LOCUS       DASYLX010000001         9191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_219784,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000001 DASYLX010000000
VERSION     DASYLX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219784"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGP27_00005"
     CDS             <1..406
                     /locus_tag="VGP27_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849733.1"
                     /translation="FLLLSSRNRFTAMLGGWLTVLAGAWFVVGRALAGPLALGDPGVP
                     VAATEAKRVALELTYFYGLGALIIFLGAIALGRLSIRSARDIRYAERSAVTTTEPVAS
                     EDVTEIQPAEVPERRRRSLGGMFGRGDRLVHR"
     gene            487..1794
                     /locus_tag="VGP27_00010"
     CDS             487..1794
                     /locus_tag="VGP27_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7849734.1"
                     /translation="MSLRIIVIVAALAVVALVITLGAWVWFGVTNDQYSQLDRRLDSV
                     SSLGDISGLLRNADIPGLGQPTPNSELVRTARVDGITISVPKDIVLPKFDNGYVNTTI
                     DGVEYRVRTFTAGKASIAIGAPVAETQRRIDELHRRVLLICAGVIVGTVIVASVLWLI
                     MINPFRVLAQQARVINAQSNPDEVKVRGVQEAVEIAEAVEGMLARIGDEQQRTRAALE
                     SARDFAAVASHELRTPLTAMRTNIEVLSTLDLPDEQRQEVIADVMRTQNRIEATLSAL
                     ERLAQGELTTVADFVPVDITELLDRAAHDAMRNYPDLAVSLASSTTVLMVGMPAGLRL
                     VIDNAITNAVKHGGATEIRLAAISSVDGVEITVDDNGLGVPEEERAAVFDRFSRGSTA
                     ARSGSGLGLALVAQQAEIHGGTAALETSPMGGARLLLRLPPPS"
     gene            complement(1870..3210)
                     /gene="gabT"
                     /locus_tag="VGP27_00015"
     CDS             complement(1870..3210)
                     /gene="gabT"
                     /locus_tag="VGP27_00015"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEV7849735.1"
                     /translation="MSILEQSRHLATSIPGPRSKELIDRKLAAVSRGIGNTMPVYTAR
                     AGGGIVEDVDGNRLIDLGSGIAVTTIGNASPRVVDAVREQVADFTHTCFMITPYEEYV
                     AVAEALNRLTPGSYEKRSALFNSGSEAVENAIKIARTYTRKQAVVSFDHAYHGRTNLT
                     MALTAKAMPYKHGFGPFAPEIYRAPLSYPFRDAEFGKEMATDGEVAARRAITVIEKQV
                     GADNLAAVIIEPIQGEGGFIVPADGFLPALVDWCAENDVVFIADEVQTGFARTGAMFA
                     CEHEGIEPDLIVTAKGIADGLPLSAVTGRAEIMDAPHVSGLGGTYGGNPVACAAALAT
                     IETIEADGLVARAAEIEQVMKDRLHRMQAEDDRIGDVRGRGAMVAMEIVKSGTTEPDG
                     ELTMKLAVGCHMAGVIVLTCGTYGNVLRFLPPLSISDDLLMEGLDVLALVLADL"
     gene            3446..3796
                     /gene="yajC"
                     /locus_tag="VGP27_00020"
     CDS             3446..3796
                     /gene="yajC"
                     /locus_tag="VGP27_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV7849736.1"
                     /translation="MDLVALLPLLIILGAFMFFASRRQKKAMQKTIDLHNSLKVGDRV
                     HTTSGLQAEIKAITDDNVELEIAPGVVTTWMKLAVRDRIEPDDEFDDDTDDAGEPEST
                     ATEITDSTDQNRLT"
     gene            3876..5624
                     /gene="secD"
                     /locus_tag="VGP27_00025"
     CDS             3876..5624
                     /gene="secD"
                     /locus_tag="VGP27_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEV7849737.1"
                     /translation="MASPSTPVHPARYLAIFLALLVGVYLLVFLTGDKHAAPKLGIDL
                     QGGTRVTLTARTPDGSRPTREALNQAQQIISSRVNGLGVSGSEVVIDGDNLVITVPGN
                     DSSEARNLGQTARLYIRPVIHVIPAQEAQAGPAQSGPAQGGPPAGQPGAPAGQPGAPA
                     GQPAAPVGEPGAPQAPAEQPTGEAPQAPQPRPYPDAPPPSPTPPGPAPKPAEKDLAQR
                     IADEKALRQSTEQQIQILALQFQATRCGEDDVLAGNDDPNLPLVTCSTDHSQVYLLDK
                     SIISGEQIKNASSGMDTQRGEYVVTVEFKPDAANVWADFTASHVGTQTAFTLDSQVVS
                     APQIEEAIPGGNTQITGQFNAQKAKELANVLKYGSLPLSFESSEAETVSATLGLTSLR
                     AGLIAGAIGLALVLVYSLLYYRVLGLLTALSLVASGAMVFAILVLLGRYINYTLDLAG
                     IAGLIIGIGTTADSFVVFFERIKDEIREGRSFRSAVPRGWVRARKTIISGNAVTFLAA
                     VVLYVLAIGQVKGFAFTLGLTTILDIVVVFLVTWPLVYLASKNTTLAKPAFNGLGAVQ
                     QIARERRVSAFATGRE"
     gene            5628..6914
                     /gene="secF"
                     /locus_tag="VGP27_00030"
     CDS             5628..6914
                     /gene="secF"
                     /locus_tag="VGP27_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEV7849738.1"
                     /translation="MAKRTSDVTESSAVEAPALESSAAEAPRHGFFVRLYTGTGAFEV
                     IGRRKLWYTISGLIVAVCLASIILRGFTFGIDFEGGTKVSMPAAGVSTERIDEVFRQA
                     LGKDPESVVIVGSGPSATVQIRSEALSNEETTKLRDALFEAFHPKGLDGQPSKQAISD
                     SAVSETWGGQITHKALLALVVFLVLAAIYIALRYERYMALAAMATLVFDLVVTAGVYS
                     LVGFEVTPATVIGLLTILGFSLYDTVIVFDKVEENTHGFQHTTRRTFAEQANLAINQT
                     FMRSINTSLISVLPIISLMVVAVWLLGVGTLKDLALVQLVGVVVGTYSSIFFATPLLV
                     SLRERTELVRTHTRRVLNRRKPAAGKSRAAAEPVDADAADADAEKETVAAASTATPTG
                     TGKHAVADKPAPGARPIRPTASRTGRPAGKRNTRRR"
     gene            6923..8563
                     /locus_tag="VGP27_00035"
     CDS             6923..8563
                     /locus_tag="VGP27_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7849739.1"
                     /translation="MTARLRGLAAAVTVALVSAVGLSSCSSRPASDITYAVDGTLTTY
                     NTNTVAGAASGGPQAFARVLTGFNYHGPDGQIVGDHDFGTIAVVGRAPLVLDYVISDK
                     AVYSDGKPVTCDDLVLAWASQSGRFPGFEAANQAGYRDIASVDCAPGQKRARVSFAAD
                     RALVDFGQLFAATSLMPAHVISDELGVDVTSAIINNDGPSIDRIAQAWNSTWNLTPKL
                     DVKRFPSSGPYKLDSVSDDGAVVLVANDKWWGAKPVTNKITVRPRGADVQDRLNNGTV
                     DVVDVAAGSSGVLNMPEDYAQTDSPSAGIEQLIFAPRGPLAAPPARRAVALCTPRDVI
                     ARNAEVPIANTRLNPVTEEAIGSGEAVGAEQFIAANPGAARDALGNQPLAVRIGYQTP
                     NARLAATVGAIAKSCAAAGIVVKDVASETTGPLTLRNNEIDVLLSSTGGATGSGSTGS
                     SAMDAYDLHTGNGNNLAGYSNPQIDGIIDSLAVTADPKEVARLLGNAGPVLWADLPTL
                     PLYRQQRSLITSKKMYAVSSNPTRWGAGWNMDRWVLEK"
     gene            8518..9123
                     /locus_tag="VGP27_00040"
     CDS             8518..9123
                     /locus_tag="VGP27_00040"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV7849740.1"
                     /translation="MGSGMEHGPLGAGEVSAGGQGRSAPVDNSEVKAVIESLTREVAD
                     FPEPGVQFKDLTPVLADPHGLAVVTAALAQFAAGAELVAGIDARGFLVGGAVANKLGV
                     GVLAVRKAGKLPPPVYSETYDLEYGSATLEVPADGIDLTGRNIVIIDDVLATGGTLAA
                     TTRLLTAAGAKVTGAVVVLELAALKGRDLLEPLPVNSLHTV"
BASE COUNT         1555 a         3124 c         3033 g         1479 t
ORIGIN      
        1 tttcttgctg ctgagttcgc gcaaccggtt cactgcaatg ctcgggggct ggttgaccgt
       61 gctcgccggg gcctggttcg tggtggggcg agcgctggcc gggccgctgg ctctcgggga
      121 ccctggggta ccggtggccg caaccgaggc caagcgcgtg gccctggagc tcacctactt
      181 ctacgggctg ggcgcgctga tcatcttcct cggtgcgatt gcgctcggcc ggttgtcgat
      241 tcgcagtgcg cgcgacatcc gctatgcgga gcggtcggcc gtcaccacca ccgagccggt
      301 ggcatcggag gacgtgacgg agatccagcc ggccgaggtg ccggagcgca ggcgccgctc
      361 gttgggcggc atgttcggcc gcggtgaccg tctcgtccac cgataggttt tgcgtccgct
      421 cacccggggt ggcactgtgc cctagtgcgt ctagtcaagg gcctttatcg gctgctggcg
      481 cgcgtcatgt cgctgcgcat catcgtcatc gtcgcggcgc tcgcggtggt cgctctggtc
      541 atcacgcttg gcgcgtgggt ctggttcggc gtgaccaacg accagtacag ccagctcgac
      601 cggcgcctgg actcggtcag cagcctcggc gacatcagtg ggttgttgcg caacgccgac
      661 atcccggggc tcggccaacc gactccgaac agcgaactcg tgcgcaccgc acgcgtcgac
      721 ggcatcacca tctcggtgcc gaaggacatc gtcctgccga agttcgacaa cggctatgtc
      781 aacacgacga tcgacggcgt ggagtatcgc gtccgcacct tcaccgccgg aaaggcgtcg
      841 atcgcgatcg gcgctccggt ggcggagacg caacggcgca tcgacgagct gcaccggcgg
      901 gtgctgctga tatgcgccgg ggtgatcgtc ggcaccgtca tcgtcgcctc ggtgctgtgg
      961 ttgatcatga tcaacccgtt ccgcgtgctt gcgcagcagg cccgggtcat caacgcgcaa
     1021 tccaaccccg acgaggtcaa ggtgcgcggg gtgcaggaag ccgtcgagat cgccgaggcc
     1081 gtcgagggca tgctcgcccg catcggcgac gaacaacagc gcaccagggc cgcgctggag
     1141 tcggcgagag acttcgcggc ggtggcctcg cacgaactgc gtacgccgct gaccgcgatg
     1201 cgtaccaaca tcgaggtgct gtccaccctg gaccttcccg acgagcagcg ccaggaagtc
     1261 atcgccgacg tcatgcgcac gcagaaccgc atcgaggcca cgctctcggc gttggagcgg
     1321 ctggcccagg gtgagttgac gacggtcgcc gacttcgtac cggtcgacat caccgagctg
     1381 ctcgaccgcg ccgcccacga cgcgatgcgc aactatcccg atctggcggt atcgctggcg
     1441 tcgtccacca cggtcctcat ggtcggcatg cctgcaggtc ttcgactggt catcgacaat
     1501 gcgatcacca atgccgtcaa gcacggcgga gccaccgaaa tccgcttggc cgcaatcagt
     1561 tcggtcgacg gcgtcgagat cacggtcgac gacaacggcc tcggcgtgcc ggaggaagag
     1621 cgggcggcgg tcttcgaccg gttctcccgc ggttcgaccg cggcgcgatc cggctccgga
     1681 ctcggcctcg cgttggtggc tcagcaggcc gagatccacg gcgggacggc cgctctcgag
     1741 accagtccca tgggtggcgc ccggcttctt ctgagactgc ctccgccgag ttagccgcgg
     1801 gagttggcca ccatcggcac gcccgttcgc tcgccgcaca actgatcagg cggcggcggt
     1861 gtcgaagacc tagagatcgg cgaggacgag cgcgaggacg tcgagcccct ccatcagaag
     1921 atcgtcgctg atgctcaggg gcggcaggaa gcgcagcacg ttgccatagg tgccgcaggt
     1981 cagcacgatc acgccggcca tgtggcagcc gacggccagc ttcatggtca actcgccgtc
     2041 gggttcggtc gtgccggact tcacgatctc catcgcgacc atcgcgccgc gaccgcgcac
     2101 atcgccgatg cgatcgtctt cggcctgcat ccggtgcaac cggtccttca tcacctgctc
     2161 gatctcagcg gcccgcgcca ccaacccgtc ggcctcgatc gtctcgatgg tggccagcgc
     2221 tgccgcgcac gcgaccgggt tgccgccgta ggtgccgccg agcccgctga cgtgcggtgc
     2281 gtccatgatc tcggcgcgac cggtcaccgc cgacagcgga aggccgtcgg cgatgccctt
     2341 tgcggtgacg atcaggtcgg gttcgatgcc ctcgtgctcg caggcgaaca tcgccccggt
     2401 gcgcgcgaac cccgtctgca cctcgtcggc gatgaacacc acgtcgttct ccgcacacca
     2461 gtccaccagt gcgggcagga agccgtcggc ggggacgatg aacccgccct cgccctgaat
     2521 cggctcgatg atcaccgcgg caaggttgtc ggcgccgacc tgtttttcga tgacggtgat
     2581 agcgcggcgg gcggccactt cgccgtcggt cgccatctcc ttgccgaact ctgcgtcgcg
     2641 gaacgggtag gagagcgggg cgcgatagat ctccggcgcg aacggaccga agccgtgctt
     2701 gtacggcatc gccttcgcgg tcagcgccat ggtcaggttg gtgcggccgt gataggcgtg
     2761 gtcgaacgac accaccgcct gtttacgggt ataggtcctt gcgatcttga tggcgttctc
     2821 caccgcctcg gaacccgagt tgaacagcgc ggagcgcttc tcgtaactgc caggggtgag
     2881 acggttgagc gcttcggcga cggcgacata ctcctcgtac ggcgtgatca tgaagcaggt
     2941 gtgggtgaag tcggcgacct gttcgcgcac cgcgtcgacg acgcgcgggg acgcattgcc
     3001 gatcgtcgtg acggcgatgc ccgacccgag gtcgatcagg cggttgccgt cgacgtcctc
     3061 gacgatgccg ccgcccgccc gcgccgtgta taccggcatg gtgttgccaa tgccgcggga
     3121 caccgcggcg agcttgcggt cgatcagttc cttcgaacgc ggaccgggga tgctggtggc
     3181 gagatggcgg ctctgctcga ggatgctcac gctgttcgag cctagccggg cgcgcaccga
     3241 tccgattgga aacgccgctg cctgtcgacg ggatcacccg tcgtggcgga tcggcgcaac
     3301 cgaatccgta gctgggcggc ccagaatgcg gggccggtcg gccatcagct gctgcaatgg
     3361 cacactggtc acctagaagg ccccgatgat gcacccacgg tcacgggccg ttatcggaca
     3421 aacgacacga aagacagttg ctgccatgga tctggtcgcc ttactaccac tgctcatcat
     3481 cctgggcgcg ttcatgttct tcgcttcgcg acggcagaag aaagccatgc agaagaccat
     3541 cgaccttcat aactcgctga aagtcggcga ccgcgtccac accacgtcgg gcctgcaggc
     3601 cgagatcaag gccatcaccg acgacaacgt cgagctcgag atcgcgccag gcgtcgtgac
     3661 gacctggatg aagctcgccg tgagggaccg catcgagccc gacgacgagt tcgacgacga
     3721 caccgacgac gccggcgagc cagagtcgac ggccaccgag atcaccgaca gcacggacca
     3781 gaaccgactg acctgacgac tgacagtcag agactcagcg ccggcacgta ccctttgcgg
     3841 tgctgacgac ctgacttcga ggagaacaaa gaaacgtggc atcgccttct acgccggtgc
     3901 accctgcccg ctacctggca atcttcctcg cgctgctggt cggcgtgtac ctcctggtct
     3961 ttctgaccgg cgacaagcac gccgcgccca aactcggcat cgacctgcag ggcggcacaa
     4021 gggtgacact gaccgcccgt acgcccgacg ggtcgcgacc cacccgggag gcgctgaacc
     4081 aggcgcagca gatcatctcc tcgcgcgtga acggtctcgg tgtttcggga tccgaggtgg
     4141 tgatcgacgg ggacaacctg gtgatcaccg tgcccggcaa cgacagcagc gaagctcgca
     4201 atctgggcca gacggcgcgg ctgtacatcc gccccgtgat ccatgtcatc cccgcgcagg
     4261 aggcgcaggc cggcccggcg caaagcggcc ccgcgcaggg cggaccgccg gcaggccagc
     4321 ccggtgcgcc ggcaggccag cccggtgcgc ccgcaggcca gcccgccgcg cccgtcggcg
     4381 agcccggagc accccaggcg ccggcagagc agcccacagg ggaggcgccg caggctcccc
     4441 agccgcgccc gtaccccgac gccccgccgc cgagcccgac gccgcccggg ccggctccaa
     4501 agcctgcgga gaaagacctc gcgcagcgca tcgccgacga gaaggcgttg cgccagagca
     4561 ccgagcagca gatccagatt ttggcgctgc agtttcaggc cactcgctgc ggcgaggacg
     4621 acgtcctggc aggcaatgac gatccgaatc tgccgctggt gacctgctcg accgaccact
     4681 cgcaggtgta cctgctggac aagtcgatca tcagcggcga gcagatcaaa aacgccagtt
     4741 ccggcatgga cacccagcgc ggcgagtacg tcgtgacggt ggagttcaag ccggacgccg
     4801 ccaacgtctg ggccgacttc accgcttcgc atgtcggcac gcagaccgcg ttcacccttg
     4861 actcgcaggt cgtcagcgcc cctcagatcg aggaggcgat cccgggtggc aacacccaga
     4921 tcaccggcca attcaatgcc cagaaggcca aagagctggc caacgtgctc aagtacggct
     4981 cgcttccgtt gtcgttcgag tcgtctgaag ccgaaacagt ctcggcaaca ttgggtttga
     5041 cgtcgctgcg ggccggactc attgcgggtg ccatcggatt ggcgctggtg ctcgtctact
     5101 cgctgctgta ctaccgggtc ctcggattgc tgacggcgct gtcgttggta gcttccggcg
     5161 caatggtttt cgccatcctg gtgttgctcg gcagatacat caactacacc ctcgacctgg
     5221 cgggcatcgc cggtctgatc atcggtatcg gtaccaccgc cgactcgttc gtggtgttct
     5281 tcgaacgcat caaagacgag atacgcgagg gtcgttcctt ccgctccgcg gttccccgtg
     5341 gttgggtgcg tgcgcgcaag acgatcatct ccggcaacgc cgtgacgttc ctggccgcgg
     5401 tggtgctcta cgtcctggcc atcggtcagg tgaagggctt cgcgttcacc ctcggcctga
     5461 cgaccatcct cgacatcgtg gtggtcttcc tggtgacgtg gccactggtg tacctggcgt
     5521 cgaagaacac gacgctggcc aagcctgcct tcaacggcct cggcgccgtg cagcagatcg
     5581 cacgcgagcg acgagtttcc gcattcgcga cgggacggga atagccgatg gctaagcgca
     5641 catccgacgt gaccgaatcg agcgccgtgg aggcgcctgc cctcgagagc tcagcggcag
     5701 aggcgccgcg gcacggcttc ttcgtcaggc tctacaccgg caccggggcc ttcgaggtga
     5761 tcgggcgccg caagctctgg tacacgatca gcggtctgat cgttgcggtc tgcctggcca
     5821 gcatcatcct gcgcgggttc accttcggta tcgacttcga gggcggcacc aaggtgtcca
     5881 tgcccgccgc gggcgtcagc accgaacgca tcgacgaggt gttccgccag gcgctgggca
     5941 aggaccccga atcagtcgtg atcgtcggca gcgggccatc ggcgacggtg cagattcgat
     6001 ccgaagcgct gagcaacgag gagacgacga agctgcgcga cgcgctgttc gaggccttcc
     6061 atcccaaggg tctcgacggc cagccgagca agcaggcgat cagcgactcc gctgtctccg
     6121 agacctgggg cggccagatc acccataagg cgctgttggc cctcgtcgtc ttcctggtgc
     6181 tcgcggcgat ctacatcgcc ttgcgctacg agcgctacat ggcgctggcc gcaatggcga
     6241 cgctggtctt cgacctggtc gtcaccgcgg gggtgtactc cttggtggga ttcgaagtca
     6301 ctcctgccac cgtgatcggc ctgttgacca tcctcggctt ctcgctgtac gacacggtca
     6361 tcgtgttcga caaggtcgag gagaacacgc acggcttcca gcacaccacc agaaggacct
     6421 tcgccgaaca ggcgaacctg gcgatcaacc agacctttat gcggtcgatc aacaccagcc
     6481 tcatctcggt gctgccgatc atctcgctga tggtcgtcgc cgtgtggctg ctgggtgtgg
     6541 gcaccctcaa ggaccttgcg ctggttcagc tggtcggcgt cgtcgtcggc acctattcgt
     6601 cgatcttctt cgcgacgccg ctgctggtct cgctccgcga gcgcaccgag ctggtccgca
     6661 cccacacgcg ccgggtgctc aaccgccgca agccggcggc aggcaagtcg cgcgcggccg
     6721 ccgagccggt cgacgccgat gccgccgacg cggacgccga gaaggagacc gtcgcggccg
     6781 cctccacggc gacgccgacg ggcacgggaa agcacgcggt cgcggacaag cctgcgccgg
     6841 gtgcgcggcc catccggccc accgccagcc gaaccggacg gcccgcgggt aagcggaaca
     6901 cccggcggcg gtagcggaac acatgacggc ccgcttgcgg gggttagccg ccgcggtgac
     6961 agtcgcgctg gtgtccgccg tgggcctgtc gtcctgttcg tcgcgcccgg caagcgatat
     7021 cacctacgcc gtcgacggca cgttgacgac ctacaacacc aacaccgtcg ccggtgcggc
     7081 gtcgggcggc ccgcaggcct tcgccagggt gttgaccggc ttcaactatc acggtcccga
     7141 cggccagatc gtcggcgatc acgacttcgg caccatcgcg gtcgtcggcc gcgcgccgct
     7201 agtgctcgac tatgtgatca gcgacaaggc ggtgtactcc gacggcaagc ccgtcacctg
     7261 cgacgacctc gtgcttgcct gggcgtcgca gtccggccgg ttccccggct tcgaagcggc
     7321 gaatcaggcg ggctaccgcg atatcgccag cgtcgactgc gcgccggggc aaaagcgggc
     7381 ccgcgtgtcg ttcgcggccg accgggcact ggtcgacttc gggcagttgt tcgccgccac
     7441 gtcgctgatg cccgcacacg tcatctccga cgaactcggt gtcgatgtca ccagcgcgat
     7501 catcaacaat gacggcccga gcatcgaccg cattgcgcag gcgtggaaca gcacctggaa
     7561 cctcacgccc aagctcgacg tgaagcggtt tccgtcatcg gggccctaca agctggattc
     7621 ggtcagcgac gacggcgccg tggtgcttgt cgccaacgac aagtggtggg gcgcaaagcc
     7681 cgtcaccaac aagatcacgg tcagaccgcg aggcgccgac gtccaggatc ggctgaacaa
     7741 cggcaccgtg gatgtcgtcg acgtcgcggc cgggtcgtcg ggtgtgctca acatgcctga
     7801 ggactacgcg cagaccgaca gcccgtccgc gggcatcgag cagctgatct tcgccccccg
     7861 gggaccgctg gccgcaccgc ctgcccggcg ggcggtcgcg ttgtgcacgc cccgcgatgt
     7921 gatcgcccgc aacgcggaag tgccgatcgc caacacccgc ctcaacccgg tgaccgagga
     7981 agcaatcgga agcggcgaag cggtcggggc cgagcagttc attgcggcca accccggcgc
     8041 cgcacgggac gcactgggca atcagccgct cgccgtacgg atcggctacc agactcccaa
     8101 tgcacggctc gccgcgacgg tcggtgcgat cgccaaatcc tgcgcagccg cgggcatcgt
     8161 ggtgaaggac gtcgcctccg agaccaccgg cccgctgacg ttgcgcaaca acgaaatcga
     8221 cgttctactg tcgagcaccg gaggggccac cggcagcgga tcgaccggct cgtcggcgat
     8281 ggacgcctac gacctgcaca ccggcaacgg aaacaacctg gccggctact cgaatccgca
     8341 gatcgacggc atcatcgact cgcttgcggt gacggccgac cccaaggagg tcgcccggct
     8401 gctcggcaat gcagggccgg tgctgtgggc cgacctaccg acgctgccgc tgtatcgaca
     8461 gcagcgaagc ctgatcacat cgaagaagat gtatgccgtg agcagcaatc cgacgcgatg
     8521 gggagcggga tggaacatgg accgctgggt gctggagaag tgagcgccgg ggggcagggt
     8581 cgaagcgccc cggtagacaa ttccgaggtc aaggccgtca tcgagtcgct gacccgcgag
     8641 gtcgccgact tccccgagcc gggcgtccag ttcaaggatc tgacgccggt actcgccgat
     8701 ccgcacgggt tggccgtggt gacagccgcg ctggcccagt tcgccgcggg cgccgaactg
     8761 gtcgcgggta tcgacgcaag gggcttcctg gtcggtggcg cggtcgccaa caaactcggc
     8821 gtcggtgtgc tcgccgtgcg caaagcgggc aagctgccgc ccccggtgta cagcgagacc
     8881 tatgacctgg aatacggatc ggcgacgctc gaggtgcccg ccgacggcat cgacctgacc
     8941 gggcgcaaca tcgtgatcat cgacgatgtg ctggccaccg gcgggaccct ggcggccacc
     9001 actcggctgc tcaccgcggc gggcgcgaag gtcaccggcg ccgtcgtcgt actcgaactg
     9061 gccgccctca aaggtcgcga cctgctcgaa ccgctacccg tcaacagctt gcacaccgtc
     9121 tgaagcgata tcctcgaagt ctggctgcgc cgatgagcgc ttgcgcgacg agcagcggat
     9181 caggaggtga c
//
LOCUS       DASYLX010000002         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2194916,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000002 DASYLX010000000
VERSION     DASYLX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194916"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGP27_00045"
     CDS             <1..328
                     /locus_tag="VGP27_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7849741.1"
                     /translation="EELRRAALPRLEAMLDRCVQQGLTGLAADIVSYTLVDWGFEPST
                     VTAKTLLLYGGADATVGAAHARWYKDRVRNSRIEVVPDFGHLLIIPMWDRVLSHLAPG
                     SRSKQP"
     gene            350..439
                     /locus_tag="VGP27_00050"
     tRNA            350..439
                     /locus_tag="VGP27_00050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Ser,seq:cga)
     gene            879..1931
                     /locus_tag="VGP27_00055"
     CDS             879..1931
                     /locus_tag="VGP27_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7849742.1"
                     /translation="MAHAAEKTSDATPQISLRGLRKTFGAVTAVDGVDLDVYDGEFFA
                     MLGPSGSGKTTVLRMIAGFETPTSGSVFLDGADATGLPSYRRDVNTVFQDYALFPHMT
                     VAKNIEYGLVVDKVPKSERVRRLGEALEMVRLGGFGDRRPTQLSGGQRQRVALARALV
                     KRPKVLLLDEPLGALDLKLREQMQLELKAIQRDVGITFVFVTHDQDEALTLCDRLAVF
                     NDGRIEQLGPAQEVYEFPATAFVAGFVGSSNFIGPHVAREVLGIAGSFAVRPEKVAIV
                     PRGKTVVIGAPRVDGVVRDVVYAGAVTRIIVDGPAGLTLSSTVLNTAGELDVSIRRGT
                     QVTLSWPAAALRRISD"
     gene            2007..3197
                     /locus_tag="VGP27_00060"
     CDS             2007..3197
                     /locus_tag="VGP27_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7849743.1"
                     /translation="MFRTAGAGAAVVLAAALALSGCSSNSTPGATQAEPPKRTMASSV
                     GSGEGQLNLIAWAGYTEDGSNDKTVDWVHPFEAQTGCTVHTTYGATSDDMVQLMRTGQ
                     YDGVSASGDATLRLIYAGDVAPVNTKLVPNYKTISSFLKNGNWNSVNGQMYGIPHGWG
                     ANLLMYTPSVVTPAPTSWSAVFDDASKYKGKVTAYDSPIYIADAALYLKSSDPSLGIT
                     DPYSLTTKQLDAAVTLLKKQNASVGEYWSDYTKAVQAFESGTSVVGTTWQVIANLINS
                     DATVKVETVVPKEGVTGWSDTWMISSKSKHPNCMYKWMNYITSPSVNAQVTEWYGEAP
                     AQTLACAEMSDPNFCADYHATDEAYAAKIHYWTTPTKQCVDGKGDNCTSYTDWVNAWQ
                     QIRG"
     gene            3245..4051
                     /locus_tag="VGP27_00065"
     CDS             3245..4051
                     /locus_tag="VGP27_00065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7849744.1"
                     /translation="MALLLSVPLLWLVVMYLGSIVVMLVSAFWTVDSFTGAVVQTFTL
                     DNIVRLFTDPLYGTVALRTILVAVGVTLIDVVIAVPMALFISKVASPRARVALLVAVT
                     TPLWASYLVKAYAWRILLAPNGPLAFVTGGNTPGYGLPATVITLAYLWLPYMIIPVYA
                     GFDRVSDSLVEASFDLGAGAWRTVRSITLPLVFPAIAAGSIFTFSLTLGDYIAVGIVG
                     GKTQLLANLIYGQLVTANNQPFAAALSLIPLAAIILYLFAMRRTGALENV"
     gene            4051..4863
                     /locus_tag="VGP27_00070"
     CDS             4051..4863
                     /locus_tag="VGP27_00070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7849745.1"
                     /translation="MKLPSALRITLYIWMGIGLAILYIPLLLIVLNSFNSSRTFGFPP
                     TGFTIEWWQRAVSSSGALSALGTSVLAGVCAMLIALALGTMAAFAVQRYTFFGRTTIS
                     FLVVLPITLPGIVTGIALNATFVQVLGPFGVALGLATVIIGHATFCIVIVYNNVQARL
                     RRMGTSLEEASSDLGANGWQSFWRVTFPGVRGALVAGAVLAFALSFDEIVVTTFTAGP
                     AIQTLPLWIFSNLFRPNQAPVVNVVAAVLAVAAVVPVWLAQRFSGDSTTSRV"
BASE COUNT          926 a         1720 c         1611 g         1009 t
ORIGIN      
        1 tgaggagctg aggcgggcgg cgcttccccg gctcgaggcc atgctcgacc ggtgcgtcca
       61 gcagggcctc accgggctcg ccgccgacat cgtgagctac accctcgtgg actggggatt
      121 cgagccgtcc accgtcacgg cgaagacctt gctgttgtat gggggagcgg atgcgacggt
      181 cggtgccgcg cacgcccgct ggtacaagga ccgcgttcgc aattcccgca tcgaagtcgt
      241 gccggatttc ggccatctct tgatcattcc gatgtgggat cgcgtgctgt cccacctcgc
      301 cccgggttcg agatcaaagc agccctagct ctgtactaag atcgtctgcg gaaccgtgtc
      361 cgagcggccg aaggtgcaac tctcgaaaag ttgtgtgggt gagagcccac cgtgggttca
      421 aatcccaccg gttccgccag cggggcccct aaagtactgc tctaagtgct gggtcctgga
      481 aacgcgttcg cagatatttg cgggcgcgtt ttcgtcttcc cggcgtcgaa cgcctctggc
      541 catggatcgt agaaggccct cagtgagcgg ggggctggat gcgatgcgtg ccgcaattcc
      601 gttcgagatc acgaagccga tctcgcgtcg cccccaatac gtcgcccacg cctcgacaaa
      661 ctcgtcgtag ctgtgcgccc ccgttgtggg tcaaccagct aaaaccgctg tcggtggtga
      721 gcgagttgcc gaccttgacg acggaatgcg cgcgtgattg cattgcctca actcaaaccg
      781 tcctcagaga ggcggaaatg actgaattcc gtaccagccg cagggccaaa ccttcggaat
      841 cagcaccaga cggcgttcat cgggcctcgc gattctccat ggctcacgcc gccgagaaaa
      901 cttccgacgc caccccgcaa atctccctgc gagggttgcg caaaacgttt ggcgccgtca
      961 ccgcggtcga cggggttgac cttgacgtgt acgacggcga gttcttcgcg atgctcggcc
     1021 cgtcggggtc aggcaagacg acagtcctcc ggatgattgc cgggttcgag acgccgacgt
     1081 caggttcggt gttcctggac ggcgcagatg cgacgggcct gccgtcctat cgccgagacg
     1141 tcaacaccgt cttccaggac tatgccctct ttcctcacat gaccgtcgcc aagaacatcg
     1201 agtacggctt ggtcgtcgac aaggtgccca agtcggagcg cgtccggcgg ctcggcgagg
     1261 cgctggagat ggtgcgcctt ggcgggttcg gcgatcgccg gcccacgcag ctctccggtg
     1321 gacagcgtca gcgcgtcgcc ctggcccgcg cacttgtcaa acggccgaaa gtgcttctgc
     1381 tcgacgagcc cctcggcgca ctcgacctca agttgcgcga gcagatgcaa cttgaactca
     1441 aagccatcca gcgcgatgtc ggcatcacat ttgtgttcgt gacacacgat caggacgaag
     1501 cactcacgct ctgcgaccgg ctcgccgtct tcaacgacgg gcgcatcgaa cagctcggtc
     1561 ccgcccaaga ggtctacgag tttccggcga cagcgttcgt ggccggcttc gttggctcat
     1621 ccaacttcat cgggccacac gtcgcgcgcg aggtgctcgg gatcgcgggc tcattcgcag
     1681 tccggcccga gaaagtggcc attgtgccac gcggcaagac tgtcgtcatc ggtgcgccca
     1741 gagtggatgg ggtcgtgcgc gacgtcgtct acgccggggc cgtcacccgc attatcgtcg
     1801 atggcccagc cggactcacc ctcagctcga cggtgctcaa cacggctggc gagctggatg
     1861 tcagcatccg gcgcggaacg caggtcaccc tgtcgtggcc ggcggccgcc ttgcgcagga
     1921 tctcggactg agcggcggca gcttcaccag caccagcaca gcttcacacc atggcacatg
     1981 aaggagaggc atacatcatg agaaggatgt ttcgcacggc aggagcgggg gcggcagtcg
     2041 tgctcgcggc cgccctggct ctgtccggat gctcgtccaa cagcacgcca ggagccacgc
     2101 aagccgagcc cccaaaaagg acgatggcgt catcggtcgg ctccggcgaa ggccagctca
     2161 acctgattgc gtgggccggg tacacggaag atggcagcaa cgacaagacg gtcgactggg
     2221 tgcatccgtt cgaggcgcag acaggctgca cggtgcacac cacctacggt gcgacctcgg
     2281 atgacatggt gcagctcatg cgcaccggac aatacgacgg ggtatccgcg tcgggcgacg
     2341 ccacattgcg actcatctac gcgggcgacg tggctccggt gaacaccaag ctcgtgccga
     2401 actacaagac gatctcgtcg ttcctgaaga acggcaactg gaactccgtg aacgggcaga
     2461 tgtacggcat cccccacggc tggggtgcca acctgctgat gtacacgccg tccgtggtga
     2521 cgccggcgcc gacatcatgg tctgccgtct tcgacgatgc gagcaagtac aaaggcaagg
     2581 tcaccgcgta cgactcgcct atctatatcg ctgacgcggc gctctacctc aaatcgtccg
     2641 acccctcact cggcatcact gacccatact cgctcacgac gaagcagctc gacgcggccg
     2701 tcacgctgct caagaagcag aacgccagcg tcggcgaata ctggtctgac tacaccaagg
     2761 cggtgcaggc attcgagtcc ggcacctcgg tcgtcggcac gacatggcaa gtcatcgcca
     2821 acctgatcaa ttccgatgcc acggtcaagg tcgaaaccgt cgtccccaag gagggcgtca
     2881 ccggatggtc ggacacctgg atgatctcgt ccaagtccaa gcaccccaac tgcatgtaca
     2941 agtggatgaa ctacatcacc tcgccctcag tgaacgccca agtcaccgaa tggtacggtg
     3001 aagcgcccgc ccagaccctt gcctgtgcgg agatgagcga ccccaacttc tgcgctgact
     3061 accacgccac cgacgaggca tatgcggcga agatccacta ctggacgacg cctaccaagc
     3121 agtgcgtcga cggcaagggt gacaactgca cctcgtacac cgactgggtg aacgcgtggc
     3181 aacagatcag gggctagctc cgacgcgctc gtccagctac ctttccacga gacccagggc
     3241 gcggttggcg ctgcttctca gcgtcccgct gctctggctc gtggtgatgt acctcgggtc
     3301 gatcgtggtc atgctcgttt cggcgttctg gacggtcgac agcttcaccg gcgccgtcgt
     3361 gcagaccttc accctcgata acatcgttcg cctgttcacc gacccgctat acggaacggt
     3421 cgcgctgcgc accatccttg tcgccgtcgg ggtgaccctg atcgacgtgg tgatcgccgt
     3481 tccgatggcg ctcttcatca gcaaggtagc gtcgccgcgt gcgcgggtgg cgctgctcgt
     3541 cgcggtcacc accccgctct gggcgagcta tctcgtgaag gcatatgcat ggcgcatctt
     3601 gctcgctccg aacggcccgc tcgcattcgt caccggcgga aacacgccgg gctacggact
     3661 cccagcgacc gtcatcacac tcgcctacct gtggctgccg tacatgatta ttccggtgta
     3721 tgccgggttc gatcgggtgt ccgactcact cgtggaggct agctttgacc tcggcgcggg
     3781 tgcctggcga acggtgcgct cgatcactct gccgctggtg ttcccggcca tcgcggccgg
     3841 ctccatcttc acattctcgc tcacgctcgg ggactacatc gcagtcggga tcgtcggggg
     3901 caagacccag ctgctcgcga acctcatcta cgggcagctg gtgacggcga acaaccagcc
     3961 gttcgcggcc gcgctctcgc tcatcccgct cgccgcgatc atcctgtacc tgttcgcgat
     4021 gcgacgcacc ggcgccctgg agaacgtctg atgaaactgc ccagcgccct gcgcatcacg
     4081 ctctacattt ggatgggcat cggcctggcc atcctctaca tccctctgct gcttatcgtg
     4141 ctgaattcgt tcaacagttc tcgcacgttc ggctttccgc ccaccggctt cacgatcgag
     4201 tggtggcaga gagccgtctc cagcagcggc gccctcagcg ccctcggcac ctcggtgctc
     4261 gccggggtct gtgcgatgct catcgcgctg gcgctcggca cgatggcggc gtttgccgtg
     4321 cagcgataca cgttcttcgg ccgaaccacc atcagcttcc ttgtggtact gccgatcacc
     4381 ctgcctggaa tcgtcaccgg catcgcgctc aacgcgacct tcgtgcaggt gctcgggccg
     4441 ttcggggtcg ctctcgggct cgccaccgtc atcatcggcc acgcgacttt ctgcatcgtc
     4501 atcgtctaca acaacgtgca ggcgcgcttg cggcggatgg gcacctcgct cgaggaggcc
     4561 tcgtctgacc tcggtgccaa cggctggcag tccttctggc gggtgacctt tcctggcgtg
     4621 cggggcgcat tagtggcggg ggcggtgctg gcgttcgcgc tcagtttcga cgagatcgtg
     4681 gtgacgacat tcacggctgg cccggcgatc cagacgctgc cgctctggat attcagcaac
     4741 ctgttccggc ccaaccaagc gccggtcgtc aacgtcgtcg ccgctgtgct ggccgtcgcc
     4801 gctgtcgttc cggtctggtt ggcgcagcgc ttctctggcg actcgaccac aagtcgggtg
     4861 tgaacggcaa cggtgcttac ctgccgacgc cgtccggagg aggcctcagc gatcgggttc
     4921 cggaggattg ggtggcttca accaccgcgc tggcgggaga gcctgaacga agacgtccat
     4981 tccgcggatt tgctcgcggt ggtcgttgcg caggacacca agacgtccac tcacctaaca
     5041 agggttcggc gatgaagctc ttacgtcctt gtcgctgtag ggattttcca caacagccac
     5101 ggaccgattt tccgcgggct ctagtgccca agtaggttga gagcaacaag gtccgtgatg
     5161 agcggaaatc gggatgctgg tccggcttgg gattctggat ttttgccgat tgaatttgct
     5221 gcccgaggtt gatcagggga ttttttcttc ttcgtcagag gactat
//
LOCUS       DASYLX010000003         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2633701,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000003 DASYLX010000000
VERSION     DASYLX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633701"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGP27_00075"
     CDS             complement(<1..343)
                     /locus_tag="VGP27_00075"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV7849746.1"
                     /translation="MSDEQPTIIYTLTDEAPLLATYALLPIIRTFTEPAGINVETSDI
                     SVAARILAEFSDRLTEDQRVPDNLGRLGELTQDPATNIIKLPNISASVPQLLAAIKEL
                     KAKGYDLPDYPG"
     regulatory      516..635
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            677..2023
                     /locus_tag="VGP27_00080"
     CDS             677..2023
                     /locus_tag="VGP27_00080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEV7849747.1"
                     /translation="MSTPEDPTVNWSFETKQVHAGQTPDSATNARALPIYQTTSYTFN
                     STEHAAALFGLAEPGNIYTRIMNPTQDVVEQRIAALEGGVAALFLASGQAAETFAILN
                     LANNGDHIVSSPRLYGGTYNLFHYTLPKLGIDVSFVEDPDDLDSWRAAVRPNTKAFFG
                     ETISNPQNDILDIPNVAAVAHEAGVPLIVDNTVATPYLIQPLSHGADIVVHSATKYLG
                     GHGAAIAGAIVDGGGFDWTNGKFPGFTTPDPSYHGVVFAELGPPAYALKARVQLLRDM
                     GSAAAPFNAFLVAQGLETLSLRVERHVANAQRVAEFLDAHPDVTSVNYAGLPGSPWYE
                     LGRKLAPKGTGAVLAFELAGGIDAGKAFVNALTLHSHVANIGDVRSLVIHPASTTHAQ
                     LTPEEQLATGVTPGLVRLAVGIEGIDDIIADLEQGFAAAKPFHATANADPAAVASF"
     gene            2033..3154
                     /locus_tag="VGP27_00085"
     CDS             2033..3154
                     /locus_tag="VGP27_00085"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV7849748.1"
                     /translation="MTIFDVPIDTLPAEGEVAVVDIGSLTLENGAVVDDVSIAVQRWG
                     ALSPNRDNVVVVLHALTGDSYITGPAGPGHPTAGWWDGVAGPGAPIDTDRWCAVATNV
                     LGGCRGSTGPSSLARDGKPWGSRFPLVSIRDQVEADVAALAALGITEVAAVVGGSMGG
                     ARALEWMVAYPDNVRSALVLAVGARATADQIGTQSTQVAAIKSDPNWCNGDYYGTGRS
                     PDAGMEIARRFAHLTYRGEVELDNRFRNDGQGAEDPAIGGRFAVQSYLEYQGRKLLSR
                     FDAGTYVALTESLSSHDVGRGRGGIRAALSNCHVPVVVGGITSDRLYPLRLQQELAEL
                     LPGCAELKVVDSIYGHDGFLVETEAVGVLIRETLELATR"
     gene            3160..3903
                     /locus_tag="VGP27_00090"
     CDS             3160..3903
                     /locus_tag="VGP27_00090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7849749.1"
                     /translation="MSRSGEEPRQQRSLSFGAEAAAYERGRPSYPPEAIDWLLPANAR
                     DVLDLGAGTGKLTTRLVERGLGVVAVDPIPEMLELLTNSLPETPALLGTAEDIPLPDD
                     SVDAVLVAQAWHWFDPERAIKEVARVLRPGGRLGLVWNSRDERLGWVKDLGRIIGPED
                     DPFSQTVTLSEPFANVERHHQEWTSYLTPQALIDLVASRSYCITSPADVRTQTLERVR
                     ELLTTHPALANTNGLALPYITVSIRATLS"
     gene            complement(3967..4128)
                     /locus_tag="VGP27_00095"
                     /pseudo
     CDS             complement(3967..4128)
                     /locus_tag="VGP27_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(4155..4448)
                     /locus_tag="VGP27_00100"
     CDS             complement(4155..4448)
                     /locus_tag="VGP27_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HEV7849750.1"
                     /translation="MSIRTFAGKVFRGQWPILLVGVFFLAAFSLVIAGYWRRGALVIG
                     IGVAVAAALRIALTDDRAGLLVVRSRGIDFVTTATVSAAVLYIAATIDPLGTS"
     gene            complement(4459..>4668)
                     /locus_tag="VGP27_00105"
     CDS             complement(4459..>4668)
                     /locus_tag="VGP27_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV7849751.1"
                     /translation="MVRPGAAVVDVGVSRDENGKLVGDVAPDVWEVAGHVSPNPGGVG
                     LLTRAFLLANVVELAERAMSPSAKG"
BASE COUNT          805 a         1594 c         1538 g          731 t
ORIGIN      
        1 ggcccggata gtccggcaag tcgtagccct tggccttcaa ctccttgatg gcggcgagca
       61 actggggcac cgaggcgctg atattcggca gcttgatgat gttggtggcc gggtcctgtg
      121 tgagctcgcc cagccggccc aagttgtccg ggacgcgctg gtcttcggtc aggcggtctg
      181 agaactccgc caggatgcgc gccgcgaccg agatgtcact ggtttcgacg ttgatgcctg
      241 ccggctcggt gaaggtccgg atgatcggca gcaaggcgta ggtcgccagc agcggcgcct
      301 cgtccgtcag cgtgtagatg atcgtcggct gctcgtcgct catgtgtggt gtctcccggc
      361 gtcagtgtcg gctatcagta tcgcgtcggt tacgaaccta cgcgtgccga agatggaggc
      421 atttgcccca tgccctgacg gagccgacac ccgcagaatt gcctcctgat catcccactg
      481 gcagcgatga cctgggttgc gataggcttc caagcggtca tgagcgccag cgtcaagccc
      541 cggcttgctg gccggcaacc ctccaaccgc ggtggggtgc cccgggagac gaccaggttg
      601 agtagccgaa cccggctacg cggcaagcgc gggtccgtcg gacgggcccc caaagtaggc
      661 aaatggaggt cctttcatga gcacgccaga agatccgacg gtcaattggt cgttcgagac
      721 caagcaggtc cacgccgggc agacgccaga cagcgccacc aacgcgcggg cgctgccgat
      781 ctaccagacc acctcgtaca cgttcaacag caccgaacac gccgccgccc tcttcggtct
      841 cgccgagccc ggcaacatct acacccggat catgaacccg acccaggacg tcgtcgagca
      901 gcgcatcgcc gccctcgagg gtggggttgc ggcgctgttt ctggcctcgg gccaggccgc
      961 cgagacgttc gcaatcctca acctggcgaa caacggtgac cacatcgtgt ccagcccgcg
     1021 gctgtacggc ggcacctaca acctgttcca ctacacgctg cccaagctcg gcatcgacgt
     1081 cagcttcgtc gaggatcccg acgacctgga ctcctggcgg gccgccgtgc ggcccaacac
     1141 caaggcgttc ttcggtgaga ccatctccaa cccgcagaac gacatcctcg acattcccaa
     1201 tgtcgcggcg gtcgcgcacg aggccggggt gccgctcatc gtcgacaaca ccgtcgccac
     1261 cccttacctg atccaaccgc tcagccacgg cgccgacatc gtcgtgcatt cggccaccaa
     1321 gtacctgggc ggacacggcg ccgcgatcgc gggcgccatc gtcgacggcg gcggcttcga
     1381 ctggacgaac ggcaagttcc ccggattcac cacgcccgat cccagctacc acggggtggt
     1441 gttcgcggag ctggggccgc ccgcatacgc actgaaggcc cgggtgcagc tgctgcgcga
     1501 catgggatca gcggcggcgc cgttcaacgc cttcctggtc gcccagggat tggagacgtt
     1561 gagcctgcgc gtcgagcgac acgtggccaa cgcacaacgg gtcgccgagt tcctcgacgc
     1621 ccacccggac gtgacatcgg tgaactatgc gggtctgccc ggttcaccgt ggtacgagct
     1681 gggccgcaag cttgcgccga agggcacggg tgcggtgctg gccttcgagc tcgccggggg
     1741 catcgatgca ggcaaggcgt tcgtcaacgc gttgacgctg cacagccacg tcgccaacat
     1801 cggcgatgtg cgctcgctgg tcatccaccc ggcgtcgacc acgcatgcgc agctgacacc
     1861 agaggagcag ctggcgaccg gtgtgacgcc cggcctggtg cggctggcgg tcggcatcga
     1921 ggggatcgac gacatcatcg ccgacctcga gcagggcttc gcggcggcca agccgttcca
     1981 cgccacggca aacgccgatc ccgccgcggt tgcgtcgttc tgaggactcg acgtgacaat
     2041 ctttgatgtg cccattgaca cgctgcccgc cgaaggtgag gtcgcagtcg tcgacatcgg
     2101 gtcactgacg ctggagaacg gcgccgtcgt cgacgacgtc tccatcgccg tgcagcggtg
     2161 gggtgcgctc tcgcccaacc gcgacaacgt cgtggtggtg ttgcacgcgc tgaccggtga
     2221 ctcatacatc accggtcctg cgggtcccgg ccacccgaca gcgggctggt gggacggcgt
     2281 cgcgggccct ggcgcaccga tcgacaccga ccggtggtgc gctgtggcca ccaacgtgct
     2341 gggcggctgc cgcgggtcga ccgggccgag ttcgctcgcg cgcgacggaa agccttgggg
     2401 ctcaaggttt ccgctcgtca gcattcgcga ccaggtggag gccgacgtcg ccgcgctcgc
     2461 ggcgctgggg atcaccgagg tggcggccgt ggtcggcggg tcgatgggcg gtgcccgtgc
     2521 actcgagtgg atggtcgcct atcccgacaa cgtccggtcg gcgctggtgc tcgcggtcgg
     2581 ggcccgtgcg accgccgacc agatcggcac ccagagcaca caggtcgccg cgatcaagtc
     2641 cgatccgaac tggtgcaacg gcgattacta cggcaccggc cgctcacccg acgccggcat
     2701 ggagattgcc cgccgcttcg cgcacctgac ctaccgcggc gaggtcgaac tcgacaaccg
     2761 cttccgcaac gacggtcagg gcgccgagga tccggcgatc ggcggccgct tcgccgtgca
     2821 gagctacctc gagtaccagg ggcgcaagct actgagccgg ttcgatgcgg gcacctacgt
     2881 cgcgctgacc gagtcgttgt ccagccacga cgtcggacgc ggccgcggag gcatccgcgc
     2941 ggcactgagc aactgccatg tgccggtggt cgtcggcggc atcacctccg accggcttta
     3001 tccgttgcgg ctgcagcagg aattagccga gctgcttccg ggttgtgccg agttgaaggt
     3061 tgtcgactcg atctacggcc acgacggctt cctcgtcgag acggaggcgg tcggggtgct
     3121 gatccgcgag acattggagc tggctacaag gtgacagcaa tgagccgctc gggcgaagag
     3181 cctaggcagc agcgttcctt gtcgttcggc gcggaagccg cggcatacga gcgcggacgg
     3241 ccgtcgtatc cacccgaagc gatcgactgg ctgctgccgg ccaacgcccg cgatgtgcta
     3301 gacctcggcg cgggaaccgg gaagctgacc acccggctcg tcgagcgggg gctcggtgtg
     3361 gttgcggtgg accccatccc ggaaatgctt gagctgctga ccaactcgct accggagaca
     3421 cccgcgctgc tgggcaccgc cgaggacatc ccgctgccgg acgacagcgt cgacgcggtg
     3481 ctcgtcgcac aggcgtggca ctggttcgac cctgaacgcg cgatcaagga ggtcgcgcgt
     3541 gtgctgcggc ccggcggacg gctgggcctg gtgtggaaca gccgtgacga gcggctggga
     3601 tgggtcaagg acctcggccg gatcatcgga cccgaggacg acccgttcag ccagaccgtc
     3661 acgctgtcgg agccgttcgc caacgtggaa cgccaccatc aggagtggac gagttatctg
     3721 accccgcagg cgctcatcga cctcgtcgca tcgcgaagct actgcatcac ctcaccggca
     3781 gatgtccgca cccaaacact ggaacgggtg cgcgagctgc tcacgacgca tccggcgctg
     3841 gccaacacga atgggttggc gctgccctac atcacggtaa gtatccgcgc cacgctctcc
     3901 tgacgcccaa actcactttt gggcgggaaa gtgcgagaag aactcgccaa aatgtcgatc
     3961 tcggcgctag ggattgtcga accaacgtgc cagcgcgcca cgcagtcgct cggcgtcacc
     4021 gggttcgaaa tcgtctgccg cccaagcgac gtagccgtcc ggacggatga gcatcgcggc
     4081 aggcccttcg gccatacccg cggccaccgt actgatagtt gcacggtggg caagtttgcc
     4141 tgttgggcaa ccggctagct ggtgcccaac gggtcgatcg tcgcggcgat gtagagcacg
     4201 gccgcgctga ccgtcgccgt ggtgacgaag tcaatgcccc tgctgcgcac gacaagcagg
     4261 cccgcccggt cgtcggtcag cgcgatgcgc agagccgccg cgacggcaac accgatgccg
     4321 atcaccaacg caccgcgccg ccagtacccg gcgatcacca ggctgaaggc ggccagaaag
     4381 aagacaccca ccagcagaat cggccactgg ccgcgaaaca ccttgccggc gaacgtccga
     4441 atgctcacgg tgggcctgtc agcccttcgc gctagggctc atagcgcgtt cggccaattc
     4501 gacgacgttg gccagaagga acgccctcgt caacagaccg acaccaccgg ggttcggcga
     4561 cacatggccg gccacctccc acacatcggg cgccacgtca ccgaccagct tgccgttctc
     4621 gtcgcggctg acgccgacat cgacgaccgc ggcccccggt cgcaccat
//
LOCUS       DASYLX010000004         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2414427,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000004 DASYLX010000000
VERSION     DASYLX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414427"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VGP27_00110"
     CDS             <1..1435
                     /locus_tag="VGP27_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV7849752.1"
                     /translation="GVPIAAVLTPAETLASEHFRSVGALTEATIAPGVDITVPVGAFV
                     VDANHCGYQRPAPAAGDNEATWATPRSDRPDISASSSGNRPFDGLRILDLGVIVAGGE
                     LGRLFADLGAEVIKIESATYPDGLRQTPPGQVMSRSWALTHRNEYGLGLDLRSPQGAD
                     VFSRLVSGADAVFANFKPGTLTSLGFSFEKLRELNPRIVLAESSAFGATGPWSARMGY
                     GPLVRATTGITSLWSSDDCPGFFDATTIFPDHVAARITAIAALASLIGREQTGTGARV
                     HISQAESAVSQLAASYVTDAAVVAGVPVDEGSAAHGVYGCAGDDEWCVISLRTAADRR
                     ALAAVMEVAGLPDDRSELVDTVSEWTSRMDKNAVAEILQQAGVPAAPMNRAADVLADP
                     VLLHRHSYTDMVHPLFEHPLPSETSPTRFAHIPPAELRPAPMPGEHTREVCHKLLSLD
                     TEEIDRLIAEGVLFTASDLDDASRTRS"
     gene            1432..2904
                     /locus_tag="VGP27_00115"
     CDS             1432..2904
                     /locus_tag="VGP27_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV7849753.1"
                     /translation="MTTPRTPVLIGYGQVNQHDETPAVEPVDLMAAAARAAADPRVLQ
                     AVDSVRIVNILSWRYRDPGRLLAQRINADDATTRYTGVGGNVPQTLVNQACLDIQAGR
                     ADVVLIAGAETFRTRTRLRNRGEKPNWTSEGESVPFAEGANEGQPLVGPAESRIELNR
                     APYFYAMFEQALRIDAGEQPDTHRARIGELWARFSAVAAGNPHAWSRKALPGEEIWQP
                     GTDNRMISFPYTKLMNSNNMVDQGAVIILASAEKATHLQIPSERWVFPYAGTDAHDTF
                     AIAERAELHRSPAIRIAGRRALELAGVGVDDLDLIDVYSCFPSAVQVAAAELALSLDD
                     TDRPLTVTGGLTFAGGPWNNYVTHSIATMAERLVANPGQRGLITANGGWLTKHSFGVY
                     GTKPPQHEFRWEDVQSTVDAEPTSKAEVEWSGSGTVEAWTTPYDRSGAPEKVYLAVRT
                     PNDSRTLAVIIDRGEAEATVREDIAGATVTVHPDGTANLS"
     gene            complement(2930..3505)
                     /locus_tag="VGP27_00120"
     CDS             complement(2930..3505)
                     /locus_tag="VGP27_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849754.1"
                     /translation="MRTITLISAALLTVTGCSAERVVNTGEWESVTATSTSVAPSLPP
                     TNPVHLANAFDFVQTPNGQTAYYFSTPSGRWQCAILPHDKAACQATSGAIGVEGAPDT
                     VPDAAGGQTTPNAIVIEPEGDAHFDAIAGPSPAAATVLPFNRTLVVARFRCNVQEATG
                     VSCLSEESGAGFTFSADGFKPQYSEVPLDAP"
BASE COUNT          610 a         1219 c         1197 g          584 t
ORIGIN      
        1 tggcgtgccg atcgcggcgg tcttgactcc tgcggagaca ctcgcatccg aacacttccg
       61 gtcggtcggc gcgctgaccg aggccacgat cgcacccggc gtggacatca cggtgcccgt
      121 cggcgcattc gtcgtcgacg ccaaccattg cggctaccag aggcccgcgc ctgcggcggg
      181 cgacaacgaa gctacttggg ccacaccgcg atccgaccgc ccggacatat cggcctcgtc
      241 gagcggcaat cgtccgttcg acggtctgcg gattctcgat ctcggagtga tcgtcgcggg
      301 cggtgaactc ggccgcctct tcgctgacct cggcgccgag gtcatcaaga tcgaaagcgc
      361 cacgtatccg gacggtctgc gtcagacgcc gcccgggcag gtgatgagcc ggtcgtgggc
      421 gctgacccac cgcaacgagt acggcctcgg gctggacttg cgcagcccgc agggcgccga
      481 cgtcttctcg cgcctggtct cgggtgccga tgcggtgttc gccaacttca agccgggaac
      541 gcttacctct ctgggctttt cgttcgagaa actgcgcgag ctgaatccgc ggatcgtgct
      601 ggcggagagc agcgccttcg gcgccaccgg gccgtggagc gcccgcatgg gatacggacc
      661 ccttgtacgg gccaccacag gcatcaccag tctgtggtcg tccgacgact gtcccggttt
      721 cttcgatgcc accacgatct ttcccgacca cgtggcggcg cgcatcaccg cgatcgccgc
      781 gctggcgtcg ctgatcggcc gcgagcagac cggaaccggt gcgcgggtgc acatctcaca
      841 ggcagagtcc gcggtcagcc agctcgccgc cagctatgtg accgatgccg ccgtcgtcgc
      901 cggggtgccc gtcgacgagg ggagcgcggc gcacggcgtg tacgggtgtg ccggcgacga
      961 cgaatggtgt gtgatctcgc tacgcaccgc cgccgaccgc cgagcgttgg ccgccgtgat
     1021 ggaggtggca ggactgcccg atgaccgctc cgaactcgtc gatacggtgt cggaatggac
     1081 gtcgcggatg gacaagaacg ccgtcgcaga gatccttcag caggcagggg tgcctgccgc
     1141 tccgatgaac cgcgcagccg atgtgctggc cgatcccgtt ctgctgcata gacattcgta
     1201 caccgatatg gtgcacccac tgttcgagca tcctttgccc tccgaaacga gcccgacgcg
     1261 gttcgcccac atcccgccgg ccgagttgcg ccctgccccc atgcccggcg agcacacccg
     1321 cgaggtctgc cacaaactgc tgtcgctcga caccgaggag atcgaccgcc tgatcgccga
     1381 gggcgtgttg ttcaccgctt ccgacctcga cgacgcatcg agaactcgat catgacaaca
     1441 ccgcgtacgc cggttctgat cggttatggc caggtcaacc agcacgacga gacgccggcc
     1501 gtggagcccg tcgacctgat ggccgcggcg gcgcgcgcgg ccgccgatcc gcgggtgctg
     1561 caagcggtcg actccgtccg gatcgtcaac atcctgtcct ggcgctaccg cgatcccggc
     1621 cgactgctcg cacaacgaat caacgccgac gacgcgacca ctcgctacac gggtgtgggc
     1681 ggcaacgtgc cgcagacctt ggtcaaccag gcgtgcctgg acatccaggc cgggcgggcc
     1741 gacgtcgtgt tgatcgcggg cgccgaaacg ttccgcacgc gtacgcgatt gcgcaaccgc
     1801 ggagaaaagc cgaactggac cagtgaaggc gaatcggtgc cgttcgccga gggcgccaac
     1861 gagggtcagc cgctggtcgg cccggcagag tcccgcatcg aactcaaccg ggcgccgtac
     1921 ttctacgcaa tgttcgagca ggctctgcgc atcgatgcgg gcgagcagcc cgatactcac
     1981 cgcgcccgta tcggcgaact gtgggcccga ttcagcgcgg tggccgcggg caacccgcat
     2041 gcatggagcc gtaaggcgct gcccggcgag gagatatggc aaccgggtac cgacaaccgg
     2101 atgatcagct tcccctacac aaagctgatg aactccaaca acatggtcga tcagggcgcg
     2161 gtcatcatcc tggcctcagc ggagaaggcc acgcacttgc agatcccctc ggagcgttgg
     2221 gtattcccgt atgcgggcac cgatgcgcac gacaccttcg ccatcgcgga acgtgcagag
     2281 ttgcaccgct caccggccat ccgcatcgcg ggccgccgcg cgctcgagct cgccggcgtc
     2341 ggcgtcgacg acctcgatct gatcgatgtg tattcgtgct tcccatcggc ggttcaggtc
     2401 gccgcagcgg aactggccct gtcgctggac gacacggacc gaccgctgac cgtcaccggc
     2461 ggcctgacct tcgcgggcgg tccgtggaac aactacgtca cacattcgat cgccacgatg
     2521 gccgagcggt tggtcgcgaa cccgggtcag cgcggtctga tcaccgccaa cggcggctgg
     2581 ctcaccaaac acagcttcgg tgtctacggc acgaaaccac cccaacacga attccgttgg
     2641 gaagacgtgc agtccacggt cgacgccgaa ccgacaagca aggccgaggt ggagtggtcg
     2701 ggttcgggca ccgtggaagc ctggacgaca ccgtacgacc gcagcggagc cccggaaaag
     2761 gtatatctgg cggtccgcac gcccaacgac tcgcgaaccc tcgcggtgat catcgacagg
     2821 ggcgaggcgg aggccacggt gcgcgaggac attgcgggcg cgacggtgac ggtgcatccc
     2881 gatggcaccg ccaacctcag ctgagcgaag gcttccgatt gcgacgccgc tagggcgcgt
     2941 cgagcggtac ttcgctgtac tgcggtttga atccgtcggc gctgaacgtg aagcctgcgc
     3001 cgctttcctc gctcaggcac gacacgccgg tcgcttcctg gacgttgcat cggaatcggg
     3061 caacgacaag cgtccggttg aacggcagta cggtggccgc cgccggcgag ggcccggcta
     3121 tggcgtcgaa gtgagcgtcg ccctccggct cgatgacgat cgcgttggga gtcgtctgcc
     3181 cgcccgcggc gtcgggcact gtgtcaggtg cgccctcgac gccgatcgcg ccggatgtgg
     3241 cctgacaggc cgccttgtcg tggggcagta tcgcgcactg ccagcggccg ctgggcgtgg
     3301 agaagtagta ggccgtctga ccattcgggg tctggacgaa gtcgaacgcg ttggccagat
     3361 gcacgggatt cgtgggtggc agcgacggtg cgacggacgt gctcgtcgct gtgacgctct
     3421 cccattctcc ggtattgacg acgcgctcgg ccgagcagcc cgtaacggtc aatagcgccg
     3481 ccgaaatcaa ggttatcgtc cgcatttcga acgtcagctt gccggacgcc gatcttggcc
     3541 gttcagccgg gggttagcaa tgcgcgtagc cgccgagcgt tgcgcaccgc gtgcccgccg
     3601 aggtcgttgt
//
LOCUS       DASYLX010000005         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3292019,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000005 DASYLX010000000
VERSION     DASYLX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292019"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGP27_00125"
     CDS             <1..301
                     /locus_tag="VGP27_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7849755.1"
                     /translation="ENVVTAANIFSTSPSIEQNKLVVLEDPKNVFLAANVVPLVASQK
                     MSNQLKTVLDAVSAKLTTEALIDMNTAVEGNQGIDPDEAARKWIKANGFDKPVQK"
     gene            307..1413
                     /locus_tag="VGP27_00130"
     CDS             307..1413
                     /locus_tag="VGP27_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7849756.1"
                     /translation="MINFENVTKKYPDGTVAVDDLTLEVPEGTLTVFVGPSGCGKTTS
                     MRMINRMIEPTSGTLTVNGEDVTKVDPVKLRLGMGYVIQSAGLMPHLRVVDNVATVPV
                     LRGETRRSARKAALGVMERVGLDPKLGDRYPSQLSGGQQQRVGVARALAADPPILLMD
                     EPFSAVDPVVREDLQAEIVRLQSELRKTIVFVTHDIDEAVKLGDKVAVFGRGGALLQY
                     ADPQFVLSNPANELVAGFVGADRGYRGLQFFHATGLPLHDVRSVTESDVDALALGPEE
                     WALVIKDGKPFAWINAEGVDVHRKGSSLYDSTIGGGSFFRPDGTLRLALDAALSSPTG
                     LGVAVDDDGQLIGGVRADDVIKLLDEQRRVPEVG"
     gene            1417..2106
                     /locus_tag="VGP27_00135"
     CDS             1417..2106
                     /locus_tag="VGP27_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7849757.1"
                     /translation="MRYLFTHLDDLWTLTVIHLRLSLIPIVLGLLIAVPLGALVQRTT
                     VLRRLTTVGASIIFTIPSLALFVVLPLIIPTRILDEANVIVALTLYTVALLVRSVPEA
                     LDAVSPAVLDAATAVGYKPLTRMLKVELPLAIPVLIASLRVVAVTNISMVAVGSVIGI
                     GGLGTWFTEGYQSNKSDQIIAGIIAIFVLAIVIDALIMLAGRLITPWTRAARAGGTAR
                     PKATATVGEAP"
     gene            2103..2894
                     /locus_tag="VGP27_00140"
     CDS             2103..2894
                     /locus_tag="VGP27_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7849758.1"
                     /translation="MNFLDQALSFIFTAANWAGPAGLAARIGEHLQYTVIAVFFSALI
                     AIPIGMIIGHTGRGTFLVVTGVNALRALPTLGVLLLGVLLWGLGLVPPTVALMLLGIP
                     PLLAGTYSGIANVDRAVVDAARSMGMTERRILLRVEVPNAMPLILGGLRTATLQIVAT
                     ATVAAYASLGGLGRYLIDGIKVREFYLALVGALMVTALALILDGALAFAVWASVPGSG
                     RLRKMPQPLLDDVVAFESRTPSEDRRKSTSEAGYEGGRLSPTVDG"
     gene            2891..3472
                     /locus_tag="VGP27_00145"
     CDS             2891..3472
                     /locus_tag="VGP27_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEV7849759.1"
                     /translation="MSAEPAPWPTILTWRAHDIRRMESVRVQLSNKRIKAYGRIVAAA
                     TKTHPPFSASYDLVTDESGATKRLSLTVTLAERERQLSIARDEENMWLVQDHSGQTNR
                     AAYDGALDVDVIFSPFFNTLPIRRTGLYQRSDSLTVPVVYVSLPDLAVKPVTISYNSA
                     SDGIKLHSPVADTTVTVDSEGFILDYPGLAERI"
     gene            complement(3435..>3935)
                     /locus_tag="VGP27_00150"
     CDS             complement(3435..>3935)
                     /locus_tag="VGP27_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEV7849760.1"
                     /translation="VWAMVMDLALDCEAIVVPARSDEHDAAAAAISHLPHLMAEALAA
                     TAGEVPLAFALAAGSFRDGTRVAATAPDLVRAMCEANSEQLLPALDRSIEMLAKARKS
                     LARDGSVAELVEEGHAARMRFSNFQRSQIITTVIGQNGWRDELAAAGRAGGVIKSALP
                     VRDSRG"
BASE COUNT          669 a         1285 c         1307 g          674 t
ORIGIN      
        1 cgaaaacgtc gtcaccgccg cgaacatctt cagcacgtcg ccctccatcg aacagaacaa
       61 gctcgtggtg ctggaggacc cgaagaacgt cttcctggcc gcgaacgtcg tgccgctggt
      121 ggcctcgcag aagatgtcca accaactcaa aacggtgctc gacgccgtca gtgcgaagct
      181 gaccaccgag gcgctcatcg acatgaacac cgcggtggag ggcaaccagg gcatcgatcc
      241 cgacgaggct gcgagaaagt ggatcaaggc caacggcttc gacaagcctg tccagaagtg
      301 agcgcaatga tcaattttga gaacgtcacc aagaagtatc cggacggcac ggtcgcggtc
      361 gacgacctga cgctggaggt gccggaggga acgttgaccg tgttcgtcgg tccgtccggc
      421 tgcggcaaga ccacgtcgat gcggatgatc aaccggatga tcgagccgac gtcgggcacg
      481 ctgacggtca acggcgagga cgtgaccaag gtcgacccgg tcaagctccg gctggggatg
      541 ggctacgtga tccagagcgc cgggctgatg ccgcacctgc gggtggtcga caacgtcgcg
      601 accgtgcccg tgctgcgcgg cgaaacccgg cgcagcgcac gcaaagccgc gttgggggtg
      661 atggagcgcg tcggcctcga cccgaagctg ggggaccgct acccctccca gttgtcaggt
      721 ggccagcagc agcgcgtcgg cgtggccagg gcgctggcgg cggacccccc gatcctgttg
      781 atggacgaac cgttctccgc ggtggacccc gtcgtgcgcg aggatctgca ggccgagatc
      841 gtgcggctgc aaagcgaact ccgcaagacc atcgtgttcg tcacccatga catcgacgag
      901 gcggtcaagc tcggcgacaa ggttgcggtg ttcggccggg gcggtgcgct gctgcagtac
      961 gccgacccgc agttcgtgtt gtcgaacccg gccaacgaac tcgtggcggg cttcgtcggc
     1021 gccgaccgcg gctaccgcgg cctacagttc ttccacgcga cggggttgcc gctacacgac
     1081 gtcagatcgg tgaccgaatc cgatgtcgac gcactcgccc tcgggccaga ggagtgggcg
     1141 ttggtgatca aggacggcaa gcctttcgcg tggatcaacg ccgagggtgt tgacgtgcac
     1201 cgcaagggca gctcgctgta cgacagcacc atcggcggtg gatccttctt ccggcccgac
     1261 ggcacgctga ggttggcgct cgacgcggcg ctgtcctcac cgaccggctt gggggtggcg
     1321 gtcgacgacg acggccaact gatcggcgga gtgcgagccg acgacgtgat caaactgctg
     1381 gacgagcagc ggcgtgtccc ggaggtgggg tagccggtgc gctatctctt cactcacctc
     1441 gacgacctgt ggaccctcac cgtcatccat ctgcggttgt cgctcattcc gatcgtgctc
     1501 ggtctgctga tcgccgtacc gctgggtgcg ctcgtgcaac gcacgacggt gctgcgacgg
     1561 ctcaccaccg tcggtgcgag catcatcttc acaatcccgt cgctggcgct gttcgtcgtg
     1621 ctgccactga tcatcccgac gcgaatcctc gacgaggcca acgtgatcgt ggcactgacg
     1681 ctctacaccg ttgcgctgtt ggtgcgctcg gtgcccgagg ctctcgacgc ggtgtcacca
     1741 gcggtgctcg acgcggccac cgccgtcggg tacaagcccc tgactagaat gctcaaagtc
     1801 gaactgcccc ttgcaattcc ggtgctgatc gccagcctgc gcgtcgtcgc cgtcaccaac
     1861 atctcgatgg tggcggtcgg gtcggtgatc ggtatcggcg gtctcggcac ctggttcacc
     1921 gagggttacc agtcgaacaa gagcgatcag atcatcgcag gcatcatcgc gatcttcgtg
     1981 ctggcgatcg tcatagacgc gttgatcatg ctcgccgggc ggctcatcac gccgtggaca
     2041 cgtgcggcac gggcaggcgg cacggcgcga ccgaaggcga cggcgacggt cggggaggcg
     2101 ccatgaactt cctggaccag gcgctgtcgt tcatcttcac cgcggccaac tgggcgggtc
     2161 cggccggatt ggccgcccgc atcggcgagc atctgcagta caccgtgatc gcggtgttct
     2221 tctcggcgtt gatcgccata ccgatcggca tgatcatcgg gcacaccgga cggggcacct
     2281 tcctcgtcgt caccggtgtg aacgcgctgc gggcgctgcc gacgctcggg gtgctgctcc
     2341 tcggtgtgct gctgtggggt cttggcctgg tgccgccgac ggtcgcgctg atgcttctcg
     2401 gtattccgcc gttgctggcg ggcacctact ccggtatcgc caacgtcgac cgtgcggtgg
     2461 tcgacgcggc gcgatcgatg ggcatgaccg aacgccggat ccttctgcgt gtcgaggtgc
     2521 ccaacgcgat gccgctgatt ctcggaggtc tgcgcaccgc gacgctgcaa atcgtcgcga
     2581 ccgcgaccgt cgccgcgtac gccagcctcg gtggcctcgg ccggtacctg atcgacggga
     2641 tcaaggtgcg cgagttctac ctggcgttgg tcggcgccct catggtgacc gcactcgcct
     2701 tgatcctcga cggtgcgttg gcgttcgcgg tgtgggcgtc ggtgcccggc agcgggcggc
     2761 tgcggaaaat gccgcaaccg ttgctggacg acgtagtcgc gttcgaatcg cgcacgccat
     2821 cggaggaccg gagaaaaagc acctccgaag cggggtacga aggtggccgt ctgtcgccta
     2881 cggtagacgg gtgagtgcag aacccgctcc ctggcccacc atcttgacgt ggcgcgccca
     2941 cgacattcgt cgaatggaat ccgtgcgggt gcagctgtcc aacaaacgga tcaaggcgta
     3001 cggccgcatc gtcgccgcgg caacgaaaac gcacccgcca ttctccgctt cctacgatct
     3061 ggtgaccgac gaatccggcg ccaccaagcg gctctcactg acggtgacac tcgccgagcg
     3121 cgaacggcag ctgtccatcg cacgcgacga agagaacatg tggctagttc aggaccattc
     3181 gggccaaacc aatcgggcgg cctacgacgg tgcgctggac gtcgacgtga tcttcagtcc
     3241 gttcttcaac acgctgccga tccgacgaac cggcttgtat cagcgcagcg actcgctaac
     3301 cgttccggtg gtctacgtat cgctgcccga cctggcggtg aaacccgtca cgatcagcta
     3361 caacagtgcg tccgacggca tcaagctgca ttcgccggtg gccgatacga cggtgacggt
     3421 cgactcggag ggcttcatcc tcgactatcc cggactggca gagcggattt gatcaccccg
     3481 ccggcgcggc cggcggcggc aagctcgtca cgccaaccgt tctggccgat gaccgtcgtg
     3541 atgatctgtg accgctggaa gttggagaac cgcatccggg cggcgtgacc ttcctcgacg
     3601 agttcggcga ccgagccgtc cctggccaac gatttgcgcg ccttcgccag catctcgatg
     3661 gaccggtcga gcgcaggcag cagctgttcg gagttcgctt cgcacatcgc gcgcaccaga
     3721 tccggcgcgg tggccgccac ccgcgtgccg tcccggaacg agccggcggc cagcgcgaag
     3781 gcaagcggca cctcacccgc ggtcgcggcc agagcctcgg ccatcaggtg cggcaggtgg
     3841 gagatggcgg cggccgcggc gtcgtgctcg tcggatctgg cggggacgac gatggcttcg
     3901 cagtccaacg ccaagtccat caccatcgcc cagac
//
LOCUS       DASYLX010000006         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3072601,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000006 DASYLX010000000
VERSION     DASYLX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3072601"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>2036
                     /gene="pheT"
                     /locus_tag="VGP27_00155"
     CDS             55..>2036
                     /gene="pheT"
                     /locus_tag="VGP27_00155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV7849761.1"
                     /translation="MRLPYSWLREVIQAGAPGWDVPVDELEQTLIRIGHEVEEIIPVG
                     PVSGPLTIGRVLEIEELSEFKKPIRAVKVDVGEDQPRDIVCGATNFAVGDLVVVALPG
                     TTLPGDFTITMRKTYGRTSDGMICSTAEMNLGTDHSGILVLPQGTAEPGTPAAAVLGL
                     DDTVFNVAITPDRGYCLSLRGMAREIANAYDLEYVDPAEVTPLPADGEALPVTVEPGT
                     GVLRFGLRPVTGIDPTAASPWWLQRRLLLSGIRAISPAVDVTNYVMLELGHPMHAHDR
                     SLITGGFRVRFAEPGETVVTLDGIERKLDSGDVLIVDDVATAAIGGVMGAGTTEVRDS
                     TTDVLLEAAVWDPAAVSRTQRRLHLNSEAGRRYERAVDPAISVAALDRSAALLAEIAG
                     GTAEPRLTDWRGDPPREDWSPPAVSMAVDLPDRTAGVDYPAGTTQRRLTQIGADVAVD
                     GDRVTGTPPSWRPDLRQPADLVEEVLRLEGLETIPSVLPQAPAGRGLTPAQKRRRAIG
                     KSLALNGYVEILPTPFLPAGVFDQWGLPPDDARRVTTDVLNPLEADRPQLATTLLPGL
                     LESLSRNVSRGAVDASLFAIAQVVEPSTKTHAVELIPTNRRPGDDEIAMLDASLPNQP
                     QHVGVVLTGLREPAGPWGAGRPVDASDAFEAVRIVGR"
BASE COUNT          316 a          703 c          705 g          312 t
ORIGIN      
        1 cgacatggtc gagggcgaca tccggttctc gctgccgttc ggggtaggcg cctgatgcgg
       61 cttccgtaca gctggctgcg ggaagtcata caagccggcg caccaggttg ggatgttccg
      121 gtcgacgagc tcgagcagac actgattcgc atcggccatg aggtcgagga gatcatcccg
      181 gtcgggcccg tcagcggccc gctcaccatc ggccgtgtcc tcgagatcga ggaactcagc
      241 gagttcaaga agccgatccg cgccgtgaag gtcgacgtcg gcgaggacca accccgcgat
      301 atcgtctgcg gtgcaacaaa cttcgcggtc ggggacctgg tcgtggtggc actgcccgga
      361 accacgctgc cgggcgactt caccatcacg atgcgcaaga cgtacggccg cacctccgac
      421 ggaatgatct gctcgacagc cgagatgaac ctcggcaccg accattccgg aattctcgtg
      481 ttgccgcagg gcaccgccga gccaggcacg cctgcggccg cggtgctcgg cttggacgac
      541 accgtgttca acgtcgccat caccccggac cgtggctact gcctttcgct gcggggcatg
      601 gctcgcgaga tcgcgaacgc atacgacctg gagtatgtcg atcccgccga ggtgactccg
      661 ctaccagccg acggcgaggc cctgcccgtg acggtcgagc cgggcacggg cgtgctgcgc
      721 ttcgggctgc ggccggtgac cggaatcgac ccgacggcgg cgtcgccgtg gtggctgcag
      781 cggcggctgc tgttgagcgg tatccgggcg atctcgcccg ccgtcgacgt gacgaactat
      841 gtgatgctcg agttggggca tccgatgcac gcgcacgatc gcagcctgat caccggcggt
      901 ttccgggtcc ggttcgccga gccgggggag accgtggtca ccctcgacgg catcgaacgc
      961 aagctggatt ccggcgatgt gctcatcgtc gacgacgtcg cgacggcggc gatcggcggg
     1021 gtgatgggtg cgggcaccac cgaggttcgc gacagcacca ccgatgtgct gctcgaggcg
     1081 gcggtctggg atccggccgc ggtgtcgcgc actcagcggc ggctgcacct caacagcgag
     1141 gcgggtcgcc gttacgaacg cgccgtcgat ccggccatct cggtcgccgc gttggaccgg
     1201 tccgcggcgc tgctggccga gatcgcaggc ggtacagccg aaccgaggtt gaccgactgg
     1261 cgcggtgatc caccgcgcga ggactggtcg cctcccgccg tcagcatggc cgtcgatctg
     1321 cccgaccgga ccgccggcgt ggactacccc gcgggcacca cgcagcgcag gctcacacag
     1381 atcggcgccg acgtggccgt cgacggcgac cgggtgaccg gcactccgcc gagttggcgg
     1441 cccgacctga gacagccggc agacctcgtc gaggaggtgc tgcggctgga ggggttggag
     1501 accatcccgt cggtgctgcc gcaggcgccc gccggacgcg gcctcacccc ggcgcagaag
     1561 cgccgtcgtg caatcgggaa atcgcttgcg ctcaacggtt atgtcgagat cctgccgaca
     1621 ccgtttctgc ctgcgggggt gttcgaccag tggggcctgc cgcccgacga tgcccgccgc
     1681 gtcaccaccg atgtgctcaa cccactggag gccgaccgcc cccagctggc caccacattg
     1741 ctgcccggcc tgctggaatc gctgagccgc aacgtctccc gcggagccgt cgacgcgtcc
     1801 ctgttcgcga tcgcgcaagt cgtcgagccg tcaaccaaaa cccatgccgt cgaacttatt
     1861 ccgaccaacc gcagacccgg cgatgacgag atcgcgatgc tcgacgcgtc gctgccgaac
     1921 caaccgcagc acgtcggtgt ggtgcttacc ggcctgcggg agccggcggg cccgtggggg
     1981 gccgggcgcc ctgtcgacgc cagtgacgcg ttcgaggcgg tccgcatcgt cggccg
//
LOCUS       DASYLX010000007         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2962931,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000007 DASYLX010000000
VERSION     DASYLX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962931"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="aroC"
                     /locus_tag="VGP27_00160"
     CDS             complement(<1..1053)
                     /gene="aroC"
                     /locus_tag="VGP27_00160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV7849762.1"
                     /translation="MLRWTTAGESHGRALVAVVEGMVAGVEITSAEIGRQLGRRRLGY
                     GRGARMKFEQDQITMLAGVRHGVTLGGPVAIEIGNTEWPKWETVMSADPVDPAELKDI
                     ARNEPLTRPRPGHADYAGMLKYGFDDARPVLERASARETAARVAAGTIARAFLRQALG
                     VEVLSHVIAIGASAPYDGPPPQAADLAAIDASPVRAFDKDAEARMIAEIEAAKADGDT
                     LGGVVEVVANGLPIGLGSFTSGDNRLDSQLAGAVMGIQAIKGVEIGDGFETARRRGSV
                     AHDEMYPGAAGPATSGSGPGGVMRSTNRAGGLEGGMTNGQALRVRAAMKPISTVPRAL
                     STVDMATGDEAVAIHQR"
     gene            complement(1209..1649)
                     /locus_tag="VGP27_00165"
     CDS             complement(1209..1649)
                     /locus_tag="VGP27_00165"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEV7849763.1"
                     /translation="MGARAAGACVVAWLVVLSVYDIRERRLPNWLTMPGALAILAAAT
                     SAGRGVPALIGAISLAALYLVVHVIAPAAMGAGDVKLAVGLGGLTGAFGYDAWVLAAI
                     AAPLLTAALACVSVVCRRCDDDNGVPHGPSMCVATLAATALVIA"
     gene            complement(1659..2477)
                     /locus_tag="VGP27_00170"
     CDS             complement(1659..2477)
                     /locus_tag="VGP27_00170"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV7849764.1"
                     /translation="MSSTAPDSARKAAVLGSPIAHSRSPQLHMAAYRALGLDDWTYDR
                     IECTADELPSVLRGFGPEWIGVSVTMPGKFAALRFADERTGRAELVGAANTLVRTPTG
                     WRADNTDVDGVTGALGEASGHAVVVGSGGTAPAVVVGLAELGVQRITVAARNREKAAA
                     VVDLATRLGAETGWLDIDGPELPSVVAAVSLVVSTIPADAAERYAAALSGTPVLLDTI
                     YDPWPTPLASAVEAQGGRVISGLQMLLYQAYAQVEQFTGLPAPKEAMRAAVRGD"
     gene            complement(2455..>2581)
                     /locus_tag="VGP27_00175"
     CDS             complement(2455..>2581)
                     /locus_tag="VGP27_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEV7849765.1"
                     /translation="GDWLYFVTIDLQGTTLFTRDYPQHLANIELAKRNGVLDSAR"
BASE COUNT          411 a          937 c          841 g          392 t
ORIGIN      
        1 gcgctggtga atcgcgacgg cctcgtcgcc cgtggccatg tcgacggtgg acagggcccg
       61 cggcaccgtc gagatcggct tcatcgcggc ccgcacccgc aaggcctggc cgttggtcat
      121 gccgccctcc agaccgcccg cacggttcgt cgagcgcatc acaccgcccg gcccactgcc
      181 agatgtcgcc ggcccagcgg ctccgggata catctcgtca tgagcgacgc tgccgcggcg
      241 acgcgcggtt tcgaagccgt cgccgatctc cacacccttg atcgcctgaa tacccatgac
      301 ggcgccggcc aactggctgt cgaggcggtt gtcaccgctg gtgaaagagc cgagcccgat
      361 cggcaggccg ttcgcgacga cctcgaccac gccgccgagc gtgtcgccgt ccgccttggc
      421 ggcttcgatc tcggcgatca tcctggcctc ggcgtccttg tcgaatgcgc gcaccgggct
      481 tgcgtcgatg gcggccaggt cggcggcctg cgggggcggg ccgtcatagg gggcggacgc
      541 gccgatggcg atgacgtgtg aaagcacctc gacgccgaga gcctgccgaa ggaaggcccg
      601 agcgatggtg cccgccgcga cgcgggccgc cgtttcgcgg gcactggcgc gctccagcac
      661 cgggcgggcg tcgtcgaagc cgtacttgag catgcctgcg tagtcggcgt ggccggggcg
      721 cgggcgggtg agcggctcgt tgcgtgcgat gtccttcagc tccgccgggt ccacgggatc
      781 cgcggacatc acggtttccc acttcggcca ttcggtgttg ccgatttcga tggcgacagg
      841 gccgccgagc gtcacgccgt gccgcacacc ggcaagcatc gtgatctggt cctgttcgaa
      901 cttcatccgg gcgccgcggc cgtagcccag cctgcgacgg ccgagctgac ggcctatctc
      961 cgcagacgtg atttcgacgc cggcaaccat accctcgacc acggccacca gcgcgcggcc
     1021 gtgggattca ccagcagtgg tccaacgcaa cacgcgtccc atcttcccac gtcgcagtct
     1081 gcctgcggaa atccgcgcat cctggtccgg ttcgcgatcg agatctgcat cctccgcgaa
     1141 acggaattcc agcaggtggc tactcgaact ttccctgcga cgtttccgtc tcgacgacag
     1201 cgaggcggct aagcgatgac gagcgccgtt gcggccagcg tcgccacaca catcgacggg
     1261 ccgtgcggca cgccgttgtc gtcgtcgcag cgccgacaga ccaccgagac gcatgccaac
     1321 gcggcggtca gcagcggtgc agcgatggcc gccaacaccc acgcgtcgta tccgaacgcg
     1381 cccgtcagtc cgccgagccc cacggcgagc ttcacgtcac cggcacccat cgccgcggga
     1441 gcgatcacgt gcacgactag atacagggcc gccagactga tcgctccgat caacgcgggg
     1501 acgccgcgtc ccgcagaggt ggccgctgcc agtatcgcca gcgcgcccgg catcgtcagc
     1561 cagttcggca ggcgacgctc gcggatgtcg tacacgctca gcaccacaag ccatgcgacc
     1621 acgcaagcgc cggccgccct cgcccccatg aaatgaggct aatcgccccg aactgccgct
     1681 ctcatcgcct ctttcggggc cggcagcccg gtgaactgct cgacctgtgc ataggcctgg
     1741 tacagcagca tctgcagtcc gctgatgact ctgccgcctt gtgcctcgac tgccgatgca
     1801 agcggcgtcg gccacgggtc gtagatcgtg tcgagcagta ccggcgtgcc cgagagcgcc
     1861 gcagcgtaac gctcggccgc atcggccgga atcgtgctca cgacgaggga caccgcagcg
     1921 acgacggaag gcagctcggg cccatcgatg tcgagccagc ccgtctcggc gcccagccgg
     1981 gttgcgagat cgacgacagc cgcggctttc tcgcggtttc gcgcggccac ggtgatccgc
     2041 tgcacgccga gctccgccaa tccaaccacc acggcaggcg ctgttccgcc ggagccgacc
     2101 acgacggcat ggcccgacgc ttcgcccagc gcgccggtca ccccgtcgac atcggtgttg
     2161 tcggcgcgcc atcccgtcgg cgtacggacg agggtgttcg ccgcgccgac cagctccgca
     2221 cgcccggtgc gctcgtcggc aaaccgcagc gccgcgaact tacccggcat cgtcaccgac
     2281 acgccgatcc attccgggcc gaaaccgcgt aggaccgaag gcaattcgtc cgccgtgcac
     2341 tcgatgcggt catacgtcca gtcgtcgaga cccaaggccc ggtaggccgc catatgcaac
     2401 tgcggcgaac gtgagtgggc gatcggcgag ccgagcacgg ccgccttgcg tgcgctatcg
     2461 ggcgctgtcg aggacaccgt tgcgcttcgc gagttcgatg ttcgccaggt gctgcggata
     2521 atcccgggtg aagagcgtcg tgccctgcag atcgatggtg acgaagtaca gccaatcccc
     2581 g
//
LOCUS       DASYLX010000008         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3182353,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000008 DASYLX010000000
VERSION     DASYLX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3182353"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGP27_00180"
     CDS             <1..259
                     /locus_tag="VGP27_00180"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEV7849766.1"
                     /translation="GVVVTAAAMVADVLAPGAREWWRAGHRSTEPAHSLALERLELEP
                     IVDLSMRLGEGTGAAVALPVLRAAIATLRSMATFDEASVSS"
     gene            256..981
                     /locus_tag="VGP27_00185"
     CDS             256..981
                     /locus_tag="VGP27_00185"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEV7849767.1"
                     /translation="MIRSVAGAFAFGTVLPLRAGCGVGRGVLTALPVVGVALGALAAA
                     VLWASSHAFGPGHPLAGVLAAAALLIATRGLHIDGLADTVDGLGCYGPPQRALEVMRD
                     GSAGPFGVAAVVIVIVVQGLAFAELSAVAVIAAVTAGRVAAVAACRRSVTAAEGSTLG
                     AMVANSQPVWVVGAWVVVVAAAAAPACPRPWQGPVAALMALVSSAVLVAHCVRRFGGI
                     TGDVLGAAIEVTTTVAAVGLAIR"
     gene            complement(997..2757)
                     /locus_tag="VGP27_00190"
     CDS             complement(997..2757)
                     /locus_tag="VGP27_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849768.1"
                     /translation="MAQVIGFDPAASGQQPEDVDGNPVPVGVWGDSDTGGGVFGSSGA
                     LPSGASIPIDPPAGVEGHGAAGPGVVGRSLTGNGVVGESQDGTGVLARSVASYGLLGV
                     TFAPDDSSHGVFGSSTTGGNGVTGFVGDADGVVGSSIRGAGVRGTTGNGQAGVVGENY
                     SDGSDGGFSPGVYGHSDHTVGVYGSSNVHYAIGGHTYGDTYAAYAYGGGGKPSGGVLG
                     FTYWGTGVHGQSQGAGAGPGVFGETLGNGAEAGVYGSSNANVGVRGVSIDNNGTAGLT
                     TGGASGVSGVQFSTTPGAGVSGISVLGNGVEGLTFANKRQNPDAAAVRGQSSNGFAGL
                     FVGPVRVTGNLSKTGGGFTIDHPNDPENQYLSHSFVESPDMLNVYSGTVTTNREGTAR
                     VRLPAYFETLNSDFRYQLTVIGDFAQAVIAEEVSDGALTIRTDRGRVKVSWQVTGVRQ
                     DAWAQANRIQAEQKKPRQERGKYLHPELFGAKAVGLHPAPPVRWAEVVPEQLREWADG
                     LADDLDRAGAGLARSLDKARRLLRDRADASRVQFEERGGRRAEAARNRLRERSSSGLA
                     GLEEQWRDVERMVEGMRPKR"
     gene            complement(2847..3950)
                     /locus_tag="VGP27_00195"
     CDS             complement(2847..3950)
                     /locus_tag="VGP27_00195"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV7849769.1"
                     /translation="MTDRLEFTVDRNANPASDEVRAEILSNPGFGRFHTDHMVSIDYV
                     EGKGWHNARVIPYGPIELDPSAIVLHYAQEVFEGLKAYRWADGSVVSFRADANAARLR
                     SSARRLAIPELPDEVFIESLRQLIAVDEQWVPPAGGEESLYLRPYIFASEPGLGVRPA
                     SEYRYMVIASPAGAYFPRGIKPVSVWLSHEYVRASPGGTGAAKFGGNYAASLLAQAQA
                     ADNGCDQVVWLDAIERRYVEEMGGMNLFFVFGSGGSARLVTPELSGSLLPGITRDSLL
                     QLATDAGFAVEERKIDVDEWQKKAAAGEITEVFACGTAAVITPVSHVKHTDGEFTIAD
                     GEPGEITMALRDTLTGIQRGTFADTHGWMARLS"
     gene            complement(3973..>4223)
                     /locus_tag="VGP27_00200"
     CDS             complement(3973..>4223)
                     /locus_tag="VGP27_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEV7849770.1"
                     /translation="LRGLRAVGRGVLRPELTVLDGTTPIGVTTSGTFSPTLKVGIALA
                     LLDTDHEVSDGQRVAVDVRGRPVECEVVKPPFVDAKTR"
BASE COUNT          618 a         1453 c         1412 g          740 t
ORIGIN      
        1 cggggtggtg gtgacggctg ccgcgatggt cgccgacgtg ctggcgccgg gcgcgcgtga
       61 gtggtggcgg gcgggccatc ggtccaccga gccggcgcac tcactggcgc tggagaggct
      121 ggaactcgaa ccgatcgtcg atctgtcgat gcgactcggc gagggcaccg gagcggcggt
      181 cgctttgccg gtgctgcgcg ccgcgatcgc caccctgagg tcgatggcta ccttcgacga
      241 ggccagcgtc tcgtcgtgat ccgttcggtc gcaggcgcgt tcgcgttcgg cacggtactg
      301 ccattgcgtg cgggttgcgg cgtcggccgc ggggtgttga cggcgctgcc ggtggtcggt
      361 gtggctctgg gggcgctggc cgccgcggtg ttgtgggcgt catcccacgc gttcgggccg
      421 ggccacccgt tggctggtgt ccttgcggct gcggcgctgc tgatcgcgac aaggggcctg
      481 cacatcgacg gactggccga caccgtcgac ggcctcggct gttacggccc cccgcagcgg
      541 gcgctggagg tgatgcggga cggctctgcg ggcccgttcg gggtggccgc tgtcgtgatc
      601 gtcatcgtgg tgcaggggct ggccttcgcc gaactgagcg cggtcgcggt gatcgccgcg
      661 gtgacggccg gccgggtcgc cgccgtcgcg gcatgccgcc gctcggtgac cgcggccgag
      721 ggcagcaccc tgggcgcgat ggtggcgaac agccagccgg tctgggtggt cggcgcctgg
      781 gtggtcgtcg tcgcggcggc agcagcgcct gcctgcccgc ggccatggca gggaccggtg
      841 gccgcgctta tggcgctggt atccagcgcc gtgctggtgg cgcactgtgt ccgccggttc
      901 ggcgggatca cgggtgatgt gctcggggct gcgatcgagg tgaccacgac ggtcgccgcg
      961 gtcggcctgg ccatccgcta accgaccgag caagcgctat cgcttggggc gcatgccctc
     1021 gaccatccgc tcgacgtcgc gccactgctc ctcgagtccc gcgagtccgg acgacgaacg
     1081 ttcccgcagc ctatttctgg cagcctccgc ccgccggccc ccgcgttcct cgaactgcac
     1141 gcgactggcg tcggcccggt cacgcagcag tcgccgggcc ttgtccaggc ttctcgccaa
     1201 tcccgcgccc gctcggtcca agtcgtcagc caatccgtcc gcccactcac gcagctgctc
     1261 gggaacgacc tccgcccacc ggaccggcgg agcggggtgc aggccaactg ctttcgcgcc
     1321 gaacagctcg ggatgcagat acttgccgcg ctcttgcctt ggcttcttct gttcggcttg
     1381 tatgcggttg gcctgggccc acgcatcctg ccggaccccc gtcacctgcc agctgacctt
     1441 cactcggcct ctgtcggtgc ggatggtgag agcgccgtcg ctgacctcct cggcgataac
     1501 agcctgggcg aagtcgccga tcacggtcaa ctggtagcga aagtcagagt taagtgtctc
     1561 gaagtacgct ggaagccgga ctcgcgcggt gccttccctg ttggtggtga cggtgccgct
     1621 gtagacattg agcatgtccg gcgattcgac gaacgagtgc gaaagatatt ggttctccgg
     1681 gtcattgggg tggtcgatgg tgaatccacc accggtcttg ctcaagttcc cggtaactct
     1741 caccgggccg acgaacaatc ccgcgaaacc gttggaactc tgcccgcgaa ctgctgccgc
     1801 atcgggattc tggcgcttgt tggcgaacgt cagaccttcg acgccattgc cgagcacgct
     1861 gatcccggat accccggcgc cgggtgtcgt cgaaaactgc actcctgata caccacttgc
     1921 gccaccggtg gtcaggccgg cggtgccgtt gttatcaatg ctcaccccac gcactccgac
     1981 gttggcattg ctgctgccgt agaccccggc ctcggcgcca ttgccaagtg tttcgccgaa
     2041 cacgccgggc cccgctccgg ctccttggct ctggccatga accccggtgc cccagtaggt
     2101 gaaccccaac actccgccgc tgggcttacc gccgccgccg tacgcgtagg ccgcgtaggt
     2161 gtcaccgtag gtgtggccgc cgatggcgta atgcacgttg ctgctgccgt atacgccgac
     2221 ggtgtggtcg ctgtgcccgt agacgccggg actgaagccg ccgtccgaac cgtcgctgta
     2281 gttttcgcct acgactccgg cctgaccgtt gcccgtcgtg ccgcgcacgc cggcaccgcg
     2341 gatgctgctg ccgaccacgc cgtctgcatc acccacgaaa ccggtgaccc cgttgccacc
     2401 ggtggtgctc gagccgaaca cgccgtgtga actgtcgtcc ggggcgaaag tcaccccgag
     2461 caacccgtat gacgccacac tgcgcgccag aacccctgtc ccatcctggc tttcaccgac
     2521 gacaccattg ccggtgagac tgcggccgac aacgcccggg cccgcggcgc cgtgtccctc
     2581 caccccggcg ggcggatcga tgggaatgga ggcgcccgat ggcaacgccc cgctgctgcc
     2641 gaaaaccccg ccaccggtgt cggaatcacc ccacactccg acgggaaccg gatttccgtc
     2701 gacatcctcg ggttgttgtc cgctggccgc cgggtcgaat ccgattactt gcgccatgat
     2761 ccctctcccc acgctcgaaa atgctggcgc ttcaagattg cgcccgttcg aacgcgttgc
     2821 ggagagaacg acaaagaaag cggcagctag gacagccgcg ccatccagcc gtgggtgtcg
     2881 gcgaaggtgc cgcgctgaat accggtcagc gtgtcgcgaa gggccatcgt gatttcgccg
     2941 ggctcgccgt cggcgatggt gaactcgccg tcggtgtgtt tcacatgcga caccggggtg
     3001 atcaccgcgg cggtgccgca cgcgaacacc tccgtgatct ctccggcggc cgccttcttc
     3061 tgccattcgt cgacgtcgat cttgcgctct tctacagcga aaccggcgtc ggtagccaac
     3121 tgcagcaacg agtctcgcgt gatgcctggc agcagcgaac cgctcagctc cggcgtgacg
     3181 aggcgggccg atcccccgct gccgaacacg aagaacaggt tcatgccgcc catctcctcg
     3241 acgtagcggc gttcgatggc gtccagccac accacttggt cacacccgtt gtcggcggct
     3301 tgggcctgcg ccagcagtga ggccgcgtag ttcccgccga acttcgccgc ccccgtgccg
     3361 cccggcgagg cgcgcacgta ctcgtgcgac aaccacacgc tgacgggttt gatgccgcga
     3421 gggaagtacg cccccgccgg tgaggcgatc accatgtagc ggtattcgga ggcggggcgc
     3481 accccgagtc ccggttcgga ggcgaagatg tagggccgca gatacagcga ctcctccccg
     3541 ccggcgggtg gcacccactg ctcgtcgacc gcgatgagct gtcgcagcga ctcgatgaac
     3601 acctcgtccg gcagctcggg gatggccagc ctgcgggccg acgagcgcag ccgtgcggcg
     3661 ttggcatcgg cccggaacga cacaacggag ccgtcggccc atcggtaggc cttcagcccc
     3721 tcgaacacct cctgcgcgta gtgcaggacg atggccgacg ggtcgagctc gatggggccg
     3781 tacgggatga ctcgcgcgtt gtgccaaccc ttgccctcga cgtaatcgat ggacaccatg
     3841 tggtcggtgt gaaagcgccc gaaccccgga ttcgacaaga tctcggcgcg tacctcgtcg
     3901 ctcgccggat tcgcattgcg atcgacggtg aactcgaggc ggtcagtcat gggccgattg
     3961 tataactgcg cgctaccggg tttttgcgtc gacgaacggc ggtttgacga cctcgcattc
     4021 gacgggcctg ccgcgcacgt cgaccgcgac gcgctggccg tcgctgacct cgtgatcggt
     4081 atcgagcaat gcgagtgcga tgcccacttt caaagtggga gagaaggttc ccgatgtcgt
     4141 cacaccgatt ggggtggtgc cgtcgagcac cgtcagctcc ggccgcagca ctcccctgcc
     4201 cacggcgcga agtccgcgca gca
//
LOCUS       DASYLX010000009         6605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3730946,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000009 DASYLX010000000
VERSION     DASYLX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3730946"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGP27_00205"
     CDS             <1..327
                     /locus_tag="VGP27_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEV7849771.1"
                     /translation="KVFGYDYQTVERSLNDIYPLLTPSYRQEFKDRANKDIIPQARER
                     QLVSQANTVGVGVLAAQRNSGSVMVYLNRTVTDKSKQPVYDGSRLKVDYVKVDGQWLI
                     NYITPI"
     gene            complement(314..1783)
                     /locus_tag="VGP27_00210"
     CDS             complement(314..1783)
                     /locus_tag="VGP27_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV7849772.1"
                     /translation="MSSGDDEEVGSGNSDFVVVANRLPIDMERLPDGTTRWKRSPGGL
                     VTALEPLLRRRKGAWIGWPGVPDADEEPIAEEDMRLYPVRLSAEDVEMYYEGFSNATL
                     WPLYHDVIVKPIYHRQWWDRYVEVNRRFAEATARAAAQGATVWVQDYQLQLVPKMLRM
                     LRPDLTIGFFLHIPFPPVELFMQMPWRTEIIEGLLGADLVGFHLPGGAQNFLILSRRL
                     VGANTSRASVGVRSRFGEVQIGFRTVKVGAFPISIDSGELDAKARARSVRQRAREIRN
                     ELGNPRKILLGVDRLDYTKGINVRLEAFSELLSEDRVKRDDTVLVQLATPSRERVESY
                     KVMREDIERQVGHINGEFGEVGHPIVHYIHRAVPRDELIAFFVAADVMLVTPLRDGMN
                     LVAKEYVACRSDLGGALVLSEFTGAAAELRQAYLTNPHHLDGVKDAIDEALNQSPEEG
                     RRRMRALRRQVLAHDVDRWARSFLEALASTRKGHDVRSG"
     gene            complement(1784..1945)
                     /locus_tag="VGP27_00215"
     CDS             complement(1784..1945)
                     /locus_tag="VGP27_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849773.1"
                     /translation="MATKSDPAELGDVEPLADSTARQARRVVAAYATDADECRVFLSM
                     LGIGPKLEA"
     gene            complement(1990..2382)
                     /locus_tag="VGP27_00220"
     CDS             complement(1990..2382)
                     /locus_tag="VGP27_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HEV7849774.1"
                     /translation="MATMKFSARRLIIAGGFAVAVAAAPAIAFVATPVPSAAPLACTG
                     GEEPDLYTSLCVPHLVPNAGQPYSTISGNPDLPAVNLPGGGGSIPCTGHNSGQCIGLA
                     EEQQAPVVTPESTIGSSPTVTGVEDNVG"
     gene            complement(2578..2934)
                     /locus_tag="VGP27_00225"
     CDS             complement(2578..2934)
                     /locus_tag="VGP27_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HEV7849775.1"
                     /translation="MATFHLPTRRLILAGGFAVAIAAAPAVAMFAVSPNGTSAPSVSA
                     CAGGETEDQFTNICVPDIVPNSPDFSSTAPVGGLPEIGGIPCTGHNSGQCIGLGEEEN
                     AAGPIAVPHSSVSSSP"
     gene            3287..4222
                     /locus_tag="VGP27_00230"
     CDS             3287..4222
                     /locus_tag="VGP27_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7849776.1"
                     /translation="MASPDLTNADSVPQEPVQQVTYETLDEGRIARIWLNRPGAQNAQ
                     SRTLLVQLDEAFGRAEADDNVRVVILAGRGKNFSAGHDLGSEEAMLERKPGPQQHPTF
                     RSHGSSMSSIAERTYLQEWHFFFENTRRWRDLRKITIAQVQGNAISAGLMLIWACDLI
                     VAADDAKFSDVVAVRMGMPGVEYYAHPWEFGARKAKELLLTGDSIDADEAYRLGMVSK
                     VFPRAELEDKTLAFARRIAERPTMAALLVKDSVNAASDAMGFTAALRHAFHIHELGHA
                     HWAAANENRFPVGLPPDVPDWRTLGAPTPSRRDTP"
     gene            4275..5114
                     /locus_tag="VGP27_00235"
     CDS             4275..5114
                     /locus_tag="VGP27_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849777.1"
                     /translation="MQLSLADRTVLVTGGGSGIGKGVARSAAEAGAKVMIVGRNADRL
                     AAAVDEIKPHVANGGSVLYEPADVTNEDEVSRVVEAATAWTGLLHGVVHCAGGSETIG
                     PVTQIDSEAWRRTVDLNINGTMYVLKHAAREMVRGGGGSFVGISSIAASNTHRWFGAY
                     GVSKSGIDHLMQLAADELGPSWVRVNCIRPGLIKTELVAPVLDSPELSADYASCTPLP
                     RPGEVEDIANAAVFLLSDASSYVTGQVINVDGGLMLRRGPDYSSMLEPVFGADGLRGV
                     VST"
     gene            complement(5111..6004)
                     /locus_tag="VGP27_00240"
     CDS             complement(5111..6004)
                     /locus_tag="VGP27_00240"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7849778.1"
                     /translation="MRIGFIGLGNMGAAMAANLVKAGHDVTVYNRSRAKVDALVAEGA
                     RPADSIADACGGDVVITMLANDDAVEAVTFGDGGILASLGRGATHVSSSTISVPLSER
                     MTAAHAEAAQQFVAAPVFGRPEAAQAAKLFVVAAGDRATLEAISGVLDAFSQRTFIVS
                     EEPKAANLVKLSGNFLITSVIESLGEAMALIGKAGVDKHQYLDVLTSTLFGAPIYKTY
                     GELIAGERFQPAGFAATLGQKDIRLVLAAAEDLQVPMPVASLIRDRFLTLLARGGADL
                     DWSAIGALPAWEAGASGPTGS"
     gene            6117..6572
                     /locus_tag="VGP27_00245"
     CDS             6117..6572
                     /locus_tag="VGP27_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV7849779.1"
                     /translation="MKFSTKFSTTFSTTLFQDGNNTGIEVPADVVDGLGSGKRPPVVV
                     NVNGFEYRSTVASMGGRYLLPFSAARRQESGIGGGDQIDVELTLDTAPRTVEVPADLR
                     GALDSSPTAAAAWETLSTSNRRAQVESVLGAKAAQTRARRIAAIVAKLG"
BASE COUNT         1143 a         2108 c         2199 g         1155 t
ORIGIN      
        1 aaggtgttcg gctacgacta ccagactgtc gagcgcagcc tcaacgacat ctatccgctt
       61 ctgacgccga gctaccgcca ggagttcaag gaccgggcga acaaggacat catcccgcag
      121 gcgcgtgaac ggcaactggt cagccaagcc aacaccgtcg gagtgggagt gctggccgcg
      181 caacgcaatt caggctcggt catggtgtat ctgaaccgca ccgtgaccga caagtccaag
      241 cagccggtct atgacggcag tcggctgaag gtggactacg tcaaggtcga cggccagtgg
      301 ctgatcaact acatcacccc gatctgacgt cgtgaccttt gcgagtggag gccagcgcct
      361 ccaggaacga ccgcgcccag cggtcgacgt cgtgggcgag cacctgcctg cgtagggcgc
      421 gcatcctgcg tctgccctct tccggcgact gattcagcgc ttcgtcgatc gcgtccttca
      481 cgccgtcgag gtggtgaggg ttggtcaggt atgcctgccg gagttcggcc gccgcgccgg
      541 tgaattcact gaggaccagg gcgccgccca aatcgctgcg gcaggcgacg tactccttgg
      601 cgaccaggtt catgccgtcg cgcaacgggg tcaccagcat gacgtcggcc gccacgaaga
      661 aggcgatcag ttcgtcgcgg gggaccgccc ggtgaatgta atgcacgatc gggtgtccga
      721 cctcaccgaa ttcaccgttg atgtggccga cttgccgctc gatgtcctcg cgcatcactt
      781 tgtagctttc cacgcgctca cggctcggcg tggccagctg cacgagcact gtgtcgtcgc
      841 gtttgacgcg gtcctcgctg agcagttctg aaaacgcttc cagccgaacg tttatgccct
      901 tggtatagtc cagccggtcg acgccgagca ggatcttgcg cggatttccg agctcgttgc
      961 ggatctcgcg cgcacgctgg cgaacgcttc tcgccctggc cttggcatcg agttcccccg
     1021 agtcgatcga gatggggaaa gcgccgactt tgactgtgcg aaagccgatc tgcacctcgc
     1081 cgaatcggga ccggacaccg accgaggctc gtgacgtgtt ggcgcccacc aggcgtcggg
     1141 acaggatcag aaagttctgg gcgccgcccg gcagatggaa gccgaccagg tccgcaccca
     1201 gcaggccctc gatgatctcg gtgcgccacg gcatctgcat gaacagctcc accggcggaa
     1261 acggaatgtg caagaaaaat ccgatggtga gatcgggccg cagcatccgc aacatcttcg
     1321 gtaccagctg cagctggtag tcctggaccc acacggtcgc gccctgcgcg gcggcgcgcg
     1381 cggtcgcctc ggcgaagcgc cgattcacct cgacgtagcg atcccaccac tggcggtggt
     1441 agatcggctt gacgatgaca tcgtggtaca gcggccacag cgtggcgttg gagaacccct
     1501 cgtagtacat ctcgacgtct tcggccgaca gccgcaccgg atagagccgc atgtcttcct
     1561 cggcgatcgg ctcctcgtcg gcgtccggaa cacccggcca gccgatccag gcgcccttcc
     1621 gtcggcgcaa cagcggctcg agggccgtga caaggccgcc agggctgcgt ttccagcgcg
     1681 tcgtgccgtc aggcagccgc tccatgtcga tcggcagccg gttggccacg acgacgaagt
     1741 ccgaattgcc ggagccgact tcctcgtcgt ctcccgaact cacctacgcc tcgagcttcg
     1801 gtccaatgcc cagcatcgac agaaagaccc ggcactcgtc tgcgtcggtc gcataggcgg
     1861 cgacgacgcg gcgggcctgg cgggcggtgc tgtcggctag cggttcgacg tcgccgagct
     1921 cggcgggatc agattttgta gccatcacac caccatatat gcggccggac ggatgagcgc
     1981 cccgacgggc tacccgacgt tgtcttcgac accggtgact gtcgggctgc tgcctatcgt
     2041 cgactccggt gtaacgacgg gtgcctgctg ttcctctgcc agcccgatgc actgtccgga
     2101 gttgtggccg gtgcagggaa tggacccgcc gccgccgggc aggttcaccg cgggcaggtc
     2161 ggggttgccg ctgatcgtcg aatacggctg gccggcattc ggcaccaggt gcggaacgca
     2221 cagcgaggtg tagaggtcgg gctcctcgcc accggtacag gccagcgggg cggccgaggg
     2281 gacgggtgtc gcgacgaagg cgatcgccgg cgctgccgcg accgcgacgg cgaatccacc
     2341 ggcaatgatc agtcgtcgtg ctgagaactt catggtcgcc atcgtcacgc tttctgtaga
     2401 actcctgacg gttatcgtcg gggcgattgt atggaaactg ggcgatatct gcatgagttc
     2461 gcaaagtttc ctgtcaagac gctcgcgcga tttctgcagg ccaacccgag aaacgtggcg
     2521 cacaaaggaa agggccggac gccaaaatgg cgtccggccc tacctgatct ggctcttcta
     2581 cgggcttgag ctgaccgagg aatgtggaac cgcgatcggg cccgcggcat tctcttcctc
     2641 gcccaggccg atgcactggc ccgaattgtg tccggtgcag gggatgccgc cgatctccgg
     2701 taggccgccg accggcgcgg tgctcgagaa gtccggtgag ttcggcacga tatcgggcac
     2761 gcagatattg gtgaactggt cctcggtttc accgcctgcg catgccgaaa ccgacggcgc
     2821 cgacgtgccg ttcggcgaaa cggcgaacat tgcgaccgcc ggtgcagcag cgatcgcaac
     2881 agcgaatccc cccgcaagga tcaggcgtcg agttggaagg tggaaggtcg ccatcgtcac
     2941 gctttctgta gtttgctggc cgtgtcggtc gagaagcggc tggttgctgt cagcgccctg
     3001 accgcaaacc tccggttccc tgcctatgcc tcgtccgaag ttgtagcagg agttggcatc
     3061 tgctagcgca agcgtacctg cgactaacga aaagtactgg ctgataaccg gtatccgcgg
     3121 tttgctcgtc gtctaactgt gaggtgactg ccaggttgct gtggtgacgc tgcgcagacg
     3181 gctggggccc aaggtcgtcg cggcggacat cccgccgggc gacgggttgg gcaggagaag
     3241 tagtgtgcct tacatctgaa gcgtcgaacg taacgaggtg tgcaccgtgg ctagcccgga
     3301 cctgaccaac gccgattcgg ttccgcagga gccggtccag caggtgacct acgagacctt
     3361 ggacgagggg cgaatcgccc gtatctggct caaccggccg ggcgcccaga atgcgcaatc
     3421 gcgcaccctg ctcgtgcaat tggatgaggc attcggcagg gccgaggccg atgacaacgt
     3481 gcgggtggtg attctggccg gtcgcggaaa gaacttctcc gccgggcacg atctcggctc
     3541 agaggaggcc atgctcgaac gcaagccggg accgcagcag cacccgacgt ttcgctcgca
     3601 cggctcgagc atgagcagca tcgccgagag gacgtatctg caggaatggc acttcttctt
     3661 cgaaaacacc cgacgctggc gggatctgcg aaagatcacc atcgcgcagg tgcagggaaa
     3721 tgccatttcc gccggactga tgctgatctg ggcgtgtgac ctcatcgtgg ccgccgacga
     3781 tgcgaagttc agcgacgtcg tggcggtccg gatgggtatg ccgggtgtcg agtactacgc
     3841 gcatccatgg gaattcggcg cgcgcaaggc caaagagctt ctgctgaccg gtgattcgat
     3901 cgacgcggac gaggcatacc gtctcggcat ggtgtccaag gtcttccctc gcgccgagtt
     3961 ggaggacaag acgttggcgt tcgcgcgtcg tatcgccgag cggccgacga tggcggccct
     4021 acttgtgaag gattcggtca acgcggccag cgacgccatg ggcttcaccg cggcattgcg
     4081 gcacgcgttt cacattcacg agctcggcca cgcgcattgg gcggcggcca acgagaacag
     4141 gttccccgtc ggtctgccgc ccgacgttcc ggactggcgc acgctgggcg ccccgacgcc
     4201 atcgcgtcgt gacacccctt gacgtgcgtg gttccataac gagaacctgt tccagttttt
     4261 cgtgaggagt gacggtgcag ctgtcgttgg cggataggac ggtcctggtc accggcggcg
     4321 gcagcggaat aggcaagggc gtcgccaggt cggccgccga agccggcgcg aaggtcatga
     4381 tcgtcggccg taacgccgac cgacttgccg ccgccgtcga cgagatcaaa ccgcatgtcg
     4441 ccaacggggg ctcggtgctc tatgaacccg ccgacgtcac caacgaagac gaggtcagcc
     4501 gggtcgtcga ggcggcgacc gcgtggaccg gcttgctgca cggggtggtg cactgtgcag
     4561 gcggaagcga gacgatcggt cccgtcacgc agatcgactc cgaggcgtgg cggcgcaccg
     4621 tcgacctgaa catcaacggc accatgtacg tgctcaagca cgcggcgcgg gaaatggtcc
     4681 ggggcggcgg cgggtccttc gtcgggatct cgtcgatcgc agcgagcaat acccatcggt
     4741 ggttcggcgc atacggcgtc tccaagtccg gtatcgacca tctgatgcag ttggccgccg
     4801 acgagctcgg cccgtcgtgg gtccgggtga actgcatccg cccgggcctt atcaagaccg
     4861 aactcgttgc gccggttctg gattcgcccg aactgagtgc tgactacgca tcgtgcacgc
     4921 cgctgccgcg gcctggcgag gtcgaggaca tcgccaacgc cgccgtcttc ctgctcagcg
     4981 acgcgtccag ttacgtcacc ggtcaggtga tcaacgtcga cggaggcctt atgctgcggc
     5041 gcggtccgga ctactcctcg atgctcgagc ccgtcttcgg cgccgacggg ttgcgtggcg
     5101 tggtgtcgac ctaggaaccg gtgggaccgg aggcacccgc ctcccaggca ggcagagcgc
     5161 cgattgccga ccagtcgagg tcggcgccgc cgcgggccag cagggtcagg aagcgatcgc
     5221 ggatcaagct ggccaccggc atcggcacct gtaggtcttc cgcggcggcc aacacgagcc
     5281 ggatgtcctt ctgcccgagc gttgcggcga atccggcggg ttggaatctc tcacccgcga
     5341 tcagctcgcc gtatgtcttg tagatcgggg cgccgaacag cgtcgaggtg aggacgtcga
     5401 ggtactggtg cttatcgacg ccggccttgc cgatcagggc catcgcctcg cccagcgact
     5461 cgatcaccga tgtgatgagg aaattgccgc tcaacttcac caggttggcg gctttcggct
     5521 cttcggagac gatgaacgtg cgctggctga aagcgtcgag cactcccgaa atcgcttcca
     5581 gcgttgcgcg atcgcctgcc gcgacgacga agagcttcgc cgcctgcgcc gcctccggcc
     5641 tgccgaatac cggcgcggcg acgaactgtt gggccgcctc ggcgtgggcg gcggtcatcc
     5701 gttcggagag cggcacgctg atggtcgacg acgacacgtg agtcgcaccg cggccaagcg
     5761 aggcgagtat tccgccgtcc ccgaaggtga ccgcctcgac ggcgtcgtcg ttcgcgagca
     5821 tggtgatcac gacgtcaccg ccgcacgcgt cggcgatcga gtccgccggc cgtgcgccct
     5881 cggcgacgag ggcgtcgacc ttggcccgcg accggttgta gaccgtgacg tcatggcccg
     5941 ccttgacgag gttcgcggcc atggccgctc ccatgttgcc cagtccgatg aagccgatcc
     6001 gcagtttgtc cctggccatg cgccgagtat gcgcccgtca cccggctctc gaatcgaccc
     6061 gagccaaatc cgcagggcac agcgaatttt cgtcagttct gtgctatgcc ggatgcgtga
     6121 agttctcgac caagttctcg accacgttct cgacgacgtt gttccaggac ggcaacaaca
     6181 ccggcatcga agtcccggcc gatgtggtgg acggtctcgg gtcgggtaag cggccgcccg
     6241 tggtggtgaa tgtgaacggc ttcgagtacc gctcgacggt tgcctccatg ggcggcaggt
     6301 atctgcttcc gttcagcgcg gccaggcggc aggagtcggg gatcggggga ggtgaccaga
     6361 tcgacgtcga gttgacgctc gacaccgcgc cgcggaccgt cgaggtgccc gcggacctac
     6421 gtggggcgct ggactcttcg cctaccgcgg cggccgcctg ggagacgctc tcgaccagca
     6481 accgcagagc gcaggtcgaa tcggtgttgg gcgccaaggc cgcccaaacc cgcgcccggc
     6541 gcatcgcagc cattgtcgcc aagctggggt aggcccattg cgtgcgaaat gggtcatggc
     6601 gccct
//
LOCUS       DASYLX010000010         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3621212,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000010 DASYLX010000000
VERSION     DASYLX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621212"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VGP27_00250"
     CDS             <1..885
                     /locus_tag="VGP27_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849780.1"
                     /translation="KATLGLTTIVVVIAQLIGLALQIGPLAAMGELAVLRGEEASRAA
                     ELGSSASGIAGTITTLLSSIVLSGMLTVIVGRAVFGAGITIGEAWRRVRGRLLPLFGY
                     TLLWLLAVAVAVLVVVFVVVAVASLSGAVAFVLAIPLGLGLIALLVYLWTVLSFAPPL
                     IVLERLGVVPSVKRSIRLVKGDFWRVLGIRLLATLVAGVVAAAVSIPFSLTGQILLMS
                     SEATTMTLIALALIAIGGAVGQIITAPFSAGAVVLLYTDRRIRAEAFDLVLQTGAAIA
                     APGAAPDSTDHLWLTRQP"
     gene            887..1540
                     /locus_tag="VGP27_00255"
     CDS             887..1540
                     /locus_tag="VGP27_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV7849781.1"
                     /translation="MPAIDIDRDAAREAAQHELAKPIYPKPSLTERFAEWLDDLLYRI
                     AQEGSSLPGGWFTISVLLIVLAVAIVVAVRIARRTMRTDRGGEHALFDSHELTAAAHR
                     ATAQQYAAQGNWTAAIRHRLRAVARQLEEDGVLNPVPGRTATELAKDGGAVLPGLLSE
                     LLQAATTFNDVSYGQRPGTEAAYRMIADLDDHLRSRGPARITAPVAGGPAESWTAVR"
     gene            1537..2679
                     /locus_tag="VGP27_00260"
     CDS             1537..2679
                     /locus_tag="VGP27_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEV7849782.1"
                     /translation="MTPVETAVTPTARQHWRTGRWVLSALLVVGAVFTVSALLTAPRP
                     GGRMDPAATSPEGAHALVTLMRDRGVDVVVADDIAAVERAARPDSLVLTVQTDHLVDD
                     DVLRRLASVPGDRLVVEPLSRTREVLAPEVRRAAAGTVGGSPDCDLREATRAGDVQLG
                     SSDTFEPSGDVPVTRCYEGAVVRYTDEGRTVTVVGGADFMTNSGLPKQGNAALAMNLA
                     GTQSRVIWYAPQHAEGESSGGASLSDLIPERVTWVVWQLGLVVLLLALWKVRRIGPLV
                     AEHLPVVVRASETVEGRGRLYRSHRAGDRAADALRTATLQRLVPRLGLSADADADAVV
                     QTVAQRTGRDPQMVGHALFGPPPATDADLVKLAHMLDDIERQVAQS"
     gene            2676..3665
                     /locus_tag="VGP27_00265"
     CDS             2676..3665
                     /locus_tag="VGP27_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7849783.1"
                     /translation="MTQPVAETSGAATATSDSARNALLALRNEIAKVVVGQDAVVSGL
                     VIALLCRGHVLLEGVPGVAKTLLVRTLAAALQLEFKRVQFTPDLMPGDVTGSLVYDAR
                     TAEFEFHAGPVFTNLLLADEINRTPPKTQAALLEAMEERQVSVEGDPRPLPDPFIVAA
                     TQNPIEYEGTYQLPEAQLDRFLLKLNVPLPPRDQEIAILIRHARGFDPRDLSSVQPVA
                     GANELAAGRAAVREVLVADEVLGYIVDIAGATRHSPSLQLGVSPRGATALLATARSWA
                     WLSGRNYVTPDDVKAMARPTLRHRIALRPEAELEGASPDAVLDGILAAVPVPR"
     gene            3665..>4147
                     /locus_tag="VGP27_00270"
     CDS             3665..>4147
                     /locus_tag="VGP27_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7849784.1"
                     /translation="MVLTGRAGMVALMCVLPIALSPWPATAFFILLAVLAIAMAVDVS
                     LAAGTRQLQFTRSGDTAARLGQPVAATLSIVNAGRRRLRGLVRDAWPPSAGAQPRTHP
                     VDLAAGQQIQVETWLRPVRRGDQLSALVTARSLGPLGLAGRQSSHRVPWQVRILPPFL
                     S"
BASE COUNT          594 a         1443 c         1416 g          694 t
ORIGIN      
        1 aaggcgacgc tcgggctgac gacgatcgtc gtggtgatcg ctcagctgat cgggctggca
       61 ctgcagatcg ggccgctggc cgccatgggc gaactcgccg tgctccgcgg tgaggaggcg
      121 tcgagggccg cggagctggg gtcatccgcg tcgggcatcg ccggcacgat caccacactg
      181 ctgtcgtcga tcgtgctcag cggcatgctg acggtcatcg tgggcagagc ggtcttcggc
      241 gcgggcatca cgatcggtga ggcgtggcga cgggtccgcg ggcggctgct gcccctgttc
      301 ggctacacgc tgctgtggct tctcgcggtc gccgttgccg tcctcgtcgt ggtgttcgtc
      361 gtcgtcgccg tcgcatcgct cagcggcgcc gtggcgttcg tcctcgccat cccgctggga
      421 ctcgggttga tcgcgttgct cgtctatctg tggacggtgc tcagcttcgc tcccccgctg
      481 atcgtgctgg agcgcctcgg tgtcgtccct tcggtcaagc ggtcgatcag gttggtgaag
      541 ggtgacttct ggcgggtact ggggatccgg ctgctggcga cgctggtcgc aggcgtcgtc
      601 gccgcggcgg tgtcgatacc tttcagcttg accggccaaa ttcttctgat gagttcggaa
      661 gccaccacga tgacgttgat cgcgctggcg ctcatcgcca tcggcggcgc ggtcggccag
      721 atcatcacgg cgccgttcag tgcgggcgcg gtggtgctgc tctacaccga tcggcggatc
      781 cgtgccgagg cgttcgacct ggtgctgcag accggcgcgg cgatcgccgc accgggtgcg
      841 gcgcccgact cgaccgacca cctctggctc acccggcagc cctgacgtgc ccgcgatcga
      901 catcgaccga gacgccgcgc gggaggcggc gcagcacgag ctcgccaagc cgatctatcc
      961 caaaccgtct ctgaccgagc gctttgcgga atggctcgac gacctgctct accgcatcgc
     1021 ccaagagggc tcctcgctgc ccggcggctg gttcaccatc tccgtcctgc tcatcgtcct
     1081 cgccgttgcc atcgtggtcg ctgtgcgaat cgcccgacgg acgatgcgca ccgaccgcgg
     1141 cggcgaacac gcactcttcg attcacacga gctgaccgcc gctgcgcatc gggccacggc
     1201 gcaacagtat gcggcacagg gcaattggac tgcggccatc cgccaccggc tcagggccgt
     1261 cgcgcgtcaa ctcgaggaag acggggtgct caacccggtg ccgggccgca cagcgaccga
     1321 gctcgccaag gacggcggcg cggtgctgcc gggcctgttg agcgaattgc tgcaggccgc
     1381 aaccaccttc aatgacgtca gctacggaca gcggcccggg acggaggccg cctaccgcat
     1441 gatcgccgat ctcgacgacc atctgcgctc acgtgggccc gcgagaatca ccgcacctgt
     1501 ggctggcggc ccggctgaga gctggacggc cgtgcgatga cgcctgtcga aacggccgtc
     1561 acgccaacgg ccaggcagca ctggcgtacc ggccgctggg tgctgtcggc cttactggtc
     1621 gtcggagccg tcttcacggt cagcgccctg ctgactgcgc ctcggcccgg cggccggatg
     1681 gatcccgccg caacgtctcc cgaaggtgcg cacgccctcg tcaccctgat gcgcgaccgc
     1741 ggcgtcgacg tggttgtcgc cgacgacatc gccgctgtgg aacgagcggc acgaccagac
     1801 tcgttggtgc tgacggtgca gaccgatcac ctcgttgacg acgacgtgct gcggcggctg
     1861 gcctccgtgc ccggcgaccg gctcgtggtc gagcccttgt cgcgcacgag ggaggtcctc
     1921 gcgccggagg tccgacgggc cgcggcgggc accgtcggcg gctcgccgga ttgcgacctg
     1981 cgcgaagcca ccagggccgg agatgtgcag ctcggctcga gcgacacctt cgagccgtcc
     2041 ggcgatgtcc cggtgacccg atgctacgag ggtgccgtgg tccgctacac cgacgaaggc
     2101 cgcaccgtga cggtcgtcgg cggcgcggac ttcatgacca actcgggcct gcccaaacag
     2161 ggcaatgctg cgctggcgat gaacctcgcc gggacgcaat cgcgagtgat ctggtatgcg
     2221 ccgcagcacg cggagggcga atcgtccggc ggcgcatcgc tttccgatct gataccggaa
     2281 cgcgtgacgt gggtcgtctg gcagctgggc ctggtcgtgc tgttgctcgc gctgtggaag
     2341 gtccgacgca tcggtccgct cgtcgccgaa cacctgccgg tggtcgtgcg cgcctccgag
     2401 acggtcgagg gccgcggccg gctctaccgg tcccatcgtg ccggtgaccg cgccgccgat
     2461 gcgctgcgca ccgcaaccct gcaacgactc gttccccggc tcggcctcag cgccgacgcc
     2521 gacgccgacg ccgtcgtcca aacagtcgct cagcgcaccg gccgagatcc acagatggtc
     2581 ggacacgcgc ttttcggccc gccgccggcc accgacgccg acctggtaaa gcttgcccac
     2641 atgctcgacg acatcgaaag gcaggtcgca cagtcgtgac tcagccagta gccgaaacct
     2701 ccggagccgc aacggcgaca tcggattcag cgcgaaacgc gttgttggcg cttcgcaacg
     2761 aaatcgccaa ggtggtcgtc ggccaggacg ccgtcgtcag cggcctggta atcgcgctgc
     2821 tgtgccgcgg ccatgtgctc ctcgagggtg tacccggcgt cgccaagaca ctgttggtgc
     2881 gaacgctcgc cgccgcactg caattggagt tcaagcgcgt ccagttcacc cccgatctga
     2941 tgccggggga cgtcaccggc tcactggtgt acgacgcgcg caccgccgaa ttcgaattcc
     3001 acgcgggccc ggtcttcacc aacctgctgc tggccgacga gatcaaccgg acaccgccga
     3061 agacccaggc ggcgctgctc gaagcgatgg aggaacgtca ggtcagtgtc gagggcgatc
     3121 cacgtccgct gcccgatccg ttcatcgtcg ccgccaccca gaacccgatc gagtacgagg
     3181 gcacctacca gctgccggaa gcacaactgg accggttcct gctgaagctc aacgtgcctc
     3241 tgccaccgcg cgatcaggag atcgcgatcc tgatccggca tgcgcgcggc ttcgatccgc
     3301 gggacctctc ctcggtgcag cccgtcgcgg gtgcgaatga actcgccgcg ggacgtgcgg
     3361 cggtacgcga ggttttggtg gcagacgaag tgctcggcta catcgtcgac atcgcaggtg
     3421 ccacccggca ttcaccgtcg ctgcaactag gtgtctcgcc gcgcggcgcc acggcactgc
     3481 tggcgactgc tcggtcgtgg gcgtggctgt cgggccgcaa ctacgtcacc cccgacgacg
     3541 tcaaggcgat ggcgcggccg accctgcgcc atcggatcgc gttgcggccg gaagccgaac
     3601 tcgagggcgc cagccccgac gctgtgctcg acggcatcct ggctgccgtg ccggtgcccc
     3661 gatagtggtt ctcaccggcc gcgccgggat ggtggcgctg atgtgcgtgc tgccgatcgc
     3721 tctgtcacct tggcctgcaa cggctttctt catcctgctc gcggtgctgg cgatcgcgat
     3781 ggcggtggat gtgtcgttgg ccgccggcac ccgtcagctg cagttcacca ggtccggtga
     3841 caccgcagcc cggctgggtc agccggtcgc cgccacgctg tcgatagtga atgcggggcg
     3901 gcgtcgcctg cgcgggctgg tccgcgacgc ctggcctccg agtgcaggcg cgcagccccg
     3961 cacacatcca gtggatttgg cggcgggcca acagattcag gtagagacct ggttgcggcc
     4021 ggtgcgtcgc ggcgaccagc tctcggcgtt ggtcaccgcc cgctcgctgg gtccgctcgg
     4081 ccttgcgggc aggcagagct cgcaccgggt gccatggcag gtccgtatcc tgccgccgtt
     4141 cctgtcg
//
LOCUS       DASYLX010000011         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1866122,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000011 DASYLX010000000
VERSION     DASYLX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866122"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..360)
                     /locus_tag="VGP27_00275"
     CDS             complement(136..360)
                     /locus_tag="VGP27_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849785.1"
                     /translation="MPSTLPIAQHHFRRVAGTALGEWSTRTCEAALARRGLRADTSNL
                     PHDTIVVPETGICAAAYAAGMVGARRAGMR"
     gene            579..803
                     /locus_tag="VGP27_00280"
                     /pseudo
     CDS             579..803
                     /locus_tag="VGP27_00280"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1104..2234
                     /locus_tag="VGP27_00285"
     CDS             1104..2234
                     /locus_tag="VGP27_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEV7849786.1"
                     /translation="MEAAAAQRAAPRPKAQRALVGVFLLALTCAGGYAVAAHKTVTLV
                     VDGSPMTTSTMTSRVIDVVRDNGFAVGDHDDLYPAASQTIHQSDTIVLRRGRPLQVSV
                     DGQGRRQVWTTALTVGDALKQLSMSDAAPAAASRASRLPLAGMALPVVSAKNAHIIDG
                     GVASDRRLAALNVGMLLAAADAPLEQDDKVVPPASTPVTEGMQIAVTRIRTQKVTARM
                     PLPPSTRRIADPAMNMSRYVVEDPGVPGTQDVTFAVSMVNGVETGRRLTAHDIVIPAR
                     PSVLRIGAKPDTMVPPVSNGATWDALAACEAGGNWAINTGNGFYGGVQFDQNTWNRHG
                     GLRYAPRADLATREEQLAIAEVTLARQGWGAWPVCSVKVGAK"
     gene            2288..2980
                     /locus_tag="VGP27_00290"
     CDS             2288..2980
                     /locus_tag="VGP27_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7849787.1"
                     /translation="MRQPTAGLALASVCLLTVGCSSGSNQGTETSTTRSTTTTTTAPP
                     LPDTALPGLLLGDEQVNAAMGATEMAVTRTHFAMSDDSTTMEPRECLAIDGSAQAVVY
                     ADSGFTAERDQTLQEGDNFTHYVQQAVVLFPSAKQAVAFLSASAQQWPACHHYTHTQS
                     GSLWDVGPISNEDGTLSTIATQQNANDPGWACGRALVQRNNVLVDVNTCSANPADSAL
                     NIANQIAAKVPT"
BASE COUNT          550 a          975 c         1029 g          488 t
ORIGIN      
        1 cggctcctag ctcacggtcc tgacgcgccg cttgtcgagt gagtcacgcc aacggtgatt
       61 cggagctgtc gcgcatctga catctcaacc tgctgccggg gactcgcctg cacgagctaa
      121 ccaaaatggt cccgatcaac gcatgcctgc ccggcgtgca ccgaccatcc cggcggcgta
      181 ggcggcggcg cagattccag tctcgggcac cacgatggtg tcgtgcggca ggttcgaggt
      241 atcggcccgc agaccccgtc gcgccagggc cgcttcacat gttctggttg accattctcc
      301 gagcgcagtg ccggcgacac gccggaagtg gtgttgggct atgggcagtg tggacggcat
      361 gataagaggc tcatgagctc ggttgtggtg ggttgtccga ggtagctctc gggcagagcg
      421 aagacacgca cttcgggagg ccgaagtcgc gtggcgcgca gcacttcccc gtgttgagct
      481 cgggtcaggg atcgttgccg ccatcgctaa acgactggct gccagccaag cacacggccc
      541 ggttgatcgc cgaattggtc gacgcgcatg tggatctggt gcctatccgg gccgcggatg
      601 atgcgggacg cgccgcgacg ccccatgatc cgcggctgat ggtgcgaatc ctgttgtacg
      661 cgtacaccac cggggtgagt tcgtcgcggg tgatcgaacg caaatgtgtc gatgatgttc
      721 cctttcggtg gttggccgat ggggcggcgc cggactatcg ggcgatcgca ggatttcgta
      781 agcggcacgc gtctgcgctc gggaacctgt tcgtggaggc gttggcgttg tgccgggcgg
      841 cgggcatagt gcggctgggg cgtttcgtgc tggacccgtc gagggtgcga gacagcgcgt
      901 cgacacgcca ggtgatgcgc aaatccaggg atgagacgga aagggttctc gccgaggggg
      961 tctggaccct acggacagag gccgaatcga tagatgaggc cgaggatgcg acgtcgggcc
     1021 aaaacggtcg cggtgacgag tcaccaaagc gcttgcggcg aaccgaaact cggcggccca
     1081 agatcggcgg gggcaatggc cgcctggagg ccgcggccgc ccagcgggcc gctccgagac
     1141 cgaaggcgca gcgcgcgctg gtcggcgtgt tcttgttggc gttgacctgc gccggcggct
     1201 acgccgtcgc agcgcacaag accgtcacgc tcgtcgtcga tggctcaccg atgacgacgt
     1261 cgacgatgac gtcgcgtgtg atcgatgtgg tgcgggacaa cggcttcgcc gtcggtgacc
     1321 acgacgatct ctatccggcc gctagtcaga caatccacca gtccgacacc atcgtgctgc
     1381 gacgcggccg gccgctgcag gtgtcggtgg acggccaagg gcgcaggcag gtgtggacca
     1441 cggctttgac tgtcggcgac gctctcaagc agctgtctat gagcgacgcg gcaccggctg
     1501 cggcgtctcg cgcgagccga ttgccgttgg ccggcatggc gctgccggtg gtcagcgcga
     1561 agaacgcgca catcatcgac ggcggtgtcg ccagcgaccg gcgcctggcg gcgctcaacg
     1621 taggaatgct gctggccgca gccgacgcgc cgttggaaca ggacgacaag gtggtgccgc
     1681 cggcgtcgac gccggtcacc gagggcatgc agatcgcggt cacccgcatc cggacgcaga
     1741 aggtcaccgc gcgcatgccg ctgccgccat cgacacgacg catcgcggac cccgcgatga
     1801 acatgagccg ctacgttgtc gaggatcccg gcgttcccgg cacccaggac gtcaccttcg
     1861 cagtgtcgat ggtcaacggc gtcgaaaccg gccggcggct gacggctcac gacatcgtga
     1921 tacctgcccg gccctcggtg ctgcggatcg gcgccaagcc ggacaccatg gtgccgcccg
     1981 tgagcaacgg cgcgacctgg gatgcgctgg ccgcatgtga ggccggaggc aactgggcca
     2041 tcaacaccgg caacggcttc tatggcggcg tgcagttcga ccagaacacc tggaaccgcc
     2101 acggcggact gcgttatgcg ccgcgcgccg acctggccac ccgagaagag caactcgcga
     2161 tcgccgaggt cactctggca cggcagggct ggggcgcctg gccggtatgc agcgtcaaag
     2221 tgggggcgaa gtgatgatca gctgtgctga tcggctgatg gttacggcag ggaaccggga
     2281 tgaacacgtg cgccaaccaa ccgccggact cgccctcgcc agcgtctgcc ttctcaccgt
     2341 cggatgcagc agcggcagca accaaggcac cgaaacatcg accaccaggt cgacgaccac
     2401 cacgaccact gcgcctcctc ttcccgacac cgcgctgccg gggttgctgc tcggcgacga
     2461 acaggtgaac gccgccatgg gggcaaccga aatggcggtc acgagaaccc acttcgcgat
     2521 gtctgacgac agcaccacga tggagcccag ggagtgcctc gccatcgacg ggtcggcgca
     2581 ggcggtggtc tatgccgaca gcggtttcac ggccgagcga gaccagacat tgcaagaggg
     2641 cgataatttc actcactatg tccagcaggc agtggtgttg tttccctccg ccaaacaggc
     2701 ggtcgcgttc ctttctgcct ctgcccagca gtggccggct tgccaccact acacccacac
     2761 ccagtccggc tcgctgtggg acgtcgggcc gatatccaac gaggacggca cactgagcac
     2821 cattgcaact cagcagaacg ccaacgaccc cgggtgggcc tgtgggcgag cactggtgca
     2881 acgaaacaac gtccttgtcg acgtcaacac atgcagcgcc aaccctgcgg actcggcgct
     2941 caacatcgcc aaccagatcg ccgccaaagt gcccacttga tgtgccaccg gcaccgcact
     3001 accgtcggat gagttcagtc cgcttagtga aataagctgg ta
//
LOCUS       DASYLX010000012         6933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2305137,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000012 DASYLX010000000
VERSION     DASYLX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305137"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VGP27_00295"
     CDS             complement(<1..354)
                     /locus_tag="VGP27_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041310272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV7849788.1"
                     /translation="MPDDPASQPPFSRAESIVNHAEQSISEDAIVAAARDRAVETGAG
                     AVTPAVGALLSVLAKLSGAKTVVEVGTGAGVSGLWLLSGMREDGVLTTIDVEPEHQRL
                     AKQAFNEAGIGPSRTR"
     gene            544..1308
                     /gene="sigE"
                     /locus_tag="VGP27_00300"
     CDS             544..1308
                     /gene="sigE"
                     /locus_tag="VGP27_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HEV7849789.1"
                     /translation="MEHGGRWRSGNNHVDRHVVADVALPTSGERCDLEDSTSTTTTAA
                     HLAPVSMPHLERFTDGEWVEPSEEPQGTAVFDATGDHSTMPSWDELVRQHADRVYRLA
                     YRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRGRIRME
                     ALPEDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIG
                     ATLGVKLGTVRSRIHRGRQALRDHLASHSADTARSA"
     gene            1398..1793
                     /gene="rseA"
                     /locus_tag="VGP27_00305"
     CDS             1398..1793
                     /gene="rseA"
                     /locus_tag="VGP27_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma E factor RseA"
                     /protein_id="HEV7849790.1"
                     /translation="MADPGHVFRRAFSWLPTQFASQSDSPVGPRQFGSTEHLSTEAVA
                     AFADGELRMTAHLRAAHHLSLCTQCAAEVEAQRQARAALRDSRPIAIPTSLLGLLAQI
                     PQDTAQVAPDENEQTQFADDTIRGRRKRR"
     gene            1863..3356
                     /locus_tag="VGP27_00310"
     CDS             1863..3356
                     /locus_tag="VGP27_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7849791.1"
                     /translation="MTKQDQTDGSHRVEPRPVSRPPVDPSTTRAFGRPDGVNGSFNGA
                     ETKPDQGEFTPTDQPPDPVLHEAFGRPNGRGDSLQRHPADIGALDAERDRQKPQELDD
                     PWRDPGAAAALGTPAVPTPAAHPAATNVGKLGVRDVLFGGRVSYVALAVLAIVALVIG
                     FAGGWVGRKTAEVVEAFTTSKVTLSTDTNSEQPQGRFAKVAASVADSVVTIEAVGDNE
                     GAQGSGVVVDPRGYIVTNNHVISEAATNPSQYKLSVVFNDGKSVPANLVGRDPKTDLA
                     VLKVDNVDNLTVAKLGDSDELHVGDEVIAAGAPLGLRSTVTQGIISALHRPVPLSGDG
                     SDTDTVIDGVQTDAAINHGNSGGPLINMNSDIIGINTAGKSLSDSASGLGFAIPVNEV
                     KNVVEALIKDGKIAHPTLGLTARSVSNDLASGAQVANVKAGGPAEKAGILENDVVVKI
                     GDRTVADADEFVVAVRQLKIGQDAPVEVVRDGRHVTLTVNPGSDNST"
     gene            3356..3757
                     /gene="tatB"
                     /locus_tag="VGP27_00315"
     CDS             3356..3757
                     /gene="tatB"
                     /locus_tag="VGP27_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEV7849792.1"
                     /translation="MFANVGWGEMLVLLIAGLVILGPERLPGAIRWTSDAMRQARDYI
                     SGATKQLRDDLGPEFDDLREPLSELQKLRGMTPRAALTKHLLDGDDSIFTGKFDDPQN
                     KPAVQPTPAEPAGPTPTPPKPLATPFDSDAT"
     gene            complement(3754..4608)
                     /locus_tag="VGP27_00320"
     CDS             complement(3754..4608)
                     /locus_tag="VGP27_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849793.1"
                     /translation="MDSEQLIRIALTVVTPILTAGIGIVALVIGDWRERRTQAGRRKI
                     SFEDASRQVEFAADWYKASREIAPDQEQQAAARAQVWLDEASDLVAESKPPPRSQKRS
                     VTARRLLLAYPMHRRGARVLRGFYYFFLGVVVLQVSGALGSAFGRTDTLGVPNYFSGG
                     LIYADLLGIVVYTLVAMAFRFWALRAEESLTADAPQRRMTVRDALLLCRLTGVGANIA
                     RIVYWFWLALIVLAVVAIAISASHDPRTLPSNVVALVAWAGWAVGLRYWAVSHSGRAA
                     GASPGAGC"
     gene            complement(4697..5839)
                     /locus_tag="VGP27_00325"
     CDS             complement(4697..5839)
                     /locus_tag="VGP27_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEV7849794.1"
                     /translation="MSTHPTELEAAVRAGLTKVIDPELRRPITELGMVKSVDVDADGG
                     VHVGVYLTTAACPKKTEISERVAAAVTDVPGTGSVKVSLDVMSDEQRTELRKQLRGDA
                     REPVIPFAQPGSLTRVYAVASGKGGVGKSSVTVNLAAALATRGLSVGVLDADIYGHSI
                     PRMMGTTERPTQVESMILPPIAHDVRVISIAQFTQGNTPVVWRGPMLHRALQQFLADV
                     YWGDLDVLLLDLPPGTGDIAISVAQLIPGAEILVVTTPQLAAAEVAERAGAIALQTRQ
                     RIVGVVENMSGLVLPDGTTMQVFGEGGGRQVAERLTTAVGADVPLLGQVPLDPALVSA
                     GDSGVPLVLSAPDSAAGKELRRIADALSSRKRGLAGMSLGLDPAGR"
     gene            complement(5899..>6933)
                     /locus_tag="VGP27_00330"
     CDS             complement(5899..>6933)
                     /locus_tag="VGP27_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEV7849795.1"
                     /translation="GTLRIPMIALNAYQNAERMMAVSYPGCGISWNLLAGIGRIESMH
                     ANGGATDVSGSPVRPIYGPSLDGTLPGNEVIELSRNSNRVTYARAIGPMQFLPGTWSR
                     YASDGNGDGKADVQNVFDASLAAARYLCSGGLNLRDQSQVMTAILRYNNSMAYAQNVL
                     GWAAAYATGVVPVDLPPITGPIPPIADVHLDNYQGLGPGLPMSVLGLPADDPLALVPL
                     ADMGRTDVASQMQMPGQMLGPLPGPTESGARGVATPGPAQGVPPWMQQPPQQNCAVFC
                     LGNNPPAAVQPQLPPEALPPGVVAPQAGPPGLVPGTAAELAPPPPGEAPPTQAAIGPQ
                     GVGPAPGPAA"
BASE COUNT         1172 a         2391 c         2319 g         1051 t
ORIGIN      
        1 ccgtgtccgc gacggcccga tgcccgcctc gttgaacgcc tgcttggcga ggcgctgatg
       61 ctcgggttcg acgtcgatcg tcgtcagcac accgtcctcg cgcatgccgg agagcaacca
      121 cagcccgctg acaccggctc ccgtaccgac ctcgacgacc gttttcgcgc cgctcagttt
      181 ggccagcacg ctcagcagcg ccccgactgc cggcgtgacg gcgccggctc cggtctcgac
      241 ggcgcggtcg cgcgccgcgg cgacgatggc gtcctcggag atggactgtt cggcgtgatt
      301 gacgatcgac tcggcgcggc tgaacggcgg ctggctcgcc gggtcatcgg gcatgtccgc
      361 agcgtagagc ggtgcccgag gggccaccac cacgacacgc ccgtgccgca atgctgaaat
      421 ccggcggaaa ttgggtgatt tgccctggtt agaagtgggg tgattgggta acaagttcat
      481 ttctcaggga aagttcagtc agctcatatg ccagccacac caccatcgga aaaggtaact
      541 cgcatggaac acggcggacg ctggcgctct gggaataacc atgttgaccg tcatgttgtc
      601 gcggatgtcg cgttgccgac aagcggcgaa cgctgtgatc tggaggattc gacgagcacc
      661 accactacgg ccgcccatct ggcgccggtt tcgatgccgc atctcgagcg gttcaccgac
      721 ggggaatggg tcgagccttc cgaagaacca cagggcaccg cggttttcga cgccaccggt
      781 gaccactcca ccatgccgtc gtgggacgag ctggtgcgtc agcacgccga tcgggtgtac
      841 cggctggcct accggctctc cggcaaccag cacgacgccg aggacctgac ccaggagacc
      901 ttcatccgcg tcttccggtc cgtgcagaac tatcagcccg gcaccttcga gggctggttg
      961 caccgcatca ccaccaacct gttcctcgac atggtccgca ggcgcgggcg catccgcatg
     1021 gaggcgctgc ccgaggacta cgaccgggtg cccgccgacg agcccaaccc cgagcagatc
     1081 tatcacgact cgcggctcgg acccgatctg caggccgcgc tcgactcgct gccgccggaa
     1141 ttccgcgcgg cggtggtgct gtgcgacatc gagggcttgt cgtacgagga gatcggcgca
     1201 acgctcggcg tgaagctggg caccgtacgc agccgcatcc accgtggcag gcaggccttg
     1261 agggaccact tggcgagcca ttccgccgac acggccaggt cagcctgacc gttcccgggt
     1321 gtcggacgac gtgtcccgcg ctacattcaa gtgagtaccg tctgtcgcga tagtgatggg
     1381 ccgagaggag cggggtgatg gccgacccgg ggcatgtgtt tcgtcgcgcg ttctcctggc
     1441 tgcccaccca gttcgcctcg cagagcgatt caccggtagg accgcgccag ttcggctcca
     1501 cagagcacct gtccaccgag gcggtcgccg ccttcgccga cggcgaactg cggatgaccg
     1561 cccatctgcg cgcggcgcac catctgtccc tgtgtacgca gtgcgccgcc gaggtagagg
     1621 cccagcgcca ggcccgcgcc gcgctgcggg attcccgtcc cattgccatt cccacttcgc
     1681 tactgggcct actggcgcag attccgcagg acacagcgca ggtagcgccc gacgagaacg
     1741 agcagacgca gtttgctgac gacacgatcc gcggccggcg caagcgccgg taatgtgggt
     1801 ggcagtccaa gcatcgacgc gcgccggcga atagcggcga ccaaatagag agtgatacac
     1861 gggtgaccaa gcaggaccag accgacggta gccaccgggt cgagccgcgc cctgtctcgc
     1921 gtccaccggt cgacccgtcc acgaccaggg cgttcggtag gcccgacggc gtcaacggct
     1981 ccttcaacgg cgcggagacg aagcccgatc agggcgagtt caccccgacc gaccagccgc
     2041 ccgatcccgt ccttcacgag gccttcggac ggccgaacgg ccgcggggac tccctgcagc
     2101 gccatcccgc cgacatcggc gcgctggacg ccgagcgcga caggcagaag ccgcaggagc
     2161 tagacgatcc gtggcgcgat cccggcgcgg cggccgcact cgggacgccc gccgtcccga
     2221 cgcccgcagc ccatcccgcc gccacgaacg tcggcaagct gggcgttcgc gacgtcctct
     2281 tcggcggtcg ggtgtcctat gtcgcgctcg ccgtgctggc gatcgtcgcg ctggtgatcg
     2341 gattcgccgg cggttgggtt ggtcgcaaaa ccgccgaggt cgtcgaggcg ttcaccacgt
     2401 cgaaggtgac actgtccacc gacaccaact ccgaacagcc gcaggggcgc ttcgcgaaag
     2461 tagctgcctc cgtagcggat tcggttgtca ccatcgaagc ggtcggtgac aacgaaggtg
     2521 cacagggctc cggcgtggtc gtcgaccccc gcggctacat cgtcacgaac aaccacgtga
     2581 tctccgaggc cgccaccaac ccgagccagt acaagctgtc ggtggtgttc aacgacggca
     2641 aatcggtgcc tgccaatctc gttggccgcg acccgaaaac cgatctggcc gtgttgaagg
     2701 tcgacaacgt cgacaacctc acggtggcga agctgggcga ctccgacgag ctgcacgtcg
     2761 gtgacgaggt catcgccgcg ggcgccccgc tcggcctgcg cagcaccgtc acccagggca
     2821 tcatcagcgc gttgcaccgg cctgttccgc tgtccggcga cggctccgac accgacaccg
     2881 tgatcgacgg cgtgcagacc gacgcggcca tcaaccacgg caactccggt ggcccgctga
     2941 tcaacatgaa ctccgacatc atcggcatca acacggctgg aaagtcgttg tccgacagtg
     3001 ccagcgggct cggcttcgcg atcccggtca acgaggtgaa gaacgtcgtc gaggcgttga
     3061 tcaaggacgg caagatcgca cacccgacgc tggggctgac cgcgcgctcg gtgagcaacg
     3121 acctcgcttc cggcgcgcag gtggccaacg tcaaggcggg cggcccggcg gagaaggcgg
     3181 gcattctcga gaacgacgtc gtggtgaaga tcggcgatcg cacggtcgcc gacgcggacg
     3241 aattcgtggt cgcggtgcgg cagttgaaga tcggtcagga cgcccccgtc gaggtggttc
     3301 gcgacggccg tcatgtgacg ctcaccgtga atcccggttc cgacaacagc acctgatgtt
     3361 cgccaacgtc ggctggggcg agatgctggt cctcttgatc gccggactgg tgatcctggg
     3421 gccggagcgg ctgcccggtg ccatccgctg gacatctgat gcgatgcgcc aggcccgtga
     3481 ctacatcagc ggggccacca aacagctgcg cgacgacctc ggtccggagt tcgacgacct
     3541 tcgcgagccg ctgagcgaac tgcagaagct gcgcggcatg acaccacgcg ccgcgctgac
     3601 caaacacctt ctcgacgggg acgactcgat cttcaccggc aagttcgacg acccacagaa
     3661 caaacctgcc gttcagccca ccccggcgga gccggcgggg ccgacgccaa cgccgcccaa
     3721 gccgctcgcg acgccgttcg acagcgacgc cacctagcac ccggcgcctg ggctcgcccc
     3781 cgctgcgcgt ccgctgtgcg atacggccca ataacgaagt ccaaccgccc atccggccca
     3841 ggcgacgagc gccacgacgt tggagggcag tgtccgtggg tcatgcgatg cgctgatcgc
     3901 gatcgccacg acggccagca cgatcaacgc cagccagaac cagtagacga tccgcgcgat
     3961 gttggcgcca acgcccgtga gccggcacaa gagcagcgca tcacgcacgg tcatccggcg
     4021 ttgcggcgca tccgcggtca gcgactcctc ggcgcgcagc gcccagaagc gaaacgccat
     4081 cgcgacgagt gtgtacacca cgatgccgag gagatcggcg tagatgaggc cgccggagaa
     4141 gtagttcggg acacccaggg tgtcggttct gccgaacgcc gagcccagcg ctccggagac
     4201 ctgcagaacc accacgccga ggaagaagta gtagaagccg cgaaggaccc gcgcgccgcg
     4261 acggtgcatc gggtaggcga ggagcaaccg ccgagcggtc acggaccgct tctgcgagcg
     4321 cggcggtggc ttggattcgg cgacgaggtc agacgcttcg tcgagccaaa cctgcgcccg
     4381 cgccgcggcc tgctgctcct ggtccggtgc gatttccctg ctggccttgt accagtcagc
     4441 cgcgaattcg acttgcctgc tggcatcttc gaacgagatc ttgcgccggc cggcctgcgt
     4501 tctgcgttcg cgccagtcac cgatcaccag cgcaacgatc ccgatcccgg ccgtcagaat
     4561 cggcgtgaca accgtcaacg caatgcggat gagttgttcg gagtccatcg cgcccagtct
     4621 tgccgcgaaa cggcattccg gcagggaaac gtcgagtaga cgctgcggga attccgtccc
     4681 gcgacgggcg ttagcgctag cggccggcgg gatccagacc cagcgacatc ccggcgagcc
     4741 cgcgcttgcg gctggacagc gcatcggcga tgcggcgtaa ctccttgcct gcggccgaat
     4801 ccggcgcgct gagcaccagc ggcaccccgg agtcgcccgc ggagacgagc gcgggatcca
     4861 gcggcacctg ccccagcagc ggcacgtccg cgccgacggc cgtcgtcagc cgctccgcga
     4921 cctgacggcc accgccctcg ccgaacacct gcatcgtcgt accgtccggc agcaccaggc
     4981 ccgacatgtt ctcgacgaca ccgacgatgc gttgacgcgt ctgcaacgcg atggcgcccg
     5041 cgcgctcggc gacctccgcc gcggccagtt gcggcgtcgt gacgacgagt atctcggcgc
     5101 cggggatcaa ctgcgccacc gagattgcga tgtcgccggt gcccggtggt agatcgagca
     5161 gcagcacatc cagatcgccc cagtacacat cggccaggaa ctgctgaagc gcccgatgca
     5221 gcatcggtcc ccgccacacc acgggcgtat tgccctgggt gaactgggcg atggagatga
     5281 cccggacatc gtgggcgatc ggcggcagga tcatcgactc gacctgcgtg ggccgttcag
     5341 tcgtgcccat catccgcgga atggagtgcc cgtagatgtc ggcgtcgaga acaccgaccg
     5401 acagtccgcg ggtcgccaac gcagcggcga ggttgaccgt cacgctggac ttgccgacac
     5461 cgcccttgcc cgaggcgacg gcgtacaccc gcgtcagcga gccgggctgg gcaaacggga
     5521 tgacgggttc tcgggcgtcg ccgcgcagtt gcttgcgcag ttcggtgcgc tgctcgtcgc
     5581 tcatcacgtc gaggctgacc ttgactgacc cggtgccggg cacgtcggtg acggcggcgg
     5641 cgacccgctc ggagatctcc gttttcttcg ggcacgccgc ggtggtcaga tacaccccga
     5701 cgtgcacacc gccgtcggcg tccacgtcga cgctcttgac catgccgagt tcggtgatcg
     5761 gcctgcgcaa ctcggggtcg atcaccttgg tcagcccggc gcggaccgcg gcttccagct
     5821 cagtgggatg tgttgacatc accgacgagt gtaggcgggt gcggtgaggc cgattcgcgc
     5881 gcggcctggg atcaggggtc aggctgccgg accgggcgcc ggtccgacgc cctgcggtcc
     5941 gatggcggct tgtgtcggtg gcgcctctcc cggcgggggt ggcgcgagtt cggcagccgt
     6001 gcccggcacg agcccgggag gcccggcctg cggcgcgacg acgcccgggg gcagtgcctc
     6061 gggcggcaat tgcggctgca ccgcagcagg cggattgttg ccgaggcaga acacggcgca
     6121 gttctgttgc ggcggctgtt gcatccacgg cggcacaccc tgggccgggc cgggtgtggc
     6181 gacccctcgt gcgcccgatt ccgtgggtcc cggcagcggc ccgagcatct gcccgggcat
     6241 ctgcatctgg ctggccacgt ccgtgcggcc catgtcggcc aacggaacca acgcgagcgg
     6301 atcgtcggcg ggcagcccga gcacactcat cggcaagccg ggtccgaggc cctggtagtt
     6361 gtccaggtgc acgtcggcga tcggcggaat aggacccgtg atcggcggga ggtccaccgg
     6421 cacgactccg gttgcgtaag ccgccgccca gcccagcacg ttctgggcgt acgccatcga
     6481 gttgttgtag cgcaggatcg cggtcatcac ctgcgactga tcgcgcaggt tcagcccgcc
     6541 gctgcacagg tagcgcgccg cggccaggct cgcgtcgaac acgttctgga cgtcggcctt
     6601 gccgtcgccg ttgccgtcgg acgcgtagcg cgaccacgtg ccgggcagga actgcatcgg
     6661 acctatcgcg cgggcatagg tgacccggtt ggagttgcgg ctcaactcga tgacctcgtt
     6721 gcccggcagg gtgccgtcca gcgagggccc gtagatgggc cgcaccggag aaccggacac
     6781 gtcggtggcg ccgccgttgg cgtgcatcga ttcgatgcgc ccgatacctg cgagcaggtt
     6841 ccaactgatg ccgcagcccg ggtaggaaac ggccatcatc cgttccgcgt tctgatacgc
     6901 gttcagcgcg atcatcggaa tgcgaagtgt gcc
//
LOCUS       DASYLX010000013         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_988591,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000013 DASYLX010000000
VERSION     DASYLX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988591"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGP27_00335"
     CDS             <1..365
                     /locus_tag="VGP27_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7849796.1"
                     /translation="PDSVAEIGDRVTRMIAEQPEIADYIGRALIDGRPVGDMIFDSLL
                     NSGMARWHRRSERGETRADLDLTWAAINSLVLALGALMMRSHIERHLPEPFTSPAQLE
                     RWQGSVNSLLREGLFKQQ"
     gene            complement(374..1216)
                     /locus_tag="VGP27_00340"
     CDS             complement(374..1216)
                     /locus_tag="VGP27_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7849797.1"
                     /translation="MTVSALSDRGLAESANNRGRRVIELRRGGQAPGGSYLYEGEGLI
                     TGWHSHEVHQIEYAIGGVVEVETASAHYLLPPQQAAWIPAGLEHQATMAPGVRTVAVM
                     FDPQMIPSGGDRARILAVSPLIREMMIYGLRWPIDRPRGDDVSDAFFRTLADLVIEAL
                     DHEAPLSLPTSEHPIVAAAMAYTKEHLQSVSADQVSRAVAVSERTLRRLFHDELSLSW
                     RTYLLHARMLRAMALLASPGQSVQDTSTAVGFDSLSSFTRAFAQFSGETPSTYRKRVA
                     REAL"
     gene            1294..2556
                     /locus_tag="VGP27_00345"
     CDS             1294..2556
                     /locus_tag="VGP27_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7849798.1"
                     /translation="MKEADLILVSIDDHVVEPPDMFLNHVPAKYKEEAPIVVTDDKGV
                     DQWMYQGRPQGVSGLNAVVSWPAEEWGRDPAGFAEMRPGVYDVHERVRDMNRNGILAS
                     MCFPTFTGFSARHLNMHREDVTLVMVSAYNDWHIDEWAGSYPDRFIPIAVLPTWNPEA
                     MCDEIRRVAAKGCRAVTMPELPHLEGLPSYHDDEYWGPVFRTLSEQNVVMCLHIGTGF
                     GAISMAPNAPIDNLIILATQVSAMCAQDLLWGPAMRNYPDLKFAFSEGGIGWIPFYLD
                     RSDRHYTNQKWLRRDFGDKLPSDVFREHSLACYVTDKTSLKLRHEIGIDIIAWECDYP
                     HSDCFWPDAPEQVLAELTAAGADEADINKITWENSCRFFGWDPFARTPREQATVAALR
                     AKATDVDVSIRPRKEWARLYEQKQLAKA"
     gene            complement(2562..3308)
                     /gene="yaaA"
                     /locus_tag="VGP27_00350"
     CDS             complement(2562..3308)
                     /gene="yaaA"
                     /locus_tag="VGP27_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV7849799.1"
                     /translation="MIVLLPPSETKRAGGDGPPLRLDALSFPELRPMRAVLIDELVAL
                     AADPPACRRALGISASQDHEIDRNATLEQAPTVAAINRYTGVLYDALDIETLKGARLS
                     RAHARLAIGSALFGIVRADDPIPAYRFSASSKLPGQPTLASRWRPVLEPVLAEIASEE
                     LVVDLRSGSYAALSRLPGAVRVDVVTEHADGQRTVVSHFNKAHKGRLARALVSSRAEP
                     DDASKVAAVARKAGMRVERDGNELTVVLVA"
     gene            3355..>4068
                     /locus_tag="VGP27_00355"
     CDS             3355..>4068
                     /locus_tag="VGP27_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7849800.1"
                     /translation="MRIIDADGHVAEGASLAVEAMRRWPEFIVPRTDSPGLVIEGRNY
                     PESSGPGAGCPTEHGISAAEGINCRSAEGVLGDADRDHIDTMVLYPSLGLCTPTLEDP
                     DFAAGFARLYNEWIADYCSSSNGRLHGIAVTPIEHGDVAIDIMREAKDLGLVATHVPP
                     ALKTRNLDHPDLDPFYAAAVELVMPLGIHGAPGVHLPKIGVDRFTNYIQVHCISFPFD
                     QMTAMTALVSGGVFDRHPDL"
BASE COUNT          731 a         1347 c         1329 g          661 t
ORIGIN      
        1 cgccggactc ggtcgccgag atcggcgacc gggtgacccg gatgatcgcc gagcaacccg
       61 agatcgcgga ttacatcggc cgcgccttga tcgacggcag gccggtcgga gacatgatct
      121 tcgactccct cctgaactcc ggcatggcgc gctggcaccg acgaagtgaa cgaggggaaa
      181 cgcgcgccga cctcgatctg acgtgggcgg cgatcaactc actggtgctg gcccttggcg
      241 ccttgatgat gcgcagtcat atcgagcggc acctgcctga gccgtttact tcacctgccc
      301 aactcgagcg ttggcaggga tcggtgaact cgctgctgcg cgagggactg ttcaagcagc
      361 agtagtagcg acgtcaaagc gcctcgcggg caaccctttt gcgataggtg gacggcgtct
      421 ccccgctgaa ctgcgcgaaa gcgcgggtga acgagctgag gctgtcgaag ccgactgccg
      481 tcgacgtgtc ctgcactgac tgccccggcg aggccagcag tgccattgcc cgaagcatcc
      541 gggcgtgcag cagataggtg cgccacgaca ggctcagttc gtcgtgaaag aggcgtcgca
      601 gggtgcgctc cgacaccgcc accgcgcggc tcacctggtc ggcgctcacc gattgcaggt
      661 gttctttggt gtaggccatc gcggcggcga cgatcggatg ctcggacgtc ggaaggctca
      721 gcggcgcttc gtgatccagg gcctcgatga ccaggtcggc aagagtccgg aagaacgcgt
      781 cggagacgtc gtcaccgcgc gggcggtcga tcggccaccg cagcccatag atcatcatct
      841 cccgaatgag cggtgacacc gccaggattc gggccctgtc gcccccactc ggaatcatct
      901 gcgggtcgaa catcactgcc acggtgcgta cgccgggcgc catcgtcgcc tggtgctcga
      961 gcccggccgg aatccacgcc gcctgttgag gaggcaacag gtagtgagcc gaggcggttt
     1021 cgacctcgac cacaccgccg atcgcgtact cgatctggtg cacttcgtgg ctgtgccagc
     1081 cggtgatgag gccttcacct tcgtagaggt agctcccacc gggcgcttgg ccgccgcggc
     1141 gtagctcgat gacccgccgg ccacgattgt tggccgattc ggctaaacct ctgtccgaaa
     1201 gcgcggagac ggtcacgccg ctagacggta ctacttgggt atgcagaccg cgcacgcgag
     1261 gagcgtcatg aaaccgcgca cgcaaggagc gaaatgaaag aggccgatct gatcctggtg
     1321 agcatcgatg accacgtcgt ggagccaccg gacatgttcc tcaaccatgt gcccgccaag
     1381 tacaaggaag aggcgccgat cgtcgtcacc gacgacaagg gcgtcgacca gtggatgtat
     1441 cagggcaggc cgcagggcgt cagcgggctg aacgcggtgg tgtcgtggcc ggccgaggag
     1501 tggggccgcg accccgcggg cttcgccgag atgcgcccag gcgtctacga cgttcacgaa
     1561 cgcgtccgcg acatgaaccg caacggcatc ctcgcgtcga tgtgcttccc gacgttcacc
     1621 ggcttctcgg cccggcacct caacatgcac cgcgaggacg tgacgctggt gatggtgtcg
     1681 gcctacaacg actggcacat cgacgagtgg gcgggcagct accccgaccg cttcatcccg
     1741 atcgcggtgc tgccgacatg gaaccccgag gcgatgtgcg acgagatccg ccgcgtcgcc
     1801 gccaagggct gccgcgccgt cacgatgccg gaacttcctc acctggaagg gcttccgagc
     1861 taccacgacg acgagtactg gggaccggtg ttccgcacac tgtccgagca gaacgtggtg
     1921 atgtgcctgc acatcggcac gggcttcggc gcgatcagca tggcgccgaa cgcgccgatc
     1981 gacaacctga tcatcctggc gacgcaggtg tcggcgatgt gcgcgcagga cctgctgtgg
     2041 ggaccggcga tgcgcaacta cccggatctg aaattcgcct tctccgaggg cggcatcggc
     2101 tggatcccgt tctacctgga ccgcagcgac cggcactaca ccaatcagaa gtggctgcgc
     2161 cgcgacttcg gcgacaagct gcccagcgac gtgttccgcg aacactcgtt ggcctgctac
     2221 gtcaccgaca agacctcgct gaagctgcgc cacgagatcg gtatcgacat catcgcctgg
     2281 gagtgcgact acccgcactc cgactgcttc tggcccgacg cgcccgagca ggtgctcgcc
     2341 gaactgaccg cagcgggcgc cgacgaggct gacatcaaca agatcacctg ggagaactcc
     2401 tgtcgattct tcggctggga tccgttcgcc cgcacgcccc gcgagcaggc gacggtcgcc
     2461 gcgttacggg ccaaggccac cgacgtcgac gtctcgatcc ggccgcgcaa ggagtgggcg
     2521 cgactctacg agcagaaaca gctcgccaag gcgtgaaatc gttaggcgac cagaacgaca
     2581 gtcaactcgt tcccgtcgcg ctccacccgc atccccgcct tgcgtgcaac ggcggccacc
     2641 ttggaagcgt cgtcgggttc ggcgcggctg ctgacaagcg ctcgcgcgag cctgcccttg
     2701 tgtgccttgt tgaagtgact gaccaccgtg cgctgcccgt cggcgtgttc ggtgacgacg
     2761 tcgacgcgca ctgcgccggg cagccggctc agcgccgcat acgatcccga acgcagatcg
     2821 acgaccagct cctcagaggc gatctcggcc agcacgggct cgagcaccgg ccgccagcgt
     2881 gaggccagcg tcggttgtcc cggcagcttt gacgacgcgg aaaaccggta ggcgggtatc
     2941 ggatcgtcgg cccgcacgat accgaacagg gccgaaccga ttgcgagacg cgcatgtgcg
     3001 cgcgacagcc ttgcgccctt gagggtttcg atgtcaagag cgtcatacag gacgccggtg
     3061 tatcggttga tcgcggccac agtcggagcc tgctcgagcg tggcgtttcg atcgatctcg
     3121 tgatcctgtg atgcggagat gccgagcgcg cgtctgcacg cgggtggatc cgccgccagc
     3181 gccaccaact cgtcgatgag tacggcgcgc atcggccgca gctcagggaa gctcaaagca
     3241 tccaggcgga gcggcggccc gtcacctccg gcccgcttcg tctccgacgg cggcagcagc
     3301 acgatcacga tcgaaaaggg tatatgtaat cgtgctggtc gtatctttac aaagatgcgg
     3361 atcatcgacg ccgacggaca cgtcgccgag ggggcatcgc tggccgtcga ggcgatgcgg
     3421 cgctggccgg aattcatcgt gccgcgtacg gactcgccag gcctggtgat cgagggccgc
     3481 aactatcccg aatccagcgg gccgggcgcc ggctgcccga ccgagcacgg catctccgcc
     3541 gcggagggca tcaactgcag atcggccgaa ggcgtactcg gcgacgccga tcgcgatcac
     3601 atcgacacga tggtgctcta tccaagcctt ggcctctgca cgccgacgct ggaggacccc
     3661 gacttcgcgg cgggcttcgc gcggctctac aacgagtgga tcgcggacta ctgcagttcg
     3721 tccaacggcc ggctgcacgg gatcgccgtc acgccgatcg aacacggcga cgtcgcgatc
     3781 gacatcatgc gtgaggcgaa ggatcttggc ctggtggcga cccacgtgcc gcccgcgctt
     3841 aagacccgca acctcgacca tcccgacctg gacccgtttt acgcggccgc cgtcgagttg
     3901 gtgatgccgc tgggcatcca tggcgcaccg ggtgtccacc tcccgaagat cggtgtcgac
     3961 cggttcacga actacattca ggtgcactgc atcagtttcc cgttcgacca gatgacggcg
     4021 atgacggcac tggtctcggg cggtgtgttc gaccggcacc ccgacttg
//
LOCUS       DASYLX010000014        17939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2963317,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000014 DASYLX010000000
VERSION     DASYLX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17939
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963317"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VGP27_00360"
     CDS             complement(<1..790)
                     /locus_tag="VGP27_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7849801.1"
                     /translation="MMIKSAIGAMLGASLILTGCADSGRTSDGHGYPSGPVTMTAGAN
                     PGSGFDLTIRSVVEALQKEKLVDVPLPVQNRPGASGADFLATMVERYKGVDDQVSVTS
                     LSMMMNQLRGKSKYGYDDVTMIARLMTEYFVVVTGPSSPFKNLGEIMTAIKSDPARVA
                     VGAAHDDEAPFDLLVSAAGGDPSSVRYVTHEGGGDQSAALLKGDIGVAIGGVSEFITL
                     VKSGGVAALGVLAEKRLPGLDAPTAREQGLDVTLSNWRGLYGPPD"
     gene            complement(886..1791)
                     /locus_tag="VGP27_00365"
     CDS             complement(886..1791)
                     /locus_tag="VGP27_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849802.1"
                     /translation="MIASIKKAARLAGMRPIPRWPARTEVNLRSKRILLTGASSGIGA
                     VAAQKLAAEGATVIAVARRETLLTEVVERIVACGGSATAIPVDLADLDQIDELVDLVG
                     PIDILINNAARSIRRPLAESLKRWHDIERVMQLNYYAPLRLTRGLAPGMIERGDGHII
                     NIATWRVLPESSPMFAAYNASKAALSAVGRVIDTEWGHLGVHSTTIYYPLVTTPMIAP
                     TRAYDGLPALTAEEAADWMVTAAKTRPIRIAPRKALALRALDVVAPRVLNNVLGRETN
                     RMNAKFEKPEVTNPSVEATMPVALP"
     gene            2118..2846
                     /locus_tag="VGP27_00370"
     CDS             2118..2846
                     /locus_tag="VGP27_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV7849803.1"
                     /translation="MPNMRRLRGRRIAVLAADGFEKVELTIPMRALQMTGAHVDVISL
                     RHGRIRGVNLHMPATRVGVDKTIGEADPADYDGLLLPGGFINPDLLRQSAEARQFVRE
                     FANNDKPIVTLCHGPWVLASAGLLDGRTLTSWPGIRDDLVNSGATWLDREVVKDGNLV
                     TSRGPQDMTSFVPAMIDHFADTAPEPRAAVPGQVSDPQMSSPVSWAVAALRWSPKPSI
                     PGALAGLMAGARLSPKSPLRKVLS"
     gene            complement(2897..5548)
                     /locus_tag="VGP27_00375"
     CDS             complement(2897..5548)
                     /locus_tag="VGP27_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7849804.1"
                     /translation="MAKRPGKGMGRALAQLLLERESMLAEMARLARQASRRTGTMLLL
                     HGEAGVGKSALVRGFLDSVATGTQVLLARCDPLTAPRRLGPLIDMLPQLPGAQAAGLR
                     AAIDATDSESIYTRLLELFGDGNPCVCVIEDAHWADGATLDLLRFVARRIESLPVLVI
                     VSYRDDDLGHEHPFTVVLGDMVNHASLTRIGLSPLSVDAVRMLAEGSGVNAEQLHRIT
                     GGNPFLLTEVLACSTDGSKVGTLPRGVAEAVRGRLARLSVPGREAAEATAVCGPLADP
                     QFLEKLRPGVAAGLGECVRAGVLVADGEYVRFRHELARQATLEQIPYYDRRVMHRLAL
                     DALAEPPIDPDALASLVLHAEQAGDDIAVVRYGPAAAKRAAAVGAHSDAAELYALSLR
                     HAGSTPDRYKVRWLERHAFSCYLSGNAGAAAASWREAITLRRKLGDHLAEGDDLRWLS
                     NMLSTLGDTTAAAEAAHMSLRLLEPLGATPQLAWSLVNLAQLGAYRYRDATATEYAAR
                     AGDVADHLGETSVRLPARMHAAVRTVRATDSGWDQLETEWREAMSSRGMAELGGLSSA
                     IICWTAAAHADVDRAERYIGESIGFCRAKRLDIFEVLVAGAGARIDVYRGEWARAIAT
                     AEDILTRPNLSPLHRIWPLISLALVRARRNQKPVMPLLDEALAAGEADDLFRIGAVWA
                     ARAEVAWLAGDDQTARAEAEKGLEVSRAHHDRWLGGQLLRWAHLAGSPATPTAPGAAA
                     PYDLEITGDWKAASDNWIRRGCPYDAAIAELGGDIPAVESALRTFRQLGARAAVDRAR
                     ERLVALRGASVRPHRADHPYGLTQREREILELLALGHSDAGIANTLFISQRTVNNHVH
                     AILGKLGVHNRTQAATYAFQQSDGRQR"
     gene            complement(6090..6986)
                     /locus_tag="VGP27_00380"
     CDS             complement(6090..6986)
                     /locus_tag="VGP27_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849805.1"
                     /translation="MKLAADGRPVDSNNTAIAIGGGAQAVATRGNGNYASAVGELSRA
                     NAGSGSGNKAIARGVQAEADAHGGNNNTAIAIGNPVPVDDGGNTANLASSRPTLKPTV
                     ARAGAITASPNEQASAASLQVKTSDNNSAIAVGDGATAGAGNGSNNYASAFGNRSTAN
                     AGNGDYNIASVRGNRSTAEAGGVIVRATEDNEFAAGGVRLVAGNNNTAIVRGNDSTAI
                     AGADRLRRATAARTAAEPSDPTTNKNTAIVHGNNSRAVATGNGSRATVTANNSSAQAR
                     DGQRVTVRRPNMNPPGNAEAAK"
     gene            complement(7975..8880)
                     /locus_tag="VGP27_00385"
     CDS             complement(7975..8880)
                     /locus_tag="VGP27_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849806.1"
                     /translation="MTFPWRPMRASWAIVAVSCLIGAFCCWAAPSAQAFYVQNHASIT
                     RSALPPDQVNEVAVLQILNGPPPGGGAMGSDSFATDQWRHIDNAKNPADICARAQQAW
                     SILSPVILNGSRPVGPGATVLANGPAARAAFGGLAHAQQDFYAHSNWVEDNIAAGQPD
                     RLAPSIFPTCNPGDFPADLHTGYFNILFSKQFPLDGCPPAGPPAGFPECHSALNKDGP
                     DTARGSQPAPGTGMTMYDVAAGLATTATTNLYGQIRDLVASTVTAQNPGVDGGCVAAK
                     LFQPDLLGPCAAFSNPLALPGAPRR"
     gene            complement(9065..9388)
                     /locus_tag="VGP27_00390"
     CDS             complement(9065..9388)
                     /locus_tag="VGP27_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849807.1"
                     /translation="MGACGNDTDYRGRHPEYIQEGTHLPLKGLIMRKTFTYIAPLVGA
                     AAAAAAIVAAPIAAAAPGPATAPSQNWTPSGSGSVCQSPGNVQVNDAPSPVEFYPYGG
                     EAFQR"
     gene            9758..10876
                     /locus_tag="VGP27_00395"
     CDS             9758..10876
                     /locus_tag="VGP27_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEV7849808.1"
                     /translation="MTADAALGNALQWGKRDDMIVRTEAPFNAEPVPSVLATEEITSV
                     DAFYARNHGLFPDIAAAQWQLTVDGLVETPLTLTFDQLSTAFDQHTVVATLACAGNRR
                     AELLRVRPIPGKDPWEHGAISTAQWCGVRLADVLTAAGVHRHDGLHVEFLAPDVAQEA
                     RPVQSYGSSIPLEKAMSEEVLLAWQMNGQPLPRAHGGPARVVVPGYIGARSVKWVTSV
                     VVRNSPSQNYFQALDYRILPPETDPDTVAEGEGISLSTLPLNCDILTPTDDDHIATGA
                     LTIRGWAMAGDGQGIGRVDVSLDGGHTWRQAELHPARSKWAWSPWSLTVDVAPGPLNI
                     TARAWDDKGMTQPESPASLWNPRGYGNNAYARVQLHVT"
     gene            11070..11531
                     /locus_tag="VGP27_00400"
     CDS             11070..11531
                     /locus_tag="VGP27_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849809.1"
                     /translation="MSIKLGYTTPLVGAVAAAVTITAAPLANATTTITTNPDSCAGTV
                     CGSPGNVQVNDAPPAAAFYRPTKMKKISVPGLLAALSVAAVVAAPVAAAAPASDQSAQ
                     DTTTSAASGSGVPQPCLGLGGTQSLCQSPGNVQINDAPPQVDYYPYAGGAT"
     gene            complement(11771..12361)
                     /locus_tag="VGP27_00405"
     CDS             complement(11771..12361)
                     /locus_tag="VGP27_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7849810.1"
                     /translation="MATKAAERPSARERLLVAADELFYRDGVHSTGIDAVIEKAGVAK
                     GSLYYIFGGKDELVAAYLRRRHEAFRERVEEAQAGIDDPGDKILALFDAVADYVSLPE
                     YRGCPFDSAAAEAPIGQCQVLAIQEYRDWLNQSALRLASDTGVPDSQALAEALIVLFN
                     GALASANTGPPARAAAMTAKRLARLTLAAAKASSSV"
     gene            12532..12939
                     /locus_tag="VGP27_00410"
     CDS             12532..12939
                     /locus_tag="VGP27_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7849811.1"
                     /translation="MDTPAEDRNRAFVLEAFDTLFNRRDYAGAQRYWSPDYIQHSAHI
                     APGREGLFELVKASPPDMRYENGLVVAHGEYAMLHGRFTNIGRPANWIAADIVRFENG
                     LLAEHWDVIQDEATRADSVSGLPMFGTAFPARA"
     gene            13070..13819
                     /locus_tag="VGP27_00415"
     CDS             13070..13819
                     /locus_tag="VGP27_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849812.1"
                     /translation="MARLDGKIAVITGGSSGIGLASAQRFVDEGAHVFITGRRQSELD
                     KAKALIGHSVTAVAVDSTKSADLDTLFVTVLEEKGALDILLVNSGRVEPEELGKVSEE
                     NFDLTFDLNARAALFTVQKALPLMREGGSVILVGSVAGYVGINGYSTYSATKAALRSY
                     VRTWTRESNDRGIRFNTLSPGPIDTPIMDGQAGSPEEADQIRATFAAAIPMNRMGRPE
                     EIAAAALFLASDDSSFVAGAELSVDGGFAQV"
     gene            13840..14850
                     /locus_tag="VGP27_00420"
     CDS             13840..14850
                     /locus_tag="VGP27_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7849813.1"
                     /translation="MKISAQFATSLHSAEHIAVAEQLGYDRAWLFDTPHESPDVWMML
                     ALAAERTTTIGLGPGVLVPSLRHPMVNASAAAALQALAPGRVAVAFGTGFAGARALGA
                     PPTTWSYLRDYVAAFRALLTGATAVWQGNRMRMMHPDGHAPARPIDVPILISALGPKG
                     LAVATELADGLFTVNGQTRYAARFDWAALGVHGTILADDESLESPRVRAAAGPGNALA
                     YHAAYEFGGDVTALPAGNVWLDTINATAPQDRHLAVHDQHLIALNRADTAAWAAGSWK
                     AITATTLTGTAEDMGRRVKELAAQHITEIIYQPTGPDIAAELEAFHAAAGTAVDPVTQ
                     DN"
     gene            14890..15627
                     /locus_tag="VGP27_00425"
     CDS             14890..15627
                     /locus_tag="VGP27_00425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849814.1"
                     /translation="MASSTIGRDLEGKAALVTGATSGIGRAAAVQLAAQGATVIVHGR
                     DVARGAAVVTEIENSGGSARFVGADLGEPAEALRLAAEAGEVDILVNNAGFAWFGPTE
                     KLAADTLIQLFAANVHAPYLPVSVLAPKMVAHGHGVIINVASRAGIVGQPTTAAYGAT
                     KAALSSLARSWATEYGPAGVRVNAISPGPVYTNAADRELFDAVGETTVLGRAGDPQEV
                     ADLIGFLASPRASYITGTNVPIDGGRR"
     gene            complement(15670..16047)
                     /locus_tag="VGP27_00430"
     CDS             complement(15670..16047)
                     /locus_tag="VGP27_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7849815.1"
                     /translation="MSTGGLVPALKSLARRSPIPIALEVAVPRRLPEVVEVAAYYVVA
                     EALTNAAKYAQASELAVTADADEEVLQLTVRDDGVGGATAGKGSGLIGLIDRVEALGG
                     QLVISSPVGGGTSLRVNLPLRYE"
     gene            16603..16812
                     /locus_tag="VGP27_00435"
     CDS             16603..16812
                     /locus_tag="VGP27_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849816.1"
                     /translation="MKPGYVATLAVAATALTIGLAPVASADVNVQQTPGNAQISASPG
                     AASQEAAQAQMPFGGDSNALLFHHR"
     gene            17271..>17939
                     /locus_tag="VGP27_00440"
     CDS             17271..>17939
                     /locus_tag="VGP27_00440"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005238953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV7849817.1"
                     /translation="MAFEENTDPLTTAAGAPVVDNQNSMSAGPRGPLLLQDLWLIEKL
                     AHFAREVIPERRMHAKGSGAFGTLRITHDISRYTKAAIFSQVGNSCEMFARFSTVAGE
                     RGAADAERDIRGYALRFYTTEGNWDVVGNNTPVFFHRDPLKFPDLNRAVKRDPRTNMR
                     DPENNWGFWTNLPESLHQVTITMSDRGIPKSFRHMHGYGSHAYSMINAEGVRHWVKFH
                     FRTQQ"
BASE COUNT         3276 a         5816 c         5688 g         3159 t
ORIGIN      
        1 tgtccggggg gccgtagagg cctcgccagt tggagagggt gacgtcgagg ccctgctctc
       61 gggcggtcgg agcgtcaaga ccgggcaagc gtttctcggc cagcacgcca agcgccgcaa
      121 cgcctcccga tttcaccagg gtgatgaact cgctgacccc gccaatggcc acgccgatgt
      181 cgcccttgag aagcgcagcg ctctggtcac cgcctccttc atgggtgacg tatctgacag
      241 aggacgggtc tccgcccgcc gcggacacca gcaggtcgaa gggggcctcg tcgtcgtggg
      301 cggcaccgac cgcaacgcgc gcggggtcgg acttgattgc cgtcatgatt tcaccgaggt
      361 tcttgaacgg ggaactgggg ccggtgacga cgacgaagta ctccgtcatc aggcgggcga
      421 tcatggtgac gtcgtcgtac ccgtacttgg acttaccgcg aagctggttc atcatcatcg
      481 acagggacgt gaccgaaacc tgatcgtcta cgcccttgta ccgctcgacc atggtggcca
      541 ggaagtcggc gccgctggca cccggccgat tctgcaccgg cagcggcacg tcgacgagct
      601 tctccttctg caaggcttcg acgacggagc ggatcgtgag gtcgaatcca ctcccggggt
      661 tggctcccgc cgtcatcgtt acagggcccg acggatagcc gtgtccgtcg gaggttcgcc
      721 cggagtccgc acaacccgtg agtatgagcg aagcacccag catggcgccg atcgcggatt
      781 tgatcatcat gggctgtgcg ctcctggctc gtaataggtc ggcgacgttc ccgggcctgc
      841 gatccacggc gaccgggcgc gtctgcacgt cactcatgac atgcgtcagg gaagggcaac
      901 cggcatggtc gcttccaccg acgggttggt gacttcgggc ttttcgaact tggcattcat
      961 tcggttggtc tcgcgcccca agacgttgtt gagcactcgg ggcgcgacga catccagcgc
     1021 tcgtagcgca agtgccttgc ggggtgcgat gcggatcggt ctggtcttgg cggccgtcac
     1081 catccagtcg gcggcctcct cggcggtgag cgcaggcaag ccatcgtaag cacgggtggg
     1141 cgcgatcatc ggcgtcgtca ccaacgggta gtagatggtg gtggaatgca cgccgagatg
     1201 gccccactcg gtgtcgatga ctcggccgac ggcgctcagt gcggccttgg aggcgttgta
     1261 agcggcgaac attggcgacg actccggcag cacgcgccag gtggcgatgt tgatgatgtg
     1321 tccgtcgccg cgttcgatca ttccgggggc caggcccctg gtgagacgca gtggtgcgta
     1381 gtagttcagc tgcatgacgc gctcgatgtc gtgccagcgt ttcagcgact cggccagcgg
     1441 cctgcggatg gatctggcgg cgttgttgat cagaatgtct atcgggccaa caagatcgac
     1501 caactcgtcg atctgatcga ggtcggccag gtccaccggt atggcggttg cactgccgcc
     1561 gcaggcgacg atccgctcga cgacctccgt cagaagggtc tcgcgacgtg ccaccgcgat
     1621 gaccgtcgcg ccttcggcgg cgagtttctg tgcggcaacc gccccgattc ccgaggacgc
     1681 gccggtcagc aagatccgct tgctccgcag attcacctcc gtgcgagccg gccatcgcgg
     1741 tattggccgc attccggcca gtcgcgcagc ctttttgatg ctcgcgatca caaactgcac
     1801 ccctgctccg gtcggcacac gtaggcgttc cgctaataat ctatggtgct tcgtccacgc
     1861 ccggagcaaa tttgcgtatt cgtcagagag attgtgatga acagctgatg aggtgggtgt
     1921 cgagttcgtt ggcaaggcac cgcgcgttga cacccgacgg cgcctctctc gtggtgtcac
     1981 accttacgaa aaacctactg ggggctcccg aacatcgatt cggaaccatg tgtggacggg
     2041 tcgcttgagg gtatggccgt agtgggtgag cagccagcca gctcgcttga ggcgctacct
     2101 agcagcggga ggaacccatg cccaacatgc gacggctacg cggccgaaga atcgccgtcc
     2161 tggcggccga cggcttcgag aaggtcgagt tgacgatacc gatgcgcgcg ttgcaaatga
     2221 caggcgcgca tgtcgatgtc atttccttgc ggcacggccg gatacgagga gtgaacctgc
     2281 atatgcccgc cacgcgggtc ggtgtggaca agacgatcgg cgaggccgat ccggctgact
     2341 acgacggtct gctgttgccg ggcggattca tcaaccccga cctgctgcgg cagtccgcgg
     2401 aggcacggca gttcgtgcgc gaattcgcca acaatgacaa gccgattgtc acgctgtgcc
     2461 acggcccgtg ggtgctggcg tcggcgggac tgctcgacgg gcggactctc acgtcatggc
     2521 ccggcatccg cgacgatttg gtgaactccg gcgccacctg gctcgacaga gaggtcgtca
     2581 aggacggaaa ccttgtcacc agccgcgggc cccaggacat gacatccttc gtccccgcca
     2641 tgatcgacca cttcgcggac accgcgccgg agccacgggc cgcagtaccg ggccaggtgt
     2701 cggatccgca gatgagctcg cctgtcagtt gggccgtcgc cgccctgcga tggtccccca
     2761 aaccgtccat accgggcgca cttgcgggtc tgatggcagg tgcgcggctg tcgcccaaat
     2821 caccgctacg aaaagtcttg tcctgaatcg gcccgtgtct gtgaagtagc gcctcggcgg
     2881 acagcgcgcc cggcgttcag cgctgccgcc cgtccgactg ctggaacgca taggtcgcgg
     2941 cttgggtgcg attgtggacg cctagcttgc ccaggatggc gtggacgtgg ttgttgacgg
     3001 ttcgttggct gatgaagagg gtgttggcga taccggcatc gctatgacca agggcgagca
     3061 attcgaggat ttcgcgctct cgttgggtca agccgtaggg atggtctgcg cgatgcggcc
     3121 gcacgctcgc accccgcagt gccacaaggc gttcgcgtgc tctgtctacg gcggcgcgcg
     3181 cacccaattg ccggaaggta cgcagggcgg actccacggc ggggatgtca ccgccaagtt
     3241 ccgcgatcgc cgcgtcgtat ggacagccgc gacggatcca gttgtcgctg gcggccttcc
     3301 aatcaccagt gatttccagg tcatacggag cggcagcgcc tggggccgtc ggcgtcgcgg
     3361 gtgagccggc caggtgcgcc cagcggagta gctgtccgcc gagccaccgg tcatggtgtg
     3421 cgcgggagac ctccagacct ttttcggctt cggctcgggc ggtctggtcg tcgcccgcaa
     3481 gccaggcgac ttcggcacga gcggcccaca ccgccccgat gcggaagaga tcatctgcct
     3541 ctccagcggc cagagcctca tccagcaatg gcatgacggg cttttgattc cggcgtgccc
     3601 gaacgagtgc cagcgagatc agtggccaaa tccgatgcag tgggctgaga ttcggccggg
     3661 tgaggatgtc ctcggcggtc gcaattgcgc gagcccattc gccgcgatac acgtcgatgc
     3721 gcgctccggc gcctgcgacg agcacctcga agatgtcgag gcgcttcgct cgacaaaacc
     3781 cgatcgactc gccaatgtag cgctcggcgc ggtcaacatc ggcatgcgcg gccgcggtcc
     3841 agcagatgat cgcgctactc aggccaccga gttctgccat ccccctgcta ctcatcgcct
     3901 cgcgccactc tgtctcaagc tgatcccaac cactgtccgt agcacgcacg gttctgacgg
     3961 cggcatgcat gcgagccgga agtctgacgc ttgtctcgcc caggtgatcg gccacgtcgc
     4021 ccgctcgcgc cgcgtactcc gtggccgtcg catcgcggta gcggtatgcc cccaattggg
     4081 cgaggtttac caatgaccaa gccaactgcg gtgtggctcc gagcggttcg aggagccgaa
     4141 gcgacatatg cgcggcctca gcggccgccg tggtatcgcc caacgtagac agcatgttcg
     4201 acagccagcg cagatcgtcg ccttcggcga ggtgatcacc gagcttgcgg cgcaacgtga
     4261 ttgcctcacg ccaggaagcc gctgccgcac ccgcgttacc ggacaggtag cacgagaaag
     4321 cgtgccgttc gagccagcgc accttgtacc gatcgggtgt ggatccggcg tggcgcaacg
     4381 aaagcgcata gagttcggcg gcatctgagt gtgcacccac tgccgcggcc cgcttggccg
     4441 cggctgggcc gtaacgcacg accgcaatgt cgtcaccggc ctgctcagcg tgtaagacca
     4501 gagaagccaa cgcatccggg tcaatcggtg gttcggcaag tgcgtccagc gccagccgat
     4561 gcatgaccct gcgatcgtag taagggattt gttcaagcgt cgcctggcgg gccagctcgt
     4621 gtcggaaacg cacatattca ccgtcggcga ccagcacgcc ggcacgcacg cattcgccca
     4681 aaccagccgc tacgcctggg cgcagcttct ccaaaaactg cgggtcggcg agcggaccgc
     4741 acaccgccgt cgcttccgcg gcttcccggc caggcactga tagtcgcgcc aagcggcccc
     4801 ttacagcttc ggccacgccg cggggcaagg tgcccacctt cgacccatcc gtgctgcacg
     4861 cgaggacttc ggtcagcaag aaggggttac ccccggtgat ccggtgcagc tgctcggcat
     4921 tgacaccgct accctcggcg agcatacgca ccgcatcaac gctcaatggc gacaggccga
     4981 ttcgcgtgag cgaggcatga ttgaccatgt cccccagcac cacggtaaag ggatgctcgt
     5041 gaccgagatc gtcatcgcgg taggacacga tcacgagtac cggtagcgac tcgatccgcc
     5101 gcgcgacgaa tcgtaaaaga tccaaggtgg caccgtcggc ccaatgcgcg tcttcgatga
     5161 cgcagacgca tgggttgccg tcgccgaaca gttccagcag cctcgtgtag atcgattcgc
     5221 tgtccgtggc gtcgatcgcc gcgcgcaggc ccgcggcctg agcgccggga agctgcggga
     5281 gcatgtcgat cagcgggccg agccgccgcg gcgcagttag cggatcgcaa cgggccagta
     5341 gaacctgcgt cccggtcgcg acggagtcga ggaacccacg caccaaggca gacttgccaa
     5401 ctccagcttc accgtgcagc aacaacatcg tgccggtacg gcgcgatgcc tgccgggcca
     5461 gtcgggccat ctcggcaagc atggattcgc gctcgagcag aagctgcgcc aaggctcgac
     5521 ccatcccctt tccgggacgc ttcgccactc gcgaaggatc ggctacagca aacctaacca
     5581 gacatgtaga ggccgcggtg cgggaccaga gcgcaccgac gctgtaccca caactcggcg
     5641 cggcacaatc cataaacaca cgtctatcag catatttggc tgcggcggca tcgtcgctcg
     5701 gtaccgaaga ttctgcagca attttagctg gttcggctgc agcaaagcgt ctgcacattt
     5761 atgaggccgg gtgtgctcga tctaaggaga aattatggct tggtgggcaa ctttcgatgc
     5821 atagcggcgc ggtggcaaaa attgacgcag cggcaaacat agcccgaaca cttggctctg
     5881 aagggcccaa ttatgtcaga tccggatagg ccaccgcggc tcagggcaaa cggatcaact
     5941 agcggttgac ggtcgcctcg cgaggcctca ctgcccggtt cagcgaaggc aagtgcggca
     6001 ctgcagcaaa ccagcgatgc cctagacaca gatggaagac gggaacgtgc caattgcacg
     6061 tccccgtctt cgcagagcgg atctgcttcc tactttgccg cctccgcatt tcccggcgga
     6121 ttcatattgg ggcggcgcac ggtgacccgt tgcccgtcgc gcgcctgtgc actgctgtta
     6181 ttggcggtca cggttgctcg actgccgttg ccggttgcaa cggcccgact gttgttgccg
     6241 tgcacgatgg ccgtgttctt gttagtcgtc gggtcactgg gttccgcggc agtgcgggcg
     6301 gcggttgcgc gcctgagccg gtcggctcca gcgatggcag tgctgtcgtt gccgcgcacg
     6361 atcgccgtgt tgttgttgcc cgctacgagt ctgacgccgc cggctgcgaa ctcgttgtcc
     6421 tcggtggcgc gcacgatgac gccgcccgcc tcggcggtac tgcgattgcc gcgaacgcta
     6481 gcgatgttgt agtcgccatt gccggcgttg gcggtactgc gattgccgaa tgcactggcg
     6541 taattgttgc tgccgttgcc ggcacccgcc gtcgcgccat cgcccactgc gatagccgag
     6601 ttgttgtcgc tggtcttaac ttggagactt gcagcggatg cctgctcgtt tggagacgct
     6661 gtaattgctc ctgctcgtgc caccgttggc ttcagcgtcg gcctggaact tgcgaggttg
     6721 gcagtattgc cgccgtcgtc gacagggacc gggttgccga tcgcgatcgc ggtgttgttg
     6781 ttgccgccat gagcatccgc ttctgcctgg acaccgcggg cgatggcctt gttgccgctg
     6841 cccgacccgg cattcgcccg actgagctca ccgaccgcgc tggcgtaatt gccgttgccg
     6901 cgcgttgcga ctgcctgagc gccgccgccg atcgcgatag cggtgttgtt gctgtcgacc
     6961 gggcgaccat cggcggcgag cttcacacca gaagctcctg caattgcggt cgtcccgcca
     7021 gacgccgcct ccaagctctg ctccggcgag gtgccaccgc ctgagtcgat agcgtttcca
     7081 attgcgatcg cggtgttgtt cttgccgacg gcggtcgccg atgcgccgac gccccaggaa
     7141 acggcgaggt tccctccacc agtggcggtc gctacggcgc cctcgccaat tgccagcgcg
     7201 aagtcgccag gagcgctggt ggtgcacccc gagccgacgg tgaaccatcc ccccgcggat
     7261 acgcaagtgg cattggcgtc tccggccgaa ctgagcgcgc cgaccgccat cgcccccgac
     7321 gccagcgtgc ctataaacaa tccgccgcaa aggcgtttgc ggtcggcact actggtgctt
     7381 acggatctgt gtgtacccat gatcttcccc tttgtcgctt tagctgtgta gcagtggcca
     7441 agtcctgctg aagcttgaat tcctctcgaa gcgatttgcc gacgtcctgc gcagctatga
     7501 gcctgcgcgc aagttgccat cgattacatg agtacaaatg cttatttttc ggatacgtca
     7561 ataccgtctg agttgtcgct ttggcgcagt tgagactggg tattcggggt tctcgaggag
     7621 cgtgtttatg cctcatatcc tgaccccagc cgaccaaggt cgtgtcaaaa ccttaagcaa
     7681 cgataatttg ctggatctgc attgctgtga actagattgg catatggatg tatgcgcgaa
     7741 tctgagtttg ctgcagctca gacacaatga gcattgctaa tgacctagca gttcagtcat
     7801 cgaatcggta gttagtcatc ccacttcgac ccgctgacgt gttgacgcct gacagccttc
     7861 cctcttttgc caaaactgaa ggtctgggca caatgtcggc cgatccctca caaccgaggt
     7921 gagcggcacg tactacgagc acacgccgcc gactgcgacg cggcagtcat cgcgtcagcg
     7981 tcgtggagct ccgggcagcg caagcggatt ggaaaaagcg gcgcagggcc ctaacaagtc
     8041 gggctggaac agtttcgccg cgacgcaacc tccgtcgacg ccagggttct gtgcggtaac
     8101 cgtgctggcg acgaggtcgc gtatctgtcc gtagaggtta gtcgtcgccg tggtagccaa
     8161 gccggctgcg acgtcgtaca tcgtcatacc ggttcctggt gctggctgac tgccacgcgc
     8221 cgtgtcgggc ccgtccttgt tcaacgccga gtggcactct ggaaaaccgg ccggcggccc
     8281 cgccggggga caaccgtcga gcgggaattg cttggagaat aggatattga agtatccggt
     8341 gtggagatcg gcggggaagt cgccgggatt acacgtcggg aagatcgagg gagccagccg
     8401 gtccggctgc cccgcggcga tgttgtcctc gacccaattg gaatgtgcgt agaaatcttg
     8461 ctgcgcgtgt gcgaggccgc cgaatgccgc gcgggcggcg ggtccgttcg ccaatacggt
     8521 ggcacctggg ccgacgggcc gcgacccatt caggatgacc ggcgacagaa tgctccacgc
     8581 ctgctgcgca cgggcacata tgtcggctgg attcttggcg ttgtcgatgt gacgccactg
     8641 gtcggtcgcg aacgagtcac ttcccatcgc gccacccccc ggcggcggcc cgttgagtat
     8701 ctgaaggacc gcaacctcgt tgacctgatc gggcgggagc gcactgcggg tgatgcttgc
     8761 gtgattctgg acgtagaacg cctgcgcact cggtgcggcc cagcagcaga aggcgccgat
     8821 caggcaggac accgcgacta tggcccacga ggccctcatt ggtcgccatg gaaaggtcat
     8881 cggaactcct agccggtgcc gacacctcgg cacgctatac aactgctacg ggtccccaac
     8941 cgccggattc attcccccga acggatcaag ataaacgcct agacgcacat tgttatcgac
     9001 ctgtcgtcgg ccgccgtccg tttcccaaga gcctcgatgg gctcgcggat acgttcgcgc
     9061 tacgtcagcg ctggaatgcc tcgccgccgt aggggtagaa ctcaaccggt gagggcgcgt
     9121 cgttgacctg aacgttgccg ggcgactggc agaccgatcc ggaaccgctt ggcgtccagt
     9181 tctgcgacgg agcggtcgcc ggtccgggcg cggcggcggc gatcggtgcg gcgacgatgg
     9241 ctgcggcagc tgccgccgcg ccgactaacg gtgcgatgta ggtgaacgtc tttcgcatga
     9301 ttaacccctt cagcggcagg tgcgtgcctt cctggatgta ttcagggtgg cgcccccggt
     9361 aatcggtgtc gttgccgcaa gcgcccatcc gacatgcggt cactcggaag ctggctatag
     9421 gtgcttccgg tgatgctgcg gcgagtcctg atgctgcgcg gcgctccggg cgccccaggg
     9481 accggtgtgt gcgcggccga ggcgaggtgt agaccttgcg ggtgacgcgc agatcaccgc
     9541 cagaccgggg ccggcagcgc agaacgcccc tcagcggtca gccttcttcc cggtgtccga
     9601 tctgcccagc ggatgacatg gcgcggaacg gtcaccaagg gaagcgattt catgctcgcg
     9661 gtcggcacgg tactggtgcc aacaggtttc acacacccgc tagcggtaac gacttcggca
     9721 ctatcagcgc ttagcgtggg gcaacaggag gtcgatgatg actgcggacg ccgcactagg
     9781 taacgcattg cagtggggca agcgcgacga catgatcgtg cggacggagg cgcccttcaa
     9841 cgccgaaccg gttccatcgg tgttggccac cgaggagatc acctcggtgg acgcgttcta
     9901 tgcccgtaat cacggtctct tccctgacat cgcagcagcg cagtggcagc tgacggttga
     9961 cggattggtc gaaactccgc tgacgctgac cttcgaccaa ttgagtaccg ccttcgatca
    10021 gcacactgtg gtggcgacgc tggcctgcgc gggcaaccgc cgcgcggagc tgctgcgggt
    10081 gcgcccgatt ccgggaaaag acccatggga acacggggca atctcgaccg cacagtggtg
    10141 cggagtacgt ctggccgacg tgctcaccgc ggctggcgtg catcgccacg acgggctgca
    10201 tgtggagttc ttggctcccg acgtcgccca ggaggcccgc ccagtgcagt cctacggcag
    10261 ctcgatcccg ctggaaaagg cgatgtctga ggaggtgctg ctggcctggc agatgaatgg
    10321 ccagcccctg ccccgggcac acggcggccc ggcgcgggtg gtggttcccg gctatatcgg
    10381 ggcccgcagt gttaaatggg taaccagcgt ggtcgtgcga aacagtccct cgcagaacta
    10441 cttccaggcc ctcgattacc gcatcttgcc gcccgagact gaccccgaca ccgtcgcaga
    10501 aggcgaaggc atctcactgt ccacactgcc gctcaactgc gacatcctca cacccaccga
    10561 cgacgaccac attgcgaccg gtgccctcac gatccgcggc tgggcgatgg ccggtgacgg
    10621 tcagggcatc ggacgcgtcg acgtgtccct cgacggtggt cacacctggc gccaagccga
    10681 actgcacccc gcccgcagca aatgggcatg gtcgccgtgg tcgctcaccg tcgatgtagc
    10741 gccgggaccg ttgaatatca ccgcacgcgc ctgggacgac aagggaatga cccagcccga
    10801 atcacctgcg tccctgtgga atccccgcgg ctacggcaat aacgcctacg cccgcgttca
    10861 actgcatgtc acctgagaca atgtctgctc gtcgtggcgg tgccgcgggc ctgaaccgta
    10921 aggcgttgcc gcaaaacccc acatgcggac cgggatgagg attcccgttg gcggcacgag
    10981 ggatgggtgc cagctgtgac gacatacgtt gcttgagagg cgattctgga acgcagacag
    11041 attgaacgag gatgtcaggg agattcatca tgagcatcaa attgggttac acgacaccac
    11101 tggtgggtgc cgtggccgct gccgtgacga tcaccgccgc gccacttgcc aacgcgacca
    11161 ccaccatcac cacgaatccg gactcctgtg ccggcacagt gtgcgggtcg cccggcaacg
    11221 ttcaggtcaa tgacgcccct ccggccgctg ccttctatcg gcccactaaa atgaagaaga
    11281 tctctgtccc tgggctgttg gcggccctca gcgtcgctgc cgtggtcgca gcgccggtag
    11341 cggcggccgc gcccgcatcg gatcagtcgg cgcaggacac gaccacctca gccgcgtccg
    11401 gttccggcgt gccgcagccg tgcctgggcc tgggtggcac acagtcgctg tgtcagtcac
    11461 caggcaatgt acagatcaat gatgcgccac cgcaggtgga ctactacccg tacgcgggtg
    11521 gcgccaccta atcggctccg tcagcccttg tcggccgact ggtctgaagc ggttacagct
    11581 cagcaaaatt catcacgatc gcctctgggt ggatgcaatt ccagtttgct gcacgtgccg
    11641 cccgcttcca gagccgggca acgcgggtga cgcgctggaa gacccgaaga ccacctgcga
    11701 caagggctct cggcggggtc gtcggcagac ggagacccaa ttcgtgtcgt cgatcgcgtt
    11761 tacgtcggaa tcagactgag ctggaggctt tcgccgctgc gagggtgagc ctggcaaggc
    11821 gtttggccgt catcgccgcg gcgcgcgccg gtgggcctgt gttcgcgctc gccagcgccc
    11881 cgttgaaaag gacgatgagc gcctcagcca gcgcctggga gtcgggcacg ccagtgtctg
    11941 aggcgaggcg gagtgcggat tggttcaacc aatcgcggta ctcctggatg gccagcacct
    12001 ggcactgacc gatcggcgct tcggcagccg cgctgtcgaa gggacagccg cggtactccg
    12061 gaagcgatac gtaatcagcg accgcatcga agagcgccag gatcttgtcc ccgggatcgt
    12121 cgataccggc ctgcgcctcc tcgacccgct cccgaaacgc ctcgtggcgc cgacgaaggt
    12181 aggcggcgac tagctcgtcc ttgccgccga aaatgtagta gagggagcct ttggcgactc
    12241 ccgccttctc gatgacggcg tcgatcccgg ttgagtgcac gccgtcccgg tagaacaact
    12301 cgtccgccgc gaccagcagt cgctcgcgcg ccgacggtcg ttccgctgcc ttcgtggcca
    12361 tcgcaccagc atatcagaca gatttgtctg aaactgcaga tggcgatcct ctcaggagcc
    12421 tcgcgtcctg catgttcgca ccgccagtga ccgccggggc gccatcgaca cggacatcag
    12481 ccggaattat tgtttgtacc ggtctgttca aagatttgaa ggaggcaaat catggacaca
    12541 ccagcagagg atcggaaccg ggcgttcgtc ttggaggcgt tcgacacgtt gttcaatcgg
    12601 cgtgactacg ccggcgcgca acggtattgg tcgcccgact atatccagca cagcgcgcac
    12661 atcgcgccgg gacgtgaagg cctgttcgag ctcgtcaagg cctcgccccc tgacatgcgc
    12721 tacgagaacg ggctggtcgt cgcccacggc gagtatgcga tgctgcacgg ccggttcacc
    12781 aacatcggcc ggcccgccaa ctggatagcg gccgacatag tcaggttcga aaacggtctg
    12841 ctcgccgaac actgggatgt catccaagac gaggccaccc gagcggactc cgtcagcggg
    12901 ctacccatgt tcggtacggc cttcccggca cgggcatgag ctgtccccac ccgggacgcg
    12961 agcggattgt tctggcgccc aacagttccc gcacaacgac aacattgtcg cagcaaagga
    13021 cgcgcgtcga gaacgtccaa cacacaccaa cggattgagg atgaaactga tggcacgatt
    13081 agatggcaag atcgccgtga tcaccggcgg cagcagcggt atcggactgg cgagcgcaca
    13141 aagattcgtc gacgaaggcg cccatgtctt catcaccggg cgtcggcaaa gcgagttgga
    13201 caaggccaag gcgttgatcg gccatagcgt gaccgcggtc gccgtcgaca gcaccaagag
    13261 cgcagacctg gacacgctgt tcgtgacggt ccttgaagag aaaggcgcgc tggacatctt
    13321 gctggtgaat tccggacggg tcgagcccga ggagctgggc aaggtaagcg aggagaactt
    13381 cgacctgacc tttgacctca acgcccgcgc agcccttttc actgtgcaga aggcgctgcc
    13441 gttgatgcgc gaaggcgggt cggtcattct ggtcggttcg gtcgcgggct acgtcggcat
    13501 caacggttac agcacctaca gcgcaaccaa ggccgcactg cggtcctacg tccggacgtg
    13561 gacgcgagag tccaacgacc gcggcatccg gttcaacaca ttgagtccgg ggccgatcga
    13621 cactcccatc atggacggcc aagccggctc gcccgaagaa gccgaccaga tccgcgcgac
    13681 gttcgccgcg gcgataccaa tgaaccggat gggccgcccg gaagagatcg ccgcagccgc
    13741 actgttcttg gcctccgacg acagcagctt cgtagccggc gccgagctga gcgtcgacgg
    13801 cggcttcgcg caggtctaag caaccactga gagtaggcca tgaagatctc cgcccagttc
    13861 gccacctcgt tgcactcggc tgaacacatt gcggtcgccg agcaactcgg ctacgaccgg
    13921 gcgtggttgt tcgacacacc ccacgaaagc cccgatgtgt ggatgatgct ggctctggcg
    13981 gccgaacgca cgaccaccat cggtctgggc ccgggtgtgc tcgtgcccag cctgcgccac
    14041 ccgatggtca acgcctcagc cgccgcggcg ttgcaggcgc tggcgccggg ccgggtcgcg
    14101 gtcgcgttcg gcaccggttt cgcaggcgcc cgcgcgttgg gcgccccgcc gaccacgtgg
    14161 tcgtatctgc gggattatgt ggccgcgttt cgcgctctgc tcactggtgc gacggcggtc
    14221 tggcagggca accggatgcg gatgatgcac cccgacgggc atgcgcctgc gcggccgatc
    14281 gatgtgccga ttctgatctc ggcgctgggc cccaaggggc tggccgtcgc gaccgagctg
    14341 gccgatgggt tgttcaccgt caatggccag acccgctacg ccgcccgctt cgactgggcc
    14401 gcactcgggg tacacggcac catcctggcc gacgacgaat ccctggagtc accacgggtg
    14461 cgggctgccg ccgggccggg aaacgcactg gcctaccacg ccgcctacga attcggcggc
    14521 gacgtcaccg ccctgccggc aggcaacgtg tggcttgaca cgatcaacgc caccgcaccg
    14581 caggatcgcc acctcgccgt gcacgaccaa cacctcatcg cgctgaatcg cgccgacact
    14641 gcagcctggg cggccggcag ctggaaagcc atcaccgcga caaccctcac cggcaccgcc
    14701 gaggacatgg gccgccgagt caaagaactc gccgcccaac acatcaccga aatcatctac
    14761 cagcccaccg gacccgacat cgctgccgaa ctcgaggcct tccacgccgc agccggcacc
    14821 gctgtagacc ctgttacaca agacaattga tccatcagac acatcggtac acactccgga
    14881 ggaacagtca tggcaagctc caccatcggt cgggatctgg aaggcaaagc cgcgctggtc
    14941 accggcgcga cgtcgggaat cggccgggct gctgcggtgc agctggccgc acaaggcgcc
    15001 accgtgatcg tgcacggccg cgacgtcgca cgcggcgccg ctgtggtgac agaaatcgaa
    15061 aacagtggag gctcagcccg attcgtcggc gccgacctgg gcgaacccgc agaagcgcta
    15121 cggctggcgg cggaggccgg cgaagtggac atcctggtca acaacgctgg attcgcctgg
    15181 ttcggcccca ccgaaaagct ggccgccgac accctaattc agttgttcgc cgccaacgtg
    15241 cacgccccct acctgccggt gtcggtacta gcgccgaaga tggtggccca cgggcacggc
    15301 gtcattatca acgtggccag ccgggccggg atcgtcggtc agcccacgac tgcggcctac
    15361 ggcgccacca aagcggccct gtcctcccta gcccgttcgt gggccaccga gtacgggcca
    15421 gcaggcgtcc gcgtcaacgc catatcgccg gggcccgtct ataccaatgc agccgaccgc
    15481 gaactgttcg atgccgtcgg tgagaccacc gtgctggggc gggcaggtga cccgcaggag
    15541 gtagccgacc tcatcggctt cctggcctct ccgcgcgcgt cctacatcac cggcaccaac
    15601 gttcccatcg acggtggccg acgatgacta ccgcagtcgt tgctcgaaac gcgccgacgc
    15661 cctgcgcgtt cactcgtagc gcaaaggcaa gttgacgcgc aatgaggtgc cgccaccaac
    15721 gggactcgag attacgagct gaccgcccaa cgcttcgaca cggtcgatga gcccgatgag
    15781 tccggagcct ttgccggcgg tggcgccgcc cacgccgtcg tctcgaacgg tcagctgcag
    15841 cacttcctca tcggcgtccg ccgttacggc gagctcggag gcctgcgcat atttggcggc
    15901 gttggtcaac gcctcggcca ccacgtaata agcggcgacc tctaccacct ccggcaatcg
    15961 cctcggcact gccacctcaa gggcgatcgg tatcggcgag cgacgagcca acgacttcag
    16021 tgccggaacc aatccgcctg ttgacacgat cgccgggtgc atcccgtgtg agatctcccg
    16081 cagctcctca catagtccgg taaggcccgt tcctattagg cagagcaggt ctcgtagcgc
    16141 gccgagctcg ctgggggacg acgttgcgtc aggaacttcg gccctgtcac ctcgtcggtt
    16201 acctggccgc cgaagctata gccgcctcga gagcgccacc acttcgagtg cggccccgcg
    16261 gttcgtattg ctgctagctg caaaagccga tgcacgctcg cctccacgag ggtatgccgc
    16321 agcacttgcc cctgagtttc cagttaacgg cgggctcata tgggtgttag cggtggcgac
    16381 atccgtttgc caacgcgcga tcattggatc agtccaaacg ggcgaacgcc caaacaggag
    16441 agctacatcc tcggtttcca cgagtggaac cacgacgagg cgaacaccac gaacggccaa
    16501 gtggacgttc cgttctgaag caacgaggcc gacagtcggc cgcgtcgcga ccgcccatcc
    16561 tcggatgcct tggcggatcg cacctaacca gaggagaaga aaatgaaacc gggttatgtc
    16621 gcaacactgg ccgtcgccgc tactgcgctg acaattggac ttgctccggt tgcctcggct
    16681 gatgtcaacg ttcagcagac tccaggcaac gcgcagatca gcgctagccc cggagctgcg
    16741 tcgcaagagg ccgcccaggc acagatgccc ttcggcgggg attcgaacgc tctgctcttc
    16801 caccaccgct gacggcgacg gttcgccaag ggcgggaccg ttagagtcca gccctacgcg
    16861 cgattggcgc ttgtggcaca aagcaaaacg aatcattcga catggtgacg gcgaggctct
    16921 tcgaacgtca atcgggcagc acaccgcaat atagcaagtg gcgcatgcta tttgggcggc
    16981 gaccagaatt caagccagcc gctcgctcaa ttgggcgcca tcagcgagga caccgaaaca
    17041 cccggctggc ccgagtgaaa tgatttccgc aagaggatat ttgagatcaa cgacacggag
    17101 acttccggcg cgtgaaaaag ccgcatcggc ggccccaagg cgcaccgagt gtccgaatct
    17161 gtgggttact tgtcgattcc cgtgctgcag cgatgactca tgctcagcga aattggagtc
    17221 agtcccacca gaactgacat taagcctcac ccaccaccta ggagaagaaa atggcattcg
    17281 aggagaacac cgacccgttg acaaccgcgg cgggagcacc cgttgtggac aatcagaaca
    17341 gcatgagcgc cggcccacgc ggcccgctgc tgctgcagga cttgtggctt attgagaagc
    17401 tggcccactt cgcccgcgaa gtcatccccg agcggcgcat gcacgccaag ggttcgggtg
    17461 ccttcggcac gttgcgcatc acccacgaca tcagccggta caccaaggcg gcgatcttca
    17521 gccaggtcgg caacagctgc gagatgttcg cccggttctc cacagtggcc ggcgagcgtg
    17581 gagccgccga cgccgagcgc gacatccgcg gctacgcgtt gcgcttctac accaccgaag
    17641 gcaactggga cgtggtgggc aataacacgc cggtgttctt ccaccgcgac ccgctgaagt
    17701 tccccgacct caaccgcgcc gtcaagcgcg acccgcgcac caatatgcgt gacccggaga
    17761 acaactgggg cttttggacc aacctgcccg aatcgctgca ccaggtgacc atcaccatga
    17821 gcgaccgcgg catcccgaag tccttccggc acatgcacgg ctacggctca cacgcctaca
    17881 gcatgatcaa tgccgaaggt gtgcggcatt gggtgaagtt tcacttccgc acccagcag
//
LOCUS       DASYLX010000015         7257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1317821,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000015 DASYLX010000000
VERSION     DASYLX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317821"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGP27_00445"
     CDS             complement(<1..353)
                     /locus_tag="VGP27_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7849818.1"
                     /translation="MPASAQPVSLTGRDIQLSFGPSAVLRGVDIDVPAGTTAAVIGPS
                     GSGKSTLLRTLNRLYEPAKGDILLDGKSVLRDNPDALRQRIGMVFQQFNLFPHRSVLD
                     NVTLAPRKLKKLGAD"
     gene            complement(353..2152)
                     /locus_tag="VGP27_00450"
     CDS             complement(353..2152)
                     /locus_tag="VGP27_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV7849819.1"
                     /translation="MTPFTGRIGKAFVLVLVGLLVFSTPFAGPAAADVDQCAPPGVDS
                     ASAVPTNLASAAQGPGEDKYTTPTVQPLDTVKVDALSLGTPGVLTVGTLSDAPPSICI
                     NSAGQFTGFDNELLRAAAEKLGLRINFVGTEFSGLLAQVASRRFDVGSSSITTTDSRR
                     KTVGFTNGYDFGYFSLVVPTGSAITGFDKLAAGQRIGVVQGTVQEAYVVDSLHLDPVK
                     FPDYNTLYASLKTRQVDAWVAPSQQAQGTVQPGDPAEIVENTFSLDNFVAYAVANENK
                     PLIDALNSGLDAVIADGTWSRLYSDWVPRELPSGWKPGSKAAPVPKLPDFAAIAASHQ
                     GETADSTAAQPNSTMSQLADSFLDWDLYKQAVPTLLKTGLPNTLILTVSASVIGLLLG
                     MALAVAGISRARWLRWPARVYTDIFRGLPEVVIILLIGLGIGPVVGGLTGNNPYPLGI
                     AALGLMAAAYIGEIFRSGIQSVEAGQLEASRALGFSYSSSMRLVVVPQGIRRVLPALV
                     NQFISLLKASSLVYFLGLVANQRELFQVGRDLTAQTGNLSPLVAAGLFYLALTIPLTH
                     LVNYIDARLRSGKPPEPEDPLSPVNPASSLEMI"
     gene            complement(2202..2888)
                     /locus_tag="VGP27_00455"
     CDS             complement(2202..2888)
                     /locus_tag="VGP27_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7849820.1"
                     /translation="MPKRYGVKEKDQVVSHIINLVLTGKLRSGDRVDRNEVAQELGLS
                     RVPIQEAVVQLEHDGILSTRYHRGAFVERFDESVVLEHHELHGLLLGIASARAANDPR
                     PHVLAQLDSLTDFMRANRESRAFQEAAWQYRQTINDEYAGPRLLAEIRASQSFMPRSF
                     WVTYMNNHDEMLPFLEAETAAIHTHDPEGARAANAGRSALMGRIMVAELVRRGVFSPS
                     SLAKQPSVAF"
     gene            complement(2985..3779)
                     /locus_tag="VGP27_00460"
     CDS             complement(2985..3779)
                     /locus_tag="VGP27_00460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7849821.1"
                     /translation="MSPSIRVGVQLQPQHSPSYSHLRDAVRRCEDIGVDVAFNWDHFF
                     PLYGDPNGPHYECWTMLGAWAEQTSRIQIGALVTCNSYRNPELLADMARTVDHISDGR
                     LILGIGSGWKQKDYDEYGYDFGTAGSRLDDLAAAFPRITSRLGKLNPQPTRDIPVLIG
                     GGGEKKTLRLVAEYGDIWHSFSDSREYPQKSEVLARHCADVGRDPDAIERSAGVSGEE
                     TDALLAEADALADLGVTLLTVGANGPDYDLSAAEALCRWRDQQAGK"
     gene            complement(3802..5874)
                     /locus_tag="VGP27_00465"
     CDS             complement(3802..5874)
                     /locus_tag="VGP27_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEV7849822.1"
                     /translation="MALVPLNLFVTHDGKSKRQHVTCRFKCGDACSKPVRNTSDNEYF
                     GDIAKAVSRRSMLQAGGIAVLAVGAGSLLAACGAAEQPAATPTSSAGPPETPPGMKFK
                     AVAPNSEDAVVIPDGYKQGVVISWGDPIIDGAPKFDVRNQTGAAQRGQFGFNNDFAGL
                     LPIDGEPNRFLLVTNHEYVDPLFMFPGYNEDAPTRDQFDVEVAAIGMGVVEVERVGGG
                     GLKPVVGRYNRRITADTPMTLVGPAAGTDFVKTSADPAGRTVAGTFANCSGGVTPWGT
                     VLSGEENFQDYFGAAEGTAKPDPVGAERFDRYGIPLKPSALSWETFDPRFDVAKNPNE
                     ANRFGYIVELNPWDPNSTPVKHSAMGRFRHEAANIYITGDDTVVAYSGDDERFDYMYK
                     FVSSKKMQPGKDPSALAQNMTLLDEGTLYVAKLSSDIPAGQIDGSGKLPAKGFFSGTG
                     TWLPLLRSGPGGQAESLVPGVTAQEAAVFTRFAADKAGATKMDRPEDFEANPKTGKVY
                     VALTNNDERGAPGEAPPDAANPRNDNKSGQILEITDNHAGTDFTWELLLVCGDPEAAD
                     TYFAGFDKSKVSPISCPDNVAFDSHGNLWISTDGNALDSNDGLFAVALDGPNRGETKQ
                     FLTVPLGAETCGPVITDDLVTVCVQHPGEHDDNSVDNPFSRWPEGGNGTARPSVVAVW
                     KSDGQIGV"
     gene            complement(5987..6901)
                     /locus_tag="VGP27_00470"
     CDS             complement(5987..6901)
                     /locus_tag="VGP27_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7849823.1"
                     /translation="MAEIPDDELVNVDEFALLPENAEQIGHTGPLPSVARVESGDISA
                     LKWGDEPPEVVFLHGGGQNAHTWDTVILGLDVPALAIDLPGHGRSAWREDGDYGPRLN
                     ADTLRPVLRDWAPHPRLVVGMSLGGLTALRIAATEPALVPELLLVDVTPSAPERHEQM
                     TKAQMGTVALVQGDRTFPSFEAMLDVTVQASPHRDRKSLRRGVFHNSKHNDDGTWTWR
                     YDTFRSKNKEMPDSFTGLWEDVPAITMPTTLVRGANSFFVNDEDADAFAKGAPGFQRT
                     HIVADSGHSVQGDQPRALVDILRGVLDA"
     gene            6968..>7257
                     /locus_tag="VGP27_00475"
     CDS             6968..>7257
                     /locus_tag="VGP27_00475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7849824.1"
                     /translation="MVPKTHAPKGLTLRTAEDADWPAMALLAATCFGSWRPEESNEAW
                     RTMMPVDGGVVACDGPDVVGVALYLDLELTVPGGAVLPMAGVSWVAVSPTHRR"
BASE COUNT         1186 a         2275 c         2496 g         1300 t
ORIGIN      
        1 tcgtcggcgc cgagtttctt gagcttgcgc ggtgccagcg tcacgttgtc gagcacggag
       61 cggtgcggga acaggttgaa ctgctggaac accatgccga tccgttggcg cagagcgtcg
      121 gggttgtcgc gcagcaccga cttaccgtcc aagaggatgt cgcccttggc cggctcgtag
      181 agccgattga gcgtgcgcag cagtgtcgac ttacccgagc ccgacggccc gatcaccgcg
      241 gcggtcgtgc ctgcgggcac gtcgatgtca acgccgcgca acaccgcgct ggggccgaac
      301 gacaactgaa tgtccctgcc ggtcaacgag actggctgtg cactggccgg catcagatca
      361 tctccagcga cgatgccggg ttgaccggcg acagtggatc ctccggctcg ggaggtttgc
      421 cgctgcgcaa gcgtgcatcg atgtagttga ccaggtgggt cagcggaatg gtcagcgcca
      481 gatagaacag gcctgcggcc accagcggtg acaggttgcc ggtctgcgcg gtgagatcgc
      541 ggccgacctg gaagagctcg cgctggttgg cgacaaggcc gaggaaatac accagcgaac
      601 tggccttgag cagtgagatg aactggttca cgagcgcagg cagcacccgt cgaatccctt
      661 gtggcaccac cacaagacgc atcgacgacg agtagctgaa gcccaacgcc cgcgaagcct
      721 cgagctgacc ggcctcgacg ctttggatcc cggagcggaa tatttcaccg atgtaggccg
      781 ccgccatcag gccgagtgcc gcgatgccga gcggataggg attgtttccg gtcagcccgc
      841 cgaccaccgg gccgataccg aggccgatca gcaggatgat caccacttcg ggcagaccgc
      901 ggaagatgtc ggtgtacacc cgcgcgggcc agcgcagcca acgggcgcgg gaaatgcccg
      961 cgacggccag cgccattccg agtagcagcc cgatcacgct ggcgctgacg gtcaggatca
     1021 gggtgttcgg caggcctgtc ttcaacaggg tggggaccgc ctgcttgtac aggtcccaat
     1081 cgaggaatga gtccgcgagc tgcgacattg tcgaattcgg ttgcgccgca gttgaatccg
     1141 ccgtctcgcc ttggtggctg gcggcgatgg ccgcgaaatc aggcagtttc ggtacgggcg
     1201 cggccttgga ccccggtttc cacccggagg gcagctcgcg cggcacccag tcggagtaca
     1261 gccgcgacca ggtgccgtcg gcgatgacgg catccagccc cgagttgagc gcgtcgatca
     1321 gcggcttgtt ttcattggcg acggcgtagg cgacgaaatt gtcgaggctg aaggtgtttt
     1381 cgacgatctc ggccgggtcg ccgggctgca cggtgccctg cgcctgctgt gacggcgcca
     1441 cccacgcgtc gacctggcgg gtcttcaagc tggcgtagag ggtgttgtag tcggggaact
     1501 tgaccgggtc caggtgcagc gagtcgacga cgtaggcctc ctgcacggtg ccctgcacga
     1561 cgccgatacg ctgcccggcg gcgagtttgt cgaatccggt gatcgccgaa ccggtcggca
     1621 cgaccagcga gaagtagccg aagtcgtagc cgttggtgaa cccgacggtc ttgcgccggg
     1681 agtcggtggt ggtgatcgac gacgatccga cgtcgaagcg ccgcgatgcc acctgggcga
     1741 ggagtccgga gaactcggtg ccgacgaaat tgattcgcaa tccgagcttt tcggcggccg
     1801 cgcgtagtag ctcgttgtcg aagccggtga actggcccgc tgagttgatg caaatgctgg
     1861 gcggcgcgtc ggacagcgtg ccgaccgtga gcacgccggg cgtgccaaga ctgagcgcgt
     1921 cgaccttgac ggtgtcgagc ggctgcaccg tcggcgtcgt gtacttgtcc tcgccgggtc
     1981 cttgggcggc ggacgcgagg ttcgtcggaa ctgcgctggc gctgtcgacg ccaggcggcg
     2041 cgcactggtc gacgtcggca gccgcgggcc cggcgaaagg agtgctgaat accagcagtc
     2101 cgaccaggac caaaacgaac gctttaccta tccggcccgt gaatggggtc attggagtca
     2161 acgtagcggg acggaacggg ttggaaccgg cttcagctca ttcagaacgc aaccgatggc
     2221 tgcttggcca ggctcgaggg actgaacacg ccgcggcgca caagttcggc caccatgatc
     2281 cggcccatga gcgccgaacg gccggcattc gcggcgcgcg caccctccgg gtcatgggtg
     2341 tggatcgccg cggtctcggc ttcgaggaac ggcagcatct cgtcatggtt gttcatgtag
     2401 gtgacccaga acgagcgggg catgaagctt tgggaggcac ggatctcggc aagcagtcgt
     2461 ggtccggcgt attcgtcgtt gatggtctgc cggtactgcc aggccgcttc ctggaacgcg
     2521 cgcgactccc ggttggcccg catgaagtcg gtcagcgagt ccagctgggc gagcacgtgc
     2581 ggacgcgggt cattggccgc gcgcgcggat gcgatgccga ggaggaggcc gtggagctcg
     2641 tggtgctcga gtacgacgga ctcgtcgaag cgctcgacga acgcgccgcg gtgatagcgc
     2701 gtggacagga tgccgtcgtg ctccagctga acgacggcct cctgaatcgg cacacggctc
     2761 aggccgagct cttgggccac ctcattgcgg tcgacccgat caccggagcg cagcttgccg
     2821 gtgagcacca ggttgatgat gtgcgaaaca acctggtcct tttctttgac tccataccgc
     2881 ttcggcatag cggcgacagt taatcacggg aattggtcag tcggcggcga ttgaccgaat
     2941 aggactcttc tgtgtcgccg gattccgacg cagcccggcc tgaattattt cccggcttgt
     3001 tgatcgcgcc agcggcacaa cgcttctgcc gcgctcaggt cgtagtcggg accgttggcg
     3061 ccgacggtga gaagggtgac tccgaggtcg gcgagtgcgt ctgcttcagc gagcagcgcg
     3121 tcggtttctt cgccggagac accggccgag cgctcgatgg catcagggtc gcgaccgacg
     3181 tccgcgcagt gccgcgcgag cacctccgat ttctgcggat attctctgct gtcgctgaac
     3241 gagtgccaga tgtcgccgta ttcggcgacc agccgcaggg ttttcttctc gccgccgccg
     3301 ccgatcagca ccgggatgtc gcgggtcggc tgcgggttga gctttcccag tcgcgacgtg
     3361 atgcgcggga acgccgcggc cagatcgtcg aggcggctgc ccgcggtgcc gaagtcgtag
     3421 ccgtactcgt cgtagtcctt ctgcttccag cccgacccga tgccgaggat cagccggccg
     3481 tcggagatgt ggtcgacggt gcgcgccatg tcggccagca attccgggtt gcggtacgaa
     3541 ttgcaggtca ccagcgcgcc gatttgaatg cgcgaagtct gttcagccca cgcaccgagc
     3601 atcgtccagc attcgtaatg tggaccgttc gggtcgccgt agagcgggaa gaagtggtcc
     3661 cagttgaagg cgacgtcgac gccgatgtcc tcgcagcggc ggaccgcgtc acggagatgg
     3721 ctgtaggaag gtgaatgttg cggctgcagc tgcacgccga cacggatcga aggggacacc
     3781 ggtcaatcat tcccgattcg gctacacgcc aatctgacca tccgacttcc agacagcgac
     3841 gaccgacggt cgcgccgtgc cgttgccgcc ttccggccac cgcgagaacg gattgtcgac
     3901 gctgttgtcg tcgtgctcac cgggatgctg cacgcacacg gtcaccagat cgtcggtgat
     3961 gacgggcccg caggtttccg cgccgagcgg cacggtgagg aactgcttgg tctccccgcg
     4021 gttggggccg tccagcgcga cggcgaacaa tccgtcgttg gagtccaacg cgttgccgtc
     4081 ggtcgagatc cacaggttgc cgtggctgtc gaacgccacg ttgtcgggac acgaaatcgg
     4141 gctgaccttc gacttgtcga acccggcgaa gtaggtgtcg gctgcctccg ggtcgccgca
     4201 gaccagaagc agctcccacg tgaagtccgt accggcgtgg ttgtcggtga tctcgaggat
     4261 ctgcccgctc ttgttgtcgt tgcgcgggtt ggcggcatcc ggcggcgctt cgcccggtgc
     4321 cccgcgttca tcgttgttgg tcagcgcgac gtacaccttg ccggtcttgg ggttcgcctc
     4381 gaagtcctcg ggccggtcca tcttggtggc gccggctttg tcggcggcga atcgggtgaa
     4441 caccgccgcc tcttgcgccg tcacaccggg cacgagcgac tcggcctgac cgcccggacc
     4501 cgagcgcaac agcggaagcc atgttcccgt cccgctgaag aaccccttcg ccggcagctt
     4561 gcccgagccg tcgatctggc ccgccgggat gtcgctggac agcttcgcaa cgtacagggt
     4621 gccctcgtcg agcagcgtca tgttctgggc cagcgccgag ggatccttcc ccggctgcat
     4681 cttcttgctc gagacgaact tgtacatgta gtcgaagcgt tcgtcgtcgc cgctgtaggc
     4741 aacgacggtg tcgtcgccgg tgatgtagat gttggccgcc tcatggcgga accggcccat
     4801 cgcgctgtgt ttgaccgggg tggagttggg gtcccacggg ttgagctcga cgatgtagcc
     4861 gaaccggttg gcctcgttcg ggttcttggc gacgtcgaag cgtgggtcga aggtctccca
     4921 cgacagcgcc gacggcttca gcgggattcc gtagcggtcg aagcgctccg cgcccaccgg
     4981 atcgggcttc gccgtgccct cggcggctcc gaagtagtcc tgaaagttct cctcaccgga
     5041 aagtactgtg ccccaggggg ttacgccccc agagcagttc gcgaaggtgc cggcgacggt
     5101 gcggcctgcg gggtcggcgg acgtcttgac gaagtcggtg cccgcggcgg gccccaccag
     5161 tgtcatcggg gtatcggcgg tgatgcgccg gttgtaccgg cccaccacgg gtttgaggcc
     5221 gcccccgcca acccgctcca cctcgacgac gcccatgccg atcgccgcca cctcgacgtc
     5281 gaactgatca cgggtgggcg cgtcctcgtt gtagccgggg aacatgaaca gcggatcgac
     5341 gtactcgtga ttggtgacca acaggaaccg gttcggttcc ccgtcgatcg gcaacagccc
     5401 cgcgaagtcg ttgttgaagc cgaactgacc gcgctgcgcg gcgccagttt gattgcgcac
     5461 gtcgaacttc ggtgcgccgt cgatgatcgg gtcgccccag ctgatcacca cgccctgctt
     5521 gtagccgtcc ggaatgacca cggcgtcctc gctgttgggc gcgacggcct tgaacttcat
     5581 gcccggcggc gtctcggggg gcccggccga cgaggtcggg gtggcggcgg gttgttcggc
     5641 cgcaccacac gcggccaaca gcgagccggc gccgaccgcc aacaccgcga tgccgccggc
     5701 ttgcagcatc gagcgccgtg aaaccgcctt tgcgatgtct ccgaagtatt cgttgtcgct
     5761 ggtgttgcgt acgggtttgg agcacgcgtc tccgcacttg aagcggcacg tcacatgctg
     5821 ccgtttggac tttccgtcat gggtaacgaa caggttcaac ggcacaagcg ccatgggtgc
     5881 aggtccttcc gacacgaact ccggccgagg gctgccggcc ggacgcgccg aaatctacgt
     5941 cagtttggca atcagctggg aagcaattgg taaacaactg aggacgctag gcgtccagca
     6001 cgccacgcag aatgtcgaca agtgcccgcg gctggtcgcc ctgtacggag tgccccgagt
     6061 cggcgacgat gtgggtgcgc tggaaaccgg gagcgccctt ggcgaaggcg tcggcgtcct
     6121 cgtcgttgac gaagaacgag ttcgcgccgc gcaccagggt ggtcggcatg gtgatcgcgg
     6181 gcacgtcctc ccacagaccc gtaaaggaat ccggcatctc cttgttcttg cttcggaagg
     6241 tgtcgtagcg ccacgtccag gtgccgtcgt cgttgtgctt cgaattgtgg aaaacgcccc
     6301 gccgcaacga cttccggtcg cggtgcggtg aggcctgaac ggtcacatcg agcatggcct
     6361 caaacgaggg aaaggtgcga tcgccctgca cgagcgcgac ggtgcccatc tgcgccttgg
     6421 tcatctgctc gtggcgctcc ggcgctgacg gggtgacgtc gacgagcagc agttcgggaa
     6481 caagcgccgg ttcggtggcg gcgatgcgca gtgcggtcaa tccgccgagc gacatgccga
     6541 cgacgagccg cggatgcggc gcccagtccc gcaggacggg ccgcaatgtg tcggcgttga
     6601 gcctcggccc gtagtcgccg tcctcccgcc acgccgaccg cccatgcccg ggcaggtcga
     6661 ttgccagcgc gggcacatcg aggccgagga tgacggtgtc ccaggtgtgt gcgttctggc
     6721 cgccgccgtg caggaacacc acctcgggag gctcgtcgcc ccatttcaat gcgctgatat
     6781 cgcccgattc gacgcgggcg accgaaggca gtggtccggt gtggccgatc tgttcggcat
     6841 tttccggtag cagcgcgaac tcgtcgacat tcaccagttc gtcatcgggg atttccgcca
     6901 cggctcgaca ttacgcaccc ccgaaattcg ggtgccatcg gagcgacatt gtgtcagcgt
     6961 cgagtgcatg gttcccaaga cgcacgctcc gaaaggattg acgctccgca cggccgagga
     7021 cgcggactgg cccgcgatgg cgctgctggc ggcgacgtgt ttcggttcct ggcgtccgga
     7081 ggagtcgaac gaggcgtggc ggaccatgat gcccgtggac ggcggcgtcg tcgcgtgcga
     7141 cgggcccgac gtcgtcggtg tggcgctgta cctggacttg gaactcacgg tgccgggtgg
     7201 cgccgtgctg ccgatggccg gcgtttcctg ggtggcggtt tccccgacac atcgtcg
//
LOCUS       DASYLX010000016         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2305334,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000016 DASYLX010000000
VERSION     DASYLX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305334"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VGP27_00480"
     CDS             complement(<1..790)
                     /locus_tag="VGP27_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7849825.1"
                     /translation="MRVLEGSNRVRGGVMGILILLLVIGVGQSFTSVPMLFAQPTYYA
                     YFSDTGGLSGGDKVRIAGVDVGQVKSFEIEGDKVKIGYSLSGTQIGTDSRAAIRTDTI
                     LGRKNLEISPRGNTALQANAVLPLGQTTTPYQIYDAFFDLTKAASGWDTETVKRSLNV
                     LSETIDQTYPHLSAALDGVARFSDTIGKRDEDVRKLLGNANKIAGVLGNRSEQINALL
                     TNAQTLLAAVNERSYAVSMLLERVGQFSAQVAGFVNDNPNLNHVL"
     gene            complement(787..1818)
                     /locus_tag="VGP27_00485"
     CDS             complement(787..1818)
                     /locus_tag="VGP27_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HEV7849826.1"
                     /translation="MKITGTAIKLGIFALVLLLFTAIIIVVFGQMRFDRTTGYSAIFS
                     DASGLRAGEFVRASGVEVGKVSKVEIRDGGRVAEVHFAVERDLQLFRGTTASIRYLNL
                     IGDRYLELKRGDNDQRLPGGSVIPIERTEPALDLDALIGGFRPLFRALNPDKVNNIAQ
                     SIITIFQGEGGTINNILDQTGQLTSALADRDQAIGEVIRNLNNVLDTTVKHQKQFDDT
                     VVNFEKLITGLKNRADPIASSVADISDAAGTVGDLLADNRPLLQSTISNLEIIQQPLV
                     DQRDQLNDLLTRLPTALKIIGRAGGVYGDFFNFYACDVTLKLPGLQPGGPVRTVKVWS
                     QPTGRCTPQ"
     gene            complement(1815..3083)
                     /locus_tag="VGP27_00490"
     CDS             complement(1815..3083)
                     /locus_tag="VGP27_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7849827.1"
                     /translation="MTAPLNQSRTPPYKLAGAVLTLLTVVAVVLVYLQFRGDFLPREQ
                     VTMMSSRAGLSMDPGSKVTYNGVEIGRVADVTQTAVGDQPQAKITLEIDKKYLDLIPR
                     NVDAKISATTVFGNKYISFTSPKNPSPQRITSSDVIDATHVTTEFNTLFETVTSVSEK
                     VDPIKLNQTLTATAQALQGLGDRFGQSIINGNKILGDLNPRMPQIRRDNQLLADLGDV
                     YANAAPDLFDGLENAVTTARTLNEQQGNIDQALMASIGFGNTGAEIFEKGGPYLIRGA
                     EDLIPTSQLLDEYSPALFCTIRNFHDVEPKIAASLGGNGYSLNTRTLIVGAPNAYVYP
                     DNLPRVNAHGGPEGRPGCWQPVTRDLWPSPYLVMDTGASIAPYNHLEVASPFGCALCV
                     IPILPAPIPVLFPGSEYIWGRQLGEFTINP"
     gene            complement(3087..>3308)
                     /locus_tag="VGP27_00495"
     CDS             complement(3087..>3308)
                     /locus_tag="VGP27_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7849828.1"
                     /translation="PDDVFWSFVQAIIISVIVMLNHCYYGYFASGGPVGVGEAVGRSM
                     RASLVAIVCVVLFASLALYGVDPNFNLTV"
BASE COUNT          570 a         1016 c         1103 g          619 t
ORIGIN      
        1 cgaggacatg gttcaggttc gggttgtcgt tgacgaagcc cgcgacctga gccgagaact
       61 ggcccacccg ctccagcagc atgctgaccg cgtagctgcg ctcgttgacc gcggcgagca
      121 gggtctgtgc attcgtcagc agagcgttga tctgttcgct gcggttgccg agcacgcccg
      181 cgatcttgtt ggcgttgccc agcaacttcc ggacgtcctc atcgcgcttg ccgatggtgt
      241 cggagaaccg ggccacaccg tcgagggcgg cactcaggtg cgggtaggtc tgatcgatcg
      301 tctccgagag cacattcagc gaccgcttga ccgtctcggt gtcccagccc gacgccgcct
      361 tcgtcaggtc gaagaaggcg tcgtagatct ggtacggcgt ggtcgtctga ccgagcggca
      421 ggacggcgtt tgcctgcagc gccgtatttc cgcgcggcga aatctccagg ttcttgcggc
      481 ccaggatggt gtcggtgcgg atggcggccc ggctgtcggt gccgatctgc gtgccgctca
      541 gcgagtagcc gatcttgacc ttgtcaccct cgatctcgaa cgacttcacc tggccgacat
      601 cgacacccgc gatgcggacc ttgtctccgc cgctgagtcc gccggtgtcg gagaagtagg
      661 cgtagtaggt gggttgggcg aacagcatcg gcacgctcgt gaagctctgc cccaccccga
      721 tcacgagcaa caggatgagg atgcccatca ccccgccgcg gacgcggttg ctaccctcaa
      781 ggactctcat tgcggcgtgc acctaccagt gggctgcgac cagaccttca ccgtgcgcac
      841 cgggccaccc ggctgcaggc cgggcagctt gagcgtgacg tcgcaggcgt agaagttgaa
      901 gaagtcgcca tagacgccgc ctgcgcggcc gatgatcttc agcgccgtcg gaagcctggt
      961 caacagatcg ttcagctggt cccgctggtc gacgagcggc tgctggatga tctccaagtt
     1021 gctgatcgtg ctctgcagta ggggacggtt atcggcgagg agatctccga cggtgcccgc
     1081 cgcgtcgctg atatcggcga cggagctggc aatcgggtcg gcccggttct tcaggccggt
     1141 gatcagtttc tcgaagttga cgacggtgtc atcgaactgc ttctggtgct tgacggtggt
     1201 gtcgagcacg ttgttcaggt tgcggatcac ctcgccgatg gcctggtcac ggtcggcaag
     1261 cgcggacgtg agctgccccg tctgatccag gatgttgttg atcgtgccgc cctcgccctg
     1321 gaagatcgtg atgatggact gagcgatgtt gttgaccttg tcgggattca gcgcgcggaa
     1381 caacggccgg aagccgccga tcagtgcgtc caggtcgagg gcgggctcgg tgcgctcgat
     1441 cggaatgacc gaacccccgg gcagccgctg atcgttgtcg ccgcgcttga gttccaggta
     1501 gcggtcgccg atcaggttca gataacggat cgatgccgtc gtcccccgga acagttgcag
     1561 gtcgcgttcg acggcgaaat gaacctcggc gacgcgtcca ccgtcccgga tctcgacctt
     1621 ggagaccttc ccgacctcaa ccccggacgc ccggacgaac tcgccggccc gcagaccgct
     1681 ggcatcgctg aagatggccg agtagccggt cgtgcggtcg aaccgcatct gaccgaacac
     1741 cacgatgatg atcgcagtga acagcaacag caccagggcg aagatgccga gcttgatggc
     1801 cgtaccggtg attttcatgg gttgatcgtg aactccccga gctgacggcc ccagatgtat
     1861 tcgctgcccg ggaacagaac cggtatcggc gctgggagga tgggaatgac gcaaagcgcg
     1921 caaccgaacg gcgatgcgac ctcgaggtgg ttgtacgggg cgatcgaggc gccggtgtcc
     1981 atcaccagat acgggctcgg ccacagatca cgggtaacgg gctgccaaca cccgggcctg
     2041 ccttccggcc cgccgtgcgc gttcacccga ggcaggttgt ccggatagac ataagcgtta
     2101 ggtgctccga cgatcagcgt gcgggtgttc agcgaataac cgttgccgcc gagcgacgct
     2161 gcgatcttgg gctcgacgtc gtggaagttg cggatcgtgc agaacagcgc ggggctgtac
     2221 tcgtcgagca gctgcgacgt cggaatcagg tcctcggcgc cgcggatcag gtacgggccg
     2281 cccttctcga agatctcggc gccggtgttg ccgaatccga tcgaggccat caacgcctgg
     2341 tcgatgttgc cctgttgttc gttgagggtg cgcgcggtcg tgacggcgtt ctcgaggccg
     2401 tcgaacagat ccggcgccgc gttggcgtag acatcgccga gatcggccag cagttggttg
     2461 tcccgacgga tctgcggcat ccgcgggttc aggtcgccga ggatcttgtt gccgttgatg
     2521 atcgactggc cgaaccggtc gccgagcccc tgcagcgcct gcgcggtcgc ggtgagtgtc
     2581 tggttcagct tgatcgggtc gaccttctcc gacaccgagg tcaccgtctc gaacagcgtg
     2641 ttgaactcgg tggtcacatg cgtcgcgtcg atgacgtccg acgacgtgat ccgttggggc
     2701 gacggattct tcggcgacgt gaaggagatg tacttgttgc cgaacaccgt ggtggcgctg
     2761 atcttggcgt ccacgttcct cgggatcagg tcgaggtact tcttgtcgat ctcgagggtg
     2821 atcttcgcct gcggctggtc gccgactgcg gtctgggtga catcggccac ccggccgatc
     2881 tcgacaccgt tgtacgtcac cttcgagccg gggtccatcg aaaggcccgc gcgactcgac
     2941 atcatcgtca cctgctcgcg cggcaggaaa tcgccgcgga actggagata caccagcacg
     3001 acggcgacga ccgtcagcag cgtcaagacc gcaccggcca acttgtacgg cggtgtgcgc
     3061 gactggttca ggggtgccgt catgccctac accgtgaggt tgaagttcgg gtcgacgccg
     3121 tagagcgcca acgaagcgaa caggacaaca cacacgatgg ccaccaacga ggcgcgcatc
     3181 gaccggccga cggcctcgcc gacgccgacc ggtccgccgc tggcgaagta gccgtaatag
     3241 cagtggttga gcatgacgat caccgagatg atgatcgcct gcacgaagga ccagaagacg
     3301 tcgtccgg
//
LOCUS       DASYLX010000017         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3840988,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000017 DASYLX010000000
VERSION     DASYLX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3840988"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGP27_00500"
     CDS             <1..431
                     /locus_tag="VGP27_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849829.1"
                     /translation="TSTRMSRAFLATPIIAAGLLVSPAVGHANVDCPTGWEFGPSLRL
                     LQSDGWTVTTATAGRSVGGSAVALPPNKGVLWRGAGEGGSDGTTVAFGIGWDNGTTMH
                     YTGVIDQSTGALTGARPDGVTWKSAANMQCIGGADTPPPG"
     gene            complement(479..655)
                     /locus_tag="VGP27_00505"
     CDS             complement(479..655)
                     /locus_tag="VGP27_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849830.1"
                     /translation="MKKFGLAGITGFAAAGLAAAILGFATPAQADVVHHDWIHDIQPT
                     VNVPQVDMTVQQSR"
     gene            789..1220
                     /locus_tag="VGP27_00510"
     CDS             789..1220
                     /locus_tag="VGP27_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7849831.1"
                     /translation="MAKLELSRSLSMTPADAWAHVSDFSTLGDWLKMHQGWRSELPDE
                     LELGTTLVGVAGVKGMRNRVTWTVKELDAPKLLAVTGEGVGGTKYGLRMTVKPTESGC
                     DFTVKIDLGGRPLFGPIGATAARLVKGDIERSIERFSELYS"
     gene            1283..1750
                     /locus_tag="VGP27_00515"
     CDS             1283..1750
                     /locus_tag="VGP27_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7849832.1"
                     /translation="MSVEPIPEGYTSLTPFLCVEGASAAIDFYVAVFGAELVDRMDGP
                     NGTVAHAELDFGNGRLQLSDPNEAYQIAAADPAGFATHSVALYCGDTDAVVARAEKAG
                     ATIREPAQTFVTGDRFASILDPFGQRWTVMTRVEDVSADERDRRMAEWARQNA"
     gene            complement(1866..3098)
                     /locus_tag="VGP27_00520"
     CDS             complement(1866..3098)
                     /locus_tag="VGP27_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7849833.1"
                     /translation="MVRKRSRTGAVVAAAAAVAVVSGGLGAAVLANRSDPAAATSAAT
                     AATAAAPAPNQPVAAPPVGSVEQVAAKVMPSVVKLQITMGQGAEEGSGIVLSSDGLIL
                     TNNHVVAAVSSAAADRQALGGQGQDYQGSPGSQAPDGPGDPPSFGNGGFSSQDPEPTV
                     AFSDGKTVPFTVVGTDPADDIAVVRAQGVSGLTPITMGSSKDLKVGQNVVAIGSPLGL
                     EGTVTTGIISALDRPVATGDGSNGQHSVMNAIQTDAAINPGNSGGALVNMNGELVGVN
                     SAIASMGGGSDSQGAQSGSIGLGFAIPSDQAKRIADELVSTGSATHASLGIQLTQDPG
                     SHGAAVAQVVDGGPAAAAGLPGGVVITKVDDRPIDGADALVAAVRSKAPGDNVTLTYT
                     DASGAAQTVRVTLGQSQT"
     gene            3429..3866
                     /locus_tag="VGP27_00525"
     CDS             3429..3866
                     /locus_tag="VGP27_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV7849834.1"
                     /translation="MSCVFCAIVAGEAPAIRIYEDDGYVAILDIRPFTRGHTLVIPKR
                     HTVDLTDTPPETCADLVRIGQRISKAARRSGLHADGNNVVINDGKAAFQSVFHIHLHV
                     LPRQSGDKLSFAKGMLLRRDPDREETARILRDALAELDATQQD"
     gene            3872..4309
                     /locus_tag="VGP27_00530"
     CDS             3872..4309
                     /locus_tag="VGP27_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7849835.1"
                     /translation="MASLIQKAFGTYLRLHDTVYQRTNGWIGHRLMLLPSLLLHTVGA
                     KTGKSRTTSLTYARDGDDYLVVASNGGASRSPGWYHNLKADPDIEINVGPRRFAVTAQ
                     PVLPGDPNRERLWKIVTKFNKSYNEYQKKTRRPIPIVRLTPKA"
     gene            complement(4310..>5242)
                     /locus_tag="VGP27_00535"
     CDS             complement(4310..>5242)
                     /locus_tag="VGP27_00535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7849836.1"
                     /translation="YTRYWVAEHHNMPSVAATSPPVLIAHLAANTSHIRLGSGGVMLP
                     NHAPLAVAEQFALLEAAHPGRIDLGIGRAPGSDPLTSYLLRNAAGRDDSDIDRFPEYL
                     DHVAAMMSASGVHVSLPQQDYILKATPAATSEPRLWLLGSSMYSARLAAAKGLPYVFA
                     HHFSGQGTAEALAVYRSEFQPSDLAAEPVTFLTVNAVVAESHDEAMALLLPNLQMMAK
                     LRTGQPLGPVDLVEDAEQNRLTSQESAVVESGLRRAVVGDPVEAAQQVRALAEEFGVD
                     EVMVHPVASARRGTDPATAPARDNTLELLAKELF"
BASE COUNT          901 a         1731 c         1750 g          860 t
ORIGIN      
        1 taacctctac ccgtatgtca cgcgcattct tggcgacgcc gataatcgcc gccggtctcc
       61 tggtatcccc tgctgtcggc cacgcgaacg tcgactgccc aaccggatgg gagttcgggc
      121 cgtccttgag gctgctgcag agcgatggct ggacggtgac gaccgcgacc gcagggcgca
      181 gtgtcggcgg ctccgccgtc gccctcccac ccaacaaagg cgtgttgtgg cgcggggccg
      241 gcgagggcgg cagcgacggc acgaccgttg ccttcggcat cggctgggac aacggaacca
      301 cgatgcacta caccggcgtg atcgatcaga gcaccggtgc attgaccggt gcacgtcccg
      361 atggtgtgac gtggaagagc gccgcgaaca tgcagtgcat cggcggggcg gacaccccac
      421 ccccgggctg aaacaaaaga gggcacccga caccgggtgc cctcctcgcg tgtcttcgtc
      481 agcggctctg ctgaacggtc atgtcgacct gaggcacgtt caccgtcggc tggatgtcgt
      541 gaatccagtc gtggtggacg acgtcggcct gggccggagt ggcgaagccg aggatcgcgg
      601 cggcgaggcc ggctgcggcg aacccggtga tccctgcaag tccgaacttc ttcatctcag
      661 tgccttcttc gtatctctgt gggctcttgt cggtgcccta tgacgtgcta aaccaacagg
      721 tcagacggtt aattccggtg gcagaaattg atcggcagct ggcaggaaac gatggttacg
      781 attcaccagt ggcaaaactc gagctgtccc gcagcctttc gatgacaccg gccgacgcct
      841 gggcacacgt gtctgacttc tcgaccctgg gcgactggct caagatgcat cagggctggc
      901 gcagtgagct gccggacgag cttgaactcg gcaccacgct ggtgggtgtc gcaggagtca
      961 aggggatgcg caaccgggtc acctggacgg tcaaggaact cgacgcgccg aagctgttgg
     1021 cggtgaccgg cgagggcgtc ggcggcacga agtacggact gcggatgacg gtcaaaccca
     1081 ccgaatcggg atgcgacttc accgtgaaga tcgacctcgg cggacggccg ctgttcgggc
     1141 ccatcggcgc caccgccgcc cggctggtca agggagacat cgagcgatcg atcgagcgat
     1201 tctccgagct ctacagctga agcgcggcgg ctggcgcgaa attccaatcc cgcaccggcc
     1261 acggttggcc acgctggcgg acatgagtgt tgaaccgata cctgagggct acaccagcct
     1321 gaccccgttc ctgtgcgtcg agggcgcctc cgccgcgatc gacttctacg tcgcggtgtt
     1381 cggtgccgag ctcgtcgacc ggatggacgg cccgaacggc acggtcgccc atgccgagct
     1441 ggacttcggc aacggccggc tacagctgtc cgacccgaac gaggcctacc agatcgccgc
     1501 cgccgacccg gcggggttcg cgacccactc ggtggcgctg tactgcggcg acaccgacgc
     1561 cgtggtggcg cgcgccgaga aggcaggcgc gacgatccgc gaacctgcgc agaccttcgt
     1621 cacgggcgac cgcttcgcgt cgatcctcga tccgttcggc cagcgctgga ccgtgatgac
     1681 gcgcgtcgag gacgtctcgg ccgacgaacg cgaccgccga atggccgagt gggccaggca
     1741 gaacgcctga cgacgaaggc ggcgagggac gtgccgacga ggagtcgggc gcgacgacaa
     1801 cgcctgacga cgaaggcggc gaggagtcgg gcgcgaggag aacgcctagc ccacccggct
     1861 accggttacg tctgcgactg gccgagggtc acccgcacgg tctgagcggc gccggacgcg
     1921 tcggtgtacg tcagcgtcac gttgtctccc ggtgccttcg agcgcaccgc ggcgacaagt
     1981 gcatcggcac cgtcgatcgg ccggtcgtcg accttggtga tcaccacacc gcccggcagc
     2041 cccgcagccg cggccggacc gccgtcgacc acctgcgcga cggcggcccc gtgcgagccg
     2101 ggatcctgcg tcagctgaat gcccaacgac gcgtgcgtcg cgctgcccgt cgacaccagt
     2161 tcgtcggcga tgcgcttggc ctgatcggat gggatcgcga aacccagacc gatcgaaccg
     2221 ctctgcgcgc cttgggagtc cgagccgcca cccatcgatg cgatcgccga gttcactccg
     2281 acgagttcac cgttcatatt caccagcgct ccgccggaat tgccggggtt gatggcggcg
     2341 tcggtctgta tcgcgttcat caccgaatgc tggccgttgc tcccatctcc ggtggccacg
     2401 ggacggtcca gcgcgctgat gatgcccgtc gtcacggtgc cttccagccc gagcggcgag
     2461 ccgatcgcca ccacattctg gccgaccttc aggtccttcg acgatcccat cgtgatcggg
     2521 gtcagccccg aaacaccctg cgcccgaacg acggcgatgt catcggccgg atcggtgccg
     2581 acaacggtga acggtacggt cttgccgtcg gagaaggcca ccgttggctc ggggtcctgg
     2641 ctagagaatc cgccgttgcc gaaggacggc gggtcgcccg gcccatccgg tgcctggcta
     2701 cccggagagc cttgatagtc ctgtccttgt ccgcccagcg cctgccggtc agcggcagcg
     2761 ctgctcaccg cggccacgac gtggttgttg gtcaggatca gaccgtctga gctcagtacg
     2821 atgccagagc cctcctcggc gccctgcccc atcgtgatct gcagcttcac cacgctgggc
     2881 atcaccttcg cggcaacctg ctcgaccgag ccgacaggcg gtgccgcgac gggctggttg
     2941 ggggccggcg ccgctgcggt ggcggcggtg gcggcgctgg tggcggcggc ggggtcagag
     3001 cggttggcca gcaccgctgc gccgagaccg ccggacacca cggccacagc cgcggctgca
     3061 gccacgacgg cgccggttcg agatcgcttg cgtaccatga atatcggtgc cgtcggagcc
     3121 gaatcagatt gataggcagt gccatcggga ctgtacggcg gcggctgctg cgttgcgtaa
     3181 cgccagtcgt agggctgctg ataatctccc tggggcacgc ccgaatattg ttgcctcaca
     3241 ggctggcccg ggcgtgatgc acggccggat cgacgcagcg atgaccgcga ttgctttttc
     3301 atggcgtggt tcttccctta acagtgggga gcgaaatggt tagcctcact gtgcactcgt
     3361 tcactgagac tggactgaga gaaggatcta tacagtccaa gacttcggtc acgagttagc
     3421 ctggccggat gtcatgtgtg ttctgtgcca tagtcgcggg tgaggccccc gccatccgga
     3481 tctatgaaga cgacggctac gtggccatcc tggacatccg tccgttcacc cgcggccaca
     3541 cgctcgtcat ccccaagcgt cacaccgtcg acttgaccga cacgccgccc gagacctgcg
     3601 cagaccttgt ccggatcggg cagcgcatct cgaaagcggc gcgccggtcc gggttgcatg
     3661 ccgacggcaa caacgtcgtc ataaatgacg gcaaggccgc atttcagtcc gtgttccaca
     3721 tccacctgca tgtgctgccc cggcaatccg gtgacaagct gtcgttcgcc aagggcatgc
     3781 tgctgcgccg cgatcccgat cgcgaggaga ccgccagaat cctgcgagac gcgctggctg
     3841 aactcgacgc gacgcagcag gactgacgga catggcttcc ctcattcaga aggcgttcgg
     3901 cacctatctg cggttgcacg acaccgtcta tcagcggacc aacggctgga tcggccatcg
     3961 gctgatgctg ctgccgagcc tgctgttgca caccgtcgga gccaagaccg gcaagtcccg
     4021 taccacgtcg ctgacctacg cgcgtgatgg agacgactat ctggtggtcg catcaaacgg
     4081 cggcgcgtcg cggtcgccgg gctggtacca caacctgaag gctgatcccg atatcgaaat
     4141 caacgtgggt ccaaggcgtt tcgcagtaac ggcgcaaccc gtcctgcccg gcgacccgaa
     4201 ccgcgagcgg ctgtggaaga tcgtcaccaa gttcaacaag tcgtacaacg agtatcagaa
     4261 gaagaccagg cgcccgatcc cgatcgtcag gctgacgcca aaagcgtgat cagaacagct
     4321 ctttggcgag cagttcgagt gtgttgtcgc gtgccggtgc tgtcgcggga tcggtaccgc
     4381 ggcgagccga ggccaccgga tgcaccatca cctcgtcgac cccgaactcc tccgccagtg
     4441 cacgcacctg ctgcgcggcc tcgaccggat ccccgacgac ggctcgacgc agaccggact
     4501 cgacgaccgc cgactcctga gatgtcagcc ggttctgttc ggcgtcctcg acgagatcga
     4561 ccggcccgag cggctggcca gtgcgcaact tcgccatcat ctgcaggttc ggcagcagca
     4621 acgccattgc ctcgtcgtgt gattcggcca cgaccgcgtt gacggtcagg aacgttacgg
     4681 gctcggccgc cagatcgctg ggctggaact ccgatcggta gacggccagc gcctcggcgg
     4741 tgccctgccc cgagaagtga tgcgcgaaca cgtacggaag ccccttggcc gcggcgaggc
     4801 gcgcggagta catcgacgag ccgagcagcc acagtcgcgg ctcgctggtc gcggcgggcg
     4861 tcgccttgag gatgtagtcc tgctgcggga gggagacgtg cacaccgctg gcgctcatca
     4921 tcgcggcgac gtggtccaga tactccggga accggtcgat gtcgctatcg tcgcgtcccg
     4981 ccgcattgcg gagcagatac gacgtcagag ggtcggagcc cggcgcgcgg ccgataccga
     5041 ggtcgatgcg gccgggatgg gccgcctcga ggagcgcgaa ctgttcggcg acggccagcg
     5101 gcgcgtggtt cggcagcatc acaccgccgg agccgaggcg aatatgcgag gtgttggccg
     5161 ccaggtgggc gatcagcacc ggcgggctgg tggccgccac cgacggcatg ttgtggtgtt
     5221 cggcgaccca gtagcgggtg ta
//
LOCUS       DASYLX010000018         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2963351,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000018 DASYLX010000000
VERSION     DASYLX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963351"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VGP27_00540"
     CDS             complement(<1..1076)
                     /locus_tag="VGP27_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7849837.1"
                     /translation="MRLTWPLVGREEEMRLIDAAIFDLDLSGVVISGAAGVGKSRLAR
                     EALMVAAAGGCDVRWVVGTSCGRGIPLGALASWPGLIGDDSLQLVCGVLDSLAAASSV
                     APVVIGVDDPHLLDDLSMFVLDQIVARGVAKVLLTLRNDEPIPAAIQELWKLGQFERV
                     ELEPLSLEVTTSLLSTTLVGSMDPETAGSLWQLTHGNALYLRHIVDQAVVDGRLGKRS
                     GYWQWVEDPVVPHGLVELIESRIGALPESVSAVVDALAVGEPVELAALRRIAWPEAVE
                     DADVRGLIRLDALDDRVQVRLAHPLYGEVRRRRAPATRLRRLRGLLAVELAGADDRDN
                     VRVLVRRGALSLDSDLAPDADLLLR"
     gene            1326..>2536
                     /locus_tag="VGP27_00545"
     CDS             1326..>2536
                     /locus_tag="VGP27_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849838.1"
                     /translation="MVTATGSPLTLDAHASRAWMRRFARTTPGLVVAIAIAVAACCVV
                     AGVVCAAALNGRIAEHKAVLDHSEPFAYAAQNLYAASSAADAAAATAFLSGGVQTPLM
                     RARYEQALAAAASALADATAGAPDAETRKALAEVSAQLATYTGLVEAARANNVQGFPI
                     GSAYLREASSSMQTKLLPGAEKIYSADLARVDHAQRRVGSLPMVGLALLLITLAAIGV
                     GSVIMANRTNRRFNLGLVAAAVLIMVLIASMVGAVRLAAGDIERGRTEGTARFGQLAE
                     ARILAQQARTDETLQLITRGDITAGEKSFDGHVDTLLARIGTASPAATEAVQKWVASH
                     RKQVDAYRAGDYPGAVTQALSTDYGGSGAQFAVVETSLRDAIEQARTTMRDHVADAGR
                     YLVWLPAVTLV"
BASE COUNT          437 a          956 c          793 g          350 t
ORIGIN      
        1 cgaagcaaaa gatcggcgtc gggcgccaag tcggagtcca ggctcaacgc cccccgacgc
       61 accagcaccc ggacgttgtc gcgatcgtcc gcgcccgcca actcgaccgc cagcagtccc
      121 cgcagtcgcc gcagcctcgt agcaggcgcc cgtcgcctcc gcacctcgcc atacaacggg
      181 tgcgccagcc gaacctggac ccgatcgtcg agcgcatcga gcctgatcag gccacggacg
      241 tcggcgtcct cgaccgcctc cggccaggcg atccgccgca acgccgccaa ctcaaccggc
      301 tcaccgaccg caagcgcgtc gacgacggcg ctgactgact cgggcagcgc gccgatccgc
      361 gactcgatca gctcgaccag gccgtgcggc accaccgggt cctcgaccca ctgccagtac
      421 ccgctgcgct tgcccagccg gccgtcgacg accgcctgat cgacgatgtg ccgcagatag
      481 agcgcgttgc cgtgcgtcag ctgccacagg ctgcccgccg tctcgggatc catcgagccg
      541 accaacgtcg tcgacagcag cgacgtcgtc acctccaacg acagcggttc cagctcgaca
      601 cgctcgaact gtccgagctt ccaaagctcc tgtatcgcag ccggaatcgg ctcgtcatta
      661 cggagcgtca gcagtacctt cgccacgcct cgcgccacga tctggtcgag cacgaacatc
      721 gacaggtcgt cgagcaggtg cggatcgtcg acgccgatca ctaccggggc taccgacgag
      781 gcggccgcaa gcgaatccag aaccccgcac accagctgca ggctgtcgtc gccgatcaac
      841 cccggccacg acgccagcgc accgagcggg atgccgcgcc cgcacgacgt gccgaccacc
      901 caccggacat cgcagccgcc agccgccgca accatcagcg cctctcgcgc gagacggctc
      961 ttcccgacgc cggccgcgcc gctgatcacg acgccggaca ggtccaggtc gaagatcgcg
     1021 gcgtcaataa ggcgcatctc ctcctcgcgc ccgactaacg gccacgtcaa ccgcatccgc
     1081 tcagattaaa agaccaggta ggaggtgcgc accgtaacga acgggccgac cgccggtcat
     1141 ctaagtagtc cagatcgccg acaacggccg aatccgagta gtcgactact ctcctcaccc
     1201 ccgctgtgca gcgcgaatat cgctgtaacc agtacgtcaa ttgcaccgat accgcccgaa
     1261 cgggagaccg agatgagcaa tcattggctt gctcctcacc tcacggggac gaaacgggtg
     1321 ggcaagtggt gacggcaacg ggctctcctc tcacgctgga cgcgcacgcg tcccgcgcgt
     1381 ggatgcgccg gttcgcccgc acgacacccg gattggtcgt cgcgatcgcc atcgccgtcg
     1441 cggcctgctg cgtcgtcgcc ggagtcgtct gcgccgccgc gctgaacggc cggatcgccg
     1501 agcacaaggc ggtgctcgac cactcggagc cgttcgccta cgccgcccag aacctctatg
     1561 ccgcgtcgtc tgcggccgac gccgccgctg cgacggcgtt cctgtccggc ggggtccaga
     1621 cgcctttgat gcgggcgcgg tacgaacagg ctctggccgc ggcggcatcg gcgctcgcgg
     1681 acgcgaccgc cggtgcgccg gacgccgaaa cccgcaaggc gctcgccgag gtgtccgcgc
     1741 aactggccac ctacaccggt ctcgtcgagg cggcccgcgc gaacaacgtg cagggcttcc
     1801 caatcgggtc cgcctacctg cgcgaggcgt cgtcgtcgat gcagaccaag ctgctgcccg
     1861 gtgccgagaa gatctacagc gccgatctcg cccgcgtcga ccatgcccaa cgccgcgtcg
     1921 gatcgcttcc catggtgggg ctcgccttgc tgctgatcac cctggccgca atcggtgtcg
     1981 gctcggtgat catggccaac cgcacgaaca ggcggttcaa cctcgggctg gtcgccgcag
     2041 cggttctcat catggtcctg atcgcctcga tggtcggggc ggtgcgcctg gcggcaggcg
     2101 acatcgagcg cggccgcacc gagggcaccg cgagattcgg gcaactcgcc gaggcgcgca
     2161 tcctcgccca acaggcccgc accgacgaaa cgctgcagct gatcacccgc ggcgacatca
     2221 ccgcaggcga gaagtcgttc gacgggcacg tcgacacgtt gctcgcccgc attgggacgg
     2281 cgtcgcctgc cgcgaccgag gccgtgcaga agtgggtcgc cagccaccgc aagcaggtgg
     2341 atgcctaccg cgccggcgac tatccgggtg cggtcaccca ggcgctcagc acggactacg
     2401 gaggttcggg cgcgcagttc gccgtcgtcg agacgagcct gcgcgacgcc atcgagcagg
     2461 cccggaccac catgcgcgac cacgtcgccg acgcgggccg ctatctggtg tggttaccgg
     2521 cggtgaccct ggtgtt
//
LOCUS       DASYLX010000019        11240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_330490,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000019 DASYLX010000000
VERSION     DASYLX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330490"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGP27_00550"
     CDS             <1..585
                     /locus_tag="VGP27_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEV7849839.1"
                     /translation="SALWFALDTDLPVIVDADGLTILAAHPDLIVNRKAPTVLTPHAG
                     EFARLASGAGLPTTSDQAGPGDDRIAATRRLSDQFGATVLLKGNVTVIAEPGGPVYLN
                     PAGQSWAATAGSGDVLSGIIGALLATGLPPGEAAAAAAFVHARAANLSAADPGPQPAP
                     TSASGILAHVRTAIARLQQSGKDSAWPRGTPKAQ"
     gene            555..1946
                     /locus_tag="VGP27_00555"
     CDS             555..1946
                     /locus_tag="VGP27_00555"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEV7849840.1"
                     /translation="MAPRHTQSPVIAPAYTGRMSMAPIPSLRMPDESMNPDAAYRFIH
                     DELMLDGSSRLNLATFVTTWMDPQAEKLMAETFDKNMIDKDEYPATAAIESRCVSMVA
                     DLFHAEGLRDDDPSSATGVSTVGSSEAVMLAGLALSWRWRERVGNGKDGWRSRRPNLV
                     MGSNVQVVWEKFCRYFDVEPRYLPMEKGRYVITPEQVLENVDEDTIGVVAILGTTFTG
                     ELEPIGEICEALDNLAAGGGLDIPVHVDAASGGFVVPFLHPDMVWDFRLPRVVSINVS
                     GHKYGLTYPGIGFVVWRSPEHLPEDLVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNF
                     LRLGREGYSQVMECLSQTARWLGDQLRESEHFELISDGSAIPVVSFRLKGDPAYTEFD
                     VSEALRSFGWQVPAYTMPENATDIAVLRVVVREGFSMNLARALRDDAVTALKHLDELK
                     PDGHFNELQPFAH"
     gene            1957..3735
                     /locus_tag="VGP27_00560"
     CDS             1957..3735
                     /locus_tag="VGP27_00560"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7849841.1"
                     /translation="MKDPDAAFDVEALRDRYARERGRRLRPDGIAQYVEIAGPFAGFA
                     EDPWSEPHFTRAPLQDEVDVAIVGAGFGGLLIGARLRELGVQSIRIIDKAADVGGTWY
                     WNRYPGIACDVESYVYMPLLEELGYLPTEKYAKGDEILAHCRRIAEHYDLYRDACLQT
                     EVTEIRWDGRLSRWIVATNHGDAIQARFVAMANGYLQKPKLPGIEGIADFRGHAFHTS
                     RWDYGYTGRHLAKLADKRVGIIGTGATAIQCVPHLGRSVEHLFVFQRTPSTVDVRGNQ
                     QTDPEWSAELKNGWQRHRIENFQLLTAGGEADEDLVGDAWTSIVKKLFVMRQRSVAGM
                     SDEDRLRAVEMADFTKMEEIRARVDSVVSDRATAEGLKPWYGYFCKRPCFHDEYLQTF
                     NRDNVTLVDTRGLGVQRITPGGVVVDGTEHVVDCLIFATGFEVGTDYSRRTGFELVGR
                     DGLALTDKWRDGVRTLHGLSVRGFPNCFVLSIAQSGFTVNFPYLLDVQATHTAWMIAW
                     ALRNAVDSLEASAEAESAWVGTVVERSSASLARAKACTPGYYNREGQANAKTRQGSFF
                     FGSPTEYADILAASRANGSPEGFEFS"
     gene            3732..5231
                     /locus_tag="VGP27_00565"
     CDS             3732..5231
                     /locus_tag="VGP27_00565"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7849842.1"
                     /translation="MTRRYLVGRLSALTRHRRSPRVVIIGAGFGGLAAAVALRRKGID
                     DLTIIERADGVGGTWRRNVYPGAACDIQSHLYSLSFAPNRAWTRTYAYQPEILAYLES
                     VADEFDLRRHLVLNTEVRTMRWDADASHWDVELAGGHTVVADVVVSAVGLFGSVRYPD
                     IDGVAGFSGTLMHTAQWDPTVDLTGKRVAVIGTGASGVQVVPELAEIAGRLTVFQRTP
                     PWMVPKEDRPYSAEELARYQRLPWASWRERWRLWKLQHDNTALTPGHPRLGAVQELSE
                     RFLHKHVDDDVLRDSLTPRYPFRCKRVLLGEKYYTALQREHVALVTDPIGRITETGIL
                     TAGGRAVDADAIVLATGFETSSYLSGIDVIGVGGERLHARWGLDPQAYLGVAVSGFPN
                     FFMLYGPNTNQGANSIIYILEAGARFVASAVSRVARRGGFVDVDPRVERRFNDRISAD
                     LEQTIWTRCDSYYRSPSGRIVTQWPRSELDYAKATWRLRSRDWVHGGLE"
     gene            5312..6484
                     /gene="alr"
                     /locus_tag="VGP27_00570"
     CDS             5312..6484
                     /gene="alr"
                     /locus_tag="VGP27_00570"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV7849843.1"
                     /translation="MPTTELTTSVTPSANAEAVVDLASIGHNVRVLREHAGSAQVMAV
                     VKADGYGHGATQVARAALEAGAAEIGVATIEEALALRRDGVTAPVLAWLHPPGTDFAP
                     ALQSGVQVALSSPRQLTELLAAAKLTGVTADVTVKVDTGLSRNGVSPAEFPSMLTGLR
                     HAVADEAIRLRGIMSHLARGDEPDNPVNDLQARRLADMREQARDHGVHFEIAHLCNSP
                     AAMSRPDLAYDMVRPGIAVYGLSPIPERGDMGLRPAMTLKCPVTVVRSVRAGDGVSYG
                     HTWIADRDTTLALLPIGYADGVYRRLSNRMNVLINGRLRPSVGRICMDQFVVDLGPDA
                     GDVREGDEAILFGPGTDGEPTAQDWAEMLDTIHYEVVTSPRGRVVRTYRGAGDGGR"
     gene            6474..7592
                     /locus_tag="VGP27_00575"
     CDS             6474..7592
                     /locus_tag="VGP27_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7849844.1"
                     /translation="MGDNDPKRLGRKANAGLLAGVAGLAAVGALAGVSVARSMTRRTT
                     AGDPYAGEDFELLDADRSSVVTTPDGVDLAVREVGAEHAPLTVVFSHGFCLRMGAFYF
                     QRARLAQEWGPQVRMVFYDHRGHGQSAEASPDTYTVEQLGMDLETVLAVTAPKGPVVL
                     VGHSMGGMTVLSHARQFPRRYPTRVVGVALISSAAEGVARSPLAEILKNPALEAVRFA
                     VRYAPKTVHRTRGAAKSVIAPILRAASYGDEKVSPSVVAFSEKMMHGTPIVTLVEFLH
                     ALEVHDEEAALPTLAKVPTLIACGDRDLITPVEHSEAMAARLPKSELVVVEGAGHLVQ
                     LEQPEIIDDALVRLVERATPSKLVAMTRRLRDRARPHG"
     gene            7585..8049
                     /gene="tsaE"
                     /locus_tag="VGP27_00580"
     CDS             7585..8049
                     /gene="tsaE"
                     /locus_tag="VGP27_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV7849845.1"
                     /translation="MADQAGTAELPTAEDTKALGAQLGGQLHAGDVVVLSGPLGAGKT
                     VLAKGIAEAMDVDGPVTSPTFVLARLHRARQPGAPAMVHVDMYRLLDHAGADLLGELD
                     SLDLDTDLDDAVVVVEWGEGIVERLSERHLDIRLERGADTDVRTAIWQWSRS"
     gene            8046..8684
                     /gene="tsaB"
                     /locus_tag="VGP27_00585"
     CDS             8046..8684
                     /gene="tsaB"
                     /locus_tag="VGP27_00585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV7849846.1"
                     /translation="MTNLVLAIDTATPAVTAGIVRRDGDGIDVLAERVTVDARAHAEQ
                     LTPNVLAALADAGLAVTDLTTVVVGCGPGPFTGLRVGMATAAAYGQALGIPVHGVCSL
                     DAIGVDTAGEVLVVTDARRREVYWARYRDGLRIDGPAVSAPADVPTDVGAVAGSPEHA
                     ALFALLRQPAVYPTASGLVRAVDWTVPPQRLVPLYLRRPDAKPQPVAPVARA"
     gene            8681..9145
                     /gene="rimI"
                     /locus_tag="VGP27_00590"
     CDS             8681..9145
                     /gene="rimI"
                     /locus_tag="VGP27_00590"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV7849847.1"
                     /translation="MTVVYGELGPGDAARCAELEAQLFEGDDPWPAAAFLRELAAQHI
                     HYVAARADDKLVGYAGIARLGRKPPYEYEIHTIGVDPAYQGQGVGRQMLDRMLEFAGD
                     DTVYLEVRTDNTAAIAMYQSAGFVNVGLRKRYYRVSGADAYTMQRPAHGGPA"
     gene            9142..10224
                     /gene="tsaD"
                     /locus_tag="VGP27_00595"
     CDS             9142..10224
                     /gene="tsaD"
                     /locus_tag="VGP27_00595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV7849848.1"
                     /translation="MTSPIPGSSPALRAWAHQSCPRTILAIESSCDETGIGIAELAAD
                     GTVTLLADEVASSVDEHARFGGVVPEIASRAHLEALGPTMRRALDAAAIAKPDVVAAT
                     IGPGLAGALLVGVAAAKAYAVAWQVPFYAVNHLGGHLAADVYDHGPLPECVGLLVSGG
                     HTNLLHVRSLAEPIEELGSTVDDAAGEAYDKIARLLGLGYPGGKVLDDLAREGDRDAV
                     AFPRGMTGPRDDPYAFSFSGLKTAVARYVEKNPTVSQADVAAGFQEAVADVLTAKAVR
                     AATELGVSTLLIAGGVAANSRLRQMAEERCAANGLTLRIPPPRLCTDNGAMIASFAAH
                     LVAAGAPPSPLDVASDPGLPVVKGQL"
     gene            10514..10816
                     /gene="groES"
                     /locus_tag="VGP27_00600"
     CDS             10514..10816
                     /gene="groES"
                     /locus_tag="VGP27_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV7849849.1"
                     /translation="MASVNIKPLEDKILVQANEAETTTASGLVIPDTAKEKPQEGTVV
                     AVGPGRWDEDGEKRIPLDVSEGDTVIYSKYGGTEIKYGGEEYLILSARDVLAIVNK"
     gene            10901..>11240
                     /locus_tag="VGP27_00605"
     CDS             10901..>11240
                     /locus_tag="VGP27_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEV7849850.1"
                     /translation="MAKQIEFNETARRAMEAGIDKLAGAVRVTLGPRGRNVVLAKSFG
                     GPTITNDGVTIAREIELDDPFENLGAQLVKSVATKTNDVAGDGTTTATVLAQALVKGG
                     LRNVAAGANPI"
BASE COUNT         1846 a         3805 c         3853 g         1736 t
ORIGIN      
        1 tcggcgctgt ggttcgccct cgacaccgac ctgcccgtca tcgtcgacgc cgacgggctg
       61 accatcctcg ccgcgcatcc cgacctgatc gtcaaccgca aagcgccgac ggtgctgacc
      121 ccgcatgcgg gcgagttcgc gcggctggct tccggagccg ggctgccgac gacatcggat
      181 caagcggggc caggcgacga ccgcatcgcc gccacccgca ggctctccga ccagttcggc
      241 gccaccgtgc tactgaaggg caacgtcacc gtcatcgccg aacccggggg gccggtgtac
      301 ctcaacccgg caggccagtc ctgggcggcc accgccggat cgggcgatgt gctgtccggc
      361 atcatcggcg cgctgctcgc cacgggactg ccgcccggag aagcggcggc ggccgccgcg
      421 ttcgtgcatg cgcgcgcagc caacctgtcc gccgcggacc ccggcccgca gcccgccccg
      481 acgtcggcgt cgggcatcct cgcccacgtc cgtaccgcaa tcgcgcgtct ccagcagtca
      541 ggaaaggact ctgcatggcc ccgaggcaca cccaaagccc agtgatcgca ccggcataca
      601 ccgggcggat gtcgatggcg cccatcccgt cgctacggat gccggacgaa tcgatgaatc
      661 ccgatgccgc ataccggttc atccacgacg agctgatgct cgacgggagc tcccggctga
      721 acctcgcgac gttcgtgacg acgtggatgg acccgcaggc cgagaagctg atggcggaga
      781 cgttcgacaa gaacatgatc gacaaggacg agtatccggc caccgccgcg atcgaatccc
      841 gctgtgtgtc gatggtggct gacctgttcc acgccgaggg gctgcgcgac gacgacccgt
      901 caagtgcgac aggagtttcc acggtcggct ccagcgaggc cgtcatgctc gccggtctcg
      961 ccttgagctg gcggtggcgc gaacgcgtcg gcaacggaaa agacggctgg cgctcgcgtc
     1021 gacccaacct ggtgatgggc tccaacgtgc aggtggtgtg ggagaagttc tgccggtact
     1081 tcgacgtcga gccgcggtac ctgccgatgg agaagggccg ctacgtcatc acgcccgagc
     1141 aggtactgga gaacgtcgac gaggacacca tcggcgtcgt cgcgatcctg ggcaccacct
     1201 tcaccggcga gctcgaaccg atcggcgaga tctgcgaggc actggacaac ctggcggccg
     1261 gcggcgggct ggacatccct gtgcacgtcg acgcggcgag cggcggcttc gtcgtgccgt
     1321 tccttcatcc cgacatggtc tgggacttcc ggcttccgcg agtggtgtcg atcaatgtca
     1381 gcggccacaa atacggcctg acgtaccccg gcatcgggtt cgtggtgtgg cgcagtcccg
     1441 agcatctgcc ggaggacctg gtcttccggg tgaactacct cggcggcgac atgccgacgt
     1501 tcacgctcaa cttctcccgg cccggcaatc aggtggtcgg ccagtactac aacttcctgc
     1561 ggctcggacg ggagggctac tcgcaggtga tggagtgcct gtcgcagacc gcacggtggc
     1621 tgggtgacca attgcgcgaa agcgagcact tcgagctgat ctccgacggc tctgcgatcc
     1681 cggtggtcag cttccggctg aagggcgatc cggcctatac ggagttcgac gtctcagagg
     1741 cgctgcggtc attcggctgg caggtgcccg cctacacgat gccggagaac gccaccgaca
     1801 tcgccgtgct gcgcgtcgtc gtgcgcgaag gtttctcgat gaatttggcc cgagcactgc
     1861 gcgacgatgc cgtcaccgcg ctgaaacacc tcgacgagct caagccggac ggtcatttca
     1921 acgagctgca gcccttcgcg cactgacggt gaatccgtga aagaccctga cgcggcgttc
     1981 gacgtcgaag ccttgcggga tagatacgcc cgcgagcgcg gccgacgact gcggcccgac
     2041 ggaatcgccc agtacgtcga gatcgctggg cccttcgccg gattcgccga agatccttgg
     2101 agcgaaccgc atttcactcg cgcgccgttg caggatgagg tcgacgtcgc gatcgtcggc
     2161 gccgggttcg gggggctgct gatcggggcg cggctgcgcg agctcggggt gcagagcatc
     2221 cggatcatcg acaaggccgc cgacgtgggc ggcacctggt actggaaccg gtaccccggc
     2281 atcgcttgtg acgtcgagtc ttacgtctac atgccgctgc tggaggagct cggttaccta
     2341 ccgaccgaga aatacgccaa gggcgacgag atcctcgctc actgcaggcg catcgccgaa
     2401 cactacgacc tctaccgcga tgcctgcctg caaacggagg tcaccgaaat ccgctgggac
     2461 ggccggcttt cccgctggat cgtcgccacc aaccacggtg acgcgattca ggcgcggttc
     2521 gtcgcgatgg ccaacggtta cctgcagaag ccgaaacttc ccggcatcga agggatcgcg
     2581 gactttcgcg gccacgcatt ccataccagc cgctgggact acggctacac tggccgccat
     2641 ctggccaagt tggccgacaa gcgggtagga atcatcggca ccggtgcgac cgcgattcaa
     2701 tgcgtaccgc acctcggcag gtcggtcgag cacctgttcg tctttcaacg cacaccgtcg
     2761 acggtggatg tgcgcggcaa ccagcagacc gacccggaat ggtcggcgga gctgaagaac
     2821 ggttggcagc gccaccggat cgagaacttc cagctgctca ccgcgggcgg cgaggccgat
     2881 gaggacctgg tcggcgacgc ctggacgagc atcgtcaaaa aactgttcgt gatgcgacag
     2941 cgcagcgtgg ccggcatgtc ggacgaggac cgtttgcgcg cagtcgaaat ggccgacttc
     3001 accaaaatgg aggagatccg cgcgcgcgtc gactcggtcg tgtccgaccg tgccacggcg
     3061 gagggcctca agccctggta cggctacttc tgcaagcggc cgtgcttcca cgacgagtac
     3121 ctgcagacgt tcaaccgaga caacgtcacc ctggtcgaca cccggggcct cggcgtccag
     3181 cgcatcacgc ccggcggtgt cgtggtcgac ggcaccgaac acgtcgtcga ctgcctgatc
     3241 ttcgcgaccg gattcgaggt cggcaccgac tactcgcggc gcaccgggtt cgaactcgtc
     3301 ggacgcgacg gactggcgct gaccgacaaa tggcgagacg gcgtccgcac attgcacggc
     3361 ctcagcgttc gcggcttccc gaactgtttc gtgctcagca tcgcccagtc gggtttcacc
     3421 gtgaacttcc cctatctcct cgatgtccag gccacccata ccgcctggat gatcgcctgg
     3481 gcactgcgca atgcggtcga cagcctcgag gcctccgccg aagccgagtc ggcatgggtc
     3541 ggcaccgtcg tcgagcggtc gtcggcgagc ctggcaaggg ccaaggcgtg caccccgggc
     3601 tactacaacc gggagggcca ggcgaacgcg aagacgcgtc agggcagctt ctttttcggc
     3661 agcccgaccg aatatgccga catcctcgcc gcatcgcggg cgaacgggtc accggagggg
     3721 ttcgagtttt cgtgacgcgc cgctatctcg tcgggcggct gtcggcgctc acgcgtcatc
     3781 gccggtcgcc gcgagtggtc atcatcgggg ccggcttcgg cgggctcgcg gcagcggtcg
     3841 cgttgcgccg caagggcatc gacgacctga cgatcatcga acgcgccgac ggtgtcggcg
     3901 ggacctggcg gcgaaacgtc tacccgggcg cggcgtgcga catccagagc cacctgtatt
     3961 cgctgtcgtt cgcgccgaac cgggcgtgga cccgtacgta tgcgtatcaa ccggagatcc
     4021 tcgcgtacct ggaatcggtc gccgacgagt tcgacttgcg gaggcatctg gtgctgaaca
     4081 ccgaggtgcg caccatgcgg tgggacgccg acgcgtcgca ctgggacgtg gagttggccg
     4141 gcggccacac cgtcgtcgca gacgtcgtcg tcagcgcagt cgggctgttc ggttccgtcc
     4201 ggtaccccga tatcgacggg gtcgccggct tctccggcac cctgatgcac accgcgcaat
     4261 gggatccgac cgtcgacctg accggcaagc gggtggcggt gatcggaacg ggtgccagcg
     4321 gggtgcaggt cgtgcccgaa ctcgcagaga tcgcaggccg attgaccgtc ttccagcgca
     4381 caccgccgtg gatggtgccg aaggaggacc ggccctacag tgccgaggag ttggcgcggt
     4441 accaacggct gccctgggct tcctggcgcg aacggtggcg gctgtggaaa ctgcagcacg
     4501 acaacaccgc attgacgccg ggccatcctc ggctgggggc cgttcaggaa ctgtccgagc
     4561 ggttcctgca caagcacgtc gacgacgacg tgctgcgcga ttcgctgacg ccgcgctacc
     4621 cgttccggtg caagcgagtt ctgctcggcg agaagtacta caccgcattg cagcgcgagc
     4681 acgtcgctct cgtcaccgac ccgatcggcc gcatcaccga gaccgggatc ctcaccgcag
     4741 gcggaagggc cgtcgacgcc gacgccatcg tgctggcgac gggcttcgaa accagcagtt
     4801 acctgtcggg aatcgacgtg atcggcgtcg gcggcgaacg cctgcatgcg cgttggggtc
     4861 tcgacccgca ggcctatctg ggtgtagccg tcagcggctt cccgaacttc ttcatgctgt
     4921 acggcccgaa caccaaccag ggggccaact cgatcatcta catcctggag gcaggcgcgc
     4981 ggttcgtcgc cagcgcggtc agtcgcgttg cgcgccgcgg cgggttcgtc gacgtcgacc
     5041 cccgcgtcga gcgccggttc aacgatcgga tctccgcgga tctcgagcag acgatctgga
     5101 cacgttgcga cagttactac cgctcgccct ccggccggat cgtcacgcaa tggccccgtt
     5161 cggagctcga ctacgcgaag gcgacatggc ggctgcggtc acgcgactgg gtgcacggcg
     5221 ggctcgagta ggccattcgt tagctgcctg cgatacacgc tggtgggagc gctatcgccg
     5281 gtctgggaca atcgggggtg gtgacatgac catgccgacg accgaactga cgacttctgt
     5341 gacgcccagc gccaacgccg aggcggtggt cgacctcgcc tcgatcgggc acaacgtccg
     5401 cgttctgcgt gagcacgccg gttcggcgca ggtgatggcg gtcgtcaagg ccgacggcta
     5461 cggccatggg gccacccagg tcgcacgcgc ggcgctggag gcgggcgccg ccgagatcgg
     5521 cgtcgccacc atcgaggagg cactcgcact gcgccgcgac ggcgtcaccg cgccggtact
     5581 cgcatggctg catccgcccg gcaccgactt cgcgccggcg ctgcagtccg gcgtccaggt
     5641 cgcactgtcg tcgccgcggc agctgaccga gttgctggcc gcggccaagc tcaccggagt
     5701 cacggccgac gtcacggtga aggtcgacac cgggttgagc cgcaacggcg taagtcccgc
     5761 cgagtttccg tcgatgctga cggggctgcg tcatgcggtg gccgacgagg cgatccggtt
     5821 gcgcggcatc atgtcgcacc tggcccgcgg cgacgagccg gacaaccccg tcaacgacct
     5881 gcaggcgcgg cggctggccg acatgcgcga gcaggcccgc gaccacggag tgcacttcga
     5941 gatcgcgcac ttgtgcaact cgcccgcggc gatgtcgcgt cccgacctcg cctacgacat
     6001 ggtccggcct ggcatcgcgg tgtacggcct gagcccgatc ccggaacgcg gtgatatggg
     6061 gctgcggccc gcgatgacac tgaaatgccc tgtgacagtg gtcaggtcgg tgcgcgccgg
     6121 cgacggcgtg tcctacgggc acacctggat cgccgatcgc gacaccaccc ttgcgctgct
     6181 gccgatcgga tacgccgacg gcgtgtaccg caggttgagc aaccggatga acgtcttgat
     6241 caacggcagg ctgcggccca gcgtcggccg catctgcatg gaccagttcg tcgtcgacct
     6301 cggccccgac gcaggcgatg tccgtgaggg cgacgaggcg atcctgttcg ggccgggcac
     6361 cgacggtgaa ccgaccgccc aggactgggc cgagatgctg gacaccattc actacgaggt
     6421 tgtcaccagt ccgcggggtc gggtcgtcag gacatatcgc ggagcaggtg acggtgggcg
     6481 ataacgaccc gaaacggctg gggcgcaagg cgaacgccgg cctgttggcg ggtgtcgctg
     6541 ggctggccgc cgtcggcgcc ctcgccgggg tgtccgtcgc gcggtcgatg acacggcgca
     6601 ccaccgccgg ggatccctat gcgggtgagg atttcgagct gctcgacgcc gaccgcagct
     6661 ccgtggtgac cacgccagac ggggtggacc tcgcggtgcg ggaagtcggt gccgagcatg
     6721 cgcctctgac tgtcgtgttc tcccacgggt tctgtctgcg catgggcgcc ttctactttc
     6781 agcgagcgcg gctggcacag gagtggggcc cgcaggtgcg gatggtgttc tacgaccatc
     6841 gcgggcacgg ccagtcggcc gaggcgtcgc cggacaccta taccgtcgag caactgggca
     6901 tggacctgga aaccgtgctc gccgtgacgg cacccaaggg acccgtggtg ctcgtcgggc
     6961 actcgatggg cggcatgacg gtgctctcgc acgcccggca gtttcctcgt cgctacccca
     7021 cccgcgtcgt cggcgttgcg ctgatctcct cagcggcaga aggtgttgcg cggtcaccgc
     7081 tggcagagat cctgaagaac cccgcgctgg aggcggtgcg gttcgcggtc cggtacgcgc
     7141 cgaagaccgt gcaccgcacc aggggcgcgg ccaagtccgt catcgcaccg atcctgcggg
     7201 cggcgtccta cggcgacgag aaggtcagcc ccagcgtggt ggcgttctcc gagaagatga
     7261 tgcacggcac gccgatcgtc acactcgtcg agttcctgca cgccctggag gtgcacgacg
     7321 aggaagccgc gctgcccaca ctggccaagg tgccgacgct gatcgcctgc ggcgaccgcg
     7381 atctgatcac gccggtggag cactccgagg cgatggcggc caggctgccg aagtcggaac
     7441 tggtggtcgt cgagggcgcg ggtcacctgg tgcaactcga gcagcccgag atcatcgacg
     7501 acgcgctggt ccggctggtc gagcgggcca ccccgtccaa actggtggcg atgactcgcc
     7561 gcctgcgcga tcgggctcga ccccatggct gatcaggccg ggacagcaga gctgccgacc
     7621 gccgaggaca ccaaggcgct gggtgcccag ctcggcggcc agctgcacgc cggtgacgtg
     7681 gtggtgttgt ccggaccgct gggtgcggga aagacggtgc tggccaaggg gatcgccgag
     7741 gcgatggatg tggacggccc cgtgacgtcg ccgaccttcg tgctggcccg cctgcatcgg
     7801 gcgcgacagc ccggggcgcc cgccatggtg catgtggaca tgtaccgact gctggaccac
     7861 gccggcgccg accttctcgg cgaactcgac tcgctcgacc tggacaccga cctcgacgac
     7921 gcggtcgtcg tcgtcgaatg gggcgagggc atcgtcgaac ggctctcgga gcggcacctc
     7981 gacatccggc tcgagcgcgg cgcggatacc gacgtgcgga cggcgatctg gcagtggagc
     8041 cgatcatgac gaatctcgtc ctcgcgatcg acaccgcgac gcccgctgtc accgccggaa
     8101 tcgttcggcg cgacggcgac ggcatcgacg tgctcgccga acgggtgacc gtcgacgctc
     8161 gcgcgcatgc cgaacagctg accccgaatg tgcttgccgc gctcgccgac gcagggctgg
     8221 ccgtgaccga tctgaccacc gtcgtggtgg ggtgcggccc agggccgttc accgggcttc
     8281 gggtcggcat ggccaccgcc gccgcctacg ggcaggcgct cggcattccc gtccacggcg
     8341 tatgcagcct ggacgcgatc ggcgtcgaca cggcggggga ggtgctcgtc gtcacggacg
     8401 cccgtcgccg cgaggtgtac tgggcgcgct accgcgacgg cctgcgcatc gacggacctg
     8461 ccgtcagcgc acccgccgac gtaccgaccg acgtcggcgc cgttgccggt tcgcccgagc
     8521 acgcggcgct gttcgccctg ctgcggcagc ccgcggtcta cccgacggca tcgggtctgg
     8581 tgcgcgccgt cgactggacg gtgccgccgc agcggctcgt tccgctctac ctgcgcagac
     8641 ccgatgccaa gccgcagccc gttgcaccgg tggcgcgcgc atgaccgtcg tctacggcga
     8701 gttggggccg ggcgacgcgg cgcgatgcgc ggagctggag gctcagctgt tcgaaggcga
     8761 cgacccgtgg ccggcggcgg ccttcctccg cgagcttgcc gcgcagcaca tccactatgt
     8821 cgccgcccgc gccgacgaca agctcgtcgg atacgcgggt atcgcgcgcc tcggccgaaa
     8881 gcctccctac gagtacgaga ttcacaccat cggtgtggat cccgcatacc agggacaggg
     8941 tgtcggcaga cagatgcttg accgcatgct ggagttcgcc ggcgacgaca ccgtctatct
     9001 cgaggtacgg accgacaaca cggcggcgat cgcgatgtac cagagcgccg ggttcgtcaa
     9061 cgtcgggctg cgcaagcgct actaccgggt gagcggcgcc gacgcctaca ccatgcaacg
     9121 tcccgcccac ggaggtccgg catgacttca cccatccccg ggtcgtcccc tgcccttcgg
     9181 gcgtgggccc accagtcctg ccccaggacc atcctcgcta tcgaaagctc ctgtgacgaa
     9241 accggaatcg gcatcgctga gctggcagcc gacggcacgg tgacactgct ggccgacgag
     9301 gtggcctcga gcgtcgacga acacgcacgg ttcggcggtg tggtgcccga gatcgcgtcc
     9361 cgcgcgcatc tggaggcgct ggggccgaca atgcgccgtg cgctcgacgc ggcggccatc
     9421 gccaagccgg acgtcgtcgc cgccacgatc gggcccggtc tggccggcgc cctgctggtg
     9481 ggcgtggccg cggccaaggc ttacgcggtc gcgtggcagg tgccgttcta cgcggtcaat
     9541 catctcggcg gccacctcgc cgccgacgtg tacgaccacg gcccgctgcc cgaatgtgtc
     9601 ggcctgctgg tttccggcgg ccacaccaac ctgctgcacg tgcggtcgct ggcggaaccg
     9661 atcgaagaac tcggcagcac cgtcgacgac gccgcggggg aggcctacga caagatcgca
     9721 cggctgctcg gcctcggcta ccccggcggc aaggtgctcg acgacctggc gcgcgagggc
     9781 gaccgcgacg cggtcgcgtt cccacgcggc atgaccgggc cgcgtgatga tccgtacgcc
     9841 ttcagcttct cgggtctcaa gaccgcggtc gcgcggtatg tcgagaagaa ccccaccgtg
     9901 tcgcaagccg acgtggcagc cggcttccag gaggcggtgg ccgatgtgct caccgcgaag
     9961 gcggtgcggg ccgccaccga gctcggggtg tcgacactgc tgatcgccgg tggcgtcgcc
    10021 gcgaactcgc ggcttcgtca gatggccgag gagcgctgtg cggcaaacgg tttgacgctc
    10081 cggatcccgc caccgcggct gtgcaccgac aacggagcga tgatcgcgtc gttcgcggcg
    10141 cacctggtcg cggcgggcgc gcccccgtct ccgctggacg tcgccagcga ccccggtctg
    10201 ccggtggtga aaggtcagct gtgagaactg gcggccgccg atgtgttcga cccagcgcgc
    10261 cgacctcttc aaccgctggt catcgcgtat ttcggcgatg ttcgcgtccg gcacgggaga
    10321 taggcagggg gcgaccttga gtgctagcac tctcatgtat agagtgctag gtggcaggcg
    10381 gccacgccct cgaccggcac ccgcgacgac ggcgcgaggg cacggacgca tgccgaacac
    10441 ctgttaatgc tgtcaagtca gtagatccgg ggatgcgccc cgggtcgacc ccgatctaac
    10501 ggagggctcc atcgtggcga gcgtgaacat caagccactc gaggacaaga tcctcgtaca
    10561 ggccaacgag gccgagacca cgaccgcgtc cggtctggtc attcccgaca ccgccaagga
    10621 gaagccacag gaaggcaccg tcgtcgcagt cggccccggc cggtgggacg aggacggcga
    10681 gaagcggatt ccgctggacg tgtcggaggg cgacacggtc atctacagca agtacggcgg
    10741 caccgagatc aagtacggcg gcgaggagta cctgatcctc tccgcccgcg acgtgctggc
    10801 catcgtcaac aagtaacgac gaaacgtgac cgccccggcg atccccgcgt aaagcggcgg
    10861 attcccgggg cgtcatgcat agcaaggacg gactcgtttt atggctaaac agatagaatt
    10921 caacgaaacc gcgcgtcgcg caatggaagc cggcatcgac aagctcgccg gtgcggtccg
    10981 cgtgacgctc ggcccgcgcg gcaggaacgt ggtgctcgcc aagtcgttcg gcggcccgac
    11041 gatcaccaac gacggtgtca ccattgcccg cgagatcgag ttggacgacc cgttcgagaa
    11101 cctgggcgct cagctggtga agtcggtcgc gaccaagacc aacgacgtcg ccggtgatgg
    11161 cacgacgacg gcgaccgtgc tggcacaggc gttggtcaag ggggggctgc gcaacgtcgc
    11221 cgccggtgcc aacccgatcg
//
LOCUS       DASYLX010000020         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3512008,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000020 DASYLX010000000
VERSION     DASYLX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512008"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGP27_00610"
     CDS             complement(<1..488)
                     /locus_tag="VGP27_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7849851.1"
                     /translation="MEGQRSLESFGYTQELHRSVATVDLLIYGLIFMVPIAPWAIFGT
                     VYNSAAGMVPLVYLIGLVAMVFTAMAYAQMARSFPLAGSVFSYVGRGIHPGAGFFAGW
                     AILLDYLLVPTLLYVFAAESMIGLFPGTPRWMWAIVFVVVNTVINLLGVDSLKMANRV
                     FLA"
     gene            complement(514..1425)
                     /locus_tag="VGP27_00615"
     CDS             complement(514..1425)
                     /locus_tag="VGP27_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEV7849852.1"
                     /translation="MPRSTQMVPFRSHQTWVQITAPEDPRHGAQPLFVLHGGPGMAHN
                     YVRNIAELADETGRTVIHYDQIGCGNSTHLPDAPADFWTPDLFVEEFHTVREALGIDE
                     YHLLGQSWGGMLSAEIAVLQPSGLTSVAICNSPASMELWMAGAAELRAALPPDVQDAL
                     TRHEEAGTVTDPEYLAATDEFYRRHLCRVVPTPQDLVDTIEQMEAEPTVYHTMNGPNE
                     FHVLGTLRGWSIVDRLPQITAPTLVIAGEFDEATPQTWQPYVDQIAGADSHVFPATSH
                     CSHLEKPEEFRAVVAAFLARHDATARV"
     gene            1512..2321
                     /locus_tag="VGP27_00620"
     CDS             1512..2321
                     /locus_tag="VGP27_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7849853.1"
                     /translation="MAHNSAPGGLSHQQRDTPTRVDEITDRLITAIAIGEYLPGARLP
                     VERDLAASLRAGRMTVRAALARLLDRGLIETRRGRGGGSYVREQWPDSSTDAVGRTLR
                     ARLGELRDRCDAICRLQGTVSRAAAESRTEHDISAIQARFEAYRTAKSGLDAQQADSR
                     LHLAIMDAAHNDVLKQVLLDVKATVSIGAPAHLWGEPSTMRTMELRALDEHEQLVGAI
                     ADGRADDADDLARHHGLIDFELITTAMERAGVFTDAPSSAARPTAWRRVVS"
     gene            complement(2314..>2708)
                     /locus_tag="VGP27_00625"
     CDS             complement(2314..>2708)
                     /locus_tag="VGP27_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV7849854.1"
                     /translation="LNQFYERSARPSTQDLIFLSTHEPCPLCLSAITWCGFDNFYYFF
                     TYEDSRDAFGIPHDLKILKEVFGVEAGAYRRKNAFWTAYSIGALVETEAEPLRTRLRE
                     QDKRIRAVYAALSEQYQSSKADNTIPFN"
BASE COUNT          509 a          816 c          926 g          457 t
ORIGIN      
        1 gccaagaaca cgcggttcgc catcttgagc gagtcgacgc cgagcagatt tatcacggtg
       61 ttgacgacga cgaacacgat cgcccacatc caccggggcg ttccgggaaa caggccgatc
      121 atcgactctg ctgcgaagac gtacagcagt gtcggcacca gcaagtagtc gagcagaatt
      181 gcccagcccg cgaaaaatcc ggcgcccgga tggattccgc ggccgacata ggagaacacc
      241 gagcctgcga gcgggaacga tcgggccatc tgcgcgtagg ccatcgcggt gaacaccatc
      301 gccaccaggc cgatgaggta gaccaggggc accatgccgg cggcgctgtt gtagacggtg
      361 ccgaaaatag cccacggcgc gatcggcacc atgaatatca ggccgtagat gagcaggtcg
      421 acggtcgcga ccgagcggtg caattcctgt gtgtagccga aggattcgag gctgcgttga
      481 ccttccatgg ctgtcctgtc gggtcgaggt gggctagacg cgggctgtcg cgtcgtgacg
      541 ggcaaggaac gcggcgacga cggcgcgaaa ctcctctggc ttctccaggt gcgagcagtg
      601 actggtggcg gggaagacgt ggctatccgc acccgcgatc tgatcgacat acggctgcca
      661 ggtctgcgga gtcgcctcgt cgaactcgcc ggcgatgacc agtgtgggcg cggtgatctg
      721 aggcaaccgg tcgacgatgc tccacccgcg cagtgtgccg agtacgtgaa actcgttggg
      781 gccgttcatg gtgtggtaca ccgtcggctc ggcttccatc tgctcgatgg tgtcgacgag
      841 gtcttgcggc gtcggcacga cccggcacag atgtcgccgg tagaactcgt cggtcgccgc
      901 cagatactcc ggatcggtca cggtgcccgc ttcctcgtga cgggtcagcg cgtcctgtac
      961 gtcggggggc aaggcagccc gcaactcggc ggcgccggcc atccagagtt ccatcgatgc
     1021 cggcgagttg cagatcgcca ccgacgtcag ccctgatggt tgcaggacgg cgatctcggc
     1081 gctcagcatg ccgccccagg actggccgag caggtggtac tcgtcgatgc ccagagcttc
     1141 gcgcaccgta tggaactcct cgacgaacag gtcgggcgtc cagaagtccg cgggtgcgtc
     1201 gggcaggtgt gtgctgttgc cacatccgat ctggtcgtag tggatgacgg tgcggccggt
     1261 ttcgtcggca agttcggcga tattgcggac atagttgtgc gccatgccgg gtccgccgtg
     1321 caggacgaac agcggctgtg cgccgtgccg ggggtcctcc ggtgcggtga tctggaccca
     1381 ggtctggtgg ctgcggaagg ggaccatctg ggtgctacga ggcatgaagt tcagcatgga
     1441 ccggtaaagg tctcatgaca agaccattga tcgggattta acattcaaga tgacaacgaa
     1501 ggaatgaccg catggcgcac aacagcgcac cgggaggctt gtcacaccag caacgtgaca
     1561 cgcccactcg cgtcgacgag atcaccgatc ggctgatcac cgcgatagcg atcggggagt
     1621 acctgcccgg cgcgcggttg ccggtcgaac gcgatctcgc tgcgtcgctg cgggcgggtc
     1681 gcatgacggt gcgcgctgcg ctggcccgcc ttctcgatcg gggactgatc gaaacgcgac
     1741 ggggacgcgg cggcgggtcg tacgtccggg agcaatggcc agactcgtcc accgatgcgg
     1801 tcggccgcac gctgcgggcg cgactgggcg aactacgaga ccgctgcgac gcgatctgcc
     1861 gactgcaggg caccgtcagc cgggccgccg cggagtcgcg tactgaacac gacatatcgg
     1921 cgattcaagc caggtttgag gcgtaccgga cagcaaagag cggcctggac gcacagcagg
     1981 ccgacagccg gctgcacctg gcgatcatgg atgcggccca caacgacgtt ctcaagcagg
     2041 tgctgctcga cgtgaaagca acggtcagta tcggtgcgcc ggcacacctt tggggcgagc
     2101 cgtccaccat gcgcaccatg gagctccgcg ctcttgacga acatgaacag ctggtgggtg
     2161 ccatcgccga cggccgcgcc gacgatgccg acgatcttgc gcgtcatcac ggcctcatcg
     2221 acttcgaact catcacgaca gcgatggagc gcgccggggt gttcaccgac gcaccgagtt
     2281 ccgccgcgcg tccgactgct tggcgacgag tggtcagttg aacggaatgg tgttgtcggc
     2341 cttggaactc tgatactgct cggacagggc cgcgtagaca gcacggatcc gcttgtcctg
     2401 ctcgcgcagc cgggtgcgga gcggctcggc ttcggtctcg accagtgcac cgatcgagta
     2461 ggcagtccag aacgcgttct tgcgccggta cgcaccggcc tcgacgccga agacctcctt
     2521 gaggatcttc aggtcgtgtg gaatgccgaa cgcgtcgcgg gaatcctcgt aggtgaagaa
     2581 gtagtagaag ttgtcgaatc cgcaccaggt gatagccgac aggcacagtg ggcagggttc
     2641 gtgggtggac aggaaaatca agtcctgcgt cgacggccga gcggaccgct catagaactg
     2701 gttcagtg
//
LOCUS       DASYLX010000021         5262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_879295,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000021 DASYLX010000000
VERSION     DASYLX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879295"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGP27_00630"
     CDS             complement(<1..438)
                     /locus_tag="VGP27_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEV7849855.1"
                     /translation="MAGRIGIDDVAPVVSAGRYPAKAVVGEVVPVTATVWREGHDAVA
                     ATLVVRYHGTDYPQLTDGPAQNPAPQPVPIETVVTPAPKIKPQAIPMATGRTPDVFHG
                     VFSPDRVGLWTFRVDGWGDPIATWRHNVTAKLDAGQSETELSND"
     gene            608..3226
                     /gene="glgP"
                     /locus_tag="VGP27_00635"
     CDS             608..3226
                     /gene="glgP"
                     /locus_tag="VGP27_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV7849856.1"
                     /translation="MKALRRFTVRAHLPERLVALERLSTNLRWSWDKPTQDLFAAIDP
                     ALWTQVGCDPVALLGQVSPKRLDELAADDLFADRLDKVAAHLDDYLSRPLWYQERAAE
                     GTELPNGIAYFSMEFGVAEVLPNYSGGLGILAGDHLKSASDLGLPLIAVGLYYRSGYF
                     RQSLTADGWQRENYPSLDPQGLPLRLLTDHQGDPVLVELAMPDAAQLRARVWVAQVGR
                     IPLLLLDSDIPENEHELRGVTDRLYGGDQEHRIKQEILAGIGGVRAIRAFTEIENLPT
                     PEVFHMNEGHAGFLGVERIRELIGSGLDFDTALTVVRSSTVFTTHTPVAAGIDRFPVE
                     MVRRYFGGEGSPSGATSRLLPGVPLDRVIAFGAEDDPSKFNMAHMGLRLAQRANGVSL
                     LHGRVSREMFNELWPGFDPSEVPIGSITNGVHAPTWAAPQWLELGRELSGGDLPGAPA
                     VWEPALWERLQQVDPGHLWWIRSQLRELLVNDVRARLRRSWLERGASDAELGWIATAF
                     DANVLTIGFARRVPTYKRLTLMLRDPDRLEKLLLDEKRPIQLIVAGKSHPADEGGKAL
                     IQQVVRFADRPEVRHRIAFLPDYDMSMARQLYYGCDVWLNNPLRPLEACGTSGMKSAL
                     NGGLNLSIRDGWWDEWYDGENGWEIPTADGLADESRRDDLEAAALYSLVERSVAPKFY
                     DRDDRGVPMRWVEMVRHTLEVLGPKVLASRMVRDYTEKYYAPAAQSLRRTVVPLEEGG
                     VPFGAAWDLAAYRQRAHEAWPKIQITDVDSTGLPDTPLLGSELTLSATVQLAGLGPDE
                     VSVQAVLGRVDPADALVNPKTVPMLHTGTAEGGNEIFSTTTPLPVAGPVGYTVRVLPH
                     HPLLASDSELGLVTLA"
     gene            complement(3278..4726)
                     /locus_tag="VGP27_00640"
     CDS             complement(3278..4726)
                     /locus_tag="VGP27_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV7849857.1"
                     /translation="MSRRPVTGALLAVCAALFVASCSTTVKGTAVSVFDDPFKVAGMP
                     AVDGPTGLRPDAKKASRDVENGDGGEVDELGADSVSDIEDFWETTYAETFGTSFSPVE
                     ALISWDAGGYESQEFCEEDTYGLVNAGYCFLDNTIGWDRGELLPALRDNFGDIAVTNV
                     LAHEYGHAIAHQAKINKKNIPTLVAEQQADCFAGAYMRWVAEGNSARFTLSTADGLNR
                     LLAALISFRDPLLSEDQYAPGGNEHGSAFERVTAFQFGFTDGASSCAAIDAKEIAQRR
                     GDLPVELQQDQTGEWPVSEESVKAIVEALETLFSPADPPKLSLDAGSANDCHDARPSP
                     PVSYCPASNTISVDMPKLASMGAPSGQDENGGAVTGDNTAYSVLVSRYMLALQHQRAG
                     VRLDNAEAALRTACLTGVATTKMTAAVTTSDGNTVALTAGDLDEAVSGLLTNGLAASD
                     VNGDTVPSGFSRMDAFRTGVLGDEDRCFNRFP"
     gene            complement(4732..>5262)
                     /locus_tag="VGP27_00645"
     CDS             complement(4732..>5262)
                     /locus_tag="VGP27_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEV7849858.1"
                     /translation="RTLGLFSSMRAARAAAEIMRERLSTPVLCQGDDTTSALVKQFAD
                     DPATCLFGTLSLWQGVDVPGPSLSLVLIDRIPFPRPDDPLLSARQRAVAARGGNGFMA
                     VAASHAALLLAQGAGRLLRRSEDRGVVAVLDSRMATARYAGYLLASLPPFWATTDSVR
                     VRQALERLHAANAALS"
BASE COUNT          828 a         1767 c         1777 g          890 t
ORIGIN      
        1 gtcgttggac agttcggtct cgctctggcc cgcgtcgagt ttggcggtca cattgtgtcg
       61 ccacgtcgcg atcgggtcgc cccagccgtc cacccgaaac gtccacaacc cgacacggtc
      121 aggactgaac acgccgtgga aaacgtccgg tgtgcgaccg gtcgccatcg gaatcgcctg
      181 cggcttgatc ttcggcgccg gggtgaccac ggtctcgatc ggcaccggct gcggggccgg
      241 attctgcgcc ggcccgtccg tgagctgcgg atagtccgtg ccgtggtaac gcaccaccag
      301 ggtggccgcc acagcgtcgt ggccttcacg ccacactgtg gccgtcaccg gcaccacctc
      361 gccgacgacg gccttggcgg gatagcgtcc tgccgacacc acaggtgcca cgtcatcgat
      421 accgatacga ccggccacct accactcctt caaggcgcgg cccggtgcag ggccgctgtc
      481 gtctggattc gtttacagcc tcgtcgcgcc ctatacccac cgtagtgtgc ggcccaacca
      541 agtgctggtg aacgatcgcc ccggcacgag actgcattca cgtcgatcat cagtaaggtt
      601 gggtcgcgtg aaagccctcc gtcggttcac cgtccgcgcg catctcccgg agcgactggt
      661 cgcgctcgag cggctttcga ccaacctgcg ctggtcgtgg gacaaaccca cacaagacct
      721 cttcgcggca atcgacccgg cgctatggac gcaggtcggc tgcgatccgg tggcactgct
      781 cggtcaggtc agcccgaagc ggctggacga actcgctgcc gacgacttgt tcgctgatcg
      841 gctcgacaag gtcgccgccc acctcgacga ctatctgagc cgtccgctgt ggtatcagga
      901 acgcgccgcc gagggcaccg agctgccgaa cggcatcgca tacttctcga tggagttcgg
      961 ggtcgccgag gtgctgccga actactcggg tggcttggga atcctcgccg gtgaccactt
     1021 gaagtcggca tcggatctgg ggctgccgct gatcgcggtg gggctgtact accgctcagg
     1081 ctacttccgt cagtcgctga ccgccgacgg ctggcagcgg gagaactacc cgtcgctgga
     1141 cccgcagggg ctgccgttgc ggttgctcac cgatcaccaa ggcgacccgg ttctggtgga
     1201 gttggccatg cccgacgccg cccagttgcg agcccgggtg tgggtcgcgc aggtcggtcg
     1261 aatcccgttg ctgctgctcg attccgacat cccggaaaac gagcacgaac tgcgcggcgt
     1321 caccgaccgg ctttacggcg gcgaccagga gcaccgcatc aagcaggaaa tcctggcagg
     1381 catcggcggg gtgcgtgcca tccgggcgtt caccgagatc gaaaatctgc cgacgcccga
     1441 ggttttccac atgaacgagg gccacgccgg tttcctcggc gtcgagcgga ttcgcgagct
     1501 catcggctcc ggtctcgatt tcgacaccgc gcttaccgtg gtgcggtcgt ccaccgtgtt
     1561 caccacccac actcccgtcg ccgccggcat cgaccggttc ccggtggaga tggtcaggcg
     1621 ctacttcggc ggggagggca gcccgtcggg cgccacctcg cgcctgctgc ccggtgtccc
     1681 gctggaccgc gtcatcgcgt tcggagccga ggacgacccg tcgaagttca acatggctca
     1741 catgggactg cggctcgcgc agcgcgccaa cggtgtgtcg cttctgcatg gtcgggtcag
     1801 cagggagatg ttcaacgagc tgtggccggg cttcgacccg tcggaggtgc cgatcggctc
     1861 gatcaccaac ggtgtgcacg cgccgacctg ggcggcgccg cagtggctcg agctgggccg
     1921 tgagctcagt ggcggcgacc ttccgggtgc gcccgcggtg tgggagcctg cgctctggga
     1981 acggctgcag caggtggacc ccggacatct gtggtggatc cgttcgcagc tccgcgagct
     2041 gttggtcaac gacgtccggg cgcgactgcg ccggtcctgg ctcgagcgtg gtgcatccga
     2101 cgccgaactc ggctggattg caacggcttt cgatgcgaat gtgctgacga tcgggttcgc
     2161 ccggcgagtg ccgacctaca agcggctgac gctgatgctg cgtgatccgg accggctgga
     2221 gaagttgctg ctcgacgaga agcggccgat tcagctgatc gtggcgggca agtcgcatcc
     2281 ggccgacgaa ggcggaaagg cgctgatcca gcaggtggtg cggttcgccg accggcccga
     2341 agtgcggcac cgcatcgcgt ttctgcccga ctacgacatg tcgatggcgc gccagttgta
     2401 ctacggctgc gacgtctggt tgaacaaccc gctgcgcccg ctggaagcat gcggcacgtc
     2461 ggggatgaag agcgcgctca acggcgggtt gaacctgtcc atccgagacg gctggtggga
     2521 cgagtggtac gacggcgaaa acggttggga gattccgact gccgacggac tggccgacga
     2581 aagccggcgc gacgacctgg aggccgcggc gctctacagc ctggtcgagc ggtcggtcgc
     2641 gccgaagttc tacgaccgtg acgaccgcgg tgtacccatg cgctgggtcg agatggtccg
     2701 ccacacgctc gaggtgcttg ggcccaaggt gctggcatcg cggatggtgc gcgactacac
     2761 cgagaagtac tacgctcccg cggcgcagtc actgcgccgg accgtcgtgc cccttgagga
     2821 gggcggcgtg cctttcggtg ccgcttggga tctggccgcc taccgtcagc gggcccacga
     2881 ggcgtggccg aagattcaga tcaccgacgt cgacagcacc gggctgccgg acactccgct
     2941 gctgggttcc gagctcacgc tctcggcgac ggtgcagctc gcgggcctag gccccgacga
     3001 ggtgtcggtc caggcggttc tcggccgggt cgatcctgcc gatgcgctgg tcaatccgaa
     3061 gaccgtgccg atgctgcaca ccggcaccgc cgagggcgga aacgagatct tctcgacgac
     3121 cactccgctg cccgttgccg gtcccgtcgg ctatacggtg cgcgtcctac cgcatcatcc
     3181 gctgctggcc tccgactccg aactcggact ggtcaccctg gcctgacatc ggaccgattc
     3241 gtcggacttc gggtcggcac gttggggcga ctacggctca ggggaaccgg ttgaagcagc
     3301 ggtcctcgtc gccgagcacg cccgtgcgga acgcgtccat ccgggagaaa ccggacggca
     3361 ccgtgtcgcc gttcacgtcg ctggccgcga gcccgttggt gagcagaccg gagacggcct
     3421 cgtcgaggtc gcccgccgtc aacgccaccg tattgccgtc cgacgtggtg accgctgcgg
     3481 tcatcttggt cgtcgcgacg ccggtcaggc atgcagtgcg cagcgcggct tcggcgttgt
     3541 cgagacgcac gccggcgcgc tggtgctgaa gggccagcat gtagcgggag acgaggaccg
     3601 agtacgctgt gttgtcaccg gtcaccgcac cgccgttctc gtcctgcccc gatggtgcgc
     3661 ccatcgacgc cagtttgggc atatcgacgg agatcgtgtt gctggccggg caataggaca
     3721 ccggcgggct ggggcgcgcg tcgtggcaat cgttcgccga acccgcgtcg aggctcagct
     3781 tgggtgggtc ggccggtgag aacagcgtct cgagcgcttc gacgatggcc ttcaccgact
     3841 cctcgctgac cggccattcg cccgtctggt cctgctgcag ctcgacgggc aggtcgccgc
     3901 gacgctgtgc gatctccttg gcgtcgatgg ccgcgcacga cgatgccccg tcggtgaagc
     3961 cgaactggaa cgcggtgacc cgttcgaatg ccgaaccgtg ctcgttgccg ccgggtgcgt
     4021 actggtcctc gctcagcagc gggtcgcgga acgagatcag cgctgccaga agcctgttga
     4081 ggccgtctgc ggtactcagc gtgaagcgag cggaattgcc ctcggccacc cagcgcatgt
     4141 acgcgccggc aaagcagtcc gcctgctgct cggcgaccag cgtcgggatg ttcttcttgt
     4201 tgattttggc ctgatgagca atggcatgcc cgtattcgtg ggccagcaca ttggtgactg
     4261 cgatgtcgcc gaagttgtcc cgcagcgcag gaagcagctc accgcggtcc cacccgatgg
     4321 tgttgtcgag aaagcagtag ccggcgttga ccaagccgta cgtgtcttct tcgcagaact
     4381 cctggctctc gtaaccgcct gcgtcccagg agatcagcgc ctcgaccggg gagaagctcg
     4441 tgccgaacgt ctcggcatat gtggtttccc agaagtcttc gatgtcgctg accgagtccg
     4501 cgcccagttc gtcgacctcg ccgccgtcac cgttctcgac gtcgcgcgac gccttcttcg
     4561 cgtcgggtcg cagccccgtc ggcccgtcca cggcgggcat gcccgccacc ttgaacggat
     4621 cgtcgaacac cgagaccgcg gtgcccttca ccgtcgtgga gcaggacgcc acgaacagtg
     4681 cggcgcagac ggcaagcaga gcaccggtca ccggtcgtcg gctcatcggc gtcacgatag
     4741 tgcggcgttg gcggcgtgga ggcgttccag cgcttgccgt acccgcaccg aatccgtggt
     4801 cgcccagaac ggcggcagcg acgccagcag atagccggcg taacgcgcgg tggccatccg
     4861 cgaatccagc acggctacca ccccgcggtc ctcggaacgc cgcaacagcc ggccggcgcc
     4921 ctgggccagc agcagtgcgg catggctggc cgcgacggcc atgaacccgt ttccaccgcg
     4981 cgcggcgacc gcgcgctggc gcgccgagag caggggatca tccgggcgcg gaaacggaat
     5041 gcggtcgatc agcaccaacg acagcgacgg ccctggcacg tcaacgcctt gccacagcga
     5101 caacgtgccg aacagacagg tcgccggatc gtcggcgaac tgcttcacca aggcggacgt
     5161 ggtgtcgtcg ccctggcaga gcaccggtgt ggacagccgc tctcgcatga tctccgcggc
     5221 cgcccgtgcg gcccgcatcg acgagaagag tcccagcgtt cg
//
LOCUS       DASYLX010000022         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2634407,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000022 DASYLX010000000
VERSION     DASYLX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634407"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /gene="lysX"
                     /locus_tag="VGP27_00650"
     CDS             <1..1726
                     /gene="lysX"
                     /locus_tag="VGP27_00650"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HEV7849859.1"
                     /translation="LYRSNMKYKPQWVPRYACYEDARLVPRVGVASVIAEGFLVLPFS
                     RRKQHTGQHIAAPKNLVATGLLHHDGSAPDLKELRADLPENGDAQRLPEQVRVRMAKL
                     KELQDSGVDPYPVGQPPSHTVAQATETADGSDVTMAGRVLRIRDYGGVLFAQLRDWSG
                     EIQLLFDNSHLDAGSTADFTGSIDLGDLLEVTGTMGKSRSGTQSLIVGSWRLIGKCLR
                     PLPDKWKGLTDQEARVRARYVDLAINTEARELIRARSGVLHAIRETLVGKGFLEVETP
                     ILQQIHGGANARPFLTHINAYDLDLYLRIAPELYLKRLCVGGVERVFELGRAFRNEGV
                     DFSHNPEFTLLEAYQAHADYNVWIDGCRELIQNAALAANGEQVFLRPRDDGTMEKVDI
                     SGQWTVKTVHGAVSEALGEQIGPDTDLTTLRKLCDAADIPYLTHWDAGAVVLELYERL
                     VEDRTEEPTFYKDFPTSVSPLTRPHRSTPGVAERWDLVAWGVELATAYSELTDPVEQR
                     RRLQEQSLLASGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRVVMLITGRSIRETL
                     PFPLAKPR"
     gene            1813..3141
                     /locus_tag="VGP27_00655"
     CDS             1813..3141
                     /locus_tag="VGP27_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7849860.1"
                     /translation="MHGWLPVTVQIIAALALIAAVSRRSGRWQVRWLPWALIVGAALA
                     ASAYWYVASQGLSDNPAPNALWVWIGLTGFAAAVLVAGWRRTRWWRRGVSLAAFPLSL
                     LCAALALNLWVGYFPTVQVAWNQLTSGPLPDQTDEVTVVAMQQQRKIPPKGTVVTVDT
                     GDPASGFRHRGELVYLPPAWYASNPPPKLPTVMMIGGEFNTPADWLRTGQAIKTIDEF
                     AAAHHGNAPVLVFVDPGGAFNNDTECVNGSRGNSADHLTKDVVPFMTSHFGVSPAGSN
                     WGVVGWSMGGTCAVDLATMHPDMFSAFEDIAGDIGPNSGNKEQTISRLFGGNANAWAA
                     FDPTTVITKHGPYAGVAGWFDVNGSAATIQRAKNDQVAAANSLCSLGAANGIDCALVS
                     QPGKHDWPFAARAFATALPWLAGRIGTPDVPPTPLPPVVVSPSIEQTAAR"
     gene            3316..>3563
                     /locus_tag="VGP27_00660"
     CDS             3316..>3563
                     /locus_tag="VGP27_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849861.1"
                     /translation="MSDEPNPEPAPVDSEPGAATPPPAQAADSGYTPDGVPTFESVRE
                     KIETRYGTAIGATELAAETPEGRSVEEQYEERQRAAAER"
BASE COUNT          625 a         1180 c         1176 g          582 t
ORIGIN      
        1 gctgtatcgc tcgaacatga agtacaagcc ccaatgggta ccgcggtacg cctgctacga
       61 agacgccagg ctcgtcccgc gcgtcggcgt cgcatcggtg atcgccgaag gctttctggt
      121 gcttccgttc tcgcgacgca aacagcacac cgggcaacac atcgcggcgc cgaagaatct
      181 ggttgcgacg ggtctgctgc atcacgacgg ctccgcaccg gacctcaaag agctacgggc
      241 cgacctgccc gagaacgggg acgcgcaacg gctgcccgag caggtacgcg tccggatggc
      301 aaaactcaag gagctgcagg acagcggggt tgacccctac cccgtcgggc agccacccag
      361 ccataccgtc gcgcaggcga ccgaaaccgc cgacggctcc gatgtcacga tggcggggcg
      421 tgtgctgcga atacgggact acggcggggt gctgttcgcg caactgcgcg actggtcggg
      481 agaaattcaa ctcctgttcg acaattcgca tctcgacgcc ggcagcacgg ccgacttcac
      541 cggttccatc gatctcggcg acctcctgga ggtgaccggc acgatgggca agagccggtc
      601 gggcacccaa tcgctgatcg tgggcagctg gcggctgatc ggcaagtgcc tgcgtccgct
      661 gcccgacaag tggaagggat tgaccgacca ggaagcacgg gtgcgcgccc gctacgtcga
      721 cctggccatc aacaccgagg cgcgagagct catccgcgca cgcagcggag tgctgcacgc
      781 catccgcgaa acattggtcg ggaagggctt tttggaggtc gagaccccga tcctgcagca
      841 gatccacggc ggcgcaaacg cccgcccgtt cctcacacat atcaatgcat atgacctcga
      901 cctgtatttg cgcatcgccc ccgagctgta cctcaagcgg ttgtgcgtgg gcggcgtcga
      961 gcgggtgttc gagctgggac gggcgttccg caacgagggc gtggacttca gtcacaaccc
     1021 cgagttcacg cttctggagg cataccaggc gcatgccgac tacaacgtgt ggatcgacgg
     1081 ttgccgtgag ctgatacaga acgccgcact ggccgccaac ggcgaacagg tgttcctgcg
     1141 tccccgcgac gacggcacca tggagaaggt cgacatctcc ggacagtgga cggtcaagac
     1201 ggtgcacggg gcggtctccg aagcgctcgg cgaacagatc gggcccgaca cggacctgac
     1261 gacattgcgc aagctgtgcg acgccgccga catcccctac ctcacccact gggacgccgg
     1321 cgcggtcgtg ctggagctct acgagcggct cgtcgaggac cgcaccgagg agcccacttt
     1381 ctataaggat ttcccgacgt cggtgtcgcc gctgacccgg ccgcaccgca gcacccccgg
     1441 ggttgctgag cggtgggatc ttgtggcctg gggtgtcgag ctggctactg cttacagcga
     1501 actcaccgac ccggtggaac agcgcagacg gctgcaggaa cagtcgctgc tggcgtccgg
     1561 cggcgatccg gaggcgatgg agttagacga ggactttctg caggccatgg agtacgcgat
     1621 gccgccgacg ggcggattgg gtatgggagt ggaccgggtg gtgatgctta tcaccgggcg
     1681 cagcatccgc gaaacgttgc ctttcccgtt ggccaaacct cgctagcagc tgactcacag
     1741 cgggccacaa gctaggcgcg ccaccatgtt gccgtgacgt tacccgactc gttgcgccac
     1801 cacctctccc tcatgcacgg ctggcttccg gtcaccgtgc agatcatcgc tgctctggca
     1861 ctgatcgcgg cggtcagtcg acgtagcggc cgctggcagg tgcgctggct gccgtgggca
     1921 ctgatcgtcg gcgccgccct ggccgcatcg gcctactggt acgtcgcgtc ccaggggctg
     1981 tccgacaacc ccgcgccgaa tgcgctgtgg gtctggatcg ggctcaccgg tttcgcggcc
     2041 gccgtcctgg tggccggctg gcgccggacg cggtggtggc gtcgcggcgt ttcgctggcg
     2101 gcgtttccgc tcagcctgct ctgcgccgcg ctggcactga acctgtgggt cggctacttc
     2161 cccaccgtgc aggtcgcatg gaaccagctg acgtccgggc cgttgccgga ccagaccgac
     2221 gaggtcaccg tggtcgcgat gcagcagcag cgcaagatcc cgccgaaagg caccgtggtg
     2281 accgtcgaca ccggcgatcc ggcgtcgggt ttccggcacc gcggggagtt ggtctacctt
     2341 ccgcccgcct ggtacgcaag caacccgccg cccaaactac cgacggtgat gatgatcggc
     2401 ggcgaattca acacccccgc cgactggctg cgtactggtc aggcgatcaa gaccatcgac
     2461 gagttcgccg ccgcgcacca cggcaacgcg ccggtgctcg tcttcgtcga ccccggtggt
     2521 gcgttcaaca acgacaccga gtgcgtcaac ggcagccgcg gcaacagcgc cgaccatctc
     2581 accaaagatg ttgtgccgtt catgacctca cacttcggcg tcagccccgc cgggtcgaac
     2641 tggggcgtcg tcggttggtc gatgggcggt acgtgtgcgg tcgacctcgc gacgatgcat
     2701 ccggacatgt tcagcgcgtt cgaagacatc gccggcgaca tcggaccgaa ctccggcaac
     2761 aaggaacaga ccatttcgcg gctgttcggc ggaaacgcga atgcgtgggc ggcgttcgac
     2821 ccgaccaccg tcatcaccaa acacggcccc tacgcgggtg tggccgggtg gttcgacgtc
     2881 aacggctcgg cggcgacgat ccaacgcgcc aagaacgacc aggtcgcggc ggcgaattcg
     2941 ttgtgctcgt tgggcgctgc caatgggatc gattgcgcgc ttgtgagtca gccgggtaag
     3001 cacgattggc cgttcgcggc acgcgcgttc gccactgcac tgccctggtt ggccgggcgg
     3061 ataggcacac ccgacgtgcc gcccaccccg ttgccacctg tggtggtctc cccgtcgatt
     3121 gaacagaccg ccgccaggtg accatcatca gtcaatgtcg accttcgact gtgaatgttc
     3181 gcatggacga gtcctcaacc tggcgatctt cggaagccgg cacacgatcg cgcataggca
     3241 ttccgagcgc gttcattgcc attggtggcg ggctatcggg accgacaccg catgtcggcg
     3301 gagtagcgtt gcgtcatgtc cgacgaaccg aatcccgaac cggctcctgt cgactcggag
     3361 cccggcgccg cgactccgcc acctgcccag gccgccgaca gtgggtacac gccggacggc
     3421 gtgccgacct tcgagtcggt gcgggagaag atcgagacgc gctacggcac tgcgatcggg
     3481 gccaccgagc ttgcggcgga gacacctgaa gggcgctctg tcgaggagca gtacgaggag
     3541 cgtcagcgcg cggccgcgga acg
//
LOCUS       DASYLX010000023         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1318088,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000023 DASYLX010000000
VERSION     DASYLX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318088"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGP27_00665"
     CDS             <1..297
                     /locus_tag="VGP27_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7849862.1"
                     /translation="EIPMVILIWDDNAYGLISWKMDLEIGHNVDTRFGNPDFVAYAES
                     FGAKGYRISAAEELLPTLRAALDDDTVSIIACPVDYSANSDLIASLGELDESLS"
     gene            complement(383..1249)
                     /locus_tag="VGP27_00670"
     CDS             complement(383..1249)
                     /locus_tag="VGP27_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7849863.1"
                     /translation="METIDLAYVGRGIHEELVAHVADQEGYFADEGIHVAIHDGTGWD
                     DDRLRSCATIGLGRAVLSRLTDGVEWTVHSVNTHRPLFWFLGGGDVASMADLRGRRLA
                     VHAPHTAPGCFARIVLRRHSIDPDNDLVCVSRHPGDYQMDLRRLRDGSIDAAYVGSTL
                     GPEQIAEEEGFHVLAWVGDDFQIPTVGIAADAAHFKSDDAALQALVRANQRALKTLAD
                     TPDLAVGYLASFLNRMTRDEVQRYYERYIGPYFGSDGRVDRDIARAAIDAVAHELGVA
                     SGPAEQIYQPAR"
     gene            complement(1295..2038)
                     /locus_tag="VGP27_00675"
     CDS             complement(1295..2038)
                     /locus_tag="VGP27_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849864.1"
                     /translation="MDRVAVITGASRGIGAATALVLAERGFRVVVNYRSSADEAQQVV
                     RAATDAGGEAMAVRADVTQGDEVAKMVAEAENRWGRIDVLVHNALIPFDVTSFADLTW
                     EQLGGKLERELRAAYLITKAIVPGMISRKYGRMVFLSTGLSKRPRDGMIALGTAKAAM
                     DQFVRYIALELAPHGITANLVAPATVDETTVTDQLTPQQMEHLGASNPMGRLVRPDEV
                     GKTVAFLASEDSGFTTGAYLPVNGGLAMD"
     gene            2230..3021
                     /locus_tag="VGP27_00680"
     CDS             2230..3021
                     /locus_tag="VGP27_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849865.1"
                     /translation="MSQWLVSSMPSWLLLLTLIAVTTGLAIAVQIVVRRRYPKLRGEE
                     HNDVTKFAFGVVGFVFAFFIGFVVSAMWGQINDADGRARTEGAAGVELARSVTIFDQP
                     DRDRIRQSLLDYENAAVIEWSEAAGGRHYPEADKALARLRSAYEGVQTLSRAQETVLA
                     MSFANLDSLSQARTQRVLQARTDIGPPWSLWAVIFLTGGLLLGCAIIYGVEKHTTHFT
                     MVAILGVLVGAELFLVLELSHPFIGEIATSPEPLREVIRALSSNP"
     gene            complement(3048..3407)
                     /locus_tag="VGP27_00685"
     CDS             complement(3048..3407)
                     /locus_tag="VGP27_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2640c family ArsR-like transcriptional
                     regulator"
                     /protein_id="HEV7849866.1"
                     /translation="MPKTLPVIDMSAPVCCAPMAAGPMSDEAALQVALRLKALADPAR
                     VKIMSLLFGSVHGEENSGDLAAVLELTESTVSHHLAQLRKAGLVESQRRGMNVYHRAH
                     RDALGALCAVLDPTCCT"
     gene            3506..3979
                     /locus_tag="VGP27_00690"
     CDS             3506..3979
                     /locus_tag="VGP27_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEV7849867.1"
                     /translation="MSRVQLALNVDDLDQAITFYSKLFNTEPNKVKDGYANFAVVQPP
                     LKLVLLQNPGKGGSINHLGVEVDSSTEVHSEIARLSEEGLFTDEEIGTTCCFATQDKV
                     WVTGPAGEKWEVYTVLADSETFGSSPGHLDSRSEGVCCGSQADDTRKASSATSCC"
     gene            complement(4037..4711)
                     /locus_tag="VGP27_00695"
     CDS             complement(4037..4711)
                     /locus_tag="VGP27_00695"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEV7849868.1"
                     /translation="MSDSPVSLAGHLRRDLSIDQQHAVKTAATRLHTEFGEHFSIETI
                     ERFLHSSYDQFAGRAAIPNFLALLAERWARQRLNALARVEGKVTEATPTVLFLCTHNA
                     GRSQMALGYFNHLAAEQGVAWSGGSEPGNEINPSAVQAMAEVGIDITREFPKPWTDEI
                     VRAADVVVTMGCGDECPFFPGKRYENWELADPAGQSVDTVRPIRDEIEERVRRLLTEL
                     GITPVS"
     gene            complement(4768..5817)
                     /gene="arsB"
                     /locus_tag="VGP27_00700"
                     /pseudo
     CDS             complement(4768..5817)
                     /gene="arsB"
                     /locus_tag="VGP27_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
     gene            complement(5814..6197)
                     /locus_tag="VGP27_00705"
     CDS             complement(5814..6197)
                     /locus_tag="VGP27_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7849869.1"
                     /translation="MSKSRLPLSDVSACAYAPLVREPLSPAAAAEVAAKLKALSDPVR
                     LRLFSLVASHVGGEACVCDISGDFDVSQPTISHHLKVLKDAGLLASERRGSWVYYSVV
                     SEALINLASVLSTSADDDVTTGSPT"
BASE COUNT         1243 a         2000 c         2060 g         1170 t
ORIGIN      
        1 gaaatcccga tggtgatcct catctgggat gacaacgcct acggtctgat cagctggaag
       61 atggacctgg agatcggcca caacgtggac acccgattcg gcaatcccga cttcgtggcc
      121 tatgccgaga gtttcggcgc aaagggctac cgcatctcgg ccgccgagga actgcttccg
      181 accctgcgcg ccgcactcga cgacgacacc gtcagcatca tcgcgtgtcc cgtggactac
      241 tcggccaaca gcgacctcat cgcttcattg ggcgagttgg acgagtcact gtcctgaagc
      301 gctgcactac ggccacggtg ctcgacagcc gaggagccaa gctgcacttc tccgttccgg
      361 gctccgacgt cgtgctcctc ggtcatcggg cgggttgata gatctgctca gcgggccccg
      421 aagcgacacc cagttcgtgt gcgacggcgt cgatggcggc tcgggcgata tcgcgatcca
      481 cccggccgtc ggagccgaag tacggtccga tgtagcgctc gtagtagcgc tgcacttcgt
      541 cgcgggtcat ccggttcaga aatgatgcga ggtagccgac ggccagatcg ggtgtgtccg
      601 caagcgtctt cagggcgcgc tggttggccc ggaccagcgc ctgcaacgcg gcgtcgtcgg
      661 atttgaagtg ggccgcatcc gccgcgattc ccacggtggg gatctggaaa tcgtcgccta
      721 cccaggcgag cacgtggaag ccctcctcct cggcgatctg ctcgggccca agggtgcttc
      781 cgacatacgc ggcgtcgatg ctgccgtcgc gaaggcgtcg caggtccatc tgatagtcgc
      841 ccggatgccg cgacacgcac acgaggtcgt tgtcggggtc aatactatga cggcgcaata
      901 cgattcgcgc gaagcatccg ggcgccgtgt gcggcgcgtg taccgcgagt cggcgccctc
      961 gaagatccgc catggacgcc acgtcgccgc cgccgagaaa ccagaagagt ggtcgatggg
     1021 tgttgacgct gtgcacggtc cattcgacac cgtccgtcag acgagacagc acggctcgtc
     1081 cgagcccgat cgtcgcgcac gaccgcaggc gatcgtcgtc ccagccggtt ccgtcgtgga
     1141 tggcgacatg gatgccttcg tctgcgaaat agccctcctg atcggcgaca tgggccacca
     1201 gttcctcatg aatgccacgt ccgacgtagg caaggtcgat cgtttccatg atgcgattcc
     1261 ttcgctcgat ctgatgagaa cgacgcagcc gcggtcagtc cattgcaagg ccgccgttga
     1321 ccggcaggta cgcaccggtg gtgaaacctg agtcctcact cgccagaaac gcgaccgttt
     1381 tgcccacctc gtcgggccga accagccggc ccatgggatt ggacgcgccc aagtgctcca
     1441 tctgttgggg agtcagctgg tcggtcaccg tcgtctcgtc caccgtggcc ggcgcgacga
     1501 ggttggcggt gatcccgtgt ggcgccagct cgagcgcaat gtaacggacg aactggtcca
     1561 tggcggcctt ggcggtgccc agcgcgatca tcccgtcccg cggccgtttc gacagacccg
     1621 tgctgaggaa aaccatgcgt ccgtacttcc gggaaatcat ccccggcaca atcgctttgg
     1681 ttatcaggta ggcagctcgc agctcgcgct ccaacttgcc gccgagctgt tcccaagtga
     1741 gatcggcgaa cgacgtgacg tcgaagggga tcaacgcgtt gtgaacgagt acgtcgatcc
     1801 ggccccatcg gttttcggct tccgcgacca ttttggccac ctcatcgcct tgggtcacgt
     1861 cggcccgcac cgccatcgct tcgccgccgg cgtccgttgc ggcacggacc acctgctgcg
     1921 cctcatcggc gctggagcgg tagttgacga cgacgcggaa tccgcgctcc gcgagaacga
     1981 gtgctgtcgc cgcaccgatt ccgcgactgg cccccgtgat gaccgcaaca cggtccatgt
     2041 gggtccacct cctgatcatg agaatgccac ccacactcgt ggttgggcga tggattcgcg
     2101 cgagcagaac tcacaggctc gctaggtctg tgcgccggac tcgaaattcc agatcaccgc
     2161 tccccgaggg caaccgacac ttaaggtcga gaagccccag acgcgtgaac ttccgagagg
     2221 agtgcggctt tgagtcagtg gttggtcagc agtatgccgt cgtggctgct tctgctcaca
     2281 cttattgcag tcacaactgg tctggcaatc gccgtccaga tcgtcgttcg gaggcgatac
     2341 ccgaagctcc ggggagaaga gcacaacgac gtcaccaagt tcgctttcgg tgtcgttggc
     2401 tttgtcttcg cattcttcat cggtttcgtc gtatcggcga tgtggggcca gatcaacgat
     2461 gccgacggta gggcgcggac cgagggcgcc gctggggtgg aattggcgag aagcgtcacc
     2521 atattcgacc agccggatcg cgaccgaatt cgacagagcc tcttggacta tgagaacgcc
     2581 gcggtcatcg aatggagcga ggccgccggt gggcgtcatt atcctgaagc cgacaaagca
     2641 ctggcccgtc tgcgttccgc ctacgaaggt gtacagaccc tctcgcgcgc acaggagacc
     2701 gtgctggcca tgtcgttcgc gaatctcgac agcctcagcc aagcccgcac tcaaagggtc
     2761 ctacaggcgc gaaccgacat cgggccgccg tggtcgctct gggcggtcat tttcttgacc
     2821 ggcggactac ttctcggctg cgcgatcatc tacggcgtcg agaagcacac cacgcatttc
     2881 acgatggtcg cgatactcgg tgtgcttgtc ggggccgagc tgtttcttgt cttggagctt
     2941 tcgcatccgt ttatcggcga aatcgcaacg tcgccggaac cactacgcga agtcatccgc
     3001 gccctatcgt cgaatcctta aaggacgttc ggggccacgc tgactagcta tgtgcagcag
     3061 gtggggtcca agacggcgca aagcgcgccg agtgcgtcgc ggtgcgctcg gtggtatacg
     3121 ttcatgcccc gccgctggga ctcgaccaag cctgccttcc gtaattgagc cagatgatgg
     3181 ctcacagtgg attccgtcag ctccaaaaca gccgcaaggt caccactgtt ctcttcgccg
     3241 tgaacagagc cgaacaggag cgacatgatc ttcactcgcg ccgggtcagc cagcgccttc
     3301 aaccgcaggg ctacttgaag cgccgcttcg tccgacatcg gaccagcggc catcggggcg
     3361 cagcagaccg gtgcggacat gtcgatcacc ggcagagtct tcggcatagc ccgaccatac
     3421 ccagtttctt gacatatatc aaagaggtcg gcattctgta gacggcgcca gttcgatata
     3481 tgtctcacac ctgaaaggac tgcacatgtc ccgcgtccaa ctcgccctca atgtcgatga
     3541 cctcgaccag gcgatcacgt tttactcgaa gctgttcaac accgagccca ataaagtcaa
     3601 agacggctac gcgaacttcg ctgtcgtcca gccgccgctc aagctggtac tcctccagaa
     3661 ccccggcaag ggcggctcca tcaatcacct tggggtagag gtggattcga gcaccgaggt
     3721 gcactctgaa atcgcgcgcc tcagcgagga aggactgttc accgacgagg agatcggcac
     3781 gacgtgttgc ttcgccaccc aggacaaggt atgggtcacc ggccctgcgg gtgaaaagtg
     3841 ggaggtctac accgtgctcg ccgactccga aaccttcggt tccagccctg ggcacctcga
     3901 ttcccgcagc gaaggcgtgt gctgcggtag ccaggcagat gacactcgga aggcttcgag
     3961 cgcgacgtcg tgctgctgac gacaattcca tcggagctcc cgcgggcgta gacgtctgcg
     4021 tccgcgggcc cgctcatcag gagaccggcg tgatgccgag ctcggtcaac aggcggcgaa
     4081 cgcgctcctc gatctcgtcg cggatcggac ggacggtgtc gacagactgt ccagcggggt
     4141 cggccaactc ccagttttcg taccgcttac cggggaagaa cgggcattca tctccgcacc
     4201 ccatggtgac gacgacgtcg gccgcccgga caatctcatc ggtccagggc ttggggaact
     4261 cgcgggtgat gtcgatgccg acctcggcca tggcctgtac tgccgatggg ttgatctcgt
     4321 tgcctggctc ggaccctccc gaccaggcga cgccctgctc ggctgcgagg tggttgaagt
     4381 acccaagagc catctgagag cggccggcgt tgtgggtaca cagaaacagc acggtcggcg
     4441 tggcttcggt gaccttgccc tcaacccggg ccagagcgtt aaggcgttgg cgcgcccaac
     4501 gttccgccaa caacgccagg aagttgggga tggcggcacg gcctgcgaac tggtcgtagg
     4561 aggaatgcag gaaacgttcg atggtctcga tgctgaaatg ttcgccgaac tcggtgtgca
     4621 gacgagtggc ggcggtcttc acagcatgct gttggtcgat ggacaggtcg cgccttaggt
     4681 gaccggccag ggaaacaggg ctgtcgctca ccgtgcgctc cgatccgtga tcagtgttgt
     4741 tttcgggttg tcaagggtgg atcggcgacg catggcgagg gagacataga ccagggcgac
     4801 gaggaccggg acttcgatga gcgggccgac cacacctgcg agagcttgac ccgaggtcgc
     4861 gccgtaggtg gcgatcgcca ctgcaatggc caattcgaag ttgttgcccg ctgcggtgaa
     4921 cgccagggtg gtgatgcgcg cgtacccgag gttcagcaga gcgcccagga ggtagccgcc
     4981 accccacatg atggcgaagt acgccagcag cggcagggcg atgcggacca cgtcaagcgg
     5041 gcggctggtg atctgctcac cttgcagcgc gaagaggatg acaatggtga acagcaggcc
     5101 atacagcgcc cacgggccga tcttcggcag gaatgcggat tcgtaccacg ttcggcccct
     5161 ggccttttcg ccgagccgtc gggaaagata cccggccagc agtgggatgc cgagaaagat
     5221 gaccactgac ttggcgattt tccacggcga ggtgctgatg gtggtctgtt ccaggcccag
     5281 ccagccgggc agtaccgaga ggtagaacca tccgaggacg gcgaacatga tcacctggaa
     5341 cattgagttc agcgcgacga ggacggcggc ggcttcccgg tctccgcagg cgaggtcgtt
     5401 ccagatgatc accatggcga tgcagcgcgc caaccccacg atgatcaggc cggtgcggta
     5461 ttcaggaagg tccgacagca gcagccacgc aagggcgaac ataagtgccg gaccgatcac
     5521 ccagttgagc agcaaggagc tgagcagtag cttgcggtcg ccggtgaccg tgtcgagtcg
     5581 gtcgtagcgg accttggcga gcaccgggta catcatgacc agcaagccga gtgcgatggg
     5641 caacgaaata ccgtcgactt caacatgatt gagcgcattg ttcaagccgg gcacgacgcg
     5701 tccgaggaac agcccggctg ccatcgccat gccgatccac agcggcagat accggtcgag
     5761 caacgacagc ttctcgacga cggctgggtg cgcgtccgag gtgaccggct tggtcatgtc
     5821 ggtgatccag tcgtgacgtc gtcgtcggcg gacgtgctca gtaccgatgc caggttgatc
     5881 agcgcctccg agacgacgga gtagtacacc caggaccctc ggcgctcgga ggcgagaaga
     5941 ccggcgtcct tgagcacctt gaggtgatgg gaaatggtcg gctgcgaaac atcgaagtcg
     6001 ccggagatgt cgcacacaca ggcttctcct ccgacgtgac tggcgaccag gctgaacaga
     6061 cgcaggcgca ccggatctga tagcgccttc agtttggcgg cgacttcagc ggcggccgca
     6121 ggtgacagcg gctcgcgtac caacggggcg taggcacagg ccgacacatc gctcagcgga
     6181 agacgtgact tagacacaca tcgatattga cactcttcta ttcaagacac aaacgaacag
     6241 tgtgtgaatc gaaccgactc gtcgcctgca ggccaccatg gcgcggtatc ggtgaacata
     6301 acgtagcttc gccttgagca ttccgccgaa cgcggccacc acgccggagc ggtgttgacg
     6361 agtgacgact ggagtgaagc catgagcggc cggggcccgg attctcgcac tgttccgcgg
     6421 tccggggtgg tggcgtgggc gctgtgggac tgcggcgcaa cgggtgtgaa cgc
//
LOCUS       DASYLX010000024         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2524667,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000024 DASYLX010000000
VERSION     DASYLX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524667"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="VGP27_00710"
     CDS             <1..2377
                     /locus_tag="VGP27_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849870.1"
                     /translation="RAETALTVLAPRREQLTGDEAHDRVRAILDETVDVTLWQAQRVL
                     QAASTAAVDLSGCDALSRALDVAAGDAVQLAGAEPSPASGRCPQLIDRIDAEYLRYFT
                     PTGRPTGEWATATKRMRDADDEVARCAAAVAEVDDAVRTHATLTAELERLAGERAEAV
                     AKLAAAQTAADEVAALTQQLKQAEVVAAAAAATREASVSALTERRRLQADVNERAAAV
                     AELERAAAQAHDDELTANEVQEAAETAAEEARAFLQASQARVEAARRAVERLSDRDEA
                     NRLSSRLTKIDAARREIEHVERELAPIALTDALMRDIETAAAAVERAAGQAELASAHI
                     EVVAVAGVELQVGGQPVALEAGGAWSANATTTTDIDVPGVLTVRVVPGAPAADTQAKL
                     DAAQDVLTTALAAAEVDGVTQARALDQRRRELVGARDRSTATANGLIGDDDVDALRAR
                     LAELRNGDIAHPDDTTGPADSASARVELNEASAAQRKAIADCETHRKVAEAAAKRLGE
                     RATHAGVAREKLTACYAELAVGRERLTQQRAAATDDELLMRAEADTDKARRATGLVAD
                     LGGELARCAPDTVTAAMDEALRRVDALCGRHDEVADALRLVTAQLKVYGTEGRKCQLD
                     AAETEREHAEAEYLRVHRRARAAQLLRSVMARHRDATRLRYVDPFRNQVERLGRIVFG
                     DTFEVEVDAELRICSRTLSGRTVPYDSLSGGAKEQLGIVARLAGAAMVAKEDSVPVVI
                     DDALGFTDPDRLIKMGAVFDAVGSDAQVIVLTCSPERYRSVGAAHHIDLSA"
     gene            complement(2385..3053)
                     /locus_tag="VGP27_00715"
     CDS             complement(2385..3053)
                     /locus_tag="VGP27_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7849871.1"
                     /translation="MAARKSYNQNCPIARGLDLLGERWTLLILRELIGGARRYSDLRA
                     ELPGIATNLLADRLRELEESGMVDRTELPPPIARTVYTLSEVGWRKVPPVIGAIATFG
                     LDLIEPDASALTPLNGFLAGILLAFDPARAADLAATYRVDIDGRRFEFGVRDRNLTAG
                     RMEPAITVAATAADLITARLGSTAAERKAALRRVKFDGEPDGVNAMRNVFRLAGDPGL
                     AVAT"
     gene            3131..>3672
                     /locus_tag="VGP27_00720"
     CDS             3131..>3672
                     /locus_tag="VGP27_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7849872.1"
                     /translation="MTTDPRPPVIRDRPIAALLVICLSVFVISVDATIVNVALPTLSR
                     ELDADTAQLQWIVDAYTLVMAGLLLSAGSLSDRYGRRGWLSSGLALFAITSAVAAQMT
                     TADGLIAARAAMGIGAAVIFPTTLALITNIFGDPAARAKAIGLWAAMVGVGVAVGPIT
                     GGWLLEHFAWGSIFWVNVPVA"
BASE COUNT          589 a         1277 c         1292 g          514 t
ORIGIN      
        1 acgcgcggag accgcgctca cggtgctggc gccgcggcgc gaacagctga ccggcgacga
       61 ggcacacgac cgggtgcggg cgatcctcga cgaaaccgtc gacgtgacgc tgtggcaggc
      121 gcaacgcgtg ttgcaggcgg cgtcgacggc cgcggtggac ctgtcgggat gcgacgcgct
      181 gtcccgggcg ctcgacgtgg ccgccggtga cgcggtccag ctcgctggcg ccgagccgtc
      241 ccccgcaagc ggcaggtgcc ctcagctgat cgaccgcatc gacgccgagt acctgcggta
      301 cttcacgccc accggccgcc cgaccggcga gtgggccacc gcgacgaaac gcatgcgaga
      361 cgccgacgac gaggtggccc gctgcgcggc cgcggtcgcc gaggtcgacg acgccgtacg
      421 cacgcacgcc acgctcaccg cggaactcga gcggctggca ggtgagcgcg cggaagcggt
      481 cgcgaagctg gccgccgcgc aaaccgccgc cgatgaggtc gcggcgctga ctcaacagct
      541 caagcaggcg gaagtggtcg ccgccgccgc cgctgccacg cgggaagcgt ccgtctcggc
      601 gctgaccgag cggcgcaggc tgcaggccga cgtcaacgag cgcgccgccg cggtcgccga
      661 gctggagaga gcggccgcgc aggcccacga cgacgaactc accgccaacg aggttcagga
      721 ggccgcggag acggccgccg aagaggcccg tgctttcctg caggccagtc aggcccgcgt
      781 cgaggccgct cgccgcgccg tcgagcggct gtcggaccgc gacgaggcca accggctgtc
      841 cagccggctg accaagatcg acgccgctcg gcgcgaaatc gagcacgtcg agcgcgaact
      901 cgcgccgata gcgctgaccg acgcgttgat gcgcgacatc gagaccgcgg cggccgccgt
      961 ggaacgcgca gccggtcagg ccgagctggc gtcggcgcac atcgaggtcg tcgcggtcgc
     1021 aggcgtcgag ctgcaggtcg gcgggcaacc ggtcgccctg gaggccggcg gcgcctggtc
     1081 ggcgaacgcc accacaacga cggacatcga cgtgccgggt gtgctgaccg tgcgggtggt
     1141 gcccggcgcg cccgccgccg acacgcaagc caaactcgat gctgcacagg acgttctgac
     1201 gactgcgctg gcagccgccg aagtcgacgg tgtcacgcag gcgcgcgccc tcgatcagcg
     1261 gcgccgggaa ctcgtcggtg cgcgcgacag gtcgacggcg accgccaacg gcctcatcgg
     1321 tgacgacgac gtcgatgcgc tccgcgcgcg gctcgccgag ctgcgcaacg gcgacatcgc
     1381 acaccccgat gacacgaccg gcccagccga ttcggcgagc gcccgagtcg agctcaacga
     1441 agccagcgca gcgcagcgca aggccatcgc cgattgcgag acccatcgaa aggtggccga
     1501 ggcagcggca aagcgcttgg gggagagggc aactcacgcc ggtgttgccc gcgagaagct
     1561 gacggcgtgc tatgcggagc tggccgtggg acgggaacgc ctgacacagc agcgcgccgc
     1621 cgcgaccgac gacgagcttc tcatgcgggc cgaggccgac acggacaagg cgcgccgcgc
     1681 caccggcctc gtcgcagacc tcggcggcga actcgcccgc tgcgcaccgg ataccgttac
     1741 cgccgcaatg gacgaggcgc tgcggcgcgt cgacgcgctg tgtggtcgtc atgacgaggt
     1801 cgccgacgca ctgcggctgg tgaccgcaca gctcaaggtg tacggcaccg agggacgcaa
     1861 atgccagctc gacgccgccg aaaccgaacg cgagcatgcc gaagccgaat acctgcgggt
     1921 gcaccggcgg gcgcgggccg cacagctgct gcgctcggtg atggcccggc atcgtgacgc
     1981 cacccggctg cgctacgtgg acccgttccg caatcaggtc gagcggctgg gccgcatcgt
     2041 cttcggggac accttcgaag tcgaggtcga cgccgagctg cggatctgta gtcgcacgct
     2101 gtcgggacgc accgttccct acgactcgct gtccggcggc gcgaaggagc agttgggcat
     2161 cgtggcaagg ctggcagggg ccgccatggt cgccaaggag gacagcgttc ccgttgtcat
     2221 cgacgacgcg ctcggcttca ccgaccccga ccggttgatc aagatgggcg cggtgttcga
     2281 tgccgtcggc agcgacgccc aggtcatcgt gttgacctgc agcccggagc gttacagaag
     2341 cgtcggcgcc gcgcaccaca tcgacctgtc ggcgtagctg caacctaggt ggcgaccgcc
     2401 aagcccgggt cgccggccaa cctgaacacg ttgcgcatgg cgttgacgcc atcgggctcg
     2461 ccgtcgaatt tcacccgccg cagcgcggcc ttacgctcgg cggccgtcga gccgagcctg
     2521 gcggtgatca ggtcggcagc cgtcgcggcc accgtgatcg ccggttccat ccgtccagcg
     2581 gtcaggttgc ggtcacgcac gccgaattcg aagcggcgcc catcgatgtc gacccggtag
     2641 gttgccgcca ggtccgcggc gcgggcgggg tcgaacgcca acaggatccc ggccaggaag
     2701 ccgttcaacg gagtcaaggc gctcgcgtcc ggttcgatca ggtccaggcc gaatgtggcg
     2761 atggcaccga taacaggggg aaccttgcgc cacccgacct cgctgagggt gtacacggtg
     2821 cgggcgatgg gcggtggtag ctcggtgcgg tcgaccatgc ctgactcttc cagttcccgc
     2881 agccggtcgg cgagcaggtt ggtcgcgatg ccaggaagct cggcgcgcag gtcgctgtac
     2941 ctgcgggcgc ctccgatcag ctctcgcagg atcagcaagg tccatcgctc gccgagcaga
     3001 tccaggcccc gcgctatcgg gcagttctgg ttgtagctct tccgggcggc catacggaca
     3061 atctaccgta tggacaagtt tagagttcta ttgcttgaaa atcttgtcta taaggtttac
     3121 ggttcgacct atgactactg acccacgccc acctgttatc cgcgatcgcc cgatcgccgc
     3181 gctgctcgtc atctgcttga gcgtgttcgt catcagcgtc gacgccacca tcgtcaacgt
     3241 cgccctgccc acgctgtcgc gcgaactcga cgccgacacc gcgcaactgc agtggatcgt
     3301 cgacgcgtac accctggtaa tggcgggtct gctgctgtcg gcgggcagcc tgagcgatcg
     3361 ctatggccgg cgcggctggc tgagttccgg ccttgccctc ttcgccatca cctctgccgt
     3421 cgcggcgcag atgaccaccg cggacggcct gatcgcggcc cgggcggcga tgggtatcgg
     3481 cgccgcggtc atcttcccga cgacgctggc cctcatcacc aacatcttcg gcgatcccgc
     3541 cgccagggcg aaggccatcg gcctgtgggc ggcgatggtg ggcgtcggtg tggccgtcgg
     3601 cccgatcacc ggtggttggc tgctcgagca cttcgcatgg ggatcgatct tctgggtcaa
     3661 cgttccggtc gc
//
LOCUS       DASYLX010000025         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3292886,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000025 DASYLX010000000
VERSION     DASYLX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292886"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGP27_00725"
     CDS             complement(<1..528)
                     /locus_tag="VGP27_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV7849873.1"
                     /translation="MTIAVTGSIATDHLMRFPGKFSEQLLPEHLQKVSLSFLVDDLVI
                     HRGGVAGNMAFAIGVLGGDVALVGAVGKDFDDYRSWLEAVGVDTESVLVSDSAYTARF
                     VCTTDQDMAQLASFYPGAMSEARGISLADVVKRVGTPDLVIVGANDPEAMFLHTEECR
                     ELGLAFAADPSQQLAR"
     gene            624..1265
                     /locus_tag="VGP27_00730"
     CDS             624..1265
                     /locus_tag="VGP27_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849874.1"
                     /translation="MAGPYPPQYGPGPVGAPAPHQGPPQQYPDNPLAPPIYPGMLPPP
                     IPYPKRSRWRPILVTLVALAAVAAAVAATVLVMRSDENSTPERLTTESAQTAIQDYLN
                     ALQRGDVETIARHTLCGLFDAVKEKKSDLALADLSSDAFRRQFSAADVTSVDKMVFSS
                     PYQAQVLFTMRVTPAGSRSQPQPKVQEQAVAQLLAQDDQVLVCSYLLRAGSQY"
     gene            complement(1267..1629)
                     /locus_tag="VGP27_00735"
     CDS             complement(1267..1629)
                     /locus_tag="VGP27_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEV7849875.1"
                     /translation="MTVQDESATSTATHGAALTDAAASKAKALLDQEGRDDLALRIAV
                     QPGGCAGLRYNLFFDDRTLDGDLTTEFGGVNLTVDRMSAPYIQGATIDFVDTIEKQGF
                     TIDNPNATGSCACGDSFN"
BASE COUNT          300 a          592 c          557 g          313 t
ORIGIN      
        1 tcgggcgagc tgctgcgacg ggtcggccgc gaatgcgagg ccgagctcgc ggcactcctc
       61 ggtgtgcaag aacatcgcct cggggtcgtt ggccccgacg ataaccaaat ccggtgtgcc
      121 gacgcgcttt accacatcgg cgagggagat gccgcgggct tccgacatgg cgcctggata
      181 gaacgacgcc agttgcgcca tgtcctggtc ggtggtgcag acgaaccgcg cggtgtacgc
      241 gctatcggag acgagcacac tctcggtgtc gacgccgacg gcttcgagcc agctgcggta
      301 gtcgtcgaaa tcctttccga cggccccgac gagcgccacg tccccgccca gcacgccgat
      361 cgcgaaggcc atgttgcccg cgacgccgcc ccggtggatc accagatcgt cgaccagaaa
      421 gctcaaagac accttctgca ggtgctcggg cagaagctgt tcggagaact tgccggggaa
      481 ccgcatgaga tggtcggtcg cgatcgatcc ggttaccgcg atggtcacga agcctcaccc
      541 ttcaattcat taacctgtct agataattct accgagcaca atggtcgcgt cgccaaccgg
      601 ccgcttgcgc taacctgcct accatggctg gtccgtatcc gccccaatac ggccctggac
      661 cggtcggcgc tcccgcccca catcaggggc cgccgcagca gtaccccgac aatccccttg
      721 cgccgccaat ctatccgggc atgctgccgc cgccgattcc gtatccgaag cgcagccgct
      781 ggcgcccgat cctcgtgacg ctggttgcgc tcgctgcggt ggccgccgcc gtcgccgcaa
      841 ccgttctcgt gatgcgctcc gacgagaact cgacgcccga gcggctgacg acggaatcgg
      901 cgcaaacagc gattcaggat tacctcaacg cgctgcaacg cggcgacgtc gagacgatcg
      961 cccggcacac gctgtgcgga ttgttcgacg ccgtcaagga gaagaaatcg gacctcgccc
     1021 tggccgacct gagcagtgac gcgttccgca ggcagttcag cgccgcggac gtgacgtcgg
     1081 tcgacaagat ggtgttctcg tcgccgtatc aggcgcaggt gctgttcacc atgcgcgtga
     1141 cgccggcggg cagccgcagc cagccccaac cgaaggtgca ggaacaggcg gttgctcagc
     1201 tactcgccca ggacgaccag gtcctggtgt gctcgtatct gctgcgcgcg ggctcccagt
     1261 actagatcag ttgaacgagt ccccgcacgc gcacgaaccc gtcgcgttgg ggttgtcgat
     1321 cgtgaagccc tgcttctcga tggtgtcgac gaagtcgatc gtcgcgccct ggatgtaggg
     1381 ggcgctcatc cggtcaacgg tcaagttgac gccgccgaac tcggtggtca gatcgccgtc
     1441 gagggttcga tcatcgaaga agaggttgta gcgcaggcct gcgcatccgc ccggctgaac
     1501 ggcgatccgc agcgcaaggt cgtcacggcc ctcttggtcc agcagcgcct tggccttgct
     1561 ggcggcggcg tcagtcaggg ccgcgccgtg ggtggcggtg ctggtcgccg actcgtcctg
     1621 aacagtcatt gcgtctcctg taatggctat tgttgcgggc ctactaacag cccacctgct
     1681 caacggtacc ttgttgtgcc gctattcccg agttcgtcgg ccgtgacctc cgccaacccg
     1741 gcgagctggt ttgcggcatc cg
//
LOCUS       DASYLX010000026         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_879494,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000026 DASYLX010000000
VERSION     DASYLX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879494"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1076
                     /locus_tag="VGP27_00740"
     CDS             165..1076
                     /locus_tag="VGP27_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7849876.1"
                     /translation="MLDGQKVLITGATGKIAFPIARALAKRNEVWGAARLRKQSDRDK
                     LAAEGITPFTLDMAEPDFAELPSDFGYVFHAAVDPGADDWTRCVTANAHNSGALLHHC
                     RAAKGFVFCSTGSIYGYQGQRPLRETDPPGVPLRPNYSFSKIAAESVCTWIAGHYGIP
                     LTIIRICSTYGPQGGAPADRLDAMLAGKPIRLHPDKPNNYNPIYEDDYVDLGIRSMEV
                     AATPPVVVNWAGSETVSVESYCTYMGELVGVEPVFEYTPDAHTPLWPDVTRMHEILGR
                     TKVPWREGFRRMIGARHPEIDLPEGSS"
     gene            1073..1816
                     /locus_tag="VGP27_00745"
     CDS             1073..1816
                     /locus_tag="VGP27_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849877.1"
                     /translation="MTLPGTPSDEVEGVLAAGRGEIGTVFVSMARRHPDGCDAEYLRW
                     HTLDHRPEQHRLAGVRASLRLVSTPECRAARAYSDQRFDEIDHVMSYFFTDPSGLKGF
                     LDLSSALGGADRKLPLLPPVQRGVYAIEHKVAAPRIKIGADVLPWWPAQGAYLLLESG
                     TTESADLVGVDGVGGVWSVVSQDVDERLASAPAGQLLTYVFLDDDPVATAERLRPLLT
                     ARWERGGVEPLFAAPLYPVVPYQWDRHVP"
     gene            1826..2800
                     /locus_tag="VGP27_00750"
     CDS             1826..2800
                     /locus_tag="VGP27_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7849878.1"
                     /translation="MRVGLMVGSDKERSRADRLSGLLADGQAAEEQGFASFWVPQVPG
                     YLDAMTAVALLGHVTDRIEIGTAVVPLQTRHPLILAQQALTTQVACAGRFALGVGPSH
                     HWIITDQLGLPYDRPAHLVRDYLDVLDAAFAPGAPGAVAVDNDSFSVHSPVDVTDAFE
                     TPVLIAALGPAMLRIAGERTGGTILWMADERAIGDYVVPRITDAARAAGRSQTRVVAG
                     VPVALCSDGEIDDARAYASEVLGHADFSPNYVRLLEHGDAEDVGDTMAAGDEATVLAR
                     LRRYRDAGVTDLAVRVVPLGIGADQRSASRRRTQEFVASLTSEFAEPE"
     gene            2854..3504
                     /locus_tag="VGP27_00755"
     CDS             2854..3504
                     /locus_tag="VGP27_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7849879.1"
                     /translation="MPESSAAKVADGGQRRASFQRARSIETKRSLVQAAMALWRTNGY
                     AKTTVADICRAAGVSRALFYFYFPAKEDVLFEVGLLSTRAAQKTVHRHLAGDYDVRAV
                     INQALRSLERSMARNPRDLIVETILEGYRQEQRILTGDRPGDVDADMFGELFVRAQAD
                     GKLAGHIDVPHLSRLAQMHVSEGVRHWALGGFGDRSFSEVVASDIGALIAGFNERL"
BASE COUNT          599 a         1164 c         1176 g          610 t
ORIGIN      
        1 cggcctcggc gggcagatgc tggtcgagaa aggcgatgaa ggccgcgcgg aacgcctcga
       61 cctcgtcatc gaaccgcagt tgcacagacc caacatatca agtacttaac atttgggcgg
      121 cgatgcgcta tgactagtgg ggttgtctcg acaggagggc atcgttgctc gacggacaga
      181 aggtgctgat caccggcgca accggcaaga tcgccttccc gatcgcccgc gcgctcgcga
      241 agcgcaacga ggtgtggggc gcggcgcggc tgaggaagca gtcggaccgg gacaaactgg
      301 cggcggaggg catcaccccg ttcacgctcg acatggcgga gcccgacttc gccgaactcc
      361 caagtgattt cgggtatgtg ttccatgcgg ccgtcgaccc cggcgccgac gactggacgc
      421 ggtgtgtcac cgcgaacgcc cacaattcgg gtgcgctgct gcatcattgc cgcgccgcga
      481 agggcttcgt gttctgctcg acggggtcca tctacggtta ccaaggtcag cgcccgttgc
      541 gtgaaaccga tccgcccggc gtgccgctgc ggccgaacta cagcttctcc aagattgccg
      601 ccgagtcggt gtgcacctgg atcgccggcc attacggcat tccgttgacc atcatcagga
      661 tctgttccac ctacgggccg cagggcggcg cacctgccga ccgcctcgat gcgatgctgg
      721 cgggcaagcc gattcggctt caccccgaca agccgaacaa ctacaacccc atctacgaag
      781 acgactacgt cgacctcggc atccgctcga tggaggtggc cgccactccc ccggtcgtcg
      841 tcaactgggc aggcagcgaa accgtcagcg tcgagtccta ctgcacctac atgggcgagc
      901 tcgtcggggt cgagccggtc ttcgagtaca cgcccgacgc gcacacgccg ctgtggcccg
      961 acgtcacccg catgcacgag atcctcggca ggacgaaggt gccgtggcgc gaaggcttcc
     1021 ggcggatgat cggcgcccgt caccccgaga tcgacctgcc ggaaggctcg agttgacact
     1081 tccaggaact ccttccgacg aggtcgaggg tgtgcttgcc gcggggcgcg gcgagatcgg
     1141 caccgtgttc gtctcgatgg cgcgccgcca tcccgacgga tgcgacgccg aatacctgcg
     1201 ctggcacacc ctcgatcaca ggcccgaaca gcatcggctc gcgggcgtgc gggcgtctct
     1261 gcggctggtg tcgacgcccg agtgccgggc cgcccgcgcg tacagcgatc agcgcttcga
     1321 cgaaatcgat cacgtgatgt cctacttctt caccgatccg agcgggctga aggggttcct
     1381 cgacctgtcg tcggcactcg gcggtgcgga ccggaaactg cccctgctgc cgccggttca
     1441 gcggggtgtc tacgcgatcg agcacaaggt tgcggcgccg cgcatcaaga tcggcgccga
     1501 tgtgctgcca tggtggccgg cgcagggggc gtacctgcta ctggaatccg gcacgacaga
     1561 gtccgccgat ctcgtcggcg tcgacggagt gggcggggtc tggtcggtgg tttcgcagga
     1621 cgtcgacgaa cgcctcgcga gtgcacctgc gggccaattg ctcacatacg tcttcctcga
     1681 cgacgatccc gttgcgaccg ccgaaaggct gcggccgctg ctcaccgccc gctgggaacg
     1741 tggcggcgtc gaaccgctgt tcgccgctcc cttgtatccg gtcgtcccct atcagtggga
     1801 tcgccatgtg ccgtagagga tcgggatgcg cgtcggtttg atggtcggct ccgacaagga
     1861 gcgctcacgg gcggaccgtc tcagcggtct gctggccgac ggacaagccg ccgaggaaca
     1921 gggcttcgcg tcgttctggg tcccccaagt gcccggttac ctggatgcga tgacggccgt
     1981 tgccctactc ggccacgtca ccgaccgaat cgaaatcggc acggcggtcg taccgcttca
     2041 aacccggcac ccgctgattc tcgcgcagca ggcgttgacc acgcaggttg cttgtgcggg
     2101 ccggttcgca ctgggggtcg gcccgtcgca ccattggatc atcaccgatc agctcgggct
     2161 gccctacgac agaccggcac acttggtccg cgactatctc gacgtgctcg acgcggcgtt
     2221 cgcccccggg gcgccgggcg cggtagctgt cgacaatgac agcttttccg tgcacagccc
     2281 ggtagatgtc accgacgcat tcgagacgcc ggtgctgatc gcggctcttg ggccggcgat
     2341 gctgcggatc gccggtgagc ggacgggcgg aaccatcctg tggatggccg acgaacgggc
     2401 gatcggcgat tacgtggtgc cgcggatcac cgatgcggcc cgggcggcgg ggcggtccca
     2461 aacacgggtc gtcgcaggcg ttcccgtcgc gctctgctcg gacggtgaaa tcgatgatgc
     2521 ccgcgcgtac gcgagcgagg tgctcgggca tgctgacttc tcccccaact acgttcggct
     2581 gctcgagcac ggcgacgcag aggacgtcgg cgacaccatg gccgcgggcg acgaggcaac
     2641 cgtgctcgca cgcctgcgtc gctaccgcga cgccggtgtc accgatctcg ccgtcagggt
     2701 ggtcccactg ggcataggtg ctgaccaacg gagcgcgtcg cgtcgacgca ctcaggagtt
     2761 cgtcgcgtcc ctcacatcgg aatttgcgga gcctgagtag ttagacacgc gttaactatt
     2821 ttcatcgcct aaacgaactg ttaaagtgcg tatgtgccgg agagcagcgc cgcaaaggtg
     2881 gctgacggtg gtcagcgtcg ggcctctttt cagagggccc gctccatcga gaccaagcga
     2941 tcgttggtgc aggcggcgat ggcgctgtgg cgcacgaacg gctatgccaa gacgaccgtc
     3001 gccgatatct gccgggccgc gggcgtatcg cgcgcgctgt tctactttta cttcccggcc
     3061 aaggaagacg tgctcttcga ggtcggcctg ctgtccaccc gcgccgccca gaaaaccgtg
     3121 catcggcatt tggccggcga ctatgacgtt cgtgccgtta tcaaccaagc gctgcgcagc
     3181 ctcgagcgct cgatggcacg caacccccgc gatctgatcg tcgagacgat cctcgagggc
     3241 taccggcagg aacaacgcat cctcaccggt gatcgccccg gcgatgtcga cgccgacatg
     3301 ttcggcgagt tgttcgtcag ggcgcaggcc gacggcaagc tcgcagggca catcgatgtg
     3361 ccccacctgt cgagattggc tcagatgcac gtcagcgagg gcgtccgaca ctgggcgctc
     3421 ggcggcttcg gcgaccggtc cttttccgag gtggtggcga gcgacatcgg cgcgctgatc
     3481 gcaggattca acgaaaggct ctgacgcagt gaaggagacg cacgatggca tgggatttcg
     3541 aaaccgacc
//
LOCUS       DASYLX010000027         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1428047,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000027 DASYLX010000000
VERSION     DASYLX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428047"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGP27_00760"
     CDS             complement(<1..550)
                     /locus_tag="VGP27_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV7849880.1"
                     /translation="MNELISILYIVAFALFIYGLMGLTGPRTAVRGNIIAGVGMAIAI
                     VATLLTPGTSNWLLIALGLALGTVIGVPSAKQVKMTAMPQLVALFNGVGGGAVALVAW
                     VEFRDSHGYATVAGYVTVTSLFAAIVGSVSFWGSLIAFGKLQEVLPGRPITLGRAQQP
                     LNAALLLVSIGCAVAIGLGQRSE"
     gene            complement(543..860)
                     /locus_tag="VGP27_00765"
     CDS             complement(543..860)
                     /locus_tag="VGP27_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV7849881.1"
                     /translation="MTLLSNLAILVLAGYIGFSVISKVPNTLHTPLMSGTNAIHGIVL
                     LAGLVLLGRSASGVFERTLLVIAIAFGAINVVGGFLVTDRMLGMFKKKPASESGRVAA
                     VHE"
     gene            complement(857..1951)
                     /locus_tag="VGP27_00770"
     CDS             complement(857..1951)
                     /locus_tag="VGP27_00770"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV7849882.1"
                     /translation="MTLIGVPRENAPGERRVALVPKVVERLCGAGLSVVIEAGAGAGA
                     LIPDALYEQAGASVGDPWPADVVVKVNPPTDDEITFLKPGSIVAGFLAPRTRPEVADR
                     LRIAGVTGFAMEAVPRISRAQTMDALSSQANVAGYKAVLCAAECSTRFFPMLTTAAGT
                     VKPSTVLVLGVGVAGLQALATARRLGARTTGFDVRPEVAEQVRSLGAQWLELGVQAAG
                     DGGYARALTTEEQAEQQLRLTEAISGFDVVITTALVPGRPAPRLVTAEAVRRMRPGSV
                     VVDLAGEAGGNCELTVPGETVVRHAVTIASPLNLPATMPEHASELYARNVAALLEHML
                     ADGEIVPDLSDEILAACCVTSTTDQLTEAR"
     gene            2216..>2879
                     /locus_tag="VGP27_00775"
     CDS             2216..>2879
                     /locus_tag="VGP27_00775"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV7849883.1"
                     /translation="MKIAVVGAGAIGAYWGAAMHRGGAEVHLIARNEHLRAMRKNGVR
                     VVSPRGDFVAYPNATDDPAEIGPVDYVFLGLKAHSYPSCGPLVKPLLGENTAVLPAQN
                     GIPWWYFHQLQSPYSGRRIEAVDPSGATSAVLSPERAIGCVVYPATTIEAPGVIRHLE
                     GTRFSIGEPSGEISERCKTLSDAMIAGGLKAPVEADLRNDIWIKLMGNVAFNPLSALT
                     RAT"
BASE COUNT          533 a          931 c          956 g          459 t
ORIGIN      
        1 gctcggatcg ttggcccaga ccgatcgcga cggcacaacc gatggagacg agcagtagtg
       61 ccgcgttgag cggttgctgt gcccggccga gcgtgatcgg cctgcccggc aacacctcct
      121 gcagcttgcc gaaggcgatc aacgatcccc agaaggacac cgaaccgacg attgcggcga
      181 aaagcgaagt gaccgtgaca tatccggcca ccgtcgcgta gccgtggctg tcccggaact
      241 ccacccacgc caccagggcc accgcgccgc cgccgacgcc gttgaacagc gccaccagtt
      301 gcggcattgc ggtcatcttg acctgcttcg ccgacggcac gccgatgacc gtccccaacg
      361 cgagaccgag tgcgatcaat agccagttgc tcgtccccgg cgtcagcagg gtcgccacga
      421 tcgcaatggc catgccgacg cccgcgatta tgttgccacg caccgctgtt cgtggaccag
      481 tcaagcccat caggccgtag atgaaaagtg cgaacgcaac aatgtagaga atcgagatga
      541 gctcattcat ggacggccgc aacccttccc gattcagaag ccggcttctt cttgaacatg
      601 ccgagcatcc ggtctgtcac aaggaacccg cccaccacgt tgatggcgcc gaaggcgatg
      661 gcgatcacca gcaacgtgcg ctcgaacaca ccggacgcgg agcggccgag tagcacaagg
      721 ccggcgagca gcacgatgcc gtggatggcg ttggtcccgg acatcagcgg ggtgtgcagg
      781 gtgttgggca ccttggagat cacggagaag ccgatgtagc cggccaacac caagatcgcc
      841 agattggaga ggagtgtcat cgggcctccg tcaactggtc agtggtcgag gtcacgcagc
      901 aagcggcaag gatctcgtcg gagaggtccg gcacgatctc gccgtcggcc agcatgtgtt
      961 cgagcagcgc ggcgacgttg cgcgcgtaaa gctcgctggc gtgctccggc atcgtcgcag
     1021 gcaggttcaa cggcgaggcg atggtgacgg cgtgccgaac tacggtctcc ccgggcacgg
     1081 tgagctcgca gttgccaccc gcttcaccgg cgaggtcgac aaccacgctg cccggccgca
     1141 ttcggcgtac cgcctcggcg gtgacaagcc gaggtgccgg gcggcccgga acgagcgcag
     1201 tggtgatcac gacgtcgaaa cccgagatcg cctctgtgag ccgcagttgc tgctcggcct
     1261 gctcctcggt ggtcagcgcg cgggcgtagc cgccgtcgcc ggcggcctga acaccgagct
     1321 ccagccactg ggcgcccagc gaccgaacct gttcggcgac ctcggggcga acgtcgaagc
     1381 cggtcgtgcg ggcgcccagt cgcctggcgg tggccagcgc ctgaagcccg gcaacgccca
     1441 cgccgagtac caggaccgtg gacggcttca ccgttccggc ggccgtggtg agcatgggga
     1501 agaagcgggt cgagcattcg gcagcgcata acaccgcttt gtaaccggcg acattggctt
     1561 gagaggacag cgcgtccatg gtctgcgccc gagagatccg cggcaccgct tccatggcga
     1621 agccggtgac gcccgctatc cggagtcggt cagcgacctc ggggcgagtg cgtggtgcca
     1681 aaaatccggc cacgatcgat ccgggcttca ggaacgtgat ttcgtcgtcg gtcggtgggt
     1741 tcaccttcac cacgacgtcg gccggccagg gatcgccgac gctggcgccg gcctgttcat
     1801 agagcgcgtc ggggatgagt gccccggccc cggccccggc ctcgatcacg acggaaaggc
     1861 cggcgccgca tagtcgttcg acgaccttgg ggaccagcgc aacgcgtcgt tcgcccggtg
     1921 cgttttcccg gggcactccg ataagcgtca tattcctctt cctggctttg gccgggcaga
     1981 acgctcctgg cggttcacgt caatgtgctg atgcggcgat gcccaagccg ggagcaggcc
     2041 gaccgtagca tatcgcatac agtatccact agcgttggag cgggaccgag gcgacggggc
     2101 ttgccatctc ggcgagcacc cgatctgcat ctcgttgggc cgccaggcgt cccccaactg
     2161 ggtgatagac acccaccgcg attaatgcat acagtatgcg atcgaggagg ttgccgtgaa
     2221 gatcgcagtc gtcggtgccg gagcaatcgg cgcttattgg ggagccgcaa tgcatcgcgg
     2281 aggcgctgaa gtgcacctca tcgcgcggaa cgaacacctg cgcgcgatgc ggaaaaacgg
     2341 tgtgcgcgta gtcagtccgc gtggcgactt cgtggcatat ccgaatgcca cggacgaccc
     2401 ggccgagatc ggacccgttg attatgtttt cctcgggctg aaagcgcaca gctacccgtc
     2461 ctgcggtccc ctggtgaaac cgctgctcgg cgagaacacg gcagtgctgc ccgcgcagaa
     2521 cgggatcccg tggtggtact tccaccaatt gcaaagcccg tacagcggcc gtcggatcga
     2581 ggccgtcgat cccagcggag ccaccagtgc cgtgctgtca ccggagcggg ccatcggctg
     2641 cgtggtgtat ccggccacca cgatcgaggc gccgggagtg atccgtcacc tcgagggcac
     2701 ccggttttcc atcggtgagc cgtcggggga gatctcggag cgctgtaaga cgttgagcga
     2761 cgcgatgatt gccggcggat tgaaggcgcc ggtcgaggcc gacctgcgca acgacatctg
     2821 gatcaagctc atgggcaacg tcgcgttcaa tccgctcagc gcgctgacca gagcgacga
//
LOCUS       DASYLX010000028         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3292963,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000028 DASYLX010000000
VERSION     DASYLX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3292963"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP27_00780"
     CDS             complement(<1..351)
                     /locus_tag="VGP27_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEV7849884.1"
                     /translation="MPDAWERERPQPVAVGPPRRRMSRADRMREARRRRRHRLVRSVT
                     LCVFIVIVIGAVFLGSRLWHNVFGGGSDYSGEGRSDVVIQVHEGDSTTTIGKTLEDQK
                     VVATSRAFVNAAQDN"
     gene            complement(344..841)
                     /gene="ruvX"
                     /locus_tag="VGP27_00785"
     CDS             complement(344..841)
                     /gene="ruvX"
                     /locus_tag="VGP27_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV7849885.1"
                     /translation="MPNPENRPPDRPGGDDPGRGRRVGIDVGSVRIGVAVSDPDCILA
                     TPVETVRRDRTDRHMRRLTQLVNELDAVEVVVGLPRTLADRTGPSAEDAISLAEDLAR
                     RVSPTPVRMADERLTTVSAQRALREAGVRARGQKAMIDQAAAVGILQSWLDQRRVALN
                     EVGNA"
     gene            complement(846..>2645)
                     /locus_tag="VGP27_00790"
     CDS             complement(846..>2645)
                     /locus_tag="VGP27_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEV7849886.1"
                     /translation="SPGNDGRGYVLRRLLRRVIRSAKLLGINEPIVGDIMVTVRDAMG
                     PSYPELVNDFDRIQRIAVAEETAFNRTLASGSRLFDDAARATKQAGTAVLSGTDAFTL
                     HDTYGFPLELTLEMAAEAGLSVDEEGFRGLMAQQRQRAKADAAARKQAHTDLSAYREL
                     VDAGPTEFTGFDELTTEARILGIFVDGRRVPVVSHDSHQAERVELILDRTPFYAESGG
                     QIADEGSIAGTAASGTAKAAVTDVQKIAKTLWAHRINVESGEFVEGDTVVATVDPRWR
                     HGATQGHSGTHMVHAALRQVLGPNAVQAGSLNRPGYLRFDFNWQGSLSEDQRTQIEEV
                     TNEAVEADFEVHSFTTDLEKAKAMGAMALFGEAYPDEVRVVEIGGPFSLELCGGTHVR
                     NSAQIGPVTILGESSVGSGVRRVEAYVGLDSFRHLAKERALMAGLASSLKVPSEEVPA
                     RVAQLVDRLKSAEKEVDKMRLAGARAAAVNAAAGAERIGRVRVVAQRMAGGTSAGDLR
                     SLVGDIRGKIGSEPAVIALIAEGDNDAVPFVVAVNPAAQDLGLKANELVKALGAAVNG
                     RGGGKADLAQGSGKGVAGIDAALAALRAEIGRS"
BASE COUNT          392 a          938 c          860 g          455 t
ORIGIN      
        1 gttgtcctga gcggcgttga cgaatgcccg cgatgtcgca acaaccttct ggtcttcgag
       61 cgtcttgccg atggtcgtgg tcgagtcgcc ctcgtggact tggatgacga cgtccgacct
      121 gccttcgccg gaatagtcgg agcctccccc gaagacgttg tgccacaacc gggagccgag
      181 gaaaacggcg ccgatcacga tgacgatgaa cacgcatagc gtgacactgc ggaccaaccg
      241 atgtctgcgg cgacggcgag cttcgcgcat ccggtcggcg cggctcattc gacgccgcgg
      301 cgggcccacc gctaccggct gcggacgttc gcgctcccag gcgtcaggca ttgccgacct
      361 cgttgagtgc gacccgccgc tgatccagcc agctctgcag tattccgacc gctgcggcct
      421 ggtcgatcat cgccttctgt cccctggccc ggacaccggc ttcccgcagt gcgcgttgcg
      481 cgctgacagt ggtgagccgt tcgtcggcca tccgcaccgg agtgggcgaa acgcgacgcg
      541 ccagatcctc cgcaagagag atggcgtctt cggccgacgg accggtccga tcggcgagcg
      601 tgcgcggcag tccgaccacc acctcaacag cgtccaattc gttgacgagt tgggtcagcc
      661 gtcgcatatg gcggtccgtt ctgtcacggc ggacggtctc caccggtgtg gcgaggatgc
      721 agtcagggtc gctcaccgcg acgccgatcc gaacacttcc gacgtcgata cccactcgtc
      781 gtccccgtcc gggatcgtcg cctccaggcc gatccggcgg acggttttct gggttcggca
      841 cgtggttagc tccggcctat ctccgcgcgc agcgcggcga gcgcggcgtc gatgcctgcg
      901 actcccttgc cggagccctg agccaggtcc gccttgccgc cgccgcggcc gttcaccgcc
      961 gcgcccagcg ccttgaccag ctcgttggcc ttcagcccga ggtcctgggc ggccgggttg
     1021 acggccacca cgaacggcac ggcgtcgttg tcgccctccg caatcagcgc gatcaccgca
     1081 ggctcggagc cgatcttgcc gcggatgtcg ccgacgagac tgcgcaggtc ccctgccgat
     1141 gtgccaccgg ccatccgttg cgccacgaca cggacccgac cgatccgctc ggctcccgct
     1201 gcggcattca ctgcggctgc cctcgcaccg gccagccgca tcttgtcgac ttccttctcg
     1261 gcgctcttca accgatccac cagctgggcc acccgtgcgg gcacctcttc cgagggcacc
     1321 ttcagcgacg acgccaggcc cgccatcaat gctcgttcct tggcgagatg gcggaacgag
     1381 tcgaggccga cgtaagcctc cactcgccgc acacccgagc cgaccgacga ttcaccgagg
     1441 atcgtgacgg gaccgatctg cgcggagttg cggacgtgcg tgccgccgca cagctcgagc
     1501 gagaacgggc cgccgatctc gaccacccgc acctcgtcgg ggtaggcctc gccgaacagc
     1561 gccatcgcgc ccatcgcctt ggccttctcg aggtcggtgg tgaaactgtg cacctcgaag
     1621 tccgcctcga cggcctcgtt ggtgacctct tcgatctggg tgcgctgatc ctcggacaac
     1681 gaaccctgcc agttgaagtc gaaccgcagg tatccgggac ggttcagcga gccggcctga
     1741 acagcgttgg ggcccagcac ttgtcgcagc gcggcatgca ccatgtgggt gccggagtgg
     1801 ccctgggtgg cgccatggcg ccatcgcggg tccacggtgg cgacgaccgt gtcaccctcg
     1861 acgaactccc ctgactccac gttgatgcga tgcgcccaca gcgtcttggc gatcttctgc
     1921 acgtcggtga cggccgcctt cgcggtgccg gatgcggccg taccggcaat gctgccctcg
     1981 tcggcgatct ggccgccgga ctcggcgtag aacggcgtgc ggtcgaggat cagctcgacg
     2041 cgctcggcct ggtggctgtc gtgcgacacg accggcaccc gtctgccgtc gacgaagatt
     2101 ccgagaattc ttgcctcggt tgtcaattcg tcgaagccgg tgaactcggt ggggccggcg
     2161 tcgaccagct cgcggtaggc cgacaggtcg gtgtgcgcct gcttgcgggc agcggcgtcg
     2221 gccttcgcgc gctgccgctg ctgcgccatc aggccgcgga agccctcttc gtcgacgctc
     2281 aggccggctt cggcggccat ctcgagggtc agctcgagcg gaaagccgta cgtgtcgtgc
     2341 agggtgaacg catcggttcc ggacagcacg gcggtacccg cctgcttcgt cgcacgggcg
     2401 gcgtcgtcga agagccgtga acccgatgcc agcgtgcggt tgaacgccgt ctcctcagcc
     2461 accgcgatgc gctggatcct gtcgaagtca ttgaccagtt ccggatagga cggacccatc
     2521 gcgtcgcgca ccgtgaccat gatgtcgccg acgatcggct cgttgatgcc aagcagcttc
     2581 gcggacctga tcacgcggcg cagcaggcgg cgcagcacat agccgcgacc gtcgttgccc
     2641 gggct
//
LOCUS       DASYLX010000029         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1428138,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000029 DASYLX010000000
VERSION     DASYLX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428138"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGP27_00795"
     CDS             <1..274
                     /locus_tag="VGP27_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV7849887.1"
                     /translation="AGTDAVDMARRMGEGLVHLHLCDGSGASTDEHLVPGRGTQPTVE
                     VCEMLAGDEFSGHVILEVTTSGARTAAEREALLNESLQFAREHLLR"
     gene            335..1228
                     /locus_tag="VGP27_00800"
     CDS             335..1228
                     /locus_tag="VGP27_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7849888.1"
                     /translation="MSGSTLFTDAMALTPAGDGRYDGELNEHWTIGPRGSGPKVHGGA
                     MLALCANAARLEHSRGQERWAHARSAGDDSGEGPSVDGAWPIAISGNYLWAPDPGPMR
                     VTTTVRKRGRRVSLIDVELSQGERTAVRAAVTMGEPEHHVPPLLSVNPVVPLMTPEPP
                     PGLEPIGPGHPMAAVVNLAHGCDIRPSLTTMEPRADGGPPVIEYWVRPKGVAPDPLFA
                     LLCGDVSAPVTFGVNRFGWAPTVQLTAFLRTLPADGWLRIICTTVQIGQDWFDEDHIV
                     VDCEGHIVVQSRQLAMVPPAQ"
     gene            1258..2130
                     /gene="proC"
                     /locus_tag="VGP27_00805"
     CDS             1258..2130
                     /gene="proC"
                     /locus_tag="VGP27_00805"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV7849889.1"
                     /translation="MARIAIIGGGSIGEALLAGLLRAGRQAKDLVVAEKSADRAKYLS
                     DRYSVLVTSVADAAENASFVVVAVKPADVSAIVEEIADAAAHAETQSVEQVFVTVAAG
                     VTTEYYESKLPAGAPVIRVMPNAPVVVGGGVSALAPGRFATAEQLKEVSAIFDAVGGV
                     LTVAEAQMDAVTALSGSGPAYFFYVVEALVDAGVAAGLTRPVATDLVAQTMAGSAAML
                     LERLDVAQPAGDAALGTGMDTTAAQLRATVTSPGGTTAAGLRELEKAGVRAALAEAVE
                     AAKLRSSQLGITSG"
BASE COUNT          362 a          771 c          812 g          349 t
ORIGIN      
        1 agcgggcacc gatgcggtcg acatggcgcg ccgcatgggc gagggcctcg tgcacctgca
       61 tctctgcgac ggcagcggag cctccaccga cgagcatctg gtgcccggcc gcggcaccca
      121 gccgacggtg gaggtctgcg aaatgctcgc gggcgacgag ttctcgggcc acgtgatcct
      181 cgaggtcacc acctcgggtg cgcggacggc ggccgaacgc gaggcactgc tcaacgaatc
      241 gctgcagttc gctcgcgaac atctgctgcg ctgacccgcg gcggacaaga ggagcacctg
      301 aatctgtgcg gacgaaaacc tatgaggaga cggcatgtct gggtccacgt tgttcaccga
      361 cgcgatggcg ctcacacccg cgggcgacgg ccgttacgac ggtgagctga acgagcactg
      421 gacgatcgga cccaggggat cggggccgaa ggtgcacggc ggcgcgatgc tggcgctgtg
      481 tgcgaatgcg gcccgcctgg agcattccag ggggcaggag cgctgggccc acgcgcgcag
      541 cgcaggggac gactcggggg aaggtccgag cgtggacggc gcctggccga tcgcgatctc
      601 ggggaactac ctgtgggcac ccgatcccgg ccccatgcgc gtcacgacga ctgtccgcaa
      661 gcgcggccgc cgcgtcagcc tgatcgacgt cgagctgagc cagggcgagc gcactgcggt
      721 gcgcgccgcc gtcacgatgg gcgagccgga acatcacgtt ccgccgctgc tttcggtcaa
      781 ccccgtggtg ccgttgatga cgccggagcc gccgccgggg ctggaaccga tcggccccgg
      841 ccacccgatg gccgccgtcg tcaacctcgc ccacggctgc gacatccggc cgtcgctgac
      901 cacgatggag ccgcgcgccg acggcgggcc gcccgtcatc gagtactggg tccgccccaa
      961 gggcgtggcg cctgacccgc tgttcgcgct gctgtgcggc gatgtgtcgg ctccggtgac
     1021 cttcggcgtc aaccgattcg gctgggcgcc caccgtccag ctcacggcgt ttctgcgcac
     1081 gctgcctgcc gacgggtggc tgcgcatcat ctgcacgacg gtgcagatcg gtcaggactg
     1141 gttcgacgag gaccacatcg tcgtcgactg cgagggccac atcgtcgtcc agagccgcca
     1201 gctcgcaatg gtgccgcccg cgcagtaacc gccgcggcgg tctgcaatgc tttgaccgtg
     1261 gcaaggatcg cgatcatcgg cggtggcagt atcggcgaag cgctgctggc tgggctgctg
     1321 cgcgcaggca ggcaggccaa ggacttggtg gtggccgaga agtcagcgga ccgcgcaaag
     1381 tacctgtcgg ataggtattc ggtgctggtc acctcggtgg ccgatgccgc ggagaacgcc
     1441 tcgttcgtcg tcgtcgccgt caagcccgcc gatgtgtcgg cgatcgtgga agaaatcgcc
     1501 gacgccgccg cccacgccga gacccagagc gtggagcagg tcttcgtgac ggtggccgcg
     1561 ggcgtgacca ccgagtacta cgagtcgaag ctgcccgccg gtgcacccgt gatccgggtg
     1621 atgcccaatg cgccggtggt ggtcggcggc ggcgtgagtg cgctggcgcc cggtcggttc
     1681 gcgactgccg aacaactcaa agaggtctcg gcgatcttcg acgccgtcgg cggtgtgctg
     1741 accgtcgcgg aagctcagat ggacgccgtc acggcgctgt cgggatcggg cccggcgtac
     1801 ttcttctacg tcgtcgaggc cctggtggac gccggcgtcg cggcgggcct cacccggccc
     1861 gtggccaccg atctggttgc ccagaccatg gccggatcgg ccgcaatgct gctcgagcgg
     1921 ctcgatgtcg cccaaccggc cggggacgcc gctctgggca ccgggatgga caccacagcg
     1981 gcccagctgc gcgccacggt cacctcgccg ggcggcacaa ccgccgctgg tctgcgggaa
     2041 ctcgagaagg ccggtgtccg ggccgccctt gcggaggccg tcgaggccgc aaaactgcga
     2101 tcttcgcagc tcggaatcac atccgggtaa ttcaacgaat ttcaatcgat taccccacac
     2161 ccgtcgcaat aaccccaata accccgctat tctcctcgtg taagcgcggg gtcgtaccca
     2221 gcggtgggga agccgctgga acagcccgtg cctgaaggat tgggttgcga tgacgtctat
     2281 gaacgggcca tcgg
//
LOCUS       DASYLX010000030         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2415361,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000030 DASYLX010000000
VERSION     DASYLX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415361"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="tatC"
                     /locus_tag="VGP27_00810"
     CDS             complement(<1..624)
                     /gene="tatC"
                     /locus_tag="VGP27_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV7849890.1"
                     /translation="MQTPGFLKRMDPRRRRAKVNPDGTMSLVDHLSELRNRLLIAAAA
                     VVLTTIIGFFWYTNNFFGFHSLGEWLRGPYCSLPASARATIAPDGECRLLATAPFDQF
                     MLRLKVALTAGLILACPVWLYQVWAFITPGLYRKERRFAMAFVTVGALLFVTGAVLAY
                     VVMAKALHFLLTVGSDVQVTALSGEQYFGFLINLLLVFGISFEFPLLI"
     gene            complement(695..970)
                     /gene="tatA"
                     /locus_tag="VGP27_00815"
     CDS             complement(695..970)
                     /gene="tatA"
                     /locus_tag="VGP27_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEV7849891.1"
                     /translation="MGGLQPWHWVIVIAVFVLLFGAKKLPDAARSLGKSMRIFKSEIK
                     EMQSDTKPETEKPATAIASERVETPEARSDVPGQAAPAAEPTDKRPA"
     gene            complement(1001..1972)
                     /locus_tag="VGP27_00820"
     CDS             complement(1001..1972)
                     /locus_tag="VGP27_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV7849892.1"
                     /translation="MSPVSERLVRLLNMVPYFQANPRITHAEAASDLGVTEKQLRDDL
                     NQLWMCGLPGYGPGDLIDFEFSGDTIEVTFSAGIDHPLRLTSPEATGVLVALRALVDV
                     PGMVDPEAARSAIAKIESAAGTVSHGAEAAVDEPAPVESQAAAAVREAVRGNRALSIE
                     YYSASHDMLTSRIVDPIRVVLVAEHSYLEAWCRSAEGVRLFRFDRIVDASVLDEPSAP
                     PEPAVQSPPDTSLFDADPALPSATLLIDRSASWMFDYYPLQVLRELPGGACEASMTYA
                     SDDWMARFVLGFGSGVRVLAPELLAQRVRESASAALRAYGVGAESGG"
     gene            complement(2070..>3017)
                     /locus_tag="VGP27_00825"
     CDS             complement(2070..>3017)
                     /locus_tag="VGP27_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV7849893.1"
                     /translation="LLSTRTYISAERIRETVYGYSDSASDEAFSRMFERDKNELRDLG
                     IPLETGRVSQFDPTEGYRINRDAYALPAVDLTADEAAAVAVATQLWESPELITATQGA
                     LLKLRAAGVDVDAGDAGIAITPTAGMPGIRGSEEVLGILLSAINSGQAVQFPHRPARS
                     EPYTTRTVEPWGVVTDRGRWYLVGHDRDRNATRTFRLSRIGADVKAIGPAGAVTPPDR
                     VNLREIVHKVIGETPTGTQARVWVADGRATALRRLALVVGPRELDGRDGEEISIDIGM
                     SDRLAREIASYGADAVVLEPQALRDEVLERLRAQAEVGV"
BASE COUNT          501 a         1043 c         1001 g          472 t
ORIGIN      
        1 gatcagcagc gggaactcga aactgatgcc gaacaccaga agcaggttga tcaagaagcc
       61 gaagtactgc tcgcccgaca gcgcggtgac ctgaacgtcg ctgccgacgg tcaaaaggaa
      121 gtgcagcgct ttggccatca cgacgtaggc gagcaccgcg ccggtcacga acagcagtgc
      181 cccgacggtc acgaacgcca tcgcaaagcg gcgttccttc cggtacaggc caggggtgat
      241 gaacgcccac acctggtaca gccagaccgg gcaggccagg atgagccccg cggtcaacgc
      301 gaccttgagc cgcagcatga actggtcgaa cggagccgtg gccaggagcc ggcactcgcc
      361 atcgggtgca atggtggccc gcgcggaggc gggcagcgaa caatacggcc cgcgaagcca
      421 ctcaccgagg ctgtgaaagc cgaagaagtt gttcgtgtac cagaaaaacc cgatgatcgt
      481 cgtcagcacg acagcggccg cggcgatcag cagtcggttg cgcagttcgc tcagatggtc
      541 gacgagcgac atcgtcccgt ccggattgac cttcgcgcgc cgtcggcgcg gatccattcg
      601 cttgaggaac ccgggggttt gcacggaacc tgcctataaa cctatgaaca ccgagaatcg
      661 acgcggcctg ctgccgcggt cggtcggcgt gggctcaggc cgggcgcttg tcggtcggct
      721 cggccgccgg agccgcctgg ccggggacat cggatcgagc ctcgggtgtc tcgacacgct
      781 ccgaggcgat cgcagtcgca ggcttttcgg tctccggctt ggtatccgac tgcatctcct
      841 tgatctccga cttgaagatc cgcatcgact tacccagcga acgggccgca tccggaagct
      901 tcttggcgcc gaaaagcagt acgaagacag cgataacgat cacccagtgc cagggttgta
      961 aaccgcccaa ttttgttacc tccagacgtc tgaggcgagt ctacccgccc gactctgcgc
     1021 cgaccccata cgcacgaaga gccgccgagg ccgattcgcg aacccgttga gcgagcaatt
     1081 cgggtgccaa cacccgcacc ccagagccga agcccagcac gaagcgggcc atccagtcgt
     1141 cggaggcgta cgtcatggac gcctcgcacg ccccgccggg aagctcccgg agcacctgca
     1201 gcggatagta gtcgaacatc caggacgccg accggtcgat cagcagcgtc gccgacggca
     1261 gcgccggatc cgcgtcgaac aacgaggtgt ccggcgggct ttgcaccgcc ggctcgggcg
     1321 gcgccgacgg ctcgtcgagc acggatgcgt cgacgatgcg gtcgaaccgg aacagccgca
     1381 cgccctcggc ggaccggcac caggcctcca ggtagctgtg ctcggcgacc agcacgacgc
     1441 ggatcggatc gacaattctg ctggtcagca tgtcgtggga ggccgagtaa tactcgatgg
     1501 acagcgcccg gttgccccgc accgcctcgc gtaccgcggc cgccgcctgg ctttccaccg
     1561 gcgcgggctc gtcgaccgcg gcctcggcac cgtgggagac cgtgcccgcg gcggattcga
     1621 tcttcgcgat cgcgctgcgt gcggcctccg ggtcgaccat ccccggcacg tcgacgaggg
     1681 cccgcagcgc caccagcaca ccggtggctt ccggcgaggt cagccgcagg gggtggtcga
     1741 tgcccgccga gaaggtcacc tcgatggtgt cgccggagaa ctcgaagtcg atcaggtcgc
     1801 cggggccgta gcccggcagg ccgcacatcc acagctggtt gaggtcgtca cgcagttgct
     1861 tctcggtcac gccgagatcc gatgcggcct cggcgtgggt gatcctcggg ttggcctgga
     1921 agtacggcac catgttcagc agccggacca ggcgctcgga gacggggctc atgcctcgac
     1981 tcccgctcgt cccctgccct gcgggcgtgg gcccaccggt cctcgctcgt tcctcgctgc
     2041 gatcctcact cgcgctcgtc ttcgggtgct catacgccca cctccgcctg ggcgcgcagc
     2101 cgctccagca cctcgtcgcg cagcgcctgc ggctcgagca ccacggcgtc ggcgccgtag
     2161 ctggcgatct cgcgcgccag ccggtcggac atcccgatgt cgatgctgat ctcctcgccg
     2221 tcgcggccgt cgagctcccg tggaccgacc accagtgcga gcctgcgcag cgcggtggcg
     2281 cggccgtcgg cgacccacac cctggcctgc gttccggtcg gcgtctcgcc gatgaccttg
     2341 tgcacgatct cgcgcaggtt caccctgtcg ggcggcgtga ccgcaccggc cggaccgatg
     2401 gccttcacat ccgccccgat ccgggacagc cggaaggttc gggtggcgtt acggtcacgg
     2461 tcgtgtccga cgaggtacca gcgtccgcga tcggtgacca cgccccaggg ttcgaccgtg
     2521 cgcgtcgtgt agggctcact gcgcgcgggc cggtgcggga actgcacggc ctgaccggaa
     2581 ttgatggccg acagcaaaat tcccagcact tcttcggatc cgcggatacc gggcattccc
     2641 gccgtcgggg tgatggcgat gcccgcgtct ccggcgtcga cgtccacgcc tgcggccctc
     2701 agcttgagca gtgccccctg cgtggcggtg atcagctccg gcgactccca cagctgggtg
     2761 gccacggcga cggcggccgc ttcgtcggcg gtcaaatcga cggccggcag cgcgtacgca
     2821 tcgcggttga tgcggtaacc ctcggtgggg tcgaactgcg agacgcggcc tgtttccaga
     2881 ggaatgccca ggtcgcgcag ttcgttcttg tcgcgttcga acatccgcga gaacgcctcg
     2941 tcgctggcgc tgtccgagta gccgtagacc gtctcccgga tccgctctgc actgatgtag
     3001 gtgcgggtgg acagcag
//
LOCUS       DASYLX010000031         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_440837,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000031 DASYLX010000000
VERSION     DASYLX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440837"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1894
                     /locus_tag="VGP27_00830"
                     /pseudo
     CDS             200..1894
                     /locus_tag="VGP27_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856390.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          350 a          627 c          629 g          288 t
ORIGIN      
        1 gaccatccgc gacgccgacc agatcctggt gatggaggac ggcaggatcg tcgagcgcgg
       61 gagccacgcc gaactgctgg cgcgccgcgg cgaatactgg tcgatggccc aggcctgacc
      121 tccgcgcgga gaaacgcacg gagaaaagag cgatgcgctt aacggcgccc acggttgagc
      181 gggtcgggca ggatgcccca tgagcaccaa cacggtgcac acgttctgcc ggtactgcct
      241 ggcgtcgtgc ggcatcgagg tcacggtcga ggacaaccgg gtcaccaaga tctcggctga
      301 caagcagaat ccccacacct ggcgggactt ctgcgccaag ggccgcaccg ccaaccaact
      361 cgtcgagcat ccgcgccgca tcctgaaccc gatgcgtcgc gtcggcgaca cgtatgtcga
      421 ggccacgtgg gacgacgcca tcgccgacat cgcggcgcga atgaacgcgg tgatcgacac
      481 cggcgggccc gacgcggtcg gcgcctacta cggcaatccc gcgggcttct cgtcgtcgac
      541 atcatcttct acaacgcttt gctggacgcg atcggtacgc ataaccggta ctccgtcggc
      601 tccgtggatc aaaacgcgat gcatgtggtg gcctccgcga tgtacggctc tatgctgatg
      661 gccccggtgt ccgacatcga caactgcgat tacttcctcc tcgtcggcac caatcccgct
      721 gtcagtgcat ggaattggct ggagacggtg cccggcggat ggaagcgggc actcgagcgg
      781 cgacggcagg gcgcgaagat cgtcgtcgtc gatcccctgc acaccgagac cgccgagaag
      841 gccgacgtgc acctcgccgt tcggccagga caggactggg cgctgctgtt ggcgatggtc
      901 aaagtggtgc tcgaggaggg cttggagcac aaacaggact gcaccgacct cgccaccggc
      961 gtcggcgaac tgcgaacgct cgtcgccgac gccgatctcg acgacctcgc gctgcgttgc
     1021 gatgtccccc gcgcggaaat cgagcagact gcacgagatt tcgccgccgc ccgaggtgcg
     1081 atggtggtga cccgcaccgg cgtctcgatg catctcgccg gaactgtcgc cgagtggctc
     1141 gggcacgtgc tcaacgtcat caccggccgg atggaccgcc caggcggccg gcgctacgag
     1201 ccgggctacg tcgacgccgt cgggctgtcc gcaatgatca aggcgaagcc gcactccagc
     1261 cggctgcggg gccgtgaaat ggtcgcgggc gcacacgcac tcagcgaatt gcccgacgag
     1321 ataaccactg ccgggcccgg ccagatccgt gcgatggtgc tcaactgcgg caacccggtg
     1381 gtgtccgggc ccgacggcgc gaagctcgac ggtgcattcg aacagctgga cctgctggtg
     1441 gccatcgact tcgtgcagcg cgaaagccat cggcatgcgc actggctgct gcccgctgtg
     1501 cactggctgg aacgcgacga cctcctcgcg ttcaccagca acatgcatga cgagccgtat
     1561 ctgcagttcg gggcgaaggc cgtcgatccc ccaccgggcg cgcgacagga gtggcgaatc
     1621 ttcaccgacc tcgccatcgc catgaagaag ccgctgttca aggcgcgagg gctgaacggc
     1681 ttcatcgtcg gaacccggcg ggcagcccgc ctcacccgaa tgtcgggcct ggaattcggg
     1741 ccgcactgga tcgaccggct gttggtggcc accagtcgaa agatcaacgg ccgcagggtc
     1801 aaatggcgtg acgtcttggc gcacccgcac ggctgggtgt tgggtccgcg ggaattcggg
     1861 cacttcaggg acgcattgcg aacagacgac aaga
//
LOCUS       DASYLX010000032         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_111734,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000032 DASYLX010000000
VERSION     DASYLX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111734"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGP27_00835"
     CDS             <1..451
                     /locus_tag="VGP27_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV7849894.1"
                     /translation="QEVDNALNQAAKELSTRFDFRGTDTRIEWQGEEAITITSSTEER
                     VKAAVDVFKEKLIRRDISMKAFDAGEPQASGKTYKVSGSIKQGIDSENAKKITKLIRD
                     EGPKGVKAQIQGDEVRVSSKKRDDLQAVIALLKNADLEVAVQFVNYR"
     gene            complement(448..924)
                     /locus_tag="VGP27_00840"
     CDS             complement(448..924)
                     /locus_tag="VGP27_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849895.1"
                     /translation="MRPHHEESSTPPPPAAVETFSDADTVKQVVDPGKAIVKVAQLQE
                     VSGGVGFEACNDQGEPPYRGRLEMGFRIPDDTDPKKYFEQIAQTMVQQGVGWYDGPPP
                     GKNPFGTVIHTDTVFAVIGPNPVAREDGYVHVFGECRNMSEHRDGSSRNVTDQLLS"
     gene            complement(983..>2083)
                     /locus_tag="VGP27_00845"
     CDS             complement(983..>2083)
                     /locus_tag="VGP27_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7849896.1"
                     /translation="VLADPAFYGLSTNDVTRYHNALKVRQGFNHQRGDDPDPSRQRPV
                     MLWRYEPLADTGEGRAAIAIGNPDYAEHTTVIVPGTGSSVNGGWLSDGHNDAMNLWDQ
                     ANAADPDSTHSVISWMGYDAPDGFTDTRVANPDLARAGGDLLAADVNGLWTTHQDPQQ
                     HMTVIGHSYGSTTVADAFAHSGMHANDAVLLGCPGTDVARSVADFHLDGGHVYVGSAS
                     TDPVGYIGTAPEYLHDYLNRKLGYPVGLDGGLGLDPAGDQFGSIRFDAEVAGRDGLDA
                     GDHSHYYDMGSESLRAMTYIATGESGELGDKGLLADGRHQPHVDLPTEIDLPFGGKID
                     LPPVGFDIPGSPAYLDPEARRPAESVTKDHAF"
BASE COUNT          352 a          677 c          698 g          356 t
ORIGIN      
        1 ccaagaagtc gacaacgcac tgaaccaggc cgccaaggag ctgtccaccc gcttcgactt
       61 ccgcggcacc gacacccgca tcgaatggca gggcgaggaa gcgatcacga tcacctcgtc
      121 caccgaggaa cgggtcaagg cggccgtcga cgtcttcaag gaaaagctga tccgccgtga
      181 catctcgatg aaggccttcg acgcgggcga gccgcaggcc tcaggcaaga cgtacaaggt
      241 cagcggcagc atcaagcagg gcatcgacag cgagaacgcg aagaagatca ccaagctgat
      301 ccgggatgag ggccccaagg gcgtcaaggc tcagatccag ggcgacgagg ttcgcgtcag
      361 ctccaagaaa cgtgacgacc tccaggccgt catcgccctg ttgaagaacg ccgacctcga
      421 ggtcgcggtg cagttcgtca actaccgcta gctcagcagc tggtcggtga cgttgcggct
      481 ggaaccgtcc cggtgctcgg acatgttgcg gcactcaccg aacacgtgca cgtagccgtc
      541 ttcgcgcgcg accggattcg ggccgatgac cgcgaacacc gtgtcggtgt ggatgaccgt
      601 tccgaacgga ttcttgcccg gcggcggacc gtcgtaccag cccacacctt gctgcaccat
      661 cgtctgcgcg atctgctcga agtacttctt cgggtccgtg tcgtccggaa tccggaagcc
      721 catctcgagc ctgccgcgat acggcggctc accctggtcg ttgcaggcct cgaatccgac
      781 gccgccgctg acctcctgta gctgcgcgac cttcacgatc gccttgcccg ggtcgaccac
      841 ctgcttgacg gtgtccgcat ccgaaaacgt ctccactgct gcgggtggtg gcggcgtgga
      901 ggactcctcg tggtggggcc gcatcgtgca ccctcctaac acgacggcga gcccgaggat
      961 cgccgcacag gatcgaaaag gtctagaagg catggtcttt ggtcaccgac tccgctggac
     1021 gacgggcttc gggatcgagg tacgcaggcg aacccggtat atcgaagcct acgggcggca
     1081 aatcgatctt tccgccgaac ggcaggtcga tctcggtggg caggtcgacg tgcggctgat
     1141 gacggccatc ggcgagcagt cctttgtcgc caagctcgcc gctttcgccg gtggcgatgt
     1201 acgtcatcgc gcgcagcgac tcgctgccca tgtcgtagta gtgcgagtgg tctcctgcgt
     1261 cgaggccgtc gcgcccggcg acctcggcgt cgaaccggat cgaaccgaac tggtcgccgg
     1321 cgggatcgag tccaaggccc ccgtcgagcc ccaccggata gcccagcttc cggttcaggt
     1381 agtcgtgcag gtactccggt gcggtgccga tgtatccgac cggatccgtc gacgcggagc
     1441 cgacgtagac gtgtccgccg tcgaggtgga agtcggcgac gcttctggcc acatccgttc
     1501 ccggacaacc caacaggacc gcgtcgttgg cgtgcatgcc ggagtgcgcg aacgcgtccg
     1561 ccaccgtcgt cgacccgtag gagtgcccga tcacggtcat gtgctgttgg ggatcctggt
     1621 gggtggtcca gagcccgttg acatcggctg ccaacaggtc gccgccggca cgcgccagat
     1681 ccggattggc gacgcgcgtg tcggtgaagc cgtccggtgc gtcgtagccc atccaggaga
     1741 tcaccgagtg ggtgctgtcc gggtcggccg cgttggcctg gtcccagagg ttcatggcgt
     1801 cgttgtggcc gtcgctgagc cagccgccgt tcacgctgct gcccgtgccg ggcacgatga
     1861 ccgtggtgtg ctcggcgtag tccgggttac cgatcgcgat cgcggcccgg ccctcgccgg
     1921 tgtcggcgag cggctcgtag cgccacaaca tgacgggccg ctgccgcgat gggtcggggt
     1981 cgtcgccgcg ctggtggttg aaaccctgcc ggaccttcag cgcgttgtgg tagcgggtga
     2041 cgtcgttcgt cgagagaccg tagaaggcgg ggtcggccag cac
//
LOCUS       DASYLX010000033         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1428244,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000033 DASYLX010000000
VERSION     DASYLX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428244"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..410
                     /locus_tag="VGP27_00850"
     CDS             129..410
                     /locus_tag="VGP27_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEV7849897.1"
                     /translation="MLADLAGVIVLALVVVAFAVAVYAFVHAAMQRPDAYTAAGKLTK
                     PVWLLIIGGSVLLLLVFRDPFGAAIAAVASGIYLVDVRPKILDIQGKSR"
     gene            415..825
                     /locus_tag="VGP27_00855"
     CDS             415..825
                     /locus_tag="VGP27_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HEV7849898.1"
                     /translation="MRRVLAACAAALTAALTLAGTATADPVPGPPYVAHTEWAKWGDL
                     SSLRVYPTDSARIASIEVGTTAAADEAWTEVLTSSPDADIPGMREQFMCHWQYAELAY
                     PGKTSWNLEPWRPEVSSDQMLAAHCNPGGTEEPF"
     gene            825..1520
                     /gene="deoC"
                     /locus_tag="VGP27_00860"
     CDS             825..1520
                     /gene="deoC"
                     /locus_tag="VGP27_00860"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV7849899.1"
                     /translation="MPQTRSRGEVAALVDHTLLKPESTQADVTALVAQAADLGVYAVC
                     VSPSMIATAKSSAPAGLHIASVVGFPSGKHVSAIKAAEAAQAVNDGADEIDMVVDVGA
                     AASGEFDSVRADVAAVRAAVRDAVLKVIVESAALLRLADDNTLASVCRAAVEAGADFV
                     KTSTGFHPSGGASVHAVALMAATVGPRIGVKASGGIRTADDAVAMLNAGATRLGLSGT
                     RAVLDGLDGLTAT"
     gene            complement(1647..>2124)
                     /locus_tag="VGP27_00865"
     CDS             complement(1647..>2124)
                     /locus_tag="VGP27_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HEV7849900.1"
                     /translation="KGMKAQVNIDDVRLQDNGNSKGTIGALNATITWTADGIKQTLQD
                     SIPVVGSFITDVKTNPSDGTIELAATLGSITTKPAVQDGALALQVVNLSGLGFTLPRE
                     TVQPALDAFSTQLTKNYPLGIKADDVQVTDTGVTAHFSTKNASIPAGNTDPCFAGL"
BASE COUNT          334 a          702 c          709 g          379 t
ORIGIN      
        1 gccgccaaga aggcgccggc caagaaggtt ccggccaaga aggccccggc caagaaggct
       61 ccggccaaga aggtcaccca gaagtaaatt cgacgccgac ggtggcgccc gcttaggctg
      121 gtgaggtgat ccttgccgac ctagcgggcg tcattgtttt ggccctcgtc gttgttgcat
      181 tcgccgtggc cgtctacgcc ttcgtccacg cagccatgca acgccccgac gcgtacaccg
      241 cggcaggcaa gctcaccaag ccggtttggc ttctgatcat cggcggcagc gtgcttctgc
      301 tgctggtgtt ccgggatccc ttcggtgcgg cgatcgccgc cgtggcgtcg ggcatctatc
      361 tggtcgacgt ccggcccaaa atcctcgata tacagggaaa gtcgcggtag ccgcgtgcgc
      421 cgtgtcctcg ccgcctgcgc ggcggccctg actgctgccc tcacgctcgc aggcaccgct
      481 accgccgatc ccgtcccggg tccgccgtac gtcgcccaca ccgaatgggc caagtggggc
      541 gacctgtcca gcctgcgtgt ctatccgacc gactccgccc gcattgcgtc catcgaggtc
      601 ggcaccaccg ctgcggccga cgaggcgtgg accgaagtgc tgacgtcttc gccggacgcc
      661 gatatcccgg gcatgcgtga acaattcatg tgccactggc agtacgcgga gctcgcgtac
      721 ccgggcaaga ccagttggaa tctcgagccg tggcggccgg aggtgtccag cgaccagatg
      781 ctcgcggcgc actgcaaccc cggcggcaca gaagaaccgt tctagtgccg cagacgcgca
      841 gccgcggcga agtcgccgcg ctcgtcgacc acacactgct caagcccgaa tcgacacaag
      901 cggatgtgac ggcgttggtg gcgcaggccg ccgacctcgg ggtgtatgcg gtgtgcgtgt
      961 cgccttcgat gatcgcgacc gcgaaatcgt cggcgcccgc gggtttacac atcgcttcgg
     1021 tggtgggatt cccgtcgggc aaacacgttt cggcgatcaa ggccgccgag gcggcgcagg
     1081 ccgtcaatga cggcgccgac gagatcgaca tggtcgtcga cgtgggcgcc gccgcatcgg
     1141 gtgaattcga cagcgtccga gccgatgtgg ccgcggtccg tgccgctgtt cgcgacgccg
     1201 tgctcaaggt gatcgtggag tcggcggcgc tgctgaggct ggcagacgac aacaccctgg
     1261 cgtcggtgtg ccgggccgcg gtcgaagcgg gcgccgattt cgtgaagacc tccaccggct
     1321 ttcatcccag tggcggggca tcggtgcacg cggtcgcact tatggccgcc accgtcggtc
     1381 cccgaatcgg ggtcaaggcc agcggcggta tccgcacggc cgacgacgcc gtcgcgatgc
     1441 tgaatgcggg cgcgacccgg ctcgggcttt ccggaacccg cgctgtgctc gacgggctcg
     1501 acgggcttac cgcgacatag ctcgccgcga catagccagc cgcgacattg ccaggcgcga
     1561 cattgcccgc cgcgacattg cccgccgaga tcgacgaaat ggcggcggtt actcctactt
     1621 ttccgcccaa tcgttggttt gggcgatcag agaccggcga agcaggggtc cgtgttgccc
     1681 gcggggatcg acgcgttctt cgtcgaaaag tgtgctgtca caccggtatc ggtgacctga
     1741 acatcgtcgg ccttgatacc gagcgggtaa ttcttggtca gctgcgtgga gaacgcgtcg
     1801 agcgcaggct gcaccgtctc ccgtggcagc gtaaagccga gcccgctgag gttgaccacc
     1861 tgcagcgcga gcgcgccgtc ctgcaccgcc ggcttggtgg tgatgctgcc cagtgtcgcc
     1921 gcgagttcga tggtgccgtc ggacgggttg gtcttgacgt ccgtgatgaa gcttccgacg
     1981 acgggaatgg aatcctgcag ggtctgcttg atgccgtcgg cggtccaggt gatcgttgcg
     2041 ttcagcgcgc cgatggtgcc cttcgaattc ccgttgtcct gcaaccgcac atcgtcgatg
     2101 ttcacctgcg ccttcatgcc cttg
//
LOCUS       DASYLX010000034         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_879809,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000034 DASYLX010000000
VERSION     DASYLX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879809"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGP27_00870"
     CDS             <1..193
                     /locus_tag="VGP27_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV7849901.1"
                     /translation="RLLSFASPLHLYAEELEPRTGRHLGNFPQAFTHLALINAVVHVI
                     RAEEDADGSGAFQPANAPT"
     gene            351..566
                     /locus_tag="VGP27_00875"
     CDS             351..566
                     /locus_tag="VGP27_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7849902.1"
                     /translation="MRIVVDLNRCLGYAQCVPLAPKVLQLQGEEALTYDPNPDDSQRQ
                     QVARAVASCPVQAIIAEMDPPADRTTK"
     gene            563..1966
                     /locus_tag="VGP27_00880"
     CDS             563..1966
                     /locus_tag="VGP27_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7849903.1"
                     /translation="MSTHPLITDLVNTFKAEGRIVIVGASLAGLRAAEALRDEGFTGS
                     LTLIGDEIHEPYDRPPLSKQVLKGWVPAGNTKLPRMRAIDADWRLGVAATGLDRDNRE
                     VLLANGDKVPYDRLLIATGVRSRAWFNRDEAKLKGLYTLRTSDDAAELQAALKAGPKR
                     VLIVGAGFIGSEMASVCRDLGLDVTVAERSSAPLVGPLGGVIGDIAAKMQRDAGVDLR
                     TGVAVEALKGDADGHVRQAQLSDGTLLDVDVVVASLGSVRNVEWLEGAELATGFWGLA
                     CDAGCRAFDINGVVTDNIFVAGDIARAPHVLYEYQFMAIEHVDAAVLGAQTAAHNMVH
                     LERGRRPHLPLPQFWSGQFGINIKGVGVCSFGDEIVFTQGSVEDLRFAAAFGKKGRIV
                     GAITFNHGKWLEYYGRLIERSAPFPPPPPGYDVPVNAEPMSARFPDPREPTGIPDVVL
                     TGHDPTSRGAEFRPKAR"
     gene            1989..3263
                     /locus_tag="VGP27_00885"
     CDS             1989..3263
                     /locus_tag="VGP27_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7849904.1"
                     /translation="MTTAETAWTEAMKYENRHDPYLFFDELRKTPVVQVADKVYVVTG
                     YRELLQLAHDPHVSSDISKSPISAQPAADAPDVGAEHMQAYGREPSLIVSDPAKHDKQ
                     RRQVMRHFAPPHSPNVIPDMAADIQTLCNDSLDKIKANMGNTFDVVDDYAYPVPVAVI
                     CQILGVPLKDEPTFHGWIFDFMAGADMGPDAATEEGLARKQKGKESTAALTAYMADLI
                     QGFLKQPGDNVLSKLVNDKDGPDGPMTPKEAAANAMLLLIAGHDSTVNTIAHCVLTLL
                     RNPESIDLLHENPELIPKAIEEVLRLQSAVQFFPSRSVTADVEVGGVVIPAGSAVHLL
                     YGAANRDPNRFPDPEKFDIQRPDNQHVGWGRGVHSCVGGPLARLEVNIALETFLRRVE
                     NPRLVVDPPPYRVNQVFRGPLHLEVEFDRIAD"
     gene            3265..4305
                     /locus_tag="VGP27_00890"
     CDS             3265..4305
                     /locus_tag="VGP27_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7849905.1"
                     /translation="MKAFQFVEWQQPGQLRDVPVPEPGPGEVLVKVGGAGACHSDLHL
                     LEAPAGSKSMTLPFTLGHENAGWVETMGPGATGFALGDPVIVYGPWGCGLCMNCRQGM
                     ENYCQTPGKPSPGGLGGIDGGMAEYLVVPATRYLIPLGDLDPREAAPLTDAGLTSYHA
                     VKRSAHLLGPGSTAVVIGAGGLGQMAIQVLKALASATTVVAVDTSADKLEIAKTVGAD
                     EVLVSGDDAIKTIQDITKGQGAELVLDLVGVDPTLSMAAAVSRVLGHLTIVGLGNAAL
                     PVNFNSPAKECSVASPFWGTIPELIEVISLAQTGKIKMLVEHFALDDAAHAYHLLHEG
                     KIQGRAVITPND"
     gene            complement(4768..5152)
                     /locus_tag="VGP27_00895"
                     /pseudo
     CDS             complement(4768..5152)
                     /locus_tag="VGP27_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            5424..>5590
                     /locus_tag="VGP27_00900"
     CDS             5424..>5590
                     /locus_tag="VGP27_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HEV7849906.1"
                     /translation="MTTAQETKPAAGRYELSHLRALEAEAIHIIREVAAEFERPVLLF
                     SGGKDSIVMLHL"
BASE COUNT         1041 a         1799 c         1810 g          940 t
ORIGIN      
        1 gcgtctgctg tcctttgcga gcccgctgca cctgtacgcc gaggagctcg agccgcgcac
       61 cggtcggcat ctcggcaact tcccgcaggc gttcacccat ctggcgctga tcaacgccgt
      121 ggtgcacgtc atccgcgccg aagaggacgc agacgggtcc ggagcgttcc agcccgcgaa
      181 tgctccgacc tgacggcgtg gccggccaag gaggtgatct tgatttgcgg cgcggcaaca
      241 tcaaaaaggt aatttacgac tgcgtaagcg gtactgtcgg ctcccttccc gcggcaatac
      301 gggagcagcg ctgaccagcc ctcagcgccg gttgagagga gccgaaaagc gtgcgcattg
      361 tggtggattt gaaccgatgc ctggggtacg cgcaatgcgt cccgctcgca ccaaaagtgc
      421 ttcagttgca gggcgaggag gcgctcacgt acgacccgaa ccctgatgac tcgcaacgtc
      481 agcaggtcgc gcgggcggtg gcgtcctgcc cggtgcaggc gattatcgcg gagatggacc
      541 caccagcgga caggacgacg aagtgagcac ccaccccctg atcaccgacc tcgtcaacac
      601 cttcaaggcg gagggtcgga tcgtcatcgt cggtgcatcg ctcgccgggc tgcgtgccgc
      661 cgaggcgttg cgggatgagg gcttcaccgg gtcgttgacg ttgatcggtg acgaaatcca
      721 cgagccgtac gatcgtccac cgctgtctaa gcaggtactc aagggatggg taccggcggg
      781 gaacaccaag ctgccacgga tgcgcgcgat cgacgccgac tggcgccttg gcgtggcggc
      841 gacaggtctg gatcgtgaca accgcgaggt gttgctggcc aacggcgaca aggtgccgta
      901 cgaccgattg ctgattgcga ccggcgtccg ttcccgcgcc tggttcaacc gcgacgaggc
      961 caagctgaag ggcctctaca cgctccggac cagcgatgac gcggccgagt tgcaggcggc
     1021 gttgaaggct gggccgaagc gggtgttgat cgtcggcgcg ggcttcatcg gatccgagat
     1081 ggcctcggtc tgtcgtgatc tcggattaga cgtcacggtc gcggaacgct ccagcgcacc
     1141 cctggtcggg cccctcggcg gggtgatcgg cgatatagcc gccaagatgc agcgcgacgc
     1201 cggagtcgac ctgcgcaccg gggtggcggt ggaagcgctg aaaggcgacg ccgacgggca
     1261 cgtccggcag gctcagttat ccgacgggac cctgctcgac gttgacgtgg tggtggcgtc
     1321 gctggggtcg gttcgcaatg tcgaatggct cgaaggcgcc gaactggcca ccgggttctg
     1381 ggggctggcc tgcgacgccg gctgccgcgc tttcgacatc aacggcgtcg taaccgacaa
     1441 catcttcgtc gcaggcgaca tcgcgcgtgc cccacatgtg ctctacgagt accaattcat
     1501 ggcgatcgaa cacgtggacg cagcggtact cggcgcccag accgcggcgc acaacatggt
     1561 tcaccttgag cggggaaggc gtccgcacct accgctgccc caattctggt ccggccagtt
     1621 cgggatcaac atcaagggcg taggcgtgtg ctcgttcggt gatgagatcg tcttcaccca
     1681 gggttcggtc gaggatcttc gtttcgctgc cgccttcggc aagaagggtc gcatcgtggg
     1741 agcgattacc ttcaaccacg gcaagtggct ggagtactac ggcaggctca tcgagcgatc
     1801 ggctcccttc ccgccgccgc ccccgggcta cgacgtaccc gtgaacgccg agccgatgtc
     1861 ggcccggttc ccggatccac gtgaaccgac cgggattccc gacgtcgttc tcaccggcca
     1921 cgatccgacc tcacggggcg ccgagttccg ccccaaagcc cgctagcccg ccccaggaaa
     1981 gaagcgccat gacaaccgct gaaaccgcct ggaccgaggc gatgaaatac gagaaccgcc
     2041 acgaccctta tctgttcttc gacgaactcc gcaagacgcc cgttgtgcag gtagccgaca
     2101 aggtctacgt ggtcaccggc taccgcgaac ttttgcagct cgcgcatgac ccgcacgtga
     2161 gctccgacat ctcgaagagt cccatctcag ctcagcccgc agccgacgcg ccggacgtcg
     2221 gcgcggaaca catgcaggcc tacggtcgcg agccgagcct gatcgtgtcc gaccccgcca
     2281 agcacgacaa gcagcgcagg caggtcatgc ggcacttcgc tccaccgcac tcgccgaacg
     2341 tcatccctga catggcagcc gatatccaga cgctgtgcaa tgactcactg gacaagatca
     2401 aagccaacat gggcaacacc ttcgacgtcg tcgacgacta tgcctatccg gttccggtcg
     2461 cggttatctg ccagatcctc ggggtcccgt tgaaagacga acccaccttc cacggctgga
     2521 tcttcgattt catggccggc gcggacatgg ggccggacgc cgctaccgaa gaaggactgg
     2581 cccgcaagca gaagggcaag gaaagcacgg ccgccctgac cgcgtatatg gccgacttga
     2641 tccaggggtt cctcaagcaa ccgggcgaca acgtcctctc caagctggtg aacgacaagg
     2701 acggtcccga cgggccgatg acgcccaaag aggccgccgc caacgcgatg ctgctactga
     2761 tcgccggaca tgactcgact gtcaacacaa tcgcgcactg cgtgttgaca ctgctccgca
     2821 atcctgagtc gatcgacctg ctgcacgaga acccggaact gatcccgaag gcgatcgagg
     2881 aagtgctgcg gctgcagtcg gccgtgcagt tcttccccag ccgcagcgtc accgccgacg
     2941 tagaggtcgg cggcgtcgtg attccggccg gctccgcagt gcatctgctg tacggcgccg
     3001 ctaatcgcga cccgaaccgg ttccccgacc ctgagaagtt cgacatccag cggccggaca
     3061 atcaacacgt gggctggggc cgcggcgtgc acagctgtgt cggcggacca ctggcgcgcc
     3121 tggaggtcaa catcgccctc gagacgttcc tgcgtcgcgt cgagaatccg cgtctggtcg
     3181 tcgacccacc gccctatcgg gtgaaccagg tgttccgcgg tccgctgcat ctcgaggtcg
     3241 aattcgaccg gatcgcggac tgaggtgaag gcgttccaat tcgtcgaatg gcaacagccg
     3301 gggcaactcc gggacgtacc ggtgccggaa ccgggaccgg gggaggtgct ggtcaaggtc
     3361 ggtggtgccg gcgcgtgcca ttcggacctt catctgttgg aggcccccgc gggatcgaag
     3421 tcgatgacgc tgccgttcac cctcgggcat gagaacgccg gctgggtcga gacgatgggg
     3481 ccgggtgcaa caggtttcgc actcggcgac cccgttatcg tctacggccc gtggggctgc
     3541 gggctgtgca tgaactgccg ccaggggatg gagaattact gccagacgcc cgggaagccg
     3601 agtccgggcg ggctgggtgg catcgacggc ggcatggccg agtacctcgt cgttcctgcg
     3661 acgcgctacc tgattccctt gggcgacttg gacccccgcg aggcagcgcc gctcaccgac
     3721 gcggggttga ccagctatca cgcggtgaag cgatcggcgc atcttttggg gcctggctcc
     3781 acggccgtgg tcatcggcgc aggcggcctt ggccagatgg ccatccaggt gctcaaggcg
     3841 ctggcctcgg cgacaaccgt tgtcgcggtg gatacttcgg ctgacaagct cgagattgcc
     3901 aaaacggtgg gcgccgacga ggtactcgtc tccggcgatg atgccatcaa gaccatccag
     3961 gacatcacca aggggcaggg cgccgaactc gttctcgatc tggtcggagt cgacccgaca
     4021 ctctcgatgg cagctgcggt gtcacgggtg ctcgggcacc tcaccatcgt ggggctcggt
     4081 aacgccgccc tcccggtgaa cttcaacagc ccggcgaagg aatgctccgt cgcgtcgccg
     4141 ttctggggca ccatccccga gctgatcgag gtgatcagcc tcgcccagac aggcaaaatc
     4201 aaaatgctcg tcgaacactt cgccctcgac gatgcagcgc acgcctacca cttgttgcac
     4261 gaaggcaaga tccagggacg cgccgtaatc acaccgaacg actaacggcc cgtgacccgg
     4321 cttctccgtg acaccccgga ttctcgcggg cggaatatgg tccagctaga tgccggtgca
     4381 caacgatacg aacgggatcg gcgcgcagac accgaccgag aagtaaggcg tgaggtcgcc
     4441 gttccacgcg ggcggcggcg gtggcggcgg cggggtggag ggggcaatgg tgctgcgatg
     4501 caggtgttgg acggcggatc gaagatcgtc ccctgaccgc attcgaccgc gtgagcgact
     4561 gcaggcgaac cactgtgatg aatgcaagtg gcgccgacgc ggcggcgcaa atgattgccc
     4621 aacgatgaat gaaggcggcc atggcacgcc cctttgctct tccccaaccc tcttgcgcta
     4681 gcgatgttac gaccggcggc gagctgtttc acaaaaattg acgatcacgg agaatgttcg
     4741 cccgccggct ttcgtttcgg ggccacctca gccgaccttg taggtggcga tcgcctttgc
     4801 aaccaactca ccgtcgggcg tcacgacatc gacgtcgcag ttgaccaggg tcttgccgcg
     4861 ccgcagcacc ctgccgacgc cgatgaggtc cctggcgcgc gcaggcgcga gaaactgcat
     4921 cgagatcgaa gtggtcccgc cgcgcagcga gtcgggcatt tggcactgcc catgcggcgg
     4981 ccatcaccgt catatcggcg agggtggcga tggggtcgcc gtgcaccatg tcgccgagtg
     5041 tgacgttggt gggatcgaac ggcatccgca gccgcacctc gtcgccctcg agcacctcgg
     5101 cgacgatgcc cagcttggcc gcaaggggac aggccgggat gaactgctcc atgacgtctg
     5161 agccggtccg cgctctgttg ttgacatgac ttgaagtgtc ggcggcgcga agcgtttcaa
     5221 ttacctctga ggtagccatg ccccggcctg atcgtgggcc acctctctcg gcgagcagac
     5281 gcaaatgtcc gcgacacgcc gacgcgacac gcgcttttgc gtctgctcgc gcggagaaat
     5341 cgttgacacg ccaggctagg actgctttta taaccggtta taagagtaaa tctggtcaaa
     5401 tctaccaact taacggggaa tagatgacga cggcgcagga gacgaaaccg gccgccgggc
     5461 gctacgagct gagccatctc agggcgctcg aagccgaggc gatccacatc atcagggagg
     5521 tagccgccga attcgagcgg cccgtgctgc tgttctccgg cggcaaggac tccatcgtca
     5581 tgctgcacct
//
LOCUS       DASYLX010000035         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_440974,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000035 DASYLX010000000
VERSION     DASYLX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440974"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="hemQ"
                     /locus_tag="VGP27_00905"
     CDS             complement(<1..561)
                     /gene="hemQ"
                     /locus_tag="VGP27_00905"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEV7849907.1"
                     /translation="MARLDYDELNSTLRYLMFSVFAAEPGALGDDRTAIVDETATFLK
                     QQEDNGVVVRGLYDVAGIRADADFMIWTHADRVESLQTTYSDFRRTTALGRASRPVWS
                     SVALHRPAEFNKSHIPAFLAGEEPGAYICVYPFVRSLEWYLLPDDERRRMLAEHGMAA
                     RGYKDVRANTVPAFALGDYEWILAFEA"
     gene            complement(567..1931)
                     /locus_tag="VGP27_00910"
     CDS             complement(567..1931)
                     /locus_tag="VGP27_00910"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEV7849908.1"
                     /translation="MSASYCVVGGGISGLVAAYRLRVTLGDDADITVFEPADRLGGVL
                     RTEQLGGQPIDIGAEAFVARRPEVPALLAELGLAGRQIGTTGARPLIYSQGRLHELPR
                     GTLNGIPSDPSSMAGLVDDTTIRQMFDEPARPLNWRPGADPAVAELVGDRFGAQVVTR
                     SVDPLLAGVYAGSAASIGLRSAVPTLAAVLDRGARNLTDAVREALPPVTEDSVFGALD
                     GGYAVLLDELIRRADFRWAQVGVDRLESRGSGWQLIDDEGGHWHADAVLLAVPAPRLA
                     TIVAAVAPRTAAAAKQIRVASTALVALALPGGTPLPDRSGVLVTAAERLHAKAITLSS
                     RKWGSRGNVELVRLSFGRFGDSTQDWAHRVGDDDLLSWAADDLAVVFGVTNEPFDCRV
                     HRWIDAMPQYGPGHGRLVAELRAGLPSSLAVAGAYLDGIGVPACIAAANRAAASLVTT
                     RVEN"
     gene            complement(1928..2989)
                     /gene="hemE"
                     /locus_tag="VGP27_00915"
     CDS             complement(1928..2989)
                     /gene="hemE"
                     /locus_tag="VGP27_00915"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEV7849909.1"
                     /translation="MNSRRDLPESPYLAAAGGRKPSRVPVWFMRQAGRSLPEYRALRA
                     ENTMMQACFDAELITEITLQPVRRHGVDAAILFSDIVVPLRASGIELDIVPDVGPVIE
                     HPIRTAADVAGVKPLEAEHVAPVSEAVRQLVAELGDVPLIGFAGAPFTLASYLVEGGP
                     SRHHERTKAMMLGESDTWHALMTVLTDVTTAFLRVQVDAGVDAIQMFDSWAGTLSLAD
                     YRTHVLPHSTRVFEALADVGVPMTHFGVGTAELLGAMSEAVAPAANPVVGVDWRTSLT
                     DAAARVAAGTALQGNLDPVVLMAGIEVAERAARTVVEDGRRAVDAGAAGHVFNLGHGV
                     LPATDPGIITDVVALVHSL"
     gene            3100..3681
                     /locus_tag="VGP27_00920"
     CDS             3100..3681
                     /locus_tag="VGP27_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HEV7849910.1"
                     /translation="MTTAEPAQFREAVAAMNATTVRPEIELGPIRPPQRLAPFSYALG
                     AEVRHPDSAIVPEQSEGDAFGRLILLHDPEGAEAWDGTMRLVAYVQADLDSSEAVDPL
                     LPEVAWSWLVDALEANTEHVTALGGTVTATTSVRYGDISGPPRAHQLELRASWTATTL
                     ELGPHVAAFCVVLEHAAGLPPAGVTDLKSRTRA"
     gene            3683..4945
                     /locus_tag="VGP27_00925"
     CDS             3683..4945
                     /locus_tag="VGP27_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEV7849911.1"
                     /translation="MTEADSGGAEPEDTAEPEATPLLAPADGVPPLSVTAYEIQEAAT
                     LLASGHGPFAVDAERASGFRYSNRAYLVQIRRQGAGTVLIDPVNHGADTPTTLAPVAE
                     VLSSDEWVLHAADQDLPCLAEIGMRPTKLYDTELAGRLAGYDRVNLAAMVARLLGLGL
                     KKGHGAADWSKRPLPPEWLNYAALDVEVLLELQAAIAAVLEEQGKTQWAAQEFEFLRT
                     FEGAPTRRDRWRRTSGIHKVRDRRAMAAVRELWTTRDHIARRRDIAPGRILPDSAIIS
                     AATADPKTVEELTALPVFGGSKQRRSAAVWLDALSRARDDNSQPEVSEPQNGPPPASR
                     WARRKPEAAARLEAARAGLAALSERVSVPTENLVTPEVVRRLCWDWQPSADTEAAVEE
                     FLRDAQARPWQRELVVPVLAAALTTPAQ"
     gene            complement(4929..>5263)
                     /locus_tag="VGP27_00930"
     CDS             complement(4929..>5263)
                     /locus_tag="VGP27_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7849912.1"
                     /translation="PKMFDQFHEILGFNDGKRVTHKVGRFITDRYTHRNRWVRAMRET
                     AVPMRLIDGPADPNSGRHMAQRYLEVIPDADVVMLDDAIGHWPQIEAPEAVLRHFLDH
                     VDRVIAQG"
BASE COUNT          865 a         1862 c         1796 g          740 t
ORIGIN      
        1 tgcttcgaac gcgaggatcc actcgtagtc gccgagcgcg aacgcgggca cggtgttggc
       61 cctgacgtcc ttgtagccgc gggcggccat gccatgttcg gccagcatgc ggcggcgctc
      121 gtcgtcgggc agcaggtacc actccaacga ccggacgaac ggatagacgc agatgtaggc
      181 gcccggctcc tcaccggcca ggaacgcggg gatatgactc ttgttgaact ctgctgggcg
      241 gtgcagcgca accgaactcc acaccggccg agaagcgcgg cccagcgccg tggtgcgacg
      301 gaaatcggag taggtggtct gcagcgactc cacccggtcc gcatgcgtcc agatcatgaa
      361 gtcggcatcg gcacggatgc ccgcgacgtc atagaggccg cggaccacga ccccgttgtc
      421 ctcctgctgt ttgaggaacg tcgcggtctc atccacgatc gccgtccgat cgtcgccgag
      481 ggcacccggc tccgcggcga agacggagaa catcaggtag cgcagcgtcg agttgagttc
      541 gtcgtaatcc aggcgggcca taacccctag ttttccacgc gcgtcgtgac cagagacgcc
      601 gccgccctgt tggccgcggc gatgcaggcg ggcaccccga ttccgtccag gtacgcgccc
      661 gccaccgcca gcgatgacgg aaggcccgcg cgcagttcgg ccaccaggcg cccgtgcccc
      721 ggcccgtact gcggcatcgc gtcgatccac cggtgcaccc ggcagtcgaa cggctcattg
      781 gtgacaccga aaacgaccgc gagatcgtcg gccgcccacg acagcaggtc gtcgtcaccg
      841 acacggtgag cccagtcctg ggtggaatcg ccgaaccgac cgaacgacag ccgtaccagc
      901 tcgacgttgc ctcgcgatcc ccacttacgc gacgacagcg tgatggcctt ggcatgcagg
      961 cgttctgctg cggtcacgag cacacccgaa cggtccggca gcggggtgcc gccgggcagt
     1021 gccagcgcga ccagcgcggt cgacgccacc ctgatctgtt tggccgcggc ggccgtgcga
     1081 ggggcgacgg cagcgacgat cgtcgccaac cggggcgcag ggaccgccag cagtaccgcg
     1141 tcggcgtgcc agtggccgcc ctcgtcgtcg atcaattgcc agccgctccc ccggctctcg
     1201 agccggtcga cgcccacctg cgcccaccgg aagtcggcac gccggatcaa ctcgtcgagc
     1261 agcacggcat aaccgccgtc gagcgcaccg aacacggaat cctccgtgac cggcggcagc
     1321 gcctccctga cggcgtcggt cagattccgg gcgccgcggt cgagcaccgc ggccagagtc
     1381 ggcaccgcag acctcagccc gatggaggcg gccgaccccg cgtagacgcc tgccaacaac
     1441 gggtcgaccg aacgggtcac cacctgtgcg ccgaaccggt cgccgacgag ttcggctacc
     1501 gcgggatcgg ccccgggccg ccagttcagc ggcctcgccg gctcatcgaa catctgccgg
     1561 atcgtggtgt cgtcgaccag tccggccatc gacgacgggt cggacggaat gccgttgagc
     1621 gtgccgcggg gcagttcatg caggcgcccc tggctgtaga tcaatggccg cgcgcccgtg
     1681 gtgccgatct gcctgcctgc gaggcccagc tcggcgagca gcgcaggcac ctcggggcgg
     1741 cgggcgacga aagcctcggc gccgatgtcg atgggctggc cgccgagctg ctcggtgcgc
     1801 aacactccgc cgagccggtc ggcgggctcg aacaccgtga tgtccgcgtc gtctccaagg
     1861 gtgacgcgga gccggtaagc ggcgacgagt cccgaaatac cgcctccgac aacgcaatag
     1921 gatgcgctca cagcgagtga accaacgcaa ccacgtcggt gatgatgcct gggtcggtcg
     1981 cgggcagcac accatgaccg aggttgaaca cgtggcccgc cgcgcccgcg tcgaccgcac
     2041 gacggccgtc ctcgaccacc gtgcgtgccg cgcgctcggc cacctcaatg cctgccatca
     2101 gcaccaccgg atcgaggttg ccctgcaggg cggtgcctgc cgccacccgg gccgcggcgt
     2161 cggtcagcga ggtacgccag tccacgccga ccaccgggtt ggcggcgggc gccacggcct
     2221 ccgacatcgc gccgagcagt tcggccgtgc ccacaccgaa gtgcgtcatc ggcaccccga
     2281 catcggcaag cgcctcgaac acccgcgtgc tgtgcggcag cacgtgggtg cggtagtcgg
     2341 ccagcgacag cgtcccggcc caggagtcga acatctggat ggcgtcgacg cctgcgtcca
     2401 cctgcacccg cagaaacgcg gtggtgacgt ccgtcagcac cgtcatcagc gcatgccagg
     2461 tgtcggattc gccgagcatc atcgccttgg tgcgctcgtg gtggcggctc ggaccgccct
     2521 cgacaaggta ggacgccagc gtgaaggggg cacccgcgaa gccgatcaat ggaacgtcgc
     2581 ccagctcggc gaccaactgc cgcacggcct cggaaacggg cgcgacgtgc tcggcctcca
     2641 ggggtttcac accggcgacg tccgcggcgg tgcggatcgg gtgctcgatg accggcccga
     2701 cgtcgggcac gatgtccagc tcgatgcccg acgcccgaag cggtacgacg atgtcggaga
     2761 acaggatggc ggcgtccacg ccgtgcctgc gcaccggctg taacgtgatt tcggtgatca
     2821 gctctgcgtc gaagcaggcc tgcatcatcg tgttctccgc gcgcagggca cggtactcgg
     2881 gcagcgaacg gcccgcctgc cgcatgaacc acacgggaac ccggcttggc ttacggccac
     2941 cggcggcggc caggtacggc gactccggca ggtcacgacg gctattcatc gacatcaatg
     3001 ctgccatgac agccagactg cgggcagttc ggcgcccctg ctcgaccaga tacggcgcgc
     3061 ctgcgcacag gcctgctcaa atggtctacc gtcaagtcgg tgaccaccgc cgaaccggct
     3121 cagttccggg aagcggtcgc ggcgatgaat gccactaccg tgcgaccgga aatcgagctg
     3181 ggcccgatcc ggccgccgca gcgtctggcg ccgttcagct acgccctcgg cgccgaggtg
     3241 cgtcatcccg actccgccat cgtgcccgag cagtccgagg gggacgcctt cggacgactg
     3301 atcctgctgc acgacccaga gggcgcagaa gcctgggacg gcaccatgcg gttggtcgcc
     3361 tacgtccagg cggacctgga ttccagcgag gcggtcgacc cgctgctgcc cgaggtcgcc
     3421 tggagctggc tggtcgacgc gctggaggcg aacaccgagc acgtcaccgc tctcggcggc
     3481 accgtcaccg ccaccacatc ggtgcgctac ggcgacatct ccggcccgcc tcgggctcat
     3541 cagttggagc tgcgcgcatc ctggaccgcg acgacgctgg agctgggccc gcatgtggcc
     3601 gccttctgcg tagtgctcga gcacgccgca ggtctgccgc ctgccggtgt gaccgatctg
     3661 aagtcgcgca cccgcgcttg agatgaccga agccgactcc ggcggagccg agccagagga
     3721 caccgcagaa cccgaagcga cgccgctgct ggcgcctgcc gacggagtgc caccactatc
     3781 ggtcacggcg tatgaaatac aagaagcggc aacgcttctg gcgtccggac acgggccgtt
     3841 cgcggtcgac gccgaacggg cgtcggggtt tcgctactcg aaccgggcgt acctggtgca
     3901 gatccgccgc cagggtgcgg gcacggtgct gatcgatccg gtcaaccacg gtgccgacac
     3961 gcctacgacg ctggcgccgg tcgcggaggt gctttccagc gacgagtggg tgctgcacgc
     4021 cgccgaccag gacctgccgt gcctcgccga aatcggcatg cggccgacga agctctacga
     4081 caccgaactg gcgggccggc tggccgggta cgaccgggtg aacctggcgg cgatggtggc
     4141 gcggctgctg ggcctcggcc tgaagaaggg gcacggcgcc gccgactggt ccaagcggcc
     4201 gctgccgccg gagtggctga actacgccgc cctcgacgtg gaggtactgc tcgaactgca
     4261 ggccgccatc gcggccgtgc tggaggagca gggcaagacc caatgggccg cccaggagtt
     4321 cgagttcctg cgcaccttcg aaggggcacc gacgcgacgg gaccgttggc gccgcacctc
     4381 aggcatccac aaggtgcgcg accgccgcgc gatggccgcc gtgcgcgagc tgtggaccac
     4441 ccgagaccac atcgcccgca ggcgggatat cgcaccgggc cgcatcctgc ccgattcggc
     4501 gatcatcagc gcagccaccg ccgaccccaa gaccgtcgag gagctcaccg cactgcccgt
     4561 cttcggtggg tccaaacagc ggcgcagcgc tgcggtctgg ttggacgcgt tgagccgggc
     4621 ccgtgacgac aacagccagc cggaggtctc cgagccgcag aacggccccc cgcctgcgtc
     4681 acggtgggcc cgtcgcaaac ccgaggccgc ggcgcggctc gaggcggccc gcgccggcct
     4741 cgccgcactg tccgagcggg tgtcggtgcc cacagagaac ctggtgaccc cggaggtggt
     4801 acgccgattg tgctgggact ggcagccgtc ggccgacacc gaggcagccg tcgaggagtt
     4861 cctgcgcgat gcccaggcca ggccgtggca acgcgaactg gtggtgccag tgctcgccgc
     4921 ggcgctgact acccctgcgc aatgacccgg tcgacgtgat cgaggaagtg cctcagcacc
     4981 gcttctggcg cctcgatctg gggccagtgc ccgatcgcgt catcgagcat gacgacatcc
     5041 gcgtcgggaa tcacttcgag atagcgctgg gccatatgcc tgccggagtt cgggtcggcg
     5101 ggtccgtcga tcaaccgcat cggcacggcg gtctcgcgca tggctcgcac ccaccggttg
     5161 cggtgtgtgt agcggtcggt gatgaaccgc ccgactttgt gggtcacgcg cttgccgtcg
     5221 ttgaaaccga ggatttcgtg gaactggtcg aacatcttcg gcg
//
LOCUS       DASYLX010000036         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1538174,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000036 DASYLX010000000
VERSION     DASYLX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538174"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGP27_00935"
     CDS             <1..429
                     /locus_tag="VGP27_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7849913.1"
                     /translation="VINISSVFGLLAVPGQGAYNAAKFAVRGFTEALRQEMLVAGRPV
                     KVTCVHPGGIKTAIARNSAAVEGVDQAELAKFFDKKLASTSAQKAARVILDGVRKNKA
                     RVLVGNDAKALDLLARVTGSGYQRVVAAVTSRQGPTSSLA"
     gene            complement(571..1416)
                     /locus_tag="VGP27_00940"
     CDS             complement(571..1416)
                     /locus_tag="VGP27_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7849914.1"
                     /translation="MAARRLTVALIASFLVAGCGGTTPSQSVAVGARDDVESTLLANL
                     YAAALRSYGTDAHVEVVPDPLTGLDSGDVRVAPGFTGRLLNTFAPDARARAAVQVYRD
                     MVSALPEGVAAGDYAESAEDKPALAVTEGTVDAWGGRDVTAAVRNCDKLKIGRVVGVR
                     NPGAIGTCVPAVRDYRDAARLFDALRIGQVTAAWTSTADPDIPADALVLADRTALIRA
                     ENVVPLYRRNELTEMQELALNEVAGVLDTAGLVQMRRQVAAGTDPAHVADGYLAEHPL
                     GAASR"
     gene            complement(1424..>1849)
                     /locus_tag="VGP27_00945"
     CDS             complement(1424..>1849)
                     /locus_tag="VGP27_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEV7849915.1"
                     /translation="DGLSGGMVEALKGADVFLGLSGGVVPEELIATMAADGIVFALSN
                     PDPEIHPEVAAKYAAVVATGRSDFLNQINNVLAFPGVFRGALDAGARRITEKMKVAAA
                     EAIFGVVGDDLAREYIVPSPLDPRVGPAVAAAVAAAADA"
BASE COUNT          291 a          654 c          630 g          274 t
ORIGIN      
        1 gtcatcaaca tctccagtgt cttcgggttg ctcgctgtgc cggggcaggg cgcctacaac
       61 gcggccaagt tcgcggtgcg cgggttcacc gaagcgctgc gtcaggagat gctcgtcgcg
      121 ggccgtccgg tcaaggtgac gtgcgtgcac cccggtggca tcaagaccgc gatcgcccgc
      181 aactccgccg ccgttgaggg tgtggaccag gcggaactcg cgaagttctt cgataagaag
      241 cttgcgagca cgtctgcgca gaaagccgca cgggtgatcc tggacggcgt ccgtaagaac
      301 aaggcccggg tgctggtcgg caatgatgcg aaggcactcg acctcttggc gcgggtgacc
      361 ggttccggct atcagcgggt cgttgccgct gtgacatccc gacaagggcc caccagctcc
      421 ctggcttgac gcacgtgcct taagggcgcg ctcgtcggcc gcgaaggcgc gcagaatgcc
      481 gcgattctgc ggcgtgtcgt gtgtaaacac gctccccctt gcacgcgggg aggtaccccc
      541 agctcgcggt gcggggccgg cgggagaggg ttagcggctg gccgcaccca acgggtgttc
      601 ggcaaggtaa ccgtcggcga cgtgggcggg atcggtgccc gctgcgacct gccgccgcat
      661 ctgtaccaac ccggcggtgt ccagcacgcc cgccacctcg ttgagcgcga gctcctgcat
      721 ctcggtgagc tcgttgcggc ggtacagggg cacgacgttc tccgcgcgaa tcagcgccgt
      781 gcggtccgcc agcaccagcg cgtctgcggg aatgtccggg tcggccgtcg acgtccacgc
      841 cgcggtcacc tgcccgatcc gcagcgcatc gaaaagcctt gcggcatccc ggtaatcgcg
      901 caccgcgggc acgcaggtcc cgatggcccc ggggttgcga acgccgacca cccggccgat
      961 cttcagcttg tcgcagttgc ggaccgccgc ggtcacatca cgtccgcccc acgcgtcgac
     1021 ggtgccctcg gtgaccgcga gcgccggctt gtcctccgcc gactccgcgt aatcgcctgc
     1081 ggccacgccc tcgggcaggg cagacaccat gtcgcggtaa acctgaacag cggcacgcgc
     1141 cctggcgtcg ggcgcaaagg tgttcagcag ccgaccggtg aatcccggcg ccacccggac
     1201 gtcgcccgag tccaggcctg tcaacgggtc gggcactacc tcgacatgcg cgtcggtgcc
     1261 gtaggaccgc agcgcagcgg cgtagaggtt ggccagcagc gtcgactcca cgtcgtcacg
     1321 cgccccgacg gcgaccgatt ggctgggcgt cgttccgccg cagcccgcta cgaggaacga
     1381 agcaatcagc gcgacggtga gccgacgggc ggccatcggg tcgtcaggcg tccgcggctg
     1441 ctgcgaccgc cgcggccacg gcgggcccga cccgcgggtc cagcgggctc ggaacgatgt
     1501 actcgcgagc caggtcgtcg cccaccaccc cgaagatcgc ttcggcggca gcgaccttca
     1561 tcttctcggt gatccggcgg gcgcccgcgt ccagcgcacc gcggaacacg cccgggaacg
     1621 ccagcacgtt gttgatctgg ttcaggaagt cgctacggcc agtcgccacc acggcggcat
     1681 acttggccgc gacctccgga tggatctccg ggtcggggtt ggacaatgcg aacacgatgc
     1741 cgtcggcagc catcgtcgcg atcaactcct cgggcacgac gccacccgaa agcccgagga
     1801 acacgtcggc gcccttgagc gcctcgacca tgccgccgga aaggccatc
//
LOCUS       DASYLX010000037         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2854634,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000037 DASYLX010000000
VERSION     DASYLX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854634"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VGP27_00950"
     CDS             <1..523
                     /locus_tag="VGP27_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEV7849916.1"
                     /translation="EGSALVYLSLGSLGGADVELMQRLVDVLGTTEHRYIVSKGPQAD
                     RITLADNMVGAQMLPQTKVIPQVDLVISHGGNNTVTETLHFGKPLIVLPLFWDQYENA
                     QRIDELGFGVRLDTYAFADDELTGAVDRLLADTELRNKLDEMGAAIRSRDGLRVGADV
                     IEEVGVKHRASVG"
     gene            516..1721
                     /locus_tag="VGP27_00955"
     CDS             516..1721
                     /locus_tag="VGP27_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047931 - glucosamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine kinase"
                     /protein_id="HEV7849917.1"
                     /translation="MAETLDALDLTDGKRLAIVADGDGLAALPMVREPDGRWRRARAG
                     DGVAEALLNVLARQAGPATEGRFTIQSWIQRAALGERAIDVDQTNESVIVGDSAVVKW
                     APHLQEGPHPAPHRVAVLRRAGFAGMPAPWGLVTWQPADGEKTLAASVDEYLPGAVDG
                     WTWAVALITDAARDRRPGPAVDAAADVGALVAELHAALAGTATTASQGDADRWRDAAC
                     ETLQIVCGLADHAAVSITRARRRDVEVVLHRLGTLAGIPVIEGHGDLHVGQVLLADGR
                     FVVTDFDGNPVLRATERMLPIPAALDVAGMTQSFAHAAVVAGRYTALDAATLAQTDAL
                     VRSAFLRSYADRIAALGHGELYDPVALYPFRMQQVLREIIYAARYLPRWMYVPDAALP
                     ALLGEGTAT"
     gene            1718..2686
                     /locus_tag="VGP27_00960"
     CDS             1718..2686
                     /locus_tag="VGP27_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV7849918.1"
                     /translation="MKPDGFAADLYRKPEVLQRLAAKLSDGNPWREVVPPDTERVVLL
                     GMGSSAYAGGVAAARMRARGIVASSELASSQLLPDWQDGTLVVATSAGGGSAETLDAL
                     GRLPGSVHSVALTNTAGSAITQRCDAVVNLAAEPEAGGVACRSYQHTLAMLLALECHL
                     AGDGTEALAHAVGLTADASAYLLDTETDWRLEVSEPLLGSTGTYLAAPAHRFCSAQQG
                     ALMLREGPLLPAVWCETGDWSRVDVYLTKTTDYRLLVFAGSRWEEQLAEWTTQRDSTV
                     VGIGGVVPGAHYNLRYPGDSNDVVRLLAEVLVPELMAARAWQAQRM"
     gene            complement(2674..>3034)
                     /locus_tag="VGP27_00965"
     CDS             complement(2674..>3034)
                     /locus_tag="VGP27_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849919.1"
                     /translation="PERGLQVKTILASRAISALFPEIHNIGGVRPDALRWHPNGLALD
                     VMIPNPTSTDGIALGNRIVAFALENADKFDLQDCIWRGVYYTPSGAKAGGYGHFDHVH
                     ITTHGGGYPTGDEVYIR"
BASE COUNT          485 a          988 c         1068 g          493 t
ORIGIN      
        1 cgaaggcagc gcactggtct acctctcgct cggttcgctc ggcggcgcag acgtcgaact
       61 gatgcagcgc ctggtcgacg tgctcggcac caccgaacac cggtacatag tgagcaaggg
      121 cccgcaggct gatcgaatca ccctggcgga caacatggtc ggtgcgcaga tgctcccgca
      181 gacgaaggtg attccgcagg tcgacctggt gatctcgcac ggcgggaaca acaccgtcac
      241 cgagacgctg cacttcggca agccgctgat cgtgctgcca ttgttctggg atcagtatga
      301 gaacgcccag cgcatagacg aactcgggtt cggcgtgcgg ctggacacct acgcgttcgc
      361 cgacgatgag ctcacgggcg cagtggaccg actgctggcc gataccgagc tacgcaacaa
      421 gctcgatgag atgggcgccg cgatccgttc acgcgacggc cttcgggtgg gggccgacgt
      481 catcgaagag gtgggtgtga agcatcgggc ttcggttggc tgagacgctc gacgcgctcg
      541 acctcaccga tggcaagcga ttggccatcg tcgccgacgg cgacgggctg gcggcactcc
      601 cgatggtgcg cgagccggac ggccggtggc gtcgcgcccg ggcgggtgac ggagtggccg
      661 aggcactgtt gaacgtactg gcgcgacaag ccgggccggc gaccgagggc cgcttcacga
      721 ttcaatcctg gattcagcgg gcagcgttgg gcgagagggc aatcgatgtc gaccagacca
      781 acgagtcggt gatagtcggc gacagcgcgg tggtcaaatg ggcgccccat cttcaggagg
      841 ggccgcaccc ggcgccgcac cgggtagcgg tgctgcgcag agccggtttt gccggcatgc
      901 ccgcgccgtg gggtctcgtc acgtggcaac cggcggacgg tgagaaaacg cttgcggcaa
      961 gtgtcgacga gtaccttccc ggcgcggtgg acggctggac gtgggcggtg gcgctgatca
     1021 ccgacgctgc ccgtgaccgt cgccccggcc cggcggtcga cgcggcggcc gacgtcggcg
     1081 ccttggtcgc cgagctacac gccgcgctgg ccggcacggc gaccaccgcc tcgcaggggg
     1141 atgcggaccg atggcgcgac gccgcgtgcg agacgctgca aatcgtttgc ggcttagccg
     1201 atcacgcagc cgtgtcgatc acgcgcgcgc gccggcgcga cgtggaggtg gttctgcatc
     1261 gtctcggcac actcgcaggc atccccgtca tcgagggtca cggtgatttg cacgtaggtc
     1321 aggtgctgct ggccgacggc cgtttcgtgg tgaccgattt cgacggcaac cccgtactgc
     1381 gggcgacgga gcggatgctg ccgatacccg cggccctcga cgtcgccgga atgacccagt
     1441 cgttcgcgca cgccgccgtc gtcgccggca gatacacagc gctggacgcc gcgacactcg
     1501 cccagaccga cgcactggtt cggtcggctt tcctgcggtc ctatgccgac cggatcgctg
     1561 cgctcggtca cggtgagctc tacgaccctg tcgcgctgta cccgttccgc atgcagcagg
     1621 tactgcgcga gatcatctac gccgcacgat atctgccgag atggatgtac gtgcccgacg
     1681 cggcacttcc cgctctgctc ggtgaaggga ccgcaacgtg aagccggatg gtttcgccgc
     1741 cgatctgtat cgcaagcccg aggtcctgca acggctcgcc gcgaagctgt cggacggaaa
     1801 cccgtggagg gaggtcgtcc cacccgacac cgagcgggta gtcctgctgg gcatggggtc
     1861 gtcggcatac gccggcggtg tggcggcggc gcgcatgcgt gcccgcggga tcgttgcgtc
     1921 gtccgaactc gcttcctccc aactgcttcc ggactggcag gacggaacgc tggtcgtcgc
     1981 gacatcggcc ggcgggggct cggcggagac cctggatgcg ctgggtcgcc tgcccgggtc
     2041 ggtgcactcg gtagcgctga cgaacaccgc gggatcggcg atcacccagc gttgcgacgc
     2101 agtggtgaac ctggcagccg aacccgaagc cggcggcgtg gcatgtcgca gctaccagca
     2161 caccctggcc atgctgctgg cgctcgaatg tcatctcgcc ggcgacggca cggaggccct
     2221 cgcgcacgcc gtcgggctga cggcggacgc aagcgcttac ctgctcgaca ccgaaaccga
     2281 ttggcgtctg gaggtttccg aacccctcct cggttcgacc ggcacctacc tggcggcacc
     2341 ggcgcaccgg ttctgttcgg cccaacaggg cgcgctgatg ctccgcgaag gtcccctgct
     2401 accggcggtc tggtgcgaaa ccggcgactg gagccgtgtc gacgtgtact tgacgaagac
     2461 caccgactac cggctgctcg tattcgcggg gtcccgatgg gaagaacagc tggccgagtg
     2521 gacgacgcaa cgcgacagta ccgtcgtcgg catcggcggg gtggtgcccg gagcgcacta
     2581 caacctgcgt tatccgggcg actcgaatga tgtcgttcgg ctacttgccg aggtgctcgt
     2641 gcccgagctc atggccgctc gggcgtggca ggctcagcgg atgtagacct cgtcgccggt
     2701 gggatacccg ccaccgtgcg tcgtgatgtg gacgtggtcg aagtggccgt acccgccggc
     2761 cttggcaccg ctgggcgtgt agtagacgcc ccgccagatg cagtcctgaa gatcgaactt
     2821 atccgcgttc tcgagcgcga acgcgacgat ccggttgcca agcgcaatgc cgtccgtcga
     2881 cgtcgggttg ggaatcatca cgtcgagcgc caggccgttg gggtgccatc tgagcgcatc
     2941 ggggcggaca ccgccgatgt tgtggatctc cggaaacagc gcgctgatgg cgcgcgacgc
     3001 caggatggtc ttgacctgga gaccccgttc cggg
//
LOCUS       DASYLX010000038         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2196287,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000038 DASYLX010000000
VERSION     DASYLX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196287"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VGP27_00970"
     CDS             <1..176
                     /locus_tag="VGP27_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEV7849920.1"
                     /translation="DLIPDFIPVIGYADDAIIIALVLRSVTRAAGPAALDEHWPGTPE
                     GLAAVRRLCRLPE"
     gene            complement(384..629)
                     /locus_tag="VGP27_00975"
     CDS             complement(384..629)
                     /locus_tag="VGP27_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849921.1"
                     /translation="MTAAAALAFIIAPTAVFMSSDDGTSAQADSCYNGVTVLNPYGKS
                     CTLPGPANKIRGSAPDANAIIACRHHPGCLAWYVNNP"
     gene            844..1197
                     /locus_tag="VGP27_00980"
     CDS             844..1197
                     /locus_tag="VGP27_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7849922.1"
                     /translation="MRAKRITANLRVSDIDAVKSFYTDFLGLSTEEFNMGWVARYTSP
                     DTGANVQLVTKDATAAEDSVISVHTDDIEAAYEEAKEHGFEIVHPLSTEAWGVRRFLV
                     RAPDGNVINVVHHRE"
     gene            1247..1993
                     /locus_tag="VGP27_00985"
     CDS             1247..1993
                     /locus_tag="VGP27_00985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7849923.1"
                     /translation="MSFVVSPDAYARFMGRYAEPLAGVFAAFAGVGVDDKVLDVGCGP
                     GALTAHLLSVGAEVAAIDPSPPFVDAIRTRFPEVEVCRGTAEELPYDTAAFDVALAQL
                     VVHFMSDPVVAVRQMARVTRRGGVIAACVWDGPTGALAPFWDAVHVIDPDAEDEALLS
                     GAETGHLTELFVEAGLRDVQEESIAVELVHPTFQEWWEPYTFGVGPAGDYVQRLDDDG
                     RDRLESVAHERLGSGPFTVTATAWAARGVV"
     gene            complement(2120..2557)
                     /locus_tag="VGP27_00990"
     CDS             complement(2120..2557)
                     /locus_tag="VGP27_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849924.1"
                     /translation="MTGSDDGENPVFHVGQVYPRISDYDRAWSLDGVAVIKLIEESCF
                     WGFLGWFDANIWPSLVEATVKYPQRIRAADLPLQITGWNVRRSGDFDVTVAYEIRPEA
                     TPDSEPVFSAEIRWQGFEMGPFEGEATLLSGPSDSRSSSGPMF"
     gene            complement(2544..2921)
                     /locus_tag="VGP27_00995"
     CDS             complement(2544..2921)
                     /locus_tag="VGP27_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7849925.1"
                     /translation="MPSQRPSRAVRYSRRRRRRIAAKVHDLTDAQWAALKSAWGGCAY
                     CGAVGTSLQKDCVLPISRGGRYVLANVVPCCRSCNTSKCNTEVTSWMRRKKLDEGRFL
                     LRQAEIKRVLATDREKGEMNDRI"
     gene            3132..4211
                     /locus_tag="VGP27_01000"
     CDS             3132..4211
                     /locus_tag="VGP27_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEV7849926.1"
                     /translation="MTTPPPPPQRPGQPGRQGRGDPRQDPATRRFQRPGRPPEPPEPP
                     TEQLRARRQPADAAPTERFRPPPPPPAPPSGGPPLQPPAGGVPPPPPRGTPPPPPPPA
                     AAPPQDTEPPAATKRRGRGFLNKTSIILIAVIIVALVTGGLAGAELYARHRADGVLVE
                     VAECVVRDGVTISYGVNPPFLWQHITGHYTNISVSTAGHNVQDAKGMTADVTVSDVRL
                     RDTGDSKGTIGSLTATLDWKSAGIKDTLAANLPAVGNLITGVRTDPAASTIVVDAGDN
                     SVTAKPVVTEGDLNLEVLDVTGPLPKDAVQSALNDLTKKLNDNYPLGIHADSVAVTDT
                     GVVGKFSSQNASIPKENANACFARL"
BASE COUNT          789 a         1355 c         1359 g          758 t
ORIGIN      
        1 tagatctcat cccggacttc atccccgtaa ttgggtacgc agacgacgcc atcatcatcg
       61 cgttagtttt gcggtctgtg acccgggctg ccgggcctgc cgcacttgat gagcactggc
      121 caggcacacc cgaaggactt gcggccgtgc gccgtctgtg cagactgccc gaatgagtcg
      181 gcccgcaaac ggggagtggt gacgggccga cgttggggag cgttaggtca gatgggcggc
      241 gacatgcccc acgaggacct ggttcggggg cacaacacct gcccgtgggg caacggctcg
      301 gcggcttgac gatctaaagg ccgcgtgtag tgcggaccaa tccgccccga atacacgttc
      361 aacagcctat tgccgtgtgc ctcttagggg ttgttcacgt accaggcgag gcagcccgga
      421 tgatgtcggc acgcgatgat ggcattggcg tcgggggctg aaccgcggat cttgttggcg
      481 ggaccgggaa gagtgcagct ctttccgtac gggttgagaa cggtgacgcc gttgtagcag
      541 ctgtcggcct gagccgaagt accgtcatcg ctggacatga aaactgccgt cggcgcaatg
      601 atgaaggcca gtgcggccgc cgcggtcata acccatcgga gtaagcgtcg ccatctcaca
      661 cttatggcaa ctacttcgat aagctatgca ttcccctagc tcctgcagag aaattcgcac
      721 gagtcggccc gccgtccgtg gggatttagg gtggcgggct gaggggagca ttgatgagac
      781 ggtgtggatg ccgaatcggt tcccgttccg cagtggatcc gcaatgcatc tgaggagaaa
      841 accatgcgcg ccaaacgcat caccgccaac cttcgagtgt cggacatcga cgcggtcaag
      901 agtttctaca cggacttctt agggctgagc accgaagagt tcaacatggg atgggtggcc
      961 cgctacacgt cgcccgacac cggagcgaac gtccagctgg tgaccaaaga tgcaacagcc
     1021 gccgaagact cggtcatctc cgtccacacc gatgacatcg aagctgccta cgaagaggcc
     1081 aaagaacacg gcttcgaaat cgtgcacccg ctgtcgaccg aagcgtgggg tgttcgtcga
     1141 ttcctggtcc gcgcaccaga tggcaacgtc atcaacgtcg tccatcaccg agagtgattc
     1201 cgtcatcgcg caaaaaatga ctcaaccggc gacgtagcat tcgttggtga gcttcgtcgt
     1261 ctcgccggac gcctatgcac gtttcatggg ccgctacgcc gagcctcttg cgggggtctt
     1321 cgcggcgttc gccggagtcg gtgttgacga caaagtcctc gatgtcggtt gtgggccggg
     1381 cgctctgacg gcgcatctgc tgtcggtcgg agccgaggtc gcagcgatcg acccttcgcc
     1441 gccgttcgtt gacgcgatcc gtacgcgctt tcccgaggtt gaggtctgtc gagggacggc
     1501 ggaggaattg ccttacgaca ccgccgcttt cgacgtggcg ctcgcgcaac tggtggtgca
     1561 cttcatgtcg gacccagtgg tcgcagtcag gcagatggcg cgcgtgaccc ggcgtggcgg
     1621 tgtcatcgcg gcatgtgtgt gggacgggcc gaccggagcg ctggcgcctt tctgggatgc
     1681 ggtacacgtg atcgatcctg acgccgaaga tgaagcactt ctctcgggtg cggagacggg
     1741 gcatctgact gaactgttcg tagaggccgg actgcgcgat gtgcaagagg aatccatcgc
     1801 agtcgagctc gttcatccga cctttcagga gtggtgggag ccctacacat tcggggtcgg
     1861 tcctgctggt gactacgtcc agcgactcga tgacgatggt cgcgatcggc tcgaatcggt
     1921 ggctcacgaa cgactgggca gtggcccgtt caccgttacc gcaacggcgt gggccgcccg
     1981 cggggtggtg taatcattcg ccgctggtgg ccgccgacgt tcagaaaacc agtgccggac
     2041 ttgctcgtac gccgcttcgc gttgcgtcaa catttgatga cggcttgcaa aaggacaccg
     2101 ccgatgcgac ttccggacgt cagaacatcg gaccgctcga cgaccgggag tcgctgggac
     2161 ccgacagaag cgtcgcctct ccttcgaaag gacccatctc gaacccctgc cagcggatct
     2221 cggcactgaa gaccggttcg gagtcgggcg tggcctccgg tcgaatctcg taggccaccg
     2281 tgacatcgaa gtcacccgat ctgcggacgt tccagccggt gatctgcaga gggaggtcgg
     2341 ccgcacggat ccgctgcggg tatttcaccg tcgcctccac caacgatggc catatgttcg
     2401 cgtcgaacca accgagaaaa ccccagaagc aggattcctc gattagcttg atcactgcga
     2461 caccgtcgag ggaccaggcc cggtcgtagt cacttattcg cggatatacc tgtccgacgt
     2521 ggaacacagg gttttcaccg tcgtcagatc ctgtcattca tttctccctt ttcgcggtcg
     2581 gtggccaaga cccgtttgat ctcggcctgc cgcaatagga atcgcccttc gtccagtttc
     2641 tttcttcgca tccagctggt cacctcggtg ttgcacttgc tggtgttgca tgagcggcag
     2701 cagggcacaa cattggcgag cacatatcgg cctccgcgcg agatgggcag cacgcaatcc
     2761 ttctggagcg acgtcccgac ggcgccgcag taggcgcaac cgccccaggc tgatttcagt
     2821 gcggcccatt gagcgtcggt gaggtcgtgc actttcgcag cgatacgccg cctgcgacgg
     2881 cgcgaatacc gcaccgcgcg cgacggtctc tgcgacggca tggccgaatt atcgcggtga
     2941 cggctgcatt caggcgcggg gcgttcggcg tgtcgtggtg tgactacctg gattgcgtcc
     3001 tgttgccggt tcctgaccgt cgccaggagc gtcggaggct gggaaacgtc gggccagcag
     3061 tactcctgtt ctcgaccgcc ttgatgggga ttaccgcgag ctatccagcg tgatgaggca
     3121 gaatcgcgca ggtgaccacg ccgccgccgc cgccgcaacg gccggggcag cccggccggc
     3181 agggcagggg agacccgcgc caggatcctg caacgcgacg gtttcagcgc ccgggcaggc
     3241 cgcctgaacc gcccgaaccg ccgaccgagc agctccgtgc gcggagacag cctgccgatg
     3301 cggcgccgac cgagaggttc cgaccgccac cgccaccgcc ggcgccgcct tcgggtgggc
     3361 cgccgcttca gcctcccgcc ggtggcgtgc caccgccacc accgcgcggc accccgccac
     3421 caccaccgcc gcctgccgcg gcgccgcccc aagacaccga gccaccggca gccacgaagc
     3481 ggcggggcag gggttttctc aacaagacgt cgatcatcct gatcgcggtc atcatcgtcg
     3541 cgctggtgac aggcggcctg gcaggcgccg agctctacgc ccgccaccgc gccgacggcg
     3601 tgctcgtgga agttgccgaa tgcgtcgttc gcgacggcgt cacgatctcg tacggagtga
     3661 acccgccatt cctgtggcag cacatcaccg gccactacac caacatctcg gtctccactg
     3721 ccgggcacaa tgtccaggac gcgaaaggca tgacggccga cgtgaccgtc tcggatgtcc
     3781 gattgcggga taccggtgac tccaagggca cgatcgggtc gctgacggcg acactcgact
     3841 ggaagtccgc cggtatcaag gacaccctgg cggccaacct gccggcggtc ggcaacctga
     3901 tcaccggtgt gcgcaccgac cccgcggcct ccacgatcgt cgtcgacgcc ggagacaaca
     3961 gcgtcacggc caaacccgtt gtcaccgaag gtgatctgaa cctcgaggtg ctcgacgtca
     4021 ccggaccgct gccgaaggat gcggtgcagt cagcgctcaa cgacctgacc aagaagctca
     4081 atgacaacta tccgctcgga atccacgccg acagcgtcgc agtcaccgac accggtgtcg
     4141 tcgggaagtt ctcgagccaa aacgcgtcga tcccgaagga gaacgcgaac gcctgcttcg
     4201 cgagactgta ggaaaggcta tcgcgcgatc aggcggaaga cgggaatcgc gcgtccaacg
     4261 g
//
LOCUS       DASYLX010000039         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3622473,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000039 DASYLX010000000
VERSION     DASYLX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3622473"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGP27_01005"
     CDS             complement(<1..424)
                     /locus_tag="VGP27_01005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7849927.1"
                     /translation="MTDADDTTDNPFFARLWTFLSNHETEEIQRLRTENLAGLTGRVL
                     EVGAGTGTNFACYPATVTEVVAVEPERRLAPQARKAAAKASVPVTVSTGTVEKFLSDG
                     DGKAFDAVVCSLVLCSIDDPDSVLRQLFSLVRPGGELRF"
     gene            complement(421..1119)
                     /locus_tag="VGP27_01010"
     CDS             complement(421..1119)
                     /locus_tag="VGP27_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7849928.1"
                     /translation="MSLQPLKRRRAPRGSGEQLRNEILDATTRLLLETGHAKAVSIRL
                     VAQRVGVTPPSIYLHFADKDTLLDAVCARYFEKLDEEMQRAALDEPSTVEVLRAQGLA
                     YVRFAMQTPELYRIATMGEGRPGSDIDMTLNSSAFQHLRSSVEKLIEEGVYPPGDPTM
                     AALQLLTAAHGVASLLISKPYLPWGDVEEFADQTLSAACLGRIVLGLVEDDDATPQQT
                     VDWLLNRKPREGKS"
     gene            complement(1122..>1700)
                     /locus_tag="VGP27_01015"
     CDS             complement(1122..>1700)
                     /locus_tag="VGP27_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7849929.1"
                     /translation="LKALVLNVLSLTAAFGALVWIFQEGHLGALGTTSTGTLVTNMPV
                     LLFCIAFGLAMDYEVFLVSRIREYWLSSPGRTRADNDEAVARGLARTGRVVTAAALVM
                     SISFAALMAAQVAFMRMFGVGLTLAILVDATLVRTLLVPAFMHVLGRWNWWAPKPLAR
                     LHDRIGISESSDAPIPAAEKQVRAAVSVTDTG"
BASE COUNT          305 a          579 c          535 g          281 t
ORIGIN      
        1 ggaagcgcag ctcgccgccg ggccggacga gcgagaaaag ttgtcgcaga acactgtccg
       61 ggtcgtcgat cgagcagagc actagagagc acacgacggc gtcgaacgcc ttcccgtcgc
      121 catcgctgag gaacttctcg acggtgccgg tgctcaccgt gaccgggacg gacgctttcg
      181 ccgccgcttt tcgtgcctgc ggcgccagcc gtcgttcggg ttcgacggcg acgacttcgg
      241 tcacggtcgc cgggtagcac gcgaagttcg tgccggtacc tgcgcccact tccagcacgc
      301 ggccggtcag accggccagg ttctccgtcc gtagccgctg gatctcctcg gtttcgtggt
      361 tcgacaggaa ggtccacagt cgggcgaaga acggattgtc tgtggtgtcg tcggcgtcgg
      421 tcacgacttc ccctcccgcg gtttacgatt cagcaaccag tcgacggtct gctgtggtgt
      481 cgcatcgtcg tcctcgacga gcccgaggac tatccggccc aaacacgccg ccgacaacgt
      541 ctggtcggcg aattcctcca cgtcgcccca cggcagatac ggcttcgaaa tcagcagtga
      601 ggcaacgccg tgagcggctg tcagcagctg caacgcggcc atcgtcggat caccgggcgg
      661 gtagacaccc tcctcgatca acttctcgac cgagctacgc aggtgctgaa acgccgagct
      721 gttcagtgtc atgtcgatgt cgctgccggg cctgccctca cccatcgtgg cgatgcggta
      781 caactcgggc gtctgcatcg cgaaccggac atacgccagc ccctgcgccc gcagcacctc
      841 gaccgtcgac ggctcgtcaa gggcagcccg ctgcatctcc tcgtcgagct tctcgaagta
      901 ccgcgcacag accgcgtcca gcagcgtgtc cttatcggcg aagtgcaggt agatcgatgg
      961 cggtgtgacg ccgacacgtt gcgcgaccaa ccggatggag acggccttgg cgtgcccggt
     1021 ctccagcagc aaccgggtgg tggcgtccag gatttcgttc ctcagctgct cgccggagcc
     1081 acgcggcgca cgccgtcgct tgaggggctg tagggacatc gtcaaccagt gtccgtcacc
     1141 gagaccgcgg cccgcacctg tttctccgcc gcgggtatcg gggcgtcgga cgattcgctg
     1201 atgccaatgc gatcgtgcag ccgggccaac ggtttgggcg cccaccagtt ccagcggccc
     1261 agcacgtgca tgaacgcggg cacaagcagc gtgcgcacca gcgtcgcatc gacgaggatc
     1321 gccagcgtca ggccgacacc gaacatccgc atgaacgcga cctgggcggc catcaaggcg
     1381 gcgaacgaaa tcgacatcac cagcgccgcg gcggtgacca cccggccggt tcgggccagg
     1441 ccgcgggcaa cggcttcgtc gttgtcggcc cgcgtccgcc ccggtgaaga cagccaatac
     1501 tcccggattc ggctgacgag gaacacctcg taatccatgg ccaacccgaa tgcgatgcag
     1561 aacaacagca ccggcatgtt ggtgaccagt gttccggttg atgtcgtgcc cagcgcgccg
     1621 aggtggccct cctggaagat ccacaccaac gcaccgaatg cggccgtcag cgacaacacg
     1681 ttgagcacca aagctttcag
//
LOCUS       DASYLX010000040         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3183749,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000040 DASYLX010000000
VERSION     DASYLX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183749"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGP27_01020"
     CDS             complement(<1..438)
                     /locus_tag="VGP27_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HEV7849930.1"
                     /translation="MAGQLIVSISGIGEQTLPDVVAFCAQLEKRAVPSSFLVAPRLKG
                     GYRLDQDIPTVDWLASRRTRGDAIVLHGFDEAATKKRRSEFATLPAHEANLRLMGADR
                     VLEHLGLRTRLFAAPGWTVSQGTVTALPRNGFRLVAELSGITDL"
     gene            complement(506..1003)
                     /locus_tag="VGP27_01025"
     CDS             complement(506..1003)
                     /locus_tag="VGP27_01025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV7849931.1"
                     /translation="MTDPQLGDIALTTLDGRATTLAELAAGAALVVNVASRCGLTPQY
                     SALEKLAQDYGDRGLTVVGVPCNQFMGQEPGSAEEIQTFCSTTYGVTFPLLAKTDVNG
                     PDRHPLFAELTKTADATGEAGDVAWNFEKFLLAPGGEVVKRFRPRTEPDAPEVVEAIE
                     AVLPR"
     gene            complement(1044..1640)
                     /locus_tag="VGP27_01030"
     CDS             complement(1044..1640)
                     /locus_tag="VGP27_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7849932.1"
                     /translation="MSSRDTYHHGDLKATILGEAALLVAERGADGLSLRELARAAGVS
                     HAAPAHHFTDRRGLFTALATEGFRKLADALAGARPDFLDAALAYVRFAIDHPGHYAVM
                     FDKSLYDASDTELVAAETAAGAELAAGVGTLDDARAKDDPQAAALAAWSLVHGFSLLL
                     LNNAVDDGTDPMATVRRVAGMLFGTPSDDDQAARREPR"
     gene            complement(1698..>2031)
                     /locus_tag="VGP27_01035"
     CDS             complement(1698..>2031)
                     /locus_tag="VGP27_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV7849933.1"
                     /translation="ADENVYHYMKSYSPYENVGAKDYPPILAMTSLNDTRVYYVEPAK
                     WVAALRYMKTDGHPVLLKTEMSAGHGGISGRYERWKEAAFQYAWLLDTAKAHHEGGAY
                     EDNLLDRP"
BASE COUNT          326 a          677 c          673 g          355 t
ORIGIN      
        1 caggtcggtg atcccgctga gctcggctac cagccggaag ccgttgcggg gcagagcagt
       61 caccgtcccc tgcgacaccg tccaacccgg cgcggcgaac aaccgggtgc gcaggccgag
      121 gtgctcgagc acccggtcgg cacccatcag ccggagattc gcttcgtgtg cgggcagggt
      181 cgcgaactcg ctgcgtcgct tcttcgttgc ggcttcgtcg aagccgtgca ggacgatggc
      241 gtcgccgcgg gttcgacgcg aagccagcca atcgacggtc ggaatgtcct ggtcgagccg
      301 gtagccgccc ttgagccgcg gcgcgacgag gaacgaggac ggtaccgccc gcttttccag
      361 ttgtgcgcag aacgccacaa cgtcgggcag cgtctgctca ccgatgccgg agatcgagac
      421 gatcagttgt ccagccacac cgccattgtc gcaacgcgag gtgtcgcaac ggtttacgac
      481 actcttccag cagttgtcta ttccgctacc gcggcagcac cgcctcgatg gcctcgacca
      541 cctcaggggc gtccggttcg gtgcgcggcc ggaaccgctt gaccacttct ccacccggtg
      601 cgagcaggaa cttctcgaaa ttccacgcca cgtcgccggc ctcaccggtg gcgtcggcgg
      661 tcttcgtcag ctcggcgaac agcgggtgac ggtcgggccc gttgacatcg gtcttggcca
      721 gtaacgggaa cgtgacgccg taggtggtgg agcagaacgt ctggatctcc tcggcgctgc
      781 cgggctcctg acccatgaac tggttgcacg gcacgccgac cacggtcagc ccgcgatcgc
      841 cgtaatcctg tgcaagcttc tccagcgcgc tgtattgcgg tgtcaacccg cacctggagg
      901 cgacgttgac gaccagcgct gcgcctgcgg cgagctcggc cagcgtcgtg gccctaccgt
      961 caagtgtggt gagtgcgatg tcgccgagct gcgggtctgt catggcggga acactaccgg
     1021 ctacgtccct ctcgtcgaac tcctcaacgc ggctcgcgcc tcgccgcttg atcgtcgtcc
     1081 gacggtgttc cgaacagcat tccggccacc cggcggactg tggccatcgg gtcggtgccg
     1141 tcgtcgaccg cgttgttgag cagaagcagc gaaaaaccgt gcaccagaga ccatgccgcg
     1201 agtgcggccg cctgcggatc atccttcgcg cgcgcgtcgt cgagcgttcc gacaccggcg
     1261 gccagctcgg caccggcggc cgtctccgcg gcgaccagtt cggtgtcgga cgcgtcgtag
     1321 agcgacttgt cgaacatcac ggcgtagtgg ccgggatgat cgatggcgaa ccggacgtac
     1381 gcgagcgccg cgtcgaggaa gtccggccgc gcgcccgcca gcgcgtcggc cagcttgcgg
     1441 aacccctcgg tcgccaacgc ggtgaacagg ccacggcggt cggtgaagtg atgggcgggc
     1501 gcggcatggg agacgcccgc ggcacgggcc agctcacgca gcgagaggcc gtcggcgccg
     1561 cgctcggcca ccagaagcgc cgcctcgccg aggatggtgg ccttcagatc accgtggtgg
     1621 taggtatccc tgctgctcat gcaacccatc gtagcgcgac aacttgacag tgcctagatt
     1681 gttgctcagg gctgccgcta cggacggtcg agcaggttgt cttcgtaggc gccgccctcg
     1741 tggtgggcct tggcggtatc cagtagccag gcatactgga aagccgcctc cttccagcgc
     1801 tcgtagcgac cgctgatgcc accatggccc gcgctcatct cggtcttcaa cagcaccgga
     1861 tggccgtcgg tcttcatgta tcgcagtgcc gcaacccatt tcgcgggctc gacatagtaa
     1921 acccgggtgt cgttgagtga tgtcatcgcc aggatcggcg ggtagtcctt ggcaccgacg
     1981 ttctcatacg gcgagtagga cttcatgtag tggtagacgt tctcatcggc c
//
LOCUS       DASYLX010000041         6077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3403206,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000041 DASYLX010000000
VERSION     DASYLX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403206"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGP27_01040"
     CDS             complement(<1..633)
                     /locus_tag="VGP27_01040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849934.1"
                     /translation="MTGSGRVGEARVAIVTGASSGIGFGCATKLAGMGMAVLGVGRDA
                     DRLADLERAIGDPERVATISVDLTADDAPTRIVDLAVQRWGRVDFLVNNAGVGSPKPL
                     HETDDESLDYFLDLMLRAPFRLARDVIPHMQAGSAIINVTSTFAVVGGLRGGAYSAAK
                     GGLTSLTMHIACQYGPQGIRCNAVAPGVTLTPMVAQRLEDERFKKINTEMT"
     gene            complement(635..2164)
                     /locus_tag="VGP27_01045"
     CDS             complement(635..2164)
                     /locus_tag="VGP27_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7849935.1"
                     /translation="MPTLADSIAEAARLTPQRAVLVDGDVRLDCESLYAQANTLARAL
                     LAHMPTGSVVSFMLPNWHEAAVVYLAATLAGIAVNPILPSLRERELAFILEDVDSRML
                     FIPREFRQHDYLAMVDRVTAGMQSPPEVVVVRGQSGDRAAYAGLFEQECDWKELPALD
                     PAAVRMILYTSGTTGRPKGVLHSHDSIHALICQLRDHWLIDPGDTFLVPSPIAHIGGS
                     IYAFECPLLLGTTAVLMDRWDPDEAVTLMSSERCTHMAGATPFLEGLLAAAERAGTRL
                     PDLKVFICGGASVPPTLIRRAADYFSRAVVTRVYGSTEVPVTTVGSPTDSQHAADTDG
                     RPGIATVKLVAHETAPPGSGEIHARGPQMLVGYRDPEDERDAFDDEGYFRTGDLAEWV
                     DGDYLVVTGRAKDIIIRSGENIAPKEIEDILARHPDIVEISIVGLPDARTGERACAVI
                     VPQAVPGPDVDSLRCFLADYGVAKFKVPEQVVIWEMLPKNDAGKVLKHQIRAALTKAD
                     G"
     gene            complement(2169..2903)
                     /locus_tag="VGP27_01050"
     CDS             complement(2169..2903)
                     /locus_tag="VGP27_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7849936.1"
                     /translation="MTETSPVDHRYIQVARALRKDIVEGVYPVGSQLPTEHELCERFS
                     VSRYTVREALRRLREDNLVSSRPRTGTLVVPRSTSDAYDVMSINDLVAFATEARFAIE
                     SISMVTLDDDVAARTGLGGGEEWLAVRGFRQSEGADFALCRTEYYINRAFASVGRLLQ
                     RHTGPIFPLIEDLFGVSIVEVNQEIAAVLIPPELAQGLKIEPGSPALEVQRTYTTSDG
                     EVAQVTINTHPASRFRHSMTMRRVRG"
     gene            complement(2917..4545)
                     /locus_tag="VGP27_01055"
     CDS             complement(2917..4545)
                     /locus_tag="VGP27_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEV7849937.1"
                     /translation="MSTSRNDARYSADTPSTAEGWRFERLTAPSRLFGANGLRAGPDG
                     RVYIAQVTGSQISALDVGSGRLETISAKGGDIIAPDDVAFDSDGNLYATEVMDGRVSV
                     LGTDGRTRVLRDDLPCANGITVHQGRLFINECRDGGRLMELDRASGAQRILLENLPSP
                     NAMEVGPDGLLYYPLMTANEIWRVDPEGGEPQRVAGDLGVPDAVKFDPDGYIVSTQVA
                     SGQVLRIDPRSGEQKLLAQLSPGLDNLALVDGRVFVSNFTGEITEILAADARSGSEHA
                     GETRNVLPGGLNWPLDLAVGGDGRLYVADGTYFYAVAPDGSLQTVGMLFSPGYPGFLR
                     GVVSAGDGEFVVTTSGGQVARYWPAVGETDYLATDFDQLYGVAIGSGGEIAFAELGTG
                     RVHALRSGNVETLASGLHDPVGVAFDPEGHVLVTESGAGRVVKLTGSGADVLLDGLAR
                     PQGLLVRDGQLYVVDAGSREVISFDLSSKARTTIASGLPVGPPPGVEPKPLKGMPPFS
                     GPQGPFAGVAAGPDGSLYVSADADGSVLALRRTS"
     gene            4687..5532
                     /locus_tag="VGP27_01060"
     CDS             4687..5532
                     /locus_tag="VGP27_01060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849938.1"
                     /translation="MSDLLKLDGRIVVVSGAGGGGIGTTATRMAAEAGATVVAVSRSK
                     ENLDEHIAPLGAGGLAVIPVAADASTDDGIATVLDQARAADGDLYGLVNVAGGAEPAT
                     WMPSTRVTRDDWRALFTANLETAFFMSQAVAAEIRRNQNRGSIVSISSISGMNTAPFH
                     IGYGTAKAAVVAMTRTMAVELAQDDIRVNAVAPGVTATAASLTYTGDDPDRDRQAIAM
                     GRRGTPEEQAGAILFLLSDLSSYITGQTLLVDGGLNLKWTHLGSDNTSLFLKDETFRA
                     AIRRM"
     gene            5532..>6077
                     /locus_tag="VGP27_01065"
     CDS             5532..>6077
                     /locus_tag="VGP27_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7849939.1"
                     /translation="MTDIETNATAAEELSEPVTIGVDAYISADYARAERDKLWRKVWQ
                     QVGRVEEIPEVGSYLTYDVLDDSIIVVRSGPNEFKAHHNVCMHRGRRLLDTPDGAKNA
                     CGRARKSFVCGFHGWSYGLDGTCTHIREQDDWKGALTPRNTHLAPVQVDTWGGWLFVN
                     MDPDCEPLADYLFPAAKILDPF"
BASE COUNT         1053 a         2023 c         1985 g         1016 t
ORIGIN      
        1 cgtcatctcc gtgttgatct tcttgaaccg ctcgtcctcg aggcgctgag cgaccatcgg
       61 cgtcagcgtc acgcccggcg ccactgcgtt gcaacggatt ccctgcggcc cgtactggca
      121 ggcgatgtgc atggtcagcg atgtcagccc gcctttcgcc gccgagtagg cgccgccacg
      181 caggcctccg acgaccgcga acgtcgacgt gacgttgatg atcgccgagc ccgcctgcat
      241 atgcggtatc acgtctcgcg ccagccggaa cggcgcgcgc aacatcaaat cgaggaagta
      301 gtccaatgac tcgtcgtcgg tctcgtgcag cggcttgggg ctgccgacac ccgcgttgtt
      361 gacgaggaaa tcgacgcggc cccaacgctg tacggcgagg tccacgatgc gggtaggcgc
      421 atcgtcggcg gtgaggtcga cggatatggt tgcgaccctc tcgggatcgc cgatggcccg
      481 ctccaggtcg gcgagcctgt ctgcatcgcg gcctacaccg aggacggcca tgcccatccc
      541 cgcgagtttg gtcgcacacc cgaagccgat gccgctgctc gcgcccgtca cgatcgctac
      601 tcgtgcttca cccacccgcc cagaacctgt catatcaccc gtccgccttc gtcaatgctg
      661 cccggatctg atgcttcagt actttgcctg cgtcgttctt gggcaacatc tcccagatga
      721 ccacctgctc aggcactttg aacttcgcga cgccgtagtc ggcaaggaag caccggagac
      781 tgtcgacgtc ggggccgggg accgcttgcg gcacgatgac cgcacatgcc cgctcgccgg
      841 tcctggcatc cggtaaaccc acgatggata tctcgacgat gtcgggatgc cgggcgagga
      901 tgtcctcgat ttccttcggg gcgatgttct cgccgctacg gatgatgatg tccttggccc
      961 tgccggtcac gacgaggtag tcgccgtcca cccactccgc gagatcgccg gtgcggaaat
     1021 agccttcgtc gtcgaaagcg tcccgctcgt cctcgggatc gcggtagccg accaacatct
     1081 gtggaccgcg ggcatggatc tcgcccgacc cgggcggtgc ggtttcgtgg gcgaccagct
     1141 tgacggtagc gatgccgggg cgcccgtcgg tatcggcggc gtgctgtgag tcggtgggtg
     1201 aaccgaccgt cgtgacgggc acctccgtcg agccgtacac ccgtgtcacc accgcacggc
     1261 tgaaataatc tgcggcccgg cggatcagag tcggcggtac cgacgccccg ccgcagatga
     1321 agaccttgag atcaggcaac cgggtgccgg cgcgctcggc cgctgcgagc agcccctcga
     1381 gaaacggagt cgcgcccgcc atgtgagtgc agcgctccga ggacatcagc gtcactgcct
     1441 cgtcggggtc ccagcggtcc atcagcacgg ccgtggtgcc cagaagtagc ggacattcga
     1501 aggcgtagat cgagccgccg atgtgtgcga tcggcgacgg caccaggaac gtgtctccgg
     1561 ggtcgatcag ccagtggtcg cggagctggc agatcagcgc gtgaatcgag tcatggctgt
     1621 gcagaacgcc tttcgggcgg cccgtggtgc ccgaggtgta gaggatcatt cgcacagctg
     1681 ccggatcgag tgccggcaac tccttccagt cgcactcctg ctcgaacagg ccggcgtatg
     1741 cggcgcgatc acccgactgc ccgcgaacga cgacgacctc cggcggcgac tgcataccgg
     1801 ccgtcacacg gtccaccatg gcgagatagt cgtgctgccg aaattcacgc ggtatgaaca
     1861 acattcggct gtcgacgtct tcgaggatga acgccagctc gcgctcacgg agtgagggca
     1921 gtatcggatt gacggcgatt cccgccaacg ttgccgccag gtagacgact gcggcctcat
     1981 gccagttagg aagcatgaag gacaccacgg agccggtcgg catgtgcgcc agcagcgccc
     2041 gcgccagcgt gttggcctgc gcgtagagcg attcgcagtc gagccggacg tcaccgtcga
     2101 ccagcacggc ccgttgcggt gtgagccgcg cggcttcggc tatcgaatcg gccagtgtgg
     2161 gcatgtcact agcctctgac ccgccgcatg gtcatcgagt ggcggaaccg ggacgcggga
     2221 tgggtgttga tcgtgacctg ggcaacctcg ccgtccgacg tcgtgtaggt gcgttgcact
     2281 tccagcgcgg gcgaccccgg ctcgatcttg agtccctggg cgagttcagg cgggatcagc
     2341 acagccgcga tctcctggtt cacctcgacg atgctgacgc cgaacaggtc ttcgatcagg
     2401 gggaagatcg gacccgtgtg gcgttgcaga agcctgccga ccgacgcgaa cgcacggttg
     2461 atgtagtact cggttcggca cagggcgaag tcggcgccct cggactgcct gaagccgcgg
     2521 acggccagcc actcctcgcc gccgccgagg ccggtccgcg cggccacatc gtcgtcgagc
     2581 gtcaccatgc tgatcgactc gatcgcgaag cgggcctcgg tggcaaaggc caccagatcg
     2641 ttgatcgaca tgacgtcgta ggcatccgac gtcgaccgcg gcacgacgag cgttccggtg
     2701 cgcggacgcg acgacaccag gttgtcctca cgcaacctgc gcagcgcctc ccgcactgtg
     2761 tagcggctga ccgagaaacg ttcgcacagt tcgtgttcgg tgggtagctg cgatccgacc
     2821 gggtacacgc cctccacgat gtccttgcgc agcgcgcgcg ccacctggat gtagcgatga
     2881 tcgacggggg atgtctcggt cattcgtgtc tgcctgtcag gaggtacggc gcaacgccag
     2941 cacgctgccg tccgcgtcgg ccgaaacata gagggagcca tcgggtcccg ccgcgacgcc
     3001 ggcgaacgga ccctgcggcc cggagaacgg cggcatgccc ttgagcggtt tcggttcgac
     3061 tccgggaggg ggacccacgg gcaggcccga ggcgatggtg gtgcgcgcct tgctgctcag
     3121 gtcgaacgag atgacctccc gcgagcctgc gtcgacgacg tagagttgac cgtcgcgcac
     3181 cagtaggccc tgcggtcttg ccagcccgtc cagcaggacg tcagcacccg atccggtcag
     3241 cttcacgaca cgaccggcgc ccgactcggt gaccagcacg tggccctccg ggtcgaacgc
     3301 gacgccgacc ggatcgtgca gacccgatgc gagtgtctcg acgttgcccg aacgcagggc
     3361 atgcacccgg ccggtgccga gttcggcaaa tgcgatttcc ccgcccgatc cgatcgccac
     3421 gccgtaaagc tggtcgaaat cagtggcgag atagtcggtt tcacctaccg ccggccagta
     3481 tcgcgcgacc tggccgcccg aagtggtcac gacgaactca ccgtcaccgg cggacaccac
     3541 accacgcagg aatcccggat agcccggcga gaacaacatt ccgaccgtct gcagtgagcc
     3601 gtcgggcgcc accgcgtaga agtaggtgcc gtcggcgaca tacagtcggc cgtcgccgcc
     3661 gaccgcgaga tccagcggcc agttcagccc acccgggagc acatttctcg tttcgccggc
     3721 atgctcgctt ccgctcctgg cgtccgcagc gaggatttcg gtgatctcac cggtgaagtt
     3781 cgagacgaac acccggccgt cgacgagtgc gaggttgtcc aggccggggg acagctgagc
     3841 cagcagcttc tgttcgccgc tgcgcgggtc gattcgaagc acctgcccgc tggcaacctg
     3901 ggtcgacacg atgtagccgt ccgggtcgaa cttcaccgcg tccggcacac caaggtcacc
     3961 ggcaacccgt tgcggctcac cgccttcggg atctacccgc catatctcgt tggcggtcat
     4021 cagcgggtag tacagcagcc cgtcggggcc gacttccatg gcgttgggcg acggcaggtt
     4081 ctccagaaga atccgctgcg cgccgctggc ccggtcgagt tccatcaacc gtccgccgtc
     4141 gcggcattcg ttgatgaaca gccgcccctg atgcacggtg atgccgttcg cgcaggggag
     4201 gtcatcgcgg agcacccggg tccggccgtc cgtaccgagc acgctcaccc ggccgtccat
     4261 cacctcggtg gcatagaggt tgccgtctga atcgaaggcc acgtcgtccg gcgcgatgat
     4321 gtccccgccc ttggcgctga tggtctcgag cctgccgctg ccgacatcca gtgcgctgat
     4381 ctggctgccg gtcacctgcg cgatatacac gcggccgtcc ggaccggcac gcaggccgtt
     4441 ggcgccgaac agccggctcg gtgcggtcag ccgttcgaac cgccagccct cggcggtgct
     4501 cggggtgtcc gcagagtaac gggcgtcgtt gcgcgacgtg ctcatggcct cggacgttat
     4561 caagccggaa tagtccagac aatagctcca atgagagttc tgcagggcct tgacgaccgg
     4621 attatggctg cggaatagtg ttctgcaata tgcagagcat cgtatgttgt ccggacaaat
     4681 agcctcatga gtgacctttt gaagctcgac ggccgcatcg tggtggtctc cggtgccggc
     4741 ggtggaggaa tcggtacgac ggcgacgcgg atggccgccg aggcaggcgc aaccgtcgtc
     4801 gcggtcagcc ggtccaagga gaatctcgac gagcacatcg cgccgctggg tgcgggcggg
     4861 cttgcggtga ttccggtggc ggccgacgcg tcaaccgacg acggcatcgc gacggttctc
     4921 gatcaggcgc gcgctgccga cggcgacctc tacggcctgg tgaacgtcgc aggcggcgca
     4981 gagcccgcca cctggatgcc gtcgacgcga gtgaccagag acgactggcg cgccttgttc
     5041 accgcgaacc tcgagaccgc gttcttcatg agccaggcgg tggcggccga gatccggcgc
     5101 aaccagaacc gcggttcgat cgtgtcgatc tcatcgatca gcggaatgaa caccgccccg
     5161 ttccacatcg gctacggaac cgccaaggcc gcggtggtgg cgatgacgcg gacgatggcg
     5221 gtcgaactgg cgcaggacga cattcgggtc aacgcagtgg ctccaggcgt gacggcgacg
     5281 gccgcgtctc tcacctacac cggtgacgat ccggaccgcg accgtcaggc catcgcgatg
     5341 ggacggcgcg gcacaccgga ggagcaggcg ggcgccatcc tgttcctgct ttccgacctg
     5401 tcgagctaca tcaccggaca gacgctgctc gtcgacggcg gcctgaacct gaagtggacg
     5461 catctcggat ccgacaacac gtctctgttc ctcaaggacg aaacctttcg agcggcgata
     5521 aggaggatgt agtgaccgac atcgaaacga atgcgacagc cgctgaggag ctctcagagc
     5581 cggtgaccat cggcgtcgat gcatacatct cggcggacta tgcccgcgcc gagcgggaca
     5641 agctgtggcg caaggtatgg caacaggtgg gccgcgtcga ggagatcccg gaagtcggca
     5701 gctatctcac ctacgacgtc ctcgacgact cgatcatcgt cgtacgtagc gggccgaacg
     5761 aattcaaggc gcatcacaac gtctgcatgc accgcggccg gcgccttctc gacacccccg
     5821 acggcgccaa gaacgcctgc ggccgagcac gcaagtcctt cgtttgcggt ttccacggct
     5881 ggtcctacgg gcttgacggc acctgcaccc acatccgcga gcaggacgat tggaagggtg
     5941 cgctgacccc gcggaacacc cacctcgccc cggtgcaggt cgacacctgg ggcggctggc
     6001 tgttcgtcaa catggacccc gactgcgaac cgctggccga ctacctgttt ccggcggcga
     6061 agatcctcga tccgttc
//
LOCUS       DASYLX010000042         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3512941,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000042 DASYLX010000000
VERSION     DASYLX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512941"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGP27_01070"
     CDS             <1..315
                     /locus_tag="VGP27_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849940.1"
                     /translation="SGWIGRRMVALFLEAGLEDVETHPKTLVCSDLDVADRVFSFFAT
                     ADGLVDAATIGADDVERWKNELRAADAAGRFFTSYTGFLVSKTRPLRDASRPTGLRGL
                     NV"
     gene            complement(295..801)
                     /locus_tag="VGP27_01075"
     CDS             complement(295..801)
                     /locus_tag="VGP27_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7849941.1"
                     /translation="MSNDSAAAIRQERRNWTAKHRAIYLQSGGTQGHIMDISEVGGHA
                     FTTHCLIRVAGRKSGKTRIVPLIYGDIGGEVAIVASKGGADTHPDWYLNLRDSEHVDV
                     QIATQAFRATWREPEDVERHRIWDFMVSVYPPYLTYQRSTTRHIPVVALEPVETIEIF
                     RESDVQSP"
     gene            817..1248
                     /locus_tag="VGP27_01080"
     CDS             817..1248
                     /locus_tag="VGP27_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7849942.1"
                     /translation="MTEGTAPAITGIHHFSVTVCDIETSAAWYQRVFRAQRVPMTFPH
                     YDREDTGYGVLLLEPGSGLAIGLHTNTGNDGAQFDEARTGLDHVGFNVDGREELERWA
                     AWLGELGIEHSGIRTGDEPFPYATLVFRDPDNIQLELFAVV"
     gene            complement(1263..2846)
                     /locus_tag="VGP27_01085"
     CDS             complement(1263..2846)
                     /locus_tag="VGP27_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV7849943.1"
                     /translation="MSELAKPARTQAKSGFRFPGFAQLQRLGKSLMLPIAVLPAAGIL
                     LRLGQPDLLGRIEAPVIGPFFKAMTAAGDALFTNLPLLFAVGVAIGFARKADGSTALA
                     AVVGYLVVQAVFKTMSPIVLAGEVDKAGEQAQINYSVFAGIVVGLVTAWLFDRYHTIQ
                     LPSYLGFFGGRRFVPIVVSLACLFLAFAMSYFYPIFDAGLTALGEFIGGAGALGAFVY
                     GFANRMLIPLGLHHIPNSYIWFLYGDYQPPGGAPVTGELTRFAAGDPTAGILTSGFYP
                     ILMFGLPAAALAMIHVANKKQRKVAVGILAAAGLTAFLTGVTEPLEFAFMFVAFPLYL
                     IHAVLTGLSLAIAYLLDIHLGFSFSAGLIDLLLYGTAPAAKNIPLLIAMGVVFFAVYY
                     LLFRFAITRWNLRTPGREPEDEFEAEEEANLGEGADSATTVSTARSGAATATVAAPVA
                     ADSKAEQVISAFGGRDNLVNVDACITRLRVEVNDKDKVDQARLKRLGAAGVVEVGNSV
                     QAVFGTDAEALKSDINDIL"
BASE COUNT          530 a          934 c          974 g          505 t
ORIGIN      
        1 tcgggatgga tcggccgccg gatggtcgcg ctgttcctcg aagcaggcct cgaggacgtc
       61 gagacccatc cgaagacact ggtatgcagc gacctcgacg tcgccgaccg ggtgttcagc
      121 ttcttcgcga ccgccgacgg cctcgtcgac gccgcaacca tcggcgcaga cgacgtcgag
      181 cgctggaaaa acgaattgcg ggccgctgac gcggcaggcc ggttcttcac ctcgtacacc
      241 ggcttcctgg tgtccaaaac ccgcccgctg cgagatgcct cgcgcccaac tggtctacgg
      301 ggactgaacg tctgactccc ggaagatctc aatggtttcg acgggctcga gcgcgacgac
      361 gggaatgtgc cgtgtcgtcg acctctggta tgtcagatac ggtgggtaaa ccgagaccat
      421 gaagtcccag atcctgtgtc gctcaacgtc ttcgggttca cgccacgttg cccggaatgc
      481 ctgggtggcg atctgcacgt cgacgtgctc gctgtcgcgg aggttcaggt accaatccgg
      541 gtgcgtgtcg gcgccgccct tcgacgcgac gatcgccacc tcgccgccga tgtcaccgta
      601 gatgagtgga acgatgcgcg tctttcccga cttgcgcccc gcaaccctga tcaggcagtg
      661 ggtggtgaag gcatggccgc cgacctcgga gatgtccatg atgtggccct gcgtgccacc
      721 cgattgcagg tagatggcgc ggtgtttggc cgtccagttg cggcgctctt gcctgatggc
      781 cgccgccgag tcattgctca tggcaccatc ttgcacgtga ccgagggaac cgccccagcg
      841 attactggga tccatcattt ctccgtgacg gtctgcgaca tcgaaaccag cgcggcttgg
      901 tatcagcggg tgtttcgcgc gcaacgggtg ccgatgacgt ttccgcatta cgatcgcgaa
      961 gacaccggtt atggggtgtt gctcctcgag ccgggctccg gcctggcgat cgggctgcat
     1021 acgaacaccg gcaacgacgg cgctcagttc gacgaggctc gaacggggct cgaccatgtg
     1081 ggtttcaacg tggatggtcg cgaggagttg gagcggtggg ctgcgtggct cggcgaactc
     1141 ggcatcgagc actccggcat caggaccggc gacgagccat ttccctacgc gacactggtt
     1201 ttccgcgacc ctgacaacat ccagttggag cttttcgcgg tcgtgtaatc gggctcagag
     1261 tcctagagaa tgtcgttgat atcgctcttc aaagcctcgg cgtcggttcc gaagaccgcc
     1321 tgcacgctgt tgccgacctc gaccacaccg gcagccccga ggcgcttcag ccgcgcctga
     1381 tcgaccttgt ccttgtcgtt gacctcgacg cgaagccggg tgatgcaggc gtccacgttc
     1441 acaaggttgt cccggccgcc gaacgccgag atcacctgtt ctgccttgct gtcggctgcg
     1501 accggcgcgg ccaccgtcgc ggtggccgca cccgaccggg ctgtgctcac ggtggtggcg
     1561 gagtcggcgc cctcgccgag gttggcctcc tcctcggcct cgaactcgtc ctccggttcc
     1621 cggcccggcg tccgcaggtt ccaccgtgtg atcgcgaacc ggaacagcag gtagtagacg
     1681 gcgaagaaca ccacacccat cgcgatcagc agcgggatgt ttttcgccgc cggggccgtg
     1741 ccgtagagca gcaggtcgat gagacccgcc gagaacgaga agcccaggtg gatgtcgagc
     1801 aagtaggcga tcgccagcga cagtcccgtc agcacagcgt gaatgagata cagggggaac
     1861 gccacgaaca tgaacgcgaa ctcaagcggt tcggtcaccc cagtcaggaa cgcggtcagg
     1921 cccgccgccg caaggatgcc gacagccacc ttgcgctgct tcttgttcgc cacgtggatc
     1981 atggccagcg cggctgcggg cagcccgaac atcaggatcg gatagaagcc cgacgtcagg
     2041 atgcccgcgg tggggtcgcc cgcggcgaac cgggtgagct caccggtcac gggcgcgccg
     2101 ccgggcggtt ggtagtcgcc gtagaggaac cagatgtagg agttcgggat gtggtgcaac
     2161 ccgagcggaa tcagcatgcg gttggcgaac ccgtagacga acgcgccgag agcacctgcg
     2221 ccgccgatga actcgccgag cgccgtcagg ccggcgtcga agatcggata aaagtagctc
     2281 atggcgaagg cgaggaacag gcacgccagc gacaccacga tcgggacgaa ccgcctgccg
     2341 ccgaagaatc cgagatagga cggcagttgg atggtgtggt agcggtcgaa gagccacgcg
     2401 gttaccaggc cgaccacgat gccggcgaag acgctgtagt tgatctgggc ctgctcgccc
     2461 gccttgtcga cctcgccggc gaggacgatc ggcgacatcg tcttgaacac ggcttgcacc
     2521 acgaggtagc cgacgacggc ggcgagtgcg gtcgagccgt cggccttcct ggcgaatccg
     2581 atggcgacgc cgacggcgaa cagcagcgga aggttggtga acagggcatc gcctgctgcg
     2641 gtcatcgcct tgaagaacgg gccgatgacc ggtgcctcga tcctgcccag caggtcgggc
     2701 tggcccaacc ggagcaggat gccagccgcc gggagcaccg cgatcggcaa catgaggctc
     2761 ttgcccagcc gctgcagctg cgcgaagccg gggaaccgga aacctgactt tgcctgtgtt
     2821 cgcgcgggtt tcgctagctc gctcatgccg gtcaccctct ctgcgtgcaa acgtttctgc
     2881 cgccgaagct gtgcggaagc ctagaccaga acgagtgaac ttgagaccgt gttgcggcga
     2941 cac
//
LOCUS       DASYLX010000043         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2854785,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000043 DASYLX010000000
VERSION     DASYLX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854785"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGP27_01090"
     CDS             <1..394
                     /locus_tag="VGP27_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7849944.1"
                     /translation="LGRGLTLNDLDGQDVVMYSPVQARYFNELLISTFTIAGATPRYV
                     QTVTQVHTMLVLVRSGIGIALVPASAATLHPEGVVFRSIGAFRERPVELDAVWRGDST
                     NPALLRLLRDVLPQREWTTDDLVEDVLG"
     gene            complement(427..702)
                     /locus_tag="VGP27_01095"
     CDS             complement(427..702)
                     /locus_tag="VGP27_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849945.1"
                     /translation="MSSEHVTEQVPLAEIQEGDTIQDPESGQWFTVTRTADGTTSAVH
                     GGSGGDTTVVEAYRVYYGDERRGEQIDSRCIIDSRSTKGLVTRQVRE"
     gene            complement(837..2405)
                     /locus_tag="VGP27_01100"
     CDS             complement(837..2405)
                     /locus_tag="VGP27_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7849946.1"
                     /translation="MPETAYASCGDLSLAYQVFGVGPIELVFVGPMVSHVELLWTLPE
                     FKSFFDQLATFCRVVLFDKAGLGLSDPIARVRTLDERAAEIEAVMDAVGFTRPAIFGM
                     SEGGPSSILFAAKRPERTRALILYGTYTFMSVGWDDLDCEPSELMLKQGIQISDDVSE
                     KGKYAWSEEQVARILKMARAARFRWGSGETAKYMLPSVKSIRQLAMFERMVASPGMVR
                     ATMESTFRIDVRPILPTISAPTLVIHARDEPMPVQLGRYIADHIPGARMLEVEGVDHA
                     PFLTEPARILSEIEELLTGGHAPPSASHRALRTVLFTDIVASTRSAAAMGDERWRSVL
                     QRFGEITAEMVERFGGTVVKSTGDGHLMTFDGPTHAIRCAEDLRADAELLGIDIRAGI
                     HTGECELLGDDIGGLAVHIAARILSHSGAGDIVVSSTVRDLVVGSGTVFENRGSVELR
                     GVPGSWDLLAVDRVGAQPASAEAKLASAPTPGRGADMRRTDRAVETMARRTPWILRGV
                     ARLSPVTGRSTVRS"
     gene            complement(2469..>3249)
                     /locus_tag="VGP27_01105"
     CDS             complement(2469..>3249)
                     /locus_tag="VGP27_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV7849947.1"
                     /translation="SALPNLGAVVNFGVGYDTTDVDAAAERGVAVSNTPDVLTDCVAD
                     TAVGLMIDVMRQFSAADRYVRAGKWPTDGMYPLTRQVSNAQVGIIGLGRIGGAIAKRL
                     SAFGCTISYHNRHRVADCDYTYVPSPEELARRVDVLVVAAAGGSGTRRLVNREVIDAL
                     GADGYLVNIARGSVVDEEALVEALEGGRLAGAGLDVFAAEPDVPEALLAMDNVVVLPH
                     VGSGTVQTRAAMEALTLRNLERFLSSGELVTPVPMPVKASG"
BASE COUNT          546 a         1114 c         1022 g          567 t
ORIGIN      
        1 gctggggcga ggactgacgt tgaacgacct cgacggccag gacgtggtga tgtactcgcc
       61 ggtgcaggca cgctatttca acgagttgct catcagcacg ttcacgatcg ccggcgcgac
      121 gccgcgctac gtgcagaccg tgacgcaggt gcacacgatg ctggtgctgg tgcgttcggg
      181 catcggcatc gcgctggtgc cggcatccgc cgcgacgctg caccccgaag gggtggtgtt
      241 ccgttcgatc ggggcgtttc gggagcggcc ggtcgagctc gacgcggtgt ggcgtggcga
      301 cagcaccaac cccgcactgc ttcgtctact gagagacgtt ctgccgcagc gggaatggac
      361 gaccgacgat ctcgtcgagg atgtgctcgg ctaagcgcgc tctgcgccgg ccgtgggcga
      421 tcgcctctac tctcgcacct ggcgagtgac gaggcctttc gtggatcgcg agtcgatgat
      481 gcatcgggaa tcgatctgct cacctcggcg ttcgtcgccg tagtagacgc gatatgcctc
      541 gacgacggtc gtgtcaccgc ctgagccccc gtggacggcg ctcgtcgtgc cgtctgccgt
      601 tcgggtgacg gtgaaccact gaccggactc ggggtcttgg atcgtgtcac cctcttggat
      661 ctcggccagc ggcacctgct ctgttacgtg ttccgaactc atcaccctcc cggatcgtcg
      721 cggtggccgg ctgcatccag cgccaccccg acgattcttc taccaacggc gcgcgtcaat
      781 ccccgttttg gcagagcgag ccgaccatgg gctcgtcgta gagactccac ccactcctag
      841 ctgcgaacgg tgctgcggcc cgtcaccggc gacagtcggg ccactccgcg caggatccac
      901 ggggtccgcc tggccatcgt ctccacggcg cgatccgttc ggcgcatgtc ggcgccccgg
      961 ccgggggtgg gcgcggacgc aagcttcgct tctgccgaag ccggttgcgc gcctacacga
     1021 tcgaccgcca agagatccca gctgcctggc accccgcgca actcaacact gccgcggttc
     1081 tcgaagacgg tgccggagcc gacgacaagg tcgcgcacgg tgctcgacac gacgatgtca
     1141 cccgcgcccg agtggctcag tatccgcgcc gcgatgtgaa ctgcaaggcc gccgatgtcg
     1201 tctcccagca gctcgcactc tccggtgtgg ataccggcac gaatatcgat gcccagtaat
     1261 tcagcgtctg cccgcaggtc ttctgcgcat cgaatcgcat gggtcggccc gtcgaaggtc
     1321 atgaggtggc cgtcccctgt gcttttcacc accgtgccgc cgaagcgttc aaccatctcg
     1381 gcggtgatct caccaaagcg ctgcaagacc gagcgccacc gctcatctcc catcgctgct
     1441 gcggatcggg tcgacgcgac gatgtcggtg aacagtacgg tgcgcagtgc gcgatgtgac
     1501 gccgacggcg gagcatgacc gccggtgagc aactcctcga tctcgctcag gatcctggcc
     1561 ggctccgtca agaacggcgc gtggtccacc ccctcgactt cgagcatccg cgcgccgggg
     1621 atgtggtcgg cgatgtagcg gcccagttgc accggcatcg gctcgtcccg agcgtggatg
     1681 accagtgtcg gcgcggagat cgtcggcagg atcggccgca cgtcgatacg gaatgtcgat
     1741 tccattgtcg ctcggaccat tccggggctg gcgaccatgc gctcgaacat ggcgagctgc
     1801 cggatcgatt tcaccgacgg caacatgtac ttcgcggttt cgccgctgcc ccaacggaag
     1861 cgggccgcac gtgccatttt caggatacgc gcgacctgct cttccgacca tgcgtacttg
     1921 cccttctcgg acacgtcgtc gctgatctgg atcccttgtt tcaacatcag ctcggacggt
     1981 tcgcaatcga ggtcatccca cccgacggac atgaaggtgt acgtgccgta gaggatcagc
     2041 gcgcgcgttc gctcggggcg tttggccgcg aacagaatgg aggacggacc accctcgctc
     2101 atgccgaaga tggcaggtct ggtgaagccg acggcgtcca tgacggcttc gatctccgcg
     2161 gctcgttcgt cgagcgtccg aactcgcgca atcgggtcgg acaatccgag tcccgccttg
     2221 tcgaacagga cgacacgaca aaaggtggcc agctggtcga agaacgattt gaactccggc
     2281 aaggtccaca gcagctccac gtggctgacc atcggcccga cgaagacgag ttcgatcggc
     2341 ccgaccccga aaacctgata ggccaggctc aaatcgccgc atgaggcgta ggccgtctcc
     2401 ggcatgggct gagcgtaatt gacgccagcg ccggaaggtc acgatcagca gtcgcagaag
     2461 gaaaacgcct atccagatgc tttcaccggc atcggtaccg gggtcaccag ttcgcccgac
     2521 gaaagaaagc gctctaggtt gcgcagcgtg agcgcctcca tcgccgcgcg cgtctgcacc
     2581 gttccgctgc ccacgtgcgg caggactacg acgttgtcca tcgccagcag cgcctccggc
     2641 acgtcgggct cagcggcgaa gacgtccagg cctgcgccgg cgagccgacc accctcgagt
     2701 gcctcgacca acgcctcctc gtcgacgacg ctgccccgcg cgatgttcac gagatatccg
     2761 tcggctccca gcgcatcgat cacctcccga ttgaccagcc tccgggttcc cgacccgcct
     2821 gcggccgcga ccaccagaac gtcgacccgc cgggcgagct cctccggcga cggcacatag
     2881 gtgtaatcgc agtcggcgac ccggtggcga ttgtggtagg agatcgtgca tccaaaggcg
     2941 ctgagccgct tggcgattgc gccgccgatt cgcccgagcc cgatgatccc cacctgcgcg
     3001 ttactgactt ggcgcgtcaa cgggtacatg ccgtcggtgg gccacttgcc cgcccgcacg
     3061 taccggtccg ccgccgagaa ctgccgcatc acatcgatca tcagcccgac ggcggtatcg
     3121 gccacgcagt cggtcagtac gtcgggcgtg ttgctgacgg cgacccctcg ttccgccgca
     3181 gcgtcgacat ccgtcgtgtc gtagccgacc ccgaagttca ccaccgcgcc gaggttcggc
     3241 agcgccgac
//
LOCUS       DASYLX010000044         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2744835,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000044 DASYLX010000000
VERSION     DASYLX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744835"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGP27_01110"
     CDS             <1..280
                     /locus_tag="VGP27_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEV7849948.1"
                     /translation="VTNRERLGQARAALLAALFDREPPTPPTAAIVSLLHAVDGLGAL
                     LSLNDRGWRWVHMRASEIASGSWIDEYETGLPEMNLAVTASAMRSALT"
     gene            complement(272..1426)
                     /locus_tag="VGP27_01115"
     CDS             complement(272..1426)
                     /locus_tag="VGP27_01115"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV7849949.1"
                     /translation="MSENSRWQGLATKAIHAGFRPDPQTGAVNAPIYASSTFAQDGVG
                     GLRGGFEYARTGNPTRAALEASLAAVEEATFGRAFSSGMAATDCALRAVLRPGDHIVM
                     PDDAYGGTFRLIDKVFTQWGIDYTAVALSDLDAVRDAITPATKLIVVETPTNPLLSIA
                     DIGAIARIAAPSNVKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALL
                     TSDAQLDEAFGFLQNGAGAVPGPFDAYLTMRGLKTLVLRMQRHSDNAAVVAEFLENHP
                     AIETVLYPGLPSHPGHLIAAKQMTGFGGMVSVRLRGGVDAARDFCSRTEVFILAESLG
                     GVESLIEHPGAMTHASTAGSQLEVPADLVRLSVGIEDAADLLADLEHALS"
     gene            1473..2312
                     /locus_tag="VGP27_01120"
     CDS             1473..2312
                     /locus_tag="VGP27_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7849950.1"
                     /translation="MEQFRRGDLVFEVIDTGPADGPVVVLLHGFPETNAMYQPVIDRL
                     TAKGYRCLAPLQRGYSPGARPKRRRDYRADDLVDDLLTLIDASGAQRVHLVGHDWGAA
                     VAWYVAQAFPERLLTLTALSVPHPAAFLKAVRTSRQALASWYMLFFQLPWLPERLLLS
                     RRATAGFVESKPAGSTELAEAEVRAMREPGALTARLNWYRAMGRSDPRKLARRVTVAT
                     MYVWSDDDIALKEKGARLCADYVNAEYRFETFEGVSHWIVDERPDEVSDLLLDWLAAH
                     PTA"
     gene            complement(2277..3743)
                     /locus_tag="VGP27_01125"
     CDS             complement(2277..3743)
                     /locus_tag="VGP27_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7849951.1"
                     /translation="MESCDVCVVGAGVAGLNALFVASQYLSRDQKLILVDRRARVGGM
                     WVDTYPYVRLHQPHPMFTAGDIKWTLGRSRSYLATQGEVLDHFAHCLNVIKARVRVDE
                     LFGWEVESQDETAGIVRVTCRSSDGRRLVIETKRLIKAYGFQCKPNDPLELSSERVQS
                     VSPNFCDVRTDDIGASDDPVWIIGGGKTAMDTAHALITSYPGREVNLVAGSGTYFLCR
                     DQVLPTGLARWWRGKSFNSLAMDVAHRFDGTNESAVNAWFRDTYGLHLTPQTGNFLLG
                     LMSDSESKTIAAGLNDVVMDHLEDVVDRNDSTDLVLRSGSTKSIQPGSWVVNCTGYLN
                     HDDRPYEPYVSRSGAVLSINPRSVTLQLSTFMAYFMTHLMMLGKIRDVPLYELDAMEL
                     RKKANSAFTLTLFALGLHNSSIIFEAAPNKVFSQCGIDFNNWLPLPRRTLATARFMLT
                     HRRERERYRRTLNVVRERFDVRCGLLDLGSRVRSQPIQ"
BASE COUNT          678 a         1216 c         1231 g          655 t
ORIGIN      
        1 ggtgaccaac cgtgaacggc tggggcaggc tcgcgcagcg ctgttggcgg ccctgttcga
       61 cagggaacca ccgacaccgc cgacagcggc gatcgtgtcg ctgctgcatg ccgtcgacgg
      121 cctcggcgcg ctgctcagcc tcaacgaccg cggctggcgg tgggtgcaca tgcgcgccag
      181 cgagatcgcc agcggcagtt ggatcgacga gtacgagacg gggctgcccg aaatgaacct
      241 cgcagtcacc gcatcggcga tgcgcagcgc gttaacttag tgcgtgctcc aggtcggcaa
      301 gcaggtcagc ggcgtcctcg atgccgacag aaagccgaac cagatcggcc ggcacttcca
      361 gctgcgaacc tgccgtcgac gcgtgcgtca tcgcgccggg atgctcgatc agcgactcca
      421 ccccgccgag tgattctgca aggatgaaaa cctctgtccg cgaacagaag tcacgtgcag
      481 cgtcgactcc accgcgcagc cgtaccgaca ccatgccgcc gaagcccgtc atctgcttgg
      541 cggcgatgag atggccggga tggctcggca ggccgggata gagcacggtc tcgatcgcgg
      601 gatggttctc caggaactcg gcgaccactg cggcgttgtc gctgtgccgc tgcatccgca
      661 gtaccagcgt cttgagcccg cgcatggtca gataggcatc gaacgggccc ggcaccgcac
      721 ccgcgccgtt ctgcaggaat ccgaaagcct cgtcgagttg agcgtcgctg gtcagcagag
      781 cgccaccgac gacgtcggaa tgccccccga tgtacttggt cgtcgagtgc aacacgatgt
      841 ctgcgcccag caacagaggc tgctgcagcg caggcgaggc aaacgtgttg tcgaccaaca
      901 cctttacgtt cgacggggca gcgatacggg cgatggcgcc gatgtcggcg atggacaaca
      961 gcgggttcgt cggcgtctcg accacgatca gtttggtcgc aggtgtgatg gcgtcccgaa
     1021 cggcgtcgag gtcggacagc gccaccgccg tgtagtcgat accccactgg gtgaagacct
     1081 tgtcgatgag cctgaaggtg ccgccatagg cgtcgtcggg catcacgatg tgatcgccgg
     1141 gacgcagcac cgcccgcaac gcgcagtcgg ttgcggccat ccccgagctg aacgcgcgcc
     1201 cgaaggtcgc ctcctccacc gcggcgagcg atgcctccag cgctgcgcgg gtcggattgc
     1261 cggtgcgggc gtactcgaac ccgccccgca gcccgcccac gccgtcctgg gcgaatgtcg
     1321 agctggcgta gatcggggcg ttgacggcgc cggtctgcgg gtcagggcgg aaacccgcgt
     1381 ggatggcctt ggtggccaga ccctgccacc tgctgttctc actcatcacc gcccagcgta
     1441 accaggcctg cccggcgggt taccgtcagc cgatggaaca gttccgccgc ggcgacctcg
     1501 tcttcgaggt aatcgatacc ggacccgccg acgggccggt ggtcgttctt ctgcatggct
     1561 tccccgagac caacgcgatg taccagccgg tcatcgaccg gctgaccgcc aagggttacc
     1621 gctgtctggc gccgctgcag cgcggctact cgccgggcgc gcggccgaag cggagacgcg
     1681 actaccgcgc cgacgatctg gtagacgatc tgctcacgct gatcgatgcc agcggtgcgc
     1741 aacgcgtgca tctcgtcggc catgactggg gcgcagccgt cgcctggtat gtcgcacaag
     1801 cgtttcccga gcgcctgctc acgctgacgg cgctctcggt tccgcacccg gccgcgtttc
     1861 tgaaggccgt caggaccagt cgccaggcgc tggcgtcgtg gtacatgctt ttcttccagc
     1921 tgccgtggct cccggaacgg cttctgctga gcagacgcgc cacggccggc ttcgtcgaat
     1981 cgaagcctgc gggttcgacg gagttggccg aagccgaagt cagggcgatg cgggagcccg
     2041 gtgcgttgac cgcccgcctg aactggtacc gcgcgatggg gcgcagtgac ccacggaagt
     2101 tagccagaag ggtcaccgtg gcgacgatgt acgtctggag cgacgatgac atcgctctga
     2161 aggagaaggg tgcgcggctc tgcgccgact acgtgaacgc cgagtaccgc ttcgagacct
     2221 tcgagggcgt ttcgcactgg atcgtcgacg aacggcccga cgaggtgtcg gatcttctac
     2281 tggattggtt ggctgcgcac ccgactgcct aggtccagca ggccgcaccg cacatcgaac
     2341 cgctcgcgca cgacgttgag cgtgcgacgg taccgctcac gttcgcgacg gtgcgtgagc
     2401 atgaaccgcg ccgtagcaag cgtgcgccgg ggcaaaggaa gccagttgtt gaagtcgatc
     2461 ccgcattgcg agaacacctt attgggcgct gcctcgaaga taatgctcga gttgtgcagg
     2521 ccgagtgcga aaagcgtcag cgtgaacgcc gaattcgcct tctttcgcag ctccatcgca
     2581 tcgagctcat acagcgggac gtccctgatc ttgcccagca tcatcagatg cgtcatgaaa
     2641 taggccatga acgtggacaa ttggagcgtg acggaacgcg gattgatcga gagcaccgca
     2701 ccgctacgag agacataggg ttcatagggg cggtcgtcgt ggttgaggta tcccgtgcag
     2761 ttcactaccc agctgcccgg ctggatcgat tttgtggatc cgctgcgcaa caccaggtcg
     2821 gtgctgtcat tgcggtcgac gacgtcctcg agatggtcca tgaccacatc gttcaagccg
     2881 gcggcgatcg tcttgctctc cgagtccgac atcagtccga gcaggaagtt accggtctgc
     2941 ggcgtcaggt gaagaccata tgtatcgcgg aaccaggcgt tcacagccga ctcgttggtg
     3001 ccgtcgaaac gatgcgccac gtccatcgcg aggctgttga atgacttgcc gcgccaccac
     3061 cgtgcgagac cggtcggaag aacctggtcg cggcacagga agtacgtgcc cgaacccgcg
     3121 acgaggttca cctcgcggcc agggtacgag gtgatcaacg cgtgcgcggt atccatcgcg
     3181 gtctttccgc cgccgatgat ccagaccggg tcgtcgctcg cgccgatgtc gtctgtccgc
     3241 acatcgcaga agttgggtga caccgactga acgcgctcgc tcgacagctc gagtgggtcg
     3301 ttcggcttgc actgaaagcc ataggctttg atcaaccgct tcgtctcgat gaccaggcgc
     3361 cgaccgtcag aagatctgca ggtgacccgt actatcccgg cggtctcgtc ctgggactcg
     3421 acttcccagc cgaagagttc gtcgacccgc acgcgtgcct tgatgacgtt gaggcagtgc
     3481 gcgaagtggt cgagcacctc accctgggtc gccaggtatg agcgactccg gccaagtgtc
     3541 cacttgatgt cgcctgcggt gaacatggga tgcggctggt gcagtcgcac atacggatag
     3601 gtatcgaccc acatgccacc gacgcgtgcc cgccgatcca ccaggatgag cttctggtcc
     3661 cgcgacagat actggctggc gacgaacaac gcgttgagcc ctgcgacgcc tgcgcccacg
     3721 acacagacgt cgcagctctc cacggctacg ccgggagtcg catcggattg ttgaacggtc
//
LOCUS       DASYLX010000045         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2964335,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000045 DASYLX010000000
VERSION     DASYLX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964335"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGP27_01130"
     CDS             complement(<1..690)
                     /locus_tag="VGP27_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7849952.1"
                     /translation="MGVPTAYLRDRITRGVVVAASLFLVAGCTVSPPPAPQSTDTTET
                     TAPPPPKATQIIMAIDTIGPGFNSHLLSDQSPVNAAISSLVLPSSFRPVPDPASPTGS
                     RWEIDPTLLLSAEVTNDNPFTVTYKIRPEAQWTDNAPIAADDYWYLWRQMVSQPGVVD
                     PAGYDLITGVQSIEGGKTAVVTFSQPYPAWQELFNDILPAHIVKDVPGGFAAGLARAL
                     PVTGGQFRVENI"
     gene            1130..2266
                     /locus_tag="VGP27_01135"
     CDS             1130..2266
                     /locus_tag="VGP27_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849953.1"
                     /translation="MKERVQGAVTAGVALVGAGVMAAAPAAQEAPEVLRSVNADVALV
                     AAAVQPPYTQTVPQLFALSAQRTVTGLASTPVGLATAAIALAQGQNPAAQAILKEIVD
                     GPQWAADPAIYAFDDLLPEPLGGDDANNPRQPDANSAISQFRAAVLIAARNDVNEAIA
                     DSLGVGSAPQPAGNEVSPAYAGARLAGGLGVSAVRAAQSAVTAPLGLAAVAQGLQKSL
                     DGGGNKELYLAVQAYIDGPNYVTDPIVFAVDDVLPQAAGGGDPETNPAEMSGSDISRL
                     RGNVLLAPRDNVRKVVAGALGVDPIKGNPVPSATANKLVTNSDTAERKAPSSRVLTSL
                     RATSGGTATASTVGKHRAPATGGLSNLFKKKDDQKKADTTPAAE"
     gene            complement(2335..>2659)
                     /locus_tag="VGP27_01140"
     CDS             complement(2335..>2659)
                     /locus_tag="VGP27_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV7849954.1"
                     /translation="TQLSDRGTFFVDPGEDTYAGQVVGINPRPEDLDLNVTREKKLTN
                     MRSSTADVFETLAKPIELDLERAMEFCATDECVEVTPEFVRVRKVELDATTRARSRAR
                     AKARG"
BASE COUNT          506 a          839 c          845 g          469 t
ORIGIN      
        1 gatgttctcc actcgaaact gaccgcccgt aaccggcagt gctcgcgcca gcccggctgc
       61 gaacccgccc ggcacgtcct tgacgatgtg cgcgggcagg atgtcgttga acaattcctg
      121 ccacgccgga tacggctgcg aaaaggtcac gacggccgtc ttgccgccct cgatggattg
      181 gacaccggtg atgaggtcgt agccggcggg gtcgacgacg ccgggttggc tgaccatctg
      241 ccgccagagg taccagtagt cgtcagcggc gatcggcgcg ttgtccgtcc actgcgcttc
      301 gggccggatc ttgtaggtga cagtgaatgg attgtcgttc gtcacctccg ccgacagcag
      361 cagcgtcggg tcgatttccc accgcgagcc cgtcggcgac gccggatctg gaacgggccg
      421 gaatgagctc ggcaggacca acgagctgat cgccgcgttg accggcgact gatcggacag
      481 caggtgtgaa ttgaagcccg ggccgatggt gtcgatcgcc atgatgattt gcgtcgcctt
      541 cggtggcgga ggcgccgtgg tttctgtggt gtcggtgctc tgcggcgcag gtggcggact
      601 caccgtgcac ccggcgacga ggaataacga tgctgcgacg actacccctc gcgtgatccg
      661 gtcacgaagg taggcggtcg gcacgcccaa cagggtaacg acagccgtcc cgaggtccga
      721 ttcgcgagcc gacaagttag cgtagttaga taatttacga gaccgtcaca gcaaacatag
      781 tttgctggct gtgctcggac acgttcagta gcttcatgca tcggcaggta ggcatcggcg
      841 acgccgggcg cactgggctg ccagtccgcc gatgccccga atctgaggac gcctcaagag
      901 gctatacagc cacttccact gattatcgca atttgcattc aaccctgaga gtttcttatt
      961 agactcccta cccctgcgca gcggcggcgg ttttcgacgg catcgacacg aacacaccgc
     1021 tcgacgtgcc gcaacggcca agttgctcag ctaacttgtt ttgaaccgat tcgagcccta
     1081 agcttcggcg cgagatcggg ttgatccaaa accaaaaccg agggggaaca tgaaagaacg
     1141 tgtgcaaggc gctgtcacgg cgggtgtggc gcttgtcggt gcgggggtga tggccgcagc
     1201 gccggccgcg caggaagcgc cggaggttct gcgaagcgtc aacgccgacg ttgcgctcgt
     1261 ggcggccgct gtccaaccgc catacacaca gacggtgccc cagctcttcg ctctgtccgc
     1321 acaacggacg gtaactggac tcgcgtcgac gccagtcggc ctcgcaacgg ctgcaatcgc
     1381 gctcgcacaa ggccagaacc cggccgctca ggcgatcctg aaagagattg ttgacgggcc
     1441 gcagtgggcc gctgatccgg cgatctatgc gttcgacgat ctccttccgg agccgctcgg
     1501 tggcgatgac gcaaacaatc ccaggcagcc ggacgcaaat agcgctatct cgcaattccg
     1561 tgccgctgtg ctcatcgctg caagaaatga cgtcaacgaa gcaatcgccg acagcctggg
     1621 cgttgggtcg gcgccccagc cggccggcaa tgaggtcagt cctgcctatg ccggggccag
     1681 gcttgcgggt ggtctcggcg tatctgctgt gcgcgccgcc cagagcgctg tcaccgcgcc
     1741 gttggggcta gcggccgtcg cgcagggcct ccagaagagc ctcgatggcg gcggcaacaa
     1801 ggagctgtac ctggcagtcc aggcatacat agatgggccg aactacgtca cagaccccat
     1861 cgtgttcgct gtcgacgatg tgctgccgca ggccgcaggc ggtggcgatc cggagacgaa
     1921 tcccgccgag atgagtggca gcgatatttc acgtctccgc ggcaatgtcc ttctcgcgcc
     1981 tagggataac gtccgcaagg ttgttgccgg cgctctcggc gtcgacccga taaagggcaa
     2041 ccctgtgccg agcgcaacgg ccaacaagct ggttacgaat agtgacaccg cagagcggaa
     2101 agccccaagc agtcgagtcc tcacctcgct gagggccacc tccggtggga ctgcgaccgc
     2161 cagcacggtc ggcaagcatc gcgctcccgc aaccggtggc ctgagcaatc tcttcaagaa
     2221 gaaggacgac cagaagaagg cggacaccac cccggcggcc gagtaactga acgatcgaaa
     2281 ggccggccgc ggcgcgatcc gtggcaggct ttttcgtcgg ctaaagggcc ttaactaacc
     2341 gcgcgctttc gcccgggccc gactacgggc cctggtggtt gcgtccagtt cgaccttgcg
     2401 cacccgcaca aactcgggcg taacctcgac gcactcgtcg gtcgcacaaa actccatggc
     2461 gcgctccaga tcgagctcga tcggtttggc aagtgtctca aagacatccg ccgtcgacga
     2521 ccgcatgttg gtcagcttct tctctcgcgt gacgttgagg tcgagatcct ccggtcgcgg
     2581 attgatcccg acaacctggc cagcgtaggt gtcctccccc ggatcgacga agaacgtgcc
     2641 gcggtcggac agttgagtc
//
LOCUS       DASYLX010000046         7019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1977400,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000046 DASYLX010000000
VERSION     DASYLX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977400"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="cysT"
                     /locus_tag="VGP27_01145"
     CDS             <1..690
                     /gene="cysT"
                     /locus_tag="VGP27_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEV7849955.1"
                     /translation="GWSAFWSTVTSKAAVESFRVTLTVSIGVSLINLVFGLLVAWVLT
                     RDDFPGKRLVDSVIDLPFALPTIVASLVMLALYGPNSPVDLHLQHTKWGIGVALLFVT
                     LPFVVRSVQPVLLELDRETEEAAASLGANNWTIFTKVVLPALLPSLLSGAGLAFSRAI
                     GEFGSVVLIGGAVPGETEVSSQWIRTLIENDDRTGAAAISIVLLVISFVVLFILRAIG
                     SRAARREELAR"
     gene            687..1502
                     /gene="cysW"
                     /locus_tag="VGP27_01150"
     CDS             687..1502
                     /gene="cysW"
                     /locus_tag="VGP27_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEV7849956.1"
                     /translation="MTLSPVVRHLLRYLALAYVVILVIVPVALILWRTFQPGIGAFFE
                     SITTPAALSALQLSLLVVAIVVPLNVIFGVPTALVLARNRFRGKSALQAVIDLPFAVS
                     PVVVGVALILLWGSAGLFGFVENNLGFKIIFGFPGIVLASIFVTVPFVIREVEPVLHE
                     IGTDQEEAAATLGSQWWQTFGRVTLPSIRWGLTYGIVLTIARTLGEFGAVIMVSSNLP
                     GTSQTLTLLVADRYTRGAEYGAYAVSTLLMTVAVLVLIVQVVLDARRARQIKA"
     gene            1514..2563
                     /locus_tag="VGP27_01155"
     CDS             1514..2563
                     /locus_tag="VGP27_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEV7849957.1"
                     /translation="MTDAIIVRGANKHYGDFAALDNVDFVVPDGSLTALLGPSGSGKS
                     TLLRAIAGLDQPDTGTVTIKGKDVTNVPPQRRGIGFVFQHYAAFKHLTVRDNVAFGLK
                     IRKRPKPEVKEKVDNLLEVVGLAGFQTRYPNQLSGGQRQRMALARALAVDPQVLLLDE
                     PFGALDAKVRDDLRAWLRRLHDEVHVTTVLVTHDQAEALDVADRIAVLNKGRIEQVGS
                     PTEVYDTPANAFVMSFLGAVSSLNGTLVRPHDIRVGRNPDMAIANTDDSVQSTGVLKA
                     TIDRIVTLGFEIRVELTAASNNVGFTAQITRGDAEALGLKEGDIVYVRATRVPPLPGG
                     ADLPKVDDNEALTNA"
     gene            complement(2591..3826)
                     /locus_tag="VGP27_01160"
     CDS             complement(2591..3826)
                     /locus_tag="VGP27_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7849958.1"
                     /translation="MTVTRDAVAPRKSFSGRPLDGSVEDRLARLPEVVAALRRDDAAA
                     ERGRILQYEAVESIWRTGVLNLRVPTEFGGPGGKARDVLSAVIQIGRGSSNVAQALRS
                     HFGFSERLLSNRATRQEAKEWFPRVYAGLIVGNAITDAAGKAPSSADTKVLPDTDGVL
                     RLNGYKFYSTGTLFADVIAVSALDADGRDVQAIIPADRPGVDLFDDWDGFGQRTTASG
                     GTAFTDVEVHPREVVTVSDGNSLGHATGFLQLYLAAVAVGIAYAVFDDAVEYVRTKAR
                     PASHSVADSAATDPFVLEAVGEISAAATSAEAIVLTAADAIDRLVDEGTQSDPDVVAD
                     VAVTVAGAQLVAERLTISAAQRLFDTGGASATARALNLDRHWRNARTIAGHSPLAYKA
                     YAAGNYAVNGTWPPANGYF"
     gene            complement(3841..4857)
                     /locus_tag="VGP27_01165"
     CDS             complement(3841..4857)
                     /locus_tag="VGP27_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7849959.1"
                     /translation="MTAPRFGVWAPVYGNHGARSHPRDAPDAGYRRTRDLLLRAERAG
                     FDSTLLAEHVIHPSNTEDDVLETWSTIAALAEATSSIELIGAVKPLLFNPLVFAKIAA
                     NIADIADGRVAVNLVTGWFLPELEGLGLDPLDHDDRYAYSMKWIRIVTELWAGKHVAI
                     GDRDGQPALVRPLPPGPPLLYVGGESEPGRALAAETADVFFINGRPFADTVAVIEDLR
                     ARPRDGAPLRFGLSAFVIARETEAEAAAELSYLQSLSDAESRPEISGGTDPDTQMYKV
                     LSGTKRIGSNGGTLAGLVGSYDKVIERIEAFHDAGVELFMLQFQPIEAEVDRFADRII
                     PHFR"
     gene            complement(4854..6209)
                     /locus_tag="VGP27_01170"
     CDS             complement(4854..6209)
                     /locus_tag="VGP27_01170"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV7849960.1"
                     /translation="MTHTVNSTSTGNRVRSAPEQELRNGLTDGSLAEIEIAWSDPFGH
                     AQGKRIPASQFSSRALGTGFAFCEASLGWDTAGTVIDSLRLTNWSGGYPDVYAVPDFS
                     TYRPLPWRPRVGHVISDIVAHDRRPSLLDPRAVLKKVLARLSALGFTAKVGVEFELYL
                     LNTDGSAFQDDIHAYSLENANALDPILADLYETLSAFTALEGIQTEYGPGQIETNLVY
                     TDALEAADDAARLKYAAKEVARRHGKIASFMPKPFTEHSGSSQHLHISLWRGGEPAFA
                     PDGGAENALALHAIAGLLEHLPSITLFGAHSVNAYRRFTPDSFAPATVTWSRDNRSAA
                     VRSLIESEPAATRIELRSGASDANPYWLIASALAAVVAGLEAERTPPAALGGNLYTEG
                     VRLPESLGVAIELATRDDTILEILGADSVLDFASLARSEWAEFSTEVSDWERRRYLSR
                     S"
     gene            complement(6206..>7019)
                     /locus_tag="VGP27_01175"
     CDS             complement(6206..>7019)
                     /locus_tag="VGP27_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7849961.1"
                     /translation="SGSLGIGLLFALISYVGFEATAIFRDEARTPDRTIPRATYVALI
                     LIGVFYAVTSWALISGWGDDEALRRATDSGSTFLADTAQRYIGVVGTDIITVLYFTSL
                     FACILSFHNVASRYVFALSQRDVLPESLSRPHDRHGSPHQASLWISGVVAVSIIAAAV
                     FDLDPAAQFYTWFAGATTVGFVVLLIATSVAVLVFFGKDRRGYSQWRVRIAPALGLVG
                     LAAGLVLIVINLEGLVGGSTVLGWSIVGLLVVAFAAGAVVGTRVGDKAVSRQ"
BASE COUNT         1163 a         2239 c         2383 g         1234 t
ORIGIN      
        1 ggctggagtg ccttctggtc gacggtcaca tcgaaggcgg ccgtcgagtc gttccgggtg
       61 acactcacgg tctcgatcgg cgtgagccta atcaatctcg tcttcggatt gctggtggcg
      121 tgggtactca cccgcgacga cttccccggt aagcgcctcg tcgactcggt gatcgacctg
      181 ccgttcgcac taccgacgat cgtcgcgagc ctggtcatgc tggcgctcta cggcccgaac
      241 agtccggtgg accttcacct gcagcacacc aagtggggca tcggggtggc gctgttgttc
      301 gtaacgctgc cgttcgtggt gcgctcggtg cagccggtgt tgttggagct cgaccgggag
      361 accgaggagg ccgccgcctc gctcggcgcc aacaactgga cgatcttcac caaggtcgtc
      421 cttcccgcgc tgctgccgtc attgctgtcc ggagcgggac tggccttctc cagagcgatc
      481 ggcgagttcg gctctgtcgt gttgatcggc ggggcggtgc cgggagagac ggaagtgtcg
      541 tcgcagtgga tccggacgct gatcgagaat gacgaccgaa ccggcgccgc tgctatttcc
      601 attgtgctgc tggtgatctc attcgtggtg ctgttcatcc tgcgtgccat cgggtcgcgg
      661 gcggccagac gtgaggagtt ggcgcgatga cactgtcacc ggttgtgcgc catctgctgc
      721 gctaccttgc gctcgcctac gtcgtcatcc tggtgatcgt gccggtggcg ttgattctgt
      781 ggcgtacctt ccagcccgga atcggggcct tcttcgaatc gatcaccacg ccggcggcgc
      841 tgtcggcact gcaattgtcg ctgctcgtcg tggcgatcgt ggtcccgctg aacgtgatct
      901 tcggtgtacc gaccgctcta gtattggcgc gcaacaggtt tcgcggcaag agcgcactgc
      961 aggcggttat cgacctgccg ttcgccgttt cgccggtcgt ggttggcgtc gcgttgatcc
     1021 tgctctgggg ctcggccggg ctgttcgggt tcgtcgagaa caacctcggc ttcaaaatca
     1081 tcttcggttt tccgggcatc gtgctggcga gcatcttcgt gacggtgccg ttcgtgatcc
     1141 gagaagtcga acccgtgctg cacgagatcg gcaccgacca ggaggaggcg gccgccacgc
     1201 tgggatcgca gtggtggcag accttcgggc gcgtgacgtt gccgtcgatc cggtggggcc
     1261 tgacctatgg catcgtcctg acgatcgcgc gcacgctcgg cgagttcggt gcggtcatca
     1321 tggtgtcgtc caacctgccg ggaacctcgc agactctcac actgctggtc gcggaccggt
     1381 acacccgcgg cgcggagtac ggcgcatatg ccgtgtccac gctgctgatg actgtggctg
     1441 tgctcgtcct gatcgttcag gtcgttttgg acgcccgacg tgcgcggcag atcaaggcat
     1501 aggagaactg gcaatgaccg acgcaatcat cgtgcgaggt gcgaacaagc actatggcga
     1561 cttcgccgcg ctggacaacg tcgacttcgt ggtgccggac ggctcgctga cggccctgtt
     1621 gggtcccagc ggctcgggta agtccacgct gttgcgcgcg atcgcgggcc tggatcagcc
     1681 cgataccgga accgtcacga tcaagggcaa ggatgtgacg aacgtgccgc cgcagcggcg
     1741 cggtatcggc ttcgtctttc agcactatgc ggccttcaag catttgaccg tgcgcgacaa
     1801 cgtcgcgttc ggtctcaaga tccgcaagcg cccgaagccg gaagtcaaag aaaaagtcga
     1861 caacctactg gaagtggtgg gactcgccgg ttttcagacc cgttatccca accagctgtc
     1921 gggcggtcag cgccaacgca tggcgttggc gcgcgcgctc gcggtcgatc cgcaggtgtt
     1981 gctgctcgat gagccgttcg gtgcgctcga cgcgaaggtg cgtgacgatc tgcgcgcgtg
     2041 gctgcgccgt ctgcacgacg aggtgcacgt cacgacggtg ctcgtcacgc acgaccaggc
     2101 ggaggcgctc gacgtcgccg accggatcgc cgtcctcaac aagggccgta tcgagcaggt
     2161 cggctcgccg acagaggtgt acgacacccc ggccaacgcc ttcgtgatgt ccttccttgg
     2221 ggcggtgtcc tcgctcaacg gcacgttggt gcggccacac gacatccgcg tgggtcgcaa
     2281 tcccgacatg gcgatcgcca acaccgacga ttcggtgcag agcaccggcg tgctcaaggc
     2341 cacgatcgat cgaatcgtca cactgggctt cgagattcgc gtcgagctga ccgcggcatc
     2401 gaataacgtc ggtttcaccg cgcagatcac ccgcggtgac gccgaggcgc tcggactgaa
     2461 agagggcgac atcgtctatg tccgcgccac ccgggtgcct ccgctgccgg gcggcgccga
     2521 cctgcccaag gtcgacgaca acgaggcgct gaccaacgcc tgaccttgct ccgcgagact
     2581 ggaagcgggg ctagaagtag ccgttggcgg gcggccacgt gccgttgacg gcgtaattgc
     2641 ctgccgcgta cgccttgtac gccagcgggc tgtgcccggc gatcgtgcgc gcattccgcc
     2701 agtgccggtc caggttcagc gcccgggcgg tcgccgatgc gccgccggtg tcgaagagtc
     2761 gctgggcggc cgagatcgtc agccgctccg cgaccaactg tgccccggcc acagtgacgg
     2821 cgacgtcggc gaccacgtcg gggtcgctct gcgtgccctc gtcgacaagg cggtcgatgg
     2881 cgtcggcggc cgtcaacacg atggcttcgg ccgacgtcgc cgccgccgag atctcgccga
     2941 cggcttccaa cacgaacggg tcggtggctg cgctgtccgc caccgaatgt gacgccgggc
     3001 gcgccttcgt gcgcacgtat tcgacggcgt cgtcgaacac cgcgtatgcg atgcctactg
     3061 ccacggccgc cagatacagc tgcaggaaac ccgtcgcgtg ccccaggctg ttgccgtcgg
     3121 acacggtgac gacctcgcgg ggatgcacct cgacgtcggt gaatgccgtt ccgccgctgg
     3181 cggtggtgcg ctgaccgaat ccgtcccagt cgtcgaacaa gtcaactccg ggacggtccg
     3241 ccgggatgat cgcctgcacg tcgcgaccgt ctgcgtccag cgccgacacc gcgatgacgt
     3301 cggcgaaaag cgtgccggtc gaatagaact tgtaaccgtt gagccgcaaa accccgtcgg
     3361 tgtcgggcag cactttggtg tcggcactcg acggggcctt gcccgccgca tccgtgatgg
     3421 cgtttccgac gatgaggccc gcgtagacgc gcggaaacca ttccttcgcc tcttgccgtg
     3481 tggccctgtt gctgagcagc cgctcggaga agccgaagtg ggaccgaagg gcctgtgcca
     3541 cattcgaact gccgcgaccg atctggatca cagccgacag cacatcacgc gccttcccgc
     3601 cggggccgcc gaactcggtc ggcacccgga ggttgagcac accggtccgc cagatcgact
     3661 cgacggcctc gtactgcagt atccgcccgc gctcggcggc tgcgtcgtca cgacggagtg
     3721 cggcgacgac ctcgggtagc cgcgcgagtc ggtcttcgac cgacccgtca agcggtcgac
     3781 cggagaacga ctttcggggt gcgacggcgt cgcgtgtgac agtcatgtcg gctcccagcg
     3841 tcaacggaaa tggggaatga ttctgtcggc gaaccggtcc acctcggcct cgatgggctg
     3901 gaactggagc atgaacagct ctactccggc gtcatggaat gcctcgatgc gctcgatcac
     3961 cttgtcgtag ctgccgacca gaccggcgag ggtaccgccg ttggaaccga ttcgtttggt
     4021 gcccgacagc actttgtaca tctgagtgtc cggatcggtg ccaccggaga tctcgggccg
     4081 gctctcggca tccgacagcg actgcaggta gctgagttcg gcggcggcct ccgcctcggt
     4141 ctcgcgggcg atcacgaagg cggacaggcc gaatcgcagc ggtgcaccgt ctcgaggtct
     4201 cgcacgcagg tcctcgatga ccgcgaccgt gtcggcgaac gggcggccgt tgatgaagaa
     4261 gacatcggcg gtttcggcgg caagggcgcg gccgggctcg gattcgccgc cgacgtacaa
     4321 cagcggcggg ccgggcggta gcggccggac cagcgcgggc tggccgtcgc ggtcgccgat
     4381 tgcgacgtgc ttgccggccc acaactcggt gacgatcctg atccatttca tggagtaggc
     4441 gtaccggtca tcgtgatcga gcgggtccag gcccaacccc tcaagttcgg gcaggaacca
     4501 cccggtgacg aggttgaccg cgactcgtcc gtcggcgatg tcggcgatgt tggcggcgat
     4561 cttcgcgaac accaacgggt tgaacagcag tggtttgacc gcaccgatca actcgatgga
     4621 tgaggtggcc tccgccaacg ccgctatggt ggaccatgtt tcgagtacgt cgtcttcggt
     4681 gttgctggga tgaatcacgt gttctgccaa caacgtcgag tcgaagccgg cccgctcggc
     4741 gcgcaacagc aggtcgcgcg tccgccggta gccggcgtcc ggcgcgtcgc gcggatgaga
     4801 ccgggcgccg tgattgccgt ataccggcgc ccatacgccg aaccggggag cggtcacgag
     4861 cggctcaggt agcgccggcg ttcccagtcg ctgacttcgg tagagaactc cgcccactcg
     4921 ctgcgggcca acgaggcgaa gtcgagaacc gagtcggctc ccagtatctc caggatggtg
     4981 tcgtcacgag tggccagttc gattgccaca cccagtgatt cgggcaaccg aaccccttcg
     5041 gtgtagaggt tgccgccaag ggcggccggc ggcgtgcgtt cggcttccag ccccgcgacg
     5101 accgctgcca gggccgatgc gatcagccag taggggttcg cgtcggaggc accgctgcgc
     5161 aattcgatcc gggtggcggc gggctcggat tcgatcagcg atcgcacagc ggcgctgcgg
     5221 ttgtcccggc tccaggtcac cgtcgcaggc gcgaacgagt ccggggtgaa ccgccggtag
     5281 gcgttgaccg aatgcgcgcc gaacagcgtg atcgacggga gatgctcgag cagtcccgcg
     5341 atggcgtgca gggccaatgc gttttcggcg ccgccgtcgg gcgcgaaggc cggctcgcca
     5401 ccacgccaca gcgagatgtg caggtgctgc gagcttcccg aatgttcggt gaagggcttc
     5461 ggcatgaagc tggcgatctt gccgtgtctg cgggcgacct ctttggcggc gtatttgagc
     5521 cgggcggcgt cgtccgccgc ttccagcgcg tcggtgtaga ccaggttcgt ctcgatctga
     5581 ccgggaccgt attcggtctg gatgccctcc aatgccgtga acgcgctcaa cgtttcgtac
     5641 aggtcggcga gtatcgggtc gagggcgttg gcgttctcga gcgaataagc gtggatgtcg
     5701 tcctgaaacg cagagccgtc cgtgttgagg aggtagagct cgaactcgac gccgaccttg
     5761 gcggtgaagc ccagcgcgga aaggcgcgcc agcaccttct tcagtacggc gcggggatca
     5821 agaagtgacg gacggcggtc gtgcgcgacg atatcggaga tcacgtggcc gacccgggga
     5881 cgccacggca acggccggta ggtcgagaag tctggaacgg cgtacacgtc ggggtagccg
     5941 ccgctccagt tggtcaaccg cagcgagtcg atcaccgtgc ctgcggtgtc ccagcccaga
     6001 gatgcttcgc agaaggcgaa cccggtgccg agcgcccggc tcgagaactg tgatgccggg
     6061 attcgtttgc cctgcgcgtg accgaacgga tcactccacg cgatctcgat ttcggccagc
     6121 gacccgtctg tcagcccgtt ccgcagttct tgctcgggag ctgatcgaac ccggttgccg
     6181 gtcgacgtcg aattgacggt gtgtgtcact gccgtgatac cgctttgtcg ccgactcggg
     6241 tgccgacgac cgcacccgcc gcgaacgcga cgacgagcag accgacgatc gaccagccca
     6301 gcacggtgga tccaccgacg aggccttcga ggttgatcac gatgagcacc agccctgccg
     6361 ccagcccaac cagtccgagt gcgggcgcga tacgcacccg ccactgcgag tagccgcgac
     6421 ggtccttgcc gaagaacacc agcacggcca ccgatgtggc gatcagcagg acgacgaacc
     6481 cgaccgtggt cgcgccggcg aaccaggtgt agaactgggc cgcagggtcg aggtcgaaca
     6541 ccgcggccgc gatgatgctg actgcgacca cacccgaaat ccacagcgag gcctgatgtg
     6601 gtgagccgtg ccggtcgtgc gggcgactca acgactcggg cagcacgtcg cgctgcgaga
     6661 gcgcgaacac atatcgcgac gccacgttgt ggaatgacag gatgcacgcg aacaggctgg
     6721 tgaagtagag caccgtgatg atgtcggtgc cgacgacacc gatgtagcgc tgcgcggtgt
     6781 cggcgaggaa ggtggagccg gaatccgttg cccgacgcaa cgcttcgtcg tcgccccagc
     6841 ccgagatcag tgcccagctg gtcaccgcgt agaagacgcc gatcaggatc agcgcgacgt
     6901 aggtggcgcg cggaatggtg cggtccggcg tgcgggcctc gtctcggaag atcgcggtgg
     6961 cctcgaaacc gacgtagctg atcaaagcga acagcagccc gatacccagc gagccggag
//
LOCUS       DASYLX010000047         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2416009,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000047 DASYLX010000000
VERSION     DASYLX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416009"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGP27_01180"
     CDS             complement(<1..443)
                     /locus_tag="VGP27_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7849962.1"
                     /translation="MNPPPALRPTLAGDYYFSDAVFASEQERIFENLWFCAVRSSEIA
                     SPGKFRKVQVGRESVLLIRGRDGLLRAFLNVCRHRGAQLCTEAEGEVKRSLRCSYHSW
                     TYALDGKLMAAPNIASLTDETGAAIDRYRYGLVPVALTEWLGYAWV"
     gene            complement(479..1612)
                     /gene="solA"
                     /locus_tag="VGP27_01185"
     CDS             complement(479..1612)
                     /gene="solA"
                     /locus_tag="VGP27_01185"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEV7849963.1"
                     /translation="MGYDVIVIGLGGMGSAAAYHLAARGQRVLGLEKFTPAHDKGSSH
                     GGSRIIRQSYFEDPAYVPLLLRAYELWDQLATDTGLEVYRLTGGLFMGPSNCLTVAGS
                     QRASQEWDLPHEMLDAAEIRRRYPTFTPYPGDIALYEAKAGFARPEMTVQAHITLAEK
                     AGAELHFGEPVIEWSEIAGGVRVTTAHGTYTAGQLVVCPGAWAPQLLAEFGIPITIER
                     QVLYWFDPRGGTAPFEDHPIFIHEDTTGAQAYGFPAIDGPDGGVKVAFFRKGIVCTPE
                     TIDRTVHDQEIAEMRDRVARVAPALDGPCVHSATCMYSNTPDEHFVIARHPDSENITV
                     ACGFSGHGFKFVPVVGEILADLAIDGVTGHPISLFDPKRLVTT"
     gene            complement(1621..>1965)
                     /locus_tag="VGP27_01190"
     CDS             complement(1621..>1965)
                     /locus_tag="VGP27_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEV7849964.1"
                     /translation="AGVDFAVKMTKDDFVGKAALERAAAPQKVLRSIVFDEPTAVVLG
                     KEPVYIAERCVGYVTSAAYSPTVGRTIAYAWLPAAAEVGDGVTVDYRGTRLAASVHAE
                     PVVDPEMARIKR"
BASE COUNT          319 a          644 c          669 g          333 t
ORIGIN      
        1 acccaggcgt agccgagcca ctcggtcagc gcgacgggaa caaggccgta tcggtagcgg
       61 tcgatcgccg ccccggtctc gtcggtgagc gacgcgatgt tgggcgcggc catcagcttg
      121 ccgtccaggg catacgtcca ggagtggtag gagcagcgca ggctgcgctt cacctccccc
      181 tcggcctcgg tgcacagctg agcgccgcgg tggcggcaga cgttgaggaa cgcccgcaac
      241 aaaccgtcgc ggccacggat cagcagcacg ctttctcgtc ccacctggac cttcctgaat
      301 tttcccggtg aggcgatttc ggatgaccgc accgcgcaga accacaggtt ctcgaaaatg
      361 cgttcctgct cggacgcgaa gaccgcgtcg ctgaagtagt aatcgcctgc gagcgtcgga
      421 cgcagggcgg gtggtgggtt catcgcgggc ggcccgagaa ggggcgggaa atctggaatc
      481 atgtcgtcac cagtcgcttc gggtcgaaca acgagatggg gtggccggtc acgccgtcga
      541 tggcgaggtc ggcgaggatt tccccgacga cggggacgaa tttgaacccg tggcccgaga
      601 agccgcatgc cacggtgatg ttctcggaat ccgggtggcg agcgatgacg aaatgctcgt
      661 cgggcgtgtt ggagtacatg cacgtcgccg agtgcacgca gggcccgtcg agcgcgggcg
      721 ccacccgggc gacgcggtct cgcatctcgg cgatctcctg gtcgtgcacg gtccggtcga
      781 tggtctccgg tgtgcacacg atgcccttgc ggaagaacgc caccttcaca ccgccgtcgg
      841 gtccgtcgat cgccggaaag ccgtatgcct gcgcgccggt ggtgtcttcg tggatgaaga
      901 tcggatggtc ttcgaaaggc gctgtgccgc cacgcgggtc gaaccagtac agcacctgac
      961 gttcgatggt gatcggaatg ccgaattcgg cgagcagctg cggcgcccat gcgccggggc
     1021 agacgacgag ctgtcctgcg gtataggtgc cgtgcgccgt cgtgaccctg acaccaccgg
     1081 caatctcgct ccactcgatg accggttcgc cgaaatgcag ctcggcaccg gctttttcgg
     1141 cgagggtgat gtgcgcctgg acggtcatct ccgggcgggc gaaccccgcc ttcgcttcgt
     1201 agagggcgat gtcgccggga tacggcgtga atgtcggata gcgacgccga atctccgcgg
     1261 cgtcgagcat ctcgtgcggc aggtcccatt cctggctggc gcgttggctt ccggcgacgg
     1321 tcaggcagtt cgacggcccc atgaacaggc cgccggtcag ccggtagacc tcgagcccgg
     1381 tgtcggtggc gagttggtcc cacagctcgt aagcccgcag gagcaacggc acgtacgccg
     1441 ggtcctcgaa gtaggactgc cgaatgatgc gcgacccgcc gtggctggag cccttgtcgt
     1501 gtgcgggagt gaacttctcc agccccagca cccgctgacc ccgcgcggcc aggtggtagg
     1561 cggcggcgct gcccatcccg ccgagaccga tgacgatgac gtcgtaaccc attgtttgcg
     1621 ttacctcttg atccgcgcca tctcgggatc gacgaccggc tctgcgtgca cgctggccgc
     1681 caaccgcgtg ccgcgatagt cgacggtgac gccgtcaccg acctcggccg cggcaggcag
     1741 ccacgcgtag gcgatggtgc gcccgaccgt cggcgaatac gccgcgctgg tcacatatcc
     1801 gacgcagcgc tcggcgatgt acaccggctc cttgccgagc acgacggcgg tcggttcgtc
     1861 gaacacgatg ctgcgcaaca ccttctgcgg tgccgccgcg cgctccagcg ccgccttgcc
     1921 gacgaagtcg tccttggtca tcttgaccgc gaagtccacg cccgc
//
LOCUS       DASYLX010000048         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_660805,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000048 DASYLX010000000
VERSION     DASYLX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660805"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGP27_01195"
     CDS             <1..349
                     /locus_tag="VGP27_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7849965.1"
                     /translation="QEWMCPRPVRVVDVTVEPHVGGTVRFDVDDSGTRVLITGQFLTI
                     DRPRLLRFTWTNSNWPDPTRESIVNVAFEPVGEDETLMSIEHSLLPPEEFESFESGWT
                     LTSEQLSCALEQR"
     gene            346..729
                     /locus_tag="VGP27_01200"
     CDS             346..729
                     /locus_tag="VGP27_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV7849966.1"
                     /translation="MTQRWIVDAMNVIGSRPDGWWKDRRQAMVKLVDQLERWAPGQDH
                     RVTVVFEKPMSPPIDSSVIEIANAPRAAANSADDEIVRLVEADDQPQDLTVVTSDSAL
                     SERVRNAGASIFPAARFRDLIEPVR"
     gene            complement(754..1056)
                     /locus_tag="VGP27_01205"
     CDS             complement(754..1056)
                     /locus_tag="VGP27_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849967.1"
                     /translation="MVTVKRGVVVFGWAVALAAAPLVCASPAAAKECPWGTKLTHWNT
                     CVSGAGTAAAPVVPVPPSGGGGPNIVNNPNGYDTVNGIPCSAEHYGTCYGMIQNGG"
     gene            1139..1385
                     /locus_tag="VGP27_01210"
                     /pseudo
     CDS             1139..1385
                     /locus_tag="VGP27_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099777.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
     gene            1465..>1656
                     /locus_tag="VGP27_01215"
     CDS             1465..>1656
                     /locus_tag="VGP27_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7849968.1"
                     /translation="MTIGDDEVSRAVQADSGTQQVQVRRAAMASAIGTTIEWYDFFLY
                     NTAAALVFPHLFFPESTAYA"
BASE COUNT          291 a          542 c          525 g          298 t
ORIGIN      
        1 gcaggagtgg atgtgtccgc gtcctgtccg cgtggtcgat gtcaccgtcg aaccgcatgt
       61 cggcggtacc gtgcgcttcg atgtcgacga ctcgggcact cgcgtcctga tcaccggcca
      121 gttccttacc atcgaccgac cacgactgct gcgcttcacc tggaccaact cgaattggcc
      181 ggacccgact agggaaagca tcgtcaacgt cgccttcgag ccggtcggcg aggacgagac
      241 actgatgtcg atcgagcatt ccttgcttcc cccagaggaa ttcgagagct tcgagagcgg
      301 ctggacgctc acctccgagc agctctcctg tgcgctggag cagagatgac gcagcgctgg
      361 atcgtcgacg cgatgaatgt gatcggcagc cgacccgacg gctggtggaa ggatcgccga
      421 caggcgatgg tcaagcttgt cgaccagctc gagcgctggg cgccaggcca ggaccaccga
      481 gttaccgtgg tgttcgagaa gccgatgtca ccgccgatcg actcatcggt gatagagata
      541 gccaacgcgc cccgagcggc cgccaactcc gcggacgacg agatcgtcag gctggtcgaa
      601 gcagacgatc aacctcagga tctcaccgtc gtgacgtcgg acagtgcgct gtcggagcgg
      661 gtccgaaacg cgggcgcttc gatctttccg gctgcgaggt ttcgcgacct catcgagccg
      721 gtgcggtaat cggggcgtcc ggcgacgagg gagtcagccc ccgttttgga tcatgccgta
      781 gcacgtgccg tagtgctctg cgctgcacgg aatgccgttg accgtgtcgt atccgttggg
      841 gttgttgacg atgttcggac cgccgccgcc ggacggcgga acggggacta ccggcgctgc
      901 tgcggtgccg gcgccactga cacaggtgtt ccagtgggtc agcttcgtgc cccacggaca
      961 ttccttggct gcggcgggcg atgcgcacac caatggtgct gcggccaagg ccaccgccca
     1021 tccgaaaacc accacaccgc gcttgacagt caccatctaa gctctccgaa cctctcgccc
     1081 gacccgcaac agcctaaccc gagagcttct gacttgcggc cgtgtccctc ttttcccggt
     1141 gtgggcagcg ttgcgcgcgg ccgtcgcaaa ccttcctgac gaggccttcg acgagccgtc
     1201 cggctgtgcg cattggctgg ttcgcgaccc ggtgtgtcac ctggtcgtcg acgcgcagga
     1261 cgtgctgatc accctggtga caccgctcaa agcgagccga ccagggaccg gtgacctacc
     1321 gggaggtggc tgaaacgccg ccgttgggcg acgacccact cggcgcgctg accgtccggc
     1381 cggcggcggg cttccccatc cggccgtggc ggtacagact ggcaactgac tgtcaacgaa
     1441 aatcggttac agggaggcaa tccgatgact atcggggacg acgaggtcag ccgcgcagtt
     1501 caagccgatt ctggcaccca acaggttcag gttcggcgtg ctgcgatggc aagcgcgatc
     1561 ggcacgacga tcgagtggta cgactttttc ctctacaaca cggccgccgc acttgtcttt
     1621 ccgcacctct tcttccccga atcgacggcc tacgcc
//
LOCUS       DASYLX010000049         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2854959,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000049 DASYLX010000000
VERSION     DASYLX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854959"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="raiA"
                     /locus_tag="VGP27_01220"
     CDS             <1..585
                     /gene="raiA"
                     /locus_tag="VGP27_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV7849969.1"
                     /translation="KGRNVEIPDHFRIYVSEKLARLERLDRSIYLFDVELDHERNRRQ
                     RKNCQHVEITARGRGPVVRGEACADSFYAALESAVCKLENRLRRSKDRRKVHYGDKTP
                     VSLAEATKVAAPEAFDPTGADDGAPHDGVEIHEPGQIVRTKEHPAKPMTVDDALYEME
                     LVGHDFFLFHDKQSDRPCVVYRRHAYDYGLIRLA"
     gene            757..>2210
                     /gene="secA"
                     /locus_tag="VGP27_01225"
     CDS             757..>2210
                     /gene="secA"
                     /locus_tag="VGP27_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV7849970.1"
                     /translation="MLDKLLRFGEGRMVKRLKRVSDYVNTLSDDVEKLSDTELRAKTD
                     EFKKRYADGEDLDDLMPEAFAVAREAAWRVLDQRPYDVQVMGGAALHFGNVAEMKTGE
                     GKTLTCVLPAYLNALSGKGVHVVTVNDYLAKRDSEWMGRVHRLLGLEVGVILAQMTPD
                     QRRVAYNADITYGTNNEFGFDYLRDNMAHSSEEMVQRGHAFGIVDEVDSILIDEARTP
                     LIISGPADGSSNWYSEFARLAPLMEKDKHYEVDIRKRTIGVSELGVEFVEDQIGIDNL
                     YEAANSPLVSYLNNAIKAKELFMRDKDYIVRDGEVLIVDEFTGRVLLGRRYNEGMHQA
                     IEAKEKVEIKAENQTLATITLQNYFRLYDKLSGMTGTAQTEAAELHEIYKLGVVSIPT
                     NKPMIRADQSDLIYKTEEAKYIAVVDDVAERYDKGQPVLIGTTSVERSEYLSRQFQKR
                     RIPHNVLNAKYHEQEAGIIAEAGRRGAITVATNMA"
BASE COUNT          469 a          747 c          676 g          318 t
ORIGIN      
        1 aagggccgca acgtcgagat ccccgaccac ttccgcatct acgtctcaga gaaactcgcc
       61 cgcctcgagc ggttggaccg cagcatctac ctcttcgatg tcgaactcga ccacgaacgc
      121 aatcggcgcc aacgcaagaa ctgccagcac gtagagatca ccgcgcgggg tcgcggaccg
      181 gtcgtgcggg gcgaggcctg cgcggacagt ttctacgccg ccctcgagtc ggcagtctgc
      241 aaactcgaga accggctgcg ccggagcaag gaccgccgca aggttcacta cggcgacaag
      301 accccggtgt cactggccga ggccaccaag gtcgcggcgc ccgaggcgtt cgacccgacc
      361 ggcgctgacg acggcgcgcc gcacgacggc gtcgagatcc atgagcccgg tcagatcgtg
      421 cgcaccaagg aacacccggc caagccgatg accgtcgacg atgcgctcta cgagatggaa
      481 ctggtcggcc acgacttctt cctgttccac gacaagcaga gcgacaggcc gtgcgtggtg
      541 tatcgcaggc acgcctacga ctacggcctg attcggctcg cctgagccgg aagcctgatt
      601 cggctcgcct gagccggaag cctgatcggc tcgcctgagc cggaaacccg attcggctgg
      661 cctgaggtgc aaataaccag ccacttcggc gtgtcaccta ggatggaccc cgactaatac
      721 gcgacgggat acatcaaccc actaggggaa actagcgtgc tcgataagtt gctgcgtttc
      781 ggcgaaggcc gcatggtcaa gcgcctcaag cgggtgtctg actatgtcaa caccttgtcc
      841 gacgacgtcg aaaagctctc cgacaccgaa ctgcgcgcca agaccgatga gttcaagaag
      901 cggtacgccg atggcgaaga tctcgacgac ctgatgccgg aggcattcgc cgtggcccgc
      961 gaggcggcat ggcgcgtgct cgaccagcgg ccctacgacg tgcaggtgat gggcggagcc
     1021 gccctgcact tcggcaacgt cgccgagatg aagaccggcg agggcaagac gctgacctgt
     1081 gtgctgcccg cctacctcaa cgcactgtcc ggcaagggcg tgcacgtcgt caccgtcaac
     1141 gactacctgg ccaagcgcga cagcgagtgg atgggccggg tgcaccgcct cctcggcctc
     1201 gaggtcggcg tcatcctcgc ccagatgacg cccgaccagc gtcgcgtcgc ctacaacgcc
     1261 gacatcacct acggcaccaa caacgagttc ggattcgact acctgcgcga caacatggcg
     1321 cactcctcgg aggagatggt gcagcgcggg cacgcgttcg gcatcgtcga cgaggtcgac
     1381 tccatcctga tcgacgaagc ccgcacgccg ctgatcatct ccggtcccgc cgacggatcg
     1441 tcgaactggt actccgagtt cgcccggctg gccccgctga tggagaagga caagcactac
     1501 gaggtcgaca tccgcaagcg caccatcggt gtcagcgagc tcggcgtcga gttcgtcgag
     1561 gaccagatcg gcatcgacaa cctctacgag gccgccaact cgccgctggt cagctacctc
     1621 aacaacgcca tcaaggccaa agagctgttc atgcgcgaca aggactacat cgtgcgcgac
     1681 ggcgaggtgc tgatcgtcga cgagttcacc ggccgtgtgc tcctcggccg ccgctacaac
     1741 gagggcatgc accaggccat cgaggccaag gaaaaggtcg agatcaaggc cgagaaccag
     1801 acgctggcca ccatcacgct gcagaactac ttccggctct acgacaagct ctcaggcatg
     1861 accggcaccg cccagaccga ggcggccgag ctgcacgaga tctacaagct gggcgtggta
     1921 tccatcccga ccaacaagcc gatgatccgc gccgaccaat ccgacctgat ctacaagacc
     1981 gaagaggcca agtacatcgc ggtcgtcgac gacgtcgccg agcgctacga caagggccag
     2041 ccggtgctga tcggcaccac cagcgtggag cgttcggaat acctgtcgcg gcagttccag
     2101 aagcggcgga tcccgcacaa cgtgctcaac gcgaagtacc acgagcagga ggccggcatc
     2161 atcgccgagg ccggcaggcg gggcgcgatc accgttgcca ccaacatggc
//
LOCUS       DASYLX010000050         6012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2648, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000050 DASYLX010000000
VERSION     DASYLX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGP27_01230"
     CDS             <1..1062
                     /locus_tag="VGP27_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEV7849971.1"
                     /translation="YREALTKLQREAPPLPAAKVHRVLDGQLGTKWRERFSSFEDKPV
                     ASASIGQVHKAVWSDGREVAVKIQYPGADEALRADLKTMQRMVKVLKQISPGADVQGV
                     VDELVERTEMELDYRLEADNQRAFAKAYEGHPHFVVPHIVASAPKVVIQEWIEGIPMS
                     VIIREGTQEQRDLMGSRLFELTYDAPRRLQMMHGDAHPGNFMLLPDDKMGVIDFGAVA
                     PLPGGIPPELGMATRFALNRDYENLLPTMEKIGFIQKGEQVSTRELDDMLRQYVEPLE
                     VEVFHYTRKWLQKMTAVNIDRAAGQIRAARQMDIPAKLAIPMRVIASNVAISCQLDAY
                     IPTRRLATELVPGFAEEAA"
     gene            complement(1111..1383)
                     /locus_tag="VGP27_01235"
     CDS             complement(1111..1383)
                     /locus_tag="VGP27_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7849972.1"
                     /translation="MSGPLTCRKRTLPAVPCHVGDPDLWFAESPVDLERAKTLCAACP
                     IRRECLIAALDRNEPWGVWGGEILERGTIVARKRPRGRPRKEPAAA"
     gene            complement(1518..1685)
                     /locus_tag="VGP27_01240"
     CDS             complement(1518..1685)
                     /locus_tag="VGP27_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849973.1"
                     /translation="MANINTFTGVSIAGMAQTLHVPAIGAAAHSAAAAITPRKPRTAT
                     AIVASVNRGNI"
     gene            complement(1790..3916)
                     /locus_tag="VGP27_01245"
     CDS             complement(1790..3916)
                     /locus_tag="VGP27_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEV7849974.1"
                     /translation="MSVDSARTRSDSDATHLDELDDEQREAVLAARGPVCVLAGAGTG
                     KTRTITRRIAHLVTSGHVAAGQVLAVTFTQRAAGEMRSRLRALDDGSGTGSVQAMTFH
                     AAARRQLQYFWPRVVGDTGWQLLDSKFSVVAQAASRAGMQVSTDDVRDLAGEIEWAKA
                     SLISPEDYPATVAKVGRDIPLDAAKVAGVYAGYETLKARGDGVALVDFDDLLLHTAAA
                     IENDAAVAEEFRDRYRCFVVDEYQDVTPLQQRVLNAWLGERDDLTVVGDANQTIYSFT
                     GATPRYLLDFSRRFPEAAVVRLERDYRSTPQVVSLANQVIAAARGRVAGSKLHLIGQR
                     DPGPKPSFAEHSDEVAEAAAVAKKITRLIENGTAPSEIAVLYRINAQSEVYEEALTEA
                     GVAFQVRGGEGFFSRQEVRQALLAMQRAAERGADAPGDELPPLVRQLLEPLGLTAEPP
                     SGTRARERWEALTALAELVDDEVTQRPSLDMRALVAELRQRADARHPPVVQGVTLASL
                     HAAKGLEWDAVFLVGLADGTLPISHALAHGPESEAVEEERRLLYVGITRARVHLTLSW
                     ALARNPGGRQGRRPSRFLNGIAPNSHADAPNKPRKPRGPTPRCRVCNNALTTPAAVML
                     RRCEGCPSDLDEQLLADLKDWRLRTSKEMSVPAYVVFTDNTLIAIAETLPTDEAALVS
                     IPGIGARKLEQFGPDVIALVKGRQSP"
     gene            4016..4264
                     /gene="mrx1"
                     /locus_tag="VGP27_01250"
     CDS             4016..4264
                     /gene="mrx1"
                     /locus_tag="VGP27_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin Mrx1"
                     /protein_id="HEV7849975.1"
                     /translation="MTASDAQLTMYTTSWCGYCFRLKTALKAEGIGYTEVDIESDVAA
                     AEFVMSVNAGNQTVPTLKFPDGSTLTNPSARDVKAKLR"
     gene            complement(4378..5301)
                     /gene="nudC"
                     /locus_tag="VGP27_01255"
     CDS             complement(4378..5301)
                     /gene="nudC"
                     /locus_tag="VGP27_01255"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEV7849976.1"
                     /translation="MPPFQLRNVPLLSRVGADRADALRTDIDAAVAGWPDALLLRVDS
                     RNQVLISDGQVVLGKATALGNKPTDHAVFLGRMTDGRHVWAVRSALVAPEGSGETEVL
                     DLRRAGHIFDDVSAQLVATATALLNWHDSARFSAADGSPTKLTKGGWSRVNPVNGHEE
                     FPRIDPAVICLVHDGHDRAVLARQTMWPERLFSLLAGFVEAGESFEACVAREIAEEIG
                     LTVSDVTYLGSQPWPFPRSLMVGFHAIGDPEQEFAFLDGEIAEAAWFTRAEIREALDQ
                     GDWSSDSPSRLLLPGSISIAREIIESWAALD"
     gene            complement(5310..>6012)
                     /locus_tag="VGP27_01260"
     CDS             complement(5310..>6012)
                     /locus_tag="VGP27_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEV7849977.1"
                     /translation="VETLTTQSRQALKIQRWRSRVRNHTIVVGYGTKGRTAVAAMVGD
                     EVAPADIVVVDEDPAALERAKSAGLVTVRGSATDSDVLRLASAQHAKAIIVATNRDDA
                     AVLVTLTARELAPNAKIIAAVREAENQHLLEQSGADSTVVTSETAGRLLGIAVQTPSV
                     VEMVEDLLTPDAGFAIAEREVEPKEVGGSPRHNTDIVLGVVRNGKLLRVDDPQVDALE
                     LGDRLLYIRSVEPER"
BASE COUNT          953 a         2053 c         1995 g         1011 t
ORIGIN      
        1 taccgcgagg cgctgaccaa gctgcagcgc gaagccccgc cgctgcccgc cgccaaggtg
       61 caccgcgtgc tggacggtca gctgggtacg aaatggcgcg aacggttctc gtcgttcgag
      121 gacaagcccg tggcgtcggc cagcatcggt caggtgcaca aggcggtgtg gtccgacggg
      181 cgcgaggtcg cggtcaagat ccagtacccc ggcgccgacg aggcgctgcg cgccgacctg
      241 aagaccatgc agcggatggt caaggtgctc aagcagatct cccccggcgc cgatgtgcag
      301 ggggtcgtcg acgagttggt cgagcgcacc gagatggagc tcgactaccg actggaggcc
      361 gacaaccagc gcgcgttcgc caaagcctat gagggacatc cgcatttcgt ggtgccgcac
      421 atcgtggcca gcgcgcccaa ggtcgtcatc caggagtgga tcgagggcat cccgatgtcc
      481 gtcatcatcc gcgagggcac gcaggagcag cgcgacctca tgggctccag gttgttcgag
      541 ctgacctacg acgcgccgcg gcggctgcag atgatgcacg gcgacgcgca cccgggcaac
      601 ttcatgctgc tgcccgacga caagatgggc gtcatcgact tcggcgccgt ggcgccgctg
      661 cccgggggca ttccgcccga actgggcatg gcgacgcgct tcgcgctcaa cagggattac
      721 gaaaacctgc tgccgaccat ggagaagatc ggcttcattc agaagggcga gcaggtgtcg
      781 acccgcgaac tcgacgacat gctgcgccaa tacgtggagc ccctcgaggt ggaggttttc
      841 cactacaccc gtaagtggct acagaagatg accgccgtca acatcgatcg cgcggccggc
      901 cagatcaggg ccgcgcgcca gatggatatt cccgccaagc tggcgatccc gatgcgggtg
      961 atcgcctcga acgtcgcgat ctcctgccag ctcgacgcct acattccgac gagaaggctt
     1021 gccaccgaac tggttccggg cttcgccgag gaagcggctt aggccttagc gcttcccgct
     1081 cttgcgagac gccggaaacc ggcagccgtt tcaggcggct gccggttcct tgcgtggacg
     1141 cccacgtggc cgcttgcgcg cgacgatcgt gccgcgttcg aggatctccc caccccaaac
     1201 gccccacggc tcgttgcggt ccagcgccgc gatcaggcac tcccgtcgaa tcgggcaggc
     1261 tgcgcacagt gtcttggcac gctcgagatc gacggggctt tccgcgaacc acaagtcggg
     1321 atcgccgaca tggcacggca cagccggtag cgtcctcttt cggcatgtca gagggccaga
     1381 catgtccgtt cacctacttc ctggtcgttg atcttcgttt tccttggatc tgtgaccagg
     1441 tgttcggggg tgtcccaaaa actttggcca cggatccggt gatttcgggt ccgtggccat
     1501 tcggctttct gggcttgcta gatgttgccc cgattcacgg acgcgacaat cgcggtggcg
     1561 gtacgcggct tacgcggcgt aatcgcagcg gcagcggaat gcgccgcggc accgattgcc
     1621 gggacatgga gggtctgcgc catgccggca atgcttacgc cggtgaatgt gttgatgttc
     1681 gccatgatcg cagcaaccct cctctcgtcc gctcgggtgt gatcttgagg ctaagcctag
     1741 cagccaatta ccacaaccaa ttttctgacc tgcggttttg gcgcgatctt tacggcgatt
     1801 ggcggccttt gaccaacgcg atcacgtcgg gtccgaactg ctcgagcttg cgggcgccga
     1861 tgccgggtat cgagacgagc gcggcctcgt cggtcggcag cgtttcggcg atcgcgatca
     1921 acgtgttgtc ggtgaacacc acatacgcgg gcacgctcat ctccttggag gtgcgcagcc
     1981 gccagtcctt gaggtcggcg agcagttgct cgtcgaggtc cgacgggcag ccttcgcagc
     2041 ggcgcaacat gacggccgcc ggtgtggtca gcgcgttgtt gcagacgcgg caccgcgggg
     2101 tcgggccgcg cggctttcgc ggcttgttcg gggcgtcggc gtgcgagttc ggggcgatgc
     2161 cgttgaggaa gcgcgacgga cgccgcccct gcctgccccc cggattgcgc gccaacgccc
     2221 agctgagcgt caaatgcact cgggcccttg tgattccgac gtacagcagc ctgcgctctt
     2281 cttcgacggc ctcgctctcg ggtccgtgcg ccagcgcatg cgagatgggc agggtcccgt
     2341 ccgccagacc gacgaggaac acggcgtccc actccagacc cttcgccgca tgcagcgagg
     2401 ccagggtgac gccctgcacg acgggtgggt gccgcgcgtc ggcgcgctgc cgaagctcgg
     2461 cgaccagcgc acgcatatcg agcgaagggc gctgcgtcac ttcatcgtcg acgagctcgg
     2521 cgagcgctgt cagcgcctcc caccgctcgc gggcccgcgt gcccgacggt ggttcggccg
     2581 tcaatccgag cggttcgagc aactggcgca ccaagggcgg caattcatcg ccgggcgcgt
     2641 cggcgccgcg ttccgccgcg cgctgcatcg ccagcagcgc ctgccggacc tcctgacggc
     2701 tgaagaaccc ctccccgccg cgtacctgga acgcgacgcc cgcttcggtc agcgcctcct
     2761 cgtacacctc cgactgggcg ttgatccggt acagcacagc gatttccgac ggcgcagtgc
     2821 cgttctcgat gagacgggtg atcttcttcg cgaccgccgc ggcctcggcg acctcgtcgg
     2881 aatgctcagc gaaggacggc ttcggccccg gatcccgctg accgatcagg tgcagcttgc
     2941 tgcccgccac ccggccgcgg gccgcggcga tcacctggtt ggccagcgac accacctgcg
     3001 gggtcgaccg gtagtcgcgt tccagccgca ccaccgcggc ctcgggaaag cgccgcgaga
     3061 aatcgagcag ataccgcggg gtggcgccgg tgaacgagta gatggtctgg ttggcgtcgc
     3121 cgaccaccgt caggtcgtcg cgctcaccga gccaagcgtt gagcacccgc tgctgcagcg
     3181 gggtgacgtc ctggtactcg tcgacgacga agcagcggta gcggtcgcgg aactcctcgg
     3241 ccaccgccgc gtcgttctcg atcgcggccg ccgtgtgcag caggaggtcg tcgaaatcca
     3301 ccagcgccac gccatcgccg cgcgccttga gcgtttcgta tccggcatag acgcccgcta
     3361 ccttggcggc gtcgagtggg atgtcacggc ccaccttggc gacggtggcc ggatagtcct
     3421 cggggctgat cagcgacgcc ttcgcccatt cgatttcgcc cgccaggtcg cgcacgtcgt
     3481 cggtgctgac ctgcatcccc gcccggctgg cggcctgggc gaccacggag aacttactgt
     3541 caagcagttg ccagccggtg tcaccgacca cccgcggcca gaagtactgc agctgacgac
     3601 gggccgcggc gtgaaacgtc atcgcctgca cggatccggt gccggacccg tcgtccagcg
     3661 cgcgcagccg gctacgcatt tcaccggctg cgcgctgggt gaacgtcact gccaacacct
     3721 ggcccgcggc gacatgtccg gatgtcacca aatgcgcgat gcgccgggtg atggtcctgg
     3781 tcttgccggt gcccgcaccg gcgaggacac acaccggccc acgcgcggcc agcaccgcct
     3841 cgcgctgctc gtcgtccagc tcgtcgagat gcgtggcgtc tgagtcgctc ctcgtgcgcg
     3901 cggagtcgac agacatgggc tccatcatgt cagtgcgcac cgacattgac gggacctgcg
     3961 agccgcgtgg cccggattac agaatttcgt cgggctcgga tacgttgaag tcgttatgac
     4021 agcaagcgac gcccagctca ccatgtacac cacgtcgtgg tgtggctact gtttcaggct
     4081 caagaccgcg ctgaaggccg aaggcatcgg ctataccgag gtcgacatcg aaagcgacgt
     4141 ggccgctgcg gaattcgtga tgtccgtaaa cgcaggcaac cagaccgtgc cgacactgaa
     4201 gttccccgac ggttccaccc tgacgaatcc gagcgcacgc gacgtcaagg cgaaactgcg
     4261 ctagtcggtg tcccctgccg gcgcgtgggc ccgcgccgag atggacgaaa tggcgcgctc
     4321 ttctcgcact tctccgccca aatgtgagtt tgggcgtcag tgtgcccagt cagtacgtca
     4381 gtccagtgcc gcccaggact cgatgatctc cctggcgatc gagatggacc cgggcagcag
     4441 cagccgcgac ggtgagtcgg agctccagtc gccctgatcg agcgcttcgc ggatctcggc
     4501 gcgggtgaac cacgcggctt cggcgatctc gccgtcgagg aacgcgaact cctgctcggg
     4561 gtcgccgatc gcgtggaagc ccaccatcag cgagcgcgga aacggccacg gctggctgcc
     4621 cagataggtg acgtcgctga ccgtcagccc gatctcctcg gcgatctcgc gggcgacgca
     4681 cgcctcgaac gactcaccgg cctcgacgaa tccggccagc agcgagaaca gccgctcggg
     4741 ccacatggtt tgccgggcca gcaccgcgcg gtcgtgcccg tcgtggacaa ggcagatcac
     4801 cgcggggtcg atccgcggga actcctcgtg tccgttgacc gggttgaccc gcgaccagcc
     4861 gcccttggtc agcttcgtcg gcgacccgtc ggcggcgctg aatcgcgcgc tgtcgtgcca
     4921 gttcagcagc gcggtcgcgg tcgccaccag ctgcgcgctg acgtcgtcga agatgtgccc
     4981 cgcgcgacgc aggtcgagga cttctgtctc gccggagcct tccggggcga cgagtgcgct
     5041 gcgcaccgcc cagacatgcc tgccgtcggt catccggccc aggaacaccg cgtggtcggt
     5101 gggcttgttc cccagtgcgg tggccttgcc gaggaccacc tgcccgtcgg agatcagtac
     5161 ctggttgcgc gaatcaaccc gcagcagaag ggcatccggc caaccggcta ccgccgcgtc
     5221 gatgtcggtg cgcagcgcgt cggcacggtc ggcaccgacg cgggacagca acggcacatt
     5281 gcgcagctga aaaggcggca tccggtcgct caccgctcgg gttccacact gcggatgtac
     5341 agcagccggt cgccgagctc gagcgcatcg acctgaggat cgtcgacccg cagcagcttc
     5401 ccgttgcgca ccacgccgag cacgatgtcg gtgttgtgcc gcggggagcc gccgacctct
     5461 ttgggctcga cctcacgctc ggcgatcgcg aatccggcgt cgggtgtcaa caggtcttcc
     5521 accatctcca caacgctggg tgtctgcacc gctatgccca gcagccggcc cgccgtctcg
     5581 gaggtcacca cggtcgagtc cgcccccgac tgctccagca gatgctggtt ctcggcttcc
     5641 ctgaccgccg cgatgatctt ggcgttgggc gccaactcgc gcgcggtgag cgtgacgagc
     5701 acggctgcgt cgtcgcggtt ggtggcgacg atgatggcct tggcgtgctg ggcgctggcc
     5761 aggcgcagca cgtccgagtc ggtcgcactg ccgcggacgg tgaccagccc cgcgctcttg
     5821 gcgcgctcga gggccgcggg gtcctcgtcg acgacgacga tgtcggcggg tgcgacctcg
     5881 tcgccgacca tcgcggcgac cgccgtcctg cccttggtgc cgtacccgac gacgatggtg
     5941 tggttgcgca ctctgctcct ccatcgctgg atcttcaatg cctggcgcga ctgcgtggtg
     6001 agggtctcga ca
//
LOCUS       DASYLX010000051        10080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1319135,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000051 DASYLX010000000
VERSION     DASYLX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319135"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGP27_01265"
     CDS             <1..187
                     /locus_tag="VGP27_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7849978.1"
                     /translation="GCRLWLDAPDNRLRCPCHSTSFSLAGETLAHQLPIAPAPLPRIQ
                     VRETNGVIEVYAPTRQA"
     gene            complement(222..938)
                     /locus_tag="VGP27_01270"
     CDS             complement(222..938)
                     /locus_tag="VGP27_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849979.1"
                     /translation="MAQTSPAGDVVQLAAGHVGDTVAQLERRIYARFGERGLTKAARD
                     LGHLVDLVESEADESRVRLRRATLAARATSIVIVALTLALLVFSLRAAVTDGLAKTAD
                     WVPLTESAINDIVFAAIAVVFLWVLPERMERRALLRLLHRLRSLAHVIDMHQLSKDPE
                     QVSPNYTPTAESVGHGLNAEQLRHYLGYCSEMLSLVAKTAALSAEHSTDGVVLETISG
                     IETLTTELSSKIWQKISLLP"
     gene            1023..1853
                     /locus_tag="VGP27_01275"
     CDS             1023..1853
                     /locus_tag="VGP27_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7849980.1"
                     /translation="MDHRRRELAISVLGGPTTVIDIAGLRVVIDPTFDPPGQHAYLTK
                     TASPAVTSAALGDIDVVLISHDEHPDNLDAEGRRVALAAPLLLTHPRAASRLGPAARG
                     LEPWQSIVLAGGAGSGSLTLQAVPAVHGPADGQRNDTGHVNCEVTGFVLSGPDVPTVY
                     LSGDNASIGAVKAITERVGAVDVAVIFAGAARVATKEHGRPLTFTGQRAAAAAELLGA
                     TAVIPAHVDGWAHFSEGRDDVVAAFEEAGLGHVITAAPNGYWIDVRWDGTSWAAVARD
                     "
     gene            complement(1810..3228)
                     /locus_tag="VGP27_01280"
     CDS             complement(1810..3228)
                     /locus_tag="VGP27_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEV7849981.1"
                     /translation="MSTPTAARTRYTVEALADWVVRSATADMGACTPMLMRRNVLDSL
                     GCAIAALDGETVRAVRDHIAGDGGPATLIGGGRSTVDQATLFNSVAVRYVDLLDTYLT
                     PGGLCHPADNFGAILAVAESAGATGDEFVLALAVAYEVQCRFSAAVPVMARGLNHALQ
                     LAVSVAAGAAKLSHLSTEQTANAIAIAAADNVSLAAIHSEPVSNWKGISPGITAQRAV
                     YTTGLAQRGITGPRGLFEGPNGLEQLFGQHVDLRLGDTALNVVNHTQLKKYCSLIHGQ
                     APIETVLALAREHGIHHGDIESVDVEVFQTAYDIAGGGSFGDKDSPSTKEQADYNLKY
                     LVAAALIDGQVGPEQLQTERIGRADVQGLLHRIDVRPNATFTAGYPRETPVRIGVTLR
                     GGQRFSREQHDFEGAPGRPLTWGRTVEKFHWLTEQYAPYALRDEIVDTVEHLGAEPIS
                     ELTRLLGDVSPVRRLPKTCRPI"
     gene            complement(3225..3578)
                     /locus_tag="VGP27_01285"
     CDS             complement(3225..3578)
                     /locus_tag="VGP27_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849982.1"
                     /translation="MNESETVLRGVLDQWKAAVGANQPQQVAALFTEDAIFQGLRPYS
                     VGRRGIADYYDSQPIGLAAEYRILETRQLADDLVLGYLSVDFSFVDRPTVTVSLGVVV
                     KCVDDDWFITYYQVS"
     gene            complement(3581..4294)
                     /locus_tag="VGP27_01290"
     CDS             complement(3581..4294)
                     /locus_tag="VGP27_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849983.1"
                     /translation="MNSVQKVAVITGASQGIGAQLVTAYRKLGYAVVANSRSIGESDD
                     PDVLTVAGDLAEPGVGARVVEAALARFGRIDTLINNAGIFIAKPFTAYTDEDFDAVTG
                     VNLRGFFEISRGAIAAMLDGERGGHVVNISTSLVDHANSQVPSALASLTKGGLNAVTK
                     SLAVEYAGRGIRANTVALGIIRTPMHPESTHDALAAMHPLGRMGDVEDVVNAVVYLEN
                     APFVTGEILHVDGGQSAGH"
     gene            4479..5348
                     /locus_tag="VGP27_01295"
     CDS             4479..5348
                     /locus_tag="VGP27_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7849984.1"
                     /translation="MNFGRAADRLHIAGPSLSQQIKALERDLKVSLFDRDRRSVALTV
                     AGATLLPGARALLDKADTLRRAAAGLSDSEPVRLGYVNWRPADLSERTAGIAQLRIDT
                     WVMPSHAQATRVAEGSLDVAICWAQTSDLAAHALDARLIGADRLYAVCVGSDTSAVEA
                     KDTLVLVDADTASWSSWNRYGEQFAHDSGAGLVRIDDGGITGPTFFEHVRRLRRPVLN
                     SPKGQTTPVPADLVARPVIHPAPYWTWSLVSRHDEGRATVLAVIEALSGGIVPLDLGD
                     DAWLPADDPHRAS"
     gene            complement(5358..7655)
                     /gene="aceA"
                     /locus_tag="VGP27_01300"
     CDS             complement(5358..7655)
                     /gene="aceA"
                     /locus_tag="VGP27_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HEV7849985.1"
                     /translation="MAIVEADAAPQTPFEQDVAAVQRYFDSPRFDGITRLYSARQVVE
                     QRGTIPTDYPVARLAAAAFYDRLRELFAQRKSITTFGPYSPGQAVTMKRMGIEGIYLG
                     GWATSAKGSTSEDPGPDLASYPLSQVPDEAAGLVRALLTADRNQQYLRLRMTDDQRAA
                     TPAVDYRPFIIADADTGHGGDPHVRNLIRRFVEAGVPGYHIEDQRPGTKKCGHQGGKV
                     LVPSDEQIKRLNTARFQLDIMGVPGIIVARTDAEAANLIDSRADERDQPFLLGATNLR
                     IPSYRSCFLALVRSFYESGLTHLNGHLLYALPEGEYAAATSWLDRQGILALVVEAVST
                     WRQDGDEAVDTLFDKVESRFVEAWQNDAGLETYGEAVADLLEFREREGESLDMSAAEW
                     RTFAERASLYEAREKAKELGADAGWDCERVKTPEGYYQLRGGIPYAIAKSLAAAPFAD
                     ILWMETKTADLADAKQFADAVHAVYPDKMLAYNLSPSFNWDTTGMSDDEMRRFPEEIG
                     KMGFVFNFMTYGGHQIDGVAAEEFATALKQDGMLSLARLQRKMRLVESPYRTPQTLVG
                     GPRSDAALAASSGRTATTKSMGAGSTQHQHLVQTEVPKKLLEDWLALWSEHYQLGEKL
                     RVQLRPLRAGGEVLELGIYGERDGGEEKLANVLVDPIKDRNGRSILTVRDQNTFAEKL
                     RQKRLMTLVHLWLVHRFKAEAVYYVTPTEDNIYQTDKMKSHGIFRDVQKDVGEIIVAE
                     VNQPRIEELLASDGTALQRLIRKED"
     gene            complement(7715..8437)
                     /locus_tag="VGP27_01305"
     CDS             complement(7715..8437)
                     /locus_tag="VGP27_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7849986.1"
                     /translation="MTPLRGQTVVLIGGSAGIGLETARRARLEGAEVILTGRSPDRLR
                     QAASEVDARSTAAFDATDTASLQKFFRDLVAPIDHVMVTAGGPSYVPLLDMTAEQVRD
                     ALSDHVVLDLEVARAAAGSMRPDGSLVFMGGTGGRRVDHRVGIVSAATAALPPFTAAL
                     ALELAPTRVNLIAAGFVDTPLSANLLGDALEDRRNELRTTLPIGRVVTAEDVAALAVH
                     LMVNTALTGATYDIDGGQQFTP"
     gene            complement(8493..8879)
                     /locus_tag="VGP27_01310"
     CDS             complement(8493..8879)
                     /locus_tag="VGP27_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849987.1"
                     /translation="MSEFFPYRFDGRFVPMWLGAGALPWRDGVTLTDDGRFVATFGLL
                     RLDTPLENISDAHVAGPYRWWTALGPRLSFADDGLTFGTNPQAGLCIHFRDKVKRVIG
                     FRDHSALTVTVADPHALEARLRSGRQ"
     gene            9058..9597
                     /gene="ripD"
                     /locus_tag="VGP27_01315"
     CDS             9058..9597
                     /gene="ripD"
                     /locus_tag="VGP27_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan-binding protein
                     RipD"
                     /protein_id="HEV7849988.1"
                     /translation="MKRIYALVVGLAVLVATPGLANADITRPLSSQQAIQYVIARGLS
                     QRGVPFSFGGGDSNGPTRGSGPADNAVGLDHQGRVVGLDPMASTVGFDASGLMLYSYA
                     GVGIKLPRSSGAQYNAGRKILPQQAVPGDLIFYGPNGNQSVAMFLGNGQMLEAGDPVV
                     TVSPVRTSNMAPYLVRVIA"
     gene            9760..>10080
                     /locus_tag="VGP27_01320"
     CDS             9760..>10080
                     /locus_tag="VGP27_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV7849989.1"
                     /translation="MAVLTPAQQDFPRWYQDVLTKAKLAENGPARGTMVIRPNGYAIW
                     ERMADEVDRRIKAAGADNAYFPLLMPMSFFEREAEHVEGFSPELAVVTHGGGKELAEP
                     LAIRP"
BASE COUNT         1635 a         3300 c         3388 g         1757 t
ORIGIN      
        1 aggctgccgg ctgtggctcg atgcgccgga caaccggctg cgttgcccgt gccactcgac
       61 gtcgttctcg cttgccgggg agacgctggc ccatcagctg ccgatcgctc ctgcgccgtt
      121 gccgcgcatc caggtccgcg aaaccaatgg ggtgatcgag gtgtacgcgc cgacccgaca
      181 agcctgaatc gagttgcgca tcatccgatc ggcgtgacga gttaggggag gagcgagatc
      241 ttctgccaga tcttgctgga cagctcggtg gtgagggtct cgatgccgga gatggtctcg
      301 agcacgacgc catcggtgct gtgctcggcg ctcaacgccg ccgtcttggc cacgaggctc
      361 agcatctccg agcagtagcc gaggtaatgg cgcaactgct cggcgttcag gccgtgcccg
      421 acgctctctg cggtcggcgt gtagttcggg ctgacctgct ccggatcctt ggagagttgg
      481 tgcatgtcga tgacgtgggc cagcgagcgc agccgatgga gcagccgaag caatgcgcgc
      541 cgctccatgc gctcgggcaa gacccagagg aacacgaccg cgatcgccgc gaagacgatg
      601 tcgttgatgg cgctctcggt cagtggcacc cagtctgcgg tcttggcgag cccgtcggtg
      661 accgccgcac gcaggctgaa caccagcagg gccagcgtca gcgccacgat cacgatgctc
      721 gtggcgcggg ctgccagtgt cgcgcgtcga agacgcaccc gcgactcgtc agcctcgctc
      781 tcgacgaggt cgaccagatg gccgaggtcg cgcgccgcct tggtcagtcc tcgctccccg
      841 aagcgggcgt agatgcgtcg ttcgagctgc gccacggtgt ccccgacgtg gccagcggcg
      901 agctgcacca cgtctcctgc tgggctcgtc tgcgccacgc aacgactgta gaagtaatcg
      961 ggccgctgat ggcgaactat ggtcggttca gacaggcgcg aaggagcgac atggacgagt
     1021 tggtggacca ccgacgacgt gaactggcca tcagcgtgct tggcgggccc accaccgtca
     1081 tcgacatcgc cgggctgagg gtggtcatcg accccacctt cgacccgcca ggacaacacg
     1141 catacctgac gaagaccgcg agcccggccg tcacctcggc ggcacttggc gacatcgacg
     1201 tcgtgctgat cagtcacgac gagcaccccg acaatctcga tgcggagggc cgtcgggtgg
     1261 cgctcgccgc accgcttctt ctcacacacc cgcgcgcagc aagccggttg gggccggcgg
     1321 cccgaggtct cgagccgtgg cagtccatcg tcctggccgg tggagccgga tcgggatccc
     1381 ttacgttgca ggcggttccg gcggtgcatg gccctgcgga cggacagcgc aacgacacgg
     1441 gccacgtcaa ctgcgaggtg acgggtttcg tgctgtccgg gcccgacgtt ccgactgtct
     1501 atctcagcgg cgacaacgca tcgatcggcg cagtcaaggc catcaccgag cgcgtcggcg
     1561 cagtcgacgt cgccgtgatc ttcgccggcg cggcgcgggt ggcgaccaag gagcacggcc
     1621 gccccctgac gttcacgggc cagcgggctg ctgcggccgc cgagttactc ggtgcgacgg
     1681 cggtcatccc ggcccacgtc gacggctggg ctcacttcag cgagggccgc gatgacgtcg
     1741 tcgccgcatt cgaggaagca gggctcggcc acgtgatcac cgcggcaccg aacgggtact
     1801 ggatcgacgt cagatgggac ggcacgtctt gggcagccgt cgcacgggac tgacgtcacc
     1861 gagaagtcgg gtcaattccg agatcggttc agcaccaagg tgttcgaccg tgtccacgat
     1921 ctcgtcgcgc aacgcatacg gcgcatattg ctctgtcagc caatggaact tctcaaccgt
     1981 ccgcccccac gtcaacggcc gcccgggtgc tccctcgaag tcgtgctgct cgcgtgagaa
     2041 gcgttggcca cctcgcaggg tcactccgat tcgaaccggt gtctcgcgcg gataacccgc
     2101 ggtgaacgtg gcgttgggcc gtacgtcgat gcgatgaagg agcccttgga cgtctgcgcg
     2161 cccgattcgc tcggtctgga gttgctcggg cccgacctgg ccgtcgatca aggcggctgc
     2221 caccaggtac ttgaggttgt agtcggcctg ctccttggtc gacggcgagt ccttgtcgcc
     2281 gaagcttcca ccgccggcga tgtcgtaggc ggtctgaaac acctcgacgt cgaccgactc
     2341 gatgtccccg tgatggatac cgtgctctcg agcaagtgcg agaacggttt cgatgggcgc
     2401 ttggccgtgg atgagcgagc agtacttctt cagctgcgtg tggttgacga cgttcaaagc
     2461 ggtgtcgccg aggcggaggt cgacgtgttg gccgaagagt tgctcgagcc cattgggtcc
     2521 ctcgaagagc ccacgcgggc cggtgattcc gcgctgcgcc aatccggtgg tgtacaccgc
     2581 acgttgggcg gtgataccgg gggagatgcc cttccagttc gataccggct cggagtggat
     2641 cgccgccaac gacacgttgt ccgccgccgc gatggcgatg gcgttggccg tctgttcggt
     2701 cgacaagtgc gacagtttgg cggcgcctgc cgcgaccgaa accgccagct gcagcgcatg
     2761 gttgagacca cgtgccatca cgggtacggc cgccgagaac cggcactgca cttcgtaggc
     2821 cacggccagc gccaggacga attcgtcgcc cgtggcacct gcgctctccg ccaccgccag
     2881 gatcgcgccg aagttgtcgg cgggatggca caagccacct ggcgtcagat acgtgtcgag
     2941 caggtcgacg taccgcaccg ccaccgagtt gaacaacgtg gcctgatcga ctgtcgatcg
     3001 accgccgccg atcaacgtcg ctgggccgcc atcccctgcg atgtggtcgc gcactgcccg
     3061 cacggtctca ccgtcgagcg cggcaatcgc acagccaagg ctgtcgagaa cgttgcgccg
     3121 catcaacatc ggcgtacaag cgcccatgtc ggccgtcgcg gaccgcacca cccaatctgc
     3181 gagagcctcc accgtgtagc gggtgcgcgc agccgtcggc gtgctcacga gacctggtag
     3241 tacgtgatga accagtcgtc gtcgacgcat tttaccacga caccaaggga gacggtcacg
     3301 gtcggccgat cgacgaacga gaagtcaacg ctcagatagc cgagcaccag atcgtcggcc
     3361 agctgccggg tttcgaggat gcggtattcg gccgccaaac cgatgggttg ggaatcgtag
     3421 tagtcggcga tgcctcggcg tcccacgcta tatgggcgca gaccttggaa gatcgcgtct
     3481 tcggtgaaca gggcggcgac ttgctggggt tggttcgcgc cgacggcggc cttccattgg
     3541 tcgagcacac cgcgcaggac ggtctcggac tcattcatga tcagtgccct gcgctctggc
     3601 cgccgtcgac gtgcaggatc tcgccggtca cgaacggggc gttctccagg tagacgacgg
     3661 cattcacgac gtcctcgacg tcacccatcc ggccgagcgg gtgcatcgcg gccagggcat
     3721 cgtgggtcga ctccgggtgc atcggcgtgc ggatgatgcc cagcgcgacg gtgttggccc
     3781 ggatgcctcg accggcgtac tcgacggcca gcgacttggt caccgcgttc agtccgccct
     3841 tcgtcaggga cgccaacgcc gaaggcactt gagagttggc gtgatcgacc aggctggtgg
     3901 agatgttcac gacgtgccca ccgcgttccc cgtcgagcat cgcggcgatc gcgccacgcg
     3961 aaatctcgaa gaagccacgc agattcactc cggtgaccgc gtcgaaatcc tcgtcggtgt
     4021 acgcggtgaa cggtttggcg atgaagattc cggcgttgtt gatcaatgtg tcgatccgac
     4081 cgaaccgtgc cagcgccgct tccacgactc gagcaccgac gcccggctcg gccaggtcgc
     4141 cggcgacggt cagtacgtcg gggtcgtccg actcgccgat cgagcgcgag ttggccacga
     4201 ccgcgtagcc aagcttgcgg tacgcggtga ccagttgggc tccgattcct tgggacgccc
     4261 cggtgatgac ggctaccttc tggactgaat tcatgtgacg cctcgcttcg ttggtgcagc
     4321 taggtgttgc ttacaccccg ttgaacgcat cgtggggccg gctgctgccc cgacaccagg
     4381 accggtttgc ttcctgctga gaggcgcacg cttccaccgc actacgattg gcccgtggag
     4441 ctgcgtcagc tgcgctattt cgtcatcctc gccgggaatt gaacttcggc cgcgcagctg
     4501 accgcctgca catcgcgggc ccttcgctct cccaacagat caaggccctc gagcgtgacc
     4561 tgaaggtgag cctgttcgac cgcgatcgtc gatccgtcgc cttgaccgtc gccggggcca
     4621 cgttgctgcc gggtgcccgc gcactgctcg acaaggccga cacgctgcgc agggccgcgg
     4681 ccggcctctc cgattccgaa cccgtccggc tcggctacgt caattggcgg cccgccgact
     4741 tgtcggagag gacggccgga attgcccagt tacgcatcga cacatgggtg atgccctcac
     4801 atgcgcaagc cacgcgagtg gccgagggca gcctcgacgt cgcgatctgc tgggctcaga
     4861 ccagcgacct ggccgcgcac gcgctcgacg cgaggctcat cggggccgac cggttgtacg
     4921 ccgtctgcgt cggctccgac acctcggcgg tcgaggcgaa ggacacgttg gtgctcgtcg
     4981 atgcggacac tgccagttgg tcgtcgtgga accgctacgg cgaacagttc gcccacgaca
     5041 gcggcgccgg tctggtgcgc atcgacgacg ggggcatcac tggcccaacg tttttcgagc
     5101 acgtgcgccg cctgcgcagg cccgtgctca actcaccgaa ggggcagacg acgccggtac
     5161 ccgcagatct ggtggcacgt ccggtgatcc accctgcgcc gtattggacg tggtctctcg
     5221 tctctcgcca tgacgaagga cgagcgacgg tgctcgccgt gatcgaggcg ctctcgggcg
     5281 gcatcgttcc gctggacctg ggcgacgacg cctggctgcc cgcagacgat ccgcatcgtg
     5341 cgtcgtgacc cgttggccta atcctccttg cggatcaacc gctgcagcgc ggtgccgtcc
     5401 gacgccagca attcctcgat gcgcggctgg ttgacctcgg cgacgatgat ctccccgacg
     5461 tccttctgta cgtcgcggaa gatgccgtgg gacttcatct tgtcggtctg atagatgttg
     5521 tcctccgtcg gcgtgacgta gtagaccgcc tcggccttga accggtgtac cagccacagg
     5581 tggaccaggg tcatcaggcg cttctggcgc agtttctcgg cgaaggtgtt ctgatcgcgc
     5641 accgtaagga tgctgcggcc gttgcggtcc ttgatcgggt cgacgagcac gttggcgagc
     5701 ttctcctcac caccgtcccg ttcaccgtag atgccgagct cgagcacttc gccgcccgcg
     5761 cgcagtggcc gcagttggac acggagcttc tcaccgagct ggtagtgctc gctccacaga
     5821 gccagccagt cctccagcag cttcttgggc acctcggtct gaacgaggtg ctgatgctgc
     5881 gtggagcccg cgcccatgga cttggtggtg gccgtacggc cggacgaggc ggccagcgcg
     5941 gcgtcgctgc gcggtccgcc gaccagggtt tgcggcgtgc gatagggcga ctcgaccagg
     6001 cgcatcttgc gttgcagccg ggcgagcgac agcatgccgt cctgtttgag tgcggtcgcg
     6061 aattcctcgg cggcgacgcc gtcgatctgg tgcccgccgt aggtcatgaa gttgaagacg
     6121 aagcccatct tgccgatttc ctctgggaac cggcgcatct cgtcgtcgct catcccggtg
     6181 gtgtcccagt tgaacgacgg ggagaggttg taggcgagca tcttgtcggg atacacggcg
     6241 tgcacggcgt cggcgaactg tttggcgtcg gcgagatccg cagtcttcgt ctccatccac
     6301 aggatgtccg cgaacggcgc tgcggcaagc gatttggcga tcgcgtacgg gattccgcca
     6361 cgcagctggt agtagccctc gggggtcttc acccgctcgc agtcccagcc tgcgtcggcg
     6421 ccgagctcct tcgccttctc acgtgcctca taaagcgacg ccctctccgc gaacgttcgc
     6481 cattccgcgg cgctcatgtc gagcgattcg ccctcgcgct cgcggaactc gagaagatcg
     6541 gcgaccgcct cgccgtaggt ctcgaggccg gcatcattct gccaagcttc gacgaagcgt
     6601 gactcgacct tgtcgaacag ggtgtcgacg gcttcgtcgc cgtcctgccg ccaggtcgac
     6661 accgcttcta cgacgagagc caggatgcct tgccggtcaa gccaactggt cgccgctgcg
     6721 tactcaccct cgggcagcgc atacagcaga tgaccgttga ggtgcgtcag ccccgattcg
     6781 tagaagctgc gcaccagcgc caggaaacag gacctgtaag acgggattcg caggttggtc
     6841 gcgcccagca ggaacggctg gtctcgttcg tcggcgcggc tgtcgataag gttggccgcc
     6901 tccgcgtcgg tgcgcgcgac gatgatgccc ggcaccccca tgatgtccag ctggaagcgg
     6961 gcggtgttga ggcgcttgat ctgttcgtcc gaaggaacca gtaccttgcc gccctgatgt
     7021 ccacacttct tggtgccggg ccgttggtcc tcgatgtggt aaccgggcac gcccgcctcg
     7081 acgaagcggc gaatcagatt gcgcacgtgc ggatctccgc cgtggccggt gtctgcgtcg
     7141 gcgatgatga acgggcggta gtcgacggca ggcgttgcgg cgcgctggtc gtcggtcatg
     7201 cgcaggcgca ggtactgctg gttgcggtcg gcggtgagca gcgcgcgcac caacccggcg
     7261 gcctcgtccg gtacctggct cagcggatag ctggcgaggt cgggaccggg gtcctcgctg
     7321 gtggacccct ttgcggaggt cgcccagcca ccgaggtaga tcccctcgat gcccatccgc
     7381 ttcatcgtca ccgcctgacc cggcgagtac ggcccgaagg tggtgatgct cttgcgctgg
     7441 gcgaacagtt cgcgcaggcg gtcgtagaag gctgccgccg ccaggcgcgc gaccgggtag
     7501 tcggtgggga tcgtcccgcg ctgctctacg acctggcggg ccgaatagag gcgggtgatc
     7561 ccgtcgaacc gggggctgtc gaaataccgc tgcacggcgg cgacatcctg ctcgaacggg
     7621 gtctggggcg ctgcatcagc ttcgacgatg gccatagccc aacactacgg cgcacgtttg
     7681 gacgatagtg cggattcaca ctgcctgcag ttcgctacgg cgtgaactgc tgaccgccgt
     7741 cgatgtcgta ggtcgcaccc gtgagggcgg tgttgaccat gagatgtacg gcgagcgccg
     7801 ccacgtcttc tgccgtcacc acgcgaccga tcggcagggt ggtccgcaac tcgttacgtc
     7861 gatcctccag cgcgtcaccg agcaggttgg ccgacagcgg ggtgtcgacg aaacccgcgg
     7921 ctatcaggtt gacgcgggtc ggggccagtt cgagcgccag ggccgcggtg aacggcggca
     7981 gcgccgcggt cgccgccgag acgatgccga cccggtggtc gacgcgcctg ccgccggtgc
     8041 cgcccatgaa gaccaggctg ccgtcgggcc gcatggagcc ggcagcggca cgggccacct
     8101 cgaggtcgag caccacgtga tcgctgagcg cgtcgcgcac ctgctctgcg gtcatgtcga
     8161 ggagcgggac gtagctgggg ccaccggccg tcaccatcac atgatcgatc ggggcgacca
     8221 ggtctctgaa gaacttctgc aaggacgccg tgtcggtggc gtcgaacgcc gcggtgcttc
     8281 gcgcatccac ttccgatgcg gcctgtcgga gccgatccgg gctgcggccg gtgaggatca
     8341 cctcggcgcc ctccagcctg gcgcgccgag ccgtctcgag cccgatcccg gcgctaccgc
     8401 cgatgagtac gacggtttgt ccgcgcaacg gtgtcaccat cgaagtgtgg cggcgcgagg
     8461 cggccgcgac aactgggcgc tcaccgacga tgctattgcc gaccgctgcg caaccgggct
     8521 tccagcgcgt gcggatctgc gacggtgacg gtcagcgcgg agtggtcacg gaagccgatg
     8581 acgcgcttga ccttgtcgcg gaaatgtatg cacaggcccg cctgcggatt cgttccgaac
     8641 gtcagcccgt cgtcggcgaa cgagagacgc gggccgagcg cggtccacca gcggtaggga
     8701 cccgcgacat gcgcatccga gatgttctcc agcggggtgt ccaaccggag caggccgaag
     8761 gtcgcgacga accggccgtc gtcggtgagc gtgacgccgt cccgccacgg cagcgcaccg
     8821 gcgccgagcc acatcggcac gaaccgcccg tcgaagcggt acggaaagaa ctcgctcacc
     8881 gcctcatgca agcacaacgg tcaccgttgc gtcacgttac ggccactcat tggcgatgtg
     8941 ccgtgcggcg catggcccct gacggcgcgg ggttgtagaa gtagctgtgg tgacatccgt
     9001 ccaccaacgg gtcacccatt gtggtcgcgg tgaaatcaaa gaaagtgaac gctggacatg
     9061 aaacgcattt acgccttggt ggtcggtctt gccgtgttgg tggcgacgcc gggtttggcg
     9121 aacgccgaca tcacccgtcc gctcagcagc cagcaggcca tccagtatgt gatcgcgcgt
     9181 ggtctctccc agcgcggtgt gccgttctcg ttcggtggcg gcgactccaa cggcccgacc
     9241 cgcggcagcg gcccggcgga caatgccgtc gggctggacc accaggggcg cgtcgtcgga
     9301 ctggatccga tggcaagcac cgtgggtttc gacgcctccg gcctgatgct gtactcctat
     9361 gccggtgtcg gaatcaagct gccgcgttcg tcgggggccc agtacaacgc cggccgcaag
     9421 attctgccgc agcaggcggt tcccggcgat ctgatcttct acggcccgaa cggaaaccag
     9481 agcgtggcga tgttccttgg caacgggcag atgctggagg caggcgaccc ggtcgtcacc
     9541 gtgtcgcccg ttcggaccag caacatggcg ccgtacctgg tgcgggtcat cgcgtgagct
     9601 gacgtaacgc cgaccccagg cgccgtcccg cgagcgctca ggtttctacg gtttcggcag
     9661 ggaaatctgt acgaacccga gcgctcggtc gcactggatt cccgtcgccg acataaaacc
     9721 gcgcgacttc ggcgggacgg catcagtaac gtcgcgcaga tggcagtgct gactcctgcg
     9781 caacaggact tcccacggtg gtatcaggac gtgctgacca aggcgaagct cgccgagaac
     9841 ggccctgccc gcgggaccat ggtgatccgg ccgaacggct acgccatctg ggagcggatg
     9901 gccgatgagg tcgacaggcg gatcaaggcc gcaggggcgg ataacgcgta cttcccgttg
     9961 ctgatgccga tgagtttctt cgagcgcgaa gccgaacacg tcgagggatt cagcccggag
    10021 ttggctgtgg tgacgcacgg cggaggcaag gagctggccg aaccgctcgc cattcgtccg
//
LOCUS       DASYLX010000052         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_222010,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000052 DASYLX010000000
VERSION     DASYLX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222010"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGP27_01325"
     CDS             complement(<1..543)
                     /locus_tag="VGP27_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7849990.1"
                     /translation="MTPRTRGAQQTPRTRGAQQTPRTRGAQQTPRTESGKRHRVVVIG
                     SGFGGLTAAQALKRSDVDVTLISKSTSHLFQPLLYPVATGILSEGEIAPTTRLILRKQ
                     KNVKVLLGDVESIDLTENIVTSKLMTMTTVTPFDSLIVAAGAQQSYFGNDQFATYAPG
                     MKTIDDALELRGRILGAFEAA"
     gene            complement(652..1380)
                     /locus_tag="VGP27_01330"
     CDS             complement(652..1380)
                     /locus_tag="VGP27_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849991.1"
                     /translation="MALATLLAITITCIGWSLWIRRVTWTCRWEVAATLNIALQGLAV
                     LLMSPLASETVGHWLHALTGKWNLEDYLGHDCYIVAASAIVYNTLGRLADDDDMQRSF
                     KQYVERPATLCIPLLLVTFSIGNGARIYAPDFFTVPTDFWLNMYWLLLCGTLIYLLGY
                     GIRATLVLRKDPRSRRIANFYLASSVFGILACAVRITTAYVPQLQTAAGSSLVWVFAC
                     LCGAGFALASAHSWRIKTKWFTKV"
     gene            complement(1418..1774)
                     /locus_tag="VGP27_01335"
     CDS             complement(1418..1774)
                     /locus_tag="VGP27_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7849992.1"
                     /translation="MAVPRTDENGRQLKALLDYLLDGDVDAKDIYDALGVSSSTYYRR
                     IKEEFYPDAEELRRVADRFGLSYPDLQVRFGLMSHEEVQIYFESAGVALATVETRRVR
                     PQRLSEMKPRLDAPPL"
     gene            1904..2497
                     /locus_tag="VGP27_01340"
     CDS             1904..2497
                     /locus_tag="VGP27_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849993.1"
                     /translation="MLIAEFASCPLRIADHETADAPREADLAKISAPLDSTLTGELDG
                     AQWYLGLAGEEILLAALGLAIARTIGEGVVPVDVAGHSGAVSLSCTSAGLATATESLA
                     VVHRSLATGPATPKLAAQPPSEIFFSYLGTEPGLRHALELRAYRADGLVQLDWWYDTR
                     SFDSYTVEELAEQLPLALIELTSEAMPAQHTPMAVGH"
BASE COUNT          496 a          903 c          933 g          526 t
ORIGIN      
        1 ggcggcctcg aacgccccga ggatgcgccc gcggagctcg agcgcgtcgt cgatcgtctt
       61 catgccgggc gcgtaggtgg cgaactggtc gttgccgaag tacgactgct gggcgcctgc
      121 ggcgacgatg aggctgtcga aaggcgtcac cgtggtcatg gtcatcagct tcgacgtgac
      181 gatgttctcg gtgagatcga tcgattcgac atctcccaga agcactttga cgtttttctg
      241 cttgcgcaga atgagtcgcg tggtcggggc gatctcgccc tcggagagga ttccggtggc
      301 caccggatag agcagcggct ggaagaggtg actggtcgac ttggagatca gagtgacgtc
      361 gacgtcgctt ctcttcaggg cctgagccgc ggtcaggcca ccgaagccgg acccgatgac
      421 gacgacccgg tggcgcttgc cggactccgt gcgcggtgtc tgttgcgctc ctcgcgtccg
      481 cggtgtctgt tgcgctcctc gcgtccgcgg tgtctgttgc gctcctcgcg tgcgcggtgt
      541 catcgcgttc gtcccccctt cggcggcgac gcaacgataa cgcgtgctgg gtgcggccgc
      601 ccgtcgttgg ctaggtgttc gccggcgctc ctacagcagt tggccgcggg atcagacctt
      661 cgtgaaccac ttcgtcttga tccgccacga atgggcggag gctagcgcga aacccgcgcc
      721 gcacagacag gcgaacaccc ataccaggga gctacccgcc gccgtctgca actgcggcac
      781 ataagcggtg gtgatccgca cggcgcaggc caagatgccg aagaccgatg aggccagata
      841 gaagttcgcg atccgccgcg accgcgggtc tttccgcagc accagggtgg cccggatgcc
      901 gtaccccagc aggtagatca gggtgccgca gagcagcagc cagtacatgt tcagccagaa
      961 gtcggtcggc accgtgaaga agtcgggagc atagattcgg gcgccgttac cgatcgagaa
     1021 ggtcacaagc agcagcggga tgcacagcgt ggccggccgc tcgacgtact gcttgaacga
     1081 ccgctgcatg tcgtcgtcgt cggccagcct gcccagtgtg ttgtagacga tcgccgatgc
     1141 ggcgacgatg tagcagtcat ggccgaggta gtcctcgagg ttccacttcc cggtgagggc
     1201 atgcagccag tgcccgacgg tttcggaggc gagcggcgac atcagcagca ccgccaaacc
     1261 ctgaagggcg atattgaggg tggcggcaac ctcccaccgg cacgtccacg tcacacgccg
     1321 gatccacagg ctccatccga tgcacgtgat cgtgatcgct agaagtgttg cgagagccat
     1381 ccgtcttgcc tttgcgctga atcggtttca gcaaactcta tagcggcggg gcgtccaggc
     1441 gaggcttcat ttcagacagc ctctgcgggc gtacgcgtcg cgtttccacg gttgccagtg
     1501 ccacgccggc agactcgaag tagatttgca cctcttcatg gctcatcagc ccgaaccgaa
     1561 cctggaggtc aggatagctg agaccgaacc ggtcggcgac ccggcgcagc tcttcggcgt
     1621 cggggtagaa ctcctccttg atgcgccggt agtacgtgct gctagacacc ccgagtgcgt
     1681 cgtagatgtc cttcgcgtcg acgtcgccgt cgagtaggta gtcgagcaag gcctttagct
     1741 ggcggccgtt ctcatctgta cgtggcaccg ccatacctta tcgcgttctc ccgcatatgg
     1801 gcagtgccgg tcggccgttg taacgattgc tgatattgac gtaggtgtca cagttttggg
     1861 agaggtctcc caatcttggg acaacgcagt tactgttagc cccatgttga ttgcagaatt
     1921 tgccagctgc ccgcttcgaa tcgccgacca cgagaccgcc gacgcccctc gagaagccga
     1981 tctcgcaaag atctcggcgc cactggacag cacgctgacc ggggaactgg atggcgcgca
     2041 gtggtatctc ggcctggcgg gcgaggaaat ccttctcgcg gcactcggtc tcgcgattgc
     2101 acggacgatc ggcgagggtg ttgttcccgt cgatgtcgcc ggccacagcg gggcggtgtc
     2161 gttgtcatgc acctcagcgg gtctcgcgac cgccacggag tcgttggcgg tcgtgcaccg
     2221 aagcctcgcc acagggcctg ccactccgaa gctcgcggcc cagccgccgt cggagatctt
     2281 cttctcgtac ctcggcacgg agcccggtct gcgccatgcg ctcgagctgc gggcataccg
     2341 ggcggacggc ctggttcagc tggactggtg gtatgacacc cgcagcttcg actcctacac
     2401 cgtcgaggag ttggccgagc agctgccgct ggcgttgatc gagctgacgt ccgaggccat
     2461 gcctgcgcag cacaccccga tggccgtcgg tcactgagtc gctagaatct gacgtatcgg
     2521 cgcagatccg gccatcaccg ggaagccttc ggaagaacag ctcaggccca gtagacccga
     2581 acgggtaggc ccgtcacagc cgcaagttga gcggcgcaca catgtgcgca agcggggtgg
     2641 taccgcggcg ttcgcgcacc ggcgcgtcgt cgtccccgtg ccatgaacga cgtttggcac
     2701 aggagacgac gcacgaccgt gaccacctat ccgaagccgg caggcggcgc gcccaacttc
     2761 ccggcgattg aatccgacgt cctggactac tgggagaacg acgacacctt ccgcgccagc
     2821 gttgcgcgcc gcgacggtgc gccggagtac gtgttcta
//
LOCUS       DASYLX010000053         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3074334,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000053 DASYLX010000000
VERSION     DASYLX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074334"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGP27_01345"
     CDS             <1..238
                     /locus_tag="VGP27_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849994.1"
                     /translation="NKDAYNADPYAFLKKEEVPTPWEFTTTLEDAQWATIDASAAAHG
                     GQAVANAHCQIEVDGQVVSQSDGPYTARCQLGNW"
     gene            complement(260..1114)
                     /locus_tag="VGP27_01350"
     CDS             complement(260..1114)
                     /locus_tag="VGP27_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849995.1"
                     /translation="MFTETQWDHEVDVVCIGAEGAVLAAALVAINADLDVYSAITEST
                     AGGSDLAAAVSYRGGDGQTTKHLAGFDYAFGGVGRAQSFWPVRSVEDIVAPRSHRRGA
                     IEPFFGAALEQWAHRCAAAPSGLVYNRVTRRQMTEMRSSTRGEKIEAAVVGSVDLTPG
                     SPALSLRTWLRTQATEAGLVPDTDVRLVKLVFEDNGVAGAVIDTPDGVLAVRARENFI
                     IGLGDIPPERTRALVSATEPVIVDVCLVSKAASRFGELEIVTAPGEDNRLFFVDASDT
                     EFQLAIGL"
     gene            complement(1119..1721)
                     /locus_tag="VGP27_01355"
     CDS             complement(1119..1721)
                     /locus_tag="VGP27_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7849996.1"
                     /translation="MAGAERSYRRHGIGAAIDVDAIRRSRDTTLFWTVLNATGTVVGG
                     IRAIGPLTSPDETHAVTEWAGQPSHPMVRKMIADRLPFGVVEMKSAWVTDDPDRSRRI
                     TASLARAGCHVLAALGVQFCMATCAYHVLARWRSSGGVVAPIPSTPYPDDRYRTKMMW
                     WDRHTVASLAAPSQTAKIFAEIANIRQRLHSSSRHATVGS"
     gene            2256..>2806
                     /locus_tag="VGP27_01360"
     CDS             2256..>2806
                     /locus_tag="VGP27_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV7849997.1"
                     /translation="MRWDGQGVSVDDGALPGLQRIGFVRSVRTPQFEGITFHEVLCKS
                     ALNKVPNASMLPFRYTVNGYRGCSHACRYCFARPTHEYLDMNCGTDFDSQVVVKTNVV
                     EVLRRELHRKSWQRETVALGTNTDPYQRAEGRYALMPGIIEALADSGTPFSILTKGTL
                     LRRDLPLIADAAKRVDVSVAVSLA"
BASE COUNT          512 a          923 c          883 g          488 t
ORIGIN      
        1 gaacaaggac gcgtacaacg ccgatccgta cgccttcctg aagaaggaag aggtgccgac
       61 gccctgggag ttcaccacga cgctcgagga cgcgcagtgg gcgaccatcg acgcgagcgc
      121 cgccgctcac ggcggacagg cggtagcgaa tgcgcattgt cagatcgagg tcgacggcca
      181 ggtggtctcg caatccgatg ggccgtatac ggcgcgctgc cagctcggca actggtgatt
      241 cgacgacggc ggtagcgcct cagagtccaa ttgccagctg gaactcggtg tcagatgcgt
      301 cgacgaaaaa cagccggttg tcctctcccg gcgccgtgac gatctcgagt tcgccgaacc
      361 ggctcgccgc cttgctgacc aggcacacgt cgacgatcac cggctcggta gcgctgacga
      421 gtgcacgggt gcgctccggt ggaatatcac ccagtccgat gatgaaattc tcccgggccc
      481 gcaccgcgag cacgccgtcc ggtgtatcga tcactgcgcc tgcgacgccg ttgtcctcga
      541 agaccagctt gaccagccgc acgtcggtgt cgggcaccag cccggcttcc gtcgcctgag
      601 ttctcagcca cgtcctcaac gacaaggcag gcgatcccgg ggtcaagtcc accgatccga
      661 cgacggccgc ctcgatcttc tcgccgcggg tgctgctccg catctcggtc atctggcgtc
      721 tggtcacccg gttgtagacg agaccactcg gtgctgctgc gcatcgatgt gcccactgct
      781 cgagcgcagc gccgaagaaa ggctcgatgg caccgcggcg gtgggagcgc ggtgccacga
      841 tgtcttcgac ggagcgcacc ggccagaagc tctgcgcgcg gccgaccccg ccgaaggcgt
      901 agtcgaagcc ggccagatgc ttcgtcgtct gcccgtcccc accccggtag ctcaccgctg
      961 cggccaggtc gctgcccccc gcggtggatt ccgtgatggc cgagtagacg tcgagatccg
     1021 cgtttatggc gacgagtgcc gcggcgagca ctgcaccctc ggcgccgatg catacgacgt
     1081 cgacctcgtg gtcccactgt gtttcggtga acatggcctc aacttcccac tgtcgcatgc
     1141 cggctcgagc tgtgtagacg ctggcgaatg tttgcgattt cggcgaagat cttggcggtc
     1201 tgactcggtg cggcgaggct ggcgacggtg tgcctgtccc accacatcat cttggtccgg
     1261 tatcggtcat cgggatacgg cgtagacggg atcggagcca ccacgccgcc cgacgaccgc
     1321 cagcgcgcca gaacgtgata cgcgcaggtg gccatgcaga actgaacgcc gagcgctgcg
     1381 agcacgtgac agcctgcgcg ggccagcgac gcggtgatcc tgcggctgcg gtccggatcg
     1441 tcggtcaccc acgccgactt catttccacg acaccgaacg ggagccggtc ggcgatcatc
     1501 ttccggacca tcgggtggct tggttggcct gcccactcgg tgaccgcgtg tgtctcgtcg
     1561 ggtgatgtca gcggaccgat ggcgcggatg ccaccgacca ccgttcccgt tgcgttgagc
     1621 accgtccaga aaagcgtggt gtcgcgagaa cggcggatcg catccacgtc gatcgccgcg
     1681 ccaatgccgt ggcgtcgata cgaacgctcg gcgcccgcca aggtaccgct gccacagatc
     1741 gtcgtcgtct gaaggtctgg cgatggctac ggtgcattcg gcgtcggagt cccaccacct
     1801 catcagtgcg ccgtcgacgt tggtgggggc gaacgcgcgg ggcagttcga cgacggtcat
     1861 ggtgtctccc atcaggctga aaacgtcacc gcatccggcg cgtgtgctac tcgcacaaac
     1921 gcaaagcggt gcagatctag caatctaaca agcaaaccta gacagtgcca atagacagtt
     1981 aaaaatacag aacaactaat atttcgcgaa aatttgagcg aagattcaac aggtcggtgg
     2041 ttaagcctgc taattacgcc tgcggtcgcg gtcacggcac cgagtcatgc ttcggaaacc
     2101 atgcgggcca gtgtgttgac gggcgtgggc aacctcgtca tcgaagacca ccccgtgccg
     2161 tcgcccgcac ccacgtgacg ccgaccctgc cgcctcaagt cggtcgtgat tccgaacgct
     2221 tgacattgac tcgaacatag gttcgaatta atcgcatgcg gtgggacggt caaggcgtca
     2281 gcgtcgacga cggcgcactg cccggcctgc agcgcatcgg attcgtccgc agcgtgcgca
     2341 cgccgcaatt cgaaggcatc accttccacg aagttctgtg caagagcgcg ctgaacaagg
     2401 tgcccaacgc gtccatgctg ccgttccggt acaccgtcaa cggctaccgc gggtgttcgc
     2461 atgcctgccg gtactgcttc gcccggccca cccatgagta cctcgacatg aactgcggca
     2521 cggacttcga ctcccaggtc gtggtcaaga ccaacgtcgt cgaggtgctc cggcgtgaac
     2581 tgcaccgcaa gtcgtggcag cgcgagaccg tcgcgctcgg caccaacacg gatccgtatc
     2641 aacgcgcgga aggccgctac gcgctgatgc cgggaatcat cgaggcgctg gcggattccg
     2701 gcacaccgtt ttccattctc accaagggca cgctgctgag gcgtgacctt ccgctgatcg
     2761 ccgacgcggc caagcgggtc gacgtcagtg tcgcggtgtc gctcgc
//
LOCUS       DASYLX010000054         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_441746,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000054 DASYLX010000000
VERSION     DASYLX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441746"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGP27_01365"
     CDS             <1..588
                     /locus_tag="VGP27_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV7849998.1"
                     /translation="ADVVFPVAPVVEKAGSFINWEGRIRPFQPSLPSNATPDLRVLYT
                     LADEIGVDLGLPDAAAAGEELARLGWWGGARPSGPDASAHIAAEPGKGEAVLTGWRML
                     LDSGRLQDGEPYLAGTARPPVARLSASTAAEIGAAQGDPITVATTRGEITLPLEITGM
                     DDRVVWLPLNSPGSAVYQQLGVTAGAVVSIGRVQL"
     gene            585..1805
                     /gene="nuoH"
                     /locus_tag="VGP27_01370"
     CDS             585..1805
                     /gene="nuoH"
                     /locus_tag="VGP27_01370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEV7849999.1"
                     /translation="MTDLSAFGHDPIWLMAIKAVGIFVFLLLTVLAAILIERKLLGRM
                     QMRFGPNRVGPKGMLQSLVDGLKLGLKEGLIPAGVDKPIYLMAPVISTVPAFLAFAVI
                     PMGPVVSIFGHRTPLQLTDLAVAVLYILAVTSIGVYGIVLAGWASGSTYPLLGGLRSS
                     AQVISYEIAMALSFAAVFLYAGTMSTSGIVAAQNGTWFVFLLLPSFLVYLTSMVGETN
                     RAPFDLPEAEGELVGGFHTEYSSLKFAMFMLAEYVNMTTVSALATTMFLGGWHAPWPI
                     NMWDGANTGWWPLLWFTAKVWAFLFGYFWLRATLPRLRYDQFMALGWRILIPVSLVWI
                     AIVATTRSLRNEGHAALATGLVAAGTVAVLVVVVLLWRTVRARTVRRIPVQAVDTGVF
                     PAPPLPPAKEKADA"
     gene            1798..2349
                     /gene="nuoI"
                     /locus_tag="VGP27_01375"
     CDS             1798..2349
                     /gene="nuoI"
                     /locus_tag="VGP27_01375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEV7850000.1"
                     /translation="MPKFLDAIAGFAVTFGTMFKKPITEEYPEKPGPVAKRYHGRHQL
                     NRYPDGLEKCIGCELCAWACPADAIYVEGDDNTEEERFSPGERYGRVYQINYLRCIGC
                     GLCIEACPTRALTMTNEYEMADDNRADLIWGKDKLLAPMQPGMVAPPHPMQPGSTDED
                     YYLGNIKPVPRTQGAEKIPEDVQ"
     gene            2346..3113
                     /locus_tag="VGP27_01380"
     CDS             2346..3113
                     /locus_tag="VGP27_01380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV7850001.1"
                     /translation="MNPEFVALAAASAGHTSTAEAVTFWVFGAVALVGAIGVVAAPKA
                     VYSAIFLATTMMSLAVLYIAQEALFLGVVQVVVYTGAVMMLFLFVLMLIGVDLSESLV
                     ETIRGQRYSALIAGVGFGVLLIAGIGNASASGFTGLSEANSSQNVEGLAALIFTRYLW
                     AFELTGALLITAALGAMVLAHRERFERAKTQRELAEERFRPGGHPTPMPNPGVFARHN
                     AVDLEARLPDGSGSELSVAAILQRRSVDESGAHEERG"
     gene            3110..3409
                     /gene="nuoK"
                     /locus_tag="VGP27_01385"
     CDS             3110..3409
                     /gene="nuoK"
                     /locus_tag="VGP27_01385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV7850002.1"
                     /translation="MNPDNYLYLSALLFTIGAAGVLLRRNAMVMFMCVELMLNAANLA
                     FVAFSRLHGHLDGQVVAFFTMVVAACEVVVGLAIIMTIFRTRRSASVDDASLLKN"
     gene            3417..>4039
                     /locus_tag="VGP27_01390"
     CDS             3417..>4039
                     /locus_tag="VGP27_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV7850003.1"
                     /translation="MTIPIWLLIALPLAGAAILLLSGRRSDPWGHLLGCATSLASFAV
                     GALLFVDMLGRDGEKRAVGEKLFAWVPVGELHVDFGLQLDQLSVCFVLLITGVGSLIH
                     IYSVGYMAEDPGRRRFFAYLNLFLAAMLLLVLADNYLGLYFGWEGVGLASYLLIGFWQ
                     HKPSAATAAKKAFVVNRVGDVGLAIAMMVMFAYIGSISFEGVFAAAP"
BASE COUNT          610 a         1334 c         1327 g          768 t
ORIGIN      
        1 gccgacgtgg tcttcccggt cgcgccggtc gtcgagaagg ccggctcgtt catcaactgg
       61 gagggcagaa ttcggccgtt ccagccgtcg ctgcccagca acgcgacgcc cgatctccgg
      121 gtgctgtaca cgctggccga cgagatcggt gtcgacctcg ggctgcccga tgcggcggcc
      181 gcaggcgagg aactggcccg gctcggctgg tggggcggcg cacgtcccag tgggcccgac
      241 gcgtcggcgc acatcgccgc cgagcccggc aagggtgagg cggtgctgac cggttggcgg
      301 atgctgctcg actccggccg gctgcaggac ggcgagccct atctcgcagg caccgcacgc
      361 ccaccagtgg cgcggctgtc ggcgagcacc gccgccgaaa tcggtgcagc ccagggcgat
      421 ccgatcaccg tcgccacgac gcggggcgag atcaccctgc cactcgagat caccggcatg
      481 gacgaccggg tggtgtggct gccgctgaac tcaccgggat cggcggtata tcaacaactc
      541 ggtgtgaccg cgggcgccgt ggtctcgatc ggacgggtgc aactatgacg gatctctcgg
      601 ctttcgggca cgacccgatc tggctgatgg cgatcaaggc agtcggcatc ttcgtattcc
      661 tgctgctgac cgtgctggcg gcgatcctga tcgaacgcaa gctactcggc cgcatgcaga
      721 tgcgcttcgg ccccaaccga gttggcccca aggggatgct gcagtcgctg gtcgacggct
      781 tgaagctcgg cttgaaggaa gggctgatcc cggcaggcgt cgacaagccc atctatctga
      841 tggcgccggt catctcgacg gtgcccgcct tcttggcctt cgccgtcatc ccgatgggtc
      901 cggtggtttc gatcttcggg caccggactc cgcttcagct gaccgacctt gcggtggcag
      961 tgctctacat cctcgcggtc acctcgatcg gggtctacgg catcgtgctc gcagggtggg
     1021 cgtcgggctc cacctatccc ctgctcggcg ggctgcggtc gtccgcgcag gtcatctcct
     1081 acgagatcgc gatggcgctg tcgttcgcgg cggtgttcct ctacgcggga accatgtcga
     1141 cctccggcat cgtggccgcc cagaacggca cctggttcgt gttcctgctg ctgccgtcgt
     1201 tcctggtgta cctgacgtcg atggtcggcg aaaccaaccg cgcgcctttc gatctgccag
     1261 aagccgaggg cgaactggtc ggcggcttcc acaccgagta ctcgtcgctg aagttcgcga
     1321 tgttcatgct cgccgagtac gtcaacatga ccaccgtttc ggcactggcc accacgatgt
     1381 tcctcggcgg ctggcacgcc ccgtggccga tcaacatgtg ggacggcgcc aacaccggct
     1441 ggtggcccct gctgtggttc accgccaagg tgtgggcgtt cctgttcggc tacttctggc
     1501 ttcgcgccac gctgccgcgg ctgcgctacg accagttcat ggcgctgggc tggaggatac
     1561 tcatcccggt ctcgctggtc tggatcgcga tcgtggccac cacccgcagc ctgcggaacg
     1621 agggccacgc cgcgttggcc acaggcctcg tcgccgcagg gaccgtggcc gtgctcgtcg
     1681 tcgtggtgct gctgtggcga acagtacggg ccagaacggt ccgcagaatc cctgtgcagg
     1741 cggtcgacac gggcgtcttc ccggcaccgc cgctgccccc agcaaaggag aaagccgatg
     1801 cctaaatttc tcgatgcgat agcgggtttc gcggtcacgt tcgggaccat gttcaagaag
     1861 ccgatcaccg aggagtatcc ggagaagccc ggccccgtcg ccaagcgcta tcacggccgc
     1921 caccagctga accgttaccc cgacggcttg gagaagtgca tcggctgcga actgtgcgcg
     1981 tgggcctgcc ccgccgacgc gatttacgtc gagggtgacg acaacaccga agaggaacgt
     2041 ttctcgccgg gcgagcgcta cggccgggtg taccagatca actatctgcg ctgcatcggc
     2101 tgcggcctgt gcatcgaggc ctgcccgacg cgcgcattga cgatgaccaa cgaatacgag
     2161 atggccgacg acaatcgcgc agacctgatc tggggcaagg acaaactgct cgccccgatg
     2221 cagccgggca tggtggcgcc accgcatccg atgcagccgg gcagcaccga cgaggactac
     2281 tacctgggca acatcaaacc tgttccgcgc acgcaaggag cagaaaagat tcccgaggat
     2341 gtgcagtgaa cccagaattc gttgcgctcg ccgccgcttc cgccggccac acctcgacgg
     2401 ccgaggcggt cacgttctgg gtgttcggcg cggtcgccct ggtcggcgcg atcggcgtgg
     2461 tggccgcgcc caaggccgtg tactcggcca tcttcctcgc gacgacgatg atgtcgctcg
     2521 cggtgctcta catcgcccag gaggcgctgt tcctcggcgt ggtgcaggtg gtcgtctaca
     2581 ccggcgcggt gatgatgctg ttcctcttcg ttctgatgct gatcggtgtc gacctgtcgg
     2641 aatcgctggt ggaaaccatt cgggggcaac gctattcggc gctgatcgcg ggcgtgggct
     2701 tcggggtgct tctgatcgcg ggcatcggca acgcgtcggc gtccggcttc accggcctgt
     2761 cggaggcgaa cagcagccag aacgtcgaag gcttggccgc gctgatcttc acccgttatc
     2821 tgtgggcgtt cgagttgacc ggcgcgctgc tgatcaccgc tgcgctcggc gcgatggtgc
     2881 tggcgcaccg tgagcgcttc gagcgtgcca agacccagcg cgagctcgcc gaagagcggt
     2941 tccgccccgg cggccacccc accccgatgc cgaatcccgg cgtattcgcc agacataacg
     3001 ccgtggacct cgaggcacga ctgcccgacg gctcgggctc cgagctgtcg gtcgccgcga
     3061 ttctgcaacg acggagcgtc gatgaatccg gcgcacacga ggagcgtgga tgaatcccga
     3121 taactaccta tatctgtccg cgctgttgtt caccatcggg gcggcgggag ttctgctgcg
     3181 ccgcaacgcc atggtcatgt tcatgtgcgt ggagttgatg ttgaacgcgg ctaacctcgc
     3241 cttcgtggcg ttctcccgct tgcacgggca cctcgacggc caggtggttg cgttcttcac
     3301 catggtggtc gcggcctgcg aggttgtcgt cggccttgcc atcatcatga ccatcttccg
     3361 cacccgccgc tcggcctcgg tcgacgacgc cagcctgctg aaaaactaag gccgcgatga
     3421 cgattccgat ttggcttctg atcgccctgc ctctggcagg tgcggcaata ctgctgctgt
     3481 cgggtagacg atccgatccg tggggccacc tcctcggctg tgcgacgtct ctggcgtcct
     3541 tcgccgtcgg tgcgctgctg ttcgtcgaca tgctgggcag ggacggcgag aaacgggccg
     3601 tcggtgagaa gctgttcgcg tgggtgcccg tcggcgaact gcacgtcgac ttcggcctgc
     3661 agctggatca gctctccgtg tgcttcgtgc ttctgatcac cggtgtcggc tcgctgatcc
     3721 acatctactc ggtcggctac atggccgaag accccggccg ccgcaggttt ttcgcgtatc
     3781 tgaacctgtt cctcgccgcg atgctcctgt tggtgctggc cgacaactac ctgggcctct
     3841 acttcggctg ggaaggcgtc ggcctggcgt cctacctgct gatcgggttt tggcagcaca
     3901 agccgtcggc cgccaccgcg gccaagaagg cgttcgtggt caaccgcgtc ggcgacgtcg
     3961 gcctcgccat cgcgatgatg gtgatgttcg cctacatcgg ttcgatctcg ttcgagggcg
     4021 tcttcgccgc cgcaccgca
//
LOCUS       DASYLX010000055         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3513639,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000055 DASYLX010000000
VERSION     DASYLX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513639"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..338)
                     /locus_tag="VGP27_01395"
     CDS             complement(39..338)
                     /locus_tag="VGP27_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850004.1"
                     /translation="MKKIGLATIVAGGLVAATLGLAAPAVAAPSGPGNAQETINDLKA
                     DGYNVIVNRVGGTPLEQASVLAVRPGQTYSRTDSGNPGDSLETTVTNKTVYVDVK"
     gene            complement(601..2346)
                     /locus_tag="VGP27_01400"
     CDS             complement(601..2346)
                     /locus_tag="VGP27_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7850005.1"
                     /translation="MTQGVGLSVGAANVAAVIPGCAAVTRQPVLTLYRHRPPEVGVPA
                     EYPWPAQRGLVVNDFVDRAADPVGIVAPDGSVHRGEALIADALRAVIRAVTGGRAPQS
                     PPGVAFPAHWRPPAVDALRRELLRRPEWPRPDRLPLMSDATAALTALQADPGLPTRGV
                     IALCDFGGTGASITLIDAAGGYQAIGATARHAEFSGELIDRALLAHVIADLSISGTSD
                     VAGTAAFGSLHRLRAECRGAKERLSRGAVTTLFADVPGFRGDVRLTRAELDGEIRQPL
                     AAFIDALREHLFRNRIHPADLSAVASIGGGASIPAVTTALSDQLRVPVISTPHPELTA
                     AHGAALLAVRRPEDEIRTAVESPVQSTAMRPLAWSELDNVPEIAPFAIESEAPAGLSA
                     ARPRVDFVPEDKPARHKPSAVPWYRPLLVTAGVFAAMVLVGTGAVLAIRNDASARTGT
                     TTTTTTVQPDPAAPAAAPPTALAVTPEPTPNAPSYIRPPAPVTRVVVTTPPPVVMQAP
                     APPPSAPPTSTPTTTSTPNTTSPPPSSPPSSPPSSAPSEPPSSPPSSAPPSSEPSTPP
                     SPDKPPGEQTDQPDV"
     gene            complement(2392..2799)
                     /locus_tag="VGP27_01405"
     CDS             complement(2392..2799)
                     /locus_tag="VGP27_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV7850006.1"
                     /translation="MADFDARERFASSPVAQLATAGADGVPHLVPVVFAVSNDIIYTA
                     VDAKRKSTHRLRRMANIEANPAVSLIVDHYDHDWSQLWWVRADGIAEIHYSGEEMATA
                     YFELRRKYVQYQRIALDGPVITVTVARWSGWQA"
     gene            2884..3495
                     /locus_tag="VGP27_01410"
     CDS             2884..3495
                     /locus_tag="VGP27_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850007.1"
                     /translation="MAGSRTVHVQPRNRPGRPRSEESRAAVLRATSELLNEVGLRAMT
                     TEEIATRSGASKATIYKWWPNKYAVAIDAFLSEMMAESRDPDTGSAHDDLRAVMRGLT
                     HFYTGQSGRVYAQLVGEAQTDPLVQQELRANLVESRRDLFRTIWDRGVARGELRTDID
                     REVAIDLVVGPALYRLMMGHAPLTAATADAIVDAAIRGLTAGD"
     gene            3583..3825
                     /locus_tag="VGP27_01415"
     CDS             3583..3825
                     /locus_tag="VGP27_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850008.1"
                     /translation="MKLRYMSALLAAGAASAAIVAAPTAAAAPTGPLCTYVGAQTTQC
                     QSPGNVQINSDHQVTYPDQYPFFGNVLLFHHGGHHR"
     gene            complement(3837..4409)
                     /locus_tag="VGP27_01420"
     CDS             complement(3837..4409)
                     /locus_tag="VGP27_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850009.1"
                     /translation="MPEKGASGDFDYLREVTDGSDGVDVVAEENAYRSEADLDYLLGE
                     DVSRNRDGFDAFDENTWDEDFTAVSTTPWYRSPQARTLMIASGAALSAIVVSAVLLLF
                     RGPPAADHPAPADSSTPVTTPLATATSEMPPPPPPPPPPPEPSVVNDAPAVVRPTVRP
                     RPTKQPEIGVTRTPVTRQPISVAPQPRRPR"
BASE COUNT          747 a         1500 c         1593 g          794 t
ORIGIN      
        1 caccccgatg gggtgcccct cgctccgtcg caagtgtgtt acttgacgtc gacgtacacc
       61 gtcttgttgg tgacggtggt ctcgagcgaa tctcccgggt tgccggagtc cgtccgcgaa
      121 taggtttggc cgggtcgcac ggccaggacg ctcgcctgct cgagcggggt tccgccgaca
      181 cggttcacga tgacgttgta gccgtcggcc ttgaggtcgt tgatggtttc ctgggcgttg
      241 cccgggccgc tcggtgctgc gacggcaggg gcggccaatc ccagagtcgc tgcgaccagt
      301 ccgcccgcga cgatggtagc taatccaatc ttcttcatct ttggtgcctt cttcttttct
      361 gttgtgtgcg atgcagaccg tcggcctaac cgcgattgat gccggacgac atgggattcg
      421 ttccgactga tctgataaac cgacaggtca gcgctttaat tccgatggca gtaaatgatc
      481 gccggttggc aggatctcaa tgttcgaata gcccaaaacg cgaatttcgg aacggctgtc
      541 gtctaccggg tcgcggtcga caacaacgaa cagcaaaggc ttattcccgg ccctccgcgc
      601 ctacacatcg ggctgatcgg tctgctcgcc cggcggcttg tcgggtgacg gcggcgtcga
      661 cggctccgaa ctcggcggcg ctgacgacgg cgggctgctc ggcggttccg aaggtgctga
      721 tgacggtgga ctgctcggcg ggctactggg tggcggggac gtggtgttcg gtgtcgacgt
      781 ggtggtcggg gtcgacgtcg gcggcgcgct cggcggcggc gcgggtgctt gcatgaccac
      841 cggcggcggc gtcgtcacca cgacccgggt caccggtgca ggcggacgaa tgtacgacgg
      901 ggcgttcggc gtcggttccg gtgtcaccgc gagggcggtt ggtggggccg ccgcgggagc
      961 cgccggatcg ggctggacgg ttgtcgtcgt cgttgtcgtc cccgtcctcg cggaggcgtc
     1021 gttgcggatg gccaggacgg cgcccgtgcc gacgagcacc atggcggcga atacgccggc
     1081 ggtgacgagg agcgggcgat accacgggac ggccgaaggc ttgtggcggg caggcttgtc
     1141 ctcgggtacg aagtcgaccc ggggacgggc cgcgctcaga ccggccggtg cctcggattc
     1201 gattgcgaat ggcgcgatct cggggacatt gtcgagctcc gaccaggcca gcgggcgcat
     1261 tgccgtcgac tgcaccggtg actcgacggc ggtccgaatc tcatcctccg gtctgcggac
     1321 ggccagcaga gcggcaccat gcgcggcggt cagttcggga tggggcgtgc tgatcacagg
     1381 cacccgaagc tggtcggaca gcgctgtggt gaccgccggg atactcgcgc cgccaccgat
     1441 ggatgccacc gccgacagat cggcgggatg aatccggttg cggaacaagt gttcccgcaa
     1501 cgcgtcgatg aatgccgcaa gcggctgccg gatctcgccg tcgagttcag cgcgggtaag
     1561 ccgcacgtcg ccgcggaacc ccgggacgtc ggcgaacagg gtggtgaccg cgccgcggga
     1621 cagacgctcc ttggcgcctc ggcattcggc gcgcagccga tgcagcgatc cgaatgcggc
     1681 cgttcccgcc acgtcggagg tgccgctgat ggacagatcc gcgatcacgt gcgcgagcag
     1741 tgcacggtcg atgagttcgc cggagaactc ggcgtgacgc gccgtcgcac cgatggcctg
     1801 gtaccccccc gccgcatcga tcagcgtgat gctggcgccg gtgccgccga aatcgcagag
     1861 ggcgatcact ccacgggtcg gcagtcctgg gtcggcctgc aatgcggtga gcgccgccgt
     1921 ggcatccgac atcaacggca accggtccgg ccgcggccat tcgggccgcc ggagcagttc
     1981 cctgcgcagg gcgtcgacgg ccggggggcg ccagtgggcg gggaaggcga ctcccggcgg
     2041 tgactgcggt gcccggccgc cggtgacggc gcggatcacc gcacgcagcg cgtcggcgat
     2101 cagtgcttca ccgcgatgga cggagccgtc gggggccacg atgccgaccg gatcggcggc
     2161 ccgatcgacg aagtcgttga caaccagccc ccgctgcgcc ggccaagggt actcggcggg
     2221 cacgccgacc tccggcggac gatgccggta gagggtgagc actggctgtc ttgtgacggc
     2281 cgcgcaaccc ggtatcacgg cggcgacgtt cgcggcgcca accgacagcc ctacaccctg
     2341 tgtcatgtca cgacgtttat cgtcgtagcg agcccaggta ttaccggcgg atcaggcctg
     2401 ccatcccgac caccgggcca cggtgacggt gatgacgggc ccgtcaagag cgatgcgctg
     2461 gtactggacg tactttcgcc ggagctcgaa gtatgcggtc gccatttctt caccgctgta
     2521 atggatttcg gcgatgccgt ctgctcgtac ccaccatagc tgcgaccagt cgtggtcgta
     2581 gtggtcgacg atgaggctga ccgcggggtt ggcttcgata ttggccatcc tgcgcagccg
     2641 gtgagtggat ttccgtttgg cgtccaccgc ggtgtagatg atgtcgttgc tgaccgcgaa
     2701 gacgacaggg accagatgag ggacgccgtc ggcacccgcc gtcgcaagct gtgcgacggg
     2761 ggaggaggcg aacctctccc tggcgtcgaa gtcggccatc ttctcctcag gttgcgatag
     2821 cgcccgaatg gtcaatagta gggaatatga acgctacgtt cattatctgt ggagaggtga
     2881 ggaatggcgg gtagccgcac agtgcacgtg caaccgcgca accgccccgg ccggccgcgc
     2941 agcgaggagt cccgcgccgc ggtactgcgt gccacctctg aactgttgaa tgaggtcggg
     3001 ctgcgcgcga tgaccaccga ggagatcgcc actcgcagcg gtgcgagcaa ggcgaccatc
     3061 tacaagtggt ggcccaacaa gtacgcggtc gccatcgacg cgttcctctc cgagatgatg
     3121 gccgagtcgc gcgatccgga cacgggttcg gcgcatgacg acctgagggc cgtcatgcgt
     3181 ggcctcacgc acttctacac gggtcagtcc gggcgcgtgt acgcacaact cgtcggcgag
     3241 gcacagaccg acccgctggt ccagcaggag cttcgcgcga acctggtcga atctcgccgc
     3301 gacctgtttc ggacgatctg ggatcgaggt gtcgcgcgcg gcgaactgcg gaccgacatc
     3361 gaccgcgaag tggcgatcga ccttgtcgtc gggcccgcac tgtatcgcct gatgatgggc
     3421 cacgcgccgc tgaccgccgc gacggccgac gcgatagtgg atgccgccat ccgcgggctc
     3481 acggcgggtg actgacatca catgaggcgc tggtatgacc ctgcccgcgc cgacgttgaa
     3541 actaaacgtt cagtttagta atcaccgatg tctggagctt ccatgaaact gcgttacatg
     3601 tccgcattgt tggcagcggg cgccgcgtcg gccgccatcg tcgccgcgcc gacggcagcc
     3661 gccgcaccga cgggcccgct ttgcacttac gtcggtgcgc agaccaccca gtgccagtca
     3721 ccgggcaatg tgcagatcaa cagcgatcac caggtcacct atcccgacca gtacccgttc
     3781 ttcggcaacg tcctgctgtt ccaccacggc gggcaccacc gctaaagggc gatcgattac
     3841 ctgggtcggc gtggctgcgg ggccacactg atcggctgcc gcgtcaccgg cgtccgtgtc
     3901 acgccgatct cgggttgctt cgtcggcctc ggccgcaccg tcggccgcac gacggcaggg
     3961 gcgtcgttca ccacggacgg ttccggcggc gggggcggcg gcggtggcgg aggcggcatt
     4021 tcgctcgtcg ccgtggccaa aggtgtggtg accggtgtcg agctatccgc aggcgccgga
     4081 tgatcggctg cgggaggacc ccggaacaac aacagaaccg ccgataccac gatcgcggag
     4141 agcgccgcgc cgctcgcgat catcagggtg cgcgcctgcg gcgaccggta ccagggagtc
     4201 gtggagaccg cggtgaaatc ctcgtcccac gtgttctcgt cgaatgcgtc gaacccatct
     4261 cgattgcggg acacgtcctc gcccaacagg taatcgaggt ccgcctcgga cctgtatgca
     4321 ttttcctcgg cgacgacgtc gaccccatcg ctcccgtcgg tcacttcccg caggtagtcg
     4381 aagtcccccg aggccccctt ctccggcacc ctctgatgct aacgccacca cctgggaaaa
     4441 cgcgcgctca cggtagatga atatcgactc acagcaaccg cctgccgccg actcgcggcc
     4501 acgatagggt tccaacgccg ggtgatccat tcatcgcgat gaccctgcgc cgtcatcgcc
     4561 gcgccagcac tggtttgcct atccatcgga aggttcgccc atgaagaaat cgcgcctctg
     4621 cggcgtcacc gcac
//
LOCUS       DASYLX010000056        26939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3184465,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000056 DASYLX010000000
VERSION     DASYLX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26939
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184465"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGP27_01425"
     CDS             <1..443
                     /locus_tag="VGP27_01425"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEV7850010.1"
                     /translation="GYHLDLGAKLAPLRERGVLVIASGNVVHNLRAMDWSLGDSGYDW
                     AKRFDEHTKALMLSNPVDAAGLDEHTDFRTAVPTPDHFIPLLYLAGLAGVATDKLDIL
                     VDGYAYGSMSMTAYTLGLACADIGGSQVDTLQPASDFPADASNI"
     gene            1486..1617
                     /locus_tag="VGP27_01430"
     CDS             1486..1617
                     /locus_tag="VGP27_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850011.1"
                     /translation="MANRGNALVRRPAVVFGLVIAHEPLAGDAHDLAATANVPAASL"
     gene            complement(1722..3962)
                     /locus_tag="VGP27_01435"
     CDS             complement(1722..3962)
                     /locus_tag="VGP27_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV7850012.1"
                     /translation="MTATMTGPPRPPEPPQPSAAEVAAPKRNLIFTAVLLGMLLAALD
                     QTIVATALPTVVADLGGAGHQAWVVTSYLLASTVVTAIVGKLGDLIGRKTVFIAAVLF
                     FMAGSVLCGLAGSLPMLVAARALQGVGGGAIMVTATAVIGEVIPLRERGRYQGALGAV
                     FGVTTVIGPLLGGFFTDNLSWRWAFWINVPVAVVVIVVAVAAIPNLGRMARPVIDYAG
                     IVLVGLGATGLTLATSWGGNTYAWGSPMIIGLFVGSLVALGAFVAVELRAAEPILPMR
                     LFREPVFAVCCALSFVVGFAMLGALTFLPTFMQFVDGVSATTSGLHTLPMVLGLLTMS
                     MSSGVIVGRTGRYKIFPVAGTAVMAIGFVLLSRMGPDTSTVLQSVYLVVLGAGIGMCM
                     QVLVLVVQNTVDFSDLGVATSGVTFFRAIGSSFGAAVFGSLFNNFLHSRLAAALASSG
                     APPEAAQSPDALHKLPPEMAAPIAAAYADALDLVFLYAAPVAVVGFVLALMLKQVPLR
                     NNDVGTAADMGEGFAMPTSASPDQVVENAVGRLIRHGSGMQLRAIAERPGSKLDIGRL
                     WALLQVFRFGQAAGQARLGDIAEHVRVPREILEPAFDRLAATGYAERSGDVFWLTSAG
                     AHQVELVRAETMDWLTQRLSQSSGLQVRPDRGQVREALDRITQDVLVQRDWTDDETHA
                     MQMRPPRRALHPKRPGPAPRPPAPVRPRPPVPDPRGRAPRPTEPPTTRLRPPGPPPRG
                     PRSPQR"
     gene            complement(4601..5155)
                     /locus_tag="VGP27_01440"
     CDS             complement(4601..5155)
                     /locus_tag="VGP27_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850013.1"
                     /translation="MTATSQDVLSDAQIGALSAEQRRELITRLERPVAEVRSHGWMAP
                     LRQAHLGLMTGGAVFMIPWIVYLGFTLPQNYTVRDWPVTWLGFDSLLVVFMIATAVLT
                     WLHRQVLVFPAFTTGVLLICDAWFDLTTANPDELLTSLLTAGLGTLLAAVLIGGSLSL
                     VRYNASRLWMLQPGQSLWRLPLLT"
     gene            complement(5213..6631)
                     /locus_tag="VGP27_01445"
     CDS             complement(5213..6631)
                     /locus_tag="VGP27_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEV7850014.1"
                     /translation="MTARAATTYNGARAVLARTLDSQRTLVATSSRGAHRLGAVALAG
                     GFLVVGYMAARDHDAADWQPSGRLAWSLSLLVASMLLSRGVYLGRPVTGRHAAAALAM
                     VLAGIGAHMLSAGIIGDVLVAASGLALMWPLPSPPGPGAEWVWPLICSTRGDALAPFA
                     MASSKSVFFTDDHTAALSYRARLGFAVVSGDPVGEPSHYRALVRDFAAMCATRGWRIV
                     VLNCGERCVGLWRDASVVHQPLMSVPVGRDVVVDVAQFALSGRKLRNLRQAVARTRKR
                     GITTEVIPEQSLSAALREELLDVVSTSRRGGRYERGFSMMLDGVLTGRYPGVLVIVAR
                     DAAGIVQGFHRYVVSGGGSDASLDLPWRRPGAPNGVDERLTVDMIDWCREQGGRRLSL
                     AFAAFPELFDETNRTGLQRIYYSLIRLGDPLIRLESLYRYLRKFHALGARRYVLIPAV
                     QLLGALVVLLSLEFVPHRSRAC"
     gene            7296..7718
                     /locus_tag="VGP27_01450"
     CDS             7296..7718
                     /locus_tag="VGP27_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850015.1"
                     /translation="MELARSVHLRLAAGVAALAALWIWAAAGGGVADAATSAPDVTGK
                     KYSDAQSAISGAGFTPVVSTVFGDRNTWSNCLVTETKQRTVQPPPNSGGSAVKQVLVS
                     LNCDAGVASATRPGNSAASPEGRAATKAAAASPTGSSS"
     gene            7818..8552
                     /locus_tag="VGP27_01455"
     CDS             7818..8552
                     /locus_tag="VGP27_01455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV7850016.1"
                     /translation="MHIDRRDLLKAAATGVVAATAGAPTALADAGRSALPRARVPAPT
                     GVVTRLPGDGNQLALTIDDGTSTDVVAAFAQFCRDSGTRLTFFVNGANASWRHNAEAL
                     RPMVNSGQIQLGNHTWSHPDITRLRASDLTDQISRNKDFLVNTYGVDGTPYFRPPYGR
                     HNSQTDHVPADLGYPTIPLWSGTIGDSRPINETNLIAAATQSFTPQAIVLGHANLPTI
                     THTYSQLLDIIQGRNLQTVTLADVFS"
     gene            8916..12092
                     /locus_tag="VGP27_01460"
     CDS             8916..12092
                     /locus_tag="VGP27_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850017.1"
                     /translation="MTARRLVCGSCGTELLLNSKFCNECGAPVTQVNRSAEYKQVTVL
                     FADVVHSMDIAAAVGPERLREIMAELVDCAGAVVRRYSGTVDKFTGDGIMAIFGAPVS
                     LEDHAVRACLAALGVQKEAKRLASDVQDRDGVELQLRMGLNSGQVIAGEIGSRPIGYT
                     AVGEQVGMAQRMESIAAPGGVMLSASTARLVDGTAALGEPERVRIKGTNEPVTAYRLL
                     GIGDRHRATERGEANLVGRRWEISAVERLLDRALAGHGAVVGVVGEPGIGKSRLVREV
                     AATAAVRGGKVFSAFCESHTSHVPFHAVGRLLRAATGVEGLDGQAARARVRDQFPDAD
                     LEDALLLDDLLGIADLNTAIPAIDPDARRRRLAALVNAARLSRQSPAIYVIEDAHWID
                     EVSESMLTDFLAVIPQTPSLVLVTYRPEYHGALARVAGAQTVTLAPLSDSETAALVAH
                     LLGADPSVDALGQRVAERAAGNPFFAEELVRDLAERGVLRGEPGAYISAAEVGAVRVP
                     ATLQATIAARIDRLHPKAKRTLSAAAVIGSRFGLGLLTVLGVEPVVDELVAAQLVDQV
                     SSTRQPEYVFHHPLIHSVAYEAQLKSDRSELHRRVATAVEAREPESGDENAALIAKHL
                     EAAGDLQAGYGWHMRAATWATNRDISAARLSWERAAEIADALPAEHPHRAAMRIAPRT
                     MLCGTGWRVHANVAGDRFDELRELCSAAGDKASLGIATAGLVVGCAHQDRVREASHLA
                     SEAWALAEAVGDATLTVGLSFPVIYGQIESAEWSDVLRWSQTVIDLADGDLSKGNFLV
                     GSPLALAFASRGMARYFLGRPGWPEDLRHSLAMARRADPASYAGVVGYVYLLGVPFGV
                     LRPDDPALQAIEDALRIAERSSDDVVVGFTKATLALALVHRQNAAERDRGQKLGAEVS
                     QVFLNQGHNLCDLPIVEVYLARERARGGEIDGAIPLMRAACDHLFRDGRLLLWGVPAT
                     GVLVETLLDRCADGDVTEAEAAIERLAAAPADEGLVMRDIWLLRLRALLARAHRDDAA
                     YARFRDRYRGMVESLGYEGCIAWVDAMA"
     gene            complement(12964..13284)
                     /locus_tag="VGP27_01465"
     CDS             complement(12964..13284)
                     /locus_tag="VGP27_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850018.1"
                     /translation="MTAQSAIPSALEARSLYRFYRAGDAEPVALQAVSLRLERGEFVA
                     VTGTSGPGKSTLLACLAGMDKPDGGSVHIAGRRISHRPEPQHAPIPARHIGMLFQNSN
                     QLAG"
     gene            complement(13281..13661)
                     /locus_tag="VGP27_01470"
     CDS             complement(13281..13661)
                     /locus_tag="VGP27_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850019.1"
                     /translation="MGPQHRRAATIFTDKSNTVRIETRPLATAPDTESVNIDEVPAIA
                     SSTPGYRLGMVTPVQRKAGPAMVIIYQDTSPRNPVTGKTGTDAVERYEFWRDGHEVIL
                     TLSGAVGADNVDAWRTVTDSLQWR"
     gene            14252..14434
                     /locus_tag="VGP27_01475"
                     /pseudo
     CDS             14252..14434
                     /locus_tag="VGP27_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246198.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            15126..15305
                     /locus_tag="VGP27_01480"
     CDS             15126..15305
                     /locus_tag="VGP27_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850020.1"
                     /translation="MIYVNDNYGDFAATREDIVRQALDGADPELVKSILPDQNWAFVD
                     ILLYLHGHHICVIPG"
     gene            complement(15302..16354)
                     /locus_tag="VGP27_01485"
     CDS             complement(15302..16354)
                     /locus_tag="VGP27_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7850021.1"
                     /translation="MTSTADGVDLVPWVDERLRKVAQLIRRSMLDAMPDGEPSRWLYA
                     PMREYPSRAGKALRPALCLSAGRAFGGDTEDLLGVAVAIELLHNAFLVHDDIADGSEM
                     RRGRPTLAASYGLSAALNAGDGLAVVAGQVLRQATRKFDRDLADRVWTEFDTMALRTL
                     EGQALEVGWQFDNVEDLTPSDYLELIMHKTCWYTTIHPLRVGALVGSRGTAELDLLIR
                     FGFHFGAAFQIRDDLLNLVGDESVYGKEILGDLYEGKRTLPLVHLSANARAADAEIVR
                     DYLRRSRDDRTADQVQTIRDMMDRYGSIQYAAEFAEGILSVAEDHFEAAFAGAEPGPD
                     LDFLRSLVPYVWARWR"
     gene            complement(16351..17034)
                     /locus_tag="VGP27_01490"
     CDS             complement(16351..17034)
                     /locus_tag="VGP27_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850022.1"
                     /translation="MNRGIDDAGFSDLPWVSVFDPAANARALNSIQAEGFRAASELVD
                     RFVRIARGGLNGTDWPTISSAPLNNEQRADIFGATDVAPLLKSWWSVIGQMLLGAEAQ
                     VSSGPEEPVVDLEKVETAGRIELVTTAPGVATTEVWLHNRGTADFGDVTVHCSELLAH
                     DGNIIPSDSVRVMPQIVAMPPRSSRGIAVEVSVAVDVAPGTYRGNLLASGGSGLWLAV
                     ILAVEAAPS"
     gene            17117..18604
                     /locus_tag="VGP27_01495"
     CDS             17117..18604
                     /locus_tag="VGP27_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850023.1"
                     /translation="MGMLRDLIEVYGALDKAVGRSNDPAVGNAVDSFVPGFSANCGWY
                     DEIERFFFGKNAKSNPDLGEACSRIGAASKAADSQRAASASAGDGEGGGRLPRPALTK
                     PQKSRLLFGLTAAARAHQAAAPKMTYMPDDYLMYAYMEALAALGPDDEPYDSRLGKAG
                     AKFEDIWNEVDARCAGRGQFSRLRKYAATTAGVVDPSTQDIPLCSAKVVTIDGLPVVV
                     VDTNLHSDSRSFHDVVEIVNPFNWNENYPEFFLSMDPCDPPERTDGWFRVLETVGFPG
                     LGGLELTTNLKYYPKLKLDEGIAHIDYDLDDPPPGQGDGKVIVDRGFINIEVANDERD
                     PTKPGVSAKTRKVVHIEGLSPYAQQRLVCLTGYGTASSEFLLGGKADAEKPYPRDFVY
                     YKDKVPQADPEAEPTEVSTHAVATAVNLWTESVQGLVSDYFDFAQKWTDGRLRIRDVT
                     DYSTQVTERLVTGPLEFWDRVNQPRRTGGQSGHARPRGGRGGNPE"
     gene            18631..19125
                     /locus_tag="VGP27_01500"
     CDS             18631..19125
                     /locus_tag="VGP27_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850024.1"
                     /translation="MRTRQAKQMLRGYMNEVNTITENASVDDETGRWGLDPWIRIIHE
                     LVDLQLRTTASIIQGALAGPSWLQQTSEELDAETVDVSPSAPYPRIVSASAFVRVGQP
                     TTKIPPASLDLEPDVLPAGATQIQIRLKDYRYVGANYKGKITLKNANDPSATPQTVEI
                     TVGL"
     gene            19122..22310
                     /locus_tag="VGP27_01505"
     CDS             19122..22310
                     /locus_tag="VGP27_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850025.1"
                     /translation="MTDRSTKSTIDKLLDRAISAITRGDRATADALAGQVLAVDRNNP
                     DAEELLANPTGGEIRRLTMLFADLVDSTALSTRIDPEIYRTVVGRYRDLVTAIVNRFE
                     GHIGSTRGDGLLAVFGHPIAHENDAHRAVQAGLDIINEVAVLSWRVERRFGFDIDVRV
                     GVHRGVVYLDIGQDDVYGLGANLAARVCSLAEPGSVAVSEAIEHVVHDRFELEERPPA
                     PIKGVDEDIVHYRVIADRDVTAIARGPLVGREGELEYLSDSWRKAVAGSLSDPCIAFC
                     GEGGIGKSRLAGAAVEMAKQSGAVVLDLYGSPFHTDIGLRPVRRLLERRCGIHRGSDP
                     VESLGKLATEVEGYGISADVAVPLLAPVLGIGPESGYRPTPARAGKLYDQIAGAIKEY
                     LLACIGTGPGLVLVEDIHWFDEDTIEIVQALLQDKSGRLLVVVTGRQLPAAIDCAQTY
                     SLGPLTDAEADTLIQALHFELTPAARASVRQRCDGIPLYIEEVVAKLKNLSEATGESA
                     QVPDTLYEVLMARLQSSRHAPLVVEAAALVGRHIDHRLLASAVQLGTEKVDALLDEFT
                     RGGVLQRIGPDSWRFRHELLREVAAELSPPSVRRRLHSRIGDALVGAADDTAPEWALV
                     AHHYERAERFDEAVVACQNASANARQRGALIEARNHLTRALENVDLMPAGPTRDRNEV
                     AVRLERGFLTSVATGHASTEAAAEFERCLQLVGDEPSSQLYATFTGLWSYYATRGDLH
                     RATQLVEALRNLPDMPEWYEVANDVVLGVLATFRGEFRPARAMLESGATALDRIGSPQ
                     IEGAWFAPNDPLAGAHTFLGLLRFFEGDLAGAESAFALVEGHCKTLAFPHGPFTLCYG
                     LAMETMARVEAGQLEEANQLVAEVAGRAEQHGFDEWAMISVCNQASLAARAALAAGET
                     HAATLHAHIAAITAVVDAWRGADMKTFLGVYDGLLARLLIAVGMKSEARERAELALQM
                     ADETGIHFYDAELLRIRAHTHDDADARHAELRGAIVLAQQQGHPVFELRAAADVFELV
                     GESSRTVLEDALSHFPVDQTWPELARARALLR"
     gene            22307..24361
                     /locus_tag="VGP27_01510"
     CDS             22307..24361
                     /locus_tag="VGP27_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7850026.1"
                     /translation="MSTPRGRVAILGGGMAGLSAAWRLSEPGWRDRFESITIYQRGWR
                     LGGKAASSRGPHGRIEEHGLHVWLGSYENAFALLRECYAELDRPTTAPQTPIRTWDQA
                     LVPADNLAMADQSGRDWSVWPGRFSRNDQLPGDPEATGRELTVVEFLRRALQLVLDFA
                     ESLDETAGLVLSATSDPKATEWREVTRRAAFATIAALSASPGRRVLHAGLLAQSLEAI
                     RGALDYERRPDHKRFWLMLALLVATARGLITDNLVTDPRGFRAINDEDYGHWILRHGA
                     PVEVLDFPLVRGMYDMVFGYEDGDYTRPSFAAGLAVLLTGIALFQYKGAFFWKMTAGM
                     GDVVIAPLYQALRKRGVRFEFFHRVDQLHLDERRLVIDAITVGRQVAMAEGIENYEPL
                     TTVKGLPVFPKAPLADQIVAKAGIEDLESHFARRDDVETSVLRRGVDFDHVVLATSLG
                     MVEMVAAELIADRPEWLEMTTHLRTVATQAFQLWLRPLESQLGWHLPGVTTSGYVAPF
                     DTWASMPQTLWAENWPDEDRPRTIAYFCGAFNAQWPPDGDPADYVASCRQQVLTNAVT
                     YLDHHLSLYLPGAVTGNGFVWNLLSGVNGDRGAAALDRQHVSVNIDPSDRYVQSVPGS
                     DKYRLRSDESGYDNLVLAGDWTDSGINAGCIEAAVMSGLQAANALLGRSRFHRIRGFY
                     LP"
     gene            complement(24370..25620)
                     /locus_tag="VGP27_01515"
     CDS             complement(24370..25620)
                     /locus_tag="VGP27_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV7850027.1"
                     /translation="MGSSRRDLDIVVYGATGFIGKLTANYLANSRAEARVALAGRSLD
                     RLHAVRSSLGTAAQEWRLIVADVSQPEALRAMAARTQVVVNAVGPYTTHGLPVVAACA
                     ATGTDYADLTGEVPFVRNSIDLYHSQAIESGSRIVHSCGFDSVPSDLTAYALHRRAID
                     DKAGELTDTTLVLRDYSGGYAGGSIATMVELMRIASDDAGVRRMLDDPYSLSPDRRAE
                     PDLGDQPDLPLCRGAQISPELTGLWAGGYVMALYNTRCVRRSNALLGWTYGRRFRYAE
                     TITMGSTVLSPFIAAMFNAGIANASRYGGEFLRMLPRGFADRMMPRAGIGHDEGTRGH
                     YKVETYTTTTSGARYVATMAQQADPGYTATSVLAGECALALALDRDKLSDTRGVVTPA
                     VAMGDVLMTRLPAAGVTVKTAQLS"
     gene            complement(25632..25765)
                     /locus_tag="VGP27_01520"
                     /pseudo
     CDS             complement(25632..25765)
                     /locus_tag="VGP27_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925936.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(26206..26592)
                     /locus_tag="VGP27_01525"
     CDS             complement(26206..26592)
                     /locus_tag="VGP27_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7850028.1"
                     /translation="MYLRSDVLGEVDTSPESAEPGMWAVDGLPSDVAVLLMKRGPNAG
                     SRFLLDRAATSVGRHPNSDIFLDDVTASRRHAEFRRENGQFLVVDIGSLNGTFVNRKP
                     VQSSVLANGDEIQIGKFRLVFIDQDG"
     gene            complement(26708..>26939)
                     /locus_tag="VGP27_01530"
     CDS             complement(26708..>26939)
                     /locus_tag="VGP27_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850029.1"
                     /translation="ARCAMWFMSLGKVDKNAPTPTGEQHQLLAPDTITAQTPYGELVR
                     LAPPVEFSETKPRWKNPLLVVRGSSKPQWTTG"
BASE COUNT         5077 a         8694 c         8548 g         4620 t
ORIGIN      
        1 tgggctacca cctcgacttg ggcgccaagc tggctccatt gcgagagcgc ggtgtgctgg
       61 tgatcgccag cggcaatgtc gtgcacaatc tgcgcgcgat ggactggagc ctcggtgaca
      121 gcggttatga ctgggccaaa cggttcgacg agcacacgaa ggcgctgatg ctgtccaacc
      181 ccgtcgatgc ggcggggctt gacgagcaca ctgatttccg gactgcggtg ccgacaccgg
      241 accatttcat accgttgctc tatctcgccg gactggctgg cgtggccacc gacaagctcg
      301 acatcctcgt tgacggctac gcgtacggtt cgatgtcaat gaccgcctac accctaggat
      361 tggcgtgtgc cgacatcggg ggcagccagg tcgacacgct gcagccggcc tcggactttc
      421 ccgccgacgc ctccaacatc tgacccgcca gaccagggac agcattgctt aaaaggcggg
      481 tcggcaccga gttcggcggc tcctgtcacc cgctagcggt gcccatccag tcgcagttct
      541 tttcgtcggg aaaagtgttg ttccaggctt gccggtgaag gttgcgcagc tgaccgcctg
      601 taagccgcgt gcggcaagct tggggaatga gtgcttgggc ttcttcgaag ggcagcaacc
      661 cgtcttcgac ggattccgcg agcagagcca gataattctc tacttcgtca gggttcccaa
      721 ctcgggttcg agttgactgg gggaaggagg cgagcagctc ggccactgat gaccggctca
      781 acggcactgg gcagcagctg accccaaaca cccggcttac gccatcctcg gtggcccggg
      841 cacatcctcc cgggttggcc gagtcatact cgcggcacta gtcggacgac catcccggcc
      901 tctgattacg tgcgtcgcgc ctaccgccac catggctcgc cgccagtgtt cagtatcgcc
      961 ctaaccgtcc cgtcaatacc gcatacctgg cccaacccac gcgatcgaca agagtaatac
     1021 gtgcgggcct gatgcactgg gtgatgcagt gggccctgtg gtcttttccg ctcgagcaag
     1081 cagcgacacc gagtaacagc gcgacgaaga cagccgtggc tgaccggacg ggtcggaaaa
     1141 tgggcactga cgctctccgt cactggtcga attgggtccg ctgcagactg aacccatcag
     1201 gttgaacatg gactgaatcc ctgactaaga atgcgttcgg cgtctcagcg tttcttcagc
     1261 tcgacttagc gatgatcccc gcccaaggct gcgcagtctg ctgcagcagt cgcatcgtcg
     1321 gtgcacgcgt cgagagcggt ttctgacaat gtcgtcgtgc gatgggtgtc tcgggcacgg
     1381 tcgccacgat tccgaccgat ggcgccagtg ataccaggcg cagaccgtcg agatcgaacg
     1441 tgctccccgt agtacggctg gccgtaccgg gcagcaagca tctcgatggc gaatcgcggc
     1501 aacgcgctgg tgcggcggcc cgctgtggtc tttggcctgg tcatcgctca cgagcccctt
     1561 gccggtgacg cgcacgacct tgccgcaacg gccaacgttc ctgctgcctc gttgtagtcg
     1621 ctccaccaca gccccagcag ctcgccgcgc cgcaggccag tgacgatcag gatggtgaac
     1681 gggtgaacgg ctgccgtcag ggcatcagcg catagccccc actagcgctg cggcgaccgg
     1741 ggccccctgg gcggcgggcc aggaggccgc aaccgggtcg tcggcggctc cgtcggccga
     1801 ggtgcacgcc cccgcggatc agggacagga ggtcgcggac gcacaggcgc cgggggcctc
     1861 ggcgccggac ccggcctctt cggatggagt gccctgcgcg gcggccgcat ctgcatcgcg
     1921 tgtgtctcgt cgtcagtcca atcccgttgc accagaacgt cttgcgtgat gcgatccaac
     1981 gcctctctga cctggccgcg atccggtcgg acctgaaggc ccgacgattg gctgagccgt
     2041 tgcgtgagcc aatccatcgt ctcggcgcga acgagttcaa cctggtgcgc cccggccgag
     2101 gtcagccaga agacatctcc gcttcgctcg gcatagccgg tggcagcgag gcggtcgaac
     2161 gcgggctcca ggatttcgcg cggcacccgg acatgctcgg cgatgtcgcc cagccgggcc
     2221 tgtcctgcgg cctgcccgaa gcggaagacc tgcagcagcg cccacaaccg gccgatgtcg
     2281 agtttgcttc ccggacgttc ggcgatggcg cgcagctgca tcccggagcc atgacgaatc
     2341 aaccgaccga ccgcgttctc gaccacttgg tcgggcgatg cgcttgtcgg catcgcgaaa
     2401 ccctcgccca tatccgccgc ggtaccgacg tcgttgttgc gcaacggaac ctgcttgagc
     2461 atcaacgcca acacgaaccc gaccaccgcg accggcgccg catacaggaa caccaggtcc
     2521 agggcatcgg cataggccgc cgcgatcggt gcggccattt ccggcggcaa cttgtgcagc
     2581 gcatcgggcg actgcgccgc ttccggcggt gcgccgctgc tcgcgagggc agccgcgagc
     2641 cgagaatgca ggaagttatt gaacaacgag ccgaacaccg cggcgccgaa cgagctgccg
     2701 atggcgcgga agaacgtgac gcccgaggtc gccacaccca ggtcggagaa gtcgacggtg
     2761 ttctgcacga ccagcaccag cacctgcatg cacatgccga tcccggcgcc gagcaccacc
     2821 agatacaccg actgcagcac cgtcgacgtg tccgggccca tccgcgacag cagcacgaaa
     2881 ccgattgcca tgacggcggt accggccacc gggaagatct tgtagcgccc ggtgcggccg
     2941 acgatcacac cgctgctcat cgacatggtc agcaacccga gcaccatcgg cagggtgtgc
     3001 aggcccgacg tggtggccga caccccgtcg acgaattgca tgaacgtcgg caggaacgtc
     3061 aaggcaccga gcattgcgaa gccgacgacg aacgacagcg cgcagcacac agcgaagacc
     3121 ggttcgcgga acagtcgcat cggcagaatc ggttcggcag cgcgcagttc gaccgcgacg
     3181 aacgcgccca acgccaccag cgacccgacg aagagcccga tgatcatcgg cgaaccccac
     3241 gcgtaggtgt tgccgcccca actggtggcc agggtgagcc cggtggcgcc cagcccgacc
     3301 aagacgatgc ccgcgtaatc gatgaccggg cgcgccatcc ggccgagatt gggaatcgcg
     3361 gccaccgcga ccacaatcac cacgacggcc accggcacgt tgatccagaa cgcccaacgc
     3421 cagctcaggt tgtcagtgaa gaatccgccg agcagcgggc cgatcaccgt ggtgacaccg
     3481 aacaccgctc cgagcgcgcc ttggtagcgg ccgcgttcgc gcagcgggat gacctcgccg
     3541 atcaccgccg tcgcggtgac catgatcgct ccgccgccga cgccctgcag agcgcgcgcc
     3601 gccaccagca tcggcaacga gccggccagc ccacacagca ccgaacccgc catgaagaac
     3661 agcaccgcgg cgatgaagac cgtcttgcgg ccgatcagat cgccgagctt gccgacgatc
     3721 gccgtcacga ccgtcgatgc cagcagatag ctggtcacca cccacgcctg gtggcctgcg
     3781 ccacccaggt cggccacgac cgtgggcaag gccgtcgcca cgatcgtctg atccagagcg
     3841 gccagcagca tccccagcag cacggccgtg aaaatcagat tgcgcttcgg cgccgccacc
     3901 tcggcggcac tgggctgcgg tggctccggc ggtcgcggcg gaccggtcat cgttgccgtc
     3961 ataccttcgt ccttgttgat gtcgggtgga ctcgatgccg tatagccact ctctgggatt
     4021 gatacccctc gaccagcgcg cctacccccg acgtcaggga aaggtagcga ccctaatgaa
     4081 atgtatcgga ccaatgcctc gcgtaccccg cggcgtgcgg ctaaacccga tcccaccatt
     4141 cacctccttt tcacagtggc accagcaagg cgaaaagcct tgagctagcc aagaagcaac
     4201 cgtcgatgtc tatctcgacc acgccgacga cgccacacag atctgtggga gcagacctgg
     4261 gatcagctcg aggagctcgc cgtcggcaga ctcaccgcga aggagaaacg gacagtgctt
     4321 cgggttctgg aaggtttaga gaagcgactc tcgccggggg tgcccccagc gtaggaaacc
     4381 cgtcctcggc ggctgcgcat ggagagcggc acaggtcggt taactgttgg gcatgagcaa
     4441 gacgacgtgc ggagtttttt tgccctaagc actcagccgc cctcgcacga tggagcgagg
     4501 agtaagtctc ggacgccagc gaccgaggcg gtcacgctcg ccgctcgacg gaaacctggt
     4561 cgacccgcgg taacgtcgtc catgcggacc tttcggcata tcaggtcagc aggggcagcc
     4621 gccacagcga ctggcccggc tgcagcatcc agagccggct ggcgttgtac cgcaccagac
     4681 tcagcgaccc cccgatcaag accgccgcca acagcgttcc caaccccgcg gtcaaaagcg
     4741 aggtcagcag ctcatcgggg ttggccgtgg tcaagtcgaa ccaggcatca caaatcagca
     4801 ggacgcccgt agtgaaggcg ggaaaaacga gcacctggcg gtgcagccac gtaagaacag
     4861 ccgtggcgat catgaacacc accagcaggc tgtcgaagcc caaccacgtc accggccaat
     4921 cacgcaccgt gtagttctgc ggcagcgtga accccagata cacaatccac ggaatcatga
     4981 acaccgcgcc gccggtcatc aagcccagat gcgcctgccg caaaggcgcc atccagccgt
     5041 gcgaccgcac ctctgccacc ggccgctcga gccgcgtgat gagctcacgg cgctgctcag
     5101 cgctcagcgc accgatttgc gcgtcggaga gcacatcctg gcttgtcgcc gtcacgtcca
     5161 accacctccg gtgaccagtg tgtcgaaaac cacggccttc gtcgatgggc cgtcaacacg
     5221 cgcgcgagcg atgcggcaca aattccagtg acagcagcac cacgagggca cccagaagct
     5281 ggaccgccgg gatcaacaca taacggcgag cacccaaggc gtggaacttg cgcagatacc
     5341 ggtagagcga ctccagtctg atcagcggat cacctaaccg gatcaatgag tagtagatgc
     5401 gttgcaggcc agtccgattc gtctcgtcga acaactctgg aaacgccgcg aacgccagcg
     5461 acaaccggcg tcccccctgt tcccgacacc agtcgatcat gtcgacggtc agccgttcat
     5521 cgactccgtt gggcgcacca gggcgccgcc acggtagatc gagcgacgca tcacttcccc
     5581 cgccggatac gacatagcgg tgaaagccct gcacaatgcc ggcggcatca cgggcgacga
     5641 tcaccagcac accgggatag cgcccggtca acacgccgtc gagcatcatc gaaaagccgc
     5701 gctcgtagcg cccgccgcgc cgcgatgtgg acaccacgtc gagaagttcc tcgcgaagcg
     5761 ccgcgctgag ggactgttcg ggaatgacct cggtggtgat gccgcgcttt cgggtgcgtg
     5821 ccaccgcctg gcgcagattg cgtaacttgc gacccgacag tgcaaattgc gctacgtcga
     5881 ccacgacgtc acggccgacg ggcacagaca tcaacggctg gtgcaccact gatgcgtccc
     5941 gccacagacc aacgcatcgc tccccgcagt tgagcacgac gatgcgccag cccctggtgg
     6001 cacacatcgc tgcgaagtcg cgcaccagcg cacggtagtg cgagggctcc cctaccggat
     6061 ctccgctgac gacagcgaaa cccagccgtg cacgatagct cagcgccgcg gtgtgatcgt
     6121 cagtgaagaa cacagacttc gacgacgcca tcgcgaatgg cgcgagggca tcgccgcggg
     6181 tcgagcaaat cagcggccat acccattccg ctccagggcc aggcggcgaa ggcagcggcc
     6241 acatcaatgc cagccccgac gcggcgacga gcacatcacc gatgatccct gcgctcaaca
     6301 tatgtgcgcc gattccggcg agcaccatgg ccagcgccgc agccgcgtgc ctgccggtga
     6361 ccgggcgccc tagatagacc ccacgagaca gcagcatgct cgcgaccaat aaagacagcg
     6421 accacgccaa ccgcccggag ggttgccagt cggcggcgtc atgatcgcga gcggccatgt
     6481 agcccaccac aaggaagccg ccggccaacg ccacggctcc caatcggtgc gcaccgcgag
     6541 acgatgtcgc gacgagcgtc cgctggctat cgagcgtccg agccaaaacc gctctcgcgc
     6601 cgttgtatgt cgtcgctgcc ctcgcagtca tccgcttcct ccgagacgcc ctaacccacg
     6661 attcccccga ccgatggccg ccaggatagg aatcccctgt ggcgccagcg tcaaacgcag
     6721 tctgagtcaa ccggcgcggt cgagaattta cctgtctgag gacggccagc agcgactcct
     6781 gcggcgcaac taatccgtgt cgcggctatg tcgacgagct ggccagccag cacgtgcgct
     6841 ggcgcggccc caacctttga gccaaccgat aagcggctgt caacagcccg cgcactctac
     6901 gtccgccgca ccaaatatga cgggcgcgtt aacttttcgc gtttctaccg ggacgccagc
     6961 gacctcacag acctattcaa gtgggtttta cggccacggg atgacgatcc cgctatgaac
     7021 agtaatcgtt accctgcaag gggccgcact ctgaaactgg ccgccgcggt cgtgggagca
     7081 gcggccctca ttgctcttac cgtggcaacc acgagtcagt atcgcgacgg ctcaggcacc
     7141 acagccctgg ccgggtccgg ggacgcgccg agcaacacca cctatacgca gcccagcact
     7201 gcagcaatga caatgggtgc aacagctacg tggtcggcgc cgtcatcggc ccctgaggtc
     7261 agcgaagcga gcccggcgat caaggcgggt tcgtaatgga gctagcgcgc agcgtgcacc
     7321 tgcggctggc cgctggtgtc gctgcgctgg ctgccctgtg gatatgggcg gcggcgggag
     7381 gcggcgtggc agacgcggcc acttcggcac ccgacgtgac cggcaagaag tacagcgatg
     7441 cgcagagcgc gatcagcggt gcaggcttca ctccggtcgt gtccaccgtc ttcggcgatc
     7501 gcaacacttg gtccaattgt ttggtcaccg aaacgaagca gcggacggtt cagcctccac
     7561 ccaacagcgg cggctcggcg gtcaagcagg ttctggtatc gctgaattgc gacgctgggg
     7621 tcgcatcggc aacccggccc ggcaactcgg cggccagtcc ggagggacga gcagccacga
     7681 aagcggccgc cgcctcgcct accggctcga gcagctagct gcatcaatca ctcgtaatgg
     7741 ccacgcacag gacgggtaaa cgcggtagcc caacaagatt caccgggcat tcacgtgtgc
     7801 gccggtacgg tcgagagatg cacatcgacc ggcgggacct cctgaaggcg gcggcgactg
     7861 gggtggtcgc ggcgacggcc ggcgccccga cggcgctcgc cgacgccggc cgctcggcgc
     7921 tgccgcgggc acgggtgccc gcaccaaccg gggtcgtaac ccgcctgccc ggcgacggaa
     7981 accaactggc tttgaccatc gacgacggga cgagtaccga cgtcgtcgcc gcgttcgcac
     8041 agttctgccg tgacagcgga acgcgcctta ccttcttcgt caacggagcc aacgcctcgt
     8101 ggcgtcacaa cgcggaggcc ctgcggccaa tggtgaactc cggtcagatc cagctcggca
     8161 accacacctg gtcacacccc gacatcaccc gactgcgcgc cagcgacctg accgatcaaa
     8221 tcagccgcaa caaagacttc ctcgtcaaca cctacggcgt cgacggcacc ccgtacttcc
     8281 ggccaccgta cgggcggcat aacagccaaa ccgaccacgt gcccgccgat ctgggctatc
     8341 ccaccatccc cctgtggagc ggcaccatcg gcgacagccg gcccatcaac gaaaccaacc
     8401 tcatcgccgc cgcgacccag tcgttcacgc cacaggccat cgtcctcggt cacgccaacc
     8461 tgccgaccat cacccacacc tacagccaac tgctcgacat catccaaggc cgcaacctgc
     8521 agaccgtcac cctcgccgac gtcttcagct gaccgccggt caactcctcg actctgccgt
     8581 cgagatttag ctttcgtctc gggtctgatc ctggtcacat ctgcggcggc caggacggta
     8641 tcgttttcca ttctctcgat ccccggcagc catcagcgtt gcggaagctc acagtaatcg
     8701 ggctatcagc gttgctcata tgtacgcaga gcgccttgtt gtcattgttc cggtcaattg
     8761 cgtaagtccg gtgttgcgag gactatcccg gtgtcctgaa caaaacgtag gagtcgctaa
     8821 acctcgtgtc acagtcagca ctgcgatatg acggacatct tctaccggcg atgaaacgat
     8881 agtgacgtgg accccagcgg gaatggtgtc gtggaatgac cgcgaggcgg ctggtctgcg
     8941 gttcgtgcgg caccgaactg ttgctgaact cgaagttctg taacgagtgc ggggcaccgg
     9001 tcacacaggt caaccgatcg gcggaataca agcaggtgac ggtgctgttc gccgacgttg
     9061 ttcattcgat ggacatcgcg gccgcggtgg gtcctgagcg gctgcgcgag atcatggccg
     9121 aactggtcga ctgcgccggt gcggtggtgc gccgctacag cggaaccgtc gacaagttca
     9181 ccggcgacgg aatcatggcg atcttcggcg ccccagtctc ccttgaggac cacgctgtgc
     9241 gtgcatgtct ggcagccctg ggcgttcaga aggaagccaa gcgacttgcc tccgatgtgc
     9301 aagaccgcga cggtgtggag ctgcaattgc ggatggggct gaactccggt caagtgatcg
     9361 ccggcgagat cggttctcgg cctatcggtt acaccgccgt cggtgagcag gtgggtatgg
     9421 ctcagcggat ggaatcgatc gctgcgccgg gcggggtgat gctcagcgcc tccaccgcgc
     9481 gactggttga tggcacagcg gcgctgggcg aaccggagag agtccgcatt aagggcacaa
     9541 acgaaccagt caccgcctac cgcctgttgg gcataggaga ccggcatcgc gctactgagc
     9601 gcggtgaggc gaatctggtc ggtcgccggt gggagatatc tgccgtcgag cgcttgttgg
     9661 accgtgcact cgctggacat ggcgctgtgg ttggggtggt gggtgaaccc ggcatcggca
     9721 agagccgtct ggtgcgcgag gttgccgcga cggctgcggt ccgaggtggg aaggttttca
     9781 gcgccttctg tgagtcacac accagccacg tcccttttca tgccgtgggg cgtctgttgc
     9841 gggccgccac cggcgtcgag ggtcttgacg gacaggctgc ccgcgctcga gtgagagacc
     9901 aatttccaga cgcagacctt gaggatgcgc tgctgcttga tgatctgctc ggtatcgccg
     9961 acctgaatac tgctataccg gcgattgatc ccgatgcgcg tcggcggcgg ttggccgcgc
    10021 tggtgaacgc cgccagattg tcccgccaaa gcccggcgat ctatgtcatt gaggacgccc
    10081 actggatcga tgaggtcagc gagtcgatgc tgaccgattt ccttgccgtg atcccgcaga
    10141 cgccctcgct ggtgctggtc acgtaccgac ccgaatatca cggcgcgttg gcccgagtgg
    10201 ccggtgccca aaccgtaacc cttgcccccc tcagtgattc agaaacggca gcactggtcg
    10261 cacatctcct cggcgcagac ccgtcggttg atgcgctggg ccagagagtc gccgagaggg
    10321 ccgccggtaa tccgttcttt gccgaggagt tggtgcgcga cctggccgag cgcggtgtgc
    10381 tgcgtgggga acctggtgcc tacatatcgg cggcggaggt aggcgcggtg agggtgcccg
    10441 ccacgttaca ggcgaccatt gccgcgcgca tcgaccggct ccatccaaaa gccaagcgca
    10501 cgctgagcgc agcggcggtc atcggctccc ggttcggcct cggccttctc acggtgctgg
    10561 gtgttgagcc ggtcgttgac gaactggtgg cggcccaact cgtcgatcag gtcagctcga
    10621 ccagacaacc ggagtatgtg ttccaccatc cgctcattca ttcggtggcc tacgaagcac
    10681 agctcaaatc ggaccgctcc gaattgcacc gacgggtcgc caccgcggtc gaagcccgcg
    10741 agccggaatc gggcgacgaa aatgcagcgt tgatcgcaaa acatctggag gccgccggtg
    10801 atctgcaagc cggctacggc tggcacatgc gggccgcaac gtgggcgacc aaccgcgaca
    10861 taagcgcggc ccgactgagt tgggagcgag cggccgagat tgccgacgcc ctacccgccg
    10921 aacacccgca ccgggcggcc atgcgcatcg ccccgcgcac catgttgtgt ggaaccggct
    10981 ggcgagtcca cgccaacgtt gccggtgacc gcttcgatga attgcgggag ttgtgcagcg
    11041 ccgccgggga caaggcgtca ctgggcatcg cgacggccgg gttggtggtt ggctgcgcgc
    11101 atcaagaccg ggtgcgcgag gcgtcgcatc tggcatcgga agcctgggcc ctcgccgagg
    11161 cggtcggaga tgcgaccttg acggtagggc tgtcctttcc ggtgatctac ggtcaaatcg
    11221 aaagcgccga gtggagtgac gtgctgcgat ggtcacagac tgttatcgac ctggctgacg
    11281 gtgatctgtc aaaaggcaac tttcttgtcg ggtcgccgtt agcactcgcc tttgcgtcac
    11341 ggggtatggc ccgttatttc ctaggtcgtc cgggatggcc agaagacctc cggcacagcc
    11401 tggctatggc ccggcgcgcc gaccccgcgt cctacgccgg ggttgtcggc tatgtctact
    11461 tgttgggggt accgtttggc gtgctaaggc ccgatgatcc tgcgctgcag gcgatcgagg
    11521 atgctctacg gatcgcagag cgatccagcg atgacgtcgt ggtgggcttc accaaggcga
    11581 cgttggcact tgcgctggtg catcgccaaa acgccgcgga acgtgaccgc gggcaaaagc
    11641 tcggagccga ggtcagccaa gtgttcctaa accaaggaca caacctctgc gatttgccga
    11701 tcgtcgaggt ctacttggca cgcgaaaggg ctaggggcgg agaaatcgat ggcgccatcc
    11761 cgctcatgcg cgccgcctgc gaccatctct tccgcgacgg acgactgctg ctgtggggcg
    11821 ttcccgcgac aggtgtgttg gtggaaacgc tgctcgaccg ctgtgccgac ggtgacgtga
    11881 ccgaagccga ggccgcaatc gagcggttag cggcggcgcc agccgatgag ggtctggtga
    11941 tgcgcgacat ctggctgttg cggctgaggg ctctattggc gcgggctcac cgcgatgacg
    12001 ccgcttacgc acgttttcgg gaccgctacc gcggcatggt ggaatcgctt ggctacgaag
    12061 ggtgtatcgc gtgggtcgat gcgatggcgt gacggcggct actcctttgg gccgttgtgt
    12121 tgcgcaaggt gattgaggcg cattgcggct ttctgttcaa acacaccggt gacgggtatg
    12181 cgacgcgttc gcgtcgccga cgtccgctgt ggatgctgcg gtgcccagca gcggggattg
    12241 gaattgccgg tgcagatgga attggcggca ggcgaggctg aactgcggga cggggactat
    12301 ttcgggacgt gctgaaccgg gcggcgcagg tgaatgcgga aggaggcggc ggtcagatcc
    12361 tggtagccga ctcgacagag gttccgctca gtggcgttga cctgtcgatg tcggtccgag
    12421 acggttgcga gacatgcgga agccgatcac tgttttcagt tgcatgcgcc cggcctgcag
    12481 acggatattc cagcgttgcg ggcgttggat acgagtccgg ggactggtgt ctttgcccgc
    12541 atggcgtcca acgttacctt tcggtggggt acagcaccag catgctagac tcgaaatatg
    12601 tttggtacca aagcattagc gcacatttag tctgttaaat cacaacgcgt cacaatcggt
    12661 tctcgccgcc gcgttccgtg acggaagagt gctgcgcttg gcggcccacg ctcgccgggt
    12721 tttgggatcc cgtcgcagtc gattcgaagc ggcaacacaa tcggtgccga aagaacagtg
    12781 aaccggcgac ggcacggcca agcgccgcgc ccgtcttccg ccccgctgga tctgtcaacg
    12841 agagcttgcg gcgctggccg gagcgtgacc cgtcgacccg caatctggct tgactgtggc
    12901 ggccatacgt cgatggcacc actgtggtac cggcccactg aggggacggc cttcgacgag
    12961 ccgctatccc gcgagctggt tggagttctg aaagagcatg ccgatgtggc gggccgggat
    13021 cggggcgtgt tgaggttcag ggcggtggct gatccgccgg ccggcgatgt gcacactgcc
    13081 gccgtcgggc ttgtccatgc cggccagaca tgccagcagt gttgatttgc ctggcccgga
    13141 ggttccggtg acggcgacga attcgccacg ctccagcctc agcgatactg cttgtagcgc
    13201 aacgggttcc gcgtcaccgg cacggtagaa ccggtacagc gaccgggctt ccagcgccga
    13261 cggtatcgcc gactgtgctg tcaccgccac tgcagtgaat cggtgacggt ccgccatgcg
    13321 tcgacgttgt cggcaccgac cgcgcccgac agggtcagga tcacttcgtg gccgtcgcgc
    13381 cagaactcgt agcgctcgac cgcgtcggtt ccggttttgc cggtgaccgg gttccgaggg
    13441 gaggtgtcct ggtagatgat gaccatggct ggaccggcct tgcgttgcac tggagtcacc
    13501 atcccgagac ggtagccagg ggtggacgaa gcgatggccg gtacttcgtc gatattcacc
    13561 gactcggtat cgggggcggt ggcgaggggg cgtgtctcaa tacgcacggt gttcgacttg
    13621 tcggtgaaga tggtcgcggc ccgtcggtgc tgcgggccca cccttcgggc accgacacgg
    13681 tgaaaagccc gctgggcgcg gtgaacggca cgaacggttg gttgtcgggg gtgtcgccgg
    13741 cagggttgct cttcgatgtc ggcgcccccg cggcagtact actgctgagg gtggccgacc
    13801 ccgacggggc ggtcccgctg gtcccgcacc cggcaagaac cgccgcgatg gccaagaccg
    13861 ctgcgacacc cgacacggtg cgccaccaac caggacgtgg tggccgtgtc gagccttggt
    13921 cacccactcg tggctgtgga ctgcgatcac caacgctgtc agggcggtga gcagcgcggc
    13981 gacgcgcatc gcgacagcga tataacggtg gcgggacagc cggcgggcgc cgaggacgaa
    14041 ggcaatgatg atgaggccgg ccaccaaagt ttcccggcgt ggcgacgacg gcgatcgcga
    14101 cccacactga ctcgttcacc gcgctgaggt cggacatggt tcgagcatgg cggccgatag
    14161 ctttggcaac gctgagaagg aatcgatggc ggccgagcat tctgctctgc tgggatcatc
    14221 ggattggtga acccgcagat gacggcgtct cttgtgcgtg ctattggtcg aggacgagtc
    14281 cgcggcttgc cgagacggtg cgtcgcggcc tggccgccaa gggattcgtc gtcgatgtcg
    14341 aacacgatgg tgtggacgct tgggcggccg ccgagaccct tggaatttcg acgtggtggt
    14401 ccttgacatc atgttgccgg ggctgagcgg atatcacgat gccgccaaca cgggctgaaa
    14461 gagcagacgc acgaagcgcc gggcgttgct gttgaggctg aatccggtcg atggctgctg
    14521 ctcaccgccg tctgtccttc gcagcgccga cgccacccga ctcgcacaga caagttcccg
    14581 gagacgccag ccgaaaagcg cgtgacagca ccgcccataa cgccgcctaa cgtcgaccca
    14641 tccactcgta gttaagtgcg cgggcggcta cagacacaga cacctacgcc gtgtcgtcga
    14701 gttgaacggt gctggctccc tgaaacgacc cgattgccga tcagagcttc gaagtcaggg
    14761 tcctcaaccg tcacaggcgg cgcagtgccg cggcatggat ggcgaccagt gtccgcaacc
    14821 gaaacccagc cacaggggac atcaggctgt aggcagcttg tcgcacgtga ccggcgggtc
    14881 gcgcacccgc actccggtgg caaccagcct cggctgaccc tctacgagcc aggtggttgc
    14941 cggccgtgtg tcgaataaac gtgacggggt ggtccagctg gcatgagtga cacggctcta
    15001 ctcgcggtcg acttgctgaa ctcgtatgaa gacgaagacg ccgacgagct cccagcagcc
    15061 tcgcccagat cgtcgagcca ctggccgggc tgatcgaccg ggtacgcggt cgcgacgacg
    15121 tcgagttgat ctacgtcaac gacaactacg gcgacttcgc cgccacccgc gaggacatcg
    15181 tgcgccaagc cctcgacggt gcggaccccg aactggtcaa atctattctg ccggaccaaa
    15241 actgggcgtt cgtcgacata ctgctgtacc tgcacgggca ccacatctgc gttatccccg
    15301 gttaacgcca ccgagcccac acgtacggca ccagtgatcg aaggaaatcg aggtctggac
    15361 ctggctccgc tccggcaaag gcggcttcaa agtggtcttc ggccaccgag agaatgccct
    15421 cggcgaattc cgccgcgtat tggatgctgc cgtatcgatc catcatgtcc cgaattgtct
    15481 gtacctgatc ggccgtccgg tcgtcgcggc ttctacgcaa gtaatcgcga actatctcag
    15541 catcggccgc ccgagcgttc gcgctaaggt gaacgagagg caacgtccgc ttgccctcgt
    15601 acagatcgcc gagtatctcc ttgccgtaga cggattcatc cccaacgaga ttgagcaagt
    15661 cgtctcgaat ttgaaacgcc gcgccgaaat ggaagccgaa ccgtatcaac agatcgagtt
    15721 cggcagttcc acgggagccc accaacgcac ccacccgcaa tggatggatg gtggtgtacc
    15781 aacacgtctt gtgcatgatc agttccaggt agtcagacgg cgtgaggtct tcgacgttgt
    15841 cgaattgcca cccgacctcg agggcttgcc cttccagcgt tcgcagggcc atggtgtcga
    15901 actccgtcca cacccggtcg gcgagatccc ggtcgaattt ccgtgtggcc tgacgtaaca
    15961 cctgcccggc cacgaccgcc aacccgtcac ctgcattcaa cgccgcagac aatccgtaac
    16021 tcgccgccag cgtcgggcgg ccccgacgca tctcgctacc gtcggcgata tcgtcatgga
    16081 caagaaaagc gttgtgcaac aactcgattg ccaccgcgac accgagcaga tcctcggtgt
    16141 cgccgccgaa agcccgccca gctgacaggc atagcgccgg cctaagtgcc ttgccggctc
    16201 gcgacggata ctcgcgcatc ggcgcgtata accaccggct cggttccccg tctggcattg
    16261 cgtccagcat cgatcgtcga atgagctgtg ccaccttgcg gaggcgctcg tcgacccagg
    16321 gaaccaggtc cacgccgtcg gcggtgcttg tcacgacggc gcagcttcga ccgcgagtat
    16381 caccgccagc cacaaccccg atccgccaga agcgaggagg tttccgcggt acgtgccggg
    16441 agcgacgtct accgcgacac tgacttcgac ggcgattccg cggctgctgc gcggcggcat
    16501 cgccacgatt tgcggcatta cacggacgct atcggacgga atgatgttgc cgtcgtgggc
    16561 caggagctca ctgcagtgca cggtcacgtc gccgaaatcg gctgtcccgc ggttgtgtaa
    16621 ccacacctct gtggtggcga caccgggtgc ggtggtcact agctcgatgc gaccggcggt
    16681 ttcgaccttt tccaggtcca cgacgggttc ttcgggccca gaagaaactt gggcctcggc
    16741 tcccagcagc atctgtccga tgacggacca ccatgatttg agcaaaggcg caacatccgt
    16801 ggcgccgaag atgtctgcgc gctgttcgtt gttcagcggc gcgctcgata ttgtcggcca
    16861 atcggtgccg ttgaggccgc ctcgggcgat tcgaacgaag cggtccacca gttcgctggc
    16921 cgcccgaaac ccttcggcct ggatcgagtt gagcgcacgt gcattggcgg ccggatcgaa
    16981 aaccgagacc caaggcagat cgctgaagcc ggcgtcatcg atcccccgat tcacccgacg
    17041 ccccttcccg gctatctaac cttgcacgtg gcgatttgtc ggtatgctac ctgggcaaac
    17101 agggcgagga gtcgctatgg ggatgctgcg ggatttgatt gaggtttacg gcgcgctcga
    17161 caaggcggtc ggccggtcca atgacccggc ggtcgggaac gcggtcgaca gctttgtgcc
    17221 cgggttctca gcaaactgcg gatggtatga cgagatcgag cgcttcttct tcgggaagaa
    17281 tgctaagagc aacccggatc tgggcgaggc gtgttcgagg atcggcgcgg ccagcaaggc
    17341 cgcggatagc caaagggcag ccagcgcgag tgcaggtgat ggtgaaggag gcggacgcct
    17401 cccgcgccca gctttgacca agccgcagaa gtccaggctg ctcttcgggc tcactgccgc
    17461 cgcccgcgca catcaggccg ccgcgccgaa gatgacgtac atgccggacg actacctcat
    17521 gtacgcatac atggaagcac tcgccgcgct cggcccggac gacgagccct acgacagccg
    17581 gctggggaag gccggcgcaa agttcgaaga catctggaat gaagtcgacg ctcgatgcgc
    17641 gggccgcggc cagttctcac gcctgcgcaa atacgcagcg acaacggcgg gtgtcgtcga
    17701 cccgtcgacg caggacatcc cgctgtgcag cgcgaaagtc gtcaccatcg acggcctgcc
    17761 tgtggtcgtg gtcgacacca acctccattc agacagcagg tcgttccatg acgtagtcga
    17821 aatcgtcaac cccttcaact ggaacgagaa ctatcctgaa ttctttctca gcatggatcc
    17881 ctgcgatcca cccgaacgga ccgacggttg gttccgcgtg ctggagacgg tggggtttcc
    17941 cggcctaggc gggctggaac tgacgactaa cctgaagtat tacccgaagc tgaagctgga
    18001 cgaaggcatc gcccacatcg actacgacct agacgacccg ccgccggggc agggcgacgg
    18061 caaggtgatc gtcgaccgcg gcttcatcaa catcgaagtc gccaatgacg aacgcgaccc
    18121 gaccaagccc ggtgtcagcg cgaagactcg gaaagtggtc cacatcgagg gtctgtcgcc
    18181 ctatgcacag cagcggctgg tctgcctcac cggatacggc acggcttcat cggagtttct
    18241 cctcggcgga aaggccgacg ccgagaaacc gtatccgagg gatttcgtgt attacaaaga
    18301 caaagtgccg caagccgacc ccgaggccga gcccacggaa gtatcgaccc atgccgtcgc
    18361 gacagcggtc aacctctgga ccgagtcggt gcagggcctg gtcagtgatt acttcgattt
    18421 tgcccagaag tggacagacg gccggctcag aatacgcgac gtcacagact acagcactca
    18481 ggtgacggag cggctggtca ccggacccct ggagttttgg gaccgcgtca accagccgcg
    18541 acgcacgggc ggacaatcgg gccacgcccg accgcgtggc ggtcgcggtg gcaaccccga
    18601 atagggcggt gacacatgaa ctctcgcaac atgagaaccc gacaggccaa gcagatgctg
    18661 cgcggctaca tgaacgaggt caacaccatc acggaaaatg cgtcggttga cgacgagact
    18721 ggccgctggg gtctagaccc gtggattcgc atcatccacg agctcgtcga tctccagctc
    18781 cggaccactg cttccataat ccagggggcg ttggccggcc cgtcgtggct ccagcagacc
    18841 tccgaggagt tggacgcgga gaccgtcgac gtaagcccgt ccgctcctta tcctcggatc
    18901 gtcagcgcct ccgcatttgt ccgcgtcggc cagccgacga cgaagattcc tcccgccagc
    18961 ctcgatctgg aaccggacgt attgcccgcg ggggcgacgc agattcagat ccggctgaag
    19021 gattaccgat acgtcggcgc caactacaaa ggcaagataa cgctgaagaa cgcgaatgac
    19081 cccagcgcaa cgcctcaaac tgtcgagatc accgtaggcc tgtgaccgac cgttccacga
    19141 agtccacgat cgacaagttg ctcgaccgcg cgatctcggc gataacccgg ggtgatcgtg
    19201 ccactgccga cgcactagcc ggacaggtgc tggcggtgga ccgcaacaat cccgacgccg
    19261 aagaactgct tgcgaaccct accggcggcg aaatccgtcg tctcaccatg ttgttcgccg
    19321 acctcgttga ttccaccgca ttgtccaccc gaatcgatcc cgagatctac cgcacggtgg
    19381 ttggccgcta tcgcgatctg gtgaccgcca tcgtcaaccg cttcgagggt catatcggct
    19441 ccacccgagg cgacgggctg ttggcggtct tcggccatcc catagcgcac gaaaacgatg
    19501 cgcaccgggc ggtgcaagcc gggcttgaca tcatcaacga agtggctgtg ctgagttggc
    19561 gggtagagcg acgcttcggc ttcgacatcg atgtcagagt gggtgtccac cggggggttg
    19621 tgtatctcga tatcgggcaa gacgacgtct acggccttgg cgccaatctg gccgcacggg
    19681 tgtgcagcct ggccgaaccc ggatcggtcg ccgtgtcgga ggcgatcgaa cacgtggttc
    19741 acgacaggtt cgaactggag gaacgcccgc cggctccgat caagggagtc gatgaggaca
    19801 tcgtccatta ccgggtgatc gccgaccggg acgtcaccgc gatcgcgcga ggcccgctcg
    19861 tcggtcggga aggcgaactc gagtacctca gcgacagttg gaggaaggcc gtcgcaggca
    19921 gtctgtccga cccgtgcatc gccttttgcg gagagggcgg aatcggtaag agtcggcttg
    19981 ccggcgccgc ggtggagatg gccaagcaat ccggtgccgt tgtcctcgac ctgtacgggt
    20041 ccccgttcca cacggatatc ggcctgcgtc cggtaaggag gttgctcgag cgacggtgcg
    20101 gcatccaccg tgggtctgat ccggtggaga gtctgggtaa gctcgccacg gaggtcgagg
    20161 ggtacgggat cagcgccgac gtggcggtgc ccctgctggc gccggtactc ggcatcggcc
    20221 cagagagcgg ctatcgacct acacctgcgc gcgcgggcaa gctatacgac cagatcgccg
    20281 gcgctatcaa ggaatatctg ctggcgtgca ttggcaccgg acccgggctg gtgctggtgg
    20341 aggacattca ctggttcgac gaggacacga tcgagatcgt tcaggccctg ctgcaggaca
    20401 aatcggggcg gctcctcgtc gtcgtcaccg ggcgccaatt gcctgctgcg atcgactgcg
    20461 cacagacata cagcctgggt ccgctcacgg acgcagaagc cgacacgctg atccaggctc
    20521 tgcactttga attgaccccg gcggcgcgag cctcggtgcg ccaacgatgt gacggaatcc
    20581 cgctgtacat cgaagaagtg gtcgccaaac tcaagaactt gtccgaagcg acgggcgaat
    20641 ccgcgcaggt gcccgacacc ttgtacgagg tgttgatggc ccgtctgcag agcagcaggc
    20701 acgcgccgct ggtagtcgag gcggccgcac tcgtcggccg ccacatcgat caccggctct
    20761 tggcatcggc ggtccagttg ggcaccgaaa aggtggacgc gctgctcgac gagttcacgc
    20821 gcggcggggt tttgcagcgg atcggcccag acagttggcg cttccgccac gagttgctgc
    20881 gtgaggtggc cgccgagcta tcgccgccga gtgtgcgccg cagacttcac agccgaatcg
    20941 gggatgcgct ggtcggggca gccgacgaca cggctcccga atgggcactg gtggcacacc
    21001 attacgagcg tgccgaacgt ttcgacgagg ctgtggtggc ctgccagaac gcgtcggcca
    21061 acgcccggca gcgcggcgca ctgatcgaag cacgcaacca cctcacccgc gcgctggaaa
    21121 acgtcgatct gatgccagcc ggtcctacac gtgatcggaa cgaggtcgcc gttcgcctcg
    21181 agcgcggatt cctcacgtcg gtcgcgacag gccacgcgag caccgaggcg gccgccgagt
    21241 tcgagcggtg cctacagctg gtcggcgacg agccaagtag ccagctctac gcgaccttca
    21301 ctggtctgtg gagctattac gccacccgtg gcgatctcca ccgggcaact caattggtcg
    21361 aggcgctgag aaacctgccg gacatgccgg aatggtacga agtggccaac gatgtggtgc
    21421 tgggagtgct ggccactttc cgaggcgaat tccgtccggc gagggcgatg ctggagtcgg
    21481 gcgctacagc actggatcgg atcgggtcgc cgcagatcga aggggcgtgg ttcgcaccaa
    21541 acgatccgtt ggccggcgcg cacacattcc tggggctcct ccgcttcttt gagggcgatc
    21601 tcgctggagc tgagtcagcg ttcgcgcttg tcgaaggcca ttgcaagacg ctggcgttcc
    21661 cgcacggccc gttcacgctc tgctacgggc tggcgatgga gacgatggca cgtgtcgagg
    21721 cgggccaact cgaggaggcc aaccaactcg tcgccgaagt cgccggccgg gccgagcagc
    21781 acggcttcga cgaatgggcg atgatttcgg tctgtaacca ggcctcgttg gccgcgaggg
    21841 ccgcgctggc cgccggcgag acccatgccg ctacgttgca tgcacatatc gcggccatca
    21901 ctgcggtcgt cgacgcatgg cgtggcgccg acatgaagac atttctcggc gtctacgacg
    21961 gattactggc gcggcttctg atcgcggtag gcatgaaaag cgaagctcgc gagcgggccg
    22021 agctcgctct gcagatggcc gacgaaaccg gaatacattt ctacgatgcc gaattgctgc
    22081 gcattcgggc ccacacccac gacgacgcgg atgcccgtca cgcggagctt cggggggcca
    22141 tagtgttggc ccagcaacaa ggccatcctg tatttgaact gcgcgccgct gcagacgttt
    22201 tcgagctggt cggcgagtcc agccggaccg tattggagga cgcgctcagt cacttccccg
    22261 tcgatcagac gtggcccgaa ttggcgcgag cgcgggccct gctcaggtga gcacgcctcg
    22321 cggccgggta gcgattctgg gcgggggcat ggccggtctg agcgccgcgt ggcggctcag
    22381 cgagccaggc tggcgggacc gcttcgagtc gataacgatt tatcaacgcg gatggcgtct
    22441 cggtggcaag gcagcgtcca gcagagggcc acacggccgg atcgaagagc acggattgca
    22501 cgtgtggctc ggctcctacg agaacgcctt tgcgctgttg cgcgagtgct acgccgaact
    22561 cgaccgtcca acaaccgctc cgcaaactcc gatccgaacg tgggaccagg cgctggtgcc
    22621 tgccgacaac ctcgcgatgg ccgatcagtc tggtcgagac tggtcggtgt ggccagggcg
    22681 cttctcgcgt aacgatcagc tgcccggtga ccccgaggca acgggtcgcg aactgacggt
    22741 cgtcgagttt ctccgacgag cgctccagtt ggtactcgac ttcgccgaat cactggacga
    22801 gacagccgga ttggtactct ccgccacttc cgacccgaaa gcgacagaat ggcgtgaagt
    22861 cacccggcgg gcggcattcg ccactatcgc ggctctgagt gcttctccgg gccgacgtgt
    22921 tcttcacgcc ggcctgttgg cgcaatcgct ggaagccatt cggggggccc tcgactacga
    22981 gcgtcggccc gatcacaaac ggttttggtt gatgctggca ctccttgttg ccacggcacg
    23041 cgggctgatc accgacaact tggtgaccga cccccgtggt ttcagggcca tcaatgatga
    23101 ggactacggg cactggattc tgcgacacgg cgctccggtt gaggtgctcg acttcccgct
    23161 tgtgcgtgga atgtacgaca tggtgttcgg ctacgaggac ggtgactaca cacggccgtc
    23221 ctttgccgcc ggactcgcgg tgctcctcac cggcattgca ctctttcagt acaagggcgc
    23281 gtttttctgg aagatgaccg cgggcatggg tgacgtcgtc atcgcaccgc tctaccaggc
    23341 actgcgaaag cgcggcgtgc gattcgagtt ctttcaccgc gtcgaccagt tgcatctcga
    23401 cgagcgccgc ctcgtcatcg acgccatcac agtgggccgt caggttgcca tggccgaggg
    23461 gatcgagaac tacgaaccgc ttacgactgt caagggattg ccagtgttcc ccaaagctcc
    23521 attagcggac cagatcgtgg cgaaggccgg aatcgaggac ctggagtcgc atttcgctcg
    23581 acgggacgac gtggagacca gcgtgctgcg gcgcggtgtg gacttcgatc acgtcgtcct
    23641 ggcgacctcg ctcgggatgg tcgagatggt ggcggcagaa ttgatcgccg accgaccgga
    23701 atggctggag atgacgacgc atctgcgcac cgttgccacc caagccttcc aattatggct
    23761 gcgccccttg gagtcgcaac tgggatggca cctgccgggt gtcacaacca gcggctacgt
    23821 cgccccattc gacacctggg cctcgatgcc tcaaacactc tgggcagaga actggcccga
    23881 cgaagaccgt ccacgcacga tcgcgtattt ctgcggagcc ttcaacgccc agtggccgcc
    23941 ggacggtgac cctgcagact acgtggccag ctgccgtcag caggtcctca ccaacgctgt
    24001 gacatacctc gaccaccatc tgtcgcttta tctgcccggt gcggtcaccg gaaacggatt
    24061 cgtctggaat ctgctcagcg gcgtgaacgg cgaccgtggc gcagccgccc tcgaccgaca
    24121 acatgtcagc gtcaacatcg acccatcgga cagatatgtg cagtccgttc ccggctcgga
    24181 caagtatcga ctccggtccg acgaaagtgg ttacgacaac ctggttctcg ccggtgactg
    24241 gactgactct gggatcaacg cgggctgtat cgaggccgcc gtgatgtcgg ggctgcaggc
    24301 cgcgaatgcg ttactcggcc gcagtcgatt tcaccgtatc cgcggcttct acctaccgtg
    24361 agcagctggt cagctcaact gtgccgtctt gaccgtcacc ccggcggccg gcagtcgcgt
    24421 catcaggacg tcccccattg ctaccgcagg cgtgacgacg ccccgtgtgt ccgacaactt
    24481 gtctcggtcc agcgccagcg ccagcgcgca ctcaccggcc agcaccgagg tggcggtgta
    24541 accggggtcg gcctgctgag ccatggtcgc cacataacgg gctccgctgg tcgtggtggt
    24601 gtatgtctcg accttgtaat gcccacgcgt accttcgtcg tgtccaatcc cggcgcgggg
    24661 catcatccgg tcggcgaagc cgcgaggaag catccgcaag aactcgcccc cataacggga
    24721 ggcattggcg atgccggcgt tgaacatcgc cgcgataaac ggagacagga cagttgagcc
    24781 catggtgatc gtttccgcat accggaaacg ccggccatag gtccacccaa ggagcgcgtt
    24841 gctgcgcctc acacatcgcg tgttgtagag ggccattacg taaccgccgg cccacagacc
    24901 ggtcagctcc ggtgagatct gcgcccccct acacaacggc agatccggct gatcccccaa
    24961 atcgggctcg gctcgtctgt cggggctgag gctgtagggg tcatcgagca tgcgccgcac
    25021 gccggcatcg tccgaggcga tacgcatcaa ttcgaccatc gtcgcaatcg acccgccggc
    25081 ataaccaccg gaataatcac gcaacaccaa cgtcgtgtcg gtcaactcac cggccttgtc
    25141 gtcgatagct ctgcgatgca gtgcgtaagc cgtcaaatcc gaagggacgg agtcgaaccc
    25201 acaggaatgg acgatgcggg agccgctctc gatcgcctgg ctgtggtaga ggtcgatgct
    25261 gttacgtacg aacggcacct caccggtcaa gtcggcataa tcggtcccgg tcgccgcaca
    25321 cgcggcgacg acaggtagtc cgtgcgtggt gtacggcccc actgcgttca ccaccacctg
    25381 tgttcgggcg gccatcgccc gcaacgcctc cggctgtgag acatcggcga cgatcagtcg
    25441 ccattcctgc gcagcggtgc cgagcgaact tcgaacggca tgcagccgat cgagggaccg
    25501 accggccagc gcgacgcgag cctccgcacg cgaattcgcc agatagttgg cggtgagctt
    25561 accgataaaa cccgtggccc cgtacaccac gatgtcaagg tcacgccggc tcgaacccac
    25621 gacggccgac gttacctgtc cttcgcgcgc cgttcgcggt aagccgcaac atgttggcgg
    25681 ttgccgcaca gatcttcaac cgccgcaact cccccacccg gatcaggtcc gccaacgcca
    25741 tcgcgaactc cgcacccatg cgctgcgtgg ggcaccgacg acggcacgtt gcagcttccg
    25801 atcggggaag ctgcctgctg caccaacctc gcggccgcac tggctcactg aatagatatc
    25861 gatggcagtg acgcattcag gtccgggctt gcctggatgt gtttcagcgc agcggtcagc
    25921 gaaggcacga ctgtcaccac ggggtgagcg acgagggcgt gacatttgac catccttctg
    25981 ctggactctt cacgcttatt atccgcagac ttcctccatc gagcgccgcc acccgcttga
    26041 cggcctcgat tggccgacaa aataggtctt gagtggggtc cgcgctcgag cgatacacgt
    26101 cagctagcac ctcgtacagg tagcccagca atatgtagcg cgcggcggac aaggcaggcc
    26161 gttccggcga cgcgcccctt cgatcacccg tgcggtcgaa tagtgctagc catcttggtc
    26221 aataaacacc agacggaact tgccgatctg aatctcatca ccgttggcaa gcaccgatga
    26281 ttgcactggt ttgcggttca cgaaagtacc gttgaggcta ccgatgtcaa caactaggaa
    26341 ctggccgttc tcccttcgga attcggcgtg acggcggctg gcggtgacgt cgtcgagaaa
    26401 gatgtcgcta ttgggatgcc ggccgacaga tgtcgccgcc ctatccagca gaaaccggga
    26461 tcccgcattc gggccgcgct tcattaacaa cacggcaacg tcgctcggca aaccatcgac
    26521 tgcccacatc cccggctcgg cggattcagg cgaagtatcg acctcgccga ggacatcgga
    26581 gcgcagatac atcgttgtct ccgcggcgtt ttcgtcggtt gattggtgga tgtcattgct
    26641 caattagttg tcctttcgac gtgcaggaat cgccactcac aaggcggacc tagaagcgtc
    26701 gcaaccctca tccggttgtc cactgcggtt tgcttgaccc gcgcaccacg agtagtgggt
    26761 tcttccagcg gggcttagtt tcggagaact cgacgggtgg ggccagtcgg acgagttccc
    26821 cgtagggcgt ttgggctgtg atggtgtcag gcgcgaggag ttgatgctgc tcgccggtgg
    26881 gcgtgggggc attcttgtcg actttgccca gggacatgaa ccacattgcg cagcgggcc
//
LOCUS       DASYLX010000057         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1429299,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000057 DASYLX010000000
VERSION     DASYLX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429299"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGP27_01535"
     CDS             complement(<1..365)
                     /locus_tag="VGP27_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV7850030.1"
                     /translation="MPERPPNRPPATVTVIKLAWCCLLASVVAAGLMFPVVGGFGLMS
                     NRASDVVANGSAQLVEGEVPGVSTMVDAKGNVIAWLYSQRRFEVPGDQIANTMKLAIV
                     SIEDKRFAEHNGVDWKGTLT"
     gene            656..1012
                     /locus_tag="VGP27_01540"
     CDS             656..1012
                     /locus_tag="VGP27_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7850031.1"
                     /translation="MSSTRPVARRTTTTSLPTSLVQGVEAEARIAWVSQARCRQADPD
                     ELFVRGAAQRKAAVICRHCPVISECGADALDNRVEFGVWGGMTERQRRALLKQHPEVV
                     SWADFFAAQRKHRSAV"
     gene            complement(1120..2250)
                     /locus_tag="VGP27_01545"
     CDS             complement(1120..2250)
                     /locus_tag="VGP27_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEV7850032.1"
                     /translation="MSTTPPPLDMASILSDRSNRVVVCCGAGGVGKTTTAAAMALRAA
                     EYGRTVVVLTIDPAKRLAQALGIKDLGNTPQRVPLAPEVSGELHAMMLDMRRTFDEMV
                     IQYSGPERAQAILDNQFYQTVATSLAGTQEYMAMEKLGQLLAEDRWDLVVVDTPPSRN
                     ALDFLDAPKRLGGFMDSRLWKLLLGPGRGIGKLVSGAMGLAMRALSTVLGSQMLSDAA
                     AFVQSLDATFGGFREKADRTYELLKRRGTQFVVVSAAEPDALREASFFVDRLSQERMP
                     LAGLILNRTHPTLTSLHADKAAEAADVLAAEDPESLTAAVLRIHAARAGTAKREVRLL
                     SRFTGANPHVSIVGVPSLPFDVSDLEALRAIADQITGEASAA"
     gene            complement(2282..3301)
                     /locus_tag="VGP27_01550"
     CDS             complement(2282..3301)
                     /locus_tag="VGP27_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEV7850033.1"
                     /translation="MATEANDRKTVGWPSRLTKARLHFVTGKGGTGKSTIAASLALAL
                     AAGGRKVLLVEVEGRQGIAQLFDVPPLPYEEVKIATADGGGTVNALAIDIEAAFLEYL
                     EMFYNLGLAGRAMRRIGAVEFATTIAPGLRDVLLTGKIREIVTRAEKGKHPVYDAVVV
                     DSPPTGRIARFLDVTKAVSDLAKGGPVHSQAESVVKVLHSDLTAIHLVTLLEALPMQE
                     TLEAIDELQDLGLPIGSVIVNRNIPAYLAPDALAKAAEGDVDADQVRGDLTKTGITLS
                     DSDFAGLLTEAIEHATRIAARAESAEQLEEIEMPRLELPAIPDGVDLGSLYELAEALN
                     NQGVR"
     gene            3350..3511
                     /locus_tag="VGP27_01555"
     CDS             3350..3511
                     /locus_tag="VGP27_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEV7850034.1"
                     /translation="MSETTRWEYATVPLLTHATKQILDQWGEDGWELVSVLPGPTGEQ
                     HVAYLKRPK"
     gene            3508..3966
                     /locus_tag="VGP27_01560"
     CDS             3508..3966
                     /locus_tag="VGP27_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV7850035.1"
                     /translation="MTDWSIRLSELGIELPPVVKPVAAYVPAVRTGNLVYTAGQLPMV
                     AGELPATGSVGGGVAPEEAKALARICALNALAAVDSLVGIDAISRVVKVVGFVASAPG
                     FHGQPGVVNGASELFGEVFGDAGAHARSAVGVAELPLNAPVEVELIVEVA"
     gene            4053..4823
                     /locus_tag="VGP27_01565"
     CDS             4053..4823
                     /locus_tag="VGP27_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7850036.1"
                     /translation="MQHPAYGVLRPVTETASVLLCNNPGLLTLDGTNAWVLRGRGSEE
                     MVIVDPGPDDDEHIAKIADLGAIPLVLISHKHEDHTGGIDKLVERTGAVVRSVGSGFL
                     RGLGGPLTDGEVIDAAGLRITVIATPGHTADSLSFVLDDAVLTADTVLGQGTTVIDSE
                     DGALGDYLESLKRLQGLGHRKVLPGHGRDLDDLEAVTARYLAHREERLDQVRDALRTL
                     GAAATARQIVEQVYTDVDEKLWDYAEWSVQAQLNYLRS"
     gene            complement(4894..>5212)
                     /gene="crp"
                     /locus_tag="VGP27_01570"
     CDS             complement(4894..>5212)
                     /gene="crp"
                     /locus_tag="VGP27_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEV7850037.1"
                     /translation="AEQLLRVLARRLRRTNNNLADLIFTDVPGRVAKQLLQLAQRFGT
                     QEGGALRVTHDLTQEEIAQLVGASRETVNKALADFAHRGWIRLEGKSVLISDSERLAR
                     RAR"
BASE COUNT          867 a         1726 c         1753 g          866 t
ORIGIN      
        1 gtcagcgtgc ccttccagtc gacgccgttg tgctcggcaa accgtttgtc ctcgatcgag
       61 acgatggcca gcttcatcgt gttggcgatc tggtcgcccg gcacctcgaa ccggcgctgc
      121 gaatacagcc acgcgatgac gttgcccttg gcgtcgacca tcgtcgacac gccgggcacc
      181 tctccctcga cgagttgggc cgagccgtta gcaacgacgt cggaggcccg gttcgacatc
      241 aggccgaacc cgccgacgac cggaaacatc aaacccgcgg ccactacgct ggccaacagg
      301 caacaccacg ccaacttgat gaccgtgacc gtcgccggcg gtcgattcgg cgggcgctcc
      361 ggcatgccct acagagtaac taggttgtcg ccgtgtcttc gcccggcagc aggattgcca
      421 gcacaaacgc cgtcacggct catcgagggg tgctgccgct tcggcgagtc tggacggttg
      481 tcccaaaaaa ggggcaaaca aactgttgcg taaatagcgt gtgaccacct agcttgtacc
      541 tacagtgcga ttcaggtaac aaagacctcc gcaatgtggc gtagatcgca tcagcgttct
      601 acaccaaggg actgagccgt caagtaacgg cggctggact taaggggaaa cgctggtgtc
      661 gagcacacgg ccagtcgcgc gcaggacaac taccacctct cttcccacta gtttggtcca
      721 aggcgtagag gccgaggccc gtatcgcatg ggtttcccag gcccggtgtc ggcaagccga
      781 ccccgacgag ttgttcgtcc gaggtgccgc tcagcgcaaa gccgcagtga tctgcaggca
      841 ctgcccggtg atttccgaat gcggggccga cgccctcgac aaccgcgtcg agttcggcgt
      901 gtggggcggg atgacggagc gccagcgccg cgctttgctc aagcagcacc ccgaggtggt
      961 gtcctgggcc gacttcttcg ccgcccagcg caaacaccgc agcgccgtct aagaggaaac
     1021 gccgctccgg ccacaatcgc ggctccatct gatcgtcgtc cggctcgatc tgatggccgg
     1081 actcctcaac gcggcaggcg ccgcttgatc gtcgtccggc tatgccgcgg aagcttcgcc
     1141 ggttatctga tcggcgatgg cgcgcagcgc ctcgagatcc gaaacgtcga acggcaacga
     1201 cggcaccccg acgatggaca catgcgggtt ggctcccgtg aaccgcgaca gcagccgaac
     1261 ctcgcgcttg gcggtgcccg ccctggcggc gtgaatccgc agcaccgcgg cggtcagcga
     1321 ctcggggtcc tcggcggcca gcacgtcggc tgcttccgcg gccttgtcgg catgcagcga
     1381 cgtcaacgtc gggtgggtcc ggttcaggat cagcccggcc agcggcatgc gctcctgcga
     1441 aagccggtcg acgaagaacg acgcctcacg cagagcgtcc ggttcggccg ccgacaccac
     1501 gacgaactgt gtgccgcgcc gcttcagcag ttcataggtg cggtcggcct tctcccggaa
     1561 tccgccgaac gtggcgtcca gcgactgcac gaaagcggcg gcgtcggaga gcatctgcga
     1621 gccgagcacc gtcgacagcg cgcgcatggc aagacccatg gcgccggaca ccagcttgcc
     1681 gatgccacgg ccggggccga gcagcagctt ccacagccgg ctgtccatga agccaccgag
     1741 ccgtttcggc gcgtccagga agtccagcgc gttgcgcgac ggcggcgtgt cgacgacaac
     1801 cagatcccac cggtcctcgg ccagcaactg gccgagcttc tccatcgcca tgtactcctg
     1861 cgtgcccgcc agcgaggtgg caaccgtctg atagaactgg ttgtccagga tcgcttgtgc
     1921 gcgttcgggt cccgagtact ggatcaccat ctcgtcgaac gtgcgacgca tgtcgagcat
     1981 catcgcgtgc agctcgccgg acacttcggg ggccagcgga acgcgctgcg gcgtgttgcc
     2041 caggtccttg atgcccagcg cctgggccag gcgtttggcc gggtcgatag tcagcacgac
     2101 caccgtccgg ccgtactcgg cggcgcgcag cgccatcgcc gcggcggtgg tggttttgcc
     2161 gacaccgcct gcgccgcaac agactacgac gcgattggac ctgtcggaca ggatcgaggc
     2221 catatccagg ggcggcggcg tggtgctcat ctcacgctcc ttctggtccg actcatttcg
     2281 ttcatcggac gccttgattg ttgagcgctt cggccaattc gtagaggctg ccgaggtcga
     2341 ccccgtcggg gatggcgggc agctcgagcc gcggcatctc gatctcctct agttgctccg
     2401 cgctctcggc acgcgccgcg atgcgggtgg cgtgctcaat ggcttcggtt agcagaccgg
     2461 caaagtcgct gtcggacagc gtgattcccg tcttggtcag atcgccgcgc acctggtcgg
     2521 cgtcgacgtc gccctcggcc gccttggcga gggcatcagg tgccaggtac gcggggatgt
     2581 tgcggttgac gatcacgctg ccgatgggca gccccaggtc ctgcagctcg tcgatcgctt
     2641 cgagcgtctc ctgcatcggc agcgcctcga gcagcgtcac cagatggatg gccgtgaggt
     2701 ccgaatgcag caccttgacc acgctctcgg cctgcgagtg caccgggccg cccttggcga
     2761 gatccgaaac agccttggtg acgtcgagga agcgcgcgat ccggccggtg ggcggggagt
     2821 cgaccacgac cgcgtcgtag accggatgct tacctttctc cgcgcgggtg acgatctccc
     2881 ggatcttgcc ggtgagcagc acatcgcgta ggccgggcgc gatcgtggtg gcgaactcga
     2941 cggcgccgat gcggcgcatc gcccggcccg ccagcccgag gttgtagaac atctccaggt
     3001 actcgaggaa ggccgcttcg atgtcgatgg ccagcgcgtt gactgtgccg ccgccgtcgg
     3061 cggtggcgat cttcacttcc tcgtaaggca gtggcggaac atcgaagagt tgcgcaatgc
     3121 cttggcgccc ttcgacttcg accaacagca ccttccggcc gcccgcggcc aacgccagcg
     3181 ccagcgatgc ggcgatggtg gacttgcccg tgccgccttt gccggtgacg aagtgcagcc
     3241 gcgccttggt cagacgcgag ggccagccga ccgtcttccg gtcgttcgct tcggtagcca
     3301 cccttgcatg ctaacgaaac ctgtgaggcc gcccgataag ctcggccaca tgagcgaaac
     3361 cacccgctgg gagtacgcca cggtcccgtt gctgacacat gccaccaagc agatcctcga
     3421 ccagtggggg gaggacggct gggagctggt ctcggtgctg ccaggcccca ccggcgagca
     3481 gcacgtcgct tacctgaagc ggccgaagtg accgactggt ccatcaggct gtccgaactg
     3541 ggcatcgagc tgcccccggt cgtcaaaccg gtagcggcgt acgtgcctgc ggtgcggacc
     3601 ggcaatctcg tctacaccgc ggggcagctg ccgatggtcg ccggtgaact tcctgcgacc
     3661 gggtcggtgg gcggcggggt ggcacccgag gaggccaagg ccctggccag gatctgtgcg
     3721 ctcaatgcgc tggccgccgt cgactcgctt gtgggtatcg atgccatcag ccgcgtggtc
     3781 aaggtcgtcg ggttcgtcgc atccgcgccc gggttccacg gtcagcccgg cgtcgtcaac
     3841 ggcgcgtcgg agcttttcgg cgaggtgttc ggtgacgcgg gcgcgcacgc gcggtccgcg
     3901 gtcggggtgg ccgaactgcc gctgaacgca cccgtcgaag tcgaactgat cgtcgaggtc
     3961 gcgtgagcga cgatcgggca gaggtggcgt gagcgacgat cgggcagagg tcgcgtgagc
     4021 gacgatcggg cagaggtggc gtgagcgcgg ccgtgcagca tcccgcctac ggagtgttgc
     4081 gcccggtgac cgagacggcg tcggtgctgc tgtgcaacaa ccccgggctg ttgacgctgg
     4141 acggcacgaa cgcgtgggtg ctgcggggca ggggcagcga ggagatggtg atcgtcgacc
     4201 cgggccccga cgacgacgag cacatcgcca agatcgccga tctcggcgca atcccccttg
     4261 tgctgatcag ccacaagcat gaagaccaca ccggcggcat cgacaaactc gtcgagcgca
     4321 ccggcgcggt cgtccgctcg gtcggaagcg ggttcctgcg tggcctcggt ggaccgctga
     4381 ccgacggcga ggtcatcgac gccgccgggt tgcgaatcac ggtgatcgcg acgccggggc
     4441 acaccgccga ctcgctgtcg ttcgtcctcg acgacgccgt gctgactgcg gacaccgtgc
     4501 tcgggcaggg cacgacggtg atcgactccg aagacggcgc cctcggcgac tacctcgagt
     4561 cgctgaagcg gctgcagggc cttggccacc gaaaggtgct cccggggcac ggccgcgatc
     4621 tcgacgacct cgaggccgtg actgcgagat acctggcgca ccgcgaggag cggctcgatc
     4681 aggttcgtga tgcgctgcgg acacttggcg ccgccgcgac cgcgcgccag atcgtcgaac
     4741 aggtctacac cgacgtcgac gaaaagctat gggactacgc cgaatggagc gttcaggccc
     4801 agctcaacta cttacgttcc tgacgcccaa actcacgttt cggcgtaaaa gtgcgagtag
     4861 agatcgccaa aatgtcgatc tcggcgcaac gggtcagcgc gctcggcggg ccagtcgctc
     4921 gctgtcggat atcagcacgc tcttgccctc gagccggatc cacccgcggt gggcgaaatc
     4981 ggcgagtgct ttgttgacgg tctcccggga ggcgccgacc aattgggcga tctcctcctg
     5041 cgtgaggtcg tgggtcaccc gcagtgcacc gccctcttgc gtgccgaagc gctgtgccag
     5101 ctgcagcagc tgcttggcga cgcggccggg gacgtcggtg aagatcagat cggcgaggtt
     5161 gttgttcgtg cgacgcagcc ggcgggcgag cacccgcagc agctgctcgg cg
//
LOCUS       DASYLX010000058         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3513780,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000058 DASYLX010000000
VERSION     DASYLX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513780"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VGP27_01575"
     CDS             complement(<1..1119)
                     /locus_tag="VGP27_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850038.1"
                     /translation="MTTSAEGLQQSGGVEMRVHGVGDHGVFSALGNPVYTGPVADRVR
                     IGSLPLIPKHELKLIHWSRPSRKITRNLSWYLAYPFTLINMAGFMGPQRSENPAERRL
                     WWFMRTAIVISSLMMTFAMAMWMSVILETAWRLLGGVPAGLAGIVECLFGPGLLACVV
                     VNRMLRGRPLVDQANRGVALLTLGVLLGTAGLLAARATVGSGMLDYGDATVGDPMALM
                     VGVTTAIAWGFAITLCWIAWRAEGRRKGYGRLSFRPSPDRTALAGAGMLIAFAVAILH
                     TAGSGLRLVLQTILTYTPPVIERRAQGIQDSSHVLPIDLIPVLFLMMAAVFGALFWWE
                     YRRRKPMCAIGVARPRGLKEPVPSHVLLRDTHEFLGRTG"
     gene            complement(1448..3172)
                     /locus_tag="VGP27_01580"
     CDS             complement(1448..3172)
                     /locus_tag="VGP27_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEV7850039.1"
                     /translation="MTASSPLTSPPSESTLRTGTVLQGVPVVAGVQYGPVIRPGRLPV
                     LDNVDAAPEVEEEDRPAEAARFTTAANAVATRLRDRAAHATGAASEVLAATATLAQDR
                     AWLGAAEKLINAGAPAPRAVVAAVDQFVDLFTQLGGLMAERVTDLRDIRDRVIAELSG
                     LPEPGVPLPDVPSVLLADDLAPADTAGLDPALVLALATTLGGPTSHTAIIARQLGLPC
                     VVAVDGLDAIGAGAMVLVDGTRGTVTLSPEPAAASEAVAEARHEVELAAGWTGPGATA
                     DGHAVAVLANVQDGAAARSARDTPAEGVGLFRTELCFLNRDTEPGVDEQAQIYGDVLE
                     AFAGHKVVIRTLDAGSDKPLKFAGHPSDTAEANPALGVRGIRIAEGNPGLLERQLDAI
                     AAAAERTGNPPWVMAPMIATADEAKRFAAQVRRRGLTPGVMIEVPAAALLADRILEHV
                     DFLSIGTNDLAQYTMAADRMSADLATLTDPWQPAVVALVAMAVRAGAAVGKPVGVCGE
                     AAADPLLACVLTGLGVTSLSAAAAAVNGVGAKLSQVTLQQCRDAADAVLATASAGDAR
                     AAAMAILG"
     gene            3276..>3935
                     /locus_tag="VGP27_01585"
     CDS             3276..>3935
                     /locus_tag="VGP27_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV7850040.1"
                     /translation="MYAEERQQAIASLVISQGRASVSELAEAYDVTTETVRRDLAVLD
                     RAGLVRRVHGGAVPVRALRLVEPGVGERENTRAEHKDAIATAATEFFPLSGASVLLDA
                     GTTTSRIAAALPSDRDLNVVTNSVPIAARLAGMPSISLQLLGGRVRGLTQAAVGEQVL
                     RVLDTLRVDIAFIGTNGISVRHGLSTPDSEEAAVKRAMIASANYVVVAADSSKVGRED
                     FI"
BASE COUNT          647 a         1356 c         1348 g          584 t
ORIGIN      
        1 cccggtacga ccgaggaatt cgtgggtgtc ccgcagaagc acatggctgg gcaccggctc
       61 tttcaggccg cgcggccggg cgacaccgat ggcgcacatc ggcttgcgcc gccgatactc
      121 ccaccagaag agcgcgccga agaccgctgc catcatcaag aacagcacgg ggatcagatc
      181 gatcggcagc acatgggaac tgtcctggat gccctgtgcc cgtcgctcga tcaccggcgg
      241 tgtgtaggtg aggatcgtct gcaataccag tcggagtccg gagcccgcgg tgtgcagaat
      301 cgccacggcg aaggcgatga gcattcccgc gcctgccagt gcggtgcggt ccgggctggg
      361 ccgaaagctc agccggccgt agccttttcg acggccctcc gcccgccagg cgatccagca
      421 cagcgtgatt gcgaagcccc acgcgatcgc agtggtgacg cctaccatga gggccatcgg
      481 gtcaccaacg gtggcgtcac cgtagtccag catcccgctg ccgaccgtgg ctctggccgc
      541 cagaaggcct gcggtgccga gcagcacccc gagggtcagg agtgcgaccc cgcggttcgc
      601 ctggtcgaca agcggccgac cgcgaagcat ccggttcacg acgacgcagg cgagcaagcc
      661 gggaccgaag aggcattcga caatgccggc gaggccggcg ggcactccgc cgagcaaccg
      721 ccatgcggtt tcgaggatga cggacatcca catcgccatt gcgaacgtca tcatcaggct
      781 cgagatcacg atggcggtcc gcatgaacca ccacagtcgc ctttcggcgg ggttctcact
      841 gcgttgcggg cccatgaagc cggccatgtt gatcaacgtg aacggatacg ccagatacca
      901 actcaagttg cgggtgatct tcctgctcgg ccggctccag tggatcagct tcaactcgtg
      961 tttcggaatc agcggcagac tgccgatgcg cacccggtcg gccaccgggc ccgtgtacac
     1021 cggattgccg agagcgctga acaccccgtg gtctccgact ccgtggacac gcatctccac
     1081 tccaccggac tgctgtagtc cttcggcact tgtggtcatg tcgggtcggt cctcttggcg
     1141 cgcgaaagtt cttttgcttc atcaggccca gacccacgga ccgatagcct cagcgaaccc
     1201 ggctccacac ccgatgttgg aggaattatg cgcgcaatcg cccgcatccg ctgctggttc
     1261 cgcaaattgc atcaaggcgg ctaaatgggg cgcttgccgc tgggattggg cgatatccag
     1321 ctgaatcggc aacttgcata acggtgctag ttactcgccg gttaactaag cggtacgcgg
     1381 ccggcgcggg cagcgcagcg gctgcaatgg tgcaccacct gcgatgatcg gcaaattagc
     1441 gcagcggtca gccgaggatt gccatggcgg ccgcgcgggc gtcacccgcg cttgccgtgg
     1501 ccaacaccgc gtcggctgca tcgcggcact gctgcagcgt cacctgcgac agcttggcgc
     1561 cgacaccgtt gaccgccgct gccgccgccg acagcgaggt gaccccgaga ccggtcagca
     1621 cacaggccag cagcgggtcc gcggccgcct caccgcatac gccgaccggc ttgccgacgg
     1681 ccgcgcctgc gcgcaccgcc atcgcgacca gagcgaccac cgcgggctgc cacgggtcgg
     1741 tgagcgtggc cagatcggcc gacatgcggt cggccgccat cgtgtactga gccaggtcgt
     1801 tcgtgccgat cgacaggaag tcgacatgct cgaggatgcg atcggccagc agcgccgcgg
     1861 cgggcacctc gatcatcacg cccggcgtga ggccgcggcg gcggacctgc gcggcaaagc
     1921 gtttcgcctc gtcggcggtg gcgatcatcg gcgccatcac ccatggcggg ttgccggtcc
     1981 gctcggccgc cgcggcgatc gcgtccagct gccgctccag cagccccggg tttccctcgg
     2041 cgatgcgaat cccccgcaca cccagcgccg ggttcgcctc ggcggtgtcg ctgggatgcc
     2101 ccgcgaactt cagcggcttg tccgagccgg cgtccaaggt cctgatgacg accttgtggc
     2161 cggcgaacgc ctccagcacg tcgccgtaga tctgcgcctg ctcgtcgacg ccgggttcgg
     2221 tgtcgcggtt gaggaagcac agttcggtgc ggaacaaccc gacgccctcg gctggagtat
     2281 cacgggcgct gcgcgcggcg gcgccgtcct gcacgttggc gaggaccgcg acggcatgcc
     2341 cgtccgcggt ggcgccgggg ccggtccatc ccgcagccag ttccacctcg tggcgtgctt
     2401 cggccaccgc ttccgacgcg gcggccggct cgggcgaaag ggtgacggtt ccgcgggtgc
     2461 cgtcgacgag caccatcgcg cccgccccga tggcgtcgag tccgtcgacg gccaccacgc
     2521 agggaagacc gagttgacgg gcgatgatcg cggtgtggct ggtcgggccg cccagtgtgg
     2581 tggccagcgc caagacgagc gccgggtcca ggcccgcggt gtcggcaggc gcgaggtcgt
     2641 cggccagcag aaccgagggc acatcgggca atggcacgcc gggctcgggc aggccgctga
     2701 gctcagcgat gaccctgtcg cggatgtcgc gcaggtcggt gacgcgttcg gccatcaggc
     2761 cgccgagctg cgtgaagagg tcgacgaact ggtcgacggc cgcgacgacg gcgcgcggcg
     2821 cgggggcgcc ggcgttgatg agtttctcgg cggcaccaag ccaggcccgg tcctgcgcca
     2881 gcgtggctgt cgcggccagg acctccgacg ccgcaccggt ggcatgcgcg gcgcggtcgc
     2941 gcaggcgggt ggcgacggcg ttggccgcag tggtgaagcg cgccgcctcg gccgggcggt
     3001 cctcctcctc gacctcgggt gccgcgtcca cgttgtcgag cacgggaagg cggccgggcc
     3061 ggatcaccgg cccgtactga actccggcga cgaccgggac gccctgcaga accgtgcccg
     3121 tgcggagggt gctttccgac ggcggtgacg tgagcggcga tgaggcggtc atgtgaagca
     3181 gattacaaac gaaccgttga caagtcaaca cgttcagggg taaaacaaca tatatcaaca
     3241 ccgatacggg ccagaaccca caaaggcgga gcgccatgta cgccgaagaa cgtcagcaag
     3301 cgatcgcctc gctggtgatc agccagggtc gcgcctcggt gtccgagctg gccgaggcct
     3361 acgacgtcac caccgagacg gtgcgtcgtg acctcgcggt cctcgaccgc gccgggctgg
     3421 tacggcgggt acacggcggt gccgtgcccg tgcgggcgct gcgcttggtc gagcccggcg
     3481 tcggggagcg cgagaacacc cgcgccgagc acaaggacgc catcgccacg gccgcgaccg
     3541 agttctttcc actcagcgga gccagcgtgc tgctcgacgc gggaaccacc accagccgga
     3601 tcgccgccgc gctgccgtcc gaccgcgatc tgaacgtcgt gaccaactca gtgcccatcg
     3661 cggcgcgcct ggcaggcatg ccgtcgatct ccctccaact gctcggcgga cgggtgcgcg
     3721 gcctcaccca ggcggcggtc ggcgaacagg tgttgcgcgt gctcgacacg ctgcgcgtcg
     3781 acatcgcctt catcggcacc aacggcatca gcgtcaggca cggcctgtcc accccggaca
     3841 gcgaagaggc cgccgtaaag cgcgcaatga tcgccagcgc caactacgtt gtggtggccg
     3901 ctgattcatc gaaggtcggc cgtgaggact tcatc
//
LOCUS       DASYLX010000059         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_771250,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000059 DASYLX010000000
VERSION     DASYLX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771250"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VGP27_01590"
     CDS             complement(<1..1278)
                     /locus_tag="VGP27_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850041.1"
                     /translation="MTPNIHTIIRQHVSLEVRCIDRVYLHAYMPKLQTSGGLCYFLHD
                     YLGYPVPSPALLKPRHDRFVAAVQQFAATQRLEVVPFERGESKDERVAKDRSRFTARD
                     GVVFIGVAQEKMRSFKAHQRPGRGRTPVFDFARQSVAVNHYYFYVHDQDWGPAFLKIG
                     TYMPYPVKLCLNGHEWVKQRLRRDHIPFASLDNGFLSCADPAALQAACDALGPADVQA
                     FFDRWLQRLPWPMTPDERAAGYDHRLALCQLEVSLTQVFDRPVQGRHFFEAVIRENLD
                     LGRPDRVRLLFPLRITRATPPPAFGYRTRVITDGVEPSLHVEYKSSHVKQYFKEQHAL
                     RTETTINNPMDFYVRKAVHNLAHLRDLGDQVNRKLLEVERVSHQCVLTQEALDRLQCP
                     TVEINQRASALRFGDPRVMALLQALTGFTHLPRG"
     gene            complement(1491..2876)
                     /locus_tag="VGP27_01595"
     CDS             complement(1491..2876)
                     /locus_tag="VGP27_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV7850042.1"
                     /translation="MSMGTRDPDQSPLWIAASDLPASPGHPFYTRVNAILDAYGFDRF
                     VEDQCRRFYAAVLGRPSLPPGQYFRLLLVGYFEGIDAERGIAWRAADSLAIRSFVRLA
                     LQEAAPDHSTISRTRRRIDVETHRAVFTWVQQRLVEVGLLNGKTVAVDATTLEANAAM
                     RSIVRRDTEETYQEFLTRLAQASGIETPTREDLARLDRKRKKKTSNKDWTNPHDSDAK
                     VTKMKDGRTHLAHKAEHAVDLETGAIVAVTVQGADQGDTTTIVATVTAAVEQIEAAQA
                     GVDDPQPLEEIITDKGYHSNRIMVELAALGVRSYVAEPARGRRDWSQEPEARAPVYGN
                     RRRIRGPRGRRLMRRRGAYVERAFAHVYDTGGMRRTHLRGHTNILKRLLIHAGGFNLG
                     LMMRHVIGLGTPRGLQGRLAAVLALVVALWTCAVELLRVHTTPSADHRSALTPNRGFE
                     LLPVGALEGAL"
     gene            3107..3661
                     /locus_tag="VGP27_01600"
     CDS             3107..3661
                     /locus_tag="VGP27_01600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV7850043.1"
                     /translation="MAEVSVNNGVNVGALLAAREALKGAPQAAKFTWRASCKWQNGTH
                     SVSKVEGFHGLGEEQKHKTEFSFEADHPEVFASEDLGATPVEYVLVGLASCLTAGVAS
                     VAQNRGVQLRSVEARLEGSMDIQGILGIDSDVRNGYDDIKVTFNIDADASKKEIEAIV
                     AQSQKRSAVFDIIANPTTVSVVVA"
BASE COUNT          666 a         1183 c         1231 g          647 t
ORIGIN      
        1 tccacggggc agatgggtga agccggtgag ggcctggagc agcgccatga cgcgcgggtc
       61 gccgaagcgg agggcggacg cgcgttggtt gatttcgacc gtcggacact ggagccggtc
      121 gagcgcctct tgcgtcagga cacactgatg actgacgcgc tcgacttcca ggagcttgcg
      181 attgacctgg tcgccgaggt cgcgcaggtg ggccagattg tggacggcct tccgcacgta
      241 gaagtccatc gggttgttaa tcgtcgtttc ggtgcggagt gcatgctgct ctttgaagta
      301 ctgcttcacg tgggacgact tgtactccac gtggaggctc ggctccacgc cgtccgtgat
      361 gacgcgcgtc cgatacccaa aggcaggtgg tggtgtggcg cgggtgatcc ggagcggaaa
      421 gagcaatcgc acgcgatcgg gccgccccag atccagattc tcgcggatga cggcttcaaa
      481 gaagtgtcgg ccttgcaccg gccggtcgaa gacctgagtc agactgactt cgagttggca
      541 gagggccaag cgatggtcgt agcccgccgc gcgctcgtcc ggggtcatcg gccacggcag
      601 tcgctgcaac cagcggtcaa agaaggcctg gacgtcggca gggccgaggg cgtcgcaggc
      661 cgcctgtaag gcggcggggt cggcgcagga gagaaaccca ttgtcgaggc tcgcaaaggg
      721 aatgtgatcg cgccgcaggc gttgcttgac ccactcatgg ccattgagac agagcttcac
      781 gggatagggc atgtaggtgc cgatcttgag aaatgccggg ccccagtcct gatcgtggac
      841 gtagaagtaa tagtggttga cggcgacgga ctgccgcgcg aaatcaaaca cgggtgtccg
      901 gcctcggccg ggtcgctggt gcgctttgaa ggaccgcatc ttctcctgcg cgacgccgat
      961 gaacacgacg ccgtcgcggg cggtgaatcg cgagcggtct ttcgccaccc gctcgtcctt
     1021 gctctcgccg cgctcgaagg gcacgacctc cagccgctgc gtcgccgcga attgctggac
     1081 cgccgcgacg aagcggtcgt ggcgcggttt cagcagggcg ggcgacggca cgggatagcc
     1141 gagatagtcg tggaggaagt agcataggcc accggacgtc tggagcttgg gcatatacgc
     1201 gtgcagatac acgcggtcga tacatcggac ttcaagcgac acatgctgac ggataatcgt
     1261 gtggatgttg ggcgtcatcg gatcctcctt gccgggaagg tcgatgatgg tactccttcc
     1321 ggagcaccac gtccgtggac gacgcccgac atccttgctc gcacctggca cggcctgggc
     1381 tatgactcag gcgtgggcgc aacccgccat aactgccggc gaagctgcgc cggctaccac
     1441 gcgtcccttc gctcggcggt ttgaggcgca gcttcgctgg cagcccgtgg ttaaagggct
     1501 ccttctaagg caccgacggg taataactcg aaaccacgat tcggcgtcaa cgcagaccgg
     1561 tgatcggcgg aaggggtcgt atgaacgcgc aagagctcga cggcgcacgt ccacagcgcg
     1621 accacgagcg ccagtaccgc cgccagtcgc ccttgcagac cgcgcggcgt gccgagtccg
     1681 atgacgtgac gcatcatgag cccgagattg aacccaccgg catggatcaa cagccgcttc
     1741 agaatgttcg tatgaccgcg gagatgcgtc cggcgcatcc cgccggtgtc gtagacgtgc
     1801 gcgaacgcgc gttccacata cgcgccgcgt cgccgcatca gccggcgtcc gcgggggccg
     1861 cggatccgtc ggcgattgcc gtaaaccggg gctcgggcct ccggttcctg cgaccagtcc
     1921 cgacgaccgc gagccggctc cgccacatac gagcggacgc ccagtgcggc gagttcgacc
     1981 atgatccgat tgctgtgata ccccttgtcg gtgatgatct cttccagggg ctgcgggtcg
     2041 tcgacgccgg cctgcgccgc ttcgatctgc tcgacggcgg ccgtgaccgt agcgacgatc
     2101 gtcgtcgtgt cgccttggtc cgcgccctgc acggtcaccg cgacgatcgc gcccgtctcg
     2161 aggtccacgg cgtgctcggc cttgtgggcc agatgcgtcc ggccatcttt catcttcgtg
     2221 accttcgcat ccgagtcgtg cgggttcgtc cagtccttgt tcgacgtctt cttcttccgc
     2281 ttccgatcca gccgcgccaa gtcctcccgc gtcggggtct cgatgcccga cgcctgcgcg
     2341 agccgcgtca gaaactcctg ataggtttcc tcggtatccc gccgcacgat gctgcgcatc
     2401 gcggcgttgg cttccagcgt ggtcgcatcg acggccaccg tctttccgtt gagcagcccg
     2461 acttcgacca gccgctgctg cacccacgta aagacggcgc gatgcgtttc cacatcgatc
     2521 cggcggcgcg tccgcgaaat cgtcgagtga tccggggccg cctcctggag cgccaggcgc
     2581 acgaaactgc ggatcgccaa ggagtcggcg gcccgccacg cgatgccgcg ctccgcgtcg
     2641 atgccttcaa aatatccgac caacaacagc cgaaagtact gcccgggcgg cagactcggg
     2701 cgccccagga ccgcggcgta gaaccggcga cactggtctt ccacgaaccg gtcgaagccg
     2761 taggcgtcca agattgcgtt cacgcgtgtg tagaacggat gccccggcga cgccggcagg
     2821 tcagacgcgg cgatccacaa cggcgattga tccgggtctc gcgttcccat cgacatgcct
     2881 ggcagtgtag gaccgaccaa tcagttctgg ctaatgatcg aatcaataca ccggctcaca
     2941 atcggccgcg ggcagccctg cagactttaa ccacgggctg ctagagcctg gtcatgcgcc
     3001 tcgtgttcgt ttcagccggt cgcggcgtcc tgcggtcgga tgtatgatcg cggtcgtgtg
     3061 cggcggtcag cacaaccatt aatcacagcg tgacgggagg attgcggtgg ctgaagtgag
     3121 tgtcaacaac ggcgtcaatg tcggagcact gctggcggcg cgggaagcac tgaaaggtgc
     3181 gccgcaggca gccaagttca cgtggcgcgc gtcctgcaaa tggcagaacg gcacgcacag
     3241 cgtgtcgaag gtcgaaggct tccatgggct cggcgaggag cagaagcaca agaccgagtt
     3301 ctcgttcgaa gccgatcatc ccgaggtctt tgcctccgag gatctcggcg cgacgccggt
     3361 cgagtacgtc ctcgtcggcc tggcgagctg tctgacggca ggcgtcgcct cggtcgcgca
     3421 gaatcgcggc gtccagctcc gctcggtcga agccaggctc gaagggtcga tggacatcca
     3481 ggggattctc ggcatcgaca gcgacgtccg caacggctac gacgacatca aggtcacctt
     3541 caatatcgac gccgacgcgt ccaagaagga gatcgaggcg atcgtggcgc agtcgcagaa
     3601 gcgttcggcg gtgttcgaca tcatcgccaa cccgaccacc gtcagcgtgg tagtcgccta
     3661 gtggcccgca caggatgatc aagaccacga cgaacgacga tcaccgccgc cgccgcgccg
     3721 aaactcg
//
LOCUS       DASYLX010000060         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1429376,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000060 DASYLX010000000
VERSION     DASYLX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429376"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGP27_01605"
     CDS             <1..541
                     /locus_tag="VGP27_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560627.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV7850044.1"
                     /translation="LGARWPVYDEALRQTWVGAEMQMLREAADAGVPTLGVCFGGQLL
                     AQAFGGTVARSPKPEIGWYEVASDDEDLIPGGSWFEWHFDTWTLPPGATEIARTANAS
                     QAFVLGRALGLQFHPEVDHALLDAWLSDDRNGEVASVGLTHDELRSATKHFENDAARR
                     IRALVRGFLSRVARQPCPS"
     gene            complement(523..>1672)
                     /locus_tag="VGP27_01610"
     CDS             complement(523..>1672)
                     /locus_tag="VGP27_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV7850045.1"
                     /translation="FDPPHRFVDVLSSAGPRSLPPRVIGWWRHTHEFSEAPGGGTTVR
                     DEVDTTVPAAALRPTFVYRHRQLADDLAAHRDATAAGAKPMVVAVTGASGLIGSALTA
                     LLTAGGHRVVRLVRRAARVKDERQWRPEAPAADLLSGVDAVIHLAGASIAGRFTNGHK
                     GAIRDSRIEPTRRLAQAAADADDGPQVFVSASAIGIYGYDRGDALLCEDSVRGGGFLA
                     DVVADWEAATTPADDAGIRVVTVRTGIVQAAAGGTLRLFRPLFLAGLGGRLGSGEQWL
                     SWIGIDDLLDVYYRAVYDARLVGPVNAVGPTPVRNADYTKTLAGVLHRPALVPVPSFG
                     PRLLLGGQGARELAEADQRVVPTKLTELGHRFRHATVEDALAHQLGHG"
BASE COUNT          253 a          599 c          591 g          229 t
ORIGIN      
        1 gctcggcgca cggtggccgg tgtacgacga ggcactgcgc cagacctggg tgggcgcaga
       61 gatgcagatg ctgcgcgagg ccgcggatgc cggagtgccc acgctcggcg tgtgcttcgg
      121 cggtcagttg ctcgcccagg ctttcggcgg gacggtggcc cgatcgccga agcccgagat
      181 cggctggtac gaggtggcat ccgacgacga ggacctgata cccggcggct cctggttcga
      241 atggcatttc gacacctgga cgctgccccc tggcgccacc gagatcgcga gaaccgcgaa
      301 cgcatcacag gccttcgtgc tcggcagagc cctcgggctg cagttccacc ccgaagtgga
      361 tcacgcgctg ctggacgcct ggctttccga cgatcgcaac ggcgaggtgg ccagcgtcgg
      421 tctcacccat gacgagttac gttcggcgac aaagcatttc gagaacgacg cggcccgccg
      481 catccgcgcc ctggtgcgcg gcttcctatc gcgggtcgcc cgtcagccgt gtcccagctg
      541 atgggctaac gcatcctcga cggtggcgtg ccgaaaccgg tgccccaact cggtgagctt
      601 cgtcggcacc acccgctggt cggcctcggc cagctcgcgg gcgccctgac cgcccaacaa
      661 caggcggggc ccgaacgacg gcacaggcac cagggcgggc cggtgcagca cacccgccag
      721 cgttttggtg tagtcggcgt tgcgcacggg cgtcggtccg acggcgttga cgggaccgac
      781 cagccgggcg tcgtaaacgg cgcggtagta gacgtcgagc aggtcgtcga taccgatcca
      841 ggacaaccat tgctcgccgc tgcccagacg gccgccgaga ccggccagga aaagcggtct
      901 gaacagccga agggtgccgc ccgccgcggc ctgcacgatc ccggtgcgca cggtgaccac
      961 ccgaatgccg gcgtcgtcag cgggagttgt cgccgcctcc cagtccgcca ccacgtctgc
     1021 caggaagccg ccgccgcgca cgctgtcctc gcacagcagc gcgtcaccgc ggtcgtatcc
     1081 gtagatgccg atcgccgagg cgctgacgaa cacctgcggc ccgtcgtcgg cgtcggccgc
     1141 cgcctgcgcc agcctgcgcg tcggctcgat gcggctgtcg cgaatcgcgc ccttgtggcc
     1201 gttggtgaat cgacccgcta tcgaggcgcc cgccagatgg atcacggcgt cgacgcccga
     1261 caacaggtcg gctgcagggg cttcgggccg ccactgccgc tcgtccttga cgcgcgcggc
     1321 ccggcgcacc agccgaacaa cccggtgccc accggcggta agcagcgccg tcagggccga
     1381 tccgatcagc ccggacgcgc cggtgacggc gacgaccatc ggcttggcgc ccgcggcggt
     1441 ggcgtcgcgg tgggcggcga ggtcgtcggc cagctggcga tgccggtaga cgaaggtcgg
     1501 ccgcagcgcc gcagcgggca ccgtggtgtc gacctcgtcg cgcaccgtgg tgccgccccc
     1561 cggtgcctcc gagaactcgt gggtgtgccg ccaccagccg atgacacgcg gcggcagcga
     1621 tcgcggtccg gccgaggaca gcacgtcgac gaaccggtgc ggcggatcga aa
//
LOCUS       DASYLX010000061         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_113049,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000061 DASYLX010000000
VERSION     DASYLX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113049"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGP27_01615"
     CDS             <1..258
                     /locus_tag="VGP27_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850046.1"
                     /translation="LAVLHLEDEPVVRARSVSAAKATVGRAARFIGQQAVQLHGGMGM
                     TEELAIGHYFKRLTAMQYEFGTTDHHIGRYAELSRLAPLVV"
     gene            264..1160
                     /locus_tag="VGP27_01620"
     CDS             264..1160
                     /locus_tag="VGP27_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7850047.1"
                     /translation="MTSRGWLLFVAMSIIWGIPYLMIKVAVEGVSVPVLVFARTAVGA
                     VVLLPLVLKTGIPAMIREHWKALLGFSFFEIIAAWFLISDAERHLTSSMTGLLIAAAP
                     IIAAILDRFTGGEQHLGFRRVLGLGIGLAGVAVLAGPHLVGGSAWPIIEVLMVATCYA
                     IAPLIAARHLRDVPALPMTATCLGFGALVYAAPAAATWPTEMPSLRVLSALAGLAIIC
                     TALAFIVFFALIREVGAARALVFTYVNPAVALLAGVIVLNEPLTAWNVAALLLILFGC
                     VLATHRPGEAVPDPQRDEHVAR"
     gene            1218..2147
                     /locus_tag="VGP27_01625"
     CDS             1218..2147
                     /locus_tag="VGP27_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7850048.1"
                     /translation="MKTTGPVDARQLDGSAPVDADIRRAMLRHMLMARGIDDEAGRLE
                     NQGALDLWLSCRGQEAAQVGSAMAIGGEAVIFPSYREHAVAVVRGIDAVSLAAQWAGR
                     TFCGWNPRRHRFFPYTLVLAAQALHAVGYAIGRRLQNTAETVVVYVGDGATSEGDMSE
                     ALNLAAVESAAVLFICQNNGWAISKPSHEQMLTSIAHRANGFGIRSWSISADDPERTY
                     LSCLEAMQHVESSRSPGLIELRTTRIGGHTTSDPHTSYREAEELAAAEASDPVARYRT
                     TLLTSGVVDEEWLGTVEAEVDKTRRALLEAFVL"
     gene            2144..3127
                     /locus_tag="VGP27_01630"
     CDS             2144..3127
                     /locus_tag="VGP27_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7850049.1"
                     /translation="MTGTTTSLSLADSLNGALHELMETDESIVLLGQDIGRLGGVFRV
                     TKGLQERFGADRVRDAILAESSIVGQAIGMSLTGLKPICEIQFEGFIYPAMNQIVTQA
                     ARMTTRWNDDITLNLVIRVPVGGGIRAVEHHSESNEAYFAHTPGLHVAFPSCPTDAAA
                     MLKYSSSVGSPVIFFEPKRLYWKRRRQPRELSEPASPLSARIVRRGSDITVAGFGAVL
                     EEVLAAAEKVAPGIAVEVVDLRWIAPMDIDTVLESVARTGRLLVVHEAVGFGGVGAEV
                     VATVAEHGTSHLKSPMRRLAAPRRLYPPADYEKHYLIGVSDIVGAIEEMCV"
BASE COUNT          504 a         1028 c         1039 g          560 t
ORIGIN      
        1 ctcgccgtac tgcacctcga agacgagcct gtcgttcgtg cgcggtcggt gtcggcagcg
       61 aaggcgacgg tcggcagagc ggcccggttc atcggccagc aggctgtcca actgcacggc
      121 gggatgggca tgaccgagga actggcgatc ggccactact tcaagcggct gacggcgatg
      181 cagtacgaat tcggcaccac cgaccaccac atcggacggt atgcggagct gtcgcggctg
      241 gcgcctctcg tagtgtgatc gacgtgacct cgcggggatg gctgttgttc gtcgcgatga
      301 gcatcatctg gggcattccg tacctgatga tcaaggtcgc cgtggaaggc gtctcggtcc
      361 ccgtgctggt attcgcgagg actgccgtcg gtgcggtcgt actgctcccg cttgtgttga
      421 agaccggaat tcccgcgatg atccgcgaac actggaaggc cctgctgggc ttctccttct
      481 tcgagatcat cgccgcgtgg ttcctgatct ccgacgctga acgccatctg accagttcga
      541 tgaccggcct gctgatcgcc gccgcgccga tcatcgcggc gatcctcgac cgcttcaccg
      601 gcggtgagca acacctcggg ttcaggcggg tgctgggcct cggcatcgga ctcgccgggg
      661 tggccgtgct cgccgggccg catctcgtgg gcggcagtgc ctggccgatc atcgaggtgc
      721 tgatggtggc cacctgctac gcgatcgccc cgttgatcgc cgcccggcat cttcgcgatg
      781 tgcccgcgct tccgatgacc gcaacgtgcc tcgggttcgg cgccctggtg tacgccgctc
      841 ccgccgcggc cacatggccg accgagatgc cctcccttcg agtgctgtcg gcactggccg
      901 ggctggcgat catctgcacg gccctcgcct tcatcgtgtt cttcgcgctc atccgcgaag
      961 tcggagccgc acgcgcactc gtcttcacct acgtgaaccc cgcggtcgcg ctgctggccg
     1021 gcgtgatcgt cctgaacgag ccgctcaccg cgtggaacgt ggcggcgctg ttgctgatcc
     1081 ttttcggctg cgtactggcg acccatcggc ccggcgaggc ggtgccagac ccgcagcgtg
     1141 atgagcatgt cgccaggtga ccgccaccac cagggcaaat accggtggaa aggccctgtt
     1201 gccattagct tggttatgtg aagacaaccg gacccgtcga tgcgcgtcag ctggacggca
     1261 gcgcgcccgt cgacgcagat atccgacggg cgatgctgcg ccacatgctg atggcccgcg
     1321 gcatcgacga cgaggcgggg cggctggaga accagggggc gctcgacctg tggctgtcgt
     1381 gccggggtca ggaagcggcg caggtcgggt ctgcgatggc gatcggtggt gaagcggtca
     1441 tctttcccag ctatcgggag cacgcggtcg cggtggtgcg cggcatcgat gctgtctcgt
     1501 tggccgcgca gtgggcaggc cgcacgttct gcggctggaa tccgcgcagg caccggttct
     1561 ttccctacac cctggtgctg gcggcacagg cactgcacgc cgtcgggtac gccatcggtc
     1621 ggcgcctgca gaacacggcc gaaaccgtgg tcgtctatgt cggcgacggt gccaccagtg
     1681 aaggcgacat gtccgaggcg ttgaacctgg ccgcagtcga gtccgcggcg gtgctgttca
     1741 tctgccagaa caacgggtgg gcgatctcca aaccgagcca tgagcagatg ctgacctcga
     1801 ttgcccatcg cgccaacgga ttcggcattc gatcgtggtc gatctcggcc gacgatcccg
     1861 agcgcaccta cctgagttgc ctcgaggcga tgcagcacgt cgagtcgtcc cgatcgccgg
     1921 gcctcatcga gctgcggacc acccgcatcg gagggcacac cacctccgac ccccacacca
     1981 gctaccggga agccgaagaa ctcgccgcgg cggaggcgag cgatccggtt gcccgctacc
     2041 gcacaacgct gctcacttcc ggcgtcgtcg acgaggaatg gctcggcacc gtcgaagccg
     2101 aagtcgacaa gactcgtcgg gcactgctgg aagccttcgt cctatgaccg gtacgacgac
     2161 ttcactcagc ttggccgact cgctcaacgg cgccctgcat gaactgatgg agaccgacga
     2221 gtcgatcgtg ctactcggtc aggacatcgg ccgcctcggt ggcgtgttcc gggtgacgaa
     2281 gggccttcag gaacgcttcg gagcggatcg tgtccgcgac gcgatcctcg cggaatcctc
     2341 catcgtggga caggccatcg gcatgtcgct gacggggctc aaacccatct gcgagatcca
     2401 gttcgagggt ttcatctatc ccgccatgaa ccagatcgtc acccaggccg cgaggatgac
     2461 gacacgctgg aacgacgaca tcacgctcaa cctggtgatc cgggtacccg tcggcggggg
     2521 catccgcgcc gtcgagcatc acagcgagtc caacgaagcg tacttcgcgc acacaccggg
     2581 tctgcatgtc gcctttccgt cctgcccgac cgacgccgcc gcgatgctga agtactcgag
     2641 ttcggtgggc tcaccggtca tcttcttcga gccgaagcgc ctgtactgga agagaaggcg
     2701 gcaaccgcgc gagctttccg agcctgcgtc gccattgagc gcacgcatcg tgcgacgagg
     2761 ttccgacatc accgtcgcag gattcggcgc ggtgctcgag gaagtgcttg cggccgccga
     2821 aaaggtcgct cccgggatcg ctgtcgaggt cgtggatctg cgctggatcg cgccgatgga
     2881 catcgacacc gtgctcgagt cggtcgcccg caccggtcgc ctgctggtgg tacacgaggc
     2941 ggtcgggttt ggtggcgtcg gcgccgaggt ggtcgcgacg gtagccgaac acggcacttc
     3001 gcacttgaag tcgccgatgc ggcggttggc cgcgccacgg cggctctacc cgcccgccga
     3061 ctacgaaaag cattacctga tcggcgtatc cgacatcgtc ggcgcgatcg aggagatgtg
     3121 cgtgtgagcg a
//
LOCUS       DASYLX010000062         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_661495,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000062 DASYLX010000000
VERSION     DASYLX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661495"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGP27_01635"
     CDS             <1..318
                     /locus_tag="VGP27_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEV7850050.1"
                     /translation="EFMDTVRTVERAIAADRRGEDVPEELDIASLGVITEEEWRAFWP
                     SAESEPAPDRIPEPTPADVPEPGPVEVPEPAPVGVPEPTPEDVGTNGVEVEVDEEAVE
                     PRS"
     gene            complement(384..1646)
                     /locus_tag="VGP27_01640"
     CDS             complement(384..1646)
                     /locus_tag="VGP27_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEV7850051.1"
                     /translation="MTERPPRRGLSRRGFVTGALGTGVAVGAGAALAGCSSPEPAPPR
                     LPQSEFVPFEGRHQAGITTLPIPEQGLIASFNLHATDRAGLKATLQELTEEIRGLMAG
                     KPPEIRDRAYPPVDSGILGEKPPPDNLSVLVGLGASVFDDRFGLADRKPKDLETMPFL
                     ANDRLDPKLSHGDISIILEAGHNDTVQFALRQLMRRTRSELTLRWMIDGYARGIGAGK
                     SSEALTPRNLLGFKDGTANLDVDDDTLMNQHVWVQPGDGEPEWAVGGSYQAVRIIRMF
                     VEFWDRTRLAEQEALIGRKKVSGAPLGMTDEFADPDYAEDPDGKRVPLDAHIRLANPR
                     TTESQQNLILRRGFNYSRGFDGAGRLDQGLAFVAYQRSLQQGFLAVQSRLKGEPLEEY
                     ILPVGGGFFFVLPGLTGSDRFLGDQLVD"
     gene            complement(1643..2572)
                     /locus_tag="VGP27_01645"
     CDS             complement(1643..2572)
                     /locus_tag="VGP27_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HEV7850052.1"
                     /translation="MKLHLTWPVSIATAALVLAGCSSSDAGSTDESSESSSPGETSTS
                     AGAATSPALQKAADDYKAYAVAQSDELVKVVKVFTDAVRANNLKAAQEAFAPSRQPWE
                     RIEPIAELVENTDAKTDSRVDDFASVDDPAFTGWHRLEYLLFEKNTTEGGAPFADQLD
                     ADIANLQEELPKVDVKPINVANGAAELIEEVSQGKLTGEEDRYSKTDLWDFDANLQGS
                     QVAVDKLSGPLKDADAELLGKISAGFSQIFDTMRPLRQGDGWANFCPENSEFPSPRCP
                     SVTVNPQVADALKAQLAGLSENLSQVAGALKLQ"
     gene            2744..>3231
                     /locus_tag="VGP27_01650"
     CDS             2744..>3231
                     /locus_tag="VGP27_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850053.1"
                     /translation="MSPVLWLRAVAVIGATALLMAASCSWQLGTPIPEGVPPPTGDPV
                     PQIDTHAKGRPADQLHSWAAERASALGIPVEALEAYAYAARVAEVENPDCHLAWPTLA
                     GIGMVESHNGTYRGATIAPNGDVAPLIRGVRLDGTMGNLQIIDSDSGLLDGDPGIDRA
                     MGP"
BASE COUNT          507 a         1033 c         1123 g          568 t
ORIGIN      
        1 gagttcatgg acacggtgcg cacagtggag agggcgatcg ccgcagaccg tcgcggcgag
       61 gacgttcccg aggagctcga catcgcctcg ctcggggtca tcaccgagga ggagtggcgg
      121 gcgttctggc catccgccga atccgagcct gcgccggacc gcattcccga gccgacaccc
      181 gccgacgtgc cggaacccgg gccagtcgag gtgccggagc ccgcacccgt cggtgtaccg
      241 gagccgacgc ccgaggacgt cggcacgaac ggtgtcgagg tcgaggtcga cgaggaagcc
      301 gtcgaaccgc gctcgtagaa ccgatgtggc ggtcgagtct gcggccacgg cgggtttcgt
      361 cggaattcgc gcactgcagg cagtcagtcg acgagttgat cgccgaggaa ccggtctgaa
      421 cccgtaagcc ccggcagcac aaagaagaac ccgccgccga cgggcaggat gtactcctcg
      481 agcggctcgc ccttcagccg gctctgcacg gcgaggaagc cctgctgcag gctgcgttgg
      541 taggcaacga atgccaggcc ctggtcgagc ctgccggcgc cgtcgaatcc gcgcgagtag
      601 ttgaaacccc ttcgcaggat gaggttctgc tggctctcgg tggtgcgcgg gttcgccagt
      661 cggatgtggg cgtccaacgg gacccgcttg ccgtcgggat cctcggcgta gtcgggatcg
      721 gcgaactcgt cggtcatgcc aaggggggcg ccgctgacct tcttgcggcc gatgagcgct
      781 tcctgctctg cgaggcgggt acggtcccag aattcgacga acatccggat gatgcggacg
      841 gcctgatacg agccgccgac cgcccattcg ggttcgccgt ctcccggttg cacccatacg
      901 tgctggttca tcagcgtgtc gtcgtcgacg tcgaggttcg cggttccgtc cttgaatccc
      961 aacaagtttc gcggagtgag ggcctccgag gacttgcccg cgccgattcc ccgggcgtac
     1021 ccgtcgatca tccaccgcag tgtcagctcg cttcgggtgc ggcgcatcaa ctgccgcagc
     1081 gcgaactgca cggtgtcgtt gtgccccgcc tcgaggatga tcgagatgtc tccgtgggac
     1141 agtttcgggt cgagtcgatc gttggcaagg aacggcatgg tttccaggtc cttgggcttg
     1201 cgatccgcca ggccgaaccg gtcgtcgaac accgaggcgc cgaggccgac gaggaccgac
     1261 agattgtcgg ggggtggctt ctcgccgagg atgccggaat cgactggggg ataagcgcga
     1321 tcgcggatct cggggggctt gcccgccatc aggccgcgga tctcctcggt gagttcctgc
     1381 agcgtcgcct tcaagcctgc gcggtcggtg gcgtgcaggt tgaacgaggc aatcagaccc
     1441 tgttcgggga tcggcagcgt ggtgatgcct gcttgatgcc ggccctcgaa cgggacgaac
     1501 tcggactgcg ggagccgcgg cggtgccggc tcgggcgacg aacacccggc cagcgccgcg
     1561 cccgcaccca ccgcgacacc ggtgcccaac gccccggtga cgaacccgcg gcgggatagt
     1621 cctcgtcgtg gtggccgctc ggtcactgca gcttcaaggc tcctgcaact tgggacaggt
     1681 tttccgacag accggcgagc tgggccttca gcgcgtcggc cacctgtggg ttgacggtca
     1741 ccgaggggca tcgcggcgac gggaactcac tgttctcggg gcagaagttc gcccaaccgt
     1801 cgccctgccg cagcggccgc atggtgtcga agatctgcga gaagcccgcg ctgatcttgc
     1861 cgagcagctc ggcgtcggcg tccttcagcg gcccgctcag cttgtcgacg gccacctgcg
     1921 aaccctgcag gttggcgtcg aagtcccaga ggtcggtctt ggagtaccgg tcctcttcac
     1981 cggtcagctt gccctgcgat acctcttcga tgagctcggc ggcgccgttg gcgacgttga
     2041 tgggcttgac atcgaccttc ggcagttcct cttgcaggtt ggcgatgtcg gcgtcgagtt
     2101 ggtcggcgaa cggagcaccg ccctcggtgg tgttcttctc gaaaagcaag tattcgaggc
     2161 ggtgccaccc cgtgaaggcc gggtcgtcga cgctggcgaa gtcgtcgacg cgggagtcgg
     2221 tcttggcgtc ggtgttctcg acgagttcgg cgatgggctc gatgcgctcc cacggctggc
     2281 gcgacggcgc gaaggcctcc tgggcggcct tcaggttgtt cgcgcgcacg gcgtcggtga
     2341 acaccttcac gaccttgacg agctcgtcgg actgggccac ggcgtacgcc ttgtagtcgt
     2401 ctgcggcctt ctgaagcgct gggctggtgg cggccccagc agacgtcgac gtctcgccgg
     2461 gcgaactcga ttcgctgctc tcatcggtgc tgcctgcgtc gctgctggag caaccggcta
     2521 gtaccaaggc cgctgtcgca atcgacacag gccaggtgag atgcagtttc attgagcctc
     2581 ctcgtagtcg gacgcgtgaa cattacaacg ttttgctcaa cgagggtagg cacacctaaa
     2641 tattgtttgc ctacccttag cggcgctcgg caacggcggg tcccggggag cactctgcga
     2701 gccatgggac gatggatttc aacaaagggt tttgaggagt ccggtgtcgc cagtgctttg
     2761 gctgcgggct gtcgcggtga tcggcgcgac agcgctgctg atggccgcaa gctgctcatg
     2821 gcagctcggc acaccgattc ccgaaggcgt cccgccgccg acgggtgacc cggtgccaca
     2881 gatcgacacc cacgccaagg gcaggccggc cgaccagctg cactcctggg ctgccgaacg
     2941 ggcgtcggcg ctgggcatac cggtcgaggc gctggaggca tacgcctacg cggcccgggt
     3001 cgccgaggtg gagaaccccg attgccatct ggcctggccc acactcgcgg gaatcggcat
     3061 ggtggagagt cacaacggca cctatcgcgg cgcgaccatc gcgccgaacg gtgatgtcgc
     3121 gccgctgatc cgcggcgtgc ggctggacgg cacgatgggc aatctgcaga tcatcgacag
     3181 cgactctggt ctgctcgacg gcgatcccgg tatcgaccgg gcgatgggcc c
//
LOCUS       DASYLX010000063         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_771503,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000063 DASYLX010000000
VERSION     DASYLX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771503"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /locus_tag="VGP27_01655"
     CDS             complement(<1..>1601)
                     /locus_tag="VGP27_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7850054.1"
                     /translation="QLGTEPMHPVLMDLCTGVAFPAFPALLAAEQGVNDLFLPLRYGQ
                     VMLREKMPRRFYCRARWHTSELDSETQVFDLDFVDRDGRHLGGIREFTVKRAPREALL
                     RGLGGDATRLLYTLGWHEVPPPASNDETASVNGTWLIAGFDELAANVPGCIPFDAITD
                     PEPLGQLLAQAHERGLPFAGVVWRASAPGAHGGGAGPENWAARLEAEIANLLSAVHTV
                     QGGEVKLPGGLWIVTEQAVATESGEPVDPVQAALWGFGRTTINEEPALRCKLVDCDGS
                     AQAVQALANLLASPVDEPELALRQGKLLASRLLPWARSGHLTVPRSTDYVLAPTERGA
                     IDNLRLTETDVPTPDEGYVQVRVNAAGLNFRDVLNVLGLYPGDPGPIGGDFAGIVTQL
                     GSGVTGIEVGQRVYGFMQGAFSSRFNVPVQLVAPVPDGIGAVAAATIPAAALTARLAF
                     DWAQLQPGDRVLIHAASGGVGLAAIQMAQQHGAIVFATASTYKRATLRKMGVKYVYDS
                     RTTEFADQILADTDGAGVDVVLNSLT"
BASE COUNT          252 a          556 c          532 g          261 t
ORIGIN      
        1 gtcaggctgt tgagcaccac gtcgacgccc gcgccgtcgg tgtcggcgag gatctggtcg
       61 gcgaactctg ttgtgcgcga gtcgtagaca tatttcacac ccattttgcg cagcgtcgcg
      121 cgcttgtagg tgctcgcagt cgcgaagacg atggccccgt gctgctgtgc catctggatt
      181 gccgcaagcc cgacgccgcc gctggcggcg tggatgagca cgcgatcacc gggctgcaac
      241 tgcgcccagt cgaatgcgag tcgggccgtg agagcggcag cggggatggt ggccgccgcc
      301 accgcgccta ttccgtcggg caccggcgcc accaactgga ccggcacatt gaagcggctg
      361 gaaaacgcgc cctgcatgaa gccgtagacg cgctggccga cctcgatccc ggtgacgccg
      421 ctgcccaact gcgtgacgat gccggcgaag tccccgccga tcggcccggg gtcgcccggg
      481 tagagaccga gcacgttgag cacatcccgg aagttgagac ccgcggcgtt tacccggacc
      541 tgcacgtagc cctcgtccgg cgtcggcacg tccgtctccg tcagacgcag attgtcgatc
      601 gccccgcgtt cggtgggcgc cagcacgtag tcggtcgagc gcgggaccgt cagatgaccg
      661 ctgcgcgccc atggcaacaa ccgcgatgcc agcaactttc cttgccgcag tgcgagttcc
      721 ggctcatcga ccggtgatgc cagcagattg gccagcgcct gcacagcctg cgctgatccg
      781 tcgcagtcga ccagtttgca gcgcagtgcc ggttcctcgt tgatcgtggt gcgcccgaaa
      841 ccccacagcg ccgcctgcac cgggtcgacc ggttcgcccg actcggtggc cacggcctgc
      901 tcggtgacga tccacaggcc gccgggcagc ttcacctcgc cgccttgcac ggtatgcacg
      961 gcgctgagca ggttggcgat ctcggcctcg agccgcgcgg cccagttttc cgggccggcg
     1021 cccccaccat gtgcgccggg tgcactggcg cgccagacga cgcctgcaaa cggcagaccg
     1081 cgctcgtgag cctgtgccag cagctgtccc aacggctccg gatcggtgat cgcgtcgaac
     1141 gggatgcagc caggcacgtt ggcggccagt tcgtcgaatc cggcaatcag ccaggtgccg
     1201 ttcacacttg cggtctcgtc attcgacgcc ggtggcggca cctcgtgcca gcccagggtg
     1261 tacagcagcc tggtggcgtc gccgccgagg ccccgcagca acgcttcgcg cggtgcgcgt
     1321 ttgaccgtga actcgcggat cccgccgagg tggcggccat cccgatcgac gaaatcgagg
     1381 tcgaagacct gggtctcgct gtcgagttcg ctggtgtgcc atctcgcgcg gcagtagaac
     1441 cgccgaggca tcttctcccg cagcatcact tgcccgtacc gcaacggcaa gaacagatcg
     1501 ttgacgccct gttcggccgc gagaagtgcc ggaaacgcag ggaaggcgac gccggtgcac
     1561 aggtccatca gcaccgggtg catcggctcc gtgccgagtt g
//
LOCUS       DASYLX010000064         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2416956,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000064 DASYLX010000000
VERSION     DASYLX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416956"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGP27_01660"
     CDS             complement(<1..606)
                     /locus_tag="VGP27_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850055.1"
                     /translation="MTAPLAVRGTAIQHRFGDTLAVDGVDLEVAAGAVFGLLGPNGAG
                     KTTTLRMITTLLPASIGTIEVFGVDVARHKLAVRRLIGYVPQQLSADGSLTGRQNVAL
                     FARLFDVPRARRRQVVDDSLESVGLADVADRTAGTYSGGMIRRLELAQALVNAPRLLV
                     LDEPTIGLDPIGRASVWDHIADVRASTGMTVLVTTHYMEEAD"
     gene            complement(603..1064)
                     /locus_tag="VGP27_01665"
     CDS             complement(603..1064)
                     /locus_tag="VGP27_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV7850056.1"
                     /translation="MTSPDSAVLGDELMATTAALRRVVRRQLRSAVSGPPLRGAQVEL
                     LQVVERQPGIGIARAGCVLHLAANSVSTLVNQLIDIGMLVRQTAPDDRRVAQLWLTEA
                     ATQRLAAWRQARADFVGGGMAALSVADHQALARALPALRALATALDGGEER"
     gene            complement(1318..1535)
                     /locus_tag="VGP27_01670"
                     /pseudo
     CDS             complement(1318..1535)
                     /locus_tag="VGP27_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725525.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          276 a          532 c          483 g          244 t
ORIGIN      
        1 gtcggcctcc tccatgtagt gcgtggtgac cagcacggtc atcccggtgc tcgcgcggac
       61 gtcggcgatg tggtcccaca cgctggcccg gccgatcggg tccaggccga tcgtcggctc
      121 gtcgagtacc agcagtcgtg gcgcgttgac cagcgcctgg gccagttcca gccggcggat
      181 catgccgccg gagtaggtac ccgcggtgcg atcggcgacg tcggccaaac cgacagactc
      241 cagcgagtcg tccacgacct ggcgccgccg cgcgcgcggt acgtcgaaca aacgcgcgaa
      301 gagcgctacg ttctgccggc cggtcagcga gccgtcggcg gacagctgct gcggcacata
      361 cccgatcaac cgtcgcaccg caagcttgtg gcgagccaca tccacgccga aaacttcgat
      421 cgtcccgatc gacgccggca gcaacgtcgt gatcatgcgc agcgtggtgg tcttgccggc
      481 accgttgggc cccaacagcc caaacaccgc cccggcagcg acctccagat caaccccgtc
      541 caccgcgagg gtgtccccga aacgatgctg gatcgcggta ccccgcacag cgagcggagc
      601 agtcatcgtt cctccccacc atcgagagcg gtcgccaacg cgcgcagcgc aggcaacgct
      661 cgggccagag cctgatgatc ggcgacggaa agcgctgcca taccaccgcc gacgaaatcg
      721 gcgcgggcct gccgccacgc cgctaaccgt tgcgtggccg cttcggtcaa ccacaactgg
      781 gcgacacgac gatcatcagg cgccgtctga cggaccagca tcccgatgtc gatgagctgg
      841 ttgaccaacg tgctgaccga gttcgccgcc agatgcagga cgcatcctgc tcgtgcgatc
      901 ccgatacccg gctgccgctc cacaacctga agaagctcca cctgcgcacc tcgcaacggc
      961 ggccccgaca ccgccgagcg caactgccga cgcaccacac gccgcagagc agccgtcgtg
     1021 gccatcagct cgtcgcccag caccgcgcta tcagggcttg tcacacaacg acagtagctc
     1081 tcaataagac ctactcgcaa tagaagctaa cgttgatgat gctcgtagtc gacggcctac
     1141 tcccagtcgc cagcgatgga cctcggccag cgtgagcagt aacgctgacc agccacgggc
     1201 aactcgcggt aggactgtcg gccggcggct cggtggtggc ggctggctga tcgtttcccg
     1261 gtcacgtccc gcggcatggg tagatcgggt tcaccgtggc ggtcagcgta gatcgtcagt
     1321 tcgtcgtttg atttctttgt tcagccgctc cagggggttg gtgggccaga tctttttcca
     1381 gtgggcgacg gggaaggcgg cgaaggcagc agttcgggct cggcgtcgcg cagcatggtc
     1441 tcgaccttgc tgaactggcg gccgagcatg ccggcgatcg tcgtcgacca gttaaggatt
     1501 cggtcaccgg caaccacggc cgttgtcgta gcctg
//
LOCUS       DASYLX010000065         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_332475,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000065 DASYLX010000000
VERSION     DASYLX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332475"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VGP27_01675"
     CDS             complement(<1..759)
                     /locus_tag="VGP27_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEV7850057.1"
                     /translation="MPGWRTGAGHRLLALVATAGVLVLASDLRITHAHDAPGLVGGPF
                     SLLLNASDDLGPSHRDDAQLTVELRDANQPDRLVGWARRHSLSVRWQPGKNWAVVEGG
                     ADKVGSAFGVEVHDYRGQRGQLFYASPQQPSVPEQLRPEVVELGRILSYIPHHTSKPP
                     MLPTDVPDQGLQPSALLNTYNVSKLASTGFTGKGQTIVIFAFDGFDQADLDSFATLYK
                     LPRFKPTVVGGQPAEPRGETTMDLEVAHAIAPDAK"
     gene            complement(769..1035)
                     /locus_tag="VGP27_01680"
     CDS             complement(769..1035)
                     /locus_tag="VGP27_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850058.1"
                     /translation="MTTRRSTRRIPAIAGAAAIIALVGLSAACAKEEQKAPETSTTTT
                     TTTTTTTAPPQPTEKAPRLDPNGPNPFSPSVLAPPAPTAPPGDN"
     gene            1282..1965
                     /locus_tag="VGP27_01685"
     CDS             1282..1965
                     /locus_tag="VGP27_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850059.1"
                     /translation="MSLDRHRFDRAEQVEHLRRKMAAISGKVGPGRRGELPSNDVLPE
                     PESLLSPPESQTDALRTTLPRGTVAVLSGARSLSLSMVAAVTAAGGHAAIVGQPDVGL
                     LAAVEMGADLSRLAVIPEPGADPVEVAAVLMDGMDLVLLGLGGRSVPATRARAVVARA
                     RQKGCTLLVTGGEWQGASARLEARVSGYEVTGGSGERPISGCGRISRVRLAMRARGRA
                     FGSVHAVGE"
     gene            1962..>3360
                     /locus_tag="VGP27_01690"
     CDS             1962..>3360
                     /locus_tag="VGP27_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEV7850060.1"
                     /translation="MSTSRVLAIWCMDWPAVAAAAAADLPPTSPVAVTLANRVIACSA
                     SARAAGVRRGLRRRESQARCPELHVVTADPARDARHFENVTTAVDDLVPRAEVLRPGL
                     LVLPVRGAARYFGSEQTAAERLVDAVAAAGTECQVGIADRLPTAVFAARAGRIIEPGE
                     DAVFLSALSIRQLATEPSLAAAGREDLADLLWRMGIRNIGQFAALSRADVASRFGADA
                     IAAHRFARAEAGRGPSGREPSPDLDAVMDCDPPVDRVDAAAFAGRSLAGDLHRSLEAA
                     GVGCTRLAIHAVTANGEELNRVWRCAEPLTEDATADRVRWQLDGWLNRRNPNDRPTAP
                     ITVLRLQPVEVVSAEALQLPLWGGIGEEDRLRARRALVRVQGLLGPEAVQVPVLSGGR
                     GPAERITLTPLGDEPVPQADPAQPWPGQLPEPSPTVLLDDPVEVLDHEGNPVRVTNRG
                     LFSADPARLAAPGREG"
BASE COUNT          500 a         1053 c         1231 g          576 t
ORIGIN      
        1 cttggcgtcg ggtgcgatgg cgtgcgcgac ctcgaggtcc atcgtcgtct caccgcgcgg
       61 ttcggcaggc tggccgccga ccacggtggg cttgaacctc ggcaacttgt acagggtcgc
      121 gaagctgtcg aggtcggcct gatcgaagcc gtcgaacgcg aagatcacga tggtctggcc
      181 cttgccggtg aatcccgttg aggccagctt gctcacgttg taggtgttga gcagcgcgct
      241 cggctgcaaa ccctgatccg gaacgtctgt cggcagcatc ggcggtttgg acgtgtgatg
      301 cgggatgtag ctcaggatgc ggccgagttc gacaacctct ggacgcaact gctcgggcac
      361 cgacggctgc tgcggggacg catagaacag ctgaccacgc tggccgcgat agtcatgcac
      421 ctcgacaccg aatgcggagc ccaccttgtc ggcgccgccc tcgacgacgg cccagttctt
      481 gccgggctgc catcgcacgg acaggctgtg gcgacgcgcc cagccgacca accgatccgg
      541 ttgattcgcg tcgcgcagtt cgacggtcag ctgggcgtca tcgcggtgcg agggtccgag
      601 atcgtcagat gcgttgagca acaacgagaa cggtccgccg accagcccgg gcgcatcgtg
      661 cgcatgggtg atccgcagat ccgacgcaag tacaagcacc cccgctgtcg caaccaacgc
      721 cagcagtcgg tgcccggcgc ctgttcgcca accgggcacc gcggtgtact agttgtcgcc
      781 gggaggggcg gtgggagcgg gcggtgcgag gaccgagggc gagaacgggt tcggcccgtt
      841 ggggtcgagg cgcggggcct tctcggtcgg ctggggagga gccgtcgtcg tcgtcgtggt
      901 cgtggtcgtc gtggtcgagg tctcgggagc cttctgctcc tccttcgcgc atgccgcgga
      961 aagcccaacc agggcgatga ttgccgcggc tccggcgatg gccggaatgc gacgagtcga
     1021 acgacgtgtc gtcatcattt tcctatctgt cccccgactt gctgtggaac cgctgccagt
     1081 gcggttcgtc gttggaaacc ttatccgatg agcgaaccat tcccgaggtg attaagccgc
     1141 tggagaatcg gtcaaggaat cttcggcggc accgcgaacg cttgacgctt tcggtgggcc
     1201 ggtgtttact cgtcgtatcg aacatatttt cgaatgagcg atgattcaga tggggcagaa
     1261 ggtgctgttg tgaccgctgc gatgagcctt gatcggcacc ggtttgaccg tgctgagcag
     1321 gtagaacacc tgcgccgcaa gatggcggcg atatccggga aggtcgggcc cggacgacgt
     1381 ggcgagctgc cgtccaacga cgtgcttccc gagccggaaa gtttgctatc gccacccgaa
     1441 tcccagaccg atgcgcttag gaccacattg ccgaggggaa cggtggctgt gctgtcgggt
     1501 gcccggtctc tgtcgttgag catggttgcg gcggtgacgg cagcgggtgg gcatgcggcc
     1561 atcgtcggtc aacccgacgt tggtctgctt gcggccgtgg aaatgggagc ggatctgagc
     1621 cggcttgccg tcatccctga accaggagcg gatcccgttg aagtagctgc ggtgttgatg
     1681 gacggcatgg atctggtcct gctcggcctc ggcgggcgtt cggtgcctgc cacacgggcg
     1741 cgggccgtgg tggctcgcgc gcggcagaag ggttgcacgc tgttggtcac cggcggcgag
     1801 tggcagggcg cgtcggctcg gctggaggcc agggtcagcg gctacgaagt gaccggcggc
     1861 agcggcgaaa ggcccatatc gggatgcggg cggatcagca gggtgcggct ggccatgcgg
     1921 gcgagaggac gagccttcgg gtcggtgcac gcggtaggcg aatgagcaca tcgcgggtcc
     1981 tggccatctg gtgcatggat tggcctgcgg tggccgcggc ggccgcggcc gatctgcccc
     2041 cgacctctcc ggtggcggtc acgctggcca atcgagtcat cgcctgctcg gcgtcggccc
     2101 gcgcggcggg agtgcgccgc ggcctgcgcc gcagggagtc ccaggccaga tgtcctgagc
     2161 tgcacgtcgt caccgccgac ccggcgcgcg atgcccgcca cttcgagaac gtgacgacgg
     2221 cggtcgacga cctggtgcca cgcgccgagg tgctgcgacc cggcctgctg gtgctgccgg
     2281 ttcgcggcgc ggcgcgctac ttcggctccg agcagactgc tgccgagcgg ctggtcgatg
     2341 cggtggccgc tgccggaacc gaatgtcagg tcggaatcgc cgaccggctg cccactgcgg
     2401 tcttcgccgc acgcgccggg cggatcatcg agccgggcga ggacgcggtg ttcttgtccg
     2461 cgctatccat ccggcagctg gccaccgaac cgagcctggc ggcggccggg cgcgaagatc
     2521 ttgcggatct attgtggcgc atgggaatcc ggaatatcgg tcagttcgct gccttgtccc
     2581 gcgccgatgt ggcctcccgg ttcggggccg acgcgatcgc cgcacatcgc ttcgcccgtg
     2641 ccgaggccgg ccgcgggccg tcgggacgcg aaccgtcacc ggatctcgac gccgtgatgg
     2701 attgtgaccc gccggtcgac cgggtggatg ccgctgcgtt cgccggccgg tcgctggcag
     2761 gcgatctgca tcgcagcctc gaggccgcgg gcgtcggctg cacccggttg gcgatccatg
     2821 ccgtgaccgc caacggtgaa gagctgaacc gggtttggcg atgcgcggaa ccgctgaccg
     2881 aggatgccac cgccgaccgg gtgcgatggc agctggacgg ctggctgaat cgccgcaatc
     2941 cgaacgaccg gcctaccgca ccgatcaccg tgctgcggct gcaaccagtg gaagtggtct
     3001 cggcagaggc tttgcagctg ccgctgtggg gtggcattgg ggaagaggac cggctgcgcg
     3061 cccgtcgtgc gctggttcgg gttcagggtc tgctcgggcc cgaggccgtg caggtgccgg
     3121 tgctcagcgg aggccgcgga cccgccgagc gcatcacgtt gacaccgctg ggtgacgagc
     3181 cggtgccgca ggccgaccct gctcagccgt ggccgggtca actgcccgaa ccgtcaccga
     3241 ccgtgctgct cgacgatccc gtcgaagtgc ttgaccacga aggcaatccg gtgcgggtga
     3301 ccaatagggg attgttctcc gccgacccgg cgcggctggc cgccccaggc agggaaggtg
//
LOCUS       DASYLX010000066         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_552082,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000066 DASYLX010000000
VERSION     DASYLX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552082"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGP27_01695"
     CDS             complement(<1..315)
                     /locus_tag="VGP27_01695"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016890616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV7850061.1"
                     /translation="MVEIGMGRTARRTYELDDINIVPSRRTRSSQDVSTAWQLDAYRF
                     EIPVIAHPTDALVSVEFAIEMGRLGGLGVLNGEGLIGRHADVAAKIAQVVEAAEKEPE
                     PAA"
     gene            complement(334..1875)
                     /gene="guaB"
                     /locus_tag="VGP27_01700"
     CDS             complement(334..1875)
                     /gene="guaB"
                     /locus_tag="VGP27_01700"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV7850062.1"
                     /translation="MSIAESDIPIAVPVPTGGDDPTKIAMLGLTFDDVLLLPAASDVV
                     PATADTSSQLTRRVRLKVPLVSSAMDTVTESRMAIAMARAGGMGVLHRNLPVAEQAGQ
                     VETVKRSEAGMVTDPVTCSPDNTLAEVDAMCARFRISGLPVVDDTGALVGIITNRDMR
                     FEVDQHKPVSEVMTKAPLITAREGVTADAALGLLRRHKIEKLPIVDGHGRLTGLITVK
                     DFVKTEQHPFATKDRDGRLLVGAAVGIGADAWTRAMTLADAGVDVLIVDTAHAHNRMV
                     LDMVSKLKREVGHRVDVIGGNVATRAAAAALVEAGADAVKVGVGPGSICTTRVVAGVG
                     APQITAILEAVAACAPSGTPVIADGGLQYSGDIAKALAAGASTAMLGSLLAGTAEAPG
                     DLIFVNGKQFKSYRGMGSLGAMQGRGTAKSYSKDRYFQDDALSEDKLVPEGIEGRVPF
                     RGPLSTVIRQLTGGLQAAMGYTGAATIEQLQQAQFVRITAAGLKESHPHDITMTVEAP
                     NYYTR"
     gene            2023..2427
                     /locus_tag="VGP27_01705"
     CDS             2023..2427
                     /locus_tag="VGP27_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 domain-containing protein"
                     /protein_id="HEV7850063.1"
                     /translation="MRDHLPPGLPPDPFADDPCDPSGALDALEPGQPLDNQERTAVEA
                     DLADLAVYEALLAHKGIRGLVVCCDECQQDHYHDWDMLRANLLQLLVDGTVRPHEPAY
                     DPEPDAYVTWDYCRGYADASLNEATSESDGYR"
     gene            complement(2441..3685)
                     /locus_tag="VGP27_01710"
     CDS             complement(2441..3685)
                     /locus_tag="VGP27_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-D factor RsdA"
                     /protein_id="HEV7850064.1"
                     /translation="MPDFSRWGGNGGDPSLNAIARTESYLDALASEQPVYATDPADAE
                     LSNLMAAWRDEVRRPTARVAAQRDAEIVLQRALASRRRTRMSMAVTGSVAAALLCLGG
                     FGAVVYGAAPGDALYGLRVTLFGEQQVTRDDKVALAAQTEMQQVQQLIEQGNWDAAQD
                     KLSTITSTVQTVEDPQRQQELQQQLNNLTVKVESRDPEATAPQSAPLSPEAPAGPGIS
                     LPVQVPSLPNLPVPPSLPGQLPVPLPDISLPQLPIPQPPLPGPGVPPDISLPPIQLPK
                     PLPDGPKPLPDGPKPLPDGPKPLPDGPKPLPKPGSDDDSPPKPGQDQPKPPPAPDEPE
                     PAPKPVEPPKQLAPPPAPAPAPAPAPAPVEEPPPAAVIPVIPPAPKAPPALAPQQEAP
                     PQPVEPSTTLFPIPAEKPKSRN"
BASE COUNT          557 a         1260 c         1288 g          644 t
ORIGIN      
        1 cgcagcgggt tctggctcct tctccgcggc ctcgacaacc tgggcgatct tggccgcgac
       61 gtcggcgtgc cttccgatca gcccctcgcc gttgagcacg ccgagaccac cgaggcggcc
      121 catctcgatg gcgaactcca ccgacaccag cgcatcggtc ggatgggcga tgacggggat
      181 ctcgaaccgg taggcgtcga gctgccaggc ggtcgacaca tcctgcgacg accgggtccg
      241 ccgggacggg acgatgttga tgtcgtcgag ttcataagtg cgacgggcgg ttcggcccat
      301 cccgatttcg accatgtcgc gcattcgggc tcgctagcgg gtgtagtagt tgggcgcttc
      361 gacggtcatc gtgatgtcgt gcgggtggct ttccttcaac cccgccgcgg tgatccgcac
      421 gaactgcgcc tgctgaagct gctcgatggt cgccgcgccg gtgtagccca tcgcggcctg
      481 cagaccgccg gtgagttggc ggatcaccgt cgacaacggg ccacggaacg ggacccggcc
      541 ttcgatgccc tcgggcacca gcttgtcctc gctgagcgca tcgtcttgga agtagcggtc
      601 tttcgagtac gacttcgcgg tgccgcgacc ctgcatcgcg cctagcgacc ccatgccgcg
      661 gtagctcttg aactgcttgc cgttgacgaa gatgaggtca ccgggcgcct ccgcggtgcc
      721 cgccagcagt gagcccagca ttgccgtcga tgcccctgcg gccagtgcct tggcgatgtc
      781 gcccgagtac tgcaggccgc cgtcggcgat caccggagtc cccgagggag cgcatgccgc
      841 aacagcttcc agaatcgccg tgatttgtgg agcgccgacc ccggcgacca cgcgggtggt
      901 gcagatggaa ccggggccca ccccgacctt cacggcgtcg gcaccggcct cgaccagtgc
      961 ggccgccgca gcgcgggtgg cgacgttgcc accgatcaca tccactcggt ggcccacttc
     1021 gcgtttgagc ttgctgacca tgtccagcac catgcggttg tgggcgtgcg cggtgtcgac
     1081 gatcaggacg tcgacgcctg cgtcggccag cgtcatcgcg cgcgtccagg cgtcggcgcc
     1141 gatgcccacc gcggcaccga ccagcagcct gccgtcgcga tccttggtgg cgaacgggtg
     1201 ttgctcggtc ttgacaaagt ccttgacggt gatcagcccg gtcagccggc cgtgcccgtc
     1261 gacgatcgga agcttttcga tcttgtggcg ccgcaagagg ccaagggccg catccgcggt
     1321 gacgccctcg cgcgccgtga tcaacggcgc cttcgtcatc acctccgaca ccggcttgtg
     1381 ttgatcgacc tcgaagcgca tgtcgcggtt ggtgatgatt cccacgaggg cgccggtgtc
     1441 atcgacgacg ggcagcccag agatccggaa ccgcgcgcac atcgcgtcca cttcggcgag
     1501 cgtgttgtcc ggcgagcacg tgacggggtc ggtgaccatg cccgcttctg accgcttgac
     1561 cgtttccacc tggcctgcct gctcggcaac cggaaggttg cggtgcagca cgcccatgcc
     1621 gccggcgcgg gccatcgcga tcgccatccg ggactcggtg acggtgtcca tcgccgagct
     1681 gaccagcggc accttcagcc ggacccggcg ggtgagctga ctggacgtat cggccgtggc
     1741 gggcaccaca tcggaggcgg cagggagcaa cagcacgtcg tcgaaggtca acccgagcat
     1801 ggcgatcttg gtcggatcgt cgccgccggt gggcaccggt acggcgatgg ggatgtcgct
     1861 ttcagcgatc gacattggtt acagcctcca tcggcgggga gctagataac tatcttatcg
     1921 gcatcggctt cggagaaccg gccccacacg gggtcgccga gtgacccggg cccgctgggc
     1981 aaccgctagc gcgataccag taccgctgcg tagtgtggaa ccgtgcgaga tcacctcccg
     2041 cctggtttgc cgccggaccc gttcgccgat gacccgtgcg atccgtccgg agcgctcgac
     2101 gctctggaac ccggccaacc gctggacaac caggaacgca ccgcggtgga ggccgacctc
     2161 gccgaccttg cggtctatga agctctgttg gcgcacaagg gaattcgcgg tctggtggtg
     2221 tgctgtgacg agtgccagca agaccattat cacgactggg acatgctgcg ggccaatctg
     2281 ttgcagctgt tggtcgacgg caccgtgcgc ccccatgagc cggcctatga tcccgagccg
     2341 gatgcgtatg tgacctggga ttattgccgc ggatacgccg atgcttcgct caacgaggcg
     2401 acgtcggagt ccgacggcta tcgctgatag ctgaaccccg tcagttccgc gatttcggtt
     2461 tctctgcagg aatcggaaat aacgtcgtcg acggttccac cggctgaggc ggcgcctcct
     2521 gctgtggcgc tagagcgggc ggagccttgg gcgcaggcgg aatcaccgga atgaccgctg
     2581 ccggcggcgg ctcctctaca ggcgctggcg ctggcgctgg cgcaggtgcg ggcgcgggcg
     2641 gtggtgccag ttgcttcggc ggctccacgg gcttgggcgc aggctccggt tcatccggcg
     2701 ccggcggagg tttcggctga tcctgccccg gcttcggcgg cgaatcgtcg tccgagcccg
     2761 gcttcggcag cggcttcggc ccgtcgggaa gcggcttggg cccgtcggga agcggcttgg
     2821 gcccgtcggg aagcggcttg ggtccgtcgg gaagcggctt gggcagctgt atgggtggca
     2881 gcgaaatatc cggcggcaca ccgggtccgg gcagaggcgg ctgtggtatc ggcagctgtg
     2941 gcagcgagat gtccggcagc ggcacaggca actgcccggg cagcgacggc ggaacaggca
     3001 aattcggcaa cgacggcacc tgaacgggca gcgatatccc cgggccggcg ggcgcctccg
     3061 ggctcagcgg tgcggattgt ggagccgtcg cctcggggtc gcggctttcc accttgaccg
     3121 tcagattgtt cagctgctgc tgcagttcct gctgccgctg cgggtcctcg acggtctgca
     3181 cggtggaggt gatcgtcgaa agcttgtcct gagctgcgtc ccagttgccc tgctcgatga
     3241 gctgctggac ctgctgcatc tcggtctgcg cggccagcgc gaccttgtcg tcgcgggtga
     3301 cctgctgctc gccgaacagc gtgacgcgca gcccgtagag cgcatcgccg ggcgccgcgc
     3361 cgtagacgac ggccccgaac ccgccgaggc acaacaaagc agcggcgacc gagcccgtga
     3421 ccgccatgga catgcgagtc cgccgccgcg aggcgagcgc ccgctgcagc acgatctccg
     3481 catcgcgctg cgcggcgacc ctcgcagtcg gcctgcgcac ctcgtcgcgc caggccgcca
     3541 tcagattcga cagctcggca tctgccggat cggtcgcgta caccggctgt tcggaggcca
     3601 acgcgtcaag gtagctctcc gtgcgcgcga tggcgttcag cgacggatcc ccaccgttgc
     3661 cgccccagcg actgaaatca ggcatggtcg tgccccgtcg ccatgatctc cgtcttcagc
     3721 cgcgccagcg cccggtgctg tgccacgcg
//
LOCUS       DASYLX010000067         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1978596,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000067 DASYLX010000000
VERSION     DASYLX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978596"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1644)
                     /locus_tag="VGP27_01715"
     CDS             complement(238..1644)
                     /locus_tag="VGP27_01715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7850065.1"
                     /translation="MVTRLSASDASFFGLENSSTPMYVSSLSILRRPRSGLSYETLLE
                     TVEHRLPQIPRYRQKVREVRFGVARPVWVDDREFDITYHIRRSALPSPGSDAQLHELV
                     ARLGSRALDKSRPLWEMYLVEGLAGNRLAIYTKSHQALVNGMTALEIGHVIADRTQKP
                     PPFAEDIWIPAREPSDAALLMGAVGEWVARPGGQLAAVRTAVTVATNAGQLLEAARRF
                     ADVAKTFARGSAPSSPLNSKVSRNRRFTVTSGKLEDYRNLRARYDCDVNDVVLAVIAG
                     ALRNWLLSRGEPVAPTTTVRAMAPMSVYPDAEIDSSGPGQAISEVSPFLVDLPVGEGN
                     AVVRLSQIAHMTESHSTAASLVDARTIVTLSGFAPPTLHAMGIRVATTFPARQFNLLI
                     TNVPGAQKQMYIAGTKLLETYAVPPLLDSQVLAIGVTSYNGMLYFGINADRDAMSDVD
                     VMPSLLRESLDELLEAAQ"
     gene            1831..2349
                     /locus_tag="VGP27_01720"
     CDS             1831..2349
                     /locus_tag="VGP27_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HEV7850066.1"
                     /translation="MTASPIPFEPGALTAPVVDYEPPPFDIAGRATSCPAPTSAVLHR
                     SPRRASQPVERAASDVAPPPAAAAFADAALRRVLEVIDRRRPIAQLRQMLTPPLLDTV
                     FTLTRTEGRDKAAVLRRVRLRTAAVDGQDPRQPMAVEVFATYTRGERVRAIAGRVEIK
                     EGRWCLVALQIG"
     gene            complement(2384..>2819)
                     /locus_tag="VGP27_01725"
     CDS             complement(2384..>2819)
                     /locus_tag="VGP27_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV7850067.1"
                     /translation="QKDPVVEYQREGFDMFNGMLDAMKEEAVGFLFNVTVEAVPAAPA
                     AQVAPVAAPTGLAEFAAEAAAKAQQGGVATKERQPAPALRTKGIDDQAPQLTYTGPSE
                     DGSAEVQRQGANGGNGNARHAAPSRRERREAARRQAKSAKKG"
BASE COUNT          432 a          962 c          952 g          473 t
ORIGIN      
        1 gacggcgatg acggcggtga caccaaagac caccttgtcg agtccgcggt tgcgggtgtg
       61 cacctcgtcg tcgatcggca cagcgaagat cggatgcgcc gcctggccgt tcggggaggc
      121 cggcatgccg gccgccgact ctccggaagt ctcaggagat tcagagctga atgtcatggt
      181 gaattcattc gaaacctgtt gacgtcggcc gtcaagccga ccgacgcaaa caatgcgtta
      241 ctgagcggct tccagcaact cgtcgagcga ttcccgcagc aaggacggca ttacgtcgac
      301 gtcgctcatc gcgtcgcggt cggcgttgat gccgaagtag agcatcccgt tgtaggacgt
      361 gacgccgatc gccaaaacct ggctgtccag cagcggtggc accgcatagg tctcgagcaa
      421 cttggtgcct gcgatgtaca tctgcttctg ggcaccgggc acgttggtga tcagcaggtt
      481 gaactgtcgg gccgggaagg tggtcgcgac ccgaattccc atggcgtgca aggtgggtgg
      541 cgcaaagccc gacagggtga cgatggtcct ggcgtccacc aggctcgcgg ccgtcgagtg
      601 cgactccgtc atgtgcgcga tctgggacag ccgcaccacg gcgttgccct ccccgacggg
      661 caggtcgacg aggaacggcg acacctcgct gatggcctga cccggacccg aggaatcgat
      721 ctcggcgtcc ggatacaccg acatcggcgc catcgcccgc acggtggtcg tcggagccac
      781 cggctcgccg cgcgacagca gccagttgcg cagggcgccg gcgatcaccg cgagcaccac
      841 gtcgttgacg tcacagtcgt agcgggcccg caggttgcgg tagtcctcga gttttccgct
      901 ggtgacggtg aaccggcgat tgcgcgacac cttggagttc agcgggctgg acggcgcgct
      961 ccccctggca aaggtcttgg cgacatcggc gaaccggcgg gccgcctcga gcagctgccc
     1021 cgcgttggtc gccacggtga ccgcggttcg cacagcggcc agttggccgc cgggtcgagc
     1081 gacccattcg ccgacggcac ccatcagcag cgcggcgtcg ctcggctcgc gtgccggaat
     1141 ccagatgtct tcggcgaacg gcggcggctt ctgggtgcgg tcggcgatca cgtgcccgat
     1201 ctcgagcgcc gtcatcccgt tgacgagagc ctgatgagat ttggtgtaga tcgccagccg
     1261 gttcccggcc agcccctcga ccaggtacat ctcccacagg ggccgcgact tgtccagggc
     1321 acgggacccc agcctcgcca ccagctcgtg cagttgggcg tcgctgccag gagacggcag
     1381 cgcggacctg cggatgtggt aggtgatgtc gaattcgcgg tcgtcgaccc acaccggccg
     1441 ggcaacgccg aacctcacct cgcggacctt ctgccggtag cgcggaatct ggggcagccg
     1501 gtgttcgacg gtttccagca gggtttcgta gctcagcccg ctgcggggcc tgcgcaggat
     1561 cgacagcgac gagacgtaca tcggtgtgga ggagttctcc aagccgaaga aggacgcgtc
     1621 agacgccgac agcctggtca ccatgtgcgg cgccacccct cccctaggca ttgacgtcga
     1681 cgcttgtcca ccccccgaca cggtaactgt cgagtctgta tgtgcgcatc acgggtgcgt
     1741 gtccgcctcc caaaccatgg gatcatggac gtgtctgttc ctctccggca ggcgccgcac
     1801 gcaacccttg ccgaacgctg gagaggtccg ttgaccgcat cgccgattcc cttcgaaccg
     1861 ggtgccctca cagcaccggt cgtcgactac gaaccaccgc cgttcgacat tgccggccgg
     1921 gccacttctt gtcctgcccc gacgtctgcc gtgctgcacc gctcaccccg gcgcgcgtcg
     1981 cagcccgtcg agcgtgcggc ttccgacgtc gcaccgccgc cggccgcggc tgccttcgcc
     2041 gatgcggcgc tgcgccgggt actcgaggtg atcgaccggc gcaggccgat cgcgcagctg
     2101 cgacagatgc tgacgccgcc gctgctggac acggtcttca cgctgaccag aaccgagggg
     2161 cgcgacaagg cggcggtgtt gcgacgggtg cggctgcgca ccgccgccgt cgacgggcag
     2221 gatccgcggc aaccgatggc cgtcgaggtg ttcgcgacct acacccgggg agaacgcgtt
     2281 cgggccatcg ccgggcgcgt cgaaatcaag gagggtcgct ggtgcctagt ggccctgcag
     2341 atcggctgat gacggcgcat cgccgccgtg gtcgagaact agctcaaccc ttcttggccg
     2401 acttcgcctg acggcgtgcg gcctcacggc gctcgcgcct gctcggcgcg gcgtgccgcg
     2461 cgttgccgtt gccgccgtta gcgccttggc gctgcacctc agcggagccg tcttcggacg
     2521 ggccggtata ggtcagctgc ggcgcctggt cgtcgattcc cttggtgcgc aaggccggag
     2581 ccggctgacg ctccttggtc gcgacgccac cctgctgcgc cttcgccgcg gcctcggcgg
     2641 cgaattcggc cagtcccgtc ggcgcggcga ccggtgcgac ctgtgccgcg ggtgcggcgg
     2701 gcaccgcttc gacggtgacg ttgaacagga agccgacggc ctcctccttc atcgcgtcga
     2761 gcatgccgtt gaacatgtcg aagccctcgc gctggtactc gaccaccgga tccttctgc
//
LOCUS       DASYLX010000068         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2088252,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000068 DASYLX010000000
VERSION     DASYLX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088252"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1489
                     /locus_tag="VGP27_01730"
     CDS             41..1489
                     /locus_tag="VGP27_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEV7850068.1"
                     /translation="MTEQILHVIDGDEVESFSGAFFDTVDPWTREPWARVALGGAEDA
                     DRAVAAARRAFDEGPWPRMGLAERGAILHRLADLIMEHRDELALADTTDMGKPLADSR
                     DNDVPRSAQNFRFFADHARLCAGEALPMDTGHHAYTRFEPAGVVAAIAPWNFPLMLET
                     WKVAPALAWGNTVVLKPAEDTPASATILARLGLAAGLPDGVLNVVHGYGPDSVGSALT
                     GDARVDRITFTGESGTGRTIAAAASAHLTPVSLELGGKGANLVFADADLDSAVSWSIR
                     AIFSNAGQVCLAGSRLYVQRAVYDEFLARFVAAAEALTAGDPTSTGTQLGPLASEEHW
                     KKVRAYIDGIPEDGGVMRTGGLGEGWLISPTIVTDLPAGARLLRDEIFGPVVVVSPFD
                     TEADAIAAANDTPFGLNAMLFTENLSRAHRVSAALKAGTVWVNCFFIRDLRAPFGGVG
                     DSGVGREGGTFSREFFTEPKAVVMAITEGAGV"
     gene            complement(1529..>1804)
                     /locus_tag="VGP27_01735"
     CDS             complement(1529..>1804)
                     /locus_tag="VGP27_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEV7850069.1"
                     /translation="LSRAVLSQDWAGADHISEQVNWALAPQFGGDMSRFMDYSIPVGR
                     GRFRGAGLIDCGPSRPPYTDAPPDMVAGGEETGRRWAELEQKYRGTR"
BASE COUNT          296 a          607 c          604 g          297 t
ORIGIN      
        1 ccgcagggga cgtgacgatc gcggaacgtt ccaagacttc atgaccgaac agatactgca
       61 cgtcatcgac ggcgacgagg tcgaatcgtt cagcggagcc ttcttcgaca cggtcgatcc
      121 atggacccgc gagccgtggg cccgggtggc actgggcggc gccgaggacg ccgaccgtgc
      181 ggtcgcggcg gcgcgccggg cgttcgacga aggtccctgg ccgcgaatgg gtttggccga
      241 acgcggcgcg attctgcacc ggctggcgga cctcatcatg gagcaccgcg acgaactcgc
      301 gctcgccgac accaccgaca tgggcaagcc gctcgctgac agccgcgaca acgacgtgcc
      361 ccggtccgcg cagaatttcc ggttcttcgc cgaccatgcg cgactgtgcg caggcgaagc
      421 gttgccgatg gataccggcc accacgcata cacgcgtttc gaaccggcgg gtgtggtcgc
      481 cgcgattgcg ccgtggaact tcccgttgat gctcgagacc tggaaggtcg caccagcatt
      541 ggcctggggg aacactgttg tcctcaagcc tgccgaggac acccccgctt ctgcgaccat
      601 cctggctcgc ttgggtttgg cggcgggact accggatggc gtgctcaacg tcgtgcacgg
      661 atacggcccc gattcggtcg gatccgcgct taccggcgac gccagggtgg accgcatcac
      721 cttcaccggc gaatcgggca cgggcagaac cattgccgcc gcggcgtctg ctcacctcac
      781 gccggtgagc ctcgaactgg gcggcaaggg tgcaaacttg gtcttcgcgg atgccgacct
      841 cgacagcgcg gtcagctggt cgatccgggc gatcttctcc aacgccggac aggtgtgcct
      901 ggccggcagc cggttgtatg tccagcgtgc cgtgtatgac gagttcctgg cgcggttcgt
      961 cgccgcagcg gaggcgctca ccgccggcga tccgacatcg actggtacac aactcggccc
     1021 ccttgcctcc gaagagcatt ggaagaaggt ccgcgcctac atcgacggaa tccccgaaga
     1081 cggtggcgtc atgcgcaccg gcggtctggg ggagggatgg ctgatctcgc cgacgatcgt
     1141 gacggacctg ccggccgggg cgcgcctcct tcgcgacgag atcttcgggc ccgtcgtcgt
     1201 cgtctctccc ttcgacacgg aggccgacgc catagcggcc gccaacgaca cccccttcgg
     1261 cctcaacgcg atgctgttca ccgagaatct gtcgcgcgcc caccgtgttt cggccgcgct
     1321 gaaggcaggg acggtctggg tgaactgctt cttcatccga gacctacggg cgccgttcgg
     1381 aggtgtcgga gactcggggg tcggacgcga aggtgggacg ttcagccgcg agttcttcac
     1441 cgagccgaag gcggtcgtga tggccatcac cgaaggtgct ggcgtctgaa tctgcgcgga
     1501 cgtcccgcac agaaaaggcg ctgaactctc agcgggtccc gcggtacttc tgctccaatt
     1561 cggcccaccg ccggccggtc tcctcgcccc ctgcgaccat gtcgggtgga gcatcggtgt
     1621 acgggggccg gctgggtccg cagtcgatga gacccgcccc gcggaaccgg ccgcgaccga
     1681 ccgggatgct gtagtccatg aaacgcgaca tgtcgccgcc gaactgaggt gccaacgccc
     1741 aattcacctg ttcggaaatg tgatccgcgc cggcccagtc ctgggacagc accgcgcggg
     1801 acag
//
LOCUS       DASYLX010000069         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1649491,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000069 DASYLX010000000
VERSION     DASYLX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649491"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VGP27_01740"
     CDS             <1..696
                     /locus_tag="VGP27_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV7850070.1"
                     /translation="TVVQPLVGHLFGGRLIHEQPAKLVAIELDPTTEQPAPLRIGGLL
                     IDGDVRWALPIPRLGSLLTQDSWDAPVPGIDTVPPGDRPPINITHLAFQTMVGVALLL
                     LAAVIVYWVARRRGRDLLANRWFLRFMVIAGPLAVLALECGWTTTEVGRQPWTVWRVL
                     RTTDAVTMNPGLWWTYGGVLVIYTGMTVGAYIVLTSMARRWRAGAPVSSPYGPALSQG
                     ERVATEGGARSDS"
     gene            693..1721
                     /locus_tag="VGP27_01745"
     CDS             693..1721
                     /locus_tag="VGP27_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV7850071.1"
                     /translation="MILSAAVAAALFVGVIPYALMGGADFGSGFYDLTAGGGVGGAQL
                     RALIEESMGPVWEANHVWLIYIIVMWWTGFPAAFAAATSTLFIPLAAALAGIVLRGAS
                     FAFRNFAASSGQARLFSAVFAAASLITPFFLGTVAGALASGRVPVHGYGPLVGSWANP
                     TSLTGGALAVAAGVFLAGVYLTDDASRAGLTHLADRLRRRTLIIGILVGLIVLGGVYP
                     IFHDAPTLSHGLLGRALPLVIIAALAGVLTLALLRRRASRPARFSAAVAVGAAVAGWG
                     VAQYPWMFADQLTIQEAAGASTTLQALIVVVAFAAVIVLPALAYLLWFTQKSKSTLVD
                     GHSAASST"
     gene            1943..2851
                     /locus_tag="VGP27_01750"
     CDS             1943..2851
                     /locus_tag="VGP27_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV7850072.1"
                     /translation="MITLYALAAAFGIGVLWLGTNIKVVKQFERGVVFRFGRVQSGLR
                     GPGLTMLIPIADHLEKVNMQIITMPVPAQDGITRDNVTVRVDAVIYFKVSDPVRAAVD
                     VQDYPSAIGQVAQTSLRSIIGKSNLDDLLSNREHLNQGLELMIDSPALGWGVQIDRVE
                     IKDVVLPDSMKRSIARQAEAERERRARVITAEGELQASQKLAQAAEVMAEHPAALQLR
                     LLETVVEVAAEKNSTLVLPFPVELLRFLERATPPAPAGQNKATDPDADAASNRETMTL
                     EPGRIPDDARGLELHTRIAETFQRDQ"
     gene            complement(2897..3478)
                     /locus_tag="VGP27_01755"
     CDS             complement(2897..3478)
                     /locus_tag="VGP27_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850073.1"
                     /translation="MLGYHEVAEAMRDPQIPVLLERFLDKVVVPLLEAPRDVDLVDYK
                     DTVLDRFSNEAMHDQLTRIASDSASKVPVFLTTTIQQVLALGADHRIPAFILAAWSRV
                     LQGEDDDGKGFDVTEPRLSEYARRSMIVGDPREALSIEPLLASGAAEHADFVASFEHY
                     RRTLAESGAEAVLKAVLDATAEDQAATAVPGSR"
BASE COUNT          578 a         1095 c         1124 g          698 t
ORIGIN      
        1 accgtggtgc agccgttagt cggacacttg ttcggcggcc ggctgatcca cgaacaaccg
       61 gccaaactgg tggcgatcga actcgacccg accaccgagc agccggcgcc gctgcgaatc
      121 ggcgggctgc tgatcgacgg ggatgtccga tgggccctgc ccatcccgag gttggggtca
      181 ttgctcacgc aggattcgtg ggacgccccg gtccccggga ttgacaccgt gccgcccggc
      241 gatcgtccgc cgatcaatat cacgcacttg gcttttcaga cgatggtggg cgttgcactg
      301 ttgcttctgg cggcggtgat cgtgtactgg gttgcccgac ggcgcggccg cgacttgctt
      361 gctaaccgat ggtttctccg cttcatggtc atcgccggtc cgctggccgt actcgccctt
      421 gagtgcggat ggaccaccac ggaggtcggg cgtcaaccgt ggacagtgtg gcgtgtgctg
      481 cggacgacag acgccgtcac catgaaccct gggttgtggt ggacctatgg tggtgttctc
      541 gtgatctaca cgggcatgac tgtcggcgcc tacatcgtgc ttacctcgat ggcgcgtcgc
      601 tggcgcgccg gggcgccagt gtccagcccc tacgggccgg ccctctcgca gggcgagcgc
      661 gtcgccaccg agggcggggc aaggtccgat tcatgattct gtctgcagcg gttgccgcgg
      721 ccctcttcgt cggcgtcatc ccttatgcct taatgggagg cgctgacttc ggctctggct
      781 tttacgatct gacggccggg ggtggtgttg gtggggcgca gcttcgtgca ctgatcgagg
      841 agagcatggg gccggtgtgg gaagccaacc acgtgtggtt gatctacatc atcgtcatgt
      901 ggtggaccgg tttcccggct gcgttcgcgg ccgccaccag tacgctgttc attccgctgg
      961 cagccgcatt ggccggcatc gtgctgcgtg gcgcaagttt tgccttccgc aacttcgcgg
     1021 cctcctctgg tcaagctcga ctattcagcg cggtgttcgc cgcagcatcg ctgatcaccc
     1081 cgttctttct cggaacggtg gcgggggcac ttgcttctgg gcgggttccc gtgcacggct
     1141 acggtccgtt ggtcggatca tgggcaaatc ccacatcgct gacgggtggg gcgctggcgg
     1201 tggcggcggg cgtttttctc gccggggtct atctgaccga cgatgcgtca cgcgcggggc
     1261 tgacccacct tgccgaccgt ttgcgacgcc gtacgttgat catcgggatc ctggtggggc
     1321 tcatcgtgct cggcggcgtg tatccaatct ttcacgacgc tcccacgctc agccacggct
     1381 tgctcggccg cgcgttgccg ttggtgatca tcgccgcgct ggccggtgtt ctcacgttgg
     1441 cactgctgcg tcggcgcgcc tctcggccgg cgcggttttc ggccgcagtg gccgtcggcg
     1501 cggcagtcgc cggatgggga gtcgcacagt atccctggat gtttgccgac caactgacca
     1561 tccaggaggc ggcaggtgcg agcaccactc tgcaagccct aatcgttgtc gtcgcattcg
     1621 ccgcagtcat cgtgctgccg gcgctggctt acctgctctg gttcactcag aagtcgaaat
     1681 caactcttgt cgatggacat agcgccgcgt cgtcgacgtg acggactgtc atctcagacc
     1741 aaggtcgcac ccaatcaaga attctgcgtg aggtcgccat ggctgtggga gtcctccgct
     1801 gcaaggagca cacgtgtttg ctggggcgga gagccgagca gggtcactcg cattgtcgga
     1861 aggagaggcg tttcatcggt agtagcagcc cgagctcgct gacgagcacc ggcagccgac
     1921 gccgggcaga ttggagctgg aaatgatcac cctctacgca ctggccgcgg ccttcggcat
     1981 cggtgtgctc tggctcggaa caaatatcaa ggttgtcaag cagttcgaac gcggtgtcgt
     2041 cttccggttc ggccgtgtgc aatccggcct tcgcggacca ggactcacaa tgttgattcc
     2101 gatcgccgat cacctcgaaa aagtgaacat gcagatcatc acgatgcctg tgcccgccca
     2161 agatggcatc acccgcgaca atgtgaccgt ccgtgtggat gctgtcattt atttcaaggt
     2221 ctcggatccg gtgcgtgcgg ctgtcgatgt gcaggactac ccgtcggcta tcggccaggt
     2281 tgcacagaca tcgctgcgtt cgatcatcgg caagagcaac ctggatgacc tgctgtccaa
     2341 ccgcgagcat ctcaatcagg gcctggagtt gatgatcgac agccccgcac tggggtgggg
     2401 tgttcagatc gaccgcgtcg agatcaaaga tgtagtgctg cccgattcga tgaagcggtc
     2461 gatcgcgcgc caagccgagg cggagcgtga gcgacgggct cgggtgatca ccgccgaagg
     2521 cgaacttcag gcgtcccaaa aactcgccca agcagccgag gtcatggccg agcacccagc
     2581 cgcgctgcaa ctgcgactcc tggaaaccgt cgtcgaggtc gccgcagaga agaattccac
     2641 cctggtactt cccttcccgg tcgaactgct ccgatttctg gaacgcgcca caccgccggc
     2701 acccgcgggc cagaacaagg ccacagatcc tgacgcggac gccgcgtcca accgcgaaac
     2761 aatgacgctg gaaccaggac ggattcccga cgacgcccgc ggcttggaac tccacacccg
     2821 catcgcggag acattccagc gggatcagtg acgtttgatt tgtcgcagtg cttccacatg
     2881 cttgcggtga ttcgtcttaa cggctgcccg gaaccgctgt cgccgcttgg tcttctgccg
     2941 tcgcgtccaa gacagccttc agcaccgcct cggcgcccga ctccgccagc gttcgtcggt
     3001 agtgctcgaa cgacgcgacg aaatcggcgt gctcggccgc cccgctcgcc agcaggggct
     3061 cgatactcag cgcttcgcgc ggatcaccga ctatcattga gcgacgggca tattcagaga
     3121 ggcgcggctc ggtcacatcg aagcccttgc cgtcgtcatc ctcgccctgc agcacgcgac
     3181 tccaggcggc gaggatgaac gccggaatgc ggtgatctgc accgagtgca agtacctgct
     3241 ggatggtggt cgtcaggaac accggcacct tcgatgcact gtcggaggcg atacgggtga
     3301 gctgatcgtg catggcttcg ttggagaagc ggtccagcac cgtgtccttg tagtccacta
     3361 ggtctacatc gcgcggcgcc tcgagcagcg gcacaacgac cttgtccaga aagcgttcca
     3421 gcagcacggg tatctgcggg tcccgcatgg cctcggcgac ctcgtggtag ccgagcaaca
     3481 gggcgggata ggtca
//
LOCUS       DASYLX010000070         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3075264,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000070 DASYLX010000000
VERSION     DASYLX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075264"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VGP27_01760"
     CDS             complement(<1..131)
                     /locus_tag="VGP27_01760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV7850074.1"
                     /translation="MIRIGLTGGIGAGKSTVSAAFSECGGIIVDGDVIAREVVEPGT"
     gene            complement(147..1589)
                     /gene="rpsA"
                     /locus_tag="VGP27_01765"
     CDS             complement(147..1589)
                     /gene="rpsA"
                     /locus_tag="VGP27_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV7850075.1"
                     /translation="MPSPSVTSPQVAVNDIGSAEDFLAAIDKTIKYFNDGDIVEGTIV
                     KVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPAEVVSVGDEVEALVLTKEDKEGRLIL
                     SKKRAQYERAWGTIEELKEKDEAVKGTVIEVVKGGLILDIGLRGFLPASLVEMRRVRD
                     LQPYIGKEIEAKIIELDKNRNNVVLSRRAWLEQTQSEVRSEFLNQLQKGAVRKGVVSS
                     IVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVQVGDEVTVEVLDVDMDRERVSLSL
                     KATQEDPWRHFARTHAIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELAERHVEVP
                     DQVVAVGDDAMVKVIDIDLERRRISLSLKQANEDYTEEFDPSKYGMADSYDEQGNYIF
                     PEGFDAETNEWVEGFEKQRDEWEARYAEAERRHKMHTAQMEKFAAAEAAEAEKPSANG
                     APRSEEQSAGGSLASDAQLAALREKLAGSA"
     gene            1835..2968
                     /locus_tag="VGP27_01770"
     CDS             1835..2968
                     /locus_tag="VGP27_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017206230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV7850076.1"
                     /translation="MPPYPPIQKKVRKVGAPLAVLILLGTIAGLIVIALTAFNPVGTA
                     IGFVLSSLAMTVVVLAYLWLDRWEPEPPRLLVLAFLWGASVAVVIASILQLVAEAAIN
                     PGASESVSPVTIVLGAPLTEEAAKGLFLLLMMTGRRRNELNSLTDCLVYAGLVGAGFA
                     WLEDILYIGGGETVGDSLLTAAMRLIMAPFAHSLFTTFFGIGVWFALQQRNALAKFGY
                     ILLGYLGAVIMHGLWNGSSLLGVGAYFGVYLLWMVPIFGLVIWLGVASRRKEQRIVAE
                     KLPGMVAAGLLTPNEATWLGSIRDRKAAIGQASRTGGKPAAKSVKTFAAQVVELAFVR
                     DRIDRGFGDQRVNALLTEETYAVYGARVAAPVLQALAGYRAGR"
     gene            complement(2965..>4081)
                     /locus_tag="VGP27_01775"
     CDS             complement(2965..>4081)
                     /locus_tag="VGP27_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEV7850077.1"
                     /translation="FADQIRDAAEAAYGVIGKQINLGSPKQLQVVLFDELEMPKTKRT
                     KTGYTTDADALQSLFDKTGHPFLQHLLAHRDVTRLKVTVDGLLNSIASDGRIHTTFNQ
                     TIAATGRLSSTDPNLQNIPIRTDAGRRIRDAFVVGAGFEELMTADYSQIEMRIMAHLS
                     HDEGLIEAFRTGEDLHSFVASRAFSVPIDEVTAELRRRVKAMSYGLAYGLSAYGLAAQ
                     LKISTEEAKVQMEQYFDRFGGVRDYLRDVVDQARKDGYTSTVFGRRRYLPELDSSNRN
                     VREAAERAALNAPIQGSAADIIKVAMIEVDRALKDAGLKSRMLLQVHDELLIEVAQGE
                     RDDVEKLVRDKMGSAYPLDVPLEVSVGYGRSWDAAAH"
BASE COUNT          620 a         1327 c         1331 g          803 t
ORIGIN      
        1 tcggtgccgg gctcgacgac ttccctggcg atcacgtcgc cgtcgacgat gatgccgccg
       61 cactcgctga atgcggcgga caccgtcgat ttaccggcgc cgattccgcc ggtcagaccg
      121 atacgaatca tccggtcaaa cgccgattag gcgctgccgg ccagcttctc ccggagtgcg
      181 gccagctgcg cgtcgctggc cagcgacccg cctgcggact gctcctcgga ccgcggcgca
      241 ccattggccg acggcttctc ggcctctgct gcttcagcgg ccgcgaactt ctccatctgc
      301 gcggtgtgca tcttgtgcct gcgctcggcc tcggcgtagc gggcctccca ctcgtcgcgc
      361 tgcttctcga agccctcgac ccattcgttg gtctcggcgt cgaagccctc ggggaagatg
      421 tagttgccct gctcgtcgta gctgtcggcc atgccgtact tgctggggtc gaactcctcc
      481 gtgtagtcct cgttggcctg cttgagtgac agtgagatgc ggcgacgctc gaggtcgatg
      541 tcgatcacct tgaccatcgc gtcgtcgccg acggcaacca cctggtcggg tacctccacg
      601 tggcgctcgg ccaactcgga gatgtgcacc agcccctcga tgccctcctc gacgcggacg
      661 aacgcgccga acggcaccag cttggtgacc ttgcccggca cgatctggcc gatcgcgtgg
      721 gtacgggcga agtgccgcca cggatcttcc tgggtcgcct tgagcgacaa cgacactcgc
      781 tcgcggtcca tgtcgacgtc gagcacctcg acagtgacct cgtcgccaac ctgaacgacc
      841 tcggacggat ggtcgatgtg cttccaggac agctcggaga cgtgcaccag gccgtcgaca
      901 ccgccgaggt cgacgaatgc gccgaagttg acgatggagc tgacgacacc cttgcggacg
      961 gcgcccttct gcagttggtt gaggaactcg ctgcgcacct cggactgcgt ctgctccagc
     1021 caggcacggc gggacagcac cacgttgttg cggttcttgt cgagctcgat gatcttggcc
     1081 tcgatctcct taccgatgta cggctgcaga tcgcggacac ggcgcatctc gaccagcgac
     1141 gcaggcagga agccgcgcag gccgatgtcg aggatcaggc cgcccttgac gacctcgatg
     1201 acggtgccct tgacggcctc gtccttttcc ttgagctctt cgatggtgcc ccaggcgcgc
     1261 tcgtactgtg cgcgcttctt ggacaggatc agacggcctt ccttgtcctc cttggtgagg
     1321 acaagggctt cgacctcgtc gccgacggac acaacctcag cggggtcgac gtcgtgcttg
     1381 atggaaagct cgcgggaagg gataacacct tcggtcttgt agccgatgtc gagcaagacc
     1441 tcgtcgcggt ccaccttgac gatggtccct tcgacgatgt cgccatcgtt gaagtatttg
     1501 atggtcttgt cgatggcggc gaggaagtcc tcggcagagc cgatgtcgtt gacggctact
     1561 tgcggcgagg tgacggaggg gcttggcatg tggtgggttg ctccggacag gttgggtcgt
     1621 agggacttaa gggacttttg ttattgacgt gacccgcagc agagatcgca aaaccacaca
     1681 cgggtacccg tcgaggttac tcgactgtgt acatgctgga caaacccggt ctcctcagct
     1741 ggcgaatcgc gatcaacgac cgcagccggg cgccgctcac cgataccctg ctctggtgtc
     1801 gtatgccggt ccgccgcagc agccacgtca attcattccg ccgtatccgc cgatccagaa
     1861 gaaggtgcgc aaggtcggcg cgccgctggc tgtgctgatc ctcctgggca cgattgcagg
     1921 tctcatcgtg atcgcgctga ccgcgttcaa tccggtcggg acggcgatcg gattcgtcct
     1981 gtccagcctc gcgatgacag tcgtcgtgct ggcctacctc tggttggacc ggtgggagcc
     2041 ggaaccaccg cgactgcttg tgctggcctt cctgtggggc gcctcggtcg ccgtcgtcat
     2101 cgcctcgata ctgcagctgg tcgccgaggc cgcgatcaac cccggtgcgt cggaatcggt
     2161 cagcccggtg accatcgtgc tcggcgcgcc actcacggag gaggcggcca aaggcctgtt
     2221 cctgctgctg atgatgaccg gacggcgccg caacgagctc aactcgctga ccgactgcct
     2281 ggtgtacgcc ggcctggtgg gcgccggatt cgcatggctg gaggacatcc tctacatcgg
     2341 tggcggagag acggtcggcg actcgctgct gaccgcggcg atgcggctca tcatggcgcc
     2401 gttcgcccac tcgctgttca ccacattctt cgggatcggc gtgtggtttg cgctgcaaca
     2461 acgaaacgcc ctcgccaagt tcggctatat cctgctcggc tacctcggcg cggtgatcat
     2521 gcacggtctg tggaacggct cgtccctgct cggtgtcggg gcgtacttcg gggtgtacct
     2581 gctgtggatg gtgccgatct tcgggctggt gatctggctg ggtgtggcca gcaggcgcaa
     2641 ggagcagcgg atcgtcgccg agaaactgcc cggcatggtg gcggcgggcc tgctgactcc
     2701 caacgaggcc acctggttgg gctccatccg cgaccgcaag gcggccatcg gccaagcgag
     2761 ccggaccggc ggcaagccgg ctgccaaatc ggtcaagacg ttcgccgccc aggtggtcga
     2821 gctggcgttc gtccgcgacc gcatcgaccg tggcttcggg gaccagcggg tgaatgcgtt
     2881 gctgaccgag gagacctacg cggtctatgg cgcccgggtg gctgcgccgg tgctacaggc
     2941 ccttgcgggg tatcgcgctg gtcgttagtg cgcggcggcg tcccagctcc ggccgtagcc
     3001 gaccgatacc tccagcggca cgtcgagcgg gtatgcgctt cccatcttgt cgcggaccag
     3061 tttctcgacg tcgtcgcgtt ccccttgcgc gacctcaatg agcaattcgt cgtgtacctg
     3121 cagcagcatg cgtgacttca gcccggcgtc cttgagcgcc ctgtcgacct cgatcatcgc
     3181 caccttgatg atgtcggcgg cgctgccctg gatcggggcg ttcagcgcgg cccgctcggc
     3241 cgcctcgcgg acattgcggt tgctgctgtc caactccggc aggtaccgcc tcctgccgaa
     3301 caccgtcgag gtgtagccat ccttccgggc ctgatcgacg acgtcccgga ggtagtcgcg
     3361 caccccgccg aaccggtcga agtactgctc catctgcacc ttggcctctt cggtggagat
     3421 cttcagttgc gcggcgaggc cgtaggcgct caatccgtac gccagcccgt aggacatcgc
     3481 cttgacccgg cgcctcagtt cggcggtgac ctcgtcgatc ggaacggaaa acgcccgcga
     3541 tgcgacgaac gagtgcaggt cctcaccggt gcggaacgct tcgatgaggc cctcgtcatg
     3601 cgacagatgc gccatgatcc gcatctcgat ctggctgtag tcggccgtca tcagctcttc
     3661 gaagcctgct cccacgacga acgcgtcgcg gatccggcgg ccggcatcgg tgcggatcgg
     3721 gatgttctgc aggttcggat cggtcgacga cagccggccc gtcgcagcga tggtctggtt
     3781 gaacgtggtg tggatccgac cgtccgaggc gatcgagttg agcagcccgt caacggtgac
     3841 cttcagccgg gtgacgtcgc ggtgcgcgag caggtgctgc aggaacgggt ggcccgtctt
     3901 gtcgaacagc gactgcagcg cgtcggcgtc ggtggtgtaa ccggtcttgg tgcgcttggt
     3961 cttcggcatc tcgagctcgt cgaacaacac cacctgcagc tgtttgggcg agccgaggtt
     4021 gatctgcttg ccgatgactc cgtaagccgc ttcggcggcg tcccggatct ggtcggcgaa
     4081 c
//
LOCUS       DASYLX010000071         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3514357,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000071 DASYLX010000000
VERSION     DASYLX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514357"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGP27_01780"
     CDS             complement(<1..338)
                     /locus_tag="VGP27_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV7850078.1"
                     /translation="MTTRVDEFEDLRSHLLAVAYRLTGTFADAEDAVQDAWLRWNALG
                     QKRDEIADLRAWLTTVVSRLSVDRLRSATHRRETYVGEWLPEPVVTGLEGNDPLAAVV
                     AGEDARFAAMV"
     gene            379..922
                     /locus_tag="VGP27_01785"
                     /pseudo
     CDS             379..922
                     /locus_tag="VGP27_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472616.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
     gene            1207..2553
                     /locus_tag="VGP27_01790"
     CDS             1207..2553
                     /locus_tag="VGP27_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7850079.1"
                     /translation="MSAMYAVTDPSTGEVVKEYPTATGEQIEAAVAAASKAHRDWSRG
                     STVADRAALIRRVAELHNERKEELAKIINREMGKPIDQSEGEVEFSAAIYEYYADNAE
                     KFLADEPIDLLDGEGSALIRRSSVGVLLGIMPWNYPYYQVARFAGPNLTLGNTIVLKH
                     APQCPQSAAAIEQIFTDAGYPEGAYVNIYATNDQVADIIADPRVQGVSLTGSERAGAA
                     VAEVAGRNLKKVVLELGGSDPFIVLSSDDLDSTVEAAIEGRFENTGQACNAAKRIIVS
                     DTIYDDFLDKFTKKVLEKADGLAPLSSVAAAERLDDQVKRAVDDGATLVSEGERKGAH
                     FPPGVLTNVSPDSPSYKEELFGPVATVYKVGSEDEAVQIANDTPFGLGSYVFTTDDDQ
                     AKRVADRIEAGMVFVNAVGAEGVELPFGGVKRSGFGRELGRYGIDEFVNKKLIRIT"
     gene            complement(2546..>2972)
                     /locus_tag="VGP27_01795"
     CDS             complement(2546..>2972)
                     /locus_tag="VGP27_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HEV7850080.1"
                     /translation="LTPRGVPAYAILFSSIVGFLCVIAAAFFEKSIFAFLLNSSGAII
                     LFVYILIAISQIVLRYRNGDGELRLKMWLFPVLSILTALAMLAILVQMFLDDELRWQL
                     VLSLLSWAVVLGLFFLNKRFNTSHHIDADDAEAKARALR"
BASE COUNT          527 a          992 c          929 g          524 t
ORIGIN      
        1 accatcgccg cgaacctcgc gtcctcgccg gccaccaccg ccgcgagcgg atcgttgcct
       61 tcgagccccg tcaccaccgg ctcgggcagc cattcgccga cgtaggtttc gcgccggtgc
      121 gtcgccgagc gcaatcggtc gacgctcagc ctgctaacca ccgtcgtcag ccacgcacgc
      181 agatcggcga tctcatctcg cttctgcccc aacgcattcc accgcagcca ggcgtcttga
      241 acggcgtctt ccgcgtcggc gaaggtgccc gtgaggcgat aggccaccgc cagcagatgt
      301 gagcgcagat cttcgaactc gtcgacgcgg gtggtcaccc cgctgagctt aaacccgcgc
      361 ttctgacagc cggttggcag cgttctgccg accgcgcgcg gaaccctttc gatggcggtg
      421 tacgaggcaa tcccgaagcg attcgcgcgg gttcaccctc gcttcatgac gcccgccttc
      481 ggcaccatcg tgatgggact ctccgcactc ttcttctacc tgctgctgac gttcctgtcg
      541 gagaacgcgc tcgccgactc cgtcgcctcg ctgggtcttg cggtggcgtt ctactacggc
      601 atcaccgcgt tcgcgtgcgt gtggtacttc cgcaagtcgc tgttctcgtc ggcgcgaaac
      661 atgttcttcc gcggcctttt cccgttgctc ggcggcatcg cgatggcgct ggccttcggc
      721 cagagcgcct acgacatgat ccagccggac tacggctaca ccgcattcgg tccgatcggc
      781 ggagtctccg tgctgggcgt cggcatgctc gtgctcggca tcccgttgat gctcgcctgc
      841 ttcgcgttcg gcatcaagcg gttcttccgt ggcgagacgc tgaaccccca acaccgaagt
      901 caaggtcccg gacctctact aaagtgctgg acaccgacgt cctgatgggc gggctcgcgc
      961 cggttcgccg cgcctcggcc gatatttctc ggctcgggcc ggagcccgca tcctggcggg
     1021 caccgatgcg ccggtgatcg cggtgccggg tggcgagggc taggcggcct cggtcaatct
     1081 gctgactgga attcgtggcg ttcgttgcac tcagcgactg atttcgtgca cgattgcgct
     1141 gtggagctgg gaatgcgttg attcgcttcc cgaccgacct gcctgtcttc taaggagcac
     1201 acgatcatga gcgcgatgta tgccgtaacc gacccgtcga cgggcgaggt ggtcaaggag
     1261 tacccgaccg cgacaggcga gcagatcgaa gccgccgtcg cggccgccag caaggcgcac
     1321 cgcgactggt cgcggggttc gacggtggcc gaccgcgccg ctctcatccg tagggtcgcc
     1381 gaactccaca acgagcgcaa ggaagaactc gcgaagatca tcaaccgcga aatgggcaag
     1441 ccgatcgacc agtccgaggg cgaggtcgaa ttcagcgcgg cgatctacga gtactacgcc
     1501 gacaacgccg agaagtttct cgccgacgaa cccatcgacc ttctcgacgg cgaaggctcg
     1561 gcactcatcc ggcgcagttc ggtcggtgtg ctgctgggca tcatgccgtg gaactacccc
     1621 tactaccagg tcgcccgctt cgcggggccc aacctgacgc tgggcaacac catcgtgctc
     1681 aagcacgcgc cgcagtgccc ccagtcggcg gcggccattg agcagatttt caccgacgcc
     1741 ggctatcccg aaggcgccta cgtcaacatc tacgcgacca atgaccaggt cgccgacatc
     1801 atcgccgacc cacgcgtaca aggggtttcg ctgaccggct ctgagcgtgc cggtgccgcc
     1861 gtcgcggagg tcgccggccg caacctcaag aaggtcgtcc tcgaactggg tggctcagac
     1921 ccgttcatcg tgttgagctc cgacgacctc gattccacgg tggaggccgc catcgagggc
     1981 cgcttcgaga acactgggca ggcctgcaac gccgcaaagc ggatcatcgt ctccgacacc
     2041 atttacgacg acttcctgga caagttcacc aagaaggtgc tcgagaaggc cgacggcttg
     2101 gctccgttgt cgtccgtcgc ggcggccgaa cggctggacg atcaggtgaa gcgagccgtc
     2161 gacgacggtg cgaccctggt ctccgaggga gagcgcaagg gtgcccactt ccctccgggt
     2221 gtgctcacca acgtctcgcc ggactcaccg tcgtacaagg aagagctgtt cggaccggtc
     2281 gcgacggtgt acaaggtcgg ctccgaggac gaggccgtgc agattgccaa tgacacccca
     2341 ttcgggctgg gctcctatgt cttcaccacc gacgatgatc aggccaagcg ggtcgccgac
     2401 cggatcgagg cgggcatggt gttcgtcaac gccgtcggcg cagaaggtgt cgagctgccc
     2461 ttcggcggcg tgaagcgctc aggtttcggc cgcgagctgg gccgctacgg catcgacgag
     2521 ttcgtcaaca agaagctgat ccgtattacc tgagcgcgcg cgccttcgcc tccgcgtcgt
     2581 ccgcgtcgat gtgatggctc gtgttgaacc gcttgttgag gaagaacagt cccaacacca
     2641 ccgcccagga caagaggctc agcaccagct gccaccgcag ttcgtcgtcg aggaacatct
     2701 gcacgagtat cgccagcatg gcgagcgcgg tgaggatcga caacaccggg aacagccaca
     2761 tcttcagtcg cagttcgcca tccccgttgc ggtagcgcag cacgatctgc gatatcgcga
     2821 tcaggatgta gacgaacagg atgatcgcgc cgctcgagtt cagcaggaac gcgaagatgc
     2881 tcttctcgaa gaaggcggcg gcgatcacgc acaggaagcc gacgatcgac gagaacagga
     2941 tcgcgtacgc gggcacgccg cgaggggtga gc
//
LOCUS       DASYLX010000072         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_662063,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000072 DASYLX010000000
VERSION     DASYLX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662063"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VGP27_01800"
     CDS             <1..912
                     /locus_tag="VGP27_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7850081.1"
                     /translation="PSTGIDFSVFSFSGGGVTPSAVLAGVVAAFLSWAGFEACASMGE
                     ETDNPGRNIPRALAGTLLLTGVLFVVVMFAQVNGFGTDQAGLDAFQSSGNTLGDLGTT
                     YVGQWFGLVVIFTAIVSAFGCHLATSATSGRMLYAFARDGFGPKALAHIHAATGGPRR
                     ATWLVVIVALVVNLICAAVGWPDMGTGNDAIDTYFLFAVAGSVCLMVCYLLVEIAAAW
                     FVGAPKFLDVHGGKGKVPGLVLPLLGALVIVVVLWFNVKDAETWSAAPLLGLYWCLVG
                     LIIALAASGIAKRVGESLSAELDLKSP"
     gene            909..>1784
                     /locus_tag="VGP27_01805"
     CDS             909..>1784
                     /locus_tag="VGP27_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7850082.1"
                     /translation="MSTLYERDEASIAGIEKLRFFPLEVVSGHGSTLVTPDGRELLDL
                     SATWTASGLGHGHPAVAEAVSRAVRTAPGAGGLSAVHPDSVGLAEDLLALVPGSGERR
                     VYLGHAGSDACDVALRACRHATGKRTVVAFEHSYHGGVGVAMGVSGVHVDAGATPDAD
                     VVFLPYPNPFRPTSGGIDADVAACLDLAEKHLATGAVACLIVEPILSDGGLVVPPDGF
                     LAQLHERCRAHGVPMICDEVKMGLGRPGTMHAFEHDGVVPEIVTFGKVLGGGLPLSAA
                     VGPAEILDEPPAAALL"
BASE COUNT          234 a          605 c          614 g          331 t
ORIGIN      
        1 ccgtcgacgg gtatcgactt ctcggtgttc tccttctccg ggggcggcgt cacaccttcg
       61 gcggtgctcg caggcgtcgt cgcggcgttc ctgtcctggg cgggtttcga ggcgtgcgca
      121 tcgatgggtg aggagaccga caaccccggt cgcaacatcc cgcgggcgct ggcaggcact
      181 ctgctgctga ccggcgtgct gttcgtcgtc gtgatgttcg cacaggtcaa cggtttcggc
      241 accgaccagg ccggcctcga tgccttccag agttcgggaa acacgttggg cgacttgggc
      301 accacctacg tcggtcagtg gttcggcctt gtcgtcatct tcaccgcgat cgtgtcggca
      361 ttcggctgcc acctggcgac gtcggcgacc tcgggccgaa tgctctacgc gttcgcccgc
      421 gacggcttcg gcccgaaggc gctggcgcac atccacgccg ccaccggggg cccacgccgc
      481 gccacctggc tggtcgtcat cgtcgcgttg gtggtgaacc tcatctgcgc cgccgtcggc
      541 tggccggaca tgggcaccgg caacgacgcg atcgacacct acttcctgtt cgccgtcgcc
      601 ggttcggtct gcctgatggt gtgctacctg ctcgtcgaga tagccgcggc ttggttcgtc
      661 ggcgcaccga agttcctcga tgtacacggt ggcaagggca aagttccggg cctggtgctt
      721 ccgctgctcg gcgcgctggt gatcgtcgtg gtgctgtggt tcaacgtcaa ggacgccgaa
      781 acatggtcgg cggcaccgtt gctagggctc tactggtgcc tcgtcgggtt gatcatcgcg
      841 ctcgccgcct cgggcatcgc caagcgcgtc ggcgagtcgc tgtccgccga actcgatctg
      901 aagtcgccgt gagcaccctc tacgagcggg acgaagcctc gatcgcgggt atcgagaagc
      961 tgcgcttctt cccgctcgag gtggtgtccg gacacggctc gacgcttgtc actcccgacg
     1021 gccgcgaact gctcgacctt tcggccacct ggacggccag cgggctcggg cacggccacc
     1081 ccgcggtcgc cgaagcggtc agcagggcag tccgcaccgc gcctggcgca ggcgggcttt
     1141 cggcggtgca tcccgattcc gtcggccttg ccgaggatct gctggcgctg gtgccgggaa
     1201 gcggtgaacg gcgagtctat ctgggacacg cgggatctga cgcgtgcgat gtcgcgctgc
     1261 gggcctgcag acacgcgacg ggcaagcgca cggtggtcgc gttcgagcac agttatcacg
     1321 gcggtgtcgg cgtcgcgatg ggagtctcgg gcgtgcatgt cgacgccggg gcgacaccgg
     1381 atgccgacgt tgtcttcctc ccctacccca acccttttcg gcccaccagc ggcggcatcg
     1441 atgcagacgt cgccgcgtgc ctcgacctcg ctgagaagca cctcgcgacc ggggccgtcg
     1501 cctgcctgat cgtcgagccg atcctttccg acggcgggct ggtggtgccg ccggacgggt
     1561 tcctggcgca actgcacgaa cgctgccgtg cccacggcgt gccgatgatc tgcgacgagg
     1621 tcaagatggg cctcggacgt cctggcacca tgcacgcctt cgagcacgac ggcgtggtac
     1681 ccgagatcgt gacgttcggc aaggtgctcg gcggcggtct gccgctgtcc gcggcggtcg
     1741 gccccgcgga aattctcgac gagcctcccg cggcagcgct gctg
//
LOCUS       DASYLX010000073         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_552436,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000073 DASYLX010000000
VERSION     DASYLX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552436"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGP27_01810"
     CDS             <1..492
                     /locus_tag="VGP27_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850083.1"
                     /translation="VRVPAENLLGERGRGYANFLRILDEGRIAIAALSVGAAQGCVDE
                     CVKYAKEREAFGAKIGTYQAVAFKIARMEARAHVARTAYYDAAALMLSGKPFKKAAAI
                     AKLVASEAAMDNSRDATQVFGGYGFMNEYPVARHYRDSKILEIGEGTTEVQLMLIARE
                     SGL"
     gene            516..998
                     /locus_tag="VGP27_01815"
     CDS             516..998
                     /locus_tag="VGP27_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7850084.1"
                     /translation="MTDKRVVVQRGLWFEEFEPGVLYQHRPGRTITEADNVLFTTLTM
                     NTQALHLDAAFSDALPPFHQRLVNSMFTLSTLVGLSVAQLTQGTIVGNLGFGEVAFPK
                     PLFHGDTLYAETEVTDKRESKSRPGEGIVTFAHTGRNQHGDIVATASRKTMVRKQPEE
                     "
     gene            1000..1818
                     /locus_tag="VGP27_01820"
     CDS             1000..1818
                     /locus_tag="VGP27_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV7850085.1"
                     /translation="MALAQTGPGWLFCPADRPERFGKAAAAADVVILDLEDGVAAKDR
                     DAARAALVETPLEPDRTVVRINPATTDDHAADLEALAKTKYTTVMLAKTEDPQQIRDL
                     APLNVVVLIETPLGAVVVNELARMDNAFALMWGAEDLFAVTGGTANRWPDGSYRDVAK
                     HVRSQTLLAAKAYGRMALDSVYLNIKDLDGLRGEVDDAVAVGFDAKVAIHPTQVAVIR
                     EGYAPTSDQVEWARHVLAAARDERGVFQFEGIMVDAPVLRRAERIVQLAPHPGT"
     gene            1919..3019
                     /gene="pdhA"
                     /locus_tag="VGP27_01825"
     CDS             1919..3019
                     /gene="pdhA"
                     /locus_tag="VGP27_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEV7850086.1"
                     /translation="MAGFVQAPLTSYNVELEPIQLVAPDGTPTTETRYRRDLPPETLA
                     WLYESMAVTRALDGEFVNLQRQGELALFASCRGQEAAQIGSAACLRKTDWLFPQYREI
                     GAFLLRAITPAQMGAVWRGRWHGGLGFTEKCVAPIAIPIGTHGLHAVGAAMAAQRLGE
                     DSVTVAYLGDGATSEGDAHEAMNLASVYQAPCVFFVQNNQWAISVPVSRQQAGPSIAH
                     RAIGYGMPGVRVDGNDVLACYAVTAQAAQRAREGRGPTLIEALTYRMGPHTTSDDPTR
                     YRPQDEVDRWAARDPISRYRSYLQAIGVWSERLEHRVETRAARLCAELRDATVNTADM
                     DIGELFDTVYADITPTLQAQRDQLMTELAKEA"
     gene            3077..4132
                     /locus_tag="VGP27_01830"
     CDS             3077..4132
                     /locus_tag="VGP27_01830"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV7850087.1"
                     /translation="MTQIIERPADYGDDAQEPSLFTPVRVTTNLTMVQAINRALHDAM
                     AADDRVLVFGEDVARLGGVFRVTEGLTETFGDERCFDTPLAESAIIGIAVGMAIRGFV
                     PVPEIQFDGFSYPAFDQVVSHLAKYRMRTHGDVDMPVTIRIPSFGGIGAVEHHSESTE
                     TYWAHTAGLKVVVPSTPSDAYWLLRYSIASRDPVVFLEPKRRYWSREEVDTNEPGLPI
                     GRAAVRCMGDDVTVLTYGGLVQTALAAADLAADRYGWNLEVVDLRSLNPLDFDTVAAS
                     VRKTGRAVVMHEGARTLGFGAELAARIQEELFYDLEAPVLRATGFDTPYPPARVEKLW
                     LPGVDRLIDCVEKAMHS"
     gene            4129..>4280
                     /locus_tag="VGP27_01835"
     CDS             4129..>4280
                     /locus_tag="VGP27_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV7850088.1"
                     /translation="MSGHDGSAPRARGGVRDFLVPDLGEGLEDATITSWSVAVGDDVA
                     LNQTLCT"
BASE COUNT          754 a         1365 c         1464 g          697 t
ORIGIN      
        1 gtccgggtgc ccgccgagaa cctgctcggc gagcgcggcc gtggctatgc caactttctg
       61 cgcattctcg acgagggccg catcgcgatc gccgcgctgt ccgtcggcgc cgcacagggc
      121 tgcgtcgatg aatgcgtgaa atacgcaaag gaacgcgagg cgttcggcgc gaagatcggt
      181 acctaccagg ccgtcgcctt caagattgcg cgcatggagg cccgggcgca tgtcgcacgc
      241 accgcctact acgacgcggc ggcactcatg ctgtcgggca agccgttcaa gaaggccgcc
      301 gccatcgcca agctggtggc cagcgaggcc gcgatggaca actcccgcga cgccacgcag
      361 gtgttcgggg gctacgggtt catgaacgaa tacccggtcg cgcggcacta ccgcgacagc
      421 aagatactcg aaatcggtga gggcacaacg gaagtgcaac tgatgctgat tgcgcgggag
      481 tccgggctgt gagcgaagtg aacgattcgg cagaagtgac cgacaagagg gtcgtcgtac
      541 agcggggcct gtggttcgag gagttcgagc ccggggtgct ctaccagcac cggcccgggc
      601 gcaccatcac cgaggcggac aacgtgctgt tcaccacgct cacgatgaac acccaggcgc
      661 tgcacctcga tgccgcgttc tccgatgcgt tgccgccgtt tcaccagcgg ctggtcaact
      721 cgatgttcac gttgtcgacg ctcgtcgggc tgtccgtggc gcagctgacg cagggcacga
      781 tcgtcggcaa cctcggcttc ggcgaggtcg cgttcccaaa gccgcttttc cacggcgaca
      841 ccctgtatgc cgagaccgag gtgacggaca agcgcgagtc gaagagccgt ccgggggagg
      901 gcatcgtcac attcgcccat accggccgca atcaacacgg cgatatcgtc gcgaccgcat
      961 cgcgaaagac gatggtgcgc aagcagcctg aggagtgaga tggctttagc tcagaccggc
     1021 cccggctggt tgttctgccc ggccgaccgc cccgaacgat tcgggaaggc agctgcggcg
     1081 gccgacgtgg tgatcctcga cctcgaagac ggtgtcgcgg ctaaagatag agacgccgcg
     1141 cgtgctgccc tcgtggaaac cccgttggaa cccgaccgca ccgtcgtcag aatcaacccc
     1201 gccacaaccg acgaccacgc ggcggatctc gaggcgctcg cgaagaccaa gtacaccacc
     1261 gtgatgctgg ccaagacgga ggacccgcag cagatccggg acctggcacc gctgaacgtc
     1321 gtcgtgctga tcgagacgcc gctgggcgca gtcgtcgtca acgaactggc ccgcatggac
     1381 aacgccttcg ccttgatgtg gggcgccgag gatctgttcg cggtgacggg tggcaccgcc
     1441 aatcggtggc ccgacgggag ctaccgcgac gtcgccaagc atgtccgctc gcagacgttg
     1501 ctggcggcga aggcgtacgg gcggatggcg cttgattcgg tgtacctcaa catcaaggat
     1561 ctcgacggtt tgcgcggcga ggtcgatgac gccgtcgcgg tcggcttcga cgccaaggtg
     1621 gcgattcatc cgacgcaggt cgcggtcatc cgcgagggtt acgcacccac gtccgaccag
     1681 gtcgaatggg cgcgacacgt gctcgccgcg gcccgggatg aacgcggcgt ctttcaattc
     1741 gagggcataa tggtggatgc cccagtgctg cggcgagcag agcgcatcgt ccagctagcg
     1801 ccacaccctg gcacctagcc ggcgacctct taccaggggt tttgctctga ccgcatcagt
     1861 gctgagcacc tcagtcgcct gacggcgcga tccgccgtca gtgaccctag gaggcggtat
     1921 ggcagggttt gttcaggcac ccctgacgtc gtacaacgtc gagctggagc cgattcaact
     1981 ggtcgccccg gacgggacgc cgaccaccga gacccggtat cgccgagatc ttccacccga
     2041 gacgctggcc tggctgtacg agtcgatggc cgtcacccgc gcgctcgacg gggagttcgt
     2101 caacctgcag cggcagggcg agctcgcgct gttcgcgtca tgtcgcggtc aggaagccgc
     2161 acagatcggc tcggccgcgt gcctgcgcaa gaccgactgg ctgttcccgc agtatcgaga
     2221 gatcggcgcg ttcctgctgc gcgccatcac acccgcgcag atgggcgcgg tctggcgcgg
     2281 ccggtggcac ggcgggctcg ggttcaccga gaagtgcgtc gcgccgatcg cgatcccgat
     2341 cggcacccat ggtttgcacg cggtcggtgc ggcgatggcc gcgcagcgcc tcggcgaaga
     2401 ctctgtcacg gtcgcctacc tcggcgacgg agcgaccagc gagggtgacg cacacgaagc
     2461 gatgaatctt gcttcggtgt accaggcgcc gtgcgtgttc ttcgtgcaga acaaccagtg
     2521 ggcgatctcg gttccggtga gccgccagca ggcgggtccg tcgatcgcgc accgggcgat
     2581 cggctacggc atgcccgggg tccgggtgga cggtaacgat gtgctggcgt gttatgcggt
     2641 gacggcccag gccgcgcaac gcgcccgtga gggtcgcggt cccacgctga tcgaggcgct
     2701 gacgtaccgg atggggccgc ataccacctc tgatgacccg acgcgttacc gaccgcagga
     2761 cgaggtagac aggtgggccg cgcgggaccc gatttcgcgt taccgcagct atttacaggc
     2821 tattggcgtc tggtccgaac gtctggagca ccgagtcgag acacgggcgg cgcggctgtg
     2881 cgcggaattg cgtgacgcaa ccgtcaacac tgcggacatg gacatcggag aactcttcga
     2941 caccgtctat gcggacatca cgccgacgtt gcaagcccag cgcgaccaac tgatgaccga
     3001 gctggcgaag gaggcgtgac atgacactgc gcacgcgtgg gggtacctcc cgccagaagg
     3061 gtagggggag agcgaaatga cccagatcat cgagcggccc gccgattacg gggatgacgc
     3121 gcaggagccg tcgctgttca cgccggtgcg ggtaacgacg aatttgacca tggtgcaggc
     3181 gatcaatcgg gctcttcacg acgcgatggc cgccgacgac cgtgtcctgg tcttcggcga
     3241 ggacgtggcg aggctcgggg gtgtgttccg ggtgaccgag ggcttgaccg aaacattcgg
     3301 cgatgagcgg tgtttcgaca cacccctggc ggaatcggcg atcatcggta ttgcggtcgg
     3361 gatggcgatc cgcggcttcg tcccggttcc cgagatccag ttcgacgggt tctcgtatcc
     3421 ggcgttcgac caggtggtca gccacttggc gaagtaccgg atgcgcaccc acggcgacgt
     3481 ggacatgccg gtcaccatcc gcattccgtc gttcggcgga attggcgcgg tggaacacca
     3541 ctctgagtcc accgagacgt attgggcgca caccgccggc ctgaaagtcg ttgtgccgtc
     3601 gacgccctca gatgcctact ggttgctgcg gtattcgatc gccagcaggg acccggtcgt
     3661 gttcctcgag ccgaagcggc ggtactggtc gcgcgaagag gtcgacacca atgagcccgg
     3721 acttcccatc ggccgggccg cggtgcggtg catgggcgat gacgtcacgg tgctgaccta
     3781 cggcggcttg gtccaaacag ccttggccgc agcggatctg gcggcggacc gatacggatg
     3841 gaatctggaa gtcgtcgacc tgcgatcgct caacccgctc gacttcgaca ccgtcgcagc
     3901 gtcggtgcgc aagaccggac gcgcggtggt catgcacgaa ggggcccgca cactcggctt
     3961 cggcgccgag ttggcggcgc ggatccagga agagctgttc tacgacctgg aggcgccggt
     4021 gttgcgcgcc accggattcg acacccccta cccgccggct cgggtggaaa agctgtggct
     4081 acccggcgtc gaccggctga tcgactgcgt cgagaaggcg atgcactcat gagcggccac
     4141 gatggttccg ctcctcgcgc gcgcggcggt gtgcgcgact tcctggtacc cgatctcggc
     4201 gagggcctcg aggacgcgac gatcaccagc tggagcgtcg cggtcggcga cgacgtcgca
     4261 ctcaatcaaa cgctgtgcac
//
LOCUS       DASYLX010000074         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2088560,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000074 DASYLX010000000
VERSION     DASYLX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088560"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VGP27_01840"
     CDS             complement(<1..974)
                     /locus_tag="VGP27_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEV7850089.1"
                     /translation="MKVAAGLRARERVVVHVDSQAARWISALAVLSLLGWLILLHKET
                     SWSMTILTAVVLIARGIFLGRPVTAGHATAAMVVCIAGTAAVFLAFDLLGNLLLVASA
                     VVLMSPTSARPNPADLPRVFALVNATTGDPLAPFAMQASKCYHFSADGGAAIAYRTRL
                     GFAVVSGDPIGEDAQFAALVADFAAMCGTRGWRIAVLGCSRQRVDLWSNPAVLPRKLT
                     PVPIGRDVIIDVATFDMVGRKYRNLRQAVQRTKNVGVTTEVVDEQALNDILFAELTDV
                     IRASPSGARTERGFSMSLDGTLEGRYPGVALIIARDKAGRVQGFHRYAT"
     gene            complement(974..1852)
                     /locus_tag="VGP27_01845"
     CDS             complement(974..1852)
                     /locus_tag="VGP27_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEV7850090.1"
                     /translation="MLLSDFEELLAVLDLNRVDDGSFDGMFVGSHPSKNPPRTFGGQM
                     LAQAFVAASRTLDRDLPPGAVSAHFIAGGDPAKDLEFHVVRLRDERRFANRRVDVVQD
                     GELLTTAIISFVDGGRGLEHGIECPDVPDPLSLPKIDDLLVGYEKTVPHFVEALRPIE
                     WRYTNDPAWVMRNKGERLGHNRVWLKADGPMPEDPVLHSAALIYSSDTTVLDSIITTH
                     GLSWGYDRIFAATVNHSVWFHRPFRFDEWVLYSTSSPVAADSRGLGTGHFFDQSGQIV
                     ATVVQEGIIKYFPEAK"
     gene            complement(1956..3374)
                     /gene="pyk"
                     /locus_tag="VGP27_01850"
     CDS             complement(1956..3374)
                     /gene="pyk"
                     /locus_tag="VGP27_01850"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV7850091.1"
                     /translation="MTRRAKIVCTLGPATSEDHVVRALVEAGMDVARLNFSHGEYGDH
                     EAHYKRVRAASDTTGRAVGILADLQGPKIRIGRFATGPTYWANGETVRITVDDIDGTH
                     DRVSTTYKQLAKDAAPGDRLLVDDGNVGLVVEHIDGNDVVCTVTEGGPVSNNKGLSLP
                     GMNVSVPALSEKDIDDLEFALRLGVDLVALSFVRSPADAELVHEVMDRVGRRVPVIAK
                     LEKPEAVDNLEAIVLAFDAIMVARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVAT
                     QMLESMIEHSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPLEAVRTMARIICAVE
                     DNSVVVPPLTHVPRTKRGVISYAARDIGERLDAKALVAYTQSGDTVRRLARLHTPLPL
                     LAFTSLPEVRSQLAMSWGTETFIVPHIQTTDGMIRQVDKSLLELGRYKRGDLVVIIAG
                     APPGTVGSTNLIHVHRIGEDDV"
     gene            complement(3406..4908)
                     /locus_tag="VGP27_01855"
     CDS             complement(3406..4908)
                     /locus_tag="VGP27_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEV7850092.1"
                     /translation="MADPHGFLEVPKVEAAKRPVDERVGDWREVYERQDPQSRAGEVS
                     QQARRCMDCGVPFCHSGTAGCPLGNLIPEWNDLVRRGRWDAASDRLHATNNFPEFTGR
                     LCPAPCEAACVLSIADDRTGGSVTIKRIEQTIADHAWMDGVVEPQPAAISTGKSVAVV
                     GSGPAGLAAAQQLTRAGHDVTVYERDDRLGGLMRYGIPEYKLEKATLNQRLAQMRAEG
                     TRFVTECEVGVDLSVEQLRERHDAVVLAVGALRGRDNDVEGRHLDGVHLAMEHLVPAN
                     RECEGDGPSHLSAQGRHVVIIGGGDTGADCLGTAHRQGAASVTQLDYNPEPPEYRDES
                     RSPWPMWPMVLRTSPAHAEGGARRYEVAVQRFVGDDRGHVRAIEIAEVQVTRDADGCR
                     LITPVGGSLQIPCDLALLAIGFDGVEHMALLDGLGLQLNGRGSLSCGSDWQTTAPGVF
                     VCGDAHRGASLIVWAIAEGRSAAHAVDAYLMGESDLPSPVMPGALPLAVA"
BASE COUNT          821 a         1711 c         1632 g          847 t
ORIGIN      
        1 gtggcgtagc ggtggaaccc ctgcacgcgg ccggctttgt cccgcgcgat gatcagcgcg
       61 acaccggggt acctgccctc gagtgtcccg tcgagcgaca tcgagaaccc gcgctccgtg
      121 cgggcaccgc tcggcgacgc gcggatcaca tcggtgagtt cggcgaacag aatgtcattg
      181 agggcttgct cgtcgacgac ttcggtggtg acgccgacgt ttttggtgcg ctgcacggcc
      241 tggcggagat tgcggtactt ccgcccgacc atgtcgaacg tcgccacgtc gatgatcacg
      301 tcgcgcccga tgggcacagg ggtcagcttc cggggaagca cagccgggtt cgaccacagg
      361 tccactcgct gtcggctgca gcccagcacg gcgatgcgcc agcctctggt gccgcacatc
      421 gcagcgaaat cggcgaccag cgcggcgaat tgggcgtcct caccgatcgg gtcgccgctg
      481 accaccgcga aaccgagccg ggtccgatag gcgatcgcgg cgccgccgtc ggcgctgaag
      541 tggtagcact tcgacgcctg catcgcgaac ggcgccagcg gatcgccagt cgtcgcgttc
      601 accaacgcga agacccgcgg cagatcggcc ggattcgggc gggccgaggt cggcgacatc
      661 agcaccaccg ccgaagccac cagcagcaag ttgccgagca aatcgaaggc gagaaacacc
      721 gccgcggttc ccgcaatgca caccaccatc gccgcggtgg cgtgccccgc tgtgaccggc
      781 ctgccgagga agatgccccg cgcgatcagc acgacggccg tcaggatggt cattgaccag
      841 gacgtttctt tgtgcagtag gatcagccag ccgagcaggc tgagcactgc gagcgcactg
      901 atccagcgag ctgcctgcga atcgacatgg acgacgacac gctctcgagc ccgaagaccg
      961 gctgctacct tcatcacttt gcttccggga agtacttgat gattccttcc tgaaccacgg
     1021 tagccacgat ttggcccgat tggtcgaaga aatgccccgt tcccaagccg cgtgaatcgg
     1081 ccgcgacggg cgaagaggtc gaatacagaa cccactcgtc gaaccggaat ggccggtgaa
     1141 accacaccga gtggttgacg gtggcagcga agatccggtc gtagccccac gacaggccgt
     1201 gggtggtgat gatcgagtcg agcaccgtgg tgtccgaaga gtagatcagc gccgccgaat
     1261 gcagcaccgg gtcttccggc atcggcccgt cggccttgag ccacacccgg ttgtgaccga
     1321 gccgttctcc cttgttgcgc atcacccagg ccgggtcgtt ggtgtaacgc cattcgatcg
     1381 gcctgagtgc ctccacgaag tgcggcaccg tcttctcgta gcccacgagg aggtcgtcga
     1441 tcttgggcag tgacaacgga tccggtacgt ccgggcactc gatgccgtgc tcgagtccgc
     1501 gcccgccgtc aacgaacgag atgatcgccg tggtcagcag ttcgccgtcc tgcacgacgt
     1561 cgacgcggcg gttggcgaag cgacgttcgt cgcgcagccg cacgacgtgg aactcgagat
     1621 ccttcgccgg gtcgccgccg gcgatgaagt gtgcggaaac cgcgccgggc ggcaggtcgc
     1681 ggtccagggt tcggctggcc gccacgaacg cctgcgccaa catctggccg ccgaatgtgc
     1741 ggggtgggtt cttactcgga tgcgacccga cgaacatgcc gtcgaacgag ccgtcgtcga
     1801 cgcggttgag gtcgagtacc gccagcaact cctcgaaatc gctcagcagc actgctcccc
     1861 ctacctagag cgatggaggt acccccaccc ctacgcttcg catgggaggt acccccacaa
     1921 cgcggctcgt tcctcggcgc ctgatcgtcg tccgactaaa cgtcgtcctc cccgatccgg
     1981 tgcacgtgaa tcaggttggt cgagcctact gtgcccggtg gggcgcctgc gatgatgacc
     2041 accaggtcgc cacgcttgta gcgacccaac tcgagcaggg atttatcgac ctgtcggatc
     2101 attccgtcgg tggtctgaat gtgcggcacg atgaacgtct cggtgcccca gctcattgcc
     2161 aattggctgc gcacctccgg caacgacgtg aacgccagca gcggtagcgg cgtgtgcagc
     2221 cgcgccagcc tgcgcaccgt gtcaccggac tgggtgtagg cgaccagcgc cttggcatcc
     2281 agccgctcgc cgatgtcgcg ggctgcatac gaaatgacgc cacgcttggt ccgtggcacg
     2341 tgcgtcagcg gcggcaccac caccgagttg tcctccaccg cgcagatgat gcgggccatg
     2401 gtcctgaccg cctcgagcgg atacttgccg actgacgtct cgccggacag catcaccgca
     2461 tccgcaccgt cgagcaccgc gttggcgacg tcagacgcct ccgcgcgggt cggccgggag
     2521 tgttcgatca tcgactccaa catctgggtg gcgacgatga cgggtttcgc attctccctt
     2581 gccatttgga tggcccgctt ctgaacgagc ggcacctctt ccaggggcag ttcgacgccg
     2641 aggtcgccgc gggccaccat gatcgcgtcg aacgccagca caatggcctc gaggttgtcg
     2701 acggcctcgg gtttctccag cttggcgatg acgggcaccc ggcggccgac ccggtccatc
     2761 acctcatgca ccagctcggc gtcggcgggt gatcgcacga aggacagcgc cacgaggtcc
     2821 actcccagcc ggagagcgaa ctcgaggtca tcgatgtcct tctccgacag cgcgggcacc
     2881 gacacgttca tgccgggcag cgagagtccc ttgttgttgc tgaccggccc gccctcggtg
     2941 actgtgcaca ccacgtcgtt gccgtcgatg tgctcgacga ccagaccgac gttgccgtcg
     3001 tccaccagca gccggtcccc cggtgcggcg tctttggcga gctgtttgta ggtcgtggac
     3061 acccggtcgt gagtgccgtc gatgtcgtcg acggtgatgc ggacggtttc gccgttcgcc
     3121 cagtaggtgg gccctgtcgc gaaccgcccg atgcggatct tgggaccttg caggtcggcc
     3181 aggatgccga ccgcccggcc cgtggtgtcc gacgccgcgc gcacccgctt gtaatgcgcc
     3241 tcgtgatcgc cgtactcgcc gtggctgaag ttgagtcgag ccacgtccat tccggcttcg
     3301 accagcgccc tgacgacgtg gtcctcactg gtggcagggc caagagtgca gacgatcttt
     3361 gcgcgtctag tcacgacttc tgagcatagt cctgatcgga gtccgtcacg cgacggcgag
     3421 gggcagtgcg ccgggcatca ccggcgacgg caggtctgat tcgcccatca gatatgcgtc
     3481 gaccgcatgc gctgcgctgc gcccttccgc aatcgcccag acgatcagcg acgccccgcg
     3541 atgggcatct ccgcagacga atacgccggg ggcggtcgtc tgccagtccg aaccgcacga
     3601 caacgaccca cgaccgttca gttgcagccc gagcccgtcg agcagcgcca tgtgctcgac
     3661 accgtcgaac ccgatcgcca gcagggcgag gtcgcagggg atctgcaaag atcctccgac
     3721 cggggtgatg aggcgacagc cgtcggcgtc acgggtgacc tgcacctccg cgatctcgat
     3781 cgcgcgaaca tggcctcggt cgtcgccgac gaatcgctga acagcgacct cgtagcgacg
     3841 cgctccgccc tccgcgtgcg caggagatgt gcgcagcacc atcggccaca tcggccacgg
     3901 cgatcgcgat tcgtcgcggt actccggcgg ctccgggttg tagtcgagct gggtgaccga
     3961 cgcggcgccc tggcggtgcg cggtacccag gcagtccgcg ccggtatcac cgccaccgat
     4021 gatgacgacg tgcctgccct gggccgacag atgtgacggg ccgtcgcctt cgcactccct
     4081 gttggccggc acgagatgtt ccatcgcgag gtgcacgccg tcgaggtggc gcccctcgac
     4141 gtcgttgtcc cggccgcgca gcgccccgac ggccagcacc accgcgtcgt gccgctcgcg
     4201 cagctgctcg accgagaggt cgacgccgac ttcacattcg gtgacgaaac gggttccctc
     4261 cgcccgcatt tgggccaggc gctgattcag cgtcgccttc tcgagtttgt actcggggat
     4321 cccgtagcgc atcaggccgc ccagccggtc gtcgcgctcg tagaccgtga cgtcgtgacc
     4381 ggcgcgcgtg agttgttgcg cggctgccag tccggcgggg ccggagccga ccacggcgac
     4441 gctcttaccc gtcgagatgg cagcgggctg cggttcgacc acaccatcca tccacgcgtg
     4501 gtcggcgatg gtctgctcga tgcgtttgat ggtgacgctg ccaccggtcc tgtcatctgc
     4561 gatggacagc acgcaggcgg cttcgcacgg ggcaggacac aggcggccgg tgaactcggg
     4621 gaagttgttg gtggcgtgca gtcggtcgct tgcggcatcc caccggccgc ggcgaaccag
     4681 gtcgttccac tccgggatca ggtttcccaa cgggcaaccg gcagttcccg agtggcagaa
     4741 cgggaccccg cagtccatgc accgacgcgc ctgctgggac acctcgccgg cccgggactg
     4801 cggatcctgc cgttcataga cttcgcgcca gtccccgacg cgctcgtcga cgggccgctt
     4861 cgccgcctcg accttcggca cctccaggaa gccatgcgga tcagccacgg ctggcctcca
     4921 tgatcgcggt gtcgacgtcg cggccctcgg ccttggccat ccgggttgcg gtcagcacac
     4981 gctggtagtc ggtcggcatg accttggtga a
//
LOCUS       DASYLX010000075         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2308142,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000075 DASYLX010000000
VERSION     DASYLX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308142"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1882)
                     /locus_tag="VGP27_01860"
     CDS             complement(<1..>1882)
                     /locus_tag="VGP27_01860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV7850093.1"
                     /translation="LLLVDDAQHLDPQAARLVRVLAAGAELTIVAGDPNQSVFSYRGA
                     DPALLRGTDDDPVITLTESHRCTPAVARAITGVARRLPGAVEARELVGAGDDLGTVSV
                     RLAASPHAEAAMIADALRRAHLFDGVPWSQMAVIVRSLRQSGAPLSRALTAAGVPVIN
                     PPSGVPVAEQAASRAMLTVLQAVADGLDGDRAMQLLTGPIGRVDPVSLRQLRRALRRA
                     DRSGPPRDFGELLVEAMESQKLSVTGDLARPVQRVRAVLAAARRGEAGGADPRHTLWQ
                     AWHRSGLQKRWLAASERGGAAGANADADLDAVTALFDVTEQYVSRTAGSSLRGLVEQV
                     RALGPLPTAGDPPSDTDGVTLVSAHAALGKEWDVVVIAGLQEGLWPNTTPRGGVLATQ
                     FLVDVLDGVVADTAGVSSRAPLLAEERRLLIAAMGRARNRLLVTAVDSECGNDAMLPS
                     PFHVELAQFATDADTATIEPVPAPRVLAPAPLVGGLRAVVCAPEGAVTDPVRICAAAQ
                     LARLAAAGISGADPGQWYGMTAPSTDEPLWAGDDQVVRLSPSTLQTLADCPLRWLLER
                     HGGSDGRDVRSAIGSLVHALVADPEMREGQMVAELERLWEKLPFESAWYADNELARHR
                     LML"
BASE COUNT          279 a          701 c          653 g          249 t
ORIGIN      
        1 cagcatcaag cggtgccggg ccagctcgtt gtcggcgtac cacgccgact cgaacggcag
       61 cttctcccag agcctctcga gctcggcgac catctgaccc tctcgcattt ccggatccgc
      121 gaccagggcg tgcaccaacg agccgatggc cgagcgcacg tcgcgtccgt cgctgccgcc
      181 atgccgttcg agcagccagc gcaacgggca gtcggccagc gtctgcagtg tcgacggcga
      241 cagccgcacc acctggtcgt cgcccgccca cagcggctca tcggtgctcg gcgcggtcat
      301 gccgtaccac tgtccgggat cggcgccgga tatgcctgcg gcagccaggc gcgccaattg
      361 tgctgccgca cagatcctta ccggatcggt gacggcgccc tcgggcgcgc ataccaccgc
      421 gcgcagcccg ccgaccagcg gtgcgggcgc cagtacccgc ggggcgggca ccggctcgat
      481 ggtggccgta tcggcgtcgg tcgcgaactg ggccagctcg acgtggaacg gcgatggcag
      541 catcgcgtcg ttaccacatt cgctgtccac cgctgtcacc agcagcctgt tgcgggcccg
      601 gcccatcgcg gcgatcagca gcctgcgctc ctcggccagt agcggcgccc ggctggacac
      661 gcccgcggtg tcggcgacga caccgtcgag gacatcgacg agaaactggg tcgcgagaac
      721 accgccgcgg ggagtggtgt tgggccacag cccttcctgc aggcctgcga tgacgacgac
      781 atcccactcc ttacccagcg cggcatgcgc gctcaccagc gtgacgccgt cggtgtcgga
      841 cggcggatcg cccgccgtcg gcaacggacc cagcgcgcgc acttgctcga cgaggccgcg
      901 aagggacgac ccggcggttc tcgacacgta ctgctcggtt acgtcgaaca gcgcagtgac
      961 cgcgtccagg tcggcgtcgg cgttcgcgcc cgccgcccca ccccgctcgc ttgccgcaag
     1021 ccatcgtttc tggagaccgg agcggtgcca cgcctgccac agggtgtggc gcgggtctgc
     1081 gccgccggcc tcgccgcgcc gcgcggcggc cagcacggcg cgcacccgct gcaccggacg
     1141 cgcaagatca cctgtgacgg agagcttttg cgattccatc gcctcgacga gcaattcgcc
     1201 gaagtcgcgc gggggcccgc ttcgatccgc tcggcgcagc gctctgcgaa gttgccgcaa
     1261 cgacaccgga tcgacccggc cgatcggccc ggtgagcagt tgcatggcgc ggtcgccgtc
     1321 cagcccgtcg gcgacggcct gcaacaccgt cagcatcgcc cgcgatgcgg cctgttcggc
     1381 gaccggcaca cccgacggcg ggttgatcac cggaaccccc gcggcggtga gtgcgcggga
     1441 cagcggcgcg ccgctctgcc gcagcgagcg cacgatcacg gccatctgcg accacggcac
     1501 cccgtcgaag agatgggccc tgcgcagcgc gtcggcgatc atcgcggcct cggcgtgcgg
     1561 tgacgccgcc agccgcaccg acactgtgcc cagatcgtcg ccggcgccga caagttcgcg
     1621 ggcctcgacg gcgccgggca gccgccgcgc gacgccggtg atggcgcggg ccaccgcggg
     1681 cgtgcagcgg tgcgactccg tcaaggtgat caccggatcg tcgtcggtac cccgcaacag
     1741 cgccggatcg gcgccacggt aggagaacac ggattggttg gggtcaccgg cgacgatggt
     1801 cagctcggcg ccggccgcaa gcacccgcac caggcgggcg gcctgagggt cgaggtgctg
     1861 ggcgtcgtcg accagaagca gc
//
LOCUS       DASYLX010000076         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2088690,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000076 DASYLX010000000
VERSION     DASYLX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088690"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGP27_01865"
     CDS             complement(<1..569)
                     /locus_tag="VGP27_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850094.1"
                     /translation="MDFRDSPDEAAFRERLRGWLTEQKGRFPTSGDDYWAKAGEWHVA
                     LYEAGFFGTSWPAAYGGQDLPPVYDVIVDEEIAKAGAPARPSLGYLVVGLSHHGNEEL
                     RQRFLPGMINGTERWCQGFSEPGAGSDLASLTTTAVRDGDEYVIAGHKIWTSYSDVAD
                     WCLVLARTDKDVPKHKGISAFVVSMHQPG"
     gene            complement(556..1368)
                     /locus_tag="VGP27_01870"
     CDS             complement(556..1368)
                     /locus_tag="VGP27_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850095.1"
                     /translation="MDVRLTAEQRQLREAAAEVADDLGPGSVADLDDAGRVARLEKAI
                     DGTGFRTLRSDGASGVEVAIVAEEFARGLVDVPFIGPMLADDLGRRVGQPLSSAPEAP
                     SVDFTRSLAGVDESPTELGQLSEEDAGRWYALALTATTADIVGAARGTLTLAAEYAKV
                     REQYGATIGSYQAVGHLLAESLALIEGCVSVSRHAAWAVDELPVVEAVEAGRVAKVYC
                     ARSALTVCETSIQVHGGIGNTWECLAHVYLRRVLAATEAWPVKLEELTIGLS"
     gene            complement(1346..2749)
                     /locus_tag="VGP27_01875"
     CDS             complement(1346..2749)
                     /locus_tag="VGP27_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850096.1"
                     /translation="MSEPAALAFDEREYTLAELDSLANGMATTLEHRGVRTGDRVALM
                     SSNRPEFVIALRAIWRLGAAAVLLSPAWRETEVEHALALTKPSHAVGDHPVLAGKMSM
                     LSLDDPVTAGRCSFDPPPADSDALFVFSSGTTGMPKAVRHTHGAFAVAMRHWREALGL
                     TSIDRMQIVTPPSHILGLLNIAMALDTGAWIRLHRRFDIDQMLHHIEADRITIEMAVA
                     PIALALAAHPKLDSYDLSSLRYVMWCATPVTESVAEAVTATAGVNWVTAYGTSEMPVI
                     SCNSLQGARLDTVGRPVTGVDVRVVSLDDGRPAGPGEVGEIQAQSDSVMAGYLPDEAT
                     AQAFSDGWYRTGDVGYLDCDGWLRITDRAKEMIKVRGFQVAPAEIEAVLHGHPGVDDC
                     AVFGVDDVADGEAVVAAVATHGSVDPGELVALVADRLASYKQLSRVVVVPEIPRLPSG
                     KVLRRVLKGQYGRPSDG"
BASE COUNT          500 a         1042 c          933 g          463 t
ORIGIN      
        1 atgcctggct gatgcatcga cacgacgaac gccgagatgc ccttgtgctt gggcacgtcc
       61 ttgtccgtgc gagccagcac cagacaccag tcggcgacat cggagtaact cgtccagatc
      121 ttatggccgg cgatgacgta ctcgtcaccg tcgcggactg cggtcgtcgt caacgaagcg
      181 agatccgaac ccgcacccgg ctcgctgaag ccctgacacc accgctccgt cccgttgatc
      241 atgccgggca ggaaccgctg ccgcagttct tcattcccgt gatggctcag tccgacgacg
      301 aggtagccca ggctgggccg cgcaggcgca ccggccttcg cgatctcctc gtcgacgatc
      361 acgtcataca ccggcggcag atcctgaccc ccgtaggccg cgggccacga cgtgccgaag
      421 aaaccggcct cgtacaacgc aacgtgccat tccccggcct tggcccagta gtcgtcaccc
      481 gaggtgggga acctgccctt ctgctcggtc agccacccgc gcaaccgctc ccggaaggcc
      541 gcctcgtccg gtgaatcacg aaagtccaat ggtcaactcc tccagcttca ccggccacgc
      601 ctcagtggcg gcgagcaccc ggcgcaggta gacgtgcgcg agacactccc aggtgttgcc
      661 gataccgccg tgcacctgaa tggacgtctc gcaaaccgtc aatgccgaac gcgcgcagta
      721 aaccttggcc actcggcctg cctccacggc ctcgacgacg ggcaattcgt cgaccgccca
      781 cgcggcgtgc cgcgacacgc tgacgcatcc ctcgatcagc gccagactct cggccagcag
      841 gtggccgacg gcctgatagg aaccgatcgt cgcgccgtat tgttcgcgca cctttgcgta
      901 ctcggcggcc agtgtcaggg tgccgcgcgc ggcaccgacg atatcggccg tcgtcgcggt
      961 gagtgcaagt gcgtaccagc ggccggcgtc ctcctccgac aactgtccga gctcggtcgg
     1021 cgactcatcc actccggcaa ggcttctcgt gaaatcgaca gacggggcct cgggtgccga
     1081 cgacagcggt tgcccgaccc ggcggccaag atcgtctgcc agcatcgggc cgatgaacgg
     1141 gacgtccacc agacccctgg cgaactcctc ggcgacgatg gccacctcga cgccggacgc
     1201 cccgtcggag cgcagggtgc gaaaccccgt cccgtcgatg gctttttcca gccgtgcgac
     1261 gcggccggcg tcgtcgaggt cggcgaccga ccccggtccg aggtcgtcgg ccacctcggc
     1321 ggcggcctcg cgcagctgcc gctgctcagc cgtcagacgg acgtccatac tgtcccttca
     1381 gcactcggcg caatactttt ccagaaggca atcgcggaat ctcgggaacc accaccaccc
     1441 ggctcaattg tttgtacgac gccagccggt cggcgacgag ggcgacgagt tcgccgggat
     1501 ccaccgagcc gtgcgtggcg accgcggcga cgacggcttc gccgtccgcg acatcgtcga
     1561 cgccgaagac cgcgcagtcg tcgacccccg ggtgtccgtg cagcaccgcc tcgatctcag
     1621 cgggcgccac ctgaaatccc cgaaccttga tcatctcctt ggcgcggtcg gtgatacgta
     1681 gccagccgtc gcagtcgaga tagccgacgt cgccggtcct gtaccaaccg tcggaaaatg
     1741 cctgtgccgt agcttcatcg ggcaggtaac cggccatcac cgaatccgac tgcgcctgga
     1801 tctcgccgac ctcgcccggc cccgccggcc tgccgtcgtc gagcgacacc acccgcacgt
     1861 cgacgccggt caccgggcgt cccacggtgt ccaagcgcgc accctggagc gaattgcagg
     1921 agatcaccgg catttcgctg gtgccgtaag cggtcaccca gttcacgccg gccgtggcgg
     1981 tgacggcctc cgcgacgctc tcggtaaccg gcgtggcgca ccacatgacg taccgcagtg
     2041 acgacaggtc gtaggagtcc agcttcggat gggcggccaa cgccagcgcg atgggtgcga
     2101 cggccatctc gatggtgatc cggtccgcct cgatgtggtg cagcatctgg tcgatgtcga
     2161 agcgtcgatg caaccggatc caggccccgg tgtcgagcgc catggcgatg ttgagcaggc
     2221 cgaggatgtg cgacggcggc gtgacgatct gcatgcggtc gatcgacgtc aggccgaggg
     2281 cctcgcgcca gtgccgcatc gccaccgcga acgccccgtg cgtgtgccgc accgcctttg
     2341 gcatgccggt cgttccggag ctgaacacga acagcgcgtc ggaatcggcg ggcggagggt
     2401 cgaaggaaca ccgccctgcg gtgacggggt cgtccagcga cagcatcgac atcttgcctg
     2461 caagcaccgg atgatcgccg acggcgtgcg atggcttggt gagcgcgagc gcgtgttcga
     2521 cttcggtctc ccgccatgcc gggctgagca gcaccgccgc ggcgcccagc cgccagatgg
     2581 cccgcagcgc gatgacgaac tcgggccgat tcgacgacat cagcgcgacc cggtcgccgg
     2641 ttcgaacgcc ccggtgctcc agcgtcgtgg ccatgccgtt ggccagggag tcgagctcgg
     2701 ccaaggtgta ttcgcgctcg tcgaaggcga gggcggccgg ctcgctcacg tcagctcacc
     2761 cgcccttccg ccaccgtcgc ggtcgccttg cacgacgccg cgagaacaca ctatccttga
     2821 gtgagaatag tattctcgta gaagcagaat ctcaatggcg caaggagacg ctgaatggct
     2881 gtcgagggga ccataacgat cctgctcgac cggcagaagg cctcggtgcc gcgtgttg
//
LOCUS       DASYLX010000077         7154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1210831,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000077 DASYLX010000000
VERSION     DASYLX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210831"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VGP27_01880"
     CDS             <1..526
                     /locus_tag="VGP27_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7850097.1"
                     /translation="TNENGPKLMNSGKIAMLVTGPWDLSSLSDIDYGVQVMPTYAGSN
                     GGHQTIAGPDNWVVFDNGDKRKRAAIDFVKWLTAPEQVKAFSLGTGDLPIRASVGDDK
                     SVLDELNKNVPGTAEFVANLNNVKTVRPQVEQYPAVSEALGQAIVAVMLGKDQPAAAL
                     NNAAQAADAKLAEK"
     gene            531..1421
                     /locus_tag="VGP27_01885"
     CDS             531..1421
                     /locus_tag="VGP27_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7850098.1"
                     /translation="MPALLRRLSRTDSVSGWALVSPAVILIGIFGLLPVLMSLQLSFQ
                     KSDLLTPETPWVGFDNYRKLADDPVFVEAIKHTIIYTALFVPGTMLVGLFVAAALNRS
                     VRFISVYRTAAYITMAVSTISQGIIFLWLTDRDYGLVNAALNAVGAPSQPFLASPSQA
                     LYVIVAMTIWGWTGFSVIVYLAALQGVPAELHEAAAIDGASAFTRFRTITVPLLGPAN
                     LFLVVWLTINALQLFDEVYVTTRGGPLRATTVIVYYLWDRAFVQFDAGYAAAMAYALF
                     VVILVITAVQFRLARRHVHS"
     gene            1418..2326
                     /locus_tag="VGP27_01890"
     CDS             1418..2326
                     /locus_tag="VGP27_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7850099.1"
                     /translation="MTTTQLPPTETTAPQPESASRRRRLRLPFSPWHLVLIPVTFILI
                     VPLLWMLVTSLETEGEANRFPPVLFPESPRFANYSEAWAAAPFGHFFVNSALVTLVVL
                     VSNLVVCSLAGYAFARVRFLGRGALFVTLMATLMVPFQVTMIPVFLIVKWFGDNVWAG
                     LGIDHIGALMLPNLATAFGIFFLRQFFMTVPVELEEAARVDGTSRIGVLFKIILPLSL
                     PALSTLAALTVLTSWNDFLWPLIVITSQDQMTIPLGLSYFQGAHRVKWPLLMAANVMS
                     LLPMLLVFIGAQRYFVQSVASTGLKG"
     gene            2361..3467
                     /gene="ugpC"
                     /locus_tag="VGP27_01895"
     CDS             2361..3467
                     /gene="ugpC"
                     /locus_tag="VGP27_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV7850100.1"
                     /translation="MAEVEFRDVTRRYPGGVLALNDFNLTVSDGEFLILVGPSGCGKS
                     TALRLLAGLDKPTSGEIRIGSTVVNGLAPGQRDIAMVFQNYALYPHMSVYRNLAYGLR
                     QRKTPRAEVDRRVRETAKLLEIDQLLDRKPGQLSGGQRQRVAMGRALVRQPQAFLLDE
                     PLSNLDAKLRNQVRGDLKRLHREVPVTSIYVTHDQVEAMTLGHRLCVMAGGEVQQIGT
                     PDEIYNHPANTFVAAFMGSPPMNLIPADVRSGVLHVGSAVVTAVPSPDGPVTVGVRPE
                     HLRLHSRYAQGMVPARVDFVEPLGSHVLVTALVDGQNDPASGSAATRVVLQAPAGTDY
                     DAGTRIGLEMPPEATYFFDSDTGDVRRSQERIAL"
     gene            complement(3475..4953)
                     /locus_tag="VGP27_01900"
     CDS             complement(3475..4953)
                     /locus_tag="VGP27_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEV7850101.1"
                     /translation="MTSVQDGLVTRSPARLAADPSRVITRLFVPGQEGFEHQDSRAGA
                     VLRRILALDEDEVRSSLDDVVTRFDGRHRDLVGTFHRHAGELADRLDADIHRSLPRML
                     LLGATFTSEYAIEAAALCNPSVVAHPDQVGTAAGSLRFVMSVRGIGEGHRSSIGFRTG
                     VVDAQGGVTVDAPARFATVGTVGTTLLDAAVFRSELARLRDAGEAADYVLDALGDRFT
                     RADLDEQLDTLRTHMSTRGRAQQTISLIRAVAERFYAVAFADDIPLSERVLHPAMGAE
                     AAGMEDARFVRFVDDDGSVTFYATYTAYSGTHVSQQLLETADFQSFTSTPMAGNAASN
                     KGLALFPRRIRGRFAAMSRSDRESNSVAYADDPFIWPDSVPCQQPTQAWEALQLGNCG
                     PPIETEAGWLVLTHGVGPMRTYRIGAILLDLEEPTRIIGQLRRPLLSPAALEQDGYVP
                     NVVYSCGGLVHGEVLVIPYGIADAAIGIATVPLRELLAELGG"
     gene            complement(4950..5972)
                     /locus_tag="VGP27_01905"
     CDS             complement(4950..5972)
                     /locus_tag="VGP27_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7850102.1"
                     /translation="MTAASSPPKFDHLLRLTDSCGTFEHACFAEPRPEHGYCTDDMAR
                     VLVVATREPDADGPVKGLAGIAVRFLNEAQAYSGGCRNRMNRNGSWADEPTTGDHWGR
                     CLWGLGTAAAHSDVGIVQKLAVIQFGRAAQARSTSPRAMAYAALGAAELLAVDAEHAA
                     ARKLVTDYAVSLAAPNGDSSWPWPEPRLAYANAVLPEAMIAAGVALDAAALQQRGLDL
                     LGWLLDFESADGHLSPTPAAGRGIDDVRPAFDQQPIEVAALADACARAAAVDAAATWP
                     DGVAAAAAWFQGANDSGQVMWDSETGGGFDGLLADGVNQNQGAESTLAVISTLQQARR
                     FSVVPR"
     gene            complement(5969..7123)
                     /locus_tag="VGP27_01910"
     CDS             complement(5969..7123)
                     /locus_tag="VGP27_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7850103.1"
                     /translation="MLDPVEGQRFSRTARVGILSTYAPTHCGLATFTAALSDALCANG
                     ADVSVVRVADDGARSLSERVIGELVSGSARSVGASSELLNQNDVAVIQHEYGIYGGVD
                     GDDVVDVIDGLSVPSIVVAHTILKDPTPHQRSVLEAIAAMADHVVVMSEAARHRLCLG
                     FDVDRHKVTTIPHGAAVTSGPALKRPSRPTLLTWGLLGPGKGVERVIESMSSLKDLPG
                     RPRYLVAGRTHPKVLSGDGEAYRDARMEQARRSGVADSVTFDSRYWGASKLPALIQSA
                     AVVVLPYDSTDQVTSGVLVDAIACGRPVVATAFPHAVELLGTGAGIVVDHDDPDAMAS
                     ALRRVLTQPRLAGSMAAEARRLAPEMAWPVVASAYLSLADKLLADRRVLV"
BASE COUNT         1253 a         2375 c         2304 g         1222 t
ORIGIN      
        1 caccaacgag aacgggccga agctgatgaa cagcggcaaa atcgcgatgt tggtcaccgg
       61 cccgtgggat ctcagctcgc tgtccgacat cgactacggc gtgcaggtga tgcccaccta
      121 cgccggctcg aacggcggac atcagaccat cgccggcccc gacaactggg tggtgttcga
      181 caacggcgac aagaggaagc gggcggccat cgacttcgtc aagtggctga cggcgcccga
      241 acaggtcaag gcgttctcgc tgggcaccgg cgacctgccc atccgcgcgt ccgtcggtga
      301 tgacaagtcc gtcctggacg agctgaacaa gaatgtgccc ggcaccgcgg agttcgtcgc
      361 gaacctgaac aacgtcaaga ccgtcaggcc gcaggtggaa cagtatccgg cggtctcgga
      421 agcgctcggc caggcgatcg tggcggtcat gctcggcaag gaccagcctg cggccgccct
      481 taacaatgcc gcccaagccg ccgacgcgaa gctggccgag aagtaggccg atgcctgcat
      541 tgcttcggcg gctgagccgc accgacagtg tgtccgggtg ggcgctggtg agccccgcgg
      601 tcatcctgat cgggatcttc ggcctgctgc cggtgctcat gtcgctgcag ttgtcgtttc
      661 agaagtccga cctgctcaca ccggagacac cgtgggtcgg cttcgacaac taccggaagc
      721 tggccgacga cccggtcttc gtcgaggcga tcaagcacac catcatctat acggcgttgt
      781 tcgtgcccgg cacgatgctc gtcgggctgt tcgtcgccgc cgcactgaac cgctcggtgc
      841 gtttcatctc ggtctaccgc acagccgcct acatcaccat ggcggtgtcg accatctcgc
      901 agggcatcat cttcctgtgg ctgacagacc gggactacgg gctggtcaat gcggcgctca
      961 acgccgttgg cgcgccgtcg caaccgttcc tggcctcgcc gtcgcaagcg ctgtacgtca
     1021 tcgtggcgat gacgatctgg ggctggacag gtttttcggt catcgtgtat ctggctgcgc
     1081 tgcagggtgt tccggcggag ctgcacgagg cggccgcgat cgacggggcg agcgcgttca
     1141 cccgcttccg caccatcacc gtgccgctgc tcgggccggc gaacctgttc ctcgtcgtgt
     1201 ggctgacgat caatgcgctg cagctcttcg acgaggtgta cgtcaccacc aggggcggac
     1261 cactgcgtgc caccaccgtg atcgtctact acctgtggga tcgagcgttc gtgcaattcg
     1321 acgccggata cgccgcggcg atggcctacg cgttgttcgt cgtcatcctg gtgatcaccg
     1381 ccgtgcaatt ccggctcgcc cggaggcacg tgcactcatg acgaccacac agctgccgcc
     1441 caccgaaacg actgccccgc aacctgagtc ggcgagccgg cgccgtcggc tgcggctgcc
     1501 gttcagcccg tggcacctgg tgctgattcc ggtgacgttc atcttgatcg tgccgctgct
     1561 gtggatgctc gtcacctccc tggagaccga aggcgaggcg aaccgcttcc cgccggtgct
     1621 gttccccgag agtccgcggt tcgcgaacta ttccgaggcg tgggcggcgg cacccttcgg
     1681 gcacttcttc gtcaacagcg cgttggtgac gctggtggtg ctcgtcagca acctggtggt
     1741 ctgcagcctc gccggttacg ccttcgcgcg cgttcggttc ctcggtcgcg gagcgctttt
     1801 cgtgactttg atggcgacgc tgatggtgcc gttccaggtc acgatgattc cggtgttcct
     1861 catcgtcaag tggttcggcg acaacgtgtg ggcggggctg ggcatcgatc acatcggcgc
     1921 cctgatgctg ccgaacctgg cgacggcgtt cggcatcttc ttcctgcgcc agttcttcat
     1981 gacggtcccc gtggaactcg aggaggcggc ccgggtcgac gggacgtcac gcatcggtgt
     2041 gcttttcaag atcatactgc cgctgtcgct gcctgcgctg tccaccttgg cagcgctcac
     2101 cgttctcacg tcctggaacg acttcctgtg gccactgatc gtcatcacct cgcaggatca
     2161 gatgacgatc ccgttggggc tcagctactt ccagggcgcc caccgggtca agtggcccct
     2221 tctgatggcg gccaacgtca tgagcctgtt gccgatgctg ctcgtgttca tcggagccca
     2281 gcggtatttc gttcaatccg ttgcgagcac cgggctgaag ggctgatcgt ggcagccacg
     2341 gcgtcgagaa aggagttgcg ttggcggaag tagagtttcg tgacgtgacg cgccgctacc
     2401 cgggtggggt gctggcgctc aacgatttca acctgaccgt gtcggacgga gaattcctga
     2461 tcctcgtcgg accgtcgggg tgtgggaaga gcaccgccct gcgactgctc gcgggtctcg
     2521 acaagccgac gtcgggagag atcaggatcg gcagcaccgt cgtcaacggc cttgctcccg
     2581 ggcagcgcga catcgcgatg gtgttccaga actatgcgct gtatccgcac atgtcggtct
     2641 accgcaatct cgcctacggg cttcggcagc gcaagacacc gcgcgccgag gtggatcgac
     2701 gcgtacgcga gaccgccaag ctgctggaga tcgaccagct actcgaccgc aagcccggtc
     2761 agctctccgg tggccaacgc cagcgtgtcg ccatgggccg cgcgctcgtc cgtcagcccc
     2821 aggcgttcct gctcgacgaa ccgctgtcca atctcgacgc caaactgcgc aaccaggtcc
     2881 gcggggacct gaagcgtctg caccgtgaag ttcccgtcac ctccatctac gtcacccatg
     2941 accaggtcga ggcgatgacg ctgggccatc ggctgtgtgt gatggcgggc ggcgaggtgc
     3001 aacagatcgg aacccccgac gagatctaca accatccggc gaacacgttc gtcgcggcgt
     3061 tcatgggcag tccgccgatg aacctcattc cggcggacgt tcggtccgga gtcctgcacg
     3121 tcggcagcgc ggtcgtgacg gcggttccat ctccggacgg tccggtcacg gtcggcgtgc
     3181 gccccgagca tctgcgactg cacagccgct acgcccaggg catggtgcct gcgcgcgtcg
     3241 atttcgtgga accgctgggc agtcacgtct tggtgacggc gctggtggat gggcagaacg
     3301 acccggcgtc cgggtcagcg gcgacgcggg tggtgctgca ggcgccggca ggcaccgact
     3361 acgacgccgg caccagaatc ggtctcgaga tgccgccgga ggccacgtat ttcttcgact
     3421 ccgataccgg cgacgtccga cgcagtcagg agcgcatcgc gctctgagcg gctgtcagcc
     3481 gccgagctcg gcgagcagct cacgcagcgg cacggtcgcg atgccgatgg ccgcatcagc
     3541 gatcccgtag ggaatcacca acacctcgcc gtgcacgagt ccaccgcagg agtagacgac
     3601 gttcgggaca taaccgtcct gttcgagggc ggccgggctc agcagcggcc gccgcaattg
     3661 tccgatgatg cgggtgggct cctcgagatc cagcaggatc gccccgatcc ggtaggtacg
     3721 catcggaccg acgccgtgcg tgagcaccag ccacccagcc tcggtttcga tgggaggtcc
     3781 acagttaccg agctgcaacg cctcccaggc ttgcgtgggc tgctgacatg ggacggagtc
     3841 tggccagatg aacggatcgt cggcataggc aacagaattc gattcgcggt ctgaccgcga
     3901 catggcggca aagcgtccgc ggattcgacg cggaaagagt gccaggccct tgttggacgc
     3961 ggcgttccca gccatcggtg tggacgtgaa cgactggaaa tccgccgtct caaggagctg
     4021 ctggctgacg tgggttccgc tgtaggccgt ataggtggca tagaacgtga ccgacccgtc
     4081 gtcatcgacg aagcggacga agcgcgcgtc ctccataccc gccgcttcgg cgcccatcgc
     4141 cgggtgcagg acccgctcgg agagcggtat atcgtcggcg aatgcgacgg cgtaaaaccg
     4201 ctcggcaacg gcccgaatca gcgaaatcgt ctgctgcgca cggccgcggg tgctcatgtg
     4261 tgtccggagc gtgtcgagtt gttcgtcgag gtcggctctg gtgaacctgt cgcccagtgc
     4321 atcgagaacg tagtcggcag cctcgcccgc gtcgcgaagc cgcgccagct cactgcggaa
     4381 cacggcagcg tcgagcagcg tcgtgcctac cgttccgacg gtggcgaacc gggccggagc
     4441 gtcgaccgta acgccgccct gcgcgtcgac cacgcccgtt cgaaacccga tggatgaccg
     4501 gtgcccttcg ccgatacctc gaacgctcat cacgaatcgt aggctgcccg cggccgttcc
     4561 cacctgatcc ggatgggcga cgacgctcgg attgcacaat gccgcagcct cgatggcgta
     4621 ttcactggta aacgtggctc ccagcaacaa cattcgtggc agcgaccgat gaatgtcggc
     4681 atccaggcga tcggcgagtt caccggcatg ccggtggaag gtgccgacca ggtctcggtg
     4741 ccgcccgtcg aagcgcgtga cgacatcatc caacgatgac cgcacctcat cctcgtcgag
     4801 ggcaaggatg cgtctcagca cggctcccgc ccgggaatcc tggtgctcga aaccttcctg
     4861 cccggggaca aaaagtcggg tgatgaccct cgacgggtcg gcggcgaggc gcgccgggct
     4921 acgcgtgacg agcccgtcct gcaccgaagt catcgcggca caacggagaa acgccgagcc
     4981 tgctgcaatg tcgagatcac ggccagagtg gactcagcgc cctggttctg gttcaccccg
     5041 tcagcgagaa gtccatcgaa cccaccgccc gtctccgagt cccacatgac ctgccctgag
     5101 tcgttggcac cctggaacca agctgcagcg gccgcgacac catcgggcca ggtcgcagcg
     5161 gcgtcgacgg cggcggcacg cgcgcacgca tcggccaatg ccgccacctc gattggctgc
     5221 tggtcgaaag cgggcctgac atcgtcgatc cctctgccgg ccgccggggt cggagacaaa
     5281 tggccgtcag ccgattcgaa gtcgagcagc caacccaaaa gatccagccc acgctgctgc
     5341 aatgccgcgg cgtcaagcgc aactcccgca gcgatcatcg cctcgggaag caccgcgttc
     5401 gcgtaggcca gtcgcggctc aggccagggc caggagctgt cgccattagg cgcggcgagc
     5461 gacaccgcat agtcggtgac gagcttgcgc gcggcggcgt gctcggcgtc gacggcgaga
     5521 agctcagcgg ctcccagtgc ggcgtaagcc atcgcacgcg gcgatgtcga ccgtgcctgc
     5581 gcggcgcgcc cgaactggat caccgccaac ttctgaacaa tgccgacgtc actgtgagcg
     5641 gcggcggtac cgagccccca taaacacctg ccccaatggt cccccgtggt cggctcgtcg
     5701 gcccaactgc cgttgcggtt catccgattt cggcagccac cgctgtaggc ctgcgcctcg
     5761 ttcaggaaac gcaccgcgat cccggcgaga ccctttaccg ggccgtcggc gtcgggttct
     5821 cgggtggcca ccacaagaac gcgggccata tcgtcggtgc agtagccgtg ctcgggccgg
     5881 ggttcggcga agcacgcatg ctcgaaggtg ccgcaactgt cggtcaggcg cagcagatgg
     5941 tcgaacttgg gaggcgacga cgcggcggtc ataccagcac ccgccggtcg gcgaggagct
     6001 tgtccgccag actcaggtat gcgctcgcca ccaccggcca cgccatctcc ggcgccaatc
     6061 gacgggcctc cgcagccatc gagcccgcga gacgcggctg cgtcagcact cggcgcaggg
     6121 cagacgccat ggcgtccgga tcgtcgtggt cgacgacgat gccggctcca gtaccgagga
     6181 gctcgacggc gtgcgggaac gcggtggcca caactggcct accacaggcg atcgcgtcga
     6241 ccaggacacc agaagtgacc tgatcggtgg agtcataggg cagcacgacg accgccgccg
     6301 actgaatgag ggcaggtaac ttcgacgcgc cccagtagcg ggagtcgaag gtcaccgaat
     6361 cggctacgcc actgcggcgg gcttgttcca ttcgggcatc gcgatatgcc tcgccatcgc
     6421 cggagagcac cttcggatgc gtccgaccgg cgacgagata ccttgggcgg ccgggcaggt
     6481 ccttcaatga actcatcgac tcgatcaccc gctctacacc cttgccaggg cctaatagtc
     6541 cccacgtcag gagggtgggc cgactcggac gcttcagagc cgggccgctc gtgacggccg
     6601 cgccatgcgg gatcgtggtg accttatggc ggtccacgtc gaatccgaga cacaaccgat
     6661 gtctcgcagc ctcggacatg acaaccacat gatccgccat cgccgcgatc gcttccagaa
     6721 ccgaacgctg gtgtggcgtc ggatctttca gaattgtgtg ggcgacgacg atcgacggca
     6781 cagatagccc atcgatgacg tccaccacgt cgtctccgtc gacaccacca tagattccgt
     6841 actcatgctg aatcacggcg acgtcgttct ggttcagcag ctcggagctc gctccaacgg
     6901 acctcgccga accgctgacc agctccccaa tcacccgctc gctcaaggaa cgtgccccgt
     6961 cgtcagccac gcgaacgaca ctcacatcgg cgccattcgc gcacaaggcg tcagacaggg
     7021 cagcggtgaa cgtggccagt ccgcaatgcg tcggcgcata ggtactgagg atgccgacac
     7081 gagcagttcg ggaaaacctt tgcccctcaa caggatcgag aatcgacgta tagggggacg
     7141 gactggttgg aaag
//
LOCUS       DASYLX010000078         8394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1210852,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000078 DASYLX010000000
VERSION     DASYLX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210852"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1462
                     /locus_tag="VGP27_01915"
     CDS             155..1462
                     /locus_tag="VGP27_01915"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEV7850104.1"
                     /translation="MSCCHYQPTGGGLDTAFTVDASRITFGRGCLRELGERALGLGMT
                     RVALFSDPVVAGLEIFDTAQRSLRAAGLDVVAYTDVHVEPTDESFTRAAAFASHAKPD
                     GYVSVGGGSVIDTAKAANLYATHPADFLSYVNAPVGGGTPVPGSLEPHIACPTTSGTG
                     SEVTGIAIFDLLSMSAKTGIASPALRPTEALVDPDCTDSLPDQVVACSGLDVLSHALE
                     SFTARPFVRRPAPGKPSLRPMSQGANPWSDLGSREALRLLGQYLERAVSDASDREARE
                     QTMWAATLAGIAFGNAGVHIPHAMSYAVAGQVRGFHPDGYPGSEPLVPHGMSVILNAP
                     SAFRFTASTDPQRHIEAARHLGAEVGDAAPEDAGEVLAEELVRIMRAVGMPHGLSAVG
                     YAEADCATLADGAWPQQRLLQNAPIESDKSSLAQMFHSALRYW"
     gene            complement(1469..1918)
                     /locus_tag="VGP27_01920"
     CDS             complement(1469..1918)
                     /locus_tag="VGP27_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7850105.1"
                     /translation="MSEYRMVVVGTDGSDSSFRAVDKAAAIAAEADARLIVATAYLPT
                     EDHHSGEPDQLKGENYRTAGDAPVYGMLHEAGDRARAAGATDVDERAIAGAPIDVLVD
                     LVDEVDADLLVVGNVGVKSIAGRLLGSVPTAVRRRAKTEVLIVETTD"
     gene            2024..2308
                     /locus_tag="VGP27_01925"
     CDS             2024..2308
                     /locus_tag="VGP27_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850106.1"
                     /translation="MLAEFGRRADAAMTRGADAAHEAVHATALHARRILGAVSVALTR
                     VTQEASDLVWDYQDVAADLRRRDDGKTRPQKAELPQEQSRPALRVVGSDE"
     gene            complement(2318..4591)
                     /locus_tag="VGP27_01930"
     CDS             complement(2318..4591)
                     /locus_tag="VGP27_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850107.1"
                     /translation="MSPPPPPMITVCSGDVRRTFRAGRDIIVGRDVRADLRIPHPAIS
                     RAHMILRCVDGQWTAIDNDSLNGMFVGTERVRCLPVLDRTAINLGDPEGPRLTFELGP
                     APDEPPTVDTERPALQSITIGRAADNDICVSDMMASRHHATLVATPSGPQIHDAHSDN
                     GTFVNGERIKRKALQENDVVTIGNVDFVFVDGDLVRRTEPAATTGGLEVHDVGLTLGD
                     GDGTLLDRVSLTARPGALTAVIGPSGSGKSTLLKTMVGVWQPTSGTVRFDGRDLHAEY
                     ESLRSRIGMVPRLDIVHRTLTIAQALNIGVRLRMPADITKDERRQVVSRVLQELEMTE
                     HADTRVDNLSGGQRKRVSVAMELLTEPSLLVLDEPTTGLDPALDRQVMTMLRRLADAG
                     RVIVVVTPSLRFLDVCDQVLLLAPGGRTAYRGSPDGVAEAMGSEDWADIYEDIRADPD
                     GAQRRYFARHGSTADPVARPAAQASRVTRHSRSTSFWRQVSAIAQRQVWLLVADRRYL
                     VFLVLAPIVVGLLPLAVGGDAGFTKPDGGTAAPFEPRQIIVLLNFAAILMGLTVSVGD
                     LVGERTIYRHEQAAGLSPSAYLLAKLAVFGAVAVIQSALLVLVVTAPGIGKRAPATAA
                     VLGSPTLELFVDVAATAVVAAVLGLAISAVSSSSNQYIPLLAVACVAQLVLAGGFVPI
                     TDHPVLEAIAALTPARWGVAATASSVDLANLVPTVNDPLWRHSASVWLFDIAMLAVLA
                     LLYAGLVRWLLRRKATA"
     gene            complement(4649..5137)
                     /locus_tag="VGP27_01935"
     CDS             complement(4649..5137)
                     /locus_tag="VGP27_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7850108.1"
                     /translation="MDGYRTVVVGTDGSESSMRAVTRAGEVAAQENAKLIIASAHIET
                     VEKGGWSRAPSHDHVSDPRAADSLGGEGYILHGNAPVYGILREAHDKASAAGARHVEE
                     RAIDGAPVPALVKLVNEVNADLLVVGDVGLDSVAGRLLGSVPAEVARRARIDILIVHT
                     AD"
     gene            complement(5208..5480)
                     /locus_tag="VGP27_01940"
     CDS             complement(5208..5480)
                     /locus_tag="VGP27_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV7850109.1"
                     /translation="MSSQRVPGGVVHELPQDLRDALLGNSPALVAWHDITPLARNEFI
                     CWVEDAKQEKTRERRIRRTQEELEEGKRRPCCWPGCKHRERTGKPA"
     gene            5603..6673
                     /locus_tag="VGP27_01945"
     CDS             5603..6673
                     /locus_tag="VGP27_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEV7850110.1"
                     /translation="MTTLHDTGVRGFASDNYSGVHPEVLAAIAAANDGHQVSYGEDLY
                     TARLREVCARHFGAGVDTFPVFNGTGANVVGLLSMLPRWGAVICAKTAHIHADEGGAP
                     EKAAGIKLLPIAAADGKLTPDLIAEEAWGWGDEHRAQPLVVYITQSSELGTVYTPDEV
                     RAITGYAHERGMRVFMDGARLSNAAASLDVPLRAFTTDAGVDVVTFGGTKNGGLGAEA
                     IVVLDADACEGLVYLRKTQMQLASKMRFVSAQLLALLDDDLYLRAARHANAMAARLRS
                     QMEASIADGSVHGVEFTQATQANAVFATLPPGVADRLRESFRFYDWDTSRGEVRWVCS
                     FDTQPDDVDAFVAEAARLTSSA"
     gene            complement(6682..>8394)
                     /locus_tag="VGP27_01950"
     CDS             complement(6682..>8394)
                     /locus_tag="VGP27_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7850111.1"
                     /translation="KPLPRHRVGPLARGRNDWEATMFTIDEIATIPLFSTLEDKELRY
                     LAGEVEDIHVGPGEYVAHEGDGRALAVVVEGKAELTKLVNGVERVIGVRLPGELGGEV
                     PMMLGTPLPASMRAVGPSRVLKLSGEVFHTLVAMAPQVSAEISAAASERMGMLESATA
                     QPPETKMFVVGPRLDPGVHSCDNFLHRNQIPYERLDPDDPRAIARARGDGPMTGPYPV
                     VLLGDGTQMTAPSMRAVATVSGLTVSPGRPHYDVVIVGGGPAGLTAAVNGASEGLDTG
                     MIESFAPGGQAGTSTRIENYTGFPYGISGDELASRALQQAKRLGAEIVVTRRVESIDP
                     KDMTVTLDGGEVLRTRSVILAMGVEWRRLAIESVDRFVGSGVYYGAARSDASLAQGID
                     VYLVGAGNSAGQAAIFFSNYARSVTLLVRGEALAETMSTYLVEQIASKANIRVQTKSE
                     LVAAHGVERLESIEVIDRRTGTVSEREARTLFVLIGAQAATDWLPQEISRDRNGFVLT
                     GAEAMRTGQWKTDREAFTLETSAPGIFAVGDIRSGSVKRVAASVGEGGVAIALVHRYL
                     QLSS"
BASE COUNT         1378 a         2881 c         2718 g         1417 t
ORIGIN      
        1 gtcaacggcg cggcaatagg catggacgag ctttcgcagc tggaattcgt cgatcatctc
       61 ctgcagtacg gggtcggttg ccattgcgcc ttccggggtc atcagcgctt ccgctgcatc
      121 gttataggct cacctgtagg caaggggagg ctcgatgagc tgctgtcatt atcaacccac
      181 cggtggcgga ctggataccg cattcaccgt cgacgcatcc aggatcacct tcggcagggg
      241 ctgcctgcgg gaactgggtg agcgcgccct cgggctcggg atgaccaggg tggcgctgtt
      301 ctccgatccc gtcgtggccg gcctcgagat cttcgacacg gcgcagcggt ctttgcgggc
      361 agcgggcctc gacgtcgttg cctacaccga cgtccacgtc gagccgacgg atgaatcatt
      421 cacccgggca gcggcattcg cgtcgcatgc caagcccgat ggttatgtgt ccgtcggcgg
      481 cggctcggtg atcgacaccg ccaaggcggc caatctgtat gccacccacc ccgccgactt
      541 cctgtcctac gtcaatgcgc cggtaggtgg gggcacgccg gtgcccgggt ccctcgaacc
      601 gcacatcgcg tgccccacga cgtcgggcac cggcagcgag gtgaccggca tcgccatctt
      661 cgatctgctg tcgatgtcgg cgaagaccgg catcgcctca ccggcgctgc gtccgaccga
      721 agccctcgtc gaccccgact gcaccgacag cctgcccgat caggtcgtcg cctgctcggg
      781 actcgacgtg ctgtcccacg ccctggaatc gttcaccgcg cggcccttcg tccgccgccc
      841 cgcacccggc aagccgagcc ttcggccgat gagccagggc gccaacccgt ggagcgacct
      901 cggcagccgg gaggccctgc gactgctcgg tcagtacttg gaacgagccg tcagcgacgc
      961 gtccgaccgc gaagcgcgtg agcagaccat gtgggccgcc acactcgcgg gcatcgcatt
     1021 cggcaatgcg ggagtgcaca tcccgcatgc catgtcctac gcggtggccg ggcaggtgcg
     1081 cggcttccat ccggacggct acccaggatc cgaacccctt gtcccgcacg gtatgtcagt
     1141 gatactcaac gcaccttcgg ctttccggtt caccgcgtct accgatcccc agcgtcacat
     1201 cgaggccgcc cgtcacctcg gcgccgaggt gggcgatgcc gctcccgaag acgcgggtga
     1261 ggtcctcgcc gaggaactcg tacggatcat gcgagcagtg ggcatgcctc atggattgtc
     1321 cgccgtgggc tatgccgagg cggactgtgc cacgctcgcc gacggcgcgt ggccgcaaca
     1381 acgcctcctg cagaatgccc cgatcgagag cgacaagtcg tcgttggcgc agatgttcca
     1441 ctctgcactt cgctattggt gactgtgttc agtccgtggt ctcgacgatc aacacctcgg
     1501 tcttcgcccg acgacgtacg gccgttggca cagatccaag caggcgacct gcgattgatt
     1561 tgacccccac gtttccgacc accaacaggt cggcgtcgac ctcgtcgacc agatcgacga
     1621 gcacatcgat cggggcaccg gcgatggccc gctcatcgac gtctgtagcc cccgccgccc
     1681 tggcccggtc gccggcttcg tgcagcatcc cgtagaccgg cgcgtcgccc gcggtccggt
     1741 agttctcacc cttgagctga tcgggttcac cggagtggtg atcttcggtc ggcaggtatg
     1801 ccgtggccac gatcagcctg gcgtcggcct cggctgcgat tgctgcagct ttgtcgacgg
     1861 cacgaaacga cgagtccgac ccgtcggtcc cgacgaccac catcctgtat tcgctcatca
     1921 gccctccgct cgggtgtcat ttacccgcac cggcccgatt ccatacgttc agcgggggat
     1981 gatggtcagt agccacgtcg ctaagagccg tcgaggagga ccgatgcttg ccgagttcgg
     2041 ccgcagggca gatgccgcca tgacccgagg agccgacgcc gcccatgagg ccgttcacgc
     2101 cacggctctg cacgcgcgaa gaatcctcgg ggcggtttcc gtcgcgctca cccgcgtgac
     2161 gcaagaggct tccgacctgg tgtgggacta ccaggacgtc gcagccgact tgcgccgacg
     2221 cgacgacgga aagacgcgtc cgcaaaaggc cgagcttccc caggagcaga gtcggcccgc
     2281 cttgcgggta gtcggttccg acgagtaaac gacgccgcta cgccgtggcc tttcgccgca
     2341 gtagccaccg caccaacccg gcgtacaaca gggccagcac tgccagcatc gcgatgtcga
     2401 acagccacac cgatgcgctg tgccgccaca gcggatcgtt caccgtcgga acgagattag
     2461 ccagatcgac cgacgacgcc gttgcggcga ccccccagcg cgccggagtg agggcggcga
     2521 tcgcttcgag cactggatgg tccgtgatcg gaacgaaacc gcctgcgagg acaagctgag
     2581 ccacacaagc gaccgccagc agtggaatgt actgattgct gctgctcgag acagccgaga
     2641 tcgcgaggcc gaggaccgcc gcgaccacag cggtcgcggc cacatcgacg aacaactcca
     2701 gcgtcggact gccgagcacg gcggcggtcg ccggcgcgcg cttgccgatg ccgggtgcag
     2761 tgacgaccag cacgagaagc gctgactgaa tcacggcgac cgcgccgaac acggcgagct
     2821 tggccaacag gtacgccgac ggcgaaagcc ccgccgcctg ctcgtgccgg tagatcgtcc
     2881 gctcgccgac caggtcaccc accgataccg tcaaccccat caggattgcg gcgaagttga
     2941 gcagcacgat gatctgccgg ggctcgaacg gtgcggctgt gccgccgtcc ggtttggtga
     3001 agcctgcatc gccgccgacc gccaacggca gcaggccgac gacgatcggc gccagtacga
     3061 ggaagacgag atagcggcgg tcagcgacaa ggagccacac ctggcgctgc gcgatcgccg
     3121 agacctggcg ccagaaactt gtgctcctgc tatgtcgcgt gacccgggac gcctgcgccg
     3181 ctggtcgcgc cacggggtct gccgtcgacc cgtgccgcgc gaagtaacgc cgttgcgcgc
     3241 catcgggatc cgcgcggatg tcttcgtaga tgtcggccca atcctccgag cccatcgcct
     3301 cggcgacccc atcgggtgag ccgcggtacg cggtcctgcc accgggggcc aacagcaaca
     3361 cctgatcgca tacgtccagg aatcgcagcg acggcgtcac caccacgatc acgcggccgg
     3421 catcagcgag gcgacgcagc atcgtcatca cctgccggtc cagcgcgggg tccaggcccg
     3481 tcgtgggttc gtcgagcacc agcagcgacg gttcggtcaa cagttccatc gcgaccgaca
     3541 cgcgcttgcg ctgtccaccc gagaggttgt ccacccgcgt atcagcgtgc tcggtcattt
     3601 ccagttcctg aagcactcgc gaaacaacct gtcggcgttc gtccttggtg atatcggctg
     3661 gcattcgcag acgcaccccg atgttcagcg cctgggcaat ggtgagcgtc cggtgaacga
     3721 tgtcgagccg tggcaccatg ccgatgcggc tgcgcagcga ctcgtactcg gcatggaggt
     3781 cgcgtccgtc gaacctcacc gtgccactcg tcggctgcca aaccccgacc atcgttttca
     3841 acaacgtgga cttacccgag cccgacgggc cgatcacggc cgtcagcgca ccgggccgag
     3901 cagtgaggga aacccgatcc agcagcgtcc cgtcgccgtc gccgagcgtc aaccccacgt
     3961 cgtgtacctc caatccgccg gtcgtggcgg cgggttccgt gcggcggacg agatcgccgt
     4021 cgacgaatac gaagtcgaca ttgccgatcg tgacaacgtc gttctcctgc aacgccttgc
     4081 gcttgattcg ctcgccgttg acgaacgtgc cgttatcgct gtgcgcgtcg tggatttgcg
     4141 gccccgacgg cgtcgccacc aacgtggcgt gatgccgcga cgccatcatg tcggaaacac
     4201 agatgtcgtt gtcggcggca cgcccgatcg tgattgattg cagcgcgggc cgctcggtat
     4261 cgacggtcgg cggttcgtcg ggcgcaggac cgagttcgaa cgtcagccgc gggccttcgg
     4321 ggtcgccgag gttgatggcg gtgcgatcga gcacgggcag acaccgcacc cgctccgtgc
     4381 cgacgaacat gccgttgagg ctgtcgttgt cgatcgcggt ccactgtccg tcgacgcatc
     4441 gcaggatcat gtgcgccctg gagatcgccg ggtgcgggat acggagatcg gcgcggacgt
     4501 cgcgcccgac gatgatgtcg cggccggccc ggaaggtgcg tcggacgtcg ccggagcaga
     4561 cggtgatcat gggcggtggt ggcgggctca tccgccgcga acccccagcc ggtcaccggc
     4621 gccgttcacc ggctcacgat agaccgccct agtcggcggt gtggacgatc aagatgtcga
     4681 ttcgcgccct gcgggcgact tcggcgggca ccgagcccag cagccggcct gcgacggaat
     4741 cgaggcccac gtctccgacg acgagcagat ccgcgttcac ctcattgacc agtttcacca
     4801 gtgccgggac cggcgcaccg tctatcgccc gctcctcgac gtgccgagcg cctgcagccg
     4861 aagccttgtc gtgcgcctcc cgaaggatgc cgtagacggg agcgttgccg tgcagtatgt
     4921 agccttcgcc gccgagggag tccgcggcgc gggggtcgga cacatgatcg tgcgacggcg
     4981 ctcgtgacca accacctttc tcgacggtct cgatgtgcgc gctggcgatg atcagcttcg
     5041 cgttctcctg tgcggcgacc tctccggcgc gcgttaccgc gcgcattgac gactccgagc
     5101 cgtcggtccc gaccaccaca gtccgatacc cgtccacgtc gcctcctcag ctcagtcgcg
     5161 actagtgacg gtatctccgg cccaccggct gtgggcccga ttggcaacta tgcgggcttg
     5221 ccggtgcgct cgcgatgctt gcagcccggc cagcagcatg gccgccgctt gccttcctcc
     5281 agctcctctt gggtccgtcg gatgcggcgc tcgcgcgtct tctcctgctt ggcgtcctcg
     5341 acccagcaga tgaactcgtt gcgcgccaac ggcgtgatgt cgtgccacgc gacgagggcg
     5401 ggggagttgc cgagcagtgc gtctcgcagg tcttggggca attcatggac cactccgccg
     5461 gggactcgct ggctgctcac gaagtcaggc tagccgcagt aggttcgtcg atgccgcgac
     5521 ctaagtcacg agttgcgagc agaaatgggc ggtgatctcg ccctcacaga acccaccggc
     5581 agtggtccta gatttagcaa ccgtgactac tttgcacgac acaggagttc gcggcttcgc
     5641 ctccgacaac tactcgggtg tgcacccaga ggtgctggcc gcgatcgccg ccgccaacga
     5701 cggacaccag gtctcttacg gcgaggacct gtacaccgcc cgcctgcggg aggtgtgtgc
     5761 gcgccacttc ggcgcgggcg tcgatacctt tccggtgttc aacggcaccg gtgccaatgt
     5821 cgtcgggctg ctgtcgatgc tgccgcggtg gggagcggtc atctgcgcga agacggcgca
     5881 catccacgcc gacgagggtg gggcgccgga gaaggccgcc gggatcaagc tgctgccgat
     5941 cgccgcggcc gacggcaagc tgacgcccga cctgatcgcc gaggaagcct ggggctgggg
     6001 cgacgagcat cgggcgcaac cgctcgttgt ctacatcacg cagtcgagcg aactcgggac
     6061 ggtgtacacg cccgatgagg tgcgggcgat caccggctat gcgcacgaac gcggaatgcg
     6121 cgtcttcatg gacggcgcgc gtctgtcgaa tgccgccgcg agcctcgacg tgccgctgcg
     6181 cgcgttcacc accgacgccg gcgtcgacgt cgtgaccttc ggcggcacca agaacggcgg
     6241 gctcggcgca gaggcgatcg tggtgctcga cgccgacgcc tgcgagggcc tggtctatct
     6301 gcgcaaaacc cagatgcagc tcgcgtcgaa gatgcgattc gtctcggcgc agctgctggc
     6361 actgctcgac gacgacctct acctgcgcgc cgcccggcac gcgaacgcga tggccgcgcg
     6421 gctgcgttcg cagatggagg cgagtatcgc cgacggcagc gtccacggcg tcgagttcac
     6481 ccaggcgacg caggcgaacg cggtgttcgc cacgttgccg ccgggcgtcg ccgaccggct
     6541 gcgcgagagc ttccgcttct atgactggga cacatcgagg ggtgaggtgc gctgggtgtg
     6601 cagtttcgac acgcaacctg acgatgtcga tgcgttcgtc gctgaggccg cccgcttgac
     6661 gtcgtctgcg tgaccaaacg cttacgagga gagctggagg tagcggtgca ccagggcgat
     6721 cgccacaccg ccctcaccga cagacgctgc tactcgcttc accgagccgg agcggatgtc
     6781 gccgacggcg aagattccgg gtgcgcttgt ttcgagagtg aacgcctcgc gatctgtctt
     6841 ccactgtcct gtcctcatcg cctcggctcc tgtcaggacg aagccgttcc tgtcgcgcga
     6901 gatctcctga ggaagccagt ccgtcgcggc ctgggctccg atgagaacga agagcgttcg
     6961 cgcctcgcgc tcagataccg tgcctgtcct gcggtcgatg acctcgatgg actcgagccg
     7021 ttcgactccg tgcgccgcga cgagttcgga tttcgtttgc acccggatat ttgccttcga
     7081 tgcgatctgc tcgaccaggt aggtcgacat ggtttcggcc agcgcttcgc cccgaacgag
     7141 caacgtcacc gaccgcgcat agttggagaa gaagatggcg gcctgacccg ccgagttgcc
     7201 ggctccgacc aggtacacat cgattccctg cgccaggctg gcgtcgctgc gcgccgcgcc
     7261 gtagtagaca ccggagccga cgaatcggtc gaccgactcg atcgccagac gtcgccactc
     7321 cacgcccatc gcgaggatga ccgacctggt ccgcaagacc tctccaccgt ccagcgtcac
     7381 ggtcatgtct ttcgggtcga tgctttcgac ccgtcgggtg acgacgatct ctgcgcccaa
     7441 ccgcttcgcc tgttgcagcg cccggctcgc gagttcgtcg ccggagatgc cgtatgggaa
     7501 tccggtgtag ttctcgatcc gcgtcgaggt accggcctga ccgccgggcg cgaaggactc
     7561 gatcatcccg gtgtccaagc cttcggaggc gccgttgacg gcagctgtca gacctgccgg
     7621 accgccgccc acgatcacga cgtcatagtg tggccggcct ggtgagaccg tgaggccgga
     7681 gaccgtggcg acggcgcgca tcgacggtgc ggtcatctgc gttccgtcgc cgagcaacac
     7741 caccggatag gggcccgtca tcgggccgtc tcctcgcgcg cgtgcgatcg cgcgaggatc
     7801 gtccggatcc aggcgctcgt aggggatctg attgcggtga aggaagttgt cgcagctgtg
     7861 cacccccggg tcgaggcgcg gaccgacaac gaacatcttg gtctcgggcg gttgcgcggt
     7921 ggcgctctcg agcatcccca tgcgctccga cgctgctgcg ctgatctcgg cggatacctg
     7981 aggcgccatg gctacgagcg tgtggaacac ctcgccgctc agcttcagca cgcgcgacgg
     8041 accgacggca cgcatgctgg cgggcaaggg cgtgccgagc atcatcggaa cttcgccgcc
     8101 cagctctccg ggaagccgga cgccgatcac ccgctcgaca ccgttcacca gcttggtcag
     8161 ctccgctttg ccctcgacga cgaccgccag ggcacggcca tccccctcgt gagccacata
     8221 ttcccctggc cccacgtgga tgtcctcgac ttcgccggca aggtacctca gctctttgtc
     8281 ctcaagcgtc gagaagagcg gaatcgtcgc gatctcgtcg atggtgaaca tggttgcctc
     8341 ccaatcattt cgcccgcggg ccaatggtcc gacacggtgt cggggaagcg gctt
//
LOCUS       DASYLX010000079         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_114101,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000079 DASYLX010000000
VERSION     DASYLX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114101"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGP27_01955"
     CDS             <1..877
                     /locus_tag="VGP27_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV7850112.1"
                     /translation="RRGRSSFAPDSGHSLEGLVNAHALTRTVRDSAALLDVIAGSAPG
                     DPYTAAPPATTYLDAITKDPPPQRILLATASPFPGPPTDPAVMAAVENTAQLLGEIGH
                     FVEPGFPAIDSDAVADAIAILHTVSNAALHALATNHLGHEPAEDEFEPSTWVMVREGF
                     TTTGAAYAGAIDAVHAQTRRFAAGMAGHDVLLVPTLLTPPPPYALLDQPRGTTRAFFD
                     VEFATTGWTSLANVTGWAAISLPLGSTRDGLPIGVQLVAPDETVLLQLAAQIESAAPW
                     AGRRPPKWVGADSSR"
     gene            complement(986..1282)
                     /locus_tag="VGP27_01960"
     CDS             complement(986..1282)
                     /locus_tag="VGP27_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850113.1"
                     /translation="MTTQRKTVSRLFDIRNIIGALLAIYGVLLTIAGLVPGLVASHDD
                     AAAAGNKMDLYVGTDANWWVGLALLAVAAVFLAWAALRPLVVEVESDSAAADEA"
     gene            complement(1315..3033)
                     /locus_tag="VGP27_01965"
     CDS             complement(1315..3033)
                     /locus_tag="VGP27_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEV7850114.1"
                     /translation="MGTTTTTLAAESVLRLHVGALDYLLIAIYFVFVLGIGYLARSQI
                     STSLDFFLSGRRLPAWVTGLAFVSANLGAVEIMGMSANGAQIGLSTMHYYWIGAIPAM
                     VFLGIVMMPFYYGSKVRSVPEFMRRRFGPGAHLVNAISFGVAQVLIAGVNLFLLATVI
                     NVLLGWNRWLSLIVAAVIVLTYTALGGLSAAIYNEVLQFFVILAALVPLTIFGLIKVG
                     GWRGLHDKVIDAVKDDGTVTASVHEQLTSWPGQALSGFDSPVWSVIGIVFGLGFVLSF
                     GYWTTNFVEVQRAMASDSISASRRAPIIASFPKMFIPFVVVVPGMIAAAAIGDMIHLK
                     STGSGDITYNDAMLLMIRDILPNGLIGVAVAGLIASFMAGMAANISAFNTVFSYDIWQ
                     QYIVKDRSDRYYIGVGRIATVAATVLAVFTALIAAGYSNLMDYLQTLFGFFNAPLFAT
                     FILGMFWKRMTATAGWAGLVAGTLSAIAVFVLSEVGVINLPGQGMPFLAATAAFIVDI
                     VVSVVVSLVTTPKPVAELAGFVYSETPVQIFADPESDGKAWYMRPVPLGGVVLALAIV
                     LNIIFH"
BASE COUNT          588 a         1014 c          965 g          485 t
ORIGIN      
        1 gcgccgtggc cggagctcgt tcgcgccgga ctcgggccac tcactggagg gtttggtcaa
       61 cgcacacgcg ctcacccgta cagtgcggga cagcgccgcc ctgctcgatg tcatcgcggg
      121 cagcgccccc ggcgacccct ataccgccgc tccgccggcc accacgtacc tggatgccat
      181 caccaaagac ccaccgccac aacgtattct gctggcgact gcttcgcctt ttccgggtcc
      241 gccaaccgat cccgctgtca tggccgccgt cgagaacacc gcacagctac tcggcgaaat
      301 cggtcacttc gtcgaaccgg gctttcccgc gatcgattcc gatgccgtcg ccgacgcgat
      361 cgcaattctg cacaccgtca gcaacgccgc gcttcacgcg ttggcgacga atcatctcgg
      421 ccatgaaccc gcggaagacg aattcgagcc gagcacgtgg gtgatggttc gcgagggctt
      481 caccactacg ggcgccgcat atgccggtgc catcgacgcg gtgcatgccc agacgcgacg
      541 cttcgcggcc ggcatggcgg gccatgacgt gctgctcgtg ccgacattgc tgacgccccc
      601 gccgccctac gcgctgctgg accaaccgcg cggcaccacc cgtgcgttct tcgacgtcga
      661 gttcgccacc acggggtgga cctcgctggc gaacgtcact gggtgggccg cgatctcact
      721 tccgttgggc agcactcgtg acggactgcc gatcggcgtg cagctcgtcg caccggacga
      781 aacggtcctt ctgcaactcg ctgcccaaat cgaaagtgcg gccccgtggg ccgggcggcg
      841 gccgccgaag tgggtcggtg cagactcgtc tcggtagctc gcctctcccg cgagcgcgcg
      901 tgtctgcaca tcgacacgcc gtaaagcgtc ggcactttgc gcacggtcac gcggcgacga
      961 gcgggcgctg aaagccaagc agtcctcacg cctcgtccgc ggccgcgctg tccgattcga
     1021 cttcgacgac aaggggccgt agcgccgccc atgccaggaa caccgccgcc acggcgagca
     1081 gagccaggcc gacccaccaa ttggcgtcgg tgccgacata taggtccatc ttgttgcctg
     1141 ctgcggccgc gtcgtcgtgt gaggcgacca gcccgggcac cagcccggcg atcgtgagca
     1201 ggacgccgta gatcgcgagc agtgcaccaa tgatgttgcg aatgtcgaac agtcgggaga
     1261 ctgtcttcct ctgggtcgtc atgttcactc ctgttgcgat tcaacgaaaa tgggtcagtg
     1321 gaagatgatg ttgagcacga tggccaacgc cagcacgacg ccgccgagcg gaacgggcct
     1381 catgtaccag gccttgccgt cggattcggg gtcggcgaag atctgcaccg gagtctccga
     1441 gtagacgaag ccggccagct cggcgaccgg cttgggcgtg gtgaccaggc tgacgacgac
     1501 gctgaccacg atgtcgacga tgaatgcggc ggttgcagcc aggaacggca tcccttgacc
     1561 gggcaggttg atgacgccga cttcggaaag cacgaaaacc gcgatcgcag agagtgttcc
     1621 ggcgacgagc ccggcccagc ccgcggtggc cgtcatgcgc ttccagaaca tgccgaggat
     1681 gaacgtcgcg aacagcggcg cgttgaagaa gccgaacagc gtctgcaggt agtccatcaa
     1741 gttcgagtag cctgccgcga tgagcgccgt gaacaccgcg agcaccgttg cggccaccgt
     1801 cgcgatccgc ccgaccccga tgtaatagcg gtcagaacgg tccttgacga tgtactgctg
     1861 ccagatgtcg tagctgaaga cggtgttgaa ggcagagatg ttggccgcca tacccgccat
     1921 gaacgatgcg atcagccccg ccaccgcgac gccgatgagt ccgttcggca ggatgtcacg
     1981 aatcatcagc agcattgcat cgttgtacgt gatgtcgccg gagccagtcg atttcaggtg
     2041 gatcatgtcg ccgatcgcgg cggcggcgat catcccgggc acgacgacca cgaagggaat
     2101 gaacatcttc gggaacgacg cgatgatcgg tgcccgacga ctggccgaaa tcgagtcgga
     2161 cgccatggcg cgctgcacct cgacgaagtt cgtcgtccag tagccgaacg acagcacgaa
     2221 tccgaggccg aagacgatgc cgatcaccga ccacaccggg ctgtcgaacc cgctcagcgc
     2281 ctggcccggc cagctggtca actgctcgtg caccgaggcc gtcaccgtcc cgtcgtcctt
     2341 gacggcgtcg atcaccttgt cgtgcaaccc acgccacccg ccaaccttga tcaggccgaa
     2401 gatggtgagg ggcaccagcg cggccaggat cacgaagaac tgcaacacct cgttgtagat
     2461 tgcggccgac agcccgccca gcgcggtgta ggtcagcacg atgaccgccg ccacgatcaa
     2521 cgacagccac cggttccagc caaggagcac gttgatcacg gtggcgagca ggaacaggtt
     2581 gacgccggcg atcagcacct gcgcgacccc gaagctgatg gcattgacca gatgcgcgcc
     2641 cggcccgaac cgcctgcgca tgaactccgg gacgctgcgc accttggagc cgtaatagaa
     2701 gggcatcatc acgatgccga ggaacaccat cgcgggtatg gcgccgatcc agtagtagtg
     2761 catcgtcgac agcccgatct gggccccgtt ggccgacatt cccatgatct cgacggcgcc
     2821 gaggttggcg gacacgaatg ccagcccggt cacccaggcg ggtaggcggc ggccggagag
     2881 gaagaagtcg aggctggtgg agatctggct gcgagccaga taaccgatgc cgagaacgaa
     2941 gacgaagtaa atcgcgatca gcaggtaatc cagtgcgccg acgtgcagac ggagcacgct
     3001 ttcggccgcc agcgtggtgg tcgttgtccc cacctccttt tcgttgagat tc
//
LOCUS       DASYLX010000080         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3405109,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000080 DASYLX010000000
VERSION     DASYLX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405109"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGP27_01970"
     CDS             <1..666
                     /locus_tag="VGP27_01970"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEV7850115.1"
                     /translation="ADAIPHAYPRGKKDPDARANVAAEAAPEFMPLVRRLSRLLPPDV
                     RVTDIVRAPAGFDARFSALRRHYEYRLSTAPYGVDPQDARYVTAWPRPLDVEAMTVAS
                     RELIGLHDFAAFCRYREGATTIRDLQRLDWSMSGDLVTAHVTADAFCWSMVRSLVGAL
                     LAVGEGRREPAWCAGLLDASRRSSDFAAAPPRGLTLVGVDYPPDDQLEARIVVTRDVR
                     RFD"
     gene            complement(663..1346)
                     /locus_tag="VGP27_01975"
     CDS             complement(663..1346)
                     /locus_tag="VGP27_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7850116.1"
                     /translation="MGQRIRRALLAAVAASLVAAGLLVGPSILAGNKAIPTASAACPQ
                     VQVVFARGRLESPGVGVLGNAFVSALRKKVNKNIDVYAVRYPADNEVDVGANDMSAHI
                     QSMANACPDTRLVLGGYSLGAAVTDVVLALPFSFFGFDNPLPADMEQKIAAVAVFGNG
                     SAWAGPITNFSPTYSERTIELCHGADPICNPADPNTWEQNWVDHAAKAYIDAGMVNQA
                     ADFVAGRLN"
     gene            complement(1427..>2057)
                     /locus_tag="VGP27_01980"
     CDS             complement(1427..>2057)
                     /locus_tag="VGP27_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7850117.1"
                     /translation="VETTAALLVGAAAAITPSIAFAPVASADCPDIEVVFARGTDEGA
                     GLGRIGGAFVDSLRGKVGGRSVGAYAVNYPASYDFLAAASGANDASAHIQYMMGACPN
                     TKLVLGGYSQGAAVMDVIAAVPIPAIGFDNPLPPNAPDFVPAIAVFGNPSAKLGIPLT
                     MSPVWGGRAIDLCNPDDPICQTDGESVPAHRAYNGGPVDTAADFVAGRV"
BASE COUNT          345 a          677 c          689 g          346 t
ORIGIN      
        1 gccgacgcga tcccgcacgc ctacccgcgc gggaagaagg atcccgacgc ccgagccaat
       61 gtcgccgccg aagcggctcc ggagttcatg ccgttggtcc gacggctgtc gcggctactg
      121 ccgcccgatg tcagggtgac cgatatcgtc cgggcaccag cgggtttcga cgcacggttc
      181 tcggcgctgc gacgtcatta cgagtaccgg ttgtccaccg ccccctacgg cgtcgatccg
      241 caggatgccc ggtacgtgac cgcgtggccg cgtccgctgg acgtcgaggc gatgacggtc
      301 gcttcgcgcg aactcatcgg gctgcacgac ttcgcggctt tctgccgata ccgcgaggga
      361 gccaccacga tccgggacct gcaacggctg gactggtcga tgtccggcga tctcgtcacc
      421 gcccacgtca ccgccgacgc gttctgctgg tcgatggtgc gctcgctcgt cggcgcccta
      481 ctcgcagtgg gtgagggccg tcgcgaacca gcttggtgtg cgggcttgtt ggacgcgtca
      541 cgtcgctcca gcgatttcgc agccgcaccg ccgcgaggcc tgacgctcgt cggcgtcgac
      601 tatccgcccg acgaccaact cgaggcgcgc atcgtggtga cccgcgacgt gcgccgcttc
      661 gactagttca accggcctgc gacgaagtcg gcggcctggt tcaccatgcc ggcgtctata
      721 tatgccttcg ccgcatggtc aacccagttc tgctcccacg tattgggatc cgccgggttg
      781 cagatcggat cggcaccgtg gcacaactcg atggttcgct cggagtaggt cgggctgaaa
      841 ttcgtgatcg gccccgccca ggccgagccg ttgccgaaca cagcgacggc agcgatcttc
      901 tgctccatat cggccggcaa gggattgtcg aagccgaaga acgagaacgg aagcgcgagg
      961 accacgtcgg tgaccgcggc acccagtgag taaccgccga gtaccaggcg ggtgtcgggg
     1021 caggcgttgg ccatgctctg gatgtgcgcg ctcatatcgt tggcgccgac gtcgacctcg
     1081 ttgtcggcgg gatagcgcac cgcatacaca tcgatgttct tgttcacctt tttgcgcagc
     1141 gcgctgacga acgcatttcc gagcaccccg acgccgggcg actccagacg gccgcgggca
     1201 aagaccacct ggacctgcgg acaggcggcc gacgccgtcg ggatggcctt gttgccagcc
     1261 aggatggatg gtcctaccag cagaccagcc gccacgagac tcgcggcgac tgcggccagt
     1321 aaggcgcgcc gaatccgttg acccatgctg ttgcgttcga cagggatgac caccacgccg
     1381 atgttagccg acgagttcac gcgcccgttc taacgcacgg tgtgagctac acgcggcctg
     1441 cgacgaagtc ggcggcggtg tcgaccggtc cgccgttgta ggcacggtgc gcgggaacgc
     1501 tttcgccgtc ggtctgacag atcggatcgt cggggttgca cagatcgatg gcccgtccac
     1561 cccacaccgg gctcatcgtc aacgggatgc cgagcttggc cgatggatta ccgaaaacgg
     1621 cgatcgccgg tacgaagtcg ggggcgttgg gtggcagagg gttatcgaag ccgatcgcgg
     1681 gaatgggcac cgcggcgatg acgtccatca ccgcagcgcc ttgcgagtag ccgccgagca
     1741 ccaacttggt gttcgggcag gcacccatca tgtactggat atgcgcgctg gcgtcgttgg
     1801 cgccgctggc ggcggcgagg aagtcgtagc ttgccggata gttcacggcg taggcgccga
     1861 ccgatcggcc gccgaccttg ccgcgcaacg aatcaacgaa cgcaccaccg atgcgcccga
     1921 gaccggcgcc ttcgtcagtg ccgcgggcga agaccacttc gatgtcggga cagtcggcag
     1981 aagccaccgg agcgaacgcg atcgacggcg tgatggccgc ggcggctccg acgagtaggg
     2041 cagctgttgt ctcaact
//
LOCUS       DASYLX010000081         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2746571,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000081 DASYLX010000000
VERSION     DASYLX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746571"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..115
                     /locus_tag="VGP27_01985"
     tRNA            44..115
                     /locus_tag="VGP27_01985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Val,seq:cac)
     gene            complement(246..1619)
                     /locus_tag="VGP27_01990"
     CDS             complement(246..1619)
                     /locus_tag="VGP27_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7850118.1"
                     /translation="MSSSRGGRRKRKGVDGSVYPRGKKWAYVVDLGPDPLTGERRRNS
                     RSGFPSEDEAWQALVDANQRLRSNTYVKNTPRTVRQFFDEWLAAMKMSLKPTTFNNYS
                     RYAEYYVLPVIGDRKLQELTTETITRLYAHLLENGRRRGNSNQLMYEEWKRSLAAGRE
                     ATPRELAEIGKVTYSAGVRAAQRYRAGRIPTDYSTGLDPRSVHSVHIMLNAALHDAVE
                     AKYIAENPVTKAGRIRQERRSHRVWTPDELRRFLAAAETDRFFPMWLLFATTGTRRSE
                     AAGARREHLDLTARTITLWETRVVAGGQAQKSDGKTARSRRQLALDRRTAAALVDHLT
                     VLDQERHDFGPDHQDHGLLFCWEDGRPLHPDTITEQFNRIVDRARMPPITLHDVRHTY
                     ATMSLRAGVNPKIVSARLGHATVAFTLDTYTEDVPELHHDAAEAVSNLFLADSEEGDA
                     DPPLATG"
     gene            complement(1616..>1967)
                     /locus_tag="VGP27_01995"
     CDS             complement(1616..>1967)
                     /locus_tag="VGP27_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850119.1"
                     /translation="AVEALHDLAAESKPRGRQRPGEQQGTAKEPTAWNLVKRLLPEQA
                     QHQVAPSMSDFDGLPVLISVRTAARILGISRASAYRYIDRGLLPVKRLGGRVYIVRDR
                     LRDMVESSEGVSTG"
BASE COUNT          259 a          594 c          722 g          392 t
ORIGIN      
        1 gaagagcccg atcctggggt gatgtagtgt tctccacggc agcgggcgat tagctcaggg
       61 ggagagcgct tcgttcacac cgaagaggtc actggttcga tcccagtatc gcccacgcag
      121 ccggaaggcc cctgaccagc aagttcaggg gccttcggtc ttttctgggg tcgatgcgcg
      181 ggcccgttcc gggcccgcgg tagccgaaac ggtagccaaa cggcttcggc ggcgtccgtc
      241 gtcgctcagc cggtagccaa cggcgggtcc gcgtcgcctt cctcactgtc ggcgaggaac
      301 aggttgctga cggcctcggc ggcgtcgtgg tggagctcgg ggacgtcctc ggtgtaggtg
      361 tcgagggtga acgcgacagt ggcgtgtccg aggcgggcgc tgacgatctt ggggttgacg
      421 cctgcacgca gcgacatcgt cgcgtaggtg tgccggacgt cgtgaagtgt gatcggtggc
      481 attcgtgcgc ggtcgacgat gcggttgaac tgctcggtga tcgtgtcggg gtggagaggg
      541 cgtccgtcct cccagcagaa cagcagcccg tggtcctgat gatcgggccc gaagtcgtgg
      601 cgctcttgat cgaggaccgt caggtggtcg accagtgcgg ccgccgtccg ccggtcgagg
      661 gcgagctggc ggcgggatcg tgctgtcttg ccgtccgact tctgagcctg cccgccggcc
      721 acgacccggg tctcccagag ggtgatcgtt cgggcggtga ggtcgaggtg ttcgcgtcgg
      781 gcgccggcgg cttcggatcg tcgagtacct gtggtggcga acagcagcca catcgggaag
      841 aagcgatcgg tctcggccgc ggcgaggaac cgccggagct cgtccggggt ccagacccgg
      901 tggcttcgcc gttcctgccg tatccgcccg gccttggtga cggggttctc ggcgatgtac
      961 ttggcctcga cggcgtcgtg cagggccgcg ttgagcatga tgtgcacgct gtggacgctc
     1021 cgtgggtcga ggcccgtgct gtagtcggtc gggatgcgtc cggctcggta gcgctgggct
     1081 gcgcgcaccc cggctgagta ggtcaccttg ccgatctcgg cgagttcgcg gggtgtggcc
     1141 tctcgtccgg cggccagcga ccgcttccac tcctcgtaca tgagttggtt cgagttgccg
     1201 cgccggcggc cgttctcgag gagatgggcg tagaggcgag tgatggtctc ggtggtcagc
     1261 tcttgaagct tgcggtcgcc gatgacgggc aggacgtagt actcggcgta ccggctgtag
     1321 ttgttgaagg tggtcggctt gagcgacatc ttcatcgcgg cgagccactc gtcgaagaac
     1381 tgccgcacgg tgcggggtgt gttcttgacg taggtgttcg accgaagcct ctggttcgcg
     1441 tcgaccaagg cttgccaggc ctcgtcctcg gacgggaacc cgctgcggga gttgcggcgg
     1501 cgctccccgg tgaggggatc gggtccgagg tcgacgacgt atgcccattt cttgcctcgg
     1561 ggatagacag acccatcgac tcccttgcgc ttacgtctgc ctccgcggct gctgctcatc
     1621 cggtgctgac tccctcgctg ctctcgacca tgtcgcggag ccggtcgcgc acgatgtaga
     1681 cccggccgcc cagccgcttg accggaagca ggccgcggtc gatgtagcgg taagcagacg
     1741 ctcggctgat gccgaggatc cgggctgcgg ttcgtacgct gatcaggact gggagtccgt
     1801 cgaagtcact catggatggc gccacctgat gttgggcctg ctctggcagc aggcgtttca
     1861 ccaggttcca ggcggtgggt tccttcgccg ttccctgctg ttccccgggc ctctggcggc
     1921 cgcgtggctt cgactccgct gccagatcgt gcagtgcttc gactgcg
//
LOCUS       DASYLX010000082         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2527171,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000082 DASYLX010000000
VERSION     DASYLX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527171"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..640)
                     /locus_tag="VGP27_02000"
     CDS             complement(74..640)
                     /locus_tag="VGP27_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEV7850120.1"
                     /translation="MRWPVKAVAIAAVTAVMTVAGCAQTVSGTAQRAHPSVPDPQRSY
                     GYLDNRCGLLEDSSIQQILGATNVVRPYSGAVCQYVLERGPGPDAGAGAPPNTTIDVV
                     FSWFETGSLDRERTFADQRGAKVTGKVVERHEAFLARRDTNGAGCSATAAAGPGVLSW
                     WVQTRGRTDSDPCADAEKLLTATLSSDL"
     gene            759..1145
                     /locus_tag="VGP27_02005"
     CDS             759..1145
                     /locus_tag="VGP27_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEV7850121.1"
                     /translation="MTAGGWEALSRSMVAFAVAVPVALTVSVPAAQAKNGDTHITSIG
                     GNQTIDCNESTLIVLGANNTINALGTCWAVTVQGSGNTIVADNVINDITVYGFDQAVF
                     YKNGEPAVWDRGRELGMTNRIDRIPA"
     gene            1189..1590
                     /locus_tag="VGP27_02010"
     CDS             1189..1590
                     /locus_tag="VGP27_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEV7850122.1"
                     /translation="MRTRVLIGVTAAAAAVGLAGCGAESSDSNTPTATLGSGGGQVEI
                     GNTINYSSVGTTTELDCADGKSLNIGGNNNKLTIKGTCANANIGGADNTVTMDRVDKG
                     LSVVGFNNTVTYKQGDPKINDTGSGNKISKG"
     gene            1639..2244
                     /locus_tag="VGP27_02015"
     CDS             1639..2244
                     /locus_tag="VGP27_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850123.1"
                     /translation="MYIAVDMKSRKYDMGARQRAKSATRDAIIKAAIDTFMAERSFAL
                     TLPTVAERADVTVKTVLRHFGSRDGLIEAAWSQAYDEVMAERIPPPGDLEGALKVLIA
                     HYEARGEVVLAMLTEENDPRAARMNSAGRLGHRHWVEEVFADRLPAGTDERTRFIDVL
                     VVATDVYTWKLLRLDRGLSVDDVRDRMLVMTEALLAAAPTP"
     gene            2241..>2851
                     /locus_tag="VGP27_02020"
     CDS             2241..>2851
                     /locus_tag="VGP27_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7850124.1"
                     /translation="MKILFVMVDGGGNIPPQLAVARTLKSRGAQVHVLGHRGIRERVE
                     AAGLPFESFTEGTHFDPTIQRSLAAIMTAFTRVAADRRLGQCVVEVARRHKVDAVVVD
                     MILTAGIPTVVGAGIPTAVFVHCFYRAVQDLAAGPVGWMLRLRGIDPLAAEHRGLLQV
                     VAARADLDPMRAAPPVCHTGVVWQGVPSAAVPAPVPRILVSLST"
BASE COUNT          533 a          942 c          914 g          462 t
ORIGIN      
        1 gtgccgcagc cggtcagcat ggccgctgcg gcaagcagcg ccggccacag gctcgagatg
       61 cgcctggaca tggtcataga tccgacgaca gcgtcgctgt gagtagtttc tcggcgtcgg
      121 cgcaggggtc gctgtcggtc ctgccgcgcg tctgcaccca ccaactcagc acaccggggc
      181 ctgctgcggc ggtcgccgag caacccgcgc cattcgtatc gcggcgggcc agaaacgcct
      241 cgtgccgctc gacgactttc ccggtgacct ttgcgccgcg ctgatccgca aaggtccgtt
      301 cccggtccag gctgccggtc tcgaaccagg agaagacgac gtcgatcgtg gtgttcggag
      361 gtgcaccggc gccggcatcg ggcccaggac cgcgctccaa cacgtattgg cagacggcgc
      421 cactgtaggg cctcaccacg ttggtggcgc ccaatatctg ctgaatcgag ctgtcctcaa
      481 gtaggccgca tcggttgtcc aagtatccgt agctgcgctg cgggtcgggc accgaagggt
      541 gggctctctg cgcggtcccg ctgacggtct gcgcacatcc cgcgacggtc atcaccgccg
      601 tgaccgccgc gatagcaacc gctttgacag gccaacgcat cgcctgcaca cgctacccag
      661 cggtgtaccg cgccgcgact cgttcggcgt agctccgggg tgaaccgaac cggtcaagtg
      721 ctgccatgga ccactcgcac gacgtacgct ctacagccat gacggctggg ggttgggaag
      781 cgttgagccg atcgatggtt gccttcgcgg tcgcagtgcc cgtcgcgctc accgtgagtg
      841 tgcccgcggc gcaggccaag aacggcgata cgcacatcac ctcgatcggc ggcaatcaga
      901 ccatcgactg caacgagtcg acgctgatcg tgctcggagc caacaacacc atcaacgcgc
      961 tgggcacctg ctgggcggtg acggtgcagg ggtcaggaaa caccatcgtg gccgacaacg
     1021 tgatcaacga catcacggtg tacggcttcg accaggctgt cttctacaaa aacggcgaac
     1081 cggccgtctg ggatcgcgga cgggaacttg gcatgacgaa ccggatcgac cgcattcccg
     1141 cgtgaccgtc cgcgttctgt tcgcaccaaa cggaagcgag aggaacccat gcgtacgcgc
     1201 gtcttgattg gtgtcactgc ggccgccgcg gccgtcgggc ttgccggttg cggagcggaa
     1261 agcagcgaca gcaacactcc gacagcgaca ctcggctccg ggggcggtca ggtcgagatc
     1321 ggcaacacca tcaactacag ctccgtcggc accacgaccg aactcgactg cgcggacggc
     1381 aaatcgctca acatcggcgg caacaacaac aagctgacca tcaagggcac ctgtgccaac
     1441 gccaacatcg gcggcgcgga caacacggtc accatggacc gcgtcgacaa gggactcagc
     1501 gtcgtcggct tcaacaacac cgtcacttac aaacagggtg atccgaagat caacgacacc
     1561 ggctcgggga acaagatcag caagggctga tcggccgtac tttcgtgtct atagctatgg
     1621 acacgaaagc tgaaatcggt gtacattgcc gtggacatga aatcgcgcaa atacgacatg
     1681 ggcgcacgtc agcgggccaa aagcgcgacc cgagacgcga tcatcaaggc cgccattgac
     1741 acgttcatgg ccgaacgttc gttcgcgctc acgctgccga cggtcgccga gcgcgccgat
     1801 gtgaccgtga agacggtgct gcggcatttc ggaagccgcg acggcctcat cgaggccgcg
     1861 tggtcgcagg cctacgacga ggtgatggcc gaacgcatac cgccgccagg cgacctcgag
     1921 ggcgcgctga aggtgctcat cgctcactac gaggcgcgcg gtgaggtcgt gctcgccatg
     1981 ctgaccgaag aaaacgaccc gcgcgccgcc cggatgaaca gtgcgggacg gcttgggcat
     2041 cgtcattggg tcgaagaggt gttcgccgac cgactccccg ccggaaccga cgaacgcacc
     2101 cggttcatcg atgtcctcgt cgtcgccacc gacgtctaca catggaagct gctgcggctc
     2161 gaccgcggtc tgtccgtcga cgacgtgcgc gaccggatgc tcgtgatgac tgaggcgctt
     2221 ctcgccgcgg ccccaacgcc atgaaaatcc tcttcgtcat ggtggacggc ggtggcaaca
     2281 ttccaccgca gttggcggtc gcccggacgc tgaagtcccg tggcgcgcaa gtccacgtac
     2341 tcggacaccg cggtatccgc gagcgcgtcg aagccgcagg tctgccgttc gaatcattca
     2401 ctgagggcac gcatttcgac ccgacgatcc agcgatcgct cgcggcgatc atgacggcat
     2461 tcacccgggt ggcggcggat cgacgactcg gtcaatgcgt ggtggaggtt gcgcggcgcc
     2521 acaaggtgga cgccgttgtc gtcgacatga tcctgactgc gggaatcccc acagtcgtcg
     2581 gtgcaggcat tcctacggcg gtcttcgtgc actgcttcta ccgggcggtc caggacctcg
     2641 cggccggtcc ggtcggatgg atgcttcgac tgcgcgggat agacccgctc gcggccgaac
     2701 accgcggatt gctgcaagtg gtcgccgccc gcgctgatct cgacccgatg cgtgccgctc
     2761 cgccggtgtg ccataccggc gtcgtatggc aaggcgtgcc gagtgccgcc gtgccggcac
     2821 ccgtgccgcg aatcctggtg agcctcagca c
//
LOCUS       DASYLX010000083         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3185635,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000083 DASYLX010000000
VERSION     DASYLX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185635"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGP27_02025"
     CDS             complement(<1..528)
                     /locus_tag="VGP27_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850125.1"
                     /translation="MIANAAFGDEPGRWPLPAARSAEELWQRAVVAGGQGRYARAYAD
                     LASVRRTHRVGRLASLAHSTHASFLRQLGWHAKARGWDGRALALADGDPEAFGDALIG
                     LAADALGVGRFAASATALRRAEGLLGDGPSRLAVRLSWVSAELAMATGEGAAAVRHAE
                     RAVESAAAFGSVRHSA"
     gene            957..1247
                     /locus_tag="VGP27_02030"
     CDS             957..1247
                     /locus_tag="VGP27_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7850126.1"
                     /translation="MPQPQQLPGPNADIWDWQMHGLCRGVDSSVFFHPDGERGRARAQ
                     REMRAKEMCRSCPVITQCRSHALAVGEPYGIWGGLSESEREMLLKRGIRRAS"
     gene            complement(1316..>2293)
                     /locus_tag="VGP27_02035"
     CDS             complement(1316..>2293)
                     /locus_tag="VGP27_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV7850127.1"
                     /translation="RELAAIVTAPHPYKRWIATLAWGALAFATAGTLGAGLLVCVVSA
                     LSAMAIDRVNRRLNRYGLPFFFQYAVGGAIATAPPILLYALGPKLGLHFEPTVAIAAG
                     LVVLLAGLSLVGSVGDVITGAPVTAAARFFELVMLTAATIAGVAFVLHIASRFGAPFV
                     AISASPPPALAELPARVAFGAASAAAFALACYAERSAAMAAAFGGAAGTIAFLLAQST
                     GLGAVVASFVAAVPIGLVGRLMERRNLAPPLVVSIAGIVPLLPGLSLLHGIYAVLNDQ
                     HAVGFASVLGALAIGTALAAGVTLGEWGSWKVRRRRGAFRRRTSRAAAR"
BASE COUNT          416 a          769 c          781 g          327 t
ORIGIN      
        1 cgcgctgtgc cggaccgagc cgaaggctgc ggccgattcg acggcgcgct cggcgtgacg
       61 gaccgcggcc gcgccctcgc cggtggccat cgccagttcg gccgacaccc acgacagccg
      121 gaccgccagc cgcgacggcc cgtcgccgag caaaccctcc gcccgccgaa gagcggtcgc
      181 ggaggcggcg aaccgcccga cgccgagcgc gtcggcggcc agaccgatca gggcatcgcc
      241 gaaagcttcg ggatcgccgt ccgcgagtgc gagcgcgcgg ccgtcccagc cgcgggcctt
      301 ggcgtgccaa ccgagctgcc gcaagaacga cgcatgggtg ctgtgcgcca gcgacgccag
      361 tcgcccgacg cggtgagttc gccggactga agccagatcg gcgtaggcgc gggcgtagcg
      421 gccctgcccg ccggcgacga ccgcacgctg ccacaactcc tcggccgagc gggccgcggg
      481 cagcggccat cgtcccggtt cgtcgccgaa ggcggcgttg gcgatcatca gccgccgacg
      541 ctatcaatag cgtgcggtta acccggtacc ggcgggcacg gataattttt cgacgcagaa
      601 tctctttggt taacagtttg tgcgccacgt gttacagcca tgtcaacggc ctactgggca
      661 ctagacgaaa tcgccgcgat gccgcagaga gcgaactgcg gaaacacgag ccatcgtggg
      721 taaccgagcc attggctatc accctcttcg gtaagcgggg gtcaaaccta tgaacgtgat
      781 gtaaattctc ggcctgcgct tatgtccttg agcacatgtt cgggctattg actcattttg
      841 ctaagcgccc ctaccttgtg tgcacgagta gtcatcggcg acttgtgctc ggatcggttc
      901 cacaactcac gcgcccgtgc ggtccccaaa tcggtgcgta gagaggggtt ttccacatgc
      961 cacagccgca gcagctaccg ggacccaacg cagatatctg ggattggcag atgcacgggc
     1021 tttgccgagg cgtcgattcg tcggtgtttt tccaccccga tggggagcgt ggccgggcac
     1081 gcgcgcaacg cgagatgcga gccaaggaga tgtgccgaag ttgcccggtg atcacgcagt
     1141 gccgctctca tgcccttgcc gtgggtgagc cgtacggcat ctggggcggt ctgtcggagt
     1201 ccgagcgaga aatgctactc aagcgaggca tccgccgcgc ctcataagcc aggaaacgag
     1261 aaggctcccg gacactcgga tgtgtcgggg ggctttttcg tttactgggc agtcgtcagc
     1321 gtgctgctgc ccgcgacgtt ctgcgtcgga acgcaccgcg gcgcctgcgc accttccacg
     1381 agccccattc gccaagcgtg acgccggctg cgagcgcggt accgatcgcg agcgcaccca
     1441 acaccgaggc gaatccgaca gcgtgctggt cgttgaggac ggcgtagata ccgtgcagca
     1501 gcgaaaggcc cggcagcagc ggcacgatgc ccgctatcga caccaccaac ggcggcgcca
     1561 gattgcggcg ttccatcagc ctgccgacca ggccgatcgg cacggcggcc acgaacgacg
     1621 cgaccacggc acccaagccc gtgctctgtg ccagaagaaa cgcgatggtg cccgcggcgc
     1681 caccgaatgc cgccgccatg gccgccgacc gttcggcata gcaggccagg gcgaaggcgg
     1741 ccgcgctggc agcgccgaac gcgacacgtg cgggcagctc ggccagcgcc ggcggtgggg
     1801 atgcgctgat cgcaacgaat ggagccccga agcggctggc gatgtgaagt acgaacgcga
     1861 cgccggcgat cgtcgccgcc gtcagcatca cgagctcgaa aaagcgggcc gccgctgtga
     1921 cgggggcgcc ggtgatgaca tcgccgaccg agccgacgag cgataagccg gcgaggagga
     1981 cgaccaggcc cgctgcgatc gcaaccgtcg gttcaaagtg caggcccagc ttcgggccga
     2041 gtgcgtagag caggatcggg ggcgcggtcg caatcgcgcc gccgaccgcg tactggaaga
     2101 agaacggcag gccgtacctg ttgagacgcc gattgacgcg gtcgatcgcc atggcgctca
     2161 acgcactgac cacacagacc agcaggcccg cgcccagcgt tcccgcggtc gcgaacgcca
     2221 gcgcgcccca ggcgagggtg gcaatccacc gcttgtacgg gtgcggcgcg gtgacgatgg
     2281 cggcaagctc tcg
//
LOCUS       DASYLX010000084         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_772593,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000084 DASYLX010000000
VERSION     DASYLX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772593"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VGP27_02040"
     CDS             <1..729
                     /locus_tag="VGP27_02040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850128.1"
                     /translation="VFLPTMQFSWNFGNYSDAFSRYQDQILRSFLYAFVATVVCLLLA
                     YPLAYVIAFKAGRYKNLILGLVILPFFVTFLIRTIAWKTILADDGWVVSALDSLHLLP
                     SDGRLLSTSWAVIGGLIYNWIIFMILPLYVSLEKIDPRLIEATKDLYSSNTRSFTKVI
                     MPLSMPGVLAGSLLVFIPAAGDFINAEYLGSTQTTMIGNVIQQQFLVVKDYPAAAALS
                     MVLMVIILVGVLAYTRALGTEDLV"
     gene            726..1586
                     /locus_tag="VGP27_02045"
     CDS             726..1586
                     /locus_tag="VGP27_02045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850129.1"
                     /translation="MTTQAGAAVANVAEKADKAPKTVKGSPKWGDVILRVIAGLVLLY
                     LFLPIFVIILFSFNNPKGKFNYSWQGFTLKNWLDPFKYPALTDALKLSINVAAVSTAI
                     ALVLGTLVAIALVRQRFRGSGAVDTFLVLPLTAPEVVMGASLLVLFLDLGWATGYVTI
                     LLAHIAFEVSFIAMTVRARMRGFDWTLEDASMDLGANPARTFFKVTLPLIVPGIIAAA
                     MLSFALSLDDFIITYFVSGSSVTYPLYVNASVKASVPPQINVLATAILLISLVLLGVG
                     TLYRRKRLDA"
     gene            complement(1593..1946)
                     /locus_tag="VGP27_02050"
     CDS             complement(1593..1946)
                     /locus_tag="VGP27_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7850130.1"
                     /translation="MARGIFDTPVAGIVNRAFVALMNAPGIGRLIGRGLVVIRYTGRR
                     SGKTFETPVGYRRSGNTLTINVVAPDAKNWWRNFLGEGGPITFVKLNGRDQNGHAVAE
                     RDASGRVSVTVRLDG"
     gene            complement(2018..2887)
                     /locus_tag="VGP27_02055"
     CDS             complement(2018..2887)
                     /locus_tag="VGP27_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7850131.1"
                     /translation="MTDNQARTGAFMAMGSMACVQVGLAIAVTLIDRIGVEGAAWLRL
                     AWAGVLMLVIVRPRPAAFTRKTFLVCVVLGVVTAMVTMLFMASAGRIPLGTASALEFL
                     GPLGVAVANGKGGGRVLWPGLAAIGVVLLTQPWAGAVDPIGVLYALGAAACWACYILL
                     TQRVGDEVAGINGLAVSMPVAGVVATIVVGPQVLPRMTPDILLIGIGLAILLPVVPFA
                     LELMALRRLNTAAFGTLMALEPAFAMIVGALILHQEPGLAGVVGICLVVAAGVGAART
                     GSRKPTPVPAEVG"
     gene            2971..>3233
                     /locus_tag="VGP27_02060"
     CDS             2971..>3233
                     /locus_tag="VGP27_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850132.1"
                     /translation="METWELAIRESVRQTLADYTAGTDRNRIDDVAACFAPNGALTFT
                     GGEPMVGPTAIVAGLKAQVSRFAQNPVPLTHVRHHVSSMRFGSV"
BASE COUNT          580 a         1070 c          993 g          590 t
ORIGIN      
        1 gtattcctgc ccacgatgca gttctcgtgg aacttcggta actactccga cgcgttcagc
       61 cggtatcagg accagattct gcggtcgttc ctgtatgcct tcgttgccac ggtggtgtgt
      121 ctgctgctgg cctacccgct ggcgtatgtc atcgcgttca aggcgggccg atacaagaac
      181 ctcatcctcg ggctggtgat tctcccgttc ttcgtcacct tcttgatccg caccatcgcg
      241 tggaaaacga ttctggccga cgacggctgg gtggtgagcg cccttgactc gctccacctg
      301 ctgccaagcg acggccgact gctgtcgacc agttgggcgg tgatcggcgg cctgatctac
      361 aactggatca tcttcatgat cctgccgctc tacgtgagcc tcgagaaaat cgatccccga
      421 ttgatcgagg cgacaaagga tctgtactcg tcgaacaccc gcagcttcac caaggtgatc
      481 atgccgctgt cgatgccggg cgtcctggcc ggaagcctgc tggtgttcat ccccgccgcg
      541 ggcgacttca tcaacgcgga gtacctgggg agcacgcaga cgacgatgat cggcaacgtc
      601 atccagcagc agttcctggt ggtcaaggac tacccggcgg ccgccgcgct cagcatggtc
      661 ctgatggtca tcattcttgt cggcgtgctg gcgtacaccc gtgcgctggg caccgaggac
      721 ctggtatgac gacgcaggcc ggtgccgcgg tggcgaacgt ggccgaaaag gcggacaagg
      781 ccccgaaaac ggttaaggga agcccgaagt ggggcgacgt catattgcgg gtcatcgccg
      841 gcttggtgct gctctacctg ttcctgccga tcttcgtcat catcctgttc tcgttcaaca
      901 acccgaaggg caagttcaac tacagctggc agggcttcac actcaagaac tggctcgacc
      961 cgttcaagta cccggcactc accgatgcat tgaagctcag tatcaacgtc gcggccgtat
     1021 ccaccgcgat cgcgctggta ctcggcacgc tggtggccat agccctcgtc cggcaacggt
     1081 ttcggggtag tggggccgtc gacaccttcc tggtgctgcc gctgaccgca cccgaggtcg
     1141 tgatgggtgc atcgctgctg gtgctgttcc ttgacctggg ctgggccact ggatacgtga
     1201 cgattctgtt ggcacacatt gccttcgaag tgagcttcat cgcgatgacc gtacgggcgc
     1261 gcatgcgcgg attcgactgg acgctcgagg acgcatcgat ggatctgggc gcgaacccgg
     1321 cacgcacgtt cttcaaggtg acgcttccgc tgatcgtgcc cggcatcatc gctgccgcga
     1381 tgttgtcgtt cgcgctgtcg ctcgacgact tcatcatcac ctacttcgtc agcgggtcgt
     1441 cggtgaccta tccgctctac gtcaacgcct cggtgaaggc gtcggtgcca ccgcagatca
     1501 atgtgctcgc caccgccatc ctgctcatca gcctcgtcct gctcggtgtc gggacgctgt
     1561 atcggcgcaa gaggctggac gcctgagcgg cctcaaccgt ccagacgaac ggtcaccgaa
     1621 acgcggccgc tcgcgtctct ttcggcgacc gcgtgcccgt tttggtctcg gccgttcagc
     1681 ttcacaaatg tgatcgggcc gccctcgcca aggaagttgc gccaccagtt cttagcgtcg
     1741 ggtgcgacga cgttgatggt cagcgtgttg cccgagcgtc gataacccac cggggtctcg
     1801 aacgtcttgc cggagcgacg gccggtgtag cggatcacga ccagcccgcg gccgatcagc
     1861 cgcccgatgc ccggtgcgtt catgagcgcg acgaacgccc tgttgacgat gccggctacc
     1921 ggcgtgtcga agatgcccct tgccatgtcg tccaacctac caacgagctt cgtagccgtt
     1981 gtcgcactcg tcagtcgccg ccgcgattcc cgatggctca gccgacttcg gcgggcaccg
     2041 gtgtcggttt tcgtgatccc gtgcgcgccg cgccgacacc ggcggcgacc accagacaga
     2101 tgccgacgac gccggccaag ccgggctctt ggtgcaggat cagcgcgccc acgatcattg
     2161 cgaaggcggg ctcgagcgcc atcagtgtcc cgaacgcggc cgtgttcaaa cggcgcaacg
     2221 ccatcaactc gagcgcgaac ggaacaacgg gcaacaggat cgccagaccg atgccgatca
     2281 agaggatgtc cggggtcatc cggggcagta cctgaggccc gacgacgatc gtggccacca
     2341 cgcccgccac cggcatcgac accgccagcc cgttgatgcc ggccacctcg tcgccgaccc
     2401 gctgggtcag caggatgtag caggcccaac atgccgccgc gccaagggcg tacagcacgc
     2461 cgatcgggtc gacggcgccc gcccaaggct gggtgagaag tacgacgccg atcgcggcga
     2521 gaccgggcca cagcacacga cccccaccct tgccgttggc gacggcgaca cccaacggtc
     2581 cgaggaactc cagcgcgctg gccgtgccca atgggatccg acccgccgag gccatgaaga
     2641 gcatcgtcac catggcggtc accacgccga gcaccacaca caccaggaag gtctttctgg
     2701 tgaacgccgc gggccggggc cgaacgatga cgagcatcag caccccggcc caggccagcc
     2761 gcagccaggc cgcaccctcg acgccgatgc ggtcgatgag cgtgaccgcg atcgccagac
     2821 cgacctgcac acacgccatc gaccccattg ccatgaaggc gccggtgcgg gcttgattgt
     2881 cggtcacatc tgcattgaac ggcagcgaga ccgttcacgt ccacgtgatt tttctggata
     2941 tattgttcaa taaccgtgaa cgatagaggc atggagacct gggaattggc gatccgcgag
     3001 tccgtccggc agacgctggc cgactacacg gcgggcaccg accgcaaccg aatcgatgac
     3061 gtcgcggcgt gcttcgcgcc gaacggtgcc ctcacattca caggcgggga accgatggtc
     3121 ggaccgacgg ccatcgtggc agggttgaaa gcgcaggtga gccgattcgc ccagaatccg
     3181 gtgccgttga cccatgtgcg ccatcacgtt tcgagcatgc gattcggttc cgt
//
LOCUS       DASYLX010000085         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2856940,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000085 DASYLX010000000
VERSION     DASYLX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856940"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VGP27_02065"
     CDS             complement(<1..1324)
                     /locus_tag="VGP27_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV7850133.1"
                     /translation="MLAGISLGSDFKRFTRGTMPRIALVTIVVLPLLYGAMYLWAFWN
                     PFGHVDKLPVAVVNDDRGAVAQGRELDAGEQVVRGLVASKQLDLHQVSDNEAAEGVAH
                     GAYYFSITVPEDFSDAIASAAGEHPRKAQIRFTFNDVNNYLASVIGQNTAREVLNQVN
                     QQVGAQTVDAVLVGLTDAGQGLRQAADGAGRLSAGLTTAHDGAQQLAAGSRALADNLA
                     VARDGAATLAAGTRQLSSSITTATDPVVSVLDRIDGLGLDPAAVGETASHLSAAARSA
                     GDRIAALNIDYTQAAGIVDQVVAGLRTNPDPTVRELGETLAGAQSLMRARGIDPTTDD
                     GLVRLRSDAQQLETELGDPNSQLRRLMTRVLDGGLKADVVRLRDGATQLNTGAQRLSG
                     GLVQLTDGGQRLADGSQALVSGTGQLKAGAQVLATKLSEGARQLPSWTV"
     gene            complement(1327..2037)
                     /locus_tag="VGP27_02070"
     CDS             complement(1327..2037)
                     /locus_tag="VGP27_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850134.1"
                     /translation="MAADHRDPQAIVVRGVTLRGPWGPVYGPVDLEADAGGVSVLVCP
                     AGLGRTALLMTLAGRMKPRTGSLTVLGQDAAPAIFARSALAGIDELDGIAEWITVRDA
                     VDEELRWAAPWYRAVPRAGSRELELLCAAVFGPLPMPPLDAYVDGLPELDQLLLRIAL
                     ANTKRPPLLVVANLDRVTDDRHRELLVARLVELGRAQTVITASANGVSPESGVRAQIR
                     VENVGPAEFAAGGQKGAS"
BASE COUNT          382 a          927 c          783 g          410 t
ORIGIN      
        1 gcaccgtcca cgacggcaac tgccgcgctc cctcgctcaa cttggtcgcc aacacctgcg
       61 ccccggcctt cagctggccg gtgccagaca ccaacgcctg cgagccgtcg gccagtcgct
      121 gcccgccgtc ggtcaactgc accagcccgc cgctgagcct ctgcgcgccg gtgttcagct
      181 gtgtcgcacc gtcacgcagc ctgaccacgt ctgccttcag cccgccgtcg agcacccggg
      241 tcatcagcct ccgcagctga ctattcgggt cgcccagttc ggtctccaat tgctgtgcgt
      301 cgcttcgtaa tcggaccagc ccgtcatcgg ttgtcggatc gattcccctg gctcgcatga
      361 gcgactgcgc gcccgcgagg gtctctccca gctcacgcac ggtgggatcc gggttggtgc
      421 gcagcccagc cacgacctgg tcgacgattc ccgcggcttg cgtgtagtcg atgttcagcg
      481 cggcgatccg gtcacccgcc gagcgggccg ccgcgctgag gtgcgacgcc gtctccccca
      541 ccgccgccgg atccaggccc aggccgtcga tccggtccag cacactgaca accgggtcgg
      601 tcgccgtggt gatcgacgac gacaactgcc gcgtccccgc cgccagcgtc gccgccccgt
      661 cgcgcgccac cgccagattg tcggccagcg cgcgcgaccc ggccgccagc tgctgcgccc
      721 cgtcgtgggc tgttgtgagg cccgccgaga gccgcccggc gccgtcggcc gcctgcctca
      781 atccttggcc cgcgtcggtc agcccgacca gcaccgcgtc caccgtctgc gccccgacct
      841 gctggttgac ctggttgagc acttcgcgcg ccgtgttctg cccgataacc gatgccaggt
      901 aattgttgac gtcgttgaac gtgaagcgga tctgcgcctt ccggggatgc tcgccggccg
      961 ctgacgcgat cgcgtcgctg aagtcctcgg gcaccgtgat ggaaaagtag tatgcgccgt
     1021 gggcgactcc ttcggcggct tcgttgtctg aaacctgatg caagtcaagc tgtttcgagg
     1081 ccaccagacc ccgcacgacc tgttcacccg cgtccagctc ccggccctgc gccaccgcac
     1141 cgcggtcgtc gttgaccacc gcgaccggca gcttgtccac gtggccgaac ggattccaga
     1201 acgcccacag atacatcgcc ccgtacagca gcggcagcac aacgatggtc accagcgcga
     1261 tgcggggcat ggttccacgc gtgaaacgct tgaaatcgct gcccagcgat attccggcca
     1321 acatgatcag ctcgcaccct tctggccgcc ggcggcgaac tcggccggcc cgacattctc
     1381 gacacgaatc tgcgcgcgca cacccgactc cggcgacaca ccgttggcgc tggcggtgat
     1441 gacggtctgc gcccgaccga gttcgacgag acgcgccacc agcagttcgc ggtgccggtc
     1501 gtcggtgacc cggtcgaggt tcgcgaccac cagcagcggc gggcgtttgg tgttggccag
     1561 cgcgatccgc agcagcagct ggtcgagctc gggcagcccg tcgacgtagg catccagcgg
     1621 cggcatcggg agcggcccga acacagctgc gcagagcaac tcgagttcgc gcgacccggc
     1681 gcggggcacc gcccggtacc acggtgcggc ccaccgaagc tcctcgtcga cggcgtcacg
     1741 gaccgtgatc cattcggcga tgccgtcgag ttcgtcgatg cccgccagcg ccgacctggc
     1801 gaagatggcg ggggccgcgt cctggcctag cacggtcagc gatcccgtcc gcggcttcat
     1861 ccggccggcc agggtcatca gcaaggccgt tcggcccagt ccggccgggc acaccaggac
     1921 gcttacaccg ccggcatcgg cctcgaggtc gaccggcccg taaaccggtc cccacggtcc
     1981 gcgcagcgtg acgccgcgga ccacgattgc ctgcggatcc cgatggtcgg ccgccacgtg
     2041 cggcgggtac ccggcccatt gggtctgaac ctcacgatca cttcggatgc cggcggagat
     2101 tctttgtttc gattgtttcg atcagccggg acgtcaccac cacgtggtca cagaactcat
     2161 tagttgcgct tgattgtgaa gcacgtggca gctcagccca caactgcgga gccatgcgtc
     2221 aacgtggaat tcgagttcgg ccagtctcca accgcagaac taacttcacc ggcaccgccg
     2281 ctggctgcat cctgtggaga gaccggggct accgcttcgt gccgttaccc ttgagcatca
     2341 gttgaccggc cgccgcgcca aataccgcgt caacgcataa atgtctggtg catgatcgct
     2401 atcgcgccga ctgccggcgg ggcgcggtcg gcgtggccga ggccgcgaca ttgcgacctc
     2461 gacctggcgc gtgatcggcc gcttaccagc cccggcccca gg
//
LOCUS       DASYLX010000086         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3624755,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000086 DASYLX010000000
VERSION     DASYLX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624755"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGP27_02075"
     CDS             complement(<1..196)
                     /locus_tag="VGP27_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850135.1"
                     /translation="MPGSHSSKWNRRSRGSDRVAELQRWEDSGAIWSVVIRKHDQVTV
                     ALLRCDGGEEVDRFTSDDPRL"
     gene            178..609
                     /locus_tag="VGP27_02080"
     CDS             178..609
                     /locus_tag="VGP27_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850136.1"
                     /translation="MSGSPAPAAAVAAIFVWLGMVLAISFLEAPLKFRAPGVTLQIGL
                     GIGRVVFRALNGCELVLTAVLVVAFVVQPPIIWAAVSAGVAVVALLAQVLLVRPKLAR
                     RSQAVLAGGDGPRSHAHWVYVGFEFVKGAGLLVAGVLLLMA"
     gene            622..1494
                     /locus_tag="VGP27_02085"
     CDS             622..1494
                     /locus_tag="VGP27_02085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7850137.1"
                     /translation="MRLGLHALGIGNGADRAVIDAVAAAADSAGFATLWSGEHVVMVD
                     ESASRYPYSDDGRIAVPAEADWIDPLIGLSFAAAATSTIRIATGVLLLPEHNPVLVAK
                     QAATLDSLSGGRLTLGIGIGWSREEFDALGVPFERRGARTKEYVEAIRTLWRDDVASF
                     DGEFVHFDAVRVNPKPVHDRRIPIVIGGNSDAALRRVAAWGDGWYGFNLDGVDAVAER
                     VAVLRGFCEQQNRDPAELRIAVALRDLTPDDVAPLAASGVDELVLVEGPPEDASVAAD
                     WVRALADHWMTSLG"
     gene            complement(1498..2913)
                     /locus_tag="VGP27_02090"
     CDS             complement(1498..2913)
                     /locus_tag="VGP27_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEV7850138.1"
                     /translation="MHELLEREAKWDVGEDFVLPQLDDLVDGGDIDRSTVHLESVYYD
                     TADRDLQSHGIVLRRRDGDDDIGWQLKVPDVDGRIEIRAPLSDEPPSELKDLLTGLRL
                     GKPLVSVATLRTERTRYRIRQHERLCAELADDHVHASVDHRLLAWREIEVEYESDGQR
                     VSRRLADRLSKAGARPSRYRSKLDHALGAADPPAIDNAALLALTIYVADQIDAVFEGD
                     LQLRRGHDPIHDTRVAIRRLRSTIRVFGKLLDRPATEHLDEELKWFAGLLGEVRDRQV
                     QRKRFREVLDNWPPELVLGPVANRIDSDLHSEQLKARTAVSEAMDSQRYLDVLATLQR
                     WRADPPLATRPTAEKLHKRAAKAERKADRRLSAAIDNDDGALLHRARKAAKRARYAAE
                     LRGPLEKSKAVKKAEKHFKRIQRVLGDHQDSVVASDALRRFALTAGTTPGENGFTYGL
                     LYAREQQIAEAARAQARDLLG"
     gene            complement(2956..4008)
                     /locus_tag="VGP27_02095"
     CDS             complement(2956..4008)
                     /locus_tag="VGP27_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEV7850139.1"
                     /translation="MTDYLKVTAYFGERLRHQDRFLSDALLDLYGEHAVATSIVLRGV
                     ASFGPHHELRSDQSLSLSEDSPIAIAAVDVADKMAGLADQVSAMISRGLVTLERAHLG
                     GSAPPPDAAKLTIYLGRQDRADGRPAHRAVCQVLLRLGFAGASVFLGVDGTAHGQRRK
                     ARFFGRNLGVPVMIIAVGTGRQVTEALPELETLLREPIFTIERAQLCKRDGELLTRPT
                     TLPSTDQHGNPLWQKLMVYTSEATLHDGTPIHRAIVHRLFESRAASGATVLRGVWGFH
                     GDHEPHGDKWLQLGRRVPVTTIVVDTPDRIAASFEIVDELTSKHGLVTSELIPALVSI
                     DGEDRRGGTGLARYEY"
     gene            complement(4005..4370)
                     /gene="crcB"
                     /locus_tag="VGP27_02100"
     CDS             complement(4005..4370)
                     /gene="crcB"
                     /locus_tag="VGP27_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV7850140.1"
                     /translation="MTVAVWTGVVLLGGLGAVCRFLLDRAVTKRVGRPFPFGTLVVNL
                     SGALLLGFFAGLALSPHLALLAGTAFVGSYTTFSTWMLETQRLGEERQVVPAFANIAV
                     SVVLGLAAAWLGQTIGAAL"
     gene            complement(4367..4765)
                     /gene="crcB"
                     /locus_tag="VGP27_02105"
     CDS             complement(4367..4765)
                     /gene="crcB"
                     /locus_tag="VGP27_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV7850141.1"
                     /translation="MFSFDGRELAAVFVGGAIGTLARAGLETLAAPDPGRWPWPTFIV
                     NIVGAFLLGYFVTRLLERLPTSSYRRPLLGTGLCGGLTTFSTMQVETIKMLEHHHYGL
                     AVGYTLASIGAGLLAVYVATAMTRRVGVRR"
     gene            4837..>5928
                     /locus_tag="VGP27_02110"
     CDS             4837..>5928
                     /locus_tag="VGP27_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HEV7850142.1"
                     /translation="MAANPRAGQPAQPQDLIDVAQVVTAYYTVEPDPDNIDQQVAFGT
                     SGHRGSSLDAAFNEAHILATTQAIAEYRKAQGTSGPLFIGRDTHALSEPAWVSALEVL
                     AANDVVAMIDSADRYTPTPAVSHAILSFNRGREGDLADGIVVTPSHNPPRDGGFKYNP
                     PNGGPADTDATGVIAKRANEILRDGLAAVKRVPLARALRTAQRHDYMDAYVADLPNVV
                     DMHAISAEKIRIGADPLGGASVDYWGAIAERHNLELTVVNPLVDATWRFMTLDTDGKI
                     RMDCSSPNAMASLIANRESYQIGTGNDADSDRHGIVTPDGGLLNPNHYLAVAIEYLYT
                     NRPEWPSSTAVGKTAVSSSIIDRVVAGLGR"
BASE COUNT          959 a         1966 c         2001 g         1002 t
ORIGIN      
        1 gcagccgcgg gtcatcagag gtgaaccggt ccacttcctc gccgccgtcg catcgcaaca
       61 gcgcgaccgt cacctggtca tgtttccgga tgaccaccga ccagatggcg ccggagtcct
      121 cccagcgttg aagttctgcg acccgatcgc tgcccctgct tctacgattc cacttcgatg
      181 agtgggagcc cggcaccggc cgccgctgtt gcggcgatat tcgtgtggct gggcatggtg
      241 ctggcgattt cgttcctcga ggctccgctg aaattccgtg cacccggggt gacattgcag
      301 atcggtctcg gcatcggccg cgttgtcttc cgtgcgctca acggctgcga acttgtactg
      361 accgcggtgt tggtggtcgc cttcgtcgtc cagccgccga tcatctgggc ggcagtgtca
      421 gcgggtgtcg ccgtcgtcgc acttcttgca caggtgctgc tcgtgcggcc gaaactggcc
      481 aggcgatccc aagcggtact ggcgggcggc gacggaccgc gctcgcacgc gcactgggtg
      541 tacgtcggct tcgagttcgt caagggcgcg ggtctgctcg tcgcgggcgt tctgctgctc
      601 atggcgtgag acgatcgccc ggtgcggttg ggcttgcatg cgctgggcat cggcaacggg
      661 gccgaccgcg ctgtcatcga cgccgtcgca gccgctgccg attctgcggg gttcgcgacg
      721 ctgtggtcgg gcgaacacgt ggtgatggtc gatgagtcgg cgtcgcgcta tccgtactcc
      781 gacgacggcc ggatcgccgt gcccgcggag gccgactgga tcgacccgtt gatcgggttg
      841 agtttcgcag cggccgccac ctccaccatt cggatcgcga cgggcgtgct gctgctgccc
      901 gagcacaatc cggttctggt ggccaagcaa gccgcgacgc tggactcgct cagcggcggc
      961 aggttgacgc tcggcatcgg catcggctgg tcgcgtgagg aattcgacgc gctcggtgtg
     1021 ccgttcgagc gtcggggtgc gcgcaccaag gagtacgtcg aggcgatccg cacgttgtgg
     1081 cgcgacgacg tggcctcgtt cgacggcgaa ttcgtccact tcgacgccgt gcgcgtgaat
     1141 cccaaaccag tgcacgaccg acgcattccg atagttatcg gcggcaacag cgacgctgcg
     1201 ctgcgccgtg tcgcggcgtg gggcgacggt tggtacgggt tcaacctgga cggcgtcgac
     1261 gcggtggccg agcgggtggc cgtcctgcgc ggtttctgcg aacagcagaa ccgagatccc
     1321 gccgagctac ggattgccgt ggcgcttcgc gacctcacgc cggatgacgt cgcaccgctt
     1381 gccgcatcgg gtgttgacga actcgtgctc gtcgagggcc cacctgagga tgcgagcgtc
     1441 gccgccgatt gggtgagggc gctggccgat cattggatga ccagcctcgg ttagcggtta
     1501 gccgagaagg tcacgcgcct gagcgcgcgc ggcttcggcg atctgctgct cccgggcata
     1561 cagcaggccg tacgtgaatc cgttctcgcc cggcgtcgtt ccggccgtca atgcgaaccg
     1621 gcgcaatgca tccgacgcga cgacgctgtc ttgatgatcg cccagcaccc gctggattcg
     1681 cttgaaatgc ttctctgcct tcttgacagc ttttgatttc tccaacggtc cgcggagttc
     1741 ggccgcgtag cgtgcccgct tggcggcctt gcgggcgcga tgcagcagcg cgccatcatc
     1801 gttgtcaatc gccgccgaga gtcgtcggtc ggccttgcgc tcggccttcg ccgctcgctt
     1861 gtgcaacttc tcggcggtgg gccgggtcgc gaggggaggg tccgcccgcc accgctgcag
     1921 cgtcgccagg acatcaagat agcgctgtga gtccatggct tcggaaactg ccgttcgtgc
     1981 cttcagctgc tcggaatgca ggtcgctgtc tattcgattg gcgacgggtc ccagcaccaa
     2041 ctcgggtggc cagttgtcga gaacctcgcg gaagcgtttg cgctgcactt ggcggtcgcg
     2101 aacctcaccg agcagtccgg cgaaccactt gagctcctcg tcgaggtgtt cggtggccgg
     2161 ccggtcgagc agcttgccga acacccgaat cgtgctgcgc agccgacgga tcgcgaccct
     2221 ggtgtcgtga atcgggtcat gtccccgccg cagctgcagg tcgccttcga agacggcgtc
     2281 gatctgatcg gcgacgtaaa tggtgagcgc gagcagcgcg gcgttgtcga tggcaggtgg
     2341 gtcagcggcg cccagcgcgt ggtccagctt cgacctgtac cgcgacgggc gcgcgcccgc
     2401 cttgctgagc cgatcggcca accggcgaga caccctctgg ccgtccgatt cgtactccac
     2461 ctcgatctct cgccaggcca gcaggcggtg atcgaccgat gcgtgcacgt ggtcatcggc
     2521 tagctctgca cacagacgct cgtgctgccg gatgcggtac cgggtgcgtt cggtgcgcag
     2581 cgtagcaacg ctgaccagcg gcttacccag ccgcagtccc gtcagtaagt ccttcagttc
     2641 agacggcggc tcatccgaca gaggggcgcg gatctcgata cggccatcga cgtcgggaac
     2701 cttgagctgc cagccgatgt cgtcgtcacc gtctcgtcgt cgaaggacga tcccgtgaga
     2761 ctgcaggtca cgatcggcag tgtcgtagta cacgctttcc aggtgcacgg tgctgcgatc
     2821 gatgtcgcca ccgtcgacca gatcgtctaa ttgcggcaga acgaaatcct cgccgacatc
     2881 ccacttggct tcgcgctcga gcagctcatg catgacggag ggattcccgt tcccgatgga
     2941 gagagaaaca tcggatcagt actcatagcg ggccaacccg gtgccgccgc ggcggtcctc
     3001 accgtcgatc gacaccaatg ccggaataag ctcgctcgtc accagcccgt gtttgctggt
     3061 gagttcgtcg acgatttcga agctcgccgc gatgcggtcg ggcgtatcga cgacgatcgt
     3121 cgtcacgggc actcggcgtc ccagctgaag ccacttgtcg ccgtgcggtt cgtgatcgcc
     3181 gtgaaatccc cacacgcccc ttaacaccgt cgcgccgcta gccgcccgcg attcgaacag
     3241 tcggtgcacg atcgcgcggt gaatcggcgt tccgtcgtga agcgtggctt ccgaggtgta
     3301 caccatcagc ttctgccaca acgggtttcc gtgctggtcg gtggagggca gggtcgtcgg
     3361 ccgggtcagc agctcgccgt cgcgcttgca cagttgtgcg cgctcgatcg tgaaaatggg
     3421 ttcccgcaag agggtttcga gttccgggag cgcctcggtg acctgccggc cggtaccgac
     3481 tgcgatgatc atcaccggga cgccgaggtt gcggccgaag aagcgagcct ttctgcgctg
     3541 accgtgcgcc gtcccgtcga cgcccaggaa caccgaggcg cccgcgaacc cgagccgaag
     3601 caacacctgg cacaccgcgc ggtgcgcagg tcgtccgtcg gcgcgatcct gccggccgag
     3661 gtagatggtg agcttggccg catcgggtgg cggcgctgaa ccgccgaggt gtgcgcgttc
     3721 gagtgtgacg aggccgcggg agatcatcgc ggagacctgg tcggcaagcc cggccatctt
     3781 gtcggccacg tctaccgctg cgatcgctat cggtgagtcc tccgacaggc tcagggactg
     3841 gtcgcttcgc agttcgtggt gtgggccgaa gctggcgacg ccgcgcagca cgatgctggt
     3901 ggcgaccgcg tgctcgccat agaggtcgag gagggcgtcg gacaggaagc gatcctggtg
     3961 gcggaggcgc tccccgaagt aggcggtgac cttgaggtag tcggtcacag cgcggccccg
     4021 atcgtctggc ccagccaggc cgcggccagg ccgagtacga cgctgaccgc tatgttggcg
     4081 aacgccggaa ccacctggcg ctcttctccg aggcgctggg tctccagcat ccacgtcgag
     4141 aaggtcgtgt acgagccgac gaacgcggtg cccgccagca gcgcgaggtg agggctgagc
     4201 gcgagcccgg cgaagaaccc gagcagcagc gccccgctga ggttgacgac gagcgtgccg
     4261 aaggggaagg gacggccgac ccgcttcgtc accgcgcggt cgagcaggaa gcggcatacc
     4321 gcgccgagac cgccgagcag cacgacgccg gtccagaccg cgacggtcat cggcgcaccc
     4381 cgactcgccg ggtcatcgcg gtcgcgacgt agacagcaag caagcccgcg ccgatgctgg
     4441 ccagggtgta gccgaccgcg aggccgtagt ggtggtgctc gagcattttg atggtctcga
     4501 cctgcatggt ggaaaacgtc gtcaggcccc cgcacaggcc ggtgccaagg agggggcgcc
     4561 ggtagctcga tgtcggcaaa cgctcgagca gacgggtgac gaaatagccc aacaggaatg
     4621 cgccgacgat gttgacgatg aacgtgggcc acggccagcg gcccgggtct ggcgcggcta
     4681 acgtttccag ccccgctcgg gccaaggtgc cgatcgcccc gccaacgaaa acggcggcca
     4741 gttcgcgccc gtcgaaacta aacacaagcc aaagtaacgc cgtccagctt ccggaccggc
     4801 gtacggcacg gattccggcg gggcacaatc gggcttatgg ccgccaaccc tcgcgccggt
     4861 cagccggcgc aaccccaaga cctcatcgat gtggcccagg tggtcaccgc gtactacacc
     4921 gtcgaaccgg acccggacaa catcgaccag caggtcgcct tcgggacctc ggggcaccgc
     4981 gggtccagcc tggacgcggc gttcaacgag gcgcacatcc tggccaccac gcaggccatc
     5041 gccgagtacc ggaaggcgca ggggacgtcc ggccctctgt tcatcggccg cgacacccac
     5101 gcgttgtccg aaccggcctg ggtgtccgcg ctggaagtgc tggccgccaa cgacgtcgtg
     5161 gcgatgatcg actcggccga ccgctacacg ccgacgcccg ccgtcagcca tgccatcctg
     5221 tcgttcaacc ggggccgcga aggtgacttg gccgacggca tcgtcgtcac cccgtcgcac
     5281 aacccgccgc gcgacggggg attcaagtac aaccctccca acggcggccc ggccgacacc
     5341 gatgcgacgg gtgtgatcgc caaacgcgcc aacgagatac tgcgcgacgg cctggctgcc
     5401 gtgaagcgcg tgccgttggc gcgcgccctg cggaccgcgc agcgccacga ctacatggac
     5461 gcgtacgtcg ccgacctgcc gaacgtggtc gatatgcacg ccatcagcgc cgagaagatc
     5521 cgcatcggcg ccgacccctt aggcggggcc agcgtcgact actggggcgc gatcgccgaa
     5581 cggcacaacc tcgagctgac ggtggtcaac ccgctcgtgg acgcgacgtg gcggttcatg
     5641 acgctggaca ccgacggcaa gatccggatg gactgcagtt cgcccaatgc gatggcctcg
     5701 ttgatcgcca accgcgagtc gtaccagatc gggaccggca acgacgccga ctctgaccgg
     5761 cacggcatcg tcacgccgga cggcggcctg ctgaatccga accactatct ggcggtggcg
     5821 atcgaatacc tctacaccaa ccgtccggag tggccctcgt cgacggccgt cggcaagacc
     5881 gcggtcagct cgtcgatcat cgaccgggtc gtcgccggtc tgggccgc
//
LOCUS       DASYLX010000087         6498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_991830,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000087 DASYLX010000000
VERSION     DASYLX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991830"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..688
                     /locus_tag="VGP27_02115"
     CDS             86..688
                     /locus_tag="VGP27_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEV7850143.1"
                     /translation="MGVPPARRVGGEQNGSSKASTKIVISGGFGVGKTTFVGAVSEIM
                     PLRTEALVTNASAGLDGLEATPDKRTTTVAMDFGRITLDENLVLYLFGTPGQRRFWFM
                     WDDLVRGAIGAIILVDTRRLEDSFAAVDFFEARHMPFLIAINEFDDAASHAPHEVRKA
                     LALSDDVPIITVDARDRQSSTAALIAVTEYALNVLPSVPG"
     gene            695..1255
                     /locus_tag="VGP27_02120"
     CDS             695..1255
                     /locus_tag="VGP27_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV7850144.1"
                     /translation="MEAETVWAEREFVEHDFRDDDLTRLRTERVVFTECDFSGVDMGE
                     SEHAGSAFRNCTFRRTTLWHSAFRHCSMLGSVFTECRLRPVTFVEVDFTLAVLGDCDL
                     RGVDLSDCRLSEAGLVKTDLRKAVLRRADLRGARTQDTRLDEADLRGARIDPTLWTTA
                     SVRAAKIDIEQALAFAAAHGLDVHGE"
     gene            complement(1260..1559)
                     /locus_tag="VGP27_02125"
     CDS             complement(1260..1559)
                     /locus_tag="VGP27_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7850145.1"
                     /translation="MGAAAAAAALLGATTAPASAWPIPLTSEDNNYLGAVRGSWPVDD
                     DTLLMAGRGACRLLYTGSGTQGAIDGTTSLYGASPDQAAVVVRAARGNFCTQAPG"
     gene            complement(1748..4384)
                     /locus_tag="VGP27_02130"
     CDS             complement(1748..4384)
                     /locus_tag="VGP27_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7850146.1"
                     /translation="MSRPAPPALTVRNDGSARTFAAGNDVVIGRDLRADVRIAHPLIS
                     RAHLVLRFDQGRWIAIDNGSLNGMYVNGRRVPTVDIQDGMHVNIGNPDGPELTFEVGR
                     QQGAGRPPTSAIPIANRPSGAWPAQQPPPTQQPPISRPQPYPSGPGPQGYPSTQQPRY
                     PGSPQPSYAGAQAHSTRAPSSVSQQLAPPGLSQPSLESVTAMGPAAAPRSSEGNLATS
                     MLKILRPGRPADTSAPGSIKIGRNTDNDIVIPDVLAGRHHATLVPTAAGTEIRDNRSI
                     NGTFVNGMRVESALLNEGDTVTIGNIDLLFRGGTLVRRTETTAASGTGGLDVHGVTWT
                     IEGNKTLLNDISLTARPGTLTAVIGPSGAGKSTFARLVAGYTHPTSGTVAFEGHDIHA
                     EYASLRSRIGMVPQDDVVHGQLTVNQALMFAAELRLPPDTNKEDRERVVAQVLEELEM
                     TQHRGTRVDKLSGGQRKRASVAMELLTGPSLLILDEPTSGLDPALDRQVMTMLRQLAD
                     AGRVVLVVTHSLSYLDVCDQVLLLAPGGKTAYAGPPSGIGAAMGTTNWADIFSSVARD
                     PDAAAQRFIATHGPTPPRPPAQTPADLGKPTKTSLRKQFSTISRRQMRLIISDRGYFI
                     FLAFLPFIMGVLSLSVPGDVGFGVPVPAIQGGEAPNEPGQILVLLNVGAIFMGTALTI
                     RALIGERAIFLREQAVGLSTTAYLSAKVVVFALFAVMQSAIVVAINIYGKKWGPGAVE
                     SGSVIPNRTLELFVDMAACCVAAAMVGLALSALAKSNEQIMPLLVIAIMSQLVFQGGM
                     IPVTGRIGLDQLSWITPARWGFASTASTIDLLRLVPGPLTPQDSHWKHTPGAWWFNMA
                     MLGCICIGYLSFVRFKIRLKGG"
     gene            complement(4415..5695)
                     /locus_tag="VGP27_02135"
     CDS             complement(4415..5695)
                     /locus_tag="VGP27_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEV7850147.1"
                     /translation="MSPSGEVGDPPPRREGGRIAQPNVFTPAKLGPVTLRNRVIKSAT
                     FEGATPNALVTDDLITYHQLPAAGGVGMTTVAYCAVAEAGRTEPTQIWMRPEAVPGLQ
                     KLTETIHAEGAAVSAQIGHAGPVRHSRVGKTKALAPVRLFNPLVMSFTRKATRDDITE
                     VINAHANAARIAIDSGFDAVEIHLGHGYFCSSFLSPLINRRDDEFGGSLENRAKVARG
                     VVNAVRQAVGDQLAVTAKLNMTDGVRGAIKVEEALQTAKWLEEDGGLDAIELTAGSSF
                     INPMYLFRGDVPLKEFANVFKPPIRWGVRLLGSKVFREYPYRETYLLRDAKLFRAELK
                     MPLILLGGITNRESMDLAMAEGFEFVAMGRALLAEPDLINRIQADGDNHSVRSLCNHC
                     NECMPTALRSSASIHTHCVVTGSPDRRPTSLSPS"
     gene            5749..>6498
                     /locus_tag="VGP27_02140"
     CDS             5749..>6498
                     /locus_tag="VGP27_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV7850148.1"
                     /translation="MGAITLDGKATRDEIFVDLKERVAALTAAGRTPGLGTVLVGDDP
                     GSQAYVRGKHADCAKVGINSIRRDLPADISQAELDQTIDELNANPECTGYIVQLPLPK
                     HLNENAALERLEPGKDADGLHPMNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIAGAQ
                     VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRHLPQLTREADIIIAAAGVPHMVT
                     AEMVRPGAAVVDVGVSRDENGKLVGDVAPDVW"
BASE COUNT         1061 a         2114 c         2238 g         1085 t
ORIGIN      
        1 gaccggtcga gcgtcgacga acgacgtgag ttgataggaa ggaccctccg tggcttacga
       61 gcactctgag ggtcaccgcg cacgcatggg ggtacctccg gcccgaaggg tagggggaga
      121 gcaaaacggc agtagtaagg cgtcgaccaa gatcgtcatc tccggtggtt tcggcgtcgg
      181 taagaccacg ttcgtcggag cggtttccga gatcatgccg ctgcgcaccg aggcgctggt
      241 gaccaatgcc tcggccgggc tggacgggct ggaggccacg ccggacaaac ggaccacgac
      301 ggtggcgatg gacttcggcc gtatcaccct cgacgagaac ctggtgctgt atctgttcgg
      361 cactccaggg cagcgccggt tctggttcat gtgggacgac ctggtgcgtg gcgcgatcgg
      421 cgccatcatc ctggttgaca cccgtcgcct cgaggacagc ttcgccgcgg tggacttctt
      481 cgaggcccgc catatgccgt tcctcatcgc gatcaacgaa ttcgacgacg ccgcaagcca
      541 tgccccgcac gaagtgcgca aggcactcgc actttccgat gacgtaccca tcatcaccgt
      601 cgacgccagg gatcggcagt cctcgacggc cgcgctgatc gccgtcacgg agtacgcgct
      661 caacgtgctt ccgtcggtgc ccggctgatc gcgggtggag gccgaaaccg tttgggcaga
      721 gcgggaattc gtcgagcacg attttcgcga cgatgatctg acccggctgc ggaccgagcg
      781 agtggtgttc accgagtgcg acttcagcgg tgtcgatatg ggcgagtccg aacacgcggg
      841 gtcggccttc cgtaattgca ccttccgtcg tacaacgctg tggcacagcg cctttcggca
      901 ttgcagcatg ctgggctcgg tgttcaccga atgccggctg cggccggtga cgttcgtcga
      961 ggtggatttc acgctggccg ttctcggtga ctgcgacctg cgcggcgtcg acctgtccga
     1021 ctgcagactg agcgaggccg gcttggtcaa gacggacctg cgaaaggcgg tgctgcgccg
     1081 cgcggatctg cgcggcgccc gcacgcagga cacccgactg gacgaggccg acctgcgcgg
     1141 cgcgcggatc gacccgacgt tgtggacgac cgcctccgtg cgcgccgcca agatcgacat
     1201 cgagcaggcg ctggcctttg ccgccgcgca cgggctcgac gtgcacggcg agtgagggcc
     1261 tagcccggcg cctgcgtgca gaagttgccg cgagccgccc gcacgacgac ggctgcctgg
     1321 tcggggctcg cgccgtacag tgacgtggtc ccgtcgatag cgccctgggt ccctgagccg
     1381 gtgtagagca ggcggcacgc cccccgaccc gccatcagca gcgtgtcgtc gtcaaccggc
     1441 caactgccgc ggacggcacc gagatagttg ttgtcctcgg aggtcagggg gatcggccag
     1501 gcggacgcag gcgcggtggt ggctcccagt aacgctgccg ctgccgccgc cgcgcccatc
     1561 aagcgaacag ttcgtctgcg agtggacata gctgtccatc cttcctgtgg cgtgccgaat
     1621 gggacgcggg cacgaatggc atggctccgt atgtccagcc tagcggctgt atagaagtgc
     1681 ccggtacgcc aattctccga cggccacagg ccgagccggt gattagccag cccggcgggt
     1741 cagcacacta accgcccttg agcctgatct tgaaccgcac aaagctcagg tagccgatgc
     1801 agatacatcc gagcatggcc atgttgaacc accacgcgcc cggagtgtgc ttccagtgcg
     1861 agtcctgcgg ggtcagcggg cccggtacga gccgcaggag atcgatcgtc gacgcggtcg
     1921 aggcgaatcc ccaccgcgca ggcgtgatcc atgacaactg gtcgagaccg attcggccgg
     1981 tcaccgggat catgccgccc tggaacacca gctgcgacat gatcgcgatc acgagcagcg
     2041 gcatgatctg ctcgttcgat ttggccagcg ccgagagcgc cagaccgacc atcgccgcgg
     2101 ccacgcagca ggccgccatg tcgacgaaca gctcgagggt ccggttgggg atcaccgagc
     2161 cgctctccac ggctcccgga ccccacttct tgccgtagat attgatggcg acgacgatgg
     2221 ccgactgcat caccgcaaac agggcgaaga cgacgacctt ggccgaaagg taggcggtcg
     2281 tggacaaccc gacggcctgc tcacgcagga agatggcccg ctcgccgatc agagcgcgga
     2341 tcgtcaaggc cgtgcccatg aagatggcgc cgacgttgag cagcacgagg atctgccccg
     2401 gctcgttcgg cgcctcacca ccctgtatcg caggaacggg cacgccgaag ccgacgtcgc
     2461 ccggcaccga caacgacagc acacccatga tgaacggcag aaatgccagg aagatgaagt
     2521 acccgcggtc ggagatgatc agcctcatct ggcgacgaga gatcgtcgag aactgcttcc
     2581 gcaggctggt ctttgtcggt ttaccgaggt ccgccggcgt ctgagcgggc ggccgtggtg
     2641 gcgtcggccc gtgcgtggcg atgaatcgtt gggcggccgc gtcggggtcg cgcgcgaccg
     2701 aactgaagat gtcggcccag ttcgtcgtgc ccatcgccgc gccgatgccg ctgggcggac
     2761 ccgcgtacgc ggtcttgccg ccaggggcca gcagcaacac ctgatcgcag acgtcgaggt
     2821 aggacaacga gtgggtgacc acgagcacca cgcggcccgc gtcggcgagc tggcgcaaca
     2881 tcgtcatgac ctggcggtcc agcgcggggt cgaggcctga tgtcggctcg tcgaggatca
     2941 gcaatgaggg acccgtcagc agctccatgg ccaccgatgc gcgtttgcgc tggccgccgg
     3001 acagcttgtc gacgcgggtt ccgcggtgct gcgtcatctc gagttcctcg agcacctgtg
     3061 cgacgacccg ttcacggtct tccttgttgg tgtccggcgg cagccgcagt tcggcggcga
     3121 acatcagagc ctggttgacg gtcagctggc cgtgcaccac atcgtcctga ggcaccatcc
     3181 cgatccggga gcgcagcgac gcgtactcgg cgtggatgtc gtgtccctcg aaggccaccg
     3241 tgcccgacgt cgggtgggta tagcccgcca ccaatcgcgc gaaggtggac ttgcctgcgc
     3301 ccgatgggcc gatgacggcg gtcagcgtgc cgggccgcgc cgtcagcgag atgtcgttca
     3361 gcagcgtctt gttgccctcg atggtccacg tgacgccgtg cacgtccagg ccgccggtgc
     3421 cgctcgccgc ggtggtctcg gtgcggcgca ccagcgtgcc gccgcggaac agcaggtcga
     3481 tgttgccgat ggttactgtg tcgccctcgt tgagtaaggc gctctcgacg cgcatgccgt
     3541 tgacgaacgt cccattgatg ctgcggttgt cgcggatctc agtgcccgcg gcggtcggca
     3601 ccagggttgc gtggtggcgg cccgccaaca cgtcggggat gacgatgtcg ttgtccgtgt
     3661 tgcggccgat cttgatcgac cccggcgccg aggtgtccgc aggccgaccg ggtcgcagga
     3721 tcttcagcat gctcgtcgcg aggttgccct cgctcgagcg gggcgcggcc gccgggccca
     3781 tcgccgtgac cgactcgagg gaaggctgcg acagccccgg cggtgcaagc tgttgcgaca
     3841 ccgaactcgg tgcgcgggtg gagtgtgcct gcgccccggc gtagctcggc tgcggggagc
     3901 cgggataccg gggctgctgg gtcgacggat agccctgcgg acccggaccc gacgggtacg
     3961 gctgcggccg cgagatgggc ggctgctgcg tgggcggcgg ctgctgcgcg ggccaggctc
     4021 cgctcgggcg gttcgcgatg ggaatcgccg atgtcggtgg cctgcccgca ccctgctgcc
     4081 tgccgacctc gaaggtgagc tcggggccgt cggggttgcc gatgttgacg tgcatgccgt
     4141 cctggatgtc gacggtcggt acccggcgtc cgttgacgta catgccgttg aggctgccgt
     4201 tgtcgatggc gatccaccgg ccctggtcga aacgcagcac caggtgtgcc cgggagatca
     4261 acgggtgtgc gatgcgaaca tcagcgcgaa gatcgcgacc gatgacgaca tcatttccag
     4321 cggcgaatgt gcgtgctgat ccgtcattcc gaacggtcag cgcgggcggg gctggtcggc
     4381 tcatcgcgac caactctata ggtatccgct cgttctacga cggggatagg gaggtcggac
     4441 gtcggtccgg cgacccggta accacgcagt gggtgtggat ggacgcgctc gaccgcaggg
     4501 ctgtcggcat gcactcgttg cagtggttgc agagagaccg caccgaatgg ttgtcgccgt
     4561 cggcctggat ccggttgatc aggtcgggtt cggcaagcaa cgccctgccc atcgcgacga
     4621 actcgaaacc ctccgccatc gcaaggtcca tcgactcgcg gttggtgatg ccgccgagca
     4681 ggatcagcgg catcttcaac tctgcgcgga acagtttggc gtcgcgcagc agatacgtct
     4741 cgcggtaggg atactcgcgg aacaccttgc tgccgagcag ccgcaccccc caccggatcg
     4801 gcggcttgaa gacattggcg aactccttga gcggcacgtc accgcgaaac aggtacatcg
     4861 ggttgatgaa cgaactgccc gccgtcaact cgatcgcgtc gagcccaccg tcctcctcga
     4921 gccacttggc cgtctggaga gcctcttcga ccttgatggc gcctcgcacc ccgtctgtca
     4981 tgttgagttt cgccgtcacc gcgagctgat cgccgaccgc ttgcctgacg gcgttgacca
     5041 ccccgcgcgc caccttcgcc cggttctcca gcgacccgcc gaactcgtcg tcgcgacggt
     5101 tgatcagcgg gctcaaaaaa gagctgcaga agtagccgtg tcccagatgt atctcaacgg
     5161 cgtcgaatcc cgaatcgatg gcgatccgcg cggcgttggc gtgtgcgttg atgacctcgg
     5221 tgatgtcgtc gcgggtggcc tttctggtga agctcatcac caggggattg aagaggcgta
     5281 ccggcgccag cgccttggtc ttgccgacgc gcgaatgcct gaccggtccg gcgtgtccga
     5341 tctgcgcact gaccgccgcg ccttcggcgt gaatggtctc ggtgagcttt tgcaaaccgg
     5401 gcaccgcctc ggggcgcatc cagatctggg tgggctcggt gcggccggcc tccgcgaccg
     5461 cacagtaggc gaccgtcgtc atgccgacgc cgcctgccgc aggcagctgg tgataggtga
     5521 tcaagtcgtc ggtgacgagc gcgttcgggg tcgctccctc gaaggtcgcc gacttgatga
     5581 cgcggttgcg cagagtcacc gggccgagct tggccggggt gaacacattg ggctgggcaa
     5641 tccgtccccc ttcccttcgg gggggagggt cccccacttc acccgagggg ctcatcgccg
     5701 cgtccatggg tcagaacact gccacagtgc cgtgtcagac tggctgccgt gggtgcgatc
     5761 actttggacg gtaaggccac acgcgacgag atcttcgtcg atctcaagga gcgggtggcg
     5821 gccctgaccg ccgccggacg cacgccgggc ctcggcacgg tgctggtcgg cgacgacccc
     5881 ggctcgcagg cctatgtgcg cggcaagcac gccgactgcg cgaaggtcgg gatcaactcg
     5941 atccgtcgcg acctgcccgc cgacatcagc caggccgagc tcgaccagac catcgacgag
     6001 ctgaacgcca accccgagtg caccggctac atcgtgcagt tgccgctgcc caagcacctc
     6061 aacgagaacg ccgcactgga gcgtctggag cccggcaagg acgccgacgg cctgcatccc
     6121 atgaacctgg gccggctggt gctcaacgag cccgcgccac tgccgtgcac gccgcggggc
     6181 atagtgcacc tgctgcggcg gtacgaggtg gagatcgcgg gcgcccaggt cgtggtgatc
     6241 ggcagaggtg tgacggtcgg caggccgctc gggctgctgc tcacgaggcg atccgagaat
     6301 gccacagtga cgttgtgtca caccgcgacc cggcatcttc cgcaactgac ccgggaggcc
     6361 gacatcatca tcgccgcggc aggcgtgccg cacatggtga cggcggagat ggtgcgaccg
     6421 ggggccgcgg tcgtcgatgt cggcgtcagc cgcgacgaga acggcaagct ggtcggtgac
     6481 gtggcgcccg atgtgtgg
//
LOCUS       DASYLX010000088         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_443440,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000088 DASYLX010000000
VERSION     DASYLX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443440"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VGP27_02145"
     CDS             <1..602
                     /locus_tag="VGP27_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV7850149.1"
                     /translation="STFDRPPSDHTQAAELAIERAKRLVEQGKDVIVLLDSITRLGRA
                     YNNASPASGRILSGGVDSTALYPPKRFLGAARNIEFGGSLTIIATAMVETGSTGDTVI
                     FEEFKGTGNAELKLDRKISERRVFPAVDVNPSGTRKDELLLAPDEFGIVHKLRRVLSG
                     LDSHQAIDLLMSQLRKTKNNYEFLMQVSKTAPGMADAND"
     gene            complement(621..1232)
                     /locus_tag="VGP27_02150"
     CDS             complement(621..1232)
                     /locus_tag="VGP27_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850150.1"
                     /translation="MTSSTAAKSVRDRLIDAAEACLRAKGIRATTVSEVAEVAQVSRG
                     WLYRHFPDKVSLLGAAIVRLNEVYWSEAHAMLEPLESLDRRIAAGVVHGRTAYDDPGA
                     LLMRLRMAEPEEFAACAGAGVQGLVPDLADFWQRYLVVARDNGEIHTDVDIAEASEWV
                     ARVIISLVTVPGDILDPSDADQVRAHVRRYVMPGLKADPAQSD"
     gene            1378..>2000
                     /locus_tag="VGP27_02155"
     CDS             1378..>2000
                     /locus_tag="VGP27_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850151.1"
                     /translation="MADTLQQLFRERSEQDTVAVKYGDRTWTWREHVAEARAQAAALI
                     DIADPNRPLHVGVLLANGPDMLTAMAAGALGGYVLCCINNTRRGEGLVRDIALVDCQL
                     LITDAAHRHLLDGLDLPGVRVLDTSTEEWTALTAGPKDLTPHREVGPDDTFVMIFTSG
                     TSGDPKAVQVQHMMPIFAGSSLAQRYSVDASDVCYVSMPLFHSNSIYA"
BASE COUNT          343 a          702 c          628 g          327 t
ORIGIN      
        1 cctccacctt cgaccgtccg ccgtccgacc acacccaggc cgccgaactg gcgatcgagc
       61 gggccaagcg cctggtcgag cagggcaagg acgtcatcgt gctgctcgac tcgatcaccc
      121 gcctcggccg tgcgtacaac aacgcgtcgc cggcgtcggg ccgcatcctg tccggtggtg
      181 tcgactcgac cgcgctgtat ccgcccaagc ggttcctcgg tgccgcccgc aacatcgagt
      241 tcggtggctc gctgacgatc atcgccacgg cgatggtgga gaccggctca accggtgaca
      301 cggtgatctt cgaggagttc aagggcaccg gcaacgccga gctcaagctc gaccgtaaga
      361 tctccgagcg ccgcgtcttc ccggccgtcg acgtcaaccc ctccggcacc cgtaaggacg
      421 agctgctgct cgcgcccgac gagttcggaa tcgtgcacaa gctgcgccgc gtgctgtcgg
      481 gtctggactc ccaccaggca atcgatctgc tgatgagcca gctgcgcaag accaagaaca
      541 actacgagtt cctgatgcag gtctccaaga ccgcgccggg gatggcggac gccaacgact
      601 gaccccggcc ggtgcggggc ttagtccgac tgagccgggt cggctttcaa gcccggcatc
      661 acgtagcgcc gcacgtgggc gcgcacctga tcggcgtcgc tcggatcgag gatgtcgccg
      721 ggcacggtga ccagcgagat gatcacgcgg gccacccatt ccgacgcttc ggcgatgtcg
      781 acgtcggtat ggatctcgcc gttgtctcgc gcgacgacca ggtagcgctg ccagaagtcg
      841 gccaaatcgg gcaccagccc ctgaacgccg gcgccggcgc atgcggcgaa ctcttcgggt
      901 tccgccatcc gcagccgcat cagcagcgcg ccagggtcgt cgtaggcggt gcgaccgtgt
      961 accactcccg cggcgattcg ccggtcaaga ctttcgaggg gttccagcat cgcgtgcgcc
     1021 tccgaccagt acacctcgtt gagccggacg atcgccgcac cgagcagcga taccttgtcc
     1081 ggaaagtgcc ggtacagcca gcctcgcgac acctgcgcca cctcggcgac ctcggacacg
     1141 gtggtagcgc ggattccctt ggcgcgcaaa cacgcctcgg ccgcgtcgat caaccgatcg
     1201 cggacactct tggcggccgt gctgctcgtc actcggtcct ctcctcgcaa tccgtcgccc
     1261 caacaggcgc aaccagagca ttctgtcaac tggcaggcgg atactaccgg ccttgccggg
     1321 agatggtaga cagattcgta aatctgttca cttagcgcga cgggatttga ggcgggcatg
     1381 gcggacacac tccagcagtt gtttcgtgag cggtccgagc aggacaccgt cgcggtgaaa
     1441 tacggcgacc gcacgtggac ttggcgcgag cacgtcgccg aggcacgcgc ccaagcggcc
     1501 gcgctcatcg atatcgccga tcccaaccgg ccgctgcatg tcggcgtcct gctggccaac
     1561 gggccggaca tgttgaccgc gatggcggcc ggcgcactcg gcggctacgt cctgtgctgc
     1621 atcaacaaca cccgccgcgg cgagggcctg gtccgcgaca ttgccctggt ggactgtcag
     1681 ctgttgatca ccgacgccgc acaccgccat ctgctggacg ggctcgacct gcccggcgtg
     1741 cgggtgcttg acacctccac cgaggaatgg acggcgctca ccgccgggcc gaaggatctg
     1801 acgccgcacc gcgaggtcgg cccggacgac accttcgtga tgatcttcac ctcgggcacc
     1861 agcggcgacc cgaaggccgt gcaggtgcag cacatgatgc cgatcttcgc gggaagctca
     1921 ctggcgcaac gctattcggt cgatgcatcc gacgtctgct atgtgtcgat gccgctgttc
     1981 cactccaatt ccatctacgc
//
LOCUS       DASYLX010000089         4945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_882410,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000089 DASYLX010000000
VERSION     DASYLX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882410"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGP27_02160"
     CDS             complement(<1..223)
                     /locus_tag="VGP27_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV7850152.1"
                     /translation="MPETGPNILVIWGDDIGISNLSCYSRGMMGYFTPNIDRIAEEGL
                     LFTDSYGEQSCTAGRASFITGQSVYRTGMS"
     gene            complement(226..525)
                     /locus_tag="VGP27_02165"
     CDS             complement(226..525)
                     /locus_tag="VGP27_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV7850153.1"
                     /translation="MPEGRPNILVTWGDDIGISNLSCYSDGLMGYHTPNIDRIANGGM
                     RFTDSYGEQSCTAGRVTAMATMFLETFKEFPPMHEPASFSVDQAVEKLQKFLAKD"
     gene            747..1205
                     /locus_tag="VGP27_02170"
     CDS             747..1205
                     /locus_tag="VGP27_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HEV7850154.1"
                     /translation="MVQIASENATALRLALCSRSTLPGAIDGAWWPQTSDLRTELPDL
                     VAVLGMSIGPVRRVVYDPSVWPNAPSRIIRGTVLTRVDPYTMVASDTIYLMGTHARSA
                     VLFVVPSAIPVAAVRCVLRAVTDATGSVSVAVLRHLVDRFVAETAPSTTY"
     gene            1285..1563
                     /locus_tag="VGP27_02175"
     CDS             1285..1563
                     /locus_tag="VGP27_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dsRBD fold-containing protein"
                     /protein_id="HEV7850155.1"
                     /translation="MYDKSLDDVWRLDIEFNEDETHTHATIRAHLGDGEVLTAIGDAF
                     RNPRDVSQPMIGEEIAVARALISLGTDLLHRAGARIEAVTRHPVHLVG"
     gene            complement(1579..2691)
                     /locus_tag="VGP27_02180"
     CDS             complement(1579..2691)
                     /locus_tag="VGP27_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7850156.1"
                     /translation="MGDATLTTDLGRVLVTGGSGFVGANLVAELLERGHHVRSFDRAP
                     SPLPAHPRLQVVQGDICDKDTVAAAVDGIDTVLHTAAIIDLMGGSSVTDEYRQRSFAV
                     NVGGTETLVMAGQEAGVKRFVYTASNSVVMGGKRISGGDETLPYTERFNDLYTETKVV
                     AERFVLSQNGVRGMSTCSIRPSGIWGRGDQTMFRKLFESVLAGHVKVLVGGKHAKLDN
                     SYVHNLIHGFIVAAEHLVPGGTAPGQAYFINDGEPINMFEFSRPVVEACGQRWPRLRI
                     SGAFVRWVMTIWQWLHFRVGLPKPPFEPLAVERLYLDNYFSIAKAKRDLGYRPLYTTD
                     EAMRDCLPYYLGLFEQMKAEAQQAPVKVAPAGRPSG"
     gene            complement(2755..2967)
                     /locus_tag="VGP27_02185"
     CDS             complement(2755..2967)
                     /locus_tag="VGP27_02185"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV7850157.1"
                     /translation="MKPISELGYEEARDELVEVVRRLEQGGLDLDASLKLWERGEELA
                     KRCDEHLAGARRRIEDALTAGDDDKS"
     gene            complement(2964..4199)
                     /gene="xseA"
                     /locus_tag="VGP27_02190"
     CDS             complement(2964..4199)
                     /gene="xseA"
                     /locus_tag="VGP27_02190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV7850158.1"
                     /translation="MTADERGQSADNPFPVRTVAMMVKDWIEKLGTVWVEGEVAQLSM
                     RPNTNTAFITLRDPAADMSLSVTCPRDLVMNAPVKLSEGTRVIMLGRPNFFVGRGSFS
                     LRVNQIRAVGVGELLARIDRLRKLLAAEGLFDPRLKRPIPFLPSTIGLITGRASSAER
                     DVIAVATDRWPAVSFAVRNTAVQGTSTVPQIVEALRELDADSAVDVIVIARGGGSVED
                     LLPFSDETLCRAISACTTPVVSAIGHEPDNPLCDLVADLRAATPTDAAKRIVPDTAAE
                     LALLLELRGRSARALRNWVHREEHVIGQLRSRPVLAQPLAAQSSRADEVDRCRTAARR
                     DVRRLVTAESERVGHLAARLTTLGPAATLARGYAVVQAVTAAGESAVLHTVDDAPAGT
                     RLRVRVSDGAVMAVSEGSQ"
     gene            complement(4222..4779)
                     /locus_tag="VGP27_02195"
     CDS             complement(4222..4779)
                     /locus_tag="VGP27_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HEV7850159.1"
                     /translation="MATAPYGVRLLVGAAVTAIEETLKLPQTILTYPMTIASQIAHLV
                     MKMQQDVADLVNKGDETLEGLFPPKDEQPEWATFDEDVTDEAPAPSAPDGERLTEGRF
                     ALFTGGEPETEAEVDDPAPVAADLPEVVTRLDYETLTLAQLRARLSTLRVADLEALLA
                     YEESTKSRAPFQTLLANRITRATAK"
     gene            4834..>4945
                     /locus_tag="VGP27_02200"
     CDS             4834..>4945
                     /locus_tag="VGP27_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV7850160.1"
                     /translation="MPPTINMGIPGASISASSGVAGKRVLLAEPRGYCAGV"
BASE COUNT          810 a         1590 c         1594 g          951 t
ORIGIN      
        1 tgctcatgcc ggttcggtac acgctctggc cggtgatgaa cgacgcacgg cctgcggtgc
       61 agctctgctc gccgtaggag tcggtgaaaa gcaatccttc ctcggcgatc cggtcgatgt
      121 tgggcgtgaa gtaacccatc attccgcggc tgtaacagct caggttcgag ataccgatgt
      181 cgtcgcccca gatgacgaga atgttcggcc ccgtttcggg catggctaat ccttcgctag
      241 gaatttttgg agcttttcga cggcctggtc gacgctgaag ctggccggct cgtgcattgg
      301 cggaaactcc ttgaacgttt ccaagaacat cgtcgccatc gcggtcacgc ggcccgctgt
      361 gcagctttgc tcaccgtagg agtcggtgaa tcgcatacct ccgttggcaa tgcggtcgat
      421 gttcggggtg tggtagccca tcaaaccgtc gctgtaacaa ctcaggttgc tgatcccgat
      481 gtcgtcgccc caggtgacca ggatgttcgg cctgccttca ggcatgcgac gcccctttgc
      541 ctacgccccg accgaatccg atgtgcgact tgaccttatt cgggttccgg tcagtattgg
      601 tccgcttcag caggctgcca gaaaaaattt acggagacgt cgaaattccg gtgtgggtta
      661 tggtcagtga atcgtaggtt cgtcgcgtgt ccgacatctc ggctgccgtc tgcgattgct
      721 cgactacctg actggagcag cgcccgatgg tgcagatcgc ttccgagaat gcgactgcgc
      781 tccggttggc gttgtgtagc cgttcgacat tgccgggcgc gatcgatggc gcttggtggc
      841 cgcagacttc cgatctacgg accgaactgc ctgacctcgt cgccgttctg gggatgtcaa
      901 tcggtccggt tcgtcgggtg gtctacgacc cgagcgtgtg gccgaatgcg ccgtcgcgaa
      961 tcattcgcgg caccgtactg accagagtcg atccgtacac gatggttgcc agcgacacga
     1021 tttacctgat gggcacgcat gcccggtctg cggtgctctt cgtcgtgccc tccgcaatcc
     1081 ccgtcgctgc tgtccgttgc gttctgcgtg ccgtgaccga cgcgaccggg tcagtcagtg
     1141 tggccgtgct gcgacacctg gtcgaccggt tcgtcgcgga aaccgccccg tcgaccacgt
     1201 actagggccg gattgcagca aacgcgccat ttcccggtgc gcagccgagc agcggagcac
     1261 tctataggcg ggaaggtggt gtatatgtac gacaagtctc tggatgacgt ttggcgcctc
     1321 gacatcgagt tcaacgagga cgagacgcat acgcatgcga cgatccgcgc tcatctcggt
     1381 gacggtgagg tcttgaccgc gatcggtgat gcgttccgta accccaggga cgtcagtcag
     1441 ccgatgatcg gcgaggagat cgcggtcgcc agggcgctga tctcactggg cacggatctg
     1501 ctccaccgcg ccggcgccag gatcgaggcg gtgacgcgcc acccggtcca cttggtgggt
     1561 tgacgctttt ccggatcgtc agcccgacgg acggcccgcc ggggccacct tgaccggcgc
     1621 ttgctgcgcc tcggccttca tctgctcgaa gagtccgagg taatacggca ggcagtcgcg
     1681 catcgcctcg tcggtggtgt acagcggccg atagccgaga tcgcgcttcg ccttggcgat
     1741 cgagaagtag ttgtcgaggt acaggcgctc gacagcgagc ggctcgaacg gcggcttggg
     1801 cagcccaacg cggaagtgca gccactgcca gatcgtcatg acccagcgca cgaacgcgcc
     1861 cgagatgcgg agcctgggcc agcgttgtcc gcacgcctcg acgacggggc gggagaactc
     1921 gaacatgttg atcggctcac cgtcgttgat gaagtacgcc tgcccgggag ctgtcccgcc
     1981 gggcaccagg tgctctgcgg ccacgatgaa accgtgtatc aggttgtgca cgtaggagtt
     2041 gtccagcttg gcgtgcttac cgccgaccag caccttgacg tggccggcga gcacgctctc
     2101 gaacaacttg cggaacatgg tctggtcgcc acggccccag ataccgctgg gccggatcga
     2161 acaggtcgac attccccgaa cgccgttctg cgacaataca aatcgctcgg cgaccacttt
     2221 ggtctcggtg tagaggtcgt tgaatcgttc ggtgtagggc agcgtttcgt ctccgccgga
     2281 gattcgctta ccgcccatca ccacgctgtt ggaagcggtg tagacgaacc gcttcacccc
     2341 ggcctcctga ccggccatca ccagggtctc ggtgccgccg acgttgaccg cgaagctgcg
     2401 ctggcggtac tcgtcggtga ccgacgagcc gcccatcagg tcgataatcg ctgcggtatg
     2461 gagcacggtg tcgatgccgt cgacggccgc tgcaacggtg tccttgtcgc agatgtcgcc
     2521 ctggacgacc tgaagccggg ggtgagcggg cagcggagag ggcgcgcggt cgaacgagcg
     2581 cacatgatgt ccgcgctcga gcaactctgc caccagattc gcccctacga agccggagcc
     2641 tccggtgacc aggacacggc cgagatcagt cgtaagcgtt gcatcaccca tgcgggcgag
     2701 cctaactgaa acgtgttcca gtttcgagca cctttagcga atttgtttga ttgatcaact
     2761 tttgtcgtca tcgccggcgg tcaacgcgtc ctcgattcgc ctgcgggcgc ccgcgaggtg
     2821 ttcatcgcat cgtttggcca gctcttcgcc tctttcccag agcttgaggg aggcatcaag
     2881 atcgagtccg ccctgctcca gccggcggac gacttcgacc agctcgtcac gggcttcttc
     2941 gtaacccaat tcactaatag gcttcattga cttccctcgc tgacggccat taccgccccg
     3001 tcggacaccc gcacccgcag ccgcgttccc gcaggcgcat cgtccacggt atgtaggaca
     3061 gccgactcgc ccgcggccgt taccgcctgc accaccgcat agccgcgggc cagggtcgcg
     3121 gcgggcccga gtgtcgtcag ccgggccgcc aggtggccga ctcgctccga ttcggcggtg
     3181 accaggcgcc tgacgtcacg acgggcggcg gttctgcagc ggtcgacctc gtcggcacgt
     3241 gagctctgcg cggccagcgg ctgcgccagc accggtctgc tgcgcagctg gccgatgacg
     3301 tgctcctcgc ggtgcaccca gttgcgcagt gcccgcgcgc tgcgcccgcg cagctcgagg
     3361 agcagcgcca gttcggcggc ggtgtcggga acgatgcgtt tggcggcatc ggtcggggtg
     3421 gcggcccgca gatcggcgac cagatcgcac agcgggttgt cgggctcgtg cccgatggcg
     3481 ctgacgaccg gcgtggtgca tgccgagatc gcccggcaca gcgtctcgtc ggagaacggc
     3541 aacagatcct cgacgctgcc gccgccgcgt gcgatgacga tgacgtcgac ggcggaatcg
     3601 gcatcgagtt cgcgcaacgc ctccacgatc tgcgggacgg tgctggtgcc ctgcaccgcg
     3661 gtgttgcgaa cggcgaaact tactgccggc cagcgatccg tggccaccgc gatgacgtca
     3721 cgttcagcgg aactcgcccg cccggtgatc aatccgatag tgctgggcag aaagggaatt
     3781 ggccgcttca gccggggatc gaacaggcct tctgcggcca gcagtttgcg cagccggtcg
     3841 atccgggcta gtagctctcc gacgccgacc gcgcgaatct ggttgacccg caacgagaat
     3901 gagccacggc cgacgaagaa gttcggccgt ccgagcatga tcacccgggt tccttcggac
     3961 agcttgacag gcgcattcat cacgaggtcg cgcgggcagg tcaccgacag cgacatgtcg
     4021 gcggccgggt cacgcagtgt gatgaacgcc gtattcgtgt tgggccgcat cgacagttga
     4081 gcgacctcgc cttcgaccca gacggttccg agcttctcga tccaatcctt gaccatcatc
     4141 gcgacggtgc ggacggggaa gggattgtcc gcggattggc cccgctcgtc tgcggtcatt
     4201 ctgccgagcc cgctcgggtg gtcacttggc cgtcgcgcgg gtgatcctgt tggccagcaa
     4261 tgtctggaac ggtgcgcgcg atttggtcga ctcttcgtat gccagcagcg cctcgaggtc
     4321 ggcgacacgc agcgtcgaca gccgcgcccg caactgcgcc agcgtcagcg tctcgtagtc
     4381 cagacgcgtg acgacctccg gcaggtcggc cgctaccggc gccgggtcgt ccacctccgc
     4441 ctccgtctcc ggctcaccgc cggtgaacag agcgaacctg ccctcggtga gtcgttcgcc
     4501 gtcaggggcc gacggtgccg gcgcctcgtc ggtcacgtcc tcgtcgaacg tcgcccactc
     4561 gggttgctca tccttcggcg ggaacagacc ctccagcgtc tcgtcgccct tgttgaccag
     4621 atcggccacg tcctgctgca tcttcatcac caggtgcgcg atctgactcg cgatggtcat
     4681 cgggtaggtg agaatggtct gcggaagctt gagtgtttcc tcgatggcgg ttaccgccgc
     4741 tcctaccagc agacggacgc catacggtgc agtagccatg ggccccagcc tacggctggc
     4801 ctctcggcag tgcgggtcag taccctggga tccatgccac cgactatcaa catggggatt
     4861 cccggcgcct ccatctcggc gtccagcggt gtggcaggta aaagggttct gctggccgaa
     4921 ccccgcggat attgcgcggg tgtgg
//
LOCUS       DASYLX010000090         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3296045,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000090 DASYLX010000000
VERSION     DASYLX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296045"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGP27_02205"
     CDS             complement(<1..389)
                     /locus_tag="VGP27_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7850161.1"
                     /translation="MTRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPTDHLEAVTDEE
                     GVADLAALSDGLRAEREQGITIDVAYRFFSTDARSYILADTPGHERYTRNMFTGASNA
                     HVAVLLVDARAGVLRQTRRHARIAKLLG"
     gene            complement(389..1315)
                     /gene="cysD"
                     /locus_tag="VGP27_02210"
     CDS             complement(389..1315)
                     /gene="cysD"
                     /locus_tag="VGP27_02210"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEV7850162.1"
                     /translation="MTTTTSTYDTTHVDELRLLEAEAVHIIREVVAELERPVLLFSAG
                     KDSIVLLRLAEKAFRPSPLPFPVMHVDTGHNFDEVIEFRDRRVAGEGHRLIVASVQES
                     IDKGRVPDPGPGASRNRQQTRTLLDALEEGGFDAAFGGARRDEERARAKERILSFRDE
                     FGQWDPRSQRPEPWSLYNGRIKNGEQVRVFPLSNWTELDVWRYIELENLELPPIYFAH
                     EREVFERDGILLAVSEYAGPTDGETASVEWVRYRTVGDLTITGAVRSTATDIERVIEE
                     ISAATVSERGETRADDRTSAAAMEDRKREGYF"
     gene            complement(1312..2109)
                     /locus_tag="VGP27_02215"
     CDS             complement(1312..2109)
                     /locus_tag="VGP27_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEV7850163.1"
                     /translation="MAASAYLVLASQRSGSTLLVESLRATGVAGEPGEFFQYLPSTSQ
                     SPQPRQWFEGVADESILRLLDPLDEGKPDLAPPEIWRDYIRSAGRTPNGIWGGKLMWN
                     QASLLLHRADGLPDRSGDGLLSAIRDVIGTDPVLVYVYRPDVVSQAVSFWRAVQTRVW
                     RGRPDPMRDSRAEYHAGAIAHVITMLREQEKGWRGWFDEEGVSPMEISYPVLWRNLTS
                     IVGTVLDALGQDPRLAPAPVLERQADQRSDEWVDRYRADAEKEGLPT"
BASE COUNT          348 a          699 c          719 g          384 t
ORIGIN      
        1 cccaacagtt ttgcgatccg ggcatgcctg cgggtttggc gcaggacgcc cgcgcgggcg
       61 tcgaccagca gcaccgcgac atgtgcattg gacgcgcccg tgaacatgtt gcgggtgtag
      121 cgttcgtgtc ccggcgtatc ggcaaggatg tagctgcggg catcggtcga gaagaagcgg
      181 taggcgacgt cgatcgtgat gccctgctcg cgctcggcgc gcaggccgtc ggacagggcg
      241 gccagatcgg cgacgccctc ctcgtcggtg acggcttcca gatggtcggt cggaaggctg
      301 tcggtatcgt gcagcaagcg gccgatcaat gtgctcttac cgtcgtcgac ggatcctgcg
      361 gtggtgattc tcagcagttg gcgtgtcatc agaagtagcc ctcgcgcttg cgatcctcca
      421 tggcggccgc cgacgtccgg tcgtcggcac gggtctcgcc gcgttcggaa acggtggccg
      481 cggagatctc ttcgatcacg cgctcgatgt cggtggccgt cgatcgcacc gcgccggtga
      541 tggtcaggtc gccgactgtc cggtagcgca cccattcgac cgaagcggtt tcaccgtctg
      601 tgggcccggc gtactcggac accgcgagca gaatgccgtc gcgctcgaac acctcgcgct
      661 catgggcaaa gtagatcggc ggtagctcga gattctccag ctcgatgtag cgccagacat
      721 ccagctcggt ccagttgctg agcgggaaga ccctgacctg ctcgccattt ttgatgcggc
      781 cgttgtacaa cgaccagggc tccgggcgtt gcgagcgggg gtcccactgg ccgaactcgt
      841 cgcggaagct gaggatgcgt tccttggctc gggcgcgttc ctcgtcgcgg cgggcgccgc
      901 cgaacgcggc gtcgaaccca ccctcctcca gcgcgtctag cagcgtgcgg gtctgctgtc
      961 ggttacggga ggcgccggga cccggatcgg gaacgcgtcc cttgtcgatc gactcctgca
     1021 ccgacgcgac gatcaacctg tgtccttctc cggcgaccct gcgatcgcgg aactcgatca
     1081 cctcatcgaa attgtggccg gtgtcgacat gcatcaccgg aaagggcagt ggcgacggcc
     1141 gaaaggcctt ctccgccaac cgcagaagca cgatggagtc cttgcctgcg gagaacagca
     1201 gcaccggccg ctccagctcg gcgacaacct cgcggatgat gtgcaccgcc tccgcttcaa
     1261 gcaggcgcag ttcatcgaca tgggtggtgt cataggtgga ggtggtcgtg gtcatgtcgg
     1321 cagcccttcc ttctcggcgt cggcacggta gcggtctacc cattcgtcgg acctttgatc
     1381 ggcctgacgt tcgaggaccg gcgcgggcgc cagtcgcgga tcctgtccca gcgcgtcgag
     1441 cacggtcccg acgatcgagg tcaagttgcg ccacagcacg ggataggaga tctccatcgg
     1501 ggagacgccc tcttcgtcga accacccccg ccaacccttc tcctgctcgc gcagcatggt
     1561 gatgacgtgg gcgatggcgc ccgcgtggta ctcggcgcgt gagtccctca tcgggtcggg
     1621 cctaccgcgc cagacccgcg tctgcaccgc ccgccagaac gaaaccgctt gcgacacaac
     1681 gtcaggacgg tagacataga cgagcaccgg atcggtgccg atgacatctc ggatcgcgga
     1741 gagcaggccg tcgccggagc ggtcgggcaa cccatccgcg cggtgaagca gaagcgaggc
     1801 ctggttccac atcagcttgc cgccccagat gccgttgggg gttcgtcccg ccgaccgaat
     1861 gtagtcacgc catatctcgg gcggcgcaag gtccggcttg ccttcgtcga gcgggtcgag
     1921 aaggcgcagg atcgattcgt ccgcaacccc ttcgaaccac tgccttggct gcggcgactg
     1981 gctggtgctc ggcaggtact ggaagaactc acccggctcg ccggcaactc cggtggcccg
     2041 cagggattcg acgagcaggg tgctgccact gcgttgcgat gcgagtacga ggtacgcgga
     2101 ggcagccatc ggatccccta actgtgttcg tcttcgatgc cgcggtcggc
//
LOCUS       DASYLX010000091         6430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2308773,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000091 DASYLX010000000
VERSION     DASYLX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308773"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGP27_02220"
     CDS             <1..382
                     /locus_tag="VGP27_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV7850164.1"
                     /translation="GRVPFLDHELVELAATCPPDLKTAQGGKGVLKEAARRVIPSEVI
                     DRPKGYFPVPALTHLEGPYLDMVRDALYAPVAKERGLFQPDAVDALLADPNGRLTPLR
                     GNELWQIALLELWLQKHGISGPAA"
     gene            379..2139
                     /gene="ngg"
                     /locus_tag="VGP27_02225"
     CDS             379..2139
                     /gene="ngg"
                     /locus_tag="VGP27_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HEV7850165.1"
                     /translation="MSGTEVHSEAITLALHDASPPDLVDAMADDIELELGWGRLIFGQ
                     TFADADKLAEVLRGETHGRRDICMYAREPHVLVSKAPAELFIDPSHTYRLRFSDDDEP
                     SPAPSGFTVRALQDEAEADAIDNVYVRCGMVPAPTAVIWDNHRHHEAVDYLIAVRDDD
                     GSVVGTVTGVDHKLLFSDPEDGSSLWTLAVDPTTSLPGIGAALTRALADVYRDRGRAY
                     MDLSVTHDNAAAIALYEKLGFQRIPVMAVKRKNAINEPLFTHPPETVDDLNPYARIIA
                     DEAMRRGIWVEVLDAGAGEMRLSHGGRTVVTRESLSEYTSAVAMSRCDDKRQTRRIVG
                     EAGIMVPKGRLATFDEGDHEFLAEVGDVVVKPTRGEQGKGITVGVDGPDELNSALARA
                     REQHPEVLIEQRAKGEDLRLVVIDGRVVAAAIRRPAEIVGTGHHTVRDLIETQSRRRA
                     AATGGESRIPVDAVTEATVAEAGWSFDDVLPEGERLRVRRTANLHQGGTIHDVTAEVH
                     PELCRVAVTAAEAIGIPVTGIDLLVPDVTADGYVFIEANERPGLANHEPQPTAAAFVD
                     YLFPTTPGLPQAWTPTEAPD"
     gene            complement(2146..2709)
                     /locus_tag="VGP27_02230"
     CDS             complement(2146..2709)
                     /locus_tag="VGP27_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850166.1"
                     /translation="MPSTMVTVDGFPVPVDVAGPDKGSVVVLLGAAQQAASAYDAVCQ
                     RLHTASLRTIVVGPDPRLTAKSVVGIMDMLDVQWGLLVGDRIGGELAWELAATRLDRF
                     IGLVVIDRGHPRVADQAGAIRDEHCPPVEMNTTALVSSPAARAVARASQRYVYGDFRL
                     VELLGRRNAQESTAQLAAEIVLRTSTW"
     gene            complement(2772..3443)
                     /locus_tag="VGP27_02235"
     CDS             complement(2772..3443)
                     /locus_tag="VGP27_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850167.1"
                     /translation="MIGARVAAAALAVLVVFAPTGCGTMISGTATWPGAKLDRTVLTG
                     ADFPAGVQYDRIVEKPGHADGEGGPPAMLSKPEGCSDALTRVIAATAQRGAGSASKYS
                     VAYDGARIVMTVLTWNLDLDKLAATAERCAQYQTFFDPASEGIPITTTRLQTARPDAL
                     VYEQTMNLSGAKSSVFFAFENVDTMAAFGMAFPTPNPTIVVKGSLPQTFLDVSSKQSE
                     RLRNG"
     gene            complement(3454..4941)
                     /locus_tag="VGP27_02240"
     CDS             complement(3454..4941)
                     /locus_tag="VGP27_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEV7850168.1"
                     /translation="MSGALLVAGTTSDAGKSMVVAGLCRLLARKGITVAPFKAQNMSN
                     NSAVTVEGGEIGRAQAMQARAAGLAPSIRFNPILLKPGSDRTSQLVVKGRVAGTVAAA
                     DYIEHRDRLAEVVADELASLRAEFDAVICEGAGSPAEINLRARDLVNMGLARAADLPV
                     VVVGDIDRGGLLAHLFGTVAVLAPDDQALIAGFVVNKFRGDPALLAPGLTALEHLTGR
                     PTYGVIPFDDGFWLDTEDSVSVQAHRLVGRPQPPVGEQWLRVAAIRLPRISNSTDVVA
                     LACEPGVLGRWVTDPADLPDADVVVIPGSKATVADLNWLRDRGLADGVTAHARSGRAV
                     LGICGGFQMLCRRIDDPVESGAGSVDGLGLLDADILFAADKTLRRHQTPLHGYEIHHG
                     QVSRAADDDWLGVGVRCAEVYGTHWHGLFDNDQVRRRWLAGVAAAAARHGFTVADDVD
                     VSARRDAQLDLMADLLAAHADVDALLGLLDTGPPERPAIVSTLRR"
     gene            complement(4946..5803)
                     /gene="map"
                     /locus_tag="VGP27_02245"
     CDS             complement(4946..5803)
                     /gene="map"
                     /locus_tag="VGP27_02245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV7850169.1"
                     /translation="MPVRTLLRPGAVSPTLPVPNSIARPEYVWKATAREGTEPWVQTP
                     EVIDKMRVAGRIAAGALAEAGKAVAPGITTDELDRIAHEYMVDHGAYPSTLGYKGYPK
                     SCCTSLNEVICHGIPDSTVVEDGDIVNIDVTAYIDGVHGDTNATFLAGDVSEEHRLLV
                     ERTHEATMRAIKAVRPGRALSVVGRVIESYANRFGYNVVRDFTGHGIGTTFHNGLVVL
                     HYDQPAVDTILEPGMTFTIEPMINLGALDYEIWDDDWTVVTTDRKWTAQFEHTLVVTD
                     DGAEILTQL"
     gene            complement(5820..6404)
                     /locus_tag="VGP27_02250"
     CDS             complement(5820..6404)
                     /locus_tag="VGP27_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEV7850170.1"
                     /translation="MTGINEQSAAMRISDADRNGTLRRLHNAVALGLIDIDEFEERSV
                     LVSQARLQSDLDTLVGDLPGPGAIVTSATDRVELRGVMGSLKRQGEWTVPTRLALHRR
                     MGSVQLDLTRARFAGPMVIIELDMKFGGLDLRLPDGASASIDDVEVVVGSADDHRRDA
                     PADGTPHVILTGKVVCGSVDIRGPRRSWFGRDRH"
BASE COUNT         1039 a         2187 c         2202 g         1002 t
ORIGIN      
        1 gggacgagtg ccgttcctcg atcacgaact cgtcgagttg gccgctacct gcccgccgga
       61 tctcaagacc gcgcagggcg gcaagggtgt cctcaaggag gccgcccgca gggtgatccc
      121 gtcggaggtc atcgaccgcc ccaagggcta cttcccggtg cccgcactga ctcatctcga
      181 gggcccgtac ctcgacatgg tgcgcgacgc gctgtacgca ccggtcgcca aggagcgcgg
      241 cctgttccaa cccgacgccg tggacgcgtt gcttgccgat cccaacggcc gattgactcc
      301 gttgcgcggc aacgagttgt ggcagatcgc gttgctcgag ctgtggctgc agaagcacgg
      361 tatctcgggg cccgcggcat gagcggtacc gaggttcatt cggaggcgat caccctcgcg
      421 ctgcacgacg cgtccccccc agacctcgtc gacgcgatgg ccgacgacat cgagcttgaa
      481 ctcggctggg gccgtctgat tttcggccag acattcgccg acgccgacaa gctggccgag
      541 gtgcttcgag gagagacaca cggcagacgc gacatctgca tgtacgcccg cgagccacat
      601 gtcctcgttt ccaaggcgcc cgccgagttg ttcatcgacc cgagccatac ctaccggctg
      661 cggttctccg acgacgacga gccttctccg gcgccgtcgg gtttcaccgt gcgcgcgttg
      721 caggacgagg ccgaggccga cgcgatcgac aacgtctacg tccgctgcgg gatggtgccc
      781 gcacccacgg ccgtcatctg ggacaaccac cgccaccacg aagcagtcga ctacctcatc
      841 gcggtccgtg acgacgacgg ctcggtggtg ggcacggtga cgggtgtcga ccacaagctg
      901 ctgttctccg accctgagga cggctcgagc ctgtggacgc tggccgtcga cccgacgacc
      961 agcctgccgg gcatcggcgc tgcattgacc cgcgcgctcg ccgacgtcta ccgcgaccgg
     1021 ggccgggcgt acatggatct ctcggtcacg catgacaacg cggccgccat cgcgctctac
     1081 gaaaaactgg ggttccagcg cattccggtg atggccgtca agcgcaagaa cgcgatcaac
     1141 gaaccgctct tcacccatcc gccggagacg gtcgacgacc tgaaccccta cgcgcgcatc
     1201 atcgccgacg aagccatgcg ccgcggcatc tgggtcgagg tgttggacgc aggcgcagga
     1261 gagatgcggc tgtcgcacgg tggtcgcacc gtcgtgaccc gagaatcgtt gtccgagtac
     1321 acatcagcag tggccatgag ccgttgtgat gacaaacggc agacgcgccg catcgtcggc
     1381 gaagccggga tcatggtccc gaagggccgt ctcgccacct tcgacgaggg cgaccacgaa
     1441 ttcctcgccg aggtcggcga cgtggtggtc aaaccgacca ggggcgagca gggcaagggc
     1501 atcaccgtcg gcgtcgacgg accggatgaa ctcaacagcg ccctggcccg ggcccgcgag
     1561 caacatcccg aggtgctcat cgagcagcgc gcgaagggcg aggatcttcg gcttgtggtg
     1621 atcgacggca gggtcgtcgc ggccgcgatc cgcaggcccg ccgagatcgt cggcaccggc
     1681 caccacaccg tgcgagatct gatcgagacg cagagcaggc gccgggcggc ggccaccggc
     1741 ggcgagtcac gcatccccgt cgacgccgtc accgaggcga cggtggccga ggccggatgg
     1801 tccttcgacg acgttctgcc cgagggagag cggctgcgcg tgcgacgcac cgcgaacctg
     1861 catcagggcg gcacgattca tgacgtgacg gccgaggtgc acccggagct gtgccgggtg
     1921 gccgtcacgg ccgccgaggc catcggcatc cccgtcaccg ggatcgacct gctggtgccg
     1981 gacgtcacgg ctgacgggta cgtgttcatc gaagccaacg agcggccggg actggccaat
     2041 cacgagcccc agcccacggc cgcggccttc gtcgattacc ttttccccac gacgccgggt
     2101 ttgcctcagg cgtggacgcc gaccgaggca cctgactgag cgggctcacc acgtactggt
     2161 gcgcaacacg atttcggcgg ccagttgcgc ggtggactcc tgggcgttgc gccgcccgag
     2221 cagctcgacc agcctgaagt cgccgtagac gtagcgctgg ctggcccggg ccaccgcccg
     2281 cgccgcaggt gagctgacca gcgcggtggt gttcatttcc accggcgggc agtgctcgtc
     2341 gcggatcgcg cccgcctggt cggcgacgcg tggatgaccg cggtcgatca cgaccagacc
     2401 gatgaagcgg tccaggcggg tagccgccag ctcccatgcc agctcaccgc cgatccggtc
     2461 gccgacgagc agaccccact gcacgtcgag catgtccatg atgccgacga cggatttggc
     2521 ggtcagccgt ggatccggac caaccacgat ggtccgaagc gacgcggtat ggagccgctg
     2581 gcacacggcg tcatacgccg acgcggcctg ctgtgcggcg ccgagcagca ccacgacgga
     2641 ccccttgtcc gggccggcga catcgacggg aacagggaaa ccgtcgacgg taaccatcgt
     2701 cgaaggcatt gggtcaaact acagggcgtc ggggaacggc gggtggtttt tgcgtgggtc
     2761 ccacccagtc atcagccatt acgcagccgc tcggattgtt tgctgctgac atccagaaac
     2821 gtttgcggca gcgagccttt gacgacgatc gtgggattcg gagtgggaaa cgccatgccg
     2881 aacgccgcca tcgtgtcgac gttttcgaac gcgaagaaca cgctgctctt cgccccgctg
     2941 aggttcatcg tctgctcgta caccagcgcg tcgggccgcg cagtctgcag ccgcgtggtg
     3001 gtgatcggaa tgccttccga cgccgggtcg aagaaggtct ggtattgggc acagcgctcg
     3061 gccgtggccg cgagcttgtc cagatccagg ttccacgtca gcaccgtcat cacgatgcgc
     3121 gcgccgtcgt aagcgacgct gtacttcgag gcgctgcccg cgccccgttg cgccgtcgcg
     3181 gcgatgaccc gcgtcagtgc gtcggagcag ccttcgggtt tcgacagcat cgccggcgga
     3241 ccgccttcgc cgtcggcgtg gccgggtttc tcgacaatcc ggtcgtactg cacgcccgcc
     3301 ggaaagtccg cgccggtcag cacggtccga tccagcttgg cgccgggcca ggtcgcggtg
     3361 cccgaaatca tggttccgca gccggtgggc gcgaagacca ccagtactgc cagcgcagcc
     3421 gccgcgacgc gcgccccgat catggggcca aggctaccga cgcagagtgc tgacgatggc
     3481 gggccgctcg ggaggtccgg tatccagcag gccgagcagc gcgtcgacgt cggcgtgggc
     3541 cgccagcagg tcggccatca ggtcgagctg ggcgtcgcgc cgtgccgaga cgtcgacgtc
     3601 gtcggcgacg gtgaagccgt gtcgtgcggc ggccgcggcc accccggcga gccagcgtcg
     3661 ccgcacctgg tcgttgtcga acagcccgtg ccaatgggtg ccgtagacct cggcgcaccg
     3721 cactccgacg ccgagccagt cgtcgtcggc ggctcgcgag acttggccgt ggtggatctc
     3781 gtagccgtgc agcggcgtct ggtggcggcg cagcgtctta tcggccgcga acaggatgtc
     3841 ggcgtcgagc aggccgaggc cgtcgacact gcccgccccg gattccacgg ggtcgtcgat
     3901 gcggcggcac agcatctgga agccaccgca gatgccgagc acagcccgac ccgaccgggc
     3961 gtgcgcggtg acgccgtcgg ccaggccgcg gtcgcgcagc cagttcaggt cggcgacggt
     4021 cgccttgctg cccgggatga ccacgacgtc ggcgtcgggc aggtcggcgg gatcggtcac
     4081 ccaacgcccc agcacgccag gctcgcacgc gagagctacc acgtcggtgg aattggagat
     4141 gcggggcagc cggatggcgg cgacccgcag ccactgctca ccgacaggcg gctgcggccg
     4201 gccgacgagg cggtgggcct gcaccgacac cgagtcctcg gtgtcgagcc agaagccgtc
     4261 atcgaacggg atgacaccgt atgtgggtct gccggtgagg tgttcgagcg ctgtgagtcc
     4321 gggcgccagc agggccggat cgccgcggaa cttgttgacg acgaacccgg cgatgagcgc
     4381 ctgatcgtcc ggcgcgagca ccgcgacggt gccgaacagg tgggccagca ggcccccgcg
     4441 gtcgatgtcg ccgacgacga cgacgggcag atccgccgcc cgcgccagac ccatgttcac
     4501 cagatccctt gcgcgcaggt tgatttcggc gggtgacccg gcaccttcac agatgacagc
     4561 gtcgaattcc gctctcaagg aggctaattc gtcggcgacc acctcggcga ggcggtcgcg
     4621 gtgttcgatg tagtcggctg cggcaacggt gcccgcgacg cgacccttga cgaccaactg
     4681 cgacgtgcgg tcgctgcccg gcttgagcag tatcgggttg aaccggatgc tcggagccag
     4741 tcccgcggcg cgcgcctgca tcgcctgcgc ccgcccgatc tcgccgccct cgacggtgac
     4801 cgccgagttg ttcgacatgt tctgggcctt gaacggcgcc accgtgatgc ccttacgggc
     4861 cagcagccgg cacaagcctg cgacgaccat ggattttccg gcgtccgacg tcgtgccagc
     4921 gaccagtaac gcgccgctca cgagctcaca gctgggtcag gatttcggcg ccgtcgtcgg
     4981 tgaccaccag ggtgtgttcg aactgagcgg tccacttgcg gtccgtggtg acgacggtcc
     5041 agtcgtcgtc ccagatctcg tagtccagcg cgccgaggtt gatcatcggc tcgatggtga
     5101 acgtcatccc cggttccagg atggtgtcga cggcgggctg gtcgtagtgc aacaccacca
     5161 ggccgttgtg gaaggtggtg ccgatgccgt gtccggtgaa atcgcgaacc acgttgtaac
     5221 cgaagcggtt cgcatacgac tcgatgacgc ggccgacgac cgacagagcg cggccgggcc
     5281 gcaccgcctt gatcgctcgc atcgtcgctt cgtgcgtgcg ctccaccagc agccggtgct
     5341 cctcggacac gtcgcccgcg aggaaggtcg cgttggtgtc gccgtggact ccgtcgatgt
     5401 aggcggtgac gtcgatgttg acgatgtcgc cgtcctcgac gacggtcgag tccgggatgc
     5461 cgtggcagat gacctcgttg agcgaggtgc aacaggactt cgggtagccc ttgtagccca
     5521 gcgtcgacgg gtaggcgccg tggtcgacca tgtactcgtg tgcaatgcgg tcgagctcgt
     5581 cggtggtgat ccccggcgcg acggccttac ccgcctcggc gagtgcgccc gccgcgatgc
     5641 ggccggccac ccgcatcttg tcgatcacct cgggggtctg cacccacggt tcggtgcctt
     5701 ccctggccgt cgccttccat acgtactcgg ggcgggcgat cgagttgggc accggcaggg
     5761 tcggcgacac cgcgccgggg cgaagtaggg tgcgaacagg catgacgtca gaatagtcgt
     5821 cagtgtcggt cgcggccgaa ccacgacctg cgcgggccac gaatgtcgac cgaaccgcag
     5881 accaccttgc cggtcaggat gacgtgcggg gtgccatccg cgggcgcgtc cctgcggtga
     5941 tcatcggcgc tgccgacgac gacctcgaca tcgtcgatcg acgcgctcgc cccgtcgggc
     6001 agccgcagat ccagcccacc gaacttcatg tcgagctcga tgatcaccat cggcccggcg
     6061 aaccgcgcac gggtcaggtc gagctggacc gagcccattc gccggtgcag cgccagccgg
     6121 gtcggtaccg tccactcccc ctgacgctta agcgacccca tcacgccgcg cagctcgacc
     6181 ctgtcggtgg cggaggtgac gatcgcgccg ggtcccggca ggtcgccgac gagtgtgtcg
     6241 aggtcggatt gcagccgggc ctgcgacacc agaaccgagc gctcctcgaa ctcgtcgatg
     6301 tcgatcaggc cgagggcgac ggcgttgtgc aggcggcgca gggtgccgtt gcggtcggcg
     6361 tccgatatac gcatcgcggc tgattgctca ttgatcccgg tcatggccac tccaaaatac
     6421 cgccgctgcc
//
LOCUS       DASYLX010000092         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3076143,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000092 DASYLX010000000
VERSION     DASYLX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076143"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..904
                     /locus_tag="VGP27_02255"
     CDS             149..904
                     /locus_tag="VGP27_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV7850171.1"
                     /translation="MPTDAEHAPFLFLSIRGEDAAADDEYQAMMRFGGLDQHGMQRIR
                     LTHRALGAVDLDDWSGIILGGGPYNASDPPDSKSSTQRRVEAELLSLVDRIVAADFPF
                     LGCCYGVGMLGSVIGATVDRSYPEPVAAVTVTLTPAGREDPLFAELPDVFDAFGGHKE
                     AASSLPSAAVCLASSPDCPVQAFRVGTNTYATQFHPELDLQGILTRIDVYKTYGYFAP
                     ESAESLKTAARQRHIEYPPTILRRFVQRYSRPR"
     gene            complement(914..>1735)
                     /locus_tag="VGP27_02260"
     CDS             complement(914..>1735)
                     /locus_tag="VGP27_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEV7850172.1"
                     /translation="SKVAGRPLSQQQVVMLGAGSAGIGVLGMVQREMVAQGLSEAEAA
                     ERIWVVDVIGLLTDDRTDLSPAQRRFAQPAGRVAGWGLSGPPQLADVVHNVDVGILMG
                     LSTAAGAFTESIVRELAAKTERPIIFPLSNPTSHAEAHPAELDQWTDGRALIATGSPF
                     APLQRDGVARPIAQCNNVYIFPAMGLAVTAAQATRVTDDMMRVAAATLGDASPALSDP
                     AAPLLPAWSDVPDIAQDIAHAVGRQAIAEGVAPARSDEQLADRIRQVHWTPEYPS"
BASE COUNT          277 a          593 c          586 g          279 t
ORIGIN      
        1 tcaccagggc cccgatgagc gtcgcgccgg tgccgaagcc ggtccccgga agtgactcca
       61 acactcccgg cgacgcaccg aaggacagcg gcggcggcag gcgccgcggc tgtttcggtt
      121 tctgctgaca ggtctcggcg agacccgaat gccgacagac gccgaacacg ccccgttcct
      181 gttcctctcg atccgcggag aggacgcggc ggcagacgac gaatatcagg cgatgatgcg
      241 cttcggcggg ctcgaccagc acgggatgca gcgaatccgc ctgacgcaca gggctttggg
      301 ggctgtcgat ctcgacgact ggtccggcat catcttgggc ggcgggccct acaatgcgag
      361 cgatccgccg gattcgaaga gctcgaccca gcggcgcgtc gaggccgagt tgctgtctct
      421 cgtcgatcgc atcgtggcgg cggacttccc gtttctcggg tgctgttacg gcgtgggcat
      481 gctcggttcg gttatcggtg ccaccgtgga tcgcagttac ccggaaccgg tggccgccgt
      541 caccgtcacg ctgaccccgg ccggccggga ggatccattg ttcgccgagc tgcccgacgt
      601 gttcgacgcg ttcggcggtc acaaggaggc ggccagctcg ctgccctccg ctgcggtgtg
      661 ccttgcgtcc tcacccgact gcccagtcca ggccttccgc gtcggcacca atacctacgc
      721 cacgcagttc caccccgaac tggacctaca gggcatcctc accaggatcg atgtctacaa
      781 gacctacggc tacttcgcgc cggagtccgc cgaatcgctg aaaacagcgg ccaggcaacg
      841 gcatatcgag tacccgccga cgatattgag gcggttcgtg caacggtaca gccggccgcg
      901 gtaggcgatc gcctcaggac ggatactcag gcgtccagtg gacctgcctg atgcggtccg
      961 ccagttgctc gtcgctgcgg gcgggtgcga ctccctccgc gatggcctgg cgaccgacgg
     1021 catgggcgat gtcttgagcg atgtcgggaa cgtccgacca cgcgggcagc agcggcgcag
     1081 caggatccga caaggcgggc gacgcatcgc cgagcgtggc ggccgcaacc cgcatcatgt
     1141 cgtcggtgac ccgggtggcc tgggccgcgg tgacggccag tcccatcgca gggaagatgt
     1201 agacgttgtt gcactgggcg atcggccgtg ccaccccgtc gcgctgcagc ggcgcgaagg
     1261 gcgaaccggt ggcgatgagc gcacgtccgt cggtccattg atccagctcg gccgggtgcg
     1321 cttcggcgtg gctggtcgga ttggacagcg gaaagatgat ggggcgctcc gtctttgccg
     1381 ccagctcgcg cacgatgctc tcggtgaagg cgcctgccgc ggtggacaat cccatcagga
     1441 tcccgacatc cacgttgtgc acgacgtcgg cgagctgggg cggccccgag aggccccaac
     1501 cggcgacgcg gcccgccggc tgggcgaacc tccgctgtgc cggcgacagg tcggtcctgt
     1561 cgtcggtgag caggccgatg acgtcgacaa cccagatccg ttccgctgcc tcggcttccg
     1621 acaaaccctg cgccaccatt tcgcgctgca ccattccgag cacgccgata ccggccgagc
     1681 cggcacccaa catcacgacc tgctgctgcg acagcgggcg gcctgcgact ttgga
//
LOCUS       DASYLX010000093         5423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3405600,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000093 DASYLX010000000
VERSION     DASYLX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405600"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VGP27_02265"
     CDS             <1..1344
                     /locus_tag="VGP27_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7850173.1"
                     /translation="SQPYPAWQELFNDILPAHIVKDVPGGFAAGLARALPVTGGQFRV
                     ENIDPQREEILLARNDRYWAEPAKPDQLLFRRAGATAALADSIRNGDTQVAQVHGGAA
                     AFAQLSAIPDVRTSRIVTPRVMQMTLRAEQPKLADPQVRKGILGLLDVDLLAAVGAGS
                     DNTVTLAQAQVRSPSDPGYTPTAPPALTKEAALNLLGNSGYQLVPLESPPTPTPGTPV
                     PDEDRGRITKDGVPLSLVIGVASNDPTSVAVANTAADQLRNVGIEASVLSLDPVGLYA
                     DAVTQNKVDAIVGWHQAGGDLATALSSRYGCRALEATAVSTATTGVTPTSTPSSTSHT
                     PNSPPTTATTTATPTTKSPAPESDELIQAPSNLTGICDRSIQPKIDAALRGTENIGEV
                     IDAVEPRLWNMSAVLPILQDTTIVAAGPRVQNVSLTGAVPVGIVGDAGMWAKKPQ"
     gene            complement(1454..2749)
                     /locus_tag="VGP27_02270"
     CDS             complement(1454..2749)
                     /locus_tag="VGP27_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV7850174.1"
                     /translation="MSRRTLEFCCGIIVVGLLAGIAGLCTTLLLRFVEHLTYHYSFGT
                     LLVGVTGSSPVRRAVGPMIGGALAGFGWWMLRRRTEVPPLADTIANHRPIPRLTMTID
                     AALQVLIVGSGASLGREGAPRQIAAALGDLGTSRWSLTERDREILLACAAGAGLAAVY
                     SVPVGGALFATAIMLKTWHPRAVGAALITSSLAVAVASPVTHEAAPLEWPSPQLSYLL
                     AAFALMLAPLALGVGIAFNRIMARARPATVMKSWVLMPAIAAAGLLIGICSIRYPELP
                     GNGKSILAVSLNSGLTLGAAVVILFLKPLLTALFLRAGAVGGMLTPALATGAALGSIV
                     ALAINSWTEHHVSIPAISLTCAAGVLAITQRAPIWAAIFVWELARPPWWLLIPFVVAA
                     VAAHWLRLLIEQRRLRDAAVDSPAGALREGGPGKRRQQR"
     gene            complement(2921..4546)
                     /locus_tag="VGP27_02275"
     CDS             complement(2921..4546)
                     /locus_tag="VGP27_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850175.1"
                     /translation="MQLALRPYVTTGMALVGASAIAVAPMIPTPTDVQIPNPARQVER
                     AVQLSAAEIEDTVNKLIFDFVASPTVDSAEALGKLLTPLIGAQQAFLLPIAALGLAGP
                     LISGGGAIGTALQEVVDSDNLEELLSSLIGAPGTILDGLVNGGYGPNLAPLVSAQVAA
                     VLSAALGKPIPPGAIGTVLAGGLLSEGGLLGLPKLPLGFELPGAIPTLEGLVEQLFGL
                     LGTTQTAGVNSLAVAAPDSTVEDGVNDLLFAATAATLRIVTLFAPLLEPILGDQAAAF
                     LALGTVGLLGPLISGTGATGNALQEIINSHGLAGLLGSLIGAPGTVADGVVNGGYGPN
                     LLPLLPALQAIPGATKAFAPGLIENPGFIYGLIPGGIGIKPTSATTLSVLPPGTIAVL
                     QGLVERVFGALPSAAGLNTTALKAPNVEQQDLKINGLAGDNDAKLTPGDNKSATDVDG
                     LGAVDGPGITPKKHRPRVELNVFKINPLAPDNTGDNGGAEADKDGGLTTTTAKHSLGI
                     GKTPVRDLVKRITGGLDHDNDTDDGGDATPAAS"
BASE COUNT         1004 a         1710 c         1741 g          968 t
ORIGIN      
        1 tcgcagccgt atccggcgtg gcaggaattg ttcaacgaca tcctgcccgc gcacatcgtc
       61 aaggacgtgc cgggcgggtt cgcagccggg ctggcgcgag cactgccggt tacgggcggt
      121 cagtttcgag tggagaacat cgacccgcag cgcgaggaga tcctgctggc acgcaatgac
      181 cggtactggg cagagcctgc aaagcccgac caactcttgt tccggcgtgc gggtgcgact
      241 gcggcactgg ccgattcgat ccgcaacggc gatacgcagg tcgcccaggt gcacggcggt
      301 gctgccgcgt tcgcacagct gtcggccatc ccggatgttc gtacgtcgcg catcgtcact
      361 ccgcgggtca tgcagatgac gcttcgcgcc gagcagccca agctcgccga tcctcaagtg
      421 cgcaaaggga tcctgggcct gctggacgtg gatttgttgg cagcagttgg tgcgggcagc
      481 gacaacacag tgacgctggc tcaggcgcag gtgcgctcac cgtcggatcc cggttacaca
      541 ccgacggcac cgcccgcgtt gaccaaagag gctgccctca accttttggg taacagcggt
      601 tatcagctgg taccactcga gtcgccgccg acgccgacgc ccggcactcc ggtgcccgat
      661 gaggatcgcg ggcgcatcac caaggacggt gtgcctttgt cgttggtgat cggcgtggcg
      721 tccaacgacc cgacgtcggt ggcagtcgcc aacacggctg ccgatcaatt gcgcaatgtc
      781 ggtattgagg cgtcagtgct atcgctggat cccgttgggc tgtatgccga tgccgtcacg
      841 cagaacaagg tcgacgcgat cgtcggctgg caccaggccg gcggcgacct cgccaccgcg
      901 ctgtcctcgc ggtacggatg ccgggcgctg gaagcgacgg cggtgtcaac cgcgacgaca
      961 ggcgtgactc cgacgtcaac tccgtcgtcg acgtctcaca cgccgaactc gccgcccacg
     1021 accgccacca cgacggcgac accgacgacg aaaagccccg cgccggagtc cgacgagctc
     1081 attcaggcgc cttccaacct gactggcatc tgcgatcgga gcattcaacc gaagatcgat
     1141 gctgcgctgc gggggactga gaacatcggt gaggtgatcg acgcggttga gcctcgcctc
     1201 tggaacatgt cggcggttct gccgatcctg caggacacca cgatcgtcgc ggccggccct
     1261 cgggtgcaga acgtcagctt gacgggtgca gtgccggtgg gcatcgtggg cgacgcgggc
     1321 atgtgggcca agaaaccgca atagcttccg aatagagatc ggctcccttg agagtgcccg
     1381 tcaccgtcct gatcgcacaa aggaccggcc gcggcatgtc cggcttagtc gcgcgccttt
     1441 gtcaccggcg ttgtcatcgt tgttgccggc gtttaccggg cccgccctcc cgtaatgcac
     1501 cggcaggaga atcgactgcg gcgtcacgca atctgcgctg ttctatcagc aggcgaagcc
     1561 agtgggctgc gaccgcggct accacaaacg gaatgagtaa ccaccatggc ggccttgcca
     1621 gttcccacac gaagatcgcg gcccagatcg gcgcccgctg agtaatcgcc aggaccccgg
     1681 ccgcacatgt tagcgagatt gccggaatgc tgacgtgatg ctcggtccag ctgttgatcg
     1741 ccaaggccac aatggatccc aaggccgcac cggtcgccag cgcgggggtg agcattccgc
     1801 cgaccgcccc ggcacgcagg aacagagcgg tgagaagtgg cttcagaaac agaatgacca
     1861 ctgccgcacc caaagtgagc ccgctgttga ggctcaccgc cagaatgctt ttcccattgc
     1921 ctgggagctc cggataccgg atcgagcaga tgccgatgag cagaccggcc gcggcgatcg
     1981 ctggcatcag aacccaggac ttcatcaccg tcgctgggcg cgcccgtgcc atgatccggt
     2041 tgaacgcgat ccccacgcct agagccaagg gggcgagcat cagtgcgaaa gcggccagta
     2101 gataagacag ttggggactc ggccattcaa gcggagcggc ctcgtgagtg acgggcgacg
     2161 ccacggccac cgcaaggctg gaggtgatca gcgcagcgcc gacggcgcga gggtgccagg
     2221 tcttcagcat gatggcggtc gcgaacagcg cgccgcctac tggcacgctg tagaccgccg
     2281 ccaaccctgc cccggccgca caggcgagca gaatctcgcg gtctcgctcg gtcagtgacc
     2341 atcgtgatgt cccgagatcg cccagtgctg cggcgatttg gcgcggcgct ccttcacgcc
     2401 cgagggatgc gcccgacccg acgatcagca cctgcagcgc agcgtcgatc gtcatcgtca
     2461 ggcgggggat cggccggtgg ttggcgatgg tgtccgccag tggcggcacc tcggtccgtc
     2521 ggcgaagcat ccaccacccg aacccggcaa gcgctccgcc gatcatcggg cccaccgcgc
     2581 gtcgcaccgg gctgctgccg gtgacgccaa ccagcagtgt cccaaacgag tagtggtacg
     2641 tcaggtgctc gacgaagcgc agcagcagcg tcgtacacaa accggcgatt ccggccagca
     2701 gtccgacgac gatgatgccg caacagaact caagcgtgcg ccggctcacg gagtgaatct
     2761 atgtcaagga tgtgcaggcg gacgtcgact tctgcgggag ggttgtggct gatccgccaa
     2821 ttgcagccct gaggcagatc tggggatgtt tggcgaagcg ctgctgcaag tatcagccgg
     2881 cccgggttgc cccgagccgg ctaatggatc cgcattcgcg ctagcttgcc gctggtgtcg
     2941 cgtctccgcc gtcgtccgta tcgttgtcgt gatccaagcc accggtgatg cgcttgacca
     3001 gatcgcgcac cggcgttttc ccaatgccga gcgaatgctt ggcggtggtt gtcgttaagc
     3061 cgccgtcttt atcggcctcc gcaccgccgt tatcgcccgt gttgtcggga gcgagcgggt
     3121 tgatcttgaa cacattcagt tcgacccgcg ggcgatgctt cttcggggtg atgccaggcc
     3181 catcgaccgc acccagcccg tcgacgtcgg tggcgctttt gttatcgccc ggcgtgagct
     3241 tggcgtcgtt gtcgccggcg agtccattaa tcttcagatc ttgctgctct acgttcggag
     3301 ctttcaaagc agtcgtattc aggcctgcag ctgacggcag cgcgccaaag acccgctcta
     3361 ctagcccctg taacaccgcg atggtgccgg gaggcaggac cgacaaggtc gttgcgctcg
     3421 ttggctttat gccgatccca ccggggatca ggccatagat gaaccctgga ttctcgataa
     3481 gtccaggggc gaaggccttg gtggccccag ggatcgcctg cagagcgggc agtagtggca
     3541 gcaagttcgg cccgtagccg ccgttgacca ccccgtcggc gactgttcca ggcgcaccga
     3601 tgaggctccc tagtaaccct gccaacccgt gggagttgat gatctcttga agcgcattcc
     3661 ctgtggcgcc tgtcccgctg atcagcggtc ccagaagtcc gaccgttccg agagccagga
     3721 aagccgccgc ttggtcgccc agaatgggtt ccagaagcgg cgcgaaaagc gtgacgatgc
     3781 gaagcgtcgc tgcggtcgcc gcgaagagca ggtcgttgac cccgtcttcg accgtcgaat
     3841 caggggccgc gactgccagg ctgttcacgc ccgccgtctg tgtggtgccg agcagcccga
     3901 acagctgctc gaccaggccc tcgagtgtgg ggatggcgcc agggagttcg aaacccagcg
     3961 gtagtttcgg aaggccaagg agcccgccct cactgagcag tcccccggcc aggacagtgc
     4021 caatcgcccc cggcgggatg ggcttgccaa gtgccgcact tagtacggcc gcgacctgcg
     4081 ccgacaccaa tggggcaagg ttgggaccgt acccaccgtt gaccaggccg tcgaggatcg
     4141 ttcctggggc accgataagg ctggaaagca gttcttccag gttgtcagaa tcgacgactt
     4201 cctgaagcgc agtgccgatg gcgccgccac cgctgataag cgggccggcc aggcccaacg
     4261 ccgcgattgg gagcaagaaa gcttgttgtg ctccgatgag aggcgtgagg agcttcccta
     4321 gggcctccgc actatcgacc gttggactcg caacgaagtc gaaaatcagt ttgttgaccg
     4381 tatcttcgat ctccgcagcg gagagttgca ctgctcgctc cacttgcctc gccgggttcg
     4441 gaatctgaac gtcggtcggt gtggggatca ttggggcaac agcgatagcg cttgccccca
     4501 cgagggccat accggtcgtg acgtaggggc gcagagcaag ttgcatggat tcctccagat
     4561 cggggctgct gtgacgtgca gcactcagga atctatggca gaaaattcat ctgattctca
     4621 ggttcagtgg acggcgcttc aagatagttg caatcgcaat aacttgcctg atggcaacgg
     4681 agtagctggt cggctgggcg gattggctaa gttgtggccg gggcggtttc tgggcaaccc
     4741 acggcagatc ggtcgtggcg cgatccggca tgtatgactc gttgaggatc cgtatcaggc
     4801 gcgaggcgag tagacagcca agtcaaatgc cttgtcccag tgcgagtctc acaactgggg
     4861 cgggtgcaag gatgggacct gctcggcgag tgcccgtcat cgatgccgag atggtcgaca
     4921 gagctcgcct tcgccacatg cggcccaccg gacacagcgg agcaacgtcg gtcaccagaa
     4981 cgcccgaacg ggtgtgtccc ttaactgacg acattgtcaa tacggtggac gaaggctgat
     5041 tcagctgtca tcgcgacacg acggcccgcc tcgcggccac ccgggatgcg aaagctaggc
     5101 ctccctggac atgattcggc cccgctgtcg gtgggtgtga gggcaccaac agcggggccg
     5161 atatggaagg cgatgagtta cctcagcgac ggctcccctc cgtcacccgg acccccaagc
     5221 ccgggctgtt cgccttctgc gtcatgttga gttttcactg caactcatga ttgctgcgcc
     5281 ctctgtcagc gaggccgcga tctgagaagt catcgttcgt ctttattgtc gctatgacgc
     5341 acgaatctat tacatatctg gagcgaccct aatcacgatc ccgcaatctc gttgcgaaca
     5401 caagcacatg ccgtgaactg ctt
//
LOCUS       DASYLX010000094         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_662935,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000094 DASYLX010000000
VERSION     DASYLX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662935"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..589)
                     /locus_tag="VGP27_02280"
     CDS             complement(8..589)
                     /locus_tag="VGP27_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEV7850176.1"
                     /translation="MKLRKEAHFSRPWRIHELTPGFRLEDVWALPTVGGADDFPRLIQ
                     MVASFDEAQQRVSVTGFLFAVRQVLGRAFGWDSTSDDPSRPGLRQTLPADLRHSPSTV
                     RPAMGFTALYQLDDEWAAELINQTVHGVIHLGWVGDEAGGYRGQMAILVKPNGRLGRA
                     YMAAIMPFRYLIVYPQMLKQIGRRWLSQSRIRP"
     gene            625..1251
                     /locus_tag="VGP27_02285"
     CDS             625..1251
                     /locus_tag="VGP27_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850177.1"
                     /translation="MHVSIPESEGRPRTQAERRARTRAALLEAAAKGFSRYGYGNLVL
                     EKVAADAGYTRGALYHLFRGKEELAVAVVDWVAATWEREVWEPAQRAGAPVDILVALA
                     RDHIVFCRRDIARVMMALRVEFDGRVHPVGTRVADIGKDLAMRFGDVVAAGRDDGSIP
                     PGPPAKTLGAAITAAVEGLAIHLAGAAHDEEMAERMVRGLLGTLEPCN"
     gene            1242..2189
                     /locus_tag="VGP27_02290"
     CDS             1242..2189
                     /locus_tag="VGP27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV7850178.1"
                     /translation="MQLITIEDVRAAADRIKDAVVRTPLIPAPWAGAERPLWIKPENL
                     QPIGAFKIRGAFNAIGNLEETDRARGVVAYSSGNHAQAVAYAAAAFGIEAHIVMPRET
                     PHVKVQATRGHGAHVVLCEAGEREKVAAEVLEQTGGILIPPFDHPDVIAGQGTIGLEI
                     ADDLPEVENVLVPVSGGGLASGIGIAIKARCPWAKVFGVEPELAADTAESLRRGERVD
                     WSIEDRNRTIADGLRSQPSALTFAHLQKVLDGLITVSENEIRAAVRDLAVNAHLVSEP
                     SGAVALAAYRQGVTPPGRTVMVLSGGNVGPAVLSDILLD"
     gene            2394..3704
                     /locus_tag="VGP27_02295"
     CDS             2394..3704
                     /locus_tag="VGP27_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850179.1"
                     /translation="MDAVLGLSMTPTTVGLVLVEGQDADGATMDKDEFAVHDRGLVSA
                     VNTSEKAAAAVMRTEAIAAARGHRLHSIGVTWSEDADTEASLLLESLSESGFDNVVAV
                     RLPQATEALARGIAGVIGHEITAVCVIEPDAVVVLVVDTADGAVQTAVNHAVSTDEDL
                     ARWLSAIFAQAEWQPEALVLVGSASDLEAITPHLEEVLAVPVFVPAEAQLALARGAAI
                     ASTHGPDYFVDQAGDESDQTRPRRTAPFPRIGAAGAMLGVGVVTFVVSGSLAIGLHLS
                     SQESAKPAEKPPAAAAVKAVQPAPAASPTIEAALPPPPPPAEPPPPPVYDVEPVPIAD
                     PLAPEAPAPDPLAPADGSLPQGPLAPNGVPLPPGVTDPAPGLVPAPVTPVPEQSGGFL
                     GRIRDRLSDIGKPDQPAQAPVDVAPPQDPPLVPPPADAPPPPAG"
     gene            3679..4299
                     /locus_tag="VGP27_02300"
     CDS             3679..4299
                     /locus_tag="VGP27_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV7850180.1"
                     /translation="MRPRHRPDNDFVRVLLRVLILAAVVLALISVETTSRSGVLWRLI
                     TFTYQANVLAAAYYSWSLFSARADDRGGVRGAVVLYVVVAGIIWNLLLTDYSMGYTPA
                     NVLLHIVVPALVTMDWLVVGRGQRSVRWWQPLVWLIYPAAYVVLALLVLNRAGRRAPY
                     YFLDPDSVGMAVVVTNIGLLAVFILVLGYAISVVGRSRRAGPPPVG"
     gene            complement(4296..4964)
                     /locus_tag="VGP27_02305"
     CDS             complement(4296..4964)
                     /locus_tag="VGP27_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV7850181.1"
                     /translation="MTVSAERAVRKGFTLRDARREFVKHPSPWVIGTALVAALTARIV
                     VGDWQKSDALVPVAMLALFPFFEWVVHVFVLHWRPKRIGRLTIDPLLAREHRRHHIDP
                     RNVPLIFIPWKALLWAAPLALAVALLGFPRVGLGLTGLVFLMALGLGYEWTHYLVHSD
                     YKPRSGFYRAIWRNHRQHHFKNEHYWFTVTSSGTADRVLGTYPDPTVVETSPTAKNLH
                     AIEA"
     gene            complement(4961..5683)
                     /locus_tag="VGP27_02310"
     CDS             complement(4961..5683)
                     /locus_tag="VGP27_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7850182.1"
                     /translation="MALKPVNRRSVPEDVFEQILADVLSGEMQPGETLPSERRLAEVL
                     GVSRPAVREALKRVSAAGLVEVRQGDATTVRDFRRHAGLDLLPRLLLRGGELDLAVVR
                     SILEARLHNGPKAAELAAVRRPPGLAALLDETIGSLESETDAVEQQRHALTFWDHVVD
                     GADSIVFRLMYNTLRATYEPALPALAAVMAAEVGRPQAYRELAAAIAAGDPDAARKAA
                     TDLLEPATAAMLAVISSLEEHR"
     gene            5719..6008
                     /locus_tag="VGP27_02315"
                     /pseudo
     CDS             5719..6008
                     /locus_tag="VGP27_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5959..>6193)
                     /locus_tag="VGP27_02320"
     CDS             complement(5959..>6193)
                     /locus_tag="VGP27_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="steroid C27-monooxygenase"
                     /protein_id="HEV7850183.1"
                     /translation="FNVLRDPNPHVGFGGTGAHYCIGANLARMTIDLIFNAIADEMPN
                     LTPLSKPERLRSGWLNGIKHWQVDYTGKTAAAT"
BASE COUNT         1010 a         2004 c         2121 g         1058 t
ORIGIN      
        1 gccgcggcta cggccggatg cggctttgcg aaagccatct cctgccaatc tgtttgagca
       61 tttgcggata gacgatcagg tagcggaacg gcatgatggc cgccatgtac gcgcggccga
      121 gacggccgtt gggcttcacc agaatggcca tctggccgcg atatccaccc gcttcatctc
      181 ctacccagcc gaggtgaatg acgccgtgca ctgtctggtt gatgagttcg gcggcccact
      241 cgtcgtcgag ctggtacaga gcagtgaagc ccattgccgg ccgcaccgtg gagggggagt
      301 ggcgcagatc cgcgggcagc gtttgccgaa ggcccggacg cgagggatcg tcggaggtgc
      361 tgtcccagcc gaaagcccga ccgagcacct gccggaccgc gaagagaaac ccggtcaccg
      421 aaacccgctg ctgcgcctcg tcgaaagagg ccaccatctg gatcagccgg gggaagtcat
      481 cggccccgcc tacggtcggt agcgcccaga catcctcgag gcggaagccg ggggtcagct
      541 catggatgcg ccacggtcgc gaaaagtgag cctctttacg gagtttcatc ggacacacct
      601 ccggtctgac gctaacatac atacatgcac gtatctatac ccgaatccga gggccgcccg
      661 cggacccagg cggagcgccg cgcacgcacc agggcggccc tcctcgaggc ggcagccaaa
      721 ggtttctcgc ggtacgggta cggcaacctc gtgctcgaga aggtggcagc cgatgccggc
      781 tacacccgcg gagcgctcta ccacttgttc aggggcaagg aggaactcgc cgtcgccgta
      841 gtggactggg tcgcggccac ctgggaacgg gaggtctggg aacccgcgca gcgcgcgggt
      901 gcgcccgtcg acattctggt ggcgctcgcg cgggaccaca tcgtgttctg ccgccgcgac
      961 atcgcgcgcg tgatgatggc gctacgggtc gagttcgacg gccgtgtaca cccggtcggg
     1021 accagggtcg ccgacatcgg gaaggacctt gcgatgaggt tcggggacgt cgtcgccgcg
     1081 ggcagggatg acggatcgat acctcccggc ccgccggcga aaacgctcgg tgccgcgatc
     1141 accgccgccg tcgagggtct ggcgattcac ttggccggcg ccgcgcatga cgaggagatg
     1201 gcggagcgca tggttcgcgg gttgcttggc acactcgaac cgtgcaactg atcactatcg
     1261 aggacgtccg agcggcggcc gaccggatca aggacgccgt cgttcgaacc ccgctgattc
     1321 cggcgccatg ggccggcgcg gagcggccgc tgtggatcaa gccggagaac ctacagccga
     1381 tcggcgcgtt caagatccgc ggcgccttca acgcgatcgg caacctggaa gaaaccgacc
     1441 gggcgcgagg cgtggtcgcg tactcgagcg gcaaccatgc ccaggcggtg gcctatgccg
     1501 cagcggcgtt cgggatcgaa gcacacatcg tcatgccgcg agagacaccg cacgtcaagg
     1561 tgcaggccac ccgtggccac ggcgcacacg tcgtgctgtg tgaggcgggc gaacgcgaga
     1621 aggtcgccgc ggaggtgttg gagcagaccg gcggcatcct gataccgccc ttcgaccacc
     1681 cggacgtgat cgcggggcag ggcacgatcg gcctggagat cgccgacgac ctaccggagg
     1741 tggagaacgt gctcgtaccg gtcagcggcg gcgggctggc ctcgggaatc ggaatcgcaa
     1801 tcaaggccag gtgtccctgg gccaaggtct tcggggtcga gccggaactg gcggcagaca
     1861 ccgccgagtc ccttcgacgc ggtgaacggg tggactggtc catcgaagac cgcaaccgga
     1921 ccatcgccga cggccttcgc tcgcagccgt ccgcgctgac gttcgcacac ctgcagaagg
     1981 tgctcgacgg tttgatcacg gtgtccgaga atgagattag ggccgcggtt cgagaccttg
     2041 ccgtcaatgc gcacctcgtc agcgagccaa gtggcgcggt ggcactggcg gcctaccggc
     2101 agggcgtgac gccacccggc cgcacggtga tggtgttgtc cggcggcaac gtcggaccgg
     2161 ccgtcctttc cgacatcctt ctggactgag aaattactca gcaacttcat actgtttgct
     2221 gcttgggcgt acgtcggcgg tcgaaacgcc attcgccgga tacttgattt gctaggcgtc
     2281 aagccgtcga cggtggcagc ggtcccgggg cgtcctcacc acgagatcca tgtagtctca
     2341 ccccgttagg tgacgaagac cacgcccgta atagatgtcc cgagggggtg cggatggacg
     2401 cggtgctcgg actgtccatg acgccgacca ccgtggggct cgttctcgtc gaaggccaag
     2461 acgctgacgg cgccaccatg gacaaggacg agttcgcggt tcacgaccgg ggccttgtca
     2521 gcgccgtgaa cacttccgag aaggctgcgg ccgccgtgat gcggaccgag gcgatcgccg
     2581 cggcacgcgg tcaccggctt cattcgatcg gggtcacgtg gagtgaggac gccgacaccg
     2641 aagcttcgct tctcctggag tcgttgtcgg agtcgggctt cgacaacgtc gtcgcggtgc
     2701 gactgcccca ggccaccgag gcgctggccc gcggaatcgc aggagtcatc ggtcacgaga
     2761 tcaccgccgt gtgtgtcatc gaacccgatg ccgtcgtcgt gcttgtggtc gacacggccg
     2821 acggcgccgt gcagacggcg gtcaatcacg ccgtcagcac cgacgaggac ctcgcccggt
     2881 ggctgagcgc gatcttcgcc caggccgagt ggcaaccgga ggccttggtg ttggtcgggt
     2941 cggccagcga cctcgaagcg attaccccgc atctcgaaga ggtgctggcg gtgccggtct
     3001 tcgtccctgc cgaggctcag ctggcacttg cccgtggcgc ggccattgcc tcgacccacg
     3061 gacccgatta cttcgtggac caagccggcg acgaatcaga ccagacaaga cctcgtcgga
     3121 cggcgccatt cccgcgtatc ggggccgccg gcgccatgct gggtgtcggc gtggtgacct
     3181 tcgtggtttc gggatctctg gcgatcggtc tgcatctgtc ctctcaagag agcgcgaaac
     3241 cggccgagaa gccgccggcg gctgccgcgg tgaaggctgt gcagccggct cctgccgcct
     3301 cgccgacaat cgaggcggca ctgcctccgc cgcctccgcc cgctgaaccg ccaccgccgc
     3361 ccgtatacga cgtcgaaccg gtcccgatcg ccgatccact ggcgccggag gccccggctc
     3421 ccgatccgct ggcaccggcc gacggatcgc ttccgcaggg tccgcttgcc cccaatggcg
     3481 tgccgcttcc cccgggtgtc accgacccgg ccccgggtct ggtgcccgca ccggtgacgc
     3541 cggtgccgga gcagagtggg ggattcctcg gacggatccg agacagactg tcggatatcg
     3601 gcaaaccgga ccaaccggcc caggcgccgg tcgacgtggc gccacctcag gatccgccgc
     3661 tggtgccgcc gcccgcggat gcgcccccgc caccggccgg ataatgactt tgtgcgggtc
     3721 ctgctacgcg tcctgatcct cgcggcggtg gtgctcgcgc tgatctccgt cgagacaacg
     3781 tcacgatcgg gtgtgctctg gcggctgatc accttcacct atcaggccaa tgtgcttgcg
     3841 gcggcgtact actcgtggag tctgttctct gcgcgcgcag atgaccgagg cggcgttcgg
     3901 ggggccgtcg tcctctatgt cgttgttgcc gggatcatct ggaacttgct gctgaccgac
     3961 tacagcatgg ggtacacccc cgctaacgtc ttgttgcaca tcgtcgttcc ggccctcgtc
     4021 accatggact ggctggtggt cggacgaggt cagcgcagcg tccgctggtg gcagccgctg
     4081 gtgtggctga tctatccggc ggcctacgtt gtgctcgcgt tgctcgtgct caacagggcg
     4141 gggcggcgtg ctccgtatta cttccttgac cccgactccg tcggaatggc cgttgttgtc
     4201 acgaacatcg gcttactcgc ggtgttcatc ctggtgctgg gatacgcgat ctcagttgtc
     4261 ggcaggtcta ggcgagcagg tccgcccccg gtcggttaag cctcgatcgc gtgcaggttt
     4321 ttggcggtcg gcgacgtctc gacaacggtg gggtcggggt aggtgcccaa cacccggtcg
     4381 gcggtgccgg agctggtgac ggtgaaccag tagtgctcgt ttttgaagtg gtgctgtcgg
     4441 tggttgcgcc agatcgctcg atagaagcca gatctcggtt tgtaatcgct gtgcacaagg
     4501 taatgggtcc actcgtatcc caggccgagc gccatgagga aaaccaagcc tgtcagcccg
     4561 agcccaactc gcggaaagcc aagcagcgca acggccaaag cgaggggagc cgcccagagc
     4621 agagccttcc acggtatgaa gatcagcgga acgtttcggg gatcgatgtg gtggcgccga
     4681 tgttcgcgcg caagcagcgg gtcgatggtc agccgaccga tgcgcttcgg ccgccagtgc
     4741 agaacgaaca cgtggacgac ccattcgaag aacgggaaga gggcgagcat cgcaaccggt
     4801 accagtgcgt cggatttctg ccagtcgcct accacgatgc gcgccgtcag cgcagccacc
     4861 agcgcggtgc cgatcaccca gggcgaaggg tgtttgacga actcgcgccg tgcatcgcgc
     4921 agagtgaatc ccttgcgtac tgcgcgttcg gcggagacgg tcatcggtgt tcctccagac
     4981 tgctgatcac cgcgagcatc gctgcggtgg cgggttcgag tagatccgtc gccgcctttc
     5041 tggcggcgtc agggtcgcct gcggcgatcg cggcggcgag ttcccggtag gcctgcgggc
     5101 ggccgacctc ggcggccatc acggcggcga gagcaggcag cgcgggctcg taggtggccc
     5161 gaagcgtgtt gtacatcagc cggaagacga tggaatctgc tccgtcgacg acgtggtccc
     5221 agaacgtcag cgcgtggcgt tgctgctcga cggcgtccgt ctcggattcc aggctgccga
     5281 tggtctcgtc gaggagcgcg gccaagccgg gtgggcgccg cacggcggcg agttcggcgg
     5341 ctttcggtcc gttgtgcaat cgcgcctcca ggatgctgcg aacgaccgcg aggtcgagtt
     5401 cgcctccgcg gagcagcagc cgggggagca ggtcgagacc ggcgtgccta cggaagtcac
     5461 gcaccgtggt cgcgtcccct tggcgcacct caaccagacc ggcggccgat acccgtttga
     5521 gcgcctcccg cacggcggga cgggacactc cgagcacttc ggcgaggcgg cgctcgctgg
     5581 gcaaggtttc tcctggctgc atctcgccgc tgagcacgtc ggcgaggatc tgctcgaaga
     5641 cgtcctcggg caccgaacgc cggttcaccg gttttagggc catggaccca ccctggccga
     5701 tcaaaggcca gaggtcaagt ggtcagacca gctactcctg cgtcctggta catcggcacc
     5761 ggcttcttct tcgcgccggt gctcgcggtg ctctcgccct ggcccacgct caccgcgatc
     5821 ctgtgggcgt gatcgccctc gcggggctgt ggctggcgct gctcggcatc gcaatggcga
     5881 ccgggctcgc gatcgtcttg cggtcggggg aggagatctc cgaggactac tggcgcacga
     5941 tcgtcgacta cccggcggct acgtcgcggc agccgtcttc ccggtgtagt ccacctgcca
     6001 gtgcttgatg ccgttgagcc atcccgaccg aagccgctca ggcttggata acggcgtcag
     6061 attgggcatt tcgtcggcga tcgcgttgaa gatcagatcg atcgtcattc gcgccaggtt
     6121 cgcgccgatg cagtagtgcg caccggtgcc gccgaacccc acgtgcgggt tgggatctcg
     6181 cagcacgttg aag
//
LOCUS       DASYLX010000095         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_882599,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000095 DASYLX010000000
VERSION     DASYLX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882599"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2351
                     /locus_tag="VGP27_02325"
     CDS             21..2351
                     /locus_tag="VGP27_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7850184.1"
                     /translation="MLHGLTRLAISAPRRVLAIAILVIVGCAVFGVPVAKSLSGGGFR
                     DPTSESARASAVLADTFDRPDSQFLLIVSSDRGVRDLQVEAVGTDIVGQLKETPHVAF
                     VESPWSSSPGAAAGLISKDGKSALIAAGLTGDEGDTATYAADLSDRLVHDHDGITIKA
                     GGSAVVNAQINKQTERDLLLMESIAIPLSFLVLVWIFGGLIAATLPMMVGAMAIFGTM
                     TVLRLTTFVTDVSIFALNLATAMGLALAIDYTLLILSRYRDELADGSPREEALRRTMA
                     AAGRTVLFSALIVGLSMLPMALFPMYFLKSFAYAGISVVAFAACAALVVTPAAITLLG
                     ARLDALDVRRLLRRALRRPQPVTPPVERTFWYRTTKSVTRRAVPFGLAVVTFLLLLGT
                     PFLGVHWGFPDEQVLPKSASAHRVGDELRENFAINLATAVTVVLPDVGKVTPQELDRY
                     GADLSKVPGVPFVSGPGGTYVAGQRIGPPSAPAGLADGKAYFTVASTTSVASEASEHQ
                     LDQLHDVARPGGAQVQMAGIEQSNRDIVHAVTSGLPAVLGLIAATTFIMLFLLTGSVV
                     LPLKALIMNVLSLTAAFGGLVWIFQDGHFGGFGTSVTGTLAVTMPVLLFCIAFGLSMD
                     YEVFLLSRIREYWLSSDQTRADNDEAVALGLGHTGRVVTAAAVIMSISFAALIAAQVS
                     FMRMFGVGLTLAVLMDATLVRLVLLPAFMHVMGRGNWWAPAPLVRLHKRIGISESPPP
                     GRHARPEAEVTARHPQADVGEEPAIEPPVDVISPSR"
     gene            2448..3320
                     /locus_tag="VGP27_02330"
     CDS             2448..3320
                     /locus_tag="VGP27_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV7850185.1"
                     /translation="MTEDGAGADPPAQAGLTGRPPRMIPEPTPKNVHGPAKVIAMCNQ
                     KGGVGKTTSTINLGASLAGYGRRVLLVDLDPQGALSAGLGVPHYELDHTVHNLLIEPR
                     VSVDDVLIHTRVKNMDLVPSNIDLSAAEIQLVNEVGREQSLARALHPVLDRYDYVLID
                     CQPSLGLLTVNGLACSDGVIIPTECEYFSLRGLALLTDTVDKVHDRLNPKLSISGILI
                     TRYDPRTVNAREVMARVLERFGDLVFDTVVTRTVRFPETSVAGEPITTWAPKSGGAQA
                     YRSLAREVIDRFGS"
     gene            3317..4150
                     /locus_tag="VGP27_02335"
     CDS             3317..4150
                     /locus_tag="VGP27_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV7850186.1"
                     /translation="MNTQTGPAEAQQTGFRVRLSNFEGPFDLLLQLIFAHRLDVTEVA
                     LHQVTDDFIAYTKAIGPQLELDETTAFLVIAATLLDLKAARLLPAGEIHDEEDLALLE
                     VRDLLFARLLQYRAFKHVAEMFAELEAAALRSYPRAVSLEDRYEDLLPEVMLGVDAQL
                     FAEIAASAFTPRPVPTVATEHLHEVMVSVPEQAEQLLAELQRRGSGQWATFSELVADC
                     REPILIVGRFLALLELYRARAVAFEQSEPLGVLQISWTGDRPSSEEIEATETVEEPND
                     D"
BASE COUNT          689 a         1360 c         1352 g          789 t
ORIGIN      
        1 ccgccgagga agggcgagac gtgcttcatg gcttgacgag acttgccata tccgctccga
       61 ggcgcgtgct cgcaatagcg atcctggtga ttgtcggctg tgcagtcttc ggcgtgccgg
      121 tggccaagag cttgtcaggc ggcgggtttc gtgacccgac atcggaatcc gctcgagcct
      181 cggcagtgct ggcggacacc ttcgaccgac ccgactcaca gttccttctc atcgtcagct
      241 cagatcgcgg cgtgcgcgat ctgcaggttg aggcggtggg cactgacatc gtcggacagt
      301 tgaaggaaac gccacatgtc gcattcgtgg aatcgccgtg gagcagttcg ccgggcgccg
      361 ctgcgggact gatcagcaag gacggcaagt cagcgctgat cgccgccgga ctgaccggtg
      421 acgaaggcga cacagctacc tatgcagcgg acttgtcaga ccgcctggtg cacgaccatg
      481 acgggatcac catcaaagcc ggcggatcgg cggtcgtcaa tgcccaaatc aacaagcaga
      541 ccgagcggga tcttttgttg atggagtcga ttgcgattcc gctgagcttt ctcgtcctcg
      601 tctggatttt cggcgggcta attgccgcga cgctgccgat gatggttggg gcgatggcca
      661 ttttcgggac catgaccgtg ctgcgcctga cgacgttcgt cactgacgta tccatcttcg
      721 cgctcaatct cgcaacggcc atggggctgg cgctggccat cgactacaca ctgctgatcc
      781 tctccaggta ccgcgacgaa ctcgccgacg gctcaccccg cgaagaggcg ttgaggcgca
      841 ccatggcagc cgcgggtcgt acggtgttgt tctcggcgtt gatcgtcgga ctttcgatgc
      901 tccccatggc cttgttcccg atgtacttcc tcaagtcgtt cgcgtacgcc ggcatctcgg
      961 ttgtggcttt cgccgcgtgc gctgcgcttg tcgtgacgcc ggccgccatc acgctcctgg
     1021 gcgcgcgcct cgacgcgttg gatgtgcggc ggctgcttcg gcgggcgctg cgccgtcctc
     1081 aaccggtgac cccgccggtg gagaggacct tctggtaccg caccaccaag tcggtgactc
     1141 gccgagctgt tccgtttggg ttggcggtcg tgacctttct gctgctgctc ggcacaccat
     1201 tcctcggagt gcattggggg ttccccgatg agcaggtgct gcccaagtcg gcatctgcac
     1261 atcgcgtcgg tgacgaactg cgcgagaact tcgcgatcaa cttggcgacc gcagtcaccg
     1321 tcgtcctgcc tgacgtcgga aaggtcacgc cccaagagct cgaccgctac ggcgccgacc
     1381 tgtccaaggt tcccggcgtg cccttcgtct cggggccggg cggcacctac gtggccggac
     1441 agcggatcgg tccgccctcg gcgcctgccg ggctcgctga cggcaaggcg tacttcaccg
     1501 tcgccagcac gacatcggtg gcttccgagg cgtcggagca ccagctggat cagctacacg
     1561 atgtcgcgcg gcccggcgga gcccaggtgc agatggcggg tatcgagcag agcaatcgtg
     1621 acattgtcca cgcggtgacc agcggactcc ctgcggtact cggtctgata gccgcgacca
     1681 cgttcatcat gctgttcctg ttgacaggga gtgtggtcct cccgctcaag gcgctgataa
     1741 tgaacgtctt gtcgctcacc gcagcattcg gcggcctggt atggatcttc caggacgggc
     1801 acttcggggg cttcgggacc tcggtcaccg gcacactcgc cgtcacgatg cccgtgctgt
     1861 tgttctgcat cgcattcggt ctgtcgatgg attacgaggt gttcttgctc tcacgaatcc
     1921 gcgaatactg gctcagctcc gatcagaccc gcgcggacaa cgacgaagcg gtggcgctgg
     1981 gactgggcca taccggccga gtggtcactg cggcagcggt gatcatgtcc atctccttcg
     2041 ctgcgctgat cgcggcccag gtgtcgttca tgcgcatgtt cggcgtcggg ctgaccttgg
     2101 ccgtgttgat ggacgcgaca ctggtgaggt tggtgctgct gcctgcgttc atgcacgtca
     2161 tgggtcgggg caactggtgg gcgcccgcgc cgctggtccg attgcacaaa cgcatcggga
     2221 tcagcgagtc ccccccgccg ggtcgacacg ctcgaccgga agcagaggtc acagcccgcc
     2281 accctcaagc cgacgtcggc gaggagcccg ccatcgagcc ccccgtcgac gtaataagcc
     2341 ctagtcgatg atccgcccgc gggtctgcct gtccaggccc ccgcgccccg ttaaactttc
     2401 ccgatgttcg ccatggccct gcgttgtctg accgctagag ccgaggcatg actgaggacg
     2461 gcgccggcgc cgacccgccc gctcaagcgg gactgaccgg tcgtccaccg cggatgatcc
     2521 ccgaacccac gcccaagaac gtgcacggac cggcgaaagt catcgcgatg tgcaatcaga
     2581 agggcggcgt cggcaagacc acctcgacca tcaacctggg cgccagcctt gccggatacg
     2641 gccggcgggt gttgctggtg gacctggacc cgcaaggtgc gctgtcggcg gggcttggtg
     2701 tgccgcacta cgagctcgac cacaccgttc acaacctgct gatcgagccg cgcgtctctg
     2761 tcgacgacgt cctcatccac acgcgggtca agaacatgga cctcgtgccc agcaacatcg
     2821 atctgtcggc ggcggagatc cagctggtca acgaggtcgg ccgggagcag tcgctggcac
     2881 gtgcgctgca tccggtgctg gaccgctacg actacgtgct gatcgactgc cagccgtcgc
     2941 tcggcctgct caccgtgaac gggctggcct gttcggacgg cgtcatcatc ccgacggaat
     3001 gcgaatactt ctcgctgcgc gggttggcgc tgctgaccga caccgtcgac aaggttcacg
     3061 acaggctgaa ccccaagctg agcatcagtg gcatcctgat cactcgctac gacccgcgta
     3121 ccgtcaacgc ccgcgaagtg atggcccgcg tcctggagag gttcggtgat ctggtgttcg
     3181 acactgtcgt cacccgtacc gtgcgcttcc cggaaaccag tgtcgcgggc gagcccatca
     3241 ccacgtgggc gcccaaatcc ggtggcgctc aggcgtatcg atcgttggcc cgtgaggtca
     3301 tcgaccggtt cggttcgtga acacgcagac tggaccggcg gaggcgcagc agacaggctt
     3361 ccgggttcgg ctgagcaatt tcgagggtcc gttcgacctg ctgctgcagc tgatcttcgc
     3421 gcaccgtctg gacgtgaccg aggtggcgtt gcaccaggtg accgacgact tcatcgccta
     3481 cacgaaggcg atcggtcccc agctcgagct cgatgagacc accgccttcc tggtgatcgc
     3541 tgcgacgctg ctcgacctga aggccgcgcg tttgctgccc gcaggcgaga tccacgacga
     3601 ggaggacctt gccctgctcg aggtgcgcga cctgctgttc gcgcgtctgc tgcagtaccg
     3661 cgcgttcaag cacgtggccg agatgttcgc cgagctcgag gcggccgccc tacgcagcta
     3721 tccgcgggcg gtatcgctgg aagaccgcta tgaagacctg ctccccgagg tgatgctcgg
     3781 cgtcgacgcg cagctgttcg ccgagattgc cgcgagcgca ttcaccccgc gcccggtgcc
     3841 gacggtggcc accgagcatc tgcacgaggt aatggtgtca gtgcccgagc aggccgaaca
     3901 actgctggct gagctgcaac gccgcgggtc ggggcagtgg gcgaccttct ccgagctggt
     3961 cgccgactgc cgggaaccga tcttgatcgt gggtcggttc ctggcgctgc tcgaactgta
     4021 ccgagcacgg gcggtagcat ttgagcaatc agaaccgctt ggtgtgctgc agatttcgtg
     4081 gaccggagac cggccatcca gcgaagagat cgaagccact gagacggtgg aagagcctaa
     4141 cgatgactga cgaatattcc gatccgcgca acggcgatgc ccgcccggtc
//
LOCUS       DASYLX010000096         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1759934,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000096 DASYLX010000000
VERSION     DASYLX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759934"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1063
                     /locus_tag="VGP27_02340"
     CDS             293..1063
                     /locus_tag="VGP27_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEV7850187.1"
                     /translation="MVAACSGNANPSRQPGRAEHRATPTAKPPATPSAADGACPKTTP
                     PETAGPHPADGSNGPNVLGEEGVVRSDIRSSFGAVSGWAEGVQTTIELTVLDVTRDCA
                     KGEGMAVYVWHCDRAGDYSLYSGDAADKNYLRGVQVAGQDGRVSFTSVFPACDPGRWP
                     HIHFEVFDSLDAAVAGVNARLTSQLALPEGACKKVFDYDKGYSASVQNMSNVTPEKDS
                     VFGDGWDGELATVTGDPSSAMLVTLTIGVGEKPSPPPP"
     gene            1223..2497
                     /locus_tag="VGP27_02345"
     CDS             1223..2497
                     /locus_tag="VGP27_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7850188.1"
                     /translation="MLLSWSRRARIGSVATVVAAVVLLAGDVADTTAQCSTQCQVASA
                     AQTIAKPEPAPPQPAAIGVFPQAQADDVNPAAPVSVAAFSGSIVNVEMVNDNGTTVPG
                     ALTPDKKGWHPTEQLGYGRTYTMTVVARGPGGMPSRQTSSFTTLTPDYETAVNLNTIA
                     GVSLVDGTTYGVGTVVVAHFDEAITDKVAAEKRLHVTTNPPVAGVWNWVDDQEAHWRP
                     EKYYAPGTAVDVTADIYGAELGDGMYGQDDQHVAFKIGNAHVSIADDTTKQVSVFDNG
                     KLVRTMPTSMGMGGTQTIGGTTLSFWTPPGTYTVLDKANPVIMDSSTFGLPTGSRLGY
                     RETIPYATRISMDGIYLHQLNATVWAQGNTNTSHGCLNLNGENAAWFFDFSVPGDVVE
                     VHHTGGPALTVKQGGDWTVPWKEWRQGSALNA"
     gene            complement(2487..>3156)
                     /locus_tag="VGP27_02350"
     CDS             complement(2487..>3156)
                     /locus_tag="VGP27_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV7850189.1"
                     /translation="GHLALWLGLLAAIVALTTAMGQIERGANRIYGIERDRPALRKYG
                     RALLMAMTAGLLLQLGFLVIIAGDALGEALAAAYGWGDTFQSLWTIGSWPLGVLLALA
                     SFTVVIQRAPRRRQPGYSWLAFGAGVSLLLWLLLTGLLALYVVKSGSFGSTYGPLTGI
                     FALLLWANLSSVALFLGIAFAAQLEAVRAGAPEPTTGDPLDGPSSAPRAQRTVEEPGM
                     AVTR"
BASE COUNT          576 a         1139 c          989 g          452 t
ORIGIN      
        1 ccctcgcgct cgtaccgctg gcaggaccag tcgaactcgg tgtaggcaag atcgatcccg
       61 tgccgggccg acacggcgtc caagaccgcg cgggcggacg cgatcacctc ggtgccgatc
      121 ccgtcgcccg ggatgacggc gatgcgctgc gggctcaccc ggtcgatcct aggcgggtcg
      181 ccacggacca ccgtcacagc tgatgcaaag gagtgcgagg cagtgtggac tcgtgcatgt
      241 cctgtcccgc cgccgcgtca tccagctggc cggcgtcgcc agcgcttcgg ccatggtggc
      301 ggcgtgctcc ggcaacgcca accccagcag gcaaccaggc cgcgcggaac acagggccac
      361 tcccacggcc aagccgcccg cgacgcccag tgcggccgat ggcgcctgcc cgaagacgac
      421 gccgcccgag accgccggac cgcatcccgc tgacgggtcg aacggcccca acgtcctcgg
      481 cgaggagggt gtcgtccgca gcgacatccg gtcgagcttc ggcgccgtgt ccggctgggc
      541 cgaaggtgtt cagacgacca tcgagttgac tgtgttggac gtgacaagag actgcgccaa
      601 gggcgagggc atggccgtct atgtctggca ctgcgaccgg gcaggcgatt actcgctcta
      661 cagcggggat gccgccgaca agaactacct ccgcggcgtt caggtggccg ggcaggacgg
      721 cagggtgtcg ttcacttcgg tgttccccgc gtgcgacccc gggcgctggc cgcacatcca
      781 cttcgaggtg ttcgattcgc tcgacgcggc agtcgcaggc gtaaacgccc gcctcacttc
      841 acagctggcg ttgcccgaag gtgcgtgcaa gaaggtgttc gactacgaca agggctactc
      901 ggccagcgtg cagaacatgt cgaacgtaac gccggagaaa gacagcgtgt tcggcgacgg
      961 ctgggatggc gagcttgcga cggtgaccgg cgatccgtcc agcgccatgt tggtcactct
     1021 gacgatcggc gtcggcgaga agccaagccc cccgccgccc tgacggatca cggccacggc
     1081 tcggccgcgt tatccccgta ttcgaactcg atgccgacgc ttaaccatta ccggcgtcgg
     1141 cgcgtttccg gacgccttat agtggggatc cggtcggaat caatccgatc gattatccgg
     1201 ccccctgccg acccacattg gagtgctttt gtcttggtct cgccgcgccc gaatcggctc
     1261 cgtcgccacc gtcgtggcgg cggtggtgct gcttgccgga gatgtcgccg acaccaccgc
     1321 gcagtgctcc acccagtgcc aggtcgccag cgccgcgcag accatcgcca agcctgaacc
     1381 ggcgcctcca caacccgcgg caatcggcgt cttcccgcag gcacaggccg acgatgtcaa
     1441 tcccgccgca ccggtctccg tcgccgcctt ctccggaagc atcgtcaacg tcgagatggt
     1501 caacgacaac ggcaccacgg tcccgggcgc gctgacaccc gacaagaagg gctggcaccc
     1561 gaccgaacaa ctcggctacg gccggaccta cacgatgacc gtcgtggccc gcggacccgg
     1621 cggcatgccg tcacgtcaga cgtccagctt caccactttg acgccggact acgaaaccgc
     1681 cgtcaacctc aacaccatcg ccggtgtttc gctcgtcgac ggcactacct acggcgtcgg
     1741 caccgtcgtc gtcgcgcact tcgacgaagc catcaccgac aaggtcgccg ccgagaagcg
     1801 cctgcatgtc accaccaacc cgcccgtcgc gggagtctgg aactgggtcg acgatcaaga
     1861 ggcccactgg cggccagaga agtactacgc accagggact gccgtcgacg tcaccgcgga
     1921 catctacggc gccgagctcg gcgacggcat gtacggccaa gacgaccaac acgtcgcctt
     1981 caagatcggc aacgcgcacg tctcgatcgc cgacgacacc accaagcagg tcagcgtgtt
     2041 cgacaacggc aagctggtcc gcaccatgcc gacctcgatg ggcatgggcg gcacccagac
     2101 catcggcggc accacgctgt cgttctggac cccgcctggc acctacaccg tgctggacaa
     2161 ggccaacccg gtgatcatgg actcctcgac cttcgggctg ccgacgggct cgcggctcgg
     2221 ctaccgcgag accatcccgt acgccacccg catcagcatg gacggcatct atcttcacca
     2281 gctgaacgcc accgtatggg cccagggcaa cacgaacacc tcgcacggct gtctgaacct
     2341 caacggcgag aacgccgcgt ggtttttcga cttctccgtc cccggcgacg tcgtcgaggt
     2401 ccaccacacc ggcgggcccg cgctgaccgt caagcagggc ggcgactgga ccgtgccgtg
     2461 gaaggaatgg cgtcagggca gcgcgctcaa cgcgtgacgg ccatgcctgg ctcctcgacg
     2521 gtgcgctgtg cacgcggcgc ggacgacgga ccgtccagcg gatccccagt cgtcggctcg
     2581 ggtgcgcccg cgcgtaccgc ctcgagctgc gcggcgaagg cgattccgag gaacagtgcg
     2641 accgacgaca ggttcgccca caacagcaac gcgaagatcc ccgtcaacgg cccgtaggtg
     2701 ctgccgaacg agccgctctt gacgacgtac agcgcgagca accccgtcag caacagccac
     2761 agcagaagcg acactccggc cccgaatgcc aaccacgagt accccggctg ccgtcgtcga
     2821 ggcgcgcgct ggatgacgac ggtgaacgac gccaacgcca gaagcactcc gagcggccaa
     2881 cttccgatcg tccacagcga ctggaacgtg tcgccccagc catatgccgc cgcaagcgcc
     2941 tcgccgagtg cgtcaccggc gatgatcacg aggaatccaa gctgaagcag cagtccggcc
     3001 gtcatcgcca tcagtagtgc gcggccgtac ttccgcagcg cgggccggtc gcgttcgatg
     3061 ccgtagatgc ggttcgcgcc ccgctcgatc tgccccatcg ccgtggtcag cgcgacaatg
     3121 gcggcgagca gacccagcca caacgcgaga tggccg
//
LOCUS       DASYLX010000097         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2857274,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000097 DASYLX010000000
VERSION     DASYLX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857274"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VGP27_02355"
     CDS             <1..843
                     /locus_tag="VGP27_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7850190.1"
                     /translation="YHSLQAVRSELAWMAALREQTDVVTPQLIPACDGSEVVTVVRNE
                     HPLNVDAVTYVAGCTAEERPQAVGFDQLGRITAAMHDHVEGWKTPSYFTRFSWNADTM
                     IGPDGRWGNWRHAPGLTETGEAMAGSAVTELRRRLAGFGCTPDRFGLIHADLRMANLM
                     IDPDVAAGPITVIDFDDCGWSWYLADLGAVVSFIEDTPAAEAMIAGWLAGYRESRELP
                     AEHLDMIPSFVMLRRLQLTAWVASHAHADSAIEFTEGFADGTARLAERYVKDRDWLRD
                     AVYP"
     gene            complement(834..1481)
                     /locus_tag="VGP27_02360"
     CDS             complement(834..1481)
                     /locus_tag="VGP27_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7850191.1"
                     /translation="MQQVLSDLWQTRTDSPFPGLTTHAFLWTGRNTLFYSTATDADFA
                     ELDELGGVCDQYLSHRDEAGPMLAKIAGHFDSSLHAPVTELLEIGRHAHVDVPIGSRH
                     IDVNGIEVIPTPGHSPGSTCYLVNGAEGRYLFTGDTLVFGDSGNWWAGYIPGIGDAEA
                     LAASLRLLRSLTPDVVVSSAFQGDSAVHRIQPGCWKDCVDEALDGLAADSAGRYG"
     gene            complement(1522..2610)
                     /locus_tag="VGP27_02365"
     CDS             complement(1522..2610)
                     /locus_tag="VGP27_02365"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7850192.1"
                     /translation="MRLPVMPPVSPMLSKSVPTIPPDASYEPKWDGFRSILFRDGDEV
                     ELDSRNERPMTRYFPELVAAARAELPDRCVIDGEIVIAAGGRLDFEALQLRLHPAASR
                     VRLLAGQTPASFIAFDLLALGDDDYTQRPFTERRAALIEALASAAHARSRRGSSPSIH
                     VTPATTDMTTAQRWFSDFEGAGLDGIVAKPLTIPYQPDKRIMFKIKHQRTADCVVAGY
                     RLHKSGADAIGSLLLGLFKDDGTLASVGVIGAFPMAKRRQLLTELQPLVTTFEQHPWN
                     WAAHFAGERTPRKNEGSRWNAGKDLSFVPLRPERVVEVRYDHMEGERFRHTAQFNRWR
                     PDREARSCTYDQLERPVTFALDDIVPGL"
     gene            2670..3722
                     /locus_tag="VGP27_02370"
     CDS             2670..3722
                     /locus_tag="VGP27_02370"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7850193.1"
                     /translation="MESVDLPVQPPIDPMLAKAQAKVPDEAGVWSYEPKWDGFRALVF
                     RDGDDVVLQSRNGKDLGRYFPELLDALRDEVAPRCVLDGEIVVPREIGGRIRLDWESL
                     SQRIHPAASRITMLSEQTPAHFIGFDALATGDASLLKQPFRTRREALCDAVKETQWCH
                     VTRTTEDPELGVQWLTTFEGAGLDGVIAKRLDGPYLPGKREMVKVKHARDADCVAIGY
                     RIHKSGEGVGSILLGLYRDDGELQMVGGAASFTAKDRLKLLADLEPLREGDGMREGDP
                     SRWNSAADKRWVPVRPEKVCEVAYDQMEGNTEHGQRFRHAVKFVRWRPDRDPDSCTFD
                     QLDVPVNYDLYDVLES"
     gene            3724..>4544
                     /gene="ligD"
                     /locus_tag="VGP27_02375"
     CDS             3724..>4544
                     /gene="ligD"
                     /locus_tag="VGP27_02375"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV7850194.1"
                     /translation="MATPAEELDVDGVKVRFTNPDKVYFPKLGRNGTKRKLVEYYLAV
                     ATGPMLTALRDRPTHLQRFPDGIDGEEIYQKRVPQKHPDYLETCVVTFPSGRTADALK
                     VTHPSAIVWAAQMGTITLHPWQVRCPDTEHPDELRIDLDPQPGTGFREAGAIAVDVLK
                     PLLDELGLIGFPKTSGGRGVHVFLRIKPDWDFIAVRRAGIALAREVERRAPDAVTTSW
                     WKEERGRRIFIDYNQNARDRTFASAYSVRKTPIATVSMPVTWDELREADPDDYTIA"
BASE COUNT          802 a         1440 c         1552 g          750 t
ORIGIN      
        1 taccactcac tgcaggcagt ccgttcagag ctggcctgga tggccgcgct acgcgagcag
       61 accgatgtgg tgacgccgca gctgatcccg gcctgcgatg gcagtgaggt cgtcaccgtc
      121 gtccgcaacg agcatccgct caacgtcgac gccgtgacct acgtcgccgg ttgcaccgcg
      181 gaggaaagac ctcaggcggt cggtttcgac cagctcggac gaatcaccgc cgccatgcac
      241 gaccacgttg agggctggaa gacgccgagc tacttcaccc gcttctcctg gaatgccgac
      301 accatgatcg gcccggacgg ccgatggggg aactggcggc atgcgcccgg gctgaccgaa
      361 accggcgagg cgatggccgg aagcgctgtg acggagctgc gacgcagatt ggccggcttc
      421 ggttgtacgc ctgaccgttt cggtttgata cacgctgatc tgcgaatggc gaatctgatg
      481 atcgatccgg acgtggccgc agggcccatc accgtcatcg acttcgacga ctgcggatgg
      541 tcttggtatc tggccgatct cggtgccgtg gtgtcgttca tcgaggacac tcccgcggcc
      601 gaggcgatga tcgcgggatg gctggcggga taccgcgaaa gtcgggaact gcccgccgag
      661 catctcgaca tgatcccctc gttcgtcatg ctgcgacgcc tgcagctgac ggcatgggtg
      721 gcgtcacacg cccacgccga cagcgccatc gagttcaccg aaggcttcgc cgacgggacg
      781 gcgcggctgg ccgagcgcta tgtcaaggac agggactggc tgcgcgacgc cgtctacccg
      841 taacgacccg ccgaatccgc cgccagaccg tccagcgcct cgtcgacaca gtccttccag
      901 catccgggct ggatgcggtg caccgccgag tcaccctgaa aggcgctgga aaccaccaca
      961 tccggggtca gactacgcag cagccgcagg ctcgcggcca gcgcctcggc gtcgccgatg
     1021 ccggggatgt agccggccca ccagttcccg gaatcgccga acaccagggt gtcgccggtg
     1081 aaaaggtaac ggccctcggc gccgttcacc agatagcagg tgctgcccgg tgagtggccg
     1141 ggcgtcggga tgacctcgat cccgttgacg tcgatatggc ggctgccgat cggcacgtcg
     1201 acatgagcgt gccggccgat ctccagcagc tcggtgacgg gcgcgtgcaa ggacgagtcg
     1261 aagtggccgg cgatcttcgc gagcatcggg ccggcttcgt cgcggtgcga cagatattgg
     1321 tcgcagacgc cgccgagctc atcgagttcg gcgaagtcgg catccgttgc agtggaatag
     1381 aacagggtgt tgcggccggt ccacaggaat gcgtgagtgg tcagtcccgg aaacggtgaa
     1441 tcggtgcggg tctgccatag atcactcaga acttgttgca tacgtccacc ctgcaacctg
     1501 aaccgaagtt gaggtcaagc gtcacaaacc ggggacgata tcgtcgaggg cgaaggtgac
     1561 cggccgctcg agttggtcat aggtgcatga gcgtgcttcg cggtcgggcc gccaacggtt
     1621 gaattgtgcg gtgtggcgga acctttcgcc ttccatgtgg tcgtaacgga cctcgacgac
     1681 ccgctcgggc cgcagcggca cgaacgaaag atcctttccc gcgttccagc gggaaccttc
     1741 gttcttgcgt ggggtgcgtt ccccggcgaa atgggcggcc cagttccagg ggtgctgttc
     1801 gaaggtggtg acgagcggct gcagttcggt gagcagttgc cgtcgcttcg ccatcggaaa
     1861 ggcgccgatg acgccgacgg acgcgagtgt gccgtcgtcc ttgaagagcc cgagcagcag
     1921 cgagccgatc gcgtccgcac cggacttgtg cagccggtaa cccgcgacga cgcagtcggc
     1981 ggtgcgctgg tgcttgatct tgaacatgat gcgtttgtcc ggctggtagg ggatggtcag
     2041 cggcttggcg acgatcccgt ccagacccgc gccctcgaag tcggagaacc agcgctgcgc
     2101 cgtcgtcatg tcggtggtgg cgggagtgac gtggatcgac gggctcgagc ctctcctgct
     2161 ccttgcgtgt gcagcgctgg caagtgcttc gatgagggcg gcgcggcgct cggtgaacgg
     2221 gcgctgtgtg tagtcgtcgt cgccgagggc gagcagatcg aacgcgatga acgatgccgg
     2281 tgtctgcccg gcgagcagcc gcacccgtga tgcggcggga tgcagccgga gctgaagcgc
     2341 ctcgaaatcc agcctgccgc cggcggcgat gacgatctcg ccgtcgatga cgcaccggtc
     2401 gggcaactcc gctcgagccg cggccacgag ttcggggaag tagcgggtca tcgggcgctc
     2461 gttgcggctg tccagctcga cctcgtcacc gtcgcggaag agaatcgacc ggaacccgtc
     2521 ccacttgggc tcataggagg cgtcgggcgg gatcgtcggc accgatttcg acagcatcgg
     2581 cgacaccggc ggcatgacgg gcaggcgcat cggcccattc tcgctcggct gcccgatgac
     2641 attgcccggt gtcggctgtc tacaggagta tggaaagtgt ggacctcccc gtgcagccac
     2701 cgatcgatcc catgctcgcc aaggcccagg cgaaggtgcc cgacgaggcg ggcgtgtggt
     2761 cctacgaacc gaaatgggac ggcttccggg cgctggtgtt ccgcgacggc gacgacgtcg
     2821 tgctgcagtc gcgcaacggc aaggacctcg gccggtactt ccccgagctg ctcgacgcac
     2881 tgcgcgacga ggtggcgcca cgctgcgtgc tcgacggcga gatcgtcgtc ccgcgtgaga
     2941 tcggtggccg cattcgcctc gactgggagt cgctcagtca gcgcatccat ccggccgcca
     3001 gccgcatcac gatgctttcc gagcagaccc cagcgcattt catcggtttc gatgcgctgg
     3061 ccaccggtga cgcgtcgctg ctcaagcagc cgttccggac gcgacgggaa gcgctctgcg
     3121 acgcggtcaa ggaaacacag tggtgtcacg tcacccgcac gaccgaagac cctgagctgg
     3181 gcgtgcagtg gctgacgacg ttcgagggcg cgggcctcga cggggtgatc gctaaacgtc
     3241 tcgacggccc gtacctgccc ggcaagcgcg aaatggtgaa ggtcaagcac gcccgcgacg
     3301 ccgattgcgt ggccatcggc taccgcatac acaagagcgg ggagggtgtc ggttcgatcc
     3361 tgcttgggct ctaccgcgac gacggtgaac tccagatggt cggtggcgcg gcatcattca
     3421 ccgcgaagga ccggttgaag ctgctcgcag atctggaacc gctgcgagaa ggcgacggaa
     3481 tgcgcgaagg cgaccccagc cgatggaact cggcggccga caagcgctgg gtcccggtgc
     3541 ggccggaaaa ggtatgcgag gtggcctacg accagatgga aggcaatacc gagcacgggc
     3601 agcgcttccg gcatgccgtc aagttcgtcc gctggcggcc cgaccgtgat ccggacagct
     3661 gcactttcga tcagctcgac gtgccggtga actacgatct ctacgacgtg ttggagtctt
     3721 gaaatggcaa cgcctgcaga agaactcgat gtagacggtg tcaaggttcg cttcaccaac
     3781 ccggacaagg tgtacttccc gaagctgggc cgcaacggaa ccaagcgcaa gctggtcgag
     3841 tactacctgg ccgtcgcgac cgggccgatg ctgaccgcgc tgcgggaccg gcccacccat
     3901 ctgcagcggt tccccgacgg catcgacggc gaggagatct atcagaagcg ggtgccgcag
     3961 aaacaccccg actatctgga gacctgcgtc gtcactttcc cgtccggccg aacggccgat
     4021 gcgctcaagg tgacgcaccc gtcggcgatc gtgtgggccg cgcagatggg caccatcact
     4081 ctgcacccgt ggcaggtgcg ctgcccggac accgaacatc ccgacgaact gcgcatcgac
     4141 ctcgacccgc agccgggcac cggattcagg gaggccggcg cgatcgcggt cgacgtgctc
     4201 aagcccttgc tggacgaact cggtctgatc ggctttccga agacgtccgg cggtcgcggt
     4261 gtgcatgtgt tcctgcgcat caagcccgac tgggacttca tcgcggttcg tcgtgccggt
     4321 atcgcgctgg cccgtgaggt ggagcggcgc gcccccgacg ccgtcacgac gtcgtggtgg
     4381 aaggaagagc ggggccggcg gatattcatc gactacaacc agaacgcgcg cgatcgcacg
     4441 ttcgcatcgg cgtactcggt gcgcaagacg ccgatcgcga cggtgtcgat gccggtgaca
     4501 tgggatgaac tgcgcgaggc cgaccccgac gactacacca tcgc
//
LOCUS       DASYLX010000098        11081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3844413,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000098 DASYLX010000000
VERSION     DASYLX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844413"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGP27_02380"
     CDS             <1..229
                     /locus_tag="VGP27_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dolichyl-phosphate-mannose--protein
                     mannosyltransferase"
                     /protein_id="HEV7850195.1"
                     /translation="AATMSPFLVMMIAMICGDILHQPNQNPERRTLGLIAVSCYVALV
                     ITNFAWMYPILTGLPISQSTWNLEIWLPSWR"
     gene            344..1204
                     /locus_tag="VGP27_02385"
     CDS             344..1204
                     /locus_tag="VGP27_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850196.1"
                     /translation="MRDVFVGSDALARGGMTRARLRWNYRTIFPDIYLPKQAVPSLRQ
                     RTHGAWLWSGRNGVITGRAAAAMHGALWVAVDSPIEMIWRCGRPPPGIEVRNERIDAA
                     DIVDIGGIAVTTPERTAFDLARHLRRDPAVAHLDALAQATRIAPPEVSMLADRYPRAR
                     GLSRARIALSLMDGGSQSPQETRLRLILIDDGLPPPRTQIMVSDGINRAFIDMGYEEP
                     MVGLDYDGKQHLTDRDRYVHDIGRAELIDREGWVDIHVVAEHSRRYILHRVYQAFARR
                     GCAPRLRTGS"
     gene            complement(1284..2489)
                     /gene="arcA"
                     /locus_tag="VGP27_02390"
     CDS             complement(1284..2489)
                     /gene="arcA"
                     /locus_tag="VGP27_02390"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV7850197.1"
                     /translation="MSGNALGVNSEVGTLRVVILHRPGAELQRLTPRNNDRLLFDGLP
                     WVARAQQEHDAFADLLRSRGVEVLLLSDLLTEALASGAARMHGISAAVDSRRLGLPLA
                     QELSAYLRTLNADDLAHVLTAGMTFNELPFGENEFSLVRRMHHGADFVIDPLPNLLFT
                     RDSSFWIGARVAITSLALPARVRETSLTDLIYAHHPRFLGVRRAYESRSAPVEGGDVL
                     LLAPGVVAVGVGERTTPAGAEALARSLFDDDLAHTVLAVPIAQERAQMHLDTVCTMVD
                     TDAVVMYPNIVDSLSAFTISQKGSGVTISRAAPFVDAAADAMGIGKLRVIDTGLDPVT
                     AEREQWDDGNNTLAVAPGVVVAYERNVETNARLADSGIEVLPIAASELGTGRGGPRCM
                     SCPAARDPL"
     gene            2554..2985
                     /gene="soxR"
                     /locus_tag="VGP27_02395"
     CDS             2554..2985
                     /gene="soxR"
                     /locus_tag="VGP27_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEV7850198.1"
                     /translation="MDLHELTPGEMADRSGVAVSALHFYERHGLIASRRTSGNQRRYA
                     RETLRRVAFIRMSQRLGIPLARIREALATLPIDRVPTSKDWARLSAGWRQDLDDRIVH
                     LQRLRDNLTGCIGCGCLSLKTCALTNPGDVLADRGPGAVRL"
     gene            3019..3615
                     /locus_tag="VGP27_02400"
     CDS             3019..3615
                     /locus_tag="VGP27_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEV7850199.1"
                     /translation="MEVALQSSLFDPAERQHLGSGAWVEVRSGWLTDADSLFDELVEA
                     IPWRAERRQMYDRVLDVPRLVSFHNLIDEPAPHPMLKQMRRRLNDTYGGELGEPFTTA
                     GLCLYRNGDDSVAWHGDTVGRSSTEDTMVAIVCLGATRVFALRPRGGGKSLRLQHHHG
                     DLLVMGGSCQRTWEHAIPKTVKPVGPRISIQFRTHDVR"
     gene            complement(3619..4278)
                     /locus_tag="VGP27_02405"
     CDS             complement(3619..4278)
                     /locus_tag="VGP27_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HEV7850200.1"
                     /translation="MLNLRAVFAVACAGALAACGSAEPDLSHANIANIKNLRSDFPPP
                     FKVSDIAPTAIDPKLVEPQKPPAGMKFEPADCAQFASQKLLPSGVKGNMSAITAVGDG
                     NRFIVIAVETSEAVPANEPTSNCRKVGYSGPALRGFVEVVDAPKIDGVQTQGAHRVVQ
                     TMVDGKARTGELYNYVAKFGTFVVIVTANPLVIPGKPVAPVDTKKAGELLSAGVSAVK
                     A"
     gene            complement(4307..4981)
                     /locus_tag="VGP27_02410"
     CDS             complement(4307..4981)
                     /locus_tag="VGP27_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HEV7850201.1"
                     /translation="MRRIALVEAFAVACVLAACGQPGTSTQTSTTAPSSANLQVNPAR
                     VSRTRGELPAGYEVADVAGPASPAAFWGFRPGWVTEPPQCGVLAGPVTDDATTQGWSG
                     SGPGGIVHVAVTGPSAAPVSVDPSVLAECGQWTVTSGNTTGTVDLVEAPAIEGATTVG
                     MNTVAKTVVEGGTETTSAAYTFSAYLGDYLTFVTVVTDPGSPNPALGQEFAADLLVET
                     VSALRG"
     gene            5088..5420
                     /locus_tag="VGP27_02415"
     CDS             5088..5420
                     /locus_tag="VGP27_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850202.1"
                     /translation="MRAALAMAGGIIAAGVGLAPGITAAAEPIAPGGAYATIGQLEAS
                     GYDVNIDRVGSAPLDQCIVTSVRNPQDITRTIRVDNGRKGDKRDFDYVTIVVRRTITV
                     SLNCDART"
     gene            complement(5417..6946)
                     /locus_tag="VGP27_02420"
     CDS             complement(5417..6946)
                     /locus_tag="VGP27_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HEV7850203.1"
                     /translation="MKSIARSLIVVLVALVAVPALVVTATITTAVQLLAVTGLYMGGT
                     EHPLGPPDSPGFVEEYMNLADANYIGPATGETPSTRVAVVYPAQFFPVFGSTTFDESV
                     DAGRDNLNSCIRSAPCNGSDPIEMGPEDTFVVFGYSQSAVVASLVKRDLIEHPTTEVG
                     TAPDSAEFVLLSNPMRPNGGILGRGFEGLTIPILGITFYGATPTNSCQKPGDPCYPTT
                     DIAYQYDLLGGDAPAVPWNVLAWANSAAAYYYLHGDAPNQSLDGAVYQGTTGDTDYYL
                     LTSDVLPLLMPLEQVGVPKPILLVANAPLKVLIEAGYYREPGPGEKVPFQLLPEKDPI
                     TLVVNLAKSVPVGIDDGLDEAGVGRALGTDPVTSPYGVGGRELPPEPDENLTANAALE
                     GSPAPEGAAAPQPDPTPVQPLAGGGTEKTIPDLHLSKPGNDLRQLGNEDISTPDTPPA
                     TTPRPLQIVRDSLSFEPKPPAATRPSGDGPLKRIVNALTGQRPDAAADSDAGDESKDA
                     A"
     gene            7279..7965
                     /locus_tag="VGP27_02425"
     CDS             7279..7965
                     /locus_tag="VGP27_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEV7850204.1"
                     /translation="MTKVRAGGIAIMAVAVGLALAGCGSNDKKTEATTSSSTSSSSKA
                     APSQEAVKPNATIPDYIKQNGITESPVKRGDPGSPEINLPVPPGWEDMGDGTPPWAWS
                     GMKFTGDPAMAASPPTILALLSKLQGNVDPAKILEFAPGEMKNLPGYEGDGVGSASTL
                     GGFDAWQVGGMYTKDGVKHAVAQKTVVIPGADGLFVLQLNADGTEDQMGALMGATNVI
                     DEQTTITPAP"
     gene            complement(8073..8891)
                     /locus_tag="VGP27_02430"
     CDS             complement(8073..8891)
                     /locus_tag="VGP27_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate 5-dehydrogenase"
                     /protein_id="HEV7850205.1"
                     /translation="MRPPLSKDTRVCISLAGRPSNIGTRFHNYLYDLLGLDFVYKAFT
                     TTDIVGAIGGVRALGIRGCSVSMPFKQDVIPLVDRVEQSAQAINAVNTIVNDGRHLTA
                     SNTDYIAVQRLIEEHGLQRSQSVLIHGSGGMAGAVGAAFRDSGFQKGLIVARNTEAGA
                     ALARQLGYEYAAEVGSRTADVIVNVTPVGMAGGPEERLSPFDTSAIAKADTVFDVVAL
                     PSETPLIAAARAAGVNVITGAEVIALQAAEQFERYTGVRPTAEQVADASAVSRA"
     gene            complement(8891..9889)
                     /locus_tag="VGP27_02435"
     CDS             complement(8891..9889)
                     /locus_tag="VGP27_02435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7850206.1"
                     /translation="MAGLTAVSADELAALEIFKGCRPDALVSLAAQLRPLNAPAGRVL
                     MRQGERAESFLLIGAGKAEITHTGNDGVPIAVELSPPLIVGEIALLREAPRTATVVAT
                     EPLTGWVGGHGAFAAMLDVPGMMDKLVRTARQRLAAFITPIPIQMRDDSWLYLRPVLP
                     GDSERSSEGTIEFSSETLYRRFQTPRTPSQSLMTYLFEVDYKDHFVWVMTEGGEGPVV
                     ADARFVRDETDRTLAEVAFIVGDDYQGRGVGSFLMAALAVAARSDGVRRFSARVLSDN
                     LAMRKVLDRFGAQWQREDLGVVTTVIDVPTLRDLGMSKELYREIHGVARQVLGAVG"
     gene            complement(9912..>11081)
                     /locus_tag="VGP27_02440"
     CDS             complement(9912..>11081)
                     /locus_tag="VGP27_02440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850207.1"
                     /translation="GTWVVSGAISVLLNVGGGTGIVAATAFGVVLGGSAATGIGLLLT
                     MNLIRPLLAAATHASERLVTAPGVFARLILMWLLCSALPCLVIVALVVVRSNGWLIPK
                     TASVEIAALVVASAAVVLGLPTMILTSRSIADPIGEVVDAMAEVERGRFDTSVDVFER
                     SEIGRLQSGFNQMVAGLAERERVRDLFGRHVGTNVALRAIEEGAAMSGDVQEAAILFI
                     DLTGSTALAASHPPQRVAEVLNEFFHIVVNAVDARDGMINKFQGDAALAVFGAPWRSN
                     GAASSALATARALGEQLRELPLVDFGIGVSAGPVFAGNIGAENRYEYTVIGDPVNEAA
                     RLADLAKTSDRRILCSAAAIERADTAERTHWAAKGSEVLRGRSEATEISAPDGHR"
BASE COUNT         1972 a         3705 c         3554 g         1850 t
ORIGIN      
        1 tgcggcgacc atgtcgccgt tcctggtcat gatgatcgcg atgatctgcg gggacattct
       61 gcatcagccg aaccagaacc ccgaacgaag aacgctcggc ctcattgccg tcagctgcta
      121 tgtggccttg gtgatcacga acttcgcgtg gatgtacccg atcctcacgg gcttgccgat
      181 ctcgcagtcg acctggaatc tggagatctg gcttcccagc tggcggtagc ccggcggggc
      241 tgaagattgc acgtagggct gttgcgaggc gttatccaca gccctgtgtg caatctttcc
      301 gagtgccgct caatttgtcg ggtcgattcg acagcatcgc ggcatgcgag acgtgttcgt
      361 cgggagtgat gcgttggctc gcggcggcat gacgcgcgca cgcctgcgct ggaattatcg
      421 aacgatcttc ccggacatct acttgccgaa gcaagcagtg ccgtcgctca gacagcggac
      481 tcacggcgct tggctgtggt cgggccgaaa cggtgtcatc accggccgcg cggcagccgc
      541 gatgcacggc gccctgtggg tagccgtcga ctcaccgatc gaaatgatct ggcgatgtgg
      601 tcggccacca ccaggcatcg aggtgcgcaa tgaaagaatc gacgcggccg acatcgtcga
      661 catcggcggc attgccgtaa cgactcccga acgcacagcg ttcgacctgg cacgccacct
      721 tcggcgcgat cctgcggtgg ctcatctcga tgcgcttgca caggcgaccc ggatcgcccc
      781 acccgaagtc agcatgctcg ccgaccgcta tccgcgagct cgtggcctat cccgcgctcg
      841 aatcgcgttg tcgttgatgg acggtggttc gcaatcgcct caggaaacac ggctgagact
      901 gattctcatc gatgatgggc taccaccgcc acgcacccag atcatggtga gcgacgggat
      961 taatcgggcg ttcatcgaca tgggctacga ggagccgatg gtcggcctcg attacgacgg
     1021 caagcaacac ctgaccgatc gcgatcgcta cgtgcacgac attggacggg ccgagctgat
     1081 tgaccgagaa ggctgggtcg acatccacgt cgttgccgaa cacagcaggc gatacatcct
     1141 gcatcgcgtt taccaagcct tcgcccgccg cggctgtgcc ccaagattgc gcacagggtc
     1201 gtgattctcg atggatcgca gccttcactg caattttgcg ggcggcgatt acagcgggtc
     1261 acggcgatta cagcgggtgg cgattacagc gggtcgcgcg ctgcgggaca ggacatacag
     1321 cgcgggccgc cgcggccggt ccccagctct gaggcagcga tcggcaacac ttcgatgccc
     1381 gaatcggcga ggcgcgcatt cgtttcgacg ttgcgctcgt aggccaccac gacaccgggc
     1441 gccaccgcca gcgtgttgtt gccgtcgtcc cactgctcgc gctcggccgt gacgggatcc
     1501 aggcccgtgt cgatcacgcg cagcttgccg atccccatcg catccgccgc ggcgtcgacg
     1561 aacggcgccg ctctggagat cgtcacaccg ctgcccttct ggctgatcgt gaacgccgac
     1621 aacgagtcga cgatgttcgg atacatcact acggcatccg tgtcgaccat cgtgcacacc
     1681 gtgtcgaggt gcatttgcgc gcgctcctga gcgatgggca cggcgagcac cgtgtgcgca
     1741 agatcatcgt cgaacaggct ccgcgccaac gcctcggcgc ctgcgggcgt ggtccgctcc
     1801 cccaccccca ccgcgacgac accgggcgcc agcagcagaa cgtctccacc ttccaccggc
     1861 gccgaccgtg attcgtacgc ccgtcgcacg ccgaggaagc gcgggtggtg ggcgtagatc
     1921 aggtcggtga gcgacgtctc acgcactcgc gcaggcagcg ccagcgaagt gatggccact
     1981 cgcgcaccga tccagaacga cgagtcgcgg gtgaacaaga ggttgggcag cgggtcgatg
     2041 acgaagtccg cgccgtgatg catccgtcgc accaacgaga attcgttctc cccgaacggc
     2101 agctcgttga acgtcatgcc tgccgttagg acgtgagcca gatcgtcggc attcagcgtg
     2161 cgcaggtagg ccgaaagttc ttgcgccagg ggcagcccga gccgacgcga gtcgacggcg
     2221 gccgagatac cgtgcatccg ggccgcaccg ctggccagcg cctccgtcag cagatccgac
     2281 agcagcagca cctcgacgcc gcgcgaccgc agaaggtcgg cgaaggcgtc gtgctcctgt
     2341 tgggcgcggg ccacccatgg caacccgtcg aacaacagcc tgtcgttgtt gcgcggcgtc
     2401 agccgctgca attcggcgcc ggggcggtgc aggatgacca cccgcaacgt cccgacctct
     2461 gagttcaccc ccagcgcgtt cccagacacg tatgcaccgt atcgccttta ggcacctgaa
     2521 ctgttgttta gccacctcaa ctattgttta ggtatggacc tccacgaact gacgcctggc
     2581 gagatggccg atcgcagtgg cgtggccgtc tcggcgctgc acttctacga acgccatggc
     2641 ctgatcgcca gccgccgaac gtcgggcaac cagcgccggt acgcacgcga gacgctgcgg
     2701 cgtgtcgcgt tcatccgcat gtcccaacgg ctcggcattc ccctcgcccg catccgggag
     2761 gcgctggcga cgctgcccat cgaccgcgtc ccgaccagca aggactgggc acggctgtcc
     2821 gcaggctggc gccaggacct cgatgaccgg atcgtgcacc tgcaacggct tcgcgacaac
     2881 ctcaccggct gcatcggctg cggatgcctc agcctgaaga catgcgcact gaccaacccc
     2941 ggcgacgtcc ttgccgacag aggacccggc gcggtgcggc tctgacggct cgaacgggtg
     3001 tgcgataaac tggcgcgtat ggaggtggct ctacaaagct ctctcttcga ccccgccgaa
     3061 cggcagcacc tcggcagcgg cgcgtgggtc gaggtgcgct cgggctggct gaccgacgcg
     3121 gactcgctgt tcgacgagct cgtggaggcc atcccgtggc gtgccgagcg cagacagatg
     3181 tatgaccgtg tgctcgatgt gccccgcctg gtgagctttc acaatctgat cgacgaaccg
     3241 gcgccgcacc cgatgctcaa gcagatgcgc aggcggctca acgacaccta cggcggcgaa
     3301 ctcggcgaac ccttcaccac ggcgggccta tgcctctacc gcaacggcga cgacagcgtg
     3361 gcctggcacg gcgacaccgt cggccgtagc agcaccgagg acaccatggt ggcgatcgta
     3421 tgtctgggcg ctacaagggt tttcgcgcta cggccgcgcg gtggcggcaa gtcgctacgg
     3481 ctgcagcacc accacggcga tctcctggtg atgggcggtt cgtgccagcg aacgtgggag
     3541 cacgcaatac cgaagacggt gaagccggtc ggcccgcgga tcagtatcca gttccggacg
     3601 cacgacgtcc gttgagcctc aggctttcac cgcggacact cccgcgctga gcaactcgcc
     3661 ggctttcttg gtgtcgacgg gcgccacggg cttgccgggg atcacgagcg ggttggcggt
     3721 gacgatgacg acgaaggtgc cgaatttcgc gacgtagttg tacaactcgc cggtgcgggc
     3781 cttgccgtcg accatggtct gaacgactcg atgcgcgccc tgcgtctgca cgccgtcgat
     3841 cttcggtgca tcgaccacct cgacgaaccc gcgcaacgcc ggaccgctgt atccaacctt
     3901 ccggcagttc gacgtcggct cgttggcagg cacagcctcc gacgtctcca ccgcgatcac
     3961 gatgaaccgg ttaccgtcgc ccaccgccgt gatcgccgac atgttgccct tcaccccgct
     4021 ggggagaagc ttttgactgg cgaactgtgc gcaatcggcg ggctcgaatt tcattcccgc
     4081 aggcggcttt tgcggctcga ccagctttgg gtcgatggcc gtcggggcga tgtcgctgac
     4141 cttgaacggc gggggaaaat ccgaccggag gttcttgatg ttggcgatgt tggcgtgcga
     4201 cagatcaggc tccgcagagc cgcatgcggc aagagcaccc gcgcatgcga cggcgaacac
     4261 cgccctcagg ttcaacatcg ccgccaatgt acccgcggac gcggcttcag ccgcgtaacg
     4321 ccgacactgt ttcgaccaat agatcggcgg cgaattcttg gccgagtgca gggttcggcg
     4381 atcccggatc cgtgaccacg gtgacgaacg tcaggtagtc accgagatag gcgctgaagg
     4441 tgtaggcggc cgaggtcgtc tccgtgccgc cctcgacgac cgtcttggcg acggtgttca
     4501 tgccgacggt ggtggcgccc tcgatggccg gggcctcgac gaggtcgacg gtgccggtgg
     4561 tgtttccgga cgtcaccgtc cactgcccgc attcggccaa taccgacggg tcgacggaga
     4621 cgggcgccgc cgacgggcct gtcaccgcga cgtgcacgat tccgccggga ccagaccccg
     4681 accagccttg ggtggtcgcg tcatcggtga ccggcccggc gagcactccg cactgaggcg
     4741 gttcggtgac ccaaccgggg cggaaacccc agaaagcggc gggcgaagcg ggcccggcga
     4801 catctgcgac ctcatatccg gcgggcagct cgccgcgtgt ccggctgacc ctggcggggt
     4861 tcacctggag gtttgctgaa gacggcgcag tcgtcgacgt ctgagtcgac gtgccgggct
     4921 gaccgcacgc cgcgagcacg cacgccaccg cgaaggcctc aacgagcgcg atccggcgca
     4981 ccgcccgttg ataccacagg taaggctttg aaccggctgg caacactttg cgtgtctata
     5041 gcgatacaac agtgggccgg gcctaagaga agtgcggaga aactgtcatg cgcgcagcat
     5101 tggctatggc agggggaatc attgcggcgg gcgtggggct cgcgccgggg attacggcgg
     5161 cggccgaacc gattgcaccg ggtggtgctt acgcgacgat cgggcagctg gaggcttccg
     5221 gctacgacgt caacatcgac cgggtgggca gtgccccact cgaccagtgc atcgtcacca
     5281 gcgttcggaa tccgcaagac atcacccgga ccatccgggt ggacaacggc cgcaaaggcg
     5341 acaagcggga cttcgactac gtgacaattg tggtgcgacg gacgatcacc gtctcgctga
     5401 actgcgacgc gcgcacctaa gcggcgtctt tcgattcgtc gcctgcgtcg gagtcggcag
     5461 cggcatcggg ccgctggccg gtcagggcgt tgacgatccg cttcagcggc ccatcaccac
     5521 tgggccgggt cgctgccggt ggcttcggtt cgaagctcag cgagtcgcgc acgatctgca
     5581 gcggccgtgg cgtagtagcc ggaggtgtgt caggggtgga tatgtcttcg ttgcctagct
     5641 ggcgcagatc gttaccaggc ttggacagat gtagatcggg gatggtcttc tcagtcccgc
     5701 caccagccaa cggctgcacg ggcgttgggt ctggctgggg cgcagcggcg ccctcgggcg
     5761 ccggcgaccc ttcgagcgca gcattcgctg tcaggttttc gtccggttca ggcggcagct
     5821 ctcggccacc gacaccgtag ggactcgtaa ccgggtcggt tccgagtgcc cgcccgactc
     5881 cggcttcatc caggccgtcg tcgatgccca ccgggaccga cttggcgagg ttgacgacga
     5941 gggtgatcgg gtctttttcg ggcagcaact ggaatggcac cttctcgccc gggccgggtt
     6001 cgcggtagta ccccgcctcg atcagtacct ttagcggcgc gttggcgacc aacaagatcg
     6061 gcttggggac gccgacctgc tccaatggca tgagcagcgg tagaacgtcc gacgtgagca
     6121 ggtagtagtc ggtgtcgccg gtcgtgccct gataaacggc gccgtcgagc gactggttcg
     6181 gagcgtcgcc gtgcaggtag tagtacgcgg ctgccgaatt cgcccatgcc aagacgttcc
     6241 agggcaccgc gggggcatcg ccgccgagca ggtcgtattg gtaggcgatg tcagtcgtcg
     6301 ggtagcaagg atccccgggc ttctgacagg agttcgtcgg agtggctccg tagaaggtga
     6361 tgccaagaat cggaatggtc agaccttcga aaccgcgtcc gaggatgcca ccgttgggcc
     6421 gcatggggtt tgagagcagg acgaactcgg cgctatccgg cgccgtgccg acctccgtcg
     6481 tggggtgctc aatgaggtcc cgcttgacga gcgaagccac cacggcgctc tgcgagtagc
     6541 cgaagacaac gaacgtgtcc tccggaccca tctctatagg gtcgctaccg ttgcagggcg
     6601 cgcttcggat acagctattg aggttgtcac gcccggcgtc caccgattca tcgaatgtcg
     6661 tcgacccgaa cacgggaaaa aactgcgcgg gatagaccac tgcaacgcgg gtactgggag
     6721 tctcgccggt agcggggccg atgtagttgg cgtcagcaag attcatgtac tcctcgacga
     6781 agccgggcga atccggcggg cccaacggat gctcagtgcc tcccatgtac aacccggtga
     6841 cagccagtag ctgaaccgcg gtggtgatcg tcgctgtcac caccagcgcg ggcacggcga
     6901 ccagcgctac caacacaacg atcagcgaac gcgcgatcga tttcataccg agaccttccc
     6961 ccgccacccc cgaagctcgc gccgccttcc gtcagcgacg acacagcaag accggtgtgg
     7021 ccctccgcta cgcagtacag gcgcatcttg cactaccggc cgccaagatc gtagcgaaac
     7081 ggtaacggtt ttctgtggcg acgctggcaa agctctagtc atcgaccaat gggtcggcat
     7141 tatcagtcgc gggcaaagcg ctttgacgtg ctcctcgagc cgattctgcg acgaatgatc
     7201 ttcgcaacgg ttttccgtcc acaaatcgtt tgccgaccgt aggctcatgg cgcagattcc
     7261 accgacacgg gagactccat gacaaaagtt agggccggcg gcatcgccat catggccgtc
     7321 gcagtcggcc tggccctggc gggttgcggt tcgaacgaca agaagaccga ggccaccact
     7381 tcaagctcga ccagctcgtc cagcaaggcc gccccgtcgc aggaggccgt caagcccaat
     7441 gccacgattc ccgactacat caagcagaac ggcatcacag aatcgccggt gaagcgtggc
     7501 gatccagggt ctcccgaaat caaccttccc gtcccacccg gatgggagga catgggcgac
     7561 ggaacccctc cgtgggcgtg gagcggaatg aagttcaccg gtgacccggc gatggccgcc
     7621 agcccgccga ccatccttgc gctgctctcg aagctgcagg gcaacgtcga cccggccaag
     7681 atcctcgaat tcgcacccgg cgagatgaag aacctgccgg gctatgaggg cgacggcgtc
     7741 ggctcggcca gcacactcgg cggcttcgac gcctggcagg tcggcggcat gtacacgaag
     7801 gacggcgtca agcatgccgt cgcgcagaag acggtggtga tccctggcgc cgacgggctg
     7861 ttcgtcctgc aactcaacgc cgacggcacc gaggatcaga tgggcgctct catgggagcc
     7921 accaacgtga tcgacgagca aaccaccatc actccggcgc cgtgactgcg gtccagcctc
     7981 ccgctccgct cgcgcgtgtc ttctactccg gcttcaccag tcatcagatc gagcgcgtac
     8041 gccgtccgac cgtcgtcgtc aacgcgccgt agtcaggctc gtgagaccgc cgacgcgtcg
     8101 gccacctgct cggctgtcgg tcgcacaccc gtgtaacgct cgaactgctc ggcggcctgc
     8161 agcgcgatca cttcggcgcc cgtgatcacg ttgactcccg cggcacgggc agcagcgatc
     8221 agcggggtct ccgacggcag ggcgaccacg tcgaatacgg tgtccgcctt ggcgatcgcc
     8281 gacgtgtcga aggggctcag ccgctcctcc ggtccacccg ccatgccgac cggggtcaca
     8341 ttgacgatga catcggcggt tcgcgaaccc acctcggccg catactcgta cccgagctgg
     8401 cgcgccagcg cggcccccgc ctcggtgttg cgggcgacga tcaacccctt ctgaaagccg
     8461 ctgtcccgga acgccgcacc gaccgcgccg gccattccac cgctgccgtg aatcagcacc
     8521 gactgtgacc gctgcagtcc gtgctcctcg atgagccgct gcaccgcgat gtagtccgtg
     8581 ttcgatgcgg tgaggtgtct gccgtcgttg acgattgtgt tgacggcgtt gatcgcctga
     8641 gccgactgct ccacgcggtc gacaagcggg atgacgtcct gcttgaacgg catggacacc
     8701 gagcagcccc ggatgcccag cgcccgtacg ccaccgatcg ccccgacgat gtcagtggtg
     8761 gtgaacgcct tgtagacgaa gtcgagcccc agcaggtcgt agagatagtt gtggaacctg
     8821 gtgccgatat tgctcggcct gcccgcaagc gagatgcaca cccgggtgtc cttgctcagt
     8881 ggtgggcgca tcagccgacc gcccccagga cttgccttgc gacaccgtga atttcgcgat
     8941 agagctcctt ggacatgccc agatcgcgca gcgtcggcac atcgatgacc gtcgtcacca
     9001 cccccagatc ttcacgctgc cactgcgcgc cgaaccggtc aaggaccttg cgcatcgcaa
     9061 ggttgtcgga cagcacccgc gccgagaagc ggcgcacccc gtcgctacgc gcggcaaccg
     9121 ccaaggctgc catcagaaat gagccgacgc cacggccctg gtagtcgtcg ccgacgatga
     9181 aggccacctc cgcaagcgtc cgatcggttt cgtcgcgcac gaaccgggcg tccgccacca
     9241 cgggcccttc cccgccttcc gtcatcaccc acacgaagtg gtccttgtag tcgacctcga
     9301 acaggtaggt catcaaggat tggctcggcg tacgaggcgt ctggaatctg cggtacaacg
     9361 tttcgctgga gaactcgatg gtgccttccg agcttcgttc gctatcaccc ggcaggacgg
     9421 gccgcaggta aagccaagag tcgtcgcgca tttgaatcgg aatcggcgtg atgaaggcag
     9481 ccagtcgctg gcgcgccgta cgcaccagct tgtccatcat gcccggcacg tcgagcatgg
     9541 cggcgaaggc gccgtgcccg ccgacccagc ccgtcagcgg ctcggtcgcg accacagtgg
     9601 cggtccgtgg cgcctctcgc aacagcgcga tctcgccgac gatgagcggc ggcgacagct
     9661 ccacggcgat gggcacgccg tcgttgccgg tgtgcgtgat ctccgccttc ccggcgccga
     9721 tcaacaggaa cgattccgcg cgttcgccct gacgcatcaa gacccggccc gccggcgcat
     9781 tcagcggacg cagctgcgcc gccagtgaca ccagagcatc aggacgacag cccttgaaga
     9841 tctccagggc agcgagttcg tcggcgctga cggccgtcaa tccggccacg tcccgaggct
     9901 accgccgatg gctagcggtg tccgtccggc gcggagatct ccgtggcctc ggatcggccc
     9961 cgcaacacct cgctgccctt ggcggcccaa tgcgtgcgtt cggccgtatc ggcgcgctcg
    10021 atggcagccg ccgagcacag aatccgtcga tccgacgtct tcgcgaggtc ggccaggcgc
    10081 gccgcctcgt tcaccgggtc gccgatcacg gtgtactcgt agcggttctc cgcaccgatg
    10141 ttgcccgcga ataccggacc cgccgacaca ccgatcccaa agtccaccag cggcagctcg
    10201 cgcagttgct ccccgagggc gcgcgccgtc gccagcgccg aggacgccgc gccgttgctg
    10261 cgccagggcg ccccgaacac ggcgagtgcg gcgtcgccct gaaacttgtt gatcatcccg
    10321 tcacgggcgt cgacggcgtt gacgacgata tggaagaact cgttgaggac ctcggctacc
    10381 cgctgcggcg gatgactcgc ggccagcgcc gtcgagccgg tcaggtcgat gaagaggatc
    10441 gccgcctcct ggacgtcacc cgacatcgcg gcgccctctt cgatggcgcg aagggcaacg
    10501 ttggtgccga cgtgacggcc gaacaggtcg cgtacccgct cccgctcggc cagcccggcg
    10561 accatctgat tgaaaccgct ctgcagtcgg ccgatttcgg atcgttcgaa gacatccact
    10621 gacgtgtcga aacgccctcg ctcgacttcg gccatggcgt cgacgacttc gcctatcgga
    10681 tcggcgatcg atctcgacgt gaggatcatc gtcggcagcc cgaggacgac cgccgccgat
    10741 gccaccacca gggctgcgat ctcgaccgat gccgtcttcg ggatgagcca gccgttcgaa
    10801 cgcaccacga ccagcgccac gatgaccagg catggcaatg cactgcacag caaccacatc
    10861 agaatcaggc gtgcgaagac gccgggtgcc gtgacgaggc gctcagacgc gtgggtcgcg
    10921 gcggcaagga gcgggcgaat gaggttcatc gtcaatagaa ggccgatgcc cgtcgcggcg
    10981 gatccgccca gcacgacacc gaacgcggtt gcggcgacaa tgccggtccc gccaccgaca
    11041 ttgagcagca cggagattgc gccgcttacc acccacgtac c
//
LOCUS       DASYLX010000099         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3296383,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000099 DASYLX010000000
VERSION     DASYLX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296383"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VGP27_02445"
     CDS             <1..125
                     /locus_tag="VGP27_02445"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217526.1"
                     /note="catalyzes the formation of D-fructose
                     1,6-bisphosphate from D-fructose 6-phosphate in
                     glycolysis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV7850208.1"
                     /translation="YGMMVSLRGQEIGRVPLADAVRQLKLVPQSRYDDAAEFFG"
     gene            171..1676
                     /gene="gatB"
                     /locus_tag="VGP27_02450"
     CDS             171..1676
                     /gene="gatB"
                     /locus_tag="VGP27_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV7850209.1"
                     /translation="MTAASTAELVDYDDVVARYDPVLGLEVHVELSTATKMFCGCANR
                     FGAEPNTLVCPVCLGLPGSLPVLNQVAVESAMRIGLALNCEIVSWCRFARKNYFYPDM
                     PKNYQISQYDEPIAINGYLDVPLDDGTTWRVEIERAHMEEDTGKLTHLGSETGRIAGA
                     TTSLIDYNRAGVPLIEIVTRPIEGTGARAPEIARAYVTALRGMLRALGVSDVRMDQGS
                     MRCDSNVSLKPAGATEFGIRTETKNVNSLKSVEVAVRYEMRRQAAVLDAGDRVHQETR
                     HFHEDGYTSPGRSKETAQDYRYFPEPDLEPIAPDADFVEKLRETIPEYPWLQRKRIQS
                     DWGVSDEVMRDLVNAGAVDLVAATVAQGAPSEAARAWWGNFLVQKANEAGVELDELAI
                     TPAQVAAVVVLIDEGKLSNKLARQVVEGVLVGEGEPADVMKARGLELVRDDSLIQAAV
                     DDALAANPDVAEKIRGGKVQAAGAIVGAVMKATKGQADAARVRELVLAACT"
     gene            1677..2471
                     /locus_tag="VGP27_02455"
     CDS             1677..2471
                     /locus_tag="VGP27_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase family protein"
                     /protein_id="HEV7850210.1"
                     /translation="MAAADAELLAVVERSPAASDVHDRAGWVGLFAADARVEDPYGSR
                     PHVGVKEIGRFYDTFIGPRQIIFHRDVDLAAGPAVVRDLMLEIVMAAGVTLNVPMHLR
                     YDLRPFDGDWQIERLRAHWELPTMVVQMLREGAKSLPVSLRLGGELLRNQGLGGTAGF
                     ASGFRRPGPRQKRCVEAFLDAAVAGDEMTARRALSRGAAVSVGEKTPTAVGEFVDSVR
                     GGRWFKMIAAGDTVSVSVSTPSGRGVVFCEIGDATTGISRVRHFCG"
     gene            complement(2483..3604)
                     /locus_tag="VGP27_02460"
     CDS             complement(2483..3604)
                     /locus_tag="VGP27_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7850211.1"
                     /translation="MNLHGPVRAVVALSCAALLVLSGCARFDSAQSQPFTTEPELQPG
                     PSSTPPPPPPLPPKPFPKECPAPGVMQGCLESTSGLIMLPDSQSALVAERVTGAVKEV
                     SVRAEPKVKTVIPVDGSGDGGLMDIVLSPTYSQDRLMYAYVSTPTDNRVIRIADGDIP
                     KPIFTGIPKGATGNMGALVFTSPTTLVVLTGDAGDPALAADPASPAGKVLRIEQPTTV
                     NQAPQTTALSGMGAGGGMCVDPSDGSLYIDDRTPTADRLQRVTKDSKVSTVWTWPDKP
                     GVAGCAALDGTVLVNLINTKQTVAVRLAPDTGSVTGEPEVVRQDVHGHAWALQSSPDG
                     NVWGATVNKTAGDSEKLDDVVFPVFPSGGGFPRSNADNT"
     gene            3741..4544
                     /locus_tag="VGP27_02465"
     CDS             3741..4544
                     /locus_tag="VGP27_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7850212.1"
                     /translation="MTSNPQWQRPDDLGRPGSASLVDPEDDLPSSNYAGDFETTAIPN
                     YRTGSQPGFGLIGEPEALPYAQAGDSQPLNPYAQPADIGPGYHDGPIDDRRGTQDLGL
                     MLLRWAVGALLIAHGLQKAFGWFGGPGLGGFEDRLTEVGYQHADMLTYIATGAQILAG
                     VLLILGLFTPLAAAGALAYLVNGLLVEAMAAHDAARLRDFLTDGHEYKVILIAAVVAI
                     VLAGPGRYGLDAGRGWARRPFAGSFAALLIGVGGGIALWVLLNGGNPLS"
     gene            complement(4583..4987)
                     /locus_tag="VGP27_02470"
     CDS             complement(4583..4987)
                     /locus_tag="VGP27_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7850213.1"
                     /translation="MAGVTPQPPPAPVEPVVIRISPTAHLAVGFLVLGLLSLALAWTW
                     FAPLFVIPAMLSAYIVRLRTTADPDTVTARNLLGSQTLRWEDIEGLRFSKGSWARAQL
                     KDGRELRLPAVTFATLPQLTEVSAGRVPNPYG"
     gene            5240..>5451
                     /locus_tag="VGP27_02475"
     CDS             5240..>5451
                     /locus_tag="VGP27_02475"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383012.1"
                     /note="acetolactate synthase large subunit; catalyzes the
                     formation of 2-acetolactate from pyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV7850214.1"
                     /translation="MSAPTTRPPDRSANPPYGDLAEANAAQSAAKAAGHGPSSQPKKV
                     APQQLTGAQSVIRSLEELDVEVIFGIP"
BASE COUNT          899 a         1771 c         1884 g          897 t
ORIGIN      
        1 agtacgggat gatggtgtcg ctgcgcggcc aggagatcgg ccgcgtgcca cttgccgacg
       61 ccgtccgtca gctgaagctg gtgccccaga gccgatacga cgacgccgcc gaattcttcg
      121 gctgatttct tcggcgagca ggagaaaagt gacccactaa gctgggaacc atgaccgctg
      181 catccactgc cgaactggtc gattacgacg acgtcgtcgc acgctacgac ccggttctcg
      241 gcctcgaggt gcacgtcgag ctgtccacgg cgaccaagat gttctgcggc tgcgccaacc
      301 ggttcggcgc cgagcccaac acactggtgt gtccggtgtg cctcggcctg cccggctcgc
      361 tgccggtgct caaccaggtc gccgtcgagt cggcgatgcg gatcgggctg gcgctgaact
      421 gcgagatcgt gtcgtggtgc cggtttgcga ggaagaacta cttctacccg gacatgccga
      481 agaactacca gatctcccaa tacgacgagc cgatcgcgat caacggctac ctcgacgtgc
      541 ccctcgacga cggcaccaca tggcgcgtcg agatcgagcg cgcgcacatg gaggaggaca
      601 ccggcaagct gacccacctc ggcagcgaga ccggccgcat cgcgggcgcg accacgtcgc
      661 tgatcgacta caaccgcgcg ggtgtgccgc tgatcgagat cgtcaccagg ccgatcgagg
      721 gaaccggtgc gcgtgcaccc gagatcgccc gggcctatgt gaccgccctt cgaggcatgt
      781 tgcgcgcctt gggtgtttcc gatgtgcgga tggaccaggg ctcgatgcga tgtgattcga
      841 acgtctcgct caagccggcc ggggcaaccg aattcggtat ccgcaccgag acgaagaacg
      901 tcaactcttt gaagagcgtg gaggtcgcgg tccgttacga gatgcgcagg caggccgcgg
      961 ttctggacgc cggtgaccga gtccaccagg agaccaggca cttccacgag gacgggtaca
     1021 cgtcgccggg ccgcagcaag gagacggcgc aggactaccg ttacttcccc gagcccgacc
     1081 tcgagccgat cgcacccgac gccgacttcg tggagaagct gcgcgagacc attcccgagt
     1141 acccgtggtt gcagcgcaag cgcattcaat ccgactgggg agtctccgac gaggtcatgc
     1201 gcgatctggt caacgcgggc gccgtcgatc tggtggccgc caccgtcgca cagggcgccc
     1261 ccagcgaggc cgcgagggcg tggtggggga acttcctggt gcagaaggcc aatgaggcag
     1321 gcgtcgagct cgacgagctg gccatcacgc ctgcacaggt cgctgcggtg gtcgtgttga
     1381 tcgacgaggg caagctgtcc aacaagctgg cccggcaggt ggtggagggt gtgctggtcg
     1441 gcgagggcga acccgccgac gtgatgaagg cgcgcggact ggagctggtc cgcgacgact
     1501 cactgatcca ggcggcggtc gacgacgcgc tggccgccaa tcccgacgtg gcggagaaga
     1561 tccgcggcgg caaggtgcag gccgccggag cgatcgtcgg cgccgtgatg aaggcgacga
     1621 agggccaggc cgacgcggca cgggttcgcg agctggtgtt ggcggcctgc acctagatgg
     1681 ccgccgcaga cgccgaactg ctggcggtgg tggaacgttc gcctgcggcg agcgatgtgc
     1741 acgaccgcgc gggctgggtc gggttgttcg ccgccgatgc gcgcgtcgag gacccgtacg
     1801 gttcccgtcc gcacgtcggc gtcaaggaga tcggccgctt ctacgacacg ttcatcggac
     1861 cgcgacagat catctttcac cgcgatgtcg acctggcggc cggccctgcc gtggttcgcg
     1921 acctgatgct cgaaatcgtg atggcggcgg gcgtgacgct gaacgttccg atgcatctgc
     1981 gctacgacct gaggccgttc gacggcgact ggcagatcga gcgactgcgc gcgcactggg
     2041 agctgccgac gatggtggtg cagatgctgc gcgagggcgc gaaatcgctg ccggtctcgc
     2101 tgcgcctggg cggcgaactg ctgcgcaatc aagggctcgg cggcacggcg ggtttcgctt
     2161 cgggcttccg gcggccggga ccacgccaga agcgttgtgt cgaggcattt ctcgatgcgg
     2221 ctgtcgccgg tgacgagatg acggcgcggc gggcactgag ccgcggggcg gctgtcagcg
     2281 tcggtgaaaa gacaccgacc gcagtcggcg agttcgtcga cagcgtgcgg ggcggccgat
     2341 ggttcaagat gatcgcggcc ggtgacaccg tcagtgtttc ggtcagcaca ccgtcagggc
     2401 gtggcgtcgt tttctgcgaa atcggcgatg cgacaacagg tatcagccgc gtccgccact
     2461 tctgcgggta gcgccaaacc gtttaggtgt tgtcggcgtt actgcgcggg aagccgccgc
     2521 ccgacgggaa caccgggaac acgacgtcgt cgagcttctc ggaatcgcct gcggtcttgt
     2581 tcaccgtcgc gccccagacg ttgccgtccg gcgacgactg cagcgcccac gcgtggccgt
     2641 gcacgtcctg gcggaccacc tccggctcgc cggtcaccga accggtgtcc ggcgccagtc
     2701 gcacggccac cgtctgcttg gtgttgatca ggttgacgag caccgtgccg tccagtgccg
     2761 cacatccggc gacaccgggc ttgtcgggcc aggtccacac cgtggagacc ttcgaatcct
     2821 tggtgacgcg ctgcaaccgg tcggcggtcg gcgtgcggtc gtcgatgtac agcgagccgt
     2881 ccgacgggtc gacgcacatg ccgccacccg ctcccatccc gctcagcgcg gtggtctgcg
     2941 gcgcctggtt gaccgtggtc ggctgctcga tgcgcagcac cttgcccgcc ggcgacgccg
     3001 ggtcggccgc cagcgccggg tcacccgcat caccggtgag cacgaccaac gtggtggggc
     3061 tggtgaagac cagggcaccc atgtttccgg tcgcgccctt ggggataccg gtgaagatcg
     3121 gcttgggaat gtcgccgtcg gcgatgcgga tcacccgatt gtcggtcggc gtgctgacgt
     3181 atgcgtacat cagccggtcc tgcgagtacg tcggcgacag cacgatgtcc atcagcccgc
     3241 cgtcgccgct gccgtcgacc gggatgacgg ttttgacctt tggttctgcc cgaacggaca
     3301 cctctttgac ggcgccggtg acgcgttcgg cgaccaatgc ggactggctg tcgggcagca
     3361 tgatcagccc gctggtgctc tccaggcagc cctgcatcac ccctggtgcg gggcactcct
     3421 tgggaaacgg cttcggtggc agcggagggg gcggcggtgg agtcgagctg gggccgggtt
     3481 gaagctcggg ttcggtggtg aacggctgcg attgggcgct gtcgaatcgc gcgcaccccg
     3541 acagcacgag caacgcggcg cacgacagtg cgacgacggc acgcaccggc ccgtgcagat
     3601 tcatgcccgc caggttacgg attgttcctc ggtgcgcgcc acgcggcgtt caggtgcgtc
     3661 gcctcggcac ctgcgaacta gggcacaaaa gcgccgcgcc aatccgcgat tcgccgatcc
     3721 tgaccactta ccctgggcag gtgaccagta atccccagtg gcagcggccc gacgacctgg
     3781 gccggcccgg gtcggcaagc ctggtcgacc ccgaggacga tctgccctcg tccaactacg
     3841 ccggtgattt cgaaaccacc gccatcccca actaccggac cggctcccag cctgggttcg
     3901 ggctgatcgg ggagccggaa gcgttgccgt acgcacaggc cggcgacagc caacccttga
     3961 acccctacgc acaacccgcc gacatcggcc ccggatacca tgatggtccg atcgatgatc
     4021 ggcgtggcac ccaggacctc ggcctgatgc tgctgcgctg ggccgtcggc gccctgttga
     4081 tcgctcacgg cctgcagaag gcgttcggct ggttcggtgg tcccggcctg ggcgggttcg
     4141 aggaccggct gaccgaagtc ggctaccagc acgccgacat gctgacttat atcgccaccg
     4201 gcgcccagat actcgccggt gtgctgctga tcctgggtct gttcaccccg ctggcggcgg
     4261 cgggggcgct ggcctacctg gtcaacggcc tgctcgtgga ggccatggcc gcccacgatg
     4321 cggccaggtt aagggatttc ctgaccgacg gccacgaata caaggtcatc ctgatcgccg
     4381 cggtcgtcgc aatcgtcctc gccggcccgg gcagatacgg gctggacgcc ggtcgcggct
     4441 gggcccggcg accgttcgcc gggtcgttcg ccgcgctgtt gatcggcgtc ggcggcggca
     4501 tcgcgctgtg ggtgctgctc aacgggggca acccactgag ctgacgcggg aacgcggacg
     4561 cgaggagcaa aacagcctga gctcagccgt acgggttggg cacgcggccc gcgctgacct
     4621 cggtcaactg cggcagggtt gcgaacgtca cggcgggaag ccgcagttca cgcccgtctt
     4681 tgagctgtgc ccgggcccat gatcccttgc tgaaccgcag gccctcgatg tcctcccagc
     4741 gcagggtctg gctgccgagc agattgcgtg cggtcacggt gtcgggatcg gcggtggtgc
     4801 gcagccgaac gatgtacgcc gacagcatcg cgggaatgac gaacaggggt gcgaaccacg
     4861 tccaggccag cgccagtgac agcagtccca gcaccaggaa gccgaccgcg aggtgcgccg
     4921 tcggcgatat ccggatcacg accggttcga ctggtgcggg cggcggttgc ggagtcacac
     4981 ctgccatcgt cccactgcgc ggtgcgcgtc aaggaatttg acggttgacc agtgcggaga
     5041 ctacggtcat ttgttgtgca gaaccttggc ttgctcgtag taattgggtt gcgcgttgat
     5101 accgcgcttg ccctgactcg ggcatagtcg caagtatcaa cgcgcaaccc tcgttcagcg
     5161 gcatcgctga cgggggtttt ttgttgcccc agcagttatt tgagagtcca caacacaatc
     5221 aacgacaaat gaggacatcg tgagcgctcc aacgacacga cccccggatc ggtcggcgaa
     5281 cccgccgtac ggggatttgg ccgaggccaa cgctgcgcaa tccgcggcga aggcagccgg
     5341 tcacggcccg tcgagccagc ccaagaaggt tgccccgcaa caactcaccg gagcccagtc
     5401 ggtcatccgg tcgttggagg agctcgacgt cgaggtcatc ttcggcatcc c
//
LOCUS       DASYLX010000100         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1760082,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000100 DASYLX010000000
VERSION     DASYLX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760082"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VGP27_02480"
     CDS             <1..881
                     /locus_tag="VGP27_02480"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV7850215.1"
                     /translation="ALGKPLPPRPFGITWSDADRPAHRRGVLDCLEAIAAGEIYQACV
                     CTQFAGRLEGAPIDFFVDAVARTSPARAAYVAGDWGAVASLSPELFLRLAGDAVTSSP
                     IKGTLPRTADPAALRASAKDVAENIMIVDLVRNDLSRVAVTGTVTVPELLAVRPAPGV
                     WHLVSTVSARMPVDVPIAALLDAAFPPASVTGTPKTRARQLLTGWEQHRRGVYCGTVG
                     LASPVAGCELNVAIRTVEFAADSSAVLGVGGGITADSDPDREWDECLHKAAPIVGAMR
                     TGGSGRAGSRRAVPAR"
     gene            complement(823..1560)
                     /locus_tag="VGP27_02485"
     CDS             complement(823..1560)
                     /locus_tag="VGP27_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850216.1"
                     /translation="MTLPALVLVHGGQHAADCWNPTVDEVRRRAPGLGVIALDLPGRR
                     DKPGDLLTATIDDWVDSLLADIEDAGVDDFVIVGHSLAGLTVPGVVAKLGPSRVREMV
                     LAAAFVPPAGKCVVDTLPGPVGWYARRKADTGMSGTLPISLAEFVFCNGMTPPQRTFN
                     RARFYAESPSIIFDKVDRSGMPDEVPRTWILTLRDRALPVASQRRSIAALGGVHTVLP
                     IDTCHNLMVSEPELLAEILLDRCRRYA"
     gene            complement(1557..2297)
                     /locus_tag="VGP27_02490"
     CDS             complement(1557..2297)
                     /locus_tag="VGP27_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850217.1"
                     /translation="MALPALVLVHGGGLTADSWKYTVDEIHRLAPELGVLTVDLPGRA
                     SKPGDVTKLTIADFAESAVNDIEDAGLGEIVLVGHSYAGLTVPGVATRLGSVRVREIV
                     LASAFVPPEGKSVVDTLTGPLAPVARRNVRRGELSETPALGVRFIYFNGVPRARRKFM
                     ADKLYPESTRMLAENVSRRGMPDDIPRTWILTLRDRALSTESQRRSIDAIGGVDTVIP
                     MDTCHCPMLSQPEMLAEILLERCRRYAK"
     gene            complement(2310..>3092)
                     /gene="rsmI"
                     /locus_tag="VGP27_02495"
     CDS             complement(2310..>3092)
                     /gene="rsmI"
                     /locus_tag="VGP27_02495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV7850218.1"
                     /translation="ASARLINALGGADVVAAEDTRRVRTLAKALDVRIAGRVVSLFDQ
                     NESTRVPALVDDIRGGATVLVVSDAGMPVINDPGYRLVAACVEAGLKVSCLPGPSAVT
                     TALAVSGLPADRFCFEGFPPRKHSARSTWLTGLASEERTAVFFESPRRLGAFLQDAVE
                     VLGPERRAVVCRELTKTHEEIVRGSLAELSEWAADGVLGEITVVLAGAEPQADLECLV
                     AAVHALVDDGARVKDACAEVVAANPGAPSKRELYDAVLRSRD"
BASE COUNT          499 a         1051 c         1050 g          492 t
ORIGIN      
        1 aagcgctagg aaagccgctg ccgccgaggc cgttcggcat cacctggagc gacgccgacc
       61 gtccagccca ccgccgcggg gtgctcgact gcctggaggc gatcgccgcg ggtgagatct
      121 accaggcatg tgtatgcacg cagttcgcgg gccggctcga gggtgcgccg atcgacttct
      181 tcgtcgacgc cgtggcgcgc acctcgcctg cgcgggcggc gtatgttgcg ggcgactggg
      241 gtgcggtggc gtcgttgtcg ccggaattgt tcctgcgact cgcaggcgat gcggtcacgt
      301 cgagcccgat caaggggaca ttgccgcgga cggccgatcc cgcggcgctg cgggcgtcgg
      361 ccaaggacgt cgccgagaac atcatgatcg tcgacctggt ccgcaacgac ctgagccgcg
      421 tcgcggtgac cggcaccgtg accgtgcccg aactcctcgc ggtgcggccg gctcccggcg
      481 tctggcatct ggtgtcgacg gtgtccgcgc gcatgcccgt cgacgtgccg atcgctgcac
      541 tgctggacgc agcgttccca cctgcgtcgg tcaccggcac gcccaagacc cgtgcccgcc
      601 agttgctgac cgggtgggag cagcatcggc gcggggttta ttgcgggaca gtgggtttgg
      661 cttcaccggt cgccgggtgt gaattgaatg tggcgatccg caccgtggag ttcgcagccg
      721 acagctcggc ggtgctcggc gtcggcggtg gcatcacggc ggactccgat cccgaccgcg
      781 aatgggacga gtgcctacac aaggcagccc cgatcgtcgg cgctatgcgt accggcggca
      841 gcggtcgagc aggatctcgg cgagcagttc cggctcgctg accatcaggt tgtggcaggt
      901 gtcgatcggc agcacggtgt gcacgccacc gagtgcggcg atcgacctgc gctgtgacgc
      961 cacggggagc gcccggtcgc gcagcgtgag gatccaggtc cgcggcacct cgtcgggcat
     1021 tccggagcgg tcgaccttgt cgaagatgat cgacggcgat tcggcataga aacgggcgcg
     1081 gttgaacgtc cgctgcggag gcgtcatccc gttgcagaag acgaactccg ccagcgagat
     1141 aggcagggtc ccggacatgc cggtgtcagc cttacggcgg gcataccaac ccaccgggcc
     1201 cggcagtgtg tcgactacgc acttgcctgc cggtgggacg aacgccgcgg cgagcaccat
     1261 ctcccttact cgtgagggtc cgagtttcgc cacgacgccg ggcacggtga gtccggccag
     1321 ggaatggccg acgatgacga agtcgtcgac tccggcatcc tcgatgtcgg cgagtagcga
     1381 gtccacccag tcgtcgatgg tggcggtgag aagatcgccg ggcttgtcgc gccggccggg
     1441 aaggtcgagt gcgatcaccc cgaggcccgg tgcgcgacgc cggacctcgt cgacggtcgg
     1501 attccagcag tcggcggcgt gctggccgcc gtgcacgagc accaacgcgg gcagtgtcat
     1561 ttcgcgtacc gccgacagcg ttcgaggaga atctcggcca gcatttcggg ctggctgagc
     1621 attggacaat gacatgtgtc catcggaatg acggtatcca cgccgccgat cgcatcgata
     1681 ctccggcgct gagattccgt cgatagcgcc cgatctcgta gcgtcagaat ccaagtgcgc
     1741 gggatgtcgt cgggcattcc gcgccgggac acattttcgg cgagcatccg cgtcgattcc
     1801 gggtaaagct tgtccgccat gaatttgcgc ctggccctcg gtacaccgtt gaagtagatg
     1861 aacctgactc caagagcggg cgtttcgctc agttcacctc ttctgacgtt gcgccgagca
     1921 accggagcta acggcccggt cagcgtgtcg acgaccgact tcccctcagg cggcacgaac
     1981 gcgctcgcga ggacgatctc gcggaccctg accgatccca gcctggtggc cacgccggga
     2041 acagtcagcc cggcatacga atggccgacg aggacgatct cgccgagacc ggcgtcctcg
     2101 atgtcgttga cggccgattc cgcgaagtca gcgatggtca atttcgtcac gtcaccgggc
     2161 tttgatgcgc ggcccggcag atctacggtg agcacaccca attcgggtgc caagcggtgt
     2221 atttcgtcga cggtgtactt ccacgagtcg gccgtgaggc ccccgccgtg cacgagcacc
     2281 agtgcgggca gtgccatggc gcctccgggt cagtcgcgcg agcgcagaac cgcatcgtaa
     2341 agctcgcgct tggacggcgc cccgggattc gcggcgacca cctcagcgca ggcgtccttg
     2401 acccgcgcgc cgtcgtcgac gagcgcgtgc accgctgcca ccaggcactc cagatcggct
     2461 tgcggctcgg cgcctgccaa caccacggtg atctcgccga gcacgccgtc ggcggcccac
     2521 tcggagagtt cggccagcga gccgcgaacg atctcctcgt gggtcttggt cagctcgcgg
     2581 cagaccactg cgcgtcgctc cggcccgagc acttcgacgg cgtcctgcag gaacgccccg
     2641 agccgtcgcg gcgactcgaa gaacacggcg gtgcgctcct cggaagccaa acccgtcagc
     2701 cacgtgctgc gcgccgaatg cttgcgcggc ggaaagccct cgaagcagaa ccggtcggcg
     2761 ggcaaccccg acaccgccag cgccgtcgtc accgccgacg ggccgggcaa acaactcacc
     2821 ttcaacccgg cttccacaca ggccgccacc aaccgatagc cggggtcgtt gatcacgggc
     2881 atgcccgcgt cgctgaccac caggactgtc gcgccacccc gaatgtcgtc gaccagcgca
     2941 ggcacgcgcg tcgactcgtt ctggtcgaac aggctcacga ccctgcccgc gatccggacg
     3001 tcgagggcct tcgccagggt gcggaccctt cgggtgtcct cggcggccac cacgtcggcg
     3061 ccgcccagtg cgttgatcag ccgggcggag gc
//
LOCUS       DASYLX010000101         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2199033,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000101 DASYLX010000000
VERSION     DASYLX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199033"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1654)
                     /locus_tag="VGP27_02500"
     CDS             complement(<1..>1654)
                     /locus_tag="VGP27_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HEV7850219.1"
                     /translation="RFGADIGALKTALGDALRDTAREAELQSYEVPADFLIETEPFSA
                     ANGLLSGVGKILRPRLKEHYGERLEQLYAELAAARVDELRALRDNADGRPVVDSVLRA
                     AQAVLGSTDIDVDPDDHFTDLGGDSLSALTFANLLRDLFDVDVPVGMIVGPTGDLRRL
                     AAYIEIERESGSNRPTFAGVHGQRATAVRAADLTLDKFIDADTLAAAASIAYVTGDPK
                     TVLLTGANGWLGRFLALELLERAAQTGGTVITVLRGRDEASARARLDAAFDSGDPDLL
                     ERFRRLADDHLEIVAGDIGEPQLGLDGATWDRLAHNIDQIIHPAALVNHVLPYSEEFG
                     PNVVGTAEIIRLAITAQIKPVTYLSTVAVAMSVAASDFVEDGDIRDVSPVRPVDSSYA
                     NGYANSKWAGEVLLREAHDLCGLPVAVFRSDMILAHTRYAGQLNVPDAFTRLIQSLLV
                     TGVAPRSFYETDAEGRAQRAHYDGLPVDFVAESIIALAAKPSGGYRSFDVMNPYDDGV
                     SLDTFVDWLIEAGHPIARIEDHDDWLHRFETALTALPDAQRRH"
BASE COUNT          269 a          547 c          563 g          275 t
ORIGIN      
        1 gtgccgtcgc tgggcatcgg gcagcgcggt cagtgccgtc tcgaaccggt gcagccagtc
       61 gtcgtgatcc tcgatgcgtg cgatcgggtg accggcttcg atgagccagt cgacgaacgt
      121 gtccagcgat acgccgtcgt catacgggtt catcacgtcg aaagaccggt acccgccgga
      181 cggcttcgcc gccagcgcga tgatcgactc ggcgacgaaa tcgaccggca gcccgtcgta
      241 gtgcgcccgc tgcgcccgac cctcggcgtc cgtctcgtag aacgaccgcg gcgcgacgcc
      301 ggtgaccaga aggctttgga tgagcctggt gaaggcgtcg ggcacgttga gctgtccggc
      361 atatcgggtg tgagcgagga tcatgtcgga gcggaacacc gcgaccggca ggccgcacag
      421 gtcgtgcgcc tcgcgcagga gcacctctcc ggcccacttg ctgttcgcgt agccgttggc
      481 atacgagctg tcgaccggcc gcaccgggct gacgtcgcgg atgtcaccgt cctcgacgaa
      541 gtcgctagcc gcgaccgaca tcgcgaccgc gaccgtcgac aggtaggtga ccggcttgat
      601 ctgcgccgtg atcgccaacc ggatgatctc ggcggtgccg accacattgg ggccgaactc
      661 ttcgctgtag ggcagcacgt ggttcaccag tgccgccggg tgaatgatct gatcgatatt
      721 gtgggccaac cgatcccacg tcgcaccgtc cagacccagt tgcggttcgc cgatgtcgcc
      781 cgcaacgatt tcgagatggt cgtcggccaa tcgacggaac cgctcgagca ggtcgggatc
      841 cccgctgtcg aaggcggcgt cgagccgtgc gcgggcgctg gcctcgtcgc gtccacgcag
      901 gacggtgatc acggtgccgc cggtctgcgc ggcccgctcc agcagctcga gcgcaaggaa
      961 ccgtcccagc cagccgttgg ctccggtcag gagaacagtc ttcggatcgc cggtcacata
     1021 agcgatcgac gctgcggcag ccaaggtgtc cgcgtcgatg aacttgtcca gggtgagatc
     1081 cgcggcgcgg accgcagtgg cccgttgtcc gtgcacgccg gcgaaggtgg gccggttcga
     1141 cccggattcg cgctcgatct cgatgtaggc ggccagccgt cgcagatcgc cggtcgggcc
     1201 gacgatcatg ccaaccggca cgtcgacatc gaagaggtcg cgcagcaggt tggcgaatgt
     1261 caacgccgac agtgaatcac cgccgagatc ggtgaagtgg tcgtcggggt cgacgtcgat
     1321 gtcggtcgat cccaggaccg cctgcgcggc ccgcaggacg ctgtcgacga caggacggcc
     1381 atcggcgttg tcgcgcagcg cccgtagctc gtcgacacga gccgctgcca gttcggcata
     1441 cagctgctcc agccgctcgc cgtagtgctc tttgagcctg ggccgcaata tctttccgac
     1501 tcccgagagc agcccgttgg cggcgctgaa cggctccgtc tcgatgagga agtcggcggg
     1561 aacctcgtag gactgcagtt cggcctcgcg cgcggtgtcc cgcagcgcat cgccgagtgc
     1621 agtcttgagc gctccgatgt cggcaccgaa ccgc
//
LOCUS       DASYLX010000102         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3844502,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000102 DASYLX010000000
VERSION     DASYLX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844502"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="kasA"
                     /locus_tag="VGP27_02505"
     CDS             complement(<1..681)
                     /gene="kasA"
                     /locus_tag="VGP27_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasA"
                     /protein_id="HEV7850220.1"
                     /translation="MTRPSTANGGYPSVVVTAVTATTSIAPEIESTWKGLLAGESGIR
                     VLEDEFVTKWDLPVRIGGHLKENVDDHMGRLDLRRMSYVQRMAKLLGGQIWDAAGTPE
                     VDGDRFAVVVGTGLGGGEKIVETYDLMNEGGPRKVSPLAVQMIMPNGAAAVIGLQLGA
                     RAGVMTPVSACSSGSEAIAHAWRQIVMGDADIAVCGGVEGGIEALPIAAFSMMRAMST
                     RNDDPEGAS"
     gene            complement(678..980)
                     /gene="acpM"
                     /locus_tag="VGP27_02510"
     CDS             complement(678..980)
                     /gene="acpM"
                     /locus_tag="VGP27_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="meromycolate extension acyl carrier protein
                     AcpM"
                     /protein_id="HEV7850221.1"
                     /translation="MAATQEEIISGLAEIIEEVTGIEPSEVTPEKSFVDDLDIDSLSM
                     VEIAVQTEDKYGVKIPDEDLAGLRTVGDVVSYIQKLEEENPEAAAALREKIEADRP"
     gene            complement(1066..1974)
                     /locus_tag="VGP27_02515"
     CDS             complement(1066..1974)
                     /locus_tag="VGP27_02515"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV7850222.1"
                     /translation="MLAMLAPGQGSQTPGMLASWLELPGAADRISTWSQSSGLDLARL
                     GTTATAEEITDTAVTQPLVVAATLLAWEELTKRGLLAGRDFIAAGHSVGEIAAYAVAG
                     VISSDDAVALAATRGAEMAKACAVEPTGMSAVLGGDEAEVLSRLEELDLVPANRNAAG
                     QIVAAGSLAALSKLSEDPPAKARVRALATAGAFHTHYMASATDGYAAAAERVATSEPT
                     ATLLSNADGQPVASGSDAMSKLVAQLTRPVRWDLCTETLRNSNVTAIVEFPPAGTLAG
                     IAKRELKGVSTHAVKGPADLDGLTEL"
     gene            complement(2103..3389)
                     /locus_tag="VGP27_02520"
     CDS             complement(2103..3389)
                     /locus_tag="VGP27_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV7850223.1"
                     /translation="MADNRFIPPASTLEVLETVPESVLRRLNQYSGRLATQAVHVLEE
                     RLPFFADLEASQRASVQLVVQTAVVNFVEWMHNPNSDVSYTAQAFEVVPQDLRRRIAL
                     RQSVEMVRVTMEFFEEVVPLLARSEDQLTALTAGILRYSRDLAFAAATAYADQAEARG
                     AWDTRMEANIVDAVVRGHTGPDLQSQAAALNWDATAAATVIVGMPQPDRLDLASDDVH
                     DVVNRHGRAALSDVHGTWLVTIVSGPLTLTDRFLGELIGVFADAPVVIGPTAPTLSAA
                     HWSATEAIAGMNAVAGWTGAPRPVAARELLPERALLGDASAIAALETEVMRPLGDAGP
                     ALTETLDAYLDSGGAIEACARALFVHPNTVRYRLKRIADFTGRDPTTPRDAYVLRVAS
                     TVGRLTRQAQHSSTSNVPAGQITKPPEATVEADRAG"
     gene            complement(3442..>4537)
                     /locus_tag="VGP27_02525"
     CDS             complement(3442..>4537)
                     /locus_tag="VGP27_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEV7850224.1"
                     /translation="FRQLGIFAAEGQKYEPVDKDQVMFYREDKQGQILEEGINEAGAF
                     SSWIAAATSYSHSNEVSVPFYIFYSMFGFQRIADLAWAAADQRSRGFLLGATAGRTTL
                     TGEGLQHADGHSLLLASTNPAVVAYDPAFAYEIAYIVESGLARMFGEDPENVYFYITI
                     YNEPYKQPAEPEGLDVEGLLRGIYRYRPAPEQKSKQAHILVSGVAMPEAVRAAEMLAA
                     EWDVAADVWSVTSWGELNRDGVEIEKEKLRHPDRPARVPYITQALADTRGPAVAVSDW
                     MRAVPEQIRPWMPNSYATLGTDGFGFSDTRPAARRYYNTDAESVAVAVLEALAADGDI
                     DQSVAIEAAKKYEIDDVLAAPEQTSDPGVA"
BASE COUNT          710 a         1528 c         1522 g          777 t
ORIGIN      
        1 agaagcgccc tcggggtcgt cgttgcgggt cgacatggcg cgcatcatcg agaacgccgc
       61 gatcggcagc gcctcgatgc cgccctcgac accgccgcag acggcgatgt cggcatcgcc
      121 catgacgatc tgccgccacg cgtgggcgat agcctcggag cccgacgagc aggccgacac
      181 cggtgtcatg acgccggcgc gggcaccgag ctgcaggccg atgaccgccg cagcaccatt
      241 cggcatgatc atctgaacgg caagcggcga caccttgcgg gggccgccct cgttcatcag
      301 gtcataggtc tcgacgatct tctcgccgcc gcccaggccg gtgccgacaa caacggcgaa
      361 gcgatccccg tcgacctcgg gtgttcccgc ggcgtcccag atctggccgc cgagcagctt
      421 ggccatccgc tggacatacg acatccgccg caggtccagc cttcccatgt ggtcgtcgac
      481 gttctccttg aggtggccgc cgatacgcac gggcaggtcc cacttggtga cgaattcgtc
      541 ctcgaggacg cggattccgc tctcgcctgc caacaagccc ttccacgtgc tctcgatttc
      601 cggcgcgatc gatgtcgtcg ccgtgacggc ggttaccaca acgctggggt aaccgccgtt
      661 agcagtggaa ggcctggtca cgggcgatcc gcttcgatct tctcgcgcag cgcggcagcg
      721 gcctcagggt tttcttcctc gagcttctgg atgtagctga cgacgtcacc gacggtgcgc
      781 aggcctgcga ggtcctcgtc ggggatcttc acgccgtact tgtcctcggt ctgcacggcg
      841 atctcgacca tcgacagcga gtcgatgtcc aggtcgtcga cgaacgactt ctccggggtg
      901 acctccgacg gctcgatgcc ggtgacctct tcgatgatct cggcgagacc ggagataatt
      961 tcttcttgag tggcggccac tatggctcct tctaattggt tgtgttactg cgatttacgt
     1021 tgttatgcgg ttgtactgac tccagcggcg ccggaagtca cacctctaga gctcggttag
     1081 cccgtccagg tcggcagggc ctttgacggc atgcgtcgaa acccctttga gttctcgctt
     1141 ggcgattccg gcgagtgtcc cggcgggcgg gaactcgacg atcgcggtca cgttcgaatt
     1201 gcggagcgtc tccgtgcaca gatcccatcg caccggccgg gtcagctggg cgaccagctt
     1261 gctcatggca tcggaccccg aggcgacggg ctgtccgtcg gcgttcgaca gcagggtggc
     1321 cgtcggctcg gacgtcgcaa cgcgctccgc ggcggcggcg tagccgtcgg tggccgacgc
     1381 catgtaatgc gtgtggaacg cgcccgcggt ggcgagcgcg cggacccgcg ccttggcggg
     1441 cggatcctcg gacagcttgc tcagtgcggc aaggctgccc gccgccacga tctgaccggc
     1501 cgcattgcgg ttcgcgggca ccaggtcgag ttcctcgagg cggctcagca cctcggcctc
     1561 gtcgccgccg agcaccgcgg acatgccggt gggctccaca gcacaggcct tggccatctc
     1621 ggcaccgcgg gtggcggcca gcgcgacggc gtcgtcgctg gagatcactc ctgcgaccgc
     1681 gtacgcggcg atctcgccaa cggagtggcc ggctgcgatg aagtcccggc cggccagcag
     1741 gccgcgcttg gtcagctctt cccaagccag cagcgtcgcc gccaccacca gtggctgcgt
     1801 caccgcggta tcggtgatct cctcggccgt cgcggtggta ccgaggcggg ccaggtcgag
     1861 accgctggac tgcgaccagg tggagatgcg gtcagccgcg ccgggcagct caagccagga
     1921 ggcgagcatg ccgggggtct gagatccctg gccgggagca agcattgcga gcacgtctct
     1981 aaggaaacac tgagggaacg gatctgcgcc gtgtaagaga ttatgaacct ggtcttatct
     2041 ttttgtggag acccgacaaa acagcatgtg atgggactcc atcgatatca ggccgaaatc
     2101 tgttaccctg cccggtccgc ctcaaccgtg gcctccgggg gcttcgttat ctggccagcg
     2161 ggcacattcg acgtgctgga atgctgtgct tggcgcgtca ggcggcccac tgtcgaggcc
     2221 acccgaagga cataagcgtc gcgcggagtg gtcggatcgc ggccggtgaa gtccgcgatg
     2281 cgcttgaggc ggtaccgcac ggtgtttgga tgaacgaaca gtgcgcgcgc acatgcctcg
     2341 atcgcgccgc cggagtccag atatgcgtcc agggtctcgg tcaacgccgg tccggcgtcg
     2401 ccgagcggcc tcatcacctc ggtctccaac gccgcgatcg ccgacgcgtc gcccagcagc
     2461 gcccgctcgg gcaacagttc gcgggcggcg acgggccgcg gcgcccccgt ccagccagcc
     2521 accgcgttca tcccggcgat cgcttcggtg gcgctccagt gcgcggccga cagcgtgggt
     2581 gcggtcggcc cgatgacaac cggcgcgtcg gcgaagacgc cgatcagttc gccgaggaaa
     2641 cggtccgtca gcgtcagcgg gcccgacacg atggtcacca gccaggtgcc gtgcacgtcg
     2701 gaaagggctg cacgtccgtg cctgttgacc acgtcgtgga cgtcgtcgct ggccaggtcg
     2761 aggcggtcgg gctgcggcat gccgacgatg acggtggcgg ccgcggtggc gtcccaattg
     2821 agcgcggcgg cctgggactg caggtccggt ccggtgtggc cgcggaccac cgcgtcgacg
     2881 atgttggcct ccattcgggt gtcccaggcg cctcgcgcct ctgcctggtc ggcgtaggcg
     2941 gtggcggcgg cgaacgcgag atcgcggcta taccggagga ttccggccgt caatgccgtc
     3001 agctggtcct ccgagcgggc cagtagcggc accacttctt cgaagaactc catggtgacc
     3061 cgcaccatct ccacggactg ccgcaacgcg atgcgtctgc gcagatcctg cggcacgacc
     3121 tcgaatgcct gcgccgtgta gctgacgtcg ctattggggt tgtgcatcca ctccacgaag
     3181 ttgaccaccg cggtctgcac caccagctgg acgctggcgc gctgcgacgc ctcgaggtcg
     3241 gcgaagaacg gcagccgttc ctcgagaacg tgcactgcct gcgtggccag ccgcccggag
     3301 tactggttca accggcgcag caccgattcg gggacggtct ccagcacctc cagcgtggaa
     3361 gcgggcggga tgaaccggtt gtcggccatc cctaaaagct acgccacctt tatggcggaa
     3421 tcctccaagg acggtcgcgg gtcacgccac cccgggatcg gaggtctgct ccggcgccgc
     3481 cagcacgtcg tcgatctcgt actttttggc ggcctcgatg gcgaccgact ggtcgatatc
     3541 gccgtcggcg gccagcgcct ccagcaccgc caccgccacc gactcggcgt cggtgttgta
     3601 gtaacgccgc gccgcgggcc gggtgtcgga gaagccgaag ccgtcggtgc ccagcgtggc
     3661 gtaggagttg ggcatccacg gccggatctg ttctggcaca gcacgcatcc agtccgatac
     3721 ggccacggcg ggaccgcggg tgtcggcgag cgcctgggtg atgtagggca cccgcgccgg
     3781 acgatccgga tgccgcagct tctccttctc gatctccacg ccgtctcggt tcagctcgcc
     3841 ccagctggtg accgaccaga cgtcggcggc cacgtcccat tccgcggcca gcatctcggc
     3901 ggcccgaacg gcctccggca tggccactcc cgacaccagg atgtgggcct gcttcgactt
     3961 ctgctccgga gcgggccggt agcggtagat gccacgcaac aagccctcca cgtccagccc
     4021 ctccggttcg gccggctgct tgtacggctc gttgtaaatg gtgatgtaga agtagacgtt
     4081 ctccggatcc tcgccgaaca tgcgggccag gccactctcg acgatgtagg cgatctcgta
     4141 ggcgaacgcc gggtcgtagg ccaccaccgc cggattggtc gaagccagca gcagcgagtg
     4201 gccgtcggcg tgctgcaggc cctcgccggt gagcgtggtg cggcccgcgg tggcgccgag
     4261 caggaagccg cgcgaacgct ggtcggccgc ggcccaggcg agatcggcaa tgcgctgaaa
     4321 gccgaacatc gagtagaaaa tatagaacgg cacgctcact tcgttggaat ggctgtacga
     4381 agtggccgcc gcgatccacg acgagaaggc gcccgcctcg ttgatgcctt cctcgaggat
     4441 ctggccctgc ttgtcctcgc ggtaaaacat cacctggtcc ttgtccaccg gctcgtactt
     4501 ctggccctcg gcggcgaaga tgccgagctg gcggaac
//
LOCUS       DASYLX010000103         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3844508,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000103 DASYLX010000000
VERSION     DASYLX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844508"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGP27_02530"
     CDS             <1..651
                     /locus_tag="VGP27_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEV7850225.1"
                     /translation="SLVHALVADPEMREGQMVAELERLWEKLPFESAWYADNELARHR
                     LMLSTFAQWREQTRGQLTEVGTEVDVDGVLDCDGAAVRVRGRLDRLERDEAGRLVVVD
                     IKTGKSPVTKDDAQCHAQLAMYQLAIAEGLLPEGDTPGGGRLVYLGKTGSAGPTERQQ
                     DPLTADSRGQWRELVGTAAAATQGPQFLARINEGCAHCPVRQNCPAQASTDGAPNP"
     gene            648..>1514
                     /locus_tag="VGP27_02535"
     CDS             648..>1514
                     /locus_tag="VGP27_02535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEV7850226.1"
                     /translation="MTPKYSPDELASALGLFAPTEEQAAVIAAPPGPLVVIAGAGAGK
                     TETMAARVVWLVANGYASPGEVLGMTFTRKAAGQLLRRVRSRLARLAGTGIVAGGMDA
                     ADDPATVSTYHAFAGTLLREHGLLLPVEPDTRLLSETELWQLAFRVVTEFPGALDTDK
                     TPAAVTMMVLRLAGALAEHLVDTEQLTDTHVELERLVHTLPAGPYQRDRGPSQWLLRM
                     LATQTERTELVPLIDALHRRMRAEKVMDFGMQMASAARLASTFPQVGEQLRRRFRVVL
                     LDEYQDTGHAQRI"
BASE COUNT          240 a          502 c          548 g          224 t
ORIGIN      
        1 tcgttggtgc acgccctggt cgcggatccg gaaatgcgag agggtcagat ggtcgccgag
       61 ctcgagaggc tctgggagaa gctgccgttc gagtcggcgt ggtacgccga caacgagctg
      121 gcccggcacc gcttgatgct gtcgaccttc gcgcagtggc gggagcagac acgcgggcag
      181 ctcaccgagg tgggcaccga agtcgacgtc gacggcgtgc tcgactgcga cggggcggcc
      241 gtacgcgtgc gcggcaggct cgaccggctc gagcgcgacg aagcgggccg cctggtggtc
      301 gtcgacatca agaccggtaa gtcgcccgtg accaaggacg atgcgcaatg ccacgcgcag
      361 ctggcgatgt accagctcgc catcgcagaa ggccttctgc cggaaggcga tacacccggc
      421 ggcggcaggc tggtctacct gggcaagacc ggctccgctg ggccgaccga gcgtcagcag
      481 gacccgctga cggcggattc gcgcggccag tggcgtgagc tggtgggtac ggctgcggcg
      541 gcgacgcagg ggccgcagtt cctggcaagg atcaacgagg gctgcgcgca ctgcccggtg
      601 cggcagaact gccccgccca agcctcgacc gacggagcgc ccaacccgtg acaccgaagt
      661 acagtcccga tgagttggcc tctgctcttg ggcttttcgc gccgaccgaa gagcaggccg
      721 cggtcatcgc cgcaccgccc ggtccgctgg tggtgatcgc aggagcgggt gccggcaaga
      781 cggagacgat ggctgcccgg gtggtatggc tggtggccaa cggctacgcc tcacccggtg
      841 aggtgctcgg catgacgttc acccgcaagg cggccggcca attgttgcgc cgcgtccgtt
      901 cccggctggc ccggctggcg ggcaccggca tcgttgccgg cggcatggac gcggccgacg
      961 accccgcgac ggtcagtacc tatcacgcgt tcgcgggcac gctgctgcgc gagcacggcc
     1021 tgctgcttcc ggtcgagccc gacacccggc tgctcagcga aacagagttg tggcagttgg
     1081 cgtttcgcgt cgtcaccgag ttcccgggcg cacttgacac ggacaagacg ccggccgccg
     1141 tcaccatgat ggtgctgcgg ttggccggag cgttggccga acatctcgtc gacaccgaac
     1201 aattgaccga tacccacgtc gagctggagc ggctcgtgca cactttgccc gccgggccgt
     1261 atcagcgcga ccgcggaccc agccaatggc tgctgcggat gttggccacc cagaccgagc
     1321 gtaccgagct ggtgccgctg atcgacgcgt tgcaccggcg gatgcgggcc gagaaggtga
     1381 tggacttcgg catgcagatg gcgtcggccg cccggctcgc gtcgacgttc ccgcaagtcg
     1441 gcgagcagct gcggcgccgg ttccgtgtcg tgctgctcga cgagtaccag gacaccggcc
     1501 acgcgcagcg gatc
//
LOCUS       DASYLX010000104         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_115015,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000104 DASYLX010000000
VERSION     DASYLX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115015"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VGP27_02540"
     CDS             complement(<1..827)
                     /locus_tag="VGP27_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7850227.1"
                     /translation="MVKCLENEGVTHVFGIPGEENIRFVDALSRSSIEYVLTRHEQGA
                     SFMAEVYGRLTGRAGVCSATLGPGAINLLLGVADATTNSTPLIALSAQVGMGRSYKES
                     HQGVDLVSMFAPATKWSALVATPSAVPEMVRKAFKLAQTERPGAVYLAVPEDVEEAAA
                     PAGSGPLPVNVPRPDDPSAAQLERAAEILRHARSPILLAGHGAARSGATEAVRRFAEA
                     LDIPVATTFHGKGVMPDDHRLSLGAVGFMRHDYVNFGFDRADAIIAVGYELQEFDPVR
                     "
     gene            complement(940..1737)
                     /locus_tag="VGP27_02545"
     CDS             complement(940..1737)
                     /locus_tag="VGP27_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEV7850228.1"
                     /translation="MDDDVPGGRTRVVTSRREVLKYSLGAPLLAGLAALTFDEPRASA
                     ANAKLIDFAERLVPADQIAAAGYAGALVYVSELRPGATFDFKPVTREYADALRASGLH
                     VVSCYQYGKPGWPTPSDFTRGYDGGVADAQTAMRLHAAAGGPDSAPIFFSIDEDIDAD
                     TWKTVALQWLRGINSALGVQRTGVYGHAGVCGWAIADGVIGHSSTPGYRWAWQTRAWS
                     HGEREPAAVLYQNVIVTAADPGVSLGGTHVDEDDILAADFGQWDLAR"
     gene            complement(1756..>3310)
                     /locus_tag="VGP27_02550"
     CDS             complement(1756..>3310)
                     /locus_tag="VGP27_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV7850229.1"
                     /translation="VASASTLTQLYIGVNTPVVIEPGIVALGIGLGVVLAILAALLPS
                     LSAQRTAIASELSGRAAQETTKSRSIWPKAVGLLALGAAGVFAARFAAESGGLEPWQA
                     AVSGAGVVVAVVGMLTAAAYLSAQLISMVRLGPARARGATFRIALTGLRANPTRTTAI
                     AGAVAVPVAVAMLLSSFLVGINSGSANLARTQASDRLVVTTSRFNDWGALDAKVSPDT
                     IAKLGALPGVERVEQMVEIEMALADGSAAYMRAESDPTFPFPVLAGQSPKATLDANQL
                     VIGGILAREENLRVGDTILLGSRPQAQKMVIGTIVATPEVGGRRIYMPFDAAKQIFGE
                     QPPGLVRIKAAPGVPLDQVAAEINSTQFNQLVKVTDSAGYEQATASGLGRFLTTLNAL
                     KYGLLAIAFVSVSSTLLLVGMRRRREMALVQALGATRSKVFSVTTIEALIASGVGAVL
                     GAVLSVAIMEAVRQAAVVNVGSVSPLTFPWLEALKYAALAAAAAVFAAIVPAWKSTKA
                     APSTALRDE"
BASE COUNT          573 a         1094 c         1100 g          543 t
ORIGIN      
        1 cggaccggat cgaactcctg caattcgtaa ccgacggcga tgatggcatc ggcccggtcg
       61 aagccgaagt tcacgtagtc atggcgcatg aaacccaccg cgccgagcga cagccggtgg
      121 tcgtcgggca tcacgccctt accgtggaac gtggtggcca ccgggatgtc gagcgcttct
      181 gcgaaccggc gtaccgcttc ggtggcgccg ctgcgagctg cgccatgacc ggccaacagg
      241 atggggctgc gtgcgtgccg caggatctcc gcggcccgtt ccagttgagc ggccgaggga
      301 tcgtcgggcc gcggaacgtt gaccggtaat gggcccgacc cggcaggggc agcggcttcc
      361 tcgacgtcct cgggaacggc gagatagacc gctcccgggc gctcggtctg tgcgagcttg
      421 aaggccttgc ggaccatctc cggtacagcg gacggtgttg caacaagtgc ggaccacttg
      481 gtcgcgggcg cgaacatcga aaccaggtcc acgccttgat gcgactcctt gtagcttcgg
      541 cccatgccga cctgcgccga aagagcaatg agtggcgtcg aattcgtggt ggcatcggcc
      601 acgccgagca gaaggttgat cgcaccaggg ccgagcgttg ccgagcacac gccggctcgg
      661 ccggtcaagc gtccatagac ctccgccatg aacgaggcac cctgctcgtg gcgcgtcagc
      721 acgtactcga tcgacgatcg agacagcgcg tcgacgaatc ggatgttctc ctcgccgggt
      781 atcccgaaga cgtgcgttac gccttcgttc tcaagacact tgacgatgag ctcggcggtc
      841 gagcgactcg attcgtccac acctcgagcc tatgtcagcg ctgcggtgcg tagacccgta
      901 tgaccgagcc gtttcggggc cctggtgacg ccgtcctgat cagcgagcga gatcccattg
      961 gccgaaatcg gctgccagga tgtcgtcctc gtcgacgtgg gtaccgccca ggctgacgcc
     1021 cgggtcggcc gcggtgacga tgacgttctg gtaaagcacc gcagccggtt cgcgctcgcc
     1081 atgtgaccag gctctggtct gccatgccca ccggtatccc ggagtggagg agtgcccgat
     1141 gacgccgtcc gcgatcgccc acccgcacac tccggcgtga ccgtagacac cggtgcgctg
     1201 cacacccagg gcggagttga tcccacgaag ccactgcagt gcaacggttt tccaggtgtc
     1261 ggcgtcgatg tcctcgtcga tgctgaagaa gatcggggcg gaatccggac cgccggccgc
     1321 ggcatgcagg cgcatcgctg tttgagcgtc ggcgacgccg ccgtcgtagc cacgggtgaa
     1381 atccgagggt gtcggccagc cgggcttacc gtactgatag cagctgacga cgtgcagccc
     1441 cgacgcgcgc agcgcatccg cgtactcgcg ggtcaccggc ttgaaatcga aggtggcgcc
     1501 gggccgaagc tcggagacgt agacgagcgc gcccgcatac cccgccgccg cgatttgatc
     1561 cgcgggcacc aaacgctcgg cgaagtcgat cagcttggcg ttggcggctg acgcacgcgg
     1621 ttcgtcgaag gtcagcgcgg caagaccggc gagaagggga gcacccagcg agtacttgag
     1681 aacctcgcgt ctcgaagtga ctactcgggt cctgccaccg ggcacatcgt cgtccatgaa
     1741 gccattgtcg ctgacctact cgtcccgcaa cgccgtcgac ggtgccgcct tcgtgctctt
     1801 ccatgcaggg acgatcgctg cgaataccgc cgccgcggct gcgagcgccg catacttgag
     1861 ggcctccagc caggggaagg tcagtgggct gaccgatccg acattgacga cggccgcctg
     1921 gcgaaccgcc tccatgatcg ccaccgacag caccgcgccg agtacggcgc ccacgccgct
     1981 ggcgatgagg gcctcgatcg tcgtgaccga gaaaaccttc gatctggtgg cgccgagcgc
     2041 ctgcaccagc gccatctcgc gtcggcggcg catgccgacg aggagcagtg tcgacgacac
     2101 cgatacgaac gcgatcgcaa gaaggccgta cttgagcgcg ttcaacgtcg tgaggaagcg
     2161 gccgagaccg ctggcggtgg cctgctcgta gccggccgag tcggtgacct tgaccagctg
     2221 gttgaactgc gtggagttga tctcggcggc gacttgatcc agcggcacac ctggagccgc
     2281 tttgatgcgg accaggccag ggggctgctc cccgaagatc tgcttggcgg cgtcgaaggg
     2341 catgtatatc cgcctgccgc cgacttccgg cgtcgccaca atggtgccga tgaccatctt
     2401 ctgtgcctgt ggtctgctgc cgagcagaat cgtgtcgcct acgcgaaggt tctcctcgcg
     2461 ggcgaggatg ccaccgatga cgagctggtt ggcgtccagc gtcgccttcg gcgactggcc
     2521 ggccagcaca gggaacggga aggtggggtc actctcggca cgcatgtagg cggccgatcc
     2581 gtcggcaagc gccatttcga tctccaccat ctgctcaacg cgttcgacgc ctggaagggc
     2641 tccgagtttc gcgatggtgt cgggggacac cttcgcgtcc agcgcgcccc agtcgttgaa
     2701 tcgcgaggtg gtcaccacca ggcggtcgct tgcctgcgtt ctcgccagat tcgccgagcc
     2761 actgttgatt ccgacgagga agctcgagag cagcatcgcc accgccaccg gtacggcgac
     2821 ggcaccagcg atcgcggtgg ttcgggtcgg attcgcgcgc agtcccgtga gcgctatccg
     2881 gaatgtcgcg ccgcgcgccc ttgccggccc caatcgcacc atcgagatga gttgggcgct
     2941 gagatacgct gcggccgtga gcattcccac taccgcaaca acgacgcctg cgccggaaac
     3001 cgcagcctgc cacggctcca gcccgccgga ctcggcggcg aatctcgcgg cgaaaactcc
     3061 ggcagctccg agtgcgagca gcccgaccgc cttgggccag atgctgcgcg acttggtggt
     3121 ctcctgcgcg gcacgtccgg atagttcgga cgcaatcgcg gtccgctggg ccgacaggct
     3181 gggaagcagg gcggccagga tcgccagcac aacacccaag ccgatcccca acgccacgat
     3241 gccgggttcg atcacgacag gggtgttcac gccgatgtac agctgtgtca gtgtgctggc
     3301 actcgcgaca
//
LOCUS       DASYLX010000105         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_224355,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000105 DASYLX010000000
VERSION     DASYLX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224355"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3106
                     /locus_tag="VGP27_02555"
     CDS             <1..3106
                     /locus_tag="VGP27_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7850230.1"
                     /translation="GGVRPVEQGEGDSFVVAFARASDAAACALELQRAPLAPIRLRIG
                     VHTGQIQLRDEGNYAGPTINRTARLRDLGHGGQTLLSGATEPLVVDWLPADAWLTELG
                     THPLRDLPRPERVVQLCHPDTCNDFPPLRTSTTVDARNLPVQLTSFIGRGVQMDDVRR
                     LLGDNRLVNLTGAGGAGKTRLAVEIAARFGGDFPDGVHYVDLAPVTHAEVVPVAVARA
                     LGLPDQPGRSTVDSVIRSVGERRMLMVMDNCEHLLERAALLSNELLGACPSLRLLATS
                     REPLGVPGEVTFLVPSLSIGDEAIDLFTDRARRIRPDFAVTESNSSKVEEICRRLDGM
                     PLAIELAAARVRALSLDDILGSLHDRFRLLTGGARTAVRRQQTLRASVDWSHALLTES
                     ERVLFRRLAVFLGGFDLDAAQAVGGTTDVERYQVLDQLTLLVEKSLAVAENTSGRTRY
                     RLLETVRQYAQEKLGESGEAVDVRGRHRDHYLAIADVLNLPARPDYRDYVDRIGSDID
                     NLRAAFAWSMEAGDTEAALRLASSLFPIWQDGGRNGEGMAWLKAALTQADTAAASVDP
                     AIQARALTDKVMLAAWTLRVASLDEADRALALARDTSDSALAIRALIARGICTYYDEE
                     LAGPCFTEAAELAQGLNDPWIWSQLYMEQARSSIGAGYPAVVEVAATNALKVSAAIGN
                     HATTRFCRFAQGWAQAWRGDFHGALIKADEAIGEAAEAHDTMLQLYALLVQGFVRAQL
                     GDVEGARMSAEYALEVATDLKAFYVAPGQASIAVVHQAAGDAEAAQRAYAVAREDAGL
                     NRMAAGAFCFSALAPLACGDRHTAREWAEVDVEVSYGCYQSAALMTRAQVKLVDGEPE
                     GAQDDADAALSIATEVGIAHRMTMAFEVLATLATRAESHREAARLFGAAEAMRQRTGE
                     VRFPNFNADYDASVDNLRAAMGADDFEAAWAEGAALSADEAIAYAQRGRGERKRPSTG
                     WGSLTPTEQDVVRLVSEGLPNKDVASRLFISPRTVETHLTHVYAKLALSSRVQLAQEA
                     SRHS"
     gene            3119..3793
                     /locus_tag="VGP27_02560"
     CDS             3119..3793
                     /locus_tag="VGP27_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV7850231.1"
                     /translation="MQVLVVDNYDSFVFNLVQYLGQLGVDAQVWRNDDDRLDTEADIA
                     AAATEFDGVLLSPGPGTPERAGASIPLVKACAAAKTPLLGVCLGHQAIGVAFGGTVDR
                     APELLHGKTSAVSHANIGVLQGLPNPFTATRYHSLTILPDTVPADLEVTARTEGGVIM
                     GVRHEELPIHGVQFHPESILTEGGHRMLANWLAYCGDAPDETLVRTLEDEVANTVRAA
                     TQRSSA"
     gene            complement(3771..>4634)
                     /locus_tag="VGP27_02565"
     CDS             complement(3771..>4634)
                     /locus_tag="VGP27_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV7850232.1"
                     /translation="AVLTVVVTIAINMIGGNTRDVQVPDVGGQLENDATVVLQNAGFK
                     TRTNRQPDSRVLPNRVISTDPGAGSSIGAGDEITLNVSTGPEQREVPDCANLTAAQCV
                     QRLKDAGFGKFKQAPSASTLQQKDKVIGTIPSANQTSAITNEITIVVGSGPEARSVPD
                     VKDQTEDSARQILTASGFTNLVPVPIDNTAPCGQIIGTDPPAGQQVPVDTAIQMQKSA
                     CNQFTMPDLRGQFWTDAEPRLRALGWTGGLDRGADARDSGQRTNAVVTQSPSPGSTVN
                     FSATITLSFAA"
BASE COUNT          756 a         1488 c         1584 g          806 t
ORIGIN      
        1 tggcggtgtg cggccggtcg agcagggtga gggcgacagc ttcgtggtcg cgttcgcgcg
       61 tgccagcgat gctgcggcgt gtgcgctcga attgcagcgg gcgccgctgg cgccgatccg
      121 gctgcgcatc ggcgtgcaca ccggtcagat ccagctgcgc gatgagggca actacgcggg
      181 gccgacgatc aaccgcacgg cccgcctgcg cgatctcggg cacggcggcc agaccctgct
      241 gtctggcgcc accgaaccgt tggtggtcga ctggttgcct gcagacgcct ggctgacgga
      301 actgggtacg catccgctgc gcgacctgcc gcgacccgag cgcgtcgtgc agctgtgcca
      361 ccccgacacc tgcaacgact tcccaccgct gcggacatcg acaaccgtcg acgcgcgcaa
      421 cctgccggtg cagctcacca gcttcatcgg ccgcggggtg cagatggacg acgtgcgccg
      481 gctgctgggc gacaaccggc tggtgaatct caccggcgca ggcggggcgg ggaagacacg
      541 ccttgccgtc gagatagccg cccgcttcgg cggcgacttc cccgacgggg tgcactacgt
      601 cgatctggca cccgtcacgc acgccgaggt cgtgccggtc gcggtcgcgc gagcgctcgg
      661 cctgcccgat cagcccggcc gttcgacggt ggacagcgtg attcggtccg tcggtgagcg
      721 acggatgttg atggtcatgg acaactgcga acacctgttg gagcgcgccg cgttgttgtc
      781 caacgaactc ttgggagcct gcccctcatt gcgactgctc gcgacgagcc gcgaacctct
      841 cggggtaccg ggtgaggtga cttttctcgt gccctcgctg tcgatcggcg acgaggcgat
      901 cgatctgttc accgatcgcg cccgccgcat caggcccgat ttcgctgtca ccgagagcaa
      961 ttcgtcgaaa gtggaggaaa tctgccggcg cctcgacggc atgccgttgg cgatcgagct
     1021 cgcggccgcc cgggtgcggg cgttgtcgtt ggacgacata ctgggcagcc tgcacgaccg
     1081 cttccggcta ctgacgggcg gggcacgcac cgccgtgcgt cgacagcaga ccctgcgcgc
     1141 atcggtcgac tggtcgcacg ccttgctgac cgagtcggag cgtgtgttgt tccgccgatt
     1201 ggcggttttc ctgggcggct tcgacctgga tgccgcgcag gcggtcgggg ggaccaccga
     1261 cgtggagcgc taccaggtgc tcgaccaatt gacactgctc gtggaaaagt cgctggccgt
     1321 cgccgaaaac accagcggcc gaacgcgata ccggctcttg gaaacggtgc gccagtacgc
     1381 acaggagaag ctcggcgagt caggggaggc tgtcgatgtc cgcggccgcc atcgtgacca
     1441 ctatctggcg attgccgacg ttctcaactt gccggcgcga cctgactacc gggactacgt
     1501 cgatcggatt ggcagcgata tcgacaactt gcgcgccgcc ttcgcgtgga gcatggaagc
     1561 gggtgatacg gaggcggcct tgcggctggc gtcgtcactg ttcccgatct ggcaagacgg
     1621 aggacgaaac ggcgagggta tggcatggct gaaggctgct cttacgcagg cagatacggc
     1681 ggctgcatcg gtggatccgg caattcaggc ccgtgcgctg accgacaaag tcatgttggc
     1741 agcgtggacc ctgcgtgtcg cgagcctcga tgaggccgac cgagccctcg cgctggcccg
     1801 cgacaccagc gactcggctt tggcgattcg ggcgttgatc gctcgaggta tctgcaccta
     1861 ctacgacgaa gagctggcgg ggccttgttt taccgaggca gccgaactcg ctcagggctt
     1921 gaatgacccg tggatttgga gccagctcta catggagcaa gcacgttctt caattggcgc
     1981 cggttatcca gcggtcgtcg aggtggcggc cacgaacgcg ctcaaggtat cggccgcaat
     2041 cggaaaccat gccacgaccc gattttgtcg gttcgctcaa gggtgggcac aggcttggag
     2101 gggcgatttt catggtgccc tgatcaaggc tgatgaggct atcggcgaag ccgccgaggc
     2161 acacgacacg atgctgcagc tttatgcact cttggtacag ggattcgtgc gcgcgcagct
     2221 tggcgatgtc gaaggtgcac gcatgtctgc cgaatacgct ctcgaggtag cgaccgactt
     2281 gaaggcgttc tatgtggcgc ccggtcaagc ttccatcgcg gtggtccatc aagctgcagg
     2341 cgacgcggaa gcagcccaac gcgcgtacgc cgtggcgcga gaggacgccg ggctcaaccg
     2401 aatggccgcc ggcgccttct gtttctccgc tcttgcgccg ctcgcttgcg gtgatcgcca
     2461 taccgcgcgg gaatgggccg aagtagacgt agaggtgtca tatggctgct accagtcggc
     2521 ggctctgatg actcgcgctc aagtgaagct cgtcgacggc gaacccgagg gcgcgcagga
     2581 cgatgcggat gcggcgctgt caatcgcgac ggaggtcggg atagcgcacc ggatgacgat
     2641 ggcttttgag gttctcgcga cactggccac gagggcggaa agccaccgcg aagcggcccg
     2701 gcttttcggt gcggcggagg ccatgcgtca acgcaccggc gaagtccgtt tcccgaactt
     2761 caacgccgac tacgacgcga gcgtcgacaa cctacgagcc gcgatgggcg ccgacgactt
     2821 cgaggcggcc tgggccgaag gtgccgccct gtcggccgac gaggcgatcg cttacgcgca
     2881 acgcgggcga ggcgagcgca agcggccgtc gaccggctgg ggatcgctga cgcccaccga
     2941 acaggatgtg gtccggttgg tcagcgaggg gctgcccaac aaggacgtcg cgtcgcggct
     3001 gttcatctca ccgcgcacgg tggagaccca cctcacccac gtgtacgcca agctcgccct
     3061 gtcgtcgcgg gtccaactcg cccaggaagc ttcacggcat agctagtaac ctgggctgat
     3121 gcaggtcttg gtcgtcgaca actacgacag cttcgttttc aacctggtcc agtacctggg
     3181 ccagctcggt gtggacgcgc aggtatggcg taacgacgac gaccgcctcg acaccgaggc
     3241 cgacatcgcc gcggccgcca cggagttcga cggcgtgctc ctcagccccg gacccggtac
     3301 gcccgaacgc gccggcgcat ccattccgct cgtcaaggcc tgcgctgcgg ccaagacgcc
     3361 gctgctcggc gtgtgcctgg gtcaccaggc gatcggggtg gcgttcggcg gcaccgtgga
     3421 ccgggcgccg gagctgctgc acggaaagac gagcgcggta tcgcatgcga atatcggtgt
     3481 gttgcagggc cttccgaacc ctttcacggc cacgcggtat cactcgctga ccattctgcc
     3541 cgacaccgtg cccgccgacc tcgaggtcac cgcccgcacc gagggcgggg tcatcatggg
     3601 tgtgcggcac gaagaactgc ccatccacgg agttcagttc caccccgagt cgattctcac
     3661 cgagggcggc caccggatgt tggccaactg gctggcctac tgcggagacg ctccggacga
     3721 gaccctggtg cgcacactgg aggacgaggt cgccaacacg gtgcgcgcgg ctacgcagcg
     3781 aagctcagcg taatcgtcgc gctgaaattc accgtgctgc ccggcgacgg actctgagtg
     3841 accactgcgt tggttcgctg gccgctgtcg cgcgcatcgg cgcccctgtc gagcccgccg
     3901 gtccagccca gcgcgcgcag ccgcggctcg gcgtcggtcc agaactggcc ccgaaggtcg
     3961 ggcatcgtga actggttgca cgccgacttc tgcatctgaa tcgccgtgtc gaccggaacc
     4021 tgttgtcccg caggcgggtc agtgccgata atctgtccgc acggcgcggt gttgtcgatg
     4081 ggaaccggga cgaggttggt gaaccccgac gccgtcagga tctgccgagc cgaatcctcg
     4141 gtctggtcct tgacgtcggg caccgacctg gcttcgggtc cggaaccgac gacgatggtg
     4201 atctcgttgg tgatggccga agtctgattc gccgacggga tggtgccgat caccttgtcc
     4261 ttctgctgga gtgtggacgc tgacggagcc tgcttgaatt ttccgaagcc ggcgtccttg
     4321 agtctctgga cacactgggc ggccgtgagg ttcgcgcagt ccgggacctc acgctgttcc
     4381 ggaccggtgg acacgttgag ggtgatctcg tcgcccgcgc cgatagacga accagcgcca
     4441 gggtccgtac tgataacccg gttcgggagg accctggagt cgggctggcg gttggtccgg
     4501 gtcttgaagc cggcgttctg cagcacgacg gtcgcgtcgt tctccaactg cccgccgacg
     4561 tcgggcacct gcacgtcgcg ggtgttgccg ccgatcatgt tgatcgcgat cgtcaccacc
     4621 accgtgagca cggc
//
LOCUS       DASYLX010000106         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2966929,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000106 DASYLX010000000
VERSION     DASYLX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2966929"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VGP27_02570"
     CDS             complement(<1..105)
                     /locus_tag="VGP27_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV7850233.1"
                     /translation="MRSTPRCWLTDMDGVLVREEHALPGAAEFLHRLVE"
     gene            262..1452
                     /locus_tag="VGP27_02575"
     CDS             262..1452
                     /locus_tag="VGP27_02575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7850234.1"
                     /translation="MALGGDTGRVPPGLTLDNWQSAAQLHWTFQHVAEFLPTAVISRG
                     NGPVAELPSAPVDLVDIPLYDRTNARRTTVGEVMAATDTDGWIVTRHGKVLGEFYYGG
                     MQADSSHILMSVSKSLVAMVLGALVTNGAVNPDAELTYYVPALANSGYAGATVRHLLD
                     MRSGIKFSEEYLDPLAEVRLIEQAIGWAPRTVPDLPETMYDFLVTLQQKSPHGGPFEY
                     RSCETDVLGWICEAAGGLRMPELMSQVLWARLGARNDATIGVDSVGTGFFDGGINAGL
                     SDLVRFGSLFLNGGTSPTGERVVSLSWIADTFAGGLDSRAAFAASRGDSGMPGGMYRN
                     QVWFPYPGNNVLLCIGIHGQMIYVNRAADMVAAKLSSWPLPQDATKLFPTIAAFDAIA
                     SHLS"
     gene            1469..>2011
                     /locus_tag="VGP27_02580"
     CDS             1469..>2011
                     /locus_tag="VGP27_02580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7850235.1"
                     /translation="MQTITIDAPGGPIDALLSVPDGAGPWPGVVLVHDAIGYGPDNER
                     ISRLVAAAGYIAVTPNMFARGGRARCITRVFRDLLTKRGRALDDILAARDHLLGLPQA
                     SGAVGIAGFCMGGQFALLMSPKGFGASAPFYGTPLPRNLSETLDGACPIVASFGGRDP
                     VGIGAAPKLQKVVDEKDIDAD"
BASE COUNT          307 a          689 c          669 g          346 t
ORIGIN      
        1 ctcgacgagc cggtgcagga actcggcggc gcccggaagg gcgtgctcct cgcggaccag
       61 cacgccgtcc atgtcggtca accagcaccg gggcgtcgat cgcacgcgct cagtctccca
      121 gttttatccg cgggttctcg gcctttacga cgatactggt tacccgtgtc acgaaacgtt
      181 gacattgccc ccggtttacg gccgcgctca tcgccggcta cgcggagtcg ccgacaggcg
      241 gcggccacta cagtcgtcgc catggctctc ggaggcgaca ccggacgcgt gccgccgggc
      301 ctgaccctgg acaactggca gtcggcggcc cagctgcatt ggacgtttca gcacgtcgcc
      361 gagttcctcc cgaccgctgt gatctcgcgc ggtaacggac ccgtcgccga gctgccgtcg
      421 gctccggtcg atctggtgga catcccgctg tacgaccgga cgaacgccag gcgcaccacc
      481 gtcggcgagg tgatggccgc gaccgacacc gacggctgga tcgtcacccg gcacggcaag
      541 gtgctcggcg agttctacta cggcggaatg caggccgact cgtcgcacat cctgatgtcg
      601 gtgagcaagt cgttggtcgc catggtgctc ggtgcgcttg tcaccaacgg cgcggtgaac
      661 cccgacgccg aactgactta ctacgtgcct gcgctggcca actccggcta tgcgggcgcg
      721 actgtgcgac acctgctcga catgcgttcg ggcataaagt tttccgagga gtatctcgat
      781 ccgttggccg aagtgcggtt gatcgagcag gccatcgggt gggcgcctcg cacagtgccg
      841 gaccttccgg agacgatgta cgacttcctg gtgacccttc agcagaaatc gccgcacggc
      901 ggcccgttcg agtaccggtc ctgcgagacc gatgtgctcg gctggatctg cgaggcggcc
      961 ggcgggctgc ggatgccgga gctgatgtcg caggtgctgt gggccaggct gggtgcgcgc
     1021 aacgacgcga ccatcggcgt cgactcggtg ggcacgggct tcttcgacgg cggcatcaac
     1081 gccggcctgt cggatctggt gaggttcggt tcgctgttcc tcaacggcgg aacgtcgccg
     1141 acgggtgagc gggtggtgtc gctgtcatgg atcgccgaca ccttcgcggg cgggctcgac
     1201 tcgcgggccg ccttcgccgc gagccgcggt gacagtggca tgcccggtgg catgtaccgc
     1261 aaccaggtct ggttccccta tccaggcaac aacgtcttgc tgtgcatagg gattcacggt
     1321 cagatgatct atgtcaaccg ggcagcggac atggtcgccg cgaagctgtc gagctggccg
     1381 cttccgcagg acgccacgaa gctgttcccc accatcgccg ccttcgacgc catcgcctcg
     1441 cacttgtcgt gaccgctaga ttgatcgcgt gcagaccatc acgatcgatg cccccggcgg
     1501 tccgatcgac gcgctgctca gcgtgcccga cggcgcaggc ccgtggccgg gtgtggtgct
     1561 cgtccacgac gcgatcggct acggcccgga caacgaacga atctcgcgcc tcgtcgccgc
     1621 ggcgggatac atcgccgtca cgccgaacat gttcgcccgc ggcgggcgcg cccgctgcat
     1681 cacccgtgtc ttccgtgacc tgctgaccaa acgcggccgt gcgctcgacg acatcctcgc
     1741 cgcccgcgac cacctgctcg gcctgccgca ggcctcgggc gctgtcggca tcgcaggctt
     1801 ctgcatgggc ggccagttcg cgctcctgat gtcgcccaaa ggatttggcg catcagcgcc
     1861 gttctacggg accccgctgc cgagaaacct cagcgagacg cttgacggcg cctgcccgat
     1921 cgtggcgagc ttcggcggcc gcgacccggt cggtatcggc gcggcgccga agctgcagaa
     1981 ggtcgtcgac gagaaggaca tcgacgccga c
//
LOCUS       DASYLX010000107         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3186314,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000107 DASYLX010000000
VERSION     DASYLX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186314"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3488)
                     /locus_tag="VGP27_02585"
     CDS             complement(<1..>3488)
                     /locus_tag="VGP27_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV7850236.1"
                     /translation="AGGLGVERFVEIGVKSAPTVAGLATNTLKLPEYSHSTTEVLNSE
                     RDAAVLFATDTDPAPDHDEDVDDEPVPAQEAAAEPVEAPAAAPAAATAPAGAPQPDDI
                     PFDASDATMALIALSAKMRIDQIEALDSIESITDGASSRRNQLLVDLGSELDLGAIDG
                     AAEADLAGLKGQVTKLARTYKPYGPVLSDAINDQLRTVLGPSGKRPAAIAERVKKTWM
                     LGDGWVKHVTAEVALGTREGSSVRGGGLGGLHDGALADVATVDKVVDAAVQSVAARRG
                     VAVALPSAGGGAGGGVVDSTALNEFAEQVTGRDGVLASAARLMLNQLGLDNAVTTPEA
                     TSDAELIDLVTAELGSDWPRLVAPVFDGRKAVLFDDRWASAREDLVKLWLMDEGEIDA
                     NWQKLSQRFEGAGHVVGKQASWWQGRSLAAGRNIHASLFGRAAAGAENPGKGRYSGEI
                     AVVTGASNGSIAASVAAGLLDGGATVIATTSKLDDNRLEFYRNLYRDNARFNAALWVV
                     PANMASYSDIDALVAWVGNEQSENLGPKSVHVKDAQTPTLLFPFAAPRVAGDLSEAGS
                     RSEMEMKVLLWAVQRLIGGLSHIGSERDIASRLHVVLPGSPNRGMFGGDGAYGESKSA
                     LDAVVTRWNAESSWAQRVSLAHALIGWTRGTGLMGANDAIVSAVEEAGVTTYSTEQMA
                     AMLLALCNIESKVAAAQAPLKVDLTGGLGDIDIDMSELAAKAREEMGQKDQAAASSES
                     ENGTIPALPSPPRGFAAVPAPAWDDLDIDPADMVVIVGGAELGPYGSSRTRFEMEVDN
                     ELSAAGVLELAWTTGLIAWEDDPKPGWYDTESGDLVDESELVERYHDAVVERTGIREF
                     VDDGAIDPDHASPLLVSVFLDKDFTFVVSSEADARAFVQFDPEHTVAVPVPDSSDWQV
                     TRKAGTEIRVPRKTKLSRTVGGQIPTGFDPTVYGISADMNTSLDRVAVWNIVATVDAF
                     LGAGFNPTELMRWVHPTMVASTQGTGMGGMTSMQTMYHGNLLGRNKPNDILQEVLPNV
                     VAAHVIQSYVGSYGSMIHPVGACATAAVSVEEGVDKIKLGKAELVVAGGFDDLTLEAI
                     IGFGDMAATADTETMRARGISDSKFSRANDRRRLGFLESQGGGTILLARGDLALKMGL
                     PVLAVVGYAQSFADGV"
BASE COUNT          550 a         1235 c         1136 g          567 t
ORIGIN      
        1 cacaccgtcg gcgaacgact gcgcgtagcc caccacggcc agcaccggca gacccatctt
       61 cagcgcgagg tcaccacgcg ccagcaggat cgtgccgccg ccctgcgact ccaaaaagcc
      121 cagccggcga cggtcgttgg cgcgggagaa cttcgagtcg ctgatgcccc tggcgcgcat
      181 cgtctcggtg tcggcggtgg ccgccatgtc accgaatcct atgatcgctt cgagcgtcag
      241 gtcgtcgaaa ccgcctgcga ccacgagctc ggccttgccc agtttgatct tgtcgacgcc
      301 ctcttccacc gagaccgccg ccgtcgcgca ggcgccgacc gggtggatca tcgagccgta
      361 gctgccgacg taggactgga tcacgtgtgc ggcaacgacg ttcggcagga cctcctgcag
      421 gatgtcgttc ggcttgttcc gccccagcag gttgccgtgg tacatggtct gcatcgacgt
      481 catgccgccc atgccggtgc cctgcgtgct ggccaccatc gtggggtgca cccagcgcat
      541 cagctcggtc ggattgaatc ccgcgccgag gaacgcatcg acggtcgcga cgatgttcca
      601 caccgccacc cggtccagcg acgtgttcat gtcagcgctg atgccgtaca ccgtcgggtc
      661 gaagccggtc ggaatctgac cgccgacggt ccgcgacagc ttcgtcttgc gcggaacccg
      721 aatctcggtg cccgccttgc gggtgacctg ccagtcgctg ctgtccggta ccggcaccgc
      781 aacggtgtgc tccgggtcga actgcacgaa cgcgcgggcg tcggcctccg aggacaccac
      841 gaaggtgaag tccttgtcga ggaacaccga caccagaagc ggtgacgcgt ggtcggggtc
      901 gatcgccccg tcgtcgacga actcgcggat accggtccgc tccacgacgg cgtcgtgata
      961 gcgctcgacc aattcggatt cgtcgaccag gtcgccggat tcggtgtcat accaaccagg
     1021 cttcgggtcg tcctcccacg cgatcaggcc ggtggtccag gccagctcga gcaccccggc
     1081 ggccgacagt tcgttgtcga cctccatctc gaagcgggtc cgcgatgagc cgtacggtcc
     1141 cagctcggcg ccaccgacga tcaccaccat gtcggcaggg tcgatgtcga ggtcgtccca
     1201 cgcaggcgcg ggcacggccg cgaagccacg cggcggcgac ggcagcgcgg gaatcgtgcc
     1261 gttctcggat tcggaggaag cggccgcctg gtccttttga cccatctcct cacgggcctt
     1321 ggcggccagt tcggacatgt cgatgtcgat atcgccgagc ccaccggtca ggtcgacctt
     1381 cagcggcgcc tgcgcggcgg ccaccttcga ctcgatgttg cacagcgcga gcagcatggc
     1441 agccatctgc tcggtcgagt aggtggtgac acccgcctcc tcgacggcac tgacgatcgc
     1501 gtcgttggcg cccatcaagc cggtgcctcg ggtccagcca atcagtgcgt gcgccaggct
     1561 gacccgctgc gcccacgacg actcggcgtt ccaccgggtg acgaccgcat cgagcgcgga
     1621 cttcgattcg ccgtaggcgc cgtcgccgcc gaacatgccg cggttcggcg aaccgggcag
     1681 caccacatgc agccgcgacg cgatgtcgcg ctcggaaccg atgtgggaca ggccgccaat
     1741 caaccgctgc accgcccaca gcagcacctt catctccatc tcggagcgag agcctgcctc
     1801 ggacagatcg cccgccaccc gcggtgccgc gaacgggaac agcagggtcg gcgtctgtgc
     1861 atccttgacg tgaacggact tcggcccaag gttttcggat tgctcgttgc cgacccacgc
     1921 caccagcgcg tcgatgtcgg agtaggacgc catgttggca ggcaccaccc acagcgccgc
     1981 attgaatcgg gcgttgtcgc ggtacagatt gcggtagaac tccagccggt tgtcgtcgag
     2041 tttggacgtg gtcgcgatga cggtcgcgcc gccgtcgagc agtccggccg ccaccgaagc
     2101 ggcgatcgac ccgttcgaag cacctgtcac gacagcgatt tcgccgctgt agcgaccctt
     2161 gcccgggttt tccgcacctg ctgcggcgcg gccgaacagc gacgcgtgga tgttgcgtcc
     2221 ggcagccaga gacctgccct gccaccacga ggcctgcttg ccgacgacgt ggccggcgcc
     2281 ctcgaagcgc tgcgagagct tctgccagtt ggcgtcgatc tcgccctcgt ccatcagcca
     2341 gagcttgacc aggtcttcgc gcgcgcttgc ccaccgatcg tcgaacagca ccgccttgcg
     2401 gccgtcgaac accggcgcca ccaaacgtgg ccagtccgaa ccgagttcgg cggtgaccag
     2461 gtcgatcaac tcggcgtcgc tcgtcgcctc gggagtggtg accgcgttgt ccagcccaag
     2521 ctggttcaac atcaggcggg cggccgaagc cagcacgccg tcacgaccgg tgacctgctc
     2581 ggcgaactcg ttgagcgccg tcgagtccac gacaccgccg ccggcgccgc cacccgctga
     2641 gggcagcgcc accgcgacac cgcgtcgggc cgccaccgac tgcacggcgg catcgacgac
     2701 cttgtcgacg gtcgcgacgt cggccagtgc gccgtcatgc aacccgccga ggccgccacc
     2761 gcggacgctg gaaccctcgc gggtgcccag cgcgacctcc gccgtgacgt gcttgaccca
     2821 gccgtcgccg agcatccagg tcttcttgac ccgctcggcg atcgcggccg gacgcttgcc
     2881 ggacgggccg agcactgtgc gcagctggtc gttgatcgcg tcggaaagca caggaccgta
     2941 aggcttgtag gtccgggcaa gcttcgtcac ctggcctttg agcccggcca gatcggcttc
     3001 ggcggcaccg tcgatggcgc cgaggtccag ctccgagccg agatcgacca acagctggtt
     3061 acgtcgagac gacgcaccgt cggtgatcga ctcgatggag tccagcgcct cgatctgatc
     3121 gatgcgcatc ttcgccgaca gcgcgatcag cgccatcgtc gcatcggacg cgtcgaacgg
     3181 aatgtcgtcg ggttgcggag cgccggcggg cgcggttgca gcagccggtg ccgcagccgg
     3241 tgcctcgacg ggttcggcgg ctgcctcctg cgcgggcacc ggctcgtcgt cgacgtcctc
     3301 gtcgtgatcg ggcgcgggat cggtgtcggt cgcgaacaat accgcggcgt cgcgctcgga
     3361 attgagcact tccgttgtgc tgtgcgagta ctcgggcagc ttgagcgtgt tggtggccag
     3421 cccggcaacg gtcggtgcgg acttcacgcc gatctcgacg aaccgctcga ccccgagacc
     3481 gcctgccg
//
LOCUS       DASYLX010000108         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2637614,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000108 DASYLX010000000
VERSION     DASYLX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637614"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1439
                     /gene="fucP"
                     /locus_tag="VGP27_02590"
     CDS             123..1439
                     /gene="fucP"
                     /locus_tag="VGP27_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018019227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEV7850237.1"
                     /translation="MTTTEHAEQPAATAKTGLVYPGMGVLFVVLICCFTAWGIAADLT
                     TPMVAGFKAIFDMNTFQASLVQLAYFGAYFLLAIPAAFINQRFGYKAGLLSGVLLAAA
                     GAIAFYPASKIMTYEAFLVALFAIAAGCSILETSANPYVLSLGPEETATRRLNFAQAF
                     NPVGTNIGVLLAATLILPKLDQPVNMANLTPAQEQTIRAGQLGAVMGPYLGLGFVLIA
                     IGLVIAIKKSPPIVEEFEAGDLEGKRPLKILLSNKRYVYGVIAQFFNVAAQVCTWTYL
                     IQYSQQALNGSLELGGYLLQVSLIVFLISRFIMTWVIGRIRATKVLAVLGALAVVLCI
                     FAMFSPNVAGVAAVVALSFCLSLMFPTIYGVALQGLGPATKFGAAGLVMAIVGGAIMP
                     LIQGRLLDATSPATSFVVPAICFAVVTSFAVFDLTAAPHKVGEPAS"
     gene            1436..2707
                     /locus_tag="VGP27_02595"
     CDS             1436..2707
                     /locus_tag="VGP27_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7850238.1"
                     /translation="MRRYWIIGMAFILVACGGGAQNANFSEPTKRLEVVSWWVSPSEH
                     PALEVLLNAFKARNPDVEVVDGAIAGGGGSNVQVALAARLRAGDPPDVWQTFLGSSLR
                     AWVDAGHITDVSGVYEGSGLDRTMPQTLLEAATYRVKAWGVPTGSHRGNVLWFNQRML
                     RDAGVAAPGAGYTIEAFEADLAKVAASGKTALCLGSKDRFTATELFENTLLGVAGTDG
                     WARIRDDSFDWRGSQLRQALSQFAKIVSLADPNAGGLTWDQAAKKLATGQCAFLSMND
                     SVYGELVANRSVEGKDFGYVPYPGTTGAYLAIVDTFVVSSDAKDGVNAMKFMETIADP
                     KTSLEFNKAKGSVPIRNDVDVSSLPVYQRQASESLWKDRILLSITHGELMSTDFQQAV
                     YDAVAAFVQSKNTDAFIDTLQNSIEVPIAGR"
     gene            2694..3002
                     /locus_tag="VGP27_02600"
     CDS             2694..3002
                     /locus_tag="VGP27_02600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7850239.1"
                     /translation="MPDDDGDGPLFLVVTIKPRLDRLREAEAQLQSMRQNTLSEPGCQ
                     FMYFLQPHDNSDTWVMLEMFRSRAAWDEHMRQPYNTEGNKVLEDLLREPSDLRLLREK
                     "
     gene            3028..>3963
                     /locus_tag="VGP27_02605"
     CDS             3028..>3963
                     /locus_tag="VGP27_02605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEV7850240.1"
                     /translation="MIELNEGTLATLPSNVATPTYDRTQLKRGIAHFGVGNFHRAHQA
                     FYVDRCLALPGQQDWGIVGIGLSSGERGRKKADQFRSQDCLYSLTVAPAKGDASVRII
                     GAQLDYLLAPEQPQEVLELLTDPALRIVTLTITEGGYHVDAGSGAFVTEHPDVAHDLA
                     GEGTPRTVFGFVTAALARRRSAGTKPFTVVSCDNLRHNGEVARAAFVGFASALDPELG
                     AWIDSNVTFPNSLVDRITPSVSAEDAARLNAASGLADQIPVVAEEFTQWVIEDRFVDG
                     RPALHEVGVQFSDEVKLWEQVKVRVLNAGHLTLTYP"
BASE COUNT          764 a         1222 c         1195 g          782 t
ORIGIN      
        1 cgaaaaccca cgtcaagcca catgcgcaat ctgttccatc ggtcaggaca tatgagtaac
       61 gtgacctaga tcacagtcta atcggggctg aatttcttct cagcggacga gaggtactga
      121 tcatgacgac caccgagcac gccgaacagc ccgccgcgac agcaaaaact gggttggtct
      181 accccgggat gggggtgctg ttcgtagttc tcatctgttg tttcactgcg tggggcatcg
      241 ccgccgacct caccacaccg atggtcgcgg ggttcaaggc gatcttcgac atgaacacgt
      301 tccaagcttc gctggtgcaa ttggcgtact tcggcgcata tttcctgctg gccatcccgg
      361 cggcgttcat caaccagcga ttcggataca aggccggcct cctctccggt gtgctgctcg
      421 ccgctgccgg cgcgattgcg ttctatccgg ccagcaagat catgacctat gaggcgttcc
      481 tggtagcgct cttcgcgatc gccgccggat gctcgatcct cgagacatcg gccaacccgt
      541 acgtgctttc gctcggaccg gaggaaaccg cgacccggcg gctcaacttc gcgcaggcct
      601 tcaaccccgt cggcacgaac atcggcgtgc tcttggccgc caccctcatc ctgccgaagt
      661 tggaccaacc ggtgaacatg gccaacctga caccggcgca ggaacagaca atccgcgccg
      721 gccagctcgg cgcagtgatg ggcccatacc tcggactcgg attcgtcctc atcgccatcg
      781 gcctggtgat cgccatcaag aagtcgccac cgatcgtcga ggagttcgag gcgggagatc
      841 tggaggggaa gcgaccgctg aagatcctct tgtcaaacaa gcgctatgtc tacggtgtga
      901 tcgcgcagtt cttcaacgtg gcggcgcagg tttgtacgtg gacgtacctg atccagtact
      961 cgcaacaggc cctcaatggg tctttggagc tgggtggata tctcctgcag gttagtttga
     1021 tcgtgtttct catctcccgg ttcatcatga cgtgggtaat cgggcggatc cgcgccacaa
     1081 aggtgctcgc ggtgctcggc gcattagcgg tcgttctgtg catattcgcg atgttctcgc
     1141 cgaacgtggc gggagtggcc gccgtcgtcg cgttgtcctt ctgcctgtcg ttgatgttcc
     1201 cgacgatcta cggtgttgca cttcaaggtc ttggtcccgc aaccaaattc ggcgcagctg
     1261 gactggtgat ggcgatcgtc ggcggcgcga tcatgccgct gatccaggga cgcctcctcg
     1321 acgcgaccag cccggcgact tcgttcgtcg tgcccgcgat ctgcttcgcg gtagtcacca
     1381 gttttgccgt cttcgacctg acggcggccc cgcacaaggt tggagagccc gcatcatgag
     1441 gcggtactgg atcatcggaa tggcgttcat cctcgtggca tgcggtgggg gcgcacagaa
     1501 cgcgaatttc tccgaaccga ccaaacgact ggaagtggtc tcctggtggg tgtcgccttc
     1561 ggaacaccca gcattagagg tgctgctcaa cgcattcaag gcgcggaatc ccgatgtaga
     1621 ggtggtcgac ggcgcaatcg ccggcggtgg cggtagcaac gttcaggttg cactcgcggc
     1681 acgactgcgg gcgggtgatc cgcctgacgt atggcaaacg ttccttggca gttcgctacg
     1741 tgcttgggtc gatgccggcc acatcaccga cgtatcgggc gtctacgaag gctcgggact
     1801 ggaccgcacc atgccgcaga cattgctcga ggcggccacc tatcgggtca aagcctgggg
     1861 cgtgccgact ggatcccatc gcggaaacgt gttgtggttc aaccagcgga tgctgcgtga
     1921 cgccggcgtg gcggcgcccg gggccgggta cacgatcgag gcattcgagg cagacctcgc
     1981 aaaggtggcg gcaagcggaa agaccgcgct gtgcctcggc agcaaggaca ggttcaccgc
     2041 aaccgaactg ttcgagaaca cgctgctcgg tgtggccggc accgacggat gggccaggat
     2101 tcgggacgat tcgttcgact ggcggggatc tcaacttcgg caagcgcttt cgcaattcgc
     2161 aaagatcgtc tcgctcgccg atcctaacgc cggcggcctg acttgggatc aggcggcgaa
     2221 gaaacttgcc accggtcagt gcgccttcct gtcgatgaac gactccgtct atggcgaact
     2281 cgtggcgaac cggtcggtcg agggcaagga cttcggctat gtgccctatc ccgggaccac
     2341 cggcgcgtat ttggcgatcg tggacacgtt cgtggtttcg tccgatgcga aggacggcgt
     2401 caacgccatg aaattcatgg agacgatcgc ggacccgaag acatcgctgg aattcaataa
     2461 agcgaaaggg tctgtgccga ttagaaatga cgtcgacgtg tcgtcactgc ccgtctatca
     2521 gcgacaagct tcggagtcgt tgtggaaaga caggatactg ctgtcaatca cccacggcga
     2581 gttgatgtcg acggacttcc aacaggccgt gtacgacgcg gtcgccgcat tcgtccaaag
     2641 caagaatacg gacgcattca tcgacacatt gcagaactcg atcgaggtgc caattgccgg
     2701 acgatgacgg cgacggaccg ttatttctcg tcgtgacgat caagccccga ctggaccgcc
     2761 tccgggaggc cgaggcccaa ctacagtcca tgcgccagaa cacgctgagc gagccaggct
     2821 gtcaatttat gtatttccta cagcctcacg ataattcgga cacgtgggtg atgctcgaga
     2881 tgttccgctc gcgcgccgcc tgggacgagc acatgcgtca gccctacaac accgaaggca
     2941 acaaggttct cgaggacctc ttgcgcgaac cctctgacct tcgactgctc cgcgagaagt
     3001 aagcactgtt tgtttggagg gtcaaccatg atcgaactca acgagggaac gctggcgaca
     3061 ctcccgtcaa acgtcgcgac accgacctat gaccgaaccc aactcaaacg cggtatcgcc
     3121 catttcggcg tcggcaactt ccatcgggca catcaagcct tctacgtcga ccgctgtctt
     3181 gcgctacccg gacagcagga ttggggcatt gtcggcatcg gcctttcgag cggcgaacgg
     3241 ggccgcaaga aggccgatca gttccgttct caggattgtc tgtactcgct caccgttgcg
     3301 ccggccaagg gcgacgcgag tgtgcgcatc atcggcgctc agctcgatta cctgctcgcc
     3361 cccgagcagc cgcaagaggt gctggagttg ttgaccgacc cggcccttcg catcgtgacg
     3421 ctgaccatta ccgagggcgg ttatcacgtc gacgcaggct cgggtgcgtt cgtcaccgaa
     3481 caccctgacg tggcgcacga cttggccgga gaaggcactc cgcgcacggt gttcggattt
     3541 gtcaccgcgg cactggcgcg gcgccgttca gccggcacca aaccgttcac cgtggtgtcc
     3601 tgtgacaacc tgcgccacaa cggagaagtg gcccgcgcgg cctttgtcgg cttcgccagc
     3661 gcgctcgacc cagaactcgg tgcatggatc gattccaacg tcacctttcc taactctctt
     3721 gtcgaccgca tcacgccctc agtgtcggcc gaggacgcag cccgcctcaa tgccgcaagc
     3781 ggtctggccg accaaattcc agtcgttgct gaggaattca cccagtgggt catcgaagac
     3841 cgcttcgtcg acggccgccc ggcacttcac gaagtgggcg tgcaattcag cgacgaagtc
     3901 aagttatggg agcaggtgaa ggtgcgtgtg ctcaacgccg gccacctcac gctgacctat
     3961 ccc
//
LOCUS       DASYLX010000109         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_115101,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000109 DASYLX010000000
VERSION     DASYLX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115101"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /locus_tag="VGP27_02610"
     CDS             complement(<1..2103)
                     /locus_tag="VGP27_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7850241.1"
                     /translation="MMSEEWQSTACILCECNCGIVVQLEGRTLHKIRGDKEHPASQGY
                     TCNKALRLDHYQNNRSRLTSPMRRRPDGSYEEIDWDTAITEIAEGFKRIRDTYGGDKV
                     FYYGGGGQGNHLGGAYSGAFLKAIGARYRSNALAQEKTGEAWVDAHLYGGHTRGEFEH
                     AEVSVFIGKNPWMSQSFPRARTVLNEIAKDPARTMIVIDPVITDTAKMADIHLRVRPG
                     TDAWCLAAMAAVLVQENLCDEGFLAEHVNGADAVRELLRQVPVADYAERCGVDEELIR
                     NAVRRIAAADGAAVFEDLGIQQAPNSTLCSYLNKLLWILTGNFAKRGGQHLHSAFAPL
                     FTFGGVGRTPVTGAPVISGLVPSNVVPQEILTDHPDRFRAMIVESSNPAHSVADSAAC
                     KEAFESLELMVVIDVAMTETARLAHYVLPAASQFEKPEATFFNLEFPHNTFQLRHPLL
                     EPLPGTLPEPEIWARLVRALGVVEDADLKPLREAADQGLDAYAQAFLGAVSANPAMSR
                     VLPYVLYETLGPTLPEHLKGAAALWGLAQRTAMTYPEAVRRAGHADGNALFEAILKGR
                     SGVTFTVHQYEDDFGLIAHADHKIALEMPEMLDEIRGLRDARSALTTLEFPVVLSAGE
                     RRAYTANDIFRDPSWRKRDGEGALRVSVEDAQSLGLVAGGRARITTAAGSAEASVEIS
                     EAMLPGHASLPNGLGLDFV"
     gene            complement(2100..2663)
                     /locus_tag="VGP27_02615"
     CDS             complement(2100..2663)
                     /locus_tag="VGP27_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850242.1"
                     /translation="MVVSAALLIRERGAQATAISDVLKHSGAPRGSAYHYFPGGRMQL
                     LEEATDYAGAYIADKISAAESAVELLDDLTELYRRQLIRSDFRAGCPVVAVTVEAGDP
                     DNPERATKVIERAAAAFAMWTDLIGQLLIGEGVSAGRAEELAMLVTTSIEGAIIVARA
                     SRDVTPLDLVQRQLRTLLEAETAERKS"
     gene            complement(2810..>3062)
                     /locus_tag="VGP27_02620"
     CDS             complement(2810..>3062)
                     /locus_tag="VGP27_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850243.1"
                     /translation="EIVAEPFLIVGILAGIKEIVVLSVEAATLLDKGPAFARAVVEIG
                     VLAGVVLALALAAFVLRERRRDTQDVGEDCADEKQPSKT"
BASE COUNT          513 a         1010 c          985 g          554 t
ORIGIN      
        1 gacgaaatca aggccgagcc cgttcggcaa cgacgcgtgg ccgggaagca tcgcctcgct
       61 gatctcgacg ctcgcctcgg cgctgcccgc cgcggtggtg atgcgggcgc gtccacctgc
      121 gaccagaccg agtgactgcg catcctccac gctgacgcgt aacgctccct ctccgtcgcg
      181 cttgcgccag ctggggtcgc ggaagatgtc gttggccgtg taggcacgtc gctcaccagc
      241 ggacagcacg acggggaact cgagcgtcgt cagcgcagac cgcgcatccc gcaatccgcg
      301 tatctcatcg agcatttcgg gcatctccaa cgcgatcttg tgatcggcgt gggcgatcag
      361 cccgaagtcg tcctcgtatt ggtgcaccgt gaacgtcaca ccggaacggc ctttcagtat
      421 cgcctcgaag agcgcgttgc cgtcggcgtg accagcgcgt cgcacggctt cgggatacgt
      481 catcgccgtc ctctgcgcga gaccccacag ggccgcggcg cccttgagat gctccggcag
      541 cgtgggaccc aacgtctcgt agaggacgta gggcagcacc ctcgacatgg cgggattggc
      601 gctgaccgct ccgaggaacg cctgggcata cgcatcaaga ccctgatcgg cggcctcgcg
      661 caatggcttg aggtcggcgt cctcgacgac acccagcgcc cgcaccaggc gtgcccagat
      721 ctctggctcg ggcaacgtgc cgggtaaggg ctcgaggagg ggatgccgaa gctggaatgt
      781 gttgtgcggg aactcaaggt tgaagaaggt cgcttcgggc ttctcgaact ggctcgctgc
      841 gggcaacacg tagtgggcca gccgtgccgt ctcggtcatc gcgacatcga tgaccaccat
      901 cagttccagc gactcgaacg cctctttgca cgccgcggag tcggcgaccg agtgggcggg
      961 gttgctgctc tcgacgatca tcgcccggaa ccggtcggga tggtcggtca ggatctcctg
     1021 cggcacaacg ttggacggca ccagcccgga gatgaccggc gcgcccgtga cgggtgtccg
     1081 tccgacgccg ccgaacgtga acagcggcgc gaacgccgaa tgcagatgct gaccaccgcg
     1141 tttggcgaag ttgccggtga ggatccacag cagcttgttc agatacgaac acagcgtgct
     1201 gttgggcgcc tgctggatgc cgaggtcctc gaagaccgct gcgccgtctg cggcggcgat
     1261 gcgccgcact gcgttgcgga tcaactcctc gtccacccca cagcgctcgg cgtagtcggc
     1321 gaccgggact tgtcgcagca actcgcggac ggcgtctgcg ccgttgacgt gctcggccag
     1381 aaacccttcg tcgcagagat tttcctgcac cagcaccgcg gccatcgccg ccaggcacca
     1441 cgcgtctgtg ccgggccgta ctcgcagatg gatgtcggcc atcttggccg tatccgtgat
     1501 caccggatcg atgacgatca tcgtgcgggc agggtccttg gcgatttcgt tgagcacggt
     1561 gcgggcgcgc gggaaactct gcgacatcca cgggttcttg ccgatgaaga ccgaaacctc
     1621 ggcgtgctcg aactcgccgc gggtatgccc gccgtagagg tgcgcgtcga cccacgcttc
     1681 accggtcttc tcctgcgcca acgcattcga gcggtagcgg gcgccgatcg ctttgaggaa
     1741 tgcgccggaa taggcgccgc ccaagtgatt tccctggccg ccgccaccgt aatagaagac
     1801 cttgtcgccg ccgtaggtgt cgcggatgcg tttgaagccc tcggcgatct cggtgatcgc
     1861 ggtgtcccag tcgatttcct catagctgcc gtcgggccgt cggcgcatcg gagacgtcag
     1921 cctgctgcgg ttgttctgat agtgatcgag tctcagcgcc ttgttgcagg tgtaaccctg
     1981 cgacgcggga tgttccttgt caccgcggat cttgtgaagt gtgcggccct cgagctgcac
     2041 cacgatgcca cagttgcact cgcacaggat gcaggcggtc gactgccact cttcactcat
     2101 caggatttcc tttcggccgt ctcggcttcc agcagggtgc ggagctgacg ctgcacgaga
     2161 tcaagcggtg tcacgtcgcg ggatgcgcgg gccacgatga tcgcgccctc gatcgaagtc
     2221 gtcaccaaca tcgccagttc ctccgcgcgc ccggcactca cgccctcgcc gatgaggagt
     2281 tgaccgatga gatcggtcca catcgcaaac gcggccgcgg cacgctcgat gaccttcgtc
     2341 gcgcgctcgg gattgtctgg atcgcctgcc tcgacggtca ccgcgacgac tggacagccg
     2401 gcccggaagt cgcttcggat caattgcctg cggtacaact ccgtcaggtc gtccaacagt
     2461 tcgacggcgc tttcggcagc cgagatcttg tccgcgatgt aggcgccggc gtagtcggtc
     2521 gcctcttcga gcagttgcat ccggccgccg gggaagtagt gatatgccga tccccgcggc
     2581 gcgccactgt gcttcagcac gtctgagatc gcagttgcct gggcaccgcg ttcgcgaatc
     2641 aacagtgcgg ccgatacgac catccggtcc cgcgggttgc tgaccacgct atatatgatg
     2701 gcatacataa cggacggctg gctagcgtcc gcgaacgatc actcacgtac ggttcgacct
     2761 gcgaatctgt acgacagcga gcgctcggcc gtgatccgca gccgggacgt caggttttcg
     2821 agggctgctt ttcgtctgcg caatcctcgc cgacgtcctg tgtgtcccga cgacgttcac
     2881 gcagcacgaa ggcggccagc gccagcgcca gaacgacgcc ggcgagcacg ccgatttcga
     2941 cgaccgcgcg ggcaaaggcg ggccccttgt ccagcagcgt cgcggcctcg acggacagca
     3001 cgacgatctc cttgatgccg gccaggatgc cgacgatcag gaacggttct gccacgatct
     3061 cg
//
LOCUS       DASYLX010000110         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1541314,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000110 DASYLX010000000
VERSION     DASYLX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541314"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1244
                     /locus_tag="VGP27_02625"
     CDS             219..1244
                     /locus_tag="VGP27_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV7850244.1"
                     /translation="MRATMMYAPGDVRVEERPEPTIENPTDAVISLSASCICGSDLWP
                     YRGADPVDGPITMGHEYVGIVEEVGAAVTMIRPGQFVVGSFFASDNTCEICRAGYQTS
                     CVNREILGTAGGAQAQRMRVPLADGTLVATPEVPAGDLVPDFLAASDVLGTGWFGAVA
                     AEAGPGKTVVVVGDGAVGLCAVLAASQLGAERIVAMSRHETRQRLAREFGATDIIAER
                     GDAGVERIKELTNGLGAHSVIEAVGTQESMLQAIQSTRSGGHVGYVGVTHDVILRGEE
                     LFMSHVHLHGGPAPVRRFLPDLMDRIWNRTINPGKVFDLELPLDDAADGYRAMDTRQA
                     TKVLLRP"
     gene            1276..2043
                     /locus_tag="VGP27_02630"
     CDS             1276..2043
                     /locus_tag="VGP27_02630"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7850245.1"
                     /translation="MNPSYDFTNQVALVTGAGSGMGLATARAFAENGAAVVLADINPA
                     TVKDASAELASAGHRALGVECDVSDERSVAEAVQAAVDTYGRLNMAFNNAGIQIPPCD
                     AADEPAEVFDRVNAINLRGVWACMKHELAAMRGQGSGAIVNCSSLGGLVGIPGRAAYH
                     ASKHGVLGLTSSAALEYASRGIRINAVCPGTIATPMVTDMIDKGELDRDAAIADQPIN
                     RLGEPEEIAAAVLWLCSPGASFVVGVALPVDGGYTAR"
     gene            2076..2471
                     /locus_tag="VGP27_02635"
     CDS             2076..2471
                     /locus_tag="VGP27_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7850246.1"
                     /translation="MEISQDASRPAGRGPAETFTGEVSVKPLFDVNDARTFSSAEVSF
                     TPCARTAWHTHPGGQTLIVTAGNGWVQEWGEEKQRIAEGDVIWTPPGVKHWHGATDDT
                     AVTHIAIQAFVGGRPVDWLEHVADDQYFG"
     gene            2635..3015
                     /locus_tag="VGP27_02640"
     CDS             2635..3015
                     /locus_tag="VGP27_02640"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEV7850247.1"
                     /translation="MPQSPTSPGTPIHIVIGATVITAELWDNAPARAFVEKLPLTLDF
                     SDLNAVEKIARLDVALPMTGMPDGDDPAPRDIGWYAPSGDVVLYYGDVGYWPGIARIG
                     HISGDIVSIADQQGDFTATIELAT"
     gene            3199..>4179
                     /locus_tag="VGP27_02645"
     CDS             3199..>4179
                     /locus_tag="VGP27_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850248.1"
                     /translation="MNTEHTRRTLLARIGAGAALVGLLAAAGCAPSSSETVATTTSAL
                     SGPTPNLPAGDMSNGAANFYTSDRVNVQPVSFKNQYGMNVTGNLFVPNDIDRNTTNPA
                     IVVGHPMGAVKEQSSNLYATKMAERGFVTMSLDLSFWGASDGQPRNAVSPDIYAEDFS
                     AAVDYLRTQSFVDKERVGALGICGSGSFVVSAAKIDPRIKSVATVSMYDMGAANRDGL
                     RNGVTLEQRRQVLPQAADQRDAEFRGGEAKYTGGTPEELTDQSSAIDREFYDFYRTSR
                     GNSPDTTTHPTMTSNVKFMNFYPFNDIETISPRPMLFIAGQNAHSLEFSED"
BASE COUNT          786 a         1453 c         1297 g          643 t
ORIGIN      
        1 cccgcctgct gcggagtgat cttggcccgg cgcgagagga ggaactcgcg gacctcggtg
       61 cggttgtcca cctctacgac gctacgtctc gcgctcccgg cgtgggatgc actaccggta
      121 cacgttcgga caggcagtgc ccgaatacgc cggaaggggg ttacctggac ggtgagcccg
      181 cggcgaactg cccggcaccc ttgcggagga ggacgaatat gcgcgcgacg atgatgtacg
      241 cccctggtga cgtgcgggtc gaggagcggc ccgaacccac catcgaaaac ccgaccgacg
      301 cggtcatcag cttgtcggca tcgtgcatct gcggatcgga cttgtggccg tatcgcggcg
      361 cggatcccgt cgacgggccg atcacgatgg ggcacgaata cgtcggcatc gtcgaagagg
      421 tcggcgcagc cgtcaccatg atccgccccg gccagttcgt ggtgggctcg ttcttcgcat
      481 ccgacaacac ctgcgagatc tgccgcgccg gttaccaaac gtcgtgcgtc aaccgcgaga
      541 tcctcggcac cgcaggcggt gcgcaggccc agcggatgcg ggtcccgctg gccgacggca
      601 ctctggtcgc cacccctgag gttcccgccg gcgatctcgt cccggacttc ttggcggcct
      661 ccgacgtgct gggtacgggc tggttcggcg ccgtcgccgc cgaagccggg ccaggtaaga
      721 ccgtggtagt ggtcggtgac ggcgcggtcg ggctgtgcgc ggtactggcc gccagtcagc
      781 tcggcgccga gcggatcgtg gcgatgagcc gccacgaaac ccggcagcga ctggcccgcg
      841 aattcggagc caccgacatc atcgccgagc gcggcgatgc cggggtagaa cgcatcaaag
      901 agctcaccaa cggcctgggg gcacactcgg tcatcgaagc ggtcggtacc caggaatcga
      961 tgctgcaggc catccaatcc actcggtccg gcggtcatgt cggctacgtc ggtgtcaccc
     1021 acgacgtcat cctccgtggc gaggagctgt tcatgtccca tgtccacctg cacggcgggc
     1081 ccgccccggt acgccgcttc ctgccggatc tgatggaccg catctggaac cgaacgatca
     1141 accccggcaa ggtgttcgac ctcgaacttc ccctcgacga cgccgccgac ggataccgcg
     1201 ccatggacac ccgccaagcc accaaagtgc tgctgcggcc gtgacgcgac aaccaccacc
     1261 tccgagaagg gccaaatgaa cccgtcctac gacttcacca atcaggtcgc cctggtgacc
     1321 ggcgccggat ccggtatggg attggccacc gcgcgagcat tcgccgaaaa cggcgccgcg
     1381 gtggtcctag ccgacatcaa cccggcgacg gtcaaggacg ccagcgcgga acttgccagc
     1441 gccggacacc gtgccctcgg cgtcgaatgc gacgtctccg acgaacgcag tgtggccgag
     1501 gcggtacagg ccgccgtcga cacttacggc cggttaaaca tggcgttcaa caacgccggc
     1561 atccagatcc cgccgtgtga tgccgctgat gaacccgccg aggtgttcga ccgggtgaac
     1621 gcgatcaatc tgcgcggggt gtgggcctgc atgaaacacg aactcgccgc gatgcgcggc
     1681 cagggcagcg gcgcgatcgt caactgctcc tctctgggtg ggctggtcgg cattcccggc
     1741 cgcgcggcgt atcacgcgtc caagcacggt gtgctcggcc tgacgagcag cgccgcactc
     1801 gagtacgcat ctcgcggcat ccgcatcaac gcggtatgtc cgggcacgat agccaccccg
     1861 atggtcaccg acatgatcga caagggtgaa ctcgaccgcg acgcggccat cgccgatcaa
     1921 cccatcaacc gcctcggtga gcccgaggag atcgccgcgg ccgtgctgtg gctgtgcagt
     1981 cccggcgcca gcttcgtcgt gggtgtcgcc ctgcccgtcg acggcggcta caccgcccgc
     2041 tgatccgaca tcgagcaatg agagaaggaa caggaatgga gatttcccag gacgcgtcgc
     2101 gcccggccgg tcgcgggccc gccgaaacgt tcaccggcga ggtcagcgtc aagccactat
     2161 tcgacgtcaa cgacgcccgc acgttcagtt cggccgaggt gtccttcacc ccgtgtgcgc
     2221 gcactgcctg gcacacccac cccggcggac aaaccctcat cgtcaccgcc ggcaacggtt
     2281 gggtccagga gtggggcgag gagaagcagc gcatcgccga aggcgacgtc atctggactc
     2341 cacccggggt caagcactgg cacggcgcga ccgacgacac cgcggtgacc cacatcgcca
     2401 ttcaggcctt cgtcggcggg cgccccgtgg actggctcga gcacgtcgcc gacgatcagt
     2461 acttcggcta gaagtcgacg tcgggtgcca gcagtggcca ggcccgtcgc agcggtggcg
     2521 ggggcagcga tgctctccgt actcgtcgct tgcagcacca cgacatcgct tccggataca
     2581 tcgaaaagca cggctaggca gtcgatcccg tcaaccagta gtcgagaagg gtcgatgccg
     2641 cagtcaccca cctcaccggg cacccccatt catatcgtga tcggcgccac cgtgatcacc
     2701 gccgaactgt gggacaacgc tcccgcccga gcgttcgtcg aaaagctgcc cctgacgctg
     2761 gacttcagtg atctcaatgc cgtggagaag atcgcgcgac tcgatgtcgc gctgcccatg
     2821 accgggatgc ccgacggcga tgaccccgcg ccgcgtgaca ttggctggta cgcccccagc
     2881 ggcgacgtcg tgctgtacta cggggacgtc ggctattggc ccggcatagc ccgcatcggc
     2941 cacatcagcg gcgacatcgt ttccatcgcc gaccaacagg gcgacttcac ggccaccatc
     3001 gaactggcga cctgagtcgg ccgatccccg gttgcacggg tgtaccgcca gtacacctac
     3061 ccataggacc ttcccggctc gacttctgag gtgtttcctg aatatagccg gtgcggtaag
     3121 gacgccacac ccagtcgaag tcaaaggcgc accgccacag tgtgattggc tgcgcccccc
     3181 aggataagga gcaattcgat gaacaccgag cacactcgcc gcacgttgtt ggcccggatc
     3241 ggggcgggcg cggccttggt cggcctgttg gccgccgccg gttgcgcacc gtcgagctcc
     3301 gaaaccgtcg caaccactac gtcagccctg tcgggcccca cgccgaacct gcccgcagga
     3361 gacatgtcga acggggcggc caacttctac accagcgatc gagtgaatgt gcaacccgtg
     3421 tcattcaaga accagtacgg catgaacgtc accgggaacc tgtttgtgcc caacgacatc
     3481 gaccgcaata cgaccaaccc cgcgatcgtg gtcggccacc cgatgggggc ggtcaaggaa
     3541 caaagctcca acctctacgc gaccaagatg gccgagcgtg gcttcgtcac gatgtcgctg
     3601 gacttgtcat tctggggtgc cagcgacggc caaccgcgca acgcggtctc acccgacatc
     3661 tacgccgagg acttcagtgc tgccgtggac tacctgcgca cccagtcatt cgtggacaag
     3721 gaacgcgtcg gcgcgcttgg aatctgcggc agcggcagtt tcgtggtcag cgcagcgaag
     3781 atcgacccgc gcatcaagtc ggtggccacc gtcagcatgt acgacatggg tgcagccaat
     3841 cgcgacggcc tccgcaacgg agtcaccctc gaacaacgtc gccaagtcct gccgcaggca
     3901 gccgaccagc gcgacgccga attccgtggc ggggaagcca aatacaccgg cggcactccc
     3961 gaggaactca ccgaccaatc cagcgccatc gaccgggagt tctacgactt ctaccgcacc
     4021 tcgcgtggta acagccccga caccaccacg cacccgacga tgaccagcaa cgtcaagttc
     4081 atgaacttct accccttcaa cgacatcgag acgatctcgc cacgacccat gctgttcatc
     4141 gccggccaga acgcccactc cctcgagttc agcgaggac
//
LOCUS       DASYLX010000111         6087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_663454,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000111 DASYLX010000000
VERSION     DASYLX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663454"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..937)
                     /locus_tag="VGP27_02650"
     CDS             complement(83..937)
                     /locus_tag="VGP27_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850249.1"
                     /translation="MAQSGFEGKRCFLTGAASGIGRATALKLAAQGAELYLTDRDADG
                     LTTTVADALALGAKVPEHRALDISDYDAVTRFAADIHANHPAMDVVMNIAGVSAWGTV
                     DRLTHDHWKSMIDINLMGPIHVIEAFVPPMVKARRGGHLVNVSSAAGLVALPWHAAYS
                     ASKYGLRGLSEVLRFDLARDRIGVSVVVPGAVKTPLVQTVQIAGVDREHPDVQKWTNR
                     FAGHAVSPEHVADRILRGVAKNRFLIYTSPDIRALYMFKRMAWWPYSVAMRQANVVFS
                     RALRPGLK"
     gene            complement(956..1591)
                     /locus_tag="VGP27_02655"
     CDS             complement(956..1591)
                     /locus_tag="VGP27_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850250.1"
                     /translation="MTAESAPQEIRRSRGDRQRDAIVAAVRELLHERSFADLSVSTIS
                     ERAGVARSGFYFYFDSKYAVLAVILADASELLDSLTHHFASREPGETPAAFAKRMVGS
                     AAAVYANDDPVLKACAVARNTDAQIREMMDDFYDGIIDKLIALLEQDSEARPISDDLA
                     ALVRTLAATTTMTLTQDSAFVGRGTDPRRAIDALERLWVSSIWGGAGLGNN"
     gene            1711..3195
                     /locus_tag="VGP27_02660"
     CDS             1711..3195
                     /locus_tag="VGP27_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850251.1"
                     /translation="MPTISTKEYVLDQARRRIATSPILTVPGMGLLEKRLLARDWPQH
                     EMAEPPVDSDLKPVMGDAGLPIIGHMVEMFRGGPDFFLHLYRKHGPVFLMDSPFIPYV
                     TALGPDAAQVIYSNRNKDYSQQGWTPMIGAFFNRGLMLLDFEEHMFHRRIMQEAFVRT
                     RLTGYTEQVDQVVSQVIANDWVANDARFLLYPAMKELTLDIASMVFMGHEPGTDKELV
                     TKVNRAFTVTTRAGNAIIRKGVPPFTWWRGLKAREYLENYFEQRVRERRGRDGNDLLT
                     VLCQSEDEEGNKFSDEDIVSHMIFLMMAAHDTSTSTATTMSYHLAANPQWQGRCRDES
                     DRLGDGPLDIEALEKLESLDLVMNESIRLVSPVQWAMRQTVRDTELLGHYLPKGTNVI
                     AYPGMNHRLEEIYPEPYKFDPDRFAEPRNEHKKHRYGFTPFGGGAHKCIGMVFGQLEI
                     KTIMHRLLRRYRLELPRPGYEPRYDYGGMPVPMDGMPIVLRPLR"
     gene            complement(3209..3685)
                     /locus_tag="VGP27_02665"
     CDS             complement(3209..3685)
                     /locus_tag="VGP27_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEV7850252.1"
                     /translation="MTFPSATIAFKPFAAHFDHPLPRGLRSHGEAMTWESFCAVYGGA
                     SGPLRLGSWVCGDAGRQVPHARTYQATLAVGDRVSTSSAAACGPVAALTAMLYERGIA
                     VELLGFHQISTGEQTATFINAGDGYHSEWAMGWSQDPTQSALRAVIACANRLLSAP"
     gene            3978..4607
                     /locus_tag="VGP27_02670"
     CDS             3978..4607
                     /locus_tag="VGP27_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HEV7850253.1"
                     /translation="MNGDTAIRPRNTVGSFRYFRAEDRWEWSDAVAQMHGYESAAVEP
                     TTALVLSHKHPDDAAKVTLLIEAMAGQGQPFSSRHRIIDASGRTRSILVVGEQLRDGD
                     ETVIGSQGFYVDLSELGPDQTIEAAIADFTAHRSQIEQAKGVLMAIYGISPECAFDIM
                     VWRSQETNTKLRTLVVQIIEDFTTQLNAPVNVRERADHLLLTAHTRVAR"
     gene            complement(4604..5416)
                     /locus_tag="VGP27_02675"
     CDS             complement(4604..5416)
                     /locus_tag="VGP27_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEV7850254.1"
                     /translation="MTAPDRLPHRLRDCVDQTIAAEALEGWLPTAEEVDALVALVNGD
                     VSFDDYLAANRSRYQFRPAQVSARRRLRREAPYLIPRTSLLRNNFGADSHETLAELEF
                     VATAGRIAGWHRRLAHGQVGVDDLDIPEIHRQLFADVYAWAGSYRVTDLRLGDDVFAR
                     QSSVQQMMVRIEAGARTLVADDAENRDDALARQLARFYAEYNYVHPFREGNGRTGTLV
                     LHIVSTLRGRRLDLGTISRDEWYSASRDSMPLRRDGMADPRSFVPLLLRALG"
     gene            5452..>6087
                     /locus_tag="VGP27_02680"
     CDS             5452..>6087
                     /locus_tag="VGP27_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7850255.1"
                     /translation="MTWWDAAFLAAAGVVGGLTGSVAGLASIATYPALLLVGLPPVTA
                     NVTNTVALVFNGIGSVLGSRPELQGQGPWLKRIIPVAAIGGLIGALLLLSTPAEGFEK
                     IVPILLGAASLTILLPRGGNRRSRITSRRRQTLTVLFESGVIFMICIYAGYFGAAGGV
                     VLLALLLRAGHATLAHANASKNVIAGVSNTVAALVFAFTAPVQWLAAIALGA"
BASE COUNT         1077 a         1992 c         1975 g         1043 t
ORIGIN      
        1 tgtcgtcggc ctgaccgatc agaccgacct gttcttcacg atgatcgacg ccctgggcat
       61 caagcgctga cggcggtcgc aactatttca ggcccggccg cagtgcgcgg ctgaacacca
      121 cgttggcctg ccgcatcgcc acgctgtagg gccaccacgc catgcgcttg aacatgtaga
      181 gcgcgcggat gtccggtgag gtgtagatca ggaaccggtt cttggcgacg ccgcgcagga
      241 tgcggtcggc gacgtgttcg ggtgacaccg cgtgcccggc gaaacggttt gtccacttct
      301 gcacatcggg atgctcgcgg tccacgcccg cgatctgaac cgtctgcacc agaggggttt
      361 tcaccgctcc gggtaccacg acggacacgc cgatgcggtc gcgcgccagg tcgaagcgca
      421 gcacctcgga aagaccccgc agaccgtact tgctggcgct gtaggcggcg tgccacggca
      481 gcgcgaccag cccggccgct gaggacacgt tgacgagatg gccgccgcgg cgcgccttga
      541 ccatcggtgg cacgaacgcc tcgatgacgt ggatcggccc catcaggttg atgtcgatca
      601 tcgacttcca gtggtcatgc gtgagccggt cgaccgtccc ccacgccgag acgcccgcga
      661 tgttcatcac cacatccatg gcgggatggt tcgcatggat gtcggcggcg aagcgggtga
      721 cggcgtcgta gtcggagatg tcgagcgcgc ggtgctcggg cactttcgcg ccaagcgcga
      781 gggcgtcggc gacagtcgtc gtcaagccgt ctgcgtcgcg gtcggtcagg tagagctcgg
      841 cgccctgggc ggcaagcttc aatgcggtcg cgcgaccgat accgcttgcg gcccccgtca
      901 gaaagcaacg cttgccctca aagccgctct gcgccatggc ggtgacctta ctgacctagt
      961 tgttgccgag cccggcgcca ccccagatcg acgacaccca cagccgttcc agcgcgtcaa
     1021 tggcgcgcct gggatcggtg ccgcggccga cgaacgcact gtcctgcgtc aaggtcatcg
     1081 tcgtggtcgc cgccagcgtg cgcacaaggg cggccaggtc atcggagatc ggtcgcgcct
     1141 cggagtcctg ctccagcaag gcgatgagct tgtcgatgat gccgtcgtag aagtcgtcca
     1201 tcatctcgcg gatctgcgcg tcggtattgc gggccaccgc gcaggccttc aacacgggat
     1261 cgtcgttggc atagaccgcc gccgcgctgc cgaccatccg cttcgcgaac gccgcgggcg
     1321 tctcccccgg ctcgcgagag gcgaagtggt gggtcaggct gtcgagcagc tcgctggcgt
     1381 ccgccaggat gaccgcgagc accgcgtatt tcgaatcgaa gtagaagtag aagccggatc
     1441 gtgcgacacc ggcacgctcg ctgatggtgc tgaccgacag gtcggcgaag gaccgctcgt
     1501 gaagcagttc gcgaaccgcg gcgacgatcg cgtcacgttg ccgatcgccg cggctgcgac
     1561 ggatctcctg cggggcggat tcagcggtca tgtccatcaa ctttcgcatc acgactcaca
     1621 gcggcaaaac ttgacatgcg tcaagtctgc cattcagtat gaggatagga gtgacaacgg
     1681 ccacagtttt tctgcttcag gagcatttga atgccgacga tcagtaccaa ggaatacgtc
     1741 ctcgaccagg ccaggcggcg catcgcaacg tcgccgatcc tcaccgtccc gggtatgggg
     1801 ctgctcgaga agcggctact ggcccgcgac tggcctcagc acgagatggc cgagcccccc
     1861 gtggacagcg atctcaagcc ggtgatgggc gatgccgggc tgccgatcat cggccacatg
     1921 gtcgagatgt ttcgcggcgg tcccgatttc tttctccatc tgtaccgcaa gcacggcccg
     1981 gtattcctga tggattcccc gttcatcccc tatgtgacgg cgctgggtcc cgatgcggca
     2041 caggtcattt actccaaccg caacaaggac tactcacagc agggctggac cccgatgatc
     2101 ggcgccttct tcaaccgcgg cctgatgctc ctcgacttcg aggaacacat gttccaccgg
     2161 cggatcatgc aggaggcgtt cgtccgcacc cggctgaccg gctacaccga gcaggtcgac
     2221 caggtggtct cgcaagtcat cgccaacgac tgggtcgcca acgatgcgcg cttcctgctg
     2281 tatccggcga tgaaggaact gaccctcgac atcgcctcga tggtgttcat gggtcacgag
     2341 cccggcaccg acaaggagct ggtcaccaag gtgaaccgcg ccttcaccgt cacgacgcgc
     2401 gcgggcaacg ccatcatccg caaaggcgtg ccgccgttca cgtggtggcg cgggctgaag
     2461 gcccgtgagt acctggagaa ctacttcgag cagcgggtga gggagcgccg cggcagggac
     2521 ggcaatgatt tgctgaccgt gctgtgccag tcggaggacg aggagggcaa caagttctcc
     2581 gacgaggaca tcgtcagcca catgatcttc ctgatgatgg ccgcccacga cacgtcgacg
     2641 tccaccgcga cgacgatgtc atatcacctg gcagccaacc cgcagtggca gggacgctgc
     2701 cgcgacgagt ccgaccggct cggtgacggg ccgctcgaca tcgaggcgct ggagaagctc
     2761 gagtcgctcg acctagtgat gaacgagtcg atccggctgg tctcacccgt gcagtgggcg
     2821 atgcgccaaa ccgtgcgcga caccgaactg ctcggccact acctgcccaa gggcaccaac
     2881 gtgatcgcgt atccgggaat gaaccaccgg ctcgaggaga tctaccccga gccctacaag
     2941 ttcgacccgg accgcttcgc cgagccgcgc aacgagcaca agaaacatcg ctacgggttc
     3001 accccgttcg gtggcggtgc gcacaagtgc atcgggatgg tgttcggcca gctcgagatc
     3061 aagacgatca tgcacaggct gctgcgtcgg taccgcctcg agctgccccg cccgggatat
     3121 gagccgcgct atgactacgg cggcatgccg gtgccgatgg acggcatgcc gatcgtgttg
     3181 cggcccctga ggtgacggcg tcggaccgct agggcgcact caacaaccgg ttggcgcagg
     3241 cgatgacggc gcgcaacgcc gattgcgtcg ggtcctgcga ccaccccatc gcccactcgg
     3301 agtggtatcc atcacccgcg ttgatgaatg ttgcggtctg ctcgccggtt gatatctggt
     3361 gaaaacccag cagctccact gcgattcctc gctcgtagag catggcggtc aatgcggcga
     3421 ccggaccgca agcggcggct gacgacgtac tgacgcgatc gccgaccgcc aacgtcgcct
     3481 gatatgtgcg cgcgtgcggt acctgacggc ccgcgtcgcc acacacccag ctacccagtc
     3541 gcagtggacc agaggcgccg ccgtacaccg cgcagaacga ctcccacgtc atcgcttcgc
     3601 cgtggctgcg cagcccacgg ggcaacgggt ggtcgaaatg cgcggcgaac ggcttgaatg
     3661 caattgtcgc cgaagggaat gtcatatgcc ggtcttctct cgggggaggg agtgaagtga
     3721 ccgacgacgt agcaacgacc cacagcgagg ggtcggtcag gatcagaccc cgctggggat
     3781 tgctactacg agtcgcctcg gcacgccacg aagactagac ctgttccgcg aacagccgca
     3841 acttcgtttt ttggactgtg gtggcgcaat ctgttcgctg cgcgcgctga ccgcgaatcg
     3901 ccgaggtggg atatttcgtc tacttggccg caggtaggca gggcgaggtg agtgcgttga
     3961 gaaattgcag cgctgacatg aacggggata cggcgattcg accgaggaac acggttggct
     4021 cgttccgcta cttccgtgcc gaggaccggt gggaatggtc cgacgccgtc gctcagatgc
     4081 acggatatga gtctgcggcc gttgagccga cgacggcgct ggtgctgtcg cacaagcatc
     4141 cagacgatgc tgcgaaagta acgctgctga tcgaggcgat ggcgggccag ggtcagccgt
     4201 tcagcagcag gcatcgcatc atcgacgcat ccggccgcac ccgcagcatc ctggtagtgg
     4261 gcgaacaact gcgcgacggt gacgagacgg tcatcggcag ccagggcttt tatgtggacc
     4321 tcagcgaact cgggcctgac cagaccatcg aggccgcgat cgcggatttc acggcgcacc
     4381 ggtcgcagat cgagcaggcc aagggcgtcc tgatggccat ctacggaatc tcccccgagt
     4441 gtgcgttcga catcatggtc tggcgttcgc aggagaccaa caccaagctg cgcacgctgg
     4501 tcgtgcagat catcgaggac ttcaccaccc aactgaacgc gcccgtcaat gtccgcgagc
     4561 gggccgacca cttgctgctc accgcccaca cgcgggtcgc ccgctagccg agcgctcgca
     4621 ggagtaacgg cacgaacgac cggggatcgg ccatgccgtc gcggcgaagc ggcatgctgt
     4681 cgcgcgatgc ggaataccac tcgtcgcgcg agatcgtgcc gagatcgagc cggcgacctc
     4741 gcagcgtcga cacgatgtgc agcaccaatg tcccggtgcg cccgttgcct tcgcggaagg
     4801 gatgcacgta gttgtactcg gcgtagaacc gggcgagttg acgggccaac gcgtcgtcgc
     4861 ggttctctgc gtcgtctgcc accagggtcc gcgcgcccgc ctcgatgcgg accatcatct
     4921 gctgcaccga cgattgccgg gcgaacacgt cgtcccccag tcgcaggtcg gtgacgcggt
     4981 aactgcctgc ccacgcgtag acgtcggcga acagctgccg gtggatctcg ggtatgtcga
     5041 gatcgtcgac accgacctgg ccgtgagcga gcctgcggtg ccagcccgcg atccgccccg
     5101 cggtggcgac gaattccagt tcggccagcg tttcatggct atcggccccg aagttgttgc
     5161 gcagcagcga ggtccgcggg atcagatagg gcgcctcgcg acgaagccgt cgccgggccg
     5221 atacctgggc gggccgaaac tggtatcgcg aacgattcgc ggccaagtag tcgtcgaagg
     5281 acacgtcgcc gttgaccaac gccaccaacg cgtcgacctc ctcggcggtc ggcagccaac
     5341 cctccagcgc ttcggccgcg atcgtctggt cgacgcagtc gcgcagccga tgaggcagcc
     5401 ggtcgggggc ggtcacggcg ttgattctgc gagcaggcac gataatggtc aatgacttgg
     5461 tgggacgccg ccttcctcgc cgccgccggt gtcgtgggag ggctgaccgg cagcgttgcc
     5521 ggactggcgt cgatcgccac ctacccggcc ctgctgctgg tcggactgcc gccggtcacc
     5581 gccaacgtga ccaacaccgt ggcgttggtg ttcaacggga tcggctcggt gctgggttca
     5641 cggcccgaac ttcagggtca gggtccctgg ctgaagcgga tcatcccggt cgcggccatc
     5701 ggcgggctca tcggcgccct actgctcctg tcgacacccg ccgaaggctt cgagaagatc
     5761 gtgccgatct tgcttggggc ggcgtcgctc acgatcctcc ttccgcgagg aggcaatcgg
     5821 cggtcacgca tcaccagtcg ccggcgccag acccttacag tcctgtttga atccggcgtg
     5881 atcttcatga tctgtatcta cgcaggctat ttcggagccg cgggcggtgt ggttttgctc
     5941 gctctcctgt tgcgcgccgg ccatgcgacg ctcgcccacg ccaacgcctc caagaacgtc
     6001 atcgccggtg tctccaacac cgttgcggcg cttgtcttcg cgttcaccgc cccggtgcag
     6061 tggctcgccg ccatcgcgct gggcgcc
//
LOCUS       DASYLX010000112         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1321475,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000112 DASYLX010000000
VERSION     DASYLX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321475"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2059)
                     /locus_tag="VGP27_02685"
     CDS             complement(<1..>2059)
                     /locus_tag="VGP27_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEV7850256.1"
                     /translation="DRIAQTESLFALSNGHIGLRGNLEEGEPHGLPGTYLNGYYETRP
                     LPYAEAGFSYPEDGQSIVNATNGKILRLLVDDEPFDVRYGELHEHERVLDMRAGILTR
                     NARWTSPAGKQVAVRTTRLVSLDQRGVAAMEYIVEAVDNFVNIIVQSELVANEDQPDR
                     SKDPRTAALLGNALEAVRHESTDHGAVLIHKTRQSGLMMAAAMEHDIEVPGRVETSSE
                     AREDLARTTVICGLRPGQQLRIVKYLGYGWSSLRSRPALRDQVAGAIAGARYAGWQGL
                     IDAQREYLDGFWDSADVEVEGDPACQQAVRFGLFHVLQASARAERRAIPGKGLTGTGY
                     DGHAFWDTEGYVLPVLTYTAPQAAADALRWRMSTIDLARRRAEELDLAGAAFPWRTIH
                     GEECSAYWPAGTAAWHVNADIAMAFERYRVVTGDDSLEADCGVVVLVETARLWLSLGH
                     HDRHGVWHLDGVTGPDEYTALVRDNVFTNLMAMHNLQTAADACERNPEMASTLGVTTE
                     EAAAWRDAAGAAHIPYDEELGVHEQCAGFTTLREWNFTENTAYPLLLHEPYVRLYPSQ
                     VIKQADLVLAMHWRSHAFGPEERARNLDYYERRTTRDSSLSACTQAVMCAAVGHLELA
                     YDYTYEAALIDLRDLHDNTEDGLHMASLAGAWTALVAGFGGLRDDQGVLALDPQLPDG
                     IER"
BASE COUNT          328 a          706 c          671 g          354 t
ORIGIN      
        1 aaccgttcga tgccgtcggg cagttgcggg tcgagcgcga gaacgccttg gtcgtcgcgc
       61 agccctccga atccggccac caacgccgtc cacgcgcccg ccagcgacgc catgtgcagg
      121 ccgtcctcgg tgttgtcgtg cagatcgcgc aggtcgatca gcgcggcctc gtaggtgtag
      181 tcgtaggcca gctcgaggtg tccgacggcg gcgcacatca ccgcctgcgt gcacgccgac
      241 agggacgagt cgcgggtggt gcgccgctcg tagtagtcga ggttgcgcgc cctctcctcg
      301 ggtccgaagg cgtgactacg ccagtgcatc gccaacacca ggtcggcctg cttgatgacc
      361 tgcgaagggt acaaccggac atagggctca tgaagcaaca gcgggtaggc ggtgttttcg
      421 gtgaaattcc actcgcgcag cgtggtgaac ccggcgcact gctcgtgcac gccgagctct
      481 tcgtcgtagg gaatgtgggc ggcccctgcg gcgtcccgcc acgcggccgc ttcctcagtg
      541 gtcacgccga gtgtgctggc catctcggga ttccgctcgc aggcgtcggc ggcggtctgc
      601 aggttatgca tcgccatcag gttggtgaac acgttgtccc gcacgagcgc ggtgtactcg
      661 tcggggcccg tgacaccgtc gaggtgccac acgccatgac ggtcatgatg tcccagcgag
      721 agccacagcc gcgcagtctc gacgaggacc acgacgccgc agtccgcctc cagcgaatca
      781 tcgccggtga cgacacgata ccgctcgaag gccatcgcga tgtccgcgtt gacgtgccac
      841 gcggcggtgc cggcgggcca atacgccgag cactcctcgc cgtggatggt gcgccacgga
      901 aacgcggcgc cggcgaggtc tagctcctcg gcgcgcctgc gggccagatc gatcgtcgac
      961 atccgccacc gcagcgcatc ggcggcggct tgtggcgcgg tgtaggtcag tacgggcagg
     1021 acatagccct cggtgtccca aaaggcgtgg ccgtcgtaac ccgttccggt caggcccttg
     1081 cccggtatcg ctcgccgttc ggcacgtgcg ctggcctgta acacgtgaaa cagcccgaac
     1141 cgcaccgcct gctggcacgc cgggtcgccc tccacctcga catcggcgga atcccaaaac
     1201 ccgtcgaggt actcacgctg cgcatcgatc aggccctgcc agcccgcgta tcgtgctccg
     1261 gcgatcgctc cggcgacctg atcacgcaat gcgggacggg accgcaggct cgaccacccg
     1321 tagcccaggt acttgacgat gcgcagttgc tgaccagggc gcaggccgca gatcacggtg
     1381 gtacgggcca agtcctcgcg agcctccgaa ctggtctcga cgcgcccggg cacctcgatg
     1441 tcgtgctcca tcgcagcggc catcatcaac ccgctctgcc gggttttgtg gatcagcacc
     1501 gcaccgtggt cggtcgattc atggcgcacc gcctcgagcg cattgcccag cagcgcggcc
     1561 gtgcgcggat ctttggaccg gtccggctga tcctcgttgg cgacgagctc cgactgcacg
     1621 atgatattga cgaagttgtc gaccgcctcg acgatgtact ccatggcggc cacgccgcgc
     1681 tgatcgagcg agaccagccg cgtcgtccgc acggccacct gcttacccgc cggtgatgtc
     1741 caccgcgcgt tgcgggtgag gatgcccgcc cgcatatcca aaacccgttc gtgctcgtgt
     1801 aattcgccgt agcggacatc gaacggctcg tcgtcgacca gcagccggag gatcttgccg
     1861 ttggtggcgt tgacgatcga ctgaccgtct tcgggatagg agaatccggc ctccgcgtac
     1921 ggcagcggcc gggtctcgta gtacccgttc agatacgtgc cgggcagccc gtggggttca
     1981 ccctcctcga gatttccccg cagcccgatg tggccgttgg acaacgcgaa cagtgattcg
     2041 gtctgggcga tcctgtcca
//
LOCUS       DASYLX010000113         7340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1760363,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000113 DASYLX010000000
VERSION     DASYLX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760363"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VGP27_02690"
     CDS             <1..447
                     /locus_tag="VGP27_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850257.1"
                     /translation="TVDEASSTTYLDGTSSTSANAITTGDTVLVLGTTSGTTITARQV
                     VMQPTDGGGAAASSAAGVIPFQRGAPTTSNQVGRIPADYTQGSGTIVSGTAADSATEA
                     ALAAYPGGVVDRVVQLTSGEYEVHYIGVNWPHHIFVDQEFTVVGAY"
     gene            complement(444..1397)
                     /locus_tag="VGP27_02695"
     CDS             complement(444..1397)
                     /locus_tag="VGP27_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEV7850258.1"
                     /translation="MREPERDLVGYGANRPTTGFPDGKRLAISLVVNYEEGSERSLAS
                     GDPDQEALTEWGQYPFPRDIRNLAMETMHEFGSRVGVWRILDILKRHDVKSTFFACAV
                     AFEHAPDVARAAVEQGHEICSHGYRWEEVFRLSEDQEREHIRLAVESFERVCGKRPVG
                     WYCRYGPSTRTRRLLVEEGGFVYDSDSYNDEVPYFTEVEGRQHLVVPYTPDANDIVFW
                     LGNGMATAEQFETYLRDSFDVLYAESERYPRMMSIGLHCRVIGRPGRSPSLDRFIDYA
                     KRFDGVWFATREEIARSWLAVHGGNAHSAAGDVNAGSSPGH"
     gene            complement(1471..2664)
                     /locus_tag="VGP27_02700"
     CDS             complement(1471..2664)
                     /locus_tag="VGP27_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7850259.1"
                     /translation="MRNKTRSLVPRRRWAFTLAVVIIVALITALAVGCGGADGDKTAD
                     GSASGTCDLPYVTKQIDDHRGIAEWAYPGEPFDATKAAGRTIFTIQESSTNPFTNTIV
                     AGMKDVADRIGIELVDYPNQGQHAQWIQGIQQAISQKVDVIVLLGGAIGPIYFQEQAA
                     AAERAGIPIVTVVDRDLTQDAEPHTAARVGQPYAEAARLDADWIIKQTNCRANVLVIT
                     SNELIAGDINTKAAQDEFDKSCGEGCTTRFVNVPLAQWATRIGTTTQTEVSSNHDLNY
                     VFPLYDAMSQFVVPAIQLGGGITRVKVATFNGTPGILRMIQTGNTVEMIVGENETHLG
                     YAAMDQAMRLMTGVEPIGNGDYGIPLRIFDDSNVDETGVPPEYGVGYGQQWRDGFLKA
                     WGLSG"
     gene            complement(2690..3658)
                     /locus_tag="VGP27_02705"
     CDS             complement(2690..3658)
                     /locus_tag="VGP27_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850260.1"
                     /translation="MSVGEFVERFALVGAWIVVVVVFSVLKPDTFATSSNFAAILGSQ
                     AVLVVLTCALLIPLTAGDYDLSITGVLTLSQMMIGILNTRYGISVYVCIVLALLMGAL
                     VGLINGAFVVLLGIDSFIVTLGTGTFLGGVVLWISNSETFAGISSQLTGPVVVERLLG
                     VPLAFYYGLALVIVLWYFLEYTSLGRQLLFVGRSRSVARLSGLRVGRLRWGALVASGV
                     IAAAAGVLYAGITGAADPVSGAEFLLPAFAAAFLGATSIVPGRFNPWGSFIAVYFLVT
                     GITGLALFGIQSWLQDMFYGGALVLAVTFSQIARRREEHESGVLEN"
     gene            complement(3703..5280)
                     /locus_tag="VGP27_02710"
     CDS             complement(3703..5280)
                     /locus_tag="VGP27_02710"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV7850261.1"
                     /translation="MSEHPPVVALRHISKTFGAVRALDDVDLTIRRGEVHGLLGENGS
                     GKSTLIKVLSGFYTPDRGSELEINGRPVSLPLYPGQFRSLGMSFVHQDLGLVPSLSVV
                     DNLLVGDLISQRRWRLSWTRERRKAVETFRSFGLTIDPRAKVSDLRPTDRALLAIVRA
                     VAEIRAHTADDGSEYGLLVLDEPTVFLPASDRDQLFAIVREIAATRASILFVSHDLHE
                     ILEITDRVTVLRDGVVRGTVDTDRASDEVLVEMIIGRRIQAHSIERRKPEVGTAVSIA
                     GLTGRYARDVSLTVRAGEVVGLTGLLGSGFEEIPYCVFGARRATAGRLDVGGKTYDLR
                     AMSPSRALEAGVALVPADRQRDGTVGSLSVGDNVAVPVLHMYRSRIGLHRSRLRSDAR
                     RLLEQFDVRPPDPRLTYDSLSGGNQQKAMLAKWLQTKPSLLLLHEPTQGVDVGARQQI
                     FQLMRKAAADGTAVMCASTDYDQLAAVCDRVVVFGRGRLRRELTGDQITKERIAEQCY
                     SAAENHSGSHLDAGGDA"
     gene            complement(5277..5420)
                     /locus_tag="VGP27_02715"
     CDS             complement(5277..5420)
                     /locus_tag="VGP27_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850262.1"
                     /translation="MSSAERAAVNGESRPDLVGFQAMDDDGAGGAGVLDHLRKGPVEK
                     AAG"
     gene            5496..>7340
                     /locus_tag="VGP27_02720"
     CDS             5496..>7340
                     /locus_tag="VGP27_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7850263.1"
                     /translation="MGRTSQLVNDELAQLLDLSQDAFYIADLDGQLTFVSPALARSLG
                     YTPEELLARPFLDNVHPDDVESVGEILLGHPDGTDAQGCACRLVRADGSVCWFTLTTR
                     IRPDEGLVYGVAREVADRRGASAELTALHRVATLVAEGVQPQDLFAVVAEEVSRVVDV
                     SAVSIVRYELDGTASECANFNRGDRLFPVGVRWSIEGTNILRLVRDSAAVARIDDYAG
                     LDGELAAFVRSTGIRSTVGVPIVVAGRLWGTIVGSTTEPDPLPEDTASRLASLSELLA
                     TAIETARSREALERLVDEQAALRRVATLVASGVRPDEIFAAVSNEVGRLVGSDSATVI
                     RYDDDGEGISYVGSASKVSGAFPVGVHWKFQEGMASFDVHRTGRSARSGAHLITVDGP
                     IGDLHRRMGIVSAVASPIVVEGRLWGAMAVHGQQPLPLDTEKRLERFTQLVATAIANA
                     EGHAALTRLAEEQAALRRVATLVAQGVRPDEIFAAVSNEVGRLVGTDSATVIRFDDDG
                     EGIFYVGSASKVNAAFPVGAHWKFQEGMASAEVHRTGRSARGGARDWSKVTGPVGETH
                     HRLGIVSTVASPIVVEGRLWGAMAVQSQQPLPLDTDERLEKFTELVATA"
BASE COUNT         1221 a         2465 c         2449 g         1205 t
ORIGIN      
        1 accgttgacg aggcgtcctc tacgacctac ctcgacggga cgagctcgac gtcggcgaat
       61 gccatcacga ccggtgacac ggtcctcgta ctcgggacca cgagcggaac gaccatcacg
      121 gccaggcaag tcgtcatgca acccaccgac ggcggcggag ctgcggcttc ctcggcggca
      181 ggggtgatcc ccttccagcg cggagcaccg accacgtcga atcaggtcgg caggatcccg
      241 gcggactaca cccagggatc agggaccatc gtcagcggaa cggcggcaga cagcgcgacg
      301 gaagctgcgc tggcggccta cccggggggc gtcgtcgacc gtgttgtgca gctgaccagc
      361 ggcgagtacg aggtccacta catcggcgtc aactggccgc accacatctt cgtcgaccag
      421 gagttcacgg tcgtcggcgc ctactagtgg ccaggtgatg atcctgcatt gacgtctccc
      481 gccgccgagt gtgcgtttcc gccgtgcacc gccagccacg agcgggcgat ctcttcgcgc
      541 gtggcgaacc agacgccgtc gaaccgcttc gcataatcga tgaaccggtc gagcgagggc
      601 gagcggcccg gccgtccgat cacccgacag tgcagcccga tcgacatcat gcgtggatag
      661 cgttctgact cggcgtacag cacatcgaat gaatcgcgca ggtaggtctc gaactgctcg
      721 gccgtggcca tgccgttgcc cagccagaac acgatgtcat tcgcatcggg cgtgtagggc
      781 acgaccaggt gctgacggcc ctcgacctcc gtgaagtacg gaacctcgtc gttgtaggag
      841 tccgagtcgt agacgaaccc gccttcctcg acgagcagcc tgcgcgtccg cgtgctcggt
      901 ccgtagcggc agtaccagcc gacgggccgc ttcccgcaca cacgctcgaa cgactcgacc
      961 gcgagtcgga tgtgctcgcg ctcctggtct tcgctcaagc ggaagacttc ctcccaccgg
     1021 tagccgtggc tgcagatctc gtgcccttgc tccaccgctg cacgcgcgac gtcgggggcg
     1081 tgctcgaacg cgaccgcgca ggcgaagaac gtgctcttga cgtcgtggcg cttgaggatg
     1141 tcaaggatgc gccagacacc gactcgggag ccgaactcgt gcatggtctc catcgcaagg
     1201 ttccggatgt cgcgcgggaa cggatactgc ccccactccg tgagtgcctc ctggtcagga
     1261 tcgccggaag cgagcgaccg ctccgatccc tcctcgtaat tgacgacgag cgagatcgcc
     1321 agcctcttgc cgtcaggaaa gccggtcgtg ggccgattcg ccccgtagcc gaccaggtcg
     1381 cgttcgggtt ccctcatctt gctgccacct ccttcgcgct gggcggggag gacaccggat
     1441 gtcggggcgc cgctcctttc gctggtcttg ctagccggac agcccccatg ccttgaggaa
     1501 gccgtcgcgc cactgctggc cgtagccgac gccgtattcg ggcggaacgc ccgtctcgtc
     1561 gacgttcgag tcgtcgaaga tgcgaagcgg tatgccgtag tcgccgttcc cgatcggttc
     1621 gacgccggtc atcagacgca tcgcctggtc catcgccgcg tagccgaggt gggtctcgtt
     1681 ctcaccgacg atcatctcca ccgtgttccc ggtctggatc atgcgaagga tccccggcgt
     1741 gccgttgaag gtcgcgacct tgaccctcgt gatcccgcca ccgagctgga ttgcagggac
     1801 tacgaactgc gacattgcgt cgtacaacgg aaagacgtag ttgagatcgt ggttcgacga
     1861 gacttccgtc tgcgtcgtcg tcccgatcct cgtcgcccac tgggcgagcg ggacgttcac
     1921 gaacctggtc gtgcacccct caccgcaaga cttgtcgaac tcgtcctgtg cggcctttgt
     1981 gttgatgtcg cccgcgatca gctcgttact ggtgatgacc agcacgttcg cccggcaatt
     2041 cgtctgcttg atgatccaat ccgcgtcgag ccgcgcggcc tcggcgtagg gctggccgac
     2101 gcgtgcggcc gtgtgcggct cggcgtcctg ggtcaggtca cggtcgacga ccgtgacgat
     2161 cgggatgccc gctcgctcgg cagcggctgc ctgttcctgg aagtagatcg ggccgatcgc
     2221 gccacccagc aggacgatca cgtcgacttt ctgggagatc gcctgctgga ttccctggat
     2281 ccattgcgcg tgttggccct ggttcggata gtccacgagt tctatcccga tcctgtcggc
     2341 cacgtccttc atgccggcga cgatcgtgtt ggtgaacggg ttggtggagg actcctgaat
     2401 ggtgaagatc gtccttccgg ccgccttcgt cgcatcgaag ggttctcccg ggtacgccca
     2461 ctcggcaatc cctctgtggt cgtcgatctg cttggtcacg taggggagat cgcacgtacc
     2521 cgaggccgaa ccgtcagccg tcttgtcccc atcggctccg ccgcagccca cagcgagcgc
     2581 ggtgatcagg gcgacgatga tcaccacagc gagggtgaac gcccagcgcc ggcgcggcac
     2641 caggcttcgg gtcttgttcc tcacatcgac tcccttctcc caccaggtgc tagttctcca
     2701 gcacgccgct ctcgtgctcc tcgcggcgcc gggcaatctg cgagaacgtg acggccaaga
     2761 cgagcgcgcc gccgtagaac atgtcctgga gccacgactg gatcccgaag agcgccagcc
     2821 cggtgatccc ggtgacgagg aagtagacgg cgatgaacga tccccatgga ttgaaccggc
     2881 cggggacgat gctggtcgct ccgaggaacg ccgccgcgaa agcaggcaac aagaactccg
     2941 cgcccgacac cgggtcggcc gcgcccgtga tccctgcgta caggacgccg gcggcggccg
     3001 cgatgacgcc cgaggcgacc agtgcccccc aacggaggcg tccgacacgc agaccgctga
     3061 gccgggcaac actgcgactt cgcccgacga acagcagttg tcggcccaga gaggtgtact
     3121 cgaggaagta ccagagcacg atgacgagtg cgaggccgta gtagaaggca agagggacgc
     3181 cgaggagtcg ttcgacgacg accggcccgg tgagctggga ggagatcccg gcgaaagtct
     3241 ccgagttgct gatccacagg acgacgccgc cgaggaacgt tcccgtgccg agcgtgacga
     3301 tgaacgagtc gatgccgagg aggacgacga acgcgccgtt gatcagaccc acgagcgccc
     3361 ccatcaggag cgcgagcacg atgcagacgt atacggagat gccgtatctg gtgttcagga
     3421 tcccgatcat catctgcgag agcgtcagca cgccggtgat cgacaagtcg tagtctcccg
     3481 cggtcagcgg gatcagcagc gcgcacgtga gcacgacgag gaccgcctgc gaaccgagga
     3541 tcgcggcgaa gttggacgag gtggcgaacg tgtccggctt gagcacactg aagacgacga
     3601 ccacgacgat ccacgcgccg acgagcgcga atcgctcgac gaactcgccg acgctcatcc
     3661 ctctgcgcag ccgttgggcg aatgaagcgg tctcagcgct cgttacgcat cccctcccgc
     3721 gtcgagatga ctaccggagt ggttctcagc ggcgctgtag cactgctccg cgatccgctc
     3781 cttggtgatc tgatcacccg tcaactcgcg tctcaggcgt ccgcgaccga agacgacgac
     3841 ccggtcgcag actgccgcga gctgatcgta atcggtgctt gcgcacatca cggccgttcc
     3901 gtcggcggca gccttcctca tcagctggaa gatctgttgg cgggctccga cgtcgacgcc
     3961 ctgcgtcggc tcgtggagca acagcagcga cggcttcgtc tgcagccact tcgcgagcat
     4021 cgccttctgc tggttgccgc cgctcaagga gtcgtacgtc aatcgggggt cgggcggccg
     4081 gacgtcgaac tgttccagca gccgtcgcgc gtcgcttcgc aggcggcttc gatgcagacc
     4141 gatgcgcgac cggtacatgt gaagaacggg caccgcgacg ttgtccccga cggagagcga
     4201 gccgacggtt ccgtcgcgct ggcgatcggc cggcaccaac gcgacgccgg cctcgagcgc
     4261 acggctcggg ctcatggcgc ggaggtcgta ggtctttccg cccacgtcga gccggccggc
     4321 ggtggcgcgt ctcgcgccga acacgcagta cgggatctcc tcgaacccgc tgcccagcag
     4381 cccggtcagg cccacgacct cgcccgcacg gaccgtgagc gacacatctc gcgcatatcg
     4441 gccggtcagg cccgcgatcg ataccgccgt gccaacctcc ggcttcctgc gttcgatcga
     4501 gtgcgcctga atccgtcggc cgatgatcat ctcgacgaga acctcgtcgc tcgctcgatc
     4561 ggtgtcgacc gttccgcgga cgaccccgtc ccgcagcacg gtgacgcggt cggtgatctc
     4621 gaggatctcg tggaggtcgt gggagacgaa caggatgctg gcgcgcgtcg ctgcgatctc
     4681 ccggacgatc gcgaagagct ggtcgcggtc gcttgccggc aggaagaccg tgggctcgtc
     4741 gaggacgagc agcccgtatt cggacccgtc gtcagcggtg tgcgcacgga tctccgcgac
     4801 cgctcggacg atcgcgagca gcgcgcggtc ggtcgggcgc aggtcagaga ctttcgcgcg
     4861 gggatcgatc gtgagcccga aggatctgaa ggtctccacc gccttgcgcc tttcgcgcgt
     4921 ccacgagagt cgccaacggc gctgggagat caggtcgccg acgaggaggt tgtcgacgac
     4981 cgacagcgag ggaacgagcc cgaggtcctg atgcacgaag ctcatgccca gactgcggaa
     5041 ctgccccggg tacaacggga gcgagacggg ccggccgttg atctcgagct cgctgcctct
     5101 gtccggagtg tagaagccgg agagcacctt gatcagggtc gacttgcccg aaccgttctc
     5161 tccgagcagg ccgtgcacct cgccgcgccg aatcgtcagg tcgacgtcat ccagtgcgcg
     5221 cacagcgccg aacgtcttcg agatgtgcct gagcgcgacg accggcggat gctcgctcac
     5281 ccggcggcct tttccaccgg acccttgcgg aggtggtcga ggaccccggc gccgccggca
     5341 ccgtcgtcgt ccatggcctg gaacccgacg aggtccggtc gggattcccc gttcacagcg
     5401 gcacgctccg cgctgctcat cgtccgaggc ctgccggcca ggcgctccag accgtgccct
     5461 tccgcagggc gtacggagag gccacactgg ggagcgtggg ccgcacgagc cagctcgtca
     5521 atgacgagct ggcacagctc ctcgacctgt cgcaggatgc cttctacatc gcagatctcg
     5581 acgggcagtt gacgttcgtc agtcccgcgc tcgcgcgcag cctcgggtac acgccggaag
     5641 agctgctggc caggccgttc ttggacaatg tccaccccga cgatgtcgaa tccgtcggcg
     5701 agatcctgct cgggcacccg gacggcacgg acgcacaggg ctgcgcgtgt cgcctggtcc
     5761 gcgccgacgg ctcggtgtgc tggttcacgt tgaccacgcg catccgaccg gacgaaggtc
     5821 tcgtgtacgg cgtggcgagg gaggtcgcgg accggcgtgg agcaagcgcc gagctgaccg
     5881 cgctgcaccg cgtagcgacc ctcgtggcgg agggcgtgca gccgcaggac ctcttcgccg
     5941 tcgtcgccga ggaggtctcc cgcgtcgtcg acgtctcggc cgtgagcatc gtgcggtacg
     6001 agctcgacgg caccgcgagc gagtgcgcca acttcaaccg gggagacagg ttgttcccgg
     6061 tcggagtccg gtggtcgatc gaaggcacga acatccttcg actcgtccgg gacagcgccg
     6121 cggtcgcccg catcgacgac tacgcggggc tcgacggtga gttggcggcg ttcgtccgta
     6181 gcaccgggat ccgttcgacg gtcggagttc cgatcgtcgt cgccggtcgc ctctggggaa
     6241 cgatcgtcgg ttccaccacg gagccggatc cgttgccgga ggacaccgcg tcgcgtctgg
     6301 ccagcctcag cgagctgctc gcgaccgcga tcgagactgc caggtcgcgc gaggctctcg
     6361 aacgactcgt cgacgagcag gcagcgctcc gccgcgtcgc gacgctggtg gcgagcggcg
     6421 tccggcccga cgagatcttc gcggccgtca gcaacgaggt cgggcgcctg gtcggttcgg
     6481 attcggccac ggtcatcagg tacgacgacg acggtgaggg gatcagttac gtcggctccg
     6541 ccagcaaggt cagcggcgcc ttcccggtcg gcgtgcactg gaagttccag gaggggatgg
     6601 cttccttcga tgtgcaccgc accggacgct ctgcccgcag cggagcgcac ttgatcacgg
     6661 tcgacgggcc cattggcgac ctccaccgtc ggatgggcat cgtctccgcg gttgccagcc
     6721 cgatcgtcgt ggagggccgg ctctggggcg ccatggccgt ccatggccag cagccgctgc
     6781 cgctggacac cgagaaacgc ctcgagaggt tcacccagct cgtcgcgacc gcgatcgcga
     6841 acgccgaggg ccacgcagcc ctcaccaggt tggcggaaga gcaagcggcg ttgcgccgcg
     6901 tggcgacgct ggtggcgcag ggcgtccgac ccgacgagat cttcgcggcc gtcagcaacg
     6961 aggtcgggcg cctggtcggt acggattcgg caacggtcat cagattcgac gacgacggtg
     7021 aggggatctt ctacgtcggc tccgctagca aggtaaacgc agccttcccg gtcggcgcgc
     7081 actggaagtt ccaggagggg atggcctcgg cggaggtgca tcggaccgga cgctctgccc
     7141 gcggcggcgc gagggactgg tccaaggtca cagggcctgt tggcgaaacc caccaccgtc
     7201 tgggcatcgt ctcgacggtt gccagcccga tcgtcgtcga gggccggctc tggggcgcca
     7261 tggccgtcca gtcccagcag ccgctgccgc tggacaccga cgagcgcctc gagaagttca
     7321 ccgagctcgt cgcaaccgcg
//
LOCUS       DASYLX010000114         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_663675,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000114 DASYLX010000000
VERSION     DASYLX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663675"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGP27_02725"
     CDS             <1..249
                     /locus_tag="VGP27_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-beta-D-heptose 1-phosphate
                     adenosyltransferase"
                     /protein_id="HEV7850264.1"
                     /translation="GTNGISVRHGLSTPDSEEAAVKRAMIASANYVVVAADSSKVGRE
                     DFISFAPLSSVDALITDSEISGAYREQLTEQGIEVVVA"
     gene            246..1223
                     /locus_tag="VGP27_02730"
     CDS             246..1223
                     /locus_tag="VGP27_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV7850265.1"
                     /translation="MIVTVTPNPSLDRTVTLATPLTRGAVHRVNSVTTEPGGKGVNVA
                     RALALAGVDAVALLPASGTDPMVTALQSIGVPFRCVPITSPVRTNIAVTEMDGTTTKI
                     NEPGARLDGAAVDALTRSVVANAGHASWVVMSGSLPPGMPESWYADVVAQLAPYDCKV
                     AVDTSDRPLAALVDGFDRAAPDLIKPNAEELAGVIGFAPQALEAALSQGDPEPVVSAA
                     RQLLDRGVGAVLATLGAAGAVLVTDTGSWLATPPPILPRSTVGAGDSSLAGYLRADVG
                     GAEPAQRLQMAVAYGSAAAALPGSALPTPSEIDLDDVVVTSIPLITNPS"
     gene            1244..>2968
                     /locus_tag="VGP27_02735"
     CDS             1244..>2968
                     /locus_tag="VGP27_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HEV7850266.1"
                     /translation="MTSTASRPIIAVDLVLLDVDAGADKQAVIGRLVGRLADAGRTTD
                     PDGLIAAAMAREEQSATGLPGGIAIPHCRSPHVDTASVGFARLHPPVDFGAPDGPADL
                     AFLIAAPEAGGAEHMKLLSSLARALVRKEFVASLREASTPDEVVGLIDEVLNPGDKPK
                     PQAPVAAPAADKPKTLVAITACPTGIAHTYMAADSLVAAAKDAGVTLHVETQGSSGST
                     PLAASTIVGADAVIFATDVGVKDKGRFAGKPVVASGVKRAINEPAKMVAEALAAANNP
                     NAARVEGSGADALGSAAPAGGVGWGTRTRQILLTGVSYMIPFVAAGGLLIALGFLFGG
                     YEIANKPAGGTDSFAHMYALNNSLWNLPPGGVSQYLGAVLFSLGGLAFSFLVPALAGY
                     IAFAIADRPGIAPGFTAGAVAVFVGAGFIGGIVGGLIAGFAALWISKRPVPKWFRGLM
                     PVVIIPLFASLVVGLLMFMLLGRPLAALTTGLTNWLSGMSGTSAVLLGVILGLMMCFD
                     LGGPVNKAAYAFATAGLTAATTASFEIMAAVMAAGMVPPLAMALATFVRPQLFSEPER
                     ENGRAAVLL"
BASE COUNT          452 a         1074 c          977 g          465 t
ORIGIN      
        1 ggcaccaacg gcatcagcgt caggcacggc ctgtccaccc cggacagcga agaggccgcc
       61 gtaaagcgcg caatgatcgc cagcgccaac tacgttgtgg tggccgctga ttcatcgaag
      121 gtcggccgtg aggacttcat cagcttcgcc ccgctgtcga gcgtcgacgc actcatcacc
      181 gactccgaga tcagcggagc gtaccgggaa cagctcaccg agcagggcat cgaagtagtc
      241 gtcgcatgat cgtcaccgtc acgccgaacc cgagcctcga ccgcaccgtc acgctggcca
      301 ctccactcac ccgaggtgcg gtgcaccggg tcaattcggt gaccaccgag ccgggcggca
      361 agggcgtcaa cgtcgcgcgc gccctggccc tcgcaggcgt cgacgccgtc gcgctgctgc
      421 ccgcgtcggg taccgatccg atggtcaccg cgctgcagag catcggagtg cctttccgct
      481 gcgtcccgat cacgtcgccg gtgcgcacca acatcgcggt caccgaaatg gacggcacca
      541 caacgaagat caacgagccc ggcgcgagat tggacggcgc cgcggtcgac gcgctcaccc
      601 ggtccgtcgt cgccaatgcc gggcacgcct catgggtggt gatgtcgggc tcgctgccgc
      661 cggggatgcc cgagtcgtgg tacgccgacg tcgtcgcaca actcgcaccc tacgactgca
      721 aggtcgcagt cgatacgtcg gaccgaccgc tggcggcact ggtcgacggc ttcgatcggg
      781 ccgcgcccga cctgatcaaa cccaacgccg aagagctcgc aggagtcatc ggcttcgccc
      841 cgcaggcgct ggaagctgcg ctgtcacaag gtgatccaga gcccgtggtg tcggccgcgc
      901 gccagctgct tgaccggggt gtcggcgcgg tactggccac gctgggcgcg gcgggagccg
      961 tcctcgtgac cgacaccggc agctggctgg cgactccgcc gccgatcctc ccgcgcagca
     1021 cggtcggtgc gggcgactcc agcctggcgg gatatctgcg tgccgatgtg ggcggggccg
     1081 agccggcgca acgactgcag atggccgtcg cctacggcag cgcagcggcc gccctacctg
     1141 gctccgcact gcccactcct agtgagatcg acctcgatga cgttgtggtg acgtccatcc
     1201 cgctgatcac caacccctcc taacaaccaa cgaaggtatc gccatgacca gcaccgcttc
     1261 ccgacccatc atcgccgtcg acctcgttct tctcgacgtc gacgcgggcg ccgacaagca
     1321 ggccgtgatc gggcgtctcg tcggcagact cgccgacgcc ggacgcacca ccgaccccga
     1381 cgggctgatc gccgcggcga tggcccgcga agagcagtcg gcaaccgggc tgcccggcgg
     1441 catcgcgatc ccacactgcc gctcaccgca cgtcgacacg gcgtccgtcg gcttcgcgcg
     1501 gctgcatccg cccgtcgact tcggcgcacc cgacggcccg gcggatctgg cgttcctcat
     1561 cgccgcaccc gaggccggcg gcgccgagca catgaagctg ctctcgagcc tggcccgcgc
     1621 gctggtgcgc aaggagttcg tcgcatcgct gcgcgaggca tccacacccg acgaagtggt
     1681 cgggctgatc gacgaggtgc tgaaccccgg cgacaagccg aagccgcagg caccggtggc
     1741 ggcgcctgcc gccgataagc cgaagacgct ggtggcgatc accgcgtgcc ccaccggaat
     1801 cgcgcacacc tatatggccg ccgactcgtt ggtcgccgcg gcgaaggacg caggcgtcac
     1861 cctgcatgtc gagacgcagg gcagctcggg cagcaccccg ctggccgcct cgacgatcgt
     1921 cggcgccgac gccgtcatct tcgccactga cgtcggggtg aaggacaagg gccgttttgc
     1981 gggcaagccg gtggtggctt ccggtgtcaa gcgcgcgatc aacgagccgg ccaagatggt
     2041 cgccgaagcg cttgccgcgg cgaacaatcc gaatgccgcc cgggtcgagg gctccggcgc
     2101 cgacgcgctc gggagcgccg cacccgccgg tggtgtcgga tggggcacca ggacccgcca
     2161 gatcctgctg accggcgtca gctacatgat cccgttcgtc gcggccggtg gtctgctgat
     2221 cgcgcttggc ttcctcttcg gcggctacga gatcgccaac aaacccgccg gcggaaccga
     2281 ctccttcgcg cacatgtacg cgctcaacaa ctcgctgtgg aatctgcctc caggcggcgt
     2341 ttcgcagtac ctcggcgcgg ttctgttcag cctcggtggc ctcgcgttca gcttcctggt
     2401 acccgcactg gccggctaca tcgccttcgc gatcgccgac cggccgggta tcgcacccgg
     2461 tttcaccgcc ggagcggtgg ccgtgttcgt cggcgcgggg ttcatcggcg gcatcgtcgg
     2521 cggtctgatc gccggcttcg cagcgctgtg gatcagcaaa cggcctgtgc ccaagtggtt
     2581 ccggggcctg atgccggtgg tcatcatccc gctgttcgct tccctcgtcg tcggcctctt
     2641 gatgttcatg ctgctcgggc gcccattggc cgcactgacc accggcctga ccaactggct
     2701 cagcggaatg tcgggcacct ccgcggtcct gctcggcgtc atcctgggcc tgatgatgtg
     2761 cttcgacctc ggcggcccgg tcaacaaggc ggcctacgcg ttcgccaccg cggggctcac
     2821 cgccgcgacc acggcgtcgt tcgagatcat ggcggcggtg atggctgcag gcatggtgcc
     2881 gccgctggcg atggcgctcg cgacattcgt ccggcctcag ttgttcagcg agccggagcg
     2941 ggaaaacggc cgggccgctg tccttctc
//
LOCUS       DASYLX010000115         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1760649,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000115 DASYLX010000000
VERSION     DASYLX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760649"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGP27_02740"
     CDS             <1..349
                     /locus_tag="VGP27_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850267.1"
                     /translation="GPTHPSNLNCKCRKHHLMKTFWTGIGGWKDEQLPDGTLIWTSPS
                     GKTYTTHPGSRLFYPTWNVTTAELPPPTAPPPPDTQHRGLMMPRRKRPRAADIAAAIQ
                     AERALNQHDTPPF"
     gene            433..930
                     /locus_tag="VGP27_02745"
     CDS             433..930
                     /locus_tag="VGP27_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7850268.1"
                     /translation="MSAHAVDQLRFRAVDQNDPLAEPLLAELAQEYASRYRAPVEGVA
                     KWLRTYPADEFAAPGGAMLIGLFDGRPVTGGAFRRFDAETAELKRIWTDSRYRRRGYA
                     KALLAELEKEIAARGYRRIYLTTGDRQPEAEELYVSTGYRRLDEPLPAEGEVYPLAFL
                     KVLTP"
     gene            927..2015
                     /locus_tag="VGP27_02750"
     CDS             927..2015
                     /locus_tag="VGP27_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7850269.1"
                     /translation="MSDHMHLAVALDGHGWHPEAWRQSARTEPVTSGRYWSELAATAE
                     RGLLDFVTFDDALTPQRRRRPEIDSRWLAGRPDAVLVASRVAPVTRHIGLIPVATVTH
                     TEPFHISKAIATLDFVSHGRAGWQVRISSSPHEAELFGRREVTGVDLFDEADDVVEVV
                     RRLWDSWEDDAVIREVETGRFVDRDKLHYIDFTGKHFSVKGPSITPRPPQGQPVVAAL
                     AHQLPVYEFAARSADLVFITPQDDDSVREIVGQVRRAGGTDLKLYADVFVTFSGTVDA
                     RSDAFVFDGTPADLVDLLARWQQLGLDGFRLRPSVNATDLPAIVDEVVPLLQRGNLFR
                     TAYRDGEPLRQRLGLPVADNRYAKVEPS"
     gene            2012..>2341
                     /locus_tag="VGP27_02755"
     CDS             2012..>2341
                     /locus_tag="VGP27_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7850270.1"
                     /translation="MTVPLSILDLSPISEGSDAATALHNTVDLAQHAEQWGYKRYWLA
                     EHHFAAVASSQPALLIGQMASATQHIHVGAGAVQLGNTTAPAVVESFGILDAFYPGRI
                     DLGVGRSA"
BASE COUNT          413 a          850 c          716 g          362 t
ORIGIN      
        1 ggggccgaca cacccgtcga acctgaactg caaatgccga aaacaccacc tgatgaaaac
       61 gttctggacc ggcatcggcg gatggaaaga cgaacagctg cccgacggca ccctcatctg
      121 gacctcgccc agcggcaaga cctacaccac ccaccccggc agccgactgt tctaccccac
      181 ctggaacgtc accaccgccg aactaccccc accgactgca ccaccacccc ccgacaccca
      241 acatcgcggc ctgatgatgc cgcgccgaaa acgcccccgc gccgccgaca tcgccgcggc
      301 aattcaggcc gaacgcgcac tcaaccaaca cgacacccca ccgttttagc gacctgcggg
      361 tatttcgaat cagcctgacc gaaatcaggt ccgaggaatg cgcgtttgcg aactgcgtta
      421 gacagttaag tgatgtccgc gcacgccgtt gatcagctcc gctttcgggc ggtcgaccag
      481 aatgacccgc tggccgaacc gctgctggcg gaacttgcgc aggagtacgc cagtcgatat
      541 cgcgctccgg tcgagggggt agccaaatgg ctgcgcacct atccggccga cgagttcgcg
      601 gcacccggcg gagccatgct gatcggcttg ttcgacgggc gacccgtcac cggcggagcg
      661 ttccggcggt tcgacgccga aaccgcggaa ctcaagcgca tctggaccga tagccgatac
      721 cggcggcgcg gctacgcgaa ggcgttgctg gcggagctgg aaaaggaaat agccgcccgc
      781 ggctaccgcc gcatctacct cacgacgggc gatcgccagc ccgaggccga agagctgtac
      841 gtgtccacgg gctaccgcag gctcgatgag ccgctacccg ccgagggcga ggtctatccg
      901 ctcgcgttcc tgaaggtgct gacgccatga gcgaccacat gcacctcgcg gtcgcgctgg
      961 acggacacgg ctggcatccc gaggcgtggc ggcagtcggc gcgcaccgag cccgtcacca
     1021 gcggccgcta ctggtccgaa ctcgccgcca ccgccgaacg cgggctgctg gacttcgtga
     1081 ccttcgacga cgcgctcacg ccgcagcggc ggcgacgtcc cgaaatcgac tcacggtggt
     1141 tggcgggccg acccgacgcc gtccttgtcg cctcacgggt cgcgcccgtc acccgtcaca
     1201 tcggactcat cccggtcgcc accgtcactc acaccgagcc gttccatatc tccaaagcca
     1261 ttgccaccct tgacttcgtc tcccacggcc gggccggttg gcaggtgcgc atcagcagtt
     1321 cgccgcacga agccgagctc ttcggccgtc gcgaggtcac cggggtcgac cttttcgacg
     1381 aggccgacga cgtcgtagag gtggttcggc gactgtggga cagctgggag gacgacgcgg
     1441 tgatccgcga ggtcgagacg gggcgattcg tcgaccgcga caagctgcac tacatcgact
     1501 tcaccgggaa gcacttctcg gtcaaggggc cgtccatcac cccgcggcca ccccagggcc
     1561 aaccggtcgt cgccgccctc gcgcatcaac tgccggtcta cgaattcgcc gcgcgcagtg
     1621 ctgatctggt cttcatcacc ccgcaggacg acgactccgt gcgcgagatc gtcgggcagg
     1681 tcaggcgggc cggcggcacc gatctcaagc tgtacgccga cgtcttcgtc acgttctccg
     1741 gaaccgtcga tgcccggtcg gacgcgtttg tcttcgatgg cacccctgcc gatttggtcg
     1801 atctccttgc ccgttggcag cagctgggcc tcgacggctt ccggttgcgg ccttcggtga
     1861 acgccaccga cctgccggcg atcgtcgacg aggtggtgcc gctgctgcag cgcgggaatc
     1921 tgtttcgcac cgcttaccgc gacggggagc ctctacggca gcggctcgga ctgcccgtcg
     1981 ccgacaaccg ctacgccaag gtggagccgt catgaccgtc ccgctgtcga tcctcgactt
     2041 gtcgccgatc agcgaaggca gcgacgccgc aaccgcgctg cacaacaccg tcgacctcgc
     2101 gcagcacgcc gaacagtggg gctacaagcg ctactggctg gccgagcacc acttcgccgc
     2161 tgtcgcgagc tcgcaacccg ccttgctgat cgggcagatg gcctcggcca cccagcacat
     2221 ccacgtcggc gcgggcgcag tgcaattggg caataccacc gcccctgcgg tcgtcgagag
     2281 tttcggcatc ctcgacgcgt tctaccccgg ccgcatcgac ctaggtgtgg gcaggtcggc
     2341 g
//
LOCUS       DASYLX010000116         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_663761,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000116 DASYLX010000000
VERSION     DASYLX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663761"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VGP27_02760"
     CDS             <1..416
                     /locus_tag="VGP27_02760"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HEV7850271.1"
                     /translation="ITFFTNYDSAKGEQLASTPYASATFPWFALGRQVHVRGPVTRVP
                     AEVTADYWRHRPRGSQLGAWASHQSHPIESRAALLDQLADVTQRFAGLDEVPVPPNWG
                     GYLVAPDVVEFWQGRENRVHNRIRVAEGRVQRLQP"
     gene            416..1741
                     /locus_tag="VGP27_02765"
     CDS             416..1741
                     /locus_tag="VGP27_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850272.1"
                     /translation="MAGFFADVTPLRTSPDFRRLFSAGVVTVVGANLTIFAVPVQIYA
                     LTQNSAYVGLSGLFALVPLVVFGLWGGAWADAMDRRLLLIITSCGLAVASVLLWVQAA
                     LSLNNVWVVLCLLSVQQAFYAINQPTRSAAIPRMVAADQLPAANSLNITVFQFGAIVG
                     PLMAGVMLRWVDLSTLYLIDTITCVAPIWATFRLKPIPPSNGQAGSSGWGFRAVLDGF
                     RYLAGNTVVLMSFVVDLIAMIFGMPRALFPQMAHESFGGPVEGGTTMALLAAAISVGA
                     VAGGVFSGWLPRIQRQGLAVVLAILVWGAAMIGFGAASGLAGGTAGLMLWIALVFLAI
                     GGAADMVSSAFRNTILQEAASDDVRGRLQGVFTVVVAGGPRLADAVHGAAAAAVGTTI
                     AAAGGGGLVVVGVLVAAVLAPVFVRYRVSRPRTGGPKLASELTPDDDKV"
     gene            1808..2458
                     /locus_tag="VGP27_02770"
     CDS             1808..2458
                     /locus_tag="VGP27_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850273.1"
                     /translation="MPGDSWDSAAIGLRERKKQRTRATLIDAAIELCEAQGFEQTTVE
                     QIAAIADVSPRTFSRYFATKEAVCLALVDDAVDVTAVELGRLPGDMNHLEALYRASVT
                     MYRNTKNAGIGELTAPRLMCTLRIIMSSQTLRQATMEFRPHALNVELARRMDVELEDR
                     GLQLVAAVWASIIMTALADLGMDTRWQGVDIDDAVDRIEATFDQFAGLIDGVAQPV"
     gene            2549..3844
                     /locus_tag="VGP27_02775"
     CDS             2549..3844
                     /locus_tag="VGP27_02775"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV7850274.1"
                     /translation="MAEKDEHASLRYPGGEIDLDIVHATEGSDGIALGSLLAKTGYTT
                     FDEGFVNTASTKSAITYIDGDAGILRYRGYPIEQLAEKSSFIEVAYLLIYGELPTKDQ
                     LDTFTGQIQRHTLLHEDLKRFFDGFPRNAHPMPVVSSAVNALSAYYQDSLDPFDDEQV
                     ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLTLVENFLRMTFGFPAEPYEVDPEV
                     VRALDLLLILHADHEQNCSTSTVRLVGSSQANLFTSISGGINALWGPLHGGANQAVLE
                     MLEKIRQSDGDVHDFVKRVKNREDNVKLMGFGHRVYKNYDPRARIVKEQADKILGKLG
                     GDDELLDIAKALEEVALTDDFFVERKLYPNVDFYTGVIYRAMGFPTRMFTVLFSVGRL
                     PGWIAHWREMHEDGSGKIGRPRQIYTGYTERDYTDLGKR"
     gene            3985..>4454
                     /locus_tag="VGP27_02780"
     CDS             3985..>4454
                     /locus_tag="VGP27_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850275.1"
                     /translation="MNETVVRSLSARRKAIILVTCCLSLLIVSMDATIVNVAIPSIRA
                     EFGASASQIQWVIDIYTLVLASLLLLSGATGDRFGRRRTFQIGLTVFAVASLMCSLAP
                     SIEMLIGARFLQAIGGSMMNPVAMSIITQVFRGRVERARAIGVWGGVVGISMALG"
BASE COUNT          724 a         1434 c         1454 g          842 t
ORIGIN      
        1 ggatcacctt cttcaccaat tacgactccg ccaagggtga gcaactcgcc tcgacgccgt
       61 acgcgtcggc gaccttcccg tggtttgcgc tcggccgcca ggtgcacgtc cgcggcccgg
      121 tgacgagggt gcccgcggag gtgaccgccg actactggcg ccatcggccg cgcggctccc
      181 agctgggcgc ctgggcttcg caccaatctc atccgatcga gtcgcgcgcg gctctgctcg
      241 accagttggc cgacgtcaca caacgtttcg ccggcctcga cgaggtcccg gtgccgccga
      301 actggggtgg atacctcgtc gcgccggacg tcgtcgaatt ttggcagggc cgggagaacc
      361 gggtgcacaa caggattcgg gtagcagagg gccgcgtcca gcggcttcag ccgtagtggc
      421 ggggttcttc gccgacgtca ctccgctgcg gacctcgccg gacttccgac ggctgttctc
      481 ggccggcgtc gtcacggtcg tcggggccaa cctcaccatc ttcgcggtgc cggtccagat
      541 ctatgcgctc acccagaact cggcgtacgt cggtctttcc ggcctgttcg cgctggttcc
      601 cctcgtcgtg ttcggcctgt ggggaggcgc ttgggcggac gcgatggatc gacgcctgct
      661 actgatcatc acctcctgcg ggctggccgt ggcatcggtg ctgctgtggg tgcaggctgc
      721 actgtcgctc aacaacgtgt gggtggtgct ctgcctgctg tcggtgcagc aggcgttcta
      781 tgcgatcaat cagccgacgc gttcggcggc gatcccacgg atggtggccg ccgaccaact
      841 gcctgcggcc aactcgctca acatcacagt gttccagttc ggcgcgatcg tcggcccgct
      901 gatggcgggg gtcatgctgc gctgggtcga cctgtccacc ttgtacctga tcgacacgat
      961 cacctgtgtc gcacccatct gggccacgtt ccggctcaag ccgattccac cgagcaacgg
     1021 ccaggccggc tcgtcgggct ggggattccg tgccgtgctc gacgggttcc gctatctggc
     1081 aggcaacacg gtcgtgctga tgtcgttcgt cgtcgacctg atcgcgatga tcttcggcat
     1141 gccgcgggcg ctctttccgc agatggctca cgaaagcttc ggcggacccg tcgagggcgg
     1201 caccaccatg gcgctgctgg ccgccgccat ctcggtgggc gcagtggcgg gcggcgtgtt
     1261 ctccggctgg ctaccgcgca ttcagcgcca gggcttggct gtggttctcg cgatcctggt
     1321 gtggggcgcg gcgatgatcg gcttcggtgc ggccagtggt ctggcgggcg gaacggccgg
     1381 gctgatgttg tggattgcct tggtgtttct cgctattggc ggtgcagcgg acatggtgtc
     1441 gtcggcgttc cgcaacacaa tcctgcaaga ggcggcatcc gatgatgtcc gcggccggct
     1501 gcaaggggtg ttcaccgtcg tcgttgcggg cggtccccgc ctcgccgacg cggtgcacgg
     1561 cgccgccgcc gcggccgtcg gcacaacgat cgccgccgcc ggtggcggag gactggtcgt
     1621 cgtgggggtt ctggttgcgg cggttctggc gcccgtattc gtgcggtacc gggtcagccg
     1681 gccccgcacc ggcggtccaa aattagccag cgaacttacg ccggatgacg acaaagtctg
     1741 attgcggcag attacgatcc aagccacttt ccttggcaac ttcaagtggc cgagttaaca
     1801 tcctggcgtg cctggtgatt cgtgggattc ggcagcaatt ggtttgcgcg agcgtaagaa
     1861 acagcgcacg cgcgccacgc tgatcgacgc ggccatcgag ctatgcgaag cgcaggggtt
     1921 tgagcaaact accgtcgagc agatcgccgc gatcgccgac gtctcacccc gcaccttcag
     1981 ccgttacttc gccaccaagg aagcggtgtg tctggctctg gtcgacgacg ccgtcgacgt
     2041 gaccgcagtg gaattgggcc gcctgcccgg ggacatgaat cacttggagg cgctgtaccg
     2101 cgcgtcggtg acgatgtatc gcaacaccaa gaacgcaggc atcggcgaac tgacggcccc
     2161 gcggctgatg tgcacgctgc gcatcatcat gtcctcacag acattgcggc aggcgaccat
     2221 ggagttccgg ccgcacgcgc tcaacgtcga gctggccagg cggatggacg tcgaactcga
     2281 agaccgaggg ctgcagctgg tcgccgcggt gtgggcgtcg atcatcatga ccgcgctggc
     2341 cgatctgggc atggacaccc gttggcaggg cgtcgacatc gatgacgcgg tcgaccggat
     2401 cgaagcgacg ttcgaccagt tcgcaggcct gattgacggc gtcgcgcagc cggtctgagc
     2461 ctgcgcgcac cccgcgcggg cgcgattgcc acaatgggac taccttttga gggcagaggt
     2521 cggttctctc agtgacgaag ggatgcctgt ggcagaaaaa gacgagcacg ccagcctgcg
     2581 ctatcccggt ggcgagattg atttggatat cgtccacgcc acggagggtt ccgacggtat
     2641 cgcgctgggg tcgctgctgg cgaagaccgg ctacaccacc ttcgacgagg gcttcgtcaa
     2701 caccgcctcc accaagagcg ccatcaccta catcgacggt gacgcgggca tcctgcgcta
     2761 ccgcggatat ccgatcgagc agctcgccga gaagtcaagc ttcatcgagg tcgcctatct
     2821 gctgatctac ggcgaactgc cgacgaagga tcagctggac accttcaccg ggcagatcca
     2881 gcggcacacg ctgctgcacg aggacctcaa gcggttcttc gacggctttc cgcgcaacgc
     2941 acatccgatg ccggtggtat cgagtgcggt taatgcgctg tcggcgtact accaggattc
     3001 gctggatccc ttcgacgacg aacaggtcga gctgtccacg atccggttgc tggccaaact
     3061 gccgaccatc gccgcgtacg cctacaagaa gtcggtcggc cagccgttct tgtatcccga
     3121 caattcgctg acgctggtcg agaacttcct gcggatgacg ttcggcttcc ccgccgagcc
     3181 ctacgaggtg gacccggaag tcgtgcgggc acttgacctg ctgctcatcc tgcatgccga
     3241 ccacgagcag aactgctcga cgtcgacggt gcgtctggtc ggctcgtcgc aggccaacct
     3301 gttcacctcg atctccggcg gcatcaacgc gctgtggggt ccgctgcacg gcggcgccaa
     3361 ccaggctgtg ctggaaatgc tggagaagat ccgccagtcc gacggcgacg tgcacgactt
     3421 cgtcaagagg gtcaagaacc gcgaagacaa cgtgaagctg atgggcttcg ggcaccgggt
     3481 gtacaagaac tacgacccgc gggcgcgcat cgtcaaggag caggccgaca agatcctggg
     3541 caagctcggc ggcgacgacg aactgctcga catcgccaag gccttggaag aggttgcgct
     3601 caccgacgac ttcttcgtcg agcgcaagct gtatccgaat gtcgacttct acaccggtgt
     3661 gatctaccgc gcgatgggct tcccgacccg gatgttcacc gtgctgttct ccgtcggccg
     3721 gttgccgggc tggatcgctc actggcggga gatgcacgag gacggcagcg gcaagatcgg
     3781 ccgcccgcgc cagatctaca ccggctacac cgagcgcgac tacaccgacc tcggcaagcg
     3841 ctaaccccca ctccgcccaa accaacgttt gggcggactt ttgcgagtgg atcgcgccat
     3901 ttcgtccatc tcggcggcga cctgagaggc atttcactgt catccgcctt gcccgaacaa
     3961 cagttgtagt ttcacaatag ttatgtgaat gaaaccgtcg tccgatcact gagcgctcgc
     4021 cgcaaggcca tcatcttggt gacctgctgt ctgagcctgc tgatcgtctc gatggatgcg
     4081 accatcgtca acgtcgcgat ccccagcatc cgcgccgagt tcggcgcgtc cgcgtcgcag
     4141 attcaatggg tcatcgacat ctacacgctg gtcctggcct cgctgctgct gctgtccggc
     4201 gcgaccggcg accggttcgg ccgtcggcgc acattccaga tcggcctgac ggtcttcgcg
     4261 gtcgcgtcgt tgatgtgcag ccttgcgccg agcatcgaga tgctgatcgg cgcccgcttc
     4321 ctgcaggcca tcggcgggtc gatgatgaat cccgtcgcga tgtcgatcat cacccaggtg
     4381 ttccgggggc gggtggaacg cgcacgggcg atcggcgtct ggggtggggt cgtcggcatc
     4441 tcgatggcgc tcgg
//
LOCUS       DASYLX010000117         6585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_224914,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000117 DASYLX010000000
VERSION     DASYLX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224914"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VGP27_02785"
     CDS             <1..545
                     /locus_tag="VGP27_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7850276.1"
                     /translation="DETLLGGDNSAAAVTGYGPIPAAVARRLVGRAVTDKRSRCTLRR
                     LYRHPKTGQLVAMESRSRFFPKGLARFIGLRDQTCRTPYCDAPIRHRDHARPRHRDGP
                     TSAENGLGECEHCNYVKESPGWQVTAGSDNNGVHTAVFQTPAGARYTSTAPPLLAMPH
                     IEIAETEVRIGIAIAQQDAA"
     gene            complement(548..1333)
                     /locus_tag="VGP27_02790"
     CDS             complement(548..1333)
                     /locus_tag="VGP27_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding protein"
                     /protein_id="HEV7850277.1"
                     /translation="MSEREPDFGQPWAPHRRRIYLAVAAFTGLVFGVMTVGVGLGLIE
                     PTFTSDVICNLLFGVPVILLPLVLLWDAPGEERSRLDKAAELTLFYLPYTAFSQIGYE
                     LMFLIGSPLGWWGPTSDPGFKWLWWQYGLADTRYVSGNPWTFGLEVVGVTTGIVVITV
                     WMRLIRRNLAAESRIRCLWVAFACCAVLMSSTAVYFLSEVGAGLNDIGQGAFGLWFKF
                     VGENVPFVVLPPLVLYAIYLQIDYLTRRAGVRPRHPQAVSQDA"
     gene            1440..2705
                     /locus_tag="VGP27_02795"
     CDS             1440..2705
                     /locus_tag="VGP27_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7850278.1"
                     /translation="MARFPKPAEGSWTQHYPQLGTEPVSYEDSISPEFYEAERKAVFK
                     RAWLNVGRVEQLPRKGSYFTKELKVVNTSIILVRNSAGEVKAFHNICRHRGNKLVWND
                     MPLEETSGFCKQFTCKYHAWRYDLDGNLKFVQQEGEFFDLDKSRYGLVPVHCDVWEGF
                     IFVNFAPDPEQNLRDFLGPMVTGLEGYPFDRMTSRWFYRSEVKANWKLYMDAFQEFYH
                     APVLHANQSPAKYSKAAAEAGFEAPHYRIDGPHRLVSTSGVRAWEMDSEMRKPMEDIC
                     QSGLFGPWDSPDLGEMPVGLNPAKCEPWGLDSFQLFPNFVILIWGQGWYLTYHYWPTS
                     HNTHIFEGTLYFPAPRTPRERIAQELAAVSFKEYGLQDANTLEATQTMVESQVVDRFL
                     LCDQEVLIRHLHQETAAWVDDYHNKTAGV"
     gene            2705..3040
                     /locus_tag="VGP27_02800"
     CDS             2705..3040
                     /locus_tag="VGP27_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850279.1"
                     /translation="MAVTAMLPAEFTDLERFSDWCLASEAERYAKRMATSMAQMQAFY
                     DAIAPRAEEAISFCDKFPLDDLPDDVLNLMHLLYSMIMVSFPVECWKQPRVPDSGAAA
                     LDCLSEPVP"
     gene            3037..4278
                     /locus_tag="VGP27_02805"
     CDS             3037..4278
                     /locus_tag="VGP27_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850280.1"
                     /translation="MTKKTVLRAERWADVEAGEVRSPAVVVIDGERVQAVNPAELPAD
                     STEIDLGDVTLVPGLMDMELNLLIGGPGGPDGLPSPMHGVQDDPAYRTLRGAVNAKTT
                     LEAGFTTVRNLGLMVKTGGYLLDVALQRAIEARWHVGPRIYPAGHAVTPYGGHLDPTV
                     FQRLAPGIMPLSVAEGIANGVDDVRACVRYQIRHGAKLIKVSASGGVMSHSTAPGAQQ
                     YSDDEFVAIADEAHRAGVRVAAHAVGDSAIRACIRAGIDCIEHGFLASDETIQMMVDH
                     DTFLVSTTYLTEAMAVDRIAPELRKKAEEVFPRAQAMLPKAIAAGVRIACGTDAPAIP
                     HGQNAKELCALVDRGMTPMQALRAATITSAELIEADEELGRLAPGYLADIIAVPGDPS
                     RDIATTLDVRFVMKNGVVYKQ"
     gene            4311..4766
                     /locus_tag="VGP27_02810"
     CDS             4311..4766
                     /locus_tag="VGP27_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7850281.1"
                     /translation="MEQTDNILWLLKQAWYFSLTTVNDAVSEHGVSTAQIGMLRQLAT
                     EPGLSGADLARRLLISPQGVQLALKELERRGLVERKRDPQHGRILQAFLTGQGRKVAE
                     AVVSDAIAAHAKVFDVLTSDEQETLRELLGRVIEQGTGHKLHTDHIGPE"
     gene            4851..5126
                     /locus_tag="VGP27_02815"
                     /pseudo
     CDS             4851..5126
                     /locus_tag="VGP27_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     gene            5131..5874
                     /locus_tag="VGP27_02820"
     CDS             5131..5874
                     /locus_tag="VGP27_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850282.1"
                     /translation="MSRVAVVTGGASGMGEATCHELGRRGHKVAVLDVDAHAAQRVSE
                     NLRADGVTALAVAADVSDRATVEEAFAKVRTELGPVQILVTSAGAVDFAPFTEITIEN
                     WERLIAVNLTGTFHCCQVAVPDMVEAGWGRIVMISSSSAQRGSPKMAHYAASKGALLS
                     LTKSLAREYGSVGITVNNVPPSGIETPMQHQGQAAGHLPSNEQMAAGIPVGHLGTGDD
                     IAAAVGFLCSEQAGFITGQVLGVNGGQVL"
     gene            5893..>6585
                     /locus_tag="VGP27_02825"
     CDS             5893..>6585
                     /locus_tag="VGP27_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7850283.1"
                     /translation="MAKWPKPAEGSWTEHYPELGTGPISFRDSTSPQFYELEREAIFK
                     RAWLNVGRVEEVPRVGSYFTKEIDVAKTSVIVVKGKDQQIRAFYNICRHRGNKLVWND
                     FPNEEVRGACRQFTCKYHGWRYDLEGALTFVQQPGEFFDLDDKHYGLSPVHCDVWNGF
                     IFINLDPEPRQSLREFLGPMITALDDYPFEKMTERYDFVAHNNSNWKIFADAFQEYYH
                     VPSLHSQQVPSEV"
BASE COUNT         1305 a         2065 c         2143 g         1072 t
ORIGIN      
        1 ccgacgagac gctgctgggc ggcgacaaca gcgcggccgc ggtcaccggg tacgggccga
       61 tcccggcggc ggtggcgcgc cggctggtcg gtagggcggt caccgacaag cggtcacgat
      121 gcacgctgcg ccggctctac cgccacccca agacgggcca gctggtggcg atggagtcgc
      181 ggtcgcggtt cttccccaaa ggattggcgc gcttcatcgg gctgcgcgac caaacctgcc
      241 gcaccccgta ctgcgacgcg ccgatccgac atcgcgacca cgcccggccc cgccaccgcg
      301 acggaccgac cagcgccgaa aacgggttgg gcgaatgcga acactgcaac tacgtcaagg
      361 agtcaccggg ctggcaagtc acggccggca gcgacaacaa cggtgtgcac acagcggtat
      421 tccagacacc ggccggcgcc cgctacacat cgacggcgcc gccactgctg gcaatgccac
      481 acatcgaaat agccgaaacc gaagtcagga tcggcatcgc catcgcccaa caagacgccg
      541 cctaggtcta ggcgtcttgc gagacggctt gcggatgccg cggccgcacc cccgctcttc
      601 tggtgaggta gtcgatctgc agataaatcg cgtagaggac gagcggcgga agaaccacga
      661 acggaacgtt ctcgccgacg aacttgaacc agaggccgaa ggcaccctgc ccgatgtcgt
      721 tgaggccggc gccgacctcg gacaggaaat acaccgcggt actgctcatg agcacggcgc
      781 agcaggcaaa ggccacccac agacaccgaa tgcgcgactc cgccgcgaga ttgcgccgga
      841 tcagtcgcat ccacaccgtg atgaccacga tgccggtggt gacaccgacg acctccagcc
      901 cgaaggtcca cggattgccg ctgacatagc gggtgtcggc cagtccgtat tgccaccaca
      961 gccatttgaa accgggatcc gatgtcggac cccaccagcc gagcggagac ccgatgagaa
     1021 acatcagctc gtagccgatc tggctgaagg cggtgtaggg cagatagaac aaggtcagtt
     1081 cggccgcctt gtcgagccgg gaacgctctt ctcccggcgc atcccagagc aggaccagcg
     1141 gaagcaatat caccggcacg ccgaagagca ggttgcagat gacgtcggaa gtgaacgtgg
     1201 gctcgatcag cccgaggccg accccgaccg tcatgacgcc gaacaccaaa cccgtgaagg
     1261 ccgccaccgc gagatagatc cgcctgcggt gtggcgccca cggctgtccg aagtcgggtt
     1321 ctcgttcgga catcgctgtc acgctccctg cctgaagtaa gagcaatatc aactacttgc
     1381 gattgtgggc ggaagcatat accgtcgggc cgtcagccat ccgcagaatg gagtaggcaa
     1441 tggcgcgatt ccccaaaccg gcagaaggca gttggacgca gcactatccg caactgggaa
     1501 ccgagccggt gtcctacgag gactcgatca gcccagagtt ctatgaggcg gagcgcaagg
     1561 cagtgttcaa acgggcatgg ctgaatgtcg gtcgtgtaga acaacttcca cgcaagggga
     1621 gttacttcac caaagagctc aaggttgtca acacctcgat catcctggtg cgcaacagcg
     1681 ccggtgaggt gaaggcgttc cacaacatct gcaggcaccg tggcaacaag ctggtctgga
     1741 acgacatgcc gctcgaggag accagcggct tctgcaagca gttcacctgc aaataccacg
     1801 cctggcggta cgacctcgat ggcaacctga agttcgtcca gcaggaaggt gagttcttcg
     1861 acctcgacaa gagccgctac gggttggtcc ctgtgcactg cgatgtgtgg gaaggcttca
     1921 tcttcgtcaa cttcgcgccc gaccccgagc agaacctgcg cgacttcctc gggccgatgg
     1981 tcaccggact ggagggctat ccgttcgatc gaatgacgtc gcgctggttc taccgctcag
     2041 aggtcaaggc gaactggaag ctctacatgg acgcgttcca ggagttctat cacgcacccg
     2101 tattacacgc gaaccagtcg cccgcgaagt actcgaaggc ggccgcggag gcgggtttcg
     2161 aggcaccgca ctaccgcatc gacggcccgc accggttggt cagcacatcg ggtgtccggg
     2221 cgtgggagat ggattccgag atgcgcaagc cgatggagga catctgccag agcggcttgt
     2281 tcggtccgtg ggactcaccc gatctgggcg agatgcccgt cgggctcaac ccggcgaaat
     2341 gtgagccctg gggcctcgat tcgtttcagc tgttcccgaa cttcgtgatc ctgatctggg
     2401 gtcagggctg gtatctgacc tatcactact ggccaacctc gcataacaca cacatcttcg
     2461 aaggcacgct gtatttcccg gcgccccgca cgccgaggga acgcatcgcc caggaactgg
     2521 ccgcggtgtc gttcaaggag tacggcctgc aggacgccaa cacgctggag gcgacccaga
     2581 cgatggtgga atctcaggtc gtggaccggt tcttgctctg cgaccaggag gtgctcatcc
     2641 gccacctgca ccaggagacc gcggcgtggg tcgatgacta ccacaacaag acggcgggcg
     2701 tgtgatggcc gtgaccgcga tgctgccggc cgaattcacc gatctggaaa ggttttccga
     2761 ctggtgcctg gcgtccgagg cggagcggta tgccaagcgg atggccacct cgatggccca
     2821 gatgcaggcg ttctacgacg ccatcgcgcc gcgtgcggag gaggcgatct ccttctgcga
     2881 caagtttccg ctcgacgatc tgcctgacga cgtcctcaac ctgatgcacc tgctgtactc
     2941 gatgatcatg gtgtcgttcc ccgtcgagtg ctggaagcag ccgcgggtgc ccgactccgg
     3001 tgcggccgcg ctggattgtc tttccgaacc cgtgccatga cgaagaagac cgtcctgcgc
     3061 gccgagcgct gggccgacgt cgaggcaggc gaggtgcgct cgcctgcggt ggtcgtgatc
     3121 gacggcgaac gggtccaggc ggtgaatccc gccgaactgc ccgccgattc gaccgagatc
     3181 gacctgggtg acgtcaccct ggtgccgggt ctgatggaca tggagctcaa cctgctcatc
     3241 ggggggcccg gcggtccgga cggcctgccc agtcccatgc acggagtgca ggacgacccc
     3301 gcgtaccgaa cgctgcgcgg cgcggtgaat gccaaaacca ccctggaggc cgggttcacc
     3361 actgtccgca acctgggact gatggtgaag acgggcggct atctgctcga cgtcgccttg
     3421 caacgcgcga tcgaggcccg gtggcacgtc gggccgcgca tctatcctgc cggtcacgcc
     3481 gtcacgccct acggcggcca cctcgacccg acggtgtttc agcgactcgc gcccggcatc
     3541 atgccgctgt ccgtcgccga gggcatcgcc aacggtgtcg atgatgtgcg cgcctgcgtg
     3601 cgttatcaga ttcgccacgg ggccaagctg atcaaggtct cggcatcggg gggagtgatg
     3661 tcgcacagca ccgcgccggg cgcgcagcag tactccgacg acgagttcgt cgcgatcgcc
     3721 gacgaggcac accgggcggg cgtccgggtc gccgcgcatg cggtggggga cagcgccatt
     3781 cgggcgtgca tccgggcggg catcgactgc atcgagcacg gctttttggc cagcgacgaa
     3841 accatccaga tgatggtcga ccacgacacg tttctcgtct cgacgaccta tctcaccgag
     3901 gcgatggccg tcgaccgcat cgcgcccgaa ctgcgcaaga aggccgaaga ggtcttcccg
     3961 cgggcgcagg cgatgctgcc gaaggcgatc gccgcgggtg tgcgcatcgc atgcggaacc
     4021 gacgcgcctg caattccgca cgggcagaac gcaaaagagc tctgcgcctt ggtggacagg
     4081 ggcatgacgc cgatgcaggc gctgcgggcc gccacgatca ccagcgcgga gctgatcgag
     4141 gccgacgagg agctcggcag gctggcgccg ggatacctcg ccgacatcat cgccgtgccg
     4201 ggtgatccgt cacgcgacat cgccaccacg ctcgatgtgc ggttcgtgat gaaaaacggc
     4261 gtggtctaca agcaatgatt tcgaggctgg cctccaggag tgcagtggat atggaacaga
     4321 ccgacaacat tctctggctg ctcaaacagg cgtggtactt ctcgttgacc accgtgaacg
     4381 acgcggtgag cgaacacggc gtcagcaccg cgcagatcgg catgctccgc caactggcca
     4441 ccgagcccgg actgtccggt gccgatctgg cgcggcggct gctgatcagc ccgcagggcg
     4501 tgcaattggc tctcaaggaa ctcgagcgac gcgggctggt cgagcggaag cgggacccgc
     4561 aacacgggcg gatactgcag gccttcctga ccggccaggg tcgaaaagtc gccgaggcgg
     4621 tggtcagtga cgccatcgca gcgcacgcca aggtctttga tgtgctcaca agcgatgagc
     4681 aggaaacgtt gcgtgagctg ctcgggcgcg tgatcgaaca gggcaccggc cacaagctgc
     4741 acaccgatca catcgggccc gagtagcaag tagttgataa taatgacaag tacttgataa
     4801 tgttggcatt gatgagcaag gacaacggga ccgacgtggc cgtcgccgcg accgaagaga
     4861 ttgtcatcga actggacagt cgcaccacga cggcgaacta ccgcacgggt gacacgctgc
     4921 tgcagacggc gcgttcggcc gggctgcagg cgccgtactc gtgtgaaacc ggctcctgcg
     4981 ggacgtgtat ggctcgcatc accgagggca gcgcgcagat ggtgaacaac gacgcactgg
     5041 aggacgacga ggtggacgag ggatacgtgc tcacctgtca ggcgctaccg accagcccga
     5101 cggtgcgggt gacctatgac ttctagcagt gtgagccgcg ttgcggtggt gaccggcggc
     5161 gcttcgggca tgggcgaggc cacttgccat gagctcggaa ggcgtggcca caaggtcgcc
     5221 gtactagatg tcgacgctca cgccgcacaa cgggtttccg agaatctacg cgccgacggt
     5281 gtgacggcgc ttgccgtcgc cgccgatgtc agtgaccggg ccacagtcga ggaggcgttc
     5341 gccaaggttc gtaccgagct cggccctgtg cagattctgg tcaccagcgc gggtgcggtg
     5401 gacttcgcgc cgttcacgga aatcaccatc gagaactggg agcgtttgat cgcggtgaac
     5461 ttgaccggga cctttcactg ttgccaggtt gcggtgcccg acatggtgga ggccggctgg
     5521 ggccgcatcg tgatgatctc gtcgtcgagc gctcagcggg gctcaccgaa gatggcgcac
     5581 tatgcggcca gcaagggcgc gctgttgtcg ctgacgaaat cgcttgcccg cgaatatggt
     5641 tcggtgggca tcacggtcaa caacgttccc ccatcgggca tcgagacgcc gatgcagcac
     5701 cagggacagg cggcgggtca tttgccctcg aatgagcaga tggctgcggg cattccggtc
     5761 ggccacctcg gcaccggcga cgacatcgcc gccgcggtcg gatttctatg ctccgagcag
     5821 gccggcttca tcaccggcca ggtattgggt gtcaacgggg ggcaggtgct gtgacaagac
     5881 gaggaggttg gcatggcgaa gtggcctaaa ccggcggagg gcagctggac ggagcactac
     5941 ccggaattgg gcaccggacc gatctcgttc agggactcca cctcgccgca gttctacgaa
     6001 cttgagcggg aggctatctt caaacgcgcc tggctcaacg tcgggcgcgt cgaggaggtg
     6061 ccccgcgtcg gcagctactt caccaaggag atcgacgtcg ccaagacgtc ggtcattgtg
     6121 gtgaagggca aggaccaaca gatccgagcc ttctacaaca tctgccgcca ccgtggaaac
     6181 aaattggtgt ggaacgactt tcccaacgaa gaggtcaggg gcgcctgtcg gcagttcacc
     6241 tgcaaatacc acggctggcg ctacgacctc gagggcgcgc tgaccttcgt gcagcagccc
     6301 ggtgagttct tcgacctcga cgacaagcat tacgggttga gcccggtgca ctgcgacgtg
     6361 tggaatgggt tcatcttcat caacctcgac ccggaaccac ggcagagcct gcgtgagttc
     6421 ctcgggccga tgatcaccgc cctggacgac tacccgttcg aaaagatgac cgagcgttac
     6481 gatttcgtcg cccacaacaa cagcaactgg aagatcttcg cagacgcatt ccaggagtac
     6541 taccacgtgc cgtccctgca ttcccagcag gtgccgagcg aggtg
//
LOCUS       DASYLX010000118         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3297174,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000118 DASYLX010000000
VERSION     DASYLX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3297174"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGP27_02830"
     CDS             <1..367
                     /locus_tag="VGP27_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7850284.1"
                     /translation="TATPGPTPHDRNDHQPVLLRRAIEFIETNVIEDIGLGDIAEAVC
                     LTPRAVQYMFRRHLDMTPLQYLRRIRLHHAHAELLASDRRHDTVTAISARWGFMHTSR
                     FAVFYRQTYGQSPHVTLRK"
     gene            652..1119
                     /locus_tag="VGP27_02835"
     CDS             652..1119
                     /locus_tag="VGP27_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7850285.1"
                     /translation="MALMRFDPFREIDRLAEQALAGPRNVRTLPMEAFRRGDQFIVAL
                     DVPGMQPSDIDVTVERNVIEITARRRPLRQEGDELIIDERPQGEFRRQLFLGDNLDPN
                     KLTAACDLGVLTMTIPVSEQSKPRKVEIGNPNEGQQAIQPESKEPQTANASVQ"
     gene            1119..1622
                     /locus_tag="VGP27_02840"
     CDS             1119..1622
                     /locus_tag="VGP27_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEV7850286.1"
                     /translation="MATHSTVQTSAVRPNEPARQVIATFDNYADAERAVDYLSDQRFE
                     VNRVAIVGRDLEYVEQVMGRLNYGGAALRGAGSGALVGLLIGWLFGLFNWVQPLVSAL
                     VLAGYGLVFGAIVGALFGLLVHALQRGRRDFHSVSGLRPKYYDVVADVEVADRALQLL
                     TSDNRRE"
     gene            1626..>2870
                     /gene="dnaK"
                     /locus_tag="VGP27_02845"
     CDS             1626..>2870
                     /gene="dnaK"
                     /locus_tag="VGP27_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV7850287.1"
                     /translation="MAIAVGIDLGTTNSVIAAWQGGEPIVIPNAEGARTTPSVVAFTE
                     SGERLVGQLARRQAIMNPKGTIYSAKRFIGRRFDEVSDEAKAVSFDVVPGPNGEARFD
                     VRGKLYSPEEISALILRKLVDDAGKFLGERVKEAVITVPAYFNDAQRNATKDAGRIAG
                     LDVLRIINEPTAAALAYGMDKKGHETVLVFDLGGGTFDVSLLDVGDGVVEVRATAGDS
                     HLGGDDFDRRLVDYLADEFQRENNIDLRKDPQALQRLFEAAEKAKVELSSVAQTQVNL
                     PFITADANGPKHLTMTINRSKFEELTHDLVERTMGPVQQAMSDAKVTANDIDEVILVG
                     GSTRIPAVQALVRRLTGGKDPNMTVNPDEVVALGAAIQAGVLKGEVSDVLLLDVTPLS
                     LGVETAGGLMTKIIERNTTIPVR"
BASE COUNT          538 a          939 c          904 g          489 t
ORIGIN      
        1 tacggcaacg cccggtccga cgccgcatga tcgcaacgat caccaacccg tgctgctgcg
       61 ccgcgccatc gagttcatcg agaccaatgt catcgaggac attggattgg gcgatattgc
      121 cgaagctgtc tgtctcaccc cacgtgcggt gcaatacatg tttcgccgtc atctggacat
      181 gaccccgttg cagtaccttc gccggatccg attgcaccat gcccacgctg aattgctggc
      241 ctccgaccgc agacacgaca ccgtcaccgc gatctcggcc cgttggggat tcatgcacac
      301 cagtcggttc gcggtcttct accgccagac ctacggccaa agtccgcatg tcacgctgcg
      361 taaatgaccg ggcggcacgc ctgatcccgt cgtcgggacg agggtacgac gtgacccccc
      421 tcgtggtggg tcaacccggc ggggttggga gctttggaac ggtccgtcca ttgagggcgt
      481 ccattcgtgc tgacaggtcc aatctcgatc cacatttccg gctcgttggg agcacgcgcc
      541 cgccgaattg acttgccaga cttgttgttc gggagcactg gcgggccgtg cttacaggcg
      601 atcggctgcc ggagcttccg gtacagcaac ccctgaaacc taggagaagc catggctttg
      661 atgcgattcg atccgtttcg cgaaatcgac cggctggccg aacaggccct ggccggccca
      721 cgcaatgtgc gcactctccc catggaggcg ttccgccgcg gtgaccaatt cattgtcgcc
      781 ctcgatgtac cgggcatgca gccgagcgat atcgacgtca ccgtcgagcg caacgtcatc
      841 gagatcaccg cgcgccgccg cccgctgcga caggaaggcg acgagctgat catcgatgaa
      901 cggccgcaag gcgagttccg tcgccaactg ttcctcggcg acaacctcga cccgaacaag
      961 ctgaccgcgg catgcgacct gggcgtgctg acgatgacca tcccggtttc ggagcagagc
     1021 aagccccgca aggtcgagat cggcaacccc aacgagggac agcaagccat tcagcccgaa
     1081 tccaaggagc cacaaaccgc gaatgcgtcg gtgcagtgat ggctacgcat tcaacggtgc
     1141 aaacctcggc ggtgcggccc aacgaacccg cccgccaggt gatcgcgacc ttcgacaact
     1201 acgccgacgc cgaacgggcg gtcgactatc tgtccgatca acggttcgag gtgaaccggg
     1261 tggccatcgt gggccgcgac ctcgagtacg tcgagcaggt catgggccgg ctgaactacg
     1321 gcggtgcggc gctgcgtggt gccgggtcgg gcgcccttgt cggcctgctg atcggctggc
     1381 tcttcggtct gttcaactgg gtccaaccat tggtctccgc cctggtgctg gccggctatg
     1441 gattggtctt cggcgccatc gtgggcgccc tgttcggact gctcgtacac gctctgcaac
     1501 gcggccggcg cgatttccac tcggtcagcg gattgcggcc caagtactac gacgtcgtcg
     1561 ccgacgtcga ggtagccgac cgtgccctgc agctactcac cagcgacaac cgaagggaat
     1621 agacgatggc aatagcggta ggcatcgacc tcgggacaac caactcggtg atcgccgcct
     1681 ggcagggcgg ggaaccgatc gtcatcccca acgccgaagg ggcgcgcacc acaccctcgg
     1741 tggttgcgtt cacggaaagc ggcgagcgcc tggtcggcca actggccagg cgccaggcga
     1801 tcatgaatcc caagggcacc atctattcgg cgaagcgatt catcggccgc cgcttcgacg
     1861 aggtatcaga tgaagccaag gcggtcagct tcgacgtcgt tcccggtccc aacggcgagg
     1921 cacgtttcga cgtgcgcggc aagctgtatt cgcctgagga gatcagcgcg ctgatcttgc
     1981 gcaaactcgt cgacgacgcc ggcaagttcc tcggtgagcg ggtcaaggaa gcggtgatca
     2041 ccgttccggc ctatttcaac gacgcccagc gcaacgccac caaggatgcc ggccggatcg
     2101 ccggcctcga cgtactgcgc atcatcaacg agcctacggc cgcggcactg gcctacggga
     2161 tggacaagaa ggggcacgag acggtgctcg tcttcgacct gggtggcggc acgttcgacg
     2221 tcagcctgct cgacgtgggt gatggggtgg tcgaggtccg cgccacggcc ggtgattcgc
     2281 acctgggtgg cgacgacttc gaccgccggc tcgtcgacta tctggccgat gagttccagc
     2341 gtgagaacaa catcgatttg cggaaagatc cgcaggcgct gcagcggctg ttcgaggcgg
     2401 ccgagaaggc caaggtcgaa ctgtcgtcgg tcgcgcagac ccaggtcaat ctgccgttca
     2461 tcaccgccga cgccaacggc cccaagcatc tcaccatgac catcaatcgg tcgaagttcg
     2521 aggaactcac gcacgatctg gtggagcgca ccatgggccc ggtccagcag gccatgagcg
     2581 acgccaaggt gacggccaac gacatcgacg aggtgatcct ggtgggcggt tctacccgga
     2641 tcccagcggt ccaggcgttg gtccgccggc tcaccggcgg caaggacccc aacatgaccg
     2701 tgaaccccga cgaggtcgtc gcgctcggcg ccgcgatcca ggccggtgtg ctcaagggcg
     2761 aggtctccga cgtcttgctg ctggacgtca ccccgttgtc gctgggtgtg gagaccgccg
     2821 gcgggctgat gaccaagatc atcgagcgca acaccaccat cccggtacgg
//
LOCUS       DASYLX010000119         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_444479,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000119 DASYLX010000000
VERSION     DASYLX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444479"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1211
                     /gene="hisC"
                     /locus_tag="VGP27_02850"
     CDS             111..1211
                     /gene="hisC"
                     /locus_tag="VGP27_02850"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV7850288.1"
                     /translation="MSQTVDRFVKQGFRSAAPYNAEHHDFAWQHRQLARMMSNECPIP
                     PSEGVRAAVREAFDIGHLYPYSGKDLVAALAQYNSVPPESIVLGNGSTEVLDVLVRLL
                     VGPGDETIIASPTYAFFESQTRINGGVPKAVPLTSSWDLDVEAMLGAITDSTTIIFLC
                     SPNNPTGKGWRRDQLVRLLDTGIPTVVDQAYLECGYAESFAPLVADHSNLVIARTMSK
                     GFGLAALRVGYLIGDPELIDIVQRVRIPFSISLMGIWGCLQALAEPEELKRRREFISS
                     EADRLHAAMQKMEGVESYPSDGNFVLADISASGRSVSAVVDTLLAEDILIRAMGAHGL
                     KGSHVRVTVGTIEHNDRFLKLFEELLLPVGQV"
     gene            1242..2768
                     /locus_tag="VGP27_02855"
     CDS             1242..2768
                     /locus_tag="VGP27_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV7850289.1"
                     /translation="MSPHFLAFDIGTSGVKVVVVDADGALLDSSYRGYGIQMVGDAGV
                     EQDLDLIITSLLDAAHELLRRVAGAVDTSVSIDGISVTAQMFNLVAVDESGKPLIPMI
                     SWLDQRAEGAARALAEIMPPSVQFKRFTAVVSAKDIVPKILWLRDTQPEVYARTAKLL
                     DCKEAVVLHLTGMAVTDHAGASAYRLFAHDTRDWDSDACEAAGIDPDKLPAVRAATDV
                     AGRLLPDIAHRLGLPADIPVYVGVGDVPASQLGSGAVDPGDLHVSLGTAVYFGLMMSE
                     RRVDPCHQLGALAHAEAHQWILWLEIATGGAALAWAVRMLGLGDPGSVDYTQVEQMAR
                     DSAHDMDGLLFAPWLSGERVPVFDDAARASFVGLSLHHGPGHFLRAVMEGVAFQMRWA
                     LQYGLDYGERVKRIRAVGGGSMGSVWTQIIADVLERPLETIAAPQDAGAIGAAACALV
                     GSGAKPDYSFLADRVTVTHTYLPDAGRSREYCARYAQYKRLYDALYPIYHNMRDEARR
                     "
     gene            2765..3424
                     /locus_tag="VGP27_02860"
     CDS             2765..3424
                     /locus_tag="VGP27_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV7850290.1"
                     /translation="MTHRCVVFDLDGVLVMSEHLWEQGWESVSAEHGYLWLSEDTRTC
                     QGKSVPEWARYVSQRTGIDEQAAAEGVIGVVAAAYDRGQVPLIDGALELVQAAAARVP
                     VALASSAPRTIIDRVMMTTPVGEYFRATVSSAEVAAGKPAPDVYQEAVARLGARSAGS
                     YAVEDSSNGIRAAAAAGLTVLGMEHPQYPVDRDASGRAAGVYHSLEDVTAVLVGFLDE
                     R"
     gene            3465..>4093
                     /locus_tag="VGP27_02865"
     CDS             3465..>4093
                     /locus_tag="VGP27_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7850291.1"
                     /translation="MTQVADDLALALEMADAADALTLDRFGALDLRVDTKPDLTPVTD
                     ADESAEELLRSSLAAHRPDDSVFGEEFGGTPAFSGRQWVLDPIDGTKNFVRGVPVWCT
                     LIALLVDGTPVVGVVSAPALGRRWWAGAGEGAFTSFGDAKRRISVSGVADLASASLSF
                     SDLTGWGDRRSRFLDLTDEVWRVRAYGDFWSHCLVAEGAVDIAIEPDLKP"
BASE COUNT          688 a         1336 c         1329 g          740 t
ORIGIN      
        1 gcactgacag cgggagtgtt cttcgttctg gcaggcatct ggtacgcggt gattcttcgt
       61 agtcgtatca atcgtgtgca ggctggcgta ccccagggag gtaccgtccg gtgagccaga
      121 cagtagaccg cttcgtcaag cagggattcc gcagcgctgc accctacaac gccgaacatc
      181 acgacttcgc ttggcagcac cgtcaactcg cgagaatgat gtccaacgaa tgcccgatcc
      241 cgccgtccga gggagtgcgg gcggcggtgc gggaggcctt cgacatcgga catctctacc
      301 cctattcggg caaagatctc gtggcggctc tcgcgcagta caactccgtg ccgccggagt
      361 cgatcgtcct gggcaacggt tcgacggaag tgctcgacgt cctagtgcgg ttgctggtcg
      421 gccccggcga cgagacgatc atcgcgtcgc ccacctatgc gttcttcgag agccagacgc
      481 gtatcaacgg cggcgttccg aaagccgtgc cgttgacgtc ctcgtgggac ctggacgtcg
      541 aggccatgct cggcgccatc accgacagca cgacgatcat cttcctatgc agccccaaca
      601 acccgaccgg aaagggttgg cgcagagacc agctcgtgcg cctgcttgac accggcatcc
      661 ccacggtggt cgaccaggcg taccttgagt gcggctacgc cgaatcattc gcacccctcg
      721 tcgccgacca ttcgaacctc gtcatcgcgc ggaccatgtc gaaggggttc gggctggcgg
      781 cgttgcgcgt cggctacttg atcggcgacc ccgaactcat cgacatcgtc caacgagtcc
      841 ggatcccatt cagcatcagc ttgatgggca tctggggctg tctgcaggca cttgccgaac
      901 cggaagagct gaagcggcgg cgcgagttca tcagctcgga agccgatcga ttgcacgcgg
      961 cgatgcagaa gatggaaggt gtcgaatcgt atccctccga cggcaacttc gtcctggctg
     1021 acatctccgc gagcggtcgc tcggtctcgg ctgtcgtcga cacactcctt gcggaggaca
     1081 tcctgatcag ggccatgggt gcacacgggc tgaaggggtc ccacgtccgg gtgacggtcg
     1141 ggaccatcga gcacaacgac cggtttctca agctgttcga agagcttctc ctgcccgtcg
     1201 gccaggtgta gcttcttgaa ccgaggcgag cggagacttc gatgagcccg cacttcctgg
     1261 ccttcgacat cggcaccagc ggcgtgaagg tcgtggtggt cgacgccgac ggcgcgctgc
     1321 tggacagctc ctaccgcggc tacggcattc agatggtcgg cgacgccggc gtcgagcagg
     1381 atctcgacct gatcatcacg tcgttgctgg atgccgctca cgaattgctg agacgagtag
     1441 caggcgctgt tgacacttcc gtctccatcg atgggatcag cgtcaccgcc cagatgttca
     1501 atctcgtggc ggtcgacgaa tcgggcaagc cgttgattcc gatgatcagc tggctcgatc
     1561 aacgcgctga gggcgctgct cgggcgctgg ccgaaatcat gccgcccagt gtgcagttca
     1621 agcgtttcac cgcggtcgta tccgccaaag acatcgtgcc gaagattctc tggctccgtg
     1681 acactcagcc cgaggtgtat gcccgcacgg cgaagctgtt ggactgcaag gaagctgtcg
     1741 tcctgcatct cacgggcatg gcggtcaccg atcacgccgg ggcatcggcc tatcgacttt
     1801 tcgcccatga cacccgcgac tgggacagcg acgcgtgtga ggcggcgggg atagatcccg
     1861 acaagcttcc cgccgtccgc gccgctactg acgtggccgg tcgactcttg cccgatatcg
     1921 cgcatcgact cggcttgccc gccgacatcc cggtgtacgt cggcgtcggc gacgtgcctg
     1981 cgagccaatt gggctctggc gccgtcgatc cgggcgacct tcacgtcagc ctcggcacgg
     2041 cggtgtactt cggcttgatg atgtccgagc gacgcgtcga tccgtgtcac caacttgggg
     2101 cactggcgca cgccgaggcc caccagtgga tcctctggct cgaaatcgcc accggcggtg
     2161 cggcgctcgc ttgggcggtg cgcatgctgg ggctcggcga tcccggctcg gtggactaca
     2221 cacaggtgga acagatggcg cgggacagtg cacacgatat ggatggcctg ttgttcgcgc
     2281 cctggctcag cggagagcgc gtaccggtat tcgacgacgc tgcccgcgcc tcgttcgtcg
     2341 gcctgagctt gcaccacgga ccaggacact tcctgcgcgc cgtcatggag ggcgtggcct
     2401 ttcagatgcg gtgggcactg cagtacgggc tcgactacgg agagagggtc aagaggatcc
     2461 gcgcggtggg cggcggatcg atgggatcgg tgtggactca gatcatcgcc gacgtcctgg
     2521 agcgtccgct ggaaaccatc gccgcaccgc aggatgccgg ggccatcgga gccgccgcat
     2581 gcgcactggt tgggagcggg gccaaacccg actactcgtt tctcgccgac cgggtgacgg
     2641 ttacccatac ctatctaccc gatgcgggcc gttcgcgcga atactgcgcc cgttatgccc
     2701 agtacaaacg gctctacgac gccctctatc cgatctatca caacatgcga gatgaggctc
     2761 gacggtgaca caccgctgcg tggtgttcga cctcgatggg gtgctcgtga tgtccgagca
     2821 cttgtgggag cagggctggg aatcggtgtc cgccgagcac ggctacctct ggctgtccga
     2881 ggacaccagg acgtgccagg gcaagagcgt ccccgaatgg gcccgctacg tcagtcaacg
     2941 caccggcatc gacgaacaag ccgcggctga aggcgtcatc ggtgtggtcg cggcggcgta
     3001 cgaccgcggc caggttccgc ttatcgacgg agccctcgaa ttggtgcagg ccgccgcggc
     3061 gagagtgccg gtggcgttgg cctcctcggc gccccgcacg atcatcgacc gggtaatgat
     3121 gactacaccc gtcggcgagt acttccgcgc gacggtttcc agcgccgagg tggccgcggg
     3181 aaagcccgcc cccgacgtct accaggaagc cgtcgccagg ttgggtgcac gcagcgcagg
     3241 cagctatgcg gtggaggact cgagcaacgg catccgggcc gccgcagccg ccgggctcac
     3301 agttctgggc atggagcacc cccagtaccc ggtggatcgc gacgcttcag gccgcgccgc
     3361 cggcgtctac cacagcctcg aagacgtcac cgctgtgctg gtgggtttcc tcgacgaacg
     3421 ctagctcgtc atccaccaca cggggccatg gtaggactgc tgccatgacc caggtcgcag
     3481 atgatctcgc cctcgcgttg gagatggccg acgccgcaga cgcactgacg ttggaccggt
     3541 tcggtgcact cgatctgcgt gtcgacacca aaccggacct gacgccggtc accgacgccg
     3601 acgagtcggc cgaagagctg ttgcgctcgt cgctcgccgc gcatcgaccc gacgattcgg
     3661 ttttcggcga agaattcggc ggcacacccg ctttcagtgg taggcagtgg gtgctcgatc
     3721 cgatcgacgg cacgaagaac ttcgtccgcg gagttccggt gtggtgcacg ctgatcgccc
     3781 tgctcgtcga cggcaccccc gtcgtcggcg tcgtcagcgc ccccgcgctc ggacgccggt
     3841 ggtgggcagg cgctggcgaa ggcgcgttca cttccttcgg cgatgcgaag cggcgcatct
     3901 ccgtctccgg agtcgctgac ctcgcctcgg ccagcctgtc cttctccgac ctcaccgggt
     3961 ggggtgaccg ccgatcgcgt ttcctcgacc tcaccgacga ggtctggcgg gtacgcgcct
     4021 acggcgattt ctggtcccac tgcctggtgg ccgagggtgc cgtcgacatc gcgatcgaac
     4081 ctgacctcaa gcc
//
LOCUS       DASYLX010000120         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1760915,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000120 DASYLX010000000
VERSION     DASYLX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760915"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VGP27_02870"
     CDS             complement(<1..167)
                     /locus_tag="VGP27_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEV7850292.1"
                     /translation="MILLPAVDVVDGKAVRLVQGQAGSETDYGSALDAAMTWQRDGAE
                     WIHLVDLDAAFG"
     gene            complement(206..826)
                     /gene="hisH"
                     /locus_tag="VGP27_02875"
     CDS             complement(206..826)
                     /gene="hisH"
                     /locus_tag="VGP27_02875"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV7850293.1"
                     /translation="MTTKSVVVLDYGSGNLRSAQRALERVGAEVEVTSDADAAARADG
                     LVVPGVGAFEACMTGLRDIGGDKIIADRVAVGAPVLGVCVGMQILFARGVEFGVESQG
                     CGQWPGAVVRLDAPVIPHMGWNVVNAAVGSSLFKGLDDTTRFYFVHSYAAQTWEGNPE
                     ARLTWATHSVPFLAAVEDGALAATQFHPEKSGDAGATLLANWVSDL"
     gene            complement(823..1437)
                     /gene="hisB"
                     /locus_tag="VGP27_02880"
     CDS             complement(823..1437)
                     /gene="hisB"
                     /locus_tag="VGP27_02880"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEV7850294.1"
                     /translation="MTTNRRARVERKTKESDIVVELDLDGTGVVEISTGVPFYDHMLT
                     SLGSHASFDLTVEAKGDVEIEGHHTIEDTAIVLGQALREALGDKKGIRRFGDAFIPMD
                     ETLAHAAVDVSGRPYCVHSGEPEHVLHSTIAGSEVPYHTVINRHVFESLAMNARIALH
                     VRTLYGRDPHHITEAQYKAVARALRQAVEFDPRVTGVPSTKGTL"
     gene            complement(1434..2570)
                     /locus_tag="VGP27_02885"
     CDS             complement(1434..2570)
                     /locus_tag="VGP27_02885"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV7850295.1"
                     /translation="MSGLREERTAGPPGQSVTLADLPLRDDLRGKSPYGAPQLAVPVR
                     LNTNENPHPPTQALIDDVTNSVNAVAGELHRYPDRDAVALRTDLAAYLSTQTGVGVVV
                     ENLWAANGSNEILQQLLQAFGGPGRSAIGFVPSYSMHPIISDGTQTDWLVANRGEDFS
                     LDAEVAVHAIKEKCPDIVFVASPNNPSGQSVSLDELRRLLEAMTTGILILDEAYGEFS
                     SQPSAVSLIAEFPTKLVVTRTMSKAFAFAGGRLGYLIAAPAVVDAMLLVRLPYHLSSV
                     TQAAARAALRHADDTLGSVDTLIAERNRVTDALTGMGFDVIPSDANFLMFGKFADAAA
                     AWQRYLDAGILIRDVGIPGYLRATIGLAEENDALLEASARIGAQ"
     gene            complement(2570..>2948)
                     /locus_tag="VGP27_02890"
     CDS             complement(2570..>2948)
                     /locus_tag="VGP27_02890"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV7850296.1"
                     /translation="SAIVLVDDVDAGIKTVNAYAAEHLEIQTADPRDVAGRIRSAGAI
                     FVGAYSPVSLGDYCAGSNHVLPTAGCARHSSGLSVQTFLRGIHVVDYDEAALKDVSGY
                     VITLAEAENLPSHGEAVRRRFEE"
BASE COUNT          481 a          961 c          987 g          519 t
ORIGIN      
        1 ccgaacgcgg cgtcgaggtc caccagatgg atccactccg ccccgtcgcg ctgccaggtc
       61 atcgcggcgt ccagtgcgga gccgtagtcg gtttcgctgc cggcctggcc ctgcacgaga
      121 cgcacggcct tgccgtcgac gacgtcgacg gcaggcaaaa ggatcaattg cttatttcct
      181 ggcacggagg cctaacctag cgtcctcaca ggtccgacac ccaattggca agcaatgtcg
      241 cacccgcgtc gccgctcttc tcgggatgga actgggtggc ggccagtgca ccgtcctcga
      301 cagcggccag gaatggcacg ctgtgtgtgg cccaggtcag tcgggcttcg gggttgccct
      361 cccacgtctg cgcggcatag gagtgcacga agtagaagcg ggtggtgtca tcgagcccct
      421 tgaacagcga actgccgacg gcggcattga caacgttcca acccatgtgc ggaatcaccg
      481 gcgcatccag gcggaccacc gcgccgggcc actggccgca gccctgcgac tcgacgccga
      541 attccacacc gcgcgcgaac aagatctgca tgccgacgca gacgcccagc accggggcgc
      601 ccacggccac gcggtcggcg atgatcttgt cgccgccgat atcgcgcagt ccggtcatgc
      661 aggcctcgaa cgcgccaacg ccaggcacca ccagcccgtc agcgcgcgcg gccgcatcgg
      721 cgtccgaggt cacctcgacc tcggcaccga ccctttccag cgctcgctgc gccgaccgca
      781 gattgcccga gccgtaatcg agtaccacga cggacttcgt tgtcacagag tgcctttcgt
      841 ggatggcacg ccggtcactc tcgggtcgaa ctcgacagcc tgccgcagcg cccgcgcgac
      901 ggccttgtac tgagcctcgg tgatgtggtg cgggtcgcgt ccgtacaggg tccgcacgtg
      961 tagcgcgatg cgggcattca tcgcaagcga ctcgaacacg tggcggttga tcacggtgtg
     1021 gtagggcacc tcggaccccg cgatcgtgct gtgcagcacg tgctccggct cgccgctgtg
     1081 cacgcagtag ggccggcccg acacgtcgac ggcggcgtgc gcgagcgttt cgtccatcgg
     1141 gatgaaggcg tcgccgaatc ggcggatgcc cttcttgtcg ccgagcgcct cacgcagcgc
     1201 ctgtcccagg acgatcgcgg tgtcctcgat cgtgtgatgc ccttcgatct cgacgtcgcc
     1261 cttcgcctcg acggtgaggt cgaagctggc gtgactgccg agcgaggtga gcatgtggtc
     1321 gtagaacggc acaccggtgg agatttcaac gaccccggtg ccgtcgaggt cgagttcgac
     1381 gacgatgtcg gattccttcg tcttgcgttc cacgcgggcg cgacggttcg tggtcactgt
     1441 gctcctattc gtgcgctggc ttccagtagc gcgtcgtttt cctcggccag gccgatggtg
     1501 gcgcgcagat atccggggat acccacgtcg cggatgagga tgcccgcgtc gagatagcgc
     1561 tgccaggcgg ccgcagcgtc ggcgaatttc ccgaacatca agaagtttgc atcgctgggt
     1621 atgacgtcaa agcccatgcc ggtcaacgca tctgtgacgc gattgcgttc ggcgatgagc
     1681 gtgtcgacgc tgccgagggt gtcgtcggcg tgccggagtg cggcgcgcgc ggcggcctgg
     1741 gtgaccgacg acaagtggta cggcagccgc accagcagca tggcgtcgac caccgcgggt
     1801 gcggcgatga ggtatccgag ccttcccccc gcgaacgcga aggccttgct catggtgcgg
     1861 gtcacgacca gcttggtcgg gaattcggcg atgaggctca ccgcgctggg ttgcgacgag
     1921 aactcgccat atgcctcatc gaggatcagg atgccggttg tcatagcctc gaggagccga
     1981 cgcaattcgt cgagcgaaac actctgtccc gacggattgt tcgggctcgc gacgaacacg
     2041 atgtcggggc atttctcctt gatggcgtgc actgcaacct cggcgtcgag gctgaagtcc
     2101 tcgccacggt tggcgacgag ccagtcggtc tgtgtgccgt cagagatgat cgggtgcatc
     2161 gagtaggacg gcacgaaacc tatcgcgctg cggcccgggc cgccgaacgc ctgcaatagc
     2221 tgctgcagga tctcattgga accattggcg gcccagaggt tttcgactac gacgccgacg
     2281 ccggtctgcg ttgacaggta cgccgccagg tcggtgcgca gagcaaccgc gtcgcggtcc
     2341 gggtagcggt gcagttcgcc tgcgaccgcg ttcaccgagt tggtgacgtc atcgatcagc
     2401 gcctgggtcg gcgggtgcgg gttctcgttg gtgttgagcc gcaccggtac tgcgagttgc
     2461 ggcgcgccgt acggcgactt gccgcgcagg tcgtcgcgaa gcggcaggtc tgccagggtt
     2521 accgattgac ccggcggccc tgcagtccgt tcttcgcgca agccgctcat cactcctcga
     2581 acctccggcg caccgcctca ccgtgcgagg gcaggttctc ggcctcggcg agcgtgatga
     2641 catatcccga cacgtctttc agggccgcct cgtcatagtc gacgacgtgg ataccccgca
     2701 gaaacgtctg caccgagagg ccactggagt gccgcgcaca tccggcggtc ggcaacacat
     2761 ggttggagcc tgcgcaatag tcgccgaggc tgaccggcga atatgcaccg acgaagatcg
     2821 cgccggccga ccggatacgg cctgccacgt cacgggggtc ggcggtctgg atctcgaggt
     2881 gctcggcggc gtaggcgttg acggtcttga tgcctgcgtc gacgtcgtcg acgagcacga
     2941 tcgccgat
//
LOCUS       DASYLX010000121         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_225065,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000121 DASYLX010000000
VERSION     DASYLX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225065"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGP27_02895"
     CDS             complement(<1..185)
                     /locus_tag="VGP27_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850297.1"
                     /translation="MPDLRFDDRVAVVTGGGRGLGRAYAMLLGSQGAKVVVNDPGGSL
                     TGDGTDVGPAEEVVREIV"
     gene            complement(186..>1834)
                     /locus_tag="VGP27_02900"
     CDS             complement(186..>1834)
                     /locus_tag="VGP27_02900"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850298.1"
                     /translation="EDTDVVVLGGGIAGLLAGAYLKKAGVEGVRVIEMAGDFGGVWYW
                     NRFPGIQCDNDAYCYIPLLEELDFIPSKKFADGAEIFEHCRNIGKHFGLYDGALFSTQ
                     VRELRWDGSLERWRISTNRGDDIRARFVVMAQGSYNRPKLPGITGIHDFKGHMFHSAR
                     WDYDYTGGDADGGLHKLADKSVALVGTGATGVQLVPHLGRDAKRLYVFQRTPSSVDER
                     HNTPTDPAWAKSLQPGWQEERKRNFHSWSPFVGVVFGEPDLVCDFWTELGRNMTARIA
                     ASPDPASLAIEQIMAIREEEDYKIMERLRRRIGDVVDDPATAELLKPFYRFMCKRPCS
                     SDGYLSAYNRPNVTLVDVAEAKGVERLTENGIVANGTEYEVDCVIFASGFEISTEISR
                     RYAVEAIEGRDGLSLFDYWKDNYKTLHGITSRGFPNQFFTGFIQGGVSANTTAMFEQQ
                     AKHIAYIISEAQARGATTVEPSQEGQDNWVKTIRDLSIDNSAFELSCTPGYYNNEGRG
                     GAEGNGAFLGDFYSPGFYAFDDLLADWRAKGDLDGLVLGR"
BASE COUNT          317 a          579 c          576 g          362 t
ORIGIN      
        1 acgatttcgc ggaccacctc ctcggccggg ccgacatcgg ttccgtcgcc ggtgaggctg
       61 ccgccggggt cgttgacgac gaccttcgcc ccctgcgacc cgagcagcat ggcgtacgcg
      121 cggcccagtc ccctgccgcc gccggtgacg acggcgaccc ggtcgtcgaa cctcaagtca
      181 ggcatctatc ttccgagcac gagaccgtcg aggtcgccct tggctcgcca gtcggccagc
      241 aagtcgtcga acgcatagaa cccgggcgaa tagaagtcgc cgaggaacgc gccgttgcct
      301 tctgcaccgc cgcggccctc attgttgtaa tagcccgggg tgcacgagag ttcgaacgcg
      361 gagttgtcga tcgaaaggtc ccgaatggtc ttcacccagt tgtcttgacc ctcttgactc
      421 ggttcgacgg ttgtcgcgcc tcgggcctgc gcttcggaga tgatgtaagc gatgtgcttg
      481 gcctgctgct cgaacattgc ggtggtgttg gccgaaacac caccctgaat gaagccggtg
      541 aagaactggt tgggaaagcc gcggctggtg atcccgtgca gggtcttgta gttgtccttc
      601 caatagtcga agagtgacag cccgtcgcgg ccttcgatgg cctccaccgc atagcggcgg
      661 ctgatctcgg tggagatctc gaaaccactc gcgaagatca cgcaatcgac ttcgtactcg
      721 gtgccgttgg cgacgatccc gttctccgtc aggcgctcca cgcccttggc ttccgccacg
      781 tcgaccaaag tgacgttggg ccggttatag gcggacagat atccgtcgct cgagcacggc
      841 cgcttgcaca tgaatcggta gaacggcttg agcaattcgg cggtggcggg gtcatcgacg
      901 acgtcaccga tgcggcgtcg caaccgctcc atgatcttgt agtcttcctc ttcccgtatc
      961 gccatgattt gctcgatggc cagcgatgcg ggatcgggac tcgccgcaat ccgtgcggtc
     1021 atgttgcggc ccagttcggt ccagaagtcg cagaccaggt ccggctctcc gaacaccacg
     1081 ccgacaaacg gtgaccatga gtggaagttg cgcttgcgct cttcctgcca gcccggctgc
     1141 agcgatttgg cccaggccgg atcggtcgga gtgttgtgcc gctcgtcgac tgatgacgga
     1201 gttcgttgga acacatagag ccgcttggca tctcggccga gatgcggcac cagctgaacg
     1261 cccgtcgccc cggttccgac gagcgcgaca ctcttatcgg cgagtttgtg cagtccgccg
     1321 tcggcgtcgc cgccggtgta gtcgtagtcc caccgggcgg agtggaacat gtggcccttg
     1381 aagtcatgga tcccggtgat gcccggcagt ttcggccggt tgtaggagcc ctgggccatg
     1441 acaacgaagc gggccctgat gtcatctccg cggttggtgc tgattcgcca ccgctcgagc
     1501 gaaccgtccc agcgcagttc gcgcacctgg gtggagaaca gcgctccgtc gtacagaccg
     1561 aagtgctttc cgatgttgcg gcaatgctcg aagatttcgg cgccgtcggc gaacttcttc
     1621 gacgggatga agtcgagttc ttcgagcagc gggatgtagc agtacgcatc gttgtcgcac
     1681 tggatgccgg gaaaccggtt ccagtaccag acaccgccga aatcgcctgc catctcgatg
     1741 acgcggacac cttcgacgcc ggccttcttg aggtaggccc cggcgagcag gcctgcgata
     1801 ccgccaccaa ggacgacgac atccgtgtcc tcgg
//
LOCUS       DASYLX010000122         4999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_115805,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000122 DASYLX010000000
VERSION     DASYLX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115805"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGP27_02905"
     CDS             complement(<1..681)
                     /locus_tag="VGP27_02905"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207757.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV7850299.1"
                     /translation="MTSVTRPPTIRSPRGTTISCKGWQQEAALRMLMNNLDPEVAEHP
                     EDLVVYGGTGRAARNWPAFDAIVATLQDLEDDETLLVQSGKPVGVFRTHVWSPRVLIA
                     NSNLVGDWANWEKFRELEAAGLMMYGQMTAGSWIYIGTQGILQGTYETFGAVAKKLAA
                     SGRYQHSGGTLAGTITLTAGVGGMGGAQPLAVTMNDGVAICVDCDESRIDRRIAHRYL
                     DLKAGSVDD"
     gene            775..1572
                     /locus_tag="VGP27_02910"
     CDS             775..1572
                     /locus_tag="VGP27_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7850300.1"
                     /translation="MSSTSPAVGRALDVLLYLAGRPGPVSGAAIIRDLDIPRSSTYHL
                     LDVLTERGFVVRLADQRTYGLGVSAFEVGSAYLRHGPLEMWARPVLKQLVAQVHETCH
                     LGILHGSETLYLLREHPTAAHMAVTLTGIGVRMPAHLTASGRAILAHLPAAQVRGLFP
                     STRAFVNRTGEGPTSLPELRRLLQAERRRGYAEEVGLITPGLQSVAACAFDHTGHPCA
                     AFSVTRRQAGSHVPAAELVRAARSAAAQLTSALSGRPPAGWFAAQPA"
     gene            complement(1560..2060)
                     /locus_tag="VGP27_02915"
     CDS             complement(1560..2060)
                     /locus_tag="VGP27_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850301.1"
                     /translation="MRRRDGVVDGVIRMASATAALAALLVIALPSAAAAPAADERGYA
                     NSSARCEAPNSVVVFGSTDASRIAICKTPGGDYEYRGVRLSDGAKLIVPAQAPPDGAF
                     IAENGPISYLVTMKSLVVSQGTTVIREEPMVDFHGSQAPAAAPATPTSTAPLPPPLPA
                     EKGHAG"
     gene            complement(2094..2657)
                     /locus_tag="VGP27_02920"
     CDS             complement(2094..2657)
                     /locus_tag="VGP27_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850302.1"
                     /translation="MSRWEPNSRGRLEEAALDLYTERGFDETTVAEIAERAGLTERTF
                     FRHFADKREVLFGGQDALLQLFVDTIVEAPESMSPLDAVGAALAAAGPVFEDRHKHAR
                     RRQAVIAANPRLQERELIKLSSIASAIADALRRRGVTDSAAQLTAEAGVAVFKVAFDR
                     WINEGRRDQLPQYIRDSIEELRAVSRG"
     gene            2742..3629
                     /locus_tag="VGP27_02925"
     CDS             2742..3629
                     /locus_tag="VGP27_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005116176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850303.1"
                     /translation="MRIFVTGATGFIGSAVVAELIGAGHQVVGLARSNSSAQAAADAG
                     ADVQRGSLDDLDSLRQAAESSDGVIHLAFNHDFNDFANGAELDRRAIETLGGALAGSD
                     RPLVVAGGILWLSPGKTLTEDQPAPPDLPRFSEKAVLEFTDRGVRAAVVRLPPSVHGE
                     GDHGFVPRLIEVAHEKGRSAYVGDGSNRWPAVHRIDAARAFRLAVESAPAGSILQATD
                     DDGVPARAIADTIGKHLDLPVTSVAPEQSYEHFGWIGPLFSVDAPATSVRTRERLGWR
                     PEHAGLLDDLDAGHYFGEA"
     gene            3702..>4999
                     /locus_tag="VGP27_02930"
     CDS             3702..>4999
                     /locus_tag="VGP27_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEV7850304.1"
                     /translation="MSQNVADFVLDRLRQWGVAQVFGYPGDGINGLVAAFGRADNKPR
                     FVQTRHEEMAAFAATGYAKFSGEVGVCMATSGPGAIHLLNGLYDAKLDHVPVVAIVGQ
                     TERSAMGGSYQQEVDLQVLFRDVASDYLVEVNVPQQLPLAIDRAIRTARTRRSPTAVI
                     IPSDLQDEKYSPPAHAFKQVPSSDPGDNIGRITPTDEQVRAAADILNAGEKVAILIGQ
                     GARGAADEVAAVAELTGAGVAKALLGKDVLPDDLPYVTGSIGLLGTRPSYELMRDCDT
                     LLIVGSNFPYSQFLPDYGAARAVQIDIDGSSIGMRYPTEVNIVADAKAALGALIPHLR
                     PKADPSWRSGVERNVAHWWEVLERQCLLTAKPVNPMRVTWELSQRLPADAIVTADSGS
                     STNWFARCVKLRSGMRASVSGTLATMGCAVPYAIGAKFAHP"
BASE COUNT          790 a         1718 c         1686 g          805 t
ORIGIN      
        1 atcgtcgacg ctgcccgcct tgaggtcgag gtagcgatgg gcgatgcgcc gatcgatgcg
       61 gctctcgtcg cagtccacac agatcgcgac gccgtcgttc atggtcaccg cgagcggctg
      121 cgcgccaccc attccgccga cgcccgcagt gagagttatc gtcccggcga gtgttccgcc
      181 gctgtgctga tagcggccgg aggccgccag cttcttggcg acggcgccga aggtctcgta
      241 ggtgccctgc aggattccct gcgtgccgat atagatccag gagcccgcgg tcatctggcc
      301 gtacatcatc aggcccgcgg cctccagctc acgaaacttc tcccagttcg cccagtcgcc
      361 aacgagattg gagtttgcga tcagaacgcg cggtgaccag acgtgcgtcc ggaaaacacc
      421 caccggcttg ccggactgaa ccagcagagt ctcgtcgtcc tcgaggtcct gcagcgtcgc
      481 gacgatcgcg tcgaacgccg gccagttgcg ggctgcgcga ccggtgccgc catagaccac
      541 caggtcttcg gggtgctcgg caacctcggg gtccaggttg ttcatcaaca tgcgcagcgc
      601 ggcttcctgt tgccatccct tgcaggagat ggtcgtgccg cgaggggagc ggatggtggg
      661 cggccgggtt actgaagtca tgagatcagc acaccccatc acctgtcgtc gcatccagac
      721 ctgttcgccg agtaatgtct cgtatccgag acctaagcag gaggcgggcg ggctatgtcg
      781 agcacctcac cggcggtggg acgggcgctc gacgtcctgc tctacctcgc cggacgaccc
      841 ggcccggtct ccggcgcggc gatcatccgc gatctcgaca tcccgcgctc gtcgacgtat
      901 cacctgctcg acgtgctcac cgaacgtggc ttcgtcgtgc gcctggcgga tcagcgcacc
      961 tacggactgg gggtgtcggc gttcgaggtg ggttcggctt atctgcgaca cgggccgctg
     1021 gagatgtggg cccgcccggt gctcaagcaa ctcgtcgccc aggtgcacga gacctgtcac
     1081 ctcggcatct tgcacggctc cgagacgctg tatctgcttc gcgagcatcc gacggcggcg
     1141 cacatggccg tcacgctcac cgggatcggc gtccgcatgc ccgcgcacct gactgccagt
     1201 ggccgcgcga tcctggcgca tctgcccgcc gcccaggtgc gtgggttgtt cccgtcgacc
     1261 cgcgccttcg tcaaccgaac gggagagggg ccgacctcac tgccggagct gcggcgactg
     1321 ttacaggccg agcggcgtcg gggctatgcc gaggaggtcg ggctcatcac gcccgggctg
     1381 caatcggtgg cggcgtgcgc gttcgaccac accggccacc cgtgcgcggc gttcagcgtg
     1441 acccggcgcc aggctgggtc ccatgtgccc gcggcggagt tggtgcgcgc cgcccgatcc
     1501 gcagctgcgc agctgacgtc ggcgctgtcg ggtcgaccac cggccggctg gtttgcggct
     1561 cagccggcgt gacccttctc tgcgggcagt ggcggcggca acggcgccgt cgacgtcggc
     1621 gtggccggtg cggcggcggg cgcctgactg ccgtgaaaat ccaccatcgg ttcctcgcgg
     1681 atcaccgtgg tgccctggct gacgacgagt gacttcatcg tcaccaggta gctgatcggg
     1741 ccgttctcgg cgatgaaggc accgtcaggt ggagcctggg ccgggacgat cagcttcgcg
     1801 ccgtcgctca accgcacacc gcgatattcg tagtcaccac ccggcgtctt gcagatcgcg
     1861 atgcgcgaag cgtccgtgct gccgaagacg acgaccgagt tcggcgcttc acagcgcgcg
     1921 ctcgaattcg cgtagccccg ctcatcggcg gccggcgcgg cggcggccga cggcagcgcg
     1981 atgacgagca acgccgccag cgcggccgtc gccgaagcca tccggatcac accgtccacc
     2041 acaccatcgc gtcggcgcat cgagaagcgt cgacgccggt cacttcggaa tcgttagccc
     2101 ctcgagaccg ctctgagttc ctcgatggag tcgcggatgt actgcggcag ctgatctcgc
     2161 cgcccctcgt tgatccaccg gtcgaaggcg accttgaaaa cggcgacgcc cgcctcggcg
     2221 gtcagttgcg ccgccgagtc ggtgacaccg cggcgacgca gcgcatcggc gatggcggag
     2281 gcgatcgatg agagcttgat cagctcgcgc tcctgcaggc gggggttggc ggcgatgacg
     2341 gcctggcgcc gcctggcgtg cttgtggcgg tcctcgaaca ccggacccgc agccgccaac
     2401 gcggcgccga cggcatcgag cggcgacatc gactcgggag cctcgacgat ggtgtcgacg
     2461 aacagttgca gcagggcgtc ctgaccgccg aacagcactt cgcgcttgtc ggcgaagtgc
     2521 cggaagaagg tccgctcggt gaggcccgcc cgctcggcga tctcggcgac cgtcgtctcg
     2581 tcaaacccgc gttcggtgta taggtcgagc gccgcctcct ccaggcgacc ccgcgagttg
     2641 ggctcccacc ggctcatgta cggatggtac gggaatgaca gcgactgaca tcagcgttac
     2701 cgtgatgaca gcagctgaca tcagcggaag gagacgaaga catgcgcata ttcgtgacag
     2761 gcgcgacggg gttcatcggt tcggcggtgg tggccgaact catcggagcc ggacaccaag
     2821 tggtgggcct cgccagatcc aactcgtcgg cgcaggccgc ggctgacgcg ggcgccgatg
     2881 tgcagcgcgg cagcctcgac gatctggaca gcctgcggca agcggccgaa tcatcggacg
     2941 gtgtcataca tctcgcgttc aatcatgact tcaacgactt cgccaacggc gccgaacttg
     3001 accggcgcgc gatcgaaacg ctcggagggg cgctcgcggg ctccgaccgg ccgctcgtcg
     3061 tcgcgggcgg aatcctctgg ctctcgccgg ggaagaccct caccgaggac cagcccgccc
     3121 cgcccgacct cccgcgattc tccgagaagg ccgtgctgga attcaccgac cgcggggtgc
     3181 gtgccgccgt ggtgcgcctg ccaccgagcg tgcacggcga gggcgatcac ggcttcgtgc
     3241 cccgactcat cgaggtcgcc cacgagaagg gccgatcggc gtacgtcggg gacggatcca
     3301 accggtggcc tgccgtgcac cgcatcgacg cagcgcgagc cttccggttg gcggtggaat
     3361 cggcgcccgc cgggtcgatc ctgcaggcca ccgacgacga cggtgtgcct gcccgcgcga
     3421 tcgccgacac catcgggaag cacttggatc tgccggtcac atcggtggcc ccagaacagt
     3481 catacgagca cttcggttgg atcggcccgc tgttctcagt cgacgccccg gcgaccagcg
     3541 tccgaacgcg tgaacgtctg ggctggcgcc ccgagcatgc cggacttctc gatgacctcg
     3601 acgccggcca ctacttcggg gaggcgtgat gcggcacgtc ggccgcaagt agccggccgc
     3661 aagggcgcat actcgccgtt gccccggggt acgggcacaa catgagccaa aacgttgccg
     3721 acttcgtcct cgaccgcctg cgacagtggg gcgttgcgca ggtgttcggc tatccgggcg
     3781 acggcatcaa cggtttggtc gccgcgttcg gacgggccga caacaagccg cggttcgtgc
     3841 agacccggca cgaggagatg gccgcgttcg ccgccaccgg ctacgcgaag ttctccggcg
     3901 aggtcggcgt gtgcatggcc acgtcgggac cgggcgcgat ccaccttctc aatggtctct
     3961 acgacgccaa gctcgaccac gtgcccgtgg tggccatcgt cgggcagacc gaacgcagcg
     4021 cgatgggcgg cagctatcag caggaggttg acctgcaggt cctgttccgc gacgtcgcca
     4081 gcgactacct cgtcgaggtc aacgttcccc agcagttgcc gctcgcgatc gaccgagcca
     4141 tcaggaccgc ccgcacccgg cgctcgccga cggcggtcat catcccgtcc gacctccagg
     4201 acgagaagta cagcccaccg gcccacgcgt tcaagcaggt gccgtccagc gaccccggtg
     4261 acaacatcgg tcggatcacc ccgaccgacg agcaggtgcg cgcggccgcg gacatcctca
     4321 acgcgggcga gaaggtcgcc attctgatcg gccagggcgc gagaggcgct gccgacgaag
     4381 ttgcggccgt tgcggagctc accggggccg gtgtggcgaa agcattgctg ggcaaggacg
     4441 ttctgcccga cgatctgccc tacgtcaccg gctcgatcgg ccttctgggt accaggccga
     4501 gctatgagct gatgcgcgac tgcgacacgc tgctcatcgt gggttccaac tttccctaca
     4561 gccagttcct gcccgactac ggcgcggccc gcgccgtgca gatcgacatc gacggcagct
     4621 ccatcggcat gcgttatccc accgaggtca acatcgtcgc cgacgcgaag gccgcactcg
     4681 gcgcattgat tccgcacctt cggccgaagg ccgacccgtc gtggcggtcc ggggtggagc
     4741 gcaacgtcgc gcactggtgg gaggttctgg agcggcagtg tctgctgacg gccaagccgg
     4801 tcaacccgat gcgggtgaca tgggaactct cccagcggtt gcctgcggac gcgatcgtga
     4861 ccgcggactc cggttcgtcg accaattggt tcgcccgctg cgtcaagctg aggtccggca
     4921 tgcgggcgtc ggtatcgggc accctggcca ccatgggatg tgcggtgccg tacgcgatcg
     4981 gcgccaagtt cgcccatcc
//
LOCUS       DASYLX010000123         8055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3406798,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000123 DASYLX010000000
VERSION     DASYLX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406798"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..331
                     /locus_tag="VGP27_02935"
     CDS             56..331
                     /locus_tag="VGP27_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7850305.1"
                     /translation="MIHVLRSTYLQSAEVVEQTRPAHLEFLKEEIAAGRLLLAGRQDN
                     GSGGMLITADIAVEEANEMIARDPYTKAGAANYERTSFNGAFRAPGL"
     gene            455..1501
                     /locus_tag="VGP27_02940"
     CDS             455..1501
                     /locus_tag="VGP27_02940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7850306.1"
                     /translation="MTTVSAYAATSATEPLTKTTITRRDVGPHDVAFDIHFAGICHSD
                     IHTVRAEWGGVTYPIVPGHEIAGIVSEVGSEVTKYKVGDRVGVGCFVDSCRECEYCLA
                     GEEQYCDNPGMVGTYNGVGRDGQPTQGGYSGAIVVDEDYVLRIPDSISLEAAAPLLCA
                     GITTYSPLRHWNAGPGTKVAVIGLGGLGHLAVKLAVALGAEVTVLSQTLKKMEDGLRL
                     GASEYYATSDPETFEKLAAQFDLILNTVSANLNMADYLGLLKLDGTLVELGMPENPMA
                     VPAGALVFGRRSIAGSLIGGIAETQEMLDFCAEHGVAPEIEVIAPDYINEAYERVLSS
                     DVRYRFVIDTQSLR"
     gene            complement(1504..1830)
                     /locus_tag="VGP27_02945"
     CDS             complement(1504..1830)
                     /locus_tag="VGP27_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEV7850307.1"
                     /translation="MTTTSLFQSILRWLRAGYPEGVPGPDRVPLLALLRATTLTEDEV
                     KEVVRNITDGNAGALTDGEITRDEIAEFISDVTHHDAGPDDVKRVAARLAAAGWPLAG
                     VTAAQG"
     gene            2095..4746
                     /locus_tag="VGP27_02950"
     CDS             2095..4746
                     /locus_tag="VGP27_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7850308.1"
                     /translation="MVALCLGSGIYGSSLGEHVTQSGFFDDGSESAQASRMGDKHYGR
                     DTSGHIVAIYTAPNGKTVDDPAFRKKILNNLAKAEKDHPDQILRSIGYFKSPDLLRNM
                     ADASKKHAFMSIQLKGDNDDLILKNFKAVQQDLADPGVNIQLAGLLPLANELTGTIAT
                     DQQRAEVLAMPLVVVLLFFVFGGVVAAFLPGIIGGLTIAGALGIMRLTALSVPVHYFA
                     QPVVTLIGLGIAIDYGLFVVSRFREEIAEGYDTETAVRRTVVTAGRTIAFSSVLISAS
                     VGGLLLFPQGFLKSLTYAMIASVTLAALLSITVLPAILGMLGPKVDALSVTTLLKVPF
                     LRDWRFSNRIITWLGAKLEKTKTSAQVENGFWGKLADRVMKRPLLFAVPIILGLIVLI
                     LPLGNLALGGISEKYLPPTNPVRQAQEEFDRTFPGFRVEPITLVIQSTDGRPVTDAEV
                     AQVRSEALGIPGFAEPDNDPANMWGERPYLDGASKDPSVRVIQNGLVNRLDAKAKVVA
                     LRALPKPHDLNLLVGGTPALEQDSVATISAKLPQMILIAVSMTTILMFLAFGSLVLPI
                     KAALMNALTLCSTLGVLTWIFIDGHFSSWLNFTPTPLTAPVIGLIIAVIFGLSTDYEV
                     FLVSRMVEARARGMTTEEAIRTGTASTGRLISAAALVLVVVGGAFAFSDLVMMQYLAI
                     GLITALLLDATVVRMFLVPSVMKLLGNDCWWAPRWMKRLQERIGLGEITLEDERKATE
                     AVPEQLDESLAFGTVVGAPTWQKPVHDPSRPTAAALASSGAASAVALADPPRAEPTLP
                     VPTGGGNGDSPFGEPSGDGDGDGDEDGDAASAEPTDDGATVGAQEESADEVEAEAEAE
                     DSADDDEAVEQPADVDEADEAKGSAEE"
     gene            4955..6562
                     /locus_tag="VGP27_02955"
     CDS             4955..6562
                     /locus_tag="VGP27_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7850309.1"
                     /translation="MTTESAPTQGDLEPAPAGLKKGAIGIVAVLFMAVANAAPITAMT
                     SNTPIAVGFGNGLGAPAGFLFATIILTLFAIGYVAMAKYITTAGAFYGFISHGLGQAW
                     GMASGALATFAYVVFEGSLIGGFAYFANDAVNTIVGVDIPWLWFAIGGILVIAVLCHF
                     HISLTAAILSVTLTCEVLVLLALAFSVIFKGGGPDGFLLNETVLPLNAFQSLPAGAFG
                     TAAAAGSAAIGLFFAFWSWVGFETTAVYGEESRNPKKIVPRATLIAVIALGLFYTFIS
                     AMVIAGNGKKASVESSISSNPPDLLFKLVDANLGGVLLDIYKILLVIGSFACAMAFHN
                     AASRYIFALGREIPSTAVRKTIGAAHPTHGSPYIASAVQSIITLVLVLLFTFFTAVQV
                     PDANGTPVDTPTLVPFVNIYGLLALIGTAAILLVQAICSIAVIWYFWVKKTHKGNVIT
                     TFICPLIGGLAMFYVVWLLWDNRAFAAGYAANSNVFKVGPYLILAIFVIGIVYAFWLR
                     STKPDEYAAIGRTVLEEAHERAEEATT"
     gene            6648..7019
                     /locus_tag="VGP27_02960"
     CDS             6648..7019
                     /locus_tag="VGP27_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850310.1"
                     /translation="MTSKARLSCILVIGVGLSVAGCGGSTTTSESSSQATTTQTTSGG
                     TASAESTSAKVSANTASESEIESALAAAGVSNPEKWAHEVVEYRPYPTDDPKLQKLRD
                     NLAKYNPGEETLEKIVSALTP"
     gene            7016..7513
                     /locus_tag="VGP27_02965"
     CDS             7016..7513
                     /locus_tag="VGP27_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850311.1"
                     /translation="MTADAKAVSPVMITLVRRGLLALTLIGIAATAFELTTEHHWNGL
                     EQLIPWFALTALTVALVLAVMPSRGAQLLARVLALVVLGASVYGVLDHVTANYNSGPL
                     DQRFADTWESLSATQRWWYAFTKQVGPSPPLAPGVLAQTALLLLLATVMRGKRAEPGP
                     APAPV"
     gene            complement(7523..>8055)
                     /locus_tag="VGP27_02970"
     CDS             complement(7523..>8055)
                     /locus_tag="VGP27_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7850312.1"
                     /translation="AYLNDPHKFDIEGQPAGLTYAYGNARFARDQFWPALKDISMYSL
                     RVAENGMREPHWHPVTAEMGYVQHGDARMTIMNPDGTLDSWNMTTGDMYFIPRAYPHH
                     IENIGTDEWHFLIFFDQPFPADIGYRASASAYSREVLAATFNTHIDDLPQFPFTPADP
                     LIVKRLNPVDVHAVEE"
BASE COUNT         1411 a         2596 c         2567 g         1481 t
ORIGIN      
        1 gaacggagca ggctcgagat tcagcgctat gccgtcgccg agtagggttt gtcgggtgat
       61 tcacgtcttg aggtcgacgt atctgcaatc ggccgaggtc gtcgaacaga cccgtccggc
      121 ccacctcgag ttcctgaaag aggagatcgc ggcgggccgc ctccttctcg ccggccgcca
      181 ggacaacggg tccggcggga tgctcatcac cgctgacatc gccgtcgaag aggccaacga
      241 aatgatcgcg cgcgatccct acacgaaggc cggcgcggcg aactacgagc gaacctcgtt
      301 caacggcgcg ttccgagcgc caggacttta ggagatacct cacagcaacg ccggggatta
      361 cgacccggcg gaccagggtt gaactgaaca gccgcattgt cgcggctctg cctgcccgct
      421 gagcttgttc tcgcacgcat tgaagaggga tttcatgacg accgtatctg cttacgccgc
      481 cacatctgca acggaaccgc tgaccaagac aacgatcacc cgccgcgacg tcgggccgca
      541 cgatgtcgcg ttcgacatcc acttcgcggg catctgccac tccgacatcc acaccgtcag
      601 ggccgagtgg ggcggcgtga cctaccccat cgtgcctggc cacgagatcg caggcatcgt
      661 cagcgaggtc ggctcggagg tgaccaagta caaggtcggc gaccgcgtcg gcgtcggctg
      721 tttcgtcgac tcctgccgcg aatgcgagta ctgcctggcg ggcgaggagc agtactgcga
      781 caaccccggg atggtcggca cgtacaacgg cgtcggtcgc gacggtcagc cgacacaggg
      841 cggctacagc ggcgcgatcg tcgtcgacga ggactacgtg ctccgcatcc ccgattcgat
      901 ttcgctggaa gccgccgcac cgttgctgtg cgcgggcatc accacgtact caccgctgcg
      961 gcactggaac gcaggacccg gcacgaaggt cgcggtcatc ggcctcggcg gtctcggaca
     1021 cctggccgtc aagctcgcgg ttgcgctcgg cgccgaagtg acggtcctga gccagacgct
     1081 gaagaaaatg gaggacggcc tgcggctcgg tgctagcgag tactacgcca ccagcgaccc
     1141 cgagacgttc gagaagctgg cggcacagtt cgacctgatc ctgaacacgg tgtcggccaa
     1201 cctgaacatg gccgattacc tcggcctgct caagttggac ggcaccctcg tcgaactcgg
     1261 catgcccgag aatccgatgg ccgttccggc cggggcgctg gtcttcgggc gccggagcat
     1321 cgcgggctcg ctgatcggcg gcatcgccga aacccaggag atgctcgact tctgcgccga
     1381 gcacggtgtg gcgcccgaaa tcgaggtcat cgcaccggat tacatcaacg aggcctacga
     1441 gcgcgtgttg tccagtgacg tgcggtaccg gttcgtcatc gacacgcagt cactgcggta
     1501 gcgctacccc tgcgccgcgg tgactccggc cagcggccaa ccggccgccg ccagcctggc
     1561 cgcgacccgc ttgacgtcgt cggggcccgc gtcgtggtga gtgacgtcgg agatgaactc
     1621 cgcgatctcg tcgcgggtga tctcgccatc ggtcagcgcg cccgcattcc cgtcggtgat
     1681 gttgcgcacg acctctttga cctcgtcttc ggtcagggtg gttgcacgca gcagcgcgag
     1741 cagagggacc cgatcgggcc ctgggacgcc ttcggggtat ccggcgcgca gccaccgcag
     1801 gatcgactga aacagcgacg tagtggtcac gccgctaagt gtcaggcctg cgaggtagcg
     1861 acgctacgtc gccttctcac agtttcggat gcattcacgg gccgttcact cgaccatttg
     1921 cttgttccgc accgcatcga tggccacctg gggctgccct gcttgtaatc tcagcgatgc
     1981 aactgagtga cgaagtcaat acgccggcca cggagccacg cgagttaggg gctaatcgtt
     2041 ttcgcctggt ggggtcgaac ggtatatcgc taccgattcg ccgtggtggc agtcatggtg
     2101 gcgctgtgcc tcggtagtgg catctacggc agcagcctcg gcgagcacgt cacgcagagt
     2161 ggcttcttcg acgacggcag cgaatcggca caggcgtcgc ggatgggtga caagcactat
     2221 ggcagggaca cctctggcca tatcgtcgcg atctacaccg cgcccaacgg caagaccgtc
     2281 gacgacccgg ccttccgcaa gaagatcctg aacaacctcg caaaggcgga aaaggaccac
     2341 ccggaccaga ttctgcggtc catcggctac ttcaaatcgc ctgatctgct gcgcaacatg
     2401 gccgacgcgt cgaagaagca cgcgttcatg tcgatccagc tcaagggcga caacgacgac
     2461 ctcattctca agaacttcaa ggccgtacag caggacctgg ccgaccccgg tgtcaacatc
     2521 cagttggccg ggttgttgcc gctggccaac gaactgaccg ggaccatcgc cacagaccag
     2581 cagcgggcag aggtgctcgc catgccgttg gtggtggttc tgctgttctt cgtgttcggc
     2641 ggtgtggtcg cggcatttct gcccgggatc atcggcggcc tgaccatcgc gggcgccctc
     2701 ggcatcatgc ggttgacggc gctgtccgtg ccggtgcact acttcgccca gccggtggtg
     2761 acgctgatcg gcctcggtat cgccatcgac tacggactgt tcgtggtcag tcgcttccgg
     2821 gaggagatcg ccgagggcta cgacaccgaa accgcggtcc gccgcaccgt ggtgacggcc
     2881 gggcgcacca tcgcgttctc gtcggtgctg atctcggcat ccgtcggcgg cctgctgctg
     2941 tttccgcagg gcttcctcaa atccctcacc tacgcgatga tcgcctcggt cacgctggcc
     3001 gcgctcctgt cgattaccgt gctgccggca atcctgggga tgctgggtcc gaaggtcgac
     3061 gccctgagcg tcaccacgct gctgaaggtg cctttcctgc gggactggcg attcagcaac
     3121 cggatcatca cctggttggg tgcaaagctg gagaagacca agaccagcgc gcaggtcgag
     3181 aacggcttct ggggaaagct ggccgaccgc gtgatgaaac gtccgttgct gttcgcggtc
     3241 ccgatcatcc tcggtctgat cgtgctgata cttccgctgg gcaatctggc ccttggcggc
     3301 atcagcgaga aatatctgcc gccgacgaac ccggtgcgcc aggcgcagga ggagttcgac
     3361 cggacgttcc ccggtttccg agtcgagccc atcacgctgg tcatccagag caccgacggc
     3421 cgaccggtca ccgacgccga ggtcgcacag gtccgcagcg aagcgctcgg cattccggga
     3481 ttcgctgaac cggacaatga tccggccaac atgtgggggg agcggccgta ccttgacggc
     3541 gcctcgaaag atccgtcggt tcgggtcatc cagaacggcc tggtcaaccg tctggacgcc
     3601 aaggcgaagg tcgttgctct gagggcgctt ccgaagccgc atgatctgaa cttgttggtg
     3661 ggcggcacac ccgccctcga acaggacagc gtcgcaacca tttccgcgaa gctaccgcag
     3721 atgattctga ttgcggtctc catgaccacg atcctgatgt ttctggcgtt cggatcgctg
     3781 gtgttgccga tcaaggccgc gctgatgaac gccttgaccc tgtgctcgac gctgggcgtg
     3841 ctgacgtgga tattcatcga tggccacttc tccagttggc tcaacttcac gccgactccg
     3901 ttgacagctc cggtaatcgg gctgatcatc gccgtgatct tcggcctgtc aacggattac
     3961 gaggtcttcc tggtatcccg catggttgag gcccgtgcgc gcggaatgac caccgaggag
     4021 gccatccgca ccggcaccgc cagcaccggt cgtctgatca gcgccgcggc actggttctt
     4081 gtcgtggtcg gcggcgcgtt cgccttctcg gatctggtga tgatgcagta cctggcgatc
     4141 ggcctcatca ccgcgctgct gctcgacgcg accgttgtcc ggatgttcct ggtgccgtcg
     4201 gtcatgaaac tgctcggcaa cgactgctgg tgggcaccgc ggtggatgaa acggctgcag
     4261 gagcggatcg gcctcggcga gatcacgctc gaggacgagc gcaaggcgac cgaggcggtc
     4321 cccgagcagt tggatgaatc gctcgccttc ggaacggtcg tcggcgcgcc gacgtggcag
     4381 aagccggtgc acgacccgtc gcgtccgacc gcggcggcac tggcttcctc gggggctgcc
     4441 tctgcggtcg ccctggccga cccgccgcgg gcggagccga ccttgccggt gccgaccggt
     4501 ggcggcaacg gcgactcgcc gtttggagaa ccttcgggcg acggcgacgg cgacggcgac
     4561 gaggacggcg acgccgcatc ggccgagccg accgacgacg gcgcgaccgt cggtgcacaa
     4621 gaggaatccg cagacgaagt agaggccgag gccgaggccg aagactcggc cgacgacgac
     4681 gaggccgttg aacagcccgc cgacgtcgac gaagccgacg aggccaaggg cagcgccgaa
     4741 gaatagcgtc agactaggca ctcgctatcg ccgaaactgc gcacgaatcg cctcggacca
     4801 tcgaatcgcg atctccctgc agtctcgcgg acagggatgt gctgagaatt ttcttcccat
     4861 tcgagttgtc gaaagagccc agcagtgcca cgctcactgc gggaactgcg acgtcgcggc
     4921 tcccttgcac tgcgatccga acgggagagc cgcgatgacg actgaatccg cacccacgca
     4981 gggagacctc gaaccagcgc ctgccggcct gaagaagggc gcgatcggca tcgtcgccgt
     5041 cctcttcatg gccgtcgcca acgccgcgcc gatcacggcg atgacgagca acacccctat
     5101 agcggtgggc ttcggaaacg gtcttggtgc cccggcagga tttctcttcg cgacgatcat
     5161 cctgacgctc ttcgcgatcg gttacgtcgc gatggccaag tacatcacga cggccggcgc
     5221 cttttacggc ttcatctctc acggcctcgg tcaggcgtgg ggcatggcca gtggcgccct
     5281 tgcgacgttc gcctacgtcg tcttcgaggg ctcgctgatc ggcggcttcg cgtacttcgc
     5341 caacgatgcc gtgaacacca ttgtgggagt ggatattccg tggttgtggt tcgccatcgg
     5401 cgggatcctg gtcatcgcgg tgctgtgtca cttccacatc agcctgacgg cggcgatcct
     5461 gagcgtgacg ctgacgtgcg aggtgctcgt cctgctcgcg ctcgcgttct cggtgatctt
     5521 caaaggcggc ggacccgacg gcttcctgct caacgagacg gtgctgccgc tgaacgcgtt
     5581 ccagagcctg cctgcaggag cgttcggtac agcagcagcg gccggttcgg cagccatcgg
     5641 cctgttcttc gcgttctggt cgtgggtcgg cttcgaaacc actgcggtct acggcgagga
     5701 gtcccgcaac ccgaagaaga tcgtccctcg ggccacgctc atcgcggtga tcgctctggg
     5761 cctgttctac acgttcatct cggcaatggt gatagcgggc aacggcaaga aggcgtccgt
     5821 cgagtcctcg atcagctcga acccgcccga tctgctgttc aaactggtgg acgccaacct
     5881 cggcggggtg ttgctggaca tttacaagat cctgctcgtc atcggttcat tcgcatgtgc
     5941 catggcgttt cacaatgccg cctctcgtta catcttcgcg ctcggccgcg aaatcccttc
     6001 taccgcagtg cggaagacca tcggtgcggc ccatccgacg cacggctccc catatatcgc
     6061 gtcggcggtg caaagcatca tcacgctggt gctggtgctg ctgttcacct tcttcaccgc
     6121 ggtacaggtg cccgatgcaa acggcacccc tgtcgacacc ccgacactcg tgcccttcgt
     6181 caacatctat ggtctgctgg ccctgatcgg cactgcggcg atcctgctcg tgcaggcgat
     6241 ctgctcgata gcggtgatct ggtacttctg ggtcaagaag acccacaagg gcaacgtgat
     6301 caccacgttc atctgtccgc tgatcggcgg acttgcgatg ttctacgtcg tctggctgct
     6361 gtgggacaac cgtgccttcg cagcgggtta cgcggcgaac tcgaatgtct tcaaggtcgg
     6421 cccgtatctc atcttggcga tattcgtcat cggcatcgtg tacgccttct ggctgcgctc
     6481 gaccaagccc gacgaatacg ccgccatcgg ccgcaccgta ctggaggagg cacacgagcg
     6541 ggccgaggag gcaacgacct aagcgcctgg gtccgtctgg gccgaagtcg gcctcaccaa
     6601 gccgcttccg tgctttgatt gaccgatccg gtcgagaaag gcgcgaaatg acttctaagg
     6661 caaggctttc ctgcattctg gtcatcggtg tcgggttgtc cgtcgccggc tgcggagggt
     6721 cgaccacgac aagtgaaagc agcagccagg cgaccacaac gcagacgaca tccggcggaa
     6781 cggcctcggc ggaatccacg agtgccaagg tgtcggccaa taccgcctcc gagagtgaga
     6841 tcgagtcagc gctcgcggcc gcaggcgtga gcaatccgga gaagtgggct cacgaggtcg
     6901 tcgagtatcg cccctacccg acggacgatc cgaaactgca gaagctgcgg gacaacctgg
     6961 ccaagtacaa cccgggcgag gagaccctcg aaaagatcgt ctcagctttg acgccgtgac
     7021 ggcagacgcc aaagcggtct cgccggtcat gatcacgctc gttcggcgcg gcctgcttgc
     7081 actcaccctg atcgggatag cggccacagc gttcgaattg accaccgaac atcattggaa
     7141 cgggcttgaa cagctgattc cgtggttcgc gctgacggcg ttgacggtcg cgttggtgct
     7201 ggccgtaatg cccagccgcg gagcgcaact actggccaga gtgctcgcgc tggtggtgct
     7261 cggcgcatcg gtctacgggg tgttggacca cgtcaccgcg aactacaact ccggccccct
     7321 cgaccagcgc ttcgccgata cctgggagtc gctgtctgcg acccagcgct ggtggtacgc
     7381 cttcaccaag caggtcggcc cctccccgcc tctggcgccg ggcgtgctcg cgcagaccgc
     7441 tctgttgcta ctgctggcca ccgtcatgcg cggcaagcgc gccgaaccgg ggcccgcccc
     7501 ggccccggtt taactcgtct cgtcactctt cgacggcgtg tacgtcaacc ggattgagcc
     7561 gcttcacgat cagcggatct gccggcgtga acgggaactg cgggaggtcg tcgatgtggg
     7621 tgttgaacgt cgccgcgagc acctcgcgag agtaggcact cgccgaggcg cggtacccga
     7681 tgtcggcggg gaacggctgg tcgaagaaga tcaggaagtg ccactcgtcg gtgccgatgt
     7741 tctcgatgtg gtgcgggtag gcccggggga tgaagtacat gtcccccgtc gtcatgttcc
     7801 agctgtccag cgtcccatcg ggattcatga tcgtcatacg cgcatctccg tgttgcacgt
     7861 agcccatctc ggcggtcacg ggatgccaat gtggttcgcg cataccgttt tcggcgacgc
     7921 gcagcgagta catcgaaata tccttcagcg ccggccagaa ctgatcgcgc gcgaagcgcg
     7981 cgttgccgta tgcgtaggtc agtcccgcgg gctggccttc gatgtcgaac ttgtgcggat
     8041 cgttcaagta cgccg
//
LOCUS       DASYLX010000124         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3736230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000124 DASYLX010000000
VERSION     DASYLX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3736230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="sufB"
                     /locus_tag="VGP27_02975"
     CDS             complement(<1..914)
                     /gene="sufB"
                     /locus_tag="VGP27_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV7850313.1"
                     /translation="MTTTPEALTQEQTIDSLGRYGYGWADSDVAGASAQRGLSEAVVR
                     DISNKKSEPEWMLDVRLKALRVFDKKPMPNWGSNLEGIHFDNIKYFVRSSEKQAATWD
                     DLPADIKNTYDKLGIPEAEKQRLVSGVAAQYESEVVYHSIREDLEKQGVIFLDTDTAL
                     KEHPELFKQYFGTVIPAGDNKFSALNTAVWSGGSFIYVPKGVHVDIPLQAYFRINTEN
                     MGQFERTLIIVDEDAYVHYVEGCTAPIYKSDSLHSAVVEIVVKPGGRCRYTTIQNWSN
                     NVYNLVTKRARAEAGATMEWVDGNIGSKV"
     gene            complement(911..1729)
                     /locus_tag="VGP27_02980"
     CDS             complement(911..1729)
                     /locus_tag="VGP27_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7850314.1"
                     /translation="MKISPESGGGAATAATSGGGAATAATSGGGAATPATSGGDRHTR
                     GAIVQLLLESGPITAGEIGDRLGISAAGVRRHLDALLDAGEAQSSAAASWQHSGRGRP
                     ARRYRLTAAGRAKMGHAYDDLAVAAMRQLREIGGDEAVQTFARRRIDTILSGVTEGLN
                     DVAPTVDRVAAALTKAGYAATATQVSGPLHGIELCQHHCPVSHAAEEFPELCEAERAA
                     FAEVLGTHVQRLATIVNGDCACTTHVPLNSDQQSSPRRPAHGAEVPTSTQGASI"
     gene            1927..>2167
                     /locus_tag="VGP27_02985"
     CDS             1927..>2167
                     /locus_tag="VGP27_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV7850315.1"
                     /translation="MSEQNRRLVLAQRPSGLVDDDTTRMETGQLPDLEDGEALVKVRY
                     LSIDPTIRTWMDDVPSYLPPIQIGEVVRSGGVGEVI"
BASE COUNT          357 a          700 c          702 g          408 t
ORIGIN      
        1 accttcgagc cgatgttgcc gtcgacccac tccatggtgg cgcccgcctc tgcgcgggct
       61 cgctttgtga ccaggttgta gacgttgttc gaccagttct ggatcgtcgt gtaacggcaa
      121 cggccgcctg gcttcacgac gatctcgacg accgccgagt gcagcgaatc gctcttgtag
      181 atcggcgcgg tgcagccttc gacgtaatgc acgtaggcat cctcgtcgac gatgatcagc
      241 gtccgctcga actgacccat gttctcggtg ttgatcctga agtaggcctg cagcggaatg
      301 tcgacgtgaa cgccctttgg cacgtagatg aacgaaccgc ccgaccacac agcggtgttc
      361 aacgcggaga acttgttgtc gccggccggg atcacggtgc cgaagtactg cttgaacagc
      421 tccggatgct ccttgagggc ggtgtcggtg tcgaggaaga tgacgccctg cttctccaga
      481 tcttcccgga tcgagtgata cacgacctcg gactcgtact gcgcggcgac gccggacacc
      541 agccgctgct tctccgcctc ggggatgccg agcttgtcat aggtgttctt gatgtcggcg
      601 ggaaggtcat cccacgtcgc ggcctgcttc tcgctcgagc gcacgaaata cttgatgttg
      661 tcgaagtgaa tgccctcgag gttgctgccc caattgggca tgggcttctt gtcgaagacc
      721 cgcagcgcct tgagccggac gtcgagcatc cactcgggct cgctcttctt gttcgagatg
      781 tcgcgcacaa cggcctctga cagcccgcgt tgggcgctcg cgcccgcaac gtccgagtcg
      841 gcccagccat agccgtagcg acccagcgaa tcgatggtct gttcctgggt caaggcctcg
      901 ggcgtggtcg tcatatcgac gctccttgcg tgcttgtggg gacttcggcg ccgtgggctg
      961 ggcgccgggg ggatgactgc tggtctgagt tgagcggcac gtgcgtggtg caggcgcagt
     1021 cgccgttgac gattgtggcc agccgctgca catgcgtacc aagcacctcg gcgaacgctg
     1081 cccgttcggc ctcgcacaac tccgggaact cctcggcggc gtgcgacacg ggacagtggt
     1141 gctggcagag ctctattccg tggaggggtc cgctcacctg ggtggcggta gcggcgtaac
     1201 ccgccttggt caatgcagct gcgacgcgat cgacagtagg tgcaacgtca ttcaacccct
     1261 cggttactcc cgaaaggatc gtgtcgatac gacgccgcgc gaaggtctgg acggcttcgt
     1321 cgccgccgat ttcgcggagc tgacgcatgg ccgcgaccgc gaggtcgtcg taggcgtggc
     1381 ccatcttggc gcggcctgcg gcggtgagcc ggtagcggcg ggcgggcctg ccgcggccgc
     1441 tgtgttgcca ggacgcggcc gcactggact gcgcttcgcc ggcgtccagc agcgcgtcga
     1501 ggtgcctgcg cacccctgcc gcagagatgc cgagccggtc gccgatctcg cctgcagtga
     1561 tcgggccgga ctccagcagc agctggacga tcgcaccgcg tgtgtggcga tcaccgccgg
     1621 atgtcgccgg cgtagcggct cctccaccgg atgtcgccgc cgtagcagct ccaccaccgg
     1681 atgtcgccgc cgtagcagct ccaccgccgg actccggact tattttcaca acaccattgt
     1741 gacggaattc cgaaagccgg taaagcaagg taacccttat gtggtcaacc tcgcacgaat
     1801 cctcgcgaga cggaatccgc gcaggcccct actcgtcgca cttacgctgc tggaactccg
     1861 tttcgcggga gaattggccg agtgatgcag gccagcgagc gttagcgccg cacctacgat
     1921 caccgtatga gcgagcagaa ccggcgactg gtcctggccc aacgcccctc tggcctggtg
     1981 gatgatgaca cgacccgcat ggaaaccggg cagctacccg acctcgagga cggcgaggcc
     2041 ctggtcaagg tccgctacct ctcgatagat ccgacgatcc gcacctggat ggacgacgtg
     2101 ccgagctatc tgccgccgat ccagatcggc gaagtcgtcc gcagcggcgg cgtcggcgag
     2161 gtcatcg
//
LOCUS       DASYLX010000125         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2310148,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000125 DASYLX010000000
VERSION     DASYLX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310148"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VGP27_02990"
     CDS             complement(<1..261)
                     /locus_tag="VGP27_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEV7850316.1"
                     /translation="MLLAALRDMQWRKRRFVIAILSTAIIFGMTLVLTGLANGFRAEA
                     LRTVDALGVDTFLVKDGASGPFVGATPFAPVDLPRIASSPGVQ"
     gene            569..724
                     /locus_tag="VGP27_02995"
     CDS             569..724
                     /locus_tag="VGP27_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850317.1"
                     /translation="MTAVQDWLKIRPLDPRTHVKAFICALRGVNAEDAEDVTAEPALI
                     QPGVERC"
     gene            complement(729..2456)
                     /locus_tag="VGP27_03000"
     CDS             complement(729..2456)
                     /locus_tag="VGP27_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850318.1"
                     /translation="MTAAAPVHSSRSRRIAISAGALAVLLGALDTYVVVTIIGDIMRD
                     VGIAVNQIQRVTPIVTGYLLGYIAAMPLLGRASDRFGRKMLLQVSLAGFAVGSIVTAL
                     STDLTMLVIGRVIQGAASGALLPVTLALAADLWAARNRAGVLGGIGAAQELGSVLGPM
                     YGITMVWLFHHWQSVFWVNVPLALIAMAMIHFSLPKRLKSEHPEKVDVIGGVLLAITL
                     GLIVVGMYNPAPDGKQVLPSYGPPLLIGALVAGIAFFIWERFAKTRLIEPAGVHFRPF
                     LAALGASLTAGAALMVTLVNVELFGQGVLGQDQNHAAFLLLRFLIALPIGALLGGWIA
                     TRIGDRAVACVGLLIAAGGYYLIAGWPVDLLSARHDLGFVTLPMLDTDLAIAGLGLGL
                     VIGPLTSATLRVVPSAQHGIASAAVVVARMVGMLVGIAALSAWGLYRFNQILQSLPAT
                     KADNLAARLTAEALKYRTAFAMQYGEMFSITAIVCVVGAVLALFISGRSEHADEPADA
                     APGVDAADPATEMIDAPTKANGGPQRVPGFQNPTHTVARGDSEQTARMQRRPSGPPGP
                     PSDRHRPPR"
     gene            complement(2456..3172)
                     /locus_tag="VGP27_03005"
     CDS             complement(2456..3172)
                     /locus_tag="VGP27_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV7850319.1"
                     /translation="MQTRPKNAVRSLWPIFTALFAAIVLIAGCSSSSEKSNESLPDAA
                     TLLKESTASTRALKSIHLDLTVEGQINNFPIKTLSGDLTQAPAVAGQGNTKLTFQGSD
                     VDAKFVVIDSNLYVALSGDSYIDMGPAADVYDISAILSPDKGLANVLANFSDPKSEST
                     ETVNGVETVKVTGQVSADAVNAIAPPIKASGPVPGTAWIEKDGDHKLVQAKLAPSDGT
                     SVQMTLSDWDKPVTVTKPAV"
     gene            3213..3809
                     /locus_tag="VGP27_03010"
     CDS             3213..3809
                     /locus_tag="VGP27_03010"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV7850320.1"
                     /translation="MFTGIVEELGEVVGKEDLADAARFVIRGPVVTADARHGDSISVN
                     GVCLTVVEVQAGGEFSADVMKETLDRSSLGGVGVGSNVNLERAAAVNSRLGGHIVSGH
                     VDGTGHVIVRTPSEHWEVVRIALPTALSRYVVEKGSITVDGVSLTVSGLGHDWFEVSL
                     IPTTLQLTTLGRAAVGTSVNLEVDVIAKYVERLMLDRD"
     gene            3890..4150
                     /locus_tag="VGP27_03015"
     CDS             3890..4150
                     /locus_tag="VGP27_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850321.1"
                     /translation="MKKLAAALLTATAAAVAPIAIAPVAQADICAGAHGRHVGVGGCT
                     NIAGDVATGVAVASAADPYVPGEVPCYTVEGVPYYTPPGDPC"
     gene            4251..>5038
                     /gene="ribB"
                     /locus_tag="VGP27_03020"
     CDS             4251..>5038
                     /gene="ribB"
                     /locus_tag="VGP27_03020"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEV7850322.1"
                     /translation="MTRLDSVDRAVADIAEGKAVVVIDDEDRENEGDLIFAAEKATPE
                     LVAFMVRYTSGYLCVPLDGAICDKLGLLPMYAVNQDKHGTAYTVTVDAKEGVGTGISA
                     SDRATTMRLLANPNSVAEEFTRPGHVVPLRAKDGGVLRRPGHTEAAVDLARLAGLQPA
                     GAICEIVSQKDEGAMAQTDELRVFADEHDLALISIADLIEWRRKHEKHIERVAEARIP
                     TRHGEFRAVGYTSIYEDVEHVALVRGDISGPESDGHDVLVRVHS"
BASE COUNT          902 a         1605 c         1714 g          817 t
ORIGIN      
        1 ctggaccccg ggcgaggagg cgatccgggg cagatcgacc ggtgcgaacg gcgtcgcccc
       61 gacgaagggc cccgacgcgc cgtccttcac caagaaggta tcgacgccga gcgcgtcgac
      121 ggtccgcagt gcctcggccc gaaacccgtt cgccaacccg gtcagtacca gcgtcatccc
      181 gaagatgatg gcggtggaca ggatggcgat gacgaaacgc cgttttcgcc actgcatgtc
      241 gcgcagcgcg gcgagcaaca tggaagcgac gttaccgagg cccgcgcgtt cgcgggccag
      301 gttggggtgc gtcgggttca gcggtctcag ccgacacatg acggatcact ccgccgtcgg
      361 tcggcaacgc tggccgggcc atggttcgcg gcggcgtttg ccggatgtga acgaatgtga
      421 cgaaaacgac tgtgaaatac ccgttgtcgt ggcgcacgat ttgttgggca gggccggtag
      481 actgacgcca gagccccggc atcaggcctg gtacatcgcg ctttcaccga cgagctgcgc
      541 atggtgagca cgaacgaagg aaacgagcat gactgcagtg caggactggc tcaaaattcg
      601 cccgctggat cccagaaccc atgtcaaggc gttcatctgc gcactacgcg gcgttaacgc
      661 cgaggatgcc gaggacgtca ccgccgaacc ggcgttgatc cagcccggcg tcgagcgctg
      721 ctgacgccct agcgaggtgg ccggtgccgg tccgacggcg gcccaggcgg tccagatggc
      781 cggcgttgca tccgcgccgt ctgttccgaa tcaccccgcg caaccgtgtg cgtcggattc
      841 tgaaatcccg gcacacgttg cgggccgcca tttgcctttg tcggcgcgtc gatcatctcg
      901 gtggcgggat cagcagcgtc gacaccgggt gcggcgtccg ccggttcgtc ggcatgttcg
      961 gaacggccgg agatgaacag cgccagcacc gcgccgacga cgcagacgat cgcggtgatg
     1021 ctgaacatct cgccgtactg catggcgaaa gcggttctgt acttcaacgc ttccgcggtc
     1081 agtcgggcgg ccaggttgtc ggccttcgtg gcgggcaacg actgcaggat ttggttgaac
     1141 cggtagagac cccatgcgct gagcgccgcg atacccacca gcatgccgac catccgggcc
     1201 accaccaccg ccgcggaggc gatgccgtgc tgggccgacg gcacgactcg cagtgtcgcc
     1261 gaggtcagtg ggccgatcac caggccgaga ccgagcccgg cgatcgccag gtcggtgtcg
     1321 agcataggca gcgtgacgaa gccgaggtcg tggcgggccg acaacaggtc gaccggccac
     1381 ccggcgatga ggtaatagcc gcccgcggcg atcagcaggc cgacgcacgc caccgcgcgg
     1441 tcgccgatcc tggtggcgat ccagccgccc agcagcgcgc cgatgggcag cgcgatcaga
     1501 aagcgcagaa gcaggaacgc cgcgtggttc tggtcctggc ccagcacacc ctgaccgaac
     1561 agctcgacgt tgacgagcgt caccatcagt gcggcgcccg cggtcagcga cgcgccgaga
     1621 gcggcaagga agggccggaa gtgcacaccc gccggctcga tcagccgggt cttggcgaag
     1681 cgctcccata tgaagaacgc aatgccggcg accaaggccc cgatcagcag cggcggaccg
     1741 taggacggca gcacctgctt gccgtccggc gccgggttgt acatgccgac aacgatcagc
     1801 ccgagcgtga tcgccagcag cacaccgccg atgacatcga ctttctccgg gtgctcgctc
     1861 ttgagccgct tgggcaggct gaagtggatc atcgccatcg cgatcagcgc cagcggcacg
     1921 ttgacccaga acaccgactg ccagtggtgg aacagccaaa ccatggtgat gccgtacatc
     1981 gggccgagca cgctgcccag ctcctgcgcg gcgccgatcc cgccgagcac gccggcgcgg
     2041 ttgcgcgccg cccacagatc ggcggccagc gccagtgtca ccggtagcag cgcaccgctg
     2101 gcggcgcctt gtatgacgcg cccgatgacg agcatcgtca ggtcggtcga caacgccgtg
     2161 acgatcgaac ccaccgcgaa acccgcgagg ctgacctgca aaagcatttt gcggccgaac
     2221 cgatccgacg cccggcccag caggggcatc gccgcgatgt agccgagcag gtagccggtg
     2281 acgatcggtg tgacgcgctg gatctggttc accgcgatcc cgacgtctct catgatgtcg
     2341 ccgatgatcg tgaccacgac gtacgtgtca agcgcgccga gcaacacggc gagtgcaccc
     2401 gcgctgatcg cgattctgcg gctccggctg gaatgcaccg gtgctgcggc cgtcatcaaa
     2461 cggcgggttt ggtgacggtg acgggcttgt cccaatccga cagcgtcatc tggacgctgg
     2521 tgccgtccga cggcgccagc ttggcctgga cgagcttgtg gtcgccgtcc ttctcgatcc
     2581 aggcggtgcc cggcaccggc ccgctcgctt tgatcggcgg ggcgatcgcg ttcaccgcgt
     2641 cggcgctgac ctgaccggtc accttcacgg tctcgacccc gttgacggtc tcggtgctct
     2701 ccgacttcgg atcgctgaag tttgcgagca cgttggccag gcctttgtcc gggctcaaga
     2761 tggccgagat gtcgtagacg tcagcggccg ggcccatgtc gatgtagctg tcgccggaga
     2821 gtgcgacata gagattcgag tcgatgacga cgaacttcgc gtccacatcg ctgccctgaa
     2881 aggtcagctt ggtatttccc tgacccgcga cggcgggcgc ctgagtcaaa tcgccgctga
     2941 gggttttgat cgggaaatta ttgatctgac cttcgaccgt caagtcgagg tgaatgctct
     3001 tcagcgcacg cgtcgaggcg gtggattctt tgagcaacgt ggcggcatcg ggcagtgatt
     3061 cattcgattt ctccgaggac gacgaacatc ccgcaatgag gactattgct gcgaaaagtg
     3121 cggtgaatat tggccaaagg gatcggactg cgttttttgg gcgcgtctgc ataagtgcat
     3181 cgtagagggt ggcgctgcgt accctgattg gcgtgttcac cggaatcgtc gaagagcttg
     3241 gcgaagtcgt cggcaaggaa gacctcgcgg acgccgcccg gttcgtgatc cgcgggccgg
     3301 tcgtcaccgc ggatgcccgc cacggcgact ccatctccgt caacggtgtc tgtctgacgg
     3361 tggtggaggt gcaggcaggc ggcgaattca gcgccgacgt catgaaggag acgctggacc
     3421 gctcgagcct cggcggcgtc ggcgtcggca gcaacgtgaa cctcgaacgg gccgccgcgg
     3481 tgaacagcag gctgggcggg cacatcgtgt cgggccacgt cgacggcacc ggacatgtga
     3541 tcgtgcgcac accttccgag cactgggagg tggtgcggat agcactgccg accgcactgt
     3601 cgcgctacgt cgtggagaag ggctcgatca cggtcgacgg cgtgtcgttg accgtctcgg
     3661 gcctggggca cgactggttc gaggtgtcgc tgatcccgac gacactgcag ctgacgacgc
     3721 tggggcgggc ggcggtcggc acatccgtca acctcgaggt cgacgtcatc gcgaagtacg
     3781 tcgagcggct gatgctggac agagactgag agaagtgcca attctcagcg ctcggaattg
     3841 gcgtgcgcga taatagctgc gggccgggtc tagcaaaagg atgcattgaa tgaagaagct
     3901 ggcagcagcg cttttgacgg ccaccgcggc ggcggtcgca ccgattgcca tcgcgccggt
     3961 ggcacaggcc gacatctgcg cgggcgcgca cggccgccac gtcggcgtgg gtggctgcac
     4021 gaatatcgcc ggtgacgtgg cgaccggagt cgccgtcgcc tcggcggcag atccctacgt
     4081 gcccggcgag gtgccgtgtt acacggtcga gggtgtccct tattacaccc ctccgggcga
     4141 cccctgctga caggcgcgca gcagccagtt ggcaataatt gccacttcgt cgtggttcat
     4201 actggaggcg gcgacgatgg ttccgaccgg aaccggcaag ggtggcgaag atgacgaggt
     4261 tggactccgt cgatcgggcg gttgccgaca tcgcggaggg taaggccgtc gtcgtcatcg
     4321 acgatgagga ccgcgagaac gagggcgacc tcatcttcgc cgccgagaaa gcaacccccg
     4381 aactcgtcgc gttcatggtt cgctacacct cgggctatct gtgcgtgccg ctcgacggtg
     4441 cgatctgcga caagctcggc ctgttgccga tgtacgccgt gaaccaggac aagcacggca
     4501 ccgcctacac cgtcaccgtc gacgcgaaag agggtgtcgg caccggcatt tcggcatcgg
     4561 accgcgcgac gaccatgcgg ctgctggcca atccgaacag cgtcgccgag gagttcaccc
     4621 ggccgggcca tgtagttccc ctgcgggcca aggacggtgg ggtgttgcgt aggcccggcc
     4681 acaccgaggc cgccgtcgac ctcgccaggt tggccggtct gcagccggcg ggcgcgatct
     4741 gtgaaatcgt cagccagaag gacgaaggtg cgatggcgca gaccgatgaa cttcgggtct
     4801 tcgccgatga acacgacctc gcgctgatct cgatcgccga cctcatcgag tggcgccgca
     4861 agcacgagaa gcacatcgag cgcgtcgccg aggcgcgcat tccgacgcgg cacggcgagt
     4921 tccgcgcggt cggttacacc agcatctatg aagacgtcga gcacgttgcc ctggtccgcg
     4981 gcgacatctc ggggccggag agcgacggac acgacgtcct ggtccgcgtg cactccga
//
LOCUS       DASYLX010000126         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1870829,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000126 DASYLX010000000
VERSION     DASYLX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870829"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..927)
                     /locus_tag="VGP27_03025"
     CDS             complement(127..927)
                     /locus_tag="VGP27_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7850323.1"
                     /translation="MGDVERKPTGKTEAAVQSVDRALLVLEILATLGHAGVTEIAAEL
                     GVHKSTVSRLIAVLESRGYVEQATERGKYRLGFTISRLARASSGHLDLVKLSQDVCDM
                     LAPEVGETTNLAILDEDRIVNIVETIGPEQISLRTWVGQSCPAHATSSGKVLLAGLDA
                     QDLRARLAATLESFTANTVVTLADLERELAGVRERGWACVVEELEVGLNAVSAPIYDA
                     GSNVIAALSVSGPAYRLSPDRFPEIAKQTTAAAEVISRRLGWVERDRG"
     gene            complement(939..2711)
                     /locus_tag="VGP27_03030"
     CDS             complement(939..2711)
                     /locus_tag="VGP27_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEV7850324.1"
                     /translation="MSDDELGDLAAGSELVEFPAGAVVADYATRVPGDVWMVRSGQVT
                     LQASADGTTIDTVEPGGIFGYTPLLTGGGMEFVARAAQASALIRLPGALVRAQFAKPA
                     GLAFLASTAWNVSRADRPTIAPTTDSRPVGEMVHGDVLLVEPDISVRDAVCRMAERHV
                     SYALIRLSDGDMGIFTDRDLRTRVVAAGVSIDAPITDVMSAPARRVTADLTAETVLMD
                     MLECGMKHMPVVTSRGEIVGVLEDADLLAASARQSFSLRRSIGLAADATELQHVGQRV
                     TGVAADLFRNGTKASATSAILSVVLDSLVRRALELALAEAGAEIDGFAWLTLGSVARR
                     EAMPSSDVDSALSWRDDMSPAGDRLRAIAVRTHEILDGCGLPSDRNGAVAYEPLFSRS
                     QSQWHAAAEGWLADPLRGQGLMMSSLLIDGRVVWGDPALHTVPAAYRSMRDEHSDALR
                     LQRLDALAARVKTRSLRDVLSRRGGTFDLKHHAITPIVNLARWGGLSAGVGSASTPAR
                     LSAAAQAGAISDRDAETLCDVFVMLQRLRLAHQVTQIAAGHTPGDVITMSELSPLNRS
                     LLNDGLREIVGVQRRVRNVGILPG"
     gene            complement(2812..4359)
                     /locus_tag="VGP27_03035"
     CDS             complement(2812..4359)
                     /locus_tag="VGP27_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850325.1"
                     /translation="MAEDASPHGRDAGTFDYVIAGGGTAGCALAARLSEDPDVTVCLL
                     EAGPSDVGDDNILMLSEWMHLLDSGYDWDYPVEPQERGNSFMRHARAKVLGGCSSHNS
                     CIAFLPPAECLDEWVTMGADGWSAAEVLPLTKRLKETVLLRDVPPEDPCGAAVLEAAA
                     MVGMPTVAFNRGQTVRNGAGWFQINAAEDGTRMSSSHAYLHPILASRRNLEVRTGCWV
                     AEIVFDDARSATGVRYQRPDLTGHDTVSARREVVVTAGAIDTPKLLMLSGIGPSVHLR
                     EIGIDVRVDSPGVGSNLDDHVEGLVFWEASKPMVTTSTQWWEIGLFTTVDDGRSQADL
                     MMHYGSVPFDMNTLRRGYPTTDNGFCLTPNVTQGRSRGTVRLRSRDFRDRPRVDPRYF
                     TDPEGYDDRIMLTGVRLARRIAEEAPLAAWVGRELAPGPDATTDDELLDYIHQCHNTV
                     YHPAATARMGSVSDPMAVLDPQLRVKGTHRLRVVDASAMPKLPSVNPNITVMTMAEKC
                     ADLIRQC"
     gene            complement(4361..>5426)
                     /locus_tag="VGP27_03040"
     CDS             complement(4361..>5426)
                     /locus_tag="VGP27_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7850326.1"
                     /translation="VFTTVINALGVRLMARINSAGVFIELIAAVLIAIILALNVKRGP
                     DIFFSTEGYGAGESYGYLGAFLVASLASLYVMYGFDTASSLGEETVEPRRTAPKAIFR
                     AILASFVIGGAILVFAVMSAPNLQDPQIGESSGGLQYIVEQVMWGPLGKIFLICIVVA
                     VTVCTLAVHTAAIRLSFAMARDNALPFGEKLARVNPKTQTPIIPAVVIGLIAAIILVI
                     NIGQPKIFTVLTSIAIIMIYLAYLMVTGPMLKKRLQGQWPPKDLKEGGYFTMGRWGLP
                     VNIFAVAWGALMALNLAWPRADVYGEPWYNTWGAFVYIGVILGSGLLWYFIKGRHHIG
                     TLESHAAATVTEAEKPLEQA"
BASE COUNT          932 a         1866 c         1753 g          875 t
ORIGIN      
        1 tcaacgcgcc gtcggactcc tggcgcaaca tcctggcggt gatcacaggc gattccccag
       61 ccctgcacag ggcggtgtcc tagtgacgca acacgatccg ctgttcggcg cgcgagtcgg
      121 ccgagctcat ccccgatcgc gttcgaccca gccgaggcgc cggctgatca cctcggcggc
      181 ggcggtcgtc tgtttggcta tctccgggaa tcggtccggg ctgagacgat aggctggacc
      241 cgagacgctc agcgcggcaa tgacattcga accggcatcg tagatcggag cggatacggc
      301 gttcaggccg acttcgagtt cctcgacgac gcaggcccag ccgcgctcac gcactccggc
      361 gagttcacgc tcgagatccg cgagcgtcac gaccgtgttg gcggtgaacg attccagcgt
      421 cgcggcgagc ctggcacgga gatcctgggc gtcgagcccg gcgagcagta ccttgccgct
      481 cgatgtcgcg tgcgccgggc agctctgacc gacccaggtc cggaggctga tttgttcggg
      541 accgatcgtc tccacgatgt tgacgatgcg gtcctcgtcc aggatcgcga ggttggtcgt
      601 ctcccccacc tccggtgcaa gcatgtcgca aacgtcctgg ctcagcttca ccagatcgag
      661 gtgaccgctg ctggccctcg ccagccggga gatcgtgaaa ccgagccgat acttgccgcg
      721 ctccgttgcc tgctcgacgt agccacggga ctccagaacg gcgatcagcc gcgacacggt
      781 tgacttgtgc accccgagtt cggcggcgat ctcggtgacg ccggcgtggc ccagtgtcgc
      841 caagatctcg agcaccagca gcgcgcggtc aacggattga actgcggcct ctgtctttcc
      901 agtgggcttg cgctccacat cccccatggg tcgccaccct atccggggag gattccgacg
      961 ttgcggaccc gtcgctgcac accgacgatc tcgcgcagcc cgtcgttgag caggctgcga
     1021 ttcagcggcg aaagctcgga catggtgatc acgtcgccgg gcgtgtgacc ggcggcgatc
     1081 tgggtgacct gatgagccag ccgcagccgc tgcagcatca cgaagacgtc gcacagcgtt
     1141 tcggcatcgc ggtcgctgat cgcacccgcc tgtgctgccg cgctgagccg ggcaggcgtc
     1201 gacgcggagc cgacgcctgc gctcagccca ccccagcgtg caaggttcac gatcggggtt
     1261 atcgcgtggt gcttgaggtc gaatgtgcca ccgcgccgcg acagcacatc acgcagcgat
     1321 cgggtcttga ccctggcggc cagcgcgtcg agccgctgca gccgcagggc gtcggagtgt
     1381 tcgtcgcgca tcgaacgata ggcggcggga acggtgtgca gcgccggatc tccccagacc
     1441 acccgcccgt cgatgagcag cgacgacatc atcagaccct gaccgcgcag cggatcggcg
     1501 agccagcctt ccgccgccgc gtgccactgt gactgcgaac gcgagaagag cggctcgtat
     1561 gcgaccgcgc cgttgcgatc cgagggcaaa ccacagccgt cgagaatctc gtgtgtgcgg
     1621 acggcgatcg cacgcaatcg atcaccggca ggcgacatgt cgtcgcgcca cgacaacgcg
     1681 ctgtccacat cggaagacgg cattgcctcg cggcgggcga cgctgccgag cgtcagccag
     1741 gcgaatccgt cgatctcggc accggcctcc gccagcgcca gttccagcgc cctgcgcacc
     1801 aggctgtcga ggaccaccga caggatcgcg ctggtggccg atgccttggt gccgttgcgg
     1861 aacaagtcgg cggccacccc ggtgactcgc tgtccgacgt gctgcaattc ggttgcgtcg
     1921 gcggccaatc cgatggagcg gcgcaggctg aagctctgcc gggccgatgc ggccagcagg
     1981 tcggcgtctt cgagaacgcc gacgatctca ccgcgcgacg tcacgaccgg catgtgcttc
     2041 atgccgcact ccagcatgtc catcagcacc gtctcggcgg tcaggtccgc ggtgacgcgc
     2101 cgcgcgggcg cactcatcac gtcggtgatc ggcgcgtcga tcgaaacacc ggcagcgacc
     2161 accctggtcc gcaggtcgcg gtcggtgaaa atgcccatgt ccccgtcgga cagccggatc
     2221 agggcgtaag agacgtggcg ctcggccatc cggcacaccg cgtcgcgcac cgagatgtcg
     2281 ggttcgacaa gaagcacgtc accgtgcacc atttcgccga ccggcctgct gtcggtagtc
     2341 ggcgcgatcg tcggacggtc cgcgcggctg acgttccacg ccgtcgacgc caggaacgcc
     2401 aggcccgccg gcttggcgaa ctgcgcgcga accagcgcac ccggcagccg gatcagcgcg
     2461 ctcgcctgcg cggcccgcgc cacgaattcc attccgccac cggtgagtag cggggtgtag
     2521 ccgaagatgc cgccgggctc gacggtgtcg atcgtggtgc cgtcggcgct cgcctgcagt
     2581 gtcacctgac cgctgcggac catccagacg tcgccgggca cccgtgttgc gtagtcggcg
     2641 acgactgcac ctgccgggaa ctcgaccaac tccgagccgg ccgccagatc gccgagttcg
     2701 tcgtcggaca tcgcctggaa cggtgtgtgc tcgccgagga acgtttcgag gcggacctcg
     2761 aattcagcac cggggcgagc cccgaaatca gcaccggggc gagccccgaa atcagcactg
     2821 ccggatgagg tcggcacact tctccgccat cgtcatcacc gtgatgttgg ggttcaccga
     2881 cggcagcttc ggcatcgcgg acgcgtcgac gaccctcagc cggtgggtac ccttgacccg
     2941 cagttgcggg tcgagcaccg ccatcgggtc actgacggag cccatccgcg ccgtcgccgc
     3001 cgggtgatac acggtgttgt ggcactggtg gatgtagtcg agcagttcgt cgtccgttgt
     3061 cgcatcgggg cctggtgcca gctcgcgacc cacccacgca gccagcggcg cctcttcggc
     3121 gatcctgcgg gccagccgga cacccgtcag catgatccgg tcgtcatacc cctcgggatc
     3181 ggtgaagtag cgcggatcaa cccgcggccg gtcgcggaag tcgcggctgc gcaaacgcac
     3241 tgtgcccctg gaccggccct gagtcacgtt gggtgtcaag cagaatccgt tgtcggtggt
     3301 gggatagcca cgccgaagcg tgttcatgtc gaacggcacg cttccgtagt gcatcatcag
     3361 gtcggcctgg ctgcggccgt catcgacggt ggtgaacaac ccgatctccc accactgcgt
     3421 ggatgtcgtc accatcggct tcgacgcctc ccagaacacc agcccttcca cgtggtcatc
     3481 gaggttcgac ccaacgcccg gcgagtccac ccggacgtcg atgccgatct ctcgcaggtg
     3541 cacgctcggg ccgattcccg agagcatcaa caacttcggc gtgtcgatcg cgcccgcggt
     3601 gacgacgacc tcacgccgcg ccgagacggt gtcatggccc gtcaggtccg gccgctgata
     3661 gcggacaccg gtggccgaac gtgcgtcgtc gaagacgatc tcggccaccc agcaaccggt
     3721 gcgcacctcg aggttgcgcc tcgacgccag gatgggatgc aaatacgcgt gcgagctgga
     3781 catcctggtg ccgtcctcgg ccgcgttgat ctggaaccaa cccgcgccgt tgcggacggt
     3841 ctgcccacga ttgaaggcga ccgtcggcat gcccaccatc gcagccgcct ccagaaccgc
     3901 tgcgccgcag gggtcctcag gaggcacatc gcggagcaac accgtttcct tcaaccgctt
     3961 ggtcagaggc aggacttcgg cggcgctcca gccgtcggcc cccatggtca cccactcgtc
     4021 aaggcattcc gcgggcggca ggaacgcgat gcacgagttg tgtgatgagc agccgcccag
     4081 cacctttgcg cgcgcgtgcc gcatgaacga gttgccccgc tcctgcggct cgaccggata
     4141 gtcccagtcg taacccgaat ccagcagatg catccactcc gacaacatca ggatgttgtc
     4201 gtcgccgacg tcggagggcc cggcctcgag caggcacacc gtgacgtccg gatcctccga
     4261 gagccgggcg gccagcgcac agcctgcggt gccgccaccg gcgatcacat agtcgaaggt
     4321 gccggcgtcc cggccgtgag gagatgcatc ctcagccatg tcaggcctgc tcgagcggct
     4381 tctcggcttc ggtcaccgtc gccgcggcat gcgactccag cgtgccgatg tggtgacggc
     4441 ccttgatgaa ataccagagc agaccggatc cgaggatcac accgatgtag acgaacgcgc
     4501 cccaggtgtt gtaccagggt tcgccgtaca cgtcggcgcg cggccacgca agattcagcg
     4561 ccatcagggc gccccaggcc accgcgaaga tgttgaccgg aagcccccag cgacccatcg
     4621 tgaagtagcc gccctccttg agatccttcg gcggccattg cccttgcagg cgcttcttca
     4681 gcatcggccc cgtcaccatc aggtaggcga ggtagatcat gatgatcgcg atcgaggtca
     4741 gcactgtgaa gatcttgggc tggccgatgt tgatcaccag gatgatcgcc gcgatcagac
     4801 cgatgacgac tgccgggatg atcggcgtct gtgtcttcgg gttcacccgg gcgagcttct
     4861 cgccgaacgg aagagcgttg tcgcgggcca tcgcgaacga cagccgaatc gcggccgtat
     4921 gcaccgcaag cgtgcagacc gttactgcca ccacgatgca gatcaggaag atcttgccga
     4981 gcgggcccca catcacctgc tcgacgatgt actgcaggcc gccgctgctc tcgccgatct
     5041 gcgggtcctg caggttcggc gcggacatca ccgcgaagac caggatcgca ccgccgatga
     5101 cgaacgaggc gaggatggcc cggaagatgg ccttcggcgc cgtgcggcgc ggttcgacgg
     5161 tctcctcacc cagcgagctt gctgtgtcga agccgtacat cacgtacagc gatgccagcg
     5221 acgcgacgag gaaggccccc agatagccat agctctcgcc cgcgccgtag ccctcggtcg
     5281 agaaaaagat gtcgggcccg cgcttgacgt tcaaggccag gatgatggcg atcagcacgg
     5341 cagcgatcag ctcgatgaac acgcccgcac tgttgatcct ggccatcagc ctcaccccga
     5401 gagcattgat caccgtggtg aatacg
//
LOCUS       DASYLX010000127         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_664368,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000127 DASYLX010000000
VERSION     DASYLX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664368"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VGP27_03045"
     CDS             <1..573
                     /locus_tag="VGP27_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850327.1"
                     /translation="DPGLRPECGGIVSGAAMGGSDMRIGFRAVQGVQIRFAESNSPTS
                     PRLVLTSPWPESLLAFQRVWPPRFSRTAHLLAVDLPGFGHSECRTELLGDRFARATRY
                     VRSYPEQLQRLGQILSTIFTPVQIIAGRWDPLVPLANADYLHARLPNSHLDVLDTGHF
                     AWEQAPDHYAAIVTAWVNGGYLTGSAGLQH"
     gene            635..1384
                     /locus_tag="VGP27_03050"
     CDS             635..1384
                     /locus_tag="VGP27_03050"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7850328.1"
                     /translation="MSGLTGKVAVVTGGSSGIGQAIAQRFVAEGAYVFITGRRQSALD
                     KAKALIGKNVTTVRGDATSTADLDRLFETVLQEKGKLDILVANSGRVEPAEFGKITED
                     DFDKTFDLNARATLFTAQKALPLMRDGGSIVLVGSIAGHKGLNGYTTYSATKAALRSY
                     ARTWTQEFKDRGIRVNTLSPGPIDTPIMDGQADTKEGADAIRATFASMIPLARMGQPE
                     EVAAAAYFLASEESSFVTGIELAVDGGMAQV"
     gene            1485..1769
                     /locus_tag="VGP27_03055"
     CDS             1485..1769
                     /locus_tag="VGP27_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850329.1"
                     /translation="MSTRGDRNVGVRHGFNDRIADTTYRWHDDSVNFTHENDIKANFA
                     EIMKGQFDTQEMISIIGADDGKYRNVWPPAIEDLVKQVQDAAWTRPVKHS"
     gene            1930..>2502
                     /locus_tag="VGP27_03060"
     CDS             1930..>2502
                     /locus_tag="VGP27_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV7850330.1"
                     /translation="MADTHVIVLPGGGYATHARNEAEPIVAWLSGLGVASSVFRYPLN
                     VPHPVPLGALRAEIRRRRSNGADRIGLIGFSAGGHLAGLAALAPGAEPAVAVQFVIVG
                     YAITSMETETYRPARLILLGEGASPELRRATSLDSLVTPHSPPFFLWHTAEDIYVPPE
                     HTYRVASALAAHGVAHAVHVFAHGPHNLGLA"
BASE COUNT          457 a          867 c          764 g          414 t
ORIGIN      
        1 gatcctggtc ttcgtccaga gtgcgggggc atcgtgagcg gagcagcaat gggcggcagt
       61 gatatgcgga ttggttttcg ggctgtgcag ggggtgcaga tccgctttgc tgaaagcaac
      121 agccccacca gcccccgcct cgtactgacc agtccctggc cggagagcct gctggccttt
      181 cagcgggtct ggccaccacg gttttccagg acagcccacc tcctcgcggt cgacctgccc
      241 ggcttcggcc actccgaatg ccgaaccgag ctcctcggtg accggttcgc gcgggccacc
      301 cgctacgtgc gtagctatcc cgaacaactc cagcgcctcg gccagatcct ttccacgatc
      361 tttactccgg tgcagatcat cgctggccgc tgggaccccc tggtgcccct ggccaacgcc
      421 gactatctcc atgcccgcct gcccaacagc cacctcgacg tgctcgacac cggccatttc
      481 gcctgggaac aggcacccga tcactacgcg gcgatcgtca ccgcctgggt caacggcggc
      541 tacctcaccg gaagcgcagg actccagcac tagccacaac ccgcccccga ctcactttga
      601 gcaacacccg cagcaggact cgaaaggact agacatgtct ggattgaccg gaaaggtggc
      661 cgtcgtcacc ggcggaagca gcggcatcgg ccaggccatc gcgcagcggt tcgtcgccga
      721 aggcgcctac gtcttcatca ccggccggcg gcaatccgcg ctggacaagg cgaaggccct
      781 gatcggcaaa aatgtgacca ccgtgcgcgg tgacgccacc agcaccgccg acctcgaccg
      841 cctcttcgag acagtcctcc aggagaaggg caagctcgac atcctggtcg ccaattccgg
      901 tcgcgtcgag ccggccgagt tcgggaagat caccgaggac gacttcgaca agaccttcga
      961 tctgaacgcc cgggcaacgc tgttcaccgc gcagaaggcg ctacccctca tgcgagacgg
     1021 agggtcgatc gtcctggttg gctcgatcgc tggccacaag ggcctcaacg gctacaccac
     1081 ctacagcgcg accaaggcgg cattgcgctc ctacgcccgc acctggaccc aagagttcaa
     1141 ggatcgcggc atccgggtca acaccctgag cccggggccg atcgacaccc ccatcatgga
     1201 cggccaggcg gacacgaagg aaggcgccga cgccatcagg gcaaccttcg cctcgatgat
     1261 cccgctcgcc cgaatgggcc aacccgagga agtagccgcc gccgcctact tcttggcctc
     1321 cgaggagagc agctttgtca caggcatcga gctcgccgtc gatggcggca tggcccaggt
     1381 ctaatccaac gcccgacggg aaccccttcc gcaaggggcg cccgtctgcc gtcgcgagtg
     1441 gagtcactct cagcgtcaac ttcgataaca cggcgccaga tgccgtgagc acccgcggcg
     1501 accgaaatgt tggcgtacga cacggcttca acgatcgcat cgccgatacg acctaccggt
     1561 ggcacgacga ttcggtcaac ttcacccacg agaacgacat caaggcaaat ttcgccgaga
     1621 tcatgaaggg tcagttcgat acccaggaga tgatctctat catcggcgcc gacgacggga
     1681 agtaccgcaa cgtctggccg ccggcgatcg aggacctcgt caagcaggtc caggacgccg
     1741 cgtggactcg gccggtcaag cacagctaag agacaccccg gcgtctaaca cacagtgcgg
     1801 tcgtcgcgct cggacagcag gactctgtca gccgccgcgc agcaggaagg aaccgttccc
     1861 gatgactagc gtccccggga ttggcttcga ttgcatcaac gcgtctgtcg tggtggagga
     1921 ggaccgttga tggcagacac gcacgtgata gtcctgccgg gcggcgggta cgcgacgcat
     1981 gcccggaacg aggccgagcc gatcgtcgcg tggcttagcg ggttgggggt tgcgtccagc
     2041 gttttccgct acccgctcaa tgttcctcac ccggtgcccc tcggcgcgct gcgcgccgag
     2101 attcggcgtc gtcgcagcaa tggggctgac cgcatcggtc tgatcggctt ctcggccggc
     2161 ggtcacctgg ccgggcttgc tgccctggcg cccggtgccg agccggctgt cgcggtgcag
     2221 ttcgtgattg ttggctacgc gatcacctcg atggagaccg agacctaccg tccggccaga
     2281 ctgatcttgc ttggcgaagg cgcgagcccg gagttgcggc gcgcgacctc tctggactca
     2341 ctggtgactc ctcactcgcc gccgttcttc ctctggcaca cggcggagga catctatgtc
     2401 cctcctgagc acacttatcg cgtcgcgtcg gcgctggcgg cccacggggt tgcgcacgcg
     2461 gtccatgtat tcgcgcacgg gccgcacaat ctcggcttgg ca
//
LOCUS       DASYLX010000128         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3626304,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000128 DASYLX010000000
VERSION     DASYLX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3626304"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VGP27_03065"
     CDS             <1..956
                     /locus_tag="VGP27_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7850331.1"
                     /translation="TCLAAARHAVLAAAGWNVEDDGLIGAPPVRILCGEAAHATIYTA
                     LRQLGLGANTAIAVAADDQGRMRPDALRDALATSAGPAIVCAQAGNVATGAFDDFEAI
                     ADACADHDAWLHVDGAFGLWAAAAPALRHLTKGVERADSWAVDAHKWLNVPYDGAMAI
                     VADSNAHVAAMGLAGPYLVADPGQRDGTNYVPESSRRARVIPIYAALRSLGRAGVAEL
                     IERNCAQARRMAERLAEIPGARIVNDVVLNQVLVALPGGDDANRAAVAAVQRDGTCWL
                     GGTTWNNRYLLRVSVSNWATTDVDIDRSAGAIARAAAAVQG"
     gene            1076..1447
                     /locus_tag="VGP27_03070"
     CDS             1076..1447
                     /locus_tag="VGP27_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850332.1"
                     /translation="MTSTKAMTLTSVAFAPLAIAAIALVGAAPAVAATPAAHQQIVAT
                     AAVSDYSLWNDGEPAGGTGIDPGPQTAPDIPGIPDQAGVDSSDLGLRAGGTGIDPGPS
                     APADTPSGPGLEDGGEPAGAV"
     gene            complement(1444..1719)
                     /locus_tag="VGP27_03075"
     CDS             complement(1444..1719)
                     /locus_tag="VGP27_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850333.1"
                     /translation="MLIEQYLDFVTNRMFPESLLVHAERTPQGTYNLHGGRYEHLHFA
                     ASVPPVDGPTRPLPANRFGHSDRRDRERCEVAPKPQLIRTELGGPLF"
     gene            complement(1755..2528)
                     /locus_tag="VGP27_03080"
     CDS             complement(1755..2528)
                     /locus_tag="VGP27_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7850334.1"
                     /translation="MSLVTYALEDHVATITLNRPEARNAINGELRRDLNAAWDRFRDD
                     EDAWVGVLTANGDVFCAGGDLKDGEASVGSFGGTFWEKPTINSFESGMELFKPTIAAV
                     HGPCIGYGLTGVLFCDFVIASTEAKFCFPEVGIGVPTIVGAIRLPQRVGWANAMELLL
                     TGKPMSAERAKEVGLVWKLVAPDGLQSEAQAWARTLTEAAPLAQRATKEVAWRSANMG
                     WIEAVRFGETMRKVAGATEDVAEGIGAWREKRAPHWKGR"
     gene            2640..3038
                     /locus_tag="VGP27_03085"
     CDS             2640..3038
                     /locus_tag="VGP27_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEV7850335.1"
                     /translation="MSVDRAALAVGGIRMASGISFLVDPLRANRLWGDPDEPPPTAQL
                     LLRSMGYRDALIGGLLMSAALRHKNTRGWFLASGGADAADLLGGMSVHHDMKRSQQLI
                     GLGGAVVGIGVGLWGATRRRRDFATAPEER"
     gene            complement(2992..3612)
                     /locus_tag="VGP27_03090"
     CDS             complement(2992..3612)
                     /locus_tag="VGP27_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850336.1"
                     /translation="MKEAVDAEAILDTAARLIELDGVDAFTMRGLAESLGVAVTSIYW
                     HIGGRDKLFDSLVDRLLSHMAHLPVDGERPVDRIASLARAQRTMLIERQHLLAIAHER
                     DRTPLLFLPIQQTLAAQLAEVGVTGTDAALILRAIQVHVISSAVMQFSAVRGAKHDEE
                     DPSLWTDGWPDRALVEALQSPTDYDAVFEYGLNALLAPLRNRACGG"
     gene            complement(3609..>4154)
                     /locus_tag="VGP27_03095"
     CDS             complement(3609..>4154)
                     /locus_tag="VGP27_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850337.1"
                     /translation="YATTQGALMGVADGTPFPSDHRRPDKSVSDVIAERTKAFAAERG
                     VDLGWASTDQLMRLHQYNVFPNMSLLANADHLTVLASRPGPDPDHGELVMILWTRMSP
                     GAPRIKPADMRMTTDEAHPGVVLSQDIAVLAGLQRGLHQPGLTHLTLSNEERRVINTH
                     RNLERYLDLPESERMTGGETP"
BASE COUNT          659 a         1378 c         1403 g          714 t
ORIGIN      
        1 caacctgtct ggccgccgcc cgccacgcgg tgctcgcggc ggccggctgg aatgtcgaag
       61 acgacgggct catcggtgcg ccgccggtac gcatcctgtg cggtgaggcg gcccacgcga
      121 cgatctacac cgcgctgcgc cagctcggac tcggcgccaa cacggccatc gccgtcgccg
      181 ccgacgacca gggacggatg cggccggacg cgctgcgcga cgcgctggca acatcggcgg
      241 gcccggcaat cgtctgtgcc caggcgggca acgtggccac cggagccttc gacgacttcg
      301 aagcgatcgc cgatgcctgc gccgaccatg acgcctggtt gcacgtcgac ggcgcgttcg
      361 ggctgtgggc cgccgccgcg cctgcgttgc ggcacctgac gaagggagtc gaacgcgccg
      421 actcgtgggc cgtcgacgcc cacaagtggc tgaacgtgcc ctacgacggc gcgatggcca
      481 tcgtcgccga ctcaaacgcc cacgtcgccg cgatgggcct cgcggggccg tatctcgtcg
      541 ccgatcccgg gcagcgcgac ggcacgaact atgtgcccga gagttcgcgg cgggcgcgcg
      601 tcataccgat ctatgcggcg ctgaggtcgc tgggccgtgc cggggtcgcc gaactgatcg
      661 aacgcaattg cgctcaggcg cgccgaatgg ccgaacggct tgcggagatc cccggcgcgc
      721 ggatcgtcaa cgacgtcgtg ctgaaccagg tgctggtcgc cctgccgggc ggcgatgacg
      781 ccaacagggc cgcggtggcc gccgttcagc gcgacggcac ctgctggctc ggcggcacca
      841 cctggaacaa ccggtatctg ctccgggtct cggtctcgaa ctgggcgacg accgacgtcg
      901 acatagaccg ttcggccggc gcgatcgccc gtgccgccgc tgccgtgcag ggctgagatt
      961 tccggctggc aggaggtcgg ttttggtgtc tgcagtgacg actttgcggg cgctgagcgc
     1021 gatcgtttaa ggcatgcggc gatcgggcgc cgttcatgtc gaccaggagc ccaccatgac
     1081 aagcacaaaa gcgatgacac tcacttctgt ggcgttcgca cctctcgcaa ttgcggccat
     1141 cgcgcttgtt ggcgccgcgc cggccgtcgc ggcgactccg gctgcacacc agcagattgt
     1201 cgccaccgcc gctgtgagcg actactcact atggaacgac ggcgagcctg ccggcggcac
     1261 gggaatcgac ccagggcccc agaccgcccc tgacatccca ggcattcccg accaggccgg
     1321 tgtggactcc tcggaccttg gactgcgcgc cggcggcacc ggcatcgatc caggcccgtc
     1381 ggcgccggcg gacaccccgt ccggccccgg cctggaggac ggcggcgaac ccgcgggcgc
     1441 ggtctagaac aaaggacctc cgagttcggt gcgaatcagt tgtggcttcg gcgccacttc
     1501 gcagcgctcg cgatcccgcc ggtcgctgtg gccgaaccgg ttggccggta gcgggcgcgt
     1561 cggcccgtct acgggcggca ccgatgcagc gaaatgcaga tgctcgtaac ggccgccgtg
     1621 caggttatag gtgccctggg gcgtgcgttc ggcgtggaca agcagcgatt cgggaaacat
     1681 gcggttcgtc acgaaatcca ggtactgctc gatcagcacc tggacgcctg atggccgacc
     1741 atttcgcgcg ggtgtcagcg tcccttccag tgcggtgctc gtttctcgcg ccatgcgccg
     1801 atgccttcgg ccacgtcctc ggtggccccg gccaccttgc gcatggtctc gccgaagcgc
     1861 accgcctcga tccaacccat gttcgcgctt cgccatgcga cttccttggt ggcgcgttgg
     1921 gccaacggcg cggcctcggt gagcgtccga gcccatgctt gggcctcgga ctgcaaccca
     1981 tcgggcgcaa cgagcttcca caccaggccg acctcttttg cccgctcggc gctcatcggc
     2041 ttgcccgtca gcagcagctc catcgcgttg gcccagccga cgcgctgcgg aaggcggatg
     2101 gcgccgacga tggtggggac accgatcccg acctccggga agcagaattt ggcctcggtg
     2161 ctagcgatga cgaagtcgca gaacagcact ccggtaagcc cgtaaccgat gcaaggcccg
     2221 tgcaccgccg cgatggtcgg tttgaatagc tccatcccgc tctcgaacga gttgatcgtc
     2281 ggcttctccc agaaggtgcc gccgaacgag ccgaccgatg cctcgccgtc cttgagatca
     2341 cctccggcgc agaagacatc gccgttggcc gtcagtactc cgacccaagc atcctcgtcg
     2401 tcgcggaagc ggtcccaggc cgcgttcaag tcgcggcgaa gttcaccgtt gatcgcattg
     2461 cgcgcttcgg gtcggttgag cgtgatggtg gcgacgtgat cttccagcgc gtacgtgacg
     2521 aggctcatga atgcacccta tttggcgcgt ccgatgtcga atagtgaagt gctcgcgttc
     2581 gatttcttca gacgttacga accaatcgat tcgagcgcgg ggcgcctagg ctgcatgcca
     2641 tgtccgtaga tcgcgccgca ctcgccgtcg gtggtatccg catggcgtcg ggaatatcct
     2701 ttctggtcga tccgctccgc gcgaaccggc tctggggcga ccccgatgag ccgcccccga
     2761 cggcacagct gctcctgcgg tcgatgggct accgcgacgc acttatcggc ggcttactga
     2821 tgtcagcggc gttgcgccac aagaacactc gcggctggtt cctcgcctcg ggcggggccg
     2881 acgcggcgga tctgctgggc ggtatgagcg tccaccacga catgaagcgc tcccagcagc
     2941 tgatcgggct cggcggcgcc gtcgtcggca tcggagtcgg attgtggggc gctacccgcc
     3001 gcaggcgcga tttcgcaacg gcgccagaag agcgttgagc ccgtattcga atacggcgtc
     3061 gtagtcggtc ggagattgga gcgcctcgac gagtgcacgg tcgggccagc cgtcggtcca
     3121 cagcgaagga tcttcttcgt cgtgtttggc accacggact gccgagaact gcatgacggc
     3181 agaggagatc acgtgaacct gtatcgctcg cagaatgagc gccgcatcgg taccggtgac
     3241 acccacctcc gccagctgcg cggccagcgt ctgctggatc gggaggaaca acaggggcgt
     3301 tcggtcacgt tcgtgtgcga tggcgagcag gtgttgtcgc tcgatcagca tcgtgcgctg
     3361 ggcgcgggcc agagacgcga tgcggtcaac ggggcgctca ccgtcgacgg gtagatgcgc
     3421 catgtggctc agcaatcgat cgaccaggct gtcgaacagc ttgtcccggc ctccgatatg
     3481 ccagtagatc gaggtgaccg caacgccgag gctttcggcg agaccgcgca tggtgaaggc
     3541 atccaccccg tcgagctcga tcaagcgagc cgcggtgtcg aggatcgcct cggcatcgac
     3601 cgcttctttc acggtgtttc acctcctgtc atccgctcgg attccggcag gtccagatag
     3661 cgttccaggt ttcggtgggt gttgatgacg cgtcgttcct cgttggagag tgtcaggtgg
     3721 gtcaggccgg gttggtgcag accgcgctgc aggccggcga gcaccgcgat gtcttgactc
     3781 agcaccacgc cggggtgtgc ctcatcggtt gtcatgcgca tgtcggccgg cttgatccgt
     3841 ggcgcaccgg gactcatccg cgtccacagg atcatcacca gctcgccgtg gtcggggtcg
     3901 ggcccaggac gtgaggccag gaccgtcagg tgatcggcgt tggccagaag cgacatgttg
     3961 gggaagacgt tgtactggtg tagccgcatg agctggtcgg tagacgccca gcccagatcc
     4021 acaccccgct cggcggcgaa agcctttgtg cgttcggcga tcacatcgga aaccgacttg
     4081 tccggccgcc gatgatcgga cgggaaaggg gtgccgtcgg caacacccat cagcgcgcct
     4141 tgggtggtcg cgta
//
LOCUS       DASYLX010000129         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1212692,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000129 DASYLX010000000
VERSION     DASYLX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212692"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGP27_03100"
     CDS             <1..640
                     /locus_tag="VGP27_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEV7850338.1"
                     /translation="VLWTEVNYSDDCGDTWQDAGPDARRLGSHADGRQQLITWEHDPE
                     TGSVYVIASAFNRRENAYLYRVRADDLLDRSAWHPWTGGWFNSDPMPLLPAGTTVGEM
                     SLRRVQNRWVFSYFESNSGTIRVKVLNSPTDDFKAAPTTTVLRNTSWPSPRCSPPDGC
                     TAGTLSQPYGGYIVPGSTLRNLHLVVSHWNTTDGSNWPYRAIQYRHNATPRL"
     gene            complement(642..1457)
                     /locus_tag="VGP27_03105"
     CDS             complement(642..1457)
                     /locus_tag="VGP27_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEV7850339.1"
                     /translation="MNPRNSDCEALRHGELTVIGRIRSASNATFLCEAHLDDLTVHCV
                     YKPVAGEAPLWDFPDGTLAGRERSAYLVSAALGWDIVPYTIIRDGPAGQGMLQLWVDQ
                     PGDEVGENPESGPDLVDLLPAGHIPPGLLPVLRAYDYAGDEVTLVHADDARLRRMAVF
                     DVLINNADRKGGHILSAVDGHVYGVDHGVSLHVQDKLRTVLWGWAGKPVDDETLETVA
                     RLRDDVRGVLGEQLREHITGREVAALRRRAVALLDNPVMPSPDRHRPIPWPAF"
     gene            complement(1441..2022)
                     /locus_tag="VGP27_03110"
     CDS             complement(1441..2022)
                     /locus_tag="VGP27_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEV7850340.1"
                     /translation="MTRAIHVFRTPDRFVAGTVGQPGNRTFYLQAVHDKRVVSVILEK
                     QQVAVLAERIAALLLEINRRFGTPIPPETDVEDLSPLVTPVDAEFRVGTMGLGWDSEA
                     ETVVVELLAVSDTEFDASVVLDDAEEGPDAVRVFLTPESARQFATRSNRVISAGRPPC
                     PLCDEPLDPEGHICVRTNGYRRSLLEPDDEPEE"
     gene            complement(2044..>2538)
                     /locus_tag="VGP27_03115"
     CDS             complement(2044..>2538)
                     /locus_tag="VGP27_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEV7850341.1"
                     /translation="GVEPITDERISEVDYGEWTGRKIGDLVSEPLWAVVQQQPSAAVF
                     PKGEGLAHVQARAVAAVREHDRLLAEKHEGDVLWLACTHGDVIKAIVADALGTHLDSF
                     QRITADPASMSVIRYTAMRPFVVHVNHTGDQLASGLTAKPAEKTEGKSDVPPDDAVVG
                     GSTD"
BASE COUNT          423 a          875 c          825 g          415 t
ORIGIN      
        1 cgtgttgtgg accgaggtca actacagcga cgactgcggc gacacctggc aggacgcagg
       61 gccggacgcc cgtcggctcg gttcgcacgc cgacggccgt cagcagctga tcacctggga
      121 gcacgacccg gaaaccggct cggtgtacgt gatcgccagc gcattcaacc gtcgcgagaa
      181 cgcctatctc taccgggtgc gcgcggacga cctgctggac cggtcggcat ggcatccgtg
      241 gaccggcggc tggttcaaca gcgacccgat gccgttgctg cccgcgggca cgacggtcgg
      301 cgagatgagc cttcgtcgtg tccagaaccg ttgggtgttc agctatttcg agtcgaactc
      361 gggaaccatc cgggtcaagg tcctgaacag tccgaccgac gacttcaagg ccgcgccgac
      421 cacgaccgtg ttacgcaaca cgtcatggcc gtcgccgcgg tgcagtcccc ccgacgggtg
      481 caccgcgggt accctcagcc agccctacgg cggctacatc gtgcccggct cgacattgcg
      541 caacctgcat ctcgttgtca gccactggaa caccaccgac ggttccaact ggccctaccg
      601 cgcaattcag taccggcaca acgcgacccc gcggctgtga ctcagaacgc gggccacggg
      661 atcggtcgat gccggtcggg tgacggcatc accggattat caagcagcgc aacggctcta
      721 cgacgcaagg cagccacctc gcggccggtg atgtgctcac gcagctgctc gccgagcacg
      781 ccacgtacgt cgtcgcgcag tcgggcgacc gtctccagcg tctcgtcgtc gacgggcttg
      841 cccgcccacc cccacagcac ggtgcgcagt ttgtcctgga catgcaggct cacaccgtga
      901 tcgacgccgt agacatggcc gtcgaccgcg gacaggatgt ggccgccttt gcggtctgcg
      961 ttgttgatca gcacgtcgaa caccgccatc cggcgcagcc gggcgtcgtc cgcgtgcaca
     1021 agcgtgacct cgtcacccgc gtagtcgtag gcgcgtagca ccggcagcaa tccgggcggg
     1081 atgtggccgg cgggaagcaa atcgactaga tccgggcccg actcggggtt ctcgccgacc
     1141 tcgtcgccgg gctggtccac ccatagctgc aacatgcctt gtcctgccgg cccgtcgcga
     1201 atgatggtgt aaggcacgat gtcccagccc agcgccgccg acaccagata cgcggagcgt
     1261 tcccggcctg ccagcgtgcc gtcagggaag tcccacagcg gagcctcgcc tgcgaccggc
     1321 ttgtacacgc agtgcaccgt caggtcgtcg aggtgtgcct cgcagagaaa cgtggcgttg
     1381 ctggcggagc ggatacgtcc gatgacggtg agttcgccgt gccgcaacgc ctcgcagtcg
     1441 ctattcctcg ggttcatcgt cgggctcgag caggctgcgc cgatagccgt tggtgcgcac
     1501 acagatgtga ccctctgggt cgaggggctc gtcgcagagc gggcacggcg gcctgccggc
     1561 ggagatgacc cggttggacc gggtcgcgaa ttgacgcgcg gactcggggg tgaggaacac
     1621 gcgcacggcg tcgggccctt cctcggcgtc gtcgagcacg acggacgcat cgaactcggt
     1681 atccgagacc gccagcagct caaccacgac tgtctccgcc tcggaatccc agccgagccc
     1741 catcgtgccg acccgaaact ccgcgtcgac gggcgtgacc aacgggctga ggtcctcgac
     1801 gtccgtctcg ggcgggatcg gggtgccgaa ccggcggttg atctccagca gtaacgccgc
     1861 gattcgttcg gcgagcaccg cgacctgctg cttctccaag atcaccgaga cgacccgctt
     1921 gtcgtgtacc gcttgcaggt agaaggtgcg gttgccgggc tgccccacgg tcccggccac
     1981 gaaacggtcg ggtgtgcgga agacatggat tgcgcgggtc atggcccctc caaaataccg
     2041 cgatcagtct gtggagccgc ccaccaccgc gtcgtcgggc ggcacgtccg acttgccctc
     2101 agtcttctca gccggctttg ccgtcaaacc cgaagcaagt tggtcgccgg tgtgattgac
     2161 gtgaaccacg aagggacgca tcgctgtgta gcggatcaca ctcatcgacg cagggtcggc
     2221 ggtgatgcgc tggaagctgt ccaggtgcgt gccgagcgcg tcggccacga tcgccttgat
     2281 cacgtcaccg tgggtgcagg ccagccagag cacgtcgcct tcgtgcttct cggcgagaag
     2341 acggtcatgc tcccgcaccg ccgcaacggc gcgggcctgt acgtgtgcca ggccctcacc
     2401 tttggggaac accgccgcgc tgggctgctg ttgcaccacc gcccacagcg gctctgagac
     2461 cagatcgccg atcttgcggc cggtccactc gccgtagtcc acctcggaga tgcgctcgtc
     2521 ggtgatcggc tccacccc
//
LOCUS       DASYLX010000130         7941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2419634,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000130 DASYLX010000000
VERSION     DASYLX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419634"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..964
                     /locus_tag="VGP27_03120"
     CDS             83..964
                     /locus_tag="VGP27_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEV7850342.1"
                     /translation="MNTEQYEKMGKGAGFIAALDQSGGSTPKALKLYGIEESAYSNDE
                     QMFDLVHEMRTRIITSPAFDGDRIMGAILFEMTMDRLIDGRPSADYLWNVKGIVPFLK
                     IDKGLADEQDGAQTMKPIPGLDELLDRAVANDVFGTKERSVIKLPGAGLDAVVAQQFE
                     IGRQVLAKGLVPIIEPEVDIKSPRKAEAEDQLKASLLDGVNQLGDQKVMLKLTLPDTD
                     NLYRELVEHPNVIRVVALSGGYSRHDACEKLARNNGVIASFSRALTEGLTAQQSDEEF
                     NAMLDAAIAEIAKASAT"
     gene            complement(977..1420)
                     /locus_tag="VGP27_03125"
     CDS             complement(977..1420)
                     /locus_tag="VGP27_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7850343.1"
                     /translation="MSGYRTVVVGTDGSDSSLRAVDKAGQIAAGSGAKVIVATAYFPQ
                     SEDQRAADVLKDEGYKMAGNAPIYAILREAKDRAKAAGAEDVEEKAVVGAPVDALVDL
                     AAEVNADLLVVGNVGLSTIAGRLLGSVPANVARRSKTDVLIVHTS"
     gene            1635..2276
                     /locus_tag="VGP27_03130"
     CDS             1635..2276
                     /locus_tag="VGP27_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560386.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEV7850344.1"
                     /translation="MALPGAAEFVPDTHDLTVLSEASQCCKGCDLYRDATQAVFGAGS
                     TEARVMFVGEQPGDQEDKAGRPFVGPAGRLLDKALVAAGIDRERIYVTNAVKHFKFEQ
                     RGQRRIHKTPSRTEVVACRPWLLAEIEAVEPDVVVLLGATAAKALMGSSFRLTAHRGE
                     VLRLPDDAKIDLDVDFGVVATVHPSAILRGPPEDREKAFDALVADLRVVAELG"
     gene            complement(2291..2364)
                     /locus_tag="VGP27_03135"
     tRNA            complement(2291..2364)
                     /locus_tag="VGP27_03135"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2330..2332),aa:Cys,seq:gca)
     gene            complement(2426..3670)
                     /locus_tag="VGP27_03140"
     CDS             complement(2426..3670)
                     /locus_tag="VGP27_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7850345.1"
                     /translation="MSTQDSQRHLYLGTLLQFALRTVIIGFVTVTLLWEPPNRHEWAC
                     FALLAGYAVVVGSWSAWALRPASRMAAAARTRVTLLVLTADVAVLSIISVLTGITSPE
                     VWTSNVISSGLFLIPLIAAAQLDPTISGAMAIPTVSAFFVTSAFTRAANEEPWPSILL
                     NTAVLAALAGGSVAVSLIQRARVEVIEDLAQQRTQLLQDLLGLEKRERQTISERLHDG
                     ALQYVLVARQDMDDVRDGSGEAVGRVDAALVECSGLLRDVVRELHPEVLARSGLKAAV
                     ETLADGIGIRSGLHVEIDARTWPDGLHTDADHVLYGAAREALTNVIKHARAQNVWVEL
                     ELRDGRATLRIADDGIGISQARLAKSVEEGHIGMASTRTKVLAADGRFDVRPTSPGTE
                     VTVCIPLEPVSVGHASEEMAAL"
     gene            3801..4526
                     /locus_tag="VGP27_03145"
     CDS             3801..4526
                     /locus_tag="VGP27_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV7850346.1"
                     /translation="MAADPPFFGGPGQGVRQIVELFVAFGLTALIGLERELQGKSAGI
                     RTQTIVGTAAALILLVSKYGFSDVLVAGTVEVDPSRVAAQIVSGIGFLGAGIIIFRRG
                     SVHGLTTAAAIWESAAIGMAAGAGLLLLAFTVTGMHFLVVVGFMPLARRLSARLSGAT
                     RVHLTYTEGQGVMSQLLQACERRQWQLTELTADSGGEPLGAAPGQSGVVLTLTGNGIL
                     NAPAVLAGIDGVTTIRQLDEDPD"
     gene            complement(4523..5395)
                     /locus_tag="VGP27_03150"
     CDS             complement(4523..5395)
                     /locus_tag="VGP27_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850347.1"
                     /translation="MDAFSAGQLSRALDRLHSTIYFAPAANERFSELGLKHERMQYFA
                     SRSAPMGAVTAKVVAATFYNFNPELVAKAIPAAWEVASPATVTRVRYEIVDAVIPKIL
                     GDERVRSPEFAHSTAIVRRAAEAIPNADGRALYAGHAELDWPELPHAQLWHAITLLRE
                     YRGDGHVAALVTHGLSGLEALITHVATGTGFAPDYGRKLRGWSQQQWDESVERLRSRG
                     LLDDGGQLTAAGNDLRSKIEDLTDDLAYAPWRTVSDDDVQELVSLGAIIRAAVRAAGV
                     FPSNAFGPRYGQHR"
     gene            complement(5406..6803)
                     /locus_tag="VGP27_03155"
     CDS             complement(5406..6803)
                     /locus_tag="VGP27_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850348.1"
                     /translation="MTETATIKPGTWGELLGRERLGTATLLAGGVGLYATNEFLTISL
                     LPSTVADIGGQRFYAWVIAVYLVGSVVAAAAVNPLLVRFGPRASYLFTLAIFAVGTLG
                     CALAPNMELLLVARTVQGAAGGMLAGLGYAVINAALPNWLWTRASALVSAMWGVSVII
                     GPAMGGIFAQLGIWRWAFGSLVIMTCAMAALVPGALPARQQSEEKPSAIPRIPLWSLL
                     LLGAAALAISAAGVQRAAAATFGLLTVGAVLVGLFLVVDRKAKATVLPRAAFGPGPLK
                     WMYVTIGLLMAATMVDMYVPLFAQHLAGMVPAAAGFFGAVLAVGWTLSEVASASVSRT
                     AVIVRLVAVAPVVMAVGLAIGALVLGDHLTWPLISAWAVGLFITGSGVGIAWPHLSAW
                     IMGAIDEPVEAGTAAAAINTVQLISGSFGAGLAGVVVNVTQGGDAAAARWLSIVFAVL
                     AAAGMVASYRASRGR"
     gene            complement(6844..>7941)
                     /locus_tag="VGP27_03160"
     CDS             complement(6844..>7941)
                     /locus_tag="VGP27_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV7850349.1"
                     /translation="DFLLVIDESHVTVPQIGGMYEGDISRKRNLVDFGFRLPSAVDNR
                     PLTWEEFANRIGQTVYLSATPGPYELSQTAGEFVEQVIRPTGLVDPQVVVKPTKGQID
                     DLIGEIRKRTERDERVLVTTLTKKMAEDLTDYLLELGIRVRYLHSEVDTLRRVELLRQ
                     LRLGEYDVLVGINLLREGLDLPEVSLVSILDADKEGFLRSARSLIQTIGRAARNVSGE
                     VHMYADKITDSMKQAIDETDRRRAKQVAYNEEHGIDPQPLRKKIADILDQVYREADDT
                     DVEIGGSGRNASRGRRAQGEPGRAVSAGIVEGRDTSNMPRAELADLIKDLTAQMMAAA
                     RDLQFELAARIRDEIADLKKELRGMDAAGLK"
BASE COUNT         1438 a         2673 c         2497 g         1333 t
ORIGIN      
        1 cgcccgacct tcccgacgat cgcctgtgca aacatgcaca tgaggacgcc ttatacgcgg
       61 cgctgacgtg cggaaggtag tgatgaacac agaacagtac gaaaagatgg gtaaaggcgc
      121 gggcttcatc gccgcgctgg accaaagcgg tggcagcacg ccgaaggcac tcaagctgta
      181 cggcatcgaa gagagcgcgt actcgaacga cgagcagatg ttcgacctgg tgcacgagat
      241 gcgcacccgc atcatcacca gccccgcctt cgacggtgac cgcatcatgg gcgccattct
      301 gttcgagatg acgatggatc gccttatcga cggtcgcccg agcgccgact acctgtggaa
      361 cgtcaagggc atcgtgccgt tcctgaagat cgacaagggc ttggcagatg agcaagacgg
      421 cgcgcagacg atgaagccga tccccggcct tgacgagctg ctggaccgcg cggtcgccaa
      481 cgacgtcttc ggtaccaagg agaggtccgt catcaaactg cccggcgccg gcctggacgc
      541 ggtcgttgcc cagcagttcg agatcggaag gcaggtgctc gcgaagggcc ttgtgccgat
      601 catcgaaccg gaggtcgaca tcaagagccc gcgcaaggcc gaggccgagg atcagctcaa
      661 ggcaagtctt ctcgacggcg tgaaccaact cggtgaccag aaggtcatgc tcaagctcac
      721 tctgccggac accgacaacc tctaccggga actcgtcgag catcccaacg tgattcgtgt
      781 ggtggcactc tccggcggct acagccgcca cgacgcgtgc gagaagctgg cgcgcaacaa
      841 cggcgtgatc gcgagcttct cccgtgcgct gaccgagggg ctgaccgcac agcagagcga
      901 cgaagagttc aacgcgatgc tggatgccgc catcgccgaa attgccaagg cgtcggctac
      961 gtaatctcgg tggggattac gaggtgtgaa cgatcagcac gtcggtcttg gatcgccgcg
     1021 ccacattggc gggcaccgag ccgagcagcc gaccggcgat ggtgctgagc ccgacgttgc
     1081 cgaccacgag taggtcggcg ttgacttctg cggcgaggtc gacgagggcg tccaccggcg
     1141 caccgacgac ggccttctcc tcgacgtctt ccgccccggc ggcctttgcg cggtctttcg
     1201 cctcccgcag gatcgcgtag atcggagcgt tgccggccat cttgtagccc tcgtccttca
     1261 ggacgtcggc ggcccgctgg tcttcgctct gggggaagta cgcggtcgcg acgatcacct
     1321 tcgcacccga ccccgcagcg atctgaccgg ccttgtccac cgcacgcaac gacgaatccg
     1381 agccgtccgt gccgaccaca acggtccgat agccgctcat ccataccctc ccagagtcat
     1441 ttgcaccgcc aacgaacgac cctaacccgt cgtcgggcaa acatcacagt gattcctcgg
     1501 cgtgaacagc attgtcctgg gatttcgtgt tttcggcgca gcgaaggtca cgtcgcacgg
     1561 tggacgtgac agcgacaaca gaaagctgtc gctgcccctg agtggcacgg cgagtagcgg
     1621 gtaatccggt gcatatggca ttgccaggcg ccgcagaatt cgtgcccgac acccacgatt
     1681 tgaccgtcct gtccgaggcg tcgcagtgct gcaaaggctg cgacctctat cgggacgcta
     1741 ctcaagcggt gttcggtgct ggatcgacgg aggccagggt gatgttcgtc ggcgaacagc
     1801 ccggcgatca ggaggacaag gcggggcggc cgttcgtcgg tccggccggt cggctgttgg
     1861 acaaggctct cgtcgccgcc ggtatcgatc gcgaacgcat atatgtcacc aacgcggtca
     1921 agcatttcaa attcgagcag cgcggtcagc gacgcattca caagactccg agccgaaccg
     1981 aggtagtggc atgccgaccc tggttgttgg ccgaaatcga ggcggtagag cccgatgtcg
     2041 tcgttctact cggtgcgacg gcggcgaagg ctctgatggg cagcagcttt cggctgaccg
     2101 cgcaccgcgg cgaggtgctt cgactgccag atgacgcaaa gatcgacctc gatgtcgatt
     2161 tcggagtcgt cgcgaccgta catccgtcag cgatcctgcg cgggccgcca gaagaccgcg
     2221 agaaggcgtt cgatgccctc gtcgccgatc tacgcgtcgt cgccgaattg ggttgacgct
     2281 gagatcttct tggagcgagt gacgggattc gaacccgtgt acacggcttt gcaggccgct
     2341 gcctagccgc tcagccacac ccgcgtcgtg atatgactgt gccgacgggc gccgacacag
     2401 gcaaccattc ggctcgcggt gatcgctaaa gcgcagccat ctcctcggac gcatggccga
     2461 cagaaactgg ttcgagcgga atgcagacgg tcacctcggt tcccggtgac gtcggtctga
     2521 cgtcgaaccg cccgtcggct gcgagtacct tcgtgcgggt cgaggccatg ccgatgtgcc
     2581 cctcttcgac gctcttggcg agccttgcct gcgagatgcc gattccgtcg tctgctatcc
     2641 gcaatgtcgc ccgcccatcg cggagctcca attcgaccca gacattctgt gcccgcgcgt
     2701 gtttgatcac gttggtcaac gcctccctgg ccgccccgta gagcacgtga tcggcatcgg
     2761 tgtgcaaacc atccggccat gtcctagcgt cgatctcgac gtgcagacca ctacggatgc
     2821 cgattccgtc ggccagcgtt tcgacggccg ccttcaaccc ggatcgggca agcacttcag
     2881 ggtgcagttc acggacgacg tcgcgcagca gtcccgaaca ctcgacgagc gccgcgtcca
     2941 ctctgcccac cgcctcacct gaaccgtcgc gcacgtcgtc catgtcctgg cgggccacca
     3001 gcacgtactg cagcgccccg tcgtgcagcc gctcggaaat cgtttgtcgc tcacgctttt
     3061 ccagccccag taagtcctgg agcagttgag ttcgctgctg ggccaagtct tcgatcacct
     3121 ctaccctggc ccgctgaatg agcgacacag cgacggagcc acccgccagg gctgccagaa
     3181 cggccgtgtt cagcagaatc gagggccacg gctcttcgtt cgccgctctg gtgaatgcag
     3241 aagtcacgaa gaatgctgaa accgtcggga tcgccatcgc accgctgatg gtgggatcga
     3301 gttgcgcggc ggcgatcaac gggatcagga acagcccgct gctgatcacg ttggaggtcc
     3361 acacctctgg cgacgtgatc cccgtcagca ccgagatgat ggacaagacc gcgacatccg
     3421 ccgtcagcac cagcagcgtg acgcgagtcc gggctgccgc cgccatccgc gacgccggcc
     3481 tcagcgccca cgcgctccac gagccgacta ccacggcgta ccccgccaac agggcgaagc
     3541 atgcccattc atgccggttc ggcggttccc agagcaaagt cacagtcacg aacccgatga
     3601 tcactgtccg caacgcgaac tgcagcaggg tcccgagata caaatgtcgc tgtgaatcct
     3661 gtgtggacat cgacgactcc gatcgcattg agcctggcgg aagcatagtc ggtagaagcc
     3721 gccagcaaat cgacgcgcgc acggcgcgcc gggactcgac acatagacac cggcactagc
     3781 ttgatcggat ggatctctga ctggcagccg atccgccgtt cttcggcggc cctggacagg
     3841 gcgtccggca gatcgtcgag ttgttcgtcg ccttcgggtt gaccgcgctc atcggactgg
     3901 agcgcgagct tcaaggcaag agcgccggga tccgcacgca gaccatcgtg ggcacagcgg
     3961 cggccctcat tctcctggtg agcaagtacg ggttcagcga cgtgctcgtc gcaggcaccg
     4021 tagaggtcga tccatcccgg gttgcggccc aaatcgtctc cggcatcggc tttctcggcg
     4081 cgggcatcat catcttccgc cgcggttcgg tacatgggct gacgaccgcg gcagccatct
     4141 gggagtccgc cgcgatcgga atggcggccg gcgcaggtct gctcctcctc gctttcacgg
     4201 tgacgggcat gcacttcctc gtggtcgtcg gcttcatgcc actggcccga cgcctgagcg
     4261 cccggctcag tggcgcgacc cgcgtgcacc tcacctatac cgaaggacaa ggcgtcatga
     4321 gtcaactgct gcaggcgtgc gaacggcggc agtggcagct cacggagctg accgcggact
     4381 cgggcggcga accgctcggc gcagccccgg gccagtcggg cgtcgtgttg acgctgacag
     4441 gcaacggcat cctgaacgca ccggcagtgc tggcgggaat cgacggcgtg acgacgatcc
     4501 gtcagctcga cgaggacccg gactagcgat gctgcccgta ccgcgggccg aaggcgttgc
     4561 tcggaaacac gccggcggcg cgaacggcgg cgcgaatgat ggcccctagc gaaaccagtt
     4621 cctggacatc gtcgtcggac accgtccgcc acggcgcgta cgccagatcg tcggtcagat
     4681 cctcgatctt gctgcgcaga tcgttgccgg ccgccgtcag ctgacctccg tcgtcgagca
     4741 gacctcgcga gcgcaacctc tcaaccgatt cgtcccactg ctgttgactc cacccccgca
     4801 gtttccggcc gtagtcggga gcaaacccgg ttcccgtcgc gacatgggtg atcagcgctt
     4861 ccagaccgct gagcccgtgg gtcaccagag cggcgacgtg gccgtcgccc cggtattcgc
     4921 gtagcagtgt gatcgcgtgc cacagctgcg cgtgcggtag ctccggccag tccagctcgg
     4981 cgtggccggc gtagagcgcg cggccgtccg cgttcggaat ggcctcggca gcgcggcgca
     5041 ctattgccgt ggagtgcgcg aattccggtg atcggacccg ctcgtccccg aggatcttcg
     5101 gaatcaccgc atcgacgatt tcgtaacgca cccgggtgac ggttgccggt gatgcgacct
     5161 cccacgctgc cggaatcgcc tttgcgacca gctcggggtt gaagttatag aaggtcgcgg
     5221 caacgacttt cgccgtgacg gctcccatcg gcgccgaccg acttgcgaag tactgcatgc
     5281 gttcgtgctt caggccgagt tcgctgaatc tctcgttggc cgcgggggcg aagtaaatcg
     5341 tcgaatgcaa tctgtcgagg gcacggctca gctgcccggc ggagaacgcg tccattgaat
     5401 cgaccctagc gtccgcgcga tgcgcgatac gaggcgacca tcccggccgc ggcgagcaca
     5461 gcgaacacta tggacaacca gcgggccgcg gccgcgtcac cgccctgggt gacgttgacg
     5521 acgacgcccg ccagtcccgc cccgaacgac ccggaaatca gctgcacggt gttgatcgcg
     5581 gccgcggcgg tgcctgcctc caccggttcg tcgatcgcgc ccatgatcca ggccgacagg
     5641 tgcggccaag cgatgccgac acccgagccg gtgatgaaca gcccgacggc ccacgccgat
     5701 atcaaaggcc acgtcaagtg gtccccgagg accagtgcgc cgattgccag cccgaccgcc
     5761 atgaccacgg gagccacggc gaccaggcgg acgatcacgg ctgtccggct gaccgacgca
     5821 ctcgcaacct cggacaacgt ccagcccacc gccagcactg cgccgaagaa tcccgccgcc
     5881 gcgggcacca tgcccgccag gtgctgggcg aacaggggca cgtacatgtc gaccatcgtc
     5941 gcggccatca gcaggccgat cgtgacatac atccacttca gcggaccggg gccgaatgcg
     6001 gcccgcggca acaccgtcgc cttcgcttta cggtcgacga cgaggaacag tccgaccagc
     6061 accgcgccga cggtgaggag gccgaacgtg gcggcggcgg cccgctgtac gcctgcggcg
     6121 ctgatcgcca gcgcggccgc accgagcagc aacagtgacc acaacggaat ccgtgggatc
     6181 gccgaaggtt tctcttcact ctgctgccgg gcaggcagtg cgcccgggac gagggcagcc
     6241 atcgcacacg tcatgatcac cagcgagccg aaagcccagc gccagatgcc caattgggcg
     6301 aagatgccgc ccatcgcggg gccgatgatc acgctgacac cccacatcgc agacaccaac
     6361 gccgacgccc tcgtccacaa ccagttcggc agcgccgcat tgatcacggc gtaaccgagg
     6421 ccggcgagca ttccgccggc tgcgccttgc acggtgcggg cgaccagcag cagctccata
     6481 ttcggagcaa gcgcacatcc caatgtgccg acggcgaaga tggccagcgt gaagaggtat
     6541 gacgcccgcg gcccgaacct gacgagaagt gggttgacgg cggccgccgc aacgacggag
     6601 ccgacgaggt acaccgcgat cacccatgcg tagaaccgct gaccgccgat gtcggcgaca
     6661 gtgctcggca acaggctgat ggtgaggaac tcgttggtcg cgtagagccc gactccaccg
     6721 gccagcaggg ttgcggtgcc gagccgctca cggcccagca gctctcccca ggtacccggc
     6781 ttgatcgtcg cggtctcagt cacatccgcc accgtatgac ctcaaccaca gttgagatca
     6841 acgttatttc agtccggccg catccatgcc gcgtaattcc ttcttcaaat ccgcgatctc
     6901 gtcgcggatt cgggccgcca gctcgaactg caggtcgcgc gccgccgcca tcatctgcgc
     6961 ggtcagatcc ttgatcaggt cggcgagttc ggccctcggc atgttcgagg tgtcgcgtcc
     7021 ctcgacgatc ccggcgctca cggcccttcc cggctctcct tgggcgcggc ggccccgcga
     7081 cgcattgcgt cccgatcctc cgatctcgac atcggtgtcg tcggcctcgc ggtagacctg
     7141 gtcgaggatg tcggcgatct tcttgcgaag cggctgtgga tcgatgccgt gctcctcgtt
     7201 gtacgcgacc tgcttcgcgc ggcggcggtc cgtctcgtcg atggcctgct tcatcgagtc
     7261 ggtgatcttg tctgcgtaca tgtgcacctc gcccgacacg ttgcgcgcag cacgaccgat
     7321 ggtctggatc aggctgcgcg ccgaccgcag gaatccctcc ttgtcggcgt cgaggatcga
     7381 aaccagcgac acctccggca gatccaggcc ctcgcgcagc aggttgatgc cgacgagtac
     7441 gtcgtactcg ccgagccgca gctgccgaag cagctccacc cggcgcaggg tgtcgacctc
     7501 tgagtgcagg tagcgcaccc ggatgccgag ctccagcagg tagtcggtga gatcctcggc
     7561 catcttcttg gtcagcgtcg tgacgagcac gcgttcgtca cgctcggtcc gcttgcgaat
     7621 ctcgccgatc agatcgtcga tctggccctt cgtcggtttg accaccacct gcgggtcgac
     7681 gagtcccgtc gggcggatga cctgttcgac gaactcgccg gcggtctggc tcagctcgta
     7741 tggtcccggt gtcgccgaca ggtacaccgt ctggccgatc cggttggcga actcctccca
     7801 ggtcagcggc cggttgtcga ccgccgacgg aagcctgaaa ccgaagtcca ccaggttgcg
     7861 cttgcgggag atgtcgccct cgtacatgcc gccgatctgc ggaaccgtga cgtgcgactc
     7921 gtcgatgacg agcaggaaat c
//
LOCUS       DASYLX010000131         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1652035,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000131 DASYLX010000000
VERSION     DASYLX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652035"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /locus_tag="VGP27_03165"
     CDS             complement(<1..2196)
                     /locus_tag="VGP27_03165"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEV7850350.1"
                     /translation="MTEQKPHSAAGASSSGVPAAVSTKAATDLKAACEKLLSGSHHLN
                     AAALRDALLDLHEFWLSTKAAEIGITGTSGFAIVATGGLGRRELLPYSDLDLMLLHDN
                     VGRKRVSEVAEGLWYPLWDANIRLDHSVRTVAEAMKVAAQDISAGLAMLEVRHIAGDS
                     DLSSLLVGGARRQWRTGIASRFDDLVEHTKQRWQRSGQIAHRAEPDLKTGRGGLRDVQ
                     LLNALAIAQLADVYPSRSLASPTGTLGEAHLALLNVRTELHRVSGRGRELVLAQHADE
                     IGAALRIGDRFDLARTLSDAARTISFYVDAGLRTAANALPRRGFAALRRPVRRPLDEG
                     VIEFGGEVILARDARPERDPGLILRVASASGTTGLPMAASTLARLGETAPELRTPWPA
                     QALKDLLVMLAAGPTAVATIEALDRTGLWGRLFPEWGAVRDLPPRDVVHIWTVDRHLV
                     ETVSRASAFTTRVSRPDLLLLGALCHDIGKGRGGDHSVIGAELATQIGTRLGLWPSDV
                     ELLSKVVRHHLLLPHTATRRDLQDPKTIDAVVGALEGDTVLLELLHVLAEADSLATGP
                     GVWGDWKASLIGDLVRRCRLVMAGEELPRPDPIDPHHLSLAATVGVHVEMTPGDSPHI
                     YHLTMIAPDRRGLLSKAAGVLALNSLRVHSASVNGHEGAAINTFVVSPHFGTPPAAEL
                     LRQQLIVALDGELDVLASLDRRDRDAAQHGTTRAGEVLAAVPIAHAAAPP"
     gene            complement(2273..2611)
                     /locus_tag="VGP27_03170"
     CDS             complement(2273..2611)
                     /locus_tag="VGP27_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEV7850351.1"
                     /translation="MKLITAIVKPFTLEDVKTGLEQTGILGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKVRIEVLIDDSAVDKVVDVIVQAARTGKIGDGKVWVSPVDTVVRV
                     RTGERGSDAL"
     gene            complement(2617..3999)
                     /locus_tag="VGP27_03175"
     CDS             complement(2617..3999)
                     /locus_tag="VGP27_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV7850352.1"
                     /translation="MDGFPLMGLPDTGDTAWMLASSALVLLMTPGLAFFYGGMVRAKG
                     VLNMMMMSVSAMGVVTVLWVLYGYSLAFGNDVGNLFGNPAQYFGLKGLIGGNAAEAVV
                     ADPSAGVEAADAVNIPLAGTIPQLVFVAFQLMFAIITVALISGAVADRLKFGSWLVFA
                     GLWATLVYFPVAHWVFAFDGFTGETGGWIANKLKAIDFAGGTAVHINSGAAGLALCLV
                     LGRRKGWPTTPMRPHNLPFVMLGAGLLWFGWYGFNAGSALSSNGTAATTFVTTTTATA
                     AAMLAWLLVEKFRDGRATSLGAASGVVAGLVAITPSCSSVNVVGALVVGALGGAICAL
                     AVGLKFKFGYDDALDVVGVHLVGGLTGTLLIGFLATPQAPAAVTGLFYGGGVDQLWRQ
                     AVGAFAVLAYSFIVTTILAYILKFTIGLRVGEEEEVDGIDEAEHAETGYDLATVGGGS
                     SVLGRGLSEE"
     gene            complement(4174..>4516)
                     /locus_tag="VGP27_03180"
     CDS             complement(4174..>4516)
                     /locus_tag="VGP27_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV7850353.1"
                     /translation="LIDTAGRLHTKTGLMDELGKVKRVVSRRAAVDEVLLVLDATIGQ
                     NGLAQARVFADVVDITGVVLTKLDGTAKGGIVFRVQQELGVPVKLVGLGEGPDDLAPF
                     EPGAFVDALLG"
BASE COUNT          783 a         1539 c         1481 g          713 t
ORIGIN      
        1 cggcggcgcg gcggcgtggg cgatgggcac ggcggcgagc acctcacccg cccgcgtcgt
       61 gccgtgctgc gcggcgtcgc ggtcacgccg gtccaatgac gccagtacgt cgagttcgcc
      121 atccagggcg acgatgagct gctggcgcag cagttcggcc gcgggcggag tgccgaaatg
      181 cggtgagacg acgaaggtgt tgatcgccgc gccctcgtgg ccgttgaccg atgccgaatg
      241 cacccgaagc gagttcaggg ccagcacacc ggcggccttc gacagcagcc cgcgccgatc
      301 cggcgcgatc atcgtcaggt ggtagatgtg cgggctgtcc cccggcgtca tctcgacgtg
      361 cacgcccacc gtcgccgcca gcgaaaggtg gtgcggatca atgggatcgg gacgcggcag
      421 ctcttcgccg gccattacca accggcagcg ccgcaccagg tcgccgatca gcgatgcctt
      481 ccagtccccc cacacaccgg gaccggtggc cagcgagtcg gcctcggcca gcacgtgcag
      541 caactcgagc aggaccgtat cgccttccag cgcgccgacg accgcatcga tggtcttcgg
      601 gtcctgcagg tcgcggcgag ttgcggtgtg cggcaggagc aggtgatgcc gcaccacctt
      661 ggagagcagt tcgacgtccg acggccacag cccgagccgg gtgccgatct gggtggcgag
      721 ttcggccccg atgacgctgt ggtctccacc ccggcccttg ccgatgtcgt ggcagagcgc
      781 accgagcaag agcagatcgg gccgcgagac gcgagtcgtg aaagcgcttg cacgcgaaac
      841 cgtttcgacg agatgccggt ccacggtcca gatgtgcacg acgtcgcgcg gcggcaagtc
      901 gcgcacggcg ccccactccg ggaacagcct gccccacagg ccggttcggt cgagcgcctc
      961 gatggtggcc accgcggtgg gtccggcggc cagcatcacc agcaggtcct tgagtgcctg
     1021 cgccggccac ggggtgcgca gttcgggcgc cgtttcgccg agccgggcca acgttgatgc
     1081 cgccatcggc agaccggtcg tccccgacgc cgaagcaacc cgcaggatga ggccggggtc
     1141 gcgctcggga cgcgcgtcac gggccaggat gacctcgccg ccgaattcga tgacaccttc
     1201 gtccagcggc cggcggaccg ggcgccggag cgcggcgaaa ccgcggcgcg gcagggcgtt
     1261 ggcagcggtg cgcaacccgg cgtcgacgta gaagctgatc gtgcgggcgg cgtcggacag
     1321 cgtgcgggcc agatcgaagc ggtcgccgat ccgcaatgcc gcgccgatct cgtcggcgtg
     1381 ctgggccagc acgagctccc ggccgcggcc cgatacgcgg tgcaactcgg tgcgaacgtt
     1441 gagcagggcc aggtgcgcct caccgagggt gccggttggt gaggccagcg atcggctcgg
     1501 gtacacgtcg gcgagctggg cgatcgccag cgcgttcagc aactgcacat cgcgcagacc
     1561 gccgcgccct gtcttcaggt caggttcggc acggtgggcg atctggccgc tgcgttgcca
     1621 ccgctgcttg gtgtgttcga cgaggtcgtc gaagcgcgag gcgattccgg tgcgccactg
     1681 ccgccgtgct ccacccacca gtagcgacga aaggtcggag tcgcccgcga tatgacgcac
     1741 ctccaacatc gccagccctg ccgagatgtc ctgtgctgca accttcattg cctcggcaac
     1801 tgttcggacg ctgtggtcga gccgaatatt ggcgtcccac aacgggtacc acagaccctc
     1861 ggcgacctcg ctgacgcgct tcctacccac gttgtcgtgc aacagcatca ggtcgagatc
     1921 ggagtacggc aacagttccc ggcgaccgag gcctccggtc gcgacgatgg cgaagccgct
     1981 ggtgccggtg atcccgattt cggccgcctt cgtcgacagc caaaactcgt gcagatcgag
     2041 caaagcgtcg cgcagcgccg cggcattcag gtggtgcgaa cccgacagca gcttctcgca
     2101 tgcggctttg aggtcggtgg cggccttcgt cgagaccgcc gccggaacac ccgatgagga
     2161 ggcgccggcg gcggaatgtg gtttctgctc tgtcatttcg gtgtcctccc ccggcctctt
     2221 accatttaac gctcacagtt caaggccggg agacgacgcc tcacttgact ggctacaggg
     2281 cgtcggaccc ccgctcgccg gtgcggaccc ggaccacggt gtcgaccggg ctcacccaga
     2341 ccttgccgtc gccgatcttt ccggtgcggg cggcctgcac gatcacgtcc accaccttgt
     2401 cgacggcgga gtcgtcgata agcacctcga tgcgcacctt gggcacgaag tcaacggagt
     2461 actccgcgcc gcggtacacc tcggtgtggc ccttctggcg tccgtagcct tgcacctcgc
     2521 tgacggtcat tccgagaatt ccggtctgct cgagtccggt cttgacgtct tcgagcgtga
     2581 acggcttgac gatcgcagta atcagcttca tgttcctcat tcctccgaga gaccgcggcc
     2641 gagaacagag ctaccaccgc cgacagtcgc aaggtcgtat ccggtttcag cgtgttcagc
     2701 ctcgtcgatg ccgtccactt cctcttcttc acccacgcga aggccgatgg tgaatttcag
     2761 gatgtacgcc aagatagtcg taacgatgaa cgagtacgca agaactgcga acgctcccac
     2821 cgcctggcgc cacaactggt cgacgccgcc gccgtagaac agacccgtga cagcggcagg
     2881 cgcctgcggg gtcgcgagga agccgatcag cagggtgccc gtcagaccac cgaccaagtg
     2941 gactccgacc acgtcgaggg cgtcgtcgta gccgaacttg aacttcaacc cgacggcgag
     3001 agcacagatc gcgccgccaa gggcgccgac gacaagcgcg ccgaccacgt tgaccgacga
     3061 gcaggagggc gtgatcgcga cgaggccggc cacgacgcct gaggctgcgc ccagcgaggt
     3121 ggcgcgcccg tcgcggaact tctcgacgag cagccaggcg agcatcgcgg cggcggtcgc
     3181 cgtcgtggtg gtcacgaacg tcgtggccgc ggttccgttc gagctcaagg ccgaacccgc
     3241 gttgaacccg taccagccga accacagcag accggcaccg agcatgacga acggcaggtt
     3301 gtgcggccgc atcggggtgg tgggccaacc cttgcgccga ccgaggacga ggcacagtgc
     3361 cagacccgcg gcgccggagt tgatgtggac cgccgtaccg ccggcgaagt cgatcgcctt
     3421 gagcttgttg gcaatccagc cacctgtctc gcccgtgaac ccgtcgaagg cgaacaccca
     3481 gtgcgcgacg gggaaataga cgagcgtcgc ccacagcccc gcgaagacca gccatgagcc
     3541 gaacttgagg cgatcggcga cggcgcccga gatcaacgcg acggtgatga tggcgaacat
     3601 caactggaac gccacgaaaa caagctgcgg gatggtcccg gccagcggaa tgttcacggc
     3661 gtcggccgcc tcgactcctg ccgagggatc agcaacaacc gcctcggcgg cattgccgcc
     3721 gatcaggccc ttcaggccga agtactgcgc ggggttgccg aacaggttgc cgacgtcgtt
     3781 gccgaaggca agcgagtagc cgtacagcac ccacagcacg gtcacgacgc ccatcgcgct
     3841 gacgctcatc atcatcatgt tcagcacgcc tttggcgcgc accatgccgc cgtagaagaa
     3901 cgccaggcct ggcgtcatca gcagcacgag tgctgagctc gccagcatcc atgccgtatc
     3961 cccggtgtcg gggagaccca ttaacggaaa tccgtccact cccagcttcc tccttcgaca
     4021 aggcagggcc gcaaaggttt tacgaccgtt gacctggcca agacaatgct gagtggttgt
     4081 ttcgtctaag acgccttggc gtttcgctcg tgtgaacgga tacgccggtt acgtttcgcc
     4141 ggtgttacat cgcgcgcttt tgacgtgctt tcgttaaccg agcagggcgt cgacgaacgc
     4201 tcctggctcg aacggggcga gatcgtcggg gccttcgccg agcccgacga gtttgaccgg
     4261 gacacccaat tcctgttgca cgcggaacac gattccgccc ttcgccgtgc cgtccagttt
     4321 ggtcagcacc accccggtga tgtcgacgac gtcagcgaac acgcgcgcct gcgcaagccc
     4381 gttctgcccg atcgtggcgt cgagcaccag cagcacctcg tcgaccgccg cgcgcctgct
     4441 caccacgcgc ttgaccttgc cgagttcgtc catcagccct gtcttggtgt gcaaccgccc
     4501 cgcggtgtcg atcagg
//
LOCUS       DASYLX010000132         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1981092,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000132 DASYLX010000000
VERSION     DASYLX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981092"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VGP27_03185"
     CDS             complement(<1..703)
                     /locus_tag="VGP27_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEV7850354.1"
                     /translation="MIQVCSRCGTRWNVRDRQRVWCPRCNGTLLAPSAQPPSEWSARP
                     TAPAVRPNSQGEQSGPPRLPQGYRWIAVRPGAAPPARRRPRHLGPTPRYAAIPRWGFV
                     EHFDTAEVEQQAEPRRRASVGALRATLVTTIAVLGLAALVHIVRYALLIINRDVLLNP
                     VVAWLASWLGVVVSVAALFLLFATFVLLTNWLIARRSVAFEHVGREEHRPLWTLRAGC
                     LVPFVNLAWAPVFVIE"
     gene            complement(727..1071)
                     /locus_tag="VGP27_03190"
     CDS             complement(727..1071)
                     /locus_tag="VGP27_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7850355.1"
                     /translation="MEGPAGIRQAEMSDVPTTFDESVVLLDVRENDEWQRGHAAGARH
                     IPMGEVPARIGELDPGAELFVVCHLGGRSARVAQYLMQNGYAPINVSGGMQAWADAGR
                     PVVTDGGDAGTV"
     gene            complement(1652..2074)
                     /gene="sodN"
                     /locus_tag="VGP27_03195"
     CDS             complement(1652..2074)
                     /gene="sodN"
                     /locus_tag="VGP27_03195"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEV7850356.1"
                     /translation="MLHRLFSNATEAHAHCDLYCGVYDPAQAKIEALSCLKTLKKFHD
                     SDDDHFKTRAIIIKEQRAEEVKHHLMVLWADFFTKDHFEQFPNLHQLFWDGVHGAGDV
                     KKSTDVAVAEKLLKTIDEISDIFWQTEKAKDMGVYPPA"
     gene            2160..2438
                     /locus_tag="VGP27_03200"
     CDS             2160..2438
                     /locus_tag="VGP27_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEV7850357.1"
                     /translation="MRPALGPGDGLLTARGGRPRRGQLRVFRDPRMSTRWLVKRVGDV
                     YGTGDGALFEARSDNPRAAGAADSHEFGWVPAAGSYRVVLTAKAKVGR"
     gene            2547..3080
                     /locus_tag="VGP27_03205"
     CDS             2547..3080
                     /locus_tag="VGP27_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEV7850358.1"
                     /translation="METGSGRAIEIAPFHSQGALRGFVVSGRWPDSTKEWAQVLRVAV
                     RFASFPGLLATTTIFGAREELPESPQPGTVGLVVAEGTVVGENAVAPGYFAGHQPPAL
                     LMLHPPSETTPSLPECSGAASGCVLLPGLPHLGLEHRAAWVEAESDGTVTSMVSRVGI
                     DPISDPDTAILAMLLAA"
     gene            3523..>3815
                     /locus_tag="VGP27_03210"
     CDS             3523..>3815
                     /locus_tag="VGP27_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7850359.1"
                     /translation="MSKTFSARLNRLFDTVYPPGRGPHTSAEVIGALKSEGITMSAPY
                     LSQLRSGNRTNPSAATMAALANFFRIKPAYFTDDEYYEKLDKELTWLANMRDEG"
BASE COUNT          673 a         1229 c         1201 g          712 t
ORIGIN      
        1 gttcgatgac gaagacgggt gcccacgcca ggttcacgaa cggcacaagg catccggcgc
       61 gcagcgtcca cagtggtcga tgttcctcgc gcccaacatg ttcgaaggcc accgaccgtc
      121 gagcgatcaa ccagttcgtc agaagcacga aggtggcgaa caacaggaac agagcggcca
      181 cgctgaccac gactccgagc cagctggcca accacgccac caccggattc aagagcacgt
      241 cgcggttgat gatcagcagc gcgtatcgca cgatgtgcac gagagcggcg aggccgagca
      301 ccgcgatcgt cgtaaccagg gtggctcgca acgcaccgac cgaggccctg cgccggggtt
      361 cggcctgctg ctcgacttct gcggtgtcga agtgttcgac aaacccccag cgtggaatcg
      421 ccgcatagcg cggcgtcgga cccaggtgtc gcggtcgcct gcgggccggc ggggcagcgc
      481 cgggccggac cgctatccag cggtagccct gcggcaaccg tggcgggccg ctctgttcac
      541 cctggctgtt cggccgtacc gccggcgcgg tcggacgtgc gctccattcc gagggcggct
      601 gcgcggacgg cgccagcaga gtgccgttgc accgcggaca ccagacgcgc tgtcggtccc
      661 gcacattcca ccgcgtcccg cagcgagaac acacctggat catccgacca gcctagcgag
      721 ggttgctcag acggtgcctg cgtcgccgcc atcggtgacg acgggccggc cggcatcggc
      781 ccacgcctgc ataccgccgc tgacattgat cggtgcatac ccgttctgca tcaggtattg
      841 ggccactcgc gcagagcggc caccgagatg gcacaccacg aacagttccg cgccggggtc
      901 gagttcgccg atccgcgcgg gcacctcgcc catcggaatg tgccgggccc cggcggcgtg
      961 gccgcgttgc cactcgtcgt tctcccgaac gtcgagtaag acgaccgact cgtcgaacgt
     1021 cgtcggaacg tcggacatct cggcttgccg gatacctgct gggccttcca tgccctcatg
     1081 ctacgaggcg gccggtcatc gacacacgtc aagcagcaat gtgcggctat ccacagtttc
     1141 cacaggttta tccacaggcc gccgtctgca gaaggcgctt ctgaccttct gaatctgtgt
     1201 gactccttgg cgatcctggg gataacctgg caccgttatc gaacagtgat caacaggcac
     1261 aaaggccccc gagcgaaatg gcttgccgct ctaaccttcc gtcgcacgcg ctctgatttc
     1321 tccatcgtca gccgaagggc tatgatcggc gtcacaacga caattgtgag agatctcacg
     1381 gggtgggtaa ttgaacgcgc aggtcagggg acactaggtg acgacgtcgc atccgttcgg
     1441 acaagggtca gccacgtctt ctgagtggca atcgtccagc cactcgtaca cccgcggtca
     1501 actgatggcc tgcgtccgcg ccggtttgat cgcgctcatt cttctgggcg tcctcgccct
     1561 catcgttctg ttctgacccg tttcgacacc aaacacagaa acggccctgg tctccgcagc
     1621 ggaagagcca gggccgtttt ctttgtctgc ctcaagcggg cgggtagacg cccatgtcct
     1681 tggccttctc ggtctgccag aagatgtcgg agatctcgtc gatcgtcttg agcagcttct
     1741 cggcgacggc aacgtcagtc gacttcttga cgtcacccgc gccgtgcacg ccgtcccaga
     1801 acagctggtg caggttgggg aactgctcga agtggtcctt ggtgaagaag tcggcccaca
     1861 gcaccatcag gtgatgcttg acctcctctg cccgctgctc cttgatgatg atggcgcggg
     1921 tcttgaagtg gtcgtcgtcg gagtcgtgga atttcttgag cgtcttcagg cacgacagcg
     1981 cctcgatctt ggcctgggcc ggatcgtaga caccgcagta caggtcgcag tgagcatggg
     2041 cttcagtggc attggagaaa agtcgatgca gcatagacct aacctaccct tcgagggtgc
     2101 ggcgcaacca tgcacttcgg ctttggccac tgcggacgtt tcgcgtggtc gacgagtcga
     2161 tgcggcccgc gctggggccc ggcgacggct tactgaccgc tcgcggcggc cgtccccggc
     2221 gcggccagtt gcgggtgttc cgtgatccgc gcatgtcgac ccgctggctc gtcaagcgcg
     2281 tcggtgacgt gtacggcacc ggagacggcg cgctattcga agcgcgatcg gacaatccac
     2341 gcgccgcagg tgcggcggac tcccatgaat tcggttgggt gcctgcggcc ggttcgtatc
     2401 gtgtggtgtt gacggcgaag gccaaagtcg gccgttagag cccgtgtcgt cttgcgcgcc
     2461 aggcgttgct gactcatgct gagcggatcg acccagtgga gtgtcggcga tgtccgttca
     2521 ccgaatatca gggtcgcgga ggcgagatgg aaacggggtc cggccgggcg atcgaaatcg
     2581 cccccttcca ttcgcaggga gctctgcggg gattcgtggt gtcaggccga tggccggatt
     2641 ccaccaagga atgggcacag gtattgagag ttgccgtccg gttcgcctcc ttccccggat
     2701 tgctcgccac cacaacaata ttcggcgctc gcgaggagct gccggaatct ccgcagcccg
     2761 gtaccgtcgg cctggttgtg gccgagggca ccgtcgtcgg tgagaatgcg gtggcgccag
     2821 gttatttcgc cggtcatcag ccgccggcgc ttctcatgct ccatccgccg tcggagacca
     2881 ctccgtcgct gccggagtgc tccggtgccg catcgggctg cgtgctgctg cccggccttc
     2941 cccacctggg tctagagcac cgggcggcat gggtggaagc ggagtccgac ggcacggtca
     3001 catcgatggt cagccgcgtc ggcatcgatc cgatcagcga tcccgacacc gcgatcctgg
     3061 ccatgctgct tgccgcctag aggcaggcct cggcgaacgt caccgctgga accgtcgtca
     3121 gaacggacgt cccgtcgggc tggcaattcc ggtacccgtt gcccacccgt acctcgtcgg
     3181 gaggggatcc gtgaaggcgc aaattcagca aacgccggga tccgccagag gcgcggcggc
     3241 gccggttgcc ggtagacgcc atcacgcgca gatagccaat ggtcgggggc ctgcgagcta
     3301 agcacttgct acgtatccgt caaaatttgc ctgaccacat catgagacat cgatgagata
     3361 tggtctaaac gcgctgctcg ccgggatatc gagtcccctg aacaaaaagc cgggttaact
     3421 tttccgcggt ttgcacttgg ttgttggcac aactctcgct acgatgatca gcaaatacgc
     3481 cgcctagcca gtccgctctg tcagcatgtg gaggtcaatt cgatgagcaa gacgttctcc
     3541 gcccgcctaa accgcctgtt cgacacggtt tatccgcccg ggcgtggtcc gcatacgtcg
     3601 gcggaggtga ttggcgccct caagtcggag ggcatcacca tgtcggctcc ttacctttct
     3661 cagctgcgtt caggcaaccg tacgaaccct tcggcggcga cgatggccgc ccttgccaac
     3721 ttcttccgta tcaagccggc ttacttcacc gacgacgagt actacgagaa gctggacaag
     3781 gaactgacgt ggctcgccaa catgcgtgac gaggg
//
LOCUS       DASYLX010000133         7492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3077848,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000133 DASYLX010000000
VERSION     DASYLX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3077848"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2307
                     /locus_tag="VGP27_03215"
     CDS             <1..2307
                     /locus_tag="VGP27_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV7850360.1"
                     /translation="SDILRLLLAAAFLAVVITSSLITRYDWVSLERSISDIVGVLTPT
                     QSNLVYLAYGIAILVLPFGILIGLIGGRHWKLLGAYAAAGVIALVALSVRPGGFAAPR
                     WHWNLADQPKTFLSQFLDDPRWIAMLAAMLTVSGPWLPARWRHWWWALLLAFVPIHLV
                     VSAVVPARTLLGLAVGWFVGALVILVVGTPALEVPLEGAVRAMGRRGCSVTGLTVLRR
                     AGRGSLLLSAACADGATVELELYSPNQRSGGALRQLWRKFTLRGGETAPFHASIRRVV
                     EHRALMAIAIGDLGLANTTPLAVTPLDRGWTLYAHTPARGTPLDDATETTPLPRIWGS
                     LRTLHDQDISHGDLRANKITVDGDTVMFGGFDHSEYGATDTQLQSDIAQLLVTTSALY
                     DTPTAVRAAMGVFGKETILTASRRLTKAAVPARIREPIHNAKAVLGAVREEVRLQTGA
                     DEIETETVTRFNRNQILQMVLLVALVYVALPFISTVPTFLSELRTANWWWALFGLAVS
                     ALKYLGAAAALWACADGLVSFRNLTLMQVANTFAATTTPAGVGGLALSARFLQKGGLG
                     ALRAATAVALQQSVQVVTHITLLILFSAFAGASADLSHFVPSATIVYAVGGVLLVLIA
                     AFVVVPKLRTWLTATVGPRITEVLRDLRELASEPRRLVLIVLGCAATTLGGALALWAS
                     IEAFGGNASFVTVTVVTMIGGTLASAAPTPGGVGAVEAALIGGLAAFGVPAGIAVPSV
                     LLYRVLTCWLPVFVGWFVMRGLSEKDMI"
     gene            2648..3517
                     /locus_tag="VGP27_03220"
     CDS             2648..3517
                     /locus_tag="VGP27_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7850361.1"
                     /translation="MRGRNIAIFLGAVTAASVTPLSEMPAASAQPCPDVEVVFARGTA
                     EEPGAGPTGDAFVNALRSRIGTKTLELYPVDYPATTDFPKALDGVRDASARVESMAAN
                     CPNTNIVLGGFSQGAAVAGFVTANVIPDGAPEGVPNPMPPDIAPHIAAVALFGKPNDR
                     FMRAINQPDVEVGPLYANKTVEMCVDDDFVCSSGRDFNAHTQYVETGKVDDAATFAAA
                     KVTATPPAEAAPQGPAAAGQPHLPTPAAPPLSTIPAAPPPSAAAAQPQQHLQGAPETI
                     YSCTTVCRVVGPA"
     gene            3953..>7492
                     /locus_tag="VGP27_03225"
     CDS             3953..>7492
                     /locus_tag="VGP27_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV7850362.1"
                     /translation="MPEDVRPASPQTLTHVLIRQAQRYGDKVAFAYSCNGEDEPDQLT
                     YRQLDHRARAIASALQRQGAAGERVLILCPSGLDFIAAIFGCFYAGTVAIPVHPPAHS
                     RLISRVASIVADARAAFVVTTAEVEKELKPVVDELPDGASLRWCAVDTDVPDDAAGWV
                     APAIDARSTAFVQYTSGSTGSPKGVHLTHGNLISNLGDIAEILGADDNVRVVSWLPLH
                     HDMGLIGAVFGVLYVGGTCYLMPPSAFIQRPMRWLETISRYRAHFSVAPNFAYELCVE
                     RSSAEERASLDLSSWTSALSGAEPVRVATMQRFTEAFAPAGFRSETFRPVYGLAEATL
                     FVSGAVDRGLAATEMHLDGVALRKNRVVSVAPQHSAAASVVGCGPAADSQEILIADPV
                     TRTPCAPDEVGEIWVGGANVALGYWGKPAATEETFRAHTSDTGRGPFLRTGDLGFVVD
                     GELFVTGRLKDVVIIRGKNYYPNDIEFTVQETHPGLMTGRGAVFSVAPDSGGAEQLIV
                     VQEIDREKITEADTDAVIGAIRTAITAEHEIQPHAVLLVDLLRIPTTSSGKIRRRACK
                     QKFIDGELEAFAQWHAPVNSERPSVAPVENASPVASGRSTQEIEAWFVAQLAAELDLS
                     AAEIDITQPFAYYGLDSVRAIQLMTALDAWLGCKTSPTLAYDYPTIELLAGHLATDTT
                     ADHRAAASEQDRPLSADEPVAIIGIGCRFPGADGPSAFWQMLCDGADAVIDAPKNRWG
                     AGSSAEMTSRSGGFLDHVDQFDAEFFGISPREASRMDPQQRLLLEVAWEALEDAGQVP
                     DELAGSRTGVFTGVSANEYQHLTLSRPDLIDAYSGTGTSMSIAANRLSYIFDFRGPSM
                     SVDTACSSSLVAIHMACRSLRDGESTLALAGGVNVMLTDGPNVNFSKAGVLAADGRCK
                     TFDAAADGWVRGEGAGIVILKPLSRALADGDQVYAVIRGSAMNQDGRTNGLMAPSRRS
                     QEEVLADAYRRAGVSPGTVQYVEIQGLGTLLGDAIEAEALGAVLADGRAPEDPCVIGS
                     VKTNIGHLEGASGVASIIKVALSLRHRIIPPTLNFTKPNPNIPFDTLPLRVAERRLPW
                     PDRGRAIAGVSAFGFGGTNAHVVLAEPPQVRETASPDDTVGGRVEILPLSARSPEALA
                     ELARRYEVALVDGAPLADLCYTAAARRAHHDHRL"
BASE COUNT         1210 a         2498 c         2480 g         1304 t
ORIGIN      
        1 agcgacatcc tccggctgct gctggcggcc gccttccttg cggtcgtcat caccagctcg
       61 ctgatcaccc gatatgactg ggtgtcgctg gagaggtcga tctccgacat cgtcggcgtc
      121 cttactccca cccagtccaa tctggtttac ctcgcctacg gcattgcgat cctggtgctg
      181 ccattcggga tcctgatcgg gctgatcggc ggcaggcatt ggaagctgct gggcgcctac
      241 gcggcggccg gtgtgatcgc gctggtcgcg ttgtcggtcc ggccgggggg cttcgccgcg
      301 ccgcgatggc actggaacct ggccgaccag cccaagacct tcctctcgca attcctcgac
      361 gatccgcgct ggatcgcgat gctcgcggcg atgctcaccg tttcgggtcc ctggctgccc
      421 gcgcggtggc ggcattggtg gtgggcgctg ctgttggcct tcgtgcccat ccacctcgtc
      481 gtcagcgccg tggtgcccgc ccgcacactg ctcggtcttg cggtcggctg gttcgtcggc
      541 gcgctggtga ttctcgtcgt cggcacgccg gcactggagg ttccgctcga gggcgccgtg
      601 cgggcgatgg gccggcgagg ctgctcggtg accgggctga ccgtgcttcg gcgcgccgga
      661 cgcgggtcgc tgctgctttc cgcggcgtgc gccgacggtg ccaccgtgga actcgagttg
      721 tacagcccga accagcgcag cgggggggcg ctgcgccagc tctggcggaa gttcaccctg
      781 cgcggcggcg aaaccgcgcc gttccatgcg tcgattcgcc gggtcgtcga gcaccgcgcg
      841 ttgatggcga tcgcgatcgg tgatctcgga ctggccaaca ccaccccgct cgccgtcacg
      901 ccgctcgatc gagggtggac gctttacgcg cacacgcctg cccgcggcac accgctggac
      961 gatgccaccg agacgacgcc gctgccgcgg atctggggat cgctgcggac ccttcacgat
     1021 caggacatct cccacgggga cctgcgcgcc aacaagatca ccgtcgacgg tgacaccgtg
     1081 atgttcggcg gtttcgacca ctccgagtac ggagccaccg acacccaact gcagtcggac
     1141 atcgcgcagc tgctggtgac gacgagcgcg ctgtacgaca cgccgacggc ggtgcgcgcg
     1201 gcgatgggcg tcttcggcaa ggaaaccatc ctgacggcgt cgcggcgact gacgaaagct
     1261 gctgtgcccg cgcgcattcg agagccgatt cacaatgcca aggcggtgct cggtgctgtg
     1321 cgcgaagagg tgcgactgca gaccggtgcg gatgagatcg agacggagac ggtcacgcgg
     1381 ttcaaccgca atcagattct gcagatggtg ttgctcgtgg cgctggtcta cgtcgcgctg
     1441 ccgttcatca gcacggtgcc gacgtttttg tccgagctga ggacggcgaa ctggtggtgg
     1501 gctctgttcg gcctggcggt ctccgcgttg aagtacctcg gtgcggcggc ggcactgtgg
     1561 gcatgcgccg acgggctggt gagcttccgc aacctcacgc tgatgcaggt ggccaacacc
     1621 ttcgccgcca ccacgacgcc cgcaggagtg ggcggactgg cgttgagcgc acgattcctg
     1681 cagaagggcg gactgggcgc gctgcgggct gccaccgccg tggcactgca gcagtcggtg
     1741 caggtggtca cccacatcac gctgctgatc ctgttcagcg cgttcgccgg agcatcggcc
     1801 gatctgtccc acttcgttcc ctcggcgacc atcgtgtacg ccgtcggcgg tgtactgctg
     1861 gtgctgatcg cggcgttcgt cgtcgtgccc aagctgcgga cgtggctgac ggcaacggtc
     1921 ggaccccgca tcacggaggt cctgcgcgac ctgcgcgagc tggcaagtga gccgcggcgc
     1981 ctggtgctca tcgtgctggg ttgcgcggca acgactctcg gcggagcact ggcgctgtgg
     2041 gcatccatcg aagcgttcgg cggcaacgcc tccttcgtca cggtgaccgt ggtgacgatg
     2101 atcggcggca ccctcgcatc ggcggcaccc actcctggcg gcgtcggtgc ggtggaagca
     2161 gcgttgatcg gcgggctggc agccttcggg gtgcccgccg gaatcgccgt gccgtcagtg
     2221 ctgctctacc gggtgctgac atgctggctg ccggtgttcg tcggctggtt cgtgatgcgt
     2281 gggctgtccg agaaggacat gatctagatg gcggcgctcg ccgagggctc gcgcagtgcg
     2341 cgggagaagc ggcgcccgtc atcggaaaag tggttgtcaa agccggcgaa acagcggccc
     2401 aattcgcatc gtgtgcacgc tcgagggatg tgcggagtgt tgcaattgcg ggcgccgacg
     2461 aaattgcccc gttgcgggat tatttcgctg cagtgcaggc agggccggtg cgactgggcc
     2521 gaaagtcgtg gtgaggaatt cacaccaccg ccgaatgact aagctatcgc tggccgattc
     2581 ataagacgtt cattagctgt tcaattttcg aatcgcgggc gccgtcagtg tgtgtcagca
     2641 ttgcgccgtg aggggacgca atatcgcgat attcctcggt gcggtgacgg cagcgagcgt
     2701 gactccgctg agtgaaatgc cggccgcatc cgcgcaaccg tgcccggacg tggaagtggt
     2761 gttcgcgcgc ggtaccgccg aggaacccgg cgccggaccc accggcgatg ccttcgtcaa
     2821 cgcgctgcgt tctcgcatcg gcacgaagac gctcgagctg tatccggtcg actaccccgc
     2881 caccaccgac ttccccaaag ccctcgacgg cgtccgcgat gccagcgccc gcgtcgagtc
     2941 catggcggcg aactgtccca acaccaacat cgtgctcggc gggttctcac aaggcgccgc
     3001 cgtggccggg ttcgtgactg cgaacgtgat cccggacgga gcaccggagg gcgtgccgaa
     3061 cccgatgccg cccgacatcg ccccgcacat cgccgccgtc gcactgttcg gaaagcccaa
     3121 cgaccggttc atgagggcga tcaatcagcc ggacgtcgag gtgggaccgc tgtacgcgaa
     3181 caagaccgtc gagatgtgtg tcgatgatga cttcgtctgt tcgagcggcc gagacttcaa
     3241 cgcgcacacg cagtatgtgg agaccgggaa ggtggacgac gcggcgacgt tcgccgccgc
     3301 gaaggtgacg gcgacgcccc cggccgaggc ggcgccgcag gggcctgccg cagccggcca
     3361 gccgcatctt ccgacgcccg ccgcaccgcc gctgtcgacg attccggctg caccgccgcc
     3421 gtcggccgcc gccgcgcaac cccaacagca tctgcagggc gcacccgaga cgatctactc
     3481 gtgtacgacg gtgtgccgcg tcgtcgggcc cgcttgaacc atccgcgcct ctagtacgta
     3541 gtacctaggt ccgacccact aaggccgtca ccttccgacg gcaaggaccg catcggcgct
     3601 tgcgcatcca tccggccgca gcgtcgtcgc tgatttgcgc agcaatggag gtagcgacca
     3661 tcaagaccat tgccacatat gccatctgaa gacatcccgg cggaatctga cagtccggtt
     3721 ggaatgtcct gaaagtctaa cgagaccgtc aataacccgg tatgaatgtg gcggacctta
     3781 gcaacgaaac ggtgatcttg gacgcgaacg ctagttcgct gcaacagggc gtgcttaccc
     3841 gcgttgagcc tgtcagcagc gcatcttcgc cttctacaca agggcggcgt accagcgacg
     3901 gcagtggccg cgatcctcag agacactctc agacaacggg tgggagattg atatgccaga
     3961 agacgtgcgg cccgcgtcac cgcaaacgct gacacacgtt ctgattcggc aagcccagcg
     4021 ctacggcgac aaggtcgcgt ttgcctattc gtgcaatggc gaagatgagc ccgaccagct
     4081 gacttaccgg cagttggacc accgggcacg agcaatcgct tccgctttgc agcggcaagg
     4141 tgccgcgggg gaacgtgttc taattctgtg cccgtccggc ttggacttca tcgccgccat
     4201 cttcgggtgc ttctacgcgg gcacggtcgc gattcccgtg catccgccgg cacacagtcg
     4261 actgatctcc cgagtcgcct cgatcgtcgc cgacgcgaga gccgcgttcg tggtgaccac
     4321 tgccgaggtg gaaaaagaac tcaaacctgt tgtcgacgag ttgcccgacg gcgcttcctt
     4381 gcgatggtgt gccgtcgata ccgatgtgcc cgatgatgct gccggctggg tcgcccctgc
     4441 catcgatgct cgatccacgg cgttcgtgca gtacacctcc ggctcgacgg gctcgccgaa
     4501 gggcgtccac ctgacgcacg ggaacctgat ttccaacctg ggcgacatcg ccgagatcct
     4561 gggggccgac gacaacgtgc gtgtcgtgag ctggctgccg cttcaccacg acatgggcct
     4621 catcggggcg gtcttcgggg tcctctacgt cgggggcacc tgttacctca tgccgccgtc
     4681 ggccttcatt cagcggccga tgcgttggct cgagaccatt tcgcgctacc gggcgcactt
     4741 cagcgtcgcc ccgaacttcg cctatgaact ctgtgtcgaa cgaagctcgg cggaggagcg
     4801 ggcgtcgctc gatctctcct catggacctc ggcgctgagc ggcgcagaac ccgtccgggt
     4861 cgcgaccatg caacggttca ctgaagcgtt cgcaccggcg ggattccgtt ctgagacgtt
     4921 ccgtccggtg tacgggctgg cagaggcaac gctttttgtt tcgggcgcgg tcgatcgcgg
     4981 ccttgcggcg accgagatgc acctggacgg cgtcgcgctg cggaagaacc gcgtcgtatc
     5041 ggttgcgccc cagcactcag ccgcggcttc ggtggtgggc tgcggccccg cggccgacag
     5101 ccaggagatc ctcatcgcgg acccggtcac tcgcacacca tgcgcgccgg acgaggtcgg
     5161 cgagatctgg gtcggcggcg ccaatgtcgc gctcggatac tggggcaaac cggccgcgac
     5221 ggaagagaca ttccgggctc atacctcaga caccggacgc ggcccgttcc tgcgcaccgg
     5281 agacctgggc ttcgtcgtcg acggtgagct gttcgtcacg ggacggctca aggacgtggt
     5341 gatcatccgc ggcaagaact attaccccaa cgacatcgaa ttcacggtcc aagaaaccca
     5401 tccgggtctg atgaccggcc gcggcgccgt cttctcggtc gctcccgact ccggcggcgc
     5461 agagcagctc atcgtggtgc aggagatcga ccgcgaaaag atcaccgagg ccgataccga
     5521 tgcagtgatc ggcgccatcc gcacggcgat caccgccgag cacgagatcc agccgcacgc
     5581 ggtcctgctg gtcgatctgc tgcgcatccc gacgacgtcg agcgggaaga tccgcaggcg
     5641 cgcctgcaag cagaagttca tcgacggcga actcgaggcg ttcgcgcagt ggcacgcgcc
     5701 cgtcaatagc gagaggccgt ccgtggcgcc cgtcgagaac gcttctcccg tcgcttctgg
     5761 gcgcagcacc caggagatcg aggcctggtt cgtcgcgcag ctcgccgccg agctcgacct
     5821 gtcggccgcg gagatcgaca tcacccaacc gttcgcttac tacggtctcg actcggtgcg
     5881 ggccattcag ctgatgaccg cgctggacgc gtggctggga tgcaagacgt cgcccaccct
     5941 tgcctacgac tacccgacga tcgagctgct cgccgggcac ttggccaccg ataccaccgc
     6001 ggaccaccgc gctgccgcaa gcgagcaaga ccggcccctc agcgctgacg aacccgtcgc
     6061 gatcatcggc atcggttgcc gctttccggg cgccgacgga ccatcggctt tctggcagat
     6121 gctttgtgac ggcgccgacg cagtgatcga tgcgcccaaa aaccgttggg gcgcagggtc
     6181 gtcagcggaa atgaccagtc ggtcgggcgg cttcctcgat cacgtcgacc agttcgatgc
     6241 ggagttcttc ggcatctccc ctcgcgaggc ctcgcgaatg gatccgcaac agcgccttct
     6301 gctcgaggtg gcgtgggagg cgctcgaaga cgccgggcag gtgcccgacg aactcgcagg
     6361 aagccgcaca ggcgtgttca ccggcgtctc cgccaacgag taccagcacc tcacgctgag
     6421 ccgaccagac ctcatcgacg cctacagcgg caccggtacc tcgatgagca tcgccgccaa
     6481 tcggctctcc tacatcttcg atttccgcgg accgagcatg tcggtcgaca cggcttgttc
     6541 atcgtcattg gtcgccatcc acatggcgtg ccgcagtctg cgtgacggcg agtcgacgct
     6601 ggcgctcgcg ggcggcgtga acgtgatgct gactgacgga cccaacgtca acttctccaa
     6661 ggcaggcgtg ttggcggccg acggccgctg caagaccttc gacgccgcgg ccgacggctg
     6721 ggtccgtgga gagggcgcag gcatcgtcat cctcaagccg ctgagtcgcg ctctggccga
     6781 cggcgaccag gtctacgccg tcatccgggg cagcgccatg aaccaggatg ggcggaccaa
     6841 cggcttgatg gcgcccagca ggcgatctca ggaggaggtg ctggcggacg cttaccggcg
     6901 ggcaggtgtt tcgcccggca cagtccagta cgttgagatc caaggcctcg gcacgctgct
     6961 gggcgacgcg atcgaagcgg aagccctcgg cgcggtgctt gccgacggcc gggccccgga
     7021 ggatccctgc gtcatcgggt cggtcaaaac gaacatcggc catctagagg gtgcgtccgg
     7081 tgtcgccagc atcatcaaag tcgcgttgtc cctccgccat cggatcatcc cgccgaccct
     7141 caatttcacg aagcccaacc cgaacatccc gttcgacacg ctgccgttgc gggtggccga
     7201 gcgccggctg ccttggccgg atcgcggccg ggccattgcg ggagtcagcg cgttcggctt
     7261 cggtggcacc aatgcgcacg tcgtgctggc ggagccgccg caggttcggg agacggcttc
     7321 ccccgacgac accgttggcg gtcgcgttga aatcctgccg ctgtccgcgc gctcaccgga
     7381 ggccctcgcc gagctcgcgc gtcgttatga ggtggctctc gtcgacggcg ctccgcttgc
     7441 cgacctgtgc tacacggcgg ctgcccggcg cgcacaccac gaccatcggt tg
//
LOCUS       DASYLX010000134         7057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3077992,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000134 DASYLX010000000
VERSION     DASYLX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3077992"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGP27_03230"
     CDS             <1..198
                     /locus_tag="VGP27_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7850363.1"
                     /translation="SVYDDLRRHFTEDQLVALGLHCALCVGLGRLAATWHVVDDLPDE
                     YRADTGTPLAPWGANSVVASG"
     gene            576..1502
                     /locus_tag="VGP27_03235"
     CDS             576..1502
                     /locus_tag="VGP27_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7850364.1"
                     /translation="MLALTVSVVQAQTAAAYSRQGLPVEYLEVPSAAMGRDVKIEFQG
                     GGPHAVYLLDGLRATDDASGWDINTSAFEWFYQSGISVVMPVGGQSSFYSDWYQPAAG
                     SSGTQTYKWETFLTQELPMYLQANKGQDPSNNAVVGLSMSGGAAMTLAIWHPAQFIFA
                     GSLSGFLNPSQGLWPTLIGLSMKDAGGFNAVDMWGTTSDPAWRRNDPMVNINTLVANR
                     TALWVYCGNGGLSDLDANDGNFGTQFSAQYLETITVDTNKQFEQKYIAAGGRNAIFKF
                     PENGTHSWGYWGAQLQEMKPDLLRVLGVQQQA"
     gene            complement(1535..2287)
                     /locus_tag="VGP27_03240"
     CDS             complement(1535..2287)
                     /locus_tag="VGP27_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7850365.1"
                     /translation="MKRVRTAALLLAVTVSAAGCSGEIGETLRRLAHPATPSAEAPAE
                     SSDDVVAKARPSVVKVHGESESCFKVSEGSGFVVAPHKVMTNAHVVAGAETFSVDAQA
                     KSLDAQVVSYDPRADIAILDVPGLSAAPLEFAEYTAGTGTAALVLGFPGAASFKASPT
                     QIRQVSDIKGPDIYQATSVTRQVYILIGSFPRPGSSGSAVVDLYGHVLGVYFGAETND
                     STTGFAMTAAQVAPQMAQVGGTQAADTGDCIY"
     gene            complement(2301..3320)
                     /locus_tag="VGP27_03245"
     CDS             complement(2301..3320)
                     /locus_tag="VGP27_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV7850366.1"
                     /translation="MADLMNRQILLRRRPVGLVKPDDTELVTSQAPEPADGEALLRTT
                     YVGIDAAARTWLDDQPGYLPPVQIGEVIRAAGIGEVVASRCDAYGVGDVVTTLTGFQD
                     YVIIRDDIFATPVVGYTDQLAVMSVYGPTGATAYFGMTGVGRPQPAETVVVSAAAGAT
                     GSVAGQIARIAGARVVGIAGGPEKCRAVVDDFGFDACIDYKTEDLRSALKQHCPRGVN
                     VYFDNVGGPILDAVLGRLAPKARVVLCGVISSYLTGDHPGPANYVNLLSKTALMQGFN
                     ALDEWGRFDEAFTALSGWEQEGLLVHRETVFDGLDSCVDALNGLFTGANIGKMLVKVS
                     EPTLT"
     gene            3501..5231
                     /locus_tag="VGP27_03250"
     CDS             3501..5231
                     /locus_tag="VGP27_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HEV7850367.1"
                     /translation="MRRLFYSAGVVALSIASTVVLASMQTMTVLVALTATALIMGGTG
                     HPLSSPQDSPDFINSYTTDANNDYIVLTGFCGAESCTPTTVSTPEQFMPVSGVMPFDQ
                     SVGQGVVNLDQAINAQPVGESMVVFGYSQSARIASIQKANLAASGSTLPVSFVLIGNP
                     NRPNGGILERFEGLKVPIAGITFDGATPTDTNFKTVDVTRQYDGWSDFPNNPLNPFAT
                     ANAIAGIQYLHGDYQSVGLGDALYQGAYGDTDYYMIPSRRLPLLMPLADAGVPSPVVT
                     MLDAPTRVLVEAGYDRTISPGSPTQASILYFPNPMQTGMNFIIAIPTGMDDGAQEAAN
                     IRPFGTPPIDERSPYGVGGPPVNAGSFDSTGAPLVSQPTAPAPDTQPLAPSPTAASIT
                     APPPVIDKAAPATPVTAPTPGWWTPAALPKPAAADVSAPAPAVDPKPAPLDMPKPAPV
                     DLPEPGPLELPKPAPVELPKGDPHTGDLPRVDILPNIVPPKIDPPAQAPIAEPPKPAD
                     VPAAAFVPPAIPAAPLAPPAQAPALPELPVIPAAPPPQLPALPPMPALPPPPPLPNIA
                     GILGGIRLPF"
     gene            complement(5245..5769)
                     /locus_tag="VGP27_03255"
     CDS             complement(5245..5769)
                     /locus_tag="VGP27_03255"
                     /inference="COORDINATES: protein motif:HMM:NF023502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 family protein"
                     /protein_id="HEV7850368.1"
                     /translation="MTRRLGTLIILILALAGCGRDASPPAAQSTSSATEATSSAVAQN
                     PQGEGGSFPDPCTLLSDAEVTELTGRDITQIDRDGADATASTRYCQWQQSSGQLAVFL
                     SRTTSGDFTAAQADSATIPGFGDGAFSRDGHLYVLVGTTQVDVYARGGDEQQSQAEAE
                     KVAGALLPKVRAFS"
     gene            6327..>7057
                     /locus_tag="VGP27_03260"
     CDS             6327..>7057
                     /locus_tag="VGP27_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV7850369.1"
                     /translation="MHKNTVHADATKAAAVEPDFVVLGTVDVERGAIADLAISADGTT
                     IVATHYGDRSVSVVDAYAMTVEADIEMPVEPHLVTATQRRAYVTISGHHYDSIAVIDT
                     ENKQVIANHQVPGVATDVAVSPDATRIFVGRVGDDSVGLAVINTFGRTHILDIADGLG
                     RTVDAVRVSPNGRIVYVATSDADSGTLFLVDAAAGRMIREVPIASPIRDIVLSRDGAL
                     AYVASFDTHWGGSVDIIDTAINEITA"
BASE COUNT         1258 a         2353 c         2217 g         1229 t
ORIGIN      
        1 tcggtgtatg acgacctgcg ccggcatttc actgaggacc agttggtcgc cttgggcctt
       61 cactgcgcgc tgtgcgtcgg gcttgggcgc ctcgccgcga catggcatgt cgtcgacgat
      121 ctgcccgacg aatatcgggc cgacaccggg accccgctcg cgccgtgggg cgcgaattcc
      181 gttgtcgcgt cgggctgaat ctttaccttg aaggcgtctg ccaaggcgtc tgccgctgaa
      241 ccggtgcgag gtgatcgacg tacgcaatcc gcgggagcgt acggaacttg tgcgtgtcgg
      301 cggccgcgga gaccgcgact tcgtcccggt agtcgtcgcg cggaccatca cggtgtccct
      361 cgactgttca cgtcgataga cctcacaatc tcggaacgtg ttgataacaa gccgatcacg
      421 cgcggcgctg cagcgccgcg tctgccatgt gccgttgcta ccttgcgcga aggcgagccc
      481 gagacagatg aggtgtggcg gtgttggtgg cggaacacgt ggtcgacagg acattacgtg
      541 cgtggtcata tgcggtcgcg gtggtatcgg tggcgatgct ggctctgacg gtcagtgtgg
      601 ttcaggctca gaccgctgcg gcctactcgc ggcagggtct gccggttgag tatctggagg
      661 ttccgtcggc tgccatgggg cgcgacgtca agatcgagtt ccagggcggt ggcccgcacg
      721 ccgtgtacct gctggacggt ttacgggcca ccgatgacgc gagtggctgg gacatcaaca
      781 cctcggcgtt cgaatggttc taccagtcgg ggatttcggt agtcatgccc gtcggcggtc
      841 agtccagttt ctactccgac tggtaccagc cggcagcagg tagctcgggc acgcagacat
      901 acaagtggga gacgttcctg acacaggaat tgcccatgta ccttcaggcc aacaagggtc
      961 aggacccgtc caacaatgcc gttgtgggac tgtcgatgtc gggcggtgcg gccatgacgc
     1021 tcgccatctg gcatccggcg cagttcatct tcgccggctc gttgtcgggc ttcctcaacc
     1081 cgtcacaggg tttgtggccg acgctcatcg gcctctcgat gaaggacgcc ggcggcttca
     1141 acgccgtgga catgtgggga accaccagcg acccggcgtg gcgccgcaac gatccgatgg
     1201 tcaacatcaa cacgttggtg gccaaccgaa cggctctctg ggtctactgc ggcaacggcg
     1261 gcctctctga cctcgacgcc aatgacggaa acttcggcac ccagttcagc gcgcagtatc
     1321 tggagaccat cacggtggac accaacaagc agttcgagca gaagtacatc gctgcgggcg
     1381 gacgcaacgc gatcttcaag ttccccgaga acggcaccca cagctggggc tactgggggg
     1441 cgcagctgca ggagatgaag ccggacctgc tgcgtgtgct cggcgtgcaa cagcaggcgt
     1501 gaccggtcac gtccccacac gtagtggagc cgtgctagta aatgcaatcg ccggtgtcgg
     1561 cggcttgcgt accaccgacc tgtgccatct ggggcgccac ctgtgcagca gtcatcgcga
     1621 agccggtcgt cgaatcattg gtctccgcac cgaaatacac gccgagaacg tgcccgtaca
     1681 agtcgacgac ggcgctaccg ctcgagcccg gccgcgggaa cgagccgatc agtatgtaga
     1741 cctgtcgagt gaccgacgtc gcctggtaga tgtctgggcc cttgatatcg gagacctggc
     1801 ggatctgggt gggggaggcc ttgaacgacg cggcaccggg aaagccgagc accagcgcgg
     1861 cagtaccggt gccggccgtg tactcggcga actccaacgg cgccgcagac agaccgggca
     1921 cgtcgaggat tgcgatgtcg gctcgcgggt cataggaaac cacctgcgcg tcaagggact
     1981 tcgcctgcgc gtccacggag aacgtttcgg cgcctgcgac gacgtgagca ttggtcatca
     2041 ccttgtgggg tgcgaccacg aagccgctgc cctcggagac cttgaagcag ctctccgact
     2101 ccccgtgtac tttcaccacg ctgggccgcg ccttggccac gacatcgtcg gacgactctg
     2161 ccggagcttc cgccgatggc gtggccgggt gtgccagccg ccgcagggtc tcgccgatct
     2221 cgccgctgca acccgccgcc gaaaccgtca ccgccagcag caacgccgct gtccgaaccc
     2281 gtttcaccgt cgcgtttcct ctaggtgagc gtcggttcgc tgaccttcac gagcatcttt
     2341 ccgatgttag ccccggtgaa cagcccgttg agcgcgtcga cgcacgagtc gagcccgtcg
     2401 aacaccgtct cgcgatggac gagcaggccc tcctgctccc agccgctcag tgcggtgaag
     2461 gcttcgtcga aacggcccca ctcgtcgagc gcgttgaacc cctgcatcag cgcggttttc
     2521 gacagcaggt tcacgtagtt ggccggtccg ggatggtctc cggtcagata actggaaatg
     2581 acgccgcaca gcacgacccg cgccttcggc gccaagcggc ccaacaccgc atcgaggatc
     2641 ggcccgccga cgttgtcgaa gtagacgttg acgcctcgcg ggcagtgttg cttgagcgcg
     2701 gaccgcaagt cctcggtctt gtagtcgatg caggcgtcga atccgaagtc gtcgaccacc
     2761 gcccggcact tctcgggacc accggcgatg ccgacgacgc gcgcaccggc gatccgcgcg
     2821 atctggccgg cgaccgaccc cgttgcgccg gctgcggccg aaacgacgac cgtctccgcg
     2881 ggctgcggtc gaccgacgcc ggtcatcccg aaataggctg tggcgccggt ggggccgtac
     2941 accgacatga ctgcgagctg gtcggtgtac ccgaccaccg gcgtggcgaa tatgtcatcg
     3001 cggatgatca cgtagtcctg gaaaccggtc agcgtggtga ccacgtcgcc gacgccgtag
     3061 gcgtcgcaac gcgatgccac cacctctccg atgcctgctg cgcggatcac ctctccgatt
     3121 tgcaccggcg gcaggtagcc gggctggtcg tcgagccatg tccgcgccgc tgcgtcgatg
     3181 ccgacatagg tggtgcgaag cagggcctcg ccgtccgccg gctccggtgc ctgcgaggtc
     3241 accagttcgg tgtcgtcggg cttgaccagt ccgacggggc gtcgccggag cagaatctgg
     3301 cggttcatca ggtcggccac gacgccgaaa ctactgaaag cggcggtcgt ccggagcatc
     3361 tacggtgcaa cgtgtccggc tgtgactttg cgcaatcggt tgcgggcaaa ccaatcgagt
     3421 cgcagaattc acttgagcca catgggagcg gctcggcgcg cgggggcgtc caactgcaat
     3481 aactgcttct ggagcttgcc gtgcgccgtc tcttctactc cgccggtgtc gtcgcgctgt
     3541 ctatcgcctc cacggtcgtg ctggcgagca tgcagacgat gactgtgctg gttgcgctca
     3601 ctgcgacggc gctgatcatg ggcggcaccg ggcatccgct gagcagcccg caggacagcc
     3661 cggacttcat caacagctac accaccgacg ccaacaacga ctacatcgtg ctgaccgggt
     3721 tctgcggcgc cgagtcctgt acgccaacaa cggtcagcac acccgagcag ttcatgccgg
     3781 tgtcaggcgt gatgcccttc gaccagtcgg tcggccaagg tgtggtcaat ctcgaccagg
     3841 cgatcaacgc gcaacccgtc ggcgagtcga tggtcgtctt cggttactcg cagagcgcgc
     3901 gaatcgcgtc gatacagaaa gccaacctcg ccgcgtcagg ctcgacactg ccggtgtcgt
     3961 tcgtgttgat cggcaacccg aaccgtccca acggtggcat cctcgagcgc ttcgaaggtc
     4021 tcaaagtgcc cattgccgga atcactttcg acggcgccac acccaccgac acgaacttca
     4081 agaccgtcga cgtcacgcgt cagtacgacg gctggtcgga cttccccaac aacccgctca
     4141 acccgttcgc gaccgccaat gccattgcgg gaattcaata tctgcacggc gactatcaga
     4201 gtgtcggact cggcgatgcg ctgtatcagg gcgcatacgg cgacaccgac tactacatga
     4261 tcccgagccg gcggttgccg ttgctgatgc ccctcgccga tgcgggcgtg ccgagcccgg
     4321 tggtgacgat gctcgacgca ccgacgcggg tgctggtgga agccggctac gaccggacga
     4381 tcagtcccgg ctcgccgaca caggcgagca tcctctactt cccgaatccg atgcagaccg
     4441 gcatgaactt catcatcgct atcccgaccg gaatggatga cggcgcacag gaggccgcca
     4501 acatccgacc gttcggaacg ccaccgatag atgagcgcag cccctacgga gtgggcgggc
     4561 cgcccgtcaa cgccggttca ttcgactcca ccggtgcacc gttggtttcg caaccgaccg
     4621 ctcccgcgcc ggacacgcag ccgctggcgc cttccccgac cgctgcgtcg atcacggcac
     4681 ccccaccggt cattgacaag gccgcacccg cgacaccggt cactgcgccg acgccaggtt
     4741 ggtggacgcc ggctgcgctg ccgaagcccg cggccgccga cgtgtccgcg cccgctccag
     4801 ccgtcgaccc caaaccggcg ccgctcgaca tgccgaaacc ggcacccgtc gacctgcccg
     4861 agcccgggcc gctcgagctt cccaagccgg cacccgtcga gcttcccaag ggcgacccgc
     4921 acacgggtga tctgccgcgg gtggacatcc tgccgaacat cgtgccgccc aagatcgatc
     4981 cgcctgcgca ggcaccgatc gcggaaccgc cgaagccggc ggatgtgccg gccgcggcgt
     5041 tcgtgccgcc cgccatccca gccgcgccgc tggctccgcc tgcgcaggcg ccggcgttac
     5101 cggagctgcc cgtaatcccc gcggcgccgc ctcctcaact gcccgcgctg ccgccgatgc
     5161 cggcattgcc accgccgcca cccctgccga acatcgccgg gatcctcgga gggattcgcc
     5221 tgccgttctg agctgaaaga ccggtcagga gaaggcgcgc accttcggaa gaagtgcccc
     5281 cgctaccttt tcggcttcgg cctgactttg ttgctcgtcg cccccgcgcg cgtagacgtc
     5341 gacctgggtt gtgccgacga gcacgtagag atggccgtcc cgcgagaatg cgccgtcacc
     5401 gaacccggga atcgtcgccg agtctgcctg ggcggcagtg aaatcgccgc ttgtcgtccg
     5461 ggacaggaac actgcgagct ggcccgagga ctgctgccac tggcagtagc gagtcgaggc
     5521 ggtcgcgtcg gcgccgtccc tgtcgatctg agtgatgtca cgccccgtca attcggtgac
     5581 ctcggcgtca ctcaaaaggg tgcacgggtc ggggaagctg ccgccttctc cctgcggatt
     5641 ctgcgcgacg gccgaggacg tcgcctccgt cgccgaagac gtcgattgcg cggcaggcgg
     5701 cgacgcgtca cggccgcaac cggccaaggc cagtatcagg atgatgaggg tgccgagtcg
     5761 gcgagtcatg agttcctctc ggtaacggcg gtccgcgaat cagatacttc accgctgaac
     5821 ggtcggtcgt gccccaatca ggagaatcgg gcagcgtcgg tcgcattcgg atccagcgaa
     5881 cgctcactcg acggcatcgc ggccgccatg gctggttcgg ctgacgcatt cggatgcaac
     5941 acgttcaccg atgggatgcg acacgagttt gctggaacga cattatgtgt tctgcctctc
     6001 actttgatgg ccaaaagtaa gaattcgtta cccaacctca ttggtcacat ttgggcgcgt
     6061 cgcttgctga agacttttat ccccagctca agcccataaa tggagttgcc gaaaaggggt
     6121 aacaactcga taacgaatta tttcctaagc actacctgag acttaactaa agcttgacca
     6181 ttatcggtgt acgacgcttc gtggcgcccg aattttcgtc gctacactcg atcgggacct
     6241 gcgttgaaca agcgcgcaag acgtacatca ccgccggagg aagccacagc cggccacaag
     6301 cactgatgaa cagggcaaat accgtcatgc ataagaacac tgtccacgcg gatgcaacca
     6361 aggccgcggc tgtcgaaccc gacttcgtcg tactggggac cgtcgacgtc gagcgcggtg
     6421 ccatcgccga tctggcgatc agtgccgacg gcaccacaat cgttgcgacc cactacggcg
     6481 accgaagtgt ttcggtcgtc gacgcctacg caatgaccgt cgaagccgac atcgagatgc
     6541 ctgtcgaacc gcatttggtg accgccacgc agcgacgcgc ctacgtgacg atctccgggc
     6601 accactatga ctcgatcgcg gtcatcgaca ccgagaacaa gcaggtcatc gccaaccatc
     6661 aggttccggg tgtcgcgacg gacgttgcgg tgagcccgga cgccacccgg atcttcgtcg
     6721 gccgggtggg cgacgacagc gttggcctcg ccgtgatcaa tacgtttggt cggacacaca
     6781 tactcgacat cgccgacggc ctcggtcgca cggtcgacgc tgtgcgggtc agtcccaacg
     6841 ggcgcatcgt gtatgtcgcc acctccgacg ccgacagcgg cacgctgttt cttgtcgacg
     6901 cggcggcggg ccggatgatt cgcgaggttc ccatcgcatc gccgatcagg gatatcgtgc
     6961 tgagccgcga cggcgcgctg gcctacgtcg ccagtttcga cacgcattgg ggcggctccg
     7021 tcgacatcat cgacaccgcc atcaacgaga tcaccgc
//
LOCUS       DASYLX010000135         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2748433,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000135 DASYLX010000000
VERSION     DASYLX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748433"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGP27_03265"
     CDS             <1..511
                     /locus_tag="VGP27_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV7850370.1"
                     /translation="DIAYASDHGPDGPAPETPDVILLGGAGPEYNHLTLSRVYDWMAQ
                     GVPVVAMHRSTSWNTTEGLRIDTGMYLIGMEETSGRKASAVGKPAPAGFLAAASRLGV
                     DPDEMYMIGDDLNNDVLAAQVVGMTGVLVRTGKFRQDTLDRWAADEFAMQPNHVIESV
                     ADLPEVLGL"
     gene            550..1044
                     /locus_tag="VGP27_03270"
     CDS             550..1044
                     /locus_tag="VGP27_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850371.1"
                     /translation="MTEEHVSVSTTINAPLDAVFAVLADPTTHQAIDGTGWVRESLDG
                     KPVTEVGQVFRMGMHHDNHPEGYYEMANRVEVFDPARAIAWQPGQGPDERGNLSGNSE
                     LIFGGWIWRYDLRPLGDDRTEVTLTYDWSGVPPNLRDIPFPPFDHQHLDNSLKHLAEL
                     AEAR"
     gene            complement(1041..>2757)
                     /locus_tag="VGP27_03275"
     CDS             complement(1041..>2757)
                     /locus_tag="VGP27_03275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7850372.1"
                     /translation="RVDSAPDAQALRSWQRRALVRYLAAQPRDFLAVATPGSGKTAFA
                     LRVAAELLADGVVEQITVVVPTEHLKTQWALAASRVGIALDPKFSNSAAHTSSEYHGV
                     VVTYAQVASHPTRHRVRTENRRTLVVFDEIHHGGDAKSWGDAIREAFDDATRRLALTG
                     TPFRSDDSAIPFVKYEPDAEGLMRSMADHTYGYAEALADSVVRPVVFLAYSGEARWRT
                     SAGEEHAARLGEPLTAEQTARAWRTVLDAKGEWIPAVLQAADTRLTQLRNGGVPDAGA
                     MVIASDQKAARAYAAVLTRLTGETPTVVLSDDPGSSARISEYSAGTGRWLVAVRMVSE
                     GVDVPRLAVGVYATSASTPLFFAQAIGRYVRSRQPGETASIFLPSVPTLLDLASEMEA
                     QRDHVLGKPHRESQGFDDEALEDAASKKSEPDADSGFESLGADAELDQVIFDGSSFGT
                     ATPAGSDEEADYLGIPGLLDAEAMRDLLRRRQEEQMTRRSRAVGDIGPGTASHPVRVS
                     THGQLRELRSQLNALVTVAHHRTGKPHGWIHKELRRICGGPPIAAATSDQLKARIEAV
                     RELRT"
BASE COUNT          433 a          956 c          938 g          430 t
ORIGIN      
        1 cgacatcgcc tatgcaagcg accacggtcc ggacggccct gcgccggaga ccccggatgt
       61 gatcctgctc ggcggtgcgg gcccggagta caaccacctg acgctcagtc gtgtctatga
      121 ctggatggcc cagggcgtgc ccgttgtcgc catgcaccgc agcacatctt ggaacaccac
      181 cgaggggctg cgcatcgaca ccggcatgta cctgatcggc atggaggaga cgtcgggacg
      241 caaggcgagc gcggtcggca agccggcccc cgccggattt ctcgccgccg cttcgcgtct
      301 cggcgtcgac cccgacgaga tgtacatgat cggcgacgac ctcaacaacg acgtgctggc
      361 cgcgcaggtg gtcggaatga ccggcgtcct ggtgcgcacc ggcaagttcc gtcaggacac
      421 cctcgaccgg tgggccgccg acgagttcgc gatgcagccc aaccacgtca tcgaatcggt
      481 cgccgacctg cctgaggtgt tgggcctgta aagcgatgag tatccggtga gtcgacggtc
      541 tgcactgaca tgactgaaga acacgtgagc gtgagcacca cgatcaacgc acccctcgac
      601 gccgtgttcg cggtgctggc cgacccgacg acgcaccagg cgatcgacgg caccggctgg
      661 gtgcgcgagt cgctggacgg caaacccgtc accgaagtgg gtcaggtttt ccggatggga
      721 atgcatcacg acaaccatcc ggagggctac tacgaaatgg ccaatcgagt cgaggtgttc
      781 gacccggcgc gcgcgatcgc gtggcagccg ggtcagggac ccgacgagcg cggcaacctg
      841 agcggcaact ccgagctcat attcgggggt tggatctggc gctacgacct gcggcccctc
      901 ggggacgacc gcaccgaggt cacgctgacc tacgactggt cgggagtgcc gccgaacctt
      961 cgcgatatcc cattcccgcc gttcgatcac cagcacctgg acaactcgct caagcatctg
     1021 gcagagctgg cggaggcgcg ttaggtgcgc agctcgcgca ccgcctcgat gcgggccttc
     1081 agctgatccg aggtcgccgc cgcgatcggc gggccgccgc agatccggcg cagctccttg
     1141 tgaatccagc cgtgcggctt gcccgtccgg tggtgtgcca ccgtgacaag cgcgttgagt
     1201 tggctacgca gttcgcgcag ctgcccgtgc gtcgacaccc ggacaggatg agacgcggta
     1261 cccggcccaa tgtcgccgac tgcgcggctc cgacgtgtca tctgctcttc ttgcctgcgc
     1321 cgcaacaggt ctcgcatcgc ttcggcatcc agcagacccg ggatgccgag ataatcggcc
     1381 tcctcgtcgc tgcccgccgg tgtcgcggtg ccgaacgacg agccgtcgaa gatgacctga
     1441 tcgagttcgg cgtccgcgcc cagcgactcg aacccgctgt ccgcgtcggg ctcggacttc
     1501 ttgcttgccg cgtcttcgag cgcctcgtcg tcgaagccct gcgactcgcg gtgcggcttg
     1561 ccgagcacat ggtcgcgctg cgcctccatc tcgctggcca ggtcgagcag cgtcggcacc
     1621 gacggcagga agatgctggc ggtctccccc ggctgccgtg aacggacgta gcggccgatc
     1681 gcctgcgcga agaacagcgg tgtggacgca ctggtggcgt agacgcccac cgccagccgc
     1741 ggcacgtcga cgccctcgga gaccatccgg accgcgacga gccaacggcc ggtccccgcc
     1801 gagtattcgg agatgcgtgc cgacgagccg gggtcgtcgg acagcacgac ggtcggcgtc
     1861 tcacccgtca gcctggtgag gacggctgcg taggcgcgcg cggccttctg gtccgacgcg
     1921 atgaccatgg cgccggcgtc gggcacgccg ccgttgcgca gctgcgtgag ccgggtgtcg
     1981 gcggcctgca gcaccgccgg tatccactcg cccttcgcgt cgagcaccgt gcgccacgcc
     2041 cgcgccgtct gctcggcggt cagcggctcg ccgagccggg ccgcatgttc ctcgcccgcg
     2101 cttgtgcgcc agcgcgcttc gccggaatag gcgaggaaca ccaccggccg gaccacgctg
     2161 tcggccagcg cctcggcgta gccgtaggtg tggtcggcca tcgagcgcat cagcccttcg
     2221 gcgtcgggtt cgtatttcac gaacgggatc gcggagtcgt cgctgcggaa cggtgtgccg
     2281 gtgagcgcga gccgccgcgt ggcatcgtcg aaggcctctc ggatggcgtc gccccaactc
     2341 ttcgcgtcac cgccgtggtg gatctcgtcg aacaccacga gcgtgcgacg gttctcggtg
     2401 cgcacccggt gccgggtcgg gtggctggcg acttgggcgt aggtgacgac gacgccgtgg
     2461 tactccgacg aggtgtgggc cgcggaattg ctgaacttcg gatcgagcgc aatccccacc
     2521 cgggaggcgg cgagcgccca ctgcgtcttg aggtgttcgg tgggcaccac gacggtgatc
     2581 tgctcgacga cgccgtcggc cagcaactcc gctgccaccc gcagggcaaa cgccgtctta
     2641 cccgaaccgg gagtcgcgac ggccaggaaa tcccgcggct gcgcggcaag ataccgcacc
     2701 aatgcccgac gctgccagct ccgcagagcc tgggcgtcgg gcgctgagtc aacccgc
//
LOCUS       DASYLX010000136         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1212925,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000136 DASYLX010000000
VERSION     DASYLX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212925"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGP27_03280"
     CDS             complement(<1..347)
                     /locus_tag="VGP27_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7850373.1"
                     /translation="MTRKPEISLMLRTFSDDANQDWNATVEMACAMDAAGVDRVVVSD
                     HLAFGEDLDAYGNPAVGGTAGGRQPTGPDGNWLEPLVFLTAVAAKTTRIRLATAVLLA
                     ALRRPAVLAKQLAT"
     gene            complement(344..1891)
                     /locus_tag="VGP27_03285"
     CDS             complement(344..1891)
                     /locus_tag="VGP27_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850374.1"
                     /translation="MPETSYAPCGDLSLAYQVFGRGPVELVWAGSFASHVELYWSMPE
                     FRSLMERLGTFARIILFDKAGVGLSDPVPKVRTLDERATEIEAVMDAAGFKKAAILGV
                     SEGGPAAILFAATRPERTRALILTGATAYAGWATWDDLERDPAELRARGIREVGENYA
                     PSIEQIAGFQRFGRAVKASWGSGNAAKCLLPSVKSMRQLGMFERMSASPGMARATLEA
                     AFQIDVRAILPTITVPVLVVHAREDPGVPIQSGRYLADHIRGARMLELDGTDHAPWFT
                     DPARIASEIEELLTGGHAAPPQSQRALRTVLFTDIVASTERAAATGDERWRAVLQRFG
                     EITAEASSSFGGTVVKSTGDGHLFTFDGPTQAIRCAEALRDGAETLGIEIRSGIHTGE
                     CELMDADIGGIAVHIAARILGEADGGDVLVSRTVRDLVVGSGMGFEDRGVVELRGVPG
                     SWELLAVNRHGAHGGSAEAKLASLPTPGPRAAMRRSDRAVAVMAKRAPWILRRAALLT
                     GNQGGQA"
     gene            1932..3422
                     /locus_tag="VGP27_03290"
     CDS             1932..3422
                     /locus_tag="VGP27_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7850375.1"
                     /translation="MTGSVAVIGAGPGGLVTARWLLSQGFEPSIFEQSHTLGGQWTGL
                     DGISGVWPTMHTNTSRILTSFSDLEHASDHVFPSNRDILAYLHRYAETFALASRIRFG
                     TRVENLSRDEGGWVIGHSGTTETFERVVVASGRFESPFIPSAPGLDTFTGSAGAMSTY
                     RYRNSTAYLGRRVLVAGGAISALEIASELAQLGADVTVTQRRQRYVLPKFAAGVPSDS
                     RIFTRYGTLANESLAAQEVDRQLKEIVVEAGGSPEQYGAPAPDASFFTAGVTLAQQYL
                     PLVAEGRITVRPWMISVDRRTVTFADGSAEAFDGILFGTGFQLDLPYLSDEISAILGL
                     DAVHLNADRFTFHPDLPGLAFVGMWDQSGGYFVPLELQARWVAYTWGGVVPEANEAEQ
                     RAAIDAYHARRRLPQKSRMNLVALAFARAAGVEPNPDEWPHLRRALLFGPLAPSCFRL
                     QGPDALPDASIRFVRDAAAFGAITSNELTDREEHYWSMVESARARC"
     gene            complement(3412..>4016)
                     /locus_tag="VGP27_03295"
     CDS             complement(3412..>4016)
                     /locus_tag="VGP27_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7850376.1"
                     /translation="LIEVAQIEVAACLAAEPVIEYSMNSVLQGREGNHSRGCLQGVYS
                     AAAEDDWVAVSVRDGTDLTRLGAILSRSDLKWGMAHDEFDAAVAEWARTRTAAEAVDS
                     LRAHQIPAEQVLTAERMYDLPQLAARNFYQEVEHPVTGPHRYPGWPFTVTPGPRRHHR
                     LPPPTLGQHNAEVLHGLGLTDDEIDELRASRVIGETALNT"
BASE COUNT          680 a         1354 c         1283 g          699 t
ORIGIN      
        1 gtcgccaact gcttggccag cactgccgga cgcctgagag ccgcgagcaa caccgcggtt
       61 gccagccgga tacgcgtggt cttggctgcc actgcggtca agaagacaag cggctcaagc
      121 caattgccgt caggacctgt cggctgcctg ccacccgcgg tgccaccgac cgcgggattc
      181 ccgtaagcat ccaggtcctc tccgaaggcc aggtgatccg agacgacgac gcgatccacc
      241 cccgcggcat ccatggcgca tgccatctca accgttgcgt tccagtcctg gttcgcgtcg
      301 tcgctgaaag tgcgcagcat cagcgagatc tcgggcttac gcgtcatgcc tggccgcctt
      361 ggttgccggt gagcagggcc gccctgcgca gaatccatgg ggcgcgcttg gccatcaccg
      421 cgacggcgcg gtcggaccgg cgcatcgcgg ctcgcgggcc aggggttggc aacgaggcca
      481 gtttcgcttc agccgaacct ccatgcgccc cgtggcgatt gactgcgagc agctcccagc
      541 tgcctggcac gccacgcaac tcgacgacgc cgcggtcctc gaaccccata cctgacccga
      601 cgaccaggtc gcgcacggta cgggagacga gaacgtcgcc accgtctgcc tcgccgagga
      661 tgcgcgccgc gatgtgcacc gcgattcctc cgatgtcggc atccatcaat tcacattcac
      721 cggtgtggat gccactgcgg atctcgatac ccaatgtctc ggcaccgtcg cgaagcgcct
      781 cggcacaccg aatggcctgc gtcgggccgt cgaacgtgaa gaggtgccca tcgcccgtgc
      841 tcttcaccac cgtgccgccg aagctcgacg acgcttccgc ggtgatctca ccaaaacgct
      901 gcagcactgc acgccagcgc tcgtcgcccg tagccgccgc gcgttcggtc gatgcgacga
      961 tgtcggtgaa caacacggtg cgcagggcgc gttgggattg cggcggcgcc gcgtggccac
     1021 cggtgagcag ctcctcgatc tcactcgcga tcctggcggg atcggtgaac cagggcgcat
     1081 ggtccgtgcc atcgagctca agcatgcgtg cgccgcgaat gtggtcggcg aggtagcggc
     1141 ccgactgaat cggaacgcca gggtcctcgc gggcgtgaac tacgaggacc ggcacggtga
     1201 tcgtcggcaa gatcgcgcgg acatcgatct gaaatgctgc ttcgagcgtt gcccgcgcca
     1261 ttcccgggct ggcggacata cgctcgaaca ttcctagctg gcgcatcgac ttgactgacg
     1321 gaagaagaca cttggcggcg ttgccgctgc cccacgacgc cttgaccgcg cgaccgaatc
     1381 gctggaaccc ggcgatctgc tcgatcgacg gtgcgtagtt ctcgcccact tcgcgtatgc
     1441 cgcgggcccg cagctcagcc gggtcgcgct cgaggtcgtc ccaggtggcc cagccggcgt
     1501 acgccgtcgc acccgtgagg atcaacgccc gcgttcgttc cggtcgggtt gcagcgaaga
     1561 ggatcgccgc tgggccgccc tcgctcacac cgaggatggc ggcctttttg aagccggcgg
     1621 catccatgac ggcctcgatt tcagtcgctc gttcgtcgag cgtgcgtact ttggggaccg
     1681 ggtcggacag gccgacccct gccttgtcga acagaatgat gcgagcaaag gtgccgagcc
     1741 tctccatcag agatctgaac tcgggcatcg accagtacag ctcgacatgg ctggcgaacg
     1801 atccggccca gaccagctcc accggcccgc gaccgaacac ctgatatgcc aggctgagat
     1861 cgccgcaggg tgcgtacgac gtttccggca cgtcgttgag cgtaattgac gcatgtgcga
     1921 cagttcggga gatgacagga agcgttgccg tgatcggggc ggggcccgga ggtctcgtca
     1981 cggcgcggtg gctgctgtcg caagggttcg agccgtcgat cttcgagcag agtcatacgc
     2041 tcggcggcca gtggacggga ttagacggca tcagcggcgt gtggccgaca atgcacacga
     2101 acaccagtcg gatcctgacg tcgttcagcg acctggagca cgcgagcgat catgtcttcc
     2161 cgtcgaaccg cgacatcctc gcctacctgc atcgctatgc cgagacattc gccctggcgt
     2221 ctcgcatccg gttcggcacc cgggtcgaga acctgagccg agacgaaggc ggctgggtca
     2281 tcggccattc cggcacgacc gagacgttcg agcgagtggt ggtggccagt gggcgattcg
     2341 aatcgccatt tatcccttcg gctccgggac tggacacgtt cacggggtcg gccggcgcga
     2401 tgtccactta tcgataccga aactcgacgg cctacctcgg aagacgtgtc ctcgtcgccg
     2461 gcggcgccat cagcgcgctc gaaatcgcct cggagctcgc ccaactcggc gcggacgtca
     2521 cggtcacgca gcgacgtcag cggtacgtgc tgccgaaatt cgctgcaggc gtcccgtccg
     2581 actcccgcat cttcacccgc tacggaactc tggcgaacga gtctctcgcc gcgcaagaag
     2641 ttgaccgaca gctcaaggag atcgtcgtcg aggccggtgg cagccctgag caatacgggg
     2701 ccccggcacc cgatgcgtct ttcttcaccg cgggcgtcac gctggcccag cagtatctgc
     2761 cgctggtggc ggagggccga atcacggtgc gaccctggat gatctcggtc gaccggagaa
     2821 ccgtgacctt cgccgacgga tcggctgaag ccttcgacgg aattctgttc ggcacgggat
     2881 tccaactcga tctgccatac ctgagcgacg agatcagcgc aatcctgggt ctcgacgccg
     2941 tgcatctgaa cgccgaccgc ttcaccttcc atcctgatct acccggcttg gcgttcgtcg
     3001 gcatgtggga ccagtccggc gggtatttcg tgccgctgga acttcaggcc cggtgggtcg
     3061 cctacacctg gggtggcgtg gttccggagg cgaatgaagc cgagcagcgg gcggcgatcg
     3121 acgcctacca cgcgcgtcgc cgactgcctc agaaaagccg catgaacctg gtggcgctgg
     3181 cgttcgccag ggctgcgggt gtcgagccga atcccgacga atggccgcat ctgcgccgcg
     3241 cgctgttatt cggcccgttg gcaccgagtt gctttcgcct gcagggtcca gacgccttgc
     3301 ccgacgcatc gattcgcttc gtacgggatg cggccgcctt cggcgcgatc acatccaatg
     3361 agctgaccga tcgggaagag cactattggt cgatggtgga gtccgcacga gctaggtgtt
     3421 gagcgcggtt tcgccgatga cccggcttgc gcgcagttcg tcgatttcgt cgtccgtcaa
     3481 gccgagtccg tgcaggacct ccgcgttgtg ctgccccagc gtcggcggcg gcaaccggtg
     3541 atgccggcgg ggtcccggcg tcaccgtgaa cggccagccc ggataccggt gcggcccggt
     3601 gaccgggtgc tcaacctctt ggtagaaatt ccgcgcagcc agctggggca ggtcgtacat
     3661 tcgctcggcg gtcagcacct gctctgccgg aatctgatgt gccctcaacg aatcaaccgc
     3721 ttctgcggcg gttcgcgtcc gcgcccactc ggctactgcg gcgtcgaact catcgtgggc
     3781 catcccccac ttcagatcgg agcgcgacaa gatcgcaccg agccgggtca aatcggtgcc
     3841 gtcgcgaacc gagacagcaa cccaatcatc ctcggcggcg gccgaataca cgccctgcag
     3901 gcacccgcgg ctgtgattgc cttcccgtcc ctgcaggacc gaattcatcg aatactcgat
     3961 caccggttcg gcggcgagac acgccgcgac ctcgatctgc gccacctcga tcagtt
//
LOCUS       DASYLX010000137         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_664975,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000137 DASYLX010000000
VERSION     DASYLX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664975"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VGP27_03300"
     CDS             complement(<1..680)
                     /locus_tag="VGP27_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850377.1"
                     /translation="MIGTGRGGRSRSISGGRLLWCRHRSHDGPARGKIEETEHIATTA
                     VALIIPSRQVVYPRGADCGRGHPASHTRATQPARFLQRIPDHIDHWAHTVDLSPCITG
                     VTLPYGVQDRSHQARPHEVVTVEVVGGGRDPGCSGCCDGLRPRSQREPRVPHPAVFDD
                     ASGGGEHLEHLIELSKSEGHDVVRLGLAPHPAGDPLPWRAAGHPPLLDPLTVVPELGA
                     IEDATMRRR"
     gene            1079..>2020
                     /locus_tag="VGP27_03305"
     CDS             1079..>2020
                     /locus_tag="VGP27_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HEV7850378.1"
                     /translation="MVTAVGQLRTGLGAPLAQATDAPSLQLHLGVVDTLLLFIYFVFV
                     IGIGFALKRAVATSTDFFLSGRSLPAWVTGLAFVSANLGAIEILGQTANGAQYGAATV
                     HYYWIGAIPAMAFLGIVMMPFYYGSKVRSVPEYLRLRYDQKAHLINATTFVLSSVLLA
                     GVNLYALASVIDALLGIPLPIAVVVSAGFVLSYILLGGLRSAIYNEVLQFFVILAGLI
                     PLVVIAVKEVGGLSELFSRLSTVKGTAFTSAWTGTGFGGNNPLGDWFGIIAGLGFCVS
                     FGYWTTNFAEVQRAMSAKDENAARMTPIIAAYPKALIP"
BASE COUNT          298 a          706 c          648 g          368 t
ORIGIN      
        1 cgcctccgca tcgttgcatc ctcgatggct cccaattcgg gaacgacggt cagcgggtcc
       61 aacaggggag ggtgtcccgc cgcacgccat ggcagcgggt cgcccgctgg atgcggggcc
      121 agacccagcc gtacgacgtc gtggccttcc gacttcgaca gctcgatgag gtgctcgagg
      181 tgctcgccgc cgcccgaagc gtcatcgaac acggctgggt gcgggacgcg tggttcgcgt
      241 tgcgaccgcg gtcgcagccc gtcgcagcac ccgctgcagc cagggtctcg tcctccccct
      301 actacctcga ccgtgacgac ctcgtggggg cgtgcctggt gggagcggtc ctgcacgccg
      361 tacggcagcg tcaccccggt gatgcaaggg ctgaggtcga cggtgtgggc ccagtggtcg
      421 atgtgatctg ggatgcgttg caggaatcgc gcgggctggg tggccctggt gtggctggcc
      481 gggtggccgc gcccgcagtc cgcgccgcgc ggatacacga cctgacgcga tggaatgatc
      541 aacgcgaccg cagtcgtagc gatgtgctcg gtctcctcga tcttgccgcg agccggacca
      601 tcatggctgc gatgacgaca ccagagcagt cgtccgccgg agattgaccg cgaccgcccg
      661 ccgcggccgg tgccgatcac cggaggacgc agctgctgca ggcgggtcct ctccggtgat
      721 tttctcgtcc tgtagcagct cgatcggctc gagaaccact caacccgccg gcaccgaccg
      781 cgacggctcg gctcggccag ttcggcgttc agcgcaccgc gacggtccgc ggagacgccg
      841 gtgcagtgag gatgcggcaa gaccgcgcga accccggtgg atgtcgacac tccgtcagcg
      901 tcttgcgcgg ccgcctgcgt ctccgggacc gacatgccgt ccgcgtcgga tgatcaccag
      961 ctacggcccc catccgctat cgcgcaccta cttgcaagac cactgatccg agtggtcgct
     1021 gtcatcgaca cctgccatgc tttgccgagc gcagctaccg cgggcgtgga gggccgatat
     1081 ggtcaccgca gttgggcagc ttcggacggg actcggcgcc cctctggcgc aagcaaccga
     1141 cgccccgagc ctgcaactcc atctgggcgt agtcgacacg ctcttgctgt tcatctactt
     1201 cgtcttcgtc atcggcatcg gcttcgctct caagcgcgcc gtcgcgacct cgactgactt
     1261 cttcctgtcc ggccgctcac tgccggcgtg ggtcaccggg ctggccttcg tctcggccaa
     1321 cctgggcgcc atcgagatcc tcggtcagac cgccaacggc gcgcagtacg gggcggcaac
     1381 ggttcactac tactggatcg gcgcgatccc ggcgatggca ttcctgggca tcgtgatgat
     1441 gccgttctac tacggctcca aggttcgatc ggtcccggag tacctgcggc tgcgctatga
     1501 ccagaaggcg catctgatca acgccaccac attcgtgctc tccagcgtgc tcctggccgg
     1561 ggtcaacctg tatgcgctcg ccagcgtcat cgacgctctg ctcggcatcc cgttgccgat
     1621 cgcggtcgtc gtgtccgccg ggttcgtgct ctcctacatc ctgctcggtg ggctgcgcag
     1681 cgccatctac aacgaggtcc tgcagttctt cgtcatcctc gccggcctca tcccactggt
     1741 ggtcatcgcc gtcaaggagg tcggcggcct ttcagagctg ttctccaggc tcagcacagt
     1801 caagggcacc gcgttcacgt cggcctggac gggcaccggt ttcggcggca acaaccccct
     1861 cggcgactgg ttcggcatca tcgcgggcct cggcttctgt gtctcattcg gctactggac
     1921 gaccaacttc gccgaggtgc agcgagcgat gtcggccaag gacgagaacg ccgcccggat
     1981 gactccgatc atcgcggcct accccaaggc gctgatcccg
//
LOCUS       DASYLX010000138         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2310904,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000138 DASYLX010000000
VERSION     DASYLX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310904"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..636)
                     /locus_tag="VGP27_03310"
     CDS             complement(37..636)
                     /locus_tag="VGP27_03310"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7850379.1"
                     /translation="MSSSHPMRRIEILLFDGLDELDVVAPFEILVSAGYRVDLVTVEP
                     CRRVDAAHGLTLTPTGVLGQPDLLVVPGGGWALKATKSAWSEVRRGVIPAAIAERHAA
                     GARIAGVCTGAMLIAAAGILRGRPAVTHRSALDDLRGFDVDVHPEARVVDAGDVLTSG
                     GVTSGIDLALHLIELDRGREAALKEAARIEHDYRGPVLT"
     gene            complement(664..1392)
                     /locus_tag="VGP27_03315"
     CDS             complement(664..1392)
                     /locus_tag="VGP27_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850380.1"
                     /translation="MSPVVLITGASRGVGAQIATQLADSALHVVVNYREKAKRADAVV
                     DAVRAAGGNASAVRADVTEEQAVATMLEGIDDRFGNLDVLVLNASGGLEPGADPGYPM
                     RLNRDAQVRLAQLALPLMRAGGRIVFVTSHLAHFYTRKPVPDEYLPIATSKQAGEKAL
                     RDMLPTFEAAGLTLVVVSGDMIEGSTMVRLFERRDLEAVQARRRAGDGLPTAAEFADA
                     IVRATHEPHRNGDTVYVGGPDYLS"
BASE COUNT          265 a          559 c          495 g          241 t
ORIGIN      
        1 ccggtacgca tagcagtacc cgttcacgag ctcggctcag gtcagaaccg gcccacggta
       61 atcgtgttca atccgggctg cctctttcag cgcagcttcc cggccgcgat cgagctcgat
      121 gaggtgtagc gccaaatcga tgcccgacgt gacacctccg ctggtgagca catcgccggc
      181 gtcgacgaca cgcgcttcgg ggtgtacgtc cacatcgaat ccgcggaggt cgtcgagggc
      241 cgatcggtgg gtgaccgccg gtctgccgcg cagtattccg gcggcagcga tcaacatcgc
      301 cccggtgcag acacccgcga tgcgcgcacc cgccgcgtgc cgctcggcga tcgccgcggg
      361 aatgacgccg cgacgcacct cgctccatgc gctcttggtg gccttcaacg cccagcctcc
      421 gcccgggaca acgagcaggt ccggttggcc caggacgccg gtgggggtca gtgtcaatcc
      481 gtgcgcagcg tcgacgcgac ggcagggttc cacggtgacg aggtcgactc gataacccgc
      541 cgacaccagg atctcgaacg gcgcgacgac gtcgagttcg tcgagcccgt cgaacagcag
      601 aatctcgatg cgccgcatcg gatggctcga agacacgact cctcctatca ccgatctcgg
      661 gggtcaggac aagtagtccg ggccgcccac gtacaccgtg tcgccgttgc gatgcggctc
      721 atgcgtggcg cgaacgatcg catcggcgaa ctccgcggcg gtcggcaacc cgtcgcccgc
      781 acggcgccgc gcctgcaccg cctcaagatc acgccgctcg aacagacgca ccatggtgct
      841 gccttcgatc atgtcgccgg acaccacgac caacgtcagc ccggccgcct cgaacgtcgg
      901 aagcatgtcg cgcaacgctt tctcgccggc ctgcttgctt gtggcgatcg ggaggtattc
      961 gtcgggaacc ggtttgcggg tgtaaaagtg cgccagatgg ctggtgacga acacgatccg
     1021 accgccggcg cgcatcagcg gaagtgccag ctgagccagt cgcacctggg catcgcggtt
     1081 gagccgcatc ggatagccgg gatcagcccc gggctccaac ccgccggatg cgttgagcac
     1141 caacacgtcc aggttgccga accgatcgtc gatgccttcg agcatcgttg ccaccgcctg
     1201 ctcctcggtg acatcggcgc ggacagcact ggcgttgccg cccgcggccc gaaccgcatc
     1261 gacgacggca tccgcacgct tggccttctc ccggtagttg acgacgacgt ggagggccga
     1321 atccgcaagc tgcgtcgcga tctgcgcccc tacgccacga gaggcgccgg tgatcaggac
     1381 gactgggctc acgacccgtg acgcgccatg gcctcggcca ggtcgtagtc gacggccacg
     1441 ccgccccacg cctcaccggc gccgtacgcc accagcgtca tcctgcccgt ctgcggagcg
     1501 gacttctcgg ccacacagct gcccagagcc agcggtatcg atgccgccga cgtattgccg
//
LOCUS       DASYLX010000139         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2420096,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000139 DASYLX010000000
VERSION     DASYLX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420096"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VGP27_03320"
     CDS             complement(<1..173)
                     /locus_tag="VGP27_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7850381.1"
                     /translation="MAWDFSTDPEWAEQLTWVEQFVRSECEPIDLIVKESHDLNDPVR
                     QALIPPLQQIVKDR"
     gene            complement(195..1334)
                     /locus_tag="VGP27_03325"
     CDS             complement(195..1334)
                     /locus_tag="VGP27_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV7850382.1"
                     /translation="MSGPLHGVRIVTMGGLGPGPFCGMLLGDLGADVVRVDIPRAVDG
                     LLPIDYTVRRSQRSIALDVKDAAGRAILLKLVENADAFVEVFRPGVAERLGIGPDDLH
                     ERNPRLVYARMTGYGQDGPYAERAGHDIKYIALAGALHGIGTASAPVPPLNLVGDYGG
                     GGMLLAVGLLSAILEARASQEGQVVDVAMVDGAATLMTPYYGMVAAGTWRDQRYDNVL
                     DGAAHYYGVYPTADERHVAVGAMEPQFYAELCQLLGVDIPQDANDPTAWDAHRAVLAA
                     RFREKSRTEWEELLSSPGCCATPVLSLAEAPRHPHNVARGTFVEIDGVVQPAPAPRFS
                     RTSPPTPTGPSLPGDHTRDLLGGLGMDDKAIAELLASGVARQSSD"
     gene            complement(1366..2133)
                     /locus_tag="VGP27_03330"
     CDS             complement(1366..2133)
                     /locus_tag="VGP27_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7850383.1"
                     /translation="MTETLLVERPHAGVLVVTLNRPKRLNAINAVMRRELIETLESVG
                     HDVNAVVLTGAGRGFCSGIDMRDFGPDVPTADDPAIDRLRFQESMAALPQAIRDLPQP
                     VIAAVNGPCVGGGLALCLAADIRICSSAATFGNAAILLGLSGAEMGMSYHLPRIVGTS
                     VAADWMLTGRTVSADEADGRGLVSELVEPDRLADRAVEIAGAIAELSPLGVQLTKRAL
                     QVNTDASGLDAAVELENRNQVLSHATDDAAARRQKWS"
BASE COUNT          342 a          785 c          735 g          368 t
ORIGIN      
        1 cggtccttga cgatctgctg cagcggcggg atgagcgctt ggcgcaccgg gtcgttgagg
       61 tcgtgtgact ccttgacgat caggtcgatc ggctcgcact ccgagcgcac gaactgctcg
      121 acccaggtca gctgctccgc ccattccggg tcggtcgaga agtcccacgc catcgctcgt
      181 ccttccttcg aagttcagtc gctgctctgt cgcgctacac ctgatgcgag aagctccgcg
      241 atcgccttgt cgtccatccc cagcccgccc agcaggtcgc gggtatggtc gccgggcagc
      301 gacgggccgg tcggtgtcgg aggcgaagtc cgcgagaacc gcggcgccgg tgccggttgc
      361 accacgccgt cgatttcgac gaaggtgcca cgggccacgt tgtgcggatg ccgcggtgct
      421 tcggcgaggc tgagcacggg tgtggcacag catcccggag aactgagcag ttcttcccac
      481 tccgtgcgcg acttctcgcg aaaccgcgcc gccagcacag cccggtgagc gtcccacgcc
      541 gtcggatcgt tcgcgtcctg cgggatgtcg acgccgagga gctgacacaa ctcggcgtag
      601 aactggggct ccatggcgcc gactgcgaca tgcctctcgt cagcggttgg gtagacaccg
      661 tagtagtgcg ccgcaccgtc aagtacgttg tcgtaccgct gatcccgcca tgtgcccgcc
      721 gccaccatgc cgtagtacgg cgtcatcagc gtggcagcgc cgtcgaccat cgcgacgtcg
      781 acgacctggc cctcctgcga agcccgtgcc tccaggatgg cgctgagcaa cccgacggcc
      841 agcagcatgc caccgccgcc gtaatcaccg accaggttca gcggcggcac cggcgcggag
      901 gcggtgccga tcccgtgcag cgcgcccgcg agcgcgatgt acttgatgtc gtggccggcg
      961 cgctcagcgt acggcccgtc ctgtccgtaa ccggtcatcc gggcgtacac caggcgcggg
     1021 ttgcgctcgt gcagatcgtc gggcccgatg ccgagccgct cagccacgcc gggtcggaac
     1081 acctcgacga atgcgtcggc gttctcgacc agcttcaaca ggatggcccg gcccgcagcg
     1141 tctttgacgt ccagcgcgat ggaacgctgg ctgcgccgga cggtgtagtc gatgggcagc
     1201 agaccgtcga ccgcacgagg aatgtcgaca cgcacgacgt cggcgccgag gtcaccgagc
     1261 aacatgccgc agaacgggcc aggcccgaga ccgcccatcg tcacgatccg gacgccgtgc
     1321 agcggtccgc tcactgccgt ctgctcttcg cgccagcgct catcactacg accacttctg
     1381 ccgccgcgcg gccgcgtcgt cggtggcgtg actcagcact tgattgcggt tctccagttc
     1441 caccgccgca tcgagtcccg aggcgtcggt gttgacctgt agtgcacgct tggtcagttg
     1501 cactcccagc ggcgacaatt cggcgatggc gcccgcgatc tcgacggcgc ggtcggccag
     1561 ccggtccggt tctaccagct cgctcaccag cccgcggccg tcggcttcgt ctgctgacac
     1621 ggtgcgaccg gtcagcatcc agtcggcggc cacgctggtt ccgacgatcc tcggaaggtg
     1681 gtaactcatt cccatctcgg cgccggacag cccgagaaga atcgcggcat ttccgaatgt
     1741 tgccgcgctg gagcagatcc ggatgtcggc ggccaggcac agcgcgagcc cgccgccgac
     1801 gcacggaccg ttgaccgcgg cgatgacggg ttgcggcaga tcccggatcg cctgcggcag
     1861 cgctgccatc gactcttgga atcgcagccg gtcgatcgcg ggatcgtctg ccgtgggcac
     1921 atcgggcccg aaatcgcgca tgtcgatgcc cgaacagaag ccgcgaccgg cgccggtcag
     1981 gacgacggcg ttgacgtcgt ggcccaccga ctcaagcgtc tcgatgagtt cgcgccgcat
     2041 cactgcgttg atcgcgttca gccgcttggg ccggttcagc gtcacgacga gcacgcccgc
     2101 gtgcggccgt tcgacgagca gcgtctcggt cacccgactc agcgccgcgg caagccgagc
     2161 acctgcgtgg cgatgatgtt gcgctggatc tcggacgtgc cgcccgcgat cgtcccgccg
     2221 aagctgcgtg
//
LOCUS       DASYLX010000140         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2529506,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000140 DASYLX010000000
VERSION     DASYLX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529506"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1110
                     /locus_tag="VGP27_03335"
     CDS             100..1110
                     /locus_tag="VGP27_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850384.1"
                     /translation="MRVILDQDQRDLAAMCRSLLAAELEPDAWKTLADAGVFALPLDG
                     NLTDVGIFCVEAGRALCSRAVQSTVYSALAMDWLGVRAWLPGLTAGEARATTALFDPR
                     DASDVTPRLQARADGDRWRVSGVADFVADADVAAVVVVSTDAGVTLVDTSTPGIRIEP
                     VPLMGGQAFTVRFDEVVVDDVAAAGADRLRRFANAVVALDCLDLVGVGEAVIDRTVTY
                     TKMREQFGRPIASFQAAQHLVADMHIALTAARLAAQSAVFWIGQGRTGTRETAVARMR
                     AAEVKKITLDAHQLHGGMGYVVDTGLYRYSERARVLSTVGGGADIAAKWLEDEMNWEG
                     RR"
     gene            1107..1619
                     /locus_tag="VGP27_03340"
     CDS             1107..1619
                     /locus_tag="VGP27_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850385.1"
                     /translation="MTGGSRGSLIDPESAERIGRVVAVVTGEVYRRDWQRWAAAVGDH
                     NPLWFDPDYARRHGYRDIVCPPLYLQYSILGVAALGDLRPDGSSGAVTGSMAFPRAPR
                     RMAGGESTTFHLPAYHGDAVEMTRTVDSIVEKEGRSGRFVLVTWRTEYRNQHRDLLAE
                     ATTSMIARPA"
     gene            1616..2095
                     /locus_tag="VGP27_03345"
     CDS             1616..2095
                     /locus_tag="VGP27_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEV7850386.1"
                     /translation="MTPPTPAREGTQVSFEDVDAGDVIPALTVTVDETQMFFFSAATY
                     NGHRIHYDKEWARDREGYDNVLVQGPLQAALLSRALTDWIGGDGRLVAFSVQNRAIAY
                     PGQELTFGGVVTGKPCDARASTEDGCGLVDLDIFCRCGGDVLMPGTATVTLPHRSAG"
     gene            2092..3297
                     /locus_tag="VGP27_03350"
     CDS             2092..3297
                     /locus_tag="VGP27_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7850387.1"
                     /translation="MTGLRGEAAIVGIAELPAEKKPSGPPSFTLDQYARLAKMVIEDA
                     GVDPAVVNGLSTHGIGESTMFAPATLSEYLGMALDFGDRVDLGGATAAGMVWRAAAAV
                     ELGQADAVLVVMPGSTEVPRSEAKPGRTLSWYGASSNNYGSPQAEFEIPYGNVGQNGP
                     YAQIAQRYAHEFGYDATALARIAVHQRVNACAHPGAVFHGKPITVDDVLNSPVIADPI
                     HMLETVMRVQGGAGVLIASADVARRSRHRPVWIKGFGEHIRFKTPTYADDLIRTPIAR
                     AAERAFSMAGVQRSDIDMASIYDCYTITVLMTLEDAGFCGKGDGMKWLTERDLTFRGD
                     FPINTAGGQLSYGQAGMAGGMHHVVDGARQVMGRSDQAQVRDCNTAFVTGNGGIMSEQ
                     VALILGGDR"
BASE COUNT          525 a         1056 c         1230 g          532 t
ORIGIN      
        1 tgtcgccgct gatgcggttc ggcggcggca ccaacgaagt cctgcgcgac gtcatcgccc
       61 agcgcggtca cacaatgccc tcgtacggtc ggtgacgcga tgcgagtgat cttggatcag
      121 gaccaacgcg atctggccgc gatgtgccgc agcctgctgg cggcagaact cgagcccgac
      181 gcgtggaaga cgctggccga cgcgggcgtg ttcgcactgc ctctcgacgg caacctcacc
      241 gacgtcggga tcttttgcgt cgaggccgga agagcgttgt gttcgagagc cgttcagagc
      301 acggtgtact cggcactggc catggactgg ctcggtgtgc gggcttggct gcctgggctc
      361 acggccggcg aggcgcgcgc gacgacggcg ctgttcgacc cgcgggatgc ctccgacgtg
      421 acgccgcggc tgcaggcgcg ggctgacggc gaccgctggc gggtgagtgg tgtagcggat
      481 ttcgtcgccg atgcggatgt cgcggccgtg gtggttgtca gcaccgacgc aggtgtgacc
      541 ctcgttgaca cctcgacgcc cggcatcagg attgagcccg tgccgctgat gggcggccag
      601 gcattcacgg tgcggttcga cgaagtggtg gtcgacgacg tagcggcggc cggtgcggac
      661 cgtttgcgcc gcttcgccaa tgccgttgtg gcgctggact gcctggacct tgtcggcgtc
      721 ggcgaggcgg tgatcgaccg cacggtgacc tacacgaaga tgcgtgaaca gttcggcagg
      781 ccgatcgcgt cgtttcaggc ggcacagcat ctggttgccg acatgcacat cgcgctgacg
      841 gcggcccggc tcgccgcgca gtcggcggtg ttctggatcg ggcagggccg caccggcacg
      901 cgcgaaacgg cggtagcgcg gatgcgcgcg gccgaggtca agaagatcac gctggacgca
      961 catcagttgc acggcggcat gggctatgtg gtcgataccg gcttgtaccg gtactccgaa
     1021 cgcgcgcggg tgctgtcgac ggtgggaggc ggtgccgata tcgccgcaaa atggcttgag
     1081 gacgagatga actgggaggg ccggcggtga ccggaggttc tcggggcagc ctcatcgatc
     1141 cggagtcggc ggagagaatc ggccgggtcg tcgccgtcgt gaccggcgag gtgtaccggc
     1201 gggactggca gcggtgggcg gcggcggtcg gcgaccacaa cccgctgtgg ttcgaccccg
     1261 actacgcgcg ccggcacggc taccgcgaca tcgtctgccc gccgttgtat ctgcagtact
     1321 cgattctggg tgttgcggcg ctcggcgacc tgcggccgga cggctcgtcg ggcgcggtga
     1381 cgggcagcat ggcgtttcct cgtgcgccta ggcggatggc cggtggcgag agcacgacgt
     1441 ttcatctgcc cgcctaccac ggcgacgcgg tcgagatgac gcgcaccgtc gactcgattg
     1501 tcgaaaagga gggcaggtca ggcagattcg tgttggtgac gtggcgcacc gagtaccgca
     1561 accagcaccg cgacctactc gccgaggcca ccacgtcgat gatcgccagg cccgcatgac
     1621 tccccccact cctgctcgcg agggaacgca ggtgtccttc gaggacgtcg acgccggtga
     1681 cgtgatcccg gcgctgacgg tcaccgtcga cgagacgcag atgttcttct tcagcgccgc
     1741 tacctacaac gggcatcgca tccactacga caaggagtgg gcgcgcgacc gcgaaggcta
     1801 cgacaatgtg ctcgtgcagg gcccgctgca ggcggcgctg ttgtcgaggg cgctgacgga
     1861 ctggatcggc ggcgacgggc gactggtggc gttctcggtg cagaaccgcg cgatcgccta
     1921 tcccggccag gagttgacgt tcgggggtgt cgtcaccggc aagccctgcg acgcgcgcgc
     1981 gagcaccgaa gacggctgcg gcctggtcga tctcgacatc ttctgcaggt gcggcggcga
     2041 cgtgctgatg ccgggcaccg ccaccgtcac gttgccgcat cggagcgcgg gatgacgggt
     2101 ctgcgcggcg aagcagccat cgtcggcatc gcggagttgc ccgcagagaa gaagccgagc
     2161 ggcccgccgt cgttcacgct cgaccagtac gcgcggctgg cgaagatggt catcgaggac
     2221 gccggcgtcg atcccgctgt ggtgaacggg ctttcgacgc acggcatcgg cgagtcgacc
     2281 atgttcgcgc cggccacact gtcggagtac ctgggtatgg cgctggactt cggcgaccgt
     2341 gtcgacctgg gcggggcaac cgcggcgggc atggtgtggc gggcggccgc ggccgtcgag
     2401 ctgggacaag cagatgcggt gctcgtcgtc atgcccgggt cgacggaggt tccgcgatcg
     2461 gaggcgaagc ccggccggac gctcagctgg tacggcgcgt cgagcaacaa ctacggctcc
     2521 ccgcaggccg agttcgagat tccgtacggc aacgtcgggc agaacggccc gtacgcgcag
     2581 atcgcgcagc gctacgccca cgaattcggc tatgacgcaa cggcattggc gcggatcgcg
     2641 gtgcaccagc gggtgaacgc gtgcgcccac cccggtgcgg tgttccacgg caagccgatc
     2701 accgtcgacg acgtgctgaa cagcccggtg atcgccgacc cgatccacat gctcgagacg
     2761 gtgatgcggg tgcagggcgg cgcgggtgtg ctgatcgcga gcgccgacgt cgcccggcgg
     2821 tcccgtcatc gcccggtgtg gatcaagggt ttcggcgagc atatccggtt caagacaccg
     2881 acttacgccg acgatctcat ccgcaccccg atcgcccgcg cggccgaacg ggcgttctcg
     2941 atggcaggcg tgcagcggtc cgacatcgac atggcgtcga tctacgactg ctacacgatc
     3001 accgtgctga tgacgctgga agacgcgggt ttctgcggca agggcgatgg catgaagtgg
     3061 ttgacggagc gcgacctgac cttccgcggc gacttcccga tcaacaccgc gggcggacag
     3121 ctgtcgtatg gccaggccgg catggcaggc ggcatgcacc atgtggtcga cggcgcgcgc
     3181 caggtgatgg gccgctcgga ccaggcgcag gttcgcgact gcaacacggc gttcgtcacc
     3241 ggcaacggcg gcatcatgag cgagcaggtc gcgctgatcc tgggtggcga ccgatgagcg
     3301 cgccgttgcc ggagccgacg ccggtctcgc agccgttctg gga
//
LOCUS       DASYLX010000141         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_116806,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000141 DASYLX010000000
VERSION     DASYLX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116806"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VGP27_03355"
     CDS             <1..155
                     /locus_tag="VGP27_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV7850388.1"
                     /translation="VFRMAPFHHHFELVGWAETTVIIRFWLLTAIACGLGVALYYSEW
                     LTTVGA"
     gene            157..1581
                     /gene="murD"
                     /locus_tag="VGP27_03360"
     CDS             157..1581
                     /gene="murD"
                     /locus_tag="VGP27_03360"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV7850389.1"
                     /translation="MTRQAVAPLVPGARVLVTGAGITGRSVSAALEPTGVRLTICDDD
                     PLALQRLVTPATVVTTDEAVAHVADYDLVVTSPGFPPTAPVLAAAAAAGLPIWGDVEL
                     AWRLDRAGWFGTPKRWLVVTGTNGKTTTTSMLHAMLVAAGRRAVLCGNIGNPVIDMLG
                     EPAELLAVELSSFQLHWAPSLRPEAGAVLNVAEDHLDWHGSMDAYAADKARALNGRVA
                     VVGLDDPVAAGLLPQAAASVRVGFRLGNPAEGELGVRDGTLIDRAFADDLPLADTASI
                     PVPGPVGVLDALAAAALARAVGVPAPAIADALSSFHVGRHRAEVVAEVDGVTYVDDSK
                     ATNPHAAHASIAAYPRVVWIAGGLLKGASVDDVVSEVSTRLVGAVLIGRDRALVANAL
                     SRHAPDVPVVEVVTGEDSVVSETNESDVNSVTRLMTAVVAAARRLARPGDTVLLAPAG
                     ASFDQFSGYAERGDAFAAAVRATG"
BASE COUNT          212 a          565 c          574 g          266 t
ORIGIN      
        1 gggtgttccg gatggcgccc tttcaccatc acttcgaact cgtcggctgg gcggaaacca
       61 cggtgatcat ccgcttctgg ctattgaccg ccatcgcgtg cggcctcggt gtggcgcttt
      121 actacagcga gtggctcacc acagtcgggg catgacgtga cccgacaagc cgtcgcgcca
      181 ctcgtgcccg gcgcgcgcgt gctggtgacc ggggcgggca tcaccggccg ctcggtgagc
      241 gcggcgctgg aaccgacggg tgtccggctg accatctgcg acgacgaccc actcgccctg
      301 cagcgcctcg tcacgcctgc gaccgtcgtc accacagacg aggccgtcgc gcacgtcgcc
      361 gactacgacc tcgtcgtcac cagccccggg ttcccgccca ccgcaccggt gctggctgcc
      421 gccgccgccg cgggcctgcc gatctggggc gacgtcgagc tggcatggcg actggaccgg
      481 gcgggctggt tcggaacgcc gaagcgctgg ctggtggtga cgggcaccaa cggcaagacg
      541 accaccacgt cgatgctgca cgcgatgctt gtcgccgcgg gcaggcgcgc ggtgttgtgc
      601 ggcaacatcg gcaacccggt gatcgacatg ctcggcgagc ccgccgagct gctcgcggtc
      661 gagctgtcga gcttccagct gcactgggcg ccgtcgctgc ggccggaggc aggcgcggtg
      721 ctcaacgtcg ccgaggacca tctcgactgg cacggttcca tggatgcgta cgcggccgac
      781 aaggcgcggg cgctgaacgg ccgggtcgcc gtcgtcgggc tcgacgatcc ggttgcggcc
      841 ggtctgctgc cgcaggccgc ggcatcggta cgggtcggct ttcggctcgg caatccggcc
      901 gaaggggagc tgggggtgcg cgatggcacg ttgatcgaca gggccttcgc cgacgacctg
      961 ccccttgccg acacggcttc cattccagta ccggggccgg tcggtgtcct cgacgcgttg
     1021 gccgccgcgg cgctggcccg tgccgtcggc gtgcccgcac cggccatcgc cgacgcgctc
     1081 tcgtcgtttc acgtcggtcg ccaccgcgcc gaggtggtcg ccgaggtgga cggcgtcacc
     1141 tacgtcgacg actccaaggc caccaacccg cacgccgcgc acgcgtcgat cgccgcctac
     1201 ccgcgggtgg tgtggatcgc cggcggtcta ctcaagggcg cttcggtcga cgacgttgtc
     1261 tccgaggtgt caacgcgcct ggtcggagcg gtgctgatcg gccgggaccg ggcgctggtc
     1321 gccaacgcgt tatcgcgaca cgccccggat gtacccgttg tggaggttgt gacgggggag
     1381 gattctgtgg tgtctgagac caatgagtca gatgtgaatt ctgtgactcg cttgatgact
     1441 gccgtggtcg ccgccgcacg caggttggcc cgccctggcg acaccgtgct gctggctccc
     1501 gcgggcgcat ccttcgacca gttcagcggt tacgccgaac gcggagacgc cttcgcggcc
     1561 gccgtccgcg cgacggggta gcagtgagca ccattctgac ccggttgcgg ctccgcc
//
LOCUS       DASYLX010000142         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2529606,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000142 DASYLX010000000
VERSION     DASYLX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529606"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGP27_03365"
     CDS             complement(<1..688)
                     /locus_tag="VGP27_03365"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEV7850390.1"
                     /translation="MSKTACLLGAVAVLVLAGCGSDEEKESIGDPLSRAPKGDLSTTG
                     GAARLGVDQTGTIRDSISGSASRNVILLIGDGMGDSEITLARDYAEGAGGFFDGIDAL
                     PLTGSYTTYALTKDGKPDYVADSASSASGWTTATKTYDGALAVNIKGDPQKTILELAK
                     EHGFATGDITTSEVQDATPAALISHISERKCYGPEETAKKCATETIENGGLGSVTEQL
                     LAVRPDLTMGG"
     gene            complement(798..1952)
                     /locus_tag="VGP27_03370"
     CDS             complement(798..1952)
                     /locus_tag="VGP27_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7850391.1"
                     /translation="MLNTVAIRGYRSLRDIVVPLTGLTVVTGANGTGKSSVYRALRLL
                     ADCGRGEVIGSLAREGGLESVLWAGPETTGGARRTGATEGTTRTRPVALELGFAADDF
                     GYLVDLGLPQFAGPGSLFARDPEIKREAVFAGPRMRPSATLVQRGRDYAEVASESGRG
                     FTELTRALPAFRSVLAEYAGGLAELAAVRDRLRGWRFYDGFRVDAKAPARQPRVGTRT
                     QVLSDDGGDLAAAIQTIVEAGFEDFERAVADAFDGATVSIAVTDGVFDLQLQQRGMLR
                     PLRAAELSDGTLRFLLWGAALLTPSPPSLMVLNEPETSLHPDLVAPLAAMIAAAARTA
                     QVLVVTHSRTLREHLAGAHASELELCKEWGETRVVGQTMLSAPPWEWGKR"
     gene            complement(1980..3020)
                     /locus_tag="VGP27_03375"
     CDS             complement(1980..3020)
                     /locus_tag="VGP27_03375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV7850392.1"
                     /translation="MTQWILHVDLDQFQAAVEVRRNRHLQGLPIVVGGNGDPSEPRKV
                     VTCASYPAREFGVRAGMPLRTAARKCPDAVFLPLDTDAYDEASDEVMGLLRDLGHPVE
                     VWGWDEAYLGAEVDDPIKLAEQIREVVAAGTGLSCSVGISDNKQRAKVATGFGKPAGT
                     YRLTESNWMTVMGDREVDALWGVGPKTAKTLAALGITTVADLAATDATVLTSTFGPTT
                     GLWILLLAKGGGDTTVSAQPWVPRSRSHVITFPEDLTDRAEIDAAVVDLARQTLAEVS
                     DQGRVVTRVAVTVRTKTFFTRTKIRKLVTAGTDAELITVTALQLLDQFELDRPVRLLG
                     VRLELAPPAGGY"
     gene            complement(3017..>3553)
                     /locus_tag="VGP27_03380"
     CDS             complement(3017..>3553)
                     /locus_tag="VGP27_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850393.1"
                     /translation="RNRTLLLEAARRLIAERGADAVSMDDIAAAAGVGKGTVFRRFGS
                     RAGLMMVLLDEDERASQEAFLFGPPPLGPDAPPLERLLAFGRERLCFAHTHHSLLSSA
                     NRDPQSRYGAPAMVLRTHVRLLLATARSTGDLDAQTDALLALLDADYVHHQLTERGET
                     LESLGDKWESLARKLCGA"
BASE COUNT          562 a         1191 c         1218 g          582 t
ORIGIN      
        1 cgccacccat cgtgaggtcg ggccgaaccg ccagaagctg ctcggtcacc gagccaagac
       61 cgccgttttc gatcgtttca gtcgcgcatt tcttcgccgt ctcttcgggg ccgtagcact
      121 tccgctcact gatgtgagag atcagtgccg cgggcgtggc atcctgcacc tctgatgtgg
      181 tgatgtctcc ggtcgcgaag ccgtgctcct tggcgagttc caggatcgtc ttctgcggat
      241 cgcctttgat gttgactgcg agcgcaccgt cgtaagtctt ggtggcggtc gtccagccgc
      301 tggcgctgga ggcggagtcg gcgacgtagt cgggcttgcc gtccttggtg agcgcatatg
      361 tggtgtagga gcccgtcagt gggagggcgt cgatgccgtc gaagaagccg ccggcgccct
      421 cggcgtagtc gcgggcaagg gtgatctcgg aatcacccat gccgtcgccg atcagcagga
      481 tgacgttgcg ggaggccgag ccgctgatgc tgtcgcggat ggtgccggtc tggtcgacgc
      541 cgagccgggc ggcgccgccg gtggtcgata aatcgccctt gggggcgcga ctgagcggat
      601 cgccgatcga ctccttctcc tcgtcgctgc cgcaaccagc aagaacgagg acggcgacgg
      661 cgcccagcag acaggcggtt ttggacatgt ggttcaccgg catgaggctc ctagctcggg
      721 atcggcccag cgaagtgaac ggtatcgcgt gaaaagagat tgccggggag gctaatgtcc
      781 aattcgctca gcactcttca ccgcttgccc cattcccatg gcggcgcgct gagcatggtc
      841 tgaccgacga cgcgcgtctc gccccactcc ttgcacagtt cgagttccga cgcgtgggcg
      901 ccggccagat gctcgcgcag cgtccgcgaa tgggtcacca ccagcacctg ggcggtccgc
      961 gccgccgcag cgatcatggc ggccaacggc gccaccagat ccggatgcaa cgacgtctct
     1021 ggctcgttga gcaccatcag cgacggcggg ctcggggtca gcagcgcagc accccacagc
     1081 aggaatcgca gtgtcccatc agataattcg gcggcccgca aaggccgcaa catgccccgc
     1141 tgctgcaatt gcagatcgaa gacgccgtcg gtgacggcga tcgacaccgt ggcgccgtca
     1201 aacgcgtcgg caacggcgcg ttcgaaatct tcgaagccgg cctcgacgat cgtctgtatc
     1261 gctgcggcca ggtcgccgcc gtcgtccgac agcacctgtg tgcgggttcc gacccgcggt
     1321 tgccgggcgg gagccttggc gtcgacccgg aaaccgtcgt aaaaccgcca gccgcgcaag
     1381 cggtcccgga cggcggccag ttcggccagc ccgcccgcgt actcggcgag cacgctgcga
     1441 aaggcgggca acgcgcgggt cagttcggtg aacccgcggc cggactccga cgccacttcg
     1501 gcgtagtccc tgccacgctg gacgagcgtc gcactcggcc gcattcgcgg acctgcgaac
     1561 acggcttccc gcttgatctc tggatcgcgg gcgaacagcg accccgggcc tgcgaactgc
     1621 ggcaggccca gatcgacgag atagccgaag tcgtcggcgg cgaatccgag ttcgagcgcc
     1681 accggccggg tacgcgtggt gccctcggtg gcgcccgtgc ggcgtgcgcc gccggtggtt
     1741 tcagggccgg cccacaacac cgactcgagt ccgccctcgc gggccagcga tccgatcacc
     1801 tcgccgcggc cgcagtcggc cagcagtcgc agtgcgcgat acaccgacga cttaccggtg
     1861 ccattggccc cggtgaccac agtgaggccg gtcagcggca cgacgatgtc gcgcagcgag
     1921 cggtagccgc ggatcgcgac ggtgttgagc atgtgccgag ggtattgccc cggtatgact
     1981 cagtagccgc ccgccggcgg ggcaagctcc agccgcacac cgagcagccg aaccgggcgg
     2041 tcgagctcga actggtcgag cagctgcagt gccgtcaccg tgatgagctc cgcgtccgtg
     2101 cccgcggtga cgagcttgcg gatcttggtg cgggtgaaga acgtcttggt gcgtaccgtc
     2161 acggccaccc gggtgacgac gcgaccctgg tcgctgacct cggcaagggt ctgccgggcg
     2221 agatccacca cggccgcgtc gatttcggcg cgatcggtca agtcttcggg gaaggtgatc
     2281 acgtggctgc gcgagcgcgg cacccacggt tgcgcgctga cggtggtgtc gccaccaccc
     2341 ttggcgagta gcagtatcca cagcccggtg gtcggcccga acgtcgacgt cagcaccgtg
     2401 gcgtcggtgg cggcgaggtc agcgacggtg gtgataccca gcgcggcaag cgttttggcg
     2461 gttttgggcc cgacgcccca cagggcgtcc acctcgcggt cacccatgac cgtcatccaa
     2521 ttggactcgg tcagccgata cgtacccgcg ggcttgccga acccggtggc gaccttggcg
     2581 cgttgcttgt tgtcgctgat gccgaccgaa cacgacagac cggtgcctgc cgcgaccacc
     2641 tcgcggatct gctccgccag cttgatcggg tcgtcgacct ccgcacccag gtaggcctcg
     2701 tcccaacccc agacctcgac cgggtggccg agatcgcgca gcaatcccat cacctcgtcg
     2761 gaggcttcgt cataggcgtc ggtgtccaac ggcagaaata ctgcgtcggg gcatttgcgt
     2821 gccgccgtgc gcagtggcat accggcgcgc acgccgaact cgcgtgccgg atatgacgcg
     2881 cacgtcacga cttttcgcgg ttcgctggga tcgccgttgc cgccgacgac gatcggcaag
     2941 ccctggaggt gacggttgcg acgtacttcg accgcagcct gaaactggtc gagatcgacg
     3001 tgcagtatcc actgggtcat gcgccgcaga gcttgcgcgc caagctctcc cacttgtcgc
     3061 cgagcgattc cagcgtctcg ccgcgttcgg tgagctggtg atgcacatag tcggcgtcca
     3121 gcagcgccag cagcgcgtcg gtctgtgcgt cgaggtctcc agtgctgcgg gcggtggcga
     3181 gcagaaggcg cacgtgggtg cgcaacacca tcgccggggc gccgtagcgg ctctgcggat
     3241 cccggttggc cgacgagagc agcgagtgat gggtgtgggc gaaacacagc cgttcgcgcc
     3301 cgaaggccag cagccgttcc agtggcggtg cgtcaggccc gagtggaggg ggtccgaaga
     3361 ggaacgcctc ctggctggcc cgctcgtcct cgtcgagcag caccatcatc agcccggccc
     3421 ggctgccgaa ccggcggaac accgtgccct tgccgactcc ggcggcggcg gcgatatcgt
     3481 ccatcgacac cgcatccgca ccacgttcgg cgatgagccg gcgggcggcc tcgaggagca
     3541 acgtgcggtt gcg
//
LOCUS       DASYLX010000143         8922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_993933,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000143 DASYLX010000000
VERSION     DASYLX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993933"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGP27_03385"
     CDS             <1..1033
                     /locus_tag="VGP27_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7850394.1"
                     /translation="VDWKDIQFGFGGSHEVSNPDAVTRLVGLTGITFTNVFNACATAA
                     SAIQQTADTIRLGKYDIGIAIGLDKHPRGAFTDDPAKLALPQWYAENGQFVTTKFFGM
                     KANHYLHKHGISQETLAKVAAKNFRNGEKNPNAFRRKPISEEEILNSAVLNYPLTQYM
                     FCAPDEGAAAVIMCRGDIAHRFTKKPVYVKATEIRTRTFGAYEVHATFAPLDEDPSPT
                     VYAAKAAYEAAGIGPDDVDVAQLQDTDAGAEVIHMAETGLCADGEQEKLLADGATEIG
                     GSMPVNTDGGLIANGEPIGASGLRQVHELVRQLRGEAGDRQVPGEPKVGLAQVYGAPG
                     TASATILSV"
     gene            1058..1414
                     /locus_tag="VGP27_03390"
     CDS             1058..1414
                     /locus_tag="VGP27_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850395.1"
                     /translation="MPSVADTVNRYVELLATGSADELAALYTQNATVEDPVGSGVRHG
                     RDEIRELYDRIEKQDRTVELLAVHVNGNEASFLARLTVIAGDSRTRIDGIDVMTFDDR
                     ARITSMRAFWSAPDGR"
     gene            1481..1651
                     /locus_tag="VGP27_03395"
     CDS             1481..1651
                     /locus_tag="VGP27_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850396.1"
                     /translation="MQILAEAEDLPTTANIKTKLISHTRLPCGEPRRSQSHTHRNELA
                     TRPTAGDRSAAA"
     gene            complement(1714..2217)
                     /locus_tag="VGP27_03400"
     CDS             complement(1714..2217)
                     /locus_tag="VGP27_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7850397.1"
                     /translation="MSAYQTLVVGTDGSDTSLRAVDHAGAFAAALNAKLIIAMAHLPN
                     GDKGGWGRPARPDRVIDGRAEVLLRGEGDFKMHGMSPIYGILRDARDRARAAGATNIE
                     TRPIQVGRLGGPAQALSRLAEEVKADLLVVGNVGLGTRTDKWLGSVPGDVLRRAKTHV
                     LVIHTSD"
     gene            complement(2295..2630)
                     /locus_tag="VGP27_03405"
     CDS             complement(2295..2630)
                     /locus_tag="VGP27_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7850398.1"
                     /translation="MMFARHDVPKLFGAAIAVAALGLVTPGSAAADATDDAFLKKLFA
                     DAIDFSQPEQAIPRARTVCEAFASGKSPANVHEMTLDHSAFSPRQAAIFMADAVQAYC
                     PGYANQFMS"
     gene            complement(2793..3119)
                     /locus_tag="VGP27_03410"
     CDS             complement(2793..3119)
                     /locus_tag="VGP27_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850399.1"
                     /translation="MNLTRLAVMFVGLLFVNGVLAGTASATTVVECRMQVAHIRNGTV
                     EAQRQSAQTKDQSGSIAKLDDISLMLAEGKNADAIQKLTDFVAQGYPSIGVPDVISCI
                     NAIGTP"
     gene            3309..3764
                     /locus_tag="VGP27_03415"
     CDS             3309..3764
                     /locus_tag="VGP27_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850400.1"
                     /translation="MSFPVDPLRAKPSLGVLSATALTAACLSFGTATASADDPPPPPL
                     HQIHYTVWAEQPFTAEIYYRDTDPPNWAEYSHNPYLFSPNIEVPVGPEQQWNLDVGLV
                     DPDQWAMVVAGTGGESPKKPNIHCALAVDGVVVKTNQGPKGALCSIRAW"
     gene            complement(3722..4378)
                     /locus_tag="VGP27_03420"
     CDS             complement(3722..4378)
                     /locus_tag="VGP27_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850401.1"
                     /translation="MASARRIGAPDAKNRGVLLDAAEELLLQEGYAAVTSRRVADAAG
                     LKPQLVHYYFRTMEDLFLEVFRRRAEEGLGVLATALASPQPLWALWRFSTQPEATRLT
                     MEFMGLANHRKALRAEIVYYAERFRKEQNKAIAAALRRYNVDVAEVPPVVWTVFATSV
                     SQALVMERALGMSTGHAETFKYCEQWLRRLEGEPVTVANAAPGSDHQARIEQSAPFGP
                     "
     gene            4568..5848
                     /locus_tag="VGP27_03425"
     CDS             4568..5848
                     /locus_tag="VGP27_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850402.1"
                     /translation="MTTDYDSVDYFTDPSLVPDPHPYFDHLRTKDASCCPINNGVLAV
                     TGWEAANAVYKDTENYSSCVAVAGPFTPIPFVPQGDDICAQLEKHRAEIPMYEHMVTM
                     DPPYHTDARSLLSRLLTPKRLKENEDFMWRLADRHIDEFITDGKCEFLAAYARPFSLL
                     VVADLLGVPEEDHEEFREAFGAQRPGSTIGGLDHEPIAANPLEWADEKFSHYIEDRRQ
                     NPRDDVLTGLATALYPDGSTPEVVDVVRTATFLFGAGQETTAKLLSAALRVLGDQPKI
                     QQQLRDDRSLIPVFIEECLRMDSPVKSVFRMARKSTTLGETAVPAGTTVMVSPGAANR
                     DPKRFEHPHEFQLDRKNVREHIAFSRGIHSCPGAPLARVEGRVSMERILDRLSDITID
                     DEKHGPADSRAYNYEPTFILRGLTEINIRFTPAG"
     gene            5967..6353
                     /locus_tag="VGP27_03430"
     CDS             5967..6353
                     /locus_tag="VGP27_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850403.1"
                     /translation="MRKFIAAPVVACGLLSATIPFAGEASAFCDSADCVPNVARNVAE
                     GAPCAPGPSYVFGLDSDRNTLICAAQGMWVRVGPLIGTRELALPCERPGDSAQQPDTT
                     NDLQAHTPGVPLKCAEINAAVRWIHF"
     gene            complement(6375..6755)
                     /locus_tag="VGP27_03435"
     CDS             complement(6375..6755)
                     /locus_tag="VGP27_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850404.1"
                     /translation="MRSVFAVLATLLTTGLLPGAPPAAADPPECDYPSCTPGIRPNVV
                     LGSYCDNPTYYAFGVTSWGRLVFCGSPRRYEPRWFRSPEMHGIRNESAPCPAQDGEVA
                     QAPDGLFLTCVANNGASLWARGDT"
     gene            6844..7371
                     /locus_tag="VGP27_03440"
     CDS             6844..7371
                     /locus_tag="VGP27_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850405.1"
                     /translation="MGVAAAATAIAVIGSLVTAPSGQATDYGVALNGPYRAFSNGEWA
                     QTSAGPNGAGGAMVYIDQPSKMETWTVTSDCISPIQCTGEVRSDAGWTGTLKFNGDSW
                     RVDRDIPNWQPCPDGTAATGHQNFALWGFDPTTSEKNKNRDLVVGWERTMAPSGSCGY
                     NKPVVTQLPVRLEKI"
     gene            complement(7386..>8922)
                     /locus_tag="VGP27_03445"
     CDS             complement(7386..>8922)
                     /locus_tag="VGP27_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7850406.1"
                     /translation="VTYRGVQIGEVREVKLTKDGAEAKLTLDTKPKIPADLQADVRSV
                     SAVGEQYVDLRPRTDSGPYLRDGSVITKDNTTIPQQVGPMLDQVSKLVDSIPGNRLSD
                     LLDESFKGLNGAGEDFGSLLDSSANITNHLNDVSDQSRALIDDSGPLLDSQAETTDAI
                     RTWARSLAGVTEQVAQDDPKVRAILQRGPGFAQEVSQLLTQVKPTLPILLANLTTLGQ
                     ILVTYNPSLEQLLVLLPGVIAAQQSFGLPKNNPMGLPLSDFSFTIADPNACTVGFLPP
                     SQWRNPADTTTVDTPDGLYCKLPQDSPISVRGARNYPCMGHPGKRAPTVELCDDPKGF
                     VPTAIRQHTLGPNPFDPNLVAQGVPIDDRVDFNDRIYAPIEGTPLPPGAVPAGTPPIA
                     PNPLTAPLPPAPSAPAPPGNSLNGVPIPPVEAPLAPAAGEVPPAPQGPPPGPVEVPPS
                     DGALPAAPSAFNGNASGGPSVAITSYDPHTGRYMSPDGQLQQVSTPVAGTQLKSWKDM
                     LPI"
BASE COUNT         1679 a         2912 c         2800 g         1531 t
ORIGIN      
        1 tgtcgactgg aaggacattc agttcggctt cggcggcagc catgaagtgt cgaaccccga
       61 cgcggtgacc cgactggtcg gcctcaccgg gatcacgttc accaacgtgt tcaacgcctg
      121 cgcgaccgcg gcgtcggcca ttcagcagac cgccgacacc atccggctcg gcaagtacga
      181 catcggcatc gccatcggtt tggacaagca tccgcgggga gcgttcaccg acgacccggc
      241 caagctggca ctgccccagt ggtacgccga gaacggccag ttcgtcacca ccaagttctt
      301 cggcatgaag gccaaccatt acctccacaa gcacggcatc tcgcaggaga cgctggccaa
      361 ggtcgcggca aagaacttcc gcaacggtga gaagaacccg aacgcgttcc gccgcaagcc
      421 gatttccgaa gaggagatcc tcaactcggc cgtgctgaac tatccgctca cgcagtacat
      481 gttctgcgca cccgacgagg gcgcggctgc ggtgatcatg tgccgcggcg acatcgcgca
      541 ccgcttcacc aagaagccgg tgtacgtcaa ggccaccgag atccggacgc gcaccttcgg
      601 tgcctatgag gtgcacgcga ccttcgcgcc gctcgacgag gacccatcac caaccgtcta
      661 cgccgcgaag gccgcgtacg aggcggcggg catcggtccc gacgacgtcg acgtggcgca
      721 gctgcaggac accgacgcgg gcgccgaggt catccacatg gccgagacgg ggctgtgcgc
      781 cgacggcgag caggagaagc tgctggccga cggcgcaacc gagatcggtg gctcgatgcc
      841 cgtcaacacc gatggcgggc tgatcgccaa cggcgagccg atcggtgcgt ccgggttgcg
      901 gcaggtgcac gaactggtgc gccagttgcg tggcgaggcg ggcgaccgcc aggtgcccgg
      961 cgaaccgaag gtgggcctgg cgcaggtcta cggcgcgccc ggtacggcgt cggcgacgat
     1021 cctgtcggtc tgactgaggc atcgatcggc cgagacagtg ccgagcgtgg ccgacaccgt
     1081 caaccgatat gtcgagctgc tcgccacggg cagcgccgac gaactggctg cgctctacac
     1141 gcagaatgcc acggtggagg atccggtcgg cagcggtgtg cggcacggtc gcgacgaaat
     1201 tcgcgagctg tacgaccgca tcgaaaagca ggaccgcaca gtcgaactcc tggcggtaca
     1261 cgtcaacggc aacgaggcgt cgttcctggc gcggctgacc gtcatcgcgg gcgacagccg
     1321 gaccaggatc gacgggatcg acgtgatgac attcgacgac cgggcccgga tcacctcgat
     1381 gcgggcgttc tggtccgcgc ctgacggtcg ttgatcgttg cttgccgacg tctactgtcg
     1441 gtggatagcc gatcggcgag ctaaccccga aggagagtcg atgcagatcc ttgccgaagc
     1501 cgaagacctg ccgacgacgg cgaacatcaa gaccaaactg atcagccaca cccgacttcc
     1561 ttgcggcgag ccccgacgca gccaatccca tacccaccgc aacgaactgg ctacccgtcc
     1621 tacggccggg gaccggtctg ccgctgcgta actttgacac ccttcgcccc cgcgaggtcc
     1681 ggatggacag acggcgtcga acctggagct tcttcagtcg ctggtgtgga tgaccagcac
     1741 gtgggtcttg gccctgcgca agacgtcgcc gggcaccgag ccaagccact tgtcggtgcg
     1801 tgtgcccagt cccacgttgc caaccaccaa caaatcggcc ttgacctcct cggcgaggcg
     1861 ggacagcgcc tgtgccggac cacccagacg acccacctga atcggtcgcg tctcgatatt
     1921 cgtcgcccca gccgccctgg cccggtcgcg ggcgtcccga aggatcccat atatggggga
     1981 catcccgtgc atcttgaagt ccccctcgcc ccggaggaga acctcggcgc ggccatcaat
     2041 cacgcgatcc ggccgggccg gtcttcccca gcctcccttg tccccgttcg gcaagtgtgc
     2101 catcgcgatg atcaacttcg cgttaagtgc cgcggcaaac gcacctgcgt ggtccaccgc
     2161 acgcaacgac gtgtccgagc catcggtgcc gaccaccagg gtctgatagg cgctcatttc
     2221 ccctccagga taaagcccga tggcggccca acctctcgaa aaaggcgaat aggagcctta
     2281 ccaaaaacct ttagtcagct catgaactgg ttggcatacc cggggcagta ggcctgcacc
     2341 gcgtcggcca tgaaaatcgc ggcctgacgt ggggagaatg cagagtggtc aagtgtcatc
     2401 tcgtgcacat tcgccggcga cttgccggag gcaaacgcct cgcagaccgt cctggccctc
     2461 ggaatagcct gttccggttg tgagaaatcg atcgcgtcgg cgaacagctt tttcaggaac
     2521 gcgtcatcgg tggcgtcggc ggcggcgctg ccgggggtga ccaaacccag agcggccacc
     2581 gctatcgccg cgccgaaaag cttggggacg tcgtggcggg cgaacatcat gatgaccact
     2641 cctaagtcga accgcaacgc gacaactcta gcctcacgct gagcaaccgt gttcccacta
     2701 ccaggaactg cgaaacggag ttgtggccgg gccggcccct actgctgcga acgattgcat
     2761 cggaggattg aaagctgttg cccgcagtcg agctaggggg tgccgatcgc gttgatgcag
     2821 ctgattacgt ccggcacgcc gatggagggg tatccctgcg cgacgaagtc ggtcagcttc
     2881 tgtatggcat cggcgttctt gccttccgcc agcatcaggg agatgtcgtc gagctttgcg
     2941 atgctgccgc tttggtcctt cgtctgcgcg gactggcgct gggcttcgac cgtaccgttc
     3001 cgaatatgcg ctacctgcat ccggcactct acgacggtcg tcgccgaagc ggtgccggcc
     3061 aagaccccgt tcacgaacag caaccctaca aacatcacgg ccaaccgggt caggttcatg
     3121 ggaaacccct ctgcgcaatg atgctgcccc cgccaggcag gcaaaccgca gccaaccact
     3181 ggcgttgata ccccgcgcgg tcttatgaca aacccggtgc cgggtaccgg ctggcgaatc
     3241 cagaactcgt accaagtaga tcgccgattc cagaccggct gtggaaacaa cgttttagga
     3301 tatcggaaat gagctttccg gttgatcccc tgagggccaa gccgtcattg ggcgtgctgt
     3361 cggcaacggc gttgacggcg gcttgtctct ccttcggcac cgcaaccgcg tcggcggacg
     3421 acccgccgcc tccgccgctg caccagatcc attacacggt ttgggccgaa cagccgttca
     3481 ccgccgagat ctactatcga gacaccgacc cgccgaactg ggcagagtac agccacaacc
     3541 cctacctgtt cagccccaac atcgaagtgc ccgtcggtcc ggagcagcaa tggaacctcg
     3601 acgtcggttt ggtcgacccc gatcagtggg cgatggttgt cgccggcact ggtggcgagt
     3661 caccgaagaa acccaacatc cactgtgcgc ttgccgtcga cggcgtggtc gtgaagacca
     3721 atcagggacc gaagggtgcg ctctgttcga ttcgagcctg gtgatcgctc cccggcgccg
     3781 cgttggctac ggtcaccggc tccccttcta atcgacgcag ccattgctcg caatacttga
     3841 acgtctcggc gtgaccggtg gacatgccga gagcccgctc catcaccagc gcctgcgaca
     3901 cgctcgtcgc gaacaccgtc cataccaccg gcggtacctc ggcgacgtcc acgttgtagc
     3961 gccgtagcgc agcggcgatc gccttgttct gctccttccg gaaccgttcg gcgtaataga
     4021 cgatttcggc ccgaagcgcc ttgcgatgat tcgccagccc catgaactcc atcgtcagcc
     4081 gggtggcctc aggctgcgtg ctgaacctcc acagggccca cagcggctga ggcgacgcga
     4141 gtgccgtcgc cagcaccccg aggccctctt cggctctgcg gcggaacacc tcgagaaaga
     4201 gatcttccat cgtcctgaag tagtaatgca caagttgcgg cttgagtccg gcggcatccg
     4261 cgacacggcg agatgtgacg gcggcgtagc cctcctgcag gagcagctcc tcggcagcgt
     4321 cgagcagcac gccgcgattc ttcgcatccg gcgccccaat tcttcgggcc gacgccatgt
     4381 cacctccccg aatcttcctc ccggacggcc gctactggag acgttagccg tcattccggg
     4441 tgtcatcttg accacgactc taccgccatg ctaggcaggt gctcagcatt tgtgtgccgt
     4501 gcacgttcag cgcggtgcga cgtccagcca atggagctgt cacgggttcc aggaggaagc
     4561 gctcttcatg accaccgatt acgactccgt cgactacttc accgacccgt ctctcgttcc
     4621 agacccgcat ccctatttcg accatctacg gaccaaggac gcatcctgct gcccgatcaa
     4681 caacggggtg ctcgccgtca ccggctggga ggccgccaac gccgtctaca aggacaccga
     4741 gaactactcg tcctgcgtcg cggtcgcggg cccgttcacc ccgatcccgt tcgtgcccca
     4801 gggcgacgac atctgcgcgc agttggagaa gcatcgcgcc gagatcccca tgtacgagca
     4861 catggtgacg atggaccccc cgtaccacac cgatgcgcgg tcgctgctga gccggttgtt
     4921 gacgcccaag cggctgaagg agaacgagga cttcatgtgg cgtctggccg accgccacat
     4981 cgacgagttc atcaccgacg gcaagtgcga gttcctggcg gcctacgcca ggccgttctc
     5041 actgctggtg gtggccgatc tgctcggcgt ccccgaagag gaccacgagg agtttcgcga
     5101 agcattcggc gcccagcgcc ccggcagcac tatcggaggc ctcgaccacg agccgatcgc
     5161 ggccaacccc ttggagtggg ccgacgagaa gttcagtcac tacatcgagg atcgcaggca
     5221 gaacccgcgc gacgacgtgc tgaccgggct ggccactgcg ctctaccccg acggctcgac
     5281 acccgaagtc gtcgacgtcg tgcgcaccgc cacgttcctg ttcggggccg gccaggagac
     5341 caccgccaag ctgctcagtg cggcgctgcg ggtgctcggt gaccagccca agatccagca
     5401 gcagctgcgc gacgaccgca gcctcattcc ggtgttcatc gaggagtgcc tgcggatgga
     5461 cagcccggtg aagagcgtgt tccggatggc ccgcaagtca acgactttgg gcgaaaccgc
     5521 cgtcccggcg ggtaccacgg tgatggtcag cccgggcgcc gcgaaccgcg atccgaagcg
     5581 gttcgagcac ccgcacgagt tccagcttga ccggaaaaac gtccgcgagc acatcgcgtt
     5641 cagccgcggt atccactctt gccccggcgc gccgctcgcc cgagtggagg gccgggtcag
     5701 catggagcgg attctcgacc ggctgagcga tatcacgatc gacgacgaga agcacggtcc
     5761 cgccgactcc cgcgcataca actacgaacc aacgttcatt ctgcgcggcc tcaccgaaat
     5821 caacatcagg ttcacacccg ccgggtagcc gtgacaggcg cgcaacatcg tccggtccgc
     5881 cgactgagaa cactgctcgc agctgcggag acaacgctag gctggactgg ctggcgggct
     5941 cggtctgatc tccgaaggag gcagaggtgc gcaagttcat cgcggcgccg gtcgttgctt
     6001 gtggattact ctcggcgaca atacctttcg ccggtgaggc atcggcgttt tgtgactcgg
     6061 cagactgcgt gccgaacgtt gcccgcaatg tcgccgaggg cgcgccctgc gcacccggtc
     6121 cttcttacgt ctttggcctg gattccgacc ggaacaccct catctgcgcc gcgcagggca
     6181 tgtgggttcg ggtcggccca ctgatcggca cgcgcgagtt ggcgctgccc tgcgaaaggc
     6241 cgggcgatag cgcgcagcaa cccgacacaa ctaatgactt acaggcgcat acccccggcg
     6301 tcccgctgaa gtgtgcggag atcaacgcgg ccgtacggtg gatacatttc taacacccct
     6361 ggccggcggg cgatctaggt gtccccgcgc gcccacaggc tggcgccgtt attggccacg
     6421 catgtcaaga acagcccgtc gggcgcctgg gccacttcac cgtcctgggc ggggcagggt
     6481 gcgctttcgt tcctgattcc atgcatctcg ggcgagcgga accaccgcgg ttcgtatctc
     6541 ctgggtgagc cgcagaacac cagccgtccc cacgaagtca caccgaacgc gtagtaagtg
     6601 gggttgtcgc agtacgagcc gagcacgaca ttgggtctga tgcccggtgt gcacgatggg
     6661 tagtcgcact cgggcggatc ggcggccgcc ggcggcgcgc ccggcaacag accggtggtg
     6721 agcaaggtag cgagcaccgc gaagactgat cgcacagcaa cattttcaca taggcgggaa
     6781 cgaagctctc gctattggag aagatagctc cagaccgcgt taggctactg ggttatgcgc
     6841 gcaatgggtg tggctgcggc cgcgacggcg atcgccgtga tcggaagtct ggttacagct
     6901 ccgtccgggc aggccacgga ctacggagtt gcgctcaatg gcccctatcg ggccttctcg
     6961 aacggcgaat gggctcaaac cagcgcgggg ccgaacggcg cgggtggagc catggtctac
     7021 atagaccagc cgtcgaagat ggagacctgg actgtgacgt cggactgcat cagtccgatc
     7081 caatgcaccg gcgaggtcag aagcgacgcc ggctggaccg gtacgttgaa attcaacggc
     7141 gattcttgga gggtcgaccg cgacatcccc aactggcagc cgtgccccga cggcaccgca
     7201 gccaccggtc atcagaactt cgctttgtgg gggttcgacc cgaccacgtc tgaaaagaac
     7261 aagaaccggg acctcgtcgt gggctgggaa aggacgatgg ccccgagcgg ctcatgcggt
     7321 tacaacaagc cggttgtcac ccagttgccg gtgcgactgg aaaaaattta gcgaccgctg
     7381 gcccatcaga tcggcagcat gtccttccac gatttgagtt gcgtcccggc aaccggagtc
     7441 gacacctgct gaagctgccc atcggggctc atataacgcc ccgtgtgcgg atcgtagctt
     7501 gtgatcgcaa ccgatggacc accagacgcg ttgccgttga acgcacttgg agccgcgggt
     7561 agtgcgccgt ctgacggtgg cacctcgacg ggcccaggcg gcggaccctg cggcgcaggc
     7621 ggtacctctc cagctgccgg cgcgagcgga gcctcgacag gcggtatcgg caccccattg
     7681 agcgaattgc ccggcggcgc aggcgccgac ggtgcgggcg gcagtggagc ggtgagtgga
     7741 ttgggtgcga tcggtggcgt tccggccggg acggcaccag gcggcagggg ggtaccctct
     7801 atcggcgcgt agattcgatc gttgaagtca acgcgatcat cgatcggaac tccttgcgca
     7861 accagattcg ggtcgaacgg gttgggtccg agcgtgtgtt ggcgaattgc agtcggcaca
     7921 aaaccctttg ggtcgtcgca gagttcgacc gtgggagcgc gctttccagg gtgccccatg
     7981 cacggatagt tgcgtgcgcc gcgcaccgag atcggcgaat cctgcggcag tttgcagtac
     8041 agcccgtccg gtgtgtcgac ggtggtggta tcggcagggt tgcgccactg cgatggaggt
     8101 aggaagccga ccgtacacgc gttcggatca gcgatagtga atgagaaatc actcaggggc
     8161 agacccatcg ggttgttctt tggaagcccg aatgactgct gagccgcgat gaccccgggc
     8221 agaagcacca gaagttgttc gagcgacggg ttgtacgtga cgaggatctg acccaaggtg
     8281 gtgaggttcg ccaacaagat cggcagcgtg ggcttcactt gagtgagaag ctgtgacact
     8341 tcctgcgcga agccgggtcc gcgctgcagg atcgcccgca ctttcggatc atcctgcgcg
     8401 acctgctcgg tgactcctgc caagctgcgc gcccacgtcc ggatcgcgtc cgtggtctcg
     8461 gcctgggaat ccaaaagcgg cccactgtca tcgatcaacg ctcggctctg gtcagatacg
     8521 tcgttgaggt gattggtgat gttggccgac gagtcgagca gggaaccgaa gtcctcgccg
     8581 gcgccgttca accccttgaa cgactcatcg agcaaatcac tcagcctatt tccgggaatg
     8641 ctgtccacaa gcttgctcac ttgatccagc atcgggccga cctgctgcgg aattgtggtg
     8701 ttgtccttgg tgatcaccga gccatcacgc aagtacgggc cggaatcggt acgggggcgt
     8761 aagtcgacgt actgctcgcc gacagccgag acgctgcgaa cgtcggcctg cagatccgcg
     8821 gggatcttag gcttggtgtc caacgtcagc ttggcttctg cgccgtcctt ggtgagcttc
     8881 acctccctca cctcacctat ctgaacgccc cggtaagtca ca
//
LOCUS       DASYLX010000144         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2639236,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000144 DASYLX010000000
VERSION     DASYLX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639236"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGP27_03450"
     CDS             <1..493
                     /locus_tag="VGP27_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850407.1"
                     /translation="LREDVTGTLQAAVADPDVAGRLGHLAKAEQWSGFGDFGSATAVF
                     TTARGGTARSTTKQARQEHPPQPRRADEHASARRTEEARKALAAAERAKDDAANQLST
                     RQTDLAVARLRRDEMRQRLEEAERNLDAAEKAYSEAKQAGRDAADLVREAKARLKQSE
                     RTR"
     gene            complement(495..869)
                     /locus_tag="VGP27_03455"
     CDS             complement(495..869)
                     /locus_tag="VGP27_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEV7850408.1"
                     /translation="MATDLTLYLDDQPGELARLGDVLGKAGANIAGLCAVASGGGQAE
                     VHILIDDATAAFEALHDAGIRIAAEQEVLVLDVEDRPGALGEVACELGTARVNLTLVY
                     LATNTRLVLGADNLAEARAAVR"
     gene            1146..1436
                     /locus_tag="VGP27_03460"
     CDS             1146..1436
                     /locus_tag="VGP27_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7850409.1"
                     /translation="MRRALLIIAAVLAVFVSGCGSKINPDGAAKSVVDIVSRQTKFKP
                     KDVSCPSGVDAVVGAEFDCKFTGPEGTEYTAHLKVTKVEGDNVEFYVQSKPS"
     gene            complement(1485..2156)
                     /locus_tag="VGP27_03465"
     CDS             complement(1485..2156)
                     /locus_tag="VGP27_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEV7850410.1"
                     /translation="MFAVATASLLTVVQQLSGTPYISGGDSAAGTDCSGLVSFVTNAA
                     SGRPIYGDRFNTGNIEGALKARGFQYGSKPGALNVGWNRGHTAATLPDGTPVSSGEGG
                     GVRVGGGGAYQTQFSRHMYLEMAPDAPAPEEQLPPGVMPPPPNAPLPPEGVIVPVGME
                     APLPPPAGAPLPPPPPGADLLPPPPGAELPPPPPGAELPPPPLPGAELPPPPPGAPEP
                     PPIVA"
     gene            2546..2776
                     /locus_tag="VGP27_03470"
                     /pseudo
     CDS             2546..2776
                     /locus_tag="VGP27_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(2854..3990)
                     /locus_tag="VGP27_03475"
     CDS             complement(2854..3990)
                     /locus_tag="VGP27_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7850411.1"
                     /translation="MDLPQPDPHTPHLADVVPSVLAAMGVAEFDGRIDLAAEVRGACV
                     LLIDGLGADLLDAYADDAPVMAALRGRTLQAGFPSTTVAGLAAIGTGCRSGEHGLVGL
                     SFRLPGVGLINPLRWRPHPRGDDLRDTVPPEQVQPMATTFERAVSAGVAVSVVSGAEF
                     TGSGLTRAVLRGGRYVGVRALGDLAEGVRAAVADGGFCYGYHGEVDLLGHLYGPGSAA
                     WRMQLREVDRLVESVVEGLPSGGLLAIVADHGMVTVDDDDAVDIDSRAALLDGVQAVG
                     GEPRARHVYVDHGAADSVLAAWRETLADGAWVASRDEAIAAGWFGGSVRDDVRMRIGD
                     IVAAARGSATMVRRTVEPMESSLIGHHGSLTSAEQRIPLLLAYG"
     gene            4019..4603
                     /locus_tag="VGP27_03480"
     CDS             4019..4603
                     /locus_tag="VGP27_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV7850412.1"
                     /translation="MPLRIRTVAGIGLTLLLGACTWSQAAPAAQLTNGPVPDDGTFSI
                     GPAATDSIGGYLPDGQTLSPFDATNPVIGWLDPPLLAAVQNAARSAKDDGVDVRIASG
                     WRTKGFQQRLFDDGVRTYGSVEAAQQFVASPDTSQHVLGKAVDVSPVEADTWMIRNGS
                     RFGLCQIYANEIWHFELAADKDGACPPLRPNAAG"
     gene            complement(4610..>5042)
                     /locus_tag="VGP27_03485"
     CDS             complement(4610..>5042)
                     /locus_tag="VGP27_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV7850413.1"
                     /translation="VDSLTLVGWSMGGLAAAGLTVHASHHDVRLRHTVCLAGAFVTTD
                     PISGREPAAGLANSGGHRSPFTLLHGIADDVVPASASRRFATDLERHGWPVDLVELDA
                     DHGSIAGARYDRVADRYHAAEDPRTLDIAADVAARIAAVSG"
BASE COUNT          892 a         1698 c         1695 g          757 t
ORIGIN      
        1 gcttcgtgag gatgtcaccg gaacgctgca ggcggccgtc gcggaccccg atgtggccgg
       61 ccggctcggt caccttgcca aggccgaaca atggtccggc ttcggtgatt tcggttcagc
      121 gacagcggtc ttcacgacag cgcgcggcgg tacggccaga tcaacaacca agcaggcacg
      181 ccaagagcat ccgccgcagc cgcggcgggc ggacgaacat gcatcggcgc gccggacgga
      241 ggaggcacgc aaggcgttgg ccgcggccga gcgcgccaaa gacgacgccg ccaaccaatt
      301 atcaactcgc caaacggatc tggcggtcgc ccggttacgt cgtgacgaga tgcggcagcg
      361 cctcgaagag gctgagcgca acctcgacgc ggccgagaag gcttacagcg aggccaagca
      421 ggcgggccgg gacgccgctg atctggtcag ggaagcaaag gcacggctca aacagtccga
      481 gcgcacgcga taggtcagcg gacggcggcc cgcgcctcgg cgaggttgtc cgcgcccagc
      541 accagccggg tgttcgttgc gagatacacc agggtgaggt tcacccgggc ggtgccgagt
      601 tcgcaggcga cttcaccgag tgcgccgggc ctgtcctcga catccagaac cagcacctcc
      661 tgctcggcgg cgatcctgat gcccgcatcg tgcaacgcct cgaacgccgc cgtcgcatcg
      721 tcgatcagga tgtgcacctc ggcctgtcca ccgccgctgg ccacggcgca cagaccggcg
      781 atgttggcac ccgccttgcc aagcacgtcg ccgaggcggg caagctcccc gggttggtcg
      841 tccagataga gcgtcaggtc ggttgccatg accggctcct ttcgtcggac taccgacgat
      901 catcctccga cggcaacgac ataaccgact cctcacgaaa tcagggctca acgcacaccg
      961 cgggggtggc gacggcgagc gcgatcatca cagccgtcgg cgccgacgcg aatgcgtctt
     1021 cggcgccgcg agatccggac cggacatcgc ctgctcggtg cgcgggctcg ccggccggcc
     1081 catgcccgga ggtagccggt tcgccggcta tggtcgcgtt tctactgatc tgtaatactg
     1141 cgacgatgcg acgcgcattg ctgatcatcg ccgctgtgct ggcggtgttc gtcagtggat
     1201 gcggatccaa gatcaatccg gatggcgccg ccaaatcggt cgtcgatatc gtctcgcgtc
     1261 agacgaagtt caagccgaag gacgtcagct gcccatctgg cgtggacgcg gtggtcggag
     1321 ccgaattcga ctgcaaattc accggacccg agggcacgga gtacaccgcg cacctgaagg
     1381 tgacgaaggt cgagggcgac aacgtcgagt tctacgtcca atcaaagccc agctgatcca
     1441 atcgcttacc ggggcacctc gattgccgcg gacgcgagtg gcgcctaggc gacaatcggc
     1501 gggggctccg gcgcacccgg cgggggcggc ggcagttccg caccaggaag aggcggcggc
     1561 gggagctcgg cgccgggagg cggcggcggg agctcggcgc cgggaggcgg cggcagtaga
     1621 tcggcaccgg gaggaggagg cggcaacggc gctcccgccg gcggaggcag cggagcctcc
     1681 atccccacgg gaacgatgac gccctccggc gggagcggag cattcggcgg aggcggcatc
     1741 acgcccggag gcagctgctc ctctggcgca ggcgcatccg gcgccatctc gaggtacatg
     1801 tgcctggaga actgcgtctg gtaagcgcct ccgccaccga ccctcacgcc tccgccctca
     1861 ccggaggaga ccggcgttcc atctggcagg gtcgcggcgg tatggccacg gttccatccg
     1921 acgttgaggg cgccgggctt ggagccgtat tggaagccgc gggccttgag agcgccttcg
     1981 atattgccgg tgttgaaccg gtcgccgtag atcggacgac cggacgcggc attggtcacg
     2041 aaagaaacga gacccgagca gtctgtgccg gcagccgaat ctcctccgga gatgtagggg
     2101 gtgccggaca gctgctggac gacagtcaac agcgatgcgg tagcgacagc aaacatggcg
     2161 tcggacagta acagacatgc aattactgcc tcaaattctg tgatgccctt cactatatgg
     2221 ctgcgcagtg ggctgtttca ctctgtgcgc caaatcaaca atgctgccac acaaggttat
     2281 tgaatgtcag acgctaaggc gtgcgacatc attccgacca ctcgggatag cgtattccgt
     2341 tgtgcgccag tctgatttgg tcagatccat tgtcgatgag ccgattcggc gaccgtgcca
     2401 gtgctgacca agttcctccg gcgtcattct ggtaccggcc atgcagatct agcaaataaa
     2461 tcgctctgac caccggtgcg cagtcggccg gctgcatatt ggcaatatgt aacctctagg
     2521 ttgcgtaatg tgccaaaatt gcgggatgga cgaggtgttg cgggcgctgg ccgatccgag
     2581 ccggcgtcac cttctcgaca gcctcaacga gcgaaacggt cagacccttc gcgagctttg
     2641 cgcgagcctt tccttggcgc gccagtcggt gagcaagcac ctggcggtgc tcgaggcggc
     2701 ccggctggtg gccaccgagc ggcggggaag ggagaagttg cacttcctca acgccgagcc
     2761 gatcaaagcg atcgccggtc acctactcga aacctacgag cacgcgccct ctcgaccgcg
     2821 acaccgatgg gcgagctggc acgaggttct ggatcagccg taggccagta atagaggtat
     2881 gcgctgctcg gcactcgtga gcgagccgtg gtgaccgatc agtgacgact ccatcggctc
     2941 aacggtgcgc cggaccatcg tcgccgaccc gcgggcggcc gcgacgatgt cgccgatacg
     3001 catccggacg tcgtcgcgca ccgatccgcc gaaccagccc gccgcgatcg cctcgtcacg
     3061 cgatgccacc cacgcaccgt ccgcgagcgt ctcccgccac gccgcgagca ccgaatcggc
     3121 cgcaccgtgg tcgacataga cgtgccgcgc ccgcggctct ccaccgacgg cctgcacgcc
     3181 atcgagcagc gcggcccggc tgtcgatgtc cacggcgtca tcgtcgtcga ccgtcaccat
     3241 gccgtggtcg gcgactatcg cgagcagccc gccggacggg agaccctcca cgacggattc
     3301 caccagccga tcgacttccc gcaactgcat tcgccacgcg gccgaaccgg gaccgtaaag
     3361 atgcccgagc agatccacct cgccgtggta gccgtagcag aacccgccgt cggccacagc
     3421 cgcacggacg ccctcggcga ggtctccgag tgcccgcacc ccgacgtagc ggcctccgcg
     3481 cagaaccgcg cgggtcaggc ccgaaccggt gaactccgct ccggagacga cgctcaccgc
     3541 gacgcccgcc gaaaccgccc gttcgaaggt cgtcgccatc ggctgcacct gctcgggcgg
     3601 cacggtgtcc cgcagatcgt cgccccgggg gtgggggcgc cagcgcagag ggttgatcag
     3661 cccgacccct ggcagtcgga acgacagccc gaccagtccg tgctcgccgg aacggcagcc
     3721 ggtgccgatc gcggccagtc cggccacggt cgtcgacggg aaacccgcct gcagggtgcg
     3781 gccgcgcagc gccgccatca ccggggcgtc gtcggcgtag gcgtccagca ggtccgcgcc
     3841 gagtccgtcg atcaacagca cgcacgcacc gcgcacctcg gcggccaggt cgatccggcc
     3901 gtcgaattcg gccacaccca tcgccgccag cacggatggc accacgtcgg caaggtgagg
     3961 agtgtgcggg tccggctgcg gcaggtccac atcgcgagcc tgccatattc tttgcgctat
     4021 gccgcttcgg atcaggaccg tcgcgggcat cggactcacg ttgctgctcg gtgcctgcac
     4081 ctggtcgcag gccgcgcccg ccgcgcaact gacgaacggg cctgtgcccg acgacgggac
     4141 gttctcgatc ggccccgccg ccaccgactc gatcggcgga taccttcccg acggtcagac
     4201 gctgtcgccg ttcgatgcga ccaatccggt gatcggatgg ctcgacccgc cgttgctcgc
     4261 ggcggtgcag aacgcagcac gcagcgccaa ggacgacggg gtagacgtgc ggatcgcgtc
     4321 gggctggcgg acgaagggtt ttcagcagcg gctgttcgat gacggtgtgc gaacgtatgg
     4381 aagcgtcgag gccgcacagc agttcgtcgc ctcgcccgat acctcacagc acgtcctcgg
     4441 aaaggccgtc gacgtgagtc cggttgaagc cgatacctgg atgattcgca acggttctcg
     4501 gttcggcctg tgccagatct atgcgaacga gatatggcac ttcgagttgg cggccgacaa
     4561 ggatggagcc tgcccgccgt tgaggccgaa cgcggccggc tgattcgtct cagcccgaca
     4621 cggccgcgat ccgggccgcc acatccgctg cgatgtccag tgtcctggga tcctctgcgg
     4681 cgtggtagcg gtctgcaacc cggtcatagc gggcgcccgc gatagaaccg tggtcggcgt
     4741 cgagctcgac gaggtcgacg ggccagccat gtcgttcgag atcagtcgcg aaccgccggc
     4801 tggccgaggc aggcacgacg tcatcggcga ttccgtgcag cagcgtgaac ggcgaccgat
     4861 ggccgccgct gttcgccagg ccggcggccg gctcgcgacc ggagatgggg tcggttgtca
     4921 cgaacgcgcc ggccagacag acggtgtgtc gcaggcggac atcgtggtgg gacgcgtgaa
     4981 cggtcagccc cgcagcggcg agcccgccca tcgaccagcc cacgagggtg aggctgtcga
     5041 cc
//
LOCUS       DASYLX010000145         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2968627,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000145 DASYLX010000000
VERSION     DASYLX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968627"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGP27_03490"
     CDS             complement(<1..368)
                     /locus_tag="VGP27_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7850414.1"
                     /translation="MDSRTVVIVGSSVGGIRTAKALRSQGFTGPVLVIGAEAHLPYDK
                     PPLSKQFLAGAWDQSRITMLTAEDAEQADIELKLGSAAERLDMAGRAVVLADGTRVAY
                     DVLVVATGAAARPSPWSVDSG"
     gene            complement(368..565)
                     /locus_tag="VGP27_03495"
     CDS             complement(368..565)
                     /locus_tag="VGP27_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7850415.1"
                     /translation="MPRLHADYPLCQGYGNCVTGASDTFDVDDDGVVVLLRDTIPEQD
                     LIRVQDAVRSCPVNALSIETE"
     gene            complement(601..1407)
                     /gene="hcaB"
                     /locus_tag="VGP27_03500"
     CDS             complement(601..1407)
                     /gene="hcaB"
                     /locus_tag="VGP27_03500"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HEV7850416.1"
                     /translation="MTGWLTDRRALIVGGGSGIGRAVVEVFVAEGARVAVLERDQEKC
                     SALGAEQPDVPVTCGDASTRVANDDAVAAAVATFGGLDVLVNCVGVFDFYRSIADLDA
                     DVIDDAFDEIFRINVKSHVHSVKAALPALRQSPSPVILLTESTSGYYPGRGGVLYVAS
                     KFAVRGLVTALSHDLAPHIRVNGVAPGGTLGTDLRGLASLGTADRSIAGAPGRAEELA
                     ARVPLQVALSADDHAWSYVFLASHRARGITGDVIHPDGGMGVAGAPRKRS"
     gene            complement(1404..1634)
                     /locus_tag="VGP27_03505"
     CDS             complement(1404..1634)
                     /locus_tag="VGP27_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850417.1"
                     /translation="MTIGNEFAEVRITRVDTRNGSRLLIESLRSGQWVALCPLEAEAL
                     TWQGTATFSAMIGHPFGSLVGDEPAESAPESW"
     gene            complement(1709..2299)
                     /locus_tag="VGP27_03510"
     CDS             complement(1709..2299)
                     /locus_tag="VGP27_03510"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HEV7850418.1"
                     /translation="MSTVADTHSARSRLGTNASRVARTGRSLGFGDERHLLAHQWLVD
                     ETYLFDAQAYSDWLETLAEDIHYLMPVRVTTALAAGYDTAPGMAHFDEDKYSLSRRVA
                     RFLTEHAWTEDPPSRLRHHLSNVRTFATPDPDHLVVESATLLFRSRGDVREGAFLSAG
                     REDLLRCEEGGQWRLARRTIYVDDSVIRMQNLAVFL"
     gene            complement(2296..3699)
                     /locus_tag="VGP27_03515"
     CDS             complement(2296..3699)
                     /locus_tag="VGP27_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006867083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7850419.1"
                     /translation="MTDQSGILHDVRRGMIPAHIYNDREIFELEKRHIFGRAWIFVGH
                     ESEISLPGDYVVRRILEDSFIVTRDEGGAVRAHFNMCLHRGMQVCRAEMGNASHFRCP
                     YHGWSYRNDGRIVGLPFHQEAYGGEEGFARKGQRLLPAPNIAIYNGMIFLSLDPAAPP
                     LQDYLGDFRFYLDFYTKQSSGGIELHGPQRWRIKANWKIGAENFAGDMYHTPQTHTSV
                     VEIGLFREPNAQKRKEGALYWAGSGGGTTYKLPDGTLEERLRYVGYPDLMIERMKQSW
                     SSEQLAVIGTDGFMFSAASLYPNLSFVHNWPKVGDSDDVLPFITLRQWQPISEDETEV
                     LSWFAVDAAAPEEFKALSYKAYLMCFGSTGMFEQDDVENWVSLTNTAAGSMARRLLLN
                     SRMGLLADGTEVAPRLSADRFSGPGIARQGYSEFNQRELLRRWADDLARPIADPTLDA
                     PPNGTAANGEHPAVSRI"
     gene            complement(3995..4501)
                     /locus_tag="VGP27_03520"
     CDS             complement(3995..4501)
                     /locus_tag="VGP27_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV7850420.1"
                     /translation="MLSIDRLDVELLEMLARDARAGVVEMASMLRISRNTVQSRLKRL
                     EEGGLLTGYRPELDLAHAGVATQAFIGLEVQQGRLGPIVRALKGIPQVLEIHATTGRE
                     DLLVRAATETQAGLQQLIEQIVGTPGVVHSTTTLALTTPLPYRVVPLLKEITKDAGWG
                     RSTPASRG"
BASE COUNT          842 a         1512 c         1526 g          854 t
ORIGIN      
        1 cccgagtcca ccgaccatgg ggacggccga gcggccgcac cggtagcgac gacaaggacg
       61 tcatacgcca ctcgggtgcc gtcggcgagc acgaccgcgc ggccggccat gtccaggcgc
      121 tcggcggcgc tgccgagctt cagttcgatg tctgcctgct ccgcatcctc ggccgtcaac
      181 atcgtgattc tgctctgatc ccacgcgccc gcgaggaact gcttggacag cggcggcttg
      241 tcgtagggga ggtgcgcttc ggcgccgatc accaggaccg ggccggtgaa tccctgcgag
      301 cgcaacgctt ttgcggtgcg gatgcccccg accgacgacc cgacgatgac gactgtccgg
      361 ctgtccatca ctccgtctcg atgctcaagg cattcaccgg gcagctgcgc acggcgtctt
      421 ggacccgaat caggtcttgc tcaggaatgg tgtcgcgcaa caggacgacg actccgtcgt
      481 cgtcgacgtc gaacgtgtcc gatgcgccgg tcacgcaatt gccgtatccc tggcacaacg
      541 gatagtcagc gtggagtctg ggcacggaat cctctctcga tggtggacgg ggttggacgg
      601 tcaactgcgc ttgcgcggcg cgcctgcgac tcccatgcca ccatcggggt ggatgacgtc
      661 accggtgatg ccgcgggcgc gatgcgaggc caggaagacg tagctccagg cgtggtcgtc
      721 ggccgacagc gccacctgta gcggaacgcg ggccgcgagt tcttcggcgc ggccgggagc
      781 gcccgcgatg ctgcggtccg ctgtacccag gctggcgagc ccccgtaggt cggtgcccag
      841 cgtgccgccc ggggccacac cattgaccct gatgtgtggg gcgaggtcgt gggacagtgc
      901 ggtcaccagt ccgcggacgg cgaacttgga agcgacgtac agcactccgc cgcgacccgg
      961 gtagtacccg gacgtggact cggtcagcag gatgaccgga cttggggatt gccgtagtgc
     1021 ggggagcgcg gccttcaccg agtgcacatg actcttgacg ttgatgcgaa atatctcgtc
     1081 gaaggcgtcg tcgatgacgt cggcgtcgag atcggcgatg ctgcggtaga agtcgaagac
     1141 gcccacgcaa ttgacgagga cgtcgagacc accgaacgtc gccaccgcgg cggcgacggc
     1201 gtcgtcgttg gcgacacggg tactggcatc gccgcacgtg accggcacgt cgggttgctc
     1261 ggcgcccagg gccgagcact tctcctgatc acgctcgagg acggccacgc gggcgccctc
     1321 cgcgacgaag acctcgacga ccgctcggcc gattccggat ccgccgccga cgatcagagc
     1381 ccgccgatcg gtgagccagc cggtcaccac gactccggcg ccgactcagc gggctcatcg
     1441 ccgacaaggg atccgaacgg atgcccgatc atcgccgaga aggtcgccgt gccctgccac
     1501 gtcagcgcct cggcttccag cggacacaac gccacccact gacccgatcg aagcgattcg
     1561 atcagcaacc gcgaaccgtt gcgggtgtcc acccgggtga tgcgaacctc ggcgaattca
     1621 ttgccgatcg tgatcggttg tccgacagtg tgttcgagaa ggacgctgcg ctccgcgctc
     1681 gaccgcgcct gttcgtcgga cggcgtcgtc acagaaacac cgcgaggttc tgcatgcgga
     1741 tgacggagtc gtcgacgtag atcgtgcgac gggcgaggcg ccactgcccg ccctcctcgc
     1801 atcgcaacag atcctcacgc cccgcagaga ggaaggcgcc ctcgcgcacg tcacctctgc
     1861 tgcgaaacag cagggtggcc gactcgacga ccaagtgatc gggatcgggg gtggcaaagg
     1921 tgcggacgtt cgacaggtga tgacggagcc gcgacggcgg atcctcggtc caggcgtgct
     1981 cggtgaggaa acgggccacc cggcgagaca gtgagtactt gtcctcgtcg aaatgcgcca
     2041 tgcccggggc ggtgtcgtag ccggcggcca gtgccgtcgt gaccctgacc ggcatcaggt
     2101 agtggatgtc ctcggccagt gtctcgagcc agtctgagta ggcctgcgca tcgaacaggt
     2161 acgtctcatc caccagccac tggtgcgcca gaagatgccg ttcgtcgccg aagccgagcg
     2221 acctgccggt tcgtgcgact cgggatgcgt tggtgccgag ccgagaccga gcggaatggg
     2281 tgtccgcgac agtactcata tccgcgacac cgccggatgt tcaccgttgg ccgcagtacc
     2341 gttcggcggc gcgtcgagcg tcgggtcggc gatcggcctg gcaaggtcat cggcccagcg
     2401 ccgcaacagt tcgcgctgat tgaactcgct gtacccctgc cgcgcgatgc cgggcccact
     2461 gaatcggtcg gcggacagtc gtggcgccac ttcggtaccg tcggcaagca gacccatccg
     2521 gctgttgagc agtagccggc gagccatcga ccctgcagcg gtgttcgtca gcgacaccca
     2581 gttttcgacg tcgtcctgtt cgaacatgcc ggtgctgccg aaacacatca gataggcctt
     2641 gtaggaaagg gctttgaact cttcgggggc tgccgcgtca acggcgaacc aggacagcac
     2701 ctcggtctcg tcttcgctga tcggctgcca ctgccgcagc gtgatgaatg gcaatacgtc
     2761 gtcgctgtcg ccgaccttcg gccaattgtg cacgaaggac aggttcgggt atagcgatgc
     2821 cgcactgaac atgaagccgt cggtaccgat gacggcgagc tgttcggatg accaggattg
     2881 cttcatgcgc tcgatcatca ggtcggggta tccgacatag cgcaggcgct cttctaacgt
     2941 tccgtcggga agtttgtagg tggttccccc gccgctcccc gcccaataca gtgctccctc
     3001 tttacgtttc tgagcattgg gctcgcggaa caggccgatc tccacgaccg atgtgtgggt
     3061 ctggggcgtg tggtacatgt cgccggcgaa gttctcggcg ccgatcttcc agttcgcctt
     3121 gatccgccac cgttgcggcc cgtgcaactc gatgccgccg gaactctgct tcgtgtagaa
     3181 gtcgaggtag aaccggaagt cgccgagata gtcctgcagc ggaggcgctg cgggatccag
     3241 gctgagaaag atcatgccgt tgtagatcgc gatgttcggg gcaggcagga gccgctgccc
     3301 cttgcgtgcg aacccctcct cgccgccgta ggcttcctgg tggaacggca ggccgacgat
     3361 cctgccgtcg ttgcggtagg accagccgtg ataggggcaa cggaaatgcg atgcgttgcc
     3421 catctccgca cggcagacct gcattccgcg atgtaggcac atgttgaaat gggctcggac
     3481 agcaccacct tcgtcgcgag tgacgatgaa ggagtcctcc aggatccgac gcacaacgta
     3541 atcaccgggc agcgagatct cggattcgtg accgacgaaa atccacgccc gaccgaagat
     3601 gtgacgcttc tccagctcga agatctcccg atcgttgtag atatgagccg ggatcatccc
     3661 tcgtcgaacg tcgtggagga ttccagactg gtcggtcatg acgccttccc tgtacaccag
     3721 gtcgatgccg cttcgtggcc gaggcgcttc gggtaggaca gaccttctcg atgtcgtcga
     3781 cggagcgcgc cggaaaatac ctcgctcata gaagcgtcga cgtcgatgtg catgtgtatt
     3841 ccgaactctc cgctcgatcg tcgtgggaga ttgaacgcgt cgatgtagat gctaggacgt
     3901 aaagcatgga tagtacatat atcctattat atgttcaatg cttaacgccg gacaccacct
     3961 cgacccgccc accgggatcg ggcgggtcga cggcctaacc cctggaggca ggcgtcgacc
     4021 gtccccagcc ggcgtccttt gtgatctcct tgagcaacgg caccactcgg tagggcaacg
     4081 gtgtcgtcag cgccagcgtg gtcgtcgagt ggaccacccc aggggttccc acgatctgtt
     4141 cgatcaactg ctgcaggccg gcctgagtct cggttgcggc gcgcaccaga agatcctcgc
     4201 gcccggtcgt cgcatgtatc tccagcacct gcggtatccc tttgagtgct ctgacgattg
     4261 ggcccagacg cccctgttgc acctcgaggc cgatgaaggc ctgggtggcc actccggcgt
     4321 gcgcgaggtc gagctcgggt cggtagccgg tgagcagacc gccttcctcg agccgcttga
     4381 gacgggattg cacggtgttg cgcgagatac gcagcatcga cgccatttcc acgaccccgg
     4441 cccgagcgtc gcgcgcgagc atctccagca gctcgacgtc gagtcgatcg atgctgagca
     4501 tccgaaccac ctcactaagt gtcgtttggc atcagctgaa cggccgggtc agttgaattg
     4561 agtttagttg accaagaatc cgatcaaagc cagccttgac tgtacttccg aatcacaagc
     4621 cgtgtgccgg cttcatcaac acctggacgg tggatgacct tgtcagatct tcgtacggcc
     4681 ggaccggatc ccctcctgaa cgaacgctac cgggcttcct ccgggccggt tctg
//
LOCUS       DASYLX010000146         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3517457,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000146 DASYLX010000000
VERSION     DASYLX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517457"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VGP27_03525"
     CDS             <1..731
                     /locus_tag="VGP27_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850421.1"
                     /translation="GVPLEDRNKLFDWTNQMIGGDDPEFAEYDSLGATGELIWYAMQL
                     AQHKAEEPGEDIVSTLIDSEADGQLTEAEFGMFVVTLTVAGNETTRNSITQGMMAFTD
                     HPDQWELFKAQRPKTAADEIIRWASPITAFQRTALVDTELDGVQIKKGQRLVLFYRSA
                     NFDEDVFDDPYVFDVLRDPNPHLGFGGTGAHYCVGANLARMTIDLMFNAIADHIPELT
                     PLSAPERLRSSFINGIKHWQVEYR"
     gene            856..1140
                     /locus_tag="VGP27_03530"
     CDS             856..1140
                     /locus_tag="VGP27_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7850422.1"
                     /translation="MPTYRFSCVACGDFAVVRPMAESGASAACPECGRAAGRMFGSPA
                     LHAVDERLRRAYGASERSADAPQVVSSVPGQSRRATRITSDPRHARLPRP"
     gene            1155..2402
                     /gene="fmdA"
                     /locus_tag="VGP27_03535"
     CDS             1155..2402
                     /gene="fmdA"
                     /locus_tag="VGP27_03535"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEV7850423.1"
                     /translation="MPDVVFSVDQARSMRDQAVPGHNRWHPGVPPAANVRPGQHVRVE
                     CREWTDGQIGNNDSANDVRDVDLTHAHMLSGPLYVEGAEPGDLLVIDILDLGPVPQEV
                     GDAPGQGWGYTGIFAKANGGGFLTDQFPDAYKAIWDFAGQKCTSRHIPGVEFTGITHP
                     GLFGTAPSPDLLARWNARERALIDTDPERVPPLALPPLEENVLGGTATGDALAAIGRD
                     GARTVPARENGGNHDIKNFTRGSRVFYPVHVPGALFSGGDLHFSQGDGEITFCGAIEM
                     GGFIDFHIDLIKGGMETYGVTTNPILMPGNVEPRYTEFLTFIGIGVDHETDTQLYNDA
                     TTAYRNACLNAVAYLEKFGYSGAQAYLLLGAAPIEGRVSGVVDIPNACCSLYLPTAIF
                     DFDITPSASGPVRADRGSCAVSS"
     gene            2447..3373
                     /locus_tag="VGP27_03540"
     CDS             2447..3373
                     /locus_tag="VGP27_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850424.1"
                     /translation="MASSRAALITGANSGIGKEVARQFAESDVFDTIYLGCRNPAKAD
                     AALAELEQDTGKSIFTTVPLDTSDLGSVRAAVDAIQTPLQTVVMNAGGTGGSTPLALT
                     ADGVTEIFASNVLGHVVLLDQLIAANSLTGSAVLVGSEAARGVPKLGLPRPTFAEHSA
                     DEFASVIDGSFFNGRKSESRAYGQVKYLGALWMSELARRNTQLRLLTVSPGNTAGTDV
                     FRDLGTILTPLMNRVLMPHVLPRLGLAHPVGAGARRLVDATVGDDLDSGKFYASAANT
                     LTGPLIDQVAVMADFADADIQRHAYDAVHRFL"
     gene            complement(3383..>3826)
                     /locus_tag="VGP27_03545"
     CDS             complement(3383..>3826)
                     /locus_tag="VGP27_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV7850425.1"
                     /translation="VLGQRRYWRLKWTDLVIAEFPTPESLRLFVEVANHLIMGTGGTP
                     TIPMFIGQGANGLPEGTPGSKPGIGRGDGVMVAGDVRTLARQYCARGTVVQYEQYDLL
                     SHTSTVGLWLMQAIDWIHDRFAGQPAPNNCSSIPVGNPLDPLPLP"
BASE COUNT          691 a         1245 c         1221 g          669 t
ORIGIN      
        1 tcggagttcc gctggaggac cgcaacaagc tgttcgactg gaccaaccag atgatcggcg
       61 gcgacgaccc cgagttcgcg gaatacgact cactgggcgc aacaggcgag ttgatctggt
      121 acgccatgca attggcgcaa cacaaggccg aggagcccgg cgaggacatc gtcagcacat
      181 tgatcgactc cgaagccgac gggcagctga ccgaggccga gttcgggatg ttcgtcgtca
      241 cgctgacggt ggccggcaac gagacgaccc gcaactccat cacccagggc atgatggcct
      301 tcaccgacca tcccgaccag tgggaattgt tcaaagcgca gcggccgaag accgcggccg
      361 acgagatcat ccgctgggca tcccccatca ccgctttcca gcgcaccgcg ttggtcgaca
      421 ccgagctcga cggtgtgcag atcaagaagg gccagcggct ggtgctgttc taccggtcgg
      481 cgaacttcga cgaggacgtt ttcgacgacc cctacgtttt cgacgttctg cgagatccca
      541 acccgcacct ggggtttggc ggcaccggtg cgcactactg cgtcggcgcc aacctcgcca
      601 ggatgaccat cgacctgatg ttcaacgcaa tcgccgacca tattcccgag ctgacacccc
      661 tctccgcgcc agagcgcctg cggtcgagct tcatcaacgg gatcaaacac tggcaggtcg
      721 aataccggtg aacgcccgta ctgagggatt gtcgaggtga caactcggcc ccggtcacta
      781 ctatggccat agatgccgca gcgccgcagc ttcagcacat gtagccgtgc agcacgggtc
      841 tcgcggaggt gggcgatgcc gacttatcga ttttcctgtg ttgcgtgcgg cgacttcgcc
      901 gtcgtgcgtc cgatggctga atcgggggca tccgcagcgt gccccgagtg tgggcgagcc
      961 gcgggccgaa tgttcggttc acccgcattg cacgcagtcg acgaacggct gcggcgggcc
     1021 tatggggcga gcgaacgcag cgccgacgcg ccgcaggtcg tatcgtcggt gcccgggcag
     1081 tcgcgacgag ctacccgcat cacgtcggac ccacggcacg cccggctgcc tcgtccctga
     1141 atctggaggt tgtcatgccc gacgtcgtat tctcggttga tcaggcacgc tcgatgcgcg
     1201 atcaggctgt gccaggtcac aaccggtggc accccggtgt cccaccggca gccaatgtcc
     1261 gcccgggaca gcatgttcgg gtggaatgcc gggaatggac ggacggtcag atcggcaaca
     1321 acgactccgc gaacgatgtt cgcgacgtcg acctgacaca cgcccacatg ctgtcaggcc
     1381 cgctttatgt ggaaggcgcc gagcccggcg accttctcgt catcgacatc ctcgacctcg
     1441 gccccgtgcc gcaggaggtt ggcgacgctc caggtcaggg ctggggctac accgggatct
     1501 tcgccaaggc caacggcggc ggctttctca ccgaccagtt tcctgacgcc tataaggcga
     1561 tctgggactt tgccggacag aaatgcacgt cgcgccacat tcctggcgtc gagttcaccg
     1621 gcatcacgca tccggggctg ttcggcacgg cgccgtcacc cgacctgctg gcacgctgga
     1681 acgcccgcga acgcgcgctg atcgatacgg accccgagcg cgtgccgccg cttgcgttac
     1741 cgccgctgga ggaaaacgtg ttgggtggca ccgcaaccgg agatgcgctg gctgccatcg
     1801 gtcgcgacgg cgcgcggacc gtcccggctc gggagaacgg cggcaatcac gacatcaaga
     1861 acttcacccg tgggtcgcgg gtgttctacc ccgttcatgt ccccggcgcc ctgttctcag
     1921 gcggcgatct gcacttcagc cagggcgatg gcgagatcac cttctgcggc gcgatcgaga
     1981 tgggtggatt catcgatttc cacatcgatc tgatcaaggg cggcatggag acatacgggg
     2041 ttaccacgaa cccgatcctg atgccgggca acgtggagcc gcgctacacg gagtttctga
     2101 cattcatcgg tatcggcgtc gaccacgaaa ccgacaccca gttgtacaac gacgcgacga
     2161 cggcgtaccg caatgcctgc ctcaacgccg tcgcctatct ggagaagttc ggctactccg
     2221 gtgcgcaggc ctatctgctc ctcggcgcgg cgccgatcga gggacgggtc agcggcgtcg
     2281 tggacatccc caacgcgtgc tgttccctct atttgcccac agctatcttc gatttcgaca
     2341 tcacgccgtc ggccagcggc ccggtacggg cagatcgcgg cagttgcgcc gtgtcgtcgt
     2401 gacattcttc cgggcatacc gtcggcccgt cctgacatag tggggcatgg cttcatcaag
     2461 ggcggcgctg ataaccggtg cgaactccgg tatcggtaaa gaggtcgctc gccaattcgc
     2521 cgagtccgac gtcttcgaca ccatctacct tggctgtcgc aatccggcca aggctgacgc
     2581 ggctctcgcc gaactcgaac aggacaccgg caagtcgatc ttcacgaccg tgccgctgga
     2641 cacaagtgac ctcggctcgg tgcgtgcggc agtcgacgcg attcagacac cgctgcagac
     2701 cgtcgtcatg aacgccgggg gaaccggagg ttcgacgccc ttggctctga ccgccgacgg
     2761 agttaccgag atctttgcgt ccaacgttct cggccacgtg gtgctcctcg accaactgat
     2821 cgcggcgaac tcgctgacgg ggtcagccgt tctggtcggc agtgaggccg cgcgcggtgt
     2881 gccgaaactt gggctccccc gacccacgtt cgccgagcat tcggcggacg aattcgccag
     2941 cgtcatcgac gggtcgttct tcaacgggcg gaaatccgaa tctcgcgcat acggacaggt
     3001 caaatatcta ggcgccctgt ggatgtcgga gcttgcgcgt cgcaatacgc agctgcgcct
     3061 tctcacggtg agcccgggga acaccgcggg caccgacgtt ttcagagatc tggggacaat
     3121 tctgacaccg ctgatgaatc gcgtcctgat gccgcacgta ctgccgaggc tcggcctcgc
     3181 tcatcccgtt ggcgcaggtg cgcggcggct ggtggatgcc acggtcggag acgacctcga
     3241 cagcggaaag ttctacgcga gcgccgccaa cacattgacc ggacccctga tcgatcaagt
     3301 ggccgtcatg gccgatttcg ccgatgcgga tatccaaagg catgcctacg acgcggtcca
     3361 ccggttcctg tagttgcgca ccctagggaa ggggaagcgg gtcgagggga ttgccgacgg
     3421 ggatcgacga gcagttgttc ggcgcgggct gaccggcgaa ccggtcgtgg atccagtcga
     3481 tggcctgcat cagccagagt ccgacggtcg aggtgtggct gagaaggtcg tactgctcgt
     3541 actgcacgac ggttccgcgc gcgcagtact ggcgcgcgag cgtgcggacg tcgccggcga
     3601 ccatcacgcc gtcgccgcgg ccgatgcccg gcttgctgcc gggagtgccc tcgggaagac
     3661 cgttggcgcc ctgcccgatg aacatcggaa tggtaggcgt gccgcccgtg cccatgatga
     3721 ggtggttggc cacctcgacg aacagccgaa ggctctccgg cgtcgggaac tccgcgatga
     3781 cgagatcggt ccatttcagc cgccaatacc gacgctggcc gaggac
//
LOCUS       DASYLX010000147         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2311241,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000147 DASYLX010000000
VERSION     DASYLX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311241"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGP27_03550"
     CDS             <1..463
                     /locus_tag="VGP27_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEV7850426.1"
                     /translation="RNNDFNYKQMDPIGYAVPLGSHIRRMNPRDTAVNMNRRRMIRRG
                     GTYGPALPQGSPEDGIERGVAAFVGCGSLVRQFEFAQNVWINDPKFHELENEHDPIIG
                     SQDGTLEFTIPKRPIKRKITGLPAFTTVRAGAYFFLPGMRALRWLSTLGDA"
     gene            564..1721
                     /locus_tag="VGP27_03555"
     CDS             564..1721
                     /locus_tag="VGP27_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEV7850427.1"
                     /translation="MSDHGSGPRASADPVLDISDFYAFPSPELPGRLVLAMNVFPFAM
                     PNALFSDAVDYRFRLRTVATGANGSQPAFIVDDAEGVVSFTFSAPQAVNGALTQAGLC
                     RLASGETISVRVGDDPGAEQNGVRVFAGCRLDPFFLDQGISGGIRNTRRLPTEIPGRN
                     SLDGLNVLTIVIEGPVSRLFGVDDGSLFAVVSETTTLGSPMVRIERVGRPEIKNFIML
                     DKQSDTVNRDLEIRDIYNAEDTFKLKPDYIGAYCARLNSNLAFYDGLDGKIDWPLDAE
                     GNHPLTSLLLADFLVVDMSKSFDDEGYLEIEQAVLRGVAHETCGGRTMNVDIADRFLT
                     WMVNNGNGPAITDNVDQATVPASQSFPYAQPGNPNPPAVPNLALPAEARGS"
     gene            1838..2560
                     /locus_tag="VGP27_03560"
     CDS             1838..2560
                     /locus_tag="VGP27_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850428.1"
                     /translation="MPAIRTTDGLIAVQNLHAQIDGLERQTQGHALSPGRRAHLVELL
                     LLRGHLLGRIADYEHADALAEQLVRDTPTEAIAYVARARTGGTFHRFAESLADLDRAE
                     QLGYDPILLLPDGASALHETGRSGDAFALLAAIPDRRHDVTTLGALAVLYAAYGETVR
                     ADETFTTARARYRGVSPFSIAMLDFQRGRMWLEHGEPGLARCWLAAARRRLPSYVPAQ
                     EHLAAAEAALGASDVPIARRSA"
     gene            2620..3048
                     /locus_tag="VGP27_03565"
     CDS             2620..3048
                     /locus_tag="VGP27_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850429.1"
                     /translation="MRELLAAAIAILLPLAILLSMFDLGLGAGRPHALRLLVRPARLA
                     RFVLVTFVVMPALTVLLVLLASLPQPVIEGLVLMSSCPPGLGPSQKALKLTGDSEVGL
                     AWQALAIPLSVVTIPLSLFVIQSAFGGASFRAPQRCCGRS"
     gene            3030..3419
                     /locus_tag="VGP27_03570"
     CDS             3030..3419
                     /locus_tag="VGP27_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850430.1"
                     /translation="MLWKVVTIFLLPVVLGIAVGMLWPGSTPALLKRLRPIATIGQLT
                     LLVLALLASGPAIVGLGLPAIGMVVVVVGSSIVVGHLLGGPTESSRPILASTLAFALA
                     GAGTRAGRRQPVGDGGHARGSDDRPRR"
     gene            3474..3638
                     /locus_tag="VGP27_03575"
                     /pseudo
     CDS             3474..3638
                     /locus_tag="VGP27_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          600 a         1273 c         1218 g          667 t
ORIGIN      
        1 gcgcaacaac gacttcaact acaagcagat ggaccccatc gggtacgccg taccgctcgg
       61 ttcccacatc cgccgtatga acccgcgtga cacggccgtc aacatgaatc ggcggaggat
      121 gattcgtcga ggcgggacgt atggaccggc cttgccgcag ggctctcctg aggacggcat
      181 cgagcgtgga gtcgccgctt ttgtggggtg tggaagtctg gtccgccagt tcgagttcgc
      241 tcagaacgtc tggatcaacg acccgaagtt ccacgagctc gaaaacgagc acgacccgat
      301 catcgggagc caggatggca ccctcgagtt cacgatcccg aagcggccga ttaaacgcaa
      361 gatcaccggg ctgccagcgt tcacaaccgt acgtgcaggc gcctacttct tcttgcccgg
      421 catgcgggcg cttcgttggc tgtcgacact tggcgatgcg tgaggccgcc ggtggccaaa
      481 cgacgcgaac tggcttttgc ctcaccctcg ctgcacgttg atgacgcctc ggccactagt
      541 gtcaaactca ggaggccatt cgcatgtctg accacggttc tggaccccgg gcgtcagctg
      601 acccggtgtt ggacatttcg gacttctacg cgttcccgag cccagagctg cctggacgcc
      661 tggtgctcgc aatgaacgtc ttccccttcg ccatgccgaa cgcgctgttc tcagacgctg
      721 tcgactaccg cttccgactg cggacggttg cgaccggtgc gaacggcagt caaccggcct
      781 tcatcgtgga cgacgcggag ggggtcgtca gcttcacgtt cagcgcaccg caagctgtca
      841 acggcgcgtt gacccaggct ggcctgtgtc ggctggcatc cggcgaaacg atctccgtcc
      901 gcgttggcga cgatcccggc gccgagcaga acggcgtacg agtgttcgct gggtgccgcc
      961 tggacccctt cttcctcgac cagggcatct ctggggggat ccggaacacc cgaaggctgc
     1021 cgaccgagat cccaggccgc aactcgctcg acggcctgaa cgtgctgacc atcgtcatag
     1081 aagggccggt ttcccgcttg ttcggcgtcg acgatggttc gctgttcgcc gtcgtgtcgg
     1141 aaaccacgac cctcggctcg ccgatggtgc ggatcgagcg agtcgggcgc cccgagatca
     1201 agaacttcat catgctcgac aagcagtccg acactgtcaa ccgcgacctt gagatccgcg
     1261 acatctataa cgctgaggac acgttcaagc tgaagccgga ctacatcggg gcgtactgcg
     1321 cgcgcctgaa cagcaatctg gcgttctacg acgggctgga cggaaagatc gactggccgc
     1381 tcgacgccga gggcaaccac ccgctcacca gcctgttgct ggcagacttc ctggtagtgg
     1441 atatgtcgaa gtcgttcgac gatgaagggt acctggagat cgagcaagcc gtgctgcgcg
     1501 gcgtcgcaca cgagacctgc ggtggccgga cgatgaacgt cgacatcgcc gaccggttcc
     1561 tcacctggat ggtcaacaac ggcaacgggc cagcgatcac cgacaacgtc gatcaggcca
     1621 cggtgccggc ctcacagagc tttccctacg ctcagccggg caacccgaac ccgccggcgg
     1681 tgccaaacct tgccttgccg gccgaggcaa gggggtcctg actgagcgcc gagtggcctc
     1741 ggctccctct gcgccggact catcccggcg cgtcgcgcat gaaagatttc cgatgccgtt
     1801 accgagcacc gtcgaccaac agattcgcca acgcgtcgtg ccggccattc gaacgacgga
     1861 cggcttgatt gctgtgcaga atcttcatgc acagatcgac ggcctggagc gacaaacgca
     1921 aggccacgca ttgtcgcccg gccgtcgtgc ccatctcgtc gagctgctcc tactccgggg
     1981 ccacctgctc gggcggatcg ctgactacga gcatgccgac gcgctggcag aacagctagt
     2041 ccgtgatacg ccgaccgagg caatcgcgta cgtcgcacgg gcaaggactg gcggcacgtt
     2101 ccaccgcttc gccgagagcc tcgctgacct cgaccgcgcc gagcaactcg gctatgaccc
     2161 gatcctgcta ctccccgacg gtgcatcagc cctccatgag acaggccgct cgggcgatgc
     2221 gtttgcactg ctcgcggcca tccctgacag acgacatgac gtcaccacgc tcggggcgct
     2281 tgctgtgctg tacgcggcgt acggagagac agtcagagcc gacgagacgt tcaccaccgc
     2341 gcgggctcgt taccgtggcg tctcgccgtt ctccatcgcg atgctggact tccagcgcgg
     2401 tcggatgtgg ctcgaacacg gcgaacccgg gctcgcgcgc tgctggctcg cggcggcgcg
     2461 ccggcggctg ccgtcgtacg tgccggccca ggagcacctc gcggcggcgg aggccgcgct
     2521 cggcgcgtcc gacgtcccga tcgcccggcg cagcgcttga cacacgcctc tgacggtcat
     2581 gttgacgctg cccagcgcgt aggtgcaccg ggagcagatg tgcgtgagct gctagccgcc
     2641 gcgattgcca ttttgctacc gctggcgatt ctgctcagca tgttcgacct cgggctgggc
     2701 gcgggtcgtc ctcacgccct gcgcctgctg gtgcgtcctg cccggctggc gcgcttcgtc
     2761 ctggtgacgt tcgtcgtcat gccggcactc acggtgctcc tcgtcctgct ggcgtcgttg
     2821 ccacagccgg tcatcgaggg actggtgctg atgtcgagct gtccgcctgg tctgggcccg
     2881 tcacagaagg cgctcaagct caccggcgac tcggaggtcg gcctcgcctg gcaggcgttg
     2941 gccatcccgc tctcggtggt gacaattccg ctctccctct tcgtcattca gagcgcgttc
     3001 gggggagcat cgttccgagc gcctcagcgg tgctgtggaa ggtcgtgacg atcttcctgc
     3061 tcccggtggt gctcggcatc gccgtgggca tgctctggcc gggcagtaca ccggcgctgc
     3121 tcaagcgctt gaggccgatc gccacgatcg gccagctcac cctgctggta ctggccctgc
     3181 tcgccagcgg gccagcgatc gtcggcctgg gactgcccgc gatcggcatg gtggttgtgg
     3241 tcgtcgggtc atcgatcgtc gtcggccacc tgctgggcgg cccgaccgag tcgtcgcggc
     3301 cgatcctcgc ctcgacgctc gcctttgcgc tggccggcgc cggcactcgc gctggccgcc
     3361 gtcaaccagt cggcgatgga ggtcacgccc gtggtagtga cgatcgtcct cgccggtgac
     3421 gtgctgatgg tgccggacga gcgctggctg gcgaggtcgg cgcctgccca agcacgccgt
     3481 tgcagctgtg gttctgggcg gcgtacctga tggccagcgg aactccgggg atctccgcgg
     3541 tgcagctgca gcggcaactg gaccgcggcc gctacgagac cgcctggatg ctcctgcaga
     3601 agctgcgccg cgcgatggtc gatccggagc ggcgccgctg accggtgggg tcttcgtcac
     3661 cgaggtcgtc gcggccaggg cggtggtgca cacagacggc tggaacggcg accggcggct
     3721 gcgcacgcgg ggctacgacc accgccctca cagccaac
//
LOCUS       DASYLX010000148         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1652899,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000148 DASYLX010000000
VERSION     DASYLX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652899"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGP27_03580"
     CDS             complement(<1..283)
                     /locus_tag="VGP27_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7850431.1"
                     /translation="MSQEVGSANGAVTVRRDGALLRLTLDRPAGRNSLSHTMVDALVD
                     ALAAAATDDALRVIHIRGEGDDFCAGADWVATNSGGARPRTGDLVRRIPH"
     gene            373..1023
                     /locus_tag="VGP27_03585"
     CDS             373..1023
                     /locus_tag="VGP27_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850432.1"
                     /translation="MADGDTPRFGEQPLAQTVAAAGALRRLAGLLLSLERPHPAVDAM
                     LAQFTEWENELSAAIPPDATPRIGDGGTDTQRVYLAHAFDIGAYNPAFPEYEFDRITA
                     ETAEGRVTFPVVYEGPPGLVHGGFLGVFFDCVIQHQNCATELSGKTRSLNVKFRRPTP
                     ILTELRFDIARAQGDHGVRSTARLLLDDEVLCVGEAATLALSPDRLTGFNFGKRRV"
     gene            1028..1783
                     /locus_tag="VGP27_03590"
     CDS             1028..1783
                     /locus_tag="VGP27_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEV7850433.1"
                     /translation="MELQGIRQPRVAEIVASRLRDEILSGRLKELPSQESLLQQFGVS
                     PPALREAIHILESDGLISVRRGNVGGAIVHQPSADRTAHMISMVLQTRGSTPADVSEA
                     LLHLEPICAGMCASREDRMTAVVPYLQAGIDSQAEQFDDLSAYVPNARRFHEALVSRC
                     GNDPMILLIGSLELVWSAHESSVWSDDDVNHKTMRAALRDHQRLLDAIRDGNAPRAVR
                     LAQDHLSRARRGTLAAGADKTIQAKLISRSAGQ"
     gene            1780..>2223
                     /locus_tag="VGP27_03595"
     CDS             1780..>2223
                     /locus_tag="VGP27_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7850434.1"
                     /translation="MTASTDDRVLFDVDPDKRIATITLNNSRQRNSYDAAMRDAVARC
                     LDRVADDDDITVVLLRGADGVFSTGADMNNAYGWYGDERPREEKKSRPSQRRRLTVDR
                     KSFGFYHNLMGFPKVTVGEISGYALGGGFEMALMTDISVIARDTKI"
BASE COUNT          407 a          736 c          713 g          367 t
ORIGIN      
        1 tatgggggat gcgccggacc agatcgcctg tgcgcggccg tgcgccgccg gaattggtgg
       61 ccacccagtc ggcgcccgcg cagaagtcgt cgccttcacc gcgaatgtga atgacccgca
      121 acgcatcgtc ggtggcggcc gcggccagcg cgtcgacgag cgcatcgacc atcgtgtgac
      181 tcagcgagtt gcgtccggcc gggcggtcga gcgtgagccg caggagcgcc ccgtcccggc
      241 gcacggtcac cgcgccgtta gcggagccga cctcttgact cactgccggg tccctttctc
      301 gagccgtata cggataccca tacagtaggc taaacgatta ctatcctgta caagatagaa
      361 gggaaagccc ggatggcgga cggtgatacc ccccggttcg gcgagcagcc gcttgcccag
      421 accgtggccg ccgcaggggc gctccgtcgt ctcgcaggtc tcctgctgtc gctcgagcgg
      481 ccccaccccg cggtcgacgc catgctcgcg cagttcaccg agtgggagaa cgagctgtcg
      541 gccgcaattc cgcccgatgc cacacctcgg atcggtgacg gcggcaccga cacccaacgc
      601 gtctaccttg cacacgcatt cgacatcggc gcctacaacc ccgcctttcc cgagtacgaa
      661 ttcgaccgca tcaccgccga gacggcggaa ggccgcgtca cctttcccgt cgtctatgaa
      721 gggccaccgg gtttggtgca cggcggtttc ctcggggtct tcttcgactg cgtgatccag
      781 caccagaact gcgccaccga gctctcgggc aagacgcggt cgctgaacgt gaaattccgg
      841 aggccgacgc ccatcctgac cgagctgcgc ttcgacattg ctcgcgcaca gggcgatcat
      901 ggggtgagat cgacggcgcg gctgcttctg gacgacgagg tgctgtgcgt cggcgaagcc
      961 gcgacgctgg ccctgtcgcc ggacaggctc accgggttca acttcggcaa gcgaagggtc
     1021 tgaccccgtg gagttgcagg gcattcgcca gccgcgcgtc gcagagatcg tggcgtcgcg
     1081 gctacgcgac gagatcctct ccggccgcct gaaggagttg ccgtcgcaag aaagtctcct
     1141 ccagcagttc ggggtgagtc cgcccgcgct gcgcgaggcc atccacatac tcgaaagcga
     1201 cggcctgata tcggtgcgcc gcggcaatgt cgggggagcg atcgtccatc agccgtcggc
     1261 cgaccgcaca gcacacatga tcagcatggt gctacagacg cgagggtcca cacctgcgga
     1321 tgtcagcgag gcgctactgc acctcgagcc gatctgcgcg ggaatgtgcg cctcgcgcga
     1381 agatcgaatg accgccgtcg taccgtatct gcaggccgga atcgactctc aagcagaaca
     1441 attcgacgac ttgtcggcct acgtgccgaa tgccaggcgg tttcacgaag ctctggtgtc
     1501 gcgctgcggc aacgatccga tgattctgct gatcggctcg ctggaactgg tctggtcggc
     1561 ccacgagtcc tcggtgtgga gcgacgacga cgtgaaccac aaaaccatgc gggccgcact
     1621 gcgcgatcac caacggctat tggacgccat ccgcgacggc aacgcaccgc gcgcggtacg
     1681 gctggcgcag gaccatcttt cgagggcccg gcggggcacg ctcgccgccg gggccgacaa
     1741 aaccattcag gcgaaactga tttcgaggag tgcgggacaa tgacggcttc taccgatgat
     1801 cgcgtgctct tcgacgtcga tccggacaag cggatcgcga cgatcaccct gaacaattcc
     1861 aggcagcgca actcctacga cgccgccatg cgcgacgccg tcgcccggtg cctcgatcgt
     1921 gtcgccgatg acgacgacat cacggtggtg ctgttgcgcg gagcggacgg ggtgttcagc
     1981 accggcgcgg acatgaacaa tgcctacggt tggtatggcg atgagcgccc ccgcgaagag
     2041 aagaagagtc gcccgagcca acgccggcgg ctgactgtcg atcgaaagtc cttcggcttc
     2101 taccacaacc tgatggggtt tccgaaggtc accgtcggcg aaatcagcgg ctacgcactc
     2161 ggcggcggtt tcgagatggc tttgatgacc gatatctccg tgatcgcccg cgacaccaag
     2221 atc
//
LOCUS       DASYLX010000149         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1652927,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000149 DASYLX010000000
VERSION     DASYLX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652927"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..805
                     /locus_tag="VGP27_03600"
     CDS             44..805
                     /locus_tag="VGP27_03600"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7850435.1"
                     /translation="MGYADELFDLTDRVVLVTGGSRGLGREMAFAAAQCGADVIIASR
                     KLEACQSTAEEIASATGRKAMPYALHVGRWDQLDGLVDAAYERFGKVDVLINNAGMSP
                     LYESLDSVSEKLFDTVINLNLKGPFRLSTLVGARMVADGGGSIINVSSSGSRRPRPEQ
                     LPYSAAKAGLNALTEGLALAFGPTVRVNTLMPGPFLTDISKAWDIPATTKGAQNFALK
                     RLGNPAEIVGAALYLASDASSYTSGSIIRVDGGIP"
     gene            complement(809..2686)
                     /locus_tag="VGP27_03605"
     CDS             complement(809..2686)
                     /locus_tag="VGP27_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEV7850436.1"
                     /translation="MIQKPPTAVIEKANRGHVDNLPFCDTSDFRDADRGFIGALQPCV
                     IKAADGRVVWDNDAYAFLSGEAPTTVHPSLWRQSQLCAKQGLYEVVEGIYQVRGLDLS
                     NISFIEGDTGIVVIDPLVSTETAAAALALYRTHRGDRAVSAVIYTHSHVDHFGGVLGV
                     TTQADVDAGEVVVIAPEHFTQHAVQENVYAGTAMGRRAGYMYGGALARGPQGQVGCGL
                     GQVASAGEVALIVPTLHIRETGETHTIDGIEIEFQMAPGTEAPAEMHFYFPKFRALCM
                     AENATHNLHNLLTLRGALVRDPHGWAGYLTEAIDAFSDRADVVFASHHWPTWGKDGIV
                     EFLSLQRDLYSYLHDQTLRQLNQGFTGIEIAEDFQMPPALEKAWHAHGYYGSVSHNVK
                     AVYQRYMGWFDGNPGRLWAHPPEAIGPRYVEAMGGIDNVVETARTAFDTGDFRWAATL
                     LDHAIFTDHHHATARELYADTLEQLAYGAENAVWRNFFLSGATELRDGNFGTPTQPAS
                     PSMLAQLSPEQMFDTFAINIDGPRAWNLDVAVDVTFLDTATNYRLTLRNGVLVHRKAP
                     ADQSTAQSTVRLANKMRLLAFAVGDTDSPGIEISGDPEALPSIIAVVDRPDPGFNIIE
                     P"
     gene            complement(2713..3420)
                     /locus_tag="VGP27_03610"
     CDS             complement(2713..3420)
                     /locus_tag="VGP27_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7850437.1"
                     /translation="MSGRPVRRFVDGLLRGQRTGRARPDDLDAQEMKTAIEMRAARTG
                     SDLPREEFITDLHRRLAEQMGETQQAENRSPWGPSGTRRQVVIGTGLAAASAATGIVV
                     GRNLLAPTRPPTENATPSQDVLQPNAGSWHAVGASADLPKGGALAFDLGAVNGFVHRV
                     DAEVEAVSGICTHQGCKLWLDAPDGRLRCPCHSTSFSLDGETLTHQLPTPPPPLPKFR
                     VREINGVIEVFAPTEPA"
BASE COUNT          567 a         1133 c         1159 g          620 t
ORIGIN      
        1 tgcaccgtga caccatatga ccaccgaagc gaggagcacc gttatgggtt acgccgacga
       61 actgttcgac ctcaccgacc gggtcgtcct ggtcaccggc ggcagtcgcg gactcggtcg
      121 cgagatggcg ttcgccgcgg cgcaatgcgg cgccgacgtc atcatcgcca gtcgcaagct
      181 ggaggcctgt cagtccaccg ccgaggagat cgcctcggcc accgggcgca aagcgatgcc
      241 gtatgcgctg cacgtcggcc ggtgggatca gctcgacggg ctggtcgacg cggcttacga
      301 acggttcggc aaggtggacg tcctgatcaa caacgcgggt atgtcgccgc tgtacgaatc
      361 gctggactcc gtctccgaga agctgttcga caccgtcatc aacctcaacc tgaagggtcc
      421 gttccggttg tcgaccctgg tgggtgcgcg catggtggcc gacggtggcg gatcgatcat
      481 caacgtgagc tcgagcgggt cgcggcgtcc acggcccgag cagctgccgt actcggcggc
      541 caaggcggga ctcaacgcgt tgaccgaggg gctcgcactc gccttcggcc cgacggtgcg
      601 ggtcaacacg ttgatgccag ggccctttct caccgacatc agcaaggcat gggacatccc
      661 ggcgacgacc aagggcgcgc agaacttcgc gctcaagcgg ctcggcaatc cagccgagat
      721 cgtcggggcc gctttgtatc tggcatcgga tgcgtcgagc tacaccagcg ggtcgatcat
      781 ccgggttgac ggtgggatcc cttagccgtc acggctcgat gatgttgaag ccgggatccg
      841 gccggtccac cactgcgatg atcgagggca aagcctctgg gtcgccggag atttcgatcc
      901 ccggcgagtc ggtgtcgccg acggcgaacg cgagcagccg catcttgttc gcgagcctga
      961 cggtcgactg cgctgtcgac tggtccgctg gagccttccg gtggacgagc acgccgttgc
     1021 gcagcgtcag ccggtagttg gtcgcggtgt cgaggaacgt gacgtcgacg gcgacgtcca
     1081 gattccaagc ccgcggaccg tcgatgttga tcgcgaacgt gtcgaacatc tgctcgggcg
     1141 acagctgggc gagcattgac ggagacgccg gctgggtcgg tgtgccgaag ttgccatccc
     1201 gcagttccgt tgccccggag aggaagaagt tgcgccacac ggcgttttca gcaccgtacg
     1261 ccagctgttc gagcgtgtcg gcgtagagct cacgggcagt ggcgtgatgg tggtcggtga
     1321 agatcgcatg gtcgagcaac gttgccgccc agcggaagtc accggtgtca aacgccgttc
     1381 gcgctgtctc gacgacgttg tcgattccgc ccatcgcctc gacatagcgc ggtccgatgg
     1441 cctccggcgg gtgcgcccac aaccggcccg ggttgccgtc gaaccacccc atgtagcgct
     1501 ggtacacggc cttgacgttg tggctgaccg atccgtagta accgtgtgca tgccatgcct
     1561 tctccagtgc gggaggcatc tggaagtcct cggcgatctc gattccggtg aatccctggt
     1621 tgagctgccg cagtgtctgg tcgtgcagat aggagtacag atcccgttgc agcgacagga
     1681 attcgacgat cccatccttg ccccacgtcg gccagtgatg ggatgcgaaa accacgtcgg
     1741 cccgatcgct gaacgcgtcg atggcctcgg tcagataacc ggcccagccg tgcgggtcgc
     1801 gcaccagtgc accacgcaac gtcaacaggt tgtgcaggtt gtgggttgcg ttctccgcca
     1861 tgcacagtgc gcggaacttc gggaagtaga agtgcatctc ggcgggcgcc tcggttcccg
     1921 gcgccatctg gaactcgatc tcgatcccgt cgatcgtgtg tgtctcgccg gtttcgcgaa
     1981 tgtggagcgt gggcacgatc aacgcaacct cgcctgcgga ggcaacctga cccagtccgc
     2041 agccaacctg cccctgcggc ccgcgtgcca acgcgccccc gtacatgtat ccggcgcggc
     2101 gccccattgc cgttccggcg tacacgttct cctggacggc atgctgcgtg aagtgctcgg
     2161 gcgcaatcac cacgacctcg cccgcatcga cgtcggcctg cgtggtgaca ccgagaacgc
     2221 caccgaagtg gtcgacgtgg ctgtgggtgt agatgacagc gctgaccgcc cgatcccctc
     2281 gatgcgttcg gtacagcgca agggccgccg ccgccgtctc cgtcgacacg agcggatcga
     2341 tgacgacgat gccggtgtcg ccttcgatga aactgatgtt ggagaggtca aggccgcgga
     2401 cctggtagat gccctcaacc acttcgtaga gtccttgctt ggcgcacagt tggctctgcc
     2461 gccagaggct ggggtgcacc gttgtcggtg cctcaccgct caggaacgca taggcatcgt
     2521 tgtcccatac caccctgccg tcggccgcct tgatgacaca cggctgcagt gcgccgatga
     2581 atccgcggtc ggcgtcgcgg aaatccgagg tgtcgcagaa cggcaagttg tcgacgtgcc
     2641 cgcggttcgc cttttcgatg acggcggtgg gcggcttctg aatcatgccg ccgacattgc
     2701 catcaaatcc agctatgcgg gttcggttgg cgcgaaaacc tcgatcacac cgttgatttc
     2761 acgcacgcgg aacttcggca gcggcggtgg tggtgtcggc agctgatgag tcagcgtctc
     2821 gccgtccagc gagaaggacg tcgagtggca cgggcaccgc agccggccgt cgggtgcgtc
     2881 cagccacagc ttgcagccct gatgggtgca gatgcccgag acggcctcga cttcggcgtc
     2941 gacgcgatgg acgaacccgt tgaccgcgcc gaggtcgaac gcgagcgctc cacccttcgg
     3001 caggtcggca ctcgcgccga cggcatgcca cgacccggcg ttcggttgga gcacatcctg
     3061 gctgggcgtc gcgttctcag tcggcggccg agtcggagcc agcaggttgc ggccgacgac
     3121 gatgccggtg gccgcggagg ccgccgcgag gccggtgccg atgaccacct gccgacgggt
     3181 gcccgacggc ccccacggcg accggttctc ggcctgctgg gtctcaccca tctgctcggc
     3241 caaccggcga tgcaggtcgg tgatgaactc ctcacgcggc aagtcgctgc ccgtgcgggc
     3301 ggcgcgcatc tcgatggccg tcttcatctc ttgcgcgtcg agatcgtcgg ggcgtgcccg
     3361 cccggtgcgc tggccccgca gcaggccgtc gacgaagcgg cgcactggtc gtccgctcac
     3421 agcgcctcct cactgattcg cgcagccgtc tgtagtgccc gatgctgcaa caccttggc
//
LOCUS       DASYLX010000150         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1543258,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000150 DASYLX010000000
VERSION     DASYLX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543258"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1078
                     /locus_tag="VGP27_03615"
     CDS             29..1078
                     /locus_tag="VGP27_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850438.1"
                     /translation="MLRVTLRDLQWRRRRFVITIVGTSLVFAMTLLLTGLSNGFRVEA
                     NNTVDALGFDVYLAKAGPAGPFIGSPPFPESEVALVERIPGVISAVPLVFTTTTAPEG
                     DSTRNVNLFGVPAQGPGMPAIATGRAPSGVDEAAVSSTLGTDVGGQIEIGSRSMRIVG
                     IVEDSTALAGQPNVFLTVPGAQLDGFSGQPIVSSIGVHGAPAKAPDGYRIVDRAGAVG
                     DLMRPMNAAEGALTMLSVLLWIVAALIVGSVIYLSALERTRDFAVLKAVGVSTRSVLA
                     GLCLQAVIIAVLAAVLGGLLSLVLVPVFPMRVVVSTSAFLLLPVTAVLIGLLASAAGL
                     RRAVSIDPALAFGGP"
     gene            1082..2074
                     /locus_tag="VGP27_03620"
     CDS             1082..2074
                     /locus_tag="VGP27_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850439.1"
                     /translation="MGNLHINDLVVEYSSGGYAVRPIDGLNLEVSAGSLALLLGPSGC
                     GKTTLLSCLGGILRPTSGRIVLDDLDVTTLDSRELTMYRRRTVGIVFQAFNLLPSLTA
                     FENVMVPMEAAGVNRREARQRAEELLARVGLQDRMRHRPGDLSGGQQQRVAVARAIAL
                     DPPLILADEPTAHLDFIQVEEVLRLIRELASGDRLVVVATHDTRMLPLADRVVELVPQ
                     VASTNRPPETVRLTAGSVLFEQGSIGDLIYIVREGELDIFRELTGGGEELLRIANPGD
                     YFGEIGTLFGLPRSATVRARTDAVLVGYTGRAFRERLGPAGVRDLIEHRELVIN"
BASE COUNT          316 a          676 c          702 g          401 t
ORIGIN      
        1 gctcggccca tgtcggtagc gtcaccacat gctgcgtgtg acgctgcgcg acctccaatg
       61 gcgacggcgg cgcttcgtca tcacaattgt gggcaccagc ctggtgttcg cgatgacgct
      121 tcttctcaca gggttgtcta acggctttcg cgtggaagcc aacaacacgg tcgacgcact
      181 gggtttcgat gtttacctcg ccaaggccgg gcccgctgga ccgttcatcg ggtcgccgcc
      241 gtttcccgaa tccgaagtcg ctttggtcga acggattcca ggcgtgatca gcgccgtgcc
      301 gctggtgttc acgacgacca ccgccccgga gggtgactcg actcgcaacg tgaacttgtt
      361 cggcgtgccc gcgcagggcc ccgggatgcc cgcaatcgcg acgggccgcg ctccatcggg
      421 cgtcgatgag gccgcggtgt ccagcacact cggcacggat gtcggcggcc agatcgaaat
      481 aggctcgcgc tcaatgcgaa tcgtcggcat cgtcgaagac tccaccgctc tcgccggaca
      541 acctaatgtg ttcttgacgg tgccaggtgc gcaattggac ggtttcagcg gtcaacccat
      601 cgtctcgtcg atcggcgttc acggagcgcc cgcgaaggca cccgacggct accgcattgt
      661 cgaccgcgca ggcgccgtgg gcgacctaat gcggccgatg aacgcggcgg agggcgcgct
      721 cacaatgctg tccgtgctgc tgtggattgt cgccgcgctc atcgtcggct cggtcatcta
      781 cctttccgcc ttggaacgga cccgggactt cgcggtgctc aaagcggttg gcgtttcgac
      841 gcgttcggtg ctcgccgggc tttgcctaca ggcggtcatc attgccgtgc tcgccgcggt
      901 actgggtggc ctgctgtctt tggtgctcgt gccggttttc cccatgcgcg tggtggtgtc
      961 gacatcggca tttctgttgt tgcctgtgac cgcggtgctg atcggcctgc tggcgagcgc
     1021 cgcggggttg cgacgtgcgg tgagcatcga tcccgcattg gcattcgggg ggccgtgacc
     1081 gatgggtaac ctgcacatca acgatttggt ggtcgagtac tccagcggcg ggtacgcagt
     1141 ccgtccgatt gacggcctca acctcgaggt gtccgccggc tcgctggcgc tgctgctcgg
     1201 cccgagcggc tgcgggaaga ccacgctgct gtcgtgtctc ggcggaatcc tgcgtccgac
     1261 ttcgggccgc atcgtgctcg atgacctcga cgtgaccacg ctcgactcac gcgagctgac
     1321 gatgtaccgg cgccgcacgg tcggaatcgt gttccaggcg ttcaacctgc tcccgagcct
     1381 tactgcgttc gagaacgtga tggtgccgat ggaggcggcg ggtgtgaacc gtcgagaggc
     1441 gcggcagcgg gccgaagagc ttctggcacg ggtgggtctg caggaccgga tgcggcaccg
     1501 accgggcgat ctgagcggag gacagcagca acgggtcgcc gtcgcgcgtg cgatcgccct
     1561 ggatccgccg ttgatcctgg ccgacgagcc caccgcgcac ctggacttca tccaggtcga
     1621 agaggtgctg cggctgatcc gcgaactggc gtcgggagac cgcctcgtcg tcgtcgccac
     1681 ccacgacacc cggatgctcc cactggccga ccgcgtcgtg gaattggtac cgcaggtcgc
     1741 gtcgaccaac cgtccgcccg aaacagtgcg actcaccgcc ggttcggtgc tattcgagca
     1801 gggctcgatt ggcgacctca tctatatcgt cagagagggc gaactcgaca tcttccgcga
     1861 attgaccggt ggcggcgagg aattgctgcg catcgccaac ccgggtgatt acttcggcga
     1921 gatcggcacg ctgttcgggt tgccccgctc ggcgaccgtg cgggcacgga ccgatgcggt
     1981 gctggtcggc tataccgggc gagctttccg cgagcgtctc ggaccggccg gagtgcgtga
     2041 cctgatcgag caccgcgaac tcgtcatcaa ctaatccggc cggagcgcca gccgc
//
LOCUS       DASYLX010000151         7538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3737559,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000151 DASYLX010000000
VERSION     DASYLX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737559"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VGP27_03625"
     CDS             complement(<1..216)
                     /locus_tag="VGP27_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV7850440.1"
                     /translation="MSSPSPAEGRGPRAPVGIFDSGVGGLTVARAIIDQLPDEDIVYV
                     GDTGNGPYGPLTIPEIRAHALAIGDDLV"
     gene            complement(213..893)
                     /locus_tag="VGP27_03630"
     CDS             complement(213..893)
                     /locus_tag="VGP27_03630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV7850441.1"
                     /translation="MSGLGYATPATPKKRPAWVVGGVTVVSFVALLYVIELWDSLTDH
                     SLDRNGIRPLETDGLMGIVFAPLLHSNWDHLIANTIPALILGFLMTLAGLSRFIFATA
                     IIWVLGGLGTWLIGNVGTHCPYVGVSCETNHIGASGLIFGWLAFLIVFGFFTRKVWEI
                     VVGVVVLFIYGGVLLGMLPGTPGVSWQGHLCGAVAGVVAAYLLSGPERKAREARKRGA
                     PLPQTFGT"
     gene            complement(922..1893)
                     /locus_tag="VGP27_03635"
     CDS             complement(922..1893)
                     /locus_tag="VGP27_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HEV7850442.1"
                     /translation="MTRYDSLLEALGNTPLVGLRRLSPRWDDGDGAPHVRLWAKLEDR
                     NPTGSIKDRPALRMIEQAERDGLLEPGATILEPTSGNTGISLAMAALLKGYTMICVMP
                     ENTSIERRQLLELYGARIIFSPAEGGSNTAVAHAKELAAQNPDWVMLYQYGNPANTES
                     HYLGTGPELLADLPEITHFVAGLGTTGTLMGTGRYLREQVPDVQIVAAEPRYGEGVYA
                     LRNIDEGFIPELYDADVLTTRFSVGSFDAVKRTRELVQVEGIFAGISTGAVLHAALGM
                     AAKAVKAGERADIAFVVADAGWKYLSTGAYAGSLDEAEDALEGQLWA"
     gene            complement(1904..2185)
                     /locus_tag="VGP27_03640"
     CDS             complement(1904..2185)
                     /locus_tag="VGP27_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV7850443.1"
                     /translation="MTVTVSVPTILRSHTGGEKRVSATGKTLQAVIADLEANYSGISD
                     RLVDPANPGKLHRFVNIYVNDEDVRFSGGLDTEISDGDSVTILPAVAGG"
     gene            complement(2206..2619)
                     /locus_tag="VGP27_03645"
     CDS             complement(2206..2619)
                     /locus_tag="VGP27_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV7850444.1"
                     /translation="MLLVRADLVDAMVAHARADHPDEACGVLAGPEGSDRPERHVAML
                     NAERSPTFYRFDSMEQLKVWRALEDGGEAPIVIYHSHTATEAYPSRTDISYASEPDSH
                     YVLVSTRDPDVHELRSYRIVDGVVTEEPVKIVEQY"
     gene            complement(2624..3655)
                     /locus_tag="VGP27_03650"
     CDS             complement(2624..3655)
                     /locus_tag="VGP27_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV7850445.1"
                     /translation="MSAITDVGGIRVGQHERIDPDATLGSGWASGTTVVLTPPGTVGA
                     VDCRGGAPGTRETDLLDPINSVRHVDAVVLSGGSAYGLAAADGVMQWLEQQGRGVAMD
                     GGVVPIVPAAVVFDLPVGGWDCRPTAEFGNAAAHAASTKVAVGSVGAGTGARVGVLKG
                     GVGTASVTLTDIGVTVGAIVVVNAAGDAVDPRTGLPWQADQIEEFGLVSPGADDIAAY
                     ADRHTEFSPLNTTIAVVATDAAMSAAACRRVAVAAHDGLARTIRPCHTPLDGDTVFAL
                     ATGAVEVPPDPETPASMSPEVPLITAVGAAAADVLARAVLVGVLAAESIAGIPTYRDM
                     LPGAFAGKG"
     gene            complement(3652..4233)
                     /locus_tag="VGP27_03655"
     CDS             complement(3652..4233)
                     /locus_tag="VGP27_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HEV7850446.1"
                     /translation="MRKWKRVDTADGPRFRSALASHEAALLESLVTSMVAMLEERESS
                     SPADELEEITGLKTGNSRPPEDETMKRLLPDFFRPQNDHPAGSGTAESLNGALRSLHE
                     PDIIAAKREAAQRVLDTVPNGGGRFELTENDAHAWAAAVNDVRLALGTMLDISPEGPE
                     RVPTDHPMAGHFDVYQWLTVLQEYLVLGLMDKR"
     gene            complement(4256..4558)
                     /gene="clpS"
                     /locus_tag="VGP27_03660"
     CDS             complement(4256..4558)
                     /gene="clpS"
                     /locus_tag="VGP27_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEV7850447.1"
                     /translation="MVTPAKARPGTREERHVETAEDTESPWVTVVWDDPVNLMTYVTY
                     VFQKLFGYDEPHATKLMLQVHNEGKAVVSAGSRESMEVDVSKLHAAGLWATMQHDR"
     gene            4612..5916
                     /locus_tag="VGP27_03665"
     CDS             4612..5916
                     /locus_tag="VGP27_03665"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV7850448.1"
                     /translation="MTAALLTDKYELTMLAAALHDGTAHRRTTFEVFARRLPEGRRYG
                     VVAGTARFVEALSEFTFDDTALTSLSDFLDDDVLAYLADYRFRGDVDGYAEGELYFPG
                     SPVLAVHGTFGECVVLETLALSIFNHDTAIASAAARMASAAEGRTLIEMGSRRTHEQA
                     AVAAARAAYLAGFAGSSNLEAQRRYGVPALGTSAHAFTLLHTSAQGPDEKAAFRAQVD
                     ALGVGTTLLVDTYDITQGVANAVEVAGPELGAVRIDSGDLGVLARLVRAQLDGLGATK
                     TRIVVSGDLDEFAIAGLRAEPVDIYGVGTSVVTGSGAPTANMVYKLVEVDRMAVEKRS
                     LHKESHGGRKRAMRLAKPSGTVVEEIVYPAAGNPPQSDLVARDLTVPLVKNGEPVADL
                     SLASARDRVAAGLHSLPWDGLKLSRGEPAVATRMVPPACRGA"
     gene            5925..>7538
                     /locus_tag="VGP27_03670"
     CDS             5925..>7538
                     /locus_tag="VGP27_03670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV7850449.1"
                     /translation="MSDEAPVTELLATAVAALGGSERSGQVQMAEAVAHAFDAGEHLA
                     VQAGTGTGKSLAYLVPAIARAIAADEPVVISTATIALQRQLVDRDLPALADSLAEALP
                     REPKFALLKGRGNYLCLNKIHNGAAAEPDDRPQEELFDAAASTALGRDVARLIAWSSN
                     TDTGDRDELAPGVPDRSWGQVSVSSRECIGAARCPFGTDCFAEKARSRAAEVDVVVTN
                     HALLAIDAISDAAVLPEHRLLVVDEAHELADRVTGVATAELSNAILSAATRRVARLVE
                     PELTQRLEAATATFASAIHDATPGRIDRLDDEMTTYLTALRDAASRVRSAIDMSPSDP
                     KAASARTEAVTALSDISDTATRIVASFVPEIPNRDDVVWLDHEDSAHARSNKGGGGAI
                     RAVMRVAPLSVAGLLRGRLFDQSTVVLTSATLTVGGSFDAMAAAWGLTGGDEQPDWRG
                     IDVGSPFEHAKAGILYVAAHLPPPGRDSTGSAEQLDEIQTLIDAAGGRTLGLFSSMRA
                     ARAAAEIMRERLSTPVLCQGDDTTSALVKQ"
BASE COUNT         1185 a         2584 c         2568 g         1201 t
ORIGIN      
        1 gacgaggtcg tcgccgatcg ccaaagcgtg cgcccggatc tcggggatgg tcagtgggcc
       61 gtaggggccg ttgccggtgt ctccgacgta gacgatgtcc tcgtcgggca gttggtcgat
      121 gatcgcccgc gcgacggtga gtccgccgac gccggagtcg aagatgccga caggcgctcg
      181 aggcccccgt ccctcggcgg gggacggcga gctcacgtgc cgaaggtctg tggcagaggg
      241 gcgccacgct tacgggcctc gcgggccttt cgctcgggcc ccgacaacag gtacgcggcg
      301 accacgccag cgaccgcgcc gcacagatgc ccctgccagg acactcctgg agtgccgggc
      361 agcatgccga gcagcacccc tccgtagatg aacagcacga cgacgccgac cacgatttcc
      421 cataccttgc gggtgaagaa accgaacacg ataaggaacg ccagccagcc gaagatcagg
      481 cccgacgcgc cgatgtggtt ggtctcgcag ctgaccccga cgtacgggca gtgcgtgccg
      541 acgttgccga tgagccatgt gccgaggcca ccgaggaccc agatgatggc cgtcgcgaag
      601 atgaatcgcg acagacccgc gagcgtcatc aggaagccga ggatcagtgc cgggatggtg
      661 ttggcgatga ggtggtccca gttcgagtgc agaagcggcg cgaagacgat gcccatcaag
      721 ccgtcggtct ccagcggcct gatgccgttc cgatcgaggc tgtggtcggt caggctgtcc
      781 cacagctcga tgacatagag cagcgcaacg aagctgacga cggtgacgcc gccgaccacc
      841 cacgccgggc gcttcttcgg cgtcgccggc gtcgcatagc ccagcccgct caagcgcact
      901 cgattcgctc gcaagcttcg ctcatgccca tagctgccct tccaaagcgt cttccgcttc
      961 gtcaaggcta ccggcgtatg cgccggtcga cagatacttc cacccggcgt cggccaccac
     1021 gaacgcgatg tcggcccgct ccccggcctt caccgccttc gccgccatac cgagcgcggc
     1081 atgcagcacc gcgcccgtcg aaatgccggc gaagatgccc tccacctgaa cgagttcgcg
     1141 ggtgcgtttg accgcgtcga acgatccgac cgagaaccgc gtcgtcagca cgtcggcgtc
     1201 atacaactcg gggatgaacc cctcgtcgat gttgcgcagc gcgtagacac cctcgccgta
     1261 gcggggctcg gcggccacga tctggacgtc gggcacctgc tcgcgcagat accgtcccgt
     1321 gcccatcagc gtgccggtgg tgccgaggcc cgcgacgaag tgcgtgatct ccggcaggtc
     1381 ggccagcagc tcgggtccgg tgccgaggta gtgcgattcg gtgttggccg ggttgccgta
     1441 ctggtagagc atcacccagt ccgggttctg cgcggccaat tccttggcat gcgccaccgc
     1501 ggtgttcgag ccgccctcgg cgggcgagaa gatgatccgc gcgccgtaga gctcgagcaa
     1561 ttggcgacgc tcgatcgacg tgttctccgg catcacgcag atcatcgtgt agcccttgag
     1621 cagggcggcc atcgccagcg agatgcccgt attgccactg gtcggctcca ggatcgtggc
     1681 accgggttcg agcaacccgt cgcgctcggc ctgttcgatc atccgcagcg cgggccggtc
     1741 cttgatcgag cccgtcgggt tgcggtcctc gagcttggcc cacagccgga cgtgcggcgc
     1801 cccgtcgccg tcgtcccagc gcggcgacaa ccggcgcagg cccaccagcg gcgtgttgcc
     1861 gagcgcctcc agcagtgagt cgtaccgcgt catcggcgcc tactcaccct ccggccacgg
     1921 cgggcaggat cgtgaccgag tcgccgtcag agatttcggt gtcgaggccg ccggagaagc
     1981 gcacatcctc gtcgttgacg tagatgttga cgaagcggtg cagcttgccc ggattagccg
     2041 ggtcgacgag gcggtcggag atgccggagt agttggcctc cagatcggcg atgacagcct
     2101 gcagcgtctt gcccgtcgcg gagacgcgtt tctcgccgcc ggtgtggctg cgcagaatgg
     2161 tgggcacgga aacggtaacg gtcatgggtg gcctctcttg gacgtctagt actgctcgac
     2221 gatcttgacg ggttcctcgg tgacgacgcc gtcgacgatg cggtagctgc gcagctcgtg
     2281 cacgtcgggg tcgcgggtgg acacgagcac gtagtgcgaa tcgggttcgg atgcatacga
     2341 gatgtcggtg cggctgggat aggcctctgt cgcggtatgc gagtggtaga tgacgatcgg
     2401 agcctcacct ccgtcctcca acgcccgcca caccttcagc tgctccatcg agtcgaaccg
     2461 gtagaaggtc ggcgaccgct cggcgttgag catcgcgacg tgccgctcgg gacggtcgga
     2521 cccctcggga ccggcgagca caccgcacgc ctcgtcggga tggtcggcac gggcgtgcgc
     2581 gaccatggcg tcgaccaggt cggcgcgaac cagcagcaca gtcctacccc tttccggcga
     2641 acgctccggg caacatgtcc cggtatgtcg gtattccggc tatcgactct gccgcaagaa
     2701 caccgacgag caccgcccgg gccaggacat ccgccgcggc ggcccccacc gcggtgatca
     2761 gcggcacctc cggcgacatg gaggccggcg tctcgggatc cggcggcacc tcgacggcac
     2821 cggtcgccag cgcgaacacc gtatcgccgt ccagtggggt gtgacacggg cgaatggtgc
     2881 gggcgaggcc gtcgtgggcg gcgacagcga cgcggcggca cgcggccgcg ctcatcgcgg
     2941 cgtcggtggc gaccaccgcg atcgtggtgt tgagcgggct gaactcagtg tgccggtcgg
     3001 cataggccgc gatgtcgtcg gctcccggcg agacgagccc gaactcctcg atctggtcgg
     3061 cctgccacgg cagtccggtc ctcgggtcga cggcgtcgcc agcggcattg acgacgacga
     3121 tcgcgccgac cgtgacaccg atgtcggtga gggtcaccga cgcagtgccg acgccgccct
     3181 tcagcacgcc gacgcgtgcg ccggtgcctg cgccgacgga gccgactgcc accttcgtcg
     3241 acgcggcgtg tgcggctgcg ttgccgaact ccgcggtggg cctgcagtcc caaccgccga
     3301 cgggcaggtc gaataccacc gcagccggga cgatcggcac gactccgccg tccatcgcca
     3361 cgccgcgacc ctgttgctcc agccactgca tcacgccgtc ggccgccgcc aggccgtagg
     3421 cgctgccgcc gctgaggacg accgcgtcga cgtggcggac cgagttgatc gggtcgagca
     3481 ggtcggtctc cctcgtgccc ggcgcgccac cgcggcagtc gacggcaccg acggtgccgg
     3541 gcggggtcag cacgaccgtc gtgccgctgg cccagccgga gccgagggtc gcgtcggggt
     3601 cgatgcgctc gtgttggccg acccggatac cgccgacgtc ggtgatggcg ctcatcgctt
     3661 gtccatcagg ccgaggacca gatactcctg cagcaccgtc aaccactggt acacgtcgaa
     3721 atgaccggcc atcgggtggt cggtcggcac ccgttcgggt ccctcggggc tgatgtcgag
     3781 catcgtgccc aaggcgagcc ggacgtcgtt gaccgcggcc gcccacgcat gcgcatcgtt
     3841 ctcggtcagc tcgaatcgac cgccgccgtt cggcaccgtg tccaaaaccc gttgcgccgc
     3901 ttcgcgtttg gcggcgatga tgtccggctc gtgtaggctg cgcagcgcgc cgttcaggct
     3961 ttccgccgtg cccgacccgg cgggatggtc gttctgcggc cggaagaagt cgggcagcag
     4021 cctcttcatc gtctcgtcct caggaggacg ggagttaccg gtcttgagcc cggtgatctc
     4081 ctcgagttcg tctgcgggcg atgatgattc gcgctcttcg agcatcgcga ccatagacgt
     4141 caccaggctt tcgagcagcg ccgcctcatg cgaggccaac gccgaacgga agcgcgggcc
     4201 gtcagcggtg tcgacccgtt tccactttcg cactatcgcg tcccggctcc gtctatcagc
     4261 ggtcgtgttg catggtcgcc cacaggccgg cggcgtgcag cttggatacg tcgacttcca
     4321 tggactcgcg gctgccggcc gacaccaccg ccttgccttc gttgtgcacc tgcagcatga
     4381 gcttggtggc atgcggctcg tcgtaaccga acagcttctg gaagacgtag gtcacgtacg
     4441 tcatcaagtt gacagggtcg tcccagacga cggtcaccca cggggactcg gtgtcctcgg
     4501 cagtctcgac gtgtcgttcc tcgcgagttc ccggccgggc cttcgctggg gtaaccatgt
     4561 gcccagaata gccggggccc gatagggccc gagaacggtt acggttgacc gatgacagca
     4621 gcgctgctga ccgacaaata cgagctcacc atgcttgccg cggcgctgca cgacgggacc
     4681 gcgcaccggc gcacaacttt cgaggtgttc gcgcggcggc tgccggaggg caggcggtac
     4741 ggcgtggtcg ccgggaccgc gcggttcgtg gaagcgttgt cggagttcac gttcgatgac
     4801 accgcgctga cgtcgctgtc ggacttcctc gacgatgacg ttctggcgta cctcgccgac
     4861 taccgattcc gcggcgacgt cgacggttat gccgaaggtg agttgtactt tcccggttcg
     4921 ccggtgttgg cggtacacgg cacgttcggc gagtgcgtcg tactcgagac actggccttg
     4981 tcgatcttca atcacgacac tgcgatcgca tcggccgcgg cccggatggc gagcgccgcc
     5041 gagggccgga ccctcatcga gatgggttct cggcgcaccc acgagcaggc cgccgtcgcc
     5101 gccgcgcgcg cagcctatct tgcgggtttc gccggttcgt cgaatctcga ggcccagcgc
     5161 cgctacggcg tgcccgcact gggcaccagc gcgcacgcgt tcacgttgct gcacacctcc
     5221 gcccaagggc cggacgagaa ggcggcgttt cgcgcccagg tcgatgccct cggcgtgggc
     5281 accaccctgc tcgtcgacac ctacgacatc acccagggcg tcgccaacgc cgtcgaggtg
     5341 gccggccctg aactcggcgc cgtccgcatc gactccggcg accttggcgt gctggcccgg
     5401 ctggtccgtg cccaactcga cggcctcggc gctacgaaga cccgcatcgt cgtctcgggt
     5461 gacctcgatg agttcgccat cgcggggctg cgcgccgaac ccgtcgacat ctacggcgtc
     5521 ggcacctcgg ttgtcaccgg ctccggcgcc ccgaccgcga acatggtcta caagctggtc
     5581 gaggtggacc gcatggctgt ggagaaacgc agcctgcaca aggagtcgca cggtggccgt
     5641 aagagggcga tgcggctggc caagccgtcg ggaaccgtcg tggaggagat cgtctacccg
     5701 gccgccggaa accccccgca gtccgatctc gtcgcacgcg atctgacggt cccgctggtc
     5761 aagaacggcg agcccgtcgc cgacctgagc ctggcctcgg cccgcgaccg cgtcgcggcg
     5821 ggcttgcaca gcctgccgtg ggacggcctg aaactgtccc gcggcgaacc cgcggtcgcg
     5881 acccggatgg ttccgcccgc ctgccgaggc gcgtaggagg agccatttcc gacgaagctc
     5941 cggtcaccga actgctcgcc accgcggtcg ccgcgctcgg tggcagcgag cgcagtggtc
     6001 aggtccagat ggccgaggcc gtcgcgcacg cattcgacgc gggtgagcat ctggccgtgc
     6061 aggcgggcac cggcaccggc aagtcgctcg cgtacctggt gcccgcgatt gcgcgcgcca
     6121 tcgccgccga cgaaccagtg gtgatttcga cggccacgat tgcgctgcaa cgccagctcg
     6181 tcgaccgcga cctgcccgcg ctcgccgact ccctcgcaga agcgctgcca cgcgagccga
     6241 aattcgctct gctcaagggg cggggaaact atctgtgcct gaacaagatt cacaacggcg
     6301 cggcggcgga gccggatgat cggccgcagg aggaattgtt cgacgcggcg gcttcgacgg
     6361 cgttgggacg cgacgtggca cgcctcatcg cctggtcgtc gaacaccgac accggcgacc
     6421 gcgacgagct cgcacctggc gtgccggacc ggtcatgggg tcaggtcagc gtctcttcga
     6481 gggaatgcat cggtgcggcg cgctgcccgt tcggcaccga ctgcttcgcg gagaaggcga
     6541 gaagccgggc cgccgaagtg gatgtcgtcg tcaccaacca cgcactgttg gcgattgacg
     6601 ccatctccga tgccgcggtg cttcccgaac atcggctcct ggtcgtcgac gaggcacacg
     6661 aactggcgga ccgggtcacg ggcgtggcca ccgcggagtt gtccaacgcg atcctcagcg
     6721 ccgcgacccg acgcgtcgcg cgactcgtcg agcccgaact gacgcaacga ctcgaggccg
     6781 cgacagcgac gttcgcgtcc gccattcacg acgcgacgcc cgggcgcatc gaccgcctcg
     6841 acgacgagat gacgacgtac ctgaccgcgc tgcgcgacgc cgctagccgc gtgcggtcgg
     6901 cgatcgacat gtcaccctcc gaccccaaag cggcgtcggc gcgaaccgag gcggtcaccg
     6961 cgctgtccga catcagcgat accgcgacgc gcatcgtcgc gtcgttcgtc cccgagattc
     7021 ccaaccgtga cgatgtggtg tggctggacc atgaggattc agcgcacgcg aggagcaata
     7081 aagggggagg cggagctatt cgggccgtca tgcgagtggc gccgctctcg gtggctggtc
     7141 tgttgcgggg caggctgttt gaccagtcca ccgtggtgct gacatcggcc acgctgacgg
     7201 tcggcggctc gttcgacgcg atggccgcgg catgggggtt gaccggcggc gacgaacagc
     7261 cggactggcg tggcatcgac gtgggatctc cgttcgagca cgcgaaggcc ggaatcctct
     7321 atgtcgcagc gcatctaccg ccgccggggc gcgacagcac cggctccgcc gagcaactcg
     7381 acgagatcca gacgcttatc gacgccgcgg gcggccgaac gctgggactc ttctcgtcga
     7441 tgcgggccgc acgggcggcc gcggagatca tgcgagagcg gctgtccaca ccggtgctct
     7501 gccagggcga cgacaccacg tccgccttgg tgaagcag
//
LOCUS       DASYLX010000152         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3408102,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000152 DASYLX010000000
VERSION     DASYLX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3408102"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..604
                     /locus_tag="VGP27_03675"
     CDS             5..604
                     /locus_tag="VGP27_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850450.1"
                     /translation="MARTQQQRREDTIARLLDASIATIIEVGYARASAKMITQRAQVS
                     DGALFRHFPTMGDLMAATAQEAGRRQLELAAKRIADIPSDRPAIEEVLTIIRDLTANS
                     TNTVIYELMVAARTDEKLCDTLREVMASYIAKIYGAARAVATADEIKAFGEENFAALV
                     AVLINTFDGAAMFRHVLTQAEIEDRRVPVLMSLLNAARP"
     gene            657..3116
                     /gene="ponA2"
                     /locus_tag="VGP27_03680"
     CDS             657..3116
                     /gene="ponA2"
                     /locus_tag="VGP27_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HEV7850451.1"
                     /translation="MSEQRAANPAKLILYVLTAGLLLAALVFPFVGGAGAVANRLSDR
                     AAQEAAELLEGQVPVISTMVDAAGNPIAWIFTQRRWVVPGNRIADTMKLAIVSIEDKR
                     FGLHNGVDVQGSLTGLAGYLQGAVDTRGGSTIEQQYVKNYNFLVGAHNDAERRAAIET
                     TPVRKLREMRMALELDKALPKAEILTRYLNLVSFGNGSFGVQDAAKTYFGVNAGDLNW
                     QQAALLAGMVQSTSALNPYTNPEGALARRNLVLDTLIENVPARADEIRAARAAPLGVL
                     PKPAPLPQGCIGAGDRAFFCDYALSYLARAGIEKDDLARNGYLIRTTLDPKVQDSVKK
                     AIDKVASPTLEGVASVMSVIKPGRDAHRVLAMGDNRTYGLELGAGQTVQPQPFTSVGD
                     GAGSVFKIFTTAAALDMGMGINAQLDVPAKFEGAGLGDSNSPGCPPRTWCVKNAGGGG
                     RGPMSVTDALAQSPNTAFAKLIAQVGVPRAVDMAVRLGMRSYAEPGSARDYDPNNKGS
                     LADYVKQQNLGSFTLGPLELNALELSNVAATLASSGMWCPPDPIDKLIDRNGREVPLP
                     QTKCEQVVPEGLANTLANALGKDHISGTAASSAGSVGWTLPMSGKTGTTESHRSSAFL
                     GFTNQYAAANYIFDDTPAPSDLCSWPLRRCSSGNLYGGNEPARTWYLAMKPIANDLGP
                     VAMPPTDPRYVNGAPTVPLPRITGLNFDAARTRLQDAGFQVADLPSPVDSYLSKGSIV
                     GTTPRDKAVPGFLITINISNGVSPAPVYYPPRRPYAPPPPPPAVLPPPDANIIGVPGL
                     PPVFLPAPPPPGPVPAAPPPP"
     gene            complement(3113..3337)
                     /locus_tag="VGP27_03685"
     CDS             complement(3113..3337)
                     /locus_tag="VGP27_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850452.1"
                     /translation="MLVVDEIGQDVHAGRKVYVWIGTVYKLEAERTGPKALFLGNMTA
                     IPMCRGVWSIPVDRQVLKGVPKAGACFDTG"
     gene            3807..>5142
                     /locus_tag="VGP27_03690"
     CDS             3807..>5142
                     /locus_tag="VGP27_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7850453.1"
                     /translation="MEILLERESALAQLNVLRRRAGGGSGRVVLLRGEAGVGKTAVIR
                     RFVAGLTGQVRVLRGACDPLATPRVLGPFVDMLAQVRDPATERLAAAIGGNDPEAIYA
                     ALLGVFGDGNTWVFAVEDVHWADGATLDVLRFLARRVGSLPVLLLVSYRDDEVGERHP
                     LAMALGDVATCAEVTRIGLDRLSREAVAELAAGGGVNADELYRLTGGNPFFVTEVLAV
                     SPDSLGYRDLPRSVSEAVWGRLARLSAPARDTAQAAAVCGPRASAQLLEKVFPGAAAA
                     LDECFDTGVLCADGAAVAFRHELARQAALEQIPGYRRRLLHARALAALAEPPIAPDTL
                     SALAFHADQAGDQDAATRYGPAAAEQAEGLGAHREAAELYALTLRHAEGTPAEQKVVW
                     LERHAFTSYMCGLAEAAARSWREAIAVRHELGDRLAEGDDLRWLSQMLWPLGR"
BASE COUNT          835 a         1708 c         1754 g          845 t
ORIGIN      
        1 tgagatggcc agaacccagc agcagcgccg agaggacacg atcgcccgtc tgctcgacgc
       61 gagcatcgcc accatcatcg aggtcggcta cgcccgcgct tcggcgaaga tgatcacgca
      121 gcgcgcgcag gtgtccgacg gcgccctgtt ccggcacttt ccgaccatgg gcgacctcat
      181 ggcggcgacc gcccaggagg cggggcgacg gcagctggag ttggcggcga agcggatcgc
      241 cgacatcccc tccgaccggc cggcgatcga agaggtgctg acgatcatcc gcgacctgac
      301 cgccaactcc accaacaccg tcatctacga gctgatggtc gccgcacgca cggatgagaa
      361 gctgtgcgac accctgcggg aggtgatggc gagctacatc gcgaagatct acggcgccgc
      421 gcgcgcggtg gccaccgccg acgagatcaa ggcattcggc gaagagaact tcgcggcctt
      481 ggtggcggtg ctcatcaaca ccttcgacgg cgccgccatg ttccgccatg tgttgacgca
      541 ggcagaaatc gaggatcgtc gggtccccgt gttgatgtcg ctgctgaatg ccgcgcgtcc
      601 ataacgatcc cgctgcattg gcgcctccgc cgtcacgctg cccataggct ggaagcgtgt
      661 cggagcagcg cgcggccaac cctgccaaac tgattctcta tgtgctgacg gcgggtcttc
      721 tgctggcggc cttggtgttt cccttcgtcg gcggcgcagg cgcggtggcc aaccggctct
      781 ccgacagggc ggcccaggag gcggctgagc tcctggaggg ccaggtgccg gtcatctcca
      841 cgatggtcga cgccgcgggt aacccgatcg cgtggatttt cacccagcgc cgctgggtgg
      901 ttcccggcaa ccgcatcgcc gacacgatga agctggccat cgtgtcgatc gaggacaagc
      961 ggttcggtct gcacaacgga gtggacgtgc agggctcgct caccgggctg gccggctacc
     1021 tgcagggcgc agtcgacacc cgcggcggtt cgacgatcga gcagcagtac gtcaagaact
     1081 acaacttcct ggtgggcgct cacaacgatg cggagcgccg ggcggcgatc gagacgacgc
     1141 cggtgcgcaa gctgcgcgag atgcggatgg cgctggaact ggacaaggcg ctgcccaagg
     1201 cggagatcct gacccggtat ctgaacctgg tgtcgttcgg caacggcagc ttcggcgtgc
     1261 aggatgccgc gaagacgtat ttcggcgtca acgccggcga cctcaactgg cagcaggcgg
     1321 cgctgctggc cgggatggtc cagtcgacga gcgcgctcaa tccctacacg aatcccgagg
     1381 gcgccttggc gcgccgaaac ctggtgctcg acacgctcat cgagaacgtc ccggccagag
     1441 ccgacgagat ccgcgccgca agggcggcgc cgttgggtgt cctgcccaag cccgcgccgc
     1501 tgccgcaggg ctgtatcggg gccggcgatc gcgccttctt ctgtgattac gcgctgtcgt
     1561 atctcgctcg tgcgggcatc gagaaggacg acctggcgcg aaacggctac ctgatccgca
     1621 ccaccctcga tcccaaggtg caggactccg tcaagaaggc catcgacaag gtcgcgagtc
     1681 ccacgctgga gggcgtcgcc agcgtgatga gcgtcatcaa gccgggcagg gacgcgcacc
     1741 gcgtgttggc gatgggcgac aaccgcacgt acgggctcga gctcggtgcc gggcagaccg
     1801 tgcagccgca gccgttcaca tcggtcggtg acggcgcggg ctcggtgttc aagatcttca
     1861 ccaccgccgc ggcgctcgac atggggatgg gcatcaacgc ccaactcgac gttcctgcga
     1921 agttcgaagg cgcgggactg ggtgacagca acagccccgg ttgtccccca cgcacgtggt
     1981 gcgtcaagaa cgcgggcggc ggcgggcgcg gcccgatgag cgtcaccgat gcgctggccc
     2041 agtccccgaa cacggctttc gccaagctga ttgcgcaggt cggtgttccg cgcgcagtag
     2101 acatggcagt gcggctgggg atgcgttcct atgccgagcc gggcagcgcc agggactatg
     2161 acccgaacaa caagggcagc ctcgccgact acgtcaagca gcagaacctc ggctcgttca
     2221 ccctcggacc actcgagctc aatgcgctcg agttgtcgaa cgtggccgcc accctggcgt
     2281 cgagcggcat gtggtgcccg cccgacccta tcgacaaact gatcgaccgg aacgggcgcg
     2341 aagtgcctct tccgcagacg aagtgtgagc aggtcgtgcc cgagggcctc gccaatacgc
     2401 tcgccaacgc gctcggcaag gaccacatca gcgggacggc agccagctcg gccggttccg
     2461 tcggctggac cctgccgatg tcggggaaga cgggcaccac cgagtcgcat cgctcgtcgg
     2521 cgttcctggg gttcaccaac cagtacgccg ccgcgaacta catcttcgac gacacccccg
     2581 cgccatccga cctgtgctca tggccactgc gcagatgctc gagcggaaac ctctacggcg
     2641 gcaacgaacc tgcgcgtacg tggtacctcg cgatgaaacc gatcgccaat gacctcggcc
     2701 cggtggcgat gcccccgacc gatccgcgct acgtgaacgg cgcgccgacc gttccgctgc
     2761 cgcgcatcac gggtctgaac ttcgatgccg cgcgtacacg cctgcaggat gccggtttcc
     2821 aggtcgccga cctgccgtcg ccggtggaca gctacctgtc gaagggatcg attgtcggca
     2881 ccacgccgag ggacaaggcc gtgcccggct tcctgatcac gatcaacatc agcaacggcg
     2941 tctcgcccgc gccggtgtac tacccgcccc gccggccgta cgcaccgccc ccgccgcctc
     3001 ccgctgtgct gccaccgcct gatgccaaca tcatcggcgt cccaggattg ccgccggtat
     3061 tcctgcctgc gccaccgccg ccggggcctg tgcccgccgc accacctccg ccttagccgg
     3121 tgtcgaagca cgcaccggct ttcggtaccc ctttcagcac ttgccgatca accggaatac
     3181 tccagacgcc gcgacacatc gggatggccg tcatgttgcc gaggaacagc gccttggggc
     3241 ctgtcctttc agcctcgagc ttgtaaaccg tgccgatcca gacgtacacc ttgcgtcctg
     3301 cgtgaacgtc ctgaccgatc tcatcgacga cgagcatcag ctcaccgtgt gccgtctggg
     3361 tccggaaaac ctgatccccc acgacgatcg attcggccgg caccgccttc tgtgctggtt
     3421 gtacaaacat cgttgccaac accgatcagc ccgcatccga ttcgaggcgg tgcgggttag
     3481 gcagtgatgg ccggcgtggc gcgggtcgct tttcctgcgg cctccccggc catcacttgg
     3541 cagagcgcac aagcgccgcc cagatccccg tagccgtcac ttaggtacct ggtgcctgcc
     3601 cgaagccccg gagaaatagc gtcaaacaca atctctggaa ctccacccct cccccagggg
     3661 taattgcagt ctggctggcg gccggtccga gtacatgagg aattttcccc atattgcggt
     3721 ttcggcccgc gggatatcgt tggtcccggc ccgcgcgaca gcgtcggcgc gaatccagcc
     3781 gagggtgaag gggcatccga gcgcctatgg agatcctgct ggagcgggag tcagcgctgg
     3841 cgcagttgaa tgtgttgcgg aggcgggccg ggggtggttc cgggcgggtg gtgctgctgc
     3901 gcggggaggc cggggtcggc aagacagcgg tgattcgccg ctttgtcgcc gggttgaccg
     3961 ggcaggtgcg ggtattgcgc ggcgcgtgtg atccgttggc gacgccgcgg gtgctgggac
     4021 cgttcgtcga catgctggcg caggtgcgcg acccggcaac ggagagattg gccgctgcga
     4081 tcggcggcaa tgacccagag gcgatctacg ctgcgctctt gggcgtgttc ggtgacggca
     4141 acacatgggt gttcgccgtc gaggacgtgc actgggccga cggcgccacc ctggacgtgc
     4201 tgcggttcct ggcgcggcgg gtggggtcgc tgccggtgtt gttgctggtt tcctaccgcg
     4261 acgatgaagt cggcgagcgg catcctctgg cgatggcgct tggtgatgtg gcgacctgtg
     4321 cggaggtgac ccggatcggg ttggaccggc tcagccggga ggcggtcgcg gagttggccg
     4381 ctggtggcgg tgtcaacgcc gatgagttgt accggctcac cggcggcaac ccgttcttcg
     4441 tcaccgaggt cttggccgtc agccccgact cgttgggata ccgcgattta ccgcgcagcg
     4501 tttccgaggc ggtgtggggg cggctggccc gactgtcggc acccgcacgt gataccgcac
     4561 aggccgcggc ggtgtgcggc ccgcgcgcca gcgcgcagct gcttgagaag gtgtttccgg
     4621 gcgcggcggc agcgttggat gaatgctttg acaccggggt gttgtgcgcc gacggggcgg
     4681 cggtggcttt tcgccacgag ctggctcgcc aggccgcgct ggaacagatc cccgggtacc
     4741 ggcgccggct gctacatgcg cgcgcactag cggcgctcgc tgaaccgccc atcgccccgg
     4801 acacgttgtc ggcgttggcg tttcatgccg atcaagccgg tgatcaggat gcggctaccc
     4861 gctacgggcc agccgccgcc gaacaggccg aggggttggg cgctcaccgg gaggccgcgg
     4921 agctctacgc gttgacgctg cgccacgccg agggcacccc tgccgagcag aaggtggtgt
     4981 ggctggaacg acacgcgttc accagctaca tgtgcgggct cgccgaagca gctgcgcggt
     5041 cctggcgcga ggcgatcgcg gtgcgccacg agctgggtga ccggctcgca gagggcgatg
     5101 atctgcgctg gctgtcacaa atgctgtggc cgttgggtcg ta
//
LOCUS       DASYLX010000153         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_336029,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000153 DASYLX010000000
VERSION     DASYLX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336029"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGP27_03695"
     CDS             <1..744
                     /locus_tag="VGP27_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850454.1"
                     /translation="STVQLDLTSLGSVRAAAATIVNITPVIHVLMNNAGVMFTPFSRT
                     SDGFEMQFGTNHLGHFELTRLLVPQLLAAGGARIVILSSDGHLMSGVDLDDPNWERRD
                     YDKFVAYGASKTANILHMVELDRRLRDGGVRAYAVHPGVVATSLARHMTREDFAKLSR
                     YATMEPEAAKEVIDVRHDFTTPDHGAATQVWAAVSPELAEVGSVYLADCRIREDVAAH
                     AADQTHALRLWELSEELCGNRAGVTGARP"
     gene            750..1325
                     /locus_tag="VGP27_03700"
     CDS             750..1325
                     /locus_tag="VGP27_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850455.1"
                     /translation="MDRRKMEVRDRILAAAFDAFLLQGVSATTIEDICERADVANRTF
                     FNHFPTRQDMMRALAERRLLNLHDVIFDRSTEPIPARLIGVFDDIAAALVKSGDTYRE
                     LIGEMLGSAGYGIQRGFALHETFLELVKDGVARGEVGVRHDAQTLADIIVGALTGGIV
                     NWTADSTYSLETGLHDLAVALADLLVGAEDA"
     gene            1422..2252
                     /locus_tag="VGP27_03705"
     CDS             1422..2252
                     /locus_tag="VGP27_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7850456.1"
                     /translation="MSPDLTVAISDGVAVLTLNRPDHLNAYTGEMGTLLGQAYRSCDE
                     DDDVRAIVLTGAGQAFCAGADFSGGADPFGSPGDASCFSASPIDPPAFELRTPVIAAL
                     NGHAIGIGLTLALQADIRIVAAEAKYGVVQVRRGVIGDCMSHWTLPRLAGVAVAAEML
                     LTGRTFDGIEAVARGIATRAVPSGEVVDHAMEIARDIAVNVAPLSAALSKRLLWESLN
                     NGYSAREVAWMETQLHHRVMGRDDAREGVAAFLGRRPPRWSGSLCRDWAELPHPPAVT
                     "
     gene            2288..3142
                     /locus_tag="VGP27_03710"
     CDS             2288..3142
                     /locus_tag="VGP27_03710"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV7850457.1"
                     /translation="MHIDLLAGGLGFTEGPVWLDDRRIALTSISHGCIYIVDPSGGAT
                     ERIETGGGPNGLARGSDGTLFVAQNGGVFGASGPAQPGVQVITDGRVEYLVTGLGAPN
                     DLMFGPDGRLWVTDTRCEIDPVSPGDGLPGQVWAVDVVTGHTELIVVSGPVFINGLGF
                     TRDGRTLLVTATLAAELLAYRLAPADAASWRQPRLVHTFDNGWPDGIAISARGETWVA
                     LTAADRVDVINDAGERTASITMPPGSLPTNICIGVGHANELFVTAAHAESLLRIRLDE
                     RGDPRLSP"
     gene            3250..4686
                     /locus_tag="VGP27_03715"
     CDS             3250..4686
                     /locus_tag="VGP27_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850458.1"
                     /translation="MALTEQSAPSVRDFMDDPIGFFGESLTQMHSIPRAELEQLQRNA
                     MGIRFQQHRESIEMLRKLADRLDITELSDLNDVVPLFFSHTAFKSYPAALIDNKRFDL
                     MTKWLDKLTSHDLSAVDTQGCNSIDEWIDRLDDQTPLEVITSSGTTGTLSILPKDKRG
                     AREGMVLWKICLFQTFGVEPTAEQLNPAVEVIWPNYASGKLGHLRIANMLKGGFTGGD
                     ESRFHALYSDSIDTDLMFLASKMRAAASRGELDRLVVDPALAARKDEFIAMQMRQPQE
                     LDVFFSEMSEQLRGKRVFMIGTYNLMYDVAKAALERGIKGVFAEDSVILTGGGSKGFV
                     LPDGYLDTIREFLGVDRIQEGYGFSEQSAFHFACTEGRYHVQPWVIPFVLDPETSRPL
                     PREGRQTGRAAVYDVLLKAHWGGVISGDEVTIDWDLACPCGQTSVAFEHDIIRYSEKR
                     GVEDDRITCAATHEVHNEAVNFMKEFES"
     gene            4683..>5341
                     /locus_tag="VGP27_03720"
     CDS             4683..>5341
                     /locus_tag="VGP27_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEV7850459.1"
                     /translation="MSAAFTVPLFLRGQIITDDLVSFGIRGGGAEFQSPDMSKYVDRL
                     PLASPMEMADLYDIGFDEILDVLEAVGDALAFERNAHLQEAYEAAILANPLPAVMLMN
                     SYKILQPLFARANVLEVADSQVGLDYLNGWVPQRLADGRELRVRAFGSRVLHIPAGNG
                     GLVSAVTILRSVIARCDTIIKAPSNDPLTAMAIARTVADVAPDHPITKHLAVGYWKGG
                     DL"
BASE COUNT          962 a         1713 c         1723 g          943 t
ORIGIN      
        1 tcgacggtcc agctcgacct gacctcgctg ggcagcgttc gcgcggcggc ggcgacgatt
       61 gtcaacatca ccccggtgat ccatgtcctg atgaataacg ccggggtgat gttcacgccg
      121 ttcagtcgga cgtctgacgg cttcgaaatg cagttcggga caaaccatct ggggcatttc
      181 gaactcaccc ggctgctggt cccgcagctc ctcgctgccg gcggtgcgcg gattgtgatt
      241 ctttcgtcgg acggccacct gatgagtggc gtcgacctcg acgaccccaa ctgggaacgg
      301 cgggactacg acaagttcgt cgcgtacggg gcttcgaaga ccgccaacat tctgcacatg
      361 gtcgaactgg accggcgact gcgcgacggc ggcgtccggg cgtacgcggt gcaccccggc
      421 gtggtcgcaa cgtcgctggc tcggcacatg acgcgcgaag acttcgcgaa gctgagccgg
      481 tacgccacca tggaacccga agcggccaag gaggtcatcg acgtgcggca cgacttcact
      541 acgcccgatc acggggcggc cactcaggtg tgggccgcgg tgagcccaga actggcggag
      601 gtcggatccg tctatctcgc tgactgccgg atccgcgagg acgtcgcggc gcacgctgct
      661 gaccagacgc atgctctccg gctctgggaa ttgtccgagg agttatgcgg caaccgtgcc
      721 ggcgtcacag gggcgcggcc gtgaacagga tggaccggcg caagatggag gtccgcgacc
      781 ggatactcgc agcggcattc gacgcatttc tgctccaggg cgtctctgcc accaccatcg
      841 aggacatctg cgaacgggcc gacgtcgcga atcggacgtt cttcaaccac ttcccaacgc
      901 ggcaggacat gatgcgtgcc ctcgccgagc gccgcctgct caacctccat gacgtgattt
      961 tcgaccggtc gaccgaaccg atccccgcaa ggctgatcgg tgttttcgac gacatcgccg
     1021 ccgcgctcgt caagtcgggc gacacctacc gcgagctgat cggcgagatg ctcggcagtg
     1081 ccggttacgg gattcaacgt ggctttgccc tgcacgagac gttcctggaa ctcgtcaagg
     1141 acggcgtcgc ccgcggcgag gtcggcgtcc gtcacgacgc ccagaccctc gccgacatca
     1201 tcgtcggtgc tttgacgggc gggatcgtca attggactgc ggacagcacg tattcgctcg
     1261 agacgggcct ccacgacctg gccgtggcgt tggccgacct gttggtgggc gcggaggatg
     1321 cctgacgatc gtgcgcctgc gtcgcgtcgt ggcatctgcg ccggcgaagg aaaccgttta
     1381 cgacgtctga tcaccgccct gcacggcatc tcggcgcgct gatgtcacca gacctgacag
     1441 tggcgataag cgacggcgtc gccgtgctga cgctgaaccg gcccgaccac ctcaacgcct
     1501 acaccggtga aatggggacg ctgttgggcc aggcctaccg cagctgcgac gaggacgacg
     1561 acgtgcgggc gattgtgctc acaggtgccg ggcaagcgtt ctgcgcgggc gcggacttct
     1621 ccggcggcgc tgatcccttt ggctcacccg gcgacgcctc gtgcttctcc gcgtcgccga
     1681 tcgacccgcc agcgttcgaa ttgcgcacgc cggtcattgc tgcgctcaac ggccacgcca
     1741 ttggaatcgg actgacgctc gcgttgcagg ccgacatccg gatcgtcgcc gcggaggcca
     1801 aatacggtgt cgttcaagtg cgtcggggag tgatcggcga ctgcatgtcg cactggacgc
     1861 tgcccaggct ggccggtgtt gctgtcgcgg cggaaatgct actgacgggc cggaccttcg
     1921 acggcatcga ggcagtggca cggggcattg ccacccgggc ggtgccctcg ggcgaggtgg
     1981 ttgatcacgc gatggagatc gcccgcgata tcgccgtcaa cgtggcaccg ctgtcggcgg
     2041 cactgagtaa gcggctgctg tgggagagcc tgaacaacgg gtactccgca cgcgaagtcg
     2101 cctggatgga gacccagctg catcaccgag tcatgggccg agacgacgcc cgcgaaggtg
     2161 tcgcggcctt tctgggccga cgccccccac ggtggagcgg gtcgctttgt cgtgactggg
     2221 ctgagctgcc gcatccccca gcagtcacgt gacccaggga tgatcgagcg cccgaaagga
     2281 ctgtgggatg cacatcgacc tcctcgccgg gggactcggc ttcaccgaag ggccagtgtg
     2341 gcttgacgat cgcaggatcg cgttgacgtc catcagtcac ggctgcatct acatcgtcga
     2401 cccatccggt ggagcaaccg agcgtatcga gacgggaggc ggacccaacg gtttggcccg
     2461 cggctccgac ggcaccctct ttgtggcgca gaacggggga gtgttcggcg ccagcgggcc
     2521 cgcgcaaccc ggcgtgcagg tgatcacgga tgggcgggtg gagtatctgg tgaccggcct
     2581 tggcgcgccc aacgacttga tgttcgggcc ggacgggcga ctgtgggtca ccgacacccg
     2641 ctgtgagata gacccagtgt cgccagggga tggtctaccg ggacaagtgt gggccgtcga
     2701 tgtcgtcacc ggccacaccg agctgatcgt ggtgagcgga ccggtgttca tcaacgggct
     2761 gggcttcacc cgcgacggac gcacactgct cgtcaccgcc accctcgcag ccgagttgct
     2821 ggcctaccgg cttgcacctg ccgatgccgc ctcgtggcgc caaccgcgtc tggtgcacac
     2881 cttcgacaac ggctggcccg acgggatagc gatcagtgcg cgaggtgaaa cctgggtggc
     2941 attgaccgcc gcggaccgcg tcgacgtcat caacgatgcc ggtgagcgca ccgccagcat
     3001 cacgatgccc ccgggttcgc tgcccaccaa tatctgtatc ggcgtcggac acgccaatga
     3061 gctgttcgtc accgctgcgc acgccgagtc gctgctgcgt atccgcctcg acgaacgcgg
     3121 cgatccgcgc ctttcgccat aaatgcatat cactgcactc ttgcggttgt ctgcatttgc
     3181 tcgtacggtg tggtgatgtc gaaggcgctg tgagcggcga cccgagcgcg gcagaggact
     3241 ggagagcaaa tggccctgac agagcagtca gcaccctcgg taagggactt catggacgac
     3301 cccattgggt ttttcggaga gtcgctgacc cagatgcaca gcatccctcg cgccgagttg
     3361 gagcaactcc agcgaaacgc gatgggtatc cggtttcagc aacaccgcga gagcatcgag
     3421 atgctgcgca agctcgccga ccggctcgac atcaccgaac taagcgatct caacgacgtt
     3481 gtcccgctgt tcttctcgca tactgcgttc aagtcctacc cggcagcgct gatcgacaac
     3541 aagcgcttcg acctgatgac caagtggctg gacaaactca ccagccatga cttgtccgct
     3601 gtcgataccc agggttgcaa cagcatcgac gagtggatcg accggctcga cgaccagacg
     3661 ccgttggaag tgatcacgtc cagcggaacg acgggcacgc tgtctatcct cccgaaggac
     3721 aagcgcggcg cgcgcgaggg catggtgctc tggaagatct gcttattcca gacatttggc
     3781 gtcgaaccca ccgcggagca gctcaatccg gcggtggagg tgatctggcc caactatgcg
     3841 agcggcaagc tgggccacct gcgcatcgcg aacatgctca agggcggttt cacgggcggc
     3901 gacgagagca ggtttcacgc cctgtactcc gattcgatcg acaccgatct gatgttcctg
     3961 gcttccaaga tgcgcgcggc cgcgtctcgg ggagaactgg accgcctggt cgtcgatccg
     4021 gcgctggcag cccgcaaaga cgagttcata gccatgcaga tgcgacagcc ccaagagctc
     4081 gacgtgttct tctccgagat gtccgaacaa ctgcgcggta aaagagtttt catgatcggc
     4141 acctacaacc tgatgtacga cgtcgccaag gcggcccttg agcgcggcat caagggcgta
     4201 ttcgccgagg actccgtgat actcaccggt ggcggctcga agggattcgt tctgcccgat
     4261 ggttacctgg acaccatccg tgagttcctc ggcgtcgacc ggatccagga gggttacggc
     4321 ttctccgaac agagcgcctt ccacttcgcc tgtaccgagg gccgctacca cgttcaacca
     4381 tgggtcatcc cgttcgtcct ggatccagaa accagccggc cgctgccacg cgagggcaga
     4441 cagaccggcc gtgctgcggt ctacgatgtc ctactcaaag cccattgggg cggagtcatt
     4501 tccggcgacg aggtcaccat cgactgggac ttagcatgcc cgtgtggcca gacgagcgtg
     4561 gctttcgagc acgacatcat ccgctacagc gagaaacgag gtgttgagga cgatcgcatc
     4621 acctgcgctg cgacgcacga agtccacaac gaggccgtga acttcatgaa ggagttcgag
     4681 tcgtgagcgc ggcattcacc gttccgctgt tcctgcgcgg ccagatcatc accgacgacc
     4741 tggtgtcttt cggcatccgt ggcggcgggg cggagtttca gagccccgac atgagtaagt
     4801 acgtcgaccg cctgccgctg gccagcccga tggagatggc ggacctctac gacatcggct
     4861 tcgatgagat cctcgacgtg ctggaggccg tcggcgatgc gctggcgttc gaaaggaacg
     4921 cccacctgca ggaggcgtat gaggctgcga tactggccaa tccgctgccg gcggtgatgc
     4981 tgatgaacag ctataagatt ctgcagccgc ttttcgcgag ggccaacgtg ctcgaggtcg
     5041 ccgacagtca ggtcggtctc gactatctca acgggtgggt tccgcagcgc ctcgccgacg
     5101 gtcgtgaact gcgggtgcga gcattcggct cacgggtgct gcacattccc gccggaaacg
     5161 gcgggctcgt ctcggcggtc accattctgc ggtcggtcat cgcccgttgc gacaccatca
     5221 tcaaggcgcc ctcgaacgac ccgctgaccg caatggccat cgcacgaacc gtggccgacg
     5281 tcgcgcccga tcaccccatc accaaacacc tcgcggtcgg ctactggaag ggcggtgatc
     5341 t
//
LOCUS       DASYLX010000154         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2092113,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000154 DASYLX010000000
VERSION     DASYLX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092113"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VGP27_03725"
     CDS             complement(<1..140)
                     /locus_tag="VGP27_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV7850460.1"
                     /translation="MPYPSHRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEP
                     KP"
     gene            complement(175..1818)
                     /locus_tag="VGP27_03730"
     CDS             complement(175..1818)
                     /locus_tag="VGP27_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional uroporphyrinogen-III
                     C-methyltransferase/uroporphyrinogen-III synthase"
                     /protein_id="HEV7850461.1"
                     /translation="MTTRGRKQRPGRITFVGSGPGDPGLLTTRARTVLANAALIFTDP
                     DVPEAVLDLCGSELPPPSGPKQAEPATDADGDGETVTAIPAGPDIRPALGDPAEVAKT
                     LATESRTGVDVVRLVAGDPLSVDSVITEVNVLSRSHLTFEIVPGLPATTAVPSYAGLP
                     LGSTHTEADVRGDVDWAALAAAPGPLILQATASHLADAAGTLIEYGLADTTPCVVTAQ
                     GTTCQQRSVETTLGGLNDKAAIGANEPAGPLTGPLVVTIGKTVANRGKLNWWESRALY
                     GWTVLVPRTKDQAGEMSEKLVSHGALPIEVPTIAVEPPRSPAQMERAVKGLVDGRYQW
                     VVFTSTNAVRAVWEKFKEFGLDARAFSGVKIACVGHSTAERVRAFGIEPELVPSGEQS
                     SLGLLDEFPPYDEIFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPP
                     PADTREMIKTGGFDAVCFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAAEFGLRV
                     DVQPEVAAVGPLVEALAEHAARLRAEGALPPPRKKSRRR"
BASE COUNT          278 a          683 c          612 g          285 t
ORIGIN      
        1 atcggtttcg gctcgtcgat gccgtcggcg acgaacatcg gcagcaccag atgccttggc
       61 tccaaagacg tctgcgcgac cagtcgacga agcgccggtg tcgaccggag tcgacgcgga
      121 cggtgactcg gatagggcat gtaaagccat ccctccgtga ctacgccgac gggcttagcg
      181 gcggcgactc ttcttgcgcg gcggcggcag tgcgccctcg gcccgcagcc gggcggcgtg
      241 ctcggccagc gcctccacca acggacccac cgcggccacc tccggctgca catcgacgcg
      301 cagcccgaac tccgctgccg tttccgccgt cttgggcccg atgcacgcga cgatcgtgcg
      361 ggcgtgcggc ttgcccgcga taccgaccag gttgcgcacc gtcgagctcg acgtgaagca
      421 gaccgcgtcg aagccacccg tcttgatcat ctcgcgggtg tccgcaggcg gcggtgcggc
      481 acgcaccgtg cggtaggcgg tgacgtcctc gatctcccag ccgcgctcac gcaagccttc
      541 ggccagcgtc tcggtggcga tgtcggcccg cggcagcagc accctgttca ccgggtcgaa
      601 aatttcgtcg tacggcggga attcgtcgag cagccccagc gaggactgct cacccgacgg
      661 caccagttcc ggctcgatac cgaacgcgcg cacgcgctcg gcggtggaat gccccacaca
      721 cgcgatcttc acaccggaga aggcgcgagc gtcgaggccg aactctttga acttctccca
      781 caccgcgcgc accgcgttgg tcgaggtgaa caccacccac tggtagcggc cgtcgaccag
      841 acccttgacg gcgcgctcca tctgcgccgg gctgcgcggc ggctccaccg cgatcgtcgg
      901 cacctcgatc ggcaatgcgc cgtgcgagac cagcttctcg ctcatctcgc ccgcctggtc
      961 cttggtgcgc ggcaccagca cggtccagcc gtacagcgcg cggctctccc accagttgag
     1021 ctttccgcgg ttggccaccg tcttgccgat cgtcacgacc agcgggccgg tcagcgggcc
     1081 cgcgggctcg ttcgcaccga tcgccgcctt gtcgttgaga ccgccgagcg tggtctccac
     1141 cgagcgctgc tggcacgtgg tgccctgcgc ggtgacgaca cacggcgtgg tgtcggccaa
     1201 tccgtattcg atcagcgtgc cggccgcgtc ggccagatgt gacgcggtgg cctgcaggat
     1261 cagcggcccc ggcgcggcgg ccagcgcagc ccagtccacg tcgccgcgaa cgtcggcttc
     1321 ggtgtgcgtc gaccccagcg gaaggcccgc ataggacggc acggccgtcg tggcgggcag
     1381 gccgggcacg atctcgaacg tcaggtgcga gcgcgacagc acgttgactt ccgtgatcac
     1441 cgagtccacc gaaagcggat ctcccgcgac cagccgaacc acgtcgactc cggtcctgga
     1501 ctcggtcgcc agcgtcttgg ccacctcggc cggatcacca agtgcgggcc ggatatccgg
     1561 accggcagga atcgcggtca cggtctcgcc atcaccgtcg gcgtccgtcg cgggctcggc
     1621 ctgcttcggg cccgacggcg gcggcagttc ggacccacac aggtccagca ccgcctcggg
     1681 cacatcgggg tcggtgaaaa tcagtgcggc attggccagc acggttcgcg cacgcgtcgt
     1741 cagcaggccc gggtcgccgg gcccggagcc gacgaacgtg atgcggccgg gcctctgctt
     1801 acgccctcgg gtcgtcatct gtctctccca gcttcgacca acagatcgcg tgccccca
//
LOCUS       DASYLX010000155         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2530120,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000155 DASYLX010000000
VERSION     DASYLX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530120"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1667)
                     /locus_tag="VGP27_03735"
     CDS             complement(321..1667)
                     /locus_tag="VGP27_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7850462.1"
                     /translation="MALGPWRHDVGRWSLMHGWLPVTVQAFSAAMLAVGLSRRSRRWL
                     MLWLPVTVTAGLAMALWARWYIRSVGVAGDPAPASLWIWIGLSGSTMALMVSGWRGVG
                     WMRRGVSVMAVPLCLLAAALALNQWVGYFPTVRALWTQISAGPLPNQTDRATVTAMQL
                     RGAKPPKGVVVPVNIGAQASGFKHRGELVYLPPAWFASNPPPPLPVVMMIGGEFNTPS
                     DWVRAGDAVSAADGFAAAHGGDSPVLVFVDSGGAFNIDTECVNGPRGNAADHLTKDIV
                     PFMISNFGVSRQRARWGVAGFSAGGTCAVDLAVMHPELFSAFADLAGDLSPNSGTKAQ
                     TIDRLFGGSVEAWSRFDPSTVITKHGPYQGVSGVFTVVSGQDTAAKTLCGLALTNGIE
                     CTVATVPGEHDWPFAGRAWAAALPWLAGELGTPGTLKPQLSPQRSGTSATAPSNTR"
     gene            complement(2219..2614)
                     /locus_tag="VGP27_03740"
     CDS             complement(2219..2614)
                     /locus_tag="VGP27_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV7850463.1"
                     /translation="MTRHALSAAAIAAAVAALGITVAATADAKTSPNVVGQKYGDASS
                     ALSSAGFTPVVSTTVGDRLAWSNCIVFHQRDRSMPAPPNSGGSATNQTLVSLNCDQAV
                     ASAIVPGNSLGSPEGRSAAAAAAAAKAKQ"
     gene            complement(2641..2931)
                     /locus_tag="VGP27_03745"
     CDS             complement(2641..2931)
                     /locus_tag="VGP27_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850464.1"
                     /translation="MLAKQLKTTLCARPKPLGILVGAAALTTLGALSFCATPPAATTV
                     EIPLAGSGSAPMNTTFTQPVVAGMTLGNTATATTAASMPAVSMAVPAVKAGH"
     gene            complement(3088..3425)
                     /locus_tag="VGP27_03750"
                     /pseudo
     CDS             complement(3088..3425)
                     /locus_tag="VGP27_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891919.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
     gene            3638..4327
                     /locus_tag="VGP27_03755"
     CDS             3638..4327
                     /locus_tag="VGP27_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850465.1"
                     /translation="MKVLLVEDELHVAASLKVGLRAEGFVVVHADTGTEGFWQAVENR
                     FDVIILDIMLPGPSGYEVLRRIRERKIWTPVLMLTAKDGEYDQADAFDLGADDYLVKP
                     FSFVVLVARLRALIRRGAPQRPVVMTAGDLVLDPSRHLVTRAGVPIDLSPREFGVLEF
                     LMRNTDVVCTKAQIVQNVWDEHFSGPENVVEVYIGYLRRKIDVPFGTNTIETVWGVGY
                     RLESGNLASGN"
     gene            complement(4288..>4988)
                     /locus_tag="VGP27_03760"
     CDS             complement(4288..>4988)
                     /locus_tag="VGP27_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7850466.1"
                     /translation="LARIESGHAAQRQFVGDASHELRSPLTAIISALDVAVVHPEIFD
                     LQQAASTLVPEAERMRVLVEDLLLLARADEGRLAMHREPVRLDDIVNGEVVRQRCIGQ
                     CDIDVCAEPTELSADSAAIYRLVRNLLENAVRHAHSRVVCTVRPSGEYAILTVADDGP
                     GIPQADRVRVFGRFVRLETDRARTSGGTGLGLAIVAEIATAHGGIVTLDEHPGGGALV
                     RVQLPLAKLPDSKR"
BASE COUNT          916 a         1607 c         1564 g          901 t
ORIGIN      
        1 accgggcaga cggtcaccac aacgactcct ccatccgttc cggaggtaag cgtggcgacg
       61 ccgagcgtga ctgcgacgac gccatcgggc ttcgcgccgg cccccggtcc gtaggtgggg
      121 tgtctaaccc cagccgcgat acattcgcgc ggcgccatgc gcgacgctag cgactactgg
      181 cagcggtgat tggtcgtgcc ggttcgtccc cggccagagg catcgcggcg cgcacgaatt
      241 cctgcgactg gcgttcgcca atcaggaact gatcccctgc gatcgccgtt gaaaagccgc
      301 ggcgtcattc ggccgctcat tcatcgagtg ttcgagggtg ccgttgctga cgtaccgctc
      361 ctctgaggcg ataactgcgg cttgagcgtc ccaggagtgc cgagttcccc ggccaaccag
      421 ggcagcgcag cagcccacgc tctaccagcg aaaggccaat cgtgctcgcc gggcacggtc
      481 gcgacggtgc actcgatgcc gttggttagc gcgagtccgc acagcgtctt ggcggcagta
      541 tcttgccctg agacaacggt gaacactccg gaaactcctt gataggggcc atgtttagta
      601 atgaccgtgg agggatcgaa ccgggaccac gcctccacgc tgccgccgaa cagcctgtcg
      661 atggtctgcg ccttggtacc cgagttcggg ctcaaatcac cggcgaggtc tgcgaaggcg
      721 ctaaacagct cgggatgcat gaccgccaga tccacggcac aggttccgcc agccgagaag
      781 ccggcgacac cccaccgagc ccgttgccta ctcactccga agttggagat catgaacggg
      841 acgatgtcct tcgtgagatg gtcggccgca ttgccgcggg gaccgttcac gcattcggtg
      901 tcgatgttga aggcgccgcc ggagtcgacg aacacgagca caggagaatc accgccgtgc
      961 gcggcggcga atccgtctgc tgcgctgacc gcatcgccgg cgcgcaccca atcagacggg
     1021 gtgttgaact caccgccgat catcatcacc acgggcaacg gcggtggcgg attgctggcg
     1081 aaccatgccg gcggcagata cacgagttct ccgcgatgtt tgaatccaga cgcctgagcg
     1141 ccgatgttga ccggcaccac cacccccttg ggtggcttgg cccccctcaa ttgcatggcg
     1201 gtgacggtcg cccgatcggt ttgattgggc aacggacccg cgctgatctg tgtccagagg
     1261 gcgcgcaccg tcgggaagta cccaacccac tggttgagcg ccagcgctgc ggcgagcaga
     1321 cacagcggca ctgccatcac tgatacgccg cgccgcatcc atcccacgcc tcgccacccc
     1381 gacaccatga gcgccatggt tgagccagac agcccgatcc agatccacag cgacgctggc
     1441 gctggatccc cggccacccc caccgaccgg atataccagc gcgcccacaa agccatcgcc
     1501 aacccagcgg tcaccgtgac cggcagccac agcatcagcc agcgccgcga tcggcgactg
     1561 agcccgactg ccagcatcgc tgcactgaag gcctgcaccg tcaccggcag ccagccatgc
     1621 atcaacgacc atcgccccac gtcgtgacgc catggcccca aagccatagc gacgcttgcg
     1681 gtcgtcgctg cagttaacac ggcccctgtg atgagcgccc gccgtcgacg gcggctccac
     1741 gacatcggtt ggacggtgtt cgccacggtc gcggtcgtcg ccggcggcgg caccactgcc
     1801 gtacgacccg tcacctcgat ccccttaagt gtccgccgct gcgcccacaa agtaatttta
     1861 tggtgcccta cacctactga gtatccgctg aaaaaacctg acatcggtaa gcgggctgtt
     1921 gggcctacgg cgcccctcgt gcggcgccga cgaggatgcg cagcgtaccg ggcgcctgga
     1981 ttcgtagctc gtgccgaata caccaattcc cctgcgcttt gctgtttctg gccgcttcac
     2041 ccgtacgccg tgaacctcga tcggtgtggc ggattcgacc agcagctggg cgcaaaccct
     2101 tcgcttgcac atacggtgtc gagggtcctc gagtcgcgaa ccggggcgtc cgcagatcag
     2161 aggcggctat cgacacttgg ccggctacca ggatcctgtg gcgcgattgt ttcgccgatt
     2221 attgcttggc tttggcagcc gcagcggccg ccgccgcgga ccggccctcg ggactgccca
     2281 gcgagttgcc cgggacaatg gccgacgcca ccgcttggtc gcagttgagg gacaccagcg
     2341 tctggttcgt cgccgagccg ccgctattgg gtggggcagg catggatctg tcccgctggt
     2401 ggaacacgat gcagttcgac caagccaacc ggtcaccgac ggtggtcgac actaccggtg
     2461 tgaatccggc tgagcttagt gcggaactgg cgtcgccgta cttctggccc accacgttgg
     2521 gcgaggtctt agcgtcggct gttgcagcga cggtgatgcc cagggcagcg acagcagcgg
     2581 cgatggctgc ggccgataaa gcgtgtctgg tcatgaaggt tcctagtacg ggttaagggg
     2641 tcagtgaccg gctttgaccg cgggcactgc catggagacc gccggcatcg aggccgctgt
     2701 cgtcgcagtg gcggtgttgc ctagtgtcat gccggcgacg acgggttgag tgaacgtggt
     2761 gttcatcggg gcgctacccg acccggccag tggtatttcg acggtcgtgg cggccggtgg
     2821 ggtcgcgcag aagctcaagg ctcctagcgt ggtcagtgct gcagcgccga caagaatgcc
     2881 cagcggcttg gggcgtgcgc acaaggtggt tttcagctgc ttagcgagca tgacgccagt
     2941 ctcgcgccga agcgttgata ggacgctgag tagagtctgg gacttgaacg ccggttcctg
     3001 acgaaacgct gggagctgaa caattcgact taccgtagcg tcttcaggct caccgcccgc
     3061 gtccgttccg gctaggtacg cgggacgtta acttggcgca agctcattca tgttctgcgg
     3121 ctcgccgtat gtggtgacgc tgtgattcga tttggaaatc aggtgcggca aacggtcgaa
     3181 gcaagagctc gccggccgcg ccggtcacgg tgccgtgcgc gttggagacc gcaaccttgc
     3241 cgtgagggcc atcgacgtca agacaacgtc gccacttttc gaatgccccg accgttgccc
     3301 aaatcggcgc taaccgacac gaaggaacac cagttcggcg tggtgtagct gaagttatgc
     3361 ccactcctag agaggggcga tccaatccga tgaccgagct cgagcgtccc ttcgaacttg
     3421 tcggcagcct tacccccagg ttgtacacgg cgctgccgga cgcacacaag aactgcggcg
     3481 tgacggcgca cgagtcactc agcgtttcct cagtgctgct ctgcgatggt gacccaggtg
     3541 ctacaagcgc cgtcggtttt cagaaattgc cggtgcgccg atgcagccaa ttaccggcgt
     3601 agcgactgta gcccgccacg acaggggagt tcgtccggtg aaagtcctgc tggtggaaga
     3661 cgagctacat gttgcggcca gtctcaaagt cggtctgcgc gcagaaggct tcgtcgtcgt
     3721 gcacgccgac accgggacag agggattttg gcaggccgtc gaaaatcggt tcgacgtcat
     3781 cattttggac ataatgctgc ccggtccgag cggctacgag gttctgcggc gaattcgcga
     3841 gcgcaagatc tggacaccag tgctgatgct caccgccaag gacggtgagt acgaccaagc
     3901 cgacgcgttc gatcttggcg ccgacgatta cctggtcaag cccttctcgt tcgtggtgct
     3961 cgtcgctcgg ctgcgggcat tgatccgtcg cggtgcaccg cagcgtcccg tcgtgatgac
     4021 cgccggcgat ctcgttctcg atccgagccg gcacctggtg acccgcgcgg gtgtgcccat
     4081 tgatctcagt ccccgcgagt tcggcgtgct ggaattcctg atgcgcaaca ccgacgtggt
     4141 gtgtacgaag gcccagatcg tgcagaacgt gtgggacgag cacttctccg gtcccgaaaa
     4201 cgtcgttgag gtctatatcg gttatctgcg acgcaagatc gatgtgccct tcggtacaaa
     4261 cacaatcgag acggtttggg gagtcggcta ccgtttggaa tcgggcaact tggctagtgg
     4321 caactgaacc ctgaccaatg caccccctcc ggggtgctca tcgagggtga caatgccacc
     4381 gtgcgcagta gcgatttcgg caacgatggc caagcccagc ccagtgccac cgctggtgcg
     4441 cgcacggtcg gtctccaacc ggacgaaccg gccgaagact cgcactcgat cggcttgcgg
     4501 gataccgggg ccatcgtcgg cgacagtcaa gatcgcgtac tccccagaag ggcgtacggt
     4561 gcacacgaca cgtgaatggg catgccgcac agcgttttcc agcaagttgc gcaccagccg
     4621 gtaaattgcc gcgctgtccg cgctcagctc ggtcggctcg gcacagacat cgatgtcgca
     4681 ctgtccgatg caacgctggc gcaccacttc gccattcacg atgtcatcaa gccggacagg
     4741 ctcgcggtgc atcgccagtc ggccctcatc agcgcgagcc agtaacagca ggtcctccac
     4801 gaggacccgc attctttccg cttcaggcac aagggtgctc gctgcctgct ggagatcgaa
     4861 gatctccggg tgaaccaccg cgacgtcaag tgccgagatg atcgccgtca acgggcttcg
     4921 tagctcatgg gacgcatctc cgacgaactg gcgctgtgcc gcgtggccgc tttctatacg
     4981 agccaaca
//
LOCUS       DASYLX010000156         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_7313, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000156 DASYLX010000000
VERSION     DASYLX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7313"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGP27_03765"
     CDS             complement(<1..399)
                     /locus_tag="VGP27_03765"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEV7850467.1"
                     /translation="MVALIVHAILGIAVIAFIVRSNPAVFSRVPTGPQLSPMEIFLWF
                     LGIISLPISWYFNIQYVQQYAENPFWGQPTWAEFIAMGYANPSSSSQVVDYTIMSVLL
                     AVWVVVDGRRRGFKHAWLYFGLFLFTSSTFG"
     gene            469..1074
                     /locus_tag="VGP27_03770"
     CDS             469..1074
                     /locus_tag="VGP27_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850468.1"
                     /translation="MVRPAQTARSERTREALRQAAVVRFLAQGVENTSAEQIAADAGV
                     SLRTFYRHFASKHDLLFADYDAGLHWFRSALATRSPDESIIESVQSAILARPYDDWAV
                     AKIPTLRAQELDPGRILRHIRQVETEFAAVIEENLGGEDPPMPGSDDRMRITVISRCI
                     AAAVFGAMEVWMLGEDRSLPELTRLTREALQTLDKGIRDAV"
     gene            1126..>2294
                     /locus_tag="VGP27_03775"
     CDS             1126..>2294
                     /locus_tag="VGP27_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850469.1"
                     /translation="MADYDAIVVGAGHNGLTAAALLQKAGRRTLCLDFKLYAGGMAST
                     VELFDGFKFEIAGSVQIPTAAVVSRELGLDTLPTVDLDVMSVSLRGVGDEPLIYYTDP
                     MKLLTHINEVHGAEAVNGMAGLMAWCQAPTRALGRFDAGRPPKTLDEMYACATNEFER
                     STITDLLFGSVTDVIDRYLPDKDKNGALRGMLSLLACNTTYRGPATPGTAAALAYGFA
                     VPDENALLVKKLKGGIGSLTAHLHDLFVSNGGELRLRSEVDEILVSDGRVAGVRLQDG
                     STISAPVVISGVAPDLTINGLVESSAVPADIRERFSRSDHRGSYLQMHFALDGIPEFA
                     APYEVLNHPQMQSNIGIFSTPEELQQQWEDCRRGIVPADPSIALQIPSVNDPDLAP"
BASE COUNT          430 a          729 c          739 g          396 t
ORIGIN      
        1 gccgaacgtc gaactcgtga acaggaacaa cccgaaatag agccatgcgt gcttgaaccc
       61 gcgcctgcga ccgtcgacga cgacccacac cgccagcagc acgctcatga tggtgtagtc
      121 gacgacctgt gaactcgacg aagggttcgc gtagcccatc gcgatgaact ctgcccaggt
      181 gggctggccc cagaacgggt tctcggcgta ttgctgcacg tattggatgt tgaagtacca
      241 gctgatcggc aaggagatga tgccaaggaa ccacaggaag atctccatcg gggagagctg
      301 cggtccggtt gggacgcggc tgaagacggc cggattcgat ctgacgatga atgcgatgac
      361 cgcgatgccg aggatcgcgt gcacgatgag ggcaaccatt tttggagtct ctcgaccgat
      421 ccaagttttg tcaatattga cataaccttc atagggtaat ttacgcccat ggtccgacct
      481 gcccagacgg cccgcagcga gcgcacgcgc gaggcgctgc ggcaggctgc ggtggtccgg
      541 ttcctcgccc agggcgtcga gaacacgtcg gccgaacaga tcgcggccga cgccggggtg
      601 tcgctgcgga ccttctaccg ccacttcgcg tcgaagcacg atttgttgtt cgccgactac
      661 gacgccggtc tgcactggtt ccggtcggcg cttgcgacgc ggtcgcctga cgagtcgatc
      721 atcgaatccg ttcagtcggc gattctggcc agaccgtacg acgactgggc ggtcgccaaa
      781 ataccgacgt tgcgggccca ggaactcgac cccggccgta tcctccgcca catccgtcaa
      841 gtcgagacgg agttcgccgc agtcatcgag gagaacctcg gcggcgagga cccgcccatg
      901 cccggatccg acgaccggat gcggatcacc gtcatctcgc ggtgcatcgc ggccgcggtg
      961 ttcggtgcga tggaggtctg gatgctgggg gaggaccgct cgctgccgga gttgacgcgg
     1021 ttgacccggg aagcgctgca gacgctggac aaggggatca gggacgcggt ctagccaagt
     1081 ttcgtcaata ttgacaaaac tttgcgctcg tgtcagcctc agccaatggc ggactatgac
     1141 gcaatcgtcg tcggcgcggg tcacaacgga ttgaccgcgg cggcactgct gcagaaggcc
     1201 gggcggcgca ccctgtgcct cgacttcaag ctgtatgccg gcggaatggc ttccaccgta
     1261 gagcttttcg acggcttcaa gttcgagatc gcaggctcgg tgcagattcc gacagcggcc
     1321 gttgtcagcc gcgaactggg cctggacaca ctcccgacgg tcgacctcga cgtgatgtcg
     1381 gtgtcgctgc ggggagtcgg cgacgaaccg ctgatctact acaccgaccc gatgaagcta
     1441 ctgacgcaca tcaacgaggt tcacggcgcc gaggccgtca acgggatggc ggggctgatg
     1501 gcgtggtgtc aggcgccgac acgggcgttg gggcggttcg acgcaggccg gccgccgaag
     1561 acgcttgacg agatgtatgc ctgcgccaca aacgaattcg agcgatcgac gatcaccgac
     1621 ctgttgttcg gttcggtcac cgacgtgatc gaccgctacc tgcccgacaa ggacaagaac
     1681 ggtgcgctgc gcggcatgct ttcgctgctc gcgtgcaaca ccacctatcg cggaccggcg
     1741 accccgggca ccgccgcggc gctggcgtac ggcttcgccg tgccggacga gaacgcgctg
     1801 ctggtcaaga aactgaaggg cggcatcggt tcgctcaccg cgcaccttca tgacctgttc
     1861 gtctcgaacg gcggcgaact gcggttgcgc agcgaggtcg acgagatcct ggtctccgac
     1921 ggccgcgtag cgggcgtccg cctccaagac ggctcgacga tctcggcgcc ggtcgtcatc
     1981 tccggtgtcg cgcccgacct gacgatcaac ggcctggtcg agtccagcgc ggtgcccgca
     2041 gacatccggg agcgcttttc gcgatccgac caccgcggca gctatctgca gatgcacttc
     2101 gcattggacg gcatcccgga gttcgcggcg ccctacgagg tgctcaacca tccgcaaatg
     2161 caatccaata tcggcatctt cagcacaccc gaagagctgc agcagcagtg ggaggattgc
     2221 aggcgcggca tcgtgcctgc cgatccctcg atcgccctgc agatcccctc cgtcaacgac
     2281 ccggacctgg cgcc
//
LOCUS       DASYLX010000157         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_117357,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000157 DASYLX010000000
VERSION     DASYLX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117357"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGP27_03780"
     CDS             <1..418
                     /locus_tag="VGP27_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV7850470.1"
                     /translation="GAYQTMFARGEVRKTYLARAAVDPSLAFPLTVRSRIIKERGRLQ
                     AFEEPGEPNAETRIEHLGDGLYRLTPRTGRTHQLRVHMASLGIPITGDPFYPNVVDVA
                     RDDFSTPMRLLAHSVEFADPLTGCLRRFQSGRTIDE"
     gene            415..1380
                     /locus_tag="VGP27_03785"
     CDS             415..1380
                     /locus_tag="VGP27_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEV7850471.1"
                     /translation="MIGVSVGRLWQYPVKSMQGEEVDQIVLGPGGVAGDRAFGLVDVE
                     TGRLASAKRPRRFGALLECRARFLTQPTDASEPPPVEVTFPDGTVVCTADDSGADLTR
                     HATDLLGREVRLISTAPEGIALDELIPQIEGLGENALDAMQTEPDSYGDSFIPIPAAM
                     AAPGTMLDLAAMHVLATSTLQRMADAHPDGRWDPRRMRPNILIDDSAVPGDEDDWLGC
                     DLHIGEQAVVHIVGPTPRCVMTTLPQPGLQKDSNILKTVAKVNMKDLGPLGRMPCAGS
                     YAEVVTPGVVRRGDSIRFERVEPRKGALAATFEMMAAVLAAGNGQ"
     gene            1377..>1749
                     /locus_tag="VGP27_03790"
     CDS             1377..>1749
                     /locus_tag="VGP27_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEV7850472.1"
                     /translation="MSPPLEPTPEQMAALAARPAAEPVVMVNLLQFTPDGGRESYARY
                     AQEVAPHLRRVGGTVRYAGGAPMQVIGDDEKPWWDAILVVEYPSPAAFIDMVTNAEYL
                     EIHHYRANALERGDLIATTWTM"
BASE COUNT          305 a          567 c          590 g          287 t
ORIGIN      
        1 gggcgcgtat cagacgatgt tcgcgcgcgg cgaggtgcgc aagacgtatc tggcgcgcgc
       61 tgcggtcgac ccctcgctgg cgttcccgtt gacggttcgg agccggatca tcaaggagcg
      121 cgggcgttta caggcgttcg aggagccggg cgagccgaac gccgagacgc gcatagaaca
      181 cctcggcgac gggttgtacc ggttgacgcc gcgtaccggc cggacgcatc aactgcgggt
      241 gcacatggcg tcattgggca ttccgatcac tggagacccg ttttacccga acgtcgtgga
      301 cgtggcccgc gacgacttct cgacgccaat gcgtctgctg gcccacagcg tcgagttcgc
      361 ggatccgctg accggatgtt tgcgtcggtt ccagagcggg cgcactatcg acgaatgatt
      421 ggtgtatcgg ttggcaggct gtggcagtac ccggtcaagt cgatgcaggg cgaagaggtc
      481 gaccaaatcg tgctcggccc cggcggagtg gccggcgacc gcgcgttcgg gctcgtcgac
      541 gtcgagaccg gtcggcttgc cagcgccaag cgacctcggc ggttcggcgc gcttctcgag
      601 tgccgtgcgc gattcctcac gcagcccacc gacgcatccg aaccgccgcc cgtcgaggtc
      661 acttttcccg acggcactgt ggtgtgcacc gccgacgaca gcggcgccga tctgacacga
      721 cacgcgaccg atctgcttgg tcgcgaggtc cgcctgatct ccacggcccc ggaaggcatc
      781 gcgttggacg agctgatacc gcagatcgag ggactcggcg agaacgctct ggacgccatg
      841 cagaccgaac ccgacagcta cggcgacagc ttcataccga ttcccgccgc gatggccgcg
      901 cccggcacga tgctggatct ggcggccatg cacgtgctgg ccaccagcac cttgcagcgc
      961 atggccgacg cccatcccga cggccgctgg gatccaagac gcatgcggcc caacattttg
     1021 atcgacgaca gcgccgtgcc gggcgatgaa gacgattggc tcggatgcga tctgcacatc
     1081 ggcgagcagg cggttgtgca catcgtcggg ccaacgccgc gctgcgtgat gacaacgctg
     1141 ccgcagcccg gcctgcaaaa ggactccaac atcctcaaga cggtcgcgaa agtcaacatg
     1201 aaggatttgg gtcccctggg tcgaatgcct tgtgccggtt cgtatgccga ggtcgtgacg
     1261 ccgggcgtgg tgcgacgcgg cgattcgatt cgctttgaac gcgtcgagcc tcgcaaaggt
     1321 gcgctggccg ccacgttcga gatgatggcc gctgtgctcg cggcagggaa cggccaatga
     1381 gcccgccact ggagccgacg cctgagcaga tggcggcgct tgcagcgcga cccgcagccg
     1441 aaccggtcgt catggtgaac ctgctgcagt tcacacccga cggcggtcgc gaaagctatg
     1501 cccgctacgc acaggaggtc gctcctcacc tgcgacgggt cggcggcacc gtccgctacg
     1561 caggcggcgc gccgatgcag gtcatcggcg acgacgagaa gccgtggtgg gatgcgattc
     1621 tggtcgtcga gtacccgtcg cccgcggcgt tcatcgacat ggtgactaat gcggagtatc
     1681 tcgagatcca ccactatcgc gcgaacgcac tcgagcgcgg cgatctgatc gccacgacgt
     1741 ggacgatgg
//
LOCUS       DASYLX010000158         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_226650,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000158 DASYLX010000000
VERSION     DASYLX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226650"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..362
                     /locus_tag="VGP27_03795"
     CDS             102..362
                     /locus_tag="VGP27_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850473.1"
                     /translation="MTSMNGPSGRDSANGKHARNDAAPDQPPRAQFLTVAEVASLMRV
                     SKMTVYRLVHNGELPAVRVGRSFRVHAKAVHDLLETSYFDAG"
     gene            558..659
                     /locus_tag="VGP27_03800"
     CDS             558..659
                     /locus_tag="VGP27_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEV7850474.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRRTRVQRRKLGK"
     gene            689..1786
                     /locus_tag="VGP27_03805"
     CDS             689..1786
                     /locus_tag="VGP27_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850475.1"
                     /translation="MDSKDRSGRRSQGRDDDTLHQPKVVLVTGACRFLGGYLIARLAQ
                     NPSIDHVIAVDAVAPSKDLLRRMGRAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASY
                     APRSGGKAALKEFNVMGAIQLFAACQKAPSVRRVVVKSTSEVYGSSAFDPVLFTEDSS
                     SRRPPGDGFARDSIDIEGYARGLARRRPDISLTILRLANMIGPAMDTALSRYLAGPVV
                     PTVVGHDARLQLLHEQDALGVLERATVSGRSGTFNVGASGIIMMSQAIRRAGRVALPV
                     PRFGLWAVDSLRRATRYTELDREQLDYLSYGRVMDTARMRNELGYNPKWTTTEAFDDY
                     VRGRGLTPIVDPRWVRSLESRAVSAAQRWGR"
     gene            1818..>2880
                     /locus_tag="VGP27_03810"
     CDS             1818..>2880
                     /locus_tag="VGP27_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7850476.1"
                     /translation="MDVAGESKAKVIPLHSNTGRQAAQRRAAARGEGARRHPSLLSDP
                     NSRASADQIAAVVREIDERRSAAAGTPPADESPNELAQRIASVAEFIRKRMTGDYTVD
                     EFGFDRHLNNSVFLPLLRVLFNSWFRVEVSGIENLPETGAALVVANHAGVLPMDGLMV
                     SVAVHDKHPAQRDLRLLAADMVFTMPVMGQAARKAGHTMACTSDAHRLLDAGELTAVF
                     PEGYKGLGKHFNDRYKLQRFGRGGFVSAALRAKAPIVPCSIVGSEEIYPMIADVKLLA
                     RLFGLPYFPITPLFPLAGPAGLLPLPSKWHIEFGEAIETADYDDAAADDPMITFELTD
                     QVRETIQQTLYQLLANRRNM"
BASE COUNT          504 a          899 c          981 g          496 t
ORIGIN      
        1 ataaccccaa taaccccgct attctcctcg tgtaagcgcg gggtcgtacc cagcggtggg
       61 gaagccgctg gaacagcccg tgcctgaagg attgggttgc gatgacgtct atgaacgggc
      121 catcggggcg ggattcggcg aacgggaagc atgcgcgtaa cgatgcggct cccgaccagc
      181 ccccgcgggc tcaatttctc accgtcgccg aagtggccag cctcatgcgg gtcagcaaga
      241 tgacggtcta ccgcctggtg cacaacggcg agctacccgc ggtgcgggtc ggcaggtcgt
      301 tccgcgtgca cgccaaggcg gtccacgact tgctggagac gtcgtatttc gacgccgggt
      361 gacgatcaag cggcgtagcc gcgttgagga agccggtgcg acagggcagc cgggtgacga
      421 tcaagcggcg tagccgctcc tcgcgcgagc gctcgtcagt cgtcggtttt ttctccgcac
      481 cggtggcccg gtaaagtatg cgggtcgagc caaatggcgt gagaagacgt cttgaaacaa
      541 tctaggtagc ggagttcatg ggttcagtca tcaagaagcg gcgtaagcgc atgtccaaga
      601 agaagcaccg caagctgctt cgtcgcaccc gggtgcagcg cagaaaactc ggcaagtagg
      661 actactgctc cgtcgatagg ctgggcggat ggattccaaa gatcgctccg ggcgccgcag
      721 ccagggacgc gacgatgaca ccctgcacca gcccaaagtg gtgctggtca ccggcgcctg
      781 tcggttcctc ggcggctatc tgatcgcccg gctcgcgcag aatccgtcga tcgaccacgt
      841 catcgccgtc gacgcggtcg cgccgagcaa ggatctgctg cggcgcatgg gccgcgcgga
      901 gttcgtccgg gccgacatcc gcaacccgtt catcgccaag gtcatccgaa acggtgacgt
      961 cgacaccgtc gtgcacgccg cggcggcgtc ctacgcgccg cgctccggcg gcaaggcggc
     1021 gttgaaggaa ttcaacgtga tgggcgcgat ccagctgttc gcggcgtgcc agaaggcgcc
     1081 gtcggtgcgt cgcgtcgtcg tcaagtcgac gtccgaggtg tacgggtcga gcgcattcga
     1141 cccggtgttg ttcaccgagg actccagcag caggcggcca cctggcgacg gcttcgcccg
     1201 cgacagcatc gacatcgagg gctatgcgcg cgggctcgcg cggcgcaggc ccgacatctc
     1261 gctgacgatc ctgcggttgg ccaacatgat cggcccggcg atggacaccg cgctgtcgcg
     1321 ctatctggcg ggtcctgtgg tgccgaccgt cgtcgggcac gacgcgcggc tgcagctgct
     1381 gcatgagcag gacgcgttgg gcgtcctcga gcgcgcgacc gtgtcgggca ggtcaggcac
     1441 cttcaacgtg ggcgcctccg gaatcatcat gatgtctcag gccatccggc gcgcgggccg
     1501 cgtggccctg ccggtgccga gattcgggct ctgggcggtg gattcgctga gacgcgcaac
     1561 tcgctacact gagctggatc gcgagcagtt ggactatctg agctacggcc gagtgatgga
     1621 caccgcgcgg atgcgcaatg aattaggcta taacccgaag tggaccacga cggaagcttt
     1681 tgacgattac gtacgtggtc gtggattgac tccgatcgtt gacccacgat gggtacgctc
     1741 attggagagt cgcgccgtat cggcggcgca gcgttgggga cgttgaacga agactcgaaa
     1801 tcgggtgggg agaaggtatc gacgtggcgg gcgagtctaa agcgaaagtc attccgctgc
     1861 actcgaatac gggtcggcag gcggcgcagc ggcgggcagc ggcccgtggc gaaggcgcgc
     1921 gccggcatcc ctctctgctg agtgatccca acagccgtgc gtcggccgac cagatcgccg
     1981 cggtcgtgcg cgagatcgac gaacgtcgct ctgctgcggc aggcacgccg cccgccgacg
     2041 agtcgccgaa cgaactcgct cagcgaatcg cctcggttgc tgaattcatt cgtaagcgga
     2101 tgacgggcga ttacaccgtc gacgaattcg gcttcgatcg gcacctcaac aattcggtct
     2161 tcttgccttt gctgcgtgtg ctgttcaact cgtggttccg ggttgaggtc agcggcatcg
     2221 agaacctgcc ggaaacgggc gccgcactgg tggtggccaa ccacgccggt gtgctgccga
     2281 tggacgggct gatggtgtcg gtggcagtgc acgacaagca ccccgcacag cgggatctgc
     2341 ggctgctggc cgccgacatg gtgttcacca tgccggtcat gggtcaggcg gcacgcaagg
     2401 cgggccacac catggcatgc acctccgacg cgcaccggct gctcgatgcg ggcgagctga
     2461 ccgccgtgtt ccccgaggga tacaagggtc tgggcaagca cttcaacgac cgctacaagc
     2521 tgcagcgatt cggtcgcggc gggttcgtct cggcggcgct gcgcgccaag gcgccgatcg
     2581 tgccgtgctc gatcgtcggg tccgaggaga tctacccgat gatcgccgac gtgaagctgc
     2641 tggcccggct gttcgggctg ccttacttcc cgatcacccc gctgttcccg ttggcgggcc
     2701 ccgcgggcct gttgccgctg ccgtcgaagt ggcacatcga gttcggcgag gcgatcgaga
     2761 ccgccgacta cgacgacgcc gcggccgacg acccgatgat cacattcgag ttgaccgacc
     2821 aggtccgcga aaccatccag cagactcttt atcagttgct ggccaaccgc aggaatatgt
//
LOCUS       DASYLX010000159         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1433451,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000159 DASYLX010000000
VERSION     DASYLX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433451"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGP27_03815"
     CDS             <1..808
                     /locus_tag="VGP27_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV7850477.1"
                     /translation="RPGVAGTSVLVPGGPFVLGVDAVTEPQSLDNERSAHVVDVPGFR
                     IGRVPVTNGEWRQFVDDGGYREPRWWSLRGWSHREQAGLTAPQFWNAGHLAGTRTRFG
                     HLEDIPADEPAAHVTYFEAEAYAAWAGARLPTEMEWEKACAWDPEAGARRRFPWGASE
                     PTDALANLGGDSLRPAPVGAYPAGASAYGVEQMLGDVWEWTSSPLRPWPGFRPMLYEQ
                     YSQPFFDGDYKVLRGGSWAVAADILRPSFRNWDHPVRRQIFSGVRLAWDV"
     gene            808..1503
                     /gene="egtC"
                     /locus_tag="VGP27_03820"
     CDS             808..1503
                     /gene="egtC"
                     /locus_tag="VGP27_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HEV7850478.1"
                     /translation="MCRHLGWLGESRSVASLTLDPPYGLLVQSYSPRRQKHGLMNADG
                     WGVGFFDEHGVARRWRSAAPLWGDASFASVAPALRSGCVVAAVRSASVGMPIEASASA
                     PFSDGRWLLSHNGLVDRAVLAASSAAESTNDSALLAALIFERGLESLGDTITAVAASD
                     PNARLNILAGNGSELRATTWGDTLFALRRHDGVVLASEPFDDDKDWQEIPDRHLVVVS
                     DSRMELTPLKGSA"
     gene            1500..2465
                     /gene="egtD"
                     /locus_tag="VGP27_03825"
     CDS             1500..2465
                     /gene="egtD"
                     /locus_tag="VGP27_03825"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEV7850479.1"
                     /translation="MTLSLSNHLAADSAARALRRDVYDGLTQTPKSLPPKWFYDSAGS
                     DLFDQITRLPEYYPTRAEAEILRDRSAEIAAASGADTLVELGSGTSEKTRLLLSALRD
                     MGSLRRFVPFDVDAGMLQAASAAILDEYPGIEIDAVCGDFEEHLGKIPRVGRHLVVFL
                     GSTIGNLTKGPRAEFLSALSATLQPGDTLLLGTDLVKDTGRLVRAYDDSAGVTAQFNR
                     NVLAVVNRELDADFDLAEFEHVARWNADEERIEMWLRARSAQRVRVAALDLTVDFASG
                     EEMLTEVSCKFRQEGVAQELAAAGLERTHWWTDRAADFGLSLSTK"
     gene            2462..3589
                     /gene="egtE"
                     /locus_tag="VGP27_03830"
     CDS             2462..3589
                     /gene="egtE"
                     /locus_tag="VGP27_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis PLP-dependent enzyme
                     EgtE"
                     /protein_id="HEV7850480.1"
                     /translation="MTELADRWRAARPKVAGLHLDSGACSRQSFAVIDAAAQHARHEA
                     EVGGYVAAAAAAPVLDAGRSAVAALAGMTAADVVYTTGANHAFDLLLSSWTGTRTVAC
                     LPGEYGPNLAVMAANGFDVRVLPVDGAGRLAVDEAAAFLAASRPGLVHLTSLASHRGI
                     VQPARELAEVCRGLELPLVIDAAQALGHIDCAVGADAIYSSSRKWLAGPRGVGVLAIR
                     PALVERLRPRLPPPEWNLPLTVMERLEHGETNLAARVGLSVALGEFLAAGAEVIRTAL
                     AQQGRVTRDALADVKGWRVVEPADEPTAITTLVATNGADPQQVRAWLIAERAIVTTYA
                     EVARAPFEMAAPVLRVSPHVDSTPDELATFAEALKAATAQV"
     gene            3639..5027
                     /locus_tag="VGP27_03835"
     CDS             3639..5027
                     /locus_tag="VGP27_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEV7850481.1"
                     /translation="MSEHVDTEREQPAKKEKRDRTHWLYILVIIGVIAGITVGLLAPD
                     TGKSLGVLGELFVKLIKMMIIPVIFCTVVLGIGKIRAAAVGKVGGLALLYFLVMSTVA
                     LGIGLVVGNIIAPGTGLNIPADPGKGAELAGEAASEGGPMDFISDIIPTTLFSSLTAG
                     NVLQALFVAILVGFGIQTLGPAGAPLLRGVELLQKLVFRILIGVLWTAPIGAFGAIAE
                     VVGATGFEAVVQLAVLMLAFYLTCLIFVFGVLGAVLWTVSRVSIFKLVRYLAREYLLI
                     VATSSSESALPRLIAKMEHAGIKPATVGIVVPTGYSFNLDGTAIYLTMASLFIADAMG
                     TPMSIPQQLGLLAFMIIASKGAAGVSGAGLATLAAGLQSHRPDLLDGVGIIVGIDRFM
                     SEARAVTNFSGNAVATVLVGTWTSSVDGDRLRNVLDGDIPFDERTMIDDGDTEDDAST
                     DEKEPATAGAGH"
     gene            complement(5021..5467)
                     /locus_tag="VGP27_03840"
     CDS             complement(5021..5467)
                     /locus_tag="VGP27_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850482.1"
                     /translation="MRTVWAALPVVAVLAVALMACQTVRKADADPVEFTLGKEFVLGA
                     GQEAVSGGDDLRVRFSEVLEDSRCPTEVECFWTGQARIAVLIEPVGRPATTADFNTNP
                     APGQNAQIVRADDYTAELKSLDPYPRTPDEPISFEDYRATLLVQRQ"
     gene            complement(5464..>5941)
                     /locus_tag="VGP27_03845"
     CDS             complement(5464..>5941)
                     /locus_tag="VGP27_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV7850483.1"
                     /translation="NRVLPAGPTVIDMMANSAFYYGVLRSLSDDDRPLWTKMSFAAAH
                     QNFLEAAQNGMDARLYWPGLGEVTPDELVLRQLLPMAREGLRSWGVSDGVSDRYLGVI
                     EGRAKTGRNGSVWQVETVRALQKRGMARPAALAEMLHLYCGRMHSNEPVHTWEVDA"
BASE COUNT          908 a         1985 c         2057 g          991 t
ORIGIN      
        1 caggcccggt gtggcaggca cgtcggtgct ggtgcccggc ggcccgttcg tcctcggcgt
       61 cgacgccgtg accgaaccgc agtcgctcga caacgagcgc tcggcgcacg tcgtcgatgt
      121 gcccggcttc cgcatcggcc gggtgccggt gaccaacggt gaatggcgac agttcgtcga
      181 cgacggcggt taccgcgagc cgcggtggtg gtcgctgcgc ggttggtcgc atcgcgagca
      241 ggccggattg acggcgccgc agttctggaa cgcaggccat cttgcgggca cccgcacccg
      301 gttcggccat ctcgaggaca tccccgccga cgagcccgca gcgcacgtca cgtacttcga
      361 ggcggaggcc tatgcggcct gggcaggcgc gcgccttccc accgagatgg aatgggagaa
      421 ggcgtgcgca tgggatccgg aagccggcgc tcggcgccgc ttcccttggg gcgcatccga
      481 acccaccgat gcgctggcca atctgggtgg cgactcgcta cgccccgctc cggtcggcgc
      541 atatccggca ggggcgtcgg cctacggcgt cgagcagatg ctcggcgatg tctgggagtg
      601 gacgtcgtcg ccgttgaggc cgtggcccgg attcagaccg atgctctacg agcaatacag
      661 ccagccgttc ttcgacggcg actacaaggt gctgcgcggc gggtcgtggg ccgtcgccgc
      721 cgacatcctg cggccgagct tccgcaactg ggaccacccg gttcgcaggc agatcttcag
      781 cggtgtccga ttggcctggg acgtctgatg tgccgccacc tcggctggct gggcgagtcg
      841 cgttcggtgg catcgctgac tctcgaccct ccgtatgggc tactggtgca gtcgtattcg
      901 ccgcgtcggc agaaacacgg cctgatgaac gccgacggct ggggcgtcgg gtttttcgac
      961 gagcacggtg tggcccggcg ctggcgcagc gccgccccgt tgtggggcga cgcgtcgttc
     1021 gcgtcggtgg ccccggcgct gcgcagcggc tgcgtggtcg cggcggtcag atcggcgagc
     1081 gtcggcatgc cgatcgaggc gtcggcgtcg gcgccgttca gcgacggccg ctggctgctg
     1141 tcgcacaacg gcctggtgga tcgagccgta ttagcggcat cgtcggccgc ggaatcgacc
     1201 aacgacagcg cactgctggc cgcgttgatc ttcgaacgcg gcctggaatc gctcggcgac
     1261 acgatcaccg cggtcgccgc atccgatccg aacgcccggc tgaacatctt ggcgggcaac
     1321 ggttctgaac tgcgggccac cacgtggggt gacacgttgt tcgcgctgcg gcgacatgac
     1381 ggtgtggtgc tggcgagcga accgttcgac gacgacaagg attggcagga gatccccgac
     1441 cgtcacctcg tggtggtcag tgactctcgc atggagttga ccccgctgaa aggatcggca
     1501 tgacgctgtc gctgtccaac catctcgccg cggactccgc cgcgcgggca ttgcgccgcg
     1561 acgtgtacga cggcctgacg cagacaccca aatcgttgcc gcccaagtgg ttctacgact
     1621 ctgcgggaag cgacctgttc gatcagatca cccggctacc ggagtactac ccgaccaggg
     1681 cggaggccga gatcctgcgc gacaggtcag ccgagatcgc cgccgcatcc ggtgccgaca
     1741 cgctggtgga gctcggcagc ggcacctcgg agaagaccag gctgctgctc agtgccctac
     1801 gcgacatggg atcgctgcgc cggttcgtcc cgttcgacgt cgatgccggg atgctgcaag
     1861 ccgcgagcgc ggccatcctc gacgagtatc ccggcatcga aatcgacgcg gtctgtggtg
     1921 atttcgagga gcacctgggc aagatccccc gggtcgggcg ccacctcgta gtgttcctcg
     1981 ggtccacgat cggcaatctg acgaaggggc cgcgggcgga gttcctgtcc gcgctgtcgg
     2041 ccacgctgca acccggtgac acgctgctgc tgggcaccga cctggtcaag gacaccggcc
     2101 ggttggtgcg cgcctacgac gacagcgcgg gcgtgacggc gcagttcaac cgcaatgtgc
     2161 tcgcggtggt gaaccgtgag ctcgacgccg acttcgacct cgccgagttc gagcacgtgg
     2221 cccgctggaa cgccgacgag gagcgcatcg agatgtggct gcgcgcacgg tcggcgcaac
     2281 gggtgcgggt cgcggcgctg gatctgactg tcgacttcgc ttcgggcgag gagatgctca
     2341 ccgaggtgtc atgcaagttc cgccaagagg gtgtggcgca ggagctggcc gccgcgggct
     2401 tggagcgcac acactggtgg accgaccggg cggcggactt cgggctgtcg ctgtccacca
     2461 agtgaccgaa ctggcggacc ggtggcgggc cgcccggccg aaagtcgccg ggctgcacct
     2521 cgacagcggc gcctgctcgc gtcagagttt cgcggtcatc gacgccgcgg ctcagcacgc
     2581 gcggcacgag gccgaggtgg gtggctatgt cgcggcggcg gcagctgcgc ccgtcctcga
     2641 cgcgggccgc tcggcggtgg cagcgctggc tgggatgacg gccgctgacg tcgtctacac
     2701 caccggcgcc aatcacgcgt tcgacctgct tttgagcagc tggacgggga cgcgcaccgt
     2761 cgcctgcctg cccggcgagt acggccccaa cctcgcggtg atggcggcga acgggttcga
     2821 cgtgcgggtg ctgcccgtcg acggtgcggg acgactggcc gtcgacgagg ccgcggcgtt
     2881 cctggcagcc agccggccgg ggcttgtgca cctgacgtcg ctcgccagcc atcgcggcat
     2941 cgtccagcct gcccgtgagc ttgccgaggt gtgccgcggg ctcgaactgc cgttggtgat
     3001 cgacgccgct caggccctcg ggcacatcga ctgtgcggtg ggcgccgacg cgatctactc
     3061 gtcgtcgcgc aagtggctcg cgggcccacg cggcgtaggc gtgcttgcga ttcggcccgc
     3121 actggtcgag cggcttcggc cgcggctgcc accacccgag tggaacctgc cgttgacggt
     3181 gatggagcga ttggaacacg gcgaaaccaa tcttgcggcg cgcgttgggc tttcggtagc
     3241 gctcggcgag tttctggcgg cgggcgcgga agtcatccgc accgcgctgg cgcagcaggg
     3301 tcgagtgacc cgcgacgccc tggcggacgt caagggctgg cgggtcgtcg agcccgccga
     3361 tgagccgacc gcaatcacca cgctggtggc cacgaacggc gcggatccgc agcaggtccg
     3421 agcgtggctg atcgccgagc gcgcaatcgt caccacctac gccgaagtgg cgcgcgcacc
     3481 cttcgagatg gccgcgccgg tcctacgggt gtccccgcac gtcgacagca cgccggacga
     3541 actggcgaca ttcgccgaag ccctgaaggc cgcgaccgca caggtgtgac acagcacact
     3601 tctggttacc ccacaggaac gacacgaagg ggccgaccat gtccgagcac gtcgacactg
     3661 agcgcgaaca gccagcgaag aaggaaaagc gcgaccgcac tcattggctc tacatcctgg
     3721 tcatcatcgg cgtgatcgca ggcatcaccg tcggtctgct ggcaccggac accggtaaat
     3781 cgctgggcgt cctgggtgag ttgttcgtca agctgatcaa gatgatgatc atcccggtca
     3841 tcttctgcac ggtcgtcctc ggcatcggca agatccgggc ggccgccgtc ggcaaggtcg
     3901 gcggtctcgc gctgctgtat ttcctggtta tgtccacggt cgcgctgggc atcggtctgg
     3961 tcgtcgggaa catcatcgcg cccggaaccg gcctcaacat ccccgccgac ccgggcaagg
     4021 gcgccgagct ggcaggcgag gccgcgtcgg agggcggccc gatggacttc atctccgaca
     4081 tcatcccgac gacgctgttc tcgtcgctga cggcgggcaa cgtgctgcaa gcgctgttcg
     4141 tggcaatcct ggtcggcttc ggaatccaga cgctgggccc tgcgggcgca ccgctgctgc
     4201 gcggcgtcga gctgttgcag aaactggtct tccgcatcct gatcggtgtg ctgtggacgg
     4261 cgcccatcgg cgcattcggg gcgatcgccg aagtggtcgg tgccaccgga ttcgaggcgg
     4321 tcgtccagct cgcggtgctg atgctggcgt tctatctgac ctgcctcatc ttcgtattcg
     4381 gtgtgctcgg cgcagtgctg tggacggtca gccgggtgtc gatcttcaag ttggtccgtt
     4441 acctggcccg cgagtacctc ttgatcgtgg cgacgtcgtc gtcggagtcg gcgttgccac
     4501 ggctcatcgc aaaaatggag cacgcgggaa tcaagccggc gacggtggga atcgttgtgc
     4561 cgaccggcta ttcgttcaac ctggatggca ccgcgatcta cctgacgatg gcgtccctgt
     4621 tcatcgcgga cgccatgggc accccgatgt cgatacccca gcaactcggt ctgctggcgt
     4681 tcatgatcat cgcgtcgaag ggtgctgcgg gggtcagcgg cgctgggctt gcgacgctgg
     4741 ccgccggcct gcagagccac cgtcccgacc tgctcgacgg tgtcggcatc atcgtcggca
     4801 tcgaccggtt catgtccgag gcccgcgccg tcaccaactt ctcgggtaat gccgtggcaa
     4861 ccgtgttggt cgggacgtgg acgagctcgg tggacggcga tcgattgcgg aacgtgctcg
     4921 acggcgacat tccgttcgac gaacgcacca tgatcgacga cggcgacacc gaggatgacg
     4981 cgtctaccga cgagaaggaa ccggcgacgg ccggcgccgg tcactgacgc tgcaccagca
     5041 gcgtcgcccg gtagtcctcg aacgagatcg gctcgtcagg tgtgcgcgga tacgggtcca
     5101 gcgacttcaa ctcggccgtg tagtcgtcgg cacgaacgat ctgcgcgttc tggcccggcg
     5161 ccgggttggt gttgaaatcc gctgtcgtag caggtcttcc gacgggctcg atcagcacgg
     5221 cgatgcgcgc ctgcccggtc cagaagcact cgacctcggt cggacagcgg gagtcctcga
     5281 gcacctcgct gaaccgcacc cgcagatcat cgccgcccga cacggcttcc tgtcccgcgc
     5341 cgagaacgaa ctccttgccg agcgtgaact ccaccgggtc ggcgtcggcc ttgcggaccg
     5401 tctggcacgc catcagggcg acggccagca ccgcgacgac gggcaacgcc gcccacaccg
     5461 tcctcacgcg tcgacctccc aggtgtgcac cggttcgttg ctgtgcatcc gcccgcagta
     5521 gagatgcagc atctcggcca gcgccgccgg ccgggccatg ccgcgcttct gcagtgcccg
     5581 caccgtctcc acctgccaaa ccgagccgtt gcggcccgtc ttggcgcggc cctcgatgac
     5641 gccgagatac cggtcggaca ccccgtcgga gacaccccag ctgcgtaggc cctcgcgtgc
     5701 catcggcagc agttgacgca gcacgagttc gtcgggtgtc acctcgccca agccgggcca
     5761 ataaagccgg gcgtccatgc cgttctgcgc cgcttcgagg aaattctgat gtgccgcagc
     5821 gaaactcatc ttcgtccaca gcggacgatc gtcgtccgac aggctccgca acaccccgta
     5881 gtagaacgcc gagttggcca tcatgtcgat gacggtcggg cctgcgggca gcacgcggtt
     5941 c
//
LOCUS       DASYLX010000160        14156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3737919,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000160 DASYLX010000000
VERSION     DASYLX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737919"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VGP27_03850"
     CDS             <1..836
                     /locus_tag="VGP27_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7850484.1"
                     /translation="PGGRETGDALVRHPGVDKIAFTGSSATGRHIAAACGEQLKRVSL
                     ELGGKSAAIVLDDADLDHTVKHLKMAGLMNNGQACVAQTRILVSERRHHEVVDALAEM
                     VSGLTVGDPADESTDIGPLVAQRQQRRVVGFIQSGIDEGAKVVVGGPDKPYGRGWYVR
                     PTLFADATNDMKIAREEIFGPVLTVLRYHDEADAIRIANDSDYGLAGSVWTADVAHGL
                     RIAAEIRTGTYGINMYQLDTSAPFGGFKQSGIGREFGPEGLSQYTEVQSTVTAGELPP
                     S"
     gene            complement(833..1276)
                     /locus_tag="VGP27_03855"
     CDS             complement(833..1276)
                     /locus_tag="VGP27_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV7850485.1"
                     /translation="MSSYRGLAWSDVTVPTDLAEVVDEISYQRVVMNPGATWDYFPGH
                     FDPRYAESHGHPSIFVNTMHLAGFIDRIATDWAGPYSRVVRRKVSMVGSVYAGDSMVG
                     RGRVVDKRCDTTGDTLRHLVDLEIAIFNQRDELCCPAEVTLELAD"
     gene            complement(1273..1803)
                     /locus_tag="VGP27_03860"
     CDS             complement(1273..1803)
                     /locus_tag="VGP27_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850486.1"
                     /translation="MTAGIRYEFAFGTYEEALRMVGVRTEPRFAGTAVSAARIQHFAA
                     MVQDGNPAYWDIEFATEVWGGLVAPPAMLMGWLIPPPWLPNGERPVPSIAIRVPLPGT
                     TFINASNDVELVTPILEGDRLNVVEEVVSVSPEKRTKVGVGHFVETCDSYYRERGPLV
                     ATNRNTLFRFTPPETP"
     gene            complement(1808..2221)
                     /locus_tag="VGP27_03865"
     CDS             complement(1808..2221)
                     /locus_tag="VGP27_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850487.1"
                     /translation="MTMTYQQEFVLAAVAGRAAILNLNARHNRLFSAGDFVGWVATFR
                     HAGATVVRSGETFTNLRDAFDGGNGQRLVTVDHDITVDGVDATQTCVALLFLGSELRS
                     TGVYRDRLVYERGGWYYTSRELEWDVVPSKDALPV"
     gene            complement(2236..3864)
                     /locus_tag="VGP27_03870"
     CDS             complement(2236..3864)
                     /locus_tag="VGP27_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850488.1"
                     /translation="MNRTRRRRVPEGGRPDGPIGPSQFGVERFTIPDVLDQRAEQFGD
                     RTMMSIGGTPVTFAEMRDRSCAAANLLSDNGVARGDTVALFTATCPEWVYFWLGAARI
                     GAVTAAVNIANKDEYLIHALRLSRAKVVITDAERLLRLSEVVARVETVKTTLVIGHSL
                     TDALASASTKTPDDPLGPEELAALFFTSGTTGPSKAVATTWHYLFSAAAGVVTAWELQ
                     AGDVVWSAMPLFHLSAAPSVLAPLLVGSATVLSPTFRPSEVWDRVRDCGAAGFAGAGA
                     MVSMLWNQPPDPRDAQLPLRFISAAPISADMYRSVEQRYGARIVTMYGMTEAFPIAFK
                     SVSEEGVPGTSGRVNAAFDVRIVDGDGRAMPAGSVGEIACRPKSPYVMSDGYIDAAAT
                     GPDIVVAAHDEWFRTGDLGSLDSGGNLTYVDRVKDSLRRRGENVSSVEVETTVMRHPA
                     VLEAAAVAIPSELGEDDILVVVTLQPHADFDHADLLDFCSARMPYFCVPRYLEVLDEI
                     PKNVIGRVRKDLLRTRGLGDETWDREGHGYILSR"
     gene            complement(3833..4948)
                     /locus_tag="VGP27_03875"
     CDS             complement(3833..4948)
                     /locus_tag="VGP27_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7850489.1"
                     /translation="MTDTIDDHTIDPSEREFGPSGISLSKYRFPTGWFIVGFASELAP
                     GQVKRAHYFGEELVIFRTASGKVNVLDAYCLHLGANMGVGGTVEGEHIVCPWHGWQWR
                     GDGTNALIPYSKIGCKQNVRIKTYPAQEWYGFIVVWHERHGRAPYWQPPVLPELETDD
                     YYPLHPHSRMLNRVKVHAQMIIENAADPYHVQFVHKADSAANTTSFNADGYHLHATVT
                     ANFGGGRASTWLTPDGPVDADIIYDNYSLGLGIVRFPKELVATIEVTGQTPVDEDYTD
                     YFYTQASVREPGDTGDKPAGRAAKFLALQQEVIKQDFFTWENMKYLEKPNLAPEEARD
                     YAALRRWAHRFYPAEEPSPIDFGYTASGEPDPAAAGA"
     gene            5053..6291
                     /locus_tag="VGP27_03880"
     CDS             5053..6291
                     /locus_tag="VGP27_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850490.1"
                     /translation="MPMTTPVDLSDSALWRNGFPDELFVELRRERPLFHHALTDGVAK
                     TVKRDFWMATKHRHAQRIHRDTDSFTATDGPLIQGVGIVGSAPTIINMDPPELLKRRR
                     VMSHAFTPKAIGKLEEGIRRRAAAMVDGLLAAGGGDWISDVADVLPMSVIGDIIGIPE
                     QDRPDIFDTLDRILKVGSSEDPLAEQNAMELFGKIFTYALELTAEKRRNPTDDIWSTL
                     TSAVVTDENGETLSIPANELEIFFFVIAFAGSDTTKNALASGLQAFVANPEQIERYRE
                     DDAVRSSAVEEVLRWASPVTFWTRSTKVDVEMDGVTIPKGERVVAMLRSANRDEEVFG
                     DPFAFDIGRAENPHVTFGGGGPHHCLGAMLARAEIRAVLDELLCRADDISLGLPKVAY
                     PNLTNNMTIFDELPISLTPR"
     gene            complement(6364..8655)
                     /locus_tag="VGP27_03885"
     CDS             complement(6364..8655)
                     /locus_tag="VGP27_03885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7850491.1"
                     /translation="MSDGASTPLSDLRVVEVSDRIAGSYCGKLLVDAGADVVKVEPAA
                     GDPLRRFTATGGVPARGADAPLFSYLSAGKRSVTTISDELLACADVVIVTATRSAAAE
                     QGIDLARLLEARPDRVVVTISDFGWTGPWSDRPATEFTLQSDSGLTGFRGDPAGPPIS
                     VGGELGEYMGGAWAAYGAIALRRRVLGGGPGGHLDMSMLEAITLMQSSEWLHSQLLAR
                     PPVKRSVEVPSIEPAKDGYVGISMVTGQQWLDFAAMVDCPEFTEVPQLRFQIGRWDYR
                     DWIRERIDPWMRERTVEEIVELGQLFRLPLAPLGNGSTIPDMDHLRTRGVYVDNPAGF
                     RQPRAPWLMSVAGPAPVRAAPAVGEADGVSVWEARRSEPCARPPEAPLAGVRVVDFTA
                     FWAGPAASHSLGAFGADVIKIESIQRPDGIRYSGGMRTDVDDWWEYGWVFHAMNTNKR
                     SVTLDLQSEDGRKLVKDLVRQADVVIENFSPRVMEQFGLGADALLELNPKVVVVRMPA
                     FGLTGPWRDRVGFAPTMEQIAGLAWVTGLPDGPPVAPRGACDPLAGAHAAFATLAALE
                     FADRTGSGQLVEVPMIEIVLNVTAVQTIEFEIFGSVMERRGNRGHTAAVQDVYRCAGE
                     DSWVAVAVRPGDRDALASVIGEIGIEEWLAMQNAGAAVETLAGAGIPAAVVISPSLVT
                     ENPQLVHRGFFERLEHPRTGEGFYPSPPFARLDGAERWLLRTPPTLGQHNEEVLTELC
                     GLTAADLARLTADGVIGTRPKGA"
     gene            8763..9572
                     /locus_tag="VGP27_03890"
     CDS             8763..9572
                     /locus_tag="VGP27_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEV7850492.1"
                     /translation="MTTLGIGPEARRRLGARRTAEIVADELRRQIIDGELTDGDLLPR
                     QEILVEQFNVSLVSLREALRILETEGLVSVRRGNRGGAVVHAPAKASAAYMLGLLLQS
                     DTVPLADLGTALQELEPMCATLAARRPDRGETLIPKLRENNDSMAEHIEDGAKFTEIG
                     GQFHDEVVRGCGNHTMIAVVGSLETLWTGHLNIWAKETAARGEYPSMSKRRIALTVHT
                     KLTDAIEAGDGERARKLAARHIADTQSYFSAGDPEQRIVALSPQALARRQR"
     gene            9583..10302
                     /locus_tag="VGP27_03895"
     CDS             9583..10302
                     /locus_tag="VGP27_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850493.1"
                     /translation="MRTALITGGSGGIGRACGRKLADLGYDVVLTARREGPLRAAADE
                     MGARYVVADASDPAEFAPAVEAAGDIDLLVHASGVLGGTYARKQTFDQWRATMSANLD
                     SCFVVTSAALPRMKPGSRFVFISSSAAHEPMMARTAYSASKAGMNAFAGALAQEVDRD
                     GINVHIVTPGPVETEMLQDVPFEMWAIRTADVADAVAWLDTLDPSVDLPEIRLHAITR
                     GPSARAPIVPLEAGRRATRTK"
     gene            complement(10308..11447)
                     /locus_tag="VGP27_03900"
     CDS             complement(10308..11447)
                     /locus_tag="VGP27_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850494.1"
                     /translation="MTITERTEEAVGRDIAVTIVDTDVHPLPVSGDVLKSYAPSEWKD
                     TLWPTGNAVSPMSYFYDTPDAYKTSSLRMDASPPVGGPAGSDPEFAAKQLLVDAGVSI
                     AVLEPMCDAQLPQAEHVLKSTYNDWLADVWLGENNWHGRWRGAISVTAQDPGQAAREV
                     ERWAGHPYMAEVLMTPQTRGIPFGSPHFDPMYEAAARNDLPVATHLMGQTPYELIPIF
                     PVGNPAHWHDFFASWPLLYVSHLMSLVFDGAFDRHPDLQVVFVEGGFTWAMPVMSRMD
                     RIWEYRKADLPHVRRKPSDYVREHVRFTTQPLEEVDVTVYRDYIEMMDLGDNIMFSTD
                     YPHWSYDAPDWAIRRFPADQRERIMRGNATALYGLPSSVKSLPGE"
     gene            complement(11444..12529)
                     /locus_tag="VGP27_03905"
     CDS             complement(11444..12529)
                     /locus_tag="VGP27_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850495.1"
                     /translation="MTVIDANVQPNFRYNSEIRRYLSPAHKLRAIPDVEQQWYQAPGG
                     DYRDDLYGDGYPGSDPETVARHLFGDGGSAFGSSPKRLIADYAILNPLTRGNIADYLL
                     NSRICAAVNDWLLDRWLEPDATARFRGTIRVNPEDVRGAVAEIERLADHPKMVQVGVP
                     LQSREPYGKPVFEPIWEAAAAHGLPVSVHINGGNGVDYAPTFAGHASTYPGYAAFMPL
                     NSFVHLATLIIEGTFSRHPDLTFVFADGGYDLLTPLMWRLDTFWMSMRDQTPWVDRYP
                     SEYLTDHVRYCTSALDGPTDEDQAERWLDFTGKADLLMYGSGYPHWSASTPDAAVAGL
                     ASEQRQNVLWRNASDVYGIAAVEGSLT"
     gene            complement(12591..12947)
                     /locus_tag="VGP27_03910"
     CDS             complement(12591..12947)
                     /locus_tag="VGP27_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7850496.1"
                     /translation="MQRRPVCAIEDLPPGSMKLVQAGKFGVGVYNIRGSLYAIANYCS
                     HEGAPLCAGYVGGTTEFAPDAPGRVRYVREGQVARCPWHNWEFDITTGRNLADPSKRV
                     RTYEVDVADGQVYLTA"
     gene            13039..>14156
                     /locus_tag="VGP27_03915"
     CDS             13039..>14156
                     /locus_tag="VGP27_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850497.1"
                     /translation="MTLSTDSHDKPTAPDIETDPYGFFEHMRNTAPVWRGTLMESDLM
                     PDELKVSENWTLFDFESVFAAFREDTVFASEMYNNTIGLVFGPTILGMHGKQHHDHRS
                     LVSKAFKQSALTKWEPEVIDPICDQLVDEFKNDGGADLVKALTFEFPTRVTAALLGLP
                     EQDLEMFRRLSLDLISITDDIEAGLTASVELGTYFQEQVDQRRSAMTDDVIGDLVAAE
                     IDGEKLTDEAIISFLRLLLPAGLETTYRSSGNLLQLLLTHPDQLDTLQQDRSLIPAAI
                     EEGIRYETPLVLVMRNTTRDVELHGMTIPEGAQVNLCMGSANRDDKRWENPDVFDIQR
                     PRRAHISFAGGIHSCLGMHLARVETKAMLTSLFDRVTDL"
BASE COUNT         2493 a         4712 c         4557 g         2394 t
ORIGIN      
        1 tgcccggcgg ccgggaaacc ggcgatgcgc ttgtccgcca ccccggtgtg gacaagatcg
       61 cgttcaccgg ctcctcggcg accggcagac acatcgccgc cgcgtgcggc gagcaactca
      121 aacgggtcag cctcgaactc ggcggcaagt cggcggccat cgtgctcgac gacgccgacc
      181 tcgaccacac ggtcaaacac ctcaagatgg ccggcctgat gaacaacggg caggcatgtg
      241 tggcacagac ccggatcctg gtcagcgaac gcaggcatca tgaggtcgtc gacgcgctcg
      301 ccgagatggt gtccggtctg accgtcggcg acccggccga cgagtcgacc gacatcggcc
      361 cactcgtcgc ccagcgtcag cagcggcgcg tcgtgggctt catacagtcc ggcatcgacg
      421 aaggcgccaa agtcgttgtg ggcgggcccg ataagcccta tggccgtggc tggtacgtcc
      481 ggcccacctt gttcgccgac gcgacgaacg acatgaagat cgcccgcgag gagatcttcg
      541 gccccgtgct gacggtgctg cgctaccacg acgaagccga tgcgatcagg atcgccaacg
      601 acagtgacta cggactggca gggtcggtct ggacggcgga tgtcgcacac ggtctgcgaa
      661 tcgcggccga aatccgcacc ggcacctacg ggatcaacat gtatcagctc gacaccagtg
      721 cgccgttcgg cgggttcaag caatccggca tcggcaggga attcggcccg gaaggcctct
      781 cgcagtacac cgaggtgcag tcgacggtga cggcaggcga gttgccgccg agctagtccg
      841 ccaactccag ggtcacctcg gcggggcagc acagttcgtc gcgctggttg aagatggcaa
      901 tctcgaggtc gaccaggtgg cgcagtgtgt caccggtggt gtcgcagcgc ttgtcgacca
      961 cacggccgcg gccgaccatg gagtcgccgg cgtatacgga tccgaccatc gacaccttgc
     1021 gccgcaccac gcggctgtac ggacccgccc agtcggtcgc tattcggtcg atgaagcccg
     1081 cgagatgcat tgtgttgaca aagatcgagg gatgtccgtg actttcggcg taccgcgggt
     1141 cgaaatggcc tgggaagtag tcccacgtcg cacccgggtt catcaccacg cgctggtagc
     1201 tgatttcgtc aacgacctct gcgaggtcgg tcggcactgt gacgtcgctc cacgccagtc
     1261 cgcggtatga actcacggcg tctccggagg agtgaagcgg aacaacgtgt ttcggttggt
     1321 cgctaccagc ggcccccgct cacggtagta gctgtcgcag gtttcgacga aatgaccgac
     1381 gccgaccttg gtgcgcttct cgggggacac cgaaaccact tcttcgacca cgttgaggcg
     1441 gtcgccctcg aggatcgggg tcacgagttc gacgtcgttc gatgcgttga tgaacgtggt
     1501 gcccggtagc ggcacgcgga tcgcgatcga gggaacgggc cgctctccgt tgggcagcca
     1561 cggcggcggg atgagccagc ccatcagcat cgcgggcggc gcgaccagcc caccccacac
     1621 ctccgtagcg aactcgatgt cccagtacgc cggatttccg tcctgaacca tcgccgcgaa
     1681 atgctggatg cgcgccgcgc tcaccgcggt acccgcgaac ctaggctcag tgcgcacgcc
     1741 taccatgcgc agggcctcct cgtaggtgcc gaacgcgaac tcgtagcgga ttcccgcggt
     1801 catcggctca taccggtagt gcgtctttgc ttggcacgac gtcccattcg agttcgcgtg
     1861 atgtgtaata ccatccgccg cgctcgtaaa cgaggcggtc gcggtatacg ccggtggagc
     1921 gaagttcgga tccgaggaac aggagcgcga cacacgtctg ggtggcgtcg acgccgtcga
     1981 ccgtgatgtc gtggtcgacg gtgacgagtc gctgtccgtt cccgccgtcg aaggcgtcgc
     2041 gcaggttggt gaaggtctcg cccgagcgga caacggtggc gcccgcgtgc cggaatgtcg
     2101 cgacccagcc gacgaagtcg cctgcggaga acaatcgatt gtgccgggcg ttcagattca
     2161 ggatggccgc gcgcccggcc actgcggcga gcacgaactc ctgctggtag gtcatcgtca
     2221 tgtgtttctc ccgtcctagc gactcagtat gtagccgtga ccctcgcggt cccatgtctc
     2281 gtcgccgaga ccccttgtgc gcagcaggtc tttgcggact cgcccgatga cgttcttcgg
     2341 tatctcgtcg agcacttcca gataccgcgg aacgcagaag tacggcatcc gggcagagca
     2401 gaagtccaaa agatcggcgt ggtcgaagtc cgcgtgcggt tgcagggtga cgacgaccag
     2461 gatgtcgtcc tcgcccaact cactcgggat ggcgacggcg gcggcttcca gcacggcggg
     2521 atggcgcatc accgtcgtct cgacttcgac cgaggacacg ttctcgccgc gccgccgcag
     2581 tgaatccttc acccggtcga cgtaggtcag gttgccgccg gaatccaggg agccgagatc
     2641 gccggtgcga aaccattcgt cgtgcgccgc gacgacaatg tcaggtcccg tcgcggcggc
     2701 atcgatgtag ccgtcgctca tcacgtaagg cgatttcggc ctgcaggcga tctcgccgac
     2761 cgagccggcc ggcattgctc gtccgtcgcc gtcgacgata cgtacgtcga acgccgcgtt
     2821 cacccgtccg gatgtgcccg ggacgccctc ctcggagacg ctcttgaacg cgatcgggaa
     2881 ggcctctgtc atgccgtaca tcgtcacgat cctcgcgccg tagcgctgct cgacgctgcg
     2941 gtacatgtcg gccgaaatgg gggccgcgga gatgaaccgc agcggcagct gcgcgtcacg
     3001 cggatcgggc ggctggttcc acagcatcga gaccattgcg cctgcaccgg cgaaacccgc
     3061 cgcgccgcag tcccgtacgc ggtcccacac ctcgctcggc cggaaggtcg gcgacaggac
     3121 cgtggccgac ccgacgagca gcggggccaa caccgatggc gcggcgctca gatggaaaag
     3181 cggcatggcg ctccacacca cgtcgcctgc ttgcagctcc cacgccgtca ccaccccggc
     3241 cgccgccgag aacaggtaat gccaggtcgt ggcgacggcc ttcgacggcc cggtcgtgcc
     3301 cgaggtgaag aagagcgccg ccaactcttc tggccccaac gggtcatctg gtgtcttcgt
     3361 cgatgcggac gccagcgcgt cggtcagcga atgcccgatg accagtgtgg ttttcacggt
     3421 ctccacccgt gcgaccacct cgctgagacg tagaagccgc tcggcgtcgg tgatgaccac
     3481 cttggcgcga gacagccgca gcgcgtggat caggtattcg tccttgttgg cgatgttcac
     3541 cgcggccgtc accgccccga tccgcgctgc gccgagccag aaatagaccc actcggggca
     3601 ggtcgccgtg aacaacgcga cggtgtcacc cctcgcgacg ccgttgtcgg acaacaggtt
     3661 cgccgccgca cacgagcgat cgcgcatctc ggcgaaggtc accggcgttc caccgatgga
     3721 catcattgtg cggtcaccga actgctcagc gcgctggtcc aacacgtccg ggatcgtgaa
     3781 gcgctcgaca ccgaactgcg acgggccgat cggcccgtcc ggcctaccgc cttcaggcac
     3841 ccgccgccgc cgggtccggt tcaccgctgg cggtgtaccc gaagtcgatc ggcgagggct
     3901 cttcggccgg atagaaccga tgcgcccatc gccgcagagc ggcgtagtca cgggcctcct
     3961 ccggggccag attcggcttc tccaagtact tcatgttctc ccacgtgaag aaatcctgct
     4021 tgatgacttc ctgttgcagc gcaaggaact tcgcagcgcg acctgccggc ttgtcaccgg
     4081 tgtcaccggg ttcgcggacc gaagcctggg tgtagaagta gtcggtgtag tcctcgtcca
     4141 ccggagtctg accggtcacc tcgatggtcg cgacgagttc cttggggaac cgcacgatgc
     4201 cgagccccaa cgagtagttg tcgtagatga tgtcggcatc gacggggccg tcgggcgtga
     4261 gccatgtcga cgcacggccg ccgccgaagt tcgcggtcac cgtcgcgtgc aggtgatagc
     4321 cgtcggcgtt gaacgacgtg gtgttggcgg cactgtcggc cttgtggacg aactgaacgt
     4381 gatacgggtc ggccgcgttc tcgatgatca tctgcgcatg caccttgacc cggttgagca
     4441 tccgtgaatg tgggtgcagc gggtaataat cgtcggtttc cagctcaggc aacaccggcg
     4501 gctgccagta gggcgcccgg ccgtggcgct cgtgccacac cacgatgaac ccataccact
     4561 cctgcgcggg ataggtcttg atcctcacgt tctgcttgca gccgatcttg ctgtacggga
     4621 tcagcgcgtt ggtgccgtca ccccgccact gccagccgtg ccaagggcaa acgatgtgct
     4681 cgccctcgac ggtgccgccg acgcccatgt tcgccccgag gtgaagacag tacgcatcga
     4741 gcacgttgac cttgccggat gcggtccgga aaatgaccaa ctcctcaccg aagtagtgcg
     4801 cccgcttgac ctggccaggc gccaactccg aggcgaagcc gacgataaac cagccggttg
     4861 ggaaccggta cttcgaaagg ctgataccgc tcgggccgaa ctcacgttcc gacggatcga
     4921 tggtgtgatc gtcgatcgtg tccgtcatgc cgtctccgtc cgcgcaatat ttgggcaggt
     4981 cagaccctgt ccgccgacta ttgttactat tttaagcatt ctaggtcaag gacagccgaa
     5041 ccaggagccc gcatgcccat gaccacacca gtcgacttgt cggattccgc tttgtggcgg
     5101 aacggttttc ccgacgagct gttcgtcgag ctgcgccgcg agcgccccct gttccaccac
     5161 gcgctgaccg acggcgtggc caagaccgtc aaacgggact tctggatggc caccaagcac
     5221 cggcatgcgc agcgcatcca ccgcgacacc gactccttca ccgcaaccga cggtccgctg
     5281 attcaaggtg tgggcattgt cggttccgcg ccgaccatca tcaacatgga tccgcccgag
     5341 ctgctcaagc gccgccgggt catgtcccac gccttcacac cgaaggcgat cggcaagctc
     5401 gaggagggca tccgaaggcg cgcggcggcg atggtcgacg ggctcctggc cgcgggcgga
     5461 ggcgactgga tctccgacgt cgccgacgtg ttgcccatgt cggtgatcgg cgacatcatc
     5521 ggcattcccg agcaggatcg gcccgacatc ttcgacacgc tcgatcgcat tctcaaggtc
     5581 ggatcctccg aggacccgct cgccgagcag aacgcgatgg agttgttcgg caagatcttc
     5641 acctatgcgc tcgagctcac cgcggagaag cggcgcaacc ccaccgacga catctggagc
     5701 actctgacgt ccgcggtcgt caccgacgag aacggcgaga cgctgtcgat cccggccaac
     5761 gagttggaga tcttcttctt cgtcatcgcc ttcgcaggca gcgacaccac caagaacgcg
     5821 ctggcctccg ggcttcaggc cttcgtcgcc aaccctgagc agatcgagcg gtatcgcgag
     5881 gacgatgcgg tccggtcgag cgcggtggaa gaggtgctgc gttgggcgtc gcccgtcacg
     5941 ttctggaccc gcagcacgaa ggtcgacgtg gagatggacg gcgtcacgat tccgaagggc
     6001 gagcgggtag tggcgatgtt gcgctcggcg aaccgcgacg aggaggtctt cggcgacccg
     6061 ttcgcgttcg acatcggccg cgctgaaaat ccgcacgtga cgttcggtgg cggtggaccg
     6121 caccactgcc tcggcgcgat gctggcccgc gccgagatcc gcgcggtgct cgacgaattg
     6181 ctctgccgcg cagacgacat cagcctcggt ctgccgaagg tggcttatcc caacctcacc
     6241 aacaacatga cgatcttcga cgagttgccg atctcgctca ccccacgctg acatacgccc
     6301 aaaccgacaa atgggcgcgt tctcgggctg attttgcgcc caaaccaaca aatgggcggc
     6361 tggttaagcg ccctttggcc gcgtgccgat gacaccgtcg gccgtcagcc gcgccagatc
     6421 ggccgccgtg agcccacaca gttccgtcag cacctcctcg ttgtgctggc ccagcgtcgg
     6481 cggtgtacgg agcagccacc gctcggcgcc gtccagacgg gcgaacggag ggcttggata
     6541 gaagccctcg ccagtgcgtg gatgctcgag ccgttcgaag aagccgcggt gcaccagttg
     6601 agggttctcg gtcaccaacg acggcgagat gacgacggcg gccggaatcc ccgctccggc
     6661 gagagtttcc acggccgcgc cggcgttctg cattgccagc cactcctcga tgccgatctc
     6721 gccgataact gaggccaatg cgtcgcggtc gccgggacgg acggctacgg cgacccagct
     6781 gtcctctccc gcacaccgat agacgtcctg caccgccgca gtgtgcccgc ggttgcccct
     6841 gcgttccatg accgagccga aaatctcgaa ttcgattgtc tgcaccgcag ttacgttgag
     6901 cacgatctcg atcatgggaa cctcgacgag ctgaccgctg cctgtccgat cggcgaactc
     6961 cagcgcagca agggtggcga acgccgcgtg tgctcctgcg agcggatcgc atgcaccacg
     7021 cggcgcgacg ggagggccgt ccggcaggcc ggtcacccag gccaagccgg cgatctgctc
     7081 catcgtcggc gcgaatccga cccggtcgcg ccacgggcca gtcaatccga atgcgggcat
     7141 tcgcaccacg acgaccttcg ggttcagttc gagcagggcg tcggcgccga gcccgaactg
     7201 ctccatcacc cgcggggaga agttctcgat cacgacatcg gcctggcgca ccaggtcctt
     7261 gacgagcttg cgtccgtcct cggattgcag gtcaagcgtg accgaccgct tgttggtgtt
     7321 catggcatga aacacccagc cgtactccca ccagtcgtcg acgtcggtgc gcatcccgcc
     7381 ggagtagcgg atcccgtcgg gacgctggat cgactcgatc ttgatgacgt cggcgccgaa
     7441 cgcccccagt gaatgcgacg cggcgggccc ggcccagaac gccgtgaaat ccaccacccg
     7501 cacgccggcg agcggtgctt ccggcggccg cgcgcacggc tccgatcgcc gtgcctccca
     7561 gacggatacg ccgtcggcct ccccgaccgc aggagcggcc cgcaccggcg cgggaccggc
     7621 aaccgacatc agccacggtg cgcgcggctg acggaagccc gcggggttgt cgacgtacac
     7681 cccgcgtgtg cgcaggtgat ccatgtcggg aatggtcgac ccgtttccga gcggagccag
     7741 cggtaaccgg aagagctgcc cgagctccac gatctcctcg acggtgcgct cacgcatcca
     7801 ggggtcgatc cgttcgcgga tccagtcccg gtagtcccac cggccgatct ggaaccgcaa
     7861 ttgcggaacc tcggtgaact cggggcagtc caccatcgcg gcgaagtcca gccactgctg
     7921 accggtcacc atgctgatcc cgacgtagcc gtccttggcc ggctcgatcg acggcacctc
     7981 gaccgagcgt ttgacgggtg gccgggccaa caactgcgag tgcagccatt cgctgctctg
     8041 catcaacgtg atcgcctcga gcatcgacat gtcgagatgc ccgccgggtc cgccgccgag
     8101 gacccgacga cgaagggcga tcgcgccgta ggcggcccat gcgccgccca tgtactcccc
     8161 cagctcgccg ccgacggaga tgggcgggcc agccgggtcg ccgcgaaaac cggtcagccc
     8221 ggagtcggac tgcagggtga actctgttgc gggccggtcc gaccacggcc cggtccagcc
     8281 gaaatcggag atggtgacga cgacgcgatc cggcctggcc tcgagcaacc gcgctagatc
     8341 gatgccctgc tcggcggccg cggatcgggt cgcggtgacg atcacgacgt cggcgcaggc
     8401 caacagttca tcggaaatgg ttgtgacgct gcgctttccg gcggacagat aactgaacag
     8461 cggcgcatcc gcgcctcggg ccggcacgcc accggtagcg gtgaagcggc gcaatggatc
     8521 gcccgcggcg ggctcgacct tcaccacgtc ggcgcccgca tccaccagca gcttgccgca
     8581 gtagcttcct gcaatacggt cgctgacctc aacgacgcgc agatcagaga gcggagtgga
     8641 tgctccgtca ctcacgcgca caacctccca gggccgtttc ttgtcatctt agttactttt
     8701 gtcggatagc gaccccgtag gccgcaggtg gcacgcctat cgctgttaac attgccattg
     8761 aaatgacaac actcggaatc ggccccgagg cccgccgccg gctcggagcc cgcagaacgg
     8821 ccgagatcgt cgccgacgaa ctccgccgtc agatcatcga cggcgagctc accgacgggg
     8881 acctgttgcc gcgccaggag atcctggtgg agcagttcaa cgtcagcctg gtgtcactgc
     8941 gggaagcctt gcgaattctc gaaaccgagg gtttggtctc ggtgcgccga ggcaaccgag
     9001 gtggcgcggt ggtacacgcg cctgccaaag cgagcgcggc ttacatgctg ggcctgctgt
     9061 tgcagagcga caccgtgccg ctggcggatc tcggcaccgc acttcaggaa ctcgagccga
     9121 tgtgtgcgac gctcgcggcg cggcgcccgg atcgtggtga aaccctgatc ccgaagctca
     9181 gagagaacaa cgactcgatg gccgagcaca tcgaggacgg cgccaagttc accgagatcg
     9241 gcggccagtt ccacgacgag gtcgtgcgcg gctgcgggaa tcacacgatg atcgcggtgg
     9301 tcggcagcct ggaaacgctg tggaccggtc acctcaacat ctgggcgaag gagaccgcgg
     9361 cgcgcggcga gtacccgtcg atgagcaagc ggcggatcgc tctgaccgtg cacaccaagt
     9421 tgaccgacgc gatcgaagcc ggtgacggcg agcgcgcccg taagctcgcc gcccgccata
     9481 tcgccgacac gcagtcgtac ttcagcgcgg gtgatcccga acagcgaatc gtggccctct
     9541 cgccgcaggc gctggcccgc cgccagcgct gaaaggtgct gtgtgcgtac ggcgttgatc
     9601 accggcggca gtggcggcat cggccgggcg tgcgggcgca agctcgctga cctcggctac
     9661 gacgtcgtgc tgaccgcgcg gcgggagggg ccgcttcgcg cagccgccga cgagatgggt
     9721 gcgaggtacg tcgtcgccga cgccagcgat ccggcggagt tcgcccccgc ggtggaggcg
     9781 gcaggcgata tcgacctgct cgtccacgca tccggcgttc tcggcggaac ttacgcccgc
     9841 aagcagactt tcgatcagtg gcgcgcgacg atgtcggcca atctcgactc gtgcttcgtc
     9901 gtcacctcgg ccgccctgcc acgaatgaag ccgggctcac gttttgtgtt catctcgtcg
     9961 tctgccgcgc atgaaccgat gatggcaagg acggcgtatt ccgcatccaa ggcgggcatg
    10021 aacgcgttcg cgggcgcgct ggcccaagag gtggaccgcg acggcatcaa cgtccacatc
    10081 gtgacccctg gtccggtcga aaccgagatg ctccaggacg ttcccttcga gatgtgggcg
    10141 atacggaccg ccgacgtggc cgacgccgtc gcctggctgg acacccttga cccgtcagtc
    10201 gacctgccgg agatcaggct gcacgcgatc acgcggggtc cgtcggcgcg ggcgccgatc
    10261 gtgccactgg aggctgggcg gcgcgccacc cggacgaagt gatctcgcta ttcgccggga
    10321 agcgacttga cgctcgacgg cagcccgtac agcgcggtcg cgttgccgcg catgatccgc
    10381 tcgcgttgat cggccgggaa ccgcctgatc gcccagtccg gtgcgtcgta gctccagtgc
    10441 gggtaatccg ttgagaacat gatgttgtcg ccgaggtcca tcatctcgat gtagtcgcga
    10501 taaacggtga cgtcgacttc ctcgagcggc tgggtggtga accggacgtg ctcgcggacg
    10561 taatccgacg gcttacgtcg cacgtgcggc agatcggcct tgcggtactc ccagatccgg
    10621 tccatccgcg acatcaccgg catcgcccag gtgaagccgc cctcgacgaa gaccacttgc
    10681 agatcgggat ggcggtcgaa ggcaccgtcg aacaccaggc tcatcaggtg tgacacgtag
    10741 agcagcggcc aggacgcgaa gaagtcgtgc cagtgggcgg ggttgccgac aggaaagatc
    10801 gggatcagct cgtacggtgt ctgacccata agatgcgttg cgacgggcag gtcattgcgc
    10861 gcggcggcct cgtacatcgg gtcgaaatgt gggctgccga agggtattcc gcgggtctgc
    10921 ggcgtcatca gcacctcggc catgtacggg tggcccgccc accgctcgac ctctcgcgcg
    10981 gcctgaccgg ggtcctgcgc ggtcacgctg atcgcgccgc gccagcggcc gtgccagttg
    11041 ttctccccga gccacacgtc ggccaaccag tcgttgtagg tggacttgag gacgtgttcg
    11101 gcctgcggca gctgcgcatc gcacatcggc tccagcaccg cgatgctcac cccggcgtcg
    11161 accagcagtt gtttggcggc gaactccgga tcgcttccgg ccgggccgcc gacgggcggg
    11221 ctcgcgtcca tgcgcaatga gcttgtcttg taggcatcgg gtgtgtcata gaagtacgac
    11281 atgggcgaga cggcgttgcc ggtcggccac agcgtgtcct tccactccga cggggcgtag
    11341 gacttgagca cgtcaccgga taccggcaac gggtgcacgt cggtgtcgac gattgtcacc
    11401 gcgatgtccc ggccgacggc ttcttcggtg cgctcggtta tggtcacgtc agacttcctt
    11461 ctactgccgc tatcccgtag acgtcacttg cgttgcgcca caacacgttt tgacgctgtt
    11521 cgctggctag cccggcgacg gcggcgtcgg gcgtcgaggc cgaccaatgc ggatagcccg
    11581 acccgtacat cagcagatcc gccttgccgg tgaaatccag ccaccgctcg gcctggtcct
    11641 cgtccgtcgg cccgtccagc gccgacgtgc aatagcgcac gtggtcggtc aggtactcgc
    11701 tcggataccg gtcgacccac ggggtttggt cgcgcatcga catccagaac gtgtccagcc
    11761 gccacatcag gggcgtgagt aggtcgtaac cgccgtcggc gaatacgaac gtcagatccg
    11821 gatgcctgct gaacgttccc tcgatgatca gcgtcgcgag gtggacgaac gagttcagcg
    11881 gcatgaacgc cgcgtagccc ggataggtgg aggcgtggcc cgcgaaggtc ggtgcgtagt
    11941 cgacgccgtt gccaccgttg atgtgcaccg acacgggcag tccgtgtgcg gcggccgcct
    12001 cccagatcgg ctcgaacacc ggcttgccgt agggctccct cgactgcagc ggaacaccga
    12061 cctgcaccat cttcggatgg tcggccagcc gttcgatctc ggcgaccgca cccctgacgt
    12121 cttcagggtt gacccggatc gtgccgcgga agcgtgcggt cgcgtcgggt tccagccagc
    12181 ggtcgagcag ccagtcgttg accgccgcgc aaatccggct gttcagcaga tagtcggcga
    12241 tgttgccgcg cgtcagcgga ttgagtatcg cgtagtcagc gatgagccgc ttgggcgagg
    12301 agccgaaggc agagccgccg tctccgaaca gatgacgcgc aacggtctcg gggtcggaac
    12361 ccggataacc gtcgccgtac aggtcgtcgc ggtagtcgcc gccgggcgcc tgataccact
    12421 gctgctcgac gtcggggatc gcgcgcagct tgtgcgcggg cgagagatag cgccggatct
    12481 ccgagttgta ccggaaattc ggctgcacat tggcgtcgat gacagtcatg cgccgacctc
    12541 gcaggcttcg cccgcgcatt ccgtgtcgat gattcgctcg caagcttcac tcatgccgtc
    12601 agatacacct gcccgtcggc cacatccacc tcataggtgc gcacccgctt gctcgggtcg
    12661 gccaggttgc ggcccgtggt gatgtcgaac tcccagttgt gccacgggca ccgtgcgacc
    12721 tgcccttcgc gcacataccg cacccggccc ggcgcatctg gcgcgaactc ggtcgttccg
    12781 ccgacatagc ccgcgcacag cggagcgccc tcgtgcgagc agtagttggc gatcgcgtac
    12841 agcgaaccgc ggatgttgta gacgcccacg ccgaacttgc cggcctgcac gagtttcatg
    12901 ctcccgggag gcaggtcctc gatcgcacac accggacgac gttgcatcac gtgtcccttc
    12961 aggtttcctt gacctaggat actaaaatag taaagatagg gcgaccgact cgctagacgc
    13021 tgctgtccgg gaggcactgt gacgctcagc acggattcac acgacaagcc gaccgctccg
    13081 gacatagaga ccgacccata cggtttcttc gagcacatgc gcaacacggc gccggtgtgg
    13141 cgcggcacgc tcatggagag cgatctgatg cccgacgagc tcaaagtctc cgagaactgg
    13201 actctgttcg acttcgagag cgtcttcgcc gctttccggg aggacaccgt gttcgcctcg
    13261 gagatgtaca acaacacgat cggtctagtc ttcgggccga cgatcctcgg catgcacggc
    13321 aaacagcatc acgaccatcg cagcctggtg agcaaggcct tcaagcagag tgcgctcacc
    13381 aagtgggaac ccgaggtgat cgacccgatc tgcgaccagc tcgtcgacga gttcaagaac
    13441 gacggcggtg ccgatctggt caaggcgctg acattcgagt tcccgacgcg cgtaacggct
    13501 gcccttcttg gcctgccgga gcaggacctg gagatgttcc ggcggctgtc gctggacctc
    13561 atctcgatca ccgacgacat cgaagccggc ctgacggcat cggtcgaact gggcacctac
    13621 tttcaggagc aagtggatca gcggcgcagc gccatgaccg acgacgtcat cggcgacctc
    13681 gtcgccgcgg agatcgacgg cgagaaactc accgacgaag cgatcatctc gttcctgcgc
    13741 ctgctactgc ccgcgggtct cgagaccaca taccggtcct cgggcaatct gctgcagctg
    13801 ctgctcacac atccggacca actcgatacg ttgcagcagg atcggagcct cattccggcc
    13861 gccatcgaag aagggattcg gtacgagacc ccgttggtgc tggtgatgcg caacaccact
    13921 cgcgacgtcg agttgcacgg catgacgatt ccagaaggcg ctcaagtcaa cctgtgcatg
    13981 ggttcggcca atcgcgacga caagcggtgg gagaaccccg acgtcttcga cattcagcga
    14041 ccccgtcgcg cgcacatctc gtttgcgggc gggattcaca gctgcctcgg aatgcacctc
    14101 gcgcgggtcg agaccaaagc catgctcacc agcctcttcg atcgtgtgac ggatct
//
LOCUS       DASYLX010000161         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3627632,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000161 DASYLX010000000
VERSION     DASYLX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627632"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGP27_03920"
     CDS             complement(<1..471)
                     /locus_tag="VGP27_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850498.1"
                     /translation="MLHGRASLRPNDIAFTFTDYEKDWAGVAESVTWSQLSRRTMNVA
                     RELGLHASVGDRALILAPQSLDYVAAFLGSMQAGLIAVPLPLPHRGASDERVGAVLAD
                     TSPSVVLTTSTAAEDVGDYVDQSRMDTAPKIVEIDSMNLDADGGPGIPIADFPST"
     gene            complement(552..1691)
                     /gene="pe"
                     /locus_tag="VGP27_03925"
     CDS             complement(552..1691)
                     /gene="pe"
                     /locus_tag="VGP27_03925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase PE"
                     /protein_id="HEV7850499.1"
                     /translation="MRRLVAVVTALVTVSATGCFGSAIAAADDAQAGWTPDRGAPTDG
                     PAPLGTPGRGYALGGAHVLGIPYDEYIKRTGADWFPGLDRQIVDYPAGQVQGHTLERL
                     FPGIGRLGDGFPGLGIDGPSIGESVDEGEGNLETAIRNGGPGTAIGLSEGAMVLDAVQ
                     AKLANDPNAPPPDKLSFATYGDPLARHAFGESFLTQMFPVGSVVPSLDFRMPAPVESQ
                     YDTYQFVSAYDSIADWPDRPDNWMSLANAVVGLATGHTAVAFTNPSNVPPQNIRTAVN
                     SKGAKTTTYMIPEEHLPLVLPFKYLGVDEGTLNKLDGVLKPMVDAGYSRNDDPLTAPI
                     TVDPVRGYDPAAVTAPATQAAFGGAADPVSQLLSGFQYVLNHSGG"
     gene            complement(1736..>4295)
                     /locus_tag="VGP27_03930"
     CDS             complement(1736..>4295)
                     /locus_tag="VGP27_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HEV7850500.1"
                     /translation="RVADIIKHNAVAGGPLTVHVTGPAATVADLTVAGEQDRMPIEIA
                     IGVLVLLVLLLVYRNPVTMLLPLAAIGMSLVTAQAVVAGVSELFGIGVSNQSVIFLSA
                     MIAGAGTDYAVFLISRYHDYLRQGADLDDAVRRALISIGKVITASAATVGVTFLGISF
                     ARMGVFSTIGVACAIGVGVAFLAAITLLPAILVLAGPRGWVNPRRELTTRFWRRSGVR
                     IVRRPKTHLVASLLVLIVLASCATLVRYNYDDRKAVGPSAESSVGYAALERHFPISQA
                     VPEYILVQSPRDLRTPQALADLEQMAQRISQLPNIGLISGITRPTGSVPPEFRATYQA
                     GIVGDRMAAGSAVINDNTKDLKRLTNGAGTLADSLGDVRAQINRIAPGMQRLIDAISS
                     MRSQLGGDKLVKDVQAAAQLVQSINALGNEMGVNFSTVRDMFAWVGPVLAALNGNAIC
                     DANPSCSATRIQFQKLVDARNDGRLDQINTLAGQLQGFGDKQTLNATVKQLNDTLSNF
                     SSAVRSMGLDRPGGLQAGLTQVRQGANRLADGSKQVAGGVDQLVGQVKLMGAGLDEAA
                     AFLLAMRNEAADPSMAGFNIPAQILQLDDFKKAAKIFISSDGHTARYLVQTTLNPFSS
                     EAMDQVNEISDTAQSAQPNTQLADAKISIGGFPASLRDTRNYYEHDIRFIIVVTLVVV
                     LLILMALLRAIVAPLYLIGSVIVSYFAALGISVLTFQYLLGQQLHWSVPPLAFVVLVA
                     VGADYNLLFVSRMRDESPHGMRYGVIRTLTSTGGVITAAGLIFAASMCGLLFSSITTV
                     VQGGFVIGVGVLLDTFLVRTVTVPAMATLVGRANWWPSRLSDQRSPPATAGQAG"
BASE COUNT          776 a         1371 c         1376 g          772 t
ORIGIN      
        1 cgtgctaggg aaatcagcta tcggaatacc cggtccacca tcggcgtcca gattcatcga
       61 atcgatctcg acgattttcg gagcggtgtc catccgcgat tgatcgacgt aatcgccgac
      121 gtcttccgcg gccgtagacg ttgtgagaac aaccgacggc gacgtatcgg ccaggactgc
      181 acctacccgc tcatcactcg cgccgcggtg cggcagcggg agcggaaccg cgatcagccc
      241 agcctgcatg gatcccagga atgccgcaac gtagtcaagg ctttgcggag caaggatcaa
      301 cgctctgtct ccgaccgacg catgaaggcc gagctcgcgt gccacattca ttgttcggcg
      361 ggataactgt gaccacgtga cactctcggc aacgcccgcc cagtcctttt cgtagtcggt
      421 gaacgtgaac gcaatgtcat tggggcgcag gctggctcgt ccgtgcagca tcgagagaat
      481 ggaagactga ggcgaaggcg tcaaatcacg tccgtagaaa ctagtcgaat taatcgggct
      541 ggaagtcgtt gtcagccgcc gctgtggttc agcacgtact ggaagccgga cagcaactgc
      601 gacacagggt cggccgcacc gccgaaggcg gcctgcgtgg ccggtgcggt gacggcggcc
      661 gggtcgtagc cgcgtaccgg atccaccgtg atcggagcgg tcagcggatc gtcgtttcgt
      721 gaatacccgg catccaccat cggcttcagc accccgtcaa gcttattcag cgtgccttca
      781 tccaccccga ggtatttgaa cggcagcacc agagggaggt gctcctccgg aatcatgtat
      841 gtcgtcgtct tcgcaccctt ggagttgacg gccgtcctga tgttctgcgg cggcacgtta
      901 ctcgggttgg tgaacgccac cgcggtgtgg ccggtcgcga gacccacgac cgcgttggcg
      961 agggacatcc aattgtccgg cctgtccggc cagtcggcga tgctgtcgta cgcagacacg
     1021 aactggtagg tgtcgtactg gctttccacc ggagccggca tgcggaagtc gagcgagggc
     1081 acgacgctgc cgacggggaa catctgggtc aggaagctct cgccgaaggc gtgcctggca
     1141 agcgggtcgc catacgtggc gaagctcaat ttatccggtg gcggcgcgtt cggatcgttt
     1201 gcgagcttcg cctgaacggc gtcgagcacc atcgctccct cggacaaccc gatcgcggtg
     1261 ccgggaccgc cgttgcggat cgccgtttcc aggttgccct cgccctcgtc gaccgactcg
     1321 ccgatgctgg ggccgtcgat gcccaggcca gggaagccgt cacccagccg gccgatgccc
     1381 gggaacaacc gttccagggt gtgcccctga acctgacccg cgggatagtc gacgatctgc
     1441 ctgtccagtc cggggaacca gtcggcgccg gtgcgcttta tgtactcgtc atagggaatg
     1501 ccaagaacgt gcgcaccgcc cagcgcgtaa cctcttccag gcgtccccag gggcgccggc
     1561 ccgtcggtcg gcgcgccgcg gtccggcgtc cagccggctt gtgcgtcatc ggctgccgcg
     1621 atcgcggacc cgaaacaccc tgtggcgctg acggttacca gcgcagtgac cactgcgacc
     1681 agtctccgca tccctgctcc cgcctgctgg ctacgcgtcc gtagcggtcc cgacattacc
     1741 ccgcctgccc cgctgttgcc ggcggcgacc gctggtcgct cagtcgtgag ggccaccagt
     1801 tcgcccgccc caccagcgtc gccatggcgg gcaccgtgac ggttcgcacc aggaaggtgt
     1861 ccagcaggac gccgacacca atgacgaatc cgccctgcac cacggtggtg atgctggaga
     1921 acagcagacc gcacatcgag gcggcgaaga tcaaaccagc tgcagtgatc accccgccgg
     1981 tcgaggtcag ggtgcgaatg acgccgtaac gcatgccgtg cggagattcg tcgcgcattc
     2041 gcgatacgaa gagcaggttg tagtcggcgc ccaccgcaac cagcaccacg aaggccaatg
     2101 gcggtacgct ccaatgcaat tgctggccga gtaggtattg aaacgtgagc acgctgatac
     2161 ccagtgccgc gaagtacgag acgattacgg agccgatcag atagagcggc gcgacgatcg
     2221 cacgcagcag cgccatcagg atgaggagaa cgacgaccag ggtcacgacg atgatgaaac
     2281 ggatgtcgtg ctcgtagtag ttgcgcgtgt cccgcagcga ggcgggaaat ccacctatcg
     2341 atatcttggc gtccgccagt tgggtgttcg gttgggcgct ttgcgcggtg tcactgatct
     2401 cgttgacctg atccatcgct tcggagctga acgggttcag tgtcgtttgc accaggtacc
     2461 gggccgtgtg gccatccgac gagatgaaga tcttcgcggc cttcttgaag tcgtcgagct
     2521 ggaggatctg ggccggaatg ttgaaccccg ccatcgacgg atctgccgcc tcattccgca
     2581 tcgccaacag aaacgcggcg gcctcatcga ggccggcgcc catcagcttg acctggccga
     2641 cgagttggtc aactccgcct gcgacctgct tgctgccgtc tgccagccga ttcgcgccct
     2701 gcctgacctg ggtcaggccc gcctgcaggc ctccgggtcg gtcgagaccc atcgagcgca
     2761 ccgccgagct gaagttcgag agcgtgtcgt tgagttgttt caccgttgcg ttgagggtct
     2821 gtttgtctcc gaacccctgt agttgcccgg ccagagtatt gatttggtcg aggcgtccgt
     2881 catttcgagc gtcgaccagt ttctgaaact gaatgcgggt ggcgctgcac gacggattgg
     2941 catcgcagat cgcgttgccg ttcagcgccg ccaacaccgg acccacccag gcgaacatgt
     3001 ccctgacggt ggagaagttc acgcccattt cgttgccgag cgcgttgatg ctctgcacca
     3061 gttgggcggc ggcttggacg tctttgacca acttgtcacc gcccagttgg cttcgcatcg
     3121 acgatatggc gtcgatcagg cgttgcatgc cgggagcgat ccgattgatc tgagcccgca
     3181 cgtcgccgag gctgtcggcc agcgtgcctg ccccgttcgt cagtcgcttg agatctttgg
     3241 tgttgtcgtt gatcacggcc gagcctgcgg ccatccgatc accgacaatg cccgcttgat
     3301 aggtggctct gaactccggc ggcacgcttc cggtgggccg ggtgatgccg ctgatgagac
     3361 cgatgttcgg cagttggctg atccgctgtg ccatctgctc caggtccgcg agggcctgcg
     3421 gcgtgcgcag gtcgcgcggc gactgaacga ggatgtattc gggcacggcc tgactgatgg
     3481 ggaaatgacg ttccagcgcg gcgtagccga ccgaactctc cgccgatggt ccaacggcct
     3541 tgcgatcgtc gtagttgtag cgcaccaggg ttgcacagct ggccagaacg atcagcacga
     3601 gcaggctggc aaccagatgg gtcttcggcc gccgcacgat gcgtaccccc gaacgtcgcc
     3661 agaaccgcgt ggtcagttcg cgccgcgggt tgacccagcc gcgcggcccg gccagcacca
     3721 ggatcgccgg cagcaacgtg atcgcggcaa ggaaggccac cccgaccccg atcgcgcacg
     3781 ccacaccgat cgtcgagaac acccccatcc gggcgaagct gatcccgagg aaggtgactc
     3841 ccacggtggc cgccgacgcg gtgatcacct tgccgatcga gatcaacgcc cgccggaccg
     3901 cgtcatcgag atctgcccct tgccgcagat agtcgtgata ccggctgatg agaaagaccg
     3961 cgtagtccgt tcccgcgccc gcgatcattg cgctcagaaa gatcacagac tggttcgaaa
     4021 cacctattcc gaagagttcc gagaccccgg ccaccacggc ctgtgccgtc accagggaca
     4081 tcccgatggc cgccaacggc agaagcatgg tgacggggtt gcggtacacc aatagaagaa
     4141 cgaggagtac caggacgccg atcgcaatct cgatgggcat tcggtcctgc tcgccggcga
     4201 cggtgaggtc ggcgacggtg gccgcaggcc cggtgacgtg cacggtgagc gggccgccgg
     4261 cgacggcgtt gtgcttgatg atgtcggcaa ctcgg
//
LOCUS       DASYLX010000162         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3188582,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000162 DASYLX010000000
VERSION     DASYLX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188582"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGP27_03935"
     CDS             complement(<1..891)
                     /locus_tag="VGP27_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850501.1"
                     /translation="MQLTFDSDVEEFRAEFSAFLDEHLPSEAETLERPRSVSHMPQWS
                     RDWQRLLFDNGWLLPAQPPEFGGRNATVLQSFVHLDELCRRRIYHSMNPQGVNIIAAS
                     LISFGSDEQKHRWAVPVLRGELTASLGMSEPSAGSDLASLRTRAVADGEHFVVNGQKV
                     WTSGAHDADFLLAFVRTDPDAPKHKGISALIIPTDTPGVVCRPFADMCAEDNLDFNEV
                     FFTDARVPAENLVGPLNGGWGVANGSLGHERTMMWLGFADRIDNMIADFRPRGELQRD
                     QYATTIMDYLALRAMGSAALA"
     gene            complement(875..1873)
                     /locus_tag="VGP27_03940"
     CDS             complement(875..1873)
                     /locus_tag="VGP27_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850502.1"
                     /translation="MLLEFDADQRLWQDTVRDAVGKQCPPSLVREVAENGVDPAPLWQ
                     AYIDAGWTELTDPENAVELAIVLEELGRATDPTPFLATLSQFAPLVGDRFDPQQAGAA
                     IYDGISAYRDADGWVLDGTARHVLDADRAQRLAVVTDGGVFLVDADKATVRRSKVFDP
                     VLHIADVSFAGVRLADTERLHTDPEKARHLALTGIAITTVGACQRIIDLALEHVKQRQ
                     QFGVAIGSFQAVQHKAVDMHVATERARALSYFAALTISADDPRRRLAAAMAKAAAGEC
                     QAVVFRHGLQLFGAMGFTWENDLQFALKRAKAGELLLGGAAEHRAMIAEEFDAADF"
     gene            2116..3216
                     /locus_tag="VGP27_03945"
     CDS             2116..3216
                     /locus_tag="VGP27_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850503.1"
                     /translation="MIQHPDGAASDKTPVIDASVHIFAQSNKDLRKNFMAEPYRSRGF
                     PDYEMDWYGAPGGEYAKGTEGPDGQYPGSDPEVVGRHLFDDRGVDVAILHPMTRGIMP
                     DRHLGTAIAAGHNEMLVTRWLDEGSYRDRFRGTIRVNPDDIAGALREIDKYKDHPRVV
                     QIGVPLQSREVYGKPQYWPLWEAAVDAGLPVAVHIEVGAGIQFAPTPSGVTRTYEQYV
                     SFMALNYLYHLMNMIAEGVFERMPDLKFVWADGAADLLTPFMWRMDCFGRPHLEQTPW
                     APKMPSDYLPGHVFFIQGAVDGPGDVDFAGEWSAFTGKDDMVMYGSSYPHWQANELTV
                     PSAYTAEQRDKLCWRNAAELYGIDVGAGVGAE"
     gene            3248..>3739
                     /locus_tag="VGP27_03950"
     CDS             3248..>3739
                     /locus_tag="VGP27_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7850504.1"
                     /translation="MTVTTTERRPAAERIAVRCVDSDVHPVPRSGELTQYIPEPWRSK
                     YFLTRKIGEQIYYDAPDYAHAYAMRADTFPPDGQFPGSSPDLAFKQLIMEAGADIAIL
                     EPAAYPARFREPNHAMSCALNDWQANHWLDSHNNWHERWRGSICAAVEEPDGAAREIE
                     RWAG"
BASE COUNT          663 a         1249 c         1234 g          593 t
ORIGIN      
        1 ggccagcgcc gctgatccca tcgcccgcaa ggccaggtag tccatgatcg tggtggcgta
       61 ctggtcgcgc tgtagctcgc cgcgcggccg gaagtcggcg atcatgttgt cgatccggtc
      121 ggcgaagccc agccacatca tcgtccgctc gtgtccgagt gagccgttgg ccacacccca
      181 ccctccgttg agtggtccga cgaggttctc ggcgggaacc ctggcgtcgg tgaagaacac
      241 ctcgttgaag tccagattgt cttcagcgca catatcggcg aacgggcggc agaccacccc
      301 cggcgtgtcg gtggggatga tcaaggcact gatccccttg tgcttgggag catccgggtc
      361 ggtgcgtacg aacgcgagca ggaagtcggc gtcatgggca ccggaggtcc acaccttctg
      421 gccattgacg acgaagtgct cgccgtcggc cacggcgcgg gtgcgcagcg acgccagatc
      481 ggagcccgca ctgggctcgc tcatccccag cgacgcggtg agctcacccc tgagcacggg
      541 taccgcccag cggtgcttct gctcgtcgga tccgaacgag atcagcgatg cggcaatgat
      601 attgacgccc tggggattca tgctgtggta gatgcggcgg cggcacagct cgtcgagatg
      661 gacgaacgac tgcagcaccg tcgcgttgcg gccaccgaac tccggcggct gggcgggcag
      721 caaccagccg ttgtcgaaca gcagccgctg ccagtcgcgc gaccactgcg gcatgtgcga
      781 caccgaccgc ggccgctcca gcgtctcggc ctccgacggg agatgctcgt cgaggaacgc
      841 cgagaactcg gcgcggaact cctcgacgtc ggaatcaaaa gtcagctgca tcgaattcct
      901 ccgcgatcat tgcgcgatgt tcggccgcgc cgccgagcag caactcgccc gccttggccc
      961 gcttcagggc gaactgaaga tcgttttccc acgtgaatcc catggcgccg aacagctgaa
     1021 ggccgtgccg gaacacgacg gcctggcact cccccgccgc ggccttggcc atggcagccg
     1081 cgagacgacg gcgcggatcg tcggccgaga tggtcagcgc agcgaagtac gacagtgcgc
     1141 gggcgcgctc ggtggccaca tgcatgtcga cggccttgtg ctgcacggcc tggaaggagc
     1201 cgatcgcgac gccgaactgc tggcgctgct tcacgtgctc gagcgcgagg tcgatgatgc
     1261 gctggcatgc gccgaccgtg gtgatggcga tcccggtgag cgccagatgg cgagccttct
     1321 ccggatcggt gtgcagccgt tcggtgtcgg ccagccggac gcccgcgaag gacacgtcgg
     1381 cgatgtggag caccggatcg aagaccttgc tgcgccggac cgtcgccttg tcggcgtcga
     1441 cgaggaagac accgccgtcg gtcaccacgg cgagccgttg cgcccggtcg gcgtcgagca
     1501 cgtggcgtgc cgttccgtcg agcacccagc catcggcatc gcggtaggcg ctgatgccgt
     1561 cgtagatcgc cgcaccggcc tgctgggggt cgaagcggtc gccgaccagc ggcgcgaact
     1621 ggctcaacgt cgccaagaac ggcgtcggat cggtggcgcg accgagctct tcgagcacga
     1681 tcgccagctc gacggcattc tccggatcgg tcagctcggt ccagcccgcg tcgatgtagg
     1741 cctgccacag cggcgccggg tccacgccgt tctccgcgac ctcacggacc agggacggcg
     1801 ggcactgctt ccccaccgcg tcgcggacgg tgtcctgcca cagccgctga tctgcatcga
     1861 actcgagtag catccggcgc ctcctggtgc gattgcccgt cgcggacgag aataacattc
     1921 tccttagatg ccgtaactgt tctcgattaa cgactacccc gtttcgctcc ggggctgccg
     1981 tgcacgtcac cagcgcgcac cctgacctgc ggagaagtaa gcgttacgct tgagaaccca
     2041 attcttgcca ttgaagaact tggctttaca ctggctgtat ggccggtccc gaccggcaga
     2101 catcggaggc catcagtgat ccaacatcct gacggggctg cgtcggataa gacgcccgtc
     2161 atcgacgcca gcgtgcacat cttcgcccag tcgaacaagg acctccgcaa gaacttcatg
     2221 gccgagccgt accgcagccg tggcttcccc gactacgaga tggactggta cggcgcaccc
     2281 ggcggcgagt atgcaaaggg caccgagggc ccagacggcc agtaccccgg ttcggacccg
     2341 gaggtcgtcg gacggcactt gttcgacgac cgcggtgtcg acgtcgcgat cctgcacccc
     2401 atgacgcgcg gcatcatgcc ggaccggcac ctcggaacgg ccatcgccgc gggccacaac
     2461 gaaatgctgg tgacgcgctg gctcgacgag ggttcctacc gcgaccggtt ccgcggcacc
     2521 atcagggtca atccggacga catcgccgga gcgctgcgcg agatcgacaa gtacaaggac
     2581 cacccccggg tagtgcagat cggcgtcccc ctgcaatcgc gtgaggtcta cgggaagccg
     2641 cagtactggc cgttgtggga ggcggcggtc gacgccggcc tgccggttgc cgtgcacatc
     2701 gaggtgggcg cgggaattca gttcgcgccg acgccgtcgg gtgtcacgag aacctacgag
     2761 cagtacgtca gcttcatggc gctgaactac ctgtaccacc tgatgaacat gatcgccgag
     2821 ggggtattcg aaaggatgcc cgatctgaaa ttcgtctggg cggacggcgc cgccgacctg
     2881 ctgacaccct tcatgtggcg gatggactgc ttcggacgcc cgcacctcga acagacgccg
     2941 tgggccccga agatgccgag cgactacctg cccggccatg tcttcttcat ccagggcgcc
     3001 gtggacggcc ccggcgacgt cgacttcgcc ggcgagtggt cggccttcac cggcaaggac
     3061 gacatggtga tgtacgggtc aagctacccg cactggcagg ccaacgagct gacggtgccg
     3121 agcgcgtata cggccgaaca gcgcgacaag ctgtgctggc gcaacgccgc agagctgtac
     3181 gggatagacg tcggtgccgg tgtcggcgca gagtgatagc tacaggcttc gatagaggga
     3241 gaccacgatg acggtgacaa ccacagagcg caggcccgcg gccgagcgca ttgccgttcg
     3301 gtgcgtcgat tccgacgtcc atccggtgcc ccgaagcggc gagctgacgc agtacatccc
     3361 cgaaccgtgg cgcagtaagt actttctgac ccgcaagatc ggcgagcaga tctactacga
     3421 cgctccggac tacgcccatg cctacgcgat gcgcgccgac acctttcccc ccgacggcca
     3481 gtttcccggc agcagtccgg atctggcgtt caagcagttg atcatggagg caggcgccga
     3541 catcgcgatc ctggagcctg ccgcctatcc ggcccgcttc cgcgagccga accacgccat
     3601 gagttgcgcg ctcaacgact ggcaggccaa ccactggctg gacagccaca acaactggca
     3661 cgagcggtgg cgcggctcca tctgcgcggc ggtcgaggag cccgacggcg cggcgcgtga
     3721 gatcgaacgc tgggccggg
//
LOCUS       DASYLX010000163         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_336271,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000163 DASYLX010000000
VERSION     DASYLX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336271"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1027
                     /locus_tag="VGP27_03955"
     CDS             2..1027
                     /locus_tag="VGP27_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV7850505.1"
                     /translation="MWARLPGFTVADLDAALYRRRSLVKHLAMRRTLWVVGVDDLPLI
                     QAAASDRVADNERRKLVADVQKAGIATDGERWLDRACEAVLAHLSMHGPTGARQLRAA
                     LPELAGTYDPAPGKRWGGDTPLSPRVLTVLSVRGDIVRGPNEGTWTSSRPQWAAATHW
                     LTESPGRISLEAARAELVRTWLRTFGPATVTDVKWWFGNTLTWARHALRDIDAVEVYL
                     DGTPGFVLPDDLDEAAAPDPWAALLPGLDVTVMGWYDRDWYLGEHRSQVFDTNGNAGP
                     TAWWNGRVVGGWTQDPAGRVELRLLEDVGRDARKALTRRAAELTDWLDGIRISPRFPS
                     PLSRARG"
     gene            complement(1010..1765)
                     /locus_tag="VGP27_03960"
     CDS             complement(1010..1765)
                     /locus_tag="VGP27_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850506.1"
                     /translation="MVIGASSGLGRCIGVGLAARGDQVALLARRRERIETAAKEAGQD
                     ALAVECDVTDEASVRSAIGAAAEGLGGIDNLVYTPAIGSVMRLVDTDAETWGRVFATN
                     VTGAAVATAAAVPYLTESAGKVVYLSSDAGTFGPPWPGLAAYGVSKAALERLIEAWRA
                     EHADIGFTNLIVGECAGGDGDSATGFSVGWDRELAGEFYPQWISRGCMPGQLMPVEDL
                     VEVVHTILRTSTATSMPVVVARGAPSPPSPARA"
     gene            complement(1834..>2559)
                     /locus_tag="VGP27_03965"
     CDS             complement(1834..>2559)
                     /locus_tag="VGP27_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEV7850507.1"
                     /translation="ATTEAKVRGYQPGRFSFNVKGGRCEACSGDGTIKIEMNFLPDVY
                     VPCEVCNGARYNRETLEVHYKGKTISEVLDMSIEDAAEFFKPISSIHRYLHTLVDVGL
                     GYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRTVYILDEPTTGLHFEDIRKLLKVLN
                     GLVDKGNTVIVIEHNLDVIKTSDWIIDLGPEGGSAGGTVVAEGTPEDVAAVPESYTGK
                     FLSEVLASMPAKPATRRRRKVSA"
BASE COUNT          396 a          876 c          863 g          424 t
ORIGIN      
        1 tctgtgggcg cgactgccgg ggttcaccgt ggccgacctc gacgccgcgc tctaccggcg
       61 acgcagtctg gtcaagcacc tcgctatgcg gcgcacgctg tgggtggtcg gcgtcgacga
      121 ccttccgttg atccaggccg cagccagcga tcgggtggcc gacaacgagc ggcggaaact
      181 ggtggccgat gtgcagaagg ctggcatcgc caccgatggt gagcgctggc tcgaccgcgc
      241 gtgtgaggcg gtgctggcac atctgagcat gcacggtccg accggtgcac gacagttgcg
      301 ggcggcgctg cccgagctcg cgggaaccta cgaccccgca cccgggaagc gttggggcgg
      361 cgacactccc ctgtctcctc gggtgctgac ggtgttgtcc gtacgcggcg atatcgtccg
      421 tggccccaac gagggcacct ggacgtcgtc gcgcccgcag tgggccgccg cgacgcactg
      481 gctcaccgaa tcacccggcc gcatctcgct ggaagcggcg cgcgctgagc tggttcgaac
      541 gtggctgcgc accttcgggc cggccaccgt gaccgacgtc aagtggtggt tcggcaacac
      601 gctgacatgg gcccgacacg cgctgcgcga tatcgacgcc gtcgaggtgt acctggacgg
      661 gacaccgggg ttcgtgctgc ccgacgacct cgacgaggcc gccgcccccg acccctgggc
      721 tgcgctgcta cccggcttgg atgtgaccgt catgggttgg tatgaccgcg actggtatct
      781 cggcgagcac cgcagtcaag tgttcgacac caacggcaat gccggtccga cggcctggtg
      841 gaacggccgc gtcgtcggcg gctggacgca ggacccggcc gggcgcgtcg agttgcggct
      901 gctggaagac gtcggccgcg acgcccgcaa ggcgctgaca cggcgggccg ccgagctgac
      961 cgactggctc gacgggattc ggatcagccc gcggttcccg tcgccgctgt cacgcgcgcg
     1021 cgggtgacgg tgggctgggc gccccccggg cgaccaccac cggcatcgat gtcgccgtgc
     1081 tggtgcgcag gatcgtgtgc accacctcga cgaggtcttc gaccggcatc agttggccgg
     1141 gcatgcagcc acgcgagatc cactgcgggt agaactcccc ggcaagctcg cggtcccagc
     1201 cgacgctgaa gcccgtcgcc gagtcgccgt caccacccgc gcattcgccg acgatgaggt
     1261 tggtgaaacc gatgtcggca tgctcggcgc gccacgcctc gatgagcctt tccagcgctg
     1321 ccttgctgac gccgtacgcg gcgagacccg gccacggagg gccgaacgtg ccggcgtccg
     1381 atgacagata aaccaccttg cccgccgatt ccgtgaggta cgggacggcc gctgccgtgg
     1441 cgacggcggc accggtgacg ttagtggcga acactcggcc ccatgtctcg gcgtcggtgt
     1501 cgacgagccg catgaccgag ccgatcgcag gcgtgtagac gaggttgtcg ataccgccga
     1561 gaccttccgc cgcggcgccg atggcagagc gcaccgacgc ctcgtcggtc acatcgcatt
     1621 cgactgcgag cgcgtcctgt ccggcctctt tggccgcagt ctcgatccgc tcacgacgac
     1681 gggccaacag ggcgacctga tcgccacgcg ccgccagtcc gactccaatg cagcgcccaa
     1741 gcccgctcga tgcgccgatc accaccgttt tcgacatgat ctcctcgccg ggccactacc
     1801 ggtaatggca ttctcggatg gggagaatat aacttacgcg ctgaccttgc ggcgacgccg
     1861 cgtcgcaggc ttggcgggca tcgaggcgag cacctccgag aggaacttgc cggtgtagct
     1921 ctccggcacg gccgcgacgt cctccggtgt gccctccgcg accacggtgc cgcctgccga
     1981 gccaccctca gggccgaggt cgatgatcca gtccgatgtc ttgatcacgt ccaggttgtg
     2041 ttcgatgacg atgaccgtgt tgcccttgtc gaccaggccg ttgagcactt tgagcagttt
     2101 gcggatgtct tcgaaatgca aacccgtggt cggctcgtcg aggatgtaga cggtgcgacc
     2161 ggtcgaccgc ttctgcagtt cggaggccag cttgacccgc tgcgcctcgc cgcccgacag
     2221 cgtcggagca ggctggccga gccgtacgta gccaagcccc acatcgacca gcgtgtgcag
     2281 atagcggtgg atcgaggaga tcggtttgaa gaactcggcc gcgtcctcga tcgacatgtc
     2341 tagcacctcg gagatggtct ttcccttgta gtgcacctcg agggtctccc ggttgtaccg
     2401 ggcgccgttg cacacctcgc acggcacgta cacatcgggc aggaagttca tctcgatctt
     2461 gatggtgccg tcgcctgagc acgcctcgca gcgaccgccc ttgacgttga acgagaaccg
     2521 gcccggctga tagccgcgca ccttcgcctc ggtggtggc
//
LOCUS       DASYLX010000164         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1982506,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000164 DASYLX010000000
VERSION     DASYLX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982506"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGP27_03970"
     CDS             complement(<1..761)
                     /locus_tag="VGP27_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV7850508.1"
                     /translation="MPNKVFVVGVGMTKFEKPGRREGWDYPDMARESGTKALEDAGIH
                     YSEVEQGFVGYCSGDSTSGNRALYELGMTGIPIVNVNNNCSTGSTALFLAAQTVRGGL
                     VDCAIALGFEKMASGPLQGGAEDRESPLGRHVKALADIDEFALPVAPWMFAAAGREHM
                     RKYGTTAEHFAKIGYKNHKHSVNNPYAQFQEEYTLDDILAARMISDPVTKLQCSPTSD
                     GSGAAIVASEAFVDKHGLAGQAVEIVGQAMTTDFD"
     gene            complement(946..>2338)
                     /locus_tag="VGP27_03975"
     CDS             complement(946..>2338)
                     /locus_tag="VGP27_03975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7850509.1"
                     /translation="LRKVLKNYGPMTPARAVAIVRQIASALDAAHESGIMHRDVKPEN
                     ILLTRDDFAYLVDFGIANAATDEKLTELGTAVGTYAYMAPERFTNDEVTYRADIYALA
                     CVLHECLTGAQPYPADSVSVVITSHLMQPIPRPSVMRPGIPAAFDQVIARGMAKRPAD
                     RYASAGDLAAAANDALTQRDQDQAANILQRSQEATMPGIAGAIGTALATPPGVTPSPY
                     QTPAPVPPQHTPPPPPTPPPFAAATPSAAHGQWNTPLPASTRQKSMTWIPLAAAGAVF
                     VLVLGAFGVWWVLKKDDSSGRSVATSTSTTRATTKTRAPSTTPPVNTANFDAKLLAVL
                     PASYDDGVCEPAHPPATGALATADCGQANVPTGPKVARYSIFADQTTLDQHFKDAVAQ
                     NTELVKCPGSDLDSPTAWNYNANPDEMAGQVACGTYDGNPDVTWTKDADLLLADAQGN
                     SLEELHQWWNDFG"
BASE COUNT          362 a          736 c          805 g          435 t
ORIGIN      
        1 ctgtcgaaat ctgtggtcat cgcctgcccg acgatctcca cggcctgacc cgccaaaccg
       61 tgcttgtcga cgaacgcctc gctggcgacg atcgctgcgc ctgacccgtc ggaggtgggt
      121 gagcactgca gcttggtcac cggatccgag atcatccggg cggccaagat gtcgtcgagg
      181 gtgtactcct cctgaaactg cgcatacggg ttgttgaccg aatgcttgtg gttcttgtag
      241 ccgatcttgg cgaaatgttc tgcggtggtg ccgtacttgc gcatgtgctc acggcccgcg
      301 gcggcgaaca tccacggtgc gaccggcaag gcgaactcgt cgatatcggc cagcgccttg
      361 acgtgacgac cgaggggtga ctcacggtct tccgcgccgc cctgcagcgg gccggacgcc
      421 attttctcga agccgagtgc gatcgcgcag tcaaccagtc cgccgcgaac cgtctgcgcc
      481 gcaaggaaaa gcgccgtcga acccgtcgaa cagttgttgt tcacgttgac gatcgggata
      541 ccggtcatcc cgagttcgta gagcgcccgg ttgccggacg tcgaatcgcc cgaacagtag
      601 ccgacgaagc cctgctcgac ctccgagtag tgaatgccgg cgtcctcgag tgctttcgtg
      661 cccgattcgc gcgccatatc cgggtagtcc cagccctcgc ggcggccggg cttctcgaac
      721 ttcgtcatcc cgacccccac gacgaaaacc ttgtttggca tctcctgcgt cccttcgctg
      781 tcctgcgatc gacagaaaca caaagcaagc gaactgtcta gatgccctat cgatgaggcc
      841 gcgcatgaga gaggtcaggg gagcagcgac gtgtgcccgg gcagtagttg aagacggact
      901 cgacgtcgaa gacggcggcg gccccgagcc gtcagcagcg attgctcagc cgaagtcgtt
      961 ccaccattgg tgcagctctt cgaggctgtt gccctgcgcg tcggccaaca gaaggtcggc
     1021 gtccttggtc cacgtgacgt cggggttgcc gtcgtaggta ccgcacgcga cctggcccgc
     1081 catctcgtcg gggttcgcgt tgtagttcca cgccgttggc gagtcgaggt cgctgccggg
     1141 acacttcacc agctcggtgt tctgcgcgac ggcgtccttg aaatgctggt ccagcgtcgt
     1201 ctggtccgcg aagatcgaat accgcgccac cttcggaccg gtgggcacat tggcttggcc
     1261 acagtccgcc gtggccaggg cgccggtggc cggaggatgg gcgggttcgc acacgccgtc
     1321 gtcgtagctg gcgggcaaca cggcgagcag cttggcgtcg aagttggcgg tgttcaccgg
     1381 cggcgtcgtg ctcggagccc tcgtcttggt ggtcgcacgc gtcgtcgagg tgctggtggc
     1441 gacgcttctg ccagacgagt cgtccttctt gaggacccac cacacgccga aggcgccgag
     1501 gaccagaacg aacacggcac cggcggcggc caacgggatc caggtcatgc tcttctgccg
     1561 ggttgaagcc ggcagcggag tgttccactg cccgtgcgcc gcggacgggg tggcggcggc
     1621 gaatggcggt ggagtgggcg gcggtggcgg tgtgtgctgc ggcggcaccg gcgcaggggt
     1681 ctgatacgga gacggtgtga cgccgggcgg ggtggccaac gcggtcccga tggcaccggc
     1741 gatgccgggc atcgtcgctt cctgactgcg ctgcaggatg ttcgcggcct gatcctgatc
     1801 acgttgtgtg agtgcgtcgt tggcggccgc cgcgagatcg cctgcgctgg catagcggtc
     1861 ggcgggcctc ttcgccatgc cgcgcgcgat cacctgatcg aaggcggccg gaatgccagg
     1921 ccgcatcacg ctcggccgcg gtatcggctg catcaggtgc gacgtgatca ccacgctgac
     1981 gctgtcggcg gggtaaggct gcgccccggt caggcattcg tgcagcacgc aagccagcgc
     2041 gtagatgtcc gcccggtagg tcacctcgtc attggtgaac cgctccggtg ccatgtaggc
     2101 gtatgtccct acggcagtgc ctaattcggt cagcttctcg tcggttgccg cattggcgat
     2161 gccgaagtcg accaggtacg cgaagtcgtc gcgggtcagc aggatgttct cgggcttgac
     2221 gtcgcggtgc atgatgccgg attcgtgggc ggcgtcgagc gccgacgcga tctggcgcac
     2281 gatcgccacg gcccgggcag gcgtcatcgg accgtagttc ttcaggacct tgcgcagg
//
LOCUS       DASYLX010000165         8327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2201674,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000165 DASYLX010000000
VERSION     DASYLX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201674"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="glgX"
                     /locus_tag="VGP27_03980"
     CDS             <1..1431
                     /gene="glgX"
                     /locus_tag="VGP27_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV7850510.1"
                     /translation="FGFFAPHYQYSFSRHAGGAVAEFKTMVRTFHEAGIEVILDVVYN
                     HTAEGSHLGPTINFRGIDNAAYYRLLDGDLKFYKDFTGTGNSLNARHPHTLQLIMDSL
                     RYWVLEMHVDGFRFDLAATLAREFYDVDRLSAFFDLVQQDPVVSQVKLIAEPWDIGEG
                     GYQVGNFPGLWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASI
                     NFVTCHDGFTLNDLVSYNEKHNEANGEDNRDGESHNRSWNCGVEGPTDDPDILALRGK
                     QMRNIMGTLMLSQGTPMLSHGDEMGRTQKGNNNVYCQDSELSWTDWSKCETNADHLAF
                     TRKVTQLRKSHSVFRRRRFFEGKPIRSGDQGRDIAWLTPAGNEMTPEDWGAGLAQCVA
                     VFLNGEAIPAPNERGERVVDDSFLLCFNAHDQEQDFIAPRGDYAKEWTADLDTANPTG
                     DTDLVVASGEKIPLQPRSLLVLRKTA"
     gene            1435..3720
                     /gene="treY"
                     /locus_tag="VGP27_03985"
     CDS             1435..3720
                     /gene="treY"
                     /locus_tag="VGP27_03985"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEV7850511.1"
                     /translation="MPSPVLSTYRLQMRGDCFTFADAEDLLPYLDELGVSHLYLSPIL
                     TAVHGSTHGYDVTDPTSVSAELGGAEGLGRLSAAARARGMGLIVDIVPNHVGVDDPKQ
                     NPWWWDVLEHGRDSPFSSFFDIDWTLDNGRIVLPVLGSDDDVDDVVVDGDVLRLGDLG
                     YPIAPGTGSGSGREVHDRQHYRLIGWRSGICGYRRFFSITSLAGLRQEDRAVFDATHV
                     EVKRWFDEGLVDGVRIDHPDGLSDPAGYLTWLREITGPQAWIVIEKILAVDEPLDPSL
                     PVAGTTGYDALREIGGLFLDPAGVGPLTDLVDSTGFSYDETSQIARALKVGAVTNTLG
                     SELSRLCRTSTAATAYVHPQLGEAVAVLLSRIGVYRSDYLNLSLVLPTALAEAVDDRP
                     DLAEPLAIVAAAVGSDRATAVRLQQLCGAATAKSMEDCLFYRDPRLVSLNEVGGEPDR
                     FGVSVAEFHQRATVRADMWPRAMTALSTHDTKRGEDVRARIGTLSQVPSLWAEFVSAW
                     EKRAPSPDPMTGLFLWQNIFGVWPADGAVSDELRGRLHAYAEKAIREADVHTTWNDPD
                     ADFEKAVHTWIDGLIDGPLATEVTKFVGRLDEHARSDSLGQKLIQLTAPGVPDVYQGT
                     ELWDDSLVDPDNRRPVDFSARREALSTMSHPKMRVVAAALRLRREKPDVFTDGGYTPL
                     LAEGPASAHLAAFVRGSDVLTAVTRHSVRLAESGWGETVFPLPTGTWKDRLGTGRYSG
                     SVSPADLFADLPVALLERVDD"
     gene            3713..5470
                     /gene="treZ"
                     /locus_tag="VGP27_03990"
     CDS             3713..5470
                     /gene="treZ"
                     /locus_tag="VGP27_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEV7850512.1"
                     /translation="MTDSATSARPTAFAVWAPRPHRVQLDVDGTVYPMTRSDDAWWRA
                     DVEARPNARYGFVLDDDPTVLPDPRSPRQPDGVHERSQLWQPASDAWSDDGWEGRSIE
                     GRAIYELHIGTFTPEGTFDSAIGRLDHLVDLGVDFVELMPVNAFGGTHGWGYDGVLWY
                     AVHEPYGGPDGLVRLVDACHNRGLGVLIDAVFNHLGPSGNYLPKFGPYLSTGSNPWGE
                     SINIADTDADEVRRYIIDCALRWMRDFHADGLRLDAVHALVDTTAIHILEELAAETEA
                     LAAELGRPLSLVAESDKNDPRLITPRDRGGYGMTAQWDDDIHHAIHAAVSGERQGYYG
                     DFGSIEALVATLKHGYFHAGTYSSFRRRRHGRPLDTATIPATRLLAYTLTHDQVGNRA
                     VGDRPSQNLSPGQQAVKAALALGSPYTAMLFMGEEWGSSSPFQFFSSHPEPDLARATA
                     EGRKREFAEHGWDADEIPDPQDPETFLRSKLNWDEIDDGDHSRLRRVYQGLLWLRRNE
                     PDMADHWLDDLKIDYDEDERWIVMHRGSLAFVCNLNSEDVTVPVTGEPLLAWGEPTVD
                     DDATRLDGHSFVVLRTVNS"
     gene            complement(5530..6813)
                     /gene="ilvA"
                     /locus_tag="VGP27_03995"
     CDS             complement(5530..6813)
                     /gene="ilvA"
                     /locus_tag="VGP27_03995"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV7850513.1"
                     /translation="MSTELSQNSRTSPPVSAADIDAAAQRISDVVTRTSLQLSERLSD
                     AVGATVYLKREDLQAVRSYKLRGATNLLRQLSAEEIAAGVVCSSAGNHAQGFAMACRS
                     MGIRGRVYVPAKTPKQKRDRIRYHGREFIELIAVGKTYDEAAEAALDDVARTGATLVP
                     PYDDPRTIAGQGTIAVELLDQLDDEPDLVIVPVGGGGCIAGITTYLAERTSRTSILGV
                     EPAGAAAMIAALAEGRPVTLDHVDQFVDGAAVNRAGALTYAALAAAGDMVSLTAVDEG
                     AVCTAMLDLYQNEGIIAEPAGALSVAALLEADITPESTVVCLISGGNNDISRYGEVLE
                     RSLVHLGLKHYFLVDFPQEPGALRRFLDTVLGPNDDITLFEYVKRNNRETGEALVGVE
                     LGSAADLEGLLERMKASDIHVETLEPGSPAYRYLL"
     gene            complement(6855..7292)
                     /locus_tag="VGP27_04000"
     CDS             complement(6855..7292)
                     /locus_tag="VGP27_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7850514.1"
                     /translation="MPLSGEYEPSTSDWARENAEQYMESGGTEGTELKGKPVILLTTI
                     GAKSGKVRKTPLMRVEHDGEYAVVASLGGAPKHPVWYHNVKKNPRVELQDGASTGDYD
                     AREVFGDEKAAWWERAVEAWPDYADYQKKTDRQIPVFVLTPVS"
     gene            7380..7607
                     /locus_tag="VGP27_04005"
     CDS             7380..7607
                     /locus_tag="VGP27_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850515.1"
                     /translation="MTTAEQFRASVNRATCEVSVAGVPWPAYKLYAVAVGVLVLVVGL
                     ATVSTATAVLSAAGAATIVWLAFSAYCSSRR"
     gene            complement(7604..8005)
                     /locus_tag="VGP27_04010"
     CDS             complement(7604..8005)
                     /locus_tag="VGP27_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850516.1"
                     /translation="MTSRHVSVWIDAAPEVAYAIAADPGQLPRWASGLAEGALRRTAD
                     GWVADSPMGEVIIEFAPINEYGVLDHVVRMPSGEAVYNPLRVIPAGEGQARCEVVFTV
                     RRRPGVTDEEFDSDVATVAADLDRLRQLAEG"
     gene            complement(8002..>8327)
                     /locus_tag="VGP27_04015"
     CDS             complement(8002..>8327)
                     /locus_tag="VGP27_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7850517.1"
                     /translation="AESAGPYAAQRGLADDSAEALAAHPDVISEIAAGVAAGNAKLAK
                     VERIKRFRVLPTFWEPGGDEITLTMKLKRKPIDEKYGSEIEELYASDIAEAVHQPKVT
                     TSAAQ"
BASE COUNT         1393 a         2808 c         2697 g         1429 t
ORIGIN      
        1 ttcgggttct tcgcgccgca ttatcagtac tcgttcagcc gacacgccgg cggagcggtc
       61 gccgagttca agaccatggt gcggacgttc cacgaggccg gcatcgaggt gatcctcgac
      121 gtggtctaca accacaccgc agagggcagt cacctcggcc cgacgatcaa cttccgcgga
      181 atcgacaacg ccgcctacta ccggctgctc gacggggatc tgaagttcta caaggacttc
      241 accggcaccg gcaacagcct caacgcccgc catccgcaca ctctgcagct gatcatggac
      301 tcgctgcgtt actgggtgct cgagatgcat gtggacggct ttcgtttcga tctcgccgcc
      361 acactggccc gcgagttcta cgacgttgac cggctgagcg cgtttttcga cctggtccag
      421 caggatcccg tggtcagcca ggtaaagctg atcgccgagc cgtgggacat cggcgagggc
      481 ggctaccagg tggggaattt tccaggtttg tggaccgagt ggaacgggaa atatcgcgac
      541 actgtgcgcg attattggcg gggcgaaccc gcgaccctcg gcgaattcgc ctcccggctg
      601 accggctcgt ctgacctcta tgaggcgacg gggcggcggc ccagcgcgag catcaacttc
      661 gtcacctgcc atgacggctt cacgttgaac gacctggtgt cgtacaacga gaaacacaac
      721 gaggccaacg gagaagacaa tcgcgacggc gagagccaca accggtcgtg gaactgcggc
      781 gtcgagggac cgaccgacga cccggacatc ctggcactac gcggtaagca gatgcgcaac
      841 atcatgggca cgctgatgtt gtcccagggc accccgatgc tgtcgcacgg tgacgagatg
      901 ggccgtaccc agaaaggcaa caacaacgtc tactgccaag actccgaact ttcctggacc
      961 gactggtcga agtgcgagac gaacgccgac catctggcat tcacccgcaa ggtgactcag
     1021 ttgcggaaaa gccattccgt gttccgccgt cgccggttct tcgagggcaa gccgatccgc
     1081 agtggcgacc agggccgcga catcgcgtgg ttgaccccgg ccggcaacga gatgacaccg
     1141 gaagactggg gtgcgggcct ggcccagtgt gtggcggtat tcctcaacgg cgaggccatc
     1201 ccggctccca acgaacgcgg cgagcgcgtc gtcgacgact cgttcctgtt gtgcttcaac
     1261 gcgcacgacc aggaacagga cttcatcgct ccgcgcggcg actacgccaa ggagtggacc
     1321 gccgacctgg acaccgccaa cccgacgggc gacaccgacc tcgtcgtcgc gtcgggcgag
     1381 aagatcccgc tgcagccccg gtcgctgttg gttctgcgta agaccgcctg agtcatgcct
     1441 tctcctgtgc tgtcgaccta tcgactccag atgcgcggcg actgctttac tttcgccgac
     1501 gccgaggacc tcttgcccta cctcgacgaa ctcggtgtct cgcacctcta cctgtccccg
     1561 atcctgaccg ctgtgcacgg ttcgacacac ggttacgacg tcaccgaccc cacctcggtg
     1621 tcggccgaac tgggcggcgc cgaagggctg ggccggttgt cggcggcggc gcgcgcgcgt
     1681 ggcatgggcc tgatcgtcga catcgtgccc aaccacgtcg gcgtcgatga tcccaagcag
     1741 aacccatggt ggtgggacgt gctcgagcac ggccgcgact cgccgttctc ctcctttttc
     1801 gatatcgact ggaccctgga caacggccga atcgtgttgc cggtgctggg ttctgatgac
     1861 gacgtcgatg acgtcgtggt agacggagat gtgctgcggc tcggcgacct cggctatccc
     1921 atcgcaccgg gaaccggctc gggcagcggc cgcgaagtgc acgaccgcca gcattaccgc
     1981 ctgatcggct ggcgcagcgg catatgcggt tatcggcggt tcttctcgat cacgtcactg
     2041 gccggtctgc ggcaggagga ccgtgcggtc ttcgacgcca cccacgtcga ggtgaagcgc
     2101 tggttcgacg agggccttgt cgacggcgtt cgcatcgacc acccggacgg attgtccgac
     2161 cccgccggct atctgacgtg gctgcgcgag atcaccggac cgcaggcctg gatcgtcatc
     2221 gaaaagatcc tcgccgtgga cgaacccctg gatccgagcc tgccggtggc gggcaccacc
     2281 ggctatgacg cgttgcgtga gatcggcggg ttgttcctcg atcccgcggg tgtcggcccg
     2341 ctgaccgacc tcgtcgattc gaccgggttc agctacgacg agacgtctca gatcgcgcgt
     2401 gcgctcaagg tcggcgccgt caccaacacc cttggaagcg agctgtcgcg gttgtgccga
     2461 accagcacgg ccgcaacggc ttacgtgcac ccgcagctgg gggaagccgt cgccgtgctg
     2521 ctgagccgta tcggtgtgta ccgctccgat tacctgaacc tgtcattggt gctgcccacc
     2581 gcgctggccg aggccgtcga cgatcgacct gatctggcgg agccgttggc gatcgtcgcc
     2641 gccgcggtcg gaagtgaccg cgcgacagcc gtgcgcctgc agcagttgtg cggcgccgcg
     2701 acggcgaagt cgatggagga ctgtctgttc tatcgcgacc cccgcctggt gtcgctcaac
     2761 gaggtcggcg gcgaaccgga ccgcttcggt gtcagcgtcg ccgaatttca ccagcgggcc
     2821 acggtgcgtg ccgacatgtg gccacgggcg atgacggcgt tgagcacaca cgacaccaag
     2881 cgcggcgaag acgtgcgcgc gcgaatcggg accctgtcac aggtgccgtc gctgtgggcg
     2941 gagttcgtga gtgcgtggga gaagcgcgcg ccctcccccg acccgatgac cggacttttt
     3001 ctgtggcaga acattttcgg cgtatggccg gccgacgggg cggtttccga tgaactgcgt
     3061 ggtcgcctgc acgcgtacgc cgagaaggcg atccgcgaag cggatgtcca cacaacctgg
     3121 aacgaccccg acgcggactt cgagaaagcc gtccacacct ggatcgacgg actcatcgat
     3181 gggccgctgg ccaccgaagt gaccaagttc gtgggacggc tcgacgagca tgcccgcagc
     3241 gacagcctcg gtcagaagct gatccagctg accgcacccg gcgtgcccga cgtctatcag
     3301 ggaaccgaat tgtgggacga cagcctggtc gatcccgata accgccgacc ggtcgacttc
     3361 tcggcgcggc gtgaggcgtt gtcgacgatg agccatccca agatgcgggt cgtcgccgcc
     3421 gccctccgac tccgccgcga gaagccggac gtcttcaccg acggtgggta cacgccgctg
     3481 ctggccgagg gcccggcgtc cgcgcacctg gcggcgttcg tccgggggtc agatgtgctg
     3541 accgcggtga ctcggcattc cgtgcggctc gccgaatcag gctggggcga aacggttttt
     3601 ccgctcccga ccgggacgtg gaaggaccgc ctcggcaccg gccggtacag cggatcggtg
     3661 tcacccgccg atctgttcgc cgatctgccg gtggcgctgc tggagcgggt cgatgactga
     3721 ttccgcaacg tcggctcggc cgacggcgtt cgccgtctgg gccccacggc cacaccgggt
     3781 gcagctggac gtcgacggca cggtgtaccc gatgacgcgg tccgacgacg catggtggcg
     3841 cgcggacgtc gaggcccgcc cgaacgcccg gtacgggttc gttctcgacg acgacccgac
     3901 ggtgctgccc gatccacgat cgccccggca gcccgacggc gtgcacgaac ggtcgcagct
     3961 gtggcaaccg gcatccgatg cgtggagcga cgacggctgg gaaggccggt cgatcgaggg
     4021 ccgcgcgata tacgaactac atatcggcac cttcactcca gagggcacgt tcgactcggc
     4081 catcggaagg ctggaccatc tggtcgatct cggtgtcgac ttcgtcgagc tcatgccggt
     4141 caatgcgttc ggcggtacgc acggctgggg atacgacggc gtcctctggt acgccgtgca
     4201 cgaaccgtac ggcggtcccg acggcctggt gcggctcgtc gacgcctgcc acaaccgcgg
     4261 tctcggggtg ctgatcgacg cggttttcaa ccatctcggt ccgtcgggca actaccttcc
     4321 gaagttcggg ccctatctgt cgacgggcag caacccgtgg ggcgaatcga tcaacatcgc
     4381 cgacaccgat gccgacgagg tgcggcgcta catcatcgat tgcgcgctgc gctggatgcg
     4441 cgactttcac gccgacggcc tgcggctcga cgccgtgcac gcactggtcg acaccacggc
     4501 gatccacata ctcgaggaac tggcagccga aaccgaggcg ctggcagcgg agttgggccg
     4561 accactgtcg ctggtcgcag agagcgacaa gaacgacccc cgactgatca caccccgtga
     4621 ccgcggcggc tatgggatga ccgcgcagtg ggacgatgac atccatcacg cgatccacgc
     4681 cgcggtgtcg ggtgaacgcc agggctacta cggcgacttc ggatcgatcg aggcgttggt
     4741 ggcgacgctg aagcacgggt acttccatgc gggcacctat tcgtcgttcc ggcgccggcg
     4801 ccacggtcgc ccactggata ccgcgacgat cccggccacc cggctgctgg cctacacact
     4861 cacccatgac caggtcggca accgagccgt cggcgaccgg ccctcgcaga acctgagtcc
     4921 cgggcagcag gccgtgaagg cagcgctcgc actcgggtcc ccttatacgg caatgctgtt
     4981 catgggtgag gagtggggat cgtcgagccc gttccagttc ttcagctctc atcccgaacc
     5041 ggatctggcc agggccaccg ccgagggtcg caaacgcgag ttcgccgaac acggttggga
     5101 tgcagacgaa atcccggacc cacaggatcc tgagacattc ctccggtcga agttgaattg
     5161 ggacgagatc gacgacgggg accacagccg gctgaggcgc gtctaccagg gactgctttg
     5221 gctgcggcgg aatgaacccg acatggccga ccactggctg gacgacttga agatcgacta
     5281 cgacgaggac gagcgctgga tcgtcatgca ccggggctcg cttgccttcg tctgcaacct
     5341 gaactccgag gacgtcacgg ttccggtgac cggcgagccg ctattggcat ggggcgagcc
     5401 gactgtcgac gacgacgcca cccggctgga cgggcattcc ttcgtagttc tgcggaccgt
     5461 caactcttga tccacagccg ccgtactcgg ggatgaccca tgagtaacga cgagcgcctc
     5521 gctggccgcc tagagcaggt aacggtacgc cggcgagccg ggctccaacg tctcgacatg
     5581 tatgtctgat gccttcatcc gctcgagcag accctcgagg tcggccgccg aaccgagttc
     5641 gactccgacc agcgcctcac ccgtctcgcg gttgttgcgc ttgacgtact cgaagagcgt
     5701 gatgtcgtcg ttcggtccga gcaccgtgtc gaggaaccgc cgcagtgcac ccggctcctg
     5761 cgggaagtcg acaaggaagt agtgcttgag cccgaggtgc accagcgagc gctccagcac
     5821 ctcgccgtac cgcgagatgt cgttgttccc accggagatc aggcagacca cagtcgattc
     5881 cggcgtaatg tcggcctcga gcaaagccgc caccgacagc gcgcccgcgg gctcggcgat
     5941 gatcccctcg ttctggtaca ggtcgagcat ggccgtgcac accgcgccct cgtccaccgc
     6001 cgtcagcgag accatgtcgc ccgcggcggc cagggccgcg taggtcaggg cgccggcgcg
     6061 gttgaccgcg gcgccgtcga cgaactggtc gacgtggtcg agggtgaccg gccgcccttc
     6121 ggccagcgcg gcgatcatcg ccgccgcccc cgcgggctcg acgcctagaa tcgaggtccg
     6181 gctggtgcgc tctgcaaggt atgtcgtgat gcctgcgatg cagccaccgc cgccgaccgg
     6241 aacgatcacc aagtccggct catcgtcgag ctgatcgagc agttccaccg cgatggtgcc
     6301 ctgcccggcg atcgtgcgcg gatcgtcgta cggcggcacc agcgtggccc cggtgcgcgc
     6361 cacgtcatcg agcgcagctt cggcagcctc gtcgtaagtt ttcccgacgg caatcaactc
     6421 gatgaactcg cggccgtggt aacggatgcg gtcgcgcttc tgcttgggcg tcttggcggg
     6481 tacgtacacc cggccgcgga tgcccatcga ccggcatgcc atcgcgaaac cctgcgcgtg
     6541 attgcccgcc gacgagcaca ccacccccgc ggcgatctcc tccgctgaca gctgccgcag
     6601 caggttggtg gccccgcgca gcttgtacga gcggaccgcc tgcagatctt cacgcttgag
     6661 gtacaccgtc gcgccgacgg catccgagag ccgttcgctg agctgtagcg aggtccgcgt
     6721 gaccacgtcg gaaattcgct gagcggccgc atcgatgtcg gccgcggaca ccggcggcga
     6781 cgtacgactg ttctggctca gttcagtgga caccgatcaa tggtgccatt tcggggtttt
     6841 cctgccaaaa cggattaact cacgggggtc aacacgaaga ccgggatctg gcggtcggtc
     6901 ttcttctgat agtcggcgta gtcgggccag gcctcgacag cgcgctccca ccaagccgcc
     6961 ttctcgtcgc cgaaaacctc acgggcgtcg tagtcgccgg tgctggcccc gtcctgcagt
     7021 tcgacgcgcg gattcttctt gacgttgtgg taccagaccg ggtgcttcgg cgcaccgccc
     7081 agcgatgcca ccacggcgta ctcgccgtca tgctcgacgc gcatcagcgg tgtcttgcgg
     7141 accttgccgc tcttggcccc gatggtggtc agcaggatga cgggcttgcc cttcagctcg
     7201 gtgccttctg tgccgccgga ctccatgtac tgctcggcgt tctcgcgggc ccaatcggac
     7261 gtgctgggtt cgtattctcc ggaaagtggc atagaagcag tctagatggg tagagacacg
     7321 ccaggccgga tcgaaagatt cggctggccg aaaaacaggt tgaggggtat cgtcgagcta
     7381 tgaccaccgc cgagcagttc cgcgcctcgg tcaaccgcgc gacatgcgag gtttcagtcg
     7441 cgggcgttcc gtggcccgcc tacaagctgt acgcggtcgc cgtgggcgtg ctggtgcttg
     7501 tcgtcggtct cgccacggtg agtaccgcga cggcggtctt gtccgccgcc ggcgccgcca
     7561 ccattgtttg gctggcgttc tccgcgtact gctcctcgcg ccgctaaccc tctgcgagct
     7621 gtcgcagccg gtcaaggtcg gcggccaccg tcgcgacatc gctgtcgaac tcctcgtcag
     7681 tcacgccggg acgccgtcgc accgtgaaca ccacttcgca acgcgcctga ccctcgcctg
     7741 ccggtatcac ccgcagcggg ttgtacaccg cctcgcccga cggcatccgg accacgtggt
     7801 caaggacccc gtactcgttg atcggcgcga actcgatgat cacctcgccc atcggcgaat
     7861 cggccaccca accatcggcg gtccgccgca gcgcgccctc cgccagtccg ctcgcccacc
     7921 ggggcagttg cccggggtcg gccgcgatgg cgtacgccac ctcgggggcg gcgtcgatcc
     7981 acacgctcac atggcgagac gtcactgcgc cgcgctcgtc gtcaccttcg gctggtgaac
     8041 cgcttcggcg atgtcggagg cgtacagttc ctcgatctcg gagccgtact tctcgtcgat
     8101 gggcttgcgc ttgagcttca tcgtcagcgt gatctcgtcg ccgccgggct cccagaacgt
     8161 cggcagcact cgaaagcgct tgatccgctc caccttcgcc agcttcgcgt tgccggctgc
     8221 gacgcctgca gcgatctcgg agatcacgtc ggggtgcgcg gccagagcct ccgccgaatc
     8281 gtcggccaat ccgcgttgcg ccgcatacgg cccagcggac tcggcgt
//
LOCUS       DASYLX010000166         6017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1544034,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000166 DASYLX010000000
VERSION     DASYLX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544034"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGP27_04020"
     CDS             complement(<1..672)
                     /locus_tag="VGP27_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7850518.1"
                     /translation="MTHESRQAAVDGHDHDTGHDHGSGHGHGGRGGKLGAALREVFAP
                     HRHDASDSIDGALESSAAGIRAVKISLLALGATSVAQLVIVMVSGSVALLADTVHNFS
                     DALTAIPLWIAFALGTKAATRRYTYGFGRAEDIAGLFVIAMITLSAIIAGFESIRRLI
                     NPVPIQHVGWVAAAGLVGFIGNELVAVYRIRVGRRIGSAALVADGLHARTDGFTSLAV
                     LFGAGG"
     gene            complement(669..1094)
                     /locus_tag="VGP27_04025"
     CDS             complement(669..1094)
                     /locus_tag="VGP27_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7850519.1"
                     /translation="MNADKGACGRRLPDDQVDLVVEVFRMLADATRVQVLWALVSREM
                     SVNDLATHVGKPAPSVSQHLAKLRMARLVRTRRVGTTIFYSLDNDHIGQLVTDAVFNA
                     EHAGPGVPGHHRDAAELAALHRDTINAELNAGRAKGRPA"
     gene            complement(1467..2909)
                     /locus_tag="VGP27_04030"
     CDS             complement(1467..2909)
                     /locus_tag="VGP27_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEV7850520.1"
                     /translation="MVNRYLQGPFAPLDREYTLTDLEVVGTIPAHLDGRYLRNGSNPV
                     GEIDPELYHWFIGDGMVHGIRIRDGRAEWYRNRWVRSAAAAKALGEPGPTGNRGLDPI
                     GANTNVIGHAGKTIALVEGGITNYELTDELDTVGICDFDGTLTGGYTAHPKHDPETGE
                     LHAVSYGFNRGNRVQYSVIGVDGRARRTVDITVGGSTMMHDFSLTEKHVVFYDLPVTF
                     DAPLAAEMTVPRGMRLATRLLLSALIGRVRIPDPLIARMPARKASDRRLPYAWNPHYP
                     ARVGVMPREGGDADVRWFDVEPCYVFHPMNAYDEGDTIVLDVVRNPKMFDRDRTGPNE
                     GPPTLDRWTVDLADGKVREVRFDDRAQEFPKVDPRLVGKRHRYGYTATVLEGIEGGEA
                     LLKHDLLKGRTDSRSFGSDKAVGEFVFHPSSPDASEDNGVVMGYVYDRPTDRSELAIL
                     DAQTLQDIARIELPHRVPAGFHGNWVPSDN"
     gene            complement(3009..3617)
                     /locus_tag="VGP27_04035"
     CDS             complement(3009..3617)
                     /locus_tag="VGP27_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV7850521.1"
                     /translation="MVIDTAAAVNGTQLGYARVSAAHQSLDQQTHALTAAGVDAARIY
                     SDKLSGTSTRQQRPGLDSLLDYARHGDAIVVVGIDRLGRNAAEVMQTIRHLGERGIVL
                     RSLREGIDTSNASGRMVAGVLASLAELELELGKERRTAARDARRARGQSIGRPKVLDD
                     SNTALARRLHASGESANTIAATLGVSRATVYRVIADESDSAV"
     gene            3840..4487
                     /locus_tag="VGP27_04040"
     CDS             3840..4487
                     /locus_tag="VGP27_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7850522.1"
                     /translation="MAGRGALMAGIAKQLGHPRGVGGRMVMLALNRGNRGFVRAAVQA
                     LHADDDAVVADVGFGGGVGLKLLLDSVGRSGSVHGVEVSDTMLKQAAKRYRRDVAAGR
                     LALHVGSLTRLPFADGTLNGVVTVNTIYFVAELQEAFNELARVTAHSGRIVVGVANPE
                     TLAKLPFTDDSFRLRPIPEVINTLQSAGFTVQHRRINAADGAPHLLIGTPAPNQA"
     gene            complement(5075..5148)
                     /locus_tag="VGP27_04045"
     tRNA            complement(5075..5148)
                     /locus_tag="VGP27_04045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5112..5114),aa:Pro,seq:cgg)
     gene            complement(5451..>6017)
                     /locus_tag="VGP27_04050"
     CDS             complement(5451..>6017)
                     /locus_tag="VGP27_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV7850523.1"
                     /translation="ESIYLQMGQEAHPIPEWIVVGAGTGGTSATIGRYIRYRRYATRM
                     CVVDPENSAFFPAYAQGRADVVTGMSSRIEGIGRPRVEPSFLPDVVDRMLTVPDAASI
                     AAAHHVSTVLGRRVGPSTGTNLWGAFHVLAEMVAEGRSGSVVTLIADSGDRYADTYFS
                     AEWLDSAGLDPSAAAGELVEFERSGRLR"
BASE COUNT         1084 a         1895 c         1924 g         1114 t
ORIGIN      
        1 gccgcctgct ccgaagagaa cggccagcga ggtgaatccg tctgtgcggg cgtgtaatcc
       61 gtcggcgaca agggcggctg agccgatacg acgcccgacg cggattcggt agacggcgac
      121 gagttcgttt ccgatgaacc ccactagacc ggcggcggcg acccagccga catgctgtat
      181 cgggaccggg ttgatcaggc gccggatcga ttcgaatccg gcgatgatgg ctgacagggt
      241 gatcatggcg atcacgaaca gtcccgcgat gtcttcggca cgaccgaacc cgtaggtgta
      301 gcgccgcgtc gcggccttgg tgccgagcgc aaacgcgatc cacaacggaa tagcagtcag
      361 ggcgtcggag aagttgtgca cggtgtcggc gagcagcgcg accgatcccg agaccatcac
      421 gatgaccaat tgagcgaccg acgtggcacc cagtgcgagc aagctgatct tcaccgcccg
      481 gattccggcg gcgctggact ccagggcacc gtcgatgctg tcggaggcgt cgtggcggtg
      541 cggggcgaat acttcccgca gcgcggcacc gagcttgccg ccccgtcccc cgtgtccgtg
      601 gccactgccg tggtcgtggc cagtgtcgtg gtcgtgtccg tcgacggcgg cctgacggct
      661 ctcgtgcgtc acgcgggtcg tcccttcgct cggcccgcgt tcagctcggc gttgatggtg
      721 tcgcggtgca gggccgcgag ttcggcggcg tcgcggtggt ggccgggcac tccgggtccg
      781 gcgtgttcgg cgttgaagac cgcgtcagtg accagttgcc cgatgtggtc gttgtccagg
      841 ctgtagaaga tggtggtgcc cacgcgacgt gtgcggacca accgcgccat ccgcagcttc
      901 gccaggtgct gggataccga cggcgcgggt ttcccgacat gagtggccag gtcgttgacc
      961 gacatctcac ggcttaccag cgcccacaac acctgcacgc gggtggcatc cgccagcatc
     1021 cgaaacactt cgaccaccag gtccacctgg tcgtccggaa gccgacgacc acaagctccc
     1081 ttatctgcat tcatacatag atagtaggcc ggtttggtcg agtggacagg actgccgcgg
     1141 taggcagaaa aaggaccctc ccgtattgcc cggtcgggcc ctggatctgc ttcgatgctg
     1201 tcggggtggc gggattcgaa cccacgacct cttcgtcccg aacgacacca ccagcgggtt
     1261 tggtgatgag gacggtgttt gaggggtttg aaattccaga tcaaaccgtt gattccgttt
     1321 gcgacccgtg cgcctcgacg aggctattgc ggactgactg cggactggtc tcacggcgat
     1381 tctgatcgga gagagcgggg gctggccgtg cgaggcgagg gttaagactg taggggtcac
     1441 gccccacccc ctgggcattc tcgttcttag ttgtcgctcg gcacccagtt gccgtggaat
     1501 ccggcgggca ctcggtgcgg caactcgatc ctcgcgatgt cttggagggt ttgagcatcg
     1561 aggattgcca actcgctgcg atctgttggc cggtcataga catagcccat caccacgcca
     1621 ttgtcctcac tcgcatcggg tgacgaagga tgaaacacga actcacccac agctttatcg
     1681 gagccgaagg agcgcgaatc ggtcctgccc ttgagtaggt cgtgcttgag cagagcttca
     1741 ccgccttcga taccttccag caccgtggcc gtgtacccgt accgatgccg tttgcccacc
     1801 aggcgagggt cgactttggg gaattcctgg gcgcgatcgt caaaccggac ttcgcgcact
     1861 ttcccgtcgg ccagatcgac cgtccaacgg tccaaggtgg gcggcccctc gttcggtccc
     1921 gtgcggtcgc ggtcaaacat cttggggttt cgtaccacgt cgagcactat cgtgtcgccc
     1981 tcgtcatagg cgttcatcgg atgaaatacg tagcacggct caacgtcgaa ccagcgcacg
     2041 tcagcatcgc caccctcgcg gggcatcacc cctacgcggg cggggtagtg ggggttccac
     2101 gcgtatggga gccgccggtc tgaggccttg cgtgctggca tgcgggcgat gaggggatct
     2161 gggatacgaa cccgcccgat caacgccgag agcagcagtc gggtcgccaa acgcattccg
     2221 cgcggcactg tcatctccgc cgctaacggg gcatcgaacg tgactggcag gtcatagaac
     2281 acgacgtgct tctcggtcaa cgaaaaatcg tgcatcattg tgctgccgcc caccgtgatg
     2341 tctacggtgc gtcgcgctcg accatcgacg ccgatcaccg agtactgcac cctgttcccc
     2401 cgattgaagc cgtaagagac cgcatgcagt tcgcctgttt ccgggtcatg ctttggatga
     2461 gcggtgtacc cgccggttaa cgttccgtcg aaatcgcaaa tcccgaccgt gtctaactca
     2521 tcggtgagtt cgtagttggt gatgccgccc tcaactaagg cgatggtctt accggcgtgt
     2581 ccgatcacat tcgtgttagc gccgattgga tctaagccac gattgccggt tggccccggc
     2641 tcgcccagtg ccttcgctgc ggcggcgctg cgcacccagc ggttgcgata ccactccgcc
     2701 ctgccgtcgc ggatgcggat gccatgcacc atgccgtcac cgatgaacca gtggtaaagc
     2761 tccggatcga tctccccgac gggattagac ccattgcgca gataccgccc gtccaggtgc
     2821 gccgggatgg tgccgacgac ctcaagatca gtaagcgtgt attcgcggtc gagcggggca
     2881 aacggaccct gcaagtagcg gttgaccatg gcgatctcct acgcggcgca aagccctccg
     2941 ttgcacctta tggatcacta tcgtgccgca aagccagtca atctgtacct ggttccgcgc
     3001 gcggcgaatt agacggcgga atcgctttcg tccgcaatca cccggtacac cgtcgcccgg
     3061 ctcacgccga gggtggcggc aatcgtgttc gcagactcgc cgctggcatg cagccgccgc
     3121 gccagcgccg tattggagtc gtcgaggacc ttcggccgac cgatggattg cccacgcgcc
     3181 cgtcgagcgt cccgcgctgc cgtgcggcgc tccttgccca gttccaattc aagctcggcc
     3241 aaactggcga gcactccggc caccatgcga cccgaagcgt tcgacgtgtc gatgccttcg
     3301 cgcagagagc gcaagacgat gccgcgctca cccaggtggc ggatggtctg cattacctca
     3361 gccgcgttgc gaccgaggcg gtcgatgccg accacgacga tggcgtcacc gtgccgtgcg
     3421 tagtccagca gagagtcaag gccggggcgc tgctggcggg tagacgtgcc cgacagcttg
     3481 tccgagtaga tgcgggcagc atcgacgcct gctgccgtca gcgcatgggt ctgctggtcg
     3541 agcgactgat gcgccgcgct cacgcgggcg tagccgagtt gggttccgtt gacggcggcc
     3601 gcggtgtcga tgaccatacg actatcgtct caaaagtctc agaacattca atatgagacg
     3661 taagttctga ggcatctttt gagactggat gagctggcag ttcctgacag cctaacgatc
     3721 tcgggcgtac tgcctcaaat ctttagaagc gacacagagg agtccggtga gcgggtgctt
     3781 gttcgtcggg tccgcgaatc gtgtgggtgt cgaacacgtt gagccggtac ggtcaggaga
     3841 tggccggccg gggcgctctc atggcaggta tcgccaagca gctcggccat ccacggggcg
     3901 tgggcggccg gatggtgatg ctcgcgctca atcgaggcaa ccgcggcttc gtgcgcgcag
     3961 ctgtgcaggc gttgcacgcc gacgacgatg ccgtggtggc cgatgtcgga tttggcggcg
     4021 gtgtgggcct gaagttgttg cttgacagcg ttggtcggtc ggggtccgtc catggagtgg
     4081 aagtgtcgga cacgatgctc aagcaggccg caaagcggta tcgacgcgac gtcgcggcgg
     4141 ggcggttggc gctgcacgtc gggtcgttga cccgactgcc gttcgccgat gggaccttga
     4201 acggtgtcgt taccgtcaac accatttact tcgtcgccga gctgcaggag gcgttcaacg
     4261 agttggccag ggtgacagca cactcgggtc gcatcgtcgt cggcgtcgcc aaccccgaga
     4321 cgttggcgaa gttaccgttc actgacgaca gcttccgcct tcgccccata cccgaggtca
     4381 tcaacacgct gcaaagcgcg ggtttcacgg tgcaacaccg tcggatcaat gccgcagacg
     4441 gggcacccca cctattgatc ggcacgccag caccgaacca ggcttaagat acgagcgtct
     4501 cgtttctcca caaacgagac gtcgtcgggg tccggtcatt agacgttcat gcgccaggca
     4561 gacgtttcga aaatatgttg ctcctcgggg cagcggcccg ggaccttctc cgagcagtcc
     4621 cgggccgccg gactgtcacg gaatgggtgg actcatttgc gcctactgcc agcgacaaaa
     4681 tttcgttgca gcctttgaga atccgagaac gagaacgtta gttctgagac ggagcgcgca
     4741 gaggtgctca gcttcgtcgc tctgagtgca accggtagcc cggtgagagc tgcccgccga
     4801 tcccgcacga tctacggcac actgctgcgt cgcttgcgat cagcggacgc gctaacctga
     4861 aggctgtgca gacgatgctg cgccatcagt cggcagtacc cactatggac acttacgccg
     4921 atctgttccc agatgactta gaactcgttt cagacgcgct agacaagggc cgacaggccg
     4981 ctctgcaagc tactgcggac caactgcgga ctgaggcaga aaaaagcccc gaccagacac
     5041 accggtcagg gtctcttaac tgcaacgacg cgagtcgggg tggcgggatt cgaacccacg
     5101 acctcttcgt cccgaacgaa gcgcgctacc aagctgcgcc acaccccgcg tgaagccacg
     5161 acagcgtatc gcaccggcca ggtgcgctgc caaatcgata tggcacggac cgatatgcgc
     5221 gaagtggtgg gtgcaaggac tcgatcgagc gacgcatgtc acgccacgcc gcgggatgga
     5281 acttgattgt cggtgtcgtt atgcacaatg acggcaggaa gccgcacatc ggtttaaggc
     5341 aggcgcgaga tgactggatt gggagcgtcc atttacgtgg gtttcttcgt cctggctgcg
     5401 ctgtggctgt ttctcacatc cgacgggccc ctcacccggg accccgacga tcagcgcaag
     5461 cggccggacc gctcgaactc cacgagctca ccggcggcgg ccgacgggtc aagcccggcg
     5521 ctgtccagcc attcggcact gaaataggtg tcggcgtagc gatcgccact gtcggcgatc
     5581 agcgtcacga ccgacccgct gcgcccttcg gcgaccatct ccgccagcac gtgaaacgct
     5641 ccccataggt tggtgccggt cgacggcccc acccgacggc ccagcaccgt gctgacgtga
     5701 tgggcggccg cgatcgacgc ggcgtcgggc accgtcagca tccggtcgac gacatcaggc
     5761 aggaatgacg gctcgacccg tggcctgccg atcccctcga tccgtgacga catcccggtg
     5821 acgacgtcgg cgcgcccctg cgcataggcg ggaaaaaacg ccgagttctc cgggtccacc
     5881 acgcacatcc gagtggcgta gcgccggtat cggatgtagc gaccgatggt cgcgctggtc
     5941 cctccggtgc ccgcgccgac gacgatccac tccggtatcg gatgggcctc ctgccccatc
     6001 tgcaggtaga tggactc
//
LOCUS       DASYLX010000167         7629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2092750,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000167 DASYLX010000000
VERSION     DASYLX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092750"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="mftF"
                     /locus_tag="VGP27_04055"
     CDS             complement(<1..859)
                     /gene="mftF"
                     /locus_tag="VGP27_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215210.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HEV7850524.1"
                     /translation="MIQPRLPDGFAVQVDRRVKVLGEGSALLGGSPTRLLRLAPAAQT
                     MLNGGRLEVHDALSAQVARTLLDATVAHPRPASGPSHRDVTVVIPVRDNPSGVSRLVA
                     ALRGMRVVVVDDGSVSPIKQTDFELMHCDVQVLRHPRSKGPASARNTGLTVCSTDFVA
                     FLDSDVVPRRGWLEALLGHFCDPAVALVAPRIVNLAQPDSLVARYEAVRSSLDLGQRE
                     APVVPYGTVSYVPSAAIICRRSVLKEVGGFDETLKSGEDVDLCWRFVEAGARLRYEPI
                     ATVAHEHRTQ"
     gene            complement(856..1602)
                     /gene="mftE"
                     /locus_tag="VGP27_04060"
     CDS             complement(856..1602)
                     /gene="mftE"
                     /locus_tag="VGP27_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HEV7850525.1"
                     /translation="MNSSYHRRVAFPSELGNSTSRQLRGTSPALVIPIGSTEQHGAHL
                     PLDTDTRIAAAVARGVAERLTEADHDSGWMLAPPIGYGASGEHESFPGTVSIGNAALR
                     LMLVEFGRSAANWARRQVFVNGHGGNVEALAAAVALLRYEGRDVGWCSCGVENADAHA
                     GHTETSVLLHISPDVVRVEEMAPGNRAPLAELLPAMRAGGMAAVSELGVLGDPTTATK
                     VDGERLLAEMIDGCARRIARWKPDRDGMLT"
     gene            complement(1740..2933)
                     /gene="mftD"
                     /locus_tag="VGP27_04065"
     CDS             complement(1740..2933)
                     /gene="mftD"
                     /locus_tag="VGP27_04065"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830272.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HEV7850526.1"
                     /translation="MARDKWFETVAFAQQRAKKRLPKSVYSALLAASEKGITVADNVE
                     AFGELGFAPHVIGAIEKRDLSTTVMGQDISLPVLISPTGVQAVHPDGEIAVARAAAAR
                     GTAMGLSSFASKPIEDVIAANPKTFFQVYWLGGRDAIAARVERARAAGAVGLIATTDW
                     SFAHGRDWGSPKIPEHMDLRTTVRMLPEGLTRPRWLMQWAKTMRPPDLRVPNQARLGE
                     PGPPFFAAYGEWMATPPPTWEDIAWLRELWGGPFMLKGVMRVDDAKRAVDAGVSAISV
                     SNHGGNNLDGTPASIRALTPIAEAVGDQIDVLLDGGVRRGSDVVKALALGARAVMIGR
                     AYLWGLAAEGQAGVENVLDVLRGGIDSVMMGLGRASIHDLVAGDVVVPPGFVRALGLP
                     DEAGV"
     gene            complement(2936..4129)
                     /gene="mftC"
                     /locus_tag="VGP27_04070"
     CDS             complement(2936..4129)
                     /gene="mftC"
                     /locus_tag="VGP27_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727659.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HEV7850527.1"
                     /translation="MTSAKSPEVQPVPRLVEQFERGLDAPICLTWELTYACNLACVHC
                     LSSSGKRDPRELSTQQCKDIIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVG
                     VKFSTNGVRITPEVATRLAASDYVDVQISLDGATAEVNDAVRGEGSFAMAVRALENLA
                     AAGFEDAKISVVVTRQNVDQLDEFAALAARYGATLRITRLRPSGRGADVWDELHPTPE
                     QQVQLYDWLVERGDRVLTGDSFFHLSGLGAPGALAGLNLCGAGRVVCLIDPVGDVYAC
                     PFAIHDRFLAGNILSDSGFDNVWKNAPLFRELREPQSAGACGSCGHYDSCRGGCMAAK
                     FFTGLPLDGPDPECVEGYGVPALERERVKPRPSGDHSRGTKQGPIPLKLLTSPPSRLC
                     NESPV"
     gene            complement(4126..4404)
                     /gene="mftB"
                     /locus_tag="VGP27_04075"
     CDS             complement(4126..4404)
                     /gene="mftB"
                     /locus_tag="VGP27_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892808.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HEV7850528.1"
                     /translation="MVTFDPNASWRLHHQVAVRPEPFGALLYHFGTRKLSFLKNRTIV
                     EVINSLGDHPDVRSACRAAGVDDAHQGPYLHALGVLAESKMLIAGENP"
     gene            complement(4430..4525)
                     /gene="mftA"
                     /locus_tag="VGP27_04080"
     CDS             complement(4430..4525)
                     /gene="mftA"
                     /locus_tag="VGP27_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101386.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HEV7850529.1"
                     /translation="MEPNQQAETDTELVTETLVEEVSIDGMCGVY"
     gene            4618..5217
                     /gene="mftR"
                     /locus_tag="VGP27_04085"
     CDS             4618..5217
                     /gene="mftR"
                     /locus_tag="VGP27_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209162.1"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from
                     the terminal Val-Tyr dipeptide of the precursor peptide
                     MftA, through a radical SAM enzyme-mediated process;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="HEV7850530.1"
                     /translation="MRPNSEHRVGRRRSTTWDHLSNVAIDLFAARGFDAVSVDDVAHA
                     AGIARRTLFRYYPSKNALPWGDFDAHLERMRDQLAGLDPGVPIGDALRTALLAFNTFD
                     DTETARHRQRMRVILQTAELQAYSMTMYAGWRAVVSAFVARRLGVTAGALVPQTVAWT
                     MLGAALSAYEHWLADESVSLAQALNSAFDTVSDGLRALA"
     gene            5285..5809
                     /locus_tag="VGP27_04090"
     CDS             5285..5809
                     /locus_tag="VGP27_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7850531.1"
                     /translation="MSAEREPDDAPRALLALYDEALPTVYGYFVRRCGDRGAAEDLTS
                     ETFLAAMDAARKDAPPTITVPWLIGVARHKLADHYRRRADRFTVPVAEPPEPVDAADG
                     WDAELDRIVAESVLAKLPEHHRTVLALRYMDDCSVPQCAELLGRTVHATDALLVRARR
                     AFRSHYPGPEGGTS"
     gene            5806..7125
                     /locus_tag="VGP27_04095"
     CDS             5806..7125
                     /locus_tag="VGP27_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7850532.1"
                     /translation="MNTEDARSGKDNSHDPLTALHGGELPVQPDPAFAARLRTRLESV
                     LSLPNRTGEVDMSGTDTAIAELNDPTSAPDVATAPPRAAALPYLSVANAREAIAWYTE
                     TFGASLVGEMYEMDDGRVGHAELEIGDGVIYLADEYPEIGLKAPAPQANSVSLMLPVA
                     DTDATLTRARELGAAVQREPYENYGARSATIIDPFGHRWMLSGPVTGAPIQIQHGDVG
                     YVSVWTPDSDRAAAFYGHVLGWTFDAETNQVTNTRERIGIHSVDAPPTLFCCYAVADL
                     DGARQAIVDGGGTVDRIEQFDFGTLRGATDSHGASFAVFQPNPGQPRPELNGSGPGEL
                     SYITYEVVDSAAFKAFYSRVLFWTFEPGRIADGWQVQQTHPMAGVAGGNAHQVTVPMW
                     TVDDIDAAVARVREAGGTVIDEPSQQPYGKSAQCTDDQGSRFYLGEH"
     gene            complement(7140..>7629)
                     /locus_tag="VGP27_04100"
     CDS             complement(7140..>7629)
                     /locus_tag="VGP27_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7850533.1"
                     /translation="ADLVVVGIGSKPAIDWLEGSGIDLDNGVVCDDVGRTATAHVWAI
                     GDVASWRHPVGHQVRVEHWSNVADQARVLVPAMLGKEIPATVSVPYFWSDQYDVKIQC
                     LGEPQADDTVHVVEDDGRKFLAFYERDGVVAGVVGGGMPGKVMKVRSKIASGAPIADV
                     LG"
BASE COUNT         1340 a         2570 c         2448 g         1271 t
ORIGIN      
        1 gttgcgtacg gtgctcgtgg gcgaccgtcg cgatcggctc gtagcgcagc cgagcaccgg
       61 cttcgacgaa ccgccagcac aggtcgacgt cctcccccga cttcagcgtc tcgtcgaatc
      121 cgccgacctc cttcagtacg gagcggcggc agatgatcgc cgcactcggc acgtatgaga
      181 cggtgccgta gggcacgacg ggtgcctcgc gctgcccgag gtccagtgag gagcgcaccg
      241 cctcgtaacg ggccaccagg ctgtccggtt gtgccagatt cacgatacgt ggtgccacca
      301 aagccactgc gggatcgcag aagtgtccga gcagcgcctc cagccagccg cgtcgcggca
      361 ccacgtcgga atcgaggaag gcaacgaagt ccgtgctgca aaccgtcaga ccggtgttgc
      421 gggcagatgc gggtcccttg ctacggggat ggcgcagcac ctgcacgtca cagtgcatga
      481 gctcgaagtc agtctgtttg atcgggctga ccgagccgtc gtcgacaacc accacacgca
      541 taccgcggag tgcggcaacc aatcgactta caccagaagg gttgtcgcgc accggaataa
      601 cgacagtgac gtcacggtgg gagggaccac tggcaggtcg agggtgggcg acggtggcgt
      661 cgagcagagt gcgcgcgacc tgggcgctga gcgcatcgtg cacctcgagc ctgccgccgt
      721 tgagcattgt ctgggcggcg ggtgcgagcc gcagcaggcg ggtgggcgat ccgcctagca
      781 gagccgaccc ctcgccgagc accttgactc ggcgatcaac ctgcacggcg aagccgtccg
      841 ggagtcgggg ctgaatcatg tcagcattcc gtcgcggtcg ggtttccagc gcgcgatccg
      901 acgcgcacac ccgtcgatca tctcggcaag gaggcgttca ccgtcgacct tggtggctgt
      961 cgtcgggtcg cccagtacgc ccaattcact cacggctgcc atcccgccgg cgcgcatcgc
     1021 gggcagcagt tccgccagcg gtgcccggtt ccccggcgcc atttcctcga cccgcaccac
     1081 gtccggcgaa atatgtagca atacagacgt ttcggtgtgt ccggcatgtg cgtcggcgtt
     1141 ctccacacca cacgagcacc acccgacatc ccggccctcg tacctcagca atgcgaccgc
     1201 ggcggctagg gcctccacat ttcccccgtg gccattgacg aacacctgcc gccttgccca
     1261 gttcgcggcc gaccggccga actccaccag catcaaccgt agcgcagcgt tgccgatcga
     1321 aaccgtgccg ggaaagctct cgtgttcgcc actggcgccg tagccgatcg gcggtgcaag
     1381 catccatcca gagtcatgat cggcctccgt cagccgttca gcaaccccac gagcgacagc
     1441 ggcggcgatc ctggtgtcgg tatccagcgg caaatgagcg ccatgctgtt cggtggaacc
     1501 tatcgggata accaacgcgg gtgatgtgcc tcgcagctgt ctcgacgttg agttcccaag
     1561 ctcgctgggg aaagccaccc gccgatggta ggacgaattc acctgccgtg cgccggttgt
     1621 tggcgcatgc gcatattttg ttttccacga atttgccgcg gaggctgtcg gctaaccacg
     1681 tctgtcccgc ctgccacgca tgtttcacgc gcaatctagc tggtagcgga gttgacgcgt
     1741 cagacgccgg cctcatccgg cagaccaagc gcccggacga agccaggagg aaccactacg
     1801 tcgccggcga cgaggtcgtg gatcgacgcc cggccgaggc ccatcatcac tgagtcgata
     1861 ccgccgcgca ggacgtcgag cacgttctcg acaccggcct ggccctcggc cgccaaaccc
     1921 cagagatacg cccgaccgat catgacagcc cgcgcgccga gcgccagtgc cttgaccacg
     1981 tcgctgccgc gacgcacgcc accgtcgagc agaacgtcga tctggtctcc tacggcttcg
     2041 gcgatggggg tcagcgcgcg aatggatgcg ggtgtcccgt ccaggttgtt gccaccgtgg
     2101 ttcgataccg agatcgccga aacaccggca tccacagcac gtttcgcgtc gtcgacccgc
     2161 atcacgccct tgagcatgaa cggtccgccc cagagttcgc gaagccaggc gatgtcctcc
     2221 caggtgggcg gtggcgtcgc catccactcg ccatacgctg cgaagaacgg cggaccgggc
     2281 tcgcccagcc gggcctgatt gggcacccga agatccggcg ggcgcatcgt cttggcccat
     2341 tgcatgagcc accgcggccg ggtgaggccc tcggggagca tccgcaccgt cgtgcgcagg
     2401 tccatgtgct cgggaatctt cgggctgccc cagtcacggc cgtgcgcgaa gctccaatcc
     2461 gttgtggcga tcaggccgac ggcgccagcg gctcgggcgc gttccacacg tgccgcgatg
     2521 gcatcccgtc cgcccagcca gtacacctgg aagaacgtct tggggttggc ggcgatgacg
     2581 tcctcgatcg gcttgcttgc aaacgacgac aatcccatcg ccgtgcccct cgcggcggcc
     2641 gcacgggcca cggcgatctc accgtctggg tgcaccgcct gcacccccgt tggcgatatc
     2701 agcacgggca gcgaaatatc ctgccccata acggtcgtcg acagatcgcg cttctcgatg
     2761 gcaccgatga cgtgcggggc gaacccgagc tcgccgaacg cctcgacgtt gtctgcgacg
     2821 gtgatgccct tctcgctggc ggcaagcaac gcgctgtaga ccgacttggg cagccgcttc
     2881 ttcgcgcgct gttgggcgaa tgccaccgtt tcgaaccact tgtctcgggc cacggtcaga
     2941 ccggactctc gttacacagc cgggaaggag ggctggtcag cagcttgagt gggatcggcc
     3001 cttgttttgt cccgcgggag tggtcaccgc tcggtcttgg cttgaccctc tcccgctcga
     3061 gcgccggaac gccatatccc tcaacacatt ctgggtcagg accgtcaaga ggcaggccgg
     3121 tgaagaactt tgccgccatg cagcctcccc ggcagctgtc atagtggcca cagctgccgc
     3181 acgcgcccgc cgactgaggc tcccgcagct cacgaaacag cggggcgttc ttccagacgt
     3241 tgtcgaaacc cgagtcggac agaatgtttc cggccaggaa tcggtcgtgg atggcgaatg
     3301 gacaggcgta gacgtcaccg accggatcta tcaaacagac cacccggccc gctccgcaga
     3361 ggttcagtcc ggcgagcgca cccggggcgc ccaggccgga caagtgaaag aaggagtcac
     3421 ccgtcagcac gcggtcgccg cgctcgacga gccagtcgta cagctgcacc tgctgctcgg
     3481 gggttgggtg cagttcatcc catacatcgg cgccgcggcc cgacgggcga aggcgggtga
     3541 tgcgaagggt tgcgccgtaa cgtgctgcca aagccgcgaa ttcatcgagc tgatcgacat
     3601 tctggcgagt gacaacaaca gagatcttgg cgtcttcaaa cccggcggcg gccagattct
     3661 ccagcgctcg cacggccatc gcgaaggagc cctcgccgcg cacggcatcg ttgacctctg
     3721 ccgtggcccc gtcgagggag atctgcacgt cgacgtagtc gctggcagcc agccgggtcg
     3781 ccacctcggg cgtgatccgc acaccgttgg tggaaaactt caccccgaca tggtgggcgg
     3841 tggcatagtc gaccagttcc cagaagtccg ggcgcacggt gggttcaccg ccgccgatgt
     3901 tgacgtagaa cacctgcatg cgttcgagct catcgatgat gtccttgcac tgctgcgtcg
     3961 acaactcgcg tggatcgcgt ttgcccgacg aggacaggca gtgcacgcac gccaggttgc
     4021 aggcgtacgt cagctcccag gtcaggcaga tcggcgcgtc gagccctcgc tcgaactgct
     4081 cgaccagccg tggcaccggt tgtacttccg gcgatttggc agacgtcatg ggttctctcc
     4141 cgctatgagc atcttcgact cggccagcac gcccagcgcg tgcaagtacg gaccctgatg
     4201 cgcgtcgtcg actcccgccg cgcggcaggc tgacctgacg tcggggtggt cccccagtga
     4261 gttgatcacc tcgacgatgg tccggttttt caggaacgac agcttgcgcg tgccgaagtg
     4321 gtacagcagt gcgccgaacg gttcagggcg caccgccacc tggtggtgca gccgccaact
     4381 ggcgttcgga tcgaacgtca cgataggcct gcttgctcac gcgtgcgggt cagtagaccc
     4441 cgcacatccc atcgatcgac acctcttcga ccagcgtctc ggtgacgagt tcggtgtcag
     4501 tttccgcctg ctgattcggc tccatgcgcc acgcctttct ctaccgggtc tcgacaaatg
     4561 tgatcgagat cgcaagaata tggcatcgag tgccagaatg gaagagcggg gttggtgatg
     4621 cggccgaatt ccgaacaccg ggtggggcgg cgtcgctcga cgacctggga ccacctcagc
     4681 aacgtcgcga tcgatctctt cgccgcacgc ggcttcgacg ccgtcagcgt cgacgacgtc
     4741 gcacacgccg caggaatcgc acgccgcacg ctcttccgct actacccgtc caagaacgca
     4801 ctgccatggg gcgatttcga cgcgcacctc gagcgcatgc gcgaccaact ggccggcctc
     4861 gacccgggcg tgccgatcgg tgatgcgctg cgaacggcgc tgttggcgtt caacaccttt
     4921 gacgacacgg agaccgcgcg gcaccggcag cggatgcggg tcattctgca gacagccgag
     4981 ctgcaggcct attcgatgac catgtatgcc ggatggcggg cggtggtctc cgcgttcgtc
     5041 gcgcgacggc tgggcgtcac ggcaggcgct ctggtgccgc agacagtggc atggacgatg
     5101 ctcggggcgg cgttgtccgc ctacgaacac tggctggctg acgagtcggt gtccttggca
     5161 caggcgctga acagtgcgtt cgacacggtc agcgacggtc ttcgagcgtt ggcctgagac
     5221 ggcgtcaaag aaattttttc gattggggcg tcggagttcg gcgcttcgag gcgacgataa
     5281 gggtgtgagc gccgaacgag agcctgatga cgctccgcgg gcgctgttgg cgctgtacga
     5341 cgaggcgctg cccacggtgt acggatattt cgtccgccgc tgcggtgacc gcggagccgc
     5401 agaagatctg acgtcggaaa cctttctggc ggcgatggac gccgcgcgga aagacgctcc
     5461 gccgacgatc acggtgccct ggctgatcgg cgtggcgcgg cacaaactcg ccgaccacta
     5521 caggcgaagg gccgaccggt tcaccgttcc ggtcgccgag ccgcccgaac cggtagacgc
     5581 ggccgacggg tgggacgccg aactggatcg gatagtggcc gagagcgtgc tggccaaact
     5641 gcccgaacac caccggaccg tgcttgcact gcgctacatg gacgactgca gcgtgccgca
     5701 gtgtgccgag ttgctcggtc gcactgtgca cgccacagac gcgctgctgg tgcgcgcccg
     5761 tcgcgccttc agatcgcact acccgggacc ggaaggagga acgtcgtgaa caccgaggac
     5821 gcgaggagcg gtaaagacaa cagccacgat ccgctgaccg cgctgcacgg cggcgagctt
     5881 ccggtccaac ccgatccggc tttcgcggcg cgactgcgca cacgcctgga atcggttctg
     5941 tccctgccca accgaacagg agaagtcgat atgagcggca ccgataccgc catcgctgaa
     6001 ttgaacgatc ccacctctgc gcccgacgtg gccaccgcac caccacgtgc tgcggcgctg
     6061 ccctacctct ctgtcgcaaa cgcacgtgag gccattgcct ggtacaccga gaccttcggc
     6121 gcgtcgctcg tcggcgagat gtacgagatg gacgacggcc gcgtcgggca cgcggagctg
     6181 gagatcggtg acggcgtaat ctatctggcc gacgagtatc ccgagatcgg cctgaaagca
     6241 cccgcgccgc aggcgaattc ggtgagcctg atgttgccgg tggccgacac cgacgccacc
     6301 ctgacgcggg cgcgggagct tggggcggcg gtgcagcggg agccgtatga gaattacggt
     6361 gcccgcagtg ccaccatcat cgacccgttc ggacatcggt ggatgctcag cggaccagtg
     6421 accggcgcgc cgattcagat tcagcacggc gacgtcggct acgtctcggt gtggacgccg
     6481 gactccgacc gggcagccgc cttctatggc cacgtcctcg gctggacctt cgatgcggaa
     6541 accaaccagg tcaccaacac cagggagcgc atcggcatcc atagcgtcga cgcaccgccc
     6601 accctgttct gctgctacgc ggtggccgac ctcgacggcg ccaggcaggc gatcgtggac
     6661 ggcggcggaa ctgtcgacag aatcgagcag ttcgacttcg gcacgctccg tggcgccacc
     6721 gactcgcacg gggcgtcgtt cgccgtcttc cagccgaacc cgggtcagcc caggcccgag
     6781 ctcaacggtt cagggccagg cgaactttcg tacatcacct acgaggtggt cgactcggct
     6841 gccttcaaag cgttctacag ccgcgtgttg ttctggacct tcgaacccgg ccgtatcgcc
     6901 gacggctggc aggttcagca gactcacccg atggcaggcg tcgcgggtgg caacgcgcat
     6961 caggtgacgg tgccgatgtg gacggtggac gacatcgacg cagcggtcgc ccgcgtgcgg
     7021 gaggcaggcg gcacggtcat cgacgaaccg tcgcagcagc cctacggcaa gtcagcgcag
     7081 tgcaccgacg accaaggcag ccggttctac ctcggcgagc actagcaagc cgtactttgc
     7141 tagccgagca cgtcggcgat cggcgcgccg gaggcgatct tgctgcgcac cttcatcacc
     7201 ttgcccggca tgccgccgcc gacgaccccg gccacgacgc cgtcgcgttc gtagaacgcc
     7261 agaaacttcc tgccgtcgtc ctcgaccacg tgcactgtgt cgtcggcctg cggttctccg
     7321 aggcactgaa tcttgacgtc gtactggtcg ctccaaaaat agggcaccga caccgtcgcc
     7381 ggtatttcct tacccagcat cgccggcacc agtacccgcg cctggtcggc gacattgctc
     7441 caatgctcaa cgcggacttg gtgtcccact ggatgccgcc acgacgccac gtcgccgatc
     7501 gcccacacat gggcggtggc ggtgcggccg acgtcatcgc agacgacgcc gttgtccagg
     7561 tcgatgccgc tgccttcgag ccagtcgatg gccggcttcg atccgatccc gacgaccacc
     7621 aggtcggcg
//
LOCUS       DASYLX010000168         5958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1214234,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000168 DASYLX010000000
VERSION     DASYLX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214234"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGP27_04105"
     CDS             complement(<1..793)
                     /locus_tag="VGP27_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEV7850534.1"
                     /translation="MRRTARALASVGILVATALYTPGGLAAAEPSSPDSVGSLVAAIA
                     NVNQKLQELGAAVQAQQEGVNKAIVDVQTARDNAAAAEQDVDASKHAVDDSTAAITSA
                     QKRFDTFAASTYVSGPSSSYLTAADPADIIDTASAGQTLATSAQQIVANLQKARTEQV
                     NKESAARLAKDKAEQATVNAQSSQDAAVSALKSAQQTFADQQGRLRELTAERNAAQAK
                     LAEARAWSAPASGRSKPVAPLANPSANWDRAPGTPAPAAGNWDQSL"
     gene            complement(980..1516)
                     /locus_tag="VGP27_04110"
     CDS             complement(980..1516)
                     /locus_tag="VGP27_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6676 family protein"
                     /protein_id="HEV7850535.1"
                     /translation="MTGPHVIPFLPSYIPPDVDMNQVLADVGDDGVSVPPADAKAVPE
                     LRQVIADAHQHGIDLKIVAIASNPPIDTPLRDIATEVGQAYPGSTVLAMSPMWAGSYS
                     KEFDRATLEAGQDIAKNGDPVQSSKNFVSELTTPHFPWTPFSVMLIVLVLAAAVATRS
                     LQVRSKADAAEIEPKASE"
     gene            1676..4486
                     /gene="acnA"
                     /locus_tag="VGP27_04115"
     CDS             1676..4486
                     /gene="acnA"
                     /locus_tag="VGP27_04115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV7850536.1"
                     /translation="MSSKDSFGAHDTLKVGDKSYEIYRLDAVPGTEKLPYSLKVLAEN
                     LLRTEDGSNITKEHIEAIANWDPDADPSVEIQFTPARVIMQDFTGVPCIVDLATMREA
                     VGDLGGDPDKVNPLAPADLVIDHSVIADLFGTADAFERNVEIEYERNGERYQFLRWGQ
                     GAFNDFKVVPPGTGIVHQVNIEYLARVVWERDGVAYPDTCVGTDSHTTMENGLGVLGW
                     GVGGIEAEAAMLGQPVSMLIPRVVGFRLTGERPAGVTATDVVLTVTEMLRKHGVVGKF
                     VEFYGDGVAEVPLANRATLGNMSPEFGSTAAIFPIDQVTIDYLKMTGRSEEQLALVEA
                     YAKEQGMWHDPEREPKYSEYIELDLGDVVASIAGPKRPQDRIALTDAKTAFRKDIHNY
                     VEENLPVEHTKLDEAVEESFPASDPAVLSFNDSDAALPSAAVGSNGRPSKPVEVKCEG
                     RGEFVLDHGAVVIAAITSCTNTSNPEVMLGAALLAKNAVEKGLTSKPWVKTTMAPGSQ
                     VVTDYYEKAALWPYLEKLGFYLVGYGCTTCIGNSGPLPEEISKAINDCDLSVAAVLSG
                     NRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFESEPLGKDDSGTDVYLKDIWPS
                     QQDINDTIASAISTEMFTKNYADVFKGDERWRNLPTPSGNTFEWDENSTYVRKPPYFD
                     GMPPEPEPVTDITGARVLALLGDSVTTDHISPAGSIKPGTPAAQYLDEHGVDKKDYNS
                     YGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTNGGKQAFIYDAAQNYAEQGI
                     PLVVLGGKEYGSGSSRDWAAKGTSLLGVRAVITESFERIHRSNLIGMGVIPLQFPEGE
                     SAASLKLDGTETFDITGIEALNDGKTPKTVHVKATKSGDADGSDNTVEFDAVVRIDTP
                     GEADYYRNGGILQYVLRHMLKS"
     gene            4528..5091
                     /locus_tag="VGP27_04120"
     CDS             4528..5091
                     /locus_tag="VGP27_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850537.1"
                     /translation="MPRVTDDHLAARRRQILDGARRCFAEYGYDRATVRRLEQTIGLS
                     RGAIFHHFRDKDNLFFELAHEDAERMADVASREGLIQVMRDMLAAPEQFDWLATRLEI
                     ARKLRNDPAFNKGWSERSAELSAATTERLRRQKRAGRLRDDVPSEVLHMYLDLVLDGL
                     VARLASGDDPEKLGAVLDLVETSVRQP"
     gene            complement(5088..5282)
                     /locus_tag="VGP27_04125"
     CDS             complement(5088..5282)
                     /locus_tag="VGP27_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850538.1"
                     /translation="MKKLNKSRDQLLQELRNAYEGGASIRTLVATTGRSYGSIHSMLR
                     ESGTTMRSRGGPNHRTRRVS"
     gene            complement(5382..>5958)
                     /locus_tag="VGP27_04130"
     CDS             complement(5382..>5958)
                     /locus_tag="VGP27_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850539.1"
                     /translation="AGVDLAIDRGSRVVVLGLNGAGKTTLLRLLAGVETPDTGGLEPG
                     HGLKIGYFAQEHDTLDDHATVWENIRHAAPDTGEQDLRGLLGAFMFSGRQLDQPAGTL
                     SGGEKTRLALAGLVASTANVLLLDEPTNNLDPASREQVLDALRSYRGAVVLVTHDPGA
                     AEALDPQRVVLLPDGTEDFWSQDYAELIELA"
BASE COUNT         1109 a         1922 c         1908 g         1019 t
ORIGIN      
        1 acaggctctg gtcccagttc cccgcagcgg gcgcaggagt acccggcgcg cggtcccaat
       61 tcgccgacgg gttcgccagc ggcgccacgg gtttcgaccg gcctgatgcc ggcgccgacc
      121 aagcgcgggc ttcggccagc ttggcctggg cggcgttacg ctcggcggtc aactccctca
      181 gccggccctg ctggtcggcg aaggtctgct gggcggactt caacgcggac accgccgcgt
      241 cctggctgga ctgcgcgttc accgtggcct gctcagcctt gtccttggcc agccgtgccg
      301 cggactcctt gttgacctgc tcggtgcgcg ccttctgcaa gttcgccacg atctgctgtg
      361 cgctagtggc cagcgtctgg cctgccgaag cggtgtcgat gatgtcggcg gggtcggccg
      421 ccgtcagata cgaactcgac ggcccgctga cataggtcga cgcggcgaag gtgtcgaatc
      481 gcttctgcgc ggacgtgatc gcggcggttg agtcgtcgac cgcgtgtttg ctggcatcca
      541 catcctgttc agccgccgcc gcgttgtcgc gcgcggtctg cacatcgacg atggccttgt
      601 tgacgccttc ctgctgggcc tgaacggcag cgccgagttc ctgcagtttc tggttgacgt
      661 tggcgatagc tgcgaccagc gatccgacgg agtccggact gctgggttcg gcggccgcta
      721 atccgccggg cgtgtaaagg gcggtcgcca cgaggatgcc gacggacgcg agcgcgcgag
      781 cggtgcgtct catccgactc gggtctcctc gcagaagtca caaaaacaac aactacacca
      841 tcaggtactt ctgcaccgta cgtcacaact gacgctaacg aaactctagt cacaaactac
      901 atatctgtaa ttaaacggag tgtgaccgaa tggtgatgtt cgaattcgcc gtctgctcat
      961 gcccagcgtt atgccggcct tattcggagg ctttcggctc gatttcggcc gcgtcggcct
     1021 tgctccggac ctgcagcgat cgggtcgcga cggcggcggc caaaaccagg acaatcagca
     1081 ttacggagaa tggtgtccac ggaaaatgag gcgtcgtcaa ctcgctgacg aaattcttcg
     1141 acgattgaac ggggtcgccg ttcttcgcga tatcttggcc ggcctcgagt gtcgcccgat
     1201 cgaactcctt gctgtagctg cctgcccaca tcgggctcat cgccagcacg gtcgagcccg
     1261 ggtacgcctg ccccacctcg gtcgcgatat cgcgcagcgg ggtgtcgatc ggcggattgg
     1321 aggcgatggc cacgattttg agatcgatgc cgtgctggtg tgcgtcggcg atcacctggc
     1381 gcaattcggg cacagctttc gcatccgcgg ggggcacgct cacgccgtca tcgccgacat
     1441 ctgcaagcac ttggttcata tcgacgtccg gcgggatata cgacgggagg aacgggatca
     1501 cgtgcgggcc ggtcacagga gaaccgtacg cgatacgcac cgcggaaagt ggcagcacgc
     1561 acccgtcaag acaagactgg aaccgtccag gcttgcctta caggacaagc gtactgttag
     1621 attggaagcg ccgccgacac agcccgtcgc ggcgattact tagccgggag ttgatgtgag
     1681 cagcaaggat tcgtttggag cccacgacac cctgaaggtc ggggacaaga gctacgagat
     1741 ctaccgcttg gacgcggtac ccggaaccga gaaactgccc tacagcctca aagtgctcgc
     1801 cgagaatcta cttcgcaccg aggacggctc caacatcacc aaggagcaca tcgaggcgat
     1861 cgctaactgg gatccggacg ccgatccgag cgtggagatc cagttcaccc ccgcccgcgt
     1921 gatcatgcag gacttcaccg gggtgccctg catcgtcgac ctggccacca tgcgggaggc
     1981 cgtcggcgac ctcggcgggg atcccgacaa ggtcaatccg ctggcccccg ccgatctggt
     2041 gatcgaccac tcggtgatcg ccgacctctt cggcacggcc gacgccttcg agcgcaacgt
     2101 ggaaatcgag tacgagcgca acggcgagcg ctatcagttc ctgcgctggg gccagggcgc
     2161 attcaacgac ttcaaggtgg taccgcccgg caccgggatc gtgcaccagg tcaatatcga
     2221 atacctggca cgcgtggtgt gggagcgtga cggtgtcgcc tatccggaca cctgcgtcgg
     2281 caccgattcg cacaccacca tggagaacgg tctcggcgtg ctcggctggg gcgtcggcgg
     2341 catcgaggcc gaagcggcca tgctcggcca gccggtctcg atgctcattc cccgggtggt
     2401 cggcttcagg ctgaccggcg agcggccggc cggtgtcacc gccaccgacg tcgtgctcac
     2461 cgtcaccgag atgctgcgca agcacggcgt tgtcgggaag ttcgtcgagt tctacggcga
     2521 cggcgtcgcc gaggtgccac tcgccaaccg cgccaccctg ggcaacatga gccccgaatt
     2581 cggctccacc gcagcaattt ttccgatcga ccaggtgacg atcgactacc tcaagatgac
     2641 cggccgcagt gaggagcagc tggcactggt ggaggcctac gccaaagagc agggcatgtg
     2701 gcatgacccg gagcgtgagc cgaagtactc ggagtacatc gaactggacc tgggggatgt
     2761 ggtggcgtcg atcgccggtc cgaagcgtcc gcaggaccgc atcgcgctga ccgacgcgaa
     2821 gacggcgttc cgcaaggaca tccacaacta tgtggaggag aacctgcccg tcgagcacac
     2881 caagctcgac gaggccgtcg aagagagctt cccggccagc gacccggcag tgctttcgtt
     2941 caacgacagt gacgctgccc tgccgtcggc ggccgtcggc tccaacggca ggccgagcaa
     3001 gccggtggag gtcaaatgcg agggccgcgg tgagttcgtc ctcgaccacg gtgccgtcgt
     3061 gatcgcggcg atcacatcgt gtaccaacac ctccaatccc gaggtgatgc tcggcgcggc
     3121 gctgctggcc aagaatgccg tcgagaaagg tctgacgtcc aagccgtggg tcaagaccac
     3181 gatggcgccg ggttcgcagg tggtcaccga ctactacgaa aaggcggcgc tgtggccgta
     3241 tctggagaag ctcgggttct atctggtcgg ctacggctgc acgacctgca tcggcaactc
     3301 cggcccactg ccggaggaga tcagcaaagc gatcaatgac tgcgatctgt cggtcgctgc
     3361 cgtgctgtcg ggcaaccgca acttcgaggg ccggatcaat cccgacgtga agatgaacta
     3421 cttggcgtct ccgccgctgg tgatcgcata tgccttggca ggcaccatgg acttcgactt
     3481 cgagtcggag cccctcggca aagacgactc gggcaccgat gtctatttga aggacatctg
     3541 gccgtcgcag caggacatca acgacaccat cgcctcggcg atcagcacgg agatgttcac
     3601 caagaactac gccgacgtgt tcaagggcga cgagcggtgg cgcaatctgc cgacgccgag
     3661 cggcaacacg ttcgagtggg acgagaactc cacgtatgtc cgcaagcccc cgtacttcga
     3721 cggcatgccg cccgaaccgg agcctgtcac cgacatcacc ggcgccaggg tgcttgcgct
     3781 gctgggggat tcggtgacca ccgaccacat ctcccccgcg ggcagcatca agccgggcac
     3841 acctgccgcg cagtatctcg acgagcacgg ggtggacaag aaggactaca actcctacgg
     3901 ctcacggcgt ggaaaccacg aggtgatgat tcgcggcaca ttcgcgaaca tccggctgcg
     3961 caaccagctg ctcgacgacg tgtccggcgg ctacacccgc gatttcacca acggcggcaa
     4021 gcaggcgttc atctacgatg ccgcgcagaa ctacgccgag caggggatcc cgctggtcgt
     4081 gctggggggc aaggagtacg gatcgggttc gtcgcgcgac tgggccgcca agggcacaag
     4141 tctgctgggt gtgcgcgcgg tcatcaccga atcgttcgag cgcattcatc gctccaacct
     4201 gatcggcatg ggtgtcatcc cgctgcagtt ccccgagggc gagtcggcgg cttcgctcaa
     4261 gctggacggc acggagacgt tcgacatcac cggcatcgag gcgctcaacg acggcaagac
     4321 gccgaagacg gtgcacgtga aggccaccaa atcgggcgac gcggacggct cggacaacac
     4381 cgtggagttc gacgcggtgg tgcgcatcga cacccccggc gaggcggact actaccgcaa
     4441 cggcggcatc ctgcagtacg tgctgcggca catgctcaag tcataggcga cacaatcgat
     4501 cagcgctcgc gcgaggagta acacaagttg ccccgggtaa ccgacgatca tctggcggcg
     4561 cgtcgtcgtc agatcctcga tggtgcccgg cggtgctttg ccgagtacgg ctacgacagg
     4621 gccaccgtac gcaggctcga gcagacgatc gggctctcgc gcggcgcgat cttccaccac
     4681 tttcgggaca aggacaatct gttcttcgaa ctggcccacg aagacgccga gcggatggcc
     4741 gatgtcgcgt cgcgcgaggg cctgatccag gtcatgcgcg acatgctcgc ggcgcccgaa
     4801 caattcgact ggttggccac tcgcctcgag atcgcgcgca agcttcgcaa cgacccggcg
     4861 ttcaacaagg gatggtcgga acggtcggct gaactgtcgg ctgcgaccac cgagcgattg
     4921 cgcagacaaa aacgggccgg gcgactacgc gacgacgtgc ccagcgaggt actgcacatg
     4981 tatttggatc tggtgctcga cggcctggtc gcgcgactgg cctccggtga cgaccccgaa
     5041 aagctcggcg ctgttttgga cctcgtcgag acgtccgtgc gccagcccta gctgacccgt
     5101 cgcgtgcggt ggttgggccc gccgcgacta cgcatcgtgg tgccggattc ccgcaacatg
     5161 ctgtgtatcg agccatacga tcggcccgtc gtggcgacca gtgtgcgaat gcttgcgccc
     5221 ccctcgtagg cgttccgcaa ttcctgtagc aactgatccc gcgatttgtt cagtttcttc
     5281 atcgacacct ccccgctatg atgccccgac gaatacccag cgtatgagag ccgaaaccat
     5341 actcacgaca gataaaaaca aacggcgtgg cgaatgtcgc gtcaggccaa ctcgatgagt
     5401 tcggcgtagt cctgcgacca gaagtcctct gtcccgtccg gaagcagcac cacccgctgc
     5461 ggatcgagcg cctcggcggc accgggatcg tgggtcacca gcaccaccgc gccgcggtag
     5521 ctgcgaagtg cgtcgagcac ctgctcccgc gacgccggat cgaggttgtt ggttggttcg
     5581 tcgagcagca ggacgttcgc cgttgaggcg acgagacccg caagcgcaag cctggtcttc
     5641 tcaccgccgg acagcgtgcc cgcaggctga tccaactgcc ggccactgaa catgaaggcc
     5701 cccaacaagc ctcgcagatc ctgctccccc gtgtccggag ccgcgtggcg gatgttctcc
     5761 cagaccgtgg cgtggtcgtc gagggtgtcg tgttcctggg cgaagtagcc gatctttagc
     5821 ccgtgacccg gttccagccc accggtgtcc ggtgtctcga cgccggcgag caggcgcagc
     5881 agcgtggtct ttcccgcgcc gttgaggccg agcaccacga cccgagagcc gcggtcgatg
     5941 gccagatcga caccggcg
//
LOCUS       DASYLX010000169         5063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3738286,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000169 DASYLX010000000
VERSION     DASYLX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738286"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGP27_04135"
     CDS             complement(<1..303)
                     /locus_tag="VGP27_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7850540.1"
                     /translation="MVEDQVLDRAYGALADPTRRWLLTTLRQGDARISDLAAPLPMTF
                     AGVSRHIGVLESAGLVRREIRGREHWLSLQPDGLTMAQQWISEQTEFWSTRADALSA"
     gene            397..1092
                     /locus_tag="VGP27_04140"
     CDS             397..1092
                     /locus_tag="VGP27_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEV7850541.1"
                     /translation="MQTPPIVSAQQWEGARREMLVKEKEVTRARDALAAMRRRMPWTP
                     VEKTYAFEGPDGRVSLLDLFDGRRQLIVYRAFVDPGVHGWPEHGCVGCSLMADHIGNL
                     AHLNARDTTLVYASRGSQSDLARVKARMGWGHPWYTIVPGPDGAFDVDFGVDEWHGTN
                     AFIRDGDRIFRTYFIDNRGDEMFVNTWHFLDMTALGRQETWELSPEGYPQTKPYEWWR
                     WHDEYGDESSPKE"
     gene            1089..1766
                     /locus_tag="VGP27_04145"
     CDS             1089..1766
                     /locus_tag="VGP27_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEV7850542.1"
                     /translation="MTAVSNTDPRQVARFYLDTKERITALVSGLDDSALSTTVAACPG
                     WSVRDVLAHCAAVADDLGQGRLTGPPTDVETAAQIARFEGHDMAGIVEVWTDAAACLG
                     RLAENAGVEPPVGDVTSHEHDVRGALGRPGARDSDAVRYSSDRLLANLRTPVPLRVTV
                     EDAEYRCGPADGADLRLRTTRFEALRWRTGRRSRGQLAAMDWSDDPAPVLDHLCMFGP
                     AAADLIE"
     gene            1783..2538
                     /locus_tag="VGP27_04150"
     CDS             1783..2538
                     /locus_tag="VGP27_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEV7850543.1"
                     /translation="MKNESPHRHSVNNAPSPAAVDRATFEAEIDTLRAREKAHIRQGD
                     AIAASRRALPMVEVDANLELIGPDGPVTLLEAFDGRRQLLAYYFMWHTGASAPEQCEG
                     CTWVTTQVQELSYLHSRDITYAVFCQGPYEQSARYRDFMDWHMPWYSALPSLDSLLVG
                     RTAGMMHIVSYLRDGDRVFETYWTTLRGVEAMDYSLALMDLTVYGRQEPWEESPAGWP
                     QDWRVDGNNTRIDGRPIPQWSRLEAGRSDDLAG"
     gene            complement(2699..4459)
                     /locus_tag="VGP27_04155"
     CDS             complement(2699..4459)
                     /locus_tag="VGP27_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850544.1"
                     /translation="MFDLKITGGTVVDGTGTDRFQADVAVKDGKIVEVRGRGPGSPPL
                     EGEAAETIDATGRIVAPGFVDIHTHYDGQVSWDSLLEPSSGHGVTTVVAGNCGVGFAP
                     VRPGSEQWLIGLMEGVEDIPGTALTEGISWGWESYPQYLDVIGKQNFAIDVGSQIAHG
                     SVRAYAMGERGARNEPASPEDIAAMARIVQEGIEAGALGFSTSRTIGHRAIDGEPVPG
                     TYAAEEELFGLGRAMAAGGRAVFELAPQGVAGEDIIAPKKELEWMQRLGAEIDRPISF
                     GMIQVDAAPDLWREQLDISAAAHAAGSQLYPQIAARPFGMLFGFPGHHAFTHRPTFRR
                     LKAECSREELAQRLADPAVKAAILAEDDLPPDPKLLFDNMFALVQYSLGRLYALGDPP
                     DYEPTPDRAVEKIAADRGEDPLATLYDLMLESDATSMLMLPFYNYYYGNHDAIREMLT
                     HPAGVSGLSDGGAHCGMICDASYPTFLLTHWARDRHRGDKLPLEYVVRKQSHDTAQLF
                     GLSDRGVIEVGKRADVNVIDMNALTLHPARMAYDLPAGGQRLVQGATGYTATIVNGVV
                     TRRDGVDTGARPGRLVRGAR"
     gene            4578..>5063
                     /locus_tag="VGP27_04160"
     CDS             4578..>5063
                     /locus_tag="VGP27_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7850545.1"
                     /translation="MVTQAAQVKPAVADLTDAQQEDAIGTPPKRPTLVPAERYYSAAF
                     ARLEVERMWPKVWQVACTVDHVAAPGDFFEYRCGPYSVLVVHGDDGELRAFQNVCRHR
                     GNSLCSGAGSDLRELKCGYHGWTWDLTGALKRVPNRKGFGSLRISEFPLIAAKVDTWE
                     RL"
BASE COUNT          847 a         1673 c         1686 g          857 t
ORIGIN      
        1 cgccgagagc gcgtccgcac gtgtcgacca gaactccgtc tgctcgctga tccactgctg
       61 agccatcgtc aatccgtccg gttgcaggga gagccagtgc tcgcggccgc gtatctcccg
      121 tcgcaccaac ccggccgact caagcacgcc gatgtggcgc gaaacgcccg cgaacgtcat
      181 cgggaggggc gcggcgaggt cggagattcg ggcatcgccc tggcgcaatg tcgtcaacag
      241 ccaccgtctg gtggggtcgg cgagcgcgcc atacgcacga tccaaaacct gatcttcaac
      301 cattctgttg actataacgg cctctcgtga tgtgctgaag aaagttgttc aaccgaaatg
      361 ttgaatgttt cgacggcaga ccggtgagga gaccgcatgc agacgcctcc gattgtctcg
      421 gcgcagcagt gggagggtgc gcggcgcgag atgctcgtca aggagaagga agtcacccgg
      481 gcgcgtgacg cgctcgcggc aatgcggcgg cgaatgccgt ggacgccggt cgagaagacg
      541 tatgccttcg aggggccgga cggcagagtc agcctgctgg acttgttcga cgggcggcgc
      601 cagctgatcg tgtaccgcgc cttcgtcgat ccgggcgtgc acgggtggcc ggaacacgga
      661 tgtgtcggat gctcgttgat ggcagaccat atcggcaacc tggcccacct caatgcccgc
      721 gacaccactc tggtctacgc ctcgcggggt tcgcagtcag accttgcacg ggtgaaggcg
      781 cggatgggtt ggggccaccc gtggtacacc atcgtgccgg gaccggatgg cgccttcgac
      841 gtcgacttcg gcgtggacga atggcacggc accaacgcgt tcatccgcga cggtgaccgg
      901 atcttccgca cctacttcat cgacaaccgc ggcgacgaga tgttcgtcaa cacctggcac
      961 tttctggaca tgaccgcgct gggacggcag gagacctggg agctttcacc cgagggctac
     1021 ccccagacca aaccctacga atggtggcgc tggcacgacg aatacggcga cgaatcgtcc
     1081 ccgaaggaat gaccgccgtg tcgaacaccg acccgcgcca ggttgcccgg ttttatctcg
     1141 atacaaagga gcgcatcacc gcgttggtga gcggcctcga cgactccgcg ctgtcgacca
     1201 cggtcgcggc ttgtcccggc tggtcggttc gcgatgtgtt ggcgcattgt gcggccgtcg
     1261 ccgacgactt gggtcaggga cgcctgacgg gaccgcccac cgacgtggag accgccgcgc
     1321 agatcgcccg cttcgaaggc cacgacatgg ccggcatcgt cgaggtctgg accgacgcgg
     1381 cagcctgcct cggccgtctc gccgagaatg ccggcgtgga accgcccgtc ggcgacgtca
     1441 ccagccacga gcacgatgtc cgcggtgcac tcggcaggcc gggcgcccgc gactccgacg
     1501 cggttcggta ctcctcggat cggctgctcg ccaaccttcg gacgccggtg ccgttgcggg
     1561 tgaccgtgga agacgccgag taccggtgcg ggccggccga cggcgcggac ttacgtcttc
     1621 gtactacgcg gttcgaagca ctgcgctggc gaaccggtcg ccgcagccgt ggccagctcg
     1681 cggcgatgga ctggtccgac gacccggcgc ccgtgctgga ccacctgtgc atgttcggcc
     1741 cggccgccgc cgacctcatc gaatagcctc gcaggagcga tcgtgaaaaa cgagtcaccg
     1801 catcgccatt cggtcaacaa cgcgccgtcc ccagcggccg tcgatcgagc gacgttcgag
     1861 gccgagatcg acaccctgcg ggctcgcgag aaagcccata ttcgacaggg cgatgcgatc
     1921 gctgcctcgc gccgggcgct gccgatggtc gaggtcgacg ccaacctgga gttgattggg
     1981 cccgacgggc cggtcaccct gctcgaggct ttcgacggac gcaggcagct tctggcctat
     2041 tacttcatgt ggcataccgg cgcgtccgcg cccgaacagt gcgaagggtg cacgtgggtg
     2101 acgacgcagg tgcaggagct gtcctacctg cattcccggg acatcaccta tgcggtcttc
     2161 tgccagggtc cgtatgagca gagtgcccgg taccgcgact tcatggactg gcacatgccg
     2221 tggtactcgg cactcccctc gctcgactca ctcctcgtcg gccgcaccgc gggcatgatg
     2281 cacatcgtca gctacctccg cgacggcgac cgcgtcttcg agacctactg gacgacgctc
     2341 cgcggcgtcg aggcgatgga ttacagcctc gccctgatgg atctcacggt gtacggacgc
     2401 caggagccct gggaggagtc gccggccggc tggccgcagg actggcgcgt cgacggtaac
     2461 aacacccgca tcgacggaag gccgatcccc cagtggtcgc ggctggaggc cggacggtcg
     2521 gacgatctcg ccggctaacg ccggaagaat gcattcaggc cgtatcaatc tcagagactt
     2581 cgcagttcga acatgggcgc aacgagcacc atcgagacgc cgccgtaagt gccggacacc
     2641 tccgtcgcag cgcccgcggt gaaggccggc ggctagtttc gtaccgcaag acgacgttct
     2701 agcgcgcccc gcggacaagt cggccgggac gtgcgccggt gtcgacgccg tcgcggcggg
     2761 tgacgactcc gttcacgatc gttgcggtgt agccggtggc gccctggact aggcgctggc
     2821 cgcccgcggg caggtcgtag gccatccggg cggggtgcag cgtcagcgca ttcatgtcga
     2881 tgacgttgac gtcagccctc tttccgacct cgatgacacc gcggtcggag agcccgaaca
     2941 actgcgcagt gtcgtgagac tgcttgcgca ccacatactc cagcggcagc ttgtcgcctc
     3001 ggtggcggtc ccgcgcccag tgcgtcagca ggaacgtggg gtaggacgcg tcgcagatca
     3061 tgccgcagtg cgcgcccccg tcggacaggc ccgagacccc ggcggggtga gtgagcatct
     3121 cccggatcgc gtcgtggttg ccgtagtaat agttgtagaa gggcagcatc agcatcgatg
     3181 tcgcgtcgga ctcgagcatc aggtcataca gcgtggccaa gggatcctcg ccgcgatcgg
     3241 cggcgatttt ctcgacggcc cggtcagggg tcggctcgta gtcgggagga tcgccgagcg
     3301 catagaggcg gccgagagag tattggacga gtgcgaacat gttgtcgaac aacagtttcg
     3361 ggtcgggtgg cagatcgtcc tcggcgagga tcgccgcctt gaccgcggga tcggcgagac
     3421 gctgcgccaa ctcctctcgg ctgcattcgg ctttcaaccg ccggaaggtc ggacggtgag
     3481 tgaacgcgtg gtggccaggg aaaccgaaca gcatgccgaa cggtcgcgcc gcgatttggg
     3541 gatacaactg gctgcccgcc gcatgagccg ccgccgagat gtccagttgt tcgcgccaga
     3601 ggtcgggcgc cgcgtcgact tggatcatgc cgaacgagat gggccggtcg atctcggcgc
     3661 cgaggcgctg catccactcc agttccttct tcggcgcaat gatgtcctcg ccggccactc
     3721 cctgcggggc cagctcgaag acggccctcc cacctgccgc catggcccgg ccgagtccga
     3781 acagttcctc ttctgcggcg taggttccgg gaacgggttc gccgtcgatg gcccggtgac
     3841 cgattgtgcg cgaggtcgag aagccgagcg cgcctgcctc gattccttcc tggacgatgc
     3901 gcgccatcgc ggcgatatcc tccggtgagg cgggctcgtt gcgtgcgccg cgctccccca
     3961 ttgcataggc gcgaaccgaa ccgtgggcta tctggctgcc cacgtcgata gcgaaattct
     4021 gctttccgat gacgtcgagg tattgggggt agctctccca gccccacgag atgccctcgg
     4081 tgagtgcggt gcccggaatg tcctctacgc cctccatcag gccgatcagc cactgctctg
     4141 acccgggccg gaccggggca aacccgacgc cgcagttccc ggcgaccacg gtggtcacgc
     4201 cgtgtccgct cgagggctcc aacaggctgt cccagctgac ctggccgtcg tagtgcgtat
     4261 ggatgtccac gaagccgggc gcgacgatcc ttccggtggc gtcgatcgtt tcggccgcct
     4321 ccccttccag cggcggactg cccggcccgc gacctcgaac ctcgacgatc ttgccgtcct
     4381 tgaccgccac gtcggcctgg aaacggtccg tgcccgtccc atcgacaacg gttccgcctg
     4441 tgatcttgag gtcgaacacg tttactccct accaacgtct cgccggccgc aacttctgtc
     4501 ccaggtagcc ggccgggcgg ctacggtggt caatgtaaca ccgttacaga ctcgtggcga
     4561 cggatcaaaa ggggttgatg gtgacacagg cagcgcaggt gaagcccgcg gtcgccgacc
     4621 tcaccgacgc ccagcaggaa gacgcgatcg gcacacctcc gaagcgcccc accctggtgc
     4681 ctgcggagcg gtactactcc gcggcgttcg cgcggctcga ggtcgaacgg atgtggccga
     4741 aggtgtggca agtcgcctgc acggtcgacc acgtggccgc gcccggtgac ttcttcgagt
     4801 accggtgcgg gccgtactcc gtgctcgtag tccacggcga cgacggcgag cttcgcgcgt
     4861 tccagaacgt gtgtcgccac cgcggcaact cgttgtgctc cggcgccggc tccgacttgc
     4921 gcgagctgaa gtgcggatat cacgggtgga cgtgggatct caccggtgcg ctcaagcgag
     4981 tgcccaaccg caaggggttc ggctcgcttc ggatctctga attcccgttg atagcggcga
     5041 aagtcgatac ctgggagcgg ctc
//
LOCUS       DASYLX010000170         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3299476,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000170 DASYLX010000000
VERSION     DASYLX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299476"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VGP27_04165"
     CDS             <1..780
                     /locus_tag="VGP27_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7850546.1"
                     /translation="GLIDFELKRLGYVTAMPLGVELRGARVSGRTALERAQSALEAHS
                     ELRSQEGSSAFEEPEPAESLSGLLVSRDHSAPTPTADHLPGLSALLRVSRDVGAVVLG
                     GVPASSLRNGGTPWDAAARQSVADQLRAAAERAALPAEVVERLNEASELLGIEIVPQS
                     LCFEHNDIADVLVPGARICFTGTVQDTAGRIVERWEMEELAASAGLKPVRSVSKTRCE
                     VLVTAEAGSQSGKARKAQEYGKAVFTADEFLAWVTKLAARQ"
     gene            844..1860
                     /locus_tag="VGP27_04170"
     CDS             844..1860
                     /locus_tag="VGP27_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7850547.1"
                     /translation="MQVDAMTVPQPLGKAGDLARRVQDAGFSGLLFTETGRTAYLNAA
                     VASQAAPGLELSTGVAVAFPRSPFVTAATAWELQESTGGRFRLGLGTQVRTHVVRRYG
                     MEFERPGPRLRDYVRAVKACFRAFRTGKLDHHGDFYDLDFITPQWSAGPIDAPDPKVD
                     VAAVNPWMLRMAGEVADGVHVHPLGEPGYIARHVLPNVAAGAQNSGRDPSDIAVIVPV
                     LTIVGDTDEERDKERESVRFSMSFYGSTPNYSFIWDDAGFDGTTARIREKQKAGDFEG
                     MAAQITDDHIATFATESTWDGLADALREKYGRTATRIVLYNALGDEERVERYGVVAQR
                     LSGV"
     gene            2174..2776
                     /locus_tag="VGP27_04175"
     CDS             2174..2776
                     /locus_tag="VGP27_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850548.1"
                     /translation="MIPTSGCGAQSPWSSREAELLAVTLELLQEHGYDRLTVDAVATT
                     ARASKATIYRRWPTKAELVLAAFVEGTRQVAVDPDTGTLREDLLRLGEQITAHVSTHA
                     GTIRAVLVEVSRSAELDAMVQEQFFDQRKALMAHALKRAVERGEITASAVTEEFWDVL
                     PGYLIYRSVLAGRVPSRRTVEDLVDNLLMPGLTRHNAPRG"
BASE COUNT          480 a          889 c         1025 g          488 t
ORIGIN      
        1 ggcctgatcg atttcgagct caagaggctc ggctatgtga cagcgatgcc gctcggcgtc
       61 gagctgcgcg gcgcgcgggt gtcgggccgg acagcgctgg agcgggcgca gtctgcgctg
      121 gaggcgcata gcgagctgag gtcgcaagag gggtcgtcgg cgttcgagga acccgaaccg
      181 gcggagtcgc tttcggggct gctggtgagc cgcgatcact ctgcgccgac acctacggcg
      241 gatcacctgc cgggattgtc ggcactgctg cgtgtcagca gggacgtcgg cgcggtggtg
      301 ctgggcggag tgccggcgtc gtcgctgcgc aatggcggca cgccgtggga tgcggcggcg
      361 cggcagtcgg tggcggacca gctgcgggca gccgctgagc gggctgcgct gcccgcggag
      421 gtggtggagc gactgaacga ggcgtccgag ctgctcggca tcgaaatcgt gccgcagtca
      481 ttgtgtttcg aacacaacga catcgccgac gtgttggtgc caggagcacg catctgcttc
      541 accggcacgg tgcaggacac cgccggtcgc atcgtcgagc gctgggaaat ggaagagctg
      601 gccgcctcgg ccgggctgaa accagtcagg tcggtttcca agacgcgctg tgaggtgctg
      661 gtcacagccg aggcgggcag tcagtccgga aaggcgcgca aggcgcagga atacggcaag
      721 gcggtgttca ccgcggacga gtttctcgcc tgggtgacga agttggcggc gaggcagtga
      781 gcgctacacg ttctcgccgc cgccgcgatt tcatctaaca cccccgggcg tactgttcga
      841 cccgtgcagg tagacgcgat gaccgtcccg cagccgctgg gcaaggccgg tgacctggcc
      901 agacgggtgc aggacgcggg cttctcgggc ttgttgttca ccgagacggg tcggaccgcg
      961 tacctcaacg cggcggtggc ttcgcaggcg gcacccggtc tcgagctgtc gacgggagtt
     1021 gcggtggcct tcccgcgcag tccgttcgtg accgcggcga cggcgtggga attgcaggag
     1081 tcgacgggcg ggagattccg gctcggactg ggcacgcagg tgcgtaccca tgtggtgcgg
     1141 cggtacggca tggagttcga gcgtccgggc ccgcgactgc gcgactacgt gcgggcggtg
     1201 aaggcgtgct tccgtgcgtt ccggacgggc aagctggacc atcacggtga tttctacgac
     1261 ctcgacttca tcacgccgca gtggagcgca ggaccgatcg acgcgcccga tcccaaggtc
     1321 gacgtcgcgg cggtgaaccc gtggatgttg cggatggccg gcgaagtggc cgacggcgtg
     1381 catgtgcatc cactcggcga gccgggctac atcgctcgcc acgtcctgcc gaatgtggcg
     1441 gcgggtgcgc agaattcggg tcgcgacccg tcggacatcg cggtcatcgt gccggtgctg
     1501 acgatcgtgg gcgacacaga cgaagaacgg gacaaagagc gtgagagtgt ccgattcagc
     1561 atgagcttct acggaagcac gcccaactac tctttcatct gggacgacgc aggtttcgac
     1621 ggcacgacgg cgcggattcg cgagaagcag aaggctggcg acttcgaagg gatggcggcg
     1681 cagatcaccg acgaccacat cgccaccttc gccacggagt cgacgtggga cggtctggcc
     1741 gacgcgctga gagagaagta cggccgaacc gcaacgcgca tcgtgctgta caacgccctc
     1801 ggcgatgaag aacgagtcga gcgatatggc gtcgtcgctc aacgcctgtc gggcgtgtga
     1861 cacggattgt ccgcgcgcat cagggcggct acgacgtctg agagaggccc gtcctcgttt
     1921 gcgcccttgc ggtgggcgaa gtatgcccgc actgcacgga tctcttcttg cagtacctcg
     1981 tcatcggtca tttctgggtc ccttgccgtg agccgcttgc cggaatgaac acgacgctat
     2041 tgcgcagcgt ttgcggtggc gcgagtagtc gactacccga acgggcgggg acgccgccgt
     2101 ctgcgcgttt tctcgaatag tgcagcagaa tggccgatac tcgttcgcac atgtcccctg
     2161 gttcggaggc cccgtgatcc cgacgtccgg ttgtggcgcg cagtcgccat ggtcgtcacg
     2221 ggaggccgag ctgctcgcgg tcacgctgga gctgctccag gaacatggct atgaccggct
     2281 gaccgtcgac gcggtggcca caaccgcacg tgccagcaag gccacgatct accgccgatg
     2341 gcccaccaag gccgaactgg tgctggccgc attcgtcgag ggcacccggc aggtcgcggt
     2401 cgaccccgac accggcacgc tgcgcgagga tctgctgcgg ctgggagagc agatcaccgc
     2461 tcacgtcagc acccatgccg gcacaattcg ggcggtgctc gtggaggtct cccggagcgc
     2521 cgagctcgac gccatggtgc aggaacagtt cttcgaccag cggaaagccc tgatggcaca
     2581 cgctctcaag cgggcggtcg agcgcggcga gatcacggcc tccgccgtca ccgaggaatt
     2641 ctgggacgtg ttgcccggtt acctgatcta tcgatcggtc ctggccggcc gtgtcccgtc
     2701 gcggcgcacc gtcgaagacc tcgtcgacaa cctgctcatg cccggtctca cccgccacaa
     2761 cgccccgagg gggtgagggt cagcgaacat ctgtacggct tgtgcggtcc ggtcccgtat
     2821 cgtactgtca actaagttga tatagctcgc ctgctcggaa atgtcgtccc gtccgtaaac
     2881 gt
//
LOCUS       DASYLX010000171         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_336576,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000171 DASYLX010000000
VERSION     DASYLX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336576"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGP27_04180"
     CDS             <1..187
                     /locus_tag="VGP27_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV7850549.1"
                     /translation="DVAGENRKVNIGMLPEETFAPGDWVIIHMGFVVEKTDEAGADAA
                     MAGLKMMGSGEDLEATP"
     gene            196..2484
                     /gene="hypF"
                     /locus_tag="VGP27_04185"
     CDS             196..2484
                     /gene="hypF"
                     /locus_tag="VGP27_04185"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEV7850550.1"
                     /translation="MTGRRRLRICVNGVVQGVGFRPFVYTCAAALALTGSVRNDSSGA
                     IVEVEGDAADLDDFLDRLTHRAPPLAVIESVETQEIPVVGGTGFVITGTSRSGGGRTL
                     ASPDVAMCAECATEQRDPADRRYRHAFVNCTNCGPRFTIIASLPYDRASTTMAPFPMC
                     ADCASEYRDPADRRFHAQPVCCPNCGPALSYRAGAGDVVEGESALTHARKLLSDGGVL
                     AVKGIGGYHLACDAGDETAVTELRSRKRRGDKPFAVMVPDLDTAHRVADIDDAAQRLL
                     CGPQRPIVLMPRLAAAPVATSVAPHNPDLGVMLAYTPLHALLFGLSGDAGGPQVLVMT
                     SGNLGGEPICFTDEDALERLSHLADGWLMHDRAILVPCDDSVVRVVDGNQLPIRRSRG
                     YAPLPVALPVAVPPTLAVGGDLKNTFAVAEHKYAWLSQHIGDMDDLATLAAFDSAEQH
                     LEALTGVSPEILVADSHPGYRSTAWAQRHAGGRPVRSVQHHHAHIAAVMAEHGLDGSQ
                     RVLGFAFDGTGYGPDRAIWGGEVLLADYKGYQRLAQLKYVPLAGGDVSVLRPYRMALS
                     HLWAAGLGWDEDLAPVRACPPEERRALRHQLETGLGCAPTSSMGRLFDAVSSLADVRH
                     VVAYEAQAAIELEGLSRNADGQGRPYAFAVDAGRQPAVIDPGPVLAAIIDDQRAGTPA
                     GVIGARFHCAVADLVVELAGMEDTGGQPVALSGGVFQNAMLLRLVLDGLRAKGFRVIT
                     HRHVPPNDGGIALGQLLVGTAG"
     gene            2489..3244
                     /locus_tag="VGP27_04190"
     CDS             2489..3244
                     /locus_tag="VGP27_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEV7850551.1"
                     /translation="MTALHEQKLPEGYTLFARYAFPPNELGYCGPSDSGVLLRGDNPA
                     GVAAHAKQFDGAWPYLHAIADAAGTPDALDADVVRSYWVGGPHLARVDPAALLEKLRH
                     AFKGQATGMLFDLAHPHGTLAHHSFHVFVVYPWVRFLDRDAPTALKVLQDCRIRWGAV
                     ESVDDEHAVIATRPLMFADGILALGEPRSERVRWSKDGISLIGPPAPGDVVSAHWDWA
                     CGVLTDDECAALGEATQTTLDLVNKARNEERNR"
     gene            3241..3984
                     /locus_tag="VGP27_04195"
     CDS             3241..3984
                     /locus_tag="VGP27_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase assembly protein HupF"
                     /protein_id="HEV7850552.1"
                     /translation="MTVTTEPGGGFVDTALSADLAAAALDMARRFHEGATLWVISPQW
                     EPHAHHVAVEFVHPVIMGKRALPSVALVEPDPVAQARVAAQPGDLLIAVASADEPAVI
                     EAMRRAPAWGAHTLWIGSGPRPPAGAANHILWVDSDDPMVPATGRFVLMYHLLWELTH
                     VCFEHPGLLKPPVEECTDEVCVTCSDEGRLGEVVFEPADPMGSALVRTATGEEWVDVT
                     ILGEVAPNDLVLVHAGTAIARLGDEEVNR"
     gene            3981..4670
                     /locus_tag="VGP27_04200"
     CDS             3981..4670
                     /locus_tag="VGP27_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005258645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV7850553.1"
                     /translation="MTTHGKESTESTAFLYPFIDSEETDAGSLLDDLSVSARGKASES
                     ARLQQESLDEYAPALTAAGVEMAERFLRGGRLYTLGNGGSSTDAATLASLFSRPARGR
                     AVAAWSLAADEAIVTALGNDVGFDLIFKRQIIAHARDRDVAIALSTSGNSEDLMSAVT
                     EAKQRGLLTIGFSGHDGGRMAVAEDLDYCYTVHSQSIHRIQESHALLGFRLWSVVQEH
                     MSRPRNGVHTS"
     gene            4667..>4806
                     /locus_tag="VGP27_04205"
     CDS             4667..>4806
                     /locus_tag="VGP27_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEV7850554.1"
                     /translation="MSPTDAMTAAEREDRVLERIDKFRQRRPRLLDDVVTLAHGAGGK
                     SSA"
BASE COUNT          791 a         1619 c         1634 g          762 t
ORIGIN      
        1 cgacgtcgca ggcgaaaacc gcaaggtcaa catcggcatg ctgccggagg agacctttgc
       61 gccgggtgac tgggtgatca tccacatggg tttcgtggtc gagaaaaccg acgaggcggg
      121 cgccgacgcc gccatggccg gactcaagat gatgggcagc ggcgaagacc tggaggcgac
      181 accatgaccg gggccgtgac cgggcggcgc cggttgcgaa tctgcgtcaa cggcgttgtc
      241 cagggcgtcg gcttccggcc gttcgtctac acgtgcgcag ccgcgctcgc cctcaccggg
      301 tcggtgcgca acgacagctc cggcgcgatc gtcgaagtcg agggcgacgc ggccgatctc
      361 gatgacttcc tggacaggct cactcaccgc gcgcccccgc tggccgtcat cgaatcggtg
      421 gaaacgcaag agattccggt cgtcggcggc accggcttcg tcatcaccgg cacgtcgcgt
      481 tccggcggcg gccgcacgct cgcctccccc gatgtcgcga tgtgcgcgga gtgcgcgaca
      541 gaacaacgcg acccggccga ccggcgctac cggcacgcct tcgtcaactg caccaactgc
      601 ggtccccgct tcaccatcat cgcctcgctg ccctatgacc gcgcaagcac cacgatggcc
      661 ccgttcccga tgtgcgccga ctgcgcgagc gaataccgcg atccggccga ccgccggttc
      721 cacgcacagc cggtctgctg cccgaactgc ggaccggcac tgagctaccg ggccggcgcc
      781 ggcgacgtgg ttgagggcga gtcggcgctg acgcacgcca gaaaactgct gagcgacggc
      841 ggcgttctcg ccgtcaaggg catcggcgga taccacctgg cctgtgacgc aggcgacgag
      901 acggcggtca ccgagttgcg gtcgcgaaag cgccgcggcg acaagccctt cgccgtcatg
      961 gtgcccgacc tcgacaccgc acaccgcgtc gcggacatcg acgatgcggc acagcgactg
     1021 ctctgcggtc cgcagcgccc catcgtgctg atgccccgtc tcgcagccgc acccgtcgcc
     1081 acctcggtcg caccgcacaa cccagacctc ggcgtgatgc tggcttacac cccgttgcac
     1141 gcactgttgt tcggactgtc cggagacgcg ggcggcccgc aggtgctcgt gatgacatcc
     1201 ggcaatctcg gcggcgaacc gatctgcttc accgacgagg atgcgctgga acggctttcg
     1261 cacctcgcgg acggctggct gatgcacgac cgtgccatcc tggtgccgtg cgacgactcc
     1321 gtggtgcgtg tggtcgacgg caatcaattg ccgatccggc gctcccgcgg ctatgcgccg
     1381 ctgccggtgg cacttccggt ggcggtgccg ccgacgctgg ccgtcggtgg tgatctcaag
     1441 aacacgtttg cggtcgccga gcacaagtac gcctggctca gccagcacat cggcgacatg
     1501 gacgaccttg ccacgctggc ggctttcgac tcggccgaac agcatctcga ggcgctgacc
     1561 ggggtcagcc cggagatcct tgtcgcggat tcgcatccgg gctatcgctc taccgcgtgg
     1621 gcacaacgcc atgccggcgg gcgcccggtc cgctctgttc agcatcatca cgcacatatc
     1681 gccgcggtca tggccgaaca cgggttggac gggtcacagc gcgtgctcgg gttcgcgttc
     1741 gacggaaccg gatacggtcc cgaccgagcg atctggggtg gcgaggtgct gctcgccgat
     1801 tacaagggct atcagcggct ggcgcagctg aaatacgttc cgctggcagg tggtgatgtc
     1861 agcgtgttgc gtccgtaccg gatggccctg tcgcacctgt gggcggccgg cttgggctgg
     1921 gacgaggacc tggcgccggt gcgggcgtgt ccacccgagg agcgtcgcgc actgcggcac
     1981 cagctcgaga ccgggctcgg atgcgctccg acctccagca tgggccggtt gttcgacgcg
     2041 gtgtcgtccc tggcagatgt ccgacacgtg gtggcatatg aggcgcaggc cgcgatcgag
     2101 ctggagggct tgtcgcgcaa cgccgatggg caaggtaggc cctatgcatt cgctgtcgac
     2161 gccggccggc aaccggcggt gatcgaccca gggcccgtgc tcgccgcgat catcgacgat
     2221 cagcgcgccg gtacaccagc tggtgtgatc ggggcgaggt tccactgcgc ggtcgccgac
     2281 ctggtcgtcg agctcgcagg aatggaggac acgggtggcc agccggtcgc attgtccggc
     2341 ggagtgttcc agaacgcgat gttgctgcgc ctggtgctgg atggcctgcg agccaagggt
     2401 ttccgggtga tcacccaccg ccatgtgccg cccaacgacg gcggtatcgc acttggccag
     2461 ctgctggtcg ggacggcggg ttgacacgat gaccgcccta cacgaacaga aactccccga
     2521 gggttacaca ttgttcgcgc gctacgcgtt cccgcccaac gagctcgggt actgcggtcc
     2581 ctcggattcc ggcgtgctgc tgcgcggcga caacccggcc ggagtggccg cccacgccaa
     2641 gcagttcgac ggcgcgtggc cgtatctgca cgccattgcc gacgcggcgg gcacacccga
     2701 tgcgttggac gccgacgtcg tgcgcagcta ctgggtcggt gggccgcacc tcgctcgggt
     2761 cgatccggcg gccctgctgg agaagctccg tcacgcattc aagggtcagg caacggggat
     2821 gcttttcgat ctcgcccacc cgcacggcac gctggcgcat cacagtttcc acgtcttcgt
     2881 ggtgtacccc tgggtgcggt tcctcgaccg cgacgcgccg accgcgctga aagtgctgca
     2941 ggactgccgg atccgctggg gagcagttga atccgtcgac gatgagcacg cggtgatcgc
     3001 gacccgtccg ctgatgttcg ccgacggcat cctggcgctc ggcgagccga gatccgagcg
     3061 ggtgcggtgg agcaaggacg gaatctcgct gatcgggcca cccgcacccg gcgacgtagt
     3121 gtcggcgcac tgggactggg cgtgcggcgt actcaccgac gacgagtgtg cggcactggg
     3181 cgaagcgacg cagacaacgc tcgatctcgt gaacaaagcc cgcaacgagg agcgaaaccg
     3241 atgacagtca ccacagaacc gggcggtggg ttcgtcgaca ccgctctgtc cgcagacttg
     3301 gcagccgctg cgctggacat ggcccgcagg tttcacgagg gcgccacact ttgggtgatc
     3361 tccccgcaat gggaaccgca cgcacatcac gtcgcggtcg agttcgtcca tccggtgatc
     3421 atgggtaaac gggcgctgcc gtcggtcgcg ctcgtcgagc ccgacccggt ggcccaggcc
     3481 cgggtcgccg cccagcccgg tgacctgctg atcgccgtgg cgtctgccga cgaacccgcg
     3541 gtgatagagg cgatgcgacg agcaccggca tggggcgcgc acaccttgtg gatcgggtcg
     3601 ggcccgcgtc caccggcggg agcggccaat cacatcctct gggtggactc cgacgatccg
     3661 atggtgcctg cgacgggacg cttcgtcctg atgtatcacc tgctgtggga actcacccac
     3721 gtctgtttcg aacaccccgg cctgctcaag cccccggtcg aggagtgcac cgacgaagtc
     3781 tgcgtgacgt gcagcgacga ggggcggctc ggcgaagtgg tgttcgagcc cgctgacccg
     3841 atgggttcgg cgctggtacg caccgcgacc ggcgaggaat gggtggacgt caccattttg
     3901 ggcgaagttg cgccgaacga tctggtgctg gtgcacgcgg ggacggccat cgcccgtctc
     3961 ggcgatgagg aggtgaaccg atgacgacgc acggcaagga aagcaccgaa agcacggcat
     4021 ttctgtatcc gttcatcgac tcggaggaaa ccgacgcagg cagtctcctc gacgacctct
     4081 ccgtatcggc aaggggcaaa gcttccgaaa gtgcacgcct gcaacaggag tcactggacg
     4141 aatatgcgcc ggcgctaacc gccgcgggcg tcgagatggc cgaacggttc ctgcgcggcg
     4201 gccgcctcta cacattgggc aacgggggca gctccaccga cgctgcgacg ctggcatcgt
     4261 tgttcagccg cccggccagg ggccgcgccg tagcggcatg gtcgctggcc gcggacgagg
     4321 cgatcgtgac ggccctcggc aacgacgtcg gcttcgacct catcttcaaa cgccagatca
     4381 tcgcccacgc gcgcgatcgc gatgtcgcca tcgcgttgtc gacctcgggc aactccgagg
     4441 acctgatgtc ggccgtcacc gaagccaaac agcgtgggct gctgacaatc gggttctccg
     4501 gccacgacgg cggccgcatg gcggtggccg aggacctcga ctactgctac acggtgcaca
     4561 gccagagcat ccaccggatc caggaaagcc atgccctgct gggtttccgg ctctggtcgg
     4621 ttgtgcagga acacatgtcc cgaccccgta acggagtgca cacttcatga gcccaaccga
     4681 cgcgatgact gccgccgaac gcgaagaccg cgtgctggaa cggatcgaca agttccgcca
     4741 gcgacgcccg cggctgctcg acgacgtggt gacgctcgca cacggcgccg gcgggaagtc
     4801 gtcggc
//
LOCUS       DASYLX010000172         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_227164,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000172 DASYLX010000000
VERSION     DASYLX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227164"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VGP27_04210"
     CDS             <1..1204
                     /locus_tag="VGP27_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7850555.1"
                     /translation="QLQEVGYETEYIEAGAPGRANVFTRLKGADSSRGALMLHGHLDV
                     VPAEASDWSVHPFSGALSDGYVWGRGAVDMKDMVGMMIAVARGFKRSGTVPPRDLVFA
                     FVSDEEAGGKYGCKWLVENRSDLFEGVTEAVGEVGGFSLTIPRREGGERTLYLIETAE
                     KAMKWMRLTARGRAGHGSMVHDNNAVTAVAEATARLGRHQFPVVLTDAVEQFLQAVAE
                     ETGYTFDVDSPDLEGAIAKLGPIAKIVGATLRDTANPTMLKAGYKANVIPATAEAVVD
                     CRVLPGRLAAFEREVDEIIGPDVSREWITDLPAYETTFDGELLEAMNAAILAADPDAR
                     TVPYMLSGGTDAKHFATLGIRCFGFAPLKLPPELDFASLFHGVDERVPVDALTFGAQV
                     LEHFLLHC"
     gene            1229..1759
                     /locus_tag="VGP27_04215"
     CDS             1229..1759
                     /locus_tag="VGP27_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEV7850556.1"
                     /translation="MAFPYNTYDHLPELPTFTLTSESFSDGEAWSNDQVSGIMGAGGA
                     DVSPQLSWSGFPEETKSFAVTVYDPDAPTASGFWHWAVANLPASVTELPAGAGDGSEL
                     PGGAMTLANDAGQKRFIGAAPPEGHGVHRYYVVVHALGVEKLDLPEDATPAYLGFNLF
                     GQAIARAIIHGTYEQN"
     gene            complement(1856..>2416)
                     /gene="pyrD"
                     /locus_tag="VGP27_04220"
     CDS             complement(1856..>2416)
                     /gene="pyrD"
                     /locus_tag="VGP27_04220"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEV7850557.1"
                     /translation="PLAAYVVVNVSSPNTPGLRDLQAVESLRPILAAVREETTTPVLV
                     KIAPDLSDSDVDEIADLAVELGLAGIVATNTTVSRDGLATPGVDRLGDGGISGPPVAR
                     RSAEVLRRLYRRVGDRLVLISVGGIESADDAWDRITSGASLLQGYTGFIYGGGLWAKQ
                     IHDGVARRLHEGRFSSLADAVGSAVR"
BASE COUNT          407 a          817 c          813 g          379 t
ORIGIN      
        1 gcagctgcag gaggtcggct acgagacgga gtacatcgag gccggcgcac cgggcagggc
       61 gaatgtattc acccggctga agggggctga ctcgtcccgc ggcgccctga tgctgcatgg
      121 ccacctcgac gtggtgcccg ccgaggcgtc ggactggagc gtgcacccgt tctccggcgc
      181 cctctccgac ggctacgtct ggggccgcgg cgcagtcgac atgaaagaca tggtcggcat
      241 gatgatcgcg gtcgcccgcg gcttcaaacg ttccggcacc gtgccgccgc gcgacctggt
      301 gttcgcgttc gtctccgatg aggaggcagg cggcaaatac ggctgcaagt ggctcgtcga
      361 gaaccggtcc gacctgttcg agggtgtgac ggaggcggtc ggcgaggtcg gcgggttctc
      421 gctgacgatt ccgcgccgtg agggcggcga gcgcacgctc tacctgatcg aaaccgccga
      481 gaaggcgatg aagtggatgc ggctgaccgc gcgcggccgg gcgggccacg gatcgatggt
      541 gcacgacaac aacgcggtga ccgccgttgc cgaggcgacg gcccggttgg gacgccacca
      601 gttccctgtg gtgctgaccg atgcggtcga acagttcttg caggccgtcg cggaggagac
      661 cggctacacg ttcgacgtgg actcgcccga tctcgaaggc gctatcgcca agctcggtcc
      721 gatcgccaag atcgtcggcg cgacgctgcg ggacaccgcc aacccgacga tgctcaaggc
      781 gggttacaag gccaacgtga ttcccgcgac cgcggaggcg gtcgtcgact gccgcgtgct
      841 gcccggcagg cttgcggcat tcgaacgtga ggtcgacgag atcatcgggc cagatgtcag
      901 ccgcgagtgg atcaccgacc tgcccgccta cgagaccacc ttcgacggcg agctgctcga
      961 ggcgatgaac gcggcaatcc tggccgccga ccccgacgcc cgaacagtgc cttacatgct
     1021 gtcgggcggc accgacgcga aacacttcgc cacgcttgga attcggtgct tcgggttcgc
     1081 cccgttgaag ttgccgcccg agctcgattt cgcgtcgctg ttccatggcg tcgacgagcg
     1141 ggtacccgtt gacgcgttga cgtttggcgc ccaggttctc gagcacttcc tgctgcactg
     1201 ctgatcacac gatttgaaag gaccaccgat ggcattcccg tacaacacct acgaccacct
     1261 gccggagctt ccgacgttca cgctgacctc tgagtctttc agcgacggag aggcatggtc
     1321 caacgaccag gtcagcggca tcatgggcgc aggcggtgcg gacgtctccc cgcagttgag
     1381 ctggtcgggc ttccccgagg agacgaagag tttcgcggtg acggtctacg acccggatgc
     1441 accgaccgcg tcaggcttct ggcactgggc ggtggccaac ctgcccgcga gtgtcaccga
     1501 gctgccggcc ggtgccggcg acggcagcga gttgcccgga ggcgcaatga ctttggcgaa
     1561 cgacgccggc cagaagcggt tcatcggcgc cgcgccgccg gagggtcacg gcgtgcaccg
     1621 ctactacgtc gtcgtgcacg ccctcggcgt cgagaagctg gatctgcccg aggacgccac
     1681 ccccgcatac ctgggtttca acctgttcgg tcaggcgatc gcgagggcga tcattcacgg
     1741 cacctacgag cagaattaac cgcggcgcct tcaccccgcg agcgtgcgcg tctgctcgtc
     1801 aacacgccgt aatgtcgtga cactccgtgc acgctcggcg acccatcagc gacacctagc
     1861 gcaccgctga gccgacggcg tcggccagtg acgagaaccg gccctcgtgc aatcggcgcg
     1921 cgacaccgtc gtgaatctgc ttggcccata ggccgccgcc gtagatgaag ccggtgtagc
     1981 cctgaagcaa cgaggccccc gatgtgatgc ggtcccaggc gtcgtcggcc gattcgatgc
     2041 cgccgacact gatcaacacc agccggtcgc ccacccgccg gtacagccgc cgcagcacct
     2101 cggccgaccg ccgcgcaacc ggcggaccgg aaatcccgcc atcgccgagc cgatccaccc
     2161 cgggggtggc caggccgtcg cgggacaccg tcgtgttggt cgcgacgatg ccggccaggc
     2221 ccaattcgac ggccagatcg gcgatctcgt cgacatcgga atcggacagg tcgggcgcga
     2281 ttttcaccag caccggcgtc gtcgtctctt cacggaccgc ggcgagaatg ggtcgcagcg
     2341 actcgacggc ctggaggtcg cgcagccccg gcgtgttcgg cgagctgacg ttcacgacga
     2401 cgtatgccgc cagcgg
//
LOCUS       DASYLX010000173         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1324172,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000173 DASYLX010000000
VERSION     DASYLX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324172"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGP27_04225"
     CDS             <1..504
                     /locus_tag="VGP27_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850558.1"
                     /translation="VAVVTALRALGNDIPICVLSARDTVNDRIAGLEAGADDYLTKPF
                     DLGELVARLHALLRRTSQSNQSSDTMSVGPLTIDTARRLVFVDGERVDLTKREFDLLA
                     VLAENAGVVLSRQRLLELVWGYDFDVDTNVADVFISYLRRKLEREGLPRVIHTVRGIG
                     YVLRDET"
     gene            574..1920
                     /locus_tag="VGP27_04230"
     CDS             574..1920
                     /locus_tag="VGP27_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7850559.1"
                     /translation="MRRLRLARSASLRTRVALAAATAAAAVVAVFTILTSLVLANNDA
                     AQLDRRLDSIVEASMYPDQLSNPRQGVLTTGRMESTGQVVFQRGLQLPRLQPGTADVV
                     VNGVDYRVRTIPVDQQGGVLMSIGIRADSILLNRARIPFYVAVGLVTVLIAAGVGWLL
                     SGPAIRPLRRLTEHTRRLGKGTEEMPEVRGVREAEELSEAMTGMLSRLAAAQQATQNS
                     LQAAQDFAANAAHELRTPLTAMRADLDTLRLHDLPEEERAEVIMDLSRAQRRVEAIIT
                     ALGQLASGELAQAEDREVIDVTDLLDRVARENMRPGGSVQVVVEADEHLGTIWGWPSG
                     LRLAVDNLVRNAVAHGSASRVLLTAHRIDHSITITVDDNGGGLPAEEHHIVLGRFARG
                     STAAPGGSGLGLALVAQQAALHGGSIVLSESSLGGLRATLTVAASTEPQWHSNAQE"
     gene            complement(1911..2279)
                     /locus_tag="VGP27_04235"
     CDS             complement(1911..2279)
                     /locus_tag="VGP27_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEV7850560.1"
                     /translation="MKRTSALAALADVAGVLVFCTAGRRSHAEGLTVAGVAETAWPFL
                     VGTAVGWLLSRGWRRPTAVVPTGLAVWVCTVAIGMLLRKATSAGVAGSFVVVASVATA
                     VVLLGWRLVVAALRRRPHHS"
     gene            complement(2266..>2832)
                     /locus_tag="VGP27_04240"
     CDS             complement(2266..>2832)
                     /locus_tag="VGP27_04240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV7850561.1"
                     /translation="FAIDYIQLKPDGTMSTGDLTKVESYPYFGADIHAVADGPVVGAL
                     EGLPEQIPTKSPTDLKLEEYGGNHIVQDIGNGNYAFYAHLKTGSLKVKVGDQLSTGQV
                     IASLGNTGNSDAPHLHFHVMSTPDPLRSDGLPFVIKSFRLDNRVGSQDALDALLGGKP
                     APMQPGFTARDESDVSPLVLDVMTYETH"
BASE COUNT          478 a          916 c          975 g          463 t
ORIGIN      
        1 gtcgcggtcg tcactgcgct gcgggcactg ggtaacgaca tccccatctg cgtgctgtcc
       61 gcgcgcgaca ccgtcaacga ccggatcgcc ggcctcgagg ccggcgccga cgactatctg
      121 acgaagccct tcgatctcgg cgagctggtg gcacggctac acgccttgct gcgccgcacg
      181 agtcagtcca atcagtcgtc ggacaccatg tcggttgggc cgctgaccat cgacaccgcc
      241 cggcgactgg tgttcgtcga cggtgagcgg gtcgatctga ccaagcggga gttcgacctg
      301 ctggcggtgc tggccgagaa tgcgggcgtc gtgctcagcc gccagcgact gctggaactg
      361 gtgtggggat acgacttcga cgtggacacc aacgtggccg acgtattcat cagctacctg
      421 cggcgcaaac tcgaacgcga gggcttgcca cgggtgatcc acacggtgcg cgggatcggc
      481 tatgtgctgc gagacgaaac gtagtggagt cgagcatccc gcagagagac gaaacgaagt
      541 gaagtcgagc atcctgtacc gagacgaaac gtagtgcgcc gcctccgttt ggcgcgttcc
      601 gcttcgctac gcaccagggt ggcgctggcc gccgctaccg ccgccgccgc cgtcgtcgcg
      661 gtgttcacca ttctgacatc actggttctc gccaataacg atgcggcaca actggatcgg
      721 cggctggact ccattgtcga ggccagcatg taccccgacc agttgtcgaa tccccggcag
      781 ggagtgctga cgacaggccg gatggagtcg accggccagg tggtttttca gcgtgggctt
      841 caactgccgc gcctgcagcc gggcacggcc gacgtcgtcg tcaacggcgt ggactatcga
      901 gtgcgcacca ttcccgtgga ccagcaaggc ggcgtgctga tgtcgatcgg catcagggcc
      961 gacagcattc tgttgaaccg ggcccgcata ccgttctacg tcgcggtcgg cctcgtcacg
     1021 gtgttgatcg cggcgggggt gggatggctg ttgtcgggcc ccgcgatccg gccgctgcgg
     1081 cggctcaccg aacacaccag acggctcggc aagggtaccg aggaaatgcc ggaggtacgc
     1141 ggtgtccgcg aggccgaaga actgtccgag gcgatgacgg gaatgctcag ccggctcgcc
     1201 gccgcgcagc aggccaccca gaactccctg caggccgcac aggatttcgc ggcgaacgct
     1261 gcacacgagt tgcgcacacc gctgaccgcc atgcgtgccg acctggacac gctgcggctt
     1321 cacgacctac ctgaagagga gcgggccgag gtgatcatgg acctgtcgcg ggcgcagcgt
     1381 cgcgtcgagg cgatcatcac ggcgctcggt cagctcgcgt cgggtgagct ggcacaggcg
     1441 gaggaccgcg aagtcatcga cgtgaccgac ctgctcgacc gggtggcgcg ggagaacatg
     1501 cgcccgggtg ggtcggtgca ggtcgtcgtc gaggccgacg agcacctcgg caccatctgg
     1561 ggctggccga gcggcctgcg ccttgcagtg gacaacctgg tgcgcaacgc cgtggcacac
     1621 ggatcggcgt ccagggtgct gctgacagct caccggattg atcactcgat aacgatcacg
     1681 gtcgacgaca acggtggcgg tctgccggcc gaggaacatc acatcgtgct cggccgcttc
     1741 gcacgcggat ccaccgccgc gcccggaggt tcgggactcg gccttgccct ggtggctcag
     1801 caggcggcac tgcacggcgg cagcatcgtg ctgtccgaga gttcactggg cggcctgcgt
     1861 gccaccttga cggtggcggc ttccactgag ccgcaatggc attcgaacgc tcaggaatga
     1921 tgcggcctgc gccgtagggc tgcgacgacc aggcgccagc cgagcaggac caccgccgtc
     1981 gcgacggacg ccaccacgac gaagctcccc gcgacgcccg cggaagtggc cttgcgcaac
     2041 agcattccga tcgcgaccgt gcacacccag acggccaatc cggtcggaac gaccgctgtc
     2101 ggacggcgcc agccgcggct gagcagccag ccgaccgcgg tgcccaccag gaacggccac
     2161 gcggtctcgg ccacacctgc gacggtcaag ccctcggcgt ggctgcggcg tccggccgtg
     2221 cagaacacca gcacaccggc gacgtcggcg agcgcggcca gtgcgctagt gcgtttcata
     2281 ggtcatcacg tcgagcacca gggggctgac gtcggattcg tcgcgggcgg tgaaccccgg
     2341 ctgcatcggt gcgggtttgc cgcccaataa cgcgtcaagg gcgtcctggg agccgacgcg
     2401 gttgtcgaga cgaaacgact tgatcacgaa cggcagcccg tcggaccgca gcggatccgg
     2461 cgtgctcatc acgtggaaat gtagatgtgg cgcatccgaa ttgccggtgt tgcccagtga
     2521 cgcgatgacc tgtccggtgc tgagttgatc gccgaccttg accttcaggc tgccggtctt
     2581 caggtgcgcg tagaacgcgt agttgccgtt gccgatgtcc tgcacgatgt ggttgccgcc
     2641 gtactcctcg agcttgagat cggtcgggct cttcgtcgga atctgctcgg gcaggccttc
     2701 gagcgctccg acgacgggac cgtcggcgac cgcatggatg tcggcgccga agtagggata
     2761 gctttcgacc ttggtgaggt cgccggtgga catcgtcccg tcgggcttca gctggatgta
     2821 gtcgatggcg aa
//
LOCUS       DASYLX010000174         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3079702,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000174 DASYLX010000000
VERSION     DASYLX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079702"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGP27_04245"
     CDS             <1..373
                     /locus_tag="VGP27_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEV7850562.1"
                     /translation="DSPLGRLLRAAATGGLADHPPLHWRDGAAVTVVLAAENYPGRPR
                     VGDVIAGSEADGVLHAGTARRDDGAVVSSGGRVLSVVGAGPDLAAARDDAYAVITSVR
                     LPGSHFRSDIGLAAAEGRITV"
     gene            complement(378..1259)
                     /locus_tag="VGP27_04250"
     CDS             complement(378..1259)
                     /locus_tag="VGP27_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850563.1"
                     /translation="MMRLGASAAAGAVGAFALGSLREPQPSVAGPPVEMTSAGTPLAP
                     LAPPAPLEPLPAAAQTYVSGSFVSAARGGIDTNWAIARPPGQTQPLRSVIALHGKGGD
                     AAQVMAGGVERGLAQAVAAGLPPFALVAVDGGGGYWHKRASGEDSGAMVLDELIPMLG
                     SQGLDTSRVGFLGWSMGGYGALLLGARLGPARTAAICAVSPALWTSSGATAAGAFDGA
                     DDYAANSVWGLPALGSIPIRIDCGNSDPFYSATQQFIAQLPNPPAGGFSPGGHDGGFW
                     SSQLPAELEWMAPLLTA"
     gene            1503..>1768
                     /locus_tag="VGP27_04255"
     CDS             1503..>1768
                     /locus_tag="VGP27_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850564.1"
                     /translation="MQGVTAAVTPKGVRRQYALVSAAAELLCEGGFDAVRHRAVARRA
                     GLPLAATTYYFSSLDDLILKAVEHVGALEAQQLRERVAGLSRRRR"
BASE COUNT          261 a          634 c          609 g          264 t
ORIGIN      
        1 cgattcgccg ctcggccggc tgctgcgcgc cgcggccacc ggcggcctcg ccgaccaccc
       61 gccgctgcac tggcgcgacg gcgccgcggt gaccgtcgtg ctggccgccg agaactatcc
      121 gggacggccg cgggtgggcg acgtcatcgc cggctcggaa gccgacggag tgttgcacgc
      181 aggcaccgca cgacgcgacg acggcgccgt cgtctcatcg ggaggccgag tgctctcagt
      241 cgtcggcgcg ggaccggacc tggcggcagc ccgcgacgac gcctatgccg tgatcacatc
      301 ggttcggttg ccaggcagtc actttcgcag cgatatcgga cttgccgcgg ctgaaggccg
      361 gatcaccgtc taggtgacta cgccgtcagc agcggcgcca tccactcgag ttccgcaggc
      421 agctgcgaac tccagaaccc gccgtcgtgg ccgcccggtg agaagccgcc cgcaggcggg
      481 ttcggcagct gcgcgatgaa ctgctgggtg gccgaataga acggatcgct gttgccgcag
      541 tcgatccgga tcggtatcga tccgagggcg ggcagacccc acacgctgtt ggccgcgtag
      601 tcgtctgcgc cgtcgaacgc ccccgcagcg gtcgcacccg acgacgtcca caacgccggg
      661 ctcaccgcac agatcgcggc ggtacgcgcc gggccgagcc gggcccccag cagcaacgcg
      721 ccgtatccgc ccatcgacca gcccaggaac cccactcgcg aggtgtcaag gccctgcgag
      781 ccgagcatcg ggatcaactc gtcgagcacc atcgcgccgg aatcctcgcc cgatgcgcgc
      841 ttgtgccagt aaccgccgcc gccgtcgacg gcgaccagcg caaagggcgg caggcccgcc
      901 gcgaccgctt gcgccaggcc tcgctcgaca ccgcccgcca tcacctgcgc cgcatcgcca
      961 cccttaccgt gcagcgcgat gacggatcgc agcggttgag tctggcccgg cggccgcgcg
     1021 atcgcccagt tggtgtcgat gccaccgcgt gcggccgata cgaatgaccc ggacacatac
     1081 gtctgcgccg ccgcaggcaa cggctccagc ggggcgggcg gtgccaacgg tgccagcggg
     1141 gtgcctgcgc tcgtcatctc gacgggtgga cccgcgacgg atggctgcgg ttcgcgtagt
     1201 gagccgagcg cgaaggcgcc gacggcccct gccgcagcac tggcaccgag gcgcatcatc
     1261 gcccggcggc tcagttgatt gcctcgctcg tccctcgtcc cctgcccttc gggcgtgggc
     1321 ccagcgatcc tcactcgtgc tcgcctcggt ggctccggca tgcgggccat catgccattg
     1381 gcctccggcg catcgtcggg cggcgccggc ggaatgcatc caaatcgctt ggcgggccat
     1441 cttcctgagt aaaggcttag ctttggccga aagagtcatt tcgacctcgg tcgactggca
     1501 gcatgcaagg cgtgacggca gcggtgactc ccaagggggt gcgcaggcag tacgcgttag
     1561 taagcgcggc tgccgagctg ttgtgcgaag gcggtttcga cgccgtgcgt catcgtgccg
     1621 tggcccggcg tgccgggctg ccgttggcgg cgacgaccta ttacttctcg tccctcgacg
     1681 atctgatcct caaagccgta gagcacgtcg gcgcactcga ggcgcagcaa ctgcgtgagc
     1741 gggtggcggg gttgtcgcgg cgtcgtcg
//
LOCUS       DASYLX010000175         6269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_995030,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000175 DASYLX010000000
VERSION     DASYLX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995030"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1054)
                     /locus_tag="VGP27_04260"
     CDS             complement(143..1054)
                     /locus_tag="VGP27_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEV7850565.1"
                     /translation="MPRSTQMVPFRSHQTWVQITAPEDPRPGAQPLFVLHGGPGMAHN
                     YVRNIAELADETGRTVIHYDQIGCGKSTHLPDAPADFWTPELFVEEFHTVREALGIDE
                     YHLLGQSWGGMLSAEIAVLQPPGLTSVAICNSPASMELWMAGAAELRAALPPDVQDAL
                     TRHEEAGTVTDPEYLAATDEFYRRHLCRVVPTPQDLVDTIEQMEAEPTVYHTMNGPNE
                     FHVLGTLRGWSIIDRLPQITAPTLVIAGEFDEATPQAWQPYVDQIAGADSHVFPATSH
                     CSHLEKPEEFRAVVAAFLARHDATARV"
     gene            1141..1950
                     /locus_tag="VGP27_04265"
     CDS             1141..1950
                     /locus_tag="VGP27_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7850566.1"
                     /translation="MAHDSAPGGLSHQQRDTPTRVDEITDRLITAIAIGEYLPGARLP
                     VERDLAASLRAGRMTVRAALARLLDRGLIETRRGRGGGSYVRDQWPASSTDAVGRTLR
                     ARLGELRDRCDAICRLQGTVSRAAAESRTEHDISAIQARFEAYRTAKSGLDAQQADSR
                     LHLAIMDAAHNDVLKQVLLDVKATVSIGAPAHLWGEPSTMRTMELRALHEHEQLVGAI
                     ADGRADDADDLARHHGLIDFELITRAMERAGVFTHPPSSAAHRSAWRRVVS"
     gene            complement(1943..2530)
                     /locus_tag="VGP27_04270"
     CDS             complement(1943..2530)
                     /locus_tag="VGP27_04270"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV7850567.1"
                     /translation="MGVNTVAARLLDVMERDILPMTERGVAAGNKVFGAALLRKSDLS
                     VVIAGTNAETDNPLLHGEISTLNHFYERSARPSTQDLIFLSTHEPCPLCLSAITWCGF
                     DNFYYFFTYEDSRDAFGIPHDLKILKEVFGVEDGAYRRKNAFWTAYSIGALVETEAEP
                     LRTRLRAQDKRIRDVYAALSEQYQSSKADNTIPFN"
     gene            2614..3366
                     /gene="thyX"
                     /locus_tag="VGP27_04275"
     CDS             2614..3366
                     /gene="thyX"
                     /locus_tag="VGP27_04275"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV7850568.1"
                     /translation="MAETAALRVQLIAKTEFLAPPDVPWSTDADGGPALVEFAGRACY
                     QSWSKPNPRTATNAGYLRHIIDVGHFSVLEHATVSFYITGISRSCTHELIRHRHFSYS
                     QLSQRYVPEHDSQVVVPPDMEDDQELQEIFTAAADASRATYSELLARLEAKFADQPSA
                     ALRRKQARQAARAVLPNATETRIVVTGNYRAWRHFIAMRASEQADGEIRRLAIACLRQ
                     LVDVAPQAFSDFEIATLADGTEVATSPLATEA"
     gene            3392..4294
                     /gene="dapA"
                     /locus_tag="VGP27_04280"
     CDS             3392..4294
                     /gene="dapA"
                     /locus_tag="VGP27_04280"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV7850569.1"
                     /translation="MSTSGFDVQARLGTVLTAMVTPFKPDGSLDTAAAARLAAHLVDA
                     GCDGLVLSGTTGESPTTTDDEKIALLRAVLDAVGDRARIITGAGTYDTAHSIHLAKAC
                     EAEGAHGLLVVTPYYSRPSQAGLTAHFTAIADAVSVPILLYDIPIRSGIPIEWDTIRA
                     LAGHPNIAGIKDAKGDLNGGGQIIAETGLAYYSGDDALNLPWLAMGAVGFVSVWGHVA
                     ASQLREMLSAFSSGDVATARKINVTLAPLSEAQSRLGGVTMSKEGLRLQGIDVGDPRL
                     PQVPATPDQIEALAADMRAAAVLR"
     gene            4294..5970
                     /locus_tag="VGP27_04285"
     CDS             4294..5970
                     /locus_tag="VGP27_04285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV7850570.1"
                     /translation="MDTDLKPPGPLVPGGLRVTALGGISEIGRNMTVFEHLGRLLIVD
                     CGVLFPGHDEPGVDLILPDLRLVEDRLDDVEALVLTHAHEDHIGAIPFLLKLRPDIPV
                     VGSKFTLALVAEKCREHRLKPSFVEVAAGQRTTHGVFECQYFHVNHSIPGCMAIAIHT
                     GAGTVLHTGDIKLDQLPLDDKPTDLPGMSRLGDAGVDLFLCDSTNSEIPGVGPSESEV
                     GPTLHRLIRGADGRVIVACFASNVDRVQQIIDAAVSLGRRVSFVGRSMVRNMGIAREL
                     GYLRVDEDDLVDIGAAEMMAPERVVLITTGTQGEPMSALSRMSRGEHRSITLTRGDLI
                     VLSSSLIPGNEEAVYGVIDSLAKIGARVVTNQQARVHVTGHAYSGELLFLYNGVRPRN
                     VMPVHGTWRMLRANAELATRSGVDPAGIMLAENGVSVDLVAGKASIAGAVPTGKMFVD
                     GSITGDVGESTLGERLTLSSGFVAVTVVVHRGTGRAAAPAHLHARGFSEDPKALEPVA
                     VKVEEELEILAGDHITDVTRIAQAVRRTVGKWVGETYRRQPMIVPTIIEI"
     gene            complement(5987..>6269)
                     /locus_tag="VGP27_04290"
     CDS             complement(5987..>6269)
                     /locus_tag="VGP27_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850571.1"
                     /translation="RAYANHLAPQRIRVNSIHPAAVSTPMINNEFTRQWLDRITRESD
                     APVDFGMALPVQFVTAEDIANAVAWLVSDEAWYITGVTLPVDAGYANKR"
BASE COUNT         1064 a         2037 c         2103 g         1065 t
ORIGIN      
        1 ccacggcgcg atcggcacca tgaagatcag gccgtagatg agcaggtcga cggtcgcgac
       61 cgagcggtgc aattcctgtg tgtagccaaa ggattcgagg ctacgttgac cttccatgac
      121 tgtcctgtcg ggtcgaggtg ggctagacgc gggctgtcgc gtcgtgacgg gcaaggaacg
      181 cggcgacgac ggcgcgaaac tcctctggct tctccaggtg cgagcagtga ctggtggcgg
      241 ggaaaacgtg gctatccgca cccgcgatct gatcgacata cggctgccag gcctgcggag
      301 tcgcctcgtc gaactcgccg gcgatgacca gcgtgggcgc ggtgatctga ggcagccggt
      361 cgatgatgct ccacccgcgc agtgtgccga gtacgtgaaa ctcgttgggg ccgttcatcg
      421 tgtggtatac cgtcggctcg gcttccatct gctctatggt gtcgacgagg tcttgcggtg
      481 tcggcaccac ccggcacaga tgccgccggt agaactcgtc ggtcgccgcc agatactccg
      541 gatcggtcac agtgcccgct tcctcgtgac gggtcagcgc gtcctgtacg tcggggggca
      601 aggcagcccg caactcggcg gcgccggcca tccagagttc catcgatgcc ggcgagttgc
      661 agatcgccac cgacgtcagc cctggtggtt gcaggacggc gatctcggcg ctcagcatgc
      721 cgccccagga ctggccgagc aggtggtact cgtcgatgcc cagagcttcg cggaccgtgt
      781 ggaactcctc gacgaacagc tcgggcgtcc agaagtccgc gggtgcatcg ggcaggtgcg
      841 tgcttttgcc acacccgatc tggtcgtagt ggatgacggt gcggccggtt tcgtcggcaa
      901 gttcggcgat gttgcggacg tagttgtgcg ccatgccagg tccgccgtgc aggacgaaca
      961 gcggctgcgc gccgggcctg gggtcctccg gtgcggtgat ctggacccag gtctggtggc
     1021 tgcggaacgg gaccatctgg gtgctacgag gcatggagtt cagcatggac cggtaaaggt
     1081 ctcatgacaa gaccattgat cgggatttaa cattcaaggc gacaacgaag gaatgaccgc
     1141 atggcgcacg acagcgcacc gggaggcttg tcacaccagc agcgtgacac gcccacccgc
     1201 gtcgacgaga tcaccgatcg gctgatcacc gcgatagcga tcggggagta cctgcccggg
     1261 gcgcggttgc cggtcgaacg cgatctcgct gcgtcgctgc gggcgggtcg catgacggtg
     1321 cgcgctgcgc tggcccgcct tctcgatcgg ggactgatcg aaacacgacg gggacgcggc
     1381 ggcgggtcgt acgtccggga ccaatggccg gcctcgtcca ccgatgcggt cggccgcacg
     1441 ctgcgggcgc gactgggcga actacgagac cgctgcgacg cgatctgccg gctgcagggc
     1501 accgtcagcc gggccgccgc ggagtcgcgt actgaacacg acatatccgc gattcaagcc
     1561 aggtttgagg cgtaccggac agcaaagagc ggcctcgacg cacagcaggc cgacagccgg
     1621 ctgcacctgg cgatcatgga cgcggcccac aacgatgttc tcaagcaggt gctgctcgac
     1681 gtgaaagcca cggtcagtat cggcgcgccg gcccaccttt ggggtgagcc gtccaccatg
     1741 cgcaccatgg agctacgcgc tcttcacgaa catgaacagc tggtgggtgc catcgccgac
     1801 ggccgcgccg acgatgccga cgatcttgcg cgtcatcacg gcctcatcga cttcgaactc
     1861 atcacgaggg cgatggagcg cgccggggtg ttcacccacc caccgagttc cgccgcgcat
     1921 cggagtgctt ggcgacgagt ggtcagttga acggaatggt gttgtcggcc ttggagctct
     1981 gatactgctc cgacagtgcc gcgtagacat cacggatccg cttgtcctgc gcgcgcagcc
     2041 gggtgcggag cggctcggcc tcggtctcga ccagtgcacc gatcgagtac gcggtccaga
     2101 acgcgttctt gcgccggtac gcaccgtcct cgacgccgaa gacctccttg aggatcttca
     2161 ggtcgtgtgg aatgccgaat gcgtcgcggg aatcctcgta ggtgaagaag tagtagaagt
     2221 tgtcgaatcc gcaccaggtg atagcagaca ggcacagcgg gcagggttcg tgggtggaca
     2281 ggaaaatcaa gtcctgcgtc gacggccggg cggaccgctc atagaaatgg ttcagtgtgc
     2341 tgatttcgcc gtgcaacagc ggattgtcgg tttctgcgtt ggtgccggcg atcaccacag
     2401 ataagtcgga cttgcgcaac agggccgcgc cgaacacctt gttgcctgcg gcgacaccgc
     2461 gttcggtcat cggcaggatg tcgcgctcca tcacgtccag aagccgtgcc gcgacggtgt
     2521 tgacccccat cgctgcagcg tagcggcgca agaccgccgt tccggtatgc aagccgcaac
     2581 gctttcgtaa cggtgctcgc gttagggtga gccgtggccg agaccgcggc gctgcgcgtg
     2641 cagttgatcg ccaagaccga gttcttggct ccccccgacg tgccctggag caccgacgcc
     2701 gacggcggac ccgcgctggt ggagttcgcc ggccgggcgt gttatcagag ctggtccaag
     2761 cccaacccgc ggaccgcgac caatgccggc tacctgcggc atatcatcga cgtcgggcac
     2821 ttctcggtgc tcgagcacgc gacggtgtcg ttctacatca ccgggatctc gcggtcgtgc
     2881 acgcacgagc tgatccgcca ccgccatttc tcgtactcgc agctgtctca gcggtatgtg
     2941 cccgagcatg attcgcaggt ggtcgtgccc cccgacatgg aggacgacca ggaactgcag
     3001 gagatcttca ccgcggccgc cgacgccagc cgtgccacct actcggagct gctggccagg
     3061 ctggaggcca agttcgccga ccagcccagc gccgccctgc ggcgcaaaca ggcccgtcag
     3121 gcggcgcgcg cggtgttgcc caacgcaacc gagacccgca tcgtcgtgac cggcaactat
     3181 cgggcctggc ggcacttcat cgcgatgcgg gccagcgaac aagccgacgg ggagatccgc
     3241 aggctcgcga tcgcttgcct gcgccagctc gtcgacgtcg ccccccaggc cttctccgac
     3301 ttcgagatcg ccacgctggc cgacggcacc gaggtcgcca cctccccgtt ggcgactgaa
     3361 gcctgaccat cagcgggtaa tcttggtggc cgtgagcacc agcggattcg acgtgcaggc
     3421 ccggttgggc accgtgctga ccgcgatggt gacgccgttc aagcccgacg gctcgctcga
     3481 caccgccgcc gccgcgcgct tggccgccca cctggtcgac gcgggctgcg acggcctggt
     3541 gttatccggc accaccggtg agtcgcccac gaccaccgac gacgagaaga tcgcgcttct
     3601 gcgcgcggtg ctggacgccg tcggcgaccg cgcccgcatc atcaccggcg ccggaaccta
     3661 cgacaccgcg cacagcatcc acttggcgaa ggcatgcgag gcagaggggg cgcacggact
     3721 gctcgtcgtc accccgtact actcgcggcc gtcgcaggcc ggattgaccg ctcacttcac
     3781 ggcgatcgcc gatgcggtgt cggtgccgat ccttctctac gacattccga tcaggtcggg
     3841 catcccgatc gaatgggaca ccatacgagc gctggccggg catccgaaca tcgccggaat
     3901 caaggacgcc aagggcgatc tcaacggcgg cgggcagatc atcgccgaaa ccgggctggc
     3961 gtactactcc ggcgacgacg ccctaaacct gccgtggctc gcgatgggtg cggtcgggtt
     4021 cgtcagtgtc tggggacacg tcgccgccag ccagctgcga gagatgttgt cggcgttctc
     4081 ttccggcgac gtcgccaccg cccgcaagat caacgtgacg ctcgcgccgc tgagtgaagc
     4141 gcagtcacgg ctcgggggcg tcacgatgtc caaggagggc ctgcggctgc agggcatcga
     4201 tgtcggtgat ccgcgactgc cgcaggtgcc tgcgacaccg gaccagatcg aggcgctggc
     4261 ggcggatatg cgcgccgcgg cggtacttcg atagtggata ccgatctcaa accaccggga
     4321 cctctggtcc caggaggtct gcgggtaacg gcacttggcg gcatcagcga aatcggtcgc
     4381 aacatgaccg ttttcgagca tctcggccga ctcctgatcg tcgactgcgg ggtgctcttc
     4441 cccggtcatg acgagcccgg cgtcgacctg atcctgcccg acctgcggct cgtcgaggat
     4501 cgtcttgatg acgtcgaggc gctcgtgctg acgcacgcac acgaagacca catcggtgcc
     4561 attccgttcc tgctcaagct tcgccccgac ataccggtgg tcggctcgaa attcacactc
     4621 gcgctggtgg cggagaagtg tcgcgaacat cgactcaagc ccagtttcgt cgaagtggcg
     4681 gcggggcagc ggacaacgca cggcgtgttc gagtgccagt actttcacgt caaccactcg
     4741 atccccggtt gcatggcgat cgcgatccac accggcgcgg gcaccgtgct gcacaccggc
     4801 gacatcaaac tcgaccagtt gcccctcgac gacaaaccga ccgaccttcc tggcatgtcg
     4861 aggctcgggg acgccggggt cgacctgttc ctgtgcgact ccacgaactc cgagatcccc
     4921 ggtgtcggac cgtcggagag cgaggtcggc ccgacgctgc accggctgat ccgcggcgcc
     4981 gacggccggg tcatcgtcgc gtgcttcgcc tccaatgtcg atcgcgtgca acagatcatc
     5041 gatgccgcgg tgtcgctcgg ccggcgggtg tcgttcgtgg ggcggtcgat ggtgcgcaat
     5101 atggggatcg cccgcgagtt gggctaccta cgcgtcgacg aggacgacct ggtcgacatc
     5161 ggcgcggccg agatgatggc gcccgaacgg gtcgtgctga tcaccaccgg aacccagggt
     5221 gagccgatgt ctgcgctgtc gcggatgtcg cgtggtgagc atcgcagcat cacgctgacg
     5281 cgcggcgacc tgatcgtgct gtcgtcgtcg ctgatccccg gcaacgagga agcggtctac
     5341 ggtgtgatcg actcgctggc caagatcggc gctcgcgtcg tcaccaatca acaagcgcgc
     5401 gtacatgtca cgggtcatgc ctattccggt gagctgctgt tcctctacaa cggcgtgcgg
     5461 ccacgcaacg tgatgccggt gcacggaacg tggcgaatgc tgcgggccaa tgcggagctg
     5521 gccacccgct ccggcgtcga ccccgcgggg atcatgctgg cggagaacgg tgtcagcgtc
     5581 gacctggttg cgggcaaggc cagcatcgcc ggtgccgtgc cgaccggaaa gatgttcgtc
     5641 gacgggtcga tcaccggcga cgtcggcgag tccactctgg gtgaacggct tacgctgtct
     5701 tcgggtttcg tcgcggtcac ggtcgtcgtg caccgcggca ccggacgtgc tgccgcaccc
     5761 gcgcacctgc acgcacgggg cttctccgaa gaccccaagg cgctggaacc ggtcgccgtg
     5821 aaggtggagg aggagctgga aatccttgcc ggcgatcaca tcaccgacgt cacccggatc
     5881 gcccaggcgg tgcggcggac ggtcggcaaa tgggtggggg agacctaccg ccgtcagccg
     5941 atgatcgtgc cgacgatcat cgagatctaa ccggccggca cgggcgtcag cgcttgttgg
     6001 cgtatcccgc gtcgacgggc aacgtcaccc cggtgatgta ccacgcctcg tcggacacca
     6061 gccatgccac cgcgttcgcg atgtcctcgg cggtgacgaa ctgcaccggc agtgccatgc
     6121 cgaaatccac cggtgcgtcg gactcgcggg tgatgcgatc cagccactgc ctggtgaact
     6181 cgttgttgat catcggtgtg ctcaccgcgg ccggatgaat tgagttcacc cggatgcgtt
     6241 gtggcgcaag atggttggcg tacgctcgc
//
LOCUS       DASYLX010000176         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1653892,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000176 DASYLX010000000
VERSION     DASYLX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653892"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGP27_04295"
     CDS             <1..595
                     /locus_tag="VGP27_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7850572.1"
                     /translation="RTVRASELLDYTNYTGDYEREMGIGRPAEKKAMFETPDILVFAW
                     GGTVPMIAHAAGIMLDEITTTYDKWVTPTQRKSAKGVIPAGNVAAVRFTINGVYQGET
                     RIQLEHVNRIGDDAAPDWPTGNQNDVYRVDIEGTPSIFQETAFRFTDGSGRDAAAAGC
                     LATGMRALNAVPAVNDLSPGWVTPLDLPLIAGMGTVR"
     gene            655..2409
                     /locus_tag="VGP27_04300"
     CDS             655..2409
                     /locus_tag="VGP27_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7850573.1"
                     /translation="MADGVGLSVGATSLTAVAVGRAAVTRSAVVTLFPNRPLEVGVAG
                     ENPRLNADDRGVVITDFVDRVGDPVGIVAPDGSTHRGEILLAESLRALLYEVTNGRGA
                     GDPVVVTHPAHWRPASVEALRGALSQTREFAATALVSDATAAVTALRDDPGLPTSGVI
                     ALCDFGGSGTSITLVDAGAGQPVGETVRYADLSGDLIDTAVLTQVLEGLPDAGSADVT
                     GTSAIGPLSRLRTQCRAAKERLSTTAATSLVAELPGQRGEIRLTRNELDDSIRQPLTD
                     FAGALQETLDRNGVRALAAVASAGGGARIPLITTTLSEHFGVPVVTTAQPELTAAIGG
                     GLNAARGTAEEGATALAEALSPPTAAAAAVAATQMASEVHPDDMGAGPSSGLAWSEAD
                     DVPDLVASTDQYDYAGAAPTDGMAGPRPPMQFEPETDQYEAERRRSPFALLIAGLVVV
                     LLAIALAVWFVLRNNESTPAPSSTTTTATTTAAVPPPASEAPPPASEVPPSEEPPPVT
                     QTITPGPETVTQTQEPPPPPPTSEAPPPPTSEAPPPPASPEPPPSQPPSSPPRLIPNY
                     PTIPGLPFVPAPVIPPAP"
     gene            2420..>3561
                     /locus_tag="VGP27_04305"
     CDS             2420..>3561
                     /locus_tag="VGP27_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850574.1"
                     /translation="MTQTDGLLFNPNTYDPQQFDAETRRLLRATIDFFEGQGKKRLLD
                     DDLKVVWPADFVDFVKRERLFATFLTPSEFAGADEGSTTSTGVSNKRWDAARNAALSE
                     IFGFYGLTYWYTEQVTILGLGPIWQSENKDAKLKAADDLDAGEVMAFGLSEREHGADV
                     YNTDMVLTPASEEDRANGILYRATGEKYYIGNGNVASMVSVFGRRDDAEGQDAYVFFA
                     ADSRHENYHLVDNVVHMQIYVSTFRLENYPVRAEDILHTGVEAFAAALNTVNIGKFNL
                     CTCSIGMVEHAFYEAITHAQNRILYGNPVTDFAHVRANFVDSYARIVAMKMFSQRAVD
                     YLRSASLDDRRYLLFNPVTKAKVTMEGENIMRALHDVVAAKGYEKNT"
BASE COUNT          623 a         1254 c         1159 g          525 t
ORIGIN      
        1 tcggacggta cgggcttcgg agttgctgga ctacaccaac tacaccgggg actacgagcg
       61 ggagatgggc atcggcaggc cggccgagaa gaaggcgatg ttcgaaaccc ctgacatcct
      121 cgtattcgcc tggggcggaa cggttcccat gatcgcacat gctgcgggca tcatgcttga
      181 cgagatcacc accacctacg acaaatgggt cactcccacc caacgcaaat cagccaaggg
      241 cgtcattccg gcgggcaacg tcgccgccgt ccggttcacc atcaacggcg tctaccaggg
      301 tgaaacccga atccagctgg aacacgtcaa ccgcatcggc gacgacgccg cccctgactg
      361 gccgaccggc aaccagaacg acgtgtaccg cgtcgacatc gagggcaccc caagtatttt
      421 ccaggagacg gcgtttcgat tcaccgacgg ctccggccgg gacgccgcgg ccgcgggctg
      481 cctggcgaca ggaatgcggg cgctcaacgc ggtacccgct gtcaacgacc tgtcgccggg
      541 ctgggtgacc cctttggacc tgccgctgat cgccgggatg ggcacggtcc gctgagtaac
      601 gcacgccagc ccgtcctgga tgaacgatag ggttggccgc gagattggat tgacatggct
      661 gatggagtag ggctgtcggt tggtgccacc agcctgacgg cggtagcggt gggccgggcc
      721 gcggtgacgc ggtccgccgt ggtgaccctt tttccgaacc ggccgctcga ggtcggcgtg
      781 gccggcgaga atcccaggtt gaacgccgac gaccgcggcg tggtcatcac cgatttcgtg
      841 gaccgcgtcg gcgacccggt gggcatcgtg gcacccgacg gcagcaccca ccgcggtgag
      901 attctgctcg ccgagtcgct gcgcgcgctt ctctacgagg tgaccaacgg ccgaggggcc
      961 ggggaccccg tcgtcgtgac ccaccccgcg cattggcgcc ctgccagtgt ggaggcgctg
     1021 cgcggggcgc tttcccagac gcgcgaattc gccgcgacgg cgttggtctc cgacgcgacg
     1081 gcggctgtca cagcgttgcg cgacgatccc ggcctgccga ccagcggcgt catcgccctc
     1141 tgcgacttcg gcggcagcgg caccagcatc acgctggtcg acgccggcgc cggccaaccg
     1201 gtcggcgaga cggtccgcta cgccgacttg tccggcgacc tgatcgacac ggccgtgctg
     1261 acgcaggtcc tcgagggcct gcccgacgcc ggatccgccg acgtgacggg cacgtccgcg
     1321 atcggtccgc tgagcaggct gcgcacccag tgccgggccg ccaaggagcg gttgtccacc
     1381 acggcggcca cctcgctggt cgccgaactg cccggacagc gcggcgagat ccggctcacc
     1441 cgcaacgagc tcgacgactc gatccggcaa ccgctgaccg atttcgccgg ggccctgcag
     1501 gagacccttg atcgcaacgg cgtacgcgcc ctggccgccg tcgcctccgc gggcggcggc
     1561 gcgcgcatcc ccctcatcac gacgacgctg tcggagcact tcggtgtccc ggtcgtcacc
     1621 accgctcagc cagaattgac ggccgcgatc ggcggcggct tgaacgccgc ccgcggcacg
     1681 gccgaggagg gtgcgacggc cttggccgaa gcgctgagtc cgcccaccgc agcggccgcc
     1741 gcggtggcgg ccacccagat ggcgtcggag gtccacccgg atgacatggg cgccggcccg
     1801 tcctcagggc tggcctggtc cgaggccgac gacgttcccg atctggtcgc gtcgaccgat
     1861 cagtacgact acgcgggcgc ggcacccacc gacgggatgg ccggcccgcg tccgccgatg
     1921 cagttcgaac cggaaaccga ccagtacgag gccgagcggc ggcgaagtcc gttcgccctg
     1981 ctgatcgctg gcctggtcgt ggtgttgctc gccatcgcgt tggcggtgtg gttcgtgctg
     2041 cggaacaacg agagcacgcc ggcgccgtcg tcgacgacca cgaccgccac gaccacggcg
     2101 gccgttccac cgccggcatc cgaggctccg ccgcctgcga gcgaggtgcc gccgtcggag
     2161 gagccgccac cggtgaccca gaccatcacc cccggaccgg agacggtcac gcaaacccag
     2221 gagccgccgc cgcctccgcc caccagcgag gcgcctccgc cgccgacatc agaggctccg
     2281 ccgccgcctg cgtcgccaga gccgccgccg tcgcagcccc cgtcgagtcc gccgcgacta
     2341 atcccgaact accccaccat cccgggccta cccttcgttc cggcacccgt aatacctccg
     2401 gctccctagg ctctcgctca tgactcagac ggacggcctg ctgttcaacc cgaacaccta
     2461 tgacccgcag cagttcgacg ccgagactcg acggctactg cgcgcgacca tcgacttctt
     2521 cgaggggcag ggcaagaaac gcctgctcga cgacgacctc aaggtggtgt ggcctgccga
     2581 cttcgtcgac ttcgtcaagc gcgagaggtt gttcgcgacg ttcctgacgc cgtccgaatt
     2641 cgcgggagcg gacgagggga gcaccacaag cactggagtg tcgaacaagc gctgggacgc
     2701 cgcccgcaac gcggcactgt cggagatctt cggcttctac gggctgacct actggtacac
     2761 cgagcaggtc accatcctcg gcctcggccc gatctggcag agcgagaaca aggacgccaa
     2821 gctcaaggcg gccgacgacc tcgatgccgg cgaggtgatg gccttcgggt tgtccgagcg
     2881 cgaacacggc gccgacgtgt acaacaccga catggtgctc acgcccgcga gcgaggagga
     2941 ccgcgccaac ggaatcctct atcgcgcgac gggggagaag tactacatcg gcaacggcaa
     3001 tgtcgccagc atggtgtcgg tcttcggccg tcgcgacgat gccgaagggc aggacgccta
     3061 cgtctttttc gccgccgaca gccgtcacga gaactatcac cttgtcgaca acgtcgtgca
     3121 catgcagatc tacgtcagca cattccggct ggagaactat ccggtgcgcg cggaagacat
     3181 cctgcatacc ggcgtggaag cctttgcggc cgcactcaat acggtgaaca tcgggaagtt
     3241 caacctgtgt acgtgctcca tcgggatggt cgaacacgcc ttctacgagg cgatcacgca
     3301 cgcgcagaac cggatcctgt acggcaaccc ggtgaccgac ttcgcgcatg tgcgggccaa
     3361 cttcgtcgac tcctatgccc gcatcgtggc gatgaaaatg ttcagccagc gtgcggtcga
     3421 ctacctgcgc agcgcgagcc tcgacgacag gcgctacctg ctgttcaatc ccgtcaccaa
     3481 agccaaggtc acgatggagg gcgagaacat catgcgggcg ctgcacgatg tggtcgccgc
     3541 caagggatat gagaagaaca c
//
LOCUS       DASYLX010000177         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1653918,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000177 DASYLX010000000
VERSION     DASYLX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653918"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGP27_04310"
     CDS             complement(<1..338)
                     /locus_tag="VGP27_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEV7850575.1"
                     /translation="MRAVLIVNPNATSTTRAGRDLLAHALESRVKLTIAHTDSRGHAV
                     DIARDAAREGIDLLIVHGGDGTVNEVVNGVLGECGPTPHPDAPALGVVPGGSANVFAR
                     ALGIDPDPIEA"
     gene            378..770
                     /locus_tag="VGP27_04315"
     CDS             378..770
                     /locus_tag="VGP27_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850576.1"
                     /translation="MTVPSPTTVRQAAVLVALEGAAGVLAAVIYVVSGLDSDDQPGMN
                     KFGTAAWFAILGGAVLGAGWALWRGRRWGRGVAVFAQLLLLPVSWYIAVGSRQWVYGI
                     PVGVVALITLVLLFSPSAVRWLGSADDH"
     gene            complement(764..1258)
                     /locus_tag="VGP27_04320"
     CDS             complement(764..1258)
                     /locus_tag="VGP27_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7850577.1"
                     /translation="MIIRRAEPGDEVELTAMIHELAEFERAAPDCTVTETQLRTALFG
                     DDPTVHGHIAEEDGRAAGGALWFRNFSTWDGVGGIYLEDLFVRPQFRRRGLAKAMLAT
                     LARECVENGYTRLSWAVLDWNVNAIALYDAVGGHQQDEWITYRVSGPELSALAADSSE
                     PPPQ"
     gene            complement(1255..2352)
                     /locus_tag="VGP27_04325"
     CDS             complement(1255..2352)
                     /locus_tag="VGP27_04325"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEV7850578.1"
                     /translation="MTREPTFVLATGGSAVVADGVHTAYRSIAEARTSLTSHSTPIIV
                     GALPFDISRPAALIRPQDVAFLDALPNWPLRPLPGVRVAETLPEPGEHRARIAAALQR
                     LRDPASGLHKVVLARALRLVADGPLDARTVVERLVTADPGTNAYLVDLSAAGADYSGT
                     VLVGASPELLVARQGERVFCAPFAGSAPRLCDPDADRASGEALAASAKNRHEHELVVD
                     AMRKALDPLCVDLQIPLTPQLSRTAAVWHLSTPITGRLRERSTTALDLAISLHPTPAV
                     GGVPANAAAGLIGELEGDRGFYAGAVGWCDQRGDGRWVVSIRGAQLSADRRSAVAHAG
                     GGIVAESDPDDEVEETTTKFTTILSALEAQP"
     gene            complement(2349..2960)
                     /locus_tag="VGP27_04330"
     CDS             complement(2349..2960)
                     /locus_tag="VGP27_04330"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEV7850579.1"
                     /translation="MSPQQHRLLLLRHGETEWSRSGKHTSRTDLDLTETGRRQAKADG
                     ETIAQLNLENPYIVSSPRKRAIVTAELAGLSVDEVNPLISEWDYGDYEGTTTEEIRKA
                     VPNWLVWTHGCPGGETTAEVCERADRAIALALEHMESRDVVFVGHGHFSRAVITRWIE
                     LPVYEGIRFAMAAGSIAVCGFEHGVRQVNALGLTGYPHPAVFP"
     gene            3019..>3242
                     /locus_tag="VGP27_04335"
     CDS             3019..>3242
                     /locus_tag="VGP27_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7850580.1"
                     /translation="MTRVLAVANQKGGVAKTTTVASLGAAMAEKGRRVLLVDLDPQGC
                     LTFSLGQDPDKLAVSIHEVLLGDVEPSAALV"
BASE COUNT          462 a         1084 c         1164 g          532 t
ORIGIN      
        1 gcctcgatcg ggtcggggtc gatgccgagc gcgcgggcga acacgttggc cgacccgccg
       61 ggcaccacgc cgagggcggg ggcgtcgggg tgcggggtcg ggccacattc gccgagtacg
      121 ccgttgacga cctcgttcac cgttccgtcg ccgccgtgca cgatcagcag gtcgatgccc
      181 tcacgcgcag cgtctcgggc gatgtcgacg gcgtggccgc gactgtcggt gtgggcgatc
      241 gtcaacttca cccggctctc cagcgcatga gccagtaggt cgcgaccggc acgggtggtc
      301 gacgtcgcgt tcgggttgac gatcagcacg gcgcgcacga ctcaagagcc taactgcgaa
      361 gctctacgct cgacgtcgtg acggttccat cgccgacgac tgttcggcag gccgccgtgc
      421 tggtggcgct ggagggcgca gcgggcgtgc tcgcggcggt catctacgtc gtgagcggac
      481 tcgacagcga cgaccagccc ggtatgaaca agttcggcac cgcggcgtgg ttcgcgattc
      541 tcggcggggc agtgctcggg gcgggctggg cgttgtggag gggccgccgg tggggccgag
      601 gggtcgcggt gtttgcgcag cttctgctgc tgccggtgtc gtggtacatc gccgtcggct
      661 cccgtcaatg ggtctacggc ataccggtcg gcgtcgttgc gctgatcacg ctggtgttgc
      721 tgttcagccc gtcggccgtg cggtggctag ggtccgccga cgatcactga ggaggcggct
      781 cggacgaatc cgcggccagc gccgacaact cagggccaga cacccgatag gtgatccatt
      841 cgtcctgctg gtggccgccg acggcgtcgt agagcgcgat cgcgttgacg ttccagtcca
      901 gcaccgccca ggacagccgc gtgtagccgt tctcgacgca ttcgcgcgcc agcgtggcca
      961 gcatcgcctt ggccagccca cgacggcgaa actgcggccg cacgaagagg tcctccagat
     1021 agatgccgcc gacgccgtcc cacgtcgaga agttgcggaa ccacagcgcg ccccccgccg
     1081 ccctgccgtc ctcctcggcg atatggccgt gaacggtcgg gtcgtctccg aaaagggcgg
     1141 tgcgcagctg tgtttcggtc accgtgcagt cgggggccgc ccgctcgaat tcggcgagct
     1201 cgtgaatcat cgccgtcagt tcgacctcgt cgccgggctc ggcgcgccgg atgatcatgg
     1261 ttgcgcctcc agagccgaca gaatcgtggt gaatttcgtt gtggtttcct cgacttcgtc
     1321 gtcggggtcg gattcggcga cgatcccgcc gccggcgtgc gcgacggcgg aacggcggtc
     1381 agccgacagt tgcgcgccgc ggatggacac cacccagcgc ccgtcgccgc gctgatcgca
     1441 ccagccgacc gcgcccgcat agaaaccgcg gtcaccttcg agctcgccga tcagccctgc
     1501 ggccgcattc gccggaaccc cgccgaccgc aggtgtcgga tgcagcgaaa tcgcgagatc
     1561 caatgcagta gttgatcttt cgcgtaatcg gccggtgatc ggcgtggaca ggtgccagac
     1621 cgctgcggtg cggctcagct gcggagtcag cgggatttgc aggtcgacgc acagcgggtc
     1681 gagtgctttg cgcatcgcgt cgacgaccag ttcgtgctcg tgacggttct tggccgacgc
     1741 ggccagcgcc tctccgctgg cccggtcggc gtctggatcg cataaccgcg gcgccgaacc
     1801 cgcgaacggc gcgcagaaca cgcgctcgcc ctggcgggcc accagcagct cagggctggc
     1861 acccaccagg acggtcccgg aatagtcggc tcccgccgcc gacaggtcga cgaggtaggc
     1921 gttggtgccg gggtcggcgg tgacgagacg ctcgacgacg gtgcgcgcgt cgagcgggcc
     1981 gtcggccacc aaccgcagcg cccgcgccag caccactttg tgcagcccgc tcgcggggtc
     2041 tcgcagccgc tgcagcgccg ccgcgatccg ggcgcggtgc tcgccgggct ccggcagcgt
     2101 ctccgcgacc cggacaccgg gcagcgggcg caacggccag ttcggcagcg cgtcgaggaa
     2161 agccacgtcc tgcggccgga tcagcgcagc cggcctcgag atgtcaaagg gcaaggcgcc
     2221 gacgatgatg ggggtgctgt gcgatgtcaa cgaggtgcgc gcctcggcga tgctccggta
     2281 cgcggtgtgc acaccgtcgg cgacgacggc gctgccgccg gtggccagga cgaacgtcgg
     2341 ttcgcgcgtc acgggaacac ggctgggtgt ggatatccgg tcagcccgag ggcgttgact
     2401 tgacgcacgc cgtgctcgaa gccgcacacg gcgatcgacc cagcggccat cgcgaagcgg
     2461 attccctcgt acaccggcag ctcgatccag cgggtgatga ccgcgcgcga gaaatgtccg
     2521 tgtccgacga agaccacatc ccgggactcc atgtgctcca acgccagcgc gatcgcccgg
     2581 tcggcgcgct cgcagacctc ggcggtggtc tcgccgccgg ggcagccgtg ggtccacacc
     2641 agccagttcg gcacggcctt gcggatctcc tcggtggtcg tgccctcgta gtcgccgtag
     2701 tcccattcgc tgatcagcgg gttcacctcg tcgaccgaca ggccggccaa ttcggcggtg
     2761 acgatcgcgc gtttgcgggg gctgctgacg atgtaggggt tctccaggtt cagctgggcg
     2821 atggtttccc cgtccgcttt cgcctgtcgc cggccggtct cggtgaggtc gagatcggtg
     2881 cggctggtgt gcttgccgct ccgggaccac tcggtctcgc catgtcgcag cagaagcagc
     2941 cgatgctgct ggggactcac cccgccgatt ctgccccacg ggaggtgctg atgtccccgg
     3001 tcgtgcttgg atggactcgt gacgcgggta cttgcggtcg ccaatcaaaa gggtggggta
     3061 gccaaaacga cgacggtggc gtcgttgggt gccgcgatgg cggagaaggg gcggcgggtg
     3121 ctgctcgtcg acctggatcc ccaggggtgt ctgacgttct cgctcggcca ggaccccgac
     3181 aagctcgcgg tctcgatcca cgaggtgttg ctcggcgacg tggagccgtc ggccgccctg
     3241 gt
//
LOCUS       DASYLX010000178         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3079789,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000178 DASYLX010000000
VERSION     DASYLX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079789"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VGP27_04340"
     CDS             <1..983
                     /locus_tag="VGP27_04340"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7850581.1"
                     /translation="PVKGVPPRRVVVRTTDFDDARRAMEHTFLPMAMWPMEPLAAGLN
                     MLLDSARVDEMTTSTVTFGRCIGLRSVESRTYHVAVPVTGVAESQAGLREPIVTTQEQ
                     AAIFSVDESFAIHWRGDCTQMCLDFPGAALNSRLEQHLDVPIGKPLAFEAAMDLTTPK
                     GRGWLRLLRLANREARRPHGIMENPLTAKSIEAALMDGLLLAQRHNYSDALLRDASAA
                     PPRAVRDAVELLETSPERAWSVPLLAKEVHVSVRSLQEGFRRSLDTTPTRYLRAVRLR
                     KVREDLLSAAAEDTTVGAIVYGWGIVNQGRFANSYQRKFGETPSDTLRGR"
     gene            1070..1459
                     /locus_tag="VGP27_04345"
     CDS             1070..1459
                     /locus_tag="VGP27_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7850582.1"
                     /translation="MTRPDDRDDSADGNNPPLTVQLDLIRPAVPLLQASGVLIPATAD
                     TLLAHLDRELGNGARAIVLDLSGLDVLGVDAVPALVEVAQRAGEVDIGLYVVASDGIV
                     MQALRTAGDLDLFEVYGDVADAVAAIP"
     gene            complement(1506..>1692)
                     /locus_tag="VGP27_04350"
     CDS             complement(1506..>1692)
                     /locus_tag="VGP27_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850583.1"
                     /translation="DNTANAEVVSGWIEKWSPLATEALETFAGVTAEAPVPSDPSEVA
                     ARITDEVSRELSAALER"
BASE COUNT          278 a          565 c          548 g          301 t
ORIGIN      
        1 cgcctgtcaa gggtgtgccg ccacgtcgag ttgtggtgcg cacgacagac ttcgacgacg
       61 cccgccgggc catggagcat acgttcctgc cgatggcgat gtggccgatg gaaccgctgg
      121 ccgccggctt gaatatgctg ctggactccg cccgggtcga cgaaatgacc acaagcacag
      181 tcacgttcgg ccgctgcatc ggacttcgct ctgtcgagtc acgcacgtac catgttgccg
      241 tccccgtgac cggggtcgcc gaatcacaag cgggattgcg cgaaccgatt gtcacaaccc
      301 aggagcaggc ggcgatcttc tcagtcgatg agtccttcgc gattcactgg cggggtgact
      361 gtacgcagat gtgcctggac ttcccggggg cagcgctgaa ttccagactc gagcagcatc
      421 tcgacgtgcc gattggcaag cccctcgcct tcgaggccgc gatggatctg accacgccga
      481 agggtcgcgg gtggttacgg ctgctgcgtt tggccaaccg cgaggcacgt cgtccccatg
      541 ggattatgga gaatccgctc accgcgaaaa gcatcgaggc cgccttgatg gacggacttc
      601 tgctggccca gcggcacaac tacagcgacg cgctcctgcg cgacgccagc gccgccccac
      661 cacgcgctgt gcgcgacgcc gtcgaactcc tcgagacctc accggagcga gcctggtcgg
      721 taccgttgct ggccaaagag gttcacgtca gtgtccgttc gctgcaggag ggcttccgac
      781 gttctctcga cacgacgccg acgcgctatc tccgcgcggt gcgcctgcgc aaggtacggg
      841 aggacctgtt gtcggcggcg gctgaggaca cgacggtcgg cgccatcgtc tacgggtggg
      901 gcatcgtgaa tcagggacgc ttcgccaact cgtatcaacg taagttcggc gaaacaccgt
      961 cggacaccct gcgtgggcgc tgacccttct gatcactatt gcgccaatcg aacaatgctc
     1021 tgcgcaatcc gtacggtttg tccgccgtcg catgcatatg actggagttg tgaccaggcc
     1081 agacgaccgc gacgattcgg ctgacggaaa caacccgccg ttgaccgtcc agctggacct
     1141 catccgaccg gcggtgccgc tcctgcaagc gtccggcgtg ctgatcccgg cgacggctga
     1201 cacgctgctc gctcatctcg accgtgagct cggcaacggg gcgcgggcaa tcgtgctcga
     1261 cttgtccggg ctggatgtgc tgggtgtcga cgccgttccc gccctcgtcg aggtcgcgca
     1321 gcgggcggga gaggtcgaca tcggcctgta cgtggtcgca agcgacggca tcgtgatgca
     1381 ggcgctacgc acagccggcg acttggacct cttcgaggtc tacggcgacg tcgcggacgc
     1441 cgtggccgca attccctgac gtcagtccgg tgtgacggcg gtcgctcggt ttcgcgcgag
     1501 acccgtcagc gctccagcgc cgcactcaac tcacgcgaca cctcgtcggt aatgcgggcg
     1561 gccacctcgc tggggtcgga cggcaccggc gcctccgcgg tgacgccggc gaaggtttcc
     1621 agcgcctcgg tcgccagcgg actccacttc tcgatccagc ccgacaccac ctcggcattg
     1681 gcggtgttgt cg
//
LOCUS       DASYLX010000179         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1763299,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000179 DASYLX010000000
VERSION     DASYLX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763299"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..965)
                     /locus_tag="VGP27_04355"
     CDS             complement(372..965)
                     /locus_tag="VGP27_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850584.1"
                     /translation="MPRAKQRTPELRDRVLDVAITTLGEEGIARFTTRRVAERAGTSV
                     PAVYELFDDKAGLVRAMFFEGFRLLGAELARIPETDDALGDLERLVPVFRLFCRAYPK
                     LAQVMFSRPFADFEPGTDELAAGAAVREIFVGRIQRCVDKGLLSGDPTDIAHVLLALA
                     QGLAVQEAGRWLGTSAEIERRWKVGVTSVLDGFGWAN"
     gene            complement(975..1676)
                     /locus_tag="VGP27_04360"
     CDS             complement(975..1676)
                     /locus_tag="VGP27_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7850585.1"
                     /translation="MTNPVNYELDESVATIALDDGKVNVLGPTMQAAINEALDRAEKD
                     DAKAVVLAGNQRVFSGGFDLSVFQSGDAQAALAMLAGGFELSIRCLTFPKPVIMAATG
                     PAIAMGSFLLLSGDHRVGAPKSRCQAIEVAIGMTIPISAIEIMRMRLTPAAFQRGTSM
                     ASTFVGDEAIAAGWLDEIVDADKVLARAQEVAVEAAATLHAGAHLATKLKARESALTA
                     IRAGIDGLPAEFTAS"
     gene            complement(1760..2758)
                     /locus_tag="VGP27_04365"
     CDS             complement(1760..2758)
                     /locus_tag="VGP27_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850586.1"
                     /translation="MGITEWFDDRAGDIAGLSPGAWTAMAAWLAVCVTIAALVYAWRQ
                     FQSAKLESEDLSQPNVAMFMEPSASDWHLVELVVKNFGQRPAYGIQLEFASPPTVPRY
                     ESADADNYVDIVPLKLPAEIPYLAPSQEWRTVWDSALDRKALGELIGSRFAGAVTFYD
                     QPRSSRQGKTKTKPHQYRTPAVLDWATLHPVERLELLTNHDLARQEKQKLELLRGLLT
                     YYHYATTESRHEVLQAEINKINAAANEQRDRYRSHIAVDQDAPQPKTPAAPVAVEELP
                     SQPQPRYEVAPPQPRYDGLPPEYDDRPPPYDDEPPYDDDDAQTRVINGRHRRGLGG"
     gene            complement(2896..3954)
                     /locus_tag="VGP27_04370"
     CDS             complement(2896..3954)
                     /locus_tag="VGP27_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEV7850587.1"
                     /translation="MKNIVLCFDSTNNRPGSRDATNAEILFRLLDAGADDRQLTWYDA
                     GEAALSSPRLFNPAARRWRDNVAAAARDSVVEAYFFLIENWEPGDRIFLLGAGRGAYC
                     ARTLARMLGTVGMMADREDHFVQYALAAYALPAQSRTTSEWRQIHQLACALEERSDVA
                     VPVHFLGLWDTIKVPGRPSLRDDDWLGNVVSGRHAVAIDGGSGPFGECRTGRDDDTVE
                     EVWFRGAHCDVVGGPNAYWPLADITLDWMLDGVVKAGALVRTGEIPCAPAPSERDALA
                     EGASSLRFRKMPEHALVHASVDMYVRAHSDYWRRLPRHILWTDLDWAARAERLPVPDT
                     TPLAKAQPDPEELAAVAS"
BASE COUNT          731 a         1426 c         1371 g          790 t
ORIGIN      
        1 tgaggctgcg acgggcgttg atgtacatgg tttcggtaaa tgtgctcacg acaattcctt
       61 tggctccggc ggttcgacgc gcggtggtgc gcgtagtgaa ccggcgtgca gacgctgact
      121 gagccgctga ggtgcgacca tcgtccattc ttttcgaggt ggacgactac tgcgggagga
      181 ctccgcccct tcttgttaac ctcagatgaa tctctgcact ccggcaggag cgcggtgggg
      241 cagtgttaag gacttcggac gttctgactg tacagaccta cgcgtcaaag ggcagtgcac
      301 cgcgccgaga cgagtttcag cgcgcaaagt cgttccgtca acagtgatct cgttcgcgta
      361 ccaaattgac gtcaattcgc ccagccgaac ccgtcgagga ctgacgtcac cccgaccttc
      421 catctgcgct cgatctcggc cgacgtgccc agccagcgac ccgcctcctg caccgccaaa
      481 ccctgtgcga gagccagcaa tacgtgcgcg atatccgtcg ggtcgccgct tagcaggccc
      541 ttgtcgacgc accgctggat gcggccgacg aagatctcgc ggacggccgc acctgcagcc
      601 aattcgtcgg tgcccggctc gaagtctgcg aacggccgcg agaacatcac ctgcgccagt
      661 ttcggatacg cgcgacagaa cagccggaac accggcacca gccgctcgag atccccgagc
      721 gcgtcgtctg tctcgggaat tctggccagc tcggcgccca gcagccggaa accttcgaag
      781 aacatcgccc gcaccaagcc ggccttgtcg tcgaacagct cgtatactgc gggcaccgac
      841 gtgccggccc gctcggccac ccgtcgtgtg gtgaaccgcg caatgccctc ctcgccgagc
      901 gtggtgatcg ccacgtcgag cacccgatcc ctgagctcgg gcgtgcgttg cttcgccctg
      961 ggcatcgcag tgacctagct ggcggtgaat tctgcaggca ggccgtcgat tccggcgcga
     1021 atcgccgtca gcgcggattc gcgtgccttc agcttggtcg ccagatgcgc gcctgcatgc
     1081 agcgtcgccg cggcctcgac cgccacctcc tgcgcgcgcg ccagcacctt gtccgcgtcg
     1141 acgatctcgt cgagccagcc ggcggcgatc gcctcgtcgc cgacgaatgt cgacgccatc
     1201 gacgtgccgc gctgaaacgc agcaggcgtc agccgcatcc gcatgatttc gatcgcggag
     1261 atcggaatgg tcattccgat ggccacctcg atggcctgac agcgcgactt cggggcgccg
     1321 acgcggtgat cgccgctcag cagcagaaac gagcccatcg cgatggccgg gcccgtcgcg
     1381 gccatgatga ccggcttcgg gaacgtcagg catcggatgg acaattcgaa gccgcccgcc
     1441 agcatcgcca gggcagcctg cgcgtcgccg gactgaaaga ccgaaaggtc gaatccgccg
     1501 ctgaacacgc gctgattgcc tgccagcacg acggccttgg catcgtcctt ttcggcgcgg
     1561 tccagcgcct cgttgatggc ggcctgcatc gtcggcccga gcacgttgac cttgccgtcg
     1621 tcgagcgcga tcgtcgcgac ggactcgtcg agctcgtagt tcaccgggtt ggtcatcggg
     1681 gctcccttga cgtgggcatt cgtttcagat cgatgttacg aaacgatgtt ccgaaagcca
     1741 agtgccgaat gtcggcgagt catccgccca ggccgcggcg atgcctgccg ttgatcaccc
     1801 gggtctgggc atcgtcgtcg tcatagggcg gctcatcgtc gtacggcggt ggccgatcgt
     1861 cgtactccgg aggcaggccg tcataccgtg gctgcggcgg cgctacctca taccgtggct
     1921 gcggctgcga cggaagttcc tcgaccgcca ccggagccgc aggcgtcttc ggctgcggag
     1981 cgtcctggtc gacggcgatg tggctgcggt agcggtcacg ctgttcgttg gcggccgcgt
     2041 tgatcttgtt gatctcggcc tggagcactt cgtggcggct ctcggtcgtc gcgtagtggt
     2101 agtaggtcag cagaccgcgc agcagttcga gcttctgctt ctcctgacgc gccaggtcgt
     2161 ggttggtcaa cagctccagc cgctcgacgg ggtgcagcgt cgcccagtcc agcacggccg
     2221 gcgtgcggta ctgatgcggt tttgttttcg tcttaccctg tcgggaggac ctcggctggt
     2281 cgtagaacgt gaccgcaccg gcgaaccgtg acccgatcaa ttcgcccagc gccttgcgat
     2341 ccagcgccga atcccacacc gtgcgccatt cctgcgacgg agcgagatag ggaatctccg
     2401 cgggcagctt cagcggaacg atgtcgacat aattgtcggc atcggcgctc tcatagcgag
     2461 gcaccgtcgg cggcgacgcg aattccaatt ggattccata cgccggtctc tggccgaagt
     2521 tcttcaccac cagctcgacg agatgccaat ccgacgcgct cggctccatg aacatcgcca
     2581 cattcggctg cgagagatct tcgctctcga gcttggccga ctggaactgc cgccacgcat
     2641 ataccagcgc ggcaatggtg acgcagacgg caagccaagc cgccatcgcc gtccacgcgc
     2701 cgggggataa gcccgcaata tcgccggcgc ggtcgtcgaa ccattccgta attcccacgc
     2761 gtcgtctata ccatgatcag ctgaggagaa cagcattcga gaagcgcgca ttacaacgat
     2821 ttgctgcagc ggcaattctg gtgagttaat tgaggcagaa gtgcctcacg gtgtcgttcg
     2881 cacagattgc cgacgttacg atgcgaccgc ggcgagttct tcgggatcgg gctgggcttt
     2941 cgccagcggc gtggtgtcgg gaacgggcag tcgctcggcg cgtgcggccc aatccagatc
     3001 ggtccacagg atgtgcctcg gcagccgccg ccagtagtcg gagtgcgccc gcacatacat
     3061 gtcgacgctg gcgtgcacga gcgcatgctc gggcatcttg cggaacctca gcgatgacgc
     3121 gccctcggcg agtgcatccc gctcgctcgg cgcgggagcg cacggaatct ccccggtgcg
     3181 gacgagggcg cccgccttga cgacaccgtc gagcatccag tcgagcgtga tgtcggccag
     3241 cggccagtac gcgttcgggc cgccgaccac gtcgcagtgg gcgccgcgga accagacctc
     3301 ttcgacggtg tcgtcgtccc gtccggtccg gcattcaccg aacggcccag atccgccgtc
     3361 gatggcgacc gcgtgccggc ccgagacgac gttacccaac cagtcgtcgt cgcgaagcga
     3421 cggcctgcca ggcactttaa tggtgtccca caggccgagg aagtgcacag gcaccgcgac
     3481 gtcactgcgc tcctcgagcg cgcacgccag ctggtgaatc tgccgccatt ccgatgttgt
     3541 gcggctctga gcgggcaagg cgtatgccgc aagcgcgtac tgcacgaagt ggtcctcgcg
     3601 gtcggccatc atccccaccg tgccgagcat tctggccagg gtccgtgcgc agtaggcgcc
     3661 acgtccggcc ccgagcagga agatccggtc gcccggttcc cagttctcga taaggaagaa
     3721 gtacgcctcg acgacggagt cgcgggcggc cgccgcgaca ttgtcgcgcc agcggcgcgc
     3781 ggcggggttg aagagtcgcg gtgagctgag cgctgcctca cccgcgtcgt accaggtcag
     3841 ctgccggtca tcggcgccgg cgtcgagcag ccggaacaag atttcggcat tggtcgcgtc
     3901 acgagaaccc ggacgattgt tggtgctgtc gaagcacaac acgatgttct tcatagcaaa
     3961 ctccctgacc acacgtccca cgggctcgag acgttgacag tgcgagtttg ccaggcgtgt
     4021 cgggtcgtgt cttgcgtatt gcactactcg atgtctcggc tcttcgattt ccttcgcgga
     4081 tgcgcgcgat cagcacggat tcagttgtcg acggcatggc gccagcgtca tatcgctgca
     4141 acctcggagt catctgccca ggtcaccgtt gtgggccaac gctgcggtgt tcgccacacc
     4201 gtttcgcgcg ggctgccccg atccgttgct gtactcattc aagactggcc gaaagcgcca
     4261 gtttgttctg ctacatagca accgcggcga cgatcgtcgg ctaagcgacg ggcagcac
//
LOCUS       DASYLX010000180         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_776446,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000180 DASYLX010000000
VERSION     DASYLX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776446"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGP27_04375"
     CDS             <1..804
                     /locus_tag="VGP27_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7850588.1"
                     /translation="WADTGNQGLLIVANRRSSRPPDAERPLGYGREAYVWSLLAAVGL
                     FVVGGTLSVWRGVTELLHGEAAAENYLVAYVVLGVAFLLESSSLFQAFRQLRGEAAQF
                     DRDLLDHVLETSDPTTRAVFAEDSAALIGIALAVAGIGLHQITGDAVWDAIGSILIGI
                     LLGVVAVILIDRNRRYLVGEPASPQLREAAVARIEQLPEVASVRFIRLVFVGPKQLFL
                     VASVDLVGDAVETQIAKTLRQLENQLQDEPYIVDAVLTIAEPDDLDRQL"
     gene            882..>3999
                     /locus_tag="VGP27_04380"
     CDS             882..>3999
                     /locus_tag="VGP27_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850589.1"
                     /translation="MAAVVACGSCATVLRENAKFCDECSAPTAVSAETAKYKQVTVLF
                     ADLVHSMDIAAAVDLERLREIMTELLNRSAAVVKRYGGTVEYNGDGVMALFGAPVALE
                     DHAFRACLTALAIQDEADRLAAEVARRDGVALQLRVGLNSGRVIAGEIGTGSLGYAAI
                     GETVGFAQRMESVAPPGVVMVSESTARLVEHTVMLAEPEWVRVKGIDAPVCARRLVAT
                     RPRDGLVRRAEVGMVGRRWEMAALEAIVDRAIGGRGGVVNVGGPPGIGKSRVAREAAA
                     LAAGRGVEVFWTFCESHARDVPFHAVTRLLRAGSGVADLDGEAARAQLRASAPSDADP
                     QDLLLLDDLLGIADGDVPLPPIEPDARRRRLTALINATSLARTEPALFIIEDAHWVDA
                     VSESMLADFLTVIPRTPSMVLITARPEYEGTLTRVRGAQTIALAALANSDIATLLGEL
                     LGRDPSVGELAAIIADRAAGNPFFAEEMVRELVQRGVLAGEPGGYLCHVDVAEVSVPA
                     TVQAAIEARIDRLSTPARRTLDAASVIGARFGADLLAALVIDPMFGELLNAELIDQVR
                     FTRPAEYAFRHPLIRTAAYESQLKSDRAAWHRRVAAAIQERDPGSADENAALIAEHLE
                     SAGELHAAYGWQMRAATWATNRDISAARPSWERARRIADSLPADAADLVAMRIAPLTM
                     LCATAWRVHVSVTDGRFEELRTLCTAAGDKASLAIGMAGLVIDHAYHDRIDEASALAS
                     EHMALTESIGDPDLMVGLCVPVMYAKLESAEYSDLLRWSQRVIELAGGDPSKGDFIFG
                     SPLAYALTSRAIARYCLGRPGWREDLRHGLAMGRSVDPLSYAAAVSLSYFAGIPHGVL
                     RPQDSVVREINDALQIAQRSGDDLAVANVQMALGMALAHRDTAAERGCGRKLLAEVGD
                     VFVSRGHNLAELPIVEVYLARERARRGERDEAIPVMRAAVDQQFREGRLLLWGVAVTS
                     VLVETLLECADRGDVAEAQAAVERLAAAQAADGSALRDIWLLRLRALLARTRGDDAAY
                     RDLVGRYRA"
BASE COUNT          610 a         1238 c         1447 g          704 t
ORIGIN      
        1 tgggccgata ccggcaatca ggggctgctg atcgtggcca atcggcgaag cagcagacca
       61 cccgacgccg agcgtccact gggatacggg cgggaggcct acgtctggtc actgctggca
      121 gcggtcggct tgttcgtcgt cggtggaacg ctgtcggtct ggcgtggtgt cacggagctg
      181 ctgcacggcg aggcggccgc cgagaactat ctcgtcgcct acgtcgtgct cggggtcgcc
      241 ttcctgctcg agtcctcgtc gctgtttcag gcattccgcc aattgcgcgg cgaggccgca
      301 caattcgatc gagatctact cgaccacgta ctggagacat cggacccgac gaccagggcg
      361 gtgttcgccg aggactccgc tgcgctgatc ggtatcgcgc tcgcggtagc cggcattggg
      421 ctgcatcaga tcaccggtga tgccgtatgg gacgccatcg ggtccatcct gatcggcatc
      481 ctgctgggag tcgtcgccgt gatcctcatc gaccgcaacc gccgttatct ggtcggcgag
      541 ccggccagcc cacagttgcg cgaggccgca gtggcccgca tagagcagct gcccgaggtc
      601 gcgtcggtgc gcttcatccg gctggtgttc gtcggaccca agcaactgtt cctcgtcgcc
      661 agcgtggatc tggtgggcga cgcggtcgag acgcaaatag cgaaaacgtt gcggcaattg
      721 gagaatcagc tgcaggacga gccgtacatc gtcgatgccg ttttgacgat cgccgagccc
      781 gacgatctgg accggcagct ctaacggttg ccatttggac atggcctgac gccgatgtca
      841 attgcggcgt cttttccagg ttaaggccct acggtggtcg aatggcggcg gtcgtggcgt
      901 gcgggtcgtg cgccactgtg ctgcgggaga acgccaaatt ctgcgacgag tgcagtgccc
      961 cgacagccgt ttcggcagag acggcgaagt acaagcaggt gacggtgctg tttgccgacc
     1021 tcgtgcactc gatggacatc gcggccgcgg tggacctcga gaggttgcgg gagatcatga
     1081 ccgagttgct caatcggtcg gcggcggtgg tcaagcgata cggcggaacc gtggagtaca
     1141 acggcgacgg ggtgatggcg ctgttcggtg ccccagttgc tctggaagat cacgcttttc
     1201 gggcgtgcct caccgcgctc gccattcagg acgaggcgga ccggttggcg gccgaggtgg
     1261 cgcgccgcga tggcgtggct ctgcagttgc gggtgggcct gaattcgggt cgggtcatcg
     1321 ccggcgagat cggtacgggt tcgctcgggt atgccgcgat cggcgagacg gtcgggttcg
     1381 cgcagcggat ggagtctgtg gcgcccccgg gtgtcgtgat ggtgtcggag tcgacggccc
     1441 gacttgtcga gcacaccgtc atgctggccg agccggaatg ggtgcgcgtc aaggggatcg
     1501 acgcaccggt gtgcgcgcgc cggcttgtgg ctacccgccc cagggatggc ctggtcagac
     1561 gggccgaagt gggcatggtc ggccgacgct gggagatggc ggcgctcgaa gcgattgtcg
     1621 accgtgcgat cggtggccgc ggcggggtcg taaatgtagg cggaccgccg ggcatcggta
     1681 agagccgggt ggcccgcgag gctgccgcac ttgcggcggg ccgcggcgtc gaggtgttct
     1741 ggaccttctg cgagtcgcac gcgcgcgacg tcccgttcca tgcggtgacg cggctgttgc
     1801 gagcgggcag cggagtcgcc gacctcgacg gcgaagcggc ccgcgcgcaa ttgcgcgcca
     1861 gtgcgccgag cgatgccgac ccccaggatc tgctgctgct cgatgatctg ctcggaatcg
     1921 ccgacggcga tgttccgctg cccccgatcg agccggatgc ccggcgtcgg cggctgaccg
     1981 cactcatcaa tgcgacgtca cttgcccgga cggaaccggc gctgttcatc atcgaggatg
     2041 cgcactgggt cgacgcggtc agcgagtcga tgctggccga cttccttacc gttattccgc
     2101 gcacgccgtc gatggtgttg atcacggccc gccccgaata tgagggaacg ctgacgcggg
     2161 tccgcggcgc tcagacgata gcgcttgcag cacttgcaaa ttcggatata gccacgttgt
     2221 tgggcgagct gttgggtcgg gatccttcgg tcggcgagct tgcggcgatc atcgccgacc
     2281 gggccgcagg caacccgttt ttcgccgagg agatggtgcg cgagttggtg cagcgcggcg
     2341 tgctggccgg cgagccgggt ggctacctct gtcacgtcga tgtggcagag gtgagcgtgc
     2401 ccgcaacggt gcaggcggcc atcgaggccc gcatcgaccg gctgagcact ccggccaggc
     2461 ggacgttgga cgcggcttcg gtgatcggag cgcgcttcgg ggcggacctg ctggctgcgc
     2521 tggtaatcga tccgatgttc ggcgaactgc tcaacgcgga gctgatcgat caggtgcgat
     2581 tcacgcgacc cgccgagtac gcgtttcgcc atccgctgat ccgcacggcg gcctacgagt
     2641 cgcagctcaa gtcggaccgc gccgcatggc accggcgcgt ggccgccgcg atccaagagc
     2701 gtgatcccgg ctcggccgac gagaacgcgg cgctcatcgc cgaacatctc gagtcggcgg
     2761 gcgagctgca cgctgcatac ggctggcaga tgcgtgccgc gacgtgggcg acgaatcgcg
     2821 acatctctgc ggcgcgaccg agctgggaac gtgcccgaag gatcgccgac tcactgcccg
     2881 ccgatgctgc agacctggtg gcgatgcgca tcgccccgct caccatgttg tgcgcgaccg
     2941 cctggcgagt ccacgtcagc gtgaccgatg gccgcttcga ggaattgcgg acgttgtgca
     3001 ccgccgccgg ggacaaagcg tcactcgcga tcggcatggc cgggctggtg atcgaccacg
     3061 cctaccacga ccgcatcgac gaggcgtctg cgctggcgtc cgagcacatg gccctcaccg
     3121 agtcgatcgg cgatccggac ctgatggtgg gactgtgcgt gccggtgatg tacgccaaac
     3181 tcgaaagcgc cgagtattca gacctgctgc ggtggtcgca aagggtcatc gagctggccg
     3241 gcggtgaccc ttcgaagggc gacttcatct tcgggtcgcc gttggcgtac gcccttacgt
     3301 cgcgcgccat cgctcgttac tgcctgggtc gtcccggatg gcgagaagat ctgcgccacg
     3361 gcctcgccat gggccgcagc gtcgatccgt tgtcctacgc cgcggccgtc agcctcagct
     3421 acttcgccgg cataccgcat ggcgtgctga ggccgcagga ctccgtggtc cgcgagatca
     3481 acgatgcact gcagatcgcg caacgatccg gcgacgacct cgcggtggcg aacgttcaga
     3541 tggcgctggg catggcgctg gcgcaccgcg acacagccgc cgagcgtggc tgtggacgga
     3601 agctgctggc ggaggtcggc gacgtattcg tgagccgggg gcacaacctc gctgagttgc
     3661 cgatcgtcga ggtctacttg gcgcgtgaaa gggctcgtcg tggcgaacgc gatgaagcca
     3721 taccggtcat gcgcgccgcc gtcgaccagc agttccgtga gggacggctg ctgttatggg
     3781 gcgttgccgt gacgagtgtg ctggtggaga cgctgctcga gtgcgcagac cgcggtgacg
     3841 tagccgaggc gcaagccgcg gtcgaacggt tggcggcggc gcaagccgcc gacggttcgg
     3901 cgctgcgaga catctggttg ttgcggctgc gtgcgctgct ggcgcggacc cgcggcgacg
     3961 atgccgccta ccgggatctg gtcggtcgct atcgcgcga
//
LOCUS       DASYLX010000181         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1763381,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000181 DASYLX010000000
VERSION     DASYLX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763381"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGP27_04385"
     CDS             <1..553
                     /locus_tag="VGP27_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005258616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEV7850590.1"
                     /translation="VQINLPKTALKGPVSLEWKVPQYGSNTIERNRARTYFQAYAAAC
                     ALHFAEKALVEIRAGRTKTWEKFDVPNEGIGCGFTEAVRGVLSHHMVIRDGKIANYHP
                     YPPTPWNASPRDSYDTPGPYEDAVQGQPIFEENDREHFKGIDIMRTVRSFDPCLPCGV
                     HMYLGKGKTLERLHSPTQSVTGE"
     gene            557..1495
                     /locus_tag="VGP27_04390"
     CDS             557..1495
                     /locus_tag="VGP27_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEV7850591.1"
                     /translation="MPHRPDNPGQDAQWRTAGDRIQNLLDASATGGVVAHERAEQLVR
                     EVADLYGAGLERMMGMALTAQPGLADAFSADDLIASLLLVHGLHPHGVERRIEDALDS
                     VRPYLGSHGGDVHLLEVDGDVVRLQFAGSCKSCPSSAVTLELTVEDAVRAAAPEISSI
                     EVVAAETDAKPAAVIPANTLMSRVRTHPPGHMWHPVPDIAELAPGEVAGFAVAGTTVL
                     ACRLGDRLFAYRDRCGSCGESLAGAVLHRRMGSAADEAVLRCPRCRAHFDVVHAGACV
                     DGDNGLGGTDGAHLEPVPLLERDGILSMAVAEAVAS"
     gene            1492..2124
                     /locus_tag="VGP27_04395"
     CDS             1492..2124
                     /locus_tag="VGP27_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEV7850592.1"
                     /translation="MTGAYDVLSRIRAARTAPQPAGERCEMCSEAIADEHQHVVNVEG
                     RQLMCVCRGCYLLFTEEQAKLRFRAVPDRYLAFPDFALDRRHWEVLQIPVGLAFFFRN
                     SALGQTVAFYPGPAGATESELELKAWNEIRAADARVDMLADDTEAVLVRVPDDETAAP
                     QSYLLPIDACYEFVGRLRMLWHGFDGGQQVREFIDEFFALLDDRSKVVTR"
     gene            2191..2835
                     /locus_tag="VGP27_04400"
     CDS             2191..2835
                     /locus_tag="VGP27_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEV7850593.1"
                     /translation="MTDVTFAVIEVQPEPFAVTPVLNARLGIAAVGEDPIHAIALRCQ
                     VRIEPMRRGYSEDEGAGLVDLFGPRERWATTMHHFQWLQTTAMVQGFTGATQVNLPLE
                     CTYDFEVAASKYLHALHDGYVPLLFLFSGTVFSKGQRGFAVQQVPWDREDRLEMPVSV
                     WRDLIRQHYPNTGWLRLNQDTVDALVAYRSARGLLSFDDAVTSLLAASSTEEIR"
     gene            2858..4204
                     /locus_tag="VGP27_04405"
     CDS             2858..4204
                     /locus_tag="VGP27_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850594.1"
                     /translation="MSAANRLRARAVADAVLYEGYLLYPYRANSSKNQARWQFGVLGP
                     AGAADAGIGEDDSLSAQVLVAPRGRPSVSIVVRFLQLQHRGVEKSCGDGRFEPVEELV
                     SDTRSWLRWDEAIEREVSIGPLDLESGLPGVHPIEFDAGTDIEDVDGGRLVRTRRALH
                     AQLEVTADHDDGFIRVTMTVRNTAAHVAHKDDAIAVSLIGTHLVAEVIDGDFVSLLEP
                     PASADNAVARCRHHRCFPVLAGPPGDQALMLVSPIILYDHPEIAEQSEGALYDSTEID
                     EILTLRIMTMTDEEKAAARATDPLAAQIIDRCDSMSPEALLNLHGVLRDPHAVADSAG
                     LIPEVPDGVDWWDPLADNAVAPDVDAVLVNGMRVSRGSRVRLHPSRRADAQDLFFADK
                     IARVTSVHETVDGDQHVGVVIEEDPAADLHEWYGRYLYFAPDEVEPLNERNPTCKQ"
     gene            4316..4810
                     /locus_tag="VGP27_04410"
     CDS             4316..4810
                     /locus_tag="VGP27_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV7850595.1"
                     /translation="MRGAITYQILVAGIGNIFLGDDGFGPEVMRHVPEHVAGPRVRVL
                     DYGIRGMHLAYDLLDGCDALVLVDALPNRGAPGTLHVFEADHETLSAGVGLDAHAMDP
                     AAVFASLAALGGTAPYTVVVGCEVDDVDERMGLSAAVAASVPDAVKAIEEVVGRLVDD
                     AKVG"
     gene            4814..>5022
                     /locus_tag="VGP27_04415"
     CDS             4814..>5022
                     /locus_tag="VGP27_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV7850596.1"
                     /translation="MCLGIPGRVVQMLEGYGGQLALVDVAGENRKVNIGMLPEETFAP
                     GDWVIIHMGFVVEKTDEAGADAAMAG"
BASE COUNT          836 a         1628 c         1727 g          831 t
ORIGIN      
        1 cgtgcagatc aatctgccga aaaccgcgct caagggcccg gtttcgttgg agtggaaggt
       61 gccgcagtac ggcagcaaca cgattgagcg caaccgtgcg cggacgtact tccaggccta
      121 cgccgccgcg tgcgcgctgc atttcgcgga gaaggcactg gtcgagatcc gggcgggccg
      181 aaccaagacc tgggagaagt tcgacgtgcc caacgaaggc atcggctgcg ggttcaccga
      241 agcggtgcgt ggggtgctga gccaccacat ggtcatccgc gacggcaaga tcgcgaacta
      301 ccacccgtat ccgccgacgc cgtggaacgc gagcccccgc gacagctacg acacgccggg
      361 accgtacgag gatgcggttc agggtcagcc gatcttcgag gagaacgacc gcgagcactt
      421 caagggcatc gacatcatgc gcacggtgcg cagcttcgat ccgtgcctgc cctgcggtgt
      481 gcacatgtat ctgggcaagg ggaagaccct ggagaggttg cactccccga cccaatccgt
      541 aaccggggaa tgacatatgc cgcatcgccc agataacccc ggacaggacg cgcagtggcg
      601 cacggcgggc gatcggatac agaacctcct cgacgccagc gcaactggtg gcgtcgtcgc
      661 gcacgaacgc gcggagcagt tggtccgtga ggtagccgac ctctacggcg cgggcctcga
      721 gcggatgatg ggcatggcgc tcaccgcgca gcccggactt gcagacgcct tctccgccga
      781 cgacctgatc gccagcctgc tgctggtgca cgggttgcac ccgcacggcg tcgagcgacg
      841 gatcgaggac gcgctggaca gcgtgcggcc gtatctcggc tcgcacggcg gcgacgtcca
      901 tctcctggag gtcgacggcg acgttgtccg gttgcagttc gcgggcagct gcaagagctg
      961 cccgtcatcg gccgtcaccc tcgagctgac cgtcgaagac gcggtacgcg ctgcagcgcc
     1021 ggagatttcg tcgatcgagg tggtggccgc ggaaactgat gccaagccgg ccgcggtgat
     1081 tccggcgaac accctgatga gccgtgtacg cacgcatccg ccggggcaca tgtggcaccc
     1141 ggtgcccgac atcgccgaac tcgcgccggg agaagtcgcc gggttcgcgg tggcgggcac
     1201 taccgtgctg gcctgccggc tcggtgaccg gctgttcgcg taccgggacc ggtgcggttc
     1261 gtgcggtgag tcgcttgccg gtgcggtgct gcatcgacgc atgggttcgg ccgccgacga
     1321 ggcggtgctg cggtgcccgc gctgccgcgc tcacttcgac gtcgtgcacg cgggtgcctg
     1381 cgtcgacgga gacaacgggc tgggtggaac cgacggagcg catctcgagc cggtaccgct
     1441 gctggagcgc gacggaatcc tttcgatggc ggtcgccgag gcggtggcgt cgtgaccggt
     1501 gcatatgacg tcctttcccg tatccgtgcc gccagaacgg caccgcagcc ggcaggcgaa
     1561 cggtgcgaga tgtgctcgga ggcgatcgcc gacgagcacc agcacgtggt gaacgtcgaa
     1621 ggcagacagt tgatgtgcgt ctgccgaggc tgctatctgc tgttcaccga ggaacaggcg
     1681 aagttgcggt ttcgcgccgt ccccgaccgc tatctcgctt ttccggactt cgcactggac
     1741 cggcggcact gggaggtcct ccagatacca gtggggctgg cgttcttctt ccgcaattcg
     1801 gcgctcggac aaacggtggc gttctatccc ggacctgcgg gcgccaccga gtccgagctc
     1861 gaactcaagg cgtggaacga gattcgcgcc gccgatgccc gggtggacat gctcgccgac
     1921 gacaccgagg cggtgctcgt gcgggtgccg gacgacgaga ccgcggcgcc acagagttat
     1981 ctgctgccca tcgacgcctg ctacgagttc gtcggcaggc tgcggatgct gtggcacggt
     2041 ttcgacggcg gccagcaggt tcgcgagttc atcgacgagt tcttcgctct gctcgacgac
     2101 cgcagcaagg ttgtcacgag atgacttcgc agcgagtagc ggaggcggat cgcgcatgac
     2161 ttcgcagcga gtagcggagg cggatcgcgc atgacagatg tcacgttcgc cgtcatcgag
     2221 gtccagccgg agccgttcgc cgtgaccccg gtgttgaacg cgcggctggg catcgcggcc
     2281 gtcggcgagg atccgatcca cgcgatcgcc ctgcgctgcc aggtgcgcat cgagccgatg
     2341 cggcgcggct attccgagga cgagggcgcc ggtctggtcg acttgttcgg accgcgtgag
     2401 cggtgggcca ccaccatgca ccactttcag tggctgcaga ccaccgcgat ggtgcagggc
     2461 ttcaccggcg ccactcaggt caacctccct ctggaatgca cctatgactt cgaggtggcg
     2521 gcttcgaagt acctgcacgc cctgcacgac gggtacgtgc cgctgctatt cctgttcagc
     2581 ggaacggttt tctcgaaagg gcaacgggga ttcgcggtgc agcaggtgcc gtgggaccgt
     2641 gaggatcgct tggagatgcc cgtctcggtg tggcgcgacc tcatccggca gcactatccg
     2701 aacacgggct ggctgaggct gaaccaggac accgtcgacg cattggtggc gtatcggtcg
     2761 gcgcgcgggc tgctgtcctt cgacgacgcg gtcacctcac tgctcgcggc gagttcgacc
     2821 gaggagatcc gatgaccgcg gactgggatg gtgcctgatg tcggcggcca accgcctccg
     2881 ggcccgcgcc gtcgccgacg cggtgctcta cgagggttat ctgctctacc cctatcgcgc
     2941 aaactcgagc aagaatcagg cgcggtggca gttcggggtg ctcgggcccg ccggtgccgc
     3001 cgacgccggg atcggcgaag acgactcgct gtccgcacag gtcctcgtcg cgccgcgggg
     3061 ccgcccgtcg gtatcgatcg tggtgcgctt tctgcagctg cagcaccggg gcgtcgagaa
     3121 gtcctgcggt gacggccgat tcgagccggt cgaagaactc gtgtccgaca cgcgatcctg
     3181 gctgcgctgg gacgaggcga tcgaacggga ggtctcaatc gggccgctcg acctcgaatc
     3241 gggtctaccg ggagtacatc cgatcgagtt cgacgcgggc accgacatcg aggacgtcga
     3301 cggtggcagg ctggtccgca cccgtcgtgc attgcacgcg caactcgagg tcaccgcaga
     3361 ccacgacgac ggattcatac gcgtcacgat gaccgtgcgc aacaccgcgg cgcacgttgc
     3421 tcataaagac gacgcgatcg ccgtgtcgct catcggcact cacctcgtcg ccgaggtcat
     3481 cgacggcgac ttcgtgtcgc tcctcgagcc gccggcatcg gccgacaacg ccgtcgcccg
     3541 atgccggcac catcgctgct tcccggtgct cgccgggccg ccgggcgatc aggcgttgat
     3601 gctggtatcg ccgatcatcc tctacgacca ccctgagatc gccgagcaga gcgagggcgc
     3661 gctgtacgac tcgacggaga tcgacgagat cctcacgctg cgcatcatga cgatgaccga
     3721 cgaggagaag gcagcggccc gcgcgacgga tccgctcgcc gcgcagatca tcgatcgctg
     3781 cgactcgatg tcgcccgagg cgttgctcaa cctgcacggt gtactgcgcg acccgcacgc
     3841 cgtcgccgac tcggccggcc tgattcccga agtacccgac ggcgtggact ggtgggatcc
     3901 gctggccgac aacgccgtag cgcccgatgt ggacgccgtg ctggtgaacg gcatgcgggt
     3961 gagccgaggt agccgcgtgc gcctgcaccc gtcacgccgg gcggacgcgc aggatctgtt
     4021 cttcgcggac aagatcgccc gcgtcacgtc ggtgcacgaa accgtcgacg gagaccaaca
     4081 tgtcggcgtc gtcatcgagg aagaccccgc cgccgacctg cacgagtggt acggccgcta
     4141 cctgtacttc gccccggatg aggtcgaacc gttgaatgaa aggaatccga catgcaagca
     4201 gtaggtgtca cggccaccgt catagcgggc gtcgttgtgc tcggcgcggt ggtgatgggc
     4261 gtccgatcgc tgccagacct gcagcgatac ctcaagattc ggcgcatgta ggcgattgcg
     4321 aggtgcgatc acgtaccaga ttctggtagc cggtatcggc aacatcttcc tcggcgacga
     4381 cggcttcggg cccgaggtga tgcgccacgt gcccgaacat gtcgcaggcc cgcgtgtgcg
     4441 ggtgcttgac tatggaattc gaggcatgca cctcgcctac gacctgctcg acggatgcga
     4501 cgccctggtg ctcgtcgacg ccctgcccaa ccgcggggct ccgggcacct tgcacgtctt
     4561 cgaggccgac catgaaacgc tctcggcggg tgtcggtctc gacgctcacg ccatggatcc
     4621 cgccgcggtg ttcgccagcc tcgccgctct cggcggaact gcaccgtaca ccgtcgtggt
     4681 cggttgtgag gtcgacgacg tcgacgagcg aatgggcctg tccgcagcgg tcgccgctag
     4741 cgtccccgac gccgtcaagg cgatcgagga ggtcgtcggc cgcctcgtcg acgacgcgaa
     4801 ggtcggctga gccatgtgtc ttggaatccc cggtcgggtc gtgcagatgc tggagggcta
     4861 cggcggtcaa ctcgcgctcg tcgacgtcgc aggcgaaaac cgcaaggtca acatcggcat
     4921 gctgccggag gagacctttg cgccgggtga ctgggtgatc atccacatgg gtttcgtggt
     4981 cgagaaaacc gacgaggcgg gcgccgacgc cgccatggcc gg
//
LOCUS       DASYLX010000182         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2202134,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000182 DASYLX010000000
VERSION     DASYLX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202134"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VGP27_04420"
     CDS             <1..1459
                     /locus_tag="VGP27_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850597.1"
                     /translation="QDFAVALASHVVDGGIAVKVLLIAGLWLAGWGSARLAAAVLPDA
                     GVPGQFLAATLAVWNPYVAERLLQGHWSLLVGYGCLPWVAATVLRMRTDTGALWIEVC
                     ALAFWTALAGLTPTGLMLAATVALVCSLTPGPGRPRWMCAVAGVGAAVLAALPWLTAA
                     AVSESLSSTQADGVPAFAARAEPGLGTLGSLAGLGGIWNSGAVPDSRTTLFAMVSVVV
                     LLGIVAAGLPVAARTPAAVPLLVLAAAAVVVPALMATEPGLAAVESMVRALPGLGVVR
                     DAQKWVALAVPGYTLSAAGAVIMLRRWLPGAATAAVCSLALVATLPDLAWGVGGKVTA
                     VRYPPGWAAVAAMINADPDPVAVLPAGSSMRQFPWAGEAPVLDPLPRWVRADVLTTGD
                     LAVSGRLVTGEGGRAREVERLLTTGADHAALARAGVGWLVVESGDDVTATRVGGDHPV
                     APHRGLMLVTHAVWLAMLVAGGAGALAGLARRRVE"
     gene            1497..2720
                     /locus_tag="VGP27_04425"
     CDS             1497..2720
                     /locus_tag="VGP27_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850598.1"
                     /translation="MVVMSAPSVFEADLPTVEYEDATTPDEAHRNLRKALEQGPIAMG
                     AHGPEILSYELVRATLRDARFQVPRGLGLETQGITDGPLWQRASTSLLAMNGDDHNRL
                     RRLVSKAFTPRSVSRLDDIIVDVITRLTVPLATKGRCEVVADIARPYPVPIISALLGA
                     PRDDWKLFSDWADDFFKLFSWNVAEYENDILTAWGELDDYIDGMVATRRESLTDDLIS
                     ELIRAEDDGDRLTIGELRMLAAGILMAGTDTTRNQVAAALDVFCDYPEQWELLTQRPE
                     LAMKAVEEVMRFNPVIFGAMRTTVEDVELDGVTIPVGTFVVCNTAAANHDADVYVDPD
                     RFDITRADAPPMQTFGAGAHYCLGANLARREMAEALVVMSGRMKNLRRDGLARWKPMV
                     GISGPATLPVAFDAA"
     gene            2846..3760
                     /locus_tag="VGP27_04430"
     CDS             2846..3760
                     /locus_tag="VGP27_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850599.1"
                     /translation="MVEVFLGREALGDGLRRHDLRSWYRPIFRGVYIPKASTPSLHDR
                     TVGAWLTSDRAGVIAGVAASALHGAAWVDDSHPIEILVDERRRQPGLVVRMDRVADDE
                     ITIVDDLPVTTPARTAFDTGRYQQRSVALGRLDALMRAAPFADREVATLMQRYGAVRG
                     IRQLRALLPLVDAGAASLKESWLRLLLIDNGFPIPETQIPVLDNDEPFAFLDMGWRDL
                     LLAVEYDGDQHRTDRPQYVKDVRRIKRIERHGWEIIRVINEDRPHEILAQVYEAWLRR
                     GGVEIDEMAAITRTFPPNRWFGRNGNAA"
     gene            complement(3770..>4849)
                     /locus_tag="VGP27_04435"
     CDS             complement(3770..>4849)
                     /locus_tag="VGP27_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7850600.1"
                     /translation="IGAQLAASGVDVTLRTARYAGAPRNEVVDGVRINRAGGAYSIYI
                     WAGLAMVLARVGLGPLRGVRPDVVIDTQNGLPFLARLAYGRRVVVLVHHCHRELWPVA
                     GRWKGSFGWFVESRLSPWLHRRNQYVTVSLPSARDLTDLGVDAGQIAVVRNGLDEAPP
                     ETLALARAEHPRIALLSRLVPHKQIEDALNAVAELLPRIPGLHLDIVGGGWWRDRLVE
                     HARLLGVADAVTFHGHVDDVSKHEVLQRCWVHVLPSRKEGWGLAVTEAAQHGVPTIGY
                     RSSGGLTDSIVDGVTGILVDDHRDMVVALERLLTDPVLRDQLGGKAQIRSAEFSWRQS
                     ADAMLVVLNSVSKGRRVSGVVSAAD"
BASE COUNT          741 a         1570 c         1745 g          793 t
ORIGIN      
        1 gcaggatttc gcggtggcct tggcgtcgca tgtcgtcgac ggcggcatcg cggtcaaggt
       61 gctgctgatc gccgggctgt ggcttgcggg ctggggcagc gcccggttgg ccgcagcggt
      121 gctgcccgat gcgggtgtgc ccgggcagtt cctcgcggca acgttggcgg tgtggaatcc
      181 ctatgtcgca gaacggcttc tgcaggggca ctggagcctg ctggtcggct acgggtgtct
      241 gccgtgggtg gcggcgacgg tgctgcggat gcgaacggac accggggcgc tgtggatcga
      301 ggtctgcgcg ctggccttct ggacggcgct ggccgggctg accccgacgg ggctgatgct
      361 ggccgcgacg gtggcgctgg tgtgttcgct gaccccaggc cccggccggc ccaggtggat
      421 gtgcgcggtc gccggggtgg gcgcggcggt tctcgcggcg ctgccgtggt tgacggccgc
      481 cgcggtgtcc gaatcgctgt cgtcgacaca ggctgacggt gtgcccgcgt tcgcggctcg
      541 tgccgagccg ggactcggca cgcttggcag cctcgccggc ctcggtggca tctggaacag
      601 tggggctgta ccagactcgc ggacaacgct tttcgcgatg gtgtccgtcg tagtactgct
      661 gggcatcgtc gcggccgggc tgccggtcgc cgcccgcacc cccgccgcgg ttcccctgct
      721 cgtgctggcc gccgccgccg tcgtggtgcc cgccctgatg gcgacggagc cggggctggc
      781 ggccgtggag tcgatggtcc gcgcgctgcc cggcctcggc gtggtgcgcg atgcccagaa
      841 gtgggtggcg ttggcggtgc cgggctacac gctgtcggca gcgggggcgg tgatcatgct
      901 gcggcggtgg ctgcccggcg ccgcgaccgc ggcggtgtgc agtctcgctc tggtcgcgac
      961 gttgccggat ctggcgtggg gagtcggcgg caaggtcacc gcggtgcggt atccgcccgg
     1021 ttgggcggcg gtcgcggcga tgatcaacgc cgacccggac ccggtggcgg tgctgcctgc
     1081 gggcagcagc atgcggcagt tcccgtgggc aggcgaagcg ccggtgctcg acccgcttcc
     1141 gcggtgggtc cgcgccgacg tgctgacgac cggggacctg gcggtctcgg ggcgtctggt
     1201 gaccggtgag ggcggccgcg ccagggaggt cgagcggttg ctgacgacgg gcgccgacca
     1261 cgccgcgctg gcccgggcag gcgtgggctg gctggtcgtc gaatccggcg acgacgtcac
     1321 cgccacccgg gtgggcggcg accaccctgt cgcaccacat cgcggattga tgctggtcac
     1381 gcacgccgtg tggctggcga tgctggtcgc gggcggagcg ggtgcactcg ccggactagc
     1441 caggcggcga gtggagtagc gcactacgct actgaccctc cccggcgggc agaagcatgg
     1501 tcgtcatgag tgcgccgagc gtgttcgaag ccgatctgcc gaccgtcgag tacgaggacg
     1561 cgacgacgcc cgacgaagct caccgcaacc tacgcaaggc gctcgagcaa ggcccgatcg
     1621 cgatgggtgc ccacggcccc gagatcctca gttacgagct ggtccgcgcg acgctgcgcg
     1681 acgcccggtt ccaggtgccg cgaggcctcg gcctggagac gcagggcatc accgacggcc
     1741 cgctgtggca gcgagccagc acgagcctgc tggccatgaa cggcgacgac cacaaccgcc
     1801 tgcgccggct cgtgtcgaag gcattcaccc cgcgctcggt ctcgcggctg gacgacatca
     1861 tcgtcgacgt catcacgagg ctgaccgttc cgctcgccac taagggacgc tgcgaggtcg
     1921 tcgccgacat cgcgcggccc tatccggtgc cgatcatctc cgcactgctc ggcgcgcccc
     1981 gcgacgactg gaaactgttc tccgactggg ccgacgactt cttcaagctg ttcagctgga
     2041 acgtcgccga gtacgaaaac gacatcctca cggcctgggg agaactcgac gactacatcg
     2101 acggcatggt cgccacccgg cgagagtcgc tgaccgacga cctgatctcc gagctgattc
     2161 gcgccgagga cgatggcgac cggttgacga tcggcgaact gcggatgctg gcggcgggca
     2221 tcttgatggc gggcacggac accactcgca accaggtggc cgcagcgctc gacgtgttct
     2281 gcgactatcc cgaacagtgg gagctgctga cgcagcgccc ggagctggct atgaaggccg
     2341 tcgaagaggt aatgcgtttc aacccagtga ttttcggtgc aatgcggacg acagtcgagg
     2401 acgtcgaact cgacggtgtg acaattccgg tgggcacatt cgtcgtgtgc aatacggcgg
     2461 cggcgaatca tgacgcggac gtgtacgtcg acccggatcg tttcgacatc acgcgcgcgg
     2521 acgcgccgcc gatgcagacc ttcggggcag gcgcacacta ctgcctcggc gcgaacctcg
     2581 cgcgtcgcga aatggcggag gcccttgtcg tgatgtcggg caggatgaag aacctgcgac
     2641 gggacggttt ggcgcggtgg aagccgatgg tcggaatcag cggcccggca acgcttccgg
     2701 tcgccttcga cgccgcctag ccccccgcgc ccgttttttc cgccactttt tcgcccaacc
     2761 caacgattgg gcggaaaagt gcgagaagat cgcgccattt cgtcgatctc ggcgtgtgtc
     2821 ggaccgccgg gggacgatgc gacccatggt ggaagtattc cttggcaggg aagcacttgg
     2881 cgacggcctg cgtcgtcatg acctgcgtag ttggtatcgg ccgatattcc ggggtgttta
     2941 catcccgaaa gcgtcgacgc cgtcattgca cgacaggacg gtgggagcct ggctcacctc
     3001 ggaccgagca ggcgtcatcg ccggggtcgc cgcgtcggcg ctgcacggcg ccgcatgggt
     3061 cgacgacagc catccgattg agatcctggt cgacgaacga cgccgtcagc ccggtctcgt
     3121 cgtgcggatg gatcgcgtcg ccgacgatga gatcactatc gttgacgacc tcccggtgac
     3181 gactcccgcg cgcaccgcgt tcgataccgg tcgataccag cagcggtcgg tggcgctcgg
     3241 ccgtctggat gctctgatgc gcgcggcgcc gttcgcagac agagaagtgg cgacactgat
     3301 gcagcgatac ggtgcggtcc ggggcattcg ccagctgcgc gcgcttcttc cgctggtcga
     3361 cgccggcgcc gcgtcgctga aggagagttg gttgcgactt cttctgatcg acaacggatt
     3421 tccgattccc gaaacgcaga ttccggtgct cgacaacgat gagccgttcg cgtttctgga
     3481 catgggatgg cgtgaccttc tcctggcggt cgagtacgac ggcgaccaac atcgcaccga
     3541 tcgtcctcaa tacgtcaagg acgtcaggcg catcaagagg atcgaacggc acggctggga
     3601 gatcatccgg gtgatcaatg aagaccgtcc gcacgagatt ttggcgcaag tgtacgaggc
     3661 atggctccgc cgcggaggcg tcgagatcga cgaaatggcg gcaattactc gcactttccc
     3721 gcccaatcgt tggtttgggc gaaatgggaa tgcggcttag ggccttgggt tagtccgctg
     3781 cggagacgac gccgctgacc cgccggcctt ttgacaccga gttcagcacc acgagcatgg
     3841 cgtcggcgct ctgacgccag gagaactccg cactgcggat ttgtgccttg ccgccgagtt
     3901 gatcgcgcag caccggatcg gtcagcagcc gctcgagggc gacgaccatg tcgcgatggt
     3961 cgtcgacgag gatgccggtc acgccgtcga cgatcgagtc ggtgagcccg ccggaggacc
     4021 ggtagccgat cgtcggcacg ccgtgctggg cggcctcggt gaccgcgagg ccccaaccct
     4081 ccttgcgtga cggcagcaca tgcacccagc accgttgcag cacttcgtgt ttgctgacgt
     4141 catcgacatg accgtggaag gtgaccgcat cggcaacccc gagcagccgt gcgtgctcga
     4201 cgagacgatc gcgccaccag ccgccgccga cgatgtcgag atgcaggccg ggaatgcgcg
     4261 gcagcagttc ggcgactgcg ttcagcgcgt cctcgatctg cttgtggggc accagcctgc
     4321 tgagcagcgc gatgcgcgga tgctccgcgc gcgcaagcgc gagcgtctcc ggcggcgcct
     4381 cgtcgagtcc gttgcggacc accgcgatct ggccggcgtc gacaccgagg tcggtcaggt
     4441 cgcgcgccga cggcagcgac accgtgacgt actggttgcg tcggtgcagc catggtgaca
     4501 gccgtgactc gacgaaccag ccgaagctgc ccttccatcg accggcgacg ggccagagtt
     4561 cgcggtggca atggtgaacc aggacgacga cgcgacgacc gtaggcgagg cgggcaagga
     4621 agggcagacc gttctgggtg tcgatcacca cgtcggggcg gaccccgcgc agagggccga
     4681 ggccgacgcg ggccagcacc atcgccaacc ccgcccagat gtagatcgaa taggcgccac
     4741 cggcgcggtt gatccggacc ccgtcgacga cctcgttgcg cggggcaccg gcgtagcgcg
     4801 ccgtccgcag cgtcacgtcg acacccgatg cggccagctg cgcaccgat
//
LOCUS       DASYLX010000183         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_886134,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000183 DASYLX010000000
VERSION     DASYLX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886134"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VGP27_04440"
     CDS             complement(<1..1578)
                     /locus_tag="VGP27_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV7850601.1"
                     /translation="MTAGVVAPGTGRHEALPAASSFTGVGSLIRLALRRDRVRLSVWT
                     AALTLMMAYAPNAIKLAYPEEAQRQARVDLLRSPAGMMLGGPMFGVNETDLGVMMANE
                     LMLTLVIAASILSILTVIRHTRTEEESGAAELVMSSVVGRYARTYAALIVVGGVNAVL
                     AVTMTLAMASTGFGVVDTAAMCLGVTGVSMVFGAVAAVTAQLWRTARAATGAAMGVLA
                     MAALVRGVGDVIDNSGSALSWFSPIAWAQQMRPFVDLRWWPFAMLVALAVGLMALASA
                     LEGSREYDDGVVPSSGEHPNAHSIKSVFGLHLRLQRGLTIGWAVGLLLSGLAFGSMTK
                     SLLDAAGGNELLQRVLAQQGTDGVYTTLTQFLAAATTAYVVSAVLRVYTDEESGLGEA
                     VLAGAVSRWRWLLTAVAAATAGSAVLMFFAGVGMGLGAGTTIGDPQTIWRLTVAGLAF
                     VPAMAAMAGVAALAVALRHPWIGWLAVTFVIISLYLGALLRLPQWLLDASPIGQTKAP
                     IEYSGGALTALVVAAAAL"
     gene            complement(1575..2513)
                     /locus_tag="VGP27_04445"
     CDS             complement(1575..2513)
                     /locus_tag="VGP27_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7850602.1"
                     /translation="MTATSLQRASTGEGTRPAVEIHGLVKTFGRTRALDGLDLTVTPG
                     DVTGFLGPNGAGKSTAIRVLLGLLRADSGTVRLLGGDPWKDAVALHRRIAYVPGDVTL
                     WPNLTGLQAIDFLARLRGDGAVDNRRRDELIERFELDPHKKARTYSKGNRQKVAIVAA
                     FSSDAELYILDEPTTGLDPLMERAFQACVEEVTYRGAAVLLSSHILAEVEKLCKSVTI
                     IRAGRAVRSGTLEQLRHLMRTTVTARTRSDGKKLQSTPFVHDLTIHDGHYVFSVDRND
                     LDRAMALLTELGIIDLTVAPASLEDMFLREYQGPAR"
     gene            complement(2485..3171)
                     /locus_tag="VGP27_04450"
     CDS             complement(2485..3171)
                     /locus_tag="VGP27_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850603.1"
                     /translation="MRSADDLTALARIRDAAIDQVGQHGFSVGLRAIAQAAGVSAALV
                     IHHFGSKEGLLRACDEHIAAQIYEAKSESLRSADPSTWLRQMAEIDSYAPMTAYLLRS
                     LQSGSALAKEFWRRMIDNAEEYLEEGVRAGTLKPSRDPKARARFLGMNNGGGLLLYLQ
                     MHDNPSDLRAVLRDYAEDMMLPALELYSNGLMADSSMYEAFLAQHEQGIPFSNSTEGD
                     HDDGDVPATG"
     gene            complement(3329..>3796)
                     /locus_tag="VGP27_04455"
     CDS             complement(3329..>3796)
                     /locus_tag="VGP27_04455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7850604.1"
                     /translation="GAGVSGLWLLSGMREDGVLTTIDVEPEHQRLAKQAFNEAGIGPS
                     RTRLISGRAQEVLTRLADESYDLVFIDADPADQPDFVVEGVRLLRPGGAIVVHRAALG
                     GRAGDAKAHDAEVTAVREAARLIAENEHLTPVLVPLGDGLLAAARDWPSRDED"
BASE COUNT          644 a         1288 c         1256 g          608 t
ORIGIN      
        1 cagcgctgcc gcggcgacca ccagtgcggt cagggcgccg cccgagtact cgatcggcgc
       61 tttggtctga ccgatcggcg acgcgtcgag cagccactgc ggtagccgca acaacgcgcc
      121 gagatacagc gagatgatca cgaatgtcac tgccagccaa ccgatccagg ggtgccgcag
      181 cgcaactgcg agcgccgcga cacccgccat ggctgccatt gcgggcacga aagccaggcc
      241 cgccactgtc agacgccaaa tggtttgcgg atcgccgatg gtggtgcccg cgccaagacc
      301 cattccgaca cctgcgaaga acatcaacac cgctgacccg gcggtggcgg ccgcgacggc
      361 ggtgaggagc caccgccacc gcgacaccgc gccggccagc acggcctcgc caaggccgga
      421 ctcctcgtcg gtgtagacgc gcagcacggc ggagacgacg taggcggtgg tcgccgcggc
      481 gaggaactgc gtcaacgtgg tgtatacacc gtcggtgccc tgttgggcga gtacgcgctg
      541 gagtagttcg ttgcccccgg ccgcgtcgag cagcgacttc gtcatcgagc cgaatgccaa
      601 gccggacaac aacagtccga cggcccagcc gatcgttagg ccccgttgca ggcgaaggtg
      661 caggccgaac accgacttga tcgagtgcgc gttcgggtgc tcgccggacg acgggacgac
      721 gccgtcgtcg tactcccggc tgccctccag cgccgaggca agtgccatga ggccgacggc
      781 gagcgcgacc agcatggcga acggccacca tcgcaggtcg acgaacggac gcatctgctg
      841 tgcccacgcg atgggggaga accaggacag tgcgctgccg gagttgtcga tcacatcgcc
      901 gacgccgcgc acgagcgccg ccatcgcaag cacgcccatt gcggccccgg ttgcggcgcg
      961 cgccgtccgc cacagctgtg ccgtgaccgc ggcgacggcg ccgaacacca tcgacacgcc
     1021 tgtgaccccg agacacatcg ctgcggtgtc gaccacgccg aacccggtag aggccatcgc
     1081 cagcgtcatc gtcactgcga gcacggcatt cacaccgccg accacgatca gggcggcata
     1141 cgtccgggcg tagcgaccca ccaccgatga catgacgagt tcggctgcac cgctttcctc
     1201 ttcggtgcgg gtatgccgga tgacggtgag aatggacaga atcgaggcgg cgatcacgag
     1261 ggtgagcatc aactcgttgg ccatcatcac gcccaagtcg gtttcgttga cgccgaacat
     1321 ggggccaccc aacatcattc ccgcagggct cctgagcagg tctacgcggg cctgccgctg
     1381 cgcctcctcc gggtaggcca gcttgatggc attgggtgcg tacgccatca tcagcgtgag
     1441 ggcggcggtc cacacgctga gccggacgcg gtcacggcgc agcgcgagcc tgatcaggct
     1501 gccgacgccg gtgaacgagg acgccgcagg cagagcttca tggcggccgg tgcccggcgc
     1561 gacgacgccg gcagtcatcg cgcgggcccc tggtactcgc gcaggaacat gtcttccagt
     1621 gaagccggcg cgacagtcag atcgatgatg cccagctcgg tcagcagcgc catcgcgcgg
     1681 tccaggtcgt tgcggtcaac cgagaagacg tagtggccgt catggatcgt taagtcgtgt
     1741 acgaatggcg tgctctggag tttcttgccg tcgctgcggg tgcgtgccgt gaccgtggtc
     1801 cgcatcaggt ggcgcagctg ctccagcgtg ccggaccgca cggcgcggcc ggcgcggatg
     1861 atcgtgaccg acttacagag tttctccacc tcggcgagaa tgtggctgga cagcagcacc
     1921 gcggcgccgc ggtaggtgac ctcttcgaca caggcctgaa aagccctctc catcaacggg
     1981 tccaggcccg tggtgggctc gtcgaggatg tagagctctg cgtcgctgga gaatgccgcg
     2041 acgatggcca ccttctgccg gttgcccttc gagtaggtgc gcgccttctt gtgcggatcc
     2101 agttcgaacc gctcgatgag ctcgtcgcgc cgtcggttgt cgacagcgcc atcgcctcgc
     2161 agccgcgcca agaagtcgat ggcctgcagc ccggtcaggt tcggccacag cgtcacatca
     2221 cccggtacgt aggcgatgcg gcgatgaagt gccaccgcat ccttccacgg gtcgccgccg
     2281 agcagtcgta ccgtgccgct gtcggcacgc agcagtccga gcaacacccg gatcgcggtg
     2341 gactttcccg cgccgttggg tccgaggaag ccggtgacgt cgcccggcgt aacggtcagg
     2401 tccagcccgt cgagagccct ggtccggccg aacgtcttga ccaggccgtg tatctcgaca
     2461 gcgggccggg tgccctcgcc ggtgctagcc cgttgcaggg acgtcgccgt catcgtgatc
     2521 tccttcagtc gaattgctga acgggatgcc ttgttcgtgt tgcgcgagaa acgcttcgta
     2581 catcgacgag tcggccatca ggccgttgga gtacagctcc agggcgggca gcatcatgtc
     2641 ctcggcgtag tcgcgtagca ccgcgcgcag gtccgacggg ttgtcgtgca tctgcaggta
     2701 gagcagcagg ccgccgccgt tgttcatgcc gagaaacctg gccctggctt tcgggtcgcg
     2761 gctcggcttg agggtgccgg cgcgaacgcc ctcttcgaga tactcttcgg cgttgtcgat
     2821 catgcgccgc cagaactctt tggccagcgc gctgcctgat tgcaggctcc gcaggaggta
     2881 ggccgtcatc ggcgcatagc tgtcgatctc ggccatttgg cgcagccagg tggaggggtc
     2941 agcagaacgc aatgactccg acttcgcctc gtaaatctgc gcagcgatgt gctcgtcgca
     3001 cgcccgcagc aggccttcct ttgatccgaa gtggtggatc accagtgcgg cgctgacgcc
     3061 tgctgcctgc gcgatcgccc gcaacccgac gctgaagccg tgctggccca cctggtcgat
     3121 cgcggcgtcg cggatccgcg cgagggccgt gagatcgtcc gctgaacgca tgtttagtac
     3181 gctaaacgcg cgttcagcac gccgtcaagg ggctaacggt caaaatcgcg taaaaaagcc
     3241 cgccacttct cctcgcgagc agacgcaaac tcgcgcaaat ctgcagctat cgatgcgatc
     3301 ttgtgtctgc tcgcgcaaag aaggtgagtt agtcctcgtc gcgggatggc cagtcgcggg
     3361 ccgcggccag cagcccgtcg cccagcggca ccagcaccgg cgtcagatgc tcattctcgg
     3421 cgatcagtcg cgcggcctcg cgcaccgcgg tcacctcggc gtcatgggcc ttggcgtcgc
     3481 cggcgcggcc gccgagcgcc gcccggtgca cgacgatcgc accaccgggc cgcagcaacc
     3541 gcacgccctc gacgacgaag tccggctggt cggcggggtc ggcgtcgatg aacaccagat
     3601 cgtaggactc gtcggccagc cgggtcagta cttcctgggc ccggccgctg atcagccgtg
     3661 tccgcgacgg cccgatgccc gcctcgttga acgcctgctt ggcgaggcgc tgatgctcgg
     3721 gttcgacgtc gatcgtcgtc agcacaccgt cctcgcgcat gccggagagc aaccacagcc
     3781 cgctgacacc ggctcc
//
LOCUS       DASYLX010000184         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_227495,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000184 DASYLX010000000
VERSION     DASYLX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227495"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="treS"
                     /locus_tag="VGP27_04460"
     CDS             <1..1247
                     /gene="treS"
                     /locus_tag="VGP27_04460"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEV7850605.1"
                     /translation="IFVDTEESNWTFDPVRKQFYWHRFFSHQPDLNYDNPAVQEAMID
                     VLRFWLDLGIDGFRLDAVPYLFEREETNCENLPETHEFLRHCRKVLDDEYPGRVLLAE
                     ANQWPADVVEYFGDADTGGDECHMAFHFPLMPRIFMAVRRESRFPISEILAHTPPIPE
                     MAQWGIFLRNHDELTLEMVTDEERDYMYTEYAKDPRMKANVGIRRRLAPLLDNDRNQI
                     ELFTALLLSLPGSPVLYYGDEIGMGDIIWLGDRDGVRTPMQWTPDRNAGFSKANPGQL
                     YLPPNQDPIYGYQSVNVEAQRDSSASLLNWTRTMLAVRRRHEAFAIGSFRELGGSNPS
                     VLTYVRELERDGRQDVVLCVNNLSRFPQPIELNLQHWTGYTPVEMTGYVEFPPIGQLP
                     YLLTLPGHGFYWFELKAAEEKS"
     gene            1244..2578
                     /locus_tag="VGP27_04465"
     CDS             1244..2578
                     /locus_tag="VGP27_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltokinase"
                     /protein_id="HEV7850606.1"
                     /translation="MSRPANLPWSEWLPEQRWYAGRTRTLSAVEPASVVALRDDLELV
                     LLDASYTNGSTDTYQVVVRWDAGPIEEYADVATIGADEGRTAYDAVYNPADAKYLLSL
                     IDSSAAVGDLTFSKEPDVTLPLDSAPRVSSAEQSNTSVVYEEEAVFKIFRRITAGTNP
                     DIELNRVLARAGNPHVARLLGSYETSSGGESSALGMVTEFAANSAEGWDMATASTRDL
                     FAEGDLYADEVGGDFAGESYRLGEAVASVHATLAAELGTATSQFPIDAMMERLAAAVA
                     AVPDLEQYVPLIEERYRKLAEDTISVQRVHGDLHLGQVLRTPERWLLIDFEGEPGAPL
                     EERRRPDSPARDVAGVLRSYEYAAYQRLMEQATDDDRSRQLAARAREWVDRNCASFCE
                     GYGAQSGSDPRDSGALLAAYELDKAVYETAYEARHRPTWVPIPLRSIARIVS"
     gene            2643..>3109
                     /locus_tag="VGP27_04470"
     CDS             2643..>3109
                     /locus_tag="VGP27_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7850607.1"
                     /translation="MADVDFCVIGAGFAGLAAALRLKQAGRSVALLEARDRVGGRTFT
                     ELRADGTWIDRGGAWIGPGQDRIRALMKEFDVGSYKQYADGEAMMVVNGKQYRYKGTV
                     PRTLNPFATVNLGTAMLELAKMCKTIPLEAPWDAGKADKWDRITVGQWLDHHVR"
BASE COUNT          539 a         1009 c         1064 g          497 t
ORIGIN      
        1 tcatcttcgt cgacaccgag gaatcgaact ggacgttcga cccggtgcgc aagcagttct
       61 actggcaccg gttcttctcc catcagcccg atctgaatta cgacaacccg gccgttcaag
      121 aagcgatgat cgacgtgctg cggttctggc tcgacctcgg catcgacggc ttccggttgg
      181 acgcggtgcc gtatctgttc gaacgcgaag agacgaactg cgagaacctg ccggagaccc
      241 acgagttcct caggcactgc cgcaaggtgc tcgacgacga gtatcccggc cgcgtcctgc
      301 tggccgaagc gaaccaatgg cccgccgatg tggtcgagta tttcggcgat gccgacaccg
      361 gcggcgacga atgccatatg gcgtttcact ttccgctgat gccgcgcatc ttcatggcgg
      421 tccggcggga gtcgcggttt ccgatctcgg agatcctggc ccatacgccg ccgataccgg
      481 agatggcgca gtggggcatc ttcctgcgca accacgacga gttgacgctc gaaatggtca
      541 ccgacgaaga gcgcgactac atgtacaccg agtacgccaa ggatccgcgc atgaaggcga
      601 acgtcggcat ccgccgccgg ctcgctccgc tgctggacaa cgaccgcaac cagatcgagt
      661 tgttcaccgc gctgctgctg tcgctgcccg gctcgccggt gctctactac ggtgacgaga
      721 tcggcatggg cgacatcatc tggctcggcg accgcgacgg tgtgcgcacc ccgatgcagt
      781 ggacgcccga ccgcaacgcc gggttctcga aggccaatcc cggccagctc tatctgccgc
      841 cgaatcagga cccgatctac gggtatcagt cggtgaacgt ggaggcccag cgcgacagct
      901 ccgcatcgct gctgaactgg acgcgcacca tgctggccgt gcgacgtcgc cacgaggcgt
      961 tcgccatcgg atcgttccgc gagctcggcg gctccaaccc gtcggtgctg acgtacgtgc
     1021 gggaactcga gcgcgacggc aggcaggacg tggtgctgtg cgtgaacaac ctgtcgcgat
     1081 tcccgcagcc gatcgagctg aacctccagc actggacggg gtacaccccc gtcgaaatga
     1141 ccgggtacgt tgagtttccg ccgatcggcc agttgccgta cctgttgacc ctgccgggcc
     1201 acgggttcta ctggttcgag ctgaaggcgg ctgaggagaa gtcatgagcc ggcccgcgaa
     1261 cctgccatgg tccgagtggc ttcccgagca gcgctggtat gcgggccgca cccgtacgct
     1321 gtccgcggtc gagcccgcga gcgtggtggc gctccgcgac gatctcgagc tggtgctgct
     1381 cgatgcctcc tacacgaacg gctcgacgga tacctatcaa gtggtcgtgc gctgggacgc
     1441 cggcccgatc gaggagtacg cggacgtcgc gacgatcggt gcggatgagg gccgcaccgc
     1501 ctacgacgcg gtgtacaacc cggcggacgc gaagtacctg ctgtcgttga tcgactcgtc
     1561 ggcggccgtg ggcgacctga cgttcagcaa ggagcccgac gtcacgctgc ctctcgactc
     1621 cgcaccgcgg gtgtcgagtg ccgaacagag caacaccagc gtcgtgtacg aggaagaggc
     1681 cgtcttcaag atcttccggc gcatcaccgc gggcacgaac ccggacatcg agctgaaccg
     1741 cgtcctggcg cgagcgggca acccccacgt ggcgaggctg ctcggctcgt acgagacatc
     1801 gtcgggcggc gagtcgagtg cgctgggcat ggtgaccgaa ttcgcggcca actccgccga
     1861 gggttgggac atggccaccg ccagcacgcg cgatctcttc gccgaggggg acctgtacgc
     1921 cgacgaagtc ggcggcgact tcgcgggtga gtcgtaccga ctcggcgagg ccgtcgcctc
     1981 ggtgcacgcc acgctcgccg cggagctcgg cacggcgacg agtcagttcc cgatcgacgc
     2041 catgatggag cggttggcgg ccgccgtcgc ggcggtgccc gacctcgagc agtacgttcc
     2101 gctcatcgag gagcgctacc gcaagctggc cgaggacacg atcagcgtgc agcgcgtgca
     2161 cggcgacctg cacctggggc aggtgctgcg gacaccggag cggtggctgc tgatcgactt
     2221 cgaaggcgag ccaggagcgc ccctcgagga acgcaggcga ccggactcgc ccgcgcgcga
     2281 tgtcgcgggc gtgctgcgct cgtacgagta cgccgcctat caacggctga tggagcaggc
     2341 caccgacgac gaccggtctc ggcagctggc ggcccgcgcg cgtgagtggg tggatcgcaa
     2401 ctgcgcctcg ttctgcgagg gctacggcgc gcagtcggga tccgacccgc gggactcggg
     2461 cgccctgctg gcggcctacg agctggacaa ggcggtttat gaaaccgcct acgaggcgcg
     2521 gcaccgacct acgtgggtgc ccattccgct gcgctcgatc gcgcgcatag tcagctgagt
     2581 gacgaccgcg taaaatttgc caaaaggctg cgcggggcct tcggtaggtg gaccatcacg
     2641 tcatggctga cgtggacttt tgtgttatcg gggcggggtt cgccggcctc gcggccgcgt
     2701 tgcggctgaa gcaggcgggg cgttcggtgg cgctgctgga agcccgagac cgggtcggcg
     2761 gccgcacatt caccgaactg cgcgccgacg ggacgtggat cgaccgcggc ggcgcatgga
     2821 tcggaccggg tcaggaccgg attcgtgccc tcatgaagga gttcgacgtc ggaagctaca
     2881 agcagtacgc cgacggcgag gcgatgatgg tcgtcaacgg caagcagtac cgctacaagg
     2941 gcaccgtccc gcggacgctg aacccgttcg cgacggtgaa cctcggcacc gcgatgctcg
     3001 agctggccaa gatgtgcaaa acgattccgc tcgaggcgcc gtgggacgcc gggaaggcgg
     3061 acaaatggga ccgcatcacc gtcgggcagt ggctcgatca ccatgtgcg
//
LOCUS       DASYLX010000185         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3518925,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000185 DASYLX010000000
VERSION     DASYLX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518925"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="frr"
                     /locus_tag="VGP27_04475"
     CDS             <1..544
                     /gene="frr"
                     /locus_tag="VGP27_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV7850608.1"
                     /translation="LFDAEEKMEKAVSVARDDLSSIRTGRANPGMFSRLSIDYYGSST
                     PITQLSSINVPEARLVVIKPYEQSQLKPIEDAIRNSDLGVNPGNDGNVIRVAIPQLTE
                     ERRRELVKQAKAKGEDARVSVRNIRRKAMEELHRIKKDGEAGEDEVKHAEKELDKTTH
                     TYTSHIDDLVKHKEGELLEV"
     gene            544..1413
                     /locus_tag="VGP27_04480"
     CDS             544..1413
                     /locus_tag="VGP27_04480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV7850609.1"
                     /translation="MADQQADSVADTSEPPKKASRAGRNLPAAIGVGVVLGAMAIGIL
                     LFKPIGWLPVLAVFIPIATYEVIRRLREAGYALPLIPLLLGGQAMIWLTYLGPAGVLG
                     AYGGTIVVAMVWRLVGQGIRAAPVNYLRDISATVLLATWVPLFAAFSALLIFQDHGGT
                     RTFIVIVTVVFADIGGYVAGVLFGKHLMVPAISPKKSWEGLVGSVVFGVTAAVLSVAF
                     LLDRPAWVGVPLGLMLVITGVLGDLVESQVKRDLGIKDMGTLLPGHGGIMDRIDAMLP
                     SAVAGWIVLTLLA"
     gene            complement(1563..3062)
                     /locus_tag="VGP27_04485"
     CDS             complement(1563..3062)
                     /locus_tag="VGP27_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7850610.1"
                     /translation="MYVRIMSAVQVREAMANLCAAYDAFDASELDALTRGELLSVMDD
                     LETLMCRMPTQSHRLLTRLQAETTPKQMGGKSWNEVLRIRWRLSSAEASRRLHDAAEL
                     GARKNLLGQPLEPVLPAVAAAQSAGLITGDHVTTIRDAIKRLPSWVDTITRAQFEVDL
                     VRVAAGVGPKELRETAALRLFLLDQDGPEPDDAERERKRRVSKGRQGRDAMTTLAADL
                     TPEAWAVWEVIFAKYAAPGMCNPADEEPCTSGTPSQAQIDNDHRTLEQRQHDAMLAVG
                     RIALMSGELGHLNGLPVSVIIRTTLQDLESRAGIGVTGGGTKLPIADVIKMGAHAYHN
                     LAVFDGVTGQALALFRTRRVASAAQRIMLIARDGGCTKPCCTVGAYGCQVHHASQDWA
                     NGGNTNVDDLTLACGTDNRGVDDGGWQTTINTRGECEWTPPSALDHGQSRINYYHRPE
                     ALLRPPEDRAAPEPTLLKRKRHNATHVKSLRLDPTETPGGPEPPDNQAA"
     gene            3135..4127
                     /locus_tag="VGP27_04490"
     CDS             3135..4127
                     /locus_tag="VGP27_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7850611.1"
                     /translation="MKLSCALVTNADSPDIVAEAERLGYHRAWLYDSPAITSDVWMAL
                     ALAAQRTSTIGLGPGVLVPSLRHPMTNAAAIATLAALAPGRVAVALGSGFTGRYILGK
                     RPLRWAFVEEYVRALRAMLRGETVQWEDAPIRMLHTEGFVADRPVEVEVLIGADGPKG
                     TAVAERVGDGVFAAGVPNPAAAGRPYSLLQFGTVLDGGEDLHSQRVLERAGHGLAVVF
                     HAMYERLGPDAVRGLPGGNEWVAAIEAIPPAERHLITHEGHLVRLTAVDTEAVALAAD
                     LLPQFTFSGTAEQLAARAAEYEAGGVTELVYQPAGPDVAGELARMMEAVGSSAS"
     gene            complement(4055..5008)
                     /locus_tag="VGP27_04495"
     CDS             complement(4055..5008)
                     /locus_tag="VGP27_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7850612.1"
                     /translation="MERMSLPTMPARTASILTFFYALGYPIGALAVSAMSPMAVLVFR
                     FGLAGGILSGWVFAARVGWPTGRTLIHVLVSGLLGQALQFICLYVALLHGAPAVLGAV
                     IISMNPVVTTVLAALFLGESLTWGRAIALLLGVVAVLAACAGRLVAVGGIDAVVLLLV
                     VALLAVAAGGVYQQRFCSDVDFRAMAALQNLVSLVPVAALLFVSPWSVTDPWKAVWAV
                     AAVVLFNATTAMTLYVRAINNYGAAAVAMLFAVIPAVAGLLSWLMLGQRPDIGIAVGL
                     VVGAAACWLNARVSRRQRQDADEPTASIMRANSPATSGPAG"
BASE COUNT          870 a         1654 c         1685 g          874 t
ORIGIN      
        1 cctcttcgac gccgaggaga agatggagaa ggcggtgtcc gtcgcgcgcg acgacctgtc
       61 gtccatccgg accggccgcg ccaaccccgg catgttctcc cgcctctcga tcgactacta
      121 cgggtcgtcg accccgatca cgcagctgtc cagcatcaac gtgccggagg cgcggctcgt
      181 ggtgatcaag ccgtatgagc agtcacagct gaagccgatc gaggacgcca ttcgcaattc
      241 cgacctcggc gtcaacccgg gcaacgacgg caacgtcatc cgcgtcgcca ttccgcagct
      301 caccgaggaa cgccgccgcg agctcgtgaa gcaggccaag gccaagggag aggacgcccg
      361 agtgtccgtg cgcaatatcc gtcgaaaggc gatggaggag ctgcaccgga tcaagaagga
      421 cggcgaggca ggcgaggacg aggtcaaaca cgccgagaag gaactcgaca agaccaccca
      481 cacctacacc agccatatcg acgaccttgt caagcacaaa gagggcgaac tgctggaggt
      541 ctgatggccg atcagcaagc agattcggtg gccgacacgt cggagccgcc caagaaggca
      601 tcccgggccg ggcgcaatct cccggcggct atcggcgtgg gcgtagtgct cggcgcgatg
      661 gccatcggca tcctgctgtt caagccgatc ggctggctgc ccgtgctggc ggtgttcatc
      721 ccgatcgcca cctacgaggt catccggagg ctgcgcgaag ccggctacgc gcttccgctc
      781 attccgctgc tacttggcgg ccaggcgatg atctggctga cgtatctcgg gccggcgggg
      841 gtcctgggcg cctacggcgg cacgattgtc gtagccatgg tctggcggct ggtcggacag
      901 ggcatccgcg cggcacccgt caactacctg cgcgacatct cggccaccgt actgctggcg
      961 acatgggtgc ccctgttcgc cgccttcagc gcgctgctga tcttccagga ccacggcggc
     1021 acccggacgt tcatagtgat cgtgaccgtc gtcttcgccg acatcggcgg ctacgtggcc
     1081 ggggtgttgt tcggcaagca cctgatggtg cccgcgatca gcccgaagaa gtcgtgggag
     1141 ggactggtcg gctcagtggt cttcggtgtc acggcggcgg tgctgtcggt ggccttcctg
     1201 ctcgaccggc ccgcatgggt cggtgtgccg ctggggctga tgctcgtcat caccggcgtg
     1261 ctgggtgact tggtcgaatc tcaggtcaag cgcgatctcg gcatcaagga catgggcacg
     1321 ctgcttcccg gacacggcgg catcatggac cggatcgacg cgatgctgcc gtcggccgtc
     1381 gccggctgga ttgttctgac gctgctggca taggcacgcg taatcgacgg tggacgcgcg
     1441 ggatcctgcg gtgtggccgc tggttgacgt gcaatacgtt gtcggccagg cagttcggag
     1501 ttcagattct gtggtgcgcc gcggacgagt ttcgtgatgt gccatccact ggtgtgtggg
     1561 cgctaggcgg cttggttgtc aggtggttcg gggccgccgg gtgtttcggt ggggtcgagt
     1621 cttagtgact tgacgtgggt agcgttgtgc cgcttgcgtt ttagcagtgt cggttcgggt
     1681 gctgctctgt cctcgggtgg cctgagtagc gcttccggac ggtggtagta gttgattctg
     1741 gattggccgt ggtcgagggc agatggtggt gtccattcgc attcaccgcg ggtgttgatg
     1801 gtggtctgcc agccgccgtc atcgacgccg cgattgtcgg tgccacacgc cagggtcagg
     1861 tcgtcgacgt tggtgttacc gccgttggcc caatcctgag acgcgtggtg gacctggcag
     1921 ccgtaggccc ccacggtgca gcacggtttg gtgcagccgc cgtcgcgggc gatcagcatg
     1981 atccgctggg cagccgaggc gacgcgtctg gtgcgaaaca gcgccagcgc ctgaccggtc
     2041 actccgtcga acaccgcgag gttgtggtag gcatgagcac ccattttgat gacgtcggcg
     2101 atcggcagtt tggttccccc acctgtgaca ccgatgccgg cgcggctttc caggtcttgc
     2161 agggtggtgc ggatgatgac gctgaccggg agcccgttga ggtggccgag ttcaccgctc
     2221 atcaacgcga tgcgaccgac cgccagcatc gcgtcgtgct gccgctgctc cagcgtgcgg
     2281 tgatcgttgt cgatctgcgc ctgcgacggg gtgcccgagg tgcacggttc ctcatcggcg
     2341 ggattgcaca tccccggcgc ggcgtacttg gcgaagatca cctcccacac cgcccacgcc
     2401 tccggcgtca gatcggcggc cagcgttgtc atcgcgtcgc gcccttgccg acccttcgag
     2461 accctgcgtt tgcgttcacg ttcggcgtca tcaggttcgg gaccgtcctg atccagcagg
     2521 aacaaccgca gcgctgccgt ctctcgcaac tccttgggcc cgacgccggc cgcgacccgc
     2581 accaggtcca cctcgaactg cgcacgggtg atcgtgtcga cccaggacgg caggcgcttg
     2641 atggcgtccc ggatggtcgt aacgtggtca ccggtgatca atcctgccga ctgagcggcg
     2701 gcgacagcag gcagcaccgg ctccaacggc tggccgagca ggttcttacg ggctcccagt
     2761 tcggccgcgt catgcagccg ccgtgacgct tctgccgagg agaggcgcca gcggatccgc
     2821 aatacctcgt tccatgattt gccacccatc tgcttgggcg ttgtctccgc ctgcagtcgg
     2881 gtcagcagcc gatgcgactg ggtgggcatt cggcacatca gcgtctcgag atcgtccatc
     2941 accgagagca actctccgcg agtcagtgcg tccagttcgg aggcatcgaa ggcgtcgtaa
     3001 gcggcgcaca agttggccat cgcttcccgc acttgcaccg ctgacatgat tcgaacatac
     3061 attcgactac cgacaacaag ctatcaggcc gtgccttgat cgaggccggg agcgcgccgg
     3121 taggttctac gcgcatgaag ctttcgtgcg ccttggtcac caacgccgat tcgcccgaca
     3181 tcgttgctga ggcggaacgg ctcggctacc acagagcttg gctttacgac tcgcccgcga
     3241 tcacctcaga tgtgtggatg gctttggcgc tggcggcgca gcgcacgtcg actatcggac
     3301 tcggacccgg cgtactcgtg ccgagcctgc gccacccgat gacgaacgcg gccgcgatcg
     3361 cgactcttgc cgcgctcgcg cccggtcggg tcgcggtggc tctcggatcg ggtttcaccg
     3421 gtcgctacat cctcggaaag cggcctctgc gttgggcatt cgtcgaggag tacgtcagag
     3481 cactgcgcgc aatgctgcga ggcgaaaccg tgcagtggga agacgccccc attcgcatgc
     3541 tgcacacgga agggttcgtc gccgaccgtc ccgttgaggt cgaggtgctc atcggagccg
     3601 atggcccgaa gggcaccgct gtcgctgaaa gggtgggcga cggggttttc gccgcgggcg
     3661 tgcccaaccc cgcggcggca ggccggccat actcgctgct gcagttcggc accgtgctcg
     3721 acggcggcga ggacctacac agccagcgcg tgctggagcg cgccgggcac gggttggcag
     3781 ttgtcttcca cgcgatgtac gagcgcttgg gtccggacgc cgtgcgcggg ctgccgggtg
     3841 gcaacgagtg ggtggcggcg attgaagcga tcccgcccgc cgagcgtcat ctcattaccc
     3901 acgaaggcca tctcgtccgg ctgaccgcgg tggacaccga ggcggtcgcg ctagccgccg
     3961 acctgcttcc gcagttcacg ttcagcggga cagccgagca gttggccgcc cgcgccgccg
     4021 aatacgaagc cgggggcgtc accgagctgg tctatcagcc cgccggcccc gacgtcgcgg
     4081 gcgaattggc gcgcatgatg gaggccgtcg gttcgtctgc gtcctgacgt tgtctgcgcg
     4141 acacgcgcgc attgagccaa caggctgctg cgccgaccac caggccgacc gcgatgccga
     4201 tgtcgggccg ctgacccaac atgagccagg acagcaggcc ggccacggca gggatcaccg
     4261 cgaaaagcat ggcaactgcc gctgcgccgt agttgttgat ggcgcgcaca tagagcgtca
     4321 tcgccgtcgt cgcattgaac agcaccactg ccgccaccgc ccaaaccgct ttccacggat
     4381 cggtcaccga ccacggcgac acgaacagca gcgccgccac cggaaccagg gacacgaggt
     4441 tctgcagcgc ggccattgca cgaaagtcga cgtcggagca gaaccgctgc tggtagacac
     4501 cgccggctgc gaccgccaac agggcgacca ccagcagcaa caccacggcg tctataccgc
     4561 cgacggcgac caatcggcct gcgcatgcgg caagcaccgc gaccacgccg agcaggagcg
     4621 cgattgcccg accccaggtg aggctctcac cgaggaacaa agctgcaagc accgtcgtga
     4681 ccaccgggtt catcgagatg atcactgctc ccaacaccgc gggagcaccg tgtaacagag
     4741 ccacgtacag gcagatgaac tgcagtgcct ggccgagcag cccgctcacc aggacgtgaa
     4801 tcagcgtgcg gcccgtcggc cagcccacgc gcgcggcaaa cacccatccg gacaggatgc
     4861 cgcccgccaa tccgaaccgg aataccagca ccgccatcgg tgacatagcc gaaacggcaa
     4921 gggcaccaat cgggtaaccc aacgcgtaga agaaagtcag gatggacgct gtccgggcgg
     4981 gcatcgtcgg caggctcatg cgctccatgg ttgcggccaa cgcaactcag cgccaatcaa
     5041 tttccgcctg ctacggatga gatactgaca aaagctcatg tca
//
LOCUS       DASYLX010000186         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_776810,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000186 DASYLX010000000
VERSION     DASYLX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776810"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGP27_04500"
     CDS             <1..253
                     /locus_tag="VGP27_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HEV7850613.1"
                     /translation="FVLVSAGVIILRRNRPDLHRAFRTPLVPWLPIAAIVACVWLMLN
                     LTALTWIRFLVWMAIGMAVYLLYGRRHSALAQRESAISA"
     gene            334..1191
                     /locus_tag="VGP27_04505"
     CDS             334..1191
                     /locus_tag="VGP27_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEV7850614.1"
                     /translation="MLNEHLRRHDGVLTLADARRCGIDRHAVHRRVRAGHWRQCGRGV
                     YFVDDRPFTDAARVRAAVWSYGERAAASGLAAAFWLDLTRFPPAIVEVTVPRDSNGRK
                     RAGSRVRRRDLAAADVVERRGLRVTAFPLTVVEASVRERGGAKLMDSALQRDVTLREM
                     WAAHLRNKGRYGSPASRLLLQAASDGARSSAERLLVTLLRDAGITGWQTNYPVGGYKV
                     DVSFPGAKVAIEVDGFAFHSGPVEFQIDRERQNNIALLGWQVLRFTWLDLTEYPQRVL
                     AVIRSATSA"
     gene            1297..>1678
                     /locus_tag="VGP27_04510"
     CDS             1297..>1678
                     /locus_tag="VGP27_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEV7850615.1"
                     /translation="MTQDNGVLTQLEIDQWRDKKRHLWLMGLIAPTALFVMLPLVWAF
                     NHWGWHTAAQVPFWIGPILLYILLPALDLRFGPDGQNPPDEVMERLENDKYYRYCTYI
                     YIPFQYASVVFGAYLFTASDLTWLG"
BASE COUNT          273 a          541 c          549 g          315 t
ORIGIN      
        1 gttcgtgttg gtctcggcgg gcgtcatcat ccttcggcgc aaccgtcccg acctccaccg
       61 cgccttccgg acgccgctgg tgccctggct gccgatcgcc gcgatcgtcg cctgcgtgtg
      121 gctgatgctg aacctgacgg cgctgacctg gatccggttc ctggtgtgga tggcgatcgg
      181 catggccgtc tacttgctct acggccgtcg ccattcggcg ctggcccagc gggagtctgc
      241 gatttcggcg tgactggttg cgctcagcgc aaccggccac gccgaaatca cggtaggggg
      301 aacctttccg gcgcggcgcg catctgccat tgagtgctga acgaacatct gcggcgacac
      361 gacggagtgt tgacgctcgc cgacgcccgt cggtgtggaa tcgacaggca tgccgtccac
      421 agacgggtcc gagcgggaca ttggcggcag tgtggccgcg gcgtgtactt cgtcgacgac
      481 cgcccgttta ccgacgccgc ccgcgtccgc gcagccgtgt ggagttacgg cgagcgtgcc
      541 gccgccagcg gactcgccgc ggccttctgg ctcgacctga ccaggttccc gcccgccatc
      601 gtcgaggtga cggtgccgcg cgacagtaac ggccgcaagc gtgccggttc acgtgtccgt
      661 cgacgagatc tggccgctgc agacgtcgtc gagcggcggg gtctgcgcgt aacggcgttc
      721 ccgctgaccg tcgtcgaagc gtcggtgcgg gaacgcggcg gcgccaaact gatggactcg
      781 gccctacagc gcgacgtcac gctgcgtgag atgtgggccg ctcacctgcg caacaagggg
      841 cgatacggat ctccggcgtc gcggctactt ctccaggccg cctccgacgg cgcgcggtcg
      901 tctgccgagc gactgcttgt gacgttgttg cgcgacgccg gtatcaccgg ctggcagacg
      961 aactatccgg tcgggggata caaggtcgac gtcagttttc ccggcgcgaa agtagcaatc
     1021 gaagtcgacg ggttcgcatt tcacagcgga ccggtcgagt ttcagatcga tcgcgaacga
     1081 cagaacaaca tcgccttact cggctggcag gtgctgcgct tcacctggct cgatctcacc
     1141 gagtacccgc agcgggtgtt ggcggtcatt cgctcagcga cttcggcgtg aaatggtgcg
     1201 ctcagcgcga cgggacacgc caaaatcgct gactgggtgt acaaagcggg catatttgtc
     1261 tagacagccg acaaaccggc ctctatagtc aacgtcatga cccaggacaa cggcgtactg
     1321 acgcaactcg agatcgatca gtggcgggac aagaagaggc atctgtggct gatggggctg
     1381 atcgccccga ccgccttgtt cgtgatgctg ccgctggtgt gggcgttcaa tcactggggc
     1441 tggcacaccg cggcgcaggt gccgttctgg atcggcccaa tcctgctcta catcctgctc
     1501 cctgcgctgg acctgcgctt cggccccgac gggcagaacc cgcccgacga agtgatggag
     1561 cggttggaga atgacaagta ctaccgctac tgcacctaca tctacatccc gttccagtac
     1621 gccagcgtgg tattcggcgc atacctgttc accgcgtccg acctcacttg gttgggct
//
LOCUS       DASYLX010000187         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2421893,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000187 DASYLX010000000
VERSION     DASYLX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421893"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGP27_04515"
     CDS             <1..301
                     /locus_tag="VGP27_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEV7850616.1"
                     /translation="LAYTTIMTVLQRLAKKNLVVQHRDDRAHRYAPTHGRDELVAGLM
                     VDALDQAADSGSREAALVHFVERVGADEAAALRRALAELETKHEMPPSSGGAGSP"
     gene            316..1236
                     /locus_tag="VGP27_04520"
     CDS             316..1236
                     /locus_tag="VGP27_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV7850617.1"
                     /translation="MSALAFTLVALLLVGPVPALLARATWPMRAPRASIVLWQSIALA
                     AVLSAFSAGIAIASRLFVPGADGRPTATITSEIEVLGWPLWMTYVLVFALTLLIGARL
                     MVAVVQVAIATRRRRAHHRMVVDLVGKSQKSGLRVLDVAQPIAYCLPGVRSRVVVSEG
                     TLTTLADKEITAILSHERAHLRARHDLVLEMFTAVHAAFPRFVRSANALDAVRLLIEM
                     LADDAAVRAAGPTPLARALVACAAGRTPAGALAAGGPTTVLRVRRLGGRGNSHAAAIG
                     AYLAAVAVLVTPTVALAVPWLTELHRLFIA"
     gene            1291..2748
                     /gene="gndA"
                     /locus_tag="VGP27_04525"
     CDS             1291..2748
                     /gene="gndA"
                     /locus_tag="VGP27_04525"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV7850618.1"
                     /translation="MSSSESTNGTAQIGVTGLAVMGSNIARNFAHHGYTVALHNRSVA
                     KTDTLLDEHGSEGTFVRAETMADFAAALQKPRRALIMVKAGDPTDAVINELAEVFEEG
                     DIIIDGGNALYTDTIRREKAMRERGLHFVGAGISGGEEGALKGPSIMPGGPAESYKTL
                     GPLLEEISAHVDGVPCCTHIGPDGAGHFVKMVHNGIEYSDMQLIGEAYQLLRDGLGLE
                     AGQIADVFGEWNKGDLDSFLIEITAEVLRQTDAKTGKPLVDVILDEAEQKGTGRWTVK
                     SALDLGVPVTGIAEAVFARALSGSVTQRKATTGLASGALGEKPGDASQFIDDVSKALY
                     ASKIIAYAQGFNQIQAGSAEYDWGITPGDMATIWRGGCIIRAKFLNRIKEAFDDEPDL
                     ATLIVAPYFRDAIESAIDSWRRVVVTATQLGIPIPGFASALSYYDALRTERLPAALTQ
                     GLRDFFGAHTYGRIDADDDKRFHTLWSGDRSEVPA"
     gene            2830..>3347
                     /locus_tag="VGP27_04530"
     CDS             2830..>3347
                     /locus_tag="VGP27_04530"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV7850619.1"
                     /translation="MRFLDGQKPAYDLTYSDVFIVPGRSDVASRFDVDLSTSDGSGTT
                     IPVVVANMTAVAGRRMAETIARRGGIVVLPQDLPISAVEETVNFVKSRDLVVDTPVTL
                     LPDDSVSDATALIHKRAHGAAVVVFEGRPVGLVTEANCAGVDRFARVRDVAITDFVTA
                     PVGTEPRKVFDLL"
BASE COUNT          548 a         1164 c         1099 g          536 t
ORIGIN      
        1 cctggcctat acgacgatca tgaccgtgct tcaacggttg gccaagaaga acctcgtggt
       61 gcagcatcgc gacgaccgcg cccaccggta tgcgccgacg cacggccgcg acgaactcgt
      121 cgcgggactg atggtcgacg cgctcgacca ggccgcagac tccggtagcc gggaggccgc
      181 gctcgtgcac ttcgtggagc gggtcggtgc cgatgaggcg gccgcactcc gacgggcgct
      241 cgccgagttg gaaaccaaac acgaaatgcc cccatccagt ggcggtgcgg gctctccctg
      301 agggacactg acagcgtgtc cgccctggcc ttcaccctcg tcgcgctgct tctggtcggg
      361 cctgttccgg ccttgctggc ccgtgcgaca tggccgatgc gggcaccacg ggcctctatc
      421 gttttatggc agtcgatcgc cctggcggcg gtgctgtcgg cgttcagcgc gggcatcgcg
      481 atcgcaagca ggctgttcgt gcccggcgcc gacggtcggc cgacggccac catcacgagc
      541 gagatcgagg tgctcggctg gccgctgtgg atgacctatg tcctcgtctt cgcgctcacc
      601 ctgctgatcg gcgccaggct catggtcgcc gtggtgcagg tggcgatcgc gacccgacgc
      661 cgaagggcgc accaccgcat ggtcgtcgac ctggtgggca agtcgcagaa aagcgggctg
      721 cgcgtgctcg atgtggccca gcccattgcg tactgcctcc ccggtgtgcg cagccgcgtc
      781 gtcgtcagcg agggcacgct gaccaccttg gcggacaagg agatcaccgc catcctgtcc
      841 cacgagcgcg cccacctgag ggcccgtcat gacctggttc tggagatgtt caccgcggtg
      901 cacgccgcgt tcccgcgctt cgtccgaagc gccaacgcgc tggatgccgt gcggcttctc
      961 atcgagatgc tcgccgacga cgccgcagtg cgcgcggccg gccccactcc cctggcccgg
     1021 gcgctggtcg cctgcgcggc gggacgaacc ccagcgggcg ctctggcggc gggcggaccg
     1081 acgaccgtgc tgcgggtgcg ccgtctcggc ggtcgcggca acagccacgc cgcggcgatc
     1141 ggcgcgtatc tcgccgccgt ggctgtgctc gtcacgccca ctgtcgcgct ggcagtgccg
     1201 tggttgaccg agcttcaccg attgttcatc gcctaggcgg cagttcgcca aacaccctgt
     1261 gcgacaacaa aacaacgaaa ggcctcgtct gtgagctcgt ctgaatccac caatggcacc
     1321 gctcagatcg gcgttaccgg ccttgccgtc atggggtcca acatcgcgag gaacttcgcc
     1381 catcacggct acaccgtcgc actgcacaac cgttctgtgg ccaagaccga cacactgctg
     1441 gacgagcacg gctccgaagg cacgtttgtg cgtgccgaga cgatggccga tttcgcggct
     1501 gcactgcaga agccgcggcg cgccttgatc atggtcaagg cgggcgaccc gaccgatgcc
     1561 gtcatcaacg aactcgccga ggtcttcgaa gagggcgaca tcatcatcga cggcggaaac
     1621 gcgttgtaca ccgacaccat tcgccgggag aaggcgatgc gggaacgcgg cctgcacttc
     1681 gtcggtgcag gcatctcggg cggcgaggag ggcgcgctga agggcccgtc gatcatgccg
     1741 ggcggccctg cggagtcgta caagacgttg ggcccgttgc tcgaggagat ctccgcgcac
     1801 gtcgacggcg tgccctgctg cacccacatc gggcccgacg gcgcaggcca cttcgtgaag
     1861 atggtgcaca acggcatcga gtactccgac atgcaactca tcggcgaggc ctaccagctt
     1921 ctgcgcgacg gccttggcct ggaggcgggc cagatcgccg acgtgttcgg cgaatggaac
     1981 aagggcgacc tcgacagctt cctgatcgag atcaccgccg aggtgctgcg tcagaccgac
     2041 gccaagaccg gcaagccgct cgtcgacgtc atcctcgacg aggccgagca gaagggcacc
     2101 ggccgctgga cggtcaagtc cgccctcgac ctgggcgtgc ccgtcaccgg catcgccgag
     2161 gcggtgttcg cacgtgcgct gtccggatcg gtgacccagc gcaaggccac caccggactg
     2221 gcatcaggcg ccctcggcga aaaacccggc gatgcaagcc agttcatcga cgacgtcagc
     2281 aaggcgctct acgcttcgaa gatcatcgcc tacgcccagg gcttcaacca gatccaggcg
     2341 ggcagcgccg agtacgactg gggcatcacg cccggcgaca tggccaccat ctggcgcggc
     2401 ggctgcatca tccgcgcgaa gttcctcaac cggatcaagg aggccttcga cgacgagccg
     2461 gatctggcga cgctgatcgt cgcgccgtac ttccgcgacg ccatcgaatc cgcgatcgac
     2521 agctggcgcc gcgtcgtcgt cacggccacg cagctgggca tcccgatccc cggcttcgcc
     2581 tccgcgctgt cctactacga cgcgctgcgc accgagcggc ttcccgccgc gctcacccag
     2641 gggctgcggg acttcttcgg cgcgcacacc tatggacgca tcgacgccga tgacgacaag
     2701 cgcttccaca cgctctggag cggggaccga agcgaagtgc ctgcttaacg ccgggcatgc
     2761 gggcagatgt gacttcgcgc cattgagcga tctgtccgca ctctcggggg aaaactagac
     2821 tggtcggcga tgagattctt ggacggccag aagccggcgt atgacctgac ctacagcgac
     2881 gtgttcatcg tcccgggtcg gtcggatgtg gcgtcccggt tcgacgtcga cctgtcgact
     2941 tcggacggct cgggcaccac catccccgtc gtcgtggcca acatgaccgc cgttgccggg
     3001 cggcggatgg ccgagacgat agcgcgtcgc ggcggcatcg tggttctgcc gcaagacctt
     3061 ccgatctcgg cggtcgagga gacggtgaac ttcgtcaaga gccgggacct cgtcgtcgac
     3121 accccggtga cgttgttgcc cgacgattcg gtgtccgacg cgacagccct catccacaaa
     3181 cgcgcgcacg gcgcagcggt ggtggtcttc gagggcaggc ccgtcggcct ggtcaccgag
     3241 gccaactgcg ccggcgtcga ccgcttcgcc cgcgtgcgcg acgtcgctat caccgacttc
     3301 gtcaccgcac ccgtcggcac cgagccgcgc aaggtcttcg acctgct
//
LOCUS       DASYLX010000188         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2202452,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000188 DASYLX010000000
VERSION     DASYLX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202452"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VGP27_04535"
     CDS             complement(<1..861)
                     /locus_tag="VGP27_04535"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7850620.1"
                     /translation="MTTAVSAYAATSATEPLTKTTITRRDVGPRDVAFDIHFAGICHS
                     DIHTVRAEWGDVTYPIVPGHEIAGIVTEVGSEVTKYKVGDRVGVGCFVDSCRECEYCL
                     AGEEQYCNNPGMVGTYNGVGRDGQPTQGGYSGAIVVDEDYVLRVPDSISLDAAAPLLC
                     AGITTFSPLRHWNAGPGTKVAVIGLGGLGHLAVKLAVALGAEVTVLSQTLKKMEDGLR
                     LGAAEYYATSDPETLEKLAGQFDLILNTVSANLDMGAYLGLLAVDGTLVELGAPEQPL
                     TVPFFPLAGGR"
     gene            complement(988..1263)
                     /locus_tag="VGP27_04540"
     CDS             complement(988..1263)
                     /locus_tag="VGP27_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7850621.1"
                     /translation="MIHVLRSTYLQSAEVVEQTRPAHLQFLKEEIAAGRLLLAGRQDN
                     GSGGMLITADIAVEQADDMIARDPYTKAGVANYERTSFNGALRAPGL"
     gene            complement(1274..1951)
                     /locus_tag="VGP27_04545"
     CDS             complement(1274..1951)
                     /locus_tag="VGP27_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850622.1"
                     /translation="MPLARAGRPRLHTQRRPGMNARDEILDAAGELFTTLGYAGTSTR
                     SIAESVGIRQASLYHYFNTKDDILCALLSQTVAPTLSFISDLLDNDPALTAAEYLHAL
                     AAFDGDRLLSGRWNLGALYLLPELRDARLEPFWSQRERLRLHYLAHSHSVVKQTGVNQ
                     AAADLPFHLVESLVNMWSVAPGPERASLPFHVADACMRVLGITDNDNSALHERSRLEI
                     QRYAVAE"
     gene            2169..>3794
                     /locus_tag="VGP27_04550"
     CDS             2169..>3794
                     /locus_tag="VGP27_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7850623.1"
                     /translation="MGIAISEADARTAVAGVPPTADPVATVGDLRVTFRRNGRDVHAL
                     RGVSLSIAAGEILGLVGESGSGKSVLGFSVLGLLPAAAHIEGAVRVSGADMVRGGAKA
                     LRKVRALDLGAVFQDPMSSLNPTMRIGKQVAEAAGSDAEALRLLTAVGIPEPKRRMRA
                     YPHELSGGLRQRVMIAIAVAGDPELIIADEPTTALDVTVQAQVLRLLRRLRDEIGCSI
                     LLITHDLGVAAQISDRIAVLYAGRIAEIGPTAAVLQTPAHPYTHGLLRSRLTLDTHRD
                     RRLAALAGTVPSPVTPLPGCAFAPRCALAAEDCSKTPPDPIAVGADRVSACIFGLDKV
                     AGEMGTKSTNTSQPFPEQADHDEEPPSVVMRDVTKTFSVAKRWLSRSTGNHGKLQALR
                     GVSLRVGHGESVALVGESGSGKSTLLRIIAGLEKPTWGSVQLAGEQRPQMVFQDAGAS
                     LTPWMSVGELIAERLRGSGVARAARRDAVAEVLELVGLSPDIAKSRAGQLSGGQRQRV
                     SLARATVVPPSVLLCDEPTSALDVSLAASVLNLI"
BASE COUNT          632 a         1226 c         1290 g          646 t
ORIGIN      
        1 gcgtccaccc gcgagcggga agaacggcac ggtcagcggc tgctcgggcg cgccgagctc
       61 gaccagggtg ccgtcgacgg caagcaggcc gaggtaggcg cccatgtcga ggttggccga
      121 caccgtgttc aggatcaggt cgaactgtcc cgccagcttc tctaacgtct cagggtcgct
      181 ggtggcgtag tactcggccg caccgagccg caggccgtcc tccatcttct tcagcgtctg
      241 gctcagcacc gtcacttcgg cgccgagcgc aaccgcgagc ttgacggcca gatgtccgag
      301 gccgccgagg ccgatgaccg cgaccttcgt gccgggtcct gcgttccagt gccgcagcgg
      361 tgagaaggtg gtgatgccag cgcacagcaa cggtgcggcg gcatccagcg aaatcgaatc
      421 cgggacgcgg agcacgtagt cctcgtcaac gacgatcgcg ccgctgtagc cgccctgtgt
      481 cggctgaccg tcgcgaccga cgccgttgta ggtgccgacc atccccgggt tgttgcagta
      541 ctgctcctcg cccgccaggc agtactcgca ttcgcggcag gagtcgacga aacagccgac
      601 gccgacgcgg tcgccgacct tgtacttggt cacctccgag ccgacctcgg tgacgatgcc
      661 tgcgatctcg tggccgggca cgatcgggta ggtcacgtcg ccccactcgg cccggacggt
      721 gtgaatgtcg gagtggcaga tgcccgcgaa gtggatgtcg aatgcgacat cgcggggccc
      781 gacgtcgcgg cgggtgatcg ttgtcttggt cagcggttcc gttgcggatg tggcggcgta
      841 ggcagatacg gcggtcgtca tgtgatccct cttcaatgca tgcgaaaaca agctcaacgg
      901 acaggtagaa gagccgcgac aatgcgactg ctcacttcaa ccccgctccg ccaagccata
      961 atccccggcg tcgctgtgag ttatctccta aagtcctggc gcgcggaggg cgccgttgaa
     1021 cgaggttcgc tcgtagttcg ccacaccggc cttcgtgtag gggtcgcgcg cgatcatgtc
     1081 gtcggcctgt tcgacggcga tgtcggcggt gatgagcatc ccgccggacc cgttgtcctg
     1141 gcggccggcg agaaggaggc ggcccgccgc gatctcctct ttcaggaact ggaggtgggc
     1201 cggacgggtc tgttcgacga cctcggccga ttgcagatac gtcgacctca agacgtgaat
     1261 cacccgacaa accctactcg gcgacggcat agcgttgaat ctcgagcctg ctccgttcgt
     1321 gcagagccga gttgtcgtta tcggtgatgc cgagcacccg catgcatgcg tctgccacgt
     1381 ggaacggcag gctggcgcgt tcgggacccg gtgccaccga ccacatgttg accagcgatt
     1441 cgaccagatg gaacggcagg tccgcggccg cctggttgac accggtctgt ttcacgacgg
     1501 agtggctgtg cgccagataa tgcagccgaa gccgctcgcg ctgagaccag aacggctcaa
     1561 ggcgagcgtc gcgtaactcg ggaagcaggt atagggcgcc gaggttccac cgtccgctga
     1621 gcagccggtc accgtcgaag gcggccagcg catgaaggta ctcggcggca gtcagcgcgg
     1681 ggtcgttgtc caacaggtcg ctgatgaacg acagcgtggg ggcgacggtc tggctgagca
     1741 gcgcgcacag aatgtcgtcc ttggtgttga agtagtgata cagcgacgcc tgccgaatgc
     1801 ccaccgactc cgcgatgctt cttgtggacg tgccggcgta ccccagggtt gtgaacaatt
     1861 cgccggcggc gtcgaggatt tcgtcacgcg cattcatccc cggacgccgc tgcgtgtgca
     1921 ggcgcggtcg cccggccctt gcaagcggca tggtcccatc gtggaccaca aacgagtcct
     1981 agcggcgaaa ccacaacttt ctgtcacatg acagaaactc gggacacgta tcggttacct
     2041 acgtcgccat tttggttacg ccaaggacac ccagcacgac tgcggcagcg gaaaactgtc
     2101 aggtgacagg caagcacgtt gttgtgccgc tatgccgaga ccttgatttc gacaccggga
     2161 gttgtccgtt gggcatcgcg atatccgaag ccgacgcgcg cacagcagtt gccggggtgc
     2221 cgccgacagc ggatcctgtg gcaaccgtcg gcgatctccg cgtgacgttc cggcgcaacg
     2281 gacgagatgt gcacgcgttg cgcggcgtct cgttgtcgat agcggcaggc gaaatcctgg
     2341 gactggtggg cgaatcgggt tccggcaaaa gcgtattggg attcagcgtc ctcgggcttc
     2401 ttcctgcggc cgcccacatc gaaggcgcgg tacgcgtctc cggcgcagac atggtgcgcg
     2461 gcggcgcgaa ggcgctgcgc aaggtgcgtg cgctcgatct cggcgcggtg tttcaggacc
     2521 cgatgtcctc gctcaacccg acgatgcgga taggcaagca ggtcgccgag gcggcgggct
     2581 ctgacgcgga agctttgcga ctgctcaccg ccgtcggcat cccggagccc aagcgcagga
     2641 tgcgggcgta cccgcacgaa ctatccggtg gcctgcggca gcgtgtcatg atcgccatcg
     2701 cagtcgcggg tgacccggaa ctcatcatcg ccgacgagcc cacgaccgcg ctggacgtaa
     2761 cggtgcaggc ccaagtgctt aggctgctgc ggcgactgcg tgacgaaatc ggctgcagca
     2821 tcctgttgat cacccacgac ctcggcgtgg cggcgcagat ctccgaccgc atcgcggttc
     2881 tgtatgccgg ccggatcgcc gagatcggtc ccacggcggc cgtgctgcaa acccccgccc
     2941 atccgtacac acacggactc ttgcgctcgc gcctcacgct cgacacccac cgagaccgcc
     3001 ggctggccgc cctggcaggg acggtcccca gcccggtgac gccgctgccg ggctgtgcgt
     3061 tcgcgccgcg gtgcgcgctt gccgccgagg actgctcgaa gacaccgccc gatccgatcg
     3121 ctgttggggc cgatcgggtg agcgcatgca tctttgggct cgacaaggtt gccggcgaga
     3181 tgggcacgaa gtcgaccaac accagccaac cgtttcccga acaggccgac cacgacgagg
     3241 agcccccttc ggtggtcatg cgcgatgtca ccaagacgtt ttcggtggcc aagcgctggc
     3301 tcagccgctc gacgggcaac cacggcaaac tgcaagcgct gcgcggcgtt tcgctgcgtg
     3361 tcggtcatgg cgaatcggtc gcgctggtcg gcgagagcgg ctcgggcaag tcgacgctgc
     3421 tgcggatcat cgcgggcctg gagaagccca cgtgggggtc ggttcagctg gcgggcgagc
     3481 agcgcccgca gatggtcttc caggatgcag gggcatcgct gacgccgtgg atgtcggtgg
     3541 gggagctcat cgccgaacgg ctccgcggga gcggggtggc gcgcgccgcg cgacgcgacg
     3601 ccgttgccga ggtgctcgag cttgtcggcc tgtctcccga tatcgcgaag tcacgggccg
     3661 gccagctgtc cggcgggcag cggcaacggg tgtcgttggc ccgcgcgacc gtcgtgccgc
     3721 cgtcggtgct gctgtgcgac gaacccacca gcgcgctcga tgtttcgctg gcggcgtcgg
     3781 tactcaacct catc
//
LOCUS       DASYLX010000189         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_886434,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000189 DASYLX010000000
VERSION     DASYLX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886434"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VGP27_04555"
     CDS             complement(<1..1482)
                     /locus_tag="VGP27_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEV7850624.1"
                     /translation="MILLGVGISIAVVVLVGFLVSKRIAGDSANFLVGGRSLPLMLVG
                     GALMGSAVDTNATLGNTDLAAEFGFWAGACLPLGLALCLFLTGVFVAKPMNRMGLTSF
                     PDYYRLRFGRQVEAVASLMLIVGFCLLVAGNLVAGGFLFNYFLGTPYWLGAVIIAILA
                     VAYTGTGGLIADAYTAIIQMSLVLIGAVGLLIWMAVTHGIAIAPGTGPLDLGQMTDSA
                     QGATINWATLIALGIGDIVAIDFMQRIFAAKSPETARRACFGAATGVVAICVPFGIVV
                     LSAKAFLPEQLDGPILFVLLDRYAPLALTIVVLCGLVGASMSTANGAILAISNVTVRN
                     LGGVRRVHVSGQRDPLLWATRIAMVPATVVAIVIAIYVKQTGILLTLAFDLLLACLVV
                     PFMLGLVWRKGTAVAALASIVVGLSVRLVLFAMTPTMYGLDNTILYIPNGVFDASFDG
                     WPTFIAFAASLITYVAIALVTPAGPIRGLDIAVAQDVDDALDEV"
     regulatory      complement(1508..1612)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     regulatory      1732..1798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            1809..>2710
                     /locus_tag="VGP27_04560"
     CDS             1809..>2710
                     /locus_tag="VGP27_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEV7850625.1"
                     /translation="MSDQPFEPLNREGAWAQQAEARLSDRREREEVERSLAFGLEAAP
                     TIKDRRISTFVRGELPHFAGERGTFLKCPFIEDVNQVDDAEVAFFGVPLDAGATYRPG
                     TRFGPQGIRRSTNLFGTYNYEMGVDLREQLNMVDIGDVFTIPGNLEKSFDQISQAMAH
                     VASKGVMPVVLGGDHSIGFPTIRGLAPYMDGNIGIIHFDRHVDTQESDLDERMHTTPW
                     FHATNIKNAPATNLVQIGIGGWQAPRAGVKVGRERNSTVITVGDVERVGVEKIAEMAL
                     EVAWKDAKAVYLSFDIDVIDAGFVP"
BASE COUNT          538 a          895 c          881 g          396 t
ORIGIN      
        1 cacctcgtcg agtgcgtcgt cgacatcctg cgccaccgcg atgtcgagtc cccggatcgg
       61 cccggcaggt gtcaccaaag cgattgccac ataggtgatc agagacgcgg cgaaggcgat
      121 gaaggtgggc cagccgtcga aggacgcgtc gaagaccccg ttgggaatgt agaggatcgt
      181 gttgtcgagg ccgtacatcg tcggcgtcat cgcgaacagc accagccgca cggacaggcc
      241 gacgacgatc gacgccagcg ccgcgactgc ggtgcccttg cgccacacca gcccgagcat
      301 gaacggcacc acgaggcagg ccagcagaag gtcgaaggcc agcgtgagca gaatgcccgt
      361 ctgcttcaca tagatcgcaa tcacgatcgc cacgaccgtc gccggaacca tcgcgatgcg
      421 cgtcgcccac agcagcggat cgcgttgtcc ggacacatgc acgcgccgga ccccgcccag
      481 gttgcgaacg gtcacgttcg agatggccaa gatggcaccg ttggcggtgc tcatcgacgc
      541 accgacaaga ccgcacagca cgacgatcgt cagcgccagc ggcgcgtatc ggtcgagcag
      601 cacgaaaagg attgggccgt cgagttgttc gggcaggaac gccttggccg agagcacgac
      661 gatgccgaac ggcacgcaga tcgccaccac accggtcgcg gcaccgaagc atgccctgcg
      721 ggcggtctcg ggcgatttcg ccgcgaagat ccgctgcatg aagtcgatcg cgacgatgtc
      781 gccgataccc agggcgatga gcgtcgccca gttgatggtg gcgccctgcg ccgagtcggt
      841 catctgaccc aggtccagcg ggcccgtgcc cggcgcgatc gcgatgccgt gcgtcaccgc
      901 catccagatc agcagaccga cggcaccgat gagaacgagc gacatctgga tgatcgccgt
      961 gtacgcatcg gcgatcagtc cgccggtgcc ggtgtaggcg acggcgagga tggcgatgat
     1021 gaccgcaccc agccaatacg gggtgccgag gaagtagttg aacaggaagc cgcccgcgac
     1081 caggttgccg gccaccaaaa ggcagaagcc gacaatgagc atcagcgatg ccaccgcctc
     1141 gacctggcgc ccgaatcgca gccggtagta atccgggaac gacgtcagcc ccatccggtt
     1201 catcggcttg gcgacgaaca cacccgtcag gaacaggcac aacgccaggc ccagcggcag
     1261 gcaggcgccg gcccagaagc cgaattcggc tgccaggtcg gtgtttccga gcgtggcgtt
     1321 ggtatcgacc gccgagccca tgagcgcgcc gccgaccaac atcagcggca ggctgcgacc
     1381 gccgaccaga aagtttgcgc tgtcacctgc tattcgtttc gagaccagga agccgacgag
     1441 tacgacgacc gcgatgctga tcccgacacc gagaagaatc atggggagcc tccttgcgag
     1501 ctcaggggcc tcccgggctt ttgtcccgcc gtggagcggc cggcttcgtc gtcggccacc
     1561 tgcgtctctc ggaccagacc cgcgaagcgg cggaacccta gacgcgcctc accagtgaac
     1621 gtgagtcgtc agtcagacaa accgcggttc gttacggcgg tgggcaagcc gtgttaacgg
     1681 ctcgttgcag tttcgactgg cgcgtcgacg gatcgctgcc aaaataagga accaggagtc
     1741 gccgaccggc tccccggacg aggtgcgtcg tacccggctt gcgacaagac ggcgctcgag
     1801 gagtcatcat gtccgatcaa ccgtttgaac ctctcaatcg cgaaggcgca tgggcgcagc
     1861 aggccgaggc ccggctgtcc gaccgcaggg agcgcgagga ggtcgagcgc tcgctcgcgt
     1921 tcggtctcga ggcggcgccg acgatcaaag accgccggat ctccacgttc gtgcgcggcg
     1981 aactgccgca tttcgccggc gaacgcggaa cgttcctgaa gtgcccgttc atcgaggacg
     2041 tcaaccaggt cgacgacgcc gaggtggcgt tcttcggcgt cccgctcgac gcgggtgcca
     2101 cctaccggcc cggaacccgt ttcggtccgc agggaatcag gcgctccacc aacctgttcg
     2161 gcacctacaa ctacgaaatg ggcgtcgatc tgcgcgaaca gctcaacatg gtcgacatcg
     2221 gcgacgtgtt cacgattccc ggcaatctgg agaagtcgtt cgaccagatc agccaggcga
     2281 tggcgcatgt cgcgagtaag ggcgtgatgc ccgtcgtgct cggcggggac cactccatcg
     2341 gcttccccac catccgtggt ctggcgccct acatggacgg caacatcggg atcatccact
     2401 tcgaccggca cgtcgacacg caggagtccg atctcgacga gcggatgcac accacgccgt
     2461 ggttccacgc caccaacatc aagaacgcgc ccgcgaccaa cctggtgcag atcggcatcg
     2521 gcggctggca ggcgccgcgg gcgggcgtaa aggtgggtcg cgagcgcaac tcgacggtca
     2581 tcaccgtcgg cgacgtcgag cgcgtcggcg tcgagaagat cgccgagatg gcgctcgagg
     2641 tcgcgtggaa ggacgccaaa gccgtctacc tgtccttcga catcgacgtc atcgacgccg
     2701 ggttcgtgcc
//
LOCUS       DASYLX010000190         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3739161,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000190 DASYLX010000000
VERSION     DASYLX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3739161"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGP27_04565"
     CDS             <1..495
                     /locus_tag="VGP27_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEV7850626.1"
                     /translation="TVAAYAAPKSSALWREVASELADGLRKDATEVRIEDGPWGREVV
                     GAAPAGVVRFIGVDGYRWMIRCVVNGPEQTIDELSDQARQALADTVVRRGETPLPVRT
                     PLPVQLPEPMAAQLQAAAAEQAAAQQAAQQQQQPPQTPQNEPVARRSEHGSAMQQLRT
                     ITGG"
     gene            complement(502..1200)
                     /locus_tag="VGP27_04570"
     CDS             complement(502..1200)
                     /locus_tag="VGP27_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850627.1"
                     /translation="MAVDLRGVTTVLLPGTGSDDDYVYRAFAAALHEAGATVVAPPPQ
                     PDRLIEGYICELDNAARLGAIAVGGVSIGAAVAANWALAHPTRAIAVLAALPPWTGSP
                     ETAPAALAARHSADVLRRDGLVSATSQMRASSPAWLADELTRSWLGQWPSLPDAMEAA
                     SRYASPTCAELERLTVPMGVAAATDDPVHPVEVGLEWVAAAPRAALHTVTLEQMGIDP
                     SVLGTACVAALLDA"
     gene            1331..1702
                     /locus_tag="VGP27_04575"
     CDS             1331..1702
                     /locus_tag="VGP27_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV7850628.1"
                     /translation="MATAEGYLRRLTRRLTDDPEQLDAEELTEEATNTGAQKAVDCQR
                     GQEVTMVGTLRSVEANGKGCAGGIKAELFDGTEAVILVWLGQRRIPGIESGRTLRVHG
                     RMGKLENGTKAIYNPHYEIQK"
     gene            1771..>2130
                     /locus_tag="VGP27_04580"
     CDS             1771..>2130
                     /locus_tag="VGP27_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEV7850629.1"
                     /translation="MGGISGLIYSSLPVLVFVPVSSMFGLMPAIIAALVIAALILVWR
                     LARKESAQPAISGFIGVGFCALIAYVMGESKGYFLYGIWMSLFWAVVFLVSVLIRRPL
                     VGYIWSWVNGHDRGWRNV"
BASE COUNT          349 a          709 c          741 g          331 t
ORIGIN      
        1 accgtggcgg cttatgccgc gccgaagagt tcggcgctgt ggcgggaggt cgcatcggag
       61 ctggcggacg ggctgcgcaa ggacgccacc gaggtgcgga tcgaggacgg cccgtggggc
      121 agggaagtcg ttggcgcagc acctgccggc gtggtccggt tcatcggtgt cgacggctac
      181 cggtggatga tccgctgcgt cgtcaacggg ccggagcaga ccattgacga actgagtgac
      241 caggcgcgac aagccctggc cgacaccgtt gtccgtcgcg gtgagacacc gctgccggtg
      301 cgcaccccgc ttccggtgca gctgcccgag ccgatggccg cacagctgca ggccgccgcc
      361 gcggagcagg ctgctgccca gcaggccgcg cagcagcaac agcagccacc gcagacgcct
      421 cagaacgagc ctgtcgcacg ccgcagtgag cacgggtcgg cgatgcagca gctgcgcacc
      481 atcaccggcg gctagaaggg ctcacgcgtc gagtagggcg gccacgcacg ccgtgccgag
      541 caccgacggg tcaatgccca tttgctcgag cgtgacggtg tgcagcgccg ctctcggcgc
      601 ggccgccacc cactccagcc cgacctccac cggatgcaca gggtcgtcgg tggccgccgc
      661 gacgcccatc ggcaccgtca gccgctccaa ctccgcgcag gtaggcgagg cgtaccggga
      721 cgccgcctcc atcgcgtcgg gtagcgacgg ccactgcccg agccaggacc gggtcagctc
      781 gtcggccagc caggcggggc tcgacgctcg catctgtgac gtcgccgaca ccaacccgtc
      841 cctgcgaagg acatccgccg aatgccgggc ggccagcgcg gcgggcgcgg tctcgggcga
      901 gcccgtccaa ggcggcagcg ccgccagcac cgcgatcgcc cgggtcggat gggccaacgc
      961 ccaattggcc gccaccgcgg ctccgatcga caccccaccg acggcgatgg cgcccagccg
     1021 tgccgcgttg tcgagttcgc agatgtagcc ctcgataagc cggtcgggtt gcggcggtgg
     1081 tgccacgacg gtggcgcctg cttcgtgcag agcggctgcg aacgcgcggt agacgtagtc
     1141 gtcgtcggat ccggtgcccg gcagcagaac cgtcgtcacg ccgcgcagat cgacagccat
     1201 tatgagatcg tgccgtgtgg tttaaatcac cggcgtggcg ggtcaccctc agcgtggcaa
     1261 tactgaccca acaggtctac cgtggcgttg gttgggatcc acggaggatt cacagattgg
     1321 cagggaggcc atggctacgg ccgaagggta tctgcggcgg ctcactcgtc ggctcacgga
     1381 cgatcccgag caactcgatg cggaggagtt gaccgaagag gccaccaaca ccggcgccca
     1441 gaaggccgtc gactgtcagc gcggccagga agtcacgatg gtcggtaccc tgcgcagcgt
     1501 cgaagccaac ggcaaggggt gtgcgggcgg catcaaggcc gaactgttcg acggcaccga
     1561 ggcggtcatc ctggtgtggc tgggccagcg ccggataccc ggcatcgagt cgggccgcac
     1621 gctgcgggtg cacggacgaa tgggcaagct cgagaacggc accaaggcga tctacaaccc
     1681 gcactacgaa attcagaagt aggccgcgcg ggcacgtacc gttgactcgt gtccgataag
     1741 caatccggcg ctcaaattct gcttgcccag atgggcggca tcagtggcct gatctattcg
     1801 tcgctgcccg ttctggtgtt cgtcccggtc tcctcgatgt tcggcctgat gcccgctatc
     1861 atcgccgcgc tggtcatcgc cgcgctgatc ctggtgtggc ggctggcgcg caaggaatcc
     1921 gcgcagccgg ccatctccgg tttcatcggg gtcgggttct gcgcgctgat cgcgtacgtg
     1981 atgggcgaat ccaagggcta tttcctgtac ggcatctgga tgtcgctctt ctgggccgtg
     2041 gtgttcctcg tctcggtgct gatccgcaga cccctcgtcg gctacatctg gagttgggtc
     2101 aacggccacg atcgcggttg gcgaaatgtg
//
LOCUS       DASYLX010000191         5109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_227946,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000191 DASYLX010000000
VERSION     DASYLX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227946"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VGP27_04585"
     CDS             complement(<1..335)
                     /locus_tag="VGP27_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HEV7850630.1"
                     /translation="MAEDLLEAYRTGDPVAPLTDRSPDLTIADGYAIQQAQVAKWQAE
                     GRVTKGRKVGLTSAAVQRQMGVAQPDFGVLHDQMFYAESEVVPISAFLAPRIEPETAL
                     VLGRRLAGPG"
     gene            complement(359..1294)
                     /locus_tag="VGP27_04590"
     CDS             complement(359..1294)
                     /locus_tag="VGP27_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7850631.1"
                     /translation="MIELQDIAYVRSGVTDLERATRFAVDIVGLDPVVLDPGVSYLRA
                     DHRHHCLALVESDVAGVHASAFSLRDDAALEAAESELRRYGCTVWRGSEEQSRQRRVR
                     NFIGFDDPFGNHVELVTGQSTLARPINFGRPAAGIAEFGHLCLDAPDVRAAHHFWSTV
                     FSGRVSDWIGDGACLMRIDPVHHKLAFFQNDAGPGFCHINFQVETLDDVMRNWRFLQQ
                     QGVHILHGPGRHPTSTAIFVYFLGPDNLTYEYSFGVQLIQDDAAWTPRMFELQEPGSI
                     DMWLGPTQRTVSQSQLAGGAPVTAATSPAEPVVGL"
     gene            1667..3160
                     /locus_tag="VGP27_04595"
     CDS             1667..3160
                     /locus_tag="VGP27_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7850632.1"
                     /translation="MQTSLEGNKRDVAVLLDREWLMLIGGDRVAASDGATMQITAPHD
                     GSVIGSVPAAGPADIDAAVSAAAAAFPAWHGTTLLERAEMIRAFAARLRARATEFGLL
                     DAIDTGNPVTAMTGDVMMAARWLDYHASVAFGITGDTLPSMTGSWLMTRKEPYGVVGR
                     IIPYNHPILFAAAKVGAPLITGNTLVLKVPDQAPLSSLLMAEVVGASFPPGVVNIVSG
                     SGAVAGDALVRHSDVKRIALIGSVPTGQKVMAAAAEVGIKHVTLELGGKNAMIVCPDA
                     DPAAVVEGAAFGMNCHWSQGQSCGSTTRLFLHESLHDQVVAGLVERLKSIKIGHPLDP
                     ATEMGCLVSSAQYDKVHHYIDSARREGARLVTGGGRPHGDEFQAGFYVEPTVFTDVDM
                     SMTIAREEIFGPVLSVLRFTDLDAAVAQANALPFGLTGAVWSNDIATALSVADRLDTG
                     YVWINGSGSHFLGAPFGGHKNSGTGSEEGIEELESFLQTKTVNIPLR"
     gene            3260..3841
                     /locus_tag="VGP27_04600"
     CDS             3260..3841
                     /locus_tag="VGP27_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7850633.1"
                     /translation="MPRRPRNPPSIAEQIALAADREGPDFDTAVLGLTLALFRTATAF
                     ERAHVTELLPHGLSIIQLNILTVLDRATEPLTMGALGQAVSVLPANLTGVVDGLVKRG
                     YVERIPNPSDRRSFLIRIGKVGRSFLRKFLPGHWAHLHNLMSDLTPAQRRQLQSLLGK
                     FLESIEQNGKDTADGVSSDRVRRPARRGGAVPT"
     gene            3838..4863
                     /locus_tag="VGP27_04605"
     CDS             3838..4863
                     /locus_tag="VGP27_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850634.1"
                     /translation="MTRVGSGVIDLHTHGIPRRLPAFGRRFAGSWPELMTTGPCTADI
                     MLGERHFRSITDQCWNLERRITDMDADGVARQVISPIPITFAYGLDTDGVAELSRVQN
                     EWIAEAVRALPARFSGLGTVPLQDPDRAAEMVVEIRRDLGLAGVEIGSNVRDVNLDDR
                     ALDTFFAACAEQRALVFIHPWQVLGANRLTHYGLAESIGMGAETATAAATLVMGGVLD
                     RHPDLDVLLAHGGGAFLALLPRIERFWANLPGAPSCVDRPSSYARRFFYDSLVFDPEV
                     VAALIGRVGADRVAVGTDYPFAIAERPAGAALLRAGLSEATTEAVSTHTAERLLGLTR
                     AGAEVPG"
     gene            complement(4860..>5109)
                     /locus_tag="VGP27_04610"
     CDS             complement(4860..>5109)
                     /locus_tag="VGP27_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEV7850635.1"
                     /translation="RARTPVQRELLDTAVCTSSLIWESSYAEAEQAMLGDPFRCGVAE
                     NAASLEALLTYAHEQGFSRRQLAPEDVFVPSTVSAARV"
BASE COUNT          877 a         1696 c         1648 g          888 t
ORIGIN      
        1 ccgggaccgg cgaggcgacg ccccagtacc agggcggtct ccggctcgat ccggggcgca
       61 agaaacgccg agatgggtac gacctccgac tccgcataga acatctggtc gtgcaacacc
      121 ccgaaatccg gctgcgcgac ccccatttgc cgttgcacgg ccgccgaggt gaggccgacc
      181 ttgcggccct tggtcacccg accctctgcc tgccacttgg cgacctgagc ctgttgaatc
      241 gcgtagccgt cggcgatggt caagtcagga gaccgatccg tcagcggcgc aacgggatct
      301 cccgttcgat aggcctccag caggtcctcg gccatccgat cgcactcatc ggcacgcatc
      361 agagaccgac gacgggttcg gccgggctcg tcgccgccgt caccggcgcg ccgcctgcca
      421 gttgcgactg ggagaccgtc cgctgggtgg gacccagcca catgtcgatg gagcctggtt
      481 cctggagttc gaacatgcgc ggcgtccatg ccgcgtcgtc ctggatcaac tggacgccga
      541 acgagtactc gtaggtcaga ttgtccggcc cgaggaagta gacgaagatg gccgtcgacg
      601 tgggatggcg accggggccg tgcaggatgt gcacgccttg ctgttgcagg aatcgccagt
      661 tgcgcatcac gtcgtccagc gtctcgacct gaaagttgat gtggcagaag ccgggtcccg
      721 cgtcattctg gaagaacgcc agtttgtgat ggacgggatc gatgcgcatc aggcatgcgc
      781 cgtcaccgat ccagtcggac acccgcccgc tgaacacggt cgaccagaag tggtgtgcgg
      841 cgcgcacatc gggagcgtcg aggcagaggt gaccgaattc cgcgatgccg gcagcgggcc
      901 gtccgaagtt gatcggccgt gccagcgtgc tctggccggt gaccagttcc acgtggttgc
      961 cgaacgggtc gtcgaaaccg atgaagttac gcacacggcg ttgtcgggat tgttcctcgg
     1021 aaccacgcca caccgtgcag ccataccgcc gcagctccga ttcggcggcc tccagggcgg
     1081 catcgtcgcg caacgagaac gccgaggcgt ggaccccggc cacgtcactc tccaccagcg
     1141 ccaggcagtg gtggcgatga tcggcgcgca ggtaggacac gccggggtcc agcaccaccg
     1201 gatccaatcc cacgatgtcg acggcgaacc gtgtcgcgcg ttccagatca gtcaccccgg
     1261 accgcacata ggcgatgtcc tgcagttcga tcatcccgga cctctctggt gaaaccgaca
     1321 cgaatcagtc agcacccgag gcgcgccgga gatccgtcgt ccgcagatac ttacttatga
     1381 ccctgaacta tattgtgttc gagccgccgt ggtcaaaggt ttcgccaccc ttctttcggc
     1441 ggtgcggcgt ctggcacggt agttgacttg atgggtagtc gaatcattgt tgtgcacaag
     1501 cgagttacac cctcgcgggt gatggaaacc gccgggccga gataacggcc gcaggcccgt
     1561 tagttgtatc agtgaacctt ttttgaacat gacttgtgaa cgcggaatgc acgctagagt
     1621 ggaagcacgc cggctgtgta ctactcgagg aagtgagcat cggcacgtgc aaacctcgtt
     1681 ggagggcaac aagagggacg tcgcagtcct gctcgaccgt gaatggctca tgctcatcgg
     1741 tggcgatcgc gtcgccgcca gcgatggcgc gacgatgcag atcactgcgc cacacgatgg
     1801 ctccgtaatc gggagcgtgc cggccgccgg gcctgccgac atcgatgcgg ccgtgagtgc
     1861 cgccgccgcg gctttcccgg cgtggcacgg cacgacgctg ctcgaacgcg ccgagatgat
     1921 ccgggcgttc gctgcgagat tgcgggcccg tgcaacggag ttcggtctgc tcgacgccat
     1981 cgacaccggt aatcccgtca ccgcgatgac cggcgacgtg atgatggcgg cccggtggct
     2041 cgactatcac gcctcggtgg ccttcggcat caccggcgac accctcccgt cgatgaccgg
     2101 cagctggctc atgacgcgca aggaaccgta cggcgtggtc gggcggatca ttccctacaa
     2161 ccatcccatc ctgttcgccg ccgccaaggt cggcgctccg ctgatcacgg gcaacaccct
     2221 ggtcctcaag gtgcccgatc aagctccgct gtcctctctg ctgatggccg aggtggtggg
     2281 agcatcgttc cctcccgggg tcgtcaacat cgtttccggc agcggcgccg tcgcaggcga
     2341 cgccctcgtc cgtcactccg acgtcaagcg catcgcgctc atcgggagcg tcccgacggg
     2401 ccagaaggtg atggccgcgg cagccgaggt cggcatcaag cacgtcaccc tcgagctcgg
     2461 cggcaagaac gcgatgatcg tctgcccgga cgccgatccg gccgccgtcg tcgaaggtgc
     2521 ggcgttcggc atgaactgcc attggagcca gggccagtcg tgcggctcga cgacgcggct
     2581 cttcctgcac gagtcgcttc acgatcaggt tgtcgccggg ctggtggagc gactgaagtc
     2641 gatcaagatc ggtcatccgt tggaccccgc gacggagatg ggctgcctgg tgtcctcggc
     2701 ccagtacgac aaggtgcatc actacatcga ctcggcaaga cgcgagggcg cccgactggt
     2761 caccggcggc ggacgccccc acggcgacga gttccaggcc gggttctatg tcgagccgac
     2821 ggttttcacc gatgtcgaca tgtcgatgac gatcgccagg gaagagatct tcggtccggt
     2881 cctatcggtt ctgcgattca ccgatctcga cgctgccgta gcgcaggcca acgcgttgcc
     2941 gttcggccta accggggcgg tctggagcaa cgacatcgcg accgcccttt cggtggccga
     3001 ccgactcgac accggctacg tgtggatcaa cggaagtggc agccatttcc tcggcgctcc
     3061 attcggcggc cacaagaaca gcggcaccgg ttccgaggag gggatcgagg agcttgagag
     3121 cttcctgcag accaagacgg tcaacattcc gctccgctga atcttgggtc agcggcggag
     3181 cacggaccgg atagttcggt ttggataaag tacagattca gaactacccg acgtagtgca
     3241 ccgaccggga ggccacctca tgccgcgacg gccccgcaat ccaccctcga tcgccgagca
     3301 gatcgccctt gccgccgacc gcgagggtcc cgacttcgac acggcagttc tcgggctcac
     3361 tcttgcgctc ttccgcacgg cgaccgcgtt cgagcgtgct cacgtcaccg aactgctgcc
     3421 ccacggcctc agcatcatcc agctcaacat cttgacggtc ctggaccgcg ccactgagcc
     3481 actgacgatg ggcgctctcg gacaagcggt atcagtgcta ccggcgaacc tcaccggcgt
     3541 cgtcgacggt ctggtgaaac gaggctatgt cgagcggatc cccaatccca gcgatcgccg
     3601 atcgttcctg atccgcatcg gcaaggttgg ccgcagcttc cttcgcaagt tcctgccggg
     3661 ccactgggca cacctacata acctgatgag cgatctgacg ccggcccaga ggcgtcagtt
     3721 gcagtcgctg ctcggcaagt tcctcgagtc gatcgagcag aacggcaagg acaccgcaga
     3781 cggggtcagt tccgaccgcg tccgacgtcc ggcacgtcgc ggcggcgcgg tgccgacgtg
     3841 acgcgggtgg ggtccggcgt catcgacctc catacccacg ggataccccg gcgcttgcct
     3901 gcgttcggca ggcgctttgc gggatcctgg ccggagttga tgacgacggg accctgcacg
     3961 gccgacatca tgctgggcga gcgtcatttc cgatccatca ccgaccagtg ctggaacctc
     4021 gaacgtcgca tcaccgacat ggatgctgac ggcgttgccc ggcaggtgat ttcgcccatt
     4081 cccataacct tcgcctatgg tctggacacc gacggtgtcg ccgaactctc gcgcgtgcag
     4141 aacgaatgga tcgccgaagc ggtccgggca ctcccggctc ggttcagcgg actgggcacg
     4201 gtgccgctgc aggatcccga ccgcgcggcg gagatggttg tcgagatccg acgggacctt
     4261 ggcctggccg gggttgagat cgggtccaac gtgcgcgacg tgaacctcga cgaccgggct
     4321 ctggacacct tcttcgcggc ctgcgccgag caacgcgcgc tcgtgttcat ccacccgtgg
     4381 caagtgctgg gcgccaaccg tctcacccac tatggactcg ccgagtcgat cgggatgggc
     4441 gccgaaaccg caacggccgc tgcaacgttg gtgatgggtg gcgttctcga ccgtcatccc
     4501 gatctggatg tgctgcttgc ccacggtggt ggggccttct tggcgctgct cccccggatc
     4561 gaacggttct gggcgaatct tcccggggcg ccgagttgcg ttgaccgacc gtcgagctat
     4621 gcgcgccgat tcttctacga ttcactcgtc ttcgacccgg aagtggtcgc ggcgctgatc
     4681 ggacgcgtcg gggccgaccg cgtggccgtc gggaccgact accccttcgc gatcgccgaa
     4741 cgccccgccg gggcggctct gctgcgggcc ggcctgtccg aggccaccac cgaggcggtc
     4801 agcacacaca ccgccgagcg gctgctgggg ctcacccgcg ccggggcgga agttcctggc
     4861 taaacccttg cggcactgac ggtcgacggc acgaacacgt cttcgggtgc cagctgtcgt
     4921 cggctgaacc cctgctcgtg ggcgtaggtc agcaacgcct ccaggctggc ggcgttctcg
     4981 gcgacaccgc agcggaacgg atcaccgagc atcgcctgtt ccgcctccgc gtacgaggac
     5041 tcccagatca ggctgctggt acagacggcg gtatcgagaa gctccctctg tacgggcgtg
     5101 cgtgcccgt
//
LOCUS       DASYLX010000192         6915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2312912,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000192 DASYLX010000000
VERSION     DASYLX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312912"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGP27_04615"
     CDS             <1..693
                     /locus_tag="VGP27_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEV7850636.1"
                     /translation="EYTTAPADFCAYVPEGVSPDVAAASLLKGMTAHYLLKSVYPVQQ
                     GDSVLVHAGAGGVGLILTQWATSQAVRVISTVSTPEKAELSRQAGAAEVLDYPEDPDE
                     FGARIKELTDGGVAAVYDGVGAATFDASLASLAVRGTLALFGASSGPVPAFDPQRLNA
                     AGSVFLTRPTLAHYTRTADEFSWRAGELLDAIASGTISVTVSERYRLEEAERAHRDLQ
                     GRKTVGSIVLVP"
     gene            complement(690..1631)
                     /locus_tag="VGP27_04620"
     CDS             complement(690..1631)
                     /locus_tag="VGP27_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEV7850637.1"
                     /translation="MVSPDNFVRAETDLYFGNIVKDGGFGKLKHNRELSPLDNQLVVR
                     QNRDTLYSAAVFDLDAGPVTVTLPDPGTRFMSMQVIDQDEYTHDVIYSKGLHTFTRNQ
                     VGTRYVALAVRILVDPGDPADIADVHALQDAFVVKKDKTGSFEVPKWDPLSQKAVRDA
                     LIALSNTLPDTRGTFGTKGDTDPVRHLIGAANAWGGNPDRDALYLTVVPPRNDGTTVH
                     RLTVGAVPVDGFWSVTVYNKDGYFSPNPHNAYSFNNVTAKKEADGNTTIQFGGCDDDK
                     APNCLPITAGWNYMVRLYRPQQQLLDGSWTFPQAQAL"
     gene            complement(1818..2780)
                     /locus_tag="VGP27_04625"
     CDS             complement(1818..2780)
                     /locus_tag="VGP27_04625"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV7850638.1"
                     /translation="MSIRDGHLLDGAPEHTSSAARLRRRIRVMLLGYLSLTKPRVIEL
                     LLVTTIPAMLLADRGAVNPLLILNTLVGGLLAAAGANTLNCVADADIDKKMKRTERRP
                     LARDSVARSHALIFGLALSVASFFWLWWTTNMLSAHLAGATIAFYVLVYTLLLKRRTS
                     QNVVWGGAAGCMPVMIGWSAVTGTIGWPALVMFAVIFFWTPPHTWALAMRYKEDYRAA
                     GVPMLPAVATERQVTKQILVYTWLTVVATLTLALATGWLYASVALVAGAWFLAMAHQL
                     NAGVRRGEPVKPLRLFLQSNNYLAVVFCALAVDSALDLPTLLGR"
     gene            2977..5067
                     /gene="tkt"
                     /locus_tag="VGP27_04630"
     CDS             2977..5067
                     /gene="tkt"
                     /locus_tag="VGP27_04630"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV7850639.1"
                     /translation="MTTVEEISALTRPNHPDDWTDLDSEAVDTVRVLAADAVQKVGNG
                     HPGTAMSLAPLAYSLFQRQMRHDPSDVHWLGRDRFVLSAGHSSLTLYIQLYLGGFGLE
                     LSDIESLRTWKSKTPGHPEFRHTKGVEITTGPLGQGLASAVGMAMASRYERGLFDPDA
                     APGESPFDHYIYVIASDGDMEEGVSSEASSLAGTQQLGNLIVFYDHNQISIEHDTNIA
                     LSEDVPARYRAYKWHVQEVEGGENVTGIEEAIEAAKAVTDKPSFISVRTIIGYPAPTK
                     MNTGAAHGSALGDEEVAATKRILGFDPDKTFEVRDEVIEHTRKLVARGKEAHEKWQPQ
                     FDAWAEREPERKKLLDRLLAQELPDGWDADITHWEPGSKALATRAAFGQVLNDVAPKL
                     PELWGGSADLAGSNNTTIKDVKSFGPPSISTEDFSADWYGRVLHFGIREHAMGSILSG
                     IVLHGPTRAFGGTFLQFSDYMRPAVRLASLMDIDTIYIWTHDSIGLGEDGPTHQPIEH
                     LAALRAIPNLSVVRPGDPNETAYAWRSILARGNGSGPVGFVLTRQGIPVLEGTDSEGV
                     AKGGYVLGGIPDELPDVVLIGTGSELQLAVEAQKLLAEKDITATVVSMPCVEWFESQP
                     AEYRDKVLPPAVSARVAVEAAVAQSWYKLVGDTGEIVSIEHFGESADDKTLFREFGFT
                     PEAVVAAAERSLDN"
     gene            5087..6202
                     /gene="tal"
                     /locus_tag="VGP27_04635"
     CDS             5087..6202
                     /gene="tal"
                     /locus_tag="VGP27_04635"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV7850640.1"
                     /translation="MAQNPNLAALSAAGVSVWLDDLSRDRLRTGNLQELIDTKCVVGV
                     TTNPSIFQAALSKGHAYDDQIRELAERGADVDATIRTITTDDVRDACDVLRPQWEASD
                     GVDGRVSIEVDPRLAHDTDKTVQQAIELWKIVDRPNLFIKIPAMMEGLPAITSVLAEG
                     ISVNVTLIFSVERHREVMDAYLAGLEAAKQGGYDLSKIHSVASFFVSRVDAEIDKRLE
                     KIGSDDALALRGQAGVANARLAYAAYQEVFVGGDRFEALKGDGARVQRPLWASTGVKN
                     PEYSDTLYVTELVAPNTVNTMPEKTLEAVADHGVIRGDTVTGTAGAAQEVFDKLEAVG
                     IDLRDVFLTLENEGVEKFEKSWQELLEETRTQLDDKK"
     gene            6199..>6915
                     /locus_tag="VGP27_04640"
     CDS             6199..>6915
                     /locus_tag="VGP27_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306775.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV7850641.1"
                     /translation="MSAEGKSVETSAAPADWRNPLRDKRDKRMPRIPGPCSVVIFGVT
                     GDLARKKLIPAIYDLANRGLLPANFGLVGFARRDWADGDFSKLVCDSVQQHARTPYRQ
                     EVWDRLAEGIRFVQGTFDDEKAFQRLAETLEKLDAERGTEGNHAFYLSIPPNAFQMVC
                     EQLQNSGLARPQPGRWSRVVIEKPFGHDLASAKELNNTVNSVFPEESVFRIDHYLGKE
                     TVQNILALRFANELYEPIWNN"
BASE COUNT         1254 a         2258 c         2284 g         1119 t
ORIGIN      
        1 gaatacacca ctgccccagc cgatttctgc gcctatgtgc ccgagggagt gagcccggac
       61 gtggcggcgg catcgctgct caagggtatg acggcgcact acctgctcaa gtcggtctac
      121 ccggtgcagc agggcgacag cgtcctcgtg cacgcaggcg cgggcggggt cggcctcatc
      181 ctcacccagt gggccaccag ccaggcggtc cgggtgatca gcaccgtgtc gacgccggag
      241 aaggccgagt tgagtcgcca ggcgggcgcc gccgaggtgc tcgactatcc agaggacccc
      301 gacgagttcg gcgccagaat caaggagctg accgacggcg gggtcgcggc ggtctacgac
      361 ggcgtcggcg ccgccacctt cgacgccagc ctcgccagcc tggccgtcag aggaacgctg
      421 gccctgttcg gtgcgtccag tgggcccgtc cctgcgttcg acccgcagcg actcaacgca
      481 gcaggctcag tgttcctgac ccggccgaca ctggctcact acacccgcac cgccgatgaa
      541 ttctcttggc gggcaggcga attgctcgat gcaatcgcgt cgggcaccat cagtgtgacg
      601 gtcagtgaac gctaccgcct cgaggaagcc gagcgtgcgc accgcgacct gcaggggcgc
      661 aagaccgtcg gctccatcgt gctggtgccc tagagagcct gggcctgcgg gaaggtccat
      721 gagccgtcga ggagctgctg ctgcggccgg tacagccgga ccatgtagtt ccagcccgct
      781 gtgatcggca ggcagttcgg cgccttgtcg tcgtcgcacc cgccgaactg tatcgtggtg
      841 ttcccgtcgg cctctttctt cgcagtgacg ttgttgaacg aataggcgtt atgcgggttg
      901 ggtgagaagt agccgtcctt gttgtagacg gtgaccgacc agaatccgtc gacaggtacc
      961 gcgccgacgg tcaaccggtg caccgtggtg ccgtcattgc gcggcggcac gacggtgaga
     1021 tagagggcat ccctgtccgg gttgccgccc caggcattgg ccgcgccgat caggtgccgt
     1081 accgggtcgg tgtctccctt ggtgccgaac gtgccccggg tgtcgggcag ggtgttcgac
     1141 agcgcgatga gcgcgtcgcg aaccgccttc tggctcaacg gatcccactt cggcacctcg
     1201 aagctgccgg tcttgtcctt cttgacgacg aacgcgtcct gcagggcgtg gacgtcggcg
     1261 atgtcggcgg ggtcaccggg gtcgaccagg atgcgcaccg cgagcgcgac gtagcgcgtt
     1321 ccgacctggt tgcgggtgaa ggtgtgcaga cccttgctgt agatgacgtc gtgggtgtac
     1381 tcgtcctggt cgatcacctg catcgacatg aatctcgtgc cggggtcggg cagtgtcacg
     1441 gtcaccggcc cggcgtcgag atcgaacacg gccgcggagt aaagggtgtc cctgttctgc
     1501 cgcaccacca gctggttatc caacgggctg agttcgcgat tgtgtttcag ctttccgaag
     1561 ccgccgtcct tgacgatgtt gccgaagtag aggtcggtct cggctcggac gaagttgtcg
     1621 gggctgacca tgaccagacc gtcgggcgtg gtgcggggcg gttccttcat cacgtcgcct
     1681 gactcctcgg ttgcgggtgg caccgacgat ccgcagccgg caagaacgac ggctgcggtg
     1741 aggacggcga gccggacgaa ggcgggcatg gcccggacgg tactgcggcg tccggcccta
     1801 ttgacgaaaa ccgtgaacta gcggccgagc agcgtcggca ggtccagcgc cgagtcgacg
     1861 gcgagcgcgc agaacacgac ggccagataa ttgttggact gcaggaacag tcgaagcggc
     1921 ttgaccggct cgccgcggcg caccccggca ttcagctggt gggccatcgc caggaaccag
     1981 gcaccggcga ccagcgccac cgacgcatac aaccagccgg tcgccagcgc caacgtcagc
     2041 gtcgccacga cggtcagcca cgtgtagacg aggatctgct tggtcacctg tcgctcggtc
     2101 gccaccgcgg gcagcatcgg cacgcctgcc gctcggtagt cctctttgta gcgcatggcc
     2161 agcgcccagg tgtgcggtgg cgtccagaag aagatgaccg cgaacatcac cagggcgggc
     2221 cagccgatcg tgcccgtcac cgccgaccag ccgatcatca cgggcatgca gcctgccgcc
     2281 ccgccccaca cgacgttctg ggacgtccgc cgcttgagca gcagtgtgta gacgaggacg
     2341 tagaacgcga tcgtcgcgcc cgcaagatgt gcggacagca tgttcgtcgt ccaccagagc
     2401 cagaagaacg acgcgacgga cagcgccagc ccgaagatca gcgcgtggct gcgcgcgacg
     2461 ctgtcccgcg ccagcggtcg ccgctcggtc cgcttcatct tcttgtcgat gtcggcgtcg
     2521 gcgacgcagt tcaacgtgtt tgcgcccgcc gcggccagca ggccacccac cagggtgttc
     2581 agtatcagca gcggattgac ggctccgcgg tcggccaaca acatcgcggg aatggttgtg
     2641 accagcagaa gctcgatcac ccggggcttg gtcagcgaca agtacccgag aagcatcacg
     2701 cggattctgc gccggagccg cgcggccgac gacgtatgct ccggcgcccc gtcaagcagg
     2761 tggccgtcgc ggatgctcac ggtagaaaac tcctcaaagg gcgcgcagcg cgcctcgtct
     2821 actacagccg atggtagacc ggagggcgcc tcggaaccca gctgggtcga ttcggtaaat
     2881 gaacagctaa tgatgcgcga cgcagcctgc aacgattccc tgcgccgcac tagggtatga
     2941 ggtagttcag gcctgccgat ctggaggagt caactggtga caactgtcga agagatttcc
     3001 gcccttaccc gccccaacca tccggacgac tggaccgacc tggactcgga agccgtcgat
     3061 accgtccgcg tactggcggc ggatgcggtg cagaaggtcg gcaacggtca ccccgggacg
     3121 gcgatgagcc tcgctcccct ggcctactca ctgtttcagc gccagatgcg ccacgatccc
     3181 agcgatgtgc actggctggg acgtgaccgg ttcgtgctgt cggccgggca ctccagcctg
     3241 accctgtaca tccagctgta cctgggcggc ttcggcctcg agctgtctga catcgagtcg
     3301 ctccggacgt ggaagagcaa gacacccggc catcccgagt tccggcacac caagggcgtg
     3361 gagatcacca ccggcccgtt gggccagggt ctggcctccg cggtcggaat ggcgatggcg
     3421 tcgcgctatg agcgcggcct gttcgacccc gacgccgccc ccggcgagag ccccttcgac
     3481 cactacatct atgtgatcgc ctccgacggc gacatggagg agggggtctc cagcgaggcg
     3541 tcgtcgctgg cgggcaccca gcagctcggc aatctgatcg tgttctacga ccacaatcag
     3601 atttcgatcg agcacgacac caacatcgcg ctgtccgagg atgtgcctgc ccgctaccgc
     3661 gcctacaaat ggcacgtcca ggaggtcgag ggcggtgaga acgtcactgg gatagaagaa
     3721 gccatcgagg ccgcaaaagc agtcaccgac aagccgtcgt tcatctccgt tcggacgatc
     3781 atcggttatc cggcccccac caagatgaac accggcgctg cgcacggctc ggcgctcggc
     3841 gacgaggagg tggccgccac caagcgcatc ctcggcttcg atccggacaa gacgttcgag
     3901 gtgcgcgacg aggtcatcga gcacacccgc aagctggtcg cccgcggcaa ggaagcccac
     3961 gagaagtggc agccacagtt cgacgcgtgg gccgagcggg aacccgagcg caagaagctg
     4021 ctcgaccggc tgttggcaca ggaactgccc gacgggtggg acgccgacat cacgcactgg
     4081 gaacccggct ccaaggccct ggccacccgg gccgccttcg gccaggttct caacgacgtc
     4141 gcacccaagc tgcccgaact gtggggtggc tcagccgatt tggccggcag caacaacacc
     4201 accatcaagg acgtgaagtc gttcggcccg ccgtccattt cgaccgagga cttctccgcc
     4261 gactggtacg gacgcgtgct gcacttcggc atccgcgagc acgccatggg gtcgatcctg
     4321 tccggcatcg tgctgcacgg gccgacccgc gcgttcggcg gcaccttcct gcagttctcc
     4381 gactacatgc ggcccgcggt gcggctggcg tcgttgatgg acatcgacac catctacatc
     4441 tggacccacg actcgatcgg cctcggcgag gacggtccga cccaccagcc catcgagcac
     4501 ctcgcggcgc ttcgggcgat tcccaacctg tcggtggtac gccccggcga cccgaacgag
     4561 accgcctatg cgtggcgcag catcctggcg cgcggcaacg gcagcggccc ggtcgggttc
     4621 gtcctcactc gccagggcat tccggtgctc gagggcaccg acagcgaggg tgtcgccaag
     4681 ggcggctacg tgctcggcgg catccccgac gaactgcccg atgtcgtgct gatcggcacc
     4741 ggatcagagc tgcaactcgc tgtcgaggcg cagaagttgt tggcggagaa ggacatcacg
     4801 gccaccgttg tgtccatgcc gtgcgtcgag tggttcgaat cgcagccggc cgaatatcgc
     4861 gacaaggtgc tgccgcctgc ggtgtcggcc cgagtggcgg tggaggccgc cgtcgcgcag
     4921 agctggtaca agctcgtcgg cgacaccggc gagatcgtct cgatcgagca cttcggcgaa
     4981 tccgccgacg acaagacgct gttccgcgag ttcgggttca ccccggaagc cgttgtggcg
     5041 gccgcggaac gatcactgga caattgatta gagagaagga ccaagcatgg ctcagaatcc
     5101 gaacctcgcc gcgctgtccg ccgcgggcgt ctccgtatgg ctagacgacc tttcccgcga
     5161 ccgcttgcgc accggcaacc tgcaggagct catcgacacg aagtgcgtgg tgggtgtcac
     5221 caccaacccg agcattttcc aggcagcact gtcgaagggc cacgcctacg acgatcagat
     5281 cagggagctg gccgaacgcg gggccgacgt cgacgccacc atccgcacga tcaccaccga
     5341 cgacgtccgc gacgcctgcg atgtgctgcg gccgcagtgg gaggcctccg acggggtcga
     5401 cggccgagtg tcgatcgagg tcgacccgcg gctggcgcat gacaccgata agacggtcca
     5461 gcaggccatc gaactgtgga agatcgtcga ccggccgaac ctgttcatca agatcccggc
     5521 catgatggag ggccttcccg ccatcacctc cgttctcgcc gagggcattt cggtgaacgt
     5581 gacactgatc ttctccgtgg aacggcaccg cgaggtgatg gacgcctacc tggccggcct
     5641 ggaagccgcc aagcagggcg gctacgacct gtccaagatc cactcggtcg cttccttctt
     5701 cgtctcccgc gtggacgccg agatcgacaa gcgactggag aagatcggca gcgacgacgc
     5761 gcttgcactg cgggggcagg ccggcgtcgc caatgcgcgg ctggcatacg ccgcctacca
     5821 agaggtattc gtcggcggtg accgtttcga ggcgctcaag ggtgacggag cgcgggtgca
     5881 gcggccgttg tgggcgtcca ccggcgtgaa gaaccctgag tactccgaca cgctctatgt
     5941 caccgaactg gtcgcgccca acactgtgaa cacgatgccg gagaagacgc tcgaggcggt
     6001 cgccgaccac ggagtcatca ggggtgacac ggtcaccggt accgccggtg ccgcgcagga
     6061 agtcttcgac aagctcgagg ccgtcggcat cgacctgcgg gatgtcttcc tcaccctgga
     6121 gaacgagggc gtcgagaagt tcgagaagtc gtggcaggag ctgcttgaag agacccggac
     6181 tcaactcgac gacaagaaat gagcgcggag ggcaagtccg tcgagacctc cgcggcaccc
     6241 gctgattggc gtaatccgtt gcgcgacaag cgcgacaagc gcatgccccg gattcccggc
     6301 ccgtgcagtg tggtcatctt cggggtcacg ggtgacctgg cccgcaagaa gctgataccc
     6361 gcgatctacg atctggccaa tcgtggtctg ctgcctgcga acttcggcct ggtcgggttc
     6421 gcccgaaggg attgggcaga cggagacttc agcaagctcg tctgcgactc cgtccagcag
     6481 catgcccgga cgccgtatcg gcaggaggtg tgggaccggc tggccgaagg tatccgcttc
     6541 gtacagggca cattcgacga cgagaaggcg ttccaacggc tggccgagac gctggagaag
     6601 ctcgatgccg agcggggtac cgagggaaat cacgcgttct atctgtccat cccgccgaac
     6661 gcgtttcaga tggtctgcga gcaactccag aactccgggt tggcgcgccc gcagccgggc
     6721 cgttggagcc gggtggtgat cgagaaaccg ttcggccacg acctggcgag cgctaaggag
     6781 ttgaacaaca ccgtcaacag cgtcttcccc gaggagtcgg tgttccgcat cgaccactat
     6841 ctgggcaagg aaaccgtaca gaacatcctg gcgttgcggt tcgccaacga actgtacgaa
     6901 ccgatctgga acaac
//
LOCUS       DASYLX010000193         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2312936,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000193 DASYLX010000000
VERSION     DASYLX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312936"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGP27_04645"
     CDS             <1..738
                     /locus_tag="VGP27_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV7850642.1"
                     /translation="GSKAVAVEGDISARSTADELVATAEGLGGLHIVVNNAGITRDKM
                     LFNMSDEDWDAVIAVHLRGHFLLTRNAATYWRAKAKEGDGSVYGRIINTSSEAGLTGP
                     VGQANYGAAKAGIVALTLSAARALERYGVRANAIAPRARTAMTADVFGDAPELAEGQT
                     DSLSPDHVVSLVRFLASPASDRVSGQVFIVYGPTVTLLAAPTAERQFTADGDAWVPVE
                     LSATLRDYFADRELEHGFSATHLMGNP"
     gene            1002..>1622
                     /locus_tag="VGP27_04650"
     CDS             1002..>1622
                     /locus_tag="VGP27_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850643.1"
                     /translation="MIEQLAAPARAVGGFVEMSIDTFVKIFRRPFQFREFLEQTWMIA
                     RVSLIPTLLVAIPFTVLVAFTLNILLREIGAADLSGAGTAFGTITQLGPVVTVLVVAG
                     AGATAICADLGARTIREEMDAMEVMGISPVQRLVVPRVHAAMGVAVLLNGLVSTVGVV
                     GGYFFNVVLQHGTPGAYLASFSALAHLPDLWISEIKAIIFGFIAGVV"
BASE COUNT          269 a          530 c          526 g          297 t
ORIGIN      
        1 ggatcgaagg ccgtcgcggt cgagggtgac atcagtgcgc gcagcaccgc cgacgaactc
       61 gtcgccaccg cagaaggtct tggtggcctc cacatcgtcg tcaacaacgc gggcatcacc
      121 cgcgacaaga tgctgttcaa catgtccgac gaggactggg acgccgtcat cgccgtgcac
      181 ctgcgcgggc acttcctgtt gacccgcaac gccgccacct actggcgggc caaggccaag
      241 gagggcgacg gctcggttta cggccggatc atcaacacgt cctcggaggc ggggctgacc
      301 ggtccggtcg gccaggccaa ctacggcgct gcgaaggccg gcatcgttgc gctgacgctg
      361 agcgcagcca gggcgctgga gcgctacggc gtgcgggcga acgccatcgc tccgcgggca
      421 cgcaccgcga tgacggccga tgtgttcggc gatgcgccgg aactcgccga gggacagacc
      481 gacagcctct cgccggatca cgtcgtctca ctcgtccgct ttctggcgtc gcccgcatcc
      541 gaccgcgtca gcggacaggt cttcatcgtg tacgggccga cggttacgct gctggcggcg
      601 ccgaccgccg agcggcaatt caccgcggac ggtgacgcgt gggtgcctgt cgagctgagc
      661 gcaacgctgc gtgattactt tgctgaccgt gaactggagc acggattctc tgccacccac
      721 ctcatgggaa atccctaaga gcccgctgag agccggggca ctttggtcgc tagaacacgt
      781 tccaggatga tctggatcac acccgtcctg acctggacaa aagctgcaaa tttgtctcaa
      841 tccgggatcc tttgacactg cgaacttgtt ctagttagta tgatccggct cacttagaat
      901 tacgtgcgac gcaggggtgg agaagtcgcc acggcaagca atttccaagg cttcgccatg
      961 gtgaaatccc acgaaacccc gccccgagaa cggagccgag gttgatcgaa cagcttgcgg
     1021 ccccggcgcg ggccgtgggc ggtttcgtcg agatgtccat cgacacgttc gtcaagattt
     1081 tccgcaggcc gtttcagttc cgcgaattcc tcgagcagac gtggatgatc gcgcgcgtct
     1141 cgctgattcc gaccctgctc gtcgccattc cgttcaccgt cctggtggcc ttcactttga
     1201 acatcctcct acgggagatc ggtgcggcgg acctgtccgg cgccggcacg gcgttcggca
     1261 ccatcaccca gctcggtccc gtggtgacgg tgctcgtggt cgcgggcgcc ggagcgaccg
     1321 cgatctgcgc cgacctgggc gcccgcacca tccgcgagga gatggatgcg atggaggtga
     1381 tgggcatctc gccggtccag cgcctggtcg tgccgcgggt gcacgccgcg atgggcgtcg
     1441 ccgtgctgct caacgggctg gtctccactg tgggcgtggt cggtgggtac ttcttcaacg
     1501 tcgttctgca gcacggcaca ccgggggcgt acctggccag tttctccgcc ctggcgcacc
     1561 tgcccgacct gtggatcagc gagatcaagg cgatcatctt cggcttcatc gccggggtgg
     1621 tc
//
LOCUS       DASYLX010000194         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3628845,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000194 DASYLX010000000
VERSION     DASYLX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3628845"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGP27_04655"
     CDS             <1..634
                     /locus_tag="VGP27_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV7850644.1"
                     /translation="GQMLELQGLGGVYSEIFLPLHGEHQAHNAVVALAAVEAFFGAGA
                     DRQLDIDAVRAGFAAAASPGRLERMRSAPTVFIDAAHNPAGAAALAQTLGEEFDFRML
                     VGIVSVMADKDVDGILAALEPVLDQIVVTNNGSPRALDVEGLAVRAEERFGPERVIAA
                     PNLADAIETATALAEEAGHDGEGFSGSGIVITGSVVTAGAARTLFGRDPQ"
     gene            631..1047
                     /locus_tag="VGP27_04660"
     CDS             631..1047
                     /locus_tag="VGP27_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016891538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEV7850645.1"
                     /translation="MSDGPHLPPSSAGHLPPDPWKSFRGVMAGTLILEAIVVLLALPV
                     VAVADGNLTAVTGTYLIGLAVVLIALAGLQGRPWAIWVNLAIQLVLIAGWAVSGAVGF
                     IGLIFTGVWLLIFYLRSEVKRREERGLLPGQQPPHE"
     gene            1154..>1837
                     /locus_tag="VGP27_04665"
     CDS             1154..>1837
                     /locus_tag="VGP27_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850646.1"
                     /translation="MNNPRDFGRPPLDGAPTMAAPVAGQRPGTWPQQASYGDPAPYPP
                     YGRQPPDTYYAQQPPTKLPVYKRPGVVFGAAAVAAVIAVGALLATWASKEGVSTTPVN
                     NTGTPTQQVLAPPAPAPPSAPPPSQSVVVQQPAPRSVPRQQAPAPRVAPSNPSNAPAV
                     SPAPASSLPNSPVPEPIPEPEQKQDPEPQQKQDPPDKFNPGDPENPTPDDLLCHPNPP
                     GCTGDQKAPA"
BASE COUNT          296 a          650 c          616 g          275 t
ORIGIN      
        1 cggccagatg ctcgaattgc aaggcctcgg cggcgtttac tcagagatct tcctaccgct
       61 gcacggcgaa catcaggcgc acaacgccgt cgtcgcgctg gccgccgtcg aggcgttctt
      121 cggtgcggga gcggatcgcc agctcgacat cgacgcggtg cgggccgggt tcgcggccgc
      181 cgccagcccg ggccgtctgg aacggatgcg cagcgcgcca acggttttca tcgacgcggc
      241 ccataacccg gccggcgcag cggcgctggc gcagacgctg ggcgaggagt tcgacttccg
      301 catgctcgtc gggatcgtgt cggtgatggc cgacaaggac gtcgacggca tcctcgccgc
      361 gctcgagcca gtgctcgacc agatcgtcgt caccaacaac ggttcaccgc gcgcgctcga
      421 tgtggagggc ctcgcggtgc gcgccgagga gcggttcgga ccggagcggg tgatcgcagc
      481 gcccaacctg gccgacgcga tcgagacggc gaccgcactc gccgaggagg cgggccacga
      541 cggcgaaggc ttctccggta gcggcatcgt catcaccggg tcggtcgtca ccgccggcgc
      601 cgcccgtacg ctgttcggtc gggatccaca atgagcgacg gtccccatct gcccccctct
      661 agtgcgggac acctgccgcc ggacccgtgg aagagcttcc gcggcgtgat ggcaggcacg
      721 ctgatcctcg aggccatcgt cgtgctgctc gcgctgccgg tcgtcgcggt ggccgacggc
      781 aacctcaccg cggtcaccgg cacctacctg atcgggctgg cggtcgtgct gatcgcgctg
      841 gccggactgc agggccgacc gtgggccatc tgggtgaacc tcgccatcca gttggttctc
      901 atagccgggt gggccgtgtc cggagcggtc ggcttcatcg gcctcatctt caccggcgta
      961 tggctgctga tcttttacct ccgcagcgaa gtgaagcgcc gtgaggagcg tggcctgctg
     1021 cctggccaac agcccccgca cgagtaatta cggcgaacaa aattggggta gcgaactacc
     1081 ctgcacgacc gcagtggaag tgccattgtt gtgccagcgg accgcatgaa tgcaatgcgc
     1141 atgggaagca cagatgaata acccacggga tttcggccgg ccgcccctcg atggggctcc
     1201 gacgatggcc gcgccggtcg ctggtcagcg gcctggcaca tggcctcaac aggcgtccta
     1261 cggcgacccc gctccgtatc cgccctacgg ccggcagccg ccggacacct actacgccca
     1321 gcagccgcca acgaagttgc cggtgtacaa gcgccccggc gtcgtcttcg gggccgctgc
     1381 agtggccgcc gtgatagccg tcggcgccct gttggcgacg tgggcctcga aagagggcgt
     1441 gtcgaccacg cctgtcaaca acacgggcac gccgacgcag caggttctgg ccccgccggc
     1501 gcctgcgccg ccatcggcgc caccgccgag ccagtcggtg gtcgtgcagc agccggcacc
     1561 gaggtcggtc cctcggcagc aggcgcccgc gccccgagtc gcgccctcga atccgtcgaa
     1621 tgcgccggcg gtcagtcccg caccggcatc ttctctacct aattcgcctg tgcccgaacc
     1681 cattccggag cctgagcaga agcaggaccc ggagccgcag cagaagcagg atccgccgga
     1741 caagttcaat ccgggcgatc ccgagaaccc aacgccggac gacctgctgt gccatccgaa
     1801 cccgccgggc tgcacaggag atcagaaggc gccggca
//
LOCUS       DASYLX010000195         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3739278,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000195 DASYLX010000000
VERSION     DASYLX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3739278"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGP27_04670"
     CDS             <1..138
                     /locus_tag="VGP27_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850647.1"
                     /translation="PFHLDLYRAGWFERYIRSFGGTIAGGTSEIQRNIVAQRLLGLPR
                     N"
     gene            complement(306..1058)
                     /locus_tag="VGP27_04675"
     CDS             complement(306..1058)
                     /locus_tag="VGP27_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850648.1"
                     /translation="MLSSSGVELDAALTELGWAEMLSEMADAAVPLVFRLLGETGSHA
                     SVLVDVVLHATGNEIGATVELPLPYAGNSWVVWDRTGGTGTGTALGGLPLRREEEGYP
                     IRVAEARLAVGWWLVGSARAMLSLARQHALDRVQFGKPIASFQAVRHRLAETLVAIEG
                     AEATLSLPGEDNPDLTALLAKAAAGKAALTAARHCQQVLGGIGFTAEHDLHHHVKRVL
                     VLDGLLGSSKELTRKAGAGLRARGSAPRLATL"
     gene            complement(1112..2236)
                     /locus_tag="VGP27_04680"
     CDS             complement(1112..2236)
                     /locus_tag="VGP27_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850649.1"
                     /translation="MTDLFEPAAFRTALQAWLDENDLTPPRGEESLDAHQAQHLRVLK
                     ALYDAGWMRYGWPESAGGLGGPDILRAIVGEEIVGRGIDHPGPYSMLDVLAPTMIDYA
                     RPELAAEMVPRLLSGQESWCQGFSEPGSGSDLASLTTRAEHRGESWVINGQKVWTSFA
                     QYATRCVLLTRTGAPGTPNHEAITAFFVDLDSPGVTVRPLRTMHDVDEFCEVYFDDVV
                     VPADRMLGQPGDGWRLAMDLLPYERSTCFWQRIAYLYSRFDALISEVKGFGQAVDSDM
                     GEAYLALHTLRCRSRATQHRLEDGQKLGPDTSIDKVLLAGAEQRLYDTARDLLPGAIE
                     LDDTPWRTEYLYSRAATIYGGTSEVQRNIIARRLLNLGKE"
     gene            complement(2233..2700)
                     /locus_tag="VGP27_04685"
     CDS             complement(2233..2700)
                     /locus_tag="VGP27_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850650.1"
                     /translation="MTQSTRTEDLVEIQQLLAKYAVTITQGDTDGLTSVFTPDGTYSA
                     FGSTYTLARFPELVDAAPKGLFMTGTSLVHLDADEPDKATGTQPLCFIEHSKHDMRIG
                     YYNDTYVRTDDGWRLKTRAMTFIRRSGDHDSGRPHAIGRPEADVADQAAPEAG"
     gene            complement(2678..3943)
                     /locus_tag="VGP27_04690"
     CDS             complement(2678..3943)
                     /locus_tag="VGP27_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850651.1"
                     /translation="MTAPRASGSSPRVKTLKAAGLLDVDAGEIVRPGIVRIEDDRIVG
                     LGGGTEEGEVVDLGEAILLPGLMDMEVNLLMGGRGENPGLSQVQDDPPTRVLRAVGNA
                     RRTLRAGFTTVRNLGLFVKTGGYLLDVALAKAIDAGWIEGPRVIPAGHAITPTGGHLD
                     PTMFAAFMPGVLELTVEEGIANGNDEIRKAVRYQIKHGAQLIKVCCSGGVMSLTGEAG
                     AQHYSDEELRVIVDEAHRRGLRVAAHTHGAEAVKHAVECGIDCIEHGFLMDDEAIKML
                     VDNDRFLVTTRRLAEAMDVSRAPKELQDKAAEMFPKARTSIKAAYEAGVKIAVGTDAP
                     AIPHGKNADELVTLVDWGMPADAVLRAATVVAADLINKPDLGRIAEGYLADVIAVPGD
                     PLADITVTRNVNFVMKGGKVFKNDTVNKD"
     gene            complement(3946..4275)
                     /locus_tag="VGP27_04695"
     CDS             complement(3946..4275)
                     /locus_tag="VGP27_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850652.1"
                     /translation="MPKLPEEFADLEKFSDWCLPTEEQRYSKRLNSTMAEMQELYDAG
                     MERLEDIMVYVDARFPLKGMPEDAKALVHLGQSIVMVSFPVEVWKQPRVLDSGASYIQ
                     LIKEPVV"
     gene            complement(4268..>4639)
                     /locus_tag="VGP27_04700"
     CDS             complement(4268..>4639)
                     /locus_tag="VGP27_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850653.1"
                     /translation="LLFWAPGWYLTYHYWPTGVDTHIFEADLYFVPPTNLRQRLSQEL
                     AAVTFKEYAFQDANTLEATQTQIGTRVVTEFPLCDQEILLRHLHKTAWDYVNRYKASL
                     TNGNGCGSAGSAATEKDHVNA"
BASE COUNT          757 a         1534 c         1519 g          829 t
ORIGIN      
        1 ccgtttcacc tcgacctcta ccgggccgga tggttcgagc gatacatccg gtcgttcggc
       61 ggcaccatcg ccggtggcac ttccgaaatc cagcgcaaca tcgtcgccca gcgactgctc
      121 gggctgcccc gcaactaggc ctctgactaa gaggactcac cgtgtacatc ggtgcacgga
      181 cagccatgcg cgagcgcgcg cagatgttca tgaattgcgg cgtgtcggcg agcagacacg
      241 cacgctcgcg gggcagaggt gccccagatc gtcgggggaa aggtgccccc agatcgcggg
      301 agcggttaga gggtggcgag gcgtggtgct gagccgcggg cccgcagccc ggctcccgcc
      361 ttgcgggtga gctcctttga gctgccgagc aatccgtcaa gcaccaggac ccgcttcacg
      421 tgatggtgca ggtcgtgctc ggcggtgaag ccgatgccgc cgagcacctg ctggcagtgt
      481 ctggccgcgg tcagcgcggc cttgcccgcg gcggccttgg ccagcagcgc ggtcaggtcc
      541 gggttgtcct caccgggcag gctcagcgtc gcctcggcac cttcgatggc caccagcgtc
      601 tcggccaacc ggtgccggac cgcctggaac gacgcgatgg gcttaccgaa ctgcacgcgg
      661 tccagcgcat gttgacgggc caggctgagc atcgccctcg ccgagccgac cagccaccag
      721 cccaccgcca gacgggcctc ggccacccgg atcgggtagc cctcctcctc gcggcgtagc
      781 ggcagaccgc cgagcgccgt gccggtgcct gtgccgccgg tgcggtccca caccacccag
      841 gagttgccgg catacggcag gggcaattcc accgtggcgc cgatctcgtt gcccgtcgcg
      901 tgcaaaacaa cgtcgacgag aaccgaagcg tgcgaacctg tttcgccgag caaccggaac
      961 accagaggca ccgccgcgtc ggccatttcg gacagcatct cggcccagcc cagttcggtc
     1021 agcgcagcgt cgagttcaac tcctgagctc gacagcatgg tcctgcgcag tccgtcttcg
     1081 aggaggtcga gttcagaatc cgcagccacg atcactcctt cccgaggttc agtaggcggc
     1141 gggcgatgat gttgcgctgc acttctgagg tgccgccgta gatggtggcc gcacgcgaat
     1201 acaggtactc ggtgcgccat ggcgtgtcat cgagttcgat ggcgccaggc agcagatcgc
     1261 gtgcggtgtc atagaggcgc tgttcggcgc ccgcgagcaa taccttgtcg atcgaggtgt
     1321 ccggacccaa cttctgaccg tcctcgaggc ggtgctgggt ggcccgggat cgacagcgca
     1381 gcgtgtgcag agcgaggtac gcctcgccca tatccgaatc gaccgcttgg ccaaagcctt
     1441 tcacctcgct gatcaacgcg tcgaaacgcg agtagaggta cgcgattcgc tgccagaagc
     1501 aggtcgagcg ttcgtagggc agtagatcca tcgccagccg ccagccgtca ccgggctgcc
     1561 cgagcatgcg gtcggccggc accacgacat cgtcgaagta cacctcgcag aactcgtcga
     1621 catcgtgcat ggtgcgtagc gggcgcacgg tgactcccgg cgagtccaag tcgacgaaga
     1681 acgcggtgat ggcttcgtgg ttcggggtgc ccggcgcacc ggtcctggtg agcaggacgc
     1741 agcgagtcgc gtactgcgcg aagctggtcc acaccttttg gccgttgatg acccagctct
     1801 cgccgcggtg ctcggcgcgc gtcgtcaagg acgcgagatc gctgccggaa ccgggttcgg
     1861 agaaaccctg gcaccacgac tcctggcccg atagcagcct cggcaccatc tcggcggcca
     1921 gctcgggccg cgcgtagtcg atcatggtcg gggccagcac gtcgagcatc gaatacggtc
     1981 ccggatggtc gattccacga ccgacgatct cctcgccgac gatcgcgcgc aggatgtccg
     2041 ggcctccgag cccacccgcc gactccggcc atccgtagcg catccaaccc gcgtcgtaca
     2101 gcgccttgag cacgcgaaga tgctgcgcct ggtgcgcatc gagagactcc tcaccccgag
     2161 ggggagtcag atcgttctca tcgagccagg cctgtagcgc cgtgcggaat gcggcaggtt
     2221 cgaacagatc agtcatccag cctccggagc cgcctggtct gcgacatcgg cctctggcct
     2281 tccgatcgcg tgtgggcgac cggagtcgtg gtcgccgctg cgccggatga acgtcatggc
     2341 acgggttttc agccgccacc cgtcgtcggt ccgcacgtag gtatcgttgt agtaaccgat
     2401 gcgcatgtcg tgtttggaat gctcgatgaa gcacagcggc tgggtgccgg tcgccttgtc
     2461 gggctcgtcg gcgtcgaggt gcaccagcga tgtgcccgtc atgaataggc ccttgggcgc
     2521 ggcgtcgacg agttcgggga agcgggccag cgtgtaggtg gagccgaagg cgctgtaggt
     2581 gccgtccggg gtgaacaccg aagtcaaacc gtcggtatcg ccctgggtga tggtgactgc
     2641 gtacttggct agcaactgct ggatttcgac gaggtcctca gtccttgttg actgtgtcat
     2701 tcttgaaaac ctttccgccc ttcatgacga agttgacgtt acgggtaacg gtgatgtcgg
     2761 ccaacgggtc tcccggcacg gcgatgacgt cggcgaggta gccctcggcg atgcggccga
     2821 ggtccggttt gttgatcagg tccgccgcga cgacggtcgc cgcgcgcagc accgcgtcgg
     2881 ccggcatacc ccagtcgacc agcgtgacga gttcgtcggc gttcttgcca tgcgggatgg
     2941 cgggtgcgtc ggtgccgacg gcgatcttca ccccggcttc gtaggcggcc ttgatcgacg
     3001 tgcgtgcctt cggaaacatc tcggcggcct tgtcctgcaa ctccttcgga gcccgtgaca
     3061 cgtccatcgc ctctgccagc cgtctggtgg tgacgaggaa ccggtcgttg tcgacgagca
     3121 tcttgatggc ttcgtcgtcc atcaggaagc cgtgctcgat gcagtcgatt ccgcattcga
     3181 cggcgtgctt gaccgcctcg gcgccatggg tgtgcgcggc cacccgcagc ccgcggcggt
     3241 gcgcctcatc gacgatgacc cgcagctcct cgtccgaata gtgctgcgcg cctgcttctc
     3301 cggtcaacga catgacgccg cccgaacagc acaccttgat cagctgcgcg ccgtgcttga
     3361 tctggtagcg caccgccttg cggatctcgt cgttgccatt ggcgatgccc tcttcgacgg
     3421 tcaactcgag cacgcccggc atgaaggctg cgaacatcgt cgggtcgagg tggccgcccg
     3481 tcggcgtgat ggcatgacct gcgggtatca cccgcgggcc ctcgatccag ccggcgtcga
     3541 tcgccttcgc cagcgccacg tcgagcaggt aaccgccagt cttgacgaac aatccgaggt
     3601 tgcgcacggt ggtgaaaccg gcgcgcagcg tgcgcctggc gttgccgacc gcacgcagca
     3661 cacgcgtggg cggatcgtcc tggacctggg acagaccggg gttctcgccg cggccgccca
     3721 tcaggaggtt gacctccatg tccatcaaac cgggcagcag aatcgcctcg ccgagatcga
     3781 cgacctcacc ttcctcggtg ccaccgccga gaccgacgat ccggtcgtcc tcgatgcgca
     3841 cgatgccggg ccggacgatc tcgccggcgt ccacgtcaag cagacccgct gccttgaggg
     3901 tctttacacg cggcgatgag ccgctcgcgc ggggagcagt caatctcaga ccaccggctc
     3961 cttgatcagt tggatgtagg aggcgccact gtcgagcacc cgtggctgtt tccacacctc
     4021 gacagggaag ctcaccatga cgatcgattg tcccagatgc accagcgcct tggcatcctc
     4081 cggcatcccc ttgagcggga agcgcgcatc gacgtagacc atgatgtcct cgagacgctc
     4141 catgcccgcg tcgtagagtt cctgcatctc ggccatcgtg gagttcagcc gcttggagta
     4201 gcgctgctcc tcagtgggca ggcaccagtc gctgaacttc tccagatcgg cgaattcctc
     4261 tggcagctta ggcattgacg tggtccttct cggtcgcggc ggaaccggcg ctgccgcaac
     4321 cgtttccgtt cgtcagcgac gccttgtacc tgttcacgta atcccaggcg gtcttgtgca
     4381 ggtggcgcag caggatctct tggtcgcaga gcgggaattc ggtaacgacc cgggtgccga
     4441 tctgcgtctg cgtggcctcc agggtgttgg cgtcctggaa cgcgtactcc ttgaacgtca
     4501 ccgcggcgag ctcctgggac agccgttgac gcaggttcgt cggcggcacg aagtagaggt
     4561 cggcctcgaa gatgtgggtg tccaccccgg tcggccagta gtggtacgtg aggtaccacc
     4621 ccggcgccca gaacagcag
//
LOCUS       DASYLX010000196         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_118711,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000196 DASYLX010000000
VERSION     DASYLX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118711"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /gene="groEL"
                     /locus_tag="VGP27_04705"
     CDS             <1..220
                     /gene="groEL"
                     /locus_tag="VGP27_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383248.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV7850654.1"
                     /translation="ELPAGHGFNAATLEYGDLVAGGVVDPVKVTRSAVLNAASVARMV
                     LTTETAIVEKPAEEADGHGHGHGHGHHH"
     gene            402..1277
                     /locus_tag="VGP27_04710"
     CDS             402..1277
                     /locus_tag="VGP27_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850655.1"
                     /translation="MPEHIADEAYRVVRLRDGRQLSYVQYGDPDGFPILNAHGGLACR
                     LDVASAAQAAEMCGVKLVSPDRPGVGRSDPQPGRTILDWARDATELLDRLEADRFAVM
                     GWSLGGQYAAAVGYAWPERVTRVAIIAGALPLTEPNAFDRLPIIDRTYIRLSHYAPWA
                     ARLSFRTMGLAARRAPRLYGWLAASELGTADAAVLRTDGFTTFAHMSGEALRQPQGVV
                     EEYRVMMRPWGFAPEDIVVPTDIWAGADDQLMDPSWPAELARRIPGATLHLRRGGHFM
                     AHLYYREIFESLRCS"
     gene            1803..>2408
                     /locus_tag="VGP27_04715"
     CDS             1803..>2408
                     /locus_tag="VGP27_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850656.1"
                     /translation="MSTWLITGCSAGLGRALAEATINAGHNVVVTARDVAKVTDLAEV
                     NPPQVLAVALDVTRPDQIADAVQKAEQRFGGVDVLVNNAGYGYRAAVEEGDDADVRTL
                     LETHFFGTVAMIKAVLPGMRARRSGAIVNVSSIGAQITPVGSGYYSAAKSAIEGLSGS
                     LRGELAPLGISVTVVEPGAFRTDFAGRSLTQSATVIDDYAET"
BASE COUNT          415 a          804 c          779 g          410 t
ORIGIN      
        1 cgagctgccg gccgggcacg gcttcaacgc cgcgacgctc gagtacggcg acctggtcgc
       61 cggcggtgtc gtcgacccgg tgaaggtcac ccgctcagcg gtgctcaacg ccgcatcggt
      121 ggcgaggatg gtgctcacca ctgagaccgc aattgtcgag aagcctgccg aggaggcaga
      181 cggccacggc cacggtcacg ggcacggcca ccaccactga gtgagcagta cagaacgccc
      241 ccggccttcg gtcgggggcg ttctgtctca tgcgcgaaga gccctcggcc tcggtgcgtc
      301 agtgcatggt tccgcagcat cgatttccct cccgcgacct ggcggtgatg ccacgatgac
      361 gggtggcggg tccgccggtc agcaaccaca cgaggtaggt catgccagaa cacatcgccg
      421 acgaggcata ccgcgtggtc cggttgcgcg acgggcggca actgagttac gtgcaatatg
      481 gcgacccgga cggtttcccg atactcaatg cgcacggcgg actggcctgc cgcctcgatg
      541 tggccagcgc cgcgcaggcc gcagagatgt gcggggtcaa gctggtctca cccgatcgac
      601 cgggcgtcgg ccgttcggat ccgcaaccgg ggcgaacaat cctcgactgg gcccgagacg
      661 ccaccgaact gcttgaccgg ctggaagccg accggttcgc cgtgatgggt tggtcccttg
      721 gcgggcagta cgccgcagct gtcggctacg cgtggccaga gcgcgtaacg cgggtcgcca
      781 tcattgctgg tgcgttgcca ctgaccgaac cgaatgcttt cgatcgtcta ccgatcatcg
      841 atcgcactta catccgcctc tctcactacg cgccgtgggc cgcgcggctt tcctttcgca
      901 ccatggggct ggcggccagg cgcgctccgc ggctatacgg ttggcttgcc gcaagcgaac
      961 tcggaaccgc ggacgccgcg gtactgagga cagacggttt cacgacattc gcacacatgt
     1021 ctggagaggc attacgccaa ccgcagggtg tcgtagagga ataccgggtc atgatgcgcc
     1081 cgtggggatt tgcgcccgaa gacattgtag tgcccacgga catatgggcg ggtgctgatg
     1141 accagctcat ggatccgtcg tggccggccg aactggcacg tcgcatacct ggcgccacgc
     1201 tgcacttgag acgcggaggg cacttcatgg cgcacctgta ctaccgggag atcttcgagt
     1261 cgctgcgctg ttcttgagcc ggcgccggta gtgaagcgat cacgccccga gaatccgatc
     1321 ctcctcactg accagaccgt ttacgccctg acttctcggc cgcctggact gaccgaccgg
     1381 ggtgttcatg cgctgaaggg caagtccgcg cccactgagg ttttcggcct caacccagcc
     1441 gtcacgcctt cgcatcgcct cggctgacgc ttgagcgcac acctaggtac ggtgtcaggc
     1501 cgcaagtttg tacctcagtg agcgctcggc ccccgccgcg gctacaaata ggcgccctcc
     1561 cggcgggacg gaggctgaac cacggcgaga ccctggccaa cggctcgatc acatgcagga
     1621 gcgaaccagc cggaattacc tgtaccgacc tcggcaccgg tcagttcttc cggatagcgc
     1681 gggagtccag ttgaggtgac gcgcagaatc gcgtgagccg cgagcgcgca tcctcgtacg
     1741 gttcagggcg caaaagctgt accagcgtgt gcgctcggcc cggccgagga gagaatcgac
     1801 ctatgtcaac ctggcttatc accggctgct cggcgggctt gggccgcgca ctcgccgaag
     1861 cgaccatcaa cgcgggccat aacgtcgttg tcaccgcgcg tgacgtcgcg aaagtgaccg
     1921 acctcgccga ggtcaacccg ccacaggtgc tggccgtggc cctcgacgtc acccgacccg
     1981 atcagatcgc cgacgccgtg cagaaggccg aacagcgctt cggtggtgtg gacgttctgg
     2041 tcaacaatgc cggctacggc taccgcgccg ccgtggaaga aggcgacgac gccgacgtcc
     2101 ggactttgct cgagacacat ttcttcggca ccgtcgcgat gatcaaagcg gtgcttcccg
     2161 gcatgcgtgc ccgccgttcg ggcgccatcg tcaacgtctc gtcgatcggg gcgcagatca
     2221 ctccggtggg ttcgggctat tactccgcag ccaagtccgc gatcgaagga ctcagcggtt
     2281 cactgcgcgg cgagcttgcg ccgctgggca tttcggtgac cgtcgtcgag ccgggcgcgt
     2341 ttcgcaccga cttcgccgga cgctcgctga cccagtccgc caccgtcatc gacgactacg
     2401 ccgagact
//
LOCUS       DASYLX010000197         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_118716,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000197 DASYLX010000000
VERSION     DASYLX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118716"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP27_04720"
     CDS             complement(<1..306)
                     /locus_tag="VGP27_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7850657.1"
                     /translation="MTAGTAHSGIREIDTGALPDRYARGWHCLGPVRDYLDGKPHGIE
                     IFGTMLVVFADSQGDVKILDGYCRHMGGNLSQGTIKGDEVACPFHDWRWGGDGKCKLV
                     "
     gene            406..948
                     /locus_tag="VGP27_04725"
     CDS             406..948
                     /locus_tag="VGP27_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEV7850658.1"
                     /translation="MPLYTFEGRSPTVDPGAFIAPTATLVGDVHVEAGASVWFNAVLR
                     ADFAPIIVREGANVQDCCVLHAPPGIPVDVGPGATVAHGCVIHGVHIGSEAVIANHST
                     VLDGAVIGSRSLIAAHSLVVGGTKIPDEVVAAGSPAKIRGPVAGTGAQMWVDTNPGAY
                     QDLARRYVAGLREITEPGTP"
     gene            complement(945..1376)
                     /locus_tag="VGP27_04730"
     CDS             complement(945..1376)
                     /locus_tag="VGP27_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850659.1"
                     /translation="MTGPHPLRAPADSPVHLAGKRSREAAVARDKEAWLAVFADDAIV
                     EDPVGPSHFDPEGTGHRGKEAIARFYDMAIAPSELTFNFDKTYQCGNEEANVGNIVIR
                     SSGFEVVAEGVFTYRVNDQGQIIALRAYWELDAATKSARKI"
     gene            complement(1373..2251)
                     /locus_tag="VGP27_04735"
     CDS             complement(1373..2251)
                     /locus_tag="VGP27_04735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7850660.1"
                     /translation="MKYTCAVPMCQIDHLVEIAKTAEEVGFDSIALPDSIFYMEKQSA
                     DYPYTPDGSRMWDENTPWVDPLIAAGAMGAVTSSIRFYTNVMKLGSRNPLLLARQVGS
                     VANLTNNRFGFGVGIGWAPEEFEWCGVPYAKRGKRVDEMIDVIKLVLAGGMVEFHGDF
                     YDFDKLQMSPAPSAPVPFYVGGHTDVALRRAVRVGDGWTSAMLTCDQLGEIIGKLNAL
                     LAEHDRADDPFEFQAVCVDKFGVDGHRELADVGVTDYIGMPWVFDGHGFDAALEKKKD
                     SLKRFADTYIHSGWQE"
     gene            complement(2257..3438)
                     /locus_tag="VGP27_04740"
     CDS             complement(2257..3438)
                     /locus_tag="VGP27_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEV7850661.1"
                     /translation="MAKQLAAVLGTGQTKYVAKRKDVSMNGLVREAIDKALADSGSTF
                     DDIDAVVVGKAPDFFEGVMMPELFMVDAMGATNKPLIRVHTAGSVGGSTAIVATSLVQ
                     SGKYHRVLTMAWEKQSESNAMWALSIPVPFTKPVGAGAGGYFAPHVRAYIRRSGAPTN
                     IGAIVAVKDRLNGAKNPLAHLHQPDITVEKVMESPMLWDPIRYDETCPSSDGAAAMVI
                     GDERSADARVADGHPVAWIHATALRTEPLAFAGRDQVNPQASRDAAAALWKAAGITSP
                     IDEIDVAEVYVPFSWYEPMWLESLGFAPEGEGWKLTEAGETAIGGRIPFNPSGGVLSS
                     NPIGASGMIRFAESAIQVMGKAGEHQVEGARKALGHAYGGGAQYYSMWVVSADKPADK
                     P"
     gene            complement(3470..4540)
                     /locus_tag="VGP27_04745"
     CDS             complement(3470..4540)
                     /locus_tag="VGP27_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEV7850662.1"
                     /translation="MRGFTVTDVAVVGFAHAPHVRRTDGTTNGVEMLMPCFASIYAEL
                     GLKQTDIGFWCSGSSDYLAGRAFSFISAIDSIGAWPPINESHVEMDAAWALYEAYIKL
                     LTGEVETALVYGFGKSSAGTLRRVLALQTDPYTVAPLWPDSVSMAGLQARFGLDAGKW
                     TAEQMAQVALESMAAAGRTDSEKPAKSIDELLSRPYFADPLRRHDIAPITDGASAIVL
                     AAGDKARELRENPAWITGFEHRIETPVLGARDLTTSPSTAASAQGATGGDAASIEVAE
                     LYAPFSHQQLILREAIGLSDSAKMNPSGGALSANPMFSAGLERIGFAARHIFDGLAGR
                     VLAHATSGPALQQNLVAVMEGR"
     gene            complement(4537..5535)
                     /locus_tag="VGP27_04750"
     CDS             complement(4537..5535)
                     /locus_tag="VGP27_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7850663.1"
                     /translation="MTTSQSSPVQIDTHEPPLSAPLKLSFDYTRSVGPLLSQFFTALR
                     GRRIVGVRGSDGRVLVPPAEYDPVTYEALTEVVPVATVGTVLSWTWQAHPLEGQPLDR
                     PFAWALIKLDGADTALLHAVAADSSEEISTGARVHVHWVDEPVGAITDIAYFALGDTE
                     ESEQPSLGSDDRDPVTIAVMPSSIEIQHTASLPETAYLKALKEGKLLGGRSGADGKLY
                     FPAREADPATGKVLDQFVELPDEGTVTTFAIINIPFAGQRIKPPYVAAYVLLDGADIP
                     FLHLVSEIDANEVRMGMRVKAVWRPREEWDFGIDNIEYFRPTGEPDADYDTYKHHL"
     gene            5621..>5949
                     /locus_tag="VGP27_04755"
     CDS             5621..>5949
                     /locus_tag="VGP27_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7850664.1"
                     /translation="MKLGLQLGYWGAQPPENAPELVAAAEEAGFDTVFTAEAWGSDAF
                     TPLAWWGSATSRLRLGTSVVQLSARTPTACAMASLTLDHLSGGRHILGLGVSGPQVVE
                     GWYGQKFP"
BASE COUNT          958 a         1994 c         1934 g         1063 t
ORIGIN      
        1 caccagcttg cacttgccgt cgccgcccca acgccagtcg tggaacgggc aggccacctc
       61 gtcgcccttg atcgtgcctt gcgagaggtt gccccccata tgcctgcagt agccgtcgag
      121 gatcttgacg tcgccctgcg aatcggcgaa gacgaccagc atggtgccga agatctcgat
      181 gccgtgtggc ttgccgtcga ggtagtccct gacggggccg aggcagtgcc aaccacgcgc
      241 gtaccggtcc ggtagcgcgc cggtgtcgat ttcgcgaata ccgctgtgag ctgttccagc
      301 cgtcacgttc gcacctcccg tccttgctct ctaactagaa cacgttacag tttttaccct
      361 cggccgcgca acgtaaacgc catttacctg cggtatatct agacaatgcc tctgtacacg
      421 ttcgagggtc gatccccgac ggtcgatccg ggcgccttca tcgccccgac cgccacgctg
      481 gtcggcgacg tgcacgtcga agcaggcgcg tcggtgtggt tcaacgcggt gctgcgtgcc
      541 gacttcgctc ccatcatcgt ccgtgagggc gcgaacgtgc aggactgttg cgtgctccac
      601 gcgccgccgg gtattcccgt cgacgtcggc ccgggtgcca ccgtcgcgca cggttgtgtg
      661 atccacggcg tgcacatcgg gtccgaggcg gtcatcgcca accattccac ggtgctcgat
      721 ggcgccgtga tcggctcgcg cagcctgatt gcggcgcatt cactggtggt cggcggcacg
      781 aagatccccg acgaagtcgt cgccgccggc tcgcccgcca agatcagggg tccggtcgcg
      841 ggcaccggcg cgcagatgtg ggtggacacc aatcccggtg cctatcagga tctggcccgg
      901 cgttacgtcg ccggcctgcg ggagatcacc gagccgggaa cgccttagat cttgcgggcg
      961 ctcttggtgg cggcgtcgag ttcccagtag gcgcgcaacg cgatgatctg gccctggtcg
     1021 ttcacccggt aggtgaacac gccctcggcg acgacctcga atccgcttga ccggatcacg
     1081 atgttgccga cattggcttc ctcgttgccg cactggtagg tcttgtcgaa attgaatgtc
     1141 aactcgctcg gcgcgatcgc catgtcgtag aaccgcgcga tcgcctcctt gcccctgtgc
     1201 cccgtgcctt ccgggtcgaa gtgcgacggc ccgaccggat cctcgacgat ggcgtcgtcg
     1261 gcgaacaccg ccagccacgc ctccttgtcg cgagcgaccg cggcctcgcg ggagcgcttg
     1321 cccgccaggt gcaccggcga gtccgcgggc gcgcggagcg gatgaggccc cgtcactcct
     1381 gccagcccga gtggatgtag gtgtcggcga accgcttcag cgaatccttt ttcttctcca
     1441 gcgcagcgtc gaatccgtga ccgtcgaaca cccacggcat gccgatgtag tcggtgacgc
     1501 cgacatcggc aagttcgcgg tgcccgtcga caccgaactt gtcgacgcag acggcctgga
     1561 actcgaacgg gtcgtcggca cggtcgtgtt cggccagcaa ggcgttcagc ttgccgatga
     1621 tctccccgag ctgatcacag gtcagcatcg cactggtcca gccgtcgccg acacgcacgg
     1681 cgcgtcgcaa cgccacatcg gtgtgtccgc cgacgtagaa cggcaccggc gcgctgggag
     1741 cggggctcat ctgcagcttg tcgaagtcgt agaagtcacc gtggaactcg accatgccgc
     1801 cggcaagcac cagcttgatg acgtcgatca tctcgtcgac acgcttaccg cgtttcgcgt
     1861 aaggcacgcc gcaccactcg aattcctcgg gcgcccagcc gatcccgaca ccgaagccga
     1921 accggttgtt ggtcaggttg gccaccgatc cgacctgccg ggccagcagc agaggattgc
     1981 gtgagcccag cttcatcacg ttggtgtaga agcgaatgct cgacgtcacc gcacccatcg
     2041 cgcccgcggc gatcagcggg tccacccacg gggtgttctc gtcccacatc cgcgagccgt
     2101 ccggcgtgta cgggtagtcc gccgactgct tctccatgta gaagatcgag tccggcagcg
     2161 cgatcgagtc gaagccgact tcctctgcgg tcttggcgat ttcgaccagg tggtcgatct
     2221 gacacatcgg caccgcgcag gtgtacttca ttttgctcac ggcttgtcgg ccggcttgtc
     2281 ggcggaaacc acccacatcg agtaatactg tgcgccgccg ccgtaggcat ggccgagcgc
     2341 cttgcgggcg ccttcgactt gatgctcgcc cgccttgccc atcacctgga tggccgactc
     2401 ggcgaagcga atcattcccg acgcaccgat cgggttcgac gacagcaccc caccggaggg
     2461 gttgaacgga atgcgaccgc cgatcgcggt ctcgccggcc tcggtcagtt tccagccctc
     2521 gccctcgggg gcgaacccca ggctctccag ccacatcggt tcgtaccagg agaacggcac
     2581 gtagacctcc gcgacgtcga tctcgtcgat cgggctggtg atccctgccg ccttccacag
     2641 cgcagcggcc gcgtcacggc tggcctgcgg attgacctgg tcccggcccg cgaaagccaa
     2701 cggttcggtg cgcagcgcgg tggcgtgaat ccaggcgacg gggtgaccgt cggcaacgcg
     2761 ggcgtcggcc gagcgctcgt cgccgatcac catcgccgcc gcaccgtccg acgatggaca
     2821 ggtctcgtcg tagcggatcg gatcccacag catcggcgac tccatcacct tctcgacggt
     2881 gatgtcgggt tgatgcagat gcgccagcgg gttcttggcc ccgttgagcc ggtccttgac
     2941 cgcgacgatg gccccgatgt tggtcggcgc gcccgagcgg cggatgtacg cccgcacgtg
     3001 gggggcgaag tatccaccgg cgcccgcgcc gaccggcttg gtgaacggca ccggaatgct
     3061 caacgcccac atggcattcg actcagactg cttctcccat gccatggtga gcacgcgatg
     3121 gtacttgccg gactgcacca ggctggttgc gactatcgcg gtggaaccac ccaccgagcc
     3181 cgcggtgtgc acacggatca gcggcttgtt cgtcgcgccc atcgcatcga ccatgaacaa
     3241 ctcgggcatc atgacgccct cgaagaagtc gggcgcctta cccaccacta cggcgtcgat
     3301 gtcgtcgaag gtcgagcccg agtcggccag cgccttgtcg atcgcctcgc gcacaaggcc
     3361 gttcatcgat acgtccttgc gcttggcgac gtacttggtc tgcccggtgc ccaacaccgc
     3421 ggccaattgc ttcgccatca tttatccttc ttctcttcgc gcacgcggct catcgtccct
     3481 ccatgaccgc gacgaggttt tgttgcaggg cggggccgct ggtggcgtgg gcaagcaccc
     3541 gacccgccaa gccgtcgaag atgtgccgtg cggcgaagcc gatgcgctcg agcccggcgg
     3601 agaacatcgg gttcgcgctc agcgctccgc ccgacgggtt catcttcgcg ctgtcggaca
     3661 gcccgatcgc ctccctgagg atcagctgct gatgactgaa cggtgcgtag agttcggcca
     3721 cctcgatcga ggccgcgtcc cctccggttg ccccctgtgc cgaagcggcg gtggacgggg
     3781 aggtggtcag atctcgcgcg ccgagcaccg gcgtctcgat gcggtgctcg aatcccgtga
     3841 tccaggccgg gttctcgcgc agttcgcgcg ctttgtcgcc ggcggcaagc acgatcgcgg
     3901 acgcgccgtc ggtgatcggt gcgatgtcgt ggcggcgcag tggatcggcg aaatagggcc
     3961 gcgagagaag ttcgtcgatg ctcttcgcgg gcttctccga atcggtgcgc ccggcggcgg
     4021 ccatggattc cagcgccacc tgcgccatct gttcggcggt ccacttgccc gcgtcgaggc
     4081 cgaaccgcgc ctgcaggccg gccatcgaca ccgaatccgg ccacagcggc gcgaccgtgt
     4141 agggatcggt ctgcagcgcc agcaccctgc gcagggtgcc cgccgacgac ttaccgaacc
     4201 cgtacaccag tgccgtctcg acttcgccgg tgagcagctt gatgtaggcc tcgtacaggg
     4261 cccatgcagc gtccatctcg acatgggact cgttgatcgg cggccacgcg ccgatcgagt
     4321 cgatcgccga gatgaacgag aaggcgcgtc cggcaaggta atccgacgac ccggagcacc
     4381 agaagccgat gtctgtctgt ttcaggccga gttcggcata gatcgacgcg aagcacggca
     4441 tcagcatctc gacgccgttg gtggtgccgt cggtgcggcg cacatgcggg gcgtgagcga
     4501 agcccaccac tgccacatca gtcacggtga agcctctcag aggtggtgct tgtaggtgtc
     4561 gtagtcggcg tccggttcgc cggtgggccg gaagtactcg atgttgtcga tgccgaagtc
     4621 ccattcctcg cggggcctcc acaccgcctt cacccgcatg cccatccgca cctcgttggc
     4681 atcgatctcc gagaccagat gcagaaatgg gatgtcagcg ccgtcgagca acacataggc
     4741 cgcgacgtac ggcggcttga tgcgctggcc tgcgaaggga atgttgatga tcgcgaaggt
     4801 cgtcaccgtg ccctcgtcgg gcagttcgac gaattgatcg agcaccttgc cggtggccgg
     4861 atccgcctcg cgcgccggga aatacagctt cccatccgcg ccggagcgcc cgccgagcag
     4921 cttgccctcc ttgagcgcct tgagataggc ggtttccgga agcgacgcgg tgtgctgaat
     4981 ttcgatgctg ctcggcatca ccgcaatggt gaccggatcg cggtcgtcgg acccgaggga
     5041 tggctgttct gattcttcgg tgtcaccgag ggcgaaatac gcgatgtcgg tgatcgcgcc
     5101 gaccggttcg tcgacccagt gcacatgcac ccgcgcgccg gtgctgatct cctcggacga
     5161 atccgcggcg accgcatgca gcagggcggt gtcggcgccg tccagcttga tcagcgccca
     5221 tgcgaacggt ctgtccagcg gctgaccctc gagagggtgg gcctgccagg tccacgacag
     5281 cacggtcccg acggtggcca ccggcacgac ctcggtcaac gcctcgtagg tcacggggtc
     5341 atactcagcg ggcggaacaa gcacccgccc atcggagccg cgcacgccga cgatgcgtcg
     5401 accgcgcagg gcggtgaaga actgactcag gagcggtccc actgaacggg tgtagtcgaa
     5461 cgacagcttc agcggcgccg agagtggcgg ctcatgcgtg tcgatctgca ccgggctgct
     5521 ttggctggtg gtcacagcgt cgagtagaac aggttctaag aatggtttca agaacgggtc
     5581 ggaaggcggt ctaaatggac gcaggtttgg ggcggcggga atgaagctgg gattgcagct
     5641 gggatattgg ggcgcgcagc cgcctgagaa tgctcccgaa ctggtcgccg cggccgagga
     5701 ggccggcttc gacaccgtgt tcaccgcgga ggcctggggg tcggatgcct tcaccccgct
     5761 ggcatggtgg ggttcggcga ccagccggct gcggctgggc acctcggtgg tgcagctctc
     5821 cgcgcgcacg ccgaccgcct gcgcgatggc ctcgctgacc ctcgatcacc tgtccggcgg
     5881 ccggcacatc ctcgggctcg gcgtatcggg acctcaggtc gtcgagggtt ggtacggcca
     5941 gaagttccc
//
LOCUS       DASYLX010000198         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3519250,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000198 DASYLX010000000
VERSION     DASYLX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519250"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1714
                     /locus_tag="VGP27_04760"
     CDS             170..1714
                     /locus_tag="VGP27_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850665.1"
                     /translation="MTGYDFIVVGAGSAGCAVAGRLAAESSASVLLVEAGGSDRRLTT
                     RVPLAAPMQYGTSLDWAYQSEPEPGCADRRIAQPRGRVLGGCSAMNGLFWIKGSNLDY
                     DGWRLSGWGWDDVAPVFARIEQGPMHITRSPFPDELSHRFVTSARAAGVAANDDVSGP
                     DLDGAAITPVNIHKGQRWSTARGYLRHQNNLTVVTKACVDRVIIRDGRAVGIEYRRRG
                     RAHQAFADQDVVLSAGAFGTPQLLQVSGIGPADHLRRAGVTPLVDSPRVGQGLADHPL
                     TWAVWSLAPGYVGLADVANPKWLLQWLLGRSGKLTSNGGEALAHIRSTVEAPACDVQL
                     IFTPADAWAEPRGGRFAPALSVGHSYWTPKSRGSVLIRSSDPAVPPAIRMNLLTERED
                     VEALVRAVQRTREIIATEPIASTVERELLPGTGADIEACIRNTAKTTSHPACSVAMGN
                     DADSPLDEKLRVRGVDNLRVADASALPEIPRANTNAPSIMIGERCADFLLALADRGLQ
                     GSVDAQ"
     gene            complement(1907..1980)
                     /locus_tag="VGP27_04765"
     tRNA            complement(1907..1980)
                     /locus_tag="VGP27_04765"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1944..1946),aa:Met,seq:cat)
     gene            complement(1997..>2447)
                     /locus_tag="VGP27_04770"
     CDS             complement(1997..>2447)
                     /locus_tag="VGP27_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7850666.1"
                     /translation="HVPPEALILEITEGTFANDSSRSRKTVFALRRLGVRISLDDYGT
                     GWSSLARLQDLSVAELKLDRIFVSRLAEDPRSIAIVPLAHSFGADLVAEGVEDEATLA
                     ALRRYGCNITEGFVHTPPLPADALLSWIARCTPQVAVSDLALHPDLR"
BASE COUNT          426 a          816 c          782 g          423 t
ORIGIN      
        1 tccagctagt ttcgctcatg ctagccagca tgcggtgacg cgcagcgcag aacatcagcg
       61 atatcgccaa tagttgcgac gcaggcatgc gtacatacgc atccggccat gacggattta
      121 ggcgcaatgg ccgatggtcg ctggtcggcg tcgccggata gtcgacgtca tgacgggtta
      181 tgacttcatc gtggttggtg caggttctgc gggttgcgcg gtcgctggac ggttggcggc
      241 cgaatcctcg gcaagtgtcc tattggtcga agccggtggc tctgatcgac ggttgacgac
      301 acgagtgccg ctggccgcac ccatgcagta cggcacgtcc ttggactggg cgtaccagag
      361 cgaaccggag cccggctgcg ccgaccggcg gatcgcccaa ccccgcggtc gtgtgctcgg
      421 cggctgcagt gcgatgaacg gcctgttctg gatcaagggc agcaatctgg actacgacgg
      481 ctggcggctg tccggctggg gctgggatga cgtcgctccg gtgttcgcgc ggatcgagca
      541 ggggccgatg cacatcaccc gctcaccatt tcccgacgag ctgtctcatc gcttcgtcac
      601 ctcagcgcgg gccgcaggcg tcgctgccaa cgatgacgtc agcggacccg acctcgacgg
      661 tgcggcgatc acgccggtca acatccacaa gggccagcgt tggagcacgg cgcgcggtta
      721 cctccggcat cagaacaatc tgaccgtcgt caccaaggcc tgcgtcgacc gcgtgatcat
      781 ccgcgacggt cgtgccgtcg gaatcgaata ccgccgccgc ggacgtgcgc accaggcttt
      841 cgccgaccag gacgtcgtcc tgagtgcagg cgctttcggc acgccgcagc tgctgcaggt
      901 gtccggtatc ggtccggcgg atcacctccg ccgggccggg gtgaccccac tggtcgacag
      961 cccccgtgtc gggcaaggcc tcgctgatca cccgttgacc tgggcggtat ggtccctcgc
     1021 gcccggttat gtcggcctcg cggatgtcgc gaacccaaag tggttgctgc agtggctgtt
     1081 aggtcgcagt ggcaagctca ccagtaacgg cggggaggcg ctggcccaca tccggtcgac
     1141 ggtcgaagcc ccagcatgcg acgttcagct gatcttcacg cctgccgacg cctgggccga
     1201 acctcgtggg ggaagatttg cgcccgcgtt gtcggttggc cattcgtatt ggacacccaa
     1261 gagccgcgga agtgtcctca ttcgttcatc ggatcccgct gtaccaccgg cgatccggat
     1321 gaacctgctc accgaacgcg aggatgtcga ggcactcgtg cgcgccgtcc agcgcacacg
     1381 cgagatcatc gccaccgagc cgatcgcatc gaccgtcgag cgtgagctgc tgcccggtac
     1441 gggcgcggat atcgaggcct gtatcaggaa caccgcgaaa accaccagcc atcccgcgtg
     1501 cagcgtcgcg atgggcaatg acgctgacag cccgcttgac gagaagctgc gggttcgcgg
     1561 tgtggacaac ctccgggttg ccgacgcctc ggcgctcccc gagatccccc gcgccaacac
     1621 caatgctcct tcgatcatga tcggcgaacg ctgtgcggat ttcctgctcg cgctcgctga
     1681 ccgcggcctg cagggctccg tagacgctca gtgacgcggc cttatcgagc acgacggtat
     1741 cgagcacgac gatcgagtcg gcgggttcga ccggctcccc tgggtccgtc aggctcgttc
     1801 ccgaaatgtt cccattcgat cgtcagcgat gcgaagttcc caaaactgtt cccaacccca
     1861 atgaaaacgg cccccgggag tgatttccgg ggaccgattc ctttagtagc ggggacagga
     1921 ttcgaacctg cgacctctgg gttatgagcc cagcgagcta ccgagctgct ccaccccgcg
     1981 acgggtgaac accacgttac cgaaggtcag ggtgcagcgc caaatcgctc accgcgacct
     2041 gcggcgtgca gcgcgcaatc caggaaagca gcgcgtcagc gggtagcggc ggcgtgtgca
     2101 caaagccctc ggtgatgttg cagccgtacc gacgcagggc agccagagtc gcctcgtctt
     2161 cgaccccctc ggccaccagg tccgccccga aactgtgcgc cagcggcacg atggcgatcg
     2221 accgcgggtc ctcagccagc cgcgatacga agatcctgtc cagcttcagt tcagcgaccg
     2281 acaggtcctg cagcctcgcc agcgatgacc acccggtgcc gtagtcgtcc agcgagatcc
     2341 gcaccccgag gcgccgcagc gcgaacaccg tcttgcgcga tcgcgatgag tcgttcgcga
     2401 aggttccctc ggtgatttcc aggatcagcg cctccggcgg cacatga
//
LOCUS       DASYLX010000199         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_777136,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000199 DASYLX010000000
VERSION     DASYLX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777136"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VGP27_04775"
     CDS             complement(<1..748)
                     /locus_tag="VGP27_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7850667.1"
                     /translation="MVRRPPHEDAYSARLAGVARFAVTHKRIVIGTWLGLAVVLAMLF
                     PQLETVVKQQSLDPIPRDVASFQTLDRMGKAFGEEGSTTTVVVAMEDPEGLTPSARKR
                     YAAMVSALRADSENVPLVQDLLADPVTAGQAMSADGKAWYLPVGIAGTLGGPRAAESV
                     ASVRDIATDAFGGTSTVAYVTGPAATMSDQIVAAERDLLFISLATAGLIAAILLLVYR
                     SVFTALLPLLVIGVSLGVGRGVLSALGELGM"
     gene            899..>1636
                     /locus_tag="VGP27_04780"
     CDS             899..>1636
                     /locus_tag="VGP27_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850668.1"
                     /translation="MQTLGSFDVEGIDFAFDDQPDLHAALARLRERRPYAIVPFAGVR
                     AVLFLTDELVRAAFRDEATFPAAPVYAMTTEPVFGRTVLSMSGAEHRASRSVVSAPLR
                     RTRVQDYLEPVIEPVINELIDQFAARGTADLVVEFTHRYSLLIISRLLGFPVDDEANI
                     HRWAQAMIQYPFDPPAAVTCAAEFTAYVAPLLAARRREPGDDMISMLVTETTEDGENL
                     SDEEILTFLRMLFPLGADTTTLALGNIL"
BASE COUNT          283 a          580 c          516 g          257 t
ORIGIN      
        1 gcatgcccag ttcgccgagc gccgacagga ctccgcgccc gacgcccagg ctcacgccga
       61 tgacgagcaa cggcagcagc gcggtgaaca ccgagcggta gaccagcagc aggatcgccg
      121 cgatcagacc ggcggtcgcc aacgagatga acagcagatc cctttccgcc gcgacgatct
      181 gatcgctcat ggtggcagcc ggacccgtga cataggcgac cgtcgacgtg ccgccgaacg
      241 catcggtggc gatgtcgcgg accgacgcga cggattcggc ggcccgcggt ccacccagcg
      301 tcccggcaat gccgaccggc agataccaag cctttccatc ggcgctcatc gcctggccgg
      361 cagtgaccgg gtcggcgagc agatcctgga ccagtggcac gttctccgag tcggcgcgca
      421 acgcggaaac cattgcggca tagcgcttcc gcgctgatgg ggtcagccct tccggatcct
      481 ccatcgcgac gaccaccgtc gtcgtcgacc cttcctcgcc gaacgccttg cccatccggt
      541 ccagcgtctg aaacgacgct acgtcgcggg gaatcgggtc cagcgactgc tgtttgacga
      601 cggtctccaa ttgtggaaac aacatcgcga gaacgacggc cagacccagc caggtgccga
      661 tgacgatgcg cttgtgcgtg acggcgaacc gcgccacccc ggccaggcgg gcgctgtagg
      721 cgtcctcgtg cggcggacgc ctcaccatgg ccgcgccgcc atcgtccgat cggatctgct
      781 gtcagccatc tcgcctcctg aggacggtcg cagggcgacg gaatcgcgcc ccatgcggac
      841 gcgccgcaac gtgcgacata ttgcgttgag tgttgcacag cggctagggt gcggctgtat
      901 gcagaccctg ggatccttcg acgtcgaggg aatcgacttc gcgttcgacg accaaccgga
      961 cctccacgcc gcgctcgcgc ggctccgcga gcgcaggccg tacgcaatcg ttccgttcgc
     1021 cggggtccgc gcggtcttgt ttttgaccga cgagctggta cgggcggcct tccgcgacga
     1081 agcgacattt cccgccgcac cggtgtacgc gatgaccaca gaaccggtgt tcggcaggac
     1141 cgtgctgagc atgtcgggcg ccgagcatcg cgccagccgc agcgtggtgt cggcgccgct
     1201 gcggcgcacc cgcgtccagg actacctcga gccggtgatc gaacccgtca tcaacgagct
     1261 gatcgaccag ttcgccgccc gcggcaccgc cgatctggtc gtcgaattca cgcaccgcta
     1321 ctcgctgctg atcatcagcc gcctgctggg cttcccggtc gacgacgaag ccaatattca
     1381 ccgctgggca caggcgatga tccagtatcc cttcgacccg ccggcggcgg ttacgtgtgc
     1441 cgccgaattc accgcctacg tcgcaccgct actggccgcg cgccgccggg aaccgggcga
     1501 cgacatgatc tccatgctgg tcaccgaaac cactgaggac ggcgagaacc tctcggacga
     1561 agagatactc acgttcctgc ggatgctgtt cccgctcggc gccgacacga ccacgctggc
     1621 gttgggcaac atcttg
//
LOCUS       DASYLX010000200         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2861374,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000200 DASYLX010000000
VERSION     DASYLX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861374"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VGP27_04785"
     CDS             complement(<1..1285)
                     /locus_tag="VGP27_04785"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850669.1"
                     /translation="MTQTLDPATALTPQERITTWLADFEASLAVRDIDRAVAKFGTDS
                     FWRDLVAFTWNLTTVEGPDQVGAMLGERLAGTAPSNFRTRDEAIEDAGVVSAFIEFET
                     AVGRGVGHLRLKSGPAGDQAWTLLTALQELKGHEERMGPSRVLGAVHGSDHDTRSWAE
                     KRADEEAALGFAAQPYTLVVGGGQGGIALGARLRQLGVPSIVVDRHERPGDQWRKRYK
                     SLCLHDPVWYDHLPYLPFPANWPVFAPKDKIGDWLEFYTRVMEVPYWSKTTCLSASFD
                     EQAKRWTVEVDRDGGRVTLHPTHLVLATGMSGKPSIPTLAGQDVFRGDQHHSSAHPGP
                     DAYVGKKAVVIGSNNSAHDICKALYETGVDVTMVQRSSTHIVKSDSLMELGLGDMYSE
                     RAVAGGMTTEKADLTFASLPYRILHEFQIPVYNAIR"
     gene            1530..3053
                     /gene="adh"
                     /locus_tag="VGP27_04790"
     CDS             1530..3053
                     /gene="adh"
                     /locus_tag="VGP27_04790"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEV7850670.1"
                     /translation="MTVFSRPGSADALMAYESRYGNFIGGEWVAPSGGEYFENPTPVT
                     GQVFCEIPRSTEADIDKALDAAHGAAGAWGKTAAGERAVILNKIADRIEANLESLALA
                     EAWDNGKPIRETLAADLPLAVDHFRYFAGAIRAQEGSLSQIDDDTVAYHFHEPLGVVG
                     QIIPWNFPILMATWKLAPALAAGNAVVLKPAEQTPASILYLMSLIGDLLPAGVLNVVN
                     GFGFEAGKPLASSNRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFSDV
                     MAAGDEFQDKALEGFTMFALNQGEVCTCPSRSLIQSDIYDEFLELAAIRTKAVRQGDP
                     LDTETMIGSQASNDQLEKVLSYIEVGKDEGARVVTGGERAQLGGDLNGGYYVQPTIFE
                     GNNKMRIFQEEIFGPVVAVTSFSDYDDAISLANDTLYGLGAGVWSRDGNVAYRAGRDI
                     KAGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYSNKAQGFF"
     gene            3053..3544
                     /locus_tag="VGP27_04795"
     CDS             3053..3544
                     /locus_tag="VGP27_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEV7850671.1"
                     /translation="MPDAPSRALVTRAAADLLEKLQGKYGALMFHQSGGCCDGSSPMC
                     YPDGDFIVGDRDILLAVLDVGDTGVPVWISGPQFEAWKHTQLVIDVVPGRGGGFSLEA
                     PEGLRFLSRGRAFTDDENRSLADSAPVTGAEYERGLRPAAGGTHVVAEAIDACPIPGT
                     PRR"
BASE COUNT          612 a         1193 c         1230 g          607 t
ORIGIN      
        1 cgcggatcgc gttgtagacc gggatctgga actcgtgcag gatccggtac ggcagcgagg
       61 cgaacgtcag gtcggccttc tcggtcgtca tgcccccggc caccgcccgc tccgagtaca
      121 tgtcgccgag cccgagttcc atcagcgagt ccgacttcac gatgtgcgtc gagctgcgct
      181 gcaccatcgt cacgtcgaca ccggtttcgt acaacgcttt acagatgtcg tgtgcggagt
      241 tgttggagcc gatgacgacc gccttcttgc cgacgtaggc gtcgggccca ggatgcgcac
      301 tggagtggtg ctggtcgccg cggaacacat cctggccggc cagcgtgggg atgctcggct
      361 tgccggacat gcccgtcgcg agcacgagat gcgtcgggtg cagcgtgacg cgtccgccgt
      421 cgcggtccac ctcgaccgtc cagcgcttgg cctgctcgtc gaacgacgcc gacagacacg
      481 tcgtcttcga ccagtacggc acctccatca cccgggtgta gaactccagc cagtcgccga
      541 tcttgtcctt gggcgcgaac acgggccagt tcgccgggaa cggcagataa ggcaggtggt
      601 cgtaccacac cgggtcgtgc aggcacagcg atttgtagcg cttccgccac tggtcacccg
      661 ggcgctcgtg gcggtcgacg acgatgctgg gcacccccag ctggcgcagg cgcgcgccga
      721 gcgcgatacc gccctgtccg ccgccgacga ccagggtgta cggctgggcc gcgaagccga
      781 gggcggcttc ctcgtccgcc cgcttctcgg cccacgaccg ggtgtcgtga tcgctgccgt
      841 gcaccgcacc cagcacacgc gacgggccca tccgctcctc gtgtcccttc aattcctgca
      901 gagccgtcag cagcgtccac gcctgatcgc ccgcagggcc ggacttcagc cgcaggtggc
      961 cgacgccgcg gccgacggcg gtctcgaact cgatgaaggc ggagaccacg cccgcgtctt
     1021 cgatcgcctc gtcgcgagtg cggaagttcg acggggcggt gccggcgagg cgctcgccga
     1081 gcatggcgcc gacctggtca ggtccctcga cggtggtgag gttccacgtg aacgcgacca
     1141 gatcgcgcca gaagctgtcg gtgccgaact tggcgaccgc gcggtcgatg tcgcgcaccg
     1201 ccaatgaggc ctcgaagtcg gcgagccagg tggttatccg ctcctgcgga gtgagggcgg
     1261 tggcggggtc cagcgtctgc gtcatgcgat ccaccacaca cccgatcgcg ccgagtcaca
     1321 agggttgcag cgggtcggcg cccatggcgg tctcgtccac cgctgggggg aggctggcgg
     1381 gaaggtgtga tggagagcac aatcgggact gatgcacggt cagccagtgt ctgagcccgt
     1441 agtggcggtc ggcgaggatg cgcgtccgcg tttcatccgc actggacacg aaaagtagtg
     1501 attatcaaca actctcgaag gagtcagtca tgaccgtatt ctcccgtccg gggtccgccg
     1561 acgctctgat ggcctacgag tcccgctacg ggaacttcat cggcggcgag tgggtggcgc
     1621 cgtcgggtgg cgagtatttc gagaacccca cgccggtgac cggtcaggtg ttctgcgaga
     1681 tcccgaggtc aaccgaggcc gacatcgaca aggccctcga cgccgcgcac ggtgcggccg
     1741 gtgcgtgggg caagacggct gcgggggagc gggccgtcat tctgaacaag atcgccgacc
     1801 gcatcgaggc gaacctggaa tcgctggcgc tggccgaggc gtgggacaac ggcaagccga
     1861 tccgcgagac gctggccgcc gatctcccgt tggcagtgga ccacttccgg tatttcgcgg
     1921 gcgccatccg cgcgcaggag ggttcgctga gccagatcga cgacgacacc gtcgcctacc
     1981 acttccacga gccgctgggt gtggtcggcc agatcatccc gtggaatttc ccgatcctga
     2041 tggccacctg gaaactcgcg cccgcccttg cggccggaaa tgccgtggtg ctcaagcccg
     2101 ccgaacagac acccgcgtcg atcctgtacc tgatgtcgtt gatcggtgat ctgctgcctg
     2161 cgggagtatt gaacgtggtc aacgggttcg ggttcgaggc gggtaagccc ctggcgtcga
     2221 gcaaccggat cgccaagatc gcgttcaccg gcgagaccac gaccggccgg ctgatcatgc
     2281 agtacgcatc gcagaacctg atcccggtca cgttggagct cggaggtaag agcccgaaca
     2341 tcttcttctc cgatgtgatg gccgccggtg acgaattcca ggacaaagcg ctggagggat
     2401 tcacgatgtt cgccctgaat cagggcgagg tgtgcacctg cccgtcgcgc agcctgatcc
     2461 agtccgacat ctatgacgag ttcctcgaac tcgcggcgat ccgcaccaag gccgtccgcc
     2521 agggcgaccc gctggacacc gagaccatga tcggctcgca ggcctccaac gaccagctgg
     2581 aaaaggtgtt gtcctacatc gaggtcggca aagatgaggg cgcccgcgtc gtcacaggag
     2641 gcgagcgcgc gcagctcggc ggcgacctca acggcggcta ctacgttcag cccaccatct
     2701 tcgagggcaa caacaagatg cggatcttcc aggaggagat cttcggcccg gtggtggcgg
     2761 tgacgtcgtt ctccgactac gacgacgcca tctccctcgc caacgacacg ctctacggcc
     2821 tgggcgcggg cgtgtggagc cgtgacggca acgtcgccta ccgcgcgggc cgcgatatca
     2881 aggcgggccg ggtgtggacg aactgctatc acgcctaccc ggcgcatgcc gccttcggcg
     2941 gttacaagca gtccggcatc ggccgtgaga accacaagat gatgctcgac cactaccagc
     3001 agacgaagaa cctgttggtc tcctacagca acaaggccca gggatttttc taatgccaga
     3061 cgcgccgtct cgggcgctcg tcacccgcgc ggccgccgac ctgttggaga agcttcaggg
     3121 caagtacggt gcgctgatgt tccaccagtc cggcggctgc tgcgatgggt cgtcgccgat
     3181 gtgttatccc gacggtgact tcatcgtcgg cgaccgcgac atcctgctgg ccgtcctcga
     3241 cgtcggagac acgggggtgc cggtgtggat atccggcccg cagttcgagg cgtggaaaca
     3301 cacccagctg gtcatcgacg tggtgccggg ccgcggcggc gggttcagcc tggaggcccc
     3361 ggaaggcctg cgcttcctgt cgcggggccg ggccttcacc gacgacgaaa accggtcgct
     3421 ggccgacagc gctccggtca ccggtgccga gtacgagcgt ggcctgcggc ccgccgcagg
     3481 cggcacacac gtggtcgcgg aggcaattga tgcgtgtccg atacctggca cgccgcgccg
     3541 ttaagccgtt tcgactgcgg gcggcgttat cgtcgaaaac atgatccccg taccgcgggc
     3601 atggttgttg gccagcgcga tgctggtcgg gacggcggtc gg
//
LOCUS       DASYLX010000201         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1873758,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000201 DASYLX010000000
VERSION     DASYLX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873758"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..107)
                     /locus_tag="VGP27_04800"
                     /pseudo
     CDS             complement(1..107)
                     /locus_tag="VGP27_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
     gene            1001..1411
                     /locus_tag="VGP27_04805"
     CDS             1001..1411
                     /locus_tag="VGP27_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850672.1"
                     /translation="MSFKSFKKTVARSAIVGALGFSGLGLGVGLGTASADHGNGGQQC
                     WPQNCQGDQRGDGGQDHRGDGGDGRQFGDQWRPDQWRPDRGGWDQRPWDQRGFDDARW
                     DHQPFNWQGQRVEPYFDNDRGAWGFWFLGLWIPL"
     gene            complement(2138..3076)
                     /locus_tag="VGP27_04810"
     CDS             complement(2138..3076)
                     /locus_tag="VGP27_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7850673.1"
                     /translation="MTTIWVVSFLIAGCTSDGGRTDGATGDFARAIGIGGRDVYLTCH
                     GRAAAGAPTIVLISGYHDSSDVWTQADVLGLLPPAVGPPVFPALAESHRVCAYDRPGT
                     LRYVEGTPLTNRSTPVPQPRTAGELVAELRDVLAAAGIAPPYVVVGHSLGGLIARAYA
                     QAHADQVRGVVFVDAFSATIPAVFASRWPTYRDEGLNPPIGRMPIASMRNPDSERVDL
                     DASVAEVLAGPPFPKIPVAVLTKTEPFAGLTSFPGLPADETNRLYKQAQDAIVALVPN
                     TPHLIATGSDHYIQFSQPDLVVRATELVVERAVAQP"
     gene            complement(3186..>3420)
                     /locus_tag="VGP27_04815"
     CDS             complement(3186..>3420)
                     /locus_tag="VGP27_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEV7850674.1"
                     /translation="WTIHQGDTAPKAAGVIHTDFEKGFIKAEIVSYDDLIGAGSMAAA
                     KAAGKVRMEGKDYVMADGDVVEFRFGSASTSKQ"
BASE COUNT          644 a         1055 c         1115 g          606 t
ORIGIN      
        1 aacgcgatga tgatggcgtc gtctgcgtac ccaattacgg ggatgaagtc cgggatgaga
       61 tctatcggta tggcgaggta accgagcaac accgccaacc ggacccgaac tcctcgaggc
      121 atgcccggat cggtggcgag ccgcttgatt aggcgtacca ggtcggggag aaggcgcgcc
      181 gaatctcgga ttccgaggtc gtcgggcttt ccgatccaaa gcatgaccat gagcgttatc
      241 catagcagga cgacaccgcc ggcaatgctg agcggtatcg accaccagtg ggaccacata
      301 gctcaatact tgcccggcaa tcgatgaaag gccgctcttg tgcgtcggca cgccatccaa
      361 gatccccacg ggtagcgggc cgatttctgt aaccagacgc acgcgaaatc tagtcggcgc
      421 gcctgtcgaa gtagccccgc gcccaactat ccgggggcgc ctgacggtca aacatgtcgt
      481 ggtgaggact aagcagctgg atcgaagtga ggcacatcag cgacgctgtt cattgccaga
      541 tcgacgggga cggctgcaga gggttggtca caggccgcac acagaccagc acttgggtat
      601 ccgcacgccg aacaaatcgt tacgcgccgc ctaccgtctg taccggcatg gtggccatcg
      661 gggttcctcg ctgttgccag agtttgtatc cgccgcaaag gctgttcacg caaacctgcc
      721 tagccgggag gaagttctag cactccgacg tcgggactgt ggtgagtgat ccggcccact
      781 acgggtttgg gtaacagccc tgggggacac ctcgatagag caagtgaggg tctcactgtc
      841 gcacggtata gcagtgcgac gcatcctcga acgagcatcg ccattcctcg aagcgacgcc
      901 gcctgccaag cgaaagcgta aggcggtcgc gagcggtgcc ggctttcgga tctgtcgaca
      961 cgattcggag gcacgctcac aacactgaag gagcacaaca atgagtttca agagtttcaa
     1021 gaagactgtg gcgcgatccg cgatcgtggg cgcgttgggc tttagcggtc tcgggctggg
     1081 cgtcggtctc ggcacggcgt ccgccgacca tgggaatggt gggcagcagt gctggccaca
     1141 aaactgccag ggcgaccaac gtggtgacgg tggacaggat caccgtggag acggtggcga
     1201 cggtcgccag tttggggacc agtggcgacc ggaccaatgg cgaccggatc ggggtggctg
     1261 ggaccagcgg ccttgggatc agcgcggctt cgacgacgcc cgttgggacc atcagccctt
     1321 caactggcag ggtcaacgtg ttgagccata cttcgacaac gaccgcggtg cctggggatt
     1381 ctggttcctg ggcctctgga tcccgctgtg atctgagcgg gtgaacgtcc gcgcctccat
     1441 gctaacgccg cgctagcgat gggtggggcc ggtggcgata tgccgctggc cccaccgtca
     1501 cgtctgatct aacgttcccc cgttcggccc gccactctct tgtctccgat atcgacttcc
     1561 tccgcaagga aatcaaagtg gagcggcagg tccagcgagc caacggtggc gaggtggaaa
     1621 tacggccgcc gaagttcggt tccgaacgca ctatctaagc gccggaaggg cagctcgagg
     1681 tgctcgccga acacatccgc ctacacctcc cggagcagga gtccgacagt cgtgcttctg
     1741 ctccgaggtg gttgtttccc cgcgagggtg atcacccgct gcaccagaac tcggtggcct
     1801 attggtggcg cgggcgaagg ccgctgcggg gggtcgagta ccgcctgcat gattgccgtc
     1861 acttctacgc ctcgggtctc atcgcctagg gctgtgatgt agtgactgtc cagcgtgcgc
     1921 ttgggcactc ctcccctgct gtcacgttga acacttacgc acacctctgg cccgatgcga
     1981 atgaccgcac acggaaggct gccggggagc tgttcgacgc gtccctgaaa gctgctgcgg
     2041 acccactgcg gaacgcgagc caaaatacct gccgaacagg tttttagacg ttgaaccgga
     2101 actcgaccac atcgccgtcg gcatgagagc tgacccttca gggctgcgcg acggcgcgtt
     2161 ccacgaccag ttcggtggcg cgtaccacca agtccggctg cgagaactgg atgtagtggt
     2221 cgctgccggt ggcgatcaga tgtggcgtgt tcggtaccag tgcgacgatg gcgtcctggg
     2281 cctgcttgta gagtcggttg gtttcgtcgg cgggcagtcc cggaaacgac gtcaacccgg
     2341 cgaacggctc cgtcttggtc aacaccgcca ccggtatctt cggaaagggt ggtcccgcaa
     2401 gcacttcggc gacgctcgcg tcgaggtcaa cccgttcgga gtcagggttg cgcatcgagg
     2461 cgatcggcat tcggccgatg ggagggttga ggccctcgtc gcgataggtc ggccagcgtg
     2521 aggcgaagac cgcggggata gtggcgctga aagcgtcgac gaacacgacg ccgcgtacct
     2581 gatcggcgtg ggcctgcgca tacgcgcgag cgatcagtcc gccgagtgaa tgaccgacca
     2641 cgacatacgg cggcgcgatc cccgcggcgg ccagcacgtc acggagttcc gcgacgagtt
     2701 cgccggcggt ccgcggctgc gggaccgggg tgctgcgatt ggtgagcggt gtgccctcga
     2761 cgtaccgcag cgtgcccgga cggtcgtacg cgcagacccg atgtgattcg gcaagtgcgg
     2821 gaaagaccgg tgggcccacg gctggaggca gcagaccgag cacgtcggcc tgcgtccaga
     2881 cgtccgaaga gtcgtggtag ccagagatca agacgatggt tggcgcaccg gccgcagccc
     2941 gaccatgaca cgtcaggtag acatcccgtc caccgatccc tattgcgcga gcgaagtctc
     3001 cagttgcgcc gtcggttcgg ccgccatcag acgtgcaccc agcgatgaga aacgacacca
     3061 cccaaatcgt cgtcactaaa cacttcgtcg cccggccgca tcctcgcatc agcgcgaagg
     3121 tcccaggtgc gtaccccagt cgcgaccgcg gtagcgaatc atcgcacctg accacctcgg
     3181 tgctgctatt gcttgctcgt agacgcagat ccgaaccgga attcgactac gtcgccgtcg
     3241 gccatcacat agtccttgcc ttccatccgg accttgccgg cggccttggc cgcggccatc
     3301 gagccggcgc cgatcaggtc gtcgtaggag acgatctcgg ccttgatgaa gcccttctcg
     3361 aagtcggtgt ggatgacccc ggcggccttc ggcgccgtgt cgccctgatg gatcgtccac
//
LOCUS       DASYLX010000202         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3080610,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000202 DASYLX010000000
VERSION     DASYLX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080610"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGP27_04820"
     CDS             <1..303
                     /locus_tag="VGP27_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEV7850675.1"
                     /translation="YAKRTAIDEYPVQGRGGKGVLTIQFDKRRGSLVGALVVDDETEL
                     YAITSGGGVIRTTARQVRKAGRQTKGVRLMNLGEGATLIAIARNAEESDVETEAAE"
     gene            338..1180
                     /locus_tag="VGP27_04825"
     CDS             338..1180
                     /locus_tag="VGP27_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEV7850676.1"
                     /translation="MTSPNDAGHPRSGDGSGNGSIPDDSETGSLGNAIGRDVPPWQRG
                     PAARPAQKPPVRTPREPARPEPPRGGQPGHPPGADARLNRFISGGSAPPETVEQPHPR
                     SEPVRGEPVIGEAYASELPDLSGSPRPTPRKTAPERSEPPPRPATPGRVQVAGRTHTG
                     PVRASMQIRRIDPWSALKVSLVLSVALFFVWMIAVAFLYLVLGGMGVWSKLNSNVGDL
                     LTSASGQAGGELVSSGTIFGGAALIGLVNIVLLTAMATVGVFIYNLTTDLVGGIEVTL
                     ADRD"
     gene            1242..1315
                     /locus_tag="VGP27_04830"
     tRNA            1242..1315
                     /locus_tag="VGP27_04830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1276..1278,aa:Ile,seq:gat)
     gene            1438..1510
                     /locus_tag="VGP27_04835"
     tRNA            1438..1510
                     /locus_tag="VGP27_04835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1471..1473,aa:Ala,seq:tgc)
     gene            complement(1515..2054)
                     /locus_tag="VGP27_04840"
     CDS             complement(1515..2054)
                     /locus_tag="VGP27_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7850677.1"
                     /translation="MYGDTINGIPRVDLQARPLWKRNLSWAMGGPLFASKKGLALWAR
                     VAAPLEAPLMKATGGRVRLAFSIPIVVLTSVGARSGQRRDNPLAYFTDGDDVVLIASN
                     SGGARHPAWYHNLLAHPECELHIGQKGGSFMAREVEGPERDRLYVLAVERLAKVFALH
                     EKRCGSRTIPVMRLTPEMQ"
     gene            complement(2155..2571)
                     /gene="cwsA"
                     /locus_tag="VGP27_04845"
     CDS             complement(2155..2571)
                     /gene="cwsA"
                     /locus_tag="VGP27_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthesis protein CwsA"
                     /protein_id="HEV7850678.1"
                     /translation="MNTDARLTPGQRLSRGLRYTALGPVDLTRGTVGLSWQGAHSAAG
                     GLRQRFEKSRLAKEIEEAQETIAGEIAAAQEVLANLPQIIADARKPKRRPRPLVLAAA
                     GVALIAVGGVTFSIVRRSMQPEPSPRPPSVEVTPKP"
BASE COUNT          449 a          837 c          864 g          422 t
ORIGIN      
        1 tatgcgaaac gcaccgcgat cgacgagtac ccggttcagg gccgcggtgg caaaggggtg
       61 ctgaccatcc agttcgacaa gcgacgtggc agtcttgttg gagcgctggt ggtcgatgac
      121 gaaaccgagc tctacgccat cacgtcgggc gggggcgtca tccgaacaac tgcgcgccag
      181 gttcgcaagg ccggacggca gaccaagggc gttcggttga tgaacctggg cgagggcgcg
      241 acactgattg cgatcgctcg caacgcagag gaaagcgacg tcgagaccga agccgctgag
      301 tagggcccgg cgacgtgacc gacggtaagg agccggagtg acttcaccga acgacgcggg
      361 ccatccgcgc tcgggagacg gtagcggcaa cggttccatt cccgacgact ccgagaccgg
      421 ctcactgggc aacgcaatcg gccgcgacgt gccaccgtgg caacgcggcc ccgccgcacg
      481 gcctgcgcag aagccgccgg tccgcacgcc tcgtgagccg gcccgcccgg aaccgccaag
      541 gggcggccag cccggccacc ccccgggcgc agacgcccgc ctcaaccggt tcatctccgg
      601 tgggtcggcg ccgccagaga cagtggagca accacacccg cgaagcgaac cggtccgcgg
      661 cgagccggtg atcggcgagg cgtacgcaag tgagctgccg gacctgtccg gctcaccgcg
      721 accgacaccg cgcaagaccg cacccgagcg cagcgagcca ccgccgcgtc ccgcgactcc
      781 cggccgcgtc caggtggcag gccgcacgca tacggggccg gtgcgggcca gcatgcagat
      841 ccgccggatc gacccgtgga gcgcgctgaa ggtctcgctg gtgttgtcgg tcgcattgtt
      901 cttcgtctgg atgatcgcgg tcgcgttcct gtacctggtg ctcggcggca tgggcgtgtg
      961 gagcaagctc aacagcaatg tcggcgacct gctcaccagc gcaagcggtc aggccggcgg
     1021 cgaactcgtg tccagcggga cgatcttcgg cggtgcggcg ctgatcggcc tggtcaacat
     1081 cgtgctgctg acggcgatgg ccaccgtcgg ggtgttcatc tacaacctga ccaccgacct
     1141 ggtcggcggt atcgaggtga cgttggccga ccgcgactga ttccggtttg ggtcgggggt
     1201 cgcagttacg gtaatctcac cgctcggcca tgtgcgttta cgggcctata gctcaggcgg
     1261 ttagagcgct tcgctgataa cgaagaggtc ggaggttcga gtcctcctag gcccacggaa
     1321 agggtcacca tgaggctggt gttactggtc gcggcggtgg cgttggcggt tttcgtggtg
     1381 cggtcgcgac acggcgtgga ggtgtggcac gcagctgccg atcaaccttc ctgaaacggg
     1441 gccttagctc agttggtaga gcgctgcctt tgcaaggcag aagtcagggg ttcgaatccc
     1501 ctaggctcca caattcactg catctccggc gtcagccgca tgaccggaat cgttcgggag
     1561 ccgcagcgct tctcgtgcag ggcgaacacc ttcgcgagac gttcgacagc gagcacatac
     1621 aaccggtcac gttccgggcc ttcaacttca cgggccatga aggagccgcc cttctgcccg
     1681 atgtgcaatt cacactcggg atgggcgagc agattgtgat accacgcggg gtgtcgcgcg
     1741 ccgccggaat tcgacgcgat caagaccacg tcgtcaccgt cggtgaagta tgcgagcgga
     1801 ttatcgcgac gttgtccgct gcgcgcgccg accgacgtca acacgacaat gggaatcgag
     1861 aatgcgagtc ggacgcgacc gcctgtggcc ttcatgagcg gagcttccaa gggcgccgcg
     1921 acgcgggccc ataaggcgag ccctttcttg cttgcaaaca gcgggccgcc catcgcccag
     1981 ctgagattgc gcttccacag cggacgcgct tgcaggtcga cgcgagggat tccgttgatc
     2041 gtatcgccgt acattatgag cccaccttca cgccgaaacc tgcgcgaagg tcgtaattcg
     2101 acagaatcga cgaccgcctt gcagaaatcg cggacggtac gtcaggttcg accgctatgg
     2161 cttcggtgtc acctctacgc tcggcggccg cggtgacggc tcgggctgca tcgacctgcg
     2221 gacaatcgaa aacgtcactc ccccaacggc aatcagcgcc acgcccgcgg ctgcaagtac
     2281 caatggccgg ggccggcgct ttggcttgcg cgcgtcggcg atgatctgcg gaaggttggc
     2341 gaggacttcc tgcgccgcgg cgatttcacc ggcgatggtc tcctgcgcct cctcgatctc
     2401 cttggccagc ctgctcttct cgaagcgctg acgcaggccg cccgccgcgg agtgtgcccc
     2461 ctgccagctc agtccgacgg tgcctcgcgt caggtcgacc ggaccgagcg cggtgtagcg
     2521 caggccgcgc gacaatcgct gtccgggggt cagccgggca tccgtgttca ac
//
LOCUS       DASYLX010000203         4925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1983977,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000203 DASYLX010000000
VERSION     DASYLX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983977"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..735)
                     /locus_tag="VGP27_04850"
     CDS             complement(304..735)
                     /locus_tag="VGP27_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850679.1"
                     /translation="MWDEAAKWLNKFPEAVLTAIDRNGYPVSYRVSTSAYNKASGELP
                     VSPPPGPIDVADGPANLLCHSHDEKLWNLQMITVKGALERRDGTWFFRTESFKPPSKL
                     AVLDFVRNLRRSAQKYLDKRRLERPDVNWQAIDQIKRRAKR"
     gene            complement(725..1234)
                     /locus_tag="VGP27_04855"
     CDS             complement(725..1234)
                     /locus_tag="VGP27_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7850680.1"
                     /translation="MSPSDAVDIIDRYFTCEFTTLSRNGSPITWPVSPRLLADGRFLL
                     TTSIGMPQKAFNIRRNPKVSMLFSEPTGSGIAQPGAVLIQGDATAEDRIVTDVSSDPD
                     LAALAETLTARQPAGAFWSTRVGRRLGWPYYMRLLIFVTPRRVLYWPTRDFSTTPEEL
                     DANEARRVG"
     gene            complement(1293..1610)
                     /locus_tag="VGP27_04860"
     CDS             complement(1293..1610)
                     /locus_tag="VGP27_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7850681.1"
                     /translation="MRRILGSAVWAAATVTIAAACSFSASTTPTVDKGELAKEISAQL
                     QKQVGRAPESVDCPDDLKGEVGATTRCSLNDAGETYGVNVDVTKVDGTDVKFDLKVDD
                     KPQ"
     gene            complement(1681..2886)
                     /locus_tag="VGP27_04865"
     CDS             complement(1681..2886)
                     /locus_tag="VGP27_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850682.1"
                     /translation="MTAETTGTMRRWSMLAIALTSTLFANVFINGAAFLIPTLHTERG
                     LDLARAGLLSSMPSFGMVFTLIAWGYVVDRIGERIVLALGSALTAAAAFAAASAHSLF
                     GVGTFLLLGGMAAASSNSASGRLVVGWFPPQQRGLVMGIRQTAQPLGVGLGALVIPRL
                     AENYGVSAALLFPAVVCAASAVVCAVAVKDPPRPPRTEATEDDLANPYRGSATLWRIH
                     AVSVLLVAPQVVVWTFTLVWLMTERGWSAGSAGVLVTVAQVLGAIGRIAAGRWSDVVG
                     SRLRPIRVIALAAAASMGLLALTDWLASPVSVAMVIVASVITVSDNGLAFTAIAEIAG
                     PFWSGRALGTQNTSQLLTAGVAPPLFGALIGVAGYAAAFAVCAFFPLIAIPLVPVETD
                     PLISGRTSS"
     gene            3001..3810
                     /locus_tag="VGP27_04870"
     CDS             3001..3810
                     /locus_tag="VGP27_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7850683.1"
                     /translation="MRLGRIASPDGVAFVSIEGAPDDPAAVVKEIAEHPFGSPTFTGR
                     QWPLADVRLLAPILASKVVCIGKNYAAHIAEMADAGSNAGIRGEFPDPVIFMKPNTAI
                     IGPNIPIQLPADAHPVHFEGELAAVIGRPCKDVPAARVAENILGYTIANDVTARDQQK
                     KDGQWTRAKGHDTFCPVGPWIATDVDPSDLAIRTEVNGEVKQDSRTSNMIHDVGAIVE
                     WISAVMTLLPGDLILTGTPDGVGPIENDDTVSITIEGIGTLTNPVVRKAKL"
     gene            3834..>4925
                     /gene="gltX"
                     /locus_tag="VGP27_04875"
     CDS             3834..>4925
                     /gene="gltX"
                     /locus_tag="VGP27_04875"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV7850684.1"
                     /translation="MTAHARSGNNGERVRVRFCPSPTGTPHVGLVRTALFNWAYARHN
                     GGDFVFRLEDTDPERDSEESYRALLDALNWLGLNYDEGPEVGGPYGPYRQSERRDIYR
                     DVVERLVAAGEVYEAYSTPEEVEARHVAAGRNPKLGYDNFDRTLTDEQRAAHREEGRR
                     PVLRLRMPDDAITWNDLVRGETTFAPGTVPDYALTRASGDPLYTLVNPVDDALMKITH
                     VLRGDDLLSSTPRQIALYRALMRIGVADRIPEFGHLPAVLGEGTKKLSKRDPQSNLFL
                     HRDRGLIPEGLLNYLALLGWSIADDRDVFSLDEMVAAFDVVDVNSSPARFDQKKADAL
                     NADHIRLLTEDEFTRRLRAYFDTHAYDTGL"
BASE COUNT          884 a         1626 c         1624 g          791 t
ORIGIN      
        1 cctcgccttc ggtgtctcgg cactcggcgc attcgtcacc gagacgggag tgacggagga
       61 cgcgctgctg gccaacgcgg gcaccttcgc cggtgcgctg tgcttcctgg ccgccgcgct
      121 cctggtgctg ccgcggcgcg accgcgtcga gcgtggggga taggtagagg aatcggtcga
      181 tcccgtccag atggcctacg ttcggtgtag gcaacactcg ggtgcaccaa ttccgcagcg
      241 gctcgtcgtc cagttgttcg gtgctcgcac actcgacgca taccgtcgtc ggctgttcgg
      301 gaactagcgc ttggctctgc gtttgatctg gtcgatcgct tgccaattga cgtcggggcg
      361 ctcgagtcgg cgcttgtcga gatatttctg cgcggaccgc cgcaggttcc tgacgaagtc
      421 cagcacagcc aacttcgatg gcggcttgaa gctttctgtc cggaagaacc atgtcccgtc
      481 gcgcctctcg agggcccctt tgaccgtgat catctgcaga ttccacagct tctcgtcatg
      541 cgagtggcac aacagatttg ctgggccgtc ggcaacatcg atcggaccag ggggcggcga
      601 cacgggcaat tcacccgacg ccttgttgta cgcgctagtc gacacccgat aactgacggg
      661 gtagccgttg cggtcgatcg cagtgaggac cgcctcggga aacttgttga gccacttggc
      721 cgcttcatcc cacacggcgc gcctcgttgg cgtcgagttc ctcgggggtc gtggagaagt
      781 cgcgggtggg ccaatacaga acacgtcgcg gagtcacgaa gatgaggagg cgcatgtagt
      841 agggccaccc caggcgtctg ccgacccggg tgctccagaa ggcgccggcc ggttgacgcg
      901 cggtcagcgt ttcggcgagc gcggcgaggt cgggatccga cgagacgtcg gtcacgatgc
      961 ggtcctcggc cgttgcgtct ccctggatga gcacggcgcc gggttgtgcg atgccgctgc
     1021 cggtgggctc ggagaacagc atgctgactt tcggattacg ccggatgttg aatgctttct
     1081 gcggcatgcc gatgctcgtc gtcagcagaa aacggccgtc ggcgagcagt cgcggcgaca
     1141 ccggccacgt gatcggtgaa ccgttgcgcg acagcgtggt gaactcgcat gtgaagtagc
     1201 ggtcgatgat gtcgaccgca tcggaaggcg acacgagcgg agcatatgcc cggcgcggac
     1261 gtgcattcgc cgatccgcag tttccgtgcc ggctattgcg gtttgtcgtc gaccttgagg
     1321 tcgaacttga cgtcggtgcc gtcgaccttg gtcacgtcga cgttgacgcc gtaggtctcg
     1381 ccggcgtcgt tgagtgagca gcgggtcgtc gcaccgactt cgcccttgag gtcgtcgggg
     1441 cagtcgacgg actcgggcgc acgcccgacc tgcttctgga gttgggccga gatctctttc
     1501 gcgagttcac ccttgtccac ggtcggcgtt gtcgaagcgg agaacgaaca tgccgccgcg
     1561 atggtgaccg tcgcagctgc ccacaccgcg gatccgagta ttcgccgcat cgggaacctc
     1621 ctgtgtgtgc ggttgcgcag caactctggc acacaccgcg gggccctgcc ccgctttcga
     1681 tcatgatgac gtgcgaccgc tgatcagcgg gtccgtttcg acggggacga gcggtatcgc
     1741 gatcagcggg aagaaggcgc agacggcaaa cgccgccgca tagcccgcaa cgccgatcaa
     1801 cgcaccgaac aacggcggcg caacgcccgc ggtcagcagc tggctggtgt tctgtgtgcc
     1861 gagcgcccgt ccgctccaga agggccctgc gatctcggcg atcgcggtga acgctaatcc
     1921 gttgtcggac acggtgatca ccgaggcgac gatcaccatc gcgacactga ccggtgaggc
     1981 cagccaatcg gtgagcgcca gcagacccat cgaggcggcc gccgcgagcg cgatgacacg
     2041 gatcggccgc agccgcgacc ccacgacgtc cgaccaccgg cccgcggcga tacggccgat
     2101 ggcaccgagc acttgggcga ccgtcaccag caccccggcc gatcccgccg accaaccgcg
     2161 ctctgtcatc aaccacacca gcgtgaaagt ccacaccacc acctggggtg ccaccagaag
     2221 caccgacacg gcgtggatgc gccacagcgt cgcagagccg cggtaggggt tggcgaggtc
     2281 gtcctccgta gcctcggtgc gcggcggacg cggcggatcc ttcaccgcga cagcgcacac
     2341 gacggccgac gccgcgcaca ccacggcggg gaagagcagc gccgccgaga ccccgtagtt
     2401 ctccgccaat cgcggaatca ccaacgcgcc cagaccgact ccgagtggct gagccgtctg
     2461 ccggatgccc atgacgagcc cgcgttgttg cggcgggaac catcccacca ccaaccggcc
     2521 actggcggag ttgctgctcg ccgccgccat tccgccgagc aacaaaaacg tgccgacacc
     2581 gaacagtgag tgcgcagaag ccgccgcgaa cgccgcggcc gccgtcagcg ccgaccccaa
     2641 cgccagcacg atgcgttcgc cgatgcggtc gacgacgtac ccccacgcga tgagggtgaa
     2701 caccatgccg aaactcggca tcgacgacag cagaccggcc ctggccaggt cgagtccgcg
     2761 ttccgtgtgc aaggtcggga tcagaaacgc cgcaccgttg atgaacacgt tggcgaacaa
     2821 cgtcgacgtc aacgcgatcg ccagcatcga ccagcgccgc atggtgccgg tggtctccgc
     2881 ggtcatgcgc acatcgtcgc acagccgtct cagcatattg aactgtcgtc tcatatgctg
     2941 gtacagaacc agggtggagt ggccttcgat ggccccagtg ccgcccacta ggctggcctg
     3001 atgcgcctag gccgaatcgc cagtcccgac ggggtcgcct tcgtcagcat cgagggtgcg
     3061 cccgacgacc ccgccgccgt cgtcaaagag atcgccgagc acccgttcgg cagccccacc
     3121 ttcacgggca ggcagtggcc gttggccgat gtccggctgc tggcgccgat cctggccagc
     3181 aaggtggtct gcatcggcaa gaactacgcc gctcacatcg cggagatggc tgatgcgggg
     3241 agcaatgcgg gaatcagggg cgagttcccc gatcccgtca tcttcatgaa gccgaacacc
     3301 gcgatcatcg gacccaacat tccgatccaa cttcccgcgg acgcccatcc cgtgcacttc
     3361 gagggagagt tggccgccgt tatcggcagg ccgtgcaagg acgtgcctgc ggcccgggtg
     3421 gccgagaaca tcctcggcta caccatcgcc aacgatgtca cggcgcgcga tcagcagaag
     3481 aaggacggcc agtggacccg cgcgaagggt cacgacacgt tctgtcccgt cggcccgtgg
     3541 atcgccacgg acgtcgatcc gtcggacctg gcgatccgga cggaggtcaa cggcgaggtg
     3601 aaacaggaca gccgaacatc gaacatgatt cacgacgtcg gcgccatcgt ggaatggatt
     3661 tcggccgtca tgaccctgct gcccggcgac ctcatcctga cgggcacgcc tgacggggtg
     3721 gggccgatcg agaacgacga caccgtcagc atcacgatcg agggcatcgg cacactgacc
     3781 aatcccgttg tgcgcaaggc gaagttgtga ccgcggacgc gaggagcgat gttgtgaccg
     3841 cgcacgcgag gagcggaaac aatggtgaac gggtccgggt gaggttctgc ccgtcgccga
     3901 ccggcacgcc gcacgtcggc ctcgttcgca ccgcgctgtt caactgggcc tacgcgcgcc
     3961 acaacggtgg ggacttcgtg ttccggctcg aagacaccga ccccgaacgc gacagtgagg
     4021 agagttatcg agcactgctg gacgcgctga actggctggg tttgaactac gacgagggcc
     4081 ccgaggtagg cggaccctac ggcccctacc gtcagtccga gcgccgcgac atctaccgcg
     4141 atgtggtcga gcgactggtg gccgcggggg aggtgtacga ggcgtactcc acgcccgagg
     4201 aggtggaggc gcggcatgtc gcagcgggac ggaacccgaa gctgggctac gacaatttcg
     4261 atcgcacgct gaccgacgag caacgcgccg cccaccgcga ggagggccgc aggccggtgc
     4321 tgcggctgcg gatgcccgac gacgcgatca cctggaacga cctcgtgcgc ggtgaaacga
     4381 cgttcgcgcc tggcacggtg cccgattatg cgctgacccg cgcgagcgga gacccgttgt
     4441 acacgttggt caatccggtc gacgacgcgc tgatgaagat cacccacgtg ttgcgcggag
     4501 acgacctgct gtcgtccaca ccgcgccaga tcgcgctgta ccgggcgctg atgcgcatcg
     4561 gtgtcgccga tcgcattccg gagttcggac atctgcccgc cgtactggga gagggcacca
     4621 agaagctgtc caagcgtgac ccgcagtcca acctcttcct gcaccgcgac cgcggcttga
     4681 tcccggaggg tctgctgaat tatctggcgc tgctgggctg gtcgatcgcc gacgaccgcg
     4741 acgtgttcag cctcgacgag atggtcgccg ccttcgatgt ggtggacgtc aactccagcc
     4801 cggcccgctt cgaccagaag aaggccgacg cgctgaacgc cgatcacatc cggctgctga
     4861 ccgaggacga gttcacccga cggttgcggg cgtacttcga cacccacgca tacgacacag
     4921 ggctg
//
LOCUS       DASYLX010000204         7611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2313195,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000204 DASYLX010000000
VERSION     DASYLX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313195"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="ruvA"
                     /locus_tag="VGP27_04880"
     CDS             complement(<1..279)
                     /gene="ruvA"
                     /locus_tag="VGP27_04880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV7850685.1"
                     /translation="MIASIRGEVLDVALDHAVIEASGVGYRINATPSTLSALRRGTEA
                     RLVTAMIVREDSMTLYGFADADARDLFTTLLSVSGVGPKIALATLAVHD"
     gene            complement(276..830)
                     /gene="ruvC"
                     /locus_tag="VGP27_04885"
     CDS             complement(276..830)
                     /gene="ruvC"
                     /locus_tag="VGP27_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV7850686.1"
                     /translation="MRVMGVDPGLTRCGLSVIDGGTGRQVIALDVDVVRTPSDAPLHQ
                     RLLMISDAVEHWMDTHRPDVIAIERVFSQQNVSTVMGTAQAGGVVALAAAKRGINVHF
                     HTPSEVKAAVTGNGSADKAQVTAMVTRILALQTKPTPADAADALALAICHCWRAPMLA
                     RMAAAEAMAAEQKKKYRARLKAAR"
     gene            1065..2291
                     /locus_tag="VGP27_04890"
     CDS             1065..2291
                     /locus_tag="VGP27_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV7850687.1"
                     /translation="MPDETELQSATNLAVTLAWVAGAVVAAYLVGVVISWALLRIGRR
                     SGVIGDIAELTRMPLRSALVVFGSSIAVQRTSDPGSNSWRGWLDHALLILLIATITWL
                     AVSLVQVAQRRAIARYGGGDLEISDEDRQWRRIRTQVAVLRRVATALVVILGGAAILM
                     TFPRFSDIGTTVFASAGVLSVVAGLAAQTSLGAVFAGMQIAFSGAIRVGDVVQLEDGQ
                     WWGRIEEITLSYVVVRLWDERRLVLPCTYFTTEPFENWTRSATEIMGTVEFDVDFSVP
                     FDEMRAELDRLLADSDLWDGRRGVLQVTDAVGGMVRVRIVVSAPNAGALFDLRCAVRE
                     GMVSWVQHNRVLPTQRIEAAETIAAAEPRNDGDGATKRVASGLFSGSPEADARARAFD
                     HTDNRDDDYAAPNGRR"
     gene            complement(2295..3047)
                     /locus_tag="VGP27_04895"
     CDS             complement(2295..3047)
                     /locus_tag="VGP27_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7850688.1"
                     /translation="MSGHSKWATTKHKKAIIDARRGKNFAKLIKNIEVAARTGGGDPT
                     GNPTLYDAIQKAKKSSVPNDNIERARKRGAGEEAGGADWQTITYEGYGPNGVAVLVEC
                     LTDNRNRAAGEVRVAMTRNGGNMADPGSVSYLFSRKGVVTLEKNELTEDDVLTAVLEA
                     GAEEITDLGDSFEILSEPGDLVAVRTALQDAGIDYDSAEAGFQPSVTVPLDADGARKI
                     MKLVDALEDSDDVQDVFTNADIPDEILAQIDG"
     gene            complement(3129..3728)
                     /locus_tag="VGP27_04900"
     CDS             complement(3129..3728)
                     /locus_tag="VGP27_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850689.1"
                     /translation="MAKKSIPPGPRDARGVLAAKILAAAREAFAETGWAGTTIRAVAR
                     AADVDPALVYHYFGNKDGLLDAATNPPQRWLQTVAKTWATPVPELGTALLRLMLDAWA
                     DDEIGPVLRAVLQTAAHEPTTREKLRRVVETSLMGVSQLGVDERDRTVRSGLVSSQLM
                     GLAMMRYIWKIEPLASMSDDELVAAIAPNLQHYIEGEIR"
     gene            3818..5383
                     /locus_tag="VGP27_04905"
     CDS             3818..5383
                     /locus_tag="VGP27_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7850690.1"
                     /translation="MTAAQNADVIVVGAGPVGLVAACELARRGVRVRVIDKLDMPTDE
                     SRAIAVHARSLDMFDRMGVLDDLIATGVKAKGMNMFAGHKLMFRAPLDSVDSPFAFTL
                     VTAQTETERILTEHLTRLGVTVDRGLAVMTLDQDGDSVHLSLQRSDGTTEQVHTSWVI
                     GADGGHSTVRHLVGTKLHGSFKGERFILGDVEVEPHFDNTNMYTYFSPDGPVVTLPMR
                     GGRVRFLAQIHDAPGTPLNLNPQLAQLQKIVDERIGGVTITTSHWLSCFEVHHGQVPQ
                     YRWGRVFLAGDAAHIHSPAGGQGMNTGMQDAFNLCWKLATVINGEGGEALLDSYDDER
                     RPVGKRVIDFTGLLTNVATLKGIARMARDAAVRAAGHIPPAVRVMASNVEETNIAYKD
                     SPAVLASHPRHTKIAAGQYVPYIADSELQKQIGAASGVANTDHTILTITGGKPAPAAG
                     PAGRMQVLITSDETPVGGYDAVLADPDGLVAKRYGLRDGGRVVVRPDGYIGAVTELDD
                     QMGVADYFAQIRR"
     gene            complement(5858..6121)
                     /locus_tag="VGP27_04910"
     CDS             complement(5858..6121)
                     /locus_tag="VGP27_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850691.1"
                     /translation="MATQTDPRHTQNRDWRAVGQRERSHKMLITQQHAATPTASDWLG
                     TPCGPGGLQINVTETALNPNMTSAMTRQVIRWSPRAARKAVNQ"
     gene            6147..6953
                     /locus_tag="VGP27_04915"
     CDS             6147..6953
                     /locus_tag="VGP27_04915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7850692.1"
                     /translation="MAGLFGATTFTLVSIYAAVFHTTRWLAVTWIATAVTLTVLAIKL
                     AGTDPAFATSAVLLVGFIVMFVSLTGRAAIWLVDADILHENFEPLTGMFNRDGFYEKA
                     TTLLASRSRGDDRFLAVAVVNLDSFSLVGEFSGVSGSNRARVEIAARLRETARRDAVL
                     AHIADSEFVIADLFDSNDASALIERVRGTISGSPSRLSASIGVVTTPLQPLVQLPPHD
                     VLDELISIATNAMYVSRKSGGNQVTYVRGPSLTVLDDPGNDEWFDTEEPA"
     gene            complement(6964..7560)
                     /gene="pdxT"
                     /locus_tag="VGP27_04920"
     CDS             complement(6964..7560)
                     /gene="pdxT"
                     /locus_tag="VGP27_04920"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV7850693.1"
                     /translation="MSGPRVGVLALQGDTREHLAALREAGAEAMTVRRRSELDAVDGL
                     VIPGGESTTMTHLLREFELLEPLRTRLAEGMPAYGSCAGMILLASEILDAGVAGRDAA
                     PLKGIDMTVRRNAFGRQVDSFEGDIDFDGLDRPVHAVFIRAPWVERVGPDVRVLGRAA
                     DHIVAVRQDRMLATSFHPEMTGDRRVHRLFVDIVTGRA"
BASE COUNT         1231 a         2547 c         2462 g         1371 t
ORIGIN      
        1 atcgtgcacg gccagcgtgg ccagtgcgat cttcggtccc acgccggaca ccgacagcag
       61 agtggtgaac aggtcgcgcg cgtcggcgtc ggcgaagccg tagagcgtca tcgagtcctc
      121 gcgcacgatc atcgcggtca ccagccgggc ctcggttccg cggcgcagcg cggacagcgt
      181 ggacggcgtc gcgttgatgc ggtagccgac accggacgcc tcgatgacgg catggtcgag
      241 tgccacgtcg agaacttccc cgcgaattga tgcgatcatc gtgccgcctt gagtcgtgcc
      301 cggtacttct tcttctgttc ggcggccatc gcctcggcgg ccgccatccg tgccagcatc
      361 ggcgcgcgcc aacagtggca gatggcaagc gccaacgcgt ctgcggcgtc cgcgggtgtc
      421 ggcttggtct gcaacgcaag gattctggtc accatcgcgg tgacctgagc cttgtcggca
      481 ctaccgttgc cggtgacagc ggccttcacc tcactcggcg tgtggaagtg gacgttgatg
      541 ccgcgtttgg cggcagcgag cgcgacgacg ccgcccgcct gcgccgtgcc catcaccgtc
      601 gacacgttct gctgggagaa cacccgctcg atcgcgatga catccgggcg gtgggtatcc
      661 atccagtgct cgaccgcgtc gctgatcatc agcaggcgct gatgcagcgg cgcgtcggac
      721 ggtgtgcgga cgacgtcgac gtcgagcgcg atgacctggc ggcccgttcc gccgtcgatc
      781 acggaaagcc cgcaacgcgt caacccggga tcgactccca ttacgcgcac gccaaccacc
      841 ttcggtgaac acctgttcga cggcagctta gcgtggttca ccgacgcgac ccggcaagga
      901 cacgcccggc gcacacgcgc gaaagactgt tgccatgcac actttttgct tccgggcccc
      961 ccgggtatgc gtgctctccg taccccctcg atgtgacgac agcgccgagc gtcgctaacg
     1021 tcgcgaggga cctgacgtca accccgtgga gctgaactca cacgatgccc gacgagacag
     1081 aactgcagtc tgcaacaaat ctcgccgtca cactggcctg ggtcgcagga gccgtggtgg
     1141 cggcatatct ggtgggagtg gtcatttcct gggcgctgct gcgcattggc cgccgcagcg
     1201 gtgtgatcgg cgacatcgcg gaattgaccc gaatgccgct gcgatccgcc ctggtggtct
     1261 tcggctcgtc gatcgccgta cagcgcacgt cggaccccgg cagcaacagc tggcgcggct
     1321 ggctggacca cgcgctgctg atcctgttga tcgccaccat cacctggctc gccgtgagcc
     1381 ttgtgcaggt cgcccagcgt cgggcgatag ccagatacgg cggcggcgac ctagagatct
     1441 ccgacgaaga tcggcagtgg cggcgaattc gcacgcaggt cgcggtgctg cgtagggtcg
     1501 ccaccgccct tgtcgtcatc ctgggcggcg cagcgatcct gatgacgttc ccccggttct
     1561 ccgacatcgg caccacggtg ttcgcatcgg ccggcgtgct ctcggtggtc gcaggcctcg
     1621 ctgcgcagac gtcgctgggt gcggtgtttg caggcatgca gatcgccttc tccggcgcta
     1681 tccgcgtcgg cgacgtggtg caactcgagg acggccaatg gtggggccgc atcgaggaga
     1741 tcacgctgag ctatgtcgtc gtgcgcctct gggacgagcg ccggctcgtg ctgccgtgca
     1801 cctacttcac gaccgagccg ttcgagaact ggacgcgcag tgccaccgag atcatgggaa
     1861 ccgtcgaatt cgatgtcgac ttctccgtgc cgttcgatga gatgcgcgcc gaattggacc
     1921 ggctactcgc cgacagcgat ctctgggacg gccgccgcgg cgtgcttcag gtgaccgacg
     1981 ccgtcggcgg catggtgcgg gtgcggatcg tggtgagcgc ccccaacgcc ggcgcgctgt
     2041 tcgaccttcg gtgcgcggtg cgagaaggca tggtcagctg ggtgcagcac aatcgggtgc
     2101 tgccgacgca gcgcatcgag gccgcggaga cgatcgccgc ggccgaaccg cgcaacgacg
     2161 gagacggcgc gacgaagcgg gtggcgtcgg gcctgttctc cggcagcccc gaggccgatg
     2221 cgcgggcgcg cgcgttcgac cacaccgaca accgcgacga cgactacgcc gctcccaacg
     2281 gccgccgctg atggctaccc gtcgatctgc gcgaggatct cgtcggggat gtcggcgttg
     2341 gtgaagacgt cctggacgtc gtcgctgtcc tcgagcgcgt ccaccagttt catgatcttg
     2401 cgtgcgccgt cggcgtccag cggcacggtc actgatggct ggaagccggc ctcggccgag
     2461 tcgtagtcga tacccgcgtc ctgcagcgcg gtgcgcactg cgaccagatc gccgggctcg
     2521 gagagaattt cgaagctgtc acccaggtcg gtgatttctt cggcgcccgc ttcgagcacg
     2581 gcggtgagca catcgtcctc ggtcagctcg ttcttctcga gtgtcaccac acccttgcgg
     2641 gagaacaggt acgacaccga gccggggtcg gccatgttac cgccgttgcg agtcatcgca
     2701 acgcgcacct caccggcggc gcggttgcgg ttgtcggtca ggcattcgac cagcaccgcc
     2761 acgccgttgg gcccgtagcc ctcgtaggtg atggtctgcc agtcggcgcc gccggcctcc
     2821 tcacctgcgc cgcgcttgcg cgcccgctcg atgttgtcgt tgggcaccga gcttttcttg
     2881 gccttctgga tggcgtcgta gagcgtcggg ttgccggtcg ggtcaccgcc gccggtgcgc
     2941 gctgcgacct cgatgttctt gatcagcttg gcgaagttct tgccgcgccg ggcatcgatg
     3001 atcgccttct tgtgcttggt ggtggcccac ttggaatggc cgctcatggg tgtactacct
     3061 ctttcactac cgcctctttc actactgcga agtcgcgaag tccgattaat gagtctacgt
     3121 ggaccggttc atcggatctc gccctcgatg tagtgctgga ggttcggcgc gatggcggcg
     3181 acgagttcat cgtcgctcat cgaggcgagc ggttcgatct tccagatgta gcgcatcatc
     3241 gccaaaccca tgagctggga ggacaccagg ccgcttcgca ccgtccgatc gcgctcgtcg
     3301 acgccgagct gcgacacccc catcaggctt gtctcgacga ccctgcgcag cttctcgcgg
     3361 gtggtcggct cgtgggcggc ggtctgcagc acggcgcgca gcacgggccc gatttcgtcg
     3421 tcggcccatg cgtccagcat cagcctcagc agcgccgtgc cgagttcggg taccggcgtg
     3481 gcccatgtct tcgccacggt ctgtaaccat ctctgcggcg ggttcgtcgc ggcgtcgagc
     3541 aggccgtcct tgtttccgaa gtagtggtag accagcgccg ggtcgacgtc cgcggcccgc
     3601 gccacggcac ggatcgtcgt gccggcccag ccggtctcgg cgaacgcctc gcgcgccgcg
     3661 gccaggatct tggccgccag cacaccgcgt gcgtcccgcg ggccgggcgg gatggacttc
     3721 ttcgccattt tgacgcctcc gaagtttcct cttgcggtga gacttatttc atcgtatcgt
     3781 gaaactagtc tcaacgcacc gtgaatcagg agcacatatg accgccgccc agaacgccga
     3841 tgtcatagtc gtcggcgcag gccccgtcgg cctggtcgcc gcgtgcgagc tggcccgtcg
     3901 aggagtgcgt gtgcgggtca tagacaagct ggacatgccc accgacgagt cgcgtgcaat
     3961 cgccgtccat gcccgcagcc tcgacatgtt cgatcggatg ggcgtcctcg acgacttgat
     4021 cgccaccggg gtcaaggcga agggcatgaa catgttcgcc ggccacaaac tgatgttccg
     4081 cgcgccgctg gacagtgtgg acagcccgtt cgccttcacc ctcgtcacgg cacagaccga
     4141 gaccgaacgc attctgaccg agcatctgac cagacttggc gtcaccgttg accgtgggct
     4201 cgcggtcatg acgttggacc aggacggcga ctccgtgcat ctgagtttgc agcggtccga
     4261 cggaaccacc gagcaggtcc acacgtcgtg ggtcatcggc gccgatggcg gccacagcac
     4321 cgtgcgccac ctggttggca ccaaactgca cggttcgttc aaaggcgagc gcttcatcct
     4381 gggcgacgtc gaggtcgagc cacacttcga caacaccaac atgtacacct acttctcccc
     4441 cgacgggccg gtcgtcacgc tgcccatgcg cggcggacgg gtgcggttcc tggcccagat
     4501 ccacgacgca ccgggaacgc cgttgaacct gaatccccag ttggcgcagt tgcagaagat
     4561 cgtcgacgag cggatcggcg gggtcaccat cacgacgtcg cactggctgt cgtgctttga
     4621 ggttcaccat ggccaggtgc cccaataccg gtggggacgt gtgttcctgg ccggcgacgc
     4681 agcccatatt cacagcccgg ccggcggtca gggcatgaac accggcatgc aggacgcgtt
     4741 caatctgtgc tggaaactcg cgacggtgat caacggcgag ggtggcgagg ccctgctgga
     4801 cagctacgac gacgagcgac gccctgtcgg caagcgcgtc atcgacttca ccggcttgct
     4861 gaccaacgtg gccacgctca agggtattgc ccgaatggca cgcgacgcgg ccgtccgcgc
     4921 ggcgggccat attccgccag ctgtccgcgt gatggcgagc aacgtcgagg agacgaacat
     4981 cgcatacaaa gacagtcccg ccgtgctggc ctcccatccg cggcacacga aaattgctgc
     5041 aggccaatat gttccgtaca tcgccgactc agagctgcag aagcagatcg gtgcggcgtc
     5101 gggggttgcc aacaccgacc acaccatcct gaccatcacc gggggtaagc cggcacccgc
     5161 cgcaggccct gccggccgga tgcaggtgct catcacctcc gacgagactc cggtcggtgg
     5221 ttatgacgcc gtcctcgcgg atccggatgg cttggtcgcc aagcgttacg gcctgcgcga
     5281 cggtggccgc gtggtggttc ggcccgacgg ctacatcggc gccgtcaccg aactcgacga
     5341 tcagatgggg gtcgccgact atttcgcaca aatccgacgg tgagcgatct acacgtttga
     5401 catccacccg caggggatgt tggaggaccg cttcgaaacg cgcgagcgct atgccgattc
     5461 gggcgatcag gtcacgcggt gacggctgga gtcggagcct tcggccaagt cggcaattcg
     5521 ccgatgccgt ggatgaatgg cgccgacgac tacttcgtgt ccagccgaac acccccgtct
     5581 tcgacggcga gtacacctga aacccggcac cccgccggtg ccgccacgac cggtgagccg
     5641 aggtcttggc cgatgatgct cgattggcgg gaacgtcaat tcgcgcagaa tagatcgctc
     5701 agcaaattgt caggctagaa cgagatttcg cgcgaatata tcgcgcagga cgcgatgaca
     5761 cgtttgctga attgctactc tgcctaaatg caggcgcagt gccatatgag tcgccgatga
     5821 atgcggcgca acgatggtgg cgcggccttg atcactacta ctggttgacc gcttttctcg
     5881 ccgcgcgcgg actccagcgg attacctgcc gggtcatcgc gctggtcatg ttggggttga
     5941 gcgccgtttc ggtgacgttg atttggagcc cgcccggccc gcacggcgtg cccagccagt
     6001 cgctcgcggt cggggtggcc gcgtgctgtt gggtaatgag cattttgtgg ctgcgctccc
     6061 gctggcctac cgcgcgccaa tcccggtttt gtgtgtgcct cggatcggtc tgcgtcgcca
     6121 cttcatgtct gattcaatcc gatccggtgg ccgggctgtt cggtgcgaca acgttcacgc
     6181 tcgtatccat ctacgccgcg gtatttcaca ccaccagatg gctggccgtc acctggatag
     6241 cgaccgccgt gacgctgacc gtgctggcga tcaagctggc cggaaccgac ccggcgttcg
     6301 caacctccgc cgtgctgttg gtcggcttca tcgtcatgtt cgtctcgctc accggccggg
     6361 ccgcgatctg gctcgtcgac gccgacattc tgcacgagaa cttcgagcca ctcacaggga
     6421 tgttcaaccg cgacggcttc tacgaaaagg ccaccacact gctggcatcg cgcagccgcg
     6481 gcgacgaccg gttcctcgcc gtcgcggtgg tcaacctcga cagcttttcc ctcgtcgggg
     6541 aattctccgg ggtgagcgga tccaaccgcg cccgcgtcga gatcgccgcc cgcctgcggg
     6601 agaccgccag acgcgacgcg gtgctcgcgc acatcgccga ctccgaattc gttatcgccg
     6661 acctgttcga cagcaacgac gcgtcggcgc tgatcgagcg ggttcgcggc acgatctcgg
     6721 gctcaccttc ccgcctgtcg gccagcatcg gtgtggtgac gacacctctg cagccactcg
     6781 tgcagctgcc tccgcacgac gtgctcgacg aactcatatc gatcgccacc aacgcgatgt
     6841 acgtatcacg caagagcggc ggcaatcagg tcacctacgt tcgtggtccg tcgctcaccg
     6901 tgctcgacga tcccggcaac gacgaatggt tcgacaccga ggagccggcc tagcgctcac
     6961 ccgtcacgcg cggccggtga cgatatcgac gaagagccgg tggacgcggc ggtcgccggt
     7021 catctcgggg tgaaacgacg tcgccagcat cctgtcctgg cggaccgcga cgatgtggtc
     7081 ggcggctcgg ccgagtaccc gcacgtcggg accgactcgt tccacccacg gtgccctgat
     7141 gaacaccgcg tgcaccggtc ggtcaaggcc gtcgaagtcg atgtcgccct cgaaggagtc
     7201 gacctggcga ccaaaggcat tccgccgcac ggtcatatcg atccccttaa gcggagcggc
     7261 atcgcgtccc gcaacgcctg cgtccagaat ttcgctggcc agcaggatca ttccggcgca
     7321 ggaaccgtag gccggcatgc cctcggcgag ccgggtccgc agcggctcga gtagttcgaa
     7381 ctcgcggagc agatgcgtca tcgtggtgga ctccccgccg gggatgacga gcccgtcgac
     7441 ggcgtcgagt tcgctgcggc gccggaccgt catcgcctcg gcacccgctt cgcgcagcgc
     7501 tgccaaatgc tcgcgggtgt caccctgcag cgccagcacg ccgacgcggg gcccgctcac
     7561 tgccgtgccg aggtgaaatc gcgcttgtag cgggtcaacc cctcctgcat c
//
LOCUS       DASYLX010000205         7637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_886915,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000205 DASYLX010000000
VERSION     DASYLX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886915"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGP27_04925"
     CDS             complement(<1..342)
                     /locus_tag="VGP27_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7850694.1"
                     /translation="MAAVIACRSESRPAQSSAASSHAGGFAATLRQRVTAEEMMVHLR
                     SLQHIADANDGNRASGTPGYQASVDYVAHVLQSKGFDVQTPEFELRLPFADAPIVTVG
                     GANFAAKALEYT"
     gene            682..1137
                     /locus_tag="VGP27_04930"
     CDS             682..1137
                     /locus_tag="VGP27_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV7850695.1"
                     /translation="MAFAEDMIPHHRQAVDMTAWVPSRSTNPDLLVMATHISSDQQAE
                     IRTMKGLLAQWGVQDAPGHDNSMDHMGMPVAGMVDGATLNKIQSLSGPSFDALWMTSM
                     ISHHQGAIAMAQNEIDQGRNPDAIQLAKMIISAQQREIAQMNHLMMVSE"
     gene            complement(1439..2137)
                     /locus_tag="VGP27_04935"
     CDS             complement(1439..2137)
                     /locus_tag="VGP27_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV7850696.1"
                     /translation="MRREPQNMVLLLVGVSLAFITFSGAYTRYVKPGMLPWLLVSAAL
                     LIALAACAIVRDLRAGQATDDSGHSDRGGVVWLLVLPIVVLTFVVPPALSPRAAAPTV
                     MATNTRPFPPLPTGRAPTVPLPEVLMRIATGTAGGLDDKPITISGFTMRNGEHVDLAK
                     IVIVCCAADAQLARLHLAGPAVSMAAQLPDNTWVRVEGTVPSGQRYSGTSSIPTLDVS
                     DVVRIDPPANTYGS"
     gene            complement(2134..3051)
                     /locus_tag="VGP27_04940"
     CDS             complement(2134..3051)
                     /locus_tag="VGP27_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV7850697.1"
                     /translation="MVLCAFGGAWMHQGVAGNARLATAGTVFCGVFLQAVPFLVLGVL
                     ISGLVATFVSPDRLTRWLPRRPVGAVLTAGVAGAALPGCECASVPVARRLFGEGSAGA
                     AALTFMLSSPAINPVVLVATAVAFPGQPAMVLARAVASLASAVVVGVLWSRWGRLSWI
                     TRALPRPHHSGKSRWTVFTEAARHDFLQAASYLVIGAAAAATLRVVVPPWVFEHVAGH
                     LLIGVVTMALLAFVLALCSEADAFVAASLTMVPLLPRLVFLVVGPAVDVKLVAMQAGL
                     FGRAFALRFAPLTLVVTTVVATIVGLVLL"
     gene            complement(3311..4132)
                     /locus_tag="VGP27_04945"
     CDS             complement(3311..4132)
                     /locus_tag="VGP27_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HEV7850698.1"
                     /translation="MCSSLVAVRVTVAYAVTLLLVATTLLVLGPRVQDRVVSHLSTNL
                     HNLAHGHLGTLLGSAFVTAQGEVYVLLPGLVCLLALGELLWRSGRLVLAFALGHIGAT
                     LIVAVGLAAAIKFGWLPISIARTSDVGLSYGAMAVLGTLTAAIPPRWRPAWIGWWLAV
                     ALVVVASGAEFTAAGHTVALMLGMLLSTRFRSATHWTLVRRALLAVGVAVGYLMLVGV
                     ALPTAPVAAATGILVALIAQWVARRWSSRRVHQSLSSRPEGSRAPTGIRHPALLR"
     gene            4327..5643
                     /locus_tag="VGP27_04950"
     CDS             4327..5643
                     /locus_tag="VGP27_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7850699.1"
                     /translation="MLDWSLLSGPVPVVLRLVALAIGVWLVWSTLPWRRRGWMAIAEL
                     IACLTVAALATVVLDDLARNVWMLFPDRLDLAIYLWVGVAFFAVCLAVRRIASRPRLR
                     RGVVLVTAAVTIVTACANQINAAYGAYLTPRDALGLARYDDIALRDAKGDGRLLPDVD
                     PVSSRWSAPPGLPGRGKVTSAPIPAPASGFTARIGKIYLPPAYFADPRPRLPALVLLA
                     GQPGTPQDWLSAGKLARIMDRFAADHHGLTPVVVVADDTGSRFGNPLCLDSRRGKADT
                     YLARDVPAWIRTHLTVDPDSESWAVGGVSYGGTCALQLATNHPDVYPTFLDISGSAEP
                     TLGDRDRTVAAAFGTDRAAFVRVNPLDLLRARRYSGSAAAIVVGTADVDTKPDARLVY
                     RATTEAAMDSHYTEVPGSHDWRAFSDGLARELPWLAQRMGLTDAAG"
     gene            complement(5883..6674)
                     /locus_tag="VGP27_04955"
     CDS             complement(5883..6674)
                     /locus_tag="VGP27_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850700.1"
                     /translation="MSADTQVLTRREPIAAKVPEITASFWVLKLTTTAMGEAASDYLL
                     NTVGFVGFGIGLAGFAFALLVQFRTRRYNVFAYWAAVMMVAVFGTMAADVVHHHLGVS
                     FGMSTLFCALAVAAIFWAWHRSENTLSIHSITTRRREVFYWLTVSFTFALGTAAGDLT
                     ASQLHFGLAGSIVLFAIIMAVPALGCWRFRLNSVVAFWFAYIVTRPLGASVADWVSKP
                     TKVGGLDYGDGPVAAVLLVIALILVAVAAVRRTSNTSLAPAIVTT"
BASE COUNT         1420 a         2546 c         2439 g         1232 t
ORIGIN      
        1 ggtgtactcc agcgccttgg cggcgaagtt tgcgccaccg acggtaacga tgggcgcgtc
       61 ggcgaagggc agccggagct cgaactcggg cgtttgcacg tcaaacccct tcgattgcaa
      121 gacatgagcg acgtagtcga cactggcctg atagccggga gtgccggatg cccgattgcc
      181 atcgttggcg tcggcgatgt gctgcagcga cctcaggtgc accatcatct cctcggcggt
      241 cacccgctgg cgcagcgtcg ccgcgaaacc tccggcgtga gagctcgcgg cgctggactg
      301 cgccggccgg gattcggatc gacatgcgat cacagctgcc atcgtgatca gaatgatcag
      361 cagtattcga gccgcttttg aggtgcaccg cacggggccc acggtagcgc ggctcggcgt
      421 cacgccattt cagggcgcgc ccgtcattcg ttcgactcca cggcatccga cgccgccaaa
      481 aagaggaggc gaatccggac gcatctttgc gccccttact cgcagtagcc tcgatctatg
      541 tcgccgaagg tgtgttgggc cgcggcagtg gtggtcctcg tcctggtctc ttgcggtgcc
      601 ccgtcaacga acgagcgaac cggtcccact ggcacctccc ctgcccacac ctcgcaatcg
      661 gccgagcaca acgctgctga tgtggcgttc gccgaggaca tgattccgca tcaccgccag
      721 gccgtcgata tgacggcgtg ggtgccgtca cgaagcacta accccgacct gcttgtcatg
      781 gcgacacaca tctcgtcaga tcagcaagcg gagatccgga cgatgaaggg actgctcgcg
      841 cagtggggcg tgcaggacgc tcccggccat gacaactcca tggaccatat gggaatgccc
      901 gtcgctggaa tggtggatgg tgcgacactc aataagatcc agtcactgag cggaccatcg
      961 ttcgatgcgc tgtggatgac gtcgatgatc agccaccacc aaggcgccat cgcgatggcg
     1021 cagaacgaga ttgaccaggg tcgcaatccg gacgctattc agctggcgaa gatgataatc
     1081 agtgctcagc agagggagat cgcccagatg aaccatctga tgatggtcag cgagtgaccg
     1141 ccggcatccc tgagccaacc tccccgtgct cggcattttt cgcagacggt gtcgacacaa
     1201 caagcccgac agcgcgccgt ccgcgggcga tcatccgctt ccgtcctcga acccggaagg
     1261 cgccgatgaa gctacggcgt aggtgcgcgg tggctcgcgc ttatttcact gtgtgcgcca
     1321 gaggatttcg acggctgtcg acgactgagc ggcggccaga acataacatg cagccagtcc
     1381 tgggctccac tgcctagccc aagcggccga ggcgtgcgca tcgtcgcgta gctcaagttc
     1441 aagaaccata ggtgttcgcc gggggatcga tacgcaccac gtccgacacg tcgagcgtcg
     1501 ggatcgacga cgtccccgag tacctctgcc cggaaggcac cgtgccctca actcgaaccc
     1561 aggtgttgtc gggcagttgt gcggccatgg acaccgccgg tccggccagg tggagccttg
     1621 ccagctgggc gtcggcggcg caacagacga tgacaatttt cgcaaggtcc acatgctcac
     1681 cgttgcgcat ggtgaaaccg ctaatggtga tcggcttgtc gtccaggcca ccggcggtgc
     1741 cagtggcgat gcgcatcaac acctcaggca gcggcaccgt cggcgcgcgt ccggtaggca
     1801 gcggtggaaa cggccgggtg ttggtcgcca tcaccgtcgg cgcggcagcc cgcgggctca
     1861 gagccggtgg caccacgaag gtcaaaacca cgatcggcaa caccagcagc cacacgacgc
     1921 caccgcggtc ggaatgccca ctgtcatcgg tggcttgccc ggcacgcaga tcacgcacga
     1981 tcgcgcaggc cgccagcgcg atcagcaacg cggccgatac gagcagccag ggcagcatgc
     2041 ccggcttgac atagcgcgtg taggcgcccg aaaacgtaat gaacgccaag ctcacaccga
     2101 ctagcagcag caccatgttc tggggttcgc gtctcacagc agcaccagac cgacaatcgt
     2161 cgcgaccacc gtggtgacca ccagggtgag cggggcgaag cgcagcgcaa aggcgcgtcc
     2221 gaacagaccc gcctgcatcg caacgagttt cacatccacg gccggtccca cgaccagaaa
     2281 caccaaccgg ggcagcaacg gcaccatggt gaggctggcc gccacaaacg cgtcggcctc
     2341 cgagcacagc gccagcacga acgccagcag cgccatggtg accacaccga tcagcagatg
     2401 acccgccaca tgctcgaaga cccacggcgg cacgacaact cgcagcgtgg ccgccgcggc
     2461 ggcgccgatc accagatacg aagccgcctg gaggaaatcg tggcgggcgg cctcggtgaa
     2521 cactgtccac cgggacttac cgctgtggtg cgggcgggga agcgcccggg tgatccacga
     2581 aagccggccc cagcgcgacc acagcacacc caccaccacc gcggaagcca gcgacgccac
     2641 ggcgcgggcg agcaccatcg ccggctggcc cggaaaggcg accgcggtgg cgaccaacac
     2701 caccgggttg atcgccggtg agctgagcat gaatgtcagc gccgccgccc cggccgaccc
     2761 ctcaccaaag agccgtcgtg ccacaggcac cgacgcgcac tcgcaaccgg gcagcgccgc
     2821 tccggctacc ccagcggtca gcaccgcgcc gacgggtcgc cgcggcagcc aacgcgtcaa
     2881 ccggtcgggc gacacgaacg tcgcaaccag cccacttatc aacaccccga gcaccagaaa
     2941 cggcacggcc tgcaggaaca caccgcagaa cacggtgcca gcagttgcca gccgcgcatt
     3001 gccagccacg ccctggtgca tccaggcgcc accgaacgcg cacagcacca tccccgctac
     3061 cagcacctcc gtcgacgtca ccatgcgact ccgaggctcg gcgacagtca ttgccatatg
     3121 gtcgcactgc cggctgtgcg cagctccact cgagactcag gcgagatagc gggcgcgctg
     3181 gcattcctcc gccataggcg cgcggcctac aggttgcgtt ggggatacac ctgttcgtgt
     3241 cagctgaaac cgtgtggatg gcggcgatct gctctaccgg cataggcgtc gtgcccccct
     3301 ggcttgagac ctaccgcaac agtgcagggt gccggatacc cgtcggtgcc cgactgccct
     3361 cggggcgaga actcagggat tggtgtaccc gccgactgct ccatcgtcgg gccacccact
     3421 gcgcgatcag cgcgaccagt atcccggtcg ccgcggcgac cggcgccgtc ggcagcgcca
     3481 ccccgacgag catcaggtag cccaccgcga cgccaaccgc cagcagcgcc cgtcgcacca
     3541 gagtccagtg cgtggccgaa cggaaccggg tggacagcag catgcccagc atcagggcaa
     3601 ccgtgtgccc agccgccgtg aactccgccc cggacgccac gaccaccagc gcgacggcca
     3661 gccaccagcc aatccaggcc gggcgccagc gcggtggtat cgccgcggtc agggtgccca
     3721 gcaccgccat cgcgccgtag ctgagcccga cgtcgctggt gcgggcgatc gagatcggca
     3781 gccagccgaa cttgatcgcg gcggccagcc cgacggcaac gatcagggtg gccccgatat
     3841 gaccgagcgc gaatgccagg accaaacgcc cactgcgcca gagcagctcc cccaacgcca
     3901 gcaggcacac cagtcccggc aacaagacgt agacctcacc ttgggcagtg acgaaggcac
     3961 tgcccagaag cgtgcccaga tgcccatgcg ccaggttgtg cagattggtg ctcagatgac
     4021 tgaccacgcg gtcctgcacc cgaggaccca gcaccaaaag tgtggtcgcg acgaggagca
     4081 gcgtcaccgc gtaggccacc gtgactcgca ccgcgaccag gctggagcac attcgcgaca
     4141 ccatctcgtt cgaccatacc gaccgagcaa ttacgtccgg tgtcgcgaca ggcaattccc
     4201 tacgcgttca gttccgactc gcagggttcc ggcagcagat ttcggcggac aatagaggtc
     4261 cgtccctttg cgccgtgcgc ccgggaggta cggggtctcg tctcagcgta gagaaggagg
     4321 tcggtggtgc tggattggtc gctgctgtcg gggccggtgc ccgtcgtgct gcggctggtc
     4381 gcgctcgcta tcggagtgtg gctggtgtgg tcgactttgc cgtggcgtcg tcgagggtgg
     4441 atggcgattg cggaactgat cgcctgcttg acggtggcag ccctcgcgac tgtggtgctc
     4501 gatgatcttg cacgcaatgt gtggatgctg ttccccgacc gtctggactt ggcgatctat
     4561 ctgtgggtcg gcgtcgcttt tttcgcagtc tgcctggcgg tgcgacgtat agcgtcgcgg
     4621 ccgcgactgc ggcgtggggt tgtgctggtg accgcggcgg tgacgatcgt taccgcgtgc
     4681 gccaaccaga tcaacgccgc gtacggtgcg tacctgacgc cgcgcgacgc gttgggcctg
     4741 gcccgctacg acgacatcgc actacgcgat gccaagggcg acggccggct gctacccgac
     4801 gttgacccgg tgtcgtcgcg gtggtcggcg cccccgggat tgccggggcg cggcaaggtg
     4861 acctcggcgc cgattcccgc gccggcatcc ggcttcaccg cacgcatcgg caagatctat
     4921 ctgccgcctg cctatttcgc cgatccccgg ccgcggctgc ccgcactggt gttgctggcg
     4981 ggtcagccag gaacgccgca agactggtta agcgccggga aattggcgcg catcatggac
     5041 cgattcgccg ccgatcacca cggtctgacc ccggtggtgg tggtcgccga cgacaccggt
     5101 agccggttcg gcaatcccct gtgcctggac tcgcggcgcg gcaaggccga cacctacctc
     5161 gccagggatg tgcctgcctg gataagaacg catctgaccg tggacccgga ctcggagagc
     5221 tgggcggtcg gcggcgtctc gtacggcggc acgtgcgcgc tgcaactggc caccaaccat
     5281 cccgacgtat acccgacgtt tctcgacatc tccggttccg ccgagcccac gctcggcgac
     5341 cgggaccgta cggtggccgc ggctttcggg acggatcgcg ccgcgtttgt ccgggtgaac
     5401 ccactcgatc ttcttcgcgc tcgccggtac tcgggcagcg ctgcggcgat cgtggtcggt
     5461 actgccgatg tcgataccaa acccgatgcc cgcctggtgt atcgggcgac gaccgaggcc
     5521 gcgatggaca gccactacac cgaagtgccg ggttcgcacg attggcgcgc cttttccgac
     5581 ggcctagccc gcgagctgcc gtggctggcg cagcgaatgg gcctcaccga cgcagcgggc
     5641 tagacacccg gccggggtgc gctggtcaag ctgctatcac gccgccatct tccccaaacc
     5701 cgctggtggt gtcgttcggg aacgaagagg ccctgactcg ctatccagct cgaggccttc
     5761 ttgcattgca gtccgcagtt ggttcgcagc agattccagc gacttcgtgc gtgcgacgtc
     5821 tagtgctgcc gaaagctgct ccacgtcgtc ggggaacaag tcggcgtcgt tgtacaacgg
     5881 catcaggttg tgactatcgc cggagcaagg ctcgtgttgc tggtgcgcct gaccgctgca
     5941 actgctacca ggatcagggc aatcaccaat agaaccgcgg cgacggggcc gtcgccgtag
     6001 tcaaggccgc cgaccttggt gggcttactt acccagtccg cgacggatgc gccgagcggt
     6061 cgggtcacga tgtatgcgaa ccagaaggcg accacgctgt tcagccgaaa acgccagcag
     6121 cccaaggcgg gcacggccat gatgatggcg aacagtacga tcgatccggc gagcccgaag
     6181 tgcagctggc tggcagtcag gtcgccggcg gcggttccga gggcgaaggt gaacgacact
     6241 gtcagccaat agaacacttc gcggcgtcgg gtggtgatgc tgtgtatcga cagtgtgttt
     6301 tcactgcgat gccaagccca gaagatcgcc gcgaccgcaa gggcgcagaa cagggtggac
     6361 atcccaaagg agacacccag gtggtggtgc acgacgtctg cggccatggt gccgaacacc
     6421 gcgaccatca tcaccgccgc ccagtaggca aagacgttgt agcgccgtgt ccgaaactga
     6481 acgagcagcg cgaatgcgaa gcctgctagg ccgatgccaa agcccacgaa gcccacggtg
     6541 ttgagcaggt aatccgatgc cgcctcgccc attgccgtcg tggtcagctt caaaacccag
     6601 aacgacgcag ttatttcagg gactttcgca gcgattggtt cgcgtcgcgt gagaacttgg
     6661 gtgtcggcgc tcacgatcgc ccaccaactg tcttagtaat atcaggcgtg ctcgtcgcag
     6721 acattggttg tcggttcctc tcgcttgccg atctgggtaa cgctcacgac atcggaagcg
     6781 tcggcgccac acggcctgtc gtagcggcgg gtcactgtca acatcccact ggaacaaacc
     6841 gctgccatcg ccgctgacac gactttgcgt cccccgccag ccgggtactc gcaccggtgc
     6901 tggcaggtac ggacgtattc cagtcaacct ggggcccgac ctggcgtcag cggtgatgac
     6961 tcggcgccgc ggattgggca ctctgccgtc atggaccaac aaccgatagc cggtgcgccg
     7021 cactagaagc cttcggaatg tccgcacagg ctcggggaga tgacgccatc agctgcttcg
     7081 gcctgggaaa gatcttcctc ggcgttcatt gaccggcggc gtcgttctct tgggattcgg
     7141 aatcgcctgg cagcgattac ggcgactatc gtggctttcg tcgcgggtga acgtcggcga
     7201 cgttgctgtg gggtcgctga gacgctggga ggcatctcgg aactgattcc cgcgctcccc
     7261 cgccaccgat cccctcgcta ggcccccgag gatgacgccg gctcggggcc cgccgtctcg
     7321 tattgcgccg ggcttacgac gggaccggcg agccggcgca gcagggtgac atcggcgtcg
     7381 agacagtttt ccgagacgga ggtttgagac acaatgacct gaagagaccc ctgacgggcc
     7441 agctatgagc ccgtggcctc atacgtggtc tatttcgaga cgaattgagt cgtctgattg
     7501 tcattgtccc caccggccag gacctgcctg aaatcagctg tcctggaagg gcccgacaaa
     7561 gaatcgggtt agagttcggg gtctaagtct gtttcaattc cattcgatcg ccactgtccc
     7621 atggcgaaag ccccgtg
//
LOCUS       DASYLX010000206         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2422450,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000206 DASYLX010000000
VERSION     DASYLX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422450"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VGP27_04960"
     CDS             complement(<1..1129)
                     /locus_tag="VGP27_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV7850701.1"
                     /translation="MSPLPLALDRRSADNRDLPSRTDVLAGALSQVIGGPVGRHALIG
                     RARFMTPLRVMLVIAVVFLALGYSSKAACLVTTGSGTADQKVANWQNQRAYYELCYSD
                     TVPLYTAELLNQGKFPYKSSWIETDSQGRPQKQFDGNVAVRYMEYPVITGTYQYLSMA
                     LAKTYSALSKLVPIGSIAEVVMFFNISALGLALAWFATVWATSRLAGRRIWDAALVAG
                     SPIVIFQIFTNFDALATAFATGAILAWARRRPVLAGALIGLGVAAKLYPILLLIPLAL
                     LGLRTGRLREVGKTAVAAVVAWLVVNLPVMVLFPRGWSEFFRLNTRRGDDMDSLFNVV
                     KSFTDWQGFDTGLGLWEPPKILNTVVFVLFLLCCAGVAYIVL"
     gene            complement(1189..3582)
                     /locus_tag="VGP27_04965"
     CDS             complement(1189..3582)
                     /locus_tag="VGP27_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV7850702.1"
                     /translation="MEVQRPRPPRRPVSHFDDRRTAVIPAVRDVPRPLRDPVEAVKAA
                     LDGTPPQKPPPPGPPRRPPPGGGGPGGRPPRRLPAMQQISWKWVRRSIYVTVAVLIVL
                     PMITFGMAYMIVEVPKPGDIRTNQVSTILASDGSELAKIVPPEGNRVDVDINQIPVHV
                     RDAVMAAEDRDFYSNPGFSFTGFARAAKNNVFGGDTQGGSTITQQYVKNALVGSQRSG
                     LGGIVRKAKELVISTKMSSEWSKDQVLQSYLNIIYFGRGAYGIAAASKAYFDKPVEQL
                     TVADGALLAALIQQPSALDPAIDPEGASERWNWVLDGMVEIGALSATDRTAQVFPPTV
                     PPDQAQNQNQTTGPNGLIERQVQKELLDLFDINEQTLNTEGLQVTTTIDPKAQSAAEG
                     AVTDTLDGQNPDMRSAVVSIDPKTGGIKAYYGGTDAQGFDFAQAGLPTGSSFKVFALV
                     AALEQGIGLGYQVDSSPVEVNGIKITNVEGEGCGTCNIAEALKRSLNTAYYRLMLKLK
                     NGPSDVAAAAHQAGVAESFPGVEHTLSEDGKGGPPNNGIVLGQYQSRVIDMASAYATL
                     AASGVYHRPHFVQKVVNAEGQVLFDAANEDNSGEQRIDKAVADNVTAAMQPIAAYSRG
                     HALAGGRPSAAKTGTNQLGDTDANRDAWMVGFTPSLSTAVWVGTTDGTQPLENKSGSS
                     VYGSGLPSDIWKDTMDGALEGTDKESFPKPTEIGGYAGPPAPPRPSPTPTQPSETVIQ
                     PSIEVAPGITIPFGPPTTVPNAPPGQPAPDPNAVGAPGTPVPPGVPGPGPQPPLPPP"
     gene            complement(3659..4084)
                     /locus_tag="VGP27_04970"
     CDS             complement(3659..4084)
                     /locus_tag="VGP27_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 domain-containing protein"
                     /protein_id="HEV7850703.1"
                     /translation="MRLQRQVVDYALRRRSLLAEVYSGRTGVSEVCDANPYLLRAAKF
                     HGKPSSVMCPICRKEQLTLVSWVFGDHLGAVSGSARTAEELVMLATRYDEFSVHVVEV
                     CRTCSWNHLVKSYVLGAVRPPKGSRPTRTARNGARTASE"
     gene            4196..5092
                     /locus_tag="VGP27_04975"
     CDS             4196..5092
                     /locus_tag="VGP27_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEV7850704.1"
                     /translation="MATRRTPGTRAKDSDRADTCQVLDSALGEGQLSMEEHRQRVGAA
                     TNATTLGELQSLVTDLQPSNPAHSPATRSSKLRRGPVVIAAVTGVVVAIGAAIAWAST
                     GDTGPAPDASAPTPSAEAPAPHIMTAIPTTEAQLPDEPAPVVLAPLRQLHTADGMAGL
                     IEEIRKRFGDTMGYELAISPDEAVLARPNPADDQSKLIYSFKGGWGDPDTRPRSDTDN
                     VTDLGAFDVQAADAALQGAPDTLRIAPADVSDTVIDIDHISDPAGPGALELLVKVTTT
                     SGANGFIYLDGASNVKRVEYPG"
     gene            5110..>5620
                     /locus_tag="VGP27_04980"
     CDS             5110..>5620
                     /locus_tag="VGP27_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEV7850705.1"
                     /translation="MATRQAPGTRATDSDRTDTCQVLDSAFSEGQLSMEEHRQRVASA
                     TNAATLGELGDLVADLQNANAPVQLPTLKSRPRSPSKLWALPTGGGWGLRLATGGVLV
                     LLGMGIGWGLYGNTSSPLSLQTDPGAKSDGISAKVLTPPRQLQSLGGLNGLFEQMRQK
                     FGDTKGFELN"
BASE COUNT          947 a         1897 c         1834 g          942 t
ORIGIN      
        1 tcagcacgat gtaagcgaca ccggcacaac acaataggaa cagcacgaac accactgtgt
       61 tgagaatctt cgggggctcc cacagcccca gcccggtgtc gaagccctgc cagtcggtga
      121 acgacttgac cacgttgaac agcgaatcca tgtcgtcacc gcgacgggtg ttgagccgga
      181 agaactccga ccagcctcgc gggaacagca ccatcaccgg caggttcacc accagccacg
      241 cgacgaccgc ggccaccgcc gtcttaccca cttctcgaag ccgacccgtc cgcaggccga
      301 ggagtgccaa cgggatcaac agcaggatcg gatacagctt ggccgccacg ccgagaccga
      361 tcaatgcccc ggcgagcacc ggtcttcgcc gcgcccacgc caatatggcg cctgtcgcga
      421 aagctgttgc cagagcatcg aagttggtga agatctggaa gatcacgatc ggcgaacccg
      481 cgaccagcgc cgcgtcccag atccgtctgc ccgccagtct cgacgtcgcc cacaccgttg
      541 cgaaccacgc cagcgccagg ccgagcgcgg agatattgaa gaacatcacc acttcggcga
      601 tcgacccgat gggcaccagc ttgctcaacg cggagtaggt cttcgccaac gccatcgaca
      661 gatactgata ggtgcccgtg atgaccggat actccatgta ccgcacggcg acgttgccgt
      721 cgaattgctt ctgtggcctg ccctgactgt cggtttcgat ccagctagac ttgtacggga
      781 atttgccctg gttcaacagt tccgcggtgt agagcgggac agtgtcggag tagcacagct
      841 cgtagtacgc ccgctggttc tgccaattgg caaccttctg gtcggccgtc cccgaacccg
      901 tggtcaccag gcaggcggcc ttcgacgagt aacccagcgc gaggaagacc accgcgatca
      961 ccaacatcac ccgaagcggc gtcatgaagc gcgcccggcc gatcaacgcg tgccttccga
     1021 ccggtccgcc gatgacctgc gagagcgcac ccgccaaaac gtctgtcctg ctgggcagat
     1081 cgcgattgtc ggcactgcgc ctgtcgaggg ccagcggcag cggagacacc tgggagcgga
     1141 ccggctcagc taccactccg tgctcgactt cgctgtctgc gctctcggtc acgggggcgg
     1201 aagcggcggt tgcggaccgg gtcccggtac gcccggcgga accggcgtgc ccggcgcacc
     1261 gaccgcgttc gggtcaggcg ccggctgccc cggcggagca ttcggcaccg tcgtcggcgg
     1321 accgaacggg atggtgatgc ctggcgccac ttcgatactc ggctggatca ccgtctcgga
     1381 cggctgcgtt ggcgtcggcg acggacgagg tggcgcgggc ggacccgcgt agcctccgat
     1441 ctccgtcggc ttcgggaacg actctttgtc ggtgccctcc agtgcgccgt ccatcgtgtc
     1501 tttccagatg tcggacggca ggccggaccc gtacaccgac gatcccgatt tgttctcgag
     1561 cggttgggtg ccgtcagttg tgccgaccca caccgccgtc gacagcgacg gggtgaaacc
     1621 gaccatccag gcgtcccggt tggcgtcggt gtcgccgagc tggtttgtgc ccgtcttggc
     1681 tgccgacggg cgaccgcccg ccagggcgtg tcctctggag tacgcggcga tcggctgcat
     1741 ggcggcggtg acgttgtcgg cgacggcctt gtcgatgcgc tgctctcccg agttgtcctc
     1801 gttggctgcg tcgaacagca cctgaccctc ggcgttgacg accttctgca cgaagtgcgg
     1861 acggtggtag acgcccgacg ccgcgagcgt ggcgtaggcg gaggccatgt cgatgacgcg
     1921 gctctggtac tggcccaaca cgattccgtt gttcggcggg ccgcccttac cgtcctcgga
     1981 caaggtgtgt tccacgccgg ggaagctctc ggcgacaccg gcctggtgtg cggcggcggc
     2041 gacgtcggac ggcccgttct tcagcttcag catcagccgg tagtaggcgg tgttcaacga
     2101 ccgcttcagc gcttcggcga tgttgcatgt tccgcaacct tcgccttcga cgttggtgat
     2161 cttgatgccg ttgacctcga ccggcgagct gtccacctga tatcccagac cgatgccctg
     2221 ctcaagggcc gcgaccaagg cgaagacctt gaacgacgag ccggtcggca gcccggcctg
     2281 agcgaagtcg aagccctgcg cgtccgtccc gccgtaatag gccttgatgc cgccggtctt
     2341 cgggtcgatg gacaccaccg cagagcgcat gtccggattc tggccgtcca gcgtgtcggt
     2401 caccgctcct tcggccgccg attgagcctt ggggtcgatg gtcgtggtga cctgcaggcc
     2461 ctcggtgttc agcgtctgct cgttgatgtc gaacaggtcg agcagttcct tttgcacctg
     2521 ccgctcgatc aacccattcg ggcccgtcgt ctgattctgg ttctgcgcct gatcgggcgg
     2581 caccgtcggc ggaaacactt gtgccgtgcg gtcagtcgcc gacaaggcgc cgatctccac
     2641 catgccgtcg agcacccagt tccaccgctc cgaggccccc tcgggatcga tggccggatc
     2701 gagcgcggag ggctgttgga tcagcgcagc cagcagcgcc ccgtcggcga ccgtgagctg
     2761 ctcgaccggc ttgtcgaaat acgccttcga tgcggcggcg atgccgtacg cgccgcggcc
     2821 gaaatagatg atgttcagat acgactgcag tacttggtct ttggaccact cgctggacat
     2881 tttcgtcgag atgaccagct ctttggcctt acgcacgatg ccgccgaggc ctgatcgctg
     2941 cgagccgacc aatgcgttct tcacgtactg ctgggtgatc gtcgacccgc cctgggtgtc
     3001 gccgccgaag acgttgttct tggcggcccg cgcgaagccg gtgaacgaga agcccggatt
     3061 ggagtagaag tcgcgatctt ctgcggccat cacggcgtcg cgcacatgca ccgggatctg
     3121 gttgatgtcg acgtcgaccc ggtttccttc gggcggaacg attttggcga gttcactgcc
     3181 gtcgctggct aggatcgtcg agacctggtt ggtgcggatg tcacccggct tgggcacctc
     3241 gacgatcatg taggccatgc cgaacgtgat catcggcaac acgatcagaa cggcgaccgt
     3301 cacgtagatg gagcgccgca cccacttcca gctgatctgc tgcatcgcgg gcagacggcg
     3361 agggggccgg ccgcccggcc cgccaccgcc gggaggcgga cggcggggtg gccccggcgg
     3421 gggtggcttc tgcggcggcg tcccgtcgag cgccgccttg acggcctcga ccgggtcgcg
     3481 cagcggtcgg gggacgtccc gtacagccgg gatgacggcg gtgcgtcggt cgtcgaagtg
     3541 gctgaccggc ctgcggggag gacgtgggcg ttggacttcc accgtctcgc gttcctccac
     3601 accgtcagca gcctgccccg cagggacatc tctggctgac cggttatggc gcccttcgct
     3661 attcactggc cgtccgcgca ccgttgcgcg cggtccgggt cggacgcgag cccttcggcg
     3721 gccgaaccgc gccgagcacg tacgacttca ccaggtgatt ccagctgcag gtgcggcata
     3781 cctccaccac gtgtaccgag aattcgtcgt agcgggtcgc cagcatgacc agctcctcgg
     3841 cagtccgcgc cgaacccgac accgcgccga ggtgatcgcc gaagacccat gacaccaagg
     3901 tgagctgttc cttgcggcag atcgggcaca tcaccgaact gggcttccca tggaacttcg
     3961 cggctcgcag caagtacgga ttggcgtcac agacctccga aacgccggtg cgtcccgagt
     4021 agacctccgc cagcagggac cgccgccgga gcgcgtagtc caccacctgt cgctgcaatc
     4081 gcacgatgac cagagtacgt cgggcctccc gggcatggag gcgtgacggg gtcaggcgcc
     4141 tggcgcgtcg gtatctgtct gcgaagatgc gcaccggact cttacgatca tcgacgtggc
     4201 gacgcggcgg acaccgggca cccgtgcgaa ggacagcgac cgcgccgata cctgtcaggt
     4261 actcgacagc gcgctcggcg aggggcagct ctcgatggag gagcaccggc aacgggtggg
     4321 tgcggccacc aacgccacca cgttgggcga gctgcagtcg ttggtcaccg acctgcagcc
     4381 gtcgaatccg gcgcactcgc ccgccacccg atcatcgaag ctgcgccgcg gcccggtcgt
     4441 gatcgcggcg gtgacaggtg tggtcgtggc gatcggcgcg gcgatcgcgt gggccagcac
     4501 gggcgacacc ggtcccgccc cggatgccag cgcccccaca ccgtcagccg aggcccctgc
     4561 gccccacatc atgacggcga tcccgacgac ggaagcccag cttcccgacg agccggcgcc
     4621 ggtcgtgctt gctccgcttc gacaactgca cacggcggac gggatggccg gtctgatcga
     4681 ggagatccgg aagagattcg gcgacaccat gggttatgag ctggccatct cgcccgacga
     4741 agcggtgctc gcccgtccca acccggccga cgaccaatcg aagctgatct actcgttcaa
     4801 gggtggctgg ggggatccgg ataccaggcc ccggtccgac accgacaacg tcaccgacct
     4861 cggcgcgttc gatgtgcagg cggcggacgc cgcactgcag ggcgcgcccg acaccctgcg
     4921 aatcgcaccc gccgacgtct cggacaccgt catcgacatc gaccacatct cggatccggc
     4981 cgggccggga gcactggaac tcttggtcaa ggtcaccacg acctccggcg cgaacgggtt
     5041 catctatctc gacggggcga gcaacgtcaa gcgcgtcgaa tatcccggtt gacatctacg
     5101 atcatcgacg tggcgacgcg gcaggcaccg ggcacccgtg caacagacag cgaccgaacc
     5161 gatacctgcc aggtgctcga cagcgcgttc agcgagggac aactgtcgat ggaggagcac
     5221 cgccagcggg tggcgtcggc caccaatgcc gccacgctgg gtgaattggg ggacttggtc
     5281 gccgacctgc agaacgcgaa cgccccggta caactgccta ccctcaagtc gcggccacgc
     5341 tcccctagca agctgtgggc cctgcccaca ggcggcggct ggggccttcg gctcgcgacc
     5401 ggcggcgtgc ttgtgcttct cggtatgggc atcggctggg gcttgtacgg caacaccagt
     5461 tcgccgctga gcttgcagac cgacccgggc gccaagtccg acggcatctc cgccaaggtg
     5521 ctgacgccgc ctcggcagct gcagtcgctg ggtgggctca acggattgtt cgaacagatg
     5581 cggcagaagt tcggtgacac caagggcttc gaattgaaca
//
LOCUS       DASYLX010000207         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2861511,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000207 DASYLX010000000
VERSION     DASYLX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2861511"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGP27_04985"
     CDS             complement(<1..197)
                     /locus_tag="VGP27_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEV7850706.1"
                     /translation="MNAFEGLLHGLQSAITPTNLLWLIVGCLLGTLVGILPGLGPPAT
                     ISILLPIATGFEPATGLIMMAG"
     gene            complement(235..816)
                     /locus_tag="VGP27_04990"
     CDS             complement(235..816)
                     /locus_tag="VGP27_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEV7850707.1"
                     /translation="MTDGVAQPTADDVPPPQDDADVSPPAEDGADHARDERITRLVHI
                     VLGVVVAALGGWLLYRSRTELAFRGSNGEPGPGYLPVLLTIGLIALGLLLTAVWAFGG
                     KARHGDAPTLSLHPRQMGRALLVWGALVVCTLVIEPLGFTLAGEIFVLLIMAIERIRS
                     IPLIIALLLVPPALYLLFDTLLEVQLPGGTLWY"
     gene            1007..1813
                     /locus_tag="VGP27_04995"
     CDS             1007..1813
                     /locus_tag="VGP27_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7850708.1"
                     /translation="MQKSRPESETGPAYPIASVNNALLLLLLFREQPRVRLTDACKYL
                     GVAHSTAHRLLAMLSHHGFVQQEPVTRAYIAGPALVEVGLAVVGSLNVREQARPMMEE
                     LAAETGETVHLGVLEGDQVRYVDAVESERALRVVARTGTLVPAHCTSLGKALLGQLTD
                     QQVAELYPTSAEPFSARTDKSITTQAKLLKEVSRARSRGYALNAGETEDDVGSVAVAF
                     RDFAGRPAAIAVAAPTSRLTTPRISHIGDLMIQTIARAPESPVIAQVPGD"
     gene            complement(1795..3000)
                     /locus_tag="VGP27_05000"
     CDS             complement(1795..3000)
                     /locus_tag="VGP27_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7850709.1"
                     /translation="MVNLKERSLPPLWLVGTVASIFLLQVAVTLARPVSTYRLLALGA
                     DGTTLGLTAACFAVPPMLLAVSFGRWSERHHPAILIVLGFVVAAVGAFSLVVVEAIPA
                     FALATTALGIGHMSATIGGQSIMAQAQSSLARISRFGTLTTVSALGQIVGPVLGGVII
                     GHSDDPSLSSTSSALQVAAWVFVGGLPAAAISMRTTMQKSTVRTGKPERVWGLLRRKG
                     MPAALMTSFSAKGANDLLLVYVPLLGASVGLTSSQVGILLGISSSGALLARAATPWFV
                     RRIASVNLTVVATVVAALSLLIVGLSHNLTPMMLAMSVLGFAIGLTQTTTMDWVVDLV
                     DDTSRGSALGMRVATNRVGQTFILAAAGVASQWFGVEAAFALLAFVMLTTAGAGALSA
                     RSGPQSPGT"
     gene            complement(3017..3409)
                     /locus_tag="VGP27_05005"
     CDS             complement(3017..3409)
                     /locus_tag="VGP27_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7850710.1"
                     /translation="MRIRHLGIVVQDLEQTAEFYENVLGFKRLGEARTPGHYPGKALD
                     LSDGEVNYSLLQPNPDIQRSEWTYGAMGPNHIGVTIEDTGAVVTALKERGIEVYGAEQ
                     ADPPRFFKFRDPDGVEVDVATPERSWKF"
     gene            complement(3571..>4015)
                     /locus_tag="VGP27_05010"
     CDS             complement(3571..>4015)
                     /locus_tag="VGP27_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7850711.1"
                     /translation="LLVVGGAQLFIAMASDLSGGGRALLFISGAVSVILAVLTFRYFG
                     EAFSILLFAVYIGISFIIRGVATTVAAISGAERSARGWDIFFGIMSVLAGIIVLLVPF
                     TSIVTLAIVTGAFLVALGLFEIVSAFRTRHSSAKIPVKAGMPRAA"
BASE COUNT          762 a         1303 c         1322 g          628 t
ORIGIN      
        1 ccggccatca tgatgaggcc ggtcgccggt tcgaacccgg tggcgatggg cagcaggatc
       61 gagatggtgg ccggtgggcc gagcccgggc aggattccga ccagcgtgcc cagcaggcag
      121 ccgacgatga gccagagcag gttggtgggc gtgatcgcgg actggagtcc gtgaagtagt
      181 ccctcgaacg cgttcaccgg ccggcaccca ggccgtcgaa gtcaccgaag tcgttcagta
      241 ccacagggtt cctcctggca gctgcacctc gagcagggtg tcgaacagca agtacagcgc
      301 cggcgggacc agcagcaggg cgatgatcag cgggatggag cgaatgcgct cgatcgccat
      361 gatcagcagc acgaagatct ccccggcgag cgtgaagccg agcggctcga ttaccaatgt
      421 gcagacgaca agggcgcccc acaccagcag cgctcggccc atctggcgcg gatgcaggga
      481 cagtgtcggc gcgtcgccgt ggcgtgcctt cccaccgaac gcccagaccg cggtcagcag
      541 caggccaagc gcgatcaagc cgatggtcag tagcaccgga aggtagccgg gtcccggctc
      601 gccgttcgag ccgcggaagg cgagctccgt acggctgcgg tagagcagcc agccacccaa
      661 cgccgcgacc accaccccga ggacgatgtg caccaaccgt gtgatgcgct cgtcgcgggc
      721 gtggtcggcg ccgtcctcgg cgggcggtga gacgtcggcg tcgtcctgtg gtgggggaac
      781 gtcgtccgcg gtgggctgcg cgactccgtc agtcaagggt ttcgccttcg gttggacacc
      841 ctgatccata cagacctctt cgtggatccc agttcttcaa gatggaaagc ttttctggct
      901 ataggaaaac taggaccaat gtgatcatgt gtcaagcgtc actttcaaaa gtgacgcgga
      961 gtgcgggatc ggcaaacacc tggagaaatg acggtaggct tcttctatgc agaagtcacg
     1021 accggagagc gaaaccggac cggcctatcc catagcgtcg gtcaacaatg cgcttcttct
     1081 gcttctgctg ttccgcgaac agccgagggt gcggctgacc gacgcctgca aatacctggg
     1141 ggtcgcccac tcgacggcac atcgactcct cgcaatgctg tcccaccatg gcttcgtgca
     1201 gcaggaaccg gttacccgcg cctacatcgc aggccccgca ctggtcgagg tgggccttgc
     1261 cgtcgtcggt tcgctgaacg tccgggagca ggcccgcccg atgatggagg aactcgccgc
     1321 cgagactggt gagacggtcc acctcggcgt actggagggc gaccaggtcc gctacgtcga
     1381 cgcggtggag tccgagcggg cccttcgggt cgtggccagg acagggaccc tggtgcccgc
     1441 gcactgcacg tcgttgggca aggcgctgct gggccagctg acagatcaac aggtcgcgga
     1501 gctgtacccg acgtcggccg agccgttctc cgcccgtacc gacaagtcga tcaccaccca
     1561 ggcgaaattg ctcaaggagg tctcgcgcgc caggtcccgt ggttatgcgc tgaatgcggg
     1621 tgagacggag gacgacgtcg gctcggtggc agtggcgttc cgggacttcg ccggccggcc
     1681 ggccgccatc gcagtggccg cgccgacgag tcgtcttacc acgccacgca tttcgcatat
     1741 cggcgacctg atgatccaga caattgcccg cgcgcccgaa tcccccgtga tcgctcaggt
     1801 gcccggggac tgaggaccac tgcgcgcgct cagtgcgccc gcgcccgctg tcgtcagcat
     1861 gacgaacgcc agtagcgcga aggccgcctc gacgccgaac cactgcgacg ccacgcccgc
     1921 cgccgccagg atgaacgtct gtccgacgcg gttggtcgcg acgcgcatgc ccagggcgga
     1981 gccgcggctg gtgtcgtcca ccaggtcgac gacccaatcc atcgtggtgg tttgcgtcag
     2041 gccgatggcg aatccgagta ccgacatcgc aagcatcatc ggcgtcaggt tgtggctcag
     2101 cccgacgatg agaagagaca gcgccgcgac gaccgtggcg accacggtca ggttcacgct
     2161 ggcgatccgg cggacgaacc acggtgttgc ggcgcgggcc aacagcgcgc cgctcgagct
     2221 gatgccgagg agaatgccca cctgcgacga cgtcagcccg acggaggcgc cgagcaacgg
     2281 cacatagacg aggagaaggt cgttggcccc cttcgccgag aagctcgtca tcaacgctgc
     2341 gggcatcccc ttgcggcgca acaggcccca cacccgttcc ggcttgccgg tgcgcacggt
     2401 cgacttctgc atcgtggtgc gcatcgagat cgcggcggcg ggcagtccac cgacgaacac
     2461 ccaggccgca acctggagcg ccgacgacgt cgacgacagg ctgggatcgt cactgtgtcc
     2521 gatgatgacg ccaccgagaa ccggccccac gatttgaccg agcgcggaaa ccgttgtcag
     2581 agtgccgaac cggctgattc gggcgagcga actctgcgct tgagccatga tgctctggcc
     2641 gccgatcgtc gccgacatgt ggccgatccc gagcgcggtg gtggcgagcg cgaacgcggg
     2701 gatggcctcg acgacgacga gcgagaaagc tcccaccgcg gcgacgacga agccgagcac
     2761 gatcaatatc gccgggtgat ggcgctctga ccatctgccg aagctgactg cgagcagcat
     2821 cggcggtacc gcgaagcacg ccgccgtcag gccgagggtg gtcccgtcgg caccgagcgc
     2881 cagcagccgg taggtcgata ccggccgcgc cagtgtgacc gccacctgca acaggaagat
     2941 gctggcgacg gtgccgacga gccagagcgg cggaagtgaa cgttctttga ggttgaccac
     3001 ggcccggccg tggcgatcag aacttccagc tacgctccgg cgtggccacg tcgacctcta
     3061 caccgtcggg gtcacggaac ttgaagaacc gcggcgggtc cgcctgctcg gcgccgtaga
     3121 cctcgatgcc gcgttccttc agtgcggtga ccaccgcacc ggtgtcctcg atcgtcacac
     3181 cgatgtggtt gggccccatt gccccgtagg tccactcgga gcgttggatg tccggattcg
     3241 gctgcagcag cgagtagttg acctcgccgt cgctgagatc cagcgccttt ccggggtagt
     3301 ggccgggggt gcgggcctct ccgaggcgct tgaagcccag cacgttctcg tagaactctg
     3361 ccgtctgctc gaggtcctga acgacgattc cgaggtgtcg gatgcgcact actttctccc
     3421 tcttcgtctg aagcgttgaa atcttccgta aagtagaaaa tgtttctgca aatcagactg
     3481 taggcccgga cggtggcacg cgtcaatcgg cacgcacaga aatcaccgcc gccgaatgca
     3541 gtgctcggcg gcggtgaggt aagagaagcg ctacgcggcc cttggcatgc ccgccttcac
     3601 cggtatcttg gcgctgctgt gtcgagttct gaacgccgac acgatctcga atagacccag
     3661 ggcaacaagg aacgcacccg tcacgatggc gagcgtgaca atcgacgtga acggcaccag
     3721 cagcacgatg atgccggcca ggacggacat gattccgaag aagatatccc agccgcgcgc
     3781 gctcctttcg gcaccgctga tcgccgcgac agtggtcgcg actcctctga tgatgaagct
     3841 gatcccgatg tagacggcga acagcaggat cgaaaacgcc tcgccgaagt accggaacgt
     3901 cagcaccgca aggatcaccg acaccgcgcc gctgatgaag agcagtgccc gaccgccacc
     3961 cgataaatcg gatgccatcg cgatgaacag ttgtgcgccg ccgacgacca gcaga
//
LOCUS       DASYLX010000208         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2751117,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000208 DASYLX010000000
VERSION     DASYLX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751117"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGP27_05015"
     CDS             <1..410
                     /locus_tag="VGP27_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850712.1"
                     /translation="DMVPMPLPELFRRWYGPIPPIKETRNQTGDWDAVGQSRTVVLVG
                     GGSMREQLTRFDAPHSFGYTLSEVKGPLSPLVDHIDGEWIFEPIGAGTNVTWRWTIYP
                     RSGLTAFLLPVFGKLWKGYARQSLEELSRQLVH"
     gene            complement(415..1137)
                     /locus_tag="VGP27_05020"
     CDS             complement(415..1137)
                     /locus_tag="VGP27_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEV7850713.1"
                     /translation="MIARVAAALAAASIGFAPMAAAEPPAPPAPIVPPPVQPANIGDL
                     KTEATHYYDSGAYLTDLQQAAWPAIGWLNEEAPRVNRPAVVFDIDETALSNWEAIKAN
                     DFGRVIGGSCVHLPQGPCGWQEWDLRAQSTVIPPTMDVYDTAKDLGVAIFFITGRPES
                     QRAATERNLAAMGYTGYSRLIMEPVDAHYLSAADFKAPQRQTIESEGYTIVANMGDQP
                     SDLDGGFAERTYLLPNPFYRIP"
     gene            complement(1137..>2128)
                     /locus_tag="VGP27_05025"
     CDS             complement(1137..>2128)
                     /locus_tag="VGP27_05025"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEV7850714.1"
                     /translation="CHSDEHLVTGDLHMTMPVVGGHEGAGVVVEVGEGVTDVAEGDSV
                     VLSFIPACGRCGPCARGMSNLCELGMHLLRGPQLDGTHRFHARGHNVGQMCLLGTFSE
                     YTVVPMASVVKVDRSVPLDKAALVGCGVTTGYGSMVNTGEATAGSTVVVMGVGGVGVN
                     AVQAARIVGARHIVALDPVEYKRTRATEFGATHTAATLEEAAKLVTGLTHGQKADVCV
                     VTTDVAEGAYVAQALSLVGKRGRVVMTAMAHPDEKQVNLSLVDLTLFEKQLRGSLFGS
                     SNPRIDIFRMLDLYDSGQLMLDELITREYTLSDINSGYDDLRSGLNLRGLIRF"
BASE COUNT          350 a          702 c          708 g          368 t
ORIGIN      
        1 gcgacatggt cccgatgccg ctgcccgagc tgttccggcg ttggtacggc ccgatcccgc
       61 cgatcaagga gacacgcaac cagaccggcg actgggatgc ggtcggccag tcgcgcaccg
      121 tcgtgctggt cggcggcggc agcatgcggg aacagttgac caggttcgat gcaccccatt
      181 cgttcgggta cacgctgtcc gaggtcaagg gtcccctgtc accgttggtc gaccacatcg
      241 acggcgagtg gatcttcgag ccgatcggcg cgggcaccaa cgtgacgtgg cggtggacca
      301 tctacccgcg cagtgggctc accgcgttcc tgctgccggt gttcggcaag ctgtggaagg
      361 gctacgcccg ccagtcgctg gaggagttgt ccaggcagct cgtgcactga ccggtcacgg
      421 gatgcgatag aacgggttcg gcagcaggta ggtgcgctcg gcgaacccgc catcgaggtc
      481 tgacggctgg tcgcccatgt tggcgacgat cgtgtagccc tcggactcga tcgtctgccg
      541 ttgcggcgcc ttgaaatcgg ctgccgaaag ataatgtgcg tcaacaggtt ccatgatcaa
      601 acgggaatag ccggtgtatc ccatggcggc cagattgcgt tcggtcgccg cgcgctgcga
      661 ctcggggcgc ccggtgatga agaagattgc cacgcccagg tccttcgcgg tgtcgtagac
      721 atccatcgtc ggcgggatca ccgtcgactg agcacgcaga tcccattcct gccagccgca
      781 cgggccttgc ggcaggtgca cacatgatcc accgatgacg cggccgaagt cgttggcttt
      841 gatggcttcc cagttcgaca acgcagtctc gtcgatgtcg aagacgacgg cggggcggtt
      901 cacccgcggg gcttcttcat tgagccaccc aatggccggc cacgcggcct gttgcaggtc
      961 ggtgaggtac gcgccgctgt cgtagtagtg cgtcgcctcg gttttcaggt cgccgatgtt
     1021 ggccggttga accggcggcg ggacgatcgg ggccggtggg gcaggcggtt cggcggccgc
     1081 catcggggcg aagccgatgg atgcggcggc cagtgcggcc gcgacgcgcg cgatcatcag
     1141 aaccggatca gcccgcgcag gttgaggccg ctgcgcaagt cgtcgtagcc ggagttgatg
     1201 tcggacagcg tgtattcgcg tgtgatgagc tcgtccagca tgagctgacc ggagtcgtac
     1261 agatcgagca tccggaagat gtcgatgcgc gggttggatg agccgaaaag cgaaccgcgg
     1321 agctgcttct cgaagagcgt cagatcgacc agagacaggt tcacctgctt ctcgtcgggg
     1381 tgtgccatcg cggtcatcac cacgcgtcct cgcttgccga ccaggctcag cgcctgcgcg
     1441 acgtaggcgc cctcagcgac gtcggtggtg acgacgcaca cgtccgcctt ctgcccgtgg
     1501 gtcagccccg tcaccagctt cgcggcctcc tcgagcgtcg ccgcggtgtg cgtggcaccg
     1561 aattccgttg cccgcgtgcg cttgtactcg acaggatcca gcgcgacgat gtgccgcgcc
     1621 ccgacgatcc gggccgcctg aaccgcgttg acgccgacac cgccgacccc catcaccacc
     1681 acggtgctgc ctgcggtggc ctccccggtg ttgaccatgc tgccgtatcc ggtcgtcacg
     1741 ccgcaaccga cgagcgccgc cttgtccagc gggacactgc ggtcgacctt gacgaccgac
     1801 gccatcggca cgacggtgta ctccgaaaac gttcccagta aacacatttg gcccacattg
     1861 tggccgcggg catggaaccg gtgggtgccg tcgagctggg gtcccctgag cagatgcatt
     1921 ccgagctcgc acaggttgct catgccgcgc gcacacggcc cgcaccgacc acacgcggga
     1981 atgaagctca gcaccaccga atccccctcg gcgacgtcgg tgacaccctc gccgacctcg
     2041 accacgacac cggcaccctc gtgtccgccg accaccggca tcgtcatgtg caggtcgccg
     2101 gtgaccaggt gctcgtcgga gtggcaca
//
LOCUS       DASYLX010000209         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_667707,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000209 DASYLX010000000
VERSION     DASYLX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667707"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VGP27_05030"
     CDS             <1..359
                     /locus_tag="VGP27_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7850715.1"
                     /translation="VLECLAAAQTLEEQGISAEVIDLRTLVPLDVDAMVESVRRTGRA
                     VVVHDAVQFAGPGAEIVAVLQAECFGELAAPIERVAARFTPTPAAAALESQIYPSAAR
                     IVKAVQRTLAKADAGG"
     gene            352..588
                     /locus_tag="VGP27_05035"
     CDS             352..588
                     /locus_tag="VGP27_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV7850716.1"
                     /translation="MADFIIRIPRVSVAVSEAELTELLVAAGERVAEGTPIYTIATEK
                     VEQEIEAGASGTVQWTGTVGTTYDIGAQIGVITS"
     gene            608..1471
                     /locus_tag="VGP27_05040"
     CDS             608..1471
                     /locus_tag="VGP27_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7850717.1"
                     /translation="MDMNETRERIIYQKDGPIAKITLNWPEKANAQDQKLAEEVDAAL
                     LDADRDYDIKVLVLKANGKGFCSGHAIGDNATDYPAFVENAMITGHPWKAQSDLFVKP
                     TLNLWEFSKPTIAQVHGYCVGGGTHMGLTTDIVIASEDAYFSYPPLQGFGMPSGECSI
                     EPWVFMNWRRAAYYLFTAEVIDAKKALEVGLVNEVVKREDLDARVDAIARHIAQAPLT
                     TLMLTKANLKRAWELMGMRVHWQSSNDLVALASISKDVQQLIQQVFKDKVLPSEHARR
                     QAAAASDGAAT"
     gene            1509..3044
                     /locus_tag="VGP27_05045"
     CDS             1509..3044
                     /locus_tag="VGP27_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7850718.1"
                     /translation="MNLADHANGDPDSPALIVANGRTITYGELFERSQRVAALLHRAG
                     LRRGDGVALVLPNRPEFFEITWGCQLSGLYYSAVNTHFTSDEVAYVIGDSDARAVFID
                     ASMADLGARILEVDDGVDVHISVGGALPGWPSYEDVLAESGPAPSISDGSEMLYSSGT
                     TGRPKAVRRPLPEDGQGSWAQKVLEYSLAQRYDMTPSSVYLSPAPLYHAAGINYTMAV
                     HRVGAASILMAKFDAETVLRLIDAHRVTHAQFVPTMFVRMLKLPEAIRERYDVSSLRC
                     VIHAAAPCPVDVKHRMMDWFGPIIHEYYGGTEGFAGTIIGPEEWLAHPGSVGKPLRPV
                     HVVSEGGAELPVGAPGELYFEGGPEFEYFKDPDKTASVSNDRGWRSLGDMGYVDEDGY
                     MYLTDRSTFMIVSGGVNIYPQEAENLLVMHPKLIDAAVFGVPNDEYGEEVKAVVQPVE
                     GFDAGPELEAELIDYCRANLATYKCPRTIEFDAELPRDPNGKLYKRRIRDRYWQDRTS
                     RIL"
     gene            complement(3117..4274)
                     /locus_tag="VGP27_05050"
     CDS             complement(3117..4274)
                     /locus_tag="VGP27_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV7850719.1"
                     /translation="MKSRAAILHDMGQQWSVEDFELDPPRAGEVLVEMAAAGLCHSDE
                     HIRNGDMSAPNEVMEAFGLPSMFPMIGGHEGSGVVLEVGEGVTEFEPGDHVVTSFVAV
                     CGQCRWCNSGMEYICDMGAQVMIPGMPTDGTFRHHTVDGRDLSHLSKVGAFSKHTVVS
                     PDSIVKIDRHLPLLPMALLSCGIPTGYGSAENRAKVRTGDTVVVIGVGGIGTGAVQGA
                     RINGAAQIIAVDPVEFKQKSALGFGATHSVSSIEEAADLVRDLTHGVMADSVVVSPSL
                     ITEPDVQAALSLTRKGGTCVLTGMTATTTSSIAINLQEFILWNKSLLGTVLGSCNPRV
                     DVARFAKLYEAGLLQLDEMITRRYRLDDINEGYRDQLNGEIVRGVIDFSLD"
     gene            complement(4271..>4502)
                     /locus_tag="VGP27_05055"
     CDS             complement(4271..>4502)
                     /locus_tag="VGP27_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850720.1"
                     /translation="PDVFDIQRPRRAHISFAGGIHSCLGMHLARVETKAMLTSLFDRV
                     TDLQLLEDDDTKIVGMPFRSPKHLPVTFRPVA"
BASE COUNT          844 a         1435 c         1470 g          753 t
ORIGIN      
        1 gtgtgctcga atgcctggcc gcggcgcaga cgctggagga gcagggcatc agcgccgagg
       61 tcatcgacct gcgaacgctg gtgccgctcg acgtcgacgc gatggtcgag tcagtgcgac
      121 gtaccgggcg tgcggtcgtc gtgcacgatg cggttcagtt cgcggggccg ggagcggaga
      181 tcgtcgccgt ccttcaggcg gaatgcttcg gtgagcttgc ggcgccgatc gaacgggtgg
      241 cggcaaggtt cacaccgacg cccgcggctg cggctctcga atcgcagatc tacccgtcgg
      301 cggcgcgcat cgtcaaggcc gtgcagcgaa cccttgcgaa agcggatgcc ggtggctgac
      361 ttcatcattc gcatcccgcg ggtgtcggtg gccgtgtcgg aggccgagct gaccgagctg
      421 ctggtcgctg cgggcgagcg cgtggcggag ggaacgccca tctacacgat cgctaccgaa
      481 aaggtggaac aggaaatcga agcgggtgca tcgggcaccg tgcagtggac gggcacggtt
      541 ggcaccactt acgacatcgg cgcccaaatc ggcgtcatca cgtcataacg aaacgccagg
      601 aggacacatg gatatgaacg agactcgcga gagaatcatc taccaaaaag acgggccgat
      661 cgcgaaaatc acgttgaact ggcccgagaa ggccaacgcg caggatcaga agctggccga
      721 ggaggtcgac gccgccctgc tggacgccga tcgggactac gacatcaagg tgctggtgct
      781 gaaggccaac ggcaagggct tctgctcggg acatgccatc ggcgacaacg ccaccgacta
      841 tccggcgttc gtggaaaacg ccatgatcac gggtcacccc tggaaggcgc agtccgacct
      901 cttcgtcaag ccgacgctga acctgtggga gttctccaag ccgacgatcg cgcaggtgca
      961 cggctactgc gtgggcggcg gcacgcacat ggggctgacc accgacatcg tgatcgcttc
     1021 cgaggatgcg tacttctcct acccaccgct gcagggcttc ggcatgccgt cgggcgaatg
     1081 ttccatcgag ccatgggttt tcatgaactg gcgccgagcc gcctactacc tgttcaccgc
     1141 cgaggtgatc gacgccaaga aggcgctgga agtgggcctc gtcaacgagg tggtcaaacg
     1201 cgaggacctc gacgcgcgcg tcgacgcgat cgcccgtcac attgcccagg ccccgttgac
     1261 gacgctgatg ctcaccaagg ccaacctcaa gcgggcctgg gagctgatgg gcatgcgggt
     1321 gcactggcag agctccaacg acctggtcgc gctcgcgtcg ataagcaagg acgtccagca
     1381 gctgatccag caggtgttca aggacaaggt gctgccgtcc gaacatgcgc gccgccaggc
     1441 tgcggccgcg tctgacggtg ccgccacgta gggcccggtg aacgagccgc gcacgtgagg
     1501 agcagaagat aaatctcgcc gaccacgcga acggtgatcc cgactcgccg gccctgatcg
     1561 tcgcgaatgg tcgcactatc acctacggcg agttgttcga acggagccaa cgcgtagcgg
     1621 ccctgctgca cagggcgggg ctgcggcgcg gcgacggcgt ggccctggtg ctgccgaatc
     1681 ggcccgagtt cttcgaaatc acctggggct gccagctttc cggcctctac tacagcgcgg
     1741 tgaacaccca cttcacgtcc gacgaggtgg cctacgtcat cggcgactcc gacgccagag
     1801 ccgtgttcat cgatgcctcg atggcggacc tcggggcacg gatcctcgag gtcgatgacg
     1861 gcgtcgatgt ccacatcagc gtcggcgggg cgctacccgg atggccgtca tatgaagacg
     1921 tcctggccga aagcggtcct gcgccatcga tttcggacgg gtccgagatg ctctactcgt
     1981 cgggcaccac cgggcggccc aaagccgttc ggcgaccgct gcccgaagac ggccaggggt
     2041 cgtgggcaca gaaggtgctg gagtactcgc tcgctcagcg atacgacatg accccgtcga
     2101 gcgtctacct ctcgcccgcg ccgctctatc acgccgcggg gatcaactac acgatggctg
     2161 tgcaccgagt gggcgccgcc tcgatcctga tggccaagtt cgacgccgaa accgtgctgc
     2221 gcttgatcga cgcacaccgc gtcacgcatg cccagttcgt gccgaccatg ttcgtgcgga
     2281 tgctcaagct gccggaggcg atccgggagc gctacgacgt ctccagcctg cggtgtgtca
     2341 tccatgcggc agcgccgtgc ccggtcgacg tcaaacatcg gatgatggac tggttcggtc
     2401 cgatcatcca cgagtactac gggggaacag agggattcgc gggcacgatc atcggccccg
     2461 aggagtggct cgcccacccc ggctcggtcg gcaagccgct gcgtcccgtg cacgtcgtca
     2521 gcgagggcgg tgccgaactg cctgtcggtg cgccgggcga gctgtacttc gagggcgggc
     2581 cggaattcga gtacttcaag gatcccgata agacggcatc ggtgtccaac gaccgcgggt
     2641 ggcgttcgct cggcgacatg ggctatgtcg acgaggacgg ctacatgtac ctcaccgacc
     2701 gctcgacgtt catgatcgtc tccggcggag tgaacatcta cccgcaggag gccgagaacc
     2761 tgctcgtcat gcacccgaag ctgatcgacg ccgcggtgtt cggtgtgccc aatgacgagt
     2821 acggcgaaga ggtcaaggcc gtcgtgcaac cggtcgaggg cttcgacgcc gggccggagt
     2881 tggaggccga gctcatcgac tactgcaggg cgaatctcgc cacgtataag tgcccgcgca
     2941 ccatcgagtt cgacgccgag ctgccgcgcg atccgaacgg caagctgtac aagcggcgga
     3001 tccgcgaccg ctactggcag gaccgcacat cccgaatcct ctgacccacc gacgccgaaa
     3061 ctcacgtttg ggcgcgaaag tgcgagtaaa tcgcgccaat tcgtcggttt cggcgactag
     3121 tcgagactga aatcgatgac gccgcggacg atttcgccgt tgagctggtc gcgatagccc
     3181 tcgttgatgt cgtcgagccg gtaacgccgg gtgatcatct cgtcgagttg caggagtccc
     3241 gcctcataga gcttcgcgaa acgagcgacg tccacccgcg gattgcacga ccccagcacc
     3301 gtgcccagca ggctcttgtt ccacaggatg aactcctgca ggttgattgc gatggaactc
     3361 gtcgtggtgg cggtcatgcc ggtcagcaca caggttccgc ccttgcgcgt caggctcagc
     3421 gccgcctgga cgtctggctc cgtgatcaac gagggggaga cgacgacact gtccgccatc
     3481 acgccgtgcg tcaaatcgcg gaccaggtcg gcggcctcct cgatgctgga aacgctgtgc
     3541 gttgcaccga atcccagagc cgacttctgt ttgaactcca cagggtcgac cgcgatgatc
     3601 tgcgcggccc cgttgatgcg tgctccctga acggcgccgg taccgatgcc tccgacaccg
     3661 atgacgacga cggtgtcgcc ggttcttacc ttcgcgcggt tctccgcgga gccgtacccg
     3721 gtcggaattc cacacgacaa cagcgccatt ggcagcaacg ggaggtggcg atcgatcttg
     3781 acgatcgaat ccggcgaaac cacagtgtgt ttcgagaacg caccgacctt ggacaagtgg
     3841 ctgaggtcac ggccgtccac ggtgtgatgg cggaaggtgc cgtccgtcgg cataccggga
     3901 atcatcacct gtgcacccat gtcacagatg tattccatgc cggagttgca ccatcgacac
     3961 tggccgcaca ccgcgacgaa cgatgtcacg acgtgatcgc ccggctcgaa ctcggtcacg
     4021 ccctcgccca cctccagcac cacacccgag ccttcgtgac cgccgatcat cgggaacatg
     4081 gagggcaggc cgaaggcctc catcacctcg ttcggcgcag acatgtcgcc gttacggatg
     4141 tgctcatccg aatggcagag accggcagcg gccatctcga cgagcacctc gcccgcccga
     4201 ggggggtcga gctcgaagtc ttcgactgac cactgctgac ccatgtcgtg caggatcgcc
     4261 gcccggctct tcatgcgacc ggccggaagg tcacggggag gtgcttgggg gagcggaacg
     4321 gcatgccgac gatcttggtg tcgtcgtctt ccagcagctg tagatccgtc acacgatcga
     4381 agaggctggt gagcatggct ttggtctcga cccgcgcgag gtgcattccg aggcagctgt
     4441 gaatcccgcc cgcaaacgag atgtgcgcgc gacggggtcg ctgaatgtcg aagacgtcgg
     4501 gg
//
LOCUS       DASYLX010000210         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_9172, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000210 DASYLX010000000
VERSION     DASYLX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9172"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2792)
                     /gene="ppc"
                     /locus_tag="VGP27_05060"
     CDS             complement(<1..2792)
                     /gene="ppc"
                     /locus_tag="VGP27_05060"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV7850721.1"
                     /translation="MADGPDTSLAPIGSVQRTKLGREATEPMREDIRLLGTILGDTVR
                     EQNGDEVFDLVERARVESFRVRRSEIDRAELAGMFDGIDIHRAIPVIRAFTHFALLAN
                     VAEDIHRERRRAVHVASGQPPQDSSLAATYDKLDSAELDSATVAETLRGALVSPVITA
                     HPTETRRRTVFDTQHRITELMRLRLHSHIHTADGRDIETELRRNILTLWQTALIRLSR
                     LKIGDEIETGLRYYPAAFLEVIPKVNAEVRAALQARWPDAHLLDEPILRPGSWIGGDR
                     DGNPNVTSDVVRLATSRAAYTALAHYFVEITALEEELSMSARLVTVSDELVALADACE
                     EPARADEPYRRALRVIHGRLTATAQQVLDEQPEHELHLGLERYESPGELLTDLDVVDG
                     SLRANGSAVLADDRLARLREAVRTFGFHLSGLDMRQNSDVHEEVVAELLAWAGVHPDY
                     RSLPEPDRVDLLVAELGTRRPLIGDGAELSELARKELDICGAAARAVNVFGSLAVPNY
                     VISMCQSVSDMLEAAVLLKEVGLIDASGPDAYCPVSIVPLFETIDDLQRGSSILEAAL
                     DLPLYRSLVASRGDSQEVMLGYSDSNKDGGYLAANWALYRAELDLVESARKTGIRLRL
                     FHGRGGTVGRGGGPSYDAIRAQPPGAVNGSLRLTEQGEVIAAKYAEPRVAHRNLETLL
                     AATLEASLLDVEGLGDQAEPAYEVLDDLAARAHRAYAELVHETPGFVDYFKASTPVGE
                     IGALNIGSRPTSRKPTTSISDLRAIPWVLAWSQSRVMLPGWYGTGTAFEEWISEEDGR
                     LELLQDLYRRWPFFRTVLSNMAQVLAKSDMGLAARYAELVDDEALRRRVFDKIVDEHD
                     RTIRMHEAITGHDDLLADNPALARSVFNRFPYLEPLNHLQVELLRRYRSGDDDELVQR
                     GILLTMSGLATALRNSG"
BASE COUNT          463 a          954 c          995 g          481 t
ORIGIN      
        1 ccgctgtttc tgagcgcggt cgccagcccg ctcatcgtca gcagaatgcc gcgctgaacg
       61 agttcgtcgt cgtcgccgga gcggtaccgc cgcaacagtt ccacctgaag gtggttcaac
      121 ggctccaggt acgggaaacg gttgaacacc gaccgtgcca acgcggggtt gtcggcaagc
      181 aggtcatcgt gcccggtgat cgcctcgtgc attcggatgg tgcggtcgtg ctcgtcgacg
      241 atcttgtcga acacccgccg acgcagcgcc tcgtcatcga ccaactccgc gtaacgcgcc
      301 gccaggccca tatccgactt cgcgagcacc tgagccatgt tcgacagcac cgtgcggaag
      361 aacggccacc gccggtagag atcctgcagc agttccagcc tgccgtcttc ctctgagatc
      421 cactcctcga acgcggtgcc ggtgccatac cagcccggca gcatgacacg cgactggctc
      481 cacgccagta cccacgggat cgctcgtagg tcggagatcg acgtggtcgg tttgcgcgac
      541 gtcggcctgc tgccgatgtt cagcgcgccg atctcgccga ccggcgtgga cgccttgaag
      601 tagtcgacga aacccggtgt ctcatggacc aattcggcgt aggcgcggtg cgcgcgagcg
      661 gccagatcgt cgagcacctc gtaggcgggt tcggcctgat cgccgagccc ttcgacatcg
      721 agcagcgacg cctcaagggt tgcggcgagc agggtctcca ggttgcggtg cgcgacccgc
      781 ggctccgcgt acttggcggc gatgacctcg ccctgctcgg tgagccgcaa cgacccgttc
      841 accgcgccgg gcggctgcgc ccgtatcgcg tcgtagctgg ggccgccgcc gcgcccgacg
      901 gtgccgccac gaccgtggaa cagccgcaac cggattcccg tcttgcgggc ggactcgacg
      961 aggtcgagct cggcgcggta caacgcccag ttggccgcca gatagccgcc gtccttgttg
     1021 gagtcggagt agccgagcat gacctcctgg ctgtccccgc gcgaggccac caacgaccgg
     1081 tacagcggaa ggtcaagcgc cgcttcgaga atggacgagc cgcgctgcag atcgtcgatg
     1141 gtctcgaaca gcggcacgat actcaccggg cagtatgcgt ccggtccgga cgcgtcgata
     1201 aggcctacct ctttcagcag cacggccgcc tcgagcatgt ccgatacgga ctggcacatc
     1261 gagatgacgt agttggggac ggcgagcgag ccgaacacgt tgacggctcg ggcggcggcc
     1321 ccgcagatgt cgagttcctt gcgtgcgagc tcggacagtt cggcgccgtc accgatcagc
     1381 ggccgccggg tgccgagctc ggccaccagc aggtcgaccc ggtcgggttc gggcagcgaa
     1441 cggtagtcgg gatgtacgcc cgcccatgcc agcagctccg cgacgacttc ctcgtgcaca
     1501 tcggagtttt gccgcatatc aagcccggac aggtgaaagc cgaaggtgcg cacggcttcc
     1561 cgtagccgcg cgagccggtc gtcggctagt accgcgctgc cgttggcccg cagcgagccg
     1621 tcgacgacgt ccagatcggt cagcagctcc cccggcgatt cgtagcgctc gaggccgagg
     1681 tgtaactcgt gctcgggctg ctcgtcgagc acctgctggg cggtggcggt gagcctgccg
     1741 tggatgaccc gcagcgcacg ccggtagggc tcgtcggccc gtgccggctc ctcgcacgcg
     1801 tcggccagcg ccaccagctc gtcgctgacc gtgaccagcc gcgccgacat cgacagctct
     1861 tcctccagcg cggtgatctc cacgaagtag tgcgcgagcg cggtgtaggc ggcgcgagag
     1921 gtggccaggc ggacgacgtc ggaggtgacg ttcgggttgc cgtcgcggtc gccgccgatc
     1981 cacgacccgg gccgcaggat cggctcgtcg agaaggtggg catccggcca gcgagcctga
     2041 agcgctgcgc gcacttcggc gttcaccttc gggatgactt ccagaaacgc agccgggtag
     2101 tagcgcagac cggtttcgat ctcgtccccg atcttcaacc gcgacagtcg gatcaacgcg
     2161 gtctgccaga gcgtcaggat gttgcgccgc agttcggtct cgatgtcgcg accatcggcg
     2221 gtgtgaatgt gggagtgcag gcgcaaccgc atcagttcgg tgatgcggtg ctgggtgtcg
     2281 aagaccgtgc ggcgtctggt ctcggtgggg tgcgcggtga tcaccggtga caccagtgca
     2341 cccctcagcg tctcggctac ggtcgcggag tccagctccg cggaatcgag cttgtcgtag
     2401 gtggcggcca ggctgctgtc ctgtggcggc tggcctgacg ccacgtggac ggcgcggcgg
     2461 cgctccctgt ggatgtcctc ggcgacgttc gccagcaatg cgaaatgggt gaacgcgcgg
     2521 atgacgggga tggcccggtg gatgtcgatg ccgtcgaaca ttccggcgag ctctgcccgg
     2581 tcgatctcgg agcggcgcac ccggaaggac tcaacccgtg cccgctcgac gaggtcgaag
     2641 acctcatcgc cgttctgctc gcggaccgtg tcgcccaaga tggtgccgag gagccgaatg
     2701 tcttcccgca tcggctcggt ggcttcgcgg ccgagcttgg tgcgttgcac agagccgatc
     2761 ggggccagtg aggtgtcggg tccgtcagcc attacccgat tatcggcgac cgggaatcag
     2821 cctgcagggc gggacgcctg tgtcagatgg cagccaccac gatcgtgagg aggatcacca
     2881 ggaacaggag tac
//
LOCUS       DASYLX010000211         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1874027,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000211 DASYLX010000000
VERSION     DASYLX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874027"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..605
                     /locus_tag="VGP27_05065"
     CDS             180..605
                     /locus_tag="VGP27_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV7850722.1"
                     /translation="MLAAVPAQADGGDQGGVVLSEINATRAANGCGPVAANPQLTQSA
                     ARQANDMLANGVTDHTGSDGSSLVQRVTDAGYAPYASLGEVIFWSSGLSGAPAAAVSW
                     WMNSPGHRAIITDCTLTDAGFSAVSNGGNMTAAGDFGRK"
     gene            802..1725
                     /locus_tag="VGP27_05070"
     CDS             802..1725
                     /locus_tag="VGP27_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV7850723.1"
                     /translation="MDMKKCSTWVPICAAAVTVLGGALIAPPVHADGGCIPGNQAGMV
                     PRNARPGDNACVSPDIATLVQQENGTKADGYAAGAASTDLICKSGLVWREAFDGDAVC
                     VSPERRQATFQENANAGVGNTGGLQPEAGVTAGGGPAAQGTGGPDQALLALVNDARLH
                     PEKYPLIGDYAGNPTDTSAAHSTACAKPFGRSGALENSAATHNNYIATIPKPGLDGGT
                     RAHETPGGGQVGDPGGPLARAGYGHNGEIVAAEWADDAAAVKYWMSLDGPGWTHRNAV
                     LDCTLTDAGAASFSAPGSAYQRYYTVDMGSH"
     gene            1995..3011
                     /locus_tag="VGP27_05075"
     CDS             1995..3011
                     /locus_tag="VGP27_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEV7850724.1"
                     /translation="MARTKMVRRWRRNMDVVDDTEYTDMLTTLSEGSVRRNFNPYTDI
                     EWGSPEFAVIDNDERWILPTTDPVGRHSWYKAQPLERQIEIGMWRQANVAKVGLHFES
                     ILIRGLMEYAFWTPNGSPEYRYCLHEAVEECNHTMMFQEMVNRIGADVPGMPRLLKWI
                     QPFIPLVAGPLPIPFWFGILAGEEPIDHTQKNVLREGKTLHPIMERVMAIHVAEEARH
                     ISFAHEYLRKRVPHLPRRKRFWLSLFVPVTMRILCSAIIVPPRAFWKEFDIPRSVRKE
                     IFFRSPESRQMLRDMFGDVRMLCHDTGLINPIAKLVWRICRIDGRPSRYRSEPARQHL
                     VSAA"
     gene            3036..>3598
                     /locus_tag="VGP27_05080"
     CDS             3036..>3598
                     /locus_tag="VGP27_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV7850725.1"
                     /translation="MPHVITQSCCSDGSCVYACPVNCIHPSPDEPGFATAEMLYIDPV
                     ACVDCGACVSACPVGAIAPDTKLEPKQLPFVALNAAYYPDRPAGEKLPPTSKLAPVID
                     APKVHVRGANPLTVAIVGSGPAAMYAADELLTQQGVHVNVFERLPTPYGLVRAGVAPD
                     HQSTKRVTRLFDKIAKRRGFRFFLNVEV"
BASE COUNT          671 a         1128 c         1179 g          620 t
ORIGIN      
        1 ggccggtgtg tgagaacgcg ggaaatatgc caaacacccg attcgtcggc accggctgtg
       61 tccatactgt gactggtcgg gactgcaatt cgagcccgat gtggggtcgc gttatgaaca
      121 acggaaaagc ctcgacgctg aaaacgctct gtgcagcggc ggtaatggtt ctttgtccgg
      181 tgctcgccgc ggtgccggcc caggccgacg gcggtgacca gggcggcgtc gtgctgagcg
      241 aaatcaacgc cacgcgcgcg gccaatggat gcggccctgt tgcagccaac ccgcagttga
      301 ctcagtccgc cgcgcgtcag gccaacgaca tgctcgccaa cggggtgacg gaccacaccg
      361 gctccgacgg ttcctccctg gtgcagcgcg tcacggacgc cggctacgcg ccgtacgcct
      421 cgctcggcga ggtcattttc tggagcagcg gcttgagcgg cgcgcccgcc gcggcggtgt
      481 cctggtggat gaacagtcct ggccaccgcg cgatcatcac cgactgcaca ctcaccgatg
      541 ccgggttctc cgcagtgagc aacggcggca acatgacggc agccggagac ttcggaagga
      601 agtagcgggt cggcaagcac tcatagttca gctcgttccg cgcccgccgt cgaggtttga
      661 acgtggtcgc aaaacattcg aagcggcgca gaagccaagg cagaaacata gggaaacacc
      721 cgatttctcg aacatcgctg cgtacctact gtgacttgtg ttcggagttc atttctgact
      781 cgagttcgga aggacggcga catggatatg aagaagtgct caacctgggt gccgatctgt
      841 gctgcagcgg tcaccgtttt gggtggagcc ctgatcgcgc cgccggtcca cgcggacggc
      901 gggtgcattc ccggcaatca ggcggggatg gtgccgcgca acgcccggcc cggcgacaac
      961 gcgtgcgttt cgccggacat cgcgacgctg gtccagcagg agaacgggac caaggccgac
     1021 ggatacgcgg cgggcgccgc gtcgacagac ttgatctgca agtcgggcct ggtatggcgc
     1081 gaggcgttcg acggcgacgc tgtctgcgtc agtcccgagc ggcggcaagc aacattccag
     1141 gagaacgcca atgccggcgt cggtaacaca ggcgggttgc aacccgaggc cggtgtgacc
     1201 gccggcggcg gcccggccgc gcaaggcacg ggcggacccg atcaggcgct gcttgcactt
     1261 gtcaacgatg cgcggctgca cccggagaag tacccgctga tcggggatta cgccggaaac
     1321 ccgacggata cgagcgccgc gcattcgacc gcgtgtgcca aaccgttcgg tcgctcgggg
     1381 gcgttggaaa attcggccgc cacgcataac aactacatcg cgacgatccc gaagcccggc
     1441 ctcgacggcg gcacgcgcgc acacgaaacg ccaggcggcg gccaagtggg cgatcccggt
     1501 ggaccgctcg cccgagccgg gtatggccat aacggagaga tcgtcgcggc cgaatgggcc
     1561 gacgatgcgg ctgctgtgaa gtactggatg tcgttggacg gcccagggtg gactcatcga
     1621 aacgcggtac tggactgcac tttgactgac gccggggcgg ccagcttctc agcgccaggc
     1681 agtgcttacc agaggtatta caccgtcgac atgggcagcc actagaccta actgctcggc
     1741 actcgggcac cgtcgcgggt cacgcgacgg tgtgctcgct gtgtgccgcg ggcgctcgac
     1801 gccgtgcgga tcgtcctgtt ggaatcgtgg acgtaatcgt cggggcgtcg tcacgttatt
     1861 gtgatctgcg tcacagatcc ggtcgggcag gcccgtttga cacatcgcgc atgggggcat
     1921 ttcaaatatg cgatacctgc ggtaccgttg atggacacga aggccgcaaa tgtaaacctc
     1981 aggggaggct tctgatggct cgtacgaaga tggtcaggcg ctggcgtcgc aacatggatg
     2041 tcgtcgacga caccgaatac accgacatgc tgaccacgct gtccgagggg tcggtgcggc
     2101 gtaatttcaa tccgtacacc gacatcgagt ggggctcccc cgagttcgcg gtgatcgaca
     2161 acgacgagcg atggatcctg cccacgacgg atccggtcgg ccggcacagc tggtacaagg
     2221 cgcaaccgct cgagcgtcag atcgagatcg gcatgtggcg acaggccaac gtcgcgaagg
     2281 tcggcctgca cttcgagtcg atcctgatcc gtggcctgat ggagtacgcg ttctggacgc
     2341 ccaacggctc gccggaatac cggtactgcc tgcacgaggc ggtcgaggaa tgcaaccaca
     2401 cgatgatgtt ccaggagatg gtgaaccgca tcggtgccga tgtgccgggg atgccgcggc
     2461 tgctcaagtg gatccagccg ttcatcccgc tggtcgcggg gcccctaccg attccgttct
     2521 ggttcggcat tctcgccggc gaagaaccga tcgaccatac acagaaaaac gttctgcgcg
     2581 aagggaaaac gctgcatccg atcatggaac gggtgatggc catccacgtc gccgaggaag
     2641 cccggcacat ctcgttcgcg cacgagtatc tgcgcaagcg agttcctcac ctgccgcggc
     2701 ggaagcggtt ctggctgtcg ctcttcgttc cggtgacgat gcggatcctg tgctcggcca
     2761 tcatcgttcc gccgcgggcg ttctggaagg aattcgacat cccacgctcg gtgcgcaagg
     2821 aaatcttctt ccgatcgccc gaatcccgac agatgctgcg cgacatgttc ggtgacgtcc
     2881 ggatgctctg ccacgacacc ggtctgatca acccgatcgc caagctggtg tggcggatct
     2941 gcagaatcga cggcaggccc agccgttacc gcagcgaacc cgcccgccaa catctggtgt
     3001 cggccgccta agcgcaaccc acgccggaac tttctatgcc ccatgtgatc acccagtcgt
     3061 gctgtagcga cgggtcctgt gtctatgcct gcccggtgaa ctgtattcat ccgtcaccgg
     3121 acgaaccggg cttcgcgacc gccgagatgc tctacatcga tccggtcgcc tgcgtcgact
     3181 gcggtgcgtg cgtgagcgcg tgcccggtcg gcgcgatcgc gcccgacacg aagctcgagc
     3241 cgaagcagct gcccttcgtc gcgctcaacg cggcgtacta cccggaccgg cccgcaggtg
     3301 agaaactgcc tccgacgtcg aaattggcgc cggtgatcga cgcgccgaaa gtgcatgtgc
     3361 gcggggcgaa tccgttgact gtggcgatcg tcggctccgg acccgctgcg atgtacgcag
     3421 ccgacgaact gctgacccaa cagggtgtgc atgtcaacgt cttcgaacgg ctgccaactc
     3481 cgtatggcct ggtgcgtgcg ggcgtggcgc ccgaccacca gagcaccaag agggtcaccc
     3541 ggctgttcga caagatcgcc aagcgacggg gcttccggtt tttcctcaat gtcgaggt
//
LOCUS       DASYLX010000212         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3629280,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000212 DASYLX010000000
VERSION     DASYLX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629280"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VGP27_05085"
     CDS             complement(<1..107)
                     /locus_tag="VGP27_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559646.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV7850726.1"
                     /translation="MAVDTAAGSNGAGGQTGTARVKRGMAEMLKGGVIM"
     gene            complement(195..1229)
                     /locus_tag="VGP27_05090"
     CDS             complement(195..1229)
                     /locus_tag="VGP27_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7850727.1"
                     /translation="MTWLVVTTLVVLLVVLLLVGAWAYQTAHRLDRLHVRYDLSWQAL
                     DGALARRAVVARAVAVDAYGAGPEGRRLKALADAAERAPRAARESTENELSAALALVD
                     PASLPVALVAELADAEARVTLGRRFHNDAVRDTLALRERLAVRLLRLGGTAALPGYFE
                     IAEHADRDVGMVSRRTSARVVLLDERRAVLLFCGSDPAQPDGNDEPAPRWWFTVGGAA
                     QPGETLAEAAVRELAEETGLRVEPADMTGPVWRRDAVIDFNGSVIRSEEMFFVHRTSR
                     FEPSASGRTALELRYIHGHRWCDETIIEELVAGGEAVYPLQLGELLAEANALADARGA
                     GPQRQLQSIR"
     gene            complement(1229..>2089)
                     /locus_tag="VGP27_05095"
     CDS             complement(1229..>2089)
                     /locus_tag="VGP27_05095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7850728.1"
                     /translation="NAPSLSMLALQAAEGPIVATFHTSTTKSLTLSVFQGILRPYHEK
                     IIGRIAVSDLARRWQMEALGSDAVQIPNGVDVASFASAPLLDGYPRPGKTVLFLGRFD
                     EPRKGIAVLMAALPALVERFPDIEVLIVGRGDEDELREKTGPLAEHLRFLGQVDDAQK
                     ASAMRSADVYCAPNTGGESFGIVLVEAMAASTAVVASDLDAFRRVLEDGKAGRLVPVD
                     DADGLAAGLIEVLEDDAARGGFIEAAGKTVHQYDWSVVAGQITRVYETVAGAGAKVQV
                     AGSAGRSVAT"
BASE COUNT          335 a          790 c          658 g          306 t
ORIGIN      
        1 tccatgatga cgccaccctt gagcatctcg gccatgccac gcttgacacg ggcagtgccg
       61 gtctgaccgc ctgccccgtt tgatccggct gcggtatcca ctgctatctc ctccaaatgg
      121 ctactgatcc agtctaaagg gagcaccaaa tccaataaat ccggtgcgca cgcgaggagc
      181 agaaacgatt ccggtcagcg gatcgactgc aactgccgct gcggcccggc gcctcgggcg
      241 tcggccagcg cgttcgcctc cgcaagcaac tcgccgagtt gcagcgggta gacggcctcc
      301 cccccggcga ccagctcctc gatgattgtc tcatcgcacc agcggtgccc gtggatgtag
      361 cgcagttcca gtgcggtccg ccccgacgcg gacggctcga accggctggt ccggtgcacg
      421 aagaacatct cctcgctgcg gatgacggag ccgttgaagt cgatgacggc gtcccgtcgc
      481 cacaccggcc cggtcatgtc ggccggctcc acgcgcaggc cggtctcttc ggccagctcc
      541 cgcaccgcag cctcggccag cgtctctccc ggctgggccg cgccgcccac ggtgaaccac
      601 caccgcggtg ccggctcgtc gttgccgtcc ggctgcgccg ggtccgagcc gcagaacagc
      661 agcaccgctc tgcgctcgtc cagcagcacc acgcgggccg acgtccggcg gctgaccatg
      721 ccgacgtcgc ggtcggcatg ctctgcgatc tcgaaatagc ctggcagcgc ggccgttccg
      781 ccgagccgaa gcaagcgcac cgccagccgc tcgcgcagtg ccagtgtgtc gcgcaccgcg
      841 tcgttgtgaa accggcggcc cagtgtcaca cgcgcctcgg cgtcggccag ctcggcgacg
      901 agcgccaccg gcagcgacgc cgggtccacc aacgcgagcg ccgccgacag ctcgttctcg
      961 gtggattccc tcgcggctcg cggggcgcgc tcggcggcgt cggccagcgc cttcagccgc
     1021 ctgccttcag ggcctgcgcc gtaggcgtcg acggcgaccg cgcgggcgac cacggcgcgg
     1081 cgggcaaggg caccgtcgag cgcctgccag gacaggtcgt agcggacatg tagccggtcc
     1141 agccggtgcg ccgtttgata cgcccacgct cccaccagca gcaacaccac aagcagcacg
     1201 acaagcgtcg tcaccacgag ccaggtcatc aggtcgctac gctcctaccc gccgaacccg
     1261 ccacctgcac ctttgccccc gcgccggcaa ccgtctcgta gacccgcgtg atctggcccg
     1321 ccaccaccga ccagtcgtac tgatgcactg tcttgccggc cgcctcgatg aagccgccgc
     1381 gggcggcatc gtcctcgagc acctcgatga ggccggcggc gagcccgtcg gcgtcgtcga
     1441 ccggcaccag ccgcccggcc ttgccgtcct cgagcacgcg gcggaacgcg tcgaggtcgc
     1501 tggccaccac ggcggtactc gcggccatcg cttcgacgag cacgataccg aagctctcgc
     1561 cgccggtgtt gggtgcacag tagacgtcgg cgctgcgcat cgcggacgcc ttctgcgcat
     1621 cgtcgacctg gccgaggaaa cgaaggtgct cggccagcgg cccggtcttc tcacgcagct
     1681 cgtcctcgtc gccgcggccc acgatcagga cctcgatatc gggaaagcgc tccaccaatg
     1741 ccggtagcgc cgccatcagt accgcaatgc ccttgcgcgg ctcgtcgaac cggccgagga
     1801 acagcaccgt cttgcccgga cgcggatatc cgtccagaag cggcgccgac gcgaacgacg
     1861 cgacgtcgac tccgttgggt atctggacgg cgtcagagcc cagcgcctcc atctgccagc
     1921 gccgagccag atcggacacc gcgatacgac cgatgatctt ttcgtggtag ggccgcagga
     1981 tgccctgaaa aacactgagc gtcaacgact tcgtcgtcga cgtgtgaaac gtcgcaacga
     2041 tcggcccctc ggcggcctgc agcgccagca tcgacaggct cggcgcgtt
//
LOCUS       DASYLX010000213         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_777599,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000213 DASYLX010000000
VERSION     DASYLX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777599"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGP27_05100"
     CDS             <1..379
                     /locus_tag="VGP27_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7850729.1"
                     /translation="NLSRGTVDWESVGGAYAESMVAAVADRLPALGVDTELLHVVNPA
                     DWAEQGMVAGTPFALAHTFGQTGPFRPANTVRGIENVVLAGSSTVPGVGVPTALLSGR
                     LAADRVTGAVTRSVATHIGSGHR"
     gene            376..1320
                     /locus_tag="VGP27_05105"
     CDS             376..1320
                     /locus_tag="VGP27_05105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEV7850730.1"
                     /translation="MIRSELDAAGVRDPALRDAYQQCRLLNAEHGRTFFLATRLLAPK
                     QRPAVHALYGFARRADDILDDFDAGASAVERAERLQRLATQLFNQLVQQQDCGDDPAL
                     RAVVHTARRYGIPWEMFDDFLASMRMDLTVTDYPDRKALERYMHGSAEVIGLQMLPVL
                     GTVCPREEAAPYAAALGKAFQLTNFLRDVDEDLQRGRVYLPADELAAYRVDRNMLTWC
                     HTNRRTDVRVRKALTEQHAATRRVYEVARKGIEMLDPRSRPCVAAAYTLYSEILDRIE
                     NIDFEIFDQRATVGTGRRLQVAGIGLWRARRARLTHGR"
     gene            1330..1677
                     /locus_tag="VGP27_05110"
     CDS             1330..1677
                     /locus_tag="VGP27_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEV7850731.1"
                     /translation="MDRWQYLIVLGACLLITAPLELFGQGVYRQARRAAFAILPVAVV
                     FVVWDAVAIAAHIWTYNPRYVSGIELPAHIPVEELLFFIVIPLCGLFTYNAVDTILTY
                     LQKKRRARSEQST"
     gene            1674..2006
                     /locus_tag="VGP27_05115"
     CDS             1674..2006
                     /locus_tag="VGP27_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEV7850732.1"
                     /translation="MTGLGYTLPAVLAVIAVIAVEFGLLHTGLFRRPAYWISMVIVFG
                     FQIPVDGWLTKLSAPIVIYDDSHTSGIRVPFDIPVEDFLFGFALVTGVLLLWERQRLR
                     DRKTRSAK"
     gene            2078..2767
                     /locus_tag="VGP27_05120"
     CDS             2078..2767
                     /locus_tag="VGP27_05120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7850733.1"
                     /translation="MPAAFDAGAAAYDGLVDANPGYHRHLRLSAERMRLVNGGKGLRL
                     LDAGCGTGASTAALLSVAPQADIVAVDASSGMLAEAAAKHWPASVRFVHSRIEDIAEA
                     GVEGPFDGIFAAYLLRNLEDPDAQLREFLALLRPGATLAVHEYSVRDSRVAAVMWNAV
                     CATIIIPMGRLRSGDAGLYRYLRRSVNEFDGAETFLNRLRDNGFTSARSETVPGWQRN
                     IVHTFLAEAPA"
     gene            2764..>2919
                     /locus_tag="VGP27_05125"
     CDS             2764..>2919
                     /locus_tag="VGP27_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isorenieratene synthase"
                     /protein_id="HEV7850734.1"
                     /translation="MTDPRRASHPAAPGLPDAGSLGSTPHVVVVGGGIAGLAAATGLI
                     ERGVTVDV"
BASE COUNT          480 a          989 c          967 g          483 t
ORIGIN      
        1 gaacctttcg cggggaaccg tcgactggga aagcgtcggc ggcgcctacg ccgagagcat
       61 ggtggccgct gtcgccgatc ggctcccagc gctcggggtc gataccgaac tgctgcacgt
      121 cgtcaacccg gcggactggg ccgaacaggg catggtggcc ggcacaccgt tcgcgctggc
      181 gcacacgttc ggccagacag gaccgttccg cccggccaac acggttcgcg gcatcgagaa
      241 cgtcgtgctg gccggctcgt cgacggtgcc gggcgtcggc gttccgaccg ccctgctgtc
      301 gggacggctt gcggccgacc gggttaccgg tgcagtcacc cgcagcgtgg caactcacat
      361 cgggagtggc caccgatgat tcgctcagag ctcgacgccg cgggtgtgcg cgatccagca
      421 ttacgtgacg cctaccaaca gtgccggctg ctcaacgccg aacacggccg aacattcttc
      481 ctcgccacca ggctgttggc gccgaaacag cggcctgccg ttcacgcgct gtacggcttc
      541 gcgcggcgtg ccgacgacat cctcgacgac ttcgacgccg gcgccagcgc ggtcgaacgg
      601 gccgaacggc tccagcgtct cgccacccaa ttgttcaatc agcttgtcca acaacaggac
      661 tgcggtgacg atcccgccct gcgggccgtc gtccacaccg cgcgccggta cggcattccc
      721 tgggagatgt tcgacgactt cctggcatcc atgcggatgg acctgacggt caccgactat
      781 cccgaccgca aggcactcga gcgttacatg cacggatcgg ccgaggtgat cgggctccag
      841 atgctgccgg tgctgggcac tgtctgtccg cgcgaggagg ccgcgccgta tgccgccgcg
      901 ttgggaaagg cgttccagct caccaacttc ctgcgtgacg tcgacgagga tctgcagcgt
      961 ggccgggtgt atctacccgc cgacgagttg gccgcgtacc gcgtcgaccg gaacatgctg
     1021 acgtggtgtc acaccaacag gcgcaccgac gtccgggtgc gcaaggcact caccgaacag
     1081 catgccgcca cccgccgggt ctacgaagtg gcccgcaagg gcatcgaaat gctcgacccg
     1141 cggtcgcgac catgcgtcgc agcggcatac acgctgtact cagagatcct ggaccgcatc
     1201 gagaacatcg acttcgagat cttcgaccag cgcgcgaccg tcgggaccgg caggcggctg
     1261 caggtggcgg ggatcggttt gtggcgggcc cggcgcgcgc ggttgacgca cggtaggtaa
     1321 gaactagaca tggaccgctg gcagtatctg atcgtgctgg gcgcctgcct gctcatcacc
     1381 gcgccgctgg agctattcgg tcaaggcgtg taccggcagg cgagacgcgc cgccttcgcc
     1441 atactgcccg ttgcggtcgt attcgtggtg tgggacgccg tggcgatcgc cgcgcacatc
     1501 tggacataca acccgcgcta cgtcagcggc atcgagctgc ccgcccacat ccccgtcgaa
     1561 gaactcctgt tcttcatcgt gatcccgctc tgcgggctgt tcacctacaa cgccgtcgac
     1621 accatcctga cgtacctgca gaagaagaga cgcgctcgat cggagcagtc gacgtgacgg
     1681 gactcggtta cacgttgccg gccgtgctgg cggtgatcgc ggtgatcgcg gtggagttcg
     1741 gcctgctcca caccggcctt ttccgccgcc ctgcctactg gatctcgatg gtgatcgtgt
     1801 tcggcttcca gatacccgtc gacggctggc tcaccaaatt gtccgccccg atcgtcatct
     1861 acgacgacag ccacaccagc ggcatcaggg ttcccttcga cattcccgtc gaggatttcc
     1921 tgttcggctt cgccctggtg accggggtgc tgctgctgtg ggagcgtcag cggctacgcg
     1981 atcgcaagac caggagtgcc aaatgacgga cagaaaatct tcgcggacgc gaggagcaca
     2041 gaaaacgtcg cggacgcgag gagcacagaa aacagaggtg cccgccgcgt tcgacgcggg
     2101 cgcggcggcc tacgacggcc tggtcgatgc caaccccggc tatcaccgcc accttcggtt
     2161 atccgccgag cggatgcgtc ttgtcaacgg cggcaaggga ctacggctgc tcgacgcggg
     2221 ctgcggcacc ggtgcgtcca ctgcggccct gctgtcggtg gccccgcagg ccgacatcgt
     2281 ggccgtcgac gcctcgtcgg gaatgctggc ggaagccgcc gccaaacact ggcccgcgtc
     2341 cgtgcggttc gtgcacagcc gcatcgagga catcgccgaa gccggtgtcg aggggccctt
     2401 cgacggcatc ttcgcggcgt acctgctgcg caacctcgag gatcccgacg ctcagttgcg
     2461 ggagtttctg gcgctgttgc ggccaggggc gacgcttgcg gtgcacgagt actcggtccg
     2521 cgactcccgg gtcgcggcgg tgatgtggaa cgcggtatgc gcgacgatca tcatcccgat
     2581 gggcaggctg cgcagcgggg atgccggcct ctaccgctac ctacggcgca gcgtcaacga
     2641 atttgacggc gcggaaacct tcctgaaccg gctgcgggac aacggcttca caagtgcgcg
     2701 tagcgagacg gtgccgggtt ggcagcgcaa catcgtgcac acattcctcg cagaagcgcc
     2761 cgcatgaccg acccccggcg cgccagtcat cccgcggcgc ccggtctgcc cgatgccggt
     2821 tcgttgggca gcacgccgca cgtcgtcgtg gtgggcggcg gcatcgccgg gctggcggcg
     2881 gctaccgggc tgatcgagcg cggcgtgacc gtcgacgtc
//
LOCUS       DASYLX010000214         7975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3629390,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000214 DASYLX010000000
VERSION     DASYLX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629390"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..916
                     /gene="prmC"
                     /locus_tag="VGP27_05130"
     CDS             53..916
                     /gene="prmC"
                     /locus_tag="VGP27_05130"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV7850735.1"
                     /translation="MTATARLRREIEHAAAALAEAGVGSPRADAEELAAYAAGTSRGL
                     LPMLGDVDEQFCGRYADLVAARASRIPLQHIVGSAALGPVTVSVGPGVFIPRPETEGL
                     LEWASAQPLPPRPVLVDLCTGSAALALALAKFWPDARIIAVDDSDDALAYARRNVSGT
                     TVDLVRADVTDISLLPELNRKVDLLVANPPYIPDGATLEPEVAQHDPSHALFGGADGM
                     VVIDAIVELACRWLKPGGGCAVEHDDTTSAATVAAFTAAGDFVDVTPHRDLTGRPRFV
                     TATREKVEANG"
     gene            909..1568
                     /locus_tag="VGP27_05135"
     CDS             909..1568
                     /locus_tag="VGP27_05135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV7850736.1"
                     /translation="MAELFDCADADSRATGIASAVSAVRGGRLVVLPTDTVYGIGADA
                     FDSTAVNALLSAKGRGRDMPVPVLVGSWHTIEGLVYTVPDAARELIRAFWPGALSLVV
                     QQAPSLQWDLGDAAGTVMLRMPLHPVAIEVLREVGPMAVSSANISGRPAAVTAEGARN
                     QLGDLVAVYLDAGPSEQQAASTIVDLTGADPRILRAGPVTAEEIAKVLAVEAESLTAT
                     T"
     gene            1696..2898
                     /locus_tag="VGP27_05140"
     CDS             1696..2898
                     /locus_tag="VGP27_05140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV7850737.1"
                     /translation="MVSDVHNVAGGLLALSDRGAGVPLRELALVGLTAAIITYFATGW
                     VRVLATRVGAVAYPRERDVHLQPTPRMGGLAMYIGVLGAVLLASQLPALTRGFVYSSG
                     LPATVVAGGLIMAIGLIDDKWGLDALTKFAGQITAASVLVTMGVAWNVLYIPIGGVGT
                     IVLDQVSSILLTLALTVAIVNAMNFVDGLDGLAAGLGLITALAITIFSIGLLRDHGGD
                     VLFYPPAVISVVLAGACLGFLPHNFHRARIFMGDSGSMLIGLMLGAAATTAAGPISQS
                     AYGARDVFALLSPFLLVVAVMFVPALDMLLAIVRRTRAGRSPFSPDKMHLHHRLLQIG
                     HSHRRVVLLIYLWVGIVALGAASTIFFDPRYTGGVMLAAILVAIVVTLIPLLRRREES
                     YDSMYDEK"
     gene            3136..3609
                     /locus_tag="VGP27_05145"
     CDS             3136..3609
                     /locus_tag="VGP27_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEV7850738.1"
                     /translation="MTTPAQDAPLVFPSVAFRPLRLFAVCVVFAGLVTVAAALLDHPM
                     VGVFFGVGLALGLLNAILVQRSVEAITAKDHPLKSKMAINSATRLLVMTVIGLTIAFI
                     FRPQGLGVVFGMALFQVLLVLSTALPVLKKLRAGDADARSTGVGSDGLEGEAGEK"
     gene            3606..4394
                     /gene="atpB"
                     /locus_tag="VGP27_05150"
     CDS             3606..4394
                     /gene="atpB"
                     /locus_tag="VGP27_05150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV7850739.1"
                     /translation="MTGFDVADPAAPVTILAAESGIEVGHHTQAHWFGLTVNTDTVLS
                     TAIAAAIVLALAFFLRAKVTSTGVPSGVQLFWEAITVQLRNQIESAIGMKIAPFVLPL
                     AVTLFVFILVSNWLSILPVQYTDSNGGIHELLKPPASDINFVLALALFVFICYHAAGI
                     WRRGIIGHPVKLLKGHVAVLAPINLVEELAKPISLSLRLFGNIFAGGILVALIAMFPP
                     WIMWAPNAIWKSFDLFVGAIQAFIFALLTILYFSQSMELDEQHD"
     gene            4456..4701
                     /locus_tag="VGP27_05155"
     CDS             4456..4701
                     /locus_tag="VGP27_05155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV7850740.1"
                     /translation="MNPTIAAGALIGGGLIMAGGAIGAGIGDGIAGNALIAGIARQPE
                     AQGRLFTPFFITVGLVEAAYFINLAFMALFVFATPVK"
     gene            4715..5236
                     /locus_tag="VGP27_05160"
     CDS             4715..5236
                     /locus_tag="VGP27_05160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV7850741.1"
                     /translation="MNDLSSTILAAEEGGGQSNFLIPNGTFFFVLIIFLILLGVIAKW
                     VVPPISKVLREREAMVHKTAEDNRRAAQLRAAADADYLKVMATARREATHVREEARAE
                     GRKIVEDARARANAEVSKVLAQATEELTEQSLAVTADLQTSVETLAAQLASRVLGVQV
                     TTRTVPVTTGQGR"
     gene            5242..6573
                     /locus_tag="VGP27_05165"
     CDS             5242..6573
                     /locus_tag="VGP27_05165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B/delta"
                     /protein_id="HEV7850742.1"
                     /translation="MSTFIGQLIGFAVIVFLLWKYVVPPVRTMMKNQQEIVRAQLQEH
                     SEAEKKVADADKEHAKALEQAKAEASKVIEEARHDAEKIAEQLRAQADAELERIKTQG
                     AQQVQLLRQQLIRELRQTLGAESVHRARDMVRDFVSDPAEQSATVGRFLDELDEMAPS
                     STVFSDAATAKLRAASRQSLATVVEQFDEAVAGLDGDGLTKLSDDLASTVKLLRREPV
                     LARHLADPSAGSASRVHLVERLLSGKVSESALDILKNAVSQRWSSASDLTRGVQHVAR
                     LALLVRAEADGQIEDVEEELFRFSRILDTEPRLITLLSDYTSPLDGRISLLNNVLHRR
                     ASRYTADLLRQTIELLHGERADDAVRELANLAVSRRGEVVAQVRAASELSDAQRNRLT
                     ELLTRIYGHPVSLQLDIDPALLGGLTIEVGDEVIDGSLSSKLASAENHLPE"
     gene            6653..>7975
                     /gene="atpA"
                     /locus_tag="VGP27_05170"
     CDS             6653..>7975
                     /gene="atpA"
                     /locus_tag="VGP27_05170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV7850743.1"
                     /translation="MAELTISADEIQGAIEEYVAGFEADTEREEIGTVIDAGDGIAHV
                     EGLPSVMTQELLEFPGGVLGVALNLDEHDIGAVILGDFEKIEQGQQVKRTGEVLSVPV
                     GDAFLGRVVNPLGQPIDGRGDVETDERRALELQAPSVVQRQGVSEPLQTGIKAIDSQT
                     PIGRGQRQLIIGDRKTGKTAVCVDTILNQRQNWETGDPDQQVRCVYVAIGQKGTTIAS
                     VRQTLEEGGAMEYTTIVASPASDSAGFKWLAPYTGSSIAQHWMYDGKHVLIVFDDLTK
                     QAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSLTGLPIIETKA
                     NDISAYIPTNVISITDGQCFLETDLFNQGVRPAINVGVSVSRVGGAAQIKAMKEVAGS
                     LRLDLSQYRELESFAAFASDLDETSKAQLDRGARLVELLKQPQNSPMPV"
BASE COUNT         1333 a         2603 c         2606 g         1433 t
ORIGIN      
        1 ccgccgacaa gcaatcgcgg ctgcagcagg catgagcccg ctcggcaacc gcatgacggc
       61 gaccgcgcgg ctgcgacgag agatcgagca cgcggccgcc gcgctcgccg aggcgggtgt
      121 cggatctccg cgggccgacg ccgaggagct ggccgcctac gccgcaggga caagccgcgg
      181 cctgctgccg atgctcggcg acgtggacga gcagttctgc ggtcgctacg ccgatctcgt
      241 cgccgcccgc gccagccgaa tcccgttgca gcacatcgtc ggctccgcag cgctgggtcc
      301 ggtgaccgtt tcggtggggc ccggcgtgtt catcccgcgg cccgagacgg agggcctgtt
      361 ggaatgggcc tcggcgcagc ccttgccgcc gcgaccggtg ctggtcgacc tgtgcaccgg
      421 ctcggccgcg ctggcgctgg cgctggcgaa gttctggccc gacgcgcgca tcatcgccgt
      481 cgacgactcc gatgacgcgc tggcctatgc gcgccgaaac gtctctggca cgacggttga
      541 cctcgtgcgg gccgacgtca ccgacatctc gctgttgccg gagttaaatc gaaaggtcga
      601 tctcctggtc gccaacccgc cctacattcc cgacggcgcc actctggaac ctgaagtggc
      661 gcagcatgat ccgtcacatg ctttgtttgg cggggcggac gggatggtgg tcatcgacgc
      721 gatcgtcgaa ttggcgtgcc gctggctgaa gcccggcggc gggtgtgcgg tcgagcatga
      781 cgacaccacg tcggccgcca ccgttgcagc gttcaccgcg gccggcgatt tcgtcgacgt
      841 gacccctcac cgcgacctga caggccgccc ccgctttgtc accgcaactc gtgagaaggt
      901 ggaggcaaat ggctgaattg ttcgactgcg ccgatgccga ctcgcgcgca accggaatcg
      961 cttcggccgt cagcgcggtc aggggtggcc gcctcgtcgt gctgcccacc gacaccgtct
     1021 acggcatcgg cgccgacgca ttcgacagca ccgcggtgaa tgcgctgcta tcggccaagg
     1081 gccgtggtcg cgacatgccc gtgccggtgc tcgtcggctc ctggcacacc atcgagggtc
     1141 tggtgtacac ggtgcccgac gccgcgcgcg agctgatccg tgcgttctgg cccggtgcac
     1201 tgagcctcgt tgtgcagcag gcgccgtccc tgcaatggga cctcggggat gccgccggca
     1261 cggtgatgct gcggatgccg ctgcacccgg tcgcgatcga ggttctgcgc gaagtcggcc
     1321 cgatggcggt gtccagcgcg aacatctcgg gccggcccgc cgcggtcacc gcggaagggg
     1381 ctcgcaatca gctcggcgat ctcgtcgcgg tctacctcga cgccggaccg tccgaacagc
     1441 aggcggcttc cacgatcgtc gacctcaccg gcgcagatcc gcgcatcctt cgggccggtc
     1501 cggtgaccgc cgaggagatc gcgaaagtgc ttgcagtcga agccgaatcg ctgacggcga
     1561 ctacctgagc cgcacccgga gcatggagac acgcagtcgg gacggcacgc ggaatgccgg
     1621 cgatcgtcgc gggccgctct cggacaggtc gttcacagca gaggcttctc aacctcgtcc
     1681 agtacggttc attcagtggt gagcgatgtg cacaatgtgg ccggtgggct gcttgcgctc
     1741 tccgatcgag gcgcgggcgt gccgctacgc gagctggctc tggtcggtct caccgcggcg
     1801 atcatcacct atttcgcgac gggctgggtc cgcgtgctgg cgacgcgcgt cggcgccgtg
     1861 gcttacccca gggaacgcga cgtccatctg cagccgacgc cgcggatggg cgggctggcg
     1921 atgtacatcg gggtgctggg cgccgtgctg ctcgcctccc aactgcccgc gctgacccgc
     1981 ggcttcgtct actcatcggg tctgcccgcc accgtcgtgg ccggtgggct gatcatggcc
     2041 atcggcctga tcgacgacaa gtggggcctg gacgcgctga ccaagttcgc cgggcagatc
     2101 accgcggcca gcgtgctcgt cacgatgggc gtggcgtgga acgtgctata catcccgatc
     2161 gggggtgtcg gcaccatcgt gctcgaccag gtgtcgtcga ttctgctgac gctggcgctg
     2221 acggtggcga tcgtcaacgc catgaacttc gtcgacggcc tcgacgggct ggccgcgggt
     2281 cttggcctga tcaccgcgtt ggccatcacc attttctcca tagggctgct tcgcgaccac
     2341 ggcggcgacg tgctgttcta cccgcccgcg gtgatttcgg tggtgctggc aggcgcctgt
     2401 ctgggttttc tgccgcacaa tttccatcgc gcgcgcatct tcatggggga ctccggttcg
     2461 atgctgatcg ggctgatgct cggggcggcc gccaccacgg ccgccgggcc gatctcgcag
     2521 agcgcttacg gcgcccgcga cgttttcgcg ctgctgtcac cgttcctgtt ggtggttgcc
     2581 gtgatgttcg tgcccgccct ggacatgttg ctggccatcg tccggcgcac ccgagccgga
     2641 cgcagtccgt tcagccccga caagatgcac ctgcatcacc ggctgctgca gatcggacat
     2701 tcgcaccgac gggtggtgct gctcatctac ctgtgggtgg gaatcgtcgc gctgggcgcc
     2761 gcgagcacga tcttcttcga cccccgctac accggcggag tgatgctggc ggcgattctg
     2821 gtggccatcg tcgtcacgct cattccgctc ctcagacgcc gggaagagtc ctacgacagc
     2881 atgtacgacg aaaagtagta gtacccggtt tctggctgct tcctgtgtgg tacggtgctg
     2941 gtagaacccg aaaaacctcg cgggttgccg gcccccgagc cgcccggctc accaccgacc
     3001 ggcacggata aacgaccgac aaagggagct accccttggg tgattccagc gtgtcctgcg
     3061 cactccgata cgctcggggc gccacgcaca gaatcattgg gttagccctc gtgaccgcgg
     3121 gattgaggtg acgccgtgac gacgccagcg caggatgcgc cgttggtgtt tccgtcagtg
     3181 gctttccggc cgttgcggtt gtttgccgtg tgtgtggttt tcgccggcct ggtgacagtg
     3241 gcggccgcgc tgcttgacca tccgatggtc ggggttttct tcggagtggg cctcgcgctc
     3301 ggtttgctca acgcgatcct cgtgcagcga tcggtcgagg cgatcacagc caaggatcat
     3361 ccgctcaaga gcaaaatggc aatcaactcc gccacccggc tgttggtgat gacggtgatc
     3421 ggcttgacga tcgcattcat cttccggccg cagggtctcg gcgtcgtgtt tggcatggcg
     3481 ctgttccagg tattgctagt gctttccacg gcgctgcccg tcttgaagaa gctgcgggcg
     3541 ggcgacgcgg acgcgaggag caccggcgtc ggtagtgatg gactcgaagg ggaggcagga
     3601 gagaaatgac cggatttgat gtcgccgacc cagcggctcc ggtgacgatc ctcgccgccg
     3661 aatccgggat cgaagtcggc caccacacgc aggcgcactg gttcgggctg accgtcaaca
     3721 ccgacacggt gctgtccacc gcgattgccg cggcgattgt gctggcgctc gcattcttcc
     3781 tgcgcgccaa ggtgacgtcg accggagtgc ccagcggcgt gcagttgttc tgggaagcga
     3841 tcacagttca gctgcgcaac cagatcgagt cggcgatcgg catgaagatc gccccgttcg
     3901 tgctgccgct ggccgtcacg ctgttcgtgt tcattctggt gtcgaactgg ctgtcgatcc
     3961 tgcccgtgca gtacaccgat tccaacggcg ggatacacga actgctcaag ccgccggcct
     4021 cggacatcaa cttcgtgctg gcgctcgcac tgttcgtgtt catctgctac cacgcggcgg
     4081 gcatttggcg ccgcggcatc atcggccacc cggtgaagct actgaagggc cacgtcgccg
     4141 ttctggcgcc gatcaatctc gtcgaggagc tcgccaagcc gatctcgctg tcactccgac
     4201 ttttcggcaa catcttcgcg ggcggcatcc tggtcgcgct gatcgccatg ttcccgccgt
     4261 ggatcatgtg ggcgcccaac gcaatctgga agagcttcga cttgttcgtc ggcgccatcc
     4321 aggcgttcat cttcgccctg ctgacgatct tgtacttcag ccagtcgatg gaactcgacg
     4381 aacaacatga ctgataccga ccatgctcga cccacgtcct ggtaggacac ctgccagtta
     4441 tcaaggagga taaagatgaa tcccacaatc gctgccggcg cgctcatcgg cggtggactg
     4501 atcatggccg gcggtgccat cggcgccggt atcggtgacg gcatcgcggg caacgcgttg
     4561 atcgccggca tcgcccggca gcccgaggcc cagggacgct tgttcactcc gttcttcatc
     4621 acggtcggtc tggtcgaggc cgcctacttc atcaacctgg cattcatggc gctgttcgtg
     4681 ttcgccacac cggtcaagta ggtagtccac ggtcatgaat gacctcagct cgacgatcct
     4741 ggcggcggaa gaaggcggcg gtcagagcaa ctttctgatc cccaacggca cgttcttctt
     4801 cgtgctgatc atcttcttga tcctgctcgg tgtgatcgcc aagtgggttg tgccgccaat
     4861 ctccaaggtt ttgcgcgaac gcgaagccat ggtgcacaag acggctgagg acaaccgtcg
     4921 ggcagcgcaa ctgcgggcgg ctgctgacgc cgactacctg aaggtgatgg ccacggcccg
     4981 ccgcgaggcg acgcacgtcc gtgaggaggc ccgtgccgag ggccgcaaga tcgtcgagga
     5041 cgcgcgcgcc cgtgccaacg ccgaggtctc gaaggtgctt gcgcaggcca ccgaggagct
     5101 cacggagcag tcgctagcgg tgacggccga cctccagacg tctgttgaga ctctggcggc
     5161 gcagcttgcc agtcgcgtcc tcggggtcca ggtgaccact cgtacggttc cggttactac
     5221 aggacagggg cgttagctgt cgtgtcgacc ttcatcggac agctcattgg tttcgcggta
     5281 atcgtcttcc tcttatggaa atacgttgta ccgccggttc gcaccatgat gaagaaccag
     5341 caggagatcg tccgcgcaca gctgcaagaa cactccgagg ccgagaaaaa ggtcgccgac
     5401 gccgacaagg agcacgccaa ggcgctcgag caggccaagg ctgaggcgtc gaaggtgatc
     5461 gaggaggcgc gtcacgacgc cgagaagatc gccgaacagc tgcgggccca agccgacgcc
     5521 gagctggagc gcatcaaaac gcaaggggca caacaagttc agctgcttcg tcagcagctc
     5581 atccgcgaac ttcggcagac tctcggcgcc gagtccgtgc atcgtgcaag agatatggtg
     5641 cgcgacttcg tctccgatcc agcagagcag tccgcgacgg ttggccgctt cctcgacgag
     5701 ttggatgaga tggcgccgtc gagcacggtc ttctccgacg ccgccacagc gaagctgcgc
     5761 gccgccagtc gccagtcact ggccacagtc gtcgagcagt tcgacgaggc cgtggccggg
     5821 ctcgacggcg acggactgac caagttgtcc gacgatcttg catcgacggt caagttactg
     5881 cgccgcgagc cggttttggc caggcatctg gccgacccct ccgcgggttc cgcttccagg
     5941 gtgcacctcg ttgagcgact gctgtccggc aaggtttccg aatccgcact cgacatactc
     6001 aaaaatgccg tttcgcagcg ctggtcgtcc gcctcggact tgacgcgcgg agtccaacac
     6061 gtcgccaggc tggccctgct ggtgcgcgct gaagccgacg gacagatcga agatgtggag
     6121 gaggagctgt tcaggttcag tcgcatcctc gacaccgagc cgcggctgat caccctgctc
     6181 agcgactaca cctcccccct ggacggccga atcagtctgt tgaacaacgt gcttcaccgc
     6241 agggcgagca ggtacaccgc ggacttgctt cgccagacga tcgaactgct gcacggcgag
     6301 cgtgctgatg acgccgtccg cgagttggcg aatctcgcgg tgtcccgacg tggcgaggtc
     6361 gtcgcgcagg tgcgcgccgc aagcgagctc agcgacgctc aacgaaaccg actgaccgag
     6421 ttgctcaccc gcatctacgg tcatccggtg tcgcttcagc tcgacatcga cccggcactg
     6481 ctcggcggcc tcacgatcga ggtgggcgac gaggtgatcg acggctcgct gtcgtccaag
     6541 ctggcgtcgg ccgaaaacca cttgcccgaa tgaccccgac aacaccagac ccacacacga
     6601 agtaggaaga cgaaaagcca ccgatgagcg cttgcgcgaa gaggagaacc ccatggcaga
     6661 gttgacgatc tcggcggacg agatccaggg cgccatcgag gagtatgtcg ccggtttcga
     6721 agccgacacc gagcgcgagg aaatcggcac cgtcatcgac gccggcgacg gcatcgcgca
     6781 cgtcgagggc ctcccgtcgg tgatgacgca ggagctgctg gagttccccg gcggtgtgct
     6841 cggtgttgcg ctcaacctcg acgagcacga catcggcgcg gtgatcctcg gcgacttcga
     6901 gaagatcgaa cagggccagc aggtcaagcg caccggcgag gtgctgtcgg tacccgttgg
     6961 tgatgccttc ctcggacgtg tggtcaaccc cctgggtcag ccgatcgacg gtcgcggcga
     7021 tgtcgaaacc gacgagcgac gcgccctgga actgcaggcg ccgtctgtgg tgcagcgtca
     7081 aggcgtcagt gagccgcttc agacgggcat caaggcgatc gactcccaga ccccgatcgg
     7141 tcgcggccag cgccagctga tcatcggcga ccgcaagacc ggcaagaccg cggtatgtgt
     7201 ggacacgatc ctcaaccagc gccagaactg ggagaccggc gaccccgacc agcaggtccg
     7261 ttgcgtgtac gtggcgatcg gccagaaggg caccaccatc gccagcgtgc gccaaaccct
     7321 cgaagagggt ggcgcgatgg agtacaccac gatcgtcgct tcaccggcct ccgactcggc
     7381 cggcttcaaa tggcttgcgc cgtacaccgg gtcgtccatt gcccagcact ggatgtacga
     7441 cggcaagcat gtgctgatcg tgttcgacga cctgaccaag caggcggagg cctaccgtgc
     7501 catctcgctg ctgctgcgca ggccgcccgg ccgcgaggcg tacccgggcg acgtgttcta
     7561 cctgcattcc cggctgctgg aacgttgtgc caagttgtcc gacgagctcg ggggcggctc
     7621 gctgacgggc ctgccgatca tcgagacaaa ggccaatgac atctcggcct acatcccgac
     7681 caacgtcatc tcgatcaccg acggccagtg cttcctggag accgatctgt tcaaccaggg
     7741 tgtgcgcccg gcgatcaacg tcggcgtttc ggtgtcgcgt gtcggcggtg ccgctcagat
     7801 caaggccatg aaagaggttg ccgggtcgct gcggctggat ctctcgcagt atcgcgagct
     7861 cgaatcgttc gccgcgttcg cttccgacct cgacgagaca tccaaggcgc agctggatcg
     7921 cggcgctcgg ctggtggaat tgctcaagca gccgcagaac agcccgatgc cggtc
//
LOCUS       DASYLX010000215         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1655296,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000215 DASYLX010000000
VERSION     DASYLX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655296"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1030
                     /locus_tag="VGP27_05175"
     CDS             32..1030
                     /locus_tag="VGP27_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7850744.1"
                     /translation="MSACAKKRQQLMSIRMLCVAVAMVTVLAGCGRAEEVPPTPNLTL
                     PAPTPAGMQELPPSLAPVPEDTDNECDVTASLRPFSDRAQADDAVATIKNRGRLIVGL
                     DIGSNLFSFRDPITGEITGFDVDIAGEVARDIFGTPSQVEYRILSSADRITALQNNQV
                     DIVVKTMSITCERKRLVNFSTVYLVANQRILAPRDSTISQAGDLSGKRVCAVKGTTSL
                     QRIQQINPSPIVIGVVTWADCLVALQQRQVDAVSTDDSILAGLVAQDPYLRIVGPSMN
                     EEPYGIGINRENTGLVRFVNGTLERIRRDGTWSTLYRKWLTVLGPPPAPPVPRYSD"
     gene            1030..>1815
                     /locus_tag="VGP27_05180"
     CDS             1030..>1815
                     /locus_tag="VGP27_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV7850745.1"
                     /translation="MSTAEEGFEGRAKPQAEPTVGTQPASLEDLEELDTDSASTIRPM
                     ATQAVFRPHFDDSDGTSVGTADTEPEDHTTVMTRALSPTRRLGGGLVEIPRVREIDPL
                     SALMTNPVVAEGKRFCWNCGKAVGRSSSDGRALSEGWCPHCGSAYSFLPQLVPGDMVA
                     EQYEIKGCIAHGGLGWVYLAFDHNVNGRPVVLKGLVHSGDAEAQKIAMAERQFLAEVT
                     HPGIVKIYNFVEHPDQHGNPVGYIVMEYVGGKSLKQARGDKLPV"
BASE COUNT          327 a          604 c          609 g          275 t
ORIGIN      
        1 gactgtggcc ccgacttagc gagtaccgct gatgagcgct tgcgcgaaga agagacagca
       61 gctgatgagc atcaggatgt tgtgcgtcgc ggtcgccatg gtcaccgtgc tggcgggttg
      121 cggccgcgcg gaagaggtgc ccccgacgcc gaacctcacg ctgcccgcgc ccacccccgc
      181 aggcatgcag gaactgccgc cgtcgctggc gccggtgccg gaggacaccg acaacgagtg
      241 cgacgtgacc gcgagcctgc ggccgttctc cgaccgcgcg caggccgacg acgccgtcgc
      301 cacgatcaag aaccgcggcc ggctcatcgt cggcctcgac atcggcagca acctgttcag
      361 cttccgcgac ccgatcaccg gggagatcac cggattcgat gtcgacatcg ccggcgaggt
      421 ggcccgcgac atcttcggca ccccgtcgca ggtcgagtac cgcatcctgt cgtcggccga
      481 ccgcatcacc gcgctgcaga acaaccaggt cgacatcgtc gtcaagacca tgagcatcac
      541 gtgcgagcgc aagcgtctgg tcaacttctc gacggtgtac ctggttgcga accaacgcat
      601 tctggcgccg cgcgactcga cgatctcgca ggccggtgat ctgtccggca aaagggtctg
      661 cgccgtcaag ggcaccacgt cgctgcagcg gatccagcag atcaacccgt cgccgatcgt
      721 catcggggtg gtgacgtggg ccgactgcct ggtggcgctg cagcagcgtc aggtcgacgc
      781 ggtcagcacc gacgactcca tcttggccgg gctggtggca caggacccgt atctgcgcat
      841 cgtcgggccc agcatgaacg aggagcccta cggaatcggc atcaaccggg agaacaccgg
      901 gctggtccgg ttcgtcaacg gcacgctgga gcgcatccgc cgcgacggca cgtggagcac
      961 gttgtaccgc aaatggctta cggtgctcgg cccgcctccc gctcccccgg ttccgaggta
     1021 ctcggactga tgtcaacggc agaggaagga ttcgaagggc gtgcgaagcc gcaggctgag
     1081 ccgacggttg ggacccaacc cgcgagcctg gaagacctcg aagaactcga cacggattcc
     1141 gcgtcgacga tccggccgat ggccacccag gccgtcttcc ggccgcactt cgacgattcg
     1201 gacggcacgt cggtcggcac cgccgacacc gagcccgagg accacacgac ggtgatgacg
     1261 cgggcgctgt cgccgacgcg caggctcggc ggcgggctgg tcgagatccc gcgggtgcgc
     1321 gagatcgacc ccctgtcggc cctgatgacc aatcccgttg tggccgaagg caaacggttc
     1381 tgctggaact gcggcaaggc ggtcggcagg tcgtcatccg acggcagagc gttgtcggag
     1441 ggttggtgcc cacactgcgg cagcgcgtat tcgttcctgc cgcagctggt tcccggcgac
     1501 atggtggccg agcagtacga gatcaagggc tgcatcgcgc acggcggtct gggctgggtg
     1561 tatctggcct tcgaccacaa cgtcaacgga cggccggtcg tgctcaaggg tctggtgcat
     1621 tccggtgacg cggaagccca gaagatcgcg atggccgagc ggcagttcct cgccgaggtg
     1681 acgcacccgg gcatcgtcaa gatctacaac ttcgtcgagc atcccgacca gcacggcaac
     1741 cccgtcggct acatcgtgat ggagtacgtc ggcggaaagt cgctcaagca ggccaggggc
     1801 gacaagctac cggtg
//
LOCUS       DASYLX010000216         7003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_667937,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000216 DASYLX010000000
VERSION     DASYLX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667937"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGP27_05185"
     CDS             complement(<1..460)
                     /locus_tag="VGP27_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEV7850746.1"
                     /translation="MADATSKPVDTERSRTLWFAALLTLTNGFLDAHTYIARGHVFAN
                     VQTANVIFSAIHVSERQFSAALAHLWPLLAFIAGMLLASHIKSGRMEQMLPHSLRWTL
                     GVQAFVLAIIGFVPASVDHNFVTVPISFLAAVQIGLFRNVGDLAYLPVATT"
     gene            complement(486..1592)
                     /locus_tag="VGP27_05190"
     CDS             complement(486..1592)
                     /locus_tag="VGP27_05190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7850747.1"
                     /translation="MRVVQVANFYGPRSGGLRTAVDRLGAEYAAAGHEVFLIVPGQHS
                     EQVELASGVTRISLPARLIPFTSGYRAVSPRPVRALLEDLEPDAIEVSDRLTLRSLGL
                     WGRQHDVATVMISHERLDRLVGQVLPRRVARSVADFANRRTAEAYDAVVCTTAFARAE
                     FDRIHAPNVLTVPLGVDLDQFHPRHHSATMRLTWAGREQLLLVHCGRLSVEKHAHRSI
                     DTVAALRHSGVDARLVVVGEGPLRARLERQAARLPVHFTGYIGCRDTVATILASADVA
                     LAPGPHETFGLAALEALACGTPAVVSRTSALAEILSTDSGATADNDPRAIADAVSSII
                     SRPEALRRTNARRRAEQFTWPRAAAGMLGALGGV"
     gene            complement(1710..2375)
                     /locus_tag="VGP27_05195"
     CDS             complement(1710..2375)
                     /locus_tag="VGP27_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850748.1"
                     /translation="MTVQQRLTAGLAAGMLAAGAAVASAATAAADDIPPPPAPTEPAP
                     PPAAPAPAGPTGFLPPAGTLQNFLPEGGMAPGSGYDFLLGQEPIPTAQGGQPTPTVVG
                     EDGQPVPAAPQQTILDPSAIQAMKPTNFSLAGQGQQSMYSYTPAAPGAPPPTFIDNAR
                     GAHGIWNYGMGRLTTEQLGQPLPGTAPPPGTNIPAGLAPDPNDPSAPPPPQPPFPFTA
                     ATP"
     gene            complement(2415..3092)
                     /locus_tag="VGP27_05200"
     CDS             complement(2415..3092)
                     /locus_tag="VGP27_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEV7850749.1"
                     /translation="MLNRFATVAAVCMLVPAGPAAVALADDPPPAIPAGDVGPPPDNG
                     VVPSGPPGIAPASEGRTLTVVAKDETQLPVAPLTTSLASRDWLVGATFTGSGMGSAKG
                     GSLEVGYQIGCAIEMDKIKLNGSIGIGSNAASLSGAGAIGLPTTLSFPIQGQIEVEPR
                     PGTITNVAVDKKSYKGNSTRVTVKDIHIKVDNCVGASSLRSYAILTSSNGDTDDVVAY
                     YGVTKVF"
     gene            complement(3540..4067)
                     /locus_tag="VGP27_05205"
     CDS             complement(3540..4067)
                     /locus_tag="VGP27_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEV7850750.1"
                     /translation="MKTPDGYTLTVQGQNESMEPVAALTGAPTSREYLVDGQFTGSVT
                     GGGGPKLAGGSLEAGYQIGCGIAQDDIESIISAGINPGVGIPFTNGSIFPITITPSLA
                     YQMKIDLKPGTVNIVPVDKKTFKGTSPKVNITGLRVKFDQCAGSSFIRSYATLTSSTD
                     DTDDVVTYIGVTKMV"
     gene            4582..5223
                     /locus_tag="VGP27_05210"
     CDS             4582..5223
                     /locus_tag="VGP27_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850751.1"
                     /translation="MRSSYVYGRTAATTLMVFGIGLAIVPAASADPGGADQTQPPAPA
                     PAALPDAPAPGAVPEAAAMAGAPIGPTGAPQGVAPDTVPMQTSGDPTRDACDLFNKAV
                     NYAAINYEDFADYSAGSGNYVNYQDQTVDNANAAGRTALRQAAAAALNASGIPGAAPE
                     ATAPMRSWSLSAAKLVLVMGVRGGGDTLNSAATDLNKTAKEAQMACATAQQQS"
     gene            complement(5521..5928)
                     /locus_tag="VGP27_05215"
     CDS             complement(5521..5928)
                     /locus_tag="VGP27_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850752.1"
                     /translation="MAGMATAAAALAMTVGTGTAQAKPNDGFNNIFHSNKSDSGSANK
                     PASGSTPTATVNAQTTAARNLSSSSTNIGSVLSSIAPSLYNPGPFTVGPVTSTPTGVI
                     VKPPVPGTKPFAQVTGIQLPFWTVNNGTPVPPA"
     gene            complement(6361..>7003)
                     /locus_tag="VGP27_05220"
     CDS             complement(6361..>7003)
                     /locus_tag="VGP27_05220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7850753.1"
                     /translation="GLQGAWAEAPDARGGVLVIHENKGLNDWVRSVAGRFAGIGYSAL
                     AIDLLSAQGGTAKFSDPAEATAALGNIPNEQFVANLKSGVGELQRRVPDKKIAAVGFC
                     MGGGLVWQVLAAGTPELAAAVPFYGPAPDNPDFAGSKNAAVLAFYGALDDRVTKTEPV
                     VRAALEKAGLVHELITEPDANHAFFNDTGDRYNATAAADAWRRVQDWFGKYVV"
BASE COUNT         1248 a         2207 c         2292 g         1256 t
ORIGIN      
        1 cggtggtggc gacgggcagg tacgccaggt caccgacgtt gcggaacaga ccgatctgca
       61 ccgcggccag aaacgagatc ggcaccgtca cgaagttgtg gtccaccgag gcgggcacga
      121 agccgatgat cgccaggacg aaggcctgaa ccccgagggt ccagcgcagc gagtgtggca
      181 gcatctgctc catgcgaccc gacttgatgt gggatgccag cagcatgccg gcgatgaacg
      241 ccagcagcgg ccacagatgc gccaacgcag cggagaattg ccgctccgac acatggatcg
      301 ccgaaaagat gacattggcc gtctgcacgt tggcgaacac gtggccgcgg gcgatgtagg
      361 tgtgagcgtc gaggaacccg ttcgtcagcg tcagcaatgc cgcaaaccac agcgtcctgg
      421 accgttcggt gtccaccggt ttgcttgtcg cgtccgccac tgggcaaaag taacgcagtg
      481 cgcggctata ccccgcccaa tgctcccagc atgccggccg ccgcgcgggg ccaggtgaac
      541 tgctcggcgc gccgccgtgc attcgtgcgt cgcagtgcct ccggcctgct gatgatcgat
      601 gacacggcgt cggcgatcgc tcgcgggtcg ttgtcggcgg tcgcgccgct gtcggtggaa
      661 agtatctcgg ccagcgccga ggttctcgac acgacggcgg gtgtgccgca ggccagtgcc
      721 tccagcgcgg cgagcccgaa cgtctcgtgc ggaccaggcg caagcgccac gtcggccgac
      781 gccagaatcg tcgccaccgt gtcgcggcac ccgatatagc cggtgaagtg aaccggcaac
      841 cgggcggcct gacgctccaa tcgcgcccgc agcggtccct ccccgaccac gacgagtcgc
      901 gcatcgacgc ccgaatgccg aagcgcagca acggtgtcga tgcttcgatg cgcatgcttc
      961 tccaccgaga gacggccgca gtgcacgagc agaagctgct cgcggccggc ccacgtcagc
     1021 cgcatggtcg ccgaatggtg gcggggatgg aattggtcga ggtcgacgcc gagcggcacc
     1081 gtgagcacat tcggcgcatg gatccggtcg aactcggcgc gggcgaacgc ggttgtgcag
     1141 accacggcgt cgtaagcttc ggccgttctg cggttggcga agtcggcgac gctacgggcc
     1201 acccgacgcg gaagaacttg tccgacgagg cggtcaaggc gttcgtgcga gatcatcacg
     1261 gtcgcgacgt cgtgttgacg cccccaaagg ccgagtgacc gcaaggtcag tcgatccgac
     1321 acctcgatgg cgtccggctc caggtcttcc agcagtgccc tgaccggccg tggcgataca
     1381 gcgcggtagc cgctggtgaa tggaatcagc ctggccggca acgaaattcg ggtgacaccg
     1441 gatgccaatt cgacctgttc ggagtgttgg cccgggacga tcaggaacac ctcgtggccc
     1501 gcggcggcgt actccgcgcc caggcggtcc acagccgttc tcaggccacc ggagcggggg
     1561 ccgtagaaat tggcgacctg aactacgcgc atgacttgaa tgagaactgg cacgtctgtg
     1621 ccatcagcga cgcctctgcg actggtgtct taacgacccg tgaatagctt tgtgcgtgca
     1681 ggacgttcgg gagcccgtca gtgtgccggt tacggcgtcg ctgcggtgaa gggaaacggt
     1741 ggctgcgggg gcggaggagc cgacggatca ttcgggtccg gcgccagacc agccggaatg
     1801 ttggttcccg gcggcggagc ggtacccgga agcggctgac cgagctgttc cgtggtcaac
     1861 ctccccatcc cgtagttcca gatgccgtgt gcgcccctgg cgttatcgat gaaggtagga
     1921 ggcggtgcgc cgggagcggc tggcgtgtag ctgtacatcg actgctgacc ctgcccggca
     1981 agcgagaagt tggtcggctt catggcttgg atagctgagg gatccagaat cgtctgctgg
     2041 ggcgccgccg gcacaggctg cccgtcctca cccaccaccg tcggcgtcgg ttgccctccc
     2101 tgcgctgtcg gaattggttc ttgaccgagg aggaagtcgt atcccgaccc cggcgccatt
     2161 cctccctctg ggaggaaatt ctgcaaggtc ccagcaggcg gcagaaaccc cgtcggcccc
     2221 gccggagcgg gcgctgcggg tggcggcgcg ggctcggtgg gtgccggtgg agggggaatg
     2281 tcgtcggcgg cggcggtcgc cgcagaggcg accgccgcgc cggcggccag catgcccgcc
     2341 gccaagccgg cggtgaggcg ctgctgaacc gtcacacggt caggacggat cacatgggga
     2401 cgtatcatcc tgactcagaa cacctttgtg acgccgtagt aagcgacgac gtcgtcggtg
     2461 tcaccattcg agctggtgag aatcgcgtac gaccgcagag acgacgcgcc gacgcagttg
     2521 tcgaccttaa tgtgaatgtc cttgacggtg acgcgggtcg agttgccctt gtaggacttc
     2581 ttgtcgaccg cgacgttcgt gatcgtgccg ggcctgggct cgacctcgat ctgaccctgg
     2641 atcgggaagc tcagcgtggt cgggagaccg atggcgccag cgccgctcag ggaagcggcg
     2701 ttagacccga tgccgatgct gccgttgagc ttgatcttgt ccatctcgat ggcgcaaccg
     2761 atctggtatc cgacttcgag tgacccgccc ttcgccgaac ccataccgga cccggtgaag
     2821 gtggcaccca ccagccagtc gcgcgatgcc agcgaggtcg ttaacggcgc caccggaagc
     2881 tgcgtctcat ccttcgcgac aacagtcagc gtgcgccctt cggaggcggg cgcaattccg
     2941 ggcggcccgg atgggacgac gccgttgtcc ggcgggggtc caacgtcgcc ggccgggatt
     3001 gccggaggcg gatcgtccgc caaggcgacc gcagccggtc cggccgggac cagcatgcaa
     3061 acagcagcca ctgttgcaaa acgattcaac atgtcgtgtt ggcgcctctc catcgttcga
     3121 ttccccaaag gatctgtcct gaggaatctt taagcgagcg agtgtccaaa cggtgaatgt
     3181 ggcaccaaca actcacgccc ggttcccgac cactgagcgt ctgcatgggc ggtgccgtcg
     3241 tgcgcaaccc ggtgccctta gcacactggc aaaagaagcg cctgtcgagc gacgctcaat
     3301 catctaactg ttttgagtgc tcgtcggtct aggacgacga aggctttgag cgccaatgaa
     3361 agacgatgct gtcttaactc attcagtttt agtgcgtctc attcgccggc ttgcttcagg
     3421 cagacaacga ctacggcttg aaacggcgca tgccccccac caccggtgag gggcatgcgg
     3481 tgaaaacgtg agccgtctcg gctacgccga aagacacccg tagatcgaat tcggaggcgt
     3541 tacaccatct tcgtgacgcc gatgtaggtc acgacgtcat cggtgtcatc cgtcgagctg
     3601 gtcagcgtcg cgtaggaccg gatgaacgac gaaccggcgc actgatcgaa cttgacacgt
     3661 agacctgtga tgttgacttt cgggctcgtg cccttgaagg tcttcttgtc caccgggacg
     3721 atgttgaccg tgcccggctt gaggtcaatc ttcatctgat aggccaacga aggcgtgatg
     3781 gtgatcggga agatgctgcc attggtgaac ggaataccca cacccgggtt gatgcccgcg
     3841 gaaatgatgc tctctatgtc gtcctgggcg atgccacagc cgatctggta accggcctcc
     3901 aacgatccgc ccgcgagctt cgggccgccc ccgccggtca cagatccggt gaactgcccg
     3961 tccaccagat actcgcggga ggtcggggcg ccggtgagcg ccgcgacagg ttccatcgac
     4021 tcgttttgac cctgcaccgt caaggtgtag ccgtcaggtg tcttcaagac gccgggggaa
     4081 gcagatggca ctggtccggc aggccccgga ccccccggcg caccgggcgg gggagccgcg
     4141 tcgccggccg gtacaggcgg ggccggctca tccgccagtg ccattggagc tgtcccgacc
     4201 gggaccagca tgcaagctgc agccagggtg gcaaaccgat taaacatgtc gcgttggcgc
     4261 ctctcatcgt ccgaattccc cacgggatct gtcctgaggg atctttaagg gagcgagtgt
     4321 ctaaacggtg aacccagcgc taacgctcgg cgcccgattc ccatctaccg ggcgtctcct
     4381 gtcgtcggcc taacttgtgc gcatggcgag aggacgttga cgacatgtaa tggatcgctt
     4441 tgtggcaccg gctgtttgat agtcattcac taaccggaaa gatgttcggc ccaccatcga
     4501 tcgcggagct cgcttcgatc agcatggccg aaatcgcacg atttcgagct ggggtcggga
     4561 gtcgacgact ggaaggaaac gatgaggtca tcttacgttt atgggcggac ggcggccacc
     4621 acactgatgg tattcggcat cggtctggct atcgttccgg cggcatccgc ggaccccggc
     4681 ggtgccgatc agactcaacc gcccgcgcct gctccggccg cgcttcccga tgctcctgca
     4741 cccggtgccg tccctgaggc cgccgcgatg gccggcgcgc ccatcggtcc caccggtgcg
     4801 ccccaaggag tcgcgcctga caccgtccct atgcagactt ccggcgatcc gacgagggac
     4861 gcctgtgacc tcttcaacaa ggcggtcaat tacgcggcga tcaactacga ggacttcgcc
     4921 gactacagcg ctggcagcgg caattacgtg aattaccaag accagactgt cgacaacgcg
     4981 aacgccgccg gtcgaacagc cctgaggcaa gcggccgcgg cagcactcaa tgcgtccggg
     5041 attccgggcg ccgcgcctga ggcgacggcg ccgatgcggt cgtggtccct gagcgccgca
     5101 aagctcgtgt tggtgatggg cgtccgcggc ggcggtgaca ccctcaactc cgcggccacc
     5161 gatctcaaca agactgcgaa agaagcccaa atggcctgtg cgactgccca acagcagtct
     5221 tagtcgcggg gacgtgcagt cacgtcacct tgatgcagga gtgcagggct gacggacgag
     5281 gacaacgaat ggctgacgct ttcgtcatcc acacgagtct gctgggaggg ctgttgactt
     5341 gaccgcgaga atccttggcc caacggtccg gccgggatgt gttcgcgccg cgtctgttcc
     5401 aagcgagtag cacacatttc caggccgatc cgatcgattc agcaaccgac tgatcctgaa
     5461 tcgaaaaaca cggcccaccc gcgtagtgcg ggtaggccgt gcctggagtt cgcgtaccga
     5521 tcaggccgga ggaactggtg tgccgttgtt cactgtccag aacggcaact gaataccggt
     5581 gacctgggcg aagggcttgg tgcccgggac cggcggcttc acgatgaccc ccgtgggagt
     5641 gctcgtcacc gggccgacgg tgaaggggcc ggggttgtaa agactcggcg cgatcgagct
     5701 gagaaccgag ccgatgttgg tgctgctact cgacaggttc cgcgcagccg ttgtctgggc
     5761 attgaccgtc gccgtcggcg tcgagccgct agccggcttg ttcgccgagc cgctatccga
     5821 cttgttcgag tggaagatat tgttgaagcc atcgtttggc ttcgcctgcg ccgtgccggt
     5881 tccgacggtc atggccagtg cggctgccgc cgttgccatt cccgccaagc cggcaacctt
     5941 cgccaggttc gcccggccgt cagtctgtgc tgagtgtcgt cccactgttc gtgtccttcg
     6001 ccatttgctg tttgcggagg ttcttccccc atgtccgcgg acaaccgtat taggtatgca
     6061 caagaatggc atgaccgttc tggtgaattg aaggtggcga accggtgaaa agacgtcact
     6121 aacgtggcga aacagttgcg ctggcgggct aattggtaga actcggatcg gtgaccagct
     6181 actccgtcgg gaatgcctac ctccgctcct atcggaatca cagcgtctga actgtctcca
     6241 agtggcgcga tactgtgcta ggcatgaatt ttgctgtgga tgcgatctaa cgtcacagca
     6301 gcgatggcga ctggagtcgg cgagcggtta ccagggtgcg cggccacatt cgctgctgcg
     6361 tcagacgacg tactttccga accagtcctg cacccggcgc cacgcatcgg cggccgcggt
     6421 tgcgttgtag cggtcccccg tgtcgttgaa gaaggcatga ttggcgtccg gctccgtgat
     6481 gagttcgtgc accagccccg ccttctccag cgcagctctg accaccggct cggttttggt
     6541 cacccggtcg tcgagtgcac cgtagaacgc aagcactgca gcgttcttcg agccggcaaa
     6601 gtctgggtta tcgggggctg gcccgtaaaa cggtacggcg gccgccaact cgggcgtgcc
     6661 tgcggccagc acttgccaga ccagtccccc gcccatgcag aagccgaccg ccgcgatttt
     6721 cttgtcgggt acgcgccgct gcagttcacc gacgcccgac ttgaggttcg cgacgaactg
     6781 ctcgttcggg atgttgccga gtgccgcggt cgcctccgcg gggtcgctga atttcgcggt
     6841 gccgccctgt gccgagagca gatcgatggc gagcgccgag tagccgatgc ctgcgaagcg
     6901 gccggctacc gagcgcaccc aatcgttgag gcccttgttc tcgtgtatga caagcacccc
     6961 gccgcgggcg tccggcgcct ccgcccatgc accctgcagc cca
//
LOCUS       DASYLX010000217         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3848780,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000217 DASYLX010000000
VERSION     DASYLX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848780"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGP27_05225"
     CDS             <1..312
                     /locus_tag="VGP27_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-anti-sigma factor"
                     /protein_id="HEV7850754.1"
                     /translation="NLDVITAYARRFVLTEKPFVLDLTGVNSFTPECISLLYAIDENC
                     HNAGVDWSVVPSQHVHRTLRLFGEGETFPAVGSVAEALHDIADRIGERRRLLPLLTKT
                     A"
     gene            414..1061
                     /locus_tag="VGP27_05230"
     CDS             414..1061
                     /locus_tag="VGP27_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEV7850755.1"
                     /translation="MLGELVPLALDLALSPHSIIPAVVLVLHTDHPRPTGLAFMTGWL
                     VGLLVLTALFVQVPHVIDGLGGSPPWMPLVRIGIGVLLIALAVGRWMTRKRTTRPPAL
                     LNGLSRITPAGAAAVGFGLVVANPKVLVMNAAAGLIIGTAAAGARVWVAVALYTALAG
                     STVIAPVLAYVVAGGRVDQQLERVRNWMQREHAAVTAVILLVVGVLLAYTGIRAP"
     gene            complement(1058..>1847)
                     /locus_tag="VGP27_05235"
     CDS             complement(1058..>1847)
                     /locus_tag="VGP27_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HEV7850756.1"
                     /translation="SADSGLAGRIKHVMIPGMIAEDDDRPVEMKPKLDVLDYWSLIQP
                     ELPGLRGLCTQVTAFMDEAALGERLAILGGAGFEGIVFVGVPRTLNDGEGSGVAPTDA
                     LSIYDRVVDNRGVILIPTREGEHSRFDFKCNQGANFGMTQLLYSDAIVGFLTDFAKNT
                     DHRPEILLSFGFVPKVETRVGLIDWLIQDPGNAAVADEQEFVRSLASRDPADKRQMLV
                     DLYKRVIDGVADLGFPLSIHFEATYGVSGAAFETFAEMLAYWSP"
BASE COUNT          294 a          605 c          593 g          355 t
ORIGIN      
        1 aacctcgatg tcatcactgc ctatgcacgg cgcttcgtct tgacggagaa gcccttcgtt
       61 ttggatctga ccggcgtgaa ttcgtttacg ccggaatgta tttcactgct ctacgccatt
      121 gacgaaaact gccacaacgc cggcgttgat tggtcggtcg ttcccagcca acacgttcac
      181 cggacactgc ggttgttcgg cgaaggtgaa acgttcccgg ccgtcggctc cgtcgctgag
      241 gcgcttcacg acatcgccga tcgcatcggc gaacgtcgtc gcctgctacc tctactcacc
      301 aagaccgcct aacagaaagg gcgatcacgt gctcatcgat ctccgctggt tgacctacct
      361 gctcggcagg cacaccccca catccacgcc cgcgcgctag tccgcccggc ttcttgctcg
      421 gcgagctggt accgctcgca ctagatctcg cactgtcacc gcattcgata attccggcgg
      481 tcgtgctggt tctgcacacc gaccatccaa ggcccacagg cctggccttc atgacgggct
      541 ggcttgtggg ccttctcgtg ttgacggccc tcttcgttca ggtgcctcat gtcatcgacg
      601 ggctcggggg gtctccaccg tggatgccct tggtgcggat cgggatcggg gtcctgctga
      661 tcgccctcgc cgtcgggcgg tggatgacgc gcaagcgcac gacccgcccg cccgccttgc
      721 tcaatggcct gagcagaatc acgcccgccg gcgcggcggc ggtcggcttc gggctggtgg
      781 tggccaaccc caaggtgctc gtcatgaatg ccgccgccgg cttgatcatc ggcacggccg
      841 cagcgggcgc gcgcgtgtgg gtcgccgtcg ctctctacac cgccctggcc ggctcgacgg
      901 tgatcgcccc ggtactcgcc tacgtggtgg ccggcggacg tgtcgatcaa cagctcgaac
      961 gggtgcgcaa ctggatgcag cgggagcacg ccgccgtgac ggcggtgatt ctgctcgtgg
     1021 tcggtgtgtt gttggcctac accgggattc gcgcgcccta gggagaccag taggcgagca
     1081 tctccgcgaa cgtttcgaag gccgcgccgg agacgccgta cgtcgcctcg aagtggatgc
     1141 tcagcgggaa gccgagatcg gcgacaccgt cgatcacccg cttgtagaga tcgaccagca
     1201 tttggcgctt gtcggcggga tcgcggctcg ccagtgaccg gacgaactcc tgctcgtcgg
     1261 cgaccgcagc gttgccggga tcctggatga gccagtcgat caggccgacc ctggtttcga
     1321 ccttcggtac gaagccgaat gacagcagga tttcaggccg atggtcggtg ttcttggcga
     1381 aatcggtgag gaaaccgacg atcgcgtcgg agtacagcag ctgcgtcatg ccgaagttgg
     1441 cgccttggtt gcacttgaag tcgaatcgac tgtgctcgcc ttcgcgggtc gggatgagga
     1501 tcacgccgcg gttgtcgacg acccggtcat agatcgacag cgcatccgtt ggggcgacac
     1561 cggatccttc gccgtcgttc agggtgcggg ggacgccgac gaacacgatg ccctcgaacc
     1621 cggcgcctcc caggatggcc agtcgctcgc caagggcggc ctcgtccatg aaggcggtca
     1681 cctgggtgca cagcccgcgc aaccctggca gctccggctg gatgagcgac cagtagtcca
     1741 ggacgtcgag cttcggcttc atttcgacgg gccggtcgtc gtcctcggcg atcatcccgg
     1801 gaatcatcac gtgcttgatg cggccggcca gccctgagtc cgcggag
//
LOCUS       DASYLX010000218         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2641757,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000218 DASYLX010000000
VERSION     DASYLX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641757"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGP27_05240"
     CDS             <1..673
                     /locus_tag="VGP27_05240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7850757.1"
                     /translation="GMTLLDVGCGWGGALRRAVDVYDVNVIGITLSRNQYEYSKRLMA
                     TLPPSRNAEVRLQGWEEFDEPVDRIVTIGAFEAFKAERYPLFFKRAFDILPADGRMLL
                     HTILAHPQTYFRDNGIKLTINDLKFARFIGTEIFPGGQLPAAEDIEALAAGSGFTLER
                     TQLLRPHYAKTLDMWAESLEANRLQAIEITSDEVYDRYMRYLTGCADFFRRGVTNVGQ
                     FTLVK"
     gene            complement(666..1522)
                     /locus_tag="VGP27_05245"
                     /pseudo
     CDS             complement(666..1522)
                     /locus_tag="VGP27_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
     gene            complement(1622..2437)
                     /locus_tag="VGP27_05250"
     CDS             complement(1622..2437)
                     /locus_tag="VGP27_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEV7850758.1"
                     /translation="MVFHEGDKVADIDTSATPGFKGKKSDAAKVQPKRNERFAELQEM
                     LYANSKGAGDNKSLLLVMQGMDTAGKGGIVKHVVGAANPQGVRYHAFGVPTEEERKHN
                     YLWRITRALPPAGHIGVFDRSHYEDVLVVRVHNLVPPEVWGKRYEEINRFEQNLVDEG
                     TTLIKVAMFISLEEQKKRLCKRLDRPDKYWKYNTKDVDERMLWPQYVEAYQAMLEKTS
                     TDYAPWHVVPCDRKWYGRLAILELLIEALEGLGLSWPPPDFDVEAEKRRLAEA"
BASE COUNT          411 a          807 c          736 g          484 t
ORIGIN      
        1 cggcatgacg ctgctcgacg ttgggtgtgg ctggggcggg gcgctgcgcc gcgcggtcga
       61 tgtttacgac gtcaacgtca tcggaatcac attgagccgc aaccagtatg aatacagcaa
      121 gaggctgatg gcgactctgc cgcctagccg caacgccgag gtgaggctgc agggctggga
      181 ggagttcgac gagcccgtcg acaggatcgt gaccatcggc gcgttcgagg cgttcaaggc
      241 cgagcgctac ccgctgttct tcaagcgggc cttcgacatc ctgcccgccg acggcagaat
      301 gcttctgcac acgatcctcg ctcatccgca gacgtacttc cgcgacaacg ggatcaagct
      361 gacgatcaac gatctgaagt tcgcgcgctt catcggaacc gagatcttcc ccggcggaca
      421 gctgcccgcg gcagaggaca tcgaagcgct cgccgccggg tccgggttca ccctcgagcg
      481 aacgcaactc ttgcggccgc actatgccaa gacgctcgac atgtgggcgg agagcctgga
      541 ggccaaccgg ctgcaggcaa ttgagatcac ttccgacgag gtctacgacc gctatatgcg
      601 atacctgacc ggctgcgcag acttcttccg gcgcggcgtc accaacgtcg gtcagttcac
      661 gctcgtcaag tagcgaggcc cgcgcggtca agtgccgtcc ggactcgacg cacgatctgc
      721 gcgcgttcat atcgtaactg tcttgcgctg accctcacga tgttccatcc cttgtcggcc
      781 aggaactcga gccgttcgat gtcgtcggcg taatcgtctg ggttggtgaa gtgttgttcg
      841 ccgtcgtact cgacgccgac ccggcattcg ggatagccca tgtcgattcg gcgaaccacc
      901 ttgccgtagt cgttcgtcac cgggatctgc gtcctcgggt gcggaagccc agactcgatg
      961 agcagcagtc gcaatcgcgt ctcctgtggc gactccgcac ccccgtctac gagttcgagc
     1021 gtcgcccgca gttgccgcat gcctcgcgcg cccggatagc gatctgcaat cgagcgtgtt
     1081 cgcgcgacgg acacttttgt cgcgttcatc aacgcatcga cgcggactat cgcgttctcc
     1141 ccggtgagtc ttcggccgag gtcgtggcag tcctcgccgg cgtcgtgcac gtcattccgc
     1201 gggcgacctg ttgctcgtcg tcgtcgatcg catcggcacg aatctttatc ccggcgggcg
     1261 catggtggcg cgtgcggccc aactccgccg gtgaatcgcc aggcagccaa cgtgttccga
     1321 ggaccgccgc agccgatgag ccgacgagta tcgcctcacc tcgcgaccac agccacgccg
     1381 cgacagcgcg gtcgagcgcc gtcagctcga cgccggtggg cgcgtagatg ttgcgatgga
     1441 ggttgacgta tcgcgttcgc agatcatgcc gcgtgagcct gccggacgcg agggcctcgg
     1501 acccgatcac aattcttcgc acgccggttg ggcgcgcgaa tcggacaatc ggttccacca
     1561 gactgcggaa gatgacgaaa actgggcaaa attcctcaac taccgcagtc tcgcgatgaa
     1621 gctatgcctc ggcgagccgc ctcttctcgg cctcgacgtc gaaatccggt ggcggccacg
     1681 ataacccgag cccttcgagc gcctctatca acagctcgag gatcgccagc ctgccgtacc
     1741 acttgcggtc acacggcacg acgtgccacg gcgcgtagtc tgtcgacgtc ttctccagca
     1801 tggcctggta ggcctccacg tactgcggcc agagcatgcg ttcgtcgacg tctttcgtgt
     1861 tgtacttcca atacttgtcg ggccgatcga gtcgcttgca cagccgcttc ttctgctcct
     1921 ccagcgagat gaacatcgcg accttgatca gcgtggtgcc ttcgtcgacg agattctgtt
     1981 cgaaccggtt gatctcctcg tagcgcttcc cccacacctc gggcggcacc aggttgtgga
     2041 cccgcaccac cagcacgtcc tcgtagtgcg agcggtcgaa aacgccgatg tggcccgccg
     2101 gtggcagtgc acgcgtgatc cgccacaggt agttgtgctt gcgttcctcc tcggtcggca
     2161 ccccaaacgc gtggtatcgc acgccctgtg gattagccgc tccgacaacg tgtttaacga
     2221 taccgccctt gcccgcagtg tccatgccct gcatcaccag cagtagcgac ttgttgtcgc
     2281 cggcaccctt gctgttggcg taaagcattt cctgcagttc ggcgaagcgc tcgttgcgct
     2341 tgggctgcac cttggcggcg tcggacttct ttcccttgaa acccggcgtc gcgctggtgt
     2401 cgatgtcggc gaccttgtcg ccctcgtgga acaccagg
//
LOCUS       DASYLX010000219         6931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1435446,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000219 DASYLX010000000
VERSION     DASYLX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435446"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGP27_05255"
     CDS             <1..214
                     /locus_tag="VGP27_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HEV7850759.1"
                     /translation="EGMSTGQVSLIVLAVGAGSVFFSHVNDAGFWLVKEYFGMSVGQT
                     IKTWSLMETVLSVTGLVLVLLLGLVI"
     gene            complement(246..737)
                     /locus_tag="VGP27_05260"
     CDS             complement(246..737)
                     /locus_tag="VGP27_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7850760.1"
                     /translation="MTTDPAPGQYESDPRRYPPPFPANLEAGGLAPRFFARLIDGLIV
                     GVVSVVLFLVTDTLSNYWVTGLFTGALTFVYFLAFEITQGWTPGKKLLGLRVHGPGGA
                     PKPTAAQSAIRNAWTLFPIIPIIGGLLGFIAIVVIALTINGSPTKQGKHDELAGGTQV
                     VKG"
     gene            complement(779..1633)
                     /locus_tag="VGP27_05265"
     CDS             complement(779..1633)
                     /locus_tag="VGP27_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7850761.1"
                     /translation="MLIRRIARPLLSAAFIGQGVDALRNPKPAADAARPALEGIRHLP
                     DPIGAKVPSDAETFARINAAVQVGGGLLLATGKAPRLASAALAFTVIPGSIGGHMFWN
                     ESDPQRKADERRALLTDLSLLGGLIIAAVDTEGKPSLGWRGRRAARKMSEAVSSALPV
                     GAATGGALADSAIAERLGHGLHAARERGAELADVARDRAPEIAEAARERAAGLAEIAR
                     ERGPELAEAARERAAELAEVARDRAPEFADAARERAEAARDRAGDLAVTARKESKKQA
                     QKQRRRMS"
     gene            complement(1670..2176)
                     /locus_tag="VGP27_05270"
     CDS             complement(1670..2176)
                     /locus_tag="VGP27_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEV7850762.1"
                     /translation="MDIEHPEANKRWDDDRDETEAQRLDRNWSSLLQELRVSQTGVQL
                     LTGFLLTLPFQQRFTQLDGTMRAVYLVTVACSLGATVLLVAPVGMHRVLFRRHRLDSL
                     VSASHACAFAGLVLLGVALVGVAVVIFNTVVGRTGAWIAGGATLAAFVTFWFLVPLPL
                     RRRSHRSY"
     gene            complement(2226..2783)
                     /locus_tag="VGP27_05275"
     CDS             complement(2226..2783)
                     /locus_tag="VGP27_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7850763.1"
                     /translation="MRKLVITQNMTLDGSIEMLDEWFDPQIQDDELLAESHRQDADSD
                     ALLLGRRTFEDFRGYWPSRSDDKTGITDYLNGVAKYVVSTTMTDPLWMNSTIVARDPA
                     QRIRALKSKPGKDIVVTGSISLAHSLIAAGLVDEYRLFVYPAVQGRGRRLFPEGAPRS
                     TSTLTLAGPPKSFASGIVLLRYASG"
     gene            2843..4243
                     /gene="xylB"
                     /locus_tag="VGP27_05280"
     CDS             2843..4243
                     /gene="xylB"
                     /locus_tag="VGP27_05280"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEV7850764.1"
                     /translation="MTLVAGVDSSTQSCKVLVCDADSGEIVRSGTAPHPDGTEVDPQL
                     WWAALQAAAEKAGGLEDVQAVSVAAQQHGMVCLDGAGQVVRDALLWNDTRSATAADEL
                     VDELGGAGEWAKRIGVVPVAAITASKLRWLADHEPEHCAATAAVCLPHDWLTWRLSGS
                     TDIGDLRTDRSDASGTGYFSAESGAYDHDLLEMATRGRRPRLPTVLGPHDAAGHTPTG
                     ATLGPGAGDNAAAALGLGAQPGDCIVSLGTSGVVSAISHTAPHDAEGIVAGFADATGR
                     QLPLVCTLNGAPVLAAVASMLGVDYDELDRLALSAPTGAGGLTLVPYLEGERSPNLPN
                     AAGALQGMTARNLTAANIARAGVEGLLCSLAYCLDKISAHGVDAQRIILVGGGARSEA
                     VRVIAPAIFGKPVLVPTPAEYVALGAARQAAWTLSQQDSPPGWSFGITTMFTAAPTRE
                     ILDQYRRAQPLTLGQN"
     gene            4248..4940
                     /locus_tag="VGP27_05285"
     CDS             4248..4940
                     /locus_tag="VGP27_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7850765.1"
                     /translation="MNLAWERLADGIHRCRLPFLDVTVGLVCGRTGTLLIDTGTTLLE
                     ANAVKADVAELAGSQVSHMVLTHNHFDHILGSSAFAGATVYCAPEVAATIASRTEELG
                     ADAVRHGADADEVTSAIAALQVPRHRAGEAVLDLGDRSVTITHPGPGHTNHDLIVVVA
                     DAQPAVVFCGDLVEESGDPVIGPDSDKRAWPATLGRVLVAGGPDAAYVPGHGAVVDAE
                     FVQRQGWILRST"
     gene            5050..5280
                     /locus_tag="VGP27_05290"
     CDS             5050..5280
                     /locus_tag="VGP27_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6400 family protein"
                     /protein_id="HEV7850766.1"
                     /translation="MADLDFAYDLTLDESRRRTAVLEAIGDDWDPIAVLAEENKAYDM
                     LYSDLDDEQQRIYDELVGAGVLPDRDRDRVTD"
     gene            complement(5513..6396)
                     /locus_tag="VGP27_05295"
                     /pseudo
     CDS             complement(5513..6396)
                     /locus_tag="VGP27_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878792.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
     gene            complement(6409..>6931)
                     /locus_tag="VGP27_05300"
     CDS             complement(6409..>6931)
                     /locus_tag="VGP27_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7850767.1"
                     /translation="AFAMLTNDDLDLALTYDYNLAPSSPGLVLEAVALWSVKWGLAVP
                     AGGQADIGAYADHTWIVNSRNTADEDAVRTLGSLAGFKPRIAHEIDSLDLVEDLIVSG
                     HGVGLLPINRPTHPGVTVLSLSDPAVVMTAYAVTRRGRATWPPLRVVLDRLSPPFGEP
                     LPWPDWPRPEAAP"
BASE COUNT         1142 a         2281 c         2360 g         1148 t
ORIGIN      
        1 cgaggggatg agcaccggac aggtgtcgct gatcgtgctc gccgtcggag cgggctcggt
       61 cttcttctcc cacgtcaacg acgccggctt ctggctggtg aaggagtact tcgggatgag
      121 tgtgggtcag accatcaaga cgtggtcgtt gatggagacc gtgctctcgg tcaccgggct
      181 ggtgctggtc ctgctgctcg gattggtgat ctgagcgccg ggacgcaagg aggaagtaga
      241 cccggtcagc ccttgacgac ctgggtgccg ccggccaact cgtcgtgctt gccctgcttg
      301 gtcgggctgc cgttgatggt caacgcgatc acgacgatgg cgatgaaccc gagcagcccg
      361 ccgatgatgg gaatgatcgg aaacagcgtc cacgcgttgc ggattgcgga ctgcgcagca
      421 gtcggcttcg gtgcaccgcc ggggccgtgc acacgtagac cgaggagctt cttgccgggc
      481 gtccagccct gggtgatctc gaatgcaagg aagtagacga acgtcagcgc cccggtgaac
      541 aaccccgtca cccagtagtt ggacagcgtg tcggtgacga ggaacagcac aacgctgacg
      601 accccaacga tgagcccgtc aatgaggcgg gcgaagaaac gaggggctaa cccgcccgct
      661 tcgaggttcg cgggaaacgg tggcggatat ctcctcggat cgctttcgta ctgtcctggc
      721 gccggatcgg ttgtcataag cggtcaatct accgaccggc tcgcacgcag ggtgcgactc
      781 aactcatgcg gcggcgctgt ttctgggcct gcttcttgga ctctttcctt gcggtgacgg
      841 caagatcgcc cgcccggtca cgcgcagctt cggcgcgttc gcgcgccgcg tcggcgaact
      901 cgggtgcgcg atcgcgtgcc acctcggcca gttccgcggc gcgttcgcgg gcggcctcgg
      961 ccagctccgg tccgcgttcg cgcgcgatct cggcaagtcc ggcggcgcgc tcacgcgctg
     1021 cctcggcgat ttccggtgcg cggtcacgag cgacgtcggc cagttcggca ccgcgttccc
     1081 gggctgcgtg caggccgtgg cccaaccttt cggcgatcgc gctgtcggcc agcgccccac
     1141 cggtggcggc gccgaccgga agtgcggagc tgacggcctc ggacatcttg cgcgccgcgc
     1201 ggcggccacg ccatccgagc gacggcttgc cctcggtgtc caccgcggcg atgatcaacc
     1261 cgccgagcag gctcagatcg gtgagcaagg cgcggcgctc gtcggccttg cgctggggat
     1321 cggactcgtt ccagaacata tggccgccga tgctgcccgg aataacggtg aaggccaaag
     1381 ccgcagaggc caaccggggc gccttgccgg tggccaacag caaccctccg ccgacttgca
     1441 ccgcggcgtt gatccgcgcg aaggtctcgg catccgacgg caccttcgcg ccgatcggat
     1501 cggggagatg gcggatgccc tcgagggcag gtcgcgccgc gtcggcggcg ggtttgggat
     1561 tgcgaagggc atccacaccc tgcccgatga aagccgctga cagcaggggg cgcgcgattc
     1621 gtcggatcaa catggtcggg gggttccccc ggtcagcgcg gcacaaacgt caataagacc
     1681 tgtggcttcg gcgacgaagc ggtagcggaa ccaggaacca gaacgtaacg aacgcggcga
     1741 gcgtggcgcc gcccgcaatc caggcaccgg tccgtcctac aacggtgttg aaaatgacca
     1801 cggcgacgcc gacgagcgcc acaccgagca ggaccaggcc cgcgaaggcg caggcgtggg
     1861 aagccgacac gagtgaatcc aaacggtgcc tgcgaaacaa caccctgtgc atgccgaccg
     1921 gcgcaaccag cagcacggtc gcgccgagcg aacacgcgac cgtgacgagg tagaccgcgc
     1981 gcatcgtgcc gtcgagttgg gtgaagcgtt gctgaaacgg caaggtgagc aggaaaccgg
     2041 tgagcagctg aacaccggtc tgactcaccc tcagctcttg cagcaggctt gaccaattac
     2101 ggtccagccg ttgtgcttcg gtttcgtcgc gatcgtcatc ccatcgcttg ttcgcttcgg
     2161 gatgctcgat gtccacgaca cacagtgtgg cagcacgacc gcaccctatg agcggttcgg
     2221 ccgtttcacc cgctggcgta ccgcagcaaa acgatgcccg acgcgaatga cttcggcggt
     2281 ccggccaacg tcagcgtcga cgtcgatcta ggcgcgcctt cgggaaacag cctgcggccg
     2341 cgaccctgga cggcggggta gacgaagagc cggtactcgt ccaccaggcc ggccgcgatc
     2401 aagctgtggg cgaggctgat gctgcccgta accacgatgt cttttccggg cttcgacttc
     2461 aaggcgcgga tccgctgcgc ggggtcgcgc gcgacgatcg tcgagttcat ccacagcggg
     2521 tcggtcatcg tcgtcgagac cacgtatttg gccaccccgt tcaggtagtc ggtgatcccg
     2581 gtcttatcgt cgctgcgact cggccagtaa ccgcggaaat cctcgaaggt acggcgcccg
     2641 agcagcagcg cgtcggagtc ggcgtcctgg cggtggctct cggccagtaa ctcgtcgtcc
     2701 tgtatctgtg ggtcgaacca ctcgtcgagc atctcgatcg acccgtccag cgtcatgttc
     2761 tgggtgatca cgagctttcg cataccggta ctgacttcgc ggcgttggcg aactcatcgc
     2821 ccgtttcggg tagcttcgca acgtgaccct cgttgccggc gtcgactcct ctacgcagtc
     2881 gtgcaaggtg ctggtgtgcg atgcggacag cggtgagatc gtgcggtccg gaaccgctcc
     2941 gcatcccgac gggactgagg tggatcctca actctggtgg gcggccctgc aggccgccgc
     3001 cgagaaggcg ggtgggctcg aggacgtgca agccgtctcg gtggccgccc agcagcacgg
     3061 catggtctgt ctggacggcg cgggccaagt cgtccgggat gcgctgctgt ggaatgacac
     3121 tcgctcggca acggcagccg atgaactcgt cgacgaactt ggcggagcgg gtgaatgggc
     3181 taaacgcatc ggcgtggtgc cggtagccgc gatcacggcg agcaagctgc gctggctcgc
     3241 cgaccacgaa cccgaacact gtgccgccac tgcggcggtg tgcttgccgc atgactggct
     3301 cacatggcgg ctgagcggtt cgaccgacat cggcgatctg cgcaccgacc gcagcgacgc
     3361 cagcggaacc gggtacttct ccgcagaatc cggcgcctac gaccacgacc tgctcgaaat
     3421 ggcgacgcga ggcaggcgcc ccaggctgcc cacggtgctc ggcccgcacg acgccgccgg
     3481 ccacacgccg accggcgcca cgctcgggcc gggtgcgggc gacaacgcgg cggcggcatt
     3541 gggtctgggc gcccaacccg gcgactgcat cgtctccctc ggcacatccg gtgtggtgag
     3601 cgcgatcagt cacaccgcac cgcatgacgc cgaggggatc gtcgccggat tcgccgatgc
     3661 gacgggacgc caactgccgt tggtctgcac cctcaacggg gcgccggtgc tggctgcggt
     3721 cgcgtcgatg ctcggcgtgg actacgacga gctggaccgg ttggcgttgt cggcgccgac
     3781 gggtgcgggc gggctgacgc tggtgcccta cctggagggt gaacggtcac cgaacctgcc
     3841 gaacgccgcc ggggctctgc aggggatgac ggcgcgcaac ctgaccgctg ccaacatcgc
     3901 gcgcgccggt gttgagggac tgctttgctc gctggcgtac tgcctcgaca agatctcggc
     3961 ccatggcgtg gacgcccagc ggatcattct ggtgggtgga ggggcgcgtt ccgaagcggt
     4021 tcgcgtcatc gcgcccgcga tcttcggcaa gccggtactg gtgcccacac ccgcggaata
     4081 cgtggcgctg ggcgcggccc gacaggcggc ttggacactg tcgcaacagg attcaccgcc
     4141 ggggtggtca ttcgggatca ccacgatgtt caccgccgcg ccgacgcgcg agatactcga
     4201 ccagtaccgg cgggcccagc cgctgacgct gggtcagaac tgaccgggtg aacctggcct
     4261 gggagcggct ggccgatggc atacatcgtt gccgtctgcc gtttctcgac gtcaccgtcg
     4321 gcctggtgtg cggcaggacg ggcacgctgc tgatcgacac cggaacgaca ctgctcgaag
     4381 cgaacgccgt caaggccgac gtcgcggaac tcgccggcag ccaggtcagc catatggtgt
     4441 tgacgcacaa ccacttcgat cacattctcg gatcgtcggc cttcgccggc gcaacggtgt
     4501 actgcgcgcc cgaggtggcg gcgacaatcg ccagtcgaac agaagagctg ggggcagacg
     4561 cggtgcgcca cggcgccgat gccgacgagg tgaccagcgc catcgccgcg ctccaggtgc
     4621 cccggcacag ggccggtgag gcggtgctgg accttggcga cagatcggtg acgatcaccc
     4681 atcccggacc tgggcacacc aatcacgacc tgatcgtcgt cgtggcggat gcgcagcctg
     4741 cggtcgtctt ctgcggcgac ctcgtcgagg agtccggcga tccggtcatc ggtccggact
     4801 ccgacaaaag ggcctggccc gcgacgctcg gccgggtgct cgttgcgggc gggccggatg
     4861 cggcatatgt gccggggcac ggcgcggtcg tcgacgcgga gttcgtccag cgccagggat
     4921 ggatcctgcg cagcacgtaa gtgtgactaa tttcgcactg gcgacgtgac ctaattcatt
     4981 gcctaacccc gcgcgcgtag gcaactcttc cagtctcgaa tcgcatgact gaagggtggc
     5041 aggtgcccca tggccgacct cgacttcgcc tatgacctga cgctggacga gtcccggcgg
     5101 cgcaccgccg tgctcgaagc gatcggtgac gattgggacc cgatcgccgt tctcgccgag
     5161 gagaacaagg cgtacgacat gctctactcc gacctcgacg acgagcagca acgcatctac
     5221 gacgaactgg tcggcgcggg agtcctccct gatcgggatc gtgaccgtgt taccgattga
     5281 tccgaccgca gaccgagccc ggcgcgcctg gctcgagtgc ccccgctgcg agcacggtcg
     5341 cgcgtgcgga gattgtcaga gcgcccgcaa ctgcgccacc cactggcagt tcctgctggc
     5401 caatcagggc actttggtgc acctacagtg ccccacctgc gcccatgtct ggtcgaccga
     5461 cacccgcatg cggggctcaa ggcggtccaa agccgcctga ttcgcggcct ctttagttcc
     5521 gtagctgcgg cagtacgtcg cgttcccagc tggtgaagaa cccgtccatg tcgagtccga
     5581 cctgctgcac gtagacctcg tcgacgtcgg cgtcgagata cgtccgcacc tatgcgatgt
     5641 gtttgtcagc gtcgggtccg cagacgacgg attccgcgac ggtttcgcgc ggcaccaggg
     5701 tcatcgcatc cgcgaaatcc ctcggccggg gcagcgtctg cgccaactgt cccggcaggc
     5761 cctcgttggc ccacagccgg tgtgcgacgt caagggcgac gtccgggtcg cggtgccagc
     5821 tgaccttggt tccggcctgg acgggtttgt cgccaccgcc tgctccccgg aatgtcttca
     5881 cgagttcggc gtccggcatc gccatgcagt agccgtcgcc gatccggcct gcgagttccg
     5941 cggcctgagg tccgaagccg gaaacgtaga tcggcacggg ttggtcgggc agtgtgtaga
     6001 tgcgtgcttc ctgtacttcg taataccgcc cgtgatggct gatctcgtca ccgcggtgca
     6061 gcagccggat cacgtcgacg gcctcctcga gcatctgctg gcgaacgccc actgacggcc
     6121 acgcatggcc cagcacgtgt tcgttgagcg cttcgccgct gccgacgccg agaacgaact
     6181 tgccgtcgag ttgcaccccg gcggtcgccg acgcctgggc gatgatggcg gggtggatgc
     6241 ggatggtggg acaggtcacc gccgtcgtga ccggtaacga cgtcgcctcc gacaccgcac
     6301 cgatgacacc ccagacgaac gggctctgtc cctgttcgtc gttccaaggg tggaagtgat
     6361 ccgaaaccca agtgcttcaa agcctgcggc ttccgcttcg ggcttgagct acggagccgc
     6421 ctcggggcgg ggccagtccg gccacggcaa cggctcgccg aacggcgggc tcagccggtc
     6481 gagcaccaca cgcagcggcg gccatgtcgc ccggcctcgc cgagtcacgg cgtaggcggt
     6541 catcaccacg gcgggatccg agagcgaaag cacggtgacg ccggggtgtg tcggcctgtt
     6601 aatcggtagc agcccgacac cgtgtccgga cacgatcaaa tcctccacca gatccaggct
     6661 gtcgatttcg tgggcgatgc gcggcttgaa tcccgcgagc gagcccagcg tccgcacggc
     6721 gtcctcgtcg gcggtgttgc gcgagttgac gatccaggtg tggtccgcgt acgcgccgat
     6781 gtctgcctgc ccgccggcgg gcacggccag tccccatttg accgaccaca gcgcaacggc
     6841 ttccagcacg aggccggggg aggagggcgc gaggttgtag tcgtaagtca gcgccagatc
     6901 gagatcgtcg ttggtgagca tcgcgaacgc c
//
LOCUS       DASYLX010000220         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1984502,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000220 DASYLX010000000
VERSION     DASYLX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984502"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGP27_05305"
     CDS             <1..309
                     /locus_tag="VGP27_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEV7850768.1"
                     /translation="ITSLDNGTASLADLLGRARPPLAGTVDQLNRLAPLVDGAHKDYL
                     DATLQQLPELYRKLARVGSYGSFFPYYICGITFRASDLEGRTVVFPWIKQETGRCAEN
                     "
     gene            318..1421
                     /locus_tag="VGP27_05310"
     CDS             318..1421
                     /locus_tag="VGP27_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7850769.1"
                     /translation="MDRYRGSQLIQAGIIGVVLMILIIIAGLQPDRLLEYATGIKYQA
                     VFTEAGGLVEGNDVTVSGTKVGAVSSIELDNGDALVTFTIAGKAALGSETTAHIRTGT
                     LLGERVLALESSGSGTLDHQKPIPVTRTSSPYSLTDAVSELTANTAATNTDTLNQSLD
                     TLATTLDQISPQLGPTFDGLSRLSRSLNDRNESLAELLKTAGDVTGILSGRSQQVNTL
                     ILNANDLLAVLNERREAIVSLLANTSAVSRELTGVVADNEQKLAPTLERLNRVNAMLI
                     KNRDNISKAIPGLSKYELTQGEIVSNGAYYNAFIPNISPAQILQPFLDYVFGFRRGVG
                     AGQPPDNAGPRAELPLPVNGIPQPGDLPDDGNP"
     gene            1405..2892
                     /locus_tag="VGP27_05315"
     CDS             1405..2892
                     /locus_tag="VGP27_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7850770.1"
                     /translation="MMATRKRLAVVTAVILAIALVAGVVVLVRQVFFGPNTITAYFTT
                     ATAIYPGDEVRVSGVKVGKIDSIEPDGTQTKMTLKVDHGVPVPADAKAVIVAQNLVAA
                     RYVQLTPAYRKGGGPTMRDGAVIPVDRTAVPVEWDEVKDQLMRLATDLGPKTGVSGTS
                     VSRLIDSAANALDGNGDKLRQTLAQLSGAARIFAEGSGNVVDIIKNLQTFVTALRASK
                     EQIVMFENRLASLTSVVNDSRSDLDAALSDLSVALGEVQRFVAGSRNQVSEQVSSLAE
                     VTQILVDNRSAVENILHITPNAVANYQNIYYPPSGGVSGAFSLSNFANPVYFFCGLIG
                     AIENTTAPETAKLCSQYLGPALRLLNFNNLPLPINLYLRPAPKPENIIYTDPKLAPGG
                     AGPGDPPEAPPTVSAYTGAGDIPPPPGWGAPPGPPGLYSPGGEVPATPSPALFPGAPI
                     PGPPTVLPGPPSTVDSMLLPSTPAPAAPPVSPNAPLLPAEGTPPS"
     gene            2889..>3430
                     /locus_tag="VGP27_05320"
     CDS             2889..>3430
                     /locus_tag="VGP27_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7850771.1"
                     /translation="MIRAAHATSRACGSRVRNVVIVGSCLALTASGCAFEGLNSLPLP
                     GAVGRGPDAVKYHVEVPNVSTMESNSPVMINDVVVGSVGTMKVDQWHADVEISVKPDV
                     VVPANAVATVGQTSLLGSMHLALNPPLGQAPSGRLQPGATIGLNRSSAYPTTEQTLSS
                     LSVIANGGGLGQLGDIIHNT"
BASE COUNT          655 a         1145 c         1042 g          588 t
ORIGIN      
        1 atcacgtctc tagacaacgg caccgcctcg ctggccgact tgctcggccg ggcacggccg
       61 ccgctggccg gcacagtcga ccagttgaac cggctggctc ctctggtgga cggcgcgcac
      121 aaggattatc tcgacgccac cctgcagcag ctgcccgagc tctaccgcaa acttgcgcgg
      181 gtcggttcct acgggtcgtt cttcccctac tacatctgcg ggatcacctt ccgagccagc
      241 gatctcgagg gccgcaccgt cgtcttcccc tggatcaaac aagagacagg gaggtgcgcg
      301 gaaaactaat ggcactaatg gatagatatc gcggatctca actcattcag gcgggcatca
      361 ttggcgtcgt cctgatgatc ctcataatca tcgcgggcct tcagccggac cggttgctgg
      421 aatacgccac cggcatcaag taccaggccg tgttcaccga ggcgggcggt ctcgtcgaag
      481 gcaacgacgt gaccgtgtcg ggtaccaagg tcggcgcagt gtcgtcgatc gagctcgaca
      541 acggtgatgc gctggtcaca ttcacgatcg cgggcaaggc cgccctcggg tcggaaacca
      601 ctgcgcacat ccgcaccgga accctgctcg gcgagcgtgt gttggccctg gagtcttcgg
      661 gaagcggcac gctggaccac cagaagccca ttccggtgac gcgcacgtcg tcgccgtact
      721 ccttgactga cgctgtcagt gaactcaccg cgaataccgc tgcaaccaat acggacacgc
      781 tgaaccagtc gctggacacg ctggcgacga cgctcgacca gatctcgcca cagttgggcc
      841 cgaccttcga cggcctgtcc cgactttcga gatcgctcaa tgatcgcaac gagagtctgg
      901 ccgagcttct gaaaaccgcc ggcgacgtga ccggcatcct gtccggacgt agccagcagg
      961 tcaacacgct gatcctcaac gccaacgatc tactcgcggt gctcaacgag cgtcgagagg
     1021 ccatcgtcag cctgctcgcc aacacctcgg cggtgtcgcg ggagctgacc ggcgtggtcg
     1081 ccgacaacga gcagaaactg gcaccgacgc tggaaaggct gaaccgggtc aacgcgatgc
     1141 tcataaagaa ccgggacaat atttccaagg ccatccccgg cctgtctaaa tacgagctca
     1201 cacagggcga aatcgtgtcg aacggcgcct actacaacgc gttcataccc aacatttccc
     1261 cggctcagat cctgcagccg ttcctggatt acgtcttcgg gttccgtcgg ggtgtgggcg
     1321 caggccagcc gccggacaat gccgggccgc gcgccgaact gccgctcccg gtcaacggca
     1381 tcccgcaacc aggagacctg cccgatgatg gcaacccgta agcgcctcgc ggtggtcact
     1441 gcggtaatcc tggccatcgc actcgtcgcc ggagtcgtgg tgttagtgcg ccaggtcttc
     1501 ttcgggccca ataccatcac cgcgtacttc acgaccgcca cggcgattta ccccggcgac
     1561 gaggtgcgcg tgtcgggtgt caaggtcggc aagatcgact ccatcgaacc cgatggcacg
     1621 caaacgaaga tgaccttgaa ggtcgatcac ggcgtgcccg ttccggctga cgccaaggcg
     1681 gtgatcgtcg cccagaatct ggtggcggcg cgttacgttc aactcacgcc ggcctaccgc
     1741 aagggcggcg ggcccaccat gcgtgacggc gcggtcatac ccgtggaccg aaccgcggtc
     1801 cccgtcgagt gggatgaggt aaaagatcaa ctgatgcgcc tggcaacgga tttgggtcca
     1861 aagaccgggg tgtcggggac ctccgtttcc aggttgatcg acagcgcggc gaacgcgctg
     1921 gacggcaacg gcgacaagct acgccagacg ctggcccaat tatccggcgc tgcaaggatc
     1981 ttcgccgaag gcagcggcaa cgtcgtcgac atcatcaaga atctgcagac cttcgtgacc
     2041 gcgctgcgtg ccagcaaaga gcagatcgtc atgttcgaga atcggctggc cagtttgacg
     2101 agtgtcgtga acgacagcag gtccgatctg gatgcggcgc tgtcggactt gtcggttgcg
     2161 ctcggcgagg tgcagcgctt cgtcgccggc agtcgcaacc aagtatccga gcaggtcagt
     2221 agccttgccg aggtcaccca gatcctggtc gacaaccgct cggctgtcga gaacatcctt
     2281 cacatcacgc cgaacgcggt cgccaactac cagaacatct actacccgcc ctccggcgga
     2341 gtgagcggag cattctcgct gtcgaacttt gcgaatccgg tctatttctt ctgcgggttg
     2401 atcggcgcaa tcgagaacac caccgctccc gaaacggcga aactctgctc gcaatatctc
     2461 gggccggcgc tgcgattgct caatttcaac aacctgccgt tgcccatcaa cttgtacctg
     2521 aggcctgcgc ccaaaccgga aaacatcatc tacaccgacc cgaagctggc gcccggaggc
     2581 gccgggccgg gcgacccgcc cgaagcgccg cccacagtgt ccgcctacac cggcgccggc
     2641 gatatcccac cgccgccggg ctggggagcg ccaccgggcc cgccggggtt gtactcgccc
     2701 ggcggagagg tacccgctac accgtcgcct gcgttgttcc ctggcgcacc gattccgggg
     2761 ccccccactg tgctcccggg acctccgtcg accgttgaca gcatgttgct tccgtcgacg
     2821 ccggcgcccg ccgcgccgcc ggtctcgccc aatgcacccc tgctgccagc cgaagggacg
     2881 ccgccatcat gattcgcgcc gcgcacgcga cgagcagggc ttgcgggagt cgtgtgagga
     2941 acgtggtgat cgtgggatcg tgccttgcgt tgacggcgtc cggatgcgcc ttcgaaggcc
     3001 tgaattcctt gccgctaccc ggagctgtcg gacggggacc cgacgccgtc aagtaccacg
     3061 tcgaagttcc gaatgtctcc acgatggagt cgaattcgcc ggtgatgatc aacgacgtcg
     3121 tcgtcggcag cgtgggcacg atgaaggtcg accaatggca tgccgacgtc gagatctcgg
     3181 taaagccgga cgtggtggtg cccgccaacg cggtggccac cgtcgggcag accagcctcc
     3241 tgggttcgat gcacttggcg ctcaacccgc cgctcggcca agcgccaagc ggcagattgc
     3301 agccgggtgc cactatcggc ttgaaccggt cctcggcgta tccgaccacg gagcagacac
     3361 tgtcatcgct gtcggtgatc gccaacggag gcggtctggg gcagctcggt gacatcatcc
     3421 acaacaccaa
//
LOCUS       DASYLX010000221         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3410641,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000221 DASYLX010000000
VERSION     DASYLX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3410641"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VGP27_05325"
     CDS             <1..122
                     /locus_tag="VGP27_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850772.1"
                     /translation="MADSGHNLSVGWSASEYHRRVLSFVGECAAARETELEAG"
     gene            122..940
                     /locus_tag="VGP27_05330"
     CDS             122..940
                     /locus_tag="VGP27_05330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850773.1"
                     /translation="MRVGFIGLGSQGAPMARRIVEAGYDLTLWARRPATLETFADTAA
                     KRAQSPAELAAASDLVCVCVVGDDDVREVFGGDTGVLAGLASGGIVAIHSTVHPDTCR
                     ELAESAAGQGISVIDAPVSGGGPAAEEGQLLVMVGGDDEVVERCRPVFSTYADPIIHL
                     GPLGSGQVTKILNNVLFTANLGGAISTLELGESLGIERHRLCEVLGSGSASSRALGSI
                     SAFGGTLDGLAPIAGALLQKDVRHAATLADTARAARPAVFDGADVALNAMGYPR"
     gene            937..1689
                     /locus_tag="VGP27_05335"
     CDS             937..1689
                     /locus_tag="VGP27_05335"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850774.1"
                     /translation="MTRVGFVGVGRMGAPMVRRLVDAGHDVRALGRTPEKTDAIAQLG
                     AHAVTEPAAVAAGADAVVVCVFTDDQVRQICLEKDLVAAMSPDAVLVIHTTGSPRTAQ
                     MIATRGIDVVDSPVSGGPHDIEAGRVTLFVGGDDEAVTRARPLLSAYGDPILHVGRTG
                     AGQLVKLINNALFAAQIGLVAESVRLGSLLGIAEPALLDALTHGSADSRALGLISRAG
                     STAAFTETVGDFIDKDVAVVRETVADLGGSVR"
     gene            1848..3050
                     /locus_tag="VGP27_05340"
     CDS             1848..3050
                     /locus_tag="VGP27_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850775.1"
                     /translation="MTKPKLVFNPVSQDFFDNPYEIYGRMRDEAPIYYDEEEDFYALT
                     QHADVAAAFKHHEGFSSARGCDLGMVRSGEVPQKSIIFMDPPDHRHMRSLLNKAFTPR
                     AIQAQRETVTELIEHYLGKADPDNFDVVQDFSGPFPVEVITRMAGVPEEFRQQVRHWI
                     DTSLHREPGQIDWSEENQKANIDSGMYYYGLVLERRENPQDDMISRLIAAEIPGEDGQ
                     MRKLDDIEITGFTTLLGGAGAETVTKLLGSAVVEFARHPEQWQLLLDDRTRIPDAVEE
                     LLRYVGPVQYNVRYSVQDVELPSGTVAAHKPVFLMGASANRDPRAFDDAETFDITRDR
                     LQAQNLGLGYGIHSCLGAALARMETAIALDRLLDFMPRFEVDFDGLERVSMQNVAGYH
                     RVPVKVLK"
     gene            3047..3247
                     /locus_tag="VGP27_05345"
     CDS             3047..3247
                     /locus_tag="VGP27_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7850776.1"
                     /translation="MTQKVEVDFGLCESNGICMGIIPEVFDLDEQDYLHIMQDEVTSE
                     NEDQIREAVRQCPRQAISIRDE"
     gene            3259..>3365
                     /locus_tag="VGP27_05350"
     CDS             3259..>3365
                     /locus_tag="VGP27_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7850777.1"
                     /translation="MRFVLVHGGFHAAWCWDHTIAELRRLGHDGVAVDLP"
BASE COUNT          623 a         1074 c         1117 g          551 t
ORIGIN      
        1 agatggcaga cagcggacac aatctcagcg tcggctggag cgcgagcgaa taccaccggc
       61 gggtgctgtc gttcgtcggc gaatgcgcgg cggcacgcga aacggaactg gaggcgggtt
      121 gatgcgcgtt ggcttcatcg gactgggcag ccagggcgcc ccgatggcgc gacggatcgt
      181 cgaagcaggt tacgacctga cgttgtgggc gcgtcggccc gccaccctcg aaaccttcgc
      241 cgacaccgcc gcgaagagag cgcagtcgcc cgccgaactc gccgccgcca gcgatctggt
      301 gtgcgtgtgt gtcgtcggcg acgacgacgt cagagaggtc tttggcggcg acaccggcgt
      361 gctggccggc ctggcgtcgg gcggaatcgt cgccatccac agcaccgtgc atcccgacac
      421 ctgccgagag ttggccgaat ccgctgcggg gcaaggcatt tcggtgatcg atgctccggt
      481 gagcggcggg ggccccgccg cggaggaagg ccagctgctc gtcatggtcg gcggcgacga
      541 cgaggtcgtc gagcgctgcc gaccggtgtt ctcgacctac gccgacccga tcatccatct
      601 gggtccgctg ggcagcggac aggtcaccaa aatcctgaac aatgtgctgt tcaccgcgaa
      661 tctgggcggc gcgatcagca cactcgaact cggcgaatcc cttggcatcg aacgccatcg
      721 gctgtgtgag gtgctgggca gcgggtcggc cagcagcagg gcgctcggca gcatctctgc
      781 cttcggcgga acgctggacg gccttgcgcc gatcgcgggg gcactgctgc agaaggatgt
      841 ccggcacgcc gccaccctcg ccgacacggc gcgggcagcg cgccctgccg tgttcgacgg
      901 ggcggacgtc gcgctgaatg cgatgggata tccgcgatga cccgcgttgg cttcgtcggc
      961 gtggggcgaa tgggtgcacc catggttcga cgactcgtcg acgccggcca cgacgtccgc
     1021 gcgctcggcc ggacacccga aaagaccgat gccatagcgc aactgggtgc tcacgccgtg
     1081 accgagccgg cagcggtcgc cgcgggtgcc gacgccgtcg tcgtctgcgt gttcaccgat
     1141 gatcaggtgc ggcagatctg tctggaaaaa gatctggtcg ccgccatgtc gcccgacgcc
     1201 gtcctcgtca ttcacaccac gggcagccca cgcaccgctc agatgatcgc cacccgcggc
     1261 atcgacgtgg tcgattctcc cgtcagcgga ggccctcacg acatcgaagc gggacgggtg
     1321 acgctgttcg tcggcggcga tgacgaagcg gtgacgcgtg cgcgaccatt gctgagtgcc
     1381 tacggtgacc cgatcctgca cgtcggccgc acgggtgcgg gtcagctggt gaagctgatc
     1441 aacaacgcgc tgttcgccgc gcagatcggg ctggttgccg agagcgtgcg gctgggcagc
     1501 ctgctcggca tcgccgaacc ggcactgctg gacgcgctga cgcacggcag cgccgacagc
     1561 cgtgcactgg gcttgatcag cagagccgga tcgaccgcgg cctttaccga gaccgtcggc
     1621 gacttcatcg acaaggacgt cgcggtggtc cgcgagacgg tcgccgatct gggtggctcg
     1681 gtgcgctgaa ccgcgtggtc gacgtcgtgc tcaacaaatg agacacattt cccgaatgtc
     1741 tcattctctt ttgcggaaac gccgttaccg ttagggttac agtcgttaca agtcgactgc
     1801 gtctcgtaac gctccccggc cgatcagccc gccgcgaaag aggagaaatg accaagccca
     1861 aattggtgtt caacccggtt tcgcaggact ttttcgacaa tccctacgag atctacgggc
     1921 ggatgcgcga cgaggcaccg atttactacg acgaggaaga ggacttctac gcgctgacgc
     1981 aacatgccga cgtggccgcc gcgttcaagc accacgaggg gttctcctcc gcgcgtggct
     2041 gcgacctggg aatggtgcgc tccggcgagg taccacagaa gtcgatcatc ttcatggacc
     2101 cacccgacca caggcacatg cgcagcctgc tgaacaaggc gttcactcca cgggccattc
     2161 aggctcagcg cgagaccgtc accgaactga tcgagcacta tctcggcaag gccgacccgg
     2221 acaacttcga cgtggtgcag gacttctccg gtcccttccc ggtcgaggtc atcacccgca
     2281 tggccggcgt gcccgaagaa ttccgtcagc aggttcggca ctggatcgac accagtttgc
     2341 accgcgagcc gggtcagatc gactggagtg aagagaacca gaaggccaac atcgactcgg
     2401 ggatgtatta ctacggcctg gtgctggaac ggcgcgaaaa tccgcaggac gatatgatca
     2461 gccgcctcat cgccgccgag attcccggcg aggacggcca gatgcgcaag ctcgacgaca
     2521 tcgagatcac cggattcacg acgctgttgg gtggcgcggg cgccgaaacc gtcaccaaac
     2581 tgctcggcag cgcggtcgtc gagttcgccc ggcacccaga gcaatggcag ttgctattgg
     2641 acgatcgcac caggattccc gacgccgtcg aggagctgct tcggtacgtc gggccggtgc
     2701 agtacaacgt gcgctacagc gtgcaggacg tggaattgcc aagcgggaca gtcgcggccc
     2761 acaagccggt gttcctcatg ggggcgtccg ccaatcgcga tccccgcgcc tttgacgacg
     2821 ccgagacgtt cgacatcacg cgagaccggc tgcaagcaca gaacctcgga ctgggctacg
     2881 gaattcacag ctgcctcggc gcggcgctgg cgcgcatgga aaccgcaatc gcgctggacc
     2941 gccttctcga tttcatgccg cgattcgaag tggacttcga cggcttggag cgggtcagca
     3001 tgcagaacgt ggcgggctac caccgcgtac cggtgaaagt tctgaaatga cccagaaggt
     3061 agaagtcgac ttcggcttgt gcgagagcaa cggcatctgc atgggcatca tcccggaggt
     3121 cttcgacctc gacgagcagg actatctgca catcatgcag gacgaggtga cctcggagaa
     3181 cgaggaccag atccgcgaag cggtccggca gtgcccgcgg caggccattt ccatccgcga
     3241 cgagtgaagc cgaactggat gcgattcgtg ttggtgcacg gcgggtttca cgccgcctgg
     3301 tgctgggacc acacgatcgc cgaactgcgg cggctcggcc acgacggtgt ggccgtcgac
     3361 ctgcc
//
LOCUS       DASYLX010000222         5453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_996460,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000222 DASYLX010000000
VERSION     DASYLX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996460"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VGP27_05355"
     CDS             complement(<1..133)
                     /locus_tag="VGP27_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850778.1"
                     /translation="MFGSMFAAASDEELVAAIEDGVRQEAIAGARRLSAIAELTRRRI
                     "
     gene            288..1145
                     /locus_tag="VGP27_05360"
     CDS             288..1145
                     /locus_tag="VGP27_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7850779.1"
                     /translation="MDNISGRTIAITGAARGIGFAAAKALLVRGARVVIGDRDVAFLE
                     SAVADLTKLGPVSGYPLDVTDRASFATFLDKARTDGGGHIDVLINNAGVMPIGPFLDQ
                     SEQSIRSSIEVNLYGVITGCQLALPDMIARRCGHIINIASLSGLIPVPGQVVYVGTKF
                     GVVGLTAALADEVAPHGIDVSVIMPPFTNTELISGTKTGGIKPVEPEDIAAAIIKTLE
                     KPKTHVSVPTPLRFTAQAAQMLGPRGRRWLNKRLGLDTVFMTFDHAKRQSYEQRAQAA
                     QGVVESPER"
     gene            complement(1115..1519)
                     /locus_tag="VGP27_05365"
     CDS             complement(1115..1519)
                     /locus_tag="VGP27_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850780.1"
                     /translation="MSRAAEAKKARRNKRRAKRDVNWIPDSVLDDIVDDVELVEDLES
                     FDARITERGWTFDEEQSDEEFVIWFYPPSGAEVGKGLEPVTTIWMHVAEEAEVVHTIL
                     VGTADDQPMTPDEFFGRIDAIEAYRSGDSTTP"
     gene            complement(1529..2749)
                     /locus_tag="VGP27_05370"
     CDS             complement(1529..2749)
                     /locus_tag="VGP27_05370"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEV7850781.1"
                     /translation="MSNNPDTPSVRTPAKLPEGVSQATIGVRGGLLRSGFEETAEAIY
                     PTSGYVYASAAEAEKAFTGEIDRYVYSRYGNPTVSMFEERLRLIEGAPAAFATATGMA
                     AVFTSLGALLAAGDRLVAARSLFGSCFVVCNEILPRWGIETVFVDGEDLSQWEEALSV
                     PTQAVFFETPSNPMQSLVDIAAVSEMAHGVGAKVVLDNVFATPILQQGLPLGVDVVVY
                     SGTKHIDGQGRVLGGAILGEAAYIDEPVQKLMRHTGPALSPFNAWVLLKGLETLAVRV
                     QYSNDSAVRVAQFLESHPAVGWVRYPFLESHPQYDLAKRQMTGGGTVVTFELKGGSKE
                     RAFEVLDKLRIVDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISVGLE
                     GTEDLINDLDQALG"
     gene            complement(2746..3180)
                     /locus_tag="VGP27_05375"
     CDS             complement(2746..3180)
                     /locus_tag="VGP27_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7850782.1"
                     /translation="MSYAGDITPEEAWKMLNDDPQAVLVDCRTDAEWRFVGVPDLTGL
                     GRDVIYVEWNRTDGNRNDGFVDDLVAAGVEPGVGPDAGGGRPVVFLCRSGNRSIGAAE
                     AATEAGIAPSYNILDGFEGDLDENKHRGATGWKAVGLPWKQS"
     gene            complement(3200..3664)
                     /locus_tag="VGP27_05380"
     CDS             complement(3200..3664)
                     /locus_tag="VGP27_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lumazine-binding protein"
                     /protein_id="HEV7850783.1"
                     /translation="MPSDDEAVPADDEAADRPTIAPFLGALALIVIVVIAIWLFNVLG
                     ADRTPQDQLVGRTAVGQNDALQREDFADFGTYMCAKMRGVEADVIAHQRDSVVKRGAR
                     FVDDVTDIKIDGDRATATVTYHFDKAPDAKAPVPVTFVREDGAWKVCSTGPS"
     gene            complement(3667..4929)
                     /gene="purT"
                     /locus_tag="VGP27_05385"
     CDS             complement(3667..4929)
                     /gene="purT"
                     /locus_tag="VGP27_05385"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEV7850784.1"
                     /translation="MTETTESEVPEDTPDEEPTDNEPGQHHDDRKAVMLLGSGELSRE
                     LTLAFQRLGREVVAVDRYANAPAHGVADRAAVVKMNDAEALSAIIERANPLYVVAESG
                     VIAVDALVAAAERGLEVFPTPRGVRMSQDREGMRRMAADELGLPTAPFWFAGSVDELT
                     AVAEHAGFPLVVKPIAGVPGEGQSVLLRTDDIEAAWQRAVGAGTVAHNRVLAETVVEV
                     DFEITLITVRTTGPTGPALHFCEPIGHREGDGDLLEAWQPQPLSAAALDAARSIAARI
                     VNALGGRGVFGVELLVRGDEVYFSDVRTRPHDSGLVTLRSQRLSEFELHARAILGLQV
                     DTIMISPGAAEVTYAGAESPVDIAPENVNAVLADALAVAESDARLFNRPDETEPRRRL
                     GLALATAPDVTTARDRTRRVTGVLRKLW"
     gene            complement(4953..5261)
                     /locus_tag="VGP27_05390"
     CDS             complement(4953..5261)
                     /locus_tag="VGP27_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV7850785.1"
                     /translation="MRKSSRGRDSKAPAAPKTCPHLLAATSEPEPVTPGECQDCADLG
                     ENTWAHLRMCMTCGHVGCCDSSPHRHASEHFQRTGHPVMRSVEPEEDWRWCYIDVRLG
                     "
BASE COUNT          883 a         1937 c         1710 g          923 t
ORIGIN      
        1 cgatgcggcg ccgggtcagc tcggcgatcg cggacaaccg ccgcgcaccg gcgatcgcct
       61 cctggcggac cccatcctcg atggcagcca ccagctcctc atcagaggcc gccgcgaaca
      121 tcgaaccgaa cataggttcg acgctaccca ccaccgccga ccggaaccag cagctcaacc
      181 cgtacctgtg gatgaaatta cgactgtgaa taactcagcc gggtcacgcg gattgcccta
      241 cgcccgccac ttcccaccaa aacttgacac ctgtaaagtt tgatcgcatg gacaacatct
      301 ccggtaggac catcgcgatc accggcgccg cccgcggcat cggattcgct gccgcgaagg
      361 cgttgttggt ccgcggtgcg cgggtggtca tcggcgaccg tgacgtcgcg ttcctggagt
      421 cggccgtcgc cgacctgacc aagctggggc cggtgtcggg ctacccgctc gacgtgaccg
      481 accgcgcgtc gttcgccacg ttcctcgaca aggcccgcac cgacggcggc ggccatatcg
      541 acgtgctcat caacaatgcc ggcgtgatgc cgatcggacc gttcctcgac cagtccgagc
      601 agtcgatccg gtcgtcgatc gaggtcaatc tctacggcgt gatcaccgga tgccagttgg
      661 cgctgcccga catgatcgcc cgccgctgcg gccacatcat caacatcgcc tcgctatcag
      721 gcctcatccc ggttccgggc caagtggtct acgtgggcac caaattcggc gtggtcggtc
      781 tgaccgcggc actcgccgac gaggtcgccc cgcatggcat cgacgtctcg gtcatcatgc
      841 cgccgttcac caacaccgaa ttgatctcgg gcaccaagac cggcggcatc aagccggtgg
      901 aacccgagga catcgcagcg gcgattatca agacactgga gaagccgaaa acccatgtgt
      961 cggtgccgac tccgctgcgg ttcaccgcac aggccgcgca gatgctgggc ccgcgtggcc
     1021 gccgctggct caacaaacgc ctcggtctcg acaccgtgtt catgaccttc gaccacgcga
     1081 agcgccagag ctacgagcag cgcgcgcagg ccgctcaggg cgtggtggaa tccccggaac
     1141 ggtaggcctc gatcgcatcg atccgcccga agaactcgtc gggcgtcatc ggctgatcgt
     1201 ccgccgtgcc gacgaggatc gtgtgcacga cttcggcctc ctcggcgacg tgcatccaga
     1261 tcgtcgtcac cggctccaag cccttgccga cctcggcgcc cgacggcggg tagaaccaga
     1321 tgacgaactc ctcgtccgac tgttcctcgt cgaaggtcca gccccgctcc gtaattcgcg
     1381 catcgaagct ctccaagtcc tcgaccagct ccacatcgtc gacgatgtcg tcgagtaccg
     1441 agtcgggaat ccagttcacg tcccgcttgg cccgccgctt gttgcgacgc gccttcttcg
     1501 cctctgccgc ccgagacacc cgggacacct agcccagcgc ctggtccaga tcgttgatca
     1561 gatcctcggt accttcgagc cccaccgaga ttcggaccac gccgtcgccg agaccgatcg
     1621 ctgcacgacc ttccggcccc atcgcccgat gggtcgtggt ggccgggtgg gtgatcagcg
     1681 acttggcgtc gcccagattg ttcgagatgt cgacgatgcg cagtttgtcc agcacctcga
     1741 aggcgcgctc cttgctgccg cccttcagtt cgaaggtgac aaccgttcca ccaccggtca
     1801 tttgacgctt cgcgaggtcg tactgcggat gcgactccag gaacgggtag cgcacccagc
     1861 ccacggcggg atggctctcg aggaactgcg cgacccgcac cgccgaatcg ttgctgtact
     1921 gcacccgcac cgccagcgtc tccaggccct tcaacaacac ccacgcgttg aacgggctca
     1981 gcgcaggacc ggtgtgccgc atcagtttct gcaccggctc gtcgatgtag gccgcctcgc
     2041 cgaggatcgc gccgccgagc acccggccct gcccgtcgat gtgtttggtg cccgagtaca
     2101 ccaccacgtc gacgccgagc gggagtccct gctgcaggat cggagtggcg aacacgttgt
     2161 ccaacacgac ttttgcgccg acaccgtgcg ccatctcgct gaccgcggcg atatccacca
     2221 gcgactgcat cgggttcgac ggggtctcga agaacaccgc ctgcgtcgga accgacagtg
     2281 cctcttccca ttgcgagaga tcctcgccgt cgacgaacac cgtctcgatg ccccaccgcg
     2341 gcaggatctc gttgcacacc acgaagcacg agccgaacag gcttctggcc gccaccaacc
     2401 ggtcgcccgc ggccagcagc gcacccaatg aggtgaacac cgcggccatc ccggtcgcag
     2461 tcgcgaacgc cgcaggcgct ccttcgatca gccgcaaccg ctcctcgaac atcgagaccg
     2521 tcgggttgcc gtaccgcgag tacacatacc ggtcgatctc accggtgaac gccttctccg
     2581 cctcggcggc acttgcgtac acgtagcccg aggtcgggta gatcgcctcg gcggtctctt
     2641 cgaagcccga ccgcagcagg cccccgcgca ctccgatggt ggcctggctg acgccttccg
     2701 gcagttttgc gggcgtgcgc accgacggag tgtccgggtt gttgctcacg actgcttcca
     2761 cggcaggccg acggccttcc aaccggtggc cccgcggtgc ttgttctcgt cgaggtcgcc
     2821 ttcgaatccg tccaggatgt tgtacgacgg cgcgatgccc gcttcggtgg ccgcctcggc
     2881 cgcgccgatc gaacgattgc cggagcggca gaggaacacc acgggccgtc ccccaccggc
     2941 gtctggaccc acgcccggtt cgacgccggc cgcgacgagg tcgtcgacga aaccgtcgtt
     3001 ccggttgccg tcggtgcggt tccactcgac gtagatgacg tcgcgaccga gacccgtcag
     3061 gtcgggcaca ccgacgaacc gccattcggc gtcggtgcgg cagtcgacga gcacggcctg
     3121 cgggtcatcg ttgagcattt tccaggcctc ttcaggcgtg atgtctcccg catagctcac
     3181 gatcgcgagt gtcccatacc taactgggac cggtcgaaca gaccttccac gccccgtcct
     3241 cgcgcacgaa ggtcaccgga acaggcgcct tcgcatccgg cgccttgtcg aagtggtatg
     3301 tcacggtcgc cgtcgcgcgg tcgccgtcga tcttgatgtc ggtgacgtcg tcgacgaacc
     3361 gggcgccacg cttgacgact gaatcgcgtt gatgcgcaat cacatcggct tccacgccgc
     3421 gcatcttggc gcacatgtag gtgccgaagt cggcgaagtc ctcccgctgc agcgcgtcgt
     3481 tctgtccgac ggcggtgcgg ccgaccagtt ggtcctgcgg cgtgcggtcc gcccccaaca
     3541 cgttgaacaa ccagatggcg atcaccacga tcacaatgag tgccagcgcg ccaaggaacg
     3601 gggcgatcgt cggcctgtcg gcggcctcgt cgtcggccgg cacagcctcg tcgtcggacg
     3661 gcacggttac cagagtttgc gtagtacgcc ggtcactcgc cgcgtcctgt ccctggcggt
     3721 ggtgacgtcc ggcgcggtgg ccagcgcgag gccgagtcgg cgccggggtt cggtctcgtc
     3781 gggccggttg aacagccggg cgtcgctttc ggcgacggcc aaggcatcgg ccagcaccgc
     3841 attgacgttc tccggcgcga tgtcgaccgg cgactcggcg cccgcatacg tcacctctgc
     3901 ggcaccggga gagatcatga tggtgtccac ctgcagcccc aggatcgccc gagcgtgcag
     3961 ttcgaactct gaaagccgtt gcgaccgaag ggttaccagg ccgctgtcat ggggtctggt
     4021 gcggacgtcg gagaagtaga cctcatcgcc gcgcaccagc agttcgacgc cgaagacgcc
     4081 acgaccgccg agcgcgttga cgatgcgcgc ggcgatcgac ctcgccgcgt ccagcgcggc
     4141 cgccgacaag ggctggggct gccacgcctc cagcaggtca ccgtccccct cgcggtggcc
     4201 gatcggttcg cagaagtgca gcgcgggacc cgtcggcccg gtggtgcgga cagtgatcag
     4261 ggtgatctcg aagtcgacct cgacgaccgt ctcggcgagc acccggttgt gcgccaccgt
     4321 gccggccccc accgcgcgct gccaggccgc ctcgatgtcg tcggtgcgca gcagcaccga
     4381 ctggccctca ccgggcaccc ctgcgatcgg cttgaccacc agcgggaagc cggcatgttc
     4441 ggccacggcg gtcagctcgt cgaccgaccc ggcgaaccag aacggcgcgg tcggcaggcc
     4501 cagttcgtcc gcggccatcc tgcgcatgcc ttcccggtcc tggctcatcc ggacaccgcg
     4561 cggggtcgga aacacctcga ggccgcgctc ggcggcggcc accagcgcgt ccaccgcgat
     4621 gacaccggat tcggcgacca cgtacagcgg gttcgcccgc tcgatgatcg cgctgagcgc
     4681 ctcggcatcg ttcatcttga ccaccgccgc gcggtcggcg acgccgtgcg cgggcgcgtt
     4741 cgcgtaccgg tcgacggcga cgacctcgcg acccaaccgc tggaacgcca gggtcagctc
     4801 gcggctcaac tcaccggagc cgagcagcat caccgctttt cggtcgtcgt ggtgctggcc
     4861 gggctcattg tcggtcggct cttcgtccgg tgtgtcctct ggaacttcag actctgtggt
     4921 ttcggtcatc gcccgtcaag actgccagat cttcacccga gccggacgtc gatgtagcac
     4981 catctccagt cctcttccgg ctcgacggat cgcatgacgg ggtgcccggt ccgttggaag
     5041 tgttcgctgg cgtgtcgatg cgggctcgag tcgcagcagc cgacgtgacc gcacgtcatg
     5101 cacatccgca gatgcgccca tgtgttctcg cccagatcgg cacaatcctg gcattctccg
     5161 ggagtaacgg gctcgggttc ggaggttgca gccagcagat gagggcacgt cttgggcgct
     5221 gcgggcgcct tcgaatcgcg tccccgcgaa ctcttgcgca tgctgaccaa ggatagggac
     5281 attgaccgag gaggtctgac gaggtgcacg gctccctgct cgcggtgctg gtggcttcgg
     5341 tgctgctggc cgcattggcg aggcgatacg acgtgtcggc gccgctggcg ctggtggtcg
     5401 ccggcctggc ggcgagcaca attccggggt tcagcgacat cgacctcgat ccc
//
LOCUS       DASYLX010000223         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_338287,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000223 DASYLX010000000
VERSION     DASYLX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338287"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGP27_05395"
     CDS             complement(<1..414)
                     /locus_tag="VGP27_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV7850786.1"
                     /translation="MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSAAPGTLRTG
                     SRGCCSKEDAGDARRVADILDIPFYVWDFADKFSEDVIDDFVSSYARGETPNPCVRCN
                     ERIKFSALSARALALGFDAVATGHYARLTDGRLRRA"
     gene            complement(414..1676)
                     /locus_tag="VGP27_05400"
     CDS             complement(414..1676)
                     /locus_tag="VGP27_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV7850787.1"
                     /translation="MSPDTRPDALPASGSSRPDALPASGSSPVYLDHAATTPMHPAAI
                     EAMTAVLATVGNASSLHGAGRVARRRMEESRETLAKMLGARPSEVIFTAGGTESDNLA
                     VKGIYWARRDTEPRRRRIVTTAVEHHAVLDSVQWLAEHEGAEVTWLPTESDGSVAPGA
                     LRDALQQHDDVALVSVMWANNEVGTIMALPELASVAAEFGVPMHSDAVQAVPQIPVDF
                     AGSGLSAMSIAAHKFGGPTGVGALLLRRDTACVPLLHGGGQERDVRSGTADVAGAVAM
                     AAAAEITISGMATTSTRVQVLRDRLVDGVLSGIDDVVLNGATGAQRLPGNAHFTFRGC
                     EGDSLLMLLDARGIECSTGSACTAGVAQPSHVLTAMGADPASARGSLRLSLGHTSVDA
                     DVDAALDVLPAAVDRARQAAMASSGAVE"
     gene            complement(1724..>2392)
                     /locus_tag="VGP27_05405"
     CDS             complement(1724..>2392)
                     /locus_tag="VGP27_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7850788.1"
                     /translation="PLPGRSHIQRGYCRMMLRALGVRITVSGGPIRNLRGVLVVSGHV
                     SWVDIFSIGAVMPGSFVAKAELISWPALGFAARLMKVIPIERASLRRLPDVVGMVATR
                     LRAGQTVVAFPEGTTWCGLAYGAFRPAMFQAAVDAGRPVQPLRLRYHHRDGTPSTVAA
                     YVGDDTLIASIRRLVTARRTVVHVQVESLQLPGTNRRDLAARCEAAVRGDADRRTHGP
                     ALVA"
BASE COUNT          386 a          843 c          828 g          335 t
ORIGIN      
        1 ggcgcgccgc aacctaccat cggtgagccg cgcgtagtga ccggtcgcga cggcgtcgaa
       61 tccgagcgcg agcgcacggg ccgacagtgc agagaacttg atccgctcgt tgcaccgcac
      121 acaggggttc ggcgtctcgc cccgggcata ggacgacacg aagtcgtcga tgacgtcctc
      181 ggagaacttg tcggcgaaat cccatacata gaaagggatg tcgaggatat cggcgacgcg
      241 gcgagcgtcg cccgcgtcct ccttcgagca gcatccccgc gacccggtgc gaagcgttcc
      301 cggcgcggcc gacaacgcga ggtggacgcc gaccacgtcg tgtccggcat cgaccatccg
      361 ggcggcggcg accgacgagt cgacgccccc gctcatcgcg acaaggacgc gcactattcg
      421 acggctcccg aactcgccat cgccgcctgg cgcgcgcgat ccaccgcggc gggcagtaca
      481 tcgagggcgg cgtcgacgtc ggcgtcgaca ctggtgtgac cgagcgagag tcgcagcgat
      541 ccgcgggcac tggccgggtc ggcgcccatc gccgtcagca cgtgcgaggg ctgcgcgacg
      601 cctgccgtgc acgccgagcc cgtcgagcac tcgattcctc tggcgtccaa aagcatcagc
      661 agcgaatcgc cctcgcagcc gcggaaggtg aagtgggcgt tgccgggaag ccgttgcgcg
      721 cccgtcgcgc cgttgagcac cacgtcgtcg atgccggaca gcaccccgtc gaccagacgg
      781 tcgcgcagca cctgcacacg cgtgctggtg gtcgccatac cgcttatcgt gatttcggcg
      841 gctgccgcca tcgccaccgc acctgcgaca tccgctgtgc ccgaacggac gtcgcgttcc
      901 tgtccgccac cgtgcagaag cggcacacag gcggtgtcgc ggcgaagaag cagcgccccg
      961 acaccggtcg ggccgccgaa cttgtgagcg gcgatgctca tggccgacag gccgctgccg
     1021 gcgaagtcga ccggtatctg agggaccgcc tgtacggcgt cgctgtgcat gggcacgccg
     1081 aattccgcgg cgaccgatgc caactccggc agcgccatga tggtgccgac ctcgttgttg
     1141 gcccacatca ccgagaccag agcgacatcg tcgtgctgct gcagagcgtc ccgcagtgcg
     1201 ccgggtgcga ccgagccgtc ggattcggtc ggcaaccagg tcacctcggc gccttcgtgt
     1261 tcggcgagcc attggacgga gtcgagcacc gcgtgatgct cgacggcggt ggtgacgatg
     1321 cgccgacgcc gcggttcggt atcgcggcgc gcccagtaga tgcccttgac ggccaggttg
     1381 tcgctctcgg tgccgcccgc ggtgaagatc acctcggacg gacgcgcgcc aagcatcttg
     1441 gccagcgtct cccgcgactc ctccatgcgg cgccgcgcca cgcgtccggc gccgtgcagc
     1501 gacgatgcgt taccgaccgt cgccagcaca gctgtcatcg cctcgatggc ggcggggtgc
     1561 atcggggtgg tggcggcgtg gtccaggtag accggcgatg atccgcttgc gggaagagca
     1621 tcaggccgcg atgatccgct tgcgggaaga gcatcgggcc gagtgtcagg ggacataacc
     1681 gttcaaggat agccgccgcc atgcggccgc cctgatgccc tggtcaggcg accagcgcgg
     1741 gcccgtgtgt ccgtcggtcc gcgtcccccc ggaccgccgc ctcgcagcgc gccgccaggt
     1801 cgcgccggtt cgtacccggc aactgcagcg actcgacctg gacgtgcacg acggtgcggc
     1861 gtgcggtgac caatcggcgg atggacgcga tcagcgtgtc gtcccccacg taagcggcca
     1921 ccgtcgacgg tgtgccgtca cggtggtggt accgcagccg tagcggctgc accggccgtc
     1981 ccgcgtcgac ggcggcctgg aacatcgcgg gccggaacgc tccgtaggcc aggccgcacc
     2041 aagtcgtgcc ttcggggaag gcgacgacgg tctgcccggc gcgcagtcgg gtggcgacca
     2101 tcccgacgac gtcgggcaac cggcgcaggc tcgcccgttc gatcggaatg accttcatca
     2161 ggcgggccgc gaaacccagg gcaggccagc tgatcaactc ggccttggcg acgaacgaac
     2221 cgggcatcac cgcgccgatg gagaagatgt cgacccacga cacgtgcccg ctgacgacca
     2281 gcacgccgcg caggtttcga atcgggccgc ccgacaccgt gatccgtacg ccgagcgccc
     2341 gcagcatcat ccggcaatac ccgcgctgga tgtgcgagcg gccgggcagc gg
//
LOCUS       DASYLX010000224         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_448264,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000224 DASYLX010000000
VERSION     DASYLX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448264"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGP27_05410"
     CDS             complement(<1..618)
                     /locus_tag="VGP27_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7850789.1"
                     /translation="MVSVVQAQTAAAYSRQGLPVEYLEVPSAAMGRDVKVEFQGGGPH
                     AVYLLDGLRAQDDASGWDVNTSAFEWFYQSGISVVMPVGGQSSFYSDWYQPAAGSSGT
                     QTYKWETFLTQELPTYLQANKGQDPSNNAVVGLSMSGGAAMTLAIWHPAQFIFAGSLS
                     GFLNPSQGLWPTLIGLSMKDAGGFNAVDMWGTTSDPAWRRNDPMVN"
     gene            complement(827..1129)
                     /locus_tag="VGP27_05415"
     CDS             complement(827..1129)
                     /locus_tag="VGP27_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850790.1"
                     /translation="MKRFIGSALIGSALAAAALSLAGPAHAESAALTIGQLEAQGFDV
                     KLDRVGSAPLNKCEVIDVRNPRERTELVHVGGRGDHDFVPVVAARTITVSLDCSRR"
     gene            1316..2272
                     /locus_tag="VGP27_05420"
     CDS             1316..2272
                     /locus_tag="VGP27_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEV7850791.1"
                     /translation="MKTVVFGCVAALGLSLAAGVASADTMLGPPVVSTTFGLDTGPMP
                     DVASDPATHRAYVTSQGDDTLYVLDATSRIAAITVGPSPRFAAVDSTTQTAYVTTGDS
                     VSVVDIASGSVTGTIAVGTNPEGAEVDPDTRRLFIANAGDDSVSVVDTDSRTVTATID
                     VGSRPGKMGVDPTSHLLYVSNRGSATVSVVDPSLSSVIDTIAVGRNPNDVAIDPPTHT
                     AFVPNTADNSVSVVDLNSRAVIQTIAVGQQPSAGTVDSDAHTVLVTNHGAASVSVIDS
                     QRRVVTDTVGVGDGPQTVAADPATHTAWVVDFRANSISVIER"
     gene            complement(2310..>2753)
                     /locus_tag="VGP27_05425"
     CDS             complement(2310..>2753)
                     /locus_tag="VGP27_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7850792.1"
                     /translation="ALREAGALSPRLMELVRLRIAFHNQCRSCMSIRYQSAIDDGLTE
                     DAVCSLERPAEASDLTDPERSALRFADLFATDHLAIGDSVYDDLRRHFTEDQLVALGL
                     HCALCVGLGRLAATWHVVDDLPDEYRADSGTPLAPWSANSVVASG"
BASE COUNT          508 a          927 c          865 g          453 t
ORIGIN      
        1 gttgaccatc ggatcgttgc ggcgccaggc cgggtcgctg gtggttcccc acatgtccac
       61 ggcgttgaag ccgcctgcgt ctttcatcga gaggccgatg agcgtcggcc acaaaccctg
      121 tgaggggttg aggaagcccg ataacgagcc ggcgaagatg aactgcgccg gatgccagat
      181 ggcgagcgtc atggccgcac cgcccgacat cgacagtccc acaacggcat tgttggacgg
      241 gtcctgaccc ttgttggcct gaaggtacgt gggcaattcc tgtgtcagga acgtctccca
      301 cttgtatgtc tgcgtgcccg agcttcctgc tgccggctga taccagtcgg agtagaaact
      361 ggactgaccg ccgacgggca tgacgacgga gatccccgac tggtagaacc attcgaacgc
      421 cgaggtgttg acgtcccagc cactcgcgtc atcctgggcg cgtagaccat ccagcaggta
      481 cacagcgtgc gggccaccgc cttggaactc aaccttgacg tcgcgcccca tagcagccga
      541 cggaacctcc agatactcca ccggcaggcc ctgccgcgag taggccgcag cggtctgagc
      601 ctgaaccaca ctgaccatca gagccagcat cgcgaccgat accgccgcga ccgcatatga
      661 ccacgcactt aacgtcttgt cgaccacgtg ttccgccacc aacaccgcca cacctcatct
      721 gtctctggct cgccttcgcg caaggtagca acggcacatg acagacgcgg cgctgcagcg
      781 ccgcgcgtga tcggcttgtt atcaacacgt tccgagattg tgaggtctat cgacgtgaac
      841 agtcgagcga caccgtgatg gtccgcgcgg cgaccaccgg gacgaagtcg tggtcaccgc
      901 ggccgcccac atgcacgagt tccgtacgct cccgcggatt gcgtacgtcg atcacctcgc
      961 acttattcag cggcgcgctg ccgacacgat cgagcttgac gtcgaagccc tgcgcctcga
     1021 gttgaccgat ggtcagggcg gccgactcgg cgtgcgccgg gccggccagt gacagcgccg
     1081 cggcggcgag cgccgatccg atgagcgctg aaccgatgaa ccttttcatg aggaaaccct
     1141 tgttcgtgcg agccgacgct cgcgcttgaa tgacgagttc atggtgctgc gggccgacgg
     1201 acccgcgctt ggaggccgtg tggagattcg gtggagattg ccggtagatc ccgatggata
     1261 gccgctgagc tggcagactc accgccggca gggtcgtcga tggtgggggt tcgtcgtgaa
     1321 gacagttgtc ttcggttgcg ttgctgcgct gggcctgagc ctggctgcag gggtcgcgtc
     1381 ggccgacacg atgctgggac cgccggtggt ctcgacaacg ttcggcctcg acaccggccc
     1441 gatgcccgac gtcgcatccg acccggccac ccacagggcg tacgtcacca gccaaggcga
     1501 cgacacgctc tacgtcctcg acgccacttc gcgcatagca gccatcacgg tcggaccgtc
     1561 gccccggttc gccgccgtgg actcgacgac gcaaaccgcc tatgtcacga ccggcgactc
     1621 ggtatccgtc gtcgacatcg cgtcggggtc ggtcaccgga acgatcgccg taggaacgaa
     1681 tcccgagggc gccgaggtcg atccagacac ccgcaggctc ttcatcgcca acgcgggaga
     1741 tgattcggtg tccgtcgtcg acaccgactc ccgaaccgtc accgcgacga tcgacgtggg
     1801 cagtcggccg ggcaagatgg gcgtcgaccc gacaagtcat ctcctctatg tctccaatcg
     1861 cgggagcgcc accgtctcgg tcgtcgaccc ctcgctgtcg agcgtcatcg acacgatcgc
     1921 cgtgggccgc aatccgaacg atgtggccat cgatccgccg acgcatacag ccttcgttcc
     1981 gaacacagcc gacaactcgg tgtccgtggt cgacctcaac agccgtgccg tcatccagac
     2041 gatcgccgta ggacagcagc cgtcggcggg cacggtcgac tccgacgccc acacggttct
     2101 ggtcacgaac cacggtgcgg catcggtgtc agtgatcgac tctcagaggc gtgtcgtcac
     2161 cgataccgtc ggagtcggcg acgggccgca aaccgtcgcc gccgacccgg cgacgcacac
     2221 cgcatgggtg gtcgacttca gggccaactc gatctccgtc atcgagcgtt agcccggacc
     2281 cgttgggcag acaccttcaa gctaaagatt cagcccgacg cgacaacgga attcgcgctc
     2341 cacggcgcga gcggggttcc gctgtcggcg cgatattcgt cgggcagatc atcgaccaca
     2401 tgccaagtcg cagcgaggcg cccaagcccc acgcacagcg cgcagtgaag gcccaaggcg
     2461 accaactgat cctcagtgaa atgccggcgc aggtcgtcat acaccgagtc gccgatcgcg
     2521 agatggtcgg tggcgaacag gtcagcgaac cgcaatgcgc tgcgctcggg atccgtcagg
     2581 tccgacgctt cggcgggtcg ctcgagtgag cacaccgcgt cctcggtgag gccgtcgtcg
     2641 atcgcgctct ggtagcggat cgacatacag ctgcggcact ggttgtgaaa ggcgatgcgc
     2701 agccggacaa gctccatcag ccgcggcgag agggcgcccg cctcgcgcag cgc
//
LOCUS       DASYLX010000225         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2203566,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000225 DASYLX010000000
VERSION     DASYLX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203566"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGP27_05430"
     CDS             complement(<1..591)
                     /locus_tag="VGP27_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV7850793.1"
                     /translation="MVLMKAADDRLSKGIGTGEFMCVYWPSRGQEAIAAAMGVTLRDH
                     DQLVTTYRGLHDLIGKGVPLEEIYGEMMGRTVGASRGKGGTMHIAKPDKGVMLSTGIV
                     GAGPPVGVGLALAAKRKGLDRVTVVSFGDGATNTGSFHEAANMAALWDLPMVFLCQNN
                     MYAEMTPTTDTMKLEHVADRAAGYGMPGVRVDGNDPL"
     gene            complement(630..938)
                     /locus_tag="VGP27_05435"
     CDS             complement(630..938)
                     /locus_tag="VGP27_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850794.1"
                     /translation="MARGIIYVETMPASPDREAEYHKWYNDTHLEQILSVDGIVSARR
                     FAPTSGAGPFVAIYELDCDDLDAAAQQMGGLALTGLDNIAMDPKPIPHVFREIGSLGP
                     "
     gene            complement(952..1812)
                     /locus_tag="VGP27_05440"
     CDS             complement(952..1812)
                     /locus_tag="VGP27_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7850795.1"
                     /translation="MGRVEGKVALISGAARGQGRSHAQLLAAEGADIIAVDICEDIET
                     NEYPLARPEDLDETARLVEKEGKRAHAEIADVRDRAALSAAIDRGVAEFGRLDIVVAN
                     AGICPLTAGLPPQAFADAVDVDLLGVLNLVHASLKHLQAGASIVVTGSNAAFMSSMNT
                     TGIDGGPGGAGYAFAKLAGAHYVNDFALALAPFSIRMNAIHPTNVNTDMLHSPPMYRA
                     FRPDLKEPTREEAELVFPVVQAMPVPYVEPEDISEAVVFFASDAARYITGQQLRVDAG
                     GFLKVKPWSA"
     gene            1933..3090
                     /locus_tag="VGP27_05445"
     CDS             1933..3090
                     /locus_tag="VGP27_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850796.1"
                     /translation="MDFSYPAEVEQFRKELRTWLAAHLTDDVRSADRVRGRDPAAFET
                     LRAWDAAVADAGWGAVSWPQEYGGRGATVLEQLVYAEETTRARAPVPLNVIGMNNIAP
                     AIMQYGTDSQRRTLLPRMARADDIWCQGMSEPEAGSDLASLRTRAVRDGDDFVVNGQK
                     IWTSLGHRADWCQLYVRSDPEAAKHKGISCLIVDMRLPGIEARPLVTLNGAADFAEVF
                     FNDVRVPADALLGPLNAGWQVATTTLSHERAGAARLYAQMEVQLADLVADLAGHTVDG
                     RPVLADRATLVKLGEVAVRINYLELLCKRSISATLHGGDALGSASLAKSVWAELGQEL
                     AELAFDVLGGDKAGQRWVDRRLSSRSLTIAGGTTQINKGITATRVLGLPRR"
     gene            3170..>3393
                     /locus_tag="VGP27_05450"
     CDS             3170..>3393
                     /locus_tag="VGP27_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850797.1"
                     /translation="MNLELTEEQIALRDTVRQFLAEKASVAAHVRPLLDDPTGTTDVV
                     WRGLADLGATGVLVPSDHDGAGMTMVEAGIV"
BASE COUNT          565 a         1138 c         1109 g          581 t
ORIGIN      
        1 cagtggatcg ttgccgtcga cgcgcacgcc gggcatgccg tagcccgccg cgcggtcggc
       61 gacatgctcg agcttcatcg tgtcggtggt cggggtcatc tcggcataca tgttgttctg
      121 gcagaggaag accatcggca gatcccacaa cgcagccatg ttggcggcct cgtggaacga
      181 gccggtgttc gtcgcgccgt caccgaaact gacgacagtg acccggtcga gtcccttacg
      241 cttggcggcc agcgccagcc cgacgcccac cggcggcccc gcgccgacga ttcccgtcga
      301 cagcatcacg cccttatcgg gtttggcgat gtgcatcgtg ccacccttgc cgcggctggc
      361 ccccaccgtg cggcccatca tctcgccgta gatctcctcc agcggtacgc ctttgccgat
      421 caggtcgtgc agcccgcggt aggtcgtcac caactggtcg tggtcacgca gggtcacacc
      481 catagcggcg gcaatggcct cctgcccgcg cgacggccag tacacgcaca tgaactcgcc
      541 ggtgccgatg cccttggaca gccggtcgtc ggcggccttc atcaacacca tcaacgcata
      601 gatgcgccgt tgcacctctg ccgtaagcgt cacggtccga gtgacccgat ctcgcggaac
      661 acatggggaa tgggcttggg gtccatggcg atgttgtcga ggcctgtcag cgccagtccg
      721 cccatctgct gcgcggccgc gtcgaggtcg tcgcagtcga gctcgtagat cgcgacgaac
      781 gggccggcgc cgctcgtcgg cgcaaaccgg cgcgccgaga cgatgccgtc caccgacagg
      841 atctgctcca gatgcgtgtc gttgtaccac ttgtggtact cggcttccct gtccggggac
      901 gccggcatcg tctccacgta gatgatccct ctggccatcc tcgctactcc tttacgccga
      961 ccacggtttc actttgagga acccacccgc gtccactcgc agctgctgac cggtgatgta
     1021 tcttgctgcg tcactggcga agaacaccac cgcctcgctg atgtcctcgg gttctacgta
     1081 gggcaccggc atcgcctgca ccacagggaa aaccagttcg gcctcctcgc gggtcggctc
     1141 cttcaggtcg ggtcgaaacg cccggtacat cggcgggctg tgcagcatgt cggtgttgac
     1201 gttggtcggg tgtatcgcgt tcattcgaat cgagaacggc gcgagcgcaa gcgcgaagtc
     1261 gttgacatag tgggcaccgg ccaactttgc gaacgcgtag cccgcgccac cgggaccgcc
     1321 gtcgataccg gtggtgttca tcgatgacat gaaagccgcg ttcgatcccg tcacgacgat
     1381 cgacgcgccc gcctgcagat gcttcaggct cgcatgcacg aggttgagca cgccgagaag
     1441 gtccacgtcg acggcgtcgg cgaacgcctg cggcggcaga cccgccgtca gcggacagat
     1501 acctgcgttc gcgacgacga tgtcgagcct gccgaactcg gcgaccccgc ggtcgatcgc
     1561 cgccgacagc gcggcgcggt cgcgcacatc ggcgatctcg gcgtgcgccc gcttaccttc
     1621 tttctccacc aagcgggcgg tctcgtcgag atcctcgggc cgggcgagag ggtactcgtt
     1681 ggtctcgatg tcctcgcaga tgtcgacggc gatgatgtcg gctccctcgg cggcgagcag
     1741 ctgtgcgtgc gatcgaccct gacctcgcgc cgccccgctg atcagcgcaa ccttgccctc
     1801 gactctgccc atcgtcgccc tccttgactg tccacacgag ccggattggg aaccttcgag
     1861 tcacctcgca gagatcgcta tactacctaa aattataaag atgccaatcg gcccgatctc
     1921 gaacaggaca ccgtggactt ctcatatccg gcggaggtgg agcagttccg caaggagttg
     1981 cgcacctggc tcgcggcgca cctgaccgat gacgtcagga gtgccgaccg ggtccgcggg
     2041 cgcgaccctg ctgccttcga gacgcttcgt gcgtgggatg cggcggtggc cgacgcgggc
     2101 tggggtgcgg tgtcctggcc gcaggaatac ggtggccgcg gcgcgacggt tttggagcaa
     2161 ttggtctacg ccgaggagac gacgcgggcc cgagccccgg tgccgctcaa cgtgatcgga
     2221 atgaacaaca ttgccccggc gatcatgcaa tacgggacgg attcgcagag gcgaacactg
     2281 ctcccccgca tggcgcgcgc cgacgacatc tggtgtcagg gcatgtcaga gcccgaggcc
     2341 ggctctgatc tggcgtcgct gcgcacccgc gccgtccgcg acggcgacga tttcgtggtc
     2401 aacggccaga agatatggac gtcgctgggt caccgcgccg actggtgtca gctgtacgtg
     2461 cgctccgacc ccgaagccgc caagcacaaa ggcatctcct gtctcatcgt cgacatgaga
     2521 ctgcccggca tcgaagcccg cccgcttgtc acgctcaatg gcgcggccga cttcgccgag
     2581 gtgttcttca acgacgtccg cgtgcccgcc gacgcgctgc tcggcccgtt gaacgcggga
     2641 tggcaggtcg ccaccacgac actgagccac gagcgtgcgg gcgccgcccg gttgtacgcg
     2701 cagatggaag tccagttggc cgacctcgtc gcagacctgg ccgggcacac agtggacggc
     2761 aggccggtac tcgccgaccg cgcgacgctg gtcaaactcg gtgaagtcgc ggtgcggatc
     2821 aactatctcg aattgctgtg caagcggtcg atctcggcga cgctgcacgg cggtgacgcc
     2881 ctcggctcgg ccagcttggc caagagtgtg tgggccgagt tgggacagga actcgccgag
     2941 ctggccttcg acgtgctcgg cggcgataag gccggccaac gctgggtgga ccgccgtctg
     3001 tcgtcgcgct cgctgaccat cgcgggcgga acgacgcaga tcaacaaggg catcacggcc
     3061 acgcgtgtcc tggggttgcc gcgacgatga gcgcttgcgc gaagtggggg cactccccta
     3121 gcaagctgtg ggcccacccg cccgaagggc agggggacag aaggcagcga tgaacctgga
     3181 actcaccgaa gagcagatcg cgctccgcga tacggtgcgg cagttcctcg ccgaaaaggc
     3241 gagcgtggca gcacatgtcc ggccactgct ggacgatccg acgggtacca ccgacgtggt
     3301 ctggcgtggt ctggcggatc tcggcgcaac gggcgtgctc gtcccgtcgg accacgacgg
     3361 tgcgggcatg acaatggtcg aggccggcat cgt
//
LOCUS       DASYLX010000226         6858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1546127,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000226 DASYLX010000000
VERSION     DASYLX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546127"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..120
                     /locus_tag="VGP27_05455"
     tRNA            44..120
                     /locus_tag="VGP27_05455"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Asp,seq:gtc)
     gene            200..273
                     /locus_tag="VGP27_05460"
     tRNA            200..273
                     /locus_tag="VGP27_05460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Phe,seq:gaa)
     gene            584..1228
                     /locus_tag="VGP27_05465"
                     /pseudo
     CDS             584..1228
                     /locus_tag="VGP27_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1578..2471
                     /locus_tag="VGP27_05470"
     CDS             1578..2471
                     /locus_tag="VGP27_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEV7850798.1"
                     /translation="MASMAAGGEGTGLRPDFENVQAHYDLSDEFFRLFLDPTQTYSCA
                     YFERDDMTLEQAQIAKIDLALGKLGLQPGMTLLDIGCGWGATLRRAVEKYDVNVVGLT
                     LSRNQAIAVDKLFREMDSTRSRRVYLAGWEEFHEPVDRIVSIGAFEHFGKARYDDFFT
                     MAHSVLPPDGVMLLHTIVRASLEEVQRRGLKLPAKLLKFILFIMDEIFPGGELPLVAE
                     VQQHALKAHFQVARVQPLQPHYARTLDIWAATLQSHREQAIAVQSEEVYQRYMKYLTG
                     CADLFRDGYTDVCQFTLVKNG"
     gene            complement(2608..2823)
                     /locus_tag="VGP27_05475"
     CDS             complement(2608..2823)
                     /locus_tag="VGP27_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850799.1"
                     /translation="MAFTVYLSADEGIHHRYGDKDRYELSAAGTLVVRPADGTETYTY
                     AQWVYLKADTDHTSGGPKSGSGKATFI"
     gene            3084..3458
                     /locus_tag="VGP27_05480"
     CDS             3084..3458
                     /locus_tag="VGP27_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7850800.1"
                     /translation="MSKDQNIEILNKGYAAFSSGDAEGAMRDLDDNIEWVEPGNSAIS
                     GTYNGKAEVGEFFAKLSEKSFTTTPNRFIADDDEVVVLTEVKVGGESARSADLYTFRD
                     GKVVRAENFSDTAMMERVFGRK"
     gene            complement(3625..4797)
                     /locus_tag="VGP27_05485"
     CDS             complement(3625..4797)
                     /locus_tag="VGP27_05485"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV7850801.1"
                     /translation="MAITNVAEYAHLSVAELEALAADLEGIRRDVEQSLGAKDRAYIH
                     RAIAFQRGLEVASRLVIGLSKGKVGWVLGATALAAAKSIENMELAHNIGHGQWDWMND
                     PEIHSVTWEWDMVGPSSQWRYSHNYRHHVFTNVVGMDEDLGYGVMRVTRDQTWKPSNL
                     TQPLRNLLMAVTFEWGIGLHDLYSEKLRATTDADRSTHTKAFVRKIARQAAKDYVLYP
                     SLSGRLWRRTATANVTANLMRNVWAYVVICCGHFADGAQKFNTAALVDETKPEWYLRQ
                     MLGTANFNAGPVLAFMSGNLCYQIEHHLFPDLPSNRYADIARRVKAVCVAYDLPYTTG
                     PLVRQYLLALRTIMKLALPDRFLTATSDDAPETASEGRFRQIENARPSKTTSQRAR"
     gene            5022..5285
                     /locus_tag="VGP27_05490"
     CDS             5022..5285
                     /locus_tag="VGP27_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850802.1"
                     /translation="MEPESEEIPDFSPFSKIRRIALDPESVVGKELVENFEQLDESFK
                     GKNKSWSAIVKLIALSAMCVKGVIELEKQVIEMRRQVRSRAEM"
     gene            5340..5924
                     /locus_tag="VGP27_05495"
     CDS             5340..5924
                     /locus_tag="VGP27_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850803.1"
                     /translation="MGELTPEQFTHLVLLAAAPPDRVGEVVASLVGEHLTIGPLEVGP
                     GGVASATARGTRGEVRVDKCVDDYWRQIITVPIALSVDVRLAGQIVRYRGEVEVQIRF
                     RLRLDHPCAVTVALEQVREQHVRTTVDPVGLGARLIGWIGGVDEIVAEQVLCYVRDLM
                     SSPEFDAAMHIDVNGLMKRAWDAGLVVALPARSS"
     gene            complement(5990..6238)
                     /locus_tag="VGP27_05500"
                     /pseudo
     CDS             complement(5990..6238)
                     /locus_tag="VGP27_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
BASE COUNT         1392 a         2052 c         2160 g         1254 t
ORIGIN      
        1 gggggtacac gcttcgcgta acgcggagaa agatgtactg caaggccctg tggcgcagtt
       61 ggttagcgcg ccgccctgtc acggcggagg tcgcgggttc gagtcccgtc agggtcgcca
      121 cggcagcgag gcacgtcgcg cacgcgagga gcgagaacgc atcgcgcacg cgagaagcga
      181 gaacgcatcg gtgccgccgg gccaggtagc tcagttggta cgagcgtccg cctgaaaagc
      241 ggaaggtcgc cggttcgatc ccggccctgg ccacttcaat ttgtgcagct cagtcacctt
      301 atcgggagat ctcacggact tgcttccttt tgggatggcg tgttgatggg gccaatatgg
      361 ggccaaaacg accagctgct gcgatggcgg atgcgaatga gtccaagggg gaagcgtcga
      421 tcaccggtta gccgcttcga gcatggtggc gttccgatcg ttgaagcgca actacgagga
      481 cggccaagat cgcgtcgagt agcaccagtg atgcaatcgg tggcagccat agggcggcga
      541 tgccagcagc acacagtgcg aaacatgcga tgagacgttc agcgggttcg aagtgaactt
      601 tctccctgag tacaaggcct tacacgatgc cggttacaac gtgctggcat atgacatccg
      661 caaccacggt atgagcggac aaggcaacgg ggggatcgcg gggattggcc tcaccgagta
      721 ccgcgacgtc atcggatcgc tgcgctacgc cgcggcgcgc ccggatacca aagacatgaa
      781 gaaggcgctg ctatcggtct gcctcggggc tgattccacc gcggtcgcat ggtccaaaca
      841 tccggaagag ttctccgagg ttcaggcgat ggtcatgctg cagcctgtct ccggaaagta
      901 catcgtcgaa gagttcgtca agggcatcgg catggaggac ggatacgaga agttcgatgc
      961 tctcgtccat gagcgcactg gatttcacct cgctgagcag tcgccgctag aacacgtgag
     1021 ggcggtcacg gttcctacgc tcgttgctca ggtgcacgac gacaccatga cccggcccca
     1081 agacgtgcaa gacatctacg acgctatccc cgtcgaagag aagagcctgt actggattag
     1141 aggaaccgac aaacgattcg agggatacaa cttcttcggg gtccatcccg aactcccgat
     1201 cgaatggttc gacaaacacg tcaactgagg ccccatatcg ctgagctgaa gcccgagccg
     1261 tctcaaccag gctgacaacc aagttccgga acgggctgtc taattgttgt caagaatccc
     1321 ggttgagatc atccagcgag tcgagcgcgg cggcgatgtt atcgagctcg tcatcgaaaa
     1381 gatcggcgtg tgcccaaaaa cccgctcacg ttggcacgct tcggattgcc cacggtgctt
     1441 cccgcatcga catttgccaa ggtcttccgc acagcgagtc agccggaatt gtcgcggttt
     1501 gtcacaactc gcgcgaacct ctgcgtcgct gtgctcaacc tattgcgtcg acgcgggccg
     1561 gcgcataatc tcgcgccatg gcctcgatgg ctgcgggcgg ggaggggacg ggcctgaggc
     1621 cggatttcga gaatgtccag gcgcactatg acctgtccga tgagttcttc cggctcttcc
     1681 tcgatccgac ccagacctac agctgcgcct atttcgagcg cgacgacatg actctggagc
     1741 aggcccagat cgccaagatc gacttggcat taggcaagct agggctgcag ccgggcatga
     1801 cgctgcttga catcggctgc ggctggggtg ccacgttgcg gcgtgccgta gagaaatacg
     1861 acgtcaacgt cgtcgggctg accttgagca ggaaccaggc catcgccgtt gacaagttgt
     1921 tccgcgagat ggacagcacg cgcagccggc gggtgtactt ggcgggctgg gaggagtttc
     1981 acgaaccggt cgaccggatc gtgtcgatcg gtgcgttcga acacttcggc aaggctcgct
     2041 atgacgactt tttcaccatg gcgcactccg tgctgcctcc cgatggcgtg atgctgctgc
     2101 atacgatcgt cagggccagc ctcgaagagg tccagaggcg gggcctgaag ctaccggcga
     2161 aactgctgaa gttcatcttg ttcatcatgg atgagatctt tccgggcggg gagctgccct
     2221 tggtcgccga agttcagcaa cacgcgctca aagcccattt tcaggtggcc cgcgtccagc
     2281 cgctgcagcc ccactacgcc cgaacgttgg acatctgggc ggcgacgctg cagagccacc
     2341 gagagcaggc cattgcggtt cagtccgaag aggtctacca gcgctacatg aagtacctca
     2401 ccggttgcgc cgacctgttc cgtgacggct acaccgacgt atgccagttc actttggtga
     2461 aaaacggctg accgactgtg gccggctcag cccaggaacg cgttcggccg tctgcgagtc
     2521 cgtcggctta cgcccacgca ggttcgaatc ctgcacctgc caccaggtca gagcccccgt
     2581 cggcggccag ggcggccgct tgacgcgcta gatgaacgtc gctttcccgg agccggactt
     2641 cggaccaccc gaagtgtggt cggtatcagc cttcaaatag acccattggg catacgtgta
     2701 tgtctcggtc ccgtccgcag gtctgacaac caacgtgccc gcggcggaga gctcgtacct
     2761 gtccttatct ccgtaccggt ggtgaatgcc ctcgtctgcg gagaggtaca cggtgaatgc
     2821 catagttgga gcgtgccatg agcgcgggac cgaaacccgc cggtgaccac agtgatttgg
     2881 ctcttaggtc tcgcgctccg gttctggccg gttcgcggcg tgctgcgagc agtccaaaat
     2941 tgtcagagct ggcaattacg gaatgcggcc agatctgctc atttaacgcc gtcagcaagc
     3001 gcccagccgt actctgacgc cgtctcaaca ggccgaccgc ctccaactgt ggttggcgcg
     3061 agaccccatc gaagaaggtc aagatgtcaa aagatcagaa catcgagata ttgaacaagg
     3121 gctacgccgc gttctcctcc ggagacgccg aaggggctat gcgggacctc gatgacaaca
     3181 tcgagtgggt cgaaccgggc aacagcgcga tcagcggcac gtataacggc aaagccgaag
     3241 tgggtgagtt cttcgcgaag ctgtcggaga agtcgttcac aaccacccct aaccggttca
     3301 tcgccgacga cgacgaggtg gtcgtgctga ccgaagtcaa ggtaggcggc gaatcggctc
     3361 ggagcgccga tttgtacacc ttccgcgacg ggaaagtcgt aagagctgaa aacttctcgg
     3421 acacagccat gatggagcgg gtcttcggca gaaagtaagc ggtcgcttcg actcgcgggc
     3481 gcacagggga cgaggtattc gctctgatct gtcccggcgg ccaaagcctc ggtgcgacgt
     3541 caggaaacca aaccgataac cacagccttc gtcgggacga tgacggacac gatccgctgc
     3601 attcgccgcc atatcagtcg gtcttcagcg cgctcgctgc gacgtcgtct tcgacggtcg
     3661 agcgttttcg atctggcgga acctgccctc ggacgctgtc tcaggtgcgt cgtcggaagt
     3721 ggcagtgagg aaacgatccg gaagggccag cttcatgatc gtccgcagcg cgagcaggta
     3781 ttggcggacc agcggaccgg tcgtgtaggg cagatcgtag gcgacgcaca cagctttcac
     3841 ccgccgggcg atgtccgcgt aacggttgct gggaagatcg ggaaagaggt gatgttcgat
     3901 ctgatagcag aggttcccgc tcatgaacgc caataccggc cccgcgttga agttcgccgt
     3961 acccagcatt tggcgcaagt accattctgg cttcgtctcg tcgaccagtg cggcagtgtt
     4021 gaatttctgg gcgccgtccg cgaagtgtcc gcagcagatc accacgtagg cccaaacgtt
     4081 gcgcatcaag ttggccgtca cgttggctgt ggcggtacgc cgccacagtc ggccgctgag
     4141 cgacggatag agtacgtagt ccttcgcggc ctggcgggcg attttgcgga cgaaggcctt
     4201 ggtgtgagtt gatctgtcgg catcggttgt tgcgcgcaac ttctcggagt acaggtcgtg
     4261 cagcccgatg ccccattcaa acgtgaccgc catcaacagg tttcgtagcg gctgcgtcag
     4321 attacttggc ttccacgtct ggtcgcgggt gactcgcatg actccatatc cgaggtcctc
     4381 gtccatgccg acaacgttgg tgaagacgtg gtggcggtag ttgtgggagt agcgccactg
     4441 cgacgacggg ccgaccatgt cccactccca ggtaactgag tggatttccg ggtcgttcat
     4501 ccagtcccat tgaccgtggc cgatgttgtg agcgagttcc atgttctcga tgctcttcgc
     4561 cgcggccagc gctgtggctc ccagcaccca tccgaccttg cccttgctca agccgatcac
     4621 gaggcgcgag gcgacttcca ggccacgctg aaatgcgatc gcgcgatgaa tatatgcccg
     4681 gtctttcgcg cctagcgact gctcgacatc acggcggatt ccctccaggt cggcggcgag
     4741 tgcctccagc tctgcgacgc tgagatgagc gtattcagcg acattggtga tggccatcgt
     4801 ccgtcaaacc ctttcaggcg cgatttgcgt ccccgacttg cgcaatctcg acaagcacag
     4861 ccgctgtgaa cgactcccac gggctgaagg agtgaagcta cgcattctcc actaccccac
     4921 aacgagcgac aaaaaaccag acgggccctg gtggagatgg aagctccggg tcgaagacga
     4981 gatcgagccg ggaggcccag cgaccgggct aggctagcaa tatggagccc gagtccgaag
     5041 agatcccaga tttctcgccg ttcagcaaaa tcagacggat agctcttgat cccgaatcgg
     5101 tggtcggtaa ggaacttgtc gaaaatttcg agcagcttga cgagtccttc aagggcaaga
     5161 acaagagttg gtctgcgatc gtgaagctca tcgcgctgag cgcgatgtgc gtcaaggggg
     5221 tgatcgaact ggaaaagcag gtgatcgaaa tgaggcgcca ggtgcgctcc cgcgccgaaa
     5281 tgtgatgcgc taagcgcaaa ctgaacgtcg agtcaccgtc gagaggacag ggaaggccct
     5341 tgggtgagtt aacgcccgaa cagttcactc acctcgtgct gctcgcggcg gcgccgccgg
     5401 accgggtcgg cgaggtggtg gcgagcctag ttggcgaaca tctcaccatc ggccctcttg
     5461 aagtcggccc gggaggggtc gcgtcggcaa cggcgcgggg gacgcgcggt gaggtccgcg
     5521 tcgacaaatg tgttgacgac tactggcgcc aaatcatcac cgtcccaatc gcgctgtcgg
     5581 tcgacgtgcg gttagcggga caaatcgtcc gctatcgcgg cgaagttgag gtccagatac
     5641 gatttcggtt gcgtctcgac cacccctgcg cggtgactgt tgcgctcgaa caggtgcgcg
     5701 aacaacatgt ccgtacaacc gttgatccgg tcggcctcgg tgctcgcctc atcggctgga
     5761 ttggcggagt agacgaaatc gtcgccgagc aggtgctgtg ttacgtacga gatctgatgt
     5821 ccagtcccga gttcgacgcg gccatgcaca tagacgtgaa tggactgatg aagcgcgcat
     5881 gggacgccgg tctggtcgtc gcgctgcctg cacgctcgtc ttgaacggtt catgatttca
     5941 gttgccacgc caccaacggg tcggacgtgg acagattgag cggtgcgaag ttgagcgcgg
     6001 cgcgcaggcc gtcgagtacg cagtatccag gttccaccgg cgcgccgaaa acgcccttca
     6061 ggccgccgtg gtccgggagc gacaccgccc agccctcggc gagtacggcg gcgacgagca
     6121 gcaattcgaa ctgtgccagc gcgtcagcgg ccttggcacg gcggcgcaac gcgtaggacg
     6181 gaaagcagcg gtcggacacg gcgtcgattg cgcactggta cgacacgatc ggacaggggg
     6241 cagtcggtgt gtcggatctc catgccaggg cagcgctaac agttgccgaa tactgcgtcg
     6301 tccgctgagt gaccacgacg cgcacccgat gtactggttc gccaccgaca tgggcgtgcc
     6361 aggaccatcg accccgctgg tcaccggagt gttacgcgac atcgccgcag actctgacgc
     6421 gacccagacg ctactgcagg tcctcaatca cgaggtcaag ccgacaggct tgctaacccc
     6481 aaggcggctg gccatcgccg cggcacgagc gcttcgcgac cgccctgacc acatcggcgc
     6541 gacgagcaag gaaatcgctg ccgcgctgtg caacgaaatc cgccgcgcac gctggacccg
     6601 caagacaccg cccggtatga acggcggccc acaaatgagc cgcgtacaac gcgcggctcg
     6661 cagcgagaat ctgaggaggg cggccggccg cggcgccgca caacacgaag ccatggggcc
     6721 aaaatggggc caaaccgcca atccgagcgc tccagccggt ccactcgcgt gctgggaccc
     6781 aactcggcca tctgaaacac attgatgtca gccaaaactc cgcctgaaaa gccgaaggtc
     6841 gccggttcga tcccggcc
//
LOCUS       DASYLX010000227         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1216000,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000227 DASYLX010000000
VERSION     DASYLX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216000"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGP27_05505"
     CDS             complement(<1..881)
                     /locus_tag="VGP27_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV7850804.1"
                     /translation="MPTNAKVPPAKLARVVEWTRHHLYRLHQRLVPAPAAMMEMIIAT
                     WTSQAITVAAQLGVADALADGPLTIDELAVRVDADADALRRLLRALISRGIFRQRRDG
                     RFELNSLADTLRSDAPASMSSAARFYGSREQRERWTVMVDAVRTGNSVVPALRGKSSF
                     DYFAEQPELAELFNQTMTSVSEMTDLTVVAGYDFSAYPTIVDVGGGHGPLLAAVLAAA
                     PASRGVLYDLPSVVADAPNLLREHGVADRVRIADGSFFDSVPGGGDAYILKNIIHDWP
                     DEKALQVLRNVRAAAGR"
     gene            complement(886..2142)
                     /locus_tag="VGP27_05510"
     CDS             complement(886..2142)
                     /locus_tag="VGP27_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850805.1"
                     /translation="MAARLGDSDGRFAKHKPAPDALRILVAGAGVAGISIARGLLRDG
                     HDVTVFEQRPDMRPGGGAVTIWSNGSTVLDQLGVDMDGAGQLLSTVRISTSTGRPLVN
                     IDVAAIVDRLGGSVRMVPRRVLLDRLLEGFPAERIRCNTRAVGAVSTQDGVRIEFEDG
                     TFADADLLIGADGLHSKIRGVAGAKPAKPTGWCTWQGLITLPHIAEKDVAVQIIGPHG
                     NLGLWPAGGSDLQWWFDLPWSPGFVRPERPIDMIRSNFAGWSEMVDQVLAALTDDDLA
                     PSPFPHFRHPVPGSPRLDAVTLLGDAAHTMPPILAQGTNQALLDTMVLRKALADFCNG
                     GNGDLSSALRRYEKTRRRRLSAVSWVTSRQMSQSETVLKPAAMISDSFGTWAMTRFLR
                     WLSHRRISDEVSQGLSSPVIHACSEG"
     gene            complement(2148..>3034)
                     /locus_tag="VGP27_05515"
     CDS             complement(2148..>3034)
                     /locus_tag="VGP27_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7850806.1"
                     /translation="ESVLGLEVVTADGERIYCDPENHPDMYWAARGSGPGFFGVVTSF
                     KLRIHPRPAVVGTCLYMYPIELADEIYTWAREISSEVDDRVELQILATRDNPAAGIDQ
                     PTITIASPVFAESDEEAAKALAVLGTCPVAGKAMINLPYMPTTLANWYTAVMTNYPEG
                     HRYIADNMFTSASAAELLPGIRKIIETMPPHPSHFIFTGWNASPDRADKVYGLEAEIY
                     MALYTVWQDPADDERYRDWAASSMAAMSHLATGVALADENLGRRPARFITEPNMARLD
                     KVRATYDPDGRFHRWMSC"
BASE COUNT          542 a          999 c          964 g          529 t
ORIGIN      
        1 tggcggccgg ccgccgcgcg aacattgcgc aggacttgca gcgccttctc gtcgggccag
       61 tcgtgaatga tgttcttgag gatgtaagcg tcacccccac ccgggacgct gtcgaagaac
      121 gatccgtcgg cgatccgaac ccggtcggcg acaccgtgct cccgcaacag gtttggtgca
      181 tccgcgacga ccgacggtag gtcgtagagg acgccccgcg atgcgggcgc cgccgccagg
      241 acggcggcca gcagtgggcc gtgcccgcca ccgacgtcga cgatggtcgg ataggcgctg
      301 aagtcgtagc cggcgacaac ggtcaaatcc gtcatctccg aaacactcgt catggtctgg
      361 ttgaacagtt cggcgagctc gggctgctcg gcgaagtagt cgaagctcga cttgccccgc
      421 aatgccggga cgaccgaatt cccggttcgg acagcatcca ccatcaccgt ccagcgctcg
      481 cgctgttccc ttgacccgta gaaccgtgcc gccgaactca tcgacgccgg ggcgtcggaa
      541 cgcagcgtgt cggcaaggga attcagctca aaccggccgt cgcggcgttg acggaagatg
      601 ccccggctga tcaacgcccg cagcaggcgg cgcagcgcgt ccgcgtccgc gtccacccgc
      661 acggccaact cgtcgatcgt caacggcccg tcggcgaggg cgtcggcgac accgagttgc
      721 gctgccacag tgatcgcctg cgacgtccag gtggcgatga tcatctccat catcgcggcc
      781 ggtgcaggca ccaaccgttg atggagccga tacaggtgat ggcgagtcca ctcgacaacg
      841 cgggccagct tggcgggtgg tactttggcg ttcgtcggca tcgcgtcagc cctcactgca
      901 cgcatggatc accgggctgg agaggccttg actcacttcg tcggatatcc ggcggtgact
      961 tagccaacgc agaaacctcg tcatcgccca agtaccaaac gagtcggaga tcatggcggc
     1021 cggtttcagc accgtttcac tctgggacat ctgccgcgac gtcacccagg acactgcgga
     1081 gagcctgcgt ctcctggtct tctcgtaccg gcgcagcgcg ctcgagaggt caccgttgcc
     1141 accgttgcag aaatccgcga gtgccttgcg caacaccatc gtgtcgagca gcgcctgatt
     1201 ggtcccctgc gcgaggatcg gcggcattgt gtgtgcggca tcgccaagca atgtcacagc
     1261 gtcaagacgc ggcgagccgg ggaccggatg ccgaaagtgc gggaacggcg atggcgccag
     1321 gtcgtcgtcg gtcaacgccg cgagtacttg atcgaccatc tcggaccagc ctgcgaaatt
     1381 ggaccggatc atgtcgatcg gacgttccgg cctgacaaag cccggcgacc acggcaagtc
     1441 gaaccaccac tgcaaatcgg agccacccgc cggccacagg ccgaggtttc cgtgcgggcc
     1501 gatgatctgc actgccacgt ccttttcggc gatgtgcggg agggtgatca atccctgcca
     1561 ggtgcaccag ccggtcggct tcgcaggttt tgcgcctgcg acacctcgga tcttcgaatg
     1621 caggccatcc gcaccgatca gtagatccgc atcggcgaag gtgccgtctt cgaactcgat
     1681 tcgcaccccg tcttgcgtac tgaccgcccc gaccgcacgc gtattgcatc ggatgcgttc
     1741 tgccggaaag ccttccagca gacggtcaag caggacccgg cgtggaacca tccgaacaga
     1801 tccgcccaac cggtccacga tcgcggcgac gtcgatattg accagcgggc gaccggtcga
     1861 cgtcgagatc cgcacggtgg acagcagctg acccgccccg tccatatcga cgcccagctg
     1921 atcgaggacg gtggaaccgt tggaccagat ggtcaccgcg ccgccgcctg ggcgcatatc
     1981 cggccgctgt tcgaagacgg tgacgtcgtg tccgtctcgc agtaatcccc gagcgatgga
     2041 gatacccgcc accccggcgc ccgcgacgag aattcgaagc gcgtccggtg ccggcttgtg
     2101 cttcgcgaat ctcccgtccg aatctcccag ccgcgcggcc attttcatca gcagctcatc
     2161 caacggtgaa atcgaccgtc tggatcatag gtcgcgcgca ctttgtcgag gcgcgccatg
     2221 ttcggctcgg tgatgaaccg tgccgggcgc cggccgaggt tctcatcggc gagcgcgacg
     2281 cccgtagcca ggtgcgacat cgccgccatg ctggacgcag cccaatcgcg gtagcgttcg
     2341 tcgtcggccg ggtcctgcca gacggtgtag agggccatgt agatctcggc ctccaagccg
     2401 tacaccttgt cggcgcgatc cggtgatgcg ttccaccccg tgaagatgaa gtgcgagggg
     2461 tgtggcggca ttgtttcgat gatcttgcgg atgcctggca gcagttcggc tgccgaagcc
     2521 gacgtgaaca tgttgtcggc gatgtagcga tgcccctccg ggtagttcgt catcacagcg
     2581 gtataccaat tcgcgagcgt ggtcggcata tagggcagat tgatcatcgc cttgcctgcc
     2641 accgggcagg tgccaagcac ggcaagggct ttcgccgcct cctcgtccga ttcggcgaac
     2701 accggcgagg cgatcgtgat cgtgggctgg tcgatcccgg ctgccgggtt atcacgcgtg
     2761 gcgaggatct gcagctcgac cctgtcgtcg acctccgaac tgatctcgcg cgcccaggtg
     2821 taaatctcgt cggcgagctc gatcgggtac atgtacaggc aggtgcccac caccgccggg
     2881 cgcgggtgaa ttcgcagttt gaacgaggtg accacaccga agaaacccgg tcccgagccg
     2941 cgggcggccc aatacatgtc ggggtggttc tcggggtcgc agtagatccg ttcaccgtct
     3001 gcagtgacga cctccagccc aagcacgctc tcgc
//
LOCUS       DASYLX010000228        14221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3740335,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000228 DASYLX010000000
VERSION     DASYLX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3740335"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1006)
                     /locus_tag="VGP27_05520"
     CDS             complement(2..1006)
                     /locus_tag="VGP27_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7850807.1"
                     /translation="MELKDTDTAPNVAGRPAPVPNPHATAEQVEAARHDSKLAQVLYH
                     DWEAESYDDKWSISYDKRCVDYARDLFDATVPPEEQRELPYDRALELGCGSGFFLLNL
                     IQAGVARRGSVTDLSPGMVKVATRNGQNLGLEIDGRVADAERIPYDDDTFDLVVGHAV
                     LHHIPDVELSLREVVRVLKPGGRFVFAGEPTNSGENYARPLSTLTWRAVTNVTRLPGL
                     SGWRRPQAELDESSRAAALEAIVDLHTFSPGDLEKMARSAGAVDIVTTTTEFTAAMLG
                     WPIRTFEAAMPPGRLGWHWAKFAFGSWMTLSWVDHNVWRRVVPKGWFYNVMVTGVKPS
                     "
     gene            complement(1017..1667)
                     /locus_tag="VGP27_05525"
     CDS             complement(1017..1667)
                     /locus_tag="VGP27_05525"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7850808.1"
                     /translation="MREFVSIHTFDGHPGIATLLLSRPPTNGLTRQMYREIIDAAGEV
                     AARDDIAAVIVFGGHEIFSAGDDVAVLQTLNAQEAAGAAQVGRDALDAIGAIPKPTVA
                     AITGYALGGGLTLALAADWRVAGDNIKVGATEILSGFPPGETARLARAIGESKAKDLV
                     FTGRFVDAREALALGLIDEMVAPDAVYDAAVAWAQRTVEHPPQVIAAAKATFGDGR"
     gene            complement(1664..2461)
                     /locus_tag="VGP27_05530"
     CDS             complement(1664..2461)
                     /locus_tag="VGP27_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7850809.1"
                     /translation="MSEDPLVPRPAATVMLIRDTPDGIKVFLMRRHRKMDFVGGVMVF
                     PGGGVDDRDRNADIAWHGPDKQWWGDKLGTDADLAEALVCAAARETFEECGVLFAGAA
                     DDPDLLVDDASSYRDQRAALVSKELSFAEFLRAEELMLRSDLLRPWANWVTPKEERTR
                     RYDTYFFVGALPEGQRADGDNTESDQADWITPEAALEDFTAQRSVLLPPTWTQLDALA
                     GRTVAEVLAVERQIIAVSPNMSMTDGNLEFEFFDGERYNQALGGRIP"
     gene            complement(2461..3327)
                     /locus_tag="VGP27_05535"
     CDS             complement(2461..3327)
                     /locus_tag="VGP27_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7850810.1"
                     /translation="MNTRHYDERVPDSGSDSVDPDLLIDFAKVTLRRGGQVLVGPVTW
                     SVELDERWVVIGPNGAGKTSLLRIAAATEHPSSGTAYVLGERLGRTDMAELRSRVGLS
                     SSALSQRVPDGELVRDLVVSAGYAVLGRWREQYQDVDYSQALDMLESVGAEHLAERNY
                     ETLSEGERKRVLIARSLMTDPELLLLDEPAAGLDLGGREELVARLEDLAADPDAPAIV
                     LVTHHVEEIPHGFSHALILSEGRVVDAGLLTDVLTAENLSRAFGQSIALDIIDGRYFA
                     RRTRSRAAHRRR"
     gene            complement(3367..4035)
                     /locus_tag="VGP27_05540"
     CDS             complement(3367..4035)
                     /locus_tag="VGP27_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEV7850811.1"
                     /translation="MTTGSDTLQRLAQDAIALAENRQRVILGVAGSPGAGKSTLVEQL
                     LARIGECKGPGWVTHVPMDGFHLADEQLRRLGALGRKGAADTFDSVGYALLLERIAAE
                     TDAPVYAPGFDRTLEQPLAAALVVPPTARLVITEGNYLLLDHPAWLRARRAMDRVWFV
                     TSEEDRRVERLVARHVQFGKSPDAAKAWVAGTDQPNADLVSPTAASADMVIVNGSDGW
                     LISP"
     gene            complement(4041..4940)
                     /locus_tag="VGP27_05545"
     CDS             complement(4041..4940)
                     /locus_tag="VGP27_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7850812.1"
                     /translation="MTSTAEVSNPIGVHALVWVGDTSPASVESAISQTVAAGYDILEF
                     SLHDAVNIDRVKTRELLTANGIKAACSRGLAREADISSEDTSVVARGAALLAESLAVT
                     HDIGATTLTGALYSAFGKAPCALTQTGRANVVAVLRDLAAEAAPLGVTLGLEICNRYE
                     TNVVNTARDCLRLADDIGADNIVIHLDTYHMNIEEDDMVTPVRDCGDRLGYVHIGENH
                     RGYLGSGHIDFAAFFGALAEIDYRGPLTFESFSSAVVAPGLSNDLAIWRNLWDDGFDL
                     ASHARRFISDGLRDARRHAAAPA"
     gene            complement(4937..5755)
                     /locus_tag="VGP27_05550"
     CDS             complement(4937..5755)
                     /locus_tag="VGP27_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7850813.1"
                     /translation="MTQATTHRPLAEDVPDDALLKIDGATLAFGQVRALKGVTMHARR
                     GEVTAIVGDNGAGKSTLIRCVSGVHAPDSGSIAFDGQAVHFRSPHEARDAGIETVHQN
                     LALVEDLTVWQNLFLNREIVRRFGPFAVLNRRAMRAGAQEMVSGLAVNVPAVGSRVRR
                     LSGGQRQAVSICRAAGFTSKLIIMDEPTAALGVQETARVEELITRLRSEGHAIVLISH
                     NFSQVLKLSDAVWVMRAGHCVAGRRTSDTDGDEIVALLTGARPGDYADQQGANA"
     gene            complement(5752..6747)
                     /locus_tag="VGP27_05555"
     CDS             complement(5752..6747)
                     /locus_tag="VGP27_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850814.1"
                     /translation="MTTAPIAPARDDDRRAEAPQSPGWRAVLDPANLATWAAPIALVL
                     LAIVFQALNPTFLSLGNLTSMLTSSAILIVLAIGQTFVVATAGIDLSIASAMTLAAVI
                     FGIAFDAGLLAAVVAAVVTGLAVGLVNGLIIARGRITDFIVTLGMLSAASGMALIFAD
                     GKPKTIISAPLLKLSTGTVWIFGYSFLIAIALATVAHVVLFHTRFGTYVLATGGNSEA
                     AAATGVPTARIKTRVYIIAGLTAGIAAILLVARVGAAEPASNTSFLLNSVAAVVLGGV
                     SLFGGRATIAGPVVGALLLTGLVNGLTLLGVSQFYQPLSVGVVVVAAAFLTRFQK"
     gene            complement(6744..7766)
                     /locus_tag="VGP27_05560"
     CDS             complement(6744..7766)
                     /locus_tag="VGP27_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7850815.1"
                     /translation="MSHSISPTRRLAVLAAVGGLGLALSACGSSTSGGGSGHVAAVVK
                     GLDNPFFQTMESGIKEEASAAGTEVTVQAANSITDTTGQADKLNGLAGQDFKCFIVNP
                     ITGTNLIQGIAQLSAKNVPIVNIDSPIDADAAKAANAKITTYIGTDNADAGAKAAAEM
                     GKLLPGGGTVALIGGTSGDVTSAARLDGFTKAVPANLKIISTVAADWERQMALTKATD
                     IMTANPTLSAFFVANDDMGLGVAQAIANRGKTGSVKVISVDGNKDAFEAVKSGGIDAV
                     VAQFPYVIGAMGVDACKAAIAGKTLPTNVKAPVQVVTKANVDAALASAPKPAAPYENP
                     FAELAK"
     gene            7896..9116
                     /locus_tag="VGP27_05565"
     CDS             7896..9116
                     /locus_tag="VGP27_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV7850816.1"
                     /translation="MQEVYVPAVGEADPSAGNGSPGHVLDLIRRAGGLSRADIIARTG
                     LSRSTVAARLDALQAAGWISSGQTTTARGRPPSHFHIRSDGGALLIADAGATGVRTAV
                     TDLRGQIGHESRIPLDITVGPERWLAEVGEMFEDLLEKTGIAADFVRGIGVAVPGPVD
                     FASATVVSPPIMTGWDGYPIRSWFADRFACPVLVDNDANAMTLGEHCTNFADRDSLVM
                     VKVATGIGAGIIAHGLIYRGADGSAGDIGHIQISPPDNMPTDDSEPVCRCGNTGCIEA
                     YGGGWALLRDLRAAGREVSAISDIARLLESGDPLAVSLVRRAGRFIGVALSDVVSLLN
                     PSVVIVGGELAASAATLMAGIRESVYARSLPLATRRLQIVPARLGDGAGTVGLTSMLA
                     DHIFDIRRIDATLT"
     gene            complement(9027..9755)
                     /locus_tag="VGP27_05570"
     CDS             complement(9027..9755)
                     /locus_tag="VGP27_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850817.1"
                     /translation="MREATARGGALLLGLIGYGFSMAMMVRAGLGLDPWDVFHQGLAL
                     RTPMTIGIASAVVGVAVLLAWIPLRNRPGIGTVANVIVIAVTVDASLATLASPTSLPA
                     RIAMMVGAVVLNAISTVLYIGAGLGPGPRDGLMTGLVVRTGLSVRIVRTSIEATVLVI
                     GWLLGGTVGVGTVLYASGIGPLVQLVIRVTPNKVLAHSGWASVTGPSKARRDRLRERR
                     VDAADVEDVVGQHAGQTDRPCTVT"
     gene            complement(9748..9900)
                     /locus_tag="VGP27_05575"
     CDS             complement(9748..9900)
                     /locus_tag="VGP27_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850818.1"
                     /translation="MSINWTSKFARPLGRIFRPAQRIPVEFLDRDAERVRNEIELIRI
                     RFPHHA"
     gene            9965..11407
                     /locus_tag="VGP27_05580"
     CDS             9965..11407
                     /locus_tag="VGP27_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV7850819.1"
                     /translation="MTVEMRARALDADRLARELGNWRTSSRSGPAYYGLADAIRLLIV
                     DGRLPVGARLPSERAMADSLRISRTTVTAAFAQLREDGYLNARRGARSTTALPVAPAA
                     RTEPATPSTSLAAAALSAPAAAVSEAFVDAIHEVTPYLHKVGHELVGVDVLREAIAER
                     YCERGLSTDPDQVLVTTGALHAFNLVLTTYVQPGDRVLVEQPTYHGALTSITTAGARP
                     VPVAMTEDGWELDAIQAAVGQMSPSLAYLIPDNQNPTGFTMPAHDRKRLADIITGTRT
                     RTVIDETILDMWLDEAVPAPMAAAMTTRRDLVLTIGSMSKSFWGGLRVGWIRAERATI
                     ATIAALRPSVDMGTPVLEQFAAARLLARRDELLPERREILRVRREFLLSLLDRHLPDW
                     EPGPGVGGMSLWVRLPAPMSTALSAAASRMGLELPAGPRFGVDGTLERFVRVPYALPE
                     DELSAAIELLARAWHSVTGSVAPEPTTVVV"
     gene            complement(11413..12654)
                     /gene="serB"
                     /locus_tag="VGP27_05585"
     CDS             complement(11413..12654)
                     /gene="serB"
                     /locus_tag="VGP27_05585"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEV7850820.1"
                     /translation="MGSSRKRSSLLITVTGVDQPGVTSALFEVLSRHKVELLNVEQVV
                     IRGRLTLGVLVSGTVEIAGGRELSEQVEDAIHGVGLDVTIEPSGEMPVMRQPSTHTIV
                     VLGRPITAEAFGVVASEVAALGVNIDFIRGVSDYPVTGLELRVSVPPGGVYQQLQAAM
                     ARVAGNEGVDIALEDYSLARRAKRLIVFDVDSTLIQGEVIEMLADRVGAHAAVAEITE
                     AAMRGELDFAESLNRRVATLAGLPAEVLEEVGEQIELTPGARTTLRTLRRLGFHCGIV
                     SGGFRQVIEPLAHELEMDFVAANQLEIVDGKLTGRVVGPIIDRPGKAKALRDFAQQAG
                     VPMEQTVAVGDGANDIDMLAAAGLGVAFNAKPALREVADASLSNPYLDTVLFVLGITR
                     GEIEAADIVDGTLRRVEIPDD"
     gene            complement(12706..>14221)
                     /locus_tag="VGP27_05590"
     CDS             complement(12706..>14221)
                     /locus_tag="VGP27_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV7850821.1"
                     /translation="QYNQLFTMHGTVMLLFYATPIVFGFANLVLPLQIGAPDVAFPRL
                     NALSFWLFLFGAMIAIAGFITPGGAADFGWTAYTPLTDAIHSPGAGGDLWIMGLAVAG
                     LGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSILVLLAFPLLTAALFGLAAD
                     RHLGAHVYDPANGGVLLWQHLFWFFGHPEVYIIALPFFGIVTEIFPVFSRKPVFGYTT
                     LIYATISIGALSVAVWAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQL
                     TFETPMLFSVGFLVTFLLGGLSGVLLASPPLDFHVTDSYFVVAHFHYVLFGTIVFATY
                     AGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWVGDEGMPRRYADYLPTDG
                     FTTLNIVSTIGAFILGISTIPFAWNVFKSWRYGEPVTVDDPWGYGNSLEWATSCPPPR
                     HNFTELPRIRSERPAFELHYPHMVDRMRSEAHIGRSHGPEDGDVSRLDNENVRT"
BASE COUNT         2398 a         4724 c         4758 g         2341 t
ORIGIN      
        1 tctacgacgg cttgaccccg gtcaccatga cgttgtagaa ccagcccttc gggacgacgc
       61 gacgccacac gttgtggtcc acccagctca gcgtcatcca actgccgaac gcgaacttgg
      121 cccagtgcca gcccaaccgg ccgggcggca ttgccgcctc gaacgtgcgg atgggccacc
      181 ccagcatcgc ggcggtgaat tccgttgtgg tggtgacgat gtcgacagca ccggcagagc
      241 gggccatctt ttccaggtcg cccggcgaga acgtgtgcag atcgacgatg gcctccagcg
      301 cggccgcacg cgacgattcg tcgagctcgg cctgcgggcg ccgccagccg gaaagccccg
      361 gcagccgcgt cacgttcgtc accgcccgcc acgtcagcgt cgacaacggg cgcgcgtagt
      421 tctcgccgga gttcgtcggc tcaccggcga acacgaaccg gccgcctggc ttgagcacac
      481 gaaccacctc gcgcagcgac aactccacgt cggggatgtg gtgcagcacg gcgtggccga
      541 ccaccagatc gaaggtgtcg tcgtcgtacg ggatgcgctc ggcgtcggcg acgcggccgt
      601 cgatctcgag accgaggttc tggccgttgc gggtggccac cttgaccatg ccgggagaca
      661 ggtcggtgac cgagccgcgc cgcgcaacgc ctgcctggat caggttcagt aggaagaacc
      721 cgctgccgca gcccagctcc agcgcccggt cgtacggcaa ctcccgctgc tcctcgggcg
      781 gcacggtggc gtcgaacaga tcgcgcgcgt agtcgacaca gcgcttgtcg taggagatcg
      841 accacttgtc gtcgtagctc tcggcttccc agtcgtggta gagcacctgg gccagcttgg
      901 agtcgtggcg ggccgcctcc acctgctccg ccgtggcgtg cggattgggg accggagccg
      961 gccggccggc gacattcggc gcagtgtcgg tgtccttgag ttccatgcag ggcagcctaa
     1021 cggccgtcgc cgaaggtggc cttggcggcg gcgatgacct gcggcggatg ctcgaccgtc
     1081 cgctgcgccc aggccaccgc cgcgtcgtag accgcgtcgg gggccaccat ctcgtcgatc
     1141 aagccgagag ccagggcctc cctggcgtcg acgaaccggc ccgtgaacac cagatccttg
     1201 gccttgcttt cgccgatcgc gcgggccagc cgagccgtct cgccgggcgg gaacccggac
     1261 aagatctcgg tcgcgccgac cttgatgttg tcgcccgcca cccgccagtc cgctgccagg
     1321 gccagcgtca gcccgccgcc gagcgcgtat ccggtgatcg cggcgacggt cggtttgggg
     1381 atcgcgccga tggcgtcgag ggcatcgcga ccgacttggg cggccccggc tgcctcctgc
     1441 gcgttgaggg tctgcagaac cgccacgtcg tcgccggcgg agaagatctc atggccgccg
     1501 aacacgatca ccgccgcaat gtcgtcacgc gcagccactt cgcctgcagc atcgatgatt
     1561 tcgcgataca tctgacgggt caggccgttg gtcggcggcc tggacagcaa cagggtggcg
     1621 atgccgggat gcccgtcgaa ggtgtggatg ctgacgaact ccctcacggg atacggcccc
     1681 cgagcgcctg gttgtagcgc tcgccgtcga agaattcgaa ctcgaggttg ccgtcggtca
     1741 tgctcatgtt cggcgagacg gcgattattt gccgttcgac ggccagcacc tcggcgacag
     1801 tgcggccggc cagcgcgtcg agctgagtcc acgtcggggg cagcaggacg ctccgctgag
     1861 ccgtgaagtc ctcgagggcg gcttccggcg tgatccagtc ggcctgatcg gactcggtgt
     1921 tgtcgccgtc ggcccgctgg ccctctggaa gcgcaccgac aaagaagtag gtgtcgtagc
     1981 ggcgggtccg ctcttctttg ggagtcaccc agttcgccca cgggcgcagc aggtcggagc
     2041 gcagcatcag ttcctcggcg cgcaggaact cggcgaacga cagctccttg ctgacgagcg
     2101 cggcccgctg gtcgcggtag ctcgacgcgt cgtcgacgag caggtccgga tcgtcggccg
     2161 cgcccgcgaa cagcacaccg cactcctcga atgtctcccg cgccgcggcg cagacgagag
     2221 cctcggcgag gtcggcgtcg gtgccgagtt tgtcgcccca ccactgtttg tcggggccgt
     2281 gccaggcgat atcggcgttg cggtcgcggt cgtcgactcc accaccgggg aacaccatca
     2341 ctcccccgac gaaatccatc ttgcggtgcc gccgcatcag gaacaccttg atgccgtcag
     2401 gcgtgtcccg gatcagcatt acggtcgcgg cgggccgcgg aacgaggggg tcttcgctca
     2461 tcaacgcctc ctgtgggctg cccggcttct ggtgcgcctg gcgaaataac ggccgtcgat
     2521 gatgtcgagc gcgatcgact ggccgaacgc cctggacaga ttctccgcgg tcagcacgtc
     2581 cgtcaacagc ccggcgtcga ccacccgtcc ttccgacagg atcagcgcgt ggctgaaccc
     2641 gtgcgggatt tcctcgacgt ggtgagtgac aagcacgatc gccggggcgt ccgggtcggc
     2701 ggccagatcc tccagccgcg ccaccagctc ctcgcggcca ccgaggtcga gcccggctgc
     2761 gggctcgtcg agcagcagca gttccgggtc ggtcatcagg gagcgggcga tcagcacgcg
     2821 tttgcgttcg ccctctgaca gcgtctcgta gttgcgctcg gcgaggtgtt cggcgccgac
     2881 gctctccagc atgtcgaggg cctgggagta gtcgacgtcc tggtattgct cacgccagcg
     2941 gccgagcacg gcatatcccg ctgacacgac caggtcgcgc accagctccc catccggcac
     3001 gcgttgcgac agcgccgagc tgctcaaccc gacacgcgag cgcagctcgg ccatgtcagt
     3061 gcggcccagg cgttcgccga gcacataggc cgtacccgag gatggatgtt ccgtcgcggc
     3121 ggcaatccgc agcagcgacg tcttgcccgc gccgttcggt ccgatgacca cccagcgttc
     3181 gtcgagttcg acggaccagg tgaccgggcc gacgaggacc tgcccgccgc gccgcagtgt
     3241 gaccttggcg aaatcgatca gcaggtcggg gtcgacgctg tcgcttccgg agtcgggcac
     3301 tcgctcatcg tagtgacgcg tattcacgca ggattcggcg gcttcacgag cagacggccg
     3361 ccgccgtcat ggcgatatca gccaaccgtc tgacccgttc acgatcacca tgtccgcgga
     3421 ggccgccgtc ggcgacacca ggtcggcgtt gggctggtcg gtgccagcga cccaggcctt
     3481 cgcggcgtcg gggctcttgc cgaattggac gtggcgggcc accaaccgct cgacgcgccg
     3541 atcctcctcg ctggtgacga accacacgcg gtccatggcc cggcgcgctc ggagccatgc
     3601 cgggtggtcg agcagcagat agttgccctc ggtgatcacc agtcgcgcgg tcggcggcac
     3661 caccagtgcc gcggccagtg gctgttcgag ggtgcggtcg aatcccgggg cgtagaccgg
     3721 cgcatcggtt tccgcggcga tccgctcgag cagcagcgcg tagcccactg aatcgaaggt
     3781 gtcggctgcg cccttgcggc cgagtgcccc gaggcggcgc agctgttcat cggcgaggtg
     3841 gaagccgtcc atggggacgt gcgtgaccca cccgggtccc ttgcactcgc cgatgcgggc
     3901 aagtagctgc tcgaccagtg tggatttgcc cgctcccggg cttccggcga cgccgaggat
     3961 gacacgctgc cggttctcgg cgagcgcgat ggcgtcctgc gccagccgtt gcagggtgtc
     4021 ggatccggtg gtcacgcttg tcatgccggc gccgcggcgt gccgccgcgc gtcgcgcaat
     4081 ccgtcgctga tgaaccggcg ggcgtgcgag gccaggtcga aaccgtcgtc ccacaggttg
     4141 cgccatatcg caaggtcgtt cgacagcccg ggggcgacga cggccgacga gaacgactcg
     4201 aatgtcagcg gaccgcgata gtcgatctcg gcaagtgcac cgaagaacgc cgcaaagtcg
     4261 atgtgcccgg aacccaggta gccgcggtgg ttttcgccga tatggacgta gcccagccgg
     4321 tcaccgcagt cgcgcaccgg cgtgaccatg tcgtcctctt cgatgttcat gtggtacgtg
     4381 tcgaggtgga tgacgatgtt gtcggcaccg atgtcatcgg ccagtcgcaa gcagtcccgc
     4441 gctgtgttga ccacattcgt ctcgtagcgg ttgcagatct cgagcccgag cgtgacgccc
     4501 agaggcgcgg cttcggcggc aaggtcacga agtactgcga cgacgttggc ccgacctgtt
     4561 tgcgtcagcg cgcacggcgc cttcccgaac gcgctgtaga gggcgccggt cagcgtggtg
     4621 gcaccgatgt catgagtcac ggcaagagat tcggccaata gggccgcgcc acgtgcgaca
     4681 actgatgtgt cttcgctcga gatatcggct tctcgggcca gcccccgcga gcatgcggcc
     4741 ttgataccgt tggctgtcag cagttctcgt gtcttgaccc ggtcgatgtt caccgcgtcg
     4801 tgtaacgaga actcgaggat gtcgtatccc gcggcaacgg tctgcgagat agccgactcg
     4861 acgctggcgg ggcttgtgtc gcccacccac accagcgcgt ggacgccgat cgggttggaa
     4921 acctcggcgg tcgaagtcat gcgtttgctc cttgttgatc tgcgtagtcg cccggccgcg
     4981 caccggtgag cagcgcgacg atctcatcgc cgtcggtgtc ggaggtgcgc ctgcccgcca
     5041 cgcagtggcc ggcccgcatc acccacacgg cgtcggagag cttgagcacc tgcgagaagt
     5101 tgtggctgat caggacgatc gcgtgacctt ccgaacggag tcgcgtgatc aactcctcga
     5161 ccctggccgt ctcctggaca ccgagggccg cggtcggctc gtccatgatg atcaacttgg
     5221 acgtgaaacc ggcggcgcgg cagatggata cggcttggcg ttggccaccc gagaggcggc
     5281 gcacccgcga tccgaccgcg ggcacgttga ctgccaggcc ggacaccatc tcttgcgccc
     5341 cggcgcgcat cgcacgccgg ttcaggaccg cgaacggacc gaagcgccgg acgatctcac
     5401 gattcaaaaa caggttctgc cacacggtga gatcctcgac cagggccaag ttctgatgca
     5461 cggtctcgat gcccgcgtcg cgcgcctcgt gcggtgagcg gaagtgtacg gcctgcccgt
     5521 cgaaggcgat cgagcccgag tcgggggcgt gcacgccgct gacgcagcgg atcagtgtcg
     5581 acttcccggc accattgtcg ccgacaatgg cggtgacctc gccgcggcgc gcgtgcatcg
     5641 tcacgccctt gagcgcccga acctgaccga aagccaatgt ggcaccgtcg attttgagca
     5701 gggcatcgtc gggtacgtcc tcggcaaggg ggcggtgtgt ggtggcctgg gtcatttctg
     5761 gaacctcgtg aggaaggctg cggcgacaac aacgacgccg accgacagcg gttgatagaa
     5821 ctgcgaaacc cccagcagcg tcaggccgtt gacgagcccg gtgagcagca gtgcacccac
     5881 tacgggacct gcgatggtgg cccggccgcc gaacaggctg accccgccga gcacgaccgc
     5941 agccaccgag ttgagcagaa acgaggtgtt cgaagcgggc tcggcggcac cgacgcgggc
     6001 cacgagcagg atcgcggcga tacctgcggt caggccggcg atgatgtaca ccctggtttt
     6061 gatccgcgcg gtcggcaccc cggtcgcggc tgccgcctca ctgttaccgc cagtcgccag
     6121 cacgtaggtg ccgaagcggg tatggaacag caccacgtgg gcgacggtgg ccagcgcgat
     6181 cgcgatgagg aagctgtacc cgaagatcca cacggtgccc gtggacagtt tcagcagggg
     6241 cgcgctgatg atggtcttgg gtttgccgtc ggcgaagatc agcgccattc ccgacgccgc
     6301 agacaacatg cccaaggtga cgatgaagtc ggtgatccga ccccgcgcga tgatcagccc
     6361 gttgaccagt ccgaccgcga gcccggtcac gacggcggcg acgacggcgg ccagcagacc
     6421 ggcgtcgaac gcgatcccga agatcaccgc cgcgagcgtc atggccgatg cgatcgacag
     6481 gtcgattccg gcggtggcca ccacgaacgt ctgcccgatc gccagcacga tcaggatggc
     6541 cgacgacgtc agcatcgacg tcaggttgcc caggctcaga aacgtcggat tgagcgcctg
     6601 gaagacgatc gcgagcagca caagcgctat cggcgcggcc caggtcgcca agttcgcggg
     6661 atccagcaca gcccgccagc cgggcgattg cggcgcctcg gctcggcggt catcgtcgcg
     6721 ggccggcgca atgggggcag ttgtcatttg gcgagctccg cgaacgggtt ctcatagggt
     6781 gccgcgggct tgggcgccga ggccagtgcg gcatcgacgt tggccttcgt cacgacctgc
     6841 accggagctt tgacgttggt gggcaacgtc tttccggcga tggcggcctt gcacgcgtcg
     6901 acccccatgg cgccgatcac gtacgggaac tgcgccacaa cggcatcgat gccaccgctc
     6961 ttgacggcct cgaacgcgtc cttgttgccg tcaacgctga tgaccttgac cgaaccggtc
     7021 ttgcctcgat tggcgatcgc ctgggccaca ccgagcccca tgtcgtcgtt ggcgacgaag
     7081 aacgcgctca gcgtggggtt ggcggtcatg atgtcggttg ccttggtgag ggccatctgg
     7141 cgttcccagt cagcggccac ggtcgagatg atcttgaggt tggcgggcac tgccttggtg
     7201 aagccgtcaa gccgggcggc gctggtcacg tcgcccgagg tgccgccgat cagcgctacc
     7261 gtcccaccgc cgggcagcag tttgcccatt tcggccgcgg ccttagcgcc tgcgtcggcg
     7321 ttgtcggtgc cgatgtaggt ggtgatcttc gcgttggcgg ccttagcggc gtcggcgtcg
     7381 atggggctgt cgatgttgac gatcggaacg tttttggccg agagctgtgc gatgccctga
     7441 atcaggttgg tccccgtgat cggattgacg atgaagcatt tgaagtcctg gccggcgagg
     7501 ccgttgagtt tgtccgcctg cccggtggtg tccgtgatgg agttggcggc ctgcaccgtg
     7561 acctcggtac cggcggccga ggcctcctcc ttgattcccg actccatggt ttggaagaac
     7621 gggttgtcga gtcccttgac gacggcggcg acgtggcccg agccaccgcc ggacgtggag
     7681 ctcccacacg cgctgagcgc cagccctagg ccgccgacgg cggccagcac ggctaggcgg
     7741 cgggtggggg aaatcgaatg tgacatgcgt ctctcctctt cgagcggacc gagcgggtct
     7801 caactttctg cctaagtaga accatatata tgtctactgt caagcaaaaa tccgcgcaaa
     7861 tctggtaaaa agaatgggag gaaaggtggc cggccatgca agaggtgtac gttccggccg
     7921 ttggcgaggc agatccgtcc gcgggcaatg gatcgcccgg ccatgtgctg gacctcattc
     7981 gccgcgccgg cgggctgagt cgggccgaca tcatcgccag gaccggcctg tcccgcagca
     8041 ctgtcgccgc gcgcctggat gcgctgcagg ccgcgggatg gatctccagc gggcagacga
     8101 ccaccgcacg cgggcgcccc cccagccact ttcacattcg ctccgacgga ggagcgctgc
     8161 tgatcgctga cgcgggagcc accggtgttc gcaccgcggt cacggacctt cgtggccaga
     8221 tcggacacga gtcgcggata ccactcgata ttacggtcgg acccgaacga tggctggcgg
     8281 aggtcgggga aatgttcgaa gatctcctcg aaaagactgg catcgcagcg gatttcgttc
     8341 gcggaatcgg ggtcgccgtg cctggccctg tggacttcgc gagcgcgacg gtggtcagcc
     8401 cgcccatcat gaccggttgg gatggatatc cgatccggtc gtggttcgca gaccgattcg
     8461 cgtgcccggt gttggtggac aacgacgcca acgcgatgac gcttggcgag cactgcacga
     8521 acttcgccga ccgggattcg ctggtgatgg tcaaggtggc caccggtatc ggtgccggga
     8581 tcattgcgca cggcttgatc taccgcggtg ccgacggttc cgctggcgac atcggccaca
     8641 tccagatcag cccacccgac aacatgccga ccgatgattc cgaaccggtg tgccgctgcg
     8701 gaaacacggg atgcatcgag gcctacggag gcggatgggc gctgctacgc gacctgcgag
     8761 cagccggaag ggaggtgtcc gcgatctccg acatagcccg actgctcgaa tcgggtgacc
     8821 cattggcggt cagcctcgtt cgccgcgccg gtcgattcat cggcgtcgca ctgtccgacg
     8881 tggtgagcct gctcaaccca tcggtcgtga ttgtgggtgg tgaactggcc gcaagcgccg
     8941 ccaccttgat ggccggcatt cgggaaagcg tctacgcccg ctcattgcct ctggccactc
     9001 gtcgactcca gatcgtccca gcccggttag gtgacggtgc agggacggtc ggtttgacca
     9061 gcatgctggc cgaccacatc ttcgacatcc gccgcatcga cgcgacgctc acgtaatcga
     9121 tctcggcggg ctttcgaggg cccggtgacc gacgcccagc cgctgtgcgc cagcaccttg
     9181 ttcggggtga cccggatgac gagctggaca agaggtccga tcccggacgc gtacaagacg
     9241 gtgccgacgc cgacggtgcc gccgagcagc cagccgatca cgagcacggt ggcctctatc
     9301 gaggtccgca cgatccggac cgagaggccg gtgcgcacca ccagtcccgt catcagcccg
     9361 tcgcgtgggc ccggcccgag gcctgcgccg atgtagagca cggtactgat cgcgttcagc
     9421 acgaccgcgc cgaccatcat cgcgatacgg gccggaagcg atgtgggcga cgccaaggtc
     9481 gccagcgacg cgtccacggt caccgcgatg acgatgacgt tggccaccgt gccgataccg
     9541 gggcggttgc gcagcgggat ccaggccagc agcaccgcca ccccgaccac ggccgacgcg
     9601 atgccgatgg tcatcggtgt tctcaacgcg agcccctgat ggaacacgtc ccacgggtcc
     9661 agcccgagtc cggcgcgcac catcatcgcc atcgagaagc cgtagccgat gagcccgagc
     9721 agcagcgcgc cgccgcgggc cgtcgcctca cgcatggtgc ggaaatcgaa tccgaatcag
     9781 ctcgatctcg ttgcgcaccc gttcggcgtc gcggtccaga aactcgaccg ggatgcgctg
     9841 cgccggccgg aagatcctgc ccagtgggcg ggcgaatttt gatgtccaat taatgctcat
     9901 gacccaaata atgtcgccaa ctggcttgtt attccatagc cagtcggcgc atactggcct
     9961 gcaaatgaca gtagaaatgc gcgcccgagc gctcgatgcg gaccgtttgg cccgcgagct
    10021 tggcaactgg cgtacgtcca gtcgcagcgg gccggcctac tacggccttg ccgacgccat
    10081 ccggctgctg atcgtcgacg ggcgcttgcc cgtcggtgca cgcctgccca gcgaacgggc
    10141 gatggccgac tctctgcgta tctcccgcac caccgtcacc gccgccttcg cgcaactgcg
    10201 cgaggacggt tatttgaacg cacgccgcgg agcccgcagc accacggccc tacccgtcgc
    10261 gcccgccgcc cgcaccgaac ccgcgacacc gtcgacgagt cttgccgcag ccgcgttgtc
    10321 cgctcctgcg gccgccgtca gcgaggcgtt cgtcgatgcc atccacgagg tgacgcccta
    10381 tctgcacaag gtgggtcacg aactcgtcgg cgtcgacgtt ctgcgagaag ccatcgccga
    10441 aagatactgc gagcgagggc tttccaccga ccccgaccag gtgcttgtca ccaccggcgc
    10501 gttgcatgcc ttcaacctcg tgttgaccac atacgtgcaa cccggcgacc gtgtgttggt
    10561 ggaacagccc acctaccatg gcgcgttgac gtcgatcacc accgcggggg cgcggcccgt
    10621 gccggtagcc atgacggagg acggctggga actcgatgca attcaggccg cggtgggcca
    10681 aatgtcgccc agcctggcct atctgattcc ggacaaccag aatcccaccg gcttcacgat
    10741 gcccgctcac gaccggaagc ggttggcaga catcatcacc gggacgagga cgcgcacggt
    10801 catcgacgag acgatcctcg acatgtggct tgacgaagcg gttcccgcgc cgatggccgc
    10861 cgcgatgacg acgcggcgtg acctggtgct gacgatcggt tcgatgtcga agtcgttctg
    10921 gggcggcctg cgcgtcggct ggattcgcgc cgaacgcgcg accatcgcca ccatcgccgc
    10981 gctgcgcccg tccgtcgaca tgggtacgcc ggttctcgaa cagttcgccg ccgcaaggct
    11041 tctcgcgcgg cgggacgaac tgctgcctga gcgccgggag atactgcggg tacgacgcga
    11101 gttcctgctt tccctgcttg accggcatct gcccgactgg gaacctgggc cgggtgtcgg
    11161 cggcatgtcg ctgtgggtgc ggctaccggc accgatgagc acggcgctgt cggccgccgc
    11221 gtcccggatg gggctcgaac tgcctgccgg accacgattc ggcgtcgacg gcacactcga
    11281 acgcttcgtg cgagtgccct atgccctgcc ggaggacgag ctttcggcgg ccatcgagct
    11341 actggcccgc gcttggcaca gcgtcaccgg ctccgtcgcg cccgagccga cgaccgtcgt
    11401 ggtctgaccg agctagtcgt cggggatttc caccctgcgg agcgtgccgt cgacgatgtc
    11461 ggcggcctcg atctcgccgc gggtgatccc gaggacgaac agcacggtgt ccaggtacgg
    11521 gttgctcagc gacgcgtcgg ccacctctcg cagcgcaggc ttggcgttga acgcgactcc
    11581 cagaccggcc gcggccagca tgtcgatgtc gttggcgccg tcgccgacgg cgaccgtctg
    11641 ctccatcggc acacctgcct gttgcgcaaa gtccctgagg gctttggctt ttcccggccg
    11701 gtcgatgatc ggcccgacca cccgtcccgt cagcttgccg tcgacgatct cgagctggtt
    11761 ggcggccacg aagtccatct ccaactcgtg ggcgagcggc tcgatgacct ggcggaagcc
    11821 gcccgacacg atcccgcagt gaaaacctag gcggcgcaat gtccgcaggg tggtgcgcgc
    11881 gccgggcgtc agctcgatct gctccccgac ctcctcgagc acctcggccg gcaggccggc
    11941 cagcgtcgcg acgcggcggt tcagcgactc tgcgaaatcc agctcgcccc gcatggcggc
    12001 ctcggtgatc tcggcgacgg ccgcgtgtgc accgacccgg tctgccagca tctcgatgac
    12061 ctcgccctgg atcagcgtgg agtcgacgtc gaacacgatg agccgcttgg cccgccgcgc
    12121 gaggctgtag tcctcgagcg cgatgtcgac accctcgttg cccgcaaccc gcgccatcgc
    12181 ggcctgcaac tgctggtaga caccgccggg cggcaccgaa acccgcagct ccaatcctgt
    12241 gaccggatag tcggacacac cgcggatgaa gtcgatgttg acgccaagtg cggccacttc
    12301 actggccacc acaccgaacg cctcggcggt gatcggcctg cccagcacga cgatggtgtg
    12361 ggtggacggc tgacgcatca ccggcatctc gccgctgggc tcgatcgtca cgtcgagccc
    12421 gacaccgtgg atggcgtcct cgacttgttc gctcagctcg cgtccgcctg cgatctcgac
    12481 ggtgcccgat accagcacgc ccagcgtcag gcgcccacgg atgaccacct gttcgacgtt
    12541 gagcaactcg accttgtgcc gcgacagcac ctcgaacagc gcagaagtca cacccggttg
    12601 atcgactccg gtgacggtga tcagaagcga tgagcgcttg cgcgaagagc cgataggtga
    12661 agccaccttt cgactatcca ccctggctgg ccccccgcgc cggcatcagg ttctgacgtt
    12721 ctcgttgtcc agccgagaga cgtcgccgtc ctctggtccg tggctgcgcc caatgtgcgc
    12781 ttctgagcgc attcggtcca ccatgtgcgg gtaatgcagc tcgaaagccg ggcgctccga
    12841 acggattcgg ggcagctcgg tgaagttgtg ccgcggcggc gggcaactgg tggcccactc
    12901 cagtgagttg ccgtaacccc atgggtcgtc gacggtcacc ggctcgccgt agcgccagct
    12961 cttgaacacg ttccacgcga acgggatcgt cgagatgcca aggatgaacg cgccgatcgt
    13021 cgagacgatg ttcagcgtgg tgaacccgtc ggtcggcagg tagtcggcgt agcggcgggg
    13081 catgccctcg tcgccgaccc agtgctgtac caggaacgtg gtgtggaagc cgatgaacgt
    13141 cagccagaag tgcagcttgc cgaggcgctc gtcgagcagt cgccctgtca tcttcgggaa
    13201 ccagaaatag atgcccgcgt aggtggcgaa cacgatggtg ccgaacagca cgtagtggaa
    13261 gtgtgcgacg acgaaatagc tgtcggtgac gtggaagtcc agtggcgggc tggccaaaag
    13321 cacaccggac aggccaccga gcaggaaggt gaccaggaag cccaccgaga acagcatcgg
    13381 tgtttcgaat gtcaactggc ccttccacat ggtgccgatc cagttgaaga acttgatacc
    13441 ggtcggcacc gcgatcagga acgtcatgaa actgaagaac ggcagcagca ccgcgccggt
    13501 cgcatacatg tggtgcgccc acaccgcgac ggacaatgcg ccgatggaga tggtcgcgta
    13561 gatcagcgtg gtgtagccga agaccggctt gcgggagaac accgggaaga tctcggtgac
    13621 gatgccgaag aacggcaacg cgatgatgta cacctcgggg tgtccgaaga accagaacag
    13681 gtgctgccat agcagcacac cgccgttggc ggggtcgtag acgtgcgcgc cgaggtggcg
    13741 gtcggcggcc aggccgaaca gcgccgcggt gagcagcggg aaggccagca gcaccagaat
    13801 cgacgtcacc aggatgttcc aggtgaagat cggcatccgg aacatggtca tgcccggagc
    13861 gcgcatacac accaccgtgg tgatcatgtt gacgccaccg agaatggtgc cgagaccagc
    13921 gacggccaga cccatgatcc acagatcgcc accggctccc ggggagtgga tggcgtcggt
    13981 aagcggggtg taggcggtcc agccgaagtc ggccgcgccg ccgggggtga tgaagcccgc
    14041 gatcgcgatc atcgcgccga acaggaacag ccagaaggac aacgcgttga gccgcgggaa
    14101 cgccacgtcg ggagcgccga tctgcagcgg cagcaccagg ttggcgaacc cgaacacgat
    14161 cggcgtcgca tagaacagca acatcaccgt gccgtgcatg gtgaacagct ggttgtactg
    14221 c
//
LOCUS       DASYLX010000229         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3520166,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000229 DASYLX010000000
VERSION     DASYLX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520166"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGP27_05595"
     CDS             complement(<1..650)
                     /locus_tag="VGP27_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850822.1"
                     /translation="MTDAATPTGPITRGSVARVGAATVVTALCGYAVLYLAARDLEPA
                     GFSIFSVFWGAFGLVTGAANGLLQEATREVRSVRDAEVVSGPRTHPMRVAATVGVVAA
                     VVIAASSPLWSGHVFVESQWLSVALLSVGLAGFCLHATLLGMLAGVGRWTQYGALMVT
                     DAGMRVAVAAATFLIGWGLVGYLWATVAGAVAWLILLIASPSTRAAASLLTSGGTTT"
     gene            complement(647..>1603)
                     /locus_tag="VGP27_05600"
     CDS             complement(647..>1603)
                     /locus_tag="VGP27_05600"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV7850823.1"
                     /translation="VGFEELTEGVLVAGQPNGAASWFPCDDHPSSKASYHIQISTENP
                     YRAVANGELKSRRTRAAMTTWTYELAEPTSTYLITLQIGMYEGYRLQKSPVLMQAALP
                     ARLKRDFDYDFGRQPQMMKLFVKRFGPYPLSTGYTVVVTDDDLEIPLEAQGISIFGAN
                     HCDGTRGSERLIAHELAHQWFGNSVTARRWRDIWLHEGFACYAEWLWSDESGGETAEE
                     WARHYYARLRDSPQDLLLSDPGPRDMFDDRVYKRGALTLHALRGVIGDNNFFALLRDW
                     TKRHRHSTAVTDDFTGLAANYADVSLRPLWEAWLYSEDVPEL"
BASE COUNT          302 a          583 c          484 g          234 t
ORIGIN      
        1 gtcgtcgtgc caccggacgt cagcagcgac gcggccgcgc gcgtcgacgg cgaggcgatc
       61 agcaagatca gccatgccac cgctcctgcc accgtcgccc acaggtagcc gacgagcccc
      121 caaccgatca agaacgtggc cgccgcgacc gccacccgca tgccggcgtc ggtcaccatc
      181 agcgccccgt actgagtcca ccggcccaca ccggccagca ttcccagcag ggtggcgtgc
      241 agacagaacc cggccagtcc gacactcaac agtgcgacgc tgagccactg cgattcgacg
      301 aagacgtgcc ctgaccacag cggcgaactg gccgcgatca ccaccgccgc gaccacaccc
      361 accgtcgccg ccactcgcat cgggtgcgtg cgcgggcccg acaccacctc cgcgtcgcgg
      421 accgaacgga cttcgcgggt cgcctcctgc agcagaccgt tggccgcgcc ggtcaccaac
      481 ccgaacgcac cccagaacac actgaagatc gagaacccgg ccggctcgag gtctcgcgcc
      541 gccaggtaga gcaccgcata tccgcacagc gccgtcacca cggtggcggc accgactcgt
      601 gcgacgctgc cgcgagtgat cgggccggtg ggagtggccg cgtcggtcac aattccggca
      661 cgtcctcgga gtacagccac gcctcccaca gcgggcgcag cgatacgtcc gcgtagttgg
      721 ccgcgagacc ggtgaaatcg tcggtcaccg cggtggagtg acgatggcgc ttggtccaat
      781 cccgcagcag cgcaaagaag ttattgtcac cgatgacacc gcgcagcgca tgcagcgtca
      841 aagcgccgcg cttgtacacc cggtcatcga acatgtcgcg cgggcccgga tcggacagca
      901 acaggtcctg cggcgaatcg cggagccgcg cgtagtagtg gcgcgcccac tcttcggcgg
      961 tctcgccccc ggactcgtca gaccacagcc actcggcgta gcaggcgaaa ccctcgtgca
     1021 accagatgtc gcgccaccga cgggcggtca ccgagttgcc gaaccactgg tgcgccagct
     1081 catgggcgat caatcgctcc gatccccgag taccgtcgca atgattggca ccgaagatcg
     1141 aaataccctg cgcctcaagc ggtatctcga gatcgtcgtc ggtgacgacg acggtgtagc
     1201 cggtcgacag cgggtagggc ccgaaccgct tgacgaacag cttcatcatc tgaggctgcc
     1261 tgccgaagtc gtaatcgaag tcgcgcttga gccgggcggg cagcgcggcc tgcatcagca
     1321 ccggcgactt ctgcaaccgg taaccctcat acataccgat ctgtagcgtg atcagatacg
     1381 tcgacgtcgg ctccgccaac tcgtatgtcc acgtcgtcat cgcggcccgt gttcgccgag
     1441 acttcaactc cccgttggcg accgcccgat acgggttctc ggtgctgatc tggatgtggt
     1501 agctggcctt cgagctcgga tggtcgtcgc agggaaacca cgacgcggcc ccgttgggct
     1561 gccccgccac cagtacaccc tcggtcaatt cctcgaatcc cac
//
LOCUS       DASYLX010000230         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2094907,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000230 DASYLX010000000
VERSION     DASYLX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094907"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGP27_05605"
     CDS             complement(<1..633)
                     /locus_tag="VGP27_05605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177952.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7850824.1"
                     /translation="MVTVTYSPGPHLARFLATLSHATERPVTVLMADNGSSDGSPEEA
                     LDRFPNVRLLRTGENLGYGTAVNRAVAELRKDSEDLDLFIVANPDIQWGPGSIDALLE
                     AAGRWPRAASLGPLIRDPDGSVYPSARHQPSIIRGGMHAVIGPLWRSNPWTAAYRQDR
                     LEPSERPVGWLSGSCLLLRGAAFDEVGGFDERYFMYMEDVDLGDRFGKAGW"
     gene            complement(700..1563)
                     /gene="rfbD"
                     /locus_tag="VGP27_05610"
     CDS             complement(700..1563)
                     /gene="rfbD"
                     /locus_tag="VGP27_05610"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV7850825.1"
                     /translation="MANRIVISGAGGQVGRYLAVEAGGQGLDVVALTSAQWDITDAGA
                     AKRIVEPGDVVINCAAFTAVDAAESDPERAHAVNAVGPGHLARACANAGARLIHISTD
                     YVFDGSFDGGPRPYEIDDATGPLSVYAKTKLAGEQAVHEALPSAHVVRTAWVYTGVGS
                     DFVGVMRRLAAGDGPVTVVIDQIGSPTYVADLGLALLQIADRPSAPPLLHVANEGAAS
                     RFEQARAVFEGVGADPDRVQPASTEDVPRPAPRPPYTALSSSAATAAGLTPLRPWRDA
                     LAAALAEAVKN"
     gene            1631..3103
                     /locus_tag="VGP27_05615"
     CDS             1631..3103
                     /locus_tag="VGP27_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV7850826.1"
                     /translation="MPSRVIRIIAVMAAATIVIGTGVAWSKIRSFESGINHISSAALG
                     GGGEDGAIDILLVGLDSRTDAHGNPLSAEELATLRAGDDESTNTDTIILVRIPNNGKS
                     ATAISIPRDSYVDAPGIGKMKINGVYGSVHLEKLKQLVEQQGVDPSVAEPEATEAGRE
                     ALIKTVANLTGVTVDHYAEIGLLGFSLITDALGGVNVCLKDAVYEPLSGADFSAGWQK
                     LDGPQALSFVRQRHDLPRGDLDRVVRQQVVMASLAHEVISGKTLSSPATLNRLQEAVQ
                     RSVVLSDGWDVMQFAEQLQKLAAGNVAFATIPVLDENGWSDDGMQSVVRVDANQVHDW
                     VDGLLQDQDQGKTEELAYTPSKTAAEVVNATDINGLAASVSAVLAEKGFAQGPVGNHD
                     AGVLQTSQVFADSTDDLGAQAVSKDLGGLPVAADPSLPAGTVRVVLGNDYTGPGAGLD
                     GAGPTPAPVDPAASESTGGDAPPAPSPIITAGSENPDCVN"
     gene            3100..3816
                     /locus_tag="VGP27_05620"
     CDS             3100..3816
                     /locus_tag="VGP27_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HEV7850827.1"
                     /translation="MTNLSAALLDPLLGRDPVGPRVTYYDDASGERIELSTVTLANWA
                     AKTANLLRDELGAGPGSRIAVLLPAHWQTAAVLLGIWYIGAEVVLGPGEADVAMCTED
                     RLAEADAAVGPGEIAVLSLDPFGKPAADVPVGVTDYATSVRVHGDQIVLERQPGPALD
                     GRSVAELLAAATESAAAQGLTADDRVLSTATWKTPAELTDHLLAVFVAGASLVQVANP
                     DGAALQRRREVEKVTKSLMD"
     gene            3959..4210
                     /locus_tag="VGP27_05625"
     CDS             3959..4210
                     /locus_tag="VGP27_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850828.1"
                     /translation="MAGNRRPGKRVKVLAAVVGGSAVVALGTISAFTGGGSSGAPPVV
                     MSVGEMTMGDTATVGYSATIETSVAVPTDKAKPFGGSGG"
     gene            4245..>5099
                     /locus_tag="VGP27_05630"
     CDS             4245..>5099
                     /locus_tag="VGP27_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV7850829.1"
                     /translation="MINRRGFMVLSAAAGMTAACSSKTATKPTASPAPPPPVETKFAP
                     AEVDVDLGGPVVRTWAYTDRLPGNEIRIRKGQRLRAVVSNALPAETSIHWHGIAIVND
                     MDGVPPLTQAAIPTGGQFTYEFVVPDAGTYWYHSHVGTQLDRGLYGALIVEDPTEKAD
                     YDDELVVVLDDWIDGTATNPDQVLENLRKTGMKPMGPGGPGVSPTTPLGGDGGDVTYP
                     YFLINGRVPADPDMRDYRAGQRIRLRVINAGSDTAFRVAVPATLLNVIATDGYPVMPA
                     QSKSVIVGM"
BASE COUNT          854 a         1720 c         1738 g          787 t
ORIGIN      
        1 ccagccggcc ttgccgaacc gatctcccag gtcgacgtct tccatataca tgaagtagcg
       61 ctcgtcgaag ccgccgacct catcgaaggc ggcaccgcgc agcaacaggc aggatcccga
      121 cagccagccg accgggcgtt cgctgggctc gagccggtcc tggcggtagg ccgcggtcca
      181 cgggttggac cgccacaacg gcccgatcac cgcgtgcatg ccgccgcgga tgatgctggg
      241 ctgatgacgt gccgaggggt agaccgagcc gtccgggtca cggatcaacg ggccgagcga
      301 cgcggccctc ggccaccggc ccgccgcctc cagcagcgcg tcgatgctgc cgggacccca
      361 ttgaatgtcg gggttggcga cgatgaagag gtcgaggtct tcggaatcct tgcgaagctc
      421 ggcgacggca cggttcaccg cggtgccgta gcccaggttt tcgccggtgc gcaacagccg
      481 gacgttgggg aatcggtcga gggcttcctc gggactgccg tcgctggaac cgttgtcggc
      541 catgagcacc gtcaccggcc gctcggtggc gtgcgacagc gtcgcgagga accgagcgag
      601 gtgaggaccc ggtgagtacg tcaccgtcac cacatacagt tcgtcactca cgcgacgggt
      661 accacttcgt cactcacggc gtcgagggta gcggggaggt taatttttca ccgcttcggc
      721 caacgccgcg gccagcgcat cgcgccaggg ccgcagcggc gtcagtcccg ccgcggtcgc
      781 cgcgctgctc gacagtgccg tgtacggcgg ccggggtgcg ggtcggggca cgtcctcggt
      841 cgacgccggc tgcacccggt cggggtcggc gcccacgcct tcgaacaccg cccgcgcctg
      901 ctcgaaccgg ctggccgcgc cctcgttggc gacgtgcagc agcggcggtg ccgacggacg
      961 atcggcgatc tgcaacagcg ccaggccgag gtcggcgaca tacgtcggcg acccgatctg
     1021 gtcgatgacc acggtgaccg gtccgtcgcc cgccgccagc cgccgcatga caccgacgaa
     1081 gtcggatcca acgccggtgt aaacccacgc cgttcgaacc acgtgagcgc tcggaagcgc
     1141 ctcgtgcacg gcctgttcac cggcgagctt ggtcttggcg tagacgctga gcggcccggt
     1201 ggcgtcgtcg atctcgtagg ggcggggccc gccgtcgaac gagccgtcga acacgtagtc
     1261 ggtcgagatg tggatcagcc gggcgcccgc gttcgcgcat gcgcgggcga ggtgccccgg
     1321 cccgacggcg ttgacggcat gggcgcgctc tgggtcgctt tccgcggcgt ccacggcggt
     1381 gaaggcagcg cagttgatca ccacatcgcc gggctcgacg atccgtttcg cggcgccggc
     1441 gtcggtgata tcccattgcg ccgacgtcag cgccaccaca tcgaggccct gaccaccggc
     1501 ttcgaccgcc agatagcgcc cgacctgccc gcctgcaccg ctgatcacga tccgattcgc
     1561 cacccgccga gtctggcacg ccgggcagcg gctaccgggt atgcacccat cacgcaagta
     1621 acctgggcgg atgccatccc gcgtgatccg gatcatcgcc gtcatggccg cggcgacgat
     1681 cgtcatcggc acgggcgtgg cgtggagcaa gatccggtcc ttcgagtccg gcatcaacca
     1741 catctcgtcg gcggctctcg gcggcggcgg ggaggacggc gctatcgaca tcctgctggt
     1801 cggcctggac agccgcaccg acgcccacgg caacccgctg tcggccgaag aactggccac
     1861 gctgcgcgcc ggtgacgacg agtcgaccaa caccgacacg atcatcctgg tgcgcattcc
     1921 caacaacggg aagtccgcga ccgccatctc gatcccccgc gactcctacg tcgacgcgcc
     1981 gggcatcggg aagatgaaga tcaacggcgt ctacgggtcg gtgcacctgg agaagttgaa
     2041 gcagctggtc gaacagcagg gcgtcgaccc gtcggtggcc gagcccgaag ccaccgaagc
     2101 cggccgcgaa gcgctgatca aaacggtcgc caacctgacc ggtgtcacgg tcgatcacta
     2161 cgccgagatc ggcctgctcg gcttctcgct gatcaccgac gcactcggtg gcgtcaacgt
     2221 gtgcctgaaa gacgctgttt acgaaccgct ttcgggtgcc gacttttccg cgggctggca
     2281 gaagctggac gggcctcagg cattgagttt cgttcggcag cgtcacgacc tgccccgcgg
     2341 cgacctcgac cgggtggtgc gccagcaggt ggtgatggct tcccttgcgc acgaagtcat
     2401 ctcgggcaag acgctgtcga gccctgcaac gctgaaccgg ctgcaggagg cggtgcagcg
     2461 atcggtggtg ctgtccgacg gttgggacgt catgcaattc gccgaacagc tgcagaagct
     2521 ggcggcgggc aacgtcgcgt tcgccaccat cccggtgctc gacgagaacg gctggagcga
     2581 cgacgggatg cagagcgtcg tgcgagtgga tgccaaccag gtacacgact gggtcgacgg
     2641 ccttctgcag gaccaggacc aaggcaagac cgaggaactc gcgtacacac cgagcaagac
     2701 cgccgccgag gtcgtcaacg ccactgacat caacggcctg gccgcctcgg tgtcggccgt
     2761 cctggccgag aagggtttcg cacagggacc ggtcggcaac cacgacgcag gcgtgctgca
     2821 gaccagccag gtgttcgccg actccactga tgatcttggc gcacaagcag tttcgaagga
     2881 cctcggtggc ctgccggtcg ccgccgaccc gtctctgcct gcgggcaccg tccgggtggt
     2941 gctcggcaac gactacaccg gcccaggtgc gggtctcgac ggtgccggcc cgacgccggc
     3001 gcccgtcgac ccggccgcct ccgagtcgac gggcggggac gctccccctg cgccgtcgcc
     3061 gatcatcacg gcgggctcgg aaaatccaga ctgcgtgaat tgacgaatct gagtgccgca
     3121 ctgctggacc cgttgctcgg ccgggatccg gtcggccccc gcgtcaccta ttacgacgac
     3181 gcatccggcg agcggatcga gctgtcgacg gtcacgctcg ccaactgggc ggccaagacc
     3241 gccaacctgc ttcgcgacga actcggcgcg ggtccgggca gccggatcgc cgtgctgctg
     3301 cccgcgcact ggcagaccgc ggcggtcctg ctgggcatct ggtacatcgg ggccgaggtc
     3361 gtgctcgggc ccggcgaggc ggacgtcgcg atgtgcaccg aggatcggtt ggccgaagcc
     3421 gacgcggcgg ttgggccggg cgaaatcgcg gtgctgtcgt tggacccgtt cggcaaaccc
     3481 gcggccgacg tgccggtcgg ggtcaccgac tacgcgacgt ccgtgcgcgt gcacggcgac
     3541 cagatcgtcc tcgaacggca gcccggcccg gcgttggacg gccgttcggt agccgaactg
     3601 ctcgccgccg ccaccgaatc cgctgctgcg caggggttga ccgccgacga ccgcgtgctc
     3661 tcgaccgcaa cgtggaagac ccccgccgaa ctgaccgacc acctgcttgc ggtgttcgtc
     3721 gcaggagcat cgctggtgca ggtcgccaac cccgacgggg cagcgctgca acgccgccga
     3781 gaggtcgaga aggtaaccaa gagcctgatg gattgatggg ctaaccgctg tcagcgggag
     3841 ttgagtttcc tggaaatccg cttgggaggt cattcacaga ctgcttctag cgagtttcat
     3901 ggcttctcac gggtggtgat acggccacct tggagaagta agtgattggg gtagtgaaat
     3961 ggctggcaat cggcgacccg gcaagcgggt caaggtgtta gcggccgtag tgggcggcag
     4021 cgccgtggtt gcgttgggca cgatcagtgc ctttacgggc ggcggcagct caggcgctcc
     4081 tccggtggtg atgtcggtcg gcgagatgac gatgggtgac accgccactg tcggatactc
     4141 cgcgacaatc gagacgtcgg tggcggtgcc gaccgacaag gcgaagccat tcggaggctc
     4201 aggaggctag accccgcgcg caccgaagtg ccacgctggg cttcatgatc aaccggcggg
     4261 gcttcatggt gctgtctgct gccgcgggca tgacggcggc ctgctcatcg aagacggcga
     4321 ccaagcccac tgcgtcgccc gcgccgccgc cgcccgttga gacgaagttc gcacccgccg
     4381 aggtcgatgt cgacctcggc ggcccggtcg tccgaacctg ggcgtatacc gaccggctgc
     4441 cgggcaatga gatacggata cgcaagggcc agcggctgcg agccgtagtg tcgaatgcgc
     4501 tgcctgccga gaccagcatt cattggcacg gcatcgcgat cgtcaacgac atggatggcg
     4561 ttccgcccct gacgcaggca gcgatcccca ccggcggaca gttcacatac gagttcgtcg
     4621 tccccgacgc tggaacctac tggtatcact cgcatgtcgg gacgcagctg gaccgcggcc
     4681 tctacggcgc actgatcgtc gaggacccca ccgagaaggc cgattacgac gacgaacttg
     4741 tcgtggtcct cgacgattgg atcgacggga cagccaccaa ccccgatcag gtgttggaga
     4801 acctgcgtaa gactgggatg aaaccgatgg ggcccggcgg accgggagtg agcccgacga
     4861 ctccgctcgg cggcgacggc ggcgacgtca cctacccgta cttcctcatc aacggccgcg
     4921 ttcccgccga cccggacatg cgggattacc gtgccggaca acgcatccgg ttgcgtgtca
     4981 tcaacgccgg ctcggacacc gcgtttcggg tcgccgtccc ggccacgttg ttgaacgtca
     5041 tcgccaccga cggctatccg gtgatgccgg cgcagtcgaa atcagtcatc gtcggcatg
//
LOCUS       DASYLX010000231         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3849257,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000231 DASYLX010000000
VERSION     DASYLX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849257"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VGP27_05635"
     CDS             <1..1191
                     /locus_tag="VGP27_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7850830.1"
                     /translation="WLADIAPTVGEAKDAERNVRKWMRRRYRLLEMSQLPGRGWIEYE
                     PYGTVLIIGAWNFPIVLTLGPAVAAIAAGNTVVLKPSEVAPASSAVMAELVGKYLDND
                     AIAVIEGDGAVSQELIAQGFDRLLFTGGTEVGRKVYEGAAAHLTPVTLELGGKSPVIV
                     AADADIDVAAKRIAWTKLINSGQTCIAPDYVLVDAKVRDELVGKIKDAVTTFEADNPG
                     GKRIVNQRHFDRLTASLAATQGTIAVGGGSDASSISIQPTVVVDPDPNEQLMTDEIFG
                     PILPIVTVENLDGAIDFVNSRPKPLAAYLFTKAKSIRERVIKEVSAGGMVVNHLLFQF
                     ASNKLPFGGIGPSGMGAYHGKFGFEEFSHKKSVMTKPTRPDAMSFMYPPYTEKAKKLA
                     RKLI"
     gene            1235..>1893
                     /locus_tag="VGP27_05640"
     CDS             1235..>1893
                     /locus_tag="VGP27_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850831.1"
                     /translation="MPGVQDRVIVVTGAGGGLGREYALTLAREGASVVVNDLGGARDG
                     TGAGHNMADDVVAEIKDAGGRAVANYDSVAEPEGAEGIIKTALDEFGKVDGVVSNAGI
                     LRDGTFHKMEFANWDAVLKVHLYGGYNVIRAAWPHFREQSYGRVVVATSTSGLFGNFG
                     QANYGAAKLGLVGLINTLAQEGAKYNIKTNAVAPIAATRMTQDILPPEVFEKLTPEYV
                     AP"
BASE COUNT          366 a          611 c          638 g          278 t
ORIGIN      
        1 tggctggcgg acatcgcccc caccgtcggg gaggccaagg acgcggagcg caacgtccgc
       61 aagtggatgc ggcgccgcta ccgcttgctc gagatgtcac agctgccggg ccgcggctgg
      121 atcgagtacg agccgtacgg cacggttctc atcatcggcg cgtggaactt cccgatcgtg
      181 ttgacgctgg gacctgccgt cgccgccatc gcagcgggaa acaccgtcgt gctcaagccg
      241 tccgaggtgg cgccggcgtc atcggccgtg atggccgagc tggtgggcaa gtatctggac
      301 aacgacgcga tcgcggtcat cgagggcgac ggcgcggtga gccaggagtt gatcgcgcag
      361 ggcttcgacc gtctgctgtt caccggtggc accgaggtcg gccgcaaggt gtacgagggc
      421 gccgccgcgc acctgacgcc ggtgacactg gaactcgggg gcaagagccc cgtcatcgtc
      481 gccgcggacg ccgacatcga cgtcgccgcc aagcggatcg cctggaccaa gttgatcaac
      541 tccgggcaga cctgcatcgc cccggactac gtgctcgtcg atgcgaaggt ccgcgacgaa
      601 ctcgtcggca agatcaagga cgctgtcacg actttcgagg ccgacaatcc cggcgggaag
      661 cgcatcgtca accagcgtca cttcgaccga ctgacggcct cgctcgccgc gacgcagggc
      721 accatcgcgg tcgggggcgg ctcggacgca tcgtcgatct ccatccagcc gacggtggtg
      781 gtggatcccg atccgaacga gcaattgatg accgatgaga tcttcgggcc gatcctgccg
      841 atagtcaccg tcgaaaacct ggacggggca atcgacttcg tcaactcgcg gccaaagccg
      901 ctggcggcct accttttcac caaggccaag agcatccgcg aacgcgtgat caaggaggtc
      961 tcggcgggcg gcatggtggt gaaccatctg ctgttccagt tcgcgagcaa caagctgccg
     1021 ttcggcggca tcggtccgtc gggaatgggt gcctaccacg gcaagttcgg cttcgaggag
     1081 ttcagccaca agaagtcggt gatgaccaag cccacccgcc ccgacgcaat gagcttcatg
     1141 taccccccgt atacagagaa agcgaagaag ctggcgcgca agctgatctg acgtccgcgc
     1201 ccgcccgaca agaccgctaa gagagggaac accagtgcca ggagtgcagg atcgcgtcat
     1261 cgtcgtcacc ggagccggcg gcggactggg gcgcgagtac gcgctgacgc tcgccagaga
     1321 aggcgccagc gtcgtcgtca acgacctcgg tggtgctcgg gatgggacgg gcgcaggcca
     1381 caacatggcc gacgacgtgg tcgcagagat caaggacgcg ggcggtcgcg cggtggccaa
     1441 ctacgactcg gtggccgagc cggagggcgc cgagggcatc atcaagaccg cgctggacga
     1501 gttcggcaag gtcgacggtg tggtcagcaa cgcaggcatc ctgcgcgacg gcaccttcca
     1561 caagatggag ttcgcgaact gggacgccgt cctcaaggtt cacctgtacg gcggctacaa
     1621 cgtcatccgc gccgcatggc cgcacttccg cgagcagagc tacggacgcg tcgtcgtcgc
     1681 gacgtcgacc agcgggctgt tcggcaactt cgggcaggcc aactacggcg ccgccaagct
     1741 cggcctggtc ggcctgatca acacgctggc tcaagagggc gcgaagtaca acatcaagac
     1801 caacgcggtc gcaccgatcg cggccaccag gatgacgcag gacatcctgc cgccggaggt
     1861 tttcgagaag ctcacgccgg aatacgtggc gcc
//
LOCUS       DASYLX010000232        12038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1984935,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000232 DASYLX010000000
VERSION     DASYLX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984935"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VGP27_05645"
     CDS             <1..136
                     /locus_tag="VGP27_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEV7850832.1"
                     /translation="PVITGQTGDDPIFQGAADFDLELIESRTLDGNIQELIYRPALHV
                     "
     gene            complement(546..803)
                     /locus_tag="VGP27_05650"
     CDS             complement(546..803)
                     /locus_tag="VGP27_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850833.1"
                     /translation="MLLALAAVGVVVPTAARNEGPSYEAGYTAASNPKFVRSALSDRG
                     MSSASFCDELLRRTLTGARSTGLQQLDFLRGCEHAVHGVME"
     gene            complement(955..1395)
                     /locus_tag="VGP27_05655"
     CDS             complement(955..1395)
                     /locus_tag="VGP27_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850834.1"
                     /translation="MTDTPEPAAEGDSTIAAPSAQADPEATRQRRSPWTRLNRTNRIA
                     AVVLGVIAAVFVAVLIFGAGLLVGTEYGDSEGHHGAGSSEYGDGDRVSGEHEGDEGAS
                     AGDGDREGSEQADSESGQGARDEQAQPDAPHGQAPGESNGPPRP"
     gene            complement(1439..2074)
                     /locus_tag="VGP27_05660"
     CDS             complement(1439..2074)
                     /locus_tag="VGP27_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV7850835.1"
                     /translation="MNVALGTGILNPDTLLATFGLVGLLVTVFIETGLLVGFFLPGDS
                     LLFTAGVLAAQSPALVPLWVLLVAIPVAAILGDQCGYLIGRAAGPAVFDRPSAKRLGP
                     AQLARAQAFFENYGARTVLLARFVPLMRTLAPVMAGASGMNYRTFTTYNLLGGVAWGA
                     VVPLLGYLLGGVSFVRAHIELILIGVVVLSVLPLLVSYLKARCRRRTALAQ"
     gene            complement(2071..2883)
                     /locus_tag="VGP27_05665"
     CDS             complement(2071..2883)
                     /locus_tag="VGP27_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7850836.1"
                     /translation="MTTAIGPVMADGIAADSEAAGSILVLMVCAAVLGIGWRYRDRMT
                     RWWSTSVRVAGLLMGLVVVALQVSQHGWLVDVDNAATAWIVGHRNPVADHIAMAVTNA
                     FGPAETACAAVLVAIIAAVRFRSGVSGLTVIAAVGGASALCTALKLLLARARPPIGIQ
                     ETLETDYSFPSGHVTGTVALFGMLVVVVGMHRRDAVNACLRCTAVMVVAAVASSRLYL
                     GVHWLTDVVAGVLLGSAVVAIGATALRGLTEHDEGRPTSDHAVRPQRREVLL"
     gene            3001..3672
                     /locus_tag="VGP27_05670"
     CDS             3001..3672
                     /locus_tag="VGP27_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850837.1"
                     /translation="MRVLVVEDEVRLAETIRRGLVSEGFVVDVEHRGDDGFHTAASDN
                     FDAIILDIMLPGKNGYELVRDLRAKNVWTPVLMLSAKDGEYDLADAFDLGADDYLVKP
                     FSFVVLFARLRALVRRGAPERPAVLTAGEIELDPARHKVTRAGVELTLTPREYSVLEF
                     LMRHRDKVVSKSEIVRAVWDTNYDGDENIVEVYVRYLRRKIDLPFSGNSIETVRGVGY
                     RLVGG"
     gene            complement(3607..5040)
                     /locus_tag="VGP27_05675"
     CDS             complement(3607..5040)
                     /locus_tag="VGP27_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7850838.1"
                     /translation="MKPSASAIGSPSRWTVRTRSAVASTLVVAICLLLAGAALLLVLF
                     RTLEGSAQATAASRAAQLADQLSTEAPADLDRAMLATDSQVGVVQVTDQSGALVAQSA
                     GSPPEPVVDRRLPAGATQSIGRVQIGQDWDLWVTAAGVESPSGPMTVLVGADREPVED
                     VIMTVSTLLAIGGPIVIALVAFGTYRLVGAALQPVERIRARVSSMTHSKLAERIPVPA
                     ADDEVTRLAVTMNDMLDRLEASQSAQRRFISDASHELRSPLASITAALDLAHRRPDLL
                     DQSLIDDSLLPEAQRMYGLVEDLLLLARADERGGRNIKIDVDLDDIVLAEVERVRSLT
                     HLRVIADVKPARTSGDPGALSRLVRNLVDNAVRHAQEGVRLECAPVGGHARVVVSDDG
                     PGIPAADRQRVFGRFVRLDTPRDRAAGGAGLGLSIVAQIVEAHQGAVSIDDSASGGAR
                     FVVDLPLAADETVADTANGFDAVAGER"
     gene            5274..6527
                     /locus_tag="VGP27_05680"
     CDS             5274..6527
                     /locus_tag="VGP27_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850839.1"
                     /translation="MTETPRAAVVKPKRLMLSKVPEVTVWFWVIKILCTTVGESFADW
                     INMTLGVGLIPTSLIFTVVLVAVLAWQLRLDRYVPFVYWLTVVVVSVTGTLYTDILTD
                     VWGVPLALSTTVFAAILALVFGVWFARERTLSIHSITTTPRELFYWLAVLVTFALGTA
                     AGDWILELTGWGPGTSVLLPAGLIVAIVIGWRLGANAVLSFWLAYILTRPLGANLGDW
                     LGFPKDQQGLGLGVAVTSVIFLSAILATVVYLSITHADVIEKPHETTATNKPVREHVM
                     HGYYAVIALATGALLIWAGGQPHSTAAASENESGGSSVTATLAPGQSATAKFPAADVT
                     NFRTITQDTLVKVQAGDQAGAQARITDLETAWDDGQKSLEPLDETGWTVLDGQIDSAL
                     TAVRAGQPDPATERQALTTLLSSLR"
     gene            complement(6550..6756)
                     /locus_tag="VGP27_05685"
     CDS             complement(6550..6756)
                     /locus_tag="VGP27_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850840.1"
                     /translation="MRRLRRFGAFWYDFIVGDDWRVALTVALALATTFVANRLTGAAL
                     WWIVVAAVGAVLPLTIYRATRTQR"
     gene            complement(6759..7517)
                     /locus_tag="VGP27_05690"
     CDS             complement(6759..7517)
                     /locus_tag="VGP27_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850841.1"
                     /translation="MVNGSALFVTVFMACLVEAVEALTIILAAGTARDWRSAITGSLT
                     GVLVLVVAIAALGPAISAIPVDGLRLFVGGLLLIFGLQWLRKAILRASGFKALHDETL
                     AYQATLADAELAPKGRRVFVDDWYAFTLSFKGVVLEGVEVAFIALTFGANQGNIPLAA
                     LAAAAAVLLVAVVGFTVRAPLSRVPENSMKFVVGVMLTAFGIFWGAEGAGAHWPGDDI
                     ALLVLIPAIALYALALVAVFKRRAPVDVVVAQEG"
     gene            complement(7885..10596)
                     /locus_tag="VGP27_05695"
     CDS             complement(7885..10596)
                     /locus_tag="VGP27_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850842.1"
                     /translation="MVDARRPADAARRIAGLVIATWTLGLLRRRSGRMAATAIGIAAA
                     VALLSCLGIFLSAAQASMTARAARTVAVDWQVEVQPTTQLSFVLDEVRSTPHVQAALP
                     VGFGHSSGLSAIGGASTRTTGPAMVLGIPPEYRDRWPGEMRTLVGSDSGVLIAQQTAA
                     NLSAAPGDTVQIGRSGMPPVNVRIDGVVELPQADSLFQKVGAPIGAQPVAPPDNVLVL
                     PDVQWHAMFDPLAAGRPDLVSMQIHTALDRALPNDPSTAYKEVTGAAHNLEARGSGGA
                     LVGDNLGAALDAARGDAAYARILFLFLGLPAAALAVLLTATVVDSGSERRRREHALLR
                     ARGATSTRLLKLSAAEAIVVGVLGSAFGVAVAAVVGVATFGTSSFGANSSAALASAGA
                     SSAVGLAIAGMTVLAPAWREQRQTSVASARTWQRGISQPLWMRTGLDFVLLCAAEVIF
                     WQSGRNGYQVVLAPEGVPSISVSYWAFVAPALFWIGGALMIWRLSDLLLGRGRGLVAR
                     ALLPVTGQLARLLSNSLSRQRAPMARAIVVLALAAGFAVSTATFNATYQAQAEVDAKL
                     TNGADVTVTESPGANVPASTAKDLAAVPGVRGVEPIQHRFAYIGADLQDLYGVDPTRI
                     AAVTALQDRYFSGGSVDQLMSELSRQTDSILVSAETVTDFQLVAGDTINLRLPNAKTR
                     ELSTVRFRYVGVVNEFPTAPKDSFFVANASYVAQQTGNDAVGAFLIDTGGRDTTAVAQ
                     RVQVLVGPSAKVTDIAETRGTVGSSLTAVNLAGLTRIELGFGLCLAVAAGALVLALRF
                     AERRRTFAIANALGASRNQLRSFVVAEAWVLAFCGLVGGAIMGGLLSQVLVKALTGVF
                     DPPPGSLTVPWVYLFATGAATIITIGAVSLATARLASRSPAQALREL"
     gene            complement(10542..11216)
                     /locus_tag="VGP27_05700"
     CDS             complement(10542..11216)
                     /locus_tag="VGP27_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7850843.1"
                     /translation="MTTVAECTGVSRSFGSGRTAVLAVAGVDCQITSTARIAVTGSSG
                     SGKSTLLHLLAGVDTPTAGTVRWPKLPARPLGRPGGIGIVFQGPSLLPGFDVVENVAL
                     PMLLAGQDEDAADENARAALDLVGMSGAATKLPDELSGGQSQRVAIARVLVTRPALIL
                     ADEPTGQLDHANADRVITVLLSTATSIGAALVVSTHDPAVAARLDDRWSMRDGRLTPP
                     AESRAS"
     gene            complement(11213..11872)
                     /locus_tag="VGP27_05705"
     CDS             complement(11213..11872)
                     /locus_tag="VGP27_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7850844.1"
                     /translation="MNTSALEARSLYRFFRAGDEETLALQGVSLTVVRGEFVAVTGPS
                     GSGKSTLLSCLAGMDDPDGGDVTIAGSRVSHRAEPARSRIRARHIGMLFQSANLIEHL
                     TVSGNLALAQSFVRPPRRDTAELLRALDIHARVTAYPSELSGGELARAGLAVALANRP
                     TVLLADEPTGELDSVTEARILDLLTAAADRGTAVIVASHSPAVAAAASRVLRLDDGRM
                     C"
BASE COUNT         2266 a         3977 c         3811 g         1984 t
ORIGIN      
        1 ccccgtgatc accggtcaga ccggtgatga cccgatcttt cagggtgcgg ccgacttcga
       61 cctcgagctg atcgagagcc gaacgctcga cggcaatatc caagagctca tctaccggcc
      121 cgccctgcat gtctgattcc atccatgcac aacaccccgt caagcagcca aacctgagcg
      181 gaagttccag gtgacgaccg atcgggccga cctcgtcgcc ctcgtccgct ccgattgatc
      241 tggtcattcg acgagcttgc cggcgggcgt tgaccagctt ccagctgcgt tgctttacga
      301 gcgcaccggt gatcacagcc atggacggtc attgaggacc gcccggcttg ctcggccgtc
      361 tggacggatg tcgatgctgt agccgaccca ccaacgcttg tgcttcgcga tcgtcaacat
      421 cgacctcacc caccttcgca ggccggctgt gggactgatt gtgccacaat cactctcgcg
      481 cccttgcatc aattgctttc cagatatcgg cactatcagc agacctcagt ggtgggcctc
      541 gccacttatt ccatgacgcc gtgaaccgcg tgctcacaac cgcgaaggaa atcgagttgc
      601 tgtaagccgg tcgatcgcgc gccggtgagt gtgcgccgga gcagctcatc gcagaacgag
      661 gccgagctca tccctctgtc agacaacgcc gaacgcacga acttcggatt cgacgccgct
      721 gtatagcccg cctcgtatga tgggccttcg ttgcgggcag cagtgggtac aaccacgccg
      781 accgcggcga gcgccagcag cacgacagac gaccgggctg ccagttgcat cgcgtgttgg
      841 tgcatgaggc gcgcctcctc gtcgactcca tcgggtcgcg gtacttccat ggtggcactg
      901 atcgcggtac caagccgcaa gccttcggac acttagcgat tcagcgtcgg cccctcaggg
      961 tcgcggcgga ccattcgact caccaggtgc ctgaccgtgt ggtgcgtcgg gttgagcttg
     1021 ttcgtcgcgc gcgccttgac cgctttcgct gtcggcttgt tcgctgccct cgcgatcacc
     1081 gtcgcctgcg gatgcgcctt cgtcaccttc gtgttcgccc gacacgcggt cgccgtcgcc
     1141 gtactcagat gatccggcgc cgtggtggcc ctctgagtcg ccatactccg tcccgaccag
     1201 caggccggcg ccgaagatca gcacggcgac aaacactgca gctattacgc caaggaccac
     1261 tgcggcaatt cgattggttc gattcagccg cgtccacggg gatcgccgct ggcgggtagc
     1321 ttcggggtca gcttgggcgc tgggcgccgc aatcgtcgag tcaccctcag cggcaggttc
     1381 tggcgtgtca gtcatgacat caacgtgcca gtgcccacct gaaaaaacgc tgaactgact
     1441 actgagcgag cgctgttcgg cgccgacagc gcgccttcag ataactgacg agcaagggca
     1501 gcaccgacag cacgacgacc ccgatgagta tcagttcgat atgcgcacgg acgaacgaaa
     1561 ccccgcccag caagtagccg agtaacggca cgacggcgcc ccacgccacg ccgccgagga
     1621 ggttgtacgt cgtgaaggtt cggtagttca tgccggatgc acccgccatg accggcgcca
     1681 acgttcgcat gaggggtacg aatcgggcga gcagcacagt gcgggctccg taattctcga
     1741 agaacgcctg cgctctggcc aactgcgccg gccccagccg tttggcgctg gggcggtcga
     1801 acaccgcggg cccggcggcc cgtccgatca ggtagccaca ttgatcgccg agtatcgcag
     1861 cgacgggaat cgcgacgagc aggacccaca atggaaccaa tgccggggac tgcgccgcaa
     1921 gcactccggc ggtgaacaac agggagtcgc ccggcaggaa gaaccccaca agcagtcccg
     1981 tctcaatgaa gacggtgaca agtagtccta ccagcccgaa ggtagccagg agggtgtccg
     2041 ggttgagaat gccggtgccc agcgcgacgt tcacaatagg acttcgcgcc gctgcgggcg
     2101 gaccgcgtga tcggaggttg gccgcccttc atcgtgttcg gtgagcccgc gcagcgccgt
     2161 cgcgccgatc gctactaccg ccgacccgag caacacaccg gccaccacat cggtgagcca
     2221 atgaaccccg agataaagcc gactcgacgc gaccgcagcg acgaccatga ccgcggtgca
     2281 cctaaggcat gcgttgacgg catctctccg gtgcattcct acgacaacca ccagcatgcc
     2341 gaacaacgcc acggtgccgg tgacgtgacc cgagggaaac gagtagtcgg tctccagcgt
     2401 ttcctgaatt ccgatgggag gtcgagcgcg cgccagcagc agtttcagtg ccgtgcacaa
     2461 cgcagaggcg ccgccgacag cggcgataac ggtgagcccg ctgacgccgg agcggaagcg
     2521 aacagcggca attatcgcga cgagaactgc tgcacaagca gtttcggccg gcccgaaggc
     2581 attggtcacc gccatggcta tgtggtcggc gaccgggttg cgatggccga cgatccacgc
     2641 ggtggcggcg ttgtcgacgt ctaccagcca accgtgttgg ctcacctgca gcgccacgac
     2701 caccaggccc atcagcaagc cggcaacacg aaccgacgtc gaccaccacc gcgtcatgcg
     2761 gtcgcggtag cgccaaccga tccccagtac ggcagcacag accatcagaa ccagaatcga
     2821 ccccgctgcc tcgctgtccg ccgcaatccc atccgccatc acgggcccga tggcggtcgt
     2881 catttcgccc accggcacag agtcgccgtt ggcggctgaa cggacgctga gaagtcttgg
     2941 ccgcaaatac agttgttctg gtgtacttag tcgagtcact acgatcggat ggatgtaacc
     3001 gtgcgggtac tggtagtcga ggacgaggtc cgcctcgccg agacgattcg ccgcggactg
     3061 gtgtccgaag gtttcgtcgt cgacgtagag catcgtggcg atgacggctt tcacaccgcc
     3121 gcgtcggaca atttcgacgc gatcatcctt gacatcatgt tgccgggaaa gaacggatat
     3181 gaactcgtcc gggacctgcg cgccaagaac gtgtggacgc ccgtgttgat gctgtccgcc
     3241 aaggacggcg agtatgacct cgccgacgcc ttcgatctgg gtgcggacga ctacttggtc
     3301 aaaccgttct cattcgtcgt gctgttcgcc aggcttcgag cactggtgcg gcgcggcgca
     3361 cctgagcgtc ccgccgtgtt gactgccggc gagatcgagc tcgatccagc gcgacacaag
     3421 gtgacccggg caggagtcga gctgaccttg acgccaaggg aatacagcgt gttggagttc
     3481 ttgatgcggc accgtgacaa agtggtcagc aagagcgaaa tcgtcagagc ggtgtgggac
     3541 accaattacg atggcgacga gaacatcgtc gaggtgtacg tcagatacct gcggcgcaag
     3601 atcgacctac cgttctccgg caacagcatc gaaaccgttc gcggtgtcgg ctaccgtctc
     3661 gtcggcggct aggggcaggt cgacgacgaa acgcgctccc ccactggcgg aatcgtcgat
     3721 gctgaccgca ccctggtggg cctcgacaat ctgcgcgacg atcgacaagc ccaggccggc
     3781 accgccggct gctcggtcgc gcggcgtatc caaccggacg aagcggccga acacgcgttg
     3841 gcgatcggcc gccggtatcc cgggcccgtc gtccgagacg acgacccggg cgtggccgcc
     3901 aacgggcgcg cattcgagtc gaacgccttc ctgcgcgtgc cggacggcgt tgtcgaccag
     3961 attgcggacc agacgcgaca aggcgcccgg atcgccagaa gtccttgccg gcttcacgtc
     4021 ggcgatgacc ctcaggtggg taagcgaacg aacgcgctcg acctcggcca agacgatgtc
     4081 gtcgagatcg acgtcgatct tgatgttgcg gccgccgcgc tcatccgcgc gggcgagcag
     4141 caaaagatcc tccaccaggc cgtacattcg ctgcgcctcg ggaagaagcg aatcgtcgat
     4201 caatgactga tccaagagat ccggccgtcg gtgggcaaga tccagggcgg cagtgatcga
     4261 tgccaacggg ctgcgcaatt cgtgtgacgc gtcgctgatg aagcggcgtt gtgcgctctg
     4321 actcgcttcg agtcgatcca gcatgtcgtt catggtcacg gccaaccggg tgacctcgtc
     4381 atccgccgca ggtaccggta tccgttcggc aagcttggag tgggtcatcg acgaaacgcg
     4441 tgcgcggatg cgttcgacgg gctgcaacgc cgcgccgacg agtctgtaag tgccgaatgc
     4501 gaccagcgcg atcacaatcg gcccaccgat ggcaaggaga gttgagacgg tcatgatgac
     4561 gtcttcgacc ggctcgcgat ccgcgccgac gaggacagtc atgggtccgg atggactctc
     4621 gacgcctgcc gccgttaccc acaggtccca gtcctggccg atctgcactc ttccgatcga
     4681 ctgcgtcgcg ccggcgggca gtctgcgatc caccacgggt tcaggcggac tgcctgccga
     4741 ctgcgcgacg agcgctccgg actgatcggt gacctgaacc acaccgacct ggctgtcggt
     4801 cgccagcatc gctctgtcga ggtcggcggg cgcttccgtg ctcagctggt ctgccagctg
     4861 ggcggcgcga gaagcggccg tggcctgcgc ggacccctcc agtgtccgga acaggacgag
     4921 caacagcgcg gcgcctgcca ataacaaaca gatcgccacc accagtgtcg acgcgaccgc
     4981 cgaacgggta cgcaccgtcc accgcgacgg cgatccgatg gccgaggccg aaggcttcat
     5041 gccttcagtg tgactcccgt cggccggcgg atggctcacc cggtccactg cgctcgttcc
     5101 cttcactacc gacgccaacc ctgatctgcc ttgcactgac cacggcgtgg acgactcaga
     5161 gtgagccaca gtcgctgaag agcggctgag gaccgctaag aattgggcgc cgacaagcgg
     5221 ggttgcaatc gtcctcagcg gggcttcagc tacgtccccc tacggtctgc gtcatgaccg
     5281 agacacccag agctgcagtc gtcaaaccca agcgcttgat gctgagcaag gtgcccgaag
     5341 taaccgtgtg gttctgggtc atcaagatcc tgtgcacgac cgtgggtgag agcttcgccg
     5401 actggatcaa catgacgctg ggcgtcgggc tgattccgac gtcgctcatt ttcacggtcg
     5461 tgctcgtcgc ggttttggcg tggcagttac gcctcgaccg ctacgtccca ttcgtctact
     5521 ggctcacggt cgtcgtggtc agcgttaccg ggacgctcta tacggacata ctcaccgacg
     5581 tttggggcgt tccactcgca ctgagcacaa ccgtgttcgc cgcgatactg gcgctggtct
     5641 tcggtgtgtg gttcgcgaga gagcgcacgc tttcgatcca cagcattacg acgacaccac
     5701 gagagctgtt ctactggctt gcggttctgg tcacgttcgc cctcggtacc gcggccggcg
     5761 actggatcct cgagctcacc ggatgggggc ccggtacgtc ggtgctgctg ccggccggac
     5821 tcatcgtggc gatcgtcatt ggttggcggc tcggcgccaa cgccgtcctg tccttctggt
     5881 tggcctacat cctgacccgc ccactgggcg ccaacctcgg cgactggctc gggttcccga
     5941 aggatcaaca gggccttggt ttgggcgtcg ctgtcacgag cgtcatcttc ctcagtgcga
     6001 tcctggccac ggtggtgtac ctctcgatta cccacgccga cgtcatcgag aagccgcacg
     6061 aaaccaccgc caccaacaaa ccggtccgcg agcatgtcat gcacgggtac tacgccgtca
     6121 tcgcattggc cacaggggct ctgctcatct gggccggcgg ccaaccgcac agcactgcgg
     6181 ccgcaagcga aaacgagtcg ggcggctcgt cagtgaccgc gacgctggct ccgggtcagt
     6241 cggcaacggc gaagttccca gctgcggacg tcacgaattt ccgcactatc acgcaggaca
     6301 cgcttgtgaa ggtgcaagcc ggcgaccagg cgggtgcgca ggctcgcatc acggatctcg
     6361 aaaccgcttg ggacgacggg caaaagagcc tggagccctt ggacgagaca ggctggacgg
     6421 tactcgacgg ccagatcgac agcgcgctga cggctgtgcg cgccggccag cccgaccctg
     6481 ccacagagcg gcaggccctc accacgctgc tgtcctcgct ccggtagaac ggttgcccgg
     6541 caacgctgct caccgctggg tacgcgtcgc ccggtagatc gtcaggggca acaccgcgcc
     6601 gaccgcggcc accacgatcc accacagggc tgcgccggtc aggcggttcg ccacgaaggt
     6661 ggtggcgagt gcgagcgcaa cggtcagcgc aacacgccag tcgtcaccga cgatgaagtc
     6721 gtaccagaag gcgccgaacc ggcgaagtct gcgcacggtc aaccttcctg agccacaacg
     6781 acgtcaacag gtgcgcggcg cttgaacacg gccaccagcg ccagtgcgta cagcgcgata
     6841 gccgggatca gcaccagcag ggcgatgtcg tcgccgggcc agtgtgcgcc agcgccttct
     6901 gcgccccaga agatcccgaa cgccgtgagc atcaccccga caacaaactt catgctgttc
     6961 tccggcactc gcgacagcgg cgcccgaacg gtgaagccga caaccgcgac aagtagcacc
     7021 gctgccgccg cggccagtgc cgccaacggg atgttgcctt ggttggcgcc gaatgtgagg
     7081 gcgatgaagg cgacctccac accctccagc acgactccct tgaacgacag cgtgaacgcg
     7141 taccagtcgt ccacgaacac ccggcggccc ttcggggcga gttccgcatc ggcgagcgtg
     7201 gcctggtagg ccagggtctc gtcgtgaaga gccttgaagc cgctggcccg caagattgcc
     7261 ttgcgcagcc attgaagtcc gaagatcaac agcagcccac cgacgaagag ccgcaagcca
     7321 tccacgggga tcgccgagat ggcgggcccc agcgctgcga tcgccaccac cagaaccagc
     7381 acacctgtca gcgagccagt gatcgccgaa cgccagtcac gtgcagtgcc cgcagcaaga
     7441 atgatcgtca gcgcctcgac ggcctcgacc agacacgcca tgaagaccgt gacgaagagt
     7501 gccgatccat tcaccatcta cctcgccgac atcatagggt gccgaaggca cggcccgact
     7561 aatgaattgt aacggcggtt aggacatgac ggccgagtga acgcctgcgg cacagcgtcc
     7621 tttaaggatg tcgatgacag cttgtcgcaa tgacagagac atcggctgac gctgagcagg
     7681 cggcctactt ccgcgaatca ctggacgcgg agcgacgaca attgctggaa gggatcctcg
     7741 agagaaggcc cctcatcgag ggtgatgcca gccggctaca ctcggccgtc gatcgcgcag
     7801 agtcccaagt gcgacaccta gaccacctga tcgagcggct cgacgctcgc ttcccgagcc
     7861 actggcctga ccgtgactga agtctcacaa ctcgcgcaag gcctgcgcgg gtgagcggct
     7921 tgccaaccgc gccgtcgcca gggacaccgc gccgatcgtg atgatcgttg ctgcccccgt
     7981 cgcgaaaagg tagacccatg gaaccgtgag tgaccccggc ggcgggtcga atacgccggt
     8041 gagagccttt accagcacct gtgacagcag cccacccatg attgcgccgc caacgagacc
     8101 gcagaaggcc agcacccacg cctccgccac gacgaaggac ctgagttgat ttcgcgaggc
     8161 gccaagcgca ttggcgatcg cgaaggtgcg gcgacgttcg gcgaaacgca acgcgagcac
     8221 aagtgccccg gccgcaacgg ccaggcacag cccgaacccg agctcgatgc gggtgagccc
     8281 ggccaggttg acggcggtca ggctggaccc gacggtgcca cgcgtttctg cgatgtcggt
     8341 caccttcgcc gacggtccca cgagcacttg aacccgttga gccaccgctg tggtgtcccg
     8401 gccgcccgtg tcgatcagga atgcgccaac ggcgtcgtta cccgtctgct gggcgacata
     8461 gctcgcattc gcgacgaaaa agctgtcttt cggggcggtc ggaaactcgt tgacgacgcc
     8521 gacatagcga aatcgcacgg tgctgagttc gcgggtcttg gcattgggca gccgcagatt
     8581 gatggtgtct ccggcgacca gctggaaatc tgtcacggtc tccgcgctta ccaggatcga
     8641 atcggtttgt ctggacagct cactcatcag ctggtcgacg ctgccgccgc tgaagtaccg
     8701 gtcctgcaat gcggtgacgg ctgcgattct ggtcgggtcg acaccgtaca ggtcctggag
     8761 gtcggcgccg atgtaggcga accggtgctg gatcggttcg acgccgcgca ctcccggtac
     8821 cgcggcgaga tccttggccg tggatgcggg cacattcgcc cccggcgact cggtgaccgt
     8881 cacgtcggca ccgttcgtca gtttggcgtc gacctcggcc tgggcttgat aggtggcatt
     8941 gaacgtcgcc gtcgacacgg cgaatcctgc ggccaatgcg agcacgacga tcgccctggc
     9001 catgggcgca cgctgcctcg agagcgagtt cgacagcaga cgcgcgagct gacctgtcac
     9061 gggcaacagg gcgcgggcca ccagcccgcg accacgaccg agcaagaggt cactcagcct
     9121 ccagatcatc agtgcgccgc cgatccagaa caacgcgggc gcgacgaacg cccagtagga
     9181 gaccgagatc gacgggacgc cttcgggagc gagaacgacc tgatatccgt tgcgcccgga
     9241 ttgccagaag atcacttccg cggcacagag caacacaaag tccaaaccag ttctcatcca
     9301 gagtggctga ctgatcccgc gctgccaggt gcgcgctgac gcgacggacg tttgacgctg
     9361 ctcgcgccac gccggcgcca gcacggtcat ccctgcgatc gccagtccaa ctgcggagga
     9421 tgcacctgcc gacgccagcg cggccgagga gttcgcgccg aagctcgaag tcccgaaggt
     9481 ggcgactccg acgacggctg cgaccgcaac gccaaaggca ctgcccaaaa caccgaccac
     9541 aatcgcctcg gccgccgaca atttcagcag tcgcgtgctg gtggcgcctc gggcgcgcag
     9601 cagtgcatgc tcccgtcgcc gtctctccga tcccgagtcg accacggttg cggtcagcag
     9661 taccgcgagg gccgcggcgg gtaacccaag gaacaggaac aggattcgcg catatgccgc
     9721 gtcaccgcgg gccgcgtcga gggcggcgcc gagattgtca ccgactagcg cgccgcccga
     9781 gcccctggcc tccagattgt gcgccgcgcc cgtaacctct ttgtatgccg tcgacggatc
     9841 attcggcagt gcgcgatcga gcgcggtgtg gatctgcata ctgaccagat cgggccggcc
     9901 ggcggcaagc gggtcgaaca ttgcatgcca ttggacgtca ggaagcacca ggacgttgtc
     9961 cggtggcgct accggttggg ctccaattgg agcgccgact ttctgaaaca gtgaatctgc
    10021 ttgtggcagt tcgactaccc cgtcgatgcg aacgttcacc ggaggcattc ccgaccgccc
    10081 gatctggacg gtgtcacccg gcgcggcact cagattggcg gccgtctgtt gggcgatgag
    10141 aacaccgctg tcggagccga ccagggtgcg catctccccc ggccaccggt cccggtattc
    10201 gggaggaatt cccagcacca tcgcgggtcc ggtagtccgg gtactggcac cgccgattgc
    10261 actcaatccg gaggaatggc cgaacccgac cggtaacgcg gcttgaacgt ggggcgtgga
    10321 ccgaacttcg tccaagacga aggacaattg tgtggttggc tggacttcga cctgccagtc
    10381 gaccgcgacg gtgcgggcgg cgcgagcggt catggatgcc tgtgcggccg acaggaatat
    10441 gccaagacag gacagcagcg cgacggcggc ggcgatgccg atcgcggtcg ctgccatccg
    10501 tccgctgcgg cgccgtagca gccccagcgt ccaggtcgcg atcacgaggc ccgcgattcg
    10561 gcgggcggcg tcagccggcc gtctcgcatc gaccatcggt catcgagcct ggcagcgacg
    10621 gcgggatcgt gggtggagac cacgagggcc gctccgatcg acgtcgcagt gctcagaagc
    10681 acggtgatga cccggtcggc gttggcatgg tcgagttgtc cggtcggctc atcggcaagt
    10741 atcagggcgg ggcgcgtgac aagcacccgc gcgatcgcga cgcgctggga ttgaccacct
    10801 gacagttcat cgggcaattt cgtcgcagct ccgctcatgc cgacgagatc cagtgcggcc
    10861 cgggcgttct catctgcagc gtcctcgtcc tgtcccgcca agagcatggg aagtgcgacg
    10921 ttctcgacga cgtcgaaccc aggcaaaagg ctcgggccct ggaagacgat gccgatccct
    10981 cctggtcgcc cgagcggccg cgcgggcagc ttgggccacc gaacggttcc ggcagtaggc
    11041 gtatcgacgc ctgcgagcag gtgcaagagg gtggactttc cagatcccga ggatccggtc
    11101 actgcgattc gggctgtcga cgtgatctga caatccactc cggcgacagc gagcaccgcc
    11161 gtccgacccg agccgaagct acggctcaca ccggtgcact cggcgacagt cgtcaacaca
    11221 ttcgtccgtc gtctaggcgt aggactcgtg aagcggcagc cgccacagcg ggactgtgac
    11281 tcgcgacgat caccgccgtc ccgcggtcgg ctgcggctgt cagcaagtcg aggattcgtg
    11341 cctccgtgac gctgtcgagc tcgcctgtcg gctcgtccgc caggagcacc gtcggccggt
    11401 tcgcgagcgc aaccgcgagg cctgctctgg caagttctcc tccggagagc tcgctcggat
    11461 aagcagtcac tcgggcgtgg atgtccaagg ctctcaacag ttccgccgtg tcccggcggg
    11521 gcgggcggac aaatgattgc gcgagggcga gattcccgga aacagtgagg tgttcgatca
    11581 ggttcgcgct ctggaacagc atcccgatgt gtctggcccg aattcgcgac cgcgcgggtt
    11641 cggcacggtg actgacccgc gagccggcga tggtgacgtc accgccgtcg ggatcatcca
    11701 ttcccgccag acacgacaat agggtcgact tgcccgaacc ggacggtccg gtgactgcca
    11761 cgaactcacc ccgtaccacc gtcaacgaca cgccctgaag cgcaagcgtt tcctcgtccc
    11821 cggcacggaa gaaccggtaa agagaccgag cctccaaggc actggtgttc attcgtcggt
    11881 tatcagcgcc actgcaacga gtcggtgatc gtccgccacg gatcgacgtt gtccgctccc
    11941 acgggtccag agagggtcag gatcacctcc tggcctgcgc gccagaatga gtaacgttct
    12001 acggcgtcga ttccggcctt gccggtcacc gggttcgg
//
LOCUS       DASYLX010000233         6841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3849307,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000233 DASYLX010000000
VERSION     DASYLX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3849307"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VGP27_05710"
     CDS             complement(<1..960)
                     /locus_tag="VGP27_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7850845.1"
                     /translation="MVDVHVARSCDARAGSRLTGARLAGLLVRPTAPPLAVGIVVAAC
                     FIAAETLLVYLLEKIFPGMPFGVVFLLGVLVVSAGWGFGLAVSTTLVSALVYAFVHLD
                     VDGSIFPITTQDSVAIIIFVPIALLANVLVGQARLRAAEANRRREEAEESRDELRVLA
                     DQQAALRRIATLVARAVAPYEVFSAVTEELARCLGVTHATLVRYAADGSAVLLASHDD
                     RGPQKKIPIGKRFSFDGESVPAMVFRTGRAARRDSHEDSPTPDIQYLHELGLRSGVGV
                     PIIVGGRLWGAANVGSSRHEPLPPNTEARVGDFADLVATAIANA"
     gene            complement(971..1879)
                     /locus_tag="VGP27_05715"
     CDS             complement(971..1879)
                     /locus_tag="VGP27_05715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850846.1"
                     /translation="MTRPAVTVLGTGTMGAPMARNLLRAGLDVRVWNRTLAKAAVVGV
                     DARRMSTPGDAAAGADVLITMLADGAAVEQVMTGPTGALSMLPAGAIWIQMGTVGAEW
                     TDRFAGLVARHDVAFVDAPVSGSSGAAEEGELVILASGASAVRSRVEPIFDVLGHQTL
                     WLQHAGDGSRLKLALNNWLAVLVEGMVETLTLSEALGLDPQLLLEAVGDGPMGSHYAL
                     AKGAAMLNGDFVPGFPLRHATKDAELALTAAHRHGVELPLTSALLPRWYEAIAGDHGD
                     DDVAAAVTAAATTASPPGDHLTVFAT"
     gene            complement(1876..2853)
                     /locus_tag="VGP27_05720"
     CDS             complement(1876..2853)
                     /locus_tag="VGP27_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7850847.1"
                     /translation="MKTTTLGRSGLEVSRIAFGTWQLGGDWGQFDEATAVAAIRGARE
                     LGVNFFDTAQAYGFGASERILGRALRHQLIGNRNELVIATKGGLRQIDSGIVRDASPE
                     WLRKGVDASLTALGIDHIDLYQVHWPDATVPVAETAGALRDLVTEGKIGHVGVSNYDS
                     EQMAEFSATLPVETLQPPYHLFRREIEDQVLPYCRAHDIGVLAYGPLAHGLLTGTLSV
                     HTAFDEDDWRATSGVFSGGTFRRNLATVRTLEKFAADRGISVSQLAIAWTLANPAVHV
                     AIVGARHVDHIEDSLRAVDISLTDSDLDEIETIMDDATPVAGPSPEGMP"
     gene            3091..3477
                     /locus_tag="VGP27_05725"
     CDS             3091..3477
                     /locus_tag="VGP27_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850848.1"
                     /translation="MRCLIVDDSAGFIDAARGLLECQGLSVVGVASTSGEALRLFEEI
                     RPDVTLVDINLGRESGFELAEQLHQVGGSTPSPVILISTHAAQDFADMIETSPAVGFL
                     SKSALTGAAIRDLVDGLASVEEGDHR"
     gene            complement(3445..4098)
                     /locus_tag="VGP27_05730"
     CDS             complement(3445..4098)
                     /locus_tag="VGP27_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850849.1"
                     /translation="MIAMQVVVADDDLLLREGLASLLDRSGFDVVGQAGDGGQLLALV
                     RERTPELVVADIRMPPTHSTEGLDAARVIREEFPDIAILLLSAHVEVEHAMELLGSGH
                     SIGYLLKSRVTDVADFLETLERIAKGASVVDPALVSELVSARRRDDPLAALSAREREV
                     LALMAEGRSNAGIAHRLWVTEGTVEKHVRSILTKLNLPEAGEDHRRVLAVITFLDAC"
     gene            complement(4210..5802)
                     /locus_tag="VGP27_05735"
     CDS             complement(4210..5802)
                     /locus_tag="VGP27_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD7 family fatty acid--CoA ligase"
                     /protein_id="HEV7850850.1"
                     /translation="MAALTERVVDGTSMYVAPAITGLVDLFDRQVIDRPEGQALVLTK
                     DRIPLSYGALDALVNDMAGRLRSKGLRRGDAVGLISANNVEFVVALLGAARAGLVIAP
                     LDPALPQSEMAARLERLGAQGILVGPRAVDSAPPAQFPIATCEVRVDVAGGGITTATL
                     EIGASLGRRVRGPVDDLSDDDAIVLFTSGTTDQAKMVPLTNANLAASVRAICGSYELG
                     PDDATVAVMPLFHGHGLLAVLLSTLASGGCVLLPEHGRFSARTFWDDMRAAPATWFTA
                     VPTIHQILLQRSPREYPGQQEVPLRFVRSCSAPLDTVTARAMERTFGAPVLSAYGMTE
                     TSHQATSLPLPLRGPVKHGSVGRATGVDLRVVDNDGHICPAGIEGEVWVHGPTVARGY
                     LANQAGTAHSFAEGWFRTGDLGSVDEDGYLFLTGRIKNIINRGGEKISPEHVEAVLAG
                     CRGVTEAAVFAMPDATYGERVGAVVVLSQNESIGPETILRECKSKLSPFEVPERLEVV
                     AALPHTAKGALDRRAVEEQFAH"
     gene            complement(5906..6841)
                     /locus_tag="VGP27_05740"
                     /pseudo
     CDS             complement(5906..6841)
                     /locus_tag="VGP27_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
BASE COUNT         1236 a         2290 c         2169 g         1146 t
ORIGIN      
        1 ggcgttggca atcgcggtcg ccaccaggtc cgcgaagtcc ccgacgcgcg cctcggtatt
       61 cgggggtagc ggctcatgtc gcgacgatcc gacattggcc gcgccccaca gccggccgcc
      121 cacaatgatc ggcacgccga cgcctgagcg cagacccagc tcatgtaggt attgaatgtc
      181 cggagtgggc gaatcctcgt ggctgtcccg ccgggcggcc cggcccgtgc ggaacaccat
      241 cgccgggacg ctttcgccgt cgaacgaaaa ccgtttaccg atcggtatct ttttctgcgg
      301 cccccggtcg tcgtggctgg cgagcagaac cgcagaaccg tcggccgcat agcggaccag
      361 agttgcgtgg gtgactccga ggcaccgggc caactcctct gtcaccgctg agaacacctc
      421 gtacggcgcc accgcccgcg ccaccagggt ggcaatgcgt cgcaaggcgg cctgctggtc
      481 agcgagcacg cggagctcat cccggctctc ctcggcctcc tcgcggcgcc gattcgcctc
      541 ggcggctcgt aatcgcgcct ggccgacaag cacattggcc agtagcgcaa tgggcacgaa
      601 gatgatgatc gccaccgagt cttgggtcgt gatggggaag atgctcccgt cgacgtcgag
      661 atggacgaac gcgtagacaa gcgcgctcac caacgtcgtc gacaccgcca gcccgaaacc
      721 ccacccggca gagaccacca ggacgccgag caggaagacg acaccaaagg gcatccccgg
      781 aaagatcttc tcgagcagat acaccagaag tgtctccgca gcgatgaagc acgcggctac
      841 cacgatgccg accgcgagcg gcggggccgt cgggcgcacc aacagcccgg ccaggcgggc
      901 gcccgtcagg cgcgaccccg ctctcgcatc acaactgcgc gccacatgca cgtcgaccac
      961 agggacagcg tcaggtcgcg aagacggtca agtgatcgcc gggtggcgat gcggtggtgg
     1021 cggccgccgt gacggcagcg gcgacgtcat cgtcgccatg gtccccggca atggcctcgt
     1081 accatcgcgg tagtagagcg ctcgtcagtg gcagctccac accgtgccgg tgggcggcgg
     1141 tcagtgcgag ctccgcgtcc ttggtcgcgt gtcgcaacgg gaagccgggg acgaagtcgc
     1201 cgttgagcat cgcggcgccc tttgcgagcg cgtagtgcga acccatgggc ccgtctccaa
     1261 ccgcctcgag caggagttgc gggtcgagac cgagtgcttc actgagcgtg agggtttcga
     1321 ccatcccttc gacaagcacc gccaaccagt tgttgagtgc gagcttgagt cggctgccgt
     1381 caccggcatg ctgcagccac agcgtttgat gaccgagcac gtcgaagatc ggctccaccc
     1441 tggaacgaac cgctgatgca ccggaagcga ggatgacgag ttcgccttcc tccgcggcgc
     1501 cggagctgcc cgacactggg gcgtcgacga acgccacgtc atgcctggcc accaggccgg
     1561 cgaaccggtc agtccattcg gcaccgaccg tgcccatctg gatccagatc gcccccgctg
     1621 gaagcattga cagcgcacct gtcgggccgg tcatgacctg ctcgacggcg gcaccgtcgg
     1681 cgagcatggt gatcaacaca tcggcgccgg cggcagcatc ccctggagtg gacatgcggc
     1741 gtgcatcgac gcccaccact gcggccttgg ccagagtgcg gttccacacg cgtacgtcca
     1801 acccggcacg gagcaggttg cgggccatcg gagcgcccat cgtcccggtg cccagcacgg
     1861 tcaccgccgg ccgagtcatg gcataccttc cggggacggt ccggcgaccg gtgtggcgtc
     1921 gtccatgatc gtttcgatct catcgagatc cgagtcggta agcgagatgt cgacggcacg
     1981 gagactgtct tcgatgtgat cgacgtgccg agcaccgacg atcgcgacgt gcacggccgg
     2041 gttcgccagc gtccacgcaa tggccaactg actgacgctg ataccgcggt cggcggcgaa
     2101 cttctccaac gtccgcacgg ttgccaggtt gcgccggaat gtgccgccgc tgaagacccc
     2161 gctcgttgcg cgccagtcat cctcgtcaaa ggcggtgtgc acgctgagcg tgcccgtcag
     2221 cagcccgtga gccagcgggc cgtacgccaa cacgccgatg tcgtgcgcgc ggcaatatgg
     2281 cagtacctgg tcctcgatct ctcgacggaa caggtgatac ggcggctgca gcgtctcaac
     2341 cggcagtgtc gccgagaatt cggccatctg ttcggagtcg taattcgaga cgccgacatg
     2401 gccgatcttg ccttcggtga ccaggtcgcg cagggcgcct gccgtctcgg ccacaggcac
     2461 cgtcgcatcc ggccaatgca cctggtagag gtcgatgtga tcgataccga gcgctgtcag
     2521 gctcgcgtcg acccctttgc gcagccactc cgggcttgcg tcgcgaacga tgccggaatc
     2581 gatttggcgc aggccgcctt tggtggcgat gacgagctcg ttgcggttac cgatgagctg
     2641 gtggcgcaac gctctgccga ggatgcgctc agacgcgcca aagccgtacg cctgggctgt
     2701 gtcgaagaag ttgacaccca attcgcgggc gccgcgaatt gccgcgacgg ccgtggcctc
     2761 gtcgaactgg ccccagtcgc ccccaagctg ccaagtaccg aatgcgattc gggacacttc
     2821 caggccgctg cggcccaatg tcgtggtctt catgccgttc caatctcatc gggccttggc
     2881 aggcccgcgt ttgcgtggtt gttgccagcg ttccgcacgg gcgtaacggt gtcgctaccg
     2941 ctgataccaa tggcagcccc ccggcaggga gaatctgggt atacctgcca gctggagttg
     3001 gtacccccgt gtgagatgtt tgcagttagt gggaatgcat acctgtacta aagactgcga
     3061 agctgacctc atgctgctgc gaggtttccg atgcgctgtc taatcgttga tgacagcgcg
     3121 ggtttcatcg acgccgcgcg cggcctgttg gagtgtcagg gcttatcggt cgtcggcgtg
     3181 gcgtccacca gcggcgaggc gctgcggttg ttcgaggaaa tacggcccga cgtgacgctg
     3241 gtcgacatca acctcggcag ggagagcggc ttcgagctcg ctgagcaact ccatcaggtt
     3301 ggggggtcga caccgtcacc ggtgatattg atttccaccc acgccgccca agacttcgcg
     3361 gacatgatcg aaaccagccc cgccgtcggg ttcctatcca agtccgcctt gacgggcgcg
     3421 gccatccgcg atctcgtcga cgggttagca agcgtcgagg aaggtgatca ccgctaacac
     3481 ccggcggtga tcctcgccgg cctccggcag gttcagcttg gtcaagatgc tgcggacgtg
     3541 cttttccaca gtgccttcgg tgacccaaag tcgatgagcg ataccggcat tggaacgtcc
     3601 ctccgccatc aacgccaaca cctcacgctc cctcgcactg agggccgcta acggatcatc
     3661 ccgcctccgc gccgacacca gctcggacac caacgctggg tcgaccaccg acgcgccctt
     3721 ggcgatccgc tccaacgttt ccaggaaatc cgccacatcg gtaacccggc tcttcagcag
     3781 ataaccgatg ctgtggccgc tgcccaataa ctccatcgca tgctcgacct caacatgcgc
     3841 cgacagcagc aagatggcga tatcggggaa ctcctcccgg atcacccgcg ctgcatcaag
     3901 cccctcagtg ctatgcgttg gcggcatccg aatgtcggcc accaccaact ccggggtgcg
     3961 ctcgcgaacc agcgcgagga gctggccacc gtcaccggcc tgcccaacca catcgaaccc
     4021 cgaccggtcc agcagactgg ccaaaccctc tctgagcaac aaatcgtcat cggctaccac
     4081 tacctgcatc gcgatcacca ccttccgggt cggctcgaac tcatactgca ccgttcggat
     4141 accgatgcac ctgaggattg agcaaaactg aagctggcgg gtatgccaca gtcgggctgg
     4201 caggggcgct cagtgcgcga actgctcctc caccgcgcgc ctgtcgagtg cgcccttcgc
     4261 agtatgaggc agagccgcga cgacttcgag ccgttcgggc acttcgaaag gcgaaagctt
     4321 gctcttgcac tcccgcagga ttgtctcggg tccgatgctc tcgttctggg acaggactac
     4381 gaccgcgccc acacgctccc cgtacgtcgc gtcgggcatc gcgaaaacgg ccgcctcggt
     4441 gacaccacga cacccggcga ggaccgcctc gacgtgctcc ggcgagatct tttcgccacc
     4501 gcgattgatg atgttcttga tgcgcccggt gagaaacagg tacccgtcct cgtcgactga
     4561 ccccagatca cccgtgcgga accatccttc ggcgaagctg tgcgccgtgc cggcctggtt
     4621 ggcgaggtac ccgcgcgcga ccgtgggacc gtgtacccac acctcgcctt cgatgccggc
     4681 cgggcagatg tgcccgtcgt tgtcgacgac gcgaaggtcc accccggttg cccggccgac
     4741 cgatccgtgc ttaacgggtc cacgcagcgg cagcggcagg ctggtcgcct gatgcgaggt
     4801 ttcggtcatc ccgtaggcgg acaacacggg cgcgccgaag gtgcgctcca tcgcccgcgc
     4861 cgtgaccgtg tcgaggggcg cactgcagct gcgcacgaac ctcaacggca cttcctgctg
     4921 gccggggtac tcgcgcggtg accgctgcag aaggatctga tggatggtgg gaaccgcggt
     4981 gaaccaggtg gcgggcgcgg cccgcatgtc gtcccagaag gtacgtgcgg agaatcggcc
     5041 gtgctcaggg agtagcacac atccgccgct ggcaagggtg gacagcagca ccgcgagcaa
     5101 gccgtgtcca tggaagagtg gcatcaccgc gaccgtcgca tcgtcgggac ccagctcgta
     5161 gctcccgcaa atggccctca cggaggcggc gaggttcgca ttcgtcagcg gcaccatttt
     5221 ggcctggtct gtggtgcccg aggtgaacag gacaatggcg tcgtcgtcag agagatcgtc
     5281 taccggaccc cggacgcgtc gccctagact cgcgccgatt tcgagggtgg ccgtcgtgat
     5341 cccgccaccg gcaacgtcta cccgcacttc gcatgtcgca atgggaaact gcgccggtgg
     5401 cgcggaatcg accgcgcgtg ggccgacgag gatgccctgt gcccccagcc tctcgagccg
     5461 ggcggccatc tccgactggg gcagtgcggg atccagggga gcgatcacca gcccggcgcg
     5521 tgccgcccca aggagcgcca cgacgaactc gacattgttg gcactgatga ggccaacggc
     5581 gtcccctctg cgcagccctt tactgcggag gcggccggcc atgtcgttga ccaacgcgtc
     5641 gagggcgcca taggacagag gtatccgatc cttcgttaag acaagcgcct gtccttccgg
     5701 acgatcgatc acctgccgat cgaagagatc gaccaatcct gtgatcgccg gcgccacata
     5761 catactcgtg ccgtcaacga ctcgctcagt gagagcggcc atggccacct cctcgtaaac
     5821 ggtcatgcag tcgcttgcta taagcctggt gggggacagc cggcacgtgc aataccgact
     5881 cgcgagcggt cggtaacgga cggctatcgc gctcgagcgg cctgaattga ggcggcacga
     5941 ccggcgtggt cagcgtccca cgcgcgaggt actggtcgag atttcgcaac accagctccc
     6001 tcatcatcga gaggcttcgc actgtcacgc cgccgatgtg tggtagcagc accacgttgt
     6061 ctagtcgaca taattccgtt ggaacataag gttcgtcggt gaaaacatcc agtcctgcgc
     6121 ccgccagccg ccccgcgaca agcagttcga cgagggcatc ctgatcaacg acgcttcccc
     6181 gcgcgatatt gatgacatag ccgtgcggcc cgagagcttc caagaccgca cggcttacaa
     6241 ggtgttcggt cccagcgcct ccaacggttg ccaccaccag cacgtcaacg gactcggcga
     6301 gcgcaaccgg cgaggcgagg tagaggtagg gagagccggg cacctcgcgg cggttgtggt
     6361 acgcgatggc acagccaaag gcgaccagcc gcgcggcgat cgctgagccg atgcggccaa
     6421 ggcccaagat gccgactcgt gatccgctca cgtcacggcc cagagcgtac tttccgtcat
     6481 tgggccaatt gccggcgcga acatggcggt ctgctgtgca caagccacgc atcgtcgcca
     6541 gcatcaggcc gacggctgtg tcggcaacag agtcgttgag cacgtcgggg gtgttgctga
     6601 ctccaatacc gagccggtac gccgcgtcga cgtcgatggt gtcgtagccg gctccgtggt
     6661 gcacgatggc gccgaggttg ggcagcgcct tcatcagttc cgcgtccaca ccaggggggc
     6721 cagagtcgac gatggcagtg accgaccgtc catgctcggc aagaaagtcg gctcgtgtgg
     6781 catcgttagg taattgcagc acttggtgtc tcgccgccag tacgtcggcc agtgacggct
     6841 c
//
LOCUS       DASYLX010000234         9874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3081846,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000234 DASYLX010000000
VERSION     DASYLX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081846"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VGP27_05745"
     CDS             complement(<1..720)
                     /locus_tag="VGP27_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7850851.1"
                     /translation="MSSPQVGRGSSLGLTVGATNLACTGDGHRPLIRPSVVTLEPGLT
                     LTGFVDRVGDPVPMVAQDGSRHRPELLLAGALDGVARNAAKAPVSEVAVAVPAHWRAS
                     MVEALRTALRSKPGLTGAPLVSDAAAALTALRSNPGLPSAGVIVLCDFGGSGTSITLV
                     DAGAKDIPIGETVRVQDFSGDQIDQAVLTRVVAGIFEASGADPAGTSMVGSLGRLRDE
                     CRRAKERLSDETATAIVVDVPG"
     gene            complement(870..3389)
                     /gene="iniR"
                     /locus_tag="VGP27_05750"
     CDS             complement(870..3389)
                     /gene="iniR"
                     /locus_tag="VGP27_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoniazid response ATPase/transcriptional
                     regulator IniR"
                     /protein_id="HEV7850852.1"
                     /translation="MAGPMPEALTDIPPAAREAVSALTAAPAEPVRMLVSGGIGTGKS
                     SVLAMVRNTLRSADIQVLTRAPRGGDDPASAIVIDDAHLLADEELEQLVERASDPGST
                     VIVAAEPLAQRRALRALATALERENPVISLGSLTSPEVTETAALVTGGHPASEIVRAL
                     MLSTAGLPFLLRPAMAAAVSPNGEPAANAIVEAARFALIERLRRVEEPVLDTLLVSSL
                     SPDLGPDDVAAALRLRSEEAQRLVDRARATGLIEPSHSQTFLRSVHLGVAAIIGAARH
                     HDIEISLLRSQIELSTLSSDLALRMAEHGLRDDRLANALTDLASHHRSQPAEAARLYR
                     AAAEAGATAVSARLADALAMTGDCATAGRLTDELLASEDAAERAAAVRIAASIAAHDG
                     GAAQAADLFRWLGPYPDAFIAAANAVVSIAAGDAQAARAALSAESAGPPTSTTRAAHS
                     LAEGLLLSLDQPFPVAVARLGQAITTEQQQLGVVPDSPAALVTLAALHGGDPVRARSV
                     IGRAVRAGGEDDAFSAHRHRLLLGWVRMQDGQLGPAMADAASVGDAALHRRDALWAAA
                     LNTAIARRSGDGGAVQKHWYAAMEVLAEYSVDLFALLPLGELWVAAARMRQVDWLQHA
                     LDEAFALLASLGSPVLWSVPLHWAGVHAGILANSPEAVAPHGHALTAAAGSSAFAKAL
                     SGAGRTWLRVLANHVDADEVTAAARSLSQFGLTSDATRLAGQAALQTPDGRVSGAMLQ
                     LARDLKLTTAIDDGPGVESMPTATAPAGSGRQQSSRLSDREREVAELLLLGMPYRDIG
                     SQLFISAKTVEHHVARIRRRLGADSRSEMLSMLRAMLATPG"
     gene            complement(3402..5192)
                     /locus_tag="VGP27_05755"
     CDS             complement(3402..5192)
                     /locus_tag="VGP27_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7850853.1"
                     /translation="MAEALGLSIGATILVAARVGRPPVMRRAVLTLFAGRAPEVGVPT
                     ENPALNQPGVVLTNFVERVGDPVPMVASDGSSHRGERVLAEALDGMARTVGGGQPVAI
                     AVPAHWGPAVIGALRGALREKPNLAPGGVPAALIPDSIAALAALQAAPGLPAGVIALV
                     DLGGSGTSITLAEATATLTPIGETLRYADFSGDEIDQAVLNHVLAGVADANNADPAGT
                     AAVGSLSRLRDESRQAKERLSAETATAVPVDLPGFRSDVRLTRTELDNLIAGPLAGLL
                     AAIEEVLARNNVAPADLAAVATVGGGAGIPLITQRLSEQFRVPVITTPHSQLNMAAGA
                     ALLADQSLDADAPTGLSTAADAATGIAPAAWAAGAAGLAASESAADGAPSATFRALAW
                     SQDDAAGSEPVPYTGGDYTADVYSGPTDARPPIDFATAGEPPYEPEPAPLPWYKRPPI
                     LFGAAAVAALLAIGGLAITLTGNSGVLGPVTETSTTFSLEPTSGPPPAPVEPTTVTVT
                     GANGQPTTTVVTPPPPSPSATSTTTTTSPTTTSSSTTTTTTTTTTTTTTTTTTTTTTT
                     TTSPPAPKPQPPPQPQPQPEPQPNPPAGGA"
     gene            5433..5975
                     /locus_tag="VGP27_05760"
     CDS             5433..5975
                     /locus_tag="VGP27_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv0340 family IniB-related protein"
                     /protein_id="HEV7850854.1"
                     /translation="MANPLLDFVMSVVRDPDVAAQYAADPAGAIHAANLTDVTSADVN
                     ALIPVVSESLSTALPTSGAESLIGDAAGNVWSSGAATAAFDAFDDHVPAPAIDDGHTI
                     ASNVIDEPADSLHAGVDTLHDAGVPMVADIEDPSAQLTGGIIDDAPLVGDATDADWHS
                     VVDDAAHHLDAGDTGFDIFD"
     gene            6240..7256
                     /locus_tag="VGP27_05765"
     CDS             6240..7256
                     /locus_tag="VGP27_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850855.1"
                     /translation="MSNPISFLFDWLLGLTRPGADPSDVRAFVANPTQAASQAMGTAV
                     TQAQLTQAASAVVAPAAVAGGGNAIQALQHTVADNNGLTFAPQRVFAPETNTDFASHN
                     DIGSPRAGEDVQQGVGNFDLDFDFSRDINASNGAVVNTGNAGNIDTTNVHGSGNVVGD
                     HNDGNNTGDVHAGQGSNVNAGGRNNDLHDSSHDVNTTAGGDVATNTGSGQNTQVGDID
                     SSGGGAAGGHGGHGGGLISVGSDGGDGGGAAGGSGGGVIIAPAQNSSSSNQSAGDDIN
                     HVNASGSHAPVSVGNEDDHSVGNVGGDVTQVDHVNGNVDASHDDNSVHHQTDIHQGVA
                     VDLF"
     gene            7339..9186
                     /gene="iniA"
                     /locus_tag="VGP27_05770"
     CDS             7339..9186
                     /gene="iniA"
                     /locus_tag="VGP27_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoniazid-induced dynamin-like GTPase IniA"
                     /protein_id="HEV7850856.1"
                     /translation="MTQPNDPRKVTVILELIDHTKKIAEANQRGDLAERLERARIRIS
                     DPQIRVVIAGQLKQGKSQLLNSLLNVPVARVGDDETTVLATVVSYSEQVSAQLIVAAP
                     DGAEPAVIPIPPASLTSDLRRAPEAGGREVLRVEVTANSPLLKHGLAFVDTPGVGGHG
                     QPHLSATLGLLPDADAMLMITDTSQEFTEPELTFIRQAHEICPVATIVATKTDLYPHW
                     RQIVAANQAHLQRAGLSIPVIPASSLLRGHALTHNDKELNEESNFPAIIKFLSERVLS
                     RQNDRIRDHVLAEIRSAAEHLTLKADSELSALTDPGTRDRLKDELERRKEDAQEALQQ
                     TALWQQVLNDGIADLTSDVDHDLRARFRAITQHTEKVIDDVDPTNHWAEIGTELEDAV
                     ATAVGDNFVWAYQRAEALAEEVGRTFVEAGLDAVKMPQIDAREMGAGFGELKSLARLE
                     AKPIKAGHKIVTGMRGSYGGVLMFGMLTSFAGLGMFNPLSLGAGLVLGRKAYKEDMEN
                     RMLRVRNEAKMNMRRFVDDVAFVVGKESRDRLKGVQRQIRDHYRGIANQATRSLNESL
                     QATLTAAKLEENELNSRVAELERQLNILGQVTDHATKLAVAAPQSVASG"
     gene            9243..>9874
                     /locus_tag="VGP27_05775"
     CDS             9243..>9874
                     /locus_tag="VGP27_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEV7850857.1"
                     /translation="MSTSDQVRAILGGTISAYRADPAYLQRPDVHNELDRIGRRLNQP
                     IRIALAGTLKAGKSTLVNALVGEDIAPTDATEATRIVTWFRNGPTPKVTANHRGGRRS
                     NVPIARNGGLTFDFARLDPQDVVDLDVEWPAAELIDTTIIDTPGTSSLSRDVSQRTLQ
                     LLVPDDGVPRVDAVVFLLRTLNAADIALLKQIGELVGGSSGALGVIGVASR"
BASE COUNT         1606 a         3432 c         3382 g         1454 t
ORIGIN      
        1 ccccggcacg tcgacgacga tcgcggtggc cgtctcgtcc gacagccgct ccttggcgcg
       61 cctgcactcg tcgcgcagcc tgccaagcga tccgaccatc gacgtgcccg ccgggtcggc
      121 gcccgacgct tcgaagatcc ccgccaccac ccttgtcagg actgcctggt cgatctggtc
      181 gcccgagaag tcctgcacgc ggaccgtctc gccgatcggg atgtccttcg ctcccgcgtc
      241 gaccagcgtg atgctcgtgc cgctgccgcc gaagtcgcac aggacgatca cgcctgccga
      301 cggcagtccc gggttcgacc gcagtgccgt caaagccgcg gcggcgtcgg acaccagtgg
      361 tgcccccgtc agcccgggct tgctgcgcag cgcagtcctt agcgcctcga ccatggatgc
      421 gcgccagtgc gcaggcaccg cgacggccac ctccgacacg ggcgccttgg cggcgttgcg
      481 tgcgacaccg tcgagcgccc ctgcgagcag cagctccggg cgatgccggg aaccgtcctg
      541 ggcgaccatc ggcactggat cgccgacgcg gtcgacgaac ccggtgagcg tcagacccgg
      601 ttccagggtg accaccgagg ggcgaatgag cggtcgatgc ccgtcgccgg tgcaggcaag
      661 gttcgtggcg ccaacggtca ggccaagcga actacctctg ccgacttgcg gactcgacat
      721 gtcacacctc tacctcggat cggtaacgaa acgcctggct cgaccctatg gcgcaggacg
      781 cggcggcgga tcggtgctca ccccggtgcc cggatagtcc ccttggggga tctcgattag
      841 gggattcgaa tgcgttaggg gattcgcggt cagccagggg tggccagcat ggcccgcagc
      901 atcgacagca tttccgaccg ggaatccgcg cccagcctgc ggcgtatgcg cgccacatga
      961 tgctcgacgg tcttggccga gatgaagagc tggctgccga tgtctcggta gggcatgccg
     1021 agcagcaaca gttcggcgac ctcgcgttcg cggtcggaca accgtgacga ctgctggcgg
     1081 cccgagcccg ccggcgcggt cgccgtcggc atcgattcga cgcccggccc gtcgtcgatg
     1141 gcggtcgtga gcttcaggtc gcgggccagc tgcagcatgg caccggacac ccgaccgtcg
     1201 ggggtctgca gcgccgcctg gccggccaac ctggtggcgt ccgaggtaag gccgaactgt
     1261 gacagcgagc gagccgcagc ggtgacctca tcggcgtcga catggttggc cagcacccga
     1321 agccatgtcc ggcccgcacc cgagagcgcc ttcgcgaacg cactgctgcc tgcagcagcc
     1381 gtcagggcat ggccgtgcgg cgcgacggcc tccggagagt tggccaggat tccggcgtgc
     1441 acgccggccc agtgcagcgg caccgaccac agcaccgggc tgcccaagga ggcgagcagc
     1501 gcgaacgcct cgtccagtgc gtgctgaagc cagtcgacct ggcgcatgcg cgcggcggcg
     1561 acccacaact cgcccagcgg aagcagcgcg aagaggtcga cggagtactc ggcgagcact
     1621 tccatggcgg cgtaccagtg cttctggaca gcgccgccgt caccgctgcg gcgggcgatg
     1681 gccgtgttca gtgccgccgc ccacagggca tcgcggcggt gcagtgcggc gtcaccgacg
     1741 gacgcggcgt cggccatcgc gggacccagc tggccgtcct gcatccgcac ccagccgagc
     1801 agcagccggt gccgatgggc cgaaaacgcg tcgtcctcgc cgcctgcgcg caccgcacgc
     1861 ccgatcacac tgcgcgcgcg gaccgggtcg ccgccgtgca gagcggccag cgtgaccagt
     1921 gcggccggac tgtccggtac gacgccaagc tgttgttgtt cggtggtgat ggcctgcccg
     1981 aggcgggcca cggcgaccgg aaacggctga tccagtgaga gcagcaggcc ctcggccagg
     2041 ctgtgcgcgg cccgcgtggt tgacgttggc ggaccggcac tttcggcgct caacgcggca
     2101 cgggcggcct gcgcgtcacc cgccgcgatg gacacgacgg cgttcgcggc tgcgatgaag
     2161 gcgtcggggt acgggccgag ccagcggaac aggtctgcgg cctgcgcagc accgccgtcg
     2221 tgtgcggcga tgctcgccgc gatgcggacc gccgccgccc gctcggctgc gtcctcggag
     2281 gccagcagct cgtcggtgag cctgcccgcg gtcgcacagt cacccgtcat cgccaacgca
     2341 tcggcgaggc gggcgctcac ggcggtggct cccgcctcgg ctgccgcgcg gtagagccgc
     2401 gccgcctcgg ccggttggct ccggtgatga gaagcgaggt cggtcagtgc attcgcgagt
     2461 cgatcgtcac gcaggccgtg ctcggccatt ctcagcgcga gatccgatga cagcgtggac
     2521 aattcgatct gagagcgcag caaggagatc tcgatgtcgt ggtgacgggc agctccgatg
     2581 atcgcggcaa cgccgaggtg gaccgagcgc agaaacgtct ggctgtgtga gggctcaatg
     2641 agaccggtgg cgcgggcacg gtccaccaac cgctgcgcct cctcggagcg caggcgaagc
     2701 gccgccgcaa cgtcgtcggg cccgagatcc gggctcagtg acgacaccag aagggtgtcg
     2761 agcaccggct cctcgacgcg gcgcaggcgc tcgatcaacg cgaacctggc cgcctcgacg
     2821 atggcgttgg ccgccggctc gccgttgggt gacactgccg cagccatcgc gggacggagc
     2881 agaaacggca gaccggccgt cgaaagcatc agcgcccgaa cgatttccga cgccggatgg
     2941 ccgccggtca ccagcgccgc cgtctcggtg acttccggtg aggtcagcga gccgagcgat
     3001 atcaccgggt tctcgcgttc gagggcagtg gccagcgccc gcagcgcccg ccgttgagcg
     3061 agcggttcgg cggcgacgat cacggtcgag ccaggatcgg acgcgcgctc gacaagctgt
     3121 tcgagttcct cgtcggcgag caggtgcgcg tcatcgatca cgatggcgga cgcgggatcg
     3181 tcaccgcccc gcggtgcccg tgtcagcacc tggatatcgg ccgaccgcaa cgtatttcgc
     3241 accatcgcaa gcacggaact cttacccgtg ccgatgccgc ccgacaccag catcctgacc
     3301 ggctcggccg gtgcagccgt cagcgccgac accgcctcgc gggcggccgg cggtatgtcg
     3361 gtcagtgctt caggcattgg cccggccatc cccgttaccc gctatgcccc accagcgggc
     3421 ggattgggtt gcggctcggg ctgcggctgc ggctggggcg gcggctgcgg cttcggtgcc
     3481 ggaggtgacg tcgtggtggt ggttgtggtg gtcgtcgtcg tggtggttgt ggttgtggtg
     3541 gtcgttgtgg tggtcgttgt ggtgctgctg ctggtcgtcg tcggggacgt ggtggtggtg
     3601 gtgctcgtcg ccgacgggga aggtggtggc ggtgtgacga cggtggtggt gggctggccg
     3661 ttggccccgg tcaccgtgac cgtcgtcggc tctacgggtg cgggtggcgg ccccgatgtc
     3721 ggttcgagcg agaaggtcgt actcgtctcc gtcaccggcc cgagcacacc gctgttaccc
     3781 gtcagcgtga tcgccaggcc gccgatcgcg agcagcgccg caacggctgc cgccccgaac
     3841 aggatcggcg gtcgtttgta ccagggcagg ggagccggct cgggctcgta gggcggttcg
     3901 cctgcggtag cgaagtcgat cggcggacgg gcgtcggtgg gcccggaata cacgtcggcg
     3961 gtgtagtcgc cgccggtgta gggcaccggt tcgctgcccg ccgcatcgtc ctgcgaccac
     4021 gccagcgcac ggaacgtcgc cgagggggcg ccgtcggccg ccgactcgct ggcggcgagc
     4081 ccggccgccc cggccgccca ggccgcgggc gcgattccgg ttgcggcatc ggccgccgtc
     4141 gacaatccgg ttggtgcgtc ggcgtccagg ctctggtcgg ccagcagtgc cgctcccgcg
     4201 gccatgttga gctgcgagtg cggcgtcgtg atcaccggga cacggaactg ctcggaaagc
     4261 cgctgggtga tcagcgggat acccgcgccg ccaccgacgg ttgccaccgc tgcgaggtcg
     4321 gcgggggcga cgttgttgcg cgccaacact tcttcgatag cagcgagcag accggcgagc
     4381 ggtccggcga tgaggttgtc gagttcggtg cgcgtcagcc ggacatcgga ccggaagccg
     4441 ggcagatcga cgggcaccgc ggtggcggtc tccgctgaaa ggcgctcctt ggcctgcctg
     4501 ctctcgtcgc gcagacggct cagcgagccg acagcggcgg tgcccgccgg gtcggcgttg
     4561 ttggcgtcgg cgacaccggc caggacgtga ttgaggaccg cctgatcgat ctcgtcgccg
     4621 gagaagtcgg cgtacctgag ggtctcgccg atcggtgtga gggttgcggt cgcctccgcc
     4681 agcgtgatgc tcgttccgct gccgccgagg tcgacgagcg cgatgacgcc ggcaggcagt
     4741 ccgggtgcgg cctgcagggc ggcgagcgcg gcgatggagt ccggtatcag cgcggcgggc
     4801 acgccgccgg gcgccagatt cggcttctcc cgcagggctc cgcgcaacgc accgatcacc
     4861 gccggtcccc agtgcgccgg caccgcgatg gcgacaggct gtccgccgcc gaccgttcgg
     4921 gccatgccgt cgagggcctc ggccaacacg cgttcgccgc ggtgtgagct gccgtccgag
     4981 gcgaccatcg gaaccgggtc gccgacccgc tccacgaaat tggtcagcac gacaccgggc
     5041 tggttgagcg ccgggttctc cgtgggcacg ccgacctcgg gcgcccggcc ggcgaacaat
     5101 gtcagcactg cgcggcgcat caccggcggg cggccgacgc gcgcggcgac gaggatggtc
     5161 gccccgatgg acaacccgag agcttcagcc atgctcggct ccccctcgct ccggtttcat
     5221 gcacactctt ctgtgttcgg ccgcccatca cgatagcggc cgagcccacc gctttccccc
     5281 taatgtcgcg gaacccctaa cggcgagtcc cctaacgccc cagctgcaga gaagggtttc
     5341 gcaccgatca cgccgcactt cggcctggat agcatcgtga ctaaccgacg ggcgcccgcc
     5401 cccagcaatc gcgatcggaa agaaggtgcg tcatggcaaa cccgctactc gacttcgtga
     5461 tgtcggtggt gagggaccct gacgttgccg cccagtatgc cgctgatccg gccggggcca
     5521 tccacgccgc caatctgacc gatgtgacca gtgccgacgt caacgcgctg attcccgtgg
     5581 tgtcagaatc gctgtcgacg gcactgccga cctccggcgc cgaatcactc atcggagatg
     5641 cggccggcaa cgtatggagc agcggggctg cgaccgccgc gttcgacgcg ttcgacgacc
     5701 acgtgccggc tccggccatc gacgacgggc acaccatcgc atcgaatgtc atcgacgaac
     5761 ccgccgattc gttgcatgcg ggtgtcgata ccctgcacga cgccggtgtc ccgatggtgg
     5821 cggacatcga ggatccgtcg gcgcagctca cgggcggaat catcgacgac gctccgctag
     5881 tcggtgacgc caccgacgcc gactggcatt ccgtcgtcga cgacgcggcg caccacctgg
     5941 acgccggcga caccggcttc gacatctttg actagaccca cgagcatccc gaggacggcg
     6001 cggccatcga cggtcgcgcc gttttcgtct gtcaaagccc gggtgagctg ggtaaatgca
     6061 cctaccccca ccacccccta atcccctaat gggggatccc ccaagatccc ggcacggggt
     6121 cgcatgggga ggggtgccga ccccgtttcc gcggggttga cgggtccata acgtagtccc
     6181 cagatcgccg gacagcccgg tgacgaccac agatcaacac tcaccgaaag gcactcaaga
     6241 tgtcgaaccc catctccttc ctcttcgact ggctcctcgg gctgacccgc cccggtgccg
     6301 atccctccga tgtccgcgcc ttcgtcgcca acccgacgca ggcggcttcg caggcgatgg
     6361 gcaccgcagt cacccaggcc cagctgacgc aggccgcatc cgcggttgtc gctccggcgg
     6421 ccgtggccgg cggtggcaat gcgatccagg cgctgcagca cacggtcgcc gacaacaacg
     6481 gcctgacgtt cgccccgcag cgggtgttcg cccccgagac gaacaccgac ttcgccagcc
     6541 acaacgacat cggcagcccg cgcgccggtg aggacgttca gcagggtgtt ggcaacttcg
     6601 acctggactt cgacttcagc cgtgatatca acgcctccaa cggcgccgtc gtcaacaccg
     6661 gcaatgccgg caacatcgac accaccaacg ttcatggcag cggcaacgtc gtcggcgacc
     6721 acaacgacgg gaacaacacc ggcgacgtgc acgccgggca gggctccaac gtcaacgcag
     6781 gcggcaggaa caacgacctg cacgacagca gccacgacgt gaacaccacg gcgggcggcg
     6841 acgtcgcgac caacaccggc agcggccaga acacccaggt cggtgacatc gactccagcg
     6901 gtggcggagc cgcgggcggc catgggggcc acggcggcgg cctcatcagc gtcggcagcg
     6961 acggcggtga cggtggcggt gcggccggcg gttctggcgg cggcgtgatc atcgcccctg
     7021 cgcagaactc cagcagcagc aaccagtctg cgggcgacga catcaaccac gtcaacgcgt
     7081 cgggttccca cgccccggtg tccgtcggca acgaggacga ccactcggtc ggcaacgtcg
     7141 gcggtgacgt cacgcaggtc gatcacgtga acggcaacgt ggacgccagc cacgacgaca
     7201 actccgtgca ccaccagacc gacatccacc agggcgtggc cgtcgacctg ttctgaccgg
     7261 gagcaacaac gcccatagcg cagctggcgg ggatcgattc cggtccccgc cagttcgcac
     7321 gtctaccgta ggggcaccat gacgcaaccg aatgacccgc gcaaagtgac ggtcatcctc
     7381 gagctgatcg accacaccaa gaagatcgcc gaagccaatc agcgcggaga tttggcggag
     7441 cggctggaga gggcacggat ccggatctcc gacccgcaga tccgcgtggt gatcgccggc
     7501 cagctcaagc aggggaagag ccaattgctc aactcgctgc tcaacgtgcc ggtcgctcgc
     7561 gtcggcgacg acgagacgac ggtgctcgcc acggtggtgt cgtattccga gcaggtatcg
     7621 gcccagttga tcgtggcagc acccgatggc gcggagcccg ccgtgattcc gatcccgccc
     7681 gcgagcctca caagcgacct tcgccgcgcg ccggaggccg gtggccgcga ggtgctgcgg
     7741 gtggaggtca ccgccaacag ccctctgctc aagcacgggt tggcgttcgt cgacacgcca
     7801 ggcgtcggcg gccacggcca accgcacctg tcggcgacgc tgggcctgct gccggacgcc
     7861 gacgccatgc tgatgatcac cgacaccagc caggagttca ccgagcccga gctgaccttc
     7921 atccggcagg cgcacgaaat ctgcccggtg gcaaccattg tcgcgaccaa gaccgacctc
     7981 tatccgcact ggcggcagat cgtggcggcc aaccaggctc acctgcagcg cgcgggactg
     8041 tccataccgg tgattcccgc ttcgtcgctg ttgcgcggcc acgcccttac gcacaacgac
     8101 aaggaactca acgaggaatc caacttcccg gccatcatca agtttctcag cgagcgtgtg
     8161 ctgtccaggc agaacgaccg cattcgcgac cacgttctcg ccgagattcg ttcggcggct
     8221 gagcatttga cgttgaaagc ggactccgag ctgtcggccc tgaccgaccc cggcacccgc
     8281 gaccggctga aggacgagct ggagcggcgc aaggaggacg cccaggaggc tctgcagcag
     8341 accgcactgt ggcaacaggt cctcaacgac ggcatcgcgg atctgacgtc ggatgtcgat
     8401 cacgatcttc gcgcgcgctt ccgcgcgatc acccagcaca ccgaaaaggt catcgacgac
     8461 gtcgacccga ccaatcactg ggccgagatc ggcacggaac tggaggacgc cgtcgccacc
     8521 gcggtcggcg acaacttcgt gtgggcctac cagcgtgccg aggcgctcgc cgaggaagtg
     8581 ggccggacat tcgtcgaggc cggtctggat gcggtgaaga tgccgcagat cgacgccagg
     8641 gagatgggcg caggcttcgg cgagctcaag tcgctggcgc ggctggaggc caagccgatc
     8701 aaggccggac acaagatagt caccggtatg cgcgggtcct acggcggcgt gctgatgttc
     8761 ggcatgctga cgtcgttcgc cggattgggc atgttcaacc cgctgtcgct gggcgccggc
     8821 ctcgtgctgg gccgcaaggc gtacaaggag gacatggaga accggatgct gcgcgttcgc
     8881 aacgaggcga agatgaacat gcgccggttc gtcgacgacg tcgcgttcgt tgtcggcaag
     8941 gagtctcgcg accggctcaa gggggtccag cgccagatcc gcgatcacta ccgcggcatt
     9001 gccaaccagg ccacccgctc gctcaacgag tccctgcagg cgacgctgac ggccgcaaag
     9061 ctggaggaga acgagctcaa cagccgggtc gccgaactgg aacggcagct caacatcctc
     9121 ggccaggtga ccgaccacgc gaccaagctc gcggtggccg ctccgcaatc cgtggccagc
     9181 ggttagctcg agtaacccca tcggctttgc tcagggccgg tgcctaagct ggttcaccgg
     9241 atatgagcac gagcgatcag gtgcgcgcga tcctgggcgg caccatttcg gcctacaggg
     9301 ccgatccggc gtaccttcag cgccctgacg tgcacaacga gctggaccgc atcggccgcc
     9361 ggctcaacca gcccatccgg attgccctgg ccgggacgct gaaggcgggc aagtccactt
     9421 tggtgaacgc gcttgtcggc gaggacattg cgcccaccga cgccaccgag gccacccgca
     9481 tcgtgacctg gtttcgcaac ggcccgaccc cgaaggtcac cgcgaaccac cgcggcggca
     9541 gacgatccaa cgtgccgatc gcccgcaacg gcggtctcac cttcgacttc gcccgcctgg
     9601 acccgcagga cgtcgtcgac ctcgatgtcg aatggcccgc ggcggagctg atcgacacta
     9661 cgatcatcga cacccccggc acctcctcgt tgtcgcgcga cgtctcgcag cggaccctgc
     9721 aactgctggt tcccgacgac ggcgtgccgc gggtcgacgc ggtcgtcttc ctcctgcgca
     9781 ccctcaacgc cgccgatatc gcgctgctca agcagatcgg cgagcttgtc ggggggtcgt
     9841 ccggggcgct gggggtcatc ggggtggcct cccg
//
LOCUS       DASYLX010000235         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_448590,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000235 DASYLX010000000
VERSION     DASYLX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448590"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VGP27_05780"
     CDS             <1..1279
                     /locus_tag="VGP27_05780"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEV7850858.1"
                     /translation="AVRSIVSDGYRAVRELVDVGAQFDETETGRWSLTREGGHSRRRI
                     IHSGGDATGAEVQRALDHAAATLDIRRNHVALRILQAGGAVSGVYVLNEDGAGIVHAP
                     SVVLATGGLGHLYSATTNPEGSTGDGVALALWAGVPISDIEFIQFHPTMLFDGNAGGR
                     RPLITEAVRGEGGILLDRQGNSITAGVHPMGDLAPRDVVAGAIDARLRATGDPCAYLD
                     ARAIAGFDRRFPTVTAACLAAGIDPTRQPIPVVPGAHYSCGGVVADVHGRTDLAGLFA
                     AGEVARTGMHGANRLASNSLLEGLVVGGRAGQAAAQHAIDAGEARAEPGDVETRFALP
                     RRKLQRAMSRYASVVRDADGLQQLTRELDAADPRDLDCRTSFEDVALTTTARAVTAAA
                     LTRTESRGCHHRSDFPDADPALAHSRLLEAARC"
     gene            1279..2136
                     /gene="nadC"
                     /locus_tag="VGP27_05785"
     CDS             1279..2136
                     /gene="nadC"
                     /locus_tag="VGP27_05785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV7850859.1"
                     /translation="MELSADELTEARRVIARALEEDLRYGPDVTTLATVPADATTTAS
                     MVTRDPGVIAGVDIALLVLDEVLGPDEYRLKYRVEDGAHLDAGGVVLVVEAPTQGLLT
                     AERTMLNLVCHLSGIATTTAGWVEAAEGTNAKIRDTRKTLPGLRALQKYAVRVGGGVN
                     HRMGLGDAALIKDNHVAAAGSVVAALRAVREVAPDLPCEVEVDSLEQLDDVLAENVEL
                     VLLDNFPAWQTQIAVQRRDARAPQTKLESSGGLSLDSAAEYAGTGVDYLAVGALTHSV
                     RVLDIGLDL"
     gene            complement(2133..2717)
                     /locus_tag="VGP27_05790"
     CDS             complement(2133..2717)
                     /locus_tag="VGP27_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850860.1"
                     /translation="MPRPDRSRAALIDTAALLFRRQGYAATGVNQILETADVKAGSLY
                     HHFPDGKQELAAAVVESAGGEIERLLRRFLDSDSAVADIVDGWIDMLIVQLASDQRDG
                     CPIEPIATESVNASSQVREVSARAFAGWSAAVAERLRVDGWTDGGAERTALAVIALIE
                     GALILSRIAGDDAALTAAKVAVRTLVSPARAVHG"
     gene            2782..3810
                     /locus_tag="VGP27_05795"
     CDS             2782..3810
                     /locus_tag="VGP27_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV7850861.1"
                     /translation="MQQLVFEEAGRYAWREAADPELTDARQALVRPLMVAGCDLDVAV
                     AEGVLPMPPGHAVGHEGVAEVVAVGDEVASVRVGDRVVVPFQINCGDCNACRRDVTGS
                     CASLPLMAMYGMAPLAGVDGGGFMADLVMVPFADAMLLRVPDGVDQAAIASLSDNIPD
                     GWRAIGPYRGELAALNEVDRRMLVVGRRSIGLYAAAFGSAYGAQVDYVDTDEGRLAMA
                     EKLGARVHERPRPDKSWDPYPVTVHTSADPAVLAATLAATWADGVCTDTGIYYRGAVE
                     MPLLAMYTRGVRFVTGRVNARAVIPEILELLAAGCDLSPAVERVVPWDDAPEVWPTMT
                     GKTIYARV"
     gene            complement(3826..4464)
                     /locus_tag="VGP27_05800"
     CDS             complement(3826..4464)
                     /locus_tag="VGP27_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV7850862.1"
                     /translation="MAWEVWLTFFGAAIAIAVSPGAGAIQSMATGLTHGVRRGYWSIL
                     GLEIGLMLQLALVALGLGAIVTNSILAFNIVKWIGVGYLIYLAVRQWRSATVDLREQI
                     GQPTSGGRLSLVFRGFLVNATNPKGLVFFLAVLPQFVVPNVALLPQYLAIGVTMVGVD
                     LVVMGLYAALASRLLKWMHTPRQQTTVNRLFSGLFAAAAVVLSLVRRGSAAA"
     gene            complement(4477..4887)
                     /locus_tag="VGP27_05805"
     CDS             complement(4477..4887)
                     /locus_tag="VGP27_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7850863.1"
                     /translation="MSAKDHPNNAPGVPMVYPPAFERLQIKYMNPLVKRISRIMPGVA
                     TIRHRGRKSGKTYETVVTPFRKDNVLAIALGHGKTDWVKNVLAANEADVVFGSRTVHI
                     VNPRIVPAGGSAEGLPYMARVQVRKMGVLVADIA"
     gene            5042..5488
                     /locus_tag="VGP27_05810"
     CDS             5042..5488
                     /locus_tag="VGP27_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850864.1"
                     /translation="MALVVALVAAGLALWALLKAPDSQSASTFTGTTTDDPKGSICED
                     FNVIRNGVQINTNLQPPGGPDDVTGSMAVAANARLSLYDGGQYLLARLQPDTPTDLAD
                     AVRKFANDLMDIGARSTSGIPNTDPGQAARLKEADEANATITNLCK"
     gene            5575..>6650
                     /gene="hisD"
                     /locus_tag="VGP27_05815"
     CDS             5575..>6650
                     /gene="hisD"
                     /locus_tag="VGP27_05815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV7850865.1"
                     /translation="MARIDLRGAELSAARLRSALPRGGVDVDAVVPKVRPIVDAVAAR
                     GADAALDYGQSFDGVRPPQVRVPAAELQSALDGLPADVRTALEVAIERTRVVHADQRR
                     TDTTTTLAAGATVTERWVPVERVGLYVPGGNAVYPSSVVMNVVPAQTAGVDSLVIASP
                     PQAQFDGLPHPTILAAARLLGVDEVWAVGGAQGVALLAYGGTDTDGSELAPVDMVTGP
                     GNIYVTAAKRICRSQIGIDAEAGPTEIAILADHTADPVHVAADLISQAEHDEMAASVL
                     ITPSVGLADATDRELAGQLETTVHRKRVTAALTGRQSAIVLVDDVDAGIKTVNAYAAE
                     HLEIQTADPRDVAGRIRSAGAIFVG"
BASE COUNT         1061 a         2200 c         2323 g         1066 t
ORIGIN      
        1 tgcggtgcgc tccatcgtgt cggacggtta ccgcgcggtt cgtgaattgg tcgatgtcgg
       61 agcgcaattc gacgagaccg agaccggccg ctggtcgctg acgcgcgagg gcggccactc
      121 gcggcgccgc atcatccatt ccggtggcga cgccaccggc gccgaggtgc agcgggcgct
      181 cgaccacgcc gcggcgacgc tggacatccg gcgcaaccac gttgcgctgc ggatcctgca
      241 ggccggtggt gcggtgtccg gcgtctacgt gctcaacgag gacggtgctg gcatcgtgca
      301 cgcgccgtcc gtcgtactcg cgacgggcgg cctcggccat ctgtattcgg ccaccaccaa
      361 tcccgaggga tcgaccggtg acggtgtggc gctggcgttg tgggcgggtg tgccgatcag
      421 cgacatcgag ttcatccagt tccatccgac catgctgttc gacggcaatg cgggcggccg
      481 ccgtccgttg atcaccgaag ccgtccgtgg tgagggcggg atactgctcg accggcaggg
      541 caattcgatc accgcaggcg tccacccgat gggcgacctg gcgccacgcg acgtcgtcgc
      601 gggtgccatc gacgccaggc tgcgcgcaac cggtgatccc tgcgcgtacc tcgacgcccg
      661 cgccatcgcg ggtttcgatc gtcgcttccc gacggttacc gccgcgtgcc tggcggcggg
      721 catcgatccg acgcgccaac cgattccggt ggtgccgggg gcgcactaca gctgcggcgg
      781 tgtggtcgcc gatgtgcacg gccgcaccga tctcgcgggg ttgttcgccg ccggtgaggt
      841 ggcgcgcacg ggaatgcacg gtgcgaaccg gttggcgtcc aacagcctgc tggaagggct
      901 ggtggtgggc ggccgtgctg ggcaagcggc ggcgcagcac gccatcgacg ccggagaggc
      961 gcgcgccgag ccgggtgatg tcgagacgcg gtttgcgctg ccgcgcagga agttacagcg
     1021 agcgatgtcg cgctatgcct cggtcgtacg ggacgctgat ggcctgcagc agctgacgcg
     1081 ggaactcgac gccgccgatc cccgcgatct cgactgccgg accagcttcg aggacgtcgc
     1141 gttgaccacc acggcgcgcg ccgtcaccgc tgctgcgttg acccgcaccg aatctcgcgg
     1201 atgccatcac cgctccgact tccccgacgc cgacccggca ttggcgcaca gccggttgct
     1261 cgaggcggcc cgctgctgat ggaattgtcc gctgacgagc tgaccgaggc tcgcagggtg
     1321 attgcgcgcg ccctggagga agacctgcgt tacgggcccg atgtcaccac gctggcgacg
     1381 gtgcccgccg atgccaccac gacggcctcg atggtgacgc gcgaccccgg cgtcatcgcc
     1441 ggggtcgaca tcgcactcct ggtcctcgac gaggtgctcg gcccggacga ataccggttg
     1501 aagtaccgcg tcgaggacgg agcccacctc gacgcgggtg gagtcgtgct ggtcgtcgaa
     1561 gcgccgacgc aggggttgtt gaccgccgag cggaccatgc tgaatctggt ttgccatctg
     1621 tcgggcatcg cgacgaccac cgctgggtgg gtcgaggccg ccgagggcac caacgcgaag
     1681 atccgcgaca cccgtaagac gctgccgggt ctgcgcgcgc tgcagaagta tgcggtgcgc
     1741 gtcggcggcg gtgtcaatca ccggatgggg ttgggcgacg cggcgctgat caaggacaac
     1801 catgtcgcgg cggccggttc ggtggtggcc gcgctgcgtg cggtccgcga ggttgcgccg
     1861 gatctgccct gcgaggtcga ggtcgattcg ctcgaacagc tcgacgacgt actggcagag
     1921 aacgtggaac tcgttctgct cgacaatttt ccggcgtggc agacgcagat cgcggtgcaa
     1981 cgccgcgatg cccgcgcacc ccagacgaaa ctcgagtcgt caggcgggct gtcgctggac
     2041 tccgcggccg aatacgcggg tacaggcgtc gactacctgg ccgtcggcgc cctcacccat
     2101 tccgttcgag tgctggatat cggtctggac ctctagccgt gaactgcgcg cgctgggctg
     2161 acgagcgttc gcacggccac cttcgcggca gtcagggcgg catcgtcacc ggcaatacgc
     2221 gacaggatca acgccccttc gatgagggcg atgacggcca gcgcagtccg ctcggctccg
     2281 ccatccgtcc agccgtcgac ccgaaggcgc tcggcgacgg cggcactcca gccggcgaac
     2341 gcgcgagcgg aaacctcgcg cacctgtgaa ctggcgttga ccgactcggt ggcgatcggc
     2401 tcgatcgggc agccgtcgcg ttggtcggag gccaactgca ctatcagcat gtcgatccag
     2461 ccgtcgacga tgtcggccac cgccgagtcg ctgtccagga accggcgcag cagccgttcg
     2521 atctcgccac ccgcggactc gacgacggcg gccgcgagtt cctgttttcc gtccgggaag
     2581 tggtgataga gcgatccggc cttgacgtct gcggtctcca agatctggtt gacgccggtg
     2641 gccgcatagc cctgccgacg gaacagcagc gccgcggtgt cgatcagcgc ggcacggctt
     2701 cgatcggggc ggggcacccc ttgacaatac tagaacggtc gctcaagaat acttgaacga
     2761 ccactaaaga agggccacgg gatgcagcag ctggtgttcg aggaggccgg gcgatacgca
     2821 tggcgggaag cagctgatcc cgagttgacc gatgcccgcc aggcgctggt gaggccgctg
     2881 atggtggccg gctgcgacct cgacgtcgcc gtcgccgagg gcgtgttgcc catgccgccc
     2941 gggcatgccg tcgggcacga gggggtggcg gaagttgtcg ccgtcggcga cgaggtcgcc
     3001 agcgtgcggg tcggcgaccg cgtcgtggtg ccgtttcaga tcaactgcgg ggactgcaat
     3061 gcgtgccgtc gcgatgtgac cgggtcctgc gcatcgcttc cgctgatggc gatgtacggg
     3121 atggctccac tcgccggggt ggacggcggc gggttcatgg ccgatctggt gatggtcccg
     3181 ttcgcggacg cgatgctgct gcgggttccc gacggtgtcg atcaggcggc gatcgcttcg
     3241 ctgtcggaca acattcccga cggctggcgc gcgatcggtc cgtaccgcgg ggagttggcc
     3301 gccctaaacg aggtcgatcg ccgcatgctt gtcgtcggca ggcggtcgat cggcttgtac
     3361 gcggcggctt tcgggtcggc gtacggggcg caggtcgact acgtcgacac cgacgaaggg
     3421 cggctcgcga tggctgagaa gctcggcgcc cgcgtccacg agcggcccag gcccgacaag
     3481 tcgtgggacc cgtatccggt gacggtgcac acgtcggcgg acccggcggt acttgccgcc
     3541 acactggccg cgacatgggc cgacggtgtc tgcaccgaca ccggcatcta ttaccggggc
     3601 gcggtcgaga tgccgctgtt ggcgatgtac acccgcggcg tccgcttcgt caccggtcgg
     3661 gtcaacgccc gggcggtgat tccggagatc ctcgaattgc tcgccgcagg ctgcgatctg
     3721 tctcctgccg tggagcgcgt ggtgccgtgg gatgacgcgc cggaagtgtg gccgacgatg
     3781 accggaaaga cgatctacgc acgggtttga cgcggcgacg gacgcctatg cggcagcaga
     3841 gccgcgacgt accagtgaca gcaccacggc ggccgcggcg aacagcccgg agaacaggcg
     3901 attcacggtc gtctgttggc ggggtgtgtg catccacttc agcagtcgcg aggcgagtgc
     3961 ggcgtagagc cccatcacca cgaggtcgac gccgaccatg gtcacgccga tggccaggta
     4021 ttgcggtagc agagcgacgt tgggcacgac gaattgcggg agcacggcga ggaagaacac
     4081 caatcccttg gggttggtcg cgttgaccag gaatccgcgg aacacaagag acagcctgcc
     4141 gccgctcgtc ggctgtccga tttgttcgcg cagatcaact gtggcactgc gccattgccg
     4201 gacggcgagg tagatcaggt agccgacgcc gatccacttg acgatgttga acgcaaggat
     4261 cgagttcgtg acgatcgcgc cgagcccaag ggccacgagc gcgagttgca gcatgaggcc
     4321 gatctcgagg ccgaggatgc tccaatatcc gcgccggaca ccgtgggtca ggccggtggc
     4381 catcgactgg atggcgccgg cgcccgggga gacggcgatc gcaatcgccg cgccgaagaa
     4441 cgtcagccag acttcccatg ccaccgcgac agtgtgtcac gcgatgtcgg cgacaagcac
     4501 tcccattttg cggacctgca cacgggccat gtacggaaga ccctcggcgc tcccgccggc
     4561 aggcacgatg cgcgggttga cgatgtgcac ggtccggctg ccgaagacca catcggcctc
     4621 gttggcggcg agcacgttct tcacccagtc ggtctttccg tgccccagcg cgatggcgag
     4681 cacgttgtcc ttgcgaaagg gtgtgacgac ggtttcatag gtcttgccgg acttgcggcc
     4741 gcggtgcctg atcgtggcca cacctggcat gatccgtgag atccgtttga ccagcgggtt
     4801 catgtacttg atctgtaggc gctcgaatgc aggcgggtag accatcggca cgccgggcgc
     4861 gttgttgggg tgatccttgg cggacatgat gtggacatta cgcatgtcat gcccgtggcc
     4921 gaagagcgcg acaagattga cgacgactcg cgctcaaagt tcgctggtgg gactcaatcg
     4981 ccgctacgat ccccgcatgt ctgagcattc ggcgccttcc aggggtttta tcgtttccgt
     5041 tttggcacta gtcgtcgcgc tggtcgccgc gggccttgca ctctgggcgt tgctgaaggc
     5101 gccggactcc caatcggcgt cgaccttcac cggcaccacg accgacgatc cgaagggcag
     5161 catctgcgag gatttcaacg tcatccgcaa cggcgtccag atcaacacga acctgcagcc
     5221 gcccggcgga cccgacgatg tcaccggctc gatggcggtg gcggccaacg cccgtctctc
     5281 gctgtacgac ggcggacagt acctactcgc gcgtctgcag ccggacacgc ctaccgatct
     5341 tgccgacgcg gttcgcaagt tcgccaacga tctgatggac atcggggcca ggtcgacctc
     5401 gggcataccg aacaccgatc ccggccaggc ggcacggttg aaggaagccg acgaggcgaa
     5461 cgccacgatc accaatttgt gcaagtgagc gcccgcctca gctgaccagc gacgcggccc
     5521 gccatgccgc ggcgatatcg atttggcctg ttctgggaca ataggtgcat gaacatggcc
     5581 cgtatcgact tgcgcggcgc cgagttgtcg gctgcccgcc tgcgatcggc gctacctcgc
     5641 ggtggcgtgg acgtcgacgc cgttgtgccg aaggtccggc cgatcgtcga tgccgtcgcg
     5701 gcgcgtggcg ccgacgccgc cctggactac ggccagtcgt tcgacggcgt gcgcccgccg
     5761 caggtgcggg ttccggctgc cgagctacag agcgccctcg atgggctacc cgccgacgtc
     5821 cgcaccgcgc tcgaggtggc aatcgagcgc acccgcgtcg tccacgccga ccagcggcgc
     5881 accgacacca cgaccacgct ggcggccggc gccacggtga ccgaacgttg ggtgcccgtc
     5941 gagcgcgtcg gcctgtacgt gcccgggggc aacgccgtct acccgtccag cgtggtgatg
     6001 aacgtcgtgc ccgcccagac ggcaggggtg gactcgctgg tcatcgcgag cccgccgcag
     6061 gcgcagttcg acggtctgcc gcatcccacc atcctggcgg ccgcccgcct gctcggtgtc
     6121 gacgaggtgt gggccgtcgg cggcgcccag ggcgttgcgc ttctggcgta cggcggaacc
     6181 gacaccgacg gaagcgaatt ggcgccggtc gacatggtca ccgggccggg caacatctac
     6241 gtcaccgccg ccaagcggat ctgccgctcg cagataggta tcgacgccga ggccgggcca
     6301 accgagatcg cgatcctcgc cgaccacacc gccgatcccg tgcatgtcgc cgctgacctg
     6361 atcagccagg cggagcatga cgaaatggcc gccagcgtgc tgatcacacc gagcgtcggg
     6421 ctggccgacg ccaccgaccg cgaactcgcc ggccagctgg aaaccaccgt gcaccggaag
     6481 cgggtgaccg cggcgctgac cggccggcaa tcggcgatcg tgctcgtcga cgacgtcgac
     6541 gcaggcatca agaccgtcaa cgcctacgcc gccgagcacc tcgagatcca gaccgccgac
     6601 ccccgtgacg tggcaggccg tatccggtcg gccggcgcga tcttcgtcgg
//
LOCUS       DASYLX010000236         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2532940,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000236 DASYLX010000000
VERSION     DASYLX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532940"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGP27_05820"
     CDS             <1..399
                     /locus_tag="VGP27_05820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7850866.1"
                     /translation="LDAYLVAAVDMFIGEVGIDPRIGDGGRGYRRRVAGLIAAGRAWA
                     RFERGQVVFKAEIGSQSPAVGQIQGVWVHPDRRGHGLGTTGTAALAAAVVRSGRIASL
                     YVNDFNTVARATYDRIGFAEVGTFATVLLD"
     gene            436..1095
                     /locus_tag="VGP27_05825"
     CDS             436..1095
                     /locus_tag="VGP27_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7850867.1"
                     /translation="MTDDAQKAERREIADTLVRALERRHELLDVIVESEDYDAAVDAI
                     AAMLGTTQSGGEAVLRLSFDRLTKVSRRRIRDELTDLNHELSFTMGDRQPAAGESLTL
                     RLFSADADRDIFAARTDDVRAAGDGSGAPAGDLDDEISAAVGRVHAEEAVWLVAIDGA
                     DKVGMVFGELANGEVNVRIWISPEFRKHGYGTAALRKSRSEMAAYFPAVPLVVRAPAA
                     G"
     gene            1234..>2567
                     /locus_tag="VGP27_05830"
     CDS             1234..>2567
                     /locus_tag="VGP27_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003906921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HEV7850868.1"
                     /translation="MATIFSSATRLASLLMIVVVSTSLNACTPKPNGPQPAAEKFLAS
                     LAKGDTGAASRLTDRPEDARAALNEAWAGLQATSLDAQVLGSKFAEDTGTVAYRYTWH
                     LPKNRVWTYDGQLNMVRNEGNWEVRWSATGLHPKLGEHQTFALRADSPRRASVNERGG
                     TDVLVPGNLYHFALDAKAAGAYLMTTATAVADALRPFDDTLDPQRLAEQASSSAGPLS
                     LITLRQSDRDQMAAVLGERPGLVITPQAELLPTDENFAPAIIGEVKKAVVDDLDGQAG
                     WRVASVNQNGVDVDVLNEVSGAPAPSITISLDRAVQNAAQNAVNGIGGKKAMIVAIKP
                     STGEILAVAQNPDADADGPTATTGLYPPGSTFKIITAGAAIERDMATPNTLLGCPGTV
                     DIGHRTIPNYGGFDLGTVPMSRAFASSCNTTFAELASQMPPRGLTQAAAQYG"
BASE COUNT          426 a          882 c          862 g          397 t
ORIGIN      
        1 ctcgacgcct atctggtggc ggccgtcgac atgttcatcg gcgaggtcgg catcgacccc
       61 cgcatcggtg acggcggacg cgggtatcgg cggcgggtcg ccgggctgat cgcggccgga
      121 cgtgcgtggg cccggttcga gcgcggtcag gtggtgttca aggcggagat cggatcgcag
      181 tcacccgccg tcggccagat tcagggcgtg tgggtgcatc ccgaccggcg cggacacggc
      241 ctcggcacga cgggcacggc cgccctcgct gctgcggtgg tgcgcagcgg gcgcatcgcc
      301 agcctctacg tcaacgactt caacaccgtg gcccgcgcga cctacgaccg catcggcttc
      361 gccgaggtcg gcaccttcgc gaccgtcttg ctggactgag ttgtccgcgc agggcggccg
      421 actacgcttc acgccatgac cgacgacgcg cagaaggccg aacgccgaga gatcgccgac
      481 acgctggtcc gcgcgctcga acggcgccac gaactcctcg acgtgatcgt ggagtcggag
      541 gattacgacg cagcggtcga cgcgatcgcg gccatgctgg gcacgacgca atccggcggc
      601 gaagcggtgc ttcgactgtc attcgaccgg ctgaccaaag tgtcgcgacg ccggatccgc
      661 gacgagctta ccgacctcaa tcacgagctg agcttcacga tgggcgaccg gcagcccgct
      721 gcgggggaga gcttgacgct gcgtctgttc tcggccgacg cggaccgcga catcttcgcc
      781 gcgcgcaccg acgatgtgcg agcggcaggc gacgggtccg gcgcgcctgc gggcgacctg
      841 gatgacgaga tcagcgccgc tgtcggcagg gtgcacgccg aagaggcggt atggctggtg
      901 gcgatcgacg gagccgacaa ggtcggcatg gtgttcggcg aactggccaa cggcgaggtg
      961 aacgtgcgca tctggatcag cccggagttc cgtaagcacg gctacggcac agccgcgctt
     1021 cggaagtcgc ggtcggagat ggcggcgtat ttccccgcag tgccgctggt cgtgcgcgcg
     1081 cccgcggccg gctgacttct gggcacccga agcgtccgcc gccgcgccga aaaccgcttt
     1141 ttggtgcgtt tcttccgcac tttctctaca aaggccggat ttcgcgtcgc gcctaagtgg
     1201 ttttttggtc tcggtttctt aacatttgcc tcaatggcaa caattttctc atctgcaaca
     1261 cgtctggcga gcctgctgat gatcgtggtg gtctccacgt ccctcaacgc ctgcacgccc
     1321 aaacccaacg ggccgcaacc cgccgccgag aagttcctcg catcgctggc caagggcgac
     1381 accggcgccg cgtcgcgact gaccgaccgt cccgaggacg cgcgggccgc gctcaacgag
     1441 gcgtgggcgg gtttgcaggc gacttcgctc gacgcgcagg tgctgggctc gaagttcgcc
     1501 gaggacaccg gcacggtggc ctatcgctat acctggcatc tgccgaagaa ccgcgtctgg
     1561 acctatgacg gccaactcaa catggtgcgc aacgaaggca actgggaggt ccgctggtcc
     1621 gcgacggggt tgcacccgaa actcggtgag caccagacct tcgccttgcg tgccgattcg
     1681 ccgcggcgcg cgtcggtcaa cgagcgcggc ggcaccgatg tgctcgtacc cggcaacctc
     1741 tatcacttcg cgctggacgc caaggccgcc ggcgcgtacc tgatgaccac cgcaaccgcg
     1801 gtggcggacg cgctgcggcc attcgacgac accctggatc cgcagcgcct cgccgaacag
     1861 gcgagttcgt cggcggggcc gctgagcctg atcaccctgc gccaatccga ccgcgaccag
     1921 atggccgccg tcctcggaga gcggcccggc ctggtcatca ccccgcaggc cgaattgctg
     1981 cccaccgacg agaatttcgc accggccatc atcggcgaag tgaagaaggc ggttgtcgac
     2041 gacctcgacg gccaggctgg ttggcgggtg gccagcgtca accagaacgg cgtcgacgtc
     2101 gacgtgctca acgaggtttc cggcgcgccc gcgccgtcga tcaccatcag cctcgaccgc
     2161 gctgtgcaga acgccgcgca gaacgcggtc aacgggatcg gtggcaagaa ggcgatgatc
     2221 gtggccataa agccgtcgac gggcgaaatc cttgccgtcg cccagaaccc cgacgccgac
     2281 gccgacgggc cgacggccac gaccggtctt tatccgccgg ggtcgacctt caagatcatc
     2341 accgcgggcg cggcgatcga gcgcgacatg gcgacgccca acacgcttct cggctgccct
     2401 ggcacggtcg acatcggcca ccggaccatc ccgaactacg gcggattcga cctcggcacg
     2461 gtgccgatgt cgcgggcctt cgcgagttcg tgcaacacga cattcgccga gctggccagc
     2521 cagatgccgc cgcgcggtct cacccaggcc gccgcgcagt acggtat
//
LOCUS       DASYLX010000237         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_558773,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000237 DASYLX010000000
VERSION     DASYLX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558773"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2559
                     /gene="ileS"
                     /locus_tag="VGP27_05835"
     CDS             <1..>2559
                     /gene="ileS"
                     /locus_tag="VGP27_05835"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV7850869.1"
                     /translation="RRFGWDTHGLPAELEVQRQLGITDKSQIEEMGIEKFNDACRASV
                     LKYTQEWRAYVTRQARWVDFDNDYKTLDLTFMESVIWAFKQLWDKGLAYEGNRVLPYC
                     WNDETPLSSHELRMDDDVYQSRQDPAITVGFRVIEGPLQDAYLLVWTTTPWTLPSNQA
                     VAVNPDVEYVVVRAGEHRFVLARARLAAYARELGEEPEILASYSGNELLGMHYLPPLP
                     YFMDAPNSFQVLPGDYVTTDDGTGIVHTSPAYGEEDMAIAQAADIVPVTPVDAGGRFD
                     QTVPDYAGQHIFDANPQIIRDLKNQSGPAAQNRPVLLRHETHEHSYPHCWRCRNPLIY
                     RAVSSWFVKVTEFRDRMVQLNEQITWYPEHVKDGQFGKWLSNARDWSISRNRYWGSPI
                     PVWTSDDPAYPRIDVYGSLDELERDFGVRPDNLHRPYIDELTRPNPDDPTGKSTMRRI
                     PDVFDVWFDSGSMPYAQVHYPFDNAGWFESHFPADFIVEYIGQTRGWFYMMHVLATAL
                     FDRPAFKTCVSHGIVLGNDGQKMSKSLRNYPDVSEVFDRDGSDAMRWFLMASPILRGG
                     NLIVTEQGIREGVRQVQLPIWNAYTFLALYAPKKGTWRTNSTHVLDRYILAKLAQLRD
                     DLTTSMDVCDISGACDELRQFTEALTNWYVRRSRSRFWEEDPDAIDTLHTVLEVLGRL
                     AAPLLPLTTEVIWRGITGERSVHLTDWPEEGDLPHDPDLVATMDQVREVASAASSLRK
                     AKKLRVRLPLPKLTVAVENPQRLEPFRELIADELNVKEVELTDDIPEFGRFELTVNAR
                     AAGPRIGKDVQAAIKAVKAGEGVVNSDGTLTAGPAVLLPEEFSSRLVAADPEFTA"
BASE COUNT          477 a          812 c          827 g          443 t
ORIGIN      
        1 cgccgcttcg gctgggacac ccacgggctg cccgccgagc tggaagtgca gcggcagctc
       61 ggcatcaccg acaaatcgca gatcgaagag atgggcatcg agaagttcaa cgatgcctgt
      121 cgcgcgtcgg tgctcaagta cacgcaggag tggcgcgcat acgtgacgag gcaggcgcgt
      181 tgggtcgact tcgacaacga ctacaagaca ctggatctca cgttcatgga gtcggtgatc
      241 tgggcgttca agcagttgtg ggacaaaggt cttgcctacg aaggcaaccg ggtgctgccg
      301 tactgctgga acgacgagac cccgctgtcc agccacgaac tgcggatgga cgacgacgtc
      361 tatcagagcc gtcaggatcc ggccatcacg gtcggattcc gagtcatcga aggcccgctg
      421 caggatgcgt acctgttggt gtggacgacc acgccgtgga ccttgccgtc caaccaggcg
      481 gtcgcggtca atccggatgt cgagtatgtg gtcgtacgcg cgggggagca ccggttcgtg
      541 ctggcgaggg cgcgcctggc cgcctacgca cgcgaactgg gcgaggaacc cgagatcctg
      601 gccagctatt cgggcaacga acttctcggc atgcactacc tgccgccgct gccgtatttc
      661 atggacgcgc ccaacagttt tcaggtgctg ccgggcgatt acgtcaccac cgacgacggc
      721 accggcatcg tgcacacgtc acccgcctac ggcgaggagg acatggccat cgcacaggcg
      781 gccgacatcg tccccgtcac gccggtggac gcggggggcc ggttcgacca gactgtgccc
      841 gactacgcgg gccagcacat cttcgacgcc aacccgcaga tcatccgcga tctcaagaac
      901 cagagcggtc ccgccgcgca gaaccggccg gtgctgctgc ggcacgaaac ccacgaacac
      961 tcctacccgc actgctggcg gtgccggaac ccgttgatct accgggccgt ttcgtcctgg
     1021 ttcgtcaagg tcaccgaatt ccgcgaccgc atggtgcagc tcaacgagca gatcacctgg
     1081 taccccgaac acgtcaagga cggccagttc ggcaagtggc tgtccaatgc ccgtgactgg
     1141 tcgatctctc ggaatcgcta ctggggcagt cctattccag tgtggacatc cgacgatccg
     1201 gcttatccgc gcatcgacgt gtacggcagc ctcgacgagt tggagcggga cttcggtgtc
     1261 cggccggata acctgcaccg gccctacatc gacgagttga cgcggcccaa tcccgacgac
     1321 ccgaccggca agtccacgat gcggcgcatt cccgacgtgt tcgacgtctg gttcgactcc
     1381 ggatcgatgc cctacgccca agtgcactac ccgttcgaca acgccggctg gttcgagtcg
     1441 catttcccgg cggacttcat cgtggagtac atcggacaga cgcgcggctg gttctacatg
     1501 atgcacgtcc tggccaccgc gttgttcgat cgcccggcgt tcaaaacctg tgtgtcgcac
     1561 ggcatcgtgc tgggtaacga cggccagaag atgagcaagt cgctgcgtaa ctatccggac
     1621 gtctccgagg tcttcgaccg ggacggttcc gacgccatgc gctggttcct catggcgtca
     1681 cccatccttc gcggcggcaa cctgatcgtc accgaacagg gcatccgtga gggtgttcgg
     1741 caggtgcagc tgccgatctg gaacgcctac acatttctgg cgctgtacgc gccgaagaag
     1801 ggcacctggc ggacgaactc gacgcatgtc ctggatcgct acatcctggc caagctggcg
     1861 cagctgcgcg atgacctgac cacgtcgatg gatgtctgcg acatctcagg agcctgcgac
     1921 gaattgcgcc agttcaccga ggcgctgacg aattggtatg tgcgacggtc gcgttcgcga
     1981 ttctgggaag aggatcccga cgctatcgac acactgcaca ccgtcttgga ggtgctgggc
     2041 aggctggccg cgccgctgct tccactgacg accgaggtga tctggcgcgg catcaccggc
     2101 gagcgttcgg tgcatctcac cgattggccg gaagaaggcg acctgccgca cgatcccgac
     2161 ctggtcgcaa cgatggatca ggtgcgcgag gtggcctcgg cggcgtcgtc gctgcgcaag
     2221 gcgaagaagt tgcgggtgcg gttgccgctg cccaagctga ctgtcgctgt ggagaacccg
     2281 cagcgcctgg aaccgtttcg ggaactgatc gccgatgagc tgaatgtcaa ggaagtggag
     2341 ctgaccgacg acattccgga gttcggccgg ttcgagctga cggtcaacgc gcgcgcagcg
     2401 gggccgcgca tcggcaagga cgtgcaggcc gcgatcaagg ccgtgaaggc cggcgagggc
     2461 gtcgtcaatt cggacggcac gctgactgcg ggaccagcgg tactgctgcc cgaagagttc
     2521 agctcgcgac tggtggccgc cgatccggag ttcaccgcg
//
LOCUS       DASYLX010000238         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2642526,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000238 DASYLX010000000
VERSION     DASYLX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642526"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VGP27_05840"
     CDS             complement(<1..669)
                     /locus_tag="VGP27_05840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV7850870.1"
                     /translation="MPRKQFRPDIEGLRAVAVIAVVLFHAGVPGVGGGFVGVDVFFVV
                     SGFLITGLLWREASNTGSVRLARFYGARARRLLPAAVTVLVATCGASAVLLPPLQARS
                     VIGDGIASALYVGNYRFAIQGTDYLAADAPPSPMQHYWSLGVEEQFYLLWPALIIGTA
                     WLLARVARRFGGTATRSAAPYVVLLGLLAAMSFVVSLVWTDTWPSWAFFSLPTRAWEL
                     AVGGL"
     gene            complement(789..1640)
                     /locus_tag="VGP27_05845"
     CDS             complement(789..1640)
                     /locus_tag="VGP27_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850871.1"
                     /translation="MPPMTRRMLLRLGAGFAGASALDPLWQLGLATASGAPPVDQPSP
                     SSAKTTGTYVTGSFVSAARGGVETKWAIALPPGRYSRPLRPVIALHGRDMNADGVMGM
                     GVEAGLAELAKAGYPPFAVVAVDGGNGWWHRRVSGEDSGTMVLTELLPMLAAKGLDTS
                     RVSFIGWSMGGYGALLLGGALGPARTAAICAVAPALFTDYGLALQEGAFDGPADWFAY
                     SVYGTPALSEIPLRIDCGAEDRFYNATKQFVASLRRPPSGVSFIGGHDVAAWREKLPR
                     ELAWLAT"
     gene            complement(1648..>2419)
                     /locus_tag="VGP27_05850"
     CDS             complement(1648..>2419)
                     /locus_tag="VGP27_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEV7850872.1"
                     /translation="QARCERDGGARTGEGLWQASEIYAPLEAWQALGQPTIAINANFF
                     DVRGQQGGSWRDTKCSSPLGAYVDNTRGQGRANTAVTGTLAYAGKQGLSGGGEHWSSL
                     ATMILPVSARPSVVMPKAPNDYDSASPEIAKLLDQNARFVAVAGIGLLAPGDTGQLND
                     GGPSAARTAIAYNRTNDQLYVFQGGSYTPDQIQDLFRGLGSDTAVLLDGGGSSAIVLR
                     RDTGGMWSGAGVPKGSCDTQQVLCDSRERALPSWLAFA"
BASE COUNT          449 a          866 c          762 g          342 t
ORIGIN      
        1 cagtccgccg accgccaact cccacgcccg cgttggcagc gagaagaatg cccacgacgg
       61 ccaggtgtcg gtccacacca gcgacaccac gaacgacatc gccgccagca agccgagtag
      121 cacgacatac ggcgccgcag aacgcgttgc cgttccgccg aaccgtcgag ccacccgcgc
      181 cagaagccat gccgtaccga tgatcaacgc cggccacagc agatagaact gctcctccac
      241 gcccagcgac cagtagtgct gcatcggcga gggcggcgca tccgccgcca gataatccgt
      301 tccctgaatc gcgaaccggt agttgccgac atacagtgcg ctcgcgatgc catcgccgat
      361 gacgctgcgc gcctgcagcg gcggcaacag gaccgccgag gccccacacg tcgcaacaag
      421 caccgtgacc gcggccggaa gcaggcgtcg cgcccgtgcg ccgtagaacc gggccagccg
      481 caccgacccg gtattggatg cctcacgcca tagcaggccg gtaatcagga atccggagac
      541 gacgaagaac acgtcgaccc cgacgaaccc gccgccgacg ccgggtacac cggcatggaa
      601 cagtacgacg gcaatgacgg caaccgcccg cagcccttcg atgtcgggcc ggaactgctt
      661 ccgcggcaga gccgtccgcc cgctcgcagg agaaccacgt cggtaggcgg ggcggggccc
      721 agtcgtaact ctgttcactc gagcaacgat cgtctcatgg gacccaggcg tatcccggga
      781 tcaacgcgct aggtagccag ccacgccagc tctcgcggta gcttttcgcg ccacgccgcc
      841 acatcgtggc caccgatgaa cgatactccc gatggtggtc tgcgcagcga cgcgacgaac
      901 tgtttggtgg cgttgtaaaa ccggtcttcg gcaccgcagt cgatgcgcag cggaatctcg
      961 gagagtgcgg gggtgccgta caccgagtac gcgaaccaat cagccggacc atcgaaggcg
     1021 ccttcttgaa gcgccaagcc gtagtcagtg aagagcgccg gtgcgacggc gcagatcgcg
     1081 gccgttcgcg ccggcccgag ggcgccgccg agcaacaagg cgccatagcc gcccatcgac
     1141 cacccgatga aactcaccct ggaagtgtcc aggcccttgg ctgcaagcat cggcagcagt
     1201 tcggtgagca ccatcgttcc cgagtcctca ccgctgactc gtctgtgcca ccacccgttt
     1261 ccgccgtcca cagccaccac cgcgaaaggc ggatagcccg ccttcgccaa ctcggccaga
     1321 cccgcttcga cgcccatacc catgacaccg tcggcgttca tatcacgccc gtgcaacgcg
     1381 atgacgggtc gcaacggccg actgtagcgg cccggcggca gggcgatcgc ccacttggtc
     1441 tcgacgccgc cgcgtgccgc ggagacaaac gaaccggtga catacgtccc cgtcgtcttc
     1501 gccgacgagg gcgacggctg atcgaccggc ggagcaccgg acgcggtggc aaggcccaac
     1561 tgccacaacg gatccaatgc acttgcgccc gcgaatcctg cgcccagccg caggagcatg
     1621 cggcgcgtca tcggtggcat cggcgtgcta ggcgaaggcg agccagctgg gaagcgcgcg
     1681 ctcacgcgaa tcgcagagca cctgctgtgt gtcgcaactg ccctttggaa cgccggcgcc
     1741 gctccacatt ccgccggtgt cacggcgcaa cacaatggcc gacgagccgc ccccgtccag
     1801 aagcacagcg gtgtcggagc ccaagccgcg gaacaggtcc tgaatctggt cgggtgtata
     1861 gctgccgccc tggaacacgt acagctgatc gttggtccgg ttgtaggcga tcgccgttcg
     1921 cgctgcactg gggccgccgt cgttcagctg accggtgtcc cccggcgcaa gcagaccgat
     1981 tcccgcgacc gcgacgaatc gcgcattctg gtcgagcagc ttcgcgattt ccggggaggc
     2041 cgagtcatag tcgttggggg ccttgggcat gacgacggat ggcctcgcgc tgaccggcag
     2101 gatcatcgtg gccagcgacg accagtgctc gccgccgccc gacagaccct gcttgcccgc
     2161 gtaggcgagg gtgccggtga cagcggtgtt ggcccggccc tgtccccgag tgttgtcgac
     2221 gtacgcgcca agcggggaac tgcatttggt gtcgcgccac gacccgccct gctgaccgcg
     2281 cacgtcgaag aagttcgcgt tgatcgcgat cgtcggctgc ccgagggcct gccacgcctc
     2341 cagcggcgca tagatttcag acgcctgcca caacccttcg ccggtgcgtg cgccgccgtc
     2401 gcgctcgcag cgagcctgg
//
LOCUS       DASYLX010000239         6710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2971764,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000239 DASYLX010000000
VERSION     DASYLX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971764"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VGP27_05855"
     CDS             complement(<1..1242)
                     /locus_tag="VGP27_05855"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891205.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEV7850873.1"
                     /translation="MFSHDLPSFADRHIGPDADAVATMLKAIGVGSLDELADKALPAG
                     ILDALSTDGIAPGLDHLPQAASEEQALAELRALADANTVAVSMIGQGYYDTLTPPVLR
                     RNILENPAWYTAYTPYQPEISQGRLEALLNFQTMVADLTGLEVANASMLDEGTAAAEA
                     MTLMHRAVRGTSNRMAVDSDLYKQTAAVLATRAEPLGIEIVTADLRDGLPDGEFFGVI
                     TQLPGAGGQVVDWTDLVSQAHERGALVAIGADLLALTLISPPGEIGADVAFGSTQRFG
                     VPMGFGGPHAGYLAVHAKHARQLPGRLVGVSVDADGSPAYRLSLQTREQHIRRDKATS
                     NICTAQVLLAVIAAMYASYHGAEGLTGIARRVHDRARTLAAGLSAAGVEVVHDRFFDT
                     VLAKVSGRATQVRDEAKSRGVN"
     regulatory      complement(1330..1427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1478..2101)
                     /locus_tag="VGP27_05860"
     CDS             complement(1478..2101)
                     /locus_tag="VGP27_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7850874.1"
                     /translation="MEDTPRQEQLDLSSNDGASQPSAPVASEPVQAGLFPDDSVPDEL
                     VGYRGPSACQIAGITYRQLDYWARTSLVVPSIRSAAGSGSQRLYSFKDILVLKIVKRL
                     LDTGISLHNIRVAVDHLRQRGVRDLANITLFSDGTTVYECTSAEEVVDLLQGGQGVFG
                     IAVSGAMRELTGAIADFPGERADGGESISAPEDELAHRRKHRDRKIG"
     gene            complement(2317..2811)
                     /locus_tag="VGP27_05865"
     CDS             complement(2317..2811)
                     /locus_tag="VGP27_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV7850875.1"
                     /translation="MGEVRVVGIRVEQPQNQPVLLLRESNGDRYLPIWIGQSEAAAIA
                     LEQQGVEPARPLTHDLIRDLIAALGHSLKEVRIVDLQEGTFYADLIFDRDIKVSARPS
                     DSVAIALRVGVPIYVEEAVLAEAGLLIPDENDDEATGAVREDEVEKFKEFLDSVSPDD
                     FKAT"
     gene            complement(2860..3594)
                     /locus_tag="VGP27_05870"
     CDS             complement(2860..3594)
                     /locus_tag="VGP27_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7850876.1"
                     /translation="MTQPGVQSPLTGMSIGAVLDSLRPDFPDVTISKIRFLEAEGLVT
                     PQRSASGYRRFTAYDCARLRFVLTAQRDQYLPLKVIKAQLDAQPDGELPHTGSPYGVP
                     RLVPVPSEPAALDAAVAPTQVRMSREDLVARSGVDDTVLTALIKEGVITTGAAGFFDE
                     HSVVIAQCARALADYGVEPRHLRAFRSAADRQSDLIAQIAGPVVKAGKAGARDRADDL
                     AREVAALAITLHTSLIKSAVRDVLDR"
     gene            complement(3620..4102)
                     /gene="garA"
                     /locus_tag="VGP27_05875"
     CDS             complement(3620..4102)
                     /gene="garA"
                     /locus_tag="VGP27_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen accumulation regulator GarA"
                     /protein_id="HEV7850877.1"
                     /translation="MTDKDQNSGADQTSDELTVETTSVFRADFLNELDAPASAGSEGA
                     VSGVEGLPVGSALLVVKRGPNAGSRFLLDQPTTSAGRHPDSDIFLDDVTVSRRHAEFR
                     LENSEFQVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKTDDDSGPGT
                     "
     gene            complement(4280..4675)
                     /gene="gcvH"
                     /locus_tag="VGP27_05880"
     CDS             complement(4280..4675)
                     /gene="gcvH"
                     /locus_tag="VGP27_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV7850878.1"
                     /translation="MSEIPPDLYYTSEHEWVQRIDDETVRVGITDFAQSSLGDVVFVQ
                     LPDIGADVTAGESFGEVESTKSVSDLYAPVTAKVVAVNGDLETSPQLVNSDPYGAGWL
                     VELQADAETLAGQLDDLLDADAYQALVTE"
     gene            complement(4700..5539)
                     /locus_tag="VGP27_05885"
     CDS             complement(4700..5539)
                     /locus_tag="VGP27_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEV7850879.1"
                     /translation="MSGDTPDSAGAHEAEHAGRHELPPDEPVQRIGDLQPAGVKGVMR
                     RGRSQLVFGALGALLCVLLGVAIVTQVRQNESGDSLETARPADLLVLLDSLQQREAVL
                     NTEVADLQRTLASLQASGSSDQAAIENAQARLAALSILIGTVPATGPGVTLTIEDAAP
                     GVAPETMLDVVNELRNSGAEAMEVQGGAGDPKISVRVGVDTWVVGRPGALEIDGQTLN
                     PPYTVVAIGDPPTLAAAMNIPGGAMDSVERVGGTMVVQQSDRVDVTALRQPKARQYAQ
                     PVK"
     gene            complement(5710..6042)
                     /locus_tag="VGP27_05890"
     CDS             complement(5710..6042)
                     /locus_tag="VGP27_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HEV7850880.1"
                     /translation="MIGIAALVIGIVLGLVFHPSVPHFVEPYLPIAVVAALDAVFGGL
                     RAYLERIFDSKVFVVSFVFNVLVAALIVYVGDQLGVGTQLSTAIIVVLGIRIFGNAAA
                     LRRRLFGA"
     gene            complement(6039..>6710)
                     /locus_tag="VGP27_05895"
     CDS             complement(6039..>6710)
                     /locus_tag="VGP27_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEV7850881.1"
                     /translation="AVAQAQATAPGVRESQQVLSGSVRSAEEATKSATARRDALAAEV
                     DTERRSRLEGDERGRRLLGGLDGANFSAAATAVTGPGLTVTVTDPGVSKDLSDVSKER
                     VEGSRQVILDRDLQLVVNSLWVSGAEAISVGGVRIGPNVTVRQAGGGILVDNQPISSP
                     YVILAVGPPHGMGDIFDRSPGLQRLRLLEASYGVVVTVSATDNVTVPAGSVRDVNFAK
                     EIGDR"
BASE COUNT         1055 a         2314 c         2184 g         1157 t
ORIGIN      
        1 gttgacgccg cgcgacttgg cctcgtcgcg aacctgtgtg gcgcgaccgg acaccttcgc
       61 cagcacagtg tcgaagaagc ggtcgtgcac cacctcgacg cctgccgccg acagcccggc
      121 cgccagggtg cgggcacggt cgtgcacccg ccgggcgatg ccggtcagcc cctcggcgcc
      181 gtgatagctc gcgtacatcg cggcgatcac cgccagcagc acctgcgcgg tgcagatatt
      241 gctggtggcc ttgtcgcggc gaatgtgctg ttcgcgggtc tgcagggaga gccggtaggc
      301 cggtgagccg tcggcgtcca ccgagacccc gacaagtcgg cccggcagtt gcctcgcatg
      361 cttggcgtgc acggccagat agccggcgtg cggtccgccg aaacccattg gcacaccgaa
      421 gcgttgggtg ctgccgaacg ccacgtcggc accgatctca cccggcgggc tgatcagcgt
      481 cagcgcgagc agatcggccc cgatcgccac cagcgcgccg cgctcatggg cctggctcac
      541 cagatcggtc cagtcgacca cttggccacc ggcgccaggc agttgggtga ttaccccgaa
      601 gaactcgccg tcgggcaggc cgtcgcgcag gtcggcggtg acgatctcga tgccgagcgg
      661 ctcggccctg gtggccagca cggcggcggt ctgcttgtac agatcgctgt cgacggccat
      721 ccggttcgac gtgccgcgca ctgcgcggtg catcagggtc atcgcctcag cggcggcggt
      781 gccctcgtcg agcatcgatg cgttggccac ctcgaggccg gtgagatcgg cgaccatggt
      841 ctggaagttc agcagtgcct ccagcctgcc ctggctgatt tccggctgat acggcgtgta
      901 cgccgtgtac caggccgggt tctcgaggat gtttcgccgc agcaccggcg gagtcagcgt
      961 gtcgtaatac ccctggccga tcatcgagac agcgacggtg ttggcgtcgg cgagtgcgcg
     1021 caactcggcc agcgcctgct cttcactcgc ggcctgaggc aggtgatcga ggccgggcgc
     1081 gatgccatcg gtggacaacg cgtcgagaat gcctgcggga agcgccttgt cggcgagttc
     1141 gtcgagcgag cccacaccga tggccttgag catggtggcg acggcatcgg cgtccggccc
     1201 gatgtgccgg tccgcgaaac tcggtagatc gtgggagaac atccatctcc tcaacgcttg
     1261 gggcaggaaa cccgctaggt ttcccctccc tcagtcgtcg acccggacac gggcgctcca
     1321 gaggcatcgg ggccctgcgc agtccgggtg cctgagaggt tgacggagag gtgttgctcc
     1381 ttcggcgtcc gtgactggcg atcacggaac tctcccgcgt aagggcgatg cgcagccgat
     1441 tctaccgaca ctcggtcgga gcggcacgtt gccgggttca gccgatcttg cggtcgcggt
     1501 gcttgcgccg gtgagccaac tcgtcctcgg gcgcactgat cgactcgcca ccgtcggccc
     1561 gctcgccggg gaaatcggcg atggcgccgg tcagctcccg catcgcgccg gagactgcaa
     1621 tgccgaacac accctgaccg ccctgcagca ggtcgaccac ttcctccgcc gacgtgcact
     1681 catagacggt ggtcccgtcg gagaacagcg tgatgttggc cagatcacga acaccgcgct
     1741 ggcgcaggtg gtcgacggcg acgcgaatgt tgtgcagcga gatgccggtg tccagcagtc
     1801 gcttgacgat cttgaggaca aggatgtctt tgaacgaata cagccgttgg ctgccggatc
     1861 cggcggcgct gcggatcgac ggcacgacca gtgaggtcct ggcccagtaa tcgagctgtc
     1921 ggtaggtgat gcccgcgatc tggcaggcgc tcggcccccg atagccgacg agctcgtcgg
     1981 gcaccgagtc atcgggaaac aggccggcct gtacgggttc gctggcgacc ggtgctgacg
     2041 gctgggatgc accatcgttc gacgaaaggt ccagctgctc ttgacgtggc gtgtcttcca
     2101 ctctcgaatc ctctcaccga tcgaactcgc gtctgtgacc tgcaccgcgg cacgtttgcc
     2161 caagctcgaa tgtgtcgagc atacgctccc cgacgaaccc tgactgcccg tcgcggccat
     2221 caaagtatgg ctgcccccat caccgttctg ggaaacccgc gccgcgtgtc gagcccgaaa
     2281 gcgccagttg agacgcatcc gtgatgttcg tacggctcag gtggccttga agtcgtccgg
     2341 cgacacgctg tcgaggaact ccttgaactt ctcgacctcg tcctcgcgca ccgcgcctgt
     2401 ggcctcgtcg tcgttctcgt cggggatcag cagtcccgcc tcggcgagca ccgcctcctc
     2461 gacgtagatc ggcacgccca cccgcagcgc gatggccacc gagtcggacg gcctcgccga
     2521 caccttgatg tcgcgatcga agatcaggtc ggcgtagaag gtgccctcct ggaggtcgac
     2581 gatgcgtacc tctttgagcg aatgaccaag cgccgcaatg agatctcgga tcaggtcgtg
     2641 ggtcagcggg cgggcaggtt cgacgccctg ctgctcgaga gcgatcgccg ccgcctccga
     2701 ctggccgatc cagatcggca gatagcggtc accattggac tcgcgcagca gcaacaccgg
     2761 ctggttctgg ggctgctcca cgcgaatgcc gaccacacga acctcaccca tctgtgtctg
     2821 ccctccgcac cgctgaagcc aatgcgacaa gtctaatcct cacctgtcga gtacgtcgcg
     2881 cacggccgac ttgatcaacg acgtgtgcaa cgtgatcgcc agcgcggcca cctcgcgggc
     2941 cagatcgtcg gcgcgatccc gcgcacccgc cttgcccgcc ttgaccaccg gccccgcgat
     3001 ctgagcgatc agatcggact gccggtcggc cgccgaccgg aacgccctga gatgacgagg
     3061 ctcgaccccg tagtcggcga gcgcacgcgc gcactgggcg atgaccacgg aatgctcgtc
     3121 gaagaatccg gccgctcccg tggtgatcac accctccttg atcaaggcgg tcaggacggt
     3181 gtcgtccaca cccgaacgcg caaccaggtc ttcgcggctc atccgcacct gcgtcggcgc
     3241 cacggccgcg tcgagtgcgg caggctcgct gggcacaggc accaaacgcg gtacgccgta
     3301 gggggatccg gtgtggggta gttcaccgtc gggctgagcg tccagctggg ccttgatgac
     3361 cttcagcggt aggtactggt cgcgctgcgc ggtcaggacg aagcgcagcc gggcacagtc
     3421 gtaggcggtg aaccgccgat agcccgacgc cgaccgctgc ggcgtcacca gtccctcggc
     3481 ctccagaaaa cggatcttgg agatcgtcac gtccgggaag tccggtcgca gcgaatcgag
     3541 taccgctccg atcgacatcc cggtcagcgg agattggacg ccgggctgtg tcatcttctc
     3601 ttcgcgcgag cgctcatcgt tacgtgcccg gaccgctgtc gtcgtcggtc ttgggtccgg
     3661 tgaggaacac caggcggaac ttgccgatct ggacctcgtc gccgttggcc agcaccgccg
     3721 agtcgaccgg ctccctgttg acgtaggtgc cgttgaggct gcccacgtca acgacctgga
     3781 actcactgtt ctccagccgg aactccgcgt ggcgacgact cacggtgacg tcgtcgagga
     3841 agatatcgct gtcgggatgt cgcccggcgg acgtggtcgg ctggtcgagc aggaagcgtg
     3901 atcctgcgtt ggggccgcgc ttgacgacaa gcaacgccga tccgaccggc aggccctcga
     3961 cccccgagac cgccccctcg gaccccgccg atgccggggc gtccagttcg ttgaggaagt
     4021 cggcgcggaa tacggatgtc gtctccacgg tcaactcgtc agacgtctgg tcagccccag
     4081 aattctgatc cttgtccgtc acccgctgct cctcactggc tgctgtggcg atatctggcc
     4141 gggcccgctg tgggtcccct gtccatctgt cattcgtttc acgtcgaccg taccgcgctg
     4201 cggtcgacgt tgggtccacc cccgccggat caaacgtgaa gacctctggg cgtcagacgc
     4261 tggcaaccct aacaagatct cattcggtca ccagcgcctg gtaggcgtcc gcgtcgagca
     4321 ggtcgtcgag ctgtcccgcc agcgtctcgg cgtcggcctg caactcgacg agccagcccg
     4381 cgccgtaggg atcggagttg accagctggg ggctggtctc cagatcgccg ttgacggcaa
     4441 cgactttcgc ggtcacgggt gcatagaggt cggacaccga cttcgtcgac tccacctcgc
     4501 cgaacgactc ccccgcggtc acgtcggcgc cgatgtcggg cagctggacg aacaccacat
     4561 cgccgagtga cgactgggca aaatcggtga tgccgaccct tacggtctcg tcatcgatgc
     4621 gctgaaccca ctcgtgttcg gatgtgtagt acaagtcggg cgggatttcg ctcactgcgc
     4681 tcctctttgc gggtcgggct cacttcacgg gctgagcgta ttggcgggct ttcggttgcc
     4741 gcaaggcggt cacatcgacc cggtcggatt gttgtaccac catcgtcccg cccactcgtt
     4801 cgacactgtc catcgccccg ccgggaatgt tcatcgcggc cgcgagtgtc ggcggatccc
     4861 caatcgccac aacggtatac ggcgggttga gggtttggcc gtcgatctcg agcgcgccag
     4921 gccggccgac cacccaggta tccaccccga cccggacgga gatcttcggg tcgcccgctc
     4981 cgccctggac ctccatcgcc tcggcgccgg agttccgcag ctcgttgacc acatcgagca
     5041 tggtttccgg ggcgaccccc ggggcggcgt cctcgatggt cagcgtgacc cccggacctg
     5101 tcgcaggcac ggtgccgatc aagatcgaaa gcgccgccag ccgggcctga gcgttctcga
     5161 tcgcggcctg atcactgctg cccgacgcct gcagcgacgc cagcgtccgc tggaggtcgg
     5221 ctacctctgt gttcaggacg gcctcccgct gctgcaggga atccagcagc accaggaggt
     5281 cggccggacg tgcggtctcc agcgagtcgc cggactcgtt ctgccgcacc tgggtgacga
     5341 tcgccacccc gagcagcacg cacagcaggg cgccaagcgc cccgaacacc agctgcgacc
     5401 gcccgcgacg catcacaccc ttgactcccg cgggctgaag gtcgccgatc cgctgcacgg
     5461 gttcgtcggg cggcagttcg tgtcggcctg cgtgttcggc ttcgtgtgcg cccgcgctgt
     5521 cgggggtgtc gccgctcatg cctcggctcc cgctcgtccc ctgccctgcg ggcgtgggcc
     5581 caccggtcct ctgccgtcgc ctcgactctc ggtcgctccg gtcctcgctg cgctgcgatc
     5641 ctcactcccg atcgtcttcg ggtgctgcgc tgcgatcctc actcgcgctc gccttcgggt
     5701 gctcatgcgt caggcgccga agagtctgcg gcgcagggcc gccgcgttgc cgaagatgcg
     5761 gatgccaagt acgacgatga tcgcggtcga cagttgtgtg ccgacgccga gctggtcgcc
     5821 gacatagacg atgagcgcgg cgacaaggac gttgaacacg aacgacacca cgaagacctt
     5881 ggagtcgaag atccgctcta ggtatgcccg cagcccgccg aacaccgcgt cgagcgcggc
     5941 gaccaccgca atcggcagat agggttcgac gaaatggggc acgctgggat ggaagaccag
     6001 gccgaggacg atgccgatga caagtgcggc gattccgatc atctgtctcc gatttccttg
     6061 gcgaagttga catctcgcac cgaacccgcg ggcacggtca cgttgtcggt tgcgctgacg
     6121 gtgacgacta cgccatagct ggcctccaac aggcgaagcc gttgcaggcc ggggctgcgg
     6181 tcgaagatgt cccccatgcc gtgcggtggg ccgaccgcca gaatcacgta ggggctgctg
     6241 atcggttgat tgtcgaccag gatgcccccg cccgcctgcc ggacggtcac attgggtccg
     6301 attcgcacgc cgcctaccga gatcgcttcg gcgccgctga cccacagcga gttgaccacc
     6361 agttgcaggt cccgatccag gatgacctgc ctgctgcctt cgactcgttc cttcgagacg
     6421 tcgctcaggt ctttcgagac accgggatcg gtgaccgtca ccgtcagacc gggaccggtc
     6481 accgcggtgg ccgccgccga gaagttggcg ccgtcgaggc cccccagcag tcgcctgcca
     6541 cgctcgtcgc cctccaggcg gctgcgtcgc tcggtgtcga cctcggcggc cagcgcgtcg
     6601 cggcgggcgg tcgccgactt cgtcgcctcc tcggcggacc gcacgctgcc cgacagcacc
     6661 tgctggctct cccgcacacc cggcgcggtg gcctgcgcct gcgcgacggc
//
LOCUS       DASYLX010000240         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2314436,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000240 DASYLX010000000
VERSION     DASYLX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314436"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1135
                     /locus_tag="VGP27_05900"
     CDS             323..1135
                     /locus_tag="VGP27_05900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7850882.1"
                     /translation="MSNQYAWGNTEDERQRLASQGDALRPATRRLFQAAGIGPGMHVL
                     DCGSGAGDVSIIAAELVTASGRVLGIDRDAGSVEAANRRVRGIGLSHAHFEIADISSP
                     PDGPFDAIVGRLVLMYQPDVEPVLRALADRLDQGGVMAFMEYEHHPPNQVLMWPRSPS
                     VDQLVAWMDATFEVLNHQAHMGTRLPSLLRSVGLEPQPPYEVTGAVYTGATVLEHETT
                     LVQNLSPLLTAHGIASEHETDIGAFAERVGHDLGPEPVLVSGPHLSVWARKP"
     gene            complement(1161..>1715)
                     /locus_tag="VGP27_05905"
     CDS             complement(1161..>1715)
                     /locus_tag="VGP27_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7850883.1"
                     /translation="VVEKISAAAAGMPVGLPDDPGAMIGPLISEKQRERVEGYIRKGV
                     EEGARLVAGGGRPEGLDSGWFVQPTVFADVDNSMTIAQEEIFGPVLAVIPYDTEDDAV
                     RIANDSPYGLAGSVFTSDNEKAMKIAAKIRTGTYAVNMYAFDPGAPFGGYKNSGIGRE
                     NGPEGIEQYTQAKSVLLPFGYKPE"
BASE COUNT          300 a          558 c          525 g          332 t
ORIGIN      
        1 cagcgcaact gcagtcggga caatcgaata gttgccgacg acgccacacc accggttgaa
       61 cgcggtcagg tgctgagcac gatgttcagc tgcgaattcc ccgtaggagg caaccatgtc
      121 tcgagcatcc caccaaaaaa atcaaaacgg gaccgaaacg catggctact cccgtttgta
      181 gccgaacggc gcgccgggat cgaacgcgta cctgttgacc gcatgggtgc cggtgcggat
      241 cttcattgcc ttctcgtttc tcctggcgag cgggttgttt tttcagccaa tcgcgacgaa
      301 tccgtgtgca accctcgtca ttgtgagcaa ccaatacgct tggggaaaca cagaggacga
      361 acgccaacgg ctcgcaagcc aaggcgacgc gctgcgtccc gcgacgagga gactgttcca
      421 agcagctggc atcggtcccg ggatgcatgt gcttgattgt gggtcaggcg ctggcgatgt
      481 ctccatcatt gccgccgagc tggtgaccgc ctccggccga gttctgggca tcgaccgtga
      541 cgccggcagc gtcgaggccg cgaaccgccg tgtcagggga atcggcctgt cgcacgccca
      601 ctttgagata gctgatatct cctcccctcc cgacgggccg ttcgacgcga tcgtcgggcg
      661 gttggtgctg atgtaccagc ccgacgtcga gccggtgctg cgcgccctgg ctgatcgtct
      721 cgaccagggc ggtgtcatgg cattcatgga gtacgaacac catccaccca atcaagtgtt
      781 gatgtggccg cgcagcccat cggtggacca attggtggca tggatggacg ccaccttcga
      841 agtgctcaat catcaagcac acatgggtac gcgcttgccg tcgctgttgc ggtcggtcgg
      901 cctggaaccg caacccccct acgaggtgac tggtgcggtg tataccggcg cgaccgtgct
      961 ggaacacgag accacattgg tgcagaacct gtcaccgctg ctgaccgctc acggcatcgc
     1021 gagcgagcat gagacggaca tcggcgcctt cgccgaacgt gtcggacacg acctaggccc
     1081 cgagccggtg ctggtttcag ggccgcacct ttcggtttgg gcaagaaagc cctagcactt
     1141 tcgccctctc gagtactgcc ctactccggc ttgtagccga acggcagcag cacgctcttc
     1201 gcctgcgtgt actgctcgat gccctccggc ccgttctccc gaccgatacc ggagttcttg
     1261 tagccgccga acggtgcgcc cggatcgaac gcgtacatgt tgaccgcata cgtgccggtg
     1321 cggatcttcg ccgcgatctt catcgccttc tcgttgtcgc tggtgaacac gctgcccgcg
     1381 aggccgtatg gcgagtcgtt ggcgatgcgc accgcgtcgt cctcggtgtc gtagggaatc
     1441 accgccagta ccggcccgaa gatctcctcc tgggcgatgg tcatcgagtt gtcgacatcg
     1501 gcgaaaaccg ttggctggac gaaccatccg ctgtccaggc cctcgggtcg gccgccgcct
     1561 gcgaccagcc gcgcaccttc ctcgacgccc ttgcggatgt agccctcgac gcgctcgcgc
     1621 tgcttttcgg aaatcagcgg gccgatcatc gcgccggggt cgtcgggtag gccgacaggc
     1681 attccggccg ctgcggcgct gatcttttcg acgac
//
LOCUS       DASYLX010000241         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3520490,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000241 DASYLX010000000
VERSION     DASYLX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520490"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VGP27_05910"
     CDS             complement(<1..430)
                     /locus_tag="VGP27_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HEV7850884.1"
                     /translation="MIPVTLVVIAKAPVTGQAKTRLAKTVGDQAAADIAAATLLDTLD
                     AVEATPVQARVVALTGDMGRASSSAEIGSRLDALQLVEQRGDDFSARLANAIVDAAST
                     AGPRPVLLIASDTPQVTASLLTECAQALLETDVVFGLAHDG"
     gene            complement(427..1083)
                     /locus_tag="VGP27_05915"
     CDS             complement(427..1083)
                     /locus_tag="VGP27_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7850885.1"
                     /translation="MPDATVTVVLPCLNEAASLPGVLAVLPNGYRALVVDNNSTDGTA
                     EVARLHGADVVTETRPGYGSAVHAGVQAATTPIVAVVDADGSLDPADLPALVHELDHG
                     ADMAIGRRRPVPGLRWPWHARLGTAAVCWRLRTRYGLPVHDIAPMRVAHRDPLLALGI
                     TDRRAGYPVELMVRAAKAGWTVVERDVPYGPRTGGKSKVSGSLRGSVHAGLDFWRAIS
                     "
     gene            1186..1887
                     /locus_tag="VGP27_05920"
     CDS             1186..1887
                     /locus_tag="VGP27_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7850886.1"
                     /translation="MTRVLIADDDTVVRDVVRRYLERDGLEVAIARDGNEALRLLGTQ
                     RIDVAVLDVMMPGPDGLALCRSLRQRGDHTVPVILLTALGEEDDRIAGLEAGADDYLT
                     KPFSPRELALRVRSVLRRTPAPVGVSAVELTVGELTVSTASRSVTVHAEPVSLTNREF
                     ELLLFFMTHTDTVFSREELLKQVWRWDFGDLSTVTVHVKRLRSKLGDQHRVQTVWGRG
                     YLWSSDERPHEEQTM"
     gene            1888..>2079
                     /locus_tag="VGP27_05925"
     CDS             1888..>2079
                     /locus_tag="VGP27_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7850887.1"
                     /translation="MPTIDFWEIVGWALACSVPVVLAGALIIRLARSWSLAVSMVALV
                     LIPTIATFTGVLGASGFMVT"
BASE COUNT          337 a          696 c          698 g          348 t
ORIGIN      
        1 cgccgtcgtg ggccaagccg aacacgacgt ccgtttccag cagggcctgt gcacactccg
       61 tgagcagact cgcggtcact tgcggcgtgt cggaggcgat caacagcacg ggacgaggac
      121 cggccgtcga cgcagcatcg acgatcgcgt tggccaaccg cgccgagaaa tcgtcgccgc
      181 gttgctccac cagttgcaaa gcgtccaggc gcgaaccgat ttcggcgctg ctgctggccc
      241 ggcccatgtc gcccgtcagc gccaccaccc tggcttgcac cggcgtcgct tcgacggcgt
      301 ccagggtgtc caacaacgtc gcggccgcaa tgtcagccgc cgcctggtcg ccgacggtct
      361 tggccagccg ggtcttggcc tgaccggtca ccggcgcctt ggcaatcacc accaatgtca
      421 cggggatcat gaaatcgctc gccagaagtc cagtcccgca tgaacgcttc cgcgaagcga
      481 accgcttact ttcgacttcc cgccggtgcg tggcccatag ggcacgtcgc gttcgacgac
      541 cgtccagccc gccttggccg cacgcaccat caattcgacc gggtatccgg cgcggcggtc
      601 ggtgatgccg agagcgagca acgggtcgcg gtgggcgacg cgcatcggtg cgatgtcatg
      661 caccggcagc ccgtagcggg tgcgcagtcg ccagcacact gccgcggtgc ccaggcgcgc
      721 atgccacggc caccgcaggc cgggcaccgg gcgtcgtcga cctatcgcca tgtcggcgcc
      781 gtggtcgagt tcgtggacca aggcgggaag atccgcggga tccagcgagc cgtcggcatc
      841 gacgacagca acgatcggcg tcgtcgccgc ctgtaccccg gcgtgcaccg ccgacccgta
      901 gccgggacgg gtttcggtga ccacgtcagc cccgtgcagg cgcgccacct cggcggtgcc
      961 gtccgtgctg ttgttgtcga ccaccagtgc tctataccca ttcggcagta cggccagcac
     1021 ccctggcaaa gaggccgcct cgttgagaca gggcaggacc acagtgacgg tggcgtcggg
     1081 catacctatg gacgctaggc cagcctcata accgctgcca tggtgaaccg tgacaaaagg
     1141 atgacatagc tcctggtagt gggtgcacac agctaacctc tgagggtgac gcgcgtgctg
     1201 attgccgacg acgacaccgt cgtgcgtgat gtcgtgcgcc gctacctgga acgcgacgga
     1261 ctcgaagtcg ccatcgcgcg cgacggcaac gaggcgctgc ggctgctcgg cacccagcgc
     1321 atagatgtgg cagtgctgga cgtgatgatg cccggcccgg acggcctggc gctgtgtcgc
     1381 agcctgcgcc aacgcggcga ccacaccgtc ccggtcatcc tgctgacagc gctcggcgag
     1441 gaggacgacc ggatcgccgg gctggaagcc ggtgccgacg actatctgac caagccgttc
     1501 agcccgcgcg aactcgcgtt gcgggtgcgc tccgtgctgc ggcggactcc cgcaccggtt
     1561 ggcgtgtctg cggtcgagct gacggtcggc gaattgaccg tctccacggc gtcacggtcg
     1621 gtgacggtcc acgccgaacc ggtcagcctg accaaccgcg aattcgaact gttgctgttc
     1681 ttcatgaccc acaccgacac cgtgttctcc cgcgaggagc ttctcaagca ggtgtggcgg
     1741 tgggacttcg gcgatctctc cacggtgacc gtgcatgtca agcggttgcg gtccaagctc
     1801 ggcgaccaac accgcgtgca gacggtctgg ggtcgcgggt atctgtggag cagcgatgag
     1861 cgtccgcacg aagagcagac aatgtagatg ccgacgattg atttctggga gatcgtcggg
     1921 tgggcactcg cctgctcggt cccggtggtg ttggcaggcg cgctgatcat ccggttggcc
     1981 cggtcctggt cgctggcggt cagcatggtt gcgctggttc tgattccgac gatcgcgacg
     2041 ttcaccggcg tgctgggcgc cagcgggttc atggtgacc
//
LOCUS       DASYLX010000242         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2752338,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000242 DASYLX010000000
VERSION     DASYLX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752338"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..723)
                     /locus_tag="VGP27_05930"
     CDS             complement(10..723)
                     /locus_tag="VGP27_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7850888.1"
                     /translation="MQTLRVGAAYPDPPFNGTPDGGGLDVDLMTAVADKLGMSIEFIP
                     YEGADFNGIFDALNAKEFDCVVAGTTVTPDREKKAEFVPPYLISGQSLAVNTRRQPTV
                     RSVDDLEGLTIGVQHGNTSLPIAEQLVADGKAAGIEVYDYGNIRAALADLTAGGCDAF
                     MKLAPVLTELVKPIADVEVVQRGITTEDIAVAVGLGDQELLARISVAQAELEEDGTLQ
                     RIRRKWLGNPYTDQSLGML"
     gene            complement(728..1549)
                     /locus_tag="VGP27_05935"
     CDS             complement(728..1549)
                     /locus_tag="VGP27_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850889.1"
                     /translation="MFGRADDALRYNGIAESVMHDDSVAAPFGLEALWLGAVYSALDE
                     PDRYIAHYRRQMELGRDPFELSRACVVIALLSAGRDAEAQSLADGLVEAAERTDNPYA
                     ISFALLAFAMASSMKNPLGVIDSLRRGLVTAQESGNKANVSYLAMTLAMTLGRVEGDQ
                     SGPLDNIRLAIGNYFDAGSIAQIRAALGLFMTLLDRRGDYEPAAVIGGFASVSPTAAP
                     SVAEFNEGIQHLREVLGEETYGRLAHKGEAMTMAAMVSYAYEQIDRVRGELEQLP"
     gene            complement(1495..2226)
                     /locus_tag="VGP27_05940"
     CDS             complement(1495..2226)
                     /locus_tag="VGP27_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850890.1"
                     /translation="MTAGEVRDRLDQRFRLLVGSRRGLERHQTLRHAVQWSYDLLADE
                     ERVLLERCSVFAGGFDLQSACAIAGLADDFAAIDLLDALVRKSLLVADRSSGRTRFSM
                     LETIRQFAEEQLAARGEANDVRAAHALYFAGREADMLAIWDSPRQREAYDWFATELAN
                     LRTAFRWAADQKDLDSAAAIVTLAGWFGILVENFERLPGPKRSSNRYKPRTIRGSSHR
                     AQSRRCATCSAAPMMPSAITASPNR"
     gene            complement(2204..>3262)
                     /locus_tag="VGP27_05945"
     CDS             complement(2204..>3262)
                     /locus_tag="VGP27_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850891.1"
                     /translation="HDAVLRRAIQSHGGFLFKHTGDGVIAAFASPTSAVDAAIAAQRE
                     LDLPVRMGIATGETALADGDYFGAVLNRAARVMAAGHGGQILVADSTASLVTGVDVLD
                     LGLRRLRDVPAPVGLFQVRAPGLREAFPPVRALDISPGNLRQATTSFIGRDAEVAEIK
                     DAVRTHRLITLTGVGGVGKTRLALEAATQLGDEFPDGIWLFELAAVTDPAAVPGAVAS
                     VLGITQQPGKTLAESVAAGLEGRTRLLVFDNCGHVLDAAADLVDAVLGQSPTLKVLTT
                     SREGLGAADEQGVAGPIAGHFNGGRPLRRACSQCHAKLYGRRGGDDRRHLPSPRRHPV
                     GRRVGGVADGVDDRRRGT"
BASE COUNT          583 a         1078 c         1019 g          582 t
ORIGIN      
        1 gcaggctggt cagagcatgc cgaggctctg gtcggtgtag ggattaccca gccatttgcg
       61 acggattcgc tgcagcgtgc cgtcctcttc cagctcggcc tgggcgacac tgattctggc
      121 cagaagttcc tggtcgccga gccccaccgc gactgcgatg tcctcggtgg taatgccccg
      181 ctgcacgacc tcgacatcgg cgatcggctt gacgagttcg gtcagcacag gggccagctt
      241 catgaatgca tcgcatccgc ctgcggtgag gtcggcgagg gctgcgcgga tgttcccgta
      301 gtcgtagacc tcgatgccgg ctgccttgcc gtcggccacc agctgctcgg cgatcggaag
      361 gctggtgttg ccgtgctgca cgccgatggt cagaccctcg agatcgtcga ccgagcgcac
      421 ggtcggctgc ctgcgggtgt tcacggcgag cgactgaccg ctgatcaaat agggagggac
      481 gaattccgcc ttcttctccc gatcgggcgt cacggtcgtt cccgcgacga cacagtcgaa
      541 ctccttggcg ttcagcgcgt cgaagatgcc gttgaagtcg gcgccttcgt aggggatgaa
      601 ttcgatgctc atgccgagct tgtcggcgac cgcggtcatc aggtcgacgt caagtccacc
      661 gccgtccggc gtgccgttga agggcggatc ggggtaggcc gcgccgactc tcagtgtctg
      721 catgcgctca cggtagttgt tcgagctcgc ctcgcactcg gtcgatctgc tcgtaggcgt
      781 aggacaccat cgcggccatc gtcatcgcct cacctttgtg ggcaagccgc ccgtacgttt
      841 cttcgcccag tacctcccgc agatgctgga tcccttcatt gaattcggca accgagggcg
      901 cggccgttgg actgacggat gcgaatccac cgatgaccgc cgcaggctcg tagtcgccgc
      961 gacggtcgag caacgtcatg aaaaggccaa gcgcggcacg gatctgggcg atgctgcccg
     1021 cgtcgaagta gttaccgata gcgagcctga tgttgtccaa cggcccggat tggtcgccct
     1081 cgacacggcc caatgtcatc gccagggtca tcgccagata cgagacgttg gccttgttgc
     1141 cgctctcctg ggcggtcacc agccctcggc gcaggctgtc aatcacccca agcggattct
     1201 tcatgctgct cgccatggcg aaggcgagca gagcaaagga gatcgcatac ggattgtccg
     1261 tccgctcggc ggcttcgacg aggccgtccg cgagcgattg cgcttccgca tcccgacccg
     1321 ccgagagcag ggcgatgacc acgcatgccc tgctgagctc gaacgggtca cgacccagct
     1381 ccatttgccg tcggtagtga gcgatatagc ggtccggctc gtcgagggcg gagtagaccg
     1441 caccgagcca cagcgcctcg aggccgaagg gcgctgcgac cgaatcgtcg tgcatcaccg
     1501 attcggcgat gccgttatag cggagggcat catcggcgcg gccgaacatg tagcacaccg
     1561 acgcgactgc gcacgatgag acgagcctcg gatggttctt ggcttgtaac ggttcgatga
     1621 tctcttcggc ccaggtaacc gctcgaaatt ctcgaccaag ataccgaacc aacccgccaa
     1681 cgtcacgatg gcagctgcgc tgtcgaggtc cttctggtcg gcggcccatc gaaacgcagt
     1741 gcgcagattg gccagctcgg tggcaaacca gtcgtacgcc tcccgctgtc ggggactgtc
     1801 ccaaatcgca agcatgtcgg cttcccggcc agcgaaatag agcgcgtgcg ccgcgcgcac
     1861 atcgttcgcc tcgcccctgg cggcgagttg ctcctcggcg aactggcgga tcgtctccag
     1921 catcgagaaa cgtgttcggc ccgacgagcg gtcagcgacg agcagtgatt tccgcaccaa
     1981 ggcgtccaac aagtcgatcg cggcgaagtc gtcagctaaa cctgcgatgg cacatgcgct
     2041 ttggaggtcg aatcccccgg cgaacactga acatctctcc agcagcaccc tttcctcatc
     2101 agcgagaagg tcgtaggacc actgaacggc gtgacgcagt gtctggtggc gttccaggcc
     2161 gcggcgggat ccgacgagca gccggaaacg ctgatcgaga cggtcacgta cctcgcctgc
     2221 ggtcatcgac gccatccgcg acgccgccaa ctcgacggcc aacgggatgc cgtcgaggcg
     2281 acggcagatg tcttcgatcg tcgccgcctc gtcttccgta aagctttgcg tgacactgcg
     2341 agcacgctcg acgaagaggt ctgccgccgt tgaaatgtcc agcgatcgga cccgccacac
     2401 cctgctcgtc ggcagctcca agcccttcgc ggctggttgt caggactttg agcgtcggcg
     2461 attgcccgag gacggcgtcg accagatcgg ccgcggcatc gaggacgtgt ccacaattgt
     2521 cgaacaccag caaccgagtt ctgccttcga ggcccgcggc caccgactcc gcgagggtct
     2581 taccaggctg ctgcgtgatg ccgagcaccg atgccaccgc gccgggtacg gctgccggat
     2641 cggtcactgc cgcaagctcg aaaagccata tgccgtctgg aaattcgtca cccaactggg
     2701 tagcggcttc cagggccagt cgcgtcttgc cgacgccgcc gacaccggtc agggtaatca
     2761 gccgatgagt gcggacggcg tctttgatct cggcgacttc cgcatcgcgt ccgatgaagc
     2821 tcgtcgtcgc ctgccgtagg ttgcccgggc tgatgtcgag tgcacgcacc ggcgggaacg
     2881 cctcgcgtag acctggtgcg cggacttgaa acaagccgac cggtgccggt acatcgcgaa
     2941 gtcgccgcag tccgaggtcc agcacgtcca cgccagtgac cagggaagcc gtcgagtcgg
     3001 ccaccaagat ctgtccacca tgccctgccg ccatcacccg ggccgcgcgg ttgagcaccg
     3061 cgccgaagta atcgccgtcc gcaagcgccg tctcaccggt ggctatcccc atccgcacag
     3121 gcaaatccaa ctctcgctgc gccgcaatcg ccgcatccac tgcggacgtg ggcgatgcga
     3181 acgcagcgat cacgccgtca ccggtgtgct tgaacagaaa tccgccatgc gactggatcg
     3241 ctctgcgcag aacggcatcg tg
//
LOCUS       DASYLX010000243         8765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_558902,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000243 DASYLX010000000
VERSION     DASYLX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558902"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VGP27_05950"
     CDS             <1..1086
                     /locus_tag="VGP27_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850892.1"
                     /translation="LVGSTFDELLADEPLVGTVAADPASPALIAFTSGTTSDPKGVVH
                     SHQTLSFETRQLHANYPPDRGRQLTATPVGHFIGMLGAFLIPVLEGAPIDLADVWDPG
                     NVLRLIESHGLSIGGGPPYFVTSLMDHPDCTPAHMAHFKTVGFGGSTVPSAVTRRLAD
                     MGIFVFRSYGSSEHPSITGSSRDAPEDKRLYTDGNARPGVEIRLGPDGEIYSRGPDLC
                     LGYTDDALTAQAFDADGWYRTGDVGVLDDDGYLTITDRKADVIIRGGENISALEVEEL
                     LLTMPAVAEAVVVSAPDARLGEHAAAVLRVKPGHAMPTMAEVHDHFGRAGVAKQKWPE
                     ELREVDDYPRTASGKVQKFVVRQNLSA"
     gene            1179..2369
                     /locus_tag="VGP27_05955"
     CDS             1179..2369
                     /locus_tag="VGP27_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850893.1"
                     /translation="MGQLSHRVDIPFPLFDADNHLYEPPEAMTKYLPKEYKDVVQYVE
                     VNGRTKIALRGVISNYIPNPTFSVVAKPGAWEEYFKFGNPDGKSKRELFGEPMRAIPA
                     FFEPGPRLEKMNELGIERSLMFPTLASLIEERLSDDPVAIHVLIHSLNQWLDEVWGFN
                     YQNRIFTTPVITLPIVEKAIEELEWCVKRGARAILVRPAPVPGFRGPRSFALPEFDPF
                     WQKCVEYDVFVGMHSSDSGYSRYTSEWDGASQEMLPFQTNAMSILNEWRPIQDAVASW
                     VIHGALFRFPELKVGIVEAGSKWMFPLLDSMAEVYKKAPEAFLGNPIEEIKNRIYVSP
                     FYEEGIDDLINLIGVEQVLYGSDWPHPEGLAEPTHYVTALEHLAVEDQAKIMGGNLGR
                     LVTV"
     gene            2366..3802
                     /locus_tag="VGP27_05960"
     CDS             2366..3802
                     /locus_tag="VGP27_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HEV7850894.1"
                     /translation="MTQPSWQTIPEMVLSAADRFGDAEAVVDGSLRLSFAEVVRRIRC
                     AAGAFNDLGIDNGERVAVWAPNSADWMIAAFGLLTAGGVLVPVNTRFKAEEAADIIGR
                     SGAKAVLIQQGFLGYDYTAPAGVPVIDLKSDFLSSGSPFAREVNGTDISDIIYTSGTT
                     GRPKGVMMNHSQNLRLYTEWCELADLREGDRYLMVNPYFHTFGFKAGLIASFTRGATM
                     VPVPVFDVDRVVELIEAERITMLPGPPTLYHSLLGVEDKSKLKTLRAGVTGSADIPVE
                     LIRRVHEELPFQTLATGYGLTEAGTVTLSRPGDSFSDIATTAGVPCDGVEVRIADDSE
                     VLVRGYSVMQGYLDDPVATAEAIDGDGWLHTGDLGRFNDDGRLMIVGRKKDMFIVGGF
                     NAYPAEIEGFLLEHPAVAQAAVIGVPDDRLGQVGKAFVVLKGAATAEELISWSRERMA
                     GFKVPRYVEFLDKLPLGATGKVMKDRLR"
     gene            3945..4877
                     /locus_tag="VGP27_05965"
     CDS             3945..4877
                     /locus_tag="VGP27_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850895.1"
                     /translation="MPSFRRRASVIDVDRVDGPPQGPGKPADAAEALALAEEAEAEAA
                     EAEALATAARARARAIRLRRQAQDAEGEASADVEAAAEVETNADAPEVASPDAPQGDT
                     ETSDVVAAPAQASPVDAAKSDTEAIKKDIAEEPAEAEPAGKRRRWRAGRILKTIAAAI
                     AVACTAALITLSVLMILDHRHQVTDQQRRAQYAAAARQSVVTLMSLDFNKAKEDVQRI
                     IDNSTGAFRDDFKNQADDFIKVAQESKVVTEVNVNATAVESMSDDSAVVLVSASSRVT
                     NVAGAKQEPRSWRLSVDLKRDGDQLKMSKVEFVP"
     gene            4874..5524
                     /locus_tag="VGP27_05970"
     CDS             4874..5524
                     /locus_tag="VGP27_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850896.1"
                     /translation="MTADEAARVTDVDEAENGTEESVDDVIAHDDVAAEQETDTKPES
                     RDGIVRRSVRVGWARLGVVLLAVALVASAGLAAWLYFKQYRPDEQTNAAASAVALDAA
                     KNGTVSLLSYSPDKLDTDFATAKSHLTGDFLSYYTQFTEQVVTPAAKQKSVKTSATVV
                     RAAVSDMHPDSAVILVFINQVTTSKENPDGAFAASAVKVGLKKISDAWLISSFDPV"
     gene            5539..6489
                     /locus_tag="VGP27_05975"
     CDS             5539..6489
                     /locus_tag="VGP27_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7850897.1"
                     /translation="MLVVDLGDPPTDSPARTGVLVAVGSADDIASPPAQDWLERATLT
                     LSEDAVADRRVVTVESTAAALAELSTRFETWPQAATICADVLRSAHAQGPALAGVITE
                     SLAYSTLQSGGEFAAWLEHRGAAAAPVLPDPVVADRDGDTLHIRFNRPSRHNAFSNDA
                     RAALLEALDVARHDPSIAEVILSGNGPSFCSGGDLAEFGSFADPASAHIVRVRHSPAL
                     VLDELTARLGERCRARVHGQVLGSGLEMAAFCGWVGAHSDSVLGLPELSLGLIPGAGG
                     TVSVTRRIGRWRTAYLVLSGRTIDADTALRWGLVNAIEAS"
     gene            6509..7633
                     /locus_tag="VGP27_05980"
     CDS             6509..7633
                     /locus_tag="VGP27_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7850898.1"
                     /translation="MGSLGIPDAVLASARQTADGIERSSAHLGGAVDVDADEILTGRA
                     GLLGLAPAGRVSAGRATRLLSTRDGWCALTLSRIDDVEAVPALLELDDQPTDPWAALR
                     AAAVGRGADEFVARARMLDLPAAVLGETPPAAPRSVPNGHRAQRRLSDLLVADLSSMW
                     AGPLCGHLLAGAGATVVKVESRDRPDGTRGGDPRFFDWMNASKLSYAVDLRDDGLRPL
                     LEAADVVIEGSRPSALTRHGLGPGQVPGRAGRVWLRVTGYGTEGECAERVAFGDDAAV
                     AGGLVGVGVVFVGDAIADPLTGLAATQAVLDSLARGGGETVEAALAEVAAVYATSPSM
                     VSPRNGEPPRVPAPPSKPAARLGADNQRVRELIENRLGPC"
     gene            7627..>8765
                     /locus_tag="VGP27_05985"
     CDS             7627..>8765
                     /locus_tag="VGP27_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7850899.1"
                     /translation="MLIRRGRLPDGRVADLRVDERIVEIADRLDPVAAEQVVDADAGA
                     VLPGLHDHHLHLRAMAAALDSLSVGPPQVRTKARLAQALRTAEPGPDGWIRAVGYHAS
                     VAGELDRDQLDALVADTPVRVQHRSGAMWILNSAALSRVGLADHPDGRLRSSDDWAHA
                     LPRRVTALADITHRLAGYGVTGITDATPDLTAEDVATLSVAHRRGEIPQRLHFLAPGK
                     RILHDDRLDVDELIGWVSDHHDDDIPVALHCVTHAQLVVALAALRSAGGHRLDRIEHA
                     AVVPDEMLADLVDVGVTVVTQPNFVAERGEHYLRDVPVGEHPQLWRVASLMRAEIPLA
                     ASTDAPFGGMDPWAAMRAAVHRTTAAGDVLGPIERITADEALSLFL"
BASE COUNT         1487 a         2871 c         2965 g         1442 t
ORIGIN      
        1 ttggtgggaa gcaccttcga cgaactgctg gccgatgagc cgctggtggg cactgtcgcg
       61 gcggatccgg caagcccggc gttgatcgcc ttcacctcgg gcacgacgag cgatcccaag
      121 ggtgtcgtcc acagccacca gacgctcagc ttcgaaaccc gccagctgca tgcgaactat
      181 ccgcccgacc gcggcagaca gctcaccgca acgcctgtcg gacacttcat cggcatgctg
      241 ggcgcatttc tgatcccggt gctcgagggt gcgccgatcg accttgccga cgtgtgggat
      301 ccgggcaacg tcctgcggct catcgaaagc cacggcctgt cgatcggcgg cggcccgccg
      361 tacttcgtca ccagtctgat ggaccatccc gactgcacac ccgcacacat ggcacacttc
      421 aaaaccgttg gcttcggcgg atctacggtg ccctcggcgg tcacccgcag gcttgccgat
      481 atgggcatct tcgtcttccg gtcctacggc agttccgagc atccgtcgat cacgggatcg
      541 agtcgcgatg ccccagagga caaacggctc tacaccgacg gcaatgcacg gccgggcgta
      601 gagatccggc tcggacccga cggtgaaatc tacagccgcg gacccgatct gtgcctcggc
      661 tacaccgacg acgcgttgac cgcacaagcc ttcgacgccg acggctggta tcgcacaggc
      721 gatgtcggcg tgcttgacga cgacggctat ctgaccatca ccgaccgcaa ggccgatgtc
      781 atcattcgcg gcggcgagaa catcagcgcg ctcgaggtcg aagaactgct gctgacaatg
      841 cccgccgtcg ccgaggccgt cgtggtgtcc gcgcctgacg ccaggctcgg cgagcacgcg
      901 gccgccgtgc tgcgggtcaa gccgggccac gccatgccga cgatggccga agtgcacgat
      961 cacttcggtc gggcgggggt ggccaaacag aaatggccgg aggagttgcg tgaagtcgac
     1021 gactatccgc gcacggcgag cggcaaggtc cagaagttcg tggttcgcca gaacctgtcg
     1081 gcctgagttc gccagaacct gtcggcctga gtcagggagc gcattgcgtt gcgcagttga
     1141 tgagaatagg attctcctaa gaggataagg agtatttcat gggccaactt tcgcatcggg
     1201 tcgacatccc gtttccgctg ttcgacgctg acaatcacct ctacgagcca ccggaggcga
     1261 tgaccaagta cctgccgaag gagtacaagg acgtcgtgca gtacgtcgag gtcaacggcc
     1321 gcacgaagat cgcgctgcgc ggtgtgatca gcaattacat cccgaacccg accttctcgg
     1381 tggtcgccaa gccgggcgcc tgggaggagt acttcaagtt cggcaaccct gacgggaaga
     1441 gcaagcgcga actgttcggc gagccgatga gggccatccc ggctttcttc gagcccggtc
     1501 cgcgactcga gaagatgaac gaattgggca tcgagcgctc gttgatgttc ccgacgctgg
     1561 ccagcctgat cgaggagcga ctctccgatg acccggtcgc catccacgtc ctcatccatt
     1621 cgctgaacca gtggctcgac gaggtctggg gcttcaacta ccagaaccgc atcttcacca
     1681 cgccggtgat caccctcccc atcgtcgaga aggccatcga ggagttggag tggtgcgtca
     1741 agcgcggcgc ccgcgcgatc cttgttcgcc cggcgccggt gcccggattc cgcggcccgc
     1801 gctcgttcgc gctgccggag ttcgacccgt tctggcagaa gtgcgtcgag tacgacgtgt
     1861 tcgtcggcat gcattccagt gacagcggtt attcccgcta cacctccgag tgggacggtg
     1921 cctcccagga aatgctgccg ttccagacca atgccatgtc gatcctcaac gagtggcgtc
     1981 cgatccagga cgcggtggcc tcgtgggtga ttcacggcgc gctgttccgg ttccccgaac
     2041 tgaaggtggg catcgtggaa gccggctcga agtggatgtt cccgctgctg gactccatgg
     2101 ccgaggtcta caagaaggcg ccggaggcct tcctcggtaa cccgatcgag gagatcaaga
     2161 accgcatcta cgtcagtccc ttctacgagg agggcatcga cgacctgatc aacctgatcg
     2221 gagtggagca ggtgctctac ggctccgact ggccgcaccc ggagggactg gcggagccga
     2281 cccactatgt gaccgcgctc gaacatctcg ccgtcgagga ccaggcgaag atcatgggcg
     2341 gcaacctggg tcgtctcgtc acggtgtgac acaaccctcc tggcagacca tccccgagat
     2401 ggtcctgagc gcggcggacc gtttcggcga cgcggaggcg gtcgtcgacg gttcgttgcg
     2461 cttgtccttc gccgaggtgg tccgccggat ccgttgtgcg gcaggcgcct tcaacgacct
     2521 ggggatcgat aacggtgagc gggtcgcggt ctgggcgccg aactccgcgg actggatgat
     2581 cgccgcgttc ggtctgctga ccgcgggcgg tgtgctcgtt cccgtcaaca cccgcttcaa
     2641 ggcagaggaa gccgctgaca tcatcggcag aagcggtgcc aaggcggtgc tgatccagca
     2701 gggcttcctc ggctatgact acaccgcgcc tgcgggcgtg ccggtgatcg acctgaagtc
     2761 cgacttcctc tccagtggtt cgccgttcgc ccgcgaggtg aacggcaccg acatctccga
     2821 catcatctac acctcgggca ccacgggcag gcccaagggc gtgatgatga accactcgca
     2881 gaaccttcgg ctgtacacgg aatggtgtga gctggccgat ctgcgcgagg gcgaccggta
     2941 tctgatggtc aacccgtact tccacacctt cgggttcaag gcggggttga tcgcgtcgtt
     3001 cacccgcggc gcgacgatgg tgccggtgcc ggtattcgac gtcgaccggg tggtggaact
     3061 gatcgaggcc gaacggatca caatgctgcc cgggcctccg acgctttatc actcgctgct
     3121 gggggtcgag gacaagagca agctgaaaac gctgcgcgcc ggggtgaccg gctcggcgga
     3181 catccccgtc gagctgatcc ggcgcgtgca cgaggagctg cctttccaga cgctggcgac
     3241 cggctacggc ctgaccgagg ctggcaccgt gacactgtcg cggccggggg actcgttctc
     3301 cgatatcgcc accaccgcgg gcgtgccgtg cgacggggtg gaggtgcgca tcgccgacga
     3361 cagtgaggtg ctggtccgcg gctacagcgt catgcagggc tacctcgacg accccgtcgc
     3421 gacggcggaa gcgatcgacg gcgacggctg gttgcacacc ggcgacctgg gcaggttcaa
     3481 cgacgacggc cggctgatga tcgtgggccg caagaaggac atgttcatcg tcggcggctt
     3541 caacgcctac ccagcagaga tcgagggctt cctgctcgag catcccgccg tcgctcaggc
     3601 cgccgtgatc ggtgtgcccg acgaccgtct cggacaggtc ggtaaggcgt ttgtcgtgct
     3661 caagggtgcc gcgaccgcgg aggaactgat ctcatggagc cgggaacgga tggctggctt
     3721 caaggtcccg cggtacgtag agtttctgga caagctgccg ctgggcgcga caggcaaagt
     3781 gatgaaggac agattgcgct gagcgttcgc acagctggga aatagcattt ccgcaggaaa
     3841 ggcggcccgg ctccgatctg ggcatgctgt atcgtcgcgg cgatactcaa aaatgaataa
     3901 tgccattctc attacgtagg gcactcacag acaaggaggt cgctgtgccg tctttcaggc
     3961 gccgcgcgtc ggtcatcgac gttgatcgcg tcgatggacc gccgcagggt cctgggaagc
     4021 cggctgatgc ggccgaagca ctggcactcg cggaggaagc agaagcggag gcggccgagg
     4081 ccgaggcgct tgccactgcc gcccgcgcca gagctcgcgc gatccggctg cggcggcagg
     4141 cccaagacgc agaaggagaa gcgtccgccg acgtcgaagc agccgccgag gtcgaaacga
     4201 acgccgatgc gccggaggtg gcatcgccag acgcgcccca aggcgatacc gaaacgtctg
     4261 acgtcgtggc cgcaccggcc caggcgagtc cggtcgacgc tgccaaatcg gacactgaag
     4321 ccatcaagaa agacatcgca gaggagcccg cggaagcaga gcccgccggc aagcgacgtc
     4381 gctggcgagc cggtcgcatc ctgaagacca ttgcggccgc tatcgctgtc gcctgcaccg
     4441 ccgcattgat tactttgagc gtgctcatga tcctggacca ccgccaccag gtgaccgatc
     4501 agcagcgccg cgcgcagtac gccgcagcag ctcggcagag cgtcgtgaca ctgatgtcgc
     4561 tggacttcaa caaggccaag gaggacgtcc agcgcatcat cgacaactcg acaggtgcgt
     4621 tcagagacga cttcaagaac caagcggacg acttcatcaa ggtggcgcag gagtcgaagg
     4681 tggtcaccga ggtcaatgtc aacgccaccg cggtcgaatc gatgagcgac gattcggccg
     4741 tcgttctcgt gtccgccagt tcgcgcgtca ccaacgtggc cggggccaaa caggagccgc
     4801 ggtcatggcg gttgtcggtc gacctgaagc gtgacggcga ccagctgaag atgtcgaagg
     4861 tggagttcgt accgtgaccg cagacgaagc agcccgtgtg acagatgtcg acgaggccga
     4921 gaacggcaca gaagaatccg tcgacgacgt tatcgcccac gacgacgtgg ccgccgaaca
     4981 ggaaacggat acgaagccgg agtcgcgaga cggcattgtg cgtcggtcgg tacgggtggg
     5041 gtgggcccgg ctgggtgtag tgctgttggc ggtcgcgctc gtggcctctg caggcctcgc
     5101 ggcgtggctg tatttcaagc agtaccggcc tgacgaacag acgaacgcgg ccgcgtcggc
     5161 cgtcgcgctg gacgcggcga agaacggcac ggtttcgctg ctgtcgtatt caccggacaa
     5221 gttggacaca gacttcgcga cagcgaagtc ccacctgacc ggtgatttcc tgtcgtacta
     5281 cacgcagttc acggagcagg tcgtcactcc ggcggcgaag cagaagtcgg tgaagacttc
     5341 tgcgacggtc gtgcgggctg ccgtttccga catgcatccg gactccgcgg tgattctcgt
     5401 gttcatcaac caggtcacca ccagcaagga gaatcccgac ggtgcgttcg ccgcgagcgc
     5461 ggtcaaggtg ggcttgaaga agatcagcga tgcctggctg atctcatcct tcgacccggt
     5521 gtagcgccgt gcggggccgt gctcgtcgtt gacctcggcg acccgccgac cgattcgcct
     5581 gcccggaccg gtgtgcttgt ggcggtcggg tctgccgacg acatcgcatc accgcccgca
     5641 caagactggc tcgagcgcgc caccctcacc ctgagcgagg acgctgtcgc cgaccgccgg
     5701 gtcgtcaccg tcgagtcgac ggcggccgcg ctcgccgagc tgagcacccg cttcgagacg
     5761 tggccacagg cggccacgat ctgtgccgac gtgcttcgct cggctcatgc gcagggtccc
     5821 gcgctggccg gggtgatcac ggagtcgctc gcctactcga cgctgcaatc cggcggcgag
     5881 ttcgccgcgt ggttggagca ccggggtgcc gccgccgcgc cggtccttcc ggatccggtg
     5941 gtggccgacc gtgacggcga cacactgcac atccggttca accggccgtc gcgccacaat
     6001 gcgttctcca acgacgccag ggccgcgctc ctggaggccc tcgacgtcgc ccgtcacgac
     6061 ccgtcgatcg ccgaggtgat cctgtccggc aacggcccgt cgttctgcag cggaggcgac
     6121 ctcgccgaat tcggttcctt cgccgacccg gcgagcgcgc acatcgtacg ggtgcggcac
     6181 agccccgcgc tggtgctcga cgagctcacc gcccggctgg gcgagcgctg ccgagcgcgc
     6241 gtgcacggcc aggtactcgg cagcgggttg gagatggccg cattctgcgg atgggttggc
     6301 gcgcatagtg attcggtgct cggcctaccc gaactgagcc tcggcctgat cccgggcgcc
     6361 ggtggcacgg taagcgtgac gcgacggatc ggccgttggc gcacggctta tctggtgttg
     6421 tcggggcgca cgatcgatgc ggacaccgca ctgcgctggg gtctggtgaa cgcgatcgag
     6481 gcgtcgtagt cccatgcaca atgtgtcggt gggttcgttg ggcattcccg atgccgtgct
     6541 cgcttcggcg cgtcagacgg ccgacggcat cgagcgcagc tcagcccacc ttggtggcgc
     6601 cgtggacgtc gacgccgacg agatcctcac cggccgagcc ggattgctcg ggctggcgcc
     6661 cgccgggcgg gtttcggcgg gccgcgcgac gcgactgctg tccaccagag acgggtggtg
     6721 cgcactcacg ctgtcgcgca tcgacgacgt cgaggcggtt cctgcgcttc tcgagctcga
     6781 cgatcagcca accgatccgt gggcggcgtt gcgggcggcg gccgtcggcc gcggcgcgga
     6841 cgaattcgtc gcacgggcgc gaatgctcga cctacctgcc gcggtgctcg gtgaaacccc
     6901 gcccgcagca ccgcgatcag tgcccaacgg gcatcgggcc caacggagac tgtccgacct
     6961 gctggtcgcc gacctgtcgt cgatgtgggc gggaccgctg tgcggacatc tgctcgccgg
     7021 cgccggggcc accgtcgtga aggtggagag ccgcgacagg cccgacggca cacgcggggg
     7081 ggaccccaga ttcttcgact ggatgaacgc ctcgaagttg tcctacgccg tcgatcttcg
     7141 tgacgatgga ttgcgtccgc ttctcgaggc cgccgatgtc gtcatcgagg ggtcgcggcc
     7201 ctcggcgctg acccgccatg gcctggggcc cgggcaagtg ccgggtcggg ctggccgagt
     7261 ctggttgcgc gtcaccggat atggcaccga gggcgagtgc gccgagaggg tggctttcgg
     7321 tgacgacgcc gccgtcgcgg gcggtttggt gggcgtgggc gtggtcttcg tcggcgacgc
     7381 gatcgctgac ccgctgaccg gtttggcggc cacgcaggca gtgctcgatt cgctcgcgcg
     7441 cggcggcggc gaaaccgtcg aggcggccct cgcggaggtg gccgccgtct atgccacgtc
     7501 accgtcgatg gtcagtccga gaaacggcga accgccgcgg gtgcccgcgc cgccgtcgaa
     7561 accggcggct cggctgggcg cggataacca gcgcgtgcgc gaactgatcg aaaaccggct
     7621 tggcccgtgc tgattcgccg tggcagattg cccgatggtc gcgtcgccga cctgcgggtc
     7681 gacgagcgca ttgtcgaaat cgccgaccgg ctcgaccccg ttgccgccga acaggtggtt
     7741 gatgccgacg ccggcgcggt actgcccgga ctgcacgacc atcatctgca cctgcgagcg
     7801 atggccgccg cgctcgactc gctgagcgtc gggccgccgc aggtccggac gaaagcgcga
     7861 ctggcgcagg cgttgagaac ggccgaaccg ggaccggacg gctggatccg ggcggtcggc
     7921 taccacgcgt cggtcgcagg tgaactcgac cgcgaccagc tcgacgcgct cgtcgccgac
     7981 acaccggtac gggtccagca ccgcagcggt gccatgtgga tcttgaactc ggccgcgctg
     8041 tctcgcgtcg gcctcgccga tcatccggac ggccggctgc gcagctccga tgactgggcg
     8101 cacgcgttgc cgcgccgggt gaccgcactc gccgacatca cccaccgcct cgccggatac
     8161 ggcgtcaccg gaatcaccga tgccacacca gatctcaccg cggaggacgt ggcgacgtta
     8221 tcggtggcac atcggcgcgg cgaaattccg cagcggctgc acttcctcgc gccgggcaag
     8281 cggatcctgc acgacgaccg gctcgacgtc gacgaactca tcggttgggt cagcgatcat
     8341 cacgacgacg acatcccggt cgcgctgcac tgcgtgaccc acgcccaact ggtcgtcgcg
     8401 ctcgccgcgt tgcggtcggc gggcgggcat cgcctcgacc gcatcgagca cgccgccgtc
     8461 gtgcccgacg agatgctcgc cgaccttgtc gacgtcgggg tcaccgttgt cacccagccc
     8521 aacttcgtcg ccgaacgcgg cgagcactac cttcgcgacg tgcccgtcgg cgaacacccg
     8581 cagttgtggc gggtggcgtc gctgatgcgg gccgaaatcc cgctcgccgc gagcactgac
     8641 gcgccgttcg gcggcatgga tccgtgggcg gcgatgcgcg cggccgttca tcgcaccacc
     8701 gcggccggag acgtcctcgg tcccatcgaa cgcattacgg ctgacgaggc actgagcttg
     8761 ttcct
//
LOCUS       DASYLX010000244         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2204119,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000244 DASYLX010000000
VERSION     DASYLX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204119"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..538)
                     /locus_tag="VGP27_05990"
     CDS             complement(2..538)
                     /locus_tag="VGP27_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV7850900.1"
                     /translation="MVRLLLIVVLVLAVLVLVGFVMGYNKIRSADVRVAEALSGIDVE
                     LTRRAALIPSLVHIVQTFAAHEKDILDHVTDARAALTTATTGKSVAQRSSAEQDFDSA
                     VAPVLALGQSYPQLTSSNNFSSLQDSLADTESKLAFARGYYNDSAATLNRLLRTMPWM
                     FVAPLSGVSEREYYKIPR"
     gene            complement(532..2373)
                     /locus_tag="VGP27_05995"
     CDS             complement(532..2373)
                     /locus_tag="VGP27_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV7850901.1"
                     /translation="MRRIIVWSISLLLIMFGLLWPLVLKGGQDELPADDPVVFSNFNA
                     DFHVNADGSLDAVETITAEFPSGRHGLFQWWDTSNQNHPYVRQTPEITSVKVDGAPAP
                     YRMLWMDGKRFRVAKIGDPDEFLSFGSHVFEIRYTVPGVLDPGDTGSDKRFAESAGAD
                     AAAQSVFFWNVVAGSWNNRMQHVDISVTLPSAVTGAQCSVGYGVGSPCGALTITGNKV
                     ALSTDYLGSRTPVTLRAGVDVPTPPREELLWPYTWDRIIGTSLGDMAWVLSLTVAFAL
                     GGFLWYRTTVEPPPGFPLQYSPPPGLGPVQTEYIRTEAVPKNALAATLFYLAERKLVD
                     LRQISDEHWRIKGLADKSDWERLDPVSRTVGNALRVNVAGEVFEAKKSIKSGQKLNKA
                     KTDIAKAVVQWAFGGGLMVKHRKELWVRAANVIAIPVAVCCFFLWFGIPITALGLPFA
                     AFFLVTVRSWIGGVGTRRTAAGRELWSQAGGFHRMLVTDSAEARFDFSARKELYTAYV
                     PFAVAAGAAALWAKKYEMTTGTAAPQPDWYNSSSSSSTSEGWGFTGGSGGANFDSFDS
                     ALTSSIGAYTASQSSSSSGGSSGGSSGSSGGGSGGGGGGGGGGGGSW"
     gene            complement(2411..>3434)
                     /locus_tag="VGP27_06000"
     CDS             complement(2411..>3434)
                     /locus_tag="VGP27_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244827.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HEV7850902.1"
                     /translation="SRAPRVIALEDSGYAHERDPAAHSARLPSMALQATRTALDAGHP
                     VLVQVPRRGYVPALACGRCRTIARCRHCTGPLSLPDRNASGAVCRWCGREEPSLRCAR
                     CGSDAVRAIVVGARRTAEELGRAFPGTTVVTSGGDTIVTTVAGDPALVVATPGAEPVA
                     AAGYGAALLLDPWALLGRQDLRAAEDTLRRWMAASTLVRSRSDGGVVAVVADSTVPTV
                     QALIRWDPVGHAEAELDARDEVGLLPGVHMAATDGAPEAVAALLDTAELPASAELLGP
                     VDLPPGARRPPGVPATSPVNRMLVRVPRADGLSLAAALRRATGVLSARHDQLPVRVQI
                     DPLHIG"
BASE COUNT          578 a         1199 c         1137 g          520 t
ORIGIN      
        1 gctaccgcgg gatcttgtag tactcgcgtt cggacacgcc gctcagcggc gcgacgaaca
       61 tccacggcat ggtgcgcagt agccggttca gcgtggcggc cgagtcgttg tagtaaccgc
      121 gggcgaacgc caacttgctc tcggtgtcgg cgagactgtc ctgcaggctt gagaagttgt
      181 tcgacgaggt gagctgcggg tagctctggc cgagcgccag caccggcgcc accgcggagt
      241 cgaaatcctg ctcggcggag ctgcgctggg cgacggactt gcccgtcgtc gccgtcgtca
      301 acgcggccct ggcatcggtg acgtggtcga ggatgtcctt ctcgtgggcg gcgaacgtct
      361 gcactatgtg caccaggctc gggatcagcg ccgcacggcg ggtcaactcc acatcgattc
      421 cggagagcgc ctcggcgacc cggacatcgg cgctgcgaat cttgttgtag cccattacga
      481 aaccgacaag cacaagaacc gccagcacca gcacgacgat cagcagcagc cttaccacga
      541 tccgcctcct cctccgccgc cgccaccgcc gccgccgcta ccgccaccgc tgctaccgct
      601 gctaccgcca ctgctaccgc cggacgacga cgatgactgc gatgcggtat aggcaccgat
      661 cgacgatgtc aacgccgagt cgaagctgtc gaagttcgca ccaccggaac ccccggtgaa
      721 accccagccc tccgacgtcg atgacgacga cgacgagttg taccaatccg gctgtggcgc
      781 agccgtaccc gtcgtcatct cgtacttctt cgcccacagc gccgccgcac ccgccgcgac
      841 ggcgaacggc acataggcgg tatagagctc cttgcgggcc gagaagtcga agcgtgcctc
      901 ggccgaatcg gtgaccagca tccgatggaa tccccctgcc tgcgaccaca gttcgcgacc
      961 ggccgcggtg cgacgggtac ctacgccgcc gatccacgac cggacggtga ccaagaagaa
     1021 tgcggcgaac ggcagaccca gcgcggtgat cggaataccg aaccacagga agaagcagca
     1081 caccgccaca ggaatcgcga tcacattggc cgcgcgcacc cacagctctt tcctgtgctt
     1141 gaccatcagg ccgccgccga aggcccactg caccacggcc ttggcgatat cggtcttggc
     1201 cttgttgagc ttctgaccgg acttgatgga cttcttcgcc tcgaacactt cccctgccac
     1261 gttcacccgc agcgcgttcc ctaccgtgcg gctgaccgga tcgagccgct cccagtcgct
     1321 cttgtcggcc aggcctttga ttcgccagtg ctcgtcgctg atctgtctca ggtcgaccag
     1381 cttgcgctcg gcgaggtaga acagcgttgc ggcgagggcg ttcttcggga cggcttcggt
     1441 gcggatgtat tcggtctgca ccggtccgag tccaggcggc ggcgagtatt gcagcgggaa
     1501 gccgggcggc ggttcgaccg tggtgcgata ccacaggaag ccgccgagcg cgaaggccac
     1561 cgtcaggctc agcacccacg ccatatcgcc caacgacgta ccgatgatcc ggtcccatgt
     1621 gtacggccac aggagttcct cacgcggtgg ggtcggcacg tcgacgcctg cccgcaacgt
     1681 caccggagtc cgggagccca ggtaatcggt cgaaagcgcc accttgttgc cggtgatggt
     1741 gagggcgccg cagggtgaac cgacgccgta gccgaccgaa cactgcgcgc cggtgacagc
     1801 gctgggcagt gtcaccgaga tgtcgacatg ctgcatccgg ttgttccacg aaccagcgac
     1861 gacgttccag aagaacaccg actgcgccgc cgcgtcggcc ccggcggact cggcgaaccg
     1921 tttgtcgctg ccggtatcac cgggatccag cacacccggc accgtgtagc ggatctcgaa
     1981 cacgtgtgac ccgaaactga ggaactcgtc gggatcgccg atcttggcca cccggaaccg
     2041 cttgccgtcc atccacagca tgcggtacgg cgcgggagcg ccgtcgacct tcaccgacgt
     2101 gatctcggga gtctgccgga cgtacgggtg gttctggttg gaggtgtccc accactggaa
     2161 caggccgtgg cgcccgctcg ggaactcggc cgtgatggtc tccaccgcgt cgaggctgcc
     2221 gtctgcgttg acatggaagt cggcgttgaa attgctgaac accaccgggt cgtcggcggg
     2281 caactcgtcc tggccaccct tgagtaccag cggccagagc aacccaaaca tgatgagcag
     2341 caacgaaatc gaccacacga ttatccgccg catgtcacct cccgaagagc cccggtgccg
     2401 aagtctcgca ttaccctatg tgcaacgggt cgatttgcac ccgaacgggc agctgatcgt
     2461 gccgcgcgct gagcaccccg gtggcccgcc gcagcgccgc cgccagcgaa agaccgtcgg
     2521 cccgcggcac ccgcaccagc atccggttga ccgggctcgt tgcgggaacg ccgggcggtc
     2581 tgcgggcccc gggcggcagg tccacgggcc cgagtagctc cgcggaggcc ggcagttcag
     2641 cggtgtcgag cagcgccgcc accgcctccg gcgcgccgtc ggttgccgcc atgtgcacac
     2701 cgggtagcag cccgacctcg tcgcgggcgt ccagttcggc ttcggcatgt ccgaccggat
     2761 cccagcggat cagtgcctgc acagtcggca ccgtcgagtc ggcgacgacc gcgaccacgc
     2821 cgccgtcgct gcggctgcgg accagggtgg atgcggccat ccagcggcgc agcgtgtcct
     2881 cggccgcacg cagatcctgg cgccccagca gcgcccatgg gtcaaggagc agcgcggcgc
     2941 cgtagccggc ggccgccacg ggttcggcgc ccggggtggc gacgacgagc gctggatcgc
     3001 cggccaccgt tgtgacgatg gtgtcgcccc cggaggtgac gacggtggtg ccggggaacg
     3061 cccggcccag ttcctcggcg gtgcgccggg cgccgacgac gatggcgcgg acggcatcgg
     3121 acccgcatcg ggcacagcgc agcgagggtt cctcgcggcc gcaccaccgg cacaccgcgc
     3181 ctgaggcatt ccgatcgggc agcgacagcg ggcctgtgca gtgtctacag cgggcgatgg
     3241 tgcggcagcg gccgcaggcc agcgccggga cgtaacccct gcgtggcacc tggacgagaa
     3301 cgggatggcc ggcgtcgagc gccgtgcgtg tcgcctgcag cgccatcgag ggcagccgcg
     3361 cgctgtgtgc ggcggggtca cgctcatggg cgtatccgct gtcctcgagg gcgatcaccc
     3421 gcggtgcccg cgac
//
LOCUS       DASYLX010000245         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_229625,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000245 DASYLX010000000
VERSION     DASYLX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229625"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VGP27_06005"
     CDS             complement(<1..163)
                     /locus_tag="VGP27_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850903.1"
                     /translation="MGQQCVFSDMVPMPLPELFRRWYGPIPPIKETRNQTGDWDAVGQ
                     SRTVVLVGGG"
     gene            complement(215..1264)
                     /locus_tag="VGP27_06010"
     CDS             complement(215..1264)
                     /locus_tag="VGP27_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7850904.1"
                     /translation="MGLRVVQWATGGVGVAAIKGVLEHPELELVGCWVHSADKAGRDV
                     GDLIGTEPLGVTATNSIDDIVALDADAVIYAPLMADPDEVAALLRSGKNVVTPVGWLY
                     PSERSAAPMREAALEGDATLHGTGMAPGGISEKFPLLFSAFSTGVTFVRAEEFSDLRS
                     YDAPDVLRHVMGFGEVPEKALTGPMQKMLDSGFIQAVKMVVAKMGFNADPKVRATQEV
                     ALATAPIDSPLGVLEPGQVAGRKFHWEALVDGEPVVRVTVNWLMGEENLDPAWTFGPG
                     GQRYEMEVRGNPDITIAVHGFHSEIGGEGPEYGIVGTAAHCVNSVPAVCAAEPGILTM
                     LDLPLISGKAAPGLR"
     gene            1386..1790
                     /locus_tag="VGP27_06015"
                     /pseudo
     CDS             1386..1790
                     /locus_tag="VGP27_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911469.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
     gene            1965..>2407
                     /locus_tag="VGP27_06020"
     CDS             1965..>2407
                     /locus_tag="VGP27_06020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7850905.1"
                     /translation="MSGNEGDSRVGTEFGRYRLRRLLGKGGMGEVYEAEDTEKDRIVA
                     LKLLPQAMSHDPVFRKRLQREAHSAGRLLEPHVVPIHDYGEIDGVMFVDMRMIDGTDL
                     RKVLKNYGPMTPARAVAIVRQIASALDAAHESGIMHRDVKPENILL"
BASE COUNT          405 a          825 c          785 g          392 t
ORIGIN      
        1 tgccgccgcc gaccagcacg acggtgcgcg actggccgac cgcatcccag tcgccggtct
       61 ggttgcgtgt ctccttgatc ggcgggatcg ggccgtacca acgccggaac agctcgggca
      121 gcggcatcgg gaccatgtcg ctgaacacac actgctggcc catcgctatg gtccgggact
      181 gatcgaccac caacggcttc gtcatgaccc gagattagcg cagcccggga gccgccttgc
      241 cgctgatcag cgggaggtcg agcatggtga ggatgcccgg ctcggccgcg cagaccgcgg
      301 gcaccgagtt cacacagtgc gccgctgtac cgacgatgcc gtattccgga ccctcaccgc
      361 cgatctcgga gtggaagccg tggacggcga tggtgatgtc cggattgcca cggacctcca
      421 tctcgtatcg ctggccaccg ggcccgaatg tccatgcggg atcaaggttt tcctcaccca
      481 tcagccagtt caccgtcacg cgcacgacgg gctccccgtc gacgagtgcc tcccagtgga
      541 acttgcggcc cgcgacctgg ccgggctcga ggacgcccag cggcgagtcg atcggcgcgg
      601 tggccagcgc aacctcctgc gtcgcccgca ctttcgggtc ggcgttgaac cccatcttgg
      661 cgacgaccat cttcacggcc tggatgaaac cgctgtcgag catcttctgc atcggcccgg
      721 tcagcgcctt ctcggggacc tctccgaagc ccatgacgtg ccggagtaca tcgggtgcgt
      781 cgtagcttcg cagatcggaa aactcctctg cgcgaacgaa agtcacgccc gtcgagaatg
      841 cggagaacag cagcggaaac ttctcgctga tgccgccggg cgccatgcct gtgccgtgca
      901 gggtggcgtc tccttccagt gccgcttcac gcatcggcgc ggcgctgcgc tcgctggggt
      961 agagccagcc gaccggggtg acgacgttct tgcccgaccg cagcagcgcg gccacctcgt
     1021 cgggatcggc catcagcggc gcatagatca ccgcgtcggc gtccagggcg acgatgtcgt
     1081 cgatgctgtt cgtcgcggtg acgccgagcg gctcggttcc gatcaggtcg ccgacgtcgc
     1141 ggcccgcctt gtccgccgag tgcacccagc agccgacaag ctcgagttcc gggtgctcca
     1201 gcacgccctt gatcgccgcg acaccgacgc cgccggtggc ccattgcacg acccgaaggc
     1261 ccatgtcgtt cctcccagga aagtcgcaaa actagaacac gttctactca ggtgtacacc
     1321 acttcccgcc gaggcacgtc acaattgcgg tgatggccga gaagggtgcc gagctcggcg
     1381 tgccggcggt gctcgccatg ctgctgatca atcgcaaccg gcccgtcgca accgatgcgt
     1441 tgatcggggc ggtccgggag cggttcccgc ccccggaccc acgcgccagc cgtgtccaac
     1501 atccgcaagc tgctgggccg tatcggcgtc gactcccgta ccgcgctggc gagcgctccg
     1561 ccggggtatc ggttgagcgt gcccgaagat gactgtgaca taggttgatt cgcactcggt
     1621 aaagaccgcg ggcgtgcagg ctgcggccgc gggaacgttc gcacaggccg gcaaggccct
     1681 ttcggacggc gctcccagaa tggcgcgggc cggtgctggg agacttgcgc gacttccagt
     1741 tcgtcgacgc gttcgccacc gcgctcatcg aagacaagat gctcgcgcac aacgcgcaca
     1801 ccgcgctcgc cgcgtcacgc tctgcgacgg agaccgcatc cgcatctgcg accacccgtt
     1861 cgtcctcgaa gttcagcccc cagaggtgta gtcctgtttg cggtgtgacg cacgattcgg
     1921 ggcattaggg tgtgttcgct ggcaaatgcg acaaggagcg accgatgagc ggcaacgaag
     1981 gcgactcgcg ggtgggcacc gagttcggcc gctaccggct gcgtcgactg ctcggcaagg
     2041 gcggcatggg cgaggtctat gaggcggagg acaccgaaaa ggaccgcatc gtggcgctga
     2101 agctgttgcc gcaggcgatg tcccacgatc ccgtgttccg caagcggctg cagcgggaag
     2161 cgcactcggc gggccgactg ctggaacccc acgtcgttcc gatccacgac tacggcgaga
     2221 tcgacggcgt gatgttcgtc gacatgcgca tgatcgacgg caccgacctg cgcaaggtcc
     2281 tgaagaacta cggtccgatg acgcctgccc gggccgtggc gatcgtgcgc cagatcgcgt
     2341 cggcgctcga cgccgcccac gaatccggca tcatgcaccg cgacgtcaag cccgagaaca
     2401 tcctgct
//
LOCUS       DASYLX010000246         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1326362,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000246 DASYLX010000000
VERSION     DASYLX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326362"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGP27_06025"
     CDS             complement(<1..552)
                     /locus_tag="VGP27_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850906.1"
                     /translation="MSLAHAPKEIDGHGLLTGKVVVVTAAAGTGIGAAVARRALAEGA
                     DVVVSDHHERRLGETRDQLAGLGLGRVESVVCDVTSTAQVDALIASTTARMGRLDVLV
                     NNAGLGGQTPVVDMTDDEWDRVLNVTLTSVMRATRAALRYFRDAEHGGVIVNNASVLG
                     WRAQHSQSHYAAAKAGVMALTRCS"
     gene            complement(578..1759)
                     /locus_tag="VGP27_06030"
     CDS             complement(578..1759)
                     /locus_tag="VGP27_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850907.1"
                     /translation="MIEVQEFRAEVRDWLADNLVGEFAALKGLGGPGREDEAFEARLA
                     WNKHLAAAGLTCLGWPEEHGGRGLSVAHRVAFYEEYAKADAPDKVNHFGEELLGPTLI
                     AYGTPEQQQRFLPKILEVTELWCQGYSEPGAGSDLANVSTTAELDGDQWVINGQKVWT
                     SMAHLSQWCFVVARSEKGSQRHAGLSYLLVPLDQPGVEVRPIVQLTGTADFNEVFFDD
                     ARTDADLVVGTPGDGWRVAMGTLTFERGVSTLGQQIRYARELSNLAEVAKQTGSVDDP
                     LIRERLTRSWVGLQTMRSYALATMDVEQRGSRTAGDIGPGQDNVSKLLWANWHRDLGE
                     LAMDVVGKAGLVTDDGKFDQWQHLYLFTRADTIYGGSNEIQRNIIAERVLGLPREVKG
                     R"
     gene            1807..>2795
                     /locus_tag="VGP27_06035"
     CDS             1807..>2795
                     /locus_tag="VGP27_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7850908.1"
                     /translation="MTPVLRTIPQVLDRIADQFADHDALVTDAGGAGNPAGARRLTFV
                     QLRDEVRTAAAAMIDLGVAAGDRVAIWSPNTWHWVVACLATHYAGGVVVPLNTRYTAG
                     EAADVLARTGAPLLVAAGEFLGADKTAGLDRAALPDLRHIVRVPVDTDDGTWDEFVAR
                     GSDLDAVERRAAAVKPDDVSDILFTSGTTGRSKGALCAHRQSLDAPAAWAACGQLTSS
                     DRYLCINPFFHNFGYKAGILACLQTGATLIPQLTFDPEKAMAAVAEHRITVLPGPPTI
                     YQTLLDHPRRGDYDLSSLRFAVTGAATIPVVLIERMQSELDIDIVLTAYGLTEA"
BASE COUNT          453 a          984 c          890 g          468 t
ORIGIN      
        1 gctgcaacgg gtgagcgcca ttacgcctgc cttggctgcg gcgtagtgcg actgcgaatg
       61 ctgggcccgc caaccgagca cgctggcgtt gttgacgatc acgccgccgt gctcggcgtc
      121 gcggaagtac cgcagtgcgg cccgtgttgc ccgcatcacc gacgtcagcg tgacgttgag
      181 gacccggtcc cattcgtcgt cggtcatatc gacgaccgga gtctgaccgc cgaggccggc
      241 gttgttgacc agcacgtcga ggcggcccat ccttgcggtc gtcgacgcga tcagcgcgtc
      301 cacctgagcg gtcgacgtca catcgcacac cacgctctcg acccgaccca ggccgagccc
      361 ggccagctgg tccctggtct cgcccaaccg ccgttcgtgg tgatcggaga cgaccacgtc
      421 ggcgccctcg gccagcgcgc ggcgcgcgac cgccgccccg atgccggtgc cggccgccgc
      481 ggtcaccacc acgaccttgc ccgtcaaaag tccatgcccg tcaatctctt tcggagcgtg
      541 tgcgagactc ataccactct cctcttcgcg cgggcgctca tcggcccttc acctcacgcg
      601 gtaggccgag cacacgctcg gcgatgatgt tgcgctggat ttcgttggac ccgccgtaga
      661 tggtgtctgc acgggtgaac aggtaaagat gttgccactg atcgaatttg ccgtcatcgg
      721 ttaccaggcc ggccttcccg acgacgtcca tcgccagttc gccgagatcg cgatgccagt
      781 tggcccacag cagcttggac acattgtcct gacccggccc gatgtcgccg gcggtgcggc
      841 taccgcgctg ctccacgtcc atggtcgcca atgcgtagga acgcatcgtc tgcaggccga
      901 cccatgatcg agtcagccgc tcacggatca gcgggtcgtc gacgctgccg gtctgcttgg
      961 ccacctctgc gagattggaa agctcgcgcg cgtaacggat ctgctgaccg agcgtcgaaa
     1021 cgccgcgctc gaatgtcagt gtgcccattg cgacccgcca gccgtcgccc ggggtgccga
     1081 ccacgaggtc ggcgtcggtg cgggcgtcgt cgaagaacac ttcgttgaaa tccgcggtgc
     1141 cggtcaactg gacgatcggg cggacctcga cgccgggctg atcgagcggc acgagtagat
     1201 aggacagtcc cgcatggcgt tgggaaccct tctcgctgcg ggcgaccacg aaacaccact
     1261 gcgacagatg ggccatcgac gtccagacct tctggccgtt gatcacccac tggtcgccgt
     1321 cgagttcggc ggtggtcgac acgttcgcca gatcgctccc ggctccgggc tccgaataac
     1381 cctgacacca cagttcggtg acctcgagga tcttcggaag gaaccgctgc tgctgctccg
     1441 gtgtgccgta agcgatcagc gtcgggccga gcagttcctc gccgaagtgg ttcaccttgt
     1501 cgggtgcgtc ggccttggcg tactcctcat agaaggcgac gcggtgcgcg accgaaaggc
     1561 cgcggccacc gtgctcctcg ggccaaccca gacacgtcag gccggcagcc gcgaggtgct
     1621 tgttccacgc cagccgtgcc tcgaatgcct catcctcgcg accgggtccg cccaggccct
     1681 tgagcgcggc gaattcaccg acgagattgt cggcgagcca gtcgcggacc tcggcccgga
     1741 actcctggac ctctatcacc cctgtaggct aacctaccaa gcacttgctt tgttagagga
     1801 gcatcgatga cgcccgttct gaggaccatt ccgcaggtcc tggaccggat tgccgaccaa
     1861 ttcgccgacc atgacgcgct ggtcaccgac gccggcggtg ccggcaatcc agcaggagct
     1921 cggcggctga ccttcgtgca gttgcgcgac gaggtccgaa cggccgccgc ggcgatgatc
     1981 gaccttgggg tcgccgccgg ggaccgcgtc gcgatctggt cgccgaacac ctggcactgg
     2041 gtagtcgcat gcctggcgac ccactatgcg ggcggcgtgg tcgtccccct caacacgcgc
     2101 tacaccgcgg gagaggccgc cgacgtgctg gcccgcaccg gagcgccgct gctggttgcg
     2161 gcaggtgaat tcctcggcgc cgacaagacc gcgggcctgg accgggctgc gctgcccgat
     2221 ctgcggcaca tcgtgcgcgt cccggtcgac accgatgacg gcacgtggga cgagttcgtc
     2281 gcccgtggct cggatctgga cgcagtcgag cgccgcgcag ccgccgtgaa gccggacgac
     2341 gtctcagaca tccttttcac gtccggcacc accggccgca gcaagggtgc gctgtgcgca
     2401 caccggcagt cgctcgacgc gcctgcggca tgggcggcgt gcggacagct caccagctcc
     2461 gaccgctacc tgtgtatcaa cccgttcttc cacaacttcg gctacaaggc cggcatcctg
     2521 gcctgcctgc agaccggggc aacactgatc ccgcagctga cgttcgaccc ggagaaggcg
     2581 atggccgcag tggccgaaca ccggatcacc gtgcttcccg gaccgccgac gatctaccag
     2641 accctgctcg accaccccag gcgcggcgac tacgacctca gttcactgcg attcgcggtc
     2701 accggcgcgg cgacgatccc ggtggtgctg atcgagcgca tgcagtcgga actcgacatc
     2761 gacatcgtgc tgacggcata cgggctgacc gaggc
//
LOCUS       DASYLX010000247        16684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3191093,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000247 DASYLX010000000
VERSION     DASYLX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191093"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VGP27_06040"
     CDS             <1..1327
                     /locus_tag="VGP27_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7850909.1"
                     /translation="IAIGTAADVDKAVKAARKAFATWSVSTREERLDLLQAIAADYQK
                     RSEDLADAVHEEMGAPPSLAAGPQVMMGMGHLMGAIDALKNFSFEEQRGSTLILKEPI
                     GVCGLITPWNWPINQIACKVFPALATGCTMVLKPSEVAPYSGQIFTEVMDSAGVPAGV
                     YNMVFGDGPGVGMALSSHPDIDLVSFTGSTRAGVDVAKNAAATVKRVTQELGGKSPNI
                     VIDDEKFAESVTAGVSTMMVNTGQSCNAPSRMLVPRDRIAEAAEIARGVAEAVSVGDP
                     DDKRAIGPVASKAQFDKVQGLIQKGIDEGATVAAGGPGRPDGLDTGYYVRPTVFSDVN
                     NDMTIAREEIFGPVLCILPYDDLDQAVDIANDTDYGLAGYVSAADLDAARTLARRIRA
                     GQVAINHAFDLNTPFGGYKRSGNGREWSEFGFEEYLEIKGTLGYAPQTS"
     gene            complement(1331..1801)
                     /locus_tag="VGP27_06045"
     CDS             complement(1331..1801)
                     /locus_tag="VGP27_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7850910.1"
                     /translation="MHELPVELTVNGRTYRHVVEPRLTLADFLREKCGLTGTHLGCEH
                     GACGACTVLLDGQAVRACLIFAVQVDGQEVTTVEGIAGDDGTLSPVQSALRECHGLQC
                     GFCTPGFVTSITALLRDNPQPTDEEIREGLSGNFCRCTGYQGIINAVHRAAQTS"
     gene            complement(1794..2690)
                     /locus_tag="VGP27_06050"
     CDS             complement(1794..2690)
                     /locus_tag="VGP27_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7850911.1"
                     /translation="MKAAAFAYHRPDTVAEAAQMLAEFGDGAKILAGGQSLVPMLAMR
                     LTYFENLIDISRVEELKNIELRDDGLSVAAGTSHALVGMDDEVAESVPLLTLSTPYIG
                     HFQIRTRGTLGGAIAHADPAAEYAAVALTLDATIEAASARERREVPAAEFFTGLWETS
                     LAPDEIVTAVRFPVWSGRCGFAVEEFARRHGDFAIAGATVAVELDDDDRVARCGIGLL
                     GLGSTPLRGSAAEDAVAGAPAGTVTAEEVGRLAMARLSDIPADLQGSADYRSRVGAAM
                     VARAWAAAVRQAHTSETEAIHA"
     gene            complement(2687..3388)
                     /locus_tag="VGP27_06055"
     CDS             complement(2687..3388)
                     /locus_tag="VGP27_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850912.1"
                     /translation="MELNNEFRVAVPAAKTWQVLTDVERVAPCIPGAQLLSVDGDDFT
                     GVVKVKVGPITVSYKGDASFQEKDEAAQRVVIKATGKETRGSGNAAAVVTAQLKDDGT
                     HGTIVSITTDLTVSGKAAQFGRGVLADVSTNLIGQFARNLEADLLGDTATSAAASTPV
                     AAATAAATASDGVDSVDLLKVVAVPMAKRYAPAIAAVAAGVTVGFLLGRRKRAHPAAD
                     LADELQTALARLLKS"
     gene            complement(3391..5748)
                     /locus_tag="VGP27_06060"
     CDS             complement(3391..5748)
                     /locus_tag="VGP27_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7850913.1"
                     /translation="MTETVATRYAGTRVPRVEDTRLLTGHGTFVDDVSRPGMLHACFV
                     RSPFARARINGIDTSAARALSGVQAVFLAGDLNPDVEEAWHAVAGKDIADTPRPPLAE
                     GEAKFVGDPVALVIAESRYVAEDAVELVDVDYDPLPAVADFTQAVRLAADSEVVVHDA
                     YPDNVAGGMGGAPADEETFSSAAHVASAHVYQQIHAPVPIETRGLVAEWEGSSRELTM
                     WASTQTPHELRAFAARLLGIPAQNVRVIMRDTGGGFGQKVVPMREDMCVMLAARKVSG
                     ALKWIEDRRENLMSAGQARHVDGDVRMAFDDDGNILAADIDFVQDVGAYPTPYPVLTT
                     AAIGMFFPGPYRVPKASFIYKTVFSNTAGLAAYRGPWQYETLTREILLDIGARKMGMD
                     PVELRRRNILRADEMPYFNPNGMPYEHVAPADTFEQAVKMLDHEGFRREQRDALEEGR
                     YVGLGFSAYIEPTGAATGHLGTEGATIRMEPTGKINVYVNGGSTGNSIETTVVQLTAD
                     ALGANIEDVSTIQGDTAVTPYGAGTQGSRSGPMTAGAVDAAGAILRKQIVAMAAHRLG
                     VDESDIELANSRAGVRDDPEKGVSFADLAFRSYYEPSQLPPGMAATLEATARFTSQTM
                     IHWANATHACTCEIDVETGHVTLTRYIVSEDVGPMINPNVVEGQIAGGTVQGIGGALL
                     ENMVYDDSGNPLSSTFVDYLLPTATEVPVIEYGHVEIPGAGVGGYKGCGEGGAIGSTP
                     AVINAINDALAPLGVTLTRLPATPAAIVELIEKAKADTGAARKDH"
     gene            5934..6401
                     /locus_tag="VGP27_06065"
     CDS             5934..6401
                     /locus_tag="VGP27_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7850914.1"
                     /translation="MRRWITRIERTLCEDVASPPDEVRAFFVDLDNIKLVHPLVVSVR
                     SVDRSETADGYVQTYRVGDRIPIGPFRLRTSYVARLYVPLTGDVLSEARQFPRVCLRS
                     TVAFEPTEVGTRVTERIRIEAPRPLASVTTRKAVEAHTEMLAGIRRRYERTPS"
     gene            complement(6410..6835)
                     /locus_tag="VGP27_06070"
     CDS             complement(6410..6835)
                     /locus_tag="VGP27_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850915.1"
                     /translation="MIREAVIAAALACAAVGSAQVAAADPNDNLYFDKPGRYDSDVPF
                     MNYEARLSAPCDNFEINTFGRGPGGEPLQCHWIPNQWPPVYTGFWVISPEIHGVQQIG
                     TPCPSDQAAAQAPDGRPLLCLGEQGWQPGFFTGSGFWPT"
     gene            6925..7101
                     /locus_tag="VGP27_06075"
     CDS             6925..7101
                     /locus_tag="VGP27_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850916.1"
                     /translation="MAPGDDDKSEEDVQDDDKVAGSRAGKHGEDGSYVGETSNDDAID
                     AGETGAEARAEQKG"
     gene            7184..7747
                     /locus_tag="VGP27_06080"
     CDS             7184..7747
                     /locus_tag="VGP27_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850917.1"
                     /translation="MDGFEYVSYEEFGRRFYEIAVTEERVGDAIAAIAGDEFEMGPMG
                     QGPGKIAKVTAKVRIQEPRVTRAVGEMITFAIRIPLEIDMVIDLKIDKPKFMVFGEIS
                     LNAVARAAEPLLLILDVQKPRPSDIAIHVTSKSLRAELLRIVGGVDAEIRRFISAHVA
                     GEIDSPASVKAKVIDVADRLDAAWDGV"
     gene            complement(7750..8631)
                     /locus_tag="VGP27_06085"
     CDS             complement(7750..8631)
                     /locus_tag="VGP27_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEV7850918.1"
                     /translation="MPKSPDASGPLKPHFEDVQAHYDLSDDFFRLFLDPTQTYSCAYF
                     ERDDMTLEEAQIAKIDLALGKLGLEPGMTLLDIGCGWGATMRRAIERYDVNVVGLTLS
                     KNQAAHVQKAFDRMGSPRTKRVLLQGWEQFHEPVDRIVSIGAFEHFGHDRYQDYFRMA
                     YDVLPEGGVMLLHTIVGENPARAKELGIPLTFELARFIKFIMTEIFPGGRLPSVPMVE
                     ERAAEGGFTLTRKQELQPHYARTLDLWAAALEAHKDEAIAIQSEEVYDRYMKYLTGCA
                     KMFRDRQVDVVQFTLAK"
     gene            8694..9068
                     /locus_tag="VGP27_06090"
     CDS             8694..9068
                     /locus_tag="VGP27_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7850919.1"
                     /translation="MRRLRRPVRLITVFTGLAAATSVLAAPAQAEPDTDAFLSAVTSA
                     GLGGTTDPSNLVTVGQAVCPMLSDPGQNAADVAAKVADTTGMSLGGANMFTGLAISFL
                     CPRVMESVAGGQSPIPLGLLGF"
     gene            complement(9065..9841)
                     /locus_tag="VGP27_06095"
     CDS             complement(9065..9841)
                     /locus_tag="VGP27_06095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV7850920.1"
                     /translation="MVHGTYTRYVALGDSQTEGLWDGDDSDGIVGFADRLAAMLDELS
                     PGLQYANLAIRGRRIRDVLIDQLPLALTMRPDLVSVCVGMNDITRPGLLFDRALADLD
                     LLYERLAQSGATIVTTTFPDIARIVPIGKILTARMSRTNRAIVAAADRYGFKLVDLYG
                     APSMTEPDVWSDDRVHGSTKGHMLFAAAAAEALNLPGSNHDWAYAGAAAARSTMRSRA
                     HSQVLWTRNMLAPWLWHHALGISTGDGRAPKRPRLESVAG"
     gene            complement(9859..10668)
                     /locus_tag="VGP27_06100"
     CDS             complement(9859..10668)
                     /locus_tag="VGP27_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HEV7850921.1"
                     /translation="MTRSRPLRRWDHFRPLDMLGSLWSTAAVAPVSTGAAAAYRTLFM
                     TLRRLVVGRELSVRLDEGDITLTVTEFDSRLDVRGLAAGQLSDVWLAARDIRWDRNRL
                     DRATAVLHNVHLRPSAPPVLVAAPVDLTVELPADALDDLFRLVAPRLAGEIGPDGIAR
                     LRLARRPSLGHLEVDAVLDGSTLWLKPRGLVVRRRRLGLPKRTPAHAVQLPELPHGLR
                     LTDIRFEPDKLCLSGVLQEWRIDVPRTRIEDVITQLSAVGRPLNLTRFGRG"
     gene            complement(10715..11317)
                     /locus_tag="VGP27_06105"
     CDS             complement(10715..11317)
                     /locus_tag="VGP27_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850922.1"
                     /translation="MPASEPSSLRAEQVAQTRAALVAAGRRLFGENGFRGTPVEDLAR
                     EARVTTGALYHHFPTKTALFEAVFVQAHMELMTSATEAAMGAEDGLDELALGFDAFLD
                     GVLQPDVQRIVVLDGPAVLGLARYTELDEQYAYAVIVGSLKEAAKAGAVDIDDPETAT
                     RLLLGALTRGAMLIANSPDPVKTRHAVANSMRALLNSFAP"
     gene            11407..12246
                     /locus_tag="VGP27_06110"
     CDS             11407..12246
                     /locus_tag="VGP27_06110"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850923.1"
                     /translation="MRTTGPAEDTRRAHGATRDALGAAVRRTAELFQRVDDADAPVPG
                     LEWTVAETAAHMVGELRDYGQALTRHTNGYMTHANRAQESPSRMSAVVNARQLTEIPE
                     RDMNRLAGLLEDAALAYLTAAAAADETAAIPAANGLVLNPSTMTSLLLGEQVIHGLDI
                     ARAVGARWDIRGHDALLIIPGALTVAPHYLRPSAAAARVSFELRIRGANTYRMAVDRG
                     TAVVTGAADRSDCVITADPVAFLLLGFGRVSQWSPILRGKLRAGGRKPWSAMKFATLL
                     SSP"
     gene            12264..13583
                     /locus_tag="VGP27_06115"
     CDS             12264..13583
                     /locus_tag="VGP27_06115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850924.1"
                     /translation="MDSKTSVVIVGSGFTGFECARRLSRQLRKHDAAVDISIISPVDY
                     MLYTPLLPDVAGGVVDARFVTVPLANTLHGVRAVRGRVDSVDLDGHTVTYTDPEQRSR
                     NMSWDRLVVTPGSVTRLFDIPGLAEHARGLKSTAEAVYLRDHLLEQLELANIDDDEAR
                     IQARRTVVVVGASYSGTELIVQLQALADTAAKQTGFDPAGMKFVLLDMAEQVMPEVGE
                     KLGKGVQRVLHQRGIDVRLGLTLKEVHADHVVLSDETRIDTHTVAWVTGVTGAPLIET
                     LGLPTERGRLKVQANLQVPGHPDVFAAGDAAAVPDLTQQGEITPPTAQHAIRQGKVLG
                     RNVAASLGYGEAKDYRHRNMGLVVDLGPHYAVANPLNIQLSGYPAKFVTRMYHLYAMP
                     RFVNRWAVSLSYLTDVFFDRSIVSVGLSSQDDASFSTSEGIPLPKFD"
     gene            13619..13810
                     /locus_tag="VGP27_06120"
     CDS             13619..13810
                     /locus_tag="VGP27_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850925.1"
                     /translation="MATYRVLNPKGDVVDTKDIESAQDAHAWFVDAKADNSELGWRME
                     VEADDGHWAFFDDTEGDRK"
     gene            complement(13811..14026)
                     /locus_tag="VGP27_06125"
     CDS             complement(13811..14026)
                     /locus_tag="VGP27_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850926.1"
                     /translation="MGDFEPRWRLDLDGDGTVTFDRDTQTITKIKTGESCCLGDYPTF
                     SVEEPRYLRVRSSHAPPIGKWYVTGED"
     gene            complement(14026..14397)
                     /locus_tag="VGP27_06130"
     CDS             complement(14026..14397)
                     /locus_tag="VGP27_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850927.1"
                     /translation="MNRAAGGLAAGALMAGLVVAAPANADPPSYGSNGVFSVGAEPRQ
                     GWSTASIPPGRYRVDQSPSMYPYQSPPGMWLRCNSFPCAGNYPQNIIGTGEALRDQPT
                     FVDILPSDVAVSLLNVTLTVA"
     gene            complement(14402..15253)
                     /locus_tag="VGP27_06135"
     CDS             complement(14402..15253)
                     /locus_tag="VGP27_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV7850928.1"
                     /translation="MNRRNLMMLTGAAALAAAITAPQAAADPLAPGQPENPGAPPPNA
                     NPAPVDYILVDDFDGPAGAPPDFGRWTVVGWNEPVNPPILGLFRDDRRNVSLDGNGNL
                     VLRATREGDTYFSGRVESKMKIGIGHTWEARMKLNCLVPGCWPAYWLVNREPTPDGRG
                     PLPDGEVDILEWYGNGEWAPGTAVHARSDGKTWKENRWPVDGDWHTYRVRWDDAGFRF
                     WRDDVNGAPYFTVPSVPIDGVWPFNAPDYLLYTVFNLAVGGPGGGDPAFGPFQSPEML
                     IDYVRVW"
     gene            15313..15783
                     /locus_tag="VGP27_06140"
     CDS             15313..15783
                     /locus_tag="VGP27_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7850929.1"
                     /translation="MPRLSAGLLLYRVVDGSVEVLIGHPGGPFWAKKDDGAWSIPKGE
                     YTDGEDPWAAARREFEEELGKPPPDGPRIDFPPLKQPSGKIITAFAVRGDLDLEGTVS
                     NTFTLEWPKGSGNVREYPEIDRADWFCVPAARSKLLKGQRLLLDRLVDVLANAE"
     gene            complement(15875..16219)
                     /locus_tag="VGP27_06145"
                     /pseudo
     CDS             complement(15875..16219)
                     /locus_tag="VGP27_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(16225..>16684)
                     /locus_tag="VGP27_06150"
     CDS             complement(16225..>16684)
                     /locus_tag="VGP27_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV7850930.1"
                     /translation="ALVLIQFREIEKLLNDDYTPSPRPPATSPRAEAPPALPPPPSES
                     TPSPTTTTTASAAPAAAVVGADCSPVGDTATTANGNTVYCSTLEGTGASIWSVTQGVV
                     PAPTITVTTQATDEPLPVAEEAPVRVCMQETGKTRRQCREDIRAGNQAGS"
BASE COUNT         2773 a         5490 c         5625 g         2796 t
ORIGIN      
        1 gatcgcgatc ggcacagcgg cagacgtcga caaggcggtc aaggcggcgc gcaaggcatt
       61 tgcgacgtgg tcggtgagca cccgcgagga gcggctggac ctgctacagg cgatcgccgc
      121 ggactaccag aagcgctccg aggatctcgc cgatgccgtg cacgaggaga tgggcgcacc
      181 gccgtcgctg gcggccgggc ctcaggtgat gatggggatg ggccacctga tgggcgcgat
      241 cgacgcgttg aagaacttct cgttcgagga gcagcgcggg tcgacgctga ttctcaagga
      301 gccgatcggc gtctgcggcc tgatcacgcc atggaactgg cccatcaacc agatcgcctg
      361 caaggtgttc ccggccctgg ccaccggctg cacgatggtg ctgaagccgt cggaggtggc
      421 gccctattcg ggccagatct tcacggaggt gatggattcg gcgggggtgc ctgcgggcgt
      481 ctacaacatg gtcttcggcg acggccccgg cgtcggaatg gcactctcca gccaccccga
      541 tatcgacctg gtgtcgttca ccggttcgac cagggccggc gtcgacgtcg cgaagaacgc
      601 ggctgccacc gtcaagcggg tgacccagga actaggcggt aagagcccga acatcgtcat
      661 cgacgacgag aagttcgccg aaagcgtcac cgcgggcgtg tccacgatga tggtcaacac
      721 cggccagagc tgtaatgcgc cctcgcgcat gctcgttccc cgcgaccgca tcgccgaagc
      781 cgccgagatc gcacgcggcg ttgctgaagc ggtttcggtc ggcgaccccg acgacaaacg
      841 ggcgatcggc ccggtcgcct ccaaggctca gttcgacaag gtgcagggcc tgatccagaa
      901 gggcatcgac gagggcgcca cggtggcggc cggcggaccg ggacggcccg acggtctcga
      961 caccggttac tacgtcaggc cgacggtgtt ctcggatgtc aacaatgaca tgaccatcgc
     1021 ccgtgaagag atcttcggcc cggtgctgtg catcctgccc tacgacgacc tcgatcaggc
     1081 cgtcgacatc gcgaacgaca cggattacgg tctggcaggc tatgtttcgg ccgcggacct
     1141 cgatgccgcc cgcacgctgg cacgccggat ccgggccggc caggtcgcca tcaaccacgc
     1201 cttcgacctg aacactccgt tcggcggcta caagcgcagc ggtaacggtc gcgagtggag
     1261 cgagttcggt ttcgaggaat acctcgagat caagggcacg ctggggtacg cgccccagac
     1321 gtcctagccg ctaggacgtc tgggcggcgc ggtgcaccgc gttgatgatg ccctggtaac
     1381 cggtgcaccg gcagaagttg ccggagaggc cttcgcggat ctcctcgtcg gtgggttgcg
     1441 ggttgtcgcg cagcagcgcg gtgatcgaag tgacgaaacc cggtgtgcag aaaccgcatt
     1501 gcagcccgtg gcactcgcgc agcgccgact gcacgggcga cagcgtgccg tcgtcgcccg
     1561 cgatgccttc gacggtggtg acctcctggc cgtccacctg gacggcgaag atcaggcacg
     1621 cccggacggc ctgaccgtcc agcagcaccg tgcacgcgcc gcaggcgcca tgttcgcagc
     1681 cgaggtgggt gccggtgagg ccgcacttct cgcgcaggaa gtcggcgagc gtcagacggg
     1741 gctcgaccac gtgccggtac gtgcggccgt tgacggtcag ctcgacgggc agctcatgca
     1801 tggatcgcct ccgtttcgga cgtgtgtgcc tgccgcacgg ccgcagccca tgcgcgcgcc
     1861 accatcgcgg ctccgacacg ggaccggtag tcggccgaac cctggaggtc ggccggaatg
     1921 tcggacagtc gggccatcgc gagccttccg acctcctcgg cggtgacggt gccggcgggc
     1981 gcgccggcga ccgcgtcctc ggccgccgag ccgcgcagtg gcgtcgaccc gaggccgagc
     2041 agtccgatgc cgcatcgcgc gacccgatca tcatcgtcga gctcgaccgc aaccgttgcg
     2101 ccggcaatcg cgaaatcgcc gtgccgacgg gcgaattcct cgacggcgaa gccgcaccgt
     2161 ccgctccata ccggaaaccg cacggcggtg acgatctcgt cgggcgccag ggaggtctcc
     2221 cacagcccgg tgaagaactc cgcggccggg acctcgcgtc gttcgcgtgc cgacgccgcc
     2281 tcgatcgtcg cgtcgagcgt gagcgcgacc gccgcgtact cggcggcggg gtcggcgtgc
     2341 gcgatcgccc cgcccagcgt gcccctggtc cggatctgga agtgaccgat gtagggcgtc
     2401 gacagggtca gcagcggcac cgactcggcc acctcgtcgt ccatgcccac caacgcgtgc
     2461 gaggtcccgg ccgcaaccga caatccgtca tcacgcagtt cgatgttctt caattcctcc
     2521 acgcgcgaga tgtcgatcag gttctcgaag tacgtcagcc gcatggcgag catcggcacc
     2581 agactctgcc cgcccgccag gatcttggcg ccgtcaccga actcggcgag catctgggcg
     2641 gcctcggcca cggtgtcggg gcgatggtag gcgaaggcgg ccgccttcat gacttcagca
     2701 gccgagcgag agcggtctgc agctcatcgg cgaggtccgc ggcgggatgg gcgcgcttgc
     2761 gcctgcccag caggaagccg accgtcacgc ctgcggcaac cgcagcgatc gcgggcgcgt
     2821 agcgcttggc catcggcacc gcaacgactt tcagcagatc gaccgagtcc accccgtcag
     2881 aggcggtcgc tgcggccgtc gcggcggcga ccggcgtcga tgccgcagcc gacgtggcgg
     2941 tgtcgccgag cagatcagcc tccaggttcc tggcgaactg gccgatcagg ttcgtggaca
     3001 cgtcggccag cacgccgcgg ccgaactgcg ccgccttgcc ggagacggtc aggtctgtgg
     3061 tgatggaaac gatggtgccg tgggtaccgt cgtccttcaa ctgggcggtg acgactgcgg
     3121 cggcgttacc gctgcccctg gtctccttgc cggttgcctt gatgaccacc cgctgggctg
     3181 cctcgtcttt ctcctgaaac gacgcatcgc ccttgtagga caccgtaatc gggccgacct
     3241 tcaccttcac gacaccggtg aaatcgtcgc cgtcgaccga aaggagctgc gcgccgggga
     3301 tacacggcgc cacccgttcg acatctgtga ggacctgcca tgtcttcgcc gcgggcaccg
     3361 cgactcggaa ctcgttgttc agctccacct ctagtggtcc tttctcgctg cgcctgtgtc
     3421 ggccttcgcc ttttcgatga gctcgacgat cgcggcgggg gtggcgggca gccgggtcag
     3481 ggtcacgccc agcggcgcca acgcgtcgtt gatcgcgttg atgaccgcgg gcgtcgagcc
     3541 gatcgcaccg ccttccccgc agcccttgta tccgccgacg ccggcgccgg ggatttcgac
     3601 gtgcccgtac tcgatcacag gcacctcggt ggcggtgggc aacaggtagt cgacgaaggt
     3661 cgacgagagc gggttgccgc tgtcgtcgta gaccatgttc tccagcaacg cgccgccgat
     3721 gccctgcacc gtgccgcccg cgatctgacc ctcgacgaca ttggggttga tcatcgggcc
     3781 gacgtcctcg ctgacgatgt agcgggtcag cgtgacgtga ccggtctcga cgtcgatctc
     3841 gcaggtgcac gcgtgggttg cgttggccca gtgaatcatc gtctgcgagg tgaaccgcgc
     3901 ggtcgcctcc agagtggccg ccatccccgg cggcagctgc gatggttcgt agtaggagcg
     3961 gaaggcgaga tcggcgaagc tgacgccctt ctccgggtcg tcgcggacac ccgcccgcga
     4021 gttcgcgagc tcgatgtcgg actcgtcgac gccgagccga tgggctgcca tcgcgacgat
     4081 ctgcttgcgc agaatcgcgc cggccgcgtc gacggcacct gcggtcatgg gtccgcttcg
     4141 actgccttgc gtgcccgccc cgtacggcgt cacggcggtg tcgccctgga tcgtcgagac
     4201 gtcttcgatg ttcgcgccaa gcgcgtcggc ggtcagctgg accaccgtcg tctcgatgct
     4261 gtttccggtc gacccgccgt tgacgtagac gttgatcttg ccggtcggct ccatccggat
     4321 cgtcgcgcct tcggtgccga ggtgtccggt cgcggcgccg gtcggctcga tgtaggccga
     4381 gaacccgagc ccgacgtagc ggccctcctc gagcgcgtcc ctctgctccc tgcggaagcc
     4441 ctcgtggtcg agcatcttca cggcctgctc gaaggtgtcc gcaggcgcga cgtgctcgta
     4501 gggcatgccg ttggggttga agtagggcat ctcatcggcg cgcaggatgt tgcgcctgcg
     4561 cagttcaacc gggtccatgc ccatcttgcg cgcgccgata tcgagaagta tctcgcgcgt
     4621 gagggtttcg tactgccatg gaccgcggta agcggcgagc cccgcggtgt tcgagaacac
     4681 ggtcttgtag atgaagcttg ccttcggcac ccggtacggg ccggggaaga acatgccgat
     4741 cgcggcggtg gtcagtaccg gatacggcgt cggatacgca ccgacgtcct gcacgaagtc
     4801 gatatcggcg gcgaggatgt tgccgtcgtc gtcgaacgcc atccgcacgt cgccgtcgac
     4861 gtggcgggcc tggccggccg acattaggtt ctcgcgccgg tcctcgatcc acttcagcgc
     4921 gccggacacc ttgcgggcgg ccagcatgac gcacatatct tcgcgcatcg ggacgacctt
     4981 ctggccgaac ccgccaccgg tgtcgcgcat gatgactcgc acgttttggg cgggaatgcc
     5041 gagcagccgc gcagcgaacg cacgtagttc gtggggcgtc tgcgtggagg cccacatcgt
     5101 caattcgcgc gaagacccct cccattcggc gaccaggccg cgcgtttcaa tcgggacagg
     5161 cgcatggatc tgttggtaca cgtgcgccga tgcgacatgg gcggccgacg agaacgtctc
     5221 ctcgtcggcc ggggccccgc ccatcccgcc cgcgacgttg tccgggtagg cgtcgtggac
     5281 gacgacctcg gagtcggcgg ccagccgaac agcctgcgtg aagtcggcga cggcgggcag
     5341 agggtcgtag tcgacgtcga ccagttcgac ggcgtcctcc gcgacgtagc gcgactcggc
     5401 gatgaccagc gcgaccggat cgccgacgaa tttcgcctcg ccttcggcca gcggcgggcg
     5461 cggcgtgtcg gcgatgtcct tgccggcgac agcgtgccat gcctcctcga cgtcggggtt
     5521 gagatccccg gcgaggaaca ccgcctgcac gccggaaagc gcccgcgccg cggaggtgtc
     5581 gatgccgttg atacgagccc tggcaaaagg actgcgaacg aaacaggcat gcagcatgcc
     5641 gggtcgcgag acatcgtcga cgaaggtgcc atgcccagtg agcaggcggg tgtcctctac
     5701 tcgaggaacc cgggtgcccg catacctggt ggcgacggtt tcggtcatgg caaacgctcc
     5761 aatccgccgt cgatgggccg ctacgacaag gtgctaagca cttgctcagc gagcctaccg
     5821 gatatcagat tctcgctgtt gaacatatgt cattctcgcg tgcgggccgg acagcattca
     5881 atgacctggc gaatgcggtc gtcgacgtcg accagccgat ctagattgtc atcgtgcggc
     5941 ggtggatcac ccggatcgag cgaactctct gcgaggacgt ggcaagcccg cccgacgagg
     6001 ttcgcgcctt cttcgtcgac ctcgacaaca tcaagctggt gcatcccctc gtggtttcgg
     6061 tgcgcagcgt cgaccgtagc gagacggccg acggttacgt gcagacctac cgggtgggtg
     6121 accggattcc catcgggccg tttcgcctgc gcacgagcta cgttgcgcga ctgtatgttc
     6181 cgctgacggg cgacgtgttg tccgaggcgc ggcaatttcc ccgagtctgc ctgcgcagca
     6241 ccgtggcctt cgaaccgacc gaggtgggca cccgcgtcac cgagcggatc cggatcgagg
     6301 cgccgcgacc gctggcgtcc gtgaccaccc gcaaagccgt cgaggcacac accgagatgc
     6361 tcgcaggcat tcggcgacgc tacgaacgaa ctccgagcta gctccgtgtc taggtgggcc
     6421 agaacccgga accggtgaag aagccgggct gccagccctg ctctccgagg cacagcaggg
     6481 gccgtccgtc gggggcctgc gcggcggcct gatcgctcgg gcacggggta ccgatctgct
     6541 gcacgccgtg aatctccggc gagatcaccc agaagccggt gtagaccggc ggccactggt
     6601 tgggaatcca gtggcactgc agcggctcgc cgcctggacc gcgaccgaac gtgttgatct
     6661 cgaagttgtc gcagggagcg gagaggcggg cctcgtagtt catgaacggc acgtcggagt
     6721 catagcggcc aggcttgtcg aaatacaggt tgtcgttcgg gtcggccgcg gcgacctgtg
     6781 cactgccgac tgcggcgcac gccaatgcgg cggcgatgac tgcttcgcgg atcattgtcc
     6841 ccaccttcct cgtccggaag cctatcggta gagctcgggt cgggtaagtg tttgggtccc
     6901 tccggtacgg gtagttaccg cgacatggcg ccaggcgatg atgacaagag cgaggaagac
     6961 gtccaggacg acgacaaggt ggcgggttcg cgggccggta agcacggcga agacggcagc
     7021 tatgtcggcg aaacgtccaa cgacgacgcc atcgatgcgg gtgagaccgg ggcagaggcg
     7081 cgcgccgaac agaagggctg attcgcccga ccctcgacag tggccggacc gctgcgtgga
     7141 catatgagca gtaatggccg cggacgtgag actgtagaag cctatggatg gcttcgagta
     7201 cgtgtcctat gaggaattcg gccgcaggtt ctatgagatc gcggtgaccg aggagcgcgt
     7261 cggcgacgcg atcgccgcga tcgcgggcga cgagttcgag atgggcccga tgggacaggg
     7321 gccgggcaag atcgcgaagg tgaccgccaa ggtccggatc caggagccta gggtcacccg
     7381 ggcggtcggc gagatgatca ccttcgcgat ccgcatcccc ttggagatcg acatggtcat
     7441 cgacctgaag atcgacaagc cgaagttcat ggtgttcggc gagatctcgc tgaacgccgt
     7501 cgcacgcgcc gccgaaccgc tgctgctcat cctcgatgtc cagaaaccgc gaccgtcgga
     7561 catcgccatt catgtcacct cgaaatccct gcgcgccgaa ctgttgcgga tcgtcggcgg
     7621 cgtcgacgcg gagatccggc gcttcatctc cgcgcacgtc gcgggggaga tcgacagccc
     7681 ggcgtcggtc aaggcgaagg tgatcgacgt cgccgaccgc ctcgacgccg catgggacgg
     7741 cgtgtagcgc tatttcgcga gcgtgaactg gacgacgtcg acctggcggt cgcggaacat
     7801 cttcgcgcaa ccggtcaggt acttcatgta gcggtcgtaa acctcttcgg actggatcgc
     7861 gatggcctcg tccttgtgcg cctcgagcgc ggcggcccac aggtcgagcg tgcgcgcgta
     7921 gtgcggctgc agctcctgct tgcgggtcag ggtgaacccg ccctctgccg cccgctcctc
     7981 gaccatcggc accgagggca gccgtccgcc ggggaagatc tcggtcatga tgaacttgat
     8041 gaagcgtgcc agctcgaacg tcaacggtat gccgagttct tttgcgcgcg cggggttctc
     8101 gccgacgatt gtgtgcagaa gcatgacacc gccttcaggt aacacgtcgt aggccattcg
     8161 gaagtagtcc tgatagcgat cgtgaccgaa gtgctcgaag gcgccgatgg acacgatgcg
     8221 gtccaccggc tcgtggaact gctcccaacc ctgcagcagc actcgcttgg tgcgcgggct
     8281 gcccatgcgg tcgaacgcct tctgaacgtg cgccgcctgg ttcttcgaca gggtcagccc
     8341 gacgacgttg acgtcgtagc gctcgatcgc ccgtcgcatg gtggcacccc agccgcagcc
     8401 gatgtcgagc agcgtcatgc cgggctcgag gccgagcttg cccagcgcga ggtcgatctt
     8461 ggcgatctgc gcttcctcaa gcgtcatgtc gtcgcgctcg aaatacgcgc agctgtaggt
     8521 ctgcgtcggg tcgaggaaca gccggaagaa gtcgtcgctg aggtcgtaat gcgcctggac
     8581 gtcctcgaaa tgcggcttga gaggaccgct cgcgtcaggt gatttcggca tcgccccagc
     8641 gtagttgacc tgctggttag cccctaatca atcggtcctc tacgattctg caggtgcgca
     8701 gacttcgcag gcccgttcgc ctgatcaccg tgttcaccgg gctggccgcg gccacctcgg
     8761 tgctggcggc tcccgctcag gccgaacccg acaccgacgc gttcctgagc gcggtgacga
     8821 gcgcgggcct cggcggcacc accgatccgt cgaacctggt cacggtcggc caggccgtct
     8881 gcccgatgtt gtccgacccg ggccagaacg ccgccgacgt cgccgcgaag gtcgccgaca
     8941 cgacgggaat gtcgctcggc ggagcgaaca tgttcaccgg gcttgcgatc tcgttcctct
     9001 gcccgcgggt gatggaatcg gtcgccggcg gccagtcgcc gattccgctg ggcctcctcg
     9061 gcttttagcc ggccacactt tccaacctcg ggcgtttcgg cgcacggccg tccccggtgg
     9121 agatgccgag ggcgtggtgc cacagccatg gggcgagcat gttcctggtc cacagaacct
     9181 gtgaatgggc gcgtgaccgc attgtcgacc gtgcggcggc cgcgccggcg tatgcccagt
     9241 catggttgct tccaggtaga ttgagtgctt cggccgccgc ggcggcgaac agcatgtggc
     9301 ccttggtcga accgtgcacc cggtcgtcac tccacacgtc gggttcggtc atcgacgggg
     9361 cgccgtagag gtcgacgagc ttgaacccgt agcggtcggc cgcagcgacg atcgcccggt
     9421 tcgtgcgcga catacgcgcc gtcaagatct tgccgatcgg cacgatgcga gcgatgtcgg
     9481 gaaacgtggt ggtcacgatg gtcgcgccgg attgcgcgag ccgttcgtac agtagatcga
     9541 gatcggccag cgcccggtcg aacagcagcc ccggcctggt gatgtcgttc attccgacgc
     9601 atacgctgac cagatcgggt cgcatcgtca aagccagggg cagttgatcg atgagcacat
     9661 cgcggatccg gcggccgcgg atcgccaggt tggcgtactg caagccggga ctgagctcgt
     9721 ccagcatcgc cgccagccgg tcggcgaagc cgacgattcc atcggaatcg tcgccgtccc
     9781 agagcccttc ggtctggctg tcgcccagcg ccacgtacct ggtgtaggtc ccgtgcacca
     9841 tgaccgtgac tctatcggtt atccgcggcc gaaccgggtc aggttgagcg gacggccgac
     9901 cgcgctgagc tgagtgatga cgtcctcgat gcgcgtgcgc gggacgtcga tccgccactc
     9961 ctgaagcacg ccggacagac acagcttgtc gggctcgaac ctgatgtcgg tcaaccgaag
    10021 tccgtgtgga agctctggca gctgcacggc atgagccggc gtgcgcttgg gcaggcccag
    10081 acgtcggcgc cgcaccacca ggccgcgcgg cttgagccac aaggtggatc catccagaac
    10141 cgcgtccacc tccagatgac ccagcgacgg gcggcgggcg agccgcaaac gtgcgatgcc
    10201 gtcagggccg atctcgcctg ccagccgcgg tgccaccaag cggaacaggt cgtcgagtgc
    10261 gtctgcgggt agttcgacgg tcaggtcgac aggtgccgcc accagcaccg gcggcgccga
    10321 cggccgcagg tgcacgttgt gcaggaccgc ggtggcgcga tccaatctgt ttctgtccca
    10381 acggatatcg cgtgccgcaa gccaaacatc gctcaattga ccggcagcga gaccacggac
    10441 gtcgagacgc gagtcgaatt cggtcacggt cagcgtgatg tcaccctcgt cgaggcggac
    10501 cgacaactcg cgcccgacca ccagccggcg aagcgtcatg aaaagcgtcc ggtacgccgc
    10561 ggcggcaccg gtgctgacgg gtgcgacggc cgccgtcgac cacagggagc cgagcatgtc
    10621 gagcggccgg aagtgatccc atcggcgcaa cggtcgcgaa cgcgtcatga cacccagcat
    10681 cctcgcactg cccgtcgggc cacggcgagt cgcctcaggg ggcgaagctg ttcaacagcg
    10741 cgcgcatcga gttggccacc gcgtgtcgcg tcttcaccgg atcgggtgag ttggcgatca
    10801 acatcgcccc gcgggtcagg gcgccaagca gcaaccgggt tgcagtctcc gggtcgtcga
    10861 tgtcgaccgc gcccgccttg gccgcctcct tcagcgatcc cacgatcacc gcgtaggcgt
    10921 actgctcgtc gagctcggtg tagcgcgcca gtcccagcac agcgggcccg tctagcacga
    10981 cgatccgttg cacatcgggc tgcaacactc cgtcgaggaa cgcgtcgaag cccagcgcca
    11041 gctcgtcgag cccgtcctcg gcgcccatcg ccgcctctgt ggccgacgtc atcagttcca
    11101 tgtgcgcttg cacgaacacc gcttcgaaaa gtgctgtctt ggtagggaag tggtggtaga
    11161 gggcaccggt tgtcacgcgc gcctcacggg ccagatcctc caccggcgtc ccccggaaac
    11221 cgttctcgcc gaacagcctg cggccggccg ccaccagtgc tgcacgcgtc tgtgccacct
    11281 gctcggcgcg cagcgacgac ggctctgagg cgggcatggc tctcacattc ggtggtggac
    11341 gggtcgagcc taccctattg acatagcaac gatatgtgaa atagttcctc tatgactatt
    11401 gctcccatgc gtaccaccgg accggccgaa gacacccgtc gcgcccacgg ggcgaccaga
    11461 gacgccttag gcgccgccgt gcgcaggacc gcggagcttt tccagcgggt cgacgatgcc
    11521 gacgcaccgg tgccgggact ggaatggacc gttgcggaga cggccgcgca catggtcggc
    11581 gaactgcgtg attacggcca ggcgctgacc cgacacacga acggctatat gacgcacgcc
    11641 aatcgggccc aggaatcgcc gtcacggatg agcgcggtcg tcaacgcccg tcaactcacc
    11701 gagatacccg aaagggatat gaaccggctc gccggactgc tcgaggacgc cgccctggcc
    11761 tatttgactg ccgcggctgc tgctgacgag accgcggcga tcccggccgc gaacgggcta
    11821 gtactcaatc catcgacgat gaccagcctg ctgcttggcg aacaggtcat tcacggcctc
    11881 gacatcgcca gggcggtcgg cgcccgttgg gacatccgag gccacgacgc cctgctgatc
    11941 atccccggcg cgctgaccgt cgcgccgcac tacctgcgac cgtccgctgc cgctgcgcgg
    12001 gtgagtttcg agttgcgtat tcggggcgcc aatacatacc ggatggcggt ggaccgcggg
    12061 accgccgttg tcaccggcgc ggcggacagg tccgactgcg tgatcaccgc cgaccccgtc
    12121 gcgttcctgc ttctcggctt cggacgcgtc tcgcagtggt cgccgattct gcggggcaag
    12181 cttcgggcag gcggcaggaa gccgtggtcg gcaatgaaat tcgccacgct gttatccagc
    12241 ccatagtggg tagcccccct accgtggact caaagacatc cgtcgtcatc gttggcagtg
    12301 gcttcaccgg tttcgagtgc gcacgccgtc tctctcgaca attgcgcaag cacgacgccg
    12361 ctgtggacat ctcgatcatc tccccggtcg actacatgct ttacacgccg ctgctgcccg
    12421 atgtggcggg tggcgtggtc gacgcccggt tcgtcaccgt cccgctggcg aacacgctgc
    12481 acggcgtgcg cgcggtgcgc gggcgggtcg acagcgtcga cctcgacggc cataccgtca
    12541 cctacaccga ccccgaacaa cgatcgcgca atatgtcgtg ggaccggttg gtggtgacgc
    12601 cgggttcggt cacccgattg ttcgacatcc cgggtctcgc cgagcacgct aggggtttga
    12661 agtcgactgc tgaggcggtc tatctgcgcg atcatctgct cgaacaactc gagttggcca
    12721 acatcgacga cgatgaggcc cgcattcagg cgaggcgcac ggtcgtcgtc gtcggggcct
    12781 cgtattcggg caccgagctc atcgtgcagc tgcaggcgct ggccgacacg gcagcaaagc
    12841 agaccggctt cgacccggcg ggcatgaagt tcgtgcttct cgacatggcc gagcaggtga
    12901 tgcccgaggt gggagagaag ctcggcaagg gcgttcagcg ggtgctgcac caacgcggca
    12961 tcgacgtgcg actcggcctc acgttgaaag aagtccacgc agaccatgtt gtgctcagcg
    13021 acgagacgcg aatcgacacc cataccgtcg catgggtgac cggggtcacc ggcgccccgt
    13081 tgatcgagac cctgggactg ccgaccgagc gtggccggct gaaggtgcag gccaacctgc
    13141 aggtgcccgg gcatccagat gtgttcgccg ccggtgacgc cgccgccgtt ccggacctga
    13201 cccagcaagg cgagatcacg ccaccgaccg cacagcacgc gatccggcag ggaaaggtgc
    13261 tggggcgcaa cgtcgccgcc agtctcggct acggcgaggc gaaagactac cggcatcgca
    13321 acatgggtct ggtcgtcgac ctcggcccgc attatgcggt cgcgaacccg ctgaacatcc
    13381 agctgtccgg ctatccagcg aagttcgtca ccaggatgta tcacctgtat gccatgccgc
    13441 gcttcgtgaa ccgatgggca gtatcgctgt cgtatctgac cgacgtgttc ttcgaccgct
    13501 cgatcgtctc ggtcggcctg tccagtcagg acgacgccag cttctccacc agcgagggca
    13561 ttccgctgcc gaaattcgac tgacgtgtga aaagcggcgg acggggaact cccgtcccat
    13621 ggctacctat cgcgtgctga acccgaaggg tgacgtcgtc gacaccaagg acatcgagag
    13681 cgcgcaggat gcgcacgcct ggttcgtcga cgcgaaagcg gacaactccg aactcggctg
    13741 gcgcatggaa gtcgaagccg acgacggcca ttgggccttc ttcgatgaca ccgaaggcga
    13801 ccggaagtag tcagtcttcg ccggtgacgt accacttgcc tatcggcggc gcgtggctgc
    13861 tgcggacccg caggtatcgc ggttcttcga cggagaacgt cggatagtca cccagacaac
    13921 aactctcacc ggtctttatc ttggtgatcg tctgcgtgtc gcggtcgaac gtcacggtgc
    13981 cgtcgccgtc tagatccagc cgccatcggg gctcgaagtc acccatcagg cgaccgtcag
    14041 ggtgacgttg agcagggaaa cggcgacatc gctgggcagg atgtcgacga acgtgggctg
    14101 gtcgcgcaac gcttctcctg tgccgatgat gttctgagga tagttgccag cacacggaaa
    14161 gctgttgcag cgcagccaca tcccaggcgg gctctgatac ggatacatgc tcggagactg
    14221 gtcgacgcga tacctccccg gcgggatgct ggcagtcgac cagccctgcc gcggctccgc
    14281 gccgacggag aagacgccgt tgctgccata gctcggtgga tcggcgttcg ccggcgccgc
    14341 cacgacgagg cctgccatca gtgcgcctgc cgccaacccg ccggctgcgc ggttcatcgt
    14401 ctcaccacac ccgcacgtaa tcgatgagca tttccgggga ctggaagggg ccgaaggccg
    14461 gatctccgcc gccgggaccg ccgaccgcaa ggttgaacac cgtgtagagc agatagtcgg
    14521 gcgcgttgaa cggccaaacc ccgtcgatgg gcaccgaggg aaccgtgaag tacggcgctc
    14581 cgttgacgtc gtcgcgccag aagcggaagc cggcgtcgtc ccaccgcacc cggtaggtgt
    14641 gccagtcccc gtccaccggc cacctgttct ccttccaggt cttgccgtcg gaccgggcgt
    14701 gcactgccgt ccccggcgcc cactcgccgt ttccgtacca ctcgaggatg tcgacctctc
    14761 catcgggcag ggggcccctg ccgtcgggcg tgggctcccg gttcaccagc cagtaggccg
    14821 gccagcaacc cggaaccagg cagttcagct tcatgcgggc ttcccaggta tgtccgatgc
    14881 cgatcttcat cttgctctcc acccggccgc tgaaatacgt gtcgccctcc cgggtggcgc
    14941 gcagcaccag gttgccgttg ccgtcgaggg acacgttgcg gcgatcgtcg cggaaaaggc
    15001 ccaggatcgg cgggttgacc ggctcattcc agcccaccac cgtccacctc ccgaagtccg
    15061 gcggtgcgcc tgccggcccg tcgaagtcgt cgaccaggat gtagtcgacg ggggcggggt
    15121 tggcattcgg cggcggcgca cccggattct ccggctgacc tggcgctaag ggatcggcgg
    15181 cggcctgcgg agcggtgatc gcggcggcca gcgcggcggc cccggtcagc atcatcaaat
    15241 tgcggcgatt catctctgtc acaccaaaac cttagcgtcg gttaatcgaa gatttaagta
    15301 ggctggggtc taatgccgag gctcagtgcg ggactactgc tgtaccgggt cgtcgacggt
    15361 tccgttgaag tgctgatcgg gcatcctggc ggcccgttct gggcaaagaa ggacgacggg
    15421 gcgtggtcga ttcccaaggg ggagtacacc gacggcgagg acccttgggc ggccgcccgc
    15481 cgggagttcg aggaggaact cggcaagccg ccacctgacg gtccgcgcat cgacttccca
    15541 ccgctgaagc agccgagcgg caagatcatc accgccttcg cggtgcgtgg ggacctcgac
    15601 ctcgagggaa ctgtgagcaa caccttcacc ctggagtggc cgaaaggatc gggcaacgtc
    15661 agggaatatc cggagatcga ccgggcggac tggttctgcg tgcctgcggc gcggtcgaaa
    15721 ctgctgaagg gtcagcgcct acttcttgat cggctggtgg atgtgctcgc aaacgccgaa
    15781 taggccgcat ctcaatggca ttgatggtca aggcccggcc gtgtatgcgc cagccggacc
    15841 gcgtgcgcag gtactcgtcg tcgtagcgca ggtgccagac gaggtccgtc gcctcggcac
    15901 tgctgacgct gaaatgatgt gcgatgcaag tgatccggcc cagcgcgtag tcggcgtccg
    15961 cgccgggggc atagacctca ccgacgatcg cgtgctcggt gcgggtgacg cccgacagcg
    16021 ccgccatggc cgtgcgcaca ccgtcgcggc cttgatggtg gcggacgggc tcaagcgacc
    16081 gaggcggatc cggcagtcgt agttcggctg tgtcagtgaa caattcgacg acatccccga
    16141 accggcggtc atcgacggca gcggcgtaga ggtgaaccag atcagcgagt gccaaccggt
    16201 cggcgatggc gagggacatg tcgctcagct gccggcttgg ttgccggccc ggatgtcctc
    16261 gcggcactgg cgccgcgtct tgccggtctc ctgcatgcag acccgcaccg gggcctcctc
    16321 tgcgacgggc agcggttcgt cggtcgcctg agtcgtcacg gtgatggtcg gcgcaggcac
    16381 cacaccctgg gtgaccgacc agatggatgc gccggtgccc tccagcgttg agcagtacac
    16441 cgtgtttccg ttggcggtgg tcgcggtgtc gccgaccggc gagcagtccg cgccgacgac
    16501 cgcggccgcc ggtgcggccg acgccgtggt ggtggtcgtg ggcgacggcg tcgactcgga
    16561 cggcggcggc ggtagtgccg gtggcgcttc ggcccgaggg gaggtcgcgg gcggcctcgg
    16621 cgacggggtg tagtcgtcgt tcagcagttt ctcgatctca cggaactgga tcagcacgag
    16681 ggcg
//
LOCUS       DASYLX010000248         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_559058,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000248 DASYLX010000000
VERSION     DASYLX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559058"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGP27_06155"
     CDS             complement(<1..255)
                     /locus_tag="VGP27_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV7850931.1"
                     /translation="MSDGVKLVDRVSAINWNRLQDEKDAEVWDRLTGNFWLPEKVPVS
                     NDIPSWETLNAHEKQLTMRVFTGLTLLDTIQGTVGAVSLIP"
     gene            complement(392..1912)
                     /locus_tag="VGP27_06160"
     CDS             complement(392..1912)
                     /locus_tag="VGP27_06160"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850932.1"
                     /translation="MTVAEQSAEGNTQTAIYTRALIIGTGFSGLGMGIALKKQGVDFL
                     ILEKADDVGGTWRDNTYPGAACDVPSHLYSFSFALKSDWPHVFSYQPDILTYLKGVAD
                     KYGLKRHIRFGQHVDRAHWDDSEFRWHVFTKSGEEYVAQFLISGAGALHIPSLPDVAG
                     LGDFHGPVFHTAQWDHTVDLTGKRVAVIGTGASAIQVVPELVKIASQVQLYQRTPAWI
                     KPRINFAFPDSMKRAFKFVPGLRAALRTATYWALDARAFAFTKHPSLLRFVEPVYELN
                     MRRYVADPQLRRKLTPSYRAGCKRILISDNFYQAVADPKTDVITDGIARVIRDGIVTV
                     DGTERKVDVIVCATGFHVTDWYNYLDIKGAHGEDLGDRWNREGVMAHRGITVADVPNL
                     FLLVGPNTGLAHNSMVFMIESQIGYVADAIGAADRLGAQALAPTRHAQDQFNEELQRD
                     LSGTVWNTGGCQSWYMDDHGVNRSLWSGFTWEYWLATRKFKMSEYSFSGVGQPVAV"
     gene            complement(2064..>2940)
                     /locus_tag="VGP27_06165"
     CDS             complement(2064..>2940)
                     /locus_tag="VGP27_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005260907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850933.1"
                     /translation="GAGIASPPTAATRDDLDRTGALRQWWLLTRRNADVLLRNRLTLA
                     ILLGSPVLVTAMMATLFTRGAFDFSTAAGVGPAQIVFWIAFDGFFFGLTYGLLQIVGE
                     MAVFRRERLAGLHVGAYVASKVTTLLPVLAAVSAVLLLVLRALGRLPAVGWHVYALLF
                     VTIVIEAMSALALGLLASAAVSNAAQAALALPMLCFPQVLFGGAIVPVDAMATPGRLI
                     SLGLSNRHAFEALGRDLNLDRYTATLPAMSVYRDTFDGGTGQSLITLAVVTIVLLLAT
                     VWVLDRRSAPGAFRL"
BASE COUNT          575 a          926 c          940 g          499 t
ORIGIN      
        1 ggggatgagg ctgaccgcgc ccaccgtgcc ctggatggtg tccagcagcg tcaggccggt
       61 gaagacccgc atggtcagct gcttctcgtg agcgttcagc gtttcccagg acgggatgtc
      121 gttggacacc ggcaccttct cgggcaacca gaagttgccg gtcagccggt cccacacctc
      181 ggcgtccttc tcgtcctgca agcggttcca gttgatggcc gagacacggt cgaccagctt
      241 cacgccatca ctcacgagaa cctcacttca cctggtcttt taggtctgag caacagagtc
      301 gctccagcgc cctcaacact acaactgggg tccgactttg caccgcaaca caacatcatg
      361 tgttcagcgt gtcgggcgcc cgagtgtgca actagacagc cacaggctgc cccacaccgg
      421 agaagctgta ctcagacatc ttgaacttgc gggtcgccag ccagtactcc caggtgaagc
      481 ccgaccacaa tgaccggttg acgccgtggt cgtccatgta ccaactctgg caaccaccgg
      541 tgttccacac cgttccggac agatcgcgct gcagctcctc gttgaattgg tcctgcgcat
      601 ggcgagtcgg cgccagcgcc tgcgcgccca gtcgatcagc ggcaccgatc gcgtcggcga
      661 cataaccgat ctgcgactcg atcatgaaca ccatcgagtt gtgtgccagg ccggtgttgg
      721 gaccgaccag aaggaacagg ttgggcacgt cggcgacggt gatgccgcgg tgcgccatca
      781 ctccttcgcg attccagcga tcgccgaggt cttcaccgtg ggcacccttg atgtcgaggt
      841 agttgtacca gtcggtgaca tggaaaccgg tcgcgcagac gatgacgtcg accttgcgtt
      901 cggtgccgtc gacggtgacg ataccgtccc ggattactcg tgctatccca tcggtgatta
      961 cgtcggtctt cgggtcggcg acggcctgat agaagttgtc cgagataagg attcgtttgc
     1021 agccggcgcg gtaggacggc gtcagcttgc gtcgcagctg agggtcggcg acgtatcgcc
     1081 gcatgttgag ctcgtaaacc ggctcgacaa accttagcag cgacgggtgc ttcgtaaacg
     1141 cgaaggccct ggcgtccaac gcccagtagg tggcggtccg cagcgccgcg cgaagcccgg
     1201 gcacgaactt gaacgcccgc ttcattgagt ccgggaacgc gaagttgatg cgcggcttga
     1261 tccacgccgg agtgcgttga tacagctgaa cctgactggc gatcttgacc aactcgggaa
     1321 ccacctggat cgcgctggca cccgttccga tcaccgcaac gcgcttaccg gtgaggtcga
     1381 cggtgtggtc ccactgtgcg gtgtgaaaaa cagggccgtg gaagtcgccg agtccggcta
     1441 catccggcaa cgatggaata tgcagcgcgc cggcacccga gatgaggaac tgggcgacat
     1501 actcctcgcc gctcttggtg aagacgtgcc agcggaactc ggagtcatcc cagtgcgcac
     1561 ggtcgacatg ctggccgaac cgaatgtggc gcttcaaccc gtacttgtcg gcgacgccct
     1621 tgaggtacgt gaggatgtcg ggctggtagg agaagacatg cggccaatcg gatttgagcg
     1681 caaaggagaa cgaatacaga tgcgacggca catcgcacgc cgcgccggga taggtgttgt
     1741 cccgccaggt gccgccgaca tcgtctgcct tctccagaat caggaagtcc acgccctgct
     1801 tcttcaatgc gatacccatg cccagtccgg agaagccggt cccgatgatc aacgcccggg
     1861 tgtagatcgc cgtctgagtg ttgccttcgg ccgactgctc tgcgacggtc atgcttcctc
     1921 cctgccttcg gtgtgggccc agcctgggaa cggcggtacc gggtattcgc tcacgccagt
     1981 gtaagcggca ggaccctgac tgggtcgccg cacgccaacc cgcgagatgc atcagtcatc
     2041 tcgcgggcgg gcatgctagg ccgtcatagc cggaatgcgc ctggcgcgga ccgccggtcg
     2101 agtacccaga cggtcgcaag cagcaggacg atggtgacga cggcgagcgt tatgaggctc
     2161 tgtcccgtcc ctccgtcgaa ggtgtcgcgg tagacggaca tggcaggcag agtggcggtg
     2221 taccggtcga gattcagatc gcgacccaat gcttcgaacg cgtggcggtt ggacagaccg
     2281 aggctgatca ggcgtccagg ggtggccatc gcatcgacgg gaacgatggc gccgccgaac
     2341 agaacctgtg ggaagcacag catcggcagg gcgagggccg cctgcgcggc gttggagacc
     2401 gccgccgacg cgagcagtcc gagcgccagc gccgacatcg cctcgatgac aatcgtcacg
     2461 aacagcaagg catagacatg ccagccgacg gcgggcagcc tgccgagcgc gcgcagcacc
     2521 aagagcagca ccgcactgac cgcggctaat acgggaagca gcgttgtcac tttggaagcc
     2581 acgtaggcgc cgacgtgcag gcccgccagg cgctctctcc gaaaaaccgc catctcgccg
     2641 acgatctgga gcaggccgta cgtcaggccg aagaagaagc catcgaaggc gatccagaac
     2701 acgatctgcg ccgggccgac gcctgctgcg gtactgaagt cgaacgcgcc gcgcgtgaac
     2761 agcgtcgcca tcatcgcagt gacaagcacc ggggagccga gcaggatcgc cagggtcagc
     2821 cggttgcgca ggagcacatc cgcattgcgt ctggtcaaca gccaccactg ccgaagtgca
     2881 cccgtgcgat cgaggtcgtc ccgggtggcc gcagtgggcg gcgaagcaat gcctgcacct
//
LOCUS       DASYLX010000249         4046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2204209,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000249 DASYLX010000000
VERSION     DASYLX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204209"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VGP27_06170"
     CDS             complement(<1..723)
                     /locus_tag="VGP27_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7850934.1"
                     /translation="MTTDTPAGQADEVDQIPRRQLPFWVALALSVALVGPTLAMSGNG
                     QGLIGTVGKSIPLVFLIGLVGVSLVGYSFVRLTRHLNHAGSSYGLVGGTIGPRAGFFA
                     GFAMLGAYWAFSIGTLALTAAFTNAFIAALQPDSDNPYQAPWVVIVVIAAVVSFLLSG
                     RDIRLLAKILLAIEGIGILAMVILVIVIFAKGGAPSTGIDFSVFSFSGGGVTPSAVLA
                     GVVAAFLSWAGFEACASMGEETD"
     gene            complement(753..2069)
                     /locus_tag="VGP27_06175"
     CDS             complement(753..2069)
                     /locus_tag="VGP27_06175"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV7850935.1"
                     /translation="MPLPTPEELRADGVALIAGSITDLAGVTRAKYVPLSRLAAFQRS
                     GMGVSPSWSVFCVDSGIAFTPNIGVDGDLRIRIDPADLRLVEDGVAWAPGDLTDQQGD
                     PAALCTRSLLKGIESELTAHGLTALVGAELECTMLAPDAGHASADPWSPYGLRTSLER
                     SAFLVDLAAAAERADLPVEQIHTEYGHDQLEVSLAPTTPVAAADAVAAARIVIGRAAA
                     RHGLRISFSPVPFEGEAGNGAHLHLSLADEDGPLFSGGQGPYGMRPAGESAIAGVLQT
                     LPDLLGVYAGSVLSSARLKPGNWAGAAQCWGLENREAAARFVAATPGTPHGANIELKL
                     IDPSANPYLATVAFVGSALRGIDQRLDLPAEVPKDPSKAAQPPPMLQTDQQTVIKALE
                     ASPVAAELLTPVVVEGLVAVRRYEITTYGDRPPAETAQALRLAWSC"
     gene            2196..3002
                     /locus_tag="VGP27_06180"
     CDS             2196..3002
                     /locus_tag="VGP27_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEV7850936.1"
                     /translation="MCRLLGVVSATPVSVSQAVGEHVLKDFVALTRIHGDGWGVARVR
                     LPGHHPKTEVSAGSALDDPDFAAATHDKRSAASLVHLRWATTGIAVRPENSHPFLADR
                     IAMAHNGSVKPIAQLEKMVAPATAATLRGATDSERYFALIRQHRASSPNLAEAVRRAV
                     SQLREVFPDASLNALILGEDQLVAVHAHARSRLPDEDIAEITAADLPAEHLEDYFGMR
                     WARVDDDTVVIASTGFGDLAWRPLESESVTTISMCDLSMTTLPLMTNSTP"
     gene            2951..3853
                     /locus_tag="VGP27_06185"
     CDS             2951..3853
                     /locus_tag="VGP27_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV7850937.1"
                     /translation="MRPVDDDPAAHDELDTVTRETVAVRAGSALPALSKAERRVGRAL
                     LADYPSAGLASAARLAERAEVSPPTVLRFAQSLGYDGFTDLQVALRAELTAQSNGPIT
                     RLPDAQPAGGLLDRLLQQARAQNERAVETLAALPNSAIEAAVALLSDTGRAVYLHGGR
                     FSHLLAVHLAAHLEQLRPGVRLLADPDGSDMGAMLELSRADVVVLFDYHRYQRSAAEL
                     ALRVHRAGATVLLVTDDLACPVAPDAEVVLAASSTVGTTYQSMAAGFLLTELLIPLVM
                     DALGEPARTRMALWEEQRRTELLP"
     gene            3871..>4046
                     /locus_tag="VGP27_06190"
     CDS             3871..>4046
                     /locus_tag="VGP27_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7850938.1"
                     /translation="MAGGTKRLPRAVREQQMLDAAVQMFSINGYHETSMDAIAAQAEI
                     SKPMLYLYYGSKEEL"
BASE COUNT          672 a         1356 c         1383 g          635 t
ORIGIN      
        1 gtcggtctcc tcacccatcg atgcgcacgc ctcgaaaccc gcccaggaca ggaacgccgc
       61 gacgacgcct gcgagcaccg ccgaaggtgt gacgccgccc ccggagaagg agaacaccga
      121 gaagtcgata cccgtcgacg gcgcaccgcc tttcgcgaag atcacgatga cgaggatgac
      181 catggcaagg atcccgatgc cctcgatcgc gagcaggatc ttggcgagca gcctgatgtc
      241 gcggccggac agaaggaacg agacgacggc ggcgatgacg acgatcacca cccacggcgc
      301 ctggtacggg ttgtcgctgt cgggctgcag cgcggcgatg aacgcgttgg tgaacgccgc
      361 ggtcaacgcg agcgtgccga tggagaacgc ccagtaggcg ccgagcatcg cgaagcctgc
      421 gaagaacccg gcccttggtc cgatggtgcc gcccaccaga ccgtaggacg acccggcgtg
      481 gttgaggtgc cgggtcagcc gcacgaagct gtaaccgacc agcgagacgc cgaccaggcc
      541 gatcaggaac accagcggga tcgacttccc cacggtcccg atcaggccct ggccgttgcc
      601 cgacatcgcc agtgtgggcc cgacgagggc cacagacagc gcgagcgcga cccagaacgg
      661 cagctgccgc cgggggatct ggtcgacctc gtccgcctgt cctgccggtg tgtccgtggt
      721 catcgatgtc tccttctgtc gagaaaaacg ggtcaacagc tccaggccag tcgcagtgcc
      781 tgggcggttt cggccggtgg ccggtcgccg taggtggtga tctcgtagcg ccggaccgcg
      841 accaatcctt cgacgaccac cggcgtcagc aattcggcgg cgacgggcga cgcctcgagg
      901 gccttgatca cggtctgctg gtcggtctgc agcatcggcg gcggctgcgc ggccttcgat
      961 gggtccttcg gcacctcggc gggcaggtcc agccgctggt cgatgccacg cagagcgctg
     1021 ccgacgaacg ccaccgtggc cagatagggg ttagcgctgg ggtcgatgag cttgagctca
     1081 atgttggcgc cgtgcggcgt gcccggcgtt gccgccacga atcgcgctgc cgcttcccgg
     1141 ttctccaggc cccagcactg cgccgcgccc gcccagttgc ccggcttcaa ccgggccgac
     1201 gacagcaccg aacccgcgta gacgccgagc aggtcgggca gtgtctgcaa cactcccgcg
     1261 atcgcggact cacccgcggg ccgcatgccg tacgggcctt ggccaccgga gaacagcggc
     1321 ccgtcttcat cggccagcga caggtgcaga tgggcgccgt tgccggcttc accctcgaac
     1381 ggcacgggcg agaacgagat gcgcaagcca tgccgtgcgg cggcacgccc gatgacgatg
     1441 cgtgccgccg cgacggcgtc ggcggcggcg acaggcgtag tcggtgccag cgatacctcg
     1501 agctggtcgt ggccgtactc ggtatggatc tgctcgacgg gcaggtcggc ccgctcggct
     1561 gcggccgcca ggtcgacgag gaacgccgag cgctccaacg acgtgcgcag accatagggc
     1621 gaccacggat cagcagacgc atggcctgca tcgggggcca gcatcgtgca ctccagctcg
     1681 gcgccgacca aggcggtcaa gccgtgtgcg gtcaattcgg attcgatgcc cttgagcagc
     1741 gagcgggtgc acagcgccgc agggtcgccc tgctggtcgg tgagatcgcc cggtgcccac
     1801 gccaccccgt cctcgacgag ccggagatcg gcgggatcga tacggatccg gaggtccccg
     1861 tccacgccga tgttcggggt gaacgcgata ccgctgtcga cacagaagac gctccacgac
     1921 ggggaaacac ccatgccgga gcgttggaat gcggccagtc ggctcaacgg cacgtacttc
     1981 gcgcgggtca cgccggccag gtcggtgatc gaaccggcta tcagggcgac gccgtcggcg
     2041 cgcagttcct ccggggtggg cagcggcatt gaacctcctc gcacgtcacg agtctgcgtg
     2101 ccatttatgt cataaatgtt tcataatctc taagatctcc gctagaggaa tcattcatta
     2161 aattttcgtg tcttgtccgt cgaaggggtg ttgccatgtg ccggctgctg ggagtcgtct
     2221 ctgccacccc ggtgtcggtg tcgcaggcag tcggtgagca cgtcctgaag gacttcgtcg
     2281 ccctgacgag aatccacggc gacggctggg gcgtggcgcg ggtccgactg cccgggcatc
     2341 acccgaagac cgaggtgtcg gcaggcagtg cgctcgatga tccggacttc gccgcggcga
     2401 cgcacgacaa gcggtcggcg gccagcctgg tccacctgcg ctgggcgacg accgggatcg
     2461 ccgtgcggcc ggagaactcg catccgtttc tggctgaccg aatcgccatg gcgcacaacg
     2521 gttccgtcaa gccgatcgcc cagctcgaga agatggtcgc gccggcgacc gcggcgacgc
     2581 ttcgcggcgc caccgacagc gagcgctatt tcgctctgat ccgccagcac cgcgcctcgt
     2641 caccgaatct ggccgaggcg gtccgcagag cggtctcgca gctgcgcgaa gtctttcccg
     2701 acgctagcct caacgccttg atcctcggtg aggatcaact ggtcgccgtg catgcccacg
     2761 cccgcagccg gttgcccgac gaggacatcg ccgagatcac ggcggcagat ctgcccgcgg
     2821 agcacctcga ggattacttc gggatgcgct gggcacgtgt cgacgacgat acggtcgtga
     2881 tcgcgtcgac cggtttcggc gacctggcct ggcgaccgct cgagtcggaa agcgtcacga
     2941 cgatctcgat gtgcgacctg tcgatgacga ccctgccgct catgacgaac tcgacaccgt
     3001 gacccgcgaa acagtcgccg tgcgggcggg ttcggcatta ccggcactca gcaaggcgga
     3061 gcgtcgggtc ggtcgggcgc tgctcgcgga ctatccgagc gcaggtctgg ccagcgcagc
     3121 ccggctggcc gaacgcgccg aggtcagtcc gcccaccgtg ctgcggttcg cgcagtcgct
     3181 cggctacgac gggttcaccg accttcaggt cgcgttgcgg gcggagctga cggcccagtc
     3241 gaacggcccg atcacccggt tgcccgacgc gcagcccgca ggcggtcttc tggaccggtt
     3301 gctgcagcag gccagggccc agaacgagcg ggcggtggaa acgcttgccg cgctgccgaa
     3361 ttcggcgata gaggccgcgg tcgcgctgct gtccgatacc ggccgggcgg tctatctgca
     3421 cggcgggcgc ttctcgcacc tgcttgccgt gcacctggcc gcccacctcg aacagctgcg
     3481 gcccggtgtg cgcttgctcg ccgatccgga cggaagcgac atgggtgcga tgctcgaact
     3541 gtcgcgcgcc gacgtcgtcg tgttgttcga ctatcaccgc taccagcgca gcgccgccga
     3601 gctcgcgctg cgtgtgcatc gggcgggggc gacagtgctc cttgtcaccg atgacctggc
     3661 gtgtccggtc gcgcccgacg ccgaggtggt gctcgccgcc tccagcaccg tgggaacgac
     3721 ataccagagc atggctgccg gatttctgct gaccgagctg ttgattccgc tggtgatgga
     3781 cgcactcggc gaaccggcgc gcacgcggat ggcgctgtgg gaggagcagc gccgaaccga
     3841 actgctgccc tgagccggta ttctggaccg atggcaggtg gtaccaaacg gcttccgcgt
     3901 gccgtccggg agcagcagat gctggacgct gcggtgcaga tgttctcgat caacggttat
     3961 cacgagacgt cgatggatgc gattgccgcg caggcggaga tctccaagcc gatgctctac
     4021 ctctactacg gttccaagga ggaact
//
LOCUS       DASYLX010000250         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_10505, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000250 DASYLX010000000
VERSION     DASYLX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10505"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGP27_06195"
     CDS             <1..576
                     /locus_tag="VGP27_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7850939.1"
                     /translation="GPAIVSGELPFPDDGRDPWRLDGTWTKPVVAAAHGWCMTLGIEL
                     LLAADIRVAAADTRFAQMEVRRGIYPFGGATIRLPQQTGWGNAMRWLLTGDEFDAQEA
                     LRIGLLQEVAANAAEAQSAARSIAHTIAERAAPLGVRAVLASAHLARTQGDDAAIERL
                     RPEMTRLFATADATEGVQSFIERREARFQGC"
     gene            618..2453
                     /locus_tag="VGP27_06200"
     CDS             618..2453
                     /locus_tag="VGP27_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7850940.1"
                     /translation="MGHYKSNVRDLEFNLYEVLELEKALATAEFGDLDGESVRQMLQE
                     ASKLAEGPLAEAFAETDRHPPTFDPETHVVTIPEPFKKSFRAWQQGEWFRIGLHEDIG
                     GVPAPAMVAWAVNEFPLGAQPAAFMYLAGPILANILYHIGNEEQRKLASIAMQRYWAA
                     TMVLTEPDAGSDVGAGRTKAVQQADGSWHIDGVKRFITNGDTDDLFENELHMVLARPE
                     GAGPGTKGLSLFVVPKFVPNLETGEPGERNGVFVTGLEHKMGLKASATCELTFGQHGV
                     PAVGWLVGDSHNGIAQMFRIIEYARMMVGTKAIATLSTGYLNALEYAKTRIQGADMTE
                     MTDKMAPRVTIIHHPDVRRALMTQKAYAEGLRALYLFTAAHQDPAVAEIVSGADAALA
                     ERVNDLLLPIVKGVGSERAYQCLTESLQTFGGSGFLQDYPIEQYIRDAKIDSLYEGTT
                     AIQAQDFFFRKIARDQGVALAHIVAQMQKFLDRETARPELAEGRALLATALADAQAMT
                     ATLTGYLLGAQENARELYRLGLESVAYLLAVGDLLIGWLLLEHAEIALTALDGDTSDR
                     DRSFYRGKVAAAEFFARNVLPRLAADRKIVEGVDLTIMDLREEAF"
     gene            2453..2911
                     /locus_tag="VGP27_06205"
     CDS             2453..2911
                     /locus_tag="VGP27_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7850941.1"
                     /translation="MKVITSIDDAVATVGEELGVGEWQEVDQDRINAFADATGDHQWI
                     HIDAERAKTQSPYGQTIAHGYLTLSMIPALTKDNYRVENAKMAINYGSNKVRFIAAVP
                     SGGRIRARSELIDATKVDDNTVNLTVKNTIELDGSNKPAAVAETIVRVLF"
     gene            complement(3069..>3413)
                     /locus_tag="VGP27_06210"
     CDS             complement(3069..>3413)
                     /locus_tag="VGP27_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEV7850942.1"
                     /translation="FFWWDARDMVASYGEFAAEHRAQHLTAFNRWCGVVGNYSIVPTA
                     VALLSGRPRLGTAVIGLGTAILLAGHAAEGNMPQSVRDFAEHPIWATRADFTLANATV
                     LNALKPPTNAIS"
BASE COUNT          631 a         1092 c         1146 g          544 t
ORIGIN      
        1 ggcccggcca ttgtcagcgg cgagttgccc ttccccgatg acggccgcga cccgtggcga
       61 ttggacggga cttggaccaa gcccgtcgtc gccgcggcgc acggttggtg catgaccctg
      121 ggcatcgaat tgctgctcgc cgccgacatc cgggtggcgg ccgccgacac ccgattcgcc
      181 cagatggagg tgcgtcgcgg gatctatccc ttcggtggtg ccacgatccg gctgccgcag
      241 cagaccggat gggggaacgc gatgcgctgg ctgctcaccg gagacgagtt cgacgcccag
      301 gaggccctcc ggatcgggct gcttcaggag gtcgccgcca atgccgcgga ggcgcagtcg
      361 gccgccagaa gcattgcgca caccatcgct gagcgcgccg cccctctcgg tgtccgggcg
      421 gtgctggcgt cggcgcacct ggcccgcacc caaggcgacg atgccgccat cgagcggctg
      481 cgcccggaga tgacgcgact cttcgccacc gcggacgcca ccgagggggt gcagtccttc
      541 atcgagcgac gcgaagcgcg gttccagggc tgctgagaac cgttacgctc ggctcagtcc
      601 cttcagaagg gcttgccatg ggccattaca agagcaatgt gcgcgacctc gagttcaatc
      661 tttacgaggt gctcgaactc gagaaggcgt tggcgaccgc agagttcggc gacctcgacg
      721 gcgagtccgt gcgccagatg ctccaggagg cgtcgaagct cgccgaggga ccgctcgccg
      781 aggcgttcgc cgaaaccgac cggcacccgc cgacgtttga tcccgagacg catgtggtga
      841 cgatccccga gccgttcaag aagtcgttcc gcgcgtggca gcagggcgag tggttccgca
      901 tcggcctcca cgaggacatc ggcggcgtgc ccgcacccgc gatggtggcg tgggccgtca
      961 acgaatttcc gctcggcgct cagcccgctg cgttcatgta cctggccggg ccgatcctcg
     1021 cgaacatcct ctaccacatc ggcaacgagg agcagcggaa gctggcatcg attgcgatgc
     1081 aacgctattg ggccgccacc atggtgctga ccgagcccga cgcgggttcg gacgtcggcg
     1141 caggccgcac gaaggcggtt cagcaggccg acggcagctg gcacatcgac ggggtcaagc
     1201 gcttcatcac caacggtgac accgacgacc tgttcgagaa tgaactgcac atggtgctcg
     1261 cgcggcccga gggcgccgga cccggcacca aggggctgag cctgttcgtc gtcccgaagt
     1321 tcgtgccgaa tctcgagacc ggagaaccgg gtgagcgcaa cggcgtcttc gtcaccgggc
     1381 tcgagcacaa gatggggctg aaggcgtcgg cgacgtgcga gctgacgttc ggccagcacg
     1441 gcgtgcccgc ggtcggctgg ctggtcggtg actcgcacaa cggaatcgcc cagatgttca
     1501 ggatcatcga atacgcgcga atgatggtcg gcaccaaagc tattgcgacc ctgtcgacgg
     1561 gctacctgaa tgccctcgag tatgcgaaga cgcggataca gggcgccgac atgacggaga
     1621 tgaccgacaa gatggcgccg cgggtcacca tcatccacca ccccgatgtg cgccgggcac
     1681 tgatgacgca gaaggcctac gccgaagggc tgcgcgcgct ctatctgttc accgccgcgc
     1741 atcaggatcc cgcagtggcc gaaatcgtgt cgggagccga cgccgcgctc gccgagcggg
     1801 tcaacgatct gctgctgccg atcgtcaagg gtgtcggctc cgagcgggcc taccagtgcc
     1861 tgaccgagtc gctgcagacg ttcggcggat cgggcttcct gcaggactat ccgatcgagc
     1921 agtacatccg cgacgcgaag atcgactcgc tgtacgaggg caccaccgcg atccaggcgc
     1981 aggacttctt cttccgcaag atcgcgcgcg accagggtgt ggcacttgcg cacatcgtcg
     2041 cgcagatgca gaagttcctg gaccgcgaga ccgccaggcc ggagctggcc gagggccgcg
     2101 cgctgctggc cacggccctt gccgacgcgc aggcgatgac ggctacgttg acgggatatc
     2161 tgctgggtgc gcaggagaac gcccgcgagc tgtatcggct cggcctcgag tcggtggcct
     2221 accttctggc ggtcggcgat ctactgatcg gctggctgtt gctcgagcac gccgagatcg
     2281 cgctgaccgc cctggatggc gatacgtcgg accgggaccg gtccttttat cgggggaagg
     2341 tggcggcggc ggagttcttc gcgcgtaatg tgttgccgcg gttggccgcc gaccgcaaaa
     2401 tcgtcgaggg cgtggacctg acgatcatgg acctacgaga ggaagcattc tgatgaaggt
     2461 gatcacctcg atcgacgatg ccgtcgccac ggtgggggag gaactcggcg tcggcgagtg
     2521 gcaggaggtc gaccaggacc gcatcaacgc cttcgcggat gcgaccggcg atcatcagtg
     2581 gattcacatc gacgccgagc gcgcgaaaac ccagagtcct tacggccaga cgattgcgca
     2641 cggctacctg acgctgtcga tgatccccgc gctcaccaag gacaactacc gcgtcgagaa
     2701 cgcgaagatg gcgatcaact acggctcgaa caaggtgcgc ttcatcgcag ctgtgccgtc
     2761 gggcggccgg atccgggcgc gctccgaact catcgatgcg accaaggtcg acgacaacac
     2821 cgtcaacctg acggtgaaga acaccatcga actcgacggg tcgaacaagc ccgccgcggt
     2881 ggccgagacg atcgtgcggg tgctgttcta accaacgggg cgaccgtcgt gagtagcggc
     2941 tccagaccgc cgaacatcgt gtttggtaca agggctttcg tggacggatc cagcgcgcga
     3001 tggcgcagcg ggccggcgcc accaccgcag ccgcacgctg ttgccgcctt cattcgacag
     3061 gcctgcatct aggagatggc gttcgttggc ggcttcagtg cgttcaggac tgtggcattt
     3121 gcgagagtga aatctgcccg ggttgcccag atggggtgct cggcgaagtc gcgtacggat
     3181 tgaggcatat tgccttcagc ggcatgaccc gccagaagaa tcgcggtccc taaaccaatg
     3241 accgcagttc cgaggcgcgg ccgacccgaa agcagcgcaa ctgcagtcgg gacaatcgaa
     3301 tagttgccga cgacgccaca ccaccggttg aacgcggtca ggtgctgagc acgatgttca
     3361 gctgcgaatt ccccgtagga ggcaaccatg tctcgagcat cccaccaaaa aaa
//
LOCUS       DASYLX010000251         6556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_339158,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000251 DASYLX010000000
VERSION     DASYLX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339158"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGP27_06215"
     CDS             complement(<1..738)
                     /locus_tag="VGP27_06215"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV7850943.1"
                     /translation="MALVVQKYGGSSVSDAERIRRVAERIVETKKQGNDVVVVVSAMG
                     DTTDDLLDLARQVCPAPPAREMDMLLTAGERISNALVAMAIDSLGAQARSFTGSQAGV
                     ITTGTHGNAKIIDVTPGRLRSALDEGLIVLVAGFQGVSQDSKDVTTLGRGGSDTTAVA
                     LAAALKADVCEIYTDVDGIFSADPRIVPNARHLDTVSFEEMLEMAAAGAKVLMLRCVE
                     YARRYDVPIHVRSSYTDKPGTLVKGSME"
     gene            825..1427
                     /locus_tag="VGP27_06220"
     CDS             825..1427
                     /locus_tag="VGP27_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7850944.1"
                     /translation="MGRKPAKKVAETRVPIHPDIAARWSPRMFDPDAVLDDEQLVAVL
                     EAARWAATWGHRQPVRFIVGLRGDGTFADITGLLKRGNRYAHAAGALVVVCIDEGEDE
                     RTARYAAVDAGAAIAQLTIEAVSRGLVAHPMAGFDADGACQVFGIPAGVRPVAVVAIG
                     SLGDYDNAPPEILERDAKGRSRLPLNEVVFAGHWGRPLSL"
     gene            complement(1440..1895)
                     /locus_tag="VGP27_06225"
     CDS             complement(1440..1895)
                     /locus_tag="VGP27_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850945.1"
                     /translation="MKLPRRWTFTIIAVVVAVIAFALSYFGVFGPSSEECKPVNDLLA
                     FNKSQAEQIAAKTGDSEGVPDIAEEGAYQAWADGLAERAQNVHQPDLARDATQLASLS
                     NEFVGKFSGLRSEAQSRAPGAPAPPAVFQMDLLNSQIADTIKKLSDACG"
     gene            2031..3284
                     /locus_tag="VGP27_06230"
     CDS             2031..3284
                     /locus_tag="VGP27_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV7850946.1"
                     /translation="MDTGTTAFMLCCIIGLTLMIPGLALFYGGMVSVKSSTNMMMMTF
                     GAVAIVGVLWVLFGFSMTFGTSYGGFVGSFTEFLGMKDLLKPDTTISGLPITLFALFQ
                     ALFAAITVALISGAVADRMKFGAWMIFAAVWAILVYFPVAHWVFAFDGVVTENAVGGW
                     IANSLKAIDFAGGTAVHINAGAAALAVAIVLGKSAGWGKLRKPHNVPLTLLGAGLLWA
                     GWYAFNGGSALAAGNSAAIVMVTTFVATCAATLAWIAVEKVKDGHVTGVGAASGAITG
                     LVAITPACGAVTPVGAIILGAIAGVVCIYAVGLKARFGYDDSLDVVGVHLVGGVVGTL
                     LIGLLASESMPNATNGLFYGGGFDQLWRQAVAAGAVMVYSFVVAYIIAFAIKKTMGIR
                     ISPDEEETGIDAAFHREASYELAPA"
     gene            3392..4699
                     /gene="glnT"
                     /locus_tag="VGP27_06235"
     CDS             3392..4699
                     /gene="glnT"
                     /locus_tag="VGP27_06235"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEV7850947.1"
                     /translation="MSTDLAALAHESGTKFILALFVDLRGKPCAKLVPVEAVDLLATE
                     GVGFAGYAVGAIGQEPKDPDLMAIPDPASFTSIPFIKDGLAIVHCDPHVNGEPWPYAP
                     RVILKSLIQRAADAGFEPWVGAEVEYFLLHRNTDGSLSVADAADTAAQPCYDARGVTR
                     MYDHLTAISAAMNQLGWSNYANDHEDGNGQFEQNFQFSDALTTADRVVTLRYLLSMIA
                     AERGMVATFMPKPFAEKTGSGLHLHMSLTSGSTPVFPADVDERGLGLSDTAYNFVGGI
                     LEHACALQAVVAPTVNSYKRTGATSTSSGASWAPRMPTYGGNDRTHYIRVPDNARIEL
                     RGGDGSANPYLAIAAALGAGIDGIKRSADPGPVGGGSSGRPLPPTLLHAVDEFEADSV
                     ITGVLDAAGDGVAAYFANVKRDEFFAYHSAVAPWEIDNYLTAF"
     gene            4727..5623
                     /locus_tag="VGP27_06240"
     CDS             4727..5623
                     /locus_tag="VGP27_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV7850948.1"
                     /translation="MCGIVGLHLRSPQLYPRLGELLTGMLGEMGNRGSDSAGVAVYGD
                     PVWSPPGRGCVSVVDVSEDADIAAAAVSAHLGSDVDIAAVDNTYLLSADATSAVLLDA
                     VKAAYPQALIAGFGADMAVLKGVGHPRTLTDAWGLAKAQGWQGVGHTRMATESAVTPA
                     GCHPYTVGPEQCMVHNGSFANHATIRRQLRSAGIEFDSENDTEVGARFIAKQLADGRD
                     VESALKELCATFDGFYTLLVSNRDSFAVVRDAIACKPAVIAETDDWVAVGSEFRALAG
                     LPGVDSATIWEPEPEVIYAWNR"
     gene            5611..6315
                     /locus_tag="VGP27_06245"
     CDS             5611..6315
                     /locus_tag="VGP27_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HEV7850949.1"
                     /translation="MEQVTATVSSRLFDLRKTKLREVNAALHATDLSGEFAIIHPDGA
                     HNVAVGVNAPVKVGVEGHVGYYAAGMNQQAEIIIDGNAGTGVAENMMSGSVWVKGDTS
                     QSAGASAHGGLLVVDGNAAARCGISMKGVDIVIGGNVGHMSAFMAQAGRLVIRGDAGE
                     ALGDSIYEARIYVRGEVASLGADCVAKEMGREHHEELEYLLKTAGFDDDDTSAYTRYG
                     SARALYHFHVDNASSY"
     gene            6315..>6556
                     /locus_tag="VGP27_06250"
     CDS             6315..>6556
                     /locus_tag="VGP27_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEV7850950.1"
                     /translation="MNHTTDDRARLGLRESATFDRQTIAAIHRAAETGIYDIRGWGAK
                     RPLPHFDDLLFLGASMSRYPLEGYRERCGTDVVLGDR"
BASE COUNT         1078 a         2176 c         2187 g         1115 t
ORIGIN      
        1 ctccatcgat cctttgacga gcgtgccggg cttgtcggtg tatgacgacc ggacgtgaat
       61 cggaacgtca taccggcggg cgtattccac gcagcgcagc atcagcacct tggccccggc
      121 cgcggccatc tcgagcatct cttcgaagga cacggtgtcg aggtgccgcg cattcggcac
      181 gatccgcggg tcggcgctga agatgccgtc cacgtccgtg tagatctcgc acacgtcggc
      241 cttcagcgcg gcagccagtg ccacggcggt ggtatccgaa ccgccccgac cgagcgtggt
      301 gacgtccttg ctgtcctggc tgacgccctg aaagccggcg acgagcacga tcaacccctc
      361 gtcgagcgcg gagcgcagcc ggccgggcgt gacgtcgatg atcttggcgt tgccgtgggt
      421 gccggtggtg atcacacccg cctgcgaccc ggtgaaggat cgggcctggg cgccgagcga
      481 gtcgatggcc atcgcgacca gcgcgttgga gattcgctca cccgcggtga gcagcatgtc
      541 catctcccgc gcgggcgggg ccgggcagac ctgtctggcc aggtcgagca ggtcatcggt
      601 ggtgtcaccc atcgccgaga ccacgacgac gacgtcgttg ccctgcttct tggtttccac
      661 gatgcgctca gcgacgcggc ggatccgttc ggcgtcagaa accgacgatc cgccgtattt
      721 ctgcacgacg agcgccactg tcggaccttt cgggtgttgt ggaagatccc accaaggata
      781 aggggtgtgc gcacctcatt tacctcgccg gtagcgtcac ggccgtgggc cgcaagccag
      841 ccaagaaagt cgccgagacc cgggtgccga tccacccgga catcgccgcg cggtggagtc
      901 cacgcatgtt cgacccggat gccgtgctgg acgacgagca gttggtcgcc gtcctcgagg
      961 ccgcgcggtg ggcggcgacg tggggacacc ggcagccggt ccgcttcatc gtcgggttac
     1021 gtggcgacgg gaccttcgcc gacatcaccg ggctgctcaa gcgcggcaac cgctacgcgc
     1081 atgccgcggg cgcactcgtc gtggtgtgca tcgatgaggg tgaggacgag cgcaccgccc
     1141 gctacgcggc ggtcgacgcg ggcgcggcga tcgcacagct gaccatcgag gcggtgtcgc
     1201 gcggtttggt cgcgcacccg atggcggggt tcgacgccga cggcgcctgt caggtattcg
     1261 ggatcccggc gggcgtgcgt ccggttgcgg tggtcgcgat cggttcgctc ggcgactacg
     1321 acaacgcgcc gccggagatc ctcgagcgcg acgcgaaggg ccgcagccgg ctgccgttga
     1381 acgaggtcgt gttcgcgggc cactggggcc ggccgctgtc gttgtgacca cgacccgggt
     1441 cacccgcagg cgtcggacaa cttcttgatg gtgtcggcga tctggctgtt cagcagatcc
     1501 atctggaaca ccgcgggggg cgcgggcgca cctggcgcac gtgattgggc ttcgctgcgg
     1561 agcccggaaa acttcccgac gaactcgttg gacagactgg cgagctgggt ggcgtcgcgt
     1621 gccaggtcgg gttggtgcac gttctgggca cgttcggcca ggccgtcggc ccaggcttgg
     1681 taagcgccct cctcggcgat gtcgggaact ccttcggagt ctccggtctt ggccgcgatc
     1741 tgctcggcct gcgacttgtt gaaggcgagc aggtcgttga ccggcttgca ctcctccgac
     1801 gacggaccga acacgccgaa gtacgagagt gcgaaggcaa taacggcgac gaccaccgcg
     1861 atgatcgtga acgtccaccg ccgagggagc ttcatcgcag gcaactctaa cgggagcccg
     1921 actattaaca ctcacgcaac agcgcgaacc cgtccacgta acagaaacat cggttactgc
     1981 cagtgatcgg ccgtccgaag gccgcaatca ctcaggagat gtatatggac gtggatacag
     2041 gaaccaccgc attcatgctg tgttgcatca tcggcctcac gctgatgatt cccgggctcg
     2101 cgctgttcta cggcggcatg gtgtccgtca agagctcgac caacatgatg atgatgacgt
     2161 tcggggccgt cgcgatcgtt ggcgtgctgt gggtgctgtt cggtttctcg atgaccttcg
     2221 gcacttccta cggcggtttc gtcgggagct tcaccgagtt cctgggcatg aaggaccttt
     2281 tgaaaccgga caccacgatc agcgggctgc cgataacttt gttcgccttg ttccaggccc
     2341 tgttcgcggc gatcaccgtg gccctcatct cgggtgcggt ggccgatcgg atgaaattcg
     2401 gcgcgtggat gatcttcgcg gcggtgtggg cgatccttgt ctacttcccg gtcgcgcact
     2461 gggtcttcgc cttcgacggt gtggtcaccg agaacgccgt cggcggttgg atcgccaata
     2521 gcctgaaagc catcgacttc gccggtggca ccgcggtgca catcaacgcc ggcgcggccg
     2581 cgctggccgt cgccatcgtg ctgggcaagt ccgccggatg gggcaagctg cgcaagcccc
     2641 acaacgtgcc gctgacgctg ctcggtgcgg gcctgctgtg ggccggctgg tacgccttca
     2701 acggcggttc ggcgctggcc gccgggaact ccgccgcgat cgtcatggtc accacgttcg
     2761 tcgccacctg tgcggcgacg ctggcctgga tcgcggtgga aaaggtcaag gacggccacg
     2821 tcaccggcgt cggtgcggcg tccggcgcaa tcaccgggct ggtcgcgatc acacccgcct
     2881 gcggtgcggt cacgcccgtc ggagcgatca tcctcggtgc gatcgcaggc gtcgtctgca
     2941 tctacgcggt cgggctcaag gcgcgcttcg ggtatgacga ctcgctcgac gtggtcggcg
     3001 tccacctggt gggcggcgtc gtcggcacgc tgctgatcgg cttgctggcc agcgagagca
     3061 tgcccaacgc caccaacggc ctgttctatg gcggcggttt cgaccagctg tggcggcagg
     3121 cggtcgctgc gggtgcggtg atggtctact cgttcgtcgt cgcctacatc atcgccttcg
     3181 cgatcaagaa gacgatgggc atccgcatct cgcccgacga agaagaaacg ggtatcgacg
     3241 cggccttcca ccgcgaagcg tcgtacgagc tagctccagc ctgacccaag ctccggccgg
     3301 cgcggaccca ctcttccgcg ccggccggtt ggtcggcacc gtgtttcata tggcgataca
     3361 atgatcactc acaagagaca gggcttccca catgtccacc gacctcgccg cactggctca
     3421 cgagtcagga acgaagttca tcctcgcgct gttcgtcgac ctgcggggaa agccgtgcgc
     3481 gaaactcgtt cccgtcgagg ccgtcgacct gctcgccacc gagggtgtcg gcttcgcggg
     3541 ctacgccgtc ggcgcgatcg gccaggagcc caaagaccct gacctgatgg ccattccgga
     3601 tccggcgtcg ttcacgtcga tcccgttcat caaggacggc cttgccatcg tccactgcga
     3661 cccgcacgtc aacggtgagc cgtggcccta cgcaccgcgg gtgattctca agtcactgat
     3721 tcagcgcgcc gccgacgccg gcttcgagcc gtgggtgggc gccgaagtcg agtatttcct
     3781 tctgcaccgc aacaccgatg gcagcctttc ggtcgccgac gccgccgata ccgccgccca
     3841 gccttgctac gacgccaggg gcgtcacccg gatgtatgac cacctgacgg cgatctccgc
     3901 ggcgatgaac cagctgggct ggtccaacta cgccaacgac cacgaggacg gcaacggcca
     3961 gttcgagcag aacttccaat tctccgatgc cctgacgacc gccgatcgtg ttgtgacact
     4021 gcgttatctg ctgtcgatga tcgccgccga gcgcggcatg gtcgcgacct tcatgccgaa
     4081 gccgttcgcc gagaagaccg gcagcggact gcatctgcat atgtcgctca ccagcggcag
     4141 cacgccggtc ttccccgccg acgtcgacga gcgcggcctc gggctctcgg acaccgcgta
     4201 caacttcgtc ggcggaatcc tcgagcatgc ctgcgctctg caggcggtgg tggccccaac
     4261 ggtcaactcc tacaagcgaa ccggagcgac gagcacatcc tcgggcgcat cgtgggcgcc
     4321 gaggatgccg acgtacggcg gcaatgaccg cacccattac atccgggtgc ccgacaatgc
     4381 acgcatcgaa ctgcggggcg gcgacggttc ggcgaacccg tacctcgcca tcgccgcggc
     4441 actgggcgcg ggcatcgacg ggatcaagcg cagcgcagat cccggccccg tcggcggggg
     4501 ctcgtcgggc cggccgctgc cgccgacgct gctgcacgcg gtcgacgagt tcgaggccga
     4561 ttcggtgatc accggcgtcc tcgacgccgc gggcgacggc gtcgccgcct acttcgcgaa
     4621 cgtcaaacgc gacgagttct tcgcctacca cagcgccgtt gcgccctggg agatcgacaa
     4681 ctacctgacc gccttctgaa cgacgagaca tcaaaggagt caacccatgt gcgggatcgt
     4741 cgggttgcat ctgcggagcc cgcagctgta ccccagactc ggggaactgc tcaccgggat
     4801 gctgggtgag atgggcaacc gcggcagcga ctcggccggc gtcgccgtct acggcgatcc
     4861 ggtctggtcg cctcccggcc gcggttgtgt gtcggtggtc gacgtctccg aggacgcgga
     4921 catcgccgcc gccgctgtca gcgcgcacct cggcagcgac gtcgacatcg ccgcggtgga
     4981 caacacgtat ctcttgtcgg ccgacgcgac atccgcggtg ctgctcgacg ccgtcaaggc
     5041 ggcgtatcca caggcgctca tcgccgggtt cggcgcggac atggccgttc tcaagggcgt
     5101 cgggcacccg cgcacgctga ccgatgcgtg ggggctggcc aaggcacagg gctggcaggg
     5161 agtcggacac acccggatgg cgaccgaatc ggcggtcacg ccggcgggct gccaccccta
     5221 cacggtcggg cccgagcagt gcatggtcca caacggctcg ttcgcgaacc atgccaccat
     5281 ccgcaggcag ttgcgttccg cgggaatcga attcgacagt gagaacgaca ccgaggtcgg
     5341 cgcgaggttc atcgccaagc agttggccga cggccgtgac gtcgagtcgg cgctgaagga
     5401 actgtgcgcg acgttcgacg gcttctacac gctgctggtg tccaaccggg actcgttcgc
     5461 ggtggtccgc gatgcgatcg cctgcaagcc cgcggtgatc gccgagaccg atgactgggt
     5521 cgccgtcggc agcgaattcc gcgcgctcgc cggacttccc ggtgtggaca gcgccaccat
     5581 ctgggagccg gagcctgagg tgatctacgc atggaacagg taacagccac ggtgtcatca
     5641 cggttgttcg atctgcgcaa gactaaattg cgggaggtca acgcggcgtt gcacgccacc
     5701 gacctgtccg gtgaattcgc catcatccat cccgacggcg cccacaacgt cgccgtcggt
     5761 gtgaacgcac cggtgaaggt cggcgtggag ggccacgtcg ggtactacgc cgcggggatg
     5821 aaccagcagg ctgagatcat tatcgacggg aacgcgggca ccggcgtcgc cgagaacatg
     5881 atgagcggct cggtgtgggt gaagggcgac acctcccaat cggcgggtgc cagcgctcat
     5941 ggcgggctgc tcgtcgtcga cggcaatgcc gctgcgcgct gcggcatttc gatgaaaggc
     6001 gtcgacatcg tgatcggcgg caacgtcggt cacatgagcg ccttcatggc gcaggcggga
     6061 cggctggtca tccgcggcga cgcgggcgag gcactgggag actcgatcta tgaggcccgg
     6121 atctacgttc gcggagaggt ggcgtccctc ggcgccgact gtgtcgccaa ggaaatgggc
     6181 cgcgaacatc acgaggagct cgaatacctc ttgaagaccg ccggtttcga cgacgacgac
     6241 accagcgcct acacgcggta cggctcggcg cgcgccctct accatttcca cgtcgacaac
     6301 gcttcgagct actgatgaac cacaccaccg atgatcgggc gcgcctcggc ctgcgtgaat
     6361 ccgccacctt cgaccgccag acgatcgcgg ccatccaccg agcggccgag accggcatct
     6421 acgacatccg cggctgggga gccaagcgac ccctgcccca cttcgacgac ctgctcttcc
     6481 tcggcgcatc gatgtcgcgc tacccactgg agggctaccg cgagcgctgc ggcaccgatg
     6541 tcgtcctcgg tgatcg
//
LOCUS       DASYLX010000252         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1656436,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000252 DASYLX010000000
VERSION     DASYLX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656436"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGP27_06255"
     CDS             <1..190
                     /locus_tag="VGP27_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850951.1"
                     /translation="SLSQFQTDVSDHRIGYYIAPDTSHGPGRGRQHTDITRWVAANFA
                     AVKAGSATVYDLGAPLAR"
     gene            complement(229..1053)
                     /locus_tag="VGP27_06260"
     CDS             complement(229..1053)
                     /locus_tag="VGP27_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV7850952.1"
                     /translation="MSGTTQRELGDSDSPIEDELEDAFNRMIAEGTQRLHRPWREVLT
                     TGFFGGTEVAMGVLAYLSVLAATHDPLVAGLAFSVGFLALLLGRSELFTEGFLVPVTT
                     VAAKRASVGQLVKLWSGTLAANLAGGWVIMWLVMTALPKLRTQTIESARHFIEAPLSA
                     ESLSLAVLGGMVITLMTRMQHGTDSVPGKIAAAIAGAFLLAGLQMFHSILDSLLIFGA
                     LISGKAPFGYVDWLAWFGYTLAGNIAGGLVLVTLLRLLRSKDRLLQERDQAQQSPD"
     gene            complement(1155..2501)
                     /locus_tag="VGP27_06265"
     CDS             complement(1155..2501)
                     /locus_tag="VGP27_06265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7850953.1"
                     /translation="MPLAVGDVFAGYTVVRTLGSGGMGEVYLVDHPRLPRRDALKVLP
                     AELTSDSEYRQRFNREADIVAHLWHPDIVSVHDRGETDGQLWLTMDYVEGTDAARLLQ
                     DYRGGGMPPGDVVRLVSAVADALDYAHQRQLLHRDVKPSNILLSAAESGRQRILLADF
                     GIARWVDDSGGLTATDMTVGTVAYAAPEQLLGDALDGRADQYALAATAFHLLTGAQPF
                     QHTNPAVVISQHLTAPPPAIADRVPELSRLGPVFSRALAKKPEDRYPSCMAFADALKE
                     SLDGVSPEVVSGDATMPAARARRAPVTTSRKRAFRPVSLAVALAALVGVAALIGLLLG
                     DRHRSNTPAQPQPAPPTSAVIPVVLIGADCATLGAEGVTSAGQTAYCARLPTTGDIMW
                     SIYSGRVPTPSDAPGPNDPVYPAGIEDQVRVCVAQTQQSRVKCRDDVRQGNLYGPA"
BASE COUNT          476 a          873 c          878 g          435 t
ORIGIN      
        1 ctctctttct cagtttcaga ccgatgtcag tgatcacagg atcggctact acatcgcacc
       61 cgacacctca cacggtcccg gccgcggtcg ccagcacacc gacatcacgc gttgggtcgc
      121 ggcgaacttc gcggcggtca aggccgggtc tgccaccgtc tacgacctcg gggcgccgct
      181 tgcaaggtga gaagccgctc gcaaggtgag aacaggaacg cgtggcgctc aatcgggcga
      241 ctgctgcgcc tggtcgcgtt cctgcagcag acggtccttg ctacggagta ggcgcaacag
      301 ggtgaccaag acgagtccac cggcaatgtt gcccgcgagt gtgtatccga accaggccag
      361 ccagtcgacg tacccgaacg gcgccttccc ggatatcaga gcgccgaaga tcaacagcga
      421 gtcaaggatt gagtggaaca tctgcagacc ggccagcaga aaggcgccgg cgatcgcggc
      481 cgcgatcttg cccggcactg aatcggtgcc atgctgcatc cgggtcatca gcgtgataac
      541 cattccgccg aggacggcca gcgacaacga ttctgccgac aggggtgcct cgatgaagtg
      601 tcgcgccgat tcgatcgtct gtgtccgcaa cttcggcaac gcggtcatca ccagccacat
      661 gatcacccag ccaccggcca ggttggccgc cagtgtgccg ctccacagct tgaccaactg
      721 ccccacactg gcacgtttgg cggccaccgt ggtcaccggc accagaaacc cctccgtgaa
      781 caactcgctg cggccaagca gaagtgcgag aaagccgacg gagaacgcca gcccggccac
      841 cagcgggtca tgcgtcgccg caagcaccga caagtacgcc agcacgccca tcgcgacctc
      901 ggtgccaccg aagaaacctg tggtcagcac ctcgcgccag ggccggtgga ggcgttgcgt
      961 gccctcggcg atcatccggt tgaacgcgtc ctcgagctcg tcttcgatcg ggctgtcgga
     1021 atcgccgagc tcgcgttggg tggttccgct cacgacaaag tcctcgcctc gaccatggtt
     1081 gaagccactg tgtacccggg cggcgaaagc cccaagcgca ctgtcagctg tcgcaacagg
     1141 ctcgcaacgt cggtctacgc cgggccgtac aggtttccct gccggacgtc gtcacggcac
     1201 ttgacgcggc tctgctgggt ttgcgcgaca caaacacgaa cctggtcctc gattccggcc
     1261 ggatacaccg ggtcgttggg gccgggtgca tcgctggggg tggggacccg acccgaatag
     1321 atcgaccaca tgatgtcgcc ggtggtgggc agacgcgcgc agtaggcggt ctgcccggcc
     1381 gaggtaactc cttcagcgcc gagggtggcg cagtccgctc cgatcaagac gaccgggatg
     1441 acggccgacg tcggtggtgc cggttgcggt tgcgccgggg tgttggagcg gtgtcgatcg
     1501 ccgagtagca ggccgatcag cgcggccacg ccgaccagcg ccgcgagagc gacggccagg
     1561 ctcaccggac gaaaagcccg ctttcgactc gtggtcaccg gtgcgcgtcg cgctctggcc
     1621 gccggcatgg tggcatcgcc tgagacgact tcgggcgaga cgccgtcgag actctccttc
     1681 agcgcatcgg cgaatgccat gcaggagggg taacggtcct ccggtttctt ggccagggcg
     1741 cgggagaaca ccggacctag tcgggacagt tcgggaaccc ggtcggcgat ggccggcggc
     1801 ggcgcggtca gatgctggct gatgacgacg gccgggttgg tgtgttggaa gggctgcgct
     1861 ccggtgagca gatggaatgc cgtcgccgcg agcgcatatt ggtcggccct gccgtccaag
     1921 gcatcgccaa ggagttgttc gggcgcggca taagcgaccg ttcccacggt catgtcggtc
     1981 gccgtaaggc cacccgagtc gtcgacccat cgcgcgatcc cgaagtcggc gagcaagatc
     2041 cgctgccgcc cggattcggc ggcggacagc aggatattgg acggcttgac gtcgcgatgc
     2101 agaagctggc gctgatgcgc gtaatccagc gcatcggcga cggcggacac caggcgtacc
     2161 acgtcgccgg gcggcattcc gccgccgcgg tagtcctgca ggagccgggc tgcgtcggtg
     2221 ccctctacgt aatccatcgt gagccacagc tggccgtcgg tttcgccgcg gtcgtgcacg
     2281 ctgacgatgt ccggatgcca gaggtgcgcg acgatatcgg cttcccggtt gaaccgttgc
     2341 cggtattcgc tgtcgctggt gagctccgcg ggcagcactt tgagtgcatc ccgacgcggc
     2401 agccgcggat gatcgacgag atacacctcg cccatgccgc cggatccgag ggtgcgcacg
     2461 acggtgtagc cggcgaatac gtcgccgacc gctagcggca ttgccccgcc tgatcagacc
     2521 tgcgccacat ctcgcggtta tcgcctgttt gccgaatatc gaaacctccc aatgggagca
     2581 cgaccttatc gcgcgaacct ggaacagccg cacacgagcg cccgtgtcaa gctggacgaa
     2641 ggatcggttt cctggctaac cc
//
LOCUS       DASYLX010000253         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1326478,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000253 DASYLX010000000
VERSION     DASYLX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326478"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VGP27_06270"
     CDS             complement(<1..150)
                     /locus_tag="VGP27_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEV7850954.1"
                     /translation="MRRADGKPINVLVVDDEPVLAELVSMALRYEGWEIATAGDGATA
                     IALAKD"
     gene            343..858
                     /locus_tag="VGP27_06275"
     CDS             343..858
                     /locus_tag="VGP27_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850955.1"
                     /translation="MTSEIAGKPPSRRWATRPTLAAVAVAVAVGGVGGAAVYAATGSS
                     SHSMGGGMRGPGGPGAPVHGQPPGQGALLPPAAQGTPGSVLHSEYVVSDGHGGFTTKM
                     TQTGTVDEVTLSSIVVRSDDGYTQIYVFPSAAAAPTKPVAADDTVTVQATRTGPTVTL
                     NGIGEGPPRGN"
     gene            complement(875..1087)
                     /locus_tag="VGP27_06280"
     CDS             complement(875..1087)
                     /locus_tag="VGP27_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850956.1"
                     /translation="MISAAVGASAVAGMAALTVALSTDSGSATVVSNPQMTLGETSTI
                     EKAATVLETSMAVPEVTAEVPDGFGP"
     gene            complement(1147..1350)
                     /locus_tag="VGP27_06285"
     CDS             complement(1147..1350)
                     /locus_tag="VGP27_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850957.1"
                     /translation="MSALGVASSNVSAAEEPTPAPPGPVTTSEITTGATVTDMTAMEG
                     PAAAVAVPPITTPPSSFGQEPPA"
     gene            complement(1560..1817)
                     /locus_tag="VGP27_06290"
     CDS             complement(1560..1817)
                     /locus_tag="VGP27_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850958.1"
                     /translation="MNSIPRNQAKLLAAVLGGGAMVTMGALTMSIHQQPTLSQNVAVG
                     QMTVAATTTASGKDDTVEATSMAVPSIKGPAPLPPEEKAAE"
     gene            2024..3001
                     /locus_tag="VGP27_06295"
     CDS             2024..3001
                     /locus_tag="VGP27_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850959.1"
                     /translation="MTRFLTRRLLNYIVLLILASFLTFVLTSVSFKPLDSLASRNPRP
                     PQTVIDAKAAELNLDRPIPIRYVRWASGAIRGDFGTTVTGQPISDELGRRVGVSLRLL
                     IIGAVLGTVIGVVVGAWGAIRQYHLSDRVITVLSLLLLSTPVFVTANLLILGANGVNS
                     VLDVQVFEYIGETSPDALTGGWSQFVDRLQHLVLPTFTLALMGIAGYSRYQRNAMLDV
                     LGQDFIRTARAKGLTRRQALFKHGLRTALIPMATLFAYGISGLVTGAVFTEKIFGWHG
                     MGEWVVQGIATQDTNIIAAVTVFTGAAVLFAGLLSDVIYAALDPRVRVS"
     gene            3046..>3609
                     /locus_tag="VGP27_06300"
     CDS             3046..>3609
                     /locus_tag="VGP27_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7850960.1"
                     /translation="MDAHAFASRRTLVWRRFLRSKPAVASLIMLVLFFVGCYALPPLL
                     PYSYTDLDFYALQQPPNPSHWFGTNALGQDLLALTLRGMQKSMLIGVCVAIISTVLAA
                     TVGAIAGYFGGWRDRRLMWLVDLLLVVPSFIVIAIVTPRLGSADRVFWLILLFSVFGW
                     MISSRIVRGMTMSLREREFVVAARYMGV"
BASE COUNT          575 a         1184 c         1148 g          702 t
ORIGIN      
        1 gtccttggcc aacgcgatcg cggtggcgcc atcgcccgcg gtggcgatct cccagccctc
       61 ataacgcagt gccatcgaca ccaactcggc gaggacgggc tcatcgtcga cgacaaggac
      121 gttgatcggc ttgccgtccg cgcggcgcat tacgacacgc tcgggatcgc tcgcgttcgc
      181 gctcacagag atccgttcga attacgcacg tcagtcatta ttgtctcggc ccgttagcgc
      241 gcactgtgcc gactgtgtgt gccggctgtg aagaggtccg catcgatcga accctgatga
      301 cactcacagc ccacacacat gtcaccgctg cacagtggtc gggtgacgtc ggagatcgca
      361 ggaaagccgc cctctcgtcg gtgggccacc cgtcccacgc tggcagccgt cgccgtggca
      421 gtggcggtcg gcggggtggg cggcgccgcc gtttacgccg cgaccggtag ttcgtcgcac
      481 tcaatgggcg gtgggatgcg cggcccgggc ggtccaggag cccctgtgca cggtcagccg
      541 ccgggacagg gagccctgct accgcccgcg gcgcaaggga cacccgggag cgtcctacac
      601 agtgagtacg tggtgtccga cggccacggc gggttcacca ccaagatgac gcagacgggc
      661 accgtcgacg aggtgactct gtcgtcgatc gtggtgcgca gcgacgacgg ctacacgcag
      721 atctatgtgt ttccgtcggc ggccgccgcg ccgaccaagc cggtggccgc cgacgacacg
      781 gtcacggtgc aggccactcg aaccggtccg acggtgaccc tcaacggcat cggtgagggc
      841 ccaccgcgcg ggaattaacc ggcgccggtt gcgattacgg cccgaagccg tcaggcacct
      901 cggcggtcac ctctggcacc gccatcgacg tctcgaggac ggtcgcagcc ttctcgatgg
      961 tgctcgtctc gcccagggtc atctgcggat tggaaacgac agtggccgag ccgctgtcag
     1021 tgctcagcgc cactgtcaga gccgccattc cggccaccgc gctcgcgccg acagccgctg
     1081 aaatcacctt caatctgtgc gaattcatct catacctctc gtaggtttac gtaaaagagc
     1141 cgactgtcag gcgggcggtt cctgcccgaa cgacgagggc ggggtggtta tcgggggcac
     1201 ggcgaccgcc gccgcgggac cctccatcgc cgtcatatcg gtgacggttg cacctgtggt
     1261 gatctctgac gtggtcaccg ggcccggcgg cgcaggcgtc ggctcctcag cggcgctgac
     1321 gttgctggac gccactccca gcgcactcat ggcaaccacg gcggcggccc cgaacccgcc
     1381 gaaaccatct tgaactgttt cgctctcacc tctgaccact ccatttcggt gtctggtcag
     1441 ccgccccatc cagcccggac ggccggtcga acagtgcctc gaggggtcgc cgacgacccc
     1501 tccggcatat tgcataaccg gaagggtcgg aggtgaaacg tcagtcggcg aggaagccgc
     1561 tattcggcgg ctttctcctc ggggggcagc ggcgccgggc ccttgatcga gggtacggcc
     1621 atcgacgtcg cctcgaccgt gtcgtcctta cctgaggccg tcgtcgttgc ggccacggtc
     1681 atttggccca ccgcgacgtt ctggctgagg gtgggttgct gatggatgct catggtcagc
     1741 gcgcccatcg tcaccatcgc gccgccaccg agcaccgcgg ccagcagttt cgcctggttc
     1801 cgaggaattg aattcatttt catactccag ttcatggtcg ttgccgactc gcccctgcgg
     1861 tgcggcgaac cctgaaaatg caggtgagaa ccagtgaact cgcggtttct gtagctaggc
     1921 ttaagaaccg gtaggaattg actagccgcc ccgccgtccc gttcgcagcc ccaccggcgg
     1981 gcatctcata ccctgtgcgc atgcttgcaa tggaacctcg ccgatgacgc ggttcctgac
     2041 gcggcgtctt ctcaactaca tcgtgctgtt gatcttggcg tcgttcctga cattcgtgtt
     2101 gacctcggtg tcgttcaagc cgctggacag cctcgcgtca cgcaaccccc gtccgcccca
     2161 gacggtgatc gacgcgaagg cggccgagtt gaacctggat cggccgattc cgatccgcta
     2221 cgttcgctgg gcatcgggtg ccattcgagg cgacttcggc acaaccgtga cgggccaacc
     2281 catctccgac gaattgggtc gccgcgtcgg ggtcagcctg cgacttctga tcatcggtgc
     2341 ggtgttgggg acggtcatcg gtgtggtcgt cggcgcctgg ggggcgatcc ggcagtatca
     2401 cctcagcgac cgcgtcatca cggtgctgtc gttgttgctg ttgagcaccc cggtgttcgt
     2461 caccgcaaac ctgttgatcc tgggcgcaaa cggagtgaat tcggttctcg acgttcaggt
     2521 gttcgaatac atcggtgaga cgtcaccgga tgcgctcacc ggcggatgga gccagttcgt
     2581 cgaccggcta cagcacctgg tgctgccgac gttcacgctc gcactgatgg gaatagccgg
     2641 ctacagccga tatcagcgca atgcgatgct cgatgtgctc ggtcaggatt tcatccgcac
     2701 cgcgcgcgcc aagggcttga cccgcaggca ggcactgttc aaacacggcc tgcgcaccgc
     2761 tctgattcca atggcgacgc tgttcgccta tggcataagc ggtttggtga ccggggcggt
     2821 gttcaccgaa aagattttcg gctggcacgg catgggcgag tgggtagtgc aggggatcgc
     2881 gacccaggac accaacatca tcgccgccgt gaccgtgttc accggcgctg ccgtcctctt
     2941 cgccggcctg ctctccgacg tcatctacgc cgcgctggat cccagggtgc gtgtgtcgtg
     3001 acagagccgg tgaccaccga aaccgcgacg acagccaagt ccgggttgga cgcgcacgca
     3061 ttcgcttccc gccgaaccct ggtctggcgc aggttcttac gcagcaagcc cgccgtcgca
     3121 tcgctgataa tgctcgtgct gttcttcgtc ggctgctacg cgctgccacc gctgctgcct
     3181 tacagctata ccgacctgga cttttacgcg ctgcagcagc cgcccaatcc cagccactgg
     3241 ttcggcacca acgcgctcgg ccaggatcta ctggcgctga cgcttcgcgg catgcagaag
     3301 tcgatgttga tcggcgtctg tgtggccatc atctccacgg ttctggccgc gacggtcggt
     3361 gcgatcgccg gctacttcgg tggctggcgc gatcgcaggc tgatgtggct cgtcgatctc
     3421 ctgctggtgg tgccgagctt cattgtgatc gcgatcgtca ctccgcgcct gggttcggcc
     3481 gaccgtgtct tctggttgat cctgttgttc tcggtgttcg gctggatgat cagctcgcgc
     3541 atcgtgcgcg gtatgacgat gagcctgcgg gaacgcgaat tcgtcgttgc ggcacggtac
     3601 atgggcgtg
//
LOCUS       DASYLX010000254         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_888587,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000254 DASYLX010000000
VERSION     DASYLX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888587"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VGP27_06305"
     CDS             <1..529
                     /locus_tag="VGP27_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7850961.1"
                     /translation="NAARLGAIAVGGVSIGAAVAVNWALAHPTRAIAVLAALPPWTGS
                     PETAPAALAARHSADVLRRDGLVSATSQMRASSPAWLADELTRSWLGQWPSLPDAMEA
                     ASRYASPTCAELERLTVPMGVAAATDDPVHPLEVGLEWVAAAPRAALHTVTLEEMGID
                     PSVLGTACVAALLDA"
     gene            complement(536..1318)
                     /locus_tag="VGP27_06310"
     CDS             complement(536..1318)
                     /locus_tag="VGP27_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEV7850962.1"
                     /translation="MMAFGKHAKPDDDGVGGDIVESDVESDVESDSAAGDDELDGPFD
                     IDDFDDPAAAAVARLDLGSVLIPMPEAGQVQVELTEGGVPSAVWVVTPNGRFTVAAYA
                     APKSSALWREVASELADGLRKDATEVRIEDGPWGREVVGAAPAGVVRFIGVDGYRWMI
                     RCVVNGPEQTIDELSDQARQALADTVVRRGETPLPVRTPLPVQLPEPMAAQLQAAAAE
                     QAAAQQAAQQQQQPPQTPQTQPVARRSEHGSAMQQLRTITGG"
     gene            complement(1315..1779)
                     /gene="dut"
                     /locus_tag="VGP27_06315"
     CDS             complement(1315..1779)
                     /gene="dut"
                     /locus_tag="VGP27_06315"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV7850963.1"
                     /translation="MSTSLAVVRLDRDLPMPSRAHDGDAGVDLFSAVDVELVPGHRAL
                     VPTGIAVAIPHGMVGLVHPRSGLAARVGLSIVNSPGTIDAGYRGEVKVALINLDPEQP
                     IVVHRGDRIAQLLVQRVELPELIEVTSFDEAGLTGTSRGDGGHGSSGGHASL"
     gene            1805..2296
                     /locus_tag="VGP27_06320"
     CDS             1805..2296
                     /locus_tag="VGP27_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEV7850964.1"
                     /translation="MSDTRAAPQVVRYSERLWVPWWWWPPGLALAALIALEVNQGVRA
                     LPNWVPFAVLLPVAAAVLLWFSKTEVRVISGGPDSPAHETELWVDDAHLPVSVVSRSA
                     EVPRSAKSAALGRQLDPAAYVVHRAWVGPLALLVLDDPDDPTPYWLVSCRHPDRVLSA
                     LRS"
     gene            complement(2303..2605)
                     /locus_tag="VGP27_06325"
     CDS             complement(2303..2605)
                     /locus_tag="VGP27_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HEV7850965.1"
                     /translation="MATDYDAPRRTETDDVSEDSLEELKARRNEAQSAVVDVDESDSA
                     ENFELPGADLSGEELSVRVIPKQADEFTCSSCFLVHHRSRLASEKNGMMICSDCAA"
     gene            2769..3425
                     /gene="cei"
                     /locus_tag="VGP27_06330"
     CDS             2769..3425
                     /gene="cei"
                     /locus_tag="VGP27_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope integrity protein Cei"
                     /protein_id="HEV7850966.1"
                     /translation="MVAQITEGTAFDKHGRPFRRRNFTPAIVVFAVLAVVTMIVWVIA
                     LNRAPDVTEAAACNPPADNTTSDPTQSKLGEQMTRASMTEVNPANLADTKIRVLNASG
                     QGGQAAEVAGQLRDLGFAEPTAENDPLYTNSRLACQGQIRFGPAGRAAAAAVWLVAPC
                     VELYQDQRPDSTVDLAIGTDFGELARSDDVEAVLSSLRPDATQPADPMLISKIHSAAC
                     "
     gene            complement(3458..4312)
                     /locus_tag="VGP27_06335"
     CDS             complement(3458..4312)
                     /locus_tag="VGP27_06335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7850967.1"
                     /translation="METLDLDPPELRSVAERLATEAADFVRRRREEVFSGTPQRESTG
                     QAVRAKSTPTDPVTIVDTETERLLRDRLAQLRPGEHVLGEEGGGISDDSAGRPTWVVD
                     PIDGTVNFVYGLPAYVVSVAVQVDGQSVAGAIANVVDGAVYSAARGHGAHVRLGETVA
                     RLRCSGVGDLSLSLVGTGFGYDPQLRRRQASVLVELLPQVRDIRRLGSAALDLCLVAA
                     GRLDAYFEDNLNVWDWAAGTLIAAEAGARVRVPSLSERAGGRGMVIAAAPGVNDAFVD
                     ALSRAAGL"
     gene            4395..>5178
                     /locus_tag="VGP27_06340"
     CDS             4395..>5178
                     /locus_tag="VGP27_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7850968.1"
                     /translation="MTATDSPAADPSTNGGQQRRGFGVDVGGSGVKGGIVDLDTGQLI
                     GERFKLATPQPATPDAVSKTVAAVVREFGWKDNVGVTYPGVVTDGIVRTAANVDKAWI
                     GCNATEFFGKELDGQQVTVLNDADAAGLAEEKFGAGRDNTGVIVLLTFGTGIGSAVIH
                     NGVLLPNTEFGHLEVGGKEAEHRAASSVKENKDWSYERWAKEVTRVLMAIENAIWPDL
                     FIAGGGISRKAEKWVPLLECRTPVVAAALQNTAGIVGAAMAAQ"
BASE COUNT          831 a         1794 c         1733 g          820 t
ORIGIN      
        1 caacgcggca cggctgggcg ccattgccgt cggcggggtt tccatcggag ccgcggtggc
       61 ggttaattgg gcgttggccc atccgacccg ggcgatcgcg gtgctggcgg cgctgccgcc
      121 ctggacgggc tcgccggaga ccgcgcccgc cgcgctcgcc gcccgacatt cggcggatgt
      181 ccttcggagg gacgggttgg tgtcggcgac gtcacagatg cgcgcgtcga gcccggcctg
      241 gctggccgac gagctgaccc ggtcctggct cgggcagtgg ccgtcgctac ccgacgcgat
      301 ggaggcggcg tcccggtacg cctcgcctac ctgcgccgag ttggagcggc tgacggtgcc
      361 gatgggcgtc gcggcggcca ccgacgaccc tgttcatccg ctggaggtcg gtctggagtg
      421 ggtggcggcc gcgccgaggg cggcgctgca caccgtcacg ctcgaggaaa tgggcattga
      481 cccgtcggtg ctcggcacgg cgtgcgtggc cgccctgctc gacgcgtgag ccttgctagc
      541 cgccggtgat ggtgcgcagc tgctgcatcg ccgacccgtg ctcactgcgg cgtgcgacgg
      601 gctgggtctg cggcgtctgc ggtggctgct gttgctgctg cgcggcctgc tgggcagcgg
      661 cctgctccgc ggcggcggcc tgcagctgtg cggccatcgg ctcgggcagc tgcaccggaa
      721 gcggggtgcg caccggcagc ggtgtctcac cgcgacggac aacggtatcg gctagggctt
      781 gtcgcgcctg gtcactcagt tcgtcgatgg tctgctccgg cccgttgacg acgcagcgga
      841 tcatccaccg gtagccgtcg acgccgatga accggaccac gccggcaggt gccgcgccaa
      901 cgacttccct gccccacggg ccgtcctcga tccgcacctc ggtggcgtcc ttgcgcagcc
      961 cgtccgccag ctccgatgcg acctcccgcc acagcgccga actcttcggc gcggcataag
     1021 ccgccacggt gaaccggccg ttgggcgtca ccacccacac cgcgctgggc acgccgccct
     1081 cggtcagttc cacctgaacc tggccggcct cgggcatcgg gatcagcacc gagcccagat
     1141 ccagccgggc caccgctgct gccgcggggt cgtcgaagtc atcgatgtcg aacgggccgt
     1201 ccaactcgtc gtcacctgca gccgaatccg attcgacatc cgattcgaca tccgattcga
     1261 cgatgtcgcc gccgacaccg tcgtcgtcgg gtttggcgtg ttttccaaaa gccatcacaa
     1321 actcgcatgt ccgccggagg aaccgtggcc gccgtcgcca cgggaggtgc cggtaagacc
     1381 ggcctcatcg aacgaggtca cctcgatcag ctcgggtaac tccacccgct gcaccagcag
     1441 ctgggcgatc cgatcaccac ggtgcacgac gatgggttgc tcggggtcga ggttgatcaa
     1501 cgccaccttg acctcgcccc gatagcccgc gtcgatcgtg ccggggctat tgacgatcga
     1561 aagtcctact cgcgcagcca aacccgagcg tggatgcacc aaccccacca tcccgtgcgg
     1621 aatggccacc gcgatccccg tcggcacgag ggcacgatga ccgggcacca attcgacgtc
     1681 gaccgcgctg aacaggtcga cgccggcgtc gccgtcatga gcccggctgg gcatcggcag
     1741 gtcgcggtcg aggcgaacga ctgccagaga ggtggacacg aggcatcaga ttacccttgg
     1801 cggcgtgtcc gacacgcgag cagcaccgca agtcgtgcgc tattcggaac ggctgtgggt
     1861 gccgtggtgg tggtggccgc ccggcctggc gctcgccgcc ctcattgcgc tcgaagtgaa
     1921 tcaaggcgtg cgcgccctgc ccaactgggt gcccttcgcc gtgctgctcc ccgtcgcggc
     1981 ggcagtgctg ttgtggttca gcaaaaccga ggtgcgggtg atcagcggcg ggccggacag
     2041 cccggcgcac gagaccgagc tgtgggtcga cgatgcgcat ctgcccgtca gcgtcgtctc
     2101 gcggtcagca gaagtgcccc ggtccgctaa gtcggccgcc ctggggcggc agctggaccc
     2161 ggccgcctat gtcgtgcatc gggcctgggt cggcccgctg gcgctactgg tgctcgacga
     2221 tccggacgat ccgacgccgt actggctggt cagctgtcgc catccagacc gggtgttgtc
     2281 ggcgctgcgc agttgacccg cctcaggccg cgcagtccga gcagatcatc atgccgttct
     2341 tctcgctggc cagccggctg cggtggtgca ccaaaaagca gctcgagcaa gtgaactcgt
     2401 cggcctgctt cggaatcacc cgcactgaca gctcttcgcc ggacagatcg gcgccaggca
     2461 gttcgaagtt ttcggcggaa tcagactcgt cgacgtccac gacggcagat tgggcctcgt
     2521 tgcgtcgagc cttgagttcc tcaagtgaat cctcagaaac atcgtcagtc tcggtacgcc
     2581 gtggcgcgtc gtagtcggta gccatctctt gtcccctccg tgaaccatgc gacagagctt
     2641 tgtaccagcg tcgaacgcat ccgccaaacg attcgtgccc ggattcccct atcgacaagt
     2701 gtgatttaca tcacaattgt gcgccgtgcc tgcccacggg gtgggagcgg gggttttaga
     2761 gtgcaggtgt ggtcgctcaa atcacagaag gcacggcctt cgacaagcac ggccggcctt
     2821 tccgccggcg caatttcacg ccggcgatcg tggtgttcgc ggtgctcgcc gtggtgacca
     2881 tgatcgtctg ggtgatcgcc ctcaatcgcg cgcccgatgt caccgaggcg gccgcgtgca
     2941 atccgcccgc cgacaacacc accagcgacc ccacccagtc caagctgggt gagcagatga
     3001 cacgcgcctc catgaccgag gtcaatcccg ccaacctggc cgacaccaag atcagggtgc
     3061 tcaacgccag cggacagggc ggccaggccg ccgaggtggc cgggcagcta cgagacctcg
     3121 gattcgccga gccgaccgcc gagaacgacc cgctctacac caacagccgg ttggcgtgcc
     3181 agggtcagat ccggttcggc cccgccggcc gcgcggcagc ggcggcggtg tggctcgtgg
     3241 ctccgtgcgt cgagctgtat caggaccagc ggcccgacag caccgtcgat ctcgcgatcg
     3301 gcaccgactt cggcgagctg gcgcgcagcg acgacgtcga agccgtgctg tccagtctgc
     3361 gaccggacgc cacccaaccg gccgacccga tgttgatctc gaagatccac agcgcagcct
     3421 gctgattgga ttaggctgca cccgttggtc ttcgtgacta cagcccggcc gcccgactca
     3481 gtgcgtcgac gaacgcgtcg ttgacgccgg gtgcggccgc aatcaccatg cccctgccac
     3541 cggcccgttc actcagcgat ggcacgcgca cccgcgcacc ggcctcggcg gctatcaacg
     3601 tccccgccgc ccagtcccac acgttgaggt tgtcctcgaa gtaggcgtcg agtcggccgg
     3661 ccgcgaccag gcacaggtcg agcgccgccg aaccgagccg acggatgtcg cgaacctgtg
     3721 gcagcagctc cacaagcacc gacgcctgac gtcgacgaag ttgtgggtcg tagccgaatc
     3781 cagtgcccac caaggacaat gacaggtcgc cgacaccgct gcagcgtagt cgcgccaccg
     3841 tttccccgag gcggacgtgg gcgccatgac cgcgcgcggc ggagtacacc gcgccgtcga
     3901 ccacgttggc gatcgccccg gccaccgact gcccgtcgac ctggacggcc accgagacca
     3961 cgtatgcggg caggccgtag acgaagttca ccgtgccgtc gatcgggtcc accacccagg
     4021 tcggacgacc ggcggagtcg tcggagatgc cgccaccttc ctcgccgagg acatgctcgc
     4081 cggggcgcag ctgcgcgagc cgatcgcgca gcagccgttc ggtttcggtg tcgacgatgg
     4141 tgacggggtc ggtcggcgtg ctcttggcgc gcaccgcctg cccggtcgac tcccgctgcg
     4201 gtgtgccgct gaagacttcc tcgcgacggc gacggacgaa gtccgccgcc tcggtcgcca
     4261 acctttcggc gaccgagcgg agttcgggcg ggtcgaggtc gagtgtttcc accggcttat
     4321 cgcagcacaa attgtcccca accggttcat cagcacccct accaaagcat tgccgagcca
     4381 ctaatgttgg gctgatgacc gccaccgatt cacctgccgc cgatccgagc accaacggag
     4441 gccagcaacg ccgcggtttc ggtgtcgacg tgggcggtag cggcgtcaag ggcggcatcg
     4501 tcgacctcga caccggtcag ctgatcggcg agcgcttcaa gctcgcgacc ccgcagcccg
     4561 ccacccccga cgcggtcagc aagaccgtgg cagccgttgt tcgcgagttc ggctggaagg
     4621 acaacgtcgg cgtcacctat cccggcgtgg tcaccgacgg catcgtgcgc accgcggcca
     4681 acgtcgacaa ggcgtggatc ggatgcaatg ccaccgagtt cttcggcaag gaactcgacg
     4741 gccaacaggt gaccgtgctc aacgacgccg acgccgccgg gctcgccgag gagaagttcg
     4801 gcgcaggccg cgacaacacg ggcgtcatcg tgctgctgac gttcggcacc ggcattggct
     4861 cggcagtgat ccataacggt gtgctgctgc ccaacacgga gttcggccac ctcgaggtcg
     4921 ggggtaaaga ggccgagcac cgggccgcct cgtcggtcaa ggagaacaag gactggagct
     4981 atgagcgctg ggccaaagag gtcaccagag tgctgatggc gatcgagaac gcgatctggc
     5041 ccgacctgtt catcgcgggc ggtggcatca gtcgcaaagc cgagaaatgg gtcccgctgt
     5101 tggagtgccg gacgccggtg gttgccgcgg cgctgcagaa cacggcggga atcgtcggcg
     5161 cggcgatggc cgcccagg
//
LOCUS       DASYLX010000255         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2752631,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000255 DASYLX010000000
VERSION     DASYLX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752631"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VGP27_06345"
     CDS             complement(<1..1020)
                     /locus_tag="VGP27_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7850969.1"
                     /translation="MSPANAGYLAPAVTLSSSLPKRKADKTVLIVPVVSGGDDDAKAT
                     VVANPFLDAEAVGEIEAALDSLGAKGGSDQVTRVVAPSLPVASVLAVGLGKARDAWPS
                     DLIRRAAGVAARSLNGTEAVITTLSDIDLEAAVEGLVLGAYRFSDFRSDKTAPKDKGL
                     RDITALSVDTKGKTTAARTRGAPKAQAQRATNIATAVATARDLVNTPPSHLYPDEFAK
                     RAQAFGEAAGLEVEVLDDKALAKAGYGGIIGVGKGSSRPPRLVRLAHRGAKRKGKKVA
                     LVGKGITFDTGGISIKPAANMHHMTSDMGGAAAVIATVVLAAKQNLPIDVIATVPMAE
                     NMPSAT"
     gene            complement(1039..>1903)
                     /locus_tag="VGP27_06350"
     CDS             complement(1039..>1903)
                     /locus_tag="VGP27_06350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850970.1"
                     /translation="DIATAWAVLGLTVASPDEVALSQADVDGLATWVAMKNVMTDDGA
                     FGFLRVLGAAMARLAEAESAVVRARAPDLQMNHSHDELVTAQAYRQVAGFIPRIGALI
                     DAVHRHHLISARTHFEGVIRDTSETVVCGIGFADLSGFTALTQQLTPAELSNLLNEFS
                     ATVTDVVHADGGRVVKFIGDEVMWVSSTPERLAKAAADLVDHPKAREAQMQVRAGLDY
                     GTVLAVGGDYFGNSVNLAARLVAAAEPGQILATSALRDELPNWPAIPQEPLTLKGFDT
                     PIAAFDLHVPG"
BASE COUNT          279 a          628 c          655 g          341 t
ORIGIN      
        1 ggtggccgac ggcatgttct cggccatcgg gacggtggcg atcacgtcga tgggcaggtt
       61 ctgcttggct gcgaggacca ccgtcgctat cacggccgcg gcaccaccca tgtcggacgt
      121 catgtggtgc atgttggcgg ccggcttgat cgagatgccg ccggtgtcga acgtgatgcc
      181 cttgcccacc aacgcaacct tcttgccttt tcttttcgcg cctcggtgtg ccagccggac
      241 aaggcgcggc ggccgcgagg accccttgcc gacgccgatg atgccgccgt aacccgcctt
      301 ggcgagtgcc ttgtcgtcga gtacctcgac ctcgagacct gcggcttcgc cgaaagcctg
      361 tgcgcgcttg gcgaattcgt ccgggtatag gtgcgacggt ggtgtgttga ccaggtcgcg
      421 cgcggtggcg acggccgtcg cgatgttcgt ggcgcgctgc gcttgggcct tcggtgctcc
      481 tcgcgtgcgc gcagccgtgg ttttgccctt cgtgtccacg ctcagcgcgg tgatgtcgcg
      541 caggcccttg tccttcggcg cggttttgtc gctgcggaag tcgctgaacc ggtaggcgcc
      601 gaggaccagg ccctcgacgg ccgcctccag gtcgatatcc gacagcgtgg tgatcacggc
      661 ctcggtgccg ttcagtgacc gggccgccac gcccgccgcg cgccggatca ggtctgaggg
      721 ccaggcgtcg cgggccttgc cgaggccgac cgcgagcacg ctggccaccg gcagcgacgg
      781 cgcgaccacc cgcgtgacct ggtcgctgcc gcctttggcg ccgagtgagt cgagcgcggc
      841 ctcgatctcg ccgacggcct cggcgtcgag gaacgggttg gcgacgacgg tcgctttcgc
      901 gtcgtcgtct ccgccgctga ccaccggcac gatcagcacc gtcttatcgg ccttgcgttt
      961 gggcagcgac gagctgaggg tgacggctgg ggccagataa ccggcatttg cggggctcac
     1021 gggtattgag cctatcggtc agcccggcac gtgcagatcg aaggccgcga taggggtatc
     1081 gaagcctttg agcgtcaacg gttcctgagg aatggcgggc cagttgggca gctcgtcacg
     1141 cagggccgac gtggcaagga tctgcccggg ctcggcagcc gcgaccagcc gggcggccag
     1201 gttgaccgag ttgccgaagt agtcgccccc gacggcgagg accgtgccgt agtcgagacc
     1261 ggcgcggacc tgcatctgcg cctcgcgcgc cttcgggtga tcgaccagat ccgccgcggc
     1321 cttggccaat cgttcgggtg tggaactcac ccacatcacc tcgtcgccga tgaacttgac
     1381 cactcgaccg ccgtcggcgt gcaccacatc ggtcacggtg gcgctgaatt cgttgagcaa
     1441 gttcgacaac tccgcgggtg tgagctgctg tgtcaaagcg gtgaagcccg acagatccgc
     1501 gaaaccgata ccgcagacaa ccgtctccga cgtgtcgcga atgacgccct cgaagtgcgt
     1561 cctggcgctg atcaagtggt ggcggtgaac cgcatcgatc agtgctccga tgcgggggat
     1621 gaaccccgcc acctgtcggt acgcctgtgc ggtgacgagt tcgtcatgac tgtggttcat
     1681 ttgcagatcc ggcgcgcggg cgcggacaac ggccgactcg gcttccgcca ggcgcgccat
     1741 cgccgcgccg aggacacgta ggaagccgaa cgcgccatca tcggtcatta cgttcttcat
     1801 cgccacccag gtcgccaggc catcgacatc ggcttggctc aaggcgacct cgtcggggct
     1861 cgcgacggtg aggccgagca ccgcccacgc ggttgcgatg tcg
//
LOCUS       DASYLX010000256         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3741189,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000256 DASYLX010000000
VERSION     DASYLX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3741189"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1726
                     /locus_tag="VGP27_06355"
     CDS             83..1726
                     /locus_tag="VGP27_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV7850971.1"
                     /translation="MYQQVLDPVGHSLAWSSLVAAIPLLLLFVLLGVLRVTAWVASLI
                     SLAVAIAIAVLVYGMPIGQSLLAGTEGAAFGFFPILWIVINAIWVYQQTVETGHFDVL
                     RRSFSRVSDDQRIQAVLIAFSFGALLEALAGFGTPVAVTSVMLMALGFKPMKAAVLAL
                     VANTAPVAFGAMATPILTLAKVTELPSDTLGAMVGRQTPILAVFVPLVLVAIVDGWRG
                     IRETWPAAVVCGLVFGVAQYATSNFISVPLADVVASLLSAAAVVLLVRVWRPARPYTE
                     PAVVAGGAADEPTPEFAQRVEHPDSRSDVVKAYAPYAIIIAVFVICQITAVKKLLDKA
                     TVKFQWPGLDIVSPKGKPVALTEFTLNLLTTPGTQMLIAGILTAIVLKLSVPRAVKAY
                     GTTLHQLRWAIVTVMAVLALAFVMNLSGQTITLGTWMAAAGGAFALLSPILGWLGVAV
                     TGSDTSANSLFGALQVTAANQAGLSDVLMAASNSSGGVLGKMVSPQNLAIAAAAVGMN
                     GKEGDIFRRVVVWSLLFLLLMCILSALQASPVLSWMVPS"
     gene            1788..3107
                     /locus_tag="VGP27_06360"
     CDS             1788..3107
                     /locus_tag="VGP27_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7850972.1"
                     /translation="MVVTDPAITEGYRQDRALDPAAGKPLAVVRPLTTEHVQIIMRWA
                     SAHRVPVVPRGAGSGLSGGATALDDGIVLSTEKMRDITVDPVTRTAVCQPGLFNAEVK
                     KAVAQHDLWYPPDPSSFEICSIGGNIATNAGGLCCVKYGVTTDYVMGLQVVLADGTAV
                     RVGGPRLKDVAGLSLTKLFVGSEGTLGIVTEITLRLLPKQHRSATVVANFATVDAATD
                     AILGVACQMRPSMLEFMDAVAINAVEDKLKMGLDRDAAALLLAGSDERGRSAAEDAKM
                     IADVFGESGATDVFTTDDPDEGEAFVAARRFAIPAVEAMGTLLLEDVGVPMPRIGDLV
                     RGVERIATEGDVMISVIAHAGDGNTHPLIVYDPADAAMAERAERAFGQIMDLAIGLGG
                     TITGEHGVGRLKRPWLEDQVGPEVMDLNRRIKQALDPGNILNPGAAI"
     gene            3164..>3483
                     /locus_tag="VGP27_06365"
     CDS             3164..>3483
                     /locus_tag="VGP27_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7850973.1"
                     /translation="MTATLSHRTPARFELATAATWPNPWPMYQALRDKDPVHHVVPED
                     RPDLDYWVVSRHADVWNAARDHETFSSAQGLTVNYGELEMIGLADNPPFVMQDPPTHT
                     EFRKL"
BASE COUNT          554 a         1154 c         1162 g          613 t
ORIGIN      
        1 cagtagaacg ctttcgttaa gagcccactg atcttgggta cccgtgtgac gcaaaccaca
       61 aatcgaacat cgaggttggc tcgtgtacca gcaagttctc gatccggttg gccattccct
      121 cgcgtggagt tcgctggtcg cggcgatccc cctgctgctg ttgttcgtgc tgctcggcgt
      181 cctgagagtg accgcatggg tggcgtcgct gatatcgctt gccgttgcca tcgccatcgc
      241 tgtcctggtc tacgggatgc cgatcggtca gtcgctgctg gcaggcaccg aaggtgccgc
      301 gttcggcttc tttccaatct tgtggatcgt catcaacgcg atctgggtct accagcagac
      361 cgttgagacc gggcacttcg acgtgctgcg ccgctcattc agccgagtga gcgacgacca
      421 gcgcatccag gccgtgctga tcgcgttctc gttcggcgcg ctgctcgaag cgctggccgg
      481 gttcggcaca ccggtggcgg tgacgtcggt gatgctgatg gcgttgggct tcaagccgat
      541 gaaggcggcg gtgctcgccc tcgtcgcgaa caccgcgccg gttgccttcg gcgcgatggc
      601 gacaccgatc ctcaccctcg ccaaggtgac cgagttgccc agcgacacac tcggcgcgat
      661 ggtgggtcgt cagacgccga ttctggcggt gttcgtgcct cttgtcctcg tcgcgatcgt
      721 cgacggctgg cgcggcatcc gcgagacttg gcctgcggcc gtcgtgtgcg ggctcgtctt
      781 cggggtcgcc cagtacgcca cctccaactt catctcggtg ccactggcgg acgtcgtcgc
      841 gtcgctgctg tcggccgccg cggtcgtgct gctggtgcgt gtctggcggc cagcaaggcc
      901 ctacacagag cccgccgtcg tcgcgggcgg tgcggccgac gagccgacac ccgaattcgc
      961 ccaacgtgtc gagcatcccg actcgcggtc cgatgtcgtc aaggcctatg ccccgtacgc
     1021 gatcatcatc gcggtcttcg tcatctgcca gatcaccgcc gtcaagaagc ttctcgacaa
     1081 ggcgacggtc aagttccagt ggcccggcct ggacatcgtg agtcccaagg gaaagccggt
     1141 ggcgctcacc gaattcaccc tgaacctgct caccacgccg ggaacgcaga tgctgatcgc
     1201 gggcatcctg acggcgatcg tgctcaagct gtcggtaccg cgcgccgtca aggcgtacgg
     1261 cacaacactg catcaactgc gctgggcgat cgtcacggta atggcggtgc tggcgctggc
     1321 gttcgtaatg aacctgtccg gccagacgat cacgttgggc acctggatgg ccgcggccgg
     1381 cggtgcgttc gcgctgctgt cgccgatcct cggctggctc ggtgtcgcgg tcaccggatc
     1441 cgacacctcg gccaactcac tgttcggcgc gcttcaggtc accgcggcca accaggccgg
     1501 gctgtccgat gtgctgatgg cggcgtccaa cagttccggc ggtgtgctgg gcaagatggt
     1561 ctcgccgcag aacctcgcca tcgcggccgc agcggtcggt atgaacggca aggaaggcga
     1621 catcttccgc cgagtcgtgg tgtggagcct gctgttcctg ttgctcatgt gcatcctgtc
     1681 cgcgctgcag gcgtcgccgg tgctgtcctg gatggtgccg tcgtgatgac tcgtagcatc
     1741 gaaccgtgag cgttctcgac agcctgaccg ccgacctgcc cgacggcatg gtcgtcaccg
     1801 accccgcgat caccgagggg taccgccagg accgcgcgct cgacccggcg gcgggcaagc
     1861 cgctggccgt cgtgcgcccg ctgaccaccg agcatgtgca gatcatcatg cgctgggcga
     1921 gcgcccaccg ggttccagtc gtgccgcgcg gggcgggcag cggactctcc ggcggggcca
     1981 ccgccctcga cgacggcatc gtgctgtcca ccgagaagat gcgcgacatc accgtcgacc
     2041 ccgtcacgcg caccgcggtc tgtcagccag gcctgttcaa cgccgaggtg aaaaaggccg
     2101 tcgcgcaaca tgatctgtgg tacccgccgg acccgtcgtc gttcgagatt tgcagcatcg
     2161 gcggcaacat cgccaccaac gcgggcgggc tgtgctgcgt caagtacgga gtcaccaccg
     2221 actatgtgat gggtttgcag gtggtgctgg ccgacgggac ggcggtacgg gtcggcggac
     2281 ctcggctgaa ggatgtcgcc gggctcagcc tcacgaagct gttcgtcggc agcgagggca
     2341 cgctgggcat cgtcaccgag atcacgctgc ggctgctgcc caagcagcac cggtcggcca
     2401 ccgtggtggc aaatttcgcg acggtcgacg cggccaccga cgcaatactg ggggtggcct
     2461 gccagatgag gccgtcgatg ctcgagttca tggacgccgt ggccatcaac gccgtcgagg
     2521 acaagttgaa gatgggcctg gaccgggacg cggccgcgct gttgctggcg ggctccgacg
     2581 aacggggccg gtcggcggcc gaggacgcga agatgatcgc cgacgtgttc ggtgagagtg
     2641 gcgcaaccga tgtgttcacc accgacgacc ccgacgaggg cgaggcgttc gtcgccgccc
     2701 gccggttcgc gattccggcg gtggaggcca tgggcacgct gctgctcgaa gacgtcggcg
     2761 tgccgatgcc gcgcatcggc gacctcgtcc gcggcgtcga gcgcatcgcc accgagggcg
     2821 acgtgatgat ctcggtgatc gcacacgcag gcgacggcaa cacccatccg ttgatcgtgt
     2881 acgaccccgc cgacgccgcg atggcggaac gggccgagcg cgcgttcggc cagatcatgg
     2941 atctcgcgat cggactcggc ggcaccatca ctggcgagca cggcgtcggc agactgaaaa
     3001 ggccctggct ggaggaccag gtcgggcccg aggtgatgga cctcaaccgg cgcatcaaac
     3061 aggcgcttga tcccgggaac atcctgaatc cgggtgccgc catttgatga caattcggtg
     3121 cgccctgttg acagggcatg cgttcctgtc gtactcataa gacatgaccg ccacactgtc
     3181 tcaccgcacg cctgcccggt tcgaactcgc caccgccgcg acttggccca acccgtggcc
     3241 gatgtaccag gcactgcgcg acaaggaccc ggtgcaccac gtggtcccgg aagaccggcc
     3301 cgatctcgac tactgggtgg tgtcgcggca cgcggacgta tggaacgcgg cgcgcgacca
     3361 cgagacgttt tcgtcggcgc agggcctcac ggtcaactac ggcgaactcg agatgatcgg
     3421 tctcgccgac aacccgccgt tcgtgatgca ggatccgccg acgcacaccg agttccgcaa
     3481 gct
//
LOCUS       DASYLX010000257         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_559466,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000257 DASYLX010000000
VERSION     DASYLX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559466"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..412
                     /locus_tag="VGP27_06370"
     CDS             2..412
                     /locus_tag="VGP27_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850974.1"
                     /translation="MFASCVFVYITRLERRTSHPLGEKVGAHKTVLAKLRKREPMSQD
                     EVDYAGELIADARSPLAYAIPAALFTIGFFYVVGCLYMLHRDGGNPSFRTFIGGIPML
                     TSMNMAAQLRRVAGMKGKLRDVSAMAPEPAYPGR"
     gene            complement(473..>3194)
                     /locus_tag="VGP27_06375"
     CDS             complement(473..>3194)
                     /locus_tag="VGP27_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7850975.1"
                     /translation="RIGKVFGEFDSDSSAMIVLEGDQPLGADAHHYYDGLIEKLRKDT
                     KHVEHIQDFWGDPLTAAGSQSNDGKAALVQLYLAGNQGESLANESVDSVRDIVNNTPP
                     PPGLKAYVTGAAPLVTDQFEVGRHGTLKTTLITLSVIAVMLFSLYRRLTTVFLVIFTV
                     MIELTASRGVVAVLANAGIIQLSTYSTNLLTLLVIAAGTDYAIFILGRFHEARYDGQD
                     RVSAFNTMYHGTAHIILGSGLTIAGAVLCLTFTRLPYFNSLGVPAGIGVLVAVVAALT
                     LAPALLIIGRHFGLFEPSRRMQTRGWRRIGTAIVRWPVPILLVTVAIATIGLLSLSGY
                     KTSYDARPFMPASAPANVGYAAAERHFSEARLNPELLMVEADHDLRNSTDMILLERVA
                     KAVFHTDGIAQVQSITRPLGTPLDHTSIPFQISAGSAAQINNLPFQQARGADLLKQVD
                     VINNSIDVLRQQYALQQQSGAATDEQTKAFQQTVAVANDLRDKIANFDDFFRPLRNYF
                     YWEPHCFDIPACAALRSLFDALDGIDALTDQLASVSGSINKLDQLQPKLLALIPPQIA
                     SQQTNRELTLTNYATTSGIDDQTEAALQNSTALGQAFDASKTDDSFYLPPEAFTNPEF
                     LRGMKLFMSPDGKAARMIITHEGIPATPEGISHIDPMRHAAQEAVKGTPLAGSKIFIA
                     GTAATYKDIQDGAKYDLMIAGIAALSLILLVMMFITRSIVAAIVIVGTVALSLGASFG
                     LSVLIWEDIFGIPLYWIVLALAVILLLAVGSDYNLLLISRFKEEIGAGINTGIIRAMA
                     GSGAVVTAAGLVFAATMASFVFADLRILGQIGTTIALGLLFDTLIVRSFMTPSVAALL
                     GRWFWWPLRVRTRPAGRGLQPSESGRSGRQLRLWDDGDSAVSDAPPRSQA"
BASE COUNT          619 a          947 c         1046 g          582 t
ORIGIN      
        1 tgtgttcgcc tcgtgcgtat tcgtctacat cacccggctt gagcggcgga catcccatcc
       61 attaggtgag aaagtcggcg ctcacaagac tgtgctggcc aaactccgca agcgcgagcc
      121 gatgtcgcag gacgaagtgg actatgcggg tgaactgatc gctgatgctc gatcaccgct
      181 cgcgtacgcg atacctgccg ccctcttcac catcggattc ttctatgtgg tcggctgtct
      241 ctacatgctg caccgagatg gaggcaatcc ctcctttcga acgttcatcg gcggtatccc
      301 gatgttgacc tccatgaaca tggccgctca actgcgcagg gtcgcgggga tgaaggggaa
      361 acttcgggac gtttcggcga tggcgcccga gcccgcatac cccggccggt agcacctgac
      421 ccgaggcgat cagcgagagt aactgtcgcg caagaggatc gaatctcgat cgtcatgctt
      481 gcgaacgtgg cggtgcgtcg gagaccgctg aatcgccgtc gtcccacagc cgtagttgac
      541 gacctgatcg acctgattcg gatggctgaa gccctcggcc ggcggggcgg gtgcgcaccc
      601 gtagcggcca ccagaaccag cgcccgagca gtgccgcgac cgacggcgtc atgaatgaac
      661 gcacgatcag cgtgtcgaac agcagaccga gggcgatcgt cgttccgatc tgaccaagaa
      721 tccgcaagtc ggcgaacacg aaggaggcca tggtcgccgc gaacaccaga ccggcggcgg
      781 tcaccaccgc gcccgaaccc gccatcgccc ggatgatgcc cgtgtttatg ccggcgccga
      841 tctcctcctt gaaccgggag atcagcagca ggttgtagtc cgaccccacc gccaagagca
      901 ggatcaccgc cagcgccagc acgatccagt acagcgggat accgaaaatg tcctcccaaa
      961 tcagcacgga aagcccgaac gaggcaccca acgacaacgc cacggtgccc acgatgacga
     1021 tcgcggcgac gatgcttctg gtgatgaaca tcatcacgag cagaatcagg ctgagcgcag
     1081 cgatcccggc gatcatcagg tcgtacttgg cgccgtcctg aatgtccttg taggtggccg
     1141 cggtaccggc gatgaagatc ttcgacccgg ccaggggcgt gcccttgacg gcctcctgcg
     1201 cggcgtgccg cattgggtcg atgtgggaaa tgccttcggg ggttgcggga atgccttcgt
     1261 gggtgatgat catgcgggcg gccttgccgt ccggtgacat gaacagcttc atcccgcgca
     1321 gaaattctgg gttggtgaac gcctcgggcg gcagatagaa cgagtcgtcg gtcttcgacg
     1381 cgtcgaaagc ctggcccaaa gcggtggagt tctgcagtgc cgcctcagtc tggtcgtcga
     1441 tgcccgacgt ggtggcgtaa ttcgtcagcg tgagttcgcg gttggtctgc tggctcgcga
     1501 tctgaggcgg aatcagcgcg agcagcttcg gctgcagctg gtccagcttg ttgatgctcc
     1561 ccgagacgct ggcaagctgg tcggtcagcg cgtcgatccc gtcgagtgcg tcgaacagcg
     1621 atcgcagcgc ggcgcaggcg gggatgtcga aacagtgtgg ctcccaatag aagtagttgc
     1681 gcaagggccg aaagaagtca tcgaagttgg cgatcttgtc gcgcagatcg ttggccactg
     1741 cgaccgtctg ctggaatgcc ttggtctgct cgtcggtggc ggcgccggac tgttgttgca
     1801 gcgcgtactg ctgccgcagc acatcgatcg agttgttgat cacgtcgacc tgcttgagca
     1861 ggtcggcgcc gcgggcctgt tggaagggca ggttgttgat ctgagccgcg ctgcccgcac
     1921 tgatctggaa cggtatcgac gtgtggtcca agggtgtgcc cagcggccgg gtgatcgact
     1981 gcacctgagc gatgccgtcg gtgtgaaaaa ccgccttcgc aacgcgctcg agaaggatca
     2041 tgtcagtcga atttcgcaga tcgtggtcgg cttcgaccat caacagttcg ggattgagcc
     2101 gtgcctccga aaaatggcgt tcggcggccg catagccgac attcgccggc gcactggcgg
     2161 gcatgaacgg acgggcgtcg tagctcgtct tgtatcccga cagggagagc agaccgatgg
     2221 tggcaatggc gaccgtcacc agcaggatgg ggacgggcca ccggacgatt gctgtgccaa
     2281 tgcgtcgcca gccccgggtt tgcatgcgtc gactgggttc gaacagaccg aaatgtcggc
     2341 cgatgatcag caaggccggc gccagggtca acgccgcaac caccgcgacg aggacgccga
     2401 taccggcggg aacgccaagg ctgttgaaat aggggagccg ggtgaaggtc agacagagca
     2461 ccgcccctgc gatggttagg cccgaaccca ggatgatgtg tgcggttccg tggtacatcg
     2521 tgttgaaagc cgagacgcga tcctgtccgt cgtaacgcgc ttcgtggaaa cggccgagaa
     2581 tgaagatcgc atagtcggtt ccggcggcga tgaccagtag cgtcagcagg ttggtcgagt
     2641 acgtcgacag ctggatgatg ccggcgttcg ccagcacggc gacaacgccg cgcgacgcgg
     2701 tcaactcgat catcaccgtg aaaatcacga gaaacaccgt ggtgaggcga cggtagagcg
     2761 agaacagcat caccgcgatc accgagaggg tgatcaaggt cgtcttcaaa gtgccgtggc
     2821 ggcccacttc gaactggtcg gtgaccaggg gcgcagcgcc ggtgacatac gccttgaggc
     2881 ccggcggggg tggtgtgttg ttgacgatgt cacgcaccga atcaacggac tcattggcta
     2941 acgactcgcc ctggttgccg gcgaggtaca gctgcaccaa cgccgccttg ccgtcgttac
     3001 tctgcgagcc tgccgccgtc agcgggtcac cccagaagtc ctgaatgtgc tcgacgtgct
     3061 tggtgtcctt gcggagcttt tcgatcaagc cgtcgtagta gtggtgggcg tcggcaccga
     3121 gcggctgatc tccctccagg acgatcatcg ccgaactgtc ggagtcgaac tcgccgaaca
     3181 ccttgccgat gcgt
//
LOCUS       DASYLX010000258         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_779113,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000258 DASYLX010000000
VERSION     DASYLX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779113"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..688)
                     /locus_tag="VGP27_06380"
     CDS             complement(59..688)
                     /locus_tag="VGP27_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7850976.1"
                     /translation="MNGGPIQRVVTHGTFELDGGSWEVDNNVWVVGDENDVVVFDAAH
                     DAGPIVNAVGGRNVVAVVCTHGHNDHVTVAPELGDALDAPVLLHPADDVLWRMTHPDK
                     DFRAVGDNETLRVAGTELHALHTPGHSPGSVCWYAPELGAVFSGDTLFEGGPGATGRS
                     YSDFPTILESIAGRLGKLPGDTIVYTGHGDSTTVGDEIVHYDEWVARGH"
     gene            complement(685..1773)
                     /locus_tag="VGP27_06385"
     CDS             complement(685..1773)
                     /locus_tag="VGP27_06385"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEV7850977.1"
                     /translation="MGQTVRGVISRKKGDPVEVVDVVIPDPGPGEVVVDIQACGVCHT
                     DLTYREGGINDEYPFLLGHEAAGIVDSVGEGVTNVEPGDFVILNWRAVCGQCRACKRG
                     RPHLCFDTFNASQKMTLTDGTELTPALGIGAFVDKTLVHEGQCTKVDPAADPAVAGLL
                     GCGVMAGIGAAINTGAVNRDDTVAVIGCGGVGDAAIAGARLVGAKRIIAVDTDNKKLD
                     WARGFGATHTINAKELDPVATIQDLTDGNGADVVIDAVGRPETWKQAFYARDLAGTVV
                     LVGVPTPDMKLEMPLVDFFARGGALKSSWYGDCLPERDFPTLISLYLQGRLPLEKFVS
                     ERIGLDDIEDAFHKMHAGEVLRSVVVFK"
     gene            1772..>1951
                     /locus_tag="VGP27_06390"
     CDS             1772..>1951
                     /locus_tag="VGP27_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850978.1"
                     /translation="MSQTLAGHVGEPTVVHVGERTVVHGHGPIVERIGGPMMETCTHD
                     AGPLFEPRCPAARRRR"
BASE COUNT          328 a          688 c          611 g          324 t
ORIGIN      
        1 tcaatctcca agcatggtca gagcaatggg ccgccacgtc agggcgcaag acggaagcct
       61 agtggccccg ggccacccat tcgtcgtagt gcacgatctc gtcgccgacg gtggtggagt
      121 cgccgtgtcc ggtgtagacg atggtgtcgc ccggcaactt gccgagccgg cccgcgatgg
      181 actccaaaat cgtcgggaag tcggagtacg accggcccgt cgcgccaggt cctccttcga
      241 acagcgtgtc gccgctgaac accgcaccca gctccggggc ataccagcac accgaccccg
      301 gcgaatgccc cggcgtgtga agagcatgca gctcggtgcc tgccacccgt aacgtctcgt
      361 tgtcgccgac ggcgcggaag tccttgtccg ggtgcgtcat ccgccacagc acgtcgtcgg
      421 cggggtgcaa cagcacagga gcgtccagcg cgtccccgag ttcgggcgcc accgtcacgt
      481 ggtcgttgtg gccgtgtgtg cagacgaccg cgaccacgtt gcgcccgccg acggcgttga
      541 cgatggggcc tgcgtcgtgc gcggcatcga agaccaccac gtcgttctcg tcgccgacga
      601 cccagacgtt gttgtcgact tcccagctgc cgccgtcgag ttcgaacgtg ccatgggtga
      661 cgacgcgctg tatcgggccg ccgttcactt gaacaccacc accgagcgca gcacctcacc
      721 ggcatgcatc ttgtggaagg cgtcctcgat gtcgtcgagg ccgatgcgct cggagacgaa
      781 cttctccaac gggagccggc cctgcaaata caggctgatc agggtgggga agtcgcgctc
      841 gggcaggcag tcgccgtacc acgacgactt cagcgcgccg ccgcgggcga agaagtcgac
      901 cagcggcatc tccagcttca tgtccggagt cggaacccca accagtacaa cggttcccgc
      961 cagatcgcgg gcatagaacg cctgcttcca ggtctccggc ctgccgaccg catcgatcac
     1021 cacgtcggcg ccgttgccgt cggtcaggtc ctgaatggtc gccaccgggt ccagctcttt
     1081 ggcgttgatg gtgtgggtgg cgccgaagcc tcgcgcccag tccagcttct tgttgtcggt
     1141 gtccacggcg atgattcgct tagccccgac cagccgggca ccggcgatcg ccgcgtcgcc
     1201 cactccgccg caaccgatca cggcgacggt gtcgtcccgg ttcaccgcgc cggtgttgat
     1261 cgccgcaccg atgccggcca tcacaccgca gcccagcagc ccggccacag cggggtcggc
     1321 cgctggatcg accttcgtgc actgcccctc gtgcaccagc gtcttgtcca cgaacgcgcc
     1381 gatacccagc gccggagtca gttccgtgcc gtcggtcaac gtcatcttct gcgaggcatt
     1441 gaaggtgtcg aagcacagat gcggacggcc gcgcttgcag gcgcggcact gcccgcacac
     1501 cgcgcgccag ttcaggatca cgaagtcccc tggctccacg ttcgtcacgc cctcgccgac
     1561 cgagtcgacg atgcccgcgg cctcgtggcc cagcaggaac ggatactcgt cgttgatgcc
     1621 gccctcgcga taggtcaggt cggtatggca gacaccgcac gcctggatgt cgaccaccac
     1681 ctcacccggg ccgggatcgg ggatgacgac atccaccacc tcgaccgggt cacccttctt
     1741 cctcgatatc actccgcgca ctgtttgacc catgagtcaa accttagcgg gacacgtcgg
     1801 cgagccgaca gtggtgcacg tcggcgagcg gacagtggtg cacggtcatg ggccgatagt
     1861 ggaacgcatc ggcggcccga tgatggaaac ctgtacccat gacgccggtc ctctatttga
     1921 gccacggtgc cccgccgctc gtcgacgacg a
//
LOCUS       DASYLX010000259         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2204582,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000259 DASYLX010000000
VERSION     DASYLX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204582"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGP27_06395"
     CDS             <1..685
                     /locus_tag="VGP27_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7850979.1"
                     /translation="NVLRDVGLGYQPFGGGGREGYVRYENVRVPNDHMLGPRGGAFVV
                     AQTRLGGGRIHHAMRTVGLVGRIFDMICERAVSRYTQGDLLSNKQLVQEMVADSWMEI
                     EAFRLLTLQTAWKIDQHNDYKAVRADISAVKAMMQKVLHHVSARALQLHGSLGTTHEM
                     PFVQYLVESFVLGLADGPTEVHKVTLARLLLKDYKPAPDVFPSQHLLRLREAAEKKFA
                     DKLAGVPRT"
     gene            700..1482
                     /locus_tag="VGP27_06400"
     CDS             700..1482
                     /locus_tag="VGP27_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7850980.1"
                     /translation="MPCDGKVALVTGTSRGLGKAIAQRLAAEGATVALTARTMEPDPK
                     YKGSLRQTLDEIEAAGGSGVAIQADLSKAEDRERLFGEVVERVGAPDILVNNAAVTFL
                     RPLDEFPERRVRLMMEMHVLAPLHLTQLAIPAMRHRGRGWVLNVTSVGGDLPEGPPFS
                     EFDRVAGFGVYGTVKAALNRLTKSLAAELYGDGIAVNAAAPTNPVATEGAGALDLAKT
                     DTEGIELITETAFLLCTGNPTTLTGRIAHTQEFLREVGWSST"
     gene            1512..>2087
                     /locus_tag="VGP27_06405"
     CDS             1512..>2087
                     /locus_tag="VGP27_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7850981.1"
                     /translation="MSTEAVTAARVLSSDRSTATREALLLAAERLFAERGMYAVSNRQ
                     ISEAANQGNNAAACYHFGTRTDLLRAIESKHRAPIDKLRGRMLADVAGSTELRDWVGT
                     LVLPLTDHLTALGSPSWYARFAAQAMADPSYRQVVTKDALTSPELVAAINGINDCLPD
                     LPKRVRIERIVMARNLLMHTCAEHEGELAEHG"
BASE COUNT          382 a          695 c          703 g          307 t
ORIGIN      
        1 caacgtgctg cgcgacgtcg gcctcggcta tcagccgttc ggtggcggcg gccgggaagg
       61 ttacgtccga tacgagaacg tccgcgtccc caacgatcac atgctcggac cgcgcggcgg
      121 cgcgttcgtc gtcgcccaga cacgactcgg cggcggacgc attcaccacg caatgcgcac
      181 ggtcgggctg gtcggacgca tcttcgacat gatctgcgag cgcgccgtgt cgcgatacac
      241 ccagggcgac ctgctgtcca acaagcagct cgtgcaggaa atggtcgccg actcatggat
      301 ggagatcgag gcgttccggc tgctgaccct gcaaacggcg tggaagatcg accagcacaa
      361 cgactacaag gcggtgcgag ccgacatctc ggcggtaaag gcgatgatgc agaaggtgct
      421 gcatcacgtg tcggccaggg cgctgcagtt gcacggctcg ctgggcacca cccatgagat
      481 gccgttcgtg cagtacctgg tggagtcgtt cgtgctcggg ctcgccgacg ggccgaccga
      541 agtgcacaag gtgacgctgg cgcggctgct gctcaaggac tacaagcccg cgcccgacgt
      601 ctttccctcc cagcacctgc tgcggctgcg ggaggctgcc gaaaagaagt tcgccgacaa
      661 gctggccggg gttccacgca cgtgacccac ggaggttcga tgccgtgtga cggcaaggtg
      721 gccctggtca ccggcaccag ccgaggcctg ggcaaggcga tcgcgcagcg gctggccgcc
      781 gagggcgcca ccgtggcgct gaccgcacgc accatggagc cagacccgaa atacaaaggc
      841 tcgcttcgcc agacgctcga cgagatcgag gcggcgggcg gatcgggggt cgccatccag
      901 gccgacctgt cgaaggccga ggaccgcgag cgcctgttcg gcgaggtcgt cgaacgcgtc
      961 ggcgcgcccg acatcctcgt caacaacgcc gccgtcacgt ttctgcggcc cctcgacgag
     1021 ttcccggagc ggcgggtgcg gctgatgatg gagatgcacg tgctggcacc gttgcacctg
     1081 acgcaactgg cgatcccggc catgcgtcat cgtggccgcg ggtgggtgct caacgtgacg
     1141 tcggtcggcg gtgacctacc cgagggcccg ccgttctccg aattcgaccg cgtcgcgggc
     1201 ttcggcgtct acggcacggt caaggccgcg ctcaaccggc tgacgaagag cctcgccgcc
     1261 gagttgtacg gcgacgggat cgccgtcaac gccgcggcac cgacgaaccc ggtcgccacg
     1321 gaaggggcag gcgcgctgga tctggccaag accgacaccg agggcatcga gctcatcacc
     1381 gagaccgcgt tcctgctgtg caccggcaac ccgacgacgc tgaccggccg catcgcgcac
     1441 acgcaggagt ttctgcgcga agtcggatgg tcatccactt aaataaaccg cttgatttaa
     1501 accgtgccga gatgtctact gaggcggtga cggcagccag agtcttgagc agcgaccgtt
     1561 cgaccgcgac cagggaggcg ctgctactcg cggcggagcg gttgttcgcc gaacgcggga
     1621 tgtacgccgt gtcgaaccgg cagatcagcg aggccgccaa ccagggcaac aacgccgcgg
     1681 cgtgctacca cttcggcacc cggacggacc tgctgagggc catcgaaagc aagcatcgcg
     1741 caccgatcga caagttgcgc ggcaggatgt tggccgacgt cgcgggttcg accgagctgc
     1801 gcgactgggt gggcacgctc gtcctgccgc ttaccgatca cctgacggcc ctcggcagcc
     1861 cgagctggta cgcgcggttc gcggcgcagg cgatggccga tccgtcctac cgccaggtcg
     1921 tcaccaagga cgcgctgaca tcacccgagc tcgtcgcagc gatcaacggc atcaacgact
     1981 gcctgcccga cctaccgaaa cgcgtgcgca tcgaacgaat cgtgatggcg cgcaacctgt
     2041 tgatgcatac ctgtgccgaa cacgaaggcg aactggccga acacggg
//
LOCUS       DASYLX010000260         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_10892, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000260 DASYLX010000000
VERSION     DASYLX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10892"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..1556
                     /locus_tag="VGP27_06410"
     CDS             522..1556
                     /locus_tag="VGP27_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850982.1"
                     /translation="MSGPGRTCTVLLIADPGVPARLADYIADSLPNELADRRSSPVRW
                     ETSVRIEPYLRDEQASLSEVIESVEPSKEDADVVIYMTDLPRRDNTLPVIAEVSVEHR
                     FGLISVAGVGAAFVARRVLGLVVLVTAVVTKQPELRPRSAARLPSAEGSEGVRFFAPR
                     GFRRLRLLAGMVRANRPWRLVVGLSKVLVVALGSGALALATDTIWLFADTMGPWRMCA
                     TTILSIGAMVAWLILDHELWERPKSSAERVRSTLYNTATLVTLTIGVTVLHVALFLSM
                     LFAAAITLMPRVFAQTLGHPVSVADYLSLAWLVASIATVGGALGSGLEDDDGVKAAAY
                     GVRQRQRFDE"
     gene            1767..1955
                     /locus_tag="VGP27_06415"
     CDS             1767..1955
                     /locus_tag="VGP27_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850983.1"
                     /translation="MTTQAGLEKKERDFLAQCVGDAYTMLDLLPGVDPNGPALVWLAD
                     QFLILRRGDCPPAHAMTL"
     gene            complement(2726..3730)
                     /locus_tag="VGP27_06420"
     CDS             complement(2726..3730)
                     /locus_tag="VGP27_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7850984.1"
                     /translation="MRTEDIFDASPRIQTTDVDEATEVLSRVYLPVKLRPAGSNSLDM
                     DLKAEEFPMLTAGYLHFGTDVCIRGDEVPAYYVEAPLSGTALNSWRDGRLEKTTAGSA
                     AVFTPGMPVDLNWSGDCREICIKITEEQMQRQLETMLNRPVRKRITFARSMSLGARAA
                     SDWFGLVRLLAREAGQDDGILAHRLAIDNLQHLLLQGLLLIQPHNYAEALAEEEACAG
                     TAVVRRAIDLMHAYPETPWATAHLARATGVSARALQKAFQRAGQPPPMTYLRRLRLHK
                     AHAELAAHGPNELTVTAVASHWGFLHLSRFAEQYRQVFGESPSETLKNEVNGSAEAPQ
                     "
BASE COUNT          796 a         1339 c         1288 g          820 t
ORIGIN      
        1 cgtcgcggtc atcccactcg atccgcgaca acgcagccaa gttcgacgac atcgcttccg
       61 cagccaccac cccgtcaccc gcaccagccg acacgccgct cacaccaacc cccaccccga
      121 aaaccgaccc ccgacagccg ctatccccgc cacaccacca cgaagactcc ggcatgtccg
      181 gacacggacc gatatgacgt ggcggcaggc gcgcgtcacc acgggccacc ggatgggcat
      241 cccacgccgc cgactcattg cagtactcga attcgccgtc actacatcac cccgcaccgc
      301 cgttgccggc ggtacacaac catcatcgta ctgcggacgt ggggacagca cgcgaagcgc
      361 aacaccaccg atcgccgatc cgcaacacga ttgtggatcg gcgcaagagc ggcatccaac
      421 gcatcaccct gcccagacat gtgtctccat cgtccgtttg agtggctacc gaaagggtat
      481 gtcccgccgg tggtatcgca tgccaccgtc aggagtcggc attgagcgga ccgggtcgca
      541 catgcacggt attgctcatc gccgatccgg gtgtgccggc acgactggcc gactacatag
      601 ccgattcgct gccgaacgaa ctcgccgacc ggcggagttc accggtgcgg tgggaaacct
      661 ccgttcggat cgagccctac ctacgtgatg aacaagcgtc cctttccgag gtcattgaat
      721 ccgtcgagcc gtcgaaggag gacgccgacg tcgttatcta tatgactgac ttgccccgtc
      781 gagacaacac cttgccagtt atcgccgagg tcagcgttga gcaccgtttc ggactcatct
      841 ccgttgccgg cgtcggcgcc gcgtttgtcg cgcgccgcgt gcttggcctg gtcgtactgg
      901 tcacggctgt ggtgacgaaa caacctgaac ttcgaccacg aagtgccgca cggttaccct
      961 cggcagaggg tagcgaagga gtccgcttct tcgccccacg gggctttcgg cggcttcgtc
     1021 tgctggccgg aatggtgcgt gcgaatcggc cgtggcggtt ggtcgttgga ctctcgaagg
     1081 tgctcgtggt tgcgctcgga tccggcgcgc tggcgctcgc aaccgacaca atttggctgt
     1141 tcgccgacac gatgggacct tggcgaatgt gcgcgaccac catcctgtcc atcggagcaa
     1201 tggtcgcctg gttgatcctc gatcacgaac tgtgggagcg tccaaagtcc tccgccgaac
     1261 gggtccggtc aacgctctac aacacggcca ccttggtcac gttgaccatc ggcgtcactg
     1321 tgcttcacgt ggcgttgttc ctgtcaatgc tgtttgcggc cgccatcacg ctgatgccga
     1381 gagtgttcgc tcaaacactc ggtcatccgg tatctgttgc cgactatctg tcgcttgcgt
     1441 ggttggttgc ttccatcgct actgtcggcg gggcgctggg gtcgggactc gaggacgacg
     1501 acggtgtcaa agcagccgcc tacggcgtcc ggcaacgtca gcggttcgat gagtaacgtc
     1561 gacatgacct tcccaacgca tgttcgtcag acgatggttt tcgcgtgaac ggatcgcggt
     1621 gcgaaggcac ttcatctgtc tcccaaggac gattcggatg ctgcttagtc gtgcctgcgg
     1681 gccgacgact gcggccggat cgtgcggcaa ggtaccgaag gggcgggctc gccgcctccg
     1741 taacggtctt gggaaggaat ctcaccatga cgacacaggc tgggctggag aagaaagaac
     1801 gggattttct ggcgcagtgc gtgggtgatg cctacacgat gctagatctg ctcccgggag
     1861 tggatccgaa cggtccggct ttggtgtggc tggcggatca attcctgatt ctgcgtcggg
     1921 gtgactgccc gcctgcgcat gcgatgacgc tgtgatcggc ctcgctaacg tgcgcactgc
     1981 cgcccgaacc ggcgacgcag ccccggttcc ggagtaggtg ctgaagctgc gaaggcggga
     2041 gccgtcgccg gaaccgaacc gctagctcca gatttggtga gagccccccg gacacggtgc
     2101 gcggtcatcg gggtgttgca gcgcttcggt gatcgacgag aaggtttgga acgtcttcgt
     2161 caactcaacg agcttcgggc aagttcggac tgcgcaattc ggcgggacca ccaaggagaa
     2221 tcggatatcg tcttcgtccg ccagctcggc aactgaacgc agcgcgtcga tgccgtccga
     2281 atctatctcg acggtgttgg acaggtttat cacgagcagt tcgggcgtgc ccgttagctg
     2341 gccggccaga gtcctccgga gactagcggc accatcgccc cgcaagtctc cgcaaacgtg
     2401 tacgacggtg ctgttagccg acgaccgctc ccattcgata cggatcttcc acagcttctc
     2461 cggatcatca tcgtttgtcg cgtgtccttc agctaaccga ttcacttgag caagtgaatc
     2521 gacttgccag cacctgcgat atccgcggag cggccaaaac ccatccattt tgcgaaggct
     2581 cccgacatcg acgccactgc aaccgcggcc catccgatcc cacgaccaag acgatcagca
     2641 gctcaagtcg gcacgccgca cgggcgggtc gcacccaagt cggatgcatc ggagcggacc
     2701 gggtgtttcg gaattcatcc cgctgtcatt gaggcgcttc cgcgctcccg ttgacctcgt
     2761 ttttaagcgt ttccgatggg ctttcgccga atacctggcg atattgttcg gcgaaacgac
     2821 tcaaatgcag gaatccccag tgacttgcga ccgcggtgac cgtgagctcg ttcgggccat
     2881 gggctgctaa ttcggcgtgc gccttgtgca gcctcagccg acgcagatac gtcataggag
     2941 gtggctgacc ggcccgctga aaggccttct gtaacgccct ggcgctgacc ccggtggccc
     3001 gggcgaggtg ggcggtggcc cagggtgttt cgggatatgc gtgcatcaga tcgatcgcac
     3061 gtctgaccac cgctgtgcca gcgcaagctt cctcctcggc gagcgcctcg gcatagttgt
     3121 gcggctgtat gagcagcagg ccctggagca acaggtgctg cagattgtcg atcgccagtc
     3181 gatgagcgag gatcccgtcg tcttgcccag cctcgcgcgc cagcaggcgg accaacccaa
     3241 accagtcgct ggctgctcgg gcacctaagc tcatgctgcg ggcaaaggtg attcgtttcc
     3301 gcaccggccg gttcaacatc gtttccagct ggcgctgcat ctgttcttcg gtgatcttga
     3361 tgcagatctc gcggcaatcg ccagaccagt tgagatcgac cggcataccc ggcgtgaaca
     3421 ccgcggccga accggccgtg gtcttttcca atcggccgtc tcgccacgaa ttcaatgcgg
     3481 tgcccgataa tggtgcctcg acgtagtagg caggtacttc atctccgcga atgcagacat
     3541 ccgtgccgaa atgaaggtag cccgctgtca acatcggaaa ttcttcagcc tttaagtcca
     3601 tatccagcga attggatccg gctggccgga gtttcaccgg cagatacacg cgcgacagga
     3661 cctcggtcgc ctcatcgaca tcggtcgtct gaatacgcgg cgacgcgtca aagatgtcct
     3721 ctgtgcgcat ggcaaccgct gactatagtc gctaaaaaat ccctccgcgt gtaatcacgc
     3781 ccggtcgtgg gtagagggcg gccaggcact gccgcccggg aaatgccggc tgagcaccgc
     3841 agcgccaatc ccccgggtcg ctagcggcag aacgatcggc cacggagacg aatccgacgg
     3901 gatcgaatat ccacgaccac cagccagctg ccgcaggcgt taagtcaatg tgcgtaagtc
     3961 ggattccggc gcgctgaacg agtgactgcg gcgtcgagga cggtcgtcct gtcaggacga
     4021 atgaggtttg cgatttctga ccctcggggt gctcgcgaca acggcttcag attgccagac
     4081 tcttggtcgg ggagtcagcg gcgaggggtc ggcatgcagc aatcagtcga ggtagcggat
     4141 caactggtgc acggcgaaac cggcattcat gtcgccatcg cacacttaat ccaaaacgtg
     4201 cgcgcggggg tcgacgtgta tccgggcgcg atatgggcgc taa
//
LOCUS       DASYLX010000261         6811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3302456,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000261 DASYLX010000000
VERSION     DASYLX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302456"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..633)
                     /locus_tag="VGP27_06425"
     CDS             complement(16..633)
                     /locus_tag="VGP27_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7850985.1"
                     /translation="MPTTAPEKLTPKGRATRERIVAAAAELMSRRGVARTTIEDIQEA
                     AAVSTSQMYHYFADKGELVAAVIDFQTDRVLAVQHLGLDRLECLADLRRWRDIMVDIM
                     RRLGFVGGCPIGSLANELAETDPLARAQVARSFVQWENMIRDGLKAIAARGELPDGTE
                     VDNLALATLAAIQGGLLLSQVRRDTAPLEAAVDTMIEHLRTLGVR"
     gene            907..1584
                     /locus_tag="VGP27_06430"
     CDS             907..1584
                     /locus_tag="VGP27_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEV7850986.1"
                     /translation="MTTTANTIITTIADRVAKMDAGRAGQIPDEVLGVFGAEQAGLDA
                     HGIPSGIAEPGDTLGDFDLLDVHGAPTTLSAALGGRTAVVVLYRGAWCPYCNLALRAY
                     QDDLLPTLANNGVALVAISPQKSDGSLSAQEKNALTFTVLSDPGNKIAKRLGVMTAPP
                     DDAQAAQRAMGLDLPEVNADGTYGIPMPTVVIVDPTGVIRWIDVHPNYTTRTEVSDIL
                     AALDTVS"
     gene            complement(1803..5009)
                     /locus_tag="VGP27_06435"
     CDS             complement(1803..5009)
                     /locus_tag="VGP27_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7850987.1"
                     /translation="MDVSGNSGRGLTATGSICAACGTELPPDSRFCNKCGAPVRPAAT
                     VAEYKQVTVLFADVVHSMDIAAVVGAERLREIMTELVNRSSRVVKRYGGTVDKFTGDG
                     IMAVFGAPVALEDHAVRACLAALGIQEEIARLAADVKADDGLDVALRVGLNSGEVIAG
                     EIGSGPFGYTAVGEQVGMAQRMESVASPGGVMLSASTARLVEGAIALGVVELVQIKGA
                     DEPLRAHRLLGVGEQHRVLRRAESSLVGRRWEMSAVEGLLDRAVDGHGAVAALVGPPG
                     IGKSRLVREVAAMAADHAADVFSAFCESHTNQVPFHVVARLLRAATGVEGLDAEAARA
                     QVRAKVRDADTEDLLLLYDLLGIADPDAPLPAIDPDARRRRLTALLNAASLARETPAV
                     YVVEDAHWIDEVSESMIAEFLTVIPQTSSLVLITYRPEYRGVLSRVVDAQTLALASLS
                     DSETAALITELLGADPSVGALGQTITARAAGNPFFAEEIVRELAQRGVLQGQPKAYTS
                     TAEVAGVSVPATLQATIAARIDRLDPKAKRTLGAAAVIGSRFGLDLLTVLGVEAVVND
                     LVATQLIDQVSFTRRPEYVFHHPLTRAVAYESQLKSDRADLHRRVAAAIEAGDATSAD
                     ENAALIAEHLEAAGDLRAAFGWLMRAATWATNRDITAARLSWERATRIADALPADDPD
                     RAALRIAPRTMLCGIAWRVHAHAAGARFEELRELCTAAGDKASLAIAMAGLVMDHVYQ
                     DRMREASQLASEAMALIESLGDPTLTVGLSMPVIYAKMENNKWCDVLRWSQNVIDLAD
                     GDPSKGNFIVGSPLALALTTRAMSGYFLGRGGWRDDLRHGLAMARSADPMSYALVVGY
                     AYSAGIPLGVLRPDDSAVRETEDALQNAERSGDDLALAFTQMALGVALVHCHTAAEHD
                     RGQRLLAEVSEVLLRRGHYLSELPIINVYVAREGARGGERDDAIPLMRSAVDHLFREG
                     QMVAWGVPATGVLVETLLDRGTESDVAEAEAAIERFAAAPADEGLVIREVWLLRLRAL
                     LARAQADEDSYREYRDHYRDMATSLGYEGHMKWAEEMP"
     gene            5290..6486
                     /locus_tag="VGP27_06440"
     CDS             5290..6486
                     /locus_tag="VGP27_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7850988.1"
                     /translation="MGQGASFEVLVVGGGLAGSSLAITLAREGVDVLLVERERVFRDR
                     VRGEVMATWGTVEAKRLGLFELLRERCAIEVRYLTYHVEGVGAPLDLLAAGPHFEPSL
                     AFHHPVMQEALIDEAAVAGATVWRPSKLVSLTAGGPPTAEVMVDGVVKTVTARLVVGA
                     DGRNSRVVHLCGFDRHADPDELLAAGLLVRGDMDTGDAVNMFIGQAAGQIAAVTRIGS
                     DLYRLYFFHHIDAIPGRLSGDRDVGAAFGYLRDAGVPGEWLVDAQPHGPLATFDGAHR
                     WVERPYRDGVVLVGDAAGASDPSWGSGLSRTLRDVRLLRDALTAEKDWRLAADVYAEQ
                     HDEFWERLHDAERLSAAALMSVGPDGASRRARAFEIFDRVPELETWTYGPEAHCDDAV
                     RAELVA"
BASE COUNT         1164 a         2270 c         2165 g         1212 t
ORIGIN      
        1 gccagtacgg ggaatttacc ggacgcccag ggtgcggagg tgttcgatca tggtgtcgac
       61 ggccgcttcc agcggagcgg tgtctcggcg gacctggctg agcagcaggc cgccctggat
      121 ggcggccagc gtcgcgagtg ccaggttgtc gacctcggtg ccgtcgggta gttcgccgcg
      181 agcggcaatg gccttgagcc cgtcgcgaat catgttctcc cactgaacaa acgaccgcgc
      241 gacctgcgcg cgggctagcg ggtcggtttc ggcgagctcg ttggccagcg atccgatggg
      301 gcagccgccg acaaagccca gccgccgcat tatgtcgacc ataatgtcgc gccaccggcg
      361 caggtccgca aggcattcca ggcggtccag gcccagatgc tgaacggcga ggacccggtc
      421 ggtctggaag tcgataaccg ctgcgacaag ttcgcctttg tcggcgaagt agtgatacat
      481 ctgcgaagtg ctcaccgcag cggcttcctg gatgtcctcg atggtggtgc gggccacccc
      541 acgccgggac atcagttcgg cggcagcggc cacgatccgt tctcgcgtcg cgcggccctt
      601 gggagtcagc ttttctggtg cggtggtagg catcggagcg gctttctact ggagcgatcg
      661 catgcggtgt gttggggtgc gaactgaccc gtcacatcag cctctcgtcg atctcgtcat
      721 ggtcctccca ccattggagc ccgatgaaaa tggagtgtca actccaaaat ctgatgtacc
      781 gagtgctcag cagacttttt ggagttgaca ctccattttt ctggatgcaa gctcgcgtca
      841 tgtcgcaaat tcgaaccctg ccggaaaccg ttgtgtggca ccggcacaca tctgaaaggc
      901 tttttgatga cgaccactgc taacaccatt atcaccacga tcgccgatcg cgtcgccaag
      961 atggacgcgg ggagggccgg ccagatcccc gacgaggtac tcggtgtgtt cggcgccgag
     1021 caggccggcc tggatgctca cggcatcccc agcggcatcg ccgaacccgg cgacacgctc
     1081 ggcgatttcg acctgctcga cgtccacggc gcgccgacta ccctgtcggc cgcgctgggc
     1141 gggcgcaccg cggtggtcgt gctctaccgc ggcgcgtggt gcccctactg caatttggcg
     1201 ctgcgcgcct accaggacga cctcctccct accctggcca acaacggcgt tgcgctggtg
     1261 gccatcagcc cgcagaagtc cgacggctca ctgtcggccc aggagaagaa cgcgctgacc
     1321 ttcaccgtgc tgtccgaccc gggcaacaag atcgccaaac ggctcggcgt catgaccgca
     1381 ccccccgacg atgcccaagc cgcgcagcgc gcgatgggtt tagacctgcc cgaggtcaat
     1441 gccgacggca cctacggcat accgatgccc actgtcgtca tcgtcgaccc gaccggggtg
     1501 atccgctgga tcgacgtgca ccccaactac accacccgca ccgaggtcag cgacatcctc
     1561 gccgccctgg acaccgtgtc gtgacacctg cggggcggtc ccccgctcac ccccagcggg
     1621 acccccaccc aatcacggtc gcccgcagcg gtccaaacag taaggcacca agcgtatctc
     1681 aggacttcgt cggccaaaca gcgctgatca tcccagcgct gagatcgacg aaatggtgcg
     1741 tgctactcgc acttctgcgc ccaaacgtac gtttgggcgg tacgcgccgc tgcgcgtaac
     1801 cgtcatggca tttcctcggc ccacttcata tgcccctcgt agccgagcga tgtcgccatg
     1861 tcgcggtagt gatcccgata ctcgcggtac gagtcctcgt cggcctgagc cctggccagc
     1921 agcgcccgca gccgcagcag ccagacttcc cgtataacca gtccttcgtc ggcaggcgcg
     1981 gccgcgaatc gttcgatcgc ggcctcggct tcggccacgt cactctcggt cccccggtcg
     2041 agcagtgtct ccaccaggac acccgtcgct ggaacgcccc atgccaccat ctggccctcg
     2101 cggaagagat ggtcgacggc ggagcgcatg agcggtatgg cgtcatcgcg ttctccaccc
     2161 cgagccccct cacgtgctac gtacacgttg atgatcggta actccgacag gtagtgtccc
     2221 cggcgcagga gcacttcgct gacctcggcc aacagcctct gtccacggtc atgctctgca
     2281 gccgtgtggc agtgcaccag cgcgacgccc agcgccatct gggtgaaggc caatgcgaga
     2341 tcgtcaccgg atcgttcggc attctgtagg gcatcttccg tctcgcgcac cgcggaatca
     2401 tcgggcctta gcacgccaag cggtatccct gcggagtagg cgtagccgac gaccaaggcg
     2461 taggacatcg ggtcggcgct gcgagccatg gccaggccat gccgcaggtc gtctcgccat
     2521 ccgccacgac ccaggaaata gccggacatt gcccgcgtcg tcaacgcgag cgctaacgga
     2581 gacccgacaa tgaagttgcc tttcgacgga tcaccgtcgg ccaggtcgat gacattctgt
     2641 gaccaccgaa gcacgtcaca ccacttgttg ttctccatct tggcgtagat caccggcatg
     2701 gacagcccca ccgtcagggt tggatcgccg agcgactcga tgagggccat ggcttcggat
     2761 gccagctgcg acgcctcgcg catccggtcc tgatacacgt gatccatcac cagccccgcc
     2821 atggcgatcg ccagtgacgc cttgtccccg gcagcggtgc acaactcccg caattcctca
     2881 aagcgggcgc cggccgcatg tgcatggact cgccaggcga tcccgcacaa catggtgcgc
     2941 ggggcaatgc gcagggctgc ccggtccggg tcatcggcgg gtagggcgtc agcgatcctt
     3001 gtggcacgct cccagctcag tcgtgccgcg gtgatgtcgc ggttggtcgc ccacgttgcg
     3061 gcccgcatga gccagccgaa cgccgcccgc agatcaccag cggcctccag gtgctcggcg
     3121 atcaaggcgg cgttctcgtc ggctgaggtc gcgtcccctg cttcgatcgc agcagccacc
     3181 cgccggtgca agtcggcgcg atccgacttg agttgtgatt cgtaggccac cgcacgagtc
     3241 aacgggtggt ggaacacata ctccggtcgt cgggtaaagc tgacctgatc gatgagctgg
     3301 gtcgccacca agtcgttaac gaccgcttcg acgcccagca cagtcaacag gtcgaggcca
     3361 aatcgcgagc cgatcaccgc ggccgcgccc agcgtgcgtt tcgctttcgg gtcgagccga
     3421 tcgatgcgtg ctgcgatggt cgcttgcaag gtggcaggca cactgacacc agcgacttct
     3481 gcggtcgagg tgtatgcctt gggctgtccc tgcagcacgc cacgctgggc cagttcgcgc
     3541 acgatctcct cggcgaagaa cggattaccg gcggcccgag cggtgatcgt ctggcccaat
     3601 gcgccaacag atgggtcggc gccgagaagc tccgtgatca atgctgccgt ctccgaatca
     3661 cttagcgacg caagcgctag ggtctgcgca tctacaaccc ggcttaacac gccccgatat
     3721 tccgggcggt aggtgattag caccagcgag gatgtctgcg ggatcaccgt gaggaactcg
     3781 gcgatcatcg actcgctgac ctcatcaatc caatgagcgt cctcaacgac gtagaccgcg
     3841 ggcgtctccc gagccaacga agcggcattc agcagcgccg tcaaccgtcg ccgacgggca
     3901 tcgggatcga ttgctggtaa cggagcatcc gggtcggcga tgcccagtag gtcatagagc
     3961 aacagcaagt cctcggtgtc tgcgtcgcga accttggcgc gaacctgggc gcgggccgcc
     4021 tccgcgtcaa gaccctcgac gccggtggcc gcgcgcagca gccgcgctac gacatggaag
     4081 gggacctggt tggtgtgtga ttcgcaaaag gcgctgaaca cgtccgcagc gtggtcggct
     4141 gccatcgcgg cgacctcgcg caccagacgg ctcttgccaa tgccaggtgg ccccaccagg
     4201 gcggccaccg cgccgtggcc atcgaccgcg cggtccaaca agccttcgac ggcggacatc
     4261 tcccaccgcc gaccgaccag actcgactca gcacgcctga gaacgcggtg ctgttctccc
     4321 acgcccaaca gccgatgggc gcgcagcggc tcgtcggcac ccttaatctg gacaagttca
     4381 accacgccca gagctattgc gccctcaacc agtcgtgcgg tggatgcgct gagcatcacc
     4441 ccaccgggtg aggcgaccga ctccatccgc tgagccatcc cgacctgttc accaacggcg
     4501 gtgtaaccga aaggccccga gccgatctca ccggctatca cctcaccgga attgagcccc
     4561 acacgcaggg caacgtcgag gccgtcatca gctttgacgt cagcggccag ccgggcgatt
     4621 tcttcttgaa tgcccagcgc ggcgaggcag gcccgcaccg catgatcctc caacgccacc
     4681 ggagcgccga acactgccat gatcccgtca ccggtgaact tgtccaccgt cccgccataa
     4741 cgcttcacta cccgcgacga gcggttgacc agctcggtca tgatctcgcg cagccgctcc
     4801 gccccgacga ccgcggcaat atccatagag tgcacgacgt cggcgaacag caccgtcacc
     4861 tgcttgtact ccgcgaccgt cgcggccggc ctgactggcg cgccgcactt gttgcagaac
     4921 cgtgagtccg gcggcaactc ggtgccacac gcggcgcaga tcgaaccggt tgcggtcagc
     4981 ccacggccac tattcccgct gacgtccacg ccagtatcgt gtcaccgctg gtcagcgatg
     5041 ttcgtcatat cgccgcgtct ccgagacgaa gttttcagac tcataactcg tctattacgc
     5101 gacagcctct gtgggttcgt ttgcgctccc ggcgcccagt tgcggaaaca gcggcatcgg
     5161 ccgtgcgccg agttccggcc gcgtcacctc gatcgcggcc ccgcgagcac ttcgaaggtc
     5221 gccgcggacc gtctcgtcga tcccgacgtc cagattgtcc gaggcgcgaa ggagcttagg
     5281 ctggtcggtg tgggccaggg tgcgagtttc gaggtacttg tggtcggcgg ggggcttgct
     5341 ggttcctcgt tggcgatcac gttggcgagg gagggcgtgg acgttctgct ggtcgagcgt
     5401 gagcgggtct tccgggaccg tgtcaggggg gaggtgatgg ctacgtgggg gacggtcgag
     5461 gcgaagcggc ttggtctatt tgagctattg cgggagcggt gtgccatcga agtccggtat
     5521 ctgacttacc acgtcgaggg tgtcggcgcg ccgctagatc tgctggcggc cggaccgcat
     5581 ttcgagccgt cgcttgcgtt tcatcacccg gtgatgcagg aggctctgat cgatgaggcg
     5641 gcagtcgcgg gggcgacggt ctggcgtccg tcgaaattgg tatcgctgac ggccggtggc
     5701 ccgcccacgg ccgaggtcat ggtcgacggt gtggtgaaaa cggtcacagc acgtctggtt
     5761 gtcggtgcgg acggtcgcaa ctcgcgggtt gtgcacctgt gtgggttcga ccggcacgcc
     5821 gacccggacg agttgctggc cgcggggctg ctggtgcgag gagatatgga cacaggcgac
     5881 gcggtcaaca tgttcatcgg ccaagccgcc gggcagattg ccgcggtcac ccggatcgga
     5941 tctgacctct accggctcta cttcttccat cacatcgacg cgatccccgg caggctctcg
     6001 ggtgaccgcg acgtcggggc cgctttcggg tatttacgcg atgcgggtgt gccgggtgag
     6061 tggctggtgg atgcgcaacc gcacgggccg ctcgccacgt tcgatggcgc ccaccggtgg
     6121 gtcgaacggc cgtatcggga cggtgtcgtc ctcgtcggtg atgctgcggg tgcgagcgac
     6181 ccgtcctggg gaagcggtct gtctcgcacg ctacgcgacg tccgcctgct ccgggacgcg
     6241 ctgactgcgg aaaaggactg gcggcttgca gcagatgtgt acgcggaaca gcacgacgag
     6301 ttctgggagc ggctgcacga cgccgagcgc ctttccgctg cggcgctaat gtcggtcggc
     6361 ccagacggtg cctcgcgccg tgcccgggcc ttcgagatct ttgatcgcgt acccgaactc
     6421 gaaacctgga catacggccc ggaggcccac tgcgacgacg ctgtccgcgc ggaactagtc
     6481 gcttaagccc tgagcgcgtt ctgcggcaga taattgagac gctcagaact cgtctatttc
     6541 gggactgttg tgtctaggcc ggttcgcagg tgccatgcgg gtacccgcag cgccgagatc
     6601 gacgaaatgg tgcgtgctac tcgcactttt gcgcccaaac gtacgtttgg gcgtcacgcg
     6661 ccgctgcggc cgccgatacc ttcgtgatcg acgtcgcgcc agaagtcgat gtcgtactgc
     6721 gtgtccggga tctggatcat ctcgacggac ggggtgagcc cacgggccat ttcgagctct
     6781 tcggcgtcga tgtcgagccg gtcgacgccg t
//
LOCUS       DASYLX010000262         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_10948, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000262 DASYLX010000000
VERSION     DASYLX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10948"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGP27_06445"
     CDS             complement(<1..1216)
                     /locus_tag="VGP27_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006372600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7850989.1"
                     /translation="MNRNTGPQMSRRNMLAALGLAGAAAASVPILSSCGVGGKAALPN
                     GAASVTGGFDWKKASGSTINILQTPHPYQVSYQPLLKEFTELTGITVNVDLVPEADYF
                     TKLNTELAGGSGKHDAFMLGAYFIWQYGPPGWIENLDPWLKNSSATNADYDFDDIFDG
                     LRTSTRWDFTLGHPLGTGGQWAIPWGFENNVVAYNKAYFDQRGIKKLPDRLDDFIQLA
                     VDLTDRSQNRYGISTRGSKSWATIHPGFMTQYTREGAVDYTFNGSDLIAEMDSDKAIA
                     FTEKWIEMQHKAGPTSWTTYDYPNATGDLGDGKAMMVFDADSATYPKNKPGASKEAGN
                     LAWYPGPAGPDGNYKTNLWTWSWAMSANSRNKLPAWLFIQWATGKDSMNKAVQGGKYA
                     DPVRKSVFDTTFK"
     gene            complement(1238..2326)
                     /gene="eltD"
                     /locus_tag="VGP27_06450"
     CDS             complement(1238..2326)
                     /gene="eltD"
                     /locus_tag="VGP27_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythritol/L-threitol dehyrogenase"
                     /protein_id="HEV7850990.1"
                     /translation="MSDQIPEKMQAVVCHGPEDYRLEEVAVPVRKPGEALIRVEAVGI
                     CASDLKCFHGAAKFWGDENRPAWAETMVIPGHEFAGRVVELDDAAASRWGIAVGDRVV
                     SEQIVPCWECRYCKRGQYHMCQPHDLYGFKRRTPGAMASYMVYPAESLVHRVSGDIPA
                     HHAAFTEPLSCSLHAVERAGITFEDTVVVAGCGPIGLGMIAGARAKSPMHVVALDMEP
                     GKLELAKRCGADIAINIAEQDAVAMIKDLTGGYGADVYLEGTGHPSAVPQGLNALRKL
                     GRYVEYGVFGSDVSVDWSIISDDKELDVLGAHLGPYCWPAAIRMIESGVLPMDDICTH
                     QLPLPEFQKGLDLVASGKESVKVSLIPA"
     gene            2443..3480
                     /locus_tag="VGP27_06455"
     CDS             2443..3480
                     /locus_tag="VGP27_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEV7850991.1"
                     /translation="MPRSAPAASAEQTADSGAVELEGSHFPASLLYTAARLYYEDDAT
                     QADVAAKLGTSRATVSRLLAEAKRQGIVRIEVVPPAEARPGDLGDQLARALNLENVYL
                     APPLPAPGAGRSLVDVMGRPLAPAVGRALSEAGLLPGDVLLVSSGRTVYEVAQHELPA
                     LPGVIIAPTVGGNDQAEDWYQTNEITRLVANRVGGRANYLFAPALPGPQLYRSLLNDP
                     SIQRVLGLWPHARCALMGVGAPPLMRSDIPGFVPTASSSLRSAVGDVCSRFFDRDGEP
                     VAFEGGERLIAVELEALRHIPVTIGVAVGKEKVESIIAGARGGYFNRLVTDPVTAHDI
                     LASPAVAEQAS"
BASE COUNT          582 a         1147 c         1098 g          689 t
ORIGIN      
        1 tcttgaaggt ggtgtcgaac accgacttcc gcaccgggtc ggcatactta ccgccctgta
       61 cggccttgtt catcgagtcc ttgccggtgg cccactggat gaacagccag gcaggtagct
      121 tgttgcgcga gttcgcgctc atcgcccaac tccatgtcca caagttggtc ttgtagttgc
      181 catcgggtcc ggcgggtccc ggataccacg ccaggttgcc tgcttccttg ctggcacccg
      241 gtttgttctt cgggtaggtc gcgctgtcgg catcgaagac catcatcgcc ttgccgtcac
      301 cgagatcgcc tgtggcgttg ggatagtcgt aggtcgtcca ggaggtcggc ccggccttgt
      361 gctgcatctc gatccacttc tcggtgaacg cgatggcctt gtcactgtcc atctccgcga
      421 tgagatccga cccgttgaac gtgtagtcca ctgcgccctc acgcgtgtac tgcgtcatga
      481 acccggggtg gatggtggcc cacgatttcg aaccgcgggt ggagatgccg taacggttct
      541 gcgatcggtc ggtgaggtcg acggccagct ggatgaaatc gtcgagcctg tcgggcagct
      601 tcttgatgcc gcgctggtcg aagtaggcct tgttgtaggc caccacgttg ttctcgaagc
      661 cccatgggat cgcccactga ccccccgtgc ccagcggatg gccgagggtg aaatcccacc
      721 gggtcgaagt gcgcagaccg tcgaagatgt cgtcgaagtc gtaatctgcg ttggtggccg
      781 acgaattctt cagccacggg tcgagattct cgatccagcc cggtggtccg tactgccaga
      841 tgaagtaggc gccgagcatg aaggcgtcgt gcttgcctga gccaccggcc agttcggtgt
      901 tgagtttggt gaaatagtcc gcctctggaa cgaggtcgac attgacggtg atcccggtga
      961 gctcggtgaa ctccttgagc agcggctgat acgacacctg gtagggatgc ggggtctgca
     1021 gaatgttgat cgtcgagccc gacgcctttt tccagtcgaa gccgcccgtg acggaggccg
     1081 ccccgttcgg cagagcggcc ttcccgccga cgccgcagga actgaggatg gggacgcttg
     1141 ccgcggccgc accggcgagt cccaacgccg cgagcatgtt tcgccgcgac atctgcggcc
     1201 cggtgtttcg gttcatggtg tcagttgccc ttccgcatca ggcggggatc agggagactt
     1261 tgacggattc cttgccgctg gcgacgaggt cgagaccctt ctggaattca ggcaatggca
     1321 gttggtgtgt gcagatgtcg tccatcggca gcacgccgga ctcgatcatt ctgatcgctg
     1381 ccggccagca gtaaggcccg agatgggcgc cgagcacgtc gagttccttg tcgtcgctga
     1441 tgatgctcca gtcgacagac acgtcgctgc cgaacacccc gtattcgacg tagcggccga
     1501 gcttgcgtaa tgcgttgaga ccctgtggaa ccgcggaggg atggccggtc ccctcgagat
     1561 acacgtctgc gccgtagccg cccgtcaggt ccttgatcat cgcgacggcg tcctgttcgg
     1621 cgatgttgat ggcgatgtca gcgccgcaac gcttggccag ctcgagcttc cccggttcca
     1681 tgtcgagagc gaccacgtgc atcggactct tggcccgcgc gcctgcgatc atgccaaggc
     1741 cgatcggccc gcacccggcg accaccacgg tgtcctcgaa ggtgatgccg gctcgctcca
     1801 cggcgtgcag ggagcacgat agcggttcgg tgaacgcggc gtgatgggcc gggatatcgc
     1861 cggataccct gtgcaccagc gattccgccg gatagaccat gtagctcgcc atcgcaccgg
     1921 gagtgcgccg cttgaatccg tagagatcgt gcggctgaca catgtggtac tgaccacgct
     1981 tgcaatatcg gcattcccag cacggcacga tctgctccga caccacccga tcgcccaccg
     2041 cgatacccca gcgagacgcc gccgcgtcat cgagttcgac cacccgcccg gcgaattcgt
     2101 gtccggggat caccatggtc tcagcccatg cgggccggtt ctcgtcaccc cagaacttcg
     2161 ccgccccgtg aaagcacttg aggtcgctgg cgcagatacc gacggcttcg accctgatca
     2221 gcgcctcgcc cggcttacgt accgggaccg ccacctcttc gagtcggtag tcctcgggtc
     2281 catgacagac cacggcctgc atcttctcgg ggatttgatc gctcatcagc actccttcaa
     2341 ctgtggttgg agtcacacta tcgaactccc tgcacatttg tccagaacga atgttcagca
     2401 aaattcttcc cagaacagat gttcagtgcc atactcgacg ccatgcctcg atcggctccg
     2461 gcagcatcgg ctgagcaaac cgccgacagc ggcgccgttg agctggaagg cagtcatttt
     2521 cctgcgtccc tgctgtacac agccgcgcgc ctgtactacg aggatgacgc cacccaggcc
     2581 gacgtcgctg cgaagctggg caccagccgg gcaaccgtca gccggctcct cgcggaggcc
     2641 aaacggcagg gcatcgttcg aatcgaggtg gttcctcccg ccgaggcgcg gccaggtgat
     2701 cttggcgatc aactggccag ggcgttgaac ctcgagaacg tgtacctcgc ccctcccctt
     2761 cccgcacctg gcgctggccg ctcactggtg gacgtgatgg gccgcccgct ggctcccgcg
     2821 gtcggacgcg cgctcagcga agccggactg ctgccaggcg acgttctcct ggtctcgtcg
     2881 ggtcgcaccg tctacgaggt cgcccagcac gagctgcctg cactgccggg tgtcatcatc
     2941 gccccgacag tgggcggcaa cgatcaggcc gaggactggt atcagaccaa cgagatcacc
     3001 cgcctggtcg ccaatcgtgt tggcggcagg gcgaattacc tcttcgcccc tgcgttgcca
     3061 ggaccgcagc tataccgttc tctgctcaac gaccccagca tccagcgtgt gctcggcctg
     3121 tggccgcacg cgcgctgcgc gctcatgggt gtcggcgccc cgcccctgat gcggtcggat
     3181 attccaggat tcgtgccgac cgcgtcctcg tcgctgcgct ccgcggttgg cgacgtgtgc
     3241 tcgcgattct tcgaccggga cggtgagccg gttgccttcg aaggaggtga gcggctgatc
     3301 gcggtggaac tcgaagccct gcgacacatt ccggtcacca tcggggtcgc cgtcggcaag
     3361 gaaaaagtgg aatccatcat cgcgggcgca cgcggcggct atttcaaccg tctcgtcacc
     3421 gacccggtga ccgcgcacga catcctggcc agccccgccg tcgcggaaca ggcgtcctga
     3481 cgtggcggct gtgctcgatg gcaagcgggc ccttat
//
LOCUS       DASYLX010000263         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1656905,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000263 DASYLX010000000
VERSION     DASYLX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656905"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGP27_06460"
     CDS             <1..586
                     /locus_tag="VGP27_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306775.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV7850992.1"
                     /translation="GSEKVPGLLDEEGFSKDSKTETFAAITVEIDTRRWAGVPFFLRT
                     GKRLGRRVTEIALVFKRAPHLPFDSTMTEELGQNALVIRVQPDEGITTRFGSKVPGTQ
                     MEVRDVNMDFSYGAAFAEDSPEAYERLILDMLLGEPSLFPVNREVEYSWEILDPVLDY
                     WAQHGRPEPYEAGTWGPKSAFEMIRRLGREWRRP"
     gene            583..1494
                     /gene="opcA"
                     /locus_tag="VGP27_06465"
     CDS             583..1494
                     /gene="opcA"
                     /locus_tag="VGP27_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEV7850993.1"
                     /translation="MIVNLPGATTNDINKKIINLREEGGAVTMGRVLTLVIVPDSEDI
                     LEDSVAAANFASREHPCRVIAVLPGDRKAAEPRLDAEIRVGADAGAGEVVVLRLAGEL
                     ADHANSVVLPFLLPDTPVVAWWPEGGPAVPAQDPLGQLAIRRITDATNSSDPLAAIKS
                     RLQGYTAGDTDLAWSRITYWRALLASALDQAPFEPIESALVSGLKTEPALDILAGWLA
                     SRIDGPVQRAVGDLKVELHRPSETVTLSRPQTGTTATLSRTSRPEARIPLARRDAKEC
                     LAEDLRRLDADEIYFEALGGIDKVQYV"
     gene            1491..2234
                     /gene="pgl"
                     /locus_tag="VGP27_06470"
     CDS             1491..2234
                     /gene="pgl"
                     /locus_tag="VGP27_06470"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV7850994.1"
                     /translation="MSTIVEKYSDKAALVAAAGDRLIGAITDAIAHRGRALVALTGGG
                     TGIGMLKHVRDNGGAIDWSKVQLFWGDERFLPADDDERNEKQAREALLNHIDIPAANV
                     HPMAASDGEFGDDIDAAASAYERVLAASASDGEPAPDFDVHLLGMGGEGHVNSLFPHT
                     AATRETARQVVAVTDSPKPPPRRITLTLPAVQRSREVWLVAAGAEKADAVAAAIGGAD
                     PDELPAAGAKGREATVWLLDDEAASKLDS"
     gene            2487..>2721
                     /locus_tag="VGP27_06475"
     CDS             2487..>2721
                     /locus_tag="VGP27_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV7850995.1"
                     /translation="MADKGGRTGARSGRERIQKLAQAALNADVTVDQVDAILAGLGET
                     LEDLNNSTGNLDTTLERFIDTISRINELAPRLNA"
BASE COUNT          482 a          865 c          946 g          428 t
ORIGIN      
        1 gggcagcgag aaggtgccgg gccttctcga tgaggagggc ttctccaagg actccaagac
       61 cgagacgttc gcggcgataa ccgtggagat cgacaccagg cgatgggccg gtgtgccgtt
      121 cttcttgcga accggaaagc gattggggcg cagggtgacc gagatcgcgc tggtgttcaa
      181 gcgcgccccg catctgccgt tcgacagcac gatgacggag gaactgggcc aaaacgcgtt
      241 ggtgattcgg gtacagccgg acgagggcat caccactcgc ttcgggtcga aagtgcccgg
      301 cacccagatg gaggtccgcg acgtcaacat ggatttctcc tacggcgcgg cgttcgccga
      361 ggactcaccg gaggcatacg aacggctgat cctcgacatg ctgctcggtg agccgtcact
      421 gtttccggtc aacagagagg tcgaatactc ctgggagata ctggatcccg tcctcgacta
      481 ttgggcccag cacggcaggc cagaaccgta tgaggccggc acctggggac ccaagtcggc
      541 gttcgaaatg attcgtcgct tgggccggga atggaggcgg ccgtgatcgt caatctgcct
      601 ggcgccacca ccaacgacat caacaagaag atcatcaacc ttcgggaaga ggggggcgcg
      661 gtcaccatgg gccgcgtgct gaccctggtc atcgttcccg actccgagga cattctcgag
      721 gactccgtcg cggcggcgaa cttcgccagc cgcgagcatc cctgccgggt gatcgcggta
      781 ctgccgggcg atcgcaaagc cgcagagccg cgactcgacg ccgagattcg ggtcggcgcg
      841 gatgcgggtg cgggcgaggt cgtggtgttg cgccttgccg gtgagctcgc cgaccatgcc
      901 aacagcgtgg tgctgccgtt cctattgccg gacacgcccg ttgtggcgtg gtggcccgag
      961 ggcggtcccg cagttccggc acaggatccg ctcggtcagt tggcgattcg acggatcacc
     1021 gatgcgacca acagcagtga cccgttggcc gcgatcaaga gcaggctgca gggctacact
     1081 gcgggcgaca cggatttggc gtggagtcgc atcacctact ggcgtgctct actcgcctcg
     1141 gcactcgacc aggcgccgtt cgagccgatc gagtcggcgc tggtgtcggg cctcaagacc
     1201 gaacccgcgc tcgacatcct tgcgggctgg ttggccagcc ggatcgacgg cccggtacag
     1261 cgggcggtgg gcgacctgaa ggtcgaactg caccggccaa gcgagaccgt gacgttgagc
     1321 cgtccgcaga cgggtaccac cgcaaccctg agtcgaacga gcaggccgga agcgcggatt
     1381 ccgttggccc gcagggatgc caaggaatgt ctggccgagg acctgcgccg gttggacgcc
     1441 gacgagatct acttcgaggc gctcggcggc atcgacaagg tgcagtacgt gtgagcacca
     1501 tcgtcgagaa gtattcggac aaggcggcgc tcgtcgccgc cgccggcgac cgcctcatcg
     1561 gtgccatcac cgacgccatc gcccacaggg gccgagcctt ggtcgcgctg accggtggcg
     1621 gcacgggaat cggcatgctc aaacacgttc gggacaacgg cggcgcgatc gactggtcga
     1681 aggtgcagtt gttctggggc gacgagcgct tcctgcccgc ggacgacgac gagcgcaatg
     1741 agaagcaggc tcgcgaggca ctgttgaacc acatcgacat ccccgctgcc aacgtgcacc
     1801 cgatggccgc cagcgacggt gagttcggcg acgatatcga cgccgcggcg tcggcctacg
     1861 aacgagtgct ggccgccagc gccagtgacg gcgagccggc accggatttc gacgtccatc
     1921 tgctcgggat gggcggggag ggccatgtca actcgttgtt tccgcacacc gccgcaaccc
     1981 gcgagaccgc tcggcaggtt gtcgctgtca ccgactcccc caagccaccg ccgcggcgaa
     2041 tcacgctgac gctgcccgca gttcagcggt cgcgcgaggt gtggctagtg gcggccggtg
     2101 ccgagaaggc cgatgcggtg gccgcggcga tcggtggcgc cgacccggat gaactgcccg
     2161 ccgcgggagc gaagggccgc gaagcgacgg tgtggctgct cgacgacgag gcggcgagca
     2221 agctcgactc ctgacctcgg tcggggccgc gccggcgcgt cccgggcgcg gcgggaccgg
     2281 tgcgacggcg gctcctattc ggtgctcaac ggcgcgtcgg cgctgtccga ctctccgtcg
     2341 gcgaagccgg cggtgtagtc ggacagctgt ctctccgtgt cacggtcggt cgtccaccgg
     2401 aggcggctct ggcactgttt gcgctgcatt caggtcagac gaccggtcgg ccaccacggt
     2461 tccagcggtg tggtcaaatg accgtcatgg cagacaaggg cggcaggacc ggtgctcgca
     2521 gcggccggga gcgaattcag aaactcgcgc aggcggcgtt gaatgccgat gtcacggtcg
     2581 atcaggtcga cgccatcctc gccggtctcg gtgagacact cgaggatctc aacaattcaa
     2641 cgggcaatct cgatacgacc ctggagcggt tcatcgacac gatcagccgc atcaacgaac
     2701 tggcaccacg cctgaacgcg g
//
LOCUS       DASYLX010000264         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3850164,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000264 DASYLX010000000
VERSION     DASYLX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850164"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /gene="topA"
                     /locus_tag="VGP27_06480"
     CDS             complement(<1..1703)
                     /gene="topA"
                     /locus_tag="VGP27_06480"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV7850996.1"
                     /translation="MADEDRGSGRSGGVRRLVIVESPTKARKIAGYLGSNYIVESSRG
                     HIRDLPRNAADVPAKYKSEPWARLGVNVEDNFEPLYIISPEKKTTVAELKDLLKNVDE
                     LYLATDGDREGEAIAWHLLQTLKPRVPVKRMVFHEITEPAIRAAAEDPRDLDNDLVDA
                     QETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVSRERERMAFRSAGYWDV
                     TAELDASVSDAQASPPTFVAKLNTVDGRRVATGRDFDSLGQLKKPDEVLVLDESSAGA
                     LATGLRGAQLEVTSVEQKPYTRRPYAPFMTSTLQQEAGRKLRFSSERTMSIAQRLYEN
                     GYITYMRTDSTTLSESAITAARNQAGQLYGEEYVHPTARQYTRKVKNAQEAHEAIRPA
                     GDVFQTPGQLHSQLDTDEFRLYELIWQRTVASQMADARGTTLSLRISGAARPATSESG
                     QSTDGQQVIFNASGRTITFPGFLKAYVESIDELAGGESDDAESRLPNLQQGQRVDAKE
                     LTPDGHNTTPPARYTEASLIKSLEDLGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSW
                     VA"
     gene            complement(1838..2416)
                     /locus_tag="VGP27_06485"
     CDS             complement(1838..2416)
                     /locus_tag="VGP27_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7850997.1"
                     /translation="MSQLSFFSAESVPPAVADLTGLLAAPGQVVVVGEGARLSVVVDG
                     PWRAVALAEMIVDSGLEPEIARTDENTPLVRTAVDSRLLRIAAAWTRGAVKTVPPQWL
                     PGPRELRAWTLAAGTPEADRYLLGLDPHAPDTHSPLASALMRVGIAPTLIGTRGSHPA
                     LRISGRRRLSRLVENVGEPPGDVEAFSLWPRI"
     gene            complement(2539..2742)
                     /gene="cspA"
                     /locus_tag="VGP27_06490"
     CDS             complement(2539..2742)
                     /gene="cspA"
                     /locus_tag="VGP27_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HEV7850998.1"
                     /translation="MPQGTVKWFNAEKGFGFIAPEDGSADVFVHYTEIQGSGFRTLEE
                     NQKVEFEVGQSPKGPQATGVRSI"
     gene            3010..>4553
                     /locus_tag="VGP27_06495"
     CDS             3010..>4553
                     /locus_tag="VGP27_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7850999.1"
                     /translation="MSDFGRELLACAVEGTDPEEKPLRHVADLPPRQARPRAWPHWAD
                     PDVVRAFIDRGIAAPWSHQSEAAGLAHDGRHVVLSTGTASGKSLAYQLPILTAFATDP
                     RARALYLSPTKALGHDQLRTAHALTDAVSKLGTVAPSAYDGDSPTEIRRFARERSRWI
                     FSNPDMIHLSILRNHARWAVFLRNLRYIVVDECHYYRGVFGSNVAMVLRRLLRLCTRY
                     SAAPTVIFASATTASPAATASELIGQTVLEVTEDGSPQGARTVALWEPALRTDMVGEN
                     GAPVRRSAGAEAARVMADLVAEGARTLTFVRSRRGAELTALGARSRLEEVAPDLASKV
                     ASYRAGYLAEDRRELERALAHGDLRGLASTNALELGVDIAGLDAVVLAGFPGTVTSFW
                     QQAGRSGRRGQGALILLIARDDPLDTYLVHHPEALLDKPIERVVIDPGNPYVLGPQLL
                     CAATELPLTDAEVRAWGAEAVAETLVDDGLLRRRPSGYFPSPGVDPHPAVDIRGSSGG
                     QIAILET"
BASE COUNT          726 a         1527 c         1491 g          809 t
ORIGIN      
        1 gccacccacg acggcaccag cgcgctgccc ttcttgtgga cgtagccgcg gtcctggatg
       61 gtcttgatga tcgacgagta cgtcgacggc cggccgatgc caagatcctc cagcgacttg
      121 atcagcgacg cctcggtgta ccgggcgggc ggtgtcgtgt tgtgaccgtc gggcgtcagc
      181 tccttggcgt cgacgcgctg gccctgctga agattcggca gccggctttc ggcgtcgtcg
      241 gattcgccgc cggccagctc gtcgatgctc tcgacgtagg ccttcaggaa gccggggaac
      301 gtgatggtgc gcccgctggc gttgaagatg acctgctggc cgtcggtcga ctggcccgat
      361 tctgatgtcg ccggccgagc tgctccggag atgcgcaacg acagcgtcgt gccgcgcgca
      421 tcggccatct gcgaggccac ggtgcgctgc cagatgagct cgtagaggcg gaactcgtcg
      481 gtgtcgagtt ggctgtgcag ctgacccggg gtctggaaca catcaccggc gggccggatc
      541 gcctcgtgcg cctcctgggc gttcttcacc ttgcgcgtgt actggcgcgc cgtggggtgc
      601 acgtactcct cgccgtacag ctggccggcc tggttgcggg cggcggtgat ggccgactcc
      661 gacagcgtcg tcgagtcggt acgcatatag gtgatgtagc cgttctcgta gagccgctgc
      721 gcgatgctca tcgtgcgctc cgatgagaac cgcagcttgc ggccggcctc ctgctgcagc
      781 gtcgacgtca tgaacggcgc gtaaggccta cgggtgtaag gcttttgctc aaccgaggtg
      841 acttcgagct gcgcgccgcg cagccccgtc gccaacgccc cggcactgga ttcgtcgaga
      901 accagcacct cgtcgggctt cttgagctga cccagcgagt cgaagtcgcg accggtggcc
      961 acccgcctgc cgtcgacggt gttgagcttg gcgacgaagg tcggcggaga cgcctgcgcg
     1021 tcggagacgc tggcgtcgag ttcggcggtg acgtcccagt agccggcgct gcggaacgcc
     1081 atccgctcgc gttcgcgcga gacgatgatg cgggtcgcca ccgactggac gcggccggcc
     1141 gacagcttcg gcgcgacctt cttccacagc acggggctga cctcgtagcc gtacagccgg
     1201 tccaggatgc ggcgggtttc ctgcgcgtcg accaggtcgt tgtccaggtc ccgcggatcc
     1261 tctgcggccg cccggatcgc gggttcggtg atctcgtgga acaccatccg cttgaccggc
     1321 accctcggct tcagggtttg cagcagatgc cacgcgatgg cctcgccctc acggtcgccg
     1381 tcagtggcca gatacagttc gtcgacattc ttgagcaggt ctttcagctc ggcgaccgtg
     1441 gtcttcttct ccgggctgat gatgtagaga ggctcgaagt tgtcctcgac gttcacaccg
     1501 aggcgcgccc acggctccga cttgtacttc gcaggcacgt cggcggcgtt gcgcggcagg
     1561 tcccggatgt gtccgcggga cgactcgacg atgtagttcg agcccaggta acccgcaatt
     1621 ttgcgtgctt tagtaggcga ctcgacgatt acgagtcgcc gcacaccccc gctgcggccg
     1681 ctgccgcggt cctcgtcagc cacctgtgct tacgctccac ttcatatgtt gccgttcgac
     1741 gcgaattcat gcgttctcga ctatccgtcg ccgttggcac ctgacaattt cgcaccccgg
     1801 acgagccgac gcaaactgac cctccggggc cggggcccta gattcggggc cagagcgaga
     1861 acgcctctac gtcgccagga ggttccccca cattctctac caggcgtgat agccgtcggc
     1921 gaccgctgat ccgcagtgcc ggatgcgagc ctctggtgcc gatgagagtc ggtgctatcc
     1981 cgacccgcat cagggccgag gcaaggggtg agtgagtgtc gggagcgtgc ggatcgagtc
     2041 ctaatagata acggtcggct tcgggcgttc cggccgccag cgtccacgcc cgcagctcgc
     2101 gcgggccagg cagccactgc ggtgggacgg tcttgaccgc tccccgtgtc caggcggcgg
     2161 cgatgcgcag cagccggcta tccaccgcgg tgcggaccaa cggggtgttc tcgtctgttc
     2221 gggcgatctc gggctcgaga ccggaatcga cgatcatctc ggccagggcg accgcacgcc
     2281 acgggccatc caccacaaca gacagccgtg cgccctcacc gaccacgacc acctggccgg
     2341 gcgcggcgag caaccccgta aggtcggcga ccgcaggcgg caccgactct gccgagaaga
     2401 aggacagctg gctcacggag acgacagtag gccagtaccg cgctgccgtg ctgtcggcgg
     2461 ggccgcagtc gcgcccagaa acgaaaaccc ccgccggccc tcaatggact gtgcaggggg
     2521 attcgtcagg ttcgatactt agatggaccg aacgccggtt gcctgtgggc ccttggggct
     2581 ctgaccgacc tcgaactcaa ccttctgatt ctcttcgaga gtccggaagc cggatccctg
     2641 aatttccgtg tagtggacaa acacatcggc ggagccgtcc tcgggagcga taaagccgaa
     2701 gcccttctcc gcgttgaacc acttcacagt tccctgtggc atctttcgat ctttccttct
     2761 cttcttaccg ggagcggtcc accgacttcc ggtgcaccgg gcccgttccg accgccatac
     2821 caacgcgtgt gtcgccggaa cttgacccga cctacaaccc tcgcaggaac cgcgaccgca
     2881 acgtcgatcc tgcgagtgca tatacacgaa cacagaagct gcgaccgccc ttagtcaatc
     2941 atgttcgaag ctccagcgac agtcttgttg cagcaagtag ggaacaattc atccacagga
     3001 gggccaacga tgtcggattt cggccgcgag ctgctcgcct gcgcggtcga gggcaccgat
     3061 ccggaggaga agccgctgcg ccatgtcgcg gacctgccgc cgcggcaggc gcgaccacgg
     3121 gcatggccgc actgggccga tccggatgtg gtgcgggcgt tcattgatcg cggaatcgcc
     3181 gcgccctggt cacaccaatc cgaggccgcc ggcctcgccc acgacggccg tcacgtggtg
     3241 ctcagcaccg gcacggcatc cggcaagtcg ctggcctatc agttgccgat cctgacggca
     3301 ttcgccaccg atccccgtgc acgggcgttg tatctgtcgc cgaccaaggc ccttggccac
     3361 gaccaactgc gcaccgccca cgcgctcacc gatgccgtca gcaagctggg caccgtcgca
     3421 ccgagcgcgt acgacggcga cagccccacc gagatacgac gattcgcccg cgagcggtcg
     3481 cgttggatct tctccaatcc cgacatgatc cacctttcga tattgcgcaa ccatgcgcgg
     3541 tgggcggtgt tcctgcggaa cctgcgctac atcgtggtcg atgaatgcca ttactaccgc
     3601 ggcgttttcg ggtccaatgt ggcgatggtg ctgcgtcgtc tgctgcggct gtgcacccgc
     3661 tattcggcgg ccccgacggt gatcttcgcc agtgcgacga ccgcctcccc tgcggccacc
     3721 gcatcggagt tgatcggcca gacggtgctc gaagtgacag aagacggttc accgcagggc
     3781 gcgcgcaccg tcgccttgtg ggagcccgcg ctgcgcaccg acatggtcgg cgagaacggc
     3841 gcgccggtgc ggcgttctgc cggcgcggag gcggcgcggg taatggccga cctggttgcc
     3901 gaaggcgccc gcacactgac attcgtccga tcccgccgcg gtgccgagct cacggcgctg
     3961 ggtgcccgtt cgcggctgga ggaggtcgct ccagacttgg cgagcaaagt cgcgtcgtat
     4021 cgtgccggct acctcgccga ggaccgtcgt gaactggagc gcgcgctggc ccacggcgac
     4081 ctaagaggcc tggccagtac caatgcactc gaactcggag tcgacatcgc gggactggac
     4141 gctgtcgtgc tcgcaggctt ccccgggacc gtgacctcgt tttggcagca ggccggacga
     4201 tccggcagac gggggcaggg cgcgctgatt ctcctgatag cgcgcgacga cccactggac
     4261 acctatctgg tgcaccatcc ggaggcgctg ttggacaagc caattgaacg ggtggtgatc
     4321 gaccccggca atccgtatgt gttgggtccg caattgctct gtgcggcaac ggagctgcca
     4381 ttgaccgacg cggaggtacg cgcatggggt gccgaggccg tcgccgagac cctggtcgac
     4441 gacgggctgt tgcgccgacg tccgagtggt tacttcccca gcccaggcgt cgacccccat
     4501 cccgcggtcg acatccgcgg ctcgtccggg ggccagatcg cgattctgga aac
//
LOCUS       DASYLX010000265         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_230344,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000265 DASYLX010000000
VERSION     DASYLX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230344"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VGP27_06500"
     CDS             complement(<1..1151)
                     /locus_tag="VGP27_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV7851000.1"
                     /translation="MSSLRRGDRGGAVTEIRTALAALGLVDSPDADLSTGRHVAVDFF
                     DLELDEAVRAFQQHRGLLVDGIVGEATYRALREASYRLGARILSHQFGAPMYGDDVAT
                     LQARLQDLGFYTGLVDGHFGLQTHNALMSYQREYGMSPDGICGPETLRSLYFLGSRVT
                     GGSIHAIREEEVVRRSGPRLSGKRIIIDPGRGGDDHGQITQSSSGPISEADILWDLAS
                     RLEGRMTAIGMETFLSRPPNRSPSDAERAMTANTVGADLMISLRCAAQASPSANGVAS
                     FHFGNSHGSVSTIGRNLADFIQREVVARTGLRDCRTHGRTWDLLRLTRMPTVQVDVGY
                     ITNPRDRGMLVANQTRDSIAEGILAAVKRLYLLGKNDRPTGTFTFAELLA"
     gene            complement(1286..1627)
                     /gene="trxA"
                     /locus_tag="VGP27_06505"
     CDS             complement(1286..1627)
                     /gene="trxA"
                     /locus_tag="VGP27_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7851001.1"
                     /translation="MTGTEKTSKTVNVTDDSFSEDVLSSSTPVLVDFWATWCGPCKMV
                     APVLEEIAGEKVGSLTIAKLDVDANPTTARDFQVVSIPTMILFKGGEPVKRIVGAKGK
                     AALLRELSDIV"
     gene            complement(1624..2607)
                     /gene="trxB"
                     /locus_tag="VGP27_06510"
     CDS             complement(1624..2607)
                     /gene="trxB"
                     /locus_tag="VGP27_06510"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV7851002.1"
                     /translation="MTSQSSVHDVIIIGSGPAGYTAAIYAARAQLTPLVFEGSQFGGA
                     LMTTTEVENYPGFRDGITGPELMDQMREQALRFGADLRMEDVDEVSLKGPVKSVTVGD
                     EKYYARAVILAMGAAARHLGVPGEDRLLGMGVSTCATCDGFFFRDQDIAVVGGGDSAM
                     EEATFLTRFARSVTLIHRRDEFRASRIMLERAKANEKIRFMTNTQVLEVQGDNRVTGV
                     RLLNSVTGEESSLAVTGVFVAIGHDPRSDLVRGQVDLDPAGYVLVRDRTTSTSVEGVF
                     AAGDLVDHIYRQAVTAAGTGCSASIDAERWLAETNTGTTQTIDTDLIGAQP"
     gene            complement(2681..3040)
                     /locus_tag="VGP27_06515"
     CDS             complement(2681..3040)
                     /locus_tag="VGP27_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851003.1"
                     /translation="MRGGESGLPGDRVRQALVHLGTDSASAPDVPASVTARISAALRA
                     APSAPSHTTARPRSSRSRTILLLVGIAAAAAGIAVAVALLLHTPANPRFPSGPTAEKI
                     TVSTTPADTPKAVVTPP"
     gene            complement(3055..>3242)
                     /locus_tag="VGP27_06520"
     CDS             complement(3055..>3242)
                     /locus_tag="VGP27_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV7851004.1"
                     /translation="QRAAVVAVDMQGYSVAETARMLGIAEGTVKSRCSRARAKLADAL
                     GNFDTRVDVPDARGALR"
BASE COUNT          540 a         1053 c         1103 g          546 t
ORIGIN      
        1 gcgagcagct cggcgaatgt gaaggttccg gtggggcggt cgttcttgcc caacaggtag
       61 agccgtttga cggcggcgag gatgccttcg gcgatcgagt cccgggtctg gttggcgacc
      121 aacatgccgc ggtcccgagg gttggtgatg tagccgacgt ccacctgaac cgtcggcatc
      181 cgcgtcagac ggagcagatc ccacgtccgc ccatgggtgc ggcagtcacg taaaccagtg
      241 cgcgccacga cttcacgctg tatgaagtca gcgaggttcc ggccgatcgt ggacaccgat
      301 ccgtgcgagt ttccgaagtg gaacgacgcg acgccgttcg cggacgggct tgcctgggcg
      361 gcgcaccgca ggctgatcat caggtcggcg ccgacggtgt tggcggtcat ggcccgttcg
      421 gcgtccgacg ggctgcggtt gggcggccgg gacaggaagg tctccatgcc gatggcggtc
      481 atccggccct cgagccggct cgccagatcc cagaggatgt cggcttcgct gatgggaccc
      541 gaggagcttt gggtgatctg cccgtggtcg tcgccaccgc ggcccggatc gatgatgatg
      601 cgcttgccgg acagccgtgg tcccgacctg cggaccactt cctcctcgcg gatggcgtgg
      661 atggagccgc cggtgacgcg tgagcccaga aagtacaagg agcgcaacgt ttccgggccg
      721 cagatgccgt cgggggacat tccgtactca cgctgatacg acatcagcgc gttgtgggtc
      781 tgcagcccga aatggccgtc caccagcccg gtgtagaaac ccaggtcctg aaggcgcgcc
      841 tgcagggtgg cgacgtcgtc gccgtacata ggcgcgccga attggtggct gaggatgcgc
      901 gcgccgagtc gataggacgc ttccctgagt gcgcgatacg tcgcttcgcc gacgatgccg
      961 tcgacaagca ggccacgatg ctgctggaaa gcgcgaaccg cctcgtcgag ttcgaggtcg
     1021 aagaagtcga ccgcgacgtg tctaccggtg ctcaggtcgg cgtcggggct gtccaccagc
     1081 ccgagcgccg cgagcgcggt ccggatctcg gtgaccgcac ccccgcggtc acctcgacgc
     1141 agactcgaca tgtatgggcc ctttcgataa cgcttatcgg cggcggtcca gcggtgacga
     1201 ttgcaggaga ggctcagcgg aattatctca gaagcaggcc cgattcaaga aaacgccagg
     1261 cgagtcgccg cggggtcgcc agacctcaga cgatgtcgga gagctcgcga agcagcgccg
     1321 ccttgccctt ggcgccgaca atccgcttca ccggctcgcc gcccttgaac agaatcatgg
     1381 tcggaatcga tacgacctga aaatcgcgcg cggtggtggg gttggcgtcg acgtcgagct
     1441 tggcgatcgt gagcgaaccg accttctcgc ccgcgatctc ctcgagcacc ggggcgacca
     1501 tcttgcacgg accgcaccag gtggcccaga agtccaccag aacgggagtg ctgctcgaca
     1561 gtacgtcttc cgagaacgag tcatcggtga cgttcaccgt cttgctggtc ttctcagtgc
     1621 cggtcatggc tgtgctccaa tcaggtcggt gtctatggtt tgcgttgtcc ccgtattggt
     1681 ttcggcgagc caccgttcgg cgtcgatcga ggccgaacag ccggttcccg ccgcggtgac
     1741 cgcctgccgg tagatgtggt cgacaaggtc gcccgccgcg aagacacctt cgaccgacgt
     1801 gctcgtggtg cggtcacgca ccagcacgta cccggccgga tcgaggtcga cctggccgcg
     1861 gaccaaatct gaccgcgggt cgtgaccgat cgcgacgaac acaccggtca cggccaggct
     1921 ggattcctcg ccggtcacgc tgttgagcag acgcaccccg gtcacccggt tatccccctg
     1981 gacctccagc acctgcgtgt tggtcatgaa gcggatcttc tcgttggcct tggcgcgttc
     2041 cagcatgatg cgcgatgcgc ggaactcgtc gcgccggtgg atcaacgtca cgctgcgggc
     2101 gaaccgggtc aggaaggtgg cctcctccat tgcggagtcg ccaccgccga ccacggcgat
     2161 gtcctggtca cggaagaaga acccgtcgca ggtcgcacac gtgctcacac ccatgcccag
     2221 caacctgtcc tcaccgggaa cgcccaggtg gcgggccgct gcgcccatcg cgagaatcac
     2281 ggcgcgggcg tagtacttct cgtcaccgac ggtgacggac ttcaccggcc ccttgagcga
     2341 gacctcgtcg acgtcttcca tccggagatc ggcgccgaac cgcagcgcct gctcgcgcat
     2401 ctgatccatc aactcgggtc cggtgatgcc gtcgcggaag cctgggtagt tctccacttc
     2461 ggtcgtggtc atcagcgcgc cgccgaactg cgagccctcg aacaccaggg gtgtgagctg
     2521 ggcccgggcc gcgtagatcg cagccgtgta gccggccggt cctgagccga tgatgatgac
     2581 gtcgtgcaca gaagattgag aagtcatgcg agcctttctg cctgcacgga tacaaacacc
     2641 agggtaagcc ggggtgttcc cccatcggtg gtgggctgcg ttacgggggc gtgaccacgg
     2701 ctttaggcgt gtcggcgggt gtcgtcgaga cggtgatctt ctccgccgtc ggtccggacg
     2761 ggaaacgcgg gttcgcaggg gtgtgcagca gcaacgccac ggcgaccgcg atcccggctg
     2821 ccgcggcggc gatgccgaca agcagcagaa tggtgcgcga ccggctcgac cgcggtcggg
     2881 cggtggtgtg cgacggcgcg gacggtgccg cgcgcagcgc cgcgctgatg cgggccgtca
     2941 cggaagcggg aacgtcaggt gccgaggccg agtcggtccc aagatggacg agtgcctgac
     3001 gcacccggtc cccgggaagc cccgattcgc cgcctcgcat ccgtcaatag tgactcaccg
     3061 cagggcgccc cgggcgtctg gcacgtccac gcgagtatcg aaattgccaa gcgcgtcggc
     3121 gagtttggcg cgggcacgcg aacagcggct cttcacggtg ccctccgcga tgcccagcat
     3181 ccgggcggtc tcggccaccg agtaaccctg catgtcgacc gcgaccaccg cggcacgctg
     3241 ct
//
LOCUS       DASYLX010000266         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2972477,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000266 DASYLX010000000
VERSION     DASYLX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972477"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VGP27_06525"
     CDS             complement(<1..247)
                     /locus_tag="VGP27_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV7851005.1"
                     /translation="MTNEPAIDSDGGLVRISSRPVIRLRLDIAYDGTEFAGWAVQAGQ
                     RTLAGVLDEALTTVFRTPVQLRAAGRTDTGVHATAQVA"
     gene            complement(252..881)
                     /gene="rplQ"
                     /locus_tag="VGP27_06530"
     CDS             complement(252..881)
                     /gene="rplQ"
                     /locus_tag="VGP27_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV7851006.1"
                     /translation="MPKPTKGRRLGGSSSHQKALLANLATSLFEHGRIKTTEPKARAL
                     RPYAEKLITHAKKGTLHNRREVLKKIRDKDVVHTLFAEIGPFYADRAGGYTRIIKVEA
                     RQGDNAPMAVIELVREKTVTSEANRARRTGAAKKAAPVAAAAAPQAAVEPEEAVGPDA
                     EESVEEAKASDETSVEEAKAEDEAIADAQTAEATDADADEADSEDADKS"
     gene            complement(922..>1600)
                     /locus_tag="VGP27_06535"
     CDS             complement(922..>1600)
                     /locus_tag="VGP27_06535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV7851007.1"
                     /translation="ATLNDKGKLEVELVVERGRGYVPAVQNKASGAEIGRIPVDSIYS
                     PVLKVTYKVEATRVEQRTDFDKLILDVETKSSITARDALASAGKTLVELFGLARELNV
                     EAEGIEIGPSPAEADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVARTESDLLD
                     IRNFGQKSIDEVKIKLHQLGLSLKDSPATFDPSEVAGYDVATGTWNSDAGYELDNDQD
                     FAETEQL"
BASE COUNT          230 a          536 c          521 g          313 t
ORIGIN      
        1 gggccacctg cgcggtcgcg tgcacgccgg tgtcggtacg tccggctgcg cgcagctgca
       61 caggggtgcg gaacaccgtc gtcagcgcct cgtcgaggac accggcaagg gtgcgctgtc
      121 ccgcctgcac cgcccagccg gcgaactctg ttccgtcgta agcgatgtcg aggcgaagac
      181 gaatcaccgg acgcgaggag atacgaacaa gcccgccatc ggaatcgatg gcgggctcat
      241 tcgtcatgtc actaggactt gtcggcatcc tcggagtcag cctcgtcagc gtcagcgtcg
      301 gtcgcctctg ccgtctgagc gtcggcgatc gcctcgtcct cggccttcgc ctcctcgacc
      361 gaggtctcgt cggaagcctt cgcctcctcg accgattcct cggcgtccgg tccgacagct
      421 tcctccggct caaccgccgc ttgcggcgcc gcggcggccg cgacgggcgc agccttcttc
      481 gccgcacccg tgcgacgcgc acggttggcc tcggacgtca ccgtcttctc ccgcaccagc
      541 tcgatgacgg ccatcggagc attgtcgccc tgacgtgcct ccaccttgat gatgcgggtg
      601 tagccgccgg cgcggtcggc atagaacggc ccgatctcgg cgaacagggt gtgcacgaca
      661 tccttgtccc ggatcttctt gagcacctca cgccggttgt gcagcgtgcc cttcttggcg
      721 tgggtgatca acttctccgc gtacggccgc agcgcccgcg ccttgggctc ggtggtcttg
      781 atcctgccgt gctcgaaaag cgatgtggcc aggttggcca gcagcgcctt ctggtgcgag
      841 gacgacccgc cgaggcgacg gcccttggtg ggcttgggca ttggtgactc cttgtattac
      901 ggccgagttg gctaactcgg tctagagctg ttcggtttcg gcgaagtcct gatcgttgtc
      961 cagctcgtag ccggcgtcgc tgttccacgt tccagtggcg acgtcgtagc ctgcgacctc
     1021 ggacggatcg aacgtggccg ggctgtcctt cagcgagaga ccgagctggt gcagcttgat
     1081 cttcacctca tcgatcgact tctggccgaa gttgcggatg tccagcagat cggactccgt
     1141 gcgggcgacg agctcgccca ccgtgtgcac accctcgcgc ttgaggcagt tgtacgaccg
     1201 gaccgtgagg tccaggtcgt cgatcggcag tgcgaacgat gcgatgtgat cggcctcggc
     1261 gggcgacggg ccgatctcga tgccctccgc ctcgacgttg agttcgcgtg ccagaccaaa
     1321 caattcgacc agcgtcttgc ccgccgacgc cagcgcgtca cgagcggtga tcgagctctt
     1381 ggtctcgacg tccaggatca gcttgtcgaa gtcggtgcgc tgctcgacgc gggtggcctc
     1441 caccttgtag gtgaccttaa gcaccggcga gtagatggaa tcgaccggga tgcggccgat
     1501 ttcggcgccc gacgccttgt tctgcacggc cgggacgtag ccgcggccac gctcgacgac
     1561 gagctcgacc tccagcttgc ccttgtcgtt cagggtggcg
//
LOCUS       DASYLX010000267         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3521205,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000267 DASYLX010000000
VERSION     DASYLX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521205"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGP27_06540"
     CDS             <1..638
                     /locus_tag="VGP27_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7851008.1"
                     /translation="SLGPLAKAAITKTIPLTIISFVIGLVIALVVALARLSSNVVVSN
                     IARFYISVIRGTPLLVQLFIVFFALPELGIRIDPFPAAVIAFSLNVGGYAAEIIRGAI
                     QSIPKGQWEAAETIGLDYVDSLRRIILPQATRVAVPPLSNTLISLVKDTSLASTILVT
                     ELLRQAQIIAAPTFEFFALYGTAAVYYWVICLVLSFGQSRLERRLERYVAR"
     gene            635..1408
                     /locus_tag="VGP27_06545"
     CDS             635..1408
                     /locus_tag="VGP27_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV7851009.1"
                     /translation="MTEPRENRIVAEDVQKAFGDNQVLKGVSFTVARGSATAIIGPSG
                     SGKTTLLRALNALDVPDAGVIRVGGDDGVEIDFSKPVAKDQLRRYRAQSGFVFQSHNL
                     FPHKTVLENVIEGPVIAQKRPKDQAVAEAVELLQQVGLAEKRDQYPFQLSGGQQQRVG
                     IARALAIKPKVVLFDEPTSALDPELVGEVLSVIRDLAVEGWTLVIVTHEIQFARQVSD
                     QVLFTDGGVILEKGPPAEVIGNPKEERTRQFLDRILNPL"
BASE COUNT          290 a          525 c          549 g          290 t
ORIGIN      
        1 acagcctcgg gccactggcc aaggccgcga tcaccaagac gatcccgctg acgatcatca
       61 gtttcgtcat cgggctggtg atcgcgctcg tagtagcgct ggcccggctg tcgtcgaatg
      121 tggtcgtcag caacatcgcc cgcttctaca tatcggtgat ccggggcacg ccgctgctcg
      181 ttcagttgtt catcgtgttc ttcgcactgc cggagctcgg gatcagaatc gatcccttcc
      241 ccgcggcggt catcgcgttc tcgctcaacg tcggcggcta cgccgcggag attattcgcg
      301 gcgctatcca aagcattccc aagggacagt gggaggccgc cgagacgatc ggcctggact
      361 acgtcgactc gctgaggcgc atcatcctgc cgcaggccac cagggtcgcc gtgccgccgc
      421 tgtcgaacac gctgatctcg ctggttaagg acacgtcgct ggcatcaacg attctggtca
      481 ccgagttgct gcggcaagca cagatcatcg cggcgccgac gttcgagttc ttcgctctgt
      541 acggcaccgc cgcggtttac tactgggtca tctgcctggt gctctcgttc ggtcagagcc
      601 gtcttgaacg tcggctggaa aggtatgtgg cgcgatgacg gagcctcgtg agaacaggat
      661 cgtcgccgag gatgtgcaga aggccttcgg cgacaaccaa gtgctgaaag gcgtctcgtt
      721 caccgtcgcg cgcggatcgg cgacggcgat catcggcccg tcgggttcgg gcaagacgac
      781 gctgctgcgc gcgctcaacg cactcgacgt gccggatgcc ggagtcatcc gggtcggcgg
      841 ggacgacggg gtcgagatcg acttctccaa gccggtcgcc aaggaccagc tgcggcggta
      901 ccgggcgcaa agcggctttg tgttccagtc ccacaacctg tttccgcaca agacggtctt
      961 ggagaacgtc atcgagggac cggtgatcgc gcagaagcgg ccgaaggacc aggcggtggc
     1021 cgaggctgtc gaactgctgc aacaggtggg gctggccgag aagcgggacc agtacccgtt
     1081 ccagctgtcc ggtggccagc agcagcgggt cggcatcgcg cgggcgctgg cgatcaagcc
     1141 gaaggtggtg ttgttcgacg agccgacctc ggcgctggac ccggagttgg tcggcgaggt
     1201 gctgtcggtc atcagggacc tcgccgtcga gggctggacc ctggtcatcg tcacccacga
     1261 aatccagttc gccaggcagg tttccgacca ggtgctgttc accgacggag gggtcatcct
     1321 cgagaagggg ccgcccgccg aggtgatcgg caatccgaaa gaggagcgga cgcgccagtt
     1381 cctcgaccga atcctcaacc cgttgtaaaa gccggaacct tcccgcttcg gcgctacctc
     1441 ccgctccgcg acggtgcgtg tctgttgcac gacacgcgca aaagcgtggc attctgcgca
     1501 cgctcggcgc cggccactgc agcgccgtgc gcaaactacg gtaaggacct cggcgtgtcg
     1561 gctgcagaca cgctcccccg cttgaagcgg gaggtacccc ggccggcggg agaactgagg
     1621 ccacgatggt gcaaccgacc aactgggacg ccga
//
LOCUS       DASYLX010000268         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3521225,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000268 DASYLX010000000
VERSION     DASYLX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521225"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /gene="gcl"
                     /locus_tag="VGP27_06550"
     CDS             <1..1480
                     /gene="gcl"
                     /locus_tag="VGP27_06550"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HEV7851010.1"
                     /translation="VAKLHKEDFQAVDITAIAAPVTKMAMTVLEPGQVPGAFAQAFHL
                     MRSGRPGPVLIDLPIDVQLAEIDFDPDSYQPLPVYKPAATRGQAAKAIDMLASAERPL
                     IVAGGGIINADASDLLVELAEVLGVPVVPTLMGWGTIPDDHRLAAGMVGLQTAHRYGN
                     ATMLESDFVLGIGNRWANRHTGGLDTYRSGRRFVHVDIEPTQIGRVFSPDFGIVSDAK
                     AALEQFVAVAKERRAADTLPDWRGWVDDCQLRKSTMHRKTHFDDVPIKPQRVYEEMNR
                     VFGRDARYVSAIGLSQIAGGQFLQVFKPRNWINCGQAGPLGWTVPAALGVAAADPDAT
                     VVGLSGDYDFQFLIEELAVGAQFNLPYVHVLVNNSYLGLIRQAQRNFDMDYCVSLAFD
                     NINSQETDGDVPKGYGVDHLRVVEGLGCKAIQVVEPQDIGPSLARAQQLAREHKVPVV
                     VEVFLEKITNIAMGTEIDNVVEFNDLAESLADAPTAAALLVD"
     gene            complement(1654..>2376)
                     /locus_tag="VGP27_06555"
     CDS             complement(1654..>2376)
                     /locus_tag="VGP27_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7851011.1"
                     /translation="AFELLEIMASAGPSIAFGELAARAELPQPTIHRLVRTLLNMGYV
                     RQLPNRHYALGPKLIRLGESAAQLIGAWSRPHLAELVERTGESANMAVLDNDMAVYVA
                     QVPSPHSMRMFTEVGRRVHLHCTGVGKALLMQLPDEAVLAVLKRAGMPASSENTHTTP
                     DALMRDLELSRSRGYAVDEGEQEIGVRCFAVPVPDAPTMTAVSISGPASRLTLKSATK
                     VAPLLKRVARDLASEFDKEAAV"
BASE COUNT          443 a          811 c          724 g          398 t
ORIGIN      
        1 cgtggccaaa ctccacaaag aggacttcca agcggtcgac atcaccgcca tcgccgcacc
       61 ggttaccaag atggcgatga cggttctcga accgggccag gttcccggtg cattcgctca
      121 ggcgtttcac ctgatgcgtt ccgggcggcc ggggccggtg ctcatcgacc tgcccatcga
      181 cgtccaactg gccgagatcg atttcgaccc ggattcctac cagccgctgc ccgtgtacaa
      241 gccggcggcg acgcgtggcc aggccgccaa ggcgattgac atgctggcga gcgccgaacg
      301 accactcatc gtcgcgggcg gaggcatcat caacgccgat gcctccgatc tgctggtgga
      361 gctggccgag gtcctcggtg tcccggtggt gccgacgctg atgggctggg gcacgatccc
      421 cgacgatcac cgcctggcgg ccggcatggt cggactgcag accgcccacc gctacggcaa
      481 cgcgaccatg ctcgaatccg acttcgttct gggaatcggc aacaggtggg ccaaccgaca
      541 caccggcggg ctcgatacct atcgcagcgg acggcgtttc gttcacgtcg acatcgaacc
      601 cacccagatc ggccgcgtct tctcccccga cttcggaatc gtctcggacg cgaaggccgc
      661 actcgagcag tttgtcgccg tggccaagga gagacgcgca gccgacacgc tgcccgattg
      721 gcgcggttgg gtcgacgact gccagttgcg caagtcgacg atgcatcgca agacgcactt
      781 cgacgacgtt ccgatcaaac cccagcgcgt ctacgaggaa atgaaccggg tcttcggccg
      841 cgatgcccgc tacgtcagcg cgatcgggct gtcacagatc gccggcggac agttcctgca
      901 ggtgttcaag ccacgcaact ggatcaactg cggccaagcc ggaccgctcg gatggaccgt
      961 gcctgccgcc cttggtgtcg ccgccgccga ccccgacgcc accgtggtgg ggctttcggg
     1021 cgattacgac ttccagttcc tgatcgagga actcgcggtc ggggcgcagt tcaacctgcc
     1081 ctacgtgcat gtcctggtga acaactccta cctgggactg atccgccagg cgcagcgcaa
     1141 cttcgacatg gactactgcg tgtcattggc gttcgacaac atcaactccc aggagacgga
     1201 cggcgatgtc cccaagggat acggcgttga tcatctgcga gtcgttgagg gcctgggctg
     1261 caaggcaatt caggtggtcg aaccgcagga catcgggcca tcgctggcac gggcgcaaca
     1321 acttgcccgc gagcacaagg tgcccgtggt ggtcgaggtc ttcctggaga agatcaccaa
     1381 catcgcgatg ggcaccgaga tcgacaacgt cgtcgagttc aacgatctcg ccgaatcgct
     1441 cgcagacgca ccaacggctg ccgcactgct cgtcgactaa cgctggccga agtctgcgag
     1501 acagctcggc gtgcccgact ctgagcgatt gcagaaccac ttccaagccc ggcgacgatc
     1561 ggacgtcgag cgctgcgcgg cttcacccat tgactctgcg tacatggcga caaagtgcgc
     1621 gagagaccca agttctccgc gcagagtcaa cgattaaacc gccgcctctt tgtcgaattc
     1681 cgacgcgaga tcgcgagcca cccgtttgag cagcggcgcg accttcgtcg ccgacttcaa
     1741 ggtgagacgc gacgccggac cggagatcga gaccgcggtc attgtgggtg cgtccggcac
     1801 cggcaccgcg aagcaccgca caccgatctc ctgctcgcct tcgtccaccg catagccgcg
     1861 cgaccggctg agctcgagat cccgcatcaa cgcatccgga gtggtgtgtg tgttctcgct
     1921 ggaggccggc atcccggcgc gcttcagcac cgcgagcacc gcctcgtcgg gaagctgcat
     1981 cagcagcgcc ttgcccaccc cggtgcaatg caagtgcacg cgacggccca cttccgtgaa
     2041 cattcgcatc gagtgcggcg acgggacttg agcaacatag accgccatgt cgttatccag
     2101 caccgccatg ttggcgctct cgcccgtccg ctccaccagc tcagcaaggt gtggccgcga
     2161 ccacgcgccg atgagttggg ctgcgctctc ccccagccga atcaacttgg gccccagcgc
     2221 atagtgccga ttgggcagct gcctgacgta ccccatgttc agcagcgtcc gcaccagacg
     2281 atgaatggtt ggttgcggca gttcggcacg agccgccaac tccccgaacg cgattgacgg
     2341 gcccgcgctc gccatgatct cgagcagctc gaacgc
//
LOCUS       DASYLX010000269         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2204820,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000269 DASYLX010000000
VERSION     DASYLX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204820"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGP27_06560"
     CDS             complement(<1..358)
                     /locus_tag="VGP27_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7851012.1"
                     /translation="MPEIPEQYLLSPYAPQPRTLIDILYDTAARYPDAPAIDDGSVQL
                     TYSELIVDIEDSVAWLAARGIGRGDRVGVRMPSGSYALYVAILATLATGAAYVPADAD
                     DPDERAALVFGEANVVG"
     gene            complement(377..1006)
                     /locus_tag="VGP27_06565"
     CDS             complement(377..1006)
                     /locus_tag="VGP27_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV7851013.1"
                     /translation="MADTELPGVFGDLQPLLEHYGYLAVGGFVLLEDFGVPVPGETIL
                     IAAAVFAGSGHMNIALVILVAVVGAIIGDNIGFAVGHFGGRPLVHRFGRYIFLTPARL
                     DHAEEYFNKHGGKIVTVARFIEGLRQLNGLLAGIIGMHWAKFLVFNALGAVLWVCTFA
                     AVGYLAGENIVEIYAAFERYKWYVIAAIVVIAAILITRRIRRRRAAAGA"
     gene            1096..1491
                     /locus_tag="VGP27_06570"
     CDS             1096..1491
                     /locus_tag="VGP27_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851014.1"
                     /translation="MRLFVADASGDNWNELTSGEATAVRLAAPDLQQARRARGRITGD
                     VAVILDITVAVAADFRSARDAMPIIDERTLHYAGTIDGLAGLVADIFMADVADGVTFI
                     PASPEQDMRELADAAVDRIARRLPLSGAA"
     gene            complement(1496..2188)
                     /locus_tag="VGP27_06575"
     CDS             complement(1496..2188)
                     /locus_tag="VGP27_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851015.1"
                     /translation="MAPLFETAGHFWWLIFPLAGMVGGGVRAIAAANERRAERRLERY
                     RIKQQTKIAVAEAAGRTRAGQVKNRRECKRVIEQHGQTDARWLEYEVDVAKLLDFPLM
                     TDMRNELTIAFHKARRRADLLRPQNAEDLDGDREAQLEYRNAVGDYVTAFDVAEAEAI
                     RLRRSTFSEEDQQRIARAQSLLRLAEDDGATPQERHNAYTRARVELDGILALPAVTRA
                     SIERKLAGELEA"
BASE COUNT          352 a          773 c          768 g          379 t
ORIGIN      
        1 cccccacgac gttggcctcc ccgaacacca gcgcggcccg ctcgtcgggg tcgtcggcgt
       61 ccgccggaac gtaggcggcg ccggtggcga gcgtcgcgag gatcgccacg tagagggcgt
      121 aactgcccga cggcatgcgg acgccgacgc ggtcaccgcg tccgatgccg cgcgccgcca
      181 gccacgcgac actgtcctcg atgtcgacga tcagctcgct gtaggtcagc tgcacggagc
      241 cgtcgtcgat cgcgggcgca tcgggatagc gtgccgcggt gtcgtacagg atgtcgatca
      301 gcgtccgcgg ctgcggcgcg tacggcgaca gcaggtactg ctcaggaatc tccggcatcg
      361 ccacgcatac aacgtactaa gcgcctgccg ccgcgcgtct gcgcctgatg cggcgtgtga
      421 tcagaatggc cgcgatgaca acgatcgcgg cgatgacgta ccacttgtaa cgctcgaacg
      481 cggcatagat ctcaacgatg ttctcaccgg cgaggtagcc gacggcggcg aatgtgcaca
      541 cccacagcac tgcgccgagc gcgttgaaga ccaggaattt cgcccagtgc atgccgatga
      601 tcccggccag cagcccgttg agctgccgca gcccctcgat gaaccgcgcg acggtgacga
      661 ttttgccgcc atgcttgttg aagtactcct cggcatggtc gagtcgcgca ggcgtcaaga
      721 agatgtaccg cccgaatcga tggacgagcg ggcggccgcc gaagtggccg acggcgaatc
      781 cgatgttgtc gccgatgatc gcgccgacca ccgccaccag gatcaccagc gcgatgttca
      841 tgtggcccga gcccgcgaag accgccgcgg cgatcagtat ggtttcgccg ggtaccggca
      901 cgccgaaatc ctcgaggagc acgaagccgc cgacggccag atagccgtag tgttcgagca
      961 gcggctgcaa gtccccgaac acaccgggta gctcggtgtc ggccatgtct caaattctgg
     1021 ttgagcgcaa ccgttgtcgt gcagcgatcg cgtgcctagc gtgtcggcgt ggcaagcgta
     1081 cgcggtgatc gggggttgcg gctcttcgtg gccgacgcat ccggcgacaa ctggaacgaa
     1141 ctcacctcgg gtgaggcaac ggcggtgcgg ctggcggcac ccgacctgca gcaggctcgg
     1201 cgagctcgcg gccggatcac cggcgacgtc gcggtgatcc tcgacatcac cgttgcggtc
     1261 gccgccgact tccggtctgc gcgagacgcg atgccgatca tcgacgagcg caccctgcac
     1321 tacgcgggaa ccatcgacgg gctggccgga ctcgtcgccg acatcttcat ggccgatgtg
     1381 gccgacggcg tgacgttcat cccggcgtcg ccggagcagg acatgcgcga gctggccgac
     1441 gcggcggtgg accggatcgc acgccgcctg ccgctctccg gcgcggcctg accggctacg
     1501 cctcgagctc tcctgcgagc ttgcgttcga tgctggccct cgtgacggcg ggcagcgcga
     1561 gtatcccgtc gagttcgacc cttgcccgcg tgtaggcgtt gtggcgttcc tggggggtgg
     1621 cgccgtcgtc ttcggcaagc cgcagcaggc tctgggcacg tgcgatgcgc tgctggtcct
     1681 cttcggagaa ggtgctgcga cgcagccgga tcgcctcggc ctcggcgacg tcgaacgccg
     1741 tcacatagtc accgacggcg ttgcggtact ccagctgagc ctcgcggtcg ccgtcgaggt
     1801 cctcggcgtt ctgcggtcgc agcaggtccg cccgtcgccg tgccttgtgg aaggcgatcg
     1861 tcagctcatt gcgcatatcg gtcatcagcg ggaagtcgag gagcttggcg acgtcgactt
     1921 cgtactccag ccaccgcgca tcggtctggc cgtgctgctc gatcacccgc ttgcactcgc
     1981 gccggttttt cacctggccg gcgcgcgttc ggccggccgc ctccgcgacg gcgatcttgg
     2041 tctgctgttt gatccggtag cgctcgagcc gacgttcggc acgccgctcg ttggccgcgg
     2101 cgatcgcccg cactccgccg cccaccatgc cggcgagggg gaagatcagc caccagaaat
     2161 ggcccgcggt ctcgaacagg ggtgccacat tgccagcatg ccaccgtcag gacagcgcct
     2221 cgtagatcag cttggcgtac ctcgcggtgc gctcgttgcc gagcgtgccc tg
//
LOCUS       DASYLX010000270         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_339801,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000270 DASYLX010000000
VERSION     DASYLX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339801"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1205
                     /locus_tag="VGP27_06580"
     CDS             39..1205
                     /locus_tag="VGP27_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7851016.1"
                     /translation="MGLDDRDGLRMLRDAVDPRLGGEELIRQFYTKWFAIDMAVRDLF
                     PPDLVPQFKVFSQALTWLFGELIEQRAQEPVAFLAQLGRDHRKYGVLASQYFSMHEAL
                     YTTLRSRLSENWDDKLDDTARASLELFTGVMRGAAEAEQGPPFCDGTVVELKRVSRDI
                     SVVRLALDRPLAYHPGQYVTVQVPQWPRRWRYLSPAMPTDPSGAIEFHIRSVTGGMIS
                     PTVVVETEPGDRWRLSNPHGGMQIDRNGGDVLMVAGSTGLAPLRALIMDLTRWGVNPR
                     VHLFFGARYPCELYDLRTLWQIAANNPWLSVSPVSEFSQDPPWATDFPDVQPPRGLHV
                     RQTGKLPEVVTRYGNWGDRQILICGGPGMVSATKQALIAKGASAERIQHDPLAG"
     gene            1231..2133
                     /locus_tag="VGP27_06585"
     CDS             1231..2133
                     /locus_tag="VGP27_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEV7851017.1"
                     /translation="MGEFESVTLRDPSSSLSATYVPVAGMICTSLADDGVEFLGQRRG
                     LDAYVTNGKTMGIPILYPWANRLSASKYGINGAVVSLTVGTGGVRADEHGVPIHGVLA
                     AYPGWLVTAKSENALTAVLDYGGRPRLLASFPFPHVLTQQITLVDRTLTIETTVMPTT
                     AASVPLCFGYHPYFQIPDVPRAQWTLTTPNMRRLPVDNWGIPTGDTEDWNGATEPLKN
                     IAYDDGFDEVPDGAVFALQGGDHRIEVKFEKGYPAAQLFAPPGEGLIAIEPMAAPTDA
                     LSRGNYRYAAAGKPETARFSIRVT"
     gene            complement(2169..2579)
                     /locus_tag="VGP27_06590"
     CDS             complement(2169..2579)
                     /locus_tag="VGP27_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7851018.1"
                     /translation="MAPKRSDDRESARTFLISVAAELAGMHAQTLRTYDRLGLVSPQR
                     SSGGGRRYSERDVDLLREVQRLSQDEGVNLAGIKRIIELTNQVQALQSRLSEMAGELD
                     QLRAKRSRDVVVVPKSTALVVWQPGAARRRHRAE"
     gene            complement(2579..>2950)
                     /locus_tag="VGP27_06595"
     CDS             complement(2579..>2950)
                     /locus_tag="VGP27_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV7851019.1"
                     /translation="APSGDLYVTVHVKPDKVFGRDGNDLTVTVPVSFTELALGTTLSV
                     PTLEGKVGVRVPKGTSDGRILRVRGRGVPKRSGGHGDLLVTVKVAVPPNLEGEASEAL
                     ENYAKAERASGFDPRAGWAGA"
BASE COUNT          477 a         1018 c          963 g          492 t
ORIGIN      
        1 cgccgcgcat ccgctcgact cacctagact cgagcggcgt gggactcgat gaccgtgacg
       61 ggctgcggat gctgcgcgac gcggtcgacc cacgcctcgg gggcgaagaa ctgatccgcc
      121 agttctacac aaaatggttc gccatcgaca tggcggtgcg tgacctgttc ccgcccgatc
      181 tggtccccca gttcaaggtg ttcagccagg cactgacatg gctgttcggc gagctcatcg
      241 agcagcgggc ccaggagccc gtcgccttcc tggctcagct gggccgcgat catcgcaagt
      301 acggcgtgct tgcgagccag tacttcagca tgcacgaggc gctctacacg accttgcgca
      361 gtcgtctgag cgagaactgg gacgacaagc tcgacgacac cgcccgcgcg tcgctcgaac
      421 tgttcaccgg ggtgatgcgt ggcgccgccg aggccgagca gggaccgccg ttctgcgacg
      481 gcaccgtcgt cgagctgaaa agggtgtccc gcgacatctc ggtggtacgc cttgcgctcg
      541 accggccgct cgcctaccac cccggccagt acgtgactgt gcaggtcccg cagtggcctc
      601 gccgctggcg ctatctcagc ccggccatgc ccacggaccc cagcggtgcc atcgagttcc
      661 acatccgctc ggtgacgggc ggaatgatca gtcccacggt cgtcgtcgaa accgaacccg
      721 gtgaccggtg gcggctgtcg aatccgcacg gcggcatgca gatcgaccgc aatggcggcg
      781 atgtgctgat ggtcgcgggc agtaccggcc ttgcgccgct gcgggcgctc atcatggacc
      841 tcacccggtg gggcgtgaac ccgagggtgc acctgttctt cggcgcgcgc tatccgtgtg
      901 agctgtacga cctgcggacg ctgtggcaga tcgccgcgaa caatccctgg ctctcggtgt
      961 ccccggtgtc ggagttctcg caggatccgc cgtgggccac cgacttccct gacgtgcagc
     1021 cgccgcgcgg cctccatgtg cgccagaccg gcaagctgcc ggaggtggtg accaggtacg
     1081 gcaactgggg cgaccggcag atcctgatct gcggcggccc aggcatggtg tccgccacga
     1141 aacaagcgct gatcgccaaa ggtgcttcgg ccgaacgcat tcagcatgat ccgctggccg
     1201 gctgaagtgg cgcgtggcag gatcgcgacc atgggtgagt tcgagtccgt aacgcttcgc
     1261 gacccgtcgt cgtcgctgtc ggcgacctac gtaccggtgg caggaatgat ctgcacctcg
     1321 ctcgccgatg acggagtcga gttcctgggc cagcgccgcg ggctggacgc ctatgtgacg
     1381 aacggcaaga ccatgggtat cccgatcctg tatccgtggg caaatcgcct gagcgccagc
     1441 aagtatggca tcaacggcgc cgttgtctcg ctgaccgtcg gcaccggcgg cgttcgcgcc
     1501 gacgaacacg gtgttcccat ccacggtgta cttgcggcct atcccggctg gctggtcacc
     1561 gccaagtcgg agaacgcctt gacggccgtc ctggactacg gaggcaggcc gcgcctgctg
     1621 gcgtcgttcc cgttcccgca cgtgctcact cagcagatca cgctggtcga ccggaccctc
     1681 accatcgaga cgaccgtgat gccgacgacg gcggcgtccg tgccgctgtg tttcggctat
     1741 cacccgtact ttcagattcc ggatgtgccg cgcgcgcagt ggactctcac cacgccgaac
     1801 atgcggcgcc tgcccgtgga caactggggc atcccgaccg gcgataccga ggactggaac
     1861 ggggccaccg agccgctgaa gaacatcgcg tacgacgacg gcttcgacga ggtgcccgac
     1921 ggcgccgtct tcgccctgca aggcggcgac cacaggatcg aggtgaagtt cgaaaagggt
     1981 tacccggcag cgcagttgtt cgcgccgccg ggcgagggac tcatcgccat cgaaccgatg
     2041 gctgcgccga cggacgctct cagtcgcggg aattaccgct acgcggccgc aggaaagccg
     2101 gagacggccc gcttctcgat cagggtgacg tagcgcttct cactcggctc gatgtcgccg
     2161 ccgagcggct actcggctcg atgtcgccgc cgagcggctc cgggctgcca gaccaccagc
     2221 gccgtgctct tcggcacgac gacgacgtcg cgactccgct tggcgcgcaa ctggtccagc
     2281 tccccggcca tctccgagag gcgcgactgc agcgcctgga cctgattggt gagttcgatg
     2341 atccgcttga tgcctgccag attgacgccc tcgtcctggg acagccgctg cacctcgcga
     2401 agcaggtcga cgtcacgctc cgaatagcga cgcccgccgc ccgagctgcg ttgcgggctc
     2461 accagcccga gccggtcata cgtgcgcagg gtctgcgcgt gcatgccagc cagttcggcg
     2521 gccaccgaga tcagaaacgt tcttgccgac tcacggtcgt cggaacgctt cggggccatc
     2581 acgcaccagc ccatccggcc cgcggatcga atccgctggc ccgctcggcc ttcgcgtagt
     2641 tctcgagcgc ttcggacgcc tcgccttcga ggttgggcgg caccgcgacc ttcacggtga
     2701 ccaacaggtc gccgtgtccg ccggaccgct tgggcacgcc tcgaccgcgc acccgcagga
     2761 tgcggccgtc cgaggtgccc ttgggcaccc gcacaccgac cttgccttcc agcgtcggga
     2821 ccgaaagcgt tgtgccaagg gcgagttcgg tgaagctgac cggaaccgtg acggtgaggt
     2881 cgttgccgtc acgaccgaac accttgtccg gcttcacatg tacggtcacg tacagatcgc
     2941 cggatggggc
//
LOCUS       DASYLX010000271         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_339854,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000271 DASYLX010000000
VERSION     DASYLX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339854"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="hpt"
                     /locus_tag="VGP27_06600"
     CDS             <1..366
                     /gene="hpt"
                     /locus_tag="VGP27_06600"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV7851020.1"
                     /translation="QLEFMAVSSYGSSTSSSGVVRILKDLDRDINDRDVLIVEDIVDS
                     GLTLSWLMRNLATRHPRSLRVCTLMRKPDAVRDDVAIAYVGFDIPNEFVVGYGLDYAE
                     RYRDLSYIGTLDPKVYEEF"
     gene            371..>2227
                     /locus_tag="VGP27_06605"
     CDS             371..>2227
                     /locus_tag="VGP27_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7851021.1"
                     /translation="MTRTASAHARSETALRICPLCEATCGLTLTIQDGRVTGARGDRE
                     DVFSAGFICPKGASLGELDNDPDRLTRPMVRRDGVLVDATWDEAFAVVAERLGDVIRE
                     HGGTSVGVYLGNPNAHTVAGSLYPPLIIRGLGTRQAFSASTLDQMPKHVALGHMFGSP
                     VAFTVPDLDRTDYLVIIGANPLVSNGSLATAADFPGKLRALRKRGGRLTVIDPARTRT
                     AELADRHIAPRPGTDAALLFAVVHVLFEEGLVATDLGGIADLVNGLDDVRRLAEQFSP
                     EDVDDYCGVPAEDIRALARELAAAPTAAVYGRIGTSTVEFGTLASWLVDVINVVMGNL
                     DRPGGVMFPLGATAPAPRPPKPGRGFKTGRWHSRVSGYPEALSEMPAAAMAEEIDTPG
                     DGQIKAMITIAGNPVLSAPDGDRLDRALGGGFMDFMISIDPYLNETTRHADVILPPPP
                     PSQSPHFDFALNNLAVRNNVRYSPPVLPLDGRPDEAEILSRIALTLYGIDVDGDPSLV
                     DDQVIATTLAKETADPDSPVADRPVDELTAMLATGPGYERRLDMMLRLGPYGDAFGAK
                     PDGLTLERLKAAPHGIDLGPLRPRLPEVLRTPSGRIELAPEPFTVDAARLREQ"
BASE COUNT          372 a          780 c          719 g          356 t
ORIGIN      
        1 cagctggaat tcatggcggt cagttcctac ggctcgtcga cgtcgtcgtc gggcgtggtg
       61 cgcatcctca aggatctcga ccgcgacatc aacgaccgcg acgtgctgat cgtcgaggac
      121 atcgtcgact ccgggctgac cctgtcctgg ctgatgcgca atctcgcgac ccgacacccg
      181 cggtcgctgc gggtgtgcac gctgatgcgc aagccggacg ccgtacgcga cgacgtcgcc
      241 atcgcctacg tcggcttcga catccccaac gaattcgtcg tcggctacgg cctggactac
      301 gccgagcgct accgcgacct gtcctatatc ggcacgctcg acccgaaggt gtacgaggag
      361 ttctaggagc atgacccgga ccgcctccgc acacgcgagg agcgaaaccg ctctccggat
      421 ctgcccgttg tgcgaggcca cctgcgggct gacgctgacg atccaggacg gccgggtgac
      481 cggggcgcga ggcgaccggg aggatgtctt cagcgccggg ttcatctgcc ccaagggtgc
      541 cagtctcggc gagctggaca acgaccccga tcggctgaca cggcccatgg tgcgtcgcga
      601 cggcgtgttg gtcgatgcga cgtgggacga ggcgttcgcc gtcgtcgccg agcggctggg
      661 tgacgtcatt cgcgagcacg gcggcacgtc cgtcggcgtg tacctgggca accccaacgc
      721 gcacaccgtg gcgggcagcc tgtacccgcc gctgatcatc cgcgggctgg gcacccgtca
      781 ggcgttctcc gccagtactc tcgatcagat gcccaaacac gttgcgcttg gccacatgtt
      841 cggcagcccg gtcgcgttca cggtgcccga tttggaccgt accgactact tggtgatcat
      901 cggagcgaat ccacttgtct ccaacggaag tctggccacc gctgccgact tccccggcaa
      961 gctgcgcgca ctgcgcaagc gcggcggccg gctcaccgtt atcgacccgg cacgcacccg
     1021 cacggcagaa ctggccgacc gccacatcgc ccctcggccg ggcaccgacg ctgcactgct
     1081 gttcgcagtt gtgcatgtgc tcttcgaaga gggccttgtg gcaacggatc tcggcggtat
     1141 cgccgacctc gtcaacggac tcgacgacgt tcggcgcctc gccgagcaat tctcgccgga
     1201 ggacgtcgac gactactgcg gtgtaccggc cgaggacatc cgggcgctcg cccgcgaact
     1261 ggccgcggcg ccgacggccg ccgtgtacgg ccgaatcggc acgtcgacag tcgaattcgg
     1321 cacgctcgcg agttggctgg tcgacgtcat caacgttgtg atgggcaatc tcgaccgacc
     1381 cggcggggtg atgttcccgc tcggggcgac ggcgccagcc ccgcggccgc cgaaaccggg
     1441 ccgggggttc aagaccggcc gctggcacag ccgggtgtcg ggctatcccg aggcattgtc
     1501 cgaaatgcca gccgccgcaa tggctgagga gatcgacact cccggggacg ggcagatcaa
     1561 ggcgatgatc acgatcgccg ggaacccggt tctgtcggcg cccgacgggg accgcctcga
     1621 ccgggcgctg gggggaggat tcatggactt catgatttcc atcgatccct acctcaacga
     1681 gaccacccgg catgccgacg tcatccttcc gccaccgccg ccgtcgcaga gtccccattt
     1741 cgacttcgca ctgaacaatc tcgcggtgcg aaacaacgtg cgctactcgc cgccggttct
     1801 gccgctcgac gggcgaccgg acgaagcgga gattctgtcg cgaatcgcgt tgactctcta
     1861 cggcatcgac gtcgacggcg atccctcgct tgtcgacgac caggtcatcg cgacgacgct
     1921 ggccaaggag accgccgacc ccgactcacc tgtggcagac aggccggtcg acgagttgac
     1981 cgcgatgctc gcgaccggcc ccggctacga acgacggctc gacatgatgc ttcggctcgg
     2041 accctacggc gatgcgttcg gcgccaagcc agacggcctc acccttgaac ggctcaaggc
     2101 cgcaccgcac ggcatcgacc tgggaccgct gcgaccccgg ttgcctgaag tgctccgaac
     2161 accaagcggg cgaatcgaac tcgcgccgga gccgttcacc gtggacgccg cgcggcttcg
     2221 cgagcag
//
LOCUS       DASYLX010000272         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3631255,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000272 DASYLX010000000
VERSION     DASYLX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3631255"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGP27_06610"
     CDS             <1..484
                     /locus_tag="VGP27_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7851022.1"
                     /translation="FSKELGGQQVTVLNDADAAGLAEEKFGAGRDNTGVIVLLTFGTG
                     IGSAVIHNGALLPNTEFGHIEVGGKEAEHRAASSVKESRGWSYERWAKEVTKVLVAIE
                     NAIWPDLFIAGGGISRKADKWIPLLTNRTPIVAATLLNTAGIVGAAMAADIDVTSTAK
                     "
     gene            679..>1746
                     /locus_tag="VGP27_06615"
     CDS             679..>1746
                     /locus_tag="VGP27_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7851023.1"
                     /translation="MAATKASPAATKPVKRTATKTPAKKATAAKTTNGSAPARPAKRA
                     TKAAAGTAKTDTPAAKSHAAAKPAKASARAKADGAAPRSRAKKATAAEPTTKVDVADD
                     ELATTDELDGEPEVLVDEDLELEEIDDDAGEDEATDAAEGEAGTKAVKGAAAATPAAA
                     DDDEIAEPSEKDKASGDFVWDEEESEALRQARKDAELTASADSVRAYLKQIGKVALLN
                     AEEEVELAKRIEAGLYATQLMAEIADKGEKLPAAQRRDMAWICRDGDRAKNHLLEANL
                     RLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRA
                     MADQARTIRIPVHMVEVINKLG"
BASE COUNT          386 a          581 c          575 g          204 t
ORIGIN      
        1 cttcagcaag gaactcggcg gccagcaggt gaccgtgctc aacgacgccg acgccgccgg
       61 gctcgccgag gagaagttcg gcgcgggccg cgacaacacc ggtgtgatcg tgctgctgac
      121 gttcggcacc ggcatcgggt cggctgtcat ccacaacggc gcactcctgc ccaataccga
      181 gttcggccac atcgaggtcg gcgggaaaga ggccgagcac cgggccgcgt cctcggtcaa
      241 ggagagcagg ggctggagct acgagcgctg ggccaaggag gtcaccaagg tgctggtggc
      301 cattgagaac gccatttggc cggatctgtt catcgcaggc ggcggcatca gccgcaaggc
      361 cgacaagtgg attccgctgc tgacgaaccg cacgccgatc gtggccgcga ccctgctgaa
      421 cacggccgga atcgtcggag cggcgatggc cgcggacatc gatgtcacct ctactgccaa
      481 gtagccacgc cgaagagcgc caaaattacc gctcggcgcg gcgatatcga acactgtcgt
      541 tacaatggtc gacggcggcc gcgaccagaa agcggcgagt atccccagca gatatcaacg
      601 ccgcattcga catcgcagac gctttccgat ggtgcatgcc cccagggcca tcggaatcct
      661 catgaccgaa agggtgacgt ggcagcgaca aaggcaagcc cggcagccac aaagccggtg
      721 aagcgcaccg ctaccaagac tcccgccaag aaggccacgg cagccaagac caccaacggg
      781 tccgcacctg ccagacccgc caagcgagcc accaaggcgg ccgccggcac ggcgaagacg
      841 gacacgccgg cagccaagag ccacgcggca gccaagccgg ccaaagcctc ggcgcgggcc
      901 aaggccgacg gcgcggcccc gcgcagccgc gccaagaagg cgaccgcggc ggagccgacc
      961 accaaagtcg acgtcgccga cgacgagctg gcgaccaccg acgaactcga cggtgagccc
     1021 gaagtactcg tcgacgagga tctcgaactc gaggaaatcg acgacgatgc cggtgaggac
     1081 gaggcgaccg acgcggccga aggtgaggca ggcacgaagg cggtgaaggg cgccgctgcg
     1141 gcaacacctg ctgccgccga cgacgacgag atcgccgaac ccagcgagaa agacaaggct
     1201 tcgggcgact tcgtctggga cgaagaggaa tccgaggcgc tacggcaggc ccgcaaggac
     1261 gccgagctca cggcgtcggc cgactcggtg cgcgcctatc tcaagcagat cggcaaagtc
     1321 gcactgctca acgccgaaga agaggtcgag ctggcgaagc gaatcgaggc cggtctctac
     1381 gccacccagc tgatggcgga aatcgccgac aagggcgaga agctgccggc cgcgcagcgg
     1441 cgcgacatgg cgtggatctg ccgcgacggc gatcgcgcga aaaaccatct gctggaagcg
     1501 aacctgcgac tcgtcgtatc gctggccaag cgctacaccg gccgcggcat ggcgtttctc
     1561 gacctcatcc aggagggcaa cctcggattg atccgtgcgg tcgagaagtt cgactacaca
     1621 aagggttaca agttctccac ctacgccacc tggtggatcc ggcaggccat cacccgcgcg
     1681 atggccgacc aggcccgcac catccgcatc ccggtgcaca tggtcgaggt gatcaacaag
     1741 ctcggc
//
LOCUS       DASYLX010000273         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_779505,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000273 DASYLX010000000
VERSION     DASYLX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779505"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGP27_06620"
     CDS             <1..902
                     /locus_tag="VGP27_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851024.1"
                     /translation="LAAAVVISFLRKERIGPLWLAAVGYAIACQIPIYLLRSSKFTAL
                     ELAQTLRYFPDLVVVLALLAAVGFCAPNRGRTRWLDASPTRTVVTVTAAVAFLASSLY
                     STKTFTTIWQDNPAQPYLQNAQAGLAKAHQTSSAPMLDQEVDPFVLQRVAAPENMASH
                     MFALLHDRPEFASTTTQLRMIDNRGHLVDAKVTWVRTIVRGPDPKCGYLVQPDFPVRM
                     PLDGPLLPADWTAEINYLANSEGSMTMSLSEGPDTRVPVHPGLNRVFVRLPGAGDAIN
                     VRANTAALSVCVASGPVGYLAPL"
     gene            918..1280
                     /locus_tag="VGP27_06625"
     CDS             918..1280
                     /locus_tag="VGP27_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7851025.1"
                     /translation="MTDVRETGMNVFRELLPDLQGEAGLPRGGFADELLEIGIDNVFG
                     RLWGRDGLSRRDRSLVTLGMLIALRATDELAIHFKIARTNGLTDDEIAEVIYHASGYA
                     GFPAAATANRIAGEALSD"
     gene            complement(1394..1466)
                     /locus_tag="VGP27_06630"
     tRNA            complement(1394..1466)
                     /locus_tag="VGP27_06630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Thr,seq:cgt)
     gene            complement(1524..>2298)
                     /locus_tag="VGP27_06635"
     CDS             complement(1524..>2298)
                     /locus_tag="VGP27_06635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV7851026.1"
                     /translation="EEPPPSTVFLLCAPSVDPEDISITLRSRCRHVALVTPRSEAIAQ
                     VLMDSDGLPAEDAKWAASVSGGHVGRARRLATDEQARRRRERALGLARDAATPSRAYG
                     AAEELVATAEAEALAQTADRNEAETEELRTALGAGGTGKGTAGTLRGATGAIKDLERR
                     QKSRQTRASRDALDRALIDLATYFRDALLVSAHAGEIQPNHPDMSDKVAAMAAAVPPD
                     RLLRCIEAVLDCREALAVNVKPKFAVDAMVATVGRALRN"
BASE COUNT          390 a          864 c          675 g          369 t
ORIGIN      
        1 tgcttgccgc cgcagtcgtc atcagcttcc tgcggaaaga gcgcatcggc ccgctgtggc
       61 tcgccgccgt cggctacgcg atcgcctgcc agatccctat ctacctcctg cggtcctcga
      121 agttcacggc gctcgaactc gcccaaaccc ttcgctactt ccccgatctc gtcgtcgtcc
      181 tcgccctcct cgccgccgtc ggattctgcg cacccaaccg cggacggacc cgatggctcg
      241 acgcctcccc cacccggaca gtcgtaactg tcaccgccgc agtcgctttc cttgctagca
      301 gcctctactc gaccaagaca ttcaccacga tctggcagga caacccggca cagccctact
      361 tgcagaacgc ccaagccggc ctcgcgaagg cgcaccaaac gtcgagcgct ccgatgctcg
      421 accaggaggt cgacccgttc gtccttcaac gcgttgccgc acccgaaaac atggccagcc
      481 acatgttcgc actactgcac gatcgacccg aattcgcaag caccacaact caactccgaa
      541 tgatcgacaa ccgcggtcac ctggtcgacg ccaaagtcac ctgggtgcgc actatcgtca
      601 ggggtccgga cccgaagtgc ggctacctcg tccagcccga cttccctgta cgcatgccgc
      661 tcgacggccc gctactcccg gccgactgga ccgccgagat caactacctc gccaacagcg
      721 agggttcgat gacgatgtcg ctcagcgaag ggcccgacac cagggtgccc gtgcatcctg
      781 ggctcaaccg cgtcttcgtc cgccttcccg gcgccggaga cgccatcaac gtgcgcgcca
      841 acaccgccgc tctgtccgtc tgcgtcgcat caggcccggt gggttacctg gcgcccctgt
      901 gatgccatcg tggacccatg actgatgtgc gtgaaacagg aatgaacgtc ttccgagaac
      961 tgctgccgga ccttcaaggc gaggcgggtc tgcctcgtgg cggattcgcc gacgagttgc
     1021 tcgagatcgg catcgacaac gtcttcggcc gcctctgggg acgcgacggc ctgagtcgcc
     1081 gcgaccgcag ccttgtgacg cttggcatgc tgatcgcgct gcgagccacc gatgagctcg
     1141 ccatccactt caagattgcc cgcaccaacg gcctgaccga cgacgagatc gccgaggtga
     1201 tctatcacgc cagcggctac gccgggttcc cggccgcggc gacggccaac aggatcgccg
     1261 gggaagctct gagcgactag tgcacgctca cggctaacga gagtgcggag tctgccggtg
     1321 aattgcggcg tgtcgggtgc agacacgcac gctcgcgggg caactgagcc acggggtaag
     1381 cgtggggcag gtgagccacc taggagaatc gaactcctga cctattcatt acgagtgaat
     1441 cgctctaccg actgagctaa ggtggcgcgc ccggcgctga ccgggcggca cgagtctacg
     1501 acaggcctcg gcgagcaccc aagtcagttc cgcagcgccc gacccaccgt ggcgaccatc
     1561 gcgtcgaccg cgaacttcgg cttcacgttg acggccagcg cctcacgaca gtccaagaca
     1621 gcctcgatac accgcaataa tcgatcgggc ggcaccgcgg ccgccatcgc cgccaccttg
     1681 tccgacatgt ccgggtgatt cggctggatc tcgcccgcgt gcgccgacac cagcagcgca
     1741 tcccggaaat aggtcgcgag gtcgataagc gcccggtcca gcgcatcgcg cgacgcccgt
     1801 gtctgacgcg acttctgtct gcgctcaagg tctttgatcg cgcccgtcgc accgcgcagc
     1861 gtgccagcgg tgcccttgcc ggtaccgcct gcacccagcg ccgtgcgcaa ctcctcggtc
     1921 tcggcctcgt tgcggtcggc cgtctgcgcc aacgcttcgg cctccgctgt ggccaccagc
     1981 tcctcagcgg caccgtaggc ccgcgacggc gtcgccgcat cgcgcgccaa ccccaacgcg
     2041 cgctcccgtc gccggcgcgc ctgctcgtcg gtggccagcc gccgcgcgcg ccccacatgc
     2101 ccgccgctga ccgacgccgc ccacttggcg tcttcggcgg gcagcccgtc cgagtccatc
     2161 agcacctgcg cgatcgcctc ggaacgcggc gtaaccaggg ccacgtgcct gcagcgcgac
     2221 cgcagggtga tcgagatgtc ctccggatcc accgacggcg cgcacagcag gaacactgtc
     2281 gacggtggcg gctcctct
//
LOCUS       DASYLX010000274         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_779514,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000274 DASYLX010000000
VERSION     DASYLX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779514"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGP27_06640"
     CDS             <1..214
                     /locus_tag="VGP27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851027.1"
                     /translation="ELNVEEGVVFNAEIAAAIQENLAESNYGEVLAKRAITTVSLNDS
                     GEIVEHHPDGSAVVLTTGRRAARRRK"
     gene            231..803
                     /locus_tag="VGP27_06645"
     CDS             231..803
                     /locus_tag="VGP27_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV7851028.1"
                     /translation="MNRLDLVVGPNGAGKSTFVELTLASLLPRSVFVNADEIARQRWP
                     DDPVGHAYDAARVAAETRAMLIELRRSFIAETVFSHPSKLELIDAAHAADYTVMLHVL
                     LIPQELAVQRVRHRVSAGGHDVPEDKIRGRYQRLWPLVAAAIVRSDSATVYDNSGLKG
                     PRIVAQFSGGFAVGLSAWPDWTPKELSTRW"
     gene            995..1402
                     /locus_tag="VGP27_06650"
     CDS             995..1402
                     /locus_tag="VGP27_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851029.1"
                     /translation="MIRLGIALSATLTLALAGIIGASAAQADPWPPSPCGFSISPPQV
                     VQVDGVSKVTATVTSTVCGIAASPYQSVACVEQSGRDAHSSCMQGTDTAQAYFEPYTP
                     GATYTSRGRGCGIIVPTLPADNCQILGPLTATL"
     gene            1423..2175
                     /locus_tag="VGP27_06655"
     CDS             1423..2175
                     /locus_tag="VGP27_06655"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7851030.1"
                     /translation="MSDRVLVQVENHVALITLNDPDRRNAVTAEISAALRAAVDAAEA
                     NPGVHAVIVTGAGKSFCAGADLTALGEATEDGLRRIYDGFLAVANCALPTIAAVNGAA
                     VGAGLNLALAADVRIAGPAALFDPRFQKLGIHPGGGATWMLQRAVGVQAARASLLFGM
                     RFDAEAAVRYGLALDVADDPVAAAKELAAGPAAAPRDVVLATKASMRATANPGSIDVE
                     QHAIAVTTELKPQADSILSPEFAERLAAAKRK"
     gene            complement(2200..2667)
                     /locus_tag="VGP27_06660"
     CDS             complement(2200..2667)
                     /locus_tag="VGP27_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851031.1"
                     /translation="MTTHDQHTEVDQFDEDRAMTTEARQTDIGSPAPSSVPNQSHTPA
                     SETPTVAMKPIGGTQDEAESSTEEPLFGGDELAALRAQWDSVQAEFVDNPKDCVGKAD
                     SLVSELIEKLTAGFADARSRLEQQWGQGETVSTEDLRVALRRYRSFFERLLSA"
     gene            complement(2664..3239)
                     /locus_tag="VGP27_06665"
     CDS             complement(2664..3239)
                     /locus_tag="VGP27_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851032.1"
                     /translation="MPTWEWILIAAGAVIVVAAVIVGAARSSSRSRTHRLKQHYGPEY
                     EHLVSEAGDRKVAEKELTAREHNRDKLDIVALTPSALTHFTNHWHQVQTAFVDDPASA
                     VGAADRLVTEVMREKGYPVDDFEQREADISVDHPQIVENYRIAHGIHLSQMNGGVDTE
                     KQREAFVHYRALFERLLDAKKDNDTSQEATA"
     gene            complement(3338..3493)
                     /locus_tag="VGP27_06670"
     CDS             complement(3338..3493)
                     /locus_tag="VGP27_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HEV7851033.1"
                     /translation="MIILGIILLIAGFLLKISILWTIGIILIVIGAILAIVGSTGRAI
                     GGRRHYF"
BASE COUNT          653 a         1289 c         1138 g          697 t
ORIGIN      
        1 ggagctcaac gtcgaagaag gcgtcgtctt caatgccgag atcgccgccg caatccagga
       61 gaacctggcg gagtccaact acggcgaagt actcgccaag cgcgccatca ccacggtgtc
      121 cctcaatgac tccggtgaga tcgtcgaaca ccaccccgac ggcagcgctg ttgtgctgac
      181 gaccggccgc cgggcggctc ggcgcaggaa gtgaacgacg cggcacgtcg atgaaccggc
      241 tcgatctcgt tgtcggaccc aatggtgcgg gtaagtcgac cttcgtcgag ctgacgctgg
      301 catcgctact gccgcgaagc gtcttcgtca acgccgacga gatcgccaga cagcgctggc
      361 ccgacgaccc ggtcgggcac gcgtacgacg ccgctcgggt ggcggcggaa accagggcca
      421 tgctcatcga acttcggcgg tcgttcatcg ccgagacggt gttttcccat ccgtccaagc
      481 tcgaattgat cgacgccgcc cacgcggcgg attacaccgt catgctgcac gttctgctca
      541 tccctcagga gctcgctgtt caacgagtca gacatcgagt cagcgcgggc ggtcacgatg
      601 ttcccgaaga caagatccgc gggcgctatc aacgactttg gccactcgtc gccgccgcca
      661 tcgtccgatc cgactccgcc accgtctatg acaacagcgg cctgaagggc ccgcgcattg
      721 tggcgcagtt cagcggcggc ttcgccgtcg gcctttccgc gtggcccgat tggacaccga
      781 aggagttgtc cactcgatgg tgagccatac cgacggcact acaccttcaa ggccgaggcg
      841 taccgggaca cctatttcga ttctcccgtc gaccctcatc gctggcgcac tgacgcgccg
      901 agttctgcag gaaggttgtt cgggaggact tgagttcaag gcccagacgc agaagtcgat
      961 gtatgagccg gtggccctga tctaccatcc agacatgatt cggcttggca tcgccctgtc
     1021 ggcgacgctc accctggcgc ttgcgggcat catcggcgcg agcgccgcac aggccgaccc
     1081 ctggcccccg tcaccgtgcg gcttctcgat ctctccaccg caggtggtgc aggtggacgg
     1141 ggtcagcaag gtcaccgcga cggtgacgtc cactgtctgt ggtatcgcgg ccagcccgta
     1201 ccagagcgtg gcctgcgtcg agcaatcggg acgtgacgca catagctcgt gcatgcaggg
     1261 caccgacacc gcgcaggcct atttcgagcc ctacacgccc ggcgcgacct acacatcacg
     1321 cggccgcggc tgcggcatca tcgtgcctac cctgcctgcc gacaactgcc agatcctcgg
     1381 gcctctcacc gccaccctgt aggcgctcac tagcctgaag cgatgtcaga tcgcgtcctt
     1441 gtccaggtcg aaaaccacgt cgcgctcatc accctcaacg atcccgaccg ccgcaacgcc
     1501 gtcaccgctg agatctccgc agctctgcgc gcggcggtcg acgcggccga ggcgaacccc
     1561 ggcgtgcacg ccgtcatcgt caccggcgcc ggcaagtcgt tctgcgcagg cgccgacctg
     1621 actgcgctcg gcgaggcgac ggaggacggg ctgcggagaa tctacgacgg cttcctcgcg
     1681 gtggccaact gcgcactgcc gacgatcgcg gccgtcaacg gtgccgccgt cggcgccggg
     1741 ctcaacctcg cactcgccgc ggatgtgcgc atcgcgggtc ccgccgcgct gttcgacccg
     1801 cgtttccaga agctcgggat ccatcccggc ggcggcgcga catggatgct gcagcgcgcc
     1861 gtcggcgtac aagccgcacg ggcctcgcta ctgttcggga tgcgattcga cgccgaggcc
     1921 gccgtgcgct acggattggc tctcgacgtc gccgacgacc cggtcgccgc cgccaaggag
     1981 ctcgccgcgg ggcctgcggc cgcaccccgc gacgtcgttc tcgccaccaa agcatccatg
     2041 cgcgccaccg ccaaccccgg ctcgatcgac gtagagcaac acgcgatcgc agtcacgact
     2101 gagctgaaac ctcaggcgga ttcgatactt tcacccgaat tcgccgaacg tctggccgcc
     2161 gccaagcgca agtagcgtat ctggcgaacg gctgcgctgt cacgccgaga gcagccgctc
     2221 gaagaatgat cgatagcgtc tcagagccac ccgcagatcc tctgtcgaca ccgtctcgcc
     2281 ctgtccccac tgctgctcga gccgtgaacg cgcatcggcg aagccggccg tgagcttctc
     2341 gatcaactcc gacaccaagc tgtcggcttt tccgacgcag tccttcggat tgtcgacgaa
     2401 ttccgcttgc acagagtccc actgcgcgcg cagggccgcc agctcgtcgc cgccgaatag
     2461 cggttcctca gtggatgatt cggcctcgtc ttgtgtccca ccaatcggct tcatcgccac
     2521 tgtcggcgtc tcgctcgctg gagtatgcga ttggttggga acgctggatg gcgccggcga
     2581 accgatgtcg gtctgtcggg cttccgtggt catcgcccgg tcttcgtcga actggtcgac
     2641 ctcggtgtgt tggtcgtggg tcgtcatgct gttgcctcct gtgaggtgtc gttgtccttc
     2701 ttcgcgtcaa gcaacctttc gaagagcgcc cggtaatgca cgaacgcctc gcgttgcttc
     2761 tcggtgtcca cgccgccgtt catctgtgac aagtggatgc catgggcgat gcggtagttc
     2821 tccacgatct gcgggtggtc caccgatatg tcggcttccc gctgctcgaa atcatcgaca
     2881 gggtagccct tctcgcgcat cacctcggtg accagccgat cggcagcgcc gaccgcactc
     2941 gccgggtcgt cgacgaatgc cgtctgcacc tggtgccagt gattcgtgaa atgtgtcaac
     3001 gccgatggcg tcaaggcgac gatgtcgagc ttgtcacggt tgtgttcacg agcggtgagc
     3061 tccttctcgg ccaccttgcg atcgccggct tcggaaacca ggtgctcgta ctcgggacca
     3121 taatgctgct tcagccggtg tgtcctgctg cggctactcg accttgcggc cccaacgatc
     3181 accgcggcga ccacgatgac tgcgccggct gcgatcagaa tccattccca tgtgggcatt
     3241 gcctgctcct ctcgtttctg agccaaaaag cctgagcgag gcgcgaatat ctactcgctc
     3301 ggcaaaagtt cctgacgtgt tgctgacttg aagccgatca gaagtagtgc cgccggcccc
     3361 cgatggcgcg gcccgtggag ccgacaatcg ccaggatcgc cccgatgacg atcaggatga
     3421 ttccgatggt ccagagaatg gagatcttga gcaggaatcc cgcgatcagc aggatgattc
     3481 cgaggatgat catgactggc ccttccgttg cgttgtgact gtcggacggt cgggaccatg
     3541 ccccgacact ggattcagtc gatcacccct aaggggggtg gtaatacacc cctaaggagg
     3601 taatctgcgc gatatgtcat cctcagcttc acgctcatcc gccccgatca cggtggcgct
     3661 cgtcgatgac tacgacatcg tcgtcaaggg cgtggccaac atgctcgacc cgtaccgcga
     3721 ccgcgtcgtc atcgccgaac tcgactccac catgcccgtc gacgacgcgg tggacat
//
LOCUS       DASYLX010000275         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3850603,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000275 DASYLX010000000
VERSION     DASYLX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850603"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VGP27_06675"
     CDS             complement(<1..155)
                     /locus_tag="VGP27_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEV7851034.1"
                     /translation="MSRAPTSAGREQRRAQLVLDDGRIFTGTSFGALGETLGEAVFST
                     GMSGYQET"
     gene            complement(152..655)
                     /locus_tag="VGP27_06680"
     CDS             complement(152..655)
                     /locus_tag="VGP27_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV7851035.1"
                     /translation="MSTGTLVASLIVAAVLAVAIGFAVQAMMRGWRHRAERQVELIGA
                     LPAMPDTVGPAIVPATKGLYVGSTLAPSWQDRIAVGDLGYRTKAVLTRYPEGIMLQRS
                     GGRPIWIPDESITAIRTERGIAGKALTHHGILAIRWRLPSGTEIDTGFRGNDRTQYAQ
                     WLGEEAA"
     gene            complement(652..1944)
                     /locus_tag="VGP27_06685"
     CDS             complement(652..1944)
                     /locus_tag="VGP27_06685"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV7851036.1"
                     /translation="MSVLIRGVQLYGEGDRADVLVDDGQIVGIGADLSIPDDADVVDA
                     TGQILLPGLVDLHTHLREPGREYAEDIETGSAAAALGGYTAVFAMANTNPVADSPVVT
                     DHVWYRGQQVGLVDVHPVGAVTMGLAGAQLTEMGLMAAGAGQVRMFSDDGICVHDPLV
                     MRRALEYATGLGVLIAQHAEEPRLTVGAVAHEGPNAARLGLAGWPRAAEESIVARDAL
                     LARDAGARVHICHASTAGTVEIIRWAKEQGISITAEVTPHHLLLDDNRLATYDGRNRV
                     NPPLRETSDAEALRQALVDGVIDCVATDHAPHAEHEKMCEFANARPGMLGLQTALSVV
                     VETMVQPGLFTWRDVARVMSENPARIVGLPDHGRPLEVGEPANLTVVDPDATWTVAGK
                     ELASRSDNTPYETMTLPGSVTLTLLRGRVTARDGKSPA"
     gene            complement(1941..2894)
                     /locus_tag="VGP27_06690"
     CDS             complement(1941..2894)
                     /locus_tag="VGP27_06690"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV7851037.1"
                     /translation="MSPRHLLSAADLTRDEATAILDDADRFRDSLLGREVKKLPTLRG
                     RTVITMFYENSTRTRVSFEVAGKWMSADVINVSSSGSSVAKGESLRDTALTLRAAGAD
                     ALIIRHPASGAAQQLAEWTATEDGGPSVINAGDGTHEHPTQALLDALTVRQRLGGIEG
                     KRVVIVGDVLHSRVARSNVLLLHTLGAEVVLVAPPTLLPVGVSDWPVTVSHDLDAELP
                     LADAVLMLRVQAERMTGGFFPSAREYSTLYGLSEKRQAMLPGNAVVLHPGPMLRGMEI
                     SSSVADSTQSAVLQQVSNGVHIRMAVLFHLMVGADVEAISA"
     gene            complement(2947..3528)
                     /gene="pyrR"
                     /locus_tag="VGP27_06695"
     CDS             complement(2947..3528)
                     /gene="pyrR"
                     /locus_tag="VGP27_06695"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV7851038.1"
                     /translation="MGATDSADTDRELMTAADVSRTISRIAHEIIEKTALDGPDAPRV
                     VLLGIPTRGVALAARLADKIREFAGVDVPHGGLDITLYRDDLNIKPPRPLEDTSIPDE
                     GIDGTVVVLVDDVLYSGRTVRAALDALRDVGRPRAVQLAVFVDRGHRELPLRADYVGK
                     NVPTSRSDNIKVRLTEHDGHDGVRIASHGGPVR"
     gene            3656..>4605
                     /locus_tag="VGP27_06700"
     CDS             3656..>4605
                     /locus_tag="VGP27_06700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7851039.1"
                     /translation="MNLDSNRASINEAIDAGLLAGAVTLIWQAGEVLQVGELGSRDVE
                     ARLPMQRDTLFRIASMTKPVTVAAAMTLVDEGRLALTDPVTKWLPELSELRVMTDVHG
                     SLDDTVPAERPITIDDLMTHRSGFAYAFSVPGALARAYGKLSHRQDQHGWLADLARLP
                     LAHQPGARLTYSVSTDVLGVALSRIEGKPLADVLSERILGPVGMPDTGFSVGPKGRRR
                     AATMYQLGADATLHHDSMGPAPITDPPLCSGGAGLWSTADDYLRFVRMLLGFGTLDGT
                     RVLSEESVRLMRTDRLTEEQRRFPFLGMPYWIGRGFGLNLS"
BASE COUNT          690 a         1566 c         1582 g          767 t
ORIGIN      
        1 gtctcctggt agcccgacat gccggtggag aacaccgcct cgccgagcgt ctcgcccagc
       61 gccccaaacg atgtgccggt gaagatcctg ccgtcgtcga ggaccaactg tgccctgcgc
      121 tgctcgcgac cagcgctcgt cggcgctcga ctcatgccgc ctcctccccc agccattggg
      181 cgtactgcgt gcggtcgttg ccgcggaacc cggtgtcgat ctccgtgccc gacggtagcc
      241 gccacctgat ggccaggatg ccgtggtggg tgagggcctt ccctgcgatg ccgcgctcgg
      301 tgcggatggc ggtgatcgac tcgtcgggta tccagatcgg acgcccgccg ctgcgctgca
      361 gcatgatgcc ctcgggatag cgggtcagca cggctttcgt gcggtaaccg aggtcgccga
      421 ccgcgatgcg atcctgccag ctcggcgcga gcgtgctgcc gacatagagg cccttggtcg
      481 ccgggacgat cgccggtccg accgtgtccg gcatcgcagg cagcgccccg atcagctcca
      541 cctggcgttc ggctcggtgc cgccacccgc gcatcatggc ctggacggcg aacccgatcg
      601 ccaccgcgag cacggcggcg acgatcagcg aagcaacgag cgtgccggta ctcatgctgg
      661 cgattttcca tctcgcgcgg tgaccctgcc acgcaggagc gtcaacgtca cagagcccgg
      721 cagcgtcatc gtttcgtacg gggtgttgtc cgatcggctg gccaactcct tgccggcgac
      781 ggtccacgtc gcatcggggt cgacgacggt gaggttggcg ggctcgccga cctccagcgg
      841 cctgccgtgg tcgggcaacc cgacgatgcg ggccgggttc tcgctcatca cccgggcgac
      901 gtcgcgccag gtgaaaagcc cgggttgcac catcgtctcg acgaccaccg acagcgccgt
      961 ctgcaggccc agcatgccgg gccgggcatt ggcgaactcg cacatcttct cgtgctcggc
     1021 gtgcggtgcg tggtcggtgg ccacgcagtc gatgacgccg tcgacgagcg cctgccgcag
     1081 agcctcggcg tcgctggtct cgcgcagcgg cgggttgacg cggttgcgcc cgtcgtaggt
     1141 ggccagccgg ttgtcgtcga gcaacagatg gtggggcgtc acctcggcgg tgatcgagat
     1201 gccttgttcc ttggcccatc tgatgatctc gacggtgcct gcagtcgaag cgtggcagat
     1261 gtgcacccgg gccccggcat cgcgcgcgag cagcgcgtcg cgtgccacga tcgactcctc
     1321 ggcggcgcgc ggccagccgg ccaggccgag acgggcggcg ttcgggcctt cgtgggctac
     1381 ggcgccgacg gtgagccgcg gctcctcggc gtgctgggcg atcagcacac ccagaccggt
     1441 ggcgtactcg agggcacgtc gcatcaccag cgggtcgtgg acgcagatgc cgtcgtcgga
     1501 gaacatccgg acctgccccg caccggcggc catcaatccc atttcggtca actgcgctcc
     1561 ggccaggccc atggtgaccg caccgaccgg atgcacgtcg accagcccga cctgctggcc
     1621 gcgataccag acgtggtcgg tgaccacggg gctgtcggcc accgggttgg tgttcgccat
     1681 tgcgaagacc gcggtgtaac cacccagagc ggctgccgcc gagccggtct cgatgtcttc
     1741 ggcgtattca cgtcccggct cgcgcaggtg cgtgtgcaga tcgaccaagc cgggcagcag
     1801 gatctggccg gtggcgtcga cgacgtcggc atcgtcgggg atcgacaggt ccgcgccgat
     1861 gccgacgatc tggccgtcgt cgaccaggac gtcggcccgg tcgccctcgc cgtagagctg
     1921 cacaccgcga atgagaacgc tcatgcgctg atcgcctcca catctgcgcc gaccatcagg
     1981 tggaacagca ccgccatcct gatatgcacg ccgttggaaa cctgttgcag cacagccgat
     2041 tgcgtcgaat ccgccaccga cgacgagatt tccatgcccc gcagcatggg tccgggatgc
     2101 agcaccaccg cgttgcccgg cagcatcgcc tgccgtttct ccgacagccc gtagagcgtc
     2161 gagtactccc gcgcggacgg gaagaacccg cccgtcatcc gttcggcctg cacccgcagc
     2221 atcagtaccg cgtcggcgag cggcaactcg gcgtcgaggt cgtgcgacac ggtcacgggc
     2281 cagtcggaca ccccgacggg caggagggtc ggcggcgcca ccaggaccac ctcggcgccg
     2341 agggtgtgca gcagtaggac gttggaccgg gccactcggc tgtgcaggac gtcgccgacg
     2401 atgacgaccc gcttgccctc gatgccgccg agccgttggc ggacggtgag cgcgtcgagc
     2461 agcgcctgcg tcggatgctc gtgggtgccg tcgcccgcgt tgatcaccga cgggccaccg
     2521 tcttcggtgg ccgtccactc ggccagttgc tgcgcggccc ccgatgcggg gtggcggatg
     2581 atcagggcgt cggccccggc ggcgcgcaac gtcagcgccg tgtcgcgaag cgactccccc
     2641 ttggccaccg acgagccgga cgagctgacg ttgatgacat cggcgctcat ccacttgccc
     2701 gccacctcga acgacacccg ggtgcgggtc gagttctcgt agaacatcgt gatcacggtg
     2761 cgtccgcgca gggtcggcag cttcttgact tcgcggccga gcagcgaatc gcggaaccgg
     2821 tcggcgtcgt cgaggatcgc ggtggcctcg tcgcgcgtca ggtcggctgc agacaacagg
     2881 tgccgtgggc tcatcggtac cgtctctccc ctgctcttcg agcgtggccc cacttcgcgc
     2941 gagcgctcat cggacgggac ctccgtgaga tgcgatccgc acgccgtcat ggccgtcgtg
     3001 ctcggtcagc cgcactttga tgttgtcgct gcgcgaggtc ggcacgttct tgccgacgta
     3061 atcggcacgc agcggcaact cgcggtggcc acgatcgacg aagacggcga gctgcaccgc
     3121 gcgcggacgt ccgacatcgc ggagcgcatc gagcgccgcg cgcactgtgc ggccggaata
     3181 caaaacgtcg tcgaccagga ccaccaccgt gccgtcgatg ccctcgtcgg gtatggacgt
     3241 gtcttccagt ggccgcggcg gtttgatgtt gaggtcgtcg cggtagagcg tgatgtcgag
     3301 tccgccgtgc gggacgtcga cgccggcgaa ctcgcggatc ttgtcggcca gcctggccgc
     3361 cagcgcgacg ccgcgagtcg gtatgccgag gaggacgacg cgcggagcat ccggaccgtc
     3421 aagcgcggtc ttctcgatga tctcgtgggc gatgcgggaa atggtccggc tgacatccgc
     3481 cgcggtcatc agttcccggt cggtgtctgc agagtcggta gcgcccaagt gcccctgacc
     3541 tccttctccg cctctctgga cggatcgtta aaggacgtcg aactgccggg cagcttagca
     3601 cctgtgaacg gagcggccgt cagcgacacc gacggcccgc agtaacctgg cgccggtgaa
     3661 tctcgacagc aaccgtgcgt ccataaacga ggcgatcgat gcgggcctgc tcgccggggc
     3721 ggtcacgctg atatggcagg ccggcgaggt actgcaggta ggcgagctcg gctcccgcga
     3781 cgtcgaggct cgcctgccga tgcagcgcga caccctgttc cgtatcgcgt cgatgacgaa
     3841 gccggtcacc gtggccgcgg cgatgacgct tgtcgacgaa ggcaggctgg ccctgaccga
     3901 tccggtcacg aagtggctgc cggaattgtc ggagctacgc gtgatgacgg atgtccacgg
     3961 ctctctggac gacaccgttc ccgccgagcg gccgatcacg atcgacgatc tgatgaccca
     4021 tcggagcggt ttcgcgtatg cgttctcggt acccggtgcg ctcgcacgcg cgtacggaaa
     4081 gctgtcgcat cgccaggacc agcacggttg gctggcggat ctggccagac ttccactggc
     4141 ccatcagccg ggcgcgcggt tgacctacag cgtctcgacc gatgtgctcg gcgtcgcgtt
     4201 gtctcggatc gaaggcaaac cgctggccga cgtgttgtcc gagcgcatcc tgggtccggt
     4261 cggcatgccc gacaccggat tcagcgtcgg cccgaagggt cgccggcgcg cggccacgat
     4321 gtatcagctc ggtgcagacg ccacactgca ccatgactcg atgggtcccg caccgatcac
     4381 ggatccgccg ttgtgcagcg gcggggccgg cctgtggtcg acggccgacg actacctgcg
     4441 gttcgtgcga atgcttctcg gcttcgggac gctcgacggg actcgggtgt tgtccgaaga
     4501 gtccgtgcga ttgatgcgca cggaccggct gaccgaggag cagaggcggt ttccctttct
     4561 ggggatgccc tattggatcg gtcgcgggtt cggcttgaac ttgtc
//
LOCUS       DASYLX010000276         5964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1657337,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000276 DASYLX010000000
VERSION     DASYLX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657337"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGP27_06705"
     CDS             complement(<1..666)
                     /locus_tag="VGP27_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851040.1"
                     /translation="MDFFGLDDDERVIAETAAAFAEKRLAPNALQWDKSHHFPVDELR
                     EAAELGMAAIYCGEDAGGSGLRRIDAVRIFEQLAAADPTVAAFLSIHNMCAWMVDTYG
                     THEQRKSWVPKLASMEAIASYCLTEPGAGSDAGALRTKAVRDGDHYVLDGVKQFISGA
                     GASDVYIVMARTSDPETKPGPRGISAFIVEKGTPGLSFGADEEKMGWHAQPTAQVIFE
                     GARI"
     gene            complement(683..2203)
                     /locus_tag="VGP27_06710"
     CDS             complement(683..2203)
                     /locus_tag="VGP27_06710"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7851041.1"
                     /translation="MTTQIPHFIEGRRSNLASTRTADVMNPSIGEVQAQVLLASAADV
                     DTAVAAAVEAQKEWGAWNPQRRARVMMKFVELVNTNVDELAELLSLEHGKTVPDSKGD
                     IQRGIEVIEFAIGIPHLLKGEFTEGAGTGIDVYSLRQPLGVVAGITPFNFPAMIPLWK
                     AGPALACGNAFILKPSERDPSVPLRLAELFVEAGLPPGVFQVVQGDKEAVDAILEHPD
                     IKAVGFVGSSDIAQYIYSNAAANGKRAQCFGGAKNHMIVMPDADLDQAVDALIGAGYG
                     SAGERCMAISVAVPVGEETANRLRARLVERINALRVGHSLDPKADYGPLVTEAALKRV
                     RDYIAQGVEAGAEAVVDGRERATDELTFDEADLSKGYFIGPTLFDHVTTDMSIYTDEI
                     FGPVLCMVRAKDYEEALRLPSEHEYGNGVAVFTRDGDTARDFVSRVQVGMVGVNVPIP
                     VPVAYHTFGGWKRSGFGDLNQHGPASIIFYTKVKTVTERWPSGIKDGAEFVIPTMK"
     gene            complement(2290..3165)
                     /locus_tag="VGP27_06715"
     CDS             complement(2290..3165)
                     /locus_tag="VGP27_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851042.1"
                     /translation="MDVGIHVPIFDIDGGTTAIAGELARVGAAVEASGANWLSFMDHF
                     FQIEPTGLPAESNMLEGYTTLGFLAAHTSTVSLGLLVTGVTYRHPGILAKTVTTLDVL
                     SGGRAVLGLGAAWFEREHRGLGVPYPPLAERFERLEEALQICDQMWDPDNNGPYTGRH
                     YQLAETLCSPQPINRPKVMIGGSGERKTLRLVAQYGDACNLFASSPEEVEHKLNVLRR
                     HCDDVGRDYDEIRKTVTANNPRHTPETRDAFVAQMAEYAKLGVQTAITTPTSGSPAAW
                     VDGMAPAFQQLAGLG"
     gene            complement(3327..3932)
                     /gene="rfbC"
                     /locus_tag="VGP27_06720"
     CDS             complement(3327..3932)
                     /gene="rfbC"
                     /locus_tag="VGP27_06720"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEV7851043.1"
                     /translation="MEARELAVPGAWEITPKQHTDDRGVFFEWFTDAGFRAFARHRLE
                     LRQANCSVSAAGVLRGLHFAQLPPSQAKYVTCVRGAVFDVAVDIRIGSPTFGQWDSVL
                     LDDRDRRSIYLSEGLAHGFLALQDDSTVMYMCSAGYDPDREHTIRATDPALGIDWPGT
                     GNVLSDRDAEAPTLDEVKAAGLLPTWDDTRAFVEELRRRDS"
     gene            complement(3934..4920)
                     /gene="rfbB"
                     /locus_tag="VGP27_06725"
     CDS             complement(3934..4920)
                     /gene="rfbB"
                     /locus_tag="VGP27_06725"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV7851044.1"
                     /translation="MRLLVTGGAGFIGANFVHSVAGEHAVTVLDALTYSGSRESLVPV
                     EDDIELIQGDIADAELMTKLVAHADAVVHFAAETHVDNSLADPAPFLHSNVIGTYTVL
                     EAVRRHGVRLHHISTDEVYGDLELDEPRRFTEGTPYNPSSPYSSTKAAADMLVRAWVR
                     SYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGSGHNVRDWIHVDDHNS
                     AVWRILQDGQIGRTYLIGSEGERDNLSVMRTILALMDRDPDDFDHVVDRVGHDLRYAI
                     DPSALRDELGWEPKHTEFEDGLRATIEWYRDNESWWRPIKDAVEDTYQNRGQ"
     gene            complement(4948..5799)
                     /locus_tag="VGP27_06730"
     CDS             complement(4948..5799)
                     /locus_tag="VGP27_06730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851045.1"
                     /translation="MTDAVSLKPDLGRYGVWTFGTPKPEQAVEIEKLGYGALWIGGSP
                     AGNLEYVEPILEQTETLQVATGIVNVWTAPAGEVAEAYHRIENAYPGRFLLGVGIGHP
                     EHTDEYRKPYDVLVEYLDALDAAKVPTSRRVVAALGPKVLKLAAQRSAGAHPYLTTPE
                     HTGSARELVGPTVFLAPEHKVVLTTDADEGREVGRQAVDFYLNLSNYLNNWKRLGFTD
                     KDVAKPGSDRLIDAVVAYGSADDIAKRLNEHLEAGADHVTIQVLGGWDKLLPTLKELA
                     GPLGLKG"
     gene            complement(5822..>5964)
                     /locus_tag="VGP27_06735"
     CDS             complement(5822..>5964)
                     /locus_tag="VGP27_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851046.1"
                     /translation="GTPDEIAARLKEHITAGADHVAVQVLTGPDKLVPALAELAGPLG
                     LS"
BASE COUNT          959 a         1924 c         1982 g         1099 t
ORIGIN      
        1 gatccgggcg ccctcgaaga tgacctgcgc ggtcggctgg gcgtgccagc ccatcttctc
       61 ctcgtcggcg ccgaaactga gcccgggagt gcccttttcg acgatgaacg ccgagatgcc
      121 gcgtgggccc ggcttggtct cggggtcgct ggttctggcc atcacgatgt acacgtcgga
      181 cgcgccggcc ccggagatga actgcttgac gccgtcgagc acgtaatggt cgccgtcacg
      241 tacggccttc gtgcgcagcg cgccggcgtc cgatccggcg ccgggttcgg tcaggcagta
      301 gctggcgatg gcttccatcg acgcgagctt cggcacccac gatttgcgtt gttcgtgtgt
      361 gccgtaggtg tccaccatcc acgcgcacat gttgtggatc gacaggaacg ccgcgacggt
      421 gggatcggcg gcggccagtt gctcgaagat gcggacggcg tcgatgcggc gcaggccgct
      481 gccgccggcg tcttcgccgc agtagatcgc cgccatgccc agctccgcag cctcacggag
      541 ctcgtcgacc gggaagtgat gcgacttgtc ccactgcaac gcatttgggg cgagacgctt
      601 ctcggcgaac gccgccgccg tctcggcgat gacgcgttcg tcgtcgtcca gaccgaagaa
      661 atccatgtgg actacttcag ctctacttca tggtcgggat gacgaactcg gcgccatcct
      721 tgatgccgga gggccaccgc tcggtcaccg tcttcacctt cgtgtagaag atgatcgatg
      781 cgggcccgtg ttggttcagg tctccgaagc cggaccgctt ccagccgccg aacgtgtggt
      841 aggccaccgg aacggggatc gggacgttga cgccgaccat gccgacctgc acccgcgaca
      901 cgaagtcacg cgcggtgtcg ccgtcgcggg tgaaaaccgc gacgccgttg ccgtactcgt
      961 gttcggaggg aaggcgcagg gcttcctcgt agtctttggc gcgcaccatg cacaacaccg
     1021 gcccgaagat ctcgtcggtg tagatcgaca tgtcggtggt gacgtgatcg aacagcgtgg
     1081 gcccgatgaa gtaacccttc gacaggtcgg cttcatcgaa cgtcagctcg tcagtggcgc
     1141 gttcgcgtcc gtcgaccacg gcctccgcgc cggcttccac gccctgggcg atgtagtcgc
     1201 gcacccgctt gagggccgct tcggtgacca ggggaccgta atcggccttg gggtcaaggc
     1261 tgtggccgac acgcagcgca ttgatgcgtt cgacgagcct ggcgcgcaac cggtttgccg
     1321 tctcttcgcc cacgggcacc gcgacgctga tggccatgca ccgctcgccg gcactgccgt
     1381 agccggcacc gatcagcgca tcgacggcct gatcgaggtc ggcgtcgggc atcacgatca
     1441 tgtgattctt cgcaccgccg aagcactgtg cgcgctttcc gttcgccgcg gcgttcgagt
     1501 agatgtactg cgcgatgtcg gagctgccga cgaagccgac ggccttgatg tcgggatgct
     1561 cgagtattgc gtcgacggct tccttgtcgc cctgcaccac ctggaacact cccggcggca
     1621 gaccggcctc gacgaagagt tccgccagcc gcagcgggac ggacgggtcg cgctcggacg
     1681 gcttgaggat gaacgcgttg ccgcacgcga gggcgggtcc ggccttccac agcggaatca
     1741 tcgcggggaa gttgaagggg gtgatgcctg cgaccacccc gagcggctgg cggagcgagt
     1801 acacgtcgat gccggtgccg gcgccctcgg tgaactcgcc cttcagcagg tgcgggatgc
     1861 cgatcgcgaa ttcgatgacc tcgataccgc gctggatgtc gcccttggag tcgggaaccg
     1921 ttttgccgtg ctccagtgag agcagttcgg ccagctcgtc gacattggtg ttgaccagct
     1981 cgacgaactt catcatcacg cgtgcccggc gctggggatt ccaggcgccc cattcctttt
     2041 gagcctcgac ggccgcggcc accgcggtgt cgacgtcggc ggccgacgcc aggagcactt
     2101 gagcctgtac ttcgccgatg cttgggttca tcacgtcggc ggttcgggtc gacgccaggt
     2161 tgctgcgtcg gccctcgatg aagtgtggaa tctgtgtggt catgaccatg ccctccggca
     2221 agaatttcga atactaggac atcctagtaa cgcgccggga cgccgcgcaa gggcctgccg
     2281 ataaagccgc tatccgagcc cggcgagctg ctggaaggcg ggcgccatgc cgtcgaccca
     2341 ggcagccggg gagccgctgg tcggcgtcgt gatcgcggtc tgcaccccca gtttcgcgta
     2401 ttcggccatc tgagcgacga atgcgtcgcg cgtctcggga gtgtgccgag gattgttggc
     2461 cgtcaccgtc ttccggattt cgtcgtagtc gcggccgacg tcgtcgcagt gccgccgcag
     2521 aacgttgagc ttgtgctcga cctcctcggg cgagctggcg aacagattgc acgcatcgcc
     2581 atactgggcc accagccgca gcgtcttgcg ctcaccgctg ccgccgatca tcaccttggg
     2641 tcgattgatc ggctgcggcg aacacagcgt ctccgccaat tgatagtgcc ggccggtgta
     2701 ggggccgttg ttgtccggat cccacatctg atcgcagatc tgcagcgcct cttcgagccg
     2761 ttcgaagcgt tcggccaacg gcgggtacgg cacgccgagc ccgcggtgct cgcgctcgaa
     2821 ccatgcggcg ccgagaccga ggaccgcgcg cccgcccgac agcacgtcca gggtggtgac
     2881 ggtcttggcg aggatgccgg gatggcggta ggtaacgccg gtgaccagca agccgaggct
     2941 gacggtcgag gtatgagcgg cgaggaatcc cagcgtcgtg tagccctcca gcatgttcga
     3001 ctctgcgggc agacccgtcg gctcgatttg aaagaagtgg tccatgaagg acagccagtt
     3061 ggcaccggag gcctcgacgg cggcgccgac cctggcaagt tcaccggcga tggcggtggt
     3121 gccgccgtcg atatcgaaga ttggcacgtg aatgccgacg tccataggag gctcctggtt
     3181 gagagggcac gacagcgggc ctgacggccc gcacactcct acaagttcaa tgtcgtcggt
     3241 tgaattccgg gtcgccgagt tttcctcgaa atcggcgcga tgaacccggc gaactgcgac
     3301 gggaccgatc tgtgcaggct cggcgctcag gagtcgcggc ggcggagctc ctcgacgaac
     3361 gcccgagtgt cgtcccacgt cgggaggagg cccgcggcct tgacctcgtc gagcgtcggc
     3421 gcctcggcgt ctcggtccga cagcacattg cccgtgccgg gccagtcaat gccaagggcg
     3481 gggtcggtag cccggatggt gtgctcgcgg tccgggtcgt atccggctga gcacatgtac
     3541 atcaccgtcg agtcgtcctg cagggcgagg aaaccgtgcg ccaggccctc gctgaggtaa
     3601 atcgagcggc ggtcgcggtc gtcgagcagc accgagtccc actgcccgaa agtgggtgag
     3661 ccgatacgaa tgtcgactgc gacgtcgaac accgctccac gtacgcaggt gacgtacttg
     3721 gcctggctcg ggggcaactg cgcgaagtgc agaccacgca gcacgccggc cgccgagacc
     3781 gagcaattgg cctggcgcag ctccaggcga tgtcgggcga atgcgcgaaa tccggcatcg
     3841 gtgaaccact cgaagaagac gccgcgatcg tcggtgtgct gtttcggcgt gatctcccat
     3901 gcgccgggaa cggcgagctc gcgtgcctcc acgttactga ccacggttct ggtatgtgtc
     3961 ttcgacggcg tcttttattg gccgccacca ggattcgttg tcccgatacc actcgatggt
     4021 ggcgcgcaag ccgtcttcga attcggtgtg cttgggctcc cacccgagtt cgtcgcgcag
     4081 cgccgacgga tcgatcgcgt accgcaagtc gtggccgacg cggtcgacga cgtggtcgaa
     4141 gtcgtcggga tcacggtcca tcaacgccag aatcgttcgc atcacggaca agttgtcacg
     4201 ttcgccctcg gatccgatca gatacgtgcg acctatctgg ccatcctgca ggatcctcca
     4261 caccgcgctg ttgtggtcgt cgacgtgaat ccagtcacgt acgttgtggc cgctgccgta
     4321 cagcttgggt cgcctgccgg tcagcacatt ggtgatctgc cgcggaatga acttctcgac
     4381 gtgctgatag gggccgtaat tgttggagca gttggagatc gtggcccgta caccatagga
     4441 gcgcacccat gctcgcacca gcatgtcggc ggctgccttg gtcgacgagt acgggctgga
     4501 cgggttgtac ggcgtgcctt cggtgaagcg gcgaggttcg tccagttcga gatccccgta
     4561 gacctcgtcg gtcgagatgt gatgcagccg caccccgtgc cggcgcaccg cctccaaaac
     4621 cgtgtaggtg ccgatcacat tggaatgcag aaacggcgca gggtcggcca gcgagttgtc
     4681 gacgtgggtc tcggctgcga agtgcacgac ggcatcggcg tgcgcaacga gcttcgtcat
     4741 caactcggcg tcggcgatat cgccctgaat cagttcgatg tcgtcctcga ctggcaccag
     4801 cgactcgcgg ctgccggaat acgtcagtgc gtcgagcacc gtcaccgcgt gctcacccgc
     4861 cacgctgtgc acgaaattgg cgccgatgaa ccccgcgcct ccggtgacca gcaaccgcat
     4921 ggaactaccg taacggtccg cgaagttcta gcccttcagc cccagcggcc cggccagctc
     4981 cttcagtgtc ggcagcagct tgtcccagcc gccgagcacc tggatcgtca cgtgatcggc
     5041 ccccgcctcc aagtgctcgt tgagacgctt ggcgatgtcg tcggcactgc cgtaggcgac
     5101 caccgcgtcg atcagccggt cgctgccggg cttcgccaca tccttgtcgg tgaagccgag
     5161 ccgcttccag ttgttgaggt agttgctcag gttcaggtaa aaatcgacgg cctgccggcc
     5221 gacctcgcgt ccctcgtccg catcggtggt cagcacgacc ttgtgctcgg gcgccaaaaa
     5281 cacggtgggg ccgaccaatt cgcgggccga accggtgtgc tccggtgtcg tcagatatgg
     5341 gtgggcacct gcgctgcgct gcgccgccag cttgagcacc ttcggcccca gggccgccac
     5401 tacgcgtctg ctggtcggca ccttggcggc gtccagcgcg tcaaggtatt ccaccagcac
     5461 gtcgtagggc ttgcggtact cgtcggtgtg ctcgggatgg ccgatgccga cacccagcaa
     5521 gaaccggccg gggtaggcgt tctcgatccg gtggtacgcc tctgcgactt cacccgcggg
     5581 agccgtccag acgttgacga tgccggtcgc cacctgcagc gtctccgtct gctcgagaat
     5641 cggctcgacg tactcgagat tgcccgcggg ggagccgccg atccacaggg cgccgtatcc
     5701 gagcttttcg atctcgacgg cctgttcggg tttaggggtc ccgaaggtcc acactccgta
     5761 tcggccgaga tctggcttga gtgaaacggc gtcggtcatt gcgggtcccc ctgtgtgtcg
     5821 actacgaaag gccgagcggc ccggcgagtt cggcgagcgc agggaccagc ttgtcaggtc
     5881 cggtcagcac ctgcaccgcc acatggtctg cgccggccgt gatgtgctcc ttcagccgcg
     5941 cggcgatctc gtcgggggtg ccgt
//
LOCUS       DASYLX010000277         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1876221,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000277 DASYLX010000000
VERSION     DASYLX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876221"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGP27_06740"
     CDS             <1..556
                     /locus_tag="VGP27_06740"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV7851047.1"
                     /translation="NGGLPDDADLLVTVRASTLRHHAGQAAFPGGAADPGDDGPIHTA
                     LREANEETGIDTARLQPLATMEKMFIPPSGFHVMPVLAYSPDPGPVAVVDPSETAIVA
                     RIPVRAFINPENRLMVYRKQNTRRLAGPAFLLNQMLVWGFTGQVISAMLDVAGWAQPW
                     NTEDVRELDQAMTLVGHDGTDGEA"
     gene            559..>1677
                     /gene="marP"
                     /locus_tag="VGP27_06745"
     CDS             559..>1677
                     /gene="marP"
                     /locus_tag="VGP27_06745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance serine protease MarP"
                     /protein_id="HEV7851048.1"
                     /translation="MTSSQWLDMAVLAVAFVAGISGWRSGALGSLLSFIGVVLGAVAG
                     VLLAPHIVTHFTGTRTQLFITLFLILGLVVIGEIAGVVLGRAVRGAIRSRTVRLFDSV
                     IGVGVMVLAVAVVAYLLATPLTSTNQPNLAAAVRGSKVLSQVDSVAPPWLKAVPNRLK
                     GLLDTSGLPDVLQPFGRTPIANVDAPDAALINDPVVAAVRPSVTKIRGVAPGCQKVLE
                     GTGFVVAPNRVMSNAHVVAGAETVTVEADGASYDATVVSYDPDADISILDVPGLPSAP
                     LPFAQEPAKSGTDALVLGYPGGGDFQATPARVRETIELKGPDIYKTKTVDREVYTIRG
                     TVRQGNSGGPMIDRQGRVLGVVFGAAVDDAETGFVLTAR"
BASE COUNT          255 a          552 c          583 g          287 t
ORIGIN      
        1 gaacggcgga ctgcccgacg atgccgacct gctggtgacc gtccgggctt cgacattgcg
       61 ccaccacgcc ggccaggccg cgtttcccgg cggagcggcc gatcccggcg acgacggtcc
      121 gatacacacc gcgctgcgtg aggccaacga ggaaaccggc atcgacaccg cccgcctgca
      181 gccgctcgcc acgatggaga agatgttcat cccgccgtcg ggattccacg taatgccggt
      241 cctcgcctac tcgccggacc cagggccggt cgccgtcgtc gacccatcgg agacggcgat
      301 cgtggcgcgg atacctgtgc gcgcattcat caaccccgag aaccggctga tggtgtaccg
      361 caagcagaac acgcggcgcc tcgccgggcc tgccttcttg ctgaatcaga tgctggtctg
      421 gggtttcacc gggcaggtga tctccgcgat gctcgacgtg gcggggtggg cgcagccctg
      481 gaacaccgaa gacgtgcggg agttggacca agcgatgacc ctcgtcggtc atgatggcac
      541 cgacggggag gcatgactat gacgtcgtcg cagtggctcg acatggctgt tctggcggtc
      601 gcattcgtcg ccgggatctc cggatggcgt tcgggggcgc tggggtcgct gctgtccttc
      661 atcggcgtgg tgctgggagc tgtggccggg gtgctgctcg cgccgcacat cgtcacccac
      721 ttcaccggca cacgtacgca gctgttcatc acgctgttct tgatccttgg actggtggtg
      781 atcggcgaga ttgcgggcgt tgtgctcggc cgggcggtgc gcggtgcgat ccgcagccgc
      841 acggtgcgcc tcttcgactc ggtgatcggt gtcggcgtga tggtgttggc cgtggcggtg
      901 gtggcctatc tgctggcgac cccgctcacg tcgaccaacc agccgaacct ggctgcggcg
      961 gtgcgcggat cgaaggtgct gagccaggtc gactcggtcg cgccgccctg gctgaaggcg
     1021 gtgcccaacc ggctcaaggg cctactggat acgtccggtc tgcccgatgt gctgcagccg
     1081 ttcggccgca cccccatcgc caacgtcgac gccccggatg cggcgctgat caacgacccg
     1141 gtcgtggccg ccgtgcggcc gagcgtcacc aagatccgtg gcgtcgcgcc cggctgccaa
     1201 aaggtcttgg agggaacggg tttcgtcgtc gcgccgaacc gggtgatgtc caacgcacac
     1261 gttgtcgctg gagccgagac ggtgacggtc gaggccgacg gcgcaagcta cgacgccacg
     1321 gtggtctcct atgatcccga cgccgatatc tcgatcctgg acgtgccggg tctgccgtcg
     1381 gcgccgctgc cgttcgccca ggagccggcc aagagcggaa cggacgcact ggtgctcggc
     1441 tatcccggcg gcggcgactt ccaggccacc cctgcgcggg tacgcgagac cattgagctc
     1501 aagggccccg acatctacaa gaccaagacc gtcgatcgcg aggtctacac aatcaggggc
     1561 actgtccggc aaggtaattc gggcggtccg atgatcgatc ggcaaggccg ggtcctcgga
     1621 gtggtcttcg gtgcggcggt cgacgatgcc gagaccggct tcgtgctgac cgccaga
//
LOCUS       DASYLX010000278         6847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_560212,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000278 DASYLX010000000
VERSION     DASYLX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560212"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1262
                     /locus_tag="VGP27_06750"
     CDS             243..1262
                     /locus_tag="VGP27_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEV7851049.1"
                     /translation="MTLASPKLEPALPLAKGPLSSTVLGLLACRPPQRSFTPVALPIG
                     DADPYGLDLHLALYVCYELHYRGFAGVDPRWEWNPVLLALRGQLEEVFLEAVRRDVGD
                     IADDDTAAAEMDALCVEPADGTGPSYYLRDEGTWEQMQEYFVHRSLYHLKEGDPHAWV
                     IPRLTGQAKASFVAIEFDEYGGGHGDRIHQQLFADLMEAAGLDSSYLGYFDAVPAESL
                     AAVNLMSMFGLHRELRGAAIGHFACTEITSPPGSRRLVDALQRLGAPAPCVRFYAEHV
                     EADAVHEQVVRVDVVGELVAAEPHLDRDVVFGIRAMDLVEGRLAAHMTDCWASGRSSL
                     RRPLG"
     gene            complement(1242..1460)
                     /locus_tag="VGP27_06755"
     CDS             complement(1242..1460)
                     /locus_tag="VGP27_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEV7851050.1"
                     /translation="MTDDARLVRVVPSGPVMVQGPVKIEMPDGSVVESDRFMVAICAC
                     RRSKNYPLCDTSHRKRQRGDPASTQRPA"
     gene            complement(1457..2149)
                     /locus_tag="VGP27_06760"
     CDS             complement(1457..2149)
                     /locus_tag="VGP27_06760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEV7851051.1"
                     /translation="MTTAYNDWDAPVVAEGVYTPQEDSQLLINVMEKTGLARGRRVAD
                     LCTGSGVVAIAAADQGASGVTAFDICPRAAHFARQQVLTAGVDVAVHLGSWARAVEFG
                     PYDLVVCNPPYIPHDPGVDSLELPSHIGPARAWDAGYDGRLVLDPLCASMRDLLAEGG
                     TMLLVQSEFAEPRKTLAALSSYGLDAEILAWEWIPFGPVLSARAKWLEDTGRLDPGRR
                     EEELVVIRADRA"
     gene            2264..2935
                     /locus_tag="VGP27_06765"
     CDS             2264..2935
                     /locus_tag="VGP27_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEV7851052.1"
                     /translation="MEISDITALPVRLGSAVRNRRLFHPIGVLAEGRLERLAPAGEGL
                     PLATGDVTGRVSKGAGLPGALPDIAGLAWRMPPQSPAATPWDVLLASSLGGGVGRVAL
                     SPVASWTGATFSSLMPFRFEGGVWWLRARLITPIDATGVALDTIRNRIRSTGLDFDID
                     QAAGTGGFLPLARLTLRNVMPDGNDVAFDPVLHTGPGVALAPGWLTGFRRAAYRRSRE
                     GRDAE"
     gene            complement(2939..3091)
                     /locus_tag="VGP27_06770"
     CDS             complement(2939..3091)
                     /locus_tag="VGP27_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851053.1"
                     /translation="MTVGKGIYDDETADEPKGGRPGPADEGRDGGMATRENAPDVAEP
                     DQEPTD"
     gene            3198..3782
                     /locus_tag="VGP27_06775"
     CDS             3198..3782
                     /locus_tag="VGP27_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV7851054.1"
                     /translation="MDALTFLREDHKSVLGMLEVLDGAPTGSGAHSSGLETMVTNLII
                     AESQHEAVEEQFFWPAVRDALDNGDELADEAIAQEQAGKKLLQRLEDGKPGDADYHDA
                     LQEFVKAGREHIAYEQDVVWPAFEATVSREEREKIGEKLETAKKAAPTRPHPDTPPNP
                     TVLKTMGMGAAIADHVRDAVSGRTEKNPPDPQQH"
     gene            complement(3839..4735)
                     /locus_tag="VGP27_06780"
     CDS             complement(3839..4735)
                     /locus_tag="VGP27_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEV7851055.1"
                     /translation="MFVHNKDLQFEVRVSRPDPRFASLLMEQFGGANGELTAALQYFI
                     QAFVLRQKNPKMYDLFMDIATEELSHLEMVGSTITMLLDGLNDDLKLANERCDWMPAV
                     ANADGREQAIHQVAVNPMFFVLSGGGPDAKDSAGNNWTGAFIDANGDPTVDLRNNLAA
                     ESRAKIVYEYLKQFTDDPGVQETLTFLMTREIAHYQQFTAALNELPVNFPPGTLSGDD
                     RFQNVAFNMSNGDDASSRGPWNEGQGPWPEGMKWKYVQKPEEEWLGTKLRENQGEKNN
                     PEGEPAVPSEKPFTHEQRVPTS"
     gene            complement(4823..5812)
                     /locus_tag="VGP27_06785"
     CDS             complement(4823..5812)
                     /locus_tag="VGP27_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851056.1"
                     /translation="MTRFGYTLMTEQSGPKELVRYAVSAERAGFDFEVSSDHFSPWLS
                     SQGHAPYAWSVLGAVAHATERVELFTYITCPTFRYHPAVVAQKAATLQILADGRFTLG
                     VGSGENLNEHVVGKRWPTVARRHEMLREAIQIIRELFGGELVDWKGEYFEVDSARIWD
                     VPETPVPIAAAVSGHRSVEMFAPLADHLIAVEPNKKLVDSWHEARRATGLPGNVRVIG
                     QIPVCWDPDRDAAIARAHDQFRWFAGGWSVNADLPTTAGFSAATQFVKPEDVAASIPC
                     GPDLDAIVDAVREYWEAGFTDIALVQVGDEGQEEFLEEAAGPLLEKLRSQAPR"
     gene            complement(5849..6313)
                     /locus_tag="VGP27_06790"
     CDS             complement(5849..6313)
                     /locus_tag="VGP27_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV7851057.1"
                     /translation="MAELKDQLRADLTAAMKSQDKLRTATLRMLLAAIQSEEVSGKES
                     RELSDADVLKVLARESKKRGESAEIYTQNGRGELAANEHAEARIIDEYLPTQLTEAEV
                     ADVVDTAIAQVSEANGEAPGMKQMGQVMKAATAIAAGKADGSRLSAAVKARL"
     gene            6419..>6847
                     /locus_tag="VGP27_06795"
     CDS             6419..>6847
                     /locus_tag="VGP27_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851058.1"
                     /translation="MVSMSSPGCPPPGYPPPGYPGYPPPGYQPYPPPGYQGYPPPGYP
                     GYPGYPPPGYRAPGYGGAPYAPPAIKPGVIPLRPLSLTDIFNGAFSYIRTNPKATLGL
                     TTIVVVIAQLIGLALQIGPLAAMGELAVLRGEEASRAAELG"
BASE COUNT         1122 a         2313 c         2168 g         1244 t
ORIGIN      
        1 ttggccttgc cgcctaaatc gtcgatcttg ttgctggctt tgtctccgac gcccatgaca
       61 cgttccttcg gttctcgcgc tcggacgttc cgagccacgc accttaccga gttccctcaa
      121 ggcggccctt cggaaacttc cggctgcctg agaggcgaaa tcgccctcca ttcagctgtc
      181 aatgaacaga cattccgcgc cgcctggttt ggctgcgcac agcgcaggga atcaattgct
      241 gcgtgacgtt ggcatcaccc aagctcgaac ccgctttgcc cttggcgaag gggcccctgt
      301 cgtcaaccgt tctcgggttg ctcgcctgcc gcccgccgca gcgctcgttc acccctgtcg
      361 ctctgccaat cggtgatgcc gacccgtacg ggctggatct gcacctggcc ctctacgtct
      421 gctacgagct gcattaccgc gggttcgccg gtgtcgatcc ccgctgggag tggaacccgg
      481 tgcttctggc gttgcgcggc caactcgagg aggtgttcct cgaagcggtc cgccgggacg
      541 tgggcgacat cgccgacgat gacaccgccg ccgcagagat ggacgcgttg tgcgtcgaac
      601 cggctgacgg cacgggcccc tcgtactatc tgcgcgatga gggcacctgg gagcagatgc
      661 aggagtactt cgtccatcgc tcgctgtacc acctgaagga gggcgatccg cacgcctggg
      721 tcatcccccg gctgacgggc caggccaagg cgtccttcgt cgccatcgaa ttcgacgagt
      781 acggcggcgg ccacggcgac cgaatccatc agcagctgtt cgccgatctg atggaggccg
      841 cagggctgga ctcgtcgtat ctcggctact tcgacgccgt gccggctgag tctctggcgg
      901 ccgtcaacct gatgtcgatg ttcgggctgc accgggagtt gcgcggcgcg gcaatagggc
      961 atttcgcgtg caccgagatc acctcgccgc cgggctcgcg gcggctggtc gatgcgctgc
     1021 agcggctcgg cgcaccggcg ccgtgtgtcc gcttctatgc cgagcacgtc gaagcagacg
     1081 ccgtccacga gcaggtggtt cgagtcgacg tggtaggcga gctcgtggcc gcggaaccgc
     1141 acctggatcg agacgtggta ttcggaatca gggcaatgga tttggtcgaa ggccgcctgg
     1201 ccgcgcacat gacggactgc tgggcgtccg gccggtcgtc gctacgcagg ccgctgggtt
     1261 gacgcggggt cgccccgttg acgttttcga tggctggtgt cgcacaacgg ataattcttc
     1321 gaacggcggc aggcgcagat cgccaccatg aagcgatcgg attcgacgac gctgccgtcg
     1381 ggcatctcga tcttcaccgg cccctgcacc atcacagggc cggacggcac cacccgcacc
     1441 aagcgggcgt cgtcggtcat gctctgtccg ccctgatcac gaccagctcc tcctccctgc
     1501 ggccggggtc gagccgtccg gtgtcctcga gccacttcgc ccgagccgac agcaccggtc
     1561 cgaacggaat ccattcccag gcaaggattt cggcgtcgag gccataactc gacagcgccg
     1621 ccagcgtctt gcgcggttcg gcgaactcgg attgcacgag cagcatcgtg ccgccctcgg
     1681 cgagcagatc ccgcatcgat gcgcacagcg gatcgagaac gagtcggccg tcgtagccgg
     1741 cgtcccacgc gcgagccggc ccgatgtgcg acggcaactc gagactatcg acgcccggat
     1801 cgtgaggaat atacggcgga ttgcagacca ccaggtcgta gggaccgaat tcgaccgcac
     1861 gtgcccacga gcccagatgc accgctacat cgacgccggc cgtcaaaacc tgttgccggg
     1921 cgaaatgtgc ggcgcgcggg cagatgtcga atgcggtgac cccggaggca ccctgatcgg
     1981 ccgccgcgat cgcgacgacc ccgctgcccg tgcacagatc agcgacccgg cgtccgcgcg
     2041 ccagccccgt cttctccatg acattgatga gcagttggga atcttcctgc ggcgtataga
     2101 cgccctcggc gacgaccggc gcatcccagt cgttgtaagc agtggtcaac gtcggccttt
     2161 caaacgtgat cgtgaaactt caagccctga attaatgccc gatatcggcg gtgttaaacc
     2221 agcgctatgg tttgctcggc gcggacgggg gcaaccagaa cgggtggaaa tctccgatat
     2281 caccgcactg ccggtgcgcc tgggctcggc tgtacgcaac cgtcggctgt tccatccgat
     2341 tggcgtgctc gccgagggca gactcgagcg gctcgcgccg gcgggcgaag gattgccctt
     2401 ggcgaccggc gacgtcaccg gccgcgtgtc gaagggcgca ggcctgccgg gcgcgcttcc
     2461 cgatatcgcc ggcctggcgt ggcgcatgcc gccgcagtcg cccgcggcga caccgtggga
     2521 cgttcttctc gcttcctccc tcggcggcgg cgtcggccgc gtcgcactct ctcccgtggc
     2581 gtcctggacg ggcgccacgt tctccagcct gatgccgttt cgcttcgagg gcggcgtctg
     2641 gtggttgcgg gcgaggctga taacacccat cgacgcgacg ggcgtggccc tcgacaccat
     2701 tcgcaacaga atccggtcca ccgggctgga tttcgacatc gatcaggcgg cgggtaccgg
     2761 tgggttcttg ccgttggcgc gcctgacgct gcgcaacgtc atgccggacg gcaacgacgt
     2821 tgcgttcgac cctgtactgc acaccggtcc cggcgtcgcg ctcgcacccg ggtggctgac
     2881 cggtttccgg cgtgccgcct accgacgaag ccgcgagggc cgcgacgccg agtagaggtc
     2941 agtccgtcgg ctcctgatcg ggctctgcga catcgggcgc gttctctcgc gtcgccatgc
     3001 cgccgtcccg accttcatcg gctggccctg gcctgccgcc cttgggctcg tcggccgttt
     3061 cgtcgtcgta gatcccttta ccgaccgtca tcacagcctc cttcgtcccg aaggagctac
     3121 ccctgtacaa cccgtttcaa tcgcccggcc gggggtatgt gcagcggacc cccgatcccg
     3181 gtgaaaggca acaacacatg gacgccttga cgttcctgcg agaagaccac aagagcgtgc
     3241 tgggcatgct ggaggtactc gatggtgcac ccacaggttc cggcgcccac agcagcgggc
     3301 tggagaccat ggtcaccaac ctgatcatcg ccgagtctca gcatgaggcc gtcgaggaac
     3361 agttcttctg gccggccgtg cgcgacgcac tcgacaacgg tgacgaactg gccgacgagg
     3421 cgatcgcgca agagcaggcc ggaaagaaac tgctgcagcg gctggaagac ggtaagcccg
     3481 gcgacgccga ctaccacgat gcgctacagg agttcgtcaa ggccggccgt gaacacatcg
     3541 cgtatgagca ggacgtggtg tggccggcct tcgaagcgac ggtcagccgc gaggaacgag
     3601 aaaagatcgg cgaaaagctg gagacggcta agaaagctgc gccgacgcgc ccacatcccg
     3661 acactccgcc gaacccgacc gtgctgaaga cgatgggcat gggtgcggca atcgcagatc
     3721 acgtacgcga cgcggtcagc ggacgcacgg aaaagaatcc gcccgatcca caacaacact
     3781 gactccgtac gtggggaccg gtgtcgccct acgacgacac cggacctcat cgcctgaatc
     3841 agcttgtcgg cacccgctgt tcgtgagtga acggcttctc actcggcacc gccggttcgc
     3901 cctcagggtt gttcttctcg ccctggttct cccgtagctt cgtgccaagc cactcttctt
     3961 ctggcttctg cacgtacttc cacttcatgc cctccggcca cggcccttgt ccctcattcc
     4021 atggcccgcg tgaggacgcg tcgtcgccgt tggacatgtt gaatgccacg ttctggaagc
     4081 gatcgtcgcc cgacagggtg ccgggcggga agttgaccgg gagttcgttg agtgcagcgg
     4141 tgaactgctg gtagtgggcg atctcccggg tcatcagaaa ggtcaacgtc tcctgcacgc
     4201 ccggatcgtc ggtgaactgc ttgaggtatt cgtagacgat cttcgcgcga gattcggcgg
     4261 caaggttgtt gcgcaggtct accgtcgggt ccccgttagc gtcgatgaac gcaccggtcc
     4321 agttgttgcc tgccgaatcc ttggcgtcag gaccgccgcc gctgaggacg aagaacatcg
     4381 ggttcaccgc aacctgatgg atcgcctgct cgcggccgtc agcgttggcc accgccggca
     4441 tccagtcaca ccgctcgttg gccagcttga gatcgtcgtt caggccgtca agcagcatcg
     4501 tgatcgtgga gccgaccatc tcgaggtggc tgagttcttc ggtcgcgatg tccatgaaca
     4561 agtcgtacat cttcggattc ttctgccgca gaacgaatgc ctggatgaag tactgcaacg
     4621 ccgcggtgag ttcgccgttg gcgccgccga actgctccat cagcaacgaa gcgaatctgg
     4681 gatcgggtcg gctcacccga acctcgaatt gaaggtcttt attgtggacg aacatgggcg
     4741 cctcccggct gttgacggac tgcagtcgca ctgtggtctg caggtcgggc cggtgtaccc
     4801 ggcaacgggc gcaccaaacg gactatctag gcgcttggct tcgcagcttt tccaacagcg
     4861 gtcccgccgc ttcttcgagg aactcttcct gcccctcgtc gccgacctgc accagcgcga
     4921 tgtcggtgaa cccagcctcc cagtactccc gcacagcgtc gacgatcgcg tcgagatccg
     4981 gaccgcacgg aatggatgcg gcgacatctt cgggcttgac gaattgcgtt gcggcactga
     5041 agccggccgt ggtgggcaga tcggcgttca ccgaccaacc gcctgcgaac cagcggaact
     5101 gatcgtgcgc ccgggcaatc gcggcgtccc ggtcggggtc ccagcagacc ggtatctggc
     5161 cgatcacccg tacgttaccg ggcagtcccg tcgcgcgccg tgcctcatgc caggagtcca
     5221 ccagtttctt gttgggttca acggcgatca gatggtcggc cagcggtgcg aacatctcga
     5281 ccgagcggtg ccccgatacc gccgccgcga tgggaacggg agtctccggg acgtcccaga
     5341 tccgggcgga atcgacctcg aagtactcac ctttccagtc gacgagctca ccgccgaaca
     5401 attcgcggat gatctgaatc gcttcgcgca gcatctcgtg acgccgcgcc accgtcggcc
     5461 accgtttgcc gacgacgtgc tcattgaggt tctcgccgct gccgacgccg agggtgaacc
     5521 ggccgtcggc gagaatctgc agcgtggccg ccttctgggc cacgacggcg gggtggtacc
     5581 gaaatgtcgg gcaggtgatg taggtgaata gctcgacccg ctcggtggcg tgagcgacgg
     5641 cgcccagcac cgaccacgcg tacggcgcgt ggccctgtga cgacagccac ggcgagaagt
     5701 ggtcactgga gacttcgaag tcgaaccctg cacgctccgc cgaaacggca taacggacaa
     5761 gctctttggg tccgctctgt tcggtcatca gggtgtagcc aaaacgcgtc atgacaccta
     5821 gttacccggg tgtcggacgc cgaaacggct acagcctggc cttgaccgcc gccgagagtc
     5881 gtgacccgtc ggccttaccc gcggcgatcg cggtcgcggc cttcatcacc tggcccatct
     5941 gtttcatgcc gggcgcctcc ccgttcgcct cgctgacctg tgcgatggcg gtgtcgacca
     6001 cgtcggccac ttccgcttcg gtcagctgcg taggcaggta ctcgtcgatg atccgggcct
     6061 cggcgtgttc gttggctgcc agttcgccgc ggccgttctg ggtgtagatc tcggccgatt
     6121 cgccgcgctt cttcgactcc cttgccaaca ccttgagcac gtcggcgtcg gatagttcgc
     6181 gcgactcctt accggagacc tcctcggatt ggatcgcggc cagcagcatc cgcagcgttg
     6241 ccgtgcgcaa cttgtcctgc gacttcatcg ccgcggtcag gtctgcccgc agctgatctt
     6301 tgagttccgc catgacgcag acgctaccgc caccagccga gtcggccggc gacaatctcg
     6361 cgccggtcgt cgccgacacc acctggtcgc gttgttaatt ttcgcggtga tcgacaggat
     6421 ggtttccatg agcagccctg ggtgcccacc gcccggctat ccgccgcccg gttaccccgg
     6481 ctacccgccg ccgggatatc agccctaccc tccacccggc taccagggct atccgccgcc
     6541 cggctatccc ggctatcccg gctacccgcc gccgggctat cgcgcgccgg gctacggcgg
     6601 ggcgccgtac gcgccacccg caatcaagcc gggcgtgatc ccactgcgcc cgctgagcct
     6661 gaccgacatc ttcaacggcg ccttctccta catccggacc aacccgaagg cgacgctcgg
     6721 gctgacgacg atcgtcgtgg tgatcgctca gctgatcggg ctggcactgc agatcgggcc
     6781 gctggccgcc atgggcgaac tcgccgtgct ccgcggtgag gaggcgtcga gggccgcgga
     6841 gctgggg
//
LOCUS       DASYLX010000279         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2096636,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000279 DASYLX010000000
VERSION     DASYLX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096636"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGP27_06800"
     CDS             complement(<1..330)
                     /locus_tag="VGP27_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HEV7851059.1"
                     /translation="MTDSRTPGLFTLVLHTHLPWLAHHGRWPVGEEWLYQSWSAAYLP
                     LMRVLRRLADEDRSHLLTLGMTPVVTAQLDDPYCLTGMHHWLANWRLRAQEATTLPEP
                     LRQFGVRE"
     gene            complement(327..1112)
                     /locus_tag="VGP27_06805"
     CDS             complement(327..1112)
                     /locus_tag="VGP27_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851060.1"
                     /translation="MSSFVTDGPAGPEGALPLTGERTIPGLAKENYWFRRHEVVYQRL
                     RDRCAGRDVLEAGCGEGYGADLIADVARRVIGLDYDEATVAHVRARYPRVDMRHGNLA
                     ELPLPDGSVDVVVNFQVIEHLWDQPQFVAECLRVLRPSGLLLMSTPNRVTFSPGRDTP
                     INPFHTRELNAAELTELLESAGFTMESVQGVFHGCPLAELDAKHGGSIIDAQVARAIA
                     DAPWPEDLLADVASVRIDDFELTDAADRDIDESLDLVAIAVRP"
     gene            1335..2126
                     /locus_tag="VGP27_06810"
     CDS             1335..2126
                     /locus_tag="VGP27_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV7851061.1"
                     /translation="MTNIVVLIKQVPDTWSERKLTDGDYTLDREAADAVLDEINERAV
                     EEALLIKERNGGESTVTVLTAGPQRATEAIRKALSMGADKAVHLLDEGLHGSDMVQTG
                     WALARALGTIEGTELVIAGNEATDGTGGAVPAIIAEYLGLPQLTHLRKVSVEDGKVTG
                     ERETDDGVFTVEASLPAVVSVTEKINEPRFPSFKGIMAAKKKEVTTLTLAEIGVDGDE
                     VGLANAGSKVVASTPKPPKTAGEKVTDEGDGGTKVAEYLVAQKII"
     gene            2169..3125
                     /locus_tag="VGP27_06815"
     CDS             2169..3125
                     /locus_tag="VGP27_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV7851062.1"
                     /translation="MAEVLVLVEHAEGALKKVTSELITAARVVGEPSAVVVGKPGTAD
                     PLIDGLKAAGAAKIYVAESDDAENYLITPYVDVLASLVESSSPAAVLLSSNADGKEIG
                     GRLAARTGSGVLSDVVEIKEGAKGIHSIFGGAFTVEAEATGDVPVITVRGGAIDAEPA
                     DGAGEVVTVDVPAQAENATKITKREPAVAGDRPELTEATVVVSGGRGVGSAENFSVIE
                     ELADSLGAAVGASRAAVDSGYYPGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTS
                     KTIIAVNKDEEAPIFEIADLGIVGDLFKVAPQLTEAVKARKG"
BASE COUNT          600 a         1076 c         1098 g          479 t
ORIGIN      
        1 ctcgcgaacg ccgaactgcc gcaacggttc cggcagcgtg gtggcctcct gggcgcgtag
       61 ccgccagttg gccagccagt gatgcatgcc cgtcaggcag tacgggtcgt ccagttgcgc
      121 ggtgacaacg ggtgtcatgc cgagggtcag gaggtggcta cggtcctcgt cggccagcct
      181 gcgcagcacc cgcatcagcg gcagatatgc cgccgaccac gactggtaga gccattcctc
      241 tccgaccggc cagcgaccgt gatgggccag ccacggcagg tgtgtgtgca gaacgagggt
      301 gaacagtccc ggcgtccgcg agtccgtcat ggccgcaccg cgattgcaac cagatcgagg
      361 ctttcgtcga tatcgcggtc ggccgcgtcg gtcagttcga aatcgtcgat gcggacggag
      421 gcgacgtcgg ccaacaggtc ctccggccat ggcgcgtcgg cgatcgcacg ggcgacctgc
      481 gcgtcgatga tcgagccgcc gtgcttggcg tccagctcgg ccagcgggca tccgtggaac
      541 acgccctgca cggactccat ggtgaacccg gcggactcga gcaactcggt cagttcggcg
      601 gcgttgagct cacgggtgtg gaaggggttg atcggcgtat cgcggccggg ggagaaggtc
      661 acccggttgg gcgtcgacat cagcagcagc cccgacggcc gcagcacacg caggcattcg
      721 gcgacgaact gtggctggtc ccaaagatgc tcgatcacct ggaagttcac cacgacgtca
      781 accgagccgt cgggcagcgg aagctccgcg agattgccgt ggcgcatgtc caccctcgga
      841 tagcgcgcac gcacatgggc caccgtggcc tcgtcgtagt ccagcccgat cacccggcgg
      901 gcgacgtcgg cgatcagatc ggcgccgtag ccctcgccgc agcccgcttc gagcacgtcg
      961 cgaccggcgc agcggtcacg caaccgctgg tagaccacct catggcggcg gaaccagtag
     1021 ttctccttgg ccagaccggg gatcgtgcgc tccccggtca gtggcagagc accttccggg
     1081 ccggcgggac cgtcggtgac aaatgagctc atcgcaaaag gcaggctaac ccaggagaag
     1141 ggttggtggc cggttcgcca acggttggcc cggttcttcg gcgagtaagg ggacatcaga
     1201 acacaaacct cgaccagcta tggtgttcct gcgaaccacc ttaagttacc gacgggtaac
     1261 atggtggcga ctgcttcaaa cgtggtagtg ggggccgacc gccggtcccc cttcgaggag
     1321 gacgaaccag actcatgacg aacatcgtgg tcctgatcaa acaggtccct gacacgtggt
     1381 cggaacgcaa gctgaccgac ggcgactaca cccttgaccg cgaggccgcc gacgccgtgc
     1441 tcgacgagat caacgaacgc gccgtcgaag aggcgctgct gatcaaggag cgcaacggcg
     1501 gcgagtcgac ggtgaccgtg ctgaccgccg gtccccagcg cgccaccgag gcgatccgca
     1561 aggcgctgtc gatgggtgcc gacaaggccg ttcatctgct cgacgagggc ctgcacggct
     1621 cggacatggt tcagaccggt tgggcgctgg cccgcgcact gggcacgatc gagggcaccg
     1681 agctggtcat cgccggtaac gaggccaccg acggcaccgg cggcgcagtg cccgcgatca
     1741 tcgccgagta cctcggcctt ccgcagctca cgcacctgcg caaggtgtcc gtcgaggacg
     1801 gcaaggtcac cggcgagcgc gaaaccgacg acggcgtgtt caccgtggag gcgtcactgc
     1861 ccgcggtggt gagcgtcacc gagaagatca acgaaccgcg cttcccgtcc ttcaagggca
     1921 tcatggccgc caagaagaag gaagtcacca cgctgaccct cgccgagatc ggggtggacg
     1981 gcgacgaagt cggtctcgcg aacgccggat cgaaggtcgt tgcgtcgacc cccaagccgc
     2041 cgaagacggc aggcgagaag gtcaccgacg aaggtgacgg cggcaccaag gtcgccgagt
     2101 acctcgtggc ccaaaagatc atctagcccc gctaccgacc acccctagac acagaggcga
     2161 aacaactcat ggctgaagta cttgtgctcg tcgagcacgc cgaaggtgcc ctcaagaagg
     2221 tcacctccga attgatcacc gccgctcgcg tggtgggtga accgtcggcc gtcgtggtcg
     2281 gcaagcccgg taccgccgac ccgctgatcg acggcctgaa ggccgccggc gcggccaaga
     2341 tctacgtcgc cgagtccgac gacgcggaga actacctgat caccccgtac gtcgacgtgc
     2401 tggcatcgct ggtggagtcg tcctccccgg ccgctgtgct gctgtcgtcc aacgccgacg
     2461 gcaaggagat cggcggtcgg ctcgccgccc ggaccggctc cggtgtgctc agcgacgtcg
     2521 tggaaatcaa ggagggcgcc aagggcatcc actccatctt cggtggggcg ttcaccgtcg
     2581 aggccgaagc caccggcgac gtgcccgtca tcacggtgcg cggcggtgcg atcgacgccg
     2641 agcctgccga cggtgctggc gaggtcgtca ccgtcgacgt gcccgcacag gccgagaacg
     2701 caacgaagat caccaagcgc gagcccgcgg tggccggcga tcgtccagag ctcaccgagg
     2761 ccacggtcgt ggtgtccggc ggccgcggcg tcggaagcgc cgagaacttc agtgttatcg
     2821 aggaattggc ggattcgctt ggtgcggccg tcggtgcgtc gcgtgcggcg gtggactccg
     2881 gctactaccc gggccagttc caggtgggcc agaccggcaa gaccgtttcg ccccagctgt
     2941 acatcgcact gggcatctcc ggcgcgatcc agcaccgcgc aggcatgcag acatcgaaga
     3001 cgatcatcgc ggtgaacaag gacgaagagg cgccgatctt cgagatcgcc gacctcggca
     3061 tcgtcgggga cctgttcaag gtcgccccgc aactcaccga ggcggtcaag gcccgcaagg
     3121 gctgacgccc cgcgcacttg ggcgcgtcca cgccgacttc accctcaacg tagggtagcc
     3181 gcgtggacgt gctgctcggc atcgatatcg gcaccggaag taccaagggc gtcctggtcg
     3241 atacctcggg caa
//
LOCUS       DASYLX010000280         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1108466,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000280 DASYLX010000000
VERSION     DASYLX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108466"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGP27_06820"
     CDS             <1..625
                     /locus_tag="VGP27_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEV7851063.1"
                     /translation="RKIQAKRGPEAFVSRHPLISLGALVLVIGFIFDAILEISLVRTG
                     LYIYSQAIPFGTLFGGSTFQFPLIWESLSVTFVMIPAAILVYRDDTGKSVAEKLAAKA
                     KLFPTKPVLGTFLVMFAIINVSYFAYGGWFWAIKASGLATSVACPWPFPEAKVYDPQG
                     YYEKAGAEGPYSVGKWSTWQQGLPDGRPDVQAPPPGQGACAPANANG"
     gene            618..1634
                     /locus_tag="VGP27_06825"
     CDS             618..1634
                     /locus_tag="VGP27_06825"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851064.1"
                     /translation="MGDRRTVVITGAARGLGFASAVRLYREGWRVVAAVRTPDRAMPL
                     LREATGARDDDDRLIGVQLDLMDAASISAAAKVIEERVGAPYALVHNAGISAAGMVEE
                     TDMQLWRNMFATHVMGPVALTKALLPAMREAGEGRIVLVCSSAGVRGQPGTGPYSASK
                     GAMERVGESLAGEIAPFGLGVTVLVTGTYDTDIITDAGTTDDRDFAGPYARLHQTMNS
                     RGRYAIKFARPPQRFADGLVNALADRGAFRRHGVGPDASMLLVSNRVLPSAGMHHMSR
                     IVLGIPRQGAMRGGAYPLTTAQRAIVFAAKVLPQPVLQRLASLAVRFSPAKQAPPQGN
                     ENDA"
     gene            1627..3111
                     /locus_tag="VGP27_06830"
     CDS             1627..3111
                     /locus_tag="VGP27_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7851065.1"
                     /translation="MPDTQKVDFESRMMIDGKLVDGEAGTFTNINPANEDVLGEVADA
                     SKADMHRAIDAARRSFDETDWSTNHELRKRCLEQLHEAIESELEEVREELILEVGAPR
                     AVTHGPHLDAPLEDGLKYPARLIDSFEWEIDLGDKVVSVTGVNTRRKVWHEPVGVVGA
                     IVPWNFPFEVTINKLGQALATGNTVVLKPAPNTPYNATRLGRLIAEKTDIPAGVVSVV
                     TASDHFVGEELTLSPKVDMISFTGSTVVGKRIMEKGAATMKRLFLELGGKSATIVLED
                     ADFNTACLIGIGPLMHAGQGCAAPTRMLLPRSRYDEGVAILKGIYENIPAGDPQDPGT
                     ICGPVISAKQQSRILGYVKKGVDEGATMLVGSTEAAKQFDKGFWVNPTLFTDVDNSMT
                     IAQEEIFGPVLVVIPYEDEEDAIRIANDSVYGLAGNVMSSSLEHSLAVARRLRAGFIG
                     LNGTAGYGADTPFGGYKDSGVGRQNGLAGFSQYTEVKSVAYPAF"
     gene            3156..>4375
                     /locus_tag="VGP27_06835"
     CDS             3156..>4375
                     /locus_tag="VGP27_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851066.1"
                     /translation="MSDLFDDLEDFGAFDDVVSGNVRDPYPELAKQRQETPIQRIDMS
                     AMPGEEGKPVFIVYRHEDIQQMLRDNETYSSNVVIQIFGDVLGHGVMLGMDEPLHGRL
                     RSLVTKAFTQKALARWEDQIVGRIGNELIDQFASDGKTDLVKTFTFPYPTRIIAALLG
                     LPEEDFPQFQRWSISMLSFTVNPERGKAASQALVDYFKPILQARRAEPREDLISSLAA
                     AEIDGHKLDDEDIYSFIRLLLPAGVETTYRSLGNLLFGLLTNQDQLDAIRADRSLIPQ
                     AIEEAVRWDAPLLNITRVATRDTELSGVPIPQGSSVMPMLGAANRQEDRYPEPDRFDI
                     FHQAKVPISWGHGVHVCLGMHLARLEMRTAVNLLLDRLPNLRLDPDGDDPHIRGMVFR
                     SPTSLPVLFDPSTR"
BASE COUNT          803 a         1379 c         1445 g          748 t
ORIGIN      
        1 tcgcaagata caggcgaagc gcggcccgga agcgttcgtc tcccggcacc cgctcatcag
       61 cttgggtgcc ctcgtgctgg tgatcggctt catcttcgac gccattctgg agatcagctt
      121 ggtgcgcacg gggctctaca tttactcgca ggcgatacct ttcggaacgt tgttcggtgg
      181 cagcaccttc cagtttccgc tgatctggga atcgctgtcg gtgaccttcg tgatgatccc
      241 ggcggccatc ctcgtctacc gcgacgacac ggggaagtcg gtggcggaga agctcgccgc
      301 gaaggccaag ttgtttccca ccaagccggt gctcggcacg ttcctggtga tgttcgcgat
      361 catcaacgtg tcgtacttcg cctacggcgg ctggttctgg gctatcaagg ccagcgggtt
      421 ggccacatcg gtcgcctgcc cgtggccgtt ccccgaggcc aaggtttacg acccgcaggg
      481 ctactacgag aaggccggtg ccgagggacc gtactcggtc ggcaagtggt ccacctggca
      541 gcaaggtctg ccggacggtc gccccgacgt ccaggcgccg ccgccaggtc aaggagcctg
      601 cgcgcctgcg aacgcgaatg ggtgaccgca ggacggtggt catcaccggc gcggcgcgtg
      661 gactcgggtt cgcatcggcg gtgcggctct atcgcgaggg ctggcgcgtg gtcgccgccg
      721 tgcgcacgcc cgaccgcgcc atgccgttgc tgcgcgaggc gaccggagcg agagacgacg
      781 acgaccggct gatcggcgtc cagctcgatc tgatggacgc agcgtcgatc tcggccgccg
      841 caaaggtaat cgaggagcgc gtcggcgcgc cgtacgcgct cgtgcacaac gccggaatct
      901 ccgccgcggg aatggtggag gaaaccgata tgcagttgtg gcggaacatg ttcgctaccc
      961 acgtgatggg tccggtggcg ctgaccaagg cgttgttgcc cgccatgcgg gaggcgggcg
     1021 agggccggat cgtgctggtg tgcagttcgg ccggagtgcg cgggcagccg ggcacaggtc
     1081 cctactcggc gagcaagggc gccatggaac gcgtgggcga atccctggcg ggtgagatcg
     1141 ccccgttcgg gctgggggtg acggttctgg tcaccggcac ctatgacacc gacatcatca
     1201 ccgatgcggg caccaccgac gaccgggatt tcgcggggcc gtacgcgcgg ctgcaccaga
     1261 cgatgaacag ccgcggccgg tacgcgatca aattcgcccg gccgccgcaa cggttcgccg
     1321 acggtctggt gaatgcgctg gcagaccgcg gagccttccg ccgtcacggc gttggaccgg
     1381 acgcgtcgat gttgttggtg tccaacagag tgctgccctc tgcggggatg catcacatgt
     1441 cccggatcgt gctgggcatt cccagacagg gcgcgatgag gggtggggcc tacccgttga
     1501 cgactgcgca gcgggcgatc gtgttcgccg ccaaggtgct tccgcaaccg gtcttgcagc
     1561 gcctggcgtc gttggcagtg cggttttcac cggccaagca agcgccacca caggggaatg
     1621 agaacgatgc ctgacactca gaaggtcgac ttcgagtcga ggatgatgat cgacggcaag
     1681 ctcgtcgacg gtgaagcggg gacgttcacc aacatcaacc cggcgaacga agacgttctc
     1741 ggcgaggtcg ccgatgcgtc gaaggccgat atgcaccggg ccatcgacgc cgcccgccgg
     1801 tcgttcgacg agaccgactg gtcgacgaac catgaactgc gcaagcggtg cctcgagcaa
     1861 ctgcatgagg cgatcgagag cgaactggag gaagtccgcg aggaactcat cctcgaggtc
     1921 ggggctccca gagccgtgac acacggcccg cacctggatg ctcccctcga agacggcctc
     1981 aaataccctg cgcgactgat cgactcgttc gagtgggaaa tcgacctggg cgacaaagtg
     2041 gtatcggtga ccggtgtcaa caccaggcgc aaggtgtggc atgagccggt tggggtggtc
     2101 ggcgcgatcg tgccgtggaa ctttccgttc gaagtcacca tcaacaagct gggacaggcg
     2161 ctggccactg gcaacaccgt ggtgctcaag ccggcgccga ataccccgta taacgcgacg
     2221 cggctcggac ggctcatcgc cgagaagacc gacatcccag caggtgtcgt cagcgttgta
     2281 accgcatcgg accacttcgt cggcgaagag ctcacgctgt caccgaaggt cgacatgatc
     2341 tcgttcaccg gctcgaccgt cgtgggcaag cgaatcatgg aaaagggcgc cgcgacgatg
     2401 aagcggctgt tcctcgagct cggcggcaag tccgcgacga tcgtgttgga ggatgccgac
     2461 ttcaacaccg cctgtctcat cggcatcggg ccgctgatgc atgccgggca ggggtgcgcc
     2521 gcacccaccc gaatgctctt gccgcgctcc cgatacgacg agggcgttgc catcctgaag
     2581 ggcatctatg agaacattcc tgccggtgat ccacaggatc ccggcaccat ctgcggtccc
     2641 gttatctccg ccaagcagca gtcccgcatc ctcggctacg tcaaaaaggg tgtcgacgag
     2701 ggcgcaacga tgctggtcgg aagcacggag gcggccaagc agttcgacaa gggattctgg
     2761 gtcaacccaa cgcttttcac cgacgtcgac aactcgatga cgatcgccca ggaagagatc
     2821 ttcggacccg tcctggtcgt cattccctac gaagacgaag aagacgccat ccggatcgcc
     2881 aacgacagcg tgtacggcct cgcaggcaat gtgatgtcga gctcgctgga gcattcgctt
     2941 gccgtagcgc gacggctgcg cgcgggattc atcgggctca acggcacggc gggctacggc
     3001 gcggacacgc cgttcggcgg ctacaaggac agtggcgtcg gccgccagaa cggcctcgcg
     3061 gggttcagcc agtacaccga agtgaaatcc gtggcctacc cggccttctg acgagcgctt
     3121 gcgcgaggaa tattcagccc agataggagc caacaatgtc agatctgttc gacgacctcg
     3181 aagatttcgg ggcgttcgac gacgtcgtct cgggcaatgt gcgtgatccc tacccggagt
     3241 tggccaaaca gcgccaggag acgccgattc agcggatcga catgtccgcg atgccgggcg
     3301 aggagggcaa gcccgtcttc atcgtgtacc ggcacgagga catccagcag atgttgcgcg
     3361 acaacgagac ctactcgtcc aacgtcgtga tccagatctt cggcgacgta ctcggccacg
     3421 gggtgatgct gggcatggac gagcccttgc acggccggct gcggtcgctg gtgacgaagg
     3481 cgttcaccca gaaggcgctc gcgcgctggg aggaccagat cgtcgggcgc atcggcaacg
     3541 aactgatcga tcagttcgcc tcagacggca agacggactt ggtcaagaca ttcacctttc
     3601 cgtacccaac ccgcatcatc gccgcgctgc tgggcctgcc ggaggaggac ttcccgcagt
     3661 tccagcgctg gtctatctcg atgctcagct tcaccgtcaa cccggaacgt ggtaaggcgg
     3721 cgtcgcaggc gttggtggac tacttcaagc cgatcctgca ggcccgtcga gccgagccac
     3781 gtgaggacct gatcagcagc ctcgcagcgg ccgagatcga cgggcacaag ctggacgacg
     3841 aggacatcta ctcgttcatc cggctgctgc tccccgccgg ggtggagacg acgtaccggt
     3901 cgctgggcaa cctgttgttc gggctgctca ccaaccaaga tcaactcgat gcgatccgag
     3961 ccgaccggtc tctcataccg caggcgatcg aggaggcggt gcggtgggac gcgccgttgt
     4021 tgaacatcac cagggtcgcg acccgtgaca ccgagctgtc cggggtgccg attccgcagg
     4081 gctcgtcggt gatgccgatg cttggtgccg ccaaccggca ggaggaccgg tatcccgagc
     4141 cagaccgctt cgacatcttc catcaggcca aggtgccgat cagctggggc cacggcgttc
     4201 acgtctgtct cggcatgcac ctggctcggc tcgaaatgcg cactgcggta aaccttctgc
     4261 tcgaccgatt gcccaatctg cggctggatc ccgacggcga cgacccgcat atccgcggca
     4321 tggtgtttcg ctcgccgacg tcgctaccgg tgctcttcga cccttcgacc aggag
//
LOCUS       DASYLX010000281         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3850888,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000281 DASYLX010000000
VERSION     DASYLX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850888"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /gene="purB"
                     /locus_tag="VGP27_06840"
     CDS             complement(<1..951)
                     /gene="purB"
                     /locus_tag="VGP27_06840"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV7851067.1"
                     /translation="MAIPNVLASRYASDEMVAIWSQEAKIIGERRLWLAVLRAQAELG
                     VSVPDGVVADYERVIEDVDLASIAERERVLRHDVKARIEEFNALAGHEHVHKGMTSRD
                     LTENVEQLQIRRSLELVFSHGIAVVARLAERAVSYRDLVMTGRSHNVAAQATTLGKRF
                     ASAAEEMLIALSRLRELIDRYPLRGIKGPMGTAQDMLDLFDGDTSRLADLERRVADFL
                     GFTEVFTSVGQVYPRSLDHDVVSALVQIGAGPSSLAHTIRLMAGNELVTEGFAPGQVG
                     SSAMPHKMNTRSCERVNGLQVVLRGYASMVAELAGAQWNEG"
     gene            1005..1862
                     /locus_tag="VGP27_06845"
     CDS             1005..1862
                     /locus_tag="VGP27_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEV7851068.1"
                     /translation="MHFVGLDLAWGEKNQTGVAAVDADGRLLHVGAAHDDANIIDAIA
                     PFTAGECLVAFDAPLIVNNQTGHRPAEAAFNRDFAKFDAGARPAFAEKPELRHPRAAR
                     LAAALDLDMDPASGASRRAIEVYPHPATVVLFELDKTLKYKRGPFGDRQRDLLKLMTL
                     IEGLDDATPRLRANRSVAWVELRKRVEAATKPVQLDRDEDPVDAVLCAYVGLYWYCRP
                     DEVTIYGDFETGYIVTPSLPPDLSPAPRRRVVPPPSDELHERLSRLEDLLAQAQSEAK
                     TIRERLYRM"
     gene            complement(1863..3287)
                     /locus_tag="VGP27_06850"
     CDS             complement(1863..3287)
                     /locus_tag="VGP27_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7851069.1"
                     /translation="MSQTDTVDQQPELKRVMGPGLLLLFVVGDILGTGVYALTGDVAA
                     EVGGAAWVPFLVAFLIATVTAFSYLELVTKYPQAAGAALYAHKAFGIQFVTFLIAFVV
                     MCSGITSASTAARTFASNFFKGFDTSWGTLGVVALALFFMAALALINLRGVGESVKLN
                     VLLTVVEISGLVLVILVGMWAFTGGGDVDFSRVVAFDTAEDKNAFMAITAATSLAFFA
                     MVGFEDSVNMAEETKDPVRIFPKMLLTGLSIAGIVYILVSIVAVALVPVGELEASDTP
                     LVEVVKAGAPGLPIDKILPFITMFAVSNTALINMLMASRLIYGMARQHVLPPVLGKVH
                     PQTRSPWVAILFTTAIAFGLIIYVTAFANDDAISVLGGTTSLLLLAVFAVVNVAVLVL
                     RRDVRETGGHFKTPTLLPVIGCIASLYLVTPLSGRPSQQYVLAGVLVAIGIVLFLVTR
                     VINRQLGVRDAGITDPTQLAEAPD"
     gene            complement(3388..>3684)
                     /locus_tag="VGP27_06855"
     CDS             complement(3388..>3684)
                     /locus_tag="VGP27_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851070.1"
                     /translation="YERYIACARQPALREVQRRILQQRTDAVVKVVERSGRSVRAELV
                     PALVCAVDGAVVAALVDDNNGPRSAARATLIDVIDVLAPITAPISVPVLTAASG"
BASE COUNT          711 a         1266 c         1138 g          569 t
ORIGIN      
        1 gccctcattc cattgcgccc cagcgagttc ggccaccatc gacgcatagc cgcgcaacac
       61 cacctgcagc ccgttcactc gttcgcacga tcgggtgttc atcttgtgcg gcatcgccga
      121 cgagccgacc tgaccgggcg cgaacccctc tgtcaccaac tcgtttcccg ccatcaaccg
      181 gatggtgtgc gccagcgacg acggccccgc gccgatctgc accagcgctg aaaccacgtc
      241 gtggtcaagc gatcgcgggt agacctggcc gacgctggtg aagacttcgg tgaagcccag
      301 gaaatcggct acgcgacgct cgagatcagc aagccgcgac gtgtcgccgt cgaacagatc
      361 gagcatgtcc tgcgccgtgc ccatcggccc cttgatgccg cgcaacggat accggtcgat
      421 cagctcacgc agtcgggaca gtgcgatcag catctcctcg gccgccgacg cgaatcgctt
      481 gcccagcgtc gtcgcctgcg ccgcgacgtt gtggctgcgt ccggtcatca ccagatcgcg
      541 gtagctcacc gcgcgctcag ccagccgcgc gaccaccgcg atcccatgcg agaacaccag
      601 ctccagagac cgccggatct gcagctgctc aacgttttcc gtgaggtcac ggctcgtcat
      661 gcccttgtgc acgtgctcgt gacccgcgag tgcgttgaac tcctcgattc gggccttgac
      721 gtcatggcgc agcacccgct cccgctccgc gatcgaggcc aggtcgacgt cttcgatcac
      781 ccgctcgtag tcggccacca caccgtcggg aacggagacg cccaactcgg cctgtgcgcg
      841 cagcaccgcc agccatagtc ggcgctcgcc gatgatcttg gcctcctgcg accagatcgc
      901 caccatctcg tcgctggcgt acctgctggc cagcacgttg ggaatcgcca caaatacaca
      961 gcttacggcg gcgagtaggc gcacgtttga ggcacagtag ccctatgcac ttcgttgggc
     1021 tcgatctcgc ttggggagag aaaaaccaga ccggtgtcgc ggccgtcgac gccgatggtc
     1081 gactcctaca cgtgggtgcc gcccacgacg acgccaacat catcgacgcc atcgccccgt
     1141 tcaccgccgg cgaatgccta gtcgccttcg atgccccgct gatcgtcaac aaccagaccg
     1201 ggcatcgacc cgccgaggcc gcgttcaacc gcgacttcgc aaagttcgac gcgggggcac
     1261 gcccggcgtt cgccgaaaag cctgagctga ggcacccacg cgccgcccgg ttggcggccg
     1321 cactcgatct cgacatggac ccggcctctg gtgcgagccg acgggcgatc gaggtctatc
     1381 cgcatccggc gaccgtcgtg ctgttcgagc tcgacaagac actgaagtac aagcggggcc
     1441 cgttcggcga ccgccaacga gatctgctga agttgatgac gctgatcgag ggactcgacg
     1501 acgccacacc gcgattgcgc gcgaaccgca gcgtcgcctg ggtcgaactg cgcaagcgcg
     1561 tcgaggcggc gacgaaaccc gtgcagctcg accgggacga ggatccggtc gacgcggtgc
     1621 tctgcgccta cgtcggcctg tactggtact gccgccccga cgaggtgacg atctacggcg
     1681 atttcgagac gggctacatc gtcaccccga gccttccccc ggacctgtcc cccgcgccgc
     1741 gaaggcgggt ggtgccgccg ccgagcgacg aactacacga acgcctttcg cgtctggaag
     1801 atctgctggc gcaggcgcaa tcggaggcga agacgatccg cgaacgcctc taccggatgt
     1861 gactagtcgg gtgcttcggc cagctgcgtc gggtcggtga tgcccgcgtc acgcaccccg
     1921 agctgacggt tgatcacccg cgtgacaaga aacagcacga tgccgatggc gaccagaaca
     1981 ccggccagca cgtattgctg cgacggccgc cccgaaagcg gtgtcacgag atacagcgac
     2041 gcgatgcacc cgatcacggg cagcagggtc ggcgtcttga agtgcccacc tgtctcgcgc
     2101 acgtcgcggc gcagcaccag gacggcgaca ttcaccaccg cgaacaccgc caacagcagc
     2161 agcgacgtgg ttccgccgag caccgagatc gcgtcgtcgt tggcgaacgc cgtgacatag
     2221 atgatgaggc cgaaggcgat tgcggtcgtg aaaaggatcg cgacccaggg gctacgggtc
     2281 tgcggatgga cctttcccag caccggcggc agaacgtgct ggcgcgccat gccgtagatg
     2341 agccggctgg ccatcagcat gttgatcaac gctgtgttcg acaccgcgaa catcgtgatg
     2401 aacgggagaa tcttgtcgat cggcaagccc ggcgcaccgg ctttgacgac ctccacgagc
     2461 ggcgtgtcgc tggcctccaa ctcgccgacc ggcacgagtg cgacggcgac gatcgacaca
     2521 aggatgtaga cgatgccggc aatggacagg ccggtgagca gcatcttggg aaagatccgg
     2581 accggatcct tggtctcctc ggccatattc accgagtcct cgaaaccgac catcgcgaag
     2641 aaagcaagcg atgtggccgc tgtgatggcc atgaaagcgt tcttgtcctc ggcggtgtcg
     2701 aacgcgacca cgcgggagaa gtccacatcg ccgccgccgg tgaacgccca catgccgacg
     2761 agaatgacca gaaccagacc gctgatctcg acgaccgtca gcaacacgtt gagcttgacg
     2821 ctttctccga ccccacgcag gttgatgagc gccagcgcgg ccatgaagaa cagggcgagt
     2881 gcgaccaccc cgagcgtgcc ccagctggtg tcgaaaccct tgaagaagtt cgaggcaaat
     2941 gtcctggccg ccgtcgacgc cgaggtgata cccgagcaca tcaccacgaa cgcgatcaaa
     3001 aacgtcacga actgaatgcc aaaagccttg tgcgcgtaga gcgcagcgcc tgcggcctgg
     3061 gggtatttgg tgacgagctc caaatagctg aacgcggtga cggtcgcgat gagaaaggcc
     3121 accaggaacg gcacccatgc ggcgccaccg acttcggcgg ccacgtctcc ggtcagcgca
     3181 tacacacccg tgccgagaat gtcgccgacg acgaacaaca gcagcagccc cggccccatc
     3241 acccgcttca gttcaggttg ctgatcgacg gtgtccgtct ggctcatggg gcctccctgt
     3301 cagtcggctg tgagtacccc atcatcgacc ggcagcaaac gcgccgcacc cgcaacggcg
     3361 caaagaagac ttcgaccgtg accggggtca gcccgaagcc gcggtgagca cgggtaccga
     3421 gatcggcgcc gtgatcggtg cgagcacgtc gatgacgtcg atcaacgtgg cgcgggcggc
     3481 acttcggggg ccgttgttgt cgtcgacgag cgccgctacc actgcgccgt cgaccgcaca
     3541 caccagtgct gggacgagct ctgcgcgcac cgaccgtccg gaccgctcga cgaccttgac
     3601 cacggcatcg gttcgctgct gcaggatccg ccgctgcacc tcgcgcagag ccggctgacg
     3661 cgcgcacgcg atgtagcgtt cgta
//
LOCUS       DASYLX010000282         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1108516,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000282 DASYLX010000000
VERSION     DASYLX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108516"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGP27_06860"
     CDS             <1..219
                     /locus_tag="VGP27_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV7851071.1"
                     /translation="RTRATAWQVATGYGQRSLIETTMGRYKTLTGPRLRARGLAAQQT
                     EAAVSVAVLNRMLAAGRPDSVRRQPPIA"
     gene            complement(459..707)
                     /locus_tag="VGP27_06865"
     CDS             complement(459..707)
                     /locus_tag="VGP27_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851072.1"
                     /translation="MEKNTPLGVEMAARLLSLEDFAMTARIVVPVTPDLIKEVFNKAP
                     ALRRAEADVLARDPRFAIGIYRAAVAMGAMDSIRFKRK"
     gene            complement(715..1107)
                     /locus_tag="VGP27_06870"
     CDS             complement(715..1107)
                     /locus_tag="VGP27_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851073.1"
                     /translation="MIRTEILTRYRRLRQISKEHHQAVLDIIAPDVVLNWAKRLDLTQ
                     GRTVLLESEHEMTLVEDLAIYLPRLGRSHPLDRYARAAQFASGSGEAIVLEAMRQARF
                     SLWRVERRHENRGSGPARSAARRGDPAS"
     gene            1333..2670
                     /locus_tag="VGP27_06875"
     CDS             1333..2670
                     /locus_tag="VGP27_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV7851074.1"
                     /translation="MFTQPSRLNAVALATGALLCLNRRTVCGALRAVDGASDTGFSRF
                     HRFLSRAAWSGLTGSRILLGLLLKAFVGDGEPMVVAVDDTVERRRGERIRAKGIYRDP
                     VRSSRGFFVKVEGLRWLSFQLAAKPGFASRIWGLPFLTVLCRSERANAKRKRRHQTVP
                     EKAAWGMRLIARWCPGRLLVMVGDGAFASLELFRALRGDAVCVARCRMDARFFNPPTP
                     RKNGQKGRPRVVGTRQLTPKTRSIRKTTQWVRMIIPGWRAEDGMTGRDVDVATGTALW
                     NSRGITLPVRWVLTRDPAGRAETRTFVCSEPRRSASEILTWYAMRWASEVTFEEARRH
                     LGVETQRQWSDLAIHRTTPLLFGLFSLVTLWASELAAKTGKLPVLGAAWYKKPNPTFS
                     DCLAAVRRILWAEEAVCPILWRDDFPTWRSQARTAEKPRPLQKRLAELMSYAA"
BASE COUNT          492 a          852 c          983 g          546 t
ORIGIN      
        1 cgaacaaggg cgactgcctg gcaggtcgcg accggttatg gccagcgatc gttgatcgaa
       61 acaacaatgg gccgctacaa gacgctgacc gggcctcggt tgcgcgcccg cgggctcgcc
      121 gcccaacaga ctgaggctgc cgtcagcgtg gcggtgctga accggatgtt ggcggccgga
      181 cgcccggatt ccgttcgtcg ccagccgcct atcgcatagt agcctgggtt tggggcatct
      241 cgcctttggt ccatcgagtg caccaacgcc gctccgcttt actgaggatc cggtgctcgc
      301 gaccccacca ggcagataac ggccatctac tcgactgcga ttgattaagt ttatgggcgg
      361 gtgtcagatg ctgtctaaaa tgaagggaga cgctcatctg gagtttggca cttttggatg
      421 ggtgagggag atcacgttcg tatccatgaa gctggttgct atttgcgctt gaagcggatg
      481 gagtccatcg ctcccatggc caccgcggct cggtagatgc cgatcgcgaa gcgtgggtcc
      541 cgggccagga cgtccgcctc ggcgcgccgc aaggcgggag ccttgttgaa gacctccttt
      601 atcagatccg gggtgaccgg cacgacgatg cgggcggtca ttgcgaagtc ttcgagactt
      661 aggagacgcg cggccatctc cacccctaat ggcgtgttct tttccatggc ctcgtcaact
      721 agccggatct ccccgccgcg cagcagatct cgcaggacca gacccgcggt tttcgtgccg
      781 ccgttcgaca cgccagaggg aaaaacgggc ctgacgcatc gcctcgagca cgatcgcctc
      841 gccggagccg gaagcaaact gtgcggcccg ggcatagcgg tcgagcgggt gtgagcggcc
      901 cagtctgggc aggtagatgg caagatcctc cacgagcgtc atctcatgtt cgctttcgag
      961 cagcaccgtc ctgccctgcg tgaggtcgag ccgcttggcc cagttcagca cgacgtctgg
     1021 cgcgatgatg tccaggacag cctggtggtg ttccttgctg atctgacgca ggcggcggta
     1081 acgggtcagg atctcggtcc ggatcatcgg tagcggcctt cagcggctcg gggtggcaga
     1141 ttgccatcgt gcaagctgcg gcggaagcaa gctggccagg gcgagcgcag acgggacact
     1201 ggcggcgacc cggccccctc cgctccctca agacgaggta ctgtatgcct catgggagag
     1261 taagccatga cgcagagtgg tgaaaccggc agcgcgcggg cgatggaact ctggctgttg
     1321 ccgttcgcgg cgatgttcac gcagccatcg cggctaaacg cggtcgcgct ggcgacggga
     1381 gcgttgctat gcctgaaccg ccggacggtg tgcggggcgc tgcgggcggt agacggtgcc
     1441 tcggacacag gtttcagtcg atttcatcgg ttcttgtcgc gcgcggcgtg gtcgggcctg
     1501 acaggctcga ggatcctgct tggcttgctg ttgaaggcgt tcgtagggga cggcgagccg
     1561 atggtggttg ccgtggacga caccgtcgaa cgtcgacgcg gagagcggat ccgcgccaaa
     1621 gggatctacc gtgatccggt gcggtcgagc cgcgggttct tcgtcaaggt tgagggcttg
     1681 cgatggctgt cgttccagtt ggcggccaag cccggtttcg ccagccgcat ctgggggttg
     1741 ccgttcctga cggtgctgtg ccgttcggaa cgggccaatg cgaagaggaa gcggcggcac
     1801 cagacggtgc cggagaaggc cgcctggggg atgcggctga tcgcccgctg gtgtccgggg
     1861 cggctgctcg tgatggtggg ggacggtgct ttcgccagcc tggagctttt ccgcgcgctg
     1921 cgtggcgatg ccgtgtgcgt cgcccgctgc cggatggacg cgcgcttttt caacccgccg
     1981 acgccgcgca agaatgggca gaagggccgg ccgcgtgtcg tcggaacccg tcaactgaca
     2041 ccaaagacgc ggtcgatcag gaagaccacc cagtgggtac gcatgatcat tccgggatgg
     2101 cgagccgagg atggcatgac gggtcgggac gtcgatgtcg cgaccggaac ggcgctgtgg
     2161 aattcacgtg gcatcacctt gccggtgcgc tgggtattga cccgggatcc ggctggacgc
     2221 gccgagacgc gcactttcgt ctgctccgag ccgcggaggt cggcatcgga gatcctgaca
     2281 tggtatgcga tgcgatgggc gagtgaggtt accttcgagg aggcccggcg ccatctcggg
     2341 gtcgagacac agcgccaatg gtcggacctg gcgatccacc gaaccacgcc gctgctgttc
     2401 ggactcttct ctctggtgac gctgtgggcc tccgaactgg ccgcgaagac cgggaagcta
     2461 ccggtgctcg gcgctgcttg gtacaagaag ccgaacccga cattctcgga ctgccttgcc
     2521 gccgtcaggc ggatcctctg ggcagaggag gccgtgtgcc cgatcctgtg gcgggacgat
     2581 tttccaactt ggcggtcgca agcacgaacc gccgaaaaac ccaggcccct tcagaagcgt
     2641 ctggcggagc ttatgtccta cgctgcttga caccctcaga aaccgccaaa gtccagctaa
     2701 gtgcgtgcag caggaccggt atctcgaagt tgcttccaaa ctcatgagcc aaccgggcca
     2761 gcgataagtg ctcatgtcgt tcgcatcgcc ggcatccgat ctcaagctcg agcaggtgtt
     2821 gtgcaacgtc tctgagtgtt atcatgtttg tagctgacta tttatgagcc gcc
//
LOCUS       DASYLX010000283         4040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_11754, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000283 DASYLX010000000
VERSION     DASYLX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11754"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VGP27_06880"
     CDS             complement(<1..632)
                     /locus_tag="VGP27_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851075.1"
                     /translation="MSTPARAIAPRIQRTARNWADGWNKVGEQTQFFGKTIVGIKDVW
                     IYYRVELLRQIAQMGLGVGAMALIGGTIVVVVFLTSQVGSLVAVQLYGSLANIGVEAL
                     SGFASAYINTRFAAPAVVGIAMAATIGAGCTAQLGAMRINEEIDALEVISVRTVSYLC
                     STRLVAGVVLAIPLYCLGLMAAYLATRFGTVLGYGQSTGVYDHYFDTFLNP"
     gene            complement(629..1339)
                     /locus_tag="VGP27_06885"
     CDS             complement(629..1339)
                     /locus_tag="VGP27_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851076.1"
                     /translation="MSMDAMVQMFKPNFPFREFVLQTWFVARVSVLPLILMAIPFVVL
                     TAFILNLLLIEIGAADASGAAAALGAVLYIGPIITVLVISGAGATAMCADLGSRTIRE
                     EIDAMRVLGINPIHRLVVPRVLAATLVATMLTSLVIVVGLTFGFFFSVFLQGVTPGAF
                     VASLTLLVGIPELIITLCKAILFGFFAGLIACYKGLSVGGGPQGVGTAVNETVVFTFL
                     VLFLIGTITTIVGVKATL"
     gene            2558..3553
                     /locus_tag="VGP27_06890"
     CDS             2558..3553
                     /locus_tag="VGP27_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7851077.1"
                     /translation="MKWGLPWPGQQLAAAAEAAGASAFCAGEFADLSAYVTATEMAHA
                     TSTAMIGPGIAYAFARSPFVHAASVRHLSKHAAGRVFLGLGAGTSRMNRDWFGVESAH
                     PAPRMAELIDVIRAFLHAENGEPIRYDGDFYSIDADIRAPVFGRLDVPILIGAFNKIM
                     IRTAGRTADGVLGHGLFTDRWWEEVVDPELARGADGAGREPGDLRRWGWLITAIDDAD
                     PKRAVAEARLQIAFYLTVRTYDSLVELHGWQDEVAAIRADFRNGRPKAMADHVTDDML
                     WAVAICGDAAQARDMLAHRKRLPDTAFFGAPSFLVGRRRRAEYDAAAVRLASELH"
     gene            3630..>4040
                     /locus_tag="VGP27_06895"
     CDS             3630..>4040
                     /locus_tag="VGP27_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7851078.1"
                     /translation="MSRPESLAPMISPEAASGAVRSPKTAELVARTLRRMVVEGQLKD
                     GDFLPHEAELITHFQVSRPTLREAVRVLESERLVEVRRGSRTGAKVRVPGAEIVARPA
                     GLLLALSGATLADVMTARIAIEPAAAKLLAEDGTA"
BASE COUNT          807 a         1288 c         1250 g          695 t
ORIGIN      
        1 gggttcaaaa aagtgtcgaa gtagtggtcg tagacgcccg tcgactgccc gtaaccaaga
       61 actgtgccga agcgggtcgc gaggtaggcg gccatcagcc cgaggcagta cagcgggatc
      121 gccagcacga ctccggccac cagccgcgtc gagcagaggt agctgactgt gcggacactg
      181 atgacttcga gggcgtcgat ctcctcgttg atccgcatcg cgcccagctg cgccgtgcag
      241 ccggcaccga tcgtggcggc catcgcgatc ccgaccacgg cgggagcggc gaagcgggtg
      301 ttgatgtacg cagatgcgaa gccgctcagc gcctccaccc cgatgttggc cagtgaaccg
      361 tagagctgta cggcgaccag tgagccgacc tgcgaggtca ggaagacgac gaccacaatg
      421 gtgccgccga tgagagccat cgctccgacg cccaggccca tctgagcgat ctggcgcagc
      481 agctccaccc tgtaatagat ccagacatcc ttgatgccga caatcgtctt gccgaagaat
      541 tgggtctgct cgccgacctt gttccagccg tcggcccaat tccgggcggt gcgttgaatc
      601 cgcggcgcga tggcgcgcgc tggggtgctc acagggtcgc cttcacgccg acgatcgtcg
      661 tgatcgtgcc gatgaggaac agaaccagga acgtgaaaac gacagtctcg ttgacggcgg
      721 taccgacgcc ctgcgggccg ccgcccaccg agagcccctt gtaacaggcg atcaggccgg
      781 cgaagaagcc gaacaagatt gccttgcaca gggtgatgat caactcgggt attccgacca
      841 gcagggtcag gctggcgacg aaggcgccgg gcgtgacgcc ctgaagaaag accgagaaaa
      901 agaagccgaa ggtcaagccc accacaatca ccaacgacgt cagcatggtc gcgacgagcg
      961 ttgctgccag cactcgcggc accaccaacc ggtgaatcgg attgatgccg agtacacgca
     1021 tcgcgtcgat ctcttcacga atggtgcgcg aaccgaggtc ggcgcacata gccgtcgccc
     1081 cggcgcccga gatcaccagc accgtgatga tgggaccgat atagaggacc gcacccagtg
     1141 cagccgcggc gccggatgcg tcggccgcgc cgatctcgat caggagcagg ttgaggatga
     1201 acgcggtcaa caccacgaat gggatcgcca tcaggatgag cggtaggacg gatacccgcg
     1261 caacgaacca ggtctgcagg acgaactcac ggaaggggaa gttcggcttg aacatctgga
     1321 ccattgcgtc catcgacatt gcgaagaagt cgccgacgga ccgcagcggc ttggctacat
     1381 cccactgctt gtgtcctgcc actagcggat ctccccgtcc ccttcggcag gattagcagc
     1441 cccggcggtg gcacacttca tagactgcga atcccctcca tcaagctcgg cgactgcccc
     1501 gcccagagtg attcaaggca caggcgactg ccgacaaacc gtacaccaac tttttcggcc
     1561 atagcaacaa ctcggcggca tttataacat taacggctca gttcacctag ttcgataggt
     1621 caggtttgct ctgcttgtct gcgacagatc cgcggggaac gtctcaccgg tcgataccag
     1681 cgaaaagtgt ctgccgttga gccaaggacc gtgtcgctcg acaactcagc cgtttgacca
     1741 tcagtacagt tgagagcact ggcattgcgc gattcgacgc gtgaccaacg acctcggacc
     1801 acatgtacgc accctggcga actacaggcg tcgaccgcca catgttgtcg cttggtgttt
     1861 gctgactgtt tcgggcttcg cgatctacct caaagggcga gaaattcgta gcaagaatag
     1921 cggcggacaa acgattgctg ctgatccacc taataatggc gtttgaaccc gccctattgg
     1981 attgacggtg tatttgacca acgctttatc ggtccgctaa tgaattagat ttaccccagc
     2041 gtcgaattgc cgcatcaatt aggcaccaat cgtccagagg aacaggcgtg ctgtccggcg
     2101 ttcggcggat cgcctcaggc cacaaccgtg acatcgcacc aaagtcggcc cgctgagcac
     2161 ggcggccgcg ggccgcgtcg gcagccggag ggcataaatc ttccccacgg ttaccattac
     2221 cgaccatcct ccgacgcaga gagtgcaaat caagcgggtt ccgcgcaaat aacatctaaa
     2281 gatctatcta tatagctaat tagaaactgt tgccagccaa aagcccgacg ctgaggcggt
     2341 cgcttcataa acaggccttt cggccccgac tcggttgctc ggcggccgga gcgtgcggac
     2401 caagtgccga gtgagcctct catccggagc tcgcttcgat ccgggcacca ccgtgcccat
     2461 catctggcag cggtgtccgt ccgacgcggc gccagggaat gtagactcac atatcttact
     2521 aagtaagcga tctctcgact caggatgtga cggcacgatg aagtggggac taccttggcc
     2581 cggccagcag ctggcagcag cggcggaagc tgcaggcgcc agcgccttct gcgccggtga
     2641 attcgccgat ctcagcgcat atgtgaccgc taccgagatg gcgcacgcca cgtcgaccgc
     2701 gatgatcggt cctggcatcg cctacgcctt cgcccgatca ccgtttgtgc acgccgcatc
     2761 ggtacggcat ctgtcgaagc atgccgcggg gcgggtattt ctgggcctcg gggcagggac
     2821 ctcacggatg aatcgggact ggttcggcgt cgagtccgcc cacccggcac cgcggatggc
     2881 cgagctgatc gacgtgatca gagcgttctt gcacgcggag aatggcgagc ccatccgata
     2941 tgacggcgac ttctactcca ttgacgccga catccgcgct cccgtgttcg gccggctgga
     3001 cgtgccgatc ctgatcggcg ccttcaacaa gatcatgatc cgaaccgcgg gccgcactgc
     3061 tgacggagtc ctcggacacg ggctgttcac cgaccgctgg tgggaggagg tcgtcgatcc
     3121 cgagctcgcc cgtggcgccg acggcgccgg ccgcgagcca ggcgaccttc ggcgttgggg
     3181 ctggctgatc acagcgatcg acgatgccga tccgaagcgt gccgtcgccg aggcacgcct
     3241 gcagatcgcc ttctacctga ccgtgcggac gtacgactcg ctggtcgagt tgcacggctg
     3301 gcaagacgaa gtcgccgcga tccgcgccga tttccggaac gggcgcccga aggccatggc
     3361 cgaccacgtc accgacgaca tgttgtgggc tgtggcgatc tgcggcgacg ccgcgcaggc
     3421 ccgcgacatg ctcgcgcacc gcaagcggct acccgacacg gcgttcttcg gcgcgccgag
     3481 ctttctggtc ggccgccgcc gacgtgcgga gtacgacgcg gccgctgttc gactcgcgtc
     3541 cgagctgcac tgacgcgcaa tgtcatcggc cgatccggcg tcacacatgg tttactgagt
     3601 agtacaactg caagctgtag gaggccaccg tgtctcgacc cgaatcgctc gctccaatga
     3661 tctcacccga ggcggcgtct ggcgccgtgc gatcccccaa gacagcggag ttggtcgcgc
     3721 ggacattgcg ccggatggtg gtcgaaggcc aactcaagga tggcgacttc ctgccgcacg
     3781 aggcagaact gatcacgcat ttccaggtca gcaggccgac actgcgcgaa gcggttcgtg
     3841 ttcttgagtc cgagcgactc gtcgaggtcc gccgtggctc tcgcacgggt gccaaggtgc
     3901 gggtgccggg tgccgagatc gttgcgcgac ctgcggggct cttgctcgcc ctgtccggtg
     3961 ccacgctggc agacgtgatg accgcacgga tcgcgatcga acccgcggcg gccaaactgc
     4021 tcgccgagga cggcaccgcc
//
LOCUS       DASYLX010000284         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_450531,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000284 DASYLX010000000
VERSION     DASYLX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450531"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VGP27_06900"
     CDS             <1..460
                     /locus_tag="VGP27_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7851079.1"
                     /translation="GRRLAGPGVTVAEALAAVDFVLPALEIIDSRIVDWRISIVDTIA
                     DNASSGGVVLGAHPIRPAAADLGLLGCNLFGNGELVATGASGAALGSPVVSLAWLANT
                     LGEYGDCLEAGDVVLPGSVTAAQPVQRGDVWTAQFAELGTVTARFCTGAS"
     gene            457..1242
                     /locus_tag="VGP27_06905"
     CDS             457..1242
                     /locus_tag="VGP27_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate decarboxylase"
                     /protein_id="HEV7851080.1"
                     /translation="MNTWSVDRAATELLDAQDTPAARDPLTDEWRDLDLRTAYAIQDE
                     TLCRRVERGEHVVGVKLGLTSRAKQQRMGISSPLVAWLTDVMTLPAGAPIPGGTLIHP
                     RVEPELVFVMKERLSGPGVTAPRAMSAVGRVYGGIEIIDSRYRDFRFTLPDVVADNAS
                     SARYLTGSIGLAPERLDLALEACLVEVDGVIVDSATGAAVQGHPAEALALAANTLAER
                     GLAIEPGWIVLTGGMTDAVPVSPGTAVRVHFSHLGSLVVRGGK"
     gene            1247..1444
                     /locus_tag="VGP27_06910"
     CDS             1247..1444
                     /locus_tag="VGP27_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEV7851081.1"
                     /translation="MPFIEVTLVAGRTPEQLRALITGLTDAAEAALSASRATIRVVLR
                     EVPDTHWAAGDVTIAERSKTS"
     gene            1441..>1986
                     /locus_tag="VGP27_06915"
     CDS             1441..>1986
                     /locus_tag="VGP27_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7851082.1"
                     /translation="MTEQILHVVDGDEVESFSGAFFDTVDPWTRKPWARVALGGTEDA
                     DRAVAAARRAFDEGPWPRMGLAERGAILHRLADLIMEHRDELALADTTDMGKPLADSR
                     DNDVPRSAQNFRFFADHARLCAGEALPMDTGHHAYTRFEPAGVVAAIAPWNFPLMLET
                     WKVAPALAWGNTVVLKPAEDTP"
BASE COUNT          322 a          655 c          680 g          329 t
ORIGIN      
        1 ggggcgtcgc ctcgccggtc ccggggtcac cgtcgccgaa gcgctggctg ccgtggattt
       61 cgtgctgcct gcattggaga tcatcgacag ccgcatcgtg gactggcgaa tcagcatcgt
      121 cgacaccatt gcggacaacg cttcctcggg cggggtcgtg ctcggcgcgc acccgatccg
      181 accggctgcc gcagaccttg ggcttctggg ctgcaaccta ttcggcaacg gtgaactggt
      241 cgcaacgggc gccagcggag cggctctcgg gtcgccagtg gtctcgctgg catggctggc
      301 aaacaccctg ggcgagtacg gcgactgcct cgaggccggg gacgtcgtac tcccgggctc
      361 ggtcacggcc gcccaacctg tgcagcgtgg cgatgtctgg accgcgcagt tcgccgaact
      421 cgggaccgtc accgcacgtt tctgcacagg agcctcatga acacatggtc cgttgaccgg
      481 gcggcaaccg aactgctcga cgcgcaggac acgcctgcgg cccgagatcc cctcaccgac
      541 gagtggcggg atctcgatct ccgcacggcg tacgccattc aagacgaaac gctttgtcgc
      601 cgagtcgaac gcggcgagca cgtggtcggt gtgaaactcg gtctgacctc acgcgccaag
      661 cagcagcgga tgggcatctc gtctccactg gtggcctggc tcaccgacgt gatgacgctg
      721 ccggcggggg cgccgatacc cggcgggaca ctgatccatc ctcgtgtgga accagaactt
      781 gtcttcgtca tgaaggagag gttgtccggt ccgggtgtca ctgcgccacg ggctatgtcg
      841 gccgtgggcc gcgtctatgg aggtatcgag atcatcgact cccggtatcg cgacttccgc
      901 ttcacgctgc ctgacgtggt ggccgacaat gcgtcctcgg cgcggtatct gaccgggtcg
      961 atcgggctgg cgccggagcg actggatctt gcactggagg cttgcctggt cgaagtcgac
     1021 ggcgtcatcg tcgacagcgc caccggcgcc gcggtgcagg gccacccagc cgaagcgctg
     1081 gcgctggccg ccaacaccct tgccgaaagg ggactggcga tcgagcccgg ctggatcgtt
     1141 ctcaccggcg gtatgaccga cgccgtcccg gtgagcccgg gtaccgcggt gcgggtgcac
     1201 ttctcgcacc tgggttcact ggtcgtacga ggagggaagt aacgcaatgc cgtttatcga
     1261 agtgacgctg gttgcgggcc gcacaccgga gcaactgcgc gcgttgatca ctggtttgac
     1321 ggacgcggcc gaggctgcgc tgagtgcctc gcgggcgacc atcagggtgg tgttgcgcga
     1381 ggtgcccgat acccactggg ccgcagggga cgtgacgatc gcggaacgtt ccaagacttc
     1441 atgaccgaac agatactgca cgtcgtcgac ggcgacgagg tcgaatcgtt cagcggagcc
     1501 ttcttcgaca ccgtcgatcc atggacccgc aagccgtggg cccgggtggc actgggcggc
     1561 accgaggacg ccgaccgtgc ggtcgcggcg gcgcgccggg cgttcgacga aggtccttgg
     1621 ccgcgaatgg gtttggcgga acgcggcgcg atcctgcacc ggctggcgga cctcatcatg
     1681 gagcaccgcg atgaactcgc gctcgccgac accaccgaca tgggcaagcc gctcgccgac
     1741 agccgcgaca acgacgtgcc ccggtccgcg cagaacttcc ggttcttcgc cgaccacgcg
     1801 cgactgtgcg caggcgaagc gttgccgatg gacaccggcc accacgcgta cacgcgtttc
     1861 gaaccggcgg gcgtggtcgc cgcgatcgcg ccgtggaact tcccgttgat gctcgagacc
     1921 tggaaggtcg caccagcatt ggcctggggg aacactgtcg tcctcaagcc tgccgaggac
     1981 accccc
//
LOCUS       DASYLX010000285         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1218085,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000285 DASYLX010000000
VERSION     DASYLX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218085"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGP27_06920"
     CDS             complement(<1..215)
                     /locus_tag="VGP27_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEV7851083.1"
                     /translation="MHEMAITQSVVDAVCEHAAGRRVHSVKLEVGELCAVVPDSMQFC
                     FELATQGTVADGALLDLDLRPGAARCRT"
     gene            472..1512
                     /gene="recA"
                     /locus_tag="VGP27_06925"
     CDS             472..1512
                     /gene="recA"
                     /locus_tag="VGP27_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV7851084.1"
                     /translation="MTQGPDREKALELALAQIEKSHGKGSVMRLGDEIRQPISVIPTG
                     SIALDVALGIGGLPRGRVVEIYGPESSGKTTVALHAVANAQAAGGIAAFIDAEHALDP
                     DYASKLGVDTDSLLVSQPDTGEQALEIADMLIRSGALDILVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLMSQALRKITGALSNSGTTAIFINQLREKIGVMFGSPETTTGGKALKF
                     YASVRLDVRRIETLKDGTDAVGNRTRCKVVKNKMAPPFKQAEFDILYGKGISKEGSLI
                     DMGVEHGFIRKSGSWFTYEGEQLGQGKENARTFLLENVDIANEIEKKIKEKLGIGAVV
                     TDDEVLPAPVDF"
     gene            1478..2038
                     /gene="recX"
                     /locus_tag="VGP27_06930"
     CDS             1478..2038
                     /gene="recX"
                     /locus_tag="VGP27_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV7851085.1"
                     /translation="MTKSCRPPLTSKSAELSREEQARDPRAREEQARSLCLRLLTARA
                     RTRAELAGQLAKRGYPDDVSNRVLDRMADVGLIDDADFAEQWVRSRRENAGKGKRALA
                     AELRTKGVDNELITAALANIDARAERERAEQLVSDKLRRERLTDDDAKLARRLVGMLA
                     RRGYTQTMALDVVKVALANERERRKV"
     gene            complement(2035..2871)
                     /locus_tag="VGP27_06935"
     CDS             complement(2035..2871)
                     /locus_tag="VGP27_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851086.1"
                     /translation="MAVWFITGASRGFGLEITREALSRGDSVVATARNPQAVRDAIAD
                     GGDRLHAVGLDVGVPEQVSAAVDAAIERFGRIDVLVNNAGRGLVGAVEETSDAEARAI
                     FDINVFGLLAVTRAVLPHMRAARSGLVVNLSSVGGFVAWPGWGVYAATKFAVEALSEA
                     MTHELAPLGIKSVAIEPGPFRTNFLDGSSLSVVQTRIDEYSATAGTARDWATDSNYGQ
                     EGDPVKAAKIIVDLADCDELPERIQLGANAIEDIAAKLARTATDQEQWRSISLSADFT
                     AS"
     gene            3007..3834
                     /locus_tag="VGP27_06940"
     CDS             3007..3834
                     /locus_tag="VGP27_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7851087.1"
                     /translation="MLLSAKIAPTFDYPVLEQFWRAADELGFHSVANYDHFYGLIDNT
                     VPTFEGWTSLAAMAAVVSRARVSCLVTGVTYRNPAVLAKMAVTVDHISGGRLDFGLGA
                     GWHEAEHRGYGLEFPSAGTRVAMLDEALTVIRRLWTEESVSFEGRFYTLRDALCEPKP
                     LQRPYPPIVIGGEKPKMLRVIARHADEWNVPSHGDVAEWARTSANLDEACEEVGREPS
                     EIRRSVQLFIQPGEEGHLEAQLGTLPELEACGCQHAVLSFYQPPTAQQLEKCAARIQ"
     gene            complement(3828..4742)
                     /locus_tag="VGP27_06945"
     CDS             complement(3828..4742)
                     /locus_tag="VGP27_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7851088.1"
                     /translation="MTGPSVLFDAPGRRARVRNWVITAITVVLLLLMAWVVYSKLNSK
                     GQLTAAKWEPFLTANLWKTYILPGVQGTLAAAAVSIVLALLLGFVLGIGRMSSHPAIR
                     RPCAVFVEFFRAVPVLVMMLFAFGLFALYDVFPGKQLALAGVIAGLTFYNGAVIAEIV
                     RAGVNALPRGQSEASFALGLTWGQTMRTILLPQAITSMLPVLISQMVVVLKDTAIGYQ
                     ITFLETVRQGTNIGAAYSNYIPALIVVAALMIAVNFALSWFATWLEGRMRRSRRAPTP
                     LDPKAIEQEGAPGAQVKLAALGDQSHTY"
     gene            complement(4739..>5129)
                     /locus_tag="VGP27_06950"
     CDS             complement(4739..>5129)
                     /locus_tag="VGP27_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7851089.1"
                     /translation="LAVLGLTVYTASFVCETVRSGVNTIPLGQAEAARSLGLSFGQNL
                     RIILLPQAFRAVIIPLGSVLIALTKNTTIASAIGVAEAALLMKEMIENTAALLTVGAI
                     FALGFVVLTLPLGLFFGWLGKRFAVAR"
BASE COUNT          954 a         1657 c         1726 g          792 t
ORIGIN      
        1 gtgcggcagc gggcggcgcc gggtcggagg tcgaggtcga ggagggcgcc gtcggccacc
       61 gtgccctggg tggccagctc gaagcagaac tgcatggagt cgggaaccac ggcgcacagc
      121 tcaccgacct cgagcttgac gctgtgtacg cgacgacccg cggcatgctc acacaccgcg
      181 tcgacaacgc tctgcgtaat cgccatttcg tgcatgtttg tccgctctgc cgagccggtg
      241 accccaacca taggccgatt ttctctgtga ccgcgggtga tcggcgtgtc agcttgaatc
      301 gaacacgtgt tcgtctactg tgggcagtgt tcgacggcta gatgctggga cggggcgagc
      361 gaagcgacgg gaaataaggc gtgagcgcga cttgtcggtg gcggcctcta gcgtcacggc
      421 caactgatcg acaaccgatc accgaacaca cgaggacggg agaccacccc catgacgcag
      481 ggacctgatc gcgagaaagc cctcgagctt gccctcgccc agatcgagaa gagtcacggc
      541 aaaggctcgg tgatgcggct cggcgacgag atccgccagc cgatctctgt catccccacc
      601 gggtccatcg ccctggacgt ggcgctcgga atcggcgggt tgccgcgcgg acgtgttgtc
      661 gagatctacg gtccggaatc ctcgggtaag accaccgttg cgctgcacgc ggtggccaac
      721 gcgcaggcgg cgggcggcat cgcggcgttc atcgacgccg agcacgcgct ggacccggac
      781 tatgccagca agctcggcgt ggacaccgac tcgctgctgg tgtcccagcc cgacaccggt
      841 gaacaggcgc tggagatcgc cgacatgctg atccgctccg gcgcgctgga catcctggtc
      901 atcgactcgg tggcggcgct ggtgccgcgc gccgagatcg agggcgagat gggcgacagc
      961 cacgtcggcc tgcaggcccg actgatgagc caggcgctac gtaagatcac tggcgcgctg
     1021 agcaattcgg gcaccaccgc gatcttcatc aaccagctcc gggagaagat cggcgtgatg
     1081 ttcgggtcgc ccgagacgac aacgggcgga aaggcgttga agttctacgc ttcggttcgc
     1141 ctggacgtca ggcgaattga gaccctgaag gacggcaccg acgcggtcgg caaccgtacc
     1201 cgctgcaagg tcgtcaagaa caagatggcg ccgccgttca agcaggccga gttcgacatc
     1261 ctctacggca agggcatcag caaggaaggc tcgctcatcg acatgggcgt cgagcacggt
     1321 ttcatccgca agtccggttc ctggttcacc tacgagggtg agcagctggg ccagggcaag
     1381 gagaacgccc gtaccttcct gctcgagaac gtcgatatcg ccaacgagat cgagaagaag
     1441 atcaaggaaa agctgggcat cggcgccgtg gtgaccgatg acgaagtcct gccggccccc
     1501 gttgacttct aagtcggctg agctctcccg cgaggagcag gcgcgggatc cccgcgcacg
     1561 cgaggagcag gcaagaagcc tgtgcctgcg cctgctcacc gcgcgggcgc gcacccgcgc
     1621 agagctggcc ggccagctgg ccaagcgcgg ctatcccgac gatgtcagca accgggtgct
     1681 cgaccggatg gccgacgtcg gtctcatcga cgacgccgac ttcgccgagc agtgggtgcg
     1741 gtcgcgccgg gagaacgccg ggaaaggtaa gcgcgcgttg gccgcggagc ttcgcaccaa
     1801 aggtgtcgac aacgagctca tcaccgccgc gctggccaat atcgacgccc gagccgagcg
     1861 cgagcgcgcc gagcagctcg tcagcgacaa gctgcgccgc gaacggctca ccgacgacga
     1921 cgccaagctg gcccggcggc tggtcggcat gctggcccga cgcggctata cccagaccat
     1981 ggcgttggac gtcgtcaagg tggcgctggc caacgaacgc gaacgcagaa aggtctagct
     2041 cgccgtgaag tcggccgaca ggctgatcga ccgccactgc tcctggtctg tggctgtgcg
     2101 cgcaagcttg gcagcgatat cctcaatcgc attggcgcca agctggattc gctcgggcag
     2161 ctcgtcgcag tcggcgaggt cgacgatgat cttggcggcc ttcaccgggt cgccctcctg
     2221 cccgtagttc gagtcggtgg cccagtcgcg ggccgtaccc gccgtcgccg agtattcgtc
     2281 gatccgtgtc tgcaccaccg aaaggctgct gccgtcaagg aagtttgtgc ggaacggtcc
     2341 cggctcgatg gccaccgact tgatgcccag cggcgccaac tcgtgggtca tggcctccga
     2401 cagcgcctcg accgcgaact ttgtggcggc gtagacgccc cagccgggcc atgccacgaa
     2461 accgccgacc gaagaaaggt tgaccaccag gccggaccgc gccgcccgca tgtgcggcag
     2521 gaccgctcgg gtgaccgcga gcagcccgaa cacattgatg tcgaagattg cgcgagcctc
     2581 ggcatcggag gtttcctcga ccgcgcccac taatccgcgg cccgcgttgt tgaccagtac
     2641 atcgatgcga ccgaaccgct cgatcgcggc gtccaccgcg gcgctcacct gctcgggtac
     2701 ccccacgtcg agtccgacgg cgtgcaggcg atcgccaccg tcggcaatcg cgtcgcgcac
     2761 cgcctgcggg ttgcgggcgg tcgccaccac gctgtcgccg cgggacagcg cctccctggt
     2821 gatctcgagt ccgaagccgc gggatgcccc ggtgatgaac cacactgcca tgttgaatcc
     2881 tttccctgct cagttgtcta acacaacggg gttcgcaggg catgcgactc cggagaccca
     2941 ggtacggccg gccggacgga atgcgtgcga tactgccgga gagcgtcggc cggtaggttt
     3001 gcggccatgc tgctctccgc caaaatcgct cccaccttcg actaccccgt gctggagcaa
     3061 ttctggcgcg cggccgacga actgggcttc cattccgtcg ccaactacga ccatttctac
     3121 gggctgatcg acaacaccgt gccgactttc gagggctgga cgtcgcttgc cgccatggcc
     3181 gcggtcgtgt cgcgggcgcg ggtgagttgc ttggtgacgg gtgtgacgta ccgcaacccg
     3241 gcggtactgg cgaagatggc ggtgacggtc gaccacatca gcggcggacg tctcgacttc
     3301 gggctggggg cgggctggca tgaagccgag caccgcggct acggactcga gttccccagc
     3361 gcgggaacgc gagtggccat gctggatgaa gcgctgaccg tcatcaggag actgtggaca
     3421 gaggagtcgg tcagcttcga aggccgcttc tacacgctgc gcgacgcgtt gtgtgaacca
     3481 aaacccctgc agcgaccgta tccgccgatc gtcatcggag gagagaaacc caagatgctg
     3541 cgggtcatcg cacgccatgc cgacgagtgg aacgtgccca gccacggcga tgtggcggag
     3601 tgggcgagaa ccagcgccaa tctcgacgag gcgtgcgagg aggtcggtcg ggagccgtcg
     3661 gagatccggc gctcggtgca gttgttcatc cagccgggcg aggaaggcca cctggaggcg
     3721 caactgggca cgcttcccga gttggaggct tgcggctgcc agcatgcggt gctgtcgttc
     3781 taccagccgc cgacggcaca gcagctggag aagtgcgctg cgcgcattca gtaggtatgc
     3841 gactgatcgc ccagcgcggc gagtttcacc tgggcgccag gtgcgccctc ctgctcgatc
     3901 gccttgggat ccaacggggt cggcgcgcga cgcgaccgcc gcatgcgtcc ttccagccac
     3961 gtggcgaacc aggacagcgc gaagttcacg gcgatcatca acgcggcgac cacgatcagc
     4021 gcggggatgt aattgctgta cgccgcaccg atgttcgtgc cctgcctgac cgtttcgagg
     4081 aacgtgatct ggtaaccgat cgcggtgtcc ttgagcacca ccaccatctg ggagatgagc
     4141 accggcaaca tcgacgtgat ggcctggggg aggaggatgg tccgcatggt ctggccccat
     4201 gtcagaccca gtgcgaagga cgcctccgac tggccccgcg gtagggcgtt cacgcccgcg
     4261 cgcacgattt cggcgatgac ggcgccgttg tagaacgtca ggcccgcgat cacgccggcg
     4321 agcgcgagct gcttgccggg gaagacgtcg tagagcgcga acagtccgaa tgcgaagagc
     4381 atcatcacca gcaccggcac cgcgcggaag aattcgacga aaacggcgca cggccggcga
     4441 atcgcggggt gggaggacat ccggccgatg cctaacacga agccgagcag cagcgccagc
     4501 acgatggaca ccgcggcggc cgcgagggtg ccctggacgc cgggcaggat gtacgtcttc
     4561 cacaggttgg cggtcaggaa cggttcccat ttcgccgcgg tgagctgacc cttcgagttc
     4621 agcttcgagt acaccaccca ggccatcagg agcaggagca ccacggtgat ggccgtgatc
     4681 acccagttgc ggaccctggc cctgcgcccg ggcgcatcga agagaaccga cggcccggtc
     4741 accttgccac cgcaaaacgc ttgccgagcc agccgaagaa gaggccaagc ggcaaggtca
     4801 gcacaacgaa cccgagagcg aagatggcgc cgacggtcag aagtgcggcg gtgttctcga
     4861 tcatctcctt catgagcaac gcggcctcgg caacgccgat cgccgaggcg atcgtggtgt
     4921 tcttcgtcag cgcgatcagc accgagccga gcgggatgat tacggcgcga aaagcctgcg
     4981 gcagcagaat tattcggagg ttctggccga atgacaatcc cagcgaccgc gccgcctcgg
     5041 cctggcccaa cgggatggtg ttgaccccgg atcgtaccgt ctcgcagacg aatgacgctg
     5101 tgtagaccgt caacccgagt acggcgagc
//
LOCUS       DASYLX010000286         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2097094,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000286 DASYLX010000000
VERSION     DASYLX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097094"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VGP27_06955"
     CDS             complement(<1..737)
                     /locus_tag="VGP27_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7851090.1"
                     /translation="MKRFACAFAAICAIAGPAPVAAAQSLPAPAHIVIVVEENKPDSA
                     VVGSKSAPYLNALANAGANMAQSFAETHPSEPNYLALFSGNTFGLTSDACPVNAGAAP
                     NVASELLAAGYTFGGFSEDLPAVGSTACSAGKYARKHAPWANFTNVPAANSVPFSAFN
                     NRTDYASLPTVSFVIPNLDNDMHDGTVSQADTWLYNNLSRYANWAVANNSLLIITFDE
                     DDGGSKNQIPTIIYGAHVRPGTYSEPI"
     gene            complement(893..>1580)
                     /locus_tag="VGP27_06960"
     CDS             complement(893..>1580)
                     /locus_tag="VGP27_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEV7851091.1"
                     /translation="LLGRARKVVVTKDETTIVEGAGDSEAIAGRVAQIRAEIENSDSD
                     YDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNAKAAVEEGIVAGGGV
                     ALLQSSPALDELKLTGDEATGANIVRVALSAPLKQIAFNGGLEPGVVAEKVTNSPAGV
                     GLNAATGEYEDLLKAGVADPVKVTRSALQNAASIAALFLTTEAVVADKPEKAAAPAGD
                     PTGGMGGMDF"
BASE COUNT          227 a          527 c          548 g          278 t
ORIGIN      
        1 ctgatcggct cgctgtaggt gccgggtcgg acatgggcgc cgtagatgat cgtggggatc
       61 tggttcttcg agccgccgtc gtcctcgtcg aacgtgatga tcagcaggct gttgttggcc
      121 accgcccagt tcgcgtagcg cgacaggttg ttgtagagcc aggtgtcggc ctgcgagacg
      181 gtgccgtcgt gcatgtcgtt gtccaggttc gggatcacga acgacaccgt cggcaggctc
      241 gcgtagtcgg tgcggttgtt gaacgccgag aacggaaccg agttggccgc aggcacattc
      301 gtgaagttgg cccatggcgc atgcttgcgg gcgtacttgc ccgcactgca ggcggtggac
      361 ccgaccgccg gaaggtcctc cgagaagccg ccgaacgtgt agcccgccgc caacagctcg
      421 gaggcgacat tcggcgccgc gccggcattg accgggcacg cgtccgacgt cagcccgaag
      481 gtgttgccgg agaacagcgc cagatagttc ggctcgctcg gatgcgtctc ggcgaacgac
      541 tgtgccatgt tcgcgcctgc gttggcgagc gcgttgaggt acggcgccga cttgctgccg
      601 acgactgcgc tgtcgggctt gttctcctcg acgacgatca cgatgtgcgc cggcgccgga
      661 agcgattgcg cggcggcgac gggcgccggt ccggcgatcg cgcagatagc cgcgaatgca
      721 cacgcgaagc gcttcatgcg ccgagtctag gagcccgatc gaagatgctc gaggaggcac
      781 gcgcacgacg gcgtcgagga caactcgagt ttcgggcatc cgcgggccgc cgcggcaacg
      841 aaaaagcccg gctccttgtg ggagccgggc cttctcgtcg accaggactg cgttagaagt
      901 ccatgccgcc catgccaccg gtcgggtcgc ctgcgggtgc ggcggccttc tccggcttgt
      961 ctgcgacgac cgcttcggtc gtcaggaaca gtgccgcgat ggacgcagcg ttctgcagcg
     1021 ccgaacgcgt caccttgacc gggtcggcca cgcccgcctt gagcaggtcc tcgtactcgc
     1081 cggtcgcggc gttgaggccg acccctgcgg gcgagttggt gaccttctcg gcgaccacac
     1141 cgggctcgag cccaccgttg aaggcgatct gcttcagcgg ggccgacagc gccacgcgga
     1201 cgatgttggc accggtggcc tcgtcgcccg tcagcttcag ctcgtccagc gcaggtgagg
     1261 actgcagcag ggccacgcca ccgccggcga cgatgccctc ctcgacggcc gccttcgcgt
     1321 tgcggacggc gtcctcgatg cggtgcttgc gctccttgag ctccacctcg gtggcggcgc
     1381 ctgccttgat caccgcaaca ccgcctgcca gcttggccag gcgctcctgc agcttctcac
     1441 ggtcgtagtc ggagtcgctg ttctcgatct cggcgcggat ctgcgcaacg cggcctgcga
     1501 tggcctcgct gtcaccggcg ccctcgacga tggtcgtctc gtccttggtg acgacgacct
     1561 tgcgggcgcg gcccagcagc
//
LOCUS       DASYLX010000287        13452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1987033,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000287 DASYLX010000000
VERSION     DASYLX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987033"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VGP27_06965"
     CDS             <1..856
                     /locus_tag="VGP27_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7851092.1"
                     /translation="GGSSVTDEYRQRSFAVNVGGTETLVMAGQEAGVKRFVYTASNSV
                     VMGGKRISGGDETLPYTERFNDLYTETKVVAERFVLSQNGVRGMSTCSIRPSGIWGRG
                     DQTMFRKLFESVLAGHVKVLVGGKHAKLDNSYVHNLIHGFILAAEHLVPGGTAPGQAY
                     FINDGEPINMFEFSRPVVEACGQRWPRLRISGAFVRWVMTIWQWLHFRVGLPKPPFEP
                     LAVERLYLDNYFSIAKAKRDLGYRPLYTTDEAMRDCLPYYLGLFEQMKAQAQEAPVKV
                     VPAGRPTA"
     gene            complement(872..1150)
                     /locus_tag="VGP27_06970"
     CDS             complement(872..1150)
                     /locus_tag="VGP27_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dsRBD fold-containing protein"
                     /protein_id="HEV7851093.1"
                     /translation="MYDKSLDDVWRLDIEFDEDETHTHATIRAHLGDGEVLTAIGDAF
                     RNPRDVSQPMIGEEIAAARALISLGTDLLHRAGARIEAVTRHPVHLVG"
     gene            complement(1230..1688)
                     /locus_tag="VGP27_06975"
     CDS             complement(1230..1688)
                     /locus_tag="VGP27_06975"
                     /inference="COORDINATES: protein motif:HMM:NF039955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HEV7851094.1"
                     /translation="MVQIASENASALRLALCSRSTLPGAIDGAWWPQTSDLRTELPDL
                     VAVLGMSIGPVRRVVYDPSVWPNAPSRIIRGTVLTRVDPYTMVASDTIYLMGTHARSA
                     VLFVVPPAIPVAAVRCVLRAVTDATGSISVPVLRHLVDRFAAETAPSTTF"
     gene            1911..2744
                     /locus_tag="VGP27_06980"
                     /pseudo
     CDS             1911..2744
                     /locus_tag="VGP27_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            2771..4315
                     /locus_tag="VGP27_06985"
     CDS             2771..4315
                     /locus_tag="VGP27_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV7851095.1"
                     /translation="MPETKPNILVIWGDDIGISNLSCYSRGMMGYFTPNIDRIAEEGL
                     LFTDSYGEQSCTAGRSSFITGQSVYRTGMSKVGMPGMDIGLQKEDPTIAELLKPLGYA
                     TGQFGKNHLGDLNKYLPTAHGFDEFFGNLYHLNAEEEPENPDYPTEEEAPILRKALLP
                     RGVIHSWATDEDSGEVDERYGPVGKQRIEDTGPLTKKRMETIDDETTGACIDFIRRQH
                     QDGTPFFVWMNMTHMHFRTHTKPESLGQAGRWQSPYHDTMIDHDRNVGQLLDLIDELG
                     IAEDTVVVYSTDNGPHANSWPDGATTPFRSEKATNWEGAFRIPEMIRWTGKIKPRRVS
                     NEIVQHHDWLPTFLAAAGEPDIVEKLKKGHKAGDMTYKVHIDGFNLVPYLTGEVEKSP
                     RRGMIYFSDDCDVLGIRAENWKVVFQEQRCRGTLQIWFEPFTPLRAPKLFNLRTDPFE
                     RADITSNTYWDWLIDRIYLVLYGSAIATQFLETFKEFPPRQNPATFTINHAVEELNKF
                     LASRGG"
     gene            4318..5247
                     /locus_tag="VGP27_06990"
     CDS             4318..5247
                     /locus_tag="VGP27_06990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7851096.1"
                     /translation="MGELTSWNDGATKSAIVDFVARVTEDGGPDYVVPEGRVAVFDND
                     GTLWCEKPMYIQLDFIVRRLSEKARADPSLAGQQPYLAAGTGDLNWFGGAITKHYQGD
                     DADLKVLAGAVMSLHESMTVDEHAALVAAFYAEAKHPTLRRPYQDCTYAPMVELLRYL
                     EATGFTCYIVSGGGRDFMRPITSAIYGVPPERVVGSSQGLKFDGAEGHGGLLIQPALD
                     VFDDGPEKPVRIWNRVGRRPILAAGNSNGDDEMLMYSGRPGAASLRLVVLHDDGEREF
                     DYTAGAERALEHAKQFGWTVISMKNDWATVFSD"
     gene            5324..6193
                     /locus_tag="VGP27_06995"
     CDS             5324..6193
                     /locus_tag="VGP27_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV7851097.1"
                     /translation="MARIPAQTAVVGSDHHYPEEGPARPVSVDEFWIQARQVTNAEYA
                     EFVDHTGYVTVAERPLDPADYPDAPKENLQPGSMVFHRTSGPVDLRHISQWWTWTSGA
                     SWRHPVGPHSSIDKRMDHPVVHVAYEDAETYSQWAGLALPTEAEWEIAARGGLEAAIY
                     TWGDTPEQENQRLANYWHGDFPWRPDPGYGRTAPVGSFAPNGYGLFDTAGNVWEWTTD
                     WYTDTREGAPCCEAGSYDPRQPQFRVPRRVIKGGSFLCADAYCLRYRPAARRPQMVDT
                     GMSHIGFRCVMRG"
     gene            6216..7559
                     /locus_tag="VGP27_07000"
     CDS             6216..7559
                     /locus_tag="VGP27_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7851098.1"
                     /translation="MSVKDGGVHYARNGSVRLAYRVLGEGDTTVVWNPGWFSHVDEWV
                     DPAMPFVAFVEALTRGVRLVLWDKRGTGLSDPVTDVPALDERMDDLHAVMDAVGVETA
                     AFFGMSEGGPLSILFAATYPERVQSLALYGTMMRFSPELPDHPWGWTAEQSQAMAEEI
                     ESSWGEGALVDMFFGAIADIPGFREFYGRAQRSGASPTMTLMLLQALVGCDVGGVVGS
                     VHTPTLVMSRPGDVVAPIDGARALAAAMPNARFQQLSPGPHSLLDDELAAVIVDFARG
                     RPAEDPGERILSTVLFTDIVSSTEQLVAQGDTQWRHQLDAHDKLVDWLLEKYGGRRVK
                     HTGDGMFALFDGPTKAARCGLDMVPALAARGIRIRVGVHTGECERRGEEWSGVAVHVG
                     ARIGAMAGTDHVLASRTVRDLSAGSGLRFDSLGPQQLKGLGEEIEVFRVTGPARV"
     gene            complement(7563..8009)
                     /locus_tag="VGP27_07005"
     CDS             complement(7563..8009)
                     /locus_tag="VGP27_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851099.1"
                     /translation="MSESISVAATPDELYALVSDVTRMGEWSPVCRACWWDEGGGPTV
                     GSWFTGRNESAERTWETRSQVVAAEPGRRFAWRVNHGWVHWEYTFEPDGNGTRLTEKW
                     EFLPAGIAGFRERYGEDADREIEKRRDAARSGIPETLAAIKKAAEG"
     gene            8087..8857
                     /locus_tag="VGP27_07010"
     CDS             8087..8857
                     /locus_tag="VGP27_07010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851100.1"
                     /translation="MSDSSERIAVVVGAASGIGWATARALAADGCRVTLADRNGDGAR
                     TRASELGEPHTAAEVDVTDEASVQRLFEQIGALDVVVNCAGFGSLGLITELAVDEFRS
                     VVDVCLNGGFIVAKYAGQKLREGGSLVTISSLNGRQAAVGMSAYCSAKAGVSMLTQVA
                     ALEMGPRGIRVNAISPGFVDTPLTESSHLVPGLVEDYIENTALGRAGKPEEIADAVVF
                     LCSPKSSWLTGEVLDLNGGAHLKRYPDVLCHVMKLAET"
     gene            8860..9720
                     /locus_tag="VGP27_07015"
     CDS             8860..9720
                     /locus_tag="VGP27_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851101.1"
                     /translation="MSRSGEEQTNNGFTGKQAIVTGAGSGIGAALCRALVAAGADVVC
                     TDVDGDAAERTASSLGTRARPDRLDVTDAAAVQATVNDVVNRTGRLDLMFNNAGIVWG
                     GDSELLTLDQWNAIIDVNIRGVVHGVAAAYPQMVRQGRGHIVNTASMAGLTAAGQITS
                     YVMTKHAIVGLSLALRSEAAAHGIGVLAVCPSAIETPLLDKGAIGGFDGRNYFLQGQG
                     AKSAYPADRLARDVLKAVERNKALLVKPRSAHAQWLFARLAPGLMQRASIRFIASQRA
                     HQTAAKSGSR"
     gene            9773..10996
                     /locus_tag="VGP27_07020"
     CDS             9773..10996
                     /locus_tag="VGP27_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7851102.1"
                     /translation="METEFTLTQRRALAVATVIAILFGAYFLRTYFILIVVAAVVAYL
                     FSPLYDRLNKRLGTGLSATVTLLAAFASVIVPVGLIGLIAVVQISEMVKSVAEWVERT
                     DLSTLGDKTLKLVNELLSRVPFADITVTPEMIRKAMTTVGQNGGQWLLHTLQGAAGGL
                     IGGITAAILFLYVFVSLLTNKDAVQRLIRQLNPLGEEVTDLYLAKMGAMVNGTVRGQF
                     IIAVCQGVAGAISIYIGGFHDGFFIFAIILSALSVIPLGSGVITIPFGIGMMLFGNVF
                     GGIFVVLFHLIVVTNIDNFLRPILVPREARLDSALMLLSVFAGIGMFGAFGIVIGPVL
                     MIVIVTTISVYLAVYKGVPLEQHDDDDTEKSKRKKRSLRRLTRRARRAEPEAPDPDDA
                     KSQPDAKDAAKPDAP"
     gene            complement(10977..11804)
                     /locus_tag="VGP27_07025"
     CDS             complement(10977..11804)
                     /locus_tag="VGP27_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7851103.1"
                     /translation="MTAQDPNVAGLAAPEADLTGWTAAPFTAAGYTHDVYRKGEGPGV
                     VLIPEMPGLTPNVLALGNHLVDNGFTVASPSLYGTPGAAALRPGAVPVMLRGCVAKEF
                     AAFATNADRPVAQYLRALARDLNEKTPGKGVGVIGECWSGGFALAAAVDDSVIAPVLS
                     QPSLPIGLTAKHRRDPGLSEEELKVVERRAADEGLCALGLRFSEDRLSPSERFKTLKA
                     RLGDAFEVIEISSKKGNGHDFGKMAHSVLTLEVREQHGHPAYEARKRVVEFLTERLV"
     gene            complement(11813..12445)
                     /locus_tag="VGP27_07030"
     CDS             complement(11813..12445)
                     /locus_tag="VGP27_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HEV7851104.1"
                     /translation="MTPEPEPAPRPPKPRLLQDGRDMFWSLAPLVAACIVLAAVLGMC
                     SFAPGGPGPRPAPDYDAPAALQADAHALRMPIRVPVLPEGWHSNSGSRAGIDAGRTDP
                     SGQPARAVSSTVGYLTPTGMYMSLTQSNADEDKLVGSIDPDMVPTGTQDVDGVRWVSY
                     EGEGTAEPLWTARLNGPTGVAQIAIKGAGGTDEYRTLAAAVQNQPPLPAK"
     gene            12572..>13452
                     /gene="glpX"
                     /locus_tag="VGP27_07035"
     CDS             12572..>13452
                     /gene="glpX"
                     /locus_tag="VGP27_07035"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEV7851105.1"
                     /translation="MSPSRGEAPDRNLALELVRVTEAGAMAAGRWVGRGDKEGGDGAA
                     VDAMRELVNTVSMRGVVVIGEGEKDNAPMLYNGEEVGNGDGPDCDFAVDPIDGTTLMS
                     KGLPNAISVLAVAERGAMFDPSAVFYMNKIAVGPEAVDAIDITAPIGENIRQVAKAKH
                     LSVSDMTVCILDRPRHEQLMAEVREAGARIRLITDGDVAGAISACRPNSGTDLLAGIG
                     GTPEGIITAAAIRCMGGAIQGQLAPKDDEERQKAIDAGYDLAQILSTEDLVSGENVFF
                     CATGVTDGDLLKGVQYYG"
BASE COUNT         2487 a         4398 c         4354 g         2213 t
ORIGIN      
        1 gggcggctcg tcggtcaccg acgagtaccg ccagcgcagc ttcgcggtca acgtcggcgg
       61 caccgaaacc ctggtgatgg ccggtcagga ggccggggtg aagcggttcg tctacaccgc
      121 ttccaacagc gtggtgatgg gcggtaagcg gatctccggc ggagacgaaa cgctgcccta
      181 caccgaacga ttcaacgacc tctacaccga gaccaaggtg gtcgccgagc gatttgtatt
      241 gtcccagaac ggtgttcgcg gaatgtcgac ctgttccatc cggcccagcg gtatctgggg
      301 ccgtggcgat cagaccatgt tccgcaagtt gttcgagagc gtgctcgccg gccacgtcaa
      361 ggtgctggtc ggcggcaagc acgccaagct ggacaactcc tacgtgcaca acctcataca
      421 cggtttcatc ctggccgcag agcacctggt gcccggcggg acggctcctg ggcaggcgta
      481 cttcatcaac gacggcgagc cgatcaacat gttcgagttc tcccgcccgg tcgtcgaggc
      541 gtgcggacaa cgctggccca ggctccgcat ctcgggcgcg ttcgtgcgct gggtcatgac
      601 gatctggcaa tggctgcact tccgcgtggg gctgcccaag ccaccgttcg agccgctcgc
      661 tgtcgagcgc ctgtacctcg acaactactt ctcgatcgcc aaggcgaagc gcgatctcgg
      721 ctatcggccg ctgtacacca ccgacgaggc gatgcgcgac tgcctaccgt attacctcgg
      781 gctcttcgag cagatgaagg cgcaggcgca ggaagcgccg gtcaaggtgg tcccggcggg
      841 ccgtccgacg gcctgacgat ccggaaaaga ctcaacccac caagtggacc gggtggcgcg
      901 tcaccgcctc gatcctggcg cccgcgcggt gaagcagatc cgtgcccagt gagatcaacg
      961 ctctggcggc cgcgatctcc tcgccgatca tcggctgact gacgtccctg gggttgcgga
     1021 acgcatcacc gatcgcggtc aagacttctc cgtcaccgag atgagcgcgg atcgtcgcat
     1081 gcgtatgcgt ctcgtcctcg tcgaactcga tgtcgaggcg ccaaacgtcg tccagagact
     1141 tgtcgtacat atacgccacc ttcccgccta tagagtgctc cgctgctcgg ctgcgcatcg
     1201 ggaaatggcg cgtttgctgc aatccggccc tagaacgtgg tcgacggggc ggtctccgcg
     1261 gcgaaccggt cgaccaggtg tcgcagcacg ggcacactga ttgacccggt cgcgtcggtg
     1321 acggcacgca gcacgcaacg gacagcagcg acggggatcg cggggggcac gacgaagagc
     1381 accgcagacc gggcatgcgt gcccatcagg tagatcgtgt cactggcaac catcgtgtac
     1441 ggatcgactc tggtcagtac ggtgccgcga atgattcgcg acggcgcatt cggccacacg
     1501 ctggggtcgt agaccacccg acgaacggga ccgattgaca tccccagaac ggcgacgagg
     1561 tcaggcagtt cggtccgtag gtcggaagtc tgcggccacc aagcgccatc gatcgcgccc
     1621 ggcaatgtcg aacggctgca caacgccaac cggagcgcac tcgcattctc ggaagcgatc
     1681 tgcaccatcg ggcgctgctc cagtcaggta gtcgagcaat cgcagaccgc agccgagatg
     1741 tcggacacgc gacgaaccta cgattcattg accataaccc acaccagaat ttcgacgtct
     1801 ccgaaaattt ttcctgagag gctgctgaag cggcccaacg ctgaccggca cccgaataag
     1861 gtcaagtcgc acatcggatt cggtcggggc gtaggcaaaa ggggcgtcgc atgcctgaag
     1921 gcaggccgaa catcctggtc gtctggggcg acgacatcgg gatcagcaat ctgagttgtt
     1981 atagcgacgg tttgatggac taccgcaccc cgaacatcga ccgcattgcc gacgaaggta
     2041 tgcgattcac cgactcctat ggtgagcaaa gctgcacagc gggccgcgcg gcgttcatca
     2101 gtgggcaaag cgtctaccgc accggcatga gcaaggtcgg cgtgcccggc gctgacatcg
     2161 gatggtcggc cgacgatccc accatcgccg agctgctcaa accgctcggc tatgcgaccg
     2221 ggcagttcgg caagaaccac ttcggcgaca agaacaaaca tctgccgacg gtgcacgggt
     2281 tcgacgagtt cttcggcaac ctgtaccacc tcaacgccga ggaagagccc gagcaattcg
     2341 actacccgca cgccgaccag ttcccgaagc tccacgactt cgcgttaccg cgcggcgtgc
     2401 tgaagtgccg ggccaccgac gaggactcga ccgagccgga cgacccgaag tacggcccgg
     2461 tcggcaagca ggtcatcgag gacaccgggc cgctggacac caagcggatg gagaccatcg
     2521 acgacgacat cgccgaggcg accgtggact acatcaagcg tcagcacgcc gagggcaacc
     2581 cgttcttcgt ttggtgcaac ttcacccata tgcacgcgtt cactcacacc aagcccgaaa
     2641 gcctcgggca ggcggggctg tggcagtccg gataccacga cacgatgatc gatcacgaca
     2701 agaatgtcgg caccgttctt ggcgaccagg ccgtcgaaaa gctccataaa ttcctagcga
     2761 aggattagcc atgcctgaaa cgaagccgaa cattctcgtc atctggggcg acgacatcgg
     2821 tatctcgaat ctgagctgtt acagccgcgg aatgatgggc tacttcacgc ccaacatcga
     2881 ccggatcgcc gaggaaggac tgcttttcac cgactcctac ggcgaacaga gctgcaccgc
     2941 cggtcgttcg tcgttcatca ccggccagag cgtgtaccga accggcatga gcaaggtcgg
     3001 gatgcccggt atggacatcg ggttgcagaa agaagacccg accatcgccg agttgctcaa
     3061 accgctcggc tatgcgaccg gccagttcgg caagaaccac ctcggtgacc tgaacaagta
     3121 cctgccgaca gcgcacggct tcgacgagtt cttcggcaac ctgtaccacc tcaacgccga
     3181 ggaagagccg gagaaccccg actaccccac cgaggaagag gcgccgatac tgcgcaaggc
     3241 gttgttgccg cgcggtgtca tccattcatg ggccaccgat gaggattccg gcgaggtgga
     3301 cgagcggtac ggcccggtcg gcaaacaacg catcgaggac accggaccgc tgaccaagaa
     3361 gcggatggag accatcgacg acgaaaccac cggcgcgtgc atcgatttca tccgccgcca
     3421 gcatcaggac ggcacaccgt tcttcgtctg gatgaacatg acgcacatgc acttccggac
     3481 acataccaag ccggagagcc tcggccaggc cggccgctgg caatcgccct accacgacac
     3541 gatgatcgat cacgaccgca atgtcggcca gttgctcgat ctgatagacg aactgggaat
     3601 cgccgaggac accgtcgtcg tgtattcgac ggataacggg ccgcatgcca atagttggcc
     3661 ggacggcgcg accacaccat tccgcagtga gaaggccacc aactgggaag gcgccttccg
     3721 gattccggag atgattcggt ggaccgggaa gatcaagcca cgccgtgttt ccaacgagat
     3781 tgtccagcat catgattggt tgccgacgtt cctggccgcc gccggcgaac ccgacatcgt
     3841 cgaaaagctc aagaagggac acaaggccgg cgatatgacc tacaaggtcc atatcgacgg
     3901 cttcaacctg gtgccctacc tgaccggtga ggtggagaag agcccacggc gagggatgat
     3961 ctacttctcc gacgactgtg atgtgctggg catccgcgcc gagaactgga aggtggtgtt
     4021 ccaagagcag cgctgccgag gcaccctgca gatctggttc gaaccattca ccccgctgcg
     4081 tgccccgaag ttgttcaacc tgcggaccga cccgttcgag cgtgcggata tcacgtcgaa
     4141 cacatactgg gactggctga tcgaccgcat ttacctggtg ctctatgggt cggccatcgc
     4201 gacgcaattc ctcgaaacgt tcaaggaatt cccgccacgg cagaaccccg cgaccttcac
     4261 catcaaccat gcggttgaag aactcaacaa gttcctggca tcccggggcg gctgagagtg
     4321 ggcgaactca cttcgtggaa cgacggggcc acgaagtcgg cgatcgtcga cttcgttgct
     4381 cgggtaaccg aggacggcgg gccggattac gttgtgccgg agggtcgcgt cgcggtgttc
     4441 gacaatgacg gcacgctgtg gtgcgagaag ccgatgtaca ttcaactcga cttcatcgtc
     4501 cgcaggctca gcgagaaggc cagagccgat ccgtcgctgg ccgggcagca gccgtatctg
     4561 gccgccggca ccggcgatct gaactggttc ggcggcgcca tcaccaagca ctaccagggt
     4621 gacgacgctg acctgaaagt acttgccggc gcggtgatgt ccctgcatga gtcgatgact
     4681 gtcgacgaac acgccgccct ggtagccgcc ttctacgcgg aggccaagca cccgacgctg
     4741 aggcgcccgt accaggactg cacgtatgca ccgatggtgg agttgctgcg ctacctcgag
     4801 gcaaccggtt tcacgtgtta catcgtctcc ggtggtggcc gcgacttcat gcgtccgatc
     4861 accagtgcca tctacggggt cccgcccgaa cgggtcgtcg gcagctcgca ggggctgaag
     4921 ttcgacggcg cggaaggcca tggcggcctg ttgatccaac ccgcgctcga cgtcttcgac
     4981 gacgggccgg agaagccggt gcgcatttgg aaccgcgtcg gccgccgtcc gattcttgcc
     5041 gcgggcaact ccaacggcga cgacgagatg ttgatgtatt cgggtaggcc cggagcagcg
     5101 tcactgcgtc tggtggtgct gcacgacgac ggggagcggg agttcgatta caccgcaggc
     5161 gcagaacgcg cgctcgagca cgccaagcag ttcggctgga cagtgatcag catgaagaac
     5221 gactgggcga ccgtcttcag tgactgacaa ggatgcgccc aaaccaacgt atgggcgaga
     5281 aagtgcgagt aaaaggcgcc aaatcgtcga tctcgatgag gatatggcgc ggattccggc
     5341 gcagactgca gtggtcggat ccgaccacca ctaccccgaa gaggggccgg cacgccccgt
     5401 cagcgtcgac gagttctgga ttcaggcgcg ccaggtgacc aacgccgaat atgccgaatt
     5461 cgtcgatcac acaggctatg tcacggttgc cgaacgaccg ctcgatcccg ccgactaccc
     5521 ggacgcgccg aaagagaacc tgcagccggg gtcgatggtc ttccaccgca cctcgggtcc
     5581 cgtcgacctg cggcatatca gccagtggtg gacgtggaca tcgggcgcgt catggcgcca
     5641 ccctgtcgga ccgcattcat cgatcgacaa gcgcatggac catccggtgg tgcacgtcgc
     5701 ctatgaggac gccgaaacct attcccagtg ggcgggtctc gcgctgccga ccgaggcgga
     5761 gtgggagatc gcggcccgcg gcggcctcga ggcagcgatc tacacgtggg gcgatacacc
     5821 cgagcaggaa aaccagcggc tggccaacta ctggcacggg gacttcccgt ggcgccccga
     5881 tcccgggtac ggccgcaccg cgcccgtcgg cagctttgcg cccaatgggt acggcctgtt
     5941 cgacacggcg ggcaacgtct gggagtggac gaccgactgg tacaccgaca cccgcgaagg
     6001 cgccccgtgc tgcgaggcag gcagctacga cccgcgacag ccccagttcc gcgtgccgcg
     6061 ccgggtgatc aagggcggct ccttcctgtg cgccgacgcc tactgcctgc gctatcggcc
     6121 cgccgcccgc agaccccaga tggtcgacac cggcatgagc cacatcggct ttcgatgtgt
     6181 aatgcgcgga taaaccggct cgttgctgtg agtatgtgag tgtgaaggac ggcggagtcc
     6241 actacgcgcg caacggctcg gtccggctgg cctaccgcgt gctcggcgag ggcgacacga
     6301 ccgtcgtgtg gaatcccggg tggttcagtc acgtcgacga gtgggtcgac cccgcaatgc
     6361 cgtttgtggc gttcgtcgag gcactgaccc gcggcgtccg gctggtcctg tgggacaagc
     6421 gcgggaccgg gctgtcggat ccggtcaccg atgtaccggc cctcgacgag cggatggatg
     6481 atctgcatgc ggtgatggat gccgtcggag tcgaaaccgc cgcctttttc ggtatgtccg
     6541 aagggggccc gctgagcatc ctgttcgctg cgacgtatcc ggagcgcgtc cagtcgctgg
     6601 cgctctacgg cacgatgatg cggttcagcc ccgagttgcc ggatcacccg tggggttgga
     6661 ccgccgagca gagccaagcg atggccgaag agatcgaatc cagttggggc gaaggcgctc
     6721 tcgtggacat gttcttcggg gcgatcgcgg acataccggg cttcagagag ttctacggac
     6781 gggcgcagcg gtcgggtgcc agcccgacga tgacgctcat gctgctacaa gcgctggtcg
     6841 ggtgcgatgt gggcggtgtg gtgggctcag ttcacacccc gacccttgtc atgtccagac
     6901 ctggcgacgt ggtcgctccc attgatggag cgagggcatt ggcggcggcc atgccgaacg
     6961 cccggtttca acaactgtcg ccgggtccgc attcgttgtt ggacgacgaa ctcgcggcgg
     7021 tcattgtgga cttcgcgcgc ggtaggcccg ccgaggaccc tggcgagcgc attctgtcga
     7081 cggtgttgtt cacggacata gtcagctcca ccgaacagct cgtcgcacag ggcgacacgc
     7141 agtggcgcca tcaactcgac gcgcacgaca agctcgtgga ctggctgctg gagaagtacg
     7201 gcggccgccg cgtcaagcac acgggcgacg gcatgttcgc gctcttcgac ggtcctacca
     7261 aagcggcccg ctgcggcctc gacatggttc ctgctttggc cgccagagga attcgtatca
     7321 gggtcggcgt gcacaccggc gaatgcgagc ggcgcggcga ggagtggagc ggggtggccg
     7381 tccatgtcgg cgcgcgaata ggcgcgatgg ctggtaccga tcacgtgctc gccagccgaa
     7441 ccgtccgcga cctctcggcc ggatcgggtc tgcgcttcga cagcctgggc ccgcaacagc
     7501 tcaagggcct cggcgaggag atcgaggtgt tccgggtgac cgggcccgcc cgcgtttagc
     7561 cgtcagcctt cggccgcctt cttgatcgcg gcgagcgtct cgggaatgcc gcttctggcc
     7621 gcgtcgcggc gcttttcgat ctcccggtcg gcatcctcgc catagcgctc tcggaaaccg
     7681 gcgatgccgg caggcaggaa ctcccacttc tcggtcagcc gggtgccgtt accgtccggc
     7741 tcgaaggtgt actcccaatg cacccagccg tggttgaccc gccaggcgaa cctgcgaccg
     7801 ggttcggcgg ccaccacctg gctacgggtc tcccacgtcc gctccgcgga ctcgttgcgg
     7861 ccggtgaacc aggagcccac cgtgggcccg ccgccctcgt cccaccagca cgcccggcac
     7921 accgggctcc actcccccat cctggtcacg tcggacacca acgcgtacaa ctcgtcgggt
     7981 gtcgccgcga cggaaatcga ctcggacatc ttcagatcgg tcacggatcg agtgtgtcaa
     8041 agcgctaagt tggactgcgt tccaccgccg tcgaaaggca aactggatga gcgacagtag
     8101 tgagcggatc gccgtcgtcg tcggcgcggc gtccggcatc ggctgggcta ccgcacgcgc
     8161 cctggccgcg gacggctgcc gcgtgaccct cgccgaccgc aacggtgacg gggctcgaac
     8221 ccgggcgagt gaactcggcg aaccgcacac cgcagcggaa gtcgacgtca ccgacgaggc
     8281 gtcggtgcaa cggctcttcg agcagatcgg tgctctcgac gtcgtcgtca actgtgccgg
     8341 tttcgggagc ctggggttga tcaccgagct ggcggtcgac gagttccgct cggtggtcga
     8401 cgtctgcctc aacggcgggt tcatcgtcgc caagtacgcc ggccagaagt tgcgcgaggg
     8461 cggctcgctg gtgacgatca gctcactcaa cggacgccaa gccgccgtcg ggatgagcgc
     8521 gtactgctcg gccaaggcgg gcgtgtcgat gctcacccag gtcgccgcac tcgagatggg
     8581 accgcggggt atccgtgtca acgcgatctc ccccggcttc gtcgacactc cgctgaccga
     8641 aagctcccac ctggtaccgg gtctcgtcga ggactacatc gagaacaccg cgctcggccg
     8701 cgcaggcaag ccggaggaga tcgccgacgc cgtggtattc ctgtgctcgc cgaagtcgtc
     8761 gtggctgacc ggtgaggtgc tcgatctcaa cggcggcgcg catctcaagc gctacccgga
     8821 cgtgctgtgc cacgtcatga aactcgcgga gacgtagcga tgagccgctc gggcgaagaa
     8881 cagacaaaca acggtttcac cggtaagcaa gcgatcgtca ccggcgccgg gtccggcatc
     8941 ggcgccgcgt tgtgccgggc gcttgtcgcc gcgggcgccg acgtggtgtg cacggacgtc
     9001 gatggcgacg ccgccgagcg caccgcctcg tcgcttggaa ctcgcgcgcg tcccgatcgc
     9061 ctcgacgtca cggacgcggc ggcggtgcag gcaacggtga acgacgtggt gaaccgcacc
     9121 ggccggctgg atctgatgtt caacaatgca ggcatcgtgt ggggcggcga ctccgaactg
     9181 ctcaccctcg accagtggaa cgcgatcatc gacgtgaaca tccgcggcgt cgtgcacggc
     9241 gtcgccgcgg cgtacccgca gatggtccgg cagggccggg gccacatcgt gaacacggct
     9301 tcgatggcag ggctgaccgc ggcgggtcag atcaccagct atgtgatgac gaaacacgcc
     9361 atcgtcgggc tgtcgctcgc gctgcggtca gaggcggccg cgcacgggat cggcgtgctg
     9421 gccgtgtgtc catcggccat cgaaacaccc cttctcgaca agggcgccat cggcgggttc
     9481 gacggccgta actacttcct ccaaggccag ggagcgaagt ccgcctaccc tgccgaccgc
     9541 ctcgcacggg acgtgctgaa ggccgtcgaa cgcaacaagg cgctgctggt gaaaccgcgc
     9601 agcgcccacg cccagtggtt gttcgcgcgg ctggcgccgg ggctgatgca gcgcgcgtcg
     9661 attcgcttca tcgcgagcca gcgagcccac cagacggcag ccaagtcggg ctctcgctga
     9721 gcaaaccgga cggccggacc gcgcccgtgg ttacggtatg cgtgtgcccg cgatggagac
     9781 cgagttcacg ctgacacagc ggcgtgcgtt ggcggtcgcg acggtcatcg caatcctgtt
     9841 cggtgcctac ttcctgcgca cgtacttcat cctcatcgtg gtcgccgcag tggtcgccta
     9901 tctcttcagc cccctgtacg accggctcaa taagcggttg gggacgggac tgtcggcgac
     9961 ggtcaccctg ctggctgcct ttgcgagcgt catcgtgccg gtcggcctga tcgggctgat
    10021 cgccgtcgtc cagatcagcg agatggtcaa gagcgtcgcc gagtgggtcg agcgcaccga
    10081 cctgtccacg ctcggcgaca agacgttgaa actcgtcaac gaactgctct cgcgggtgcc
    10141 gttcgccgat atcacggtga ctcccgagat gatccgcaag gcgatgacga ccgtcggcca
    10201 aaacggtggg cagtggctgc tgcacacgct tcagggcgcc gccggaggcc tcatcggcgg
    10261 gatcaccgcg gcgatcctgt tcctctacgt gttcgtctcg ctgctgacca acaaagatgc
    10321 cgtgcaacgg ttgatccgcc agctcaaccc gctcggcgag gaggtcaccg acctctacct
    10381 cgccaagatg ggcgcgatgg tgaacggcac cgtcagaggc caattcatca ttgccgtctg
    10441 ccagggtgtc gcgggcgcga tctcgatcta catcggcggc ttccacgacg ggttcttcat
    10501 cttcgccata attctgagtg cgctgtcggt catcccgctc ggcagcggtg tcatcacgat
    10561 tcccttcggc atcggaatga tgctgttcgg caacgtgttc ggcggtatct tcgtggtcct
    10621 gttccacctc atcgtggtga ccaacatcga caacttcctc cggccgatcc tggtaccgcg
    10681 ggaggcccgc ctcgactctg cgctgatgct gctgtcggtg ttcgccggca tcggaatgtt
    10741 cggcgcgttc ggcatcgtca tcggacctgt cctgatgatc gtcatcgtca caacgatcag
    10801 cgtctatctg gcggtgtaca agggcgttcc gctcgaacag cacgatgacg acgacaccga
    10861 gaagtccaag cggaagaagc gctcattgcg gcgcttgacg cgcagggcca gacgagcaga
    10921 acccgaggcc cccgatcccg acgacgcgaa atcccagccg gacgcgaaag acgccgctaa
    10981 accagacgct ccgtgaggaa ttcgacgacg cgtttgcgcg cctcgtaggc gggatggccg
    11041 tgctgctcgc gcacctcgag cgtgagcacc gagtgcgcca tcttgccgaa gtcgtgcccg
    11101 ttgcccttct tcgagctgat ctcgatgact tcgaacgcgt cgccaagccg ggccttcaga
    11161 gtcttgaacc gctcgctggg cgacagccgg tcctcgctga aacgcagacc cagcgcacac
    11221 agcccctcat cggcggcacg ccgctcgacg actttcagtt cctcctcgga gagcccggga
    11281 tcgcgacgat gcttcgccgt caacccgatc ggcaacgagg gctggctcag caccggcgcg
    11341 atcacgctgt cgtccacggc agccgccagc gcgaacccgc cgctccagca ctcgccgatc
    11401 accccgacgc ctttaccggg cgtcttctcg ttgagatcgc gggcgagcgc ccgcagatac
    11461 tgcgccaccg gccggtccgc gttcgtcgcg aatgccgcga attcctttgc cacgcaaccg
    11521 cgcagcatca ccggcacggc accgggtcga agcgccgcag cgcccggcgt gccgtacagc
    11581 gatggagatg cgacagtaaa accgttgtcc accaagtgat tgcccaacgc cagcacgttc
    11641 ggagtcaggc ccggcatttc cgggatcagc accacgccgg gcccctcgcc cttgcggtac
    11701 acgtcgtgcg tgtagccggc ggcggtgaac ggcgcggccg tccatccggt cagatcggcc
    11761 tccggagccg ccaggccggc gacattgggg tcctgcgcgg tcacggcggc tcctatttcg
    11821 cgggcagtgg cggctggttt tggaccgccg ccgccagcgt acggtactca tcggtaccac
    11881 cggcaccctt gatcgcgatc tgcgccactc cggtcgggcc gttcaaccgc gccgtccaca
    11941 gcggttcggc ggtgccctcg ccttcgtatg acacccatcg cacgccgtcg acgtcctgcg
    12001 ttccggtcgg caccatgtcg gggtcgatag agccgaccag cttgtcctcg tcggcgttgc
    12061 tctgggtcaa cgacatatac ataccggtcg gcgtcagata gccgacggtc gaagacaccg
    12121 cgcgggcagg ctgtccagac ggatcggtgc ggcccgcatc gatgcctgcg cgactaccgg
    12181 agttggaatg ccatccctcg ggcagcacag gcacccggat gggcatgcgg agcgcgtggg
    12241 cgtcggcctg caatgccgcc ggtgcgtcgt agtcgggcgc cggtctcgga ccggggccgc
    12301 ccggcgcgaa cgagcacatc ccgagcacgg cggcgagcac gatgcatgcc gcgaccagcg
    12361 gcgcgagaga ccagaacatg tcgcggccgt cctgcagcaa ccgcggcttc gggggtctgg
    12421 gagcgggttc cggctctggg gtcacaacag ccagtatccc agctcccaca ggcggaccga
    12481 tgaatggaag aatcgctgcc atgagcaccc gaaggcgagc gcgagcgagg atcgcagcga
    12541 agcgaggacc ggaacgagcg ggagctgagg catgagccca tcccgaggag aggcccccga
    12601 ccgaaacctc gcccttgagc tcgttcgggt caccgaggcg ggcgcgatgg cagcaggccg
    12661 ctgggtcggc cgcggcgata aagagggtgg tgacggcgcc gccgtcgacg caatgcgcga
    12721 gctggtcaac acggtgtcga tgcgcggcgt cgtggtgatc ggcgagggcg agaaggacaa
    12781 cgcgccgatg ctctacaacg gcgaggaggt cggcaacggc gacgggccgg attgcgattt
    12841 cgccgtcgac ccgatcgacg gcaccacgct gatgagcaag ggcctgccca atgccatctc
    12901 cgtgctggcc gtcgcggaac gcggcgcgat gttcgatccg tcggcggtct tctacatgaa
    12961 caagatcgcg gtcggacccg aggccgttga cgccatcgac atcaccgcgc ccatcggaga
    13021 gaacatccgc caggtcgcca aagccaagca tctgtccgtg tcggacatga ccgtgtgcat
    13081 cctcgaccgg ccccggcacg agcagttgat ggccgaagtc cgagaggccg gcgccaggat
    13141 tcggctcatc accgacggcg acgttgcggg ggccatctcc gcctgcaggc cgaactccgg
    13201 cacggacctg ctcgccggta tcggcggcac accggaaggc atcatcaccg cggccgcgat
    13261 tcggtgcatg ggcggtgcca tccagggtca gctggccccg aaggacgacg aagagcgaca
    13321 gaaggccatc gacgccggat atgacctcgc ccagattctg tccaccgagg acctggtctc
    13381 cggcgagaac gtcttcttct gcgcgaccgg cgtcaccgac ggcgacctgc tcaagggtgt
    13441 ccagtattac gg
//
LOCUS       DASYLX010000288         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1767230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000288 DASYLX010000000
VERSION     DASYLX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VGP27_07040"
     CDS             complement(<1..1084)
                     /locus_tag="VGP27_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV7851106.1"
                     /translation="MVDVAVSGAQRQSLVLRFADVGVATYASLRVVGAPSQTVTWAVE
                     EPTVVAALQKLAAALPDPHGSETQRDAVERAIAAGPLASPSAESVIARDLGARLLAPR
                     AWQLLTECTASPRAALFISPSARLARVPWAALAMPGDDEFRLIEFVDVLMAAPPNIVN
                     APRTPGDWGGRQESRPLLILDPRVPGQRPDSPLGSVLGKPSPETPLARYFGELLARRP
                     VLPGVTTPVELFRRRDAGRAWLAGMLAQAPSRLLYVGHATAADGDVGHADRAALYLAD
                     DRPLTASDLMSAKLPFPTRVALLACASGGDYQFDEATGLVAAMILCGAQLVTATLWSL
                     PTAAGYRQFAPNTVAPAVDPMVEAIIA"
     gene            complement(1066..>1842)
                     /locus_tag="VGP27_07045"
     CDS             complement(1066..>1842)
                     /locus_tag="VGP27_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851107.1"
                     /translation="GLIAMSLDKLDDADERFGHAERHWREVEKPLHIHRILLQRSWID
                     ILRNRFDAATEKATEARELLDSWPRHSWLQYARLDDHLGSIRRAEALADPANAAAKLA
                     QAADLKVPAALAVDSVRHAIVDADARMRWATLVSARILAGAFAVAYEWGNTDLLSELV
                     EYHCARGAFSTEPAESGGDDWMGAATAAVPVEADDEYALVAAGSAAASGPAGLTRLGP
                     LPPLRMEPDMPPIMSRYRELAHDRYDRDITADEAAWSTWP"
BASE COUNT          291 a          648 c          626 g          277 t
ORIGIN      
        1 cggcgatgat cgcctcgacc atcggatcaa ctgccggcgc aacggtattc ggcgcgaact
       61 gccggtaacc ggccgcggtg ggtagcgacc acagtgtggc ggttaccagt tgcgcaccgc
      121 acagaatcat cgcggccacc aagcctgtcg cctcgtcgaa ctgatagtcc ccgcccgacg
      181 cgcaggccaa cagcgcgacg cgggtcggaa acgggagctt ggccgacatc aggtccgatg
      241 cggtcagtgg tcggtcgtcg gccaggtaaa gcgccgcgcg gtccgcgtgg ccgacatcgc
      301 cgtcggcggc cgtcgcgtga ccgacgtaga ggagcctgct cggcgcctgc gcgagcatgc
      361 ctgccagcca tgcgcgccca gcatccctgc gccggaacaa ctcgaccggc gttgtgacgc
      421 cgggaagtac aggccgccgt gccagtagct ctccgaaata gcgcgccagc ggagtttccg
      481 gcgacggttt gccgagaacc gagccgagcg gcgagtcagg ccgctgaccc ggtacccgcg
      541 gatcgagtat cagcagcggc cggctttcct gcctgccgcc ccagtcaccg ggtgtgcgcg
      601 gtgcgttgac gatgttcggc ggcgccgcca tcaacacgtc gacgaactcg atcaggcgaa
      661 attcgtcatc gcctggcatc gcgagggcgg cccatggcac ccgggccaat cgcgcgctgg
      721 gcgagatgaa cagtgccgca cggggcgacg ccgtgcactc cgtgagcaac tgccacgccc
      781 gcggcgccag tagccgggcg cccaggtcac gggcgatcac cgattcggcc gacggcgagg
      841 cgagcggacc agcggcgatc gcgcgctcga cggcgtcccg ctgggtctcc gagccgtgcg
      901 ggtccggcag cgccgcagcc agtttctgca gcgccgcaac cacagtgggc tcctcgaccg
      961 cccatgtgac cgtctgtgac ggcgctccga cgacgcgcag gctggcatat gtggcgacgc
     1021 cgacgtcggc gaagcgcaga accaggctct gccgctgcgc accgctcacg gccacgtcga
     1081 ccaagcggcc tcgtcggcgg tgatgtcgcg gtcgtaccga tcatgcgcca gctcgcgata
     1141 cctgctcatg atcggcggca tatcgggttc catgcgcagc ggcggcaacg ggccaaggcg
     1201 ggtcaatccg gccgggccgg aagccgccgc cgaacccgcg gccaccaggg cgtattcgtc
     1261 gtcggcctcc acaggcactg cggcagtcgc ggcgcccatc cagtcgtcgc cgccgctctc
     1321 cgccggctca gtgctgaaag cacctcgcgc acagtggtat tcgacgagtt cgctaagcag
     1381 atcggtgttg ccccattcat aggccaccgc gaacgctccg gccaagatcc gtgccgatac
     1441 cagtgtcgcc catcgcatcc gtgcgtcggc gtcgacgatg gcgtgccgca ccgaatccac
     1501 cgcgagggca gcgggcacct tcaagtcggc cgcctgcgca agtttggccg cagcgttcgc
     1561 ggggtcggcc agcgcctcgg cacgccggat gctgcccagg tggtcgtcca gccttgcgta
     1621 ttgcaaccag ctgtggcgcg gccaggagtc gaggagctcg cgtgcctcag tagccttctc
     1681 ggtcgccgcg tcgaacctgt ttcgcaggat gtcgatccag ctgcgctgca gcaggatgcg
     1741 gtggatgtgc agtggtttct ccacctcgcg ccagtgccgc tcggcgtggc cgaaacgctc
     1801 gtcggcgtca tcgagcttgt ccagcgacat ggcgatcagg cc
//
LOCUS       DASYLX010000289         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_450775,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000289 DASYLX010000000
VERSION     DASYLX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450775"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /gene="kdpA"
                     /locus_tag="VGP27_07050"
     CDS             complement(<1..1445)
                     /gene="kdpA"
                     /locus_tag="VGP27_07050"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV7851108.1"
                     /translation="MSDTVAGLLQVVLLLVMLVACYVPFGDYMARAFTSERHWRVESV
                     LYRLVRVDPSTGQRWPTYAAGVLGFSFVSVVLLYLLQRLQPFLPMSFGRGAVAPPMAF
                     NTAVSFVTNTNWQSYVPEQVLGNTVQMAGLTVQNVLSAAVGMAVAVALIRGFVRSGTD
                     RIGNFWVDLTRGAVRILLPFSFVAAILLIAMGVTMSLRSGVDVTGVDGAVHTLPLAPT
                     ASQEAIKELGTNGGGIFNANSAHPFENPNGLSNLFEIFLLLVIPVCLTRTFGTMVRNP
                     RQGTVLVSVMGVLWAALLAVAWFAETHPNGAATLAAGGALEGKETRFGIPSSILFGIS
                     TTGTSTGAVNSMHDSYTGGAGGAVLLNILLGEVAPGGVGTGLYGILVLAVIAVFLAGL
                     LVGRTPEYLGKKLGRREVTAAAVSILVMPTLVLIGAGLAIALPGTGDALNNTGAHGFS
                     EVLYAYASAANNNGSAFAGLTVTSNFFQTTLG"
     gene            complement(1445..1546)
                     /gene="kdpF"
                     /locus_tag="VGP27_07055"
     CDS             complement(1445..1546)
                     /gene="kdpF"
                     /locus_tag="VGP27_07055"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEV7851109.1"
                     /translation="MSTADVVTNVIGGIVALALLVYLMVALLRPERF"
     gene            complement(1543..1719)
                     /locus_tag="VGP27_07060"
     CDS             complement(1543..1719)
                     /locus_tag="VGP27_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851110.1"
                     /translation="MFEPLRGHCGRIHTFVGFAGRSGGQEFEELSVADVLYVLLLVGG
                     FVVLALCLRGLERL"
     gene            1913..2797
                     /locus_tag="VGP27_07065"
     CDS             1913..2797
                     /locus_tag="VGP27_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851111.1"
                     /translation="MNLPDPREARGHRLGTPLSTRGGFLAGLIAATAAPLLAWTLDAR
                     AVAVGGLVLAALAAVLIAALSTTAAAIDTAAVCWAAYDGFALNRLGELHLAAADLRAL
                     GYIVGAALSFRCLAAALRAVRAAARTSTELSIPLPAELIADLSPRPNLRPSAHSRPRR
                     PDSRLRRDRRPQNPQPAVQAPIPTAGPTDGQTGDRSWLRSQRRPRTQAPARSGALHAP
                     QFLDENLIVMSKEWASTRMGHRDEAARPRPAPHTPVTPAGLHCPARQEATRRRPAEQL
                     EDELRPCPLLGIELRGFR"
     gene            complement(2914..3069)
                     /locus_tag="VGP27_07070"
     CDS             complement(2914..3069)
                     /locus_tag="VGP27_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851112.1"
                     /translation="MPVTTTSTPVVGMRWVADNQLRGAAVPDFGGLPNPPSYEKINID
                     CHVAPSD"
     gene            3351..>3491
                     /locus_tag="VGP27_07075"
     CDS             3351..>3491
                     /locus_tag="VGP27_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851113.1"
                     /translation="MGTITTTDGTEIFYKDWGSGPPIVFSHGWPLSGDDWDTQMLFFL
                     QHG"
BASE COUNT          614 a         1258 c         1126 g          493 t
ORIGIN      
        1 ccgagcgtgg tctggaagaa gttgctggtc accgtgaggc cggcgaacgc ggaaccgttg
       61 ttgttggcgg cgctggcata ggcgtagagg acctcggaga agccgtgcgc accggtgttg
      121 ttcagcgcgt cgcccgtacc gggcagcgcg atcgccaggc ccgccccgat cagcaccagg
      181 gtcggcatga ccaggatcga caccgcggcc gcggtgacct cgcggcggcc cagcttcttg
      241 cccaggtact ccggggtccg gccgaccagc agcccggcca ggaacaccgc gatcacggcc
      301 agcaccagga tgccgtagag gccggtcccg accccgcccg gcgcgacctc gccgagcaaa
      361 atgttcagca gcaccgctcc gccggcgccg ccggtgtagc tgtcgtgcat cgagttgacc
      421 gcgccggtcg acgtgccggt ggtggagatc ccgaacagga tcgacgacgg aatcccgaac
      481 cgcgtctcct tgccctccag tgccccgccg gcagcgagag tggcggcccc gttggggtgg
      541 gtctcggcga accaggcgac cgcgagcagc gcggcccaca gcacgcccat caccgagacg
      601 agcacggtgc cctgccgcgg gttgcgcacc atggtgccga acgtgcgggt caggcagacc
      661 gggatcacca gcagcaggaa gatctcgaac aggttcgaca gcccgttcgg gttctcgaac
      721 ggatgcgccg agttcgcgtt gaagatcccg ccgccgttgg tacccagctc cttgatcgcc
      781 tcctgcgacg ccgtcggcgc cagcggcagc gtgtgcacgg caccgtcgac gccggtgacg
      841 tcgaccccgg agcgcagcga catcgtcacc cccatcgcga tcagcaggat cgccgcgacg
      901 aaggagaagg gcagcaggat ccggacggca ccgcgggtca gatcgaccca gaagttcccg
      961 atccggtcgg tgcccgagcg gacgaacccg cggatcagcg cgacggcgac cgccatgccg
     1021 accgcggccg acagcacgtt ctgcaccgtc aggcccgcca tctgcaccgt gttgccaagc
     1081 acctgctccg gcacgtagga ctgccagttc gtgttcgtca cgaacgagac cgcggtgttg
     1141 aaggccatcg gcggggcgac cgcgccacgg ccgaaactca tcggcaggaa cggctgcagc
     1201 cgctgcaaca ggtacagcag caccaccgag acgaacgaga accccagcac ccccgccgcg
     1261 taggtgggcc agcgttgacc ggtgctcggg tcgacccgga ccagccggta gagcacggac
     1321 tcgacccgcc agtgacgttc cgaggtgaac gcacgggcca tgtagtcccc gaagggcacg
     1381 taacaggcca cgagcatgac caacagcagg acgacctgca gcagccccgc gacggtgtcg
     1441 ctcatcagaa cctctccggg cgcagcaggg caaccatcag gtagacgagg agtgccagcg
     1501 cgacgatccc gccgatcacg ttggtgacca cgtcagcggt gctcacagcc gctccagacc
     1561 acgcaggcac agcgccagca cgacgaaccc accgaccagc agcagcacgt acagcacatc
     1621 ggccacggac aactcctcga actcctggcc gccggacctc ccggcgaaac cgacgaaagt
     1681 gtggatccga ccgcagtgcc cgcgaagggg ttcgaacaca accttgacgc ccgcggcgga
     1741 aatcttgacg cgatcttggc gccgcggaga cacgcaccac cacggcgcac atcagcgctc
     1801 cgcaagcaca ggccaaccac ggacgtcaag aactcgttcg gatcgggtcg atcggcgtca
     1861 gggcttcgca ggatccctcc cccgaaccca ttgcacggcc cacgctcgaa ctgtgaacct
     1921 tccggatcct cgtgaggccc gcggccatcg gctcggtacg ccgttgtcca ctcgcggtgg
     1981 cttcctcgcc ggcttgatcg ccgccacggc ggcccccctt ctcgcctgga cgctcgacgc
     2041 ccgcgcagtc gccgtcggag gcctcgtcct ggccgcgctc gcggccgttc tgatcgccgc
     2101 gctgtcaacg accgccgcag cgatcgacac cgccgccgtg tgttgggccg cctacgacgg
     2161 attcgcgctg aaccgcctcg gcgagctgca cctggcagcc gccgatctcc gcgccctcgg
     2221 ctacatagtt ggcgctgcac tgagctttcg atgcctcgcc gccgcgctgc gcgccgtgcg
     2281 ggcggcggcc cgcacgagca cggagctctc gatccccctg cccgctgagc tcatcgccga
     2341 cctttccccg cgcccgaacc tgcggccgag cgcccatagt cgtcctcgcc gtcctgactc
     2401 tcggctacgg cgggatcgac gtcctcagaa cccgcaacct gctgtgcaag caccgatccc
     2461 aacggcaggc cctaccgacg gccagaccgg cgaccgatcc tggctccgca gtcaacgacg
     2521 gccacggacg caggcaccgg cccgatctgg tgctcttcat gctcctcaat tccttgatga
     2581 gaacctcatc gtgatgtcga aggagtgggc ttcaactcgg atggggcatc gggacgaagc
     2641 cgctcgtcct cgcccagctc ctcacacccc ggtcacgccg gccggtctgc actgcccggc
     2701 caggcaagaa gcgacgcgac gccgcccagc cgaacagctc gaggacgagc tccgaccgtg
     2761 tccgttgctc ggcatcgagc ttcgcggctt ccggtgaact gacgaagtcc gtagccacac
     2821 gagactcgcc ctgcgacggc ccgatgtcgt caatgacagc gcccatctgc gctagccgtc
     2881 agccgggctc tgggtgtacc tactagctgc aagtcagtcg gacggcgcca cgtgacagtc
     2941 gatgttgatc ttctcgtagc tcggagggtt aggaagcccg ccgaagtcgg ggacggccgc
     3001 gccgcggagt tgattgtccg caacccagcg catacctacg acgggtgtgg aggtcgttgt
     3061 aacgggcact cgaaccaggc tggccgccgt gcctcagcgg ctgtgcccgg tgttggtcgg
     3121 ccgccacggc gacctggacg ggtgtggttc gcctcgtggg ggccgagcgc ggtggcgtgg
     3181 cggtcgtcgt tggagaccga acggtcgagc accagccggt gcacaccacc ggctcacccg
     3241 cggagctcac tacgggggta accgagtggc tcgagcgcaa caagagtgat ggttacgctg
     3301 ccagcgcgca cgttgtcggc ggggcaaggc cagaccaacg gaggcagcgc atgggcacga
     3361 tcaccacgac cgacggcacc gagatcttct acaaggactg gggctcgggc ccaccgatcg
     3421 tcttcagcca cggctggccg ctttcgggtg acgattggga cacccaaatg ctgttcttcc
     3481 tccagcacgg c
//
LOCUS       DASYLX010000290         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2973631,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000290 DASYLX010000000
VERSION     DASYLX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973631"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VGP27_07080"
     CDS             complement(<1..918)
                     /locus_tag="VGP27_07080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851114.1"
                     /translation="MDFSPDEGQQAVADVVTSVLDRDNSWDALVAGGVTALALPERLG
                     GDGVGLSELSTALTEIGRHGTTGPALGVLGATAVLLDSASEPQQDRYLAGVAKGTIVT
                     LALNEPGAALPDKPATTLAGGKLNGTKIAVSYAGQADWLVVTADSGVVVVSAKADGVQ
                     ASKTPTSNHSDEYVVTFSDVTVVDDDVLDGAQAKRVNQLALATIGSFAAGLVAGALRL
                     TADYVANREQFGKPLSTFQTVAAQLAEVYIASRTLTLAATSVIWRLSEGRDADEDLDI
                     LGYWLASQAPPVMQLCHHMHGGMGMDIAYP"
     gene            complement(943..>1623)
                     /locus_tag="VGP27_07085"
     CDS             complement(943..>1623)
                     /locus_tag="VGP27_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851115.1"
                     /translation="PEYADIDPAQSSMELIMYAMKMAEERHQNPTEDIVTTLIKADVD
                     GEKLSDDEFGFFVIMLAVAGNETTRNSITHGMIAFANNPDQWELYKKERPETAADEII
                     RWATPVSAFQRTASEDTELGGVQIKKGQRVVMSYRSANFDEEVFEDPHSFNILRDPNP
                     HVGFGGTGAHYCIGANLARMTINLIFNAVADHMPDLKPVGVPERLRSGWLNGIKHWQV
                     DYTGATAH"
BASE COUNT          259 a          537 c          525 g          302 t
ORIGIN      
        1 cgggtacgcg atgtccatgc ccatgccgcc gtgcatgtgg tgacacagct gcatcacggg
       61 cggcgcctgc gacgcgagcc agtaacccag aatgtcgagg tcttcgtcgg cgtcgcgtcc
      121 ctccgataac cgccatatca ccgaagtcgc cgccaacgtc agtgtgcgcg aagcgatgta
      181 cacctcggcc agctgcgcgg ccaccgtctg gaacgtcgag agcggcttgc cgaactgctc
      241 gcggttggcg acgtagtcgg cagtcaggcg caacgccccg gccaccagcc ctgccgcgaa
      301 cgagccgatc gtcgcgagcg ccaactgatt gacccgcttc gcctgcgcgc cgtccagcac
      361 gtcgtcgtcg acgaccgtga catccgagaa cgtcaccaca tactcatcgg agtgattgga
      421 cgtcggcgtc ttggaggcct gcacaccatc ggccttcgcc gacacgacga ccacaccgct
      481 gtcggccgtg acgacgagcc agtccgcctg tcccgcatag gaaacggcga tcttcgtgcc
      541 gttgagcttg ccgcccgcga gtgtcgtcgc cggtttgtcc ggcagcgccg cacccggttc
      601 gttgagggcg agcgtcacga tcgtgccctt tgcgacgccg gccaggtacc ggtcctgctg
      661 gggctcggac gctgaatcga ggagcacggc ggtggccccg agaacgccca gcgcaggtcc
      721 ggtggtgccg tgcctgccga tctcggtcag cgccgtggag agctcggaca gcccgacacc
      781 gtcaccgccg aggcgctccg gcagggccag cgcagtgacg ccacccgcga ccagagcgtc
      841 ccacgagttg tcgcggtcca gcaccgaggt gacgacgtcg gcgacagcct gctgcccttc
      901 gtccggactg aaatccaccc gaagcctcct tttatcgcgg gctcagtgcg cagtggcgcc
      961 ggtgtagtcc acctgccagt gcttgatgcc gttgagccat cctgaccgta gccgctcggg
     1021 tacaccgacc ggcttgaggt cgggcatgtg gtctgcgacg gcgttgaaga tcaggttgat
     1081 cgtcatccgg gcgaggttcg caccgatgca gtaatgcgca cccgtgccac cgaagccgac
     1141 gtgcggattc gggtcgcgca ggatgttgaa cgagtgcggg tcctcgaaga cctcttcatc
     1201 gaaattggcc gagcggtaag acatcaccac ccgctggccc ttttttatct gcacgccgcc
     1261 aagctcggtg tcctcgcttg cggtgcgttg gaatgccgac accggcgtgg cccatcggat
     1321 gatctcgtcg gcggcggttt cgggacgctc cttcttgtag agctcccact ggtcggggtt
     1381 gttcgcgaag gcgatcatgc cgtgggtgat cgagttgcgg gtggtctcgt tgcctgccac
     1441 cgcgagcatg atgacgaaga agccgaactc gtcgtcggag agcttttcgc catcgacgtc
     1501 ggccttgatc agcgtcgtga cgatgtcctc ggtggggttt tggtgccgct cctcggccat
     1561 cttcatcgcg tacatgatca gttccatcga cgactgcgcc ggatcgatgt cggcgtactc
     1621 ggg
//
LOCUS       DASYLX010000291         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_122097,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000291 DASYLX010000000
VERSION     DASYLX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122097"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VGP27_07090"
     CDS             <1..133
                     /locus_tag="VGP27_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7851116.1"
                     /translation="KRLMNIQLEQAVMASLDYANLAEYVSFGTADFNKIVDGLIAKK"
     gene            513..1451
                     /locus_tag="VGP27_07095"
     CDS             513..1451
                     /locus_tag="VGP27_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7851117.1"
                     /translation="MTAATDIALQVTPLSGWTGALVTGVDLSVPLSDDARTAIKDALH
                     QWKVVFFRDQKLDHAAQIAFGAQFGEVTPGHPYEGDSAPPDFPQIHTVSPAAYDRRYG
                     TRYRKKQSPNGPKWHADVTPLINPPSHSILRAEAVPPYGGDTQFVNVAAVYQGLSESV
                     RAFIDELRAEHRFGADVAADRSSEKVGDLVRTNPLASIHPVVRVHPDTGERVLYVNPS
                     FTRHIVDLAPRESRHVLDLLFEEIGRPEYSVRFKWAPGSVAFWDNRAALHLAPQDFEH
                     VEGDRVLHRITLVGDVPVGPSGQPSESIAGEFFGAA"
     gene            1453..2325
                     /locus_tag="VGP27_07100"
     CDS             1453..2325
                     /locus_tag="VGP27_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851118.1"
                     /translation="MATVVVRPRAAAGASAKADGGALVDPRAVFGKRSRRQFVPRPVQ
                     RLLGPVLLVALWQILSATGVFDERTVPPPTRVVSAGLRLIADGSLQEHLFTSLLRVGY
                     GLVFGVVLGLSLSLIAGLSRVGENFVDANMEVLRAVPNFALVPLLIVWFGISEVPKIT
                     LITLAVAVAIYINTFSAIRSVDAGLVEAAKSFGAGRVELIYRVIIPGSLPGFLVGLRL
                     ALTASWLSLIFAETINAKKGLGRMMTDAREYFQIDVVFVLIAVYAILGLVSILVVRFL
                     ETRLLTWRRAYDGD"
     gene            2312..3043
                     /locus_tag="VGP27_07105"
     CDS             2312..3043
                     /locus_tag="VGP27_07105"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851119.1"
                     /translation="MTATELGTDTVVTARDIRKRFGDNVVLDGLDLNIGAGEFVAMLG
                     RSGSGKSTFLRALSALDGDFDGELRVPERRAIVFQDPRLLPWQRVLTNVTVGLPTTAA
                     VRKQARVALDEVSLTDKAKVWPRTLSGGEAQRVALARALVREPELLLLDEPFGALDAL
                     TRIKMHALLLALCRRHKPAVLLVTHDVDEAILLADRVIVLTDGAISLDIEVTVAAPRV
                     RDSHEFATLRRRLLAELGVTGEVQL"
     gene            3076..4146
                     /locus_tag="VGP27_07110"
     CDS             3076..4146
                     /locus_tag="VGP27_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEV7851120.1"
                     /translation="MWRTPRARWKLTALVAISLLTAACGLSDDEDNSAIDSVKIASRT
                     DVPAGTKLVIAEQNGTQSLPWKLANAGKDAPYSVEFADFSGGAAVIEALRSGAADVGS
                     IGEAPVPIAVDSGVTDLVTVGLQANPGTSGGYFLVARPNSGITKIEDLRGKRVAYPPG
                     SGRHMVTAALLKQHGLNLKHDVQPVELAGSEVVPTFAAGAVDAAIVLGNQYYQLGEPP
                     ILGDGTGINTGIQSVIVRKDILDDPAKVAAIGDYVGRAVAANNWKDSHVDQWIDEYYV
                     KQQGITFDQGKKLYEEDGLAAYYPIDGASTGLFQKVADGLYDTGTTKTHVDIKPYVDG
                     RYNDIVNAQNQLDGVTPKPINA"
BASE COUNT          750 a         1336 c         1391 g          792 t
ORIGIN      
        1 caagcggttg atgaacatcc aactcgagca ggccgtgatg gcgtcgctgg attacgccaa
       61 cctcgccgag tacgtgtcct tcggcaccgc cgacttcaac aagatcgtcg acggcctgat
      121 cgccaagaag taactcgcct cttccctccg cgagctgggg gcccgtttcg ccccgcgacg
      181 gtgcgtgtct gctgcccgac acgccgtcgt tagtgcgcaa tttgtgcgcg ctcgctaagc
      241 cgcggcggtg cagatacggc cggtcttctt cggcgtggct ggcgacaaac gcgtagcgcg
      301 gtggaacgag tgcttgtaca acggcggtct gcgatcgctc gggcgcgatg agcgcgcgcc
      361 tatgtacgcg tctcgcggcg tgtcggcgta caaagacgcc ggctcgcggt ggggcgattg
      421 catcacgtcg attcaaaccc gtatcggatc agcgcatcgc cgataacgtc gcattcgttg
      481 atcttgcaat cactttcgaa ggggaccgat tgatgaccgc tgcaacggat atcgcactgc
      541 aggtgacgcc gctgtcgggg tggactggcg cattggtcac cggcgtcgac ctttccgtgc
      601 cgctgtccga cgacgccagg accgctatca aggacgcgct tcatcagtgg aaggtggtgt
      661 tcttccgcga tcagaagctc gaccatgctg cgcagattgc gttcggcgcg cagttcggtg
      721 aagtgacccc gggacacccc tatgagggtg attcggcacc gccggacttc ccgcagattc
      781 acacggtgtc gccggcggcg tatgaccgcc gctacggcac cagataccgt aagaagcaga
      841 gtccgaacgg acccaagtgg cacgccgatg tcacgccgct gatcaacccg ccctcgcatt
      901 cgatcctgcg cgccgaagcg gtgccgccgt atggtggcga cacccaattc gtcaacgtcg
      961 ccgcggttta ccaaggactt tccgaatccg tccgcgcgtt catcgacgaa ctccgcgccg
     1021 agcaccgctt cggtgcagac gtcgccgcgg accgcagctc ggagaaggtc ggcgacctgg
     1081 tgcgtaccaa tccactggcc tcgattcatc ccgttgtccg ggtgcatccg gacaccggcg
     1141 aacgcgtgtt gtacgtcaac ccgtccttca cgcgtcacat cgtcgacctg gcgccgcgcg
     1201 agagccgtca cgtcctggat ctgctgttcg aggagatcgg ccgccccgaa tattcggtcc
     1261 ggttcaagtg ggcgcccggc agcgtggcgt tctgggacaa ccgcgccgcc ctgcatctcg
     1321 ccccccagga cttcgagcac gtcgagggcg atcgtgtact gcaccgcatc actttggtgg
     1381 gcgatgtgcc ggtcggcccg tccggccagc cgtcggagtc gatcgcaggc gagttcttcg
     1441 gggcagcttg agatggcgac cgtggtagtc cgcccgcggg cggccgcggg cgcgtcggcc
     1501 aaagcggacg gcggcgcact tgtcgacccg cgtgcggtgt tcggcaagcg cagtcgacga
     1561 cagttcgtgc cacgcccggt gcagcggctg ctgggcccgg tgctgctcgt tgcgttgtgg
     1621 cagatcttgt cggccactgg cgttttcgac gagcgcacgg tgccgccacc gacgagggtg
     1681 gtcagcgcgg gccttcggct catcgccgat ggctcgctcc aggagcatct gttcaccagc
     1741 ttgctgcggg tcggctacgg cctggtgttc ggggtggtgc tcggtttgag cctgtcgctg
     1801 atcgcaggtc tgtcgagggt gggggagaac ttcgtcgacg cgaacatgga agtgctgcgt
     1861 gcagttccca acttcgccct cgtgccgctg ctcatcgtct ggttcggcat cagcgaggtg
     1921 ccgaagatca cgctgatcac actcgcagtg gcggtcgcca tctacatcaa cacattcagc
     1981 gccattcgca gcgtggacgc cggattggtc gaggcggcaa agtccttcgg tgccggccgc
     2041 gttgaactga tctaccgcgt catcattccg ggttcgctgc ccggattcct cgttgggctt
     2101 cggcttgcgc tcaccgcgtc atggctgtcg ctgatcttcg ccgaaacgat caacgcgaag
     2161 aaggggctcg gccggatgat gaccgacgct cgcgagtatt tccagatcga cgtcgtgttc
     2221 gtgctcatcg cggtgtacgc gatcctgggg ctggtgtcga tacttgtcgt gcggttcctt
     2281 gagacgcgac tgctcacctg gaggcgggcg tatgacggcg actgagctcg ggaccgacac
     2341 ggtcgtcacg gcccgcgata ttcggaaaag gttcggtgac aacgtcgttc tggacggact
     2401 tgacctaaac attggtgcgg gtgaattcgt cgccatgctg ggccgcagcg gttcgggcaa
     2461 gagcacgttt ctgcgcgcgc tcagcgcact ggacggcgac ttcgacggag aacttcgggt
     2521 gcccgagcgg cgggccatcg tgttccaaga tccgcgactg ttgccgtggc aacgtgtgct
     2581 gaccaacgtg accgtcggtt tgccgacgac ggccgcggtc cggaagcagg cgagggttgc
     2641 tctcgacgag gtgagcctga ccgacaaggc caaggtgtgg ccacggactt tgtcaggggg
     2701 agaggcgcag cgtgttgcgc tggcccgggc attggtacgc gagcccgaat tgttgcttct
     2761 ggatgagcct ttcggggctc tggatgccct cacccgaatc aagatgcatg ccctgctgct
     2821 cgcactctgc cgacgccaca agccagccgt cttgttggtc actcacgacg tcgacgaagc
     2881 gattctgctg gccgaccggg tgatcgtgct caccgacggc gccatctccc tcgacatcga
     2941 ggtgactgtg gcggcgccga gagtccggga ctctcacgaa ttcgcaacac tgcgacgtcg
     3001 cttgctcgcc gaactcggcg tcaccggaga ggttcaactg tgaatcgtgc tagctcacct
     3061 gacgaaggac aacgaatgtg gagaactccg cgcgcgcggt ggaagttgac tgctctggtt
     3121 gcgatctcgc tcctgaccgc cgcctgtggg ctgtcggatg acgaggacaa cagcgcgatc
     3181 gattcagtca agatcgcctc gagaactgac gtgcctgccg gcacaaagct cgtcatagcc
     3241 gaacagaacg gcacgcagtc gctgccgtgg aagcttgcca atgccggaaa ggatgctccc
     3301 tattcggtcg agttcgcgga cttcagtggc ggcgcggcgg tcatcgaggc ccttcggtcg
     3361 ggtgccgccg atgtcggttc catcggcgag gcgccggtgc cgatcgccgt cgacagtggc
     3421 gttaccgacc tggtcaccgt tgggctgcag gcgaatccgg gcaccagcgg tggatacttt
     3481 ctggtggcca ggccgaacag cggtatcacc aagatcgagg acctgcgcgg caagcgggtc
     3541 gcctatccgc cgggctccgg ccgccatatg gtcaccgcgg cgctgctcaa acagcacgga
     3601 ctgaacctca agcacgatgt tcagccggtg gaactggcgg gctccgaggt ggttccgacg
     3661 ttcgcggccg gtgccgtcga tgcggccatc gtgctgggca accagtacta ccagctcggc
     3721 gaaccgccga tcctcggtga cggaacggga atcaacaccg gaatccaaag cgtgatcgtt
     3781 cgcaaggaca tcctcgacga cccggcgaag gtggctgcga tcggtgacta cgtgggccgc
     3841 gccgtcgccg cgaacaactg gaaggactcg cacgtcgatc agtggatcga cgagtactac
     3901 gtgaagcagc agggcatcac cttcgatcag ggcaaaaagc tctacgaaga ggacggcctt
     3961 gcggcctact acccgatcga cggcgcctcc acgggcctct tccagaaagt cgccgacggg
     4021 ttgtacgaca ccgggaccac gaagacacac gtcgacatca agccgtacgt cgacggccgc
     4081 tacaacgaca tcgtcaacgc acagaatcag ctcgacggcg tcacacccaa accgatcaat
     4141 gcatgacgaa agagggggat tgacatcatg actcaggtag cgcaggcgct cgaggtgagg
     4201 ccgcagtccg gttggacagg tgccgagatc cacggcatcg acctgaccaa gccgctcgat
     4261 gatcagcag
//
LOCUS       DASYLX010000292         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_340898,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000292 DASYLX010000000
VERSION     DASYLX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340898"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..885)
                     /locus_tag="VGP27_07115"
     CDS             complement(157..885)
                     /locus_tag="VGP27_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEV7851121.1"
                     /translation="MDPLAGFPVTAPALARPVTFDQFWADLTFLHWPVQPDAVAHLYP
                     PGTRPDVFDGLTYAGLVPFAMRSTAIGTSLPLPYFGTFHETNIRLYSIDDAGRHGVVF
                     RSLETARLAVVPLVRAALGVPYTWARMRVTRSHGRICYESERRWPRRGLHSRLIARIG
                     DEVEPTPLEIWLTARWGAHTRKAGRTWWMPNEHGPWPLQSAEILELDDDLMEAAGIQV
                     AGGRLRALFSPGVHARFGRPVRVK"
     gene            912..1700
                     /locus_tag="VGP27_07120"
     CDS             912..1700
                     /locus_tag="VGP27_07120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851122.1"
                     /translation="MSTSQSAPTTWSSGRYDAIGDHIAGIAADVVAAAKRRGPFRDVA
                     DLACGTGSAALVAAAAGARVTAVDVTGALIAIGRQKAERLGHTVNWVTADASDTGLPA
                     GAFDAVVSNMGIIFVEPVAQVAEIARLLKAGGVVGFSSWVPDPQTPFYKPIVTVLGSP
                     PASGHSPDQWGTTETITDRLAPDFDDIAIETGACRWRLGTIEDAMHLLADESPIHVSL
                     LDNLEAAKRDELLAAFEDAMRANIGEDGLVAFDAPYLVVTALRR"
     gene            complement(1704..2462)
                     /locus_tag="VGP27_07125"
     CDS             complement(1704..2462)
                     /locus_tag="VGP27_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEV7851123.1"
                     /translation="MCGRFAVTTDPALLAEKIQAIDESTAAVEGKGGGGPNYNVAPTT
                     TISTVVKRHSEPDDEPTRRVRSMRWGLVPPWAKEAESGVPDTKGPLLINARAEKVTTS
                     PAFRNSAKNKRCLVPMDGWYEWRGEKGSKTPFYMYGGDGEPLFMAALWSTWRPKGAPK
                     DSPPLLSTTIITTDSAGPLAEIHDRMPLTISAGDWDRWLDPDAAIDEGLLRGHGDLDR
                     IEIREVSRLVNSIRNNGPELIEPAEPEPRQATLL"
     gene            2570..3838
                     /gene="aroA"
                     /locus_tag="VGP27_07130"
     CDS             2570..3838
                     /gene="aroA"
                     /locus_tag="VGP27_07130"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV7851124.1"
                     /translation="MWQAPTTPTPVHATVTVPGSKSQTNRALVLAALATAQGTSTISG
                     ALRSRDTDLMIGALRTLGVTVDGDDTELTVSGRIAPPADATVDCGLAGTVLRFLPPVA
                     ALSTQTVVFDGDEQARARPIAPLLDALRGLGVDIDGAGLPFSVRGGGTVAGGTVEIDA
                     SASSQFVSGLLLSGAMFTDGLTIVHTGESVPSAPHVAMTVAMLRDAGVDVDDTQTNRW
                     RVSPGPVSARHWVIEPDLSNSVPFLAAAVVSGGIVRIAGWPAASTQPADTILAILEKL
                     GSIVRRSSSYLEVQGSQAYGGFDVDLHDVGELAPAVAALAALATPGSVSQLTGIAHLR
                     GHETDRLAALSAEINGLGGQCEEIPDGLVVTARQLHGGLWRSYADHRMATAGAIVGLR
                     VPGVEVEDIGTTAKTLPEFPQMWAGMLAGQ"
     gene            3880..>4515
                     /gene="rsgA"
                     /locus_tag="VGP27_07135"
     CDS             3880..>4515
                     /gene="rsgA"
                     /locus_tag="VGP27_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HEV7851125.1"
                     /translation="MSTRDYDESDVRIRPGRGSRPRTKTRPEHADAQEGMVVTVDRGR
                     WGCVLGRDPGRRVTAMRARELGRTPIVVGDDVGLVGDLSGRTDALARIVRRGERRTVL
                     RRTADDTDPTERVVVANADQLLIVVALADPPPRTGFVERALIAAYAGGLTPILCLTKS
                     DLAPAGPFAAQFADLDLTIAIAGRDDPLDAVAPLLTDKVTVLLGHSGVGKST"
BASE COUNT          667 a         1556 c         1597 g          695 t
ORIGIN      
        1 ggcggccacg gtttcggccg agtcgttcga tactgcgccg acgacggtgc cgtcggcagg
       61 gttgcgaact tcgatggtgg tgagctgcgg cttggtctgc agatcggtca tgggcaaagc
      121 gtgctccaac cggaggcccg atttgcgcgt attcgactac ttcacccgca ccggacgccc
      181 gaaccttgcg tgcacgccgg gtgagaagag cgcgcgcagc cggccacccg cgacctgaat
      241 tcccgcggcc tccatcagat cgtcgtccag ctcaagaatc tcggccgatt gcagcggcca
      301 cggaccgtgt tcgttgggca tccaccatgt ccggccggcc ttgcgggtgt gtgcacccca
      361 tcgggcggtc agccagatct ccagcggcgt cggctccacc tcgtcgccga tccgcgcgat
      421 cagccggctg tgcaggccgc ggcgcggcca gcggcgctcg ctctcgtagc agatgcggcc
      481 gtgggatcgg gtgacgcgca tccgtgccca ggtgtacggg acaccgagcg cggcgcggac
      541 gagaggcacc acggcaagcc gcgcggtttc caaggaccgg aagaccacgc cgtggcggcc
      601 ggcatcgtcg atcgaataca gcctgatgtt ggtctcgtgg aaggtgccga agtacggcag
      661 cggcagcgac gtgccaatcg ccgtgctgcg catggcgaac ggcaccagcc cggcgtaggt
      721 gaggccgtcg aacacgtcgg gtcgggttcc cggcgggtac agatgcgcga cggcgtccgg
      781 ctgcaccggc cagtgcagga aggtcaggtc ggcccagaac tggtcgaacg tcaccggacg
      841 cgcaagggcc ggtgcggtga cggggaaccc ggccagtggg tccatctcga catcgtgaca
      901 cgctgagccc cgtgtccacc tcgcagtccg ccccgacgac gtggtcctcc ggtcgctacg
      961 acgcgatcgg cgatcacatc gccggaatcg cggcggacgt cgttgccgcc gcgaaacgac
     1021 gcggaccgtt ccgtgacgtg gccgaccttg cttgcggcac cggcagcgcc gcactcgtcg
     1081 cagcggcggc gggtgcccgg gtgacggcgg tcgacgtcac tggcgcgttg attgcgatcg
     1141 gccgacagaa ggccgagagg ttggggcaca ccgtgaactg ggtgacggcc gacgcctccg
     1201 acaccggcct gccggccgga gcgttcgacg ccgtggtgtc gaacatgggc atcatcttcg
     1261 tcgagcccgt cgcacaggtg gccgagatcg cgcggctgct caaggcgggc ggtgtggtgg
     1321 gcttctcttc gtgggtgccc gacccgcaga ccccgttcta caagccgatc gtgaccgtgc
     1381 tgggatcacc acccgcctcg gggcactccc ccgatcagtg gggaacaacc gaaacgatca
     1441 ccgaccggct cgccccggac ttcgacgaca tcgcgatcga aacaggcgcc tgcagatggc
     1501 gattgggaac catcgaggac gccatgcacc tgttggcgga tgagtcgccg atacatgtct
     1561 ccctgctcga caacctcgaa gccgccaagc gcgacgagct gctggctgcc ttcgaggacg
     1621 cgatgcgcgc caacatcggc gaggacggcc tcgtcgcgtt cgacgcgccc tacctggtgg
     1681 tgacggccct gcggcggtga cgcctacagc agcgtggcct gcctgggttc cggctcggcc
     1741 ggttcgatca gctcgggccc gttgttgcgg atgctgttca ccaaccgcga cacctcgcgg
     1801 atctcgatgc ggtcgaggtc gccgtgcccg cgcagcagcc cctcgtcgat ggctgcatcg
     1861 gggtcgagcc accggtccca gtcgccggcg ctgatggtca aaggcatccg gtcgtgaatc
     1921 tcggcgagcg gacctgccga gtcggtggtg atgatcgtgg tgctcagcag cggcgggctg
     1981 tccttcggcg cgcccttcgg ccgccacgtc gaccacagag ccgccatgaa cagcggctct
     2041 ccgtcgccgc cgtacatgta gaagggcgtc ttcgagcctt tctcgccgcg ccactcgtac
     2101 cagccgtcca tcgggaccag gcagcgcttg ttcttcgccg agttgcggaa cgcgggtgag
     2161 gtggtgacct tctcggcgcg tgcgttgatc agcagcggcc ccttggtgtc ggggacaccg
     2221 ctctcggcct ccttggccca cggcggcacc aggccccagc gcatcgagcg gacgcgccgg
     2281 gtcggctcgt cgtcgggctc gctgtgacgc ttgaccaccg tgctgatcgt ggtggtcggc
     2341 gcgacgttgt agttcggccc gccgccgccc tttccctcga ccgccgcggt gctttcgtca
     2401 atggcctgga tcttctcggc cagcagcgcc ggatcagtgg taaccgcgaa tcgcccacac
     2461 atacatccca tggtggcaga aagctgtgac agggcaggat agggccgtaa gcaaccgcgc
     2521 gcgggtggga gtaccttcca cccgaagggt agggggagag cacaggaaca tgtggcaggc
     2581 accgacgacc ccgactcccg tgcatgccac ggtgacggtg ccgggctcga aatcgcagac
     2641 caaccgcgcg ctcgtgctgg cggccctggc aactgcgcag ggcacgtcga cgatcagcgg
     2701 tgcgctgcgc agccgcgaca ccgacttgat gatcggggcg ctgcgcacgc tgggggtcac
     2761 cgtcgacggc gacgacaccg agctgacggt cagcggccgg atcgccccgc ctgccgacgc
     2821 gaccgtcgac tgcggcctgg cgggcacggt gctgaggttc cttccgccgg tcgcggcgct
     2881 ttctactcag acggtggtgt tcgacggcga cgaacaggcc cgagcccgcc cgatcgcgcc
     2941 gctgctggac gcactgcgcg gcctcggtgt ggacatcgac ggcgccggcc tgccgttctc
     3001 ggtgcgcgga ggcgggacgg tggccggcgg caccgtcgag atcgacgcct cggcgtcgtc
     3061 gcagttcgtc tccgggttgc tgctgtccgg cgcgatgttc accgacgggc tgaccatcgt
     3121 gcacaccggc gagtcggtgc cgtcggcgcc gcacgtggcg atgacggtgg cgatgctgcg
     3181 cgacgcgggc gtcgacgtcg acgacaccca gaccaaccgc tggcgggtct cccccggtcc
     3241 ggtctccgca cggcactggg tgatcgagcc cgacctgtcc aattcggtgc cgttcctcgc
     3301 cgccgcggtc gtcagcggcg ggatcgtgcg gatcgccggc tggcctgcgg ccagcactca
     3361 gcccgccgac accatcctcg ccattctcga gaagctggga tcgattgtgc ggcgcagtag
     3421 ttcgtacctc gaagtgcagg gcagccaggc gtacggcggg ttcgacgtcg acctgcacga
     3481 cgtcggcgag ctggcgcccg cggtcgcggc gctggccgcg ctggctacgc cgggttcggt
     3541 gtcgcagctg accggcatcg cccatctgcg cggccacgag accgaccggc ttgcggcgct
     3601 gagcgccgaa ataaacggcc tcggtgggca gtgcgaggag attcccgacg gcctggtcgt
     3661 caccgcccgg cagctgcacg gcggcctgtg gcggtcctac gccgaccatc gaatggcgac
     3721 ggccggcgcg atcgtcgggc tgcgggtacc cggcgtcgag gtggaagaca tcggcaccac
     3781 ggccaagacc ctgccggagt ttccgcagat gtgggccggc atgctggccg gccaatagac
     3841 gatgtctgcg gcccgggacg cgagggggat tcgaaggcgg tgagcacgcg ggactacgac
     3901 gaatccgacg tccggatccg gcccgggcgg ggctccaggc ccaggacgaa gacccgcccc
     3961 gagcacgccg acgcccagga gggcatggtc gtgacggtcg atcgcgggcg gtggggttgc
     4021 gtgctcggcc gcgaccctgg ccgtcgggtg accgccatgc gggcccggga actgggccgc
     4081 actccgatcg tggtcggcga cgacgtcggc cttgtgggtg acctgtcggg gcgcacggac
     4141 gcgttggccc gcatcgtgcg gcgcggagaa cgacgaacag tgttgcggcg caccgcagat
     4201 gacaccgatc caacggagcg ggtcgtcgtc gccaacgcgg atcagctgtt gatcgtggtc
     4261 gcactggccg acccgccgcc gcgcaccggc ttcgtcgaac gcgcgctgat cgccgcctac
     4321 gcgggcggcc tcaccccgat cctgtgcctg accaaatccg acctcgcgcc cgcagggccg
     4381 ttcgccgcgc agttcgccga cctcgatctg accatcgcga tcgccggccg cgacgatccg
     4441 ctcgacgccg tcgccccact gctcaccgac aaggtgaccg tgctgctcgg ccactccggc
     4501 gtcggaaagt ccacg
//
LOCUS       DASYLX010000293         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3084185,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000293 DASYLX010000000
VERSION     DASYLX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084185"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2238)
                     /locus_tag="VGP27_07140"
     CDS             complement(<1..2238)
                     /locus_tag="VGP27_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7851126.1"
                     /translation="MPKVVVIGAGVVGAALADELTGRGLTDLTVLDRGPLFATGGSST
                     HAPGLVFQTNPSKTMSAFAGYTIDKFCSLEHRDGWVFNQVGGLEVATTPERWADLHRK
                     IGWAEAWGIEGRLLSPAECVRLHPLVDVDRILGGLHTPADGLAKAVRAVEAQAGRAMA
                     RGATFVPHTEVVGIVDKGGRVVGVRTADGVVDADIVVCAAGFWGAQLAKEVGLVVPLV
                     PMAHQYARTGQIAALVGRNTESSEAGLPILRHQDQDVYFREHVDRLGIGSYSHRPMPV
                     DMATLMADTAGEPMPSMLPFTEEDFAPAWSAAAELIPALGDSKVEEAFNGIFSFTPDG
                     FSIMGEHRDLRGFWVAEAVWVTHSAGVAKAMAEWIVDGTPSIDIHECDLYRFEDVARS
                     PDFIMQTSSQAFVEVYDVIHPSQFRTALRGLRTSPFYERQKELGAFFYEGGGWERPAW
                     FEANAELAQRLRADGLPFPQRDDWSSQFWSPISIAEAHWTREHVAMYDMTPLTRYEIA
                     GTGAAAFLQRMSTNNVDKSVGSVTYTMLLDETGGIRSDITVARLAHDRFQVAVNGPMD
                     FDWLSRHLPGDVTLRDITGGTCCVGAWGPLARDMVAPLCPDDLAHDRFKYFRALQTYL
                     GTIPVTMLRVSYVGELGWEIYTSAEYGAALWDLLVEAGRPHGVIAAGRIAFNSLRIEK
                     GYRLWGTDMTAEHLPAAAGVDFAVKMTKDDFVGKAALERAAAPQKVLRSIVFDEPTAV
                     VLGKEP"
     gene            complement(2393..3115)
                     /gene="prcA"
                     /locus_tag="VGP27_07145"
     CDS             complement(2393..3115)
                     /gene="prcA"
                     /locus_tag="VGP27_07145"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEV7851127.1"
                     /translation="MSFPYFISPEQAMRERSELARKGISRGRSVVVLAYDSGVLFVAE
                     NPSRSLQKVSELYDRVGFAAVGRFNEFDNLRRGGIQFADTRGYAYDRRDVTGRQLANV
                     YAQTLGTIFTEQAKPYEVELCVAEVAHFGETKAPELYRITYDGSIADEPHFVVMGGTT
                     EPIIEKLKDSYAENTGLSEAVKIAVAALKAGGNGSEDRVLGPDTLEVAILDTNRPRRA
                     FRRITGPALEAVLPEDSTSSEE"
     gene            complement(3112..4041)
                     /gene="prcB"
                     /locus_tag="VGP27_07150"
     CDS             complement(3112..4041)
                     /gene="prcB"
                     /locus_tag="VGP27_07150"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEV7851128.1"
                     /translation="MSWTQRDQLHFPSPLPGAPSVPQAFYAPHVRVDLSSFSELLRRS
                     APELLPVNRTGAEGIGAGDALPHGTTIVALTYPGGVVMAGDRRATQGNMIASRDVKKV
                     YITDDYTATGIAGTAAIAVEFARLYAVELEHYEKVEGVPLTFPGKVNRLATMVRGNLG
                     AALQGFIALPLLAGYDLDDPDPEAAGRIVSFDAAGGWNFEEEGYQSVGSGSIFAKSSI
                     KKLYSRVVDAESALRVAIEGLYDAADDDSATGGPDLVRGIYPTAVTLGAEGAEEVPEQ
                     RIAALAREVIESRSREDTFRTTSGPSTDARGDS"
     gene            complement(4038..4232)
                     /locus_tag="VGP27_07155"
     CDS             complement(4038..4232)
                     /locus_tag="VGP27_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEV7851129.1"
                     /translation="MAQEQTKRGGGGGEDDDPTGSTAAGQERREKLTEETDDLLDEID
                     DVLEENAEDFVRAYVQKGGQ"
     gene            complement(4324..>4745)
                     /locus_tag="VGP27_07160"
     CDS             complement(4324..>4745)
                     /locus_tag="VGP27_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEV7851130.1"
                     /translation="DWPAKLRLLEGFRNRENLNWSAPRLHLVDLQYSDVRLDKGLYNR
                     LVARGSMKRLTTEQQVRDAVDNPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGD
                     SLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTN"
BASE COUNT          776 a         1595 c         1571 g          803 t
ORIGIN      
        1 cggctccttg ccgagcacga cggcggtcgg ttcgtcgaac acgatgctgc gcaacacctt
       61 ctgcggtgcc gccgcgcgct ccagcgccgc cttgccgacg aagtcgtcct tggtcatctt
      121 gaccgcgaag tccacgcccg ccgcggcggg caggtgctcg gcggtcatgt cggtgcccca
      181 cagccggtaa cccttttcga tgcgcaggct gttgaacgcg atccgccctg cggcgatcac
      241 gccgtgtggc ctgcccgcct cgacgagcag atcccacagc gcggcaccgt attcggcgct
      301 ggtgtagatc tcccagccga gttcgccgac ataggagacc cgcagcatgg tgacggggat
      361 cgtgccgagg taggtctgca gcgcgcggaa gtacttgaat ctgtcgtgcg ccaggtcgtc
      421 ggggcacagc ggcgccacca tgtcgcgggc cagggggccc cacgcgccga cgcagcaggt
      481 gccgccggtg atgtcgcgca gcgtgacgtc accaggtagg tggcggctga gccagtcaaa
      541 gtccatcggc ccgttgacgg cgacctggaa ccggtcgtgc gcgagcctgg cgacggtgat
      601 atcgcttcgg attccgccgg tttcgtcgag cagcatcgtg taggtgaccg atccgacgct
      661 cttgtcgacg ttgttggtgg acatccgctg caggaacgcc gccgcgcccg tgcccgcgat
      721 ctcgtagcgg gtcagcggcg tcatgtcgta catcgcgacg tgctcgcggg tccagtgcgc
      781 ctcggcgatc gagatcggcg accagaactg tgacgaccag tcgtcgcgct gcgggaacgg
      841 cagaccatcg gcgcggagtc gttgtgccag ttcggcgttc gcctcgaacc aggccggccg
      901 ctcccaaccc ccaccttcgt agaagaacgc gccgagctcc ttctgccgct cgtagaacgg
      961 gctggtgcgc agcccgcgga gcgcagtgcg gaactgggag gggtggatga cgtcgtagac
     1021 ctcgacgaag gcctgcgagc tggtctgcat gatgaaatcg gggctgcggg ccacgtcctc
     1081 gaagcgatag aggtcgcatt cgtggatgtc gatcgacggt gttccgtcga cgatccattc
     1141 cgccatcgcc ttggcgacac ctgcggaatg cgtcacccac accgcttcgg cgacccagaa
     1201 gcccctcagg tcgcgatgct cacccatgat agagaaaccg tcgggcgtga aggagaagat
     1261 accgttgaac gcttcttcga ccttcgagtc cccaagtgcc ggaatgagtt cggctgcggc
     1321 ggaccaggcg ggcgcgaagt cctcctcggt gaagggcagc atcgacggca tcggctcgcc
     1381 tgccgtatcc gccatcaggg tggccatatc gactggcatc ggccggtggc tgtacgagcc
     1441 gatacccagg cggtcgacgt gctcgcggaa atagacgtcc tgatcttgat ggcgcaggat
     1501 cggaaggccg gcctcggacg actcggtgtt ccggcccacc agcgcggcga tctgtccggt
     1561 gcgcgcgtac tggtgcgcca tcggaaccag tggcaccacg aggccgacct ccttggccag
     1621 ctgggcgccc cagaacccgg ccgcacacac cacgatgtcg gcgtcgacga cgccgtcggc
     1681 cgtgcgcaca ccgaccaccc ggccgccctt gtcgacgatt ccgacgacct cggtgtgcgg
     1741 cacgaacgtc gcgccgcgcg ccatcgcgcg accggcctgg gcctcgacgg cgcgcaccgc
     1801 cttggcgagc ccgtcggcgg gtgtgtggag acccccgagg atgcggtcga catcgaccaa
     1861 cggatgcagc cggacgcatt cggcggggct gagcagcctg ccctcgattc cccaggcttc
     1921 ggcccagccg atcttgcggt gcaagtcggc ccagcgctcg ggggtggtgg ccacctcgag
     1981 gccgccgacc tggttgaaga cccagccgtc gcggtgttcc agcgagcaga acttgtcgat
     2041 ggtgtatccc gcgaaagcgc tcatcgtctt cgacggattg gtttggaaca ccagaccggg
     2101 cgcgtgggta gaggacccgc cggtggcgaa cagcggcccc cggtcgagca ccgtgaggtc
     2161 cgtgagtccg cgcccggtga gctcgtcggc gagggccgcc ccgacgaccc cggcaccgat
     2221 cacgacgact tttggcatgg cgagccgatc tgtcggctgt tgcttatagc gcaacgcgtt
     2281 gtgcaagacg caacaaatgg tcctccagcg aagtcaccac gtcaacagga acacgccgaa
     2341 aagaacaacc tggcaacggg ctgtgagcag gagacctgcg agtggagcgc ggctactctt
     2401 ctgagctggt cgagtcctcg ggcagcacgg cctcgagggc aggcccggtg atgcggcgga
     2461 atgcccgccg cgggcggttc gtgtcgagga tggcgacctc gagtgtgtcg gggccgagga
     2521 cacggtcctc cgagccgttg ccacccgcct tgagagccgc gacggcgatc ttcaccgcct
     2581 ccgagagccc ggtgttctcg gcataggagt ccttgagctt ctcgatgatc ggctccgtcg
     2641 tgccgcccat caccacgaag tgcggttcgt cggcgatcga gccgtcatag gtgatgcggt
     2701 agagctccgg cgccttggtc tcgccgaagt gtgcgacctc ggccacacac aactcgacct
     2761 catacggctt ggcctgctcg gtgaagatcg tgcccagcgt ctgggcgtac acattcgcca
     2821 gctgacggcc ggtgacgtcg cgtcggtcgt aggcgtagcc ccgcgtgtcc gcgaattgga
     2881 tgccgccgcg gcgcaggttg tcgaactcgt tgaaccgtcc gaccgctgcg aaccccactc
     2941 ggtcgtacag ttcgctgacc ttctgcagcg accgcgatgg gttttccgcg acgaacagca
     3001 caccggagtc gtacgcgagc accaccacgc tacggccccg ggagataccc ttgcgcgcga
     3061 gttccgaccg ttcgcgcatc gcctgctcgg gcgagatgaa atacggaaag ctcacgagtc
     3121 gccccgcgca tcggtcgacg gcccggacgt cgtgcggaat gtgtcttcgc gagaacgact
     3181 ttcgatgacc tcacgggcca acgccgcgat ccgctgctcg ggcacctcct cggcaccctc
     3241 ggcaccgagg gtcaccgccg tcgggtagat gccacgcacc agatcgggcc cgccggtcgc
     3301 ggagtcgtcg tcggccgcgt cgtagaggcc ctcgatggcc acccgcagtg ccgactcggc
     3361 atcgaccacc cgcgagtaca gcttcttgat cgacgacttg gcgaagatcg agccggaacc
     3421 caccgactgg tagccctctt cctcgaagtt ccagccgccc gcggcgtcga acgagacgat
     3481 gcgccccgcc gcctccggat cggggtcgtc gaggtcgtag ccggccagca acggcagcgc
     3541 gatgaagccc tgcagtgccg caccgagatt gccgcgcacc atggtcgcca accggttgac
     3601 cttgcccggg aacgtcagcg gcacaccttc gaccttctcg tagtgctcga gttcgacggc
     3661 gtagaggcgg gcgaattcga cggcgatcgc cgccgtgccc gcgatgccgg tcgccgtgta
     3721 gtcgtcggtg atgtacacct tcttcacgtc acggctggcg atcatgttgc cctgggtcgc
     3781 gcggcggtca cccgccatca ccacaccacc gggataggtc agcgcgacga tcgtggtgcc
     3841 gtgcggcagc gcatcacccg ctcctatgcc ctcggcaccg gttctgttca cgggtaggag
     3901 ttcgggcgcg gaacgacgaa gcaattcaga gaaggacgaa agatctacac gcacgtgagg
     3961 agcatagaag gcttgcggaa ctgacggagc tccgggaagg ggtgacggga agtgcagctg
     4021 atcgcgttgc gtccagctca ctggccaccc ttctggacgt atgcgcgaac gaagtcttcg
     4081 gcattctcct cgagaacgtc gtcgatctcg tcgagcagat cgtcggtctc ctcggtcagc
     4141 ttctcgcgac gctcttgccc ggcggccgtg ctgccggtgg ggtcgtcgtc ctcgccgcca
     4201 ccaccgccac gcttggtctg ctcctgagcc atcgctgcct cctgcatttg tcatcggcgc
     4261 ctatggccgc ccgccgttac ttcacactac cgttcggcag ccggattgcc cgggatagca
     4321 cggctaattg gtgagctgct ctaccagttc tacggcgctg tcaacggagt ccagcagggc
     4381 cccgacgtgc gctttactgc cccgcagcgg ctccagggtc ggaatccgga cgagcgaatc
     4441 accgccgaga tcgaagatca ccgagtccca gctcgccgcc gcgatgtcgg cgccgaagcg
     4501 tcgcaggcac tccccacgga agtaggcgcg cgtgtcggtc ggcggattgt cgacggcgtc
     4561 gcgcacctgc tgttcggtgg tcagccgctt catcgagccg cgcgccacca gccggttgta
     4621 gagccccttg tcgaggcgca catcggagta ctgcagatcg accaggtgca ggcgcggtgc
     4681 cgaccagttg aggttctccc ggttgcggaa gccttcgagc agacggagtt tcgcgggcca
     4741 gtcca
//
LOCUS       DASYLX010000294         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1548750,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000294 DASYLX010000000
VERSION     DASYLX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548750"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGP27_07165"
     CDS             <1..226
                     /locus_tag="VGP27_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HEV7851131.1"
                     /translation="HFDFRADDDNYFEQPGDLFRLMTGDEQQRLFDNTARAIKGARHQ
                     TVEMHIANCTQADAAYGEGVRKACEALGAL"
     gene            260..703
                     /locus_tag="VGP27_07170"
     CDS             260..703
                     /locus_tag="VGP27_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV7851132.1"
                     /translation="MVDNGIDDPRATLDIEVMDLAARLFDWARSGDAEMLAAYVDAGV
                     PVNLTNANGDTLVMLAAYHGHESAVAALIARGADVNRHNNRGQTPLAGAVFKNDTTII
                     ELLLSADADPLAGSPSALETARFFEREELARQLLQHIRRRRRVNV"
     gene            complement(1067..1252)
                     /locus_tag="VGP27_07175"
     CDS             complement(1067..1252)
                     /locus_tag="VGP27_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851133.1"
                     /translation="MPAAVAAFGTGRLLYGSDYCWTPAYAVDAQLASIDQDDQPADDS
                     WRALTTRNATRLLRPDR"
     gene            1751..1961
                     /locus_tag="VGP27_07180"
                     /pseudo
     CDS             1751..1961
                     /locus_tag="VGP27_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873641.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            1957..2343
                     /locus_tag="VGP27_07185"
                     /pseudo
     CDS             1957..2343
                     /locus_tag="VGP27_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304638.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2598..2807)
                     /locus_tag="VGP27_07190"
     CDS             complement(2598..2807)
                     /locus_tag="VGP27_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851134.1"
                     /translation="MKLQYVAPLAIAASAAAILLAPIAAADATVQQSPGNAQIIAIPG
                     AAAQEAAQNQQPSGGDSGALVFHHR"
     gene            complement(2903..3208)
                     /locus_tag="VGP27_07195"
     CDS             complement(2903..3208)
                     /locus_tag="VGP27_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851135.1"
                     /translation="MTTVSAGRSTRNRLRVHPAVVTAAAAMVAVAAVGVGAQFIAVTP
                     PATDTSLYCAGAFQCLQPDADRLLPDALGPLVPFGPAMSNSATPSRPVASPHSGGAV"
BASE COUNT          623 a         1029 c         1128 g          590 t
ORIGIN      
        1 tcacttcgac ttccgcgccg acgacgacaa ctacttcgag cagcccggtg acctgttccg
       61 gctcatgacc ggcgacgaac aacaaaggct cttcgacaac accgcacgcg ccatcaaggg
      121 cgcccggcac caaacggtcg agatgcacat cgcgaactgc acccaagccg acgccgccta
      181 cggcgaaggc gtgcgcaagg cgtgtgaagc cctcggcgcg ctctaacaac cagtcggccg
      241 cgaccagtgt ggagttgata tggtagacaa cgggattgac gacccgagag ccaccctcga
      301 tatcgaagtc atggatctgg cggcgcgctt gttcgattgg gctcgcagcg gcgacgccga
      361 aatgttggcc gcttatgtcg acgcgggcgt accggtgaac ctcaccaacg ccaacgggga
      421 caccctggtg atgctggcgg cctatcacgg acacgagtcc gctgtcgccg ctctgatcgc
      481 gcgtggggcc gacgtgaatc gacacaacaa tcgaggacaa actccgttgg caggcgccgt
      541 cttcaaaaac gacacaacga tcatcgaact gctgctgtca gccgatgccg atccgctggc
      601 aggctcgccg tccgcacttg agaccgcgcg gttctttgaa agggaggaac tcgcgcggca
      661 actgttgcaa cacatccggc ggcggcgcag agtgaacgtc tagctgcaac gcgcacactt
      721 ggcatcgggc agaccatttc gcgcagtgcg gggaatctag tgcagtctcg ctgggcgcac
      781 gaaatgcggc actccccttg aggttcaact aacgtaagtg cgtgctggaa accccgcaga
      841 cattctggct atgtatcacc gaaatcattt gacactaaat gagacggcgg tgttcgggcg
      901 ggctatccga tggcatgtca ggaaggtgcg cgatatcaat cccggtctgc cgtcacggtt
      961 ttccatccag ctaatgattg cgcggtggtc ttgggattca gacggatcac ttggcaaccc
     1021 gcggcggcag gggccgtgac gagggtcgcg gccgtttgac aggcagctac cggtcgggac
     1081 gcagcagccg ggtggcgttg cgggtggtga gtgcccgcca gctgtcgtcg gcaggctgat
     1141 cgtcttggtc gatggatgcc agctgggcgt ctactgcgta ggccggggtc cagcagtagt
     1201 cgctgccgta gagcaggcgc ccggtgccga acgccgcgac agcggcaggc acttggtgcg
     1261 gaaatggcgt gccgggcgat gtcgaaccac aaccgtctca gctggcgggg cacagcgcag
     1321 tccgggtcat cgtcgccgat aaacacagtg cggaagagct ccatctgatc ggcgagcagc
     1381 agtagagcgc cccaccatgg gtgaagaccc attcgatgtc ggggtagcgg acaagcgtgc
     1441 cgtgaagaca aggtcgctga ccgcgcgggt ggtgtcaaaa atgaactcca gcatcggccg
     1501 ggccggccga gggcgatcgc ttcgggaccg cttggtggcg gtggccgagt cgtggccaga
     1561 gccggcagat cgggggcgcc gaggtggtgt cgcggcacgc attgtgctca caccttcgta
     1621 ttgactgatt atcaatcagt ctgagatagc agggatcatt cgcatgcagg tcagtctccg
     1681 gcgattccgc cgattccggc gactcaacga aagcgctcag catgttcgca ccacaacggg
     1741 atgcccgggc atgacgggcc gggctcatcg ggtcacgctc gccggtgcgc gtgttctgct
     1801 caccgggggc ctccagcggt attggccggg ctttggcggt gcttctcgcc gcggaagggg
     1861 cccagcttgt ggtggccgcg cgtcgggcgg atctgattga ggcgctcggc gaggagatcg
     1921 ccgccgcagg ccatacgcag ccgtttgcgg tacctaccga ccatcctgtc gaagggcgga
     1981 ctcggtcccc gcgctcaaga cactggcccg ccgatcggcc atcccggtca ccctcgacct
     2041 cacgatcgac caacgcctac aggactcagt tgaagtcggc gcgtattacg tcgtgcccga
     2101 agcactgacc aacgccgcca ggcactctcg tgctacccag gtggtcatat ccggacacac
     2161 gaaggatcgc acgctctcgc tgtcgattca cgacgacgga atcgggggtg ccgacatggg
     2221 caacggctca gggctcctcg ggctcgcaga ccgggtcgag gcactcggcg ggcggatgcg
     2281 catcaagagc ccagccggaa ggggacgtcg cgggaggtca cgattccact cgacagcacg
     2341 tgagccctga atgtggcgcg cggcgacgga cgcctccggg tagctgggat acagcttcaa
     2401 ttggtgtcag cgcgcgcctc gcgcggcttt cagggctgaa ttcgattgtc tcgaaagggc
     2461 tctcgaaccg ggctgaccag tggagatcgt ttccagccgt ccccggtcaa ctctccgaat
     2521 tcgcacctag tgtgacaccg ccgtgacagg gtgagccacg gcttaccgga cgcgaggccc
     2581 taagcgcgcg gtcgttgtca gcggtggtgg aataccaggg cgcccgagtc cccgccggac
     2641 ggctgttggt tctgggcggc ctcttgcgcg gccgccccgg gaatagcgat gatttgagca
     2701 tttcccgggc tctgctgcac ggtagcgtcg gccgcggcga tcggtgccag tagaatggct
     2761 gccgcggatg cggcgatggc cagtggtgcg acgtattgca gtttcatttt ttctcctttt
     2821 tgggtgacat cgcctagaaa gcgatgtcgg ctgaaaatcg ggttccgccg acttactcgc
     2881 ggcagctgat gcaagcgtgg ggtcagacgg cgccgcccga gtgcgggctg gcgactggcc
     2941 gggacggcgt tgcggaattc gacatcgcag gaccgaatgg gacgagcggg ccaagggcat
     3001 ctgggagcag ccgatccgcg tcgggctgta ggcactggaa agccccggcg cagtaaagcg
     3061 aggtatcggt agccggcggc gtgacagcga tgaactgtgc accgacaccg accgcagcga
     3121 cagccaccat ggcagcggcc gcggttacta ccgccggatg cacccgcagt cggttgcgcg
     3181 tcgagcgacc tgcgctgacc gtagtcatcg tcttcctcca tatgcttttt cagcctcggt
     3241 gtatatccca agactgctcg acgcgggcct cgctgtcgtc ggcgccagcc accaaacccg
     3301 cttggggaga gccggaatcc cgcatggggg ttagtcggaa tgctgcagtt gagacggtga
     3361 cgtggcacct
//
LOCUS       DASYLX010000295         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2425950,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000295 DASYLX010000000
VERSION     DASYLX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425950"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VGP27_07200"
     CDS             <1..748
                     /locus_tag="VGP27_07200"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV7851136.1"
                     /translation="IVPVDSEHSAIAQCLRGGTPDEVAKIVLTASGGPFRGWTADRLE
                     DVTPEQAGAHPTWSMGPMNTLNSASLVNKGLELIETHLLFGIPYERIKVVVHPQSIVH
                     SMVTFTDGSTIAQASPPDMKLPIALALGWPSRVPAAAPACDFSSASTWEFEPLDTEVF
                     PAVELAREAGSRGGCLTAVYNAANEEAAAAFLDGRISFPAIVRTIADVLRAADQWAAE
                     PATVEEVLDAQRWARDRARGAVSREVVTTK"
     gene            745..1971
                     /locus_tag="VGP27_07205"
     CDS             745..1971
                     /locus_tag="VGP27_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEV7851137.1"
                     /translation="MMWVIGVVLFALGILISVALHECGHMWVARATGMKVRRYFVGFG
                     PTLWSTRRPNKLGYTEYGVKAIPLGGFCDIAGMTSIDEIAPEDRRYAMYKQKTWKRAA
                     VLFAGPGMNFVVGLVILYGIALHWGLPDLSPKPAAYVSQTTCVAPAISKDKMADCTGE
                     GPAAAAGIKQGDVVVKVGNTDVHNPDEMVAAVRAASGPTPFVVERDGKQITTVVDVVE
                     TKRYTSDTAQTPSTVGAVGVAAAELGPVKYTHYNPVTAIPGTVAFTGDLSVELGKSLA
                     KIPTKIGALVQSIGGGQRDPETPISVVGASRIGGDAFERGLYLAFWFFLAQLNFVLGA
                     VNLVPLLPFDGGHVAVAWYEKIRNVIRRARGMAAAAPVNYVKLMPATYVILVFVVGYM
                     ALTVTADFVNPIRPFE"
     gene            1981..>2582
                     /locus_tag="VGP27_07210"
     CDS             1981..>2582
                     /locus_tag="VGP27_07210"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV7851138.1"
                     /translation="MSVGLGMPAPPVPTLAPRRKTRQLMVGDVGIGSDHPIAVQSMCT
                     TKTHDVNTTLQQIAELTASGCDIVRVACPRQEDADALSEIAKHSRIPVIADIHFQPKY
                     IFAAIDAGCAAVRVNPGNIKEFDGRVAEVAKAAGAAGIPIRIGVNAGSLDKRFMEKYG
                     KATPEALVESALWEASLFEEHGFGDIKISVKHNDPVIMVAA"
BASE COUNT          443 a          852 c          868 g          419 t
ORIGIN      
        1 gatcgtgccg gtcgactccg agcattcggc gatcgcccag tgcctgaggg gcggaacccc
       61 cgacgaggtg gcgaaaatcg tgctgacggc ctccggcggg cccttccgcg gctggaccgc
      121 cgaccgcctc gaggacgtca cgcccgagca ggccggcgcg cacccgacct ggtcgatggg
      181 tccgatgaac acgctgaact cggcgtcgct ggtcaacaag ggcctggagc tgatcgagac
      241 ccacctgctg ttcggcattc cgtatgagcg catcaaggtg gtggttcacc cgcagtcgat
      301 cgtgcactcg atggtgacgt tcaccgacgg ttcgacgatc gcccaggcca gcccgccgga
      361 catgaagctg ccgatcgcgc tggcgctggg ctggccgtcg cgggtgcccg ccgccgcgcc
      421 ggcgtgcgac ttcagctccg cttcgacctg ggagttcgag ccgctggaca cggaggtctt
      481 tccggccgtc gagctggcca gggaggcagg cagccggggc ggctgcctga ccgcggtcta
      541 caacgcggcg aacgaagagg ccgctgcggc gttcctcgac ggccggatca gtttcccggc
      601 catcgtgcgc accatcgccg acgtgctgcg tgctgcagac cagtgggccg ccgaacccgc
      661 taccgtggaa gaagtgcttg acgcgcaacg ctgggcccgg gacagggcaa ggggcgcggt
      721 ttctcgggag gttgtcacca caaaatgatg tgggtcatcg gcgtagtact tttcgcgctg
      781 ggcatcttga tatcggtggc gctgcacgag tgcgggcaca tgtgggtggc gcgtgccacc
      841 gggatgaagg tgcgccgtta cttcgtcggg ttcggtccga cgctgtggtc cacccgacgg
      901 cccaacaagc tgggctacac cgaatacggc gtcaaggcga tcccgctcgg tgggttctgc
      961 gacatcgccg ggatgacgtc gatcgacgag atcgccccgg aagaccgtcg gtatgcgatg
     1021 tacaagcaga agacgtggaa acgcgccgcc gtgctgttcg ccggcccagg catgaatttc
     1081 gtcgtcggcc tggtgattct ctacggcatt gcgctgcatt ggggcctacc cgacctgagc
     1141 ccgaagcccg cggcctatgt cagccagacc acttgtgtcg caccggcgat cagcaaggac
     1201 aagatggccg actgcacggg tgaggggcct gctgccgccg cgggcatcaa gcagggcgac
     1261 gtcgtcgtca aggtgggcaa caccgacgtg cacaatcccg acgagatggt cgccgcagtc
     1321 cgcgcggcca gcggcccgac accgttcgtc gtcgagcgcg acggaaagca gatcaccacg
     1381 gtcgtcgacg tcgtcgagac gaagcgctac acgtccgaca ccgctcaaac tccgtcgacc
     1441 gtcggcgcgg tgggcgtcgc cgccgctgag ctcgggcccg taaagtacac gcactacaac
     1501 ccggtcaccg caattcccgg cacggtggcg ttcaccggcg atctgtccgt cgaactgggc
     1561 aagtcgctgg cgaagatccc gaccaagatc ggggccctgg tgcagtccat cggtggcggc
     1621 cagcgcgatc cggaaactcc gatcagcgtc gtgggcgctt cccgtatcgg cggcgacgcc
     1681 ttcgaacgag ggctttacct cgcgttctgg ttcttcctcg cgcaactgaa cttcgtgctc
     1741 ggcgcggtaa acctggtgcc cctgctgccg ttcgacggcg gccacgtcgc ggtggcgtgg
     1801 tacgagaaga tccgcaatgt gatccgcagg gcccggggca tggccgccgc ggcgccggtg
     1861 aactacgtca agttgatgcc cgccacttac gtgatcttgg tgttcgtggt cggttacatg
     1921 gcgctgaccg tgacggccga cttcgtcaac cccatcagac ccttcgagta ggagacctcc
     1981 atgtccgtcg gcttagggat gccggcacct ccggtgccca cactggctcc gcggcgaaaa
     2041 acccggcagc tcatggtcgg cgacgtcggc atcggcagcg accacccgat cgcggtgcag
     2101 tcgatgtgca ccacgaagac ccacgatgtg aacaccacgc tgcagcagat cgcggagctc
     2161 accgcgtccg gctgcgacat cgtccgggta gcctgcccgc gacaggaaga cgccgacgcg
     2221 ctttcggaga tcgccaagca cagccggatt cccgtcatcg ccgacatcca cttccagccg
     2281 aagtacattt tcgccgcgat cgacgcaggc tgcgccgccg tgcgcgtcaa ccccggcaac
     2341 atcaaggagt tcgacgggcg ggtcgcggag gtcgcgaagg cggcaggcgc agcggggatt
     2401 ccgatccgca tcggcgtcaa cgcgggctcg ctggacaagc gcttcatgga gaagtacggc
     2461 aaggccacgc cggaggccct cgtcgagtcc gcgctgtggg aggcgtcgct gttcgaggag
     2521 cacggcttcg gcgatatcaa gatcagcgtc aagcacaacg acccggtcat catggtcgcc
     2581 gc
//
LOCUS       DASYLX010000296         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3522665,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000296 DASYLX010000000
VERSION     DASYLX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522665"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGP27_07215"
     CDS             complement(<1..327)
                     /locus_tag="VGP27_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7851139.1"
                     /translation="MHTELCDRFGITYPIFVFTPSEKVAAAVSKAGGLGVLGCVRFND
                     PDDLEGALHWMDENTDGSPYGVDVVMPAKIPAEGTSADIGKLIPQAHRDFVGKTLADL
                     GVPPLPE"
     gene            complement(414..2072)
                     /locus_tag="VGP27_07220"
     CDS             complement(414..2072)
                     /locus_tag="VGP27_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7851140.1"
                     /translation="MSVALNIADLAEHAIDAVPDRVALICGDDQLTYAQLEEKANRLA
                     HYLIDQGVKKDDKVGLYCRNRIEIVIAMLGIVKAGAILVNVNFRYVEGELKYLFENSD
                     MVALVHERRYADRVANVLPETPNVKTILAVEDGSDDDFQRYGGVEFFAALEQASPERD
                     FGERSADDIYLLYTGGTTGFPKGVMWRHEDVYRVLFGGTDFATGEFISDEYDLAKAAA
                     ENPPMIRYPIPPMIHGATQSATWMSVFSGQTTVLAPEFDADEVWQTIEKHKVNLLFFT
                     GDAMARPLLDALTKMHDSGHEPDLSSLFLLASTAALFSTSIKEKFLELLPNRVITDSI
                     GSSETGFGGTSIVAKGQSHTGGPRVTIDQRTVVLDEDGNEVKPGSGVRGLIAKKGNIP
                     VGYYKDEKKTAETFKTFNGVRYAIPGDYAEVDADGSVTMLGRGSQSINSGGEKIYPEE
                     VEAALKGHPDVFDALVVGVPDERFGNCVAAVVHRRPGTNPTLAELDTFVRHEIAGYKV
                     PRKVWWVDEIHRTPAGKPDYRWAKDTTEERPADDVHANHVGTPS"
     gene            2138..>2844
                     /locus_tag="VGP27_07225"
     CDS             2138..>2844
                     /locus_tag="VGP27_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7851141.1"
                     /translation="MSDGENLEKGPDALIEQRGHTLILTLNRPEARNALSTEMLSIMV
                     EAWDRVDADPEIRSCILTGAGGYFCAGMDLKAATAKPPGDSFADGSYDPSRIDALLKG
                     RRLTKPLIAAVEGPAIAGGTEILQGTDIRVAAESAKFGISEAKWSLYPMGGSAVRLAR
                     QIPYTVACDLLLTGRHITAAEAKEMGLVGYVVPDGTALEKALEIAEVINKNGPLAVQA
                     ILKTIHETEGMHENDAF"
BASE COUNT          478 a          915 c          928 g          523 t
ORIGIN      
        1 ctcgggcagc ggcggcacac cgaggtcggc gagcgtcttg ccgacgaagt cgcgatgcgc
       61 ctgcgggatc agcttgccga tatcggcgga cgtgccctcg gccggaatct tggcgggcat
      121 cacgacgtcg acaccgtagg ggctgccgtc ggtgttctcg tccatccagt gcaacgcgcc
      181 ttcgagatcg tcggggtcgt tgaaccggac gcatccgagc acgccaaggc cgcccgcctt
      241 gctgactgcg gccgccacct tctccgacgg ggtgaagacg aagatcggat aggtgatgcc
      301 gaagcggtca cacagttcgg tgtgcatcta cttcccccgt cgcttcgctc gccccatcta
      361 cttgccccgt ctcgctcgcc ccatctactt cccccgtcgc ttccctcgcc ccatcaggac
      421 ggcgttccga catggttggc gtgcacgtcg tcggcaggac gctcctcggt ggtgtccttg
      481 gcccaccggt agtcgggctt gcccgcagga gtgcggtgga tctcgtcgac ccaccaaacc
      541 ttgcgcggca ctttgtaacc ggcgatctcg tggcgcacga acgtgtcgag ttcggccagt
      601 gtcgggttgg tcccgggcct gcggtgcacg acggcggcaa cgcagttgcc gaatcgctcg
      661 tcgggcacgc cgaccaccag cgcgtcgaac acgtcgggat ggcccttgag tgcggcctcg
      721 acttcctcgg ggtagatctt ctcgccaccg ctgttgatcg actgcgaccc gcggccgagc
      781 atcgtgacgc tgccgtccgc gtcgacctcg gcgtagtcac cggggatcgc atagcgaacg
      841 ccgttgaagg tcttgaaggt ctccgcggtc ttcttttcat ccttgtaata gccgaccggg
      901 atgttgccct tcttcgcgat cagcccgcgg acgccggagc cgggcttgac ctcgttgccg
      961 tcctcgtcga gaaccactgt gcgctggtcg atcgtcaccc gcgggccgcc ggtatgcgac
     1021 tggcccttgg ccacgatgct ggtgccgccg aagcccgttt cggaggaccc gatcgagtcg
     1081 gtgatcaccc ggttgggcag cagctcaagg aatttctcct tgatgctggt cgagaacaag
     1141 gccgccgtgc tcgccaacag gaacaggctc gacaggtcag gctcgtgacc ggagtcatgc
     1201 atcttggtca acgcgtcgag cagcgggcgg gccatcgcat cgccggtgaa gaacagcagg
     1261 ttcaccttgt gcttctcgat cgtttgccac acttcgtcgg cgtcgaattc cggtgccagc
     1321 actgtcgttt gacccgagaa caccgacatc caggtagccg actgcgtcgc accgtggatc
     1381 atcggcggga tcgggtagcg gatcatcggc ggattctccg cggcggcctt ggcaaggtcg
     1441 tactcgtcgg agatgaactc accggtcgcg aaatcggtgc cgccgaacag cactcggtag
     1501 acgtcctcgt gacgccacat gacgcccttc ggaaaaccgg tggtgccgcc ggtgtagagc
     1561 agatagatgt cgtcggcgct gcgctcgccg aaatcgcgct caggcgaggc ctgctcgagc
     1621 gccgcgaaga actcgacgcc gccgtagcgc tggaagtcgt cgtcgctgcc gtcctcgact
     1681 gcgaggatgg tcttgacgtt gggcgtttcg gggagcacat tggcgacccg gtcggcgtag
     1741 cgccgctcgt gcaccagcgc aaccatgtcc gagttctcga aaagatactt cagctcgccc
     1801 tcgacgtagc ggaagttaac attgaccagg atggcgccgg ccttcacgat gccgagcatc
     1861 gcgatgacga tctcgatgcg gttgcggcag tacaggccga ccttgtcgtc cttcttcaca
     1921 ccctggtcga tcaggtagtg cgcgaggcgg ttggccttct cctccagctg cgcgtaggtc
     1981 agctggtcgt cgccgcagat cagggcgacg cggtctggca cggcgtcgat ggcgtgctcg
     2041 gcgaggtctg caatgttcag ggccacggac accaaactag aacgtgttac atttcgtgac
     2101 aagtctgtgg catcgatgca ggaaggcgga cggttccgtg agcgacggtg aaaatctcga
     2161 gaaaggcccc gacgccctca ttgagcagcg cggacacacg ctgatcttga cgctcaaccg
     2221 ccccgaggcg cgtaacgcac tttccaccga gatgctctcg atcatggtcg aggcgtggga
     2281 ccgcgtcgat gctgatcctg agatcaggtc gtgcatcctg accggtgccg gcggctactt
     2341 ctgcgccggg atggacctca aggcggcgac ggccaagccg ccgggcgact cgttcgccga
     2401 cggcagctac gacccgtcgc gcatcgacgc gctgctgaag gggcgccggc tgaccaagcc
     2461 gctcatcgca gccgttgagg ggcccgcgat agcgggcggc accgagatcc tgcagggcac
     2521 cgacatccgg gtcgcggccg aaagcgccaa gttcggcatc tccgaggcca agtggagcct
     2581 gtatccgatg ggcggttccg ctgtgcggct ggcgcgccag attccctata ccgttgcgtg
     2641 cgacctgttg ctgaccggac ggcacatcac cgcggccgag gccaaggaga tgggccttgt
     2701 cggatacgtg gtgccggacg gcacagcgct ggagaaggcg ctcgagatcg ccgaggtgat
     2761 caacaagaac gggccgctcg cggtgcaggc cattttgaag acgatccacg agaccgaggg
     2821 catgcacgag aacgacgcct tcaa
//
LOCUS       DASYLX010000297         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2316787,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000297 DASYLX010000000
VERSION     DASYLX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316787"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VGP27_07230"
     CDS             complement(<1..122)
                     /locus_tag="VGP27_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007411535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HEV7851142.1"
                     /translation="MCRNITELRGLEPAATAEEIEAAARQYIRKVSGVTRPTAA"
     gene            145..903
                     /locus_tag="VGP27_07235"
     CDS             145..903
                     /locus_tag="VGP27_07235"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7851143.1"
                     /translation="MTSDDVLLIDTTDRVRTLTLNRPRSRNALSSALRRQFFAALRDA
                     EADDSVDVVVLTGADPVFCAGLDLKELGDTTELPDISPKWPPMTKPVIGAINGAAVTG
                     GLELALYCDVLIASEQARFADTHARVGLLPTWGLSVRLPQKVGAGMARRMSLTGDYLS
                     AGEALRAGLVTEVVPHDQLMPTAWRTAAAIVGNNQKAVRALLASYHRIDDAQTNEGLW
                     IEAASARQWMSTATGHDIAANRAAVIQRGRTQVR"
     gene            924..1490
                     /locus_tag="VGP27_07240"
     CDS             924..1490
                     /locus_tag="VGP27_07240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851144.1"
                     /translation="MNKAHEMCGSDEWRKAMREVILPWALNGTDLGDEVLEVGPGYGA
                     TTDVLSHAVPRLTSVEIDRELAAMLADRFADVPSVEIVNADATSLSYDDNRFSGAACF
                     TMLHHVPTSELQDRLFAEVARVLRPGAALVASDSLGSEELKAHHEDDTYNPVDPATLC
                     DRLSAAGFTDVDVRTNDYGWAVIARIAG"
BASE COUNT          269 a          568 c          547 g          251 t
ORIGIN      
        1 ttggctgccg tcggccgcgt cacgccgctc accttgcgga tgtactgccg cgcggcggcc
       61 tcgatctcct cggcggtcgc ggccggttcc aggccgcgga gctcggtgat gtttcggcac
      121 attcgtccag gataggttca ggccatgact tccgacgatg tgctgctgat cgacaccacg
      181 gaccgggtcc gcaccctgac gctgaaccgc ccgcgatcgc gcaacgcgct gtcgtctgcg
      241 ctgcgtagac agttcttcgc cgccctgcgc gatgccgagg ccgacgactc cgtcgacgtg
      301 gtcgtcctga ccggcgccga cccggtgttc tgtgccgggc tcgacctcaa ggagctcgga
      361 gacaccaccg agctgcccga catctcgccg aagtggccgc cgatgacgaa gccggtgatc
      421 ggagccatca acggcgcagc cgtcaccggc gggctcgagc tcgcgctgta ctgcgacgtc
      481 ctgatcgcct cagaacaggc ccggttcgcc gacacccacg ccagggtggg actgctgccg
      541 acttgggggc tgtcggtgcg actaccccag aaggttggcg cgggcatggc ccgccggatg
      601 agcctgactg gtgactatct ctcggccggg gaagcgttac gggccggtct ggtcaccgag
      661 gtggtgccgc acgaccagct gatgccgacg gcgtggcgga cggccgcagc gatcgtcggc
      721 aacaaccaga aggcggtccg cgcgctgctg gcgtcctacc accgcatcga tgacgcacag
      781 accaacgaag gcctgtggat cgaggcggcg tcggcgcggc aatggatgag caccgccacc
      841 ggccacgaca tcgccgccaa ccgcgcagcc gtaatccaga ggggccgcac acaagtgcgc
      901 tgaacgcgcc taggctgccg atcatgaaca aagcgcacga gatgtgcggc agcgacgagt
      961 ggcgaaaggc gatgcgcgag gtcatcctgc cgtgggcgct gaacggcacc gacctcggcg
     1021 acgaggtgct cgaggtcggg cccggctacg gcgccaccac cgacgtgctc agccacgcgg
     1081 tgccgcgact cacctcggtg gagatcgacc gcgagctggc tgcgatgctg gccgaccgct
     1141 tcgccgacgt cccttccgtc gagatcgtca acgcggacgc gacctcgctg tcctacgacg
     1201 acaaccggtt ctccggcgcc gcgtgtttca ccatgctgca ccacgttccg acgagcgaac
     1261 tgcaggaccg gctgttcgcc gaggtcgctc gcgtgctgcg acccggcgcc gcgctcgtcg
     1321 ccagcgacag cctcggcagc gaggaactca aggcccacca cgaagacgac acctacaacc
     1381 ccgtcgaccc ggcgacgctg tgcgaccggt tgtcggcagc cggcttcacc gacgtggacg
     1441 tgcgcaccaa cgactacggc tgggccgtca tcgcgcgcat cgccggctga ctcaaggctg
     1501 gggttcgaac tccatatcga catgaactac ctgttcgatg cccggctggt cggtgacggg
     1561 ttcggctgcg ttcggtccga aggtcgggct caaatcgccc tcgggactgg cgaactggac
     1621 gttgaagctg ggcat
//
LOCUS       DASYLX010000298         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2097743,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000298 DASYLX010000000
VERSION     DASYLX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097743"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VGP27_07245"
     CDS             <1..428
                     /locus_tag="VGP27_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7851145.1"
                     /translation="RFAVAMRDGDAVELSAVSEEFERMGDLVAAIDAAAHAARQYRQQ
                     GKRGSALGCSTRADALAAQCGAITPALRQATEDLPLTDRETEIVMLIGEGLSNRAIAE
                     RLTLSVRTVESYVYRSMVKTETTSRDELAALLPRRRAAK"
     gene            536..1585
                     /locus_tag="VGP27_07250"
     CDS             536..1585
                     /locus_tag="VGP27_07250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEV7851146.1"
                     /translation="MADHSRAGRHERSTEPVTIATVSSCMLAPKQVWGLADGAGDTVF
                     VFPGAGWHWTRVAVELLSDAPTFVDEMRRCDAAFTEFLDWSPLEFLRGDIGASGTDRV
                     DVMQPVLFAVMVSVAALARSRGIVPDAVLGHSQGEIAAAYVAGALSLRDAAKVVTSLS
                     EAISTISGAGGMVSIPLPIHHVCALIEPWGESLSVAAQNGPASTVVTGYAAALDELVA
                     ECERDRVPAVRIPVAYAAHSTQVEAMRETLRETLSDLQPRTGDIPFISGVTGAGLDTS
                     ILDGDYWFANLRQPVLFEQAVRWSYEHGYRTFLEVSPLPVLTWGIRESVDEYGAHHPV
                     VTATAPLAATPERTR"
     gene            complement(1775..>3415)
                     /locus_tag="VGP27_07255"
     CDS             complement(1775..>3415)
                     /locus_tag="VGP27_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEV7851147.1"
                     /translation="QAAIEARIDRLTAPAKRTLNAASVVGAHFGADLLAALGLDPVFD
                     ELLSVELIDQVRFTPGAEYAFRHPLIRAVAYESQLKSDRAQWHRRLATAIQERGPGSV
                     EDNAALVAEHLEAAGELRAAYGWHMRAAAWSTNRDLDAARLSWERARLIADRLPDDPD
                     RLSMRIAPRTMLCATDWLGRAADESRGRFAELQELCSAAGDNVSLAIGMSGLAAELLY
                     TGSSQGSRLASEQMALLESIGDPTLTVGLAFVAFANWFSVGEFGEILRWSQTIIDLAG
                     GDPAMGAGFGMGSPLAIALTFRGVARWWLGRPAWQQDLQDAIAMARNSDPATLGLVVT
                     WTYGLAIQYGVLRADDSALHAIEEAVETAQRASGDIALIFAEYTLGAALLGRDTAADR
                     PRGLELTVQARGWQRERMPSLVPVTELLVAQERARRGDRDAAIAVMRQAVDDLHQAGR
                     LGYGVWGTGILAESLLNRGTDGDLAEAQQAIDRLASLSDDGSAMREITLLRLRALLAR
                     ACGDEVTYRDLVSRYRAMAESFGFEGHIDWAESMAAET"
BASE COUNT          566 a         1144 c         1133 g          572 t
ORIGIN      
        1 cacgattcgc cgtcgccatg cgggacggtg acgccgttga actgtcggcg gtgtccgaag
       61 aattcgagcg catgggtgat ctcgtcgcgg ccatcgatgc cgcagcgcac gccgcacgcc
      121 aatatcgcca gcagggcaag cggggatcgg cgctgggatg ctcgacgcgt gcggatgccc
      181 tcgcggcgca atgcggcgca attacgcccg cgcttcgtca ggccaccgaa gacctgccgc
      241 tgaccgaccg cgaaaccgag atcgtgatgc tgatcgggga gggattgtcg aaccgggcca
      301 tcgccgagcg gctgacactg tcggtgcgca cggtcgagag ctacgtctac cggtccatgg
      361 tgaaaacgga gacaaccagc cgcgacgaac tcgccgccct gttgcctcgc cgcagggcgg
      421 cgaagtagtc caaggcggca cattgcgcga ttgcagtgct gcgttactgc gctcatgccc
      481 gcgacggcga gtgacgatct gattttgcga gcggcccgag cggaacggag catgtatggc
      541 cgaccacagc cgtgccggca ggcatgagcg cagcacggag ccggtcacca ttgcgaccgt
      601 ttcgtcctgc atgctcgcgc cgaaacaggt ctggggcttg gccgacggtg caggcgacac
      661 cgtcttcgta tttcccgggg cgggatggca ctggacgcga gtcgcggtcg aattgttgag
      721 cgacgcaccg acattcgttg acgaaatgcg ccgctgcgat gcggctttca ccgaattcct
      781 cgactggtcg ccgctcgagt tcctgcgggg ggatatcggt gcctccggta ccgaccgggt
      841 cgatgtcatg caacccgtgc tgttcgcggt gatggtctcg gtggccgcac ttgcgcggtc
      901 acggggaatc gttcctgacg ccgttctcgg ccattcgcag ggcgagatcg cggcggccta
      961 tgtcgccggg gcgctctctt tgcgagacgc agcgaaggtc gtcacgtcgc tcagtgaggc
     1021 gatcagcacg atttccggtg caggcggtat ggtctcgatc ccgttgccga tccaccatgt
     1081 ttgcgcgctg atcgaaccct ggggtgaatc tctttccgtc gccgcgcaga acggtccggc
     1141 ctccaccgtg gtcaccggtt atgcggcggc gctggacgaa ttggtggccg agtgcgagcg
     1201 ggaccgcgtg cctgccgtgc ggattcccgt cgcgtacgcc gcgcattcga ctcaggtcga
     1261 agctatgcgg gagacacttc gcgagacgct ttctgatctg caaccgcgaa ccggcgacat
     1321 tcctttcatc tccggggtca ccggcgccgg gctcgacacg tcgatcctgg acggcgacta
     1381 ctggttcgcc aatctgcgcc aacccgtgct cttcgaacag gccgtccgtt ggtcgtatga
     1441 gcacggttat cgcacatttc tcgaggtcag tccgctcccg gtgctgactt gggggattcg
     1501 ggaatctgtg gacgaatacg gcgcacacca cccagtggtc acagctacag cgccgctggc
     1561 ggcgacaccg gaaagaaccc gctaaaagca gtggtgcact gctgcactcg ccacccggca
     1621 tgaggatgac catggattgg ccaggacatc gcgaaagtta cgacgacaac gaaacagacg
     1681 ggggagcggg cgtagcgccg tgcgcacgcc cagtcggaac agcccgggcc ggggggtccc
     1741 gggttgttct tcgatgcagc ggccggcgcg ccgctcaggt ctccgccgcc atcgactcgg
     1801 cccagtcgat gtggccttcg aagccgaagg attccgccat cgcgcgatac cgactcacca
     1861 agtcccggta agtcacttcg tcgccgcagg cccgagccag cagcgcgcgc agtcgcagca
     1921 gcgtgatctc acgcatcgcc gaaccgtcat ccgacaggct cgccagccgg tcgattgcct
     1981 gttgggcttc agccagatcg ccgtcggtgc cacggttcag cagcgactcc gccagaattc
     2041 cggtgcccca aacgccatac ccgagccttc ctgcctggtg caggtcgtcg acggcttggc
     2101 gcatcaccgc aatggcagca tcgcggtcac cgcgcctggc cctctcctgg gcgaccaaca
     2161 gctcggtgac cgggaccagg gagggcatac gctcgcgctg ccacccgcgg gcctgcaccg
     2221 tcagctccag cccccggggg cgatcggccg cggtatcccg acccagtagc gcggcgccca
     2281 gcgtgtactc ggcgaagatc agtgcgatat ctccgctggc cctttgggcg gtctccaccg
     2341 cctcctcgat cgcgtgcagc gcggagtcat cggcacgaag cacgccgtac tgaatcgcaa
     2401 gaccgtaggt ccaggtgacg accaggccga gggttgccgg gtcgctgttt cgggccatgg
     2461 cgatggcgtc ctgaaggtcc tgttgccacg cgggacggcc cagccaccac cgagctacgc
     2521 cgcgaaacgt caacgcgatt gccagcggcg atcccatccc gaagccggcg cccatagcgg
     2581 gatcgccgcc ggccaggtcg atgatggtct gcgaccaccg cagtatctcg ccgaattcac
     2641 cgacactgaa ccagttggcg aacgcgacga acgccaagcc gacggtcaag gtggggtcgc
     2701 cgatcgactc gagcagcgcc atctgttcgg atgccagccg cgagccctgc gaactccccg
     2761 tgtacaggag ctcggcggcc agcccgctca tgccgatcgc cagcgagaca ttgtccccgg
     2821 cggcactgca caactcctgc agctctgcga agcgcccccg gctctcgtcg gctgctcggc
     2881 ccagccagtc ggtggcgcac agcatggtgc ggggggcgat gcgcatcgac aatcggtcgg
     2941 ggtcgtcggg tagccggtcg gcgatcaggc gtgcccgctc ccagctgagc cgggcggcgt
     3001 cgaggtcgcg gttggttgac cacgcggccg cgcgcatgtg ccagccgtac gccgcgcgca
     3061 gttcgcccgc tgcttcgagg tgttcggcga caagcgcggc gttgtcctcc accgagccgg
     3121 gcccacgctc ttgaatggcg gtggccaggc gccggtgcca ctgagcgcga tcggatttca
     3181 gctgtgattc gtaggccacc gcgcgaatca gtggatgccg gaaggcgtac tcggcgcccg
     3241 gtgtgaaccg cacctggtcg atcagttcga cactgagcag ctcgtcgaac accggatcga
     3301 gccccagtgc cgcaaggagg tccgccccga agtgggctcc aaccaccgac gccgcgttca
     3361 acgtccgctt ggcaggagcg gtcagccggt cgatgcgtgc ctcgatggct gcctg
//
LOCUS       DASYLX010000299         6985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3743334,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000299 DASYLX010000000
VERSION     DASYLX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743334"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VGP27_07260"
     CDS             <1..145
                     /locus_tag="VGP27_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV7851148.1"
                     /translation="NADAGFANRGTFVVDRSGIIRFAEMKQPGEARDQAVWTDALKAL
                     ESA"
     gene            190..264
                     /locus_tag="VGP27_07265"
     tRNA            190..264
                     /locus_tag="VGP27_07265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Val,seq:tac)
     gene            complement(751..1158)
                     /locus_tag="VGP27_07270"
     CDS             complement(751..1158)
                     /locus_tag="VGP27_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851149.1"
                     /translation="MNLTWGVTAAVVLAGSAVGFACPACADDLDGAYTTMWSDGKAPA
                     TWTFTPCGPGCRHVTSSKGWSTDAQLVNGSWIMGPVQNIVHCWSGTIETATENSSFDA
                     ATLSGTSVVTYPVACPQDPPGGLTIQFSLTKGK"
     gene            1949..2662
                     /locus_tag="VGP27_07275"
     CDS             1949..2662
                     /locus_tag="VGP27_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851150.1"
                     /translation="MPSQVSSRSSNQRETVARSLLDVLPGAFEALDYQAPFLIEKLVK
                     DRFVDTPDEACALFTEVKRYLVLTQVDQTKNWKMHSLCVDEAWHQFVLFTAEYSGFCT
                     KYFGCYRHHFPSNAPNAGLMYIPEATLAEFGDRYRDIFGTDLPQVWDDSSWVTPHRRV
                     VNRDCGQVALDSVDRMVALIDHDGRPLLSVSENAREALRFLVSAGAFYVRELPGDLTD
                     EEKVALVAALVKLKVLRVG"
     gene            2761..3798
                     /locus_tag="VGP27_07280"
     CDS             2761..3798
                     /locus_tag="VGP27_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851151.1"
                     /translation="MKLDDLCELFDRQEFPQSHVREHPDRDAVLAARSAVTAAVRDDE
                     FLVDCVAYELTRLKQPGLRPGLVPFFILPEFGVRFALGYWPPGRSAGAHEHTAWTITG
                     VCHNELTVQTYDRDQSYRHQTLVPKNRFDALAGQVGFIYEPCIHDPRNPTDRWSLSLH
                     VSSPRDGEQLTDQEQCLPILDEYFTRQRTDYGAPYEEVVATRRRQLKIRAIAGFLAQV
                     DAVPVADLLERCVQQGSSATSRFIHGLGRTDLMNGGRPTARTLARCHESLVLSYRETD
                     DFVALGVQTQRGWVEELAVSRVAREAIAFCVGAPRFDVLELPGCLTDDERWAIADALE
                     ESGLFTVAASE"
     gene            3791..4570
                     /locus_tag="VGP27_07285"
     CDS             3791..4570
                     /locus_tag="VGP27_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7851152.1"
                     /translation="MNDINAEAVGVVQGQSHTHRLLGTSAAMAALLIIAVTVAGCEGP
                     HPIASTQTTSPQRATTSLAPLVASDQLDSILLTVADVNAAMGASGMHPTTPIAHAPRE
                     VVERTLSNPDCLGAFFVGEPPAYQGSGYTAASIVTLHEMVNNPDHRLGQAVVSFPSAD
                     RALAFLKTSVGEWRACAGKTLVATHLEQVYRWTVGNLVGDAPAITQLNTLEGGNGYVC
                     QHVLRAVLNVVLDVNACGYHISDQAGRIAEKMAGSATRQAH"
     gene            5054..6049
                     /locus_tag="VGP27_07290"
     CDS             5054..6049
                     /locus_tag="VGP27_07290"
                     /inference="COORDINATES: protein motif:HMM:NF044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851153.1"
                     /translation="MTSRTSVDVLVVGTSIDPHIEAVTSLLPGQLVVARLDVDQYPCD
                     RALTISLSSCHRHIDLEQGQDRVDLTSAKVVWFRRFGPPVLATDVPSEYRRFCLAEAE
                     HLLDGVISLLRPSVWINEYWSTRRASTKPYQYSVAADVGFVTPQTLITNSASSAAEWI
                     ANRSEIVAKTLSTPIITDDDDERGFALTHVLSDSDKSDLQPVSVTATQFQDLIDVGYE
                     LRITSIGDKNFAVRIDSEAAEVQGVRDWRSEKIKSRYSWCELPSDVDEMLTRLHRALG
                     LSFAASDLIVDEHGEHYFLETNPHGRWRWLEKALGSSPITEYFAEYITKGTMAGI"
     gene            complement(6349..>6985)
                     /locus_tag="VGP27_07295"
     CDS             complement(6349..>6985)
                     /locus_tag="VGP27_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV7851154.1"
                     /translation="RRAALLKWAHDNNGLVIEDDYDAEFRYDRVSLGALQGLAPERVV
                     YAGCISKTLSPALRLGWMILPRWLVDGVAHQKLLDDMGTTVAEQLSLARFIETGGFTR
                     HLRRVRPIYRRRRDTMLEAVATSLPAAVPKGIAAGLHLYVQLPDWCDEARLLDAARTR
                     GLLIEGASWHWSEQQSAPPALILGYGAISEATILNALRRLGSIYRTQRRRA"
BASE COUNT         1387 a         2119 c         2109 g         1370 t
ORIGIN      
        1 caacgcggac gcgggcttcg ccaaccgcgg cacgttcgtc gtcgaccgct ccggaatcat
       61 ccggttcgcc gagatgaaac agcccggcga ggcgcgcgat caggcggtat ggaccgatgc
      121 gctgaaggcc ctggagtcgg cttaggaatt tccggtcgcc ggtcgttggc gtgtagcctg
      181 caccggcacg ggcgcgtagc tcagtggtag agctctggtt ttacacacca gcggtcggcg
      241 gttcgatacc gtccgcgccc accacaaagt cccgccgaaa agcacgtcgc ctgcggagac
      301 cagcgcgtcg tcgtagacat ggccgtacac gcgcaacgtc atcgccggat catgaccgag
      361 ccacttcgcc gttgcagacg ccgtcgtgcc gctgtcgagc atcttgtggc ggcggtgtgc
      421 ctggtgtcgt gcaaccggat gacgggcaca ccgaaccctc ttcgaatcgt ccggagattc
      481 cggtcaccgt ctcattggcc gaccgggtgc gccgggagaa ggcggcagtg gttgcacggc
      541 caagagtcgc gcccccgccg cgtaaccgcc gccagaaacc gagatgaagg cgggggtgga
      601 tgtcctcgag ccaggcgaag gaacgccaag tcgatccacc gcaatgcaag caattcagat
      661 caagaagtct ccggactcac cggagcggtt caggggcaac tgtagccagc ggaaccgctt
      721 cgcgttggcc ctggccaggt tcatccccac tcatttcccc ttcgtcaaac tgaactgaat
      781 ggttaatcca ccgggagggt cttgggggca tgcaactggg tatgtgacga cgcttgtgcc
      841 ggacagcgtc gccgcatcga aggagctgtt ctccgtggcc gtttcgatgg tgccgctcca
      901 gcaatgaaca atgttctgta ccgggcccat gatccaactg ccgttcacaa gttgcgcgtc
      961 tgtgctccag cctttgctgc tggtcacgtg gcggcaacct ggtccgcagg gggtgaaggt
     1021 ccaggtggca ggtgctttgc catcgctcca catggtggtg tacgcgccat cgagatcgtc
     1081 ggcacacgcc ggacaggcga aaccgacggc ggagcccgca agaactactg ccgcggtgac
     1141 accccatgtg aggttcatga tggtcctata ctggagtcag acctttgctc ccgcatgcga
     1201 tctgacaaac ggtcgccaac ggaaccgtaa gatctctacc gctcgtcgta tccgcgggcg
     1261 cctagctctg tcttcacagt cttagttcgt cgtatgcggt ggttcatcgc atccgccacc
     1321 gtactaccga catgctaggt gtcttacgtg gcggaaatcg cggttcccgg atggggtccg
     1381 cacgttgcga acgcagaagt tgcaccagcg acacattagc caacaaggtc agagcgctga
     1441 gacctctcat gcgccacaga aggaccggat tcgtcgatca cctcatttcc gagatcgcca
     1501 cgtcccacga gttcggccca gaaatacttt ccaagaaaag catttacctc acttcaaagt
     1561 gcggacggtt tttcctttcg aaactaattc taagttgccg ttaggtcgac cctccctggt
     1621 tcacaaagca aggttcgtga ggcccgagca cagactgccc gttgccagcg atccaccggc
     1681 cgccaccagc ggcttcacgc agttcggatg cggctgcggc tggtttggcc aaagttctag
     1741 acggctgtca agggagtgct aaattcccgc tcatgactac gacaatcgag accatcgaaa
     1801 ctaccgattc ttccgatatc aaagaaagcc ggataggccg tcttcggatt gtggtgcatc
     1861 cgggttcagg ctccggttcg gaatgcaccg gcacatgcgg gtgcatgtgc gtgagagagg
     1921 acgatgggcc ctagaaccgc ctccggtagt gccttcccag gttagctctc gctccagtaa
     1981 ccagcgggag accgttgccc gctcattgct ggatgtcctg ccaggagcat tcgaggcgct
     2041 cgattaccag gcgccctttc tcatcgagaa gctggtgaaa gatcgctttg tcgacactcc
     2101 tgacgaggct tgcgcgcttt tcaccgaggt gaagcgctac ctggtcctga cccaggtcga
     2161 ccaaacgaag aactggaaga tgcactcgct gtgcgtggat gaggcttggc accaattcgt
     2221 acttttcacc gcggagtaca gcgggttctg caccaagtat ttcggttgct accgccacca
     2281 ctttccaagc aacgcgccga acgccggcct catgtacatc cccgaggcga cgttagccga
     2341 gttcggtgac cgataccgtg acatcttcgg cacggatttg ccgcaggtat gggacgactc
     2401 gagttgggta actccacacc ggcgtgtcgt gaaccgcgat tgcggacaag tggcgttgga
     2461 ctcggtcgac aggatggtcg ccttgatcga ccacgacggt cggcctcttc tcagtgtcag
     2521 cgaaaacgca cgggaagcgc tgcgtttcct tgtcagtgca ggcgccttct atgtccgtga
     2581 attgcccggc gacttgaccg atgaggagaa ggtcgctctg gtggccgcac tggtcaaact
     2641 aaaggtcctt cgtgtcggtt gacagagccg cgccgcttgg cgccgtgcgc cgtgcgatgg
     2701 cattggcgga atacagccca cgtctacccc cggacggggg acaaggccca ggagtgctca
     2761 gtgaagctcg atgacctgtg cgaactgttt gaccggcagg agttccccca atcgcacgtg
     2821 cgcgagcatc cagacaggga tgcagtcctt gctgcccgat cggcggtcac cgcggctgtt
     2881 cgtgacgatg agttcctcgt cgattgcgtg gcatacgagc tgacccggct taaacaaccg
     2941 ggtctgcgtc cggggttggt tccgttcttc atcttgcctg aatttggggt ccgctttgcc
     3001 cttggctact ggccgccagg tcgcagtgct ggggcgcacg aacacacggc ctggacgatc
     3061 acaggcgtct gtcacaatga gctgacagtc cagacgtacg accgcgatca gtcgtaccgc
     3121 catcagacgc tggtgccgaa gaatcgcttc gatgctcttg ctggacaagt cggcttcatc
     3181 tacgagccgt gcatccacga tccacgcaac ccgacggacc gctggtcgct gtcgcttcac
     3241 gtcagcagcc cgcgcgacgg tgagcagctg acagaccaag aacaatgtct gccaattctc
     3301 gatgaatact tcactcgcca gcgaactgat tatggcgctc cctacgagga ggttgtggcg
     3361 actcgacggc ggcagctcaa gatccgtgcg atcgccggat tcctcgcaca ggtcgatgcc
     3421 gttccggtgg cggacttgct cgagcggtgc gtccagcaag gttcctcagc aaccagccgc
     3481 ttcatccacg gcttgggcag aactgacctc atgaacggcg gtcggcctac ggcgcgcacc
     3541 ctcgctcgtt gccacgagag tttggttctc agctatcgag aaaccgacga ttttgttgct
     3601 cttggcgtcc agacacagcg tggttgggta gaagaattgg ccgtttcacg cgtcgcacgt
     3661 gaggcgattg ctttctgtgt tggcgcaccc cggttcgatg tgctcgagct accgggatgc
     3721 ctaaccgacg acgagcgatg ggcgatcgca gacgcattgg aagaaagcgg actgttcact
     3781 gttgccgcca gtgaatgaca tcaatgccga agccgtgggt gtagtgcagg gccaatccca
     3841 cacacaccgg ctcctcggca ccagcgcggc catggcggcg ctgctgatca tcgcggtcac
     3901 agtggccggc tgcgaagggc cgcatccgat cgcatccacg caaacaactt caccacagcg
     3961 cgcgacgacc tcgctggcgc cgctggtggc gtccgaccag ctggactcca tcctgctaac
     4021 cgtcgcggac gtcaacgccg ctatgggcgc ctcgggcatg catcccacca cgccgatcgc
     4081 tcatgcgcca cgggaggtgg tcgagaggac gctatccaat ccggactgcc tgggcgcctt
     4141 ctttgtcggc gagccgcccg cgtatcaggg gagcggatac accgcggcca gcatcgtgac
     4201 gctgcacgag atggtaaaca accccgacca ccgcctcggg caggcggtgg tgagcttccc
     4261 gtcggccgat cgcgcgctgg ccttcttgaa gacctcggtg ggcgaatggc gggcctgcgc
     4321 cggaaaaacg ctcgtcgcaa cccacctgga gcaggtctac cggtggaccg taggaaacct
     4381 ggtcggggat gccccggcga ttacgcagtt gaacacgctc gaaggcggca acggatacgt
     4441 ctgccagcac gtgctgcgcg cggtgttgaa tgtggtcctc gacgtcaatg cctgcggata
     4501 tcacatcagc gaccaagccg gccggatcgc cgaaaagatg gcgggctcgg ccacccggca
     4561 agcccactga cgggcgaccg ggcggcggac gggacgatag tgctcggccc gcgttggcgc
     4621 ccttgaggat tcatcccagg tctatcccaa cacgaatcta tccgaagatc cccgggaacc
     4681 cgctggcgaa cggccgcaag gaaagttaga taggcgcccg tgagaattcc gatttctgat
     4741 gcccgcactc gccggttccc gcccgcccat tcacgatgct gaacgacggc cgcggtttcg
     4801 aaacggcccg caacggaggg tcacaagcta gcgttttcgg cgtccgagtt cgtcgatgtg
     4861 gaatgccgaa atgcgttgat taaggccagt ccctatgccc gccatcctcc cagcccccga
     4921 tcagcgtgac gaccagccga ggctgccata catggctcgc tggctcgaac gggtcaccaa
     4981 acctgtcggt agcacgaagt atccagaatc caattaccaa gacgtgcaac aagagcactg
     5041 aggacaacga cacttgacca gcaggacatc agtagacgta ttagtcgtcg gcacctcgat
     5101 tgacccccat atcgaggccg tcacttcgct cctgcctgga cagctcgtgg ttgcccggct
     5161 cgacgtcgac cagtatccgt gcgacagagc gctgacgata tcgctgagct catgccaccg
     5221 acacatcgac ctggagcagg gccaggaccg tgtcgatctc accagcgcaa aagtggtttg
     5281 gtttcgtcgg ttcggaccac cggtgctggc caccgatgtg cccagcgaat atcgcaggtt
     5341 ttgtctggcc gaggccgagc atctgctcga tggcgtgatt tcgctgcttc ggccctcagt
     5401 ctggatcaac gagtactggt caacgcgacg cgcgtcgact aagccttatc agtactcggt
     5461 agccgctgat gtcggctttg ttacgccgca gacgttgatc acgaatagcg cgtcctccgc
     5521 ggcggaatgg atagcgaaca gatccgaaat agttgcaaag acactgtcca cgccaattat
     5581 cacagatgac gatgatgaac ggggcttcgc tttaacacat gtcttgtcgg acagcgacaa
     5641 gtctgatcta caaccggtct cggtcacggc aacacagttt caggacctga tagatgtcgg
     5701 ctatgagctt cggatcacaa gtattggcga caagaatttt gcggtacgca ttgacagtga
     5761 ggccgctgaa gtgcagggcg tcagagattg gcgaagcgag aaaatcaaat cgaggtactc
     5821 atggtgcgag ttgccaagcg acgtcgacga gatgctcaca cggctccacc gcgcactggg
     5881 cctgagcttc gcagccagcg acttgatcgt cgatgagcat ggagaacact actttctcga
     5941 aaccaatcca cacggtaggt ggaggtggtt agagaaagcg cttggttctt cgcccataac
     6001 cgagtacttc gccgaatata ttactaaggg cacgatggca ggaatctagt acgaattgca
     6061 tcaggattgt cgctctgcgt cagccatatc tcagcgccgc cgcgaaaaat tagagctgtc
     6121 cctgtgagtg atcaattgcc gtgcaacgtc ggtcgccccg gggcttcaat tcagccaacg
     6181 atgccgtgga atcggacact gctgtgggca ctttttcgga gtctccgcga ttgtgcggtt
     6241 tcggatgcga atcgcccgga gatcgtcgga atgcgcacag tcacccaacc aatgtttgtg
     6301 gtttcaggac ggcgcgcggt tcacccgacg tcgacggctc tgcggcagtc atgctcgccg
     6361 ccgttgggtt cggtatatcg atccgagtct tctaagcgcg ttgaggattg tggcctcgga
     6421 aatagctccg tagccaagta tgagcgccgg tggcgcggac tgctgttcgg accagtgcca
     6481 gctggctcct tcgataagaa gacctcgcgt acgggctgca tcgaggagac gcgcttcgtc
     6541 acaccagtca ggcagctgga cgtacagatg caggcccgcg gcgatgccct ttggcacggc
     6601 cgcgggcagg gatgtcgcga ccgcctcgag catggtgtcg cgccggcgcc gatagatcgg
     6661 tcgcacccgc cgcaggtggc gggtaaagcc cccggtctcg ataaaccgcg cgagcgaaag
     6721 ttgttcagcg actgtggttc ccatgtcatc gaggagcttc tggtgagcga cgccgtcgac
     6781 tagccagcgc ggcagaatca tccacccgag tcgtagtgcc gggctcagtg ttttgctgat
     6841 gcatccggcg tagaccaccc gctcaggtgc gagtccctgt agtgcaccga gggacacgcg
     6901 gtcatagcga aactcggcgt cgtagtcgtc ctcgatgacc agcccgttgt tgtcgtgagc
     6961 ccatttgagc agcgcagcac gcctc
//
LOCUS       DASYLX010000300         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3193546,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000300 DASYLX010000000
VERSION     DASYLX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193546"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGP27_07300"
     CDS             <1..714
                     /locus_tag="VGP27_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7851155.1"
                     /translation="NWLAMHCRRHMELYGTTKEQLGWLAVNSRRNAGLNPRAAYREPM
                     TMDHYLSSRPVSTPFGLFDCDVPIDGSIALIVSHADYAADSPKPAVAVEAIGGTYGSG
                     GWFHRDDFPKMASVEAAAELWSRTDLTAADVDVAELYDGFTFLTFAWLEALGFCADGE
                     AGPFVDGAMRIALDGALPLNTYGGQLSAGRMHGYWLLHEACLQLRGEAGERQLATRPE
                     VAVAAAGGGPIAGCMLLTC"
     gene            832..2061
                     /locus_tag="VGP27_07305"
     CDS             832..2061
                     /locus_tag="VGP27_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEV7851156.1"
                     /translation="MGAGQGSAAWLPHSIAEAALAGAHGGQGVDLAAAWSHLLHKLED
                     AAQIVDADSACRNRLDRAAGMRHLLVLLAAGVDEALRFDPDPILRVQRTSTDDIVTWG
                     MECPDCLYTRAVMRGGERYRLFGNRGTARYVGLQTMNGIASTANALVDELDVEANGDF
                     EVVLSAEQRDGNWMRIDGAHPTLTVRHFFYDWDTEVPSSLHIERLGDPVETGDLAVDA
                     DVALSRQLVALGDFVNDNVEFFLQFGAAAPANGFLPPIDRTAMGAAAENRPVIGRWEL
                     EPDEALILEVEPPGGVYWSFSLGNPWWETIHYGRHQSSLNANQAVVDSDGLVRVVLSG
                     RDPGVANWLDTAGHSNGAVILRCVRTAAAPIPRARLVSFDDVASALPADTRRVAADER
                     AFTIDRRRRAVLERFAL"
     gene            2058..3203
                     /locus_tag="VGP27_07310"
     CDS             2058..3203
                     /locus_tag="VGP27_07310"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7851157.1"
                     /translation="MTFSADALEDGARAATGLHDFGSGYYREGLERTVEALNTEAHLS
                     DMGGVIQHATISNALIQRLKIEDAYANHPEIDDEIIGGPVFVIGLPRTGTTALSQLVA
                     ADPQFRSLRMWESQAPVPPPEAATQHTDPRIAQAEAGLQMLNDMFPLMKTLYNSEATA
                     PTECQDLMGMSFRTFHFDGVVRVPSYLAWLMDCDMRETYTFHRRVLKLLQWHCPPTLW
                     HLKTPVHVFALDALVSAYPDAKFLWSHRDPAKVMGSVCSLIRYVRSWSSDRDDAGELG
                     AEQLESWSEGIRRAMDFRHRVGDDRFADIAFADLQADPVNTLAAGYRSLGLEFTDATG
                     RMVEQWAHEHKPGSRGSHDYDLADYGLTPESVRERFAEYLRTYDATA"
     gene            3200..3862
                     /locus_tag="VGP27_07315"
     CDS             3200..3862
                     /locus_tag="VGP27_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851158.1"
                     /translation="MSRPATRRREKLAPDPSIRRAILVAASESVREQGVRGLSVAAVL
                     DRAQLSTRAFYRHFESKDQLVAAVFLEMAHAEMRRLRRQMVKAADPVEAVATWIDARL
                     DLAFDEGVESDLRRLSTEAQSQMFASPELIQPAYTAMLEPLIEQLQRGRAHGVFTDID
                     PMTEAQSIQGVVWASTERQWATGGCVRAAVRERALRFCLRGLGVAPKAIARIAGQTAA
                     FD"
     gene            3880..4683
                     /locus_tag="VGP27_07320"
     CDS             3880..4683
                     /locus_tag="VGP27_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851159.1"
                     /translation="MDLGFAGSKAVVTGGSKGMGLAIAEALAAEGASVAVMARNRAAL
                     DDAVEAIRAAGAPEAFGISVEMADAASIEAAFTAVSERWGQLNSLVHTIGPGDGYFEQ
                     MNDAGWEAAFTLGTMSGVRSIRASLPLLRAADWARIVTLSAHSIQRQNPRIVAYTASK
                     AALSSVTKNLSKSLAKDGILVNCVCPGTIVTASFTENLRDILAADGLDATDPRDVMTW
                     IDNNFHQPCDLGRAGLPEEIASITAYLASQRNGYVTGATVNVDGGSDFV"
     gene            4750..5427
                     /locus_tag="VGP27_07325"
     CDS             4750..5427
                     /locus_tag="VGP27_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851160.1"
                     /translation="MPTVTWARVDPSRRAAVVEAAEAEFGAHGFSQGSLNVIARRAGV
                     AKGSLFQYFADKRDLYAYIADIGSQRVRSFMEHRIRELDPSRPFFEFLTDLLDTWVAY
                     FADHPQDRALHAASSFEVDTEARVSVRTVINRHYLEVLRPLVRDAHIRGDLRADCDTD
                     ALLSLLLMIFPHMALAPYVRGVDPILGLDDPTPEQPALAVRRLVAVLADAFSSTSPAQ
                     LIKEVTQ"
     gene            5430..>5879
                     /locus_tag="VGP27_07330"
     CDS             5430..>5879
                     /locus_tag="VGP27_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV7851161.1"
                     /translation="MTRTRSASLAAGGLKWDSLPLKLFAGGNAKFWNPADIDFSRDRA
                     DWEALTELEREWATRLCAQFIAGEEAVTQDIQPFMAAMRAEGRLGDEMYLTQFAFEEA
                     KHTQVFRLWLDAVGVTDDMQVFLDDLPTYRKIFYEELPESLDTLATDP"
BASE COUNT         1047 a         1933 c         1893 g         1006 t
ORIGIN      
        1 aactggctgg ccatgcactg caggcggcac atggagctct acggaacgac caaggagcag
       61 ctgggttggc tggccgtcaa cagcaggcgc aatgccgggc tcaacccgcg cgcggcctat
      121 cgcgagccga tgacgatgga ccactacctg tcgtcacgtc cggtgtccac cccgttcggg
      181 ctgttcgact gcgacgttcc gatcgacgga tccatcgcgc tgatcgtctc gcatgccgac
      241 tacgccgccg acagcccgaa gcccgcggtg gcggtggagg cgatcggtgg cacatacggt
      301 tcgggagggt ggtttcaccg cgacgacttc ccgaagatgg cttcggtgga agcggccgcc
      361 gaattgtggt cacgcacgga cctcaccgcg gcggatgtgg acgtcgccga actctacgac
      421 ggtttcacgt tcctgacgtt cgcgtggttg gaggcgctgg gcttctgcgc cgacggcgag
      481 gcgggcccgt tcgtcgacgg ggcgatgcgg atcgcgctcg acggcgccct gccgctgaac
      541 acctatggcg ggcagctgtc ggcggggcgc atgcacggct actggctgct gcacgaggca
      601 tgcctgcagc tgcggggcga ggcaggcgaa cggcaactgg cgacccggcc ggaagtcgcc
      661 gtcgcggcgg cgggcggcgg cccgatcgcc gggtgcatgt tgctgacctg ctgacgccat
      721 cacgtaagcg agcattgaca tcgtccacgc ccggctgtga tagttgggac tccttaaacg
      781 aaaactatgt tttcatttaa gccggatcag actcgcatag gcggtaaaga agtgggcgct
      841 gggcaagggt cggcggcgtg gctgccgcac tccatcgcgg aagcggcgct cgccggcgcg
      901 cacggcggcc agggcgtaga cctggccgcc gcctggtcac acttgctcca caaactcgag
      961 gacgcggcac agatcgtcga cgcggattca gcgtgtcgga accggcttga tcgagcggcg
     1021 ggaatgcgcc acctgcttgt gctgcttgca gcaggcgtcg acgaagccct gcgcttcgat
     1081 ccggatccga tcctgcgtgt ccagcgcacc agcaccgacg acatcgtgac gtggggaatg
     1141 gagtgtccgg actgcctgta cacgcgcgcc gtcatgcgcg gcggtgaaag gtatcgattg
     1201 ttcggcaatc gcggcaccgc gaggtacgtc ggacttcaaa ccatgaacgg catcgcttct
     1261 accgcaaatg ctttggtaga cgaactcgac gtggaagcca acggtgactt cgaagtcgtg
     1321 ctgtccgccg aacagcgcga cggcaactgg atgcggatcg acggcgcgca tcccaccttg
     1381 acggtgcgac atttcttcta cgactgggac accgaggtgc cgtcgtcgct tcacatcgaa
     1441 cgactcggcg atccggtcga gaccggcgac ctggcagtcg acgccgacgt cgctttgtcc
     1501 cggcaactgg tcgcgctcgg cgacttcgtc aacgacaacg tcgaattctt tctccaattc
     1561 ggcgcggcgg caccggccaa cggcttcctg ccaccgatag accgcaccgc gatgggcgct
     1621 gcggccgaga acaggccggt catcggacgc tgggagctgg aacccgacga ggcgctgata
     1681 ctcgaggtcg agccacccgg cggcgtctac tggagttttt cgctcggcaa tccatggtgg
     1741 gagacaattc attacggtcg gcaccagtcc agcctgaatg ccaaccaagc cgtcgtggac
     1801 tccgatgggc tggtgcgggt ggtcctcagc gggcgcgacc ccggggtcgc caactggctc
     1861 gacaccgcgg ggcacagcaa cggcgcggtg atcctgcgct gcgtgcgcac cgcggcggcg
     1921 ccgataccgc gcgctcggct ggtctctttt gacgacgtcg cgtcggcgct gcccgctgac
     1981 accagaaggg tcgccgcgga cgaacgcgcg ttcaccatcg accgacggcg gcgtgcggtg
     2041 ctcgagaggt tcgcattgtg acgttttccg ccgacgcact cgaggacggt gcccgcgcag
     2101 cgaccggatt gcacgacttc gggtcgggct actaccgcga gggtctcgag cgcaccgtgg
     2161 aggcgttgaa caccgaagcc catctttccg atatgggcgg ggtcattcag cacgccacca
     2221 tcagcaacgc cctgatccag cggctgaaga tcgaggacgc ctacgcaaac caccctgaga
     2281 tcgacgacga aatcatcggt gggccggtct tcgtcatcgg cctgccccgc acgggaacca
     2341 ccgcgctgag ccagttggtg gccgccgacc ctcagttccg ttcattgcgg atgtgggagt
     2401 cgcaagcccc tgttcctccg ccggaggcag ccacccagca caccgacccg cgtatcgcgc
     2461 aagccgaggc gggcctgcag atgctgaacg acatgttccc cttgatgaag acgctttaca
     2521 actccgaggc cacggcaccg accgagtgcc aggacctgat gggaatgagc ttccgtacct
     2581 tccacttcga cggtgtcgtt cgcgttccga gttatctggc gtggctgatg gactgcgata
     2641 tgagagagac gtacacgttt caccgccggg tgctcaaact ccttcagtgg cactgcccgc
     2701 cgaccctatg gcatctcaag acgccggttc atgtgttcgc actcgatgcg ctcgtctcgg
     2761 cgtacccgga cgcgaaattc ttgtggagcc atcgcgatcc cgcgaaggtg atgggctcgg
     2821 tgtgcagcct gatcaggtat gtacgcagct ggagcagcga tcgtgacgac gccggtgaac
     2881 tgggggcaga acagctcgaa agctggtccg aggggatccg ccgggcaatg gacttccgtc
     2941 atcgtgtcgg cgacgatcgc ttcgccgata tcgccttcgc cgacttgcag gccgacccgg
     3001 tgaacacatt ggcggccggc tatcggtccc tcggcctcga attcaccgac gccaccgggc
     3061 gcatggtcga gcaatgggcc cacgagcaca aaccaggctc ccgcggatca cacgactacg
     3121 acctcgccga ctatggtttg accccggagt cggttcgtga acggttcgcc gaataccttc
     3181 gcacctacga cgcgacagca tgagccggcc agccacgcgg cgccgggaaa aactcgcacc
     3241 ggacccaagc atccgccggg cgatcctcgt cgccgcgtcg gaatccgtcc gcgagcaggg
     3301 agtccgcgga ctcagtgtcg ccgcggtgct tgatcgcgca cagctaagca cgcgtgcctt
     3361 ctaccgccac ttcgagtcga aagaccagct ggtcgcagca gtgttccttg agatggcaca
     3421 tgctgaaatg cgaaggctgc gaagacaaat ggtgaaggcc gccgacccgg tcgaagccgt
     3481 ggccacctgg attgacgcga ggctggacct ggccttcgac gagggcgtgg agtccgacct
     3541 gcgccggctg tcaaccgaag cgcagtctca gatgttcgcc tcccccgagc tgattcaacc
     3601 ggcttacacc gcgatgctcg aaccgctcat cgagcagctg cagcgcggcc gcgcccacgg
     3661 tgtgttcacg gacatcgatc cgatgaccga ggcacagtcc attcaaggag ttgtgtgggc
     3721 gagtaccgaa agacaatggg cgacaggcgg ttgcgtgcgt gccgccgtcc gcgaacgtgc
     3781 tcttcggttc tgtcttcgcg gactgggcgt cgcgccgaaa gcgatcgctc ggatcgccgg
     3841 gcagacagct gccttcgact gagaacctga ggagactcga tggacctggg attcgccgga
     3901 tcgaaagccg ttgtcaccgg cggcagcaag ggcatgggcc tggccatcgc cgaagccttg
     3961 gccgccgagg gcgccagcgt ggccgtcatg gcccgaaaca gggctgcctt ggatgacgca
     4021 gtggaagcca tccgggctgc gggcgccccg gaggcgttcg gaatcagcgt ggagatggcc
     4081 gatgcggcct ccattgaggc cgccttcacc gcggtctccg agcgctgggg acagctcaac
     4141 agcctggtcc acaccatcgg gcccggcgac gggtatttcg agcagatgaa cgatgcggga
     4201 tgggaggcgg ctttcactct gggcaccatg tccggcgttc gttccatccg cgcgtcgctt
     4261 ccgttgctgc gggcggccga ctgggcgcgc atcgtgacgc tctccgcgca ctccattcag
     4321 cggcaaaacc cgcgcatcgt cgcttacacg gcatcgaagg cagcgctgtc cagcgtcacc
     4381 aagaacctgt cgaagagcct cgccaaggac ggcatcctgg tgaactgcgt atgccccggc
     4441 accatcgtga cggcgagctt caccgagaat ctaagggaca tcctcgccgc cgacggactc
     4501 gacgccaccg acccacgcga cgtcatgacc tggatcgaca acaactttca tcagccttgc
     4561 gacctcggcc gtgccgggct gcccgaggag atcgcctcga tcactgccta tctggcatcc
     4621 cagcgcaacg gctacgtcac cggagcaacc gtgaacgtcg acggcggctc ggatttcgtc
     4681 tagacactct tgacggtgac gtggctcaca gtattatgac cagtggtcat cgactgttgg
     4741 gaggtcacga tgccgacggt gacatgggct cgcgtcgatc cgagccgccg ggccgccgtg
     4801 gtcgaagccg cggaagccga gttcggtgcc cacggttttt cgcagggcag cctgaacgtc
     4861 atcgcgcggc gcgccggagt cgccaagggc agtctgttcc agtacttcgc ggacaagcgc
     4921 gacctctacg cgtacatcgc cgacatcggc agtcagcgcg tgcgctcgtt catggaacac
     4981 cgcattcgtg agctcgaccc gagtcggccg ttcttcgaat tcctgaccga ccttctcgac
     5041 acctgggtcg cctacttcgc cgaccatccg caggaccgtg cgctgcacgc cgcgtcgagc
     5101 tttgaagtcg acaccgaggc ccgcgtcagc gtgcggactg tcatcaaccg ccactatctg
     5161 gaggttctgc gaccgctcgt acgcgacgcc cacatccgtg gcgatctgcg cgcggactgc
     5221 gataccgacg cgctgctgtc actgctgctg atgatcttcc cgcacatggc gttggcacca
     5281 tacgtgcgcg gcgtggaccc gattctcggc ctcgacgacc cgacacccga acagcccgca
     5341 ctcgcggtgc gcaggctcgt cgccgtgctc gcggacgcct tctcttccac cagcccagcc
     5401 cagttgatca aggaggtcac acaatgacac tgacacggac acgctcggcc tcactggccg
     5461 caggcggcct caagtgggat agcttgccgc tcaagctttt cgccggcggc aacgccaagt
     5521 tctggaaccc cgcggatatc gatttctcgc gtgaccgggc ggactgggag gcgctcacgg
     5581 agctggagcg cgaatgggcg acccggctgt gcgcgcagtt cattgccggc gaagaggccg
     5641 tcactcagga catccagccg ttcatggcgg cgatgcgggc ggaagggcga ctcggcgacg
     5701 agatgtacct gacccagttc gccttcgagg aggccaaaca cacccaggtg ttccgcctgt
     5761 ggctcgacgc cgtcggcgtc accgacgaca tgcaggtctt cctcgacgac ctgcccacct
     5821 accggaagat cttctacgaa gagctgccgg aatccctcga cacgttggcc accgacccc
//
LOCUS       DASYLX010000301         8442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2865405,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000301 DASYLX010000000
VERSION     DASYLX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865405"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGP27_07335"
     CDS             <1..489
                     /locus_tag="VGP27_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7851162.1"
                     /translation="VIASDDAVIGTPYSRMWGAYLSGMWLYRMSFAKAKWHSLTGEPL
                     TGREAADVELINESVPFDRLEARVAEVAAMLCRIPLSQLQAQKLVVNQAYENMGLAST
                     QTLGAILDGLMRNTPDAQQFIETAATEGVRAAIEHRDGPWGDYSQAPPERRPDPSHVI
                     EP"
     gene            complement(476..1456)
                     /locus_tag="VGP27_07340"
     CDS             complement(476..1456)
                     /locus_tag="VGP27_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEV7851163.1"
                     /translation="MPFVTDIQGPQRPVRTWPPPVTGALRRTSTIDTHPAQAGNSAVD
                     LRARDAVAGADAIDVLGQVAARAHLAERVIDEIVSDPPDGRLAQLTGARVGPGFRSTV
                     ARLFPDQVRRAGLLQLLLDDWVGAALVSGYAVQHAAINLGIEETLPPGTADHMAGICA
                     GFASDASLVPYARRNGTIPSVHGPVAPRFPANGMHPVEPLRPHGMRRFRRLDLAPDGG
                     FDAHFRDSHVDGDGVETIVHEYTVEGTVDAATRTITSVTATVRVLPWQECPAAIGSAV
                     RVKGMMLSELRNRVRGEFVGTSTCTHLNDTLRAIADLDALLDLRAGTRAR"
     gene            complement(1466..2320)
                     /locus_tag="VGP27_07345"
     CDS             complement(1466..2320)
                     /locus_tag="VGP27_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851164.1"
                     /translation="MRFLAALLFWLVTTVLLAVAVPVSWAQKNVVSEDGYAALAATAA
                     ENPRLQQAMASELTTQITELAADNGYALNNAELVRGVTGAYTANSGFPRQFAQANRIA
                     HRWMFTDSVARDQGSGDRWLVDIAPMLSDDSLRQTLGHLNLDVPDTLMVPITVPENSS
                     LRPGQLKPLSTWGPWVSIGACVLTGVFALLTVATARSRGKALAALGVSALLVGAAGWA
                     GLEVGRRYIDDALNRTTGDIRQVAEVMVNTAEGSSHQWLNLTLAAGGVLVVLGIFVSM
                     LGGLRRSD"
     gene            complement(2468..3988)
                     /locus_tag="VGP27_07350"
     CDS             complement(2468..3988)
                     /locus_tag="VGP27_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7851165.1"
                     /translation="MTATPEVDSSSSTTTIRNPATGAVAGQVRWTDPADVPRIATGLR
                     EAQREWEARGAKGRAKVLARYAVWLGEHRDEIESLLIKETGKSAVDAAQEVPLLIMIL
                     SYYIKTMEKALAPETRPASLPFLAIKKVTVHFRPRQVVGIIAPWNYPVANALMDAIGA
                     LAAGCSVLLKPSERTPLTAEVLLRGWQDSGAPEVLALAQGAREVSEAVIDNSDFIQFT
                     GSSATGAKVMERAARTLTPVSLELGGKDPMIVLDDADIGLAAHAAVWGAMFNAGQTCV
                     SVERVYVLDSVYDQFVDAVVRDVEKLKIGAGQGYDFGALIDDDQVAVTERHVRDAVAA
                     GARALTGGERTAGQGSFYPPTVLVDVDHSMACMTEETFGPTLPIMKVSTVGEAVRLAN
                     DSPYGLSASVFSKDIERAEDVAVQLDCGAVNVNDVISNLMCTTAPMGGWKMSGLGARF
                     GGAEGLRKYCRQEAVVAPRTNLGAGGNYYNNSHKALTRMNKLMTRLALLRPRRTAK"
     gene            complement(3999..4583)
                     /locus_tag="VGP27_07355"
     CDS             complement(3999..4583)
                     /locus_tag="VGP27_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase PA-phosphatase"
                     /protein_id="HEV7851166.1"
                     /translation="MRNPAGRRALLLWPVLAVAAMLVLGAAVGKRSTPLDDWFHRFRH
                     TPARELLVFSDPYLLIFVVCVGFLVALSLRRWRLAVVVICSPLVGVFLARVLKQLFGR
                     ENDGALAYPSGHTTAVVVVSGMIALVAGGAMWAVLAAIAFSVLAMIGQAVTYHYFTDT
                     VGALLLGSAVVWLAAAVAKLDRRQPGCDPDHTAG"
     gene            complement(4585..5103)
                     /locus_tag="VGP27_07360"
     CDS             complement(4585..5103)
                     /locus_tag="VGP27_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol transferase"
                     /protein_id="HEV7851167.1"
                     /translation="MADSDVQAVRELLRDSFTRLIEHVDDLTDGLTDEVSFFRPTAQA
                     NSIAWLLWHSARIQDLQICDIAGIEQVWIRDGWDDRFGLDLGGNDHGYGHDPDDVGKV
                     RAPADLLAGYYHAVHKVTLEYIASVSGEELSRVVDTHWTPPVTAGARLVSIIDDCAQH
                     LGQAAYIRGIAT"
     gene            complement(5142..5522)
                     /locus_tag="VGP27_07365"
     CDS             complement(5142..5522)
                     /locus_tag="VGP27_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851168.1"
                     /translation="MIDHLGINCADWEKSKTFYDEVLGVLGYTRQLDYQVAIGYGKDG
                     KPDFWIADMSVGEASGANREVHVAFHARDHDAVQAFYDTALRLGAESLHAPRLWPEYH
                     PGYYGAFVRDPDGNNVEAVFHGAP"
     gene            5569..7302
                     /locus_tag="VGP27_07370"
     CDS             5569..7302
                     /locus_tag="VGP27_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851169.1"
                     /translation="MPYELIVRNGTIVDGLGGEPFTGDVAIADGVIAAVGDVEGEAAK
                     EIDATGLLVTPGFVDLHTHYDGQAIWSDRMTPSSAHGVTTAVMGNCGVGFAPCRSEDH
                     EVLVDVMAGVEDIPGVVMVDGLPWTWETFPEFLDAVASRQRDIDVAAFLPHSPLRVYV
                     MGRRGVDREPATAEDLSQMRKLAAEAIDVGALGFASSRLTIHKTESGHPIPSYDAGHD
                     EIEAIARGVADAGGGLIQFVPDLVAGDYGPALQTVFDVAADVGLPVTFTLAIGNAGPA
                     FFEDAMTMVEKANSNAAAAEPVVTAQVFPRPIGLVIGLELSGNPFVMYPSYREIAGLP
                     LAERVAEMRKPQVRERILSDKPASDGHPLMFAVQAFDWIFPLGDPPDYEPDPSNSIGA
                     RAKARGVSPLEEAYDRLLDDDGHAMLLVTLANFRDASLDTVAELVRREDVVLGLGDGG
                     AHYGMICDASFPTYMLTHWARDRASGRLTVAEAVRELTSVPARVAGLADRGRIGVGYK
                     ADVNVVDHDALRLHKPIVSYDLPAGGRRLDQSADGYVATIVSGQVIAENGVPTEARPG
                     RLVRGRQPAPA"
     gene            complement(7346..8173)
                     /locus_tag="VGP27_07375"
     CDS             complement(7346..8173)
                     /locus_tag="VGP27_07375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851170.1"
                     /translation="MPLPQPSPTSTAVVTGASSGIGADIARELVARGHGVTLVARRED
                     KLRDLAADLGTTVRIEVIACDVADPDARAGLFDAVADRGLTIDILVNNAGVGTIGSVT
                     MTPVADEIAQVRVNVEAVIDLTTRAVQLMVPRGRGAILNVGSTVGYYPFPGQAGYAAT
                     KAFVKTYTEGVRGELAGTGVTVAMLAPGPVRTEFLQAAGMDEDKFAASFPKFLWMPSR
                     DVARIGVQALENDSGIVIAGKRNVLSTRFMQALPRPVLLSTLRKRHPGLRRDRRSVG"
     gene            complement(8205..>8442)
                     /locus_tag="VGP27_07380"
     CDS             complement(8205..>8442)
                     /locus_tag="VGP27_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7851171.1"
                     /translation="RYANWAVANNSLLIITFDEDDGGSKNQIPTIIYGAHVRPGTYSE
                     PISHYNVLSTIEEMYGLPKSGLAANAPAISDIWA"
BASE COUNT         1353 a         2869 c         2895 g         1325 t
ORIGIN      
        1 gtgatcgcca gcgacgacgc cgtcatcggc acgccgtact ccaggatgtg gggcgcctac
       61 ctgagcggga tgtggctgta ccggatgagc ttcgcgaagg cgaagtggca ttcgctgacc
      121 ggggagccgt tgacgggccg cgaagccgcc gacgtcgagc tgatcaacga atcggtcccg
      181 ttcgaccgac tggaggcgcg cgtcgccgaa gtggccgcca tgctttgccg aatcccgttg
      241 tcccagttgc aggctcagaa gctggtcgtc aaccaggcgt acgagaacat gggactggcg
      301 tcgacccaga cgctgggcgc catcctcgac gggctgatgc gcaacacccc ggatgcacag
      361 cagttcatcg agacggccgc caccgaaggg gtccgcgccg cgatcgagca ccgcgacggc
      421 ccgtggggcg actacagcca ggcgccgccg gagcggcggc ccgacccgtc gcacgtcatc
      481 gagccctagt gccggcccgc agatcgagca gcgcatcgag gtcggcgatc gcgcgcaagg
      541 tgtcgttgag atgcgtgcag gtgctggtgc cgacgaactc gccgcggacg cgattcctca
      601 actccgacag catcattcct ttgactcgga cagcgctgcc gatggctgct ggacattctt
      661 gccacggcag cacccgcacc gtggccgtca ccgacgtgat cgtcctcgtc gccgcgtcga
      721 ccgtgccctc gacggtgtac tcgtgcacga tcgtctcgac accgtcgccg tcgacgtggg
      781 agtcgcggaa gtgtgcgtcg aaaccaccgt ccggggcgag gtccagtctc cggaaccgcc
      841 gcatgccatg cgggcgcagc ggctcgaccg ggtgcatgcc gttcgcagga aaccgcggcg
      901 ccaccggacc gtgcaccgac gggatggtgc cgttgcgcct ggcgtaggga acgagtgatg
      961 cgtcggatgc gaagcccgcg cagatgcccg ccatgtggtc ggcggtgccg ggcggcagcg
     1021 tctcctcgat gccgagattg atcgccgcgt gttggacggc atagccggac accaacgccg
     1081 cgcccaccca gtcgtcgagc agaagttgca gcaggcccgc ccgccggacc tggtcgggaa
     1141 acagcctggc aaccgtcgag cggaacccgg gtccaacgcg cgcccccgtc agctgcgcca
     1201 gccgtccgtc gggcgggtcg gagacgatct cgtcgatgac ccgctcggcc aggtgtgcgc
     1261 gcgcagcgac ctgccccagc acgtcgatcg catcggctcc ggcgaccgcg tcgcgggccc
     1321 gcaggtcgac cgcggaattg ccggcctgcg ccgggtgggt gtcgatggtg ctcgtccgcc
     1381 gcagtgcacc ggtcacgggc ggcggccacg tccggaccgg ccgctgtggc ccctggatgt
     1441 ccgtgacgaa tggcatggcg agactctagt cgctgcggcg caacccgccg agcatcgaga
     1501 cgaaaatgcc gagcaccacc agtacaccgc ccgcggccag cgtcaggttc agccactggt
     1561 ggctgctgcc ctcggccgtg ttcaccatca cctctgccac ctgacggatg tcaccggtcg
     1621 tgcggttcag cgcatcgtcg atgtagcgcc tgcccacctc cagcccggcc caccccgccg
     1681 caccgaccag aagagccgaa acacccagcg cggcaagcgc tttaccccgg gaccgcgccg
     1741 tcgccaccgt cagcagcgcg aacacccccg tgagtacgca ggcaccgatg ctcacccacg
     1801 gaccccacgt cgacaacggc ttcaactggc ccggccgcag cgaggagttt tcgggcaccg
     1861 tgatcggcac catgagcgtg tccggcacgt cgagattgag gtggccgagc gtctggcgaa
     1921 gcgagtcgtc ggagagcatc ggtgcgatat cgacgagcca gcggtcgccg ctgccctggt
     1981 cgcgggcgac cgagtcggtg aacatccacc ggtgggcgat gcggttggcc tgggcgaact
     2041 gtcggggaaa tcccgagttg gcggtgtagg cgccggtcac gccgcgaacg agctcggcgt
     2101 tgttgagcgc gtacccgttg tcggcggcca gctcggtgat ctgcgtcgtc agctccgagg
     2161 ccatcgcctg ctgcagtcgg ggattctcgg cggccgtcgc cgccagggcg gcgtatccgt
     2221 cctcgctcac cacgttcttc tgcgcccagg acaccgggac cgcgaccgcg agaagaaccg
     2281 tcgtcacgag ccagaacagc agcgccgcca gaaaccgcac gctgtcctcc tacggggatc
     2341 gggcgcgcgc actggccgac cgaccacctc atggtccttg ttgtcgtagt cgaacttact
     2401 cagcacgtgc cgaatggcgt tgatgcgggc gcgcttcttg atcgacccgg cgaatggtga
     2461 agcggggcta tttcgcggtg cggcgcgggc gcagcagtgc cagccgggtc atcagcttgt
     2521 tcatccgggt cagcgccttg tgcgaattgt tgtagtagtt gccgcccgct ccgaggttcg
     2581 tgcgtggggc cacgacggct tcctgcctgc agtacttgcg cagtccctcg gcgccaccga
     2641 accgcgcgcc caagcccgac atcttccagc cgcccatcgg cgccgtggtg cacatcagat
     2701 tggagatcac gtcgttgacg ttgaccgcac cgcaatcaag ctgcaccgca acatcttcgg
     2761 cgcgttcgat atccttggag aagaccgagg cggacaggcc gtaggggctg tcgttggcga
     2821 gccggaccgc ctcccccacc gtcgacacct tcatgatcgg cagcgtcggg ccgaaggtct
     2881 cctcggtcat acaggccatc gagtggtcga cgtcgaccag caccgtcggc gggtagaagc
     2941 tgccttggcc tgccgtgcgc tcgccgccgg tgagcgcccg agcgcccgcg gccaccgcgt
     3001 cgcgcacgtg ccgctcggtc accgccacct ggtcgtcgtc gatcagcgcg ccgaagtcgt
     3061 aaccctggcc cgcgccgatc ttcagcttct ccacatcgcg cacgacggcg tcgacgaact
     3121 ggtcgtaaac cgaatcgagg acatacaccc gctcgaccga cacgcaggtc tggcccgcgt
     3181 tgaacatcgc accccacacc gcggcgtgcg cggcgaggcc gatgtcggcg tcgtccagca
     3241 cgatcatcgg atccttgccg ccgagctcga ggctgaccgg cgtgagagtg cgagccgccc
     3301 gctccatcac cttggcgccg gtcgcactgg agccggtgaa ctggatgaag tcggagttgt
     3361 cgatgacggc ttctgacacc tcgcgcgccc cctgtgccag cgccaacacc tcgggagcac
     3421 cggagtcctg ccagccgcgc agcagcacct cggcggtcag cggcgtgcgt tcggacggct
     3481 tcagcagcac cgagcagccc gcggccagcg cgccgatggc gtccatcagg gcgttggcca
     3541 ccgggtagtt ccagggcgca atgatgccga ccacctggcg cgggcggaag tgcacggtga
     3601 ccttcttgat cgccaggaac ggcagcgatg ccggccgtgt ctccggggcc agcgccttct
     3661 ccatcgtctt gatgtagtac gacaggatca tgatcagcag cggcacctct tgcgccgcat
     3721 cgacggccga cttacctgtc tccttgatca gcaacgactc gatctcgtcg cggtgctcac
     3781 cgagccagac ggcatagcgg gcaagcactt tggcgcggcc cttggcgccg cgcgcctccc
     3841 actcccgctg cgcttcgcgc aacccggtgg cgatgcgcgg cacgtcggcg gggtcggtcc
     3901 agcggacctg cccggcaacg gcgccggtcg cggggttgcg gatggtggtc gtagacgagg
     3961 acgaatcgac ttctggcgtc gcggtcatga cccccagctt agccagcggt gtgatctgga
     4021 tcgcagccgg gttgacgcct gtcaagtttg gccacggcgg cggccagcca aaccacggca
     4081 ctgcccagca gcaacgcgcc gactgtgtcg gtgaagtagt ggtaggtgac ggcctggccg
     4141 atcatggcca gcacgctgaa agcgatggcc gcgagcacgg cccacatggc accacctgcc
     4201 accaacgcga tcatgccgct gacgacgacg accgccgtcg tatgtccgct cggatacgcc
     4261 agcgcgccgt cgttctcccg tccgaacagt tgcttgagca ctcgggcgag aaagactccg
     4321 acgaggggag agcagatcac caccaccgcc agccgccacc ggcgcaggct cagtgcaacg
     4381 agaaaaccga cacagacgac gaagatcagg agataagggt cggagaacac cagcaactcg
     4441 cgcgcggggg tgtgccggaa tcggtggaac cagtcgtcca agggcgtcga ccgcttcccg
     4501 accgcggccc cgagcacgag catcgccgcg acggcgagaa ccggccacag cagaagcgct
     4561 ctgcggcctg ccgggttgcg cacgtcaggt cgcgatgccg cggatgtagg cggcctgacc
     4621 gaggtgctgt gcacagtcgt cgatgatgct gaccagtcgc gctcctgcgg tgacgggcgg
     4681 cgtccagtgc gtgtccacca cccggctcaa ctcctctccg gacaccgacg cgatgtactc
     4741 cagcgtgact ttgtgaacgg cgtggtagta gccggccagc aggtcggcag gcgcgcggac
     4801 cttgccgacg tcgtcggggt cgtgaccgta gccgtggtcg ttgcccccga ggtcgaggcc
     4861 gaaccggtcg tcccagccgt cgcggatcca cacctgctcg atgcccgcga tatcgcagat
     4921 ctggagatcc tgaatacggg cgctgtgcca gagcaaccac gcgatgctgt tggcctgcgc
     4981 ggtcggccgg aagaacgaaa cctcgtcggt cagaccgtcg gtcaggtcgt cgacgtgctc
     5041 gatcagtcgc gtgaaggagt cgcggagcag ttcgcggacc gcttgaacgt cggagtcagc
     5101 catgcgacag acgctacccg cgcaggcttg cgcagttagc tctatggtgc gccgtggaag
     5161 accgcttcga cgttgttgcc gtccgggtcg cggacgaagg cgccgtagta gccggggtgg
     5221 tattccggcc agagccgggg cgcgtgcagc gactcggcac ccagccgcag cgcggtgtcg
     5281 tagaacgcct gcacggcgtc gtggtccctg gcgtggaacg cgacgtgcac ctcgcggttg
     5341 gcgccgctcg cctcgccgac gctcatgtcg gcgatccaga agtccggctt gccgtccttg
     5401 ccgtacccga tggccacttg gtagtccagc tggcgcgtgt agccgagcac tccgaggacc
     5461 tcgtcgtaga aggtcttcga cttctcccag tccgcacagt tgattcccag gtggtcgatc
     5521 accgccccat cctggcatga cggtgcgccg gcgtcgtaga ttcggctgat gccctacgag
     5581 ttgatagttc gcaacggcac catcgtcgac ggcctcggcg gtgaaccctt caccggagat
     5641 gtcgcgatcg ccgacggtgt gatcgcggcc gtcggcgacg tcgagggcga ggcggccaag
     5701 gagatcgacg cgaccggcct tctggtcaca ccgggcttcg tcgacctgca cacccactac
     5761 gacggacagg ccatctggtc ggaccgcatg acgccgtcgt cggcacacgg tgtgacgacc
     5821 gcggtgatgg gcaactgcgg tgtgggcttt gcgccgtgcc gcagcgagga ccacgaggtg
     5881 ctggtggacg tgatggccgg cgtggaggac attcccggtg tcgtgatggt cgacgggctg
     5941 ccgtggacat gggagacgtt cccggagttt ctcgacgccg tggcgtcgcg acaacgggat
     6001 atcgatgtgg cggcgtttct gccgcactcc ccgctgcggg tctacgtgat ggggcggcgc
     6061 ggagtcgacc gagaacccgc caccgccgaa gacctgtcgc agatgcgcaa gctggccgcc
     6121 gaggcgattg acgttggggc gcttgggttc gcgtcgtcgc ggctgaccat ccacaagacc
     6181 gagagcggtc atccgatccc gagctacgac gcaggccacg acgagatcga ggccatcgcg
     6241 cggggcgttg cggacgcagg cggcgggctg atccagttcg tacccgacct cgtcgccggt
     6301 gactacggac ccgcgctgca gacggtcttc gacgtcgccg ccgacgtcgg gttgccggtg
     6361 acgttcacgc tggcgatcgg caatgcgggg cccgcgttct tcgaggacgc gatgaccatg
     6421 gtggagaagg ccaactcgaa tgcggcggcg gccgagcctg ttgtcaccgc acaggtcttc
     6481 ccccgcccga tcggcctggt gatcggactc gagctgtcgg gcaacccgtt cgtgatgtat
     6541 cccagctacc gcgagatcgc tggactgccg ctggccgagc gtgtcgccga gatgcgcaaa
     6601 ccgcaagtgc gggaacgcat tctgagcgac aagccggcgt cggacggtca cccgctgatg
     6661 ttcgcggtgc aggccttcga ctggatcttc ccgctgggcg atccgcccga ctacgagccc
     6721 gatccctcca acagcatcgg cgcaagagcc aaggcccgtg gggtgagccc gctggaggag
     6781 gcctacgacc ggctgctcga tgacgacggc cacgcgatgc tgctcgtgac gctggccaac
     6841 ttccgcgacg cctcgttgga caccgtcgcc gaactggtcc gccgcgagga tgtcgtgctc
     6901 ggcctcggcg acggcggcgc ccattacgga atgatctgcg acgcaagctt tcccacctac
     6961 atgctgacgc actgggcgcg ggaccgggca tcgggccggc tgactgtggc cgaggccgtc
     7021 cgcgagctga cgtcggtgcc cgcgcgggtc gcgggcctgg ccgaccgcgg ccggatcggc
     7081 gtcggctaca aggccgacgt caacgtcgtc gaccacgacg cgctgcgtct gcacaagccg
     7141 atcgtgagct acgacctgcc cgcgggcggg cgccgcctcg accagagtgc ggacggctac
     7201 gtggcgacga tcgtctcggg ccaggtcatc gccgagaacg gcgtacccac cgaagcgcgg
     7261 ccgggcaggc tggtgcgcgg ccgccagccc gcacccgcat gacgcggcgc cggccgctgc
     7321 gcaggtgagg agcgatcggg cgggctcagc cgactgagcg tcggtcgcgc cgcagccctg
     7381 gatgccgctt ccgcagcgtc gacaagagca ccggacgcgg aagcgcttgc atgaagcggg
     7441 tactgagcac gttgcgttta ccggcgatca cgatgccgct gtcgttctcc agcgcttgga
     7501 cgccgatgcg tgccacatcg cgcgacggca tccacaggaa cttcgggaac gacgcggcga
     7561 acttgtcctc gtccatgcct gccgcctgaa ggaactcggt acgcaccggg ccgggcgcga
     7621 gcatggccac ggtgacaccg gtgccggcca actcgccgcg cacaccctcg gtgtaggtct
     7681 tcacaaacgc cttggtggcc gcatagcctg cctggccggg gaacgggtag tagccgacgg
     7741 tggacccgac gttgaggatg gcgccgcggc cgcggggcac catcagctgc accgcgcggg
     7801 tggtcaggtc gatgacggcc tcgacattga cgcgcacctg cgcgatctcg tcggcgaccg
     7861 gcgtcatggt gaccgacccg atggtgccga cgcccgcgtt gttgacgagg atgtcgatcg
     7921 tcaggccgcg gtcggcgacg gcgtcgaaaa ggcctgcgcg ggcgtcggga tcggccacgt
     7981 cgcaagcgat cacctcgatg cgcacggtcg tgccgagatc ggcggcgagg tcgcgaagct
     8041 tgtcctcgcg gcgggcgacc agggtcaccc cgtggccgcg ggcgaccagt tcgcgggcga
     8101 tgtcggcgcc gataccggac gacgccccgg tgacgactgc ggtcgaggtg ggcgacggct
     8161 gcggaagtgg catggctcga acctaccccc ggtccgcctg ccggttacgc ccagatgtcc
     8221 gatatcgcag gcgcgttcgc cgccagtccg ctcttcggca ggccgtacat ctcctcgatc
     8281 gtggacagca cgttgtagtg gctgatcggc tcgctgtagg tgccgggtcg gacatgggcg
     8341 ccgtagatga tcgtggggat ctggttcttc gagccgccgt cgtcctcgtc gaacgtgatg
     8401 atcagcaggc tgttgttggc caccgcccag ttcgcgtagc gc
//
LOCUS       DASYLX010000302         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1549176,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000302 DASYLX010000000
VERSION     DASYLX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549176"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VGP27_07385"
     CDS             complement(<1..827)
                     /locus_tag="VGP27_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEV7851172.1"
                     /translation="MDSDIIFVLVVVTALAFDFTNGFHDTANAVATSISTGALAPRTA
                     VTLAAILNFVGAFISLQVAATVAGGIVDAHLVNTTVIFAGLIGAISWNLLTWYYGLPS
                     SSSHALIGGVVGAAFVAGGADAVFTDGLLSKVIVPAVVAPIVAFVAAGTAILITYRMV
                     GRLRPGPVNRGYRLGQIASASLLSLSHGTNDAQKTMGIITLALVAHGNLAGVNPPPPT
                     WVVVSAATAIALGTYSGGWRIIRTMGSRIFKMDPAQGFAAQSGGAAVLLTAAHLGFPL
                     "
     gene            complement(846..1472)
                     /locus_tag="VGP27_07390"
     CDS             complement(846..1472)
                     /locus_tag="VGP27_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEV7851173.1"
                     /translation="MARLSQVFAPKDRAFFDLFEESANNVLRAAELLDEMLHSYPEQA
                     QLARAILLCEQEGDRITYDIIQRLNSTFVTPIDRDDILNLASGLDDIVDLTEEVADYL
                     GLYRIEAPMEQAQRQAHILMEACRQISEAMPRMRGFRDVSHYTTEIHRLETDGDRVTR
                     EAVASLFDTGIDPMVVIRWKDIFERLEDAVDATEHIANILANIVDKNS"
BASE COUNT          249 a          492 c          549 g          237 t
ORIGIN      
        1 agcgggaagc cgaggtgcgc ggcggtcagc agcacggcgg cgccgccgct ctgcgcggcg
       61 aacccctgtg ccgggtccat cttgaagatg cggctgccca tcgtccggat gatccgccag
      121 ccgccggagt aggtccccag cgcgattgcc gtcgccgccg ataccaccac ccacgtcggc
      181 gggggcgggt tgactccggc gaggttgccg tgcgcgacga gcgccagcgt gatgatgccc
      241 atcgtcttct gtgcgtcgtt cgtcccgtgc gacagcgaca ggaggctcgc gctggcgatc
      301 tgaccgagcc ggtagccgcg gttgaccggt ccgggccgca ggcgccccac catgcggtac
      361 gtgatgagga tcgcagtgcc ggctgcgacg aacgcgacga tcggggccac gaccgccggc
      421 acgatgacct tcgagagcaa tccgtccgtg aagaccgcgt cggcgccgcc ggccacgaac
      481 gcagcgccga cgaccccgcc gatcagcgca tgggaggagc tcgacggcag gccgtagtac
      541 cacgtcagga ggttccagga gatcgcaccg atgagcccgg cgaagatgac cgtggtgttg
      601 acgagatggg cgtcgacgat gccgccggcg accgtcgcgg cgacctggag tgagatgaac
      661 gccccgacga agttcaggat cgccgccagc gtgacggccg tgcgcggcgc gagggcgccg
      721 gtcgagatcg acgtcgccac cgcgttggcc gtgtcgtgga agccgttcgt gaagtcgaac
      781 gccagcgcgg tcaccacgac gaggacgaag atgatgtcgc tgtccacgac ggcgtcgccg
      841 gcgcgctagg agttcttgtc gacgatgttc gccaggatgt tcgcgatgtg ttcggtcgca
      901 tcgacggcat cctcgagacg ctcgaagatg tccttccacc ggatgacgac catcgggtcg
      961 atgcccgtgt cgaacaggga ggcaacggcc tcgcgggtga cgcgatcccc gtcggtctcg
     1021 agccggtgga tctcggtcgt gtagtgcgag acgtcccgga agccccgcat ccgcggcatc
     1081 gcctccgaga tctggcgaca ggcctccatg aggatgtggg cctggcgctg ggcctgctcc
     1141 atcggcgcct cgatgcggta caggccgagg tagtcggcga cctcctcggt gaggtcgacg
     1201 atgtcgtcga ggcccgaggc gaggttcagg atgtcgtcgc ggtcgatcgg cgtgacgaac
     1261 gtcgagttca ggcgctggat gatgtcgtac gtgatccggt cgccctcctg ctcgcagagc
     1321 aggatcgccc gcgccagctg cgcctgctcg ggataggagt gcagcatctc gtcgagcagc
     1381 tcggccgcgc gcagcacgtt gttcgccgac tcctcgaaca ggtcgaagaa ggcgcggtcc
     1441 ttcggtgcga agacctgcga gagccgggcc atgggccgcg ccagtctacg tcaggcggtt
     1501 acggaacgaa gaaagctcat cctggtc
//
LOCUS       DASYLX010000303         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1877524,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000303 DASYLX010000000
VERSION     DASYLX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877524"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VGP27_07395"
     CDS             complement(<1..1043)
                     /locus_tag="VGP27_07395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7851174.1"
                     /translation="MAKLRFGYFIAPFHRAGTNPTLALQRDLEFVEHLDRVGFDEVWI
                     GEHHSAGSEIISSPEVFIAAAAQRAKRIRFGTGVISLPYHNPLWAADRLMLLDHLTHG
                     RIIGGVGPGSLPTDSSMIGLTPTDTRELLETNLDILVRLLAGETVTAKTATHELHDAQ
                     LQLAPYSEGGIPLSVAAVASPTGARLAGKHGIGLLSIGATLIVEGFDALAFHWGIAEE
                     RAAAFGTTVDRRNWSLVCPMHIAETEEQARDDVRFGIASWFRYFQKVAAFPQMTMPGD
                     EVDEMIGIINDAGAGVIGTPDTARAQVQRLWDQSGGFGAMLQMGHEWANPAATKRSAE
                     LFAAEVIPHFQGQA"
     gene            1348..1806
                     /locus_tag="VGP27_07400"
     CDS             1348..1806
                     /locus_tag="VGP27_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7851175.1"
                     /translation="MSDTDEIGLWAFADEGNVYWQYLPIEPLGPRRNEMTNLIQQLIP
                     SGGTPLYAVTRQASEEVGKTARSDAINAIVVMTDGKNENPDDTDLDGLIRQLTHQASE
                     GGIRVFTIAYGQDADLDTLKRISEASRAAAYDATDPQTIDTVFANVLSNF"
     gene            1806..2117
                     /locus_tag="VGP27_07405"
     CDS             1806..2117
                     /locus_tag="VGP27_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851176.1"
                     /translation="MAHNRFRERLLESMRDPWALLLSTFGGATAWAFGASIPLSAATT
                     VVMLGTAAAFGALTRTRHETPAQLRVGTRQQGLINLLDGHVRSLRALHSNAARCRAAQ
                     G"
     gene            2101..2496
                     /locus_tag="VGP27_07410"
     CDS             2101..2496
                     /locus_tag="VGP27_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851177.1"
                     /translation="MQPKADDALAAADAARPSVVQMAAAVDALDEAIAAARNVSGQGE
                     HATESIKNTVDRLTSRRAKLLDRLTAAVDEVATVYAGLLELSATARTMGVSVDDTDVG
                     AVNDSVTLLQMTFAELEADAANLTTEGLV"
     gene            complement(3311..3481)
                     /locus_tag="VGP27_07415"
     CDS             complement(3311..3481)
                     /locus_tag="VGP27_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851178.1"
                     /translation="MAHAGLDDLPGERQAVDDVFCDDGQGFGTKEVAARLLFLHSVKP
                     LLGGCAAKGVMT"
     gene            complement(3714..4199)
                     /locus_tag="VGP27_07420"
     CDS             complement(3714..4199)
                     /locus_tag="VGP27_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEV7851179.1"
                     /translation="MSTGGIVLVALVIAVGLIGIVVPLLPGILLVYAAILVWALVEHN
                     VTSWVTLAVVTVLIGVTTLTKYMWPMKRMRAADVGTLTLLAGAVLGIVGFFVIPVVGL
                     VIGFVLGVYLAELANRHDQRIAWVSTKHALKGVALSVGVELAGALLATIAWVFGVYLS
                     A"
     gene            complement(4247..>4760)
                     /locus_tag="VGP27_07425"
     CDS             complement(4247..>4760)
                     /locus_tag="VGP27_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7851180.1"
                     /translation="AWQLPTEWLEIPAGGVALDIGSGPGNVTAALARAAGSDGLALGI
                     DISEPMLARAVEAQAGRNVGFMRADAQQLPFRDETVDAVTSLAVLQLVPNPSATLAEI
                     ARVLKPGGRVAIMVPTAGRGADFLKWLPNGGVTFFTEDELGDTFEDLGLVGVRTKTLG
                     NIQWVRARKP"
BASE COUNT          909 a         1588 c         1516 g          747 t
ORIGIN      
        1 gcctgaccct ggaaatgtgg aatgacctct gcggcaaaga gttccgccga gcgtttggtg
       61 gcagcggggt tggcccactc gtgacccatc tgcagcatgg cgccgaagcc gcccgactgg
      121 tcccacagcc gctgcacctg agcccgtgcc gtatcgggcg tgccgatcac gccggcaccc
      181 gcgtcgttga tgatgccgat catctcgtcg acctcgtcgc cgggcatcgt catctgcggg
      241 aatgccgcca ccttctggaa gtaccggaac cacgacgcga tgccgaagcg gacgtcgtcg
      301 cgggcctgct cctccgtctc ggcgatgtgc atgggacaga ccagcgacca gttcctgcga
      361 tcgacggtgg tgccgaacgc ggccgcccgc tcctcggcga tgccccagtg gaaagcgagc
      421 gcgtcgaaac cctcgacgat cagcgttgcg ccgatcgaca gcagcccgat gccgtgcttg
      481 cccgccagcc gggcgccggt cggtgaggcc acggccgcaa ccgacagcgg gatgccgccc
      541 tcggagtacg gcgccagctg cagctgggcg tcgtgcagtt cgtgggtcgc cgtcttggcg
      601 gtgacggtct cgcctgccag cagccggacc agaatgtcga ggttggtttc cagtaactcc
      661 cgggtgtcgg tgggggtcaa cccgatcatc gacgagtcgg taggcagcga gcccggcccg
      721 acgccgccga tgatccggcc atgggtgagg tggtcgagca gcatcagccg gtcggcagcc
      781 cacagtgggt tgtggtacgg cagtgagatg acgccggtcc cgaaccggat ccgcttggcc
      841 cgctgcgccg cagcggcgat gaacacctcc ggtgagctga tgatctcgct gcccgccgag
      901 tggtgctcac cgatccacac ctcgtcgaag ccgacccggt cgagatgctc gacgaactcg
      961 aggtcgcgct gcagcgcgag ggtggggttg gtgcccgcgc ggtggaacgg cgcgatgaag
     1021 tatccgaaac ggagcttggc catgcctaga acctagccac gtcgtccgcg gccgatgtgt
     1081 caattgcgct agaggggcat ggttcgctga ttacgctcga tcatctggca gttggcctag
     1141 cttgcacagc gaagaaatcg gcgtccccac actgtccgcg ccgagtccgc aagtgctcgc
     1201 cgcggtgcga gccacctggg gcgaactgcg caagaaagca cacgtcttgc tggtgctcga
     1261 cgtgtcgggg tcaatggaac agtcggccgg ggcgggcaag accaggctgg aactcgcgcg
     1321 tccgcggcgg tgcgggggct ggcccagttg tccgacaccg atgagatcgg tctgtgggcg
     1381 tttgccgacg aaggcaacgt gtactggcag taccttccga tcgaaccgct cgggccgcga
     1441 cgaaacgaga tgaccaacct tatccagcag ctgatcccat cgggcggcac accgctgtat
     1501 gccgtcacgc gccaggcatc cgaggaggtc ggcaagaccg cgcgctcgga cgccataaac
     1561 gccatcgtgg tcatgaccga cggcaagaac gagaatcccg acgacaccga cctcgacggg
     1621 ctgatccgcc aactcaccca ccaagcatcc gagggcggca tccgggtgtt cacgatcgcc
     1681 tacggccaag acgccgacct ggacaccctc aaacgcatct ccgaggcgtc tcgagccgcc
     1741 gcatatgacg ccaccgaccc gcagacgatc gataccgtgt tcgccaacgt cctgtcgaac
     1801 ttttgatggc gcacaatcgc tttcgagagc gcctgctgga gagcatgcgc gacccgtggg
     1861 cactgctgtt gtcgaccttc ggcggcgcaa cggcgtgggc gttcggcgct tccatcccgc
     1921 tgtccgcggc caccacggtc gtgatgctcg ggaccgcggc agcgttcggc gctctcacgc
     1981 gcacgcggca cgagacaccg gcgcagctgc gagtcggcac gcggcagcag ggactgatca
     2041 accttctcga cggtcatgtg cgctcattgc gggcgctgca cagcaacgct gcccgatgtc
     2101 gtgcagccca aggctgacga cgcactggcc gcggctgacg ctgcccggcc ctctgtggtg
     2161 cagatggccg ccgcggtgga cgcgctagac gaggcgatcg cggccgcccg caatgtatcc
     2221 ggccagggcg agcacgcgac agagtcgatc aaaaacacgg tcgaccggtt gacatcgcga
     2281 cgggccaaac tgctcgacag actcaccgcc gcggtagacg aagtagccac cgtctacgcc
     2341 ggactgctcg agctgtcagc gaccgcacgc accatgggcg tctcggtgga cgacaccgac
     2401 gtgggagcgg tcaacgactc agtgaccctg ctgcagatga ccttcgccga actggaagcc
     2461 gacgccgcga acctaacgac cgaaggactc gtctaatcgc cgtcgatgag tccgcccggg
     2521 atgagcgcac gtgaggaaga gctattcggc tacatacacc aaatcttcac ccagcgtcgc
     2581 ggtccaagtg gatcttgaga actcgccacc acgccagaca tgaactcgcg tagggcttct
     2641 caccggagcg tttcaggccg ccaagtgctt ctaagtcacc ttcaccgatt gacaccatcc
     2701 tcgatggtgg ctggcccgca gggttctcga tgtttctcgc agcgggtact actccgaccc
     2761 acgcctcacc tcgaaccagt acggccagtc gtcgctcagc tgtacctgtc ctgacacggg
     2821 gaggagaaac gctgtgcgcc aagcaaaagg agaggacgca aacgaaacaa ggcgatctac
     2881 gtctcccgaa tcccaacgta ctcggtttcc gggagaccaa tcgtgcgctg caatccaagc
     2941 aatcttgggt gccaagccaa gtgcagcctc ggacgcaatt cgaaagaaag tggcgatttc
     3001 gcctacgaga gcaccgggct ctccaccgga gattagcggc gctgcctcga aaattcctat
     3061 gtcggcagga caggcgaaaa ccagtgcatc ctcagccggt gagccgaaca gcgatgcggc
     3121 cccggctcgc acgtagggac gacactgaga cagtccccgc gccaggaaaa ctcccaaatg
     3181 ttcgattgca gtctggacag tttcctcggc gcccacccag tcctcttcac gatgaccgag
     3241 cgggaagagc aacagtgacg ctccagtggt tctgggccaa gccggaggtt gaatcgtcat
     3301 cgtactcaat ttaggtcatt acccctttgg ctgcgcatcc gcccagcaac ggtttcaccg
     3361 aatgcagaaa taacaaccgg gcggcgacct ccttggtgcc gaagccttgg ccgtcgtcgc
     3421 agaagacgtc gtcgaccgct tgacgttcgc cgggcagatc atcgagaccg gcatgcgcca
     3481 cgatgagttg ggagaacctc ggccaacacg cggtccaacg ccgcaacagc ggccgtcatc
     3541 gcctcgggct ggctttccca cagctgcgtg gaaccttcga cgtcggccag cagcaatgtc
     3601 actgttcctg tcggcagcaa atcgctcacg actacatcgc tccggctcaa cggcttgact
     3661 ggcctggtca gatccaaccg ttcaatgcta actagaaggc ggccgagaag tggctatgcc
     3721 gatagataca ccccgaacac ccaggcgatg gtggctagca gcgcgccggc caactcgacg
     3781 ccgaccgaga gggccacgcc cttgagcgcg tgcttcgtcg agacccaggc gatccgctgg
     3841 tcatggcggt tggccagctc agccaggtac acgcccagca cgaacccgat caccaggccg
     3901 acgacgggga tcacgaagaa gccgacgatg ccgaggaccg cgccggccag cagtgtgagc
     3961 gtgccgacgt cggcggcgcg catgcgcttc atcggccaca tgtacttggt cagcgtggtc
     4021 acgccgatca gcacggtcac caccgcgagc gtcacccacg acgtgacgtt gtgttcgacc
     4081 agggcccaca ccaggatcgc cgcgtacacc agcaggatgc cgggcagcag cggaacgacg
     4141 atgccgatca gcccgacggc gatgacaagt gcgacaagga cgatcccgcc ggtgctcacg
     4201 gctcgaacct actgcggacg tgaggagcac caaccgacaa cagtcgtcag ggctttctcg
     4261 cacgcaccca ttggatgttg ccgagcgtct tcgtgcggac accgaccagg ccgagatcct
     4321 cgaaggtgtc gccgagttcg tcctcggtga agaaggtgac cccgccgttg ggcagccact
     4381 tgaggaagtc ggcgccgcgg ccggcggtcg gcaccatgat ggctacccgt ccgccgggct
     4441 tcagcacccg cgcgatctcc gccagggtgg ccgacgggtt gggcaccaac tgcagcacgg
     4501 caagcgaggt gaccgcgtcg acggtctcgt cgcggaacgg cagctgttgc gcgtcggcgc
     4561 gcatgaagcc gacgttgcga cccgcttggg cctcgacagc acgcgccagc atcggctcgg
     4621 agatgtcgat gcccaacgcc agcccgtcgg aacccgcggc gcgggccagg gcggccgtga
     4681 cgttgcccgg gccgctgccg atgtcgagcg cgacgccacc ggccgggatc tccagccact
     4741 cggtgggcag ctgccacgcg
//
LOCUS       DASYLX010000304         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2865514,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000304 DASYLX010000000
VERSION     DASYLX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865514"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGP27_07430"
     CDS             complement(<1..776)
                     /locus_tag="VGP27_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851181.1"
                     /translation="MRVGRKETVAVIMAVLLVVAAFVVPHLHLGIVTPLIHATPQRFR
                     DFAGTAPIFGWWNAHVGWGTAPAILIGAATVLWGPTIAQRLPWRALTFATWATSCLWA
                     FSLAMIDGWQRGFAGRLTARHEYLRQVPTITDIPEALSSFSRRILDYQPDSWITHVSG
                     HPPGALLTFVWLDRIGLSGGAWAALLCLLVGSSAAAAIVVAVRAVADEETARLVAPFV
                     AVAPTAVWVAVSADGYFAGVAAWGIALLALAVKQKVRVPLL"
     gene            950..1537
                     /locus_tag="VGP27_07435"
     CDS             950..1537
                     /locus_tag="VGP27_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851182.1"
                     /translation="MARPRKFDEGDVVAAVRDEFWNRGYAATSVDDLSAATGLGKGSL
                     YGAFGDKHSLYLRALDDYIASSLDDVRVSLRDPRYSAYDRLSRHIRGQVKAIVADTRR
                     RGCLMAKSAAELGADDTVESRVQRAYSVWRDELAACIKEAQRDGAIAAEQNPRALAST
                     LLAFLRGQEALHKGGARPAQLKAAADEMVSLIPTG"
     gene            1612..>2232
                     /locus_tag="VGP27_07440"
     CDS             1612..>2232
                     /locus_tag="VGP27_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851183.1"
                     /translation="MGLLTGKTAVITGGSTGIGFATAKRFVDEGAYVFITGRRQAELE
                     AAVKELGDNATGVQGDVSRREDVDRLYQVVADNSRRIDVVFANAAILEVARVGEITEE
                     QLDYQLGVDFKGVVFTVQQALPLFNDGGSIILNASTTAGRAADGISVYGAIKAGIRSL
                     ARTWASELRDRKIRVNAVSPGATDTPGIDTLARMLFSGPNAEEQFEE"
BASE COUNT          422 a          732 c          767 g          311 t
ORIGIN      
        1 agcagaggca cccgcacctt ctgcttcacc gccagtgcca acagcgcgat gccccacgcc
       61 gcgacccccg cgaagtaacc gtcggccgac acggcaaccc agacggccgt cggtgcgacc
      121 gcaacgaacg gcgcgacgag ccgggcggtc tcttcatcgg cgacggcgcg caccgcgacg
      181 acgatcgcgg cggctgcgct ggatccgacg agcaggcaca acagggccgc ccatgcaccg
      241 ccgctcaggc cgatgcgatc caaccagacg aaggtcagca aggcccccgg tggatggccg
      301 gacacatggg tgatccaaga atcgggctga tagtccagga tccggcggga gaagctgctg
      361 agcgcctcgg ggatgtcggt gatcgtcggc acctggcgga ggtactcgtg gcgggcggtc
      421 agccggcctg cgaaaccgcg ttgccagccg tcgatcatgg cgagggagaa cgcccacagg
      481 cacgacgtcg cccacgtcgc aaacgtcagc gcgcgccacg gcagccgctg cgcgatggtc
      541 ggtccccaca gcaccgtcgc cgcgccgatg aggatggcgg gcgcggtgcc ccacccgacg
      601 tgggcattcc accatccgaa gatcggggcc gtgccggcga agtcgcggaa acgttggggc
      661 gtggcgtgga tcagcggcgt cacgatgccg aggtgcagat gcgggaccac gaacgccgcc
      721 accaccagca ggacggccat gatcaccgcc accgtctctt tccggccgac ccgcattcac
      781 gccagcgtag gggcaggcgg gcaaatacgg tcgccctgcc ggctatgggt ggaaggcgag
      841 gcggtcagac cgctcggccg gcggcgcgac tggccgacgt ggcagaagag ggaggcgggg
      901 tggtcaagga tacggaacaa tcggtccata acttcggtac tgtgtagcca tggcgcgtcc
      961 ccgcaagttc gacgaaggtg acgtcgtcgc cgccgtgcgc gacgagttct ggaaccgcgg
     1021 atacgccgcg acgtcggtcg atgacctctc ggccgcaacc ggattgggca aaggcagcct
     1081 ctacggcgcc ttcggcgaca agcactccct ttacctgcgt gcgctcgatg actacatcgc
     1141 gtcatcgctc gacgacgtcc gcgtcagcct gcgcgacccg cggtacagcg cctacgaccg
     1201 gctttcgcgt catatccgcg ggcaggtcaa ggccatcgtc gcagacaccc gcaggcgcgg
     1261 ctgcctgatg gccaagagcg ccgcggagct cggtgccgac gacaccgtcg agtcgagggt
     1321 gcagcgggcc tattcagtct ggcgggacga acttgccgcg tgcatcaagg aagcccagcg
     1381 agacggggcg atcgccgccg agcagaaccc tcgcgcgctg gcgagcacgc tgttggcctt
     1441 cttgcggggc caggaagccc tgcacaaggg cggggccagg cccgcacagc tgaaggcggc
     1501 cgccgacgaa atggtgtctc tgattcccac gggctagcgc ggaatagatc cgcggttgcg
     1561 agtgtctcta gtttttgacc gaatggtaca gaactagagg aagacaccac aatgggactt
     1621 ctcacgggaa agacggcagt catcaccgga ggcagcaccg gaatcggctt cgccaccgcc
     1681 aaacgattcg tcgacgaagg cgcatacgtg ttcatcaccg gacgccgaca ggccgaactc
     1741 gaggcggcgg tgaaggaact cggtgacaac gccaccggcg tacagggcga cgtgagtagg
     1801 cgcgaagacg tcgaccggct gtatcaggtc gtcgccgaca acagtcgtcg catcgacgtc
     1861 gtgttcgcca acgcggcgat cctcgaggtt gcccgcgtcg gcgagatcac cgaggagcag
     1921 ctggactatc agctgggcgt cgacttcaag ggcgtcgtgt tcacggtgca gcaggccctc
     1981 ccgctgttca acgacggcgg atcgatcatc ctgaatgcgt ccaccaccgc cggtagggct
     2041 gccgacggaa tcagtgtcta cggggcgatc aaggcgggca tccgctctct tgcccgcacc
     2101 tgggcgagtg agctgcggga ccgcaagatt cgcgtcaatg cggtgagccc gggcgccacg
     2161 gacacgccag ggatcgacac gctcgcgcgc atgctgttct cggggcccaa tgccgaggag
     2221 cagttcgagg ag
//
LOCUS       DASYLX010000305         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2535378,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000305 DASYLX010000000
VERSION     DASYLX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535378"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VGP27_07445"
     CDS             complement(<1..854)
                     /locus_tag="VGP27_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7851184.1"
                     /translation="MVSKASAFKTPNNEPTVRLTVAQAVVRFLANQYVERDGERSKFF
                     AGCFGIFGHGNVAGMGQALLQAEVEAEQAGTEPGLRYVLGRNEQAMVHTAAAYARQKD
                     RLQAWVVTASIGPGSTNMLTGAALATINRLPVLLLPSDTFATRVSAPVLQDLEQPHDN
                     EMTVNDAFKPLSRFFDRVWRPEQLPSALLGAMRVLTDPVETGTATIALPQDVQAEAFD
                     WPETLFAERTWHVARPLPERSMVAQAAEIIRSAERPLIVAGGGVIYSGANDALATFVE
                     RTGIPVSET"
     gene            complement(864..>1655)
                     /gene="iolB"
                     /locus_tag="VGP27_07450"
     CDS             complement(864..>1655)
                     /gene="iolB"
                     /locus_tag="VGP27_07450"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEV7851185.1"
                     /translation="WSEAGLWVVELAPQQALTLDTNGIEVMILPLAGGGSVECGDELF
                     QLAPRATVFAGPADMAYIGTNQSYTLRGEGRIAICAARAKRALPNRKVAAADVPVELR
                     GTGNCSRQVHNFGTAGVFEADSLIACEVITPGGNWSSYPAHKHDEESPVESALEEIYY
                     FEIASGPDDSRGFGYHRVYGTPSRPIEVLEEVRTGDVVLVPHGYHGPSVAAPGYHMYY
                     LNVMAGSGEERAWKIVDDPEHAWLRDTWEDQAVDPRLPLHANPSV"
BASE COUNT          257 a          568 c          551 g          279 t
ORIGIN      
        1 gtctccgaca ccgggatgcc ggtgcgctcg acgaacgtcg ccagcgcgtc gttggcgccg
       61 gagtagatga cgccgccgcc tgccacgatg agcgggcgct ccgccgaccg gatgatctcg
      121 gcggcctgcg cgaccatcga ccgttccggc agcgggcgtg ccacgtgcca agtgcgttcg
      181 gcgaacagcg tttccggcca gtcgaacgcc tcggcctgca cgtcttgggg cagcgcgatg
      241 gtcgctgtgc ccgtctcgac cgggtcggtg agcacccgca tcgcacccag cagcgccgac
      301 ggcaactgct cgggccgcca cacccggtcg aaaaaccgcg atagcggctt gaaggcgtcg
      361 ttgacggtca tctcgttgtc gtgcggctgc tcgaggtctt gcagcacggg tgcgctcacc
      421 cgggtggcga aggtgtcgga cggcagcagc agaacgggca gccggttgat ggtggccagc
      481 gccgcgccgg tcagcatatt cgtggagccc ggcccgatgc tcgcggtcac cacccacgcc
      541 tgcaggcgat ccttctgcct ggcgtacgcc gcggcggtgt gcaccatcgc ttgctcgttg
      601 cgccccaaca catatcgcag gcccggctcg gtcccggcct gctcggcttc gacctcggcc
      661 tgcagcagcg cctgacccat gcctgccacg ttgccgtgtc cgaagatgcc gaagcagcct
      721 gcgaagaact tgctgcgttc gccgtcgcgc tcgacgtact ggttggccag gaagcggacg
      781 acggcctggg ccacggtgag ccgcaccgtc ggctcgttgt tcggtgtctt gaacgcggat
      841 gctttggaga ccacgtcaca ctcctataca gaagggttgg catgcagggg cagccgggga
      901 tcgacggcct ggtcctccca ggtgtcgcgc aaccaggcgt gctcggggtc atcgacgatc
      961 ttccaggccc gttcctcgcc ggagccggcc atcacattga ggtagtacat gtggtagccg
     1021 ggtgctgcga ccgacgggcc gtggtagccg tgcggaacca gcacgacatc accggtgcgc
     1081 acctcttcga gaacttcgat gggcctgctc ggcgtgccgt acacgcggtg ataaccgaaa
     1141 ccacgcgaat catccgggcc ggaagcaatc tcgaagtaat agatctcctc gagcgccgac
     1201 tcgaccggcg actcttcgtc gtgcttgtga gcgggatagc tcgaccagtt gccgccgggg
     1261 gtgatcacct cgcaggcgat cagcgagtcg gcctcgaaga cgcctgcggt gccgaagttg
     1321 tgcacctgcc tgctgcagtt gccggtaccg cgcagctcga ccggcacgtc cgcggcggcc
     1381 actttgcggt tggggagcgc acgcttggcc ctggcggcgc agatcgcgat cctgccctcg
     1441 ccgcgcagcg tgtagctctg gttggtgccg atgtaggcca tatccgcggg gccggcgaaa
     1501 accgtggccc gcggggccaa ttggaacaac tcgtctccgc attcgacgct gccgccaccc
     1561 gccagcggca ggatcatcac ctcaatgccg ttcgtgtcca acgtcagcgc ctgctgaggc
     1621 gccagctcca ccacccacag gccggcctcg gacca
//
LOCUS       DASYLX010000306         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1768176,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000306 DASYLX010000000
VERSION     DASYLX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768176"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="VGP27_07455"
     CDS             <1..>1629
                     /locus_tag="VGP27_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7851186.1"
                     /translation="AADALGPGALGPVRVLVTFPDGNSASAPTKEPLLQRVQQRMSQA
                     PNVVTVQPAVFGNDYRSALVSAVLTVDPEDMAARQSVDWMRAELPPVAGNEATIDVGG
                     PTALIKDFDDRVSKTQPAVFGFVALIAFVMLLVSIRSVFLAFKGVLMTVLSVAAAYGS
                     LVAVFQWGWLEALGFRPIASLDSTIPPLVLAMTFGLSMDYEIFLLTRIRERFLQTNNT
                     RDAVAYGVSTSARTITSAALIMIAVFIGFAFAGMPLVAQLGVACAVAIAVDATVVRLI
                     LVPALMAMFDQWNWWLPRWLDRLLPSVDFEKPLPKADIGDLVIIPDDISTLAASGIDI
                     RNVVKSAAKLKNMAPHTISVADPLAFSGCLPSNGSDCIKALNGHTNGHSHTNGAGNGK
                     TVKLKRGPRPMHPVTMWRGRLSVALDALETEAETDRPAVERNSPMETTNVQLPTGDRL
                     SIPTGAETLRLKSYLIMCRNSTKDFSEFADLVAHMETQTAAVVLAGMDRYYCGEPSRK
                     QWVATQLVRRLADPHPSDEFDIPMSGAEAEADWAK"
BASE COUNT          266 a          536 c          542 g          285 t
ORIGIN      
        1 gcggcggacg cgctcggccc cggcgcgctc ggtccggtcc gcgtgctggt gacgttcccc
       61 gacggcaact ccgcctccgc gccgacgaag gaacctcttc tgcagagggt ccaacagcga
      121 atgtcgcagg cgcccaacgt cgtcacggtg cagccggcgg tgttcggcaa cgactaccgc
      181 agcgcgctgg tttcggcggt gctgacggtg gacccggagg acatggccgc ccgccagtcc
      241 gtcgactgga tgcgcgcgga gctgccaccg gtggcgggca acgaggcaac gatcgacgtc
      301 ggcggaccaa ccgcgttgat caaggacttc gacgaccggg tgagcaaaac acagcccgcg
      361 gtgttcgggt tcgtcgcgct gatcgcattc gtgatgctgc tggtctccat ccgatcggtg
      421 ttcctggcgt tcaagggcgt gctgatgacg gtgctgtcgg tggccgccgc ctacggcagc
      481 ctggtcgccg tcttccagtg gggctggctc gaagcgctcg gtttccggcc catcgcgtcg
      541 ttggacagca ccattccacc gcttgtgctg gcgatgacgt tcgggttgtc gatggactac
      601 gagatcttcc tgctcacccg aatccgcgag cggttcctgc agacgaacaa cacccgcgac
      661 gccgtcgcct acggcgtgag caccagcgcg cgcaccatca ccagcgcggc gctgatcatg
      721 atcgccgtct tcatcggctt cgcgttcgcg ggtatgccgc ttgtcgccca gttgggtgtc
      781 gcgtgcgcgg tggcgatcgc cgtcgacgcg accgtcgtgc ggttgatcct ggtgcccgcg
      841 ctgatggcga tgttcgacca atggaactgg tggctgccgc gttggctgga ccgtctgctg
      901 ccgtcggtgg acttcgagaa gccgcttccc aaggccgaca tcggcgatct ggtcatcatt
      961 ccggacgaca tctcgacgct ggcggcatcg ggcatcgaca tccgcaacgt ggtcaagtcg
     1021 gcggccaagc tgaagaacat ggctccgcac acgatctccg tcgccgatcc tctcgcgttc
     1081 agcggctgcc tgccctccaa cgggtcggat tgcatcaagg cactcaacgg ccacaccaac
     1141 ggtcattcgc acaccaacgg cgccggcaac ggcaagacgg tcaagctgaa gcgcggaccg
     1201 cggcccatgc atcccgtgac gatgtggcgc ggccggctgt cggtggcgct ggacgcgctg
     1261 gagaccgagg ccgaaaccga tcggccggcc gtcgagcgca acagcccgat ggagaccacc
     1321 aacgttcagc tgcccaccgg cgaccggctc tcgataccga ccggcgccga gacgctgcgg
     1381 ctcaagagct acctgatcat gtgccggaac agcaccaaag acttctctga gtttgccgat
     1441 ttggtcgccc acatggagac tcaaaccgcc gctgtggtgc tggccgggat ggatcggtac
     1501 tactgtggtg agccatccag gaagcaatgg gtggccacgc agctggtgcg ccggttggcc
     1561 gatccgcatc cgtcggatga gttcgacatt ccgatgtcgg gcgccgaggc ggaggctgat
     1621 tgggccaag
//
LOCUS       DASYLX010000307         5147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1877641,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000307 DASYLX010000000
VERSION     DASYLX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877641"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGP27_07460"
     CDS             complement(<1..923)
                     /locus_tag="VGP27_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7851187.1"
                     /translation="MDTSDVLVVGAGPTGLMLAAELALGGVGVRLLEERIDMPNITRA
                     FAVHARTLELLDARGLADELLKRGKLVYQIAPPGGASIDLRALPSRFGVLLIAPQSGT
                     EHVLAARVDELAVPVSRGAELVGLTQDDDGVTVQCADGTAMRAAYVVGCDGAHSTVRQ
                     LVGIGFEGKQYATHILLADVRLTRAPEETLTGVTNERGVVLMIPFGDGWFRAIAWDRL
                     REQAPLKEPVTLEEIRGSFNRVADEDFGMTEMRWSSRFLSERKQAEHYREGRVFIAGD
                     AAHVHSPLGGQGMNTGIGDAMNLGWKLSSAVR"
     gene            1053..3935
                     /locus_tag="VGP27_07465"
     CDS             1053..3935
                     /locus_tag="VGP27_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HEV7851188.1"
                     /translation="MLAVLIAHAVATALAPLLVRRWGRLAFYPLALVPLGSLVWVTQN
                     WPGHGRALRVDVPWVPELSMNIALRFDTLAAIMSVLVLGIGALVLFYCADYFHHHDGH
                     TENRLPSFAAELVAFAGAMFGLVISDNMLVLYVFWELTTVLSFLLVGHYAERVSSRKA
                     AVQALLVTTAGGLAMLVGIVILGTISGTYLLSELIAAPPSGLAASVAIVLVLVGALAK
                     SAIVPLHFWLPGAMAAPTPVSAYLHAAAMVKAGVYLVARLTPGFADSPPWRPVVVTLG
                     VLTVLLAGWRAVREYDLKLILAFGTVSQLGLITVMVGAGGGDLMLAGLALLCAHAMFK
                     AALFMVVGIIDHATGTRDIRRLAWLGARSKPLLVIAAGATASMAALPPFLGFVAKEAD
                     YETLLHSESLGHAAPFVLAAIVAGSVFTTIYSLRFLWGAFGKKGRPEPSKRVAEMYPV
                     PATFLAPSAILAAAGLLFGLWPSPLEEALDPYADTVPGGADYELALWHGFNLPLLLSA
                     LVLAVGTVVFFTRSRLPRTRLAYQPLGNADRIYDAVIRGADVLSVRLTGTTQRGSIPA
                     TQSVILFTLAVVPVVALTLGARDRPELALWGSPLQVIVGLLTLVAAVGATVMRNRLAA
                     VLLVGVTGYGCGTIFAFHGAPDLALTQFLVETLTLVIFVLVLRTLPAESDHGNIRRHR
                     LPRAALALAVGASVTTLAVFAMAARTGVPIAALLPDAAYYRGHGANTVNVLLVDIRAW
                     DTLGEISVLLVAATGVASMVFRHRRFGAAPRVADAGLPDIAPPAAIATSPAAGDITWL
                     RGSELRDPRNRSLVLEVATRMIFPLIMVLSAYFFFAGHNTPGGGFAGGLTAGLALVLR
                     YLAGGRYELGEALPLDAGKILGVGLALSAGTAVASLLLGAPVLSSAVLQFDIPVLGTV
                     KLVTALFFDLGVYLIVVGLVLDVLRSLGARVDEDLAERRSAAVTAR"
     gene            3932..4402
                     /locus_tag="VGP27_07470"
     CDS             3932..4402
                     /locus_tag="VGP27_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HEV7851189.1"
                     /translation="MTAFLVPLILVGGLTSVGVYLLLERNLTRMLLGLLLIGNAVNLL
                     ILLVGGPSGNPPVRGRISDGNTTTADPLAQGMILTAIVISMGIAAFVLSLIYRTYRLT
                     TEEEVANDPEDTRVSQMSDEEATAFDEGRPEPDTARDTDEPDELDALPGLEGSR"
     gene            4399..>5147
                     /locus_tag="VGP27_07475"
     CDS             4399..>5147
                     /locus_tag="VGP27_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV7851190.1"
                     /translation="MSISGVLTPLPVLIPLLGAAATMFAGRRPRLQRAITLAALTAVV
                     AVCAALLYLTDRDGTLVAHVGGWSQSVPGMGPLGITLVVDRLSALMMVVSSIVLLAVV
                     FYAIGQGIRDGDERQPVSIFLPTYLALSAGVCNAFLAGDLFNLFVGFEVLLAASFVLL
                     TIGASTERVRVGISYVMVSMVSSMIFLIGIALVYAATGTLNMAELATRLDGVASGTRS
                     ALFAVLLVAFGIKAAVFPLSTWLPDSYPTAPA"
BASE COUNT          697 a         1817 c         1697 g          936 t
ORIGIN      
        1 cggacggccg acgacagctt ccagccgagg ttcatcgcgt cgccgatgcc ggtgttcatc
       61 ccctgtccgc ccagcggcga gtgcacatga gcggcgtcac ccgcgatgaa cacccggcct
      121 tcgcggtagt gctcggcctg cttgcgctcc gacaaaaacc tcgaactcca acgcatttcg
      181 gtcatgccga agtcttcatc ggcgacgcgg ttgaacgagc cgcgaatctc ctccagcgtc
      241 accggctctt tcagcggcgc ctgctcacgc agccggtccc aggcgatggc acgaaaccag
      301 ccgtcgccaa acggaatcat cagcaccacg ccccgctcgt tcgtgacacc ggtcaacgtc
      361 tcctccgggg cccgcgtcag ccgcacgtcg gccagcagga tgtgcgtcgc gtactgcttg
      421 ccttcgaacc cgatgccgac caactgccga accgtgctgt gcgcgccgtc gcaacccacc
      481 acgtacgccg cccgcatggc ggtgccgtcg gcacactgca ccgtcacgcc gtcgtcgtcc
      541 tgggtcaacc cgaccagctc ggcgccacgc gaaaccggca ccgccagctc gtcgacccgg
      601 gccgccagca catgctcggt gccgctctgc ggcgcgatca acagcacccc gaaccggctc
      661 gggagcgccc gcaggtcgat cgaggcgccg cccggcggag ctatctggta aacgagtttg
      721 ccccgcttca gcagttcgtc ggccagcccg cgcgcgtcca gtagctccag cgtgcgcgca
      781 tggacggcga acgccctggt gatgttgggc atgtcgatgc gctcttcgag caaccgaacc
      841 ccgacgccgc cgagagcgag ttcggcggcc agcatcaaac cggttgggcc ggccccgacc
      901 actagcacat cggaggtatc cacgggccgg acggtacctg cattcgccca cgtagactgg
      961 gcttcagcca aagcctcacc cggctgacac cctgctatct gttcttcgcc cggcggctca
     1021 tcaccgacca agcccaccag attcggagtg ccttgctagc cgtcctgatc gcccacgcgg
     1081 tcgccaccgc cttggctccc ctgctggtgc gccggtgggg caggctggcg ttctatccgt
     1141 tggcgctggt gccgctcggc tcccttgtct gggtgacgca gaactggccg ggacacgggc
     1201 gggccctgcg cgtcgacgtg ccgtgggtgc ccgagttgtc gatgaacatc gcgctgaggt
     1261 tcgacacgct cgcggcgatc atgagcgtgc tggtgctggg catcggcgcg ctggtgctgt
     1321 tctactgcgc cgactacttc catcatcacg atgggcatac cgagaaccgg ctgcccagct
     1381 tcgctgcgga gctcgtcgca ttcgcgggcg ccatgttcgg gttggtgatc agcgacaaca
     1441 tgctggtgct ctacgtgttc tgggagctga ccaccgtgct gtccttcctg ctggtcggtc
     1501 actacgccga gcgcgtcagc agtcgcaaag ccgccgtgca ggcgctcctc gtcaccacgg
     1561 caggcggcct ggccatgctg gtcggcattg tcatcctcgg caccatctcg ggcacctacc
     1621 tattgtccga gctgatagcc gcgccgccgt ccgggctggc ggcatccgtc gccatcgtcc
     1681 tggttcttgt tggcgcactt gccaagtcgg ccatcgttcc gttgcatttc tggctgccgg
     1741 gcgcaatggc cgcccctacg ccggtcagcg cctacctaca cgccgccgcc atggtcaagg
     1801 ccggtgtcta cctggtcgca cgcctgacgc cgggcttcgc cgactcgccg ccgtggcggc
     1861 cggtggtggt caccctcggc gtgctgacgg tgctgctcgc gggctggcgc gcggtgcgcg
     1921 aatacgacct caagctgatt ttggcgttcg gcacagtgag ccaactcgga ctcatcaccg
     1981 tgatggtcgg cgcaggcggc ggcgacctga tgctggccgg gctggcgctg ctgtgcgcgc
     2041 acgccatgtt caaggccgca ctgttcatgg tggtcggcat catcgaccat gcgaccggca
     2101 cccgcgacat ccgcaggctg gcctggctcg gagcgcgcag taagccgctt ctcgtcatcg
     2161 ccgccggcgc caccgcgagt atggccgcgc tgccgccgtt cctcggcttc gtcgccaaag
     2221 aggccgacta cgaaaccctg ctacacagtg agtcattggg acatgccgcg ccgttcgtgc
     2281 tggcggccat cgtcgcgggc tcggtgttca ccaccatcta cagcctccgc ttcctctggg
     2341 gcgccttcgg caagaagggc aggcccgaac ccagcaagcg agtggccgag atgtatcccg
     2401 tgcccgccac attcctcgct ccttcggcga ttctggcggc ggcaggcctg ctcttcgggc
     2461 tgtggccgag cccgctcgaa gaggcgctgg acccctacgc cgacacagtt cccggcggcg
     2521 ccgactacga actggcgctc tggcacggat tcaatctgcc cctgcttctt tcggcgctcg
     2581 tgttggcggt cggcaccgtc gtgttcttca cgcggtcgcg gttgccgcgg acgaggttgg
     2641 cgtatcaacc gctgggcaac gccgaccgca tctacgacgc cgtgatccgc ggcgccgacg
     2701 tgctttcggt ccggctgacc ggcaccacgc agcgggggtc gatacccgcg acccagtcgg
     2761 tgatcctgtt cacgctcgcg gttgtgccgg tggtggcgct gacgctcggg gcacgcgacc
     2821 ggcccgagct ggcgttgtgg gggtcgcctt tgcaggtgat cgtcgggctg ctgactctgg
     2881 tggccgccgt gggtgcgacg gtgatgcgca accggctggc ggcggtgctg ctggtcggcg
     2941 tgaccggcta cggctgcggc accatcttcg cgttccacgg tgcacccgac ctggcgttga
     3001 cccagtttct ggtggagacg ctgacgctgg tcatcttcgt gctggtgctg cggacgctgc
     3061 ccgccgagtc cgaccacggc aacatccgca ggcacaggct gccgagggcg gcgctcgcgc
     3121 tggccgtcgg tgccagcgtc accacactgg cggtgttcgc gatggccgcc cgcaccggcg
     3181 tcccgatcgc cgcgctgctg cccgacgccg cctactaccg cggacacggt gccaacaccg
     3241 tcaacgtgct gctggtcgac atccgcgcgt gggacaccct cggcgagatc tcggtgctgc
     3301 tggtggccgc gaccggggtg gcctcaatgg tgttccggca caggcgcttc ggtgccgcac
     3361 cgcgcgtcgc cgacgcaggc ctgcccgaca tcgcgccgcc tgcggcgatc gcgacaagcc
     3421 cggcggcggg cgacatcacc tggttgcgcg gcagcgagct gcgcgatccg cggaaccggt
     3481 cgctggtgct cgaggtcgcg acgcggatga tcttcccact catcatggtg ctgtcggcgt
     3541 acttcttctt cgcaggccac aacacacccg gcggcggctt cgcgggcggg ctgaccgcag
     3601 gcctcgcgct ggtgctgcgg tatctcgcgg gtggccgata cgaactcggc gaggcgctgc
     3661 cgctggacgc aggcaagatc ctgggcgtcg ggctggccct ttcggcgggc accgcggtgg
     3721 cctcgctgct gctcggtgcg ccagtgctgt cgtcggcggt gctgcagttc gacattccgg
     3781 ttctcggcac cgtcaaactc gtcaccgcgc tgttcttcga cctcggcgta tatctgatcg
     3841 tcgtcggcct cgttctcgac gtgctgcgca gcctcggcgc gcgggtagac gaagacctgg
     3901 ccgaacggcg atccgcggcg gtgacggctc gatgaccgcc tttctcgttc cgctgatact
     3961 ggtcggtgga ctcaccagcg tcggggtcta cctgctgctg gaacgcaacc tcacccgaat
     4021 gctgttgggc ctcttgctca tcggcaacgc cgtcaacctg ttgatccttc ttgtcggcgg
     4081 cccgtcgggt aatccgccgg tgcgtggccg catcagcgac ggcaacacca ccacggcaga
     4141 cccgctggcg caggggatga tcctcaccgc gatcgtcatc agcatgggca tcgcggcgtt
     4201 cgtgctgtcg ctgatctacc ggacctaccg gctgacgacc gaggaggaag tcgccaacga
     4261 cccggaggac acccgcgtct cgcagatgtc tgacgaggag gccacggctt tcgacgaagg
     4321 ccgccccgag ccggacacgg cgcgcgacac cgacgaaccc gacgagctgg acgcattgcc
     4381 ggggctggag ggctcacgat gagcatctcg ggggtgttga ccccgctgcc cgtgctgatc
     4441 cccctgctcg gtgcggcggc gaccatgttc gccgggcgca ggccacggct gcagcgcgcc
     4501 atcaccctcg ccgcgctgac ggcggtcgtc gcggtgtgcg cggcgctgct ctacctgacc
     4561 gaccgcgacg gcacgctcgt agcgcacgtc ggcggctggt cgcagagtgt gcccggcatg
     4621 ggtccgctcg ggatcacgct tgtggtggac cggctttcgg cgctgatgat ggtcgtctcg
     4681 tcgatcgtcc tgctggccgt ggtgttctac gccatcgggc agggcatcag ggacggcgac
     4741 gaacgccaac cggtgtccat cttcctgccc acctatctcg cgctgtccgc gggcgtgtgc
     4801 aatgcgttcc tggcaggcga ccttttcaac ttgttcgtcg gcttcgaggt gctgctggcg
     4861 gccagcttcg tgctgctgac gatcggcgcc agcaccgaac gtgtgcgcgt cggcatctct
     4921 tatgtgatgg tgtcgatggt gtcgtcgatg atcttcctga tcggcatcgc actggtgtac
     4981 gcggccaccg gcacgctcaa catggccgag ctggccaccc ggctggacgg ggtcgcctcg
     5041 ggtacccgca gcgcgctgtt cgcggtgctg ctcgtcgcgt tcggcatcaa ggcggcggtg
     5101 ttcccgctgt cgacgtggct gcccgactcc tatcccaccg cgcccgc
//
LOCUS       DASYLX010000308         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2974554,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000308 DASYLX010000000
VERSION     DASYLX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974554"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGP27_07480"
     CDS             <1..191
                     /locus_tag="VGP27_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEV7851191.1"
                     /translation="GRLVLDAVTNAGEVSAAECAERVGISRVSARRYLEHYLGTGALE
                     IRLQYGVGRPERRYRVVN"
     gene            complement(173..799)
                     /locus_tag="VGP27_07485"
                     /pseudo
     CDS             complement(173..799)
                     /locus_tag="VGP27_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(892..1056)
                     /locus_tag="VGP27_07490"
     CDS             complement(892..1056)
                     /locus_tag="VGP27_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7851192.1"
                     /translation="MTPTEHDVVRLVAEGLGNKDIASRLFVSYRTVQTHPTHVYSKLG
                     MTSRVALLAQ"
     gene            complement(1071..1283)
                     /locus_tag="VGP27_07495"
                     /pseudo
     CDS             complement(1071..1283)
                     /locus_tag="VGP27_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401912.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..1533
                     /locus_tag="VGP27_07500"
     CDS             1303..1533
                     /locus_tag="VGP27_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851193.1"
                     /translation="MRGRVLALLVVAIGGTTPIGAPLMGWLAELFGVRATFMLAGVLT
                     AIAAALAYVYLRRTADEPDDSSVHHSPLSRAR"
     gene            complement(1503..3047)
                     /locus_tag="VGP27_07505"
     CDS             complement(1503..3047)
                     /locus_tag="VGP27_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7851194.1"
                     /translation="MSQLADWFLTAAERGNPASRIPAWCEGSRAEPLIHGATYFDRLV
                     SEVEMLGEGDYLFFTDWRGDPDEKMRDDGPTIRELFCSAAERGVVVKGLVWRSHLDKF
                     AYSEEENQHLGEAIERAGGEVLLDQRVRFGGSHHQKLVVIRHRDEPERDVAFAGGIDL
                     CHSRRDDETHRGDRQAQQMSKEYGDHPPWHDVQLALRGPVVGALDTTFRERWNDPAAL
                     DMLSPIAWLADKLRGADLTPGALPDQPADPPPCGPHSVQVLRTYPDAHFEYDFAPHGE
                     RSIARGYSKAVRRARRLIYTEDQYLWSKRVADLFARALNDNPELHLVAVVPRYPDVDG
                     RLSLPPNLVGRQQAIEACRAADPERVHIFDVENQWGTPVYVHAKVCVIDDVWATVGSD
                     NLNRRSWTHDSELSCAVLDDTGEFARDLRLRLIREHLDRASDGSDDDGLVDLDTVVTT
                     IEATAQALEMWHVNGRTGPRPPGRLRPHRPEKLGALTRSWAEPAYRAFYDPDGRSYRA
                     RLKGEW"
     gene            3119..>3499
                     /locus_tag="VGP27_07510"
     CDS             3119..>3499
                     /locus_tag="VGP27_07510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7851195.1"
                     /translation="MPAVRRALALACALLVLAGCAPTTPPEKESPAPTTDPAKADAVM
                     TIVRDTMAQKHLKAVLVRVTVDGKEVVTRAVGDSMTNVPASTDMHFRNGAVAISYVST
                     LLLILVDEKKVSLDDKLSRWLPDVP"
BASE COUNT          547 a         1186 c         1153 g          613 t
ORIGIN      
        1 ccggtcgcct ggtgctcgat gccgtcacga acgccggtga ggtttccgcg gcggaatgcg
       61 cggagcgcgt cggtatctca cgagtcagcg cgcgtcgcta tctcgagcac tacctcggta
      121 ccggcgcact ggagatccgg ctgcagtacg gcgttggtcg gccagagcgg cgctaccgcg
      181 tggtgaactg acacctacgc ggcatcgtgg cgacgatagc gacaccgatg cgcgtctcga
      241 tctcgctgac ttcaataaga atcggccccg gtaccggtgg tgcagtcgat cgatagtggt
      301 gacccgccgg tgtctggaat tgcgctgtgc ggactccgtt gtcgtcgctg cccgccatca
      361 ctcgccagcc cggtgactcc ttgacgtagt tgcagtgttc gcattcgccg agcccgttct
      421 cggcggtggt ggggccgtcg cggtgatggg gccgggcgtg gtcgcagtgc cggatcggcg
      481 cgtcgcagta gggtgtgcgg caggtttgat cgcgcagccc gatgaagcgc gccaacccgc
      541 gcgggaagca ccgcgaccgc gactccatcg ccaccagctg ccccgacttg gggtggcggt
      601 agagccggcg cagcgtgcac cgcgcccgcg tgtcggtgac cgcccgctgg accagccggc
      661 gcgccaccgc agccggaatc ggcccgtacc cgctgaccgc ggccgcgctg ttgtcgccgc
      721 ccagcagcgt ctcgtcgccg atcaccaggt tgaccgccac cgcatcagcg acctcggccg
      781 gttgaccggt gacccgctcg aagaagacca acgcacctcc gtcgcgatgc tcgaacatac
      841 attcgaatat agtttggagt ctgacaagtc tcctcagccg tgccgggcgg cctactgggc
      901 cagcagcgcg acacgcgacg tcatgccgag ctttgagtag acgtgagtcg ggtgtgtctg
      961 cacggtgcga taggacacga acagccgcga ggcgatgtcc ttgttgccga gtccctcggc
     1021 gaccagtcga acgacgtcgt gctccgtcgg cgtcaaggac gcccaagcgg atgtgtgccg
     1081 aggtcgttca gccaggcgtc ctcaggcagg tgatcgacga ccatcgcctc ggtggcgccc
     1141 gacaacagcg tctgagcgcc gtgacccaga tcccgaagcc tcgccgtgcg gttgatcgtc
     1201 gacccgatgt cgttaccctc gtcgcgcaac tggatctcgc ccgtattcac gccgatgcgc
     1261 agccggatcg gcgcaagcgg cgcgctgacg gcggcgccgg cgatgcgtgg gcgggtgctc
     1321 gcactgctgg tcgttgccat cggaggcacg acaccgatcg gcgcgccgct gatgggatgg
     1381 ctcgccgagc tgttcggcgt gcgggccacc ttcatgctcg cgggcgtgct caccgcgatc
     1441 gcggcggcac ttgcctacgt ctatctgcgc cgcaccgccg atgagcccga cgactcatcg
     1501 gttcaccact cgcctttgag ccgggcgcgg taggaccggc cgtccgggtc atagaacgcc
     1561 cggtacgccg gttcggccca tgaccgagtc agcgcgccca gtttttccgg ccggtgcgga
     1621 cgcagccggc cgggcggccg ggggcccgtg cggccgttga catgccacat ctcgagcgcc
     1681 tgtgccgtcg cctcgatcgt cgtcactacc gtgtcgaggt cgacgagccc gtcgtcgtcg
     1741 ctgccgtcgg acgcgcggtc gaggtgctcg cgaatcagtc gcagccgcag atcccgtgcg
     1801 aactctccgg tgtcatccag tacggcacag gacagttcgc tgtcatgagt ccacgacctc
     1861 cggttcaagt tgtcgctgcc gaccgtggcc catacgtcgt cgatcacgca caccttcgcg
     1921 tgcacatata cgggtgtccc ccactggttt tcgacgtcga agatgtgcac ccgttcggga
     1981 tccgccgctc ggcaggcctc gatcgcctgc tgtcgtccaa cgaggttggg cggcagagac
     2041 agtcggccgt cgacatccgg atatcgcggc acgaccgcaa ccaggtgcag ttcgggattg
     2101 tcgttcagcg cgcgggcgaa caggtcggcg acccgcttcg accacagata ctggtcctcg
     2161 gtgtagatga gccgtctggc gcgcctgacg gccttggaat atccacgcgc gatgctgcgc
     2221 tcgccgtgcg gtgcgaagtc gtactcgaag tgtgcatccg ggtatgtgcg gagcacctgc
     2281 accgagtgtg gaccgcacgg cggcggatcg gcaggttgat ccggcagcgc gcccggcgtc
     2341 agatccgcgc cgcggagttt gtccgcgagc cacgcgatcg gcgagagcat gtcgagcgct
     2401 gccggatcgt tccatcgttc ccggaaggtc gtgtccaacg cgccgacgac cggaccccgc
     2461 agcgcaagct ggacgtcatg ccacggcggg tggtccccgt actccttgga catctgctgc
     2521 gcctgccggt caccgcggtg ggtttcgtcg tcgcgacggg agtggcacag gtcgatgccg
     2581 cctgcgaacg cgacatcgcg ctcgggttcg tcccgatgcc ggatgacgac cagcttctga
     2641 tggtgagaac cgccgaagcg gacgcgctgg tctagtaaca cctctccgcc cgcacgctcg
     2701 atcgcctcgc cgagatgctg gttctcctcc tcgctgtaag cgaatttgtc gagatgcgag
     2761 cgccacacca aacccttgac gacgacgcca cgctcggcgg ctgagcagaa cagttccctg
     2821 atggtcgggc cgtcgtcgcg catcttctcg tcggggtcgc cgcgccagtc ggtgaagaac
     2881 aggtagtcgc cctcaccgag catctcgacc tcgctgacca gccggtcgaa atacgtcgcg
     2941 ccgtgaatca gcggctcggc gcggctacct tcgcaccaag cgggaatcct gctcgccggg
     3001 ttgcctcgct cagccgctgt gaggaaccag tctgcgagct gtgacacgcg acccggatag
     3061 ccgtaggtga tcatgtccaa acgcaccgcc gccgcacgcg gtctgtcaag atcaccgcat
     3121 gcccgcagtc cgtcgcgccc tcgcactggc ctgcgcgctt ctggtgcttg ccggttgtgc
     3181 acccacaacg ccccccgaga aggaatcgcc tgcgccgacc accgatccgg ccaaggcgga
     3241 tgcggtgatg acgatcgtgc gcgacacgat ggcgcagaag catttgaaag cggtgctcgt
     3301 ccgggtgacc gtcgacggca aggaggtcgt cacacgggcc gtcggcgact cgatgaccaa
     3361 cgttcccgcg tcgaccgaca tgcatttccg caacggcgct gtggctatct cctatgtttc
     3421 gacgcttctg ctgatcctcg tcgacgagaa gaaggtcagc ctggacgaca agctgtcccg
     3481 atggctgccc gacgtaccg
//
LOCUS       DASYLX010000309         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_122817,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000309 DASYLX010000000
VERSION     DASYLX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122817"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1194
                     /locus_tag="VGP27_07515"
     CDS             19..1194
                     /locus_tag="VGP27_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851196.1"
                     /translation="MPAGDVADVFAGVEDRVPEALADHLRGIALIDHHVHGAFNKPVD
                     RATFEASINEGSTDRVPDFMTMFDSPLGLAIRRWCAPVLGLPRHAAPDDYWARRSELG
                     PDELTATMLAGAGVARWVVDTGFKGDMLTTLDQLTELSGRPASEILRLERLTEDLLEA
                     GTDPDDFPAAFRAAMQEAANSQHIVGTKTIAAYRTNFDIDWSRPTDMTVVEHARTLAS
                     QGNGLRVDDPVLIAFGVHEAAEHGLPIQVHVGFGDRDLDLNRTDPLLLLPLLRAMTPV
                     PVLLLHCYPFHRQAGYLAQAFDHVNFDVGLAINYLGSRSTALVGEALDTAPFAKQLYS
                     SDAFGPPELHLLGAVLWRRAMGLVLGEWVRTGECGEQDAIAIVDMIGVHNAARVYSL"
     gene            1412..>1826
                     /locus_tag="VGP27_07520"
     CDS             1412..>1826
                     /locus_tag="VGP27_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851197.1"
                     /translation="MRSGSGPVLNRVHGVQPELAGAGHVLCITTIGNVDADMDRLAVP
                     PETVLTPREAFFAADQAVPLRQASGRVAAKAITPYPPGIPLVMRGERLDDDVIALLSA
                     LRDAGNPIMHRIRRWKWCSRSAERDLSSGGWAPSGR"
BASE COUNT          278 a          612 c          610 g          326 t
ORIGIN      
        1 ccgaggaggt cgcccgttat gccggctggt gatgtcgcgg acgtcttcgc gggcgtcgag
       61 gatcgagtgc ccgaggcgct ggccgatcac ctgcgcggca ttgcgctcat cgaccaccac
      121 gtgcacggtg cgttcaacaa accggtcgac cgggcaacct tcgaggcgtc gatcaacgaa
      181 ggttcgacgg accgcgttcc cgacttcatg acgatgttcg actcgccgct cggtctcgcg
      241 atccggcgct ggtgcgcacc ggttctcggg ctgccacgac atgcagcccc cgacgactac
      301 tgggcgcggc gcagcgagct tggccctgac gagctgaccg ccacgatgct ggccggggcg
      361 ggtgtcgcgc gctgggtcgt cgacaccggg ttcaagggtg acatgctcac caccctcgat
      421 cagctcaccg agctcagcgg caggcctgcc tcggagatcc tgcgtctgga gcggctcacc
      481 gaggatctgc tcgaggccgg caccgatccc gacgactttc ccgcggcatt ccgggcagcg
      541 atgcaggagg cggcgaactc gcagcacatc gtcggtacca agaccatcgc cgcctaccgc
      601 accaacttcg acatcgactg gtcccggccg accgatatga cggtcgtcga acatgcacgc
      661 acacttgcct cgcagggcaa cggattacgc gtcgatgacc cggtgctgat cgccttcggt
      721 gtgcatgagg cggccgagca cggcttgccg atccaggtac acgtcgggtt cggcgaccgg
      781 gatctggacc tgaaccgcac cgatccgctg ttgttgctgc cgctgttgcg cgccatgaca
      841 cctgtgccgg tgctgctgct gcactgctac ccgttccacc gtcaggccgg ctatctggcg
      901 caggcgtttg atcacgtgaa tttcgatgtg ggccttgcca tcaactacct cggtagccga
      961 tcgaccgcac tggtcggcga ggcgctcgac accgccccct tcgccaagca gctgtactcg
     1021 tcggatgcct tcgggccgcc tgagctccat ctgctcggcg ccgtgctgtg gcggcgcgcc
     1081 atgggccttg tgctcggcga gtgggttcgc acgggcgagt gcggtgaaca ggatgcgatc
     1141 gcgatcgtcg acatgatcgg cgtgcacaac gccgcgcgcg tgtattcgct ctgatgaacg
     1201 acgccgcacc gcttttcgat gcactgaggc ggttcgtcga acgggattag gcgccgttct
     1261 acagtcccgg tcacaagggt ggccgcacgc tcgatccgtg gtttcgggag aacatcgcag
     1321 ggctcgaccg gcgggaacat cgccgtcgca ctggcgtcgc tacgggccga tcagccggtg
     1381 ctcgtagccc gcaacgcgca caagtcggtg cttgcggtct ggttcaggtc cggtgctcaa
     1441 ccgggtgcac ggtgtgcaac ccgaattggc gggcgccgga cacgtgctgt gcatcaccac
     1501 gatcggcaac gtcgacgccg acatggaccg gctcgcggtc cctccggaga cggtgctgac
     1561 gccgcgcgag gcgttcttcg ccgccgacca ggcggttccg ctccgacagg catcgggacg
     1621 cgttgcggcc aaggcgatta cgccctaccc gccgggtatt cccctggtga tgcggggcga
     1681 acggctcgat gacgacgtca tcgcgctgct gtcggcgctt cgcgatgcgg gcaatccgat
     1741 catgcatcgg atccgacgct ggaagtggtg ctcgcggtcc gctgagcgcg acctcagcag
     1801 cgggggttgg gccccttctg gccggc
//
LOCUS       DASYLX010000310         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3633031,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000310 DASYLX010000000
VERSION     DASYLX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633031"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGP27_07525"
     CDS             complement(<1..195)
                     /locus_tag="VGP27_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851198.1"
                     /translation="MAPPVVPSRPGQLDVFRRGDAYVRGCGAHTRSTKIAEIELIGVP
                     FDGYGRPGNQARAAAALRDAG"
     gene            219..1055
                     /locus_tag="VGP27_07530"
     CDS             219..1055
                     /locus_tag="VGP27_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEV7851199.1"
                     /translation="MSFTHGCVTEAVELGPRLRRIVVQVDDLAALDVKPAADSAVGVY
                     FPVASPDGTSHIDPDAEGRNYSVRHHAGDLITLDVFLHAYGVGTAWAAAARSGDRIGL
                     DHARFWYRPPRATDWLLLVTDLSGLPATARIVEESSSSHIPVTAIVEVADSGDLAYLP
                     PHTGMAVIPAVGTGNGHAPSRLAELVREHRLPAGRGYCWFAGEAAESRVVRKYFRGLG
                     WTIDQLDITGYWRFDSETWDARFAKVEDEVVAVYAQAIADCKGDKVASEEFDEALERV
                     GL"
     gene            complement(1118..1891)
                     /locus_tag="VGP27_07535"
     CDS             complement(1118..1891)
                     /locus_tag="VGP27_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV7851200.1"
                     /translation="MRSTPRCWLTDMDGVLVREEHALPGAAEFLHRLVEKERPFLVLT
                     NNSIFTPRDLSARLSRTGLSVPEAAIWTSALATSAFLADQLPGGSAYVIGEAGLTTAL
                     HEAGYTLTDVDPDFVVLGETRTYSFEAITKAVRLILGGARFIATNPDVTGPSAEGPLP
                     ATGSVAAMITKATGREPYFVGKPNPMMFRSALNRIEAHSENTCMVGDRMDTDVVAGIE
                     AGLETILVLTGSTTSEEVERYPFRPSRVLPSIAEAIELV"
BASE COUNT          305 a          672 c          632 g          318 t
ORIGIN      
        1 cccggcatcg cgcagcgcgg ctgccgcccg cgcctggttg cccggccgcc catagccgtc
       61 gaacggaact ccgatcagct cgatctcggc gattttcgtg ctcctcgtgt gcgcaccaca
      121 accccgaaca taagcgtcgc cccgccgaaa aacgtccagt tgaccgggtc tggacgggac
      181 tacgggaggg gccaagtcgg ttaggtaacc taagatcggt gagcttcacc catggttgcg
      241 tcaccgaagc ggttgagttg ggtccgcgcc ttcgccgaat cgtcgtgcag gtcgatgacc
      301 tcgccgccct cgacgtgaag ccggccgccg actcggccgt cggcgtctac tttccggttg
      361 cgtcacccga cgggacgagc cacatcgacc ccgatgccga gggccgcaac tactccgtac
      421 gacaccacgc aggcgacctc atcacgctcg acgtcttcct gcacgcgtac ggcgtcggaa
      481 cagcgtgggc ggcggcggcc cggtccggcg accggatcgg tctcgaccac gcccggttct
      541 ggtaccggcc gccacgggcc accgattggc tgctgttggt caccgacctg tcggggttgc
      601 cggccaccgc gcgcatcgtc gaggagtcgt cgtcctcgca catcccggtg accgcgatcg
      661 tcgaggtcgc cgacagcggc gacctggctt acctgccgcc gcataccggt atggccgtca
      721 tccccgccgt gggcaccggc aacggtcacg ccccgagccg actcgcggag ttggtccgcg
      781 agcatcggct gcccgccggc cgcggctatt gctggttcgc gggcgaggcc gccgaatcgc
      841 gggtcgtgcg caagtacttc cgcggcctcg gctggaccat cgaccagctc gatatcaccg
      901 gctactggcg cttcgactcc gaaacgtggg atgcgagatt cgccaaggtc gaagacgagg
      961 tcgttgcggt ctacgcgcag gccatcgccg actgcaaggg cgacaaggtg gcctccgagg
     1021 agttcgacga agctctggaa cgagtcggcc tctaggcacc gtgaccgcgt gagtcgcccg
     1081 gatcgccgcg aggacctcag tcgccgggcg acggcaatca caccagctca atggcttcgg
     1141 cgatgctcgg caacacgcgg ctgggccgga acgggtagcg ctcgacctct tcgctggtgg
     1201 tcgacccggt cagcaccagg atggtctcca gcccggcctc gatgccggcg acgacgtcgg
     1261 tgtccatccg gtcgcccacc atgcaggtgt tctccgaatg cgcctcgatt cggttcagcg
     1321 cactgcggaa catcatcggg ttcggcttgc cgacaaagta cggctcccgt ccggtcgcct
     1381 tggtgatcat ggccgcgacc gatccggtcg cgggcagcgg cccctccgcc gacggcccgg
     1441 tcacgtccgg attggtcgcg atgaaccgcg cgccgccaag gatcagccga accgccttgg
     1501 tgatcgcttc gaacgagtag gtgcgtgtct cgccgagcac gacgaaatcc ggatcgacat
     1561 cggtcagcgt gtatcccgct tcgtgcagcg cggtggtcag gcccgcttcg ccgatgacgt
     1621 aggccgatcc tcccggcaac tgatcggcga gaaatgccga cgtcgccaat gcggacgtcc
     1681 agatcgccgc ctccggaacc gagaggcccg ttcgcgacag cctcgccgac aggtcgcgcg
     1741 gggtgaagat cgagttgttg gtcaggacga gaaacgggcg ttccttctcg acgagccggt
     1801 gcaggaactc ggcggcgccc ggaagggcgt gctcctcgcg gaccagcacg ccgtccatgt
     1861 cggtcaacca gcaccggggc gtcgatcgca cgcgctcagt ctcccagttt tatccgcggg
     1921 ttctcgg
//
LOCUS       DASYLX010000311         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3852800,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000311 DASYLX010000000
VERSION     DASYLX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852800"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGP27_07540"
     CDS             <1..552
                     /locus_tag="VGP27_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851201.1"
                     /translation="ESQAQTRAQLVATARRMFFEDGYHPTSLEKVADAAGYSKGAVYS
                     NFRNKDELCAAVLDEIRAERFAEVIGLLSQPDRFEALRGWAERVIGDPGWTPLEAEFA
                     LHARSNDQLRSELAGRLGNIVDMLAAGVETADDLDVKMPSREAATVMLALGVGLGLMR
                     SIDPSIPVKGLIDTLRLVAGESS"
     gene            582..1004
                     /locus_tag="VGP27_07545"
     CDS             582..1004
                     /locus_tag="VGP27_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEV7851202.1"
                     /translation="MTEPQFGGNEGPPPPHQSQYQPPPPGYAPQQAGYPPPGQYGQYP
                     PAYMDPAAPYGRHPLTGEPLSDKSKIVAGLLQLIGLFGLVGIGRIYLGYTGLGIAQLV
                     VGLVTCGIGAVVWGIIDAVLIFTDKVRDPEGRPLREGT"
     gene            991..1407
                     /locus_tag="VGP27_07550"
     CDS             991..1407
                     /locus_tag="VGP27_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEV7851203.1"
                     /translation="MRAPSATVSSRARLYGALGSGGLLGGALAYIGLADPHNPDFVFP
                     ACPFKLLTGWNCPACGGLRMTHDMLHGDLAAAAVDNIFLLVGLPALLAWVLVRWRTGR
                     PIFTMPAIVVIAVLAVTWTVIRNLPGFPLVPTVLDG"
     gene            1494..>2284
                     /locus_tag="VGP27_07555"
     CDS             1494..>2284
                     /locus_tag="VGP27_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEV7851204.1"
                     /translation="MLFSALPHAQGLYDPERETDSCGVAMIADIRGRRSHAIVADGLV
                     ALEHLDHRGAAGAETNSGDGAGILIQLPVELFREVVEFELPPATAEGCNAFAAGICFL
                     PRDSDLRAAAREQIDAIAAEEGLQVLGWRDLPVDPDGADLGATALGCMPHMAQVFLAA
                     PDGVGGIDLDRRVYPVRKRAESGNVYFPSLSSRTIAYKGMLTTVQLPQYFSDLRDERC
                     MSAIAIVHSRFSTNTFPSWPLAHPFRFVAHNGEINTVRGNRNRMHA"
BASE COUNT          357 a          771 c          744 g          412 t
ORIGIN      
        1 gagagccagg cccagaccag agcgcaactt gtcgcgaccg cgaggcggat gttcttcgag
       61 gacggctatc acccgacgtc actggagaag gtcgccgacg ccgccggcta ctcgaaaggc
      121 gcggtgtact ccaacttccg caacaaagac gaactctgcg ccgcggtgct cgacgagata
      181 cgcgcggaac gattcgccga ggttatcggg ctgctttccc agccggacag gttcgaagcg
      241 ctgcgcggtt gggcggagcg cgtcatcggc gacccggggt ggacgccgtt ggaagcggag
      301 ttcgcgttgc acgcgcgctc caacgatcag ttgcgcagcg agctcgccgg gcgcctggga
      361 aacatcgtcg acatgctcgc cgccggcgtg gagaccgccg acgacctcga cgtgaaaatg
      421 cccagccggg aggcggccac cgtcatgctg gcgctcggtg ttggcctggg cctgatgcgg
      481 tcgatcgacc cgtccatacc tgtcaaaggc ctgatcgaca ctctgcggct ggtggctgga
      541 gaatcctcct agctaccctc cggttctggc atgctgatgc catgactgag ccgcagttcg
      601 gtggcaacga gggacctccg cctcctcatc aatcccagta tcagccgcca cctccgggtt
      661 atgcgccgca gcaagccgga tatccaccgc cgggtcagta cggccagtat ccgcccgcgt
      721 acatggatcc cgcggcgccc tacggccgcc acccccttac cggtgagccg ctttcggaca
      781 agtccaagat cgtcgcaggc ctgctgcagt tgatcgggct gttcggcctc gtcggcatcg
      841 ggcgcatcta cctgggctac accggcctgg gcatcgccca actggtcgtc ggcctcgtca
      901 cctgcgggat cggtgcggtg gtgtggggca tcatcgacgc cgtgctcatc ttcaccgaca
      961 aagtgcgtga cccggagggg cgcccccttc gtgagggcac ctagcgccac ggtcagcagc
     1021 cgggcgcggt tgtacggcgc gctcggttcg ggcgggctgc tcggcggcgc cctggcctac
     1081 atcggtctgg ccgatcctca caaccccgac ttcgtctttc cggcatgccc gttcaagctg
     1141 ctgacgggat ggaactgccc cgcctgcggc gggctccgga tgacccacga catgttgcac
     1201 ggcgatctgg ccgccgccgc ggtcgacaac atcttcctac ttgtcggact gcctgcgctg
     1261 ttggcttggg ttctggttcg gtggcgtacc ggacgcccga tcttcacgat gcccgcgatc
     1321 gtcgtcatcg ccgtgctcgc ggtcacgtgg accgtaatac ggaaccttcc ggggttcccg
     1381 ctggtcccga cggttctcga cgggtgaccg ccgaggcgcg ctgatacaat ttcagtagcg
     1441 ggccgctgca gtcccggacg acgaaattcg ccagactgcg gaggaaaatt cacgtgctgt
     1501 tctcggcatt gccgcacgcc caaggcttgt acgacccgga gcgcgaaacc gactcctgtg
     1561 gggtcgccat gatcgccgat attcggggta ggcgttcgca cgccatcgtc gccgacggtc
     1621 tcgtcgccct cgagcacctc gaccatcgcg gggcggcggg cgcggagacc aacagtggtg
     1681 acggcgcggg cattctcatt cagctgccgg tggaactctt tcgcgaggtc gtcgagttcg
     1741 aacttccgcc ggcgaccgct gagggctgca acgccttcgc ggcggggatc tgcttcctgc
     1801 cgcgggattc cgacttgcgg gcggcggcgc gcgaacagat cgacgccatc gcagcggagg
     1861 aaggtctgca ggttctcggc tggcgtgatc tgcccgtcga ccccgacggt gccgacctcg
     1921 gtgccacggc gcttggctgt atgccgcaca tggcgcaggt cttcctggcg gcgcccgatg
     1981 gggtcggcgg tatcgacctc gaccgtcggg tctacccggt gcgcaagaga gccgagtcgg
     2041 gcaacgtcta ctttccttcg ctgtccagcc ggaccattgc gtacaagggc atgctgacga
     2101 cggtgcaact gccgcagtac ttttcggatc tacgcgacga gcgctgtatg agcgcgatcg
     2161 ccatcgtgca cagccggttc tcgaccaaca cgttcccgtc ctggccgctg gcccacccgt
     2221 ttcgtttcgt cgcccacaac ggcgagatca acacggtgcg cggcaaccgc aaccgcatgc
     2281 acgc
//
LOCUS       DASYLX010000312         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1329504,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000312 DASYLX010000000
VERSION     DASYLX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329504"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGP27_07560"
     CDS             <1..318
                     /locus_tag="VGP27_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEV7851205.1"
                     /translation="LWAMFGLLPPDQEQRPHRHQSVALDLILDAKPGCYTLLGTRLDE
                     RGDIINPTRVDWEAGGAFVTPPGLWHAHFNESGAPAHLIPIQDAGLQTYLRSLDIRFS
                     DRR"
     gene            complement(319..>2464)
                     /locus_tag="VGP27_07565"
     CDS             complement(319..>2464)
                     /locus_tag="VGP27_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7851206.1"
                     /translation="LSMTQKLSRLPGRMQGGNFASVTSDVEVQPMRARVLSLVLRPIS
                     PPLALGVAVAAALLATESLVVYLLMQVVPGNLFGVVFLLGVLVVSLGWGFRLGTTTAL
                     ASALVYGYFHYLQTGGSFVPTDPRHWVAVAVFLVIALSATALAGVARLRAVAADERRR
                     EVEASHDDLSVLADHQAALRRVATLVARGIVPSELFCAVAEELARCLGTYHHAVLFRY
                     ESESTGVLLAAGHGDSGLKAIPVGQRFSLEGESVAAQVARTCRAARMDSYEHASGSVA
                     ASFRDLGLRSAVGAPIIVDGDLWGAAIVGSTKPAPLPSDTEARVGDFTDLVATAIANA
                     QTRADLAVLAEQQTALRRVATLVARGEHPSELFSAVAGELARCLGVHHSTLFRYESDG
                     TATLLAARHEPGLKTIPVGRRFSFAGDNIAAMVYEAGRTARMDSHDNAAGLAAEYIRE
                     IGIRSSVGAPIIVDGRLWGVAIVGSSRPEPLPSDTEARVGDFADLVATAIANAHTHAE
                     LTASRVRIVAAADDARRRIERDLHDGAQQRLVSLGLELRTAEAGVPAQLHPLKEQIDH
                     LVTKVAEISTELQELSRGIHPAILSRGGLSPALKVLARRSAVPVELVLAVDKRLPDSA
                     EVAAYYVVAEALTNAAKYARATEVQVCLDTEGPNLHLSVRDDGIGGADTAKGSGLVGL
                     IDRVEALGGTLSISSHPGNGTSLDVNIPFEIE"
BASE COUNT          440 a          840 c          799 g          385 t
ORIGIN      
        1 ttgtgggcaa tgttcggtct gctgccgccg gatcaggaac aacgtccaca ccgccaccag
       61 tcggtcgcgc tcgatctgat tctcgacgcc aagcccggct gctacacgct gctgggcacc
      121 cgactggacg agcggggcga catcatcaac ccgacccgtg tcgattggga ggcgggcggc
      181 gcgtttgtca cgccacctgg cctgtggcac gcgcacttca acgagtcggg cgcgccggcg
      241 catttgatcc cgatccagga cgcgggcctg caaacctatc tgcggagcct ggacatccgg
      301 ttctccgacc gccggtgatc actcgatttc gaaagggatg ttgacgtcca gcgaggtgcc
      361 gtttccgggg tggctcgaga tcgacaaggt gcccccgagc gcctcgacac ggtcgatcag
      421 accgacgagt ccagatcctt tggctgtgtc ggcaccgccg attccatcgt ctcgaaccga
      481 gagatgcaga ttggggccct cggtatcgag gcacacctgc acttcagtcg cccgagcgta
      541 tttggctgca ttcgtaaggg cctcggccac aacgtaatac gccgccacct cggcagagtc
      601 cggcaaccgt ttatcgacag cgaggaccag ctcgacgggg accgcggatc ggcgggccag
      661 gaccttgagc gctgggctca gtcctcccct ggacaggatc gccggatgaa tcccgcggga
      721 gagttcctgt agctcggtcg agatttcggc cacttttgtc acaaggtggt caatctgctc
      781 cttcagcggg tgcagttggg caggcacgcc cgcctccgcg gtacgcaact cgagcccgag
      841 cgagaccaac cgctgttggg cgccgtcgtg cagatcgcgt tcgatacggc gccgggcgtc
      901 gtcggcggcc gcgacgatcc ggacccgcga cgcggtgagc tcggcgtggg tgtgggcatt
      961 tgcgatcgca gtcgccacca gatccgcgaa gtctccgacg cgcgcctcgg tgtctgatgg
     1021 cagcggctcg ggtcgagacg agcccacgat cgccacaccc catagccttc cgtcgacgat
     1081 gattggcgct ccaaccgacg agcggatgcc tatctcacgg atgtactcgg cggccaatcc
     1141 agcagcgttg tcgtggctgt ccattcgggc ggtgcggccg gcttcgtaga ccatcgccgc
     1201 gatgttgtcg cccgcgaatg aaaaccgcct accgacggga atcgtcttca accccggctc
     1261 gtggcgggcc gcgagcaatg tggccgtgcc atcggactcg tagcggaaca gcgtcgagtg
     1321 gtgcacgccc agacaccggg ccaactcccc cgccactgcg gagaagagct cagacgggtg
     1381 ttccccgcgc gcaaccaacg ttgccaccct tcgcagggca gtctgttgct cggcgagcac
     1441 agcgaggtcg gcgcgggtct gggcgttggc gatcgcggtc gcgaccagat ccgtgaagtc
     1501 cccgacgcgc gcctcggtat cggaaggcag cggagccggc ttcgtcgagc cgacgatggc
     1561 cgcgccccac aggtcgccat cgacgatgat cggcgcgccc acggctgagc ggagtcccag
     1621 atcgcggaag ctcgccgcga cggacccgga agcgtgctcg tagctgtcca tccgggcggc
     1681 gcgacaggtg cgggccacct gtgccgcgac gctttcgccc tcgagtgaga accgctgccc
     1741 caccggaatc gccttgagcc cactgtcacc atggcccgcg gccagcagaa cgcccgtgct
     1801 ctcggactcg taccggaata acacggcatg gtggtacgtg cccaggcacc gcgccagctc
     1861 ctccgccacc gcgcagaaca gctccgacgg gacgatcccg cgagcaacca gtgtcgctac
     1921 ccgtcgcaac gcggcctggt gatcggcgag cacactgaga tcgtcatggc ttgcctcgac
     1981 ctcccggcgg cgctcgtccg ccgcgacggc gcgcaatcgg gccacgccgg cgagagcggt
     2041 ggcggacagc gcgataacca ggaagacagc gacggccacc cagtgccggg ggtcggttgg
     2101 gacgaagctt ccgcctgtct ggagatagtg gaagtacccg tagacgaggg cactcgccag
     2161 cgccgtcgtt gtcccgagcc tgaatcccca ccccagcgaa accaccagca cgcccagtag
     2221 gaagaccaca ccgaaaaggt tgccggggac gacctgcatg agcagatata cgacgaggct
     2281 ctctgtcgcg aggagtgccg ccgcgaccgc gacccccaga gcaagaggtg ggctgatcgg
     2341 acgcaacacc aaagacagca cgcgtgcgcg catcggctgc acttctacat cggacgtgac
     2401 gctcgcaaaa ttgccaccct gcatccggcc gggcagccgc gacagcttct gcgtcataga
     2461 caag
//
LOCUS       DASYLX010000313         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1439476,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000313 DASYLX010000000
VERSION     DASYLX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439476"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VGP27_07570"
     CDS             complement(<1..844)
                     /locus_tag="VGP27_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEV7851207.1"
                     /translation="MTDIAVVGLDCRFPKGGDPAALWKLLLEGADGIDDIPVERWDAA
                     ELLGDGAVNHRAGGLICDADAFDNDFFGITPREAEAMDPQQRLLLQTAWRALENATLD
                     PRGQAGSNTGVFVGVMANEWAHLHMSDYRAITAQDGSGNGYFMTANRLSYQLDFNGPS
                     LAVDTACSSSLVAVHLAVQALRNAECEQAVAAGVNLTLTPALNVFYTKAGLAAPDGRC
                     KPFSGKADGIGRGEGVAVVVLRRLEDAVAAGLPIYAVIKGSAVNSDGRSNGITAPNRW
                     AQQQV"
     gene            complement(841..2592)
                     /locus_tag="VGP27_07575"
     CDS             complement(841..2592)
                     /locus_tag="VGP27_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005241881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7851208.1"
                     /translation="MTTGDVTVGWAPRIGQKSLVAQLRSQFEVHAGNRHLTFLNESGR
                     HLVPEVTSFGDLDRDAREMAAWLSSRPEAKRPVLLLFEPGIDFWRAFVGCLYAGVVAV
                     PTPLPIDERSMKRVAGILRDADSSMALTTERLHGLLTAGIEGLDLGRPLRCVASNDAA
                     LADPDSWAMPQLTPETVAFLQYTSGSTGDPKGVAVTHGNLLDNEATMSQALRVSATSV
                     LVGWIPHFHDMGLIGQLLAFFNGASLITMSPLAFLKQPIRLLKAMSDYRGTITAAPNF
                     AYDLIARRVTKEQLTGLDLSCWDVALNGAEPVRRRTIERITGVLAPAGFAPSAMRPAY
                     GMAEVTLMATFSSGVQRYLDADADALEQNRYEPATTGRATTLVSSGAPAPGVEVRIVD
                     PETLRELSDGDVGEIWLRSASVASGYHNRPAQTVEQFRAETSDGEGPYLRTGDLGLLH
                     DNELYVTGRLKDLLIANGRNLYPQDIEESVQDVHPAVAGSRGCAVSVDVDDAERLVLI
                     QAIKTEMLGDMNCSELAAAVKTAIARGFEIPAPTVVFVNRAGIHLTTSGKVQRASMRE
                     AFLKGELTDVVHEEHPR"
BASE COUNT          530 a          974 c         1055 g          573 t
ORIGIN      
        1 agacctgctg ctgcgcccag cggttcgggg cggtgatccc attgctgcgc ccgtcgctgt
       61 tgaccgcgct gcccttgatc acggcgtaga tcggcaagcc tgcggcgacc gcgtcctcga
      121 gccggcgcag cacgacgacg gcaacaccct cgccccggcc gattccatcc gccttgccgc
      181 tgaacggttt acagcggccg tccggagccg ccagccccgc tttcgtatag aagacgttca
      241 gcgcgggggt cagggtgagg ttgactccgg cggcgactgc ctgctcgcat tccgcattgc
      301 gcagcgcctg tacggccaga tgaaccgcaa caagcgacga cgagcacgcg gtgtccaccg
      361 cgaggctcgg tccgttgaag tccagttggt aggagagtcg gttcgcagtc atgaagtagc
      421 cgttgcccga accgtcctga gcggtgatcg cgcggtagtc gctcatgtgc agatgagccc
      481 attcgttggc catcaccccg acgaacaccc cggtgttgga accggcctgc ccgcgggggt
      541 cgagcgtggc gttttcaagc gctcgccacg ccgtctgcag gagcaggcgt tgctgcgggt
      601 ccatcgcctc cgcctcgcgc ggggtgatgc cgaagaagtc gttgtcgaag gcgtcggcgt
      661 cgcatatcag cccgcccgcc cggtggttga ctgcgccgtc gccaagcagt tcggccgcgt
      721 cccaccgctc gaccgggatg tcgtcgatgc cgtcggcgcc ctcgagcagc agcttccata
      781 gggcggcggg atcgcctccc ttggggaagc gacagtcgag gccgacgact gcgatgtcgg
      841 tcatcggggg tgctcctcat ggaccacgtc ggtgagttcg cctttcagga acgcttcccg
      901 catcgatgcg cgttgtacct ttccgctggt ggtgaggtga attccggcac ggttgacgaa
      961 cacgacggtc ggggcgggaa tctcgaagcc gcgcgcgatg gcggtcttga cggcggcagc
     1021 gagttcgctg cagttcatgt caccgagcat ctcggtcttg atggcctgga tcagcactag
     1081 gcgttctgcg tcgtcgacgt cgaccgatac agcgcagccg cgtgaaccgg ccaccgccgg
     1141 gtgcacgtcc tggactgatt cttcgatgtc ctgcgggtac aggttgcggc cgttggcgat
     1201 cagcaggtct ttcaggcggc cggtgacata cagctcgttg tcgtggagta gcccgaggtc
     1261 gccggtgcgc agataggggc cctcgccatc ggaagtttcc gcgcgaaact gttcgactgt
     1321 ctgtgcggga cggttgtggt agccgctggc gacgctcgcg cttcgcagcc atatctcacc
     1381 gacgtcgccg tcggataact cgcgcagggt ttcggggtcg acgatgcgca cctcgacacc
     1441 gggcgccggt gcgcccgagc tcaccagggt ggtcgcgcgg cccgtggtgg cgggttcata
     1501 gcggttctgt tcaagagcgt cagcgtcggc gtcgaggtaa cgctgcacgc cgctgctgaa
     1561 ggtggccatc aatgtcacct ccgccatgcc gtaggcgggt cgcatcgccg atggcgcgaa
     1621 cccggccgga gcaagcacgc cggtaatccg ctcgatggtg cgacggcgaa ccggttcggc
     1681 accgttgagc gcgacgtccc agcacgaaag gtcgaggccc gtcaattgct ctttcgtcac
     1741 ccggcgtgca atcaggtcgt aggcgaaatt cggtgcggcg gtgatggttc cgcggtaatc
     1801 gctcatcgct ttgaggagcc gtatcggctg cttgagaaac gccagtggcg acattgtgat
     1861 gaggcttgct ccgttgaaga acgccagcag ttggccgatg aggcccatgt cgtggaagtg
     1921 cgggatccag ccgacgagca ccgacgtcgc gctgacgcgc agggcttgcg acattgtggc
     1981 ctcgttgtcc aacaagttgc cgtgggtgac ggcgacgccc ttgggatcac cagtcgagcc
     2041 cgacgtgtat tgaaggaagg cgacggtctc aggggtgagc tgcggcatcg cccacgagtc
     2101 cggatcagca agcgccgcgt cgttgctggc aacacaccgc agcgggcgac cgagatccag
     2161 tccttcgatg cccgcggtga gcaggccgtg cagcctctcg gtggtcaacg ccatgctgct
     2221 gtccgcgtcg cgaaggatcc cggcgacacg cttcatgctc cgctcatcga tgggcagcgg
     2281 ggtgggcacc gcgactactc cggcgtaaag gcagccgacg aacgcgcgcc agaaatcgat
     2341 acccggctcg aataagagca gcaccggtcg cttggcctcg ggccgtgaac tcaaccaagc
     2401 ggccatctcg cgcgcatcgc gatcgagatc gccgaaactc gtcacttcgg gtacgagatg
     2461 gcgcccggac tcgttcagga acgtcaaatg gcggttgccg gcgtgaactt cgaactgcga
     2521 acgcagttgc gcaacaaggc ttttctgccc gatgcgcggt gcccacccaa cggtgacgtc
     2581 acctgtggtc atcgctgctc ctccgtatcg tcgccaagct ggtggctgga gcgtcgttca
     2641 gagcttgcgg ccgaggcggc gagccgaaga cgagacttgg cttagctggc gtgacctcac
     2701 cccgatgcac gccccctgca tatggcttga ggccggcgat gaggtgagtg atgcccccat
     2761 agtgaatcct gtccggcggc tatggcccgc cgagagtggg tcgcaaaagg atcatggctg
     2821 actccttgtg aaattggacg gcccaccaac agctggcgat ctcagcaaac cggcgcactg
     2881 atgatactgg cccctgccgc atcgtttgac aaccccgaat tactggcgat acgtgagcaa
     2941 tggcgaggca taggaatcga cggcttggca aattttgtgc cgtggtaagc aaccggagcg
     3001 ccgacaaccg ccgcggcggt ggtagtgcga cgggcgctgc ggtgtcgcct atgccacctg
     3061 ttgctgctcg gcgacggcgg tgccgacgaa atctgcaatg cgggagagag tggggtagtc
     3121 aaagatcatc gt
//
LOCUS       DASYLX010000314         5786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2536054,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000314 DASYLX010000000
VERSION     DASYLX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536054"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGP27_07580"
     CDS             <1..220
                     /locus_tag="VGP27_07580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV7851209.1"
                     /translation="GEEFDSSWNRGESIEFPLRGLIQGWQDGIPGMRVGGRRRLIIPP
                     EQAYGPAGSGHRLSGKTLIFLIDLLDVR"
     gene            complement(472..1812)
                     /locus_tag="VGP27_07585"
     CDS             complement(472..1812)
                     /locus_tag="VGP27_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7851210.1"
                     /translation="MTPLSRFLTRTPSLRTRVAFATAIAAAIVVGIVGTVVWIGITND
                     RKERLDRRLDEAAGFAIPFLPRGLDEIPKSPNDHDAVITVRKGEQVTSNSKVVLPRLP
                     AGYADTDVNGVRYRVRTVEIRLPEPMSVAVGATYDATIADTNNLHRRVLIICSLAIGA
                     ATFAGWLLAAFAVQPLKRLAQQTRQIDAGDAAPDVEVRGASEAVEIAEAVKGMLQRIW
                     NEQDRTKAALASARDFSAVSAHELRTPLTAMRTNLEVLSTLDLPDEQRKEVINDVIRT
                     QSRVEETLGALERLAQGELSTSDDHVPVDIAELLDRAAHDAMRVYPDLDVSLVPAPTV
                     IILGLPAGLRLAVDNAIANAVKHGGATRVQLSAVSSRAGVEIAIDDDGVGIPEEERDV
                     VFERFTRGSTASVSGSGLGLALVAQQAELHGGTASLQSSPLGGARLLLRLPGPQ"
     gene            complement(1870..2580)
                     /locus_tag="VGP27_07590"
     CDS             complement(1870..2580)
                     /locus_tag="VGP27_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7851211.1"
                     /translation="MSGMDSGAVSPRVLVVDDDDDVLASLERGLRLSGFDVSTAKDGA
                     EALRSATETRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSARSSVDDRVAGLE
                     AGADDYLVKPFVLAELVARVKALLRRRGATATFSSETISVGPLEVDIPGRRARVNGVD
                     VDLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEANG
                     APRLLHTVRGVGFVLRTQ"
     gene            2743..3720
                     /locus_tag="VGP27_07595"
     CDS             2743..3720
                     /locus_tag="VGP27_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7851212.1"
                     /translation="MPKLIVEPDDGLEPVNDFIESAKSSLLIKQFTFTEESFITAVIA
                     RKKAGVDVRVMLNPARSGGDRANDETYEQFKKAGVNVQWSNPSFYVTHEKSIVVDGKS
                     ALVATFNLCPKYFSHTRDYGIITTQPHRVAQIAEVFEADWAHREWTCSRYEGLLWSNS
                     NSRYHMAQFIDTAKQTLDIQHPKYVDAVILDRVGAAAERGVKVQVLCGGKHGISEWDI
                     LDTFASLRTLRRFGVKVHKQKNLRVHAKLLIADGEHALLGSMNIDRSAFDLRRELGIT
                     ITDHDVVGRLKGVFDTDWELSHHYEPPDPLDPSQHLEDDFPHDEDLIHE"
     gene            3717..4286
                     /locus_tag="VGP27_07600"
     CDS             3717..4286
                     /locus_tag="VGP27_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV7851213.1"
                     /translation="MSESPVDAERGEVSLFELAWTFNHIALASFGGGLSAWSREVLVV
                     EKEWMGEEEFLSAMTMCRILPGANQVNMAVFVGTKMKGVPGALASLLGLTLMPLVFVL
                     LMGFIYFRFKEVPAVKSVLHGASAAAVALTIAMVIQTGRKCLTSVVPVLLALACFVLN
                     GLVRWPLPVTLAMVAPLALLWAWPRRRAA"
     gene            4283..4828
                     /locus_tag="VGP27_07605"
     CDS             4283..4828
                     /locus_tag="VGP27_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV7851214.1"
                     /translation="MSTYLQLAGLFASLSLLSIGGGNAVLPEMHMQAVRGHNWLTDSQ
                     FADVFSISQAAPGPSILIVALVGYGAGLEVGGVPGAIVGGVVATVAMIVPAATLVYVL
                     TLFWQKAQKSRLRAAVEKGFAPLTVGLILATALVMGRAADHDWRAYLLTGVCTAIFIF
                     TRINPLIVVVVAGVIGYLGVV"
     gene            5050..>5786
                     /locus_tag="VGP27_07610"
     CDS             5050..>5786
                     /locus_tag="VGP27_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV7851215.1"
                     /translation="MSFETVARQTLYRQTHAKGGDLRSLADRRLLGRIWRFAARHHRR
                     LAVFVAVSVVSALLGLATPLLAGKVVDEIGNGAIGAVVWLAAVIAAVAVAEAGVSLVT
                     RWLSSIIGEGLILDLRTAVFDHVQRMPVAFFTRTRTGALVSRLGNDVMGAQRAFSDTF
                     SGVITNVVTLTLTMVVMLRISWQVTLLSLMLMPLFLLPARWIGRTMARLSREAAIHNA
                     TMNTQMTERFSAPGATLVKLFGSPATES"
BASE COUNT          932 a         1886 c         1941 g         1027 t
ORIGIN      
        1 cggcgaggag ttcgacagct cctggaatcg cggcgagtcc atcgagttcc cgctgcgggg
       61 gctcatccag ggatggcagg acggtatccc cggcatgcgg gtcggcggcc gccgccggct
      121 catcatccca cccgagcagg cgtacggacc ggcgggctcg gggcaccgat tgtcgggcaa
      181 gactttgatt ttcctgatcg acctcttgga tgtccgctaa ctgatggggc ggcgaatttt
      241 gcccggaccg taaggtcgcg ctggttcgcc acaccgacaa cagcggtaat gacgcggtca
      301 atatcccact gagcagcaac cgggccaaag ccgttgcgga ttaccttatt ccgcgaggta
      361 tcggcgcgga ccgcgtcacc tcgaagggtg tcagctaggc aagaactccg ccgagccggc
      421 ccgcttcaac cgctgggcca ccggtaaatc gcggcgcgag gaggagtagt gctactgcgg
      481 cccgggcagc cgcagcagca gccgcgcgcc gccgagcgga ctggactgta aagacgctgt
      541 gccgccgtgt aattcggctt gctgtgccac cagcgccagg ccgaggcccg agcccgagac
      601 cgaagccgtc gagcctcttg tgaatcgttc gaacacgacg tcgcgctctt cttcggggat
      661 gccgacaccg tcgtcgtcga tcgcgatctc cacacccgcg cgggaactca ccgccgacag
      721 ctgcacccgg gtggcgccgc cgtgcttgac cgcgttggcg atcgcgttgt ccaccgccag
      781 ccgcagaccg gcgggcaacc cgaggatgat gacggtcggc gcggggacca gcgacacgtc
      841 gagatccggg tagacgcgca tcgcatcgtg tgccgccctg tcgagcagtt cggcgatgtc
      901 gaccggcacg tgatcatctg aggtcgacag ttcgccctgc gccaggcgct ccaacgcgcc
      961 gagtgtttcc tcgacacggg actgggtccg gatgacgtcg ttgatgacct ctttgcgttg
     1021 ctcgtcgggc aggtcaaggg tggacagcac ctcgaggttg gtgcgcatcg cggtcagcgg
     1081 agtgcggagt tcgtgggccg agaccgccga gaagtcgcgc gccgaggcca gcgccgcctt
     1141 ggtgcggtcc tgctcgttcc agatgcgctg cagcatgccc ttgaccgcct cggcgatctc
     1201 gacggcttcg gaggcgccgc gcacttcgac gtctggcgcg gcatcccccg catcgatctg
     1261 gcgggtctgc tgcgcgagcc gcttcagcgg ctgaaccgcg aaggcggcga gcaaccaccc
     1321 tgcgaacgtc gccgcgccga tcgccaggga gcagatgatc aagacacggc ggtgaaggtt
     1381 gttggtgtcg gcgatggtcg cgtcgtaggt ggcgccgacg gccaccgaca tcggctccgg
     1441 caggcggatc tcgacggtgc gcacccggta gcgcaccccg ttgacgtccg tgtcggcata
     1501 gcctgcgggc agccggggga gcaccacctt ggagttcgag gtcacctgct cgcccttgcg
     1561 gacggtgatg acggcgtcgt ggtcgttggg cgacttcggg atctcgtcga gcccgcgcgg
     1621 taggaacggg atcgcgaacc cggcggcctc gtcgagccgc ctgtcgaggc gttccttgcg
     1681 gtcgttggtg atgccgatcc acacgaccgt gccgacgatg ccgaccacga tcgccgcggc
     1741 gatggctgtc gcgaacgcca cgcgggtgcg cagcgaaggg gttcgggtga ggaaccgcga
     1801 cagcggagtc atcggactac ggctttcggt tcttcgcgcg agcgtcgcgc aagccggcgg
     1861 tgccccacat cactgcgtcc ttagcacgaa ccccacgccg cgaacggtgt gcagtagccg
     1921 cggcgcgccg ttggcctcca gcttgcggcg gaggtagccg atgaacacgt cgaccacgtt
     1981 ggtgtcggcg gcgaagtcgt agccccagac cagctccagc aactgagccc gcgacagcac
     2041 ggcggtcttg tgctcggcca gcacggccag taggtcgaat tcgcgcttgg tcagatcgac
     2101 gtcgacgccg ttgacgcgcg cccggcggcc cgggatgtct acctcgagcg ggccgacgga
     2161 gatcgtctcc gacgagaacg tcgccgtcgc gcccctgcgt cgcagcagcg ccttcacccg
     2221 cgccaccagc tcggccagca cgaacggctt gaccagatag tcgtcggcac ccgcctcgag
     2281 accggcgacc cggtcgtcga ccgagctgcg ggccgacagc acacagaccg ggacgtcgtt
     2341 gtccatcgcg cgtagcgccg tgacgacgct gacgccgtcg agcaccggca tgttgatgtc
     2401 gaggacgatc gcatcaggcc tggtctcggt ggcgctgcgc agcgcctcgg cgccgtcctt
     2461 cgccgtcgag acgtcgaatc cggacagccg cagaccacgc tccagggacg cgagcacgtc
     2521 gtcgtcgtcg tcgacaacga gcactcgggg cgaaacagca ccactgtcca tgcccgacat
     2581 cttgcctgat cgaagcctgc gaatgggata ggcgcgagac gtacctgcta tcacgtgtgc
     2641 ggcggtcgcg tcaccgccat gtcacggtgc ccgcaacgtt catctgatag caaagcggcc
     2701 tggtctgcgc ggtgctagca ttcgacactt gtgccgacgt cgatgccaaa gctgattgtc
     2761 gaacctgacg acggcctgga accggttaac gacttcatcg aatccgcgaa gtcatcgctg
     2821 ctcatcaagc agttcacttt caccgaggaa agcttcatca ccgccgtcat cgcccggaag
     2881 aaggccggcg tcgatgtgcg ggtcatgctg aacccggcac ggtcgggcgg cgaccgcgca
     2941 aacgacgaga catacgagca gttcaagaag gcgggggtca acgtccagtg gtcgaatccc
     3001 agcttctacg tgacccacga aaagtcgatc gtcgttgacg gaaaatcggc gctcgtggcc
     3061 accttcaacc tctgcccgaa gtatttctcc cacacccgcg actacgggat catcacgaca
     3121 cagccgcatc gggtcgcgca gatcgccgaa gtgttcgagg ccgactgggc gcaccgcgaa
     3181 tggacctgtt cgaggtatga aggcctgctc tggagcaact cgaattcgcg gtatcacatg
     3241 gctcagttca tagacacggc caagcagacg ctggatatcc aacaccccaa gtatgtcgac
     3301 gcggtcattc tcgatcgtgt cggggccgcc gccgagcgcg gcgtgaaggt acaggtgctg
     3361 tgcggcggta aacacggcat cagcgaatgg gacatcctcg acaccttcgc gtcgcttcgg
     3421 accttgcggc gcttcggcgt caaggtgcac aagcagaaaa atctgcgggt gcacgcgaag
     3481 ctgctgattg ccgatggaga gcatgcgctg ctcggctcca tgaacatcga ccgcagcgcc
     3541 ttcgacctac ggcgcgagct gggcatcacg atcaccgacc atgacgtcgt cggccggttg
     3601 aagggcgtct tcgacaccga ctgggagctg tcccatcact acgaaccgcc tgatccgctc
     3661 gacccgtcgc agcacctcga ggacgacttc ccgcacgacg aagacctcat ccacgagtga
     3721 gcgaaagccc agtcgacgcc gagcgggggg aagtctccct gttcgaattg gcctggacgt
     3781 tcaaccacat agcgctcgcg tcgttcggcg gcgggctgtc ggcgtggtcg cgcgaggtgc
     3841 tggtcgtcga gaaggagtgg atgggcgagg aagagttcct cagcgccatg acgatgtgcc
     3901 ggatactgcc cggcgccaac caggtgaaca tggcggtgtt cgtcggcacc aagatgaagg
     3961 gcgttcccgg cgcgctggct tcgctgctgg gtctgacgct gatgccgttg gtgttcgtgc
     4021 tgctgatggg cttcatctac ttccgcttca aggaggtgcc cgcggtcaag agcgtgctgc
     4081 acggcgcgtc ggctgcggcc gtggcgctga ccatcgcgat ggtgatccag accgggcgca
     4141 agtgcctcac ctcggtggtg ccggtgttgc tggcgctggc gtgcttcgtg ctcaacggcc
     4201 ttgtgcggtg gccgcttccg gtcacccttg cgatggtggc tccgctcgcc ctgctgtggg
     4261 cgtggccgag aaggcgcgcg gcgtgagcac gtatctgcaa ctcgcggggt tgttcgcgtc
     4321 gctttcgctg ctgtcgatcg gcggagggaa cgcggtgctg cccgagatgc acatgcaggc
     4381 cgtccgtgga cataactggc tgaccgattc ccagttcgcc gacgtgttct cgatttccca
     4441 ggccgcgccc gggccgagca tccttatcgt cgcgctcgtc ggctacggcg ccggcctcga
     4501 agtcggcggt gttcccggcg cgatcgtcgg gggcgtcgtc gcgacggttg ccatgatcgt
     4561 gcccgccgcg accctggtgt acgtcctcac cctgttctgg cagaaggcgc agaagtcacg
     4621 gctccgcgcc gcggtcgaga agggcttcgc gccgctgacg gtcggcctca tcctggcgac
     4681 cgcgctcgtg atgggccggg cggccgatca cgactggcgg gcctaccttc tgacgggcgt
     4741 ctgcacggcg atcttcatct tcaccagaat caatccgttg atcgttgtgg tcgttgcggg
     4801 cgtgatcggc tacctcggag tggtgtgacg ggccggtgac agccggcgcg gtcgagcgtc
     4861 acccgggtgt cgcctcgacg caggaacccc agcgatcgac gttttggcgg ccttcactcg
     4921 cacttcctct gcggaatatc ggttgctgtg gcgcctgatt gcgccgaggg gacagccggc
     4981 gcggtttagc ctttgacgcg ctcgggaagt cgttgacctc atccattgtt gaggttttac
     5041 ggtgaattca tgagttttga aacggtcgca cgacagacgt tgtaccggca gacgcacgcc
     5101 aagggcggcg atctgcggtc gttggctgac cgcaggttgc tcggccgtat ctggcgtttc
     5161 gccgcgcggc atcatcgcag gctggccgtc tttgtggcgg tcagcgtcgt cagcgcactg
     5221 cttggtctgg ccaccccgct gttggccggc aaggtggtcg acgagatcgg caacggcgcg
     5281 atcggcgcgg tggtgtggct ggccgccgtg atcgccgcgg tcgccgtcgc cgaggccggc
     5341 gtatcgctgg ttacccgctg gctgtcgtcg atcatcggcg aggggctgat cctcgatctg
     5401 cgcacggccg tgttcgacca cgtgcagcgg atgccggtcg ccttcttcac ccgtacccgc
     5461 accggcgccc tggtgagcag gctcggcaac gacgtgatgg gcgcgcagcg cgcgttctcc
     5521 gacacgttct ccggcgtcat caccaacgtg gtcaccctca cgctgacgat ggtcgtcatg
     5581 ctcaggatct cctggcaggt cacgctgctg tcgctgatgc tgatgccgct gttcctgctg
     5641 cccgcccggt ggatcggccg aaccatggcg cggttgtccc gggaagccgc catccacaac
     5701 gcgacgatga acacccagat gaccgaacgc ttctccgcgc ccggcgccac gctggtcaag
     5761 ctcttcggca gcccggccac cgagtc
//
LOCUS       DASYLX010000315         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2098481,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000315 DASYLX010000000
VERSION     DASYLX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098481"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGP27_07615"
     CDS             <1..444
                     /locus_tag="VGP27_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HEV7851216.1"
                     /translation="TFAAIKAGRPTPEMLAYQYLQTLPQMAKGEANKVWLVPSDFGSA
                     LEGFTRMLGAPGEDGVFRYTPSPVDENLPKPEDDSAEVAEWFNTQTDPAIAQAVAKAE
                     ADARSSSGISAIPAAPEDPPPPMQYPPLSQQRQDGPPQSPGRHSG"
     gene            complement(332..1156)
                     /locus_tag="VGP27_07620"
     CDS             complement(332..1156)
                     /locus_tag="VGP27_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV7851217.1"
                     /translation="MKPVVSTLAAVRSAVISTAKQVPPRQLAAIAATIVILVAILLLV
                     PRPGAMQLRDWAQSLGPWFPLAFLAAHIVVTVFPFPRTAFTLAAGLLFGPWLGIAIAV
                     VASAVSALLALLLVRAAGWRLDRLVPHPRVKTLDARLRRRGWPTIMSMRLIPAVPFSV
                     LNYAAGASSVRVLPYTWATLVGLLPGTAAVVILGDALTDHISPLLILVSLFTASLGVA
                     GLIYEIRSYPQHHRVDDSPANRCDAPATAADRPAAAVTAADTASAAGGPPVRPEWR"
     gene            1256..>2361
                     /locus_tag="VGP27_07625"
     CDS             1256..>2361
                     /locus_tag="VGP27_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7851218.1"
                     /translation="MDVQNSGLQESRQQWRSAVAGVLAKSTRRDPADLPPEPEGLLDS
                     PTYEGFPIRALYTSLDDLPEPPLPGDWPFVRGADARRDVKSGWKVAEAFPVPGQKAVT
                     DGNGAVLVALTEGVSALALRVGTPDGVAAGELDRLLEGVFLDLVPLILDAGADFVAAA
                     DAVLPQLTDMDDEQRARLSIDLGADPLTAPLSRRTTADIADVIAIAQKVTGYAGGVRA
                     ITVDGPALHNIGASASWELAGAVATGVAYLRLLGEAGLSVPDSLRQISFRLAADDDQF
                     MTIAKIRALRRLWARVAEVVGGPDAGAARVHAVTSMPMMAQRDPWVNMLRTTVAAFGA
                     GIGGADTVLVQPFDAAIPGGLPGTAASFARRIAR"
BASE COUNT          393 a          791 c          844 g          333 t
ORIGIN      
        1 acgttcgctg cgatcaaggc gggcaggccg actccggaga tgctggccta ccagtatctg
       61 cagaccctgc cgcagatggc caagggtgag gcgaacaagg tctggctggt gcccagcgac
      121 ttcgggtccg cgctggaggg cttcaccagg atgctgggtg cgcctggcga ggacggtgtc
      181 ttccggtaca cgccatcgcc ggtggacgaa aacctgccca agcccgagga cgactcagcc
      241 gaggtggccg agtggttcaa cacccagacg gatcccgcga tcgctcaggc cgtcgccaag
      301 gccgaggcgg acgcgcgctc ctcatcgggt atcagcgcca ttccggccgc accggaggac
      361 cccccgccgc cgatgcagta tccgccgctg tcacagcagc ggcaggacgg tccgccgcag
      421 tcgccggggc gtcacagcgg ttagccggac tgtcgtcgac gcgatggtgt tgggggtagc
      481 tgcgaatctc gtagatcagg cccgccacac cgagactcgc ggtgaacaac gacacgagga
      541 tcagcagcgg gctgatgtgg tcggtgagcg cgtcaccgag gatgacgacc gccgcggtgc
      601 cgggcaacag gccgaccaga gtcgcccacg tatacggcag cacccgcacg gaggatgcgc
      661 ctgccgcgta gttgagtacc gagaagggta cggcggggat caaccgcatg gacatgatcg
      721 tcggccaacc acgtcggcgc agccgcgcgt cgagggtctt gacccgcgga tggggcacta
      781 ggcggtcgag gcgccagccg gcggcgcgaa ccagcaacag cgcgagcagc gcgctgaccg
      841 cgctcgcgac caccgcgatc gcgataccga gccagggacc gaacagaagg cccgcggcca
      901 gtgtgaatgc ggtgcggggg aatggaaata cggtgacgac gatgtgcgcc gccaggaatg
      961 cgagcggaaa ccagggcccg agcgattgcg cccagtcccg cagctgcatg gcgccgggcc
     1021 ggggcaccag caacagaatt gcgaccagga tcacaattgt ggccgcaatg gccgccaact
     1081 gccgcggggg cacctgcttc gccgtcgaaa tcaccgcact gcgcaccgcg gcgagggtgc
     1141 tgacgacggg cttcacgtct accaagacta cgggtgggtg cggtgatgaa gcgcctcatg
     1201 acgatacgag tcggccgccc ggcgcacgct ggtccgatgg tgatcattta gcctgatgga
     1261 cgtgcagaat tccggcttgc aggaatctcg acagcaatgg cgctcggccg ttgcgggcgt
     1321 gttggcgaaa tcgactcggc gcgacccggc cgatctgccg ccggagcccg agggcctctt
     1381 ggattcgccg acctacgagg gcttcccgat cagggcgctg tacaccagcc tcgacgacct
     1441 gccggaaccg ccgttgcccg gtgactggcc gttcgtccgc ggcgcagacg cgcggcgcga
     1501 cgtcaagtcc ggatggaagg tcgcagaggc gtttccagtc cccggccaga aagccgtcac
     1561 cgatggcaac ggcgccgtct tggtggcctt gaccgagggt gtcagcgcac ttgcgctgcg
     1621 ggtgggcaca cccgacggcg tcgcggcggg cgagctggac cgtctgctcg agggcgtgtt
     1681 cctcgatctg gtgccgctga tcctcgatgc gggtgcggac ttcgtggccg ccgcggatgc
     1741 ggtgctgcca cagctgaccg atatggacga cgagcagcgc gcccggctgt cgatcgacct
     1801 gggcgccgat ccgctcaccg cgccgttgag ccggcgcacc accgccgaca tcgccgacgt
     1861 gatcgcgatc gcacagaagg tcaccggcta cgccggaggc gtgcgggcaa tcacggtcga
     1921 cgggccggct ctgcacaaca tcggcgccag cgcgtcgtgg gagctggccg gtgccgtcgc
     1981 gacgggcgtc gcctacctgc gtcttctcgg cgaggccggc ctcagcgtgc cggactcgtt
     2041 gcggcagatc agcttccggc tcgccgcaga cgacgaccag ttcatgacga tcgccaagat
     2101 ccgcgccttg cgccggctct gggccagggt ggccgaggtg gtcggcggac cggatgccgg
     2161 cgccgcccgc gtgcacgcgg tcacgtcgat gccgatgatg gcgcagcgcg acccgtgggt
     2221 gaacatgctg cgcaccaccg tcgcggcctt cggcgccggt atcggcggcg cggacaccgt
     2281 gctggtgcag ccgttcgacg cggcgatccc cggcggactg cccggcaccg cagcgagttt
     2341 cgcccgccgc atcgcccgca a
//
LOCUS       DASYLX010000316         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2645779,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000316 DASYLX010000000
VERSION     DASYLX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645779"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGP27_07630"
     CDS             complement(<1..417)
                     /locus_tag="VGP27_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851219.1"
                     /translation="MSETLTANASGVTADVPDYPMERDARCPFAPPPQMLKMNETKPL
                     SRVKIWDGSTPWLITGHAVARDLFADSRVSVDDRLDGFPHWNEHMLSTVNKRPRSVFT
                     SDAEEHTRFRRMLSKPFTFKRVEGLRPAIQEVTDECI"
     gene            complement(429..623)
                     /locus_tag="VGP27_07635"
     CDS             complement(429..623)
                     /locus_tag="VGP27_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7851220.1"
                     /translation="MKLTVDQDKCVSSGQCVLNAEDVFDQRDDDGVVLLLNDSPSSEQ
                     ADDVRKAATACPALAIDIEE"
     gene            complement(620..1279)
                     /locus_tag="VGP27_07640"
     CDS             complement(620..1279)
                     /locus_tag="VGP27_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7851221.1"
                     /translation="MTTARAVRADRASGTQEAILKAAERLYAEHGVFAVSNRQVSEAA
                     GQGNNAAVGYHFGTKTDLVRAIEQKHRASIERLLERMVAGIDDSAGLRDWITCMVCSL
                     TEHLAQLGNPTTYARFAAQALTDPAYQKIVVRDALASPSLVQVVEGITRCLPDLPIDV
                     VTERNIMVRNLMMHTCADFERAFAEGEGVPRTTWAAVASGLIDAIVGLWMAPVTEHAL
                     T"
     gene            complement(1439..2278)
                     /locus_tag="VGP27_07645"
     CDS             complement(1439..2278)
                     /locus_tag="VGP27_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851222.1"
                     /translation="MSNELEGKVAIVTGGASGLGEGMARRFAAEGARMLIADVDRDAG
                     LALAKDIGANALFAEADVSQLDQVSGLVTTAVERFGGLHVMVNNAGVSSTMHRSLFDD
                     DLADFEKVMAVNVRGVMAGTRDAARQMRDAGVGGSIINITSIGGILAGGGVLTYRASK
                     AAVIQFTKSAAIELAQYEIRVNAIAPGNIRTAIVRKSAAAEDIEKLEEFEAKIRETMR
                     NDRPLKREGTADDVAEAALYFATDRSRYVTGTVLPVDGGTVAGKVLRTRAAQRDEGRK
                     PNL"
     gene            complement(2279..3118)
                     /locus_tag="VGP27_07650"
     CDS             complement(2279..3118)
                     /locus_tag="VGP27_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7851223.1"
                     /translation="MTQRKTILVDGLTTGYLEAGQGDPVVLLHGGEFGVSADVGWEHN
                     IAALAERYRVLALDMLGFGESAKVIDFNDGRGMRIRHIARFCDAVGVSSAHFVGNSMG
                     AVNLFVDATSESPALRARSLVTICGGGAIQRNEHSAALYDYDATLPGMRRIVEALFFD
                     AAYPRDDAYVQRRYESSIAPGAWEALAAARFRRPGLKPPPLPSSRRAYDRIAVPTLVV
                     EGAGDKLLSDGWAAEIAGQIPHGRSAVVEEAGHCPQIEQPDAVNELLLEFLDDVSAER
                     TAS"
     gene            complement(3115..3702)
                     /locus_tag="VGP27_07655"
     CDS             complement(3115..3702)
                     /locus_tag="VGP27_07655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7851224.1"
                     /translation="MTATTTGGAPLVVGLGGTLRANSSTERALRYCLASVESQGGQTR
                     LFSAEDLDLPMYAPHELERTPKALELVKALRDADAVVVGSPGYHGAISGLVKNALDYI
                     EDLREDPRIYLDNTPWGCISCAYGWQAAVSTLGQLRSIGHALRAWPTPLGVAINSADE
                     IWDSHGRVADATVRNQLDMLATQVLTFARAAWETA"
BASE COUNT          578 a         1234 c         1215 g          675 t
ORIGIN      
        1 gatgcattcg tcggtcacct cctggatggc gggacgcagg ccctcgacgc gcttgaaggt
       61 gaacggcttg gacaacatcc ggcggaaccg ggtgtgctcc tcggcgtcgg aggtgaacac
      121 cgacctcggc cgcttgttga cggtggagag catgtgctcg ttccagtgcg ggaagccgtc
      181 gaggcggtcg tcgacgctga cccgcgagtc ggcgaacaaa tcgcgcgcca ccgcgtgtcc
      241 ggtgataagc cacggcgtgc tgccgtccca gatcttcaca cgcgacaggg gttttgtctc
      301 gttcatcttg agcatctgcg gaggcggcgc gaacgggcag cgcgcgtcgc gctccatcgg
      361 ataatcgggg acgtcagcgg tcacgccgga tgcgttggcg gtcagggttt cggacatagg
      421 cctttggctc attcctcgat gtctatggcc agggccgggc aggccgtggc ggctttgcgg
      481 acgtcgtctg cttgctctga agacgggctg tcgttgagga gaagaaccac gccgtcgtcg
      541 tcacgctggt cgaacacgtc ctccgcattg agcacgcatt gaccggagga cacgcacttg
      601 tcctggtcga cagtcaattt catgtcaggg cgtgctccgt cacgggtgcc atccacaatc
      661 cgacaatcgc atcgatcagc ccggatgcga ctgcggccca tgttgttcgg ggcacacctt
      721 caccctcggc gaatgcccgc tcgaagtcgg cgcaggtatg catcatcagg ttgcgcacca
      781 tgatgttgcg ctcggtgacg acgtcgatcg gcaagtcggg caggcagcgg gtgatgccct
      841 cgacaacctg aaccagcgac ggcgacgcga gcgcgtcgcg gaccacgatc ttctgataag
      901 cggggtcggt cagcgcctgg gcggcgaacc gcgcgtaggt cgtcgggttg ccgagctggg
      961 ccaggtgctc ggtcagcgag cacaccatgc aagtgatcca gtcccgcagc ccggccgagt
     1021 cgtcgattcc ggccaccatc cgctcgagga gccgctcgat cgacgcgcgg tgcttctgtt
     1081 cgatcgcccg gacgaggtcc gtcttggtgc cgaagtggta tcccaccgcg gcgttgttgc
     1141 cctgccctgc cgcctcgctg acctggcgat tagacacggc gaacacaccg tgctccgcgt
     1201 acagccgctc ggccgccttc aggatggcct cttgtgtgcc gctcgcccgg tcggcgcgaa
     1261 cagctctggc agtggtcacg gtcacagtca acccgccccg acggattaag tcaagcgact
     1321 gatttaaagc ttttgcaccg cgagctgggt gcaccctccc tgcaccgcga gcgtgcgtgt
     1381 ctgccttcga cacgccgcgg aaagttgtca ctctgtgcac gctcgtgggg cgatggggtc
     1441 acaggttggg cttgcggcct tcgtcccttt gcgctgcccg cgtgcgcaaa accttgccgg
     1501 cgactgtgcc gccgtcgacg ggtagtacgg ttccggtgac atagcgtgac cggtcggtgg
     1561 cgaagtacaa cgccgcttct gcgacgtcgt ctgccgtgcc ctcgcgtttc agcgggcggt
     1621 cgttgcgcat cgtctcgcga atcttcgcct cgaactcctc gagtttctcg atgtcctccg
     1681 ccgcagccga cttgcgcacg atcgcggtcc ggatattgcc gggtgcgatt gcattgacgc
     1741 gaatctcata ctgcgccagc tcgatcgcgg cggacttcgt gaactggatg acggccgcct
     1801 tcgacgcccg gtaggtcagc actccgccgc cggcgagtat cccgccgatc gaggtgatgt
     1861 tgatgatgga gccgccgacg ccggcgtcgc gcatctgccg cgcggcatcg cgagtgcccg
     1921 ccatcacccc gcgcacattg accgccatca ccttttcgaa gtccgcgagg tcgtcgtcga
     1981 aaaggctgcg gtgcatggtg ctcgacacgc ctgcgttgtt gaccatgacg tgcaggccgc
     2041 cgaaccgctc gactgccgtc gtcaccagac cgctgacctg gtcgagttgc gacacatcgg
     2101 cctcggcgaa cagcgcattg gctccgatgt ccttggccag ggccagaccc gcgtcgcggt
     2161 cgacgtcggc gatcagcatc ctggcccctt cggcggcaaa ccgtcgcgcc atcccctccc
     2221 ccagccccga cgcgccgcct gtgacgatcg cgaccttgcc ttcgagttcg ttgctcacct
     2281 agctcgctgt cctttctgca gacacgtcat cgaggaactc caacaacaac tcgttgacgg
     2341 cgtcgggctg ttcgatctgc gggcagtgac cggcctcttc gacaacggcc gagcggccgt
     2401 gcgggatctg gcctgcgatt tcggccgccc agccgtcgga cagaagtttg tcgcccgcgc
     2461 cctcgaccac cagggtaggc acggcgatgc ggtcataggc tcgtctgctc gacggcagcg
     2521 gcggcggctt caggccgggt cgccgaaatc tggccgccgc caatgcttcc cacgcgccag
     2581 gcgcgatgct ggactcgtag cgccgctgta cgtatgcatc gtcgcgcggg taggccgcgt
     2641 cgaagaagag tgcctcgacg atgcggcgca tgccgggcag ggtggcgtcg tagtcgtaca
     2701 gcgcagccga gtgctcgttg cgctggatcg ccccgccgcc gcagatcgtc accaggctgc
     2761 gcgctcgcag cgcaggggat tccgaggtgg cgtcgacgaa caggttgacc gcgcccatcg
     2821 agttgccgac gaaatgcgcg gaggagacac cgaccgcgtc gcagaaccgg gcgatatggc
     2881 ggatgcgcat gccgcggccg tcgttgaagt cgatcacctt cgccgactcc ccgaagccga
     2941 gcatgtcgag cgccagcacg cggtagcgct cggcgagcgc ggcgatgttg tgctcccacc
     3001 ccacgtccgc gctgacgccg aactcgccgc cgtgcagtag aacgaccgga tctccctgtc
     3061 ccgcttcgag gtaaccagtc gtcaatccat cgacgagtat cgtcttgcgc tgggtcacgc
     3121 cgtttcccaa gcggcacggg cgaacgtgag cacctgggtg gccagcatgt cgagttggtt
     3181 gcgcactgtc gcgtcggcga ctcgcccgtg tgagtcccag atctcgtcgg ccgagttgat
     3241 cgccacgccg agcggcgtcg gccaggcgcg cagggcgtgc ccgatgctgc gcaactggcc
     3301 gagtgtgctg accgccgcct gccacccgta tgcacagctg atgcatcccc acggagtgtt
     3361 gtcgagatag atgcgcgggt cctcgcggag gtcctcgatg tagtcgagcg cattcttcac
     3421 caacccggag atcgcgccgt ggtaaccggg cgaaccgacg acgacggcgt cggcgtcgcg
     3481 cagagccttg accaactcga gcgccttggg agtgcgctcc agttcgtgcg gggcgtacat
     3541 cggcaggtcg aggtcctcgg cactgaacag cctcgtctgt ccgccctgcg attcgacgga
     3601 ggccaggcag tagcgcagcg cccgttccgt cgacgagttg gcccgcagcg ttccgccgag
     3661 cccgacgacg agcggagcac cacccgttgt ggttgcagtc at
//
LOCUS       DASYLX010000317        15617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_123300,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000317 DASYLX010000000
VERSION     DASYLX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123300"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGP27_07660"
     CDS             complement(<1..333)
                     /locus_tag="VGP27_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7851225.1"
                     /translation="MKGLPSMQPTGWFQVAWSADVAEGDVIGLHYFGVDLVAFRDHSG
                     AVHVLNAHCQHLGANLSKGGCVVEDGIQCPFHGWVWNGEGRNVRIPYENRPNRGRRVR
                     SWPVTELNE"
     gene            456..1100
                     /locus_tag="VGP27_07665"
     CDS             456..1100
                     /locus_tag="VGP27_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851226.1"
                     /translation="MSDNGRATERDAIMRAAYRLISEGAAGPTAVSNTSIENILRVAR
                     VNRRVFYRHFACKDDLIIAMQEWAGDLILADLKKAVAAADTPAAAVAAWIEDYLSIGW
                     QEVRFRDALAFMSSEVNGAPGIAAALETTYARHRAVLTDALAAGLADGSLPNARPELD
                     SFAIHAVTVRHLEARIRGRLDTDFREVRDQVVTLMLTGLSAPAMHPDPSGIAAR"
     gene            complement(1176..1337)
                     /locus_tag="VGP27_07670"
     CDS             complement(1176..1337)
                     /locus_tag="VGP27_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851227.1"
                     /translation="MSGFDTSQPIADENSLKDVDTVKKTPDDESAAEKDAVGSTNPDE
                     LGDATPGPR"
     gene            complement(1425..2045)
                     /locus_tag="VGP27_07675"
     CDS             complement(1425..2045)
                     /locus_tag="VGP27_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851228.1"
                     /translation="MTVTSAPVDQLDGGRGERTRAAILDASRRLFLERGYSGTPINAI
                     TEACGISRAGFYTYFKDKREIFNVLGRTAYREALTVIAEWAEADEPFGQNDIRRWVGH
                     YFDYMDHHGAFVLASAHSAPDDDVFRNSRNRMVTRASWKLGQAIAGDGAHSPDVIGVT
                     VMGLLDRAWYTVHRQTVAVDRDEMIAVVTEMIVAMALPVAPRTRGV"
     gene            2194..3423
                     /locus_tag="VGP27_07680"
     CDS             2194..3423
                     /locus_tag="VGP27_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851229.1"
                     /translation="MTQFTDAPIFDADQHMYETPDALTKFLPEEFQPKVQFVQMGKRT
                     RIAILNKITDYMPNPTFERVAAPGAHEKFYSGQNPEGLSMREMSGRGIDTPPGARNPE
                     DRIAELDRQGVDACLNYPTLANLVEHSSAEDPDLTVAIIHALNQWMLEHWGFSHANRI
                     YSTPVLNLGLVDEARRELEYLLDNGAKVALIKPAPVYGYKGWRSPALPEFDPFWRDVE
                     DAGLPIVLHASQPPLQEYIDKWEPPSTSSAFEMSAFKWTALGHREIADMLTSLICHGT
                     LTRFPKLRIASVENGSAWIKPLFDDLQSTYNKMPQNFPEHPHDVFRRNIWVSPFWEGS
                     VADVVATVGWDRVLFGSDWPHPEGLAEPKRYWKYAEGMDTRRTYDFMGDNARRFMGLP
                     IANPDPDAVKPPVLANA"
     gene            3491..4654
                     /locus_tag="VGP27_07685"
     CDS             3491..4654
                     /locus_tag="VGP27_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851230.1"
                     /translation="MSADLNAEEIMLVETVRAFIDRDVRPTVREVEHANEYPHAWIEQ
                     MKRIGIFGLAVPEEYGGSPVSTPCYVEVTQELSRGWMSLAGAMGGHTVVAKLLTLFGT
                     EDQKQKYLPAMATGETRATMALTEPGGGSDLQNITTTALPVGPGGEELAINGSKTWIS
                     NARRSELIALLCKTDVHATPRHQGISVVLVEHGQGLTVSRDLPKLGYKGVESCELSFD
                     DCRVPATAILGGVAGKGFSQMMKGLETGRIQVASRALGVATAALDDALRYAQDRESFG
                     KPIWKHQAVGNYLADMATKLTAARQLTLYAARRYDSGERADMEAGMAKLFASEVAMEI
                     ALNAVRIHGGYGYSTEYDVERYFRDAPLMIVGEGTNEIQRNVIAGQLVTRGGI"
     gene            4671..5510
                     /locus_tag="VGP27_07690"
     CDS             4671..5510
                     /locus_tag="VGP27_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7851231.1"
                     /translation="MTEAPPQSRDLGVVRYERDGAIARIVLDWPDRANAQSSEMVSQV
                     DSCLDEARQDYGVKVVIVKGAGKGFCAGHVIAPEAYPEFAESQAHMGSNYQGSKELFL
                     WPMLRYWEFPKPMIAQVHGYAIGGGTYWALLPELTIASDDAYFQMPLVPGLGFAGGET
                     MIEPYVFMNYKRAAEYLYTAQTLTAEEALRMGLVNRVVPREDLEPVTEALAAKIARAP
                     LSTLMATKTMLVRAWEQMGMRQHLQLSTDVMSVLENTADAQALRAELLARGRLPRDYA
                     ADS"
     gene            complement(5507..6496)
                     /locus_tag="VGP27_07695"
     CDS             complement(5507..6496)
                     /locus_tag="VGP27_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7851232.1"
                     /translation="MAGHVKLVIGASGFLGSHVTRQLVGRGERVRVLLRRTSSTVAID
                     DLDVERHYGDVFDDAALRDAMAGCDDVYYCVVDARAWLRDPAPLFRTNVQGLRHALDA
                     AVDADLRRFVFTSTIGTIALSENGHPVTEDEPFNWADKGGGYISSRVAAENLVLQYVR
                     ERGLPAVALCVANTYGPGDFQPTPHGSLVAAAAKGKMPVYVKGMSMEVVGIEDAARAL
                     ILAAETGRVGERYIISERFLSAHELYGTAAEAGGARPPRIGIPLKLMYALGFGGDVAA
                     KVLRRDMLVSTLSVRLMHIMSPMDHGKAERELGWQPEPIHDSIRRAVAFYRKR"
     gene            complement(6512..7912)
                     /locus_tag="VGP27_07700"
     CDS             complement(6512..7912)
                     /locus_tag="VGP27_07700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7851233.1"
                     /translation="MKRLGGWDAVLLYNETPNLHQHTMKVAVVDASDCDDFGFDRFRQ
                     TLARRLHLLEPLRYQLAEIPGKLHHPMWLENCDVDLDYHVRRMQIRPPGGRREFDAAI
                     GEVACTPLDRSRPLWEFHFVEGMPDNRFAIIGKVHHALADGVASANLMARALDLPGSS
                     QSERDDYRRDPCPSNAQLLRAALRDHAALLRELPRLLSDTVKGIRRVRRESRSAPDLA
                     RNFHPPATFLNHVVTPGRTFASATLDLAEVKAIGKQLGVTINDMVLAISTGALRTLLL
                     KHDGHADEPLLCGVPLSIDTSPERISGNAIGTIVVSLPVHVTDPLDWVRLARVGAMIG
                     KENARLLGHELVSRWSSYAPPALTEWGFGRIAVSGPNKLVNAAISNVPGPRDFGRIAC
                     APMVELYSVGPLTFGVGVNITVWSYVDQLNISVLADDVTFADPHEMTDAMVEAFGIIR
                     QAAEIPTDAARRPRAL"
     gene            8029..8835
                     /locus_tag="VGP27_07705"
     CDS             8029..8835
                     /locus_tag="VGP27_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7851234.1"
                     /translation="MTSSDIEPSAITKWDPGLTERLMRLIRPVIKGWHRAEVRGLDEF
                     PPAGALVVANHSGGLFAMDVPVFITDFYARFGYDRPVYTLTHDLMFSTPAGGLLRRTG
                     FIPASHENADEALRAGGVVVVFPGGDYDVYRPTMSANKIDFGGRTGYVRAALNAGVPI
                     VPTVSIGGQENQLFLSRGEWLSKATRLDKLLRAKILPISFGFPFGLSAVVPVNLPLPT
                     KIVTQVLPPIDIVAEFGEDPDIHEVDAHVRHVMQQALDTLAAARRLPVIG"
     gene            8841..10481
                     /gene="fadD12"
                     /locus_tag="VGP27_07710"
     CDS             8841..10481
                     /gene="fadD12"
                     /locus_tag="VGP27_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase FadD12"
                     /protein_id="HEV7851235.1"
                     /translation="MAILERFNQTAGIVSTLARAGVIAPMRPDRYVRIAAAVRREGMT
                     VASGFAAAAQRCPDRPALIDELGTLTWRELDQHSDALAAALQGLPGKVDVVGIMARNH
                     RGFVESLIAATRIGADVLLLNTSFAGPALAEVVAREQVDAVIYDEEFGPAVSQAIKDR
                     PKATKIVAWTDDPDAHALTVAGLIDGHMRQRPSPSARTSKLILLTSGTTGSPKGAKHS
                     GGGPAELKSILDRTPWRAEETAVIVAPMFHAWGFGQLVFAAFMACTVVTRRKFDPEAT
                     LALIDEHRATGLCVVPVMFDRIVDLPDDVLSRYSGRTLRFAAASGSRMRPDVVIEFMD
                     RFGDVIYNNYNATEAGMIATATPEDLRATPDTAGRPAAGTDIRILDSEFREQPPGEVG
                     TIYVRNSSQFDGYTSGATKDFHDGYMSSGDVGYVDADGRLFVVGRDDEMIVSGGENIY
                     PIEVEKTLAAHPGVSEAAVLGVDDEKYGQRLAAFVVLSEGASATPDTLKEHVRENLAN
                     YKVPRTITVLDDLPRSSTGKIVRRELQALVETDADASG"
     gene            complement(10482..10784)
                     /locus_tag="VGP27_07715"
     CDS             complement(10482..10784)
                     /locus_tag="VGP27_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851236.1"
                     /translation="MIKNGTRLKSQVCDAQVIVVKAADSLDDLRCGGTPMLPLDAENA
                     SDASLDPDFSGGAVMGKRYVDEGGAEVLVTKAGAGTLSIGSTALTLKEAKPLPASD"
     gene            complement(10796..12340)
                     /locus_tag="VGP27_07720"
     CDS             complement(10796..12340)
                     /locus_tag="VGP27_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEV7851237.1"
                     /translation="MSISLLLEMAVSEDPARVAVVDGARRLTIGELSELADGAAGVIT
                     ASGVEHVAYVGSGGIMAPLLIFGSARAGRPYTPINYRLSPDGIQELIGRLPNPLVVVD
                     ARYRDMVGDVGKRGALPSQAGGPPACGGVMDSGDFLAAARTAEPAAEFADPDEVAIVL
                     FTSGTTSKPKAVELTHGNLTSYITGTVDFGSADTGDAALICVPPYHIAGVNAAMSNLY
                     AGRKMVYLANFEAKEWVRLVADEKVTTATVVPTMLDRIVTALEQEPIGLPALRNLAYG
                     GSKVGLPLVRKALDLLPHVGFVNAYGLTETSSTIAVLTPDDHREAHGNPDETVAKRLG
                     SVGRPVPGIEVQIRDENGGVLPPGKPGELHVRGEQVSGTYTGIGSVLDDEGWFPTKDI
                     ATLDEAGYLFITGRSDDTIIRGGENIAPAELEDVLIEHPHVHEVAVVGVEDPQWGQAI
                     VAVVVPAQGADPTPEELREYARKFLRGSRTPDRVVFRDELPTNATGKLLRREIVDSLR
                     DAKAEK"
     gene            12487..13641
                     /locus_tag="VGP27_07725"
     CDS             12487..13641
                     /locus_tag="VGP27_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7851238.1"
                     /translation="MSSTPDIAIIGVGLHPFGRYEDRSALEMGAVAISRALRDAGVSF
                     SDVGSVYAGSLEVANPEAITGLVGMTGVPARATLSGCATGNSLLTLAARDVALGEAEI
                     AVGVGLDKHPRGAFGADPSVSGLPQWYGDQGMFLTTHYFGTKIMRYMHDHGITDQTLA
                     RVAAKNFDNGAKNPNAWRRKPMTEEAILASPVVNAPLRQYMYCNPNEGAAAVVVCRAD
                     AAKKYTDTPIYLRSTALRSRREGAYELLRTSIELPIVPGTTAEAAKAAYEAAGIGPED
                     IDVAQLQDTDAGSEVIHMAETMLCKDGEQEALLADGATQIGGRLPINTDGGLLANGEP
                     VGASGLRQVFELVHQLRGTAGDRQVPSDPRVALAQLYGAPGTAAVAILSR"
     gene            13665..14885
                     /locus_tag="VGP27_07730"
     CDS             13665..14885
                     /locus_tag="VGP27_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851239.1"
                     /translation="MTDTQAEARRRAEVDLDHHSPEFREDPYGRFREMRESGCPVAHS
                     NHYEGFWALADYASVFEAARDDDLFNSFPSVGVPASELPLPILPIESDPPETQELREV
                     TLKRFSPGAAERFRESAIEMTDEAIDEFIERGECDLVGALTTPLPARLILRLLKFDES
                     RHMDWVSWVHSTVHDRAHDPEKAGMAGMDMFGEIVKHMEERRAEGLGDDLFSDILRGT
                     LNGKPLDDGQITMYTVLMMLGGMDTTSGFTGNVLSQLCRDPGLRQQFIDDPGLVKKGT
                     DELLRLYTPTLGLARTVSRDAEFHGQPLCKGDRAILLWAAANRDPAMFDDPDKLDLSR
                     PNAKKQMAFGVGMHRCLGSHYAKMMFDVMVTQILKRLPDFELAGEPKMFEDAGEVYAV
                     RELPVKFTPGPRKG"
     gene            14889..>15617
                     /locus_tag="VGP27_07735"
     CDS             14889..>15617
                     /locus_tag="VGP27_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851240.1"
                     /translation="MASVAVGDVEIDYADTGSGPVVVFVHGAYVTGRLWQDVVDRLSD
                     NHRCIAPTLPFGAQTEPVGAHVDLGVVASGTLIVGLLEALDLSDVTLVANDTGGGIVL
                     ASLGNPTLDWDRVSKLVFTNCDSFEHFPPSAFAPIVRLCALSQALGAIVLKGLTTSAG
                     LKVFTSAVTRHGIDAARRPAIFGGFLTSATVRREAARFTADLKPSHTMAAVEALKRWD
                     KPVLMAWGTDDKMFPPAHAHRLAET"
BASE COUNT         2783 a         5158 c         5014 g         2662 t
ORIGIN      
        1 ctcattgagt tcggtcaccg gccaggaccg cacccgacgt ccccgatttg gccggttctc
       61 gtatgggatc cgcacattgc ggccttcccc attccacacc cagccgtgga aggggcactg
      121 aatgccgtcc tcgacgacgc acccaccctt ggacaggttg gccccaaggt gttgacaatg
      181 cgcgttgagc acgtgaacgg cgccgctgtg gtcacggaag gcgacgaggt cgacgccgaa
      241 atagtgcaaa ccgatcacgt cgccctcggc gacgtcggcg ctccaggcga cctgaaacca
      301 tccggtcggc tgcatgctgg gcaggccctt cacgttttac tcctcacaat tgcagcgcat
      361 caccactgat atcagcacac agtatcggga atggcgttcc cagacaatgt ggtgatactt
      421 ttgggaaaag tattcccacc gaacggagtg cgcgaatgag cgataatggt cgcgcgactg
      481 agcgcgatgc gatcatgcgc gccgcctatc ggttgatcag cgaaggcgcc gccgggccga
      541 ccgcagtctc gaacacgtcc atcgagaaca tcctgcgggt cgctcgggtg aaccgacgcg
      601 tcttctaccg gcacttcgcg tgcaaggacg acctgatcat cgcgatgcag gagtgggcgg
      661 gcgacctgat cctggccgat ctcaaaaagg cggtcgcggc cgccgacacg cccgctgcgg
      721 ccgtcgccgc ctggatagag gactacctga gtatcggttg gcaggaagtg cgattccggg
      781 acgccttggc tttcatgtct tcggaggtca acggcgcgcc gggcatcgca gcagcgctgg
      841 agaccaccta tgccaggcac cgagcggtgc tgacagatgc actggccgcg ggcttggccg
      901 acggcagttt gccgaatgcg cgcccagagc tcgactcctt cgcaatccac gccgtcaccg
      961 tgcgtcatct ggaggcacgc atccgcggtc gccttgacac cgattttcgt gaggtgcgcg
     1021 atcaggtcgt cacgctgatg ctgaccggac tctccgctcc cgccatgcac cccgacccgt
     1081 ccggaattgc cgcccgttga ggcggtcggc gatgggcggg aaagccattc ccacgactaa
     1141 aaagaattca tttaggtata aggctgacgc ccgtatcagc gggggccggg cgtagcgtct
     1201 cccagctcgt cggggttggt actgccgacg gcgtccttct ctgcggcgga ctcgtcgtcg
     1261 ggcgtcttct ttaccgtgtc gacatccttc aacgagttct cgtcggcgat gggttgactc
     1321 gtgtcgaaac ctgacattcg ttgctccttc agtgccgaaa tgagccggcg cgaggctgag
     1381 ccgacggtgt gtacttccga tctattaccc gcaccgccag tcggttagac gccccgggtt
     1441 cgtggcgcaa cgggcaacgc catggccaca atcatctccg tgaccaccgc gatcatctcg
     1501 tcgcggtcca cggcgactgt ctgccggtgc acggtgtacc aggcccggtc cagcaggccc
     1561 atcaccgtga ctccgatgac gtccggggag tgcgcgccgt cgccggcgat ggcctggccc
     1621 aatttccacg acgcacgcgt caccatgcgg ttgcgcgagt ttcggaaaac gtcgtcgtcg
     1681 ggcgccgagt gcgccgacgc aagcacgaac gctccgtggt ggtccatgta gtcgaaatag
     1741 tggccgaccc agcgccggat atcgttttgc ccgaacggtt catcggcctc ggcccactcg
     1801 gcgatcacgg tgagggcttc gcgatatgcc gtcctgccaa ggacgttgaa gatctcccgc
     1861 ttgtccttga agtaggtgta aaaacctgcc cgcgagatgc cacacgcctc ggtgatcgcg
     1921 ttgatcggcg tcccgctgta gccgcgctcg aggaaaagcc tgcggctggc gtccaggatg
     1981 gccgcgcggg tccgctcgcc ccgaccgccg tccagttggt cgacgggcgc cgaggtcacg
     2041 gtcatggcgc gaccatatat gacggtattg tcaagcgcaa tcaccgcagt taaacaagct
     2101 gttcacagga atctagatga gaacttgaca caagtgtcaa gttacgcgaa gctcgtagcc
     2161 aagcccgatg gagagcagtg ggaggccaca ccaatgacgc agttcaccga cgcaccgatc
     2221 tttgatgccg accagcacat gtacgagacc cccgatgcac tgaccaaatt cctgcccgag
     2281 gaatttcagc cgaaggtgca gttcgtccag atgggcaagc gcacccggat cgcgatcctg
     2341 aacaagatca ccgattacat gccgaacccg acgttcgagc gggtcgccgc gcccggcgcg
     2401 cacgagaagt tctactccgg ccagaacccc gagggcctgt cgatgcggga gatgtccggt
     2461 cgcggcatcg acacgccgcc cggcgcgcgc aaccccgagg accggatcgc cgaactcgac
     2521 cgccagggcg tcgacgcgtg cctgaactac ccgacgctgg ccaaccttgt ggagcactcc
     2581 tccgccgagg accccgacct gaccgtggcg atcatccacg cactgaacca gtggatgctc
     2641 gagcattggg ggttcagcca cgcaaatcgg atctacagca ctccggtgct gaacctcggc
     2701 ctggtggacg aggcgcgtcg cgagctcgag tacctgctcg acaacggcgc caaggtcgca
     2761 ctgatcaagc cggccccggt gtacggctac aaggggtggc gctcgcctgc gctgcccgag
     2821 ttcgacccgt tctggcgcga cgtcgaggac gccggcctgc cgatcgtgct gcacgccagc
     2881 cagccgccgc tgcaggagta catcgacaag tgggagccgc cgtcgaccag cagcgcattc
     2941 gagatgtcgg cgttcaagtg gacggcgctg gggcaccgcg agatcgctga catgctgacc
     3001 agcttgatct gccacggcac gttgacccga ttcccgaagc tgcgcatcgc cagcgtcgag
     3061 aacggcagcg cgtggatcaa gccgctgttc gacgatctgc agtcgacgta caacaagatg
     3121 ccccagaact ttcccgagca tccgcacgac gtgttccgcc gcaacatctg ggtcagtccg
     3181 ttctgggagg gctcggtggc cgacgtcgtg gcgaccgtcg gatgggacag ggtgctgttc
     3241 ggctcggact ggccgcatcc cgaaggtttg gctgagccca agcgttattg gaagtacgcc
     3301 gagggcatgg acacgcggcg cacctatgac ttcatgggcg acaacgcacg ccgcttcatg
     3361 gggctgccga tcgccaatcc cgatccggac gccgtcaagc cgcctgtact ggcgaacgcc
     3421 tgatcgcgcc gtccaagact tttcgcgacg tcggcgttgg cgccggcgcc gacctcggta
     3481 gggatgccac atgagtgcgg atttgaacgc cgaagaaata atgctcgtcg agaccgtgcg
     3541 cgccttcatc gatcgcgacg tccggccgac ggtccgcgag gtggagcacg ccaacgaata
     3601 cccgcacgcg tggatcgagc agatgaagcg catcggcatc ttcggcctcg ccgttcccga
     3661 ggagtacggc ggctcccccg tctcgacgcc ttgctacgtc gaggtgacac aagagttgtc
     3721 tcgcggctgg atgagcctgg ccggcgcgat gggcgggcac acggtcgtcg ccaaactcct
     3781 cacactcttc ggtaccgaag accagaagca gaaatacctg cctgcgatgg cgaccggtga
     3841 gacgcgggcg acgatggcac tcaccgaacc tggcggcggg tcggatctgc agaacatcac
     3901 caccaccgcg ctgcccgtcg gcccaggcgg tgaggaactg gcgatcaacg gttccaagac
     3961 ctggatcagc aatgcacggc gctcggagtt gatcgctctt ttatgtaaga ccgacgtgca
     4021 cgccacccca cgacatcagg gcatttcggt ggtgttggtc gaacacggcc agggcctcac
     4081 cgtctcgcgc gaccttccga aactcggtta caagggcgtc gaatcctgcg aactgtcgtt
     4141 cgacgactgc cgggtgcctg ccacggccat tctcggcggc gtcgcaggca aggggttctc
     4201 gcagatgatg aaaggccttg agacaggccg cattcaggtg gcctcacgcg cgctgggtgt
     4261 ggcaacggcc gcgctcgatg acgcgttgcg ctacgcgcag gatcgtgaaa gctttggcaa
     4321 gccgatttgg aagcatcagg ccgtgggcaa ttatctcgcc gacatggcca ccaaactcac
     4381 tgccgcgcga caactcacgt tgtacgccgc gcggcgctac gacagcggcg aacgcgcaga
     4441 catggaagcg ggtatggcga agctcttcgc gtctgaggtt gcgatggaaa tcgccttgaa
     4501 tgccgtccgg atccatggtg gatacggcta ctccaccgaa tacgatgtcg aacggtactt
     4561 ccgcgatgca cccctgatga tcgtcggcga aggcaccaac gaaatccaac gcaacgtgat
     4621 cgccgggcag ctcgtgacgc gcggaggcat ctgagggatt cgaggcaatc gtgaccgaag
     4681 ctccgcccca gagtagagat ctcggcgttg tgcgctacga gcgtgacggc gcgatcgccc
     4741 gcattgtcct cgattggccg gaccgggcca acgcacagtc ctcggagatg gtgagccagg
     4801 tggactcgtg cctcgacgag gcccggcagg actacggggt caaggtggtc atcgtcaagg
     4861 gcgcgggcaa gggcttctgc gccggacatg tcatcgcccc cgaggcctat cccgagttcg
     4921 ccgagtcgca ggctcacatg ggcagcaact accagggcag caaggagttg ttcctctggc
     4981 ccatgcttcg gtactgggag ttcccgaagc cgatgatcgc gcaggtgcac ggctacgcga
     5041 tcggcggtgg cacgtattgg gcgctgctgc ccgaactcac gatcgcctcc gacgacgcct
     5101 actttcagat gccgttggtg cccggcctcg gcttcgcggg tggggagacg atgatcgagc
     5161 cgtacgtgtt catgaactac aagcgcgccg ccgaatacct ttacaccgca cagactctga
     5221 ccgccgagga agcgttacgg atgggcctgg tcaaccgcgt cgtcccgcgc gaggatctcg
     5281 agccggtgac cgaagcgttg gcggccaaga tcgcccgcgc tccgctgtcc accctgatgg
     5341 cgacgaagac catgctggtg cgcgcgtggg agcagatggg catgcgccag caccttcagt
     5401 tgtcgacgga cgtgatgtcg gtgctggaga acaccgccga cgcgcaggcg ctacgtgccg
     5461 agcttctggc acgcggccga cttccccgcg actacgcggc cgacagctag cgcttccgat
     5521 agaacgccac cgcccggcgg atcgaatcgt gaatcggctc cggctgccat ccgagctccc
     5581 gctcggcctt gccgtggtcc atcggcgaca tgatgtgcat gagccgcacc gacagggtgg
     5641 acaccagcat gtcgcgtcgg agcaccttcg cggcgacgtc tccgccgaat cccaatgcgt
     5701 acatgagctt cagcggaatc ccgatccgag gcggcctggc tcccccggcc tcggccgccg
     5761 tcccgtagag ctcgtgagcg ctgaggaatc gctccgagat gatgtatcgc tcgccgacgc
     5821 ggcccgtctc ggccgccagg atcagcgcgc gggccgcgtc ctcgatgccg accacttcca
     5881 tcgacatccc cttgacgtac acgggcatct tgcccttcgc cgcagcagcc actaacgaac
     5941 cgtgcggcgt cggttgaaag tcaccggggc cataggtgtt cgcaacgcac aacgcgaccg
     6001 cgggcagtcc ccgctcccgg acgtattgca ataccaagtt ctcggcggcc acccgggaac
     6061 tgatgtagcc gccgcccttg tccgcccagt tgaagggttc gtcctcggtg acggggtgtc
     6121 cgttctcgct gagcgcgatg gtgccgatgg tgctggtgaa gacgaagcgc cgcaggtccg
     6181 catccacggc cgcgtcaagc gcgtggcgca ggccctggac gttcgtacga aacaacggag
     6241 cggggtcgcg cagccacgcc ctggcgtcca cgacgcagta gtacacgtcg tcgcagccgg
     6301 ccatcgcgtc gcgcagcgcc gcatcgtcga agacatcgcc gtaatggcgc tcgacgtcga
     6361 ggtcgtcgat ggcgaccgtc gaactggtgc ggcgcagcag gactcgcacc cgctcgcccc
     6421 ggcccaccag ctgtcgtgtg acgtgcgaac cgaggaatcc gctcgcgccg atcaccaact
     6481 tcacgtgacc tgccatcgtg gtcgtatgcg actagagagc gcgcggccgg cgcgccgcgt
     6541 ccgtcggtat ctcggccgct tgccggatga tgccgaacgc ttcgaccatc gcgtcagtca
     6601 tctcgtgcgg atccgcaaag gtcacgtcgt cggcgagtac cgagatgttg agctggtcga
     6661 cgtagctcca gaccgtgatg ttcactccga ccccgaacgt gagcgggcct accgagtaga
     6721 gctcgaccat cggggcgcat gcgatccggc cgaagtcgcg cggcccgggc acgttggaga
     6781 tggcagcgtt gaccagtttg ttgggtccac tgaccgcgat gcggccaaag ccccactccg
     6841 tcaaggccgg tggagcgtag ctcgaccagc ggctcactag ctcgtggccg agcaggcggg
     6901 cgttctcctt gccgatcatg gcgccgaccc gggccagacg cacccagtcc agcggatcgg
     6961 tgacgtgcac cggtagcgac accacgatgg tcccgatcgc gttccccgat atccgctccg
     7021 gcgacgtgtc gatgctcaac ggcactccgc acagcagcgg ttcgtcggcg tggccgtcgt
     7081 gcttgagcag gagtgtgcga agtgcccccg tgctgatggc cagcaccatg tcgttgatcg
     7141 tcacgccgag ttgcttgccg atcgccttga cttcggcgag gtcgagcgtg gcgctggcga
     7201 aggtgcggcc cggcgtgacg acgtggttga ggaaggtcgc aggcggatgg aagttgcgag
     7261 caaggtcggg tgcggacctg gattcgcggc gcacccgccg gatgcccttg acggtgtccg
     7321 agagcaggcg cggaagctcc ctgagcaacg ccgcgtggtc acgcagcgcg gcacgtagca
     7381 gttgcgcatt ggacgggcag gggtcccgcc ggtaatcgtc tcgttcggac tgtgaggaac
     7441 cgggcaggtc cagcgcacgc gccatcaggt tggccgacgc cacgccgtct gcgagcgcgt
     7501 ggtgaacctt cccgatgatg gcgaaccggt tgtcgggcat gccctcgacg aaatggaact
     7561 cccacagcgg cctgcttcgg tccagcggag tgcaggccac ctcgccgatc gccgcatcga
     7621 actcccgacg accccctggc ggcctgatct gcatccgccg aacgtggtaa tcgagatcga
     7681 catcgcagtt ctccaaccac atcggatgat ggagcttgcc cggaatttcg gcgagctggt
     7741 accgcaacgg ttcgaggaga tgcagccggc gcgcgagcgt ctgacgaaag cggtcgaacc
     7801 cgaaatcgtc gcagtccgac gcgtcgacga ccgcgacttt catcgtgtgc tggtgcaggt
     7861 tgggcgtctc gttgtagagc aggaccgcgt cccagcctcc gagcctcttc aacacccctc
     7921 catcgccgtg aatcggtcat cgatgtggcg tctccacggt aaacgggtac ccggcgacgg
     7981 cgaggggtaa taaattaaat atctaacgat ttctagacag ggggccaggt gacttcgtct
     8041 gacatcgagc cgtccgccat cacaaaatgg gatccgggtc tcaccgaacg gttgatgcgc
     8101 ctgatccggc cggtgatcaa gggctggcac cgtgccgagg tgcggggact cgacgaattt
     8161 ccgccagccg gcgcactggt ggtggccaac cactcgggtg ggttgttcgc gatggacgtg
     8221 cccgtcttca tcaccgactt ctacgccagg ttcggctacg accggcccgt ctacaccctg
     8281 acccacgacc tcatgttctc cactccggca ggtggcctcc tcagaaggac tggtttcatt
     8341 ccggcaagcc acgagaacgc cgacgaggcc ctacgcgcag gtggcgtcgt cgtggtcttt
     8401 ccgggcggtg actacgacgt ctaccggccg acgatgtcgg cgaacaagat cgacttcggc
     8461 ggccgcaccg ggtacgttcg cgcggccctg aacgccggcg tgccaatcgt tccgacggtg
     8521 tcgatcggcg gccaggagaa ccagctgttt ctgtcccgtg gcgagtggct gtcgaaggcg
     8581 acgcgcctgg acaaattgct gcgggccaag attctgccga tctcgttcgg ctttccgttc
     8641 gggctttcgg cagtcgttcc ggtgaacttg ccgctaccga caaagatcgt cactcaggtg
     8701 ctgccgccca tcgacatcgt cgcggagttc ggcgaggacc ccgacatcca cgaggtggac
     8761 gcccacgtcc gtcacgtcat gcagcaggcg ctcgacacgc tcgccgccgc acgacgactc
     8821 cctgtgatcg gttgacacca atggccatcc tcgaacgctt caaccagacc gccggcatcg
     8881 tgagcaccct cgctcgcgcg ggcgtcatcg ccccgatgcg gcccgacaga tacgtccgga
     8941 tagctgcggc agtacgacgc gagggcatga ccgtcgcctc gggttttgcg gcagccgccc
     9001 agcgctgccc cgaccggccc gcgttgatcg atgaactcgg cacgctgacc tggcgtgagc
     9061 tcgaccagca cagcgacgca ttggccgcgg ccctgcaggg cctgcccggc aaggtcgacg
     9121 tcgtcggaat catggcccgc aatcaccgcg ggtttgtcga gtcgttgatc gccgccacca
     9181 gaatcggtgc cgacgtgttg ctgctgaaca cgtcgttcgc cggtccggca ctggccgagg
     9241 tggtggcccg cgagcaggtc gacgccgtca tctacgacga ggagttcggt ccggccgttt
     9301 cccaggcaat caaggaccgt ccgaaggcaa ccaagatcgt ggcgtggacg gatgatcccg
     9361 acgcgcacgc cctcaccgtc gccggcttga tcgacgggca catgcgtcaa cgcccctcgc
     9421 cgtccgcgcg gacgtcgaag ctgatcctgc tgacgtcggg taccaccggc tcgcccaagg
     9481 gcgccaagca ttccggcggt ggacccgcag agctcaagtc gatcctcgac cggacgccat
     9541 ggcgggccga ggaaacggcc gtgatcgtcg cgccgatgtt ccacgcctgg ggattcgggc
     9601 aactggtatt cgcggccttc atggcgtgca ccgttgtcac ccggcgcaag ttcgaccccg
     9661 aggccaccct cgccctcatc gacgaacacc gcgccaccgg actctgcgtg gtgccggtca
     9721 tgttcgaccg catcgtcgac ctgcccgacg acgtactctc ccgctacagc ggccgcacac
     9781 tgcggttcgc ggccgcttcg ggatcacgaa tgcgaccgga cgtcgtcatc gagttcatgg
     9841 atcggttcgg cgacgtcatc tacaacaatt acaacgccac cgaggcaggc atgatcgcga
     9901 cggccacccc cgaggacctc cgtgcgacgc ccgacaccgc gggcagaccc gcagcgggaa
     9961 cagacattcg catcctcgac agcgagttcc gcgaacaacc ccctggtgaa gtcggcacca
    10021 tctacgtccg gaactccagc cagttcgatg gctatacgtc gggcgccacc aaggactttc
    10081 acgatggcta catgagttcc ggcgacgtcg ggtacgtcga cgcggacggc cgcttgttcg
    10141 tcgtcggccg cgacgacgag atgatcgtgt ccggtggcga gaacatctac ccgatcgagg
    10201 tggagaagac gctggctgcg catccagggg tgtccgaggc ggctgtgctc ggcgtcgacg
    10261 acgaaaaata cggccagcgg ctggcggcgt tcgtcgtgct ctccgaaggt gcctccgcca
    10321 cgccggacac actcaaggag catgtgcgcg agaacctcgc caactacaaa gtgccgcgga
    10381 cgatcacagt gctcgacgat ctgccccgca gcagcacggg caagattgtc cgtcgcgagc
    10441 tacaggcact cgtcgagaca gacgccgacg ccagtggctg atcagtcgct ggcgggcagc
    10501 ggcttggctt ctttcagcgt caacgcggtc gacccgatgc tcaacgtgcc ggctcccgct
    10561 ttggtcacca gcacctctgc gccgccctcg tcgacgtacc gtttgcccat cacagcgccg
    10621 ccggagaagt cgggatccag gctggcatcc gaggcgttct cggcgtccag aggcaacatg
    10681 ggcgtgccgc cacaccgcag atcgtcgagg ctgtcggccg ccttcacgac gatgacctgg
    10741 gcatcgcaca cctggctctt gagcctagtg ccgttcttga tcatggtgcg ttcctctact
    10801 tttcggcttt cgcatcgcga aggctgtcga cgatctcccg ccgcagcagt ttgcctgttg
    10861 cattcgtcgg caactcgtca cgaaacacga cgcggtccgg ggtccgcgat cctcggagga
    10921 atttccgagc gtactcgcgc agttcctcgg gagttggatc ggcgccctgg gcgggaacca
    10981 ccacggcgac gatcgcctga ccccactgcg gatcctccac accgaccacg gccacctcgt
    11041 gcacatgtgg atgctcgatg agcacatcct cgagctcggc aggcgcgatg ttctccccac
    11101 cgcggatgat ggtgtcgtct gagcgcccgg tgatgaacag gtagcccgcc tcgtcgagcg
    11161 tcgcgatgtc cttggtggga aaccagcctt cgtcgtccag caccgatccg atgccggtgt
    11221 atgtgccgga aacctgctcg ccgcgcacgt gaagctcacc tggcttgccc ggcggcagga
    11281 caccgccgtt ctcgtcgcgg atctgcacct cgatcccggg caccggccgc ccgaccgagc
    11341 cgaggcgctt ggcgacggtc tcgtcgggat tgccgtgcgc ctcgcggtgg tcgtcggggg
    11401 tcagcactgc gatggtcgag ctggtttcgg tcaggccgta ggcgttcacg aacccgacgt
    11461 gcggcaacag gtccaatgcc ttccgcacca acggaagccc caccttcgac ccgccgtacg
    11521 ccaggttgcg cagcgcgggc agtccaatcg gctcctgctc gagcgcggtg acgatgcggt
    11581 cgagcatggt cggcacgacg gtcgcggtgg tcaccttttc gtctgcgacg aggcgcaccc
    11641 actcctttgc ctcgaaattg gccaggtaca ccatttttcg gccggcatag aggttcgaca
    11701 tcgccgcgtt gacaccggcg atgtgatacg ggggcacgca gatcagtgca gcgtcgccgg
    11761 tatcggccga accgaagtcg acggtgccgg tgatgtagct ggtcaggttc ccgtgcgtca
    11821 attcgaccgc tttaggtttc gacgtcgtgc ccgaagtgaa caggacgatc gccacctcat
    11881 cgggatcagc gaactccgcg gccggttctg cagtgcgcgc cgcggccagg aaatcgccgg
    11941 aatccatcac tcccccgcaa gcgggtgggc ccccagcttg cgaggggagt gccccgcgct
    12001 tgccgacgtc gccgaccatg tcccggtagc gagcgtcgac gacgaccagg ggattcggca
    12061 gccgtccaat cagctcctgg atgccgtcgg gactcaaccg atagttgata ggcgtgtacg
    12121 gccgcccggc gcgcgctgag ccgaagatca gcagcggtgc catgattccg cccgagccga
    12181 catatgcgac atgctcgacg cccgacgcgg tgatcacccc ggccgcgccg tcggccagtt
    12241 cgctgagctc tccgatggtc aaccggcggg caccgtccac cacggcaacc cgggcggggt
    12301 cttcggagac cgccatctcc agcaacaacg aaatgctcaa cgcctcagcc ttcactcgcg
    12361 agtaggccga ttgtagactc cggcctgaga atcaggtata tagttataga cttacggacg
    12421 tcaaatcacc gcgcacctgg ccgttgaccg gctccctgat cgcgcacaga tgccagaagg
    12481 agcgcaatga gcagcacgcc cgatatcgcg atcatcggtg tcggtttgca cccgttcggg
    12541 cgatacgagg accggtcggc gctggaaatg ggcgccgtgg cgatcagcag ggcattgcgc
    12601 gatgccgggg tgagcttctc cgacgtgggc agcgtctatg cgggcagcct ggaagtggcc
    12661 aacccggagg cgatcaccgg cctggtcggc atgacgggcg tgcccgcgcg tgccacgctg
    12721 agcggttgcg cgacgggcaa ctcgctgctg acgttggcag cccgagacgt agcgctcggc
    12781 gaggcggaga tcgcggtcgg ggtcgggctg gacaagcatc cgcgcggcgc attcggtgcc
    12841 gatccgtcgg tgtcgggctt gccgcagtgg tacggcgacc agggcatgtt cctgaccact
    12901 cactacttcg gcaccaagat catgcggtac atgcacgacc acggcatcac cgaccagacg
    12961 ctggcccgtg tcgccgccaa gaacttcgac aacggcgcca agaaccccaa cgcctggcgc
    13021 cgcaagccga tgaccgagga ggccatcctg gcttcgccgg tcgtcaacgc gccactgcgg
    13081 cagtacatgt actgcaatcc caacgaaggc gccgcggcgg tggtggtctg ccgggccgat
    13141 gcggccaaga agtacaccga caccccgatc tatctacgct cgaccgcgct gcgcagccgc
    13201 cgcgagggcg cctacgaact gctgcgcacc tccatcgagc tacccatcgt tcccggcacc
    13261 accgccgagg ccgccaaagc cgcttatgag gccgcgggca tcggcccgga ggacatcgac
    13321 gtcgcgcagc tgcaggacac cgacgccggc tccgaagtca ttcacatggc cgagacgatg
    13381 ttgtgcaagg acggcgaaca ggaggcgctg ctcgccgacg gcgccaccca gatcggcggc
    13441 aggctgccga tcaacaccga cggcggcctg ctggccaacg gcgagccggt cggcgcctcc
    13501 ggactgcggc aggtgttcga attggtccac cagctacgcg gtacagcagg cgatcggcag
    13561 gtacccagcg atccgcgggt ggcgctggcg cagctgtacg gggcgccggg taccgctgcg
    13621 gtcgcgatcc tctccagata gcccgacgaa agggagtagt gccaatgaca gacactcagg
    13681 cggaagcccg gcgccgcgcc gaggtcgacc tcgaccacca ttctcccgaa ttccgtgaag
    13741 acccgtacgg cagattccgc gaaatgcgcg aatcaggttg tccagtagca cattccaacc
    13801 attatgaggg attctgggcg ctggccgact acgcatcggt gttcgaagcc gcccgcgacg
    13861 acgacttgtt caactcgttc ccgtccgtcg gcgtccccgc cagcgagctg ccgctgccga
    13921 tcctgcccat cgagtcggac ccgccggaga cgcaggagtt gcgcgaggtc accctcaagc
    13981 ggttctcgcc gggcgcggcg gagcgattcc gcgaatccgc gatcgaaatg accgacgagg
    14041 ccatcgacga gttcatcgag cgcggcgaat gtgatctcgt cggcgcgttg accacgccgc
    14101 ttcctgcccg gttgatcctg cggctgctga agtttgatga gtcgcggcat atggattggg
    14161 tgagctgggt gcacagcacc gtgcacgatc gcgcccacga ccccgagaag gccggcatgg
    14221 ccggtatgga catgttcggc gagatcgtca agcacatgga ggagcgacgg gcggagggcc
    14281 tcggtgacga tctgttcagc gacatccttc gcgggacact gaacggcaag ccactcgacg
    14341 acggccagat caccatgtac accgtgctca tgatgctggg cggcatggac accaccagcg
    14401 ggttcaccgg caacgtgctg tcccagttgt gccgggatcc cggactgcgt caacagttca
    14461 tcgacgatcc aggcctggtc aagaaaggca ccgacgaact gctacggctt tacaccccca
    14521 ccctgggcct ggcccgaacg gtctcgcgag acgccgaatt ccacggccag ccgttgtgca
    14581 agggcgacag ggcgatcctg ctgtgggcgg cggccaaccg cgacccggcg atgttcgacg
    14641 accccgacaa actcgacctg tcccgcccga atgccaagaa acagatggcg tttggggtcg
    14701 ggatgcaccg ctgcctcggg tcccactatg ccaagatgat gttcgacgtc atggtgacgc
    14761 agattttgaa gcggttgccc gacttcgaac tcgcgggcga gccgaaaatg ttcgaggatg
    14821 cgggcgaggt gtacgccgtg cgggagctgc cggtgaagtt caccccgggt ccgcgcaaag
    14881 gctgaccggt ggcctcggtc gccgtcggcg atgtcgaaat cgattacgcg gatacgggtt
    14941 ccgggccggt ggtggtgttc gtccacggcg cgtacgtcac cggtcggctg tggcaggacg
    15001 tggtggaccg actgtccgac aaccatcgct gtatcgcccc gacgctgccg ttcggcgctc
    15061 agaccgaacc ggtcggtgcc cacgtcgatc tcggcgtcgt cgcgtcgggc acgttgatcg
    15121 tcggcctcct cgaagcactc gatctgtccg acgtcacgct ggtggccaac gacaccggtg
    15181 gcgggatcgt gctcgcctcg ctggggaacc cgactctcga ctgggaccgg gtgtcgaagt
    15241 tggttttcac caattgcgat agcttcgaac actttccgcc aagcgccttc gcgccgatcg
    15301 taaggttgtg tgcgctcagc caagcgctgg gcgcgatcgt gctgaagggg ctgaccacgt
    15361 ccgcgggcct gaaggtgttc acgtcggccg tgacccgcca tggcatcgac gctgcgcgcc
    15421 gccccgcgat cttcggcggc ttcctgacct cggccacggt gcgccgcgag gccgcgaggt
    15481 tcaccgccga cctgaagccc agtcacacca tggcggcggt ggaggcgttg aagcgttggg
    15541 acaagccggt gctgatggcg tggggtaccg acgacaagat gtttccgccg gctcacgccc
    15601 accgcctcgc cgagacg
//
LOCUS       DASYLX010000318         6213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1219757,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000318 DASYLX010000000
VERSION     DASYLX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219757"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGP27_07740"
     CDS             complement(<1..196)
                     /locus_tag="VGP27_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851241.1"
                     /translation="MSVAQACAEAGSVTQGARSLFGSQPLPTNGGAQGAAAMRQAQQQ
                     TTSTVSAMGDNSGAARDSHQE"
     gene            complement(235..1428)
                     /locus_tag="VGP27_07745"
     CDS             complement(235..1428)
                     /locus_tag="VGP27_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV7851242.1"
                     /translation="MTRLRINALRALVLTLIVVSGWLVVQAVDPRVTEDDVRGMREAA
                     ARKAGEDFLAQYVERDGRVVRRDEGGDVVSEGQAYGMLIAVAVDDETRFRAIWDWTKS
                     NLRRPDGLLSWRWADGRITDANSAADADLDAARSLLLAGRRFKAPEFTDDGKQLGADI
                     LQAEAVTVGTRTAPPSDLAPPGEWVIGEGRAIAAGNWATAAPYTLNPSYFSPRAEREL
                     MQASADPRWQEISRTQRTLSWQLVGAGQLPPDRATVNEVGDAVSTGTPPGGPMYFGLD
                     AARLPIRFAESCDPSDRAVAAAMRPIIATPGDVPALRNLDGSAASDWQHPVAIVAAAA
                     ADAAGGDSDAAAARLDEASALQWRYPTYYGSAWVALGRIMLASSLLGDCPAGGARFLP
                     APYRR"
     gene            complement(1425..4106)
                     /locus_tag="VGP27_07750"
     CDS             complement(1425..4106)
                     /locus_tag="VGP27_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7851243.1"
                     /translation="MNRLKGFNPSIPTVGILLVLTGILRLINVAGSPARLDDEGTYIA
                     QAFAIIEWGELAHYTYWYDHPPAGWLQLALWMMVSGPGFGENAVVAGRYLMVVVAVIT
                     AGLLWILARRIGLSRWAAAAAVAIYAVSPLAISLSRSVYLDNLAVAWLLGALVLICSP
                     KHRLSAMFGGAICYGVAVLTKETLLLFVPMLAYLVWIKTAPATRRYALAVFAAVFGVV
                     VSTYILMAVLRGELVPGPDHVSLWDGIKFQLWQRADGGAISDPGSLKRHTIDGWLQLD
                     PVLPLLAAPIALFALFVERLRPFAIGLVILVVTIVRPGYLPVPFVITALPLMALLAAG
                     VGEVGLARLRRATRAPSAYARHLRTAAVAAAALVASIVVSLWLPSYHSVLAQDDDAPM
                     REAQQWITENVPKGDRLIVDDAMWADLIRDGRDRRNVIWSYKVDTDEQVQAWSPDGWA
                     DYEWVVSTASMRANMPPDGVLTDAVSHAKPAALFGSSGRRIELLRVDPGSASSKPASP
                     AAPTFGTQVAARLDPRSNPDAVAALQSRRVDQRVVAVLSVLATTEPIVLQNISAIADE
                     EVAGTPRRTFTLTGPAEHLSGLATFFRRQEGPFAVESAEIADGALTVRFPVGGTDITL
                     ADNPPPAPAGDASLRVADMRRTPAADRLEFLRIDGTAAGSLDPGGAPNPSYYRSMPAG
                     TYTLMTIRDGGGPVLRQAFTLTAGMSYTLTLFSGADSTEIAAQLAPDGPPGGPAAGPT
                     LRLLQAAADAGPVKLALAPPGGEPTVLADNASYGLVTGYAPLAPGHYDAVVTAGGREW
                     HRPVELTNPEPTTLVLTDGPDGPALAPTPDVAGAALPLDPPALTMPAAEGQPDKPPAK
                     RVVITDPRQQVFPLGLCGIGFVVGVALVVRTRRRQRR"
     gene            complement(4103..5692)
                     /locus_tag="VGP27_07755"
     CDS             complement(4103..5692)
                     /locus_tag="VGP27_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7851244.1"
                     /translation="MPRLNRGGVAFGAALLMFVAFLIFGGRLMGGDESHSGAPPSVPS
                     TSVEGASTAQAAPAAGGQPGQGARPVGASGGALPQADGVQAVSADPPKAEPPLAQRDD
                     STSWPEFVLYGLLLGVSLVLFVVAATTLWWMLHAWHSPEALAATGFRRHSARGPTTFS
                     LLLPARHEQDVLGDTIDTLTRLDHPAYEVIVIIGHDDPETERVARAAASRRPSLVRVV
                     IDHSVPKNKPKALNTALRQCRGDIVGVFDAEDEVHRGLLRLVEARFEESGADIVQAGV
                     QLMNVRSTWWSLRNCLEYYFWFRSRLHFHADQRFIPLGGNTVFARTDLLRAAGGWDPN
                     CLAEDCEIGVRLSTRGARVAVAYDPRVVTREETPGSIKALVKQRTRWDQGFMQVYRKG
                     EWKKLPTRRQRWLARYTLAMPFLQAATGALVPVAIVSMLVLKVPVPITLLTFLPLAPT
                     LVTLAVESAALGEFGRAFGIRIRLWDHVRLVLGAFPYQLLLAAAAVRSVWRQVRGEGG
                     WEKTAHANVHRNTATPDRRAA"
     gene            5987..>6213
                     /locus_tag="VGP27_07760"
     CDS             5987..>6213
                     /locus_tag="VGP27_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851245.1"
                     /translation="MDYGLVLFTSDRGIAPAAAAKLADDHGFSTFYVPEHTHIPIKRE
                     AAHPTTGDESLPDDRYMRTLDPWVSLGTACAV"
BASE COUNT         1055 a         2166 c         2063 g          929 t
ORIGIN      
        1 actcctggtg actgtcgcgt gccgcgccgg aattgtcgcc catcgcggag acggtgctcg
       61 tggtttgctg ctgtgcctgg cgcatcgccg ctgcgccctg ggcgcccccg ttcgtaggca
      121 gcggctgact cccgaacagg gaacgggcgc cttgggtgac gctgccggcc tccgcgcacg
      181 cctgtgcaac cgacacgcac gaattttcac atgcaactca ggcgcggcct tgcgctatct
      241 tcggtacggc gccggcagga aacgggcgcc acctgcgggg cagtcgccca gcagagacga
      301 ggccagcatg atgcggccca gcgccaccca ggctgacccg tagtaggtcg ggtaccgcca
      361 ctgcagggcc gatgcctcgt cgagccgggc cgccgcggcg tcggaatcac cgccggctgc
      421 gtcggccgct gccgccgcga cgatagcgac gggatgctgc cagtcgcttg ccgccgagcc
      481 atccagattt cgcagagccg gcacatcgcc gggtgtcgcg atgatcgggc gcatggccgc
      541 ggccaccgcg cgatcggacg gatcacacga ctccgcgaac cggatgggaa gccgcgcagc
      601 atccagaccg aagtacatgg gcccgcccgg cggcgtaccc gtcgacaccg cgtcgccgac
      661 ctcgttgacg gtggccctgt cgggcggcag ctgccccgca ccgaccagct gccaactcag
      721 cgtccgctgg gttcggctga tctcctgcca gcgcggatcg gctgaagcct gcatcagctc
      781 gcgttcggct cgaggactga agtacgaggg gttgagggtg tacggcgcag cggttgccca
      841 gttgcccgct gcgatcgccc gaccctcgcc gatcacccac tcgccgggcg gcgccagatc
      901 gctcggcggc gcggttctgg tcccgacggt taccgcttcg gcctgcagga tgtcggcgcc
      961 gagctgcttg ccgtcgtcgg tgaactcggg tgccttgaag cgccggcccg cgagaaggag
     1021 tgagcgagcc gcatcgaggt cggcgtcggc agcgctgttg gcgtcggtga tccgcccgtc
     1081 ggcccatcgc caggacagca gcccgtcagg cctgcgcaga ttcgacttcg tccaatccca
     1141 gatcgcgcgg aagcgcgtct cgtcgtcgac cgctaccgca atgagcattc cgtaggcctg
     1201 gccctcactg acgacgtcgc cgccctcgtc ccgacgcacg acccgaccat cgcgctcgac
     1261 gtactgtgcg aggaagtcct cgcccgcctt acgcgcagcc gcttcccgca tgccgcggac
     1321 gtcgtcctcg gtgacgcgag gatcgacggc ctgaacgacc aaccagccgc tgacgacgat
     1381 cagcgtcagc accagcgcac gcagcgcatt gatccgaagt cgtgtcatcg ccgctgcctc
     1441 cttcgcgtcc tgacgaccag cgccacaccc acgacgaagc cgatgccgca cagcccaagt
     1501 gggaacacct gctggcgcgg atccgtgatc accacccgct tggcaggcgg cttgtccggc
     1561 tgcccctcgg cggcgggcat cgtcagcgca ggcggatcca gcggaagagc ggcgcccgcc
     1621 acgtccgggg tgggcgcaag cgccggtccg tccggaccgt cggtcaacac cagcgtggtc
     1681 ggctcggggt tggtcaactc gacgggtcgg tgccattccc gcccgccggc cgtgacgaca
     1741 gcgtcgtagt ggcctggtgc cagcggcgcg tatcccgtga cgagtccata ggatgcgttg
     1801 tccgccagca ccgtcggttc gccgccggga ggtgcgagcg cgagcttgac cggccccgca
     1861 tcggccgcgg cctgcagcag ccgaagcgtt ggtcccgctg ccggaccgcc aggtggcccg
     1921 tcgggtgcca gctgcgccgc gatctccgtg gaatccgccc cggaaaacag cgtcaaggta
     1981 tacgacatgc cggctgtgag cgtgaaggcc tggcgcagca ctggaccgcc accgtcgcga
     2041 atcgtcatca gcgtgtacgt ccctgccggc atcgaccggt agtaagacgg gttcggcgcg
     2101 ccaccgggat cgagcgaacc ggcggctgtg ccgtcgatgc gcaggaactc cagacggtcg
     2161 gcggccggcg ttcggcgcat gtcggccacg cggagcgacg catcacctgc gggagcgggc
     2221 ggcggattgt cggcgagcgt gatgtctgtt ccacccaccg ggaatcgaac ggttagcgcg
     2281 ccgtctgcga tctcggccga ctcgaccgcg aacggccctt cctgcctgcg gaagaacgtg
     2341 gcgaggcccg acaggtgctc ggccggtccc gtcagcgtga aagtacgtcg cggagtgcct
     2401 gcgacctcct catcggcaat cgccgaaata ttctgcagca caatcggttc tgtggttgcg
     2461 aggacggaca agaccgcgac gacacgctgg tcgacccgcc gcgactgcag tgcggccacc
     2521 gcatcgggat tgctgcgggg atcgagacgc gcggcgacct gcgtaccgaa ggtcggcgcc
     2581 gccggtgaag ccggcttgga cgaagccgag ccaggatcga cacgcagcaa ctcgatacgc
     2641 ctgccgctcg agccgaacaa cgccgccggc ttggcatgcg acaccgcgtc ggtcagcacc
     2701 ccgtcgggag gcatgttggc ccgcatcgat gccgtcgaca ccacccactc gtagtcggcc
     2761 cagccgtcgg gcgaccaggc ctgcacctgc tcgtcggtgt ccaccttgta ggaccagatg
     2821 acgttgcggc ggtcgcgacc gtcgcggatg aggtcagccc acatcgcgtc gtcgacgatc
     2881 agacggtcac ccttgggcac gttctcggtg atccattgct gggcttcgcg catcggggcg
     2941 tcgtcgtctt gcgcgagcac gctgtgatag ctgggcagcc acagcgatac gacaatggag
     3001 gcgacgagcg ccgctgcggc caccgccgcg gtgcgcagat gccgggcgta cgccgacggt
     3061 gcccgggtcg cgcgccgcag gcgggccaac cccacctcgc cgacgccggc ggccagcagc
     3121 gccatcaacg gcaacgccgt gatcacgaag ggaaccggca ggtatcccgg ccgcacgatg
     3181 gtgacgacga ggatcaccag tccgatcgcg aacggtcgga gacgctcgac gaacagcgcg
     3241 aacagggcga tcggcgcggc cagcagcggc agcaccgggt cgagctgcag ccagccgtcg
     3301 atggtgtggc gcttcagcga ccccgggtcg ctaatggcgc caccgtcggc gcgctgccac
     3361 agctggaact tgatgccgtc ccacaggctg acgtgatcgg gcccgggcac gagctcgccg
     3421 cgcaggacgg ccatcaggat gtaggtgctc accaccacgc cgaagacagc ggcgaacacc
     3481 gccagcgcgt agcggcgggt ggcgggcgcc gtcttgatcc acaccagata cgcaagcatc
     3541 ggaacgaaca gcagcagcgt ctctttggtc agaaccgcga cgccgtagca gatcgcgccc
     3601 ccgaacatgg cagacagacg gtgtttcggc gagcagatca gcaccagcgc gccgagcagc
     3661 caggcgacgg ccagattgtc cagatacacc gagcgactca acgagatcgc caacggcgac
     3721 acggcgtaga tcgccaccgc ggcagcggcg gcccaccgcg acagcccgat gcggcgggcc
     3781 agaatccaca gcaggccggc cgtgatcacg gcgacgacca ccatcagata gcgtccggcc
     3841 accaccgcgt tctcgccgaa gcccgggccg gacaccatca tccacaacgc cagttgcagc
     3901 cagccggcgg gcgggtggtc gtaccagtag gtgtagtgcg cgagttcgcc ccactcgatg
     3961 attgcgaaag cctgagcgat gtaggtgccc tcgtcgtcga gccgcgcagg cgacccggcc
     4021 acgttgatca gccgaagaat cccggtgagc accagcagga ttccgacagt agggatcgac
     4081 gggttgaagc ctttcagtcg gttcatgccg ccctccgatc gggggtggcg gtgttgcgat
     4141 ggacgttggc gtgcgcggtt ttctcccagc caccctcgcc gcgcacctgg cgccagaccg
     4201 atcgcacggc tgcggccgcc agcaacagct ggtacgggaa cgcgccgagc accagacgca
     4261 cgtggtccca cagccggatc cggataccga aggccctgcc gaactcgccg agtgcggccg
     4321 actcgacggc gagcgtgacg agtgtcggag ccagcggcag aaacgtcaga agcgttatcg
     4381 gcacgggcac tttcaacacc agcatcgaca cgatcgccac cggcaccaac gcgccagtcg
     4441 ccgcctgcag gaacggcatc gccaaggtgt agcgcgccag ccaacgctgg cggcgggtcg
     4501 gaagcttctt ccactcgccc ttgcggtaga cctgcatgaa accttggtcc cagcgggtgc
     4561 gctgtttgac cagcgccttg atcgaccccg gcgtctcttc gcgcgtcacc acccgcgggt
     4621 cgtaggcgac ggcgacacgc gctcctcggg tggaaagccg cactccgatc tcgcagtcct
     4681 cggcgagaca gttggggtcc cagcctcctg cggcccgcag caggtcggtc cgggcgaaca
     4741 cggtattgcc gccgagcggg atgaaccgct ggtcggcgtg aaagtgcagt cgggaccgga
     4801 accagaagta gtactcgagg cagttgcgca gcgaccacca ggtggagcgc acgttcatca
     4861 actgcacgcc cgcctgcacg atgtcggcgc cggactcctc gaaccgcgcc tcgaccaggc
     4921 ggagcaagcc gcggtgcacc tcgtcttcgg cgtcgaagac cccgacgatg tcgccccggc
     4981 actgccgcag cgctgtgttg agcgccttgg gcttgttctt cgggacgctg tggtcgatga
     5041 ccaccctgac gaggctgggt cgccgagacg ccgcggcgcg cgctacccgc tcggtctcgg
     5101 ggtcgtcgtg accgatgatc acgatcacct cataggctgg atgatcgagc cgggtcagcg
     5161 tgtcgatcgt gtcgccgagc acgtcctgtt cgtgacgcgc aggcaacagc agtgagaacg
     5221 tcgtcggccc acgtgcggag tgacgccgaa acccggtcgc cgccaatgct tctgggctgt
     5281 gccatgcgtg cagcatccac cacagcgtcg ttgcggcgac gacgaacagc accagcgaca
     5341 cgccgagcag cagaccgtac agcacgaact ccggccacga cgtcgagtcg tcacgctgcg
     5401 ccagcggtgg ctcagccttt ggcggatctg cggataccgc ctgcacaccg tcggcctgcg
     5461 gcaacgcgcc gccggaggcg ccgaccgggc gtgcaccctg gccgggctgc ccacccgcgg
     5521 ccggtgcggc ttgggccgtg cttgcaccct cgacgctggt ggacggcacc gaggggggcg
     5581 ccccggaatg actttcgtcg cctcccataa ggcgtccgcc gaagatcagg aacgcaacga
     5641 acatcaacaa ggcggcgccg aaggcgacac cgccacgatt taggcggggc atttgcttgc
     5701 ctcaaagtcg tgttggtcta tcgcgagcgg tgtgcctcag ttctcgagca cggtgaagcg
     5761 gttgagaatg tggtactcgt catgcatatc tttgccttgt acatacatcg ttaccgcgca
     5821 aagcgattcg cccccgtcga tgccgctcat acccggaact cttacaggtc aatcctcaat
     5881 gagacctata tcacttcgtc gcgcagcgaa ccgcattgac gcgtccgcgg tcagcttcga
     5941 taccccgccc ttgcccctca gactggaaca cgttctaatt ctgggtatgg actatgggct
     6001 cgtgttgttc accagcgatc gagggatcgc tcctgcggcc gccgccaaac tggccgacga
     6061 ccacggattc agcaccttct acgtgcccga gcacacgcac atcccgatca agcgcgaagc
     6121 cgcgcacccg acgaccggcg acgagtcgtt gcccgacgac cgctacatgc gcacgttgga
     6181 tccgtgggtc tcgctgggta ccgcgtgcgc ggt
//
LOCUS       DASYLX010000319         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1988722,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000319 DASYLX010000000
VERSION     DASYLX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988722"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="atpD"
                     /locus_tag="VGP27_07765"
     CDS             complement(<1..980)
                     /gene="atpD"
                     /locus_tag="VGP27_07765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV7851246.1"
                     /translation="MTATAEKKTGTDTAGRVVRVTGPVVDVEFPRGSVPELFNALHAE
                     IDYKDLAKTLTLEVAQHLGDNLVRCISLQPTDGLVRGVEVTDTGNSISVPVGDGVKGH
                     VFNALGDCLDDPGYGEDFERWSIHRKPPPFDQLEPRTEMLETGLKVVDLLTPYVRGGK
                     IALFGGAGVGKTVLIQEMINRIARNFGGTSVFAGVGERTREGNDLWVELADADVLKDT
                     ALVFGQMDEPPGTRMRVALSALTMAEFFRDEQEQDVLLFIDNIFRFTQAGSEVSTLLG
                     RMPSAVGYQPTLADEMGELQERITSTRGHSITSMQAVYVPADDYTDPAPAT"
     gene            complement(1003..1929)
                     /locus_tag="VGP27_07770"
     CDS             complement(1003..1929)
                     /locus_tag="VGP27_07770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV7851247.1"
                     /translation="MAATLRELRGRIRSAGSIKKITKAQELIATSRIAKAQARVQAAR
                     PYDREITHMLSNLANASALDHPLLVERENPRRAGVLVVSSDRGLCGAYNANVFRRSEE
                     LFSLLRSEGKEPVLYTVGRKALGYYSFRNWDVVDSWTGFSERPDYENAQEIASTLVDA
                     FTAGMDDEGDDPGEDDVLGVDELHIVFNEFKSMMSQTAVARRIAPMVVEYSEEETGPQ
                     TLFSFEPDAETLFGALLPRYVATRIFAALLEAAASESASRRRAMKSATDNADDLIKDL
                     TLMANRERQSQITQEISEIVGGANALADAANK"
     gene            complement(1942..>2177)
                     /locus_tag="VGP27_07775"
     CDS             complement(1942..>2177)
                     /locus_tag="VGP27_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV7851248.1"
                     /translation="LEHVKGSHEDILKEIRESKKLSEELEEKLEKVVNDFKKGFDTTD
                     GSSVVPDEHADAMDEEDVEKESVKVNKPKPKKK"
BASE COUNT          349 a          700 c          688 g          440 t
ORIGIN      
        1 gtggcgggcg ccgggtcagt gtagtcgtcg gcgggcacgt acacggcctg catcgaggtg
       61 atcgagtggc cgcgggtcga cgtgatgcgc tcttggagct cacccatctc gtcggccagt
      121 gtcggctggt atcccaccgc ggaaggcatg cggcccagca gtgtggacac ctcggagccg
      181 gcctgtgtga accggaagat gttgtcgatg aacagcagca cgtcctgctc ttgttcatcg
      241 cggaagaact cggccatggt gagcgcagac aacgcgaccc gcatacgggt gccgggcggc
      301 tcatccatct gaccgaacac cagcgccgtg tccttgagca cgtcggcgtc tgcaagctcg
      361 acccacaggt cgttgccctc gcgggtgcgc tccccgaccc cggcgaacac cgacgtgccg
      421 ccgaagttgc gggcgatgcg gttgatcatc tcctggatca atacggtctt gcccacgccg
      481 gcgccgccga agagggcgat cttgccgccg cggacgtagg gcgtgagcag gtcgacgacc
      541 ttgaggccgg tctccagcat ctccgtgcgg ggttccagct gatcgaaagg cggcggcttg
      601 cggtggatcg accagcgctc gaagtcctcg ccgtagccgg gatcatcgag gcagtcgccg
      661 agcgcgttga acacgtggcc cttgactccg tcgccgacgg gtaccgagat cgagttgccg
      721 gtgtcggtca cctcgacgcc ccgaaccaga ccgtccgtgg gctgcaggga gatgcagcgc
      781 accaagttgt cgccgaggtg ctgggcaacc tccagcgtca gcgtcttcgc cagatccttg
      841 tagtcgatct ctgcgtgcaa cgcgttgaac aactcgggca cagaaccacg cgggaactcg
      901 acgtcgacga cggggccggt gacgcgcacg acgcggcccg cggtgtcggt gccggtcttc
      961 ttttcagcag tagcagtcat gtctcttttc gcttcctacg ggctacttgt tggcggcgtc
     1021 cgcgagcgcg ttagcgccac cgacgatttc gctgatttcc tgagtgatct gggattgccg
     1081 ttcgcggttg gccatcaggg tcaggtcctt gatgaggtcg tcggcgttgt ctgtcgccga
     1141 cttcatcgcc cgccggcggg atgccgactc cgatgccgcc gcctcgagca gggcggcgaa
     1201 gatgcgggtg gcgacatatc gtggcagtag cgcgccgaac agtgtttccg catccggctc
     1261 gaacgagaac agcgtctgcg gtccggtctc ctcttcgctg tactccacca ccatcggcgc
     1321 gatccggcgc gcgaccgcgg tctgcgacat catcgatttg aactcgttga acacgatgtg
     1381 caactcgtcg acgccgagaa cgtcgtcctc gccgggatcg tcgccctcgt cgtccatgcc
     1441 tgcggtgaag gcgtcgacca gtgtcgacgc gatctcctgc gcgttctcgt aatcaggccg
     1501 ctcggagaac ccggtccagg agtcgaccac atcccaattg cggaagctgt agtacccgag
     1561 agcttttcga cccaccgtgt acagcacggg ttccttgccc tccgaacgaa gcaaggagaa
     1621 caactcctcc gaccgccgaa agacgttggc gttgtacgcg ccgcagagcc cacggtccga
     1681 cgacaccacc agcacaccgg cgcgccgtgg gttctcgcgt tcgaccaaca gcgggtggtc
     1741 caacgcgctc gcgttggcca agttcgacag catgtgggtg atttcgcggt cgtaaggccg
     1801 agccgcctgt actcgggcct gtgccttggc gatacgcgag gtcgcgatca gctcctgggc
     1861 cttggtgatc ttcttgatcg acccggcgga gcggatacgt ccacgcagtt cgcgcagtgt
     1921 ggcagccatt agcgctccgg cctatttctt cttcggcttg ggcttgttga ccttcaccga
     1981 ttccttctcg acgtcttctt cgtccattgc atcggcgtgc tcatcgggga cgactgagct
     2041 gccgtcggtg gtgtcgaagc ccttcttgaa gtcgttcacg actttctcga gcttttcctc
     2101 gagctcctcg gagagcttct tgctctcccg aatctctttg aggatgtcct catgcgagcc
     2161 cttcacgtgc tcgagaa
//
LOCUS       DASYLX010000320         5869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_233152,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000320 DASYLX010000000
VERSION     DASYLX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233152"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGP27_07780"
     CDS             <1..217
                     /locus_tag="VGP27_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7851249.1"
                     /translation="IAGVSNTVAALVFAFTAPVQWLAAIALGAGCLVGSRLGPIVVRH
                     APAGPLRALIAAAGVALAVKLGMDAYG"
     gene            complement(214..1281)
                     /locus_tag="VGP27_07785"
     CDS             complement(214..1281)
                     /locus_tag="VGP27_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV7851250.1"
                     /translation="MPDEAPQLPTLSDHDQAAVQRWIRQQGLGSDVTDVEPLTGGTQN
                     IVVRVHVDGRPMVLRRPPLHPRPTSDKTMLREIAALRTLAGTDVPHPGFIAGCDDLDV
                     LGVVFYLMEEIDGFNPGSEMSEAYVRDAGMRHDVGLSYAASLARLGNAAWEDKPLAEL
                     KRPGSFLGRQVPQFLRLLESYRHGRYDPESLAVTDLAEWLQSDRPPDAEPGILHGDPH
                     LSNVLLRRDRPELAAFVDWEMCTVGDPLLDLGWMLITWPLNTSTITAGGALAALGGLA
                     TRKELLDAYWTAGGRQTSRLDWYMAMACFKLGIVIEGTWSRYLIGQASRDAGEALHAN
                     AQNLIDMGTRVAKGDSPFDLD"
     gene            complement(1294..2118)
                     /locus_tag="VGP27_07790"
     CDS             complement(1294..2118)
                     /locus_tag="VGP27_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7851251.1"
                     /translation="MTSPTPTVDQAVIAKHRAMWATGDYPKLAIELVAPLGPILVDAT
                     GCGPGDRVLDVAAGTGNAAIPAAKTGATVIASDLCPELLQAGAALASDQGVDLEWREA
                     DAHALPFGDSDFDVVMSCIGVMFAPFHEQSAGELIRVCKPGGRLGLLSWTPEGHIGQL
                     FATMKPYMPPPPPGAQPPPLWGREDHVRELLGDRVTDVVTERRTLTVDQFATGAEFRD
                     YFKNVYGPTIAAYRNIEGDTDRTATLDADIARVGDGFLENSDTMEWEYLLLTARKA"
     gene            2332..3006
                     /locus_tag="VGP27_07795"
     CDS             2332..3006
                     /locus_tag="VGP27_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7851252.1"
                     /translation="MSGYGQFCPVAKAMELLDERWTVLVVRELLAGSTHFNDLRRGVP
                     KMSPALLSKRLQTLIRAGVVERTEVDGRTTYTLTSSGKELVDVVEALGAWSVRWIGEL
                     GDEDLDPHLLMWDIRRTVPIEAWPRSRTMLAFHLDDVAPKASRWWLVVAEGQADVCDF
                     DPGYDVAATIQTSLRMLTRIWRGDVSWRHALLDGSVIISGEADVRRAVPSWIGQSSLA
                     AVPRPA"
     gene            complement(3085..4818)
                     /gene="poxB"
                     /locus_tag="VGP27_07800"
     CDS             complement(3085..4818)
                     /gene="poxB"
                     /locus_tag="VGP27_07800"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEV7851253.1"
                     /translation="MATIADHVISALALSGVHRVYGLPGDSLNGFTDAIRRAPEMSWE
                     HVRHEETAAFAAAADAALTGALAVCAGSCGPGNLHLINGLFDAQRSRVPVLAIAAHIP
                     RSEIGSQYFQETHPQELFRECSVYCELVSTAEMTPRILEMAMRAAVEERGVAVVVIPG
                     EVFLQRAESAWRARPVAAARSVVRPDDESVQRAATILNDARAVTILAGAGVEGSHDAV
                     IAAAATLNAPIVHALRGKEFIEHDNPFDVGMTGLLGFASGYKAIKDADALLMLGTDFP
                     YQQFYPDNAKVVQVDIRGRSLGRRTPIELGLVGSVRDTLAALQPSLRQKQNRDHLDRS
                     LNHYRKTRKRLDELAVNDRDRTPIRPEYVAGLVNRLGADDAVFTVDVGSPVVWAARYV
                     GMNGMRRLIGSFNHGTMACAVPLAIGAQTAHRDRQVVALAGDGGLTMLFGELITLTQN
                     RLPVKVIVFNNSSLNFVELEMKAAGIVNFGTDLLNPNFADVATAMGIFGRRVEHPSEL
                     ERAITDAFAYDGPAVVDVVTARQELSIPPAITVEQAKGFSLYAIRTILAGRGEELLDL
                     VTTNVARRILD"
     gene            complement(4844..5611)
                     /locus_tag="VGP27_07805"
     CDS             complement(4844..5611)
                     /locus_tag="VGP27_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851254.1"
                     /translation="MEIAGKKAIIVGGASGFGKATAEALAKRGAAVAILDQPQSKGKE
                     VAEAVGGTFHEVDVTDFEGTEKTLQQAIDAIGGLHIAVTTAGGGKGERTISKDGPHSL
                     ESFRKGIDLNLIGTFNISRLAAWQMSKNDPVDDEAEERGVIINTASIAAFEGQIGQVA
                     YTASKAAIAGMCLTMARDLGSLGIRVLAIAPSLFATGLTEGIPDDFAKVLTKDAAFPK
                     RLGRPDEYAKLAVAIVENAMLNGQCLRLDGGQRFAPK"
     gene            5711..>5869
                     /locus_tag="VGP27_07810"
     CDS             5711..>5869
                     /locus_tag="VGP27_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851255.1"
                     /translation="MASSGIRATAGVAAAAVALGVTQLLAAFVGPRADSRTAIGSTVI
                     DLTPGPVKE"
BASE COUNT          929 a         1927 c         1957 g         1056 t
ORIGIN      
        1 catcgccggt gtctccaaca ccgttgcggc gcttgtcttc gcgttcaccg ccccggtgca
       61 gtggctcgcc gccatcgcgc tgggcgccgg ttgcctggtc ggatcgaggc ttggtcccat
      121 cgtggtgcgc catgctcctg cggggccact gcgggcgttg atcgccgcgg cgggcgtcgc
      181 actggccgtc aaactgggaa tggacgcata cggctagtcc aggtcaaagg gactgtcgcc
      241 cttggcgacc cgtgtcccca tatcgatcag gttctgcgcg ttggcatgta gggcctcgcc
      301 tgcgtctcgg ctcgcctgtc cgatcagata gcgcgaccag gtgccttcga tgacaatgcc
      361 gagcttgaag catgccattg ccatgtacca atccagccgc gatgtctggc ggccgcccgc
      421 ggtccagtac gcgtcgagca gttctttccg ggttgccaac ccgccgagcg cagccagcgc
      481 acccccggcg gtgatcgtgc tggtattcag cggccaggtg atcaacatcc aaccaagatc
      541 caatagcggg tcgccgaccg tgcacatctc ccagtcgacg aacgccgcga gctccggcct
      601 gtcccggcga agcagcacat tgctcagatg cggatcgcca tgcaggattc ccggctcagc
      661 gtccggcggc cgatcggact gcagccactc cgcgagatcc gtgacggcca gcgattcggg
      721 gtcatagcgg ccatgccggt agctctcgag cagtcggagg aactgcggaa cctggcgccc
      781 cagaaaggat cccggccgtt tgagctccgc gagcggcttg tcctcccacg cggcgtttcc
      841 cagccgcgcg aggctcgcgg cgtaggacag cccgacgtca tgccgcatcc ccgcgtcgcg
      901 gacatacgcc tcggacatct cgctgcccgg attgaagccg tcgatctctt ccatgaggta
      961 gaaaacgact ccgagcacgt ccaggtcgtc gcagccggcg atgaaccccg ggtgtggcac
     1021 atccgtgccc gccagcgtgc gcagcgccgc gatctcccgc agcatcgtct tgtcgcttgt
     1081 cggccgcggg tgtagcggcg gccgtcgaag caccatcgga cgcccgtcga cgtgcacccg
     1141 cacgacgatg ttctgcgttc caccggtcag cggttcgaca tctgtgacat ccgagcccag
     1201 tccctgctgc cggatccagc gctgcaccgc agcctggtcg tgatcgctca gtgtcggcag
     1261 ttggggcgct tcgtcgggca tgacgacatc gtgtcaggcc ttgcgcgcgg tcaacagcag
     1321 gtactcccat tccatggtgt ccgagttctc gaggaagccg tcgccgaccc gtgcgatgtc
     1381 ggcgtccagc gtcgcggtcc ggtcggtgtc accctcgatg ttgcggtaag ccgcgatcgt
     1441 cggcccgtag acgttcttga agtagtccct gaattcagcg ccggtggcga actggtcgac
     1501 ggtcagggtg cgacgctcgg tgacaacatc ggtgactcgg tcgccgagca gctcacggac
     1561 gtggtcctcg cggccccaca acggcggcgg ctgcgcaccc ggcggaggtg gcggcatgta
     1621 cggcttcatg gtggcgaaga gctggccgat gtggccctcc ggcgtccagc tgagcaggcc
     1681 aagcctgccg cccggtttgc acacccggat cagctcgccg gcggactgct cgtggaacgg
     1741 cgcgaacatc acaccgatac acgacatgac gacgtcgaaa tcgctgtcgc cgaaaggaag
     1801 cgcgtgggcg tcggcttccc gccactccag gtcgacgccc tggtccgagg caagggcggc
     1861 gccggcctgc agcagctcgg ggcacaggtc gctggcgatg acggtggcgc cggtcttggc
     1921 ggcgggaatg gccgcgtttc cggttccggc cgccacgtcc agcacacggt cgccggggcc
     1981 gcatccggtg gcgtcgacca aaatggggcc cagcggggcc accaactcga tggcgagttt
     2041 ggggtagtcg cccgtggccc acatggcgcg atgcttcgcg atcaccgcct gatcgacggt
     2101 cggggttggt gaagtcattt gcgttcctcc gttcgatgcc agctgtgatg ttgctgtgag
     2161 gatcgtgcgc cttccgccgc gcaatggcta gttccgaatc tgtacctgcc cggcggccgg
     2221 atctgagcta gatctgtact gacgatggtt caagatctgt actggtcaat tgagcgatcc
     2281 tgcgatgtac ttgaatctgg gtttgctata cgcgactggg aaggttgtga catgtcgggt
     2341 tacggtcagt tttgtcccgt ggcaaaggcc atggaactgc tggacgaacg ctggacggtc
     2401 cttgtggtgc gcgaattgct ggcaggcagc acccacttca atgaccttcg ccgtggcgtg
     2461 ccgaagatgt cgccggcgtt gctatccaag cggctacaaa cgctgatccg cgccggggtg
     2521 gtagaacgca ccgaggtcga cggccgcacg acatacacgc ttacgtcgag tggaaaggaa
     2581 ctcgttgacg tcgtcgaagc gctcggcgcg tggagcgtcc gatggatcgg cgaactcgga
     2641 gacgaagatc ttgatcccca cctgctgatg tgggacatcc gccgaaccgt tccaatcgag
     2701 gcgtggccgc ggtcgcgcac catgctggcg ttccaccttg atgatgtggc gcccaaggcc
     2761 tcacggtggt ggctggtggt ggcggaggga caggctgacg tctgcgattt cgaccccggc
     2821 tacgacgtgg cggcaacgat ccagacaagc ctgcgcatgc tgacgcgcat ctggcgcggc
     2881 gacgtgagct ggcgccacgc actgctcgac ggcagcgtca tcatttcagg tgaggccgat
     2941 gtccgtcgcg ctgtcccgtc ctggattggc cagagttcac tggcggcggt accgcgcccc
     3001 gcatgagtcc tacccctggt agccgagaca agcttcgcgc caccatgacg ccttcgccat
     3061 ggaagacagc gtcgtaggtt gctctcagtc caggatccgg cgcgcgacgt tggtggtgac
     3121 gaggtcgagc aactcctcgc cgcgcccggc caggatcgtc cggatcgcgt acagcgaaaa
     3181 gcctttcgcc tgttccacgg tgatcgcggg cgggatcgag agctcctggc gggcggtcac
     3241 cacgtcgacg acggcgggcc cgtcgtaggc gaacgcgtcg gtgatggccc gctccagctc
     3301 ggagggatgc tcgacccgac ggccgaagat gcccatcgcg gttgcgacgt cggcgaagtt
     3361 cggattgagc agatcggtgc cgaagttgac gatccctgcc gccttcatct ccagctcgac
     3421 gaagttgagc gacgagttgt tgaacacaat caccttgacg ggcaaccgat tctgggtcag
     3481 cgtgatcagc tcgccgaaga gcatggtcag gcccccgtca ccggccagcg cgaccacctg
     3541 ccggtcgcgg tgggcggtct gggcgccgat cgcgagcggc accgcgcagg ccatggtgcc
     3601 gtgattgaag gagccgatca gccgacgcat gccgttcatg cccacatagc gggccgccca
     3661 caccacagga gatccgacgt cgacggtgaa caccgcgtcg tcggcgccca gccgattcac
     3721 aagcccggca acgtattcgg gacggatcgg cgtgcggtca cggtcgttga ccgccaactc
     3781 gtcgagcctc ttgcgggtct tgcggtagtg gttgagagac cggtcgagat ggtcgcggtt
     3841 ctgcttctgc cgaagcgacg gctgcagggc ggccagtgtg tcgcgcacgc tgccgaccag
     3901 acccagctcg atcggcgtgc gcctaccgag gctccggccg cggatgtcga cctggacgac
     3961 cttcgcattg tcgggataga actgctgata agggaagtcg gtgccgagca tcagcagggc
     4021 atcggcatcc ttgatggcct tgtagccgga ggcgaagccg agcaggcccg tcattccgac
     4081 atcgaacggg ttgtcgtgct cgatgaactc cttgccgcgc agcgcatgca cgatgggtgc
     4141 gttcaaagtc gccgccgcgg cgatcaccgc gtcgtggctt ccttcgacgc cggcaccggc
     4201 gaggatggtg acagcacggg cgtcgttgag gatcgtcgcc gcccgctgca ctgactcgtc
     4261 gtcgggtcgc accaccgatc gggccgccgc caccggcctg gccctccacg cagactcagc
     4321 gcgctgcagg aacacctcgc ccggaatcac cacaacggcg acgccgcgct cttcgacggc
     4381 cgcacgcatc gccatctcga ggatccgcgg tgtcatctcg gcggtgctga cgagttcgca
     4441 gtagacgctg cattcccgga acagctcttg gggatgggtc tcctggaaat actgggagcc
     4501 gatctccgag cgcgggatgt gcgcggcgat cgccagcacc ggtacgcggc tgcgctgtgc
     4561 gtcgaacagg ccgttgatca ggtgcaggtt gccgggccca cagctgcctg cacagaccgc
     4621 gagcgctccg gtcagcgcag catcagcggc cgcggcgaag gcggcggtct cctcgtggcg
     4681 cacgtgctcc cacgacattt caggagcccg acggatcgcg tcggtgaagc cgttgaggct
     4741 gtccccgggc agcccgtaga cacgatggac accgctgagc gcgagcgcgg agatgacgtg
     4801 atcggcgatg gtcgccaccc ggtcacccta tgcgaaacag ctgttacttg ggcgcgaagc
     4861 gctggccgcc gtccagtcgc aggcactgcc cgttgagcat cgcgttctcg acgatggcga
     4921 ccgcgagctt ggcgtactcg tcgggtcggc ccagtcgctt ggggaatgcg gcgtccttgg
     4981 tcagcacctt ggcgaagtcg tcggggatgc cttcggtcag accggtggcg aacaggctcg
     5041 gcgcgatcgc caggacccgg atgccgaggc tgcccaggtc gcgcgccatc gtcaggcaca
     5101 tgcctgcgat cgcggctttc gacgccgtgt aggcgacctg accgatctgg ccttcgaacg
     5161 ccgcgatcga cgcggtgttg atgatgacgc cgcgttcctc ggcctcgtcg tccaccggat
     5221 cgttcttgct catctgccat gcggccagcc ggctgatgtt gaacgtgccg atgaggttga
     5281 ggtcgatgcc cttgcggaag gactccaggc tgtgcggacc gtccttgctg atggtgcgct
     5341 cccctttgcc gccgcccgcg gtggtcaccg cgatgtgcag gccgccgatg gcgtcgatgg
     5401 cctgctgcag ggtcttctcg gtgccctcga agtccgtgac gtccacttcg tggaaggttc
     5461 cgccgacggc ctcggcgact tccttaccct tcgactgcgg ctgatccagg atcgccaccg
     5521 cggcgccacg cttggccaac gcctcggcgg tcgccttgcc gaacccggac gccccgccga
     5581 cgatgatggc cttcttaccc gcgatctcca tctggctccc tttccaaaaa gtgctcgatt
     5641 cgtaaagcaa cctagcggac gtcacttttc ggtaaggctt tcgggtgggc gggtcggtag
     5701 cttggcgacc atggcgtcta gcggcatccg agcgacggca ggcgtcgcgg cggccgcggt
     5761 cgccctcggc gtcacccagt tgctggccgc gttcgtcggc ccgagggccg attcccgcac
     5821 cgcgatcggc tcgacggtca tcgacctcac tcccggaccg gtcaaggaa
//
LOCUS       DASYLX010000321         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1219818,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000321 DASYLX010000000
VERSION     DASYLX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219818"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VGP27_07815"
     CDS             <1..1189
                     /locus_tag="VGP27_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851256.1"
                     /translation="TADEAADLLRNPYPIFERRRRETSGVFCGSVMDWTKASEDFRPE
                     NLYAAVSFDAVNRVFRDGKVFNSHIYDSTVGLFMGPTILAMEGKKHWEHRNLVSAAFK
                     SRSLLRWEPEIVRPVVNALIDEFIDAGEADLVRQFTLEFPTRVISQLLGLPEEDLPFF
                     RKQAVALISYHVKYERAFEASAALKDYFSEQIDKRRSKPTDDIIGDLVSAEIDGEKLD
                     DEAIYSFLRLLLPAGLETTYRSSGNLLFLLLSHPDQFRALQGDRALIGPAIEEGLRFE
                     TPLTTVQRYTAVETELEGLTLPAGAVIDVCIGSANRDEHRWERSEEFDITRKPLPHIS
                     FAAGEHTCLGLHLARMETRVAIECLLDRLTDIQFVTEGNPHIYGQPFRSPTVLPVTFA
                     AAG"
     gene            1254..2468
                     /locus_tag="VGP27_07820"
     CDS             1254..2468
                     /locus_tag="VGP27_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7851257.1"
                     /translation="MEGVRVLEVAQFTFVPAAGAILADWGADVIKVEHPVRGDTQRGF
                     INMGGFELNPQRHPLIEHPNRGKRSVGIDLSTEGGQEVLYEIAKASDVFLTNYMPAQR
                     QKNKFDVEHIRGVNPNIVYARGSAYGDKGPERDTGGFDGTAFWTRSGVGHALTPEELG
                     GALSQGIPAFGDSIGGMNIAGGISAALFHRERTGEAVELDVSLLSTAWWAAGASVTQG
                     METGETMRSLMPDSIGPTVNPFLGNYLTSDGGTINLCIVSPTGYIRDAFEHLGLPELA
                     DDPRFSEVMPLIVNSAEAAQLITEAIRSKPFEYWREHLKTMKGQWAPFQSLLELATDE
                     QAVANDMIVEVEAADGGAPFKVVRGPIQFNHEPLETTRAPQASEHTEIVLAELGMDWE
                     RITELKDSGAIA"
     gene            2679..3824
                     /locus_tag="VGP27_07825"
     CDS             2679..3824
                     /locus_tag="VGP27_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7851258.1"
                     /translation="MDDVAIIGVGLHPFGRFEGKSAMEMAVDAILSAVADAGVDWKDV
                     QFATGGSWTVANPDAIVGMVGLTGIPFTNVFNACATAASAARACADGIRLGDYDIGIA
                     VGLDKHPRGAFTEDPALVGMPSWYAENGQYLTTQFFGMKANRYLHDHGISQATLAKVA
                     AKNFRNGALNPNAFRRKPIAEDDILNSTMLNYPLTQYMFCAPDEGAAAVVMCRADIAH
                     RYTSKPVYVRAVEVRTRKYGAYEVNTTFAPVEEDVAPTVYAAKAAFEKAGVAPDDVDV
                     IQLQDTDAGAEIIHMAECGFCGHGDQEKLLAEGATEIGGSMPVNTDGGLIANGEPIGA
                     SGLRQIHELVRQLRGEAGDRQVAGAPRVGFAQLYGAPGTAAATIVSL"
     gene            complement(3867..>4396)
                     /locus_tag="VGP27_07830"
     CDS             complement(3867..>4396)
                     /locus_tag="VGP27_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEV7851259.1"
                     /translation="YARDRGLPAVAMCVSNPYGPLDWQPHQGLMVKMAALGKMPVYVK
                     DVSTEVVGIEDVAEAFLLAAERGRVGERYIISESFMPMRQMFETAASAVGAKPPRFGV
                     PLAMLYAVGWIMGVASRVLRRDLSMNLTGVRLLHIMSAADHSKATRELGWVPRPTAES
                     IRRAAQFYVEQARSA"
BASE COUNT          809 a         1480 c         1394 g          713 t
ORIGIN      
        1 cacggccgac gaagcggccg accttcttcg caacccctat cccatcttcg agcgcaggcg
       61 acgcgaaacc agcggtgtgt tctgcggcag cgtcatggac tggacgaagg cttccgaaga
      121 cttccggccc gagaacctct atgccgcggt gtccttcgac gcggtgaacc gggtgttccg
      181 cgacggcaag gtgttcaact cccacatcta cgactccacg gtcggtttgt tcatggggcc
      241 gaccatcctg gcgatggagg gcaagaagca ttgggagcac cgcaatctcg tctctgcggc
      301 gttcaagtcg cgctcgctgc tgcggtggga acccgaaatc gtccgcccgg tggtcaacgc
      361 gctgatcgat gagttcatcg acgcgggcga ggcggatctc gtgcgccagt tcacgctcga
      421 gttccccacc agggtgatct cgcagctgct gggactacct gaggaagacc tgccgttctt
      481 ccgcaaacaa gcggtcgccc tgatcagcta tcacgtcaag tacgagcgcg ccttcgaggc
      541 ctcggccgca ctgaaggact acttcagcga gcagatcgac aagcgccgct cgaagcccac
      601 cgacgacatc atcggcgacc tggtgtccgc ggagatcgac ggcgaaaagc tcgacgacga
      661 ggcgatctac tccttcctgc gcctcctgct gcccgcgggt ctggagacca cctaccggtc
      721 gtcgggcaac ctgctgttcc tgttgctcag ccatcccgac cagttccgtg cgctgcaggg
      781 cgaccgcgcg ctcatcggtc ccgccatcga ggaggggctg cgcttcgaaa ctccgttgac
      841 gacggttcag cgctacacgg ccgtggagac cgagctcgag ggtttgacgc tgcctgcggg
      901 cgcggtgatc gacgtctgca tcggttccgc gaatcgggac gaacaccggt gggagcgctc
      961 ggaggagttc gacatcaccc gtaaaccact gccgcacatt tcttttgctg ccggtgagca
     1021 cacctgcctc ggcctgcacc tggcccggat ggagacccgc gtcgcgatcg agtgcctact
     1081 cgaccggttg actgacatcc agttcgtcac cgagggtaat ccgcacatct acggtcagcc
     1141 gttccgctct ccgacggtgc ttccggtgac gttcgcggcg gcgggctgat tcgcgtggac
     1201 tctatgctcg actcatccga cgagccaacg ggagggccga cgtgatcaag gtgatggagg
     1261 gcgtacgggt cctcgaggtc gcgcagttca ccttcgtccc cgcggccgga gccatcctcg
     1321 ccgactgggg cgccgacgtc atcaaggtgg aacacccggt acggggcgat acccagcgcg
     1381 ggttcatcaa catgggcggc ttcgagctga acccgcagcg gcatccgctg atcgagcacc
     1441 cgaaccgcgg aaagcgcagc gtcggcatcg acctctccac cgagggcggt caggaggtgc
     1501 tgtacgagat cgccaaggct tccgacgtct tcctcaccaa ctacatgccc gcacagcgcc
     1561 agaagaacaa gttcgacgtc gagcacatcc gcggggtgaa cccgaacatc gtctacgccc
     1621 gcggaagcgc ttacggcgac aaaggaccgg agcgcgatac gggcggcttc gacggcactg
     1681 ccttctggac gcgcagcggc gtcggacacg cgttgacacc cgaggagctg ggaggtgcgc
     1741 tttcccaagg tattccggcg ttcggtgact ccatcggcgg catgaacatc gccggcggaa
     1801 tctctgcggc cctgttccac cgcgagcgca cgggcgaggc ggtggaactc gacgtgtcgc
     1861 tgctgagcac ggcatggtgg gcggcaggcg ccagcgtgac ccagggtatg gagaccggtg
     1921 agacgatgcg ctcgctcatg ccggattcca tcggccccac cgtcaacccg ttcctcggaa
     1981 actatctgac gtccgacggc ggcacgatca acctgtgcat cgtcagcccg accggctata
     2041 tccgggacgc gttcgaacat ctcggattgc cggaactcgc cgacgacccg cgcttctccg
     2101 aagtgatgcc gctcatcgta aactccgccg aggcagcgca actcatcacc gaggcgatcc
     2161 gaagcaagcc cttcgagtac tggcgggagc atttgaagac catgaaagga cagtgggcgc
     2221 ctttccagag cctgctcgag ctggccaccg acgagcaggc ggtcgccaac gacatgatcg
     2281 tcgaggtcga agcggccgac ggcggagcac ccttcaaggt cgtccgcggc ccgatccagt
     2341 tcaaccacga accactcgaa accacccgtg cgccacaggc atccgagcac accgaaatcg
     2401 tgctggcgga gctcggcatg gattgggagc gcatcaccga gctcaaggac tccggcgcca
     2461 tcgcctaacc ccgtctccat ccccatctcc gcgagcgtgc gtgtctgctc cgcgacacgc
     2521 cgcgcggcgc cgacatctgc gcaccttcgc cgcgtcgcga gcgtgcgcgc ggtgacggcg
     2581 ctgagattgg cgttcgacgc gcgccggccg ccggtcagcc acaccggcgc acaaaacgga
     2641 gatactgtaa gagttacagt tgcgatacac tcggccccat ggatgacgtc gccatcatcg
     2701 gtgttgggct gcacccgttc ggcaggttcg aaggcaagtc cgcgatggag atggctgtcg
     2761 acgcaatatt gtctgcggtc gcagacgcag gagtcgactg gaaggacgtt cagttcgcca
     2821 ccggcggcag ctggacggtt gccaacccgg acgccatcgt cggcatggtc gggctcacgg
     2881 ggatcccgtt caccaacgtc ttcaacgcct gcgccaccgc cgccagcgcc gcgagggcat
     2941 gcgcggacgg cattcgactc ggcgactacg acattgggat agcggtcggg ctggacaagc
     3001 atccgcgcgg tgccttcacc gaagaccccg cactggtcgg catgcccagc tggtatgccg
     3061 agaacggcca atatctcacc acccaattct tcggcatgaa ggccaaccgc tatctgcacg
     3121 accacgggat ctcacaggca acgctggcca aggtggccgc caagaacttc cgtaacggcg
     3181 cgctgaaccc gaatgcgttt cggcgcaagc cgatcgccga ggacgacatc ctcaattcga
     3241 caatgctcaa ttaccccctg acccagtaca tgttctgtgc acctgacgag ggagccgccg
     3301 cggtggtgat gtgccgcgcc gacatcgcgc accgctacac ctcgaagccg gtgtacgtgc
     3361 gcgccgtcga ggtgcgcacc cgcaagtacg gcgcctatga ggtgaacacg acattcgcgc
     3421 cggtcgagga ggacgtggcg ccgaccgtgt acgccgcgaa ggccgccttc gaaaaggccg
     3481 gtgtcgcacc ggatgacgtc gacgtcatcc agctacagga caccgatgcc ggcgccgaga
     3541 tcattcacat ggccgagtgc gggttctgcg ggcacggcga ccaggagaag cttctggccg
     3601 aaggcgcaac cgagatcggt ggctcgatgc ccgtcaacac cgacggcggc ctgatcgcca
     3661 acggcgagcc gatcggtgca tccggcctgc gccagatcca cgaactggtg aggcagttgc
     3721 gcggcgaggc gggcgaccgc caggtggccg gtgcgccgag ggtcggcttc gcccagctct
     3781 acggtgctcc cggcaccgcg gcggcgacga tcgtgtcgct gtagctgacg ctgcggacgc
     3841 gcaagttcag cccgatccga ccggcgtcac gccgatcgcg cctgctcgac atagaactgc
     3901 gccgcccgcc gtatcgactc ggcggtcggg cggggtaccc agcccagttc gcgcgtcgcc
     3961 ttgctgtggt cggcggccga catgatgtgc aggagccgta cgccggtcag gttcatcgaa
     4021 aggtctcgac ggagcacccg gctcgcaaca cccatgatcc agcccacggc gtagagcatc
     4081 gccagcggca caccgaaccg cggcggtttg gcgccgaccg ccgacgccgc cgtctcgaac
     4141 atctgccgca tgggcatgaa gctttcggag atgatgtagc gctcccctac ccgaccgcgt
     4201 tcagcggcga gcaggaacgc ctcggccacg tcttcgatgc cgacgacctc ggtggacaca
     4261 tccttgacat agaccggcat ctttccgagt gcggccatct tcaccatcag gccctgatgc
     4321 ggctgccagt caagaggccc atacgggttg gagacgcaca tggcgacggc cggcaggccc
     4381 cggtcgcggg cgtact
//
LOCUS       DASYLX010000322         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2318145,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000322 DASYLX010000000
VERSION     DASYLX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318145"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGP27_07835"
     CDS             <1..244
                     /locus_tag="VGP27_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7851260.1"
                     /translation="YVVPDGTALEKALEIAEVINKNGPLAVQAILKTIHETEGMHEND
                     AFKIDTKIGIEVFLSGDAKEGPLAFKEKRAPNFKMR"
     gene            complement(231..1274)
                     /locus_tag="VGP27_07840"
     CDS             complement(231..1274)
                     /locus_tag="VGP27_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV7851261.1"
                     /translation="MSRLDTAVAECAGLTYVTDSDPGITRERVEDGWIYRRPDGSLLC
                     GDDQAWIDALGIPPAWTDVWISPRPDGHILATGRDSRGRKQYIYHPRWREVRGRTKFH
                     RVGHFGRALPGLRRRVDHDLDLPGLSREKVLGAVLRLMDMTLIRIGNDEYARQNQHYG
                     LTTLRHDHVRFEDPTTVMFEFRAKSGKEQRVRLSDPRLANIVYACHELPGQKLFAYID
                     DAGRVVHVGSAEVNSYLREIAREIFTAKDFRTWGGTVTAADALVRIGPSRSLTEAKRK
                     INQAIDAAAERLNNTRAVCRKNYVDPRVLDSYLDGSLHLTFARIRKRPRLSRAESGVL
                     LLTDERHPHVNAS"
     gene            1348..2103
                     /locus_tag="VGP27_07845"
     CDS             1348..2103
                     /locus_tag="VGP27_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851262.1"
                     /translation="MTRVTEQGDIFFCYRPKVDAQHVRGLDDVARFYVILKPVAKARF
                     RRMIVGRKRLPAVEDHERAWGFIDLVTEEAAELEDELDPYAYETKSRGTRVEPAVRPS
                     GEGVYAIVDHGGHTHIAYALELPQTPGPVQAAFQIRPMASLIVTVRNPDAPAPPDRPR
                     AGLPPSQRPEYPDALWAAFGDRRFTKLDPPDFLDYPGTELVLIGASPDVTRELGIALP
                     TKRESRRSADVFTELGMERDVHPAEPLLTGQWR"
     gene            2207..2860
                     /locus_tag="VGP27_07850"
     CDS             2207..2860
                     /locus_tag="VGP27_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEV7851263.1"
                     /translation="MYDLDSAITERHSTRMFLRDKPVPPDLVHEALTLAMRAPSNSNI
                     QPWHLVLASGPARGRLVEALLEVAAVESPSVPALPEAFAHMRRDLGALVYGSMGISRH
                     DGDARRVAVLRNWEFFRAPLAGIVCMQRDLDYVDAIGVGMFLQTFLLALTARGLGTCV
                     QVSIAGYPDVVREQLNISEDLRILTGLAIGYPDPSFAANDLRVPRNAVRDNVVFVDR"
     gene            complement(2976..4178)
                     /locus_tag="VGP27_07855"
     CDS             complement(2976..4178)
                     /locus_tag="VGP27_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851264.1"
                     /translation="MTLTRPDVDLTDGTFYADRPAAHEAYRWMRANEPVFRDRNGLAA
                     AATYAAVIEAERNPELFSNAGGIRPDQGPLPMMIDMDDPAHLLRRKLVNAGFTRKRLK
                     VQDASIGALCDRLLDAVCERGECDLVRDIAAPLPMAVIGDMLGVLPDERELFLKWSDE
                     MMVGLSSTVSEADMQVTMDAFVAYNDYTRRKIDERRAAPTEDLISVLVHSEVDGERLS
                     DDDILQETLLILVGGDETTRHTISGGIEQLIRDREQWDQLRNNPDLLPGAVEEMLRWT
                     SPLKNMARTVTADTDFHGTSLKQGEKMILLFESANFDETVFGDPDSFSIKRDPNSHLT
                     FGFGTHFCLGNQLARIELINITRRVLQRLPDLRLAEGADLPLRPANFVSGLEQMPVKF
                     TPTKSLNI"
     gene            4252..>4606
                     /locus_tag="VGP27_07860"
     CDS             4252..>4606
                     /locus_tag="VGP27_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 family protein"
                     /protein_id="HEV7851265.1"
                     /translation="MTASRTTFAFVVLAATLALAGCGDDKPAPKASSQSSAPPAPGSP
                     IDACKLASADDIAKLLGKPVEGRPIGATPQAPACTWENRDTYESVTVEIGNPGTAING
                     TLPPPQPGFPEVGTPG"
BASE COUNT          801 a         1505 c         1485 g          815 t
ORIGIN      
        1 atacgtggtg ccggacggca cagcgctgga gaaggcgctc gagatcgccg aggtgatcaa
       61 caagaacggg ccgctcgcgg tgcaggccat tttgaagacg atccacgaga ccgagggcat
      121 gcacgagaac gacgccttca agatcgacac caagatcggc atcgaggtgt tcctctccgg
      181 cgacgccaag gaaggcccgc tggcgttcaa ggagaagcgc gcgcccaatt tcaagatgcg
      241 ttgacgtgcg ggtgccgttc gtctgtgagc aataggacgc cggactcggc gcgtgagaga
      301 cgcggtcgct tgcgaattcg cgcgaacgtc aggtgcagcg acccgtcgag atacgaatcg
      361 agcacccgag gatcgacata gttcttccgg cacaccgcgc gagtgttgtt gagccgttcc
      421 gcggccgcgt cgatagcctg gttgatcttg cgtttggctt cggtcaggga ccgggacggc
      481 ccgattcgga ccagtgcgtc cgccgccgtt acggtgccgc cccaggtgcg aaagtccttg
      541 gcagtgaaga tctcccgggc gatctcgcgc aggtaactgt tcacctcggc cgatccgacg
      601 tggactaccc gtccggcgtc gtcgatgtag gcgaagagtt tctgcccggg caactcgtgg
      661 caggcatata cgatgttggc cagccgcggg tcagacagcc gcacgcgctg ttccttgccg
      721 gacttggccc ggaattcgaa catcaccgtg gttgggtcct cgaaccggac gtggtcgtgg
      781 cgcagcgtgg ttaggccgta atgctggttc tgccgggcgt actcgtcgtt gccgatgcgt
      841 atcaacgtca tgtccatcag gcgcagcacc gcgccgagca ccttttcgcg agacagccct
      901 ggcaggtcca ggtcgtgatc aacccggcgc cgcagtcccg gcagcgcccg cccgaagtga
      961 ccaacgcggt gaaatttcgt gcggccgcgc acctcgcgcc agcgcgggtg gtagatgtac
     1021 tgctttcgac cgcgtgagtc gcgacccgtg gcaaggatgt gcccgtcggg ccgcgggctg
     1081 atccacacgt cggtccaggc cggcgggatg ccgagggcgt ctatccaggc ctgatcgtcg
     1141 ccgcagagca ggctgccgtc cgggcgccgg tatatccaac cgtcctcgac acgctctctg
     1201 gtgatgccgg gatcggaatc ggtgacatac gtcaggcctg cgcattccgc gacggcggtg
     1261 tcgagtcgac tcatccctcc gggatgccca cgtcgtcgcg acccattcac gatgtttaag
     1321 cggaagggtt gcggcgacac cacatcaatg acccgggtaa ccgaacaggg cgacatcttc
     1381 ttttgctacc gccccaaggt ggacgcgcaa cacgtgcgtg gcctcgacga cgtggcgcgt
     1441 ttctacgtca ttctcaagcc ggtcgcgaag gcgagattcc ggcggatgat cgtcggtcgc
     1501 aaacggctac ccgccgtcga ggaccacgag cgggcgtggg gcttcatcga cctcgtcacc
     1561 gaggaggcgg ccgagctcga ggacgaactc gatccgtacg cttatgaaac caagtcccgg
     1621 ggcacgcgtg tcgagccggc ggtgcgaccg tcgggcgagg gtgtgtacgc catcgttgat
     1681 cacggcggcc acacccacat cgcctatgcg ctcgagctgc cccagacgcc aggaccggtc
     1741 caggctgcgt tccagatccg gccgatggcc agcctcatcg tgaccgttcg caacccagat
     1801 gcgccggctc cgccggatcg gccgcgagcc gggctgccgc cgtcgcaaag gcccgaatac
     1861 ccggacgcgt tatgggcggc attcggcgac cggaggttca cgaagctgga cccgcccgac
     1921 ttcctcgact accccggcac cgagttggtc ctgatcggcg caagtccgga cgtcacccgt
     1981 gagttgggca tcgcgctgcc gacgaaacgg gaatcccggc gatccgcgga cgtgttcacc
     2041 gagctgggca tggaacgcga cgtccacccc gcggagccgt tgctcaccgg ccaatggcgg
     2101 taggcgaccg agatccgaat cgcgggcgag agctctcgct tccgcgagcg tgaaacctct
     2161 ggcgattttc cggccggttt tttgcagtgg gtagagactc gacgctatgt acgacctcga
     2221 ctccgcgatc accgagcgcc actcgacgcg catgttcctg cgggacaagc ccgtaccgcc
     2281 cgacctcgtt cacgaggccc tgaccctcgc gatgcgggcg ccctcgaatt cgaacattca
     2341 gccgtggcat ctggtgctgg cgtccggccc ggccaggggc cggctcgtcg aggcgcttct
     2401 cgaggtggca gcggtcgaat cgccgagcgt gccggcgctg ccggaggctt tcgcgcatat
     2461 gcgtcgcgac ctcggtgccc tggtgtacgg gtcgatgggg atttcccgtc atgatggcga
     2521 cgcccgccgc gtcgccgtgc tgcgcaactg ggagttcttt cgcgcgccgc tcgccggaat
     2581 cgtctgcatg caacgcgatc tcgactatgt cgacgcgatc ggtgtgggaa tgtttctgca
     2641 gaccttcctg ctcgcgctga cggcccgagg cctcggcacg tgcgtacaag tgtcgatcgc
     2701 gggctatccc gacgtcgtgc gggagcaact gaacatcagc gaggacttgc gaattctgac
     2761 cggcctcgcg atcggctacc ccgacccgtc gttcgcagcc aacgatctgc gagtgccgcg
     2821 aaatgcggtg cgggacaacg tggttttcgt cgaccgctga taccgcaacc tcgcgaccgc
     2881 gccagcgtgc acaaactcgc ggcctgcagc ggcgtgtcgc gcagcagaca cgcacgctcg
     2941 cggcacgagg tgaggggcgc cgcgcggggg tcgcgctaga tgttgagcga tttggtcggg
     3001 gtgaacttca ccggcatctg ttccaggccg ctgacgaagt tcgccgggcg aagcgggagg
     3061 tccgcgcctt ctgccagtct caggtccggc agccgctgca acacccgccg cgtgatgttg
     3121 atcaactcga tgcgggccag ctgattgcca aggcagaagt gggttccgaa gccgaatgtc
     3181 aagtgactat tggggtctcg tttgatgctg aagctatcag ggtcaccgaa taccgtctcg
     3241 tcgaaattcg ccgattcgaa gagcagaatc atcttctcgc cctgcttcag gctggtgccg
     3301 tggaagtccg tgtcggcggt caccgttcgc gccatgttct tcagcggcga ggtccagcgc
     3361 agcatctcct cgacggcgcc cggcaacagg tctggattgt ttcgcagctg atcccactgc
     3421 tcgcggtcgc ggatcagctg ctcgatccca ccggaaatgg tgtgacgcgt cgtctcgtcg
     3481 ccgccgacga ggatcagcag cgtctcctgg agaatgtcgt cgtcggacaa ccgctcaccg
     3541 tcaacctccg aatgcaccag gacgctgatc aagtcctccg tcggcgccgc tcggcgctcg
     3601 tcgatcttcc tgcgggtgta gtcgttgtat gccacgaagg catccatcgt gacctgcatg
     3661 tcggcctcgg acactgtgga gctgagaccg accatcatct cgtcggacca cttcaggaaa
     3721 agttcgcgct cgtctggaag cacaccgagc atgtcgccga tgaccgccat cggcagtggt
     3781 gcggcgatgt ctctgaccaa gtcacattcg ccgcgctcgc ataccgcgtc gagcagtctg
     3841 tcgcacaaag ccccgatgga agcatcctgc accttcaacc gcttacgcgt gaagccggcg
     3901 ttaacgagct tgcggcgcaa cagatgtgcg ggatcgtcca tgtcgatcat catcggcagc
     3961 ggcccttgat ccggacggat gccacctgcg ttggagaaca actccggatt gcgctccgcc
     4021 tcgatcaccg ccgcgtacgt ggcggcggca gcaagcccgt tgcgatcgcg gaacaccggc
     4081 tcgttggccc gcatccaccg gtaggcttcg tgcgcggcag ggcggtcggc gtaaaacgtg
     4141 ccgtcggtca gatcaacgtc tggtctggta agggtcatgc ctgcctccat cggttgataa
     4201 gggaatctag acgacgcgtc aacaggcgga tctgcattac cgtgaacccg catgaccgcc
     4261 agcagaacca cattcgcatt cgtcgtgctc gcggcgacac ttgccctcgc cgggtgtggc
     4321 gacgacaagc cggccccaaa agcgtcgtcg cagtcgagcg cgccgcccgc ccctggctcg
     4381 cccatcgatg cgtgcaagct ggcaagcgcg gacgacatcg ccaagctgct cggtaaaccg
     4441 gtcgaaggca ggccgatcgg ggcaaccccc caggcacccg cctgcacgtg ggagaaccgc
     4501 gacacctacg aatcggtcac agtcgagatc ggcaaccccg ggacggcgat caacggcacg
     4561 cttccgccac cccagccggg tttccccgag gtcggcacgc ctggcc
//
LOCUS       DASYLX010000323         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2646194,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000323 DASYLX010000000
VERSION     DASYLX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646194"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGP27_07865"
     CDS             <1..808
                     /locus_tag="VGP27_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851266.1"
                     /translation="LVSETKDATGEQVHVQTMATNSHRLPAYDPTGLSTGVLWQHADR
                     DVKLESESAQDRARRASAAREELARRIGGQASAVVVGHLAGGKPRATRGAAGEDAAGH
                     GAGHINARHVIGQSRIDTAPPALGTTVTPDWLAYRAVTNNPPCGGMAGGFANAGAAAA
                     GIQAAATQLTIDWPAFRDRLIVNGRVQEIVPVAGAGICFLRTANTAAAQLLPAWLGGP
                     TNRKLHAQDTRATDEPPLADSSAPSGTELRILLSPTSPNGWFVHSAWPTI"
     gene            811..1197
                     /locus_tag="VGP27_07870"
     CDS             811..1197
                     /locus_tag="VGP27_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851267.1"
                     /translation="MTSGEDSEREGRRLLELIEQNRVAHRRHPDRFLAEISADLWRNM
                     PDDEALDDFRHRLRTAPWWAIDAVECLEAVVRDRPEWAARTFAEASDRWLGASADDDP
                     DMYLDWLAERTRAMRRAVDDEARGSL"
BASE COUNT          314 a          596 c          607 g          216 t
ORIGIN      
        1 cctggtgtca gagaccaagg acgcaacggg cgaacaggtg cacgtccaga cgatggccac
       61 caactcgcac cgattgcccg cgtacgaccc gaccggcctg tccacgggcg tgctctggca
      121 gcacgccgac cgggacgtga agctcgagag tgagtcggcg caagaccggg cccggcgggc
      181 gagcgccgcc cgggaagagc tcgcgcgccg gatcggcggc caggcaagtg cggtggtggt
      241 gggccatctc gccggcggaa agccgcgggc gacgcgggga gctgccggcg aggacgccgc
      301 gggacacggc gcgggacaca tcaacgccag gcacgtgatc ggccagtccc ggatagacac
      361 cgcgccgccc gccctgggca ccacggtgac gccggactgg ctcgcgtatc gagcggttac
      421 caacaacccg ccctgcggag gcatggccgg cgggttcgcg aacgccggcg cagcggcggc
      481 agggatccag gccgcggcga cacagctgac gatcgactgg ccggcgttcc gcgaccggct
      541 catcgtgaac ggaagggtcc aggagatcgt gccggtggcc ggcgcaggca tctgcttcct
      601 ccgcactgcg aacacggctg ccgcgcaact actcccggcg tggctgggcg gcccgaccaa
      661 ccggaagctc catgcccagg acacccgcgc gactgacgag ccgccgctcg ccgacagcag
      721 cgcgccgagc ggcaccgagc tgcgtatcct gctcagcccg acctcgccga acggctggtt
      781 tgtgcacagc gcgtggccga caatctgacc atgacctcag gagaggacag cgagcgcgag
      841 ggcagaaggc tgctcgaact gatcgagcag aaccgcgtgg cccatcggcg ccatcccgat
      901 cgattccttg ccgagatctc ggccgatctg tggcggaaca tgccggacga cgaggcactc
      961 gacgactttc ggcatcgact gcggacggca ccgtggtggg ccatcgacgc ggttgagtgc
     1021 ctcgaggccg tggtccggga ccgtccggag tgggcagcgc ggacgtttgc cgaggcgagc
     1081 gatcggtggc tgggggcgtc ggccgacgac gacccggaca tgtacctcga ctggctcgcc
     1141 gagcgaaccc gcgcgatgcg acgcgcagtc gacgacgaag cgaggggaag cctctgaagc
     1201 cgcggagtgg cagagggcgc ccgagggaga ggccgacgtc cggcctgcaa gatgatcgtc
     1261 gacatcgtca cgttcatcgt gacccgcggc gcgcagatcc cgcagttcgt caacgccgtc
     1321 atcgcggtat gccaccggcg gcataccggc gctgatcgag aacgggctca ccaagagcat
     1381 cccggtcctg atcggcgccc tcgccgccat cctcggcatc ggcggcatcg ccgagaaggt
     1441 ctagaagttc ttccaggctc tcgccaaacc ggtcatgaag gccgtcgact gggtcgtcga
     1501 caagatcgtc gccgtcggca agaagatctg ggcgaactga agagcaagtt cggcagagcc
     1561 ggggatacga agtacgacag catcatctgg ctctacccca ccccggcggc ccacggtccc
     1621 agaccgccgc cagcggggca gcgctcctgg ccgcattctt cgccagcgcc gcacagaggc
     1681 tggcacccgg cggaaagatc tcggtcacct tgcgcgtcgc caagaacccg acc
//
LOCUS       DASYLX010000324         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_123694,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000324 DASYLX010000000
VERSION     DASYLX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123694"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VGP27_07875"
     CDS             complement(<1..930)
                     /locus_tag="VGP27_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7851268.1"
                     /translation="MSESERSESYGTPMSSDDAAELEELRREAAVLREQLENAVGPQS
                     GLRSARDVHQLEARIDSLAARNSKLMETLKEARQQLLALREEVDRLGQPPSGYGVLLG
                     VQEDDTVDVFTSGRKMRLTCSPNIDTTSLKQGQTVRLNEALTVVEAGNFESVGEISTL
                     REILADGHRALVVGHADEERIVWLADPLVAAEDLPVSYDDDVDDDLRPRKLRPGDSLL
                     VDTKAGYAFERIPKAEVEDLVLEEVPDVSYGDIGGLGRQIEQIRDAVELPFLHKELYR
                     EYSLRPPKGVLLYGPPGCGKTLIAKAVANSLAKK"
     gene            1213..1512
                     /locus_tag="VGP27_07880"
     CDS             1213..1512
                     /locus_tag="VGP27_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851269.1"
                     /translation="MLDTLVEPGLKFNSPAKENLVQGGVGPLKGRLADGALKNAYADG
                     TLPTDITVDPPTCIDPATATSTVRAAGKSMPITFVNEGGWKVSAASASTVLAAFA"
     gene            1551..2048
                     /locus_tag="VGP27_07885"
     CDS             1551..2048
                     /locus_tag="VGP27_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851270.1"
                     /translation="MVVFAFFGVIALSGCNSDTPPAAEPSMTTAVAAEPPPVSSPLPP
                     PEALIDVLARLSDPAVPGAEKTGLVELATAGDAAALDRFGKALADNGALPLSFEAVDM
                     KWSDTEAGNVVATVNVTTANKPPGTFTFPMEFTPLRTGWQLTRKTADLLLQFGEGATA
                     PSPPP"
     gene            2060..2356
                     /locus_tag="VGP27_07890"
     CDS             2060..2356
                     /locus_tag="VGP27_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HEV7851271.1"
                     /translation="MWIGWLEFDLLLGDVHSLKQKRSAVRPVIAELQRRFAVSASETG
                     SQDLHRRAGIGMAVVSGDRTHVVDVLDAAERLVAARPEIELLSVRRGLRRSDDD"
     gene            complement(2353..3180)
                     /locus_tag="VGP27_07895"
     CDS             complement(2353..3180)
                     /locus_tag="VGP27_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEV7851272.1"
                     /translation="MPRTGPFAVGDRVQLTDVKGRHYTVVLTTGGEFHTHRGAIAHDK
                     VIGLPEGSVVPSANGDPFLVLRPLLVDYVMSMPRGAQVIYPKDAAQIVHEGDIFPGAR
                     VLEAGAGSGAMTCSLLRAVGPEGRVVSYDVRDDHAQHASRNVATFFGELPENWDLVIA
                     DVAEYSGPQVDRVVLDMLAPWEVLGTIAEALVAGGVLMIYVATVTQLSRVVEALREQQ
                     CWTEPRAWETLQRGWHVVGLAVRPQQSMRGHTAFLISARRLAPGTVAPMQSRRKRQI"
     gene            3255..>3503
                     /locus_tag="VGP27_07900"
     CDS             3255..>3503
                     /locus_tag="VGP27_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEV7851273.1"
                     /translation="MDQNEVPSPPSEHRRPALSPSRASDFKQCPLLYRFRAIDRLPEP
                     ASTAQLRGSVVHAALEQLYALPAPDRVPETARSLVDPAW"
BASE COUNT          561 a         1195 c         1131 g          616 t
ORIGIN      
        1 cttcttggcc agcgagttgg cgaccgcctt ggcgatcagc gtcttgccgc agccgggagg
       61 tccgtagagc agcacacctt tcggcgggcg cagcgagtac tcccggtaaa gctccttgtg
      121 caggaaaggc agctcgaccg cgtcgcggat ctgctcgatt tggcgaccca ggccgccgat
      181 gtcgccgtag ctcacatcgg gaacctcttc cagcaccagg tcctcgacct cggccttggg
      241 gatgcgctcg aaggcgtagc cggccttggt gtcgaccagc agcgagtccc cgggtcgcag
      301 cttgcgcggc cgcaggtcgt cgtcgacgtc gtcgtcgtag ctgacgggca ggtcctccgc
      361 cgcgaccagc ggatcggcga gccagacaat gcgttcctcg tcggcgtgac cgaccacgag
      421 ggcgcggtgc ccatcggcaa ggatttcgcg cagggtggag atttccccga cggattcgaa
      481 gttcccggcc tccacgacgg tcagcgcctc gttcagacgg acggtctggc cctgcttcag
      541 tgacgtcgta tcgatgttcg gcgagcacgt cagccgcatc ttgcggccgg aggtgaacac
      601 gtcgacggtg tcgtcctcct ggacgccgag gagcacgccg taaccgctcg gcggctgacc
      661 cagccggtcg acctcctcac ggagggcgag caactgctgg cgggcctcct tgagggtctc
      721 catcaacttg gaattcctgg cagcgagcga atcgatcctc gcctccagct ggtgcacatc
      781 acgggcgcta cggagaccgc tctgcggtcc caccgcgttt tccagctgct cgcgcagtac
      841 tgctgcttct cggcgaagtt cctcgagctc ggccgcgtca tcgctggaca tcggtgtccc
      901 gtaggattca gaacgctctg actcgctcat gttgcgctcc ctccatgcac cgcaagttgg
      961 tgcagtaaca acttcaacgc taccggcgat tcagcctttg tgtgcggtct aggtattcga
     1021 cacaccagca ccacttgtta gcctgaagcc tgacccgggc caatcgaaag gacaatcgtg
     1081 accctcaaaa ccctccttac tggcgttgca gctgctgctg tgatgggtgg tgcggccatg
     1141 ggtgtgacat cgattgcatc ccccgcaatc gcttccgcag acgactgcgt ctcccccgcc
     1201 gcactgaagg gtgtgctcga cacgctcgtc gagcctggtt tgaagttcaa ttcacctgcc
     1261 aaggagaatc tggttcaggg tggtgttggg ccattgaagg gccggttggc cgacggcgcg
     1321 ctgaagaacg cgtacgccga cggcacgctg ccgacggata ttaccgtgga cccgcccacc
     1381 tgcatcgatc ccgctaccgc gacctcgact gtccgcgcgg cgggcaagag catgccgatc
     1441 accttcgtga acgaaggcgg gtggaaggtc tccgccgcct cggcgtcgac ggtgctcgca
     1501 gcgttcgcct gaccccgacc aacatgcgac agcggccaac ggtatttccg ttggtcgttt
     1561 tcgcgttctt cggcgtcatc gcactgtcgg ggtgcaacag cgatacgcca ccggcggccg
     1621 aaccgtcgat gacgacggcc gtcgcggccg aaccgccgcc cgtgtcatct cccctcccac
     1681 cgccggaggc actcatcgat gtgctggccc ggctgtcgga tcccgctgtg cccggcgcgg
     1741 agaagaccgg cctcgtcgag ttggcgaccg ccggtgacgc cgcggcgctc gacaggttcg
     1801 gcaaggcgct cgccgacaac ggcgctctac cgctgagctt cgaggcggtc gacatgaagt
     1861 ggtcggacac cgaagcggga aacgtggtgg cgaccgtcaa cgtcacgacc gccaacaagc
     1921 cgcccggcac gttcaccttc ccgatggagt tcacccccct ccggaccgga tggcagttga
     1981 ctcgcaagac cgctgatctg ctgctccagt tcggcgaggg ggcaaccgcc ccatcgcctc
     2041 ctccctgacg atggccggta tgtggatcgg ctggctcgag ttcgacctgc tgctcggtga
     2101 cgtgcattcg ctgaaacaga agcgctctgc ggttcggccg gtgatcgccg aacttcaacg
     2161 gagattcgct gtttcggcgt cggaaaccgg atcgcaggat ctgcaccggc gcgccggtat
     2221 cgggatggct gtggtgtcag gcgaccggac tcacgtggtg gatgtgctcg atgccgccga
     2281 gcgattggtc gccgcacggc cggagataga actgctctcg gtgcggcgcg gactgcgacg
     2341 cagcgacgac gactagatct gtcgcttacg ccgtgactgc atcggcgcga cggtgccggg
     2401 tgcgagcctg cgcgcgctga tcagaaacgc tgtgtgtcca cgcatgctct gttgcggccg
     2461 cacggccagc cccaccacgt gccagcctcg ctggagcgtc tcccacgccc gcggttcggt
     2521 ccagcactgc tgctcccgca atgcctcgac gacccgcgac agctgggtca ccgtcgccac
     2581 gtagatcatc agtacgccgc ccgccaccag cgcttcggcg atcgtgccca gcacctccca
     2641 tggggccagc atgtccagca cgacgcggtc cacttggggg ccgctgtatt cggcgacgtc
     2701 ggcgatgacc aaatcccagt tctcgggcag ctcgccgaag aaggtcgcga cgttgcgcga
     2761 cgcgtgctgg gcgtggtcgt cacgcacgtc gtaggacacc acccggccct cggggccgac
     2821 ggcgcgcagc agcgagcacg tcatcgcacc cgagcctgcg cccgcctcca acaccctggc
     2881 gccgggaaag atgtcgcctt cgtgcacgat ttgagccgca tccttcgggt agatcacctg
     2941 cgcaccgcgc ggcatcgaca tcacgtagtc gacgagcagg ggacgcagca caaggaacgg
     3001 atcgccattg gccgacggga ccacgctgcc ctccggcagt ccgatcactt tgtcgtgggc
     3061 gatcgctcct cggtgggtgt ggaattcgcc gcctgttgtc aggaccacgg tgtagtgccg
     3121 ccccttgacg tcggtgagct gcacgcgatc tccgacggcg aacggaccgg tccttggcac
     3181 agctgcccag cctgccagca gacgcgccga gggaggtgtc cggggttgtc ggagcggagc
     3241 cataagctgc cgtcatggac cagaacgagg tcccgtcccc gccatcggag caccgaaggc
     3301 cggcgctgtc gccgtcgcgg gccagcgact tcaagcagtg tccgctgctg tatcggttcc
     3361 gggccatcga ccgcctgccc gagccggcgt cgaccgcgca gctgcggggg tcggtcgtgc
     3421 acgcggcgct cgaacagctc tacgcactgc ctgcgcccga tcgtgtgccc gagaccgcga
     3481 ggtcgttggt cgaccccgcg tgg
//
LOCUS       DASYLX010000325         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_452746,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000325 DASYLX010000000
VERSION     DASYLX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452746"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VGP27_07905"
     CDS             complement(<1..1239)
                     /locus_tag="VGP27_07905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851274.1"
                     /translation="MSKSALAITDEHQDLADAANGQLARLSARAAARATLEGGSSRPD
                     NVWKAAAELGWLGLAVAEEHGGSGFGLAELAVVLEAQGRELSPGPFLPTVAAAVTIDR
                     CASDSLRAELLPGLTDGTTVGALGLSGSVVVGSDLDVTGECPSVLGAPDADVLVLVAG
                     DDVVVVDAAADGVTVSSLEALDTSRSVGSVALRGVNVTGDRVIRGAARRARTVFRILA
                     SAEAVGVAWATLEMAVEYAKVREQFGRTIGTFQAVKHHAANMLVDAEQTTAAVWDAAR
                     AEDLDSAWFPAAVAASHAIRAQVFNAQNNIQLHGGIGFTWEHDAHLYLRRARTLAALI
                     GDGADPLLDVVDGRRTGQAHGASFTLPAEADDYRRQAAEVVEQVRSLPEDKQRDFLVD
                     SGYLVPHWPTPWGRAADVLEQ"
     gene            1403..2626
                     /locus_tag="VGP27_07910"
     CDS             1403..2626
                     /locus_tag="VGP27_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851275.1"
                     /translation="MTVRPDGRFSAPALRKRARWFLRAGPTDYLLAMSVASASLPIIG
                     KRLEPLGAATAMGIWGYKHVPDLFSKSARPRDISDQTDNRYRDLESTNAVIDAALRGT
                     VSAADLTIDWPVPERHAPVLKMRQHRRSLHRASVRYGDHPSQLLDVWRVKELPVEPAP
                     VMIFVPGGAWIHGGRQLQGYALMSHLAQLGWVCLSVNYRVAPHHRWPAHITDVKTAIA
                     WARANVDRFGGDRNFVAIAGCSAGGHLSALAGLTPNDPELQADLPKGSDTSVDAVVGI
                     YGRYDWEDRSTPERDRFVDFLERVVVQRKIDRHPEIFRKASPIAAIHPDAPPFLVIHG
                     SGDSVIPVAQARSFVERLRAVSRSVVSYIELPGAGHGFDMTDGARTGSVATAIGLFLN
                     HIHRNRSMMKAKEVI"
     gene            2652..>3815
                     /locus_tag="VGP27_07915"
     CDS             2652..>3815
                     /locus_tag="VGP27_07915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7851276.1"
                     /translation="MKRLSGWDAVLLYSETPTVHMHTLKLAVIEIGDLKGRKFGIEEF
                     REVIRGRLPKLEPFCYQLVDIPLKLHHPMWRENCEVDLDYHVRPYSVDSPGGRRQLDD
                     AVGRIASTALDRSKPLWEMYFIEGLANGRIAVLGKIHHALADGVASANLLARGMDLRE
                     GPPPERHYANDPAPSKGELVRSAFADHARHIGRLPGVMRYTAQGLGRVRKSSKKFSAE
                     LTRPFTPPPTFMNHKVDADRKFATATLALADVKETAKHLGVTINDMVLAISAGALRGL
                     CLKYDGRADHPLLASVPVSFDFSRDRVSGNYFSGVMMVLPIELDDPLERVQAVHDAAV
                     DAKETHHLMGPELVSRWASYFPPAPAEALFRWLAEKDGQNKVLNLPISNVPGPR"
BASE COUNT          648 a         1268 c         1252 g          647 t
ORIGIN      
        1 ctgctcgagg acgtcggccg cccgacccca tggagttggc cagtgcggca ccagatatcc
       61 ggagtcgacg aggaagtcgc gctgcttgtc ctcgggtagc gaccgcacct gctccacgac
      121 ctcggcggcc tgacggcggt agtcgtcagc ctcggcgggc agtgtgaacg aggcgccgtg
      181 ggcctggccg gtgcgcctgc cgtccacgac atcgagcaat gggtcggcgc cgtcgccgat
      241 cagcgccgcc agggtgcggg cccggcgaag gtaaagatgg gcatcgtgtt cccaggtgaa
      301 accgatgcca ccatgcaact ggatgttgtt ctgcgcgttg aaaacctgcg ccctgatagc
      361 gtgtgacgcc gccaccgcgg cggggaacca tgcgctgtcc agatcctcgg cgcgggccgc
      421 atcccagacg gccgcggtgg tctgttccgc atcgacgagc atgttggcgg cgtggtgctt
      481 cacggcctgg aaggtgccga tggttctgcc gaactgctca cggaccttgg cgtactccac
      541 cgccatctcc agggtggccc aggccacgcc gaccgcctcc gccgacgcca gaatacgaaa
      601 caccgtgcgg gcccgccgcg cagcgccgcg tatcactctg tccccggtga cgttgacgcc
      661 gcgcagtgcg accgacccga cactgcggga ggtgtcgagg gcttccagcg aactgaccgt
      721 gacgccgtcg gccgcggcat cgacgaccac cacgtcgtcg ccggcgacaa gcacgagcac
      781 gtcggcatcg ggcgcaccga ggaccgacgg acactccccg gtgacgtcga ggtccgagcc
      841 gacgaccacg ctgccggaca gaccgagcgc cccgaccgtc gtcccgtcgg tcaggcccgg
      901 aagcagttcg gcgcgaagtg aatcggatgc acagcggtcg atcgtgacag cggccgccac
      961 cgtcggcaga aacgggccgg ggctcagctc ccggccttgg gcctccagca ccaccgccaa
     1021 ctccgccaga ccgaaacccg aaccgccgtg ctcctcggcg acggcgagcc ccagccagcc
     1081 cagttctgcc gccgccttcc atacgttgtc gggacgggac gacccaccct cgagcgtggc
     1141 gcgtgcggcg gccctggccg acagcctcgc caattggccg ttcgcggcgt cggccaggtc
     1201 ttggtgttcg tcggtgatgg ctagcgctga cttgctcacg ttggaagtcc tttgccgttg
     1261 acgggtgtct agaggcagtc aacctgcatt ctggcaggtc ggcatttgtg atgtggatca
     1321 cattttggct cacaacgtgc gataagtaga caaccctcga aagttgtcgt ttcacggacg
     1381 cgttgcgggg tacattttgg aaatgacggt caggccggac ggccggttct ccgcgcccgc
     1441 tttacggaaa cgcgcccgct ggtttctccg cgcagggccc accgattatc tgctggcgat
     1501 gagtgtcgcg tctgcgtcac tgcccattat cgggaaacgc cttgagcccc ttggtgctgc
     1561 gaccgcgatg ggcatctggg gatacaagca cgtaccggat ctcttctcaa agagcgcacg
     1621 cccgcgggat atctccgacc agacggacaa tcggtaccga gacctcgagt cgaccaacgc
     1681 cgtgatcgat gccgcgctgc ggggcaccgt gtccgccgcc gacctgacca tcgactggcc
     1741 ggtgccggag cggcacgcgc cggtgttgaa gatgcggcag caccgccgca gcctgcaccg
     1801 cgcctccgtt cgctacggcg atcacccgtc acagttgctg gatgtgtggc gcgtcaagga
     1861 attgcctgtc gagccggcac cggtgatgat cttcgtgccc ggcggcgcat ggatccatgg
     1921 cggacgacaa ctgcagggct acgcgctgat gtcgcatctg gcccagctgg gctgggtatg
     1981 cctgtcggtg aactaccggg tggcaccgca ccatcgctgg cccgcacaca tcaccgacgt
     2041 gaagaccgcc atcgcgtggg cccgcgccaa cgtcgaccgc ttcggcggcg accgcaattt
     2101 cgtcgcgata gcgggttgtt cggcgggtgg ccacctctcg gcgctggccg gtctgacgcc
     2161 taacgatccc gagttgcagg ctgacctccc gaagggctct gacacctccg tcgatgcggt
     2221 cgtcggaata tacggccgct acgactggga ggaccgttcg accccggagc gcgaccgctt
     2281 cgtcgacttc ctggagcgcg tggtggtcca gcgcaagatc gaccgtcacc cggagatctt
     2341 ccggaaggcc tcgccgatcg ctgcgatcca tcccgatgcc ccgccgttcc tggtcatcca
     2401 cggcagtggc gactcggtga tcccggtggc tcaggcgcgt agcttcgtcg agcggttgcg
     2461 cgcagtatcc cgttcggtgg tgagctacat cgagttgccg ggtgcaggcc acgggttcga
     2521 catgaccgac ggcgcccgca cgggttcggt ggctacggca atcggcttgt tcctcaacca
     2581 tattcaccga aaccggtcga tgatgaaagc caaagaggtt atatagacgc ctccggtagg
     2641 aggtatcagg ggtgaagagg ctcagcggct gggacgcggt actgctgtat tccgagacgc
     2701 caactgtgca catgcacaca ctcaagttgg cggtcatcga gatcggcgat ctgaagggcc
     2761 gaaaattcgg catcgaggag ttccgtgagg tcatccgcgg ccgcctgccc aagctcgagc
     2821 cgttctgcta tcagcttgtc gatattccgc tcaagctgca ccacccgatg tggcgggaga
     2881 actgcgaggt cgacctcgac taccacgtcc ggccgtacag cgtcgacagc cccggcggcc
     2941 gccgccagct ggacgacgcg gtcggtcgga tcgccagcac cgcgctggac cgcagcaagc
     3001 cgctgtggga gatgtacttc atcgagggtc tggccaacgg ccgcatcgcg gtgctgggca
     3061 agattcacca tgcgctggcc gacggtgtcg cgtcggccaa tctgctggcg cgcggaatgg
     3121 acctacggga ggggccgccg ccggaacggc actatgcaaa tgaccccgcc ccaagcaagg
     3181 gcgagctggt gcgctcggcg ttcgccgacc acgcgcggca catcggcagg ctacccggcg
     3241 tgatgcggta caccgcccag ggtctgggac gcgtgcgcaa gagctccaag aagttctcag
     3301 ccgagctcac ccgtccgttc accccgccac cgaccttcat gaaccacaag gtcgatgcgg
     3361 accgcaaatt cgcaaccgcg actctggctc tggcggatgt gaaggaaacg gccaagcacc
     3421 tcggcgtcac gatcaacgac atggtgctcg cgatctcggc cggggcgcta cgcgggctgt
     3481 gcctgaagta cgacggtcgg gccgaccatc cgttgttggc ctccgtcccg gtgagtttcg
     3541 acttttcgcg agatcgcgtc tcgggcaact acttcagcgg tgtgatgatg gttttaccaa
     3601 tcgagctcga cgatccgctg gagcgggtgc aggcggtgca tgacgccgcg gtcgacgcca
     3661 aggagaccca ccatctgatg gggcccgagc tggtcagccg atgggcctcg tatttcccgc
     3721 cggctccggc ggaggcgcta ttccgctggc tcgcggagaa ggacggtcaa aacaaggtgc
     3781 tcaacttgcc catctcgaac gtgccgggac cgcgt
//
LOCUS       DASYLX010000326         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3415493,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000326 DASYLX010000000
VERSION     DASYLX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415493"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VGP27_07920"
     CDS             complement(<1..734)
                     /locus_tag="VGP27_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7851277.1"
                     /translation="MARTHRVVDHIVAYLAASGVEYIFGVDGANIEDVYDAAHFCDGI
                     TAVLAKHEFSAATMADGFSRSGAGLGVVAATSGGGCLNTVAGLGEALASRVPVLALIG
                     QPPTTLDGRGAFQDTSGRNGALNGEALFSAVSVFCRRISAPEEIATALPQAIAAARRG
                     GPAVLLLPKDIQQAALEFGKSVNRPTNGVAVRRQLGDQQVLARALRRADGPITIIAGE
                     QVARDDARAELAQLRSVLRARVATVP"
     gene            complement(805..1539)
                     /locus_tag="VGP27_07925"
     CDS             complement(805..1539)
                     /locus_tag="VGP27_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV7851278.1"
                     /translation="MTQPASRDDDLRGNDPYATWDAAYVLGSLSGAERREFEAHLASC
                     PRCSATVAELSGIPALLAQLSAEEVEALDDPTTAETPALPTEVLDSLLQKVQWRRRRT
                     RRRSVITLAAAAAVLAVALVVGILPGIRGHGDNTEMASAITMSKVAQTPINAAITMTG
                     FGWGTRIDMVCTYGDYASRGDATTENLSMVLLDHNGSQSEIATWVGVNGATALPSGNT
                     TMQKDQIKSVQLVDANTRAVLLQTDL"
     gene            complement(1536..2024)
                     /locus_tag="VGP27_07930"
     CDS             complement(1536..2024)
                     /locus_tag="VGP27_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7851279.1"
                     /translation="MRVLYQEHGPALWRYAVRLTGDPARAEDVVQETLLRAWQHPEVT
                     DGDRPARAWLFTVARNMIIDERRSARFRNEAGTPDPEKTYDRAGPDEVDSALDRMLLS
                     GALAQLSAEHRAVVKRSYYQGWTTAQIAADLDIAEGTVKSRLHYAVRALRLTLQEMGV
                     TR"
     gene            complement(2128..2925)
                     /gene="map"
                     /locus_tag="VGP27_07935"
     CDS             complement(2128..2925)
                     /gene="map"
                     /locus_tag="VGP27_07935"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV7851280.1"
                     /translation="MITVPGLRTRKVVDPRTPGELDAMAAAGALVAAALHAVRKAAAP
                     GVSTLELDNVAESVIRDACGTPSFLGYHGFPASICSSVNDRVVHGIPVATEVLAAGDL
                     VSIDCGAILDGWHGDSAITFGIGALIGVDEALSEATRESMEAGIAAMVPGNRLTDVSH
                     AIETATRAAEKRYDRRFAIVAGYGGHGIGRRMHMDPFLPNEGQPGRGPHLVAGSVLAI
                     EPMLTLGTSKTVVLEDEWTVVTEDGSRAAHWEHTVAVTDGGPRILTA"
     gene            complement(2929..3477)
                     /locus_tag="VGP27_07940"
     CDS             complement(2929..3477)
                     /locus_tag="VGP27_07940"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV7851281.1"
                     /translation="MRVVLLGPPGAGKGTQAQKLSEKLGIPQISTGDLFRENISSGTP
                     LGLEAKRYLDAGDLVPSELTNKLVEDRIEQSDAVDGFILDGYPRSVAQAEALDEMLKN
                     HNTKLDAVLEFAVSEEELFSRLKARGRADDTEDVIHNRMKVYSDETRPLLEYYSHNNL
                     QTVDAVGGLDEVFARALRSLGK"
     gene            complement(3474..4799)
                     /gene="secY"
                     /locus_tag="VGP27_07945"
     CDS             complement(3474..4799)
                     /gene="secY"
                     /locus_tag="VGP27_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV7851282.1"
                     /translation="MLSAFISSLRTADLRRKILFTLAILILYRVGASIPSPGVNYGNV
                     EKCLTQLSGGDSAQIYSLINLFSGGALLKLAVFAIGIMPYITASIIVQLLGVVIPRFE
                     ELRKEGQAGQAKLTQYTRYLSVALALLQATSIVALAANGGLLQGCSLDIIQSTSIFSL
                     AVIVLVMTAGATLVMWMGELIQERGIGNGMSLMIFASIAARIPSEGKSILDSRGPVVF
                     TAVCFAALVIIVGVVFVEQGQRRIPVQYAKRMVGRKMYGGTSTYLPLKVNQAGVIPVI
                     FASSLIYIPHLITQLIQSGQATPGNGWWDKLVANYLTNPASPSYIAIYFGLIVFFTYF
                     YVSVQFNVDERADEMKKYGGFIPGIRPGKPTADYLRFVLNRITLPGSIYLGVIAVLPN
                     LFLEIGNSGGVQNLPFGGTAVLIMVGVGLDTVKQIESQLMQRNYEGFLK"
     gene            5107..6036
                     /locus_tag="VGP27_07950"
     CDS             5107..6036
                     /locus_tag="VGP27_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851283.1"
                     /translation="MARTDNDTWDLASSVGATATMVAAARAMGTRIDGALISDPYAEP
                     LVRAVGVDVFTRLATGELDPSVFNIDDDTNAGMDRMVDNMAVRTKFFDDFFTDATAAG
                     VRQVVILASGLDSRAYRLQWPAGTVVYEVDQPEVIAFKTRALAEIGAEPTCDRRVVAI
                     DLRFDWPAALKEAGFDPDQPTAWSAEGLLGYLPAEAQDRLLDTMTELSAPGSWLATES
                     TPPINQDDADEAMKKMQTAAERWREHGYDLDWANLVYLGDRNEPAAYLTERGWNTDGS
                     NVNELLTANGFPPFDGDTPFADLRYISGTFGNH"
BASE COUNT         1050 a         1952 c         1966 g         1069 t
ORIGIN      
        1 tcggggacgg tggccacgcg ggcgcgcagc acggaacgca gctgcgcgag ttcggcgcgt
       61 gcatcgtcgc gggcgacctg ctcaccggcg atgatcgtga tcggaccgtc ggcgcgcctc
      121 agcgcccgtg cgagcacctg ctgatcgcct agttgacgtc gaaccgcgac accgttggtg
      181 ggtctattca ccgatttacc aaattcaagg gcggcctgct gaatgtcctt cggcagaagc
      241 agcacggccg gcccccctcg tctggcggcc gcgatggcct gcggcagcgc cgtggcgatc
      301 tcctcgggcg ccgaaatccg acggcagaac accgaaaccg cggagaacag ggcctcaccg
      361 ttcagcgcgc cgtttcggcc gctggtgtcc tggaaggcgc cgcggccgtc gagagtcgtc
      421 gggggttggc cgatgagcgc cagcaccggc acccggctgg caagcgcctc accaagcccg
      481 gcaactgtgt tcaggcagcc gccaccggag gttgcagcca ccacgccgag tccggcaccg
      541 ctgcggctga acccgtcggc catggtcgcc gcggagaact catgtttggc cagtacagcg
      601 gtgatgccgt cacagaagtg cgcggcgtcg tagacatcct cgatgttcgc gccgtcgacg
      661 ccgaagatgt actcgactcc gctcgccgcc agataggcga caatgtgatc caccacccga
      721 tgcgttctgg ccattcattc acctgctctc aaccctgcag atgacacgac cctcgggcga
      781 ccacggttca atccgtgacc ggactcacag atctgtttgc aacagcaccg ctcgtgtgtt
      841 ggcatcgacg agttgcacgg acttgatctg gtccttttgc atcgtcgtgt tcccgctcgg
      901 caacgcggtg gcgccgttca cgcccaccca cgtagcgatc tcgctctgag atccgttgtg
      961 gtccaacaga accatcgaca gattctccgt cgtcgcatcg ccgcggctcg catagtcgcc
     1021 ataggtgcag accatgtcga tccgggtgcc ccagccgaat cccgtcatcg tgatggcggc
     1081 gttgatcggc gtctgcgcga ccttgctcat cgttatggcg gacgccatct cggtattgtc
     1141 gccatgcccg cggataccgg gcaggatccc gacgaccagc gcgacggcca acaccgcggc
     1201 ggcggcggcc aacgtgatca cactgcgcct gcgggtgcgg cggcgtcgcc actggacctt
     1261 ctgcagcagt gagtcgagga cctcggtagg cagcgccggt gtttccgccg tcgtcgggtc
     1321 gtcgagtgct tccacttctt cggcggacaa ctgagccaac aacgcgggga tgccgctcag
     1381 ttcggcgacg gtcgcgctgc atcgcgggca ggaggcgagg tgcgcttcga actcccgccg
     1441 ttcggccccc gacagcgaac ccaggacgta ggcggcatcc cacgttgcgt acgggtcgtt
     1501 gccgcgcaga tcgtcatcgc gactcgcagg ttgcgtcatc gggtcacccc catttcctgc
     1561 agtgtgaggc gcagcgcgcg cacagcgtaa tgcagcctcg atttaactgt gccttcggca
     1621 atgtcaagat cggcagcgat ctgggcggtc gtccagccct ggtaatacga ccgcttgacc
     1681 accgctcggt gttctgcgga taactgtgcc agggccccgc tcaacaacat tcggtccaag
     1741 gcggaatcca cctcatccgg gccggcgcgg tcgtaggtct tttccgggtc cggggttccg
     1801 gcctcgtttc gaaacctggc gctgcgccgc tcgtcgatga tcatattgcg cgcgacggtg
     1861 aacagccagg ctctcgccgg acggtcacca tcggtcactt ctgggtgctg ccacgcccgc
     1921 agcaacgttt cttgcactac atcctctgct cttgccgggt caccggtaag ccgcacggca
     1981 tagcgccata atgctgggcc gtgttcttgg tagagcactc gcattagcgc gacttccgga
     2041 tcgtccacta caacctcccc cgttaatacg aggccgaggc cgatcgggtt cacctgatct
     2101 gcccgaccaa ctgggcagtc gggagattca ggccgtcaga atccggggtc cgccgtcggt
     2161 gacagcaaca gtgtgctccc aatgcgcggc gcgcgacccg tcttcggtca caacggtcca
     2221 ctcgtcctcg agcacgacgg tcttcgacgt gccgagggta agcatcggtt cgatcgccag
     2281 cacggagccg gcaaccaggt gcggccctcg ccccggctgg ccctcgttcg gcagaaacgg
     2341 gtccatatgc atgcgcctgc ctatgccgtg gccgccgtag cctgcgacga tagcgaatct
     2401 gcggtcgtag cgcttctcgg ctgcccgggt ggcggtctcg atggcgtgcg acacatcggt
     2461 gagccggttt ccgggcacca tggcggcgat gcctgcctcc atcgactccc tggtggcctc
     2521 cgacagcgcc tcgtcgactc cgatgagcgc cccgatcccg aaggtgatcg ccgaatcgcc
     2581 gtgccagccg tcgaggatgg caccgcagtc gatggacacc aggtcaccgg ccgcgaggac
     2641 ctcggtggca accgggattc cgtggacgac tcggtcgttc accgacgagc agatgctcgc
     2701 gggaaagccg tgatagccca ggaaggacgg ggtgccgcag gcatcgcgga tcaccgattc
     2761 ggcgacgttg tcgagttcga gcgtggatac cccgggcgcg gcggccttgc gcaccgcatg
     2821 cagcgcagcg gcgaccagcg cacctgcggc ggccatcgcg tcgagttcac ctggcgtgcg
     2881 cggatcgacg accttgcggg tgcgcagccc cgggacggtg atcatcggct acttgccgag
     2941 tgagcggagc gcgcgggcga agacctcgtc gagaccgccg accgcgtcga cggtctgcag
     3001 gttgttgtgg ctgtagtact cgagcagggg cctggtctcg tcggagtaaa ccttcatccg
     3061 gttgtggatg acgtcttcgg tgtcatcggc gcggccgcgg gccttgaggc gcgagaacag
     3121 ctcctcttcg gagactgcga attcgagcac cgcgtcgagc ttggtgttgt ggttcttgag
     3181 catctcatcg agcgcctcgg cctgcgccac cgatcgcggg tagccgtcca ggatgaaccc
     3241 gtcgacggca tccgactgct cgatgcggtc ctcgaccaat ttgttcgtca gctccgacgg
     3301 caccaggtcg cctgcgtcca ggtaacgctt ggcctcaagg cccaggggtg tgccggagct
     3361 gatgttctcg cggaagaggt ccccggtgga gatctgcgga atgccgagct tctcggagag
     3421 cttctgggcc tgtgtcccct tgcccgcccc cggcgggccc agcagaacga ctctcacttc
     3481 aggaaccctt cgtagttgcg ctgcatcagc tggctctcga tctgtttgac cgtatccaaa
     3541 ccgacgccaa ccatgatgag aaccgctgtc ccgccgaacg gcaagttctg cacacctccg
     3601 gagttgccga tctcgaggaa caggttcggc aggacggcga tgacgccgag gtagatcgag
     3661 ccgggaaggg tgatcctgtt gagcacgaag cgcaggtagt cggcggtcgg cttgcccgga
     3721 cggataccgg ggatgaagcc gccgtacttc ttcatctcgt cggcgcgctc gtcgacgttg
     3781 aactgcaccg acacgtagaa gtaggtgaag aagacgatca ggccgaagta gatcgcgatg
     3841 taggaggggc tggccgggtt ggtcagatag ttcgcgacga gcttgtccca ccagccgttc
     3901 cctggggtgg cctgcccgct ctggatgagc tgggtgatca ggtgcgggat atagatcagc
     3961 gacgatgcga agatcaccgg gatgacaccg gcctggttga ccttcagtgg cagataggtc
     4021 gacgtgccgc cgtacatctt gcggccgacc attcgcttgg cgtactgcac cgggatgcgg
     4081 cgctgaccct gctcgacgaa gaccacgccg acgatgatga ccagtgcggc gaagcagact
     4141 gcggtgaaga cgaccggccc gcgactgtcg aggatgctct tgccctcgga cgggatgcgg
     4201 gcggcgatgc tggcgaagat catcagcgac atgccgttgc cgatgccacg ttcctgaatc
     4261 agctcgccca tccacatcac cagcgtcgcg cccgcggtca tcacgagcac gatgacggcc
     4321 agcgagaaga tcgacgtgct ctggatgatg tcgaggctgc agccctgcag cagcccgcca
     4381 ttggcggcca gcgccacgat gctggtggcc tgcaggagcg ccagcgcgac cgacaggtag
     4441 cgggtgtact gggtgagttt ggcctgaccc gcttgcccct ctttgcgcag ctcttcgaag
     4501 cgcgggatga ccaccccgag cagctgaacg atgatgctcg cggtgatgta aggcatgatg
     4561 ccgatcgcga acactgccag cttcagcaga gcgccgccgg aaaacaggtt gatcagcgag
     4621 taaatctgcg ccgagtcgcc accgctgagc tgtgtgaggc acttctcgac gttgccgtag
     4681 ttgacacccg gcgacgggat cgacgcgccg acccggtaca ggatcaggat ggccagagtg
     4741 aagagaatct tgcgccgtag atcagccgtc cgcaacgatg agatgaaagc cgagagcact
     4801 cttcctcctg cgcagccggg ctcatatcgc ggcgtgccaa atggggctgg tctctaccag
     4861 cgtcggttaa gtcgtgtgcg agtgccccgg gcagcctggg tgcttgtcgg cccaggccat
     4921 ctgacctgct cccagcctgc ggagtcacgc agtctacgag agtaacagtt ggtggttgtc
     4981 tactccgcat ccgctggtgc aggcactgag gtcagacgac ttttcaggat aaaagcgtag
     5041 agtcggtgac agctcgttac atcagctaag taattgacgg atagcctaac gacaggggtt
     5101 ttcttcatgg cacggacaga caacgacacc tgggatctgg cctccagtgt gggagcgacc
     5161 gcgacgatgg tggcggccgc tcgcgcgatg gggaccagga tcgacggcgc gttgatcagc
     5221 gacccctacg ccgaacccct ggtccgggcc gtcggcgtcg acgtgttcac ccggctggcc
     5281 actggcgagc tggacccctc ggtgttcaac atcgatgacg acacgaacgc cgggatggac
     5341 cgcatggtcg acaacatggc ggtccggacg aagttcttcg acgacttctt caccgacgcg
     5401 acggcggcgg gcgtccggca ggtggtgatc ctcgcgtccg gcctggattc gcgcgcgtac
     5461 cggctgcagt ggcccgccgg caccgtcgtc tacgaggtgg accagcccga ggtgatcgcg
     5521 ttcaagacgc gggcgctcgc cgagatcggc gccgagccga cctgcgaccg ccgggtggtg
     5581 gcgatcgatc tgcgcttcga ctggcctgcc gcgctcaagg aggccgggtt cgaccccgac
     5641 cagccgacgg cgtggagcgc tgaaggactc ctcggctatc tgcctgccga ggcccaggac
     5701 cggctgctgg acaccatgac cgagttgagt gcgccgggaa gctggttggc caccgaaagc
     5761 acaccaccca tcaatcagga cgacgccgac gaggcgatga agaagatgca gacggcagcc
     5821 gagcggtggc gggagcacgg ttacgacctc gactgggcca acctcgtcta cctcggcgac
     5881 cgcaacgagc cggccgccta tctcaccgag cgcggctgga acaccgacgg cagcaacgtc
     5941 aatgagctgc tgaccgccaa cggctttccg ccgttcgacg gcgacacgcc gttcgccgat
     6001 ttgcgttaca tcagcggcac cttcggcaat cactgaa
//
LOCUS       DASYLX010000327         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3744898,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000327 DASYLX010000000
VERSION     DASYLX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744898"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VGP27_07955"
     CDS             complement(<1..775)
                     /locus_tag="VGP27_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7851284.1"
                     /translation="MTVGALFPSPAEVEANTPASRDRAIDVIRIVSLVGVVVGHTIMA
                     TSIISDDVLFWSNLLTASVVFQALTWVFQIMPLFFFAGVAACVDPPPQAGGPPAWEGN
                     APRHTGDSWGNWLMRRCTRLYRPVFYYLAFWISALAILRYFLPEHVYEPVAGISIQLL
                     WFLGAYVLVLAAVPLLTRITTTARLLGAVAGTYALIAVVDAVRINVDGLPAVGYLNLV
                     AWLIPGMLGVAYRRHLLAGRAALMLAAVMFVVNLSLMWVG"
     gene            complement(878..2071)
                     /locus_tag="VGP27_07960"
     CDS             complement(878..2071)
                     /locus_tag="VGP27_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851285.1"
                     /translation="MLETSPRIRYAFVLLAYAFAAIMVGTTLPTPMYALYADRMHFGV
                     LTTTVIYATYAFGVLFALLAFGRWSDAIGRRPVLLAGTFFALASAAVFLAADSVPLLL
                     VGRVLSGLSAGVFTGTATAAVIEAAPPSWRNRAAAVATAANVGGLGGGPLLAGLLVQY
                     APQPLRLTFIVHIGLVVLAAVAVLVVPETSERTGSLGLQRLAVPPEVRATFTLAAIAA
                     FAGFAVTGLFASVAPAFLSGVVGITNHAVAGAVASAVFIASAVTQLFAGRIPPKRALV
                     LGCAILVVAMVILSVALQLSSLWGLIAAAVVGGVGQGVCFSRGLAAVSELTPPGRRAE
                     ISSAYFVVAYVAISLPVIGEGLAARVWGLRTAGVSFAVAVAVLAIVCLVAIMVRERRL
                     TTTEI"
     gene            complement(2114..>2810)
                     /locus_tag="VGP27_07965"
     CDS             complement(2114..>2810)
                     /locus_tag="VGP27_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEV7851286.1"
                     /translation="ALVGPDAILAAPGAQNSAAPLLAFELGGVVLLGIISAVAFATIL
                     AVVAGLTITAATSFAHDIYASVIRGTRGKRPASESEQVRVSRITVVVIGVLAILLGIL
                     AAEQNIAFLVALAFAVAAAANLPTILYSLYWQRFNTRGALWSMYGGLISTVVLIIFSP
                     AVSGAKTAMLPNVDFAWFPLANPGIVSIPLAFLLGIVGTLTSSDRGDPELNAEFEVRT
                     LTGVGAEKAVTAH"
BASE COUNT          572 a          933 c          940 g          365 t
ORIGIN      
        1 gccccaccca catcagcgac aggttgacga cgaacatgac cgcggcgagc atcagggccg
       61 cgcggcctgc gagaagatgc cgacggtagg cgacgccgag catgccgggg atcaaccagg
      121 cgaccaggtt gagatagccc acggcgggca gcccgtcgac gttgatgcga accgcgtcga
      181 cgaccgcaat gagcgcatac gttccggcga cggcgcccaa tagacgggcc gtggtggtga
      241 tgcgggtcag cagcggaaca gccgccagca caagcacata cgcgccgagg aaccacagca
      301 gctggattga gatcccggcg accggttcgt agacgtgctc cggcaggaag tagcgcagta
      361 tcgccagcgc agagatccag aacgccaggt agtagaacac cggccggtac agccgggtgc
      421 agcggcgcat cagccagttg ccccagctgt caccggtgtg cctgggggca ttcccctccc
      481 aagctggggg gccacccgct tgcgggggag ggtcgacgca cgccgcgaca cccgcgaaga
      541 agaacagcgg catgatctgg aacacccagg tcagcgcctg gaagacgacg gaggcggtga
      601 gcaggttgct ccagaaaagc acgtcgtcgc tgatgatgct cgtcgccatg atcgtgtgtc
      661 cgacgaccac gccgaccagt gaaacgatcc ggatgacgtc gatggcacgg tcccgcgatg
      721 cgggcgtgtt cgcctcgacc tcagccgggc tgggaaagag cgcgccaacg gtcatgcctc
      781 ggcgcccagt cgctgcagtc ctcactcgtg gtcgcctgcg ggcgggcatg ccgcaaatct
      841 aggcccgacc tgcgcgtgag tgtgtgagta gaagcactca gatttcggtc gtggtcagcc
      901 tgcgttcgcg gaccatgatc gccacgaggc agacgatcgc cagtaccgcg acggcaaccg
      961 cgaagctgac cccggcggtg cgcagacccc acacccgcgc cgcaagacct tcgccgatta
     1021 ccggcagcga gatcgccacg taagccacga cgaagtacgc ggagctgatt tcggcccgtc
     1081 ggcccggcgg ggtgagctcg gagacggccg ccaggccgcg gctgaagcag acgccttggc
     1141 cgacaccgcc gacgactgcg gcagcgatca gcccccacag tgacgaaagc tgcagtgcga
     1201 cgctgagtat caccatggcg acaacgagga tcgcgcagcc gagaaccaac gcgcgcttcg
     1261 gtgggatccg gcccgcgaac aactgggtca ccgccgaggc gatgaacacg gcggacgcga
     1321 cggcgccggc cacggcatgg ttggtgatgc cgacgacgcc ggacaaaaac gccggtgcaa
     1381 ccgaggcgaa cagtcccgtt acggcgaagc ccgcgaatgc cgcgatggcc gcaagcgtga
     1441 acgtggcgcg aacctcgggc ggtaccgcaa gccgttgcaa cccaaggctg cccgtgcgct
     1501 ccgatgtttc cggtacgacg aggaccgcca ccgcggccag tacaaccagc ccgatgtgca
     1561 cgatgaatgt cagacgaagc ggctgaggcg catattggac cagcagccct gccagcagcg
     1621 ggccaccgcc gagaccgccg acgttggcgg ccgtcgccac cgcggcagcg cggttgcgcc
     1681 agctcggtgg cgccgcttcg atgacggcag cggtggccgt gccggtgaag acgcctgccg
     1741 acaaccccga cagcactcgg ccgaccagca gcagcggcac ggaatcggcg gccaaaaaca
     1801 ctgcggcgct ggcgagtgcg aagaaggtgc cggccagcag caccggtcgg cggccgatgg
     1861 cgtccgacca ccgcccgaag gccagcagtg cgaacagcac gccgaacgca tacgtcgcgt
     1921 agatgaccgt ggtggtcagc acgccgaaat gcatgcgatc ggcgtacagc gcgtacatcg
     1981 gcgtcggcag tgtggtgccg accatgatcg ccgcaaaagc gtatgcaagc aggacgaacg
     2041 catagcgaat cctgggcgat gtttcgagca tgtgggactt cagcggttcg gccacctgct
     2101 ctattccacc agatcagtgt gctgtgaccg ccttctcggc gccgacgccc gtcagcgttc
     2161 gcacctcgaa ctccgcgttc aactccgggt cgccgcgatc cgacgacgtc agcgtgccca
     2221 cgatgcccag caggaaagcc agcggaatcg acacgatgcc cggattggcc agcgggaacc
     2281 acgcgaagtc cacgttcggc aacatcgcgg tcttggcacc cgaaaccgcg ggcgagaaga
     2341 tgatcaacac gaccgtcgaa atcaacccgc cgtacatgct ccacagcgca ccgcgggtgt
     2401 tgaaccgttg ccagtacagc gaatacagga tcgtcggcag gttggctgct gccgccaccg
     2461 cgaaagccag cgctaccagg aacgcgatgt tctgttcggc ggcgaggatg cccagcagga
     2521 tggcgaggac gccgatcacc acgacggtga tgcgcgacac ccgtacctgt tcgctctcgc
     2581 tcgctgggcg cttcccgcgg gttccacgga taacgctggc gtagatgtcg tgcgcaaacg
     2641 acgtcgcagc ggtgatggtc aggccggcaa ccacggcgag gatcgtcgcg aacgccaccg
     2701 ccgagatgat gccgagcagg acgacaccgc ccagctcgaa cgccagcagc ggcgccgccg
     2761 aattctgtgc gccgggagcg gcgagtatcg cgtcgggtcc taccagtgcc
//
LOCUS       DASYLX010000328         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1440250,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000328 DASYLX010000000
VERSION     DASYLX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440250"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..795
                     /locus_tag="VGP27_07970"
     CDS             190..795
                     /locus_tag="VGP27_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851287.1"
                     /translation="MKQRSKGERTRAAILMEAARLATVDGLDGLSIGGLATALGMSKS
                     GLYAHFGSKEDLQLATVASARETFVAEVFVPALDAPQGIRRLYAACDAFLSHIERRVF
                     PGGCFFAVAAADVGTRQGPVRDAVAAQQQDWSRLLERLARKAMEVGQLEAGVDPAQLA
                     FELNAILASASTTFVLQGDAAVLERARAAVRRRLPTAGKFS"
     gene            complement(788..1366)
                     /locus_tag="VGP27_07975"
     CDS             complement(788..1366)
                     /locus_tag="VGP27_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851288.1"
                     /translation="MNRLAAAGAAAALTAVLTHPATATAASNTATTAIPVDPATNIEM
                     HVTANCVSAENRCYFDTTANLTTPDGPTGFPGDTWARQTITIRSSSRDVWQEASYSAP
                     SGNPREVKGANHENVLSKMYKAINNVEISITYFGGGPIERFKADGDSVPTEWATGRPN
                     TQAAFFACSQIQVVYGGVNLTTPTACAQTTFN"
     gene            complement(1406..2482)
                     /locus_tag="VGP27_07980"
     CDS             complement(1406..2482)
                     /locus_tag="VGP27_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEV7851289.1"
                     /translation="MTDEPLGSHVLELQVAEVVVETDDARSLVFCVPDGPDAPEIPAD
                     RLRYQPGQFLTLRVPSDRTGCVARCYSLCSSPHTGDPLTVTVKRTDGGYASHWLCDNA
                     HPGMRIHALAPSGNFVPKTLDTDFLLLAAGSGITPMMAICKSALAEGNGRVVLIYANR
                     DENSVIFGVALRELAAKYPDRLTVVHWLESVQGLPSAAALSALAAPYAGHDAYICGPG
                     PFMAAAEEAMKNAGAAADRIHIEVFKSLESDPFARVVIAAEDSDDSDEGPATAIVTLD
                     GDRHEIRWPRSAKLLDVLLDKGLDAPFSCREGHCGACAVLKKSGDVEMEVNDVLEQQD
                     LDEGLILGCQARPRSDSVEVTYDE"
     gene            2697..>3109
                     /locus_tag="VGP27_07985"
     CDS             2697..>3109
                     /locus_tag="VGP27_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851290.1"
                     /translation="MTSIEQRDVQSVLAGIDDLLPLIAKRAQATEDLRRLPDETVAEL
                     DEVGFFKLLQPEQWGGLQCDPTAFYEAVRRIATACGSTGWVSSIVGVHNWHLALFDQQ
                     AQEEVWGADPTVRISSSYAPMGAGHVVDGGYLVSGS"
BASE COUNT          499 a          992 c         1051 g          567 t
ORIGIN      
        1 cacgacttcc tcaacacgcg ctgcctgacc atcgcgggcg gcaccgagca gatcctgctc
       61 acgatggcag gcgaacggct gctcggcctg ccccgctagc aggtcctcac ctcgccgtag
      121 tggcgaaaag cacgagcgtg cttttagcga atacggtaat agcacggtcg ttcctttatg
      181 ctcaatgccg tgaaacagcg gtcgaagggc gagcgcacca gggccgcgat cctcatggag
      241 gccgcgcgtc tggcgacagt cgatggactg gacggtctct ccatcggcgg gctggccaca
      301 gcgctcggga tgagcaagag cggtctttac gctcacttcg gctcgaaaga ggacctgcag
      361 ctggcgaccg tcgcgtctgc gcgggagacc ttcgtcgccg aggtgtttgt gcctgccctt
      421 gacgcaccac agggcatccg gcgcctgtac gccgcatgcg acgcgttcct gtctcacatc
      481 gagcggcgcg tgttccccgg cggatgcttc ttcgcagtag cggccgccga cgtcggaacc
      541 cgtcagggtc cggtgcgtga cgcggtcgct gcgcagcaac aggactggag ccggctgttg
      601 gagcggcttg cccgcaaggc gatggaagtc gggcaactgg aggcaggcgt ggatccggcc
      661 cagctggctt tcgagctcaa cgcgattctg gcctccgcca gtacgacttt cgttctccaa
      721 ggagacgcag cggtgttgga gcgcgctcgt gcggctgtcc gccgacggtt gccgacggcg
      781 gggaagttca gttgaacgtg gtctgcgcgc aggccgtcgg cgtagtcagg ttcacccctc
      841 cgtagaccac ctggatctgc gagcacgcga agaaggcggc ctgggtgttc ggacgcccgg
      901 tggcccactc ggtgggcacc gaatcgccgt cggccttgaa gcgctcgatc ggtccaccgc
      961 cgaagtaggt gatcgagatc tcgacgttgt tgatcgcttt gtacatcttc gataacacgt
     1021 tctcgtggtt ggcgcccttg acctcgcggg gattgcccga gggggcgctg taggacgctt
     1081 cctgccacac gtcgcggctg gaactacgaa tcgtgatggt ctgccttgcc cacgtgtcac
     1141 cggggaatcc ggtcgggccg tcgggtgtcg tcaggttggc ggtggtgtcg aaatagcatc
     1201 tgttctccgc cgacacgcag ttggcggtga cgtgcatttc gatgttggtc gccggatcga
     1261 ccgggatcgc ggtggtggcg gtgttggacg cggccgtcgc cgttgcggga tgcgtgagca
     1321 cagcggtcag cgctgccgcc gcgcctgctg ctgcaagccg attcatttgt gctcccctcc
     1381 agtgcggcga atggtagccc ggcgcttatt cgtcgtaggt gacttccacg gaatccgagc
     1441 gcggtcgcgc ctggcagccg aggatcaggc cctcgtcgag atcctgctgc tcgagcacgt
     1501 cgttgacctc catctcgaca tcgccgctct tcttcagaac ggcgcaggcg ccgcagtggc
     1561 cctcgcggca ggagaacggt gcgtcgagac ccttgtcgag cagcacgtcg agcagtttcg
     1621 cgctgcgggg ccagcgtatt tcgtgcctgt cgccgtcgag cgtgacgatc gctgtcgcag
     1681 gcccctcgtc gctgtcgtct gaatcttcgg ccgcaatcac caccctggcg aacggatccg
     1741 attccagtga cttgaacacc tcgatgtgga tccggtcggc agcggcgcct gcgttcttca
     1801 tcgcctcttc ggccgccgcc atgaacggcc cgggcccgca gatgtatgcg tcgtggccgg
     1861 cgtacggagc ggcgagagcg gacagcgcgg ccgcactcgg cagtccctgc acggattcca
     1921 gccagtgcac gaccgtcagc cggtcggggt acttcgcggc gagttcccgc agcgcgacac
     1981 cgaagatgac ggagttctcg tcgcggttgg cgtagatcag cacgacgcgg ccattgccct
     2041 cggcaagggc cgacttgcag atcgccatca tcggcgtgat gccgctgccc gcggccagca
     2101 gcaggaaatc ggtgtccagc gtcttgggca cgaagtttcc cgacggcgcc agcgcatgga
     2161 tgcgcatgcc ggggtgggcg ttgtcgcaca gccagtgcga ggcgtatccg ccgtcggtcc
     2221 gtttgacggt gaccgtcaac gggtcgccgg tgtgcgggga gctgcacagc gagtagcagc
     2281 gggcgacaca gccggtgcgg tcgctgggca cccgcagcgt gaggaactgt cccggctgat
     2341 aacgcagccg gtcggcggga atctcgggag cgtcgggacc atcgggaaca cagaacacca
     2401 gcgagcgcgc gtcgtcggtc tcgacgacga cctcggccac ctgcagttcc agcacgtggc
     2461 taccgagcgg ctcatccgtc acgcggttgc ccttcctact ccggcccggg ggccataact
     2521 agaacaggtt acagaaatgc atgtgcgcag gtccagccgc cacaggaatg cgctgctcga
     2581 cacaaatcgt aacgtgttct aatcttttcc caagcgatct gtcccctacc cttcgggtgg
     2641 ggccccactt cgcggaagcg ctcatcgcca ggtcctttag ctcgggaggc aaatcagtga
     2701 cgtccattga acagcgtgac gtgcagtcgg tcctagccgg catcgacgat ctgctgccgc
     2761 tgatcgccaa gcgagcgcag gcgacggaag accttcgacg gctgccggac gagacggtcg
     2821 cagagctaga cgaggtcggc ttcttcaaac tgctccagcc cgagcagtgg ggcggccttc
     2881 agtgcgatcc gacggcgttc tacgaagcgg tgcgtcgcat cgccacggcg tgcggatcga
     2941 ccgggtgggt cagctcgatc gtcggcgtgc acaactggca cctggccctg ttcgaccagc
     3001 aggcgcagga agaggtgtgg ggcgccgacc cgaccgtgcg catctcctcg tcgtacgcac
     3061 cgatgggtgc gggacacgtg gtcgacggcg gctacctcgt cagcggctc
//
LOCUS       DASYLX010000329         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3415771,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000329 DASYLX010000000
VERSION     DASYLX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415771"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="phoU"
                     /locus_tag="VGP27_07990"
     CDS             <1..591
                     /gene="phoU"
                     /locus_tag="VGP27_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV7851291.1"
                     /translation="AMERATQALLQADLVLAEQVITDHEQIAAMSAKAEEAAFVLLAL
                     QAPVAGDLRAIVSSIQIVADVDRMGALALHVAKIARRRHPQHALPEEVNGYFAEMGRV
                     AVELGNSAQEVLLTKDPEKAARISEEDDAMDDLHRHLFSVLMDKEWRHGVTAAVDVTL
                     LSRFYERFADHAVEVARRIIFSATGRFPEDAELPAR"
     gene            complement(598..1524)
                     /locus_tag="VGP27_07995"
     CDS             complement(598..1524)
                     /locus_tag="VGP27_07995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV7851292.1"
                     /translation="MSKAVRFDRYGDIEVLEVRDVDRPAPAAGEVLVEVRAAGINPGE
                     ASIRKGLLSDVFPTTFPSGQGSDLAGVVAETGPGVDGFAVGDEVLGFSEKRASHAEFI
                     VVPAENLTAKPAAVSWEVAGALYVAGTSAYAAVRAVGLSPGDVVVVAGAAGGVGSIAV
                     QLAVEAGATVLGIAGPSNDEWLRGHGVIPVNYGDDLAERLRAAAPTGRVNAFLDFFGG
                     GYVELAVDQLGVAPDRVDTIIDFAAAEQFKVRTAGNADAANADVLAELAALVADGKLE
                     VPVAEVFALDDVQKAFRSLEQRHTRGKIVLRP"
     gene            complement(1581..2357)
                     /gene="pstB"
                     /locus_tag="VGP27_08000"
     CDS             complement(1581..2357)
                     /gene="pstB"
                     /locus_tag="VGP27_08000"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV7851293.1"
                     /translation="MAKRLDLKDVNIYYGSFHAVADVSMSVPPRSVTAFIGPSGCGKS
                     TVLRTLNRMHEVIPGARVEGSVLLDGEDVYGAGVDPVGVRKTIGMVFQRPNPFPTMSI
                     RDNVVAGLRLQGVRNKKTLDEVAEKSLKGANLWTEVKDRMDRPGGGLSGGQQQRLCIA
                     RAIAVQPDVLLMDEPCSALDPISTLAIEDLIAELKQDYTIVIVTHNMQQAARVSDQTA
                     FFNLEATGRPGKLIEIDDTEKIFSNPTEKATEDYISGRFG"
     gene            complement(2369..3283)
                     /gene="pstA"
                     /locus_tag="VGP27_08005"
     CDS             complement(2369..3283)
                     /gene="pstA"
                     /locus_tag="VGP27_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV7851294.1"
                     /translation="MTATLDQPVKAPTFQKLSLRRKLADNVATVLVTLSVAVAMVPLV
                     WVLYTVIVKGFGAVTSSTWWFNSQAGMTAFQAGGGAYHAIVGTLLQGLVCALISIPIG
                     VFVGIYLVEYGGGTRMGKVTTFMVDILTGVPSIVAALFIYALWVATLGFHRSGFAVSL
                     ALVLLMIPVIVRSTEEMLRIVPMDLREASYALGVPKWKTISAIVIPTALSGIVTGILL
                     ALARVMGETAPLLILVGYAQAINFDMFGGFMGSLPGMMYDQTSAGAGANPVPTDRLWG
                     AAFTLIVLIAVLNVGARFIAKFFAPKKV"
     gene            complement(3280..>3505)
                     /locus_tag="VGP27_08010"
     CDS             complement(3280..>3505)
                     /locus_tag="VGP27_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV7851295.1"
                     /translation="TIALLIILRGTQQAFGWSLFDAGYTFASKIAAAASEFNDQYKAG
                     AYIAAGLVLFILTFIVNSLARAAVAGRSKS"
BASE COUNT          639 a         1213 c         1082 g          571 t
ORIGIN      
        1 gcgatggagc gagcgaccca ggccctgctg caggccgacc tcgtgctggc cgaacaggtg
       61 atcaccgacc acgagcagat tgccgcgatg agcgccaagg cggaggaagc ggcctttgtg
      121 ctgcttgccc tgcaggctcc cgtcgcgggc gatctacgcg cgatcgtcag ctcgatccag
      181 atcgtcgccg acgtcgatcg aatgggggcc ctggcgctac acgtagccaa gatcgcccgc
      241 cgccgtcatc cccagcatgc cctgcccgaa gaggtcaacg gctacttcgc cgaaatgggc
      301 cgggtggcag tcgaactcgg caacagcgcc caagaggtgc tgctgaccaa ggacccggag
      361 aaggcggcgc gcatcagcga ggaagacgat gcgatggacg atctgcaccg tcatctgttc
      421 tccgtgctga tggacaagga atggaggcac ggcgtcaccg ccgccgtcga cgtgacgttg
      481 ctcagccgct tctacgagcg cttcgccgac cacgccgtcg aggttgcccg ccggatcatc
      541 ttctcggcga cgggcaggtt ccccgaggat gcggaacttc ccgcccgcta gccgccgcta
      601 cgggcgaagc acgatcttcc ctctggtatg ccgctgctcg agggagcgga acgccttttg
      661 gacgtcgtcg agagcgaata cctcggcgac cggcacctcc agctttccgt cggcgaccag
      721 cgccgccagt tctgcgagca cgtcggcatt ggcggcgtcc gcgttgccgg cggtgcgcac
      781 cttgaactgc tcggcggcgg cgaaatcgat gatggtgtcc acccggtcgg gcgcgacgcc
      841 cagctgatcg accgccagct cgacgtaccc gccaccgaag aagtccagga aggcgttaac
      901 ccgccccgtt ggcgccgccg cacgcagacg ctcggctaaa tcgtcgccgt agttcacggg
      961 gatcacaccg tgaccgcgca gccactcgtc gttcgacgga cccgcgatgc ccagcaccgt
     1021 ggcgccggct tcgacggcaa gctgcaccgc gatcgaaccc actccccctg ccgcgcctgc
     1081 gacgaccacc acgtcgcccg gcgagaggcc gacggcgcgg acggcggcgt aggcactcgt
     1141 gcctgccacg tacaacgcgc cggccacctc ccacgacacc gccgcgggtt tggcggtcag
     1201 attctcggcg ggcacgacga tgaactcagc gtggctggcc cgcttctcgg aaaagcccag
     1261 aacctcgtcg cccaccgcga atccgtcaac gcccggacct gtttcggcga ccacgcccgc
     1321 caggtcgctg ccctggccag acggaaatgt cgtcgggaag acgtcgctca gcagtccctt
     1381 gcggatgctg gcctcgcccg ggttgatgcc tgccgccctg acttcgacga gaacctcgcc
     1441 tgcggcgggc gcaggccggt cgacgtcgcg cacctcgagc acctcgatgt cgccgtaccg
     1501 atcgaatcgc actgctttgc tcatgtcagt taggaacggc gcagccctcg agctattccc
     1561 gccggtggtg ctgactgcct ctagccgaac cggcccgaga tgtagtcctc cgtcgccttt
     1621 tccgtcgggt tcgagaagat tttctcggtg tcgtcgatct cgatcagctt gccgggcctg
     1681 cccgtcgcct cgaggttgaa gaacgcggtc tgatcgctca cgcgcgcagc ctgctgcatg
     1741 ttgtgcgtga cgatcacgat cgtgtagtcc tgcttgagct cggcgatgag atcctcgatg
     1801 gccagcgtcg agatcgggtc gagggccgag cacggctcgt ccatcaacaa cacgtcgggc
     1861 tgcaccgcga tcgcacgcgc gatgcacaac cgctgctgct ggccaccgga caacccgccg
     1921 ccgggtctgt ccattcggtc cttgacctcg gtccacaggt tcgcgccctt gagggacttc
     1981 tccgccacct cgtcgagagt cttcttgttg cggacgccct gcagcctgag acctgccacc
     2041 acgttgtcgc gaatcgacat ggtggggaag ggattcggcc gctgaaacac cataccgatg
     2101 gtcttgcgga cgccgaccgg gtcgacgcct gcgccataga cgtcctcgcc gtcgagaagc
     2161 accgagccct ccacacgcgc accggggatc acctcgtgca tccggttcaa cgtgcgcagc
     2221 acggtcgact tgccgcagcc ggacggaccg atgaacgccg tcacgctgcg cggcggcacc
     2281 gacatcgaca cgtcggccac ggcgtgaaac gacccgtagt agatgttgac gtccttgagg
     2341 tcgaggcgct tggccatctg agtgactcct aaaccttctt gggggcgaag aacttggcga
     2401 tgaaccgcgc tcctacgttg agcaccgcga tcagcacgat gagagtgaag gcggcacccc
     2461 aaagccggtc cgtcggcacg ggattggcac ctgcacctgc cgaggtctgg tcgtacatca
     2521 tgcccggcag cgatcccatg aacccgccga acatgtcgaa gttgatcgcc tgggcgtagc
     2581 cgaccaggat cagcagtggc gccgtctccc ccatcacccg agccagggcc agcagaatgc
     2641 ccgtcacgat gcccgacagc gctgtcggaa tcacgatggc cgatatcgtc ttccacttcg
     2701 gcacgccgag tgcgtaactc gcttctcgta ggtccatcgg gacgatccgc aacatctcct
     2761 cggtggaccg cacgatcacc gggatcatca acaacaccaa ggcgagcgac acggcgaagc
     2821 ccgaacggtg gaagcccagc gtcgccaccc acagcgcgta gatgaacaag gccgccacga
     2881 tggacggaac accggtgagg atgtccacca tgaaggtggt caccttgccc atccgcgttc
     2941 cgccgccgta ctccaccaga tagatgccga cgaatacgcc gatcgggatc gagatgagcg
     3001 cacacaccaa gccctgcaac agggttccga cgatcgcgtg atacgcgccg ccaccggcct
     3061 ggaacgcggt cattccggcc tgcgagttga accaccacgt gctcgacgtg acggcgccga
     3121 aacctttgac gatcactgtg tagagcaccc acaccagcgg caccatcgcg accgccaccg
     3181 aaagcgtcac caacaccgtg gcgacgttgt cggccagctt gcggcgcagg ctcagcttct
     3241 ggaacgtcgg cgccttgacc ggctgatcca gtgtggccgt catgacttac tccttccggc
     3301 gacggcggcc cgtgccagcg agttgacgat gaacgtcagg atgaagagga cgaggcctgc
     3361 cgcaatgtac gcgcccgcct tgtactggtc gttgaattcc gaagcggcag cggcgatttt
     3421 gctcgcgaac gtatacccgg cgtcgaacag cgaccagccg aacgcctgct gcgtaccgcg
     3481 caggatgatg agcaacgcga tcgtc
//
LOCUS       DASYLX010000330         9274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2646510,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000330 DASYLX010000000
VERSION     DASYLX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646510"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..861
                     /locus_tag="VGP27_08015"
     CDS             400..861
                     /locus_tag="VGP27_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7851296.1"
                     /translation="MVEPKSRAGRRVISLPPPIIPALLEHRKAQENERELAAYLWQGD
                     SWVFAQPIGKPTDPRADYGEWKELLTAAGVREARLHDARHTAATMLLVLGVAERAVMD
                     VMGWSKIDMAQRYMHVPDQLRQQIASQLGGLLWKAPTEDEDDGTAGAPVPA"
     gene            complement(930..1002)
                     /locus_tag="VGP27_08020"
     tRNA            complement(930..1002)
                     /locus_tag="VGP27_08020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Arg,seq:acg)
     gene            complement(1042..1131)
                     /locus_tag="VGP27_08025"
     tRNA            complement(1042..1131)
                     /locus_tag="VGP27_08025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1093..1095),aa:Ser,seq:gct)
     gene            1325..1723
                     /locus_tag="VGP27_08030"
     CDS             1325..1723
                     /locus_tag="VGP27_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851297.1"
                     /translation="MTDTPAPTIWPALTYADAPAAIRFLVEAFGFVEILVVPGEGGRE
                     IAHAELRWPEGGGVMLGSTSDDSVFGQLKPGTTSIYVVTDAPDKVFQRATMAGAEVVR
                     GLADEDYGSRGFTVRDPEGTLWSFGTYRGA"
     gene            complement(1738..3063)
                     /gene="murA"
                     /locus_tag="VGP27_08035"
     CDS             complement(1738..3063)
                     /gene="murA"
                     /locus_tag="VGP27_08035"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV7851298.1"
                     /translation="MTDDGDALHLTGGIPLGGTVKIRGSKNAVPKLMVAALLTDEPVL
                     IHNISQIVDTWLVSDLLEMLGCSTAQPADGQLRIEARGIHTAGTRDLEYFHVRSRIPV
                     LTCAPLLHRTGHAELWKPGGCSIGERPVDLHLEVLRAFGAVSQRTDTGFKLTAEAGGL
                     RGAVVELKYPSVGATEQFLLGAVVAAGDSELHNAATEPEIIDLICMLQRMGALITVQP
                     GRVLQVTGVSRLRGVSHTAIPDRLEAASWASLALATDGRITVHGVRQADLGTYLNVYR
                     RAGGEFDFSSDGNEVTFRREKAMPRPLVIETDVHPGFMTDWQSPMVTALTQADGVTVV
                     HETVHENRLAYMKALQALGAQMQVFTECLGRTPCRFGARNHQHSAVVVGPTKLQGTTV
                     HVPDLRAGFSYVLAALAAQGDSTVTGVSLLDRGYESFRDKLLGVGAKLR"
     gene            complement(3188..5152)
                     /locus_tag="VGP27_08040"
     CDS             complement(3188..5152)
                     /locus_tag="VGP27_08040"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEV7851299.1"
                     /translation="MTEPQILWLPDPDAVARSRMAAFRSWLAEQRGVIVADYEALWRW
                     SVADLAGFWGAFADFAGVRFHTAPQRVLTGESMPRTRWFPGATLNYAEHALVPGPGKS
                     DDDPAMIFRREDGVRDARSFGLLRQQVAAVRSALASLGVRRGDRVVALVPNSPDTLVA
                     FLATASLGAIWSSCSPDFGTRAIVDRFTQLTPTVLIAVDGYLYGGKRFDIRSTVGALR
                     DQLPSLRATVLVPYLDSSVTLDATVCWDDLLAAHCGAELAFEAVDFDHPLWVLYSSGT
                     TGLPKGIVHGHGGIILEHLRALALQADLGPGQRFFWFTTTGWMMWNYLISGLLVGATI
                     VLYDGSPAHPDLGSLWRLAADERVNYFGTSAPFVQSCGKAGLRPGAELDLTALRAVGS
                     TGAPLPAEGFRWIASEIGSGVQVCSVSGGTDLCAAFLCAAPDVPVWEGEISCRALGAA
                     VAALDEDGRELIDEVGELVITKPMPSMPVCFWNDPDGTRLREAYFEVYPGLWRHGDWI
                     KITPRGSSVIYGRSDATLNRGGVRMGTAEFYAVVEGADEVLDSLVIDTSGAGADQGEL
                     LCFLVLAAGVSLPDVEPWLRAALRSGLSPRHVPNRFIVIDEVPRTLNGKKCEVPVKNI
                     LAGMDPARALSSGALRNPDALHPFLALRSP"
     gene            complement(5221..5901)
                     /locus_tag="VGP27_08045"
     CDS             complement(5221..5901)
                     /locus_tag="VGP27_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7851300.1"
                     /translation="MTQRSLEQIPLCDGTGLRLTVATPESAVRGGIVVEPDVGGVTDD
                     VWQLAAGLAGEGWLAVIPDLFHCDDIDELPAGGATARRHLERLTETSVHADTDAALRW
                     LARRRVAADQMGVVGFGLGGAAALIVATKRDLGAAVTIGGIGAVEPVAAGLPALVDVA
                     AELRCPWLGIYDQDGDVPEAEVTKLRDAAHSAHVATDLVHCSFHTDQSVASEAWGRTL
                     NWFSSHLR"
     gene            6091..7173
                     /gene="hisC"
                     /locus_tag="VGP27_08050"
     CDS             6091..7173
                     /gene="hisC"
                     /locus_tag="VGP27_08050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV7851301.1"
                     /translation="MTAHTRADLESLPGYVPGRSIPGAVKLASNEVSVGPLPGVVAAI
                     RDAAAGINRYPDNGAAALVDRLAARLDVPPSQLAVGAGSVALCQQLVQATCTPTDEVL
                     FAWRSFEAYPIITQIVGARQRTVPLRTDHTHDLAGMLDAITPATRLVFVCNPNNPTGT
                     AVRRDELVTFLDAVPDGVLVALDEAYHEFVTDPAVPDGMELYRQRENVAVLRTFSKAY
                     GLAALRVGYCVAPEPVATTLRKVSVPFSINSLAQVAALASLDVEDEMLARCRSVAAER
                     DRVGSELIAMGYPVPRSQANFVWLPFGEHTMAFNEHCLEHKVVIRPFSGDGVRITIGD
                     PNENEMALAAAREWQNSDPQGRQPPI"
     gene            complement(7158..8180)
                     /gene="moaA"
                     /locus_tag="VGP27_08055"
     CDS             complement(7158..8180)
                     /gene="moaA"
                     /locus_tag="VGP27_08055"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV7851302.1"
                     /translation="MDGVNTVNDQLGRGLRDLRISITDRCNFRCCYCMPRDVFGTDFP
                     FMDSSELLSFEEIVRVTKVFAGLGVEKLRLTGGEPLLRHGIEGLVELLAGIDGIDDVA
                     MTTNGSLLAKKARSLRDAGLSRVTVSLDSLDAQTFRTMSDVKIPVSRVLEGIAAAADA
                     GLTPVKLNTVVKRGLNDGKGILDLAAFGREHGYIVRFIEFMDVGATNGWRMDDVVAAA
                     EIVEAINGQWPIEPVDPAYVGEVAARYRYADGAGEVGVITSISQPFCGTCTRARLSAK
                     GELYTCLFAGLGHDLRTPLRDGTSDDAIEKRITGIWKDRTDRYSAQRTTATLGLPKVE
                     MSYIGG"
     gene            8419..9030
                     /locus_tag="VGP27_08060"
     CDS             8419..9030
                     /locus_tag="VGP27_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851303.1"
                     /translation="MSAPASPYRFLAPQPRHQRPTKRVGVVSFTLGLLVVFGLVVYAT
                     WLGVAALATVDGATQRVGATGTTTRLATAPPKAAPPAVVVTPGGTTMAFDQLWTASDG
                     NTIVAGAPTVGTSMQTGEPVILVPVTLTNNGERDWNPVYTSFVGKLNHATVPESAEGD
                     WMYRSPIVAHTSVTLTKVFVGGPGQFSLTANTPNGVALFSGRI"
BASE COUNT         1644 a         3112 c         3035 g         1483 t
ORIGIN      
        1 cgcatcacga agaatcctat ccgagtagtc cgatcaccgc acacggagga aaccgagatc
       61 gagccgttga ccgtggacga tgctcacgcg atcctcgcag tggcgcgaga gaggagaaac
      121 ggtgtccggt gggccgtagc gctcgctttg gggctacgtc agggcgaggc gctagggctc
      181 aaatggtcgg acatcacgac cgaatggcac cacggctgtc cggaaacgaa tccgtgtggg
      241 atgcgaccgg agaactgccc gcgacggcac gcgacgggca ccctcacggt tcgccgagcg
      301 atccagcggc agacctggca gcacggctgc ggtccgggcg agatttgcgg ccggaaacgc
      361 ggtgcgaatt gcccacgtcg gcacgggggt ggtctgatcg tggtggagcc gaagtcccgc
      421 gccggtcgtc gggtaatcag cttgccacca ccgataatcc cggccctgct cgaacaccga
      481 aaggcgcagg aaaacgaacg tgaactggcg gcctatctgt ggcagggcga cagctgggtg
      541 ttcgcgcagc cgatcgggaa gccgaccgac ccgcgcgccg actacggcga gtggaaggag
      601 ttgctaacgg cggccggtgt acgcgaggcc cggctacacg atgcaagaca cacggcagcg
      661 accatgctgc ttgtactggg ggtggctgag cgtgccgtga tggatgtcat gggctggtcg
      721 aagatcgaca tggcgcagag gtacatgcac gtacccgatc agctcaggca gcagatcgcc
      781 tcacagctcg gtgggctgct ctggaaggca cccacggagg acgaagacga cggcacggcg
      841 ggcgcccccg ttcccgcctg aaatggtcaa ttgagacccc atttgaaacc cagaaggggt
      901 gtcccgcgcg ggacacccct tctcacctgc gcgcccggag agactcgaac tcccaacctt
      961 ctgatccgta gtcagatgct ctatccgttg agctacgggc gcctgttctg ttgtcggcca
     1021 gcatagggat ctgaccgtcc gcggaggctc cgggatttga acccgggagg gggcgtaacc
     1081 cccaaccgca ttagcagtgc ggcgccatag accagactag gcgaagcctc ccggggccct
     1141 tcgaggccgc cagacgagca gcctacacag cgtgcccttg atcgagcaaa gcgggtaacc
     1201 gaccgcccga tggcgtcaac actgctggtc aaagcggtac caacaggtga acaaagcggc
     1261 tgaggcgtcc gatgacgtcg cgtccacctg ccggtggcac cgcagaccgt gcatcctcgg
     1321 tgacatgacc gacactccag cacccaccat ttggcccgca ctgacctatg ccgacgcgcc
     1381 ggctgccatc cgcttcctgg tcgaagcctt cgggttcgtc gaaatcctcg tcgtgcccgg
     1441 ggaaggcggc cgggagatcg cgcacgccga gctgcgctgg cccgaaggcg gcggcgtcat
     1501 gctcggctcg acctcggacg acagcgtatt cggccagttg aagccaggga cgacatcgat
     1561 atacgtggta accgacgccc ccgacaaggt gttccagcgg gccaccatgg ccggcgccga
     1621 agtcgtccgc ggcctggcgg acgaggacta cggctcacgc gggttcaccg tccgggaccc
     1681 ggaaggcacc ctgtggagtt tcggcaccta ccgcggagcg tgacgccgcg tcgtcagtca
     1741 gcgaagtttg gctccgacgc cgagcagctt gtctcggaag ctctcgtagc cgcggtcgag
     1801 cagtgagact ccggtcaccg tcgagtcgcc ctgcgccgcg agtgcggcca gcacgtagga
     1861 gaaacccgcg cgcaggtccg gaacgtgcac ggtcgtgcct tgcagcttgg tcggtccgac
     1921 cacgacggcg gagtgttggt gattgcgcgc tccgaaccgg cacggggtac gcccgaggca
     1981 ctcggtgaag acctgcatct gagcgcccag cgcctgaagt gccttcatgt acgccagccg
     2041 gttttcgtgc accgtctcgt gcaccaccgt cacaccgtcg gcctgggtca gtgcggtcac
     2101 catgggggac tgccagtcag tcatgaagcc cgggtgcacg tcagtctcga tgaccagtgg
     2161 gcggggcatg gccttctccc gccggaacgt cacttcgttg ccgtcgctgg agaagtcgaa
     2221 ctcgccgccc gcgcgccggt agacgttgag atacgtccca agatcggctt gccggacgcc
     2281 gtgcaccgtg atccgtccgt cggtagccaa agcgaggctc gcccacgaag ccgcttcgag
     2341 ccgatccggg atggcagtgt gcgagacacc gcgcagccgg gagacgccgg tgacctgcag
     2401 gacacggccg ggctggacgg tgatcagtgc acccatccgc tgcagcatgc agatcaggtc
     2461 gatgatctcc ggctcggtgg cggcgttgtg cagctcggag tcgcccgcgg cgacgactgc
     2521 gcccagcagg aactgctcgg tcgcgcccac gctcgggtac ttcaactcga cgacggcgcc
     2581 gcgcaaccca cccgcctcgg ccgtcagctt gaagccggtg tcggtgcgct ggctgaccgc
     2641 cccgaaggcg cgaaggacct ccaggtgcag atcgaccgga cgctcgccga tgctgcaacc
     2701 accgggcttc cacagctccg cgtgaccggt gcggtgcagc agcggcgcgc aggtgagcac
     2761 cgggatccgg ctgcgcacat ggaagtactc cagatcgcgc gtcccggcgg tgtggatgcc
     2821 acgcgcctcg atccgcagct gaccgtccgc cggctgtgcg gtgctgcatc ccagcatctc
     2881 gagcaggtca ctgaccagcc acgtgtcgac gatctgcgag atgttgtgga tcagcaccgg
     2941 ttcatcggtc agcaacgccg cgaccatcag cttgggaacc gcgtttttgg agccacggat
     3001 cttgaccgtg ccgccaagtg ggatgccgcc ggtcaggtgt agcgcgtcac cgtcatcagt
     3061 catggcgaag ccaagctcct gggtggttgt caggtccgac ttcgtagacg cgccgtgcgt
     3121 cgaaccggtt gtccggcgac gtcacggtac cgctgaggca gctgtaccgc gatgtcatcg
     3181 catgacctca tggggaacgc agcgccaaga atgggtgcag tgcatccggg ttgcgcaacg
     3241 cgccggagct cagcgcgcgc gccgggtcca tgccggcgag gatgttcttc accggcacct
     3301 cgcatttctt gccgttgagg gtgcggggca cttcgtcgat gacgatgaac cggttgggca
     3361 catgccgggg cgacaagcca cttcgcagcg ccgcacgcaa ccacggctcg acgtcaggca
     3421 gtgacacacc ggcggccagc accaggaagc acagcagctc accttggtca gcgcctgcac
     3481 ctgaggtgtc gatgaccagt gagtcgagca cttcgtcggc gccctcgacc accgcgtaga
     3541 actccgcggt gcccatccgc acaccgccgc ggttcagcgt ggcgtcggac cgaccgtaga
     3601 tcaccgacga gcctcgcggg gtgatcttga tccagtcgcc gtgccgccac aggcccgggt
     3661 acacctcgaa gtacgcctcg cgcaggcggg tgccgtccgg atcgttccag aagcagaccg
     3721 gcatcgacgg catcggcttg gtgatcacca gttcgcccac ctcgtcgatc agctcccggc
     3781 cgtcctcgtc gagggccgcc accgcggccc ccagcgcgcg gcaggagatc tcgccctccc
     3841 acaccggcac gtcaggcgcg gcacacagga aggccgcgca cagatcagtg cccccagaga
     3901 ccgaacagac ttgcacaccc gagccgatct ccgaggcgat ccagcggaaa ccctccgcgg
     3961 gcagcggggc accggtcgag cccaccgctc gcaacgcggt gagatccagc tcagcgccgg
     4021 gccgcaagcc ggccttgccg caggactgga cgaacggcgc ggacgtgccg aaatagttca
     4081 cgcgttcatc agcggccagt cgccacagcg aaccgaggtc ggggtgtgct gggctgccgt
     4141 cgtagagcac gatcgtcgcg cctaccagca ggccactgat caggtagttc cacatcatcc
     4201 agccggtggt ggtgaaccag aagaaccgct ggccgggccc gaggtcggct tgcaacgcca
     4261 gcgccctgag atgctccagg atgatcccgc cgtggccgtg cacgatgccc ttgggtaacc
     4321 cggtagtgcc cgaggagtag agcacccaaa gcggatggtc gaagtccacc gcctcgaacg
     4381 ccagctcagc accgcagtgc gcggcgagca gatcgtccca acacaccgtg gcatccaacg
     4441 tcacgctgga gtccaagtag gggaccagca ccgtggcgcg cagcgatggc agctgatcgc
     4501 gtagcgcgcc cactgtcgac cggatgtcga atcgtttgcc gccgtagagg tacccatcga
     4561 cggcgatcag cacggtgggc gtgagctggg tgaaccggtc gacgatcgct cgggttccga
     4621 aatccggcga gcacgatgac cagatcgcgc cgaggctggc ggtggcgagg aacgctacca
     4681 gcgtgtctgg cgagttcggc accagcgcga ccacccggtc gccccggcgg acacccagtg
     4741 acgccagtgc cgaccgcacc gcggccacct gctggcgcag caacccgaag ctccgcgcgt
     4801 cccgaacacc gtcctcgcga cggaagatca tggccgggtc atcgtcactc ttgccgggac
     4861 caggaaccag cgcgtgctcg gcatagttga gcgtcgcgcc cggaaaccac cgcgtcctcg
     4921 gcatcgactc accggtgagc acccgctgtg gcgcggtatg gaaccggacg ccggcaaaat
     4981 cggcgaacgc cccccagaac cccgccaggt cggccaccga ccagcgccac agcgcctcgt
     5041 agtcagccac gatgacgccc cgctgctccg ccaaccagct acgaaacgcc gccatccggg
     5101 accgggcgac ggcgtccggg tcgggcaacc agagaatctg gggctcggtc atgttcccgt
     5161 ggtaccaggg gtcgcgagtg cgtaacagtg ttctaacact gttacgcact cacgagcgcg
     5221 ctagcgcagg tggctgctga accagttcag ggtgcgaccc caggcttccg aggcgacgga
     5281 ctggtcggtg tggaaggagc agtgcaccag gtcggtggcg acgtgggcgg agtgggcggc
     5341 atctcgcagc ttggtcacct ccgcctcggg cacgtcacca tcctggtcgt aaatacctag
     5401 ccatgggcag cgcagctcag cggcaacgtc gaccagcgcg ggcagacccg ctgccaccgg
     5461 ctccactgcc ccgatcccgc caatggtcac cgccgcaccc agatcgcgtt tggtggcgac
     5521 gatgagcgcc gcggcgccac ctagtccgaa gccgaccacc cccatctggt cggcagccac
     5581 gcggcgccgc gccaaccagc gcaaggcggc gtcagtatcg gcgtgaacgg atgtttcggt
     5641 cagccgctcg agatggcggc gggcggtggc cccgccggcg ggtagctcgt caatgtcgtc
     5701 gcagtggaac agatctggga tcacggccag ccagccctct ccggccagcc cagcggcgag
     5761 ctgccagacg tcatcggtga cgcctccgac gtcaggctcg acgacgatgc ctccgcgcac
     5821 cgctgattcc ggcgtggcga ccgtcagccg cagcccagtg ccatcgcaga gcgggatctg
     5881 ttcgagacta cgttgcgtca tgtgtccacc cgcctcgtcc gcgaaaccag ggtgaggacg
     5941 cagcgtaccg ggtcgagagt tcggaagcct gatcacggtt tctggcacat aaaccgcgac
     6001 cacgtgcggc tgtggcgtca aacatcggcg acgggctcgc tacggtgatg ttggcgtgtt
     6061 ggccgcgcct ggttgcgaac aggaggcgcg atgaccgccc acaccagggc tgatctggag
     6121 tcgctgcccg ggtacgtccc tggtcgcagc atcccgggag cggtgaagct ggccagcaac
     6181 gaggtgtccg tgggtccgtt acccggcgtc gtcgcggcga tccgagacgc cgctgcgggc
     6241 atcaaccgtt acccggacaa cggggcggcc gcgctggtgg atcggttggc ggcccggttg
     6301 gacgtgccac cgtcgcaact cgccgtcgga gccgggtccg tggcactgtg ccagcagttg
     6361 gtgcaagcca cctgcactcc gaccgatgag gtgcttttcg cgtggcgctc cttcgaggcc
     6421 tacccgatca tcacccagat cgtcggagcc cggcagcgca cggtgccgct gcgcaccgac
     6481 cacacccacg atctggcggg gatgcttgac gcgatcaccc cagcgacccg gttggtcttc
     6541 gtgtgcaatc ccaataatcc gaccggcacg gccgtgcgac gcgacgagct ggtcacgttc
     6601 ctcgacgcgg tacccgacgg cgtgttagtc gccctggacg aggcctacca cgaattcgtc
     6661 accgatcccg cggtgcctga tggcatggag ctctaccggc agcgggaaaa cgtcgcggtg
     6721 ttgcggacgt tctccaaggc ttacgggctg gctgccctgc gggtggggta ctgcgtggcg
     6781 cccgagccgg tcgccacgac attgcgcaag gtgtccgtac cgttcagcat caactcatta
     6841 gcccaggtcg cagccctggc gagcctcgac gtcgaggacg agatgctcgc ccgctgccgc
     6901 agcgtggccg ctgaacggga ccgggttggt agcgagctga tcgcgatggg ctatcccgtg
     6961 ccgcgctcgc aggccaactt cgtctggctg cccttcggtg agcacaccat ggccttcaac
     7021 gagcactgct tggaacacaa ggtcgtcatc cgcccgttct ccggcgacgg cgtccgcatc
     7081 accatcggcg acccgaacga aaacgagatg gccctcgctg cggcgcgtga gtggcaaaac
     7141 agcgatccac aagggcgtca gcccccgata tagctcatct caaccttggg caaccctagc
     7201 gttgcggtgg tgcgctgggc tgagtaacgg tcggttcggt ccttccagat gccagtgatg
     7261 cgcttttcga tcgcatcatc gctggttccg tcgcgcagcg gcgtccggag gtcgtggccc
     7321 aggcccgcga acaagcaggt gtacaactcg cccttggcgg acaaccgagc ccgggtgcag
     7381 gtgccgcaga acggctggct gatcgaagtg atcacgccga cctcaccagc gccgtcggcg
     7441 tagcggtacc gagcggcgac ctcgccgacg taggccggat ccactggctc gataggccac
     7501 tgaccgttaa tggcctcgac gatctccgcc gccgcaacga cgtcgtccat ccgccagccg
     7561 ttggtggcgc cgacgtccat gaactcgatg aagcgcacga tgtagccgtg ttcccggccg
     7621 aaggcggcga ggtcgagaat gcccttgccg tcgttgagtc cacgcttgac caccgtgttg
     7681 agcttcaccg gcgtcagtcc ggcatccgcg gcggcggcga tgccctccag cacccgggac
     7741 accgggatct tcacgtcgct catggtccgg aacgtctgcg catcaaggga atccaggctc
     7801 accgtcaccc gggacagccc ggcatcgcgg agcgagcgcg ctttcttcgc cagcagtgag
     7861 ccgttggtag tcatggcgac gtcgtcgatg ccgtcgatgc cggccagcag ctcgacaagc
     7921 ccctcgatgc catgtcggag caacggctcc ccgcccgtga gtcgcagttt ttcaacaccc
     7981 agcccggcga agactttcgt cacccgaaca atctcttcga atgacagcag ttccgaggag
     8041 tccatgaacg gaaagtcggt gccgaaaacg tcgcgaggca tgcagtaaca acagcggaag
     8101 ttacaccgat ccgtaatcga gatccgcagg tcccgcaggc cccgtcccaa ctgatcgttc
     8161 acagtgttca cgccgtccat tggatcaggt tactcgggtg caccctttgt tctccttcgt
     8221 cggcacgcgt gagctggttc actgacagtc caacgcatcc ggattctggc ttatttcacg
     8281 ctactccatg tagctgaatc actcgttcga gttgttaacg aaccgagttc gcggagcgaa
     8341 aactagagtg actgctacca gtggtcaccg gcatgggatc ggtcgcggcg cgctgtttcg
     8401 cacactcctg gaggcttcgt gtccgcacct gcgtcgccat accggttcct tgccccgcaa
     8461 ccacgccatc agcggccgac gaagcgcgtc ggcgtcgtga gcttcacgct gggcctactg
     8521 gtcgtgttcg ggcttgtggt gtacgccact tggttaggtg tcgccgcact ggccaccgtc
     8581 gacggtgcca cccaacgcgt tggggcgact ggcaccacca cgcggctggc taccgccccg
     8641 ccaaaggctg cgccaccagc agtagtggtg actcccggcg gcaccacgat ggccttcgat
     8701 cagctgtgga ccgccagcga cgggaacacg atcgtcgccg gcgcgcccac ggtgggcact
     8761 tcgatgcaga ctggcgagcc ggtgattctg gtaccggtga cgctgaccaa taacggcgag
     8821 cgggactgga acccggtgta cacgtccttc gtcgggaaac tcaaccacgc gacggtgccc
     8881 gagtctgccg agggcgactg gatgtaccga tctccgatcg tcgcgcacac ctcggtgacg
     8941 ctgaccaagg tgttcgtcgg cggacccggc cagttcagct tgaccgcgaa caccccgaac
     9001 ggcgtcgcgt tgttcagcgg ccggatctga cttcgctggg tcttacgcag cccgacctac
     9061 ccgtctattg tgaggcaccc atagtgcttc aaccgtaggg gaatagtgac cgcgcctcgg
     9121 tcgagtgagg atccgagtgg tggggctggt tcggggcgat cgccggggcc gaccgcgctg
     9181 cgtatcgtgt tgggtactca actgcgtcgg ctgcgtgaag cgcggggtat caccgcagca
     9241 gcggccggcc acgcgatccg ggcgtcccac gcca
//
LOCUS       DASYLX010000331         7775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1989393,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000331 DASYLX010000000
VERSION     DASYLX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989393"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGP27_08065"
     CDS             complement(<1..336)
                     /locus_tag="VGP27_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851304.1"
                     /translation="MTVQTETDDASESSGPPVVASPTTGEAVRLFSREWFAGIALRYS
                     MVIIILLVIAYFSYRSARFSTVDNLVTILVAAAPFALIALGQTLVVLTGGIDLSVGSV
                     IAVSAMAAAA"
     gene            complement(333..1904)
                     /locus_tag="VGP27_08070"
     CDS             complement(333..1904)
                     /locus_tag="VGP27_08070"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020112717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV7851305.1"
                     /translation="MAQALLECADIKKSFGGVPVLKGITLYLEPGTVTALAGENGAGK
                     STLMKIVSGQYVADEGNVSVQGIHLAPGNTRDAVRHGVAIVPQELASIEDMTVYENLF
                     VGRELRRGPFLNRRAMIDQAAETLSAFDVAISPTARMGSLPVGLRQIVEIVKAARTGA
                     HVVMLDEPTSAISEREVEGLYAIVRRLREHGVAMVYTTHKMAEIQAIADRVVVLRDGV
                     LVMDESVADVSEDDIVTAMIGRELDELFPDRVEPTSEPVLQVRDLHVDGATAPVSLTV
                     NAGEIVGLAGLVGAGRTELLEAIFGARHTTAGEIVVRGHRVKRNSPAAAIAEGMAMVP
                     EDRKISGVVLSMSVLDNGSLPRLSSFSIAGWLRGKARTAAVTEAMESVRLRSSGMSQL
                     VGTLSGGNQQKVVLARWLTGEVNVLLLDEPTRGVDVGARSEIYRVIAQFAAAGMAVLM
                     ASSDMPEIIGLSHRAFVMRGGAFVGQLDRDALDHPAVQDSVFRMATSLKGATRAPDAP
                     ASIAEDRPESSEAAS"
     gene            1999..2724
                     /locus_tag="VGP27_08075"
     CDS             1999..2724
                     /locus_tag="VGP27_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-threitol dehydrogenase"
                     /protein_id="HEV7851306.1"
                     /translation="MSDHAVDLDFRLDDRVALVTGGASGIGAAIGDALAVKGARIAVA
                     DLAEGSEFHCDVRDPDSVTATVTAVVEAHGRIDILVNSAGIVRLGPAEELSVKDWEAT
                     IAVNLTGTFLMCQAVGAHMLAAGRGVIINMASQAASVALDAHIAYCASKFGVVGVSKV
                     LAAEWAGRGVRVNTISPTVVLTELGHQAWDGPKGDALKKLIPSGRFAYPDEIAAAAVY
                     LASDAAGMVNGADLIIDGGYTIK"
     gene            2813..3775
                     /gene="alc"
                     /locus_tag="VGP27_08080"
     CDS             2813..3775
                     /gene="alc"
                     /locus_tag="VGP27_08080"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEV7851307.1"
                     /translation="MADSEPDFTWLPDLALRPLGGAVIWANDETFAEKENLINQGPAT
                     FRPASFGHKGQIYDGWETRRRRDPGHDEAIVRLGVPGVIRGVVVDTAFFKGNYPPEVS
                     VEAIEVDGYPRPHELARDPKWETIVGRAKLYGDTRNPFEVSSEKRWTHVRLSIYPDGG
                     VARFRVHGECRPDHRFADVPGLDLAALENGGLVLDCSNRFYSSPQNLIFPGVANVMGD
                     GWETARRRDDGNDWVHIRLAGRGVVRMAEIDTSYFIGNSPGAARLSGLRRDGQWVELL
                     PRTELLADTRHRFPVESDAVISEARLDIYPDGGLARLRLFGELA"
     gene            3790..4689
                     /gene="puuE"
                     /locus_tag="VGP27_08085"
     CDS             3790..4689
                     /gene="puuE"
                     /locus_tag="VGP27_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEV7851308.1"
                     /translation="MVGYGRTPPDARWPDGAAIAVQFVLNYEEGAENTVLDGDPASEA
                     FLSEITPAEAFPNRHMSMESLYEYGSRAGLWRLLGVFERRRIPLTIFAVARAMQRNPE
                     AVAAFGELGHEIACHGLRWKSYQLIGRDTERMHMAEAVQILKDLTGAAPLGWYTGRDS
                     PHTRDLVVKHGGFVYDSDSYADDLPYWVRVHDGADLVDHLVVPYTLDTNDMRFASPAG
                     FSNGDEFFAHLRDAFDVLYAEGLDGSPKMLSVGLHCRLAGRPARTRALERFLDHVQSH
                     DSVWLARRIEIAEHWRRIHPPAQ"
     gene            complement(4671..5387)
                     /locus_tag="VGP27_08090"
     CDS             complement(4671..5387)
                     /locus_tag="VGP27_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV7851309.1"
                     /translation="MTQIWVINPNTTEAMTAAITRCARAVVAPGTTVTGITPESGPPS
                     IESHYDEAMSVPGLLAAIGLGEDEDVDGYVIACFGDPGLDAAREVAGGPVIGIAEAAM
                     HTASHLGRSFSVVTTLARTVGRAEELTERYGMQRFCRGVHACEIPVLDLDTNPDARKI
                     VTEACREAVEVDGSEVLVLGCAGMAEMCGPISAELGVPVVDGVTAATLTVQSLVAMGL
                     ATSKRGEYASPAKKDYWAGG"
     gene            complement(5384..6940)
                     /locus_tag="VGP27_08095"
     CDS             complement(5384..6940)
                     /locus_tag="VGP27_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEV7851310.1"
                     /translation="MTDIDTPQTTKDLPPGVVVGAGDLIEASGHPVGGGVIKAGYDPR
                     LTNEDLAPLGKQTWSSYNIFAFWMSDVHSVGGYVTAGSLFALGLASWQVLVALLIGIV
                     IVNVFCNLVAKPSQATGVPYPVICRSVFGVLGANIPAIIRGLIAVAWYGIQTYLASAA
                     LDIVLLKLFPGLAPYSDVEQYGFLGLPLLGWGSFILLWILQACVFWRGMEAIRKFIDF
                     CGPAVYVVMFLLCGYLIYKAGWGAIDLNLGEVTYTGFESIPVMLGAIALVVSYFSGPM
                     LNFGDFARYGKSFAAVKKGNFLGLPVNFLVFSLLVVVTASLTMPVFGELITDPVQTVA
                     RIDSTFAIVLGALTFTIATIGINIVANFISPAFDFSNVSPQRISWRAGGMIAAVGSVL
                     ITPWNLYNNPEVIHYTLETLGAFIGPLFGVLIAHYYLVHKQKVVIDDMFTLDENGTYW
                     YKKGYNPAAVITTIVAAVTAVVPVLLGGYVVGMHTAAQYSWFIGCGIGFGLYYLLATR
                     STKMAVAPIP"
     gene            complement(6994..7719)
                     /locus_tag="VGP27_08100"
     CDS             complement(6994..7719)
                     /locus_tag="VGP27_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7851311.1"
                     /translation="MPSRRRSALVDKLVVDQPGRPQQAILDELRRVILDGAVPPGTPI
                     PLADVADLFGVSQIPVRESLKTLIGEGLVSHRSNAGYAVAQLTSQELREMYIVRETLE
                     AASLAAAIANATDEDRAAVVAANDVLQQAIRDEDPATYHRQSRNFHLALTRPSRMYRL
                     LRMLESAWNVTEPVQSMVHVRPADRAELHADHCQMLEAFLASDVERLLTVAELHAQRL
                     NAVIATLPTDTGLLVSGDISSAQ"
BASE COUNT         1329 a         2593 c         2551 g         1302 t
ORIGIN      
        1 tgcggcggcg gccatcgccg acaccgctat cacgctgccc accgatagat cgatgccgcc
       61 ggtcagcacg accagcgtct ggcccagagc gatgagcgcg aacggcgccg ccgcgaccag
      121 gatggtgacc aggttgtcga cggtgctgaa acgcgcactg cggtaactga agtacgcgat
      181 gaccagcagg atgatgatga ccatcgagta acgcagggcg ataccggcga accactcgcg
      241 ggagaacaac cgaacggctt cgccggtggt gggcgacgcg acgaccggcg gccccgagga
      301 ctccgatgcg tcgtcggttt cggtttgcac tgtcacgatg ctgcctccga tgactcgggc
      361 cggtcctcgg cgatggatgc gggcgcgtcc ggcgcgcggg tggcaccttt gagcgaagtc
      421 gccatccgga acacggagtc ctggaccgcg ggatgatcga gcgcgtcccg gtcgagttgg
      481 cccacgaagg cgccgccacg catgacgaac gcccggtgcg agaggccgat gatctccggc
      541 atgtccgacg atgccatcag cacggccatg ccggccgcgg cgaactgggc gatgacgcgg
      601 tagatctcgc tgcgcgcgcc gacatccact ccgcgtgttg gctcgtcgag cagcaggacg
      661 ttgacctcgc cggtgagcca ccgggccagc accaccttct gttggtttcc gcccgacagc
      721 gtccccacga gttgactcat cccactactg cgcaaccgca ccgattccat cgcttcggtg
      781 acggcggcgg tccgcgcctt gccgcgcagc cagccggcaa tgctgaacga cgacagccgg
      841 ggcagtgatc cgttgtcgag cacgctcatc gacaacacca cgccggagat cttgcgatcc
      901 tcgggcacca tcgccatgcc ttccgcgatt gcggcggccg gcgaatttcg cttgacccgg
      961 tgtccgcgca ccacgatctc gcctgcggtg gtgtgtctgg cgccgaagat cgcctccagc
     1021 agttcggttc gtccagcacc gaccaggccg gcgagcccga cgatctcccc ggcgttcacc
     1081 gtgagcgaca ccggcgcggt ggcaccatcg acgtgcagat cgcggacctg tagcacgggt
     1141 tcggaggtcg gttcgacgcg atcggggaag agctcgtcca gttctcggcc gatcatcgcg
     1201 gtgacgatgt cgtcctcgga cacgtcggcc accgactcgt ccatgaccag gaccccgtcc
     1261 cgcaggacaa cgacgcgatc cgcgatcgcc tggatctcgg ccatcttgtg ggtggtgtaa
     1321 accatcgcga ccccgtgctc gcggaggcgg cgcacgatcg cgtacaggcc ttcgacctcg
     1381 cgctcggaga tggccgacgt cggctcgtcg agcatcacga catgcgcacc cgtgcgggca
     1441 gccttcacga tctccacgat ctgccgcaga ccgaccggaa ggctgcccat ccgtgcggtg
     1501 ggcgagatcg ccacgtcgaa cgccgacagc gtctcggcag cctggtcgat catcgcccgg
     1561 cggttcagaa acgggccccg gcgcagttcc cggccgacga agaggttctc atagaccgtc
     1621 atgtcctcga tcgaagccag ctcctgcggc acgatggcta cgccgtgccg taccgcatcg
     1681 cgggtgttgc cgggggcgag gtggatgccc tgtaccgaca cgttgccctc gtcggcaacg
     1741 tactggccgc tgacgatctt catcagcgtg gacttgcccg cgccgttttc gcctgccagt
     1801 gcggtgacgg tgccgggttc gaggtacagc gtgatgccct tcaatacggg aacgccgcca
     1861 aagctcttct tgatgtccgc gcattccagt aatgcctgcg ccaccgagat ctcccttgat
     1921 cgatgccctc gcggacagac gttacgttcc caatcgcgac gaggtgtaac catcgtcaca
     1981 atagggtaat ttcgatcggt gtccgatcac gcagtggatc tcgacttccg cctggacgac
     2041 agggttgccc tcgtcaccgg tggcgcatcg ggaatcggtg ccgccatcgg cgatgcgctg
     2101 gccgtcaagg gcgcccgaat cgcggtcgcg gatctcgccg aaggtagcga gttccattgc
     2161 gacgtccgcg atcccgactc cgtgaccgca acggtcactg cggtcgtcga agcgcacggt
     2221 cggatcgaca tcctggtcaa cagcgcgggc atcgtgcggc tcggccccgc cgaggagctg
     2281 tcggtgaaag actgggaagc caccatcgcg gtgaacctca cgggtacgtt tctgatgtgc
     2341 caggcagtcg gcgcgcacat gctcgccgcc ggtcgcggcg tcatcatcaa tatggcgtcc
     2401 caggccgcca gtgtcgcgct cgacgcgcac atcgcgtatt gcgcctcgaa gttcggtgta
     2461 gtcggcgtct ccaaagtcct tgccgctgaa tgggcgggcc ggggcgttcg ggtcaacacc
     2521 atctccccca cggtggtact gaccgagctc ggacaccagg cgtgggacgg acccaagggg
     2581 gacgcactca agaagctcat accctcgggc cgcttcgcct accccgacga gatcgccgcg
     2641 gccgcggtct atctggcctc ggacgccgcg ggcatggtca acggcgccga cctgatcatc
     2701 gacggcggct acacgatcaa gtaggtgcgc ggccgtggcg ccgactcgat gcggacatca
     2761 ccaaaacact gggcgcgctg cgagggcgta aaccttctgt gacgatggtc gggtggcgga
     2821 ctcggaaccg gacttcacct ggttgcccga ccttgccctg cgccccctcg gcggtgcggt
     2881 gatctgggcg aacgatgaga cgttcgctga gaaggagaac ctgatcaacc agggcccggc
     2941 tacgtttcgg cctgcgtcgt tcggccacaa gggtcagatc tacgacgggt gggagactcg
     3001 tcgcaggcgg gaccccggtc acgacgaggc gatcgtgcgt ctgggtgtgc ccggcgtgat
     3061 ccgcggcgtc gtcgtcgaca ccgccttctt caagggcaac tacccgccgg aggtgtcggt
     3121 cgaagccatc gaggtcgacg gctatccccg gccgcacgaa ctggcccgcg acccgaaatg
     3181 ggaaaccatc gtggggcgcg ccaagttgta cggcgacacc cgcaacccgt tcgaggtgtc
     3241 ctccgagaag cgttggacgc acgtgcgttt gagcatctac cccgacggtg gcgtggcgcg
     3301 gtttcgggtg cacggcgagt gccgtcccga ccaccggttc gccgacgtgc cgggcctcga
     3361 cctggccgcg ctggagaacg gcggcctcgt gctggactgc tcgaaccgct tctacagctc
     3421 cccgcagaac ctcatcttcc ccggcgtcgc caatgtcatg ggcgacggct gggaaacggc
     3481 tcgccggcgc gacgacggca acgactgggt gcacatccgg ctggccggtc gtggcgtggt
     3541 gcggatggcg gagatcgata cgtcgtactt catcggtaac agccctggcg cggcgcgctt
     3601 gtcgggtctg cgccgcgacg gccagtgggt cgaactgctg ccgcgcaccg agcttctggc
     3661 cgacacccgc caccggttcc ccgtcgagtc ggatgcggtg atctccgagg cccgcctgga
     3721 catctacccc gacggcgggc tggcccggct acggctgttc ggtgagctgg cgtgagctac
     3781 ccccgcgaca tggtcggcta cggccgcacc ccgccggacg cacggtggcc cgacggcgcc
     3841 gcgattgccg tgcagttcgt tctcaattac gaagagggcg ccgagaatac ggtgctcgac
     3901 ggcgaccccg cctccgaggc gtttctctcg gagatcaccc ccgcggaggc cttccccaac
     3961 aggcacatga gcatggaatc gttgtacgaa tacggctccc gggccgggtt gtggcgcctg
     4021 ctcggggttt tcgaacgacg acggatcccg ctgacgattt tcgcggtggc acgcgccatg
     4081 cagcgcaacc cagaagccgt cgccgcgttc ggcgaactgg gccacgagat cgcctgccac
     4141 gggctgcggt ggaagtccta tcaattgatc ggccgcgaca ccgaacgcat gcacatggcc
     4201 gaggccgtgc agattttgaa ggacctcacc ggcgccgcac cactgggctg gtacaccggt
     4261 cgtgactccc cgcacacacg cgacctggtc gtcaaacacg gcgggttcgt ctacgactcg
     4321 gactcctacg ccgacgacct gccctactgg gtccgggtgc acgacggtgc cgatctggtg
     4381 gaccatctcg tcgtccctta cacactcgac accaacgaca tgcggttcgc ttctcccgca
     4441 ggcttttcca acggcgacga gttcttcgcg catctccgcg acgcattcga cgtactctat
     4501 gccgaggggc tcgacggcag cccgaagatg ttgtcggtcg gcctgcactg ccggctcgcg
     4561 ggcagacctg cccgcacgag ggcgctggag cggttcctcg atcacgtgca atcccacgat
     4621 tcggtgtggc tggcccggcg catcgagatc gccgaacact ggcgccgtat tcacccgcct
     4681 gcccagtagt ccttcttcgc aggcgaggcg tactcgccgc gcttcgacgt ggcaagcccc
     4741 atcgcgacca gcgactggac ggtcagcgtc gcagcggtca ccccgtcgac caccggcacg
     4801 cccagctccg ccgagatggg cccacacatc tcggccatac ctgcacaacc cagcaccagc
     4861 acctcgctgc cgtcgacctc caccgcttcc cggcatgcct cggtgacgat cttgcgcgcg
     4921 tcgggattgg tgtcgaggtc gagcaccggg atctcgcagg cgtggacgcc acggcagaac
     4981 cgttgcatgc catagcgttc ggtgagttcc tcggcgcgcc cgacggtgcg cgccaacgtc
     5041 gtgaccacgc tgaagctccg gccgagatgg ctggcggtgt gcatggcggc ctcggcaatg
     5101 ccgatcaccg gtccccccgc cacctcgcgg gcggcgtcca ggccagggtc gccgaagcag
     5161 gcgatgacgt agccgtcgac gtcttcgtct tcgcccaggc cgatcgcggc cagcagaccc
     5221 ggcacgctca tcgcctcgtc gtagtggctt tcgatggacg gcgggccgct ttccggcgtg
     5281 atgccggtga ccgtggtgcc gggtgcgacg accgcccgcg cgcagcgcgt gatcgctgcg
     5341 gtcatcgcct cggtggtgtt cgggttgatg acccagatct gcgtcacgga atcggtgcca
     5401 ccgccatctt ggtgctgcgg gtggcgagca ggtaatacag gccgaagccg atgccgcagc
     5461 cgatgaacca gctgtactga gcggctgtgt gcatgccgac gacgtaaccg cccaacagca
     5521 cggggacgac cgcggtcacc gccgcgacga tcgtcgtgat caccgccgcc gggttataac
     5581 ccttcttgta ccagtaggtt ccgttctcat cgagcgtgaa catgtcgtcg atgaccacct
     5641 tctgcttgtg caccaggtag tagtgggcga tcagcacacc gaacagcggg ccgatgaagg
     5701 cgccgagggt ctcgagcgtg tagtggatga cctcggggtt gttgtacagg ttccacggcg
     5761 ttatcagcac cgagccgacg gcggcgatca tgccgcccgc gcgccagctg atccgctgcg
     5821 ggctgacgtt ggagaagtcg aacgccggcg agatgaagtt ggcgacgatg ttgatgccga
     5881 tggtggcgat cgtgaacgtc aaggcaccca acacgattgc gaacgtcgag tcgatgcggg
     5941 ccaccgtctg gaccgggtcg gtgatgagct caccgaacac gggcatggtc agtgacgccg
     6001 tgacgaccac cagcagcgag aagaccagaa agttcaccgg taggccgagg aagttgccct
     6061 tcttcaccgc tgcgaaggac tttccgtagc gcgcgaagtc gccgaagttg agcatcgggc
     6121 cggagaagta ggacaccaca agcgcgatgg cgccgagcat caccggaatg gattcgaagc
     6181 cggtgtaggt gacctcaccg aggttgagat cgattgcgcc ccagcccgct ttgtagatca
     6241 gatagccgca gagcaggaac atcaccacgt acacggcggg cccgcagaag tcgatgaact
     6301 tgcggatggc ttccatgccg cgccagaaca cgcaggcctg caggatccac agcaggatga
     6361 agctgcccca gccgagcaga ggcagcccga ggaacccgta ctgctccaca tcggagtagg
     6421 gcgccagccc gggaaacagc ttgagcagca cgatgtccag ggctgccgag gccaggtagg
     6481 tctggatgcc gtaccaggcc accgcgatca ggccgcggat gatggccggg atgttggcgc
     6541 cgagcacccc gaagacgctg cgacagatca ccgggtacgg cactccggtg gcctggcttg
     6601 gcttggcaac cagattgcag aagacgttga cgatgacgat gccgatcagc agggcgacca
     6661 gcacctgcca actcgccagc ccgagcgcga agaggctgcc cgccgtcacg tagccgccga
     6721 cgctgtgcac atcggacatc cagaacgcga agatgttgta ggacgaccag gtctgcttgc
     6781 ccagcggcgc caggtcctca ttcgtcaggc gcgggtcgta gcccgccttg atgacgccac
     6841 ccccgacggg gtgacccgaa gcctcgatca gatcgcctgc gccgacgacg acgcccggcg
     6901 ggagatcctt tgtggtctgc ggtgtgtcga tgtccgtcat gcgcggacgc tatcgacggc
     6961 gtatttcgct gcggtttcct gcgaaattcc ccactattgc gccgaagata tatctcccga
     7021 caccagcagt ccggtgtcgg tgggcagcgt cgcaatgact gcgttcagcc gctgagcatg
     7081 gagttcggcc accgtgagta gtcgctcgac gtcactcgcg agaaacgcct cgagcatctg
     7141 acagtgatcg gcgtgcaact cggcgcggtc cgcgggccgg acatggacca tcgactgcac
     7201 cggttcggtg acgttccacg ccgattcgag catgcgcaga aggcggtaca ttcgcgacgg
     7261 ccgggtcagt gccagatgga agttccggct ctgccggtgg taggtcgccg gatcctcgtc
     7321 gcggatcgcc tgctggagaa cgtcgttggc cgcgacgacg gccgcgcggt cctcatcggt
     7381 cgcgttcgcg atcgcggccg ccagcgatgc cgcctccaat gtctcacgga cgatgtacat
     7441 ttcgcgcagc tcctggctgg tgagctgggc gacggcatag cctgcgttgc tgcggtgcga
     7501 caccagcccc tcaccgatca acgtcttgag cgactcgcgg accggtatct ggctgacgcc
     7561 gaacagatct gcgacgtcgg ccagtgggat cggggtcccc ggtggcaccg ccccgtcgag
     7621 gatcacccgg cgcagctcgt cgaggatggc ctgttgcggc cggccgggct gatcgacgac
     7681 gagcttgtcc accaacgccg agcgtcgtcg agacggcatc ccgacacggt aagcagcgtt
     7741 tgtttcgcca gtgttgctac agcgaggcgg cgacc
//
LOCUS       DASYLX010000332         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_234002,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000332 DASYLX010000000
VERSION     DASYLX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234002"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VGP27_08105"
     CDS             complement(<1..753)
                     /locus_tag="VGP27_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019684071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7851312.1"
                     /translation="MGPVGASSPLAVRMRPASLDEVLGQDHLLQPNSPMRRLVEGSGA
                     ASVILYGPPGTGKTTLASLISQSTGRRFEALSALSAGVKEVRAVIEKARVALLRHGEQ
                     TVLFIDEVHRFSKTQQDALLSAVENRVVLLVAATTENPSFSVVAPLLSRSLILQLQPL
                     TPDDVRVLVARAIEDPRGLGGKVKVTDEAVELLVQLSAGDARRALTALEVASEAGEQV
                     TVETIEQSLDKAAVRYDRDGDQHYDVISAFIKS"
     gene            complement(828..1664)
                     /locus_tag="VGP27_08110"
     CDS             complement(828..1664)
                     /locus_tag="VGP27_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HEV7851313.1"
                     /translation="MADRYGTDVLADNPHRKPRSTEVPLEMGMVVEDAQTGYVGAVVR
                     IEYGRMELEDRRGRRKPFPVGPGYLIDGKPVILAAPRRAAAASSTRTSSGSVAVPGAK
                     ARVALSSRIYVEGRHDAELVEQVWGDDLRIEGVVVEYLGGVDDLVGIVEEFRPGPARR
                     LGVLVDHLVAGSKEARIADAVRRSSGGSHTLVVGHPFIDIWQAVKPDRLGIAAWPSIP
                     RGVDWKHGVCDALGWPHAQQADIARAWKRIRGQVRDWNDLEPALIGRVEELIDFVTQC
                     ES"
     gene            1688..2263
                     /locus_tag="VGP27_08115"
     CDS             1688..2263
                     /locus_tag="VGP27_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851314.1"
                     /translation="MLLNRDTAEGIANGKVTLVLRRWDAPRAKPGGTQRTPAGTICID
                     AVTEYPGSHRVTAAQARAAGYPDAKSAQADLDRRTARHTYAIEVSYLAPDERPQLAAD
                     DRLSESDVDAIGARLQRWDAAGEAPWTRMYLELIAANEAVRAPELASQVGLDTARFKR
                     RVRRLKGLGLTISLDVGYRISPRGRAYLDAS"
     gene            complement(2260..3126)
                     /locus_tag="VGP27_08120"
     CDS             complement(2260..3126)
                     /locus_tag="VGP27_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEV7851315.1"
                     /translation="MSVPPVPWPNGARCAASFSFDVDAESAMLGVDASHADRMSAISH
                     QAYGPLTGVPRLLKVLAKHDVTSTFFVPGYTAVRYPDVIRSIVDHGHEIAHHGYLHEP
                     MRGKTEQEEAVILDRASEVLESVTGSRPTGYRAPMWELNWHSPKLLRDRGFLYDSSLM
                     DTDHPYELEVAGGGPLVEIPIQWALDDWEQYCYVPEFSGSGLIESPVKAIEMWKLEFD
                     AMRAEGGAYVLTNHPFLSGRPSRAQALDSLIEYVCSHDDVWVTGLGTIATHVRGLGLT
                     PRSVDRPNPADF"
     gene            complement(3123..4079)
                     /gene="speB"
                     /locus_tag="VGP27_08125"
     CDS             complement(3123..4079)
                     /gene="speB"
                     /locus_tag="VGP27_08125"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEV7851316.1"
                     /translation="MSDNQPVQPVDGTVVPRYAGFSTFARLPRIGDVPSYDVAVVGVP
                     FDTGVSYRPGARFGPAHVRQCSRVLRPYHPQMQVSPFAAQQVVDAGDIACTPYDIAKA
                     VQQVEEQASALMADGARLITIGGDHTIAYPLLRAHHRRYGPVALVHFDAHLDTWDTYF
                     DAPLTHGTPFRRAAEEGLFVSGHSAHVGIRGSLYSPDDLSSDAELGFTIVHCREFQTR
                     TVADVVAQLRDAIGDHPLYVSIDIDVLDPAHAPGTGTPEIGGLTSRELLEVVRGFEGL
                     NLVGADIVEVAPAYDHAELTGIAAAYLAYEFLALLAGARSRS"
     gene            complement(4076..4843)
                     /locus_tag="VGP27_08130"
     CDS             complement(4076..4843)
                     /locus_tag="VGP27_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851317.1"
                     /translation="MREGHHRGRIALVTGATQGIGAAIAERLADEGAAVGVNGRVRDH
                     RMDGVVAATGGFPAAADVSDPDAVAGMVADIERSRGPVDILVCNAAYMTMAPFTDHAD
                     EDWWKILDTNLSGTVYLVQAVLAGMRRRGGGNIVIIASEWGVVGWPNATAYAGSKAGL
                     IALTKSLGRELAPEHITVNGIAPGIVDTPQLQVDADDAGLTLAEMHREYERAIPLGRI
                     GKPKEIAAAVSLLARRDLGAFVGQTIQINGGTTRCRA"
     gene            complement(4843..5526)
                     /locus_tag="VGP27_08135"
     CDS             complement(4843..5526)
                     /locus_tag="VGP27_08135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851318.1"
                     /translation="MSGVAVVTGASSGIGAAIAAALEQDGYRVAGFDLDESAPYSVDV
                     ADPAAVEVTMRRVATELGPVDAVVSAAGYYEETPIADITHGQLHRMLRVHLGGLRNVA
                     RAALPSMIERRRGSIVAITSELAVGGGDGEAHYAAAKGAIIGLVRSLAAEVAAHGVRV
                     NAVAPGPTDTPLLTPDSPWRAPEYLATLPARRLARPGEIAEVVRYLVSDDAGFCTGEV
                     LSPNSGAVI"
     gene            complement(5523..6983)
                     /locus_tag="VGP27_08140"
     CDS             complement(5523..6983)
                     /locus_tag="VGP27_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7851319.1"
                     /translation="MAESIDTNTVSEPAPERLQRGFSFRSALSLAFADVSPIAAVYAI
                     FTLGLFAAGPKFFWAFPIVLIGQLMVAAVFGELASRWPYAGSVYQWSRHVQGTTWGWV
                     AAWAYMWGLTLALGTLSYAAAGFLLNIFGVGEPNRWQIASVAIAIVLVGSAVNMVGRR
                     VLKIMVAISITCEVVGSLGLGVVLLLFYRENPFSVLFSSAGIPDAASWASGPMLLAIA
                     YVGWSFLGFEAAGSVAEEVDEPERNVPKAIILALLLVGLVVMFSAAALILSIPNLPGV
                     VAAQSGDVVSDTLTTHLGEGVTKPLLAMFVIGFVSSFLAVQAAVSRCIWGAARDRSLP
                     GSKVLGGLAGPERLPVNAIALTAVAAIVFVLLAGTQFYNILVNFNIIGFYIAFGVPVI
                     GAAISRLSGKWQPGPFTLGRWGAPITYIASLWIIFETVNVAWPRTSPDVPWYISWASV
                     LTTVVLGLIGAAIYLSVRRNIQAPIAERFADKPPPQ"
BASE COUNT         1279 a         2520 c         2436 g         1115 t
ORIGIN      
        1 ggatttgatg aacgcgctga tcacgtcgta gtgctgatcg ccgtcacggt cgtagcgaac
       61 cgcggccttg tcgagcgact gctcgattgt ctcgacggtg acctgctccc ccgcctccga
      121 cgcaacctcg agcgcggtca gcgctctcct ggcatcgccg gccgacagct gcaccagcag
      181 ctccaccgcc tcgtcggtga ccttgacctt tccgccgagc ccgcggggat cctcgatggc
      241 acgggcgacc aatacccgga cgtcgtcggg ggtcagtggt tgcaactgca ggatcagcga
      301 ccgcgacagc aacggcgcga cgaccgagaa cgacgggttt tcggtggtgg cggcgaccag
      361 cagcaccacc cgattctcga cggccgacag cagcgcgtcc tgctgggtct tggagaatcg
      421 gtgcacctcg tcgatgaaca acacggtctg ttcgccgtgc cggaggagcg cgacgcgtgc
      481 cttttctatg acggcgcgga cttccttcac cccggcagac agcgcggaaa gcgcctcgaa
      541 ccggcggccc gtcgactgcg agatcagcga cgccagcgtg gtcttgccgg tgccgggcgg
      601 gccgtagagg atgaccgacg ccgcacccga tccctccacc aggcggcgca tcggcgagtt
      661 cggctgcagc aggtgatcct ggccgaggac ttcgtcgagc gaggccgggc gcatccgcac
      721 cgccagcggg ctcgacgcac ccacgggccc catcgcggtt cccgtcgagg tcggctcgcc
      781 tgggacgtca aacagaccgt cggacacgct ctctgcttac cacgccgcta cgactcgcat
      841 tgcgtgacga agtcgatcag ttcctcgacg cggccgatga gcgcgggttc caggtcattc
      901 cagtcgcgca cctggccgcg gattcgcttc caggccctgg cgatgtccgc ctgttgggca
      961 tgcggccagc cgagtgcatc gcacaccccg tgcttccagt ccacaccgcg cgggatcgat
     1021 ggccacgccg cgatgccgag cctgtccggc ttgaccgcct gccagatatc gatgaacggg
     1081 tggcctacga cgagcgtgtg cgaaccccct gagctgcgcc gaaccgcgtc ggcgatgcgc
     1141 gcctccttgg agccggcgac caagtgatcg accagcacgc ccagcctgcg cgccggccct
     1201 ggccggaact cctcgacgat gccgaccaga tcgtcgaccc cgccgaggta ttcgacgacc
     1261 acgccctcga ttcgcagatc gtctccccat acctgctcga ccagttcagc gtcatggcgg
     1321 ccctcgacgt agatgcggct ggacagcgcc actcgcgcct ttgcgcccgg caccgcaacc
     1381 gatccggacg aagtgcgggt gctcgacgcc gcggccgccc gccgcggcgc ggccaggatc
     1441 accggcttgc cgtcgatcag gtaaccggga ccgacgggaa acggcttgcg gcgaccgcgc
     1501 ctgtcctcga gttccattcg gccgtactcg atacggacca ccgcgcccac atagccggtc
     1561 tgcgcgtcct ccaccaccat gcccatctcg agaggcacct cggtggatcg cggttttctg
     1621 tgcggattgt cggcgaggac atccgtgcca tagcggtcag ccaccgggtg atcgtagggt
     1681 cacacccgtg ctgctgaacc gcgacacggc cgagggaatc gcgaacggaa aggtcacgct
     1741 tgtgttgcgc cgctgggatg ctccgcgcgc caagcccggt ggcacacaac ggacaccggc
     1801 gggcacgatc tgcatcgacg ccgtcaccga atacccgggc tcccatcggg tgaccgccgc
     1861 gcaggcgcgc gcggcaggct atcccgatgc gaaatccgca caggcagatc tcgaccgcag
     1921 gaccgcgcgg cacacctacg cgatcgaggt gtcctatctc gcgcccgacg aacgcccaca
     1981 actggccgcc gacgaccgcc tcagcgagtc cgacgtcgac gccatcggcg cgcgcctgca
     2041 gcgatgggac gccgccggtg aagcaccgtg gacgcggatg tatctcgagc tcatcgccgc
     2101 caacgaagcg gttcgcgctc ccgaactggc ctcccaggtc ggcttggaca cagcgcggtt
     2161 caagcgcagg gtacgccggc tcaagggcct cggcctgacc atcagcctcg acgtcggata
     2221 ccgaatctcc ccacgtgggc gtgcatatct ggacgcgtcc tagaagtccg cggggttcgg
     2281 ccggtcaacc gagcgcggcg tcagaccgag accgcgcacg tgcgtcgcga tcgttcccag
     2341 gccggtgacc cacacgtcgt cgtgcgagca cacgtactcg atgagcgaat ccagagcctg
     2401 tgccctcgac ggccgccccg acagaaacgg atgattggtc agcacatacg cgccgccctc
     2461 ggcgcgcatc gcgtcgaact ccagcttcca catctcgatt gccttgacag ggctttcgat
     2521 caggccgctg ccgctgaact cgggcacgta gcagtactgc tcccagtcgt cgagcgccca
     2581 ctgaatggga atctccacaa gcgggccgcc gcccgcgacc tccagctcat aggggtggtc
     2641 ggtatccatc aggctgctgt cgtagagaaa tcccctgtcg cgcagcaact tcggcgagtg
     2701 ccaattcaac tcccacatcg gcgcacgata gcccgtcgga cgcgagccgg tcaccgactc
     2761 cagcacctct gacgcccggt cgaggatgac cgcctcctcc tgctcggtct tgccgcgcat
     2821 gggctcgtgc aggtaaccgt ggtgtgcgat ctcatggccg tggtcgacga tcgagcggat
     2881 cacgtccgga tagcgcacgg cggtgtagcc gggaacgaag aacgtcgacg tgacgtcatg
     2941 cttggcaagg actttcagca gccggggcac cccggtgagc ggaccgtagg cctggtggct
     3001 gatcgcggac atccggtcgg cgtgtgaggc atcgacaccc agcatcgccg actccgcgtc
     3061 gacgtcgaac gagaacgacg ccgcgcaacg ggcgccgttc ggccacggaa ccggtggcac
     3121 gctcacgacc gactccgcgc accggccagc agagcgagga actcgtatgc caggtacgcg
     3181 gcggcgatac cggtcaactc tgcgtggtcg taggcgggcg ccacctcgac gatgtcggcc
     3241 ccgacgaggt tgaggccctc gaacccgcgt accacctcga ggagttcgcg gctggtcagg
     3301 ccgccgatct cgggtgtgcc ggtgccgggg gcatgggccg ggtcgagcac gtcgatgtcg
     3361 atcgacacgt agagcggatg atcgccgatg gcgtccctca gctgcgcgac cacatctgcg
     3421 acagtacgtg tttggaactc tcggcaatgg acgatggtga acccgagttc ggcgtcggac
     3481 gacaggtcgt cgggcgaata gagagaaccg cggatgccga cgtgcgcgct gtgccccgat
     3541 acgaagagac cctcctccgc ggcgcggcgg aacggtgtgc cgtgtgtgag cggcgcgtcg
     3601 aagtaggtgt cccaggtgtc gaggtgcgca tcgaagtgca cgagtgccac cggaccgtag
     3661 cggcgatgat gcgcccgcag cagggggtac gcgatcgtat gatcgccgcc gatcgtgatg
     3721 aggcgtgcgc cgtcggccat cagcgccgac gcctgctcct cgacctgctg gaccgccttg
     3781 gcgatgtcgt atggcgtaca agcgatgtcg cccgcgtcga ccacctgctg ggctgcgaac
     3841 ggcgaaacct gcatctgggg gtggtatggc cgcagcacgc gtgagcactg ccggacatgg
     3901 gccgggccga accgcgcacc cggccgatag ctgacaccgg tgtcgaacgg aactccgacg
     3961 accgcgacgt cgtagctcgg cacgtcgccg atgcggggca gccgcgcgaa cgtggagaag
     4021 ccggcatagc gcggaacgac ggtgccgtcg accggctgga ccggctgatt gtcgctcatg
     4081 cccgacatcg tgtcgtccct ccgttgatct ggatggtctg gccgacgaac gcaccgaggt
     4141 ctcggcgggc caacagggag accgcggcgg cgatctcctt cggtttgccg atgcggccga
     4201 gcggtatcgc gcgctcgtac tccctgtgca tctcggcgag ggtgagaccc gcatcgtcgg
     4261 cgtcgacctg aagctgcggg gtgtcgacga tcccgggggc gattccgttg accgtgatgt
     4321 gctcgggcgc cagctcgcgc ccgagtgatt tcgtcaacgc gatcagaccg gcctttgacc
     4381 cggcgtacgc ggtggcattc ggccacccga ccacacccca ttcggatgcg atgatgacga
     4441 tgttgccgcc acctcgccgc cgcatgccgg cgagaacggc ctgcaccagg taaaccgtgc
     4501 cggacaggtt ggtgtcgagg atcttccacc agtcctcgtc ggcgtgatcg gtgaacggcg
     4561 ccatggtcat gtaggccgca ttgcacacca ggatgtccac cggcccgcgg ctgcgctcga
     4621 tgtcggcaac catgccggcg acggcgtcgg gatccgaaac gtctgccgca gccgggaatc
     4681 cgcctgtggc ggcgaccacc ccatccattc tgtgatcgcg gacccgtccg ttgactccga
     4741 cggccgcgcc ctcgtcggcc agcctctcgg cgatggccgc gccgatgccc tgtgtcgcgc
     4801 cggtgaccaa ggcgatgcgt ccgcgatggt gtccttcacg catcagatca ccgcgccgct
     4861 gttcggcgac agcacctcgc cggtgcagaa ccccgcgtcg tcactgacca gatagcgcac
     4921 cacttctgcg atctcgccgg gccgcgccag ccgccgtgca ggcagcgtcg ccaggtattc
     4981 cggtgcgcgc catggtgaat cgggggtgag cagcggagtg tcggtagggc cgggagcgac
     5041 ggcgttcacc cggactccgt gcgcggcaac ctcggcggcg aggctgcgca ccagcccgat
     5101 gatggcgccc ttcgcggccg cgtagtgcgc ctcgccgtcg ccgccaccca cggccagctc
     5161 ggacgtgatg gccacgatgg agccgcggcg ccgctcgatc atcgatggca gcgcggcgcg
     5221 ggcgacattg cgaaggccgc ccaggtgtac ccgcagcatg cggtgcagtt gcccatgggt
     5281 gatgtcggcg atcggcgtct cctcgtaata tcccgccgca ctgaccaccg cgtcgacagg
     5341 acccagctcg gtggccaccc ggcgcatcgt cacctcgacg gcggcggggt cggcgacgtc
     5401 cacggaatag ggagcggact cgtcgaggtc aaatcccgca acgcgatagc cgtcctgctc
     5461 cagcgccgcg gcgatggcgg caccgatgcc cgatgacgct ccggtgacga ccgcgactcc
     5521 gctcactgcg ggggcggttt gtcggcgaag cgttcggcga tcggcgcctg gatattgcgg
     5581 cgcactgaca ggtagatcgc cgccccgatc agccccagca ccaccgtggt gagcacgctg
     5641 gcccaactga tgtaccaggg cacgtccggc gaagtgcgcg gccacgcgac gttcaccgtc
     5701 tcgaagatga tccatagtga cgcgatgtag gtgatcgggg cgccccaccg gccgagggtg
     5761 aacgggccgg gttgccactt gccggacagg cggctgatgg cggcaccgat caccggaacg
     5821 ccgaacgcga tgtagaaccc gatgatgttg aagttgacca gaatgttgta gaactgtgtg
     5881 cccgccaaca gcacgaagac gattgctgcg acggcggtga gcgcaatcgc gttgaccggc
     5941 aggcgttccg ggcccgccag tccaccgagc accttcgagc ccggcaggct gcgatcgcgc
     6001 gccgcacccc agatgcaccg ggataccgcc gcctgcacgg caaggaagct cgacacgaat
     6061 ccgatcacga acatggcgag tagcggtttg gtcacgccct cgccgagatg cgtcgtgagg
     6121 gtgtcggaca ccacgtcgcc ggactgcgcg gcgacgacac cgggcaggtt gggaatcgaa
     6181 aggataagcg ccgcagcgga gaacatcacc acgagcccga cgaggagcag cgcaaggatg
     6241 atcgccttgg gcacattgcg ttccggctcg tcgacctcct cggcgaccga tccggccgcc
     6301 tcgaacccga ggaaggacca gccgacatag gcgatggcca gcagcatcgg gccggacgcc
     6361 cacgacgcgg cgtcgggaat acccgcgctg gagaacagca ccgagaacgg gttctcccgg
     6421 tagaacagca gcagcaccac gcccaggccg agcgagccga cgacctcgca ggtgatgctg
     6481 atggccacca tgatcttcaa gactctccgg cccaccatgt tcaccgcgga gccgacaagg
     6541 acgatggcga tcgcgaccga cgcgatctgc cagcggttgg gttcgccgac gccgaagatg
     6601 ttgagcagga agccggcggc tgcatacgac agcgtgccga gcgcgagcgt cagcccccac
     6661 atgtaggccc atgcggccac ccagccccac gtggtgccct ggacgtgacg cgaccactgg
     6721 tagacgctgc cggcatatgg ccagcgcgat gccagctcgc cgaacaccgc cgccaccatc
     6781 agctgtccga tgagcacgat cgggaatgcc cagaagaact tcggccccgc ggcgaacagc
     6841 cccagcgtga agatcgcgta gaccgcggcg atcggcgaca cgtcggcgaa ggccagcgac
     6901 agcgccgagc gaaagctgaa gccacgctgc agccgttccg gagcgggttc ggaaacggtg
     6961 ttggtgtcga tcgattcggc catgtcggct cccgtcgctg gaggtctcac caggggcaac
     7021 tgtcgtcccg gcggtcggca aaacggaata cgcccaacat ctgaacttga tgcgttgact
     7081 catacgatca tctagggtcg agccgtgtcg ggaaggaatc ggatggcgag gcgcacgccc
     7141 tgatggccgg gaactgccgc tttaggccag ccgccgtcgc aaggcagcgg tgtaaccacg
     7201 tgtttcgaac aggtgactga tggcgtatcg ggtccgcgac ctcctggaaa caccccacct
     7261 tcggctgacc ctgaaaggcg gcagccaagg cctggacaag ccggtgacct gggcgcagac
     7321 gtccgacctc caagagccgt ggtcgtatct
//
LOCUS       DASYLX010000333         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2975988,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000333 DASYLX010000000
VERSION     DASYLX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975988"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGP27_08145"
     CDS             <1..262
                     /locus_tag="VGP27_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851320.1"
                     /translation="VMALTRCSAIEAVEHDVRINAVSPSIARHKFLEKTSSADLLDRL
                     SEGEAFGRAAEPWEVAATIAFLASDYSSYLTGEVISVSSQRA"
     gene            259..867
                     /gene="kstR2"
                     /locus_tag="VGP27_08150"
     CDS             259..867
                     /gene="kstR2"
                     /locus_tag="VGP27_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator KstR2"
                     /protein_id="HEV7851321.1"
                     /translation="MTAQPGPQSASRRDELLQLAATMFAERGLRATTVRDIADSAGIL
                     SGSLYHHFKSKEQMVEEVLRDFLDWLFGRYREIVDTEPDPLARLKGLFMASFEAIEHR
                     HAQVVIYQDEAKRLSALPQFDFVEARNKEQRKMWVDVLNQGVSEGCLRPDLDVDLVYR
                     FIRDTTWVSVRWYQPGGPLTAEQVGRHYLAIVLGGITREGLK"
     gene            864..2021
                     /locus_tag="VGP27_08155"
     CDS             864..2021
                     /locus_tag="VGP27_08155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7851322.1"
                     /translation="MTPSSPAYVVEAVRTAVGKRNGSLAGVHPVDLGAAAWRGLFDRV
                     DADPGAVDDVIAGCVDAIGPQAGNIARLSWLAAGYPEEVPGVTVDRQCGSSQQAISFG
                     AQAIMSGTADLIVAGGMQNMSQIPISSAMTVAEQFGFTSPTNESKSWLQRYGDQEISQ
                     FRGSEMIAEKWNISREEMEQYSLTSHQRAQEAIRAGYFENEIIAVDGFVTDEGPRETS
                     LEKMAGLKTLVEGGRLTAAMASQISDGASAVLIASEQAVKDHGLKPRARIHHVSARGA
                     DPVYMLTGPIPATHYALEKTGLSIEDIDTVEINEAFAPVVMAWLQETKADPARVNPSG
                     GAIALGHPLGATGAKLFATMLNTLERTGGRYGLQTMCEGGGTANVTIIERL"
BASE COUNT          391 a          664 c          721 g          359 t
ORIGIN      
        1 cgtaatggcg ctcacccgtt gcagcgcaat cgaagccgtt gagcacgacg tgcgcatcaa
       61 cgcggtgtcg ccgagtatcg cgcgacacaa gttcctggag aagacgagtt cggccgatct
      121 gctcgaccga ctgtccgagg gtgaggcttt cggccgggcg gccgaaccat gggaggtcgc
      181 ggccaccatc gcgttcctgg cgagtgacta ctccagttac ctgaccggag aagtcatttc
      241 ggtatcgagc cagcgagcat gactgcgcag ccgggcccgc agtcggcgag cagacgcgat
      301 gagcttctgc agctcgccgc gacgatgttc gccgagcgcg gcctgcgcgc gacgacggtg
      361 cgagacatcg cggactcggc gggcatactg tctggcagcc tctaccacca tttcaagagc
      421 aaagagcaga tggtcgagga agtgctgcgc gatttcctcg actggctgtt cggtcggtat
      481 cgggaaatcg ttgacaccga gccggatccg ctcgcgcggc tcaaggggtt gttcatggca
      541 tccttcgagg ccatcgagca ccggcacgcc caggtcgtca tttaccagga cgaggccaaa
      601 cggctttcgg cgctgccgca gttcgacttc gtcgaagccc gcaacaaaga gcaacgcaag
      661 atgtgggtgg acgtgctcaa ccagggcgtc tcggagggct gcctgcgccc cgatctcgac
      721 gtcgatctgg tctaccggtt catccgcgac accacgtggg tttcagttcg ctggtatcag
      781 cccggcggac cgttgacggc cgagcaggtc ggcaggcatt acctggccat cgtccttggc
      841 ggcattacta gagagggatt gaaatgactc cgtcttcacc ggcatatgta gtcgaagccg
      901 tacgcaccgc ggtcggcaag cgaaacggat cacttgcggg tgtgcatccg gtggatctgg
      961 gggccgccgc gtggcgcgga ctgttcgatc gggtcgacgc cgatcccggc gcggtcgacg
     1021 atgtgatcgc gggctgcgtg gacgcgatcg gaccgcaggc gggcaacatc gcgcggctgt
     1081 cctggctggc cgccggctat ccggaagaag ttcccggcgt caccgtcgac cggcagtgtg
     1141 gttccagcca gcaggctatt tcctttggcg ctcaagcgat catgtccggc accgccgatc
     1201 tgatcgttgc aggcggcatg cagaacatga gtcagatccc gatctcgtcg gcgatgacgg
     1261 tggccgagca gttcggcttc acctcgccca ccaacgagtc gaagagctgg ctgcagcgct
     1321 acggcgatca ggagatctcg cagttccgag gctcggagat gatcgccgag aagtggaaca
     1381 tctcgcgtga ggagatggag cagtactcgc tgaccagtca ccagcgggcg caggaagcca
     1441 tccgcgcggg gtacttcgag aacgagatca tcgcggtgga cggattcgtc accgacgagg
     1501 gcccccggga gacctcgctg gagaagatgg ccgggctgaa gacgctcgtc gagggcggtc
     1561 gactgacggc ggcgatggcc agtcagatct ccgatggtgc cagcgcggtg ctgatcgcgt
     1621 ccgagcaggc cgtcaaggac cacggcttga agccgcgggc ccgcattcac cacgtcagcg
     1681 cccgcggtgc cgacccggtg tacatgctga ccggccccat cccggcgacg cattacgcgc
     1741 tggagaagac ggggctctcg atcgaggaca tcgacaccgt cgagatcaac gaggccttcg
     1801 cgcccgtcgt gatggcctgg cttcaggaga ccaaggccga tcccgcgagg gtcaacccca
     1861 gtggcggtgc cattgcgctc ggacacccgt tgggagcaac gggcgcaaag ctcttcgcga
     1921 cgatgctgaa cacgctggag cgcaccggcg gccgctacgg actgcagacg atgtgcgagg
     1981 gcggcggcac cgccaacgtc accatcatcg agcgcctcta gctccctgtt caccgccgag
     2041 tgtgtgggtt ctgagtgcat cgcccgagtg gcgtgcgaag gagcgtcaca ctcggcgagc
     2101 gtcacaacgg gcaacccgcc gcgcgtagct tgtcg
//
LOCUS       DASYLX010000334         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2756443,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000334 DASYLX010000000
VERSION     DASYLX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756443"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGP27_08160"
     CDS             <1..429
                     /locus_tag="VGP27_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEV7851323.1"
                     /translation="PHILTLSPPVRLEPEWLKPTAYMMAKYGMSLCALALAEELRGEG
                     IASNTLWPRTLVATAAVQNLLGGDEAMGRARKPDVYADAAYAVLNKPSREYTGNALLC
                     EDVLLESGVTDLSVYDCVPGSDLGVDLWVDTPNPPGYQAP"
     gene            complement(426..1589)
                     /locus_tag="VGP27_08165"
     CDS             complement(426..1589)
                     /locus_tag="VGP27_08165"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEV7851324.1"
                     /translation="MKCRGAVLRGIGCDWEVQEITLDPPREGEVLVKMAVAGVCHSDN
                     HYSTGDGVFSAGLAAMVEATGGTVPELFPMLGGHEGAGIVEDVGPGVRSVKPGDHVTF
                     SFIPACGSCRWCVTGMTYLCDNGAMMFDKGMVTDGTARRHVGGEDLTAMTQLGTFAEY
                     AVLAEESVIKIDESIPLEAASLVSCGVTTGWGSATVGVGTEPGDTVVIIGTGGVGINA
                     VQGARAAGARAIVAVDPVEFKRDSAKFFGATETVASAEEAIPMVQEMTAGVMADRVVL
                     TPSVLPAELLAPAMMLTRKGGTCLMTAIPKFDVNIVPLVLIDFIQSCKTLKGLLYGGM
                     NPRASMPMLLSLYRNGNLKLDELITRRYRLDQINDAFADMREGRNIRGIIEFD"
     gene            complement(1586..>3005)
                     /locus_tag="VGP27_08170"
     CDS             complement(1586..>3005)
                     /locus_tag="VGP27_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7851325.1"
                     /translation="RCQPEWEAMGPKGRRPWLMKFQEWIFDNAEHITDVVQSETGKPR
                     AEAATEAPASADSLNYWARNAERFLADKRARPHTPLQLVKRLTTVYRPYPLVGLIAPW
                     NFPFAMAGMDVPPALAAGAAVLLKPSEVTPLSAVEFVRGWREVGAPAVLGIATGAGGT
                     GAAVLANADYVQFTGSTATGRKVAMGCAERLIPYSLELGGKDPAIVLADADIERAVNG
                     IAWGGMFNSGQACVGVERVYVEAPLYDEFVERLTEKVASLRQGHDDRRYQFDVGAMAT
                     EAQCDLVARHVDDAVAAGARVTTGGKPTGVGTFFAPTVLTDVDHTMLCMTAETFGPTI
                     PVVKVADEEEAIRLANDSDYGLSATVWTSDYARGERIARRLDTGGVNINDVMSNLFSF
                     ALPMAGWKNSGVGARSGGAAGLLKYCRPQAITTPRLPAQANEVMWYPYSRRKGRLAMG
                     IMRVAAAHGLRRFGITRKGGAR"
BASE COUNT          495 a         1049 c          939 g          522 t
ORIGIN      
        1 ccgcacatcc tcaccctgtc accgccggtg cggctcgagc ccgagtggct caagcccacc
       61 gcttacatga tggccaagta cggaatgtcg ttgtgcgcct tggcattggc cgaggaactg
      121 cgcggcgagg gcatcgcatc caatacactc tggccgcgca cgctggtggc caccgcagcg
      181 gtgcagaacc tgctcggcgg cgacgaggcg atgggtcgcg cccgcaagcc cgacgtgtac
      241 gccgacgccg cgtacgcggt gctgaacaag ccgtcgcgcg agtacaccgg caacgcactg
      301 ttgtgtgagg acgtgctact cgaatccggc gtcaccgatc tgtcggtgta tgactgcgta
      361 cccggctcgg acctgggcgt cgacctctgg gtcgacaccc cgaatccgcc gggttaccaa
      421 gcgccttaat cgaattcgat gatgccgcgg atgttgcgcc cttcgcgcat gtcggcgaag
      481 gcgtcgttga tctgatcgag ccggtagcgc ctcgtgatca actcgtcgag cttgaggttg
      541 ccgttgcggt acagggacaa cagcatcggc atgctcgccc gcgggttcat tccgccgtac
      601 agaagcccct tgagggtctt gcacgactgg atgaagtcga tcagcaccag cggcacgatg
      661 ttcacgtcga acttcggtat cgccgtcatc agacacgtac ctcctttgcg ggtcaacatc
      721 atggccggcg caagcaattc ggccggtagc acgctcgggg tgagcaccac ccggtcggcc
      781 atcacgcctg ccgtcatctc ctgcaccatc gggatggcct cttcggccga cgcgacggtc
      841 tcggttgccc cgaagaactt cgctgaatcc cgcttgaatt caacgggatc gaccgcgacg
      901 atggccctgg caccggccgc ccgggcaccc tgcacggcgt tgatgcccac gccgccggtg
      961 ccgatgatga ccactgtgtc gccaggctcg gtcccgacac ccaccgtcgc cgaaccccag
     1021 cccgtcgtca caccgcagga caccagcgac gcggcttcga gcggaatcga ctcatcgatc
     1081 ttgatcaccg attcctctgc aagaacggcg tattcggcga atgtgccgag ctgcgtcatc
     1141 gcggtcaggt cttcaccgcc gacgtgacgg cgcgcggtgc cgtcggtgac catgcccttg
     1201 tcgaacatca tggcgccgtt gtcgcacaga taggtcatcc ccgtgacaca ccagcggcaa
     1261 cttccgcagg ccgggatgaa cgaaaacgtg acatggtcac caggtttcac cgaccggacg
     1321 ccgggcccga catcttcgac gatgcccgcg ccttcatgtc cgccgagcat cgggaagagc
     1381 tccggaaccg tgccgccggt cgcctcgacc atcgcggcga ggccggcgga gaagacgccg
     1441 tcgccagtcg agtagtggtt gtcggagtgg cagaccccgg ccaccgccat cttcaccagc
     1501 acctcgcctt cccgcggcgg gtccagcgtg atctcctgaa cctcccaatc acagccgatg
     1561 ccgcgcagaa cggcgccccg gcacttcatc gggcaccacc cttgcgcgtg atgccgaatc
     1621 gccgcagtcc gtgggcggcg gccacccgca tgatgcccat ggcgaggcgg cccttgcgcc
     1681 gcgagtacgg gtaccacatc acctcattgg cctgcgcggg cagtcgcggt gtcgtaatcg
     1741 cttgcgggcg acaatatttc agcaggcccg cggctccacc gctacgcgct ccgacgcctg
     1801 agttcttcca ccctgccatc ggcagtgcga agctgaagag gttgctcatc acgtcgttga
     1861 tgttgacccc gcctgtgtcg agccgccgcg cgatccgttc accgcgggca tagtcgctcg
     1921 tccagaccgt tgccgaaagt ccgtagtccg agtcgttggc cagccggatc gcctcttcct
     1981 catcggccac cttcacgaca gggatcgtcg ggccgaatgt ctctgcggtc atgcacaaca
     2041 tcgtgtgatc gacatcggtg agcacggtcg gcgcaaagaa cgtgccgacc ccggtcggct
     2101 tgccaccggt ggtgacccgc gcgcccgcgg cgaccgcgtc gtcgacgtga cgggcgacca
     2161 ggtcacactg tgcttcggtg gccatcgcgc ccacgtcgaa ctggtaccgg cggtcgtcat
     2221 gcccctgcct caacgacgcc accttctcgg tgagcctctc gacgaattcg tcgtacagcg
     2281 gcgcttcgac gtacacgcgc tcgacgccga cgcacgcctg tcccgaattg aacatgccgc
     2341 cccaggcgat gccgttgaca gcgcgttcga tgtcggcgtc ggcgaggacg atcgcagggt
     2401 ccttaccgcc gagttcgagg ctgtacggga tcaggcgctc ggcgcagccc atcgcgacct
     2461 tgcgtccggt ggccgtcgat ccggtgaact gaacgtagtc ggcgttcgcg agcaccgctg
     2521 cgcccgttcc gccagcaccc gtcgcgatgc ccagcaccgc gggcgcaccg acctctcgcc
     2581 agccgcggac gaattcgact gcgctcaacg gagtgacctc ggacggcttc aagaggacgg
     2641 cggcgccggc ggcgagggcg ggcgggacgt ccatgcccgc catcgcgaac ggaaagttcc
     2701 acggcgcgat aagtccgacg agcgggtacg gacggtaaac cgtggtcagg cgcttgacca
     2761 gctgcagcgg cgtatgcggc ctggcgcgct tgtcggccag gaatctctcc gcgttgcggg
     2821 cccagtagtt caggctgtcg gcggaggcag gggcttccgt cgccgcctcc gctctcggct
     2881 tgccggtctc cgactgcacc acgtcggtga tgtgctcggc gttgtcgaag atccactcct
     2941 ggaacttcat cagccatggt cggcgacctt tgggccccat cgcttcccac tcgggttggc
     3001 accgc
//
LOCUS       DASYLX010000335         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_782614,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000335 DASYLX010000000
VERSION     DASYLX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782614"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGP27_08175"
     CDS             complement(<1..513)
                     /locus_tag="VGP27_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7851326.1"
                     /translation="MTNSTALNASRRARELAELADLCLPCSSRARRPLDVVVIGGGIT
                     GTGIALDAASRGLTVALVEKHDLAFGTSRWSSKLVHGGLRYLATGNIGIARRSAIERG
                     ILMSRNAPHLVSAMPQLVPLLPAMGTPQRALVRTGFLAGDALRCMAGTRSSTLPRSRR
                     VSAGRAIDMVP"
     gene            complement(510..1091)
                     /locus_tag="VGP27_08180"
     CDS             complement(510..1091)
                     /locus_tag="VGP27_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7851327.1"
                     /translation="MLSISNDTPPDLGDRILDAAASCVLAYGVDRVTLAEIARRAGVS
                     RPTIYRRWPDTRALLAALLTSRIVGVLRGVPTRGSGREALVERIVGVAERLRHDDVVM
                     SVLHSAPELAMVYIAERLGTSQQILIDAVAAELTAAQRDGSVRAGDPRQLAAMCLLIT
                     QSTIQSARIVEPILDADTLAVELAHSLNGYLKP"
     gene            1112..>1527
                     /locus_tag="VGP27_08185"
     CDS             1112..>1527
                     /locus_tag="VGP27_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV7851328.1"
                     /translation="MKWDAWGDPDKAKPLSDGIRSLLTQALGTEASTLPELTADQVKL
                     RPSALSPADREALADVVGADNCGVDDHVRLLRAGGKSTLDLLRRRNTGIQDAPDAVLL
                     PGDDDEVAEILRICGQRSIAVVPFGGGTSVVGGVDP"
BASE COUNT          249 a          502 c          534 g          242 t
ORIGIN      
        1 gggcaccatg tcgatcgcgc ggcccgcgga gacccgtcgt gaccgcggca gcgtcgagga
       61 ccgggtgcct gccatgcagc gcagcgcgtc gccggcaagg aacccggtgc ggacaagcgc
      121 ccgttgcggg gtgcccatgg ccggaagcag tggcacaagc tgcggcatgg cgctcacgag
      181 gtgaggggcg ttacggctca tcaggattcc gcgttcgatc gcgctgcgcc tggcgatccc
      241 gatgttgccg gtcgccaggt agcgaaggcc gccgtgcacg agcttggagc tccaccggct
      301 ggtcccgaat gccagatcgt gcttctccac caacgccacc gtcagcccgc gcgatgcggc
      361 gtccaaagcg attccggttc cggtgatgcc gccgccgatg acgacgacat cgagcgggcg
      421 gcgcgcacgc gaggagcaag gcaggcacaa gtcggccagc tccgccaatt cgcgagcgcg
      481 ccgagatgcg ttgagcgcgg ttgagttcgt cacggcttga ggtatccgtt cagtgagtgg
      541 gcgagttcga cggcgagtgt gtcggcgtcg aggatgggtt cgacgatccg tgcggactgg
      601 atcgtcgact gtgtgatcag cagacacatg gcggcgagtt ggcgcgggtc gcccgcgcgc
      661 acgcttccgt cgcgttgggc cgccgtcagc tcggcggcca ccgcgtcgat gaggatctgc
      721 tggctggtgc cgagccgttc ggcgatgtac accatggcca gttccggtgc ggagtgcagc
      781 accgacatga cgacgtcgtc gtggcgaagc cgttcggcaa cgccgacgat ccgctcgacc
      841 agtgcttcgc gtccgctgcc gcgtgtcggc acaccgcgca gcacgccgac gatccgcgag
      901 gtcaacagtg cggccagcag cgcgcgggtg tctggccagc ggcggtagat cgtcggcctg
      961 ctcaccccgg cccggcgggc gatctcggcg agggtcaccc ggtccacccc gtaagccagc
     1021 acgcagctcg ccgccgcatc caggatccga tcgccgaggt ccggcggtgt gtcgttactg
     1081 attgacaaca tatgtaatac tgtaacgcat gatgaagtgg gatgcatggg gtgacccgga
     1141 taaagccaag ccgttgtccg acggaattcg gagtctgctg acgcaggcac ttggcaccga
     1201 ggcctcgacg cttcccgaat tgaccgccga ccaggtcaaa ctgcggccgt cggcgctctc
     1261 gcctgccgac cgcgaagcgt tggccgacgt cgtcggcgca gacaattgcg gcgtcgacga
     1321 ccacgtcagg ctgcttcgcg cgggcggcaa atcaactctc gatctgctac gacgccgcaa
     1381 caccgggatt caggacgcac ccgacgccgt actgctgccc ggtgacgacg acgaggtggc
     1441 cgagattctg cgcatctgcg ggcagcgaag catcgccgtc gtccccttcg gcggtggcac
     1501 cagcgtcgtc ggcggtgtcg acccgca
//
LOCUS       DASYLX010000336         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2646727,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000336 DASYLX010000000
VERSION     DASYLX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646727"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGP27_08190"
     CDS             <1..190
                     /locus_tag="VGP27_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7851329.1"
                     /translation="LPVDEWMGVQADSHWAADGISVGTATMFDRIGAFGSALVTAIAN
                     PAAQIDFSDDPFPSRSPS"
     gene            complement(194..610)
                     /locus_tag="VGP27_08195"
     CDS             complement(194..610)
                     /locus_tag="VGP27_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851330.1"
                     /translation="MRNKAVGVGVIAAAAASTMTMMATPGIAAADDYAGQKYSDVTSK
                     LADASLTGVIATRTGDTVNDDDCVVTSSEKAPWVKGDDFSPVTDTVLLNLNCYSGVAS
                     ATTAGNSKASPAGKAAIAAAQQEEQQAAADQAKAKQ"
     gene            complement(723..1196)
                     /locus_tag="VGP27_08200"
     CDS             complement(723..1196)
                     /locus_tag="VGP27_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV7851331.1"
                     /translation="MRVESTANRRGLADRFHRCCAAVVGRLPFGMNSVVAPTFLGFTL
                     INGFTFGIDLLLLSGLRAGLGLPLPAAVTVAYACTFALSYHLNRSMNFQSHAPVGPQL
                     ASYVLVVVVNYLAFILGVSSALTALGLDFRLARILAGACEAVYMYSAMRWVVFRR"
     gene            1383..4148
                     /locus_tag="VGP27_08205"
     CDS             1383..4148
                     /locus_tag="VGP27_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7851332.1"
                     /translation="MPSRVVSRPEEGRAVAAFLDAAASRPAGLVAEGEPGIGKTTLCL
                     SAIELAEQRGFQVLSARPAQAESVLAYASLADLLSGVDASVLTSLPAPQRLAIDRVLL
                     RADAEDLTTDQRAVGAGFLSVVELLAEQAPVLVAIDDLQWLDPSSRNVMAFATRRLSS
                     RVGVLVTVRSDPDGGVSGASWLQLPQPDGVQRVQIRPLSLGGLNAVITERLGRSLSRP
                     AIVRIQEVSQGNPFYALELARAMDGDSRSAEMPLPMTLAELVRARIGSLDDSTHAVLL
                     AMACTAVPTVELVSRATGLKAGDVVAQLDDVEAKGIVAIDGHRVRFAHPLLARGLYTD
                     VTPARRRSMHRRLAEIVDEPELKARHLAMAATHGDELTLITLDEAADMARSRGAPAAA
                     ADLLDLAIGLGGGTPERRILSAAHHFDAGEPAKARGLLQETIERLEPGPRRAEAYTLL
                     ALVASLNDSFDEAADLLEQALDDVGDELAQRVAILVSLSFALVNAGRMDPAVRHIEEA
                     VTSATELGDPHALSQALGMRMMLHFMRGDGIDERSLRRAVELEDHDADVSSAFSPRVQ
                     RALMLAWTGELDRAHQEVRTIRRRCIDRGEEGELSFIDFHSVLIDIWRGSMADAMLIA
                     EDAVERAHQLNGDLPLSVALTARAAVAAYAGDEDTARRNVAEARAASERCGSRRLGEW
                     PVTMLGFLELSLGNYEAVVNTVAPLMPMLTAVPDSTEIINASYVPDAAEALIHLGRLA
                     EAVSMVELLERNGQRLDRPWMKAVGARCRAMELAARGDVDAANRAVEQALSEHDRLPM
                     PFERARTQLLAGQLQRRQRQKDQAATTLREALKTFEDLNTPLWADRARAELARADVGT
                     RRMSSLSPSEQRVAELAASGMTNRDAAAALFISPKTVESNLARIYRKLGIHSRAELGR
                     HMGQPVE"
     gene            complement(4157..5248)
                     /locus_tag="VGP27_08210"
     CDS             complement(4157..5248)
                     /locus_tag="VGP27_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851333.1"
                     /translation="MKSHLNLAPLGAFAIVCITATACGSHTVSSPGPNSSAAASDTAG
                     ASGAPATGGDMPDSAQPWTADVSGVAKSDRSDAILRALTDAGGWGNGNVLQTDFSISI
                     FNADANTPRMDVVGIENYCGGGPDCDSVPAQMPVPEDATIEGSQDLSCDISGSTEGQG
                     DCHLLVADRDEQKLYELYQANQTGEGMTTQAMFVWDMNKSYPDNLRGDQCTSADAAGF
                     PIAAMTPTADEVASGAVNHALRFILPNDRMKAGVYVHPATHAGGPESADSDAPPYGVR
                     FRLKPDFDESSYNDSERVIIKALKAHGMLLADGGDIALTFADDRNNTAKWADLGIDAQ
                     SFSGITVGQFEVVELGDEIPLTYDCVRNP"
     gene            complement(5347..>5581)
                     /locus_tag="VGP27_08215"
     CDS             complement(5347..>5581)
                     /locus_tag="VGP27_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851334.1"
                     /translation="RYPDPDTFNIHRDNISHLTFGKGVHYCLGANLARLEGRVALDEL
                     LNRWPEWGIDYDSAVPAPTSTVRGWERLRMVLP"
BASE COUNT          917 a         1847 c         1869 g          948 t
ORIGIN      
        1 gttgcccgtc gacgaatgga tgggcgtgca ggccgactcg cactgggcgg ccgacgggat
       61 ctcggtcggg acggcgacga tgttcgaccg catcggtgcg ttcggctcgg cgttggtgac
      121 cgcgatcgcc aacccggctg cccagatcga cttcagcgac gatccgttcc cgtcacgcag
      181 cccgtcctga cgcctattgc ttggctttcg cctggtccgc ggccgcctgt tgctcctctt
      241 gctgggccgc agcgatggcc gccttgcccg cggggctggc cttcgagtta cccgccgtcg
      301 tcgccgacgc cacacccgag tagcagttca ggttcaatag caccgtgtcg gtgaccggcg
      361 agaagtcgtc gcccttgacc cacggcgcct tctccgagct cgtgacgacg cagtcgtcgt
      421 cgttcacggt gtcgccggtc cgcgttgcga tgacgccggt gaggctcgca tcggcgagtt
      481 tcgatgtgac gtcggagtac ttctgtcccg cgtagtcgtc tgcggcggcg atgccggggg
      541 tcgccatcat cgtcatcgtc gacgccgccg ccgcggcgat gacacctaca ccaacggcct
      601 tgtttctcat cacgtccaac ctcctatgag ttgggcaaac tctaaaaacc tgatctggca
      661 ggaccctggg ctccggcgat gcaaacactg ccgatttgct tcgcggtcgc tgagcgtgcg
      721 tgtcagcggc ggaagacgac ccagcgcatg gcgctgtaca tgtagaccgc ctcacaggcc
      781 cctgccagga tcctggccag gcggaagtcc agaccgaggg cggtcagggc actcgacacg
      841 ccgagtatga acgccaggta gttgacgacg accaccagca cataactcgc taactgcggg
      901 ccgaccggtg cgtgcgactg gaagttcatg gagcggttca ggtggtagct cagggcgaac
      961 gtgcacgcgt aggccacggt taccgccgcc ggtagcggta agcccagccc tgcacgcaga
     1021 cccgacagca gcaagaggtc gataccgaac gtgaacccgt tgatcaacgt gaagcccaag
     1081 aaggtcggcg ccacaacaga attcattccg aacggtagac gcccaaccac tgctgcgcag
     1141 caccggtgaa agcggtcggc cagacctctc cggttcgccg tcgactcgac ccgcacgtga
     1201 ccacgctgcc atggtcacgt atccacgacg tgagcagtgg gccaatttcg gaccacacga
     1261 cgtgacagcc ggggtgaaac atgctgctcg gcaacatcgc tcggaggctt cgttatctgg
     1321 atagtggcgg caatatgccc atctgatgcg catccagcaa aatatgttgc gatactaagc
     1381 aggtgcccag ccgcgtcgtc agccgaccag aagagggtcg agcggtcgcg gcgtttctcg
     1441 acgctgcggc gtcccggccg gccggcttgg tcgccgaagg tgaacccggt atcgggaaga
     1501 cgacactgtg cttgtccgcg atcgaactcg ccgaacagcg cgggttccag gtgttgtcgg
     1561 cgcggccggc gcaggccgag tccgtgctcg cgtacgcctc actggcggac ctgctttcgg
     1621 gcgtcgatgc gtcggtgctg acgagcctgc cggccccgca gcggctggcg atcgatcgag
     1681 tccttctgcg cgccgacgcc gaggatctca ccaccgacca gcgcgccgtc ggggcgggct
     1741 tcctgtcggt cgtcgaactc ctcgcggagc aggcccctgt gctggtggcg atcgacgacc
     1801 tgcaatggct cgacccgtcg agccgaaatg tgatggcatt cgcgacgcgc cgactctcgt
     1861 ccagggtcgg cgtgctggtc acggtccgtt ccgatcccga cgggggcgtc agcggtgcct
     1921 cctggttaca gctgccgcag ccggacggcg tgcagcgggt gcagatccgg ccgttgagcc
     1981 tcggcgggtt gaacgccgtc atcaccgagc ggctcggcag atccctgtct cggccggcaa
     2041 tcgttcgaat acaagaggtt tcgcagggaa acccgttcta cgcgctcgaa ctggcgcgtg
     2101 cgatggacgg tgacagcaga agcgcggaga tgccgttgcc gatgacgctc gccgagttgg
     2161 tgcgcgcgcg gatcggcagc ctcgacgact ccacccacgc tgtgctgctg gcaatggcct
     2221 gcaccgccgt gccgaccgtt gagctggttt cccgggcaac cggtttgaag gccggcgacg
     2281 tcgttgcgca actcgacgat gtcgaggcga agggcatcgt cgcgatcgac ggtcatcgcg
     2341 tccggttcgc gcatccgctg ttggccaggg ggctctacac cgatgtgacc ccggcgcggc
     2401 gcaggtccat gcaccgcagg ctggcggaga tcgtcgacga acccgagctg aaggcaagac
     2461 atctcgcgat ggcggccacc cacggcgacg agctgacgct gatcaccctc gacgaggccg
     2521 cggacatggc ccgcagcagg ggcgcacccg ccgcggcggc cgacctgctc gacttggcca
     2581 tcggcctcgg aggcggcacc cccgagcggc gaatcctctc ggcggcacac catttcgacg
     2641 cgggcgagcc cgccaaggcc cgcggcctcc tgcaggagac catcgaacgt ctcgagccgg
     2701 gtccaaggcg ggcggaggcg tatacgctgc tggcccttgt ggcttcgctc aacgacagct
     2761 tcgacgaggc cgccgacctg ctcgagcaag ctctcgatga cgtgggcgat gagctcgccc
     2821 agcgggtcgc gatcctggtg tcgctgtcgt tcgccttggt caacgcgggg cgcatggatc
     2881 cggcggtgcg gcacatcgaa gaggccgtga caagcgccac cgagctcggc gacccacacg
     2941 ccctgagtca ggcgctgggc atgcggatga tgctgcactt catgcgcggc gacggcatcg
     3001 acgagcgcag cctccgacgg gccgtcgagt tggaagacca tgacgccgat gtgtcgtcgg
     3061 ccttcagtcc ccgcgtccag cgcgccctaa tgctcgcctg gaccggggag ctggaccggg
     3121 cgcaccagga ggtgcgaaca atccgccgcc gatgtatcga ccgtggcgag gaaggcgagc
     3181 tgtcgttcat cgacttccat tcggtgctga tcgacatctg gcgcggttcc atggccgacg
     3241 ccatgctgat cgccgaggat gccgtcgagc gggcgcatca gctcaacggt gacctgccgt
     3301 tgtccgtcgc gctgacggcg cgcgccgccg tcgccgcata cgccggggat gaggacaccg
     3361 cgcgccgcaa cgtggccgag gcgcgtgccg ccagcgaacg ctgtggatcg cgcaggctcg
     3421 gcgaatggcc ggtgacaatg ctcggcttcc tcgagctctc actgggaaac tacgaagcgg
     3481 tggtcaatac cgttgcgccc ctgatgccga tgctgacggc cgtgccggac tcgaccgaga
     3541 tcatcaacgc gtcctatgtc cctgacgccg ccgaggcgct catccacctt ggccggctgg
     3601 ctgaggcggt gtcgatggtc gagctgctcg aacgcaacgg gcaacggctc gaccgaccgt
     3661 ggatgaaagc cgtcggcgca cgctgtcgag ccatggagct ggcggcgcgg ggtgatgtcg
     3721 acgccgccaa ccgcgctgtc gagcaggcac tctccgaaca cgaccggctg cccatgccgt
     3781 tcgaacgtgc gcggacccag ctgctggccg ggcagcttca gcgcaggcag cgtcaaaagg
     3841 accaggccgc aacgaccctg cgcgaggcac tgaagacatt cgaggacctg aacacccccc
     3901 tgtgggccga ccgagcccgc gccgaactcg cccgtgccga tgtcggcacc cgccggatgt
     3961 ccagcctcag cccatccgag cagcgggtgg ccgaactcgc cgcctcaggc atgaccaacc
     4021 gtgatgccgc cgccgcactg ttcatcagcc cgaagaccgt tgagtcgaat ctggcccgca
     4081 tctaccgcaa gctgggcatc cattcacgcg ccgaactcgg caggcacatg ggccaacccg
     4141 tcgagtagga ccgccgtcac gggttgcgca cgcagtcgta ggtgagtggg atttcgtcac
     4201 ccaactcgac gacctcgaat tggccgacgg tgatgccgct gaatgactgc gcgtcgatgc
     4261 cgaggtcggc ccatttggcc gtgttgttgc ggtcgtcggc gaaggtcagc gcaatgtctc
     4321 cgccgtcggc cagcagcatg ccgtgggcct tgagcgcctt gatgatcacc ctctcggaat
     4381 cgttgtagct ggattcgtcg aagtcgggct tcaaccggaa ccgaacaccg tagggcgggg
     4441 cgtcggagtc ggcgctttcc gggccgccgg cgtgagtcgc cgggtgcacg tagacgccgg
     4501 ccttcatccg gtcgttcgga aggatgaatc gcagggcgtg attcaccgca ccggaggcga
     4561 cttcgtcggc ggtcggtgtc atggcggcga tcgggaaccc tgcggcgtct gcgctggtgc
     4621 actggtcgcc acgcagattg tccgggtacg acttgttcat gtcccagacg aacatcgcct
     4681 gtgtcgtcat gccctcgccg gtctggttgg cctggtagag ctcgtagagc ttctgctcgt
     4741 cgcgatccgc gaccagcaga tggcagtcgc cctggccctc ggtgctgccc gagatgtcgc
     4801 aggaaaggtc ttgcgaccct tcgatagtgg cgtcctcggg caccggcatc tgcgccggaa
     4861 cgctgtcgca gtccgggccg ccgccgcagt agttctcgat tcccaccacg tccatccggg
     4921 gagtgttggc gtcggcgttg aagatcgaga tcgagaaatc ggtctgcagg acgttgccgt
     4981 tgccccagcc ccctgcgtcg gtgagggccc gaagaatcgc atcactgcgg tcggacttcg
     5041 caactcccga cacgtcggcc gtccagggct gagcggaatc gggcatatcg ccgccggtcg
     5101 ccggggcgcc gctcgcaccg gcggtatccg aagccgccgc actcgagttg gggcccgggc
     5161 tcgagactgt gtgagatccg cacgccgtgg cggtgatgca gacgatggcg aaggcgccga
     5221 gaggagcgag gttcagatgc gatttcatgc agcagtcagc gcctctcctg atagtgagct
     5281 gtgcgccgac gctatcccgg acgagttcag gcagatatcg ggtgtttccc tacgttcggc
     5341 ctgcggtcac ggcagcacca tccgcagccg ctcccagccc cgcaccgtcg acgtcggcgc
     5401 aggtacggcg ctgtcgtagt cgattcccca ctcgggccac cggttcagca attcgtcgag
     5461 cgctacccgg ccctcgagcc gggcgaggtt ggccccgagg cagtagtgca cacccttgcc
     5521 gaacgtcaaa tggctgatgt tgtcacggtg gatgttgaag gtgtcggggt cggggtagcg
     5581 c
//
LOCUS       DASYLX010000337         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2428134,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000337 DASYLX010000000
VERSION     DASYLX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428134"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /gene="dnaE"
                     /locus_tag="VGP27_08220"
     CDS             complement(<1..1988)
                     /gene="dnaE"
                     /locus_tag="VGP27_08220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV7851335.1"
                     /translation="MLDGAAKIAPMLAEAQRLEMPAIGMTDHGNMFGASEFYNLATEA
                     GIKPIIGVEAYIAPGSRFDTRRILWGDPGQKADDVSGSGSYTHLTMVAENATGVRNLF
                     KLSSLASFEGQLGKWPRMDAEIVAEHAAGIIATTGCPSGEVQTRLRLGHDREALESAA
                     KWREIFGADNYFLELMDHGLSIERRVRDGLLEIGRKLDIPALATNDCHYVTRDAAHNH
                     EALLCVQTGKTLSDPNRFKFDGDGYYLKSAAEMRSLWDDDIPGACDSTLLIAERVQSY
                     ADVWATRDRMPVFPVPDGHDQASWLRHEVDAGLRRRFPAGVPPEYVDRAAFEIEVICG
                     KGFPSYFLIVADLVGYARSVDIRVGPGRGSAAGSLVAYSLRITDIDPIEHGLLFERFL
                     NPERASMPDIDIDFDDRRRGEMVRYAADKWGHDRVAQVITFGTIKTKAALKDSARIHY
                     GQPGFAIADRITKALPPPIMAKDIPLSGITDPSHERYKEAVEVRNLLDTDPDVRTIYE
                     TARGLEGLVRNAGVHACAVIMSSEPLIEAIPLWRRPQDGAIITGWDYPSCEAIGLLKM
                     DFLGLRNLTIIGDTIENIKANRGIDLDLDAVPLDDPATFDLLGRGDTLGVFQLDGGPM
                     RDLLRRMQPTDFEDVVAVIALYRPGPMGMNAHNDYADR"
     gene            complement(2151..>3317)
                     /locus_tag="VGP27_08225"
     CDS             complement(2151..>3317)
                     /locus_tag="VGP27_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV7851336.1"
                     /translation="YGRLLAVLAAPTRDVGTAEDALADALERALQRWPDDGIPANPEA
                     WLLTVARNRLRDVWKSHAYRMTDPLGETAEDAGEVDDGMPAIPDRRLELMLVCAHPAI
                     APNIRTPLMLQTVLGVEASAIAEAFAVEPVTMAQRLVRAKKRIRDAGIPFVLPERDDL
                     AARLPAVLEAVYGAYAIDWQWMPQAAPIETLAAEALHLALVLTELLPDEPEVLGLAAT
                     VCLSEARRSARRDDDGRYIPLDDQDAARWDAALIARGEAFLRRAHDFGRPGRFQYEAA
                     IQSAHCSRPTDPEPLRKLYRALMRVAPSLGAAVALAAVDAAIDGPDAGLRALDAIGDS
                     AVERFQPAWSTRAHLLAEAARAGPAAHAYRAAIELTTDRGVADYLREQLTSLTR"
BASE COUNT          506 a         1110 c         1158 g          543 t
ORIGIN      
        1 ttacggtcgg cgtaatcgtt gtgggcgttc atgcccatcg gcccgggccg gtacagcgcg
       61 atgacggcga cgacgtcctc gaagtccgtc ggctgcatgc ggcgcagcag gtcccgcatc
      121 gggccaccgt cgagctggaa gacgccgagc gtgtcgccac ggcccagcag gtcgaacgtc
      181 gccggatcat cgaggggaac cgcgtccaga tcgaggtcga taccccgatt ggccttgatg
      241 ttctcgatcg tgtcgccgat gatcgtcagg tttcgcagac cgaggaagtc catcttcagc
      301 aggccgatgg cctcgcacga cggatagtcc caaccggtga tgatcgcgcc gtcctgcggg
      361 cgcctccaga gcggaatggc ctcgatcagc ggctcggagc tcatgatcac cgcgcaggcg
      421 tgcacaccgg cgttgcgaac cagaccctcc agcccgcgcg ccgtctcgta gatggttcga
      481 acgtccgggt cggtgtcgag gagattgcga acctcgacgg cctccttgta ccgctcgtgg
      541 ctggggtcgg tgatgcccga gagcggaatg tccttggcca tgatgggcgg cggcagcgcc
      601 ttggtgatgc ggtccgcgat cgcgaatccc ggttggccgt agtggattcg ggccgaatcc
      661 ttcagtgcgg ccttcgtctt gatggtgccg aaggtgatga cctgggcaac gcggtcgtga
      721 ccccacttgt cggcggcgta gcgcaccatc tcaccgcgcc tgcggtcgtc gaagtcgatg
      781 tcgatatcgg gcatggatgc gcgttcgggg ttgaggaaac gctcgaacag caggccgtgc
      841 tcgatcgggt cgatgtcggt gatccgcagc gagtaggcga ccagcgagcc tgccgccgac
      901 ccgcggcccg gccccacccg gatgtccacc gaccgcgcgt agccgacgag gtcggcgacg
      961 atcaggaagt acgacggaaa ccccttgccg cagatgacct cgatctcgaa cgcggcgcgg
     1021 tcgacgtact cgggcggcac accggccggg aaccgccgcc tcaggcccgc gtccacctcg
     1081 tgccgcagcc atgacgcctg atcgtgccca tcgggcaccg ggaagaccgg catgcggtcg
     1141 cgcgtcgccc acacgtccgc gtatgactgc acgcgttcgg cgatgagcag cgtcgagtcg
     1201 caagcgccgg gaatgtcgtc gtcccacagc gaccgcatct cggcggccga tttcaggtag
     1261 tagccgtcgc cgtcgaactt gaaccggttg gggtccgaca gcgttttgcc ggtctgcacg
     1321 cacagcagcg cctcgtgatt gtgcgcggcg tcgcgggtga cgtagtggca gtcgttggtg
     1381 gccagggccg ggatgtcgag tttgcggccg atctccagca acccgtcgcg gacccgccgc
     1441 tcgatcgaaa ggccatggtc catcagctcg aggaagtagt tgtcagcgcc gaagatctcc
     1501 cgccatttcg cggctgactc cagcgcctcg cgatcatggc cgagccgcag ccgggtctgc
     1561 acctcccccg acgggcagcc ggtggtggcg atgatgcctg ccgcgtgctc ggcgacgatc
     1621 tcggcgtcca tcctgggcca cttgccgagc tggccctcga acgacgccag cgacgacagc
     1681 ttgaacaggt tgcgcacgcc cgtcgcgttc tccgcgacca tcgtcaggtg ggtgtaggag
     1741 ccactgcccg acacgtcgtc ggccttctgg cccgggtcgc cccacaggat gcggcgggtg
     1801 tcgaagcggg atccgggcgc gatgtaggcc tcgacgccga tgatcggctt gataccggcc
     1861 tcggtggcca agttgtagaa ctcgctggcg ccgaacatgt ttccgtggtc ggtcatgccg
     1921 atggccggca tctccagccg ctgggcctct gccagcatcg gagcgatctt cgcggcgccg
     1981 tccagcatcg aatattcggt gtggttgtgc aggtgcacga aggacgaccg atgtgggggc
     2041 acctcccgct cgcgggggac gctcacgcca agagcagatg gacccgagtt catagggacg
     2101 ccagtgtatg accgcccacc gacgagcacg ggcgtgtcgg agtgcgtgtc ttatcgcgtt
     2161 agcgaagtta gctgttcacg caggtagtca gcgacacctc gatcggtcgt cagctcgatg
     2221 gcggcgcggt atgcgtgtgc tgcgggcccg gcccgggccg cctcggccaa caagtgggcg
     2281 cgggtgctcc aagccggctg gaagcgctcg acggccgaat cgccgatggc gtcgagtgcg
     2341 cgcagcccgg cgtccggtcc gtcgatcgca gcgtcgacgg cggccagcgc gacggccgca
     2401 cccagcgacg gcgccacgcg catcagggcg cgatacagct ttcgaagcgg ctccgggtcg
     2461 gtcggtcgag agcagtgtgc cgactggatt gcggcctcgt actggaaccg gcccggccgg
     2521 ccgaagtcgt gagcgcgtcg caggaacgcc tcaccgcgcg cgatcagcgc ggcatcccag
     2581 cgcgccgcat cctggtcgtc gagcgggatg tatcggccgt cgtcatcgcg tcgggcggac
     2641 ctgcgggcct cagacaggca gaccgtcgcc gccagcccga gcacctccgg ctcgtcgggc
     2701 agcaactcgg tcagcaccag cgccaggtgc agcgcctccg cggccagcgt ttcgatcggc
     2761 gcggcctgag gcatccactg ccagtcgatc gcgtacgcgc cgtagaccgc ttcgagcacc
     2821 gcgggcagcc gtgcggccag gtcgtcgcgc tcgggcagca cgaacggaat tcccgcgtcg
     2881 cggatgcgct tcttggcccg caccagacgc tgcgccatgg tgacgggttc gaccgcgaag
     2941 gcctcggcga tggcgctcgc ctcgaccccg agcaccgtct gcagcatcaa cggcgtgcgg
     3001 atgttcggcg cgatggcggg gtgggcgcag accagcatca gctcgagccg ccggtcgggg
     3061 atggcgggca tgccgtcgtc gacttcgccg gcgtcttcgg cggtttcgcc gagcggatcg
     3121 gtcatccggt acgcatgtga cttccacacg tcgcgcaacc ggttgcgggc gaccgtcagc
     3181 agccacgctt cggggttggc gggaattccg tcgtccggcc agcgttgcaa cgcgcgttcc
     3241 agcgcatcgg ccagcgcgtc ctcggcggtt ccgacatctc gggtgggcgc cgccagcacg
     3301 gcgagcagcc gaccgta
//
LOCUS       DASYLX010000338         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2099579,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000338 DASYLX010000000
VERSION     DASYLX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099579"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGP27_08230"
     CDS             <1..241
                     /locus_tag="VGP27_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851337.1"
                     /translation="VPVFKSLTNTALPNAVRLVGEVLEGAAKPVGSDAEGVIELKDQQ
                     ESDTKVVKVAPKVTRRSAPKGTPRRAAPRRARVNT"
     gene            complement(532..1878)
                     /locus_tag="VGP27_08235"
     CDS             complement(532..1878)
                     /locus_tag="VGP27_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851338.1"
                     /translation="MSEQSQHQLTGVAAVAALADLGLASLAAGPIARRRTVVGLLERV
                     QGDHRALRRMQVLRRQFGSGPVELVIPGRRILVLLDPTDVGRVLEDTPSPFDPATWEK
                     RRALEQFQPHAVLISRGPIRQQRRQLNETALDTTAELHHLANRFADTIAEEAAKIAAD
                     TLSSGSIDSAQFTKAWWQLVRRLVLGDAARDDNAVTDDLWRLRRAGNWSFLALPHANR
                     RARFFDRLYRYAETAEPQSLIGTLAREPAGAATDPIGQVPHWLFAFDAAGMALLRCAA
                     LLADRPEELGRCETSNAEMMGVRPFLRACMLESVRLWPTTPILLRETTAETTWRDGPD
                     RFTVPYPATVLIPVPALHRDQKLSPFANDFVPDIWLDGRAQQYPQLVPFSAGPAECPG
                     RNLVLFVTSTLLAHLFDAMDLALESVPGLAAGRPLPLTLNQFGLTFKARARRSITG"
     gene            2039..2722
                     /locus_tag="VGP27_08240"
     CDS             2039..2722
                     /locus_tag="VGP27_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEV7851339.1"
                     /translation="MSQRRRIPPVLHNNGLLLACLGLFAVFFVGMVVSGTATYNQEQQ
                     EHGSQQQVSVVGYLTTGDFVEATFENWESEFLQMGMYVVLTAFLFQKGSSESKPLNES
                     APQDHDPRRTAVNQHTPWPVRKGGWVLRVYEHSLAIAFFVLFFASWALHAVGGDKAFN
                     EEQVQHGQTAISVWRYVTTSQFWFESMQNWQSEFIAVAAIVGLSIFLRQRGSAESKPV
                     AEPHGHTSA"
     gene            complement(3280..4014)
                     /locus_tag="VGP27_08245"
                     /pseudo
     CDS             complement(3280..4014)
                     /locus_tag="VGP27_08245"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4137..>4415)
                     /locus_tag="VGP27_08250"
     CDS             complement(4137..>4415)
                     /locus_tag="VGP27_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851340.1"
                     /translation="DTTTHPTMTSNVKFMNFYPFNDIETISPRPMLFIAGQNAHSLEF
                     SEDAYALAAEPKELVIIPGAGHVDLYDRVNLIPFDKLTGFFQNSLRNG"
BASE COUNT          789 a         1300 c         1474 g          852 t
ORIGIN      
        1 ggtacctgtc tttaagtcgt tgaccaacac cgccttacct aatgcagtgc gtctagtcgg
       61 tgaggtgctc gaaggcgccg ccaaaccggt gggtagcgac gccgagggag tcattgagct
      121 caaagatcag caggagtccg acacaaaagt tgtcaaggtg gcaccgaaag tcacccggcg
      181 gagcgcaccg aaaggcaccc cgcggcgagc ggcgcctcgc cgcgcacgcg taaataccta
      241 agtgcgctgt gcagcgaggt cctactccta gagcagcagc gctgagcact cttggtttgt
      301 gtccgtgatg ttttccgact gccagtgacg cattcaactg acgctcgact tgacccgagt
      361 cgcacggcga gggcagccgt acgtgaaacc aggcggtcgc ccacttgaag aactccaccc
      421 tgatttgccc tatggagttt cgctagacgg cctgacaggg acgcgtcgag tcgtggatgt
      481 ggtcctcgac ccgcgtggtt ttcgactggg atagagggga atcccgagtc gtcatcctgt
      541 aattgagcgt ctggcgcgcg ctttgaaggt caagccgaat tggttgagcg tcaacgggag
      601 cgggcgaccg gctgccaaac cgggcaccga ttcgagcgca aggtccattg cgtcgaacag
      661 gtgggctagc agcgtgctgg tgacgaagag gacgaggttg cggccggggc attcggccgg
      721 gcccgcgctg aacggcacca gctgcggata ctgctgggcg cggccgtcga gccagatgtc
      781 gggaacgaag tcgttggcga agggcgatag cttctgatcg cggtgaaggg ccggaaccgg
      841 gatcagcact gtcgctggat acggcacggt gaacctgtcg ggtccatccc gccaggtggt
      901 ttcggcagtg gtttcgcgca acagaatcgg tgtcgtcggc cataaccgca cggattccaa
      961 catgcacgca cgcaagaatg ggcggacacc catcatttcg gcattgcttg tctcacaacg
     1021 gcccaactct tcgggacgat ccgcgagaag ggccgcgcag cgcagtagag ccatgccggc
     1081 ggcgtcgaat gcgaagagcc aatgcggcac ctggccgatc gggtctgtgg ccgcgcctgc
     1141 cggttcgcgc gccagtgttc cgattaggct ttgtggctca gcggtttcgg cgtagcgata
     1201 aagcctgtcg aagaagcgcg cccgccggtt ggcgtgggga agggcgagaa acgaccagtt
     1261 ccctgccctt cggagccgcc acaaatcgtc ggtgactgca ttgtcgtcgc gggcggcgtc
     1321 gcccagcacc agacgtcgta ctaattgcca ccaggccttc gtgaactgcg ccgaatcgat
     1381 ggaacccgac gatagggtgt ccgccgcgat cttcgccgcc tcctcggcga tggtgtccgc
     1441 gaagcggttc gcgaggtggt gcagctcggc ggtggtgtcg agtgccgtct cgttgagctg
     1501 gcgtcgctgt tggcggatag gacctcggga aatgaggacc gcgtgcggct gaaactgctc
     1561 caaggcgcga cgcttttccc aggtggccgg gtcgaacggg cttggggtgt cctcaaggac
     1621 gcggccgaca tcggtcgggt cgagcagaac caggatgcgt cggcctggga tcaccagttc
     1681 caccgggccg gagccgaact gccgacgcaa tacctgcatc cgcctcaatg ccctgtggtc
     1741 gccctggact cgttcgagca gcccgaccac tgtccgccgt cgagcgatgg gtccggccgc
     1801 aagcgatgcc aaccccaaat cggctaacgc tgccaccgcg gctacaccgg tcagctgatg
     1861 ctgtgattgt tcagacacag cctgttgatg cccacatgag cgagggcaaa aacctgatgt
     1921 gtcggtgtcg gtgcttcgat tgcacgctct agaccgtggg cgatcgcacg cgcggcctgc
     1981 gcgtagcacc taggcttacg cgttggcgtc ggtctttgct ctggcgggaa gggcccctat
     2041 gtcacaacgt cgccgtatac ctcctgtcct gcacaacaac ggcttgctat tggcgtgcct
     2101 gggattgttc gcagtgttct tcgtcggcat ggtcgtatcc ggtacggcaa cctacaacca
     2161 ggagcagcag gagcacggct cacaacagca ggtgtcggtg gtgggctacc tgacgaccgg
     2221 agattttgtc gaggcgacgt tcgagaactg ggaatccgag ttcttgcaga tgggcatgta
     2281 cgtcgtgctg accgctttcc tgttccaaaa gggttcctcg gagtccaagc cgttgaacga
     2341 gtccgcaccg caggaccatg acccgcgaag gacggctgtg aatcagcaca cgccgtggcc
     2401 ggtacgcaag ggcggctggg tcctacgcgt gtatgagcac tcattggcga tcgcattctt
     2461 cgtattgttt ttcgcgtcgt gggcgctgca cgccgtcggc ggcgacaagg cgttcaacga
     2521 ggagcaggtg cagcacggcc agaccgcgat ctcggtgtgg cggtacgtaa cgacgtcgca
     2581 attctggttt gagtcgatgc agaactggca gagcgaattc atcgctgtcg cagccattgt
     2641 cggtctttcg atcttcttgc ggcagcgggg ctcagcagaa tccaagcccg ttgccgagcc
     2701 gcacggccac accagcgcct agcggccccg tgtactggtt tgccaccgcc acagtcagcg
     2761 tccgacgaat tgcgccattg cgtccaatag atcgggccgg aatgtcgccg gcgggatgag
     2821 gccgttttaa gcttgaacgc tttgtgccgc tgcacgattc gcgacttcgg ttgttccatg
     2881 gcggcagtgg gctattaact ctgctcatcg tcgttcctac tcgctccacc ttatgtaccc
     2941 attggctagc ggcaacatgg ccacaacaaa cctgtaaagc gccagagcga gacgcgggct
     3001 acggtcgcgg cgctccagca tggagtccgt cgaacgcgac cgggacatag cgctagtcgg
     3061 gcagcctgag cgggcgagcg atgatgtcgg cggccaccga ggagatggca agtttacgtg
     3121 cgtaggagta agcccgcagc ggttcggcgc gtggccagat caaacctgct cggcatgaat
     3181 gtcggcgttt ggccgagccg agagccgcgc ttcgcagtcg atcacgcgct ctccgggcat
     3241 tactaacgcg cttggtcaac gcacagatct cgacgcggtt cagccgaccg tctgtccgcc
     3301 gtcgacgacg agtgcgtggc cgacggtgaa ggcggtgttg ttggagcaga gccacatcac
     3361 tgccgaggcg atctcttcgg gacgacccat ccggccgacg ggttcctgat cgatgacccg
     3421 ctcgcgtcct tctggtgtgc ctcctgagaa gcgatccatc atgggggttt cgatgatgcc
     3481 ggggcagatg gcgttgatgc gcacgtttgc cgccgcgtag tccagggcgg cgcagcgggt
     3541 cagcccgatc accccgtgct tggcggcgcc gtaggctcca ccgccgggga agcccttgac
     3601 ccccgccccc gaggaggtgt tgatgatcgc gccgccgcct gcggccagga tcaacgggat
     3661 ctcgtatttc atgcacagaa acaccccacg cagatcgatg tcgaggacgc gttgccattg
     3721 gtcttcggtg atgtcggcca gcggagtggg gggctgctcg atgccggcgt tgttgaacgc
     3781 gaagtccagg cgaccgagcg cctcgacggt ctgccgaagg gcggtctgca catcactgct
     3841 gacagtgacg tcgcacgtga gtccgagcgc ctgcccaccg gtacgagcga tctcctcggc
     3901 gacaccacgg ctggcctcag ctgagacgtc ggcgatggcc actgcggcgc cacggtcggc
     3961 gaacgctagc gccgtggcgc gcccaatgcc gctcccggcg ccggtgatga acgcgacttt
     4021 gcccgcgaac tagtcgttcc gactattact ggtggtgctt atcggcatgg ctcgagttcc
     4081 tgacgtgggt cggtgaatcg tcagtgtgtg gtccgcggtt gtggtgaaga aactagttac
     4141 ccgtttcgca ggctgttctg aaagaagccg gtcagcttgt cgaacgggat gaggttgact
     4201 cggtcgtaga gatcgacgtg acccgcgccg gggatgatga ccagttcctt gggctcggcg
     4261 gcgagggcgt aggcgtcctc gctgaactcg agggagtggg cgttctggcc ggcgatgaac
     4321 agcatgggtc gtggcgagat cgtctcgatg tcgttgaagg ggtagaagtt catgaacttg
     4381 acgttgctgg tcatcgtcgg gtgcgtggtg gtgtc
//
LOCUS       DASYLX010000339         7488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_343489,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000339 DASYLX010000000
VERSION     DASYLX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343489"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(663..1598)
                     /gene="purU"
                     /locus_tag="VGP27_08255"
     CDS             complement(663..1598)
                     /gene="purU"
                     /locus_tag="VGP27_08255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEV7851341.1"
                     /translation="MEVLNGAHAADALDATQYPQVDSGTLPVKDLGRLLLRCVDRPGL
                     VAAVSTFLACAGANIISLDQHSTAQPGATFMQRTIFHLPGLATCRDALEDAFVEQVAE
                     PFNMEFKLTEAAKPKRVAIMASKDDHCLLDLLWRNRRGELDMSVVTVISNHPDLADKT
                     RPFGVPFLHVPASKDIRAQAEQCQLDVLRNNVDVVILARYMQILTPQFVSGVGCPLIN
                     IHHSFLPSFIGAAPYRRAKERGVKLVGATAHYVTETLDEGPIIEQDVVRVDHRHGVGD
                     LIRLGADVERAVLSRAVLWHCEDRIIQYGNQTIVF"
     gene            complement(1715..3349)
                     /locus_tag="VGP27_08260"
     CDS             complement(1715..3349)
                     /locus_tag="VGP27_08260"
                     /inference="COORDINATES: protein motif:HMM:NF044496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851342.1"
                     /translation="MGLVGSGLNQPLYGAIGIAGQVPIVNIFIANGTNGAPGTGANGG
                     NAGLLIGYGGTGGSGAPGQIGGNGGQGGLFFGNGGAGGVGGIGAAGGNGGNAGFLALF
                     SNGGDGGAGGYGGGAGGNGGSAGKFGLFGHGGNGGVGGNGTTGTASDPLMTLAAASLD
                     VTTLAAPAPAATGTTGANGTIVGTNGGNGTNPPGSGTAGTAGAAGVAGTGQSGLPGNP
                     GDPAGAGGAGGTAGPTGIGGNGGLGGSGTDSGNGGAGGAGGASSTAGVTAVGGNGGNA
                     GNGGTGTTNGGAGGAGGAAVNTGGAAYGGTGGTGGSGGIGAAGVAGGTGQAGAQGGPG
                     ANGTIVGTNGTNGGAAGAGGNGGTGGLFGRGGNAGNGGTGGTGGTGGAGQAGGAGGTG
                     GNGAQGGNGGNGDAGGNGGVGGAGGAGTGGAGVLGVGGRGGAGGSGATGGPGGAAGAG
                     GAGGLGGVGGTGAAGGTGGTGGTGGAAGTAGGGGIFGSRGSGGTTGSTGTTGANGATG
                     ATGATGNAGATGASGTAGANGAGGTAGGPGANGASS"
     gene            3870..4295
                     /locus_tag="VGP27_08265"
     CDS             3870..4295
                     /locus_tag="VGP27_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7851343.1"
                     /translation="MHESTSAPWWRYVVTVTGNAAQKDIAAATGIDQSSISRWQRGAN
                     TPRAEAVVGFARVYGRSPVEALVAAGYLSSTELGVVELTTLTGDLTGVSIDSLLSELR
                     RRVLAATRDDAQSWPPGWSADDPGMGRHQDGEQGGDLSG"
     gene            5662..5913
                     /locus_tag="VGP27_08270"
     CDS             5662..5913
                     /locus_tag="VGP27_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dinucleotide-binding-like protein"
                     /protein_id="HEV7851344.1"
                     /translation="MKAFNTLFRDVLAAGNPLDVFMAGDDAQAKASLSAFIESLGLRP
                     RDTGDLSVAHWLEGVGLLSVGLGRYGLGDFNFSLGVNVG"
     gene            complement(5900..6547)
                     /locus_tag="VGP27_08275"
     CDS             complement(5900..6547)
                     /locus_tag="VGP27_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7851345.1"
                     /translation="MTELEKGPRGLRRGRGARERILGASRQLFRDQGINSTGLNELCA
                     VAQVSKRTFYQHFTGKDELIAEHLRRFDPDILPEVFDRTDLTPRERLLAAFDIHAPLC
                     PFIAAAVEIHDPGHPARLSARDYKTAFAVRLTETAREAGATNPEQLGEQLALLLDGAS
                     ARNRVLDSDTFATAAAIAAVLIDNAIPAAAVPPGAGGNLSTGTGPRAGSRELSRR"
     gene            6674..7408
                     /locus_tag="VGP27_08280"
     CDS             6674..7408
                     /locus_tag="VGP27_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851346.1"
                     /translation="MGKLDGKVAVITGATSGMALAGAKLFVDEGAHVFISGRRKDALD
                     QAVELIGRNVTGVQGDSADLDDLDRLFDTVKQEKGAIDVLWASAGTGKQSRLGEITED
                     DFDAAFWLNARGTLFTVQKALPLFNDGGSIFMTGSNASLRGYANWSVYAGSKAVLPAY
                     ARVWVSELRDRKIRVNVLTPGQVASPMLAEVMDDEAKAQFESVIPRREMGRPEEIASA
                     ALFLASDDSSYVNGMELVVDGGTTVI"
BASE COUNT         1285 a         2453 c         2368 g         1382 t
ORIGIN      
        1 tcgggatcgg cggcgaactg ttgcgcggcc acctcgttgt tgaacagctc gagaatgtac
       61 tggatgacgg aatccatgcc acaggccttt cgcgttggtt aggagagctt tgccggatac
      121 ggccggtcac ttccagcaac gttatggacc ccgaccccca atgggcaggg ggtcggcgcc
      181 cgtgcttaca ggccgtgctc tagggggtcg cccggcttcc ccgctagggg attaggggat
      241 gcgtggggga tcggcactgc attcccagcc cgttcacagc gtgcctgcgc gtttcgggct
      301 ggaccggggc gtcgttttgt gtcgacggga gccggtacga gtcgggtgtc ctagcggatc
      361 tcttcgatgg cgttttttag gggctcggct gtgtgccgaa caccgcttag ccgttgttgc
      421 cgacctcgat gacgccctcg gccccaagac tcttctaggg gctaggtcca ccgtgatgat
      481 ttcaacgggt tctgacaaga aacgctggct ctccgcttcc ggacagggct ggcttcgtgc
      541 ttcccgattc gatgcgcatc tcgacctggc gtttcccgat attcacgtgc tgcgagctcg
      601 cccacgcgga tggtgcattc cggtgagtcg agacgaactt tgaggtcttt atagccatgc
      661 tttcagaaga cgatggtttg gttgccgtat tggatgatgc ggtcctcgca gtgccatagg
      721 acagccctgg ataagacggc gcgctcgacg tcggcgccga gccgtatcag atcgccaacg
      781 ccgtgacgat gatccactcg cacgacgtcc tgctcgatga tcggtccctc gtcgagggtt
      841 tcggtcacat agtgggcggt cgcgccaacg agtttgacac cgcgctcttt ggcccggcga
      901 tagggagccg caccgatgaa cgacggcagg aatgagtggt ggatgttgat cagtgggcat
      961 ccgacaccgg acacgaactg gggggtgagg atctgcatgt agcgggcaag gatgaccacg
     1021 tcgacgttgt ttcgcagcac gtccagctga cattgttctg cttgcgcccg aatgtctttg
     1081 gaggcgggca cgtgcaggaa cgggacgccg aacggacgtg tcttatcggc gaggtcggga
     1141 tggttggaga tcaccgtcac caccgacata tccagttcac cgcgacggtt gcgccacaac
     1201 aggtccagga ggcagtggtc gtccttcgac gccatgatcg cgactcgttt gggtttggcg
     1261 gcctccgtga gtttgaactc catgttgaat ggctcagcca cctgctcgac gaaagcgtcc
     1321 tccaacgcgt cccggcaggt cgctagtccc gggaggtgaa agatggtgcg ctgcatgaac
     1381 gttgcaccgg gctgcgcggt cgaatgctga tctagcgaaa tgatgttggc cccggcacag
     1441 gccaggaagg tgctgacggc ggccaccaat ccgggccgat cgacgcagcg caacaacaat
     1501 cggcctagat ccttgactgg caacgttccc gaatcgacct ggggatactg cgtggcgtct
     1561 aatgcgtctg ccgcgtgagc accgttgagg acttccatgg ctctaccgtt ccctcgatgc
     1621 ttgttggtgt ggcacgagat cgggggcgtc ggcatagccg acgcccccga tgacgttcag
     1681 cagcggcctt ttagaagccg tgccctattt cgtgttatga cgaggcgccg ttggcacctg
     1741 gtccgccggc tgtgccgcct gcgccgttgg cgcccgcagt accgctggct cctgtggccc
     1801 cggcgttgcc ggtagccccg gtggcaccgg tagcgccgtt ggcgccggtg gtgccggtac
     1861 tgccggtcgt gccgcccgat ccgcgactac cgaagattcc gccgccacca gctgtgcctg
     1921 ctgcaccgcc ggttccaccc gtcccgccgg tcccgcctgc agcgccggtt ccgcctacgc
     1981 cgccgaggcc gcctgcaccg cctgcacccg ctgcgccgcc cgggccgccg gtagcgcccg
     2041 aaccgcctgc gccgccgcgg ccgccgacac cgagaacgcc tgcgccgccc gtgcctgcgc
     2101 cgcctgcgcc gccgacaccg ccgttgccgc ctgcgtcgcc gttaccgccg ttgccacctt
     2161 gagctccgtt tccgccggtg ccacctgccc cgccggcctg acccgcaccg ccggttccgc
     2221 cggttccgcc ggtcccgccg ttgccggcgt tgccgccgcg accgaagagc ccgccggtgc
     2281 caccgttacc gcctgcgcct gccgcaccac cgttagtgcc gttggtaccg acgatggtgc
     2341 cgttggctcc tgggccgcct tgggccccgg cctggccggt cccccccgct acgccggcag
     2401 caccgattcc gccgctgccg ccagtgccgc cggtgccgcc ataagccgcc ccaccggtat
     2461 tcactgctgc gccgccggca ccgccagctc cgccgttggt agtaccggta ccgccgttac
     2521 cagcgttacc gccgttaccg ccgaccgccg ttacgcccgc agtgctcgag gcaccgccgg
     2581 caccaccggc gccgccgttg ccgctgtcgg ttccgcttcc gccgaggccg ccgttaccac
     2641 cgatgcctgt agggccagcg gttccgccgg caccgccagc gcctgcgggg tcacccgggt
     2701 tgcctgggag gccgctttga cctgtgcctg caacgccggc cgcacctgcg gtacccgcag
     2761 tgccgctccc cggcgggttc gtaccgttac cgccgttagt gcccactatg gtgccgttcg
     2821 cgcctgtcgt acccgttgct gccggggcgg gcgccgcaag ggttgtgacg tccaggctcg
     2881 cagccgccag ggtcatgagc gggtctgacg ccgtgcctgt cgtcccgtta ccaccaacgc
     2941 caccgttacc gccgtggccg aacagaccaa acttgcctgc acttccgccg ttaccgcccg
     3001 cgccgccgcc gtacccaccg gcaccgccgt cgccgccatt actgaacagc gccaggaacc
     3061 ccgcgtttcc gccgttgcca cccgctgcgc cgatcccgcc gacaccgccg gctccgccgt
     3121 tgccgaagaa caacccgcct tggccgccgt tacctccgat ctgtcccggg gcgccggacc
     3181 cgccagtacc gccgtagccg atcagcagac cggcgttgcc gccattagcg cccgtgccgg
     3241 gcgcaccatt agtcccgttg gcgatgaaga tattgacgat cgggacttgc cccgcgattc
     3301 caatagcgcc gtacaacggc tgattcaggc cggaaccaac tagtcccact gataacgcgt
     3361 tcggcgtggg cgtgttcggc gacagcgcaa acaggctatg aggggagcat ccagcagaag
     3421 gcagtacgca cgacgtcgct ggtgacagcg agggcacgac cgctggcgcc gcctgcgctg
     3481 tgccagcgcc aagcgctaaa gcgacgccgg cgccggcgat cacaagtccc gttgcgcacg
     3541 ggtagacgta atttttcatt ttgaaagttc ctattcaggc tttctaaagc cgaaccgcag
     3601 attgtgacgt gagacgtcat tggcgccgat ggctgtagcg gcgaaaccgc taggagcccc
     3661 tttctatctc agtccagcgc gtatgcatgt gaccataaca tcatgcgtgc atgcatgcaa
     3721 gacgaagaga gtttgctgaa gcctttcgga gcgcatcatg cgccggcgcg agcgccgtcc
     3781 ggaacttcga ccggaatctg agttgacgat caaattgctg gggaagccca gaacctactc
     3841 acagcggatg ccatactctg tcgtacgtga tgcatgagtc gacatcagcg ccatggtggc
     3901 gctatgtcgt gacggtgaca ggtaatgcag cccagaagga tattgcggcc gcaacaggca
     3961 ttgaccagtc cagcatctcc cgctggcaac gtggcgccaa taccccccgg gccgaagccg
     4021 tcgtcggctt tgcccgggtc tacggtcggt cgccggtcga ggcgctggtg gccgcgggct
     4081 atctgtccag cacggagttg ggcgttgtgg aactcactac cctcaccggg gacctgaccg
     4141 gggtgtcaat cgattcgcta ttaagcgagt tgaggcgtcg ggttcttgcc gcgacccgcg
     4201 atgacgcgca atcgtggccg cccggttggt ccgccgatga cccgggaatg ggccggcatc
     4261 aagacggcga gcagggcggc gacctcagcg gctgacagat cctccggccg cacccgcgac
     4321 atcacctgct ccagctccca ccgcagatga ggcaccaccg catctgcgcc cgtcttcaca
     4381 aagctgcaag ccgcgttgat gcgttcgcct acctccgaca agtccgtctc gaacaaaaga
     4441 cacatcccgc ctttcaagat cacatgcaaa acgtgcgaaa atagaatttg atgattcacc
     4501 ccagttattt gagggtgacg tcagaagagt agtgcggcgg agctcgcatc ccacgcggca
     4561 acaacccaat ccttgcaccc caccgcagta tttcgcaagc aatcgtaatt aatgccaagt
     4621 aatagcgctg tggcaactcc aggagagctg aatactccta gctcagagca gtctgaggcg
     4681 tccagagcaa aaaaacctgc gaaactactg gctaacattc acccagccgc gccacgccat
     4741 ccacgcgcac caacgacatg ctctactcaa gtccgcttca ctgcggcctg gcgccgcagc
     4801 gtcgctctgg cccgaaccgt gttcgaaaga actcgcgggg ccacttgcaa tcgtatttcg
     4861 atccagcagc tcttcccgct cacacggccg acataacgaa gatcagaacg tatgggcaat
     4921 cgacagagat cgatcagctg gggacacccg atcactgaac gtgcgaaaga cctcacaatt
     4981 acctgtcggc atccgacagt tctgtacagc tcgggcagct ccatggctga cacaatccac
     5041 tgggcccctc gaggcttgcc tgcgaacacg ttgcggcact tggtaaatcc gttatggcga
     5101 caggccagac agtaagggca gcgtcttcgg ccggtaatga cgctggacgc aatccgcacc
     5161 gaacgcctcg cgttcgcaga accgtgagca gtgcgtcaca tcatgggttc gcttcggtgt
     5221 tcgggcggag cgcaacggtg gtcacccccg gcgtcgacac cgtcgagtgg caatcttggc
     5281 gtcctcggcg gagcaccttc tcaagggtca actgccgctg cggcgacacc ttccggcctc
     5341 acccggccgc cgactatata gctgcgcacg gatccggtgt ttcaacatgt ccgctgcggc
     5401 cccttcgaca ctgcaaccga tagcactatc tcgcaagtag gtagcgcgtt ttttaccaaa
     5461 cgcgtcaata tcggcaaatg actgctcaac tgcttatgag ggatcgcgcg aatccgacgc
     5521 ccacccacat cccgattttg cgcaataggc cttggtcgac atcaccaacc cgttcaaccc
     5581 cagcgtcacc gggctggccg tccctgacga cacttgcatc gcgcagatgg tcgccgaggc
     5641 cgccccgcag gcgcccatgt cgtgaaggcg ttcaacaccc tcttccgcga cgtcctggcc
     5701 gcgggcaatc cgctggacgt gttcatggcc ggcgatgacg cgcaggccaa ggcgagcctg
     5761 tcagcgttca tcgagagcct cgggctgcgc ccgcgggaca ccggcgacct gagcgtggcg
     5821 cactggttgg agggcgtggg ccttctgtca gttggcctgg gccgctatgg cctaggggac
     5881 ttcaacttct cccttggcgt caacgtcggc tgagctcgcg ggatccggcg cgcggccccg
     5941 tcccggttga caggttcccg cctgcgccgg gcggtaccgc tgccgcggga atggcgttgt
     6001 cgatgaggac ggctgcgatc gcggcggcgg tggcgaaggt gtcggagtcg aggactcggt
     6061 tgcgggccga ggcgccatcc aggagcagcg ccagttgttc gccgagctgt tcggggttgg
     6121 tggcgccggc ctcgcgggca gtttcggtga gccgcacggc aaaggccgtc ttgtagtcgc
     6181 gagcgcttag gcgtgcgggg tggcccgggt cgtgaatttc gacggccgcc gcgatgaacg
     6241 ggcacaaagg cgcgtggatg tcgaaggcgg caaggagccg ttcgcggggt gtcaggtcgg
     6301 tgcggtcgaa cacttcgggc aggatgtctg gatcgaatcg gcgtaggtgt tcggcgatca
     6361 gctcatcctt gccggtgaag tgctggtaaa acgtgcgctt ggacacctgg gccaccgcgc
     6421 agagctcgtt caggccggtg ctgttgatgc cttgatcgcg gaacagctgt cgtgaagcgc
     6481 cgaggatgcg ctctcgtgcg cccctgccgc ggcgcaggcc ccgcggcccc ttctctaact
     6541 ccgtcataca accagcgtag cccggttggg taccgaccgg tgtgcatagc ttgcactcgc
     6601 catcgccgtc cgatacgtta agcacgatga tcggtgtaca taacatcggc actatcgaat
     6661 gaatggagtg atcgtgggaa agctcgatgg caaggtcgcg gtgatcaccg gcgcgacaag
     6721 tggtatggcg ctggccggtg ccaagttgtt cgttgacgaa ggagctcacg tcttcatctc
     6781 gggccgacgg aaggacgcgc tggatcaggc cgtcgaactg atcgggcgga acgtgaccgg
     6841 tgtgcagggc gattcggccg acctggacga tctggaccgt ttgttcgaca ccgtcaaaca
     6901 ggaaaagggc gcgatcgacg tgttgtgggc gagcgccggc acgggcaagc agagtcggct
     6961 cggcgagatc accgaggatg acttcgatgc cgccttctgg ctgaatgccc gcggcacgct
     7021 attcacggtc cagaaggcgc tgccgctgtt caacgatggc ggctcgatct tcatgaccgg
     7081 gtcaaacgct tcgctccggg gctacgccaa ttggagtgtg tacgcgggaa gcaaggccgt
     7141 gctgcccgcc tacgcgcggg tgtgggtgtc cgagttgagg gacaggaaga tccgggtgaa
     7201 cgtgctgacc cccggccagg tcgcctcgcc catgttggcg gaggtgatgg acgacgaggc
     7261 gaaggcacag ttcgagtccg tgatcccgcg gcgagagatg ggccgccccg aggagatcgc
     7321 gtcggccgcg ttgttcctcg cctccgacga ctccagctat gtcaatggca tggagctggt
     7381 ggtcgacggc ggcaccacag tcatctgagt gagctacgac aacaccgaag gagtaatcat
     7441 gaccacgttc gccccgcacc gcgacacggt gcaggccttc gtcgacta
//
LOCUS       DASYLX010000340         7828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2867467,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000340 DASYLX010000000
VERSION     DASYLX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867467"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGP27_08285"
     CDS             complement(<1..193)
                     /locus_tag="VGP27_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase, NADPH:quinone
                     reductase"
                     /protein_id="HEV7851347.1"
                     /translation="MRAIQIEQFGDPAQVIRVVDIEEPPPPGPHEVLVSVEVSPLNKH
                     DLLVVSGVLARPPLPHIPGA"
     gene            complement(284..742)
                     /locus_tag="VGP27_08290"
     CDS             complement(284..742)
                     /locus_tag="VGP27_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851348.1"
                     /translation="MSTIHFHRSTTASAEQFIAGLTDFGPGRSGLFPNSADGDLEVHY
                     QGACDADVTEGSGGIWERLYYDWSDPHRVTLTTTDSNTWAGASGYVYTLSPRPDGTTE
                     VDVVIVREGKTFKGRVLAAVLATIGKGVLRKAFVHSVKAIETRSDAGIRR"
     gene            879..1682
                     /locus_tag="VGP27_08295"
     CDS             879..1682
                     /locus_tag="VGP27_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7851349.1"
                     /translation="MSVTASALGDFLRARREQLHPEDVGLSSGPRRRVPGLRREELAM
                     LAGISVDYYLRLEQGRDAHPSAQVLDALARALRLDVQATEHLHRLAGLRAAHSPRTVA
                     ETAADGLDALIDQIPLPAIVTNRYQDVLAANAVAQKLSPGFTPGENFLRWRLLAAAAR
                     DFFVDWDEATEVAVSGLRESAAGDLGDPRLRALIDELSAASDRFRELWARADVGYRTG
                     ILHVRHPVAGDLHLRRTRLTVPDSDTQVFIYHPEPGSESAKALKALAAS"
     gene            complement(1741..2874)
                     /locus_tag="VGP27_08300"
     CDS             complement(1741..2874)
                     /locus_tag="VGP27_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV7851350.1"
                     /translation="MRLVRPQLATQIRIIAAKGRADGMQTDHCEPDRRSVKKMQTMRS
                     VVADTLGEPSEVLHLQSRPIPEPGPGQVRIRVAAAPVEASDLHTIRGRYGFTPEFPTV
                     PGIESVGVIDGLGTGTDGLTVGQRVMTIGVTGTWQDYVVADTGRVLPVPAGMSDSTAA
                     QLLSNPLTAVILTGDELDVRPGEWLLQTAAGSIVGQSVIQLGAHVGFKTLNVVRRRSA
                     VEDILALGGTAVICTEDEDLRERVADIAGHDGVSKAIDCVSGQVGADVSRALAPGGEL
                     VVYGALSTHRQTEPDKLTIPIFARSLIYETKTVRGFWLFRWFSATPKDRMAAAIDRTV
                     QLADSGALCVPQGQPIPVEKFSEAVYLAEAADHGGKPLLVFGP"
     gene            3063..3242
                     /locus_tag="VGP27_08305"
     CDS             3063..3242
                     /locus_tag="VGP27_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7851351.1"
                     /translation="MLGNKEVAARLFVSPRTVQAHRTHVYTKLGLTSRARHAQETARH
                     NCHNYVRLRELTGSS"
     gene            3615..4775
                     /locus_tag="VGP27_08310"
     CDS             3615..4775
                     /locus_tag="VGP27_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851352.1"
                     /translation="MSNAVMYERTLFEPEHELFRESYRTFLQRHVAPYHAQWEEDKLV
                     DRGVWLEAGKLGFLGTAVPEEFGGGGNPDFRFNVIVTEETTAGRYSGIGFALHNDIVA
                     PYLMRLCTEEQKHRWLPKFCSGEMITAIAMTEPGAGSDLLGINTRAVPDGDGWVLNGS
                     KTFITNGIHADLVIVVAQTSREKNPLGFSLLVVERGMEGFERGRKLDKIGMVAQDTAE
                     LSFEDVRVPRENLLGKVGKGFFYLMQNLPQERLSIALMSAAAMESSLKLTLDYTSQRK
                     AFGQSIGSFQYNRILLAELATEATVVRVLIDEFIRLHLEEKLTAEQAAMAKWYATESY
                     VRLVDRCVQLHGGYGYMREYPIARAYLDARVQTIYGGTTEIMKEIIGNRLGV"
     gene            complement(4795..6582)
                     /locus_tag="VGP27_08315"
     CDS             complement(4795..6582)
                     /locus_tag="VGP27_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7851353.1"
                     /translation="MKGASAIRPKYETLPEALAAAAGTATNLTFVNANEEDQEVPMAR
                     IREKALSIAADLSARGVRQGDCVALVLPTCPAFVECFFGVMLAGAIPVPLYPPVRLGK
                     FDEYHRKTAAMLKAVDAVLVVTDQRIRRFLGVAVEKAAPRLGCVTASDLGIADAVEVG
                     PGPDDIAFIQFSSGTTHDPKPVALTHENLLYNLATIGNYLEGVGTPIPVGISWLPLYH
                     DMGLIGNLLSAFYIPAPLVLLPPELFVTRPGAWLRAISRHRGTSSAAPNFAFGLCVNR
                     ITDDELDGVDLSSWRLCLNGAEAVSAAMQRQFSERFERWGFDASALTPCYGMAEASLA
                     VAFKPAGTRFKTLAVDNDTLASKGVVEPGSKELVSVGVPLAGVEVEIRDDQSQPAPPD
                     HSGRIFVNSPSVMAGYYRRKDLTDETLQDGWLDTGDLGFVHDGELFVCGRRKDTVIIR
                     GANHAPQDFEVALEGLPGVRTGCAVAVGFVPEGKEEESLAMLVETTRDAPPTLASDVS
                     ARVVERTSILPAHVELLVPGTLPRTSSGKMRRREARTQWLEGKLAPPKTVSPVRLMWY
                     AAKGQLSHARAALVRSNAASPAVDQLERS"
     gene            complement(6579..6842)
                     /locus_tag="VGP27_08320"
     CDS             complement(6579..6842)
                     /locus_tag="VGP27_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV7851354.1"
                     /translation="MTSTEQNEKLNAVIQDLIDMIKAESPGVNLTVDSGLQDGGLDSL
                     KVMSLVLRIESRYGIELDADDDDELRTLADLARLVQRRIEKQP"
     gene            complement(6937..>7828)
                     /locus_tag="VGP27_08325"
     CDS             complement(6937..>7828)
                     /locus_tag="VGP27_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV7851355.1"
                     /translation="EYRHHTAIDESNVDMMERAASGLADRHGAEVLADVDVLLTHSQM
                     PDLPIVGAGGEVASRLGMNPQVIIDVHNSGCASFIYMVNLARRLLQSGAGSTAMIACA
                     ATAAGKIFEQEQVRKLAQASVPGDGAGVGLLTLSDTSPVLDVEARFYGQNAVDMTLET
                     EPPRRWWEAGPGEGHVGFNEGKIMKVLSRGNRMVPEVVHAVCDRIGVKPGDLDLLVTN
                     QPNRLLLRNWAEALELPRERHRDTFQQCGNLFGVGIPVSLESAIDDGQLKAGDVVMMA
                     GFANAGDFAGAAAIQWGGRP"
BASE COUNT         1443 a         2441 c         2495 g         1449 t
ORIGIN      
        1 ctgcgccggg tatgtggggc aggggtggtc gcgccagcac gccgctgaca accagtaggt
       61 cgtgtttgtt caacggcgag acctcaacgc tcaccagaac ctcatgcggg cccggtggcg
      121 gcggctcctc gatgtcgacg acgcggatca cctgtgcggg gtcgccgaac tgttcaatct
      181 ggatggcacg catatcaatt cctctccatc gaaggttagg cagctgtgtg atcatgccta
      241 acggatacgt gattttggct aggctggtta attttgacag cagttatcgc cgaataccgg
      301 cgtcgctgcg tgtttcgatg gccttgacgc tgtggacgaa tgccttccgt agtacgccct
      361 tgccgatcgt tgcgagtacg gccgccagga cgcgtccctt gaaggtcttg ccctcgcgga
      421 cgatgacaac gtccacctcc gtggtgccgt cgggacgcgg gctgagggtg tagacgtagc
      481 ccgatgcccc ggcccaggtg tttgagtcgg tggtcgtcag cgtgacgcgg tggggatcgg
      541 accagtcgta gtacaggcgt tcccagatgc cgccggagcc ttcggtgaca tcggcgtcac
      601 acgcgccctg gtagtgcact tcgaggtcgc catcggcgct attgggaaac agcccggagc
      661 ggcccggtcc gaagtcggtg agcccggcga tgaactgctc agcgctggcg gttgtcgagc
      721 ggtggaagtg aatggtggac atggtgtcct ccttgtgtga cggagtttgt cgttcgtcca
      781 cgggaagcgc atcgccgcca gggcgaattc ctgtgtggag ctcggcgggg aggccttgtt
      841 gatcctggta ttgccgggga cagccttagg atcgggccat gtcggtgacg gccagcgcgc
      901 tcggtgactt cctgcgcgca cgccgcgaac aactccaccc cgaggacgtc gggttgagtt
      961 ccggaccgcg caggcgcgtg ccgggattgc gccgcgagga attggcgatg ctcgccggga
     1021 tcagcgtcga ctactacctg cgactcgagc aaggccgtga cgcgcacccg tcggcacagg
     1081 tgctcgatgc gctcgcccgg gcgctgcgac tcgatgtcca agccaccgaa cacctgcatc
     1141 ggcttgcggg gttgcgggcg gcgcatagcc ctcgaactgt tgcagagacg gcagctgacg
     1201 gtctggatgc gctgatcgac cagatcccgc ttccggcgat cgtcaccaac cgataccagg
     1261 atgtattggc cgccaacgcg gttgcccaaa agctgtcacc agggttcacc cccggcgaga
     1321 acttcttgag gtggcggctg ctggcagcgg ctgcccgcga tttcttcgtc gattgggacg
     1381 aggccaccga ggtcgcggtc agtgggttac gggaatccgc ggccggcgat ctgggggatc
     1441 cccgcctacg cgccctgatc gacgaactgt cggcagccag cgaccggttc cgcgaactct
     1501 gggcacgcgc agatgtcggc taccgcaccg gcatcctcca cgtgcgacac cccgtagcgg
     1561 gtgatctcca tctacgccgc accaggctca ccgttcccga ctccgacacc caggtgttca
     1621 tataccaccc cgagccgggc agtgaatcgg ccaaagcact caaggcactc gcagcgtcat
     1681 aacccgaaaa gcgcagaaat ctcgattcgc atcgcgggca ggccatgggg cgtattttca
     1741 ctacggtccg aacaccagca gcggtttgcc tccgtggtcc gcagcctcgg ccaaatagac
     1801 ggcttcgctg aacttttcga cgggaatcgg ctggccctgc ggcacgcaca acgcgccgct
     1861 gtcggcgagc tgaactgtcc ggtcgatggc tgcggccatc cgatccttcg gagtcgcgct
     1921 gaaccagcgg aacagccaga acccccgaac ggtcttggtt tcgtagatca gtgagcgcgc
     1981 gaaaatgggg atggtgagct tgtcgggttc ggtttggcgg tgcgtggaca atgccccgta
     2041 gacgaccagc tcgccgcccg gtgccagcgc acgcgacacg tcggcaccca cttgaccgct
     2101 gacacagtcg atcgccttgg atacgccgtc gtggccagcg atgtcggcca cccgttcgcg
     2161 caggtcttca tcctccgtgc agatcaccgc cgtgcccccc agcgcgagga tgtcctcgac
     2221 agcggatcga cggcggacca cattgagggt cttgaagccg acgtgcgccc cgagctggat
     2281 gaccgactgg cccacgattg aaccggccgc ggtctgcagg agccattcac cgggtcggac
     2341 atcgagttca tctccggtca agatgacggc ggtgagcgga ttgctgagaa gctgggcggc
     2401 cgtggagtcg ctcatgccgg ctggaacggg caggacccgc ccggtatcgg cgacgacata
     2461 gtcctgccac gtgcccgtca cgccgatcgt catgacgcgt tgaccgacgg tcagaccgtc
     2521 tgtgccggtg ccgagtccgt cgatcacacc gacggattcg attcccggta cggtgggaaa
     2581 ctcgggggtg aatccgtatc ggccgcggat ggtatgcaga tcgctggcct cgactggtgc
     2641 agctgccact cggatccgga cctggccggg accgggctcc ggaatcggac ggctctgcag
     2701 gtgcagaacc tccgacggtt cgcccagcgt gtcagcgacg acagaccgca tcgtttgcat
     2761 cttcttgacg ctccttcggt cgggctcaca gtgatcggtc tgcatcccgt cggcccggcc
     2821 cttggcggca atgattcgga tctgcgtggc caattgcggc cgcactagtc gcatgctgcc
     2881 actgccgcgg gtttttgcgg tccagtctcg cccggcgtcg gacacatgat gcatgccctc
     2941 caggtgcaaa gaggaactgc actcacattt gagaagcaga gaacgttcga ttcgacacga
     3001 ttgtcgagca gaattgcctt cgtagggggt tatggcagga cggtccaaga cggcaggttg
     3061 tcatccttgg caacaaggaa gtcgccgcgc ggttgttcgt ctcacctcgc acggtgcaag
     3121 cccatcgcac ccacgtgtac accaagctcg gcttgacctc gcgggcgcga cacgcgcaag
     3181 agaccgcgcg ccacaactgt cacaactacg tgcggttgcg ggagttgacg ggcagctcgt
     3241 gacgattacc tcaggttcaa ggggtcgaga gtcactgcaa gcagacgaat tcaacctcag
     3301 tccgaccagt accgcgttca cgggttcggc cccattctgt cgcggttaga tcagcgaaca
     3361 ccatatgatt cgccaggtta ggcttggttt ggtccaggtg atgttgccga tcggctcgac
     3421 cacacaggtc gctggtacca cgtagcggtt tcagccgggt catcgaaaca attgttgaag
     3481 cagtatgccg cagtaaaccc gaattcaaga aaatccgtgt caaaaacgcc ggaaagtgtg
     3541 aaacaaccgg ttgtcacggt caacagcgtt aaaatttgct ggtgtaaggg aagctacggg
     3601 ggagggtcgc cgccatgagc aacgccgtga tgtatgagcg gacgctattc gagccggagc
     3661 acgagctgtt tcgcgagtcc taccgaacgt tcctgcagcg ccatgtcgcc ccgtaccacg
     3721 cgcagtggga agaggacaag ctcgtcgatc gcggggtctg gctggaggcc ggcaagctgg
     3781 gctttctggg caccgcggtt cctgaggaat tcggtggcgg gggcaacccc gacttccggt
     3841 tcaacgtcat cgtcaccgag gaaaccacag cgggcaggta cagcgggatc ggcttcgcgc
     3901 tgcacaacga tatcgtcgcg ccctatctga tgcggttgtg caccgaggag caaaagcacc
     3961 gttggctgcc gaaattctgc tcgggcgaaa tgatcaccgc aatcgcgatg accgagccgg
     4021 gcgcaggaag cgatctgctc ggcatcaaca ccagggcggt tcccgacggt gacggctggg
     4081 tgctcaacgg ttcaaagacg ttcatcacca atgggattca cgccgatctg gttatcgtgg
     4141 tcgcgcagac aagccgcgaa aagaacccgc tgggattctc cttgctcgtt gtggagcggg
     4201 gcatggaggg attcgagcgc ggtcgcaagc tggacaaaat cgggatggtg gcacaggaca
     4261 ccgcggagtt gtcattcgag gatgtgcggg tgccgcggga gaacttgctc ggaaaggtcg
     4321 gcaagggttt cttctacctg atgcagaacc ttccgcaaga gcggctctcg atcgccctga
     4381 tgtcggcggc cgcgatggag tcgtcactga agttgacgct ggattacacc agccaacgca
     4441 aggcttttgg ccaatccatc ggcagcttcc agtacaaccg catcctgctc gccgaattgg
     4501 cgacggaggc gaccgtggtg cgagtcctga tcgacgagtt catccgattg cacctcgagg
     4561 agaagctcac cgctgaacag gctgcaatgg ccaagtggta cgcgaccgag tcatacgtac
     4621 ggctggtcga ccgctgtgtg caactccacg gcggctatgg atacatgcgc gaatacccga
     4681 ttgcgcgagc gtacctcgac gctcgcgtac agacgatcta cggcggcaca acggagatca
     4741 tgaaggaaat catcggaaac cgcctcggcg tatagcggcg aggcggcccg tcgatcaact
     4801 ccgttcgagt tgatcgacgg ctgggctggc ggcgttggac cgcacgagcg cggcgcgcgc
     4861 gtgggagagt tggcccttgg ccgcgtacca catcagccgc accggagaga ccgtcttcgg
     4921 gggagcgagc ttgccttcca gccattgtgt gcgcgcctcg cgccggcgca tttttccact
     4981 ggaggtccgg ggtaatgtac ccggcaccag cagctcgaca tgggcgggca gaatgctggt
     5041 gcgctcgacg acacgtgccg acacatcgct cgccaaggtc ggcggggcgt cgcgcgtggt
     5101 ttctacgagc attgccaacg actcttcctc tttcccttca gggacgaagc ccacggcgac
     5161 ggcgcatccc gtccgcacgc caggcaaccc ctccagcgcc acctcgaaat cctgtggggc
     5221 gtggttggcg ccgcggatga tcaccgtgtc cttgcgccgg ccgcacacga aaagctcacc
     5281 gtcgtggacg aagccgaggt ctccggtgtc gagccatccg tcctgcagtg tttcatccgt
     5341 cagatctttg cgacggtagt aaccggccat gacgctgggg ctattgacga agatgcggcc
     5401 gctgtggtca gggggcgcgg gctgtgactg gtcgtcgcgg atctcgacct cgactcccgc
     5461 caacggaacg ccgacgctca ccagctcttt gctgcccggc tcgacgacac ccttcgaagc
     5521 cagagtgtca ttgtctaccg caagggtttt gaacctcgtg ccggcaggtt tgaaggccac
     5581 ggccagtgag gcctccgcca ttccgtaaca cggtgtcaga gccgatgcgt cgaagcccca
     5641 acgctcgaac cgctcgctga attgccgctg catcgcggcg ctgaccgcct ccgcgccatt
     5701 caggcacaac cgccacgatg acaggtcgac gccgtcgagt tcgtcgtcgg tgatccggtt
     5761 gacacacaat ccgaatgcga agttgggcgc cgccgagctg gtgccgcggt gtcgtgagat
     5821 cgcccgcagc cacgctcccg gcctcgtgac gaacagttcg ggcggaagca gcaccagtgg
     5881 agccggtata tagaacgccg acagcaggtt gccgatcaag cccatgtcgt gatagagcgg
     5941 cagccaggag atgcccaccg ggatcggtgt cccgacgcct tcgaggtaat tcccgatcgt
     6001 ggcgaggttg tacagcaggt tctcgtgggt gagcgcgacc ggtttcggat catgcgttgt
     6061 gccagaggag aattggatga aggcgatgtc gtcgggacca gggccgacct ccaccgcgtc
     6121 cgcgatgccc aggtcggacg cggtcacaca gcccaaccga ggcgccgcct tctccactgc
     6181 caccccgagg aaacggcgaa tacgctggtc ggtcaccacc agaacggcgt ccaccgcctt
     6241 gagcatcgcc gccgtcttgc ggtgatactc gtcaaacttg ccaagccgca ccggcgggta
     6301 cagcggcacc gggatagcgc ccgccaacat cacaccgaag aagcactcga cgaaggcggg
     6361 acaggtcggc aataccagcg ccacgcaatc accctgccgc acaccgcgtg cggagagatc
     6421 ggcggcgata gacaacgcct tctcccgtat ccgggccatc gggacttctt gatcttcctc
     6481 gttggcgttg acgaaggtca gattcgtcgc ggttcccgct gcggcggcca gggcctcggg
     6541 gagcgtctcg tacttcggcc ggatcgcgga cgcgcctttc atggttgctt ctcgatacga
     6601 cgctgtacaa ggcgcgcaag atcggccaaa gtgcgtaact cgtcatcgtc gtcggcatcc
     6661 aattcgatgc cgtagcggga ctcgatcctc aagacgaggg acatgacttt cagcgaatcc
     6721 agtcctccgt cctgcaaacc cgaatcaact gtcagattca cgcctggcga ttcggccttg
     6781 atcatgtcga tcagatcctg aatcaccgca ttcaacttct cgttctgctc agtagatgtc
     6841 atggaaatgc ttccccctag cgttggtgcc ggtgcgcgca cgcgcactga ccgatgcgga
     6901 attgctgtgt gcagtcaaag tattggcgcg cggaactcat ggcctcccgc cccattggat
     6961 ggcggcggca ccggcgaagt cgccggcgtt cgcgaatcca gccatcatca cgacgtcgcc
     7021 cgctttgagt tgtccgtcgt cgatggccga ctccaggctg acgggtatac cgacgccgaa
     7081 gaggttgccg cactgttgaa atgtgtcgcg gtgacgttcg cgaggaagct ccagcgcctc
     7141 ggcccagttg cgcaacagca gccggttcgg ctgattggtg accaggaggt ccaggtcccc
     7201 gggcttaact ccgatccggt cgcacactgc gtgcacgact tcgggcacca tccgattccc
     7261 gcgggaaagc accttcatga tcttgccctc gttgaagccg acgtggccct cgccggggcc
     7321 cgcctcccac caccggcgcg gcggctcggt ttccagggtc atgtcgacag cgttctgccc
     7381 gtagaaacga gcttcgacat cgagcaccgg agatgtgtcg gacaaggtga gcaggccaac
     7441 acctgccccg tcaccgggga ctgacgcttg cgcgagtttg cgaacctgct cctgctcgaa
     7501 gatcttgcct gcggcggtgg ccgcgcaggc gatcatcgcc gtacttccgg cgccggactg
     7561 cagcagcctg cgggccaggt tgaccatgta gatgaacgag gcacagccgc tgttgtgcac
     7621 gtcgatgatg acctggggat tcatgccgag tcggctggcc acttcgccgc ccgctcccac
     7681 gatgggcagg tcgggcatct gagagtgggt cagcagcacg tcgacgtcgg caagcacctc
     7741 ggcgccgtga cgatcagcca gtcccgaagc ggcccgctcc atcatgtcga cgttggactc
     7801 atcgatcgcc gtgtgatgcc gatactcc
//
LOCUS       DASYLX010000341         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1440778,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000341 DASYLX010000000
VERSION     DASYLX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440778"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VGP27_08330"
     CDS             complement(<1..1983)
                     /locus_tag="VGP27_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV7851356.1"
                     /translation="MTGTDPYLWLEDITGDDALDWVRMHNEPTLAELGSERFEQMCTE
                     ALEVLDTDARIPYVRRRGDWLYNFWRDASNPRGLWRRTRLDSYRTDTPDWEVVIDLDA
                     LAAEEDDNWVWAGAKVIEPDHSLALVTLSRGGSDAAVVREFDMRTREFVADGFQVPEA
                     KTQISWEDENSVLIGTDFGEDSLTESGYPRLVKRWRRGTPLDDAETLFSGARTDVVVA
                     ASRDRTPGFERTLLSRAVDFFNDEVHELRDGEFIRIDAPTDATVSAHRDWLLIELRTD
                     WHTGAGSYRAGSLIAADYDEFLSGTAELYVVFEPDEHTSLHHYAWTRDKLVIVTLADV
                     ASRVQTVTPGTWTTEPVPGIPDNTNAVIVDTDDLGDEIFLDCSGFTTPSRLLHGPVDG
                     PLEQIKSAPAFFDADDITVTQHFATSKDGTAIPYFVVAPTDSAGPGPTLLGGYGGFEV
                     SRTPGYDGVLGRLWLARGGTYVLANIRGGGEYGPGWHTQAMREGRHLVAEDFASVAQD
                     LVRRGITTVSKLGAQGGSNGGLLMGIMLTQYPESFGALVCSVPLLDMRRFHLLLAGAS
                     WVAEYGDPDDPDDWQFIAEYSPYQNISAQRRYPPLLMTTSTRDDRVHPGHARKMTAAL
                     EAAGHHVHYYENIEGGHAGAADNAQTAFRSALIFEFL"
     gene            complement(2008..>2296)
                     /locus_tag="VGP27_08335"
     CDS             complement(2008..>2296)
                     /locus_tag="VGP27_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7851357.1"
                     /translation="FGSMNGWAADLIGQDVADKVGKVWGLGSDTAKDPGPWEGEQRNM
                     WKPTQQENLWFHGGNLHQSRHYSLYLALQLKARHEGIPTPVYGLQEVHHLS"
BASE COUNT          342 a          757 c          784 g          413 t
ORIGIN      
        1 caggaactcg aagatcagcg cggaccgaaa tgccgtctgc gcgttgtccg ccgcgccggc
       61 gtggccgccc tcgatgttct cgtagtagtg cacgtggtgc ccggccgcct ccagcgccgc
      121 ggtcatcttg cgcgcgtgcc cgggatggac acgatcgtcg cgggtcgacg tggtcatcag
      181 cagtggcgga tagcggcgct gtgccgagat gttctgatag ggcgagtact cggcgatgaa
      241 ctgccagtcg tcgggatcgt ctgggtcgcc gtattccgcc acccaggatg cgcccgccag
      301 cagcaggtga aaccgccgca tatccagcag cgggacactg cacaccagcg ccccgaacga
      361 ctccgggtac tgggtcagca tgatgcccat cagcagtccg ccgttgctgc cgccctgcgc
      421 gccgagcttg ctgaccgtgg tgatcccgcg ccgcaccaga tcctgtgcga ccgaggcgaa
      481 atcctccgcg accaggtggc ggccctcccg cattgcctgg gtgtgccagc caggcccgta
      541 ctcgccgccg ccgcggatgt tggccagcac gtaggtgccg ccgcgggcca gccacagccg
      601 ccccagcacc ccgtcgtagc cgggcgtccg cgatacctcg aaaccgccgt agccgccgag
      661 cagtgtcggt ccgggcccgg cgctgtcggt cggcgcgaca acgaagtagg gaatcgcggt
      721 gccgtccttc gacgtcgcga aatgctgtgt gacggtgatg tcgtcggcat cgaagaacgc
      781 cggtgcggac ttgatctgct cgagcgggcc atcgacgggt ccgtgcagaa gccgcgaggg
      841 ggtggtgaat ccgctgcaat ccaggaagat ctcgtcgccg aggtcgtcgg tgtcgacgat
      901 gacggcgttg gtgttgtccg ggatgcccgg tacgggctcg gtggtccacg tccctggcgt
      961 caccgtctgc accctgctgg cgacatcggc gagcgtgacg atcaccagct tgtccctcgt
     1021 ccatgcgtaa tgatgcaggc tggtgtgctc gtcgggctcg aatacgacat acagttcggc
     1081 tgtgccggaa aggaattcgt cgtagtcggc cgcgatcagc gaacccgcac ggtaggaacc
     1141 ggctccggtg tgccagtcgg tgcgcagctc gatcagcagc cagtcgcgat gcgccgacac
     1201 ggtggcgtct gtgggtgcat cgatgcggat gaactcgccg tcgcgcagct cgtggacctc
     1261 gtcgttgaag aagtcgacgg cgcggctcag cagcgtccgt tcgaagccgg gtgtgcggtc
     1321 cctcgacgcc gcgacgacca cgtcggtgcg tgccccgctg aagagggttt cggcgtcgtc
     1381 gagcggtgtg ccgcgtcgcc aacgtttgac cagccgtggg tacccggact cggtgagtga
     1441 gtcctcgccg aagtccgtgc cgatcagcac ggaattctcg tcctcccagc ttatctgggt
     1501 tttcgcctcg ggcacctgga agccgtcggc gacgaattcg cgtgtgcgca tgtcgaactc
     1561 acgcaccacg gcggcatccg agccgccccg cgacagtgtc accagcgcga gggagtggtc
     1621 gggctcgatg accttggcgc ccgcccatac ccagttgtcg tcctcctcgg cggccagcgc
     1681 gtcgaggtcg atcaccacct cccagtcggg tgtgtcggtg cgatagctgt ccagcctggt
     1741 gcggcgccac aggccccggg ggttggaggc gtcgcgccag aagttgtaca gccagtcgcc
     1801 gcggcggcgg acgtagggga ttcgcgcatc ggtgtcgagc acctcgaggg cctcggtgca
     1861 catctgctcg aaccgttcgc tgcccaactc ggcgagcgtg ggctcgttgt gcatgcggac
     1921 ccagtccagc gcgtcgtcgc cggtgatgtc ctcgagccag aggtatggat ccgtgcctgt
     1981 catcccgtca ttgtggccgc tgggtcgtca actgaggtga tggacctcct gcaggccgta
     2041 caccggagtt ggaatgccct cgtggcgtgc cttgagttgc agcgccagat agagcgagta
     2101 gtgccgtgac tggtgcaggt tgccgccgtg gaaccaaagg ttctcctgct gggtgggctt
     2161 ccacatgttg cgctgctcgc cctcccacgg cccggggtcc ttggcggtgt ccgagcccag
     2221 cccccacacc ttgcccacct tgtcggcgac gtcctgaccg atgaggtcgg ccgcccagcc
     2281 gttcatcgac ccgaag
//
LOCUS       DASYLX010000342         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_893130,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000342 DASYLX010000000
VERSION     DASYLX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893130"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..866)
                     /locus_tag="VGP27_08340"
     CDS             complement(3..866)
                     /locus_tag="VGP27_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV7851358.1"
                     /translation="MATVLMPIPDMDFDPTEVAVSWQVLSSHGHSIVFSTESGRPAVA
                     DEIMVSGRGLDIWSKLPGLSRVVVVGRFLRADRDGRAAYANMLGSTEYQHPAAWTGAR
                     LDDVDAVLLPGGHRARGMRSYIDSEILQGLVVDAFRRGMLVAAICHGVLLAARSVDPS
                     TGRSVLYGRETTALTWDLERRAWQIARLTRFWDPDYYRTYPERAGEPVGYMSVEAEVT
                     RALEKPSDFCNVPKDSPHRRKQTWGMVRDTATDPRPAFVVDDGTYISARWPGDTHTFA
                     SAVSAKLSEKP"
     gene            complement(892..1368)
                     /locus_tag="VGP27_08345"
     CDS             complement(892..1368)
                     /locus_tag="VGP27_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEV7851359.1"
                     /translation="MTLVNGPCLLRRAGLAGLLVALAPGCSFTPGQPAPAGHPTKVVV
                     EGQSQLITGQVTCVVGPTGEVNITVEDPEVTKSGAGAQPAIIVDLTFSNDTPYVSLLA
                     LNLPNIKLSIGQYREVAQPTVSRDGNSYKIIGQAAIADKPDQRSVYKPFEVDVTCP"
     gene            1498..2328
                     /locus_tag="VGP27_08350"
     CDS             1498..2328
                     /locus_tag="VGP27_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851360.1"
                     /translation="MRSPSVGRACYVAVLVCTAVVMWSFMYPTSATAEPRGEKPTIVL
                     VHGAWADTSSWNGEVDELQQAGYVVRAIANPLENLTTDSDHIANFLRTVDGPIVLVGH
                     SYGGSVITNAAEGNPNVTALVYVDAAAPDVGETNGSLSGADSVLKQKPESELFDKLPY
                     PNAPPAAVDLYLKKDIFLQHFGNDLPADVATRLWATQRVASTAAFDTASRNAAWKTIP
                     SWYFISSGDQIITPTSERAMAARAHSTVTEFDGGSHLTLISHPDAVTAVILDAVHSVT
                     "
     gene            complement(2261..>3061)
                     /locus_tag="VGP27_08355"
     CDS             complement(2261..>3061)
                     /locus_tag="VGP27_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7851361.1"
                     /translation="SAAAIALANSQRLKVGIGVLPVPMRNVALTAMEIATLDRAYPGR
                     IRVGVGHGVQDWMAQIGEKVASPMTLLREYVTVLSALLRGERVTHDGRYVKLADVGLD
                     WPPERNIELLLGAEGPRTLALSGELASGTVITGGTSPDRLREALRHISIGHAERGEPK
                     PHSTVVYLICATGPDPERRVRDELSYWQLDASQDLAVYGTAEEIAAGAKRWIDAGAET
                     IVFQPPAGADVDAFIDVIGRQVRPLIAMSPSEPHPVSPPSPRRGGISG"
BASE COUNT          526 a          988 c          991 g          556 t
ORIGIN      
        1 tctcatggct tttcgctgag cttcgccgac acggcgctgg cgaaggtatg ggtgtcaccc
       61 ggccaacggg cagagatgta agtgccgtcg tcgacgacga acgccggccg cgggtccgtc
      121 gcggtgtcgc gcaccatccc ccacgtctgc tttcgccggt gcggggagtc tttggggacg
      181 ttgcagaagt cgcttggttt ctccagcgcg cgagtcactt ccgcctcgac cgacatatag
      241 ccgacgggtt cacccgcccg ctccgggtat gttcggtagt agtcggggtc ccagaacctc
      301 gtgaggcggg cgatctgcca tgcgcgtcgt tccaaatccc aagtgagcgc agtggtttca
      361 cgaccgtaga gcaccgagcg gcctgtcgaa gggtcgacgg aacgggccgc gaggagaacg
      421 ccgtggcaga tcgccgcgac gagcatgccg cgcctgaacg cgtcgaccac gagcccctgc
      481 aggatctcgc tgtcgatgta gctgcgcatc ccgcgcgcgc ggtgaccgcc gggaagaagt
      541 acggcgtcga cgtcgtcgag tcgggcaccc gtccaggcgg cggggtgctg atattccgta
      601 ctacccagca tgttcgcgta cgcggcgcgg ccatcgcgat cggcgcgcag gaatcgcccg
      661 acgacgacca ctctgctcaa tcccggcagt ttcgaccaga tgtcgaggcc gcgtccggag
      721 accatgatct cgtcggcgac ggcaggccgg ccgctttcgg tgctgaacac gatcgagtgg
      781 ccatggctgc tgaggacctg ccagctgacg gcgacctcgg tggggtcgaa atccatgtcc
      841 gggatcggca tcaggacggt ggccacccta cgagcatgcc accggcgccg gctacggaca
      901 ggtgacgtcg acctcgaacg gcttgtagac cgaccgctgg tcgggcttgt cggcgatcgc
      961 ggcctggccg atgattttgt agctgttgcc atctctggag acggtgggct gagcgacttc
     1021 cctgtactgc cctatggaca gcttgatgtt gggcaggttg agggccagca gcgagacgta
     1081 gggcgtgtcg ttggagaagg tgaggtccac gatgatcgcg ggctgcgccc cagcgccgga
     1141 tttggtcact tccggatcct ccaccgtgat attcacctcg cccgtcggcc ccaccacaca
     1201 cgtgacctgt cccgtgatga gttggctttg tccttcgacg acgactttcg tggggtggcc
     1261 cgcgggtgct ggttggccgg gggtgaacga acaacccggg gccagcgcca ccagcaatcc
     1321 ggcgaggccg gcccgtcgta gaaggcaggg gccattcacg agtgtcattg tccttattgt
     1381 cgggctccgc tgccggatgc tgcgccctgt ttcatgaccg atcttggcgc tatccggtac
     1441 ggcgcgcggg cgttagcgtt gatgagtgat aaggaccctt ctcccaacgt gcttgccatg
     1501 aggtctccaa gcgtcggtcg cgcgtgctac gtcgcagtgt tggtgtgcac cgcagttgtc
     1561 atgtggtcgt tcatgtatcc gacttccgcc accgccgagc cgcgcggcga gaagcccacc
     1621 atcgtgctgg tgcacggcgc ttgggcggac acgtcgagct ggaacggaga agtcgacgag
     1681 cttcagcagg cggggtacgt ggtccgggcc atcgccaacc cattggaaaa cctcaccacc
     1741 gattcggatc acattgctaa cttcctgcga acggtcgatg gaccgatcgt tctggtcggc
     1801 cattcctacg gcggttcggt gatcacgaac gccgcggagg gcaaccccaa tgtcacggca
     1861 cttgtatacg tcgacgccgc cgcgcctgac gtcggtgaga ccaacggctc actcagcggc
     1921 gccgactcgg tgctgaaaca gaagcccgaa agcgaactgt tcgacaagct tccatatcca
     1981 aacgccccgc cggcagctgt ggacctgtac ttgaagaagg acatattcct ccagcacttc
     2041 ggcaacgacc tccctgccga tgtggcgacg cgattgtggg ccacccaacg tgtcgcttcc
     2101 acggccgcgt tcgacacggc gtcgcgcaac gcggcgtgga agacgatccc gtcgtggtac
     2161 ttcatcagca gcggcgacca gatcatcacg ccgacgtcgg aacgcgccat ggcggcgcga
     2221 gcccactcga ccgtcaccga gttcgacggc ggctcgcacc tcaccctgat atcccacccc
     2281 gacgcggtga cggcggtgat actggatgcg gttcactcgg tgacatagcg atcaacggcc
     2341 ggacctgtct tccgatgacg tcgatgaacg catcgacatc ggcgccggcg ggcggctgga
     2401 acacaatggt ctccgcaccg gcgtcgatcc accgcttcgc gccggctgcg atttcctccg
     2461 ccgtgccgta gacagccaga tcctgcgatg catccaactg ccagtaggac aactcgtcgc
     2521 gcacgcgacg ctcggggtcg ggccctgtcg cacagatcag gtacacaacg gttgaatgcg
     2581 gtttcggctc tcctcgttca gcatggccaa tactgatgtg tcgcagggct tcccgaagac
     2641 ggtccgggga ggtgccgcca gtgatcaccg ttcccgaggc cagttcgcca ctgagcgcca
     2701 gtgtccgcgg gccttcagcg cccaggagga gttcgatgtt ccgctccggc ggccagtcga
     2761 ggccgacgtc ggcgagcttc acatagcgac cgtcgtgggt gacccgctca ccgcgcagca
     2821 gtgcagagag gactgtcacg tactcgcgaa gcaaggtcat cggcgaagcc actttctcgc
     2881 cgatctgcgc catccagtcc tggacaccat ggccgacgcc gactcggatg cgcccggggt
     2941 atgcgcggtc aagagtggcg atctccattg cggtcagggc gacgttgcgc atcggtacgg
     3001 gcagcacacc gataccgacc ttgagcctct gactgttcgc cagcgcgatg gcggcagccg
     3061 a
//
LOCUS       DASYLX010000343         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2100033,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000343 DASYLX010000000
VERSION     DASYLX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100033"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGP27_08360"
     CDS             complement(<1..563)
                     /locus_tag="VGP27_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7851362.1"
                     /translation="MTDQHVAVAIVGGGPSGLTAAAALSGRVDGDVLVIEREVETGGI
                     PRHSDHPGYGMRDLKRFVSGPAYAKRLTAMAQNAGATLETEAMVTGWTGERRLQITSP
                     RGIRTVTADAVVLATGARERPRPARLVPGGRPDGVYTTGQLQNLVHLHHADVGSRALI
                     VGAELVSWSAVLTLREAGCATVGMVSG"
     gene            complement(560..1948)
                     /locus_tag="VGP27_08365"
     CDS             complement(560..1948)
                     /locus_tag="VGP27_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7851363.1"
                     /translation="MSGADVSDVIVVGAGIVGCAIARALAGTNLSVTLVEARGDVGDG
                     TSKANTALLHTGYDATPETLESRLVARGYELLGEYAEQTGIPVERTGALLVAWTDEEV
                     DALPGLKDKAERNFYHRCEIVSADEVYRRVPDLGPGVLAGLTVPGESIICTWTTNLAL
                     ATDAVARGARLLRGARVTGAVIGDGHTTLHTTRGDVSGRWVINAAGLGADYLDAEFGH
                     HRFTVTPRRGELLVFDKLTRPMVPCIVLAVPSSRGKGVLVSPTIYGNVMVGPTSESLE
                     DRTATETSEEGFEFLVSKGRALMPSLFDEEITATYAGLRAAIDRDDYLIDVDAQARYL
                     LVAGIRSTGLTSGMAIAEYVTGLLADAGLDVAERPDLPLPPTMPNLGDAYTRPFQDAE
                     RIAADPEYGRIVCFCERVTAGEIRDAFQSPIPPADLDGLRRRTRVMNGRCQGFYCGAH
                     TTALLDASGGTR"
     gene            complement(1945..3381)
                     /locus_tag="VGP27_08370"
     CDS             complement(1945..3381)
                     /locus_tag="VGP27_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV7851364.1"
                     /translation="MQHFLAIDQGTSATKAIVVDEAGQVASIAEVAVRPEYLAGGGVE
                     QDPEALFDSVVNAGRKALAQAGVPVSAVALANQGETVLAWDRDTGRPLTPAVVWQDRR
                     AESICAPLSASAEFIAQRTGLVLDPYFSAPKMAWIRANLTTAGVVTTTDTWLVHRLCG
                     AFVTDASTASRSLLISLDSVAWDDELTGLFGLTGEALPEIVASDQIIGSTDVFGPTIP
                     VAGLIVDQQAALLAQSCLAAGDAKCTFGTGAFLLAQLGGKAARSTSGLTTSVAWRLRE
                     QTSYCVDGQVYTAASAVRWAVDLGLVPAADQIDAVAADSSDGVLCVPALAGLAAPWWD
                     SAAAAAFTGMTLSSGRGQLVRALLEGIAAQVAALAGLVATDLGQSLTRLRVDGGLTQS
                     AVLMQAQADLARIPVDVYPSLHATALGAAACARLALDQSLGVAEVVGTWTPDHTYQPE
                     WSADRAAEFMARWMHGAQAVVAQNEPTT"
     gene            complement(3393..>4597)
                     /locus_tag="VGP27_08375"
     CDS             complement(3393..>4597)
                     /locus_tag="VGP27_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7851365.1"
                     /translation="ANPILGWILGWISFTFLAIVVVAVDYTIASTIFPALLNYEGTAT
                     IAWLVTGLVLLLQALLVGFSTPWAERVNNTFVSLELIGMVALTVLLLIVAAVRGETDF
                     SNLFSTGAIPADGYWSFGDLTTAGPWMLGFLLGAFTIVGFESAANLAEETNDPERVVP
                     RAMWQAVLASGVLGFLFIIAVTLAAGDPVALAESGTPIADVIDKTLGSAVATLLLVMV
                     VLAIFACGLVIMITGVRLTWAMSRDERFPGWQQWSQISPRFHTPLKATILYVVLAELI
                     LAIFSHSETALFTLFGAATLLPAVMYASTVVLYLIKRKSLPTSDKFNLGTWEIPILVV
                     AVVWLVFELALFRDSSFKQAWAYVIVMVVIGACYLGYLLATRGKRGLTMPGMASIDAE
                     LEAEAARE"
BASE COUNT          792 a         1586 c         1539 g          680 t
ORIGIN      
        1 tagccgctga ccattccgac tgtggcgcaa ccggcttcgc gcagggtcaa caccgccgac
       61 cagctcacca gctcggcgcc gacgatcaac gcgcggctgc cgacgtccgc gtggtgcaga
      121 tgcacgaggt tctgcaactg cccggtggtg tacacaccgt cgggacgccc gccgggtacc
      181 agccgcgcgg gcctgggccg ctcgcgcgcg ccggtcgcga gcaccacggc gtcggcggtg
      241 acggtgcgta tcccgcgcgg cgaggtgatc tgcagccgcc gctcaccggt ccagccggtg
      301 accatggcct cggtctccaa tgtcgcgccg gcattctgcg ccatcgcagt gagccgctta
      361 gcgtaggcgg ggccggagac gaatcgcttg agatcgcgca tcccgtagcc gggatgatcg
      421 ctgtggcgcg ggatcccgcc tgtctctact tcgcgttcga tgacgaggac atcaccgtcg
      481 acccggccgg agagcgcggc ggccgcggtc aatcccgatg ggccgcctcc gacgatcgcc
      541 accgctacat gctggtcggt catcgagttc caccgctcgc atcgagcagc gctgtggtgt
      601 gtgcgccgca gtagaagcct tggcagcggc cgttcatcac ccgcgtgcgg cgccgtaagc
      661 cgtcgagatc ggccggtggg atgggggatt ggaacgcgtc acggatctcg cccgcggtga
      721 cccgctcgca gaagcagacg atgcggccgt attcggggtc ggcggcgatg cgttcggcgt
      781 cctggaacgg gcgtgtgtag gcgtcgccga ggttgggcat cgtgggcggc aggggaaggt
      841 ccgggcgctc ggcgacgtcg aggcccgcat cggcgagcag accggtcacg tactctgcga
      901 tcgccatccc ggaagtcagc ccggtggaac gtatcccggc gacaagtagg tagcgggctt
      961 gcgcgtcgac gtcgatgaga tagtcgtcgc ggtcgatcgc ggcgcgtagc cccgcgtagg
     1021 tggcggtgat ctcctcgtcg aacagcgacg gcatcagcgc gcgacccttg gagacaagga
     1081 attcgaagcc ttcctccgaa gtctctgtgg ccgttcggtc ttccaggctc tcggaggtcg
     1141 ggccgaccat cacattgccg tagatggtcg ggctgacgag cactcccttg ccgcgcgacg
     1201 acggcacggc cagcacaatg cacggcacca tcggacgggt cagcttgtcg aacaccagta
     1261 gctcaccacg tcgcggggtg acggtgaaac ggtgatgtcc gaattcggcg tcgaggtagt
     1321 cggcgccgag gccggccgcg ttgatgaccc accgtccgga cacatcaccc cgggtggtgt
     1381 gcagtgtggt gtgcccgtcg ccgatgaccg caccggtgac gcgcgcgcca cgcaggagac
     1441 gtgcaccgcg cgcgacggca tcggtggcca gcgcgaggtt ggtggtccag gtgcagatga
     1501 tcgactcgcc gggcaccgtc agtccggcca ggactccggg gccgaggtcg gggacgcgtc
     1561 ggtacacctc gtcggcgctg acgatttcgc agcggtgata gaagtttcgt tcggccttgt
     1621 ccttcaggcc gggaagggcg tcgacctctt cgtcggtcca ggcgaccaga agcgcaccgg
     1681 tacgctcgac cgggatgccg gtctgctctg cgtactcgcc gagcaactcg tagccgcgcg
     1741 ccaccaggcg cgactcgagc gtttccggtg ttgcgtcgta gccggtgtgc agcagcgcgg
     1801 tgttggcctt gctggtgccg tcgcccacgt caccacgcgc ctccaccagg gtcaccgaga
     1861 ggttcgtgcc tgccagcgcc cgcgcgatgg cacaaccgac gatcccggcg ccgaccacga
     1921 tcacatcgct gacatcggcg ccactcatgt ggtcggctcg ttctgtgcca caacggcttg
     1981 cgccccgtgc atccatcgcg ccatgaattc ggcggcccga tcggcggacc actcgggttg
     2041 gtaggtgtgg tcgggtgtcc acgtgccgac cacctccgcc acgcccaggc tctggtcgag
     2101 cgccagtcgt gcgcacgcag cggcgcccag tgcggtcgcg tgcaacgacg ggtagacgtc
     2161 gacggggatg cgcgccagat cggcctgcgc ctgcatgagg accgcggact gggtgaggcc
     2221 gccgtcgacc cgcagccggg tcaacgattg gcccagatcg gtggcgacga gaccggcgag
     2281 ggccgcgacc tgtgcggcaa tgccctccag caacgcgcga accaactggc cgcgcccgct
     2341 gctcagcgtc atccccgtga acgccgccgc cgccgcggaa tcccaccacg gcgcggccag
     2401 cccggccagg gcgggcacac acagcacgcc gtcactggaa tcggctgcga cagcgtcgat
     2461 ctggtccgcc gccggcacca gtcccagatc gacggcccag cgcaccgcgg aagccgccgt
     2521 gtagacctga ccgtcgacgc agtacgacgt ctgctcgcgc aggcgccacg ccacggaggt
     2581 ggtcaggccc gacgtcgacc gcgccgcctt gccgccgagt tgggccaaca ggaaagcgcc
     2641 cgtgccgaac gtgcacttgg catcgcctgc agccaggcag ctttgggcca atagagcggc
     2701 ctgctggtcg acgatcaggc ccgcgaccgg aatcgtcggc ccgaaaacat ctgtgctgcc
     2761 gatgatctga tcggacgcga cgatctctgg cagcgcctcg ccggtcagcc cgaaaaggcc
     2821 ggtcaactcg tcgtcccacg cgacggagtc caacgagatc aacaacgacc ggctggccgt
     2881 cgaggcatcc gtgacgaacg cgccgcacaa ccggtgcacg agccaggtat cggtggtggt
     2941 gaccacgccc gccgtcgtca ggttcgcccg gatccatgcc atcttcggcg cagagaaata
     3001 cgggtcgagt accagcccgg tgcgctgcgc aatgaattcc gccgaagccg acagcggcgc
     3061 acagatcgac tcggcccggc ggtcctgcca caccaccgcg ggcgtcagcg gccgaccggt
     3121 gtcgcggtcc caggccaaca cggtctcgcc ctggttcgcc agcgccaccg cagacacagg
     3181 aacacccgcc tgcgccaagg ctttccggcc ggcgttgacg accgagtcga acagcgcctc
     3241 ggggtcctgc tcgacgccgc cgccggccag atactccggc cgcaccgcga cctcggcgat
     3301 cgaggcgacc tgcccggcct cgtcgaccac gatggccttc gtcgccgaag tcccctggtc
     3361 gatcgccagg aagtgctgca tcctcgcccc cgctattctc tggccgcttc agcctcgagc
     3421 tcggcgtcta tggacgccat tcccggcatc gtgagcccac gcttgccgcg ggtcgccagc
     3481 aggtagccga ggtagcacgc gccgatgacc accatcacga tgacgtatgc ccaggcctgc
     3541 ttgaaactcg agtcccggaa cagcgcaagc tcgaacacca gccacaccac ggcgaccacc
     3601 agaattggga tctcccaggt gcccaggttg aacttgtcgc tcgtcggcag cgacttccgc
     3661 ttgatcaggt agagcacgac cgtcgaggca tacatcaccg cgggtagcag cgtcgcggca
     3721 ccgaacaacg tgaacagggc cgtctccgag tgcgaaaaga tcgccaggat cagctcggcg
     3781 agaacgacgt acaaaatcgt ggccttcagc ggggtgtgga acctcgggga gatctggctc
     3841 cactgttgcc agcccgggaa gcgttcgtcg cgcgacatcg cccacgtcaa ccggacgccg
     3901 gtgatcataa tgaccagacc gcaggcgaag atcgccaaca ccaccatcac cagcaggagg
     3961 gtggcgactg ccgacccgag ggtcttgtcg atgacgtcgg cgatcggcgt gcccgactcg
     4021 gccagcgcga cggggtctcc cgcggccagc gtcacggcga tgatgaacag gaagccgagc
     4081 acaccggacg cgagaacggc ttgccacatc gcgcggggga caacccgctc ggggtcgttg
     4141 gtctcctcgg ccaggttcgc cgccgactcg aagccgacga tggtgaacgc gcccagcagg
     4201 aagcccagca tccacgggcc tgccgtggtc aggtcgccga agctccaata gccatcggcc
     4261 gggatcgcgc cggtgctgaa gagattcgag aagtccgtct cgccgcgtac ggcggccacg
     4321 atgagcagca gcaccgtcag cgccaccatg ccgatgagtt cgagcgagac gaacgtgttg
     4381 ttgacccgct ccgcccacgg cgtcgagaac ccgacgagca gggcctgaag cagcagcacg
     4441 agaccggtca ccagccacgc gatcgtcgcg gtgccttcgt agttcagcag tgcaggaaag
     4501 atcgtcgacg cgatggtgta gtccaccgcg accaccacga tggccaggaa ggtgaacgag
     4561 atccagccga gaatccaacc caagatgggg ttcgcca
//
LOCUS       DASYLX010000344         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3525453,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000344 DASYLX010000000
VERSION     DASYLX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525453"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VGP27_08380"
     CDS             <1..654
                     /locus_tag="VGP27_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7851366.1"
                     /translation="SIMPDDLLALCPTDARVVDTGPLTLDEIIGEIADAHASGLDVAR
                     LHSGDPSLYSALAEQCRRLAELGVDYEIVPGVPAFAAAAAALGRELTVPGVAQTVTLT
                     RVATLSTPMPPGEDLETLSAPGATLVLHLAAAQVDAIVPRLVAGGYRPETPCAVVAFA
                     SWPQQAVLRCTLAELADEMHTANITRTAVIVVGDVLTAESFADSYLYSTDRKRGGRH"
     gene            654..1379
                     /locus_tag="VGP27_08385"
     CDS             654..1379
                     /locus_tag="VGP27_08385"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HEV7851367.1"
                     /translation="MRVLLLGGTSEARTLAARLPARVDVISSLAGRVPDPALPPGEVR
                     IGGFGGVDGLRRWLEDSDVSAVVDATHPYAATITAHAAEVCSELAIPHVVLSRPAWPP
                     GGAILVDGDVAAAKAVQDNGFKRVFLTTGRSGVAVFRDVDAWFLIRAVTAPDPRTLPP
                     SRELVLSRGPYRYDDEFALLTEHRIECLVTKNSGGDMTRPKLDAAAALDVPVVMVNRP
                     MLPDGVTTVSTVDDAVAWVGSLG"
     gene            complement(1415..2479)
                     /locus_tag="VGP27_08390"
     CDS             complement(1415..2479)
                     /locus_tag="VGP27_08390"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV7851368.1"
                     /translation="MTIPRGPRDVTPAWLGSALGANVADVDVAPIGTGQTGATYRVAA
                     TYAADTDLPATFAVKLSAQDDAVRERVALGYRSEVEYYARVADRMSIPVPQSFYSEIS
                     DDGADFVLILADMAPAVQGDQIGGCSVEEAALAVEALAGLHGPSWCDPEWMALSGIAM
                     PKPGDDAAAGGLGEISVMAGGILVDKLGADISDEDKETLTTAMSLVTPWLKAEPHRYA
                     LMHGDYRLDNMLFDPGRTRITVVDWQTVGIGLPGRDLAYFTATSLDPDDRSEIEKDLV
                     AQYHRALLGYGITDYDFDTCWRDYRLGVVQAPLLTGLGFAFAASTERGDEMMLTMLHR
                     GCRAIRELETLELIRSYESV"
     gene            complement(2495..>3563)
                     /gene="cobJ"
                     /locus_tag="VGP27_08395"
     CDS             complement(2495..>3563)
                     /gene="cobJ"
                     /locus_tag="VGP27_08395"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEV7851369.1"
                     /translation="GVTSVSAASAATGTPLVQGDEVLTIIPGTLPVDELKRRLADTDA
                     AVVLKLGRSYPAVREALSSVGRLDEAYYVERASTPGQRVLPAADVDDDDDKVPYFSLA
                     MLPGAAARRAPAGRVAVVGLGPGDTEWMTPQSRRELAAATDLIGYGPYLSRVRPRDGQ
                     RLHPSDNTDEPARARLACALAEEGHAVAVVSSGDPGVFAMATAVLEEAKQWPGVEVRV
                     IPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAKADLVLAIYNPAS
                     RTRTWQVEAMRDLLLGHRDPGTPVVIGRDVSGPREEVKVVRLADLDPADVDMRCLLIV
                     GSSQTQWYDDRVFTPRRYPSL"
BASE COUNT          534 a         1191 c         1260 g          578 t
ORIGIN      
        1 tcgatcatgc ccgacgacct gcttgcgctg tgcccgaccg acgctcgggt ggtcgacacc
       61 gggccgctga cactcgacga gataatcggt gagatcgccg acgcgcatgc gtccgggttg
      121 gatgtggcgc gcttgcattc cggtgacccg tcgctctaca gcgcgctggc cgaacagtgc
      181 aggcgtctcg cggagctggg cgtggactac gagatcgtcc cgggcgtacc ggctttcgcg
      241 gcggcagcgg ccgcgctcgg acgtgagctg actgtgccgg gtgtcgcgca gaccgtgacg
      301 ttgacacgcg tcgcgacgct gtcgaccccg atgccgccgg gggaggacct cgagacgctg
      361 tcggcgcccg gtgcgaccct ggtgttgcat ctcgccgccg ctcaggtcga tgcgatcgtt
      421 ccgcgattgg tggcaggcgg ctaccgcccc gaaacgccct gtgcggtggt ggccttcgcg
      481 agctggccgc aacaggcggt gctgcggtgc acgctcgccg agctcgccga cgagatgcat
      541 accgcgaaca tcaccaggac cgcggtcatc gtcgtcggcg acgtgctcac cgcggagtct
      601 ttcgcggaca gctacctgta ctcgaccgac aggaaacgag ggggccggca ttgatgcgcg
      661 tgctgctcct cggaggtacc tcggaggccc gaacgctggc agcgcggtta cctgcccgcg
      721 tcgacgtgat cagctcgctg gccggccgcg taccagatcc cgcgctcccg cccggcgagg
      781 ttcgcatcgg tggcttcggc ggtgtcgacg ggttgcggcg atggctcgag gactcggatg
      841 tcagcgcggt cgtcgacgcc acccatccct atgccgcgac gatcacggcc cacgccgccg
      901 aggtgtgcag cgaacttgcg attccgcatg tcgtcctctc gcgaccggcg tggccgcccg
      961 gcggcgcgat cctggtcgac ggcgacgtcg ccgccgccaa ggccgtgcag gacaacgggt
     1021 tcaagcgcgt gttcctgacc acgggcaggt cgggtgtcgc ggtgttcagg gatgtcgacg
     1081 cgtggtttct gatccgcgcc gtcaccgcgc cggatccgcg gaccttgccg ccgagccgtg
     1141 aactggtcct ctcgcgcggg ccgtaccgct acgacgacga gttcgccctt ctcaccgagc
     1201 accgaatcga gtgcctggtg acgaagaaca gcggcgggga catgacaagg ccgaagctgg
     1261 acgcggctgc cgcactcgac gttccggtcg tgatggtgaa ccgtccgatg ctgcccgacg
     1321 gagtcaccac ggtgtcgacg gtcgacgacg cggtggcctg ggtcggctcg ctcggctgac
     1381 ggccgcggtc gtcagcgcgt cgtgcaaacc cggttcacac cgattcgtag gaccgaatca
     1441 actccagcgt ttccaactcg cggatggcgc ggcaaccgcg gtgcagcatg gtcagcatca
     1501 tctcgtcacc gcgctcggtg gacgcggcga acgcgaagcc caaacccgtg aggaggggcg
     1561 cctgcaccac gccgagtcgg tagtctcgcc agcaggtatc gaagtcgtaa tcggttatgc
     1621 cgtagccgag tagcgcgcga tggtattggg cgacgaggtc tttctcgatc tccgagcggt
     1681 catcgggatc gaggctcgtc gcagtgaagt acgcgaggtc tctgccgggc agaccgattc
     1741 cgaccgtctg ccagtcgacc accgtgatgc gagtgcgacc cgggtcgaac agcatgttgt
     1801 cgagccggta gtcgccgtgc atgagggcgt atcggtgggg ctcggctttc agccacggtg
     1861 tgaccaatga cattgcggtg gtgagggttt ccttgtcctc atcgctgatg tcggcgccga
     1921 gcttgtccac aaggattccg cctgccatca ccgagatctc gccgaggccg cccgccgcgg
     1981 cgtcgtcgcc gggtttgggc atcgcgatgc cggacaaggc catccactcg ggatcacacc
     2041 agctggggcc gtgaagtccg gcgagcgcct cgactgcaag ggccgcctcc tcgacgctgc
     2101 accccccgat ctgatcgccc tgcaccgcag gcgccatatc ggcgaggatg aggacgaagt
     2161 cggcgccgtc gtcggagatc tcggagtaga aggactgcgg gaccgggatg ctcatgcgat
     2221 cggcgacccg cgcgtagtac tcgacctcgg accggtagcc gagcgcgacc cgctcacgta
     2281 ccgcgtcgtc ctgggcggac agcttgaccg cgaatgtggc gggcaggtcg gtgtcggccg
     2341 catacgtcgc ggccacccgg taggtggcgc cggtctggcc ggtgccgatc ggcgcgacgt
     2401 cgacgtcggc gacgttggct cccagggcgg agccgagcca tgccggcgtt acatctcgag
     2461 gcccgcgtgg aatcgtcatg gctggcggac agtatcagag cgaggggtaa cggcgcgggg
     2521 tgaacacccg atcgtcgtac cactgggtct gcgacgatcc gacgatcagc aggcagcgca
     2581 tgtcgacgtc ggcggggtcg aggtcggcga gccgcaccac cttcacctcc tcgcgcggac
     2641 cggacacatc ccggccgatc accaccggag tgccggggtc acggtgtccc aacagcagat
     2701 cgcgcatcgc ttcgacctgc caggtgcggg tcctcgaggc ggggttgtag atagccagca
     2761 ccaagtcggc cttcgccgcg gcggtcagcc gtgcggcgat gacatcccag ggcttgagcc
     2821 gatcggacag cgagatcacc gcgtagtcgt ggcccagcgg tgcgccaacc cggctcgcga
     2881 ccgcctgcgc cgcggtcatc gcgggaatca cccggacttc gacgcccggc cattgtttgg
     2941 cttcctcgag gacggccgtg gccatcgcga agacaccggg atcgccggag gagacgaccg
     3001 cgacggcgtg tccctcctcg gcgagcgcgc acgccagtct cgcgcgcgcc ggttcgtcgg
     3061 tgttgtcgct tggatgcagg cgctgtccgt cacgcggccg gacgcgtgag aggtacgggc
     3121 cgtaaccgat gaggtcggtg gcggcggcga gctcgcggcg gctctgcggt gtcatccatt
     3181 cggtgtcacc ggggccgagg ccgaccacgg caaccctgcc cgcaggcgct cgccttgcgg
     3241 cggcgccggg gagcatggcg agcgagaagt acggcacctt gtcgtcgtcg tcgtcgacgt
     3301 cggccgccgg cagcacacgt tgcccaggtg tgctggcgcg ttccacgtag taggcctcgt
     3361 cgagccggcc caccgacgaa agcgcctccc ggacagcggg atacgagcgc ccgagtttca
     3421 ggaccacggc ggcgtcggtg tcggcgagcc tgcgcttgag ctcgtcgacg ggcagggtgc
     3481 cgggaatgat cgtcaacacc tcgtcgccct gcaccagggg tgtgcccgtt gcggccgatg
     3541 ccgcgctgac cgacgtcact ccc
//
LOCUS       DASYLX010000345         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1002145,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000345 DASYLX010000000
VERSION     DASYLX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002145"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VGP27_08400"
     CDS             <1..672
                     /locus_tag="VGP27_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307904.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEV7851370.1"
                     /translation="QFRPCLEAQVMDWADDVAYSVHDVEDGVVSGRIDMRVLADDDAA
                     AELGRLGESSGMGGGLRAEDLVSAAGRLSELPMVAAVGKYDATLAASVALKKLTSELV
                     GRFASAAIAATRAEAGPGPLVRYATDLRVPALVRAEVTLLKVLALQFIMSDPRHLDLQ
                     GRQRDRIHRVAEWLSAGAPATLDPIFVPAFNAAADDGERLRVVIDQIASFTEGRLERL
                     EPALD"
     gene            677..2611
                     /gene="dnaG"
                     /locus_tag="VGP27_08405"
     CDS             677..2611
                     /gene="dnaG"
                     /locus_tag="VGP27_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV7851371.1"
                     /translation="MAGSAGRSEATRGRGRIPDRDIAAIRERVQIEDVVGDYVQLRRA
                     GADSLKGLCPFHDEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKMEHVSFVEAVELL
                     ADRVGFTVTYTGAATSVQRDRGSRSRLIAANAAAAEFYAEALKSDEAAPARQYLLDRN
                     FDAETAARFGCGFAPSGWDTLTKHLLRKGFEFKELEAAGLSREGRRGPMDRFHRRLLW
                     PIRVSSGEVIGFGARRLFDDDQNQAKYVNTPETVLYKKSSVLFGLDHAKRDIAKAHQA
                     VVVEGYTDVMAMHQAGVTTAVASCGTAFGDEHLAMLRRLMMDDNFFRGELIYVFDGDA
                     AGRAAAVKALEGEQNLAGQSFVAVAADGMDPCDLRLKSGDGALRDLVARRTPLFEFAI
                     RTALADHDLDNAEGRVDALRRCVPMVARIKDPTLRDEYARQLAGWVGWNDVAQVIGRV
                     RDQSKGGKQTSAHGARAGRPAKAAPASVARPDPRDPTLWPQREALKSALQYAALAGPV
                     FDALTPETFTHPGYSAVRAAVAAAGGTSAGASGAEWIDAVRQQTGSDAAASLVNELGV
                     ESISVEDDEKLPRYISGVLARLQEVAVGRQIAEVKSKLQRMSPVEQGDEYHALFGDLV
                     AMEAYRRSLLEQASGDDLTA"
     gene            2693..2959
                     /locus_tag="VGP27_08410"
     CDS             2693..2959
                     /locus_tag="VGP27_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851372.1"
                     /translation="MIVLGAFAVMAAAGPAFAATTISTSAPQDYQALPPGCRSHFGNM
                     EDGICLDGAGGTPINIGTPFGQYGPNNYNNNYLLPGQTWTNGAN"
     gene            complement(2956..3207)
                     /locus_tag="VGP27_08415"
     CDS             complement(2956..3207)
                     /locus_tag="VGP27_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851373.1"
                     /translation="MFRYVAVLAVGYVLGTKAGRRRYEQIAGTYRAVTESPAAKAVIT
                     AGRRKIADRVSPDPEMVTLTPIDGDTAVLKPQTTAEKRR"
     gene            3355..3427
                     /locus_tag="VGP27_08420"
     tRNA            3355..3427
                     /locus_tag="VGP27_08420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3388..3390,aa:Asn,seq:gtt)
BASE COUNT          556 a         1093 c         1235 g          565 t
ORIGIN      
        1 cagttccgcc cgtgcctgga ggctcaggtg atggattggg ccgacgacgt ggcgtactcg
       61 gtgcacgacg tcgaggacgg agtcgtgtcg ggccggatcg acatgcgggt gctcgccgac
      121 gacgacgccg cggccgagct cggtcgactg ggggagtcga gcgggatggg cggggggctg
      181 cgcgccgagg acctggtgtc ggcggcgggg cggctgtccg agttgccgat ggtcgctgcc
      241 gtcggcaagt acgacgccac gttggccgcg tcggtagcgc tgaagaagct gaccagtgag
      301 ctggtcgggc ggttcgcgtc ggccgcgatc gcggccacca gggccgaggc agggccgggc
      361 ccgttggtcc gctatgccac cgatctgcgg gtaccggctc tcgtgcgcgc cgaggtgacg
      421 ctgctcaagg tgctggcgct gcagttcatc atgtccgatc ctcggcacct ggacctgcag
      481 ggccgccagc gtgaccggat ccaccgagtc gcggagtggt tgtcggccgg tgcgcccgcc
      541 acgctcgacc cgatcttcgt gcccgcgttc aacgccgcgg ccgacgacgg tgagcggctg
      601 cgggtggtca tcgaccagat agcgtcgttc accgaaggcc gattggagcg gttggaaccc
      661 gctctagact gattcggtgg ccggttcggc gggtaggagc gaagcgactc ggggtcgggg
      721 gaggatccct gatcgcgata tcgcggccat tcgtgaacgt gtgcagatcg aagacgtggt
      781 cggcgactat gtccagttgc gccgcgccgg ggccgactcg ctgaagggtc tgtgcccgtt
      841 ccacgacgag aagtccccgt cgtttcatgt gcggccgaac cacggccact tccactgctt
      901 cggctgtggc gagggcggtg acgtgtatgc gttcgtgcag aaaatggagc acgtcagctt
      961 cgtcgaggcg gtcgagctgc tggccgaccg ggtcggcttc accgtcacct acacgggcgc
     1021 agcgaccagc gtgcagcgcg accgcgggag tcgcagccgg ttgatcgccg ccaacgccgc
     1081 cgctgcggag ttctacgccg aggcgctgaa gtccgacgag gccgcgccgg cgcgccagta
     1141 tctgctcgac cgcaatttcg acgccgagac ggcggcgcgt ttcggctgtg gtttcgcgcc
     1201 gtcgggctgg gacacgttga cgaaacatct gctgcgcaag gggtttgagt tcaaggagct
     1261 cgaagccgcc gggctgtcac gggagggcag gcgcggcccg atggaccgct tccaccggcg
     1321 gctgctctgg cctattcggg tgtccagcgg cgaggtcatc ggattcggcg cccgcagact
     1381 tttcgacgac gaccagaacc aggcgaagta cgtcaacaca cccgagaccg tgctctacaa
     1441 gaagtcgtcg gtgctcttcg gcctcgacca tgccaagcgt gatatcgcca aggctcatca
     1501 ggcggtggtc gtcgagggct acaccgatgt gatggcgatg catcaggccg gggtgaccac
     1561 tgcggtggcg tcctgcggca ccgcattcgg agacgaacac ctggcgatgc tgcgacgact
     1621 catgatggac gacaacttct ttcgcggcga actgatctac gtgttcgacg gcgacgcggc
     1681 cggtcgggcc gccgcggtca aggcgctcga gggtgagcag aacctggccg ggcagtcgtt
     1741 cgtcgcggtg gcagccgacg ggatggaccc gtgcgatctg cggctgaaat ctggtgacgg
     1801 cgcactgcgc gatctggtgg cacgccgaac accgttgttc gagttcgcga ttcgcactgc
     1861 gctcgccgac cacgacctcg ataacgccga gggtcgggtc gacgcgctgc gccgttgcgt
     1921 gccgatggtg gcgcggatca aggacccgac attgcgcgac gaatatgcgc gccagctggc
     1981 cggctgggtg ggctggaacg acgtcgccca ggtgatcggc agagtccgcg accagtcgaa
     2041 gggcggcaag cagacctcgg cgcatggcgc acgggccggg cgtcccgcca aagcggcgcc
     2101 cgctagcgtg gcgcggcccg acccgcgcga tccgacgctg tggccgcagc gtgaggcctt
     2161 gaagtccgca ttgcagtacg ccgcgttggc ggggccggtg ttcgacgcgc tgacgccgga
     2221 gacgttcacc cacccgggat actcggcggt gcgagcggcc gtcgcggcgg cgggcggcac
     2281 atcggcgggc gcctcgggtg cggagtggat cgacgcggtg cgccagcaga cgggatcgga
     2341 cgcggcggcg agccttgtca acgagctcgg cgtggaatcg atcagcgtcg aggacgacga
     2401 gaaattgccg cgctacatca gcggagtgct ggcccgtctg caggaggtgg cggtcggacg
     2461 ccagatcgcc gaggtcaaat cgaagctgca gcggatgtcc ccggtggagc agggcgacga
     2521 gtaccacgcg ctgttcggcg acctggtcgc catggaggct tatcgccgca gcctgctgga
     2581 acaggccagc ggcgacgacc ttactgcgta accaggctcg acccgttatg gtgtagacgc
     2641 cctaggccca ctggtgaaag gcaatttggt ggcttcgaaa aagactcgtc ggatgatcgt
     2701 cctcggcgcc tttgcggtga tggccgctgc cgggccggct ttcgccgcga ccacgatttc
     2761 gacctctgcc ccccaggact accaggccct tccgcccggc tgccggtcgc acttcggcaa
     2821 catggaagac ggcatctgcc tggacggcgc tggcggtacg ccgatcaaca tcggcacgcc
     2881 ctttggccag tacggtccca acaattacaa caacaactac ctgctgccgg gccagacctg
     2941 gacgaacggc gccaactagc gccgtttctc ggccgtcgtc tgcggcttga gcaccgcggt
     3001 gtcgccatcg atcggcgtca acgtcaccat ctccgggtcc ggtgagacgc ggtcggcgat
     3061 tttacggcgt cccgccgtga tcacggcctt ggcggcagga ctttccgtga cggcgcgata
     3121 ggtgcccgcg atctgctcat atcggcgccg gccggctttg gtgcccaaca catacccgac
     3181 ggccaatacg gcgacatacc ggaacacagg gcccctccca aaagacggtg cgacggtgtc
     3241 catcctgcct cacgagtggg ggtgtgtgcc cggccgggag accattggcg gcgcgacccg
     3301 caggtaagct aaagtcaacc ctcggcccgc ggcagaccct cgcgggcaga gcaatcccct
     3361 gtagctcaat tggcagagca ttcggctgtt aaccgaaggg ttggtggttc gagtccaccc
     3421 gggggagcag gcatcacagc agccggctt
//
LOCUS       DASYLX010000346         8158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2208857,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000346 DASYLX010000000
VERSION     DASYLX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208857"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VGP27_08425"
     CDS             <1..1214
                     /locus_tag="VGP27_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEV7851374.1"
                     /translation="IPLLIGGATTSRAHTAVKVSPRRSGPVVWVKDASRSVPVAAALL
                     DDKQRPALLEATEKDYASLRERHAQKSERPMLTLEKARANRTPIEWDGYTPPVPAQGL
                     GVREFLDYDVAELREYIDWQPFFNAWEMKGRFPDILNNPVSGETARKLYDDAQEMLDT
                     LIKEKWLTANGVIGFFPANAVGDDIEVYTDETRTEVLTTLHNLRQQGEHRDGIPNRSL
                     GDYIAPKHTGLADHVGAFAVTAGLGSGDKIVEFKADNDDYSAILLESLADRLAEAFAE
                     RLHQRVRKEFWGFQPDERLDNEALIDEKYVGIRPAPGYPACPEHTEKATLWELMDVKE
                     RTGIELTESMAMWPGAAVSGWYFSHPQSQYFVVGRLAQDQVADYAKRKGWTLQEAERW
                     LAPNLGYNPED"
     gene            1251..1385
                     /locus_tag="VGP27_08430"
     CDS             1251..1385
                     /locus_tag="VGP27_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851375.1"
                     /translation="MRPNGFGSPSYAAAQPVAGCRRLHPEQPTDAFVLHHPEAKYFNV
                     "
     gene            complement(1382..2191)
                     /locus_tag="VGP27_08435"
     CDS             complement(1382..2191)
                     /locus_tag="VGP27_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7851376.1"
                     /translation="MSILVTGATGNIGRRVVDHLIDMGANDIRALTTDPAKANLPAGV
                     TPVIGYLGRPESLPAALAGVDRMYLAPLPATLQTTLEMAKVAGVAYVVALSGADYWQS
                     HADTIAASGVANTQLGPGEFLENLVLWADQIRTTATVRDPYPGVVEAMISMDDIARVA
                     ATLLMTPGQSHHGRMYELTGPESLIRAQIAEQIGVGIGVDVRFEQCSRDEAVRALRPV
                     MGDRAGWYLDTVAGALDKPQQANGLVAELTGVPAQSVAEWARENAALFGAV"
     gene            2232..2915
                     /locus_tag="VGP27_08440"
     CDS             2232..2915
                     /locus_tag="VGP27_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV7851377.1"
                     /translation="MKAVLWDMDGTLVDSEKVWDVSLAALYDKLGGELTPAVRASMVG
                     SVAENTMRIVYTDLGLDLDPAAMAESSRWLNEYTADLFEAGLPWCEGAKELLDALAAE
                     GTPMALVTNTQRALTERALNGIGRHYFSATVCGDEVANGKPAPDPYLRAAALLKLTPE
                     ECLAVEDSVTGTAAAERAGCPVLVVPNDVEVPDGPRRTHASSLAGLEVGDLRRIHARL
                     SGDVGERSA"
     gene            2998..4470
                     /locus_tag="VGP27_08445"
     CDS             2998..4470
                     /locus_tag="VGP27_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEV7851378.1"
                     /translation="MAGSNAPVGDESSLPIADEDFSSGRTAVRIASVAALGGLLFGYD
                     SAVINGAVDSIQEDFGIDNAKLGFAVASALLGAAVGAMSAGRIADRIGRISVMKIAAL
                     FFLVSAIGTALAPNVWLVVAFRVVGGIGVGVASVIAPAYIAETSPPSIRGRLGSLQQL
                     AIVSGIFLSFVINWVLQKIAGGPNEQLWLGLDAWRWMFMVMAVPAILYGSLAFTIPES
                     PRYLVASHKIPEARRVLTMLLGKRNLEITITRIQETLEREDKPSWKDLRKPTGGIYGI
                     VWVGLGLSVFQQFVGINVIFYYSNVLWQAVGFDADQSAVYTVITSVVNVATTLIAIAL
                     IDKVGRKPLLLIGSSGMAVTLITMAVIFANATIVDGKPSLHGASGVIALIAANLFVVA
                     FGMSWGPVVWVLLGEMFPNRIRAAALGLAAAGQWAANWLITVTFPGLRDHLGLAYGFY
                     GLCAVLSFLFVWRWVQETKGVSLEDMHGNIVHAGQSTAKT"
     gene            4507..4788
                     /locus_tag="VGP27_08450"
     CDS             4507..4788
                     /locus_tag="VGP27_08450"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEV7851379.1"
                     /translation="MKQSRAVKTFDALFAELSEKARTRPDGSGTVAALDGGVHGLGKK
                     ILEEAGEVWLAAEHEGDDALAEEISQLLYWTQVLMLSRGLSLDDVYGKL"
     gene            4797..5645
                     /gene="hisG"
                     /locus_tag="VGP27_08455"
     CDS             4797..5645
                     /gene="hisG"
                     /locus_tag="VGP27_08455"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV7851380.1"
                     /translation="MRVAVPNKGTLSEPAAEILSEAGYRRRTDQKDLTVIDPVNNVEF
                     FFLRPKDIAIYVGSGQLDFGITGRDLAAESDAPVRERLALGFGSSTFRYAAPAGRDWT
                     VADLAGRRIATAFPNLVRKDLAAKGIQATVIRLDGAVEISVALGVADAIADVVGSGRT
                     LGLHDLVAFGEPLCDSEAVLIEHTDPGDNNAAARDQLAARVQGVVFGQQYLMLDYDCP
                     RSLVEAATAVTPGLESPTIAPLADAAWVAVRALVPRRDVNAIMDELAAIGAKAILASD
                     IRFCRF"
     gene            5716..6216
                     /locus_tag="VGP27_08460"
     CDS             5716..6216
                     /locus_tag="VGP27_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEV7851381.1"
                     /translation="MTYFLVLLFALLIGVVAGLRALTAPAVVAWAGALGWINLDDTWA
                     HWVAHPITVTVLTILAIGELVTDQLPSTPSRRVPMQFGARLFTGAFSGAVLATGAVLN
                     PTAWVVVGAIGAALIGAVIGTLGGAEARKRLVAASGGRDLPVALVEDAIAIVGGFAVV
                     ALVSVI"
     gene            6217..7602
                     /locus_tag="VGP27_08465"
     CDS             6217..7602
                     /locus_tag="VGP27_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV7851382.1"
                     /translation="MATAAQRFDAIIIGAGQAGPPLAGRLTEAGQTVAVIERKLVGGT
                     CVNTGCIPTKTLVASAHAAQTARRGAEYGVGIGDVSVDMAKVKARKDKIMLDDRRGVE
                     SWLEGMDNCNLIRGHGRFVDPHTIRVDDQVLQADRIFLNVGGRAVIPEMPGLSDIDYM
                     TNVGILDLDTVPEHLVIIGGSYIALEFAQMYRRFGARVTVVEKGPRLTSREDEDVSAT
                     IKEILEKEGIAVELNATAIAFHKRDNGFDVTPRDGAGPIAGTHLLVATGRRPNTDDLH
                     LQNAGVETDGRGYIVVDDHCRTNVEHIWAMGDCNGRGAFTHTSYNDFEIVAGNLLDDD
                     PRRISDRVPTYALYIDPPLGRAGLTVDQVRKSGRKALIGTRPMTRVGRAVEKGETQGF
                     MKVVVDAETEEILGAAILGVGGDEVVHSLLDIMSAGDPYTSIARTMHIHPTVSELVPT
                     MLQAMKPLDQG"
     gene            complement(7547..7786)
                     /locus_tag="VGP27_08470"
                     /pseudo
     CDS             complement(7547..7786)
                     /locus_tag="VGP27_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
     gene            complement(7810..>8158)
                     /locus_tag="VGP27_08475"
     CDS             complement(7810..>8158)
                     /locus_tag="VGP27_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7851383.1"
                     /translation="AISWRRQRDLDNGSPVVWMSPLAHLVDAEPMTPVQRLALVVDCA
                     NGAGAAIDPAAFVFMNTDTAMHLHRVPIGNDFALRARGSIGPDGIGATTADIFDRQGF
                     VGTCAQTLFVQRR"
BASE COUNT         1385 a         2615 c         2742 g         1416 t
ORIGIN      
        1 agatcccgct gctgatcggt ggcgcgacca cctcgcgcgc ccacaccgcg gtgaaggtgt
       61 cgccgcgtcg ttccggtccg gtggtctggg tcaaggacgc gtcccgctcg gtgccggtgg
      121 ctgctgcgct cctcgacgac aagcagcgtc cagcgctgct ggaggccacc gagaaggact
      181 acgcatccct gcgcgaacgg cacgcccaga agagcgagcg gccgatgctg acgctggaga
      241 aagcccgcgc caaccggacg ccgatcgagt gggacggcta cacgccaccg gtgcctgccc
      301 agggtcttgg cgtgcgcgaa ttcctggact acgacgtcgc cgagttgcgc gagtacatcg
      361 actggcagcc gttcttcaac gcctgggaga tgaagggcag gttccccgac atcctcaaca
      421 accctgtctc gggcgagacg gcccgcaagc tgtacgacga cgcccaggag atgctcgaca
      481 ccctgatcaa ggagaagtgg ctgactgcca acggcgtgat cgggttcttc ccggcgaacg
      541 cggtcggcga cgacatcgag gtctacaccg acgagacccg taccgaggtg ctgaccacgc
      601 tgcacaacct gcgccagcag ggcgagcacc gggacggcat cccgaaccgg agcctcggcg
      661 actacatcgc gcccaagcac acgggtctgg ccgaccacgt cggtgccttc gccgtcaccg
      721 ccggactcgg cagcggagac aagatcgtcg agttcaaggc cgacaacgac gactacagcg
      781 cgatcctgct ggagtcgctc gccgaccggc tggcagaggc gttcgccgaa cggctgcacc
      841 aacgggtccg caaggagttc tggggatttc agcctgacga gcggctggat aacgaggcgc
      901 tgatcgatga gaagtacgtg ggaatccgcc ccgctcccgg ttatccggca tgcccggagc
      961 acaccgagaa ggcgacgctc tgggagctga tggacgtcaa ggagcggacc ggcatcgagc
     1021 tgaccgagtc gatggcgatg tggcccggtg ccgccgtcag cggctggtac ttctcgcacc
     1081 cgcagtcgca gtacttcgtg gtcggccggc tggcccagga ccaggtcgcc gactatgcga
     1141 agcgcaaggg ctggaccttg caggaggccg agcgctggct tgcccccaac ctcggctaca
     1201 acccggagga ctgagctgtg acggcgtcat gctcaccgag ccgaaacacc atgcggccga
     1261 acggtttcgg ttcaccttcc tatgcggcgg cgcagccggt tgccgggtgt cgccgactcc
     1321 atccggagca gccgaccgat gcgttcgtcc tgcaccaccc cgaggccaag tacttcaacg
     1381 tctagaccgc gccgaagagc gcggcgtttt cgcgcgccca ttcggcgacc gactgcgcgg
     1441 gaacaccggt gagctcggcg accaacccat tggcctgctg cggtttgtcg agcgcaccgg
     1501 ccaccgtgtc gaggtaccag ccggctcggt cgcccatcac cggccgcagg gcgcggacgg
     1561 cctcgtcgcg gctgcactgt tcgaacctca catcgacgcc gattccgact ccgatctgct
     1621 cggcgatctg tgcgcggatc agcgactccg ggccggtgag ctcatacatc cgaccgtgat
     1681 gcgattggcc gggcgtcatc agcagcgtcg cggccaccct ggcgatgtca tccatcgaaa
     1741 tcatcgcttc caccaccccc ggatacggat cgcgcaccgt cgccgtcgtc ctgatctggt
     1801 ctgcccagag caccaggttc tccaggaatt ctcccggccc gagctgcgtg ttggcgacgc
     1861 ccgaggccgc gatggtgtcg gcatggctct gccagtagtc ggcaccggac agcgccacga
     1921 cgtaggccac gccggcgacc ttggccatct cgagcgtggt ctgaagcgtt gcgggcagcg
     1981 gcgcaaggta catccggtcg acgccggcaa gcgcagcggg aagcgattcg ggtctgccga
     2041 ggtatccgat gacgggtgtc acgccggctg gcagattcgc cttggccggg tcagtggtca
     2101 gtgcgcggat gtcgttggcg cccatgtcga tcagatgatc gacgacgcgc ctgccgatgt
     2161 tgccggtggc gccggtcaca aggatgctca tgattttcac gctacgtgaa ctccgtgaca
     2221 caatcgcggg tgtgaaggcg gtgctgtggg acatggatgg caccctcgtc gactccgaga
     2281 aggtctggga cgtatccctg gctgcgctct acgacaagct cggcggtgag ctgacgcccg
     2341 ccgttcgggc atcgatggtg ggcagcgttg ccgagaacac catgcgcatc gtctacaccg
     2401 acctcggtct cgacctcgat cccgccgcga tggccgagtc cagccgctgg ctgaacgaat
     2461 acaccgccga cctcttcgag gcgggcctgc cgtggtgtga gggcgccaag gagctgttgg
     2521 acgcgcttgc cgccgagggc acaccgatgg cgctggtcac caacacgcag cgcgccctca
     2581 ccgagagggc actgaacggc attgggcgcc attacttctc cgccacggta tgcggagacg
     2641 aggttgccaa cggcaaacct gcacccgacc catatcttcg cgcagcggcg ttgctgaagt
     2701 tgacgccgga ggagtgcctg gcagtcgagg actcggtcac cgggacggcg gcggcggagc
     2761 gcgccggatg cccggtgttg gtggtaccca acgatgtcga ggtgcccgac ggtccgcggc
     2821 ggactcatgc gagttcgctg gcgggcctgg aagtcgggga tcttcgccgg atccatgccc
     2881 gactcagcgg tgacgtcggg gaacgctcgg cgtaagtgca cccgcggcgt gcttgccaat
     2941 ggtaagacag gggtattcaa gtgacccgga tcacgcaacg ctgcgaaagg gacgcccatg
     3001 gcaggttcca atgcacccgt aggggatgaa tcgtcgttac cgatcgccga cgaggacttc
     3061 tcatcgggcc gaaccgctgt caggatcgcc tcggtggccg ccctcggcgg tctgttgttc
     3121 ggctacgaca gcgccgtgat caacggtgcg gttgattcga ttcaggaaga cttcgggatc
     3181 gacaacgcga agctggggtt cgccgtggcg tccgcgctgc tgggtgccgc agtcggcgcg
     3241 atgtctgccg gccggatcgc cgaccggatc gggcgcatct cggtgatgaa gatcgcggcc
     3301 ttgttcttcc tggtgagtgc gatcggcacg gcgctggcgc cgaacgtgtg gctggtggtc
     3361 gccttccggg tggtcggtgg catcggggta ggtgttgcct cggtcatcgc ccccgcgtac
     3421 atcgccgaga catcgccgcc cagcatccgc gggcgtcttg gctcgcttca gcagctggcc
     3481 atcgtgtccg gcatcttctt gtccttcgtg atcaactggg tgctgcagaa gatcgcgggt
     3541 ggcccgaacg agcagctgtg gctggggttg gatgcgtggc gctggatgtt catggtgatg
     3601 gctgtgccgg caattctgta cggatcgctg gcattcacca ttcccgagtc tccgcgctat
     3661 ctggttgcca gccataagat tccggaagct cgtcgcgtgc tcaccatgct gctcggtaag
     3721 cggaatctgg agatcaccat cacccgtatc caggaaacgc tggaacggga ggacaagcct
     3781 tcctggaaag atctgcgcaa gccgaccggc ggcatctacg gcatagtctg ggtcggcctc
     3841 gggctgtcgg tattccagca gttcgtcggt atcaacgtga tcttctatta ctccaacgtg
     3901 ctgtggcagg cggtcggatt cgacgccgac cagtcggccg tatacacggt catcacctcg
     3961 gtggtgaatg tggcgaccac attgatcgcc atcgcactga tcgacaaggt cggacgcaag
     4021 ccgctgttgc tcatcggatc gtccgggatg gcggtgaccc tgataacgat ggcggtcatc
     4081 ttcgccaacg ccacgatcgt cgacggcaaa ccgagcctgc acggtgcgtc gggtgtgatc
     4141 gcgctgatcg ccgcgaacct cttcgtcgtc gccttcggca tgtcgtgggg accggtggtc
     4201 tgggtgctgc tcggcgagat gttccccaac cgcatccgcg cggctgccct gggcttggcc
     4261 gccgccggtc agtgggccgc gaactggttg atcaccgtca cgttcccggg cctgcgcgat
     4321 cacctcggct tggcctacgg cttctacgga ctgtgcgcgg tgctctcgtt cctcttcgtg
     4381 tggaggtggg tgcaggagac caagggtgtc tcgctggagg acatgcacgg caacatcgtc
     4441 cacgcggggc agtcgaccgc gaagacgtag acggcgctaa accgccgtga ctttgcccgg
     4501 gagcccgtga aacaatcgcg agccgtgaag actttcgacg cgctgttcgc cgagctcagc
     4561 gaaaaggcgc gcacccggcc ggacggtagc ggcaccgtcg ccgcgctgga cggcggcgtg
     4621 cacgggctgg gcaagaagat cctcgaagag gcgggcgagg tgtggctggc cgcagagcac
     4681 gagggtgacg acgcgctggc cgaggagatc agccagttgc tgtactggac gcaggtgctg
     4741 atgctctccc gcggactgag cctcgacgac gtgtacggaa aactgtgagc gacacgctgc
     4801 gggtcgcggt acccaacaag ggcacgctga gcgagcccgc cgccgagatt ctgtcggagg
     4861 ccggctaccg acgtcgcacc gaccagaagg acctgacggt catcgacccg gtcaacaacg
     4921 tcgagttctt cttcctgcgc cccaaggaca tcgccatcta cgtaggatcg gggcagctcg
     4981 atttcggtat caccggtcgc gatctggccg ccgaatccga tgccccagtg cgcgagcggc
     5041 tggccctggg cttcgggtcg tcgaccttcc gatacgccgc ccctgcgggc cgtgactgga
     5101 cagtcgccga cctcgcaggc aggcggatcg ccaccgcgtt tcccaacctg gtccgaaagg
     5161 atctggcggc caaagggatc caggcgactg ttatccgcct cgacggagcc gtcgagatct
     5221 ccgtcgcact cggtgtggcc gacgccatcg ccgacgtcgt cggctcaggt cgcaccttgg
     5281 gcctccatga tctggtcgcc ttcggtgagc cgctgtgtga ttcggaggcg gtgctcatcg
     5341 aacacaccga ccccggcgac aacaacgccg cggcacgcga ccaattggcc gcgcgcgtgc
     5401 agggcgtggt gttcggccag cagtatctga tgctggacta cgactgcccg cggagcttgg
     5461 tcgaggcggc caccgccgtc actccgggac tggagtcgcc gaccatcgcg ccgctggccg
     5521 atgcggcctg ggtggcagtg cgcgcactgg taccgcgccg cgacgtgaac gcgatcatgg
     5581 acgagttggc ggccatcggc gccaaggcaa tactggcctc cgacatcagg ttctgccgct
     5641 tctgactaag tcgggttctg tccagctctg cgtgttagcg tccgggtgac gttttcaccg
     5701 gtctgagagg tccgcatgac gtatttcctc gttctgctgt ttgctctgct gatcggtgtg
     5761 gtcgccggtc tgcgcgcatt gaccgcgccc gcggtggtgg catgggccgg agcgctcggc
     5821 tggatcaacc tcgacgacac ctgggcgcat tgggtggcgc acccgatcac ggtcacggtg
     5881 ctgaccattc tggcgatcgg ggagttggtc accgaccaac tgccgagcac accgagcaga
     5941 cgggtgccga tgcagttcgg cgccaggctg ttcaccggcg cgttctcggg tgcggtgctc
     6001 gcgaccgggg ccgtgctgaa cccgacggcg tgggtcgtcg tcggtgcgat cggtgccgcg
     6061 ttgatcggcg cggtcatcgg cacgctcggc ggtgccgaag cgcgtaagcg gctcgtcgcg
     6121 gccagtggcg gacgcgacct gccggtcgcc cttgtcgagg atgcgatcgc cattgtcggc
     6181 ggattcgcgg tcgtcgcact cgtctcggtc atctgaatgg caactgcggc acagaggttc
     6241 gatgcgatca tcatcggtgc cggccaggcc gggccacccc tggcgggcag gctgaccgag
     6301 gccgggcaga cggttgcggt gatcgaacgc aagctcgtcg gcggtacttg cgtcaacacc
     6361 ggctgcatcc cgaccaagac gctcgtggcc agtgcccacg ccgcgcaaac cgcccgccgc
     6421 ggcgccgaat acggcgtcgg cataggcgat gtcagcgtgg acatggccaa ggtcaaggcg
     6481 cgtaaggaca agatcatgct cgacgaccgc aggggcgtcg agtcctggct cgagggcatg
     6541 gacaactgca acctgatccg cggacacggt cgttttgtcg acccgcatac cattcgggtt
     6601 gacgaccagg tcctgcaggc ggacaggatc tttctgaatg tcggtggccg ggctgtgatc
     6661 ccggagatgc cgggactttc cgatatcgac tacatgacca atgtcgggat cctcgatctc
     6721 gacacggtgc ccgaacatct cgtcatcatc ggtggcagct atatcgcgct cgagttcgcg
     6781 cagatgtacc ggcgcttcgg tgcccgggtc accgtcgtcg agaaaggtcc gcggctgaca
     6841 tcccgcgagg acgaggacgt ctctgcgacc atcaaggaga tcctcgagaa ggaggggatc
     6901 gccgtcgaac tcaacgctac ggcgatcgcg tttcacaagc gggacaacgg tttcgacgtg
     6961 acgccgcgcg acggtgccgg ccccatcgca ggcacccatc tgctggtggc gaccgggcgg
     7021 cggccgaaca ccgacgacct gcacctgcaa aatgcgggcg ttgaaaccga cggccgcggc
     7081 tacatcgtgg tcgacgacca ttgccggaca aacgtcgaac acatctgggc gatgggcgac
     7141 tgcaacggtc gcggcgcgtt cacgcacacg tcgtacaacg acttcgagat cgtggccggc
     7201 aatctgctcg acgacgatcc gcgccggatc agcgaccgcg tgccgaccta cgcgctctac
     7261 atcgacccgc cactcggccg cgcaggcctg accgtcgacc aggtgcgcaa gtcaggccgt
     7321 aaggcgttga taggcacgcg accgatgacg agagtcggac gtgcggtcga aaaaggggag
     7381 acacaggggt tcatgaaggt tgtcgtcgac gccgagaccg aggagatact cggtgccgcc
     7441 atcctcggcg tcggcgggga cgaggtcgtg cattcactcc tcgacatcat gtccgccggg
     7501 gatccctaca cttcgatcgc ccgcacgatg cacatccacc cgacggtcag tgaattggtg
     7561 ccgacgatgc tgcaggcgat gaagccgctc gaccaaggtt gagttgtcgc gtcaccgccg
     7621 ccacccgccg gccgagatga ttgcacgtcc ggacatcgga gggatgcatc tgatccgggc
     7681 gggcgtcgac gtcggtctgc gcgcacgcgc ccaatcccag gttgacccag tgcatgtggt
     7741 gctgggctgc gaacaccgac ggcgaggtca gggtgtgcat tttgtcgcac catgtgccgt
     7801 ttatgtatgt cagcgccgct gaacgaacaa ggtctgcgca cacgtcccga cgaacccttg
     7861 ccgatcgaag atgtctgctg tggtggcgcc gatgccgtcg ggccctatcg agccccgtgc
     7921 ccgcagcgcg aagtcattgc cgatgggaac gcggtgcaga tgcatcgcgg tatcggtgtt
     7981 catgaacacg aaggccgcgg gatcgatcgc cgcgcccgcg ccgttggcgc agtcgaccac
     8041 cagggcaagg cgctgcaccg gcgtcatcgg ttcggcatcc acgagatggg ccagtggact
     8101 catccacacg accggtgatc cgttgtcgag atcccgttgt cggcgccagc tgatcgct
//
LOCUS       DASYLX010000347         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2647378,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000347 DASYLX010000000
VERSION     DASYLX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647378"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..797
                     /gene="cofC"
                     /locus_tag="VGP27_08480"
     CDS             144..797
                     /gene="cofC"
                     /locus_tag="VGP27_08480"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV7851384.1"
                     /translation="MSGTQTGAPADVGLVIAVKRLTAAKTRLAPVFSAATRERVVLAM
                     MVDTIRAALAVPALMTVTVVTPDRVAAEAAQEMGAQVLTDPTPDGHRNPLNNAIAAAE
                     AAVSESTPNVVALQGDLPALQPQELAEAISAARGYRRSFVGDRHGTGTSALFAFGVPL
                     DPHFGADSARRHQHSGAIELTGAWPGLRCDIDTPDDLLVARRLGVGAATAQAIGRPR"
     gene            863..3040
                     /locus_tag="VGP27_08485"
     CDS             863..3040
                     /locus_tag="VGP27_08485"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEV7851385.1"
                     /translation="MTEAETRIRADGTDAVPSESASAREGDQKTPEAPPAATAPAVDD
                     ALPEDRYLNRELSWLDFNARVLALAADTSLPLLERAKFLAIFSSNLDEFYMVRVAGLK
                     RRDEMGLSVRSADGLSPREQLRRISERTQQIANRHAHVFLDSVRPALADEGILIVNWL
                     QLDDDERGRLSTYFHEQVFPVLTPLAVDPAHPFPFVSGLSLNLAITVKAPDDGGQHFA
                     RIKVPDNVDRFVELDSRDGALRFLPMEELIAAFLPVLFPGLEIVEHHAFRITRNADFE
                     VEEDRDEDLLQALERELARRRFGSPVRLEVADDMTENMLELLLRELDVNPGDVIEVPG
                     LLDLSSLWQIYGVDRPDLKDPPFVPATPPAFGERETPKSIFATLRDGDVLVHHPYDSF
                     STTVQRFIEQAAADPNVLAIKQTLYRTSGDSPIVNALIDAAEAGKQVVALVEIKARFD
                     EQANIKWARALEQAGVHVVYGLIGLKTHCKTALVVRREGSAIRRYCHVGTGNYNPKTA
                     RLYEDVGLLTAAPDIGADLTDLFNSLTGYSRKDSYRNLLVAPHGVRRGIIERIEREAA
                     ATRDGAEGRIRVKANALVDEQVIDALYRASQAGVRVEVVVRGICALRPGAPGFSENIT
                     VRSILGRFLEHSRIIHFGVSDEFWIGSADMMHRNLDRRVEVMAQVKDPRLKTQLDEVF
                     ESAMNPATRCWELGDDGSWTASPREGETVRDHQVWLMERHRHP"
     gene            3077..>3469
                     /locus_tag="VGP27_08490"
     CDS             3077..>3469
                     /locus_tag="VGP27_08490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7851386.1"
                     /translation="MAKGSSNSAAAPSKKAVLAAGAVLWRCSDDGDEPEVAVIHRPRY
                     DDWSLPKGKVDVGESEPITAVREVAEETGYRSRLGRRLASVSYPVEEQCIKKVRYWAA
                     RIVDGEFTPNPEVDELKWLPVSAAIKQVQ"
BASE COUNT          633 a         1125 c         1156 g          555 t
ORIGIN      
        1 gcatcggcca acaccttggc cagcgctgtt ccccacgcac cggcccccat caccgccgct
       61 tcgaccacgg tttcacccta tcgcggtccg ctgtggcgac atctgggctg cgcccgcggc
      121 ccggactggc aggatgagaa agcatgagcg gcacgcagac cggcgcgccg gctgacgtcg
      181 ggttggtcat cgcggtcaag cggctcaccg ccgccaagac gagactggca ccggtgttct
      241 ccgctgcgac cagggaacga gtggtgctgg cgatgatggt cgacaccatc agggccgcgc
      301 tggcggtccc cgcgctgatg accgtcaccg tcgtcacacc cgaccgggtt gccgccgagg
      361 ccgcgcagga gatgggcgcg caggtactga ccgatccgac accggacggc caccgcaacc
      421 cgctgaacaa cgcgatcgcc gccgcggaag ccgccgtcag cgagtccacg ccgaatgtcg
      481 ttgcactgca aggagatcta cccgcgcttc agccgcagga actggccgaa gcgatcagcg
      541 ccgcccgcgg ctaccgccgc agtttcgtcg gcgaccggca cggcactggc acctcggcac
      601 tgtttgcctt cggtgtaccg ctggaccctc acttcggggc cgactcggcc cgacggcatc
      661 agcattcggg agccatcgag ctgaccggag cctggccggg cttgcgctgc gacatcgaca
      721 cgcccgacga cctgctggtg gcacgccggc tcggtgtggg tgcggcgacc gcgcaggcca
      781 tcgggcgacc aaggtaaaca gctgtcgacg gaaaacggtc gtcggtagaa gttcggctcg
      841 cggatgagga atgatcgaag gaatgaccga agccgagaca cgaattcgag cggatggcac
      901 cgatgcggtg ccgagtgaat cggcgtccgc acgcgagggg gaccaaaaga ccccggaggc
      961 accacctgcg gcgacggcac cggccgtcga cgatgcgctg cccgaggatc ggtacctcaa
     1021 ccgcgagctg agctggctgg acttcaacgc ccgggtgctg gcgctggccg ccgacacgtc
     1081 gctgcccctg ctcgagcggg cgaagtttct cgcgattttc tcgtcgaacc tcgacgagtt
     1141 ctacatggtc cgggtggcag ggctgaaacg tcgcgacgag atgggtctgt cggtgcgctc
     1201 cgcggacggc ctgtcgcccc gcgaacagct gcggcgcatc agtgaacgca cgcagcagat
     1261 cgccaacaga cacgcgcacg tattcctcga ttcggtgcgc ccggcgctcg ccgacgaggg
     1321 aatcctgatc gtcaattggc tacagctcga cgacgacgag cggggcaggc tgtccaccta
     1381 cttccacgag caggtgttcc cggtgctgac accgctggcg gtcgacccgg cgcatccgtt
     1441 cccgttcgtc agcgggctga gcctgaacct ggccatcacc gtcaaggcgc ccgacgacgg
     1501 cggtcagcac ttcgcccgaa tcaaggtgcc cgacaacgtc gaccgtttcg tcgaactcga
     1561 cagccgcgac ggtgcgttgc gtttcctgcc gatggaggaa ctgatcgcgg cgtttctgcc
     1621 ggtgctgttc cccggcttgg agatcgtcga gcaccacgcg ttccgtatca ctcgcaacgc
     1681 ggatttcgag gtcgaagaag accgcgacga ggatctgctg caggcgctcg agcgggagct
     1741 ggcccgcagg cgattcggct cgccggtgcg cctcgaagtc gccgacgaca tgaccgagaa
     1801 catgttggaa ctcctgctgc gcgagctcga cgtgaacccc ggggacgtca tcgaagtacc
     1861 cggcctactg gatctctcgt cgctgtggca gatctacggc gtcgaccgtc cggacctcaa
     1921 ggaccccccg ttcgtgccag ccaccccgcc cgccttcggc gagcgggaga cacccaaaag
     1981 catcttcgcg acgctgcgcg acggtgacgt cctcgttcac cacccgtacg actcgttctc
     2041 gacaaccgtg cagcggttca tcgaacaagc agccgccgac ccgaatgtgc tggcgatcaa
     2101 gcagacgttg taccgcacgt cgggtgactc tccgatcgtc aatgcgttga tcgacgccgc
     2161 tgaggccggt aagcaggtcg tcgcactcgt ggagatcaag gcgcgattcg acgaacaggc
     2221 caacatcaag tgggcgcgag cacttgagca ggcgggcgtg catgtcgtgt acggattgat
     2281 cgggctgaag acgcactgca agacggcact ggtggtgcgc cgcgaaggct cggcgatccg
     2341 gcgctactgc catgtgggca ccggcaacta caacccgaaa accgcgcggc tgtatgagga
     2401 tgtcgggctg ttgacggccg cccccgacat cggcgccgac ctcaccgatc tcttcaactc
     2461 gttgaccggc tactcacgca aggattccta ccgcaatctg ctggtcgcgc cgcacggcgt
     2521 gcgcaggggc atcatcgaac gaatcgaacg cgaggccgcg gccacgcgag acggcgccga
     2581 gggcaggatc cgcgtgaagg ccaacgcgtt ggtcgacgag caggtgatcg acgcgctcta
     2641 tcgcgcctca caggccggcg tgcgagtcga ggtggtggtc cgcggtatct gcgcgctgcg
     2701 gcccggcgct cccggtttct ccgagaacat cacggtgcga tcgattctcg gcaggttcct
     2761 cgaacactca cgaattattc acttcggcgt cagcgacgag ttctggatcg gcagtgccga
     2821 catgatgcat cgtaatctcg accgtcgggt cgaagtgatg gcgcaagtga aggatccgag
     2881 actgaagacg caactggacg aggtgttcga gtcggcgatg aatccggcga cccggtgctg
     2941 ggaactcggc gacgacggga gttggacggc gtcacctcgg gagggcgaga cagtgcgtga
     3001 tcatcaggtg tggctgatgg aacgtcaccg gcacccttga ggttcccgcg gttgtcgatg
     3061 atctgcagga gttgaggtgg cgaagggttc ctccaacagc gccgcggcgc cgtccaagaa
     3121 ggccgttctg gccgcgggtg cggtgctctg gcgttgcagc gacgacggcg acgaacccga
     3181 agtcgcggtg attcaccgcc cgcgttacga cgactggtcc ctgcccaagg gcaaggtcga
     3241 cgtcggagag agcgaaccca tcaccgcggt acgcgaagtg gccgaggaaa cgggttaccg
     3301 ttcacgcctc gggcggcggt tggcgtcggt cagctatccg gtcgaagagc agtgcatcaa
     3361 gaaggtgcgg tactgggcgg cacggatcgt cgacggcgag ttcaccccga acccagaagt
     3421 cgacgagctg aagtggctgc ccgtttctgc ggcgatcaag caagtccaa
//
LOCUS       DASYLX010000348         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1331150,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000348 DASYLX010000000
VERSION     DASYLX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331150"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2334)
                     /gene="gyrA"
                     /locus_tag="VGP27_08495"
     CDS             complement(<1..2334)
                     /gene="gyrA"
                     /locus_tag="VGP27_08495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV7851387.1"
                     /translation="MTDTTLPPGDEAGDRIEPVDIQQEMQRSYIDYAMSVIVGRALPE
                     VRDGLKPVHRRVLYAMFDSGFRPDRSHAKSARSVAETMGNYHPHGDSSIYDTLVRMAQ
                     PWSLRYPLVDGQGNFGSPGNDPPAAMRYTEARLTPLAMEMLREIDEETVDFIPNYDGR
                     VQEPTVLPSRFPNLLANGSGGIAVGMATNMPPHNLRELAEAVYWCLENHDADEEATLA
                     AVTQCVKGPDFPTKGLIVGSQGIHDTYATGRGSIRMRGVCEIEEDNRGRTGIVITELP
                     YQVNHDNFITSIAEQVRDGKLTGISNIEDQSSDRVGLRIVVELKRDAVAKVVLNNLYK
                     HTQLQTSFGANMLSIVDGVPRTLRLDQLIRHYVNHQLDVIVRRTTYRLRKANERAHIL
                     RGLVKALDALDEVIALIRASQTVDVARQGLIELLDIDDIQAQAILDMQLRRLAALERQ
                     RIVDDLAKIEAEIADLEDILAKPERQRAIVHDELAEIIEKYGDDRLTRIVPADGEVSD
                     EDLIAREDVVVTITETGYAKRTKTDLYRSQKRGGKGVQGAGLKQDDIVNHFFVCSTHD
                     WILFFTTQGRVYRAKAYELPEASRTARGQHVANLLAFQPEERIAQVIQIKGYEDAQYL
                     VLATRNGLVKKSKLTDFDSNRSGGIVAVNLRNGDELVGAVLCSAEDDLLLVSAKGQSI
                     RFSATDEALRPMGRATSGVQGMRFNTDDALLSLNVVQPDTFLLVATAGGYAKRTAIDE
                     YPVQGRGGKGVLTIQFDKRRGSLVGALVVDDETELYAI"
     gene            complement(2356..>3996)
                     /locus_tag="VGP27_08500"
     CDS             complement(2356..>3996)
                     /locus_tag="VGP27_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEV7851388.1"
                     /translation="ALSTRLEVDISRDGHEWFQSYDHAVPGTLKEGDATKKTGTTVRY
                     WADPNIFETTNYDFETVARRLQEMAFLNKGLTITLADERVTPEEVVDEVVSDTAEAPK
                     SADEKAAEARAPHKTKNRTFHYPGGLVDYVKHINRTKTAIHQSIVDFSGKGEGHEVDV
                     AMQWNAGYSESVHTFANTINTHEGGTHEEGFRAALTSVVNKYAKDKKLLKDKDPNLTG
                     DDIREGLAAVISVKVAEPQFEGQTKTKLGNTEVKSFVQRICNEQVSHWFEANPAEAKI
                     VVNKAVSSAQARMAARKARELVRRKSATDIGGLPGKLADCRSTDPRKSELYVVEGDSA
                     GGSAKSGRDSMFQAILPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDLAK
                     LRYHKVVLMADADVDGQHISTLLLTLLFRFMKPLVENGHIFLAQPPLYKLKWQRAEPE
                     FAYSDRERDGLLEAGKKAGKRINVDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQIT
                     LDDAAAADELFSILMGEDVEARRSFITRNAKDVRFLDV"
BASE COUNT          585 a         1276 c         1301 g          834 t
ORIGIN      
        1 gatggcgtag agctcggttt cgtcatcgac caccagcgct ccaacaagac tgccacgtcg
       61 cttgtcgaac tggatggtca gcaccccttt gccaccgcgg ccctgaaccg ggtactcgtc
      121 gatcgcggtg cgtttcgcat agccgccggc ggtagcgacc agcaggaatg tgtccggctg
      181 cacgacgttc aacgacagca gcgcgtcgtc ggtgttgaac cgcatcccct gcacaccaga
      241 ggtggcccgg cccatcggcc gcagcgcctc atcggttgct gagaagcgga tcgactgacc
      301 ctttgccgac accagcagca ggtcgtcctc ggccgagcac agcacggcgc ccaccagctc
      361 gtcgccgttg cgcaggttca ccgcgacgat gccgcccgaa cggttcgagt cgaagtcggt
      421 gagtttcgac ttcttcacca ggccgttgcg agtcgccagc accaggtact gcgcgtcctc
      481 gtaacccttg atctggatga cctgggcgat ccgctcctcg ggctggaacg ccagcaggtt
      541 ggcgacgtgc tggccgcgtg ccgtgcgcga cgcctccggc agttcgtagg ccttggcccg
      601 gtacacccgg ccctgtgtgg tgaagaacag aatccagtcg tgggtcgagc agacgaagaa
      661 gtggttgacg atgtcgtcct gcttcaggcc ggcgccctgc acgcccttgc cgccgcgctt
      721 ctgactgcgg tacaagtcgg tcttggtgcg cttggcgtag ccggtctcgg tgatcgtgac
      781 gacgacgtcc tcgcgggcga tcaggtcttc gtcgctgacc tcgccgtcgg cggggacgat
      841 cctggtgagc cggtcgtcgc cgtacttctc gatgatttcg gccaattcgt cgtgcacgat
      901 ggcgcgctgg cgctccggct tggccaggat gtcttccaga tcggcgatct cggcctcgat
      961 cttggccagg tcgtcgacga tgcgctggcg ttccagagcg gccagccggc gcagctgcat
     1021 gtccaggatt gcctgggcct ggatgtcgtc gatgtccagc aattcgatca ggccctggcg
     1081 cgcgacatcg acggtttgcg acgcccggat cagggcgatg acctcgtcga gcgcgtcaag
     1141 cgccttgacc agcccgcgca gaatgtgggc gcgttcgttc gccttgcgca gccggtaggt
     1201 ggtgcgccgc acgatgacat cgagttggtg gttgacgtag tgccggatca gctggtcgag
     1261 ccgcagtgtg cgcggtacgc cgtcgacgat ggacagcatg ttggcgccga agctggtctg
     1321 cagctgggtg tgcttgtaca ggttgttcag caccactttg gccaccgcgt cgcgcttgag
     1381 ctccacgacg atgcgcaggc cgacgcggtc gctgctctga tcctcgatgt tcgagatgcc
     1441 ggtcagcttg ccgtcgcgca cctgctcggc gatcgaggtg atgaagttgt cgtggttgac
     1501 ctggtacggc agttcggtga tgacgatgcc ggtgcgccct cggttgtcct cttcgatctc
     1561 gcagacaccg cgcatcctga tcgacccgcg gcccgtggcg taggtgtcgt ggatgccctg
     1621 cgagccgacg atcaatccct tggtggggaa gtcggggccc ttgacgcact gggtgacggc
     1681 ggccagcgtc gcttcctcgt cggcatcatg gttctccagg caccagtaca ccgcctccgc
     1741 gagctcgcgg agattgtgcg gaggcatgtt ggtcgccata ccgaccgcga tcccgccgga
     1801 gccgttggcc agcaggttcg ggaagcggct gggcaggacc gtcggctcct gcacccgccc
     1861 gtcgtagttc gggatgaaat cgactgtctc ctcgtcgatt tcgcgcagca tttccattgc
     1921 caacggcgtg agacgcgcct cggtgtacct catggcggcc ggcgggtcgt tgcccggcga
     1981 gccgaagttg ccctgcccgt cgaccaatgg gtagcgcaac gaccacggct gggccatccg
     2041 gaccagggtg tcgtagatcg acgagtcgcc gtgcgggtgg tagttgccca tcgtttcggc
     2101 aacggagcgt gcggatttcg cgtggctgcg gtccggccgg aaacccgagt cgaacatcgc
     2161 gtagagcacc cggcggtgta cgggcttgag cccgtcgcgc acctccggca gcgcgcggcc
     2221 gacgatcaca ctcatcgcgt agtcgatgta gctgcgctgc atctcctgct gaatgtcgac
     2281 cggctcgatg cggtctcctg cttcgtcgcc gggcggcaac gtggtgtcag tcattgattt
     2341 cagtcctatt gattgttaaa cgtcaaggaa gcgaacgtct ttggcattac gggtgatgaa
     2401 gctgcgccga gcctcgacgt cctcgcccat caggatcgag aacagctcgt cggcggccgc
     2461 cgcgtcgtcg agggtgattt ggcgaagcac ccgcaccgac ggatccatcg tggtctccca
     2521 cagctccttg gcgtccattt cgcccagacc cttgtatcgc tggatgccgt cgtcgacgtt
     2581 tatcctcttg cccgccttct tgccggcctc gagcagcccg tcgcgctcgc ggtcggagta
     2641 ggcgaactcc ggttcggcgc gctgccattt cagcttgtac agcggcggct gcgccaggaa
     2701 gatgtggccg ttttcgacca gcggcttcat gaaccggaac agcagcgtca gcagcagcgt
     2761 cgagatgtgc tggccgtcga cgtcggcgtc ggccatcagc acgaccttgt gataccgcag
     2821 cttggcgagg tcgaactcgt cgtggatacc ggtgcccagc gcggtgatga tcgcctgcac
     2881 ttcggtgttc ttgagcacac ggtcgatgcg ggccttctcg acgttgatga tcttgccgcg
     2941 caacggcagg atcgcctgga acatcgagtc gcggccgctc ttggccgagc cgcccgcgga
     3001 gtcgccctcc accacgtaca gctcggactt gcgtgggtcg gtggatcggc agtcggccaa
     3061 cttgccgggc agtccgccga tgtcggtggc gctcttgcgc cgcaccagct cgcgtgcctt
     3121 gcgcgccgcc atgcgggcct gcgccgacga aaccgccttg ttcacaacaa ttttggcctc
     3181 agccgggttg gcctcgaacc agtggctcac ctgctcgttg cagatccttt gaacgaacga
     3241 cttcacctcg gtattgccga gcttggtctt ggtctgaccc tcgaattgag gctcagccac
     3301 cttcaccgag atgacggctg ccagcccctc gcggatgtcg tcacccgtca ggttcggatc
     3361 cttgtccttg agcagcttct tgtccttggc gtatttgttg acgacgctgg tcaatgccgc
     3421 ccggaagccc tcttcgtggg tgccaccctc gtgggtgttg atcgtgttgg cgaaggtgtg
     3481 caccgactcc gagtagccgg cgttccactg catcgcgacg tcgacctcgt ggccttcacc
     3541 cttgccgctg aagtccacga tgctctgatg gatcgcggtc ttggtccggt tgatgtgctt
     3601 gacgtagtcg accaggccgc cggggtagtg gaacgtgcgg ttcttggtct tgtgcggcgc
     3661 cctcgcctcg gccgccttct catccgccga cttgggtgct tcggcggtat cgctgaccac
     3721 ctcgtcgacg acttcctcgg gggtcacccg ctcgtcggcg agtgtgatgg tcagaccctt
     3781 attgaggaaa gccatctcct gcagccggcg ggccacggtc tcgaagtcat agttggtggt
     3841 ctcgaagatg ttgggatccg cccagtacct gacggtggtg cccgtcttct tggtcgcgtc
     3901 gccctctttc agcgtgcccg gcacggcgtg gtcgtaggac tggaaccact cgtggccgtc
     3961 tcgggagatg tcgacttcca gccgggtgga cagggc
//
LOCUS       DASYLX010000349        14075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2647451,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000349 DASYLX010000000
VERSION     DASYLX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647451"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VGP27_08505"
     CDS             <1..826
                     /locus_tag="VGP27_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEV7851389.1"
                     /translation="STMRRGNMSPTIARGEMALWKLYLVDAQYGSLGYDGFHAWEILE
                     GRYTLAVLFEYAGTLGLLDLDYVHPTGARQDFQENWGGDNLATLSRYDGLQAIRLTAL
                     GRYALGLTDTYQPPVGKAEAQLLKVLPTLDVVATGILSAADRLVLSAYAEHTADRVWT
                     ISAASLLAAINTGRDPQDFVTFLAQRSEHELPGSLTTLIGDVTRRTTQLTDLGHARVI
                     ECADPALAALLTRDRALRHLCRPLGDRHLAIPLDQELTFRKALLKLGYVLPGQRRP"
     gene            complement(979..1341)
                     /locus_tag="VGP27_08510"
     CDS             complement(979..1341)
                     /locus_tag="VGP27_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEV7851390.1"
                     /translation="MARKVETTLVDDMTGAAADETVSFGLDGVEFEIDLSANNAGVLR
                     DILADYANAGRKVGKLTTVSNSRSRPSATPRGNREQTAAIRAWAREAGRQVSDRGRIS
                     AEVAKAFEDAHRGRAAVG"
     gene            complement(2206..3687)
                     /locus_tag="VGP27_08515"
     CDS             complement(2206..3687)
                     /locus_tag="VGP27_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEV7851391.1"
                     /translation="MSVAAPLLFAFDNSYVRELEGLYEPWQATPAPAPRLLALNEELA
                     TELGVDADALRTPDGVAVLVGNTTPDGASPVAQAYAGHQFGGFVPRLGDGRALLLGEV
                     FDVEGRRRDLHLKGSGRTPFARGGDGKAPVGPMLREYAIGEAMHALGIPTTRALAVVA
                     TGDHVARDTVLPGAVLTRVAASHVRVGTFQYAAVHNGPTLVRRLADYVIARHHPNAVE
                     AENPYLAFFESVVDAQASLVAGWMLVGFIHGVMNTDNMTISGETIDYGPCAFMDAFDP
                     ATVFSSIDHGGRYAYGNQPRIAQRNLARLAETLLPLFHVEADTALAAATDVLQSFPDR
                     YDEYWSRGMHAKLGVADAQRSDGALIDDLLALLHAQSVDFTSCFRSLSSSLRGDAAPA
                     RLLFAEPSAFDAWADRWRAQLSSQASDPRAIAEAMDRVNPVYIPRNHQMEEALAAASA
                     GDLGPFRRLLDVLSQPFDERPGLELYAAPAPPSFGAYRTFCGT"
     gene            3780..4151
                     /locus_tag="VGP27_08520"
     CDS             3780..4151
                     /locus_tag="VGP27_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEV7851392.1"
                     /translation="MDELDARLHRIGQHHFRAKFRLSGRDRAVVDLRGITTVRRHAEE
                     LVGRRLAPAEPRNDGRQTPYRGHPVFVAQHATATCCRTCLARWHDIPAGHALDDAERG
                     YVVEVICRWIAGQCTPHHPPP"
     gene            complement(4308..5027)
                     /locus_tag="VGP27_08525"
     CDS             complement(4308..5027)
                     /locus_tag="VGP27_08525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851393.1"
                     /translation="MAARQKHLFREWQVGDGREQALADYVTTHARAGDLDDAIRVVDE
                     FCYRKSFLINVGDEKGKILDAAVRRVRPRVLLELGTYCGYSALRMARAMPPDAHLCSI
                     EFNAANAAIARRIWEHAGIADRVTAVVGTLGDGEATLWRLREEHGFFPGGVDLVFIDH
                     AKKEYVPDLERILDEGWLHPGSVVVADNVKFPGAPEYRAYMHARDGTGWRTTEHSTHA
                     EYQSLVRDLVLESEYLQGTSS"
     gene            complement(5077..5220)
                     /locus_tag="VGP27_08530"
     CDS             complement(5077..5220)
                     /locus_tag="VGP27_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851394.1"
                     /translation="MKQLSRTFVVSAAAAGAALAMATRRNALRVPRWVVVGRGCRWGR
                     GCD"
     gene            5705..7390
                     /locus_tag="VGP27_08535"
     CDS             5705..7390
                     /locus_tag="VGP27_08535"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7851395.1"
                     /translation="MLLVVLVCGCEATPAPPTGSAEWVERLRQFEEGYFARNPPFAVQ
                     KGRHEFDGRLPDWSATGIRATVQWLHAQRTQAEAFDQNALSAEQRFERQYLLAQIDTD
                     LFWLEDAEQPFTNPAYYLDSGLDPNVYLTRPYASAETRLKAFIGYARAVPAAAAQIRV
                     NLRTPLPRSFIKYGVDSFGGFPDFYRKDAPKAFAEVKDPTLQAELASTIEPAARAMQD
                     LTDWLNSGLPQATENFALGPVKFADMLRMTERVTTPVATLESVGRADLARNLVALKQA
                     CAEYLPGAAIEACVAKVEADKPTGGAVEGARAQLGMLRQFVVDHGLVGIPGTEQALVA
                     EAPPYQRSNFAYIDIPGPYDKGMPSVYYIAPPDPSWPKADQEAYVPGKSVLLFTSAHE
                     VWPGHFVQFLHANRSPFDFGRLFVGYAYAEGWAHYTEEMMWEAGLGNGAPEAHIGQLS
                     EALLRNVRFLCAIGMHTQGMSVASCETLFREQAYQDPGSARQQAARGSYDPAYLNYTM
                     GKLMIRKLRADWTANRGGRAAWRAFHDGFLSYGGPPIPLVREQMLGTAGGDLF"
     gene            7685..8404
                     /locus_tag="VGP27_08540"
     CDS             7685..8404
                     /locus_tag="VGP27_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539738.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV7851396.1"
                     /translation="MKRGLSGVVVLALGAVALAGCSGGSEPAAATSTSAGLTPGITAR
                     GLGTVTKIPDTVTIVIGVQTRAQSAKAGLEANTDKATALINVLKSKGVTPADLQTSQL
                     SVNPTSDSATGRITGYEVTNQVTAKLHDIGAAGGLIDAAGDVAGDAVRLQQLSFSIGD
                     DNAARAQARANAVRQAQAHAKQMADGAGVRLGQIRSITEVPASPPGPLGRDAAAAAPV
                     PIEPGTQDLTVMVEVVYAIDQ"
     gene            8578..9225
                     /locus_tag="VGP27_08545"
     CDS             8578..9225
                     /locus_tag="VGP27_08545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851397.1"
                     /translation="MASTPERPTPALSVFDDAYATHSAPWVIGEPQPAIVTLEREGWV
                     RGKVLDVGCGTGEHTIYLSALRYDVYGMDSSLRAVEQARANAAARNVEAHFQVADALA
                     LGDDTTYDTVIDSALFHIFDADDRARYVRSLHLACRPGALVHVLALSDTGPAFGPQVS
                     DATIREAFSQRWVLEDLRLTEYRGAIVRDEHAVALHRQIGEFVDLPAWLARARRI"
     gene            9335..9748
                     /locus_tag="VGP27_08550"
     CDS             9335..9748
                     /locus_tag="VGP27_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851398.1"
                     /translation="MLLDGINHIAWISKDVARLGRFYADVFDADVGPTRTHGKDGKET
                     MTIIRIGPHTELNIFVIEGNTEPERQTPMWGRGRIDHVGLQAASPEAFATIRQRLIEK
                     AASDGTINDFGAVHSIFFRDPDGLEGEVLIAKAST"
     gene            10184..11506
                     /locus_tag="VGP27_08555"
     CDS             10184..11506
                     /locus_tag="VGP27_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851399.1"
                     /translation="MSLGGAGISFAREDELAPVIDGRVIAFGIPGASAVSPIGTFLPG
                     GPIHDNPTLAAFTQPGRVLDPIRIIVGSRSNFGAPIANPDQAAGALLSIDPGEAELAV
                     PATFASSGDQASALGGAVQMYSAQSPTWRNGFYNPNAVTADQTAVSNPIGLSINNAFG
                     RLWPANAPFGLDGPGSSTIDDPDGRPLKGAPNTRTGGVYFGDLTGRQPAQIIPGALAK
                     AAVGTAFLGRSPDGSGRAVFCVVVADGSIVQEHTGKALDGLAPAGIVGPLSGGSRAEE
                     RGERRFTPRLGAIINYTPVLILYVSQPFDDSIKAINLEIGGPAGNEVFVPTTSRAIRS
                     QTLDIPVDLAPVHIESEDPNWSSNTTMESDSDFYVCNAGNDTIVRMRQDGAAVARCRV
                     WARGRPLGHARLNGIATSPDGAKIWVTYVGVLPGSDDHDGGVLELPAF"
     gene            complement(11644..12201)
                     /locus_tag="VGP27_08560"
     CDS             complement(11644..12201)
                     /locus_tag="VGP27_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7851400.1"
                     /translation="MTVRADAQRNRQRLLAAALRAFSSSEEKVTLEAIAKCAGVGIGT
                     LYRHFPTREALVEAVYRNELEQLRVSAEDLLTSRRPDVALRTWMDRFADYVAAKRGMA
                     EALRAVVASGAISSSETRDVLTAAIRSLLDAAVVAGTVRFDVRADDVLASLTGVFLAA
                     GAPQQRDQAGRMLDLLMDGLRARTG"
     gene            12316..13053
                     /locus_tag="VGP27_08565"
     CDS             12316..13053
                     /locus_tag="VGP27_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851401.1"
                     /translation="MSGVEGKVVAITGASSGIGEATALLLARRGAKVVLGARRAERLE
                     ALATRVTAAGGEAAFARVDVRRRDDLRRLVGLAGDQYGKLDVLVSNAGIGPISPLDDL
                     RVEEWEQMIDINVKGVLYGIAAALPVFRRQGFGHFVHTASTAAHRITPNQSVYAATKT
                     AVRVISEGLRQEAGDRLRVTVISPGFTRTDFADDVTNPQVHAQIAESKDLIAMPSDAV
                     ARAIAFAIEQPADIDMGEIVVRPTAQS"
     gene            13498..>14075
                     /locus_tag="VGP27_08570"
     CDS             13498..>14075
                     /locus_tag="VGP27_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851402.1"
                     /translation="MTDATPASGVPTVLLVHGAFADGSGWAGVIERLQALGIRVRALV
                     NPLRGLASDSAYVASAIEQTPGPVLAVGHSYGGAVITNAAAQANNVVGLVYVAAFAPE
                     EGETLLDIEGSSRDSVLSSALREARYPIGQGSETAVELFIDLASFHEVFAGDLPAAQA
                     AVLGAAQRPVAAAAFGEKSGPPAWKNLPAWAVV"
BASE COUNT         2493 a         4725 c         4527 g         2330 t
ORIGIN      
        1 ttcgaccatg cgacggggca acatgagccc caccatcgcc cgcggtgaga tggcgctgtg
       61 gaagctctat ctcgttgacg cccagtacgg cagtctgggc tacgacggct tccatgcgtg
      121 ggagatcctc gaaggccgct acacccttgc ggtgctgttc gaatacgccg gcacgctggg
      181 actgcttgac ctcgactacg tccacccaac cggagcgcga caggacttcc aggagaactg
      241 gggcggtgac aatctagcca cgttgagcag gtacgatggc ctccaggcga tccgactcac
      301 cgcactgggt cgctacgccc tcggcctgac cgacacctac cagccacccg tcggcaaggc
      361 ggaagcccag ctgctgaagg tccttcccac tctggatgtc gtcgccaccg gcatcctgtc
      421 cgcagctgat cggcttgtgc tgtcggccta cgccgagcac accgccgacc gagtctggac
      481 catctccgcc gcaagtctgc tggccgcgat caacaccggt cgcgaccccc aggatttcgt
      541 caccttcttg gcccagcgca gcgagcacga gctgcccggc tcactgacga ccctcatcgg
      601 cgatgtcacc cgtcgtacca cgcagctgac cgacctcggc cacgcccgcg tgatcgaatg
      661 cgccgacccc gcgctggccg cgctcctcac ccgcgaccgc gcacttcgtc atctttgccg
      721 ccccctcggc gaccgccatc tcgccattcc actcgaccaa gagctgacgt tccgtaaggc
      781 actgctgaag ctcggctatg tcctgccggg acagcgcagg ccctgagcga cgtgaccacg
      841 ccgtgatgcc ggtttgcggg ggtcggggaa gggcagactc gccggtgagt ttactccgcg
      901 aggggcggag aaatgcgcca gtccccaccc ctcgcggaga tacaacgccg atcgggtgcg
      961 ccgtgtcgca cccgaccgtc accccacggc cgcccgtcca cggtgtgcgt cctcgaacgc
     1021 cttcgctacc tcagcggaga tccggccacg gtccgacacc tggcggccag cttcgcgggc
     1081 ccatgcgcgg atcgccgcgg tctgctcccg attgccgcgc ggagttgcgg acggacgaga
     1141 gcgactgttc gacacggtgg tgagcttgcc gaccttgcgt cccgcgttcg cgtagtccgc
     1201 gagaatgtcc cgcagaacac ccgcgttgtt tgccgaaagg tcgatctcaa actcgacgcc
     1261 atccaggccg aagctcaccg tctcatcagc ggcggcgccc gtcatgtcat caacgagtgt
     1321 ggtctctacc ttgcgcgcca tgaaggtctc ctaagtcgag aggattgtct gacacaatga
     1381 tagaacaact gtcaccaaaa cgctaagcag ggtgtgcccc tgcccggaag ggcaaccgca
     1441 tgccaccgtc ggccggaccg atcaactgta tcaggttcgc ttgccatggc tcggcaggtg
     1501 gtggtggttg cggtattgac cgctgtgatg cgtccggact gtccacattc ggagggggat
     1561 actaatgttc gattcggtga tcagcctggc caccgcgtga gcgtccgctc atcgacagat
     1621 gagtactttc gacatcttca tgacacgcgc cgaggttgca acaaagttcg tgtagtgagc
     1681 tcacctgcca gcgcaacgtg agcttcggat ccccatgatc cttcccgggt tcccggatct
     1741 caatctcgta gttaccgatc cagttttcac cgcgatcggt ctccacaggc tgctcaatcc
     1801 tggcgcagca aggaggtgcg ccaggaacga gtcaggatgg ctgacgtcct cacacacatg
     1861 ccgagccggc taacggccac catcaggtcc gcgaacggtg atcgcccaca gtcgcggctc
     1921 tgccgtttct gtctcatcgg tgttgcttgt gcgtgggtag ctgcgcaccg agcggcagct
     1981 cacgtgagac aatctgcgct cgcagctcga cagcaattca cggaccacgc cctactatgg
     2041 cttgtaccag cgacaacagt tgcatctctc acacctttag gtgacattaa cggcatccct
     2101 ggacttgagt tcgcacgacg tttggttcca ctgcggtttg gcgaccaggc ccggctcggc
     2161 gacgggtcgc cgtagggctc tgcggacggt ggacccgccg cgtagtcacg taccgcagaa
     2221 cgtgcgatac gcaccgaagc tcggcggggc cggtgcggcg tatagctcta gaccgggtcg
     2281 ttcatcgaac ggctgcgaca gcacgtcgag tagtcgccta aacggtccta ggtccccggc
     2341 actcgccgcg gccagcgctt cctccatctg atggttgcgc gggatgtaga caggattgac
     2401 ccggtccatc gcttccgcga tcgcccgtgg gtcagacgct tgggacgaca gctgtgcccg
     2461 ccaacggtcc gcccacgcgt cgaaggcgga cggttcggcg aagagcaatc gcgccggtgc
     2521 cgcatctccg cgcagtgatg acgacagtga ccggaagcat gaggtgaagt cgacgctctg
     2581 tgcatgcagc agcgctagca ggtcgtcgat caacgcgccg tcagatcgtt gggcatccgc
     2641 cacacccaac ttggcgtgca ttccgcggct ccagtactcg tcgtaacggt cggggaacga
     2701 ctgcagcacg tcggtggccg ccgcaagtgc cgtatccgcc tcgacgtgga ataggggcag
     2761 caatgtctcg gccaaccgcg ccaggttccg ctgcgcgatg cgcggctggt taccgtatgc
     2821 gtagcgtccg ccgtgatcaa tcgaactgaa cacggtggca ggatcgaagg catccatgaa
     2881 cgcgcacgga ccatagtcaa tggtctcccc ggagatcgtc atgttgtcag tgttcatgac
     2941 accgtggata aacccgacga gcatccagcc agcgaccagc gatgcctgcg cgtcgacgac
     3001 gctctcgaaa aacgcgagat acgggttctc cgcctcgacc gcattggggt ggtgacgcgc
     3061 gattacataa tcggcgaggc gtcgcacgag cgtcggtcca ttgtgcactg ctgcgtactg
     3121 gaacgtgcca acgcggacat ggctcgccgc gacacgggtg agcaccgcgc cgggcagcac
     3181 cgtatcccgc gcgacgtgat cgccggtcgc caccacagcg agcgcccggg tagtggggat
     3241 gcctagcgcg tgcatcgctt caccgatcgc atactcgcgc agcatcgggc caacgggggc
     3301 cttcccatca ccacctcgtg cgaacggcgt gcgtcccgaa cccttcaggt gcaggtcgcg
     3361 acggcgtccc tcaacgtcga acacctcgcc gagcaggagc gctcggccat cgccgaggcg
     3421 gggcacgaac ccaccgaact gatggccggc ataggcctgc gctaccggcg acgctccgtc
     3481 gggcgtggtg ttgccgacca ggacggcgac accgtcaggt gttctcagcg catcggcatc
     3541 gacacccaac tctgtggcca attcctcgtt gagcgccaac agccggggcg caggcgctgg
     3601 tgtcgcttgc cacggctcat acaggccctc cagctcacgc acataggagt tgtcgaacgc
     3661 gaacagcagc ggggcagcga cgctcataat cccgaggcta acgggccggt caccgcttgg
     3721 ggcgctcccc gggtgatcgc agggaaggcg ggcccgggcg cgggcgttgg acaggggcga
     3781 tggacgagtt ggacgccagg ctgcaccgga tcgggcagca ccacttccgc gcgaagttcc
     3841 ggctgagcgg ccgggaccgc gcggtagtcg acctgcgcgg tatcacgacc gtccgcagac
     3901 acgccgagga actggtcgga cgcaggctgg ccccggccga accgcgcaac gacggccgtc
     3961 agacacccta ccgcgggcat cccgtgttcg ttgcgcagca cgccaccgcg acgtgctgcc
     4021 gcacctgcct ggcgcgctgg cacgacatcc ccgccgggca cgcgctcgac gacgccgagc
     4081 gaggctacgt cgtggaggtg atctgccggt ggatcgccgg gcaatgcacc ccccaccacc
     4141 cgccgccgtg accggatcgg gacgccaccg cggctaacgg gtcccgggtg gcgggcaaca
     4201 agctccccgc caccctgagg gcctcctgct gccagccggt aggactggcc caccatcacc
     4261 ggccatctcg gtgtcgccga cgacaaccac cccgctgctg cgaccgttca cgacgacgtg
     4321 ccttgcagat actccgattc gaggaccagg tcccgcacga gggactggta ctcggcgtgc
     4381 gttgagtgct ccgtggtgcg ccacccggtc ccgtcccgcg cgtgcatgta ggcgcggtac
     4441 tcgggcgcgc ccgggaactt cacgttgtcg gcgacaacca ctgagccggg gtgaagccag
     4501 ccttcgtcca ggatgcgttc gaggtcaggc acgtattcct tcttcgcgtg gtcgatgaag
     4561 acgaggtcga caccaccggg gaaaaacccg tgttcctcgc ggaggcgcca cagggtggct
     4621 tcgccatccc cgagcgtccc caccacagcc gtgacccggt cggcgatccc ggcgtgctcc
     4681 cagatgcggc gggcgatcgc ggcgttggcg gcgttgaact cgattgagca gaggtgggcg
     4741 tcgggcggca tcgcccgcgc catccgcagg gcgctgtacc cgcagtacgt acccagctca
     4801 aggagcacgc gaggccggac ccgccgcacc gctgcatcaa gaatcttgcc cttctcgtca
     4861 cccacgttga tcaggaagct tttgcggtag cagaactcgt caaccacccg gatcgcgtcg
     4921 tccaggtcgc ccgctcgcgc gtgcgtggtg acgtaatcgg cgagcgcctg ctcccgaccg
     4981 tcgccgacct gccactcccg gaacaggtgc ttctgcctgg ccgccatccg caggaaggac
     5041 caccgcagga agagcacggg cctcccggcg acgtcgttag tcgcaaccgc ggccccagcg
     5101 gcagccgcgg cccaccacaa cccagcgcgg gacacgcagg gcgttcctcc tggtcgccat
     5161 ggccaacgcg gcgccggccg ccgcggcgga gacgacgaag gtgcgcgaca gctgcttcac
     5221 ggagcgacct cccggcagta gagctcagca ggaaccatct cacgggacga tgccgccggg
     5281 ctttcccgta tcgatgatcg gtactcgccg acaagctgac ctaccggttc cgctatccga
     5341 gaccgggcga catgatgggc tttgcggtac tgactcgtgg gccagtggtt caccagccag
     5401 ccatccgcaa tcggaatagt cgtgatcttc tacaaggtgc acctggcccc gtcgccggat
     5461 gtcccgcagg cggtgggacg atgaagctgt cgagtatcgc catgaccgcg ccgaccagga
     5521 cgaccgatag tgcccgccgc tgcaggtgga tcgtgggatg cgactaaaga ccgtactgac
     5581 tacgtccggc aacgcggccc ccggcggctg gtcggtccac tacgttgtga tcctgtcccg
     5641 tggttctcca ggaaatccca tggcctaggc ggtccctgtg cggcacttcg tgcgtgctat
     5701 cgcaatgctg cttgttgtcc tcgtctgtgg ctgtgaagcc accccggcgc cgccgaccgg
     5761 gagcgccgag tgggtagagc gcttgcgtca gttcgaggaa ggctatttcg cacggaatcc
     5821 gccgttcgcc gtccagaaag ggcggcacga atttgacggc cgcttgccgg actggagcgc
     5881 gaccggcatc cgggccacag tgcagtggct gcatgcgcag cgtacgcagg ctgaggcatt
     5941 cgaccagaac gcactgagcg ctgagcagcg cttcgagcgg caatacctgt tggcgcaaat
     6001 cgacaccgat ctgttctggt tggaagatgc ggagcagcca ttcaccaacc cagcctacta
     6061 tctcgacagc ggcctcgatc cgaacgtgta tctcacacgt ccttacgcct cggccgagac
     6121 tcgcctcaag gcattcatcg gctacgcgcg cgctgtgccc gctgccgctg cgcaaattcg
     6181 cgtgaacctg cgcactccgc tgccgaggag cttcatcaag tacggtgttg atagcttcgg
     6241 cgggtttcct gatttctacc gcaaagatgc gccgaaggcc tttgccgagg tgaaagaccc
     6301 tacgctgcag gccgagctgg cgagcacgat cgagccggcg gcgcgggcga tgcaagacct
     6361 cacggactgg ctcaacagcg gattaccgca ggccaccgag aacttcgcgc tcggcccagt
     6421 gaagttcgct gacatgctgc gcatgaccga gcgcgtcacc acgccggtcg ccacgctgga
     6481 gagcgtgggc cgtgctgatc tcgcgcgcaa cctcgtcgct ctcaagcagg cctgcgccga
     6541 gtatctgccg ggcgcggcga tcgaagcctg cgtagccaaa gtggaggcgg ataagccgac
     6601 tgggggggcg gtggaaggcg cgcgcgctca gctcggcatg ctgcgccagt tcgtcgtcga
     6661 tcacggcctc gtcggtattc ccggtacaga acaggccctg gttgccgaag cgcccccata
     6721 ccagcgctcg aacttcgcgt atatcgacat ccccggtccc tacgataagg gcatgccctc
     6781 ggtatattac atcgcgccac ccgatccctc atggcccaaa gccgatcagg aagcctatgt
     6841 gccgggaaag tcggtcctgc tgttcacctc agcgcacgaa gtctggcctg ggcatttcgt
     6901 acagttcctt cacgcgaacc gctcgccatt cgacttcggg cgactgttcg tcggctacgc
     6961 gtacgcggaa ggttgggcac attacaccga ggagatgatg tgggaagcgg gtctcggtaa
     7021 cggcgcgccg gaggcgcaca tcggccagct cagcgaggcg ctcctgcgaa acgtacgatt
     7081 cctgtgcgcg atcggcatgc acacgcaggg aatgagcgtg gcgagctgcg agacattgtt
     7141 ccgcgaacag gcttatcagg atccgggcag cgcgcgccag caagcagcgc gcgggagtta
     7201 cgatcctgcc tatcttaatt acacgatggg caagctgatg attcgtaagc tgcgcgcgga
     7261 ctggacggcg aaccgcggcg gacgcgccgc gtggcgagca ttccacgacg gctttctgtc
     7321 ctacggcggg ccgccaatcc cgctggtccg cgagcagatg ctgggcaccg cgggaggaga
     7381 cctgttctga atcaggccat ggcccgtgag ccctcccacg gatgctcgtg ctaacaaata
     7441 caccggagtt cagtgaggtc gctgagcgcg ctttctcacc aggagcagag cgctgttgcc
     7501 gaaaccccgc gaagcggaac ctcaatgttg gcactcgacg agagcgacga cgcatcgacc
     7561 tagcctgcct gaccttgctc tccaccgacg caatagaccc actagcacgc gtcgggcctc
     7621 gtacgagcgc atgcgatcgt gccaacgttc cgttggtgaa cccaagcgct gaacggagat
     7681 tctgatgaag cgtggcttgt ctggtgtggt ggtcctcgcc ctcggcgcag tcgcgctggc
     7741 tggctgctcc ggaggcagtg aaccagccgc ggcaacgtcg accagcgctg gattgactcc
     7801 cggcatcacc gcccgtggcc taggaacggt caccaaaatc ccggacaccg tgaccatcgt
     7861 tatcggagtc cagacccggg cgcagagcgc gaaggccggc cttgaggcca acaccgacaa
     7921 ggcgaccgcg ctgatcaacg tgctgaagag caagggagtc accccagcgg acctgcagac
     7981 cagccagctg tcggtcaacc cgacgtccga ctcggcgact gggcggatca ctggctacga
     8041 ggtgacgaac caggtcacgg ccaagctgca tgacatcggg gccgccggcg ggctcatcga
     8101 cgccgccggt gacgtagcgg gtgacgcggt gcggctacag caactcagct tttcgatcgg
     8161 ggacgacaac gcagcacggg cacaggctcg cgccaacgcg gtgcggcagg cccaggcaca
     8221 cgccaagcaa atggccgacg gggctggagt gaggctgggt cagatccgct cgatcaccga
     8281 ggtaccggcc agcccaccgg gccccttggg ccgggatgcc gcggcggctg cacccgtccc
     8341 catcgagccc ggtacccagg atctcaccgt catggtcgag gtcgtctacg ccatcgacca
     8401 gtagttctca tggggcactg ttgcggtctt ggatgccgat cgccgtgtcc cagtggagga
     8461 tcggcccgcg gacgtccaaa ggccgcttcc gtccgcggac cggcgcgatc ggcacgtgcg
     8521 ggaggctaag gggttgacgg ttgacctgaa ccaggcttga gcttctacgg tcaggctatg
     8581 gcttctactc cagagcgacc aacacctgcg ctgtcggtct tcgacgacgc ctatgcaacc
     8641 cacagcgcgc cgtgggtgat cggggaaccg cagccggcaa tagtcactct ggagcgcgag
     8701 gggtgggtcc gaggcaaggt acttgacgtc ggctgcggta ctggggagca caccatctat
     8761 ctcagcgcgc tgcgctatga cgtctacggc atggattcct cgctccgggc agtcgagcag
     8821 gcccgggcca atgccgcggc ccgcaacgtc gaggcccact tccaggtcgc cgatgcgctc
     8881 gctctgggcg atgacacgac ttacgacacc gtcatcgaca gcgcgttgtt ccacattttc
     8941 gacgcggacg accgcgcccg atacgtgcgc agcctccacc tcgcctgccg gccgggtgcg
     9001 cttgtgcacg tcctcgcgct gtcagatacc ggacccgcct tcggtccgca ggtcagcgac
     9061 gccacgatcc gcgaggcctt cagccaacga tgggtgctgg aggacttgcg ccttacagag
     9121 tatcgaggcg caatcgtccg cgacgagcac gctgtagcac ttcaccgcca gatcggcgag
     9181 ttcgttgacc tacccgcatg gctggcccga gcacgtcgga tctgacgctc gccacctgcg
     9241 acagaccact agcgactccc ccagcaatac gcgccgggaa cgggtccctc agccgagaca
     9301 ccgcgaagag cgcgggcaga gataggagaa cattatgttg ctcgatggga tcaaccacat
     9361 cgcgtggatc tccaaggacg tcgcccgctt gggtcggttc tacgccgacg tattcgacgc
     9421 cgacgtcggc ccaacgcgca cccacggcaa agacgggaaa gagacgatga ccatcatccg
     9481 catcggcccg cacaccgagt tgaacatctt cgtgatcgaa ggcaacaccg aacctgagcg
     9541 tcaaacaccc atgtgggggc gcggacgcat cgaccacgtc ggtctgcagg ccgcctcacc
     9601 agaggcgttc gccacgattc ggcaacggct gatcgagaag gcagccagcg atggcaccat
     9661 caacgacttc ggcgcggtcc acagcatctt cttccgcgac cccgatggac tcgaagggga
     9721 agtgttgatt gccaaggcgt caacctgatg caccggctcg ctttctgcta cgtacgttcg
     9781 ctccgtgttc ctgcgcgaca tcgacggcca caacgtggag gccgtgtacc acggggacta
     9841 atccgacttg gttgtccaga acacgcggcg tctcgggcac cccacggacg atccctcaac
     9901 gcatcagacc ctcacctggt gtcttgttcg cgcgggtgat gaaggtcctc gcgctcgcag
     9961 ttcatggccg agcaaccacg aggcgtcccg atcgtgaaat ccgcgggtga aaggtgcaac
    10021 agaacctggc atgactcacc ttgggataac tggatgtgcc gatcgggctg tgagccgaca
    10081 gggatgcgag catggctcgt ggtgatgggc aggagagcgt aaagtgggtc actcggcgtg
    10141 aggcgattgt gatgggcgcc tcggcgcagg cgtcgccagc ctcgtgagcc tcggaggtgc
    10201 agggatttcc ttcgcacgtg aggatgagct cgcaccggtg atcgacgggc gggtgatcgc
    10261 gttcgggatc cctggtgcat cagcggtgtc cccgatcggc acgttcctgc ccggcggccc
    10321 gatccacgat aatccgacgc tcgcggcatt cacgcagccg ggccgggtgc tcgatccgat
    10381 ccggatcatc gttggcagcc gctcgaactt tggagcgccc atagccaacc ccgaccaagc
    10441 ggcgggcgcg ctgctgtcca tcgacccagg tgaggcggag ctcgccgtac cggcgacttt
    10501 cgcgagttcg ggtgaccagg cctccgcgct gggcggggcg gtacagatgt acagcgctca
    10561 aagccccacg tggcgcaacg gcttctacaa ccctaacgcc gtgactgcgg accagacggc
    10621 ggtcagcaac ccgatcggct tgtcgatcaa taatgcgttc ggccggctgt ggcccgccaa
    10681 cgcaccgttc gggctcgacg ggccaggctc gtccacgatc gacgatccgg acggccgccc
    10741 actcaagggg gcgccgaaca cgagaactgg cggcgtctac ttcggcgacc tgaccggtcg
    10801 gcagcctgcg cagatcatcc ccggcgcgct ggctaaggcc gcggttggca cggcgttcct
    10861 gggccgctcg ccagacggaa gcggacgggc cgtcttctgc gtggtcgtgg cggacggcag
    10921 catcgtgcag gaacacaccg gcaaggcgct cgatggcctc gcgccagctg gcatcgtcgg
    10981 gccgctcagc ggtggctcgc gcgccgaaga gcgtggcgag cgcagattca cgccgcggct
    11041 gggtgcgatc atcaactaca cccccgtgct gatcctctac gtcagccaac cgttcgacga
    11101 ttcgatcaag gccatcaacc tcgagatcgg tggcccggcg ggcaatgagg tcttcgttcc
    11161 gacgacgtcg cgggccattc gctcgcaaac cctcgacata cccgttgacc tcgcgccggt
    11221 gcacatcgag tccgaggatc ccaactggtc cagcaacacg acgatggaat ctgacagcga
    11281 cttctacgtt tgcaatgccg gcaacgacac gatcgtccgc atgcgccagg acggcgccgc
    11341 ggtcgccagg tgccgggtgt gggcccgcgg ccgcccgctc ggtcacgccc gcctcaacgg
    11401 catcgcgacg tcaccagacg gtgccaagat ctgggtcacg tacgtcggcg tgctgcccgg
    11461 ttcggacgac cacgacggtg gtgtgctcga gctccccgcc ttctgatacg gaccgagggc
    11521 accgatggca tctccgcgaa cgagattagc cggtgcgcat tgagattgat cgtcgtttca
    11581 ccggttcagg cctcgagcag ccgtaaacgt cgggaactgg cacctgcctg ccgctggtcg
    11641 cggtcagccg gttcgggcgc gtaggccgtc catcagcaag tcgagcatgc gcccggcctg
    11701 gtcgcgctgt tgtggtgcac cggcggccaa aaagacgccg gtcaggctgg cgagcacgtc
    11761 gtcggcgcgg acatcgaagc gcaccgtccc ggcgaccacc gcagcgtcta gcaggctgcg
    11821 gatggcagcg gtgagtacgt ctcgcgtttc tgaggaactg atggccccgg aggcgacgac
    11881 ggcgcgcaga gcctcggcca tcccgcgctt agcggcaacg taatcagcga agcggtccat
    11941 ccaagtgcgc aaagcgacgt cggggcgtcg ggacgtgagc aggtcctcgg cgctgacccg
    12001 cagctgctca agctcgttgc gatacaccgc ctcgaccagc gcctcgcggg tggggaagtg
    12061 acggtacagg gtgccgatcc ccacgccggc gcacttagcg atcgcctcaa gcgtgacttt
    12121 ctcctcgctg gaggaaaatg cccgaagcgc cgctgccagt aaccgttgcc ggttgcgctg
    12181 cgcgtcggcc cgcacggtta tggctcccgg gccagtcatg actaagcgga gctccctccg
    12241 gatagtgtaa ggtaagagga ggttcctccg gatcgagtgt atcggtcgtc gcggggagcc
    12301 gtggaggagc gggtcatgtc cggagtcgag ggcaaggtcg tcgccatcac cggcgccagc
    12361 agcgggatag gcgaggcgac cgcgctgctg cttgccaggc gaggggcgaa ggtcgtgctt
    12421 ggggcccggc gggcggagcg tcttgaggcg ctagccaccc gtgtcaccgc agcgggaggt
    12481 gaggccgcct ttgcgcgggt cgacgtgaga cggcgtgacg acctgcgtcg cctggtcggg
    12541 ttggccggtg accagtacgg caagctcgac gtcttggtca gcaacgccgg catcggaccg
    12601 atctcgccac tggacgatct acgcgtcgag gagtgggagc agatgatcga catcaacgtg
    12661 aaaggcgtcc tgtacggcat cgcggcggcc ctgcccgtct tccgccgaca gggcttcggg
    12721 catttcgtcc acaccgcttc caccgcggcc caccgcatca cacccaacca atcggtatat
    12781 gccgccacca agacggccgt gcgcgtgatc tccgaaggcc tgcgccaaga ggccggcgac
    12841 cggctgcgag tgaccgtcat ctcgcccggc ttcacacgga ccgacttcgc agacgatgtg
    12901 accaacccgc aagtgcacgc tcagatcgcc gagtccaaag acctgatcgc catgccatcc
    12961 gacgccgtcg cccgggccat cgccttcgcg atcgaacagc ccgccgacat cgacatgggt
    13021 gagatcgtcg tccgccccac cgcacagagc tgacatccgt gacacgagcc cgataacagg
    13081 agagcgggtg acgagtggcg acggtgacgg ggtcccccgc cgtcatagcc tcatgcagta
    13141 acggctcctt gggttgctca gaagtagccg ccgtggaggg agcgtgcggg cagtgcgcgg
    13201 cggcggacca atcaagtttt ctgctggtga acctgtttcg cgactgcttt cgctgcccgg
    13261 tggtcggggt gcggcggacg tggccgtagt acctggcctg tctgagaccg ccgcaacaca
    13321 gcgattcacc gctggtgctc agtggatacc ttcactggga tacatgtcac atcgagctgc
    13381 tcgcgctcgg cattctcgac acagtgaaca tatagctgcc agtccgatga gctggcggcg
    13441 cgcggcatta cctcactcgt cgaccgtccg ttgcctcgcc accaggaagg acccgtcatg
    13501 accgacgcga cccctgccag cggagtcccc accgtcctgc tcgtccacgg cgccttcgcc
    13561 gacggctccg gctgggccgg cgtgatcgag cggttacaag ccctcgggat ccgggtgcgg
    13621 gccctggtca acccactgcg tggcctggcc agcgactccg cctacgtcgc cagcgcgatc
    13681 gagcagaccc cgggcccggt actggccgtc ggccactcct acggcggcgc ggtaatcacc
    13741 aatgccgctg cccaggccaa caacgtcgtc gggctggtct acgtcgccgc cttcgcccca
    13801 gaagaaggtg agaccctgct ggacattgag ggcagctcca gggacagcgt cctgagctcg
    13861 gccctgcggg aagcgcggta cccgatcggg cagggctcgg agacggcggt cgagctcttc
    13921 atcgacctcg catccttcca cgaagtcttc gctggcgacc tgccggcggc gcaggccgcc
    13981 gtgctgggcg cggcgcagcg gccggtggcg gccgccgcgt tcggcgagaa gtccgggccg
    14041 cccgcctgga agaacttgcc cgcctgggcg gtggt
//
LOCUS       DASYLX010000350         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3854835,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000350 DASYLX010000000
VERSION     DASYLX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3854835"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..811)
                     /locus_tag="VGP27_08575"
     CDS             complement(125..811)
                     /locus_tag="VGP27_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEV7851403.1"
                     /translation="MAETAATPPVVDQQTWRTELDELRRREKAATRELDAIAAQRRRL
                     PMVEMGDYTLVGEDGPVRLVDVFDGYSQLIVYNHMWEDGAEWQCGGCTGFTSQFTRLE
                     FLDSYDARFVIVTNGPIEEALAYRDKVGNKMAWYSSSESSFAADVDAPPGAGFGVNVF
                     LRDGDTVYRTWHTNGRGTEQLSHSFALIDLLPWGRQEEWQDSPEGWPQSPTYSRWLDS
                     PDIARVYGAT"
     gene            904..1674
                     /locus_tag="VGP27_08580"
     CDS             904..1674
                     /locus_tag="VGP27_08580"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851404.1"
                     /translation="MTRLLDDKVVIVAGLGGIGNGLARRYADEGALLVIGDLDGELSS
                     RVAAEVDPSGERVRGVALDGADESSVIGIVKLAVDTFGRLDGIHLNFTNAADAYLPGG
                     VVDLPLEAFDEVMRVNTRGFVICAKHAIPPMISSGGGSIVFTASIDAYNGAGTRVSYA
                     MSKAAELALMRHIARKYGPKGIRANAIAPGLIWHYKFDENPMPDGIVDQTRARQMIKS
                     RFGKPDDVAAMGALLLSDDGSFITAQTISIDGGVTFRP"
BASE COUNT          283 a          588 c          550 g          286 t
ORIGIN      
        1 caggtgcccg atcccgtcgg tcccgcaccc gcacaggtgc ccgatcccgt cggtccggca
       61 cccaagcagg tccccgcgcc cgaacctgca cagaaggcgt gcgatccgaa cgtggacatc
      121 tgcgtcaagt agcgccgtag acccgagcga tatccggcga gtccagccac ctcgaatacg
      181 tcggcgactg cggccagccc tccggcgagt cctgccactc ctcctggcgt ccccacggca
      241 ggaggtcgat gagggcgaac gagtggctca gctgttcggt gccacgacca ttggtgtgcc
      301 aggttcggta aaccgtgtcg ccgtcgcgca ggaacacgtt gacgccgaac ccggcgccgg
      361 gcggcgcgtc cacgtcggcg gcgaacgaac tctccgacga cgagtaccag gccatcttgt
      421 taccgacctt gtcccgatag gccagcgcct cctcgatggg cccgttggtg acgatcacga
      481 accgcgcgtc gtaactgtcc aggaactcga gccgggtgaa ttgggacgtg aagcccgtgc
      541 agccgccgca ctgccactcg gcaccgtcct cccacatgtg gttgtagacg atgagctgcg
      601 aatagccgtc gaacacgtcg acgagtcgca ccggcccgtc ctcgccgacg agtgtgtagt
      661 cacccatctc gaccatcggg agccggcggc gctgcgcggc gatggcgtcc agctctcggg
      721 tcgcggcctt ctcgcgccgt cgcagctcgt cgagctccgt ccgccaggtc tgctgatcga
      781 ccaccggggg tgtcgccgcc gtctctgcca tgaagatctc ctctcggtgc ctcgttcagc
      841 tatagacacg gccgccgccg gaaactcatc gcgccgcgcg ccgcatcggg aaggatctgt
      901 gcggtgacga gacttctcga cgacaaggtc gtcattgttg cgggccttgg cggcatcggc
      961 aacgggctgg cgcgtcggta cgccgatgag ggcgcgctgt tggtgatcgg cgatctcgac
     1021 ggcgagttgt cgtcacgggt ggccgccgag gtcgacccgt cgggcgagcg ggtgcgcggt
     1081 gttgccctcg acggcgccga cgagtcctcc gtcatcggca tcgtgaagct cgccgtcgac
     1141 accttcggcc ggctcgacgg aatccatttg aacttcacca acgccgccga tgcgtacctg
     1201 cccggcggtg tcgtcgatct gccgctcgag gcgttcgacg aagtcatgcg ggtcaacacc
     1261 cgcgggttcg tcatctgcgc caagcacgcg attccgccga tgatctccag cggcgggggg
     1321 tcgatcgtgt tcaccgcctc catcgacgcc tacaacggtg ccggcacacg ggtgtcgtac
     1381 gcgatgagca aggccgccga gctggcgctg atgcgtcaca tcgcgcgaaa gtacgggccg
     1441 aagggtatcc gcgccaacgc gattgcgcca ggactgattt ggcactacaa gttcgacgag
     1501 aatcccatgc ccgacgggat cgtcgaccag acgcgggctc gtcagatgat caaatcccgc
     1561 ttcggtaagc ccgacgacgt cgcggcgatg ggcgcgctgt tgctctccga cgacggcagc
     1621 ttcatcaccg cccaaaccat cagcatcgac ggcggcgtga cgttccgccc atgacgcagg
     1681 cgccgcccgg gaagtatcgt ccgatca
//
LOCUS       DASYLX010000351         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1441583,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000351 DASYLX010000000
VERSION     DASYLX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441583"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VGP27_08585"
     CDS             complement(<1..659)
                     /locus_tag="VGP27_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEV7851405.1"
                     /translation="MTTQESTPPDTQLGSRGSARTSPNWGRWISLIAWLGFLGLFAAV
                     ARTDVDPRVANPNIEGRPRPVGFLTGFDHWQVIPQVGALIMVVVLTIVFIRGWRKNPG
                     SPVLMMVLVTTLIVWQDPIMNWSPYAVYNPALLHWPESWPLIMMSPTVEPWIVFGYVT
                     FYFGPYFPAIWILRKIQAKRGPEAFVSRHPLISLGALVLVIGFIFDAILEISLVRTGL
                     Y"
     gene            complement(661..1779)
                     /locus_tag="VGP27_08590"
     CDS             complement(661..1779)
                     /locus_tag="VGP27_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7851406.1"
                     /translation="MSYESTAEPIKVGYLMDFTLPPGFPEKLLASFTQTFDLVFEEAV
                     EQGVMDRPVQMIYREVEGLPKGSVKAVIDAYGDLVDEGALVVFGPNITDNCVAVREAI
                     EERFKVPAISVTGTDDWLGEWTFAFPQGSMTDEPIFLTDLVVKRGLTEIGVLVEQNLI
                     GESYLQNLRSACVRKGVRIVAEAPIAQTAQDINQAVSTLYEAKAQAIVHLGFGFGIVF
                     INPALEAVGWDPPRFTTTAFQNAWVNPIMWNAFLGWIGVDQYDENNPIGQAWLDRYAK
                     KYDGSRPEYCVSVVNHDVAATMVRAFTDAHPLSPRGVKEALERVKMMPAASGAPGTRV
                     SLGKWTRRAWMGSGYLVARTLDPDGINSHLVDRFGEEA"
     gene            complement(1839..2681)
                     /locus_tag="VGP27_08595"
     CDS             complement(1839..2681)
                     /locus_tag="VGP27_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEV7851407.1"
                     /translation="MGNEPGLVHQNGAKPTAGGSRPYYRSTGNEEAVFKAAYRQGLSL
                     VLKGPTGCGKTRFVEAMAHDLGRPLITVACHDDLTTADLVGRYLLRGDETVWVDGPLT
                     HAVREGAICYLDEVVEARQDTTVVLHPLADYRRQLPIERLGITLDAAPGFGLVVSYNP
                     GYQSVLKDLKDSTRQRMVAIEFGFPSPDVEEGIVAREAGVDNATAAELVRFGQAIRRL
                     ETGGLREVASTRVLIAAGRLIAEGLTVPDAAKAAIAGPLTDDPSVGRGLNELISVYLA
                     GAPD"
     gene            complement(2759..>3838)
                     /locus_tag="VGP27_08600"
     CDS             complement(2759..>3838)
                     /locus_tag="VGP27_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7851408.1"
                     /translation="GSIGKWLKKMLSSARKSGSSGGGPPGADAPTHRTNSGNRGAFSV
                     TSAAAVTSEEIADVKQEGVKYPEWDANRKSYRPDWCTVREVDPKVKASAPQAIDGAIG
                     VRRPLARLGMGLHRRHRQSQGDDIDIDAAVEARVEVRAGSAPDEAVYLDSLRRRRDLS
                     VLLLLDVSGSAAEPGTVGHTVHQQQRAAVADLTVALHDLGDRVALYAYYSQGRTAVTM
                     MPVKRFDDHLDARVMRRLNSLEPGAYSRLGAAIRHGSSVLEDRGGTSRRLLVVLSDGL
                     AYDHGYERAYGAADARRALTEARHRGTGCVCLTVGAGTDAAVLRKVFGSAAHATIGRP
                     EQLGTVIGPMFRSALRSAEVRRRVS"
BASE COUNT          696 a         1273 c         1167 g          702 t
ORIGIN      
        1 atgtagagcc ccgtgcgcac caagctgatc tccagaatgg cgtcgaagat gaagccgatc
       61 accagcacga gggcacccaa gctgatgagc gggtgccggg agacgaacgc ttccgggccg
      121 cgcttcgcct gtatcttgcg aagaatccag atcgccggga agtacggacc gaagtagaac
      181 gtcacatatc cgaacacgat ccagggttca acggtcggcg acatcatgat caggggccag
      241 gattccggcc agtgcaacaa cgccgggttg tacaccgcgt atggcgacca gttcatgatc
      301 gggtcctgcc acacgatcaa cgtcgtgacc aggaccatca tcagcaccgg gctgccgggg
      361 ttcttccgcc agccacggat gaagacgatg gtcagcacca cgaccatgat caaggcgccg
      421 acctgcggga tgacttgcca gtgatcgaat ccggtcagga acccgaccgg gcggggccgc
      481 ccctcgatgt tcgggttggc cacgcggggg tctacgtccg tccgcgcaac ggcggcgaac
      541 aacccgagga agccgagcca cgcgatcagt gaaatccatc gcccccagtt cgggctggtg
      601 cgcgccgagc cccgtgaacc aagttgtgtg tcgggtggtg tcgattcctg tgtcgtcatg
      661 tcaggcttcc tctccgaagc gatcgacgag atgggagttg ataccgtctg gatcgagcgt
      721 ccgggctacc agatagccgg agcccatcca ggcacgtctc gtccatttac caagcgacac
      781 acgggttccc ggcgcaccgg acgcggcggg catcatcttg actcgctcta gcgcttcctt
      841 gaccccgcgt gggctcaacg gatgggcatc ggtgaacgca cgcaccatcg tggcggcgac
      901 gtcgtggttg accaccgaca cgcaatactc gggacgactg ccatcgtatt tcttcgcgta
      961 tcggtcgagc cacgcctggc cgattgggtt gttctcgtcg tactggtcga cgccgatcca
     1021 cccgaggaac gcgttccaca tgatcgggtt cacccacgcg ttctggaacg ccgtggtggt
     1081 gaatcgcggt gggtcccacc cgacggcctc gagagccggg ttgatgaaga cgatcccgaa
     1141 cccgaaccca aggtgcacga tcgcctgtgc tttggcctcg tacaacgtgc ttaccgcctg
     1201 gttgatgtcc tgcgccgttt gcgcgatcgg cgcctccgcg acgatgcgga cgcctttgcg
     1261 aacacatgcg cttcgtaggt tttggaggta gctctcgccg atcaggttct gttcgaccag
     1321 cactccgatc tcggtcagtc cccgcttgac caccagatcg gtcaggaaaa tcggttcgtc
     1381 ggtcatcgac ccctggggga aagcgaacgt ccattcgcca agccagtcgt cggtaccggt
     1441 gacgctgatc gccggcactt tgaaccgctc ttcgatggct tcgcgcactg caacgcagtt
     1501 gtcggtgatg ttcggaccga acaccaccag agcgccttcg tcgaccagat ctccgtacgc
     1561 gtcgatcacc gccttgaccg accctttggg caggccttcc acttcgcggt agatcatctg
     1621 aacaggtcgg tccatcacgc cttgttcgac cgcttcttcg aataccagat cgaaggtctg
     1681 cgtgaacgag gccaacaact tctccgggaa accaggaggc agggtgaagt ccatcaggta
     1741 accgaccttg atcggttcgg ctgtgctctc gtaggacatc cgacctccgc gatcaaacct
     1801 aatatttgtt cagacagtaa cgacggcgga gagcagcgtc aatcaggcgc cccggcgaga
     1861 tacacactga tcaactcgtt gagcccgcgg ccgacggacg gatcatctgt cagcggtcct
     1921 gcgattgcgg ctttggccgc gtcagggaca gtcaaccctt ccgcgatgag tcggcccgcg
     1981 gcgatgagga cacgtgtcga ggccacctca cgcagacctc cggtttcgag ccggcggatc
     2041 gcctgtccga accggaccag ctcggcggcg gtggcgttgt cgacgccggc ttcacgcgcg
     2101 acgattccct cctcgacgtc ggggctgggg aatccgaact cgatcgccac catccgctgc
     2161 cgggtggagt ccttgaggtc cttgaggacg ctctggtagc cggggttgta ggacacgacg
     2221 aggccgaatc ccggcgccgc atccaacgtg atgccgaggc gttcgatggg cagctgacga
     2281 cggtaatccg cgagggggtg cagcacgacc gtcgtgtcct gccgggcttc gaccacctcg
     2341 tcgaggtagc agatcgcccc ctcgcgtaca gcgtgcgtca acggaccgtc gacccacacg
     2401 gtctcatcgc ctcgcagcag ataccgaccg accaggtcgg cagtcgtcag gtcatcgtgg
     2461 cacgcaacgg tgatcagcgg ccggccgagg tcgtgcgcca tcgcctcgac gaaacgggtt
     2521 ttaccgcatc cggtcggccc tttgaggacc agcgagagac cctgccgata cgccgccttg
     2581 aagacagcct cttcattgcc tgtcgaccgg tagtacgggc gcgagccccc agccgtcggc
     2641 ttcgcgccat tctggtgtac gagtccgggc tcgttgccca tctcgcccct ttcgcctgcc
     2701 gccgctttgc gtcaccgaag cctaacccgg aacagatgtt tgtttcaatc gaacagtgtt
     2761 atgaaacccg gcgtctcact tccgcagagc gcaatgccga ccgaaacatc gggccgatca
     2821 cggtgccgag ttgctcaggc ctgccgatcg tcgcgtgggc ggcactcccg aacaccttcc
     2881 gaaggaccgc cgcgtcggta cctgccccaa ccgtgagaca tacgcatccg gttccgcggt
     2941 ggcgcgcttc cgtcagcgca cgccgagcat cggccgcccc gtaggcacgc tcgtatccgt
     3001 ggtcgtaagc cagtccgtcg gacagcacga cgagtaggcg acgggaggtt cccccgcggt
     3061 cttccaggac cgacgaacca tgccgaatag ccgcaccgag ccgcgaatac gcgcctggct
     3121 caaggctgtt cagacgcctc atcactcgtg catcgaggtg gtcgtcgaac cgcttcaccg
     3181 gcatcatcgt gaccgcagtc cgaccttgtg aatagtaggc atagagcgcc acgcggtccc
     3241 ccaggtcgtg aagggccacg gtcaagtcgg caacggctgc acgctgctgc tgatgtacgg
     3301 tgtgccccac tgttccgggc tctgcggcgg accccgaaac atcgagcagc aggagtaccg
     3361 acaggtcccg ccgccgcctc aggctgtcga ggtacaccgc ttcgtcagga gccgatcctg
     3421 ccctcacctc cacccgcgct tcgacggcgg cgtcgatgtc gatgtcgtca ccttgtgact
     3481 ggcgatggcg acgatgcaga cccatgccga gacgcgccaa cgggcgtcgc acaccgatgg
     3541 cgccgtcgat ggcctgtggc gccgacgcct tgaccttcgg atcgacttcg cggacagtgc
     3601 accagtcggg tcgatagctt ttgcgattgg cgtcccactc cggatacttg acgccttcct
     3661 gcttgacgtc ggcgatctct tccgacgtca cggccgccgc ggaggtgacc gaaaaggcgc
     3721 cccggttgcc cgaattcgtt cggtgcgtgg gcgcgtcggc ccccggaggg cccccaccac
     3781 tactgcccga tttgcgcgcc gatgacagca tcttcttcag ccacttcccg atggagcc
//
LOCUS       DASYLX010000352         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1771180,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000352 DASYLX010000000
VERSION     DASYLX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771180"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VGP27_08605"
     CDS             complement(<1..754)
                     /locus_tag="VGP27_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7851409.1"
                     /translation="MSGAAVSLLSRIILPRPGEPLDVRKLYVEESNTNARRAHAATRT
                     TLQIGAESEVSFATYFNAFPASYWRRWSTLRSVVLRAELTGAGRVDVYRSKATGARIF
                     VEGGPFHHDGTAPGSIVGTASGSIVGTASVEFEISLQPFEDGGWIWFDITTDSAVTLH
                     NAGWYAPVPAPGTANIAVGMPTFNRPDDCVDTLRALTSDPLVDEVVGAVIVPDQGTRK
                     LRDHPEFADASAPLGTRLSIHDQPNLGGSGGYS"
     gene            complement(751..1941)
                     /gene="glf"
                     /locus_tag="VGP27_08610"
     CDS             complement(751..1941)
                     /gene="glf"
                     /locus_tag="VGP27_08610"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEV7851410.1"
                     /translation="MTARYDLFVVGSGFFGLTIAERVATQLDKRVLVLDRRPHIGGNA
                     YSEPEPQTGIEVHRYGAHLFHTSNEKVWDYVRQFTDFTGYQHRVYAMHNGQAYQFPMG
                     LGLVSQFFGKYFTPDEARRLIREQAAEIETADAKNFEEKAISLVGRPLYEAFIKHYTA
                     KQWQTDPKELPASNITRLPVRYTFDNRYFNDTYEGLPVDGYTAWLKNMAADDRIEVRL
                     DTDWFDVRASLRAQSPDAPVVYTGPLDRYFDYAEGRLGWRTLDFDLEVLSTGDFQGTP
                     VMNYNDEDVPYTRIHEFRHFHPERTYPSDKTVIMREYSRFADDDDEPYYPINTESDRT
                     LLAAYRARAKAETTDARVLFGGRVGTYQYLDMHMAIASALNMYDNVLAPHLRDGASLR
                     EVTV"
     gene            2105..>2379
                     /locus_tag="VGP27_08615"
     CDS             2105..>2379
                     /locus_tag="VGP27_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851411.1"
                     /translation="MPNRRRRRLSTATSAVAALAVVSPFAYSAIADITKPAPQPREFR
                     SAAMVTDLPNELMSALSQGLSQFGVNLPALPTGLTGSPAAAMPTTLTA"
BASE COUNT          397 a          772 c          778 g          432 t
ORIGIN      
        1 ggctgtaccc gccggaaccg ccgaggtttg gctggtcatg aatcgacagc cgggtgccca
       61 gcggcgccga ggcgtccgcg aactccgggt ggtcgcgcag cttcctggtg ccttgatccg
      121 gcacgatcac cgcgccgacc acctcgtcga ccaacggatc cgacgtcaac gcgcgcagcg
      181 tgtcgacgca gtcgtcgggg cggttgaagg tgggcatacc caccgcgatg ttggccgtgc
      241 cgggggcggg caccggcgca taccagccgg cgttgtgcag ggtgacggcg ctgtcggtgg
      301 tgatgtcgaa ccagatccag ccgccgtcct cgaacggctg gagactgatc tcgaactcga
      361 cggaggcggt gccgacaatg gatcccgagg cggtgccgac aatggatccc ggggcggtgc
      421 cgtcgtgatg aaacggcccg ccctcgacga agattcgggc accggtggcc ttgctgcggt
      481 acacgtcgac ccggccggca ccggtcaact cggcgcgcag caccaccgat cgcaacgtcg
      541 accagcgccg ccagtagctc gcgggaaacg cattgaagta cgtcgcgaac gacacttccg
      601 attcggcgcc gatctgcagc gtcgttcgcg tcgccgcgtg agcgcggcgg gcgttggtgt
      661 tggactcttc gacgtatagc ttgcggacgt cgagcggttc gccggggcgc ggcaggatga
      721 tccgggacag aaggctgacc gcagcgccac tcatacggtg acctcgcgaa gcgatgcacc
      781 gtcgcgcagg tgcggagcca gcacattgtc gtacatgttc agcgcgctgg cgatcgccat
      841 gtgcatgtcg aggtactggt aggtgcccac ccggccgccg aacaacaccc tggcgtcagt
      901 ggtttcggcc ttcgccctgg cacggtatgc ggccagcagc gtgcgatcgc tctcggtgtt
      961 gatcgggtag tagggctcgt cgtcgtcatc ggcgaagcgc gagtactccc gcatgatcac
     1021 ggttttgtcg gacgggtagg tgcgttcggg atggaagtgc cggaattcgt ggatacgcgt
     1081 atagggcacg tcttcgtcgt tgtagttcat caccggggtg ccctggaaat caccggttga
     1141 cagcacctcg agatcgaaat ccagtgtccg ccagcccaat cggccctcgg cgtagtcgaa
     1201 ataccggtcc agggggccgg tgtagaccac cggcgcgtcc gggctctgcg cgcgtagcga
     1261 ggcccgcacg tcgaaccagt cggtgtccag ccgcacctcg atgcgatcgt cggcggccat
     1321 gttcttcagc cacgcggtgt agccgtcgac cggcaggccc tcgtaggtgt cgttgaagta
     1381 gcggttgtcg aacgtgtagc gcaccggcag ccgggtgatg ttgctcgcag gcagttcctt
     1441 cgggtcggtc tgccactgct tggcggtgta gtgcttgatg aacgcctcgt acagcgggcg
     1501 cccgaccagg ctgatcgcct tctcctcgaa gttcttcgcg tcggcggtct cgatctcggc
     1561 ggcctgctcc cggatcagcc tgcgggcctc gtccggcgtg aagtacttgc cgaagaactg
     1621 cgacaccagg cccaggccca tcgggaactg gtaggcctgc ccgttgtgca tcgcgtacac
     1681 ccggtgctgg tatccggtga agtcggtgaa ctgccggacg tagtcccaca ccttctcatt
     1741 ggaggtgtgg aacaggtgcg cgccgtagcg gtgcacctcg atgcccgtct gcggctcggg
     1801 ctcggagtac gcgttgccgc cgatatgcgg gcgtcggtcc agtaccagga ctcgcttgtc
     1861 cagctgggtc gccacgcgct cggcgatcgt cagaccgaag aatccggaac cgacgacaaa
     1921 gaggtcataa cgagcggtca tcggcagcca gggtatccga ccaggcttgc gccgcccgaa
     1981 tccaatacgg gctcaatgtc gcgattcgct cacgattcca tatcgtcact ctattcacac
     2041 cagtcacagc cgtaccatcg atcacgtacc gctctatacg tagtccacat attgaggagt
     2101 tcccgtgcct aaccgacgtc gacgcaggct ctcgacagcc acgagcgcag tcgccgccct
     2161 ggccgtcgtg agtccttttg catattccgc catcgcagac atcaccaaac ccgcgccgca
     2221 acctcgtgag ttccgatcgg ccgcgatggt caccgacctg cccaacgagc tgatgtccgc
     2281 gctttcgcaa gggttgtcgc agttcggcgt caacctaccc gccctgccga ccggcctgac
     2341 cggcagcccg gcggcggcga tgccgacgac actgaccgc
//
LOCUS       DASYLX010000353         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1990899,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000353 DASYLX010000000
VERSION     DASYLX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990899"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VGP27_08620"
     CDS             complement(<1..1112)
                     /locus_tag="VGP27_08620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV7851412.1"
                     /translation="MATFTTASQRATALAAALFLIASPALANAEPNAAPDACPYQTST
                     PPAVDSSEVPTAGDPPQPLAVPGDPVGGDALGGCGVITAPGTPPVPNDISAEAWLVAD
                     LDTGDVIAAKDPHGRHRPASIIKVLVATEALRELPIHKVVAGTAEDAGAEGTRVGIGE
                     GGHYSIRDILHGLLMHSGNDAAHALAMQMGGMDATLTKLNDLASKLGGRDTRVATPSG
                     LDGPGMSTSAYDIGLFYRYAWQNPTFAEIVATRSFDFPGRGDAGYPVENDNQLLYNYP
                     GAMGGKTGYTDDAGQTFVGAADHGGRRLVAVLMRGTRQPIAPWEQAAHLLDYGFATAP
                     GTKVGTLVEPDPSLVATPEKAADDSQLKAVAALPAA"
     gene            1425..>1873
                     /locus_tag="VGP27_08625"
     CDS             1425..>1873
                     /locus_tag="VGP27_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851413.1"
                     /translation="MSDALMDKQAVSDVLKDKNIIGIGSVKPMAEPEVLRAIEEWLGG
                     WYIVDHSACGNANLPATHGDTWGFINGRAAWQKKNFVYGNADVWTDDFKSGSDHYAAS
                     SLPAGFDGADSVVVGYIATHGVTSGTTFTMSAGGTGHGGCTVRSTQMS"
BASE COUNT          304 a          584 c          651 g          334 t
ORIGIN      
        1 tcggcggccg gcagcgcggc gacggccttg agttgactgt cgtcggcggc cttctccggc
       61 gtcgcgacaa gcgagggatc cggttcgacc agcgtgccca ctttcgtgcc cggcgcggtg
      121 gcaaagccgt agtccagcaa gtgcgcggcc tgctcccacg gcgcgatggg ctgccgggtg
      181 ccacgcatca gcaccgcgac cagcctgcgg ccgccgtggt cggctgcgcc gacgaacgtc
      241 tggccggcat cgtcggtata acccgtcttg ccgcccatcg cgcccgggta gttgtaaagc
      301 agttggttgt cgttctcgac cgggtagccg gcgtcgccgc ggccggggaa gtcgaagctt
      361 cgggtcgcga cgatctcggc gaacgtcggg ttctgccacg cgtagcgata gaacaggccg
      421 atgtcgtagg ccgacgtgct catgccgggg ccgtcaagac ccgatggcgt cgcgacccgg
      481 gtgtcgcggc cgccgagctt gctcgccagg tcgttgagtt tggtcagtgt ggcgtccatg
      541 ccgcccattt gcatggccaa cgcgtgggcg gcgtcgttac cggagtgcat gagcagcccg
      601 tgcaggatgt cgcggatcga gtagtggccg ccctcgccga taccgaccct ggtgccttca
      661 gcgcccgcgt cttcggcggt gcccgccacc accttgtgga tcggtagttc ccgcagtgcc
      721 tccgtggcca ccagcacctt gatgatgctg gccggccgat gcctgccgtg cggatccttg
      781 gcggcgatga cgtcgccggt gtcgaggtcg gccaccagcc aggcttctgc cgagatgtca
      841 ttgggcacgg gcggtgtgcc cggtgcggtg atcacgccgc agccgccgag cgcgtcacca
      901 ccgaccggat cgccgggtac cgcaagcggt tggggcgggt cgccggccgt cggcacctcc
      961 gacgagtcga ccgcaggcgg tgtgctggtc tgatacgggc aggcatcggg tgcggcgttc
     1021 ggttcggcgt tcgccagcgc cgggctcgcg atcaagaaca gtgccgctgc cagggcggta
     1081 gcgcgttggg acgcggtcgt gaaggtcgcc atcgtgaccg cagattaggc gattcggcgc
     1141 gcgaatcagg ggagccacgc tgtgactgtt gtggttggag tttccaaagt gactccctcg
     1201 ggcgcgccca aactcacatt tgggcggaaa agtgcgagac gagcgcgcca gaacgtcgat
     1261 ctcggtgccc ggcgcgtggt ggctacaggt cggcgctggc cagatcggtg cgtccccgca
     1321 gagtcatcgg caagtggcgt agccggttac tcgtgtcagg ccacgcacgc cgttcttaca
     1381 gtcgcctcga cgtcgtatta caggaatcgt cacaggaggc gaccatgtcg gatgctctga
     1441 tggataagca agccgtgtcg gatgttctga aagacaagaa catcatcggc atcggatcgg
     1501 tcaaacctat ggccgaaccc gaagtgctgc gtgcgataga agaatggctc ggcggctggt
     1561 acatcgtcga tcacagtgcc tgtggaaacg ccaatctgcc tgcgacgcac ggggatacat
     1621 ggggattcat caacggccga gctgcctggc agaagaagaa cttcgtatac gggaatgccg
     1681 acgtctggac cgacgacttc aagagcggct ctgaccacta cgcggcaagc agccttcctg
     1741 caggtttcga cggcgcggat tccgttgtgg tcggctacat cgctacccac ggcgtcacgt
     1801 cgggcaccac cttcacgatg agcgcgggtg ggaccggcca cggcggatgc actgtcaggt
     1861 caacccaaat gtc
//
LOCUS       DASYLX010000354         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3196519,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000354 DASYLX010000000
VERSION     DASYLX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196519"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VGP27_08630"
     CDS             complement(<1..837)
                     /locus_tag="VGP27_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851414.1"
                     /translation="MTIEQSAPPVWAGHTRGSTAYGRLLVALFCAGVATFAQLYSPQA
                     VLPLISADLHIGAAAAALTISASTVGLAIGVIPWSTLADRIGRVRAISLSVTVATAVG
                     LLVPFAPTYQLLLAGRFVEGLVIGGVPAIAIAYLTEEIDPAHAARAAGTFVAGTTIGG
                     LLGRLVSSPVAEIAGWRIGVFTVAVLCGLAAVSFVKLAPQPQGFVPSNQSGANPEGSL
                     ANRLLVNLRSPRQLALFAQGFLLMGGFVALYNFLGFRLTAAPFFLPQFVVSLVFAAYL
                     AGT"
     gene            complement(894..1775)
                     /locus_tag="VGP27_08635"
     CDS             complement(894..1775)
                     /locus_tag="VGP27_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7851415.1"
                     /translation="MNLEELRWFVVLAETEHVTDAAAALTVSQPTLSRALARLEREAG
                     TPLFDRVNRRLRLNPYGQIMLEHARRSIAEMRSATDRIAALRDPDTGRVRLAFLHSLA
                     NWYVPEQLRRFRESAPAIRFDLLQGPAHEITQRVLDGQADIAITAPRPDAPGLAWRRL
                     YVDQLCLAVAHGHRLANRARVRLSDAAGETFIALGEQAGLRQLTDQLLADDDVKPDIV
                     FEATEIPTVEGLVAAGFGVAVVPVPRDGGKSRIVHVPLTNAGAKRDVGLAWDRNRQLP
                     QPAKRFADFLSSDAIKT"
BASE COUNT          383 a          658 c          689 g          309 t
ORIGIN      
        1 ggtcccggcg aggtatgccg cgaacaccag gctgacaacg aactgcggca ggaaaaaggg
       61 cgcggcggtc aaccggaatc ccaggaagtt gtagagcgcg acgaagccgc ccatgagcag
      121 gaatccctgc gcaaagaggg ccagctggcg cggtgagcgc aggttgacga gcaggcggtt
      181 ggcaaggctc ccttcgggat tggcgcccga ctggttcgac ggaacgaagc cctgcggttg
      241 gggggcgagt ttgacaaagc tcacggcggc gaggccgcac agcaccgcga cggtgaacac
      301 gccgatgcgc catcccgcga tctccgcgac gggactggac accagccgac ccaacaggcc
      361 gccgatggtg gtgcccgcga cgaatgtccc cgccgccctt gccgcgtgtg cggggtcgat
      421 ctcctcggtg aggtaggcga tcgcgatcgc cggtacgccg ccgatcacca gcccttcgac
      481 gaaccgcccc gcgagcaaca gctgataggt gggcgcgaag ggcacgagca ggccgaccgc
      541 cgttgcgacg gtgaccgaca gcgatatcgc cctgacccga ccgatgcggt ccgccagcgt
      601 ggaccagggg atcacaccga tggcgaggcc cacggtggac gccgagatcg tcagcgccgc
      661 ggcggccgcg ccgatgtgaa ggtcggcgga gatcagcggg agaacggcct gcggcgagta
      721 gagctgggca aaggtcgcca cccccgcgca gaagagggcg acgagcagcc ggccgtacgc
      781 ggtcgacccg cgggtatgcc cagcccacac cggtggggcc gactgttcga tggtcactac
      841 aacgaagcta gatcgcgctc cttaatgtgt ccaatgcatc gatatcaaca acttcatgtc
      901 tttatcgcat cactgctgag aaagtccgcg aaccgcttcg ccggttgcgg caattgccga
      961 ttccggtccc atgccaggcc cacatcccgc ttggcacccg cgttcgtcag cggcacgtgg
     1021 acgatccgcg acttgccgcc atcgcgcgga accggcacga cggcgacccc gaacccggcc
     1081 gcgaccagtc cttccaccgt ggggatctcg gtggcttcga agacgatgtc gggcttgacg
     1141 tcatcgtctg cgaggagttg atcggtgagt tgtcgcagac ccgcctgctc accgagtgcg
     1201 atgaaggtct cgcccgcggc gtccgacagc cttaccctgg cgcggttcgc gagccggtgt
     1261 ccgtgcgcaa cggcgagaca cagttgatcg acatacagtc ggcgccaggc caggccgggc
     1321 gcatcgggac gtggcgcggt gatcgcgatg tcggcctgtc cgtcgagcac ccgctgggtg
     1381 atctcgtgcg caggtccctg aagcagatcg aaacggatcg ctggggccga ctcgcgaaag
     1441 cgccgtagtt gttcgggcac ataccaattc gccagtgaat gcaggaaggc gagccgaacc
     1501 cggccggtgt ccggatcgcg aagggcggcg atacggtcgg tggcagacct catctcggcg
     1561 atgctgcgcc tggcgtgctc gagcatgatc tggccatacg ggttcaatcg gagcctgcgg
     1621 ttgacccggt cgaacagcgg tgtgcccgcc tcacgctcaa gtcgcgccag ggcccgggac
     1681 agcgtcggct ggctgaccgt caacgcggca gcggcgtcgg tgacgtgttc cgtctcggcg
     1741 agcaccacga accaccgcag ttcctccagg ttcaccgttc gaccataacg aaaatgcatg
     1801 atgcggagag atcacttgtg attcatgcat gaccggtgcc gattcctgcg tcgctacact
     1861 gcctgcattc aacgtcgggc gtgaatcagc gacggtgtcc gaaatcggag ggcccagtga
     1921 ccgagaacga cgaaaatctc gagcgcgcga tcctgcgcgc gctcgacggc ctgctcagct
     1981 cactgcagcc ggaagccatg ggcgacgacc gctttcgcgt tcacagtgaa gcgaaccgg
//
LOCUS       DASYLX010000355         7538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3307501,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000355 DASYLX010000000
VERSION     DASYLX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307501"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="VGP27_08640"
     CDS             <1..2250
                     /locus_tag="VGP27_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7851416.1"
                     /translation="GMFVDRRQVLSVDVRDGQIINVSNPDGPQLTFELGRPTGAVGRP
                     PSAVPLVGPPRNGQPLSSRPPEQVGATDSLPTSAIPTSIRLSARRGGDGSNLALSIIR
                     ILRGPSAAAPGAVTVGRATDNDIVLPDVLASRHHATLLPTPEGVKIVDARSINGTFVN
                     GTRVDQALLAENDLVTIGNADLLFRGGTLVRRNETRAATRAGGLQVRAISLTIEHGQE
                     LLNNISFGAEPGTLTAVIGPSGAGKSTLAKVIAGTARPSRGKVSFEGHDIHGQYASLR
                     SRIGMVPQDDVVHRQLTVKQALGYAAELRLPPDTTKHDRREVLGQVLEELELKAHART
                     RVDRLSGGQRKRASVAMELLTGPSLLILDEPTSGLDPALDRQVMTMLRQLADAGRTVV
                     VVTHSLTFLDMCDQVLLLAPGGKTAFCGPPSGIGRALGTTNWADIFTNIGLDPDAANR
                     RFLQQTTPPPAPPDTDKPTDLGKPVHTSLRRQFSTIGRRQLRLIVSDRTYFAFLALLP
                     IVLGVLSLAVSGNVGFGIPNPMGDAPDEPGQILVLLTMGAIFMGTALAIRDLIGERTI
                     FRREQAVGLSATAYLLAKICVYTAAAVIQSAILVTIVVHGKGGPTRGALMLGNPTVEL
                     FVTVAGTCITGAILGLALSAVARSNDQIMPMLVVLVISQLVFCGGMIPVAHRLPLDQL
                     SWFTPARWGFAASASTADLITLVPTPQTKDSYWQHTPTTWIFDMGMLALLGLGYALLA
                     RWKIRLDCE"
     gene            complement(2774..3433)
                     /locus_tag="VGP27_08645"
     CDS             complement(2774..3433)
                     /locus_tag="VGP27_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851417.1"
                     /translation="MDLMWGVIALTVATFTVLLAVRRFLMVGSRLVFSRYVAVGGLVS
                     FSTDRDGSADPHDSIDHHCGGVYHVRFEVGGPGVFHNVSVQLFGVPESGQGAEVKRPA
                     VRHTMAAGDGPIEWTFRLPGEAAERAWVMVTWVRPYLQGVESEALAQWLLTGRLYEWR
                     WYSETTRYVRTVIRFVARRWSMPAGESVRELPLYGRWRRTSSNTSADLLGPATNPPPM
                     V"
     gene            complement(3736..4842)
                     /locus_tag="VGP27_08650"
     CDS             complement(3736..4842)
                     /locus_tag="VGP27_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HEV7851418.1"
                     /translation="MQPRRPGESGRPTPSAADLRRWRQHLADERAEAAVYRDLAGRRT
                     GEERDILLALADAEARHEQHWLTMLGDDVGKPRRGDVRSRILGFLARHFGSVFVLALA
                     QRAEARSTYDADVDATATMAADERIHGEVVRALAARGRSRLSGSFRAAVFGANDGLVS
                     NLALVLGVSASGVSNHTVLVTGAAGLLAGALSMGAGEYVSVRSQRELLQASTPDPHAG
                     AALRALDVDANELALVYRARGMSEAEAVKHANEILGNQVSATIGQFSFTATDPAEHEA
                     IGTGLRAAVSSFSFFASGALIPVLPYLFGLQGAVALVIAAVLVGIALICTGVIVGLLS
                     GGPPLRRALRQLLIGYGAAAVTYLLGLLLGTGLG"
     gene            5350..5850
                     /locus_tag="VGP27_08655"
     CDS             5350..5850
                     /locus_tag="VGP27_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851419.1"
                     /translation="MNPGLDEVFPPTPQVWAELAQHEMAGDHQFGKAVGFLEEGGTNQ
                     NLLAERLNIKVDYANWLLHHARKMKSGIIRVVVSEKNNGRETASIQAHHYRYVLDDDL
                     TPETRHYVETVIAKFRTINPDIPMKAAKAQGAQDPYDRELRSEPALCTNPDCEWAWTR
                     HGGQCS"
     gene            5847..6065
                     /locus_tag="VGP27_08660"
     CDS             5847..6065
                     /locus_tag="VGP27_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851420.1"
                     /translation="MTLDPSAEMRKGHAVIDTDSVRSFTDEQVLQEFGKAMLAHSYGY
                     YDFTDRMEVLRAEALRRMGESKAEGKPR"
     gene            complement(6137..6352)
                     /locus_tag="VGP27_08665"
     CDS             complement(6137..6352)
                     /locus_tag="VGP27_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851421.1"
                     /translation="MASLRNGTRKDGSTYVQVLYRLDGGKQSSTSFEDMASATKFKKL
                     VEKFGPTEARETLGTATVYRYLADEAA"
     gene            complement(6439..6807)
                     /gene="ssrA"
                     /locus_tag="VGP27_08670"
     tmRNA           complement(6439..6807)
                     /gene="ssrA"
                     /locus_tag="VGP27_08670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6937..7506
                     /locus_tag="VGP27_08675"
     CDS             6937..7506
                     /locus_tag="VGP27_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851422.1"
                     /translation="MTDHTIKLVVDEGVPARIADTLMKALPDWQVCFANEPIPLDEDG
                     GIPMVDLARRYRSDQGSDVVVMITELPLRVRTTPLVADYNMKAQVALFSLPAFGVMRV
                     RQRLCGVAVPLIAHLSGRDATFPGRMGALRIPVRRISADAPGGNQHLALTGIRVACKC
                     WPGWYSPIGRGAWSRRCRALPLQPPPLQA"
BASE COUNT         1448 a         2457 c         2335 g         1298 t
ORIGIN      
        1 ggaatgttcg ttgaccggcg tcaggtgctg tcggtagacg tccgcgacgg gcagatcatc
       61 aacgtcagta atccggacgg gccgcagctg actttcgagc tcggccggcc caccggcgcc
      121 gtcggcaggc cgccgagcgc ggtccctcta gtcggaccgc cgcggaacgg gcagcctttg
      181 tcgagccggc caccagagca ggttggcgca acggactcgc tacccacctc tgctattccc
      241 acctcgatca ggctctcagc gaggcgcggc ggcgacgggt cgaacctggc gctcagcatt
      301 atccggatcc ttcgcgggcc gtcggccgcg gcgccgggcg ccgtcacggt cgggcgcgcc
      361 accgacaacg acatcgtgct tcccgatgtg ctggcgtcac gtcaccacgc cacgttgttg
      421 ccaacccccg agggcgtcaa gatcgtagac gcgcgcagca tcaacgggac attcgtcaac
      481 ggcacccgcg tcgatcaggc gctgttggcc gagaacgatt tggtgactat tggcaacgcc
      541 gatctgcttt ttcggggtgg caccctggtt cgtcgcaacg aaacccgagc cgccacccgc
      601 gccggcggtc tgcaggtgcg cgcgataagc ctgaccattg agcacgggca ggaactgctg
      661 aacaacatct cgttcggcgc agagccaggc acactgaccg cggtgatcgg cccgtcgggt
      721 gccggcaagt cgacgttggc caaagtcatc gccgggaccg cccgccctag ccgcgggaaa
      781 gtctcgttcg agggacacga catccacggc caatacgcct cgttgcgcag tcgtatcggg
      841 atggttcccc aggacgacgt ggtgcataga cagctcactg tcaagcaggc gctcggctac
      901 gccgcggagt tacggttgcc gccggatacc accaagcacg atcggcgcga agtgttgggc
      961 caggtgctcg aggaactgga actcaaagcc cacgcccgga cccgagtcga tcggctctcc
     1021 ggcggtcagc gcaaacgcgc gtcggtggcg atggaactgt tgaccggccc ctcccttttg
     1081 atactcgacg aacccacctc tgggctggac cccgcactgg accgccaagt gatgacgatg
     1141 ctgcgccaac tcgccgacgc cggccgcacc gtcgttgtgg tgacacactc gttgaccttc
     1201 ctcgacatgt gcgatcaggt gctgctgctg gcccccggag ggaagaccgc gttttgcggg
     1261 ccgcccagcg ggataggccg ggcgttgggc acgacgaact gggccgacat cttcaccaac
     1321 attgggctcg atcccgacgc ggccaatcgg cgattcctgc agcagacgac cccgccgccc
     1381 gcgccgccgg acaccgacaa gccgaccgat ctgggcaagc cggtgcacac cagcttgcgg
     1441 cggcaattct caactattgg acgccgccaa ctgcgcctca tcgtgtccga ccgcacctac
     1501 ttcgcgttcc tggcactgct cccgattgtc ctgggtgtgc tatcgctagc cgtgtcggga
     1561 aacgtgggct tcggtatccc caacccgatg ggcgatgccc ctgacgagcc aggccaaatc
     1621 ctggtcttgc tgaccatggg ggcgatcttc atgggaaccg ctctcgcgat tcgagacctc
     1681 atcggcgagc gcacgatctt ccgccgtgag caggcagtgg ggctgtcggc gacggcgtat
     1741 ctgctggcca agatctgcgt atacaccgcg gcagcggtca tccagtcggc gatcctggtg
     1801 acgatcgttg tccacggcaa aggcggtccc acccgaggtg ctctgatgct cggcaaccca
     1861 accgtcgagc ttttcgtaac cgttgcggga acctgcatca caggggcgat actcggcctg
     1921 gcgctgtctg ccgtggcccg ctccaacgac cagatcatgc cgatgctggt ggtgctggtc
     1981 atctcccagc tggtgttctg cggcggcatg atccctgtgg cgcaccgtct gccgctcgat
     2041 cagctgtcct ggttcacacc ggcccgatgg ggctttgccg cgtcggcctc gacggcggac
     2101 ttgatcacgc tggttccgac accgcagacc aaagattcgt actggcagca cacgccgacg
     2161 acgtggatct tcgacatggg catgctcgcc ctgctgggcc tcggttacgc cctgctcgcc
     2221 cgctggaaga ttcggctcga ctgtgaatag gcgcaatgtc cgccgatcgt cggtttggtc
     2281 acgacatctg gtgcgtactt ccgggctgac gacgacggtg gtgcgatcgg acgaccgaat
     2341 tgtgcggcgc gctgaaggcc aaggccgaca agcatattgg cacaccggtt ggacgagccc
     2401 tgcaactcgg agaagggtag cggcacgtgt ccccatccgg ttgctcgtgg atgagcgcaa
     2461 tcccgctcga aaacaccgct caaaatcaca acgctttttc tcaattgcac cgtgcctgct
     2521 cacactcgtg atccaaaaaa gggccgcatc tctacgacct tctggtggag ctggcgggaa
     2581 tcgaatccgc aagtagcgcg gttgacctgt gcacacgaga gacgagtcaa cacggtccga
     2641 catggttgtt tgcgagtgtt gacgggcgtt cagttgacct acgtcaacac aaaggttggc
     2701 tgacgacacg acgtcaggcg gtcgagcaac tgccatcgcc tgcaaaacag ccgtccaggc
     2761 cgtggaagac ggcttagacc atcggcggcg ggttggtagc ggggcccaac agatcggcgc
     2821 tggtgttcga cgaggtgcgc cgccaccgcc cgtacagcgg caactcgcgc accgactcgc
     2881 cggccggcat cgaccaccgt cgggcaacga accgaatcac ggtccgcacg taacgggtcg
     2941 tctcggagta ccaccgccat tcgtagaggc gaccggtcag caaccattgc gcaagcgcct
     3001 cggattcgac gccctgcaga tacggccgaa cccacgtcac catcacccac gcccgctctg
     3061 ccgcttcgcc gggaagccgg aaggtccact cgatcggacc gtcaccggcg gccatggtgt
     3121 gccgcaccgc gggccgtttc acctcggcac cctggcccga ttccggcaca ccgaacagtt
     3181 gcaccgaaac gttgtgaaag actccgggcc cgcccacctc gaaccgcacg tggtaaacgc
     3241 caccgcagtg atgatcgatc gagtcgtggg ggtcggcgga gccgtcgcgg tccgtggaga
     3301 acgaaaccaa cccgccgact gctacatagc gggagaacac taaccggctt cccaccatga
     3361 ggaaccggcg caccgccagc agcaccgtga acgtcgccac cgtcagagcg atgacacccc
     3421 acatcagatc cacccgcaca gagtgtcaga gctgcacaag aaccgggtga caacactcca
     3481 acacaggaaa ggccccactc ctgagggcct gacctgctat cgaattgtgg agctgccggg
     3541 gatcgaaccc gttactgaac ggcagtgacc tgcacaaaca ctgattctga caacgcgaaa
     3601 tcaactgaaa tgacccgcaa ctacctgcgg atacgcgaaa ggtgttgacg gcgtcaacac
     3661 cttcgccggc ctatgcagtc tgttgctggg gtgctaaggg cgagaatggc gcggctgagc
     3721 gtacggcgtg tccgcctaac cgagtcccgt gcccagcagt agacccagta gatatgtgac
     3781 tgcggcggcg ccgtatccga tgagaagctg gcgcagcgcg cgtcgcaggg gcggtccgcc
     3841 agacagcagg cccacgatca caccggtgca gatcagcgcg atgccgacga gtaccgccgc
     3901 aatgacgagc gcgacggccc cttgcagccc gaacaggtag gggagcaccg gtatgagagc
     3961 cccagaagcg aagaaggaga agctcgaaac ggcggcgcgc aagccggtgc cgatcgcctc
     4021 gtgctcggcg ggatcggttg cggtgaagct gaattgccct atcgtcgccg acacttggtt
     4081 gccgagaatc tcgttggcat gcttcacagc ttccgcctca ctcataccgc gtgcccggta
     4141 gaccaacgcc agctcgttgg cgtcgacgtc cagcgcgcgc agggcggcgc ccgcatgggg
     4201 atccggagtc gacgcctgca gcagctcccg ttgcgagcgc accgagacat actcgccggc
     4261 gcccatcgac aacgcgccgg cgagcaggcc ggctgcaccg gtgacgagca cagtgtggtt
     4321 cgacactccg ctggcgctga cccccaacac cagcgcaagg ttgctgacca gcccgtcatt
     4381 ggcaccgaac acggcggcgc gaaaactccc ggaaaggcga ctgcggcccc gtgcggccag
     4441 cgcccgaacc acctcgccat ggatccgctc gtcagcggcc atggtggcag tggcatcgac
     4501 atcggcgtcg taggtggacc tcgcctcagc gcgctgggcg agggcgagca caaagaccga
     4561 tccgaaatgc cgcgccagaa accccaaaat gcgcgagcgt acatctccgc gccggggctt
     4621 tcccacatcg tcaccgagca tggtcagcca gtgctgctcg tgacgagcct ccgcgtcggc
     4681 cagcgccagc aggatgtcgc gctcctcgcc ggtgcgtcga ccggccaggt cccggtacac
     4741 ggccgcttca gcccgctcgt cggccaagtg ctgacgccaa cgccgaagat cagcggcgga
     4801 cggtgtcggc cggccggact cgccgggtcg tcggggttgc accaacccat gatgcggcag
     4861 gacgcggccc tcgcatcgcg cgacttggtg gatgtagggg gtctcccaca tcgccggatg
     4921 aggtggtcgg agagcctccg cacgcggaag tgctagcact tgcacttttg gcagttgcca
     4981 actcagccgc caaagggccg cacatctgcg gccctttcga acctcgtcat caagtctcgg
     5041 ccatccggga atagcgggcg accgcactgc caagtggtca ggcgagctgg cggcgcatcg
     5101 cctcggcggt cagttcggaa cggatgcgag tgccaacgat cgattctctg gacccgcttt
     5161 tagggcggac ctatccgaac cgcgcctggc atccactcga cggcaggatc atcgagctgg
     5221 gcatgcacgt gaccgttgac cccgctctcc gctacgagcg cgtgatcggc atatcagccg
     5281 ccccggcgtg accgaatgtc accattcgtg cgatcacgtt gctcaatggg gatcagagaa
     5341 ggagaacgaa tgaatccggg actcgatgaa gtcttcccgc cgacgccgca agtgtgggct
     5401 gagttagccc agcacgagat ggcaggtgat caccagttcg gtaaggctgt tgggtttttg
     5461 gaagaaggtg gcaccaacca gaacctcctc gcggagcgat tgaacatcaa agttgactat
     5521 gccaactggc tgctgcacca cgcccggaag atgaaaagcg ggattatccg cgtcgtggtt
     5581 agtgagaaga acaacggtcg ggagacggcg agtatccagg ctcaccacta ccggtacgtg
     5641 ttggacgacg acctcactcc tgaaacccgt cactacgtgg aaacggtgat agcgaagttc
     5701 cgaaccatca accctgacat cccgatgaag gcggcgaaag ctcaaggtgc tcaggatccc
     5761 tacgaccgcg aactgagatc cgaaccggcg ctttgcacca atcctgattg tgaatgggcc
     5821 tggactcgtc acggtggtca atgctcatga cgctggaccc cagcgccgag atgcggaaag
     5881 ggcacgcggt catagatacc gactcggtgc gatcgttcac cgacgagcag gtgctccaag
     5941 agttcgggaa agcgatgctc gcccactcct atggctacta cgacttcacc gaccgcatgg
     6001 aggtcctgcg ggcggaggca ctgcggcgaa tgggcgagag caaagctgaa ggaaagccta
     6061 ggtaggcccg tggttagtcg tgggttgcca cctcgacact cgggcgactt ccaggaggtg
     6121 ttctacacaa gcggcgttag gcagcctcgt ctgccaagta gcggtagacg gtggccgtgc
     6181 caagggtctc ccgcgcttct gttggtccaa acttctcgac caacttcttg aacttggtgg
     6241 ccgacgccat gtcctcaaag gacgtggagc tctgcttgcc cccgtccaac cggtagagca
     6301 cctgcacata ggtgctcccg tccttgcgag tgccgtttcg gagggacgcc attccgccag
     6361 cgaaaccgaa acatgttgat ggagtgttga tggaaaaaga aaacaggcca gggattgtgt
     6421 ccctgacctg catcttcttg gtggagctgc cgggaatcga acccgggtcc tacggcactt
     6481 cctcgaggct tctccgtgcg cagtccgcta agcctctact cggatctcct gatcacgcga
     6541 acaagtcagg atgacgatcc cagtcgctgt ttggtgtccc cgtacatccc gcgaccggac
     6601 gtatgggttt gtccctctag ctgatgccag ggtccgggcc gagggcgctc ccggtctgac
     6661 agacacgcag tcgcttaggc agcgagtgcg aagtcgcgct gattggaatc ggcgcttaat
     6721 tggttgcaat gacgcttacg gtggtctctt gcctgcaccg gcacgcttcc cttgattcga
     6781 tgcgcgaagt cgaaaccgtt cagcccctcg cacacctgcc ggatttcggc aggacaatca
     6841 atggtacgcc tgttacaaca gagatcttca agtgtttcat cccgtgtccg cgggcaccgg
     6901 ccgcatcacg ccgatcccgg cgggtacccg gtaccaatga ccgaccacac catcaaactc
     6961 gtggtcgatg agggggtccc cgccaggatc gcagacaccc tgatgaaggc gctaccggac
     7021 tggcaggtgt gcttcgcgaa cgagccgata ccgctggatg aggacggcgg catcccgatg
     7081 gtcgacctcg cccggcgtta ccggtcggat cagggttccg acgtggtcgt catgatcacc
     7141 gagctacccc tgcgggtgcg caccacaccc ctcgtcgccg actacaacat gaaagcccaa
     7201 gtagcactgt tttcactgcc cgcattcggc gtcatgcgag tgcggcaacg attgtgtggt
     7261 gtggctgtac cgctgatcgc acacctttcc gggcgcgacg cgacatttcc cgggcgaatg
     7321 ggggcccttc ggatcccggt ccggcggatt tccgccgacg cgccgggggg caaccaacac
     7381 ctggcgctga ccggaatcag agtcgcctgc aaatgctggc cgggatggta ttcgccaatc
     7441 ggccgtggcg cttggtcccg tcgctgcaga gcgctaccgc tgcagccgcc gccgctgcag
     7501 gcctgacgat cttttactcc tccatctggc aaatggcc
//
LOCUS       DASYLX010000356         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_16341, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000356 DASYLX010000000
VERSION     DASYLX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16341"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VGP27_08680"
     CDS             <1..1195
                     /locus_tag="VGP27_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7851423.1"
                     /translation="STTRAAHSLAEGLLLSLDQPFPVAVARLGQAITTEQQQPGVVPD
                     SPAALVTLAALHGGDPVRARSVIGRAVRAGGEDDAFSAHRHRLLLGWVRMQDGQLGPA
                     MADAGSVGDAALHRRDALWAAALNTAIARRNGDGGAVQKHWYAAMEVLAEYSVDLFAL
                     LPLGELWVAAARMRQVDRLQHALDEAFALLTSLGSPVLWSVPLHWAGVHAGILANSPE
                     AVAPHGHALTAAAGSSAFAKALSGAGRTWLRVLANHVDGDEVTAAARSLSQFGLTSDA
                     TRLAGQAALQTPDGRVSGAMLQLARDLKLTTAVDDGPGVESMPTAPAPAGSGRQQASR
                     LSDREREVAELLLLGMPYRDIGSQLFISAKTVEHHVARIRRRLGADSRSEMLSMLRAM
                     LATPG"
     gene            1346..3292
                     /locus_tag="VGP27_08685"
     CDS             1346..3292
                     /locus_tag="VGP27_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7851424.1"
                     /translation="MSSPQVGRGNSLGLTVGATNLACTGDGHRPLIRPSVVTLEPGLT
                     LTGFVDRVGDPVPMVAQDGSRHRPELLLAEALDDVARNAAKAPVSEVAVAVPAHWRAS
                     MVEALRTALRSKPGLTGAPLVSDAAAALTALRSNPGLPSAGVIVLCDFGGSGTSITLV
                     DAGANDTPIGETVRVQDFSGDQIDQAVLTRVVAGIFEASSADPAGTSMVGSLGRLRDE
                     CRRAKERLSDETATAIVVDVPGLETTVRMTRMELDDLVDEPLTEFVAVLSDTLERNRI
                     GLASVSAVATVGGGARIPLITQRLSEHLRTTVVTTPSPQLTAAQGVALIAHRSQIVET
                     ATTVAPAAPATATVAAAAFGAGGASPAVGALAWSEVADDPHDVLVTEPHFDDHYAATP
                     ADSRPEVHFQHDQREEPTPSRRRSPILLFGLAAAAAVVAAVVFGLTAFTRDTSTVPAG
                     TTSSIAPAPAPVAEPAAPAQQAPAPAQAQAPVVTTVLARPQPRVAPGAQQQAPAVQPA
                     PEWTAPAPAPEAPAPQPAPAPAPAPAPAPAPVPAPAPAPAPAPENPAPPTNPGTGGGG
                     TGTGGTGTGTGGTGTATGGTGTGTGTGTGGTGTATGGTGTGTGTGTGTGTGTGTGTGG
                     AETAPANPGLVAPILAPVLAPLPW"
     gene            3416..6106
                     /locus_tag="VGP27_08690"
                     /pseudo
     CDS             3416..6106
                     /locus_tag="VGP27_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
BASE COUNT         1046 a         2168 c         2077 g          901 t
ORIGIN      
        1 gtcaaccacg cgggccgcgc acagcctggc cgagggcctg ctgctctcac tggatcagcc
       61 gtttccggtc gccgtggccc gcctcgggca ggccatcacc accgaacaac aacagccggg
      121 cgtcgtaccg gacagtccgg ccgcactggt cacgctggcg gctctgcacg gcggcgatcc
      181 ggtccgcgcg cgcagtgtga tcgggcgtgc ggtgcgcgca ggcggcgagg acgacgcgtt
      241 ttcggcccat cggcaccggc tgctgctcgg ctgggtgcgg atgcaggacg gccagctggg
      301 tccggctatg gccgacgccg ggtccgtcgg tgacgccgcg ctgcaccgcc gcgatgcact
      361 gtgggcggcg gcactgaaca cggccattgc ccgccgcaac ggtgacggcg gcgctgtcca
      421 gaagcactgg tacgccgcca tggaagtgct cgccgagtac tccgtcgacc tcttcgcgct
      481 gcttccgctc ggcgagttgt gggtcgccgc cgcgcgcatg cgccaggtcg accggcttca
      541 gcacgcgctg gacgaggcgt tcgcgctgct cacctccttg ggcagcccag tgctgtggtc
      601 ggtgccgctg cactgggccg gcgtccacgc cggaatcctg gccaactctc cggaggcggt
      661 cgcgccgcac ggccatgccc tgacggctgc tgcaggcagc agtgcgttcg cgaaggcgct
      721 ctcgggtgcg ggccggacat ggctgcgggt gctggccaac catgtcgacg gcgatgaggt
      781 caccgccgcg gctcgctcgc tgtcacagtt cggccttacc tcggacgcca ccaggttggc
      841 cggccaggcg gcgctgcaga cgcccgacgg tcgggtgtcc ggtgccatgc tgcagctggc
      901 ccgcgacctc aagctgacca ccgccgtcga cgacgggccg ggcgtcgaat cgatgccaac
      961 ggcgccagcg ccggcgggct cgggccgcca gcaggcgtca cggttgtccg accgcgaacg
     1021 cgaggtcgcc gaactgttgc tgctcggcat gccgtaccga gacatcggca gccagctctt
     1081 catctcggcc aagaccgtcg agcaccatgt ggcgcgcata cgccgcaggc tgggcgcgga
     1141 ttcccggtcg gaaatgctgt cgatgctgcg ggccatgctg gccactcctg gctgacccgc
     1201 gaatccccta acgcattcga atcccctaat cgagatcccc caaggggccc gcacgggcac
     1261 aggggtgagc accgatccgc cgccgcttcc tgcgccatag ggtcgatcca ggcgtttcgt
     1321 taccgatccg aggtagaggt gtggcatgtc gagtccgcaa gtcggcaggg gtaattcgct
     1381 tggcctgacc gttggcgcca cgaatcttgc ctgcaccggc gacgggcatc gaccgctcat
     1441 tcgcccctcg gtcgtcaccc tcgaaccggg tctgacgctg accgggttcg tcgaccgcgt
     1501 cggcgatcct gtgccgatgg tcgcccagga cggttcccgg catcgcccgg agctgctgct
     1561 cgcagaggcg ctcgacgatg tcgcacgcaa tgccgccaag gcgcccgtgt cggaggtggc
     1621 cgtcgcggtg cctgcgcact ggcgcgcatc catggtcgag gcgctacgga ctgcgctgcg
     1681 cagcaagccc gggctgacgg gggcaccgct ggtgtccgac gccgccgcgg cgttgacggc
     1741 gctgcggtcg aatccgggac tgccgtcggc gggcgtgatc gtcctgtgcg acttcggcgg
     1801 cagcggcacg agcatcacgc tggtcgacgc gggagcgaac gacaccccga tcggcgagac
     1861 ggtccgcgtg caggacttct cgggcgacca gatcgaccag gcagtcctga caagggtggt
     1921 ggcggggatc ttcgaagcgt cgagcgccga cccggcgggc acgtcgatgg tcggatcgct
     1981 tggcaggctg cgcgacgagt gcaggcgcgc caaggagcgg ctgtcggacg agacggccac
     2041 cgcgatcgtc gttgacgtgc cggggctgga gaccaccgtt cggatgaccc ggatggagct
     2101 cgacgacctt gtcgacgagc cgctgaccga gttcgttgcg gtcctctccg acacgctcga
     2161 gcgcaaccgc atcggattgg cgagcgtgtc ggccgtcgcg acggtcggtg gcggcgctcg
     2221 cattccgctg atcacgcagc gactctccga gcacctccgc acgacggtcg tcaccacgcc
     2281 ttcgccacag ctgacggctg cccaaggcgt tgcgttgatc gcacaccgga gccagatcgt
     2341 cgagacggcg acaacggtgg cgcctgctgc acccgcaacg gccaccgtcg ctgcggcggc
     2401 gttcggtgcg ggcggcgcgt cacctgcagt cggtgcgttg gcctggtccg aggtcgcaga
     2461 cgatccgcat gacgtcctgg tgaccgaacc gcacttcgac gaccactacg cggcaacacc
     2521 tgccgacagc agaccggagg tgcacttcca acacgaccag cgggaagagc cgaccccgtc
     2581 tcggcggcgc agtccgatcc tgttgttcgg actggctgcg gcggcggccg tcgtcgccgc
     2641 ggtggtgttc gggctgacgg cgttcacccg ggacacctcc accgtgccgg ccggtacgac
     2701 gtcgagcatc gcaccggcac cggcacccgt cgcagagccc gcggccccgg cgcagcaagc
     2761 ccccgcgccc gcgcaggcgc aggcacctgt ggtgacgacc gtgctggcac ggccgcagcc
     2821 gcgggtggcg ccgggcgcac aacaacaggc accggcggtc cagcccgcac cggagtggac
     2881 ggctcccgcc cccgcgccag aggctcccgc tcctcaacct gctcccgcac cagcacctgc
     2941 acccgcaccc gcacccgcac ccgtacccgc acccgcaccc gcacccgcac ccgcacccga
     3001 aaaccctgca ccccccacca acccgggcac gggcggcggc ggcaccggaa ccggtgggac
     3061 gggaaccggc acgggcggaa cgggcacggc gaccggcggg accgggacgg gaaccggcac
     3121 cggcacgggc ggaacgggca cggctaccgg cgggaccgga acgggaacgg gaaccggaac
     3181 cggaacgggc acgggtaccg gtaccggtac cggcggcgcg gagacggctc cggcaaatcc
     3241 cggactggtg gcgccgatat tggcgcccgt gctggcgccc ctgccctggt aaggcgaacc
     3301 ttcgtagcga gctttcccgg cgagatgcaa ctatgagcag gcatcgagca taagttactg
     3361 acgggtaact tcagataagt tactcttggg taacttagat atcgggaggc acggggtgga
     3421 cacccatacg ctgatcaggc tcgtcgtcgg actcgggatg accgcgcttg tactggtcct
     3481 cgccgctcgc cgcgtcctct ggttgacgac cctgatccgg tccggccaga aaacaagcga
     3541 cgagcatggc cgcaaagacg acgtcggcac ccgcatcacc actcaggtga aagaagtctt
     3601 cggacagacc cgactgctca agtggtcgat ccccggcctg gcgcacttct tcacgatgtg
     3661 gggcttcttc atcctcgcga cggtttacct cgaggcttat ggcctgttgt tcgtccacga
     3721 cttccacatt ccgatcatcg gccgctggga cgccctcggc ttcctgcagg acttcatcgc
     3781 acttgcggta ctggcaggca tcaccacctt cgcgatcatc cgcatcgtcc gcgaacccaa
     3841 gaagctcggc cgcgagtccc ggttctacgg ttcccacacc ggtggtgcgt ggctcatcct
     3901 gttcatgatc ttcaacgtca tctggacgta tgcactcgtc cgcggctcgg cggtcaacac
     3961 caacaacctg ccctacggca agggcgcctt cttctcgaac ttcatgggct gggtcctgag
     4021 cccgctcggc cacaccgcca acgagtggct cgaaaccgtt gcgctgctgc tgcatatcgg
     4081 cgtgatgctg atcttcctgc tgatcgtgtt gcactcgaag cacctgcaca tcggcctcgc
     4141 cccgctcaac gtcaccttca agcggttgcc cgacgccttg ggcccgctgc tgccggtgga
     4201 gttcaagggc gagccgatcg acttcgagga tccgccagag gacgccgtgc tcggccgcgg
     4261 gaagatcgag gacttcacct ggaagggcta cctcgacttc accacgtgta cggagtgcgg
     4321 tcgctgtcag tcgcagtgcc cggcatggaa caccggcaag ccgctgtccc ccaagctcgt
     4381 catcatgaac ctgcgggatc acttgttcgc gaaggcaccg tacatcctcg gcgacaagga
     4441 aactccgctg gagaacaccc ccgagggcgg tctcgccgaa gagctcgaga agcacgacga
     4501 ggaacaccat gttcccgagt cgacgtttga gcgagtcctc ggttcgggac cgttgcaggc
     4561 cacccgccca ctggtcggca ccgccgagga gctcggcgtc atcgacccgg acgtgctgtg
     4621 gtcctgcacc acctgcggcg cctgcgtgga gcagtgcccg gtggacatcg agcacatcga
     4681 ccacatcgtc gacatgcgcc gctatcaggt gatgatggag tcggagttcc ccggcgaact
     4741 cggagtgctg ttcaaaaacc tcgaaaccaa aggcaatccg tggggccaga acgccaagga
     4801 ccggaccaac tggatcgacg aggtcgagtt cgacgttccg gtgttcggca aggatgtcga
     4861 ctcgttcgaa ggttacgagt acctgttctg ggtgggctgc gccggcgcct atgaggaccg
     4921 cgccaagaag accaccaagg ccgtcgccga actgctcgcg accgccggag tgaagtacct
     4981 ggtgctcggc gagggcgaaa cctgcaacgg tgactccgcc cgccgctccg gcaacgagtt
     5041 cctcttccag caactcgccg cgcagaacgt cgaaaccctc aacgatctgt tcgaaggtgt
     5101 cgaacgggtc gaccgcaagg tcgtcgtcac ctgcccgcac tgcttcaaca cgctcggccg
     5161 cgaatacccg caggtgggcg gcaattacac ggtgttgcac cacacgcagt tgctgaaccg
     5221 gctgatccgg gacaagaagc tggttccggt caagccggtg gacggcgaag actcatccat
     5281 cacctatcac gacccgtgct acctcggccg gcacaacaag gtctacgacg ctccgcgcga
     5341 actcatcggg gcgtccggcg ccaagctcac cgagatgccg cggcacgccg accgcggatt
     5401 gtgctgcggt gcaggcggtg cgcggatgtg gatggaagag cacatcggca agcgggtgaa
     5461 ccacgagcgc gtcgaggagg cgctggacac cggcgcgtcg aagatcgcca ccggctgccc
     5521 gttctgccgc gtgatgatca ccgacggcgt cgacgacgtc gccgcagcca atgatctgcc
     5581 caaggccgag gttctcgacg tggcgcagct gctgctcggc tcgctcgaca agagctcgat
     5641 caccctgccc gaaaagggca ccgccgcaaa ggaacccaag aggcgggcag ccagggtcga
     5701 ggcgtctacc gcgacggtcg atgagaccgc gcccgccacg acccaggagg agccgacggc
     5761 gacggccgcc cccgccccgg agaagcctgc agctgccgcg cccgtcaagg gactcggcat
     5821 cgccggcggt gcgaagcgcc ccggcgcgaa gaaggccgcg gcagcgcctg ccgaaccggc
     5881 ggcgaaggag gccaagcccg cagaggccgc tcctgccaag ggtctcggca tcgcggcggg
     5941 agccaagcga ccgggtgcga agaagaccgc agccgcgccc gccgaggcga aggccgagcc
     6001 gaaggcggag gcccctgcag aggcgaccgc cgaggcaccc gccaaacccg aagccgaggt
     6061 caagggactg ggcatcgccg cgggcgcacg ccgcccaggg gccaagaagg ctcccgcgaa
     6121 gcctgcgccg aacgagggcg aggccaccgt cacgcagccg cccaacgtcg atcccgacaa
     6181 ggcggagcca gg
//
LOCUS       DASYLX010000357         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_16377, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000357 DASYLX010000000
VERSION     DASYLX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16377"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="ilvC"
                     /locus_tag="VGP27_08695"
     CDS             complement(<1..927)
                     /gene="ilvC"
                     /locus_tag="VGP27_08695"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV7851425.1"
                     /translation="MFYDDDADLSIIQGRKVGVIGYGSQGHAHSLSLRDSGVEVKVGL
                     KEGSKSRAKVTEQGLEVDTPAEVAKWADVIMLLAPDTAQGEIFTADIEPNLTDGNALF
                     FGHGLNIHFGLIKPPANVTVGMVAPKGPGHLVRRQFVDGKGVPCLVAVEQDPKDEGQA
                     LVLSYAKGIGGTRAGVIKTTFKDETETDLFGEQAVLCGGTEELVKTGFDVMVEAGYAP
                     ELAYFEVLHELKLIVDLMYEGGIARMNYSVSDTAEFGGYLSGPRVIDADTKQRMKQIL
                     SDIQDGSFVKKLVANVEGGNKQLEDLRKQNAEH"
     gene            complement(981..1487)
                     /gene="ilvN"
                     /locus_tag="VGP27_08700"
     CDS             complement(981..1487)
                     /gene="ilvN"
                     /locus_tag="VGP27_08700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV7851426.1"
                     /translation="MSSTPTQTLSVLVEDKPGVLARIASLFSRRGYNIQSLAVGATEQ
                     KNMSRMTIVVSVDEHPLEQITKQLNKLINVIKIVEQEEDNSVSRELALIKVRADAATR
                     GQVIEAVNLFRAKVVDVSTESLTIEATGTPAKLEAFLRVLEPYGIRELVQSGVVSVSR
                     GPRGIGAK"
     gene            complement(1487..>2059)
                     /locus_tag="VGP27_08705"
     CDS             complement(1487..>2059)
                     /locus_tag="VGP27_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7851427.1"
                     /translation="QHQMWAAQFISYENPRTWLNSGGLGTMGYAVPAAMGAKFARPDA
                     EVWAIDGDGCFQMTNQELATCAVEGAPIKVAVINNGNLGMVRQWQTLFYEKRYSQTDL
                     ATHSHRIPDFVKLAEALGCVGLRCERAEDVADVINQARAINDRPVVIDFIVGADAQVW
                     PMVAAGTGNDEIMAARGIRPLFDDPEEGHA"
BASE COUNT          337 a          690 c          623 g          409 t
ORIGIN      
        1 gtgctcggcg ttctgcttgc gcagatcctc gagctgcttg ttgccgccct ccacgttggc
       61 gaccagcttc ttgacgaagc tgccgtcctg gatatcggag agaatctgct tcatccgctg
      121 cttggtgtcg gcgtcgataa cccgcggacc cgacaggtag ccgccgaact ccgcggtgtc
      181 ggacaccgag tagttcatcc tggcgatgcc gccctcgtac atcagatcga cgatcagctt
      241 cagctcgtgc agcacctcga aatacgccag ttccggcgca tatccggcct ccaccatcac
      301 gtcgaagccg gtcttcacca gctcctcggt gccgccgcac agcaccgcct gctcgccgaa
      361 caggtcggtc tcggtctcgt ccttgaacgt ggtcttgatg acgccggcac gagttccgcc
      421 gatgcccttg gcgtaggaca gcacaagcgc ttggccctca tccttgggat cctgctcgac
      481 ggcgacgagg cacgggacac ccttgccgtc gacgaactgc cgacgcacga ggtggccggg
      541 tcccttcggg gcgaccatgc cgacggtgac gttggcgggc ggcttgatca gaccgaagtg
      601 aatgttgagg ccgtgcccga agaacagcgc attgccgtcg gtgaggttcg gctcgatgtc
      661 tgctgtgaag atctcaccct gtgcggtgtc gggcgccagc aacatgatga cgtcggccca
      721 cttcgccacc tcggcggggg tgtccacctc gaggccctgc tcggtgacct tcgcccggga
      781 cttcgagcct tccttgagac cgaccttcac ctcgacgccg gaatcgcgca ggctcagcga
      841 gtgcgcatgg ccctggctgc cgtagccgat gacgccgacc ttgcgtccct ggatgatcga
      901 caggtccgcg tcgtcgtcat aaaacatctc aactgccact gtatttcctt ctcttctcac
      961 tcatttaagg gcttactggc ttacttggcg ccgatgccac gcggaccgcg cgacaccgac
     1021 accactccgg actgcacgag ctcacggatg ccgtatggct cgagcaccct caggaacgct
     1081 tcgagcttcg ccggcgtacc ggtcgcttcg atcgtcagcg attcggtcga cacgtcgacg
     1141 accttcgcgc ggaacaggtt caccgcttcg atgacctgac cgcgagtggc ggcatcggcg
     1201 cgcaccttga tcagcgccag ttcccgcgac accgagttgt cctcctcctg ctcgacgatc
     1261 ttgatcacgt tgatcagctt gttgagctgc ttggtgatct gttcgagtgg atgctcgtcg
     1321 acactgacta cgatggtcat ccgagacatg ttcttctgct ccgtggcgcc gacggccagg
     1381 gactggatgt tgtagccgcg ccgcgagaac agcgaagcaa tccgggcgag aacgcccggc
     1441 ttgtcctcga cgagcaccga aagcgtctgc gtcggagtcg aactcatcac gcgtgccctt
     1501 cctcgggatc gtcgaacagc gggcggatgc cgcgggcggc catgatctcg tcgttgccgg
     1561 tgcctgcggc caccatcggc cacacctgcg catcggcgcc gacgatgaag tcgatcacca
     1621 ccggccggtc gttgatggcg cgggcctggt tgatcacgtc ggcgacgtct tcggcacgct
     1681 cacaacgcaa tccgacgcag cccagcgcct cggcgagctt gacgaagtca gggatgcgat
     1741 gcgaatgcgt ggccagatcc gtttgactgt accgcttttc gtagaacagc gtctgccact
     1801 ggcgcaccat ccccaggttg ccgttgttga tcacggcgac cttgatcggt gcgccctcga
     1861 ccgcgcaggt ggccagctcc tggttggtca tctggaagca gccgtcgccg tcgatggccc
     1921 acacctcggc gtcggggcga gcgaacttcg cacccatcgc ggcgggcacc gcgtagccca
     1981 tcgtgccgag tccgcccgaa ttgagccatg tccgcgggtt ctcgtatgag atgaactgcg
     2041 cggcccacat ctggtgctg
//
LOCUS       DASYLX010000358         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2101005,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000358 DASYLX010000000
VERSION     DASYLX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101005"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGP27_08710"
     CDS             complement(<1..218)
                     /locus_tag="VGP27_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEV7851428.1"
                     /translation="MDVDAFVLAHRQTWNRLDELVKRRRRLTGAEVDELVELYQRVST
                     HLSMVRTSSSDSVLVGRLSSLVARARSAV"
     gene            246..1106
                     /locus_tag="VGP27_08715"
     CDS             246..1106
                     /locus_tag="VGP27_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7851429.1"
                     /translation="MVGQAEQVVTGDAVVLDVQIAQLPVRALSALIDIAVIFVGYMAG
                     MMLWAVTLSTFDPALSGAVLVIFTVLTLIGYPVIFESATRGRTLGKMALGLRVVSDDG
                     GPERFRQALFRGLASFVEIWMFFGGPAVICSLLSPKGKRLGDVFAGTVVISERGPKLP
                     PPPVMPPSLAWWASSLQLSGLGPEQAELARQFLARAPQLDPQIRDQMAYRIAGEVVTR
                     ISPPPPPGVPPQYVLAAVLAERHRRELERLRPAHPEPPAWAARAQWVPPPGSPGPPPA
                     SPGGGFAPPS"
     gene            complement(1112..1606)
                     /locus_tag="VGP27_08720"
     CDS             complement(1112..1606)
                     /locus_tag="VGP27_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEV7851430.1"
                     /translation="MNTSPMVILTSVAAIASAAAAGMMYVFSTFVMRGLDRTGPVSAI
                     AAMRGINVEANTNAPFLLAYFGAALLAVAVGVVAVVQIRQPGSGWLLAGAIFGVLGAI
                     ITIAFNVPLNNHLDTVDPSGLSVADAAREWHAYFSAWTVWNHARTTTAVIAAALMLWG
                     LRYR"
     gene            1709..2665
                     /locus_tag="VGP27_08725"
     CDS             1709..2665
                     /locus_tag="VGP27_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7851431.1"
                     /translation="MDAVAGLLDGVRARGAFVLRMMLDPPWSMRIQDEAPLTLICQTR
                     GRAAIVGDTSGTVWLDPGDVALTRGTEHYLFADDPETRPMIVIHPGQRCTTRSGDDLR
                     FEMSLGVRTWGNSSSGSDRSIVCAYEGHSEVSGRLLDALPAVLVLRAHEWDSPLIDLL
                     AAEAGRDGSGQEAYLDRLLDLLLIAVLRIWFDREENAPPWWHAEHDTMVGPALRLIYN
                     NPAHPWTVANLAAAVGTSRAVFARRFTELVGEPPIAFLTGWRLALAADLLRTSSATIA
                     SVARQVGYSTPFALSSAFKRAYGVSPKTHRATAGPLPDVASR"
     gene            2735..3463
                     /locus_tag="VGP27_08730"
     CDS             2735..3463
                     /locus_tag="VGP27_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV7851432.1"
                     /translation="MNDPFTPTPGGFGFGFGPEQRRALHEQRRHARRDFREHIREHGT
                     DRHGPFGPGFQAGFGGFGPGRGGFGFDPRAGFGFGGGPRGRRGGGRRGKRGDVRAAIL
                     VLLSDRAMHGYEMIGEISDRSGGLWRPSPGSVYPTLQLLVDEGLIVGTESDGSKRLFE
                     LTDDGRAAAEKVETPPWEEIAEGWKDQAPGQVNLRTATGQLMGAVAQSAYAATEEQQQ
                     RIVDIVNNARREVYNILGETDETE"
     gene            3513..>3656
                     /locus_tag="VGP27_08735"
     CDS             3513..>3656
                     /locus_tag="VGP27_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851433.1"
                     /translation="MQTFPLGSFHVGRVGFGAMQLPGPGVFGPPRDRDQAIAVLRRAL
                     ELGI"
BASE COUNT          578 a         1234 c         1230 g          614 t
ORIGIN      
        1 accgccgaac gggccctggc caccagcgac gacagccggc ccaccaacac cgaatcgctg
       61 gatgacgtcc gcaccatcga caggtgcgtc gacacgcgct gataaagctc gacgagctcg
      121 tcgacctcgg cgcctgtcag ccgcctgcgc cgcttgacca gctcgtcgag ccggttccac
      181 gtctgccgat gggccagcac aaacgcgtcg acatccacct ggcaaatcct agtagcctgg
      241 cgggaatggt cggccaggca gaacaggtgg tgaccggcga cgccgtcgtc ctggatgttc
      301 agatcgccca gctgcctgtg cgggcgctgt cggcgttgat cgatatcgcg gtgatcttcg
      361 tcggctacat ggcgggcatg atgctgtggg cggtgacgct gagcacgttc gacccggccc
      421 tctcgggggc ggtgctcgtc atcttcaccg tgctcacgct gatcggctat ccggtgatct
      481 tcgagagcgc aacccgtggc aggacgctgg gcaagatggc gctcggcctt cgggtggtgt
      541 ccgacgacgg cggcccggaa cgattccggc aggcactgtt tcgcgggctg gcgtcgttcg
      601 tcgagatctg gatgttcttc ggcggccctg cggtgatctg cagcctgctg tcgccgaagg
      661 gcaaacgcct cggcgatgtg ttcgcgggca ccgtggtgat cagtgaacga gggccaaaac
      721 tcccgccgcc gccggtgatg ccgccgtcgc tggcgtggtg ggcctcgtct ctgcagctgt
      781 ccggcctcgg ccccgagcag gctgagttgg ctcgtcaatt cctcgctcgc gcaccgcaat
      841 tggaccctca gatccgcgac cagatggcgt accgcatcgc cggtgaggtg gtcacgcgga
      901 tttctccgcc cccgccgccc ggcgttcctc cgcagtacgt actcgccgct gtgcttgcgg
      961 agcggcaccg gcgggaactg gaacggctgc ggcctgcaca tccggagccg ccggcgtggg
     1021 ccgctcgggc gcagtgggtg cccccacccg gctcaccggg gccgccgccc gcttctcccg
     1081 ggggcggctt cgcgcctccc agttagccgt ctcagcggta ccgcagcccc cacagcatca
     1141 acgcggctgc gatgacggcg gtggtggtgc gggcgtggtt ccacaccgtc cacgccgaga
     1201 agtacgcatg ccattcgcgg gcggcgtcgg cgacggacag ccccgagggg tcgacggtgt
     1261 cgagatgatt gttgagcggc acattgaagg cgatggtgat gatcgcgccg agcaccccga
     1321 atattgcgcc cgccaacagc catccgctgc ccggctgacg gatctggacg actgccacca
     1381 ccccgacggc gactgccagc agtgcggccc cgaaataggc cagcaggaag ggcgcgttgg
     1441 tgttcgcttc gacattgatg ccgcgcatcg cggcgatcgc actgaccgga ccggttcgat
     1501 ccagtccccg catcacgaag gtggagaaga cgtacatcat ccccgcggcg gccgcgctgg
     1561 cgatcgcggc gacggacgtg aggatgacca ttgggctcgt gttcatgact aagagtcaac
     1621 gggtcgatgt cgagacgatc catcgctgac tgtcccaatc gcatacgcat tcgtctcgaa
     1681 tcggcttgac cggcggtagc ctcggggcgt ggatgccgtg gccggactgc tcgacggagt
     1741 gcgggcgcgc ggtgccttcg tgttgcggat gatgctggat ccgccgtggt cgatgcgcat
     1801 tcaagacgag gcgccgctga ccttgatctg ccagacccgc ggtcgggcgg ccatcgtcgg
     1861 cgacaccagc ggaaccgtct ggctcgaccc aggcgatgtc gcgctgaccc gcggcaccga
     1921 gcactacctg ttcgccgacg atcccgagac acggccgatg atcgtcatcc atcccggtca
     1981 acgctgcacc acgcgctccg gcgacgacct ccgtttcgag atgtcgctcg gggtgcggac
     2041 atggggcaac agctccagcg ggtccgatcg gtcgatcgtc tgcgcctacg agggccatag
     2101 tgaggtcagc ggccgactgc tcgatgccct gccggccgtg ctggtgctgc gcgcacacga
     2161 gtgggactcc ccgctgatag atctgctcgc cgcggaggcg ggtcgcgatg gttcaggcca
     2221 ggaggcctat ctcgacaggc tgctcgacct actgctgatc gcggtgctgc gcatctggtt
     2281 cgaccgcgag gagaacgccc cgccgtggtg gcacgccgaa cacgacacga tggtcgggcc
     2341 ggccttgcgg ttgatctaca acaaccctgc gcatccgtgg actgtcgcga atcttgctgc
     2401 agcggttggt acttcgcgtg cggtgttcgc acgtcgattc accgaactgg tcggcgagcc
     2461 gccgattgcg tttctcaccg gctggcgcct ggcgctcgcg gcggatctcc tgcgaaccag
     2521 cagcgcgacg atcgcctcgg tggccaggca ggtcggctac agcacgccgt tcgcattgag
     2581 cagtgcgttc aagcgggcct acggtgtcag cccgaaaacg caccgggcca ccgccgggcc
     2641 actgccggac gttgcatcac gatagtttcc gatatatcgt tgacgtatcg gaagtgcaca
     2701 gggcgcttcc tgacgaaggt aaggactttc tccaatgaat gacccattca cgccaacacc
     2761 cggcgggttc ggcttcggct tcggtccgga gcagcgccgc gccctccacg aacagcggcg
     2821 gcacgcccgt cgcgatttcc gcgaacacat ccgtgagcac ggcaccgatc ggcacggtcc
     2881 gttcggcccc ggcttccagg ccggcttcgg cggcttcggg cctggccgcg gcggcttcgg
     2941 cttcgatccg cgcgcaggtt tcggtttcgg cggtggcccg cgtggccgtc gcggcggagg
     3001 ccgacgaggc aagcggggcg acgtccgtgc cgccatcctg gttctgctgt ccgaccgcgc
     3061 catgcacggc tacgagatga tcggagagat ctccgatcgc agcggcggcc tgtggcgacc
     3121 cagcccgggc tcggtctacc cgaccctgca actgctggtc gacgagggcc tcatcgtcgg
     3181 caccgaaagc gacggcagca agcgtctttt cgagctgacc gatgacggcc gcgccgcggc
     3241 cgagaaggtc gagacgccgc cgtgggaaga gatcgccgag ggctggaagg atcaagcccc
     3301 cggccaggtc aacctgcgca ccgctaccgg ccagctgatg ggcgccgtgg cccagtcggc
     3361 gtacgccgcg accgaggagc agcagcagcg catcgtcgac atcgtcaaca atgcccgccg
     3421 cgaggtgtac aacatcctcg gcgaaaccga cgaaaccgag tagcaaagat ccagtcggaa
     3481 taagcccggc gggaatggtg tacgcactaa ctatgcaaac cttcccgctg ggctctttcc
     3541 atgtcggccg cgtcggattc ggcgccatgc agttgcccgg ccccggtgtg ttcggtccgc
     3601 cgcgcgaccg ggaccaggcg atcgcggtcc tgcgccgagc cctcgagctg ggcatc
//
LOCUS       DASYLX010000359        22958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2977768,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000359 DASYLX010000000
VERSION     DASYLX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22958
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977768"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VGP27_08740"
     CDS             complement(<1..1527)
                     /locus_tag="VGP27_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV7851434.1"
                     /translation="MTDNALAAPPTTDPCPRGRISTEAGRRRTFAVISHPDAGKSTLT
                     EALALHARVITEAGAVHGKAGRRATVSDWMEMEKARGISITSTVLQFPYRTCVINLLD
                     TPGHADFSEDTYRVLTAVDSAVMLIDAAKGLEPQTLKLFQVCKHRRIPIITVINKWDR
                     PGRDALELMDEIHARIGLRPTPLTWPVGIAGNFKGVLDRRTGNFIRFTRTAGGATAAP
                     EEHIAAPDAHSAAGDDWDHAVEESELLSADGSDYDREQYLNGAATPVLFTSAALNFGV
                     NQLLDVLVELAPPPSGAVDVDGAWRAAESPFSAFVFKVQAGMDSAHRDRIAYARVCTG
                     TFARGDVLTHAATGKPFVTKYAQSVFGQQRATLDTAWPGDVIGLANAAALRPGDTLYR
                     DVPVQYPPIPSFSPEHFAVARGTDPSKHKQFRRGIEQLEQEGVVQVLRSERRGDQAPV
                     LAAVGPMQFEVAAHRMATELSAPISLESLPYQVARVVDPADAEFMSKQVSAEVLTRTD
                     G"
     gene            complement(1642..2040)
                     /locus_tag="VGP27_08745"
     CDS             complement(1642..2040)
                     /locus_tag="VGP27_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851435.1"
                     /translation="MDDGTATSEHDDVKENLLIEGLRDWIRLGEIHGTFLFDNYTSRR
                     PVAEAQQLTLSMIRELVDDGLFDLGEIVRNTNDGFEAWNMSLDEAMAKIEERYVTNFD
                     DRWGWTACAWLQLTENGRALALQLYHAEDR"
     gene            2882..3511
                     /locus_tag="VGP27_08750"
     CDS             2882..3511
                     /locus_tag="VGP27_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851436.1"
                     /translation="MGTEPDVKEIWDESSMKRLWDYLTRNANDGVPPPDYRGPVRVLP
                     DGTRIGYRGSGRWGDTIDVWYPDAYLKGHTPYEPYFPPLVSGPPQLPPAASIPSVQVM
                     PPQLTHPPTTLPPAGIFEPNGLPPWLQNPSAPGLHSAAQSPTIMPGVALPDTPQASAP
                     APDDGPGLSEMGGTFVDTGEAVGSLIVGGIVIVGGLLGTVATPSGQIAR"
     gene            complement(3539..3937)
                     /locus_tag="VGP27_08755"
     CDS             complement(3539..3937)
                     /locus_tag="VGP27_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851437.1"
                     /translation="MDDDKATSEHEDVKERLLIEGLQDWIHLTEIHTEFAFDNQTPKR
                     PVAEAQQLTLNMIRELAEEGLFDLGEIVRKGHSGFEAWNLPLDEAMAKIEHAYVTNFD
                     ERWNWSFYVWLQLTEKGKALALRLYHADDP"
     gene            complement(4038..4436)
                     /locus_tag="VGP27_08760"
     CDS             complement(4038..4436)
                     /locus_tag="VGP27_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851438.1"
                     /translation="MDDDVGASEHDDVKERLLIEGLQDWIHLSEIHQAFAFENHAPKR
                     PVAEAQQLTLNMIRELVNEGLFVLGEPDNKRPSGFESWGVSLDEAMAEIQHKYVKNFD
                     DRWNWVTCAWLELTERGKALALKLYHADEP"
     gene            complement(4491..6092)
                     /locus_tag="VGP27_08765"
     CDS             complement(4491..6092)
                     /locus_tag="VGP27_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851439.1"
                     /translation="MTSATPVDAGSTWAPLRSPVFRALWIAQFVSNLGTWMQTVAAQW
                     MLVGDPRAAVLVPLVQTATTLPVMLLALPSGVLADLVDRRRLLIATQAAMAAGVALLA
                     SLTGAGLTTPTVLLLLLFLIGCGQALTVPAWQAIQPDLVSPEQIPAAAALGSMSVNGA
                     RAIGPAIAGALVSLAGPTVVFALNAVSFAGIVIVLVVWRKPAIEHRFPAERALAALSA
                     GGRYIRRSPIVRRILLRTALFIAPGSALWGLLPVIAKDQLGLSSSGYGLLLGALGVGA
                     VLGALVLSRLRSRFGQNALLTVAAAGFAASTVVLALVHNFVAVLVGLAFGGAAWLLYL
                     STLNASMQLSLPGWVRARGLSVYQLIFMGGQALGSLVWGVLAGATSSVVSLLVSAGLL
                     AVCALSTLWWPLHARTGNLDLTPSSHWPEPTLVFEPEPLDGPVLVMTTYRVEPKDEAA
                     FLTAIAAMGRSRQRTGAARWRLYRSVEREHTFVETFIVRSWGEHLHQHYTRLTGNDLL
                     IEQAVEQFLDHEPVSHHLIAVHDVG"
     gene            complement(6290..7372)
                     /locus_tag="VGP27_08770"
     CDS             complement(6290..7372)
                     /locus_tag="VGP27_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0565 family glycosyltransferase"
                     /protein_id="HEV7851440.1"
                     /translation="MRVALITYSTKPRGGVVHTLNLAEALADFGIDVSVWSLARQGDT
                     GFFRAVDPRVAVRLVDFPEVENEPVTERIVRSIETLRGDFVADDYDIVHAQDCISANA
                     VGPCIRTIHHLDEFTTPILVACHEKAVVEPYARICVSAAVAAEVVAGWGLHPTVIPNG
                     VRADRFIAAASDGVGKQRWRDELGRYVLAVGGIEPRKGSLDLVSSYHLFRQRFPDISL
                     VIAGGETLFDYRDYRAEFERRCSELDVAPIILGAVEDTELPSLVAASEVFGFPSTKEG
                     FGLAAMEALAAGRPVVTRNLPVLREVFGEAVRYANDPLGFAAAMATSLGDDSRASAGR
                     ELALSMTWEAAARRHIEFYEAHPARS"
     gene            complement(7376..8218)
                     /locus_tag="VGP27_08775"
     CDS             complement(7376..8218)
                     /locus_tag="VGP27_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV7851441.1"
                     /translation="MAPVTVAAVAANFGRDVARCVEKVVVIVEAAAREGVQLLVFPDA
                     CLGGYIGDLRAPDPDDPPPALAPDGPEIAAVIAGAGPMTVCVGYAEAAAGGGRYNSAI
                     CVSGDGVLGTHRKVHQPAGEALAYLAGDSFSAFDTPVGRIGMLIDYDKTFPESARALA
                     LDGASIIAALSAWPASVTDRASRLPADRQSRLFDLYDCARAAENQVVLVSSNQTGVMG
                     ALQFLGQAKVVGPGGDILATTRSKGGLAKVEVDVESEISRARKVLNHLAERRLDAYRA
                     RSRS"
     gene            complement(8229..9644)
                     /locus_tag="VGP27_08780"
     CDS             complement(8229..9644)
                     /locus_tag="VGP27_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/sll0787 family protein"
                     /protein_id="HEV7851442.1"
                     /translation="MKTLAHEVIRHRELSILAGVRTHPQPGFLIHPAEGREGDEYRWL
                     RREAFVVEQGLFAGSDRDDADDDPRTVVLVAAAPDGSVLGGVRLTPRCAPDIGWWTGS
                     RLVADPSVRTSGVGAALIRAACAHVESAGALRFEATVQRRYQELFIGLGWNSVGDCDV
                     AGRHHVRMRWPLNQIQRAADATKSFLGAVLDPLRAVEGGLGPTGFVGDDGVPLPGSDI
                     VAACDAIIPSMVERDPEWAGWCSVLVNVNDLTAMGAAPVGLLDAVGAPTQSLLTRIMR
                     GVARGAQAWRVPVLGGHTQLGVPAALAVTALGRTAQPVPAGGGIAGDNLRLTADLSGH
                     WRPGYQGRQWDSTSTRSSDDLVTMAGLVDAMRARAAKDVSMAGIVGTAAMMAEASGTG
                     AELDVSAIPRPAGAEMGSWLTCFPGFGMLTVDGSPVPVPAGVASEVCGRLTVEHGVRL
                     RWPDGAVTTAVAAGLTGLGRA"
     gene            complement(9641..10696)
                     /locus_tag="VGP27_08785"
     CDS             complement(9641..10696)
                     /locus_tag="VGP27_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006360348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEV7851443.1"
                     /translation="MSVSTRIDLALLGIRGRPPVSRTAGAGPSDDGHLVIDGLNAAIP
                     RNPHSPFMFDGAQILFDGEDTGLDVQVIERPKFYDLVTDDGVPYEQLATLHGRNVLAT
                     TVVQTCIRYSPDQRCRFCSIEESLRAGATTAVKRPAELAAVAEAAVRLDGVAQMVMTT
                     GTSAGSDRGARHLARCVKAVKAAVPALPIQVQCEPPADLSVLTELRDAGADAIGIHVE
                     SLDDDVRRRWMPGKATVPLTQYREAWREAVRLFGRNQVSTYLLVGLGEDPDELVAGAA
                     ELIAMGVYPFVVPFRPHPGTLAVDVDAARAPDAALVEKVSREVAAQLRLAGMSGADQR
                     AGCAACGACGVLQNLGG"
     gene            complement(10708..11976)
                     /locus_tag="VGP27_08790"
     CDS             complement(10708..11976)
                     /locus_tag="VGP27_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7851444.1"
                     /translation="MTAHLPVAIIGAGQAGLSVSWYLSRAGIDHVVLEAKTPAHAWAD
                     SRWDSFTLVTPNWHCRLPGYSYAGPDPDGFMTRDEVVDWLAGWMDTFDAPVRAHTRVT
                     RLRNIIGGGFELTVSTDAGEETMTCDHAVVATGGYPLPVTPAFAGSLDPAVTQIHSEQ
                     YRNAGQLPQGAVLVVGTGQSGAQIAEDLHLEGRQVHLAVGSAPRVARFYRGRDCMTWL
                     SDMGLYDRAAGEYPGGKAAIEKTNHYVTGRDGGRDIDLRHFAVEGMRLYGALTDGKHS
                     SLQFAPTLRASLDHADSVYNSICADIDSYIERERISAEPPRRYTRAWQPETEPTTLDL
                     AAEGVTSIVWAIGYRPDYRWIEASAFDGGGRPMQNRGVTSVPGLSFVGLPWMHTWGSG
                     RFLGIDHDARHIATTIAATYESPLRLAASQ"
     gene            complement(11973..12269)
                     /locus_tag="VGP27_08795"
     CDS             complement(11973..12269)
                     /locus_tag="VGP27_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HEV7851445.1"
                     /translation="MPEMTFDVRWPDGSVQSCYSPSLVMHDYLSAGASYTVADFVDRS
                     GIALAEASERVRAKFGFMCTSAAATTEEIAEVASRFEPSAHVHVTAMQPPLATS"
     gene            complement(12262..13152)
                     /locus_tag="VGP27_08800"
     CDS             complement(12262..13152)
                     /locus_tag="VGP27_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV7851446.1"
                     /translation="MTTLAAVSANFTRDLEQNYALISSLAEEARENSVDFLVLPEAAI
                     GGYLSSLGNHGDTVKTSTPSLPPAIRLDGPEIAHVQAIIGDLVVAIGFCEQAEDGETR
                     YNAAAVLDGNTVYGAYRKVHQPLGEGTSYSAGSGYDVFDTPVGRIGLQICYDKAFPEA
                     ARIMALQGAQIIASLSAWPAARTATAENLQDDRWTYRFNLFDTARALDNQVFWIASNQ
                     SGTFGSLRYVGNAKVVDPGGNVLATTLLGSGMAVAEVDIDSTFRAMRGGMFHLRDRRP
                     DVYGPLTDGAVAKWRELAHA"
     gene            complement(13183..13704)
                     /locus_tag="VGP27_08805"
     CDS             complement(13183..13704)
                     /locus_tag="VGP27_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEV7851447.1"
                     /translation="MPFDDSIDENIKTSLAEIPHPALPKGSNIYGGTKIFPDYKAENG
                     ETYFTLVHGIAHESSVSFVAVLQATRALRKGFESAIYFYGPGAINCLATRGFPKTGDS
                     GFPGEQNINDSLATFIGEGGTVFCCRFGLALHGGREEDLIEGVIPAHPLDVQDAVIHY
                     ARKGAIINSTYMV"
     gene            complement(14366..15052)
                     /locus_tag="VGP27_08810"
     CDS             complement(14366..15052)
                     /locus_tag="VGP27_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7851448.1"
                     /translation="MRQPTSALAVAVICVSIAGCASTIGNAEMPTTTRSMIPRPLVER
                     ELVGLLLNPEQVNFAMGTAGMAVTNQQASMSDNSATMAPPECLAIDGAAEAPVYADSG
                     YWAERDQSLNNGDKFTHYLKQALVLFPTIEKAGAFFDASAERWPACRGYTHTQSGTQW
                     SVGQISNADGALSVTATEQDAAAPGWGCGRALTHRNNIIVDVNTCSADPADSAVKIAE
                     QLAANVTARW"
     gene            15466..16095
                     /locus_tag="VGP27_08815"
     CDS             15466..16095
                     /locus_tag="VGP27_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851449.1"
                     /translation="MGVTPADLTGTERAVLCVLMAESRPLPNPDLINLGPKLDKPSRD
                     KLNGLGLIESNRESGRFVHELTDKGWRLCREIIAAGPPPRSTGPAKTLYTVLGALGRY
                     LNGAELSLADVFGSSDGVHATASIEDRIRSAYADLAPRPGGWVSLTRLRAELSDADRG
                     DIDTALRTLYRAAGVSLIPEENQKVLTTEDRHAAVLIGDQSKHLIAIEI"
     gene            16095..19244
                     /locus_tag="VGP27_08820"
     CDS             16095..19244
                     /locus_tag="VGP27_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEV7851450.1"
                     /translation="MHEAEREALASLRLTWAPTTDDLWRPQAATHVPGLNEYAVDDVM
                     AAFGDAARDPVSAPLGVVIRGTAGTGKTHLLGQIRERVQAANGFFFVVELLDAASFWD
                     SVRSGVLESLGRPGAQRETQLKDLLWELSSIAHVSRAERRAIIGDDDLDRETLDRFVN
                     ALAKAHRDPARDYHQTLRALVLLAAADLTAHDVGEAYLQGSDESGDDGRSRWGLRGSP
                     DSAQECVKGVARLIALAGPAVLALDQIDTLLAQSLSRTDATDRDGGDAVLHQVADGLM
                     SVRQTMRRTVGVVACLPAVWEGIRHGATATVADRFRETSPLKGLPSADIGRAILERRF
                     AASYAEVGFRPLYPSWPIRPEAFADAADHTPRHLLKIADAHVRQCLKHDTVVELDRLS
                     AHWDPEPVDPIPIDPRPSELDRRFSEYRQNAVTAAALDLEGEDTAVPELLRAALTAWI
                     TERDEDSQSYTCDPPPGAHVVLHARLRQSLDPTTEDERHWAFRAIAASNAVAAQSRIK
                     KSWSVTGLGEGSNRRKLFLLRNTPWPTGPKTAALVEEFHRAGGRTVELTEDDIRTMTA
                     LRDLIGEDDHDLPGWLRARRPAHGLALLQEVLGEDGRAPRPTQRLPPAPQPVPKREPE
                     PAPAPTVVDDTPDYVPDDELPLGADVRSDATVSVELSALRKHIAIFAGSGSGKTVLIR
                     RLVEECALRGVSSIVLDPNNDLARLGTEWPQHPNGWRRSDDSRASEYLNNTEVVIWTP
                     HRNGGRPLVFQPLPDFPSVVDDADEFNDAVESAAAALEPRASIAGQSQKAHRSRAVLR
                     EALQWYGRQPTPTLGGFVDILDDPPDDLSGLADARKIASDLSQNLRASMVNDPLFGGR
                     GTPVDPAVLLTPSDGHRARVSVISMIGLSSDEQRQGFVNQLQMALFAWIKRNPAGDRP
                     LGGLLVMDEAQTFVPRQGFAACRRSTLALSSQARKYGLGLVFATQAPKSLDNQIPGNA
                     ATQFYGLLNAPAQIETAKEMARAKGGLVPDISRLRAGNFYVATEGQAFHRIVAPWCLT
                     YHPPSPLSPEEVLGLARGD"
     gene            complement(19250..19885)
                     /locus_tag="VGP27_08825"
     CDS             complement(19250..19885)
                     /locus_tag="VGP27_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851451.1"
                     /translation="MIATSFGRRALDPWVLLLSAFGGTMAWALDFPFPLVTAVVMWGT
                     AVTVGGVSWRRTLQTGTQQYALITLFDGHVRSLRALQSQALPEVVRFHADKALAAADA
                     VRPTVAQTAAAVDAMDEAIALACNVSGRGEHTVDSIGRTVGRLQSRRDGLVEKLTAAV
                     DEVATVYAGLLELSATARTMGVALDDGDVGAVNDSVTLLQMTFAELEADAA"
     gene            complement(19885..21654)
                     /locus_tag="VGP27_08830"
     CDS             complement(19885..21654)
                     /locus_tag="VGP27_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding and VWA domain-containing
                     protein"
                     /protein_id="HEV7851452.1"
                     /translation="MATNGRALLAAAVAGLMVVGVAYWWKQHDDNAAGPRAGCTTVVV
                     AASVEKSDLMDEAAKRYNTSSRLADGHCYGIAIASMTSGLAESRLAEGRWEPTWGPAP
                     DAWSPAASTWLQLLRYDRSSSDRPDILPAEAPPVASTPIVLAMPDPMARALGWPDAKL
                     GWADLLRLANDPAGWGAKGHPEWGAFKLGKTNPNISTTGLSATVGVFVAATGTSSDLT
                     LETLKDPHVREFVAGVERSVAHYGDTALTFLTNLQRADDAGAALGYVGAVAVEEKSVV
                     DYNNGNPTGDLQSDGRRAQPRVPLVAVYPREGTLNSDNPFAVLQAPWSEPGKKAGASD
                     FLAFLREPAQQRMFTGAGFRSYEGHPGGAVTDSVYLREDIGVPLLTPPSPPVLSAVRA
                     TWAELRKKAHLLLVLDVSGSMGLQAGAGKTRLELAQAAALHGLAQLSDADEVGLWVFP
                     SGGQVYWQYLAAEPVGPQRDEMNRRIQQLVPSGGTPLYAVTRKAVEEVGRTARTDTIN
                     AVVVMTDGMNENPDDNDLDGLIRQLTDQAPEGGVRVFTIAYGQDADVNTLKRISEASR
                     AAAYDATDPLTIDTVFTNVLSNF"
     gene            complement(21757..22680)
                     /locus_tag="VGP27_08835"
     CDS             complement(21757..22680)
                     /locus_tag="VGP27_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV7851453.1"
                     /translation="MGRRAFLAAAMAAFVAGCIRSDPGGRMRLAAGDPGGMYLAFAEI
                     LAKRVQARYPGVTVEVVATEGTVENLARLRAGDVDMGLALADIAERDRAADPSATAPQ
                     AVARVYENYLQVIVRSADVAQQLSDLAARRVSFGAARSGGAATSEVLFEAAGLRSRMD
                     VQTYRLKDALSRLADGGIDALVWSGGVPTPAIADLNGALPLRMLDIGALAEPMSRLAG
                     FPYVVRQVPSGGYVPPGIHSIGVPDLLLCRQHMATDLVGAVVDVLATDASQLVPPYVR
                     GLQYLAPPTMIQTGLIPLHPGAVDAYRELHG"
     gene            complement(22701..>22958)
                     /locus_tag="VGP27_08840"
     CDS             complement(22701..>22958)
                     /locus_tag="VGP27_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7851454.1"
                     /translation="TVATEKSDQGVELVISDDGTGLPDEDLAQASTRFWRAHNDLAGT
                     GLGLAIAAEITAGHGGTISIERAPEGGLLVRYGLPSSGESP"
BASE COUNT         3973 a         7913 c         7253 g         3819 t
ORIGIN      
        1 cccgtcggtg cgtgtcagga cttctgctga gacctgcttg ctcatgaact cggcgtcggc
       61 ggggtccacc acccgtgcga cctggtaagg cagtgactcc agcgagatcg gggcgctgag
      121 ttcggtggcc attcggtggg cggccacctc gaactgcatt gggccgaccg cagcgagcac
      181 tggcgcttga tccccgcgac gctctgagcg cagcacctga acgacgccct cctgctcgag
      241 ctgctcgatc ccgcggcgaa actgcttgtg cttactggga tctgtaccgc gcgccaccgc
      301 gaaatgctcg ggggagaagc tcggtatcgg cggatactgc acaggcacat cgcgatacag
      361 ggtgtcaccc ggacgcaggg ccgctgcgtt ggccaacccg atcacatcgc ccggccacgc
      421 ggtgtccagg gtggcgcgtt gctggccgaa taccgattgc gcgtacttgg tgacgaacgg
      481 cttgccggtg gcggcgtggg tgaggacatc gccgcgcgcg aatgtgccag tgcaaactcg
      541 cgcataggcg atccgatcgc ggtgggcgga atccatacca gcctgcacct tgaaaacgaa
      601 ggcgctgaaa ggggattcgg cagcgcgcca ggccccgtcg acgtcgactg ctccgctggg
      661 tggcggtgcg agttcgacaa gcacatcgag aagctgattc acgccgaagt tcaacgcggc
      721 cgaggtgaac aggaccggtg tggctgcgcc gttgaggtac tgctcacggt cgtaatcgga
      781 gccgtccgcg gacagcagtt cggactcctc gacggcgtga tcccagtcgt cgccggcggc
      841 actgtgggca tccggcgccg cgatgtgctc ttcgggcgcc gcggtggccc cgcccgcggt
      901 gcgggtgaac cggatgaagt tccccgtccg acggtcgagc acccctttga agtttccggc
      961 gatgccgacc ggccaggtta ggggagtggg gcgcagcccg atccgcgcat gaatctcgtc
     1021 catcagttcc agggcgtcgc gaccgggacg gtcccacttg ttgatcaccg tgatgatcgg
     1081 aatccgacgg tgtttgcaca cctggaacag cttcagtgtc tgcggctcaa gtcctttcgc
     1141 ggcgtcgatg agcatcaccg cggagtcgac ggcggtcagt acccggtagg tgtcttcgga
     1201 gaagtcggcg tggccgggag tgtcgagcag attgatgacg caggtgcggt atggaaactg
     1261 caacaccgtc gatgtgatcg agatgcccct ggccttctcc atctccatcc agtcagaaac
     1321 tgtcgcccga cgacccgcct ttccgtggac cgcgccggct tcggtgatga cccgggcgtg
     1381 aagcgccaga gcctcggtca acgtggattt gccagcgtcg ggatggctga tcacagcgaa
     1441 ggttctgcgg cggcccgcct cggtggagat gcggccacgt ggacagggat cggtagtcgg
     1501 cggagcggcc agggcgttgt cggtcacagc gtcagcgatg gtactggcta acgactgcaa
     1561 accagtaatt gacgaaggag agtagcatcc cgcgcccgac cgacaggaac gctgctccac
     1621 cgtcgattcg tcgaaccctt gctaacggtc ctcggcgtga tagagctgca gcgccagagc
     1681 acgcccattt tcggtgagct gtagccatgc gcaggccgtc cagccccagc ggtcgtcgaa
     1741 attggtcacg tatctctcct cgattttggc cattgcctcg tccagtgaca tgttccacgc
     1801 ctcgaagccg tcgttcgtat tcctgacaat ttcgcctaaa tcgaacaggc cgtcgtcaac
     1861 gagttcgcgg atcatgctca atgtcagctg ctgcgcctca gccaccggcc gtcttgaggt
     1921 gtagttgtcg aatagaaatg tgccatggat ctctccgaga cgaatccaat cccgtaaccc
     1981 ctcaatcagc agattttcct taacatcatc gtgctcggaa gttgccgtgc cgtcgtccac
     2041 cccatgaagc tacctccgtg agactggtcc cggatgcgcg aatttcgaga gacgcgccag
     2101 tcgcacaaat tggtgcaaag gcgactgttt ggcgctctta gactcggaat gtgggctgca
     2161 aacagtgttc accgaagtga gtctgacgct gtcgcaggtt cgtcaatggg acaccgcgca
     2221 cctgacgagc gctgcgcacc attggtccgc cacggccaat cggtgggacg acgccttcgc
     2281 ccagttggaa cggctgactc actcgccggg tggaacgcca tgggaggggg tggccgctcg
     2341 cgctgcccag gaccgatctc acgcggatcg acggcgcgtg agcgccatgg cagaccagct
     2401 gcacgccgca tcttctatcg cgagcagggc tgcaggagaa cttcggacag cgcagcaacg
     2461 cgttctcgcc attgtcgaca gggctgaagc gaggggtttc ggtgtgggtg aggacttttc
     2521 gctcacgaca taccgcgctg gtgcgcaaga gatcgcgacc gctgaagcag acatgcgtga
     2581 gttcggtcgc caattgcgcg accgcatcga agaattgctc gaactcgatc aacaagtcgc
     2641 cgaccgaatc gcggtagccg cgaacgatgt cggcgccctc acattcacag acagcgctga
     2701 ggcggatgta aatcacgacg gagcagttca ggctatcgat aaccgcgtcc tgaaagaggc
     2761 gccgcctcaa ccgcccccgc cggatccgac acctggaccc atgccaccta tcaacggcgc
     2821 tgatgatgtg agaagagttc tcgagcctct gcaaaacggc ggcaggaggg ggccaaacgg
     2881 cgtggggacc gagccggacg tcaaagagat atgggacgag tcctcgatga agcggctgtg
     2941 ggattacctc acacgcaatg ccaacgacgg tgtacccccg cccgactaca ggggtccggt
     3001 gcgggtgctc cccgatggca cgagaatcgg ttatcgcggg agcggtaggt ggggcgacac
     3061 cattgacgtc tggtatccag acgcctactt gaagggacac actccgtacg agccgtattt
     3121 tccgccgctc gtcagcggcc ctccgcaact ccctccggcc gctagcatcc cgtcggtgca
     3181 agtaatgccg ccgcagttga cacacccgcc gaccacgttg ccaccggcag ggattttcga
     3241 gccgaacgga ttgccgccgt ggttgcagaa tccgtctgca ccagggcttc acagtgcagc
     3301 tcaatccccg acgatcatgc cgggcgtagc cttgccagac acaccgcagg cgtcagcgcc
     3361 cgccccggac gacggtccgg ggctgtccga aatgggcgga acattcgtcg acacgggtga
     3421 ggcggtcggc agtctcatcg tcggcggcat cgtgatcgtc ggcggcctgc tggggacggt
     3481 agccacgcca agcggccaga tcgcccgctg actgcgcgct gtcgcccatc aaactccatt
     3541 acggatcgtc ggcgtgatac agccgcagcg caagagcctt ccctttttcc gtgagctgca
     3601 gccaaacgta aaacgaccaa ttccagcgct cgtcgaagtt ggtcacgtac gcatgctcga
     3661 tcttggccat cgcctcgtcc aatggcagat tccacgcctc gaatccggag tgcccttttc
     3721 tgactatttc gcccagatcg aacagaccct cctcggcgag ttcgcggatc atgttcaacg
     3781 tcagctgttg cgcctcggcc accggacgtt tgggagtctg attgtcgaaa gcgaactcgg
     3841 tatggatctc ggttagatgg atccagtcct gtaacccctc gatcagcagg cgttccttca
     3901 cgtcctcgtg ctcggaagtt gccttgtcgt cgtccacccc atgaagctac cctcgtgaga
     3961 cctgtttcgg atacgcggat ttcgagggac gcgcgcgtgg ccgatattgg cgcagagcca
     4021 actgttcggg gacccgctca cggctcgtcg gcgtgataca gcttcagcgc caacgccttt
     4081 cccctttcgg tgagctccaa ccaagcgcac gtcacccagt tccaccgatc gtcgaaattc
     4141 ttcacgtact tgtgctggat ttcggccatc gcttcgtcca gagacacgcc ccacgattcg
     4201 aatcctgatg gcctcttgtt gtctggctca cccagaacga acaggccctc gttgacgagt
     4261 tctcggatca tgttcaacgt cagctgttgc gcctcagcga ctgggcgttt gggcgcgtga
     4321 ttttcgaatg caaaggcctg gtgtatctcc gacagatgaa tccagtcctg tagtccctcg
     4381 atcagcaggc gttccttcac gtcatcgtgt tcggaagcgc cgacgtcgtc gtccatccgt
     4441 caaatctatc gccgaaacgc gccactccat tcagaagttc gcggtgaccg tcagcccacg
     4501 tcgtgcacgg cgatcagatg gtgtgacacc ggctcgtggt cgagaaactg ttcgaccgcc
     4561 tgctcgatca ataggtcgtt accggtgagc ctggtgtagt gctggtgcag atgctcaccc
     4621 cacgatcgca cgatgaaagt ctccacgaag gtgtgttcgc gctcgacgct gcggtagagc
     4681 cgccaccgcg ccgcgccggt gcgttgccgg gagcggccca tcgcggcgat ggccgtcagg
     4741 aacgccgcct cgtccttcgg ctcgacgcga taggtcgtca taaccaaaac cgggccgtcg
     4801 agaggttcgg gttcgaacac cagcgtcggt tccggccagt gcgacgacgg cgtcaggtcc
     4861 aggttgccgg tgcgggcgtg cagcggccac cacagcgtcg acaaggcgca caccgccagc
     4921 agccccgcac tgaccagcag gctcacgacg ctcgacgtcg cgccggcgag cacaccccac
     4981 accagcgaac ccagcgcttg accgcccatg aagatcagct ggtacaccga caatccgcgg
     5041 gcgcgcaccc accccggcag gctcagctgc atcgacgcgt tgagagtcga caggtacaag
     5101 agccacgccg cgccaccgaa cgccaatccg acgagcaccg ccacgaagtt atgcaccagc
     5161 gccagcacca cggtcgacgc ggcgaagccc gccgccgcga cggtgagcaa cgcgttctgg
     5221 ccgaagcgcg accgcagccg ggacagcacg agcgcaccga gtaccgcgcc gactcccagc
     5281 gcccccagca gcagcccgta gccggacgat gacaatccca gttgatcctt cgcgatcacc
     5341 ggcagcagac cccacagcgc actccccgga gcgatgaaca gcgcggtgcg cagcaggatg
     5401 cgccgcacga tcggggagcg gcggatgtag cgaccgccgg cactcagcgc cgccagcgcc
     5461 cgctccgccg ggaatctgtg ctcgatcgcc ggtttccgcc agacgacgag cacgatcacg
     5521 atgcccgcga acgacaccgc gttgagggcg aagaccaccg tcggccccgc cagcgacacc
     5581 agggcaccgg cgattgcggg gccgatcgca cgcgcaccgt tcacgctcat gctgcccagt
     5641 gctgctgcag cggggatctg ttcaggtgaa acgagatccg gttggattgc ctgccatgcc
     5701 ggtacggtga gcgcctgccc gcaaccgatg aggaacagca gcaacagcag caccgtcggg
     5761 gtggtcagtc cggcgcccgt cagcgacgcc agcagtgcga cccccgcggc catcgccgcc
     5821 tgggtcgcga tgagaagacg gcgtcggtcg accagatcgg ccaggacccc cgacggcaac
     5881 gccagcagca tcaccggcag cgtcgtcgcg gtctgcacca acggcaccag aaccgcggcg
     5941 cgcgggtcgc cgacgagcat ccattgcgca gccaccgtct gcatccaggt gcccaggttg
     6001 gagacgaact gcgcgatcca cagcgcccgg aacaccggcg accgcagcgg ggcccatgtc
     6061 gagcccgcgt ctaccggtgt cgctgaagtc atcgacagtc agtcttgcgc accgcgctgc
     6121 ctcgcgagaa tgcagccatg gtcgttgcga cgcgccatcc acgaccatgg ctgctatctg
     6181 acgcgttaaa acaacgctcg ctgccgagat cggcgcgacc gccccggcgt aagcgccggc
     6241 tgctcggcca ccgcacgccg actgcggcgc gcagccggtc ggcgcgcgct cagcttcggg
     6301 cagggtgcgc ctcatagaac tcgatgtgcc tccgtgcggc cgcctcccac gtcatcgaca
     6361 gcgccaattc ccgcccagcc gaagcgcggc tgtcgtcgcc caatgacgtg gccatcgcgg
     6421 cggcgaaccc aagaggatcg ttcgcgtagc gcaccgcctc gccgaacacc tcgcgcagca
     6481 ccggcaggtt ccgcgtaacg accggccgcc ccgccgccaa cgcctccatc gccgccagac
     6541 cgaaaccctc cttcgtcgac ggaaacccga acacctcact tgccgccacc aggctcggca
     6601 actctgtgtc ctcgaccgcg cctaagatga tcggcgcaac gtcgagctcg gaacacctcc
     6661 gctcgaactc cgcccggtag tcgcggtagt cgaacagcgt ttccccgccg gcgatcacca
     6721 gtgagatgtc gggaaaccgt tgccggaaca ggtgatacga cgacaccagg tccagtgatc
     6781 ctttgcgtgg ttcgataccg cccacagcca acacgtaccg gcccagctcg tcgcgccagc
     6841 gctgcttgcc gacaccatcc gacgcggccg cgatgaaccg gtccgcccgc acgccgttcg
     6901 gaatgaccgt cggatgcagc ccccaccccg cgacgacctc cgcggcgacg gccgccgaca
     6961 cacatatccg cgcgtacggt tccaccaccg ccttctcatg gcaggcgacg aggattggtg
     7021 tggtgaactc gtccaggtgg tgaattgtcc ggatgcacgg gcccacggcg ttggcgctga
     7081 tgcagtcctg cgcgtgcacg atgtcgtaat cgtccgccac gaagtcgccc cgcagcgtct
     7141 cgatcgaccg cacgatccgc tcggttaccg gttcgttctc gacctcagga aagtccacca
     7201 accgcaccgc tacccgcgga tccacggcac ggaagaagcc cgtgtcgccc tggcgggcca
     7261 gcgaccacac ggacacgtcg atcccgaagt ccgccagcgc ctcggccagg ttcagcgtgt
     7321 gcaccacccc gccgcgcggc ttggtcgagt atgtgatgag cgcgacgcgc accgctcaac
     7381 tcctcgaccg cgcccgatag gcatcgagcc tgcgctccgc cagatggttg agaacctttc
     7441 gcgccctgct aatctcactc tcgacgtcga cctcgacctt cgccagcccg cccttcgacc
     7501 gcgtcgtggc caggatgtcg ccgcccgggc ccaccacctt ggcctgcccc aggaactgca
     7561 gagcgcccat gaccccggtc tggttcgagg acaccaacac cacctggttc tccgccgccc
     7621 gcgcacagtc gtaaaggtcg aacagccgcg actgtcgatc ggcaggcagc cgcgacgccc
     7681 ggtcggtcac actggccggc cacgctgaca gcgccgcgat gatcgatgcc ccgtccagcg
     7741 ccagcgcccg agccgattcg gggaacgtct tgtcgtagtc gatcagcatg ccgatccggc
     7801 caaccggagt gtcgaacgcc gagaacgagt caccggcgag gtacgccaac gcctcgcccg
     7861 ccggttggtg caccttccga tgcgtgccca acaccccgtc ccccgacacg cagattgccg
     7921 agttgtaccg gccgccaccc gccgccgcct cggcgtagcc cacgcacacc gtcatcggcc
     7981 ccgccccggc tatcaccgcc gcgatctccg gtccgtccgg ggccaacgcc ggtggaggat
     8041 cgtcggggtc gggcgcacgc aggtcgccga tgtagccgcc caggcacgcg tcgggaaaca
     8101 ccaacagctg cacgccttct cgggccgctg cctcgacgat gacaacgacc ttttccacgc
     8161 accgggccac gtcccggccg aaattcgccg ccaccgcggc caccgtcacc ggcgccatgc
     8221 catccccgtc acgcccgacc caagccggtc aaccccgcgg cgaccgccgt cgtcacggcg
     8281 ccgtccggcc accggagccg cacaccgtgt tccacggtca ggcgtccgca tacctcgctg
     8341 gccacgccgg ccggcaccgg caccggggaa ccgtcgacgg tgagcatccc gaaaccgggg
     8401 aagcacgtca gccacgatcc catctcggca cctgcggggc gtggtatcgc cgaaacgtcc
     8461 agttcggcgc ccgtgccact ggcttcggcc atcatcgcgg ccgtgccgac gatccccgcc
     8521 atgctgacgt ccttcgccgc gcgtgcccgc atcgcgtcta ccaggccggc catcgtgacc
     8581 aggtcgtccg aggagcgggt gctcgtcgaa tcccattgtc gtccctgata acccggccgc
     8641 cagtgcccgg aaagatcggc ggtcagccgc aggttgtcgc ccgcgatgcc gccgccggcc
     8701 ggtaccggct gcgcggtgcg gcccaacgcc gtcaccgcca gcgctgccgg tacaccaagc
     8761 tgtgtatggc cccctagcac cggtacccgc cacgcctgcg cgccccgcgc gaccccccgc
     8821 atgatgcggg tcagcaggga ctgcgtcggt gcgccgaccg cgtccagaag accgaccggc
     8881 gcggcgccca tcgcggtcaa gtcgttgacg ttgaccagga ccgaacacca ccccgcccac
     8941 tccggatcgc gctccaccat cgacgggatg atggcgtcac acgcggctac tatgtcgctt
     9001 cccggcagag gtacaccgtc gtccccgacg aaacccgtcg gtcccaaccc gccctcgacg
     9061 gcgcgcagcg ggtccagcac cgcacccagg aacgacttcg tcgcgtcggc ggcacgctga
     9121 atctggttga gcggccaccg cattcgcaca tggtggcggc ccgccacgtc gcagtcgccg
     9181 acgctgttcc agccgagccc gatgaacagc tcctggtacc gacgttgcac ggtcgcctcg
     9241 aaccgcagcg cccccgcgga ctcgacatgg gcgcaggcgg cacggatcag tgcggcgccg
     9301 acaccggatg tccgcaccga aggatcggcg accagtcggc tgccggtcca ccagccgatg
     9361 tcgggtgcgc agcgcggcgt cagccgaacc ccgcctagca ccgaaccgtc cggtgccgcg
     9421 gccaccaaga cgaccgtgcg cgggtcgtcg tcggcgtcgt cgcggtcgct accggcgaac
     9481 aacccctgct cgacgacgaa cgcctcgcgc cgcaaccacc ggtactcgtc gccctcgcga
     9541 ccttccgccg gatggatcag gaaccccggc tgcgggtgtg tcctgacacc ggccagaata
     9601 gacaactcgc ggtggcgaat cacctcgtgc gccaacgttt tcacccgccc aggttctgca
     9661 ggacgccaca tgcgccgcac gccgcgcagc cggcccgttg gtcggccccc gacattcccg
     9721 ccaaccgcag ctgggcggcg acctcccggg acactttctc gaccagtgcg gcgtccggcg
     9781 ctcgggcagc atcgacgtcg acggccagag tgcccgggtg cggccggaac ggtacgacga
     9841 acgggtacac gcccatggcg atcagctcgg ccgcccccgc gaccagctcg tcggggtcct
     9901 cgcccagccc cacgagcagg tacgtcgaca cctggttgcg gccgaacagc cgcaccgcct
     9961 ctcgccaggc ctcgcggtac tgcgtcagcg gcaccgttgc cttgcccggc atccaccgcc
    10021 gccgcacgtc gtcgtccaac gactcgacgt ggataccgat ggcgtccgcg cccgcgtccc
    10081 gcagctcggt gaggaccgaa agatcggcag gcggctcgca ctgcacctgg atcggcagcg
    10141 caggcaccgc ggccttcacc gccttcacac accgggccaa gtggcgggcg ccgcggtcgc
    10201 tgccggcgga ggtgcccgtc gtcatcacca tttgagcgac accgtcgagg cgcaccgcgg
    10261 cttcggcgac tgcggcgagc tcggccggcc gcttgacggc ggtggtggcg cccgcgcgca
    10321 gtgactcctc gatggaacag aaccggcaac gctggtcggg ggagtagcga atgcacgtct
    10381 ggacgactgt cgtcgccagc acgttgcggc cgtgcagcgt ggcgagctgc tcgtacggca
    10441 cgccgtcgtc ggtcacgaga tcatagaact tcggccgctc gatcacctgg acgtccagac
    10501 cggtatcctc accgtcgaag aggatctggg cgccatcgaa catgaacgga ctgtgagggt
    10561 ttcgcggaat tgccgcgttg aggccgtcga tcaccaagtg gccgtcgtcg ctcgggcctg
    10621 cgcctgcggt ccgactgacg ggcggtcggc cccggatgcc cagcagggcg agatcgatac
    10681 gggttgacac agacatgacc gtctcctcta ctggcttgcg gccagtctca gcggggattc
    10741 ataggtggct gcaatcgtcg tggcgatatg ccgtgcgtcg tggtcgattc cgaggaacct
    10801 gcccgacccc caggtgtgca tccacggcag gccgacgaag ctcagcccgg gcacactagt
    10861 gacgccacgg ttctgcatcg gccgtccacc gccgtcgaac gcgctggcct cgatccagcg
    10921 gtagtccggc cggtagccga tcgcccacac gatgctggtg acgccctccg cagccagatc
    10981 taaagtggtg ggctctgttt cgggctgcca tgcccgcgtg tagcgcctcg gtggttcggc
    11041 tgagatgcgc tcccgttcga tgtaggagtc gatgtcggcg cagatcgagt tgtacacgga
    11101 atcggcgtgg tcgagcgacg cgcgcagcgt cggcgcgaac tgcaggctgc tgtgcttgcc
    11161 gtcggtcagt gccccgtaga ggcgcatgcc ctcgacggcg aagtggcgca ggtcgatatc
    11221 gcggccgccg tcgcggccgg tgacgtagtg attggtcttc tcgatggcgg ccttgccgcc
    11281 ggggtactcg cccgccgccc tgtcgtagag ccccatatcg gacagccacg tcatgcagtc
    11341 gcggccccgg tagaaccggg cgacgcgcgg ggcgctgccg accgcaagat gcacctgacg
    11401 tccttccagg tgcaggtcct cggcgatctg tgcgcccgac tgcccggtgc cgaccaccag
    11461 cacggcgccc tggggcagct gaccggcgtt gcggtactgc tcggaatgga tctgcgtcac
    11521 cgctgggtct agcgatccgg cgaacgcggg cgtcaccggc agtgggtaac cgcccgtcgc
    11581 gacgaccgcg tggtcgcagg tcatcgtttc ctcgccggcg tcggtgctca cggtgagctc
    11641 gaagccgccg ccgatgatgt tgcgcagccg ggtgactcgg gtgtgggcgc gcaccggtgc
    11701 gtcgaaggtg tccatccagc cggccagcca gtcgaccact tcgtctcggg tcatgaagcc
    11761 atctgggtcc ggacccgcgt aggagtatcc gggcagccgg cagtgccagt tgggggtgac
    11821 gagcgtgaaa ctgtcccacc ggctgtccgc ccaggcgtgc gcgggcgtct tggcctccag
    11881 cacgacgtgg tcgatcccgg ctcggctgag gtaccagctg accgacagcc ccgcctggcc
    11941 ggctccgatg atcgcaacag ggaggtgtgc cgtcatgacg tcgccaatgg aggttgcatg
    12001 gcggtgacgt gaacatgcgc cgagggctcg aatctgctcg ccacttcggc gatttcctcg
    12061 gtggtggctg ccgccgaggt gcacatgaag ccgaacttcg cgcgcacccg ctcgctggcc
    12121 tcggcgagcg ctatgcccga tcggtcgacg aaatcggcga cggtatagct ggcgcccgcg
    12181 ctgaggtagt cgtgcatgac gaggctgggg gagtagcagc tttggacgct gccgtcgggc
    12241 cagcggacat cgaatgtcat ttcaggcatg cgccagctcc cgccacttcg ccaccgcgcc
    12301 gtcggtcaac ggtccgtaga cgtcgggtct gcggtcgcgc agatggaaca ttccgccccg
    12361 catggcgcgg aacgtggagt cgatatccac ctccgcgacg gccatgccgc tgccgagaag
    12421 tgttgttgcc agcacatttc cgcctgggtc gacgaccttg gcgttgccga cgtagcgcag
    12481 tgagccgaac gtgccgctct ggttggacgc gatccagaac acctggttgt cgagggctcg
    12541 tgccgtatcg aacaggttga accggtaggt ccagcggtcg tcctgcaggt tttcggcggt
    12601 ggcggtgcgg gcggcgggcc acgcggacag gctggcgatg atctgtgcgc cctgcaacgc
    12661 catgatgcgg gcggcttccg gaaacgcctt gtcgtagcag atctgcagcc cgatccggcc
    12721 aaccggggtg tcgaacacgt cgtagcccga accggccgag tatgacgtgc cttcgccgag
    12781 gggttgatgg actttgcgat acgcgccgta gacggtgttt ccgtcgagca ccgctgcggc
    12841 gttgtagcgt gtctcgccgt cctcggcctg ttcgcagaag ccgatcgcga cgacgaggtc
    12901 gccgatgatc gcttgcacgt gggcgatctc cgggccgtca agccggatgg ccggcggcag
    12961 cgaaggggtg ctggtcttta ccgtgtcgcc gtggttgccc agcgacgaca ggtacccgcc
    13021 gatggccgcc tctggcaaca ccaggaagtc gaccgaattt tcccgcgcct cctcggccag
    13081 tgaggagatc agcgcgtagt tctgctccag gtcgcgggtg aagttggccg agaccgcggc
    13141 taatgtcgtc atctcacaat aactttcgac tggctgccga gatcagacca tgtaggtgga
    13201 gttgatgatc gcgcccttgc gtgcatagtg gatgaccgcg tcctgcacgt cgagcggatg
    13261 tgccgggatc acgccctcga tgagatcctc ttcgcgcccg ccatgcagtg ccaacccgaa
    13321 ccggcaacag aacaccgttc cgccctcgcc gatgaatgtg gccagcgagt cgttgatgtt
    13381 ctgctcgccg gggaagccgg agtcgccggt cttcggaaag ccgcgggtgg caaggcaatt
    13441 gatcgcaccc gggccgtaga agtagatcgc cgactcgaag cctttgcgca gtgcgcgggt
    13501 ggcctgcaac acggcgacga agctcaccga cgattcgtgg gcgataccgt gcacaagggt
    13561 gaaatacgtc tcgccgttct cggccttgta gtcggggaag atcttggtgc cgccgtagat
    13621 gttggagcct ttgggcagtg ccggatgtgg aatctccgca agcgaagtct tgatgttctc
    13681 gtcgatcgaa tcgtcaaaag gcatggcgat ctccatgttc agggtgagcg atgggcctca
    13741 cggcgtcgat gccgcgagcc gagtgaatcg ttcggctgca tcgattacag gcgccgagta
    13801 ttcacccttg gttagcgatg gattacaact gcgttaggag gaactcacag ccgaagcgcc
    13861 gggcgtaaca ctggaccgga gctggcgacc gccgagcacc gtgcacggcc cgacatctat
    13921 ctcctcccgg atcctgttcc gcctatgaac ttccgccccc atgttttctg tgcgaactga
    13981 gaaatggctg ggaaacgggc tgttttcacg cagaactgcg gctagcaggt aatcacacat
    14041 agcggctttc ccccacgcct ggttctcgct gcgtcttggt cactgatcgc cgggccatca
    14101 cttggatccg ttgccccgca acactcgtcg cccccgatta gtacgagcac caagcctgtc
    14161 ctggaagatg cgacttactc gggctgagtc gccaacagct tcggatgcaa aaccgtcacg
    14221 ccaggccttg acgacgtcct cgacacgctg gtgcacttgt gctcgagcgc cgcggagacc
    14281 ttcttcatgt cgtagatcga gcatcgggtc gttggtgttg cgggtcgact ctgtaagccg
    14341 cgccagcgcg gcaggcagtg aagcctcacc accgcgcagt cacattggcg gcgagctgtt
    14401 cggcaatctt gacggccgaa tcagcggggt ccgcgctgca cgtgttgacg tcgacaatga
    14461 tgttgtttct gtgcgtcagc gcgcgtccgc agccccagcc gggggcggcg gcgtcctgct
    14521 cggtcgcggt gacactcagt gcgccgtcag cgttggagat ctgtccgacg gaccactgcg
    14581 tcccgctctg cgtgtgggtg tacccgcggc atgccggcca ccgctcggcg gaggcgtcga
    14641 agaaggcgcc ggctttttcg atggtgggga acaacaccag cgcctgcttg agatagtgcg
    14701 tgaacttgtc gccgttgttg aggctctgat cgcgctcggc ccagtaaccg ctgtcggcat
    14761 agaccggtgc ctcggcggcg ccgtcgatgg ccaggcactc cggcggcgcc atggtggcgc
    14821 tgttgtcaga catcgaggcc tgctgatttg tgaccgccat cccggccgtc cccatagcga
    14881 agttcacctg ttcagggttg agcagaagcc ctaccagctc gcgctcgaca agagggcgcg
    14941 gaatcatcga ccgggtggtg gtgggcatct cggcgttgcc tatcgtgctg gcgcacccgg
    15001 cgatcgaaac gcagatgact gcaaccgcaa gtgcggacgt tggctggcgc atgtgttcct
    15061 ccccatgcca gttgtgccca acctggtcag cctggcgcga cctcccccgt cgcacggctc
    15121 ataagaaatg gtgcaacaat tccgccgacg cgtgcgttag ttgaccgatg ttttgggtaa
    15181 cgaagcaccg gcgtttttga taacgatcaa aggaggccgt cccgcggagc tgttccgtcg
    15241 cgttgaggtc acggcggtcg cagccgcatg aatggcggcc gcagccgtct gagcagggcc
    15301 ttcgtccgcc agatagtgtg tagcacaagt gctacctcgc ggggtttcaa tcgtggactg
    15361 agagagctac gtcagcacgc atccgagctc gtgcggcgcg cgaagcggtc accgcgccgg
    15421 attgaacatc ggcagccccc cagaagaagt gggtaggttg ctctcgtggg tgtcacgcca
    15481 gccgacctga ccggtaccga gcgcgccgtc ctatgcgtgt tgatggcgga gtcgcgtccc
    15541 ttgccgaacc cggacctgat caacctcggg ccgaagctcg acaaacccag ccgcgacaaa
    15601 ctcaacggcc tcggcctgat cgagtcgaac cgcgaaagcg gacggttcgt ccacgagctc
    15661 accgacaagg ggtggcgcct gtgccgcgag atcatcgccg cgggcccacc cccgcgatcc
    15721 accggtcccg cgaagacgct ctacaccgtg ctgggcgcgc tgggtcgcta cctcaacggc
    15781 gccgaactga gtctcgccga cgttttcggc agctccgatg gcgtgcacgc gacggcgtcg
    15841 atcgaggacc gcatcagatc ggcctatgcc gacctcgcgc cacggccagg cggctgggta
    15901 agcctcaccc gcctgcgcgc tgagctctcc gacgccgacc gcggcgacat cgacaccgcc
    15961 ctgcggacgc tgtaccgcgc ggccggagtg agtctgatcc ccgaggagaa ccagaaggtc
    16021 ctcaccactg aggatcggca cgccgccgtc ctgatcggcg atcagagcaa gcacctcatc
    16081 gcgatcgaaa tttgatgcac gaggctgaac gggaagcact cgcgtcgctt cggctgacgt
    16141 gggctcccac cacggacgac ctgtggcgac cccaggcagc gacacatgtc ccaggcctca
    16201 acgagtacgc cgtcgacgat gtgatggccg cgttcggcga cgccgcccgc gaccccgtct
    16261 cggccccact cggtgtcgtg atccgtggga cggccggtac ggggaagacg cacctgctcg
    16321 gacagatccg cgagcgcgtg caagctgcca acggcttctt cttcgtcgtc gaactgctcg
    16381 acgcggccag cttctgggac tccgtccgca gtggtgtgtt ggagagcctt ggccgacctg
    16441 gcgcgcagcg ggagacacaa ctgaaggacc tgctctggga gctgtcgtcg atcgcgcacg
    16501 tctcccgcgc cgagcgccgc gcgatcatcg gcgacgacga cctcgatcgc gagacgctcg
    16561 accgcttcgt caacgcgctt gccaaggctc accgcgatcc agcacgcgat taccaccaga
    16621 cgttgcgcgc actcgtgctg ctcgccgctg ccgacctgac cgcccacgac gtcggcgagg
    16681 cctatctgca aggcagcgac gagtccggcg atgacggccg gagccgatgg ggactacgcg
    16741 gatcgcctga ctcggcccag gagtgcgtca aaggggtcgc gcgcctgatc gccctcgccg
    16801 gaccggcggt gctcgcgctc gaccaaatcg acaccctgct ggcgcagtcg ctgtcccgga
    16861 ccgacgccac cgaccgcgac ggcggcgacg ccgtcctcca ccaagtcgcc gacgggctga
    16921 tgtccgtccg ccagacgatg cgccgcaccg tcggcgtggt ggcgtgcctg ccagctgttt
    16981 gggagggcat ccgccacggc gcgacggcga ccgtcgccga ccgcttccgc gagaccagcc
    17041 cgctgaaggg cctgccctca gccgacatcg gccgcgccat cctcgaacgg cgtttcgccg
    17101 caagctacgc ggaagttggt ttcagaccgc tctatccgag ctggccgatc cgaccggagg
    17161 ccttcgccga tgccgccgat cacacgccgc ggcacctgct gaagatcgcc gacgcccatg
    17221 tgcgccagtg cctcaagcac gacacggtcg tcgaactcga tcgtctcagc gcccactggg
    17281 atccggaacc cgtcgacccg attccgattg acccgaggcc ctcagaactc gatcgccgct
    17341 tctcggagta ccggcagaac gccgtgaccg ccgccgcgct ggacctcgaa ggcgaagaca
    17401 ccgctgtgcc ggaacttctt cgtgccgccc tcacggcatg gatcaccgaa cgcgacgaag
    17461 actctcagtc gtacacctgc gacccgccgc ccggcgctca cgtcgtgctg cacgcgcggc
    17521 tgcgccagag cctcgacccc acgaccgaag acgagcggca ctgggctttc cgggccatcg
    17581 ccgccagcaa tgcggtcgcc gcgcaatcca ggatcaagaa gtcctggagc gtaacgggac
    17641 tcggggaggg atcgaacagg cgaaaattgt tcctgttgcg caacaccccg tggcccacgg
    17701 ggccgaagac ggccgcactg gtagaggaat tccaccgtgc cggcggccgc accgtcgaac
    17761 tcaccgaaga cgacatccgc acgatgacgg ccctgcgcga tctgatcggc gaggacgatc
    17821 acgacctacc cggctggctg cgcgcccgcc gtccggcaca cggtctggca ctccttcagg
    17881 aggtactcgg cgaagacggc cgcgcgccgc ggccaactca gcggttacct cctgccccgc
    17941 aaccggttcc caaacgcgag cccgaaccgg caccggcgcc caccgtcgtc gacgacactc
    18001 ctgactacgt gccggacgat gaactgccac tgggtgccga tgtccggtcg gatgcgacgg
    18061 tgtcggtcga gctgtcggcg ttgcgcaagc acatcgcgat cttcgccgga tccggctcgg
    18121 gcaagacggt gctgattcgt cggcttgtgg aggagtgcgc gcttcgcggt gtgtcgtcaa
    18181 tcgtgctgga ccccaacaac gatctcgcgc gacttggcac cgaatggccc cagcatccga
    18241 acgggtggcg acggtctgac gacagccgag cgtcggagta cctgaacaac accgaggtcg
    18301 tcatctggac gccccatcgc aacggtggac ggccgctggt ctttcagccg ctgccggact
    18361 ttccgagcgt cgtcgatgac gccgacgagt tcaacgacgc tgtggagtcg gcggcggccg
    18421 cgctcgaacc gcgtgcgtcg attgcagggc agtcgcagaa ggcgcaccgt tcccgcgctg
    18481 tccttcggga agcgttgcag tggtatggaa gacagccgac gccgacgctc ggcgggttcg
    18541 tcgacatact cgacgatccg cctgacgacc tgagcggctt ggctgacgcc cgcaagattg
    18601 cctcggacct ttcgcagaat ttgcgtgcgt cgatggtcaa tgatccgctg ttcggcggca
    18661 gggggacgcc ggttgaccca gcagtgctcc tcacaccgtc ggacggccac cgggcgcggg
    18721 tgtcggtcat cagcatgatc gggttgtcgt ccgacgaaca gcggcaagga ttcgtcaatc
    18781 agttgcagat ggcgttgttc gcctggatca agcgcaatcc cgcgggggac aggccgctcg
    18841 gtggccttct ggtgatggac gaggcgcaga cgtttgtccc gcggcagggc ttcgcggcat
    18901 gtcgccgaag cacattggcg ttgtcgtcgc aggcgcgcaa gtacgggctc ggactggtgt
    18961 tcgcaaccca ggcgccgaag tcgctggaca accagatccc cggcaacgcc gcgacgcagt
    19021 tctatgggtt gctcaacgcg ccggcgcaga tcgagaccgc taaagaaatg gctcgagcga
    19081 aaggtggtct ggtgccggac atcagcaggc tgcgggccgg caacttctac gtcgccaccg
    19141 agggtcaggc gttccaccgg atcgtcgcgc cgtggtgcct gacgtaccac ccgccgagcc
    19201 ccctctcgcc ggaggaagtg ctcgggctgg cgcgaggcga ctagcgacgt cacgccgcgt
    19261 cggcctccag ttcggcgaag gtcatttgca ggagcgtcac cgagtcgttc actgcgccga
    19321 catctccgtc gtccagtgcg acgcccatgg tgcgcgctgt cgccgacagc tcgagcagcc
    19381 ctgcatagac ggtggccacc tcatccaccg ccgcggtgag cttttcgacg agcccgtccc
    19441 gccgcgactg caaccgacca accgtcctgc cgatcgaatc caccgtgtgc tcgcctcgtc
    19501 cggaaacatt gcaggccaat gcaatcgcct cgtccatcgc atccaccgct gccgccgtct
    19561 gcgccaccgt cggccgcacc gcatcggcag ccgccaatgc cttgtcggcg tgaaaacgca
    19621 caacctccgg taacgcctga ctctgcagcg cccgcagcga ccgcacgtga ccgtcgaaaa
    19681 gcgtgatcag cgcatactgc tgcgtgccgg tctgcaacgt ccgccgccaa gacacaccgc
    19741 cgaccgtaac cgccgtaccc cacatcacta ccgccgtcac cagtgggaag gggaaatcca
    19801 acgcccacgc catcgtcccg ccgaacgctg acagcaacag tacccacggg tccagcgccc
    19861 gtctgccgaa actcgtggcg atcatcagaa gttcgacaga acgttggtga acaccgtgtc
    19921 gattgtcaac gggtccgtgg cgtcatatgc cgctgcccgc gacgcctccg agatccgttt
    19981 gagcgtattc acatcggcgt cctgcccgta cgcgatggtg aagactcgga caccgccctc
    20041 aggagcctgg tcggtcagct gacgaatcag cccgtccaaa tcgttgtcgt cagggttctc
    20101 gttcatgcca tcggtcatca cgacgacagc gttgatcgtg tccgtacgcg cagtcctgcc
    20161 cacctcttcg accgccttcc ttgtgacggc atatagcggt gtaccacccg acgggaccag
    20221 ctgctggatc cggcggttca tctcgtctcg ttgcgggccg accggttccg cggcgaggta
    20281 ttgccaatac acctggcccc cgctcgggaa gacccacaac ccgacctcgt cggcgtcgga
    20341 cagctgcgcc aggccatgca gcgccgccgc ctgcgccagc tccaaccttg ttttgcccgc
    20401 gcccgcctgc agccccatcg atcccgagac gtccagcacc agcagtaggt gcgccttctt
    20461 gcgcagttcg gcccaggtcg cgcggaccgc ggacagcacc ggtgggctgg gtggcgtcag
    20521 tagcggcact ccgatgtcct cgcgtaggta cacactgtcg gtgactgctc caccgggatg
    20581 accttcgtaa gaacggaatc cggcccctgt gaacatcctc tgttgggcgg gctcacgcag
    20641 gaacgcaagg aaatcagagg cgccggcttt cttgccgggc tccgaccacg gcgcctgcag
    20701 aaccgcaaac gggttgtcgc tgttgagcgt tccctctcgg ggatagacgg caaccagcgg
    20761 cacccgcggc tgagcgcggc gaccgtccga ctgaagatcc cccgtggggt ttccgttgtt
    20821 gtagtccacc accgacttct cctcgaccgc caccgcgccg acgtagccca gcgccgcgcc
    20881 ggcgtcgtcc gccctctgca ggttcgtcag aaacgtcagc gccgtgtcgc cgtagtgtgc
    20941 gaccgacctc tccacaccgg ctacgaactc gcggacgtgc ggatccttca gcgtctccag
    21001 cgtcagatcg ctagacgtgc ccgtcgcggc aacgaacact ccgaccgtcg ccgacagacc
    21061 cgtcgtggaa atgttcgggt tggtcttgcc cagcttgaag gctccccact ccgggtgacc
    21121 cttggcgccc catcctgctg ggtcgttggc aagacgcagc agatccgccc agcccaactt
    21181 cgcatccggc caccccaacg cacgcgccat cgggtcgggc atcgccagca cgatcggcgt
    21241 cgacgccacc ggcggcgcct ccgccggaag gatgtccggc cgatcagacg aagagcggtc
    21301 gtagcgcagc agttgcagcc acgtcgacgc cgccggcgac cacgcatccg gggccggacc
    21361 ccaggtcggc tcccaacgac cctccgcaag ccgcgactct gcgagtccgg aagtcattga
    21421 agcgatggct attccgtagc aatggccatc ggccagccgg gaggaggtgt tgtagcgctt
    21481 ggccgcctca tccatcaagt cggacttctc caccgatgcg gccaccacca cggtggtgca
    21541 tcccgcccgt gggccggccg cgttgtcgtc gtgttgcttc caccagtagg cgacgccgac
    21601 gaccatcaac cccgcgactg ccgcagccag caatgccctt ccgttcgtgg ccattttgtg
    21661 cctcccttgg tgttgttgca ctatggtggg cgcgtgcgct gctacgggaa aggcggtccc
    21721 gccacaaact cgaatttcct tgcacaatcc tcagattcag ccgtgcagtt cgcgatacgc
    21781 gtcaacagcg ccggggtgca acggtatgag gccagtctgg atcatcgtag gcggtgcgag
    21841 gtactgcaga cctcgcacat aaggaggtac taattgcgaa gcgtctgtgg cgagtacgtc
    21901 caccaccgcg ccgacaaggt cggtggccat gtgctggcgg cacagcagca gatcgggtac
    21961 cccaatggaa tgtatgccgg gtggcacgta cccgccggac ggtacttggc gcacgacgta
    22021 ggggaagccg gcgagccggc tcatcggttc agccagcgct ccgatgtcga gcatccgcag
    22081 cggcagtgcg ccgttgaggt cggcgatcgc cggggtcggc acgccgcccg accagaccag
    22141 tgcgtcgatg ccgccgtcgg ccagccgtga gagtgcatcc ttcaatcggt acgtctgcac
    22201 gtccattcga ctccggaggc cggccgcctc gaaaagcacc tcgctggtag ccgcaccacc
    22261 agaccgggct gccccgaacg acacccgtct tgccgcaaga tctgaaagct gttgggcgac
    22321 gtcggcggaa cgcacgatca cctgcagata gttctcatac acccgggcca ccgcctgagg
    22381 cgcggtggcc gaaggatcgg cggctcggtc ccgctcggcg atgtcggcga gtgccagccc
    22441 catgtcgacg tccccggcgc ggagacgggc caggttctct acagtgcctt cggtcgcgac
    22501 gacctcgacg gtgacacctg ggtagcgtgc ctgaacccgt ttcgcgagaa tctctgcgaa
    22561 cgccaggtac atgccgcccg gatctcctgc ggccaaccgc atgcgcccgc cggggtcaga
    22621 ccggatacag ccggcaacaa acgccgccat cgctgctgcc aagaaggcac gccggcccac
    22681 tacgccggac ccgttagtaa tcaaggtgat tcacccgacg acggcaaacc ataacggacg
    22741 agtagacccc cttcaggggc ccgctcgatc gagatggtcc ccccgtgtcc ggcggtgatc
    22801 tcggcggcga tcgccaaccc aagacccgtg ccggcgaggt cgttgtgcgc acgccagaac
    22861 cgggtcgatg cctgagccag gtcctcgtcg ggcaggccgg taccgtcgtc gctgatgacg
    22921 agttcgacgc cctggtcgct cttctcggtc gccaccgt
//
LOCUS       DASYLX010000360         7082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2977915,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000360 DASYLX010000000
VERSION     DASYLX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977915"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VGP27_08845"
     CDS             complement(<1..468)
                     /locus_tag="VGP27_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7851455.1"
                     /translation="MTIVLLSELRVLDLCNGDADAVTRSYADLGADVLKVEPPGGCPG
                     RSQPPCVAGAGVPFWLNNANKRSCVLDPADATDRDRLVELAAAADIVVDSGNPGRADA
                     FGTSCAALSRRYPHLVAMLVTDFGADGPRASWSATDPVLYALSTALSRSGPTTG"
     gene            537..2966
                     /locus_tag="VGP27_08850"
     CDS             537..2966
                     /locus_tag="VGP27_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEV7851456.1"
                     /translation="MSTDLHTTTAPRLSDDELSGIDAYWRAANYLSVGQIYLLDNPLL
                     RDRLAAEHIKPRLLGHWGTTPGLNLLYAHLNRIIRERDADVIYITGPGHGGPGLVANA
                     YLEGTYSEVYSGIEEDLDGMRKLFRQFSFPGGVPSHVAAETPGSIHEGGELGYALVHA
                     YGAAFDNPDLVVACVIGDGEAETGPLAASWHSNKFLNPATDGAVLPILHLNGYKIANP
                     TVLARIPHEELESLLTGYGYRPITVAGDDPANVHQQLAVALDSAFDQITAIQQAARAD
                     GVIQRPRWPMIVLRTPKGWTGPHEVDGKQVEGTWRSHQVPLSETRTNKKHLAQLEQWL
                     RSYRPEELFDDEGVFRPALRALAPAGARRMSANPHANGGVLLRDLDLPDFRDYAVAVD
                     KPASEAAEATKVLGTFLRDVITRNLDRFRLMGPDETASNRLSAVFETTDKVWLADTEP
                     GDENLSPDGRVMEVLSEHLCQGWLEGYLLTGRHGLFNCYEAFVHIVDSMLNQHAKWLS
                     SSSDLSWRRPIASLNYLLTSHVWRQDHNGASHQDPGFIDHVANKRPEVVRVYLPPDAN
                     TLLSVADHCLRSRHYVNVIVAGKQPALTYLDMDQAIAHCTRGIGIWEWASNAAGEPDV
                     VLACAGDIPTLEAVAATDILRRELPRLAVRVINVVDIMRLQPDTEHPHGLSDREYDAL
                     FTTDRPVIFAYHGYPWLIHRLTYQRANHAQMHVRGFKERGTTTTPFDMVMLNDLDRFH
                     LVIDVIDRADGLATRAAGLRQRMADARLAARRYTRENGEDDPAISGWTWDSGFRPSGT
                     QSMAQDTADNE"
     gene            2996..3751
                     /locus_tag="VGP27_08855"
     CDS             2996..3751
                     /locus_tag="VGP27_08855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV7851457.1"
                     /translation="MSDQAVSPHPAVAQLSVLHHFRTYIDIAVVVVMLAITNLVAHFT
                     TPWASIATVPAAAVGLLILVRSRGLGWAELGLGREHWRSGAGYALAAVAVVLTVIAIG
                     ALLPWTRPMFMNNNYATVSGALIASMIIIPLQTVIPEELAFRGVLHGALNRAWGFRGV
                     AAAGSLLFGLWHIATSLGLTSSNVGFTRIFGGGVLGTAAGVVLAVAATGVAGFVFTWL
                     RRRSGSLLAPIALHWSLNGMGALAAALVWHLST"
     gene            complement(3743..4330)
                     /locus_tag="VGP27_08860"
     CDS             complement(3743..4330)
                     /locus_tag="VGP27_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV7851458.1"
                     /translation="MPASHWLGDDESKPAPEPLSRIQALVNTIEMPEGQDRLADSAEA
                     RSWLVAHGLLRPDSECRADDLALVRGVREGLRAMLVRNAGGPPPTAAVLAPLREVAGD
                     GTASAVLDEKGEILLSATGDTVCGRLIELLLVIRDAQRDGTWARLKACGNNECQWAFY
                     DRSRNHGGTWCDMSSCGNKLKNREFRARKRGAPSR"
     gene            4449..4937
                     /locus_tag="VGP27_08865"
     CDS             4449..4937
                     /locus_tag="VGP27_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851459.1"
                     /translation="MSANRISISFLSAAQLQDARRREEMSRAPLNARLTARLFATRFD
                     RMLAVGVSAPAGSALAVHAERLTSVDEREALARSLRRSVGDARNRYARMSSRVPLNIP
                     NITAAQDRIDQVTLRLHSPRPVSARGIARLRVLLADGTGPMYRYGRGDLEGRLGAALA
                     AL"
     gene            complement(4950..5969)
                     /locus_tag="VGP27_08870"
     CDS             complement(4950..5969)
                     /locus_tag="VGP27_08870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV7851460.1"
                     /translation="MTSAKSMTAWQVRDPGPMSTRPLERVTVAVPSPGQGEILVAVRA
                     CGVCRTDLHVAEGDLPVHRPNVIPGHEVVAEVVAVGQDAGDEFTVGDRVGVAWLRHTC
                     GQCTFCIRGDENLCPQSRYTGWDADGGYAEFATAPAAFAHPLPIGYSDNELAPLLCAG
                     IIGYRSLLRADLPHGGRLGIYGFGGSAHITAQVALAQGAEVHVMTRGAVAQELALALG
                     AASAQGAYDPPPVKLDAAILFAPVGDLVLPALEALDRGGTLAIAGIYLSDIPALNYDR
                     HLFNERQVRSVTSNTRDDAREFLAFAGRHRIAVTTPEYPLDRAGDALADLSAGRIAGA
                     AVLRV"
     gene            complement(6043..6345)
                     /locus_tag="VGP27_08875"
     CDS             complement(6043..6345)
                     /locus_tag="VGP27_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7851461.1"
                     /translation="MDAITATELAARSTTLTSVGWIGYIIIGAIAGWIAGKIVKGGGS
                     GILMNIVIGIIGALIGGFLLSFFVDTAAGGWWFTLFTAILGSVILLWIVGMVQRKN"
     gene            complement(6483..>7082)
                     /locus_tag="VGP27_08880"
     CDS             complement(6483..>7082)
                     /locus_tag="VGP27_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0050840 - extracellular matrix binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APA family fibronectin-binding glycoprotein"
                     /protein_id="HEV7851462.1"
                     /translation="PAPATQPGQQGAQPANDTSVLLGRLDMKLFAGAEADNGKAATRL
                     ASDMGEFFMPFPGTRVNQESGALAAGDMPGAFSAYDVKFTDTNKPTGQIWAGVVGNAS
                     PTVAPGQSLAPRGQRNERWFVVWLGTANNPVDKGAAAALANSIRPWSPPPPPEPDPNA
                     PAPADPNAPPAPNNAPGRPPVGVGVPVDPANAPGMMPAG"
BASE COUNT         1198 a         2429 c         2322 g         1133 t
ORIGIN      
        1 ccctgtcgtc ggccctgacc gggacagcgc cgtcgacagc gcatagagca ccgggtcggt
       61 ggccgaccag gacgcccgtg gaccgtcggc gccgaagtcg gtgaccaaca tcgcaaccag
      121 gtgcggatac cgtcgcgaca gcgccgcaca cgaggtgccg aacgcgtcgg cgcgcccggg
      181 gttaccgctg tccaccacga tgtcggccgc cgcggcgagc tcgacaagac gatcgcgatc
      241 cgtcgcgtcg gcggggtcca gtacacagct gcgcttgttg gcgttgttca gccagaacgg
      301 aacaccggcg ccggccacgc acggcggctg cgaccgtccc gggcagccgc cgggcggttc
      361 gaccttcagc acatcggcac cgaggtcggc atacgaccgg gttaccgcgt cggcgtcgcc
      421 gttgcacagg tccagcaccc gcagttccga cagcagcacg atggtcaggt taatgcttag
      481 gtcttcgtga gccgggtacc actcgttgtt cacccaccac gacaaccatg gttccgatga
      541 gcactgacct ccacacaacg accgccccgc gcctttccga cgacgaactg tccgggatcg
      601 acgcctactg gcgggccgcc aactaccttt ccgtcggcca gatttatctt ctggacaatc
      661 cgctgctgcg ggaccggctg gcagccgagc acatcaaacc gcgactgctc ggccactggg
      721 gcacgacacc cgggctgaac ctcctctacg cccacctcaa ccgcatcatc cgcgagcgcg
      781 acgccgacgt catctacatc accggccccg gccacggcgg ccccggtctg gtcgccaacg
      841 cctacctcga gggcacctac agcgaggtgt attcgggcat cgaagaggac ctcgacggga
      901 tgcgaaagct gttccggcag ttctccttcc cgggaggcgt ccccagccac gtggccgccg
      961 agacgccggg atcgatacac gagggcggcg agctgggcta cgcgctcgtc catgcctacg
     1021 gcgccgcctt cgacaacccc gacctggtgg tggcctgtgt gatcggcgac ggcgaagccg
     1081 agacgggtcc gctggcggct agttggcact cgaacaagtt cctcaacccg gccaccgacg
     1141 gcgcggtgct gccgatcctg cacctcaacg gctacaagat cgccaacccc acggtgctcg
     1201 cccggattcc gcacgaggaa ctcgaatcgc tgttgaccgg ctacggttac cggccgatca
     1261 cggtggcagg cgacgacccc gccaacgtcc atcagcagct tgccgtcgcg ctggactcgg
     1321 ccttcgacca gatcaccgcc atccagcagg cggcgcgcgc cgacggagtc atacagcgtc
     1381 cgaggtggcc gatgatcgtg ctgcgcacgc ccaaggggtg gaccggtcca cacgaggtgg
     1441 acggtaagca ggtcgagggc acttggaggt cgcaccaggt cccgctgagc gagacgagga
     1501 ccaacaagaa gcatctggca caactcgagc agtggctgcg cagctaccgg ccggaggagt
     1561 tgttcgacga cgaaggcgtg tttcggcccg cgcttcgcgc cctggcgccc gcaggcgccc
     1621 gtcggatgag cgccaacccg cacgccaacg gcggcgtgct gctgcgcgac ctcgacctgc
     1681 cggacttccg tgactacgcc gtcgcggtgg acaagccggc gagcgaagcc gcagaagcca
     1741 ccaaggtgct cggcacgttc ctgcgcgacg tcatcacgcg caacctcgac cggttccgtc
     1801 tgatgggtcc cgacgagacc gcttcgaacc ggctgagcgc ggtgttcgag accaccgaca
     1861 aggtctggct ggccgacacc gaaccgggcg atgagaacct ctcccccgac ggccgggtga
     1921 tggaagtgct gtcggagcac ctgtgtcaag ggtggctcga gggctacctg ctgaccggca
     1981 ggcacgggct gttcaactgc tacgaggcgt tcgtgcacat cgtcgactcg atgctcaacc
     2041 agcacgccaa atggctgtcc agcagctccg acctcagctg gcgacgaccg atcgcatcgc
     2101 tgaactatct actgacatca catgtttggc gccaggatca caacggcgca tcgcatcagg
     2161 atcccggttt catcgaccat gtcgccaaca agcggcccga agtcgtgcgg gtctacctgc
     2221 cgccggatgc caacacactc ctgtcggtgg ccgaccactg cctgcgcagc cgtcattacg
     2281 tcaacgtcat cgtcgcgggc aagcagcccg cgctgacata cctggacatg gaccaggcga
     2341 tcgcgcattg cacccggggc atcggcatct gggagtgggc aagcaatgcc gcgggcgaac
     2401 cggatgtggt gcttgcctgc gcaggcgaca tcccgacgct ggaagccgtc gcggcgaccg
     2461 acatcctgcg ccgagaactg ccccggttgg cggtccgggt gatcaacgtc gtcgacatca
     2521 tgcgactgca acctgacacc gaacatccgc acggcctttc ggaccgcgaa tacgacgccc
     2581 tgttcaccac cgacagaccg gtgatctttg cctaccacgg ctatccctgg ctgatccaca
     2641 ggttgacgta tcaacgcgcc aaccacgctc agatgcacgt gcggggattc aaggagcgcg
     2701 gcaccaccac gacgccgttc gacatggtga tgctcaacga cctcgatcgc ttccatctcg
     2761 tcatcgacgt catcgaccgt gccgatggac tggccacccg cgccgcgggg ctgcgccagc
     2821 ggatggccga cgcacggctg gcggcgcgcc gctacacccg ggagaacggc gaggacgatc
     2881 cggccatctc gggctggacc tgggattcgg gctttcgtcc gtccggaaca cagtcgatgg
     2941 cgcaggacac cgccgacaac gagtgatgcc agcaggcgcc ggtacactcg gcagcgtgtc
     3001 cgaccaggcg gtgagcccgc atcccgcggt ggctcagctg tcggtgctac accatttccg
     3061 tacgtacatc gacattgcgg tggtcgtggt gatgctcgcg atcaccaacc tggtcgccca
     3121 cttcaccacg ccgtgggcca gtatcgccac ggtgcccgcg gccgccgtcg gactgctgat
     3181 cctggtgcgg tcgcgcggcc tgggctgggc ggagctgggc ctcggccgcg agcactggag
     3241 atcgggcgcc ggttacgcgc tggcggcggt cgccgtcgtg ctgacggtca tcgcgatcgg
     3301 ggcactgctg ccgtggaccc gcccgatgtt catgaacaac aactacgcca ctgtctccgg
     3361 cgcgctgatc gcttcgatga tcatcatccc gctgcagacg gtgattcccg aggagctggc
     3421 gtttcgcgga gtgctgcacg gcgcgctgaa ccgggcgtgg ggtttccgcg gcgtggcggc
     3481 ggcgggctcg ctgctcttcg gtctatggca catcgccacc tcgctaggcc tcaccagcag
     3541 caatgtcggc ttcacccgga tctttggcgg cggcgtgctc ggcaccgccg caggagtggt
     3601 gctcgccgtg gcggcgacag gtgtagccgg cttcgtgttc acctggcttc gccgcaggag
     3661 cggtagcctg ctcgcgccga tcgcactgca ctggtcgctc aacgggatgg gcgcacttgc
     3721 ggccgccctg gtgtggcacc tgtcaacgtg acggcgcgcc gcgcttgcgt gcgcggaatt
     3781 cgcggttctt gagcttgttg ccgcacgacg acatgtcgca ccaagtaccg ccatggttgc
     3841 gggagcggtc gtagaacgcc cactggcact cgttgttccc gcatgccttg agccgtgccc
     3901 aggtgccgtc gcgctgggcg tcgcggatga ccaacagcaa ctcgatcaac cgcccgcaca
     3961 ccgtatcgcc ggtggccgac agcagaattt cgcccttctc gtcgaggact gcgctcgcgg
     4021 tgccgtcacc ggcgacttcg cgcagcggcg ccaacactgc ggccgtcggc ggcggaccgc
     4081 cggcattgcg cacgagcatc gcgcgaagcc cctcacgcac cccgcgcacc aaggcgaggt
     4141 cgtcggcccg gcactcggaa tcgggacgca gcaggccatg ggccaccaac caggatctgg
     4201 cctcggctga gtcggcgaga cggtcttggc cttctggcat ctcgattgtg ttgacgagtg
     4261 cctgtatacg gctgagcggc tcgggggccg gcttggactc gtcgtcgccc agccaatgcg
     4321 aagcaggcat atcaccacta tagaacctcg tgaccggtaa atcaatttga ctagtcatgc
     4381 atgaccggtg tagcgttttc gacagtcata agtacggaac cgatgtgagc ggaaagaggt
     4441 ctaccgacat gagcgcgaac cggatttcga tcagcttcct ttcggccgcc cagctgcagg
     4501 atgcaaggcg gcgcgaggag atgtcgcgcg cccctttgaa cgcacgcctg actgcccggc
     4561 tgttcgccac caggttcgac cggatgctcg ccgtcggtgt cagcgctccc gcgggcagcg
     4621 cactcgccgt gcacgcggag cggttgacgt cggtcgacga acgcgaggcg ctggcccgct
     4681 cgctgcgccg ctcggtcggc gacgcccgca accgctacgc gcggatgtcg tcgcgcgtac
     4741 cgctgaacat cccgaacatc accgcggcgc aggaccgaat cgaccaggtc acgctgcgac
     4801 tgcactcgcc gcggccggtg agcgcacgcg gaatcgctcg tctgcgggtg ctgctcgccg
     4861 acgggaccgg gccgatgtac cgctacggcc gcggagacct cgaggggcgc ctcggggcgg
     4921 cgctcgcggc tctgtgaacc ggcgtcgcgc tacacccgca gcacggcggc gcccgcgatg
     4981 cgcccggcgc tgagatcggc gagtgcgtcg cccgcgcggt cgagcggata ctccggtgtc
     5041 gtgaccgcga tgcgatggcg cccggcgaag gcgagaaatt cgcgggcgtc gtcgcgggta
     5101 ttggacgtca ccgagcgcac ctgacgctcg ttgaacaggt gacggtcata gttcaacgcc
     5161 gggatgtcgc tcaggtagat accggcgatc gccagcgtgc caccacgatc gagcgcctcc
     5221 agcgcgggca acaccaggtc gccaacgggt gcgaacagga tcgcggcatc caacttcacc
     5281 ggcggtgggt cgtaggcacc ttgcgccgag gccgcgccga gtgcgagtgc caactcctgc
     5341 gcaaccgcgc cgcgcgtcat cacgtgcact tcggcgccct gcgccagcgc cacctgggcg
     5401 gtgatgtgtg cgctgccgcc gaacccgtag atgcccagcc gcccgccgtg cggaagatcc
     5461 gcgcgcagca aggaccgata gccgatgatg cccgcgcata ataggggggc caattcgttg
     5521 tcggaatacc cgatcggtag cgggtgcgcg aaagccgcag gagccgtagc gaattcggcg
     5581 tatcccccgt cggcgtccca gcctgtgtag cgcgactgcg ggcacaggtt ctcgtcgccg
     5641 cggatgcaga acgtgcactg cccgcaggta tggcgcagcc acgcaacccc gacgcgatcg
     5701 ccgactgtga actcgtcgcc ggcatcttgg cccaccgcaa cgacttcggc gaccacttcg
     5761 tgtccgggta tgacgttcgg ccggtgaacc ggcagatctc cctcggcgac atgcagatcg
     5821 gtgcggcaca caccacatgc ccgcacggca acgagaattt cgccctggcc gggtgacgga
     5881 accgcaacgg tgacgcgttc caacggacgg gtactcatcg ggccggggtc gcggacctgc
     5941 cacgcggtca tcgacttggc agaagtcatg ctcgacgaac gttccttacg agtcgagatt
     6001 tcgcccaatt gtcagccggt acgaaacgtt cggccgtttc gcctagttct tgcgttgcac
     6061 catcccgacg atccacagca ggatcaccga tcccaggatc gcggtgaaca gcgtgaacca
     6121 ccatccgccc gcggcggtgt cgacgaagaa gctgagcagg aagccgccga tcagcgcgcc
     6181 gatgataccg atgacgatat tcatcagaat gcctgagccg ccgcccttga cgatcttgcc
     6241 ggcgatccat cccgcgattg ctccgatgat gatgtagccg atccagccaa cgctggtcag
     6301 tgtcgttgac cgagcggcca actcggtcgc tgtaatggcg tccatctccg gatctcctgc
     6361 ctgtcggcgc ccagcgcgac cgctgggcca acctccggta tacgcccgaa gcgtaacgat
     6421 tcaagggtcg aatcggctac gaacaagatc aaaacggcag gtaacgaaaa cgttctcgtt
     6481 cgtcagcccg cgggcatcat gccgggtgcg ttggccgggt cgacggggac cccgactccg
     6541 accggcggac gtcccggcgc attgttcggc gcgggcggag cgttcgggtc cgcgggagcg
     6601 ggcgcattcg gatccggctc gggcggtggc ggcgggctcc acggccggat cgaattcgcg
     6661 agcgcggcgg ccgcgccctt gtcgacgggg ttgttggcgg tgcccagcca gacgacgaac
     6721 caccgctcgt tgcgctggcc gcgtggagcc agcgactggc caggggccac ggtcggcgac
     6781 gcgttaccga ccacgccggc ccagatctgg ccggtcggct tgttcgtgtc ggtgaacttc
     6841 acgtcgtacg cggagaaggc gcccggcata tcgcccgcgg cgagtgcccc gctttcctgg
     6901 ttgacgcggg tgccggggaa cggcatgaag aactcgccca tatcggaggc cagccgggtg
     6961 gccgccttgc cgttgtcggc ttcggcgcct gcgaacaact tcatgtccag cctgccgagc
     7021 agaacgctgg tgtcgttggc gggctgcgca ccctgctgtc cgggctgagt cgccggggcc
     7081 gg
//
LOCUS       DASYLX010000361         5803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3747173,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000361 DASYLX010000000
VERSION     DASYLX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747173"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1573)
                     /locus_tag="VGP27_08885"
     CDS             complement(80..1573)
                     /locus_tag="VGP27_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6779 domain-containing protein"
                     /protein_id="HEV7851463.1"
                     /translation="MTVLTRGARPRRSGRRPGWLLLTVLLVLAIMASSALVFTDRVEL
                     LKLAVILSLWAAVVAAFVSVIYRRQSDVDQAKARDLKLVYDLQLDREISARREYELTV
                     ESELRRELSTEVRAQAADEVAALRAELAALRANLEYLFDADLSHRPAIETEHGPARPG
                     RVASSRIDTDDGPNLRTEENPIIDVSTEPHSPEHEWGPTPLFGGAHRRESEALPTTEE
                     RQAWAAEQRAAEHRAAEERAAAEQRAAADQRAAEQRAAAEARAAELRAAEQRAAQQRA
                     AEQHAAAERRAAQRRADEQRAAEQRAADERAAEERHAAAQQAAEERAAAERRIAERRA
                     AEEDALAEQRAAAQRAAEEEADRQAAVADDWRPAPAEGEFIPAGMPGSNWSPSSMENG
                     SGEYVGRRRAAEPAMSTPPYDPQAGRHWAPPESTDPGRPKAPAPSPTLATPARPPAPQ
                     PAPDDDSAPRHRSLDEAESTGGHSVAELLARLEVGSSGGGRRRRRDE"
     gene            complement(1650..2126)
                     /locus_tag="VGP27_08890"
     CDS             complement(1650..2126)
                     /locus_tag="VGP27_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEV7851464.1"
                     /translation="MGPTRRRDLTAAAVVAAVLGYALVTILYRWFPPITVWTGLSLLA
                     VAVAEGGWAFYVRAKINDGRIGDGPGRLHPLAVARSVTIATASAWVGDLVLGWWVGVL
                     VYLLPRRDSLRVAGEDTAGAVVAAVSALALLVAALWLQHCCKSPQEPPENGEGAAD"
     gene            complement(2126..2638)
                     /gene="folK"
                     /locus_tag="VGP27_08895"
     CDS             complement(2126..2638)
                     /gene="folK"
                     /locus_tag="VGP27_08895"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV7851465.1"
                     /translation="MTSVVLSIGSNLGDRLAHLQSVVDGLGDALRTASAVYETDAWGG
                     VEQGPFLNGVLIADDPDLDAHGWLRRAHEFEDAAGRVRDQRWGPRTLDVDLVTCHDGR
                     SEVRLGGENLTLPHPFAHQRAFVLVPWLDVDQDAALTVGGESRPVAKLLAELDPAERD
                     GVRPTGLVLT"
     gene            complement(2635..3018)
                     /gene="folB"
                     /locus_tag="VGP27_08900"
     CDS             complement(2635..3018)
                     /gene="folB"
                     /locus_tag="VGP27_08900"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEV7851466.1"
                     /translation="MADRIELRGLLVRGHHGVFDHERRDGQDFVVDITVWIDLADAAA
                     SDDLADTLDYGALAKRAADIVEGPARNLIETVAGEIAEDVMTDERVHAVEVVLHKPAA
                     PIPLRFADVAVVARRSRRGGRGNSA"
     gene            complement(3011..3844)
                     /gene="folP"
                     /locus_tag="VGP27_08905"
     CDS             complement(3011..3844)
                     /gene="folP"
                     /locus_tag="VGP27_08905"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV7851467.1"
                     /translation="MSPTRVRVMGVVNVTDDSFSDGGLFLDRDRAVEHGLALAADGAA
                     IIDVGGESTRPGAARIESRVETGRVLPVIKELAGQGITVSIDTMHADVAQAALENGAQ
                     IVNDVSGGRADANMAGLVADAKVPWVLMHWRSVVAERPHEVPPYGDVVAEVRAELLSC
                     VDAAVHAGVDPANVIIDPGLGFAKTGEHNWALLRALPELVGTGIPVLIGASRKRFLGT
                     LLADAEGEPRPPDGRETATAVVSALAGMHGAWGVRVHDVRASVDALKVVEAWTEPQRG
                     G"
     gene            complement(3841..4449)
                     /gene="folE"
                     /locus_tag="VGP27_08910"
     CDS             complement(3841..4449)
                     /gene="folE"
                     /locus_tag="VGP27_08910"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV7851468.1"
                     /translation="MTRPHNNSATTNGRLFDQARAEAAVRELLIAVGEDPDRHGLEET
                     PARVARAYKEMFAGLYIDPDSVLNTTFDEQHDELVLVKQIPLYSTCEHHLVAFHGVAH
                     VGYIPGVDGRVTGLSKIARLVDLYAKRPQVQERLTAQIADAVMRKLEPRGVIVVVECE
                     HLCMSMRGVRKPGATTTTSAVRGQFKTDKASRSEALELILRR"
     gene            complement(4466..>5803)
                     /locus_tag="VGP27_08915"
     CDS             complement(4466..>5803)
                     /locus_tag="VGP27_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEV7851469.1"
                     /translation="GLAKRTVGMSGADLANVINEAALLTARENGTVISGAALEEAVDR
                     VVGGPRRKSRIISEHEKKITAYHEGGHTLAAWAMPDIDPIYKVTILARGRTGGHAMSV
                     PEDDKGLMTRSEMIARLVFAMGGRAAEELVFREPTTGAVSDIEQATKIARAMVTEYGM
                     SSKLGAVRYGTEHGDPFLGRTMGTQADYSHEVAQIIDDEVRKLIEAAHTEAWEILTEY
                     RDVLDTLAGELLEKETLHRAELQAIFGDVKKRPRLTMFDDFGGRIPSDKPPIKTPGEL
                     AIERGEEWPKPVPEPAFKVAIAKASAAAAEASQANGSNGAGTNGSPNGPTQPDYGAPA
                     GWHAPGWPPQHPQQPQGQYPPQGHWYPPPPQPSGWHPPNAAGAAPPPNGAAGAAPPPN
                     VAAGAAPPPYPPYQPYPYPGPQHPSHGGPTGAPPPDNEDAGQESNRPNPPAHG"
BASE COUNT          800 a         2052 c         1966 g          985 t
ORIGIN      
        1 cattgcagag ggcctcgcga gatctgtgtt tcgttccggt cctgcgtcgc gggtaccgaa
       61 cggtcgctaa gagactagct cactcgtcgc ggcgtcgacg acgcccgccg cccgacgaac
      121 cgacttccag acgcgccagc aactcggcga cggaatgccc gccggttgac tccgcttcgt
      181 cgaggctgcg gtgacgaggt gcgctgtcat cgtcgggtgc cggctgcgga gcgggcggcc
      241 gagcaggtgt cgcgagtgtg ggtgacggtg ccggtgcttt tggtcgtccg gggtcggtgg
      301 actcgggcgg cgcccagtga cggcccgcct gcgggtcgta cggaggtgtc gacatcgcgg
      361 gctctgcagc gcgtcgccgt ccgacgtatt cgccggatcc gttctccatc gacgagggag
      421 accaattgct gccgggcatt ccggccggga tgaactcgcc ctcggcaggg gcgggccgcc
      481 agtcgtctgc gaccgcggcc tgcctgtcgg cttcctcttc tgcggcacgc tgcgcggccg
      541 cccgctgttc ggcaagtgcg tcttcctcgg ccgcccggcg ctcggcgatc cgacgctcag
      601 cggccgcgcg ctcctcggcc gcctgttgcg ctgccgcgtg tcgctcctcg gcggcgcgct
      661 cgtcggccgc gcgttgttcc gcagcgcgct gttcgtcggc gcgccgctgc gcggcacgtc
      721 gttccgccgc ggcgtgctgt tcggcggcgc gctgctgcgc cgcccgctgt tcagccgctc
      781 tcagctcagc cgccctggcc tcggcagcag cgcgctgttc ggccgcccgt tgatctgctg
      841 cggccctctg ctccgccgcg gcgcgttcct ccgcggcacg gtgctctgca gcccgctgct
      901 cggctgccca cgcctggcgc tcctcggtcg tcggcagcgc ctccgattcc ctgcggtggg
      961 caccaccgaa cagcggcgtc ggcccccatt catgttccgg cgaatgcggt tcggtcgaaa
     1021 cgtcgatgat cggattttct tcggtgcgca gattcgggcc gtcgtcggtg tcgattctgc
     1081 tgctggccac ccggccgggg cgcgcgggcc cgtgttccgt ctcgatcgcc gggcggtgac
     1141 tgaggtcggc gtcgaagagg tactccaggt tggcccggag ggcggcgagc tcggcgcgca
     1201 gggcggccac ctcgtcggcg gcctgggccc gtacctcggt cgacagctcg cggcgcagct
     1261 cggactcgac cgtcagctcg tattcgcgcc gtgcagagat ctcacgatcc agttgtaagt
     1321 catagacgag cttgagatcc cgggccttcg cctgatcgac atcgctctgg cgccgataga
     1381 tgaccgacac gaacgcggcc accaccgcgg cccacaacga gaggatgacc gccagcttca
     1441 gcaattccac ccggtcggtg aacaccagtg ccgagctcgc catgattgcg aggaccagca
     1501 acaccgttaa cagcagccaa cccggcctgc gaccgctgcg ccgcggccgc gcaccacggg
     1561 tcagaacggt catgggccga ctgtacccgt cacaggtcac tcagcgtgtc gtgctctgcg
     1621 gcgattcgcg atcggctatt gtcgaccagc taatccgcgg ccccttcacc gttctccggc
     1681 ggctcctgcg gggacttgca gcaatgttgg agccatagcg ccgcaaccaa cagcgcaagc
     1741 gcgctcaccg ccgccaccac agcccccgcc gtatcctctc cggccacccg gagcgaatct
     1801 cgccgtggca aaaggtagac cagcaccccg acccaccaac ccagcaccag gtcgccgacc
     1861 cacgccgagg ccgtggcgat cgtcaccgac cgggcgaccg ccagcggatg caaccgaccc
     1921 ggcccgtcac cgatccgacc gtcgttgatc ttcgcccgca catagaacgc ccagccgccc
     1981 tcggcgaccg ccaccgccag cagcgacagg ccggtccaga ccgtgatcgg cgggaaccac
     2041 cggtagagga tggtcaccag tgcgtatccc aggacagcgg cgaccaccgc agcagccgtc
     2101 aggtcacgcc tgcgggtcgg tcccatcagg tcagcaccag gccggtcgga cgaaccccgt
     2161 cgcgttcagc cgggtcgagt tcggcgagca gcttcgccac gggtcggctt tcgccgccca
     2221 cggtcagcgc ggcgtcctgg tcgacgtcca gccagggaac gagaacgaag gcgcgctggt
     2281 gtgcgaacgg atggggcagt gtcaggttct cgccgccgag tcgcacctcg gagcgtccgt
     2341 catggcaggt gacgagatcc acatcgaggg tccgcggacc ccatcgctga tcgcggaccc
     2401 gcccggcggc gtcttcgaac tcgtgcgccc ggcgcagcca gccgtgggca tcgaggtccg
     2461 gatcgtcggc gatgagcacg ccgttgagga acggcccctg ctcgacgccg ccccacgcgt
     2521 cggtctcgta gacggccgac gccgtgcgga gcgcatcccc gagtccgtcg acgaccgact
     2581 gcaggtgggc gagccggtcg cccaggttcg aaccgatcga cagcaccact gaggtcacgc
     2641 tgaattcccc cggccgccgc gccgtgaccg tcgtgccacc acggcgacgt cggcgaaccg
     2701 cagcggaatc ggggcggccg gtttgtgtag gacgacctcg actgcgtgca cccgctcgtc
     2761 ggtcatcacg tcctcggcga tctcgcccgc aaccgtctcg atcagattgc gtgccggtcc
     2821 ctcgacgatg tcggcggcgc gcttggccag agcgccgtag tcgagcgtgt cggcgaggtc
     2881 atcgctggcc gccgcgtccg caagatcgat ccacaccgtg atgtcgacga cgaagtcctg
     2941 gccgtcgcgg cgctcgtggt cgaacacacc gtgatgaccg cgaacgagca acccgcgcaa
     3001 ttcgattcgg tcagccaccg cgctgcggct ccgtccatgc ctcgacaacc ttcagcgcgt
     3061 cgaccgacgc tcggacatcg tgcacccgga caccccacgc gccgtgcatg cctgccagtg
     3121 ccgagaccac cgcggtcgcg gtctcgcggc cgtccggcgg acgcggttcg ccctcggcgt
     3181 ccgcgagaag ggttccgaga aagcgtttgc gcgacgcgcc gatgagcacc gggattccgg
     3241 tgccgacgag ttcgggcaat gcgcgcagca gcgcccagtt gtgctcgccg gtcttggcga
     3301 atcccagccc cgggtcgatg atgacgttgg ccgggtcgac tcccgcgtgc actgcggcat
     3361 cgacgcagga gagcagttct gcgcgcacct cggccaccac gtcgccgtac ggcggcacct
     3421 cgtggggacg ctcggcaacc accgagcgcc aatgcatcag tacccaaggc actttggcgt
     3481 cggccaccag gccggccatg ttcgcgtcgg cgcgcccgcc cgacacgtcg ttgacgatct
     3541 gcgcaccgtt ttccaatgca gcctgggcaa cgtctgcgtg catcgtgtcg atgctgacgg
     3601 taatgccctg tccggcaagc tctttgatca ccggcagcac acgcccggtt tccacccgcg
     3661 actcgatgcg ggcggcaccg ggacgcgtcg actcgccgcc gacatcgatg atcgccgcgc
     3721 cgtcggcggc cagcgccaga ccgtgctcga cggcgcgatc gcggtcgagg aacagccctc
     3781 cgtcggagaa ggagtcgtcg gtgacgttca cgacccccat gacccgcacg cgtgttgggc
     3841 tcacctgcgc agaatcagtt ccagtgcctc ggaccgtgat gccttgtcgg ttttgaactg
     3901 accgcgcacc gccgatgtgg ttgtggtggc gcctggcttg cgcacgccgc gcatggacat
     3961 gcacaggtgc tcgcattcga cgacaacgat gactccacgc ggttcgagct ttcgcatgac
     4021 ggcgtcggcg atctgtgcgg tcaggcgctc ctgcacttgc gggcgcttgg cgtacaggtc
     4081 gaccagcctg gcgatcttcg acagcccggt cacgcggccg tccaccccgg ggatgtatcc
     4141 gacgtgggca acgccgtgga acgccaccaa gtggtgctcg caggtggagt acaacgggat
     4201 ttgcttcacc agcaccagct cgtcgtgctg ttcgtcgaat gtggtgttaa gcaccgaatc
     4261 cgggtcgatg tagagcccgg cgaacatctc tttataggca cgcgctaccc gggccggagt
     4321 ctcctccagg ccgtgccggt cggggtcctc cccgaccgct atcagcagtt cgcggacggc
     4381 cgcctcggcg cgcgcctggt cgaaaagacg tccattggtg gtcgcagagt tgttgtgcgg
     4441 ccgcgtcatt gagcctccgt tgggttcagc cgtgcgccgg cgggttaggc cggttcgatt
     4501 cttggcccgc gtcctcgttg tcaggcgggg gcgctcctgt cggtccgccg tgcgacgggt
     4561 gttgcgggcc ggggtacggg tagggctgat acggcgggta gggcggcgga gccgctccgg
     4621 cggcgacatt gggcggcgga gccgctccgg cggcgccatt gggcggcgga gccgctccgg
     4681 cggcattggg cggatgccat ccgctgggct gcggcggcgg cgggtaccag tggccctgcg
     4741 gcgggtattg gccctgcggc tgctgcggat gctgcggcgg ccagcccggc gcatgccagc
     4801 ctgcaggtgc gccgtagtcg ggctgcgtcg gaccgttcgg acttccgttg gtgcctgcac
     4861 cattggagcc gttcgcctga gatgcttctg cggcggcggc ggaagccttg gcgatggcga
     4921 ccttgaacgc aggctccggg actggcttcg gccactcctc gccgcgctcg atcgccagct
     4981 cgcccggcgt cttgatcggc ggcttgtcgg acgggatgcg accgccgaag tcgtcgaaca
     5041 tggtcagccg cgggcgcttc ttcacgtcgc cgaagatcgc ctgcagttcc gcgcggtgca
     5101 gcgtctcctt ctccaaaagt tcgcctgcca acgtgtcgag cacgtcgcgg tactccgtga
     5161 ggatttccca cgcctcggtg tgcgcggcct cgatgagctt gcgcacctcg tcgtcgatga
     5221 tctgcgcgac ctcgtggctg tagtcggcct gcgtgcccat cgtgcggcca aggaacgggt
     5281 cgccgtgctc ggtgccgtaa cgcacggcgc ccagcttgct gctcatgccg tactcggtga
     5341 ccatcgcgcg ggcgatcttg gtggcctgct cgatgtccga aacggcgccg gtagtgggct
     5401 cgcggaacac cagctcctcg gctgcgcgtc cacccatcgc gaacaccagc cgcgcgatca
     5461 tctccgagcg agtcatcagg cccttgtcgt cctcgggcac cgacatcgcg tgcccgccgg
     5521 tacgaccgcg ggccaggatg gtgaccttgt agatcgggtc gatgtcgggc atcgcccacg
     5581 ccgcgagcgt gtggccgcct tcgtggtagg cggtgatctt cttctcgtgc tcgctgatga
     5641 tgcggctctt gcgccgcggc ccaccgacga cgcggtcgac ggcctcctcg agcgcagctc
     5701 cgctgatgac agtgccgttc tcgcgggcgg tcagcagcgc agcttcgttg atgacgttgg
     5761 ccaggtcggc gcctgacatg ccgacggtgc gcttggccag tcc
//
LOCUS       DASYLX010000362         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3747223,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000362 DASYLX010000000
VERSION     DASYLX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747223"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGP27_08920"
     CDS             complement(<1..315)
                     /locus_tag="VGP27_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7851470.1"
                     /translation="MAEGSPPQSSGLAAELAAAGFTDAVEVGRGGGGVVFRCYQKSLG
                     RSVAIKVLASGLDEDDRERFLREGYAMGALSGHPNIANILQVGMTESNRPFIVMPLST
                     MGS"
     gene            445..900
                     /locus_tag="VGP27_08925"
     CDS             445..900
                     /locus_tag="VGP27_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7851471.1"
                     /translation="MAGKYATADAVGLPELLEFVRPRHHMVLTTFRADGSLQSSPVTG
                     GVDEHGRIVIASYPQRAKSANIRRTPRASVTVMSDEFNGPYVQVDGDAEVIDLPDALE
                     PLVDYFRSISGEHSDWNEYRQAMVDQGKCLIRVTPRRWGPVATGGFPRP"
     gene            1110..2345
                     /locus_tag="VGP27_08930"
     CDS             1110..2345
                     /locus_tag="VGP27_08930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7851472.1"
                     /translation="MRVALLSYRSKTHCGGQGVYVRHLSRGLVELGHDVEVFSGQPYP
                     GGLDPRVRLTKVPSLDLYREPDPFRIPWPNEIKTTIDLLELLTTWTAGFPEPRTFSLR
                     AARVLARRLADFDVVHDNQCLGSGLLKIADLGLPVVATVHHPITRDRVLDVAAARWWR
                     KPLVRRWYGFAEMQKDVARRIPELLTVSSTSAADIAEDFAVSSDQLHVVPLGVDTALF
                     KPAEKRVRNRVIAIASADVPLKGVSHLLHAVARLRVERDLELQLVAKLEPNGPTEKLI
                     AELGISDIVHSSSGLSDSELADLLASAEVACIPSLYEGFSLPAVEAMASGTPIVASRA
                     GALPEVVGSDGECARLVTPADVDELTDVLGELLDSPLERRRLGANGRQRALDVFSWES
                     VAAQTVAVYEMAMARVAQC"
     gene            2339..3070
                     /locus_tag="VGP27_08935"
     CDS             2339..3070
                     /locus_tag="VGP27_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851473.1"
                     /translation="MLTVDYDRLGVGAGTKVIDVGCGAGRHSFEAFRRGADVVAFDQN
                     ASDLNDVDEILQAMKEQGQAPASAKAEAVKGDALDLPYGDGIFDCVIASEILEHVPED
                     DRVISELVRILKPGGALAITVPRWLPERVCWALSDEYHANEGGHIRIYRADALRDKVL
                     AHGLKITHSHHAHALHSPFWWLKCAVGTSKSDHPAVAAYHKLLVWDMMSRPWLTRTAE
                     SLLNPLIGKSVALYFRKPEVPVASD"
     gene            3066..>3174
                     /locus_tag="VGP27_08940"
     CDS             3066..>3174
                     /locus_tag="VGP27_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEV7851474.1"
                     /translation="MDKLDVPAVPGVLTPAQCRQTAQSIANTQESSGAIP"
BASE COUNT          517 a         1035 c         1068 g          554 t
ORIGIN      
        1 ggaacccatg gtgctcaacg gcatcacgat gaacggccgg ttgctttcgg tcatcccgac
       61 ttgaaggatg ttcgcgatgt tggggtggcc ggaaagcgct cccatcgcgt agccctcacg
      121 cagaaagcgc tcccggtcgt cttcgtcgag accagaggcg agcaccttga tcgcgacgct
      181 ccggccgagg gatttctggt agcaccgaaa gacgacgccc ccgccgccgc gacccacctc
      241 gacggcgtcg gtgaacccgg cggctgccaa ctcggcagct agcccgctcg attgcggggg
      301 agagccttcc gccatggttc cgaccgtcct ccggtgggat gggagtgatt cgtaagctgt
      361 aacggtaccg agcaaactgc agttcaacgt gggattcgcg ctcgtgggcg cggggcaatc
      421 gatggttctg gctaggctgg tcgggtggcc ggaaaatatg ccaccgcaga tgcggtcggg
      481 ctacccgaac tgctcgagtt cgtccgcccg cggcatcaca tggtgctgac gacgtttcgc
      541 gccgacgggt cgctgcagag ctcgccggtg accggcggtg tcgacgaaca cggtcgcatc
      601 gtcatcgcca gttaccctca gcgggcgaag tcggccaata tccgtcgcac gccgcgcgcg
      661 agcgtgacgg tgatgtcgga cgaattcaac ggcccctatg tccaggtcga cggcgacgcc
      721 gaggtgatcg acctgcccga tgccctcgag ccgctggtcg actacttccg gtcgatatca
      781 ggcgaacatt cggactggaa cgaataccgg caggcgatgg tcgaccaggg caagtgcctg
      841 atccgcgtca ctccgcgacg ctggggtccg gtcgccacgg gtggctttcc gcggccctag
      901 cgagtcgggc tgggcgcgcc ggtcggccgt gactgtggta gtcaggcagg gtggccgcgt
      961 tgcgtcagat agaccgtcaa actatagtct ggacacatgt ccagtagcct gtcgccgtct
     1021 cgggccgcag gtcaagaggg taagcaggct gtttgtcgac tcctcgcgca agcgctcgtc
     1081 ggccctgaag tccggagagt gaactgagta tgcgcgttgc cctgctgtct taccggagca
     1141 agacgcactg cggtggacag ggcgtctatg ttcgccatct gagccggggg ctcgtcgagc
     1201 tcggccacga cgtcgaagtc ttctcgggtc agccctaccc gggcggcctc gacccgcggg
     1261 tccggctcac caaggtgccc agcctcgacc tataccgcga gcctgacccg ttccgcatcc
     1321 cgtggccgaa cgagatcaag accacgatcg acctgctgga gctgttgacg acgtggacgg
     1381 ccggcttccc ggagccgcgg acgttcagcc tgcgtgcggc gcgggtgctg gcccggcgcc
     1441 tggccgactt cgacgtcgtg cacgacaacc agtgcctcgg ctcaggcctg ctgaagatcg
     1501 ccgacctggg gctgccggtg gtggcgacgg tgcatcatcc gatcacccgc gaccgggtcc
     1561 tcgacgtcgc cgccgcgcgg tggtggcgca agccgctggt gcgccgctgg tacggcttcg
     1621 ccgagatgca gaaggatgtc gcgcgccgga ttcccgagct gctgaccgtg tcgtccacct
     1681 ccgccgctga tatcgccgag gacttcgcgg tgtcgtccga ccaactgcac gtcgttccgc
     1741 tgggagtcga caccgcactg ttcaagcccg ccgagaaacg ggtgcgcaac cgcgtcatcg
     1801 cgatcgcgag cgccgacgtc ccgctcaagg gcgtgagtca tcttctgcac gcggtggcac
     1861 ggcttcgcgt ggagcgcgac ctcgagctgc agctggtcgc caagttggag cccaacggcc
     1921 cgaccgagaa gttgatcgcc gaactcggca tctccgacat cgtgcacagc tcaagcggcc
     1981 tgtcggactc cgagctcgca gacctgttgg cgtctgccga agtagcttgt atcccttccc
     2041 tttacgaggg cttctcactg cccgcagtcg aggcgatggc cagtggcacg ccaatcgttg
     2101 cgagtcgggc cggcgcgctg cccgaggtgg tcggctccga tggtgagtgc gcacggctgg
     2161 tcacccctgc cgacgtcgac gagctcaccg acgttctcgg cgaactgctc gactcgccgc
     2221 tggaacggcg tcgactcggc gcgaacggcc gccagcgcgc gctcgacgtt ttcagctggg
     2281 aatccgttgc ggcacagacg gttgcggtgt acgagatggc catggcgagg gtcgcgcagt
     2341 gctgacggtc gactacgacc ggttgggagt cggcgcgggc acgaaggtga tcgacgtcgg
     2401 ctgtggtgcc ggtcgccatt cgttcgaggc gttccggcgc ggagcggacg tggtcgcctt
     2461 cgatcagaac gcgtccgatc tcaacgacgt cgacgagatc ctgcaggcga tgaaggagca
     2521 gggccaagcg cccgcgtcgg ccaaggccga ggcggtcaag ggggatgcac tcgacctgcc
     2581 ttacggcgac ggcatattcg actgcgtcat cgcctccgag atcctcgagc atgtccccga
     2641 ggacgaccgg gtgatctctg aactggttcg cattctcaag ccgggcggcg cgctcgcgat
     2701 caccgtgccg cggtggctgc ccgaacgggt gtgctgggcg ctgtcggatg agtaccacgc
     2761 caacgagggc gggcacatca ggatttaccg cgccgacgcg ctgcgcgaca aggtcctcgc
     2821 ccatggcctg aagatcaccc acagtcacca tgcgcacgcg ctgcactcac cgttctggtg
     2881 gctgaaatgc gctgtcggaa cctcaaagtc ggatcatccc gcggtggcgg cctaccacaa
     2941 gttgctggtg tgggacatga tgagccggcc ctggctgacg cgcaccgccg agtcgctgtt
     3001 gaacccgctg atcggcaaga gcgtggcgtt gtacttccgc aagccggagg ttcccgttgc
     3061 ttcggattga caaacttgac gttcccgcgg tgccgggcgt cctgacacct gcgcagtgca
     3121 ggcagaccgc gcagtcgatc gcgaacaccc aggagtcctc cggcgccatc ccct
//
LOCUS       DASYLX010000363         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3197123,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000363 DASYLX010000000
VERSION     DASYLX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197123"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1834
                     /locus_tag="VGP27_08945"
     CDS             35..1834
                     /locus_tag="VGP27_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7851475.1"
                     /translation="MKSVLLSRRDLDFLLFEWLRVHELTNRERFAEHSRETFDAVLDL
                     CEQLAARYFATHNKTSDANEPTFDGEKVTIIPEVKEAWDAFAQADLLAMSMDQRLGGS
                     QLPATVAQAGFAWFSAANVSTTGYLMLTMANANLLARFGAPELIDTFLQPMLTGRFSG
                     TMALSETQAGSSLADILTRAEPQDDGTYRLFGSKMWISGAEHELTDNIVNLVLAKIPG
                     APPGTKGISLFIVPKYLVNTDGSIGERNDVVLAGLNHKMGQRGITNTVLSFGDGTYSP
                     DGKPGAVGYLVGEQHRGIAYMFTMMNEARLGVGMGAVALGYTGYLKSVAYARERPQGR
                     PVTAKDPSAPQIPIIEHADIKRMLLAQKAYVEGGMALLLYCAKLVDLQHSAESDNERD
                     HATLLLDMLTPVGKSWPSQWCLEANSLAIQVHGGYGYTREYDVEQHYRDNRLNPIHEG
                     THGIQSLDLLGRKVTMRGGAGLAALGDAIGRSVAEAAALKGEPAEQAAQLEATWQRLI
                     AVTTAMFGSGDVEAALANSVIYLEAFGHIVVAWMWLQQVVAAHGRDGNFYDGKRHAAR
                     YFFRYELPKTTPQLDLLQSLDRTTLEMQDAWFG"
     gene            2008..>3116
                     /locus_tag="VGP27_08950"
     CDS             2008..>3116
                     /locus_tag="VGP27_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851476.1"
                     /translation="MESISLSRTQSEAGHLRGSTEYRRLLAALFCAGIATFAQLYSPQ
                     GVLPQISADLGVGAASAALMVSACTIGLAIGVIPWSMIADRVGRVKAMSVSVITSSVL
                     GLLTPFAPSFELLLAGRLLEGLVLGGVPAIAIAYLTEEIVSEHTARAAGTFVSGTSIG
                     GLLGRVIAGPITDIATWRIGMFAVAVLCAAAAMGFVKLAPQSRRFTPSNGRRANPEGS
                     LAHRLLVNLRSPSQLTLFAQGFLLMGGFVAVYNYLAFRLTAAPYHLPVTVVSLIFFAY
                     LAGTGASTVAGAMATRFGRKAVLLTSIAIMITGVLITLCANLFLVLVGLVVGTIGFFG
                     AHAIATGWAGRAATVGKAQASSLYNLFYYAGSSIIG"
BASE COUNT          558 a         1014 c          976 g          568 t
ORIGIN      
        1 gaatgtcccc gtctgtgatg agcagaggac ggttatgaaa tcggtgttgc tgtcgcggcg
       61 cgacttggat tttctgctgt tcgagtggtt acgcgttcac gagttgacga accgggagcg
      121 cttcgcagag cactcgcgag agacgttcga cgcagtactg gacctgtgcg agcaactcgc
      181 ggcacgctac ttcgcgacgc acaacaagac aagcgacgcc aacgagccga ccttcgacgg
      241 cgagaaggtc acgatcattc ccgaggtgaa ggaggcgtgg gacgccttcg cccaagccga
      301 tctgctagca atgtcgatgg atcagcggct gggcggctcg cagttgcccg ccaccgtggc
      361 ccaggccggc ttcgcatggt tctcggcggc caacgtgagc accaccggct acctgatgct
      421 caccatggcc aatgccaacc tgctggcccg gttcggcgca ccggagctga tcgatacgtt
      481 tctgcagccg atgctgaccg gtcggttctc cggcacgatg gcgctgtcgg agactcaggc
      541 gggctcgtcg ctggccgata tcctgacccg cgccgagccc caagacgacg gaacctaccg
      601 gctgttcgga tcgaagatgt ggatctccgg agccgaacac gagctgaccg acaacatcgt
      661 caacctcgtg ctcgcgaaga tccccggggc tccgccgggc accaagggca tttcgctgtt
      721 catcgtgccg aagtacctcg tcaacaccga cggctcgatc ggcgagcgca atgacgtggt
      781 gctggccggc ctgaatcaca aaatgggcca gcggggcatc accaacacgg tgctcagctt
      841 cggcgacggc acctacagtc ccgacggaaa gcccggcgcc gtcggatatc tggtcggcga
      901 gcaacatcgc ggcatcgcct acatgttcac gatgatgaac gaggcgcgct tgggtgtcgg
      961 gatgggcgcg gtggcattgg gttacacggg ctacctgaaa tcggtggcgt acgcccgcga
     1021 gcgcccacag ggccgtccgg taacggccaa ggacccgtcg gctccgcaaa taccgattat
     1081 cgagcacgcc gatataaaac gaatgctgtt ggcgcagaag gcatatgtcg aaggcggcat
     1141 ggcgctgctg ttgtactgcg ccaagcttgt cgacctgcag cacagcgccg agtccgacaa
     1201 cgagcgcgat catgccacgc tgttactgga catgctcaca cccgtcggca agagttggcc
     1261 gtcgcagtgg tgcctggagg ccaacagcct cgccatccag gttcacggtg gatacggcta
     1321 cacccgcgag tacgacgtcg aacagcacta ccgggacaac cggctcaacc ccatccacga
     1381 gggcacgcac ggcatccaga gcctcgacct gttgggccga aaggtaacca tgcgcggcgg
     1441 tgcggggctc gcggctctag gtgacgcgat tggccggagc gtggccgagg ccgccgcgct
     1501 caagggcgag cctgccgaac aggcggccca gctcgaagcg acgtggcagc gcctgatcgc
     1561 ggtcaccacc gcgatgttcg gctcgggtga cgttgaggcc gctctggcca acagcgtcat
     1621 ctatctcgag gcgttcggac acatcgtcgt cgcgtggatg tggctgcagc aggtcgtggc
     1681 ggcgcacgga cgcgatggca atttctacga tggcaagcgg cacgcagcgc gctatttctt
     1741 ccgctacgaa ctgccgaaaa cgacaccaca actggacctt ctgcagtcgc tggaccgcac
     1801 cacgctggag atgcaggacg cttggttcgg ctgacctagt cgcgtcgatt ccacccgccg
     1861 cattgcccat gccttggtga gcaaaatgcg caatacctgt atatcgctgg ttgcgcggct
     1921 tgtgcgcaca acggtgtcac gccgtcggca accacatagt ttggcggcaa tcgatcgcct
     1981 gacccgagac cccacagaag gtgtgccatg gaatccatct ccctcagccg cacgcaatct
     2041 gaggctggcc accttcgggg gtccaccgaa taccgccgtc tgctggcagc attgttctgc
     2101 gcagggatag ccacgttcgc ccagctctat tcgccgcagg gcgttctgcc gcagatctcg
     2161 gccgacctgg gtgtgggcgc ggccagtgcg gcgctcatgg tgtcggcctg cacgatcggt
     2221 ctggccatcg gtgtcatccc ctggtcgatg atcgccgacc gcgtcggccg ggtgaaggcg
     2281 atgtccgttt cggtcatcac gtcgagcgtc ctcggcctgc tcacaccatt cgcaccgagc
     2341 tttgaactat tgctcgccgg acggctgctg gaaggactcg tgctcggtgg ggtgcccgcc
     2401 atcgcgatcg cgtacctcac cgaagaaata gtttccgaac acacggcccg cgcggcgggc
     2461 accttcgtct ccgggaccag catcggcgga ctcctgggga gggtaatcgc cggcccgatc
     2521 accgacatcg caacgtggcg tatcggcatg ttcgccgtgg ccgtgctgtg cgccgccgct
     2581 gcgatgggct tcgtcaaact cgcgccgcag tcacgccgtt tcacgccgtc gaacggacgc
     2641 agggccaacc cggagggcag cctcgcccac cgcctcctcg tcaacctgcg ctcaccgagc
     2701 cagctgacgc ttttcgccca gggttttctg ctcatgggtg gcttcgtggc cgtctacaac
     2761 tacctggcat tccggctgac tgccgcgccc tatcacctgc cggtgacagt ggtcagcctg
     2821 atcttcttcg cttaccttgc aggtaccggc gcatcaacgg tcgcaggcgc catggccact
     2881 cggttcggcc gcaaggcggt tctcctgaca tcgatagcaa tcatgatcac cggagtgctg
     2941 atcacgttgt gcgccaacct gtttctagtg ttggtaggcc tggtggtagg cactatcggc
     3001 ttcttcgggg cccatgcgat cgccaccgga tgggccggcc gcgccgccac cgtcggcaag
     3061 gcgcaggcct cgtcgctgta caacctgttc tactacgccg gatccagcat catcgg
//
LOCUS       DASYLX010000364         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2101290,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000364 DASYLX010000000
VERSION     DASYLX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101290"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGP27_08955"
     CDS             complement(<1..591)
                     /locus_tag="VGP27_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7851477.1"
                     /translation="MPEYDYEEMKKEAEQYIKFEKDKKNRIAYITFDRPDAQNATSLG
                     MRQNYADLIHKCNVDDDVKVVVIRGEGDDFGSGGDLPEQRGMIENPGMPLLHELAIND
                     DEVTYPPGDSYRYLSTVTDFYAKAKAGNRPLQELRKISIIEAKGYCYGWHFYQAGDAD
                     LVISSDDALFGHPAFRYVGWGPRLWWWAETMGLRKFS"
     gene            complement(613..714)
                     /locus_tag="VGP27_08960"
                     /pseudo
     CDS             complement(613..714)
                     /locus_tag="VGP27_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(723..>1731)
                     /locus_tag="VGP27_08965"
     CDS             complement(723..>1731)
                     /locus_tag="VGP27_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851478.1"
                     /translation="GAVLEIIQSGMDIPSGTLIFEDPPIHNIHRKLLSRIFTPRKVAA
                     LEPMIREFTASCLDPLIGTGRFDFVSDLGAQMPMKVIGMLLGIPEADQQHVVDHGDST
                     LRTERGGKMTENPDGVIATGEVFASYIDWRAEHPSDDIMTELLTAEFADETGATRRLS
                     RDELLLYLQVIATAGSETTTRLLGWAGKVLAEHPDQRRDLVANRTLLPLAIEELLRFE
                     PPAPHVSRYVTCDVEYYGQTVCAGSALMLIVGAANRDPRRIGEDSEQFNIYREPLQHL
                     AFGVGTHFCLGNALARLEGRIALDEILDRFPEWQVDLTEAQLSPTSTVRGWESVPAVL
                     P"
BASE COUNT          285 a          549 c          568 g          329 t
ORIGIN      
        1 ggagaacttg cgaagaccca tcgtttcggc ccaccaccac agccgcggac cccagccgac
       61 gtaccggaac gccgggtggc cgaacagcgc gtcgtccgac gagatcacca ggtcggcatc
      121 gccggcctgg tagaaatgcc agccgtagca gtaacccttg gcctcgatga tgctgatctt
      181 gcgcagctcc tgcagcggcc ggttaccggc cttggccttg gcgtagaagt cggtgaccgt
      241 ggacaggtag cggtaggagt cgccgggcgg gtaggtgacc tcgtcgtcgt tgatcgccaa
      301 ctcgtgcagc agcggcatgc cggggttctc gatcatgccg cgctgttcgg gcaggtcgcc
      361 gccgctgccg aagtcgtcgc cctcaccgcg gatcacgacg accttgacgt cgtcgtccac
      421 gttgcacttg tgaatcaggt cggcgtagtt ctgccgcata ccgagtgacg tcgcgttctg
      481 tgcgtccggc cggtcgaacg tgatgtaggc gatgcggttc ttcttgtcct tctcgaactt
      541 gatgtactgt tcggcttcct ttttcatctc ttcgtagtcg tattccggca tgcgcatctc
      601 ctctatctcg tcctacttga gtagacagcc cgcgtccacg ggcagctgaa cgcccgtgac
      661 gtaacgtgat tcgtcagagg cgagaaacaa tacggcgttg ctgatggcct cgacgcgacc
      721 cgtcatggca gcacggcagg caccgactcc cagccacgga cggtggacgt cgggctgagt
      781 tgggcttcgg tcagatcgac ctgccattcc gggaagcggt cgaggatctc gtcgagcgcg
      841 atgcgcccct cgaggcgggc caacgcgttg ccgagacaga agtgggtgcc gacaccgaag
      901 gcaaggtgct gcagcggttc gcggtagatg ttgaactgct cgctgtcctc cccgatccgg
      961 cgcggatccc tgttggcggc gccgacgatc agcatcagcg cgctgccagc gcacaccgtc
     1021 tggccgtagt actccacgtc gcacgtcacg tagcggctga catgcggggc gggcggttcg
     1081 aagcggagca gttcctcgat tgcgaggggc agcagcgtcc tgttggccac cagatcgcgg
     1141 cgctgatcgg ggtgctcggc aagcaccttg cctgcccaac cgagcagcct cgtcgtcgtc
     1201 tcgctgcccg cggtcgcgat cacctgcagg tacagcagca gttcgtcgcg gctcaaccgc
     1261 cgggtggcgc cggtttcgtc ggcaaactcg gcggtcagca attcggtcat gatgtcgtcg
     1321 gacggatgct cggcgcgcca gtcgatgtag gacgcgaaca cctcgccggt ggcgatcacg
     1381 ccgtcggggt tctccgtcat cttgccgccc cgctcggtgc gcagcgtcga gtcgccgtgg
     1441 tcgacgacat gctgctgatc cgcctcgggg atgcccagca gcatcccgat gaccttcatc
     1501 ggcatctgcg cgccgaggtc tgagacgaag tcgaagcggc cagtgccgat caacggatca
     1561 aggcagctcg cggtgaactc gcggatcatc ggttccagcg cggcaacctt gcgcggcgtg
     1621 aagatccgcg acagcagctt gcggtggatg ttgtggatcg gcggatcctc gaagatgagg
     1681 gtgcccgaag ggatgtccat gccggactgg atgatctcca gcaccgcacc a
//
LOCUS       DASYLX010000365         9043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3197202,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000365 DASYLX010000000
VERSION     DASYLX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197202"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGP27_08970"
     CDS             complement(<1..218)
                     /locus_tag="VGP27_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HEV7851479.1"
                     /translation="MEGAVAGLVLVGVLVVFAAIIVAKSIAIIPQAEAAVIERLGRYT
                     KTVSGQLTLLVPFIDKIRARVDLRERVVS"
     gene            complement(250..681)
                     /locus_tag="VGP27_08975"
     CDS             complement(250..681)
                     /locus_tag="VGP27_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEV7851480.1"
                     /translation="MSIALIWLIAALALAGAEALTGDLFLLMLGGGALAAAGSSLVFD
                     ELWVHGAVFAVVSVLLLVMVRPALRRHFMSGTGLPDRPTELEGKSALVLDRVARHEGQ
                     VKLDGEIWTARPVSDDDVYEPGDHVTVVHIDGATAVVQKVV"
     gene            741..1595
                     /locus_tag="VGP27_08980"
     CDS             741..1595
                     /locus_tag="VGP27_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851481.1"
                     /translation="MWCPSVSLSVWANAWLAGAAAPDDVLDALSQWAPRHSVTAYDAV
                     AAESTGLPWPDVNHSGTMSLLQTLRTAAGAVTGGPAMSVVLPVPGDVRGLPAGTQFQR
                     DAVTAGEAIVINSGSSSQTIGIVPDFEYDDTEPYDDSDDAELSGLNWTVYSLPAAPIP
                     EHIDLGEAEFALRSAVRSAADALGAIRAEAGGMDVADPRRLVEQVMESTGQHRAPDHA
                     PSRALRVLENAAHIDAIVTVSSGLMPIGLQSSSEAQSANEALRPLSAVVRSARLAAVG
                     AILHSAWG"
     gene            complement(1592..2635)
                     /locus_tag="VGP27_08985"
     CDS             complement(1592..2635)
                     /locus_tag="VGP27_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV7851482.1"
                     /translation="MDSSDFDALLLLSFGGPEAPEHVMPFLENVTRGRGIPRERLSAV
                     AEHYLHFGGASPINGINRALIEQLEAELADRGLALPVYFGNRNWEPYVEDAVTAMRDN
                     GVRRAAVFTTSAWGGYSSCTQYNEDIARGRQAAGERAPQLVKLRQYYDHPLFVEIFAE
                     AIGDAAQALPAELRADARLVFTAHSIPFAARSRCGDDLYARQVAYASHLVAAEAGYAD
                     YDQVWQSRSGPPQVPWLEPDVGDHLSALADAGTKAVIVCPIGFVADHIEVVWDLDNEL
                     RRQADELGVALARAATPNADRRFARLAIDLIDELREGSDPSRTESPPGVEPPPLYGFS
                     VDGALCTPECGKS"
     gene            complement(2639..3448)
                     /gene="inhA"
                     /locus_tag="VGP27_08990"
     CDS             complement(2639..3448)
                     /gene="inhA"
                     /locus_tag="VGP27_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent enoyl-ACP reductase InhA"
                     /protein_id="HEV7851483.1"
                     /translation="MAGLLEGKRILVTGIITDSSIAFHIARVAQESGAQLVLTGFDRM
                     KLIGRIADRLPEKAPLLELDVQNEEHLATLADRVTEVIGADNKLDGVVHSIGFMPQSG
                     MGINPFFDAPYEDVSKGIHISAFSYASLAKATLPIMNAGGSIVGMDFDPTRAMPAYNW
                     MTVAKSALESVNRFVAREAGQFGVRSNLVAAGPIRTLAMSAIVGGALGADAGDQIRLL
                     EEGWDQRAPVGWNMKDPTPVARTVCALLSDWLPATTGTVVYADGGAHTQLL"
     gene            complement(3477..4241)
                     /gene="fabG1"
                     /locus_tag="VGP27_08995"
     CDS             complement(3477..4241)
                     /gene="fabG1"
                     /locus_tag="VGP27_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG1"
                     /protein_id="HEV7851484.1"
                     /translation="MTHSAVPETADQKAGGRPPFVSRSVLVTGGNRGIGLAIALRLAA
                     DGHKVAATHRGSGVPDGLFGVECDITDSAAVDRAFTEVEESQGPVEVVIANAGVAADA
                     FLMRMTEEKFEKVIDANLTGAFRVAQRASRSMLRKRFGRMIFVGSVSGTWGVGNQANY
                     AASKAGLIGMARSIARELTKAGITANVVAPGYIDTDMTRALDERIQEGALGFVPAKRV
                     GTAAEVAGVVSFLASEDASYISGAVIPVDGGMGMGH"
     gene            complement(4336..5343)
                     /locus_tag="VGP27_09000"
     CDS             complement(4336..5343)
                     /locus_tag="VGP27_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7851485.1"
                     /translation="MTLPVLGPMTLTGFEHVWFFLFLFVVAGLVGLYIAMQAARRKRL
                     LRFANTELLESVAPKRPSRWRHLATVLLVLSLVMLTVAMAGPTHDVRIPRNRAVVMLV
                     IDVSQSMRATDVAPSRLAATQEAAKQFVDQMTPGINLGLISYAGAATVLVSPTTNRDA
                     SKNAIDKLQVADRTATGEAIFTALQAISTVGAVIGGGDTPPPARIVLMSDGKETVPSN
                     PDNPKGAFTAARTAKDQGVPIATVSFGTPYGTVDMNGQKTPVPVDDQMLKKVAQLSGG
                     QAYTASSVEQLKDVFTNLQEQIGYETQKGDAGTGWLRLGALVLALAAVAALLINRRLP
                     N"
     gene            complement(5366..6373)
                     /locus_tag="VGP27_09005"
     CDS             complement(5366..6373)
                     /locus_tag="VGP27_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7851486.1"
                     /translation="MTLPLLGPMTLSGFEHAWFLLFLLVALGIVALYIVVQFARQRRM
                     LRFANMELLESVAPKRPTRWRHLPAILLVLSLLLLTVAMAGPTNDVRIPRNRAVVMLV
                     IDVSQSMRATDVSPNRLAAAQEASKQFADELTPGINLGLIAFAGTATVLVSPTTNREA
                     TKNAIDKLQLADRTATGEGIFTALQAIATVGAVLGGGDQPPPARVVLFSDGKETVPSN
                     PDNPKGAFTAARTAKDQGVPISTISFGTPYGYVEINDQRQPVPVDDEMLRKIAELSGG
                     EAYTASSLDQLREVYANLQQQIGYETIKGDASAGWLRLGALILALSALAAMLLNRRLP
                     N"
     gene            complement(6397..7344)
                     /locus_tag="VGP27_09010"
     CDS             complement(6397..7344)
                     /locus_tag="VGP27_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7851487.1"
                     /translation="MTTSGRNVDLPSLKRGEIRDPALTAALRKLELTVRRKLDGVLHG
                     DHLGLLPGPGSEPGESRIYQPGDDVRRMDWSVTARTTHPHVRQMIADRELETWLVVDM
                     SASLDFGTTGCEKRDLAVAAAAAITFLNSGGGNRIGAIIANGDAVRRVPALSGRMHEQ
                     EMLRAIATMPKAPTGVRGDLAAAIDALRRPERRRGMAVLISDFLGPINWMRPMRAIAG
                     RHEVLGIEIIDPRDVELPPVGDVILQDTESGVTREFTIDEQLRSDFERAAAAHRAEVA
                     RTLRRCDAPLLTLRTDRDWIADVVRFVANRRRGALAGRA"
     gene            complement(7341..8462)
                     /locus_tag="VGP27_09015"
     CDS             complement(7341..8462)
                     /locus_tag="VGP27_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7851488.1"
                     /translation="MTSAGGPQGPAQGYPANTQGGPPPPANPGGLQSEVHTLERAIFE
                     VKRIIVGQDLLVERMLVGLLAKGHVLLEGVPGVAKTLAVETFAKVVGGTFARIQFTPD
                     LVPTDIVGTRIYRQGKEEFDIELGPVVCNFLLADEINRAPAKVQSALLEVMAERKISI
                     GGKTFPMPNPFLVMATQNPIEQEGVYALPEAQRDRFLFKLNIDYPTPEEEREIVYRMG
                     VKPPEPKQILAPGDLVRLQDAAANTFVHHALVDYVVRVVTATRTPEKFGMPDAKAWIA
                     YGASPRASLGIIAASRALALVRGRDYVVPQDVVEVIPDVLRHRLVLTYDALADEISSE
                     TVINRILQTVALPQVNAIPQQGHSVPPVVPAAAAAASGR"
     gene            complement(8599..>9043)
                     /locus_tag="VGP27_09020"
     CDS             complement(8599..>9043)
                     /locus_tag="VGP27_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEV7851489.1"
                     /translation="SSQGAVMPGGRVRGPQAIEYAIRRGGSQMGVPYSWGGGTLTGPG
                     PGVDDDAGKIGFDCSGLTRYMFAGVGVQIPKYSGDQYDTGRKVPVAQAKRGDLIFWGP
                     GGSQHVAMYLGGGRMLEASGSAGKVTVSPVRTSGLQPYAARIIES"
BASE COUNT         1478 a         3036 c         3009 g         1520 t
ORIGIN      
        1 gacacgacgc gctcgcgcag gtcgacgcgc gcacggatct tgtcgatgaa cggcacgagc
       61 agcgtcagct gccccgaaac ggtcttggtg tagcggccca gtcgttcgat caccgcggcc
      121 tcggcctgcg ggatgatcgc gatcgacttg gcgacgatga tggccgcgaa caccaccaac
      181 acccccacga ggaccaggcc ggcgacggca ccttccatga cggttccttc cgtagttgcc
      241 caggcttctc tagacgactt tttgaactac cgcggtggcc ccgtcgatgt gtacgacggt
      301 cacgtggtcg cccggttcgt agacatcgtc gtcgctcacc ggtcgcgccg tccatatttc
      361 accgtcgagc ttcacctggc cttcatggcg cgcaacccgg tccagcacca gcgcgctctt
      421 gccctcgagc tccgtcggcc ggtccggcag gcccgtgccc gacatgaaat gccgtcggag
      481 cgcgggtcgg accatcacca gcagcagcac cgacaccacg gcaaacaccg caccgtgaac
      541 ccacagctcg tcgaagacca ggctcgaccc cgcggccgcg agtgccccgc cgccgagcat
      601 gagcagaaac aggtcgccgg tgagcgcttc ggccccggcg agggccagcg ccgcgatcag
      661 ccagatcagc gcaatggaca tgccgaaagc ctagcgcgtg gtccgccccg cgagcggcga
      721 acaactacac tgcgagcatc atgtggtgcc ctagtgtttc gctgtcggtg tgggccaatg
      781 cctggcttgc cggtgccgcc gcgcccgatg acgttcttga tgcgctatcg caatgggcgc
      841 caaggcattc ggtcaccgcg tatgacgccg ttgccgccga aagcactggc ctgccctggc
      901 ccgatgtgaa ccactccgga acgatgtcac tgctgcagac gctgcggacc gcggcagggg
      961 cggtcaccgg cggacccgcg atgtccgtcg tcctccccgt gccgggcgac gtcagggggc
     1021 tgccggcggg gacccagttt cagcgcgacg cggtgacggc cggcgaggcc atcgtcatca
     1081 actcgggttc gtcgtcgcag acgatcggca tcgtgcccga tttcgagtac gacgacaccg
     1141 aaccctacga cgactcggat gacgccgagc tgtccggcct gaactggaca gtgtattcgc
     1201 ttcccgccgc gccgatccct gagcacatcg acctcggtga ggcggaattc gcactgcggt
     1261 cggcggttcg gtcggccgcc gacgctctgg gcgccatccg cgcagaggcc ggcggcatgg
     1321 acgtcgccga cccgcgcagg ctggtcgagc aggtgatgga gtcgacgggt cagcatcgcg
     1381 cacccgacca cgccccgtcg cgcgcattgc gggtgctgga gaacgccgcg cacatcgacg
     1441 ccatcgtcac ggtcagttcc ggcttgatgc ccatcggcct gcaaagctca tcggaggcgc
     1501 agagcgccaa cgaagcgctg cgaccgcttt ccgcggtggt gcgttcggcc cggctggcgg
     1561 ccgtcggcgc gatcctgcac tccgcctggg gctaggactt tccgcactca ggcgtgcaca
     1621 gtgcgccgtc gaccgagaag ccgtacagcg gagggggttc gacgcctggc ggactttcgg
     1681 tgcgggacgg gtcgctgccc tcccgcagct cgtcgatcag gtcgatcgcg agccgcgcga
     1741 agcgacggtc ggcattgggt gtggccgcgc gggccaacgc caccccgagt tcgtccgcct
     1801 gccgacgcag ctcgttgtcc agatcccata ccacctcgat gtggtcggcg acgaaaccga
     1861 tcgggcagac gatgaccgcc ttggtgcccg cgtcggccag ggcggacaga tggtccccca
     1921 cgtcgggttc cagccacggc acctgtggcg gacccgaccg cgactgccac acctggtcgt
     1981 agtcggcgta gcccgcctcg gccgcgacga ggtgcgatgc gtaggccacc tggcgggcgt
     2041 aaaggtcgtc gccacagcgg gatcgggccg caaacggaat ggagtgggcg gtgaacacca
     2101 gccgggcgtc cgcgcggagt tcggcgggca gcgcctgtgc cgcgtcgccg atagcctcgg
     2161 cgaagatctc gacgaacagc ggatggtcgt aatactggcg aagcttgacc agttggggag
     2221 cgcgctctcc ggccgcttgc cgaccacgag cgatgtcctc gttgtactgg gtgcaactgg
     2281 aatagccgcc ccatgccgat gtcgtgaaga cagccgcgcg acgaacgccg ttgtcgcgca
     2341 tcgcggtaac cgcatcctcg acatagggtt cccagttgcg gttcccgaag tacaccggca
     2401 gcgccaggcc gcggtcggcc agttcggcct ccagttgctc gatcaacgcg cggtttatgc
     2461 cgttgatcgg tgaggcacca ccgaaatgta ggtagtgctc ggcgaccgcg gagagccgct
     2521 cccgcgggat gccgcgcccg cgagtgacgt tctccaggaa cggcatcaca tgctcgggag
     2581 cctccggccc gccgaaggac agcagcagga gggcgtcgaa gtcggagctg tccattcttt
     2641 agagcagttg cgtgtgcgcg ccgccgtcgg cgtagacgac ggtaccggtg gtggcgggca
     2701 gccaatcgga gagcagggcg cacaccgtcc tggcgaccgg cgtgggatcc ttcatgttcc
     2761 aaccgacagg agcgcgctgg tcccagccct cctcgagtag ccggatctgg tcgccggcat
     2821 cggcaccgag cgcaccgccg acgatggcac tcatcgccag cgtccggatc gggcccgccg
     2881 caacgagatt cgaccgcacg ccgaactgac ccgcttcacg cgcgacgaac cggttcaccg
     2941 actcgagcgc gctcttggcg acggtcatcc agttgtaggc gggcatggcc ctcgtcgggt
     3001 cgaagtccat gccgacgatc gagccgcccg cgttcatgat cggcagcgtc gccttggcca
     3061 gcgatgcata cgagaaggcc gagatgtgga tgcccttgga gacgtcctcg tacggcgcgt
     3121 cgaagaacgg gttgatgccc ataccggact gcggcatgaa gccgatcgag tgcacgacac
     3181 cgtcgagttt gttgtccgca ccaatgacct cggtcacccg gtcggccagc gtcgcgagat
     3241 gctcctcgtt ctgcacgtcg agttccagca gcggcgcctt ctccggcagc cggtcggcga
     3301 tgcggccgat cagcttcatc cggtcgaagc ctgtgagcac caattgggcg ccggattcct
     3361 gggccaccct ggcgatgtgg aacgcgatcg acgagtcggt gatgatcccc gtgacgagga
     3421 tgcgcttccc ttcgagcaga cctgccattg tgaatgtcct tttctctatc gaagaatcag
     3481 tggcccatgc ccatgccgcc gtcgaccggg atcacggcac cggatatgta gctggcatct
     3541 tcggacgcca gaaagctgac cactccggcg acctcggcgg cggtgccaac ccgcttggcc
     3601 ggaacgaacc ccaatgcccc ctcctgaatc cgctcgtcca gcgcgcgggt catgtcggtg
     3661 tcgatgtagc ccggggccac gacgttcgcg gtgatcccgg ccttcgtcag ctcccgggcg
     3721 atcgagcggg ccatgccgat caggccggcc ttggaagccg cgtaattggc ctggttgccg
     3781 acgccccacg tgccggagac cgaacccacg aagatcatcc ggccgaaccg ctttcgcagc
     3841 atgctgcgcg acgcgcgctg ggccacccgg aacgccccgg tcaggttcgc gtcgatgacc
     3901 ttctcgaact tctcctcggt catccgcatg aggaacgcgt cggcggccac gccggcgttg
     3961 gcgatcacca cctcaaccgg accctggctt tcctcgactt cggtgaaggc ccggtcgaca
     4021 gctgcggagt cggtgatatc gcactcgacg ccgaacaacc cgtcgggcac cccggacccg
     4081 cggtgcgtgg cggccacctt gtggccgtcg gctgcgagcc gcagggcgat cgcgaggccg
     4141 atgccgcggt ttccgcccgt gaccagcacc gaacgcgaaa cgaaaggcgg acgaccgcca
     4201 gccttttggt cggcagtctc cggtactgca ctgtgggtca tgcccgccaa cttaacgtgt
     4261 gtcgaccatt cctaagaaat cgccttacga gggttgggcc cacgcccgta gggcagggga
     4321 cgactgggga ttgttctaat tcggcagcct gcgattgatc agcagcgccg cgacggccgc
     4381 cagcgcaagc accaaggcgc cgagccgcag ccagccggtg cccgcatcgc ccttttgtgt
     4441 ctcgtagccg atctgctcct gcagattcgt gaagacgtct ttgagttgct cgacgctcga
     4501 tgcggtgtag gcctgtccgc cagacagctg agcgaccttc ttgagcatct ggtcgtcgac
     4561 gggtacgggc gttttctggc cgttcatgtc aacggtgccg tacggcgtgc cgaacgacac
     4621 ggtcgcgatg ggcaccccct gatccttcgc ggtacgggcg gcggtgaagg cgccttttgg
     4681 attatccgga ttcgacggca cggtttcctt gccgtcggac atcaggacga tgcgtgccgg
     4741 cggcggcgtg tcacccccac cgatcaccgc accgacggtg gagatggcct gcaatgcggt
     4801 gaagatggcc tcgccggtcg cggttcggtc ggccacctgc agcttgtcga tcgcattctt
     4861 ggatgcgtca cgattggtgg tcggcgacac cagcacggtg gccgcccccg cgtaggagat
     4921 cagcccgagg ttgatgcccg gcgtcatctg atcgacgaac tgcttggcgg cctcctgcgt
     4981 ggcagccagc cggctgggtg ccacgtccgt ggccctcatc gactgcgata cgtcgatcac
     5041 caacatgacc accgcgcggt tgcgtggaat gcggacgtca tgggttggcc cggccatcgc
     5101 gaccgtgagc atcaccagcg acaacaccaa cagaaccgtc gccagatgcc gccaccgtga
     5161 cggccgtttg ggcgcgacgc tttccaacaa ctccgtgttc gcgaaccgca gcaaccgctt
     5221 gcgccgggca gcttgcatgg cgatgtagag ccccaccagt cccgccacga caaagaggaa
     5281 cagaaagaac cagacgtgtt cgaagcccgt cagcgtcatc ggtccgagca ctggtaacgt
     5341 catgcgctgc ttgccatttc cgttgctagt tgggtagtcg gcggttgagc agcattgctg
     5401 ccagcgccga aagggccagg atcaaggcgc cgagtcgcag ccagcctgca ctggcgtcac
     5461 ccttgatcgt ctcgtagccg atctgctgct gcaggttcgc gtagacctcc cgcagctggt
     5521 cgaggctcga tgccgtgtat gcctcaccgc cggacagttc ggcgatcttg cgcagcatct
     5581 cgtcgtcgac aggcaccggc tggcgctggt cgttgatctc gacatagccg tacggtgtgc
     5641 cgaacgagat ggtcgagatc ggcacaccct gatccttcgc ggtgcgcgcg gcggtgaatg
     5701 cgcccttcgg gttgtccgga ttcgacggca ccgtctcctt gccgtcggag aacagcacga
     5761 ctcgggcggg cgggggctgg tcgccaccac cgagcacggc gccgacggtg gcgatggcct
     5821 gcagggcggt gaagatgcct tcgccagtcg cggtgcggtc ggctagctgc agcttgtcga
     5881 tcgcattctt ggtcgcttcg cggttggtgg tcggcgacac cagcacggtg gcagtacccg
     5941 cgaaggcgat caggccgagg ttgatgcccg gcgtcagctc atcggcgaac tgcttggatg
     6001 cctcctgcgc ggctgcaaga cgattcggcg acacatcggt agcgcgcatg gattgcgaca
     6061 cgtcgatcac caacatgacg acggcgcggt tgcgtgggat ccggacgtcg ttcgtcggcc
     6121 cggccatcgc aacggtgagg agcaatagtg agagcaccaa caggatggcc ggcaggtgcc
     6181 gccagcgagt aggcctcttg ggcgcaacgc tttccagcag ctccatgttc gcaaaacgca
     6241 gcatccgtcg ttgacgggca aattgaacga cgatgtagag cgcgacaatt ccgagagcaa
     6301 cgagaagaaa caggaggaac caagcgtgtt cgaagcccga cagcgtcatc gggccgagca
     6361 acggtaacgt catgtggcag ttgtcatttc gttggactat gcgcggccgg cgagcgctcc
     6421 acggcgacgg ttcgcgacga accggaccac gtcggcgatc cagtcccggt cggtgcgcag
     6481 tgtcagcagc ggcgcgtcac agcgccgcag ggttctggcc acctcggcgc ggtgcgcggc
     6541 ggcggccctc tcgaagtcgg agcgcagctg ctcatcgatg gtgaattcgc gggtgacacc
     6601 ggactcggtg tcctgcagga tcacatcgcc gacgggcggc agctcgacgt cgcgcgggtc
     6661 gatgatctcg atgccgagca cctcgtgccg tccggcgatg gcgcgcattg ggcgcatcca
     6721 gttgatcggg ccgaggaagt cactgatgag caccgccatg ccgcgacggc gctcgggcct
     6781 gcgcagcgca tctatcgccg cggccagatc gccccgcacg ccggtcggcg ccttgggcat
     6841 ggtggcgatc gcgcgcagca tctcctgctc gtgcatgcgc cccgagagcg cgggcacccg
     6901 gcgcacggcg tcgccgttgg cgatgatcgc gccgattcgg ttacctccgc cgctgttgag
     6961 gaacgtgatc gccgccgctg cggcaaccgc gagatcgcgc ttctcgcagc cggtggtgcc
     7021 gaagtcgagg ctggccgaca tgtcgaccac caaccacgtt tccaactcgc ggtcggcgat
     7081 catctggcgg acgtggggat gcgtcgtgcg cgcggtcacc gaccagtcca tccgccgcac
     7141 atcgtcgccc ggctggtaga tgcgcgactc ccccggctcc gatccgggac cgggcagcag
     7201 gccgaggtgg tcgccgtgca gcactccgtc gagcttgcgg cgcaccgtca gttcgagttt
     7261 gcgcagcgct gccgtgagcg cggggtcacg gatctccccg cgcttcagcg acggcagatc
     7321 gacgttgcgt ccggaagtag tcaccgaccg ctagccgcgg cagctgcggc gggcacgacg
     7381 ggtggcaccg aatgaccttg ctgcggaatg gcattcacct gcggcagggc gacggtctgc
     7441 aggatccggt tgatcaccgt ctccgacgag atctcgtcgg cgagcgcgtc gtaggtcagc
     7501 accagccggt gtcgcagtac gtcgggaatg acctctacga cgtcttgggg gacgacgtag
     7561 tcgcggccgc gcaccagcgc cagcgccctc gaagcggcga tgatgccgag cgaggcgcgt
     7621 ggcgaggcgc cataggcgat ccacgccttg gcgtcgggca tgccgaactt ctccggtgta
     7681 cgggtcgccg tgacgacccg cacgacatag tcgacgagcg cgtggtgcac gaacgtgttg
     7741 gcggccgcgt cctgcagccg gaccaggtca ccgggagcca ggatctgctt gggctccggc
     7801 ggcttcacgc ccatccggta gacgatctca cgctcttcct ccggcgtcgg gtagtcgatg
     7861 ttcagcttga aaaggaagcg gtcgcgctga gcctcgggaa gcgcgtacac gccttcctgc
     7921 tcgatcgggt tctgggtcgc catcaccagg aacgggttcg gcatcgggaa cgtcttgccg
     7981 ccgatcgaga tcttgcgctc ggccatcacc tccagcagcg ccgactgcac cttggccggg
     8041 gcgcggttga tctcgtcggc aagcaggaag ttgcagacca ccgggccgag ttcgatgtcg
     8101 aactcctcct tgccctgccg gtagatccgg gtgccgacga tgtcggtggg caccaggtcg
     8161 ggcgtgaact ggatgcgcgc gaacgtgccg ccgaccacct tggcgaacgt ctccacagcc
     8221 agcgtcttgg cgacgccggg caccccctcg agcagcacgt gccccttggc gagcaggccg
     8281 acgagcatgc gttcgaccag caggtcctgg cccacgatga tgcgcttgac ctcgaagatg
     8341 gcacgttcca gggtgtgcac ctcggactgc aggccccccg gattggcggg cggcggtggg
     8401 ccaccttgag tgttggcggg ataaccctgc gccgggcctt gcggtccacc tgctgacgtc
     8461 atcgacgatc ctcttacgtt gtatctggta aagacgcagg tcgtgcgccg cggacggggt
     8521 gcccgccgtc aactattcca ggcactacgg aatccgtcga cgttgcccgg cactgctcag
     8581 ggtccgctca ggccggtctc aggattcgat gatgcgggcg gcgtacggct gcaatcccga
     8641 ggtgcgcacc gggctcaccg tcaccttgcc cgcgctgccc gacgcctcga gcattcgccc
     8701 gccgccgagg tacatggcga cgtgctggct gccgcccggt ccccagaaga tgaggtcgcc
     8761 gcgcttggcc tgggccaccg gcaccttgcg tccggtgtcg tactggtcac ccgaatactt
     8821 cgggatctgc acaccgacgc cggcgaacat gtagcgggtg agccccgagc agtcgaaacc
     8881 gatttttccg gcgtcgtcgt cgacgccggg cccggggccg gtgagcgtgc cgcctcccca
     8941 tgaatacggc acgcccatct gcgaaccgcc gcgtcgaatc gcatactcga tcgcttgcgg
     9001 cccgcgaacc cggcctccgg gcatgacggc accctggctg ctg
//
LOCUS       DASYLX010000366         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1553292,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000366 DASYLX010000000
VERSION     DASYLX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553292"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VGP27_09025"
     CDS             <1..129
                     /locus_tag="VGP27_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892620.1"
                     /note="forms citrate from oxaloacetate and acetyl-CoA;
                     functions in TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV7851490.1"
                     /translation="GWSAHILEQKRLGKLVRPSAIYVGPAPRSPESVEGWNEIAKG"
     gene            126..752
                     /locus_tag="VGP27_09030"
     CDS             126..752
                     /locus_tag="VGP27_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7851491.1"
                     /translation="MTTVAATVTAFESAARSFAGLVHRIPADGWDGPGLGEWDMRSLV
                     GHASRSLITVSTYLETSAEHEDVATPQDYYAGIADYMSSTNAEAITERGRQAGRDLGD
                     DPAQTIDDLVERVLGELAPAAADPLVAVIGGLGIRLHTYLPTRTFELAVHSLDIARAA
                     ALSFDLPDIVLDQATVLAARIAVTTGRGSEVLMSLTGRRVLPPGFSVV"
     gene            816..1265
                     /locus_tag="VGP27_09035"
     CDS             816..1265
                     /locus_tag="VGP27_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851492.1"
                     /translation="MAIEVQPAVLPHLIVDDAAAAIDFYVKAFGAVEVGRVPGPDGKL
                     VHAALAINGSTVMLNDDFPEMSGGKSMTPKALGGSPVTIHLVVTDVDAKFQQAIDAGA
                     SVVMPVDDQFWGDRYGVLRDPFGHQWSMGQPVREVSMDEIEEAMKRQ"
     gene            complement(1281..1460)
                     /locus_tag="VGP27_09040"
     CDS             complement(1281..1460)
                     /locus_tag="VGP27_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEV7851493.1"
                     /translation="MPNPIDLQKSLGGVNYPARKDDLVEHARKHGAKDEIVESLKGIP
                     DKEYDGPDQVQKEVF"
     gene            complement(1623..>2898)
                     /locus_tag="VGP27_09045"
     CDS             complement(1623..>2898)
                     /locus_tag="VGP27_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7851494.1"
                     /translation="VFERLPTPYGLVRAGVAPDHQSTKRVTRLFDKIAKRRGFRFFLN
                     VEVGSDLSHAEVLDHHHAVLYAVGAPNDRRLEIDGMGLPGTGTATEMVAWVNGHPEYT
                     DLPVDLGCERVVIVGNGNVALDVARILTTDPDGLSHTDISDQALAALRQSKVTEVVIA
                     ARRGPAQSAFTLPELVGLTSTCDVVLDAGPEVDVHDLVLADLATVEDPLTRNKLEILR
                     KLGDASVPATRPRIRLAYRMTPHRVLGADRVTGIEFTVTGTDETRRLDSGLVLTSIGY
                     RGRPIRDLPFDEAAAVVPNDGGRVVDPRTGRPVRGSYVAGWIKRGPTGFIGTNKSCAF
                     QTVQNLVDDYNAGLLTDPVAKPAELDKLVRSRRADVVDAAGWHAIDEAEIARGGENRP
                     RNKFTSIADMLAVAATAPAPPLRQRLLAGLRR"
BASE COUNT          460 a          959 c          984 g          495 t
ORIGIN      
        1 ggttggagcg cgcacatcct cgaacagaag aggctcggca agctcgtccg cccgtcggca
       61 atctacgtcg gccccgctcc gcgcagcccc gaatccgtcg agggctggaa cgagatcgcg
      121 aaaggctgac cacggtggcg gccacggtca ccgctttcga gtcggcggca cgaagcttcg
      181 ccggcctggt ccaccggatc cctgccgacg gctgggacgg gccgggactc ggcgagtggg
      241 acatgcggtc gttggtcggg cacgcgtcgc gttcgctcat caccgtcagc acgtatctgg
      301 agacgtccgc ggagcacgag gacgtcgcga ctccgcagga ctactacgcg gggattgccg
      361 actacatgtc gagcaccaat gcggaggcga tcaccgaacg cggcagacag gcgggccgcg
      421 atctcggcga cgaccccgca cagacgatcg acgacctggt cgaacgcgtg ctgggcgaac
      481 tcgcgccggc ggccgccgac ccactcgtcg cggtgatcgg cgggctgggg atccggctgc
      541 acacctatct gccgacgcga accttcgaac tggccgtgca cagcctcgat atcgcacgcg
      601 cggccgcgct gtctttcgat ctgcccgaca tcgtgctcga ccaggccacg gtgctggcgg
      661 cgcggatcgc cgtgacgacc gggcggggca gcgaagtgct gatgtcgctg acagggcgac
      721 gtgtccttcc gccgggattt tcggtcgtat gaccgcgatg aattttcggc gcggcgatag
      781 tcgttagtag cgaccacccc agacaggaga ccaccatggc aatcgaagtc cagcccgcgg
      841 tgctgccgca cctgatcgtc gacgacgccg ccgccgcgat cgacttttat gtgaaggcgt
      901 tcggcgccgt cgaagtcggc cgggtgcccg gtccggacgg caagctggtc cacgccgccc
      961 tcgcgatcaa cggctctacg gtgatgctga acgacgactt cccggagatg agcggcggta
     1021 agtcgatgac accgaaagcg ctcggcggtt caccggtgac catccacctc gtcgtcaccg
     1081 acgtggatgc gaagttccag caggctatcg acgcgggcgc gagcgtcgtc atgccggtcg
     1141 atgaccagtt ctggggcgac cgctacggcg tgctgcgcga cccgttcggc caccagtggt
     1201 caatgggcca gccggtgcgc gaggtcagca tggacgaaat cgaggaggcg atgaagcggc
     1261 agtagccgct gcacccgcgg ctagaagacc tctttctgga cctggtcggg gccgtcgtac
     1321 tccttgtcgg gtatcccctt gaggctctcg acgatctcgt ccttggcccc gtgcttcctg
     1381 gcatgctcca cgaggtcgtc cttgcgcgcc gggtagttga ctccgcccag tgacttctgc
     1441 aggtcgatgg gattaggcat tcggttgctc ctttgcgact ggctatcgcg ccggggtagc
     1501 cgaatgcctt cgagtgaaac ctcccccgtc acagcgtgtg acgtttcgca gaaaatgttc
     1561 gagaagtgct ctgcgaaacg ttgattgctg cgattccagt gtcgaacggt gccggtactg
     1621 cgtcagcgcc gcagcccggc cagcaggcgc tgccggagtg gaggagcggg tgcggtggcc
     1681 gccacggcca gcatgtcggc gatcgacgtg aacttgttcc gtggccggtt ttctccgccg
     1741 cgggcgatct cggcttcatc gatggcgtgc cagccggccg cgtccaccac gtccgcccgg
     1801 cggctgcgca ccagcttgtc caactccgcc ggtttggcca ccgggtcggt gagcaggccc
     1861 gcgttgtagt cgtcgaccag gttctgcacc gtctgaaacg cgcacgactt gttggtgccg
     1921 atgaagccgg tcggtccgcg tttgatccaa cccgcgacgt agcttccgcg caccggtcgg
     1981 ccggtccgcg ggtcaacgac ccggccgccg tcgttcggca ccaccgcggc ggcttcgtcg
     2041 aacggcagat cccgaatcgg cctgccgcgg tagccgatag acgtcagcac gaggccggag
     2101 tcgagccgac gggtttcgtc ggtaccggtc accgtgaact cgatgccggt gacccggtcg
     2161 gcgccgagca cccggtgcgg cgtcatgcgg taggcgagac ggatccgcgg gcgcgtcgcg
     2221 ggtaccgacg cgtcgccgag cttgcgcaga atctccaatt tgttgcgggt cagcgggtcc
     2281 tcaacggtgg ccaggtcggc cagcaccagg tcgtggacat cgacttctgg gccggcgtcg
     2341 agcaccacgt cgcacgtcga cgtcagcccg acgagttcgg gcagcgtgaa cgcggactgc
     2401 gcgggcccgc ggcgggcggc gatcaccacc tcggtgacct tcgactgccg cagcgccgcc
     2461 agtgcctgat cggagatgtc ggtgtggctc aacccatccg ggtcggtcgt caagatacgt
     2521 gcaacatcca gggcgacgtt tccgttgccg acgatgacca cgcgctcgca ccccagatcc
     2581 accggcaggt cggtgtattc gggatgtccg ttgacccatg cgaccatctc ggtggccgtg
     2641 cccgtgccgg gcagccccat gccgtcgatc tcgagcctgc gatcgttcgg ggcgccgacg
     2701 gcgtagagca ccgcgtgatg gtggtccagc acttcggcgt gggacaggtc cgaacccacc
     2761 tcgacattga ggaaaaaccg gaagccccgt cgcttggcga tcttgtcgaa cagccgggtg
     2821 accctcttgg tgctctggtg gtcgggcgcc acgcccgcac gcaccaggcc atacggagtt
     2881 ggcagccgtt cgaagacg
//
LOCUS       DASYLX010000367         4135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3527094,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000367 DASYLX010000000
VERSION     DASYLX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527094"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGP27_09050"
     CDS             <1..822
                     /locus_tag="VGP27_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEV7851495.1"
                     /translation="YAGAQLALAEAYRNVAVTGATPIAVTNCLNFGSPEDPGVMWQFS
                     EAVRGLADGCAALGIPVTGGNVSFYNQTGTTAILPTPVVGVLGVLDDVRRRIPTGLGT
                     EPGEALLLLGDTRDEFDGSIWAQVSADHLGGRPPEVDLAREQLLAEVLSAASRDGLVS
                     AAHDLSEGGLIQAVVEAALTGETGCRIVIPEEWQEGTGPFTFLFSESAGRVLVAVPRT
                     EESRFRSMCEARGLPVTRIGVVDQGSDALEVQDQFTVSLEDLRSTSEGVLPGLFG"
     gene            912..2585
                     /locus_tag="VGP27_09055"
     CDS             912..2585
                     /locus_tag="VGP27_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851496.1"
                     /translation="MVRLDFVGIAFGALFFCLSLTPSLLPRDWLFQGLIGGVNAAIGY
                     GIGVFVGKMLRRFVLRRRSWWPPRKRVLYWFKAATITLATTASLLMLIPAAAWQRRVS
                     AEMGMEGPGTVGYLRTLIIAMIVAGLCVAAARITIDLIKTMARFFIRRWHLHDEVALF
                     IGTAIVVVLAITLINGVLIRGFLAVSNRVFQPQNTTTRQGITQPLELERSGSPTSFAP
                     WDSLGYQGRNFVATGPHAFELTRLNGAPAKEPIRLYAGLQTAGTDDGRAAILVRELDR
                     TRAFDRKVLAIVPTTGTGWVNPVAARALESMYNGDTAMVASQYSYLPSWISFLGDRDK
                     SMESGRTMIEAVHAWWLRLPPDRRPKLLLYGESLGSMAGQGAFDWLPDIARMGFSSVL
                     WVGPPNASPLWKAITVRRDPGTPEVEPRYDNGRTVRFSQANDAADIAADTDAPWEGTR
                     VLFLQHASDPIVWWSGDLIFNRPDWLKEPPGRDRTPLMRWYPIVTFWQVAADMTNAGS
                     VPGGHGHNYGEYVLDGWAAAAPPDGWTPDDTERIRIALEKTDANEGPEY"
     gene            2585..3214
                     /locus_tag="VGP27_09060"
     CDS             2585..3214
                     /locus_tag="VGP27_09060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV7851497.1"
                     /translation="MRTKAFRAVTVAAGVVGWSGVIDPRLPPRVQPVARAALGAVLVA
                     ATRAPLGLRPPQLWSGLRLGFAVAAPVILGVAASTAAPAVRSAMADRDLPDSASGWLA
                     VRIPFGTVWSEEAAYRAALGTMAAGAFGPTAGRLLQSVAFGLSHIADARGTDEPVIAT
                     VLVTAVAGWFFGLLLERSGSLAAPMLAHLAINEAGAVAAVAVQRRSATG"
     gene            complement(3232..3357)
                     /locus_tag="VGP27_09065"
                     /pseudo
     CDS             complement(3232..3357)
                     /locus_tag="VGP27_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MCE-family protein MCE1A"
     gene            3381..3773
                     /locus_tag="VGP27_09070"
     CDS             3381..3773
                     /locus_tag="VGP27_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HEV7851498.1"
                     /translation="MPARRTADPEKTRAAVLAVAEWLRDENRPAPQRSLLAEAVRLTA
                     RTIAATAPGASVEVRVPPFVAVQCISGPRHTRGNPPNVVEADPRTWLLLATGLRTVAD
                     AVSDGELQLSGARAPEIATLLPVVPLDG"
     gene            3924..>4135
                     /locus_tag="VGP27_09075"
     CDS             3924..>4135
                     /locus_tag="VGP27_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007630685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851499.1"
                     /translation="MTVEQTDSTDTLPASSPRQAGAPVSDEPKEECGVFGVWAPGEEV
                     AKLTYYGLYALQHRGQEAAGIAVADGS"
BASE COUNT          639 a         1428 c         1385 g          683 t
ORIGIN      
        1 tacgcgggcg cgcaactcgc gctggccgag gcataccgca acgtcgcggt gaccggcgcc
       61 accccgatcg cggtcaccaa ctgcctgaac ttcggttcgc cggaggatcc aggtgtcatg
      121 tggcaattct ccgaagccgt ccgcgggctt gcggatggct gtgcggccct tggcattccg
      181 gtcaccggcg gcaacgtgag cttctacaac cagaccggca cgacggccat tctgcccacc
      241 ccggtcgtcg gggtgctggg tgtgctcgac gatgtccgcc gtcgtatccc gacggggctt
      301 ggcaccgaac ccggagaggc cctgctgctg ctcggcgaca cccgcgacga gttcgacggc
      361 tccatctggg cgcaggtcag cgccgaccat ctcggcggcc gccccccgga ggtcgacctg
      421 gcacgcgagc agttgctcgc cgaggtgctc agcgcggcgt cgcgggacgg actcgtctcg
      481 gccgctcacg acctgtccga gggcggcctg atccaggctg tcgtggaagc ggcgctgacc
      541 ggtgaaaccg gttgtcgcat cgtcattccc gaagagtggc aagagggcac aggcccgttc
      601 acatttctct tctcggaatc cgcgggcagg gtgctggtcg ccgtcccgcg caccgaggag
      661 agccggttcc ggtccatgtg tgaggcccgc ggcctgccgg tcacccgcat cggtgtggtg
      721 gatcagggga gtgacgctct cgaggtccag gaccagttca cggtgtcact tgaggacctg
      781 cgcagcacgt cggagggcgt gctgcccggt ttgttcgggt gagtgtcccg accaacgaca
      841 tcaccggcgc accggcttct gcaaaccacg agcgcaaccg ccgccacccc ctcctggtag
      901 gggggtggag tctggtgcgc ctcgacttcg tcgggatcgc gttcggcgcg ctgtttttct
      961 gtctgtcgct gacgccgtcg ctgctgccgc gcgactggct gttccaggga ctgatcggcg
     1021 gcgtcaacgc cgccatcggc tacggaatcg gtgtgttcgt cggaaaaatg ttgcggcgct
     1081 tcgtgttgcg acgcagaagt tggtggccgc cgcggaagcg ggtgctctac tggttcaagg
     1141 ccgccaccat cacgctggcg acaaccgcaa gcctgctgat gctgataccg gcggcggcct
     1201 ggcagcgacg ggtgtccgcg gaaatgggca tggaggggcc gggcaccgtc ggctacctgc
     1261 gcaccctgat catcgcgatg atcgtcgcag gcctatgcgt cgccgcagca cggatcacca
     1321 tcgacctcat caagacaatg gcccggtttt tcatccgtcg ctggcatctt cacgatgagg
     1381 tcgcgctctt catcggcacc gcgatcgtcg tcgtgctggc gatcacgttg atcaacggcg
     1441 tgctgatccg cggcttcctg gccgtctcga accgggtctt ccagccgcag aacaccacca
     1501 cacgacaggg catcacccag ccgctggagc tcgaacgatc gggcagccca acgtcattcg
     1561 ccccatggga ctcgttgggc tatcagggcc gcaacttcgt cgccaccggc ccgcacgcat
     1621 tcgagctgac ccgtctcaac ggcgcgcccg ccaaagaacc catccggctg tatgcgggtt
     1681 tgcagaccgc gggcaccgat gacgggcggg ccgccatcct ggtgcgcgag ttggatcgca
     1741 cccgcgcatt cgaccgcaaa gtgctcgcca tcgtcccgac cacgggtacc ggctgggtca
     1801 atcccgtcgc ggccagggcg ctggagtcga tgtacaacgg cgacaccgcg atggtcgcct
     1861 cgcagtactc gtatctgccc agctggatct cgtttctcgg cgaccgggac aagtccatgg
     1921 agtcgggccg gaccatgatc gaggcggtcc acgcgtggtg gctacggctg ccgcccgacc
     1981 ggcggccgaa gctgctgctc tacggcgaga gcctcggatc catggccgga cagggcgcat
     2041 tcgactggct ccccgacatc gcacggatgg gattctcttc ggtgctgtgg gttggcccac
     2101 ccaatgccag cccgctgtgg aaggcgatca ccgtgcgccg cgacccgggc acccccgagg
     2161 tcgaaccccg ctacgacaac ggacgcaccg tgcggttctc ccaggccaac gatgccgccg
     2221 atatcgccgc cgacaccgac gccccatggg agggcacgcg tgtgctgttc ctgcagcacg
     2281 cctctgaccc catcgtgtgg tggtcggggg acctgatttt caaccggccg gactggctga
     2341 aggagccgcc cgggcgggac cggacaccgt tgatgcggtg gtatccgatc gtcacgttct
     2401 ggcaggtcgc cgccgacatg accaacgcgg gttcggttcc cggcggacac ggtcacaact
     2461 acggcgaata cgtgctcgac ggctgggccg ccgccgcgcc gcccgacggc tggactcccg
     2521 acgacaccga acgcatcagg atcgcgctgg agaagaccga cgccaatgag gggcccgagt
     2581 attagtgcgc accaaagcgt tcagggcggt gacagttgcg gctggggtgg tcggatggag
     2641 tggggtgatc gatccgcggc tgccgccgcg cgtgcaaccg gtcgcccgcg cggcgctggg
     2701 agctgtactc gttgcggcga cccgcgcacc tctgggcctg cgtccgccgc agctgtggtc
     2761 gggactgcgg ctcggcttcg ccgtcgccgc accggtcatc ctcggcgtcg cagcatcgac
     2821 agcggcgccg gccgttcggt cggcgatggc cgatcgcgac ctgcccgact cagcgtcggg
     2881 ctggctggcc gtgcgcatcc cgttcggcac cgtctggtcg gaggaggcgg cgtaccgcgc
     2941 cgcccttggc acgatggccg caggcgcctt cgggccgacg gccggccggc tgctgcagtc
     3001 agtggcgttc ggactgtcgc acatcgccga cgcccgcggc accgacgaac cggtgatcgc
     3061 caccgtgctg gtgaccgccg tcgcgggctg gttcttcggg cttctgctgg aacggtccgg
     3121 cagcctggcc gccccgatgc tcgcccacct cgcgatcaac gaagcgggcg cggtggcggc
     3181 ggtcgctgtg cagcgccgat ccgctactgg ttgatcgtcg tctcccccga aatggttgta
     3241 cggcgcaatg ctgtagccgg tgtccgtcac cagatacgga aacggccaca ggaacttggt
     3301 gaccttctgc caacagccgg gacgaccctc cggacctccg tgcgcgttca cccgcggcga
     3361 acaccttgag acactgtgga atgcccgcac gccgcaccgc cgatccggag aagacgcgtg
     3421 cagccgttct cgccgtcgcc gagtggttgc gcgacgagaa ccggcccgcg ccgcaacgat
     3481 ccctgctcgc cgaagcggtt cgactgaccg cacgcactat cgcggcgacc gcgcccggcg
     3541 caagcgtcga ggttcgggtg ccaccgttcg ttgcggtgca atgtatttcg gggcccaggc
     3601 acacccgtgg caatccaccg aacgtcgtgg aggccgaccc gcgcacctgg ctgctgttgg
     3661 caacgggcct tcgcacggtc gccgacgcgg tctccgacgg cgagttgcag ctatcgggcg
     3721 cccgcgctcc ggagatcgcg acgctgctgc ctgtcgtgcc gctcgacggc tgaccgcggt
     3781 cgcaagagcc gcgggccggg aatccagacc ggtccgtgct cgttggttcc tatgggcgac
     3841 atgagcgcca gcgattccat gattgacccc cgatgaccgt agactgaaca cgtcacccac
     3901 cgccccatgg gagcagccct atagtgaccg tcgagcaaac ggactccacc gatactcttc
     3961 ccgcaagcag tccccggcaa gccggagccc ccgtatcgga cgaaccaaaa gaagagtgcg
     4021 gcgtattcgg cgtctgggcg ccgggcgaag aggtcgccaa gctcacctat tacggcctgt
     4081 acgcactgca acaccgaggt caggaagccg caggtatcgc ggtcgccgac ggctc
//
LOCUS       DASYLX010000368         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1332657,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000368 DASYLX010000000
VERSION     DASYLX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332657"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGP27_09080"
     CDS             complement(<1..305)
                     /locus_tag="VGP27_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7851500.1"
                     /translation="MRMATFNILHGRSVHDGAVQLDRLEESVRRIDADVLALQEVDCD
                     QPRSAMADLTGLAAQAMGAVTHRFVAAIAGTPGATWMAATGREQPGTAAYGIALLSR"
     gene            330..875
                     /locus_tag="VGP27_09085"
     CDS             330..875
                     /locus_tag="VGP27_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV7851501.1"
                     /translation="MTNTHLREALKQGASALKAHGPDFALAGSYALWAFGGPEPVHDV
                     DFVVAEQDTEAAALTLEKAGFRIERTPEDWLFKACVEQEFVIDVLHQLNGTPVDADMI
                     ASAQELDVLAIRMRVLPPTKVLVEKLNSLNEHHCDFAALLPAVRAVREQVDWVRLRAD
                     TAENDFAVAFLVLAERLGVTA"
     gene            complement(1176..>1655)
                     /locus_tag="VGP27_09090"
     CDS             complement(1176..>1655)
                     /locus_tag="VGP27_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEV7851502.1"
                     /translation="YVDRAGNATERRLEPYQLVTTGKRWYLLAFDRDREDWRSLRLDR
                     MHDVQARATTFVARDAPDAAAYVRRSISASPYRYVARVRYSAPEELVARHFSPSSVTI
                     EPDGPDACIVTAGADDPERMVFYFATAGADFEVLEPREVAIAVQSVADRLRRAASSP"
BASE COUNT          271 a          536 c          553 g          295 t
ORIGIN      
        1 aaccgggaca gcagcgcgat gccgtaggcc gccgtgcccg gttgttctcg gcccgtcgcc
       61 gccatccacg tcgcccccgg cgttccggcg atggccgcga cgaaccggtg cgtcaccgcg
      121 cccatcgcct gagccgccag cccggtcagg tcggccatcg ccgaccgcgg ctgatcgcag
      181 tcgacttcct gcagggccag cacgtcggcg tcgatgcggc ggacggattc ctcgagccgg
      241 tcgagttgaa cggcgccgtc gtggacgctg cgtccgtgca ggatgttgaa ggtggccatt
      301 cgcatctgca tgggtactag gtacccaaca tgacgaacac ccacctgcgt gaggcgctga
      361 aacagggagc ttcggcgttg aaggcgcacg gtccggactt cgcgctggcc ggtagttacg
      421 cgctatgggc ctttggcggt cccgaaccgg tgcatgacgt ggacttcgtc gtcgcggaac
      481 aggacaccga ggcggcggcg ctaacgctgg aaaaggcggg tttccgcatc gagcgcacgc
      541 cggaggactg gctgttcaag gcgtgcgtcg agcaggagtt cgtgatcgac gtgctgcacc
      601 agctcaacgg aacgccggtg gacgctgaca tgatcgcctc ggcccaggag ctcgacgtgc
      661 tcgcgatccg gatgcgggtg cttccgccga cgaaggtgct cgtcgagaag ctgaactcgc
      721 tcaacgaaca tcactgcgat ttcgccgcgc tgctgcctgc cgttcgggcg gtgcgcgaac
      781 aggtcgactg ggtccggttg cgtgccgaca ccgccgaaaa cgacttcgcg gtggcgtttc
      841 tggtgctcgc cgaacggctg ggtgtcaccg cttgatgctg tgcagggtga gcatcgtcac
      901 acctctatca atgtcacata tgccgtttcg tcgccgctgc gttgagtgtg gttgggccgc
      961 agttcatttc gattgaggtt tcattacgca gatcacggcg tgccgcctgc accattggcc
     1021 gcgaagtgcg acaatggagg catcatgacg actgaacaca agacgtctgg cggctcacgc
     1081 acctggtccg acccgctctc ggtgggtttc gaggcgcacc gccgactgct gctccttcgc
     1141 ctgcgcctgc gcggctctgc ggccagacgc gcccgtcacg gactgctggc agcacgccgt
     1201 aaccggtcag caaccgactg aaccgcaata gccacttcgc gcggttcgag cacctcgaag
     1261 tcggcgccgg cggtcgcgaa atagaacacc atccgctccg ggtcgtctgc gcccgcagtg
     1321 acgatgcagg cgtcgggacc gtccggttcg atagtcaccg acgacggcga gaagtgtcgt
     1381 gccactagct cttccggagc ggaatagcga acgcgggcaa cgtatcggta cggcgatgcg
     1441 ctgatcgacc gtcggacgta tgccgcggcg tcgggcgcgt ccctggccac gaaggtggta
     1501 gctctcgctt ggacatcgtg catccggtcc agtcgcaggc tgcgccagtc ctcgcggtcg
     1561 cggtcgaacg ccaatagata ccagcgcttc cccgtcgtga cgagctgata gggctccagc
     1621 cgccgctcgg tggcgttgcc ggcgcgatcg acata
//
LOCUS       DASYLX010000369         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3418305,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000369 DASYLX010000000
VERSION     DASYLX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418305"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGP27_09095"
     CDS             <1..495
                     /locus_tag="VGP27_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7851503.1"
                     /translation="PIAAVQSEWSIWSRDVERNVVPAAAELGVGFVPYSPLGRGFLTG
                     TVRSAADLASNNDFRKTMPRFAEGALDANLVVVDVVTSVADALGATAAQVALAWLRHR
                     AETFGVASVPIPGTRRAARVEENLGSLWVTLSGDQLTALDAAADAVSGNRSRDLNWVS
                     AGRE"
     gene            complement(500..790)
                     /locus_tag="VGP27_09100"
     CDS             complement(500..790)
                     /locus_tag="VGP27_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851504.1"
                     /translation="MRQCDSITVLAYRDRASTIVAEAEPMLRLCETAGTRYRIGVETQ
                     PPSSSVPTNTTFADDGEAVMRTQLTAVAARLQQPLFDGFAVHHLRAWQTMPP"
     gene            complement(807..1109)
                     /locus_tag="VGP27_09105"
     CDS             complement(807..1109)
                     /locus_tag="VGP27_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851505.1"
                     /translation="MDADPAETVATWLWQAGADIPKIVEFVAAPRLREVYVAVPLSGV
                     DDNVAALTTALRANGIAVSCLGGDPTWTVQHDTASNWAFRATTDAVFDAVHLDVEP"
     gene            1179..2042
                     /locus_tag="VGP27_09110"
     CDS             1179..2042
                     /locus_tag="VGP27_09110"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV7851506.1"
                     /translation="MIEVTLLGTGSPIPDPNRAGPSTLVRAGGQTFLVDCGRGVQQRL
                     AAVGSGANALSALLLTHLHSDHIADLGDVIITRWVTTFDPDAPALPIIGPPGTAEVVD
                     ATLKAFGPDIGYRIAHHPDITGPPRVEVEEVTGGVVWGRVGVRIRVALTDHRPVAPTI
                     GFRIEHDDASVVLAGDTVPCAGLDDLAAGAGALVHTVIRKELISQMPQQRIRDICDYH
                     SSVEEAAATAERAGVGILVLTHYVPGIAPGQEDDWRALAATVFSRQIELGDDLHRVEV
                     HPGVCGGRGGN"
     gene            complement(2039..>2821)
                     /locus_tag="VGP27_09115"
     CDS             complement(2039..>2821)
                     /locus_tag="VGP27_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851507.1"
                     /translation="LTSGRVHAQRFGDPDAPLVLAVHGLSANMHGFDYLAPHLVADGG
                     RQVVAVDLRGRGGSDSTAPGTYGLASHARDMFEAATLLGHQQFDWVGWSLGALTGILA
                     AELEPGRIRRLGLIDHAGRSDEGAVAAVLAGLNRLDLDLSTAEGYVSQVEAVSPIRPF
                     TDFWRRFYAYEFRRTTKAACLEDGRDASGREWPHQWRHLTMPVGLVRCRQPLNGGLVV
                     PDDVAADIDSTVEALTITDVDADHFTVMTYEPAAEALRALIG"
BASE COUNT          439 a          926 c         1010 g          446 t
ORIGIN      
        1 ccgatcgccg cggtgcagag cgagtggtcg atctggagcc gtgacgtcga gcgcaatgtc
       61 gtgcccgcgg ccgccgagct gggcgtgggc ttcgttccgt actcgccgtt ggggcgtggc
      121 ttcctgaccg gcaccgtccg ctcggcagcc gatctcgcgt cgaacaacga cttccgcaag
      181 acgatgcccc gcttcgccga gggtgccctc gatgcgaacc tcgtggtcgt cgacgtcgtc
      241 acgtcggtgg ccgatgcgtt gggcgcgacg gccgcccagg tggcgctggc ctggttgcgg
      301 caccgcgccg aaaccttcgg tgtggcgtcg gtgccgattc cgggaacccg cagggccgca
      361 cgggtcgagg agaatctcgg ctcgttgtgg gtgacgctga gcggggatca gctcaccgca
      421 ttggatgccg ccgcagacgc cgtgtccggg aacaggtccc gcgatctgaa ctgggtgtcg
      481 gccggccgcg agtagccggt cacggcggca tggtctgcca cgcgcgaagg tggtggacgg
      541 cgaacccgtc gaacaacggc tgctgaagcc gtgcggcgac ggcggtgagc tgcgtgcgca
      601 tcaccgcctc gccgtcgtcg gcgaacgtcg tgttggtcgg taccgaggat gacggcggct
      661 gggtctcgac gccgatccgg tagcgagttc ccgcggtttc acagagccgg agcatcggtt
      721 cggcctccgc gacgatggtc gaggcacggt ctcggtaggc cagcacggtg atcgaatcac
      781 actgacgcac aatgcgagtg agcaactcac ggttcgacgt cgaggtggac ggcgtcgaac
      841 acggcgtcgg tggtggcccg gaacgcccaa ttggacgcgg tgtcatgctg gaccgtccac
      901 gtcgggtcgc cgccgaggca cgacaccgca attccgttgg cccgcaacgc tgtggtgagc
      961 gccgcgacgt tgtcgtcgac cccgctgagt ggcaccgcga cgtagacctc gcgaagccgg
     1021 ggcgcggcca cgaactcgac gatcttcggg atgtctgctc ctgcttgcca cagccacgtt
     1081 gcgaccgttt ccgccggatc cgcgtccaca gatctcacgt taggcaattt ctggcgatat
     1141 ggcctgagcc ggaccgttcg acggggtagg tatgtctgat gatcgaagtc accctgctcg
     1201 gcaccggcag tccgattccc gacccgaacc gggcgggccc gtcgacgctg gtgcgggccg
     1261 gtgggcagac gttcctggtc gactgcggtc gtggcgtgca acagcggttg gcggcggtgg
     1321 gttcaggtgc caatgcgttg tccgcactgc ttctcaccca tctgcacagc gatcacatag
     1381 ccgacctcgg ggacgtgatc atcacgcgtt gggtgacgac attcgatccg gacgcgccgg
     1441 ccttgccgat catcggaccg cccggcacgg cagaagtcgt cgacgccacg ctcaaagcgt
     1501 tcggccccga catcggctac cggattgcac accaccccga catcaccggc cctccgcgcg
     1561 tcgaggtgga ggaggtcacc ggcggcgtgg tgtgggggcg cgtcggcgtg cggatccggg
     1621 tggcgctgac cgatcaccgg ccggtggcgc cgaccatcgg gttccggatc gaacatgacg
     1681 acgcgtcggt ggtgctggcc ggcgacaccg tgccgtgcgc cggcctcgac gacctggccg
     1741 cgggcgcagg cgcgctcgtg cacacggtga tccgcaaaga gctgatctcg cagatgccgc
     1801 agcagcggat ccgcgacatc tgcgattacc actcgtcggt cgaggaggcc gctgccaccg
     1861 cggagcgcgc gggcgtcggc atcctcgtgc tcacccacta cgtgccgggc atcgcacccg
     1921 ggcaagagga cgactggcgc gccttggcgg cgacggtgtt cagcaggcag atcgaactcg
     1981 gcgacgatct gcaccgcgtc gaggtgcacc ccggcgtatg cggtggccgc ggcggcaact
     2041 aaccgataag ggcgcgtagc gcctcggcgg ccggctcgta cgtcatgacg gtgaagtggt
     2101 cggcgtcgac gtcggtaatg gtgagcgcct cgaccgtcga gtcgatatcg gcggcgacgt
     2161 cgtcgggaac cacaaggccg ccgttgagtg gctgcctgca gcgcaccaat ccgacgggca
     2221 tggtcaggtg gcgccactgg tgcggccatt ctcgcccgga cgcgtcgcgg ccatcttcga
     2281 ggcaggcagc cttggtagtc cgccggaatt cgtaggcata gaagcgccgc cagaagtcgg
     2341 tgaaggggcg gatcggactg acggcctcga cctgcgacac gtacccttcg gcagtcgaaa
     2401 ggtcgagatc cagccggttg aggccggcga gtacggccgc cacggctcct tcgtcggacc
     2461 ggcccgcatg gtcgatcagc ccgaggcggc ggatacggcc cggttcgagc tccgcggcga
     2521 ggatgccggt cagcgcgccg agcgaccagc cgacccagtc gaactgctga tgtcccagaa
     2581 gcgttgccgc ctcgaacatg tcacgggcat gggacgccag accataggtg cccggtgccg
     2641 tggagtcgct gccgccgcgt ccgcgcaagt cgaccgcgac gacctggcgt ccgccgtcgg
     2701 ccaccagatg cggagccagg tagtcgaagc cgtgcatgtt cgccgaaagt ccgtgcaccg
     2761 cgagcaccag cggcgcatcg ggatcgccga agcgttgagc gtgcacgcgc ccggacgtga
     2821 g
//
LOCUS       DASYLX010000370         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_16946, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000370 DASYLX010000000
VERSION     DASYLX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16946"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGP27_09120"
     CDS             <1..664
                     /locus_tag="VGP27_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851508.1"
                     /translation="SHQQQSMILVPIDTPGVSIERSLPVFGWQDQHGHCEISFDNVRV
                     PVSNLLDEEGSGFAIAQARLGPGRIHHCMRAIGVAERAMALMIDRVQTRIAFGKPLAE
                     QGVVQQQIAQSRNEIDQSRLLCHKAAWTIDQHGNQSREARQLVSMIKAVAPQMACNVI
                     DRAIQVHGAAGISDDTVLARLYGWHRAMRLFDGPDEVHMRTIARGELGKEKSAFAAAV
                     TS"
     gene            664..1452
                     /locus_tag="VGP27_09125"
     CDS             664..1452
                     /locus_tag="VGP27_09125"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEV7851509.1"
                     /translation="MPPLSGTWNFRDVAEKGGIQPGRLFRSGELSNLDDSGRETLGRL
                     GITDVADLRSAPELERHGKGLVPDNVVIHHLPFVETIASDADAPHEHAFQKMLTEKSD
                     DESVSDAAARYMTEEYDRIARSTLAQRAVHKVVTMLGDDDRVLAHCFAGKDRTGFTIA
                     VVLEAAGVHRDAVMADYLASNDAVPELRAHIVETIRDRMSETPEVAEVAEARLTDAVL
                     GVQEGYLESARRAILDDFGSLDGYLGAAGVTAEDVTRLRAALLG"
     gene            complement(1449..>1712)
                     /locus_tag="VGP27_09130"
     CDS             complement(1449..>1712)
                     /locus_tag="VGP27_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851510.1"
                     /translation="AEESRQTHRDAVRRVREAQTFAVTLPVLGRVRLPRPEQLAFYGA
                     LGALAAVEIIEWPVALVLGVGHALMQNEHSRVAHEVGEALEDA"
BASE COUNT          303 a          554 c          600 g          255 t
ORIGIN      
        1 aagccatcag cagcagtcga tgatcttggt gcccatcgac acacccggtg tgtcgatcga
       61 gcggtcgctg ccggtgttcg gctggcagga ccagcacggg cactgcgaaa tcagcttcga
      121 caacgtccgg gtgcccgtgt ccaatctgct cgacgaggag ggcagcggct tcgcgattgc
      181 acaggcgcgg ctcggccccg gacgcatcca tcactgcatg cgggcgatcg gggtggccga
      241 gcgggcgatg gcgctgatga tcgaccgggt gcagacgcgc atcgctttcg gcaagccgct
      301 ggcggagcag ggcgtggtgc agcagcagat tgcccagtcc cgcaacgaaa tcgaccagag
      361 ccgtctgttg tgccacaagg cagcgtggac catcgaccag cacggcaacc agagcagaga
      421 agcgcgccaa ttggtttcga tgatcaaggc ggttgcaccg cagatggcgt gcaacgtcat
      481 cgaccgggcg atccaggtgc acggcgcagc cggcatcagc gacgacaccg ttctggcgcg
      541 gctttacggc tggcatcggg ccatgcgact gttcgacggc cccgacgagg tgcacatgcg
      601 gaccatcgcg cgaggcgagc tcggcaagga gaaatcagcc ttcgcggcgg cggtgacgag
      661 ctagtgccgc cgctttcggg gacatggaac tttcgcgacg tcgccgagaa gggcgggatc
      721 caaccgggca ggttgttccg gtccggcgag ctgagcaacc tcgacgactc gggccgcgaa
      781 acccttggcc ggctgggcat caccgatgtg gcggacctgc ggtcggcgcc ggagttggag
      841 cgccacggca aggggctggt gcccgacaac gtggtgatcc accacctgcc gttcgtggaa
      901 acgatcgcct ccgacgccga cgcgccacac gagcacgcct tccagaagat gctgacagag
      961 aagtccgatg acgagtcggt cagcgacgcc gccgcccgct acatgaccga ggaatacgac
     1021 cggatcgccc ggtcaaccct cgcgcagcgc gcggtgcaca aggtggtcac gatgctcggt
     1081 gacgatgaca gggtgctggc ccactgcttc gccggaaagg accggacggg gttcaccatt
     1141 gcggtggtgc tcgaggcagc gggcgtgcac cgcgacgccg tcatggccga ttatctggcc
     1201 agcaatgacg ccgtgccgga gctgcgcgcc cacatcgtcg agacgatccg cgaccggatg
     1261 tcggagacac ccgaggtcgc ggaggtggcc gaggcccggc tcaccgatgc ggtgctcggt
     1321 gttcaggagg ggtatctcga atcggcgagg agggcgatcc tcgacgactt cggttcgctg
     1381 gacggctacc tcggcgccgc aggcgtgaca gccgaggatg tcacgaggct gcgcgccgca
     1441 cttcttggtt aggcgtcctc gagcgcctcg ccgacctcgt gcgcgacgcg gctgtgctcg
     1501 ttctgcatca acgcatggcc cacgccgagc accagggcaa ccggccactc gatgatctcc
     1561 accgccgcca gcgcccccag cgcgccgtag aaggcgagtt gctcgggacg cgggaggcgc
     1621 acgcggccga gcacgggaag cgttaccgcg aacgtctgcg cctcgcggac ccgtcgcacc
     1681 gcgtcgcgat gggtctgtcg ggattcttca gc
//
LOCUS       DASYLX010000371         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1662766,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000371 DASYLX010000000
VERSION     DASYLX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662766"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGP27_09135"
     CDS             <1..328
                     /locus_tag="VGP27_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEV7851511.1"
                     /translation="AATDGSLRVDVTDENVYVTSGLADDLADGPDSVDREQLELAVEL
                     LRDIGDYAEDDIVDETLDADMPLGRFVAHVLDPNTVRKPSGPYAEAVEQWESLETFLE
                     SRLRQE"
     gene            complement(331..678)
                     /locus_tag="VGP27_09140"
     CDS             complement(331..678)
                     /locus_tag="VGP27_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEV7851512.1"
                     /translation="MITGSHVIVYSRDADADRAFFADVLDYPHVDAGGGWLIFKLPPG
                     EVAVHPTDGPQSHELYLMCDDINATVEQLAAKGVTCSEVNDQGWGLLTSIRLPSGGEL
                     GLYEPRYARATEL"
     gene            complement(678..1223)
                     /locus_tag="VGP27_09145"
     CDS             complement(678..1223)
                     /locus_tag="VGP27_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851513.1"
                     /translation="MKIPMNALCGATAASLLAIGVTIGGMGSAVAQPDYGAIPVHPND
                     VIDATAFTAAAPVINPDGQAGVSTVYTHRDGTRQITSTVLVLADPAAASASMDGSRAE
                     LAGQVVDSKSQPAAVGSGGTIVSGLSADRSQSVSVLTFTEGSAFANVEFQGPTNDPVP
                     GDLVTEYGQRQDTAIRNALAA"
     gene            complement(1238..1474)
                     /locus_tag="VGP27_09150"
     CDS             complement(1238..1474)
                     /locus_tag="VGP27_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851514.1"
                     /translation="MRKIVMTVAGGAMLLGSLTTAQIAQAAQTSATHDTIVYTQPAAP
                     EVHALDCRGGTGAMGCGPGWFWRDGWRGWACYPC"
BASE COUNT          263 a          530 c          497 g          252 t
ORIGIN      
        1 cgccgccacg gacggctcac tgcgcgtcga cgtcaccgac gagaacgtct acgtgacgag
       61 cggcctggcc gacgacctcg ccgacggacc cgactccgtc gaccgggagc agctggagct
      121 cgccgtcgaa ttgctcaggg atatcggcga ttacgccgag gacgacatcg tcgacgagac
      181 gctggacgcc gacatgccgc tcggccggtt cgtcgcccac gtgctggatc cgaacaccgt
      241 ccgcaagccg agcggtccgt acgcggaggc cgtcgagcag tgggagtcgc tggaaacctt
      301 cctcgagtcc cggctgcgcc aggagtagtc ctacagctcc gtggcgcgtg cgtaccgcgg
      361 ctcgtagagg ccgagttcgc cgccgctggg cagccggatg ctggtcaaca gtccccaccc
      421 ctgatcgttg acctcggaac aggtcacccc tttggcagcg agctgctcga cggtggcgtt
      481 gatgtcgtcg cacatcaggt acagctcatg cgactgcgga ccgtcggtgg gatgcaccgc
      541 cacctcgcca ggcgggagct tgaagatcag ccagccgccg ccggcgtcga cgtgcggata
      601 gtcgagcaca tcggcaaaga atgcccggtc ggcgtcggcg tcgcggctgt agacgatcac
      661 atggctgccc gtgatcacta ggcggccagc gcgttcctta tcgcggtgtc ctgccgctgg
      721 ccatactcgg tgaccaggtc gccgggaacc ggatcgttgg tcgggccctg gaactcgacg
      781 ttggcgaatg cgctgccctc ggtgaacgtc agcacagaga ccgattgcga tcggtccgcg
      841 gaaagcccgc tgacgattgt tcctccggat cccaccgcgg ccggctgcga cttcgagtcg
      901 acgacctggc cggccagctc cgcacgggaa ccgtccatcg aagccgacgc cgccgcggga
      961 tctgccagca caaggaccgt gctggtgatc tgacgggtgc catcgcggtg ggtgtacacc
     1021 gtcgagacac ccgcctgtcc gtccgggttg atcacgggcg cggcggcggt gaacgccgtc
     1081 gcatcgatca cgtcgttcgg gtgaaccggt atcgcaccat agtcgggttg cgctacggca
     1141 gagcccatcc cgccgatcgt cacaccgatc gccaacagcg atgccgccgt cgcgccacat
     1201 agggcgttca tgggaatctt catcgtctcg tctccaatca gcaggggtag caggcccagc
     1261 cgcgccagcc gtcgcgccag aaccaccccg gaccgcaacc catcgcaccc gtcccgcccc
     1321 tgcagtcgag cgcgtggact tcgggcgcag ccggctgcgt gtagacgatg gtgtcgtgtg
     1381 tagcactcgt ctgcgcggcc tgcgcaattt gcgcggtcgt caaggagccc aacaacattg
     1441 cccccccggc gaccgtcatg acgatttttc gcatctgctt cctccctcga cgatctgatg
     1501 aaagtgcgag cagacgcaaa gttacgcgac acgccgacgc ga
//
LOCUS       DASYLX010000372         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1553443,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000372 DASYLX010000000
VERSION     DASYLX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553443"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VGP27_09155"
     CDS             complement(<1..1029)
                     /locus_tag="VGP27_09155"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV7851515.1"
                     /translation="MSVLLFGVSHRSAPVSVLEQLSTDESDQAKIVEQALQSSLVTEA
                     MVLSTCNRVEVYAVVDAFHGGLSVIGQVLAEHSGMGLGDLTKYAYVRYAEAAVEHLFA
                     VASGLDSAVIGEQQVLGQVRRAYAAAETNHTVGRTLHELAQRALSVGKRVHTETGIDA
                     AGASVVSVALDMADGKLSGLAGRRAVIIGAGAMGALSAKHLVRAGVERVHIVNRTLPR
                     AKRLAEKIRAMGVTADAFPFDHLTPVLTDADVVVSCTGAVRPVVSLADVHRGLAHGQE
                     PKQMVICDLGMPRNVDPAVAGLPGVFVVDMDRIQRDPAARAASSDTEAARAIVAAEVA
                     NYLAGQRMA"
     gene            complement(1098..1355)
                     /locus_tag="VGP27_09160"
     CDS             complement(1098..1355)
                     /locus_tag="VGP27_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV7851516.1"
                     /translation="MNRAQVVLLTRAGCSLCEKTAARLAELAGELDFHVTITDVDAAA
                     AAGDTALRAEYGDRLPVVLLDGREHSYWELDEPRLRADLAK"
     gene            1404..>2138
                     /locus_tag="VGP27_09165"
     CDS             1404..>2138
                     /locus_tag="VGP27_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEV7851517.1"
                     /translation="MSDGDEAAVQALAGDASAETAVAHLAEDDDAAPAAPPPDLTAAA
                     FFDVDNTLVQGSSLVHFARGLAARKFFTYGDLARFGYAQAKFQLTGRENSDDVAAGRR
                     KALAFIEGRSTAELVAVGEEIFDEIIADKIWPGTRALAQMHLDAGQQVWLVTATPYEL
                     AATIASRLGLTGALGTVAESVDGVFTGRLVGDILHGSGKAHAVRSLAIREGLNLRRCT
                     AYSDSFNDVPMLSLVGTAVAINPDADL"
BASE COUNT          327 a          761 c          726 g          324 t
ORIGIN      
        1 ggccatccgc tgtccggcca ggtagttcgc cacttcggcg gcgacgatcg cgcgcgccgc
       61 ctcggtgtcc gatgaggccg cgcgggcggc cgggtcgcgc tggatccggt ccatgtcgac
      121 gacgaacacg ccgggcaggc ctgcgaccgc gggatcgacg ttgcgcggca tgccgaggtc
      181 gcagatcacc atctgcttgg gctcctgacc gtgggccagg ccgcggtgca cgtcggccag
      241 cgagaccacc ggacgcactg cgccggtgca gctgacgacg acgtcggcat ccgtgagcac
      301 cggcgtcagg tggtcgaacg ggaacgcgtc ggcggtgacg cccatcgcgc gaatcttctc
      361 ggcgagccgc ttggcccgtg gcagggtcct gttcacgatg tgcactcgct cgacgcccgc
      421 gcgcaccagg tgcttcgcgg acagggcgcc catcgcgcct gccccgatga tcaccgcacg
      481 gcgcccggcc agcccgctca gcttgccgtc ggccatgtcc agcgcgacgg acaccaccga
      541 cgcgcccgcc gcgtcgatac cggtctcggt gtgcactcgc ttgccgaccg acagcgcgcg
      601 ctgagccagc tcgtgcaggg tgcgcccgac agtgtgattg gtctcggcgg cggcgtaggc
      661 gcggcgcacc tgcccgagca cctgctgttc gccgatcacc gccgaatcca ggccgctggc
      721 aaccgcgaac aggtgctcga cggccgcctc ggcatagcgg acatacgcgt atttggtgag
      781 gtcgccaagg cccatgccgg aatgttcggc gagcacctgc ccgatcaccg agaggccgcc
      841 gtggaaggcg tcgacgacgg cgtagacctc cacgcggttg caggtggaca gcaccatcgc
      901 ctcggtcaca agtgaggact gcagcgcctg ctcgacgatc ttggcttgat cggattcgtc
      961 ggtgctcaac tgctcgagga cggacaccgg cgcgctgcgg tgcgaaaccc cgaaaagcag
     1021 cacgctcatg gcctcgtcac cacgtcatcc acggtagccg ttcaccagct gggctaccaa
     1081 attgcagcca acggcggtca cttcgcgaga tcggccctca acctgggctc gtcgagctcc
     1141 cagtagctgt gctccctgcc gtcgagcagc accaccggca gccggtcgcc gtactcggcg
     1201 cgcagcgcgg tatcacccgc cgcggccgcg gcgtcgacgt cggtgatcgt cacgtggaag
     1261 tccagttcgc ccgccagctc ggccagccgt gcggcggtct tctcgcacag cgaacagccc
     1321 gctctggtca gcaacaccac ctgcgcccgg ttcacgcaac cagtatcggc tccaatccgg
     1381 tgtcttagtg ttgacaggtg cccattagcg atggtgacga agccgccgtt caggcgttgg
     1441 cgggcgatgc cagcgccgag acggccgtcg cccatctcgc cgaagacgac gacgccgccc
     1501 cggcggcgcc gccgccggat ctgaccgcgg ccgccttctt cgacgtcgac aacacgctgg
     1561 tgcagggctc gtcgctggtg catttcgccc gcggcctggc ggcccgcaag ttcttcacct
     1621 acggcgacct cgcgcgcttc ggctacgcgc aggcgaagtt tcagctcacc ggccgggaga
     1681 acagcgatga cgtggcggcg ggtcggcgca aggcgctggc gttcatcgag ggccggtcta
     1741 cagcagaact cgtcgcggtc ggcgaagaga tcttcgacga gatcatcgcg gacaagatct
     1801 ggccgggcac ccgcgcgctg gcgcagatgc acctggacgc gggtcagcag gtgtggctgg
     1861 taaccgcgac gccgtacgag ttggccgcga cgatcgccag ccggctgggg ttgaccggcg
     1921 cgctgggcac cgtcgccgaa tcggtggacg gggtgttcac cggccgactg gtcggcgaca
     1981 tcctgcacgg atccggtaag gcccacgccg tgcggtcgct ggccatccgc gagggcctga
     2041 acctgcgccg ctgcaccgcg tactcggaca gcttcaacga cgtgccgatg ctgtcgctgg
     2101 tcggcacggc cgtggccatc aaccccgacg ccgacctg
//
LOCUS       DASYLX010000373         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1332679,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000373 DASYLX010000000
VERSION     DASYLX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332679"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGP27_09170"
     CDS             complement(<1..270)
                     /locus_tag="VGP27_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005573538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV7851518.1"
                     /translation="MAETESTFTVFVAVAVNLAVAIAKTVAAAVTGSASMTAEAAHSW
                     ADTGNQGLLIVANRRSSRPPDAERPLGYGREAYVWSLLAAVGLFVV"
     gene            288..1367
                     /locus_tag="VGP27_09175"
     CDS             288..1367
                     /locus_tag="VGP27_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV7851519.1"
                     /translation="MTNTGPLAGVKVIELGGIGPGPHAAMMLADLGADVVRVRRPGGL
                     TMPAEDVDLLHRGKRIVDLDVKQQPQALLKLAAKADVLLDCFRPGTCERLGIGPEDCA
                     AVNPRLIFARITGWGQQGPLAMTAGHDINYLSQTGALSAIGYRDRPPVAPLNLVADFG
                     GGSMFALVGIVAALYERERSGEGQVVDAAMVDGVSILSQMMWTMKATGSLRDERESFL
                     LDGGAPFYRTYETSDGRYMAVGAIEPQFFAQLLTGLGLTPDEVPSQFEIGAYPQMHKI
                     FTERFASRTRDEWTDVFAGTDACVTPVLTWTEAAQNEHLRARSTVMENGGVEQAAPAP
                     RFSRTPSGPVGTPPKGTTALDEIGW"
     gene            1417..1860
                     /locus_tag="VGP27_09180"
     CDS             1417..1860
                     /locus_tag="VGP27_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851520.1"
                     /translation="MAVRASREVVFDVPPEVILDVLADIDSVPSWSALHKTAEVLDRH
                     PDGRPHHVKATLKIMGITDKEILEYHWGERWMVWDSSETSAQRGLHGEYNLTPEGEDR
                     TRVRFDIILELAAPVPAFLVKRAKKMVLDVATEALRERVMASGST"
     gene            1896..2813
                     /locus_tag="VGP27_09185"
     CDS             1896..2813
                     /locus_tag="VGP27_09185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851521.1"
                     /translation="MPIGIVLVERSNQSNVVDDFVDQARRAHEIGVRQVWLAQRFDHD
                     ALALAGLVGAAVPGLGVGTFVVPINPRHPLLVASLAQTAQAASHGNFSLGLGLGAHEP
                     ERQAFGTAWPNTIARLREHLTVLRSVFDEGAVDFHGDEFTANPAWPVAVPGGTPIPIY
                     VAAMGPKALRVTGELADGTLPYLAGPRTISEFIEPTITKAAADTGRPGPRIIAAVPVL
                     VTDDVDAGRSFAADQLGFYQTIPSYQRVIAREGVQSAAELAAVGPAASVVRKLKSYLD
                     AGATDVVLSPLDRSEGADHESLWTVAASL"
     gene            2851..>3094
                     /locus_tag="VGP27_09190"
     CDS             2851..>3094
                     /locus_tag="VGP27_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7851522.1"
                     /translation="MPSRRRSALVDKLVVDQPGRPQQAILDELRRVILDGAVPPGTPI
                     PLADVADLFGVSQIPVRESLKTLIGEGLVSHRSNAGY"
BASE COUNT          501 a         1032 c         1052 g          509 t
ORIGIN      
        1 gacgacgaac aagccgaccg ctgccagcag tgaccagacg taggcctccc gcccgtatcc
       61 cagtggacgc tcggcgtcgg gtggtctgct gcttcgccga ttggccacga tcagcagccc
      121 ctgattgccg gtatcggccc aggaatgcgc ggcctcggcc gtcatggatg ccgagccggt
      181 caccgcggcc gcgaccgttt tggcgatcgc caccgcgagg ttgacggcga cggccacgaa
      241 gacagtgaag gtactttcgg tttctgccac cccgctagtc tgcgaccgtg accaacacgg
      301 ggcccctcgc gggggtgaag gtgatcgagc tcggcggcat cggacccggg ccgcatgcgg
      361 caatgatgct cgcggatctc ggcgccgatg tggtgcgggt gcgtcggccc ggcggtctga
      421 cgatgcccgc cgaggacgtc gacctgctgc accgcggcaa gcgcatcgtc gacttggacg
      481 tcaagcagca gccgcaagcg ctcctgaagc tggccgccaa ggccgacgtg ctgctcgact
      541 gcttccggcc gggcacctgc gagcggctcg gcatcgggcc cgaggactgc gccgcggtca
      601 atccgcggct gatcttcgcg cgcatcaccg gctggggtca gcagggtccg ctggcgatga
      661 ccgcgggcca tgacatcaac tacctgtcgc agaccggcgc actgtccgcg atcggctacc
      721 gggaccggcc gccggtcgct ccgctgaacc tggtcgccga cttcggcggc ggctccatgt
      781 tcgcgcttgt cggcatcgtc gccgcgctct acgaacggga gcggtccggc gaaggccagg
      841 tggtcgacgc cgccatggtc gacggtgtga gcatcctgtc gcagatgatg tggacgatga
      901 aggcgacggg ttctctgcgc gatgagcgtg agtcgttcct gctcgacggc ggtgcaccgt
      961 tctaccggac atacgagacg tccgacggca ggtacatggc cgtcggcgcg atcgagccgc
     1021 agttcttcgc gcaattgctg accggcctcg gattgacgcc cgatgaggtg ccaagccagt
     1081 tcgaaatcgg cgcctatccg cagatgcaca agatcttcac agagcggttc gcgagcagga
     1141 cccgcgacga gtggaccgac gtcttcgccg gcaccgacgc ctgcgtgacg ccggtgctga
     1201 cgtggaccga agccgcgcaa aacgagcacc tcagagcccg ttccacggtg atggagaacg
     1261 gcggtgtcga acaggctgcg cctgcgcccc ggttctcccg cacgccgtcc gggcctgtcg
     1321 gcaccccgcc gaaggggacc accgcgctcg atgaaatcgg ttggtagcac cctcgttggc
     1381 cgccgcccgc gccgctcgag gattacattc gccgacatgg ccgttcgagc gtcgcgggag
     1441 gtcgttttcg atgtcccacc cgaggtgatc ctcgatgtgc tcgctgacat cgactccgtg
     1501 ccgtcgtggt cggcgctgca caagacggcc gaagtgctcg accgccatcc ggacggcagg
     1561 ccgcatcacg tcaaggccac gctgaagatc atggggatca ccgacaagga gatcctcgaa
     1621 taccactggg gcgagcgatg gatggtgtgg gactcctcgg agacctccgc gcagcgggga
     1681 ctgcacggtg agtacaacct gacgcccgaa ggcgaggaca ggacccgggt gcgcttcgac
     1741 atcatcctcg aactggccgc acccgttccc gctttcctgg tgaagcgcgc caagaagatg
     1801 gtgctcgacg tggcgacgga agccctgcgc gagcgggtca tggcgagcgg atcgacctag
     1861 ggaattgtcg cgtgcacggg ccggttgctc gaagtatgcc gatcggaata gtgctcgtcg
     1921 aacgctcgaa ccagtccaat gtggtcgatg atttcgtcga ccaggctcgg cgggcacacg
     1981 agatcggtgt ccgacaggtt tggctggctc agcgcttcga ccacgacgcc ctggcgctcg
     2041 ccgggctcgt gggggcggcg gtgcccggcc ttggtgtcgg gaccttcgtg gtgccgatca
     2101 acccacggca tccgcttctc gtggcgtcac tggcgcagac ggcgcaggcc gcctctcacg
     2161 gcaatttcag tctcggtctc gggctgggtg cccacgagcc ggaacgccag gcgttcggca
     2221 ccgcgtggcc caacaccatc gcgcgccttc gtgagcacct gaccgtgttg cggtcggtgt
     2281 tcgacgaggg ggctgtcgac ttccacggcg acgagttcac ggcgaacccc gcgtggcctg
     2341 tcgcggttcc cggtggtacg ccgatcccca tctacgtcgc cgcgatgggc cccaaggcat
     2401 tgcgcgtgac cggcgaattg gccgacggca cactgcctta cctcgcgggg ccgcggacga
     2461 tatcggagtt catcgaaccg acgatcacga aggcggccgc cgatacgggc agaccgggtc
     2521 cgcgcatcat tgccgcggtt ccggtgctgg tgaccgacga cgtcgacgcc gggcgaagct
     2581 tcgcggccga ccagttgggc ttctaccaga ccatcccgtc ctaccaacga gtcatcgccc
     2641 gagaaggtgt gcagagcgcc gccgaactcg cggccgtcgg gccggccgcg tcagttgtcc
     2701 gcaagctgaa gagctacctc gacgcaggcg cgaccgatgt cgtgctcagc ccgctggacc
     2761 gttcggaggg cgccgatcac gaatcgctgt ggacggtcgc cgcctcgctg tagcaacact
     2821 ggcgaaacaa acgctgctta ccgtgtcgtg atgccgtctc gacgacgctc ggcgttggtg
     2881 gacaagctcg tcgtcgatca gcccggccgg ccgcaacagg ccatcctcga cgagctgcgc
     2941 cgggtgattc tcgacggggc ggtgcctccg gggaccccga tcccactggc cgacgtcgca
     3001 gatctgttcg gcgtcagcca gataccggtc cgcgagtcgc tcaagacgtt gatcggtgag
     3061 gggctggtgt cgcaccgcag caacgcaggc tatg
//
LOCUS       DASYLX010000374         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3197418,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000374 DASYLX010000000
VERSION     DASYLX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197418"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VGP27_09195"
     CDS             complement(<1..1361)
                     /locus_tag="VGP27_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7851523.1"
                     /translation="MTSTWSGRRTKSVEQSIEDTDEPGTRLRKDLTWFDLIVFGVSVV
                     IGAGIFTITASTAGGITGPAISISFIFAAVACGLAALCYAEFASTVPVAGSAYTFSYA
                     SFGEVVAWIIGWDLVLEFAVGAATVAKSWSTYLGEVFGFAGGELHLGGLNLDWGAALI
                     VGIVTTLLAIGTKLSSRVSAVITAIKVAVVLVVIIVGAFFIKAANYTPFVPPAEQGGS
                     SGDQSLFSLLTGAGSSNYGWYGVLAGASIVFFAFIGFDVVATTAEETKNPQRDVPRGI
                     FGTLAIVTVLYVAVAVVLSGMVKYTELEKAGEDANLATAFSLNGVDWAATLISVGALA
                     GLTTVVMVLMLGQIRVLFAMSRDRLLPHQLAQTGERGTPVKLTILVGVLVAAAAAFFP
                     ITKLEEMVNVGTLFAFVLVSAGVIILRRNRPDLHRAFRTPLVPWLPIAAIVACVWLML
                     NLTA"
     gene            1435..2814
                     /locus_tag="VGP27_09200"
     CDS             1435..2814
                     /locus_tag="VGP27_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851524.1"
                     /translation="MGRNAKHAVVVGASIGGLCAARVLSDFYDRVTVYERDDLPAQPA
                     NRPAVPQGRHLHLLLARGALELDAHFPGLLDDMVAAGVPILENRPDCIHFGAAGHVLG
                     TTRELQDEFTAYVPSRPHLEWQIRRRVTAIENVDIVRAGVGEPTFDAARQRVTGVRLD
                     TGEQIPADLVVDSAGRGTRLPVWLKQWGFDRPPEHSVDVGISYATHQLRIPEGVIAEK
                     VVVAGAFSGQPRGLGMLCYEDRTWVLTTFGVAKAEPPHDFPGMCALADELVPPHIADA
                     IRKGEPIGDVAFHKYPTSRWRRYHKLDRFPAGILPFGDAVASFNPTFGQGMTMISLQA
                     GHLRRALESPGSDLAAEFNAATAKSTFPVWTMNAIGDLVMHGATGPMPWWYRPVGGLF
                     DQFLGASETDPLLAEWFLRRFSLLDSLYMVPSMKLVGRTIRHNMALFVAEWRASSRSA
                     SDRDPVPGR"
     gene            complement(2771..3691)
                     /locus_tag="VGP27_09205"
     CDS             complement(2771..3691)
                     /locus_tag="VGP27_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7851525.1"
                     /translation="MSAEGSTRAILAALLANAGIAVAKFVGFLITGSSSMLAEAVHSV
                     ADTSNQGLLLLGQRQSRKEADLLHQFGYGRSRYFYSFVVALVLFTLGSVFALYEGYHK
                     ISHPAELTSPIVAIVILVVAIGLEGYSFRTAVKESRPLKRSGSWWRFIRTSRNPELPV
                     VLLEDSGALVGLVLALIGVGLTVLTGNAVWDGIGTVGIGLLLGVIAVVLMVEMHSLLI
                     GEAATGEEDQAIRAALEQTDNIDRVIHLRTQYLSPEELLVGAKIALAAATDLATVATA
                     IDTAEAAIRAAVPAAQVIYLEPDLDRTPTG"
     gene            complement(3692..>4396)
                     /locus_tag="VGP27_09210"
     CDS             complement(3692..>4396)
                     /locus_tag="VGP27_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV7851526.1"
                     /translation="GQPDADGLRALFTTWITAPQPDLDMLVPAVIDGAIDYVRSGDKR
                     FAAEARTVLELGERYPGDAGVLAALLLNRFTLRPGEGIYLPAGNLHTYLHGVGIEVMA
                     NSDNVLRGGLTPKHVDVPELLRVLDFAPATEASLRPKTVEDGMETVYQTAAPEFSVSV
                     LGIDGERLGHEVDAPSRHDGPQILLCTEGSAVVHAKSSAVTLERGAAAWVSADDGPIR
                     LVAQRPAKLFRATVGI"
BASE COUNT          778 a         1543 c         1402 g          673 t
ORIGIN      
        1 gccgtcaggt tcagcatcag ccacacgcag gcgacgatcg cggcgatcgg cagccagggc
       61 accagcggcg tccggaaggc gcggtggagg tcgggacggt tgcgccgaag gatgatgacg
      121 cccgccgaga ccaacacgaa cgcgaacaac gtgccgacgt tgaccatctc ctcgagcttg
      181 gtgatgggga agaacgccgc cgcggccgcc accagaacgc cgacgaggat cgtcagcttg
      241 accggcgtgc cgcgctcgcc cgtctgtgcg agttgatgcg gcagcagccg gtcccgtgac
      301 atcgcgaaca gcacccggat ctggcccagc atcaacacca tgacgacagt cgtcagcccc
      361 gccagtgcac cgacgctgat cagggtggcc gcccagtcga ccccgttgag gctgaacgcc
      421 gtcgcaaggt tggcatcctc gcccgccttc tcgagctcgg tgtatttgac cattcccgaa
      481 agcaccaccg ccaccgcgac atagagcacg gtgacgatgg caagtgtgcc gaagattccg
      541 cggggcacgt cgcgctgcgg gttcttggtc tcctcggccg ttgtcgcgac gacgtcgaat
      601 ccgatgaacg cgaagaagac gatcgatgcg cccgccagta cgccgtacca gccgtagttg
      661 ctgctgcccg ccccggtcag caacgagaaa agcgactggt cacccgacga accgccctgc
      721 tccgccggcg gcacgaacgg cgtgtagttc gccgccttga tgaagaacgc tccgacgatg
      781 atgacgacaa gcaccaccgc caccttgatc gcggtgatga ccgcgctgac gcgtgacgac
      841 agcttggtgc cgatcgcgag caatgtggtc acgatgccga cgatcaacgc cgccccccag
      901 tcgaggttca gcccgccgag gtgcagctcc ccgcccgcga aaccgaacac ctcacccaga
      961 tacgtcgacc agctcttggc caccgtcgcc gcgccgacgg cgaactcgag caccaggtcc
     1021 cagccgatga tccacgccac cacctctccg aagctggcat aggagaaggt gtaggcactg
     1081 cccgccacgg ggacggtgga ggcgaactcg gcgtagcaca gcgccgccag cccacaggcg
     1141 acggccgcga agatgaacga gatggagatg gcgggtccgg tgataccgcc cgcggtggac
     1201 gcggtgatgg tgaagattcc ggcgccgatg acgaccgaga cgccgaagac gatcagatcg
     1261 aaccaggtga ggtccttgcg caatcgggtc ccgggttcat cggtgtcctc gatcgactgc
     1321 tcgacagact tggttcgccg acccgaccat gtgctcgtca tccgccttac cctttcgtgg
     1381 cgccgtccgc gcatcggccg gctgggctcg gagtacccac tacgatcggc gcccatgggg
     1441 cggaacgcga agcacgcggt cgtcgtcggg gccagcatcg gggggctgtg cgccgcgagg
     1501 gtgctctctg acttctacga cagagtcacc gtctatgaac gcgacgacct gcccgcccaa
     1561 ccggccaacc ggccggccgt gccccagggc agacacctgc acctgctatt ggcccgcggc
     1621 gcactggaac tcgacgcaca cttccccggc cttctcgacg acatggtcgc cgcgggggtg
     1681 ccgatcctgg agaaccgacc ggactgcatc cacttcggcg ccgcgggtca cgtgctgggc
     1741 acgacgcgcg agctgcagga cgagttcacg gcgtacgtgc cgagccggcc acacctggaa
     1801 tggcagatcc ggcgcagggt gacggccatc gaaaacgtcg acatcgtgcg cgcaggtgtc
     1861 ggtgagccca ccttcgatgc ggcccgccaa cgcgtgacgg gcgtgcggct cgacaccggt
     1921 gagcagatcc cggccgatct cgtcgtcgac tccgcgggcc gcggcacccg gttaccggtc
     1981 tggctgaagc agtggggatt cgaccgccct cccgagcaca gcgtcgacgt cggcatcagc
     2041 tatgccaccc atcagctgcg catacccgag ggcgtgatcg ccgagaaggt cgtcgtcgca
     2101 ggcgctttca gcgggcagcc gcgcggcctt ggcatgctct gttacgagga ccgcacctgg
     2161 gtcctgacca ccttcggtgt ggccaaggca gagccacccc acgatttccc cggaatgtgc
     2221 gctctggccg acgaactcgt gcccccgcac atcgccgacg ccatccgcaa gggcgagccc
     2281 atcggcgatg tggcgttcca caagtacccg acgagccggt ggcgccggta ccacaaactg
     2341 gaccgcttcc cggccgggat cctgccgttc ggcgacgccg tcgccagctt caatcccacc
     2401 ttcgggcagg gcatgacgat gatctcgctg caggcgggtc atctgcgccg ggcactcgag
     2461 tcaccgggca gcgacctggc cgccgagttc aacgccgcca ccgcgaagtc gaccttcccg
     2521 gtgtggacga tgaacgctat cggagacctg gtcatgcacg gcgccacagg gccgatgccg
     2581 tggtggtacc gccccgtcgg tggcctcttc gatcagttcc tcggcgcgtc ggagaccgat
     2641 ccccttctcg cggaatggtt cctgcgccgg ttcagcttgc tggacagcct gtatatggtc
     2701 ccgtcgatga agctggtcgg ccggaccatc cggcacaaca tggcgctgtt cgtggccgag
     2761 tggcgggcct ctagccggtc ggcgtccgat cgagatccgg ttccaggtag atgacctgtg
     2821 cggcgggcac cgccgcgcgg atcgccgctt ccgccgtgtc gatggcggtg gccacggtcg
     2881 ccaggtccgt cgccgctgcg agcgcgatct tggccccgac gagaagctct tcggggctca
     2941 ggtactgggt gcgcagatga atcacccggt cgatgttgtc ggtctgctcg agcgccgccc
     3001 ggatcgcctg atcctcttcc ccggtggcgg cctcgccgat cagcaggctg tgcatctcca
     3061 ccatcaggac gacggcgatg acgccgagca gcagcccgat gccgacggtg ccgatcccat
     3121 cccacaccgc attccctgtg agcacagtga gcccgacgcc gatcagcgcg aggaccaggc
     3181 cgacgagtgc gccggagtcc tccagcagca ccaccggcag ctcgggattg cgcgacgtgc
     3241 ggatgaaccg ccaccagctg ccggagcgct tcagcggccg cgattccttc accgccgtgc
     3301 gaaagctata gccctcgagg ccaattgcga cgaccaggat cacgatcgcc acgatcggcg
     3361 acgtcagctc cgccgggtgc gaaatcttgt gatagccctc gtagagcgcg aacaccgagc
     3421 ccagcgtgaa caggaccagc gccaccacga acgagtagaa gtaacggctg cggccgtagc
     3481 cgaactggtg cagcaaatcg gcttccttgc gggactggcg ctgccccaac aacagcagac
     3541 cctggttgct ggtgtcggcg accgagtgca ccgcctcggc cagcatcgac gagctgccgg
     3601 tgatcaggaa gccgacgaac ttcgccacgg caatgcccgc attggcgagc aaggccgcca
     3661 gaatcgccct tgtgctgcct tcggccgaca tctagatgcc cacggttgcg cggaacagct
     3721 tggccggccg ctgagcgacc agccgaatcg gtccgtcgtc ggccgaaacc cacgccgccg
     3781 cgccccgctc caatgtcacc gcgctggatt tggcgtgcac cacggccgaa ccctccgtgc
     3841 acaacaaaat ctgcgggccg tcatggcgcg acggcgcatc cacctcgtga ccgaggcgct
     3901 ccccgtcgat gcccaacacc gacaccgaga actccggcgc cgcagtctga tacaccgttt
     3961 ccattccatc ctcaaccgtc ttcggccgca ggctcgcctc ggtggcgggc gcgaagtcga
     4021 gcacccgcaa cagctccggc acgtcgacgt gtttcggagt cagcccgccg cgaaggacgt
     4081 tgtccgagtt cgccattact tcgatcccga caccgtgcag gtaggtatgc agatttccgg
     4141 cgggcagata gatgccttcg ccggggcgca gcgtgaaccg gttcaacagc agggcggcca
     4201 gtacaccggc gtcgccaggg tagcgctccc ccagttccag caccgtcctg gcctccgccg
     4261 caaatcgttt gtcccccgaa cggacgtagt cgatcgcgcc gtcgatcacc gcgggcacca
     4321 acatatcgag atccggttgc ggcgcggtga tccatgtggt gaacagcgcc cgcaggccgt
     4381 ccgcgtcggg ttggcc
//
LOCUS       DASYLX010000375         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2430384,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000375 DASYLX010000000
VERSION     DASYLX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430384"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VGP27_09215"
     CDS             <1..390
                     /locus_tag="VGP27_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEV7851527.1"
                     /translation="PLLASLRPELMYLMQPLGGEYAGTRELLTAVPFAPGYGVEIGLL
                     IDTYDRLGLDAIAQVNLGVREHRNRPLTELASMSRQVIATLLSRCGIPDSGVGLTQFF
                     ADGYDYTPRTSEVSLADRPPMLTLRPR"
     gene            442..810
                     /locus_tag="VGP27_09220"
     CDS             442..810
                     /locus_tag="VGP27_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEV7851528.1"
                     /translation="MTLILLYLVVLILVAIVLFGVGSVLFGRGEVLPPLPRATTATAL
                     PASGVTGADVDAVKFSQAVRGYKTSEVDWVLDRLGQELDLLRGQLAAVQATQVGDEHP
                     DEEPIDGGVHALPADERADS"
     gene            807..1400
                     /locus_tag="VGP27_09225"
     CDS             807..1400
                     /locus_tag="VGP27_09225"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV7851529.1"
                     /translation="MTKAPADDGRVRCGWIDQSRLAPDDFILYRDYHDTEWGQPLRDS
                     KSLFERVSLEAFQSGLSWLIILRKRENFRRAFHRFDIDRVARYTDRDIERLMNDAGIV
                     RNRSKIEATIANARAVADLDVDLGELLWSFAPDPPRPRPADFASVPAVTPESTAMAKE
                     LKRRGFKFVGPTTAYALMQATGMVDDHVASCWVPAAA"
     gene            1540..1707
                     /locus_tag="VGP27_09230"
     CDS             1540..1707
                     /locus_tag="VGP27_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEV7851530.1"
                     /translation="MAAMKPRTGDGPLEATKEGRGIVMRVPLEGGGRLVVELTPDEAA
                     ALGDELKGVTS"
     gene            complement(1704..2870)
                     /gene="glgA"
                     /locus_tag="VGP27_09235"
     CDS             complement(1704..2870)
                     /gene="glgA"
                     /locus_tag="VGP27_09235"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEV7851531.1"
                     /translation="MRVAMMTREYPPEVYGGAGVHVTELVAQLRNLCEVDVHCMGAPR
                     EGAFVHQPDPALKGANPALATLSADLVMANAARDATVVHSHTWYTGLAGHLAALLYGV
                     PHVLTAHSLEPMRPWKAEQLGGGYRISSWVERTAVEAADAVIAVSSGMRDDVLHTYPA
                     LDPNRVHVVRNGIDTEVWYPVQPQDSQESVLAELGVDPTRPIVAFVGRITRQKGVAHL
                     VAAAHRFAPEVQLVLCAGAPDTPEIAAEVTAAVQELSRARTGVFWVREMLPISKIREI
                     LSAATVFVCPSVYEPLGIVNLEAMACATAVVASDVGGIPEVVADHQTGLLVHYDPGDP
                     GFFETRLADAVNSLVADPQRARQYGEAGRQRCIEEFSWAHIAEQTLEIYRKVSS"
     gene            3019..4233
                     /gene="glgC"
                     /locus_tag="VGP27_09240"
     CDS             3019..4233
                     /gene="glgC"
                     /locus_tag="VGP27_09240"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV7851532.1"
                     /translation="MRELPHVLGIVLAGGEGKRLYPLTADRAKPAVPFGGAYRLIDFV
                     LSNLVNARYLRICVLTQYKSHSLDRHISQNWRLSGLAGEYITPVPAQQRLGPRWYTGS
                     ADAIYQSLNLIYDEDPDYIVVFGADHVYRMDPEQMVQFHIESGAGATVAGIRVPRAEA
                     TAFGCIDSDESGRIRAFVEKPADPPGTPDDPEQTFVSMGNYIFTTKVLIDAIRADADD
                     DDSDHDMGGDIIPRLVGDGMAAVYDFKNNEVAGATERDHGYWRDVGTLDAFYDAHMDL
                     VSVHPVFNLYNKRWPIRGESENLAPAKFVNGGSAQESVVGAGSIISAASVRNSVMSSN
                     VVVDDGAIVEGSVLMPGVRVGRGAVVRHAILDKNVVVGPGEMVGVDLEKDRERFAISA
                     GGVVAVGKGVWI"
     gene            complement(4276..5133)
                     /locus_tag="VGP27_09245"
     CDS             complement(4276..5133)
                     /locus_tag="VGP27_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7851533.1"
                     /translation="MTGAEPVDFEAHRDHLHGVAFRMLGSRSEADDAVQETWLRMNRA
                     VPADVANPRGWLTTVVARICLDMLRSRSARPEEPLDEADHPGETVNPEDHAVLADSVG
                     VALMLVLQTLAPDERLALVLHDVFDVPFAEIAPIIDRSANATAQLTVRARRRVRGADL
                     EQGAGVADERRVVEAFLAAARDGDFNGLLALLHPDIELRADAAAAGGNPVLVRGGVEV
                     ASRASMFAANSAFAEVALVDGAPGVVVAPEGELTLVLRFASTAGVIVSIDICADPIRL
                     ARFDFAVFG"
     gene            5157..5591
                     /locus_tag="VGP27_09250"
     CDS             5157..5591
                     /locus_tag="VGP27_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851534.1"
                     /translation="MSDDPQSRFMTRSTDMYRPLRIVLLVHGLITLAGAFVLTVFPTA
                     IPSAVGITVERPDYLLVYLVGAAELAVAVLSFGATRLTDPAALGLVVMTFVVLHGASG
                     ILDVVYMAVTDVNGTMIANTVARFTVVVVFILAWRTTKRHQT"
     gene            complement(5579..>5704)
                     /locus_tag="VGP27_09255"
     CDS             complement(5579..>5704)
                     /locus_tag="VGP27_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV7851535.1"
                     /translation="VAGLAVLALRINDEEALLTQQLAGYREYMQKVHYRLVPYVW"
BASE COUNT          912 a         1917 c         1939 g          936 t
ORIGIN      
        1 cccctgctgg cctcgctgcg gcccgagctg atgtacctga tgcagccgtt gggcggcgag
       61 tacgcgggca cccgcgagct gctgacggcg gtgccgttcg cacccggcta cggcgtcgag
      121 atcggcctgc tcatcgacac ctacgatcgg ctcggcctgg acgcgatcgc gcaggtgaat
      181 ctgggtgtgc gcgagcaccg caaccggccg ctgaccgagc tcgcctcgat gagcaggcag
      241 gtcatcgcca ccctgctgtc ccgctgcggc atcccggact cgggggtcgg cctgacgcag
      301 ttcttcgccg atggctacga ctacacgccc cgcacgtcgg aagtgtcgct ggccgaccgg
      361 ccgccgatgc tcactctgcg gccgcgctag cgcgggggtg gtccactctc ttcgtcggac
      421 cggtgaggca gtatcggaag cgtgaccctg atcctgctgt atctggtggt gctgatcctg
      481 gtggcgatcg tgctgttcgg ggtaggcagc gtgctgttcg ggcgtggcga ggtgttgccg
      541 ccgctgcccc gcgccaccac cgcgacggcg ttacccgcgt ccggcgtcac cggcgccgac
      601 gtcgacgcgg tcaagttcag ccaggcggtg cgcggctaca agaccagcga ggtcgactgg
      661 gtgctggaca ggctgggcca ggaactcgac ctgctgcgcg gtcagctcgc cgcggtgcag
      721 gccacccagg tgggtgacga gcatcccgac gaggagccga tcgacggtgg cgtgcacgcg
      781 ctgccggccg acgaacgggc cgattcatga cgaaggcgcc ggcggacgac ggccgcgtcc
      841 gatgcggctg gatcgaccaa tcccgtctgg cgccggacga ttttattctc taccgcgact
      901 accacgacac tgagtggggg cagccgctgc gtgattccaa gtcgttgttc gagcgggtca
      961 gcctcgaggc gttccagagc gggctgtcgt ggttgatcat cctgcgcaaa cgggagaact
     1021 tccggcgtgc ctttcaccgg ttcgacatcg acagggtcgc ccgatacacc gaccgcgaca
     1081 tcgagcggct gatgaacgac gcggggatcg tgcgcaaccg ctcgaagatc gaggcgacca
     1141 tcgccaacgc gcgcgccgtc gcagacctgg acgtcgacct cggcgagttg ctttggtcgt
     1201 tcgcaccgga cccgccgcgg ccacgacccg cggactttgc cagcgtgccc gcggtgaccc
     1261 ccgagtcgac ggcgatggcc aaggagctga agcgtcgcgg cttcaagttt gtgggtccga
     1321 cgactgctta tgccctgatg caggccaccg ggatggtcga cgaccacgtt gcttcctgct
     1381 gggtccccgc cgccgcctga tccgcacgtt gaaacgatct tgaccgggtc tttgcacatc
     1441 gaacgccccc gatagggaac aatggaggaa gttcagtagc agttcaacgc cgggagctgt
     1501 cgacgggtgg gcagccggct catgtggagg gagcactcga tggcggcgat gaagccccgg
     1561 accggcgacg gtcctctgga agcaaccaaa gaggggcgcg gcatcgtgat gcgggtacca
     1621 ctggaaggcg gtggccgcct cgtcgtcgaa ctcacccctg atgaggccgc cgccctcggc
     1681 gacgagctca agggcgtcac cagctaactg gacaccttcc ggtagatctc cagagtctgc
     1741 tcggcgatgt gggcccacga gaattcttcg atgcagcgct gcctgccggc ctccccgtac
     1801 tggcgcgcgc gctgcgggtc ggcgaccaac gagttgaccg cgtcggccag ccgggtctcg
     1861 aagaagcccg gatcacccgg gtcgtagtgg accaatagcc cggtctgatg gtcggccacc
     1921 acctcgggga tgccgccgac gtccgaggcg accaccgccg tcgcgcacgc catcgcctcc
     1981 aagttcacga tgcccaacgg ctcgtacacc gacgggcata cgaataccgt tgctgccgaa
     2041 aggatttcgc ggatcttgct gatcggcaac atctcgcgca cccagaacac gccggtcctg
     2101 gcgcgcgaaa gctcctgcac cgcggctgtc acctcggcgg cgatctccgg agtgtccggc
     2161 gcgcccgcgc agagcaccaa ctgcacctcg ggagcgaatc tgtgcgcggc cgccaccagg
     2221 tgcgcgacgc ccttctgccg ggtgatccgg ccgacgaacg ccacgatcgg gcgggtgggg
     2281 tccaccccga gctcggccag caccgattct tgtgaatcct gcggctgaac cgggtaccag
     2341 acctcggtgt cgatgccgtt gcgcaccacg tgcacccggt tggggtccag cgcggggtag
     2401 gtgtgcagga cgtcgtcacg catcccggag ctgaccgcga tgacggcgtc ggccgcctcg
     2461 accgcagtgc gttccaccca ggacgagatg cggtaaccac cgccgagctg ttcggccttc
     2521 cacggccgca tcggttcgag cgagtgggcg gtcagcacat gcggaacgcc gtacagcagc
     2581 gccgccaggt gcccggccag cccggtgtac caggtgtgcg agtgcaccac ggtcgcgtcc
     2641 cgggcggcgt tggccatcac cagatcggct gacagcgtcg ccagcgccgg gttggcgccc
     2701 ttgagcgccg ggtcgggctg gtggacgaaa gcgccctcgc ggggggcgcc catgcagtgc
     2761 acgtcgacct cgcacaggtt gcgtaattgt gcgacgagtt cggtgacgtg gacgccggcc
     2821 ccgccgtaca cctccggcgg atactcacgc gtcatcatcg ccacccgcat gcttacgacc
     2881 gtagtggccg tggccggacc tcgcagctat gtaagaacgc cgatttgggt aatcaccaat
     2941 tactttgacc gacagccgca caggtctcaa tcgtgcgaat gcctggtcag catcaacacc
     3001 ggcggatagg tttgggtcat gagggagttg ccacacgtcc tcggcatcgt cctggcgggc
     3061 ggggaaggca agcggctgta tccgctgacg gcggaccgag caaagccggc cgttcctttc
     3121 ggcggtgcct accggctgat cgatttcgtg ctgtccaatc tggtcaatgc ccgatacctg
     3181 cggatctgcg ttctcacgca atacaaatcg cattccctgg accggcacat ctcgcagaac
     3241 tggcgactgt cgggcctggc cggcgaatac atcaccccgg tgcctgcgca gcagcggctg
     3301 gggccgcgct ggtacaccgg ctcggccgac gcgatctacc agtcgctgaa cctgatctac
     3361 gacgaggacc ccgactacat cgtcgtgttc ggcgcggacc acgtgtaccg gatggatccc
     3421 gagcagatgg tgcagttcca catcgagagc ggcgcgggtg cgacggtcgc cggaatccgg
     3481 gtgccgcggg ccgaggccac cgcgttcggc tgcatcgact ccgacgagtc gggccgcatc
     3541 cgcgctttcg tcgagaagcc cgccgatccg ccgggcactc ccgacgaccc cgagcagacg
     3601 ttcgtgtcga tgggcaacta catcttcacc accaaggtgc tgatcgacgc gatccgcgcc
     3661 gacgccgacg atgacgactc cgatcacgac atgggcggcg acatcattcc gcggttggtg
     3721 ggcgacggca tggcggcggt ctacgacttc aaaaacaacg aggtggcggg ggccaccgag
     3781 cgcgaccacg gctactggcg cgacgtcggg acgctggacg cgttctacga cgcccacatg
     3841 gatctggtgt ccgtgcaccc ggtgttcaac ctgtacaaca agcgctggcc gatccggggc
     3901 gagtcggaga atctggcgcc cgccaagttc gtcaacggcg gctccgctca ggagtcggtg
     3961 gtcggcgcgg gcagcatcat ctcggcggcg tcggtgcgca actcggtgat gtcgtccaac
     4021 gtggtcgtcg acgacggcgc gatcgtggag ggcagtgtgc tgatgccagg tgtccgggtg
     4081 ggccgcggcg cggtggtgcg ccacgcgatc ctcgacaaga acgtcgtcgt cgggcccggt
     4141 gagatggtcg gtgtcgactt ggagaaggac cgggaacggt tcgcgatcag cgcaggcggc
     4201 gtggtggccg tcggcaaggg cgtctggatc tgatcgcggt tggtggcttg gcggaaccgt
     4261 gctgctgact gcggctcagc cgaacaccgc gaagtcgaac cgcgccagcc gtatcgggtc
     4321 tgcgcagatg tcgatactga cgatgacgcc cgcggtgctg gcgaagcgca gcaccagggt
     4381 cagctcgccc tcgggcgcga cgacgacacc gggtgcgccg tcgacgagcg ccacctccgc
     4441 aaaggccgag ttggccgcga acatcgaggc ccgcgacgcc acctcgacgc cgccccgcac
     4501 cagcaccgga ttcccgcccg cggccgcggc gtcggcacgc aactcaatgt cggggtgcaa
     4561 caacgccagc aggccattga agtcgccgtc gcgcgcggcg gccaggaacg cctcgacgac
     4621 gcggcgttcg tccgcgacgc ctgcgccttg ttctaagtct gcgccgcgta cgcgccgcct
     4681 ggcccgcacc gtcagctgag ccgtcgcgtt ggcggaccgg tcgatgatcg gagcgatctc
     4741 ggcgaacggc acgtcgaaga cgtcgtgcag caccagcgcc aggcgttcat cgggggccag
     4801 cgtctgcagc accagcatca gtgcgacgcc gacggaatcc gccagcacgg cgtggtcctc
     4861 gggattgacc gtctcacccg gatgatcggc ctcgtcgagc ggctcctcgg ggcgggcgga
     4921 tcgcgaccgc aacatgtcca ggcagatccg cgcgacgacc gtcgtgagcc agccgcgggg
     4981 gttggccacg tcggccggga cggcgcggtt catccgcagc caggtctcct gcaccgcgtc
     5041 gtccgcctcg ctgcgtgacc ccagcatgcg gaaggccacg ccgtgcaggt ggtcgcggtg
     5101 cgcctcgaag tccaccggtt ccgctcccgt catgttcgac gacgtttcat cgtcatatga
     5161 gtgacgatcc gcagagccgt ttcatgacaa ggagcaccga catgtaccgc ccacttcgca
     5221 tcgtgctgtt ggtgcacggc ctcatcacgc tcgccggcgc cttcgtgctg acggtgttcc
     5281 cgaccgcgat tccgtcggcg gtaggcatca cggtcgagcg gccggattat ctgctcgtct
     5341 acctcgtcgg ggccgccgag ttggcggtcg cggtgttgtc gttcggcgcg acccgcctga
     5401 cggatccggc cgcgctgggg ctcgtcgtga tgacgttcgt cgtccttcac ggcgccagcg
     5461 ggatcctcga tgtcgtctac atggcggtta ccgacgtgaa cggcacgatg atcgccaaca
     5521 ccgtcgcgcg gttcaccgtc gtggtggtgt tcattctcgc ctggcggaca acgaagcgtc
     5581 accagacgta gggcaccagc cggtagtgca ccttctgcat gtactcgcgg taaccggcca
     5641 actgctgggt cagcagcgcc tcctcgtcgt tgatccgtag ggccagcacg gccaggccgg
     5701 cgac
//
LOCUS       DASYLX010000376         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_785013,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000376 DASYLX010000000
VERSION     DASYLX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785013"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2019)
                     /locus_tag="VGP27_09260"
     CDS             complement(4..2019)
                     /locus_tag="VGP27_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851536.1"
                     /translation="MRYVVTGGTGFIGRRVVSRILDRFPDAQVWVLVRRESLTTFERL
                     AAQGDLPWGDRAKALIGDLTAEGLGLSEDSIVELGSVEHVVHCAAVYDITVAEDLQRA
                     ANVEGTRAVITLARELGATLHHVSSIAVAGSYRGEYTEDDFDVAQDLPTPYHQTKFEA
                     EILVRSEPGLRYRVYRPAVVVGDSRTGEMDKVDGPYYFFPILAKLAVLPSFTPVLLPD
                     TGRSNIVPVDYVVDAMTELIHAPDRDGQTFHLTAPDSIGLRGIYRGVSRAAGLPRLRG
                     SLPRGAVTPFLRATGRAKVLRNMAVTQLGIPAEILDVVDLMPTFTAENTRNALQHTGI
                     AVPEFSSYAPKLWRYWAEHLDPDRARRDDPAGPLVGKHVIITGASSGIGRAAAIAVAA
                     RGATVFALARNAEALDDLVAEIRAAGGEAHAFTCDVTDGTSVEHTVKDILGRFDHVDY
                     LVNNAGRSIRRSVVASTDRLHDYERVMAVNYFGAVRMVLALLPHWRERRFGHVVNVSS
                     AGVQARNPKYSSYLPTKAALDAFSDVVSTETLSDHITFTNIHMPLVQTPMIAPSRRLN
                     PVPPITAEHAAAMVVRGLIEKPARIDTPLGTLADVGNYFTPKLSRRVLHQLYLGYPDS
                     AAARGIEKGPSPRTPSRRPRRPARTVARVRMPRPVKRAVRLVPGVHW"
     gene            complement(2074..2652)
                     /locus_tag="VGP27_09265"
     CDS             complement(2074..2652)
                     /locus_tag="VGP27_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851537.1"
                     /translation="MAGVPDAQRANGSALTEKQQHDPVQWMRLYWEQQNREDPGAFLV
                     MTSVLRLHHLMTNALEHNLRTKFKISLTDYQILTALQLSETGTWLLSRMAWHLMVHAT
                     TVTLAVERLETKRHVTRHSHPHDKRTTLVTITDEGRQLADAATDALARMDFGLPGLTA
                     PQARSLIATIGRLRAGAGDVDRSYESASTDLA"
     gene            2738..3607
                     /locus_tag="VGP27_09270"
     CDS             2738..3607
                     /locus_tag="VGP27_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV7851538.1"
                     /translation="MPIDPTAIGAKTEPQVFEWTDRDTLLYALGVGAGTDDLAFTTEN
                     SHDIPQQVLPTYAVIACTGFAAAGKIGSFNFAMLLHGSQEIRLFEPLPPAGKLSVVGE
                     VADIQDKGEGKNAVVMLKATGTNPDTGKVVAETMATAVIRGEGGFGGQPGQRPVAPEI
                     PDRDPDSRIALPTREDQALIYRLSGDRNPLHSDPWFARELAGFPKPILHGLCTYGVAG
                     RALVSELGGGDATKVTSIASRFTSPVFPGETLTTSIWRTEAGRAVFRTEAANPDGSDA
                     RLVLDDGVVEYTD"
     gene            3637..4518
                     /locus_tag="VGP27_09275"
     CDS             3637..4518
                     /locus_tag="VGP27_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7851539.1"
                     /translation="MKLVVGFLATPGGADAVALGVRLARTLGAQLELCLVLPPDRAET
                     ARLSVGTFDSLLAEQAQKWLKEAVIPADVVARSHVSFAESFADGLLREIARLEADAIV
                     VGGAGGGLAGPLSLGSVVNELLHSSPVPVAVAPRGTRHSDVERVREVTCAIGQRQGAD
                     LLLRTAVRFSKAAGTPLRLASLVALDPMFGRLRGDADAVRDRALAHAQHTLDIAKDAL
                     PETIPVTSTVVDGATVEDAVSKLDWRDGDLIMVGSSRLSAPRRLFLGSTAAKMLRVLD
                     VPMMVVPRDQIDGDALP"
     gene            4698..>5398
                     /locus_tag="VGP27_09280"
     CDS             4698..>5398
                     /locus_tag="VGP27_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7851540.1"
                     /translation="MGWHNGPPSWTEMERVLHSKPRRAGWPIDEQIGDGGDSPAWSRK
                     RGSYQPPEPHWRRHDARDVQRPGSSVPYAELHAHSAYSFLDGASTPEELVEEAARLDL
                     RAIALTDHDGLYGVVRFAEAAKELDVKTVFGAELSLAGGDRTEDPDPRGPHLLVLARG
                     PEGYRRLSRQLASAHLAGGEKGVLRYDYDALTEAAGGHWHILTGCRKGHVRQALSDGG
                     PEAAERALADLVDRFG"
BASE COUNT          870 a         1774 c         1860 g          894 t
ORIGIN      
        1 cggctaccag tgcacgccag gcaccaaccg aaccgcgcgc ttgacgggac gcggcatgcg
       61 tactcttgcc accgtccttg ccggccgtcg tggccgccgc gacggtgtgc gcggagatgg
      121 gcccttttcg atgccccgag ccgccgccga gtcgggatag cccaggtaaa gctggtgcag
      181 cacccgccgc gagagtttcg gggtgaagta gttgccgacg tcggccaggg tgccgagcgg
      241 ggtgtcgatt cgcgccggct tctcgatcag gccacgcacc accatggcgg cggcgtgttc
      301 ggcggtgatc ggcggcaccg ggttgaggcg gcgcgacggc gcgatcatcg gtgtctgcac
      361 caacggcatg tggatgttgg tgaacgtgat gtggtcggac agcgtctccg tcgacaccac
      421 gtccgagaag gcgtccagcg ccgccttggt cggcaggtac gagctgtact tcgggttgcg
      481 ggcctgcaca ccggcgctcg acacgttgac gacgtgaccg aaccggcgtt cgcgccagtg
      541 cggcagcagc gccaatacca tccgcacggc gccgaagtag ttgaccgcca tcacccgctc
      601 gtagtcatgc agccgatcgg tcgacgcgac caccgatcgc cggatcgatc tccccgcgtt
      661 gttgacgagg tagtcgacgt ggtcgaagcg tccgaggatg tccttgaccg tgtgttccac
      721 cgacgtgccg tcggtgacgt cgcacgtgaa cgcatgcgcc tcgccgcctg cggcgcggat
      781 ctcggcgaca aggtcgtcga gcgcttctgc gttgcgggcc agcgcgaaga ccgtcgcacc
      841 gcgtgcggcc accgcgatcg ccgcggcccg gccgatgccg ctggacgccc cggtgatgat
      901 gacgtgcttg ccgaccagcg gacctgcggg atcgtcgcgc cgcgcccgat cgggatccag
      961 gtgctcggcc cagtaacgcc acagcttggg cgcgtacgac gagaactccg gcacagctat
     1021 tccggtgtgc tgcagagcgt ttcgcgtgtt ctcagcagtg aacgtcggca tcaggtcgac
     1081 gacgtcgagg atctcggcgg ggatgcccaa ctgagtgacc gccatattgc gcagcacctt
     1141 ggcccgaccc gtcgctcgga gaaacggcgt cacggcaccg cgggggagcg acccgcgcag
     1201 ccgcggcaac cccgcagccc gtgaaacgcc gcggtagata ccgcgcagtc cgatgctgtc
     1261 gggtgcggtg aggtggaatg tctggccgtc gcgatcgggc gcgtgaatca attcggtcat
     1321 ggcgtcgacg acgtagtcga ccggcacgat gttggaccgt ccggtgtcgg gcagaagaac
     1381 cggggtgaac gagggcagca cagccagctt cgcaaggatg ggaaagaagt agtacgggcc
     1441 gtccaccttg tccatctcgc ctgtgcgcga gtcgcccacc accaccgcgg gccggtagac
     1501 gcggtagcgc aggccgggct ccgagcgcac caggatctcg gcttcgaact tggtctggtg
     1561 gtacggcgtc gggaggtctt gcgcgacgtc gaagtcgtct tcggtgtatt cgccgcgata
     1621 gctgcccgcc accgcgatcg acgagacgtg gtgcagcgtc gcaccgagct cgcgcgcgag
     1681 cgtgatcacc gcccttgtgc cctcgacgtt cgcggcgcgc tggagatcct cggcgacggt
     1741 gatgtcgtag accgcggcgc agtgcacgac gtgctcgacg gaaccgagtt cgacgatgga
     1801 gtcctcggag aggcccaggc cttcggcggt caggtcgccg atcagcgctt tggctcgatc
     1861 accccacggc aggtcgccct gggcggccag ccgctcgaac gtcgtcaacg actcccggcg
     1921 gaccaggacc cacacctgcg cgtcggggaa gcgatccaga atccgtgaca cgactcggcg
     1981 cccaataaac ccggtaccgc cggtaacgac atagcgcatg cggccatgct cgtccgtcgt
     2041 ccgacaaagg tcaacgcagt gtgatgcggt cgatcaggcc aggtcagtgg acgccgactc
     2101 gtacgaccgg tcgacatcgc ccgccccggc gcgaagccga ccgatggtcg cgatcagcga
     2161 ccgcgcctgc ggggcggtga ggcccggtag tccgaaatcc atccgagcca gcgcgtcggt
     2221 cgccgcgtcg gcgagttggc ggccctcgtc ggtgatcgtc accagggttg tccgcttgtc
     2281 gtggggatgt gagtgtcgtg tgacatgccg tttcgtttcg aggcgctcga cggcgagggt
     2341 gaccgtggtg gcgtgcacca tcaagtgcca cgccatcctg ctcaacagcc aggtcccggt
     2401 ttcgctcagt tgcagcgccg tgagaatctg gtagtcggtc aggctgatct tgaacttcgt
     2461 ccgcaggttg tgttcgagcg cgttcgtcat cagatggtgc aaccgcagca ccgaggtcat
     2521 gaccagaaaa gcgcccggat cttcgcggtt ctgctgttcc caatagagcc gcatccactg
     2581 caccggatcg tgctgctgct tctcggtcag cgcagatccg ttcgcccgtt gggcgtctgg
     2641 taccccggcc accatgtatc ggtagccaaa aaagtggccg ctcaatcccc tcgagcgcgg
     2701 gacggatatg gtccgaggca gtccgaaggg agtgtcaatg ccgatcgacc cgaccgcgat
     2761 aggcgcgaaa accgaaccgc aggtcttcga atggaccgac cgagacacgc tgctctacgc
     2821 gctcggcgtc ggcgccggca ccgatgacct cgcattcacc accgagaaca gccacgacat
     2881 cccgcaacag gtgctgccga catatgccgt catcgcctgc accggcttcg cggcggccgg
     2941 caagatcggc agcttcaact tcgcgatgct gctgcacggg tcgcaggaga tccgactgtt
     3001 cgagccgctg ccaccggccg gaaagctgag cgttgtcggc gaagtggccg acatccagga
     3061 caagggcgaa ggcaagaacg ccgtggtcat gctcaaggcg accggcacca acccggacac
     3121 cgggaaggtc gtggccgaga cgatggcgac cgcagtcatc cgcggcgaag gcggcttcgg
     3181 cggtcagccg gggcagcggc cggtggcgcc cgagatccct gaccgcgatc ccgattcgcg
     3241 gatcgccctg ccgacccgcg aggatcaggc cttgatctac cggctgtccg gcgaccgcaa
     3301 tccgctgcac agcgatccat ggttcgcgcg cgaattggcc ggcttcccca agccgatcct
     3361 gcatgggctg tgcacctacg gggtggccgg ccgtgcgctg gtcagcgagc tcggcggcgg
     3421 cgacgcgacg aaggtcacct cgatcgcctc gcgcttcacc tcacccgtct ttcccggcga
     3481 gacgctcact acctcgatct ggcgcaccga ggcgggccgc gcggtgttcc gtaccgaggc
     3541 ggcgaacccg gacggctcgg acgcccggct ggtactcgac gacggcgtcg tcgaatacac
     3601 cgactgagca gccgtcggtg gtacaggatg tccaccatga agctggtcgt cggtttcctg
     3661 gccacacccg gtggcgcgga cgcggttgct ctcggtgtcc ggctggcccg cacgctgggc
     3721 gcgcagttgg agctttgcct cgtgctgccg cccgatcgcg cggaaaccgc gcgcctttcg
     3781 gtcggcacct tcgacagtct tctcgccgag caggcgcaga agtggctgaa agaggccgtg
     3841 ataccggccg acgtcgtcgc gcgtagccac gtcagtttcg ccgagtcgtt cgccgacggt
     3901 ctgcttcggg agatcgcgcg gttggaggcg gatgccatcg tcgtcggcgg cgccggtggt
     3961 gggcttgccg gaccgctctc gctgggctcg gtcgtcaacg agctgctgca ctcgtcgccg
     4021 gttccggtgg ccgtggcgcc gcggggcacc cgccattccg acgtcgagcg ggtccgcgag
     4081 gtgacgtgcg cgatcggcca gcgtcagggt gcggatctgt tgttgcgcac cgcagttcga
     4141 ttcagcaagg cggcgggcac accgttgcgg ctggcgtcac tggtggctct cgacccgatg
     4201 ttcggccgcc ttcgcggcga tgccgacgcc gtccgggacc gggcgctcgc tcatgcgcag
     4261 cacaccctgg atatcgccaa agacgctctg ccagagacga ttccagtgac atcgacggtc
     4321 gtcgacggtg ccaccgttga ggatgcggtc agcaagctcg attggcgcga cggtgacctg
     4381 atcatggtcg ggtcgagccg gctcagcgcg ccgaggcggt tgttcctcgg ctcgacggcg
     4441 gccaagatgc tgcgcgtcct cgacgtgccc atgatggtcg tgccccgcga tcagatcgac
     4501 ggcgacgcgc tgccgtaggc gttacccggg cgcgccgccg atgcggcgac gagtcgcatc
     4561 gatcaacgtt ttgcagaggt cttctcgcac tcgttggaaa acgtcgatcg gcgtgcctta
     4621 tgtgacatgt cgggcacagc gtcttaggac aggatcttag gactggcgtt cgaacaagcg
     4681 ttcgatacaa tgtcgcaatg ggttggcaca acgggccgcc gagctggaca gagatggagc
     4741 gggtgcttca cagtaagccc cgtcgtgccg gctggcccat cgacgagcag atcggcgacg
     4801 gcggcgacag cccggcctgg tcccgcaaga gggggagcta tcagccgccg gagccgcact
     4861 ggcggcgaca tgatgctcgc gacgtgcagc ggcccggctc ctctgttccc tacgccgaac
     4921 tgcatgcgca ttcggcgtac agctttctcg acggcgccag cacgccggag gaactcgtcg
     4981 aggaggcggc ccggctggat ctgcgcgcca tcgcgctgac cgaccacgac gggctctacg
     5041 gcgtggtgcg gttcgccgaa gccgccaagg aactcgacgt gaaaacggtg ttcggtgccg
     5101 aactgtccct cgcaggcggc gaccgtaccg aggatcccga tccccgtggg ccgcacctgc
     5161 tggtgcttgc ccgcggtccc gaggggtatc gacggctgtc acgccagttg gcgtcggcgc
     5221 atctcgccgg cggcgaaaag ggggtattgc gctacgacta cgacgcgctc accgaggcgg
     5281 cgggcggaca ctggcacatt ttgaccggct gccgcaaagg ccatgtccgc caagcgctat
     5341 ccgatggcgg ccccgaagcg gcggaaaggg cgcttgccga tctggtggac cggttcgg
//
LOCUS       DASYLX010000377         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1332722,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000377 DASYLX010000000
VERSION     DASYLX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332722"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VGP27_09285"
     CDS             complement(<1..1368)
                     /locus_tag="VGP27_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEV7851541.1"
                     /translation="MDWLIAPQWWWGRLVLERGVAAVYVVAFVCAARQFRGLIGERGM
                     LPVPDYLAHRSFRNTPSLFHLHYSDAFFASVCWVGATISAAIAAGLADAVPVWAACLL
                     WLLLWVLYLSIVNVGQIWYSFGWESLLLEAGLLVVFVGNDDVGPPALVMWLTRWLLFR
                     VEFGAGLIKLRGDRCWRQLTCLDYHHETQPMPGPLSWFFHHLPRPFHRMEAAGNHLAQ
                     LIVPFGLFAPAPIASAAATIIAVTQFWLVLSGNFAWLNWLTIVLAVSVIDTSSLTTML
                     GITSPTDIPATQPYWYAALVIAFTALVVVRSWYPARNLISRRQRMNSTYDPLHMVNTY
                     GAFGSINRTREEVIIEGTSSSDVSGETVWAEYEFRGKPGDVKRLPRQWSPYHLRLDWL
                     MWFLPISPAYAQTWLIPLCRGLLTNEPSIVRLLRTNPFPDTPPVQIRATLYRYRFSTA
                     HELRSE"
     gene            complement(1391..1729)
                     /locus_tag="VGP27_09290"
     CDS             complement(1391..1729)
                     /locus_tag="VGP27_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851542.1"
                     /translation="MTTPESQARVSRKQTVVNWGLALSTIIGALLIEAYSFMRVMGTA
                     GCSDRACANTGPGNFLYGLITYGAPVVALAAIVISSFTARRPRGWAVPAIAWVLLVIG
                     FLILAVTFGG"
     gene            complement(1780..2394)
                     /locus_tag="VGP27_09295"
     CDS             complement(1780..2394)
                     /locus_tag="VGP27_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7851543.1"
                     /translation="MRREVTTIDELRSIVGHPNAAAANKVGTRLSAEQQDWLAHCPLA
                     FVATTDAQGGVDVSPKGDPAGFVRVIDDTTIAIPERPGNKRVDGYLNVLARPHVGMLF
                     IIPGRGDTLRINGTARILADADYFDAMVVAGKRPILALEVDVEEVFFHCPKAFLRSDA
                     WNPDSWHPTAVPSVAQLARAIKKDMSLEELEKYYSEDHMRKILY"
     gene            2545..2889
                     /locus_tag="VGP27_09300"
     CDS             2545..2889
                     /locus_tag="VGP27_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3140 domain-containing protein"
                     /protein_id="HEV7851544.1"
                     /translation="MAKHVEVDPALWEEFHRVVNMTSRELMDWLRTRSASEESEEFAD
                     QAGSQTGRHVVHILGKRRVDLTADDERIMRKVVDRVHAERSVDMEPTAGDDAWRHRMM
                     TIGHDPLKPVRS"
     gene            complement(2904..>3167)
                     /locus_tag="VGP27_09305"
     CDS             complement(2904..>3167)
                     /locus_tag="VGP27_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851545.1"
                     /translation="VECQPNCAQGATLVNPIVVHAWNPVSSPSAACPQDVKFYSDLTI
                     AYPKGAPPWITPDTTWSPGTDFVTVDGMPAVHFSELSPTCEPL"
BASE COUNT          609 a          997 c         1044 g          517 t
ORIGIN      
        1 ctcggatctc agttcgtgcg ccgtgctgaa ccggtagcgg tacagcgtcg cccgtatctg
       61 gactggcgga gtgtcgggga atgggttggt ccgcaaaagc cggacgatcg agggttcgtt
      121 cgtcagcagc ccgcgacaca acggtatcaa ccaggtctgc gcgtaggccg gtgagatcgg
      181 caggaaccac atcagccagt cgagtcgcag gtgatacggg ctccactggc gcggcagtcg
      241 cttcacgtca cccggcttgc ctctgaattc gtattcggcc cagactgttt cgccggagac
      301 gtcggaggac gaggtgccct cgatgatcac ttcctcccgg gtgcggttga tgctgccgaa
      361 agcgccgtag gtgttgacca tgtgcagcgg gtcgtacgtg ctgttcatgc gttgtctgcg
      421 ggagatcagg ttgcgggccg gataccagct gcgcacgacc acgagcgcgg tgaaggcgat
      481 gacaagtgcc gcgtaccaat atggttgagt cgcagggata tccgtcggcg aagtaatgcc
      541 gagcatcgtc gtcagcgacg aggtgtcgat cacgctgacg gccagcacga tcgtcagcca
      601 gttcagccag gcgaagttgc ccgaaagcac cagccaaaac tgggtgaccg cgatgatcgt
      661 cgcggccgca ctggcgatcg gcgcaggtgc gaacaacccg aacgggacga tcagctgggc
      721 gaggtgattg cccgccgcct ccatccggtg aaagggccgc ggcaaatggt gaaagaacca
      781 gctgagcggc cccggcatcg gttgcgtttc gtggtggtag tcgagacagg tcaattggcg
      841 ccaacagcgg tcaccgcgca gcttgatcag gccggcgccg aactcgacgc ggaacagcag
      901 ccagcgggtc agccacatca cgagtgcggg tgggccgaca tcgtcgttgc cgacgaagac
      961 cactagcaga cctgcctcca gcagcagcga ttcccacccg aacgagtacc agatttgtcc
     1021 gacgttgacg atcgacaggt agagcaccca caacagcagc cacagcagac acgccgccca
     1081 taccggaacg gcgtcggcca ggcccgccgc tatggcggct gagattgtcg cacctaccca
     1141 gcagacggac gcgaagaaag cgtcggagta gtgcagatgg aacaggctcg gggtatttcg
     1201 aaatgaccga tgtgccagat agtcgggcac cggcagcatc ccgcgctcac cgatcaggcc
     1261 gcggaactgc cgagccgcgc acacgaacgc cacgacgtac acagcagcga cgccgcgctc
     1321 caaaaccagt ctgccccacc accattgcgg ggctatcagc caatccaccg gtcatgctcc
     1381 catgaggagc ctatcccccg aacgtgacgg cgaggatgag gaagccgata acgagcagaa
     1441 cccatgcgat cgccggtacc gcccacccgc gcggccgtcg cgccgtgaac gaggagatca
     1501 cgatggcggc cagcgccacc accggcgccc cgtatgtgat cagcccgtag aggaagtttc
     1561 ctggcccggt gtttgcgcag gcgcggtcgc tgcatcccgc ggtgcccatc acccgcatga
     1621 aggagtaggc ctcgatgagg agcgcgccga tgatggtcga cagcgcgagg ccccaattga
     1681 ccaccgtctg cttgcgcgac acccgcgcct gggattccgg cgtcgtcata gctggccaag
     1741 tacccgcaag tcaggtgccg aaacaccggt gcgacacgac tagtagagga tctttcgcat
     1801 gtggtcctcg ctgtaatact tctccagctc ctcgaggctc atgtccttct tgatcgccct
     1861 ggccagctgc gcgacgctgg gcaccgcggt cgggtgccag ctgtccggat tccaggcgtc
     1921 ggaccgcagg aatgccttgg ggcagtggaa gaacacttct tcgacgtcga cctccagcgc
     1981 cagaatggga cgcttgcctg ccacgaccat cgcgtcgaag tagtcggcgt cggcgagaat
     2041 gcgtgcggtt ccgttgatcc gcagcgtgtc tccccggccc ggaatgatga acagcatgcc
     2101 cacgtggggc cgcgcaagca cgttgagata gccgtccacc cgcttgtttc ccggccgttc
     2161 cgggatggcg atggtggtgt cgtcgatgac gcggacaaag ccagccgggt cacccttcgg
     2221 tgagacgtcg acgccaccct gcgcatcggt cgtcgccacg aaggccagcg gacagtgcgc
     2281 caaccagtcc tgctgctcgg cggaaagccg cgtgccgacc ttgttggcgg cagcagcgtt
     2341 cgggtggccg acgatcgagc gcaattcgtc gatggtggtg acttcgcgac gcatggctac
     2401 atcatgctcg actgcagttc atttctgcga cgccattaag tgcaacactg cgccgcggca
     2461 tcaccgacct cggtagcgcc ccttgcggcg gtgattagcc cctcgggcag gcgggtagcc
     2521 gatgggcgtt tggaggtaag tgtcatggcc aagcatgtcg aggtcgatcc ggcgctgtgg
     2581 gaggagttcc accgcgtcgt caacatgacg tcacgggaac tgatggactg gttgcgcacc
     2641 cggtcggcga gcgaggagtc cgaggagttc gccgatcagg ccggctcgca gaccggccgc
     2701 cacgtcgtcc acatactggg gaagcggcgc gtcgacctga cggccgacga cgagcgcatc
     2761 atgcgaaagg tcgtcgacag agttcacgcc gaacgcagcg tggacatgga gccgacggcc
     2821 ggcgacgacg cgtggcgaca ccgcatgatg acgatcgggc acgacccgct gaagccggtt
     2881 cgctcctagg gcccctcagc gcctcacagg ggttcgcacg tcggcgacaa ctccgagaag
     2941 tgcaccgcgg gcatcccgtc aacggtcacg aaatcggttc cgggagacca cgtggtgtcc
     3001 ggcgtgatcc atggcggcgc acccttgggg taggcgatcg tcaggtcgct gtagaacttg
     3061 acgtcctgcg ggcacgcggc gctcggtgac gacaccggat tccacgcatg cacgacgatg
     3121 gggttgacga gcgtcgcgcc ctgagcgcag ttgggttggc actcgac
//
LOCUS       DASYLX010000378         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3088961,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000378 DASYLX010000000
VERSION     DASYLX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088961"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGP27_09310"
     CDS             <1..180
                     /locus_tag="VGP27_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7851546.1"
                     /translation="SLSAGPLPSVGPLYKAKTIEMCVPNDPICFQGGRDMRAHGAYGQ
                     TGMVNDAAAFAASRL"
     gene            233..922
                     /locus_tag="VGP27_09315"
     CDS             233..922
                     /locus_tag="VGP27_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7851547.1"
                     /translation="MRIGKLIVATAIAWAALGGIPVPPASADPCPDVEVIFARGTGEP
                     PGVGGVGQAFVDALRSDAGGKSVAVYPVDYAASGDFNDTMAFAQTVVAGIRDAGAHVQ
                     STAANCPNTRIVLGGYSQGAAVAGFVTSAEVPQGVPAAFVPQPLPVEVADHVAAVALF
                     GKPSAAWTSQYGAPAITIGPAYAAKTIELCAQGDTICDGTPGGGPTFAHALYPVNGMV
                     GQAATFAVGRL"
     gene            complement(924..2138)
                     /locus_tag="VGP27_09320"
     CDS             complement(924..2138)
                     /locus_tag="VGP27_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851548.1"
                     /translation="MLGVEWPFNAKRQRRFDEGGTQRVRTVLGLSKTSMTIGWVLVDG
                     RDITGDPLDHDVFDISDSSAAAPAATARRVRDIATASGYTVDSVHVTTTGNVSSLRDA
                     LTESGFGEVVAVPLTEATRAWAIDAGRANEQATIAVCVLGRDSASLSVVDTDSGVLHA
                     TTTTITSDTVGLTDWLGTALGDNGSRPEALYLIGSRSKLRALAGPLGTGLSIPVVATH
                     DAQLALARGATISDGTRVHHGAVSRRSGLAAHARTLSVVAAVAVVSLFTLSSAGSPIP
                     LAKSSFPRSAPPAAGAEKVPTSAPPVAPAELPSPPAAAQSAPEEPTPTPTFEATPVVA
                     DVVAPDAVPAAAPATVDVAPQIEHVPEVQPVQHIPAQPAVAPDPAAPAPPDTPPPDPL
                     AAVLSPLFSGLP"
     gene            complement(2275..3579)
                     /locus_tag="VGP27_09325"
     CDS             complement(2275..3579)
                     /locus_tag="VGP27_09325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV7851549.1"
                     /translation="MASPTSVPDARTVAENTKPDRDRAVDVARLAALLVVMFGHCALL
                     LATIDSGGLRIGNLLGELPALTPITWVVQVMPLFFLAGGAAGAYGWREGTQWGTWLFT
                     RAQRLCRPVFWYLAFWTVALLVTAKIIDADSAAGLGGECVALLWFLGVYLVALAFVPT
                     LTRMRSGRAAIVVVGSLLAAAAAVDGVRIATGVPMSGVPNFLLVWLVPVVIGVAYARR
                     LIGVPAAFAVAVAAFAAQVLLATFGPYEVSLVVTGTERMSNVTPPTLLLALQCTWMSC
                     AFVALAGRVQRWAQRPRVWQAVAVGNGGAMTLYLWHIPAIAVATFCMHAVGLDAYDVT
                     ARGFWALLTLRAVIFAIVMAATFFLLSPLEHRRLLWWDSAVRATGVRSAAAGGFTCMA
                     GVALVMLAKNGLAGVTGYSLLGCFFVAAVAARISAGGPQREM"
     gene            3729..4040
                     /locus_tag="VGP27_09330"
     CDS             3729..4040
                     /locus_tag="VGP27_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7851550.1"
                     /translation="MALNRMALGSVAFAVALAAAPVASAAPRDVFLDELATLNIPLIA
                     GNQADTVGAGYHVCDQLRSGVSVLDEMTTAEQEFQLASGQGTLFVSAATTNLCPDFAA
                     G"
     gene            4174..4812
                     /locus_tag="VGP27_09335"
     CDS             4174..4812
                     /locus_tag="VGP27_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV7851551.1"
                     /translation="MGKGVLANGRGRLLVVVASLAVSAVMLYDPGSDSETPAAVATGL
                     VPSMSPAASPAAPDLVAAAAPVANQAASFALPVGVAPEAGLQIHTIWVARAISVMFPE
                     ITTIGGARQDALKWHPNGLAIDVMIPNHSSDEGIELGNQIAGLALANANRWGVIHVIW
                     RQGFYPGIGAPSWTADYGNETANHYDHVHIATDGGGYPTGHERYFIGSMTRA"
     gene            complement(5036..5182)
                     /locus_tag="VGP27_09340"
     CDS             complement(5036..5182)
                     /locus_tag="VGP27_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851552.1"
                     /translation="MIGLIDLTASTTSTLSRVKYAATHVGAMGVLFLAPATANATDND
                     SPRL"
     gene            5717..>5955
                     /locus_tag="VGP27_09345"
     CDS             5717..>5955
                     /locus_tag="VGP27_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851553.1"
                     /translation="MALPALEDIAARESSNDPIEGVIRIETSPREKATPVILDMMRSV
                     YPHEQVFGPYCTVNGYIDCPPDDLYAYLSDTRSLE"
BASE COUNT         1070 a         1938 c         1961 g          986 t
ORIGIN      
        1 agcctgtcgg ccggcccgct gccatcggtt ggtccgcttt acaaggcgaa gacgatcgag
       61 atgtgcgtac ccaacgaccc gatctgtttc cagggcggcc gggacatgcg ggcccacggt
      121 gcctatggtc agacgggaat ggtcaacgat gccgctgcct tcgccgcaag ccggctatag
      181 acgcagccga gttggggtat ccctcagcac aacgacgcgg ggaggatcat tgatgagaat
      241 cggcaagttg atcgtcgcga cagcgatcgc atgggcggca ctggggggca ttcccgtgcc
      301 accggcatcc gcagacccgt gcccggacgt tgaggtgatt ttcgcccgcg gcaccggtga
      361 gccgccgggg gtcggagggg tcggacaggc gttcgtcgac gcactgcggt cggatgctgg
      421 tggtaagtcg gttgcggtgt atccggtcga ctacgccgcc agcggcgact tcaacgacac
      481 gatggcgttc gcccagaccg tcgtcgcagg catcagggac gccggcgctc atgtgcagtc
      541 cacagcggcg aactgcccga atacccgtat cgtgctcggc ggctattcgc aaggcgcggc
      601 ggtggcagga ttcgtgacct ctgccgaggt gccgcagggg gttcccgcgg ccttcgtccc
      661 ccaaccgttg ccggtcgagg ttgccgacca tgtcgccgct gtcgccctgt tcggtaagcc
      721 atctgccgcg tggacgagcc agtacggcgc gccggcgatc acgatcggcc ccgcctacgc
      781 ggcgaagacc attgaactct gcgcccaggg cgacacaatc tgcgacggaa ctcccggcgg
      841 cgggccgaca ttcgcgcatg cgctttatcc ggtcaacggg atggtcggcc aggcagccac
      901 attcgcggtc ggccgcctct aattcacggc aaaccgctga acagggggct tagcaccgcg
      961 gccaacggat ctggcggagg cgtgtcgggt ggcgccggag cggcggggtc cggtgcaacc
     1021 gcgggttgtg ccgggatgtg ctgaaccggt tgcacttcgg gtacgtgctc tatctgcggc
     1081 gcgacgtcga ccgtcgccgg cgcggccgcg ggaacggcat cgggcgcaac gacatcggcg
     1141 acgaccggtg tggcctcgaa agtcggtgtg ggcgtcggct cttcgggcgc cgattgagcc
     1201 gcagcaggcg gcgacggcaa ctccgccggg gccaccggcg gggcgctggt cggcaccttc
     1261 tcggccccgg ccgccggcgg tgctgatcgt ggaaaactgc tcttcgcgag agggattggg
     1321 ctgcccgccg aagacaatgt gaacagagac accaccgcaa cagccgcgac cacactcagc
     1381 gtcctcgcgt gcgcggcgag gccggaacgc ctggagacgg cgccgtgatg gacgcgcgta
     1441 ccgtccgaga tggtcgcgcc acgggctagc gcgagctgcg cgtcatgcgt cgcgacgacc
     1501 ggaatcgaca gacccgtgcc gagcggaccg gcgagcgcac gcagcttgga tcgagacccg
     1561 atcaggtaca gcgcttccgg acgcgaaccg ttgtcgccca gcgcggtgcc gagccaatcg
     1621 gtcaggccca cagtgtcgga ggtaattgtg gtcgtcgtcg cgtgcagtac gccgctatcg
     1681 gtgtcgacca ccgacagcga cgccgaatcg cgcccgagca cgcataccgc gattgtcgcc
     1741 tgctcgttcg cccgtcctgc gtcgatcgcc cacgcacggg tggcctccgt caagggaacg
     1801 gccaccacct cgccgaaacc cgactccgtc agcgcatccc gaagcgacga cacgttgccg
     1861 gtcgtcgtca cgtgcaccga gtcgacggtg taaccgcttg ccgttgcgat gtcacgcacc
     1921 cggcgtgcgg tggcggcggg cgcggcggcg ctcgagtcgg agatgtcgaa aacgtcgtgg
     1981 tccagcggat cgcctgtgat gtctcgtccg tcgaccagca cccagccgat cgtcatcgac
     2041 gtcttcgaca gccccaatac tgttcgcact cgctgggtcc cgccctcgtc gaaccgacgt
     2101 tgccgctttg cattgaacgg ccactcgacg ccgagcatag ttcgagtgat tctgtgcggc
     2161 acggcaaccg tgcgagggtt taccaatctg cgacgctgac gtgaccgtca acgtgcacgt
     2221 tatccagccg acgttgcggt tcggtcaccg cacaacagcg tcatgtggaa caggtcacat
     2281 ttctctctgc ggcccgccgg cgctgatccg cgcagcgact gccgcgacga agaagcagcc
     2341 gagcagtgag tagccggtga caccggccaa cccgtttttg gccagcatga cgagcgcgac
     2401 accggccatg catgtgaacc cgcccgcagc ggccgatcgg acgcctgtgg cgcgaaccgc
     2461 ggagtcccac cagaggagtc gacgatgctc cagcggtgac agcaggaaga acgtggccgc
     2521 catgacgatg gcgaatatca ccgcccgcag tgtgagaaga gcccagaagc cccgtgccgt
     2581 cacgtcatac gcgtcgagac cgaccgcgtg catgcagaag gttgcgacgg cgatcgccgg
     2641 gatgtgccag aggtagaggg tcatcgctcc cccgttgccc accgcgacgg cctgccacac
     2701 ccgcggtcgc tgcgcccagc gctgcacgcg acccgcgaga gcaacgaacg cgcacgacat
     2761 ccaggtgcat tgcagggcaa gcaaaagcgt cggcggtgtc acgttggaca tgcgttccgt
     2821 cccggtgacg accagcgaca cctcgtacgg cccgaaggtg gccaacagca cctgagcggc
     2881 gaacgccgcc accgccaccg cgaacgcggc cgggacgccg atcagccggc gcgcataggc
     2941 gacaccgatc accacgggga cgagccagac gagcaagaag ttggggacgc ccgacatggg
     3001 cacgccggtg gcgatgcgca ctccgtcgac ggcggcggcc gccgcgagaa gcgacccgac
     3061 tacgacgatg gccgcgcgcc ccgatcgcat tcgcgtcagc gtcgggacga aggccagcgc
     3121 cacgaggtag acaccgagga accagagcag ggcaacacac tccccgccga ggcctgctgc
     3181 cgagtcggca tcaatgatct tcgccgtcac caacagggcg accgtccaga aagcgagata
     3241 ccagaacacg ggacggcaaa gtcgctgggc gcgggtgaac agccaggtcc cccactgcgt
     3301 accttcgcgc cagccgtacg cacccgcagc gccgcccgcc agaaagaaca gcggcatgac
     3361 ctgcacaacc caggtgatcg gagtgagcgc gggcagttcg cccagcaggt tgccgatccg
     3421 caggccgccc gaatcgatcg tcgcgagcag cagtgcgcag tggccgaaca tcacgacgag
     3481 gagcgctgcg agccgggcga cgtcgactgc gcggtcgcgg tcgggtttgg tgttctcggc
     3541 taccgtgcgg gcgtcgggga ctgacgtggg cgaagccatg cgtccagcat gccggtacgc
     3601 gggccggcac gggtgcgtag tactacgtga cggactgcat gtgtagggaa acaccggata
     3661 tctccccggc cgctcgtggc ttacgtttcg ggtgttcgtc gttcgtccca acatggaagg
     3721 acacctcgat ggctctcaac cgcatggcgc tgggatcggt cgcgttcgca gtggcgttgg
     3781 ccgccgcgcc cgtcgcctcg gctgcgcccc gcgatgtatt cctcgacgaa ctggcgacct
     3841 tgaacatccc tttgatcgcc ggcaaccagg cagacaccgt gggagcggga tatcacgtct
     3901 gcgaccagct tcgcagcgga gtttctgttc tcgacgagat gactaccgcc gagcaggagt
     3961 tccagcttgc ctcaggacaa ggcacccttt tcgtcagcgc cgccacaaca aatctctgcc
     4021 ccgactttgc agccggttag ctttccgggc tgacgctttc gcgaagtctc actctgtcgt
     4081 gcgggcgagt cgacatgccg acaacggcga gttgcaaaga cttacgaggc caagattgcc
     4141 acgctgcaat gggcgcggaa gtaagctgcg cccgtgggga agggcgtatt agccaacggt
     4201 cgtggccgct tgctggtggt cgtggcctcg ctcgcggtct cggccgtcat gctctacgac
     4261 ccgggttcgg attccgaaac tccggccgca gtcgcgaccg gcctggtgcc cagcatgtcg
     4321 ccggctgcaa gtccggcggc acccgatctg gtcgccgcag ccgcgcctgt cgccaatcag
     4381 gcagcgtcat tcgcattgcc cgtcggcgtt gctcccgagg cgggtttgca gatccacacc
     4441 atctgggtgg cccgcgcaat cagcgtgatg ttccccgaga tcaccaccat cggcggggct
     4501 cgacaagacg cgttgaaatg gcatcccaac ggattggcga tcgacgtgat gattccgaat
     4561 cacagcagcg acgagggaat cgaactcggt aatcagattg ccggcctggc actcgccaac
     4621 gccaatcgct ggggcgtgat ccacgtgatc tggcgccagg gcttctatcc tggcatcggc
     4681 gcacctagtt ggacggccga ctacggcaac gagaccgcca accactacga ccacgttcac
     4741 atcgccaccg acggcggcgg atacccgacc gggcacgaga ggtacttcat cgggtcgatg
     4801 actcgcgcct gaaatgtcgg cccccctgtt ggatggcggg cccactgatg gggaaacggt
     4861 gatcagggtt gtgttaacac aggcaggttt gcctgataac gggcggccgc accgcgtctt
     4921 ctccggcgcg gtggtgacga tgatctttcc gccgtggtag ctgacgatct tcccgtcgac
     4981 gacgacatcc tgaccgtcgt tgattggtgt agccgtcggc gtcggtgtgc acccgtcaca
     5041 gcctgggcga gtcattgtcg gtggcgttgg cggtagccgg ggcgaggaag agaacgccca
     5101 tggcgccgac atgcgtcgcg gcgtacttga cgcgcgacag cgtgctggtg gtcgatgcgg
     5161 tcagatcgat gagtccgatc atgtgtacgt ggcgtggact tgtcgaggaa ctgcttcggt
     5221 ttcgcggggc atccgtactc gcccacgagc cacaaatcga cgtccgttgg gcagagggtt
     5281 tttgtccgtc aggaaagcgc cacgccgaga acggatttgg taaccccgac gacaatagtc
     5341 cgctcgctga cacttctagc taattcccat cggcgcaggt cgaagacggc gccgccgtcg
     5401 agtcacctct ctctgttcga gtagttgcgc gtgcaagtac agcgcctggc cagcctttgc
     5461 ggtgaggtgc cacaccacgt gactgcctga gccgcgccgg tcggccgtcc ggccgccgcc
     5521 cggatagtcg gtggactaga ccgcgcatca actagccagg ctaaatccgt gtaagcctgt
     5581 gttacatcct cgacacgccc ttgccaacac aagtcgaggt aaattcgcag attttttcca
     5641 gcattgacaa tttgctgctt atgatcccgg ttgtcggtct aagctcggcg gacggacaac
     5701 tacgcagggg gacaccatgg cactgccagc gcttgaagat atagcggctc gcgaatccag
     5761 caacgatccg atcgagggtg tgattcggat tgaaacctcg ccgcgggaga aggccacgcc
     5821 ggtcatcttg gacatgatgc ggtcggtgta tccgcatgag caggtgttcg gcccgtactg
     5881 cacggtcaac ggctacatcg actgcccgcc cgatgacctc tatgcctacc tttccgatac
     5941 ccgcagcctg gagga
//
LOCUS       DASYLX010000379         5144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1113907,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000379 DASYLX010000000
VERSION     DASYLX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113907"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGP27_09350"
     CDS             <1..484
                     /locus_tag="VGP27_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7851554.1"
                     /translation="MFELWHSDTPSFDGEFVKFDDIAFGPKPIREPHPVIWMGGDADA
                     VLRRAARFGDGWAPWLTKPDELPAKMDYLRSQPGFDDRPFSLFYSLAVLSIGEEHVAV
                     DDPGAQFGQSAQQVIDNCGMLAERGVTDTWVNPPPLDDFNAYLDHMQWVAEEVIPKVD
                     "
     gene            complement(524..634)
                     /locus_tag="VGP27_09355"
                     /pseudo
     CDS             complement(524..634)
                     /locus_tag="VGP27_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354640.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nonspecific lipid-transfer protein"
     gene            complement(690..938)
                     /locus_tag="VGP27_09360"
     CDS             complement(690..938)
                     /locus_tag="VGP27_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851555.1"
                     /translation="MESTAARTVFPETPDTPMIASIDDPSAEGRCLRPLLRAAESWCQ
                     VFSEPGAGSDLSSLSTRAVRDKDVYRMINAFSARPLAL"
     gene            complement(884..1537)
                     /locus_tag="VGP27_09365"
     CDS             complement(884..1537)
                     /locus_tag="VGP27_09365"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851556.1"
                     /translation="MRLQLRRGVGPMSEAAAGDARVSRTRAAVADAVASLFEEEGASG
                     LTHQRVATRAGVGRATVYRHWPKTVDLLTEALMGADQPLLHLGDGPLRQWLHSELIQA
                     AEQLAQPVSSQVIAMIVGTADQSPAARVLRENLNRRTRIVVDHMLDQATANGELSSRP
                     DTDELVATVFGAIMFRITIQHENVDSGYLARLVDGALAEHWSRPPPAPCFPKHPTPP"
     gene            4162..5055
                     /locus_tag="VGP27_09370"
     CDS             4162..5055
                     /locus_tag="VGP27_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851557.1"
                     /translation="MTTRVNYDRPQNVLSSGSSKVGQSRLKAYPLRTARHVGMIPRRT
                     AATTGRGVQLFADVIRYVVADAITMRLAVGDVFVQAWTLLKVTTIPALLMAVPIGAIT
                     TIVTSGLVNQLGATSLLGAAAGVGVIRQGAPLTAGLLMGAAAASTIAADFGARAIREE
                     LDAMRVMGVDPVRQLVVPRFLALLLIAPILCIFIVASGTASSFLMAVGASDVAPGSFW
                     MSFGTFTKVVDVWFAVGKTVVFGAIVAIVSSLRGMEAKGGPRGVADAVNSSVVVNVVL
                     IVFANLAITQLQSMFVPMEVA"
BASE COUNT          954 a         1615 c         1579 g          996 t
ORIGIN      
        1 catgttcgag ctgtggcaca gtgacacacc gagttttgac ggcgagttcg tgaagttcga
       61 tgacatcgca ttcggcccca agccgatccg cgaaccacat cccgtcatct ggatgggcgg
      121 cgacgccgac gcggtgctgc gccgcgccgc gcgcttcggt gacggctggg cgccctggct
      181 gacgaagccc gacgagttgc cagcgaaaat ggactacctc cgctcccagc caggattcga
      241 cgaccgtccg ttctcgctgt tctacagcct cgcagtgctg tccatcggcg aggagcacgt
      301 cgccgtcgac gacccaggcg cgcaattcgg tcagagcgcc cagcaggtga tcgacaactg
      361 cggcatgctg gccgagcgcg gtgtcaccga cacctgggtc aacccgccac ccctcgacga
      421 cttcaacgcc tacctcgacc acatgcagtg ggtcgccgag gaagtcattc ccaaagtcga
      481 ttagccaacc tgctgagcag gtcgactgtg ccgtttcgta tgccaaggcc gccaccgcgc
      541 catccacctc cgacgtacag tccagcgccc ggaacggttc gttgatccag cgggcggcca
      601 ggtagtcgtc catggtgaga ggttcacggc gcagccgtgc tctgcaacgc ctccttcggc
      661 gacggcctac caaagatgac agttcactgt catagtgcca acggccgagc ggagaatgcg
      721 tttatcatcc ggtagacgtc cttgtcgcgg acggcgcggg tggacagcga cgagaggtcg
      781 ctgcccgctc cgggttcgga gaacacctgg caccagcttt cggcggctcg tagcagcggc
      841 cgaaggcagc ggccttctgc cgagggatca tcgatcgagg cgatcatggg ggtgtcgggt
      901 gtttcgggaa acacggtgcg ggcggcggtc gactccaatg ttcggcgagc gcgccatcga
      961 caagcctggc gaggtagccc gaatcgacat tctcgtgctg gatagtgatg cggaacatga
     1021 tggcgccgaa cacagtcgcg accagttcgt cggtgtcggg tcggctgctg agctcgccgt
     1081 tggcggtcgc ttggtcgagc atgtggtcga cgactatgcg ggttcggcgg ttcaagttct
     1141 cacgcaagac tcgcgcggcg gggctttgat cggcggttcc cacgatcata gcgatgacct
     1201 gagacgaaac cggttgagcg agctgctcgg ctgcttggat gagttcgctg tgcagccatt
     1261 ggcgaagcgg gccatcgccc aaatgcaata acggctggtc agcccccatc agcgcttcag
     1321 tcaacagatc cactgtcttg ggccagtgcc gatacacggt ggcgcgcccc acacccgcgc
     1381 gcgttgcgac acgttgatgg gtcaaccccg acgcgccttc ctcttcgaac agcgatgcga
     1441 ccgcatctgc cacagccgca cgagtgcggc tcacccgcgc atccccggcc gctgcttcgc
     1501 tcatcggccc tacaccccgt cgcagttgaa gtctcatgac atccagcaaa gcaagtctaa
     1561 tgttgcgggg atgtcctgac gtctcgcgaa ctccgaacca tccgagtttt acgcaccctg
     1621 atggcgactc cggttcaatg acgcttccgg tcagcggcta cgttgaccag ggctgcgacg
     1681 gtcactcgac cgccgtgcta tatcggtgat tgctcgttcc gccgacggta acgcagctct
     1741 cggtagcgct cgttgtggat ctcggtacat ttcggacaac gacagcccca atatgaatag
     1801 ccgctgaccg tgccgtgcgc gtcgactggt gtgggctcgc tgcgcttccg cccgcgccgc
     1861 ttccgaaccg tgaatgccca ggctcgccga cagtgctggc agcgacaacg cagattggaa
     1921 tagccgtttt cagtgccatg ccgtggatca ccatcgcccg gatggatgat aggagtgggc
     1981 cgctctgata tgtccaccgt cgaacctccc atagtgtcga cctgtgcgaa tccgccaacc
     2041 ttcctggcgc gtcccaccag gtgaacggtg ttgggcgaca tcacaagccg ttcgccgagc
     2101 ctgtcggctc gcgcgccccg cgagttcctg ccctctgcgg ccgtataacc aacccgtcga
     2161 gccgcgatca ctcggctgtc gagggattgg ggacgttcat ggtctgtgcg cgcggagagc
     2221 agaatggcgg tcaggtcttt gaaatcccgc tgtcgttgtc gtcgttcgac gtatccgtcg
     2281 atgagtcgca gggggaacat gcgtcggcct ccgacttcgc ggctgggccg tcgattcgaa
     2341 gagtcggcgg tcctcaatcc cctaccgccc tcgccggaag gcatcgccgc gttgtgcgtt
     2401 ggccatcgca ggtgtcgcca cctgctcggc gaaggagcca tcgcgtcccg cccggtgggg
     2461 cgtgcgaccg cccgagccac ccacggtcgt caaccaggta tcgccgttcc gatagtggaa
     2521 caacgcgtag ccactcggcc gctgcggccc cggaacgtac tcgaacgccg atcacacgct
     2581 ggggctggcc gcgatcaact cggcggcatc gtggtgatct gtgaccgtca tatcaaggat
     2641 gtcctgatct cgttgcggca ctgcggcccc cggagacggg cgaatcgcgg tgaaccacaa
     2701 cgccgagcgg gggttggaag acttctttgt ccgcaaagct catgtccgtt gaacgatgca
     2761 cgtgttgaat cttcgtccaa ggccgtgacc ccggattcga caaagcctgc tacgaaccga
     2821 gcaatgagct gctccacaga atgcggtgaa aatactgggc cgggagggtt ccatgacgac
     2881 cctcggcgtc catgggtggc ttgccccgct ccacggtcat gaccgtgtcg tagaaatccg
     2941 aaggcgccct tgtagcttga gactcggctg cggtgtcccc ccagaaccgc cgaattgtcg
     3001 aagccgcgca ttaacgcaca ccaccgcgga tcgtggaaag caggtcggtg gaactggccc
     3061 cacgggaggg acgtacgaag accgacgtcg tcattcgtgc atacctctca agggtgggcg
     3121 actacaggca gagagcttcc taaacatccg aaaagagttt gctggcgctc gccacattga
     3181 ccgcacgacg gcctaacgac gtctttggtc gtcacccatg cgaatctcat gtggaccggc
     3241 gcagcgaagt tcagatccgc agaaacggat ccccatgcgg gtcgcgtgga tggcggttgg
     3301 accctcgcaa aaccccgcca caccgaacgc caactcccat ccaacgctga agaccaccta
     3361 tcggaatccg gtgatagcga acaaacacag tgtcgaccag attcagggcc gtgccgtctt
     3421 cgtgccgcgc gcctagctgg gccgagcata gacacgcata catcactaac atcaaaatga
     3481 cagtagccga acacatctca ttgacacgac tgcacccacg gctgccttag atcccgattc
     3541 ctgtcaagct gtcaaagcca cggcggacac ccgcgtcttg atgctggcaa cctcgcgcct
     3601 cctcacccga aattctgctg tgagacatca tgcaccacat ctatctgtgg gtcaagttgt
     3661 ctcatggcaa cacgaccggt tggctgggac agctggcgca cgccagcatt tccaacgcgt
     3721 caacgcgcac gcttgccaag cggcagatcg ccctctacgg taatccgaca aatgtttatc
     3781 gggtttgctc gcggggtcat acttggagtg ctcggcagcg cagcgccgcg aaactgccgg
     3841 caagagagga cgtttgatgg agccgtggcc tcagatcgga tccagtgctg aaaggtccgg
     3901 cgcaccaggc gatctcggcc tccccaccca cgttcggcgc ctggggtggg gggtcggcac
     3961 ttgggccacc tcagacccat cgtgcagggc caacgagggc ggtaatactg acgcgtcgcg
     4021 acgatttggc catactgcgc ggtctggaga tccactcctc gccaacactt ttggaatgct
     4081 ggctacaatg ctgccgctaa tatctatggt cgttgatacg ccgcagcgca cgcgtcccca
     4141 caagcgttct aacggtggtt catgaccact cgtgtgaact acgatcgacc acaaaacgta
     4201 ttgagttcag gctccagcaa ggtgggccaa tcacgcctca aggcttatcc gcttcggact
     4261 gcgcggcacg taggcatgat tccgaggcgt accgccgcca cgaccggtcg tggtgtccaa
     4321 ttgtttgcgg atgtgattcg ctatgtggta gccgatgcca tcaccatgag actcgcggtc
     4381 ggcgacgtgt tcgtgcaggc ctggaccctg ctcaaggtga cgaccatacc cgcgttgctg
     4441 atggccgttc cgatcggcgc gatcaccacc atcgtgacgt ccgggcttgt caatcaactg
     4501 ggggcgactt cgctgctcgg cgcggccgcc ggggtgggcg tgattcggca gggagcgccg
     4561 ctgacagccg gactgctgat gggtgccgca gcggcttcga caatcgcggc ggacttcggg
     4621 gcacgcgcca tccgtgaaga actcgacgca atgcgagtaa tgggtgttga cccggttcgc
     4681 cagttggtcg tgccgcgctt tctcgccctg ctcttgatcg ccccgatcct gtgcatcttc
     4741 atcgtggcgt cgggaactgc gtcgtcattc ttgatggcag tgggagcctc cgacgtggca
     4801 ccaggaagtt tctggatgtc gttcggcacg ttcacgaagg tggtggacgt atggttcgcc
     4861 gtcggtaaga cggtcgtctt cggggcgatc gtggcgatcg tttcgtcgct tcgtggaatg
     4921 gaggccaagg gtggcccgcg gggtgtcgca gatgccgtga actcatcggt tgtggtcaat
     4981 gtcgtcttga tcgtcttcgc aaacctcgcc ataacgcagt tgcagtcgat gttcgtgccg
     5041 atggaggtcg catagtgact tctggtagcc gcgccgtggt accgagcacg tacggcacgc
     5101 gaatggtaag agcgacagtg gatccgatcg ccacgatatt cctc
//
LOCUS       DASYLX010000380         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_17401, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000380 DASYLX010000000
VERSION     DASYLX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17401"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGP27_09375"
     CDS             complement(<1..297)
                     /locus_tag="VGP27_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis bifunctional protein
                     CbiET"
                     /protein_id="HEV7851558.1"
                     /translation="MIVVIGIGADGMAGLARTSRDELRRAAVIFGSQRQLDLLDDTVE
                     AVRRPWPSPMLPGLRTLLDGIDGDVHVVASGDPLLHGVGATLIRMYGADKVAVLP"
     gene            complement(294..1043)
                     /locus_tag="VGP27_09380"
     CDS             complement(294..1043)
                     /locus_tag="VGP27_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851559.1"
                     /translation="MDDTSRGPVVIFGGRSEIGVELAQLLAPDATVVLAARNPDRLSE
                     QVAAIEAAGAAKVDVREFDADDLASHGPLVTSIVADYGAIGTAVLAFGILGDQTRAEK
                     DPAHAAAVLHTDFVAQASLLTVLSDTMRTAGSGAIVVFSSVAGVRVRRANYVYGSAKA
                     GLDGFCSGLADALHGSGVRLLLVRPGFVIGRMTEGMKPAPMSSTPPQVAAAAARALAK
                     GRSTVWIPWMLRPLFVGMRLLPQRVWRRLPR"
     gene            1079..1492
                     /locus_tag="VGP27_09385"
     CDS             1079..1492
                     /locus_tag="VGP27_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent biliverdin reductase"
                     /protein_id="HEV7851560.1"
                     /translation="MANATTRLTNDALAFLTERHLAMLTTLRSDNSPHVVAVGFTFDP
                     KTHIARVITSGGSQKAVNAEHRGVAVLSQVDGARWLSLEGRATVNTEAEAVRDAELRY
                     AQRYRTPRVNPRRVVIEVRIERVLGSSELLDRTGD"
     gene            1549..2958
                     /locus_tag="VGP27_09390"
     CDS             1549..2958
                     /locus_tag="VGP27_09390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEV7851561.1"
                     /translation="MSSEQVVKRIAAVSEAFDDLLSEPLDGLSPSELAAAAAAWVTLV
                     RRLPVLDHRIVAALSRVPPAELGEKTVKRALCTLLRISGKEAKRLISEATNLAPRATL
                     TGEPMPPLMSATAAAQARGAISAEHVKIIAAFVKELPSWVDIDTREHAEVDLAEVAAH
                     HDPDGLQKAADRLAYMIDQDGPEPVDAELNCKGTVTIGRQRPNGLSPVHGEVDPEGRA
                     VWEAIFATEAAPGACNPDDENPCVDGKPDEQHVTSDTRTPGQRRHDAFKAVGRAILAS
                     GRLGQHKGLPITVIVSTTLKELESGAGHGVTGGGSLLPMRDLIRLASQAHHYLTVFDD
                     HTGVPLALARAKRLATPGQRIVLHARDHGCTRPGCTVPGYWCEANHDDPWSTGGHTDV
                     STMSFTCGPDNRLLHETGWTVCKRKDGRTEWIPPPQLDTGQARINFYHHPERYLVPDE
                     HATERGGDDDELPLAGSHP"
     gene            complement(2965..3768)
                     /locus_tag="VGP27_09395"
     CDS             complement(2965..3768)
                     /locus_tag="VGP27_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7851562.1"
                     /translation="MSGWRPDPTARHEGRYYVAGRPTSRVRSGKAETTDPDGGKLLPA
                     YLDMPSRSRTSIRSTWLTTAAVTVVLVMSAAVIWAVLLLPRHNPGSIDADFLSALKDS
                     GFADQFNSDANALAHGRQVCKQLEGGGPQQGVAADKLAVDAFCPRFSEGFRILETATA
                     QGTFVLMDSSKAYVSSIASDEGKCHGVRGYSDIGSGTQVMVKNGKGEIIASTSLGEGH
                     GDDVNCRFAFSFPVTDGQDRYVVSVGHRGEFSYNFEQLRRGGVEIHLGE"
     gene            complement(3865..>4726)
                     /locus_tag="VGP27_09400"
     CDS             complement(3865..>4726)
                     /locus_tag="VGP27_09400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV7851563.1"
                     /translation="DPYRAVADALAGAPASGGSALAVAVTDSMPALHLLPLADVLGVV
                     PVLATDVLRRLRMVKDGAEIDALRKAGAAIDRVHARVPEFLVPGRTEADVAADIAEAI
                     VAEGHSEVAFIIVGSGPHGADPHHECSDRELRVGDIVVVDIGGPYEPGYNSDSTRTYS
                     IGEPSPEVAQHYSVLQRAQQAAVEAVRPGITAEQVDAVARDVLAEAGLAEAFVHRTGH
                     GIGLSVHEEPYIVAGNDLPLAEGMAFSVEPGIYFPGQWGARIEDIVVVTADGAESVNA
                     RPHDLIVVRT"
BASE COUNT          840 a         1641 c         1499 g          746 t
ORIGIN      
        1 cggaagcacc gcaaccttgt cggcgccata catccggatc agggtggcgc cgacaccgtg
       61 cagcaacggg tcgccgctgg ccaccacgtg cacgtcaccg tcgatgccgt cgagcagcgt
      121 acgaaggccg ggcagcatcg gcgacggcca cggccgcctg accgcctcga cagtgtcgtc
      181 gagcagatcg agttgccgct gggatccgaa gatgactgcg gcgcggcgca attcgtcccg
      241 cgatgttcgc gccaaaccgg ccatcccgtc ggcgccgata ccgatgacga cgatcaccgc
      301 ggcaacctcc gccacacccg ctgcggcagc agccgcatcc cgacgaagag cggccgcagc
      361 atccacggaa tccagacggt gctgcggcct ttcgccagcg ccctcgcggc cgccgcggca
      421 acctgcggcg gcgtactcga catgggcgcc ggtttcatgc cctcggtcat ccgcccgatc
      481 acgaagcccg gccgcacgag cagtagccgc acccctgaac cgtgcagggc gtcggcaagg
      541 ccgctgcaga aaccgtccag tccggccttc gcggagccgt agacatagtt ggcgcgccgc
      601 acccgcacgc cggccaccga ggagaacacg acgatcgcac ccgacccggc agtcctcatg
      661 gtgtcggaca gtacggtcaa caggctcgcc tgggcgacga agtcggtgtg cagcaccgcc
      721 gcggcatgtg cggggtcctt ctcagcacgg gtttggtcgc cgagtatccc gaacgccagc
      781 accgcggtgc cgatcgctcc gtaatcggcc acgatcgagg tcaccagtgg tccgtgcgag
      841 gcgaggtcgt cggcgtcgaa ctcacggacg tcgaccttgg ccgctccggc cgcctcgatg
      901 gcggcgacct gctcggacag ccggtcggga ttgcgggcgg ccaacaccac ggttgcgtcc
      961 ggtgccagca gctgggcgag ttcgacgccg atctcgcttc ggccgccgaa aatgaccact
     1021 ggtccgcggc tcgtgtcatc cacggctgcg attatctcct gcgctagttt ggaggccgat
     1081 ggcaaacgca acgacccggc tcaccaacga cgcgctggcg ttcctcaccg agcgccatct
     1141 cgcgatgctg accaccttgc gatcggacaa ttcgccgcat gtcgtcgcgg tcgggttcac
     1201 attcgatccg aagactcaca tcgccagggt catcaccagt gggggctcgc aaaaggcggt
     1261 caacgccgag caccgcggcg tcgccgtcct cagtcaggtc gatggcgccc gatggctgtc
     1321 gctggagggc cgcgccacgg tcaatacgga ggccgaggcg gtccgggacg ccgaactgcg
     1381 ctatgcgcag cgctaccgca ccccgcgagt gaatccgcgg cgggttgtga tcgaagtccg
     1441 gatcgagcgg gtactcggct cgtccgaact gctcgaccgc accggcgact gacattccgt
     1501 cttcaatccc taccgtcgat gtcggtggtt cgaactagtg ttcgaagcat gagttccgag
     1561 caggtcgtaa agcgcatcgc cgcagtgagc gaagcgttcg atgacctgtt gtcggaaccg
     1621 ctggacgggt tgagcccttc ggagctggcg gcggccgccg cggcctgggt aacgctggtc
     1681 cggcgcttac cggttctcga ccaccggatc gtggccgccc tgagccgggt ccctcccgcg
     1741 gagttgggcg agaagaccgt gaaacgggca ctgtgcacgt tattgcggat ctccggtaaa
     1801 gaggccaaac ggctgatcag tgaagccacg aatttggctc cgcgggccac tttgaccggt
     1861 gagccgatgc cgccgctgat gagcgcgaca gccgcagctc aggctcgcgg cgctatcagc
     1921 gccgaacatg tcaagatcat cgccgcgttc gtcaaagagc tgccgagttg ggttgatatc
     1981 gacacccgtg agcacgccga ggttgatctg gccgaggtcg ccgcacacca tgaccccgac
     2041 gggttacaga aggctgccga ccggctggcc tacatgatcg atcaggacgg acctgaaccc
     2101 gtcgacgccg aattgaactg caaaggcacc gtcacaatcg gcaggcaacg gcccaacggc
     2161 ttgagcccag tccatgggga agtcgatcct gagggccgcg cggtgtggga ggccattttt
     2221 gccaccgagg ccgctcccgg agcgtgcaac cccgacgacg agaacccttg cgtcgacggg
     2281 aaacccgacg agcagcacgt cacctctgac acgcgcacac ccggccaacg tcgccatgac
     2341 gcattcaagg ccgtgggcag ggcgattctc gcctccggac ggttgggaca gcacaaaggc
     2401 ctacccatca ccgtcatcgt ctcaacaacc ctcaaggaat tggaatccgg cgcaggacac
     2461 ggcgtcaccg gcggcggatc cttgctgccg atgcgcgacc tgatccggct ggcttcccag
     2521 gcacatcact acctgacagt gttcgacgac cataccggcg tgccgttggc gctggcccgt
     2581 gccaaacgcc tcgcaacccc cggccaacgc atcgtcctgc acgcccgcga ccacggttgc
     2641 acccggccgg gctgcactgt tcccggctac tggtgtgaag ccaaccacga cgacccctgg
     2701 tctaccggcg gtcacaccga tgtcagcacg atgtcgttca cctgcggacc cgacaaccgg
     2761 ctgctgcacg aaaccggctg gaccgtctgc aagcgcaaag acggtcgcac cgaatggatc
     2821 ccaccaccac aactcgacac cggccaagcg cgtatcaact tctatcacca ccctgagcgc
     2881 tacctcgttc ccgacgaaca cgccactgaa aggggcgggg acgacgacga gctcccgttg
     2941 gcgggcagcc atccgtaggc cacactattc gccgaggtga atctcgacgc cgcctcgccg
     3001 cagctgttcg aagttgtagc tgaattcgcc gcgatgaccg acggagacca cataacggtc
     3061 ctggccgtcg gtgaccggga agctgaaggc aaacctgcaa ttgacgtcgt cgccgtgacc
     3121 ctcgccgagt gacgtggagg cgatgatctc gcctttgccg ttcttcacca tgacctgggt
     3181 gccgctgccg atatcggaat agccgcgtac tccgtggcat ttgccttcgt cggacgcgat
     3241 cgagctgacg taggccttcg atgagtccat caacacgaaa gtcccttgcg cggttgctgt
     3301 ttcgaggata cggaaaccct ccgaaaatct cgggcagaag gcgtcgacgg cgagcttgtc
     3361 ggcggccacc ccctgctgcg gcccgccgcc ctcgagttgc ttgcacacct gcctgccatg
     3421 ggccaacgcg ttcgcatcgg agttgaactg gtcggcgaat ccggagtcct taagggccga
     3481 caggaaatcg gcgtcgatcg acccggggtt gtgacgcggc aacaacagaa cggcccagat
     3541 gaccgccgcc gacatcacca gcacaacggt cacggcagca gtggtcagcc acgtcgatcg
     3601 aatgctcgtc ctgctcctgg acggcatgtc gagataggcc gggagcagct ttccgccgtc
     3661 ggggtcggtc gtttcggcct tcccgctacg cactcggctg gtgggacggc ctgcgacgta
     3721 gtagcggcct tcgtggcgtg cggtcgggtc gggtcgccat ccgctcatgg cgacaccctc
     3781 cttgagcgcg ccgcagttcg ggcagacgtc ggttcttccg acgtcggaac cgcagaacgg
     3841 gcaggacatc tccgggccca gtagctaggt ccgcaccacg atcaggtcgt gagggcgcgc
     3901 gttcacggat tcggcaccgt cggcggtgac gacgacgatg tcctcgatgc gcgcacccca
     3961 ctgacccggg aagtagatgc ccggttcgac ggagaatgcc atgccctcgg ccaacggcag
     4021 gtcgttaccc gccacgatat agggctcctc gtgtaccgac agcccgatgc cgtggccggt
     4081 gcggtgcacg aacgcctcgg cgagaccggc ctcggccagc acgtctcggg cgaccgcatc
     4141 cacctgttcc gcggtgatcc ccggccgcac ggcctcgacg gcggcctgtt gcgcacgttg
     4201 cagcaccgaa tagtgctgcg ccacttcggg gctgggctcc ccgatgctgt acgtcctcgt
     4261 cgaatcggag ttgtacccgg gctcgtacgg ccccccgatg tcgacgacga cgatgtctcc
     4321 gaccctcagc tcacggtcgg agcactcgtg gtgagggtcg gcgccgtgcg ggccggatcc
     4381 gacgatgatg aaagccacct ccgaatgtcc ctcggcgaca atggcttcgg cgatatcggc
     4441 ggccacgtcg gcctccgtac ggcccggcac aaggaactcc ggcaccctgg cgtgcacccg
     4501 gtcgatcgcc gctcccgcct tacgcagcgc gtcgatctcc gcgccgtcct tgaccatccg
     4561 cagccggcgc agcacatcgg tcgcgaggac cggcaccacg cccaacacgt cggccagcgg
     4621 cagcaggtgc aacgcgggca tcgagtcggt gactgcgacg gcgagagcgc tccccccgct
     4681 cgcgggcgca ccggcgagcg catcggccac cgcccgatac gggtcg
//
LOCUS       DASYLX010000381         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_345760,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000381 DASYLX010000000
VERSION     DASYLX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345760"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1738
                     /gene="lysX"
                     /locus_tag="VGP27_09405"
     CDS             18..>1738
                     /gene="lysX"
                     /locus_tag="VGP27_09405"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HEV7851564.1"
                     /translation="MTLTSPPLVRPTSRYSWVPAAAGWTVGVIATLSLIASLSPGIRW
                     LIKEPREFVNDYIFNFPDTSFAWAFVLALLGAALAARKRLAWLILVLYMVGSIGWNIV
                     DIAGRGETWMRETGELIGLAFHIAAVVFLVLARKEFWAKVRRGALFKAAVTLVAGMAI
                     GILVGWGLLELFPGTLERDYRFAYAANRVVAFAGVGPNAFDGQHPHVFVNALLGLFGA
                     LALMAAAVVLFRSQRSENALTGEDESAIRGLLELYGKNDSLGYFATRRDKAAVFAPNG
                     RAAITYRVEVGVCLASGDPIGEPRSWPQAIEAWLRLCQAYGWAPGVMGASLDGAEAFR
                     AAGLNALQLGDEAILQPDGFRLSGPDMRAVRQAVTRARRAGLTVRTRRHRDIAPDEMA
                     EVIRRADAWRDTDDERGFSMALGRLGDSADGDCLLVEAVQQQQNVVAMLSLVPWGNNG
                     VSLDLMRRSPHSPNGTIELMVTELLQQSEGIGITRVSLNFAMFRSAFEQGAQLGAGPV
                     ARLWRALLVFFSRWWQLETLYRSNMKYKPQWVPRYACYEDARLVPRVGVASVIAEGFL
                     VLPFSRR"
BASE COUNT          255 a          569 c          588 g          326 t
ORIGIN      
        1 gaatatcctc gcggcctatg accctcacta gcccgccgct ggtacggccc acctccagat
       61 actcgtgggt tcccgcggcc gcaggctgga ccgtcggcgt catcgcgacc ttgtcgctga
      121 tcgcgagcct ctcaccgggc atccggtggc tcatcaaaga accgcgcgaa ttcgtcaacg
      181 actacatctt caacttcccc gacaccagct tcgcctgggc attcgtgctg gccttgctgg
      241 gcgcggcgtt ggcggcacgc aaacggctcg cctggttgat cctcgtcctc tacatggtcg
      301 gctcgatcgg ctggaacatc gtcgacatcg cgggccgcgg cgaaacctgg atgcgcgaaa
      361 ccggcgagtt gatcggcctc gcattccaca tcgccgcggt cgtcttcctg gtactggccc
      421 gcaaggagtt ctgggccaag gtgcggcggg gtgccctgtt caaggctgct gtcacgcttg
      481 tcgccgggat ggccattggc atccttgtcg gctgggggct gttggagctg ttccccggca
      541 cgttggagcg cgactatcgc ttcgcctatg ccgccaaccg ggtggtggcc ttcgccgggg
      601 tgggtcccaa cgcgttcgac ggccagcacc cgcatgtctt tgtcaacgcc ctgctcggtc
      661 tgttcggcgc gctggcgctg atggccgccg cggtggtgtt gttccgttcg cagcggtccg
      721 agaacgcgct gacgggtgag gacgaatcgg cgatccgagg gctgctggag ctctacggca
      781 agaacgactc cctcggctac ttcgcgaccc gtcgggacaa ggccgcggtg ttcgcaccca
      841 atggacgtgc ggcgatcacc taccgcgtcg aggtcggcgt gtgcctggcc agcggcgacc
      901 ccattggcga gcccagatcg tggccccagg cgattgaagc ctggctgaga ctgtgccagg
      961 cgtacggctg ggcgcccggc gtgatgggtg ccagtctgga tggcgctgag gcgtttcgcg
     1021 cggcggggct caacgcgctt caactcggcg atgaagcgat cctgcaaccc gacggcttcc
     1081 ggctctcggg tcccgacatg cgcgcggtcc gccaggcggt cacgcgggcg cggcgcgccg
     1141 ggctgacggt gcgtacgcgg cgacaccgcg acatcgcccc cgacgagatg gccgaggtca
     1201 tcaggcgggc cgacgcatgg cgcgacacag atgacgagcg cggcttctcg atggcgcttg
     1261 gccggctcgg tgattccgcc gacggcgact gcctgctggt cgaagcggta cagcaacagc
     1321 agaacgtcgt cgccatgctg tcgctggtgc cctggggcaa caacggggtt tcgctcgact
     1381 tgatgcgacg ttcaccgcac tcgccgaacg gcaccatcga attgatggtg accgaactgc
     1441 tgcagcagtc cgaaggtatt gggatcaccc gtgtttcgct caacttcgcg atgttccggt
     1501 cggcgttcga gcaaggggcg cagctcggcg cgggccccgt tgcccggctg tggcgcgcac
     1561 tgctggtgtt cttctcacgg tggtggcagc tggagacgct gtatcgctcg aacatgaagt
     1621 acaagcccca atgggtaccg cggtacgcct gctacgaaga cgccaggctc gtcccgcgcg
     1681 tcggcgtcgc atcggtgatc gccgaaggct ttctggtgct tccgttctcg cgacgcaa
//
LOCUS       DASYLX010000382         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2321483,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000382 DASYLX010000000
VERSION     DASYLX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321483"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGP27_09410"
     CDS             complement(<1..296)
                     /locus_tag="VGP27_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV7851565.1"
                     /translation="MIFDHDGAEVGRHQLEHEQILPQAGWVEHNPVEIWERTASVLTS
                     VLNKTKLSATDLAALGITNQRETSLVWNKNTGRPYYNAIVWQDTRTDRIASALDR"
     gene            388..1161
                     /locus_tag="VGP27_09415"
     CDS             388..1161
                     /locus_tag="VGP27_09415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851566.1"
                     /translation="MDYMAREIVDIDRMPRGGPDASCLDRRLQTDRLEYLDRHDADEE
                     KKRSVIRALEWTGAVFGNTEKFARIALDEVADVPDPKILELGAGHGALSRTLLEWHPT
                     AELTVTDVETAWVQAIADGDLGAHHRATVREMDATAIDASDRHFDLAVFVLSFHHLSP
                     ALASQVLAEGTRAATKLLIIDLPRPPSALHIMRLATMLPFAPWLPFVHDGVISSLRTY
                     SPSALRALARHADPSIDIELRSGLMSPQIAVASRRSQSI"
     gene            complement(1149..1490)
                     /locus_tag="VGP27_09420"
     CDS             complement(1149..1490)
                     /locus_tag="VGP27_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HEV7851567.1"
                     /translation="MTESEPTTPTQTPPAALPPGPPPHNAGWAVAAVLFFWPLSFSAF
                     THAFNVYPLWAAGNFAGAQYASDRVRRLGQLSLWIAGGLLLLFVIFYAIFVMVLISQG
                     GDWGHHRGYMD"
     gene            complement(1552..2352)
                     /locus_tag="VGP27_09425"
     CDS             complement(1552..2352)
                     /locus_tag="VGP27_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7851568.1"
                     /translation="MEVTVTFIGNATTLISGGGVTLLTDPNFLHRGQHAYLGYGLVSK
                     RLREPAIDIDQLPPLEAVVLSHMHGDHWDRRTTTELDHELPVLTTPHAAKRLSHRGFG
                     SALGLQTWQKHNIVKGETVVNVTALPGRHAQAPIHRMLPPVMGSMLEFSSGGQTRRLY
                     VSGDTLLVEELSEIPVRFEAIDVGVLHLGGTRLPAGRRLPFGLTVTMDGRQGADAVEA
                     LKLPRMIPVHFDDYGVFASPLSDFVEEMKRRGIADRIIEIERGASVSI"
     gene            2432..>3573
                     /locus_tag="VGP27_09430"
     CDS             2432..>3573
                     /locus_tag="VGP27_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV7851569.1"
                     /translation="MGEEVRQTDFSRAHRREYRRKVQLCLDVFETMLTRSSFEFERPL
                     TGMEIECNLVDADYQPAMSNEEVLASIADPAYQTELGAYNIEFNVPPRPLPGRAALEL
                     ESDVRASLNAAESKANSSGSHIVMIGILPTLMPEHLSGDWMSSSTRYQALNDSIFVAR
                     GEDIQIDITGPERLSLQAASIAPESACTSMQLHLQVSPEDFAKNWNAAQVLAGPQLAL
                     GANSPYFFGHQLWAETRIELFAQATDTRPDELKAQGVRPRVWFGERWITSIFDLFEEN
                     VRYFPSLLPELSDEDPVAELAEGRTPKLAELRLHNGTIYRWNRPVYDVVDGRPHLRVE
                     NRVLPAGPTVIDMMANSAFYYGVLRSLSDDDRPLWTKMSFAAAHQN"
BASE COUNT          606 a         1193 c         1164 g          610 t
ORIGIN      
        1 cggtcgagcg ccgatgcgat gcggtcggtg cgggtgtcct gccagacgat cgcgttgtag
       61 tacgggcggc cggtgttctt gttccacacc agcgacgtct cccgctggtt ggtgataccc
      121 agcgccgcaa ggtcggtggc cgacagcttc gtcttgttca gcaccgacgt cagcaccgac
      181 gcggtgcgct cccatatctc caccgggttg tgctccaccc agcccgcctg cggcaggatc
      241 tgttcgtgtt cgagctgatg gcggccgact tcggcgccgt cgtggtcgaa gatcatggcg
      301 cgggtgctgg tggtgccctg gtcgatcgcc gcgatgaact ctgccaacta cctgctcccc
      361 ttctgacggc tccagtcgtc catcatcatg gactacatgg ctcgcgagat cgtcgacatt
      421 gatcgcatgc cccggggcgg gcccgacgca tcatgcctcg atcggcgact gcagaccgat
      481 cgcctggagt acctcgatcg ccacgacgcc gacgaggaga agaagcgcag cgtgatccgg
      541 gcgctcgagt ggaccggtgc ggtgttcggc aacaccgaga agttcgcgcg gatcgcgctc
      601 gacgaagtgg ccgacgtgcc cgaccccaag atcctcgaac tcggcgcagg ccacggtgcc
      661 ctgtcgcgca cgctgctcga gtggcatccg accgccgagc tgaccgtcac cgacgtggag
      721 accgcatggg tgcaggcgat cgccgacggt gacctcggcg cacaccaccg cgcgaccgtg
      781 cgtgagatgg acgcgacggc gatcgacgcg tcggatcggc acttcgatct ggccgtgttc
      841 gtgctgtcct tccaccacct gtcaccggca ctggcgtcgc aggtgctggc cgaaggcaca
      901 agggcggcca ccaagctgtt gatcatcgac ttgccgcggc cgccgtctgc cctgcatatc
      961 atgcggctcg cgaccatgct gcccttcgcg ccgtggctgc cgttcgtgca cgacggcgtg
     1021 atcagctctc tgcgtaccta cagcccgtcg gccctgcgtg cgctcgcccg acacgccgac
     1081 ccgtcgatcg acatcgagct gcgcagcggg ttgatgagcc cgcagatcgc ggtcgccagc
     1141 cgcagatctc agtccatata gccgcggtgg tggccccagt cgccgccctg ggagatcagc
     1201 accatcacga agatggcgta gaagatcacg aacagcagca gcaacccgcc cgcgatccac
     1261 agcgagagct gccccagccg gcgcacgcgg tccgacgcgt actgcgcgcc ggcgaagttt
     1321 cccgccgccc acagcggata gacgttgaac gcgtgggtga aagccgagaa cgacagtggc
     1381 cagaagaaca gcacggccgc gacggcccag ccggcgttgt gcggcggcgg gccgggcggt
     1441 aaagccgcag gcggggtctg cgtcggagtg gtcggttcgg attcagtcat gtttcggact
     1501 cctgtcgatg aactacgaca tctccgaacg tactgccatc gacggccggc gtcagatgct
     1561 gaccgaggct ccccgctcga tttcgatgat gcggtcggcg atgccgcgcc gcttcatctc
     1621 ctcgacgaag tccgacagcg gcgaggcgaa gacgccgtag tcgtcgaagt gcaccgggat
     1681 catcctgggc agtttcagcg cctcgaccgc gtcagcgccc tgcctgccgt ccatggtgac
     1741 cgtcaggccg aacggcaacc ggcgccccgc gggcagccgg gtgccgccga gatgcagcac
     1801 gccgacgtcg atggcctcga accgcaccgg gatctcactg agctcctcga ccagcagcgt
     1861 gtcaccggag acatagaggc ggcgcgtctg cccgccggag ctgaactcga gcatgctgcc
     1921 catcaccggc ggcagcatgc gatgtatcgg agcctgggcg tgcctgccgg gtagcgcggt
     1981 cacgttcacc acggtctcgc ccttgacgat gttgtgtttc tgccaggtct gcaggcccaa
     2041 cgcggagccg aatccgcggt gactaagccg ctttgcggcg tgcggcgtgg tgagtacggg
     2101 cagctcgtga tccagttcgg tggtcgtcct gcggtcccaa tggtcgccgt gcatgtgcga
     2161 cagcaccacc gcttccagcg gcggcaactg atcgatgtcg atagcgggtt cccgcaggcg
     2221 cttggacacc agcccgtagc cgagataggc gtgttgaccg cggtgcagaa agttcgggtc
     2281 ggtcagcagg gtgacgccgc cgccggagat cagcgtggtg gcgttgccga tgaaggtgac
     2341 ggtgacttcc atcgcccagg gctacccggc gcaccgcggt ttatgcacga tttccttgcg
     2401 cgcgcgccat ggagcctgtt ccatcggcgt tgtgggcgaa gaggtgaggc agaccgactt
     2461 cagtcgcgcg caccgccgcg aataccgacg caaggtccag ctgtgcctcg acgtcttcga
     2521 aacgatgctg acccggtcga gtttcgagtt tgagcgtccg ttgacgggca tggagatcga
     2581 gtgcaacctc gtcgacgccg actatcagcc cgcgatgagc aacgaggaag tgctcgcgtc
     2641 catcgccgat ccggcctacc agaccgaatt aggcgcctac aacatagaat tcaacgttcc
     2701 gccccgcccg ttgcccggcc gcgcggcact cgagctggag tccgatgtcc gggccagcct
     2761 caacgccgcg gaatccaagg cgaactcgag cggctcccac atcgtgatga tcggcattct
     2821 tccgacgctg atgcccgaac acctttcggg cgactggatg agctcgtcca cccgctacca
     2881 ggccctcaac gactccattt tcgtcgccag aggcgaggac atccagatcg acatcacggg
     2941 gccggagcgg ctgagcctgc aggccgcctc catcgcaccc gaatccgctt gcaccagtat
     3001 gcaattgcat ctgcaggtgt cgcccgaaga ctttgcgaag aactggaacg cggcgcaggt
     3061 gctggcaggc ccgcagctcg cgctgggcgc caactcgccc tatttcttcg ggcaccaact
     3121 gtgggcggag actcggatcg aactgttcgc ccaagccacc gacacccggc ccgacgagct
     3181 caaggcgcag ggcgtgcggc cgcgggtctg gttcggtgag cggtggatca cgtcgatctt
     3241 cgacctgttc gaggagaacg tccgctactt cccgtcgctg ttgcccgagc tgtccgacga
     3301 ggacccggtc gccgaactgg ccgagggccg caccccgaag ctcgcggagc tgcggttgca
     3361 caacggcacg atctaccggt ggaacaggcc ggtgtacgac gtcgtcgacg gccgacctca
     3421 ccttcgggtg gagaaccgcg tgctgcccgc aggcccgacc gtcatcgaca tgatggccaa
     3481 ctcggcgttc tactacgggg tgttgcggag cctgtcggac gacgatcgtc cgctgtggac
     3541 gaagatgagt ttcgctgcgg cacatcagaa ttt
//
LOCUS       DASYLX010000383         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1114022,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000383 DASYLX010000000
VERSION     DASYLX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114022"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGP27_09435"
     CDS             <1..236
                     /locus_tag="VGP27_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7851570.1"
                     /translation="EMRHGKELTWSQVVMAANTPMLLKAGLVEGNTDAGVLASGQVAG
                     ILGDLPSCAELIESIVGDAVKHLQAAVAFVNEG"
     gene            273..926
                     /locus_tag="VGP27_09440"
     CDS             273..926
                     /locus_tag="VGP27_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEV7851571.1"
                     /translation="MSRVKTAASSGVKATLIGDVVGSRSLADRAGVHRLLNRALRDLT
                     DVAIDPPAFTVGDEFQGAFTSVGLAIDAALSIRLAVAPGIDVRFGLGWGAVTMLDETA
                     GIQDGPGWWAAREAIEWTASVQRQPGLALVRTSFRAAEGTRTDLDAINAALICRDHLL
                     GSLDDRSTRIVKAMLDNRTKKDIAAAEGISPSAVSQRSGRDGLDLIVVASEYLRSVP"
     gene            923..1657
                     /locus_tag="VGP27_09445"
     CDS             923..1657
                     /locus_tag="VGP27_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851572.1"
                     /translation="MSALAVMLIAMGIADAVRRLVRRVWVPAVAAPTVVVVCAALSGL
                     WHHGDIPLLIIAAAAGASWVLLGARAERGAHEGAPLAVFGLALAALILLSGWASDVGG
                     LVARWSSWAEVPGIGDVRPNRLLMVVGAVLLQFVTGNQLVRLVLGSVGAVKPEGQPQP
                     SDRLKGGRLLGPMERVLIFGLGLAGQLAAATAVVAAKSIIRFPEINAAKARENGNIGI
                     DAVTEYFLVGSFASWIIALGGLALAQ"
     gene            complement(1662..2810)
                     /locus_tag="VGP27_09450"
     CDS             complement(1662..2810)
                     /locus_tag="VGP27_09450"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7851573.1"
                     /translation="MPEAYVVEAVRTAVGKRNGSLAGVHPVDLGAAAWRGLFDRVDAD
                     PGAVDDVIAGCVDAIGPQAGNIARLSWLAAGYPEEVPGVTVDRQCGSSQQAISFGAQA
                     IMSGTADLIVAGGVQNMSQIPISSAMTVAEQFGFTSPTNESKSWLQRYGDQEISQFRG
                     AELIAEKWNISREEMEEYSLTSHQRAQEAIRAGYFENEIIPVDGFVTDEGPRDTSLEK
                     MAGLKTLVDGGRLTAAMASQISDGASAVLLASEQAVKDHGLKPRARIHHISARGADPV
                     FMLTGPIPATRYALEKSGLTIDDIDTVEINEAFAPVVMAWLKETKADPKKVNPNGGAI
                     ALGHPLGATGAKLFTTMLNELERTGGRYGLQTMCEGGGTANVTIIERL"
     gene            complement(2812..3417)
                     /gene="kstR2"
                     /locus_tag="VGP27_09455"
     CDS             complement(2812..3417)
                     /gene="kstR2"
                     /locus_tag="VGP27_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator KstR2"
                     /protein_id="HEV7851574.1"
                     /translation="MTAQPGPQSASRRDELLQLAATMFAERGLRATTVRDIADSAGIL
                     SGSLYHHFKSKEQMVEEVLRDFLDWLFGRYREIVDTEPDPLARLKGLFMASFEAIEHR
                     HAQVVIYQDEAKRLSALPQFDFVEARNKEQRKMWVDVLNQGVGEGCLRPDLDVDLVYR
                     FIRDTTWVSVRWYQPGGPLTAEQVGRHYLAIVLGGITKEGA"
     gene            complement(3414..>3578)
                     /locus_tag="VGP27_09460"
     CDS             complement(3414..>3578)
                     /locus_tag="VGP27_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851575.1"
                     /translation="EKTSSADLLDRLSEGEAFGRAAEPWEVAATIAFLASDYSSYLTG
                     EVISVSSQRA"
BASE COUNT          587 a         1168 c         1151 g          672 t
ORIGIN      
        1 tcgagatgcg ccacggcaag gaactcacct ggtctcaggt cgtgatggcg gccaacacac
       61 cgatgctgct gaaggccggc ctcgtcgaag gcaacaccga tgcgggtgta ctggcgtccg
      121 ggcaggttgc cggcatcctc ggcgacctgc cgtcatgcgc cgaactgatc gaatcgatcg
      181 tcggcgacgc agtcaaacac ctgcaggcgg ctgtcgcatt cgtaaatgaa ggttaaagct
      241 taattttcaa tagctgaagc tatactcttc atatgtctag ggtgaagacc gcagcttcat
      301 caggtgttaa ggcgaccctc atcggcgacg tcgtcggatc acgatcgctc gccgaccgcg
      361 cgggcgtaca ccgactgctc aaccgtgcgc ttcgcgatct caccgacgtg gcgatcgacc
      421 cgcccgcctt cacagtcggc gacgagttcc aaggcgcgtt caccagtgtc ggtctggcga
      481 tcgacgcagc gctgtcaatc cggttggccg tcgcacccgg aatcgacgtg cggttcggcc
      541 tcggctgggg cgcggtcacc atgctcgacg agacagcggg catccaggac ggccccggct
      601 ggtgggccgc ccgcgaagcc atcgagtgga cggcatcggt tcaacgccag cccggacttg
      661 cgctggtacg gacgtccttc cgggccgctg agggaaccag gaccgacctc gacgcgatca
      721 acgcggcact gatttgtcgg gaccacctgc ttggatcgtt ggacgaccga tcgacacgga
      781 ttgtgaaggc aatgttggac aaccggacca aaaaggacat cgccgcggct gaaggcatca
      841 gcccgtcggc agtgtcgcag cgctccggcc gcgacggtct cgatttgatt gtcgttgctt
      901 cggagtatct gcggagcgtg ccgtgagcgc gctggcggta atgctcatcg caatgggcat
      961 cgccgatgcg gtccggcgcc tggtccgccg ggtttgggtg cctgcggtgg ccgcgcccac
     1021 cgtggtggtg gtctgcgcgg cactgtccgg gctgtggcat cacggcgaca tcccgctgtt
     1081 gatcatcgcg gccgccgccg gcgcgagctg ggtcctgctg ggtgcccgcg cggaacgcgg
     1141 cgcccacgag ggcgcaccgc tggcggtgtt cgggctcgcg ctcgccgccc tgatcctgtt
     1201 gtcggggtgg gcttccgacg tcggtggtct cgtcgcgcga tggtcgtcgt gggccgaggt
     1261 gccaggtatc ggcgatgtgc gcccgaaccg gctgctgatg gtcgtcggcg cagtactgct
     1321 gcagttcgtc accggcaacc agttggtgcg gctggtgctc ggttcggtcg gcgctgtgaa
     1381 acccgagggt cagccgcagc cttcggaccg gctcaagggc ggtcgcctgc ttgggccgat
     1441 ggaaagagtc ctgatcttcg ggctcggcct ggccggtcag ctcgcagcgg ccaccgccgt
     1501 cgtcgccgcg aagagcatca tccgttttcc ggagatcaac gctgccaagg cccgcgagaa
     1561 cgggaacatc ggcatcgacg ccgtcaccga atactttctc gtcggcagtt tcgccagctg
     1621 gatcatcgcg ctcggcgggc ttgcgctcgc gcagtagcgc gctagagccg ctcgatgatg
     1681 gtgacgttgg cggttccgcc gccctcgcac atcgtctgca gcccgtagcg gccgccagtg
     1741 cgctccagct cgttgagcat ggtggtgaac agctttgcgc cggtagcacc caacgggtga
     1801 ccgagcgcga tcgcaccgcc gttcggattc acctttttcg ggtcggcctt ggtttccttg
     1861 agccaggcca tgacaaccgg cgcgaaggcc tcgttgatct ccacggtgtc gatgtcgtcg
     1921 atcgtcaacc cgctcttctc gagcgcatac cgggttgcgg gaatcgggcc cgtcagcatg
     1981 aaaaccgggt cggcgccgcg ggcgctgatg tgatggatac gggcgcgcgg cttgaggccg
     2041 tgatccttga cggcctgctc cgacgccagc agcacggcgc tggcgccgtc cgagatctga
     2101 ctggccatcg cggcagtcaa ccgaccaccg tcgaccaggg tcttgaggcc ggccatcttc
     2161 tccaaagaag tgtcgcgcgg gccttcgtcg gtgacgaacc catcgaccgg gatgatctcg
     2221 ttctcgaagt atccggcgcg gatcgcttcc tgcgcacgct gatggctggt gagcgagtac
     2281 tcctccatct cctcacgcga gatgttccac ttctcggcga tcagctccgc cccgcggaac
     2341 tgtgagatct cctgatcgcc gtagcgctgc agccagctct tcgactcgtt ggtgggcgag
     2401 gtgaagccga actgctcggc gaccgtcatt gccgacgaga tcgggatctg gctcatgttc
     2461 tgaacaccgc cggcgacgat cagatcagcg gtgccggaca tgatcgcttg agcgccaaag
     2521 gaaatagcct gttgacttga accgcactgg cgatccaccg tgacacccgg cacttcttcc
     2581 gggtatccgg cggcaagcca ggacaatctc gcgatgttcc ctgcctgggg cccgatggca
     2641 tcgacacatc cggcgatcac gtcgtccacg gcgcccggat cggcgtcgac ccgatcgaac
     2701 agtccgcgcc acgctgcggc ccccagatcc acgggatgca cacccgccag tgatccgttt
     2761 cgcttgccga ccgcggtgcg tacggcttcg actacatatg cctcaggcat ttcaagctcc
     2821 ttctttggtg atgccgccaa ggactatggc caggtaatgc cggccgacct gctcggccgt
     2881 caacggtccg cctggctgat accagcgaac tgaaacccac gtggtgtcgc ggatgaaccg
     2941 gtagaccaga tcgacgtcga gatccgggcg caggcagccc tccccgaccc cctggttcag
     3001 gacgtccacc cacatcttgc gttgctcctt gttgcgagct tcgacgaagt cgaactgcgg
     3061 cagcgccgaa agccgtttgg cctcgtcctg gtaaatgacg acctgggcgt gccggtgctc
     3121 gatggcctcg aaggatgcca tgaacaatcc cttgagccgc gcgagcggat ccggctcggt
     3181 gtcaacgatt tcccgatacc gaccgaacag ccagtcgagg aaatcgcgca gcacctcctc
     3241 gaccatctgc tccttgctct tgaaatggtg gtagaggctt ccagacagta ttcccgcgga
     3301 gtccgcgatg tctcgcaccg tcgtcgcgcg caggccgcgc tcggcgaaca tcgtcgcggc
     3361 gagttggaga agctcatcgc gtctgctcgc cgactgcggg cccggctgcg cagtcatgct
     3421 cgctggctcg acaccgaaat gacttctccg gtcaggtaac tggagtagtc actggccagg
     3481 aacgcgatgg tggccgcgac ctcccatggt tcggccgccc ggccgaaagc ctcgccctcg
     3541 gacagtcggt cgagcagatc ggccgaactc gtcttctc
//
LOCUS       DASYLX010000384         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2210885,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000384 DASYLX010000000
VERSION     DASYLX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210885"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGP27_09465"
     CDS             <1..236
                     /locus_tag="VGP27_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851576.1"
                     /translation="MPVGLRNRIDVYFDPDHAGWFTSAQAAKDVTLFLELAKGGAPLP
                     VTAAARDAYRQVARDGWVTNDVTALVEYGRRSP"
     gene            612..1277
                     /locus_tag="VGP27_09470"
                     /pseudo
     CDS             612..1277
                     /locus_tag="VGP27_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
     gene            1448..>2234
                     /locus_tag="VGP27_09475"
     CDS             1448..>2234
                     /locus_tag="VGP27_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7851577.1"
                     /translation="MTTQPRHSHRKQGRRSPDQQPHKRSRGVALFAAALAIAVVSAGI
                     GAVAAPDLYSQLATRSTVATVSAAASRPTSDSNEQVAAKVVPSVVELQTDLGNHSDVG
                     SGVILSPGGLIMTNAHVVSAAQQPAPAAPGGVHTIVTFADGRTAPFTVVATDPTCDIA
                     VVRADGTSGLTPITFGSSTDLRVGQQVVAVGSPLGLDGTVTAGIISALDRPVSTAAGS
                     TNQATAIDAIQTDAPLNPGNSGGALVNANGQLIGMNSANASLGA"
BASE COUNT          400 a          756 c          707 g          371 t
ORIGIN      
        1 tgatgcccgt tggtttacgc aaccgcatcg acgtctactt cgaccccgac cacgccggtt
       61 ggttcaccag cgcccaggcc gccaaggatg tcacgctctt cttggagctc gccaaaggtg
      121 gcgcgccact gccggtcacg gcggccgcgc gcgacgccta ccgtcaggtt gcccgggatg
      181 gatgggtgac gaacgacgtc accgccctcg tcgagtacgg ccgccgttcg ccgtgactga
      241 gacggtcgcg gggacggccc gcaactcgtc gggtcatatc cgcgtgcgtt cgcaccgaaa
      301 cacgttacgt cccttcgcaa gtcgatctgc gaccgcgacg cccaagtcaa gccgaagctg
      361 agaccttccg gcgctgagta acccctacca gcggcaagta ggcggcccag ataccggctg
      421 gcataccgcc ttgatgggcg ccagcggtag cgacattcgc ttgctcgacc gggatcgtgg
      481 caggtgcatc gtccaccctt ggcactcgac gagacgatgc tgatccgccc attgatcgga
      541 gaagccagat gccccacgcc cctgtagcga tcgataccgc cttgatgccc gcaccgcgcc
      601 ggcgggaaga gatcgacgag gtgcttgcca ggagcgggat attcggcaga gtcgaggccg
      661 gcgcgcgctc cgcgctgacc gaacggcttc agcccgttga cttcccagcc gggcacaccg
      721 tctacaccga aggcgacccc ggtgaccgca tgtacatcat catttccggg aaggtcaaga
      781 tgggccgcct ttcccgcgat ggcggcgagg atctgctcgc agtcataggc ccttcggaga
      841 tgttcggcga gatgtcgacg ttcgacccgg gtccgcgtac ctccagagca accaccatca
      901 gcgaagtccg cgccgtgtcg attggccggg acacgctgcg ccccttgatg tttgaccacc
      961 ccacgatagc cgagcagctg cttcgggtgc taggtcgcag actacggcgt acgaccaacc
     1021 tggccgatct gatctacacc gacgttcccg gccgagtggc caagcgactg ctccacttgg
     1081 cgcatcggtt cggcgtccag gaggacggcg cgttgcgagt cacccatgac ttgacggacg
     1141 aggagatggg ccagctggtc ggcgcatcac cggacacggt taacgacgcg ttggccgatt
     1201 ttgccctccg cgggtggatt cggcttgatg acaccggcgt gttgatctcc gaatccgaac
     1261 tactgggccg gcgggcgagc gcgggtgtcg atcgcagcga tcggggtgac tcattgcagc
     1321 tggcaggtat cgctaatttg ccgctggcag gaggtggcga tggacgccag cgctagcgac
     1381 accaggcacc gacaaacgcg attctagaag catgttcacg cttcctacag cgaaggaccg
     1441 cagcgacatg accactcagc cgaggcattc gcatcgcaaa caaggtcgaa gaagcccaga
     1501 tcagcagccg cacaagcgtt cccgaggcgt ggcactcttc gccgctgcgc tggcgatcgc
     1561 ggtggtgtcc gctggtatcg gcgcagtggc cgcgccagac ctgtattcgc aactggccac
     1621 caggagcacc gtggcgacgg tctcggcggc cgcgtcccgg ccgaccagcg actcgaacga
     1681 gcaggttgcc gcaaaggtgg tgcccagcgt cgtcgaactg cagaccgacc tgggcaatca
     1741 cagtgacgtc ggctcaggcg tcatcctgtc ccccggtggg ctgatcatga ccaacgcaca
     1801 cgtcgtgtcg gccgcacagc agcccgcccc ggccgccccg ggcggcgtgc acaccatcgt
     1861 gacgttcgcc gatggccgca ccgccccctt caccgtcgtc gcgaccgatc cgacatgtga
     1921 tatcgccgtt gtgagggccg acggaacgtc tggactcacc ccgattacct tcggctcctc
     1981 aaccgaccta cgcgtcggcc agcaggtggt ggcggtgggt tcgccactgg gtttggacgg
     2041 cacagtcaca gcgggcatca tcagcgcttt ggaccggccg gtgtctaccg ctgccggttc
     2101 aacgaatcag gccacggcga tcgacgccat ccagaccgac gccccgctca accccggcaa
     2161 ctcgggcggg gccttggtga atgcgaacgg ccagctcatc ggaatgaact cggcgaacgc
     2221 gtcgcttggc gccc
//
LOCUS       DASYLX010000385         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2759019,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000385 DASYLX010000000
VERSION     DASYLX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759019"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGP27_09480"
     CDS             <1..184
                     /locus_tag="VGP27_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7851578.1"
                     /translation="TVTARYDQAAVTDGDYFARCLQAGFDEVLTLGGEGRCVPASFTT
                     DADERRPPSPNGSSAL"
     gene            181..1860
                     /locus_tag="VGP27_09485"
     CDS             181..1860
                     /locus_tag="VGP27_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase/lysophospholipid
                     acyltransferase family protein"
                     /protein_id="HEV7851579.1"
                     /translation="MTAPDGEPTRRANPRTMRLPGSVAEIKGSPAGPKIGAFFDLDGT
                     LVAGFTGVIMTRDRLRRGQMGVGEFIGMVQAGLNHQLGRSEFEDLIGKGARMLRGSSL
                     GDIDELAERLFMQKIIGRIYPEMRDLVRAHMDRGHTVVLSSSALTVQVEPVARFLGIK
                     NVLSNKFETDDNGLITGEVKTPIIWGPGKARAVQAFAAEHGVDLAKSYFYADGDEDVA
                     LMYLVGNPRPTNPAGKLAAVAAKRGWPVLRFSSRSGSNPVSQLRTAAGVASLVPIAAG
                     AIGLGLLTRNRRTGVNFFTSAFGRTLLTATGVNINLLGKENLTKKRPAVFIFNHRNQV
                     DPIIAGRLVDTNFTSVGKKELENDPIAGTLGKVMDAAFIDRDNPKAAIEGLKKVEENA
                     RKGLSILIAPEGTRLDTTGVGPFKKGPFRIAMSAGIPIVPIVIRNAEVISARDSTTFN
                     PGTVDVVVYPPIPVDDWTVENLSDKIAEVRQLYLDTLNDWPHDSVPKIGPYARNRSAK
                     KAPAKKSKKAAPPKKSSPRKAPEKQTAEATVKKAAAKKAPAKKAAVKKGRS"
     gene            1872..4196
                     /locus_tag="VGP27_09490"
     CDS             1872..4196
                     /locus_tag="VGP27_09490"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HEV7851580.1"
                     /translation="MAPFSATDDALVLASVSSAAERELLSDWLQQQRREHPESNVAVL
                     ELPDGDPPPGVLARLVGELEGDEDRSVVPVRVFWIPGGLPTRSKVVSLISGRDTYRPP
                     EILQRRILKRDPSRARVVAGEPAKVSELRQQWSDTTVGENPRDLARFVIRRASLAIER
                     VELRLLGPEYKSPQLIKPEILESARFRDGLEKIPGATVEAAGKMLDELSTGWSRFSVD
                     LIPSLGRAIFSRGFDPKIDYDRAEIENMRHALESHPAVLLFSHRSYLDGVIVPVAMQE
                     NRLPPVHTFAGINLSFGFMGPLMRRSGVIFIRRKLDDPLYKYVLRQFVGYIVQKRFNL
                     SWSIEGTRSRTGKMLPPKLGLMSYVADAYLEGRSEDILLQPVSISFDQLHETAEYAAY
                     ARGGEKTPEGLSWLYKYIKAQGERSYGKIYVRFPQAVSMREYLGAPGGPIAEDEAAKR
                     LAMQKMAIEVSWRILRATPVNATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQ
                     TPVTNSVLRLRTADGVRAALDALSNGHPVTCVDAGREPVWRIAPEQEHEAAFYRNTII
                     DAFMETAIVELALAYAARADANRLEAFWTQAMRLRDLLKFDFYFAESTAFREHIAEEM
                     AWNDDWEAHIEAGGDEIGAMLHAKRPLISQAMLRPYIEAYEIVADVLGDLPAEVDEKE
                     LTKRALGVGNQYIAQNRVRTTESVSALLFATARQVVADQHLLEPAADLLERRTAFRDE
                     LRDVLRDMDKVEEMAREQFYDREMERRTLREAAE"
     gene            4227..>5414
                     /locus_tag="VGP27_09495"
     CDS             4227..>5414
                     /locus_tag="VGP27_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEV7851581.1"
                     /translation="MTTAPSVRTSAVWPLLFGVALLAGVTAAGLGAMTLADALTATGL
                     PDPGPVTTYGLPFMRAAGEIAAVVAVGSFMFAAFMVPPQPNGVLDADGYRALRLGTVA
                     SAIWTVCAALLVPLTVSDVTGQPLVSRLSLSDIWSVAGLVETAGAWRWTALLAAIVTV
                     ASIPVLRWSPAPLLFAGSLVTLLPLALTGHSSTGGSHDLATNSLLIHLIAAALWAGGL
                     LALLVHTLRRGGHADLAARRFSAVAIWCFVAMALSGVVNALVRIRPSELFDGGYGLLI
                     LAKAGALCTLGVIGWRQRRNGVAALQADPEARGALIRLAITEAVVFGLTFGIAVALSR
                     TPPPSPPIFNPSIPAIEIGYDFAGPPTVARVLFDWRFDLIFGTAAIVMALVYLAGVRR
                     LRRR"
BASE COUNT          944 a         1776 c         1800 g          894 t
ORIGIN      
        1 cacggtcacc gcacggtatg accaggccgc ggtcaccgat ggcgactact tcgccaggtg
       61 cctgcaagcc ggattcgacg aagtgctcac gctcggcggc gaaggacgtt gcgtgccagc
      121 atcattcaca acggacgccg atgaacgccg tcccccgtcg ccgaacggaa gtagcgccct
      181 atgacagccc cagacggcga gccgacacga cgcgccaacc cgcgaaccat gcgcctgccc
      241 ggttcggtag ccgaaatcaa gggcagccct gccggtccga agatcggtgc cttcttcgat
      301 ctcgacggca cacttgtcgc cggcttcacc ggcgtgatca tgacccgaga tcggttgcga
      361 cgcggtcaga tgggcgtcgg cgagttcatc ggcatggtgc aggccggcct caaccatcag
      421 ctcggtcgct ctgagttcga agacctgatc ggcaagggcg cgcgcatgtt gcgggggagt
      481 tcgctcggtg acatcgacga gctcgccgag cggctcttca tgcagaagat catcggccgg
      541 atctaccccg agatgcggga tctggtgcgc gcccacatgg atcgcggcca caccgtcgtg
      601 ctcagctcgt cggcgctcac ggttcaggtg gaaccggtgg cgcgcttcct cggcatcaag
      661 aacgtgctca gcaacaaatt cgagacggac gacaatgggc tgatcaccgg cgaagtgaag
      721 accccgatca tctggggtcc gggcaaggcc agggcagtgc aggcgttcgc cgccgaacac
      781 ggcgtcgacc ttgcgaaaag ctacttctac gccgacggtg acgaagatgt cgccctgatg
      841 tatctggtgg gcaatccgcg gccaaccaac ccggcaggaa agctcgccgc cgttgcggcc
      901 aaacgaggtt ggccggtgct gcggttcagc agccgcagcg gcagcaaccc ggtgtcgcaa
      961 ctgcgtaccg ccgcaggcgt cgcatcgctg gtgccgatcg cggcaggcgc gatcgggctc
     1021 ggcctactga cgcgcaacag gcgcaccggc gtcaacttct tcacatcggc cttcgggcga
     1081 acgctgctga cggccaccgg cgtgaacatc aacctgctcg gcaaggagaa cctgacgaag
     1141 aagcggcctg cggtgttcat cttcaaccac cgcaaccagg tcgatcccat catcgccggt
     1201 cggctcgtcg acaccaactt cacctcggtc ggcaagaagg agctggagaa cgacccgatc
     1261 gccggcaccc tgggcaaggt gatggacgcg gcgttcatcg atcgggacaa cccgaaggcc
     1321 gcgatcgagg gcttgaagaa ggtcgaggag aacgccagga aggggctctc gatcctcatc
     1381 gcgccggagg gcacccgcct cgacaccact ggagtgggtc cgttcaagaa gggccccttc
     1441 cgtatcgcga tgtccgcggg aatacccatc gtgcccatcg tcatccgcaa tgcagaggtg
     1501 atttcggcgc gcgattcgac gaccttcaat cccggcaccg tcgacgtcgt ggtgtatccg
     1561 cctatcccgg tggatgattg gacggtcgag aacctgtcgg acaagatcgc cgaagtgcgt
     1621 cagctctatc tcgacacgtt gaacgactgg ccgcacgact cggttccgaa gatcggacca
     1681 tacgcgcgga acagaagtgc caagaaggcg ccggccaaga aatccaaaaa ggccgcgcca
     1741 ccgaagaagt cgtcaccgag gaaggcgccc gagaagcaga cggccgaggc gacagtgaag
     1801 aaggcggccg ccaagaaggc tcccgccaaa aaagctgccg tcaagaaagg ccggtcgtga
     1861 ccgccgatcc gatggcgccc ttctccgcca ccgacgacgc gctggtgctg gcgtccgtgt
     1921 cctcggccgc tgaacgagag ttgttgagcg actggctgca acagcagcga cgtgagcatc
     1981 ccgaatccaa cgtcgccgtg ctcgagcttc cagacgggga cccgccgccg ggtgtgctgg
     2041 cgaggctggt tggggaactc gaaggcgacg aagaccgatc cgtcgtcccg gtgcgcgtct
     2101 tctggatacc cggcggcctg ccgacgcgtt cgaaggtggt ctcattgatc tccggccgcg
     2161 atacctaccg gccgccggag atcctgcaac gccgcatcct caagcgggac ccgtcgcggg
     2221 ctcgcgtggt ggcgggagag ccggcgaagg tgtccgagct gcgccagcag tggagcgaca
     2281 ccaccgtcgg cgaaaacccc cgagatcttg cacgtttcgt catccgacgc gcaagcctcg
     2341 ccatcgaacg cgtcgagctt cggttactcg ggcccgagta caaatcgcct cagctgatca
     2401 agccggagat cctggagtcc gcgcggttcc gagacggcct cgagaagata cccggcgcga
     2461 ccgtggaggc agcgggaaag atgctcgacg aactgagcac cgggtggagc cggttctccg
     2521 tcgacctcat tccgtcgttg ggccgggcca tcttcagccg cggtttcgac ccgaagatcg
     2581 actatgaccg cgccgagatc gagaacatgc gacacgcgtt ggagagtcat cccgcggtgc
     2641 tgctgttctc ccaccgctcc tatctcgacg gcgtcatcgt gcccgtcgcc atgcaggaga
     2701 accggctgcc gccggtgcac actttcgcgg gcatcaacct gtcgttcggt ttcatggggc
     2761 cgttgatgcg tcgctccggc gtcatcttca tccggcgcaa acttgacgac ccgctgtaca
     2821 agtatgtgct gcgccagttc gtcggctaca tcgtccaaaa gcggttcaac ctgagctggt
     2881 cgatcgaggg cacccgatcg agaaccggaa agatgttgcc gcccaagctc ggtttgatga
     2941 gctacgtcgc cgacgcctac ctcgaggggc gcagcgagga catcctcctc cagccggtgt
     3001 cgatcagctt cgaccagttg cacgagaccg ccgaatacgc cgcgtacgcc cgtggcggag
     3061 agaagacgcc cgaggggctg tcgtggctgt acaagtacat caaagcccaa ggcgagcgaa
     3121 gctacggcaa gatctatgtc cgtttccctc aagccgtgtc gatgcgggag tacctcggcg
     3181 caccgggtgg accgatcgcc gaagacgaag ccgcgaagcg tcttgccatg cagaagatgg
     3241 cgatcgaggt ctcctggcga attctgcgcg ccacacccgt gaacgcgacc gggctggtgt
     3301 cagcgctgct gctgaccacc cgcggcaccg ctctgacgct ggaccagctg catcacacgt
     3361 tgcaggactc gctcgattac cttgagcgca aacagactcc ggtgaccaac agcgtgctgc
     3421 ggttacgcac cgccgacggc gttcgcgctg cgctggacgc gctgtccaac ggccatccgg
     3481 tcacctgcgt cgacgctgga cgggagccgg tctggcggat tgctcccgag caggagcacg
     3541 aagcggcgtt ctaccgcaac acgatcatcg acgcgttcat ggagaccgcg atcgtcgagc
     3601 tggcgttggc ctatgccgcg cgcgccgacg caaatcgcct ggaggcgttc tggactcagg
     3661 cgatgcggtt gcgcgacctg ttgaagttcg acttctactt cgcggagtcg acagccttcc
     3721 gtgagcacat cgccgaggag atggcgtgga acgacgactg ggaggcgcat atcgaggccg
     3781 gcggcgacga gatcggcgcg atgctgcacg ccaagcgacc attgatctcg caggcaatgc
     3841 tgcggccgta catcgaggcg tacgagatcg tcgccgacgt gttgggcgac ctgcccgccg
     3901 aggtcgacga gaaggaactg acgaagaggg cgctcggggt cggcaaccaa tacatcgcac
     3961 agaaccgcgt gcgcaccacc gagtcggtgt cggcgctgct gttcgccaca gcgcgtcaag
     4021 tggtcgccga tcagcacctg ctggagcctg cggcggatct cctcgaacgg cggaccgcgt
     4081 tccgcgacga gcttcgcgac gtgctgcgcg atatggacaa ggtcgaggag atggcccgcg
     4141 agcagttcta cgaccgtgag atggagcgac gcactttgcg cgaagcggcc gagtagctcg
     4201 ccggtcgtgg gccatacgct ggagccatga ccacggctcc aagcgtccgt accagcgcgg
     4261 tgtggcctct gctgttcggg gtcgccctgc tggccggggt gaccgcggcc ggcctgggcg
     4321 cgatgacgct ggccgacgcg ctgaccgcga cggggctgcc cgaccctggt ccggtgacca
     4381 cctacggcct gccgttcatg cgggcagcgg gcgagatcgc cgctgtcgtc gccgtgggtt
     4441 cgttcatgtt cgccgcgttc atggtgccgc cacagccgaa cggcgtgctg gacgccgacg
     4501 gataccgggc actgcgcctc ggcaccgtcg cgtcggcaat ctggacggtg tgtgccgcgt
     4561 tgctggtgcc gctgaccgtc tccgacgtca cgggtcagcc gctggtgtcg cggctcagcc
     4621 tgtcggacat ctggtcggtg gccggcctcg tcgagaccgc gggcgcgtgg cggtggacgg
     4681 cgctgctggc ggcgatcgtc acggtcgcga gcatcccggt gttgcgctgg tcgccggcgc
     4741 cgctgctgtt cgcgggctcg ctggtcactc tgctcccgct tgcgctcacc ggccattcgt
     4801 caacgggcgg ctcgcacgac ctggccacca acagcctgct catccaccta atcgccgccg
     4861 ccctgtgggc gggcggtctg ctggcgctcc tcgtccacac gctgcggcga ggcgggcacg
     4921 ccgatctcgc tgcgcgccgc ttctcggccg tcgcaatatg gtgcttcgtg gcgatggcgc
     4981 tcagtggcgt ggtgaacgcc ctggtgcgga ttcggcccag cgagttgttc gacggcggct
     5041 acggcctgtt gatcctcgcc aaggccggtg cgctgtgcac gctcggcgtc atcggctggc
     5101 ggcagcggcg caacggcgtc gctgcactgc aagccgaccc ggaggcgcgc ggcgcgctga
     5161 tcaggctggc gatcaccgag gcggtggtct tcggcctcac cttcgggata gcggtggcac
     5221 tgagccggac gcctccgccg tcgccgccga tcttcaatcc gtccattccg gcaatcgaga
     5281 tcggctacga cttcgccgga ccgccgacag tggcccgcgt gctgttcgac tggcggttcg
     5341 acctgatctt cgggaccgcg gcgatcgtga tggcgctggt gtacctggcc ggggtgcgac
     5401 ggttgcgccg gcgc
//
LOCUS       DASYLX010000386         7153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3197986,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000386 DASYLX010000000
VERSION     DASYLX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197986"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGP27_09500"
     CDS             complement(<1..237)
                     /locus_tag="VGP27_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7851582.1"
                     /translation="MYPFRFTKASDEASALTAAASGGRYIAGGTTLVDLMRETVERPE
                     AIVDINALPYRDIDFQPSRLRIGALARMSEVAAHP"
     gene            complement(241..810)
                     /locus_tag="VGP27_09505"
     CDS             complement(241..810)
                     /locus_tag="VGP27_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7851583.1"
                     /translation="MNRRTFVGGSIAVGGVVAASPLIAGCGEDEHSGDAQSSTVRLRI
                     NGEDREITVDNRTSLLDMLRERAGLTGTKKGCDQGACGACTILLDGRRINSCLTLAVM
                     HDGAEITTIEGLERNGRLHPLQQAFIDTDAFQCGYCTSGQIVSGVGCISEGHTGSSAE
                     IREWMSGNICRCGAYTNIVTAVAGAARAT"
     gene            complement(856..1734)
                     /locus_tag="VGP27_09510"
     CDS             complement(856..1734)
                     /locus_tag="VGP27_09510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851584.1"
                     /translation="MRGPLEWWNNPWRPPRFLVAITVGYLAWSLLPVLIAVAYSFNDG
                     RSRTSWQGFSFRWYWGDQTLSVWHNDALHTALLQTIKLGVIATLITVPLGTLFAIGID
                     RWRGRLPAGANFLMLLSFVLPEVLLAVALLFVITTVAVPVDLGTTAQVIGLVTFQVSY
                     PAVLVRARLATIGPQYEEAAMDLGASPLGALRRIIVPMLLPAIFASTVLVFADVIDDF
                     VLVRYLSGNAATEPVSVKIYNTARAAPTPALNALATLLLVAALAAVVIGFFIYRRMTR
                     GDDTADRGLGGFTGEA"
     gene            complement(1731..2672)
                     /locus_tag="VGP27_09515"
     CDS             complement(1731..2672)
                     /locus_tag="VGP27_09515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7851585.1"
                     /translation="MVAPVVAAPSRTASPPRERRSRLWSGLAAPGLIWLAAFFVFPLY
                     VVLCIVFGQVDPIFRTPVPVWNPLQWDPTQFTYVLTHIVGSNGVYGAAIVRTFTFVIV
                     ASSLCLLIAFPVAYYVARLSGKRKGLLLALLIAPFWISYMMRMFAWVNLLQDDGLLNR
                     VLGFGGFFTPDVKWLTGQPVVVILGLVYGYVPYMILPLYAGLDRLSQPMLEASRDLGA
                     DRISSFWRVTLPLCRPTIVAAVLLTCLPMLGDYFTSDMLSASPKTTMVGNLINDSVQS
                     PGQTGQAGAFVMLVFLAALLPMLYYIRVTSKPGEVST"
     gene            complement(2678..3919)
                     /locus_tag="VGP27_09520"
     CDS             complement(2678..3919)
                     /locus_tag="VGP27_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7851586.1"
                     /translation="MPVPSPSRRQFLTRAAILAAAAPSLGGFLAACSKSGPSSSQPTL
                     TIAAPDKPVKWDIRDDNKPIGDGLAPEQNATLQVYNYADYLSPDAVKSFEDQYKTKVQ
                     ISTFNDTDEALTKIRGGNVDYDLYFPSYDQISKLVTGGLARPLNHSYIPNIKNVWPTF
                     TNPWYDQEWRYSAPNFIYTTGIGWRNDQVPENIGALSNPYDALWDPKYKNKTAIIDDW
                     HTAMAMVLLRAGKTDVNTSSEADLKLVGDQLNALVAATSPKVTITMYSDMPAGQIGLA
                     QMWSGDIINGALSYMPEGQSPEVLRYWFPEDGKGLVDNDLFVMLRGGKNPVLAHLFIN
                     HLLDPEVAQQNFAQTGYQPPQNNINPDSLVSSGFIPPNLKSAVVKPEYFDAGYRLLEL
                     DPANDEAWHNVWRAFKAGGGA"
     gene            4014..5426
                     /locus_tag="VGP27_09525"
     CDS             4014..5426
                     /locus_tag="VGP27_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7851587.1"
                     /translation="MASSVVSGEKEGAVADTVNQLISAEEQVFLRRQPRSSELIAEAR
                     VHLAGGATSNWQIAEPQAVWMSHGVGSKVYDVDGNCYVDMHGGYGAAIAGHAHPAIVA
                     AVSDRVRRGTHFAQPTEDAIWIACELARRFELPLWRFANSGTEATMDAVHLARALTGR
                     DLIIKVEGCYHGHHDSVQVSVLPEADEVGPRDRPNAVPGNSGIPAAIRDLVIVVPFND
                     TGAVARALAEHRGQVAAMILEPVMMNAGIIPPDDGYLAVIRDLLHREGAFLIYDEVKT
                     GFTTGPGGITALSGVVPDMVCLAKALGGGISVAAIGGTETVMSAIADGRYEQVGTFNG
                     NPLAMAATRATLTDVLTPQSYAHLDELATRLRTSLEATIARHGFDWHVVTAGAKGCVT
                     FRRDPVREFRDFLHIDARLGHLHWLMQHNGGVFLPPWGKVEQWLLSVQHDRDDVDRFA
                     DNFARFADSISRHAAVAVAP"
     gene            5440..6615
                     /locus_tag="VGP27_09530"
     CDS             5440..6615
                     /locus_tag="VGP27_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851588.1"
                     /translation="MKGGAIRLEALVKSFDGVPAVTGIDLDIPAGQFYSLLGASGCGK
                     TTTLRMIAGFEKPDSGRIELDGRDVAGDPPHKRPVNTVFQTYALFPFMTVWDNVAFGL
                     KYQKASKEDTKRRVGEALELARMDQFAKRRPAQLSGGQQQRVALARALVLRPKVLLLD
                     EPLGALDAKLRKQLQLELRAIQREVGITFVYVTHDQEEALTMSDQIAVLAEGRIEQVG
                     PPQEIYSAPATTFVAGFLGAANIFDADVLEADNGSAVCSALDTRLGAAVEGGATPGEA
                     AIVIRPERISVQGADEPVEQGHNAIAGVVSQVVYLGHCTQVHVDVGAPTALIVEVPNH
                     SGPGSVSHRPGERVNCVCTHDAVRVLHRSSAQVITDPVAEDLGARVERDDSGVLSVP"
     gene            complement(6602..>7153)
                     /locus_tag="VGP27_09535"
     CDS             complement(6602..>7153)
                     /locus_tag="VGP27_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851589.1"
                     /translation="RGFGDTRIADVAKRAGVSSALVIYYFGTRDRLLVDALRYSEESG
                     YEAMEQKLGEISSLRDRLSLLIKWTCVPEADNEIPGAWGLWFDLWAQAFRHDEVKAGR
                     VELDARWRAMIVDAVKSADLDTDIDIRTFALEFSALLDGLSIQVALDDPEVDSSLAYD
                     VAMRFAERELNLPAEKQRSVKER"
BASE COUNT         1237 a         2298 c         2417 g         1201 t
ORIGIN      
        1 cgggtgggcg gcgacctcgg acatccgggc cagtgcgccg atgcgaagcc gcgacggctg
       61 gaaatcgatg tcgcggtagg gcagcgcgtt gatgtcgacg atcgcttcgg gtcgttcgac
      121 ggtctcgcgc atcaagtcga cgagtgtggt gccgcctgcg atgtagcggc cgccggatgc
      181 cgcggccgtc aatgccgacg cctcgtcgga cgccttggtg aaccggaacg ggtacatgcg
      241 ctacgtggcc cgcgccgcgc cggccaccgc cgtcacgatg ttggtgtagg cgccgcaacg
      301 gcagatgttg ccgctcatcc actcgcggat ctcggcggat gagccggtgt ggccctcgga
      361 gatgcagccg acgccggaga cgatctgtcc cgacgtgcag tacccgcact ggaacgcgtc
      421 ggtgtcgatg aacgcctgct gcaacggatg aagccggccg ttgcgttcga ggccttcgat
      481 ggtggtgatc tcggcgccgt cgtgcatcac cgcgagcgtc aggcacgagt tgatccgcct
      541 gccgtcgagc aggatcgtgc acgcgccgca ggcgccctgg tcgcagccct tcttcgtgcc
      601 ggtcagccca gcgcgttcgc gcagcatgtc cagcagggag gtgcggttgt cgacggtgat
      661 ctcgcggtct tcgccgttga ttcggagtcg gacggtcgat gactgtgcgt cgccgctgtg
      721 ttcgtcctct ccgcagccgg cgatcagtgg gcttgccgcc accaccccgc cgaccgcgat
      781 ggacccaccg acgaacgttc ttcggttgat atccggcacc tcgcccaggg taggcatcca
      841 ccctgtgagt cgctactacg cctctcctgt gaagcccccg agtccgcggt cggctgtgtc
      901 gtcgccgcgt gtcatgcgcc ggtagatgaa aaagccgatg acgacggcgg ccagcgcggc
      961 caccagcagc agggtggcaa gggcgttgag cgcgggcgtc ggtgctgcgc gggcggtgtt
     1021 gtagatcttg accgaaaccg gctcggtggc ggcgttgccc gacagatagc gcaccagcac
     1081 gaagtcgtcg atcacgtcgg cgaagaccag cacggtgctc gcgaagatcg caggcagcag
     1141 catcggcacg atgatccgcc gcagcgcgcc gagcggcgac gcaccgagat ccattgcggc
     1201 ttcctcgtat tgcgggccga tggttgccag ccgggcccga accagcacag cagggtagga
     1261 cacctggaaa gtgacgaggc cgattacctg cgcggtggtt ccaaggtcga cgggcaccgc
     1321 caccgtcgtg atgacgaaca gcaacgcgac ggcgagcagc acttcgggca acacgaacga
     1381 cagcagcatc aggaagttcg cacccgcggg caaccggccg cgccagcggt cgatgccgat
     1441 cgcgaacaaa gtgccaagcg ggacggtgat cagcgtcgct atcacgccca gcttgatggt
     1501 ctgcaacagt gcggtgtgca gcgcgtcgtt gtgccatacc gacagggtct gatcgcccca
     1561 gtaccaccgg aacgagaagc cctgccacga cgtgcgggag cggccgtcat tgaacgagta
     1621 cgcgaccgcg atgagcaccg gcaacagaga ccacgccagg tatccgacgg tgatcgcgac
     1681 cagaaacctc ggcggtcgcc atgggttgtt ccaccactcc agcgggcctc tcacgttgac
     1741 acctcgcccg gtttcgacgt gacccggatg tagtagagca tcggcagcag cgcggcgagg
     1801 aacaccagca tgacgaatgc gcccgcctga cccgtctgac cgggtgactg cacgctgtcg
     1861 ttgatcaggt tgccgaccat cgttgtcttc ggcgaggccg agagcatgtc gctggtgaag
     1921 tagtcgccga gcatcggcag gcaggtcagc agtacggcgg cgacgattgt cggacggcac
     1981 agcggcaggg ttacccgcca gaacgacgaa atccggtcgg cgccgaggtc acgcgacgcc
     2041 tcgagcatcg gctgtgacag ccggtccagg cccgcgtaga gcggcaggat catgtacggc
     2101 acatagccgt acaccagccc gaggatgacc acgacgggct ggccggtcag ccacttcacg
     2161 tcgggggtga agaaaccgcc gaagcccaag accctgttga gcaggccgtc gtcctgtaag
     2221 aggttgaccc acgcgaacat tcgcatcatg tagctgatcc agaacggcgc gatcaacagc
     2281 gcaagcagca ggcccttgcg cttgccggag agccgggcga cgtagtaggc gaccgggaat
     2341 gcgatcagca gacacagcga actggcgacg atgacgaaag tgaaggtgcg gacaatcgcg
     2401 gccccgtaca caccgttgct gccgacgatg tgggtcagta cgtaggtgaa ctgcgtcggg
     2461 tcccactgca gcgggttcca caccggcacg ggtgtacgga agatcgggtc gacttggccg
     2521 aacacgatgc acagcaccac gtacagggga aaaacgaaga acgccgccag ccagatgagt
     2581 ccgggtgcgg ccagccccga ccagagcctg cttcggcgtt ctcggggcgg tgatgccgtg
     2641 cgcgaaggtg cggcgactac cggggccacc attcaaatca ggccccgcct ccggccttga
     2701 atgcccgcca gacgttatgc cacgcttcat cattggccgg atccagttcc agcagccggt
     2761 agcccgcatc gaagtattcg ggcttgacga ccgccgactt caggttcggt gggatgaacc
     2821 cgctggacac cagtgagtcg gggttgatgt tgttctgggg cggctggtac ccggtctgcg
     2881 cgaagttctg ctgggcgact tcgggatcga gcaggtgatt gatgaacagg tgggccagca
     2941 ccgggttctt gccgccacga agcatgacga acaagtcgtt gtccaccaga ccttttccgt
     3001 cctctgggaa ccagtaccgc agtacctcgg ggctctgacc ctccggcatg taactcagtg
     3061 cgccgttgat gatgtcgccc gaccacatct gcgccagacc tatctggccc gcgggcatgt
     3121 cgctgtacat cgtgatcgtc accttcggcg acgtggcggc gacaagggcg ttgagctgat
     3181 ctcctacgag cttgaggtcg gcctccgacg aggtgttgac gtcggtctta cccgcgcgca
     3241 gcagcaccat cgccatcgcg gtgtgccagt cgtcgatgat cgcggtcttg ttcttgtact
     3301 tcggatccca tagggcgtca taaggattgg agagggcgcc gatgttttcc gggacctggt
     3361 cattgcgcca tccgataccg gtggtgtaga tgaagttcgg tgccgagtag cgccactcct
     3421 ggtcgtacca cggattggtg aaagtcggcc acacgttctt gatgttcggg atgtagctgt
     3481 ggttcagcgg acgggccagt cctccggtca ccaacttgct gatctggtcg taactcggga
     3541 agtagaggtc gtagtcgaca ttgccgccac ggatcttggt gagcgcctcg tcggtgtcgt
     3601 tgaaggtcga gatctgaacc ttggtcttgt attggtcctc gaacgacttg acggcgtcgg
     3661 ggctgaggta gtcggcgtag ttgtacacct gcagcgtcgc gttctgctcc ggtgcgaggc
     3721 cgtcgccgat cggcttgttg tcgtcgcgga tgtcccactt gaccggtttg tccggcgccg
     3781 cgatcgtcag cgtgggctgt gatgacgacg gtccggactt ggagcaggcg gccaggaaac
     3841 cgcccagtga cggagcggcc gcggccagta ttgcggcgcg tgtcaggaac tgtcgacggc
     3901 tcggactggg cactggcatc tcgggcctcc ccgttgattt cgcggcttgt cgctggaagg
     3961 actctcgcat agactgaatg ctcagtcaac cgttccgtac tggaggtttc accgtggctt
     4021 cgtcggtggt gtcgggcgaa aaagagggcg cagtggccga caccgttaac caattgattt
     4081 ccgccgagga gcaggtgttt ctgcggcgtc agccgcgcag ctccgaactc atcgccgagg
     4141 cccgcgtgca cctcgcaggc ggggcgacgt ccaactggca gatcgccgaa ccgcaggccg
     4201 tctggatgag ccacggcgtc ggctcgaagg tgtatgacgt cgacggcaac tgctacgtcg
     4261 acatgcacgg cggatacggg gccgccatcg ccgggcacgc gcatccggcc atcgtcgccg
     4321 cggtaagcga tcgggtaagg cgcggaacgc atttcgccca accgaccgag gacgccatct
     4381 ggatcgcctg cgaactggcg cgccgcttcg aactgccgct gtggcgattc gccaactccg
     4441 gcaccgaagc caccatggac gccgtccacc tggcccgtgc gctcaccggc agggacctga
     4501 tcatcaaggt cgagggctgc taccacggcc atcacgactc ggtgcaggtc tccgtcctgc
     4561 ccgaggccga cgaggtcggg ccgcgggacc ggccgaatgc ggtgccgggc aactccggga
     4621 ttcccgcggc gatccgcgac ctcgtcatcg tggtgccgtt caacgacacc ggtgcggtgg
     4681 cgcgggcgct ggccgaacac cgcggccagg tggccgcgat gatcctcgag ccggtgatga
     4741 tgaacgcggg catcatcccg cccgacgacg gctacctcgc cgtcatccgc gacctgctgc
     4801 accgcgaggg cgctttcctg atctacgacg aggtgaagac ggggttcacc acggggcccg
     4861 gcggaatcac cgccctcagc ggagtagtgc ccgacatggt gtgcctggcc aaggcgctcg
     4921 gcggcggtat ctcggtcgcc gcgatcggcg gcaccgagac ggtgatgtcg gcgatcgccg
     4981 acggccgcta cgagcaggtg ggcaccttca acggcaaccc gctggcgatg gcggcgacca
     5041 gggcgacgct caccgacgtc ctcaccccgc agtcgtatgc gcacctcgac gagctggcga
     5101 cccggctgcg cacctcgctg gaggcgacca tcgcgcggca cggattcgat tggcacgtcg
     5161 tcaccgccgg cgccaagggc tgcgtgacct tccgccgcga tccggtgcgc gagttccgcg
     5221 acttcctgca catcgacgcc agactgggcc acctgcactg gctgatgcag cacaatggcg
     5281 gcgtattcct gccgccatgg gggaaggtgg agcaatggct gctgtcggtt cagcacgacc
     5341 gcgacgatgt cgaccggttc gcggacaatt tcgcccggtt cgccgactcc atttcgcggc
     5401 acgccgcggt ggcggtcgcg ccttgacggg ccggcccgga tgaagggcgg cgcgatccgg
     5461 ctcgaggcgc tggtgaagtc gttcgacggg gtcccggcgg tcaccggtat cgacctcgac
     5521 atcccggccg gccagttcta ttcgctgctc ggcgcgtccg gctgcggtaa gaccaccacc
     5581 ctgcgcatga tcgcgggttt cgagaagccc gactccggtc gcatcgaact cgacggccgt
     5641 gacgtggcgg gcgatccgcc gcacaagcgg ccggtcaaca ccgtcttcca gacctacgcg
     5701 ctgttcccgt tcatgacggt gtgggacaac gtcgcgttcg ggctcaagta tcagaaggcg
     5761 tccaaggagg acaccaagag gcgcgtcggc gaggcgctcg agctggcccg gatggaccag
     5821 ttcgcgaagc gacggcccgc ccagctgtcc ggcggacagc agcagcgcgt cgcgctggcg
     5881 cgggcactgg tgctccgccc gaaggtgctt cttctcgacg agccgctggg cgcgctggat
     5941 gccaagctgc gcaagcagtt acagctggag ttgcgcgcca tacagcgcga ggtcggcatc
     6001 accttcgtgt acgtcaccca cgaccaggaa gaggcgctca ccatgagcga ccagatcgcc
     6061 gtgctggccg agggtcgtat cgagcaggtg ggcccgccgc aggagatcta ctccgcaccg
     6121 gcaacgacgt tcgtcgccgg attcctgggc gctgcaaaca tcttcgacgc cgatgtgctg
     6181 gaggcggaca acggttcggc agtgtgctcg gccctcgaca cccgtctcgg ggcggcggtc
     6241 gagggcggcg ccacaccagg cgaggccgcg atcgtcatca ggcccgaacg catctcggta
     6301 cagggcgccg acgagccggt cgagcagggc cacaacgcga ttgccggtgt ggtgtcgcag
     6361 gtggtctacc tcggtcactg cacccaggtg cacgtcgacg tgggagcgcc gaccgcgctc
     6421 atcgtggagg tgccgaacca ctcggggccc gggtcggtca gccaccggcc gggtgaacgt
     6481 gtgaattgtg tgtgcacaca tgacgcggtg cgcgtcctgc atcgcagctc ggcgcaggtg
     6541 atcaccgatc cggttgccga agacctcggc gcacgcgtgg agcgggacga ctccggcgtg
     6601 ctcagcgttc cttgaccgaa cgctgtttct cggcaggcag attgagctcg cgttcggcga
     6661 accgcatggc gacgtcatag gccagcgacg agtcgacttc cgggtcgtcg agcgccacct
     6721 ggatggacaa cccgtcgagg agcgcggaga actccagcgc gaacgtccgg atgtcgatgt
     6781 cggtgtccag gtcggcggac ttcaccgcat cgacgatcat ggctcgccag cgcgcatcga
     6841 gctctacccg gccggccttg acctcgtcgt gccgaaatgc ctgcgcccac aggtcgaacc
     6901 acaaacccca ggcgcccgga atctcgttgt ccgcctccgg aacgcaggtc cacttgatca
     6961 gtaacgacag acgatcacgc agtgacgaga tctcgccgag cttctgctcc atcgcttcgt
     7021 accccgactc ctcggagtag cgcagcgcgt cgaccaagag ccggtcgcgg gtgccgaagt
     7081 agtagatgac cagtgccgag ctgacgccgg cccgcttcgc gacgtcggcg atgcgggtgt
     7141 ccccgaagcc ccg
//
LOCUS       DASYLX010000387         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2649473,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000387 DASYLX010000000
VERSION     DASYLX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649473"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..636
                     /locus_tag="VGP27_09540"
     CDS             127..636
                     /locus_tag="VGP27_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7851590.1"
                     /translation="MSASDSRINEMRRPTQVAALDVFLVLLRRQHSEVLCALRGAVHF
                     AAWQWGLPSGKVDAGEDPYAALIRETREEIGLALPPRDVHPAGVVHARFPGEGPRVGF
                     VFVVDQDIAAHGEPRNTEPGKCDKIDWYPVDDLPAPFENYNRAALQLALGPGMLAMLE
                     RGVLVQPDT"
     gene            682..1629
                     /locus_tag="VGP27_09545"
     CDS             682..1629
                     /locus_tag="VGP27_09545"
                     /inference="COORDINATES: protein motif:HMM:NF022430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HEV7851591.1"
                     /translation="MNEDALASGREYLYLGITWVPGVPSLDLVEVESARHIRLPVVGR
                     RLAFRATARGKFCTGRYSFINRTGIEYRPCPGQEPATQSAQCAVCADLDEFHFVHNFH
                     QGGYVPPALIEYMKQPHWLYVATFADGSSKVGTATGERKRSRIDEQGAILASYVAQTR
                     DGRTVRILEDAVTRELAMTQYRRRVDKVAALAHPQPRETIAAAHRQATDRVLAMLAPA
                     GRDGSAKVEQESWHPPVEMALILESAPDGGWPQYQHDIRNGEHGFLIDACAGPALLVH
                     TVDEPDAQQYILDLGRMKGRRVLAGAFASPASVVQNALF"
     gene            1658..2509
                     /locus_tag="VGP27_09550"
     CDS             1658..2509
                     /locus_tag="VGP27_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851592.1"
                     /translation="MTTDKKAFAPSMVILTEEPRVLRPPDLSLDGILGRFPGLAKVPA
                     LGITGSTAAGWGNPYSDIDIYAFTDGECELPEDDSVESWRSTDPRGLNRINWIGRYDD
                     ALVDLKVWPLDAPRRALADFLDGNDPELIEISYMLQDLIYRLSISQPLIGDDFFAQTR
                     SLIATSTYPAALARQLKVNTENRLNDVAGQLQAGDGLGARMSAMSAAGFVTDCCLVLA
                     GNLCRSEKWLLRRLERTPACGITVSEYCSVVLDGPHPGESFTDCARRIANWVRATNIK
                     IESAVLG"
     gene            2506..2778
                     /locus_tag="VGP27_09555"
     CDS             2506..2778
                     /locus_tag="VGP27_09555"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEV7851593.1"
                     /translation="MTIADETYIRSLNLQVRRGEDGGLLVETYFGRYTLDRDAREVWR
                     FIDGRRTVRAVAEQVAEARGEDVASVSGGVEQFCQHLATLGLLERA"
     gene            complement(2803..>3232)
                     /locus_tag="VGP27_09560"
     CDS             complement(2803..>3232)
                     /locus_tag="VGP27_09560"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851594.1"
                     /translation="PRTETSENPHRTAIRRSAMFPHGNPQPITTGTPEIRTQESGAVD
                     PGSVRFDATACARTVPCRQFFIHWSQTTLPRTSVTPYSLRAQEQPTASTLPTWDEVEA
                     IGIGSESARQYAAGVLLDRVDRHGDLLGDLLARGQALPAE"
BASE COUNT          523 a         1057 c         1086 g          566 t
ORIGIN      
        1 gtgccgccgg tcggcctcgc cgctcccgac ggttgggcat cgcgggcgcg cccgggcacc
       61 ggccggaatc ggcctatgcc atagtgtccg agttgattcc gtccgaccgc agatctggag
      121 ttcgcgatgt ccgccagcga cagcaggatc aacgaaatgc gccggccgac gcaggtggcg
      181 gcgctcgacg tctttctcgt cctgcttcgc cgccagcact ccgaggtgct gtgcgcgctg
      241 cgcggtgcgg tccacttcgc ggcctggcaa tggggactgc cgagcggaaa ggtcgatgcg
      301 ggtgaggacc cgtacgcagc actcattcgc gagacgcggg aggagatcgg gctggcgcta
      361 ccgccccgcg acgttcaccc ggcgggcgtc gtgcatgcgc gtttcccggg cgagggacct
      421 cgcgttggtt ttgtgttcgt ggtcgaccag gacatcgccg cacacggcga accccgcaat
      481 accgagcctg gcaaatgcga caagattgac tggtatcccg tcgacgacct gcccgcgccg
      541 ttcgagaact ataaccgcgc cgcactccag ttggcgctcg gaccgggcat gttggcgatg
      601 ctggagcggg gcgtcctagt ccagccggac acctgagatg tcgggtcggc gaggctgggt
      661 tgaggaaggg tggcgagcgg cgtgaacgag gatgccctgg cgtccggacg tgagtacctc
      721 tacctgggga ttacctgggt tcccggagtc ccgtccctgg accttgtcga agtggaatcg
      781 gcgcggcata tccgcctacc cgtcgtcggc cggcgcctcg ccttccgcgc gacagcacgc
      841 ggcaagtttt gcacgggccg ctacagcttc atcaatcgga ccggcatcga gtaccggccg
      901 tgcccggggc aggagccggc cacccagtcg gcgcaatgtg cggtgtgcgc cgacctggac
      961 gagttccatt tcgtgcacaa ctttcaccag ggcgggtacg tcccgccggc tctgatcgag
     1021 tacatgaaac agccccactg gctctacgtc gcaacctttg ctgacgggtc gagcaaggtc
     1081 ggtaccgcca ccggcgagcg gaagcgttcc cgcatcgacg agcagggcgc gatcctggcg
     1141 tcgtacgtgg cccagacccg cgacggccgt acggtgcgca tccttgagga cgcggtcacc
     1201 cgtgagctgg cgatgaccca gtaccgccgg cgggtcgaca aggtcgccgc gctggcccac
     1261 ccgcagcctc gcgagacgat cgcggctgcc caccggcagg cgactgaccg tgtgctggcc
     1321 atgctggccc cggctggccg ggacgggagc gccaaggttg agcaggagtc ttggcacccg
     1381 cccgtcgaga tggccctcat acttgaaagc gcccccgacg gcggttggcc gcagtaccag
     1441 cacgatatcc gcaacgggga gcacgggttc ctcatcgacg cctgcgcggg accggccttg
     1501 ctggtgcaca ccgtcgacga gccggacgcg cagcagtaca tcctggacct cggccggatg
     1561 aagggacgac gcgttctcgc cggcgcattc gcgtcgcccg catcggtcgt acagaatgcg
     1621 cttttttgaa ccgccatacc aatttagggg atagccgatg accacggata aaaaggcgtt
     1681 cgcgccgtcg atggtgattc ttaccgagga gccccgggtg ctccggcccc ctgatctgtc
     1741 gctggatgga attctcggcc ggtttccggg gctggccaag gtacccgcct tgggcatcac
     1801 cggttccacc gcggcgggct gggggaatcc gtacagcgat atcgacatat acgcgtttac
     1861 cgacggggaa tgcgaactgc cggaggacga cagcgtcgag agctggcgca gtacagaccc
     1921 gcggggcctg aaccgcatca actggatcgg gcgctacgac gacgcgctgg ttgatctcaa
     1981 ggtttggccg ttagatgccc cccgccgcgc actggccgac ttcttggacg gaaacgatcc
     2041 agaactcatt gagatcagct acatgttgca ggacctcatc taccgcttgt caatctccca
     2101 gccgctgatc ggcgacgact tctttgcgca gacgcgttcg ctcatcgcga cgtcgacgta
     2161 cccggctgct ctcgctcggc agctgaaggt caacaccgag aatcggctca acgacgtggc
     2221 cggccagctg caagcgggcg acggcttggg tgcccggatg tctgcgatgt ccgccgccgg
     2281 gttcgtcacc gactgttgcc tggtcctggc cggtaatctc tgccgcagtg agaaatggtt
     2341 gcttcgccgg ctcgaacgca cgccggcctg cggcataacc gtgagcgaat actgctccgt
     2401 cgtgctcgac ggcccgcatc ctggcgagtc cttcacggac tgcgcccggc ggatcgcgaa
     2461 ttgggtacgt gccaccaaca tcaaaatcga gagcgcggtg ctcggatgac aatcgccgat
     2521 gagacctaca tacgatctct caatctccag gttcgccggg gcgaggacgg cggtctcctc
     2581 gtcgagacct atttcgggcg ctacaccctc gatcgcgacg cgcgggaggt gtggcggttc
     2641 atcgacggcc gacggaccgt ccgggcagtc gctgagcagg tggccgaggc ccgcggcgag
     2701 gacgtcgcct ccgtcagcgg cggggtcgag caattctgtc agcacctcgc caccctcggg
     2761 ttgctcgagc gggcctgacc gtcgccccga tgagcacgag catcactcgg ctggcagcgc
     2821 ctggccgcga gccagtagat ctccgagcag atcgccgtgc cggtcgaccc ggtccagcag
     2881 cacaccggcg gcgtactggc gggccgactc cgatccgatc ccgatcgcct cgacttcgtc
     2941 ccaggtcggc agcgtcgacg cggtcggttg ctcctgcgcc cgcagcgagt acggggtgac
     3001 ggaggttctt ggcagggttg tctgactcca gtggatgaag aactgtcggc acggcacggt
     3061 cctcgcacac gctgtggcat cgaacctgac tgagcctgga tccaccgcgc ctgattcttg
     3121 agttctgatt tcgggcgtcc cggtggtgat tggctgcgga ttcccgtgcg ggaacatggc
     3181 ggatcgccgg atggccgtcc ggtgcgggtt ctccgaggtt tcggttcgtg gg
//
LOCUS       DASYLX010000388         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1663521,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000388 DASYLX010000000
VERSION     DASYLX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663521"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VGP27_09565"
     CDS             complement(<1..1113)
                     /locus_tag="VGP27_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7851595.1"
                     /translation="MDPGTLIREYLLLGLRFDRIEEGYVDSFTGDPALRETVASEPAP
                     DPADLARQADRLLAELAGVPRTNGSSAFDDARADYIGAHLRALACAGKKFAGQSVGFV
                     DEVDAYFDVQISKGDPDRYREAHVRLDEALGGTGPLDERMAAYRSAEEIPPERLEECI
                     HAFSSALRDRVRAEFPLPERETIDYEVVTDKPWSGFNYYLGDYRSTVAVNADLRQQMS
                     NLPRLVAHESYPGHHTEHCRKEAGLVAKRGHAEQTLFLVNTPQCLMAEGLADLALYVA
                     VGPKWGAWAREIYADLGLRFDGERAEAVSEATAALADVRQDAALMLHDEHRDVDDVVA
                     FLRRWLLVNDERARQSLRFLSSPLWRAYTSTYVEGYR"
     gene            1144..2082
                     /gene="coaA"
                     /locus_tag="VGP27_09570"
     CDS             1144..2082
                     /gene="coaA"
                     /locus_tag="VGP27_09570"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV7851596.1"
                     /translation="MPRLSEPSPYVEFDRTQWRALRMSTPLKLAEEELIKLRGLGEKI
                     DLLEVEEVYLPLARLIHLQVAARQRLFSATSEFLGEPHQNPDRPVPFIIGVAGSVAVG
                     KSTTARVLQALLARWEHHLRVDLVTTDGFLYPNGELTRRNLMHRKGFPESYDRRALMR
                     FVTAVKSGSDYACAPVYSHLLYDIVPDEKQVIRHPDILILEGLNVLQTGPALMVSDLF
                     DFSVYVDARIEDIEGWYISRFLSMRAGAFADPASHFHHYSTLTDDQAVFAASDIWHSI
                     NRPNLIDNILPTRPRATLVLRKDADHSINRLRLRKL"
     gene            complement(2101..2511)
                     /locus_tag="VGP27_09575"
     CDS             complement(2101..2511)
                     /locus_tag="VGP27_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851597.1"
                     /translation="MTAITTPRFKESTDSLLRFAMRADAVLTGLAGIAALPFAGWLAE
                     MAGTSKAFEYSMSAFFIAYGVVVLVLAQLPSVRKPGIAVITANLLYTVAAVAFVLSGW
                     MPLTTMGVVGTLATGIYTLVFAELQYQGVRRIKR"
     gene            complement(2606..3043)
                     /locus_tag="VGP27_09580"
     CDS             complement(2606..3043)
                     /locus_tag="VGP27_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851598.1"
                     /translation="MTAITTGPVRDARSDVAAGAAAGDSLLRFALRADATLCGALGLF
                     VAMAADPLSRLSGLSATSEWIAGAALVGYGAALYVAAGIRHVRRVGVGVFAGNVAFAV
                     LVVVVLTAGWLPLTALGVASTIAFTVVTLAFAYAQYLGVRRLA"
     gene            complement(3125..>4013)
                     /locus_tag="VGP27_09585"
     CDS             complement(3125..>4013)
                     /locus_tag="VGP27_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851599.1"
                     /translation="TLFFALGNAILVIPLGLVPSWLALASTGCDVVLLGVAVAMWDAF
                     DEGQALRGDMLRSFVATGVVVVLFGGQALVGMALTGEQTALTVLLFTSIAIAVSINVL
                     ADPLAGLLDRLAFSRSPGLRADRATLRRTEAALPLRSANPLSDIGDESFVRLTRRALG
                     HYGDLSKLVASPLTALPIIDERMAARGAPDQPLERANELRALLADRIARLKPRDGGDF
                     GTTEQWRHYNALYFPYVVGVRAYAQNATAAGLDPVARQAWQWFVTEVPQRSLHNWQNA
                     AARLIAADLRGNVVIAQDA"
BASE COUNT          701 a         1339 c         1356 g          617 t
ORIGIN      
        1 ccggtagccc tcgacgtagg tgctggtgta ggcccgccac agcggcgacg acaggaaccg
       61 cagcgactgc ctggctcgct cgtcgttgac cagcagccag cgccgcagga acgccaccac
      121 gtcgtcgacg tcgcggtgtt cgtcgtgcag catcagtgcg gcgtcctgcc tgacatcggc
      181 cagcgccgcg gtcgcctcgg acaccgcctc cgcccgttcc ccgtcgaagc gaaggcccag
      241 atccgcgtag atctcgcggg cccacgctcc ccacttcgga ccgaccgcga catagagcgc
      301 caggtcggcg agcccctcgg ccatcagaca ctgcggagtg ttgaccagga acagcgtctg
      361 ttcggcatgc cctcgcttgg ccaccaaccc cgcctccttg cggcagtgct ccgtgtggtg
      421 gcccggatac gactcatggg ccaccaagcg cgggaggttc gacatctgct ggcgcagatc
      481 ggcgttcacc gcgaccgtcg agcggtagtc gccgaggtag tagttgaatc ccgaccacgg
      541 cttgtcggtc accacctcgt aatcgatggt ctcgcgctcg ggcagcggaa actcggcgcg
      601 aacccggtcg cgcagcgcgc tcgagaacgc atggatgcat tcttcgagcc gttccggcgg
      661 gatctcctcg gcgctgcggt aggcagccat ccgctcgtcc agtggacccg tgccaccgag
      721 cgcctcgtcg aggcggacgt gcgcctcgcg gtagcggtcg ggatcgccct tgctgatctg
      781 cacgtcgaaa taggcgtcga cctcgtcgac gaaaccgacg gactgcccgg cgaatttctt
      841 tccggcacag gccagcgcgc gcagatgggc gccgatgtag tcggcgcggg cgtcatcgaa
      901 tgccgacgaa ccgttcgtgc gcggcacgcc tgccagctcg gccaacagcc ggtcggcctg
      961 ccgggccagg tcagcggggt cgggcgcagg ctccgacgcg acggtctcgc gcagcgcggg
     1021 gtcgccggtg aaggagtcga cgtagccttc ttcgatccgg tcgaaccgca ggccgagcag
     1081 cagatattcg cggatcaggg tgccggggtc cataacgacc acgctaactg caagactgac
     1141 gacatgccgc ggctgagcga gccgagcccc tacgtggagt tcgaccggac tcaatggcgt
     1201 gcgctgcgca tgtccacgcc gctgaagctg gccgaggaag agctgatcaa gctgcgcggt
     1261 ctcggtgaga agatcgacct gctcgaggtc gaagaggtct acctgccgct cgcccggttg
     1321 atccatctgc aggttgcggc acgccagcgg ctgttctccg ccacctcgga gttcctcggt
     1381 gaaccccacc agaacccgga ccggcccgtg ccattcatca tcggggtcgc gggcagcgtt
     1441 gcggtcggta agtcgaccac cgcgcgtgtg ctgcaggcgc tgttggcccg atgggagcac
     1501 catctgcggg tcgacctcgt caccaccgac ggcttcctgt acccgaacgg ggaactcacc
     1561 cgtcggaacc ttatgcaccg taagggtttt ccggagagct acgaccgcag ggcgctgatg
     1621 cggttcgtca ccgcggtgaa gtcaggatcc gattacgcct gcgctccggt gtattcgcat
     1681 ctgctctacg acatcgtccc cgacgagaag caggtgattc gacatcctga cattctcatc
     1741 ttggagggac tcaacgtgct gcaaaccggt ccggcgctga tggtttcgga cctgttcgac
     1801 ttctcggtct acgtcgacgc gcgtatcgag gacatcgagg gttggtatat ctcccggttc
     1861 ctgtcgatgc gcgccggcgc gttcgccgac cctgcctcgc attttcacca ctactccacc
     1921 ctgaccgacg atcaggcggt cttcgcggcg agcgacatct ggcattcgat caaccggccg
     1981 aacctgatcg acaacatcct gccgacgcgt ccgcgcgcga cgctcgtgct acgcaaggac
     2041 gccgaccact ccatcaaccg gctgcgtctg cgcaagctct agaggcgcga gcagtgaacg
     2101 tcagcgcttg atacgtcgca cgccctggta ctgcagttcg gcgaacacca gcgtgtagat
     2161 accggtggcg agcgtgccga ccacacccat ggtggtcagc ggcatccaac cggacagcac
     2221 gaacgccacc gcggcgaccg tgtacagcaa gttggcggtg atcacggcga tgccgggctt
     2281 acgcacagac ggcagctggg ccaggacgag cactacaacg ccgtacgcga tgaagaaggc
     2341 gctcatcgag tactcgaaag ccttgctcgt gcctgccatt tcggccagcc agcctgcgaa
     2401 gggaagtgcc gcgatgccgg caaggccggt gagtacggcg tcggcgcgca tcgcgaagcg
     2461 cagcaatgag tcggtggact ccttgaaccg gggggtggtg atggcggtca tgctgttcct
     2521 ttctttgggg ggtttcactt cagccggtgc cggagtcgac gccgcaacct ctgtcagcgg
     2581 cgactccgga ccggtttgaa gggagtcacg ccagacggcg cactccgagg tattgggcgt
     2641 aggcgaaggc caacgtgaca acggtgaagg cgatcgtcga ggccacgccg agcgcggtga
     2701 gcggcaacca gcccgcggtc agcaccacga cgaccaggac ggcgaacgcg acgttgccgg
     2761 cgaacacacc gacgccgacg cgtcgcacat gccgtatgcc tgcggcgaca tacagcgcgg
     2821 cgccgtagcc gacgagcgcc gcgcccgcga tccattccga tgtggcggac agacccgaga
     2881 gccgcgacaa cgggtcggcg gccatggcga cgaacaatcc gagcgccccg cacagggtgg
     2941 cgtcggcccg cagtgcgaaa cgcaacaatg agtcacctgc agccgcaccg gcggcgacat
     3001 cagatcgagc gtcccggacg ggcccggtgg tgatggcggt catggcaccg atgttgcgcg
     3061 ccaacgcact gccagatcga cgcaagacgc tgccagctac tgccaaccgc aggtagaagc
     3121 ggtgttatgc gtcttgcgcg atgacgacgt tgccgcgcag atcggcggcg atgagccgcg
     3181 cggcggcgtt ctgccagttg tgcagcgacc gctgcggcac ctcggtgacg aaccactgcc
     3241 aggcctgccg ggcaacgggg tcgaggccgg cggcggtggc gttctgcgcg taggcccgca
     3301 cgccgacgac atacgggaag tacagcgcgt tgtagtgccg ccactgctcg gtggtaccga
     3361 agtcgccgcc gtcgcgaggc ttgagccggg cgatgcggtc ggccagcagg gccctgagtt
     3421 cgttggcccg ttccaatggc tggtcgggtg cgccgcgggc ggccatccgc tcgtcgatga
     3481 tcggcaatgc ggtcagcggg ctggcgacga gcttggacag gtcaccatag tggccgagcg
     3541 cgcggcgggt gagccggacg aagctctcgt cgccgatgtc cgagagcgga ttcgccgacc
     3601 gcagcggcaa cgcggcctcg gtgcggcgca gcgtcgcgcg gtcggcgcgc aggcccggcg
     3661 agcgggagaa cgcaagccgg tcgagcaggc cggccagcgg atcggccagc acgttgatcg
     3721 agaccgcgat cgcgatgctg gtgaacagca ggacggtcag tgccgtctgc tcgcccgtca
     3781 acgccatacc gaccagcgcc tgcccgccga acagcacgac cacgacgccg gtggcgacga
     3841 acgaccgcag catgtcgccg cgcagcgcct gaccttcgtc gaacgcgtcc cacatcgcga
     3901 cggcgacgcc gagcaggacg acgtcgcacc cggtggacgc gagtgcgagc caactgggca
     3961 cgaggccgag cgggatgacg agtatcgcgt ttccgagcgc gaagaaaagc gtg
//
LOCUS       DASYLX010000389         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3856867,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000389 DASYLX010000000
VERSION     DASYLX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856867"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGP27_09590"
     CDS             <1..256
                     /locus_tag="VGP27_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851600.1"
                     /translation="LDEGRTLHLHATDNGLGAAGEWSITGTDDGIAFTHEHGKGDVAL
                     RGPATDLLLAVVRRHPAADAGLEMFGDTAVWDGWLERTPF"
     gene            275..619
                     /locus_tag="VGP27_09595"
     CDS             275..619
                     /locus_tag="VGP27_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7851601.1"
                     /translation="MTTSEIATVLAWHDALNTKDFDTLAELSSDDIEFGDANGAGQGR
                     DALLQWARSLDTIVDVGRMYVHDGVVVTEHTGGGAWAFRVVHDDVTSVFRHADLHSAL
                     AATELTEKDLVG"
     gene            complement(585..1853)
                     /locus_tag="VGP27_09600"
     CDS             complement(585..1853)
                     /locus_tag="VGP27_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEV7851602.1"
                     /translation="MTHSHAHSHTSTGPSPLGPLAAKIVVGFLVAIGVAVVAGAALLW
                     PGQEKVDIPLPFQNAAGGAVSTEAGTVLSTGKADCGGPDVGAVITRNVTQAEAKDAPC
                     VHTVVTIDSGPNANAKTVLEFSTGPGQPTLAPGDHIRISRQVDQSGATTYAFYDYERT
                     WPLAVLAAAFALVIVAVARWRGLRALVGIVVAFAVLVVFLLPALRDGAPAIPVALVAS
                     AAILYAVIYLAHGVSLRTSAALLGTLTSLLVAAVLSWAAIALAHLTGLSEDQNNEIAA
                     YLGQVSITGLLLAGFIIGSLGVLNDVTVTQASTVFELAEVGDGSTRRGIFLSAMRVGR
                     DHIASTVYTLVLAYAGSALPLLLLFSVANRPLGDVLTSETVAIEIARSAVGGIALALS
                     VPLTTAIAAVLATPGGVATVSRRGPSRSVR"
     gene            complement(1942..>2202)
                     /locus_tag="VGP27_09605"
     CDS             complement(1942..>2202)
                     /locus_tag="VGP27_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7851603.1"
                     /translation="EIPGVSCVKPAGALYVFPRLDPEVYDIADDEQLVLDLLLQEKIL
                     VTQGTGFNWPTPDHLRIVTLPWARDLSSAIERLGNFLTGYRQ"
BASE COUNT          382 a          724 c          765 g          331 t
ORIGIN      
        1 gctggacgag ggccggacgc tgcacctgca cgccaccgac aacggtctgg gcgccgcagg
       61 ggagtggagc atcaccggca ccgacgacgg gatcgcgttc acgcacgagc acggcaaggg
      121 cgacgtcgca ctgcggggac cggcaacgga tctgctgctg gccgtggtgc gccgccaccc
      181 ggcggccgac gcgggcctgg agatgttcgg tgacaccgcc gtttgggacg ggtggctcga
      241 gcgcacaccg ttctgagtgg cgtacgtttc gttcatgacg acttcggaga tcgcaaccgt
      301 cctcgcctgg cacgacgcgc tcaacaccaa ggatttcgac actctggccg aattgtccag
      361 tgacgacatc gagttcggtg acgcgaatgg tgcagggcag ggccgcgacg cgctgctgca
      421 gtgggcccgg tcactcgaca caatcgtcga tgtgggccgg atgtatgtgc acgacggcgt
      481 cgtggtcacc gagcacaccg gcggcggggc gtgggcgttc cgggtggtac acgatgacgt
      541 cacgtcggtg ttccggcacg ccgacctgca ctccgcactc gccgctaccg aactgaccga
      601 gaaggacctc gtcggctgac ggtggctacc ccgccgggcg tggccagcac ggctgcgatc
      661 gcggtcgtca gcggcaccga cagcgccagc gcgatgccgc cgaccgccga acgggcgatc
      721 tcgatggcga ccgtctcact ggtcagcacg tcgccgagcg gccggttggc gacactgaac
      781 agcaacagca gcggcagagc gctgcccgca tacgccagca ccagcgtgta gacggtgctt
      841 gcgatgtggt cgcggccgac ccgcatcgcg ctgaggaaga tcccgcgccg ggtgctgccg
      901 tcgcccacct ccgccaactc gaacaccgtc gacgcctgtg tgacggtcac gtcgttgagc
      961 acgcccagcg agccgatgat gaatccggcc agcagcagtc cggtgatcga cacctggccc
     1021 aggtaagcgg cgatctcgtt gttctggtct tccgatagac cggtgagatg ggcgagcgcg
     1081 attgcggccc aggacaatac ggcggcgacc agcagcgagg tcaaggttcc cagcagtgcg
     1141 gcgctggtgc gcaggctcac gccgtgcgcc agataaatga ccgcatacaa gatggcggcg
     1201 gacgccacca acgcgaccgg aatcgcgggg gcgccgtcgc gcagagcggg cagcaggaac
     1261 accaccaaca ctgcgaacgc gacgacgatg ccgaccagtg cccgcaatcc gcgccatcgg
     1321 gcgacggcga cgatgacgag ggcgaacgcc gcggcgagca cggccagcgg ccaggtgcgc
     1381 tcgtagtcgt agaacgcgta ggtggtggct ccggactgat cgacctgccg gctgatgcgg
     1441 atgtggtcgc ccggcgccag cgtcggctga ccgggtccgg tgctgaactc cagcaccgtc
     1501 ttggcgttgg cgttgggtcc ggaatcgatc gtcaccaccg tgtgcacgca gggcgcatcc
     1561 ttggcctcgg cctgcgtgac gttcctggtg atcaccgcgc cgacgtctgg cccgccgcaa
     1621 tccgccttgc cggtcgacag cacggtgcct gcctcggtgc tcaccgcacc gccggcggcg
     1681 ttctggaagg gcagcggaat gtcgaccttc tcttggccgg gccacaacag ggcggcgccc
     1741 gccaccaccg cgacaccgat cgcgaccagg aagccgacga cgatcttcgc ggccaacggc
     1801 cccaacgggg acggaccggt cgacgtgtgt gagtgggcgt gggagtgcgt cacccgcacg
     1861 agggtagagg cagcacctga acgagccctc agtagtcggc catcaccatc gtttcgaaca
     1921 tctcccgcag aatcgctgcg ctcattggcg gtagccggtc aggaagttgc cgagtcgttc
     1981 gatggcgcta ctcaggtcac gggcccacgg cagggtgacg atccgtaggt gatccggtgt
     2041 gggccagttg aatccggtgc cctgggtgac caggatcttc tcctgtagca gcagatccag
     2101 gacgagttgt tcgtcgtcgg cgatgtcata gacctcggga tccagccggg ggaagacata
     2161 cagcgcgcct gcgggtttga cgcacgacac cccggggatc tc
//
LOCUS       DASYLX010000390         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_237321,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000390 DASYLX010000000
VERSION     DASYLX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237321"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VGP27_09610"
     CDS             <1..164
                     /locus_tag="VGP27_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7851604.1"
                     /translation="QDAGMLGGTQAALEYDVVDDFPLNDQEILVRHLHKVAVDWVEDY
                     RNVHRPVGV"
     gene            161..517
                     /locus_tag="VGP27_09615"
     CDS             161..517
                     /locus_tag="VGP27_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851605.1"
                     /translation="MTVSDNRTAEPRLPSAFAEFEVFAEKWCLATEPERWNARLTTPM
                     PDIRAFYDAFSPRFEEAIEYCDKFPLDDLPDDALNLLHLVYSMIMVSMAIEVFGQQKP
                     TDSADAVINRIGEPVP"
     gene            514..1359
                     /locus_tag="VGP27_09620"
     CDS             514..1359
                     /locus_tag="VGP27_09620"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7851606.1"
                     /translation="MSLLTINKLTDSVGAEVLDVDADRLARDDALGAAVLEALEDNGV
                     LVFPGLGLGPEAQVAFCRRLGEIDHSADGHHPVAGIYPVTLDKKKNSSADYLRGTFDW
                     HIDGCTPIEDTFPQMATVLSAKQVADSGGETEFANSYAAYDALSDGDKERFESMRVVH
                     SLEASQRRVTPDPSPEVLAKWRSRRTHEHPLVWTHRSGRKSMVLGASADYVVGMDRDE
                     GTALLGDLVDRATVADRVYAHTWSVGDTVIWDNRGVLHRASPYDPDSPREMLRTTVLG
                     DEPIQ"
     gene            1454..>2460
                     /locus_tag="VGP27_09625"
     CDS             1454..>2460
                     /locus_tag="VGP27_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851607.1"
                     /translation="MSEDLTSVDFFRDGRLTDDPYTFYRALRDKCPVSREDHYGVTMV
                     TGWQEAVDVYNDAETFSSCVSVTGPFPGFPVPLEGDDVTELIVKHRDEIPFSDQLPTL
                     DPPTHTNHRALLMRLITPKRLKENEDAMWQLADDILDDFLAPGEGEFIKGFAAPFTLR
                     VIADLLGVPEQDRPELLERLARGTHGSGLGNADKTLNKTPLEYLYEVFATYVEDRRRE
                     PRDDVLTGLATATFPDGTMPEVGDVVRVATNVFSAGQETTVRLLSTALKVMGDRPDIQ
                     RRLRDDRSLLSNFIEECLRIESPVKGDFRLSRVPTTVGDEALAAGCTVMVVNGAANRD
                     PR"
BASE COUNT          436 a          817 c          795 g          412 t
ORIGIN      
        1 tgcaggacgc cggcatgctc ggcggaacac aggcggcact cgagtacgac gtcgtcgacg
       61 actttccgct caacgaccag gagatcctgg tccgccacct gcacaaggtg gccgtcgact
      121 gggtcgagga ctaccgcaac gtgcatcgcc cggtcggggt gtgactgtgt ctgacaaccg
      181 cacggccgaa ccccggctgc ccagtgcctt cgccgaattc gaggtgttcg ccgagaagtg
      241 gtgcctggca accgaaccgg agcgctggaa tgcgcggctg accacgccga tgccggacat
      301 ccgcgcgttc tacgatgcct tctcgccgcg attcgaagag gcgatcgagt actgcgacaa
      361 gttcccgctc gacgatctgc ccgatgacgc gctgaatctg ctgcacctcg tctactcgat
      421 gatcatggtc tcgatggcca tcgaggtgtt cggccagcag aagcccaccg actccgccga
      481 cgccgtcatc aatcgaatag gagagccggt gccatgagcc tgctgaccat caacaagctc
      541 accgactcgg tcggcgccga ggttcttgac gtcgacgccg accggctcgc gcgcgacgac
      601 gcgctcggtg cagcggttct cgaggcgctc gaggataatg gcgtactggt gtttccgggg
      661 ctcggcctcg gacccgaagc ccaggtggcg ttctgccgtc ggctcggcga gatcgatcac
      721 tccgccgacg gtcatcatcc cgttgcgggc atctacccgg tcacgcttga caagaaaaag
      781 aactcatcgg cggactacct gcgcggcacc ttcgattggc acatcgacgg ctgcaccccg
      841 atcgaggaca cgttccccca gatggccact gtgctgtcgg cgaagcaggt cgccgactcc
      901 ggcggcgaga ccgagttcgc caactcgtac gccgcctacg atgcgttgag cgacggcgat
      961 aaggaacgct tcgagtcaat gcgggtcgtg cattcgctgg aggcctccca gcgccgagtc
     1021 acccccgacc cgtcgccgga ggtgctggcg aagtggcggt ctcgccgcac ccatgagcat
     1081 ccgctggtgt ggacgcaccg aagcgggcgc aagtccatgg ttctcggcgc gtcggcggac
     1141 tacgtcgtcg gcatggaccg cgacgaaggc acggcactgt tgggggatct ggtcgaccgc
     1201 gccacggtcg ccgacagggt gtacgcccac acgtggtcgg tgggcgacac cgtcatctgg
     1261 gacaaccgcg gcgtcctgca tcgcgcttcc ccgtacgacc ccgactcgcc tcgggagatg
     1321 ctgcgcacaa ccgtcctggg tgacgaaccc attcagtagc ccgaccccct cgttgacggg
     1381 ggcggggcac tatggaaatc taatacccac agatgagaat cacgttctct tcagccgaga
     1441 tctgggagta gcaatgtcgg aggacctcac ctcggtcgac ttcttccggg acggccgtct
     1501 gacggacgat ccgtacacgt tttaccgggc acttcgcgac aagtgcccgg tcagccgcga
     1561 ggaccactac ggcgtcacga tggtgacggg ctggcaggag gccgtcgacg tctacaacga
     1621 cgcggaaaca ttctcgtcct gcgtttcggt gaccggtccg ttcccaggct ttccggttcc
     1681 gctcgagggg gacgatgtca ccgagctgat cgtgaagcac cgcgacgaga tcccgttcag
     1741 cgatcagctg ccgacgctgg acccaccgac tcacaccaac caccgtgcac tgctgatgcg
     1801 actgatcaca cccaagcgcc tcaaggagaa cgaggacgcg atgtggcagc tcgccgatga
     1861 catcctcgac gacttcctcg ctccgggtga gggtgagttc atcaagggtt tcgcggctcc
     1921 gttcacgctg cgcgtcatcg ccgacctgct cggcgtgccc gagcaggacc ggccggagct
     1981 cctcgagcgg ctggcccgcg gcacccatgg cagcgggttg ggcaacgcgg acaagacgtt
     2041 gaacaagacc ccgttggagt acctctacga ggtcttcgcc acctatgtgg aggaccgtcg
     2101 ccgcgagccg cgtgacgacg tgctgaccgg tttggcgacc gccacgttcc ccgacggaac
     2161 gatgcccgag gtgggcgacg tcgtgcgggt ggcgaccaac gtcttctcgg cggggcagga
     2221 aaccacggtg cggctgctgt ccaccgcgtt gaaggtgatg ggggaccggc ccgacattca
     2281 gcgcaggttg cgcgacgacc gcagcctcct ttcgaacttc atcgaggagt gcctgcgcat
     2341 cgagagcccg gtcaagggcg acttccggct gtcgcgcgtg ccgacgaccg tcggcgatga
     2401 agccctcgcg gccggctgca ccgtgatggt ggtcaacggg gcggccaacc gcgacccgcg
//
LOCUS       DASYLX010000391         6327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_456774,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000391 DASYLX010000000
VERSION     DASYLX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456774"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VGP27_09630"
     CDS             complement(<1..468)
                     /locus_tag="VGP27_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7851608.1"
                     /translation="MPSTASSAAVAVRSDERLEVLFEELSELVGQRNAIDGRIVEIAA
                     EMDRDELCGATGARSIAALVAWKTGSSPANAHTIAAVARRAAEFPRCVQGLREGRLSL
                     DQVGVIAERAGQGSDEHYAALAEAATVSQLRTAIKLEPRPEPDPQPERQRSITK"
     gene            complement(662..799)
                     /locus_tag="VGP27_09635"
     CDS             complement(662..799)
                     /locus_tag="VGP27_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851609.1"
                     /translation="MQVVSPARSVDLLIAATAHAHSARLYTRNAEDFHGIEGSVDIVA
                     A"
     gene            complement(876..1256)
                     /locus_tag="VGP27_09640"
     CDS             complement(876..1256)
                     /locus_tag="VGP27_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV7851610.1"
                     /translation="MRAVLDTSVVIAAGVASLEGELAISAVTLAELHFGVLVAKHQKV
                     RAERLRRLLLLERTFDALPVDDPVAASYGQLAAAVVDAGRKPGVRSLDLLIAATAHAH
                     SARLYTRNPDDFTGLEGLLDIAVA"
     gene            complement(1253..1501)
                     /locus_tag="VGP27_09645"
     CDS             complement(1253..1501)
                     /locus_tag="VGP27_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016895288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV7851611.1"
                     /translation="METIGLRELRQNASELVRRVEAGEKIVITVAGRPSARLVPAAHR
                     TWRQWADVAELFAGPADPDWETDREGLGHNLRDPWARG"
     gene            1622..2497
                     /locus_tag="VGP27_09650"
     CDS             1622..2497
                     /locus_tag="VGP27_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7851612.1"
                     /translation="MTELAQALVGGILIGGLYVAISIGFSLSFGVLDVVDLAVGMWVV
                     LGAFAAVVASEKLKVDAFLLLPAVFVVFGVVGWLIAPLIYRVRTSKYALPALMGLAFT
                     FGLATLIRGGLLTLFGYTPRAVHTAFFSGNVQLLGVTAPAIRVAGFAFAVVATGLFLA
                     FLFYTRTGLAIRATAQSKENAGLMGVDVKRISSLVYAIYTGLTAMAGAMLGAIYAMTP
                     EVGLRYTLFAFFVVVLAGLGSVVGVLAAGLFLGVLQSLVTTYVGADYTLMVVFAVLFV
                     ALLLFPQGISRRGLA"
     gene            2494..3519
                     /locus_tag="VGP27_09655"
     CDS             2494..3519
                     /locus_tag="VGP27_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7851613.1"
                     /translation="MSRPASGGNPYLNLGVLAAVVAAMCLLPMMTSPYYIRVATGVAM
                     WAGIALSWNVICGYAGYISFGHVAFFGIGAYTTAILMQPDVDWNFWATLPVAAVIAGA
                     VAALVGWPALRLKGAYFAIATWALAEAVREFATVVDFTGGSGGLKTPIRLDDNFFFYT
                     MLGAAAIAYALCYLLLERSRFGFRVKAVRDNEAAARAQGINTTLVKIEAFVLSAMIPA
                     VLGGINAYWITFINPDSVLNTIITDQLVVMVLVGGLGHAWGPALGAAAMFLMQEQLRV
                     SYGETTAYIIVVGVLVMAVVLFLPDGLVSLGRRARRMRLVRQLLGRARDDVAPTQKQA
                     ETEEAAR"
     gene            3516..4229
                     /locus_tag="VGP27_09660"
     CDS             3516..4229
                     /locus_tag="VGP27_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851614.1"
                     /translation="MTVLAVDDLFRSFGGIRAVDGVTFGVEQAEIVGIIGPNGSGKST
                     LFNLLTGALKPDKGTISLFDKNVTRLAPYKIARTGLSRTFQIPALFVNMTVRENLWTA
                     AVQFDWDNATAAADAVLERLELGHVADDLANTLSGGQQRLLEMGRVLMQNPKIALLDE
                     VAAGVHPRLRQIMLDAIRTLRDDGTTFLVIEHDMELAQDICDRIIVMDAGKIVAQGSF
                     EEISHDPHVMEAYLGVSAE"
     gene            4226..4969
                     /locus_tag="VGP27_09665"
     CDS             4226..4969
                     /locus_tag="VGP27_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851615.1"
                     /translation="MSEPDGSGTRDGVITVDRVDAGYGSVQILHQVSMTARTGEVTCV
                     FGPNGCGKSTLLKAMAGVIDPWTGTITLDDADLTHLPSHRTLGHGLAMMPQGGGVFPQ
                     LSVRDNLRIGGYTLRSRGQLDGRIEELLQEFPRLRERYGVQAGQLSGGEQMILSIARA
                     LVLRPRFLLFDEPSAGLSPKLVGDVLVRATELAQRGVGVIMIEQNIREALRVADRMYV
                     LVAGRNRFEGTPADVEDDRELIHLYLGGR"
     gene            5023..6303
                     /locus_tag="VGP27_09670"
     CDS             5023..6303
                     /locus_tag="VGP27_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7851616.1"
                     /translation="MSNYINRRRFITRAGLVVGGAVAGPSLLAACGGDTGGGGASDKF
                     KVGAVLELSGESATGGQIAQRGYQLWADTVNKAGGLSIGDKKYQVDLVVQDCKSDPAT
                     GADATSRLVTEEGVNAIFGAYTSGVQIAMDPICAKYKVPCIAGSAESPNIWKSKPEFT
                     FGVIPAVDTTAARSIQAIVDNANPKPVTAAVVGANEPFSDNTAQGFRTGVQDAKLQLV
                     HYGLFPPNADLAPVAGAVAAQRPDIVAVGGHDILLVDFVKAMAASGYTPKAIIEHYGI
                     TDASFAKALGRQADGVLGISVWLPTATFRDGLFGSAGDYAKTFEQQHGSPPDYTAAGC
                     SAAGQVLQAAVEKLGEPPSLSEEARGKLNDLIAGTDLTTFYGPIKFASDGDHFHNNTA
                     LNPMLVQIQGGQVKAIAPPDSAQAKMIYPLPPLG"
BASE COUNT         1038 a         2110 c         2088 g         1091 t
ORIGIN      
        1 tttggtgatc gaccgctgac gttcgggttg cggatcgggt tccggtcgcg gttccagctt
       61 gatcgcggtg cgcagctggc tgaccgtggc ggcctcggcc agcgccgcat aatgctcatc
      121 agaaccctga cccgcccgct cagcgatcac gccgacctga tccagcgaca gccggccctc
      181 ccgcaaaccc tggacgcagc ggggaaactc cgccgcccgg cgcgcgacag cagcgatcgt
      241 gtgcgcgttt gccggagacg aaccggtctt ccacgccacc aacgccgcga ttgaccgcgc
      301 ccccgtagca ccacacagct catcgcgatc catctccgca gcgatctcca caatgcgtcc
      361 atcaatcgcg ttgcgctgcc caaccaactc cgacaactcc tcaaacaaca cctcgaggcg
      421 ctcatcagaa cgcaccgcaa cagcggcaga ggatgcggtc gaaggcatag tccaatcatt
      481 gcagccgggt acgacaactt ccggctgccc gatccgcgcc gggtgtggcc gacaccgcaa
      541 atcattttcg agctgcgggc cgtataggtt ggcagcgtct acagggcgag ccttgcgcct
      601 cggtcggcaa attcgcgcca cgcccagccg gggcgtaacg gtcggcagcc tcgcgctcgc
      661 gttaggcggc gacgatgtcg actgaccctt cgatgccgtg aaagtcttcg gcgttgcgcg
      721 tgtagagccg ggccgaatgc gcatgtgcgg tagctgcaat cagaagatcc accgagcgcg
      781 cggggctgac cacctgcacc gacgcgccgc gagctggcca tgactcgccg cgatcttttc
      841 tcggtggtcg cggaccccga cagccgtgac ggcaatcaag ccacggcgat gtcgagcagc
      901 ccttcgagcc cggtgaagtc gtctggattg cgcgtgtaca gccgcgccga atgtgcgtgc
      961 gcagtagccg caatcaggag atcgagagat cgcacccccg gcttacgccc ggcatcgacg
     1021 acagcggcgg caagctggcc gtagctcgca gcgacggggt catcgactgg tagggcgtca
     1081 aatgtgcgct cgagcagcag cagacgccga aggcgctcag cgcgaacctt ttgatgtttg
     1141 gcgacgagga caccgaaatg tagctccgcc aacgttacgg cgctgatcgc gagttcgcct
     1201 tcgagcgaag cgacgcccgc agcgataacg acactcgtat cgaggactgc tctcacccgc
     1261 gtgcccaggg atcacggagg ttgtgcccga gcccttctct gtcggtctcc caatcggggt
     1321 ctgcggggcc ggcgaacagt tcggccacgt ctgcccactg gcgccaggtc cggtgggcgg
     1381 cgggaacgag gcgggcactg ggccgcccgg cgacggtgat gacgattttt tcgccggctt
     1441 cgacacggcg gacgagctcg gacgcgttct gccgaagctc acgtagtccg attgtctcca
     1501 tgacgccacg gtagcacttg tgctacgtgc aaagccggga ccaaagcgat tgatccattg
     1561 tcaacaaacc tacggtccgg atacgctccg agcgtctcga ccgctctgcc gagaggactt
     1621 tgtgaccgag ctggcgcaag cgcttgtcgg ggggattctc atcggtggtc tctacgtggc
     1681 catcagcatc ggcttctcgt tgtccttcgg cgttctcgac gtcgtcgacc tcgcggtcgg
     1741 aatgtgggtg gtgctcggcg cattcgctgc ggtcgtggcc agcgaaaagc tgaaagtgga
     1801 cgcgttctta ctgctcccgg ccgtcttcgt ggtcttcggg gtcgtcggct ggctcatcgc
     1861 gccgctgatc taccgggtcc ggacgagcaa gtacgcacta ccggcgctga tggggctggc
     1921 attcaccttc gggctcgcca ccctgatccg cggcggcctg ctcacgctgt tcggctacac
     1981 gcctcgcgcg gtgcacacgg ccttcttctc cggcaacgtc cagctgctcg gcgtcaccgc
     2041 acccgcgatc cgggtggccg gtttcgcctt cgcagtcgtg gccaccggcc tgttcctcgc
     2101 gttcctgttc tacacccgca cgggcctcgc gatccgggcc accgcgcaga gcaaggagaa
     2161 cgccgggttg atgggcgtcg acgtgaagcg gatcagcagc ctggtgtacg ccatctacac
     2221 cggcctcacc gcgatggccg gcgccatgct cggcgccatc tacgccatga ccccggaggt
     2281 cggcctgcgc tacaccctgt tcgcattctt cgtcgtggtg ctcgccgggc tcggctcggt
     2341 ggtcggcgta ctggccgccg gattgttcct cggtgtgctg cagtcgttgg tcacgacgta
     2401 tgtgggcgcc gactacacac tgatggtggt cttcgcggtg ctgttcgtgg cgctgctgtt
     2461 gttcccgcaa ggcatctcgc gccggggcct ggcatgagca gacccgcctc ggggggcaac
     2521 ccgtacctga acctcggcgt gctcgctgcc gtcgtcgcgg cgatgtgtct gctgccgatg
     2581 atgacgtcgc cgtactacat ccgcgtcgcc accggcgtcg cgatgtgggc gggaatcgcg
     2641 ctgtcctgga acgtgatctg cggctacgcc ggctacatca gcttcggtca tgtggcgttt
     2701 ttcggcatcg gcgcctacac caccgcgatc ctgatgcagc ccgacgtcga ctggaacttc
     2761 tgggcgaccc ttcccgtcgc ggccgtcatc gcgggcgccg tcgcggcgct ggtcggctgg
     2821 ccggcattac gcctgaaggg cgcgtacttc gcgatcgcga cctgggcgct ggccgaggcc
     2881 gtccgcgaat tcgcaacggt cgtcgacttc accgggggtt ccggcggact caaaacgccg
     2941 atccgcctcg acgacaactt cttcttctac acgatgctcg gtgccgcggc gatcgcctac
     3001 gccctgtgct acctcctgct ggagcgatcg cgattcggtt tccgcgtcaa ggcggtgcgc
     3061 gacaacgaag ctgccgcccg cgcacagggc atcaacacca cgctggtcaa gatcgaggcg
     3121 ttcgtgctga gcgcgatgat cccggccgtg ctgggcggca tcaacgccta ctggatcacg
     3181 ttcatcaacc ccgacagcgt gctgaacacc atcatcaccg accagctcgt cgtgatggtg
     3241 ctcgtcggcg ggctcggcca cgcgtggggc ccggccctcg gtgcggcggc gatgttcctg
     3301 atgcaggagc agctccgggt cagctacggc gagacgaccg cctacatcat cgttgtcggc
     3361 gtgctggtga tggcggtcgt gctgttcctg cccgacgggc tggtcagcct cggccggcgg
     3421 gcgcgacgaa tgcggttggt gcgtcagctt cttggacgcg cccgcgacga tgtcgcaccg
     3481 acccagaagc aagccgagac agaggaggcc gcgcggtgac ggtgcttgcg gtcgacgacc
     3541 tgttccgctc cttcggcggc atccgcgcgg tcgacggtgt cacgttcggc gtcgagcagg
     3601 ccgagatcgt gggcatcatc gggccgaacg gcagcgggaa atccacgctg ttcaacctgc
     3661 tcaccggcgc gctgaaaccg gacaagggca ccatctcgtt gttcgacaag aacgtcacca
     3721 ggctcgcccc ttacaagatc gcccgcacgg gcctcagccg gaccttccag ataccggctc
     3781 ttttcgtgaa tatgactgtg cgggagaatc tttggaccgc ggcggtgcag ttcgactggg
     3841 acaacgcgac ggcggcggcg gacgccgtgc tggagcggct ggagctcggt cacgtcgccg
     3901 acgacctcgc gaacaccttg tccggcggac agcagcgact gctggagatg gggcgggtgc
     3961 tgatgcagaa cccgaagatc gccctgctgg acgaggtcgc cgccggtgtc catccgcggc
     4021 tgcgccagat catgctggac gccatccgta ccctgcgtga cgacggcacg acgtttctgg
     4081 tgatcgagca cgacatggaa ctcgcgcagg acatctgcga ccgcatcatc gtgatggacg
     4141 ccggaaagat cgtggcgcaa ggcagtttcg aggagatctc gcacgatccc cacgtgatgg
     4201 aggcgtatct gggggtatcg gcggagtgag tgaacccgac ggcagcggca cacgggacgg
     4261 cgtgatcacc gtcgaccgcg tggacgcggg atacggcagc gtgcagatcc tgcaccaggt
     4321 gtcgatgacc gcccgcaccg gcgaggtcac ctgcgtcttc gggccgaacg gctgcggcaa
     4381 gagcaccttg ctgaaggcga tggccggagt tatcgaccct tggacgggca cgatcacgct
     4441 cgacgacgcc gatctcaccc acctgccgtc tcatcgcacg cttggtcacg ggctcgcgat
     4501 gatgccgcag ggcggcggcg tgttccctca gctctcggtg cgcgacaacc tgcgcatcgg
     4561 cggctacacg ctgcgctccc ggggccagct cgacgggcgg atcgaggagc tgctgcagga
     4621 gttcccgcga ctgcgcgagc gctacggtgt gcaggccgga cagctttccg gcggtgagca
     4681 gatgatcctc tcgatcgccc gcgcgctcgt cctgcgccca cggtttctgc tgttcgacga
     4741 gccgtcggca ggcctgtccc ccaagctggt cggcgacgtc ctggtccgcg cgaccgagct
     4801 ggcccagcgc ggcgtgggcg taatcatgat cgagcagaac atccgcgagg cgttgcgggt
     4861 ggccgaccgc atgtatgtcc ttgtggcggg gcgcaatcgg ttcgaaggca cacccgcgga
     4921 cgtcgaagac gaccgcgagc tcatccatct gtatctaggg ggtcgctgaa cgatgcaccg
     4981 cttcggcccg ctgcccgcat gggcaactcg agaggagaaa tgatgtccaa ctacatcaac
     5041 cgtcgacgct tcatcaccag ggccggcctc gtggtgggcg gcgcggttgc cggcccgagc
     5101 ctgctggccg cgtgcggcgg cgacacgggc ggtggcggag catcggacaa gttcaaggtc
     5161 ggcgccgtgc tcgaactgtc cggcgaatcc gcgacgggcg gccagatcgc ccagcgtgga
     5221 taccagctgt gggccgacac ggtgaacaag gcaggcgggc tgtccatcgg ggacaagaag
     5281 tatcaagtcg atctggtcgt gcaggactgc aaaagcgatc ccgccaccgg cgccgacgcg
     5341 acgtcccggc tcgtcaccga ggagggcgtg aacgccatct tcggtgcgta caccagcggc
     5401 gtgcagatcg cgatggaccc gatatgcgcc aagtacaagg taccgtgcat cgccggctcg
     5461 gcggagtccc ctaacatctg gaagagcaag cccgagttca cctttggcgt catccccgcc
     5521 gtcgatacca cggcggcccg ctcgatccag gcgatcgtcg acaacgcgaa tcccaaacct
     5581 gtgaccgcgg cggtcgtcgg cgccaacgag ccgttctccg acaacaccgc ccagggcttc
     5641 cgcacggggg tacaggacgc gaagctccaa ctcgtgcact acgggctgtt cccgccgaac
     5701 gccgacctgg cgccggtggc cggcgcggtc gcggctcaga ggcccgacat tgtggcggtc
     5761 ggcggccacg acattttgct tgtcgacttc gtgaaggcga tggccgcttc gggttacacg
     5821 cccaaggcga tcatcgagca ctacgggatc accgatgcct ccttcgcgaa ggcgctcggc
     5881 cgtcaagccg acggcgtttt ggggatctcc gtttggctgc cgaccgcgac tttccgcgac
     5941 ggcctgttcg gctcggcagg cgactacgcg aagacgttcg agcagcagca cggctcgcca
     6001 ccggattaca ccgccgctgg gtgcagcgcc gccgggcagg tgctgcaggc cgcggtcgag
     6061 aagctcggtg agccgccgtc gctgtcggag gaggcccggg gcaagctgaa cgatctgatc
     6121 gccggcaccg acctgacgac cttctacgga ccgatcaagt tcgcatcgga cggcgaccat
     6181 ttccacaaca acaccgcgct caacccgatg ctggtccaga tccagggcgg gcaggtcaag
     6241 gccatcgcgc cgccggactc ggcgcaggcg aagatgatct atccgcttcc gccgctgggc
     6301 tgagcgcagt cagcggcgtg tgccaac
//
LOCUS       DASYLX010000392         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_346638,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000392 DASYLX010000000
VERSION     DASYLX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346638"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGP27_09675"
     CDS             complement(<1..291)
                     /locus_tag="VGP27_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7851617.1"
                     /translation="MIFSADGAAAFQGRAGTLSCPADQNLLRDLRAFADVVLVGAGTA
                     RAEQYGPARFTAAQEALRGADRGFAPPPIAVVSQTGHLPPTLFVEAASTPILV"
     gene            complement(369..869)
                     /locus_tag="VGP27_09680"
     CDS             complement(369..869)
                     /locus_tag="VGP27_09680"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7851618.1"
                     /translation="MSGSEAFEEIVAALNYPMFVVTTKTTDRDEALAGCLVGFASQTS
                     IHPPRFLVGLSRRNHTFRTAQHSTHLAVHVLSRKHLALAQLFGGETGDEVDKFARCAW
                     EAGPTGLPILTDASAWFVGRILERYDLGDHVGHLLEPINGSASGMSGDWVSFADVRDL
                     EPGHEA"
     gene            complement(923..>3130)
                     /gene="treS"
                     /locus_tag="VGP27_09685"
     CDS             complement(923..>3130)
                     /gene="treS"
                     /locus_tag="VGP27_09685"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009269 - response to desiccation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEV7851619.1"
                     /translation="HLHPARPRSLRFRPRVRTPFARRSLTQDGTPTGDNPAYVSWLLS
                     QSMLADANEISQQFSGQGSMWQNPYATPSPRGAVETASVWFTAYPLSLITRPDESFLK
                     AMADENMWKAFAEVGIEAVHTGPVKRAGGLSGWEQTPSVDGHFDRISTQIDPAFGTED
                     DFRSMCGTAGWYGGTIIDDIVPGHTGKGADFRLAEMKYADYPGIYHMVEVDPRDWENL
                     PDVPSGVDSVNIDAATEEWLDRAGYIIGRLQRVIFYAEGVKVTNWSVTRPVVGVDGVE
                     RRWVYLHYFKDGQPSINWLDPSFAGMRLVIGDALHSLADLGTGGLRLDANGFLGAEKT
                     AAENSTAWSEGHPLSEAANHLVASMVRKVGGFTFQELNLTIDDIREIGQAGADLSYDF
                     INRPAYHHALATADTEFLRLTLRTTLELGVDPASLVHALQNHDELTYELVHWSTGHRD
                     DMYTYKGEEITGGDLGETIRSDLTEKLTGENAPYNLVFTTNGIACTTATVIGATLGIE
                     DLDAIEDVDRVRKAHLLLAMFNALQPGVFALSGWDICGMLTLNPSDVTELLRGGDTRW
                     IHRAAHDLMGVNPSASQSMAGIPRGRSLYGAIPDQLSDDTSFLRQLQAILKVRSHYGI
                     ATSRQIDIPEVSHRAMLVLVHQLADERQYQLTVLNFAGEEVSGTVRSEKLPASSTVSD
                     MFTGKALATVDDLHSFSVDLPAYHGMSLLVEAPVEADENHYQSGSASTSIAQT"
BASE COUNT          559 a          947 c         1012 g          612 t
ORIGIN      
        1 cacgaggatg ggggtcgatg cggcttcgac gaacaacgtc ggcgggagat gtccagtctg
       61 gctgacgact gcgatcggtg gtggcgcgaa cccgcgatct gctccacgca gcgcttcctg
      121 agccgcagtg aaacgtgccg gaccgtactg ctcggcgcgc gcggtgcccg ctccgaccaa
      181 gacgacgtcg gcgaaagcac gcaggtcgcg gaggaggttt tggtcggcgg ggcaagagag
      241 tgtgccggcg cggccctgga acgccgcagc accgtcggcg ctgaagatca tgttcgcgcg
      301 cacaccgttg ggtgcgtcgg catagagccc tgccagatca cacgggtcct caatgctcgt
      361 gaggtccgtc acgcttcgtg ccctggttcg aggtcgcgta cgtcggcgaa tgaaacccaa
      421 tcaccagaca tacctgacgc actgccattt atgggttcca gcagatggcc aacgtggtcg
      481 cccaggtcgt accgctcgag gatgcggccg acgaaccatg ccgaggcgtc agtcagtatc
      541 ggcaagcccg tggggccggc ctcccaagcg caccgggcga acttgtcgac ttcgtcgcct
      601 gtctcacctc cgaaaagttg tgcgagcgcc aaatgtttgc gggacagcac atgtacggct
      661 agatgcgtgg aatgttgcgc tgtgcggaac gtgtggtttc gcctggaaag gcccacgagg
      721 aaccgaggag ggtgaatgct ggtttggctg gcgaagccaa ccagacagcc tgcgagggct
      781 tcatcgcggt cggttgtttt ggtggtgacc acgaacatcg gatagttgag tgccgcgact
      841 atctcctcga aggcttctga tccgctcatg ggtccgagga tagggctagc cgatccgctg
      901 accacctgag atccaccgca cttcaggtct gtgcgatcga tgtcgacgct gaccccgatt
      961 ggtagtggtt ctcgtcagcc tcgaccgggg cctcgaccag aagcgacatg ccgtggtatg
     1021 caggcaagtc aaccgagaag ctgtgcagat cgtccacggt ggcaagggct tttccggtga
     1081 acatgtccga gacggtgctg ctcgcaggca gcttctccga gcgcaccgtt ccactgactt
     1141 cctcgccggc gaagttcaac accgtgagct ggtactgccg ttcgtcggcc aactggtgga
     1201 cgagaaccag catggcgcgg tgcgacacct cgggtatgtc gatctggcgg ctggtagcga
     1261 tcccgtagtg cgagcgcacc ttaaggatgg cctgcagctg cctgagaaaa ctcgtgtcat
     1321 cggagagctg gtcgggtatc gcgccgtaaa gacttctgcc gcgcggtatt ccagccatcg
     1381 attgactcgc ggacgggttg acgcccatca ggtcgtgtgc cgcgcggtga atccatcgcg
     1441 tgtcgccgcc gcgcaagagt tcagtcacgt ccgacgggtt caacgtcagc atcccgcaga
     1501 tgtcccatcc cgacagggcg aatacaccgg gctgtagcgc gttgaacatc gccaacagga
     1561 ggtgggcttt tcggacgcgg tcaacgtctt cgatcgcgtc caggtcctcg ataccgagcg
     1621 ttgccccgat caccgtggcc gtggtgcatg cgatcccatt ggtggtgaaa accaagttgt
     1681 acggcgcatt ttcgccggtg agcttctccg tcagatccga gcggattgtc tcaccaagat
     1741 cccctccggt gatttcctct cccttgtagg tgtacatgtc gtcgcggtga cccgtcgacc
     1801 agtgcacgag ttcgtaggta agctcatcgt ggttctgcag tgcgtgcacc agagaggccg
     1861 gatcgactcc cagttccagc gtggtgcgca gagtcagcct caaaaactcc gtgtccgccg
     1921 tcgccagggc gtggtgatag gcgggtcgat tgatgaagtc ataggacagg tccgcaccgg
     1981 cttgtccgat ttcgcggatg tcatcgatgg tgaggttcag ctcctgaaaa gtgaagccgc
     2041 ccaccttgcg caccatgctg gcaaccaggt ggttggccgc ctccgacagg ggatgaccct
     2101 ccgaccacgc ggtactgttc tcggcggcgg ttttctctgc tccaagaaag ccgttggcgt
     2161 ccaagcgaag accgccggtg cccaggtccg ccagcgaatg cagggcgtcg ccgatgacga
     2221 ggcgcatccc ggcgaaggaa gggtccagcc agttgatcga cggttgtccg tccttgaagt
     2281 agtgcaggta cacccatcga cgctcgaccc cgtcgacgcc gacgacaggc cgcgtaacgc
     2341 tccagttggt cactttgacc ccttcggcgt agaagatgac ccgctgcagt cggccgatga
     2401 tgtaaccggc cctgtcgagc cactcttccg ttgctgcgtc gatgttcacc gaatcgacgc
     2461 cggacggaac gtctggcagg ttttcccagt cacgaggatc gacctcgacc atgtgataga
     2521 tgccggggta gtccgcgtac ttcatttcgg ccagccggaa gtccgcgccc ttacccgtgt
     2581 gtcccggcac gatgtcgtcg atgatcgtcc cgccgtacca acctgccgtt ccacacatgc
     2641 tgcggaagtc gtcctcggtc ccgaatgccg ggtcgatttg tgtgctgatg cggtcgaagt
     2701 ggccgtccac gctgggggtc tgttcccatc cggaaagccc gcccgcccgt ttcaccggtc
     2761 cggtgtggac ggcttcgata ccgacctcgg cgaaggcctt ccacatgttc tcgtcagcca
     2821 tcgctttgag gaacgactcg tcgggccggg tgatcagtga caggggataa gcggtgaacc
     2881 acaccgaagc ggtctcgacg gcgccacgcg gactgggcgt ggcgtagggg ttctgccaca
     2941 tcgagccctg cccggagaat tgctggctga tctcgttggc gtcggccaac atcgactggc
     3001 tcagcagcca tgacacatag gccggattgt ctcctgtggg tgtcccgtcc tgggtgaggg
     3061 agcggcgggc gaacggtgtg cgtacccggg gccggaatcg cagcgaacgc ggccgtgcgg
     3121 gatgaaggtg
//
LOCUS       DASYLX010000393         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_896522,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000393 DASYLX010000000
VERSION     DASYLX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896522"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGP27_09690"
     CDS             complement(<1..236)
                     /locus_tag="VGP27_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7851620.1"
                     /translation="MTNSPDTGDRPVLLEVRDVVVHYGRIQALHGVSLVVRQGELVTL
                     LGSNGAGKTTIMRAISGLRPLTSGSVWFEGEEISR"
     gene            complement(220..1074)
                     /locus_tag="VGP27_09695"
     CDS             complement(220..1074)
                     /locus_tag="VGP27_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851621.1"
                     /translation="MTTPIQGVEEIVEELAGVHREIHAAEGEVLLQTQDLTVKFGGLT
                     ALDSVTFDIRRGEILGMIGPNGAGKTTCFNAITGVYRPSSGSVIFDGAPLGRIKRHQI
                     TRRGIARTFQNIRLFGEMTALENVMVGTDARHHTSVPGALVRTPRHRREERSAVERSV
                     ALLHFVGIAHRGEEKAKNLPYGDQRRLEIARALATEPKLLCLDEPAAGFNPSEKAALI
                     DLIRKIRDDGYTVLLIEHDMRLVMGVTDRIVVLEFGRKIADGLPAEIREDPKVIAAYL
                     GVPDDELS"
     gene            complement(1071..2252)
                     /locus_tag="VGP27_09700"
     CDS             complement(1071..2252)
                     /locus_tag="VGP27_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7851622.1"
                     /translation="MSDTRAKRLLAPGDGLRQWWDGLSRVQKWMFGVLLFGAVALLPL
                     YTPPFLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGYVGFYAVGAYTVALL
                     TSPDSPWNKVEPNGFLSAPWAWLSCVPLAMAFTALAGLILGIPTLRLRGDYLAIVTLG
                     FGEIIRLMADNLSDITNGPRGLNEVAFPRVGESEKLPEGLFSNANSTGHANYGTWWFW
                     LGLILIVAILLLVGNLERSRVGRAWIAIREDEDAAEVMGVNTFRFKLWAFVIGAAIGG
                     LSGALYAGQVQYVAPPTFNIINSMLFLCAVVLGGQGNKLGVIFGAFIIVYLPNRLLGV
                     EFLGINLGDLKYLFFGLALVVLMIFRPEGLFPARQQLLAYGRAARDLLRSGPREKEPA
                     A"
     gene            complement(2249..>2759)
                     /locus_tag="VGP27_09705"
     CDS             complement(2249..>2759)
                     /locus_tag="VGP27_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7851623.1"
                     /translation="PKTQFTIFGGSVSNVTLVIIVAALVLAVLTDVAINRTKFGRGIR
                     AVAQDPTTATLMGVSRERIIMTTFLIGGLLAGAAALLYTLKVPQGIIYSGGFLLGIKA
                     FSAAVLGGIGNLRGALLGGLLLGIMENYGQAVFGTQWRDVIAFALLVMVLLFRPTGIL
                     GESLGKARA"
BASE COUNT          511 a          928 c          877 g          443 t
ORIGIN      
        1 cgggagatct cctccccctc gaaccacacc gatcctgacg tcagcggccg cagccccgaa
       61 atcgctcgca tgatcgtggt cttgcccgca ccgttggaac cgagcagggt caccaactcg
      121 ccctgccgca ccaccaggga aacgccgtgc agggcctgga tgcggccgta gtgcacgacg
      181 acgtcgcgga cctcgagcag gacgggccgg tcgccggtgt caggagagtt cgtcatcggg
      241 cacccccagg taggcggcga tgaccttcgg gtcttcgcgg atttcggcgg gcagtccgtc
      301 ggcgatcttc cggccgaact ccagcaccac gatccggtcg gtcaccccca tgaccaatcg
      361 catgtcgtgc tcgatgagca gcaccgtgta gccgtcgtcg cggatcttgc ggatgagatc
      421 gatcaacgcg gccttctcgc tcggattgaa tccggcggcc ggctcgtcga gacacaacag
      481 cttgggttcg gtcgcgagcg cacgcgcgat ctcgaggcgc cgctggtcgc cgtacggcag
      541 attcttcgcc ttctcctcgc cgcggtgcgc tattccgacg aaatgcagca gcgcgaccga
      601 acgctcgacc gccgagcgtt cctcacgcct gtgtcgcggc gtgcgcacga gcgcaccggg
      661 caccgaggtg tgatgtcgcg catccgtgcc caccatgacg ttctccagcg cggtcatctc
      721 gccgaacagc cggatgttct ggaaggtgcg ggcgatcccc ctgcgggtga tctggtgccg
      781 cttgatgcgc ccgagcggcg ccccgtcgaa gatcaccgag ccggaactgg gccggtagac
      841 gccggtgatc gcgttgaagc acgtcgtttt acccgctccg ttggggccga tcatgccgag
      901 gatctcgccg cgccgaatgt cgaacgtcac cgaatccagt gcggtcagtc caccgaactt
      961 gacggtcaaa tcctgtgtct gcagcaggac ttcgccttct gcggcgtgaa tctcacgatg
     1021 gacgccggcc aattcctcga cgatttcctc tacaccttgt atcggcgtgg tcatgcggcc
     1081 ggctccttct cgcgcgggcc cgacctcagc aggtcgcgcg cggccctccc gtaggccagc
     1141 agctgctggc gggccgggaa cagaccctcc ggccggaaga tcatcagcac cacgagcgcg
     1201 agcccgaaga acaggtattt caagtcgccg aggttgatgc cgaggaactc gacacccagc
     1261 aggcggttgg gcaggtagac gatgatgaac gcgccgaaga tcacaccgag cttgttgccc
     1321 tgcccgccca gcacgaccgc gcagaggaac agcatcgagt tgatgatgtt gaaggtcggc
     1381 ggcgcgacgt actgcacctg tccggcgtac agggctccgg atagtccgcc gatcgcggcg
     1441 ccgatcacga acgcccacag tttgaaccga aaggtgttga cgcccatgac ttctgcggca
     1501 tcctcgtcct cccggatggc gatccacgca cggccgacgc ggctgcgctc caggttgccg
     1561 accagaagca ggattgccac gatgaggatc agcccgagcc agaaccacca ggtgccgtag
     1621 ttcgcatggc ccgttgagtt ggcattggag aaaagaccct cgggaagctt ctcgctctcc
     1681 cccacccgcg ggaaggcgac ctcgttcagc ccacgcgggc cgttggtgat gtctgaaagg
     1741 ttgtcggcca tcagccggat gatctcgccg aaccccagtg tgacgatcgc caggtagtcg
     1801 ccgcgtaacc gcagcgtcgg gataccaagg atcaggcccg cgagtgcggt gaatgccatc
     1861 gccagcggca cgcaggacaa ccaagcccac ggggcgctga gaaacccgtt cggttcaacc
     1921 ttgttccacg gactgtccgg gctggtgagc agcgcgacgg tgtaagcacc gacggcgtag
     1981 aagcccacgt agcccaggtc cagcagccct gcctggccca ccacgacgtt gagcccgatc
     2041 gcgatgatcg cgaccatggc gaactgcgcc atggttccgc cgaagctgat gcccggcgtg
     2101 tcgaggaacg gcggtgtgta cagcggcaac agagcgacgg cgccgaacag caggacgccg
     2161 aacatccatt tctggacccg ggacaggccg tcccaccact ggcgcaggcc gtcgccgggc
     2221 gccagcaggc gtttcgccct tgtgtcgctc acgcgcgtgc cttcccgagg ctttcaccga
     2281 gtatcccggt gggccggaac agcagcacca ttacgagcag cgcgaaggcg atcacgtcgc
     2341 gccattgagt gccgaagacg gcctgcccat aattctccat gatgcccagc agcaggccgc
     2401 cgagcagcgc accgcgcagg ttgccgatgc caccgagcac ggcggccgag aaggccttga
     2461 tacccaacag gaatccgccg gagtagatga tgccctgggg aaccttcagc gtgtacagca
     2521 gcgcggcagc gcccgccagc aggccgccga tcaagaaggt cgtcatgatg atgcgctcgc
     2581 gcgagacacc catcaacgtg gccgtggtcg ggtcctgtgc gacggcgcga atgcctcggc
     2641 cgaacttcgt gcggttgatc gcgacatcgg tcagcaccgc caagaccaac gccgccacga
     2701 tgatgaccag ggtgacgttg gacaccgagc caccgaagat ggtgaactgc gtcttcggc
//
LOCUS       DASYLX010000394         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3638243,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000394 DASYLX010000000
VERSION     DASYLX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638243"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..>2772)
                     /locus_tag="VGP27_09710"
     CDS             complement(160..>2772)
                     /locus_tag="VGP27_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851624.1"
                     /translation="MATNPPAFPNRLHDEFWIVVPAAVATVKWCALIVAMCAVPAAIF
                     AVWRLMVSYISRRVRRKNGKEWWDRALSVPQPAPRSDQGDAENSWRQAYYVPGADDLV
                     TVIDGPAVKVPRTPSALCLSGGGIRSGCVAMGAMQTLSRRPEGDEPAPWGGAPMLDGF
                     DYVISVSGGGYTSGARLMAIQKADEGGPAVENGRALTLADRFSPGSPEFAFLRSRSSY
                     IADSPIGLVRALAEVFKNLVLSVLMLVLVAVIFGWVFGWFVARLPLAAFVPSRVGTPP
                     GAVHLPALDHHLTVAATAVAIPVVCALALGGLALLCEYATTRRSVTVFKDRLNLAASA
                     VTLLAVVVFAVTMALPALMWLCAPLSPSADNKAPPVAAFGGFAAVLILQYGATIISMV
                     TKKKEESGEGRASRIRKMLPPGVVQLLIVLLTLTVLVIAWLLVVGIAAAQVFGWLTRN
                     GDSLRPIPHWLWILLAIAFVVTILSCVDVTSLSLHPFYRGRLARAFAVRRIGGEAQGY
                     KAEEGTWLDNYGRTGEGGPKFVFAAAAALSGDAKPAPGLNAVSFVLSADYIGGPALGW
                     LDTSETIAISSSRIKRDLTVQAAVAISGAAFASAMGRMNKGFQSLLAVSGARLGTWLP
                     NPAFVKNAKDNASQPLYPKALPSVRGAGYFYRELFGINRKQARLVQVTDGGHYENLGL
                     VEALRRRCRLIYVIDGGGDTPPLLSGLSDAIRLARFELGVEVELDERGDYGVPNLAPG
                     SAEPFGAGHAFHSLNSRVTAGAVVRGVIKYPAAAGMGHHNVGLLIVAKAVLWQDLPDW
                     VLTYGAGKSGESFPNDKTSDQWFNEAQFAAYTEVGRRIAVKARTVRSHEEQPVPPKAP
                     ARESMLVEADGQGS"
BASE COUNT          495 a          978 c          877 g          422 t
ORIGIN      
        1 gtgctcggcc tcggacgaga gcagatcttc ctgcttgtcg ttcctccaca gaccgaacca
       61 gacgacgcac atcacacgat gatgaccgcg gccgcaccgg gcaacgattc gacggtcgac
      121 gacctgctgg cgaccgatgg gcctagaggc tgagccgcgt cagctgccct ggccgtcggc
      181 ttcgaccagc atgctctcgc gtgccggcgc cttgggcggt accggttgct cctcgtgtga
      241 gcgcaccgtg cgcgccttga ccgcgatgcg ccgtccgacc tcggtgtagg ccgcgaactg
      301 cgcctcgttg aaccattggt cggacgtctt gtcgttcggg aagctctcgc cgctcttgcc
      361 cgcgccgtac gtcagaaccc agtcgggaag gtcctgccac aacacggcct tggcgacgat
      421 cagcagtccg acgttgtggt ggcccatgcc cgcagccgcc gggtacttga tgactccacg
      481 caccaccgcg cctgcggtga ccctgctgtt gagcgagtgg aacgcgtgcc cggcgccgaa
      541 cggttccgct gatcccggcg ccaggttcgg gacgccgtag tcgccacgct cgtcgagttc
      601 gacctccacg ccgagctcga atcgggccag ccggatggcg tccgacaagc cggacagcag
      661 cgggggagtg tcacctccgc cgtcgatgac gtagatcagc ctgcaccggc gccgcagcgc
      721 ctcgacgagc cctaggttct cgtagtggcc gccgtcggtc acctgcacca gacgcgcctg
      781 cttgcggttg atcccgaaca gctcccggta gaagtagccc gccccgcgca ctgacggcaa
      841 cgcctttgga tagagcggtt gtgacgcatt gtctttcgcg ttcttgacga aagccgggtt
      901 cggcaaccac gttcccaggc gtgctcccga caccgccagc agcgactgaa aacccttgtt
      961 catccgcccc atcgccgacg cgaacgccgc accgctgatc gcgaccgcgg cctggacggt
     1021 caggtcgcgt ttgatgcgcg acgacgagat cgcgatcgtc tcgctggtgt ccagccatcc
     1081 gagcgcgggg ccgccgatgt agtccgcgct caacacaaac gagaccgcgt tgagccccgg
     1141 cgcgggcttg gcatcaccgg agagcgccgc cgccgcggcg aacacgaact tcggcccacc
     1201 ctctccggtg cgaccgtagt tgtccagcca cgtcccctcc tcggccttgt agccctgcgc
     1261 ctcgccaccg atccgacgca cggcgaaggc tcgtgccaac cgtccgcgat agaacgggtg
     1321 caggctcagc gatgtgacgt cgacgcagct gaggatcgtg acgacaaacg cgatggcgag
     1381 caagatccac agccagtggg ggatcggccg aaggctgtcg ccgttgcggg tcagccagcc
     1441 gaagacctgt gcggccgcga tgcccaccac caggagccac gcgatgacca gcacagtcaa
     1501 cgtcagcagc acgatcagaa gctgaaccac accgggcggc agcatctttc gaatgcgtga
     1561 ggcgcggcct tcgccggact cctccttttt cttggtaacc atcgagatga tggtcgcgcc
     1621 gtactgcagg atcaacacag cggcgaagcc gccgaaggcc gcgacgggcg gggccttgtt
     1681 gtccgcgctc ggcgacagcg gcgcgcacag ccacatcagc gccggcagtg ccatcgtgac
     1741 ggcgaacact acgaccgcga gcagggtgac ggcactcgcg gccaagttga gtcggtcttt
     1801 gaacaccgtg accgaccgcc gcgtggtggc gtattcgcag agcagtgcga gcccgccaag
     1861 cgccaatgcg cacaccaccg gtatcgcgac ggcggtcgcg gccaccgtga ggtgatggtc
     1921 cagcgcgggc agatgcacgg cgcccggcgg cgttccgacc cgcgacggca cgaacgcagc
     1981 caagggcaag cgtgccacga accagccgaa cacccagccg aagatcaccg ccacgagcac
     2041 cagcatcagc accgacagca cgaggttctt gaacacctcg gccagcgccc tgaccaatcc
     2101 gatcggtgag tcggcgatgt aactggatct gctgcgaaga aaggcgaatt ccggtgagcc
     2161 cgggctgaac cggtcggcaa gggtgagcgc tcggccgttc tccacagcgg ggccgccttc
     2221 atcggccttc tggatcgcca tcagcctggc acccgaggtg taaccgccgc cggagaccga
     2281 gatgacatag tcgaacccgt cgagcatcgg ggcgccgccc cacggcgccg gctcgtcccc
     2341 ttcaggccga cgcgacaagg tctgcatggc acccatcgcc acgcaacccg atcgaatccc
     2401 cccacccgac aggcacaagg cgctgggtgt tctcggcacc ttcacggccg gaccgtctat
     2461 cacggtgacg aggtcgtcgg ctcccggtac gtaatacgct tggcgccatg agttctcggc
     2521 atcgccctga tcggaccggg gggccggctg cggcacactg agcgccctgt cccaccactc
     2581 cttgccgttc ttgcggcgca cccggcgaga tatgtaggac accatcaacc tccagacggc
     2641 gaatatcgcc gcgggcaccg cgcacatcgc gacgatgagc gcgcaccact tcaccgtcgc
     2701 gaccgcggcg ggaacgacga tccagaattc gtcgtgcagc cggttgggaa aggcaggcgg
     2761 attcgtcgcc at
//
LOCUS       DASYLX010000395         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2211909,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000395 DASYLX010000000
VERSION     DASYLX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211909"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VGP27_09715"
     CDS             <1..96
                     /locus_tag="VGP27_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEV7851625.1"
                     /translation="APTTEGARGSDVIVEGIAEGVTITLDEFTQS"
     gene            93..521
                     /locus_tag="VGP27_09720"
     CDS             93..521
                     /locus_tag="VGP27_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV7851626.1"
                     /translation="MSTSVVAVIAVLIAAFGVAYVINRLIALRAGLKDTAVSAGSRVV
                     DTSDLGLSGTGPTVLHFSAAWCGPCSGVRRVVDEVCKELPQVAHVEIDMDANPDAARR
                     LSVLSLPTTFIFDADGRQRYRAAGVPTAADLRSALDPLLA"
     gene            738..1211
                     /locus_tag="VGP27_09725"
     CDS             738..1211
                     /locus_tag="VGP27_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEV7851627.1"
                     /translation="MGSPTKTNAPVDQVDVRGPRYAAWVTTAVLVLTLIVSSISLAAA
                     AVILGVQAAIFAIGAVGGPRKHPYGLIFAKLVAPRLAPVTEKEPVPPLKFAQLVGFVF
                     AVVGSVGFAAGFGTLGLIATGFALVAAFLNAAFAICLGCQLYPLVARLRRSAAPA"
     gene            1248..2081
                     /locus_tag="VGP27_09730"
     CDS             1248..2081
                     /locus_tag="VGP27_09730"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV7851628.1"
                     /translation="MARSDVLVSADWAESNLDAPNTVFVEVDEDTSAYDGGHIAGAVK
                     LDWKTDLQDPVKRDFVDAQQFSKLLSDRGIANDDTVILYGGNNNWFAAYAYWYFKFYG
                     HDQVKLLDGGRKKWELDGKPLSTDAVNRPATSYTAKQPDNSIRAFRDEVIDAIGDKNL
                     VDVRSPDEFSGKILAPAHLPQEQSQRPGHIPSAINVPWSKAANEDGTFKSDDDLATLY
                     AEAGLDGEKETIAYCRIGERSSHTWFVLQEILGHKNVKNYDGSWTEYGSLVGAPIELG
                     S"
     gene            2083..2385
                     /locus_tag="VGP27_09735"
     CDS             2083..2385
                     /locus_tag="VGP27_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HEV7851629.1"
                     /translation="MCSAPKQGQTLPAGVDLEKETVITGRVVDGSGQTVGGAFVRLLD
                     SSDEFTAEVVASATGDFRFFAAPGTWTLRALSPAGNGDASVAPSGAGIHEVDVKVA"
     gene            2680..3336
                     /locus_tag="VGP27_09740"
     CDS             2680..3336
                     /locus_tag="VGP27_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024636948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEV7851630.1"
                     /translation="MTSDEGSVAGGSGDRAVAAAAERAKVTAARNIPVFSDLPAPADT
                     ANLREGANLNDALLALLPLVGVWRGEGEGRAAQGDYRFGQQIVVAHDGGDYLIWEARS
                     WRLTESGEYEKPGLRESGYWRFVDDPNDLSESQAIELLLAHSAGYVELFYGQPRSQSS
                     WELVTDALARSKSGMLVGGAKRLYGIVEGGDLAYVEERVDADGGLVPHLSARLTRFVG
                     "
     gene            3336..3599
                     /locus_tag="VGP27_09745"
     CDS             3336..3599
                     /locus_tag="VGP27_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851631.1"
                     /translation="MRRVRLAAALAAAVIIAGCSSNQSPSEESSTQSPAPSGHGSYAS
                     CLHEHGVSAPPGPVAGPPPGTDEATWHKATQACASLAPGPDAG"
     gene            complement(3964..>4344)
                     /locus_tag="VGP27_09750"
     CDS             complement(3964..>4344)
                     /locus_tag="VGP27_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV7851632.1"
                     /translation="RHAERQGAGDVIFISSEGRVLEGPRSTVVIAIEEDGRPCLLTPP
                     PWYPILRGTTQQALFEVARNKGYDCDYRALQPGDLFAAQGVWLVSSITLAARVHTLDG
                     RALPPSPMAAELTELVDAAIVSDR"
BASE COUNT          715 a         1437 c         1438 g          754 t
ORIGIN      
        1 gctccgacca ccgaaggcgc ccgcggatcc gacgttatcg tcgagggcat cgccgaggga
       61 gtaaccatca cgctggatga gttcacacag tcatgagcac ttcagttgtc gcggtcatcg
      121 ccgtgctcat agcggcgttc ggggtggcct atgtgatcaa caggttgatc gccctgcgtg
      181 ccggactgaa ggacaccgcc gtgtcagccg gctcaagggt cgtcgacacc tccgacctgg
      241 gtttgtccgg caccgggccg accgtcctgc atttcagcgc ggcgtggtgc ggaccgtgtt
      301 ccggggtgcg gcgggtcgtc gacgaggtgt gcaaggagct gccccaggtg gctcatgtgg
      361 agatcgacat ggacgccaat cccgacgcgg cccggcgcct ttcggtgctg tccttgccta
      421 cgaccttcat cttcgacgcc gacggccggc agcggtaccg ggccgccggc gttccgaccg
      481 ccgctgacct gcggtccgcg ctcgacccgc tgttggcttg atcaccatgt cattgggtaa
      541 gctggccggc gtgtccgccc gcctgttggt gttgctcacc aagcgccgcg cagtcgatct
      601 gtgccgcgtc gcgggctcct gttgttgttg tcgtagctgc tgagcgcaga cgcgcgacct
      661 agcgcgctcg ctctgagcag tccgatgagg cctgctcgca ccaagcccta ccaacgcaac
      721 acaggagtgt gatttcgatg ggatcgccca ccaagaccaa tgcaccggtc gaccaagtcg
      781 acgttcgggg accccgctac gccgcatggg tcacgacggc ggtgctggtg ctcacgctga
      841 tcgtctccag catcagcctc gccgccgccg cagtgatcct cggcgtacag gctgcgatct
      901 tcgcgatcgg tgcggtgggc gggccgcgga aacatcctta tgggctcatc ttcgcgaagc
      961 tcgttgcgcc ccggctcgca cctgtcacgg agaaggagcc ggtgccgccg ctgaagttcg
     1021 cccagctggt cggattcgtc ttcgccgttg tcggcagcgt cgggtttgcg gccgggttcg
     1081 gcacgctggg gttgatcgcc accggcttcg cgttggtcgc cgcgttcctc aacgcggcct
     1141 tcgccatctg tctcggctgt cagctctacc ccctggtggc gcggctccgt cgctccgccg
     1201 cccccgcgta agacctcgaa tatcaagcaa ccgaaaggat tttcgccatg gctcgctccg
     1261 atgtcctggt ctcagccgac tgggccgaga gcaatctcga cgcgccgaac accgtcttcg
     1321 tcgaggtcga cgaagacacc tccgcctacg acggcggaca catcgcaggt gccgtaaagc
     1381 tggactggaa gaccgacctg caagatccgg tcaagcgcga tttcgttgac gcacagcagt
     1441 tctcgaagct gttgtccgac cgcggcatcg ccaacgacga caccgtgatc ctctacggcg
     1501 gcaacaacaa ctggttcgcc gcatacgcgt actggtactt caagttctac ggccacgacc
     1561 aggtcaagct tctcgacggt ggtcgcaaga aatgggagct cgacggcaag ccgctttcca
     1621 ccgatgcggt gaaccgtccg gcgaccagct acaccgccaa gcagcccgac aacagcatcc
     1681 gcgcgttccg cgacgaggtc atcgacgcca tcggcgacaa gaacctcgtc gacgtgcgtt
     1741 cgcccgacga gttctccggc aagatcctcg cccccgcgca tctgccgcag gaacagagcc
     1801 agcggccggg ccacatcccc agcgccatca atgttccgtg gagcaaggca gccaacgagg
     1861 acggcacctt caagtccgat gacgacctgg ccacgctgta cgccgaggcc ggactggacg
     1921 gcgagaagga aaccatcgcc tactgccgca tcggtgagcg ctcgtcgcac acctggttcg
     1981 tgctgcaaga gatcctcggc cacaagaacg tcaagaatta cgacggcagt tggacggaat
     2041 acggctccct ggtgggggcc ccgattgagt tgggaagttg atatgtgctc tgcaccgaag
     2101 caaggacaga cgctgccggc cggcgttgac ctggaaaagg aaacggtgat caccggtcgc
     2161 gtcgtcgacg gctccggtca gaccgtcgga ggcgcgttcg tgcggctgct ggactcgtct
     2221 gacgagttca ccgctgaggt cgtcgcgtcg gccaccggtg acttccggtt cttcgccgca
     2281 cccggcacgt ggacgctgcg tgcgctgtcc cccgcgggca acggcgacgc gagcgtcgca
     2341 ccgtcgggcg caggaatcca cgaggtcgac gtcaaggtcg cctgacgacg aaggcggcgg
     2401 cagccgacta ggagtcgggc aattgaacct tcgcctgacg acgaaggcgg cggcagccga
     2461 ctaggagtcg ggcaatcgca cctcgcctga cgacgaaggc ggcgcaggcg cagcaggcgg
     2521 cagcctcgca ccgatcagcc gactgacatc aacctcatga ctcggtacaa ctagactcgc
     2581 tgccgtggtg ttgatctacg aactcctgct tgtggcgacc gtcgtggtca tcacttggtt
     2641 cgcgctctat gcgctgtatc gactcattac cgacgaatcg tgacttcgga cgaaggctcc
     2701 gtcgccggcg gttcgggcga ccgggcggtt gcggccgccg ccgagcgggc caaggtgacg
     2761 gcggcgcgaa acatcccggt gttctccgac ttgcccgccc ccgccgacac cgcgaacctg
     2821 cgcgaaggcg cgaacctgaa cgatgcgctg cttgctctgc tgccgctggt cggtgtgtgg
     2881 cgcggcgagg gtgagggccg agcagcccag ggcgactatc ggttcggcca gcagatcgta
     2941 gtggcccacg acggcggcga ctatctgatc tgggaagcgc ggtcgtggcg gctgaccgag
     3001 tcgggcgaat acgaaaagcc cggcctgcgg gaatcgggct actggcggtt cgtcgacgat
     3061 ccgaacgacc tctccgaatc gcaggcgatc gaactgctac tcgcgcactc cgcgggctat
     3121 gtcgagctgt tctacggcca gccgcgcagt caatcctcgt gggaactggt caccgacgct
     3181 ctggcgcgca gcaagtccgg gatgctggtc ggtggcgcga agcggctgta cggcattgtc
     3241 gaagggggcg atttggctta cgtggaggag cgcgttgacg ccgacggcgg cctggtgccg
     3301 catctgtcgg cccgcctgac gaggttcgtc ggctgatgcg cagggttcgg ttggccgcgg
     3361 cgctcgccgc ggcggtcatc atcgcggggt gttcgtcgaa ccaaagtccg tccgaggagt
     3421 cgtcgacgca gtcgccggcg cccagtgggc acggttcata tgcgagttgt cttcacgagc
     3481 acggcgtttc ggctccgccc gggccggtcg ccggtcctcc acccggcacc gacgaggcca
     3541 cttggcacaa ggccacgcag gcctgtgcgt cgctggcgcc cggcccggat gccggctagc
     3601 ggggaagtga tccggccctg gatatgggtc ggcccccgag ccgctgttcc tggtcggaca
     3661 agaccgaggg gctcgggggc cgggtgactg cggggaattc gccagcgcgc gcaggtgact
     3721 ccccggcgct gctagctcgc agccacctca catgtccata agtcaatcaa tttctcggac
     3781 cacctccttc cgtgtgtacg gccgaaatta ccactgaccc tgaccgggct gctagttatt
     3841 tctcggtcgg ctgacaagcc tgacggccgc tgcggcacgt cccggttggt gccagggtcg
     3901 acgcgcttcc ccggggtggc gcggcggcgc cgacagccgg tggaggctcg cgctatgtgg
     3961 cgctcagcga tcgctgacga tggcggcgtc gacgagctcg gtgagttccg cggccatcgg
     4021 agaaggcggc agcgccctgc cgtcgagggt gtgcacccga gccgcgaggg tgatgctcga
     4081 taccagccaa accccttgtg cggcgaacag atcaccgggc tgcagggcgc ggtagtcaca
     4141 gtcatagccc ttgttgcgcg ccacctcgaa cagcgcctgc tgggtggtgc cgcgcaagat
     4201 cggataccac ggtggcgggg tcaggagaca aggtcgtccg tcctcctcga tcgcgatgac
     4261 caccgtcgac cgcggccctt caagtacccg gccctccgaa ctgatgaaga tcacatcgcc
     4321 cgcgccctgc cgctcggcat gccg
//
LOCUS       DASYLX010000396         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3419991,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000396 DASYLX010000000
VERSION     DASYLX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419991"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VGP27_09755"
     CDS             <1..510
                     /locus_tag="VGP27_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851633.1"
                     /translation="GKSIRRPLAESLERWHDVERTMALNYYSPLRLIRALAPGMLERG
                     DGHIINVATWGVFSESSPLFAVYNASKAALSAVSRVIETEWSHKGVNSTTLYYPLVAT
                     PMIAPTKAYSGLPALTPSEAADWMVTAARLRPVRIAPRMAVTAKALDTVAPGLLNALM
                     KRQRLQPGA"
     gene            555..944
                     /locus_tag="VGP27_09760"
     CDS             555..944
                     /locus_tag="VGP27_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851634.1"
                     /translation="MEILASRMLLRPADYERSMRFYRDELGLAIAREYDGGTVFYAGQ
                     SLVELAAHGGPNAGGTLWLQVRDVYATEKELAGRGVPIAREARHEPWGLHEMHVTDPD
                     GVTLMFVQVPETHPIRRDTRRWTAPSS"
     gene            complement(969..1280)
                     /locus_tag="VGP27_09765"
     CDS             complement(969..1280)
                     /locus_tag="VGP27_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEV7851635.1"
                     /translation="MTETNQSVLSNVLGWLHQGYPDGVPPKDYFALLALLKRSLTENE
                     VIQAAQAVLKKSAATDTVSENEIRTAIHRVIEKEPNPEEIHQVAARLASVGWPLADCS
                     H"
     gene            complement(1286..1570)
                     /locus_tag="VGP27_09770"
     CDS             complement(1286..1570)
                     /locus_tag="VGP27_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851636.1"
                     /translation="MTYVEAILKVLVVGLLLGAGLPALFATGMVAFSNGAGGEHSDGT
                     VTAPNPILKYLGLVLFAVVALVILVALLWITRNTLIHHFGFDPFPGVKKG"
     gene            complement(1607..2860)
                     /locus_tag="VGP27_09775"
     CDS             complement(1607..2860)
                     /locus_tag="VGP27_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEV7851637.1"
                     /translation="MSAEMIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKT
                     AVLLAGVLNLVGAFLSVEVAVTVTTSVLKVQDSKSGHLLPNITPSMGLTIIFAGLIGG
                     ILWNLLTWLFGIPSSSSHALFGGLIGAGLAALGMAGVNWTGVTSKVLVPAVAAPVIAG
                     LVAACGTWLVYRITRNVMRKRREDGFRWGQIATASLVALSHGTNDAQKTMGVIALALI
                     TTGHLTGDVKRDGLPFWIIFSCALAIGLGTYIGGWRVIRTLGKGLVEIESPQGFAAEA
                     SSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAEVRWAVAGRMAVAWLVTLPAAA
                     LMGALAFWLSHGVESLTGSDLAGDGLIFVILCALSFYIWWRAQKQKIDASNVNADWDD
                     KTNSVVPAVVREAAVEGKDKDAAAV"
     gene            3091..3393
                     /locus_tag="VGP27_09780"
     CDS             3091..3393
                     /locus_tag="VGP27_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEV7851638.1"
                     /translation="MNKFLAKIVAWITSGYPEGVPGPDRVPLFALMKQRLTDDEVKAV
                     VRELLNRGEFDHVDIGVLITQLTDGLPAPEDVERVRARLARKGWPLDDPRDAEETE"
     gene            3414..4538
                     /gene="menE"
                     /locus_tag="VGP27_09785"
     CDS             3414..4538
                     /gene="menE"
                     /locus_tag="VGP27_09785"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEV7851639.1"
                     /translation="MPSGTSALTALPAVEATLAGSGSAMLPVPADDERQTRLLTTSLR
                     AGEEIDDDVAVVVSTSGTTGTPKGAMLTRAALTASADATHQRLGGPGRWLLALPAHHV
                     AGLQVLVRSVIAGLAPVALSARFDAAELPAAVSAMGSGRRYASMVAAQLDKALSDASA
                     RAALAELDAILIGGGPTPARIAEKAAAAGISVVRTYGMSETAGGCVYDGAPLQGVDVR
                     IDLYGRIALGGTTLAKGYRNPSDDDPFREPGWFVTDDVGTLDTSRKLRVLGRTDDAIS
                     TGGLTVFPQLVENALASHPRVADCAVFGVPDDRLGQRVVAAIVAAGGYPPPTLDELRT
                     HLKTMLDPTAAPREVHVVDELPRRGIGKVDRRRLIADFSG"
     gene            4553..>4942
                     /locus_tag="VGP27_09790"
     CDS             4553..>4942
                     /locus_tag="VGP27_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851640.1"
                     /translation="MIRTLAIAASAVAVFAASAPASADPAQQDSTIYAIGKCYDPGQP
                     PRQRPASFAYNCDNTGVMGDMAWSTWGAGGARGTGTDSSVECQPNCAQGATLVNPIVV
                     HAWNPVSSPSAACPQDVKFYSDLTIAYP"
BASE COUNT          868 a         1602 c         1653 g          819 t
ORIGIN      
        1 ggcaagtcca tccgccgccc gttggccgag tcgctggagc gctggcatga cgtcgagcgg
       61 acgatggccc tcaactacta ctcgccgctg cggctgatcc gtgccctggc gcccggcatg
      121 ctcgagcgcg gcgacggcca catcatcaac gtcgcgacct ggggtgtatt cagcgaatcc
      181 tcgccgctgt tcgccgtgta caacgcctcg aaggccgcgc tgtccgcggt cagccgggtc
      241 atcgaaaccg agtggtcgca caagggcgtg aactcgacca cgctgtacta cccgctggtg
      301 gccacgccga tgatcgcgcc gacgaaggcc tacagcgggc tccctgcgct caccccgagc
      361 gaggccgccg actggatggt gaccgccgcg cgcctcaggc cggtgcgcat cgcgccgcga
      421 atggccgtga cggccaaggc cctcgacacc gtcgcccccg gcttgctcaa cgcgctgatg
      481 aagcggcagc gcctgcagcc gggcgcctga cgcgcctgtg ccgaatacgg cacacgacgt
      541 gatagaccag acgcatggag atcctggcga gccggatgct cctgcggccc gccgactatg
      601 aacgctcgat gcggttctac cgcgacgagc ttggcttggc gatcgcccgc gagtacgacg
      661 ggggcacggt cttctatgcg ggccaatcgc tagtcgagct ggcggcccac ggcggaccga
      721 acgcgggcgg aacgctgtgg ctgcaggtcc gcgacgtgta tgccaccgag aaggagctgg
      781 ccggccgtgg cgtgccgatc gcgcgggagg cccgccatga accctggggt ttgcacgaga
      841 tgcacgtaac ggaccccgac ggcgtcacgc tgatgttcgt gcaggtgccg gagacgcatc
      901 cgattcgccg ggacacgaga cgctggacgg cccccagctc ataagccgag ggccgccagt
      961 cagccagttc agtgtgagca gtccgccaat ggccagccga cagaggccag ccgggcggcg
     1021 acctggtgga tctcctccgg gttgggctct ttttcgatga cccggtgtat ggcggtgcgg
     1081 atctcgttct cgctcacggt gtcggtcgcc gccgacttct tcaggaccgc ttgcgcggcc
     1141 tggatgacct cgttctcggt cagtgaccgc ttcaagagag cgagaagtgc gaaatagtcc
     1201 ttcggcggca caccgtcggg atagccctgg tgcagccatc cgagaacgtt gctcaacacg
     1261 ctctggttgg tctcggtcat tcaggtcagc ctttcttgac gccggggaac ggatcgaagc
     1321 cgaagtggtg aatgagggtg ttcctggtga tccagaggag cgcgaccaga atcaccaggg
     1381 cgaccaccgc aaagagcacg aggcccaggt acttcaggat cggattgggt gcggtgacgg
     1441 tgccgtcgga gtgttcgcca ccggccccgt tcgaaaaggc caccatgcca gtggcgaaga
     1501 gggcgggaag gcctgcgccg agcagcaggc ccacgacgag gaccttgagg atggcttcta
     1561 cgtaagtcat cgatgatttc cctttgttag gaggacgggt cgacggtcag accgcagcgg
     1621 cgtccttgtc cttgccttcg acggcggctt cccgcacaac ggctggaacg acggagttgg
     1681 tcttgtcgtc ccagtcggcg ttgacgttgc tggcgtcgat cttctgcttc tgggcgcgcc
     1741 accagatgta gaacgacagc gcgcacaaaa tgacgaagat cagcccgtcg cccgccaggt
     1801 cggagccggt cagcgactcg accccgtgcg acagccagaa cgccagcgca cccatgagtg
     1861 cggcggcggg cagggtgacg agccaggcga cggccatccg gcctgcgacc gcccagcgca
     1921 cttcggcgcc cggcttgccg acaccgctgc ccaggatcga acctgtggcc acgtgggtag
     1981 tcgacagcgc catgcccgcg gcactggagc tcaaaatgat tgcggccgag gatgcctcgg
     2041 cggcgaagcc ctgcggcgac tcgatctcga cgagaccctt gccgagcgtg cggatgacgc
     2101 gccagccgcc gatgtaagtg ccgagaccga tggccagcgc gcagctgaag atgatccaga
     2161 acggcaggcc gtccctcttc acgtcgccgg tgagatgacc ggtggtgatg agcgcgaggg
     2221 cgatgacgcc catggtcttc tgcgcgtcgt tggtgccgtg cgacagcgca acaagcgacg
     2281 ccgtggcgat ctggccccac cggaaaccgt cttcccggcg ctttctcatc acgttgcggg
     2341 tgatccggta gaccagccag gtgccgcagg cggcgaccag gccggcgatg acgggagcgg
     2401 cgaccgccgg aaccaacacc ttggacgtga cgccggtcca gttcaccccg gccatgccga
     2461 gcgcggccag tccggcgccg atcagcccgc cgaagagcgc atgcgacgag cttgacggga
     2521 tgccgaacag ccaggtcaga aggttccaca ggatgccgcc gatcaagccg gcgaagatga
     2581 tcgtgagccc catcgacggg gtgatgttgg gcaacaggtg accgctcttg ctgtcctgca
     2641 ccttcagcac cgaggtggtc acggtgacgg cgacctcgac cgacaggaag gcgccgacga
     2701 ggttgaggac accggccagc agcacagcgg ttttgggctt gagcgcgccg gtggcaatcg
     2761 atgtcgccat cgcgttgccg gtgtcgtgga acccgttggt gaagtcgaat gccagtgctg
     2821 ttgcgatcag cagaaccaag atgatcatct ctgcgctcat gattgccaat tctgatggat
     2881 tctcaatgag tttgaccagc cgcgggccac cctaaagggc ccttgcgacc aggcgatgtg
     2941 gcggacccgg cgaggtgttc attcagtgtt cacccactgt ctcgaggtcg tttgcccgcg
     3001 acggacaccc ggtccacccc atggcggtgc ttccactatc gtgcttccgt aggatcaagg
     3061 ttggttagcc acgccgtggg gagtcaaacg gtgaacaagt tcctcgcaaa gatcgtcgcg
     3121 tggatcacct cgggatatcc ggagggcgtt cccggacccg accgtgtgcc gcttttcgct
     3181 ctgatgaaac agcggttgac cgacgacgaa gtcaaagccg tcgtaaggga actgctgaac
     3241 cgcggcgagt tcgaccatgt cgacatcggc gttttgataa ctcagctcac cgatggacta
     3301 cccgcgccgg aagacgtcga acgggtgcgt gcccgactgg ccaggaaagg ctggccgctc
     3361 gatgacccac gagacgccga ggagacggaa tagccgtcct ccgaacagtt tccgtgccgt
     3421 ctggaacgtc ggcattgaca gcgctgcccg cggtggaggc cacgctcgca ggcagcggtt
     3481 ccgcgatgct gccggttccc gccgacgatg agcgtcaaac acggcttttg acaacgtctt
     3541 tgcgagccgg cgaagagatc gacgatgacg tggctgtcgt cgtgtcgacg tcgggcacga
     3601 ctggcacccc gaagggtgcg atgctgacca gggccgcgct caccgccagc gcggatgcca
     3661 cgcaccagcg cctcggcggc ccgggacgat ggctactggc gttgcctgcc catcacgtcg
     3721 ccggcctaca ggtgttggtg cgcagcgtga ttgcgggcct tgcacccgtc gcactctccg
     3781 ctcgcttcga tgcggccgaa ctgcccgctg cggtttccgc gatgggatcc ggccgacgct
     3841 acgcgtcgat ggtggccgct caattggaca aggcgctgtc tgacgcgtcg gcccgcgcgg
     3901 cgctggcgga gttggatgca atcctgatcg gcggtggccc gacgcccgcc cgtatcgctg
     3961 aaaaggccgc ggcggctgga atttcggtgg tgcggacata cggcatgagc gagacggcag
     4021 gcggctgtgt gtacgacggc gcgcccttgc aaggagtcga tgtccgcatc gacctctacg
     4081 gccggatcgc cctcggcggc accacccttg ccaagggcta tcgcaatccg tccgacgatg
     4141 atccattccg ggagcccggc tggttcgtca ccgatgacgt tggcactctc gacacttcgc
     4201 gaaaattgcg ggttctcggc aggaccgatg acgcgatcag cacaggcgga ttgaccgtgt
     4261 tcccgcagct tgtcgagaac gcgttggcat cgcacccgcg ggtggccgac tgcgcggtct
     4321 tcggtgtgcc cgacgaccgg ctgggtcagc gtgtggtcgc cgccatcgtg gccgccggcg
     4381 gttatccgcc gccgacactg gacgaattgc gcacccacct caagacgatg ctcgatccga
     4441 cggctgcacc ccgcgaagtg cacgtcgtcg atgaactgcc gcggcgaggc atcggcaagg
     4501 tggaccggcg gaggctgatc gccgacttca gcggctgagc tagcatccgg aaatgattcg
     4561 tacactcgcg atcgccgcaa gtgcagtcgc cgtcttcgct gcgtcagcgc ccgcctcagc
     4621 ggatcctgcg cagcaggaca gcaccattta tgcgatcggc aagtgttacg acccgggcca
     4681 gcccccgcgg cagcgccccg cgtcgttcgc gtacaactgc gacaacaccg gcgtcatggg
     4741 ggacatggcg tggagcacat ggggagccgg cggagcacgg ggaacgggta cagacagctc
     4801 cgtcgagtgc caacccaact gcgctcaggg cgcgacgctc gtcaacccca tcgtcgtgca
     4861 tgcgtggaat ccggtgtcgt caccgagcgc cgcgtgcccg caggacgtca agttctacag
     4921 cgacctgacg atcgcctacc cc
//
LOCUS       DASYLX010000397         5712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3528726,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000397 DASYLX010000000
VERSION     DASYLX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528726"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VGP27_09795"
     CDS             <1..763
                     /locus_tag="VGP27_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV7851641.1"
                     /translation="VNEVVNGVLGECGPTPHPDAPALGVVPGGSANVFARALGIDPDP
                     IEATNQLIDLLTEHRRTKSWRRIGLMDCGERWGVFTAGLGVDGAVVAAIEAQRDKGRK
                     VTASRYIRVAVREMLASARREPTLTLHLPDREPVEGVHFAFVSNSSPWTYANTRPVWT
                     NPNTTFETGLGVFATTSMNVWANLRLARQMLSQKAKIRARHLIRDDDLSWVKVTADTP
                     VACQIDGDYLGLRQDVTFRAVPEALGVVAPPVNGL"
     gene            1015..1269
                     /gene="whiB1"
                     /locus_tag="VGP27_09800"
     CDS             1015..1269
                     /gene="whiB1"
                     /locus_tag="VGP27_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator WhiB1"
                     /protein_id="HEV7851642.1"
                     /translation="MDWRHNAVCRDEDPELFFPVGNSGPALAQIADAKLVCNRCPVTT
                     ECLTWALDSGQDAGVWGGMSEDERRALKRRNARTKARTGV"
     gene            complement(1381..2877)
                     /locus_tag="VGP27_09805"
     CDS             complement(1381..2877)
                     /locus_tag="VGP27_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEV7851643.1"
                     /translation="MSTLGDLLAEHTMLPGNAVDHLHAVVGEWQLLADLSFADYLMWV
                     RRDDGALVCVAQCRPNTAPTVLLADAVGTQADAGHLPLVTAAFKSGTIGRESEQRQPG
                     SAADGDISIEAVPVRYNNHVVAVLTHQAAFPARTASPLERAYLDCAVDLLHMLSEGTF
                     PNVGELSVSRSSPRVGDGFIRLDVAGVVVFASPNALSAYHRMGLNAELEGQNLVAVTR
                     PLISDPFEAHELAEHVRESLVGGSSMRMEVDAGGAAVLLRTLPLVVHGEAAGAAMLIR
                     DVTEIKRRDRALLSKDATIREIHHRVKNNLQTVASLLRLQARRTNNPEGREALIESVR
                     RVSSIALVHDALSMSVDEEVNLDDVVDRILPMMNDVAAVDTPIRISRVGDLGVLDADR
                     ATALVMVITELVQNAMEHAFDGATQPGCVTISAERSARWLDVVVHDDGRGLPEGFSLE
                     KADRLGLQIVRTLVSAELDGSLGMHAAPAGGTDVVLRVPIGRRPRLPQ"
     gene            complement(2902..3117)
                     /locus_tag="VGP27_09810"
     CDS             complement(2902..3117)
                     /locus_tag="VGP27_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEV7851644.1"
                     /translation="MAEDVRAEIVASVLEVVVNEGDQIGEGDTLVLLESMKMEIPVLA
                     EVAGTVSKVSVSVGDVIQAGDLIAVIS"
     gene            complement(3363..3665)
                     /gene="rsrA"
                     /locus_tag="VGP27_09815"
     CDS             complement(3363..3665)
                     /gene="rsrA"
                     /locus_tag="VGP27_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEV7851645.1"
                     /translation="MTHRDDEERWSAPVGPVDPEHPECAAVIAEVWTLLDGECTAESR
                     DKLKHHLEECPACLRHYGVEERVKNLIAKKCGGERAPEALRQRLRIQISRTTIFRT"
     gene            complement(3662..4324)
                     /locus_tag="VGP27_09820"
     CDS             complement(3662..4324)
                     /locus_tag="VGP27_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7851646.1"
                     /translation="MTDLGGPAPDSAPAASAPVRAEESDAELTARFERDAIPLLDQLY
                     GGALRMTRNPADAEDLLQETMVKAYAGFRSFREGTNLKAWLYRILTNTYINSYRKKQR
                     QPAEYPTEEITDWQLAANAEHSSTGLRSAEVEALEALPDSEIKAALQALPEEFRMAVY
                     YADVEGFPYKEIAEIMGTPIGTVMSRLHRGRRQLRELLADVAKDRGFIRGQLDTPEEV
                     SS"
     gene            4578..5069
                     /locus_tag="VGP27_09825"
     CDS             4578..5069
                     /locus_tag="VGP27_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV7851647.1"
                     /translation="MARAATPAIKALQAAEIAHEVLRYHHDPQNQSFGDEAVNELAGV
                     DGIDPGQVFKTLIVALPSGLAVAVLPVASQLSLKATAAALGVPKASMADRAAAERSTG
                     YVIGGISPLGQRKALPTVVDGSALRWDRVLCSAGKRGWDVAVAPRDLIRLTRAVTADI
                     RAG"
     gene            5152..>5712
                     /locus_tag="VGP27_09830"
     CDS             5152..>5712
                     /locus_tag="VGP27_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851648.1"
                     /translation="MTLAGKTMFISGASRGIGLAIAKRAAADGANIALIAKTAEPHPK
                     LEGTVYTAAKELEAAGGQALPIVGDIRDGDSVAEAVAEAVEQFGGIDICVNNASAINL
                     GSITEVPLKRFDLMNGIQVRGTYAVSQACVPHMIGRDNPHILTLSPPVRLEPEWLKPT
                     AYMMAKYGMSLCALALAEELRGEGIAS"
BASE COUNT          989 a         1923 c         1866 g          934 t
ORIGIN      
        1 ggtgaacgag gtcgtcaacg gcgtactcgg cgaatgtggc ccgaccccgc accccgacgc
       61 ccccgccctc ggcgtggtgc ccggcgggtc ggccaacgtg ttcgcccgcg cgctcggcat
      121 cgaccccgac ccgatcgagg cgaccaacca gctcatcgac ctgctcaccg agcaccgccg
      181 caccaagtcc tggcggcgca tcgggctgat ggactgcggt gaacgttggg gggtcttcac
      241 cgccggcctc ggcgtcgacg gggccgtcgt ggcggcgatc gaagcccaac gggacaaggg
      301 ccgcaaggtc accgcgtcgc gatacatccg ggtcgccgtg cgcgaaatgc tggccagtgc
      361 ccgcagggaa ccgacgctga ccctgcacct gcccgaccga gagccggtgg aaggcgtgca
      421 cttcgcgttc gtctccaact ccagcccgtg gacatacgcg aacacgaggc cggtgtggac
      481 caaccccaac acgaccttcg aaaccggact cggcgtcttc gcaacgacca gcatgaacgt
      541 gtgggccaac ctgcggttgg ctcggcagat gctgtcgcag aaggccaaga tccgcgccag
      601 acacctcatt cgcgacgacg acctgtcctg ggtgaaggtg accgctgaca cacctgtggc
      661 gtgccagata gacggggatt acctcgggtt gcgtcaagat gtgacgttcc gggcggtccc
      721 ggaggctctc ggcgtggtcg cgccacccgt gaatggcctc tgaagtgcat caacacccgt
      781 acagcgactc atcgagagcg caggtaagtt gagtgtagta caaacggatg aggctattgc
      841 gaacagtggt ccgtagtgag atagcccacg agttaccgca cggtattgac acctgttcag
      901 cgtgtgggag catcgaagta agcaacagtg cagaaacaat ttgttcgcgc gtgttaacag
      961 ccgaaaaata gctcgtgtgc ccccctgcgc acatagtgag gagagttaga caccatggat
     1021 tggcgccaca acgcggtttg tcgcgacgag gatccggaac tgttcttccc cgtgggaaac
     1081 agcggcccag ccctcgcaca gatcgctgac gcgaagctcg tgtgcaaccg gtgcccggtc
     1141 acgaccgaat gcctgacctg ggcattggac tcgggccagg acgctggcgt gtggggcgga
     1201 atgagcgagg acgagcggcg cgcactcaag cggcgcaacg cccgcaccaa ggcccgcacc
     1261 ggcgtctagc cgcacagcgt ttcaacagtt acggccccga cgcaatgtcg gggccgtaac
     1321 tgtgtgcaaa tgagaactgt ccgaattacg caatcagagt caaaatacgc aaacggtcgc
     1381 ttactgcggg agccgaggcc gcctgccgat cggcacccgc agcaccacat cggtgccacc
     1441 cgcgggcgcg gcatgcatgc cgagcgagcc gtccagctcc gctgacacca gcgtgcgcac
     1501 gatctgcaga cccagccggt cggccttctc caggctgaac ccctcgggca ggccgcggcc
     1561 gtcgtcgtgg acgacgacgt ccagccagcg tgccgaccgc tccgcggaaa tggtcacgca
     1621 gccgggctga gtcgcgccgt cgaacgcgtg ctccatggcg ttctgcacca actcggtgat
     1681 caccatgacc agcgcggtgg cgcggtcggc gtcgagcacg ccgaggtcac cgacccggct
     1741 gatgcggatc ggggtgtcaa cggcggcaac gtcgttcatc atcggcagga tccggtccac
     1801 cacgtcgtcg aggttgacct cctcgtccac cgacatcgac agcgcgtcgt gcaccagcgc
     1861 gatcgacgac acccggcgca cggactcgat cagcgcttcg cggccctcgg ggttgttggt
     1921 gcgtcgggcc tgcagccgca gcagcgacgc gacggtctgc aggttgttct tcacccgatg
     1981 gtggatctcg cggatggtgg cgtccttgga cagcagcgcg cggtcgcgac gcttgatttc
     2041 ggtgacgtcg cggatgagca tggccgcgcc cgccgcctcg ccgtgcacga ccagcggcag
     2101 cgtgcggagc aggaccgccg cgccgcccgc gtcgacctcc atccgcatgc tggatccgcc
     2161 gaccagtgac tcgcgcacgt gttcggccag ttcatgcgcc tcgaacggat cggagatcag
     2221 cggcctggtc accgcaacca gattctggcc ctccagttct gcgttcaggc ccatgcggtg
     2281 gtaggcagac agcgcgttgg ggctggcgaa caccacgaca cctgcgacgt ccagccggat
     2341 gaagccgtca ccgacgcggg ggctggaccg cgagaccgac agctcgccga cgttggggaa
     2401 ggtgccctcg gagagcatgt gcagcaggtc gaccgcgcag tcgagatagg cgcgttccag
     2461 tgggctggcg gtgcgggccg gaaacgcggc ctggtgggtg aggacggcga cgacgtggtt
     2521 gttgtaacgg accggcaccg cctcgatcga gatgtcgccg tcggcggccg aacccggttg
     2581 acgttgttcg ctttcgcggc cgatcgtgcc cgacttgaag gccgcggtga caaggggcag
     2641 gtggccggca tcggcctggg tgccgacggc gtcggccagc agcaccgtcg gcgcggtgtt
     2701 gggtctgcac tgcgccacac agaccagcgc gccgtcgtcg cgccgcaccc acatcaggta
     2761 gtccgcgaac gagaggtcgg ccagcagctg ccactcgccg accacggcgt gcaggtggtc
     2821 gacggcattg ccgggcagca tggtgtgctc ggcgagcagg tcaccgaggg tggacatcgg
     2881 acgctacgaa agaagccagc gctagctgat caccgcgatg aggtcgccgg cctggatgac
     2941 gtcgccgacg gacacactga ccttgctgac cgttccggcg acctcggcga gcaccgggat
     3001 ctccatcttc atcgactcca gcagcaccag ggtgtcgccc tcaccgatct gatcgccctc
     3061 gttcaccacg acctcgagca cactggccac gatttcggcg cgaacatcct cggccatttt
     3121 cacccctttc ggctgctgca tgccgtccta tcgaaccaca accgcatggg gcgcagccct
     3181 gtcaggagcc catgagacac tggttcatcc gcgcccgcag gatgcgggcg cacacattcc
     3241 cctggattaa acggaggtag caccatggcc aagcgtggcc gcaagaagcg tgaccgcaag
     3301 cattcgaagg ccaatcacgg caagcgcccg aacgccggtt cgaagtcggt gcgctaggtc
     3361 cgttaggtcc gaaagatcgt ggtgcggctg atttggatgc gcagccgctg acggagcgcc
     3421 tccggtgccc tctccccgcc gcatttcttg gcgatcaggt tcttgacccg ctcctcgacg
     3481 ccatagtggc gaaggcaggc cggacactcc tcgaggtgat gcttgagctt gtcgcggctc
     3541 tcggcggtgc attcgccgtc cagcagcgtc catacctcgg cgatcaccgc cgcgcactcc
     3601 gggtgctcgg ggtcaaccgg gccaaccggc gcgctccagc gctcttcatc gtcacgatgg
     3661 gtcatgacga cacctcctcg ggggtgtcga gctgtccccg aatgaaacca cgatccttgg
     3721 ccacatcggc caataactcg cggagttggc gcctgccgcg gtgcaaccgc gacatcaccg
     3781 tgccgatggg ggtccccatg atctcggcga tttccttgta cgggaagcct tcgacgtcgg
     3841 cgtagtacac cgccatccgg aactcctcgg gcagcgcctg cagggctgcc ttgatctcgc
     3901 tgtccggcaa tgcctccagc gcctccacct ccgctgagcg cagtcccgtc gaggaatgct
     3961 cggcgttggc tgccagctgc cagtcggtga tctcctcggt gggatactcc gcgggctggc
     4021 gttgcttctt gcggtagctg ttgatgtagg tgttggtcag aatgcggtac agccaggcct
     4081 tgaggtttgt gccttcgcgg aacgagcgga agcccgcata ggccttcacc atggtttcct
     4141 gcagcagatc ctccgcgtcg gccgggttgc gcgtcatgcg cagcgcaccg ccgtagagct
     4201 gatcgagcaa cgggatcgca tcgcgctcga atcgcgcggt cagctccgca tcggactctt
     4261 cggctctcac gggcgccgac gctgccggcg cgctgtcggg cgccgggccg cctaagtcgg
     4321 tcatcctggg gaacaccgtc ccttccgtag aggcgccgcc gatcacgtcg agcaactgca
     4381 ctccacgcac tgggccttct atggccaacg ctggcacctg ggtaccccct tcttcgatcc
     4441 taaaggttga ggtcgacatc ttttccttcg caggccgggc ttcggccgtc actaaaccca
     4501 tcaacagccg gtatggcggc ggttgttccc tcagtcaccg ccgtacggcg gattcgcgcc
     4561 ggttctaggc tgagacggtg gcgcgtgcgg cgaccccggc gatcaaggcc ctgcaggccg
     4621 ccgaaatcgc ccatgaggtg ctgcgatacc accacgaccc gcagaaccag tcattcggcg
     4681 acgaggccgt caacgaactc gccggcgtcg acggcatcga tcccggccaa gtgttcaaga
     4741 cgctgatcgt cgcgctgccc agcggtctgg ccgttgccgt gctgccggtg gcctctcagc
     4801 tctcgctcaa ggccaccgcg gcggctctcg gcgtgcccaa ggcctcgatg gccgaccgag
     4861 cggcggccga acgctcgacc ggctacgtga tcggcgggat ttcaccgctc ggccagcgca
     4921 aggcgctgcc gacggtcgtc gacggctcgg cgctgcgatg ggatcgcgtg ctgtgcagcg
     4981 caggcaagcg cggctgggac gtcgccgtgg ctccgcggga cctcatccgg ctcacccgcg
     5041 cggtgaccgc cgatatccgc gcgggttgag cgcgaaacgg aattccagca gaagaagttc
     5101 gagagtgagg ccctgctgga attccgtttc gcgcgaacta gggtgagcgc catgacgctt
     5161 gccggcaaga ccatgttcat ctccggcgcc agccgcggta tcgggctggc gatcgccaag
     5221 cgcgccgccg ccgacggcgc caacatcgcg ctgatcgcaa agaccgccga accgcatccc
     5281 aagctggaag gcacggtgta caccgcagcc aaggagctgg aggcggcggg cgggcaggcg
     5341 ctgcccatcg tcggcgacat ccgcgacggc gactcggtcg ccgaagcggt ggccgaggcc
     5401 gtcgagcagt tcggcggcat cgacatctgc gtgaacaatg cctcggcgat caacctcggt
     5461 tcgatcaccg aggtgccgct gaagcggttc gacctgatga acggcatcca ggtgcgcggc
     5521 acctatgccg tcagccaagc ctgtgtcccg cacatgatcg gccgcgacaa cccgcacatc
     5581 ctcaccctgt caccgccggt gcggctcgag cccgagtggc tcaagcccac cgcttacatg
     5641 atggccaagt acggaatgtc gttgtgcgcc ttggcattgg ccgaggaact gcgcggcgag
     5701 ggcatcgcat cc
//
LOCUS       DASYLX010000398         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2650507,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000398 DASYLX010000000
VERSION     DASYLX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650507"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1520
                     /locus_tag="VGP27_09835"
     CDS             285..1520
                     /locus_tag="VGP27_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7851649.1"
                     /translation="MRVLLSSWGSRGDVEPLMGLAVALRKLDVEARVCAPPDFAELAA
                     RAGVPLIPLGESLRDMLHGKKPLTPADAPRTAANLVAMQFDTVAPAADGCDAVVASGL
                     MPAGLRTIAEVRGIPYVLVALHPWPIPSPHHAPLSRPGKPFPPGADNRVRWQVDAERV
                     QALYGVPLNTHRAALGMPPVDNVRDHVFTERPWLAADPILAPWPGSPDFDVVATGAWI
                     MPDERPLPADLSAFLDAGTPPVYVGFSSVRAPANVARVAIGAIRASGHRVLVSRGWAG
                     LGVIDDGDDCFAVGEVNHQALFKRVAAVVHHGGAGTTTTAAVAGVPQVVVPQIADQPY
                     WAARLADLGVGAAHDGPTPTLESLSAALDTATSPGTRTRAAALAGRIRTDGATVAARR
                     LVEVITTGRVGTEMPASRR"
     gene            complement(1474..1950)
                     /locus_tag="VGP27_09840"
     CDS             complement(1474..1950)
                     /locus_tag="VGP27_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV7851650.1"
                     /translation="MAKTDRATLESQIAADVRAMTAESDEIGRHFASRHDLTANDFRA
                     LLHVMVGETSGSPLTAGELRTRMGMSGAAITYLVERMIASGHFRRESDPSDRRKVILR
                     VADDGVAVARGFFTPLAKHNRRAMAGLPDDDLAAAHRTFAAIIDAMRAFRSQLDQS"
     gene            2142..>2476
                     /locus_tag="VGP27_09845"
     CDS             2142..>2476
                     /locus_tag="VGP27_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851651.1"
                     /translation="MTEAPEIPPLHMRRDSFSPTAELRQIRESEGVREFTNAFGLQVY
                     LVTRHEDVKAVLADHERFSNERPPGFPVQGVRQPSEEEAANARAGNLLGLDPPEHSRL
                     RRMLTPEFT"
BASE COUNT          395 a          845 c          832 g          404 t
ORIGIN      
        1 aagtccgacg tcggcgttcc ggcggctgcg gctggagatg ctggccgccg aacgcgccgc
       61 gttcatcgcc gagcgcgaca gcggacacat cgacgacgag gtgttgcggt cggtgcttca
      121 cggactggat ctcgaggaag cgacgctcaa ccgggcctaa cgcggagccg tatctcttcc
      181 cacttcaaca tatagcgcac agggggtctt gcggcaagac ccccgtcatg ccccagaatc
      241 gctcccctga gccagaactg gcgatacgag aagggggtag cacgatgcgt gtgctgttgt
      301 cgtcatgggg ttcacgcggg gacgtcgaac cgctgatggg actcgcggtc gcgttgcgca
      361 agctcgatgt cgaggcgcgg gtgtgcgcac caccggactt cgcagaactc gcggcacggg
      421 ccggagtgcc gctgattccg ctaggggaat cgctgcgcga catgctgcac ggcaagaagc
      481 cgctgacacc tgcggatgcg ccgcggacgg cggcgaacct ggtggcgatg cagttcgaca
      541 ccgtcgcacc ggcggccgac ggctgcgacg cggtggtggc gtccgggttg atgccggcgg
      601 gcctgcgcac gattgccgag gttcgcggca tcccgtacgt gctcgtggcg ctgcatccgt
      661 ggcccatccc ctcgccgcac cacgcgccgc tgtcacggcc gggcaagccg ttcccaccgg
      721 gggcggacaa ccgggtccgg tggcaggtgg acgccgagcg ggtgcaggcg ctctacggcg
      781 ttccgctcaa cacgcatcgg gcggcgcttg gaatgccgcc tgtcgacaac gtgcgcgacc
      841 acgttttcac cgaacgcccg tggctggcgg ccgacccgat cctcgccccg tggcccggtt
      901 caccggattt cgacgtcgta gcgaccggcg cctggatcat gcccgacgag cggccattgc
      961 ccgccgacct gtctgccttc ctggacgcgg gtacaccccc ggtgtacgtg ggtttcagca
     1021 gcgtgcgcgc ccccgcgaac gtcgcacggg tggccatcgg agcgatccgc gcgagcggtc
     1081 accgggtgct cgtctctcgc ggctgggccg gcctcggtgt gatcgacgac ggggacgact
     1141 gcttcgcggt cggcgaggtg aaccaccagg ctctgttcaa gcgcgtcgcc gccgtcgtgc
     1201 atcacggagg cgcgggcacg acgacgacgg ccgccgtcgc aggcgtacct caggtggtgg
     1261 tgcctcagat cgcggaccag ccgtactggg cggcccggtt ggccgacctg ggcgtcggcg
     1321 ccgcacacga cggaccgacg ccgacgctcg agtcgctctc ggctgccctt gacaccgcga
     1381 cgagccccgg cacccgcacg cgtgcggccg cgctggcggg caggatccgc accgacggcg
     1441 cgacggtggc cgcccgacgg ctcgtcgagg tgatcacgac tggtcgagtt gggaccgaaa
     1501 tgcccgcatc gcgtcgatga tcgcggcgaa ggtccggtgc gcggcggcga gatcgtcgtc
     1561 gggtaacccg gccatcgcgc gccggttgtg cttcgccagc ggcgtgaaaa agcctcgcgc
     1621 gacggccact ccgtcgtcgg cgacccgaag gatgaccttg cggcgatctg agggatcgga
     1681 ctcgcgccga aagtgccccg aggcgatcat ccgctcgacg aggtaggtga tggcagcacc
     1741 cgacataccc atccgggtcc tgagttcacc cgcagtgagc ggcgaacccg acgtctcacc
     1801 caccatgaca tgcagcaggg cgcgaaaatc gttggcggtc aaatcatgtc gactggcgaa
     1861 gtgcctgccg atctcgtcgg actccgctgt catcgcccgc acatcggccg caatctgcga
     1921 ctccagcgtc gctcgatcgg tcttcgccac ccgctcagca tagcgtgact gattcgtttg
     1981 cttaataatt aagtatttga actattgctg aatcggcagc gtggtgcgcg ccccccgacc
     2041 agcgttcgcc cttcaccaaa atcgccttgc gcggctggag aacccgtctc gggtcaatgc
     2101 gctcatcgga acagttcgtt ttccgcgagt agcgtcgaac catgaccgaa gcgcctgaaa
     2161 taccgccgct gcacatgcgc cgcgattcgt tcagtcctac cgcggagctt cggcagatcc
     2221 gggagagcga gggcgttcgc gaattcacca acgccttcgg cctgcaggtg tacctggtca
     2281 ctcgccacga ggacgtcaag gccgtgcttg ccgatcacga acggttctcc aacgagcgtc
     2341 cgcccggctt ccctgtgcag ggcgttcggc agccgtccga ggaggaggcc gctaacgcac
     2401 gcgccggtaa tctcctcggc ctcgacccgc ccgaacattc gcggctgcgt cgcatgctga
     2461 cacccgagtt cacgat
//
LOCUS       DASYLX010000399         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2871232,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000399 DASYLX010000000
VERSION     DASYLX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871232"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1877)
                     /locus_tag="VGP27_09850"
     CDS             complement(<1..>1877)
                     /locus_tag="VGP27_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV7851652.1"
                     /translation="GVVLAGKVIAAAANGLGLGVAVAGRLLMLPSLPTGLSAAVTLID
                     YQPRLRALVEDRLGKDATDTVLAIAAATVYAITQAPSAVAVEFIRHVGQVGEALAAAR
                     AWEVFEPMLAAGAECIDATPAAQRPCPVPPGIVEKHAELSGVVQVVAALSLGLLTRNL
                     NTAATAAVVTAPKPARNAREAFASTLGRGLADRHGVLPLRPSTLRMLDRVDAVAVDPR
                     VLAIESLRVSQIRNVADGNRGAVWQWAQDRLEGGGLDAGWHRAPADVDGARRNGRSRG
                     EVFVRNAHHPLAHSVLAEIRRAGADVVSVDVDDLDDLRSSFDELYAVDDEVDAALARA
                     VRDLQRNGRTVAVLSANAPRALAAADVAIGLMGTSGVMPWHADLMADDLEGIWRILHA
                     LPAARTVSRRGVELATSGTLLGSLLMIPGVRGVGPGPGIAGAGAALVVGNRAARRVLG
                     DKTPAPAPVQDWHAMSVDRVKRELSNINTRAPQDVLRTSTPASAGRAAAAADSVRHGV
                     AEFLTTLRGELADPLTPVLAVGSAASALLGSPVDAVLVGSVLTGNALLAATQELRAER
                     LLRRLLAVQDPPARRLKDGHPENVEAAQLRLGDTIEVWPGEVVPADARLLEATDLEAD
                     ES"
BASE COUNT          304 a          672 c          633 g          268 t
ORIGIN      
        1 gactcatcgg cctcgagatc ggtggcctcg aggaggcgag cgtcggcggg aaccacctcg
       61 cccggccaca cctcgatggt gtcgccgagc cgcagctgcg cggcttccac gttctcgggg
      121 tggccgtctt tcaaccgccg cgcgggtggg tcctgcaccg ccagcagacg ccgtagcagc
      181 cgttcggccc gaagttcctg ggtcgccgca agcagtgcgt tgcccgtcag caccgagccg
      241 acgagcacgg catcgaccgg tgacccgagc aaggcgcttg ccgccgaccc gaccgccagt
      301 accggggtga gtggatcggc gagctcaccg cgcaacgtgg tgaggaactc cgcaacacca
      361 tggcggaccg aatcggccgc cgcggccgcc cgacccgctg acgccggagt cgaggtgcgc
      421 aaaacatctt gtggtgcacg cgtattgata ttcgacagct cgcgcttcac ccggtccacc
      481 gacatggcgt gccagtcctg gacgggtgca ggcgcgggtg tcttgtcccc gagcacgcgg
      541 cgcgccgccc ggttgcccac cacaagagct gcgcccgcgc cggcgatgcc cggcccaggg
      601 ccgacgcccc ggacaccggg aatcatcagc agcgagccga gaagcgttcc actggttgcc
      661 aattcgacgc cccgccgact cactgtgcgg gcggcgggca gagcgtgcag gatgcgccag
      721 atgccctcaa ggtcgtcggc catcaggtcg gcatgccatg gcatgacacc gctggtgccc
      781 atcaagccga tagcgacgtc tgccgcagcc agcgcgcgcg gtgcgttcgc ggacaacacc
      841 gcgacggtcc tgccgttgcg ctgcaggtct cggacagcgc gcgcgagcgc ggcgtctacc
      901 tcgtcgtcaa cggcatacaa ctcgtcgaat gacgaccgca ggtcgtcgag gtcgtcgacg
      961 tcgaccgaaa cgacatccgc accggcccgt cggatctccg ccagcacgct gtgcgcgagg
     1021 gggtgatggg cattgcggac gaacacttca ccacgtgacc gaccgttcct ccgtgcgccg
     1081 tcgacgtcgg ccggggcccg gtgccaaccg gcgtccagtc cgccgccttc cagccggtcc
     1141 tgcgcccatt gccagaccgc gccacggttc ccgtcggcga cgttcctgat ctgactgacc
     1201 cgcagggact cgatggcgag cacccgcgga tccaccgcga ccgcatccac gcggtcgagc
     1261 atgcgcagcg tcgacggccg cagcggcagc acgccatgcc ggtcggccag gccacgtccg
     1321 agcgtgctgg cgaaggcctc gcgtgcattg cgcgcaggct tgggggcggt cacgacggct
     1381 gcggtggccg ccgtgttgag gttgcgggtc agcaagccca acgagagcgc ggcgacaacc
     1441 tgaacgacac cgcttagctc ggcatgtttc tccacaattc ccggcggcac cggacatggc
     1501 cgttgcgcgg ccggcgttgc gtcgatgcat tcggcacccg cggccagcat cggctcgaaa
     1561 acctcccatg cgcgtgcggc ggccagagcc tccccgacct gtccgacgtg ccggatgaat
     1621 tcgacggcca ccgcggaagg ggcctgggtg atcgcgtaca cagtcgcagc ggcgattgcc
     1681 aggacggtgt cggtggcatc cttgccgagc cggtcttcga ccagcgcgcg caatcgcggt
     1741 tgatagtcga tcaacgtgac ggccgcagac agaccggtgg gcaggctggg caacatcagt
     1801 agcctgcccg cgaccgcgac gccaaggccg aggccattgg ctgcagcggc gatcaccttg
     1861 cctgcgagca ctacacc
//
LOCUS       DASYLX010000400         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2980116,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000400 DASYLX010000000
VERSION     DASYLX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980116"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VGP27_09855"
     CDS             <1..107
                     /locus_tag="VGP27_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV7851653.1"
                     /translation="LTQVLDRHYPGDVVDLTWIDKSGQQRTGKATLTS"
     gene            175..1245
                     /locus_tag="VGP27_09860"
     CDS             175..1245
                     /locus_tag="VGP27_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7851654.1"
                     /translation="MPNTPHRVVQWTTGNVGKSSVEAIAKNPNYELIGCYAWSEDKNG
                     VDVGELAGIEPLGVTATNDVDALLALKPDCVVYNPMWIDVDELVRILSAGVNVVTSAS
                     FITGHNLGAGRDRIEQACKAGNSTIFGSGVSPGFADLLAIVAASVCDRVDKVIVAESA
                     DTTLYDSPDTERPAGFGVPIDDPNLGPMAAHGTAVFEEAVRLVAESLGVELDEVVCET
                     EYAQTTEDLEMASWTIKAGHVAGVFASWQGRVGGRTVVDLNVRWRKGQTLEPDWKLDG
                     DGWKITVEGRPTVNMQVGFLPPPDMIENMKSIEDLFVIGHIMTALPPIHAIPAVVAAA
                     PGIATYNDLPLPQARGVVPTNG"
     gene            complement(1242..1523)
                     /locus_tag="VGP27_09865"
     CDS             complement(1242..1523)
                     /locus_tag="VGP27_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851655.1"
                     /translation="MITLKEVVAGTFLTAGLALGAVGVSVGVANAAPAAPAIAGPQYI
                     SQNGPGRGHGHGHDDEYWWDDHGPRNWDPVDACIGLQGPFGYVQGSACI"
     gene            1803..2804
                     /locus_tag="VGP27_09870"
     CDS             1803..2804
                     /locus_tag="VGP27_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851656.1"
                     /translation="MHDQLAGYIAGPVNAEDLVVAPGGEWVLTSGMTGPTAPIGRLYA
                     VSVTNGACNEIFPYRATSALNAQRFVDQPDLNPFKFRPHGIDVTLRPDGRTELYVVNH
                     GGNESVEVFDVDLDAPRPALKWIGGVRLPGSAVGNDVAAVDDGFVVSTAGDPEGRIQV
                     SVESAMAGADTGEVLEWSPKRGWVTLPGTRINTANGVAVSADGAWVYIGGWNSRCIRK
                     VRRGSSDPEPTSVPVDMMVDNLTWAASGRLLAAGTYGTSMEEFLEGHLSTNPRLGIPS
                     RVLAVDPETLATEQLIDYDPDTFGAATTALQVGREIWVGTARDQGLARFRFPTAQRR"
     gene            complement(2847..3488)
                     /locus_tag="VGP27_09875"
     CDS             complement(2847..3488)
                     /locus_tag="VGP27_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851657.1"
                     /translation="MPSTIGKPVAIFAAATALSSFGVLATAASAQAAPSAVCTFARDT
                     LVVNRSDGQMVTPDTQVGGTGMGPKATLFHMQQGPFTEFDKEAGDASGSIHDYHISFT
                     RGAEQYEGEVTTNGGVTGWIQGGGKGNWSSPDGSVNCSSSTNGGLAKVNADVDVYDQP
                     NGDTGKVIGMLTKDTQVNLVEGGGCPPDDWCQITGTNVPTGNGWAWGSFFSKP"
     gene            complement(3933..4619)
                     /locus_tag="VGP27_09880"
     CDS             complement(3933..4619)
                     /locus_tag="VGP27_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851658.1"
                     /translation="MSVTTTTRKLATVAASATAVAAAGLLSMPVAHAASCTVNGDYLR
                     LQQHEGPYTTTTSVNAKGSALGPGVVTVPPSGTNGTYGAASGSVNGRDITVHITWNDN
                     KGTADFTGTIGDDGIARGTSTGTPIPINLWNPGPWDTAPGQLSCTDDQNAATKTATVL
                     KASSVFDKPDGQGTEYRNANGNAIFAPPGKVQLVEPDLCRNNWCHIVPAAGVNGEAWI
                     YIGENYGTYP"
     gene            complement(4969..>5320)
                     /locus_tag="VGP27_09885"
     CDS             complement(4969..>5320)
                     /locus_tag="VGP27_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7851659.1"
                     /translation="QNARDRTFASAYSVRKTPIATVSMPVTWDELREADPDDYTIATV
                     PDLVKERGDAMADLDAVAQSIEPLLEMVKADEERGLGDLPYPPSYPKMPGEPPRVQPS
                     KKVAANWDDDGNPV"
BASE COUNT          884 a         1742 c         1704 g          990 t
ORIGIN      
        1 cgctgaccca ggtgctcgac aggcactacc ccggcgatgt cgtcgacctg acctggatcg
       61 acaagagcgg ccagcagcgc accggcaagg cgacgctgac ttcctgaccg taaacgtcaa
      121 ttttgtggcc gaaaggcacc gctggaacgg tgccgctatc cctatgctga gcgagtgcca
      181 aacacccctc accgggtcgt ccagtggacg acgggaaatg tcggaaagag ctctgtagaa
      241 gcgatcgcca agaaccccaa ctacgaactg atcgggtgct atgcctggtc ggaggacaag
      301 aacggggtcg acgtcggcga gctggccgga atcgaaccgc tcggtgtcac cgccacgaac
      361 gacgtggacg cgctgctggc attgaagccc gactgcgtcg tctacaaccc gatgtggatc
      421 gacgtcgacg agctcgtgcg catcctgtcg gccggggtga acgtcgtgac gtccgcatcg
      481 ttcatcaccg gccacaatct cggcgccggc cgcgaccgca tcgagcaggc ctgcaaagcg
      541 ggcaattcga cgatattcgg atcgggcgtc agtcccggct tcgccgacct gttggccatc
      601 gtcgcggcgt cggtctgcga ccgggtcgac aaggtgatcg tcgccgagtc cgccgatacg
      661 acgctgtacg actcgccgga caccgagcga ccggcgggct tcggcgttcc catcgacgat
      721 cccaacctgg gcccgatggc cgcgcacggc acggccgtat tcgaagaggc ggtacggctg
      781 gtcgccgaat cgctcggagt cgagctggac gaggtggtgt gcgaaaccga gtacgcgcag
      841 accaccgagg acctcgagat ggcgtcgtgg accatcaagg ccggccacgt tgccggtgtg
      901 ttcgccagct ggcagggccg ggtcggcggg aggaccgtcg tggacctcaa tgtgcggtgg
      961 cgcaagggtc agacgctgga gccggactgg aagctcgacg gcgacggctg gaagatcacc
     1021 gtcgagggtc gccccaccgt caacatgcag gtcggcttcc tgccgccgcc ggacatgatc
     1081 gagaacatga agtcgatcga agacctcttc gtcatcggcc acatcatgac ggcgttgccg
     1141 ccgatccacg cgatccccgc cgtcgtcgcc gcggcacccg ggatagcgac ctacaacgat
     1201 cttccactac cccaggcgcg gggcgtcgta ccgacgaacg gctagatgca ggcgctgccc
     1261 tggacgtacc cgaacgggcc ctgcaggccg atgcaggcgt cgaccgggtc ccagttcctc
     1321 gggccgtgat cgtcccacca gtattcgtcg tcgtgtccgt gaccgtgccc acggcccggt
     1381 ccgttctgcg agatgtactg cggtccggcg atggccggcg cggcgggtgc cgcattcgca
     1441 acgcccacac tcacgccgac ggctcccaac gccaacccgg cggtgagaaa agttcctgcg
     1501 acaacttctt tcagagtgat catactaatc ccctcggact atttcgaacg gtgcacggcg
     1561 ccgggtaccg tttggcgcgc cccgtcggcc aaagaggttg ttactgcaca tcttccggcg
     1621 tccgcgtatc actataacga tcgacgcgac agggcgcgac ggcaggccag ggctgacgcg
     1681 cgcgttggtt tgctccggat cgacccgccg agcgtttggc atcgaattcg gccgttgagg
     1741 tctcaaaacg aagtagccga atacgtatgc cgatgccgtg tcactgcgta atcgttacgg
     1801 gtatgcacga tcagctggcc ggttatatcg cggggccggt aaacgccgag gatctcgtgg
     1861 ttgctcccgg cggtgaatgg gtgctgacat cgggtatgac cgggccgacg gcaccgatcg
     1921 ggcggctgta cgcggtgagc gtgacgaacg gggcgtgcaa tgagatcttt ccgtaccgcg
     1981 ccacttccgc cctgaacgcc cagaggttcg ttgaccagcc ggatctcaat cccttcaaat
     2041 tcaggccgca cggcatcgac gtgacactcc ggcccgacgg gcgcacggag ctttacgtgg
     2101 tcaaccacgg tggcaacgag tcggtggagg tgttcgacgt cgacctcgac gcccctcggc
     2161 cggctctgaa atggattggc ggcgttcgac ttccgggcag tgccgtcggc aatgacgtgg
     2221 ccgcggtcga cgacggcttc gttgtcagca ccgccggcga tccggaaggt cgcatccagg
     2281 tgagcgtcga gtcggccatg gcgggagccg atacgggtga ggtgctggag tggtcgccga
     2341 agcggggctg ggtgacgctg ccaggcactc ggatcaacac cgccaacggc gtcgcggtgt
     2401 ccgcggacgg cgcgtgggtg tacatcgggg gctggaattc gcgctgcatc agaaaggttc
     2461 gacgcggcag cagcgacccg gagccgacct ccgtgccggt cgacatgatg gtcgacaacc
     2521 tgacgtgggc ggcgtcgggc cggcttctcg cagcgggcac ctacggcacc agcatggagg
     2581 aattcctcga aggccatctc agcacgaatc cccgcctggg catcccgtcg cgggtgctcg
     2641 ccgtcgaccc cgagacactc gcaaccgaac agctcatcga ttacgatccc gacaccttcg
     2701 gcgcggcgac gaccgcgttg caggtcggcc gtgagatctg ggtgggcacc gcacgcgacc
     2761 aggggttggc ccggttccgg ttcccgaccg cgcaacggcg ttgacgggct gacgacttgc
     2821 gccctggagc gcttccgccc aggcgcctac ggcttgctga agaatgatcc ccacgcccag
     2881 ccgttgccgg tgggcacgtt cgttccagtg atctggcacc agtcgtcggg agggcaacct
     2941 ccgccttcca cgaggttgac ctgggtgtcc ttcgtgagca tgccgatcac cttgccggtg
     3001 tcgccgttgg gctggtcgta gacatcgacg tcggcgttca ccttcgcaag gccgccgttg
     3061 gtgctgctcg aacaattcac cgaaccgtcg ggcgagctcc aattgccttt gccgccgccc
     3121 tgaatccagc ccgtcactcc gccgttcgtc gtgacctcgc cctcgtactg ctcggcccca
     3181 cgggtgaagc tgatgtggta gtcgtggatg gaaccggagg catcaccggc ctccttgtcg
     3241 aattcggtaa acgggccctg ctgcatgtgg aacagagtcg ccttcggccc catacccgtg
     3301 cccccgacct gcgtatcagg cgtcaccatc tggccgtcgc ttcgatttac gaccagggtg
     3361 tcgcgtgcga acgtacagac cgcagacggc gccgcctgcg ccgaggccgc cgtcgcgagc
     3421 actccgaatg agctgagcgc ggtcgctgcg gcgaagatag cgaccggctt tccgatggtt
     3481 gacggcatga cgaatccttt cgttgatgca cttcccagcc ggtcaccgac ccccgacgag
     3541 cccgctgcgc ggtgccttac tgatgcgtga tgctatgccc gtaacgcgcc cgcaaatatc
     3601 gggtgtttcc ctacgatttc gaaggattcc gataggtttc gcccatcaac ggtcctaaac
     3661 cgggttcccg tcctcgtccc agttcgccgc caccttcttg ctgggctgga cgcgcggcgg
     3721 ctcccttacc gcccgcccgc gaccttccag cgagacgttc tgtctttcaa actggccacc
     3781 gacggcagcg ccgcacacgc ccccacgtat gaccagtggc tcgaagacag tgcgacctcg
     3841 gtctgctcgt agtcgacatc gactatctgg acgaagtcat aaatctcctc ggcaggacaa
     3901 ccggcgccta tcggcccagc tcgccgagag ggctacgggt acgtgccgta attttcaccg
     3961 atgtagatcc aggcctcgcc attcaccccg gcggccggga cgatatggca ccagttgttg
     4021 cggcacagat cgggctccac cagctgaacc ttcccaggcg gagcgaagat cgcattgcca
     4081 ttagcgttcc ggtattcagt cccctggcca tctggcttgt cgaagacgct gctggccttg
     4141 agcaccgttg ccgtcttggt cgccgcattc tggtcgtccg tgcagctcag ctgccccggg
     4201 gcagtgtccc agggccccgg gttccacaag ttgatcggta tcggtgttcc ggtcgaggtg
     4261 ccgcgcgcga tgccgtcgtc gcctattgtg cctgtgaagt cggctgttcc cttgttgtcg
     4321 ttccaagtga tgtggacggt gatgtcgcgg ccgttgacgc tcccactcgc cgcaccgtac
     4381 gtaccgtttg tcccgctggg agggacggtg accaccccgg ggccgagcgc tgacccttta
     4441 gcgttgacgc tggtggtggt ggtgtagggc ccctcgtgct gttggagtct gaggtagtcg
     4501 ccgttgaccg tgcacgatgc tgcgtgcgcc actggcatgg ataggagtcc ggctgcggcg
     4561 acagcggttg ctgaggcggc gacggtcgcc aacttgcggg tggtggtggt gactgacatg
     4621 ttcgtgtcct ttcagccctt tatcgtcaaa ggcatgcacg cgatctctgg cgaaagttgg
     4681 cagcgcacct cgcgaaattg ccggttggct accgtctttt ccgcttgccc cgcaggctaa
     4741 ccctgcaggc cacggaaaga tatcgggtgt ttccctaccg ttcgtggcgc gatgcgtcgg
     4801 caatcaacgc acgcaatcaa cgcaccgctc gaactattgg gtgcggtcgc tgtggttgcg
     4861 gctacagcgg ccattcctca gtcgaccgag actcctgcct ggccaaactc gatgtagcca
     4921 ccatctatca ggactccgag gtcgagggtc tggtgcggga cttcctcgct acaccggatt
     4981 gccgtcgtcg tcccagttcg ctgccacctt cttgctgggc tgcacgcggg gcggctcccc
     5041 cggcatcttc gggtagctcg gcggatacgg caggtcgccc agaccgcgct cctcgtcggc
     5101 cttgaccatc tccagcagcg gctcgatcga ctgcgcgacg gcgtccagat ccgccatcgc
     5161 atcgccccgc tccttcacca gatccggcac ggtggcgatg gtgtagtcgt cggggtcggc
     5221 ctcgcgcagt tcatcccatg tcaccggcat cgacaccgtc gcgatcggcg tcttgcgcac
     5281 cgagtacgcc gatgcgaacg tgcgatcgcg cgcgttctgg
//
LOCUS       DASYLX010000401         8121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1774048,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000401 DASYLX010000000
VERSION     DASYLX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774048"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGP27_09890"
     CDS             complement(<1..338)
                     /locus_tag="VGP27_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV7851660.1"
                     /translation="MVTAQTSPKATSGEIAEVVDGPRAPVKYAVEAIGTFFLVFTVGA
                     SVGSASPFAPLAIGAVLMVMIYAGGHLSGGHYNPAVTLAVLVRRRIGLRDAVAYWVVQ
                     FGAGLLASAVV"
     gene            complement(443..757)
                     /locus_tag="VGP27_09895"
     CDS             complement(443..757)
                     /locus_tag="VGP27_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEV7851661.1"
                     /translation="MIFKGVIDGKPYPNHGLTYRQWSQIPPRQIRLDELVTTTTVLQL
                     DRLLSEDSTFYGDLFPHAVRWRGVTYLEDGLHRAVRAALRNRTVLHARVFDMDAPVAR
                     QM"
     gene            909..1940
                     /gene="hrcA"
                     /locus_tag="VGP27_09900"
     CDS             909..1940
                     /gene="hrcA"
                     /locus_tag="VGP27_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV7851662.1"
                     /translation="MSSADDRRFDVLRAIVADFVATHEPIGSKTLVERHNLGVSSATV
                     RNDMAVLEAEGYITQPHTSSGRVPTEKGYREFVDRLEDIKPLSTSERSAILKFLETGV
                     DLDDVLRRAVRLLAQLTRQVAIVQYPTLSTSTVRHLEVVPLTPARLLLVVITDSGRVD
                     QRMVELGDVIDEHQVSQLREMLGQALEGKRLSVASVAVSELAVHMDGSGGLGDAIGRS
                     ATVLLESLVEHTDERLLLGGTANLTRNTADFGGSLRSVLEALEEQVVVLRLLAAQQEA
                     GKVTVRIGHETEAEEMAGTSVVTTTYGSMGTVYGGMGVLGPTRMDYPGTIANVAAVAL
                     YIGEVLGNR"
     gene            1971..3116
                     /gene="dnaJ"
                     /locus_tag="VGP27_09905"
     CDS             1971..3116
                     /gene="dnaJ"
                     /locus_tag="VGP27_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV7851663.1"
                     /translation="MARDYYGLLGVSKGASDSEIKRAYRKLARELHPDVNPDQEAQAH
                     FQEISVAYEVLSDPEKRRVVDLGGDPLESAGSAGNGFGGFGGLGDVFEAFFGGGATSR
                     GPIGRVRPGADSLLRMRLDLEECATGVTKQVTVDTAVLCDLCQGKGTHGSSRPTTCDT
                     CGGQGEIQTVQRSLLGQVMTSRPCPVCGGVGEVILDPCSRCGGDGRVRARREISVKIP
                     AGVGDGMRVRLAAQGEVGPGGGPAGDLYVEVHEKAHDTFLRDGDDLHCTVSVPMVDAA
                     LGTTVTVDAILNGPTEITIPAGTQPGSVTTLRGHGMPHLRSGVRGDLHAHIEVVVPSR
                     LDHDDVELLRKLKELRIRDVAEVKSTQTGGAGGGGLFSRLRETFTGR"
     gene            3124..3870
                     /locus_tag="VGP27_09910"
     CDS             3124..3870
                     /locus_tag="VGP27_09910"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV7851664.1"
                     /translation="MPAALFYVDALPEVGGLAVVDGDEGFHAANVRRMRVGEQLDLSD
                     GAGAMAHCVIEEVSKGRLSARVCDRWTVEPTTPTVTVVQALPKSDRSELAIELATEAG
                     ADAFVAWQSARCVARWDGSRADKGLRRWRAVARAAARQSRRPHIPSVTGVVTTGELVT
                     DVRKAVSAGTTVLALHESATEAVTKVSFAQVDSLMLVIGPEGGIADDEIAALSRAGAK
                     VVRLGPTVLRTSTAAAVALGALGVLTARWG"
     gene            3989..5023
                     /locus_tag="VGP27_09915"
     CDS             3989..5023
                     /locus_tag="VGP27_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV7851665.1"
                     /translation="MTPSETNADSGSPVRSSINVPPDLVVGLLGSADENLRALERILA
                     ADIHARGNALTLSGEPADVALAERVISELIAIAASGQPLNPEVVRHSVAMLTGTEGES
                     PAEVLTLDILSRRGKTIRPKTLNQKRYVDAIDANTIVFAIGPAGTGKTYLAMAKAVRA
                     LQTKQVSRIILTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALHDMLDPELIPKLMS
                     AGVIEVAPLAYMRGRTLNDAFIILDEAQNTTGEQMKMFLTRLGFGAKMVVTGDITQID
                     LPGGANSGLRSAVQILDGVDDIHIAELTSSDVVRHRLVAEIVDAYAKSEDPGSMNRAQ
                     RRASGSRPRR"
     gene            5035..5571
                     /gene="ybeY"
                     /locus_tag="VGP27_09920"
     CDS             5035..5571
                     /gene="ybeY"
                     /locus_tag="VGP27_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV7851666.1"
                     /translation="MSIEVSNESGLDVSEEELVSVARFVIKKMKVHPAAELSMVLLDT
                     AAMADLHMRWMDLPGPTDVMSFPMDELEPGGRPDAPEPGPSMLGDIVLCPQFAAEQAT
                     AAGHTLGQELALLTVHGVLHLLGYDHAEADEEKEMFALQRQLLTEWVADQVEAYHQDR
                     QTEKDRRLLDKSRFFDEP"
     gene            5568..6860
                     /locus_tag="VGP27_09925"
     CDS             5568..6860
                     /locus_tag="VGP27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV7851667.1"
                     /translation="MTGLGSLLGAIALIGFGGLFAAIDAAISTVSIARVEEMVRDERP
                     GAVRLAQVINERPRYINLVVLLRIACEITATVLLVDYLYEHLGLDWGLIAAAAIMVVV
                     SFVVIGVGPRTLGRQNAYSIALGAAVPLQAISTLLTPISRLLVLMGNALTPGRGFRNG
                     PFASEIELREVVDLAQQSGVVADDERRMIQSVFELGDTPAREVMVPRTEMVWIESDKT
                     AGQATSLAVRSGHSRIPVIGENVDDVIGVVYLKDLVERTYYSNNGGRDTNVSAVMRPP
                     VFVPDSKPLDALLREMQRDRNHMALLVDEYGAIAGLVTIEDVLEEIVGEIADEYDTDE
                     VAPVEELGDQRYRVSARLSIEDLGELYDREFDEDLDVDTVGGLLALELGRVPLPGAEV
                     TWDGLQLRAEGGRDHRGRVRIGTVLVSPSAPEQDGDDD"
     gene            6853..7170
                     /locus_tag="VGP27_09930"
     CDS             6853..7170
                     /locus_tag="VGP27_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV7851668.1"
                     /translation="MTELDAEDAKLVTLARGAMGRAETRSGAAVRDRDGRTYAAAPVS
                     LDALTLTALQAAVAAAVSSGATGVEAAVLVGGSADDAGIFAVRELSGDAAVIVTDRSG
                     TPL"
     gene            7238..>8121
                     /gene="era"
                     /locus_tag="VGP27_09935"
     CDS             7238..>8121
                     /gene="era"
                     /locus_tag="VGP27_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV7851669.1"
                     /translation="MSEFRSGFVCFVGRPNTGKSTLTNALVGTKVAITSDRPQTTRHT
                     IRGIVHRDDFQIVLVDTPGLHRPRTLLGQRLNDLVRDTYSEVDVIGLCIPADEPIGPG
                     DRWIHEQIRTVAPKTTLVAVVTKIDKVSKERLAAQLMAVSELVGQDTEIVPVSATTGE
                     QIDVLIGVLVEQLPPGPAFYPEGELTDEPEEVLMAELIREAALEGVRDELPHSLAVVI
                     EEVSPREGRDDLIDVHANLFVERDSQKGIVIGKGGARLREVGTAARTQIEKLLGTKVY
                     LDLRVKIAKNWQRDPKQLG"
BASE COUNT         1399 a         2654 c         2798 g         1270 t
ORIGIN      
        1 actacggcag acgcgagcag gccggcacca aactgcacga cccagtacgc gacagcgtca
       61 cgcagtccga tccgtcgtcg aaccagcacg gccaacgtca cagcggggtt gtaatgccca
      121 ccggataggt gcccgccggc atagatcatg accatcagga ccgcgccgat cgccagcggg
      181 gcgaacggac tggcactgcc gacactcgcg ccgacggtga ataccaagaa gaaggtccca
      241 attgcctcga ctgcgtactt cacgggcgcc cgaggaccgt caacgacctc ggctatttct
      301 ccgctcgtcg ccttgggcga tgtttgcgct gtaaccatgc tttacagaat tgcggtctgg
      361 ggctgcgcag tcgtcaacgc tgacgcccat tgcgccctcc cgccagcgcc agtctgcgca
      421 tacttgccag ctgcaagtac ctctacatct ggcgggcgac cggagcgtcc atgtcgaaca
      481 cccgggcgtg cagcaccgtc cgattgcgca gtgcggcccg caccgcgcga tgcaggccgt
      541 cctcgagata ggtgacgcct cgccagcgca cggcatgcgg aaacaagtcg ccgtagaagg
      601 tcgagtcctc cgacaacagg cggtccaact gcagcaccgt cgtggtggtc accagctcat
      661 cgaggcggat ctgccgcggc gggatctgtg accactgccg atacgtcagc ccgtggttgg
      721 gatacggctt gccatcgata acacctttga agatcacctg cccgaaatcc ttcgacttgt
      781 cccgcgtggc gatggcttca ccatgatcag aggctagccg taaccggccg atccgccagg
      841 cgaaccgtgt gccgaggcgt tgcccctgct ggtcaccgta aaatggatcc ggtccgggag
      901 gtgaacaaat gagtagtgcc gatgatcgtc ggttcgacgt gttgcgcgcc atcgttgccg
      961 acttcgtggc gacgcacgag ccgatcggct cgaagacgct cgtcgagcgg cacaacctcg
     1021 gcgtgtccag cgcgaccgtc cgcaacgaca tggccgtgct ggaggccgag gggtacatca
     1081 cgcagccgca taccagttcg ggtcgggtgc cgacggagaa gggttaccgc gagttcgtcg
     1141 accgcctgga ggacatcaag ccgctgtcga cgtccgagcg atccgccatc ttgaaattcc
     1201 tcgagacggg ggtcgatctc gacgatgtgc tgcggcgcgc ggtgcggctg ctggcgcagc
     1261 tgactcgaca ggtcgccatc gtgcagtacc cgacgttgtc cacgtcgact gtccgccacc
     1321 tcgaggtcgt cccgctgacg cccgcgcgcc tcctgctagt ggtgatcacc gattccggcc
     1381 gagtcgacca gcgcatggtg gagctcggcg acgtcatcga cgaacaccag gtcagccagc
     1441 tgcgcgaaat gctcggccag gcgctggagg gcaagcggct gtcggtggca tcggtggcgg
     1501 tgtcggagct ggccgtccac atggacggca gcggcgggct cggcgacgcg atcggccggt
     1561 cggcgacggt tctgctggaa tcgctcgtcg aacacaccga tgagcggctg ctgctgggcg
     1621 gcaccgctaa cctgacccgc aacaccgccg acttcggcgg ttcgctgcgc tcggtcctcg
     1681 aggcgctcga ggagcaggtc gtggtgctgc ggctgctggc cgcgcagcag gaggccggca
     1741 aggtcaccgt gcgcatcggg cacgagacgg aagccgagga gatggccggc acgtcggtgg
     1801 tcaccacgac ttacggcagt atgggaacgg tctacggcgg catgggtgtg ctcgggccca
     1861 ctcgaatgga ctatccggga acgatcgcta acgtcgctgc ggttgctctc tacataggcg
     1921 aagtcttagg taaccggtaa aaccgcatca gcggtaggga aggtcaggga gtggcacgcg
     1981 attattacgg cctgctcggg gtgagcaaag gcgcgagtga ttcggagatc aaacgcgcct
     2041 atcgcaagct ggcccgtgag cttcatcccg acgtcaaccc agaccaggag gcccaggcac
     2101 atttccagga gatcagcgtc gcctacgagg tgctcagcga tccggagaag cgtcgcgtcg
     2161 tcgacctcgg cggcgacccg ctggagtccg cgggttcggc gggcaacggc ttcggcgggt
     2221 tcggcgggct cggcgacgta ttcgaggcct tcttcggcgg tggcgccacg tcccgtgggc
     2281 cgatcggccg cgtgcggccc ggtgccgact ccctgcttcg gatgcgtctg gacctcgaag
     2341 aatgcgcgac cggcgtcacc aagcaggtga ccgtcgacac ggccgtgctg tgcgatctgt
     2401 gccagggcaa gggcacacac ggcagctcca ggccgacgac ctgcgacacg tgcggcggcc
     2461 agggcgaaat ccagacggtg cagcgctcac tgctcggcca ggtcatgacg tcgcgcccgt
     2521 gcccggtctg cggcggggtc ggcgaagtca tcctcgatcc gtgcagccga tgcggcggcg
     2581 acggccgggt gcgcgcacgg cgcgagatca gcgtcaagat cccggcaggc gtgggggacg
     2641 gcatgcgggt ccggctggcc gcccagggcg aggtcggccc cggcggcggc ccagcgggcg
     2701 acctgtacgt cgaggtccac gagaaggcgc atgacacctt cctgcgcgac ggcgacgacc
     2761 tgcactgcac cgtctcggtg ccgatggtcg acgcggcgct ggggaccacc gtcaccgtcg
     2821 acgcgatcct gaacgggccg accgagatca cgatccccgc aggcacccaa ccgggctcgg
     2881 tgaccacgct tcgcggccac gggatgccgc atctgcgatc cggggtccgg ggcgatctgc
     2941 atgctcacat cgaggttgtc gttccctcgc ggctcgacca tgacgacgtc gagctgctgc
     3001 gcaagctgaa ggaactccgc atccgcgacg ttgccgaggt gaagtccacc cagaccggcg
     3061 gagcaggcgg cggcgggctg ttcagcaggc tgcgcgagac cttcaccggc cgatagccgc
     3121 cgcgtgcctg cggccctctt ctacgtcgac gccctgccgg aggtcggtgg cctggccgtc
     3181 gtcgacggcg acgagggctt ccacgccgcc aacgtgcgtc gaatgagggt cggtgaacaa
     3241 ctcgacctgt cagacggtgc aggcgcgatg gcgcactgcg tgatcgaaga ggtcagcaag
     3301 ggccggttgt cggcccgcgt gtgcgaccgc tggaccgtcg agccgacgac gccgaccgtc
     3361 accgtcgtgc aggcactgcc caaatccgac cgctccgaac tggccatcga gctggccact
     3421 gaggcgggcg ccgacgcctt cgtagcctgg cagtccgccc gctgcgtggc gcgctgggac
     3481 gggagccggg ccgacaaggg cctgcgccgg tggcgtgcgg tcgcccgcgc ggccgcccgt
     3541 cagtcccgca ggccgcacat cccatccgtc accggagtgg tcaccaccgg cgagctggtg
     3601 accgatgtcc gcaaggccgt gtccgcggga acgacggtgc tggcactgca cgagtccgcc
     3661 accgaagcgg tgaccaaagt gtccttcgcg caggtggatt cgctgatgtt ggtgatcggg
     3721 cccgagggtg gcatcgccga cgacgagatc gccgcgctgt cccgtgcggg cgcgaaagtg
     3781 gtgcggctgg ggccgacggt gctcaggacg tcgacggcgg cggccgtcgc gctcggtgca
     3841 ttgggggtgc tgacggcgcg ctggggctga cagctgcggc cgacgtcgtc gcaggttaaa
     3901 cattgggatg ggtcggcagg cggcggtaaa ctgggctcag tagcagacaa acaggcaaga
     3961 accccagaaa gtaggcagaa aaccccacgt gacgcccagc gagacgaacg ctgactccgg
     4021 ctcaccggtc cgcagcagca tcaatgttcc gcccgacctc gtcgtgggcc tgctcggttc
     4081 ggccgacgag aatctgcgcg cactcgaacg catcctggcg gccgacatcc atgcccgagg
     4141 caacgccctc accctctccg gcgagcccgc cgatgtggcc ctggcagagc gggtgatctc
     4201 cgagctgatc gcgatcgctg ccagcggtca gccgctgaac cccgaggtgg tgcggcacag
     4261 cgtcgcgatg ctgaccggca ccgagggcga gtcacccgcg gaggtgctga cgctcgacat
     4321 cctgtcgcgg cgcggcaaaa cgatccggcc gaagacgttg aaccagaagc gatacgtgga
     4381 cgcaatcgat gcgaacacca tcgtcttcgc catcgggccc gcgggcaccg gtaagaccta
     4441 tctggcgatg gccaaggccg tccgcgcatt gcagaccaag caggtgtcac ggatcatcct
     4501 gacccgcccc gcggtggaag ccggtgaacg ccttggcttt ctgcccggca cgctgagcga
     4561 gaagatcgac ccctatctgc gcccgctcta cgacgcgctg catgacatgc tggatcccga
     4621 gctgatcccg aagctgatga gtgccggggt aatcgaggtc gcgccgctgg cgtacatgcg
     4681 tggccggaca ttgaacgatg ccttcatcat cctcgacgag gcgcagaaca ccaccggtga
     4741 gcagatgaaa atgttcctga cccggctggg ttttggcgcg aaaatggttg tcaccggcga
     4801 tattacgcag atcgacctgc cgggcggcgc caactcaggg ctgcgttcgg cggttcagat
     4861 actcgacggg gtcgacgaca tccacattgc cgaactcacc agctccgacg tcgtgcggca
     4921 ccgcctggtc gccgagatcg tggacgcgta cgccaaatcc gaggatcccg ggtcgatgaa
     4981 ccgggcgcag cgccgcgcct cgggcagccg gccgcggcga taagcggggt cgccatgagt
     5041 attgaggttt ccaacgagtc gggcctcgac gtttccgaag aggaattggt cagcgtcgcc
     5101 cgtttcgtca tcaagaagat gaaagtgcat cccgcggccg agctttcgat ggtgctgctc
     5161 gacaccgccg cgatggccga cctccacatg cgttggatgg acctgcccgg tccgaccgac
     5221 gtgatgagct ttccgatgga cgaactcgaa cccggcggcc ggcccgacgc gcccgagccg
     5281 gggccgtcga tgctcggaga catcgtgctg tgcccccagt tcgccgccga gcaggccacc
     5341 gcggccggac atacactcgg ccaggagctt gcgctgctga cagtgcacgg tgtgctgcac
     5401 ctgctcggct acgaccatgc cgaagccgac gaagagaaag agatgttcgc gctgcaacgc
     5461 caattgctca ccgagtgggt ggccgatcag gtcgaggcct atcaccaaga ccggcagacc
     5521 gagaaggatc ggcggctgct ggacaaatcc cgtttcttcg acgaaccgtg accggcttgg
     5581 gttcgctgct tggcgcgatc gcgctgatcg gtttcggtgg gctgttcgcg gccatcgacg
     5641 ccgccatcag cacggtgtcg atcgctcggg tcgaggaaat ggtgcgcgac gagcggcccg
     5701 gtgcggtccg gctggcccag gtgatcaacg agcggccgcg ctacatcaac ctcgttgtgc
     5761 tgctgcgcat cgcctgcgag atcaccgcga cggtgctgct ggtcgactac ctttacgagc
     5821 atctcggcct ggactggggc ctgatcgcgg cggcggcgat catggtcgtc gtcagcttcg
     5881 tggtgatcgg cgtcgggccg agaaccctgg gcaggcagaa cgcctactcc atcgcactcg
     5941 gggcggccgt tcccctgcaa gccatttcga ctctgctgac ccccatcagc cgcctgctcg
     6001 tcctcatggg taatgccctg acgcccggcc gaggcttccg caacgggccg ttcgcgtcgg
     6061 agatcgaact acgcgaggtc gtcgatctcg cccagcagag cggcgtggtg gccgacgatg
     6121 agcgacggat gatccagtcg gtcttcgaac tcggcgacac cccggcccga gaggtgatgg
     6181 tgccgcgcac cgagatggtc tggatcgaaa gcgacaagac agcgggccag gcaacctcgc
     6241 tcgcggtgcg cagcgggcat tcccgcatcc ccgtcatcgg ggagaacgtc gacgacgtca
     6301 tcggcgtcgt gtatctcaag gacctagtcg agcgcaccta ctactcgaac aacggcggac
     6361 gggacaccaa cgtgtcggcg gtgatgcgcc cgccggtctt cgtgcccgac tccaaaccgc
     6421 tggacgcgct gctgcgcgag atgcagcggg accgcaacca catggcactg ctcgtcgacg
     6481 agtacggcgc catcgccggt ctggtcacga tcgaggacgt gctggaggag atcgtcggcg
     6541 agatcgccga cgagtacgac accgacgagg tcgcgcccgt cgaagaactg ggcgaccagc
     6601 gctaccgggt gtcggcccgg ttgtcgatcg aggacctcgg cgagctgtac gaccgcgagt
     6661 tcgatgagga cctcgacgtc gacaccgtcg gcggcctgtt ggccctcgaa ctcggccgtg
     6721 tcccgctgcc tggcgccgag gtgacgtggg atgggctgca gctgcgcgcc gaaggcggcc
     6781 gcgaccatcg cggccgggtg cggatcggca ccgttctggt cagccccagt gcccccgagc
     6841 aggatggtga cgatgactga gctcgacgcg gaagacgcca agctggtgac actcgcccgc
     6901 ggtgcgatgg gccgggccga gacgcgcagc ggagccgccg tgcgggatcg cgacggccgc
     6961 acctacgcag cggcgccggt gagcctcgat gcgctgacgc tgaccgcgct gcaggccgcc
     7021 gtggccgcgg ctgtgtccag tggtgcgacc ggtgtcgagg cggccgtgct ggtcggcgga
     7081 tccgccgacg acgcgggcat ctttgccgtg cgcgagttgt cgggcgacgc cgcggtcatc
     7141 gtcaccgacc gatcggggac gccgttgtga acgccgagac tgcgcacaga tcgcggagtc
     7201 ggccatcttg gcgacctccc cgcaggctcg aggacatatg agcgaattcc gttccggctt
     7261 cgtgtgtttc gtcggcaggc ccaacacggg caagtcgacg ttgacgaacg cgctcgtcgg
     7321 caccaaggtg gcgatcacgt ccgaccggcc gcagaccacg cggcatacca tccgcgggat
     7381 cgtgcaccgc gacgacttcc agatcgtgct cgtcgacacc ccgggcctgc accggccacg
     7441 gactctgctg ggccagcggc tcaacgacct tgttcgggac acctattcgg aggtcgacgt
     7501 catcgggttg tgcatacccg ccgacgagcc gatcgggccg ggggaccggt ggattcacga
     7561 gcagatccgc accgtcgcgc ccaagaccac gctggtcgcg gtcgtcacca aaatagacaa
     7621 ggtgtccaag gaacggttgg ccgcgcagct gatggcggtc agcgagctgg tcggccagga
     7681 caccgagatc gtgccggtgt cggcgaccac cggggagcag atcgatgtgc tgatcggtgt
     7741 gctggtcgaa cagctgccgc cggggcctgc gttctatccc gagggcgagc tgaccgatga
     7801 gcccgaggaa gtgctgatgg cggagctgat ccgcgaggcc gcgctggagg gtgttcgcga
     7861 cgagctgccg cactcgctgg cggtggtgat cgaggaggtc agcccgcgtg agggccgcga
     7921 cgacctgatc gatgtgcacg ccaacctgtt cgtggagcga gacagccaga agggcatcgt
     7981 gatcggcaag ggcggtgcca ggctgcgcga ggtgggcacc gccgcacgca cgcagatcga
     8041 gaagctgctc ggcaccaagg tgtatctgga cctgcgcgtc aagatcgcca agaactggca
     8101 gcgcgatccc aaacagctcg g
//
LOCUS       DASYLX010000402         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3199244,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000402 DASYLX010000000
VERSION     DASYLX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199244"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..140)
                     /locus_tag="VGP27_09940"
                     /pseudo
     CDS             complement(1..140)
                     /locus_tag="VGP27_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(189..>1608)
                     /locus_tag="VGP27_09945"
     CDS             complement(189..>1608)
                     /locus_tag="VGP27_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851670.1"
                     /translation="GAPVLFKDRDICGQCRRREARETEKAKCPRCGKRGTLRDSTGWC
                     GPCSRPGRPPNPDAACVDCGQITRLTGAGRCRRCWDRSPHRPLVRATNLATALHHPPP
                     WLAEFAVYLTPRHHPRHACAILTHLGRLLLDAGPAHPQSLLERATAHNGRLARALEDF
                     FTSTKLALPSGREERHATARRRRRVEAVPQPLRPAAAAFAEHLLAGRERARKSGTRPR
                     GHHTLDARLTAVRDLAVFLVTDRDKTDWATVEVGDIEAFLRNRSSTRASYLAGLRQFF
                     RFAIRSRLVLIDPTHGLTAPQPRGFRGPTLTGDRQRELFHRWTTDPTVHPHEAFVGLL
                     ALLHGATTSEIQHLTDGAIDHSRRKVLLGRRPHPTPLDPWTWTALHNCLAHREALRSN
                     NSHVLITKQSKATRAPASDGYVKNTLRAIGIQPRILRSTRITDLVTTVDAKLVSAAYG
                     MTNEAVTAYLADHVDTIRLSNL"
BASE COUNT          235 a          445 c          648 g          280 t
ORIGIN      
        1 atgcacgttt gcacgatagt gtgtggccag acgttctcca ccacctcggg taaacctttg
       61 aggccatcgc agacgaggaa aaacacgtcg cgcacgccgc ggttcttcag gccgacgagc
      121 acgctcatcc agaacttcgc agaagcgcac atgttcgcgc gaacgtgtgc gaggtagtgc
      181 ctaaacggtc ataggttcga tagtcggatt gtgtcgacgt ggtcggcgag ataggcggtg
      241 acggcctcgt tggtcatgcc gtaggccgcg ctgacgagct tggcatcgac ggtggtgacc
      301 aggtcggtga tgcgtgtgga gcgcaggatg cggggttgga taccgatcgc gcgcagggtg
      361 tttttgacgt agccgtcgga ggccggggcg cgggttgcct tgctttgctt ggtgatcagc
      421 acgtgagagt tgttgctgcg cagtgcctcg cggtgggcta ggcaattgtg cagggcggtc
      481 caggtccagg gatccagtgg tgtggggtgg ggacggcggc cgaggaggac cttgcgacgg
      541 ctgtggtcga tcgcgccgtc ggtcaggtgc tggatctcgc tggtggtggc gccgtggagg
      601 agtgcgagca ggccgacgaa tgcctcgtgg gggtggacag tcgggtcggt ggtccagcgg
      661 tggaacagct ctcgttgccg atcgccggtc agcgttgggc cgcggaaccc ccggggttgg
      721 ggcgcggtca ggccgtgggt cggatcgatc aggacgagcc ggctgcggat ggcgaagcga
      781 aaaaactgtc gcaacccggc caggtaggaa gcgcgtgtgg atgatcggtt gcgaaggaac
      841 gcttcgatgt caccgacctc gacggtggcc cagtcggttt tgtcgcggtc ggtgacgagg
      901 aagacagcca ggtcccggac ggcggtgagc cgggcgtcga gcgtgtggtg cccgcgtggt
      961 cgggtaccgg atttgcgagc acgttcgcgc cctgcgagga ggtgctcggc gaacgctgcc
     1021 gcagcgggcc gcaggggttg tggcacggcc tcgacgcggc gccggcgccg ggcggtggcg
     1081 tggcgttcct cgcgcccgct gggcaacgcc aacttggtgc tggtgaagaa gtcctccagg
     1141 gcgcgggcca gtcggccgtt gtgggctgtg gcgcgttcca ggagcgattg gggatgggcc
     1201 ggacctgcgt cgagcagtaa ccggccgagg tgggtgagga tggcgcaggc gtgccggggg
     1261 tggtggcgag gtgtgagata gacggcgaac tcggccagcc aaggcggcgg atggtgcagt
     1321 gccgtggcca ggttggtggc gcggacgagc ggccggtgcg gggaccggtc ccagcagcgg
     1381 cgacaccgtc cggcgccggt caaccgggtg atctgtccgc agtcgacgca ggccgcgtct
     1441 gggttgggtg gccgtcccgg acgcgagcag ggcccgcacc agccggtcga gtcgcgcagg
     1501 gtgccgcgtt tcccacagcg cgggcacttc gctttctcag tctctcgggc ctcccggcgg
     1561 cggcattgcc cgcagatgtc gcgatctttg aacagcaccg gcgcccca
//
LOCUS       DASYLX010000403         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3529047,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000403 DASYLX010000000
VERSION     DASYLX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3529047"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGP27_09950"
     CDS             complement(<1..653)
                     /locus_tag="VGP27_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7851671.1"
                     /translation="METRDRLFIGNEWVAPATEATIDVISPISEEVYARTPDGSPADI
                     DRAVAAARDAFDNGPWPTMTPQDRADAIAALSQALQKRGDEIAHVVTNENGAPASQSM
                     GMQVFAATMVLDIYADIARSYPWSDERVGAMGQKVVVRRAPVGVAAGIIPWNVPLYIM
                     AMKLGPCLASGSTMVLKPAPETALDPYLLAEAVLEAGLPPGVVNIVTASREASEHIVT
                     "
     gene            961..2739
                     /locus_tag="VGP27_09955"
     CDS             961..2739
                     /locus_tag="VGP27_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851672.1"
                     /translation="MSALTGGVAPALAKPGDHSDPGVPVIPTPTVVVPEAPQVQEQAP
                     VEQPPAPKEAPAPPKVEAPPKQLAPAPEIQAPAPAPEPAPQPQAPAPQIQAPPPQPQA
                     PAPQIQAPAPPKKEAPRVVEPPKADPPKAEAPKVDAPKADAPQMDVPKADTPRVDTPR
                     VDTPKVDAPKVDTPKVDAPKVDDPAPLPAANQPAAPGEPKQDAQIPGNPAPGQTPRGE
                     DNAADGKQPPAVDQVKPGADGARDATGDTKSDPSAVSKAAQRIATAQPDTMQAPKQDL
                     EFARQSKPVELKPEPAKKNDVDFLSSAINVDSKTELGPFESEFRAGSEFSIGDRDRDR
                     NWDRDHDRDWNRDRDHNRDWDWDKKHWDHKVRQWDRKWVEYDDWYRPVFCNPYRAPVR
                     IIYIYERAPRIVIVPPLGRAVIDAAALAAYSFTAVVLNSANLAADVAVGTFFGGGYIP
                     PIGAPFVPPPPLLRYDNVPVQVRYSNATYQPFRVNQIVDLGDDVQYGGRKVLLDGATP
                     AWGQWTTAPTGERQFEVHRTQQYPGLGDPQEAPLPGDYRMQLASDESAADRTQMYLTA
                     AAGVVGGLGLAALGLALYLGRRRLQT"
     gene            2900..>3391
                     /locus_tag="VGP27_09960"
     CDS             2900..>3391
                     /locus_tag="VGP27_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV7851673.1"
                     /translation="MAIRVAHIGTGNAGRIALNHLISDPRFELTGVWVSSDAKVGKDA
                     GELAGLDVSTGIAATNDLDALLATEPDCAVYCAMGDNRIPEAVTDVLRILAAGINVVG
                     TSPGVFQYPWGVMPEKYIAPIEEAARQGNSSLFISGVDPGFANDLIPFAIASTCQSIE
                     QVRC"
BASE COUNT          608 a         1137 c         1127 g          519 t
ORIGIN      
        1 gtgacgatgt gctcgctggc ttcgcgcgac gctgtgacga tgttgacaac gccaggtgga
       61 agcccggcct ccagcaccgc ctcggcgagc aggtacgggt cgagcgccgt ctcgggtgcc
      121 ggcttgagca ccatcgtcga gcccgacgcg aggcacggcc cgagcttcat cgccatgatg
      181 tacagcggca cgttccacgg aatgatcccc gccgcgacgc ccacgggtgc gcggcgcacc
      241 acgaccttct gacccatggc accgacacgc tcgtcgctcc atggatagct gcgcgcgatg
      301 tcggcgtaga tgtcgagaac cattgtggcc gcaaacacct gcatacccat cgactgcgag
      361 gcaggggccc cgttctcgtt ggtgacgacg tgggcgatct catcgccgcg cttctgcagc
      421 gcctgcgaca gcgcggcgat ggcgtcggcc cggtcctgcg gcgtcatcgt gggccacgga
      481 ccgttgtcga atgcgtcgcg ggcagcggcg accgctcgat cgatgtcggc gggcgatccg
      541 tcaggtgtgc gggcgtacac ctcttcagag atcggcgaga tgacatcgat ggtggcttcg
      601 gtggccggcg ccacccattc gttgccgatg aacagtcggt cgcgcgtctc cattgagcac
      661 tccgatcgcg ttgaggtgtg agctgatcgt aaggctgtaa cgcattcctt aggcgagcga
      721 tctaactgtt ggaacggaaa cgtcgccccg ccacaggcgt cggtccaacc aaatccagga
      781 acatcgcggg cgcctgctgg gtccatttcc agtgtcgggg gagatggctg caaagcagtg
      841 gtggcggctg acgccaccct ctgtcagacc agaggagtcg atactcagat gcgtaccccc
      901 cttaaaacgt gctcttggcg aagtgcagtc gctgtgcttt cctccggagc ggtcatttcc
      961 ttgagtgcgc tcaccggggg tgtcgcgccg gccttagcca aacccggcga ccacagcgat
     1021 ccgggcgttc cggtcatccc gacgccgacg gtcgtggtgc cggaggcgcc tcaggttcag
     1081 gagcaggctc ctgtggagca gccgcctgcg ccgaaggagg cgcctgcccc gccgaaggtc
     1141 gaagcgccgc ctaagcagct cgcaccggct cctgagattc aagcgcccgc gcctgcgcct
     1201 gaacccgccc cacaacctca agcgcccgcc ccgcagatcc aggcgccgcc acctcagcct
     1261 caagctcccg caccgcagat ccaggcgccc gccccgccaa agaaagaggc gccgcgagtc
     1321 gtggagccgc cgaaggccga cccgcccaag gcggaggcgc cgaaggtgga tgcgccgaaa
     1381 gccgatgcgc cacaaatgga cgtgccgaag gcagacacac cgagggtcga cacaccgagg
     1441 gtggacacac cgaaggtcga tgcgccgaag gttgacacac cgaaggtcga tgcgccgaag
     1501 gtcgatgacc cggctccatt gcctgccgca aatcagccgg ccgcgccggg cgaaccgaag
     1561 caggatgcgc agatccccgg caatccggcg cccgggcaga cgccccgggg tgaggacaac
     1621 gctgccgacg gcaagcagcc acccgcagtc gatcaggtga agccgggagc cgacggcgca
     1681 cgcgatgcca ccggcgacac gaagagcgac ccgtcggcgg tgtcgaaggc cgcacagcgg
     1741 atcgcgaccg cccagcccga caccatgcag gctccgaagc aggacctcga gttcgccagg
     1801 cagtcgaaac cggtggagct gaagccggaa ccggcgaaga agaacgacgt cgacttcctg
     1861 tcgagtgcga tcaacgtgga cagcaagacc gagctcggcc cgtttgagtc tgaattcaga
     1921 gcgggttcgg aattctcgat cggtgaccgt gatcgcgacc gtaactggga ccgtgaccac
     1981 gaccgtgact ggaaccgtga ccgtgaccac aaccgtgact gggactggga taagaagcat
     2041 tgggaccaca aggtccggca gtgggacagg aaatgggtcg agtacgacga ctggtatcgg
     2101 cccgtcttct gcaaccccta ccgcgcgcct gtgcggatca tctacatcta cgaacgcgcg
     2161 ccccgcatcg tgatcgtccc gccgctcgga cgggccgtca tcgacgctgc cgcgttggcc
     2221 gcatacagct tcaccgcggt ggtgctcaat tcggcgaacc tggccgcgga cgtggccgtc
     2281 ggcacgtttt tcggtggcgg ctacatcccg cccatcggtg cgccgttcgt gccgccccct
     2341 ccgctgttgc gctatgacaa cgtgccggtg caggtgcggt actccaacgc gacctaccag
     2401 ccgttccggg tgaaccagat cgtcgacctc ggcgacgacg tccaatacgg cggacggaag
     2461 gtgctgctcg acggagcgac gccggcgtgg ggccagtgga ccaccgcgcc gacgggcgag
     2521 cgccagttcg aggtgcatcg cacccagcaa taccccggac tcggtgatcc gcaggaggcg
     2581 ccgctgccgg gcgactaccg gatgcagctg gcctccgacg agtccgcggc cgaccgcacg
     2641 cagatgtact tgacggcggc ggcaggagtg gtcggcgggc tgggcctggc ggccctgggc
     2701 ctggccctct acctcgggcg tcgccggctg cagacgtagt ggcgcaggct ggagtgctgg
     2761 acaactgtcc ggcactccag ctcatcccgg gctcgcagcc tcggcgggta ccggcacacg
     2821 ccggtgacct gccttgtttg cctcgggtcg catccgataa gttggacagc tgtccagtcg
     2881 cggccttgag gagatagcaa tggcgatccg cgtcgcacac atcggcaccg gcaacgcggg
     2941 gcgcatcgcg ctcaaccacc tgatcagcga cccccgcttc gaactcaccg gggtatgggt
     3001 gtcgtcggac gccaaggtcg gcaaggacgc gggcgaactc gccggtctcg acgtctcgac
     3061 gggtatcgcg gcgacgaacg atctggacgc actgctggcc acggagccgg actgcgccgt
     3121 ctactgcgcg atgggtgaca accggatacc cgaggcggtg accgacgtgc tgcgcatcct
     3181 ggccgcgggg atcaacgtcg tcggcacgtc tccgggcgtg ttccagtacc cgtggggcgt
     3241 catgcccgag aagtacatcg ctcccatcga agaagctgcg cgacagggca attcgagtct
     3301 gttcatctca ggcgtggatc cggggtttgc caacgatctc atccccttcg ccatcgcgag
     3361 cacgtgtcag agcatcgaac aggtgcgctg c
//
LOCUS       DASYLX010000404         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2323076,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000404 DASYLX010000000
VERSION     DASYLX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323076"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGP27_09965"
     CDS             complement(<1..1104)
                     /locus_tag="VGP27_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7851674.1"
                     /translation="MSSGTDVNIVTKQGRSGRTLPFDMSGVVRTDDGVLRYTTLAPSL
                     LDMLRVSVERHSDRECVVVLGGERVTYRQLWDRSARVAGGLRAAGVQPGDRVANRHGN
                     GLEWCLGFWGTLMAGAVVVPVNTRFSESEVSYVVDDAGAAVVLQPDSPLPDDEPYVRD
                     GAHHQTLAALMYTSGTTGFPKGAMVTHENFLSNVETCVRAMETPATTHIRNLISVPLF
                     HATGCNSQFLPTIRLGGTAVIMPAFDVQAFLRATADEKINVLTSVPAIFWLALNQPDF
                     ADFDMSDVGWVTYGGAPTAPDVIARLRSSFPNARLTNGFGLTETSSVSTLLPDEYAEL
                     RPETVGFAAPPVEVDLIDPDPVTGVGELLIRGQN"
     gene            1163..1711
                     /locus_tag="VGP27_09970"
     CDS             1163..1711
                     /locus_tag="VGP27_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851675.1"
                     /translation="MVTVPAFVWRFGPLVRGVILGVAAGAFLGALAWLDSGFIAIGLI
                     VFVMSCVFYGLWMSRRMVRHWPTAKQLTGPDRERVARAARRGERIDDPALAPALTSYR
                     NGLHEAAQATRIFRWLVWFLLIVAVGMAVWDAAFGSWGNAIVSAIYLVLLAVEVFWWP
                     KRQRRLLANADRAVAMAPEGSA"
     gene            complement(1713..2387)
                     /locus_tag="VGP27_09975"
     CDS             complement(1713..2387)
                     /locus_tag="VGP27_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV7851676.1"
                     /translation="MHSRPSVLVFDVNETLIDIESLAPHFVRIFGDGRVLREWFGQLV
                     MYSMAVTLSDTYVDFFSLGQAVLRMTADIHDVRIGDDDLAALNAGMQTMPAHRDVAEG
                     LSRLKKQGYCLVTLTNSPPVAGAGTPLENAGIAGFFERQFSVDTSRVFKPATGLYTTV
                     ASELGVQPSECMMVAAHVWDTIGAQRAGFSGALIARPGNAPLPAPGVPQPTLVVGDLI
                     ELAERL"
     gene            complement(2380..2814)
                     /locus_tag="VGP27_09980"
     CDS             complement(2380..2814)
                     /locus_tag="VGP27_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851677.1"
                     /translation="MIASLEVVVLPVSDPDKSLDFYRNKLGFALDVDHWPTKDFRVVQ
                     LTPETSRTSIQFGLGLTDAPPGSVQGLYLVVSDITAVRHELVDRGVGVSSIRHKDVDG
                     GQWRGRFRLGADPHRADYSSFADFTDPDGNAWIIQERGHPDA"
BASE COUNT          502 a          911 c         1009 g          529 t
ORIGIN      
        1 gttctggccg cggatgagca gttcgccgac acctgtcacc gggtcgggat cgatgaggtc
       61 gacttcgacg ggcggcgcgg cgaagccgac ggtctccggg cgcaactcgg catactcatc
      121 gggcagcaag gtcgacaccg acgacgtctc ggtaaggccg aatccgttgg tcagccgggc
      181 attagggaac gacgaccgaa ggcgtgcgat gacgtccggt gctgtcggtg ctccgccgta
      241 ggtgacccac ccgacgtcgc tcatgtcgaa atcggcgaag tcaggctggt tcagcgccag
      301 ccagaagatc gcgggcaccg acgtcagcac attgatcttc tcgtcagcgg tcgcccgcaa
      361 gaaggcctgc acatcgaacg ccggcatgat caccgcggtg ccgccgaggc ggatcgtggg
      421 aaggaactgg ctgttgcagc ccgtggcatg gaacagcggc accgagatca ggttgcgaat
      481 gtgggttgtc gcgggagttt ccatggcccg gacgcacgtc tccacgttcg acaggaaatt
      541 ctcatgggtg accatcgcac ccttcggaaa tccggtcgtg ccgctggtgt acatcagggc
      601 ggcaagggtt tggtggtggg cgccgtctct cacgtacggc tcgtcgtcgg gtaatggcga
      661 gtcgggttgg aggacgaccg cggcgcctgc gtcgtcgacg acgtaggaca cctcgctctc
      721 cgagaaacgg gtgttgacgg gcacgaccac cgccccggcc atcagcgtgc cccagaatcc
      781 gaggcaccat tccagtccgt tgccgtgccg gttcgcgacc cgatcccccg gttgcacgcc
      841 tgcggcgcgc aggccacccg cgacacgggc cgagcggtcc cacaactgcc ggtaggtcac
      901 ccgttcgcca ccgagaacaa cgacgcactc gcggtcagaa tgacgctcga ccgacacgcg
      961 gagcatgtcc agcagcgatg gagccaacgt cgtgtagcgc aggacaccgt cgtcggtacg
     1021 gaccacgccg gacatgtcga acggcagcgt tcgcccgctg cggccctgct tggtgacgat
     1081 gttgacgtcc gtgcccgatg acatgtccgt cagtgtggcg tgttggccgt tctaatctgc
     1141 cccaacccga caaaattggt tcatggtgac ggtgcctgcg ttcgtatggc ggtttgggcc
     1201 gttggttcgc ggcgtgatcc tcggtgtcgc cgcgggcgcc ttccttggcg ctctggcgtg
     1261 gctggactcg ggattcatcg cgatcgggct gatcgtcttc gtgatgtcct gtgtgttcta
     1321 cggcctgtgg atgtcgaggc ggatggtgcg gcactggcca accgccaagc agttgaccgg
     1381 gccggaccgc gagcgcgtcg cccgtgccgc ccgacgcggc gagcgcatcg acgacccagc
     1441 actcgccccg gcgctgacca gctaccgcaa cgggctgcac gaggccgcgc aggcgaccag
     1501 gatttttcgg tggctggtgt ggttccttct catcgtcgcg gtcggaatgg cggtgtggga
     1561 cgccgccttc gggtcatggg gcaacgccat cgtgtcggcc atctatctcg tgctgctggc
     1621 ggtcgaggtg ttctggtggc ccaagcgaca gcggcgactg ctggccaacg ccgacagggc
     1681 tgtggcgatg gcacccgagg gatcggcctg agtcagagtc gctcggcaag ctcaataagg
     1741 tcgccgacca cgagcgtggg ctgcgggaca cccggtgcgg gcagcggcgc gttgccggga
     1801 cgggcgatca gagccccgct gaagccggcg cgctgagcac cgatggtgtc ccacacgtgg
     1861 gcggcgacca tcatgcactc cgaaggttgc acccccaatt ccgacgccac tgttgtgtag
     1921 agccccgtcg cgggtttgaa cacccgtgac gtgtcgacgc tgaactgccg ctcgaagaag
     1981 ccggcgatgc ctgcgttctc cagtggtgtg ccggctccgg cgaccggggg agagttcgtc
     2041 agggtaacca ggcagtagcc ctgttttttc aggcgactga ggccttccgc gacatcgcgg
     2101 tgtgcgggca tggtctgcat ccctgcgttc aaagcggcga ggtcatcgtc gccgatccgg
     2161 acgtcgtgga tgtcggccgt catcctcaac actgcttggc cgagagagaa gaagtcgacg
     2221 taggtatccg acagcgtgac ggccatcgaa tacatgacca gttggccgaa ccactcgcga
     2281 agtacccggc cgtcgccgaa gatcctcacg aagtgcggcg ccagggactc gatgtcgatg
     2341 agggtctcgt tgacatcgaa aaccagaacc gacggacggc tatgcatcgg ggtggccacg
     2401 ctcctgaatt atccaggcat tgccgtcggg atctgtgaag tcggcgaagc tcgagtagtc
     2461 ggctcgatga gggtcggcgc cgaggcggaa acgcccccgc cactgcccgc cgtcgacgtc
     2521 cttgtgtctg atcgaactga caccgacccc tcgatcgacg agctcgtgcc gaaccgccgt
     2581 gatatcgctt acgacgaggt agagcccctg gaccgagccg ggtggcgcat cggtcagccc
     2641 aagcccgaat tgaattgatg tgcgcgacgt ttcgggtgtg agctggacga cgcggaagtc
     2701 tttggttggc cagtggtcga cgtcgagggc gaagcctagc ttgttgcggt aaaagtccaa
     2761 cgacttgtcg ggatccgaga cgggcagaac gacgacttcg agcgaagcga tcatcgtgcg
     2821 ctccttgcgg cgtcacggac cagttcgtcg gcgacgagtc cgcggtcgat gccgtgcgcg
     2881 accacggtct gtccgggttc gaggtggagg cgttcgtcgt cgagccacag gtcgatcttg
     2941 tccggctcga a
//
LOCUS       DASYLX010000405         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1334507,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000405 DASYLX010000000
VERSION     DASYLX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334507"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /gene="purB"
                     /locus_tag="VGP27_09985"
     CDS             complement(<1..1185)
                     /gene="purB"
                     /locus_tag="VGP27_09985"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV7851678.1"
                     /translation="MAIPNVLASRYASDEMVAIWSQEAKIVGERRLWLAVLRAQAELG
                     VSVPDGVVADYERVTEDVDLASIAERERVLRHDVKARIEEFNALAGHEHVHKGMTSRD
                     LTENVEQLQIRRSLELVFSHGIAMVARLAERAVSYRDLVMTGRSHNVAAQATTLGKRF
                     ASAAEEMLIALSRVRELIDRYPLRGIKGPMGTAQDMLDLFDGDTSRLADLERRVADFL
                     GFTEVFTSVGQVYPRSLDHDVVSALVQVGAGPSSLAHTIRLMAGHELVTEGFAPGQVG
                     SSAMPHKMNTRSCERVNGLQVVLRGYASMVAELAGAQWNEGDVFCSVVRRVALPDAFF
                     AIDGQTETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILIAAVRAGMGREAAHEIIK
                     ENA"
     gene            1239..2096
                     /locus_tag="VGP27_09990"
     CDS             1239..2096
                     /locus_tag="VGP27_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEV7851679.1"
                     /translation="MHFVGLDLAWGEKNQTGVAAVDADGRLLHVGAAQDDANIIDAIA
                     PFAADECLVAFDAPLIVNNQTGHRPAEAAFNRDFAKFDAGARPAFAEKPELKHPRAAR
                     LAAALDLDMDPASGASRRAIEVYPHPATVVLFELDKILKYKRGPFGDRQRDLLKLMTL
                     IEGLDDATPRLRANRSVAWVELRKRVEAATKPVQLDRDEDPVDAVLCAYVGLYWYHRP
                     DEVTIYGDFDTGYIVTPSLPPDLSPAPRRRVVPPPSDELHERLSRLEDLLAQAQLEAK
                     TIRERLYRM"
BASE COUNT          392 a          778 c          670 g          350 t
ORIGIN      
        1 cgcgttctcc ttgatgatct cgtgcgccgc ctcacgaccc atgcccgcac gcaccgctgc
       61 gatcaggatc cgggtcgtcg ccaggaacgg caggtatcgg tcgagttccc gctggatcac
      121 cgccgggtac gcgccgaact cgtcgagcac cgtcaggaag gtctcggtct gcccgtcgat
      181 ggcgaagaac gcgtcgggaa gcgcgacgcg gcgcaccacc gagcagaaca cgtcgccctc
      241 attccattgc gccccagcga gttcggccac catcgacgca tagccgcgca agaccacctg
      301 cagcccgttc acccgctcgc acgatcgggt gttcatcttg tgcggcatcg ccgacgagcc
      361 gacctgaccg ggcgcgaacc cctcggtaac caactcgtgt cccgccatca accggatggt
      421 gtgcgccagc gacgacggcc ccgcaccgac ctgcaccagc gctgaaacca cgtcgtggtc
      481 aagcgatcgc gggtaaacct ggccgacgct ggtgaagact tcggtgaagc ccaggaaatc
      541 ggcgacgcga cgctcgagat cagcaagccg cgacgtgtcg ccgtcgaaca ggtcgagcat
      601 gtcctgcgcc gtgcccatcg gccccttgat gccgcgcaac ggataccggt cgatcagctc
      661 acgcactcgg gacagtgcga tcagcatttc ctcggccgcc gacgcgaatc gcttgcccag
      721 cgtcgtcgcc tgcgccgcga cattgtggct gcgcccggtc atcaccagat cgcggtagct
      781 caccgcgcgc tcagccagcc gcgcgaccat cgcgatccca tgcgagaaca ccagttccag
      841 agaccgccgg atctgcagct gctcaacgtt ttccgtgagg tcacggctcg tcatgccctt
      901 gtgcacgtgc tcgtggcccg cgagtgcgtt gaactcctcg atgcgggcct tgacgtcatg
      961 gcgcagcacc cgctcgcgct ccgcgatcga ggccaggtcg acgtcctcgg tcacccgctc
     1021 gtagtcggcc accacaccgt cgggaacgga gacgcccaac tcggcctgtg cgcgcagcac
     1081 cgccagccat agtcggcgct cgccgacgat cttggcctcc tgcgaccaga tcgccaccat
     1141 ctcgtcgctg gcgtacctgc tggccagcac gttgggaatc gccacaaata cacagcttac
     1201 ggcggcgggt aggcgcacgt ttgaggcaca gtagccctat gcacttcgtt gggctcgatc
     1261 tcgcttgggg agagaaaaac cagaccggtg tcgcggccgt cgacgccgac ggtcgactcc
     1321 tacacgtggg tgccgcccag gacgacgcca acatcatcga tgccatcgcc ccgttcgccg
     1381 ccgacgaatg cctagtcgcc ttcgatgccc cgctgatcgt caacaaccag accgggcatc
     1441 gacccgccga ggccgcgttc aaccgcgact tcgcaaagtt cgacgcgggg gcgcgcccgg
     1501 cgtttgccga aaagcccgag ctgaagcacc cacgcgccgc ccggttggcg gccgcactcg
     1561 atctcgacat ggacccggct tctggtgcga gccgacgggc gatcgaggtc tatccgcacc
     1621 cggcgaccgt cgtgctgttc gagctcgaca agatactgaa gtacaagcgc ggcccgttcg
     1681 gcgaccgcca acgtgacctg ctgaagttga tgacgctgat cgagggactc gacgacgcga
     1741 caccgcgatt gcgcgcgaac cgcagcgtcg cctgggtcga actgcgcaag cgcgtcgagg
     1801 cggcgacgaa accggtgcag ctcgaccgcg acgaggatcc ggtcgacgcg gtgctctgcg
     1861 cctacgtcgg cctgtactgg taccaccgcc ccgatgaggt gacgatttac ggcgatttcg
     1921 atacgggcta catcgtcacc ccgagccttc ccccggattt gtcccccgcc ccgcgaaggc
     1981 gggtggtgcc gccgccgagt gacgaactac acgaacgcct ttcgcgtctg gaagatctgc
     2041 tggcgcaggc gcaattagaa gcgaagacga tccgcgaacg cctctaccgg atgtgactag
     2101 tcgggtgctt cggccagctg cgtcgggtcg gtgatgcccg cgtcacgcac gccgagctga
     2161 cggttgatca ccctcgtcac aagaaacagc
//
LOCUS       DASYLX010000406         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2323118,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000406 DASYLX010000000
VERSION     DASYLX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323118"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..2644
                     /locus_tag="VGP27_09995"
     CDS             14..2644
                     /locus_tag="VGP27_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEV7851680.1"
                     /translation="MALPTANPHDIDNLLAGYRTARAQQALFDPRGGAVTGYDEFVDA
                     AGNVRPSWLELAECVGERGRGGLEQLRAVVRNLVDNDGITYIQLDTSGEAVVNGDGGA
                     APGPWHLDALPLVISATDWDTLESGLVQRSRLLDAVLADLYGARRCVTGGVFPAQLLF
                     AHPGYIRAARGIEVPGRHQLFMHACDVSRAVGGAFNVNADWTQAPSGAGYALADRRVV
                     AHAVPDLYERIGPRPASPWAQALRLALIDAAPESAEEPVVVVLSPGIHSQTAFDQAYL
                     AGVLGFPLVESADLVVRDGKLWMRSLGTLKRVDVVLRRVDADYADPLDLRADSRLGVV
                     GLVEVLRRGAVTVVNTLGSGILESPGLLRFLPEVAAALLGETPLLDTAPVYWGGINVE
                     RSHLLTKLGSLLIKPATGGATIVGPALTAAQRKDLGVRIEANPWQWVGQELPQFSSAP
                     SDLQPGGLSSARVGMRLFTVAQRGGYAPMIGGLGYLLAPGNAAYRMETVAAKDIWVRT
                     PTRVTAERILTSPPVELPVMTPSPTRAVSSPRVLSDLFWMGRYAERAENTARLLIVTR
                     ERYHEFRYRQDMEGSECVPVLLSALGRITGTDTGETGDSHEMIATAPTTLWSLTADRQ
                     RPGALAQSVERLGLVARAVRDQMSNDTWMVLAAVERAVLHRGGLLPESHTEGEAYLVA
                     AHRGTLAGMLALSGLAAESMVQDVGWTMMDIGKRIERGLALTALLSATLTTVRSLGAE
                     QTVTESALVACESSVIFRRRNLGAVNISAVADLVLLDAENPRSLVYQFERLRADLKAL
                     GSSRPERMVDEIGARLRRIEPDDLEDVGADGRRRELAELLDGLHADLRELSGVITATH
                     LSLPGGMQPLWGPDERRAMP"
     gene            2694..>3154
                     /locus_tag="VGP27_10000"
     CDS             2694..>3154
                     /locus_tag="VGP27_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEV7851681.1"
                     /translation="MSSRRYHVSHRTIYGYSDVVTSSYGRGFLTPRNTDRQRCLSHAL
                     HIDPEPTDSSTSRDAYGNVSSYFHVTERHRELSITSESIVEVDPPPPDECGSGSALAP
                     WEIARPVGPDGALATEFILDLQPPEITDEVRDYAAPSFVPGRPLIEVLRNLT"
BASE COUNT          482 a         1039 c         1118 g          515 t
ORIGIN      
        1 ccgttctgca ctgatggcac tcccgacggc gaatccgcac gacatcgaca acctgctggc
       61 cggctatcgc acggcgcggg cgcagcaggc gctgttcgac ccgcgtggtg gcgccgtcac
      121 cggatacgac gaattcgtcg acgccgcagg caatgtccgg ccgtcctggc tcgagctggc
      181 cgaatgtgtc ggcgaacgcg ggcgcggcgg cctcgagcaa ctgcgcgccg tggtgcgcaa
      241 cctcgtcgac aacgatggca tcacctatat ccagctcgac acgtcgggtg aagccgtcgt
      301 caacggtgac ggcggcgctg cgccggggcc ctggcatctc gacgccctgc cgctggtgat
      361 ctcggcgacc gactgggaca cgctggagtc cggacttgtg cagcgctcgc ggctgctcga
      421 cgcggtgctc gccgacctgt acggcgcgcg ccgctgcgtc accggcggcg tgttccccgc
      481 gcaactgctg ttcgcccatc ccggctacat ccgggccgcg cgggggatcg aggtgccggg
      541 cagacatcag ctgttcatgc atgcctgcga cgtcagccgt gccgtcggcg gcgccttcaa
      601 cgtcaacgcc gactggacgc aggcgccgtc gggcgcggga tacgcgctgg ccgatcgccg
      661 cgtcgtcgca cacgccgtcc cggatctcta cgagcgcatc ggaccccgcc cggcgtcgcc
      721 gtgggcccag gcgttgcggc tggcgttgat cgacgccgcg ccggagtcgg ccgaggagcc
      781 ggtggtggtg gtgctcagcc cgggcatcca ctcccagacc gcgttcgacc aggcgtatct
      841 ggccggcgtg ctcggattcc cgttggtgga gagtgccgac ctggtggtcc gtgacggcaa
      901 gctgtggatg cggtcgctcg gcaccctcaa gcgggtcgac gtggtgctgc ggcgggtgga
      961 cgccgactac gccgatcctc ttgacctgcg ggcggattcg cggctgggcg tggtgggact
     1021 ggtcgaggtg ttgcgcaggg gggcggtgac ggtggtgaac accctcggca gcggaatcct
     1081 ggaaagccct gggctgcttc gttttctgcc ggaagtcgct gcggcgttgc tcggcgagac
     1141 gccgctgctg gacacggcgc ctgtgtattg gggcggcatc aatgtcgagc gttcacacct
     1201 gctgaccaag ctggggtcgc tgttgatcaa gccggccacc ggtggggcca ccatcgtggg
     1261 gcccgcactg acggcggcgc agcgcaagga tctcggtgtc cgcatcgagg ccaatccgtg
     1321 gcagtgggtc ggccaggagc tgccgcagtt ctcttcggcc cccagcgact tgcagccggg
     1381 cggactgtcc tcggccaggg tgggtatgag gctgttcacg gtggcccagc gaggcggcta
     1441 cgcaccgatg atcggcggcc tcggttatct gctggcaccg ggtaacgctg cctataggat
     1501 ggaaaccgta gcggccaagg atatctgggt ccgtacgccg acacgggtca ctgctgagcg
     1561 gattctgacg tcgccacctg tcgagcttcc ggtgatgacg ccaagtccga cccgcgccgt
     1621 cagctcgccg cgtgtgctgt ccgacttgtt ctggatgggg cgctacgccg agcgcgcgga
     1681 gaacaccgcg aggctgttga tcgtcacccg tgagcggtat cacgaattcc ggtaccggca
     1741 ggacatggag ggcagcgagt gtgtgccggt gctgctttcc gcgctgggcc ggatcaccgg
     1801 aaccgacacc ggcgaaaccg gtgactctca cgagatgatc gcgaccgcac cgacgacgct
     1861 gtggtcgctg acggccgacc ggcaacgccc cggcgcgctg gcgcagtcgg tcgagcggct
     1921 cggcctggtg gcccgcgcgg tgcgcgacca gatgtccaac gacacctgga tggtgttggc
     1981 ggcggtggaa cgtgcggtgc tgcaccgtgg cgggttgctg cctgagtcgc ataccgaggg
     2041 tgaggcgtat ctggttgccg cgcaccgcgg aacgctggcc gggatgctgg cgctgtcggg
     2101 actggcagcc gaatccatgg tgcaggatgt cggctggacg atgatggaca tcggcaaacg
     2161 gatcgagcgc ggcctggcac tgacggcact gctcagcgcg acgctgacga cggtccgcag
     2221 cctgggcgcc gaacagaccg tcaccgaatc agcgttggtg gcctgcgaat cgtcggtgat
     2281 cttccgcagg cgcaacctgg gcgcggtgaa catctccgcg gtggcggatc tggtgctgct
     2341 ggacgccgag aacccgcggt cgctggtcta tcagttcgag cggctgcgcg ccgatctcaa
     2401 ggcgctcggc tcatcgcggc ccgagcggat ggtcgacgag atcggcgcca ggctgcgccg
     2461 aatcgaaccg gacgacctcg aggacgtcgg cgccgacggc aggcgacgcg aactcgccga
     2521 actgctcgac gggctgcacg ccgacctgcg tgagctctcc ggtgtgatca ccgcaacgca
     2581 tctgtccctg cccggcggca tgcagccgct gtgggggccc gacgagcgca gggcgatgcc
     2641 gtgagtatcc gaaggcgagc gccgcagcga agcgagagag cggaagccga ggcatgagta
     2701 gtcgccgata tcacgtatct caccgcacga tttacgggta ctccgacgtg gtgaccagct
     2761 cctacggccg tggtttcctc acgccccgca acaccgaccg gcagcgctgc ctctctcatg
     2821 cgctgcacat cgaccccgaa ccgaccgaca gttccacgag ccgggacgcg tatggaaacg
     2881 tcagttcgta cttccacgtg accgaacgcc accgcgaact gtcgatcacc agcgagtcca
     2941 tcgtcgaggt ggacccaccg ccaccggatg aatgtggctc cggatccgcg cttgcgccgt
     3001 gggagatcgc gcgtcccgtc ggccccgacg gcgcgttggc gaccgaattc atcctcgacc
     3061 tgcaaccgcc ggagatcacc gacgaggtgc gggattacgc cgcgccgagt tttgtgccgg
     3121 gccgaccgct gatcgaggtg ctacgaaacc tcac
//
LOCUS       DASYLX010000407         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_347416,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000407 DASYLX010000000
VERSION     DASYLX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347416"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VGP27_10005"
     CDS             complement(<1..406)
                     /locus_tag="VGP27_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TobH protein"
                     /protein_id="HEV7851682.1"
                     /translation="MIAAATIDLDDAEGLLAADRSGLLQGSAMAGAQVRATAAALDEG
                     ALESVAGDPPRTMIWVAGRGNAETAGSILAAALGGSAAAPIVVCAEAPPWIGALDVLV
                     LAGDDSGDPALVSAAATAVRRGARVIVVAPYEG"
     gene            complement(403..1791)
                     /locus_tag="VGP27_10010"
     CDS             complement(403..1791)
                     /locus_tag="VGP27_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV7851683.1"
                     /translation="MSRPAAAVHRVIKAYDVRGLVGEEIDEHFVADVGAAFAGMLRDE
                     GARQVAIGHDMRASSPSLAAAFAEGVVSQGLDVVRIGLASTDQLYFASGLFDCPGAMF
                     TASHNPATYNGIKLCRAGAKPVGKDTGLSTISDDVIAGVPGYHGAAGTIVDREVLAEY
                     GEFLRSLVDLAGLRPLRVAVDAGNGMAGHTAPAVFGAIESLEILPLYFELDGTFPNHE
                     ANPLNPDNLVDLQKHVIATGADIGLAFDGDADRCFVVDESGHPISPSAVTALVAAREL
                     SREIGATVIHNLITSRAVPELVVERGGTPVRSRVGHSYIKALMAETGAIFGGEHSAHY
                     YFRDFWGADSGMLAALHVLAALGEQRRPLSELMADYQRYEASGEINYTVADSQTCVEA
                     VLKSFSDRIESIDHLDGVTVDLGNGNWFNLRTSNTEPLLRLNVEARTLEDVDEVVGRV
                     AAEIAARTEAST"
     gene            complement(1867..2280)
                     /locus_tag="VGP27_10015"
     CDS             complement(1867..2280)
                     /locus_tag="VGP27_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HEV7851684.1"
                     /translation="MNVPRRCCRPGCPHYAVATLTFVYSDSTAVVGPLATVSEPHSWD
                     LCVSHAGRITAPRGWELVRHAGPLPTHPDEDDLIALAEAVREGREAPVGGMVAGFTDP
                     ITGAHGGALMAPPAARHESNGRRRGHLRVLPDPAE"
     gene            2411..2833
                     /locus_tag="VGP27_10020"
     CDS             2411..2833
                     /locus_tag="VGP27_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEV7851685.1"
                     /translation="MRGPLLPPTVPGWRSRAERFDMAVLEAYEPIERRWQDRVSTLDV
                     AVDEIPRMAPKDPESVQWPAEVVADGPIALARLIHAGVDVRGDSTRARIVLFRKPIER
                     RAKDTIELAELLHEVLVAQVATYLGVEPSVIDPSIDDE"
     gene            complement(2843..3109)
                     /locus_tag="VGP27_10025"
     CDS             complement(2843..3109)
                     /locus_tag="VGP27_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7851686.1"
                     /translation="MPERPELDAIAPATLDDDQWQERALCAQTDPEAFFPEKGGSTRE
                     AKRICLGCEVRDACLDYALAHDERFGIWGGLSERERRRLKRGII"
     gene            3547..>3983
                     /locus_tag="VGP27_10030"
     CDS             3547..>3983
                     /locus_tag="VGP27_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEV7851687.1"
                     /translation="MKITVLVGGVGGARFLLGVQHLLGLGQFTGAQTQDHSLAAIVNV
                     GDDAWMYGVRICPDLDTCMYTLGGGIDTERGWGHRNETWHAKEELAAYGVQPDWFGLG
                     DRDLATHLVRSQMLRAGYPLSEVTEALCKRWSPGARLLPASDDR"
BASE COUNT          656 a         1334 c         1338 g          655 t
ORIGIN      
        1 gtccctcgta aggagcgacc acgatgaccc gggcaccacg gcgcaccgcc gtcgccgccg
       61 cgctgaccaa tgccgggtcg ccggagtcgt caccggcgag gaccagcacg tcaagggcgc
      121 cgatccaggg cggcgcctca gcgcagacga cgatcggcgc tgcggccgaa ccacccagcg
      181 ccgcagccag aatcgagccc gccgtctcgg cattgcctcg gccggcgacc cagatcatcg
      241 tgcgcggtgg atcgcccgcg acagactcga gagcaccctc atccagcgcg gcggcggtgg
      301 cgcgcacctg ggcacccgcc atggccgatc cctgcagcag cccgctccgg tcggcggcca
      361 gcagcccctc ggcgtcgtcg aggtcgatcg tggcggccgc aatcatgtcg atgcctctgt
      421 gcgcgcggcg atttcggcgg ccacccggcc gaccacctcg tcgacgtcct cgagggtgcg
      481 agcctcgacg ttgagccgca gcagcggctc ggtgttggag gtccgcaggt tgaaccagtt
      541 gccgttgccg aggtcgacgg tcacgccgtc gaggtggtcg atggactcga tgcggtcgct
      601 gaacgacttc agcacggcct ccacgcatgt ctgcgaatcg gcgaccgtgt agttgatctc
      661 ccctgaggcc tcgtagcgct gatagtcggc catcagttcc gacagcggcc tgcgctgttc
      721 gccgagggcg gcaaggacgt gcagggcggc caacatcccg gagtcagcgc cccagaagtc
      781 gcggaagtag tagtgcgcgg agtgctcgcc gccgaagatg gcgccggttt ccgccatcag
      841 tgccttgatg taggagtgtc cgacgcggga ccgcaccggc gtgccgcccc gctcgacgac
      901 cagttcgggc actgctcgtg aggtgatcag attgtggatc accgttgcgc cgatctccct
      961 gctcaactcg cgtgcggcga ccagggcggt caccgctgac ggcgatatcg gatgcccgga
     1021 ttcatccacc acgaaacacc ggtcggcgtc tccgtcgaag gccagcccga tgtctgcgcc
     1081 ggtggcgatg acgtgcttct gcagatcgac caggttgtcg gggttcagcg ggttggcttc
     1141 gtggttgggg aaggtgccgt cgagttcgaa gtacagcggc aggatctcca gcgactcgat
     1201 cgcgccgaag accgccggcg cggtatggcc cgccattccg tttccggcgt cgacggcgac
     1261 ccgcagtggg cgcagcccgg ccaggtcgac cagcgagcga aggaactcgc cgtactcggc
     1321 cagcacttcg cggtcgacga tcgtgccggc ggcaccgtgg tagccgggca cccccgcgat
     1381 cacgtcgtcg ctgatggtcg agagcccggt gtccttgccg accggtttgg cgcccgcgcg
     1441 gcacagcttg atgccgttgt aggtggccgg attgtggctg gccgtgaaca tcgccccggg
     1501 gcagtcgaac agaccggatg cgaaatacag ctgatcggtc gacgccagcc cgatccgcac
     1561 cacgtcgagt ccctgcgaga ccacaccctc ggcgaacgcg gccgccagcg acggtgaact
     1621 ggcccgcatg tcatggccga tcgcaacttg ccgggcaccc tcgtcgcgca gcatccccgc
     1681 gaacgccgct ccgacgtcgg cgacgaaatg ctcgtcgatc tcctcaccga cgaggccacg
     1741 aacgtcatac gccttgatga cgcggtgcac tgccgcggcg ggtcgggaca tggtttctcg
     1801 gatctcctcg caaagacggg aacaggttgg cgccagccta tccgtcgcgg cgacgaccga
     1861 ttccgattac tccgcagggt cgggcaacac ccgcagatgg ccgcgcctgc gaccgttcga
     1921 ctcgtgccga gccgcaggcg gcgccatcag cgcgccgccg tgagcaccgg taattggatc
     1981 ggtgaaaccg gctaccatgc cgccgaccgg agcctcgcgc ccctcgcgga ccgcctcggc
     2041 cagcgcgata aggtcgtcct cgtcgggatg ggtcggcagc ggcccggcgt gccggaccag
     2101 ctcccagccg cggggggcgg tgatccgccc cgcgtggctc acgcacagat cccaggagtg
     2161 cggctccgag accgtggcca gcggtcccac taccgccgtc gaatcggagt agacgaatgt
     2221 cagcgtcgcg accgcatagt gggggcaccc gggccggcag cagcgacggg gaacattcac
     2281 gaaggtgagg ctatcgcggg ttaaacccgc cgccccgtcg gacacgcagc cgtcggtggc
     2341 ccgacttcca accgttacag ttctccatgt ggccgagcgg aatctccggc gatcacgccg
     2401 aggccgcgac atgcgcggac cgttacttcc tcccaccgtc ccgggttggc gcagccgagc
     2461 cgagcggttc gacatggcgg tgctcgaggc gtacgagccg atcgagcggc ggtggcagga
     2521 ccgggtgagc acgctggatg tcgccgtcga cgagatcccg cggatggctc cgaaggaccc
     2581 ggagagtgtg cagtggccgg cggaggtggt ggccgacggt cctatcgcgc tggcccggct
     2641 gattcatgca ggcgtcgatg ttcggggtga ttccacccgc gcgcgaattg tgttgttccg
     2701 caagccgatc gagcgacggg cgaaggacac catcgagctc gccgagctgt tgcatgaggt
     2761 tctggtggcc caggtggcga cgtatctggg cgtcgaacct tctgtcatcg acccttcgat
     2821 cgacgacgag tgaagccaga ggtcagatta tgccgcgctt gaggcgacga cgctcccgtt
     2881 cggaaaggcc gccccagatg ccgaagcgct cgtcgtgggc cagcgcgtag tccaggcacg
     2941 cgtcgcgcac ctcgcagccc aggcagatgc gcttggcctc ccgtgtcgag ccgcccttct
     3001 cagggaagaa cgcctcgggg tcggtctgcg cgcagagcgc acgctcctgc cattgatcgt
     3061 cgtcgagcgt tgccggcgca atggcgtcga gctccggacg ttccggcaca agactcaatt
     3121 ggggacgccc gacggacgcc atcggtgccg ggccggtatt ggtgtgcggg acgttgccca
     3181 cggagccaag taaccggctg tcgaacctaa tgtggtggcc gaaatcgacc tgctcaaaag
     3241 acatcttccc cctcctcacc ttggtatcgc tgaaccgctg tggagcccca cgatggagat
     3301 gtgcggccat ctcaattcga acaagtgatc gaatctcggt ctgcgacacc ggaaccggcc
     3361 ggccaaccga aaaatgacac tggtgtgatt acacacttgt tagctgctgg ggtcaagctc
     3421 tagaaccgat tttcatacca tctcgttatc tcaattcggc gcgtcgctgc tccggcgtgt
     3481 ctgtcacagc ctcataccgc tggctgccga gacctctccg tgaccgaagg cctagtgtcg
     3541 atcggtgtga agatcaccgt tctggtcggt ggagtcggcg gtgcgcgatt ccttttgggc
     3601 gtgcagcact tgctcggcct gggccagttc acgggcgcac agacacaaga tcattcgctt
     3661 gcggcgatcg tcaatgtcgg cgacgacgcg tggatgtacg gcgtgcggat ctgtccggac
     3721 ctcgacacct gcatgtacac cctgggcggc ggcatcgata ccgagcgcgg ctggggccat
     3781 cgcaatgaaa cctggcatgc caaggaggaa ctcgccgcct acggagtgca gccggattgg
     3841 ttcgggctcg gtgaccgcga tctggccacg cacctcgtcc gcagccagat gctgcgggcc
     3901 ggctacccgc tgtcggaggt caccgaggcg ttgtgcaagc gctggtctcc tggcgcgcga
     3961 ctgctacccg ccagcgacga ccg
//
LOCUS       DASYLX010000408         7996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2323199,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000408 DASYLX010000000
VERSION     DASYLX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323199"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VGP27_10035"
     CDS             complement(<1..141)
                     /locus_tag="VGP27_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family mycofactocin-dependent
                     oxidoreductase"
                     /protein_id="HEV7851688.1"
                     /translation="MAGRLEGKVAFITGGARGQGRAHALAMAKEGADIIVVDICRNID
                     SNP"
     gene            complement(248..1570)
                     /locus_tag="VGP27_10040"
     CDS             complement(248..1570)
                     /locus_tag="VGP27_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851689.1"
                     /translation="MSTRRRLAAGICVLSVALLAAGFGSAVAIADPETGDSVSEHGTE
                     GQQAGQTATGSTGSTAGTGGVGTIPKVVGTVVDSAPPLSSGHVGTNEESPGPTGPMAL
                     GGTTDNQSSGAAAVDDSNKSSDTVQTSATTEETNKPEAVISNSPVTEPSQESQASAAA
                     ADPPATAPANDPPVEEPAPVQPVANDVSLVGDAAPSVQTEPPPPVVGAAQANSVITAL
                     AYFFITLADDGIQLIKLPGEFLSLLGIPLTGDVATASPTVGGIGGSLFAGGLQPATRI
                     DLASSWAVQAGWAGMLIAPGDSSSLSSAGVFTHLTPGGLAASGAAQEHSGLSAVLADG
                     IAPEQVRSLLQHVVDAVLAPLSLLALAALASPGVVGLVLLGVVGMGIGYRQARAASML
                     RAVSIARFVKAGPLGVVRSGGLVAVHPRAARAERRQPRRTADFLSPVA"
     gene            complement(2189..2338)
                     /locus_tag="VGP27_10045"
     CDS             complement(2189..2338)
                     /locus_tag="VGP27_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851690.1"
                     /translation="MSLPSPVTAFRALAELEKRYPSLGCARDETATSPIGQSGIWEAP
                     TDAAW"
     gene            complement(2380..2871)
                     /locus_tag="VGP27_10050"
     CDS             complement(2380..2871)
                     /locus_tag="VGP27_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851691.1"
                     /translation="MKARSTGIAALDERIPFLLSQLGSHVAGEFQRRMAGVGVRPRTY
                     AVLMALATEDGQSQRQLSVGLGIHRNAMVTVIDALEADGLVTRMAHPDDRRAFAVTLT
                     DRARSLLPDLDKASRALEDTITAPLSAIERNTLRELLQRIAGGADLIPGIHPDLADGT
                     ANR"
     gene            2947..3660
                     /locus_tag="VGP27_10055"
     CDS             2947..3660
                     /locus_tag="VGP27_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7851692.1"
                     /translation="MRLAIFGANGQTGRLATTLALDAGHHVVAVTRRPREFPLTAPGL
                     TVTGADVRDASAVGTVVAEVDGVVSALGVTFTREQVDTYSVGTGNVVAAMHAAGTRRL
                     VVVSSTAAYPTRRSHVPFMLRFVEPIIGNTIGKTVYDDIRRMESVVRDSGLDWTIVRP
                     SGLFDLPEPTNYVRGEVDPVGAFTARIDLADYLVSLASAATGARRTVIASTTESTPTL
                     WQMIRREAGSRDSERRAVS"
     gene            3657..4241
                     /locus_tag="VGP27_10060"
     CDS             3657..4241
                     /locus_tag="VGP27_10060"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEV7851693.1"
                     /translation="MTRQKTTAHTHTPIADPADPPGRHGLLQRASAFAAVLFSGLFAG
                     FLITVLVLEESLRSSGAAVYTQVRLVELDHLDDLATALLPPATLAAATLTLALIRSRG
                     SARWLTLAAVILLLATAAISVSINVPINTDQLNWPVLSPPHDWSNVRDRWQLAHATRT
                     TAATLAFVLLTAAVVPLRLPRTAVELSGENQQTG"
     gene            4412..5296
                     /locus_tag="VGP27_10065"
     CDS             4412..5296
                     /locus_tag="VGP27_10065"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7851694.1"
                     /translation="MILVTGATGTIGRPLVELLTAQGAEVRGLARHTGDDLDALPGAV
                     EMVAGDLSRPETIVAALDGVSSLFVHPRAVGDAAPALLALAAERGVEHVAVLSAINVD
                     DDLSWQPSRLNGDRNKEVEDAVIGSGMAWVSVRPCSFAASTSSMWATQIRNGDVVRGP
                     HAEFADPFIHEADVAAVLAAALLDHRLAGQKISITGPQSITHTQMVTTIGAVIGRPLR
                     YEEISPTQAADDIVALGLPRSFAEAMMARYSRDLDRPTPVTVGVKKTLSRPARTYAEW
                     VFDHATAFRNAPAATQTS"
     gene            complement(5364..6188)
                     /locus_tag="VGP27_10070"
     CDS             complement(5364..6188)
                     /locus_tag="VGP27_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7851695.1"
                     /translation="MNLGAWGFAIETEAGDAHLVAAAEIESLGFTALWMAGGQLDRLE
                     QLADLLRATDHVIVAPSIISPDVYDAREVLDLYQSMETTRPGRLLVGLGAPQVPHAIS
                     ALHSYLDELTAAAHPVPLERRMLAAIGPRKLDIARDQFAGAIPILVKPAYTATARERL
                     GPNSTLAVGQFVVLDENPSSARETARTPLRFLLHEVRGYANSARRQGFTDDDISVLSD
                     RLVDALVAWGSPLDIVARAREQRAAGADHVQFTVLHSTGQPGPLEAARRLAPLLNQ"
     gene            6398..7072
                     /locus_tag="VGP27_10075"
     CDS             6398..7072
                     /locus_tag="VGP27_10075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851696.1"
                     /translation="MLDWDSVYKQESDVIQGPAPWNIGEPQPALAELIRQGRFRGDVL
                     DAGWGFAEVSLCLAAEAYTVVGIDIKPTAVAAATAIAAERGLSTATFLHADATSFTGY
                     DGRFSTVVDSTLFHSLPVELRDDYLKAVYRAAAPGASYFILAFAKGAFPAELAAKPNE
                     VTDDELRAAVAKYWIVDDIRPASIYTMLPQYSSAARPVSFPPFEIDEKGRPKLAAWLL
                     TAHKPG"
     gene            7165..7803
                     /locus_tag="VGP27_10080"
     CDS             7165..7803
                     /locus_tag="VGP27_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7851697.1"
                     /translation="MKITVFGATGGIGRQLVAQALNHGHDVTAVVRDAARLPISDVAL
                     HVVTVPGLEQPDLLRSALIGSHAVLSAVGPRGRKDGPVASTAVRTILNAMDSAGVRRL
                     VAVSAAPVGPVPDGESALNRWLLLPLVGRLLRSVYTDLGEMEAQLLRSSAAWTVIRPP
                     KLVNKPLTGIYRTTLGGNVPRGISISRADAAHLMLAALDQPATVTQAVGVAY"
BASE COUNT         1427 a         2674 c         2534 g         1361 t
ORIGIN      
        1 cgggttcgag tcgatgttcc ggcagatgtc gacgacgatg atgtcggcac cctccttggc
       61 catcgcaagc gcgtgcgcac gtccctgacc gcgggctcca cccgtgatga aagcgacctt
      121 gccctcgagc cgtccagcca tatgtcctcc gcgtgaatga agttctccaa caaggcgaac
      181 ctaatttacg cagcctgttt aatcaatgcc cccggaacgg atgtttggtt ttgcggggcc
      241 gtcccgctca ggcgacagga gagaggaaat ccgccgttcg gcgtggctgt cgccgctcgg
      301 cgcgtgcggc gcgcggatgt acggcgacga ggccaccaga cctgacgacc cccagtggtc
      361 ccgccttcac gaagcgggcg atactcaccg cccgcagcat cgacgcggcc ctggcttggc
      421 gatacccgat gcccatcccc acgactccga gaagcacgag tccaacgact cccggcgaag
      481 ccagtgccgc cagcgccagc aaggacaagg gggcgaggac ggcgtcgacg acgtgttgaa
      541 gaagcgaccg cacctgctcg ggtgcgatcc cgtccgccag caccgcgctc agcccggagt
      601 gttcttgagc ggccccactg gccgcgaggc ccccgggagt cagatgtgtg aaaacaccag
      661 cggatgacag cgaactcgaa tcgccagggg cgatgagcat cccggcccag cctgcctgca
      721 ccgcccacga cgaagccaaa tcaatacgcg tggccggttg aaggccaccg gcaaagaggc
      781 ttccgccgat tcccccgacg gtcggcgaag cggtcgccac atcacccgtc agaggtatgc
      841 cgagcaatga gagaaattcg cccggcagtt tgatgagttg gatcccgtca tctgccagtg
      901 tgatgaagaa gtacgccagg gcggtgatga cgctgttcgc ctgcgcggcg ccgacaaccg
      961 gcggcggcgg ttcggtctgg accgacggcg ccgcatcgcc gaccagtgaa acgtcattcg
     1021 cgaccggttg aaccggcgca ggctcctcga ccggtggatc gttggccggc gcggtcgcgg
     1081 gcgggtccgc agcggcggcc gatgcctgcg actcctgcga gggctccgtc accggcgaat
     1141 tcgagatgac ggcctccggc ttgttggttt cctcggtggt cgccgacgtc tggacggtat
     1201 cggaagactt attgctgtcg tcgactgccg ccgctccgct cgactggtta tcggtcgtcc
     1261 cgcccaaggc catgggcccg gtcggaccgg gggattcctc gttggttccc acgtggccgg
     1321 aagagagcgg cggcgcgcta tcgaccaccg tcccgacgac cttgggaatg gtgccaacgc
     1381 caccggtgcc ggctgtgctt ccggtcgacc cggtcgccgt ctggccggcc tgctggccct
     1441 cggtaccatg ctcactgacc gaatcgcctg tttccgggtc ggcgatcgcg accgcggacc
     1501 cgaatcctgc ggccagcagg gcgacggaca ggacgcagat ccctgccgcc aagcgccttc
     1561 gcgtactcat ggtgtttctc ctgccacccg ccgggagagc ccgctacacc tatttccgtc
     1621 actatcctcc cattgcgcgg tcctggcgtc gattccttca gaaatccaac tcagccgccg
     1681 gtgacggacg ggaccgacga tgttgacggc gactgatgga cacgcaggtc gtagcagcgg
     1741 gccgcagctg acgatttttg cgccactgct cacagccttg ggcacagcag tggtcgccta
     1801 cggccttctg agcggctctc tgacaaagaa gcagctccga tggcaacgcg agtcgccgag
     1861 cccacccgca ctggcgcccg atccaccgat tccggccgac gtcagctgat agaggtcggt
     1921 cgcgctaaag gtcgccttcg aatcgattct gcattaaccg tagggctacg ggagcggcac
     1981 gctccgtgat gatcatcggc gacggtgtgt cgtcttccca gtaccgggcg gtggtgggtc
     2041 gcggctgacg cggtggtcac ccgctagacg tggtaaccga ctgaagagcg tcgctacagc
     2101 gggtcgtgac gccgccgacg caaaggcccg cgagatgcct tgtgtcccaa gcgtagtaag
     2161 ccaacctcgt cgtccccatg gcaagtactc accacgccgc gtcggtcgga gcttcccata
     2221 tgccggactg gccgatcggc gatgtcgcgg tttcgtctcg tgcgcaaccg aggctcggat
     2281 agcgcttctc cagctcggcc agtgcgcgaa acgccgtcac aggcgatggc agcgacacct
     2341 catccgccgg gcgacgatcg caccgatcta ggcagcccct tagcggttcg cagtgccatc
     2401 ggcgaggtcg gggtggatgc ccgggatgag gtctgcgccg ccggcgatcc gctgcaggag
     2461 ttcgcgcagg gtatttcgtt cgattgccga taggggtgcg gtgatggtgt cttcgagtgc
     2521 ccggctggcc ttgtcgaggt cgggcagcag gctgcgggcc cggtccgtga gagtcaccgc
     2581 gaatgcccgg cggtcgtcgg ggtgcgccat acgtgtgacc agcccgtcgg cttcgagggc
     2641 gtcgatgacg gtcaccattg cgttgcggtg gatgccgagt ccgacggaga gctgccgctg
     2701 agactgtccg tcttcggtcg ccaaggccat gagcaccgcg taggtccggg gtcgcacacc
     2761 gacgccggcc atccggcgct gaaactcgcc cgcgacgtgg gagccgagct gggacagcag
     2821 gaacgggatg cgttcgtcaa gagcggcaat gcccgtcgac cgagccttca tgcacgaata
     2881 ctagcagtcg catatgattg tcatgtattg tgatgatcac caaccgtgat tgattggaga
     2941 ggtgtcatga gactggcaat attcggcgcc aacggtcaga ccggtcgcct agcgaccaca
     3001 cttgccctgg atgcgggcca ccacgttgta gcagtgaccc gtcggccgcg tgaattcccg
     3061 ctgaccgcac cgggtttgac ggtcacgggg gcggatgtgc gggatgcgtc cgcagtcggg
     3121 acggtcgtgg ctgaggtcga cggggtcgtg tccgcgcttg gtgtcacgtt cacccgtgag
     3181 caggtcgaca cgtattcggt ggggacgggc aacgtcgtgg ctgccatgca tgccgctggg
     3241 acgcggcggc tcgtggtggt cagctcgacg gccgcgtacc ccactcgccg cagccacgtc
     3301 ccgttcatgc tccggttcgt ggagccgatc atcggcaaca cgatcggcaa gaccgtgtac
     3361 gacgacatcc ggcgcatgga gagcgtcgtg cgtgacagcg gcctggattg gacgatcgtt
     3421 cgcccctcgg gcttgttcga cctgcccgaa cccaccaact acgtccgagg ggaggtcgat
     3481 cccgtcggcg cgttcaccgc gcgcatcgac cttgccgatt atctggtgtc gctggcgtct
     3541 gccgcgacag gcgcgcgccg gaccgtcatt gcctcgacca cggaaagcac tccgacactg
     3601 tggcagatga tccgccgcga agcgggctcc cgcgacagcg aaagaagggc tgtcagttga
     3661 cgcgccagaa gacgaccgcg cacacccaca ccccgatcgc cgaccctgcc gatccacccg
     3721 gccggcacgg tctgctccag agggcatcgg ccttcgccgc ggtgttgttc agtggcctgt
     3781 tcgccggatt cctcatcacc gtcctcgtcc tcgaagaaag cctgcgtagt tccggtgcgg
     3841 cggtctacac ccaggtccgc ttggtcgaac tcgatcacct cgacgacctg gccaccgcgc
     3901 tgctaccacc agccactctc gccgctgcga cgctgacctt ggccctgatc cgctcgcgcg
     3961 gtagcgcccg ctggctcaca ctggccgcag taattctgtt actggcgacc gccgcgatca
     4021 gtgtgtcgat caacgtgcca atcaacaccg atcagctgaa ctggccggtc ctgtccccgc
     4081 cgcacgactg gtcaaatgtc cgtgaccgct ggcaactggc ccacgcaaca cgtaccaccg
     4141 ccgccacact ggccttcgtg ttactgacgg cagcagtggt gcccctgcgc ttgccccgaa
     4201 ccgcggtcga actctccggc gagaaccagc agacaggcta gagcctgtgt ggcagaaggt
     4261 ccgccgggaa gccactgctc aaccggccga aacggcgcac gcttactagc gggcgaccgg
     4321 gtcgattctc ggcagcgacc gacaaccctg catacattcc cagccacgtg accaccgcgg
     4381 cacatccgga caacctgcga aaggaccatc cgtgatactc gtcaccggcg cgaccggcac
     4441 catcggccgt cccctcgtcg agctgctgac cgcccagggt gcggaggtgc gcggcctcgc
     4501 gcgccatacc ggcgatgatc tcgacgccct accgggcgcc gtcgagatgg tcgccggtga
     4561 tctgtcgcgg cccgagacga tcgtggctgc actggacggc gtcagttcgt tgttcgtcca
     4621 tccacgtgcg gtgggagacg ctgcccctgc gctgctagcg ttggccgccg agcgcggagt
     4681 cgaacacgtc gccgtactgt cggcgatcaa cgtcgatgac gatctttcct ggcaaccgtc
     4741 gcggctcaac ggtgaccgca acaaagaggt cgaagacgcg gtcattggca gcggcatggc
     4801 ctgggtgagc gtgcggccct gttcatttgc ggccagcacg tcaagtatgt gggccacaca
     4861 gatccgaaac ggtgacgtcg tacgcggtcc acatgctgag ttcgccgacc cgttcatcca
     4921 cgaagccgac gtggctgcgg tgctcgccgc ggcgttgctc gatcaccgcc ttgccggcca
     4981 aaagatctcg atcaccggcc cgcagtccat cacccacact cagatggtga ccaccatcgg
     5041 tgcggtgatt ggcaggccgc tgcgctatga ggagatctcg cctacccaag ccgcggacga
     5101 catcgttgcc ctcgggctac cacgctcctt cgccgaggcg atgatggccc gctactcccg
     5161 cgacctcgat cggccgaccc cggtcaccgt cggggtgaag aagaccctca gccgcccggc
     5221 ccgcacctac gcggaatggg tcttcgatca cgcaacagcc tttcgaaatg cgcctgcggc
     5281 aacgcaaacg agctaggaac gaactctcac gccctccgaa ttcgaaccta tcgaactagg
     5341 ccgaagccat cacgatccgg tagtcattga ttgagcaggg gggccagtcg ccgggcggcc
     5401 tccagtggac ccggctgacc tgtgctgtga aggacggtga actgaacgtg gtcggctccg
     5461 gcggcgcgct gttcgcgtgc tcgcgcaacg atgtcaagag ggctgcccca ggcgaccagc
     5521 gcgtcgacga gtcgatcact caacacgctg atgtcgtcat cggtgaaccc ctgccgacgc
     5581 gccgagttcg catagccgcg cacctcgtgg aggaggaacc gcagcggcgt ccgagccgtc
     5641 tcccgggcgc tactcgggtt ttcgtcgagt accacgaact ggccaaccgc caaagtgctg
     5701 ttggggccga gccgttcgcg cgccgtggcg gtgtacgcgg gtttcacgag tatgggaatc
     5761 gcaccggcga actggtcgcg ggcgatgtca agtttgcgcg gaccgatggc tgcaagcatt
     5821 cgacgttcca ggggaacagg atgcgcggcg gcggtcagct cgtcgaggta actgtggagc
     5881 gcggagatcg catgcggcac ctgcggggcc cccaaaccga ccagcagccg gccgggacgg
     5941 gttgtctcca ttgactggta caggtccaga acttctctcg cgtcatacac atcgggcgag
     6001 attatggacg gcgcaacgat gacatgatca gtcgcgcgga gcagatcagc aagctgctca
     6061 agacgatcca gctgcccacc tgccatccac aacgcggtga agccgagact ctcgatctcg
     6121 gccgcagcca ccaaatgggc gtcaccggcc tcggtttcga tcgcaaagcc ccatgcaccc
     6181 aggttcatct tcaccgacac gagcctaaaa cctcaacttc actcaaggtc aagcgccgcc
     6241 gcctacaacg ccaggcgctc gcccgacggg ggacacggac cgcaccacgt ccggtcgata
     6301 gtcgagcacg tcacataatg taccacttga tgattgtcac tttatgtgac agtctagcca
     6361 agtgactaac tttggcccgg ctacgagacc cgaggaaatg ctcgactggg acagcgttta
     6421 caaacaggaa tccgatgtca tacaggggcc ggctccatgg aacatcggcg agccccaacc
     6481 agcgctcgcc gaactgattc ggcagggcag gtttcggggc gacgtactcg acgcgggatg
     6541 gggatttgcc gaagtgtcac tgtgtttggc cgcagaggca tacaccgtgg tgggtatcga
     6601 tatcaaaccc accgcggtcg ccgcggccac cgcgatagcg gccgagcgcg gcctatccac
     6661 cgcgaccttc ctgcacgccg acgccacgtc gtttaccggc tacgacggcc ggttctccac
     6721 cgtggtggac agcacgctgt tccattccct tcccgtcgaa ctccgtgacg actacctgaa
     6781 ggcggtctac cgggcggccg ccccgggcgc gtcgtatttc atcctggctt tcgccaaggg
     6841 cgcgttccca gcggaattgg ccgccaagcc caacgaggtg accgacgacg agctgcgggc
     6901 ggccgtcgcc aagtactgga tcgtcgacga cattcgcccg gcatcgatat acacgatgct
     6961 gccccagtac agcagcgcgg cgcggccagt ctcattcccg ccgttcgaga ttgacgagaa
     7021 gggccggccg aagctcgcgg cgtggctgct cactgcacac aagccgggct aagacgagcg
     7081 agctcctcgg cggaccgcac atgaccaaga cctgattgtc ggcagaagaa ccggcgatgg
     7141 caattgaccc aaggaggcac cgcaatgaag atcaccgtgt tcggcgcgac aggcggtatc
     7201 ggcagacaac tggtcgcaca agcactgaac cacgggcacg acgtgaccgc ggtggttcgc
     7261 gacgctgccc gcttgcccat cagcgacgtc gcccttcatg tagtaaccgt gcccgggctc
     7321 gagcagcccg acctactgcg ttctgcgctc attggcagcc acgcggtcct ctcggcggtg
     7381 ggcccacgag gccgcaagga cgggccggtg gcctcgacgg ctgtccgcac catcctgaac
     7441 gcgatggact ccgcgggagt acgtcgattg gtcgcagtga gcgcagcccc ggtcgggccc
     7501 gtccctgacg gcgagagcgc gctcaatcgc tggctgctct tgccactcgt cggccggctg
     7561 ctgcgaagcg tctacacgga tctgggcgag atggaggccc aattgctccg cagtagcgcc
     7621 gcgtggactg tgattcggcc gccaaagctc gtcaacaaac cgctcaccgg catctaccga
     7681 accaccttgg ggggcaacgt tccccgcggc atctcgatat cgcgggctga cgccgctcac
     7741 ctgatgctcg ctgcactgga tcaacccgcg acggtcacac aggccgtcgg cgtggcctac
     7801 tgacaacact tacaaaagga gtatcgacca tgaccaccgc atacgtcgtc gtgatactga
     7861 tcgccgttgc ggccaacggc ttttcgggga ttgccgctct gctgcacatg aaggccattc
     7921 ttcccgggat ggcgaaagcc ggcgtgcccg aatcgtggct gaccttcccg atcggaacgg
     7981 ccaagaccgc cggtgc
//
LOCUS       DASYLX010000409         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2871725,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000409 DASYLX010000000
VERSION     DASYLX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2871725"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGP27_10085"
     CDS             complement(<1..386)
                     /locus_tag="VGP27_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEV7851698.1"
                     /translation="MLASVIAVALVLVGWSLLARRLERWRVTAPMFLVLAGIAVGFST
                     HDVLVESLNTDAAQRAAEIILAVLLFVDATDIRGGLFGSQPRAAVRVLFIAMPLSLAL
                     SVALGLWLLPGLGWAVLLVIACVVVPT"
     gene            complement(416..760)
                     /locus_tag="VGP27_10090"
     CDS             complement(416..760)
                     /locus_tag="VGP27_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7851699.1"
                     /translation="MALKTENITIDTTDPEKLADWWARALDGQLNPVAPPFFVVVSLP
                     EGPGLAFQKVDDPTPGKNRVHVDFSTSDMEAEVKRLVGLGASETGRGNFGDFEWVVLA
                     DPDGNAFCVAGG"
     gene            complement(811..2070)
                     /locus_tag="VGP27_10095"
     CDS             complement(811..2070)
                     /locus_tag="VGP27_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HEV7851700.1"
                     /translation="MSASAQGLCEFIDASPSPFHVCVTVAQRLVDAGFRELAEADAWP
                     TFGDFFTVRAGSLVAWRAAGADPAAPFRIVGAHTDSPNLRVKQHPDRFVSGWQVVALQ
                     PYGGAWLNSWLDRDLGISGRLSVRTADGIEHRLVRIDEPVLRVPQLAIHLAENRKSVS
                     LDPQRHVNAVWGVGSGPRSFLGYVAGRAGVDADAVLGIDLMTHDLTPSTLAGAEGELL
                     SAPRLDNQCTCYAGLEAFLAAEPAGRLPVLALFDHEEVGSQSDHGAQSELLLTVLERI
                     TLAVGGGREDFLRRLPGSMVASGDMAHATHPNYPERHEPYHLIEVNGGPVLKVQPNLR
                     YATDGRTAAAFALACGQAGVPLQRYEHRADLPCGSTIGPMTAARTGIPTVDVGAAQLA
                     MHSAREVMGADDVASYSAALRAFLAPG"
     gene            2113..3141
                     /locus_tag="VGP27_10100"
     CDS             2113..3141
                     /locus_tag="VGP27_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEV7851701.1"
                     /translation="MPAVLPQPVLAPLTPAAVFLVVTIDEGGESTVHDALPDISGLVR
                     SIGFRDPSKRLSVITSIGSDAWDRLFSGPRPAELHPFVEIQAGRHHAPSTPGDLLLHI
                     RAESMDVCFELATKLVESMDGAVTVVDEVHGFKFFDNRDLMGFVDGTENPDGPLAVSA
                     TQIGDEDAAFAGGCYVHVQKYVHDMQSWNSLSTEEQERVIGRTKLDDIEMDDAVKPAN
                     SHIALNVITDADGNELKIVRHNMPFGEIGKGEFGTYYIGYSRTPAVTERMLDNMFVGD
                     PPGNTDRILDFSIALTGSMFFTPTVDFLDDPPPLPDSAPEEEPADVVVAPASRDGSLG
                     IGSLKGLE"
     gene            3142..3939
                     /locus_tag="VGP27_10105"
     CDS             3142..3939
                     /locus_tag="VGP27_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEV7851702.1"
                     /translation="MNNLYRDLAPVTESAWAAIEMEATRTFKRHVAGRRVVDVSEPGG
                     PTTAAISTGHLLDVGAPGDGVIAHLRESKPLVRLRVPFTLSRNEIDDVERGSQDSDWD
                     PVKEAAKKLAFVEDRAIFEGYQDASIGGIRGCSSNPALALPSDAREIPDAIAQALSEL
                     RLAGVDGPYSVLLSADVYTKVSETTEHGYPIREHLNRLVDGDIIWAPAIDGAFVLSTR
                     GGDFDLQLGTDVAIGYLSHDAETVRLYLQETLTFLCYTAEASVALTP"
     gene            complement(4067..>4193)
                     /locus_tag="VGP27_10110"
     CDS             complement(4067..>4193)
                     /locus_tag="VGP27_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV7851703.1"
                     /translation="SNGRVVGLMPHPEHATEALTGPSDDGLGLFYSALDSVLANA"
BASE COUNT          713 a         1437 c         1396 g          647 t
ORIGIN      
        1 gttggcacca caacgcacgc gatgaccagc aggaccgccc acccgaggcc cggcagcagc
       61 cacagtccca gggctaccga caacgccagg ctcagcggca tcgcgatgaa cagcacacgc
      121 accgccgcgc gcggctgact cccgaagagc ccgccgcgaa tatcggtggc gtcgacgaaa
      181 agtaggacag ccaggatgat ttccgcggcg cgctgagcgg cgtcggtgtt cagcgactcg
      241 accagcacgt cgtgggtgga gaagccgacc gcgatgccgg cgagcaccag gaacatcggc
      301 gcggtgacgc gccagcgctc gagccggcgg gccagcagcg accacccgac caggacgagc
      361 gctaccgcaa tgaccgacgc cagcacgggc gcgattgtgc ccggtgcgcc ggtcgttagc
      421 cgcccgccac gcagaacgcg ttgccgtcgg gatcggcgag caccacccat tcgaaatcgc
      481 cgaagttgcc tctgccggtt tcgctggccc cgaggccgac cagccgcttg acctcagcct
      541 ccatgtcgga ggtgctgaag tcgacgtgca cgcggttctt acccggcgtg ggatcatcga
      601 ccttctggaa cgccagaccc gggccctctg gcagcgacac gacgacgaaa aagggtggag
      661 ccacagggtt tagctgcccg tcgagtgcgc gggcccacca gtcggccagc ttctcggggt
      721 cggtcgtgtc gatagtgatg ttctctgtct tgagcgccat gcggtgacct taggccagcc
      781 ctgtgacaaa tcggggagat caccgtcggc ttagccaggc gcgaggaagg cccgcagcgc
      841 agccgagtac gaggcgacgt cgtcggctcc catcacctca cgggccgagt gcatggccag
      901 ctgcgcggcg ccgacgtcga cggtgggaat gccggtgcgc gcggcggtca tcggtccgat
      961 cgtcgacccg cacggcaggt cggcccggtg ctcgtagcgc tgcagcggga caccggcctg
     1021 cccgcatgcc agtgcgaacg cagcggcagt gcggccatcg gtggcgtagc gcagattcgg
     1081 ctgcaccttg agcaccggcc cgccgttgac ctcgatcagg tggtagggct cgtggcgctc
     1141 cgggtagttc gggtgggtcg cgtgcgccat gtcgcccgac gccaccatcg agccgggcaa
     1201 ccggcgcagg aagtcttcgc ggccgccgcc cacggccagc gtgatgcgct cgagcaccgt
     1261 cagcagcagc tcggactggg cgccgtgatc cgattgggag ccgacttctt cgtggtcgaa
     1321 gagcgccagc accggcagac gacccgccgg ctcggccgcc aggaacgcct cgaggcccgc
     1381 gtagcaggtg cactggttgt ccagtcgtgg cgcgctgagg agctcgccct cggcgccggc
     1441 cagcgtcgac ggggtcaggt cgtgggtcat caggtcgatg ccgagcaccg cgtcggcgtc
     1501 cacgccggcc cgcccggcga cgtagccgag gaacgaccgc ggtccgctgc cgacccccca
     1561 gacggcgttg acgtgccgct gcgggtccag tgacaccgac tttcggttct cggccagatg
     1621 gatggccagc tgcgggacac gcaggacggg ctcgtcgatc cgaaccagcc ggtgctcgat
     1681 tccgtcggcg gttcgcaccg acagcctgcc gctgatgccg aggtcacggt cgagccacga
     1741 gttcagccac gccccgccgt acggttgcag cgcgacgacc tgccagcccg acacgaaccg
     1801 gtccggatgc tgcttgaccc gcagattggg gctgtcggtg tgggcgccga cgatccgaaa
     1861 cggcgcagcg gggtcggcgc ccgccgcccg ccatgcgacc agcgatccgg cccgcacagt
     1921 gaagaagtcc ccgaaggtcg gccaggcgtc tgcctcggcc agctctcgga agcccgcatc
     1981 cacaagtcgc tgtgcgaccg tcacgcaaac atgaaacggc gacggcgagg cgtcgatgaa
     2041 ctcgcacaaa ccttgtgcac ttgccgacat ggttccccat cctcgcccac gaccttgact
     2101 tagggttcaa cggtgcccgc tgttctaccg caaccggttc tggcgccgct gactccggcg
     2161 gccgtcttcc tggtggtcac gatcgacgag ggcggggagt cgaccgttca cgacgcgctg
     2221 ccggacattt cgggactggt gcgctcgatc ggcttccgcg acccgtcgaa aaggctgtcg
     2281 gtgatcacct cgatcgggtc cgacgcgtgg gaccgactgt tctccggacc acgcccggcc
     2341 gaactgcacc cgttcgtcga gatccaggcc ggacgtcacc acgcgccctc gacgccgggg
     2401 gatctgctgc tgcacatccg ggccgagtcg atggacgtct gcttcgagct ggccaccaag
     2461 ctcgtcgagt cgatggacgg cgccgtcacg gtggtcgacg aggtgcacgg cttcaagttc
     2521 ttcgacaacc gcgacctgat gggcttcgtc gacggcaccg agaacccgga cgggccgctt
     2581 gcggtgtccg ccacgcagat cggtgacgag gatgccgcct tcgcgggcgg ctgctacgtg
     2641 cacgtccaga agtacgtgca cgacatgcag tcgtggaact cgctgtcgac cgaggagcag
     2701 gagcgggtga tcggtcgcac caagctcgac gacatcgaga tggacgatgc ggtcaagcct
     2761 gccaactcgc acatcgcgct caatgtcatc accgacgccg acggcaacga gctcaagatc
     2821 gtccgccaca acatgccgtt cggtgaaatc ggcaagggcg agttcggcac gtactacatc
     2881 ggctattcgc gcaccccagc ggtcaccgaa cggatgctgg acaacatgtt cgtcggtgat
     2941 ccgccgggta acaccgaccg gatattggac ttctccatcg ccctgaccgg aagcatgttc
     3001 ttcaccccga cggtcgattt cctcgacgac ccaccgccgc tgccggattc ggcacccgag
     3061 gaggaacccg ccgacgtcgt cgtcgcaccc gcatcgcgtg acggatcact cggcatcgga
     3121 agcctgaaag gacttgagta gatgaacaac ctgtaccgcg acctggctcc ggtcaccgaa
     3181 tcggcttggg cggcaatcga aatggaggcg acgcggactt tcaagcggca cgtcgccgga
     3241 cgacgcgtcg tcgatgtcag cgagcccggt ggccccacca ccgcggcgat cagcaccggg
     3301 cacctgctcg atgtgggggc accgggcgac ggtgtgatcg cccacctccg cgagagcaag
     3361 ccgctggtcc ggttgcgtgt tccgttcaca ctgtcgcgca acgagatcga cgatgtcgag
     3421 cgcggttcgc aggactcgga ctgggatccg gtcaaggagg ccgcgaagaa gctggcgttc
     3481 gtcgaagacc gtgcgatctt cgaggggtat caggacgctt cgatcggcgg catccggggc
     3541 tgcagctcca acccggcgct cgcgctgccc agtgacgcgc gagagatccc cgacgcgatc
     3601 gcccaggcgc tgtccgaact gcggctggcc ggcgtcgacg gcccgtattc ggtgctgctc
     3661 tctgcggacg tgtacacgaa ggtcagcgag accaccgagc acggctatcc gatccgcgaa
     3721 cacctcaacc gtcttgtcga cggcgacatc atctgggcgc cagcgatcga cggcgcgttc
     3781 gtgctgtcca cccgcggcgg cgatttcgat ctgcagctgg gcaccgacgt cgcgatcggg
     3841 tacctgtcgc acgacgccga gacggtgcgg ctctacctgc aggagacgct gacgttcctc
     3901 tgctacaccg ccgaagcctc cgtcgcccta accccctgac ctttggcccc gcgagcgtgc
     3961 gtgttggcac acgacacgcc gaccgaaatt ggcattcttc gcacgctcgc ggtgtgtcga
     4021 cagcgacggc ggggccctcc ggcccgttgc ctaggtgtga tgttgcctag gcgttggcca
     4081 gtaccgaatc gagcgcggag tagaacaggc ccagcccgtc atcggacggc ccggtgagtg
     4141 cttcggtcgc gtgttctggg tggggcatca agccgaccac ccgcccgttc gac
//
LOCUS       DASYLX010000410         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1555894,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000410 DASYLX010000000
VERSION     DASYLX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555894"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGP27_10115"
     CDS             complement(<1..285)
                     /locus_tag="VGP27_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV7851704.1"
                     /translation="MTQDTSDASPVTSGTPPAAAGCPVSGGGYDALQAPLGPDSLTWK
                     YFGQWTGMLQGSWAGSMQNMHPQLGAAVKDHSIFFMERMPRLYRSVYPIGG"
     gene            356..949
                     /locus_tag="VGP27_10120"
     CDS             356..949
                     /locus_tag="VGP27_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851705.1"
                     /translation="MAAGQRRGRWSGVPLEHRQALRRGELIDAGVALLGGESGPALTV
                     RAVCRVAGLTERYFYESFPDRDEFVRAVYDDVCARAMSTLSSSTSAHDAVERFVALMV
                     DDPTRGRVLLLAPTVEPVLTRSGANWMPSFIDLLQRRVTRITEISQPAVQAMAATGLL
                     GALTALFIAYLDGRLSATREQFIDHCVDMVLNRVSSF"
     gene            complement(988..1467)
                     /locus_tag="VGP27_10125"
     CDS             complement(988..1467)
                     /locus_tag="VGP27_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851706.1"
                     /translation="MKKILISASTVGAAGIAMGALGLFGAGTAAAAPDVVGMTYGDAV
                     TAIEDGGGTAKISVTVGDRQDAMGDCLVTNASDAPFVRDSDGSFAHADGEVLLALNCN
                     RGAATGTTPGASAASPEGRTFTAKAEEAAAQAQSAQGELAQAGATPGDTTNADIPTG"
     gene            1780..>2231
                     /locus_tag="VGP27_10130"
     CDS             1780..>2231
                     /locus_tag="VGP27_10130"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEV7851707.1"
                     /translation="MHENLTDLHLLHELEPVVEKNLNRHLSMRKDWNPHDYIPWSDGK
                     NYYALGGEDWEPGQSALSEVAQTAMVQNLLTEDNLPSYHREIAMNFGMDGAWGQWVNR
                     WTAEENRHGIALRDYLVVTRAIDPVALETLRLEQVTRGFSPGQNQQGDL"
BASE COUNT          388 a          743 c          721 g          379 t
ORIGIN      
        1 gccgccgatc ggatacaccg accggtacaa ccgcggcatc cgctccatga agaagatcga
       61 gtggtccttg acggcagcac ccaactgggg atgcatgttc tgcatcgaac ccgcccagct
      121 gccctgcaac atgccggtcc attggccgaa gtacttccag gtcagcgagt cgggcccgag
      181 cggtgcctgc aaggcgtcgt agccgcctcc cgacacgggg caaccggcgg ccgcaggtgg
      241 ggttccgctg gtcaccgggg aggcgtcaga agtatcttgg gtcactggtg ggcttccgat
      301 cggtgtcaga cgcaattctg actacgtacg ttgtcaccat gattctagga gtgatgtggc
      361 tgcgggtcaa cgacggggcc gatggtcggg cgtaccgctc gagcaccgtc aggcgctgcg
      421 ccgtggcgag ttgatcgacg cgggcgtcgc cctgctcggc ggtgagagcg gtccggcact
      481 caccgtgcga gcggtgtgca gggtggccgg gcttaccgaa cgctacttct acgaaagctt
      541 tcccgaccgc gacgaattcg tccgcgcggt ctacgacgat gtctgtgcgc gggcgatgtc
      601 caccctgtcg tcgtccacca gcgcccacga cgcagtcgaa cggttcgtcg cgctgatggt
      661 cgatgacccg acccgcggca gagttctcct gctggcgccg acggtcgagc cggtgctgac
      721 gcggtcgggc gcgaactgga tgccgagctt catcgatctg ctccaacgca gggtcacccg
      781 gatcacggag atcagccagc cggcagtcca agctatggcg gccaccggcc tgctcggcgc
      841 gctgacagca cttttcatcg cctacttgga cggccgcctg agcgctaccc gcgagcagtt
      901 catcgaccac tgcgtggaca tggtgctcaa cagggtgtcg agcttctgag acctacccgc
      961 ccgccacacg gtccacggcg tgcgtgacta cccggtcggg atgtccgcgt tggtggtgtc
     1021 gcctggggtt gcgccggctt gcgcgagttc accttgcgcg ctctgcgcct gggcggcggc
     1081 ctcttcggcc ttcgcggtga aagtgcgacc ctcggggctg gccgccgagg cgccgggcgt
     1141 ggtgcccgtc gcggctccgc ggttgcagtt cagcgccagc agcacctcgc cgtcggcatg
     1201 cgcgaatgag ccgtcggaat cgcggacgaa cggcgcatcg ctggcgttgg tgaccaggca
     1261 gtcgcccatc gcatcctggc gatcgccgac ggtgaccgag atcttcgccg tgccgccccc
     1321 atcctcgatc gcggtaacag catcgccgta ggtcattccc accacgtccg gcgcggcggc
     1381 ggcggtgccc gccccgaaca gtccgagggc gcccatcgca atccctgcgg cgccaacggt
     1441 gctggccgaa atgaggatct tcttcacatg cccacctatg tctaaggtct acggttgccg
     1501 agtgtaggcg cggttcgatt tcttgtgaac tgcctgcggg aaccataccg ccgaccggcg
     1561 caacccgcga gcaataccgc cgcgtagggc aggcctgaaa ccgggtgccg accggggccc
     1621 gaatcgtgac ctttccgacg cagcgacaca tatcgtgcaa cttgatgtca ccgggttaca
     1681 cagatatatt cactgcaacc aacaggtaca gataaatcta agaggccttc gtccaccgaa
     1741 tgacggcagg tcccgcccct aaaggagaac gccgggctga tgcacgaaaa cctcaccgac
     1801 cttcacctgc tgcatgagct tgagcccgtc gttgagaaga acctcaaccg gcacctgtcg
     1861 atgcgcaagg actggaaccc tcatgactac atcccgtggt cggacggcaa gaactattac
     1921 gccctcggcg gcgaggactg ggagcccgga cagtcggcgc tctccgaggt cgctcagaca
     1981 gccatggtgc agaacctgct gaccgaggac aacctgccgt cctaccaccg cgagatcgcg
     2041 atgaacttcg ggatggacgg ggcttggggc cagtgggtca accgctggac cgccgaggag
     2101 aaccggcacg gcatcgcgct gcgcgactac ctggtcgtca cccgcgccat cgatccggtc
     2161 gcgctggaga cactgcgcct cgagcaggtg acccgcggct tcagcccggg ccagaaccag
     2221 cagggcgatc t
//
LOCUS       DASYLX010000411         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_238836,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000411 DASYLX010000000
VERSION     DASYLX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238836"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="eccD"
                     /locus_tag="VGP27_10135"
     CDS             <1..661
                     /gene="eccD"
                     /locus_tag="VGP27_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEV7851708.1"
                     /translation="AVAAAVVAIWPAPTASVGAVLTAASLAMMGVAAKLSIFLVGLAP
                     RMPGTNGADDEEAIPVDVGACCADRGHNALTGLLAGFSLSTALGTVLVAADHHRPNTW
                     SGVALTAAASVVLMFRATQQRGAVRSAAVLAAGLVSTTAALVLTALSAPQHRILVSLI
                     AVAFGFGGLWTVRADLGSSLSPPARRGLEVMDYLAIAAVVPLACWVAGVFGFVRGLSL
                     T"
     gene            658..2058
                     /gene="mycP"
                     /locus_tag="VGP27_10140"
     CDS             658..2058
                     /gene="mycP"
                     /locus_tag="VGP27_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEV7851709.1"
                     /translation="MTSRLARLTTAVMLASIPVWASPVAAAVMPPAVDDSLLPAPRPP
                     AAPEATEQRQQCVVSAAVTAGDPRAARSQLKGLNLPAVWQLSRGAGQTVAVIDTGVAR
                     HRLLPHLVAGGDYVFTGDGTDDCDGHGTVVAGIVGATEQATEDGGFSGVAPDATIIGI
                     RQSSVKFAPAGQSAPGFGDVDTLAMAVRTAADMGATVINVSSIACVAAAAALDDRPLG
                     AALAYAVDVKNVVVVAAAGNVGGLGQCPDQNPPADPARPGMPDWESVKVVVSPAWYDD
                     YVLTVGSVDPEGAPSRFTLAGPWVDVAAPGESVVSLDADGEGLVDSLPVLGDAMPIMG
                     TSYAAPVVSGIAALVRARSPQLTARQVMQRIKDTAHRPPAGWDPFVGHGIVDALAAVS
                     GGGATTDMTIRESESSDGGVRPVSPPESEIKPDQSGRRIAFGGAGICVAVISGAVAMT
                     ASLGRLRRQGKTIARD"
     gene            complement(2023..>2656)
                     /gene="eccB"
                     /locus_tag="VGP27_10145"
     CDS             complement(2023..>2656)
                     /gene="eccB"
                     /locus_tag="VGP27_10145"
                     /inference="COORDINATES: protein motif:HMM:NF016964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEV7851710.1"
                     /translation="PVGTVVRVTRVDGDDYFAVLADGVQRIGEVAANLIRTIGPRASR
                     EIATVSPDAIGDVPVVDSLPVSSYPTRAGMTIGAGDTGVICAQTLPYGPETILWQGDS
                     LPVDPSPVDLAQRDGEGPNIDSVVLPSGRSAYVRSAALTGEGGAAGPLYLVTDSGVLF
                     GLRDEDTAKLLGVRGNPVAAPWPVLSRLPRGPELSREGALVARDGLALPP"
BASE COUNT          390 a          849 c          940 g          477 t
ORIGIN      
        1 ggcggttgcc gcagcggtgg tggcgatttg gccggcccct actgcctctg tgggcgccgt
       61 gctgaccgcc gcatctctgg cgatgatggg tgtcgcggcg aagctgtcga ttttcctggt
      121 cggtctggcg ccgcggatgc cgggtaccaa cggtgcagac gatgaagagg ccattccggt
      181 cgatgtcggt gcgtgttgcg cggaccgcgg ccacaacgcg ttgacgggac ttctcgccgg
      241 cttctcgttg tcgacggcac tggggactgt gttggtcgcc gcggaccatc accgtccgaa
      301 cacgtggagt ggtgttgcgc ttacggcggc tgcctcggtg gtgctgatgt ttcgggcaac
      361 tcaacagcgg ggagcagtcc ggtccgcggc cgtgcttgcg gctggactgg tcagcaccac
      421 cgcggctttg gtgttgaccg cgctgtcggc gccgcagcat cgcatcctgg tgtccctcat
      481 cgcggtcgcc ttcggtttcg gcggcctgtg gaccgtgcgt gcagacctcg gatcatcatt
      541 gagcccgccg gcgcgtcgcg gcctcgaggt gatggactac ctcgccatcg cggcggtcgt
      601 accgttggct tgttgggtcg ctggtgtctt cggcttcgtc cgcggcttga gcctgacatg
      661 acctctcggc tggcgcgact caccacggcc gtcatgctgg cgtccatccc ggtgtgggca
      721 tcacccgttg ctgcagcggt gatgccgccc gcggttgatg actcgctact gcccgcaccg
      781 cgaccacccg ctgcgccgga ggccaccgag cagcgccaac agtgcgtcgt ttcggctgcc
      841 gtcacggcgg gcgatccaag ggcagccaga agccagctca aaggtctgaa tctcccggcg
      901 gtctggcagc tcagtcgcgg ggccggtcag accgtcgccg tcatcgacac aggggttgcg
      961 cggcatcgcc tgctacctca tcttgttgcc ggaggcgact acgtgttcac cggcgacggc
     1021 accgatgact gcgacgggca cggaacggtc gtcgccggaa tcgtcggtgc aaccgaacaa
     1081 gcaacggaag acggtggatt cagcggggtc gcacccgacg caacgatcat cggcattcgg
     1141 cagtccagcg tcaagttcgc gccggccggc cagtcggctc cgggattcgg tgatgtggac
     1201 acgttggcga tggcggtacg gaccgcggct gacatggggg ccacggtgat caatgtgtca
     1261 tccattgcct gcgttgcagc cgccgcggcg cttgacgatc ggccgctggg tgcagcgttg
     1321 gcatatgccg tggatgtcaa gaacgtcgtt gtcgtcgcgg cagcgggaaa tgtcggcgga
     1381 ctcgggcagt gccccgatca gaacccaccg gcggatcccg ctcggcccgg catgccagac
     1441 tgggagtcgg tgaaagttgt tgtgagcccg gcctggtacg acgactacgt gctgacggtg
     1501 ggctccgtcg atccggaggg tgcgccatcg cggttcacgc tcgcagggcc atgggtggac
     1561 gtcgcggcgc cgggcgagtc cgtcgtttcg ctggacgcgg acggagaggg tctcgtcgat
     1621 tcgctgcccg tgctcggcga tgcgatgccg atcatgggga cgagttacgc ggcgccggta
     1681 gtgagcggaa tcgccgcgct cgttcgcgca cgatcgcctc agctgaccgc ccgacaggtg
     1741 atgcagcgga tcaaggacac tgcgcatcgg ccgccggcgg gctgggatcc cttcgtcggt
     1801 catggcatcg tcgatgcgct tgccgcggtc agcggcggcg gcgccaccac ggacatgact
     1861 attcgtgaga gtgagtcgag cgatggcggt gttcgtccgg tgtcgccgcc ggaaagcgag
     1921 atcaaaccgg atcaaagtgg ccggaggatc gctttcggcg gtgccgggat ttgcgtcgcg
     1981 gtgatatctg gagcggtggc gatgacggca tcgctcggcc ggttacggcg gcaaggcaag
     2041 accatcgcgc gcgactagtg caccctctct gctcagttcg ggcccgcgcg gcagccggga
     2101 cagcaccggc cacggagccg cgacgggatt gcccctgacg ccgaggagct tggccgtgtc
     2161 ctcgtcacga aggccgaaga gcaccccgct gtccgtcacc aaatacagcg gtccggcagc
     2221 gccgccttcg ccggtgaggg cagccgatcg cacgtaagcg cttcgccccg agggcagcac
     2281 gacgctgtcg atgttcggtc cctcgccatc gcgctgcgcc aggtcaaccg gactcggatc
     2341 gaccggcagc gagtcaccct gccagagaat ggtttccggc ccgtacggca gcgtctgggc
     2401 acatatcaca ccggtgtcgc cggcacctat ggtcatgccc gctcgggtgg gataactgga
     2461 cactggcagc gagtcgacga ccggtacatc gccgatggcg tcgggtgaga cggttgcgat
     2521 ctcacggctt gcccgcggcc cgatggtgcg gatcaagttc gccgcgactt cgccgatgcg
     2581 ctggacgccg tcggccagca cggcgaagta gtcgtcgccg tcgactcgtg tcacccgaac
     2641 taccgtcccg accggc
//
LOCUS       DASYLX010000412         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1334864,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000412 DASYLX010000000
VERSION     DASYLX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334864"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGP27_10150"
     CDS             <1..525
                     /locus_tag="VGP27_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HEV7851711.1"
                     /translation="AREMCSDVIDMNLDDPVAVVRDSTEGRGADSVIDAVGMEAHGSP
                     IAEAAQTAAAFLPSPIGRVMMKHAGIDRLAALNSAIAMVRRGGTISLSGVYGGAADPI
                     NMMSLFDKQVSLRMGQANVKRWVPDILPLLTDDDPLGTEEFATHRLPLNAAPDAYEKF
                     QKKQDGMIKVVLTP"
     gene            565..>1642
                     /locus_tag="VGP27_10155"
     CDS             565..>1642
                     /locus_tag="VGP27_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEV7851712.1"
                     /translation="MDQSSAPILDALVEYRKANRYGFTPPAHRQGRGVDHRVLSVLGL
                     EPFQDDLLATGGLDDRRSRGKFLQQAEDLMAEAVGADVAWFSTCGSSLSVKAAMMAVA
                     GGAEGGLLVSRDSHKSIVAGLIFSGVQPRWITPRWDAERHFCHPPSPKDVEEAWQRHP
                     DAAGALIVSPSPYGTCADLEEIAAVCHARGKPLIVDEAWGAHLPFHEDLPTWAMDAGA
                     DVCVVSVHKMGAGFEQGSVFHLQGDLIDKNRLSACADLLMTTSPNVLVYAAMDGWRRQ
                     MVEHGHELFDAALSLAENVRADIEQVPDLHVLDDELIGVEASHDLDRMQLMLDVSETG
                     TSGYQAADWLRENKQLDMHLADHRR"
BASE COUNT          286 a          533 c          550 g          273 t
ORIGIN      
        1 gcacgcgaga tgtgcagcga cgtcatcgac atgaacctcg acgaccccgt cgcggtggtg
       61 cgcgattcca ccgagggcag gggcgccgac tcggtgatcg acgccgtcgg gatggaggcc
      121 cacggttcgc cgatcgccga agccgcgcag acggccgccg ccttcctgcc gtcgccgatc
      181 ggcagggtga tgatgaagca cgcaggcatc gaccgtcttg ccgcgctcaa ctccgcgatc
      241 gccatggtcc gtcgcggcgg caccatctcg ttgtccggtg tctacggcgg cgccgcagac
      301 ccgatcaaca tgatgtcgtt gttcgacaag caggtgagtc tgcggatggg gcaggccaac
      361 gtcaagcgct gggtgcctga catccttccg ctgctgaccg acgacgatcc gctcggcacc
      421 gaggagttcg caacgcaccg cttgccgctc aacgccgcac cggacgcata cgagaagttc
      481 cagaagaaac aggacggcat gatcaaggtg gtgctgacgc cctgaccggc ggttgaagcc
      541 gtaacgctgg ggtactactc cgctatggat caatcctcgg cgccgatcct ggacgcactg
      601 gtcgaatacc ggaaggccaa tcgctacggc ttcaccccgc ccgcccatcg gcagggccgc
      661 ggcgtcgacc accgcgtgct gtcggtgctg ggccttgaac cgttccaaga cgacctgctg
      721 gcaaccggcg gtctggacga ccggcgcagc cgcggcaagt tcctgcaaca ggccgaggac
      781 ctgatggccg aagccgttgg cgccgatgtg gcgtggttct ccacctgcgg cagttcgctg
      841 tcggtgaagg ccgccatgat ggcggtcgca ggcggcgccg agggcggcct gctggtcagc
      901 cgggacagcc acaagtccat cgtggcgggc ctcatcttct ccggcgtcca gccgcggtgg
      961 atcacccccc gttgggacgc cgaacggcac ttctgccatc caccgtctcc caaggacgtt
     1021 gaggaggcgt ggcaacgcca tcccgatgcc gcgggtgcgc tgatcgtcag tcccagcccc
     1081 tacggcacct gcgccgacct ggaagagatc gccgcggtgt gccatgcccg tggcaagccg
     1141 ttgatcgtcg acgaggcgtg gggcgcgcat ctgccgtttc acgaagacct gccgacgtgg
     1201 gcgatggatg cgggcgccga tgtatgcgtg gtgagcgtgc acaagatggg cgccggcttc
     1261 gagcagggct ctgtcttcca tctgcagggc gaccttatcg acaagaaccg gctctcggcg
     1321 tgtgccgacc tgctgatgac cacgagcccc aacgtcctgg tgtacgcggc gatggatggg
     1381 tggcgacggc agatggttga gcacggccac gagctgttcg acgccgcatt gagtctcgcg
     1441 gagaacgtgc gtgccgacat cgagcaggtt cccgatctgc acgttctcga tgacgagctg
     1501 atcggcgtcg aagccagcca tgacctggac cgtatgcagc tgatgttgga tgtgtcggaa
     1561 accggcacat cgggttatca ggcagccgac tggcttcggg agaacaagca actcgacatg
     1621 cacctggccg atcaccgccg ga
//
LOCUS       DASYLX010000413         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3310756,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000413 DASYLX010000000
VERSION     DASYLX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310756"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGP27_10160"
     CDS             <1..664
                     /locus_tag="VGP27_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851713.1"
                     /translation="VRPADIYLLDEPLSHLDAKLRAAMRAELKQLSAMSSTTTIYVTH
                     DYQEALALGDRIAVLREGRLVQIGTPDEIWRRPADTFVARSLGQPEINILDGVVDDGR
                     IRLGDGSLDVPIPAGAPCDRGDRVRVGLRPCDIHVGSGAGSAHLAGTVMLAERLGRNV
                     ELTVKAGGEQVIVLASGREGVDEGSDITMTVADSDVHVFAAGEGDTARLGADDKTLEA
                     AQ"
     gene            661..1803
                     /locus_tag="VGP27_10165"
     CDS             661..1803
                     /locus_tag="VGP27_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851714.1"
                     /translation="MSVTTDAPRKPASADGTAQSLTLADLVKTYASSGRERVTAVKGI
                     DLAIEPGELVALLGPSGCGKTTTLRMIAGLETVTSGSIKIGEREISQLPASKRGIGVG
                     FESYALYPPMSVRDNLLYGLKARKVKGAEQMVASICDRLEMNDLLGLRPAGLSSGQKQ
                     RVALARALVRNPPVLLLDEPLSHLDASARQRVRRELKVLQREFGYTTIVVTHDQVEAL
                     SLADRLAVMDAGVVQQFGTPDEVFDDPANLFVAGFVGEPQINVIRGVAKVSAGKTRVE
                     IGSDAGHLDTVVTSVADGTRVTVGVRPQDCALGGPESQGISTTVAYFEHLLEFGLATS
                     TVAGMEEGIVVQTPAQESYEPEQKVVVTAPPERVYLFDTETGERLR"
     gene            1800..>2913
                     /locus_tag="VGP27_10170"
     CDS             1800..>2913
                     /locus_tag="VGP27_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase family protein"
                     /protein_id="HEV7851715.1"
                     /translation="MTKLFNDPARFTEDMLVGFLDANSRYVVGVPGGVVRAHQTAPGK
                     VAVVIGGGSGHYPAFCGTVGTGFADGAVVGNIFTSPSAEEAASVARAAHSDAGVLLTT
                     GNYAGDVMNFGLAVTQLRSEGIEAHYFAVTDDIASAPKGEETKRRGIAGDFTVFKCAS
                     AAAEEGLDLSSVIRVAEAANAATRTLGVAFDGCTLPGADEPLFTVPDGQMGVGLGIHG
                     EPGVADEAMPTADQLAVTLVDGVLGDKPETDSTRIAVILNGLGRTKYEELFVVWGAVA
                     RLVRERGYEIVEPEVGELVTSLDMAGCSLTVMWLDNELEKYWRAPADTPAYRKGVTGA
                     ASEGARRSDAAAEAAAPKLAVAELADEDGRRGGQLIA"
BASE COUNT          520 a          961 c          987 g          445 t
ORIGIN      
        1 ggtgcggccc gccgacatct acctgctcga cgaaccgctg agccacctcg acgccaagct
       61 ccgcgccgcg atgcgcgccg aactcaaaca gctcagcgca atgtcgagca ctacgaccat
      121 ctacgtgacc cacgactacc aggaggcctt ggcgctcggc gaccgcatcg cggtgctgcg
      181 cgaaggacgg ctcgttcaga tcggcacgcc ggacgagatc tggcggcgac ctgccgacac
      241 cttcgtggca cgatcgctcg gtcagcccga gatcaacatc ctcgacgggg tggtcgacga
      301 cggccgcatc cgcctcgggg acgggtcgct ggacgtaccg atccccgccg gcgcgccgtg
      361 cgatcgcggc gaccgcgtac gagtcggcct gcggccctgc gacatccatg tcgggtccgg
      421 agcgggctcg gcgcacttgg cgggaaccgt gatgctcgcg gagcggctcg gccgcaacgt
      481 cgagctgacc gtgaaggccg gcggcgagca ggtgatcgtg ctcgcctccg gtcgcgaggg
      541 cgtcgacgaa ggcagtgaca tcaccatgac cgtggccgat tccgacgtac acgtgttcgc
      601 cgcaggcgag ggcgacacgg cccggctggg cgcggacgac aagactctgg aggcggcaca
      661 gtgagcgtga cgacagatgc tccgcgcaaa cccgcatcgg ccgatggcac ggcgcaaagt
      721 ctgaccctcg ccgacctggt caagacctac gcgtccagcg gccgtgagcg ggtgacggcg
      781 gtcaagggca tcgaccttgc catcgaaccc ggagagttgg tcgcgctgct cggcccgtcc
      841 ggctgcggaa agaccacaac tctgcgcatg atcgcgggac tcgaaacggt gacgagcggt
      901 tcgatcaaga tcggcgagcg ggagatctcg cagctgcccg cgtcaaagcg cggtatcggg
      961 gtcggcttcg aaagctacgc gctgtacccg ccgatgtcgg tccgcgacaa cctgctgtat
     1021 ggcctcaagg cccgcaaggt caagggcgcc gagcagatgg tcgcctcgat ctgcgaccgg
     1081 ctcgagatga acgacctgct cgggctacgg cccgccggcc tttccagcgg acagaagcag
     1141 cgcgtcgcgc tggcccgcgc cctggtgcgc aacccaccgg tgctcctgct cgacgagccg
     1201 ctgagccacc tcgatgcctc cgcgcgccag cgggtgcgac gcgagttgaa ggtgctgcaa
     1261 cgtgaattcg gctacacgac gatcgtcgtc acccatgatc aggtcgaagc cctgtcgttg
     1321 gccgaccgac tggcggtcat ggatgccggt gtggtgcagc agttcggcac gcccgacgag
     1381 gtgttcgacg acccggccaa cctcttcgtc gcagggttcg tcggcgaacc acagatcaat
     1441 gtgattcgcg gtgtcgcaaa ggtctccgcc ggcaagactc gtgtcgagat cggttccgat
     1501 gcaggtcatc tcgacaccgt cgtcacctcg gtcgccgatg gcacccgcgt caccgtcggt
     1561 gtccggccac aggactgcgc gctgggtggc ccggagagtc aggggatctc gaccactgtc
     1621 gcctacttcg agcatctgct cgaattcggt ctcgcgacaa gcacggtagc gggcatggag
     1681 gagggtatcg tcgtccagac tccggcccag gagagctatg agcccgaaca gaaggtcgtg
     1741 gtgactgccc cgccggaacg cgtttatctg ttcgacaccg aaacagggga gcggttgcga
     1801 tgaccaaact cttcaacgat cccgcccggt tcaccgagga catgctggtc gggttcctcg
     1861 acgccaactc gcgttacgta gtcggtgtgc ccggcggcgt ggtccgcgcg caccagacgg
     1921 cgcccggaaa ggtagcagtc gtcatcggtg gtggctcagg gcactacccg gcattctgcg
     1981 gaaccgtcgg caccggattc gcggacgggg cggtcgtcgg caacatcttc acctcgccat
     2041 cggccgagga ggcggcgtcg gtggcgcgcg ccgcccacag tgacgcaggt gtcctgctga
     2101 ccaccggtaa ctacgccggc gacgtcatga acttcggcct tgccgtcacc cagttgcgca
     2161 gcgagggcat cgaggcccac tacttcgcgg tcaccgacga catcgcaagc gccccgaagg
     2221 gcgaggagac gaaacgacgg ggcatcgcgg gcgatttcac cgtgttcaaa tgtgccagtg
     2281 ccgccgcaga agagggcctt gacctcagca gcgtgatccg ggtggcagaa gctgccaacg
     2341 cggccacccg cacgctcggc gtcgcattcg acggctgcac cctgccgggg gccgatgaac
     2401 cgctgttcac ggttcccgac ggccaaatgg gcgtcggtct cggcatccac ggcgagcccg
     2461 gtgtggccga cgaggcgatg ccgaccgccg accagctggc ggtcacactc gtcgatggtg
     2521 ttctcggcga caaacccgaa accgacagca cgcggatcgc ggtgatactc aacggcctcg
     2581 gcagaaccaa gtacgaagag ctcttcgtgg tctggggagc tgtggcgaga ctcgttcgcg
     2641 agcgcggata cgagatcgta gagcccgaag tgggcgaact ggtgaccagc cttgatatgg
     2701 cgggctgctc gttgacagtg atgtggctcg acaacgaact cgaaaagtat tggcgggcac
     2761 cggcagacac cccggcctac cgcaagggcg tgactggcgc ggcctccgaa ggcgcccgcc
     2821 gctcggacgc ggcagcagaa gcggccgcgc cgaaactcgc cgtcgccgaa ttggccgatg
     2881 aggacggtcg ccgtggtgga cagctgattg cac
//
LOCUS       DASYLX010000414         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2323439,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000414 DASYLX010000000
VERSION     DASYLX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323439"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGP27_10175"
     CDS             <1..399
                     /locus_tag="VGP27_10175"
                     /inference="COORDINATES: protein motif:HMM:NF039590.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7851716.1"
                     /translation="LPPPSGMVGRVGEPKTAPGKAVRLVRGIMHNLGKADPVHHERPQ
                     LNVSTQDSRWFLLCNVDGVTVTTADSRGVVYRQRDRAKMFALLRASLRRQRKLQRRFD
                     AMREAYRDALPMLSGVQQWEAVFLETPAHD"
     gene            392..940
                     /locus_tag="VGP27_10180"
     CDS             392..940
                     /locus_tag="VGP27_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7851717.1"
                     /translation="MTETSPPLRAWTQRGEEAALVAVQSALAGRPGVLAGARALSHFG
                     EHSIGWLAVAAVGALLQPRKRRTWLAAGFGAVAAHAAAVIIKRVVRRERPHHPDIVVN
                     VGTPSRLSFPSAHATSTTAASILLARATGWPLPALLVPPMALSRLVLGVHYPTDVITG
                     VAVGAAVAKTVGHMAARAEGDS"
     gene            979..1893
                     /locus_tag="VGP27_10185"
     CDS             979..1893
                     /locus_tag="VGP27_10185"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEV7851718.1"
                     /translation="MSEQPAPVKGPPRNVVTGLIKAARPRQWVKNLLVLIAPVAALGS
                     DVNYDWRNVAVKVSIAFVAFSLAASSVYLVNDARDVEADRAHPTKRFRPIAAGVVPPW
                     LAYCTSVVLAVAALAVSFLATPNLAGVIGVYIAMQLAYCFGLKHQAVLDICIVSSAYL
                     IRAIAGGVAANIQLSQWFLLVMTFGSLFMVAGKRYAELQLSEKTGAKIRKSLESYTST
                     YLRFVWTLSATAMVVCYGLWAFERDGANASWYAITMIPFTIAILRYAVDVDGGLAGEP
                     EEIALKDRVLQLLAVAWIGTIGAAVLLG"
     gene            1871..>2766
                     /gene="zomB"
                     /locus_tag="VGP27_10190"
     CDS             1871..>2766
                     /gene="zomB"
                     /locus_tag="VGP27_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor control protein ZomB"
                     /protein_id="HEV7851719.1"
                     /translation="MPQFSWARRGPAFPYDITARTSLWLSVLVAGALFGWGAWQRRWI
                     ADDGLIVLRTVRNLLAGNGPVFNKGERVESNTSTVWTYLNYLGGLIGGPTQLEYVTLW
                     LALLLSVAGVVFLMLGAARLYAPSLQGRRALLLPAGVLVYMAVPPARDFATSGLENGL
                     VLAYLGLLWWMMVCWSQALRGHQDSTQRNAVSAVFDGALAFVAGLSVLVRPELALVGG
                     LALVMMLVAARGWRRRVLIVASGGLLPIAYQIFRMGYYGLLFPQTALAKDASGNKWAQ
                     GWVYLTNFDQPYLLWVPALLLAV"
BASE COUNT          398 a          911 c          942 g          515 t
ORIGIN      
        1 ctgccaccgc catcgggaat ggtgggtcgc gtcggagagc cgaaaaccgc gccgggcaag
       61 gcggttcggc ttgttcgcgg aatcatgcac aacctcggca aggctgatcc cgtgcatcac
      121 gagcggccgc agttgaacgt gtccacccag gattcgcgct ggtttctgct gtgcaatgtc
      181 gacggagtga ccgtcaccac cgctgacagc cgtggcgtgg tgtaccgaca gcgagaccgg
      241 gccaagatgt ttgcactcct gcgggcgtcg ctgcgtcgcc agcgaaaact gcagcggcgc
      301 ttcgacgcga tgcgcgaggc gtaccgggac gcgcttccga tgctctccgg tgtccagcag
      361 tgggaggccg tttttctcga gacgcccgcg catgactgag acctccccac cgcttcgcgc
      421 gtggacccag cgcggcgagg aggccgcgtt ggtggccgtc cagtcggcgt tggccggccg
      481 ccccggcgtt ctcgccggtg cgcgcgccct ttcgcacttc ggcgaacaca gcatcggatg
      541 gctcgcggtc gccgctgtcg gtgcgctgtt gcaaccgcgg aagcggcgta cgtggctggc
      601 cgccggattc ggtgcggtcg ccgcgcacgc cgccgcggtc atcatcaagc gggtggtgcg
      661 acgcgaacgc ccgcatcacc cggacatcgt cgtgaacgtc ggcacgccga gcaggctcag
      721 cttcccgtcc gcgcacgcga cctcgaccac cgcggcgtcg atactgttgg cgcgcgccac
      781 ggggtggccg ctgcccgcgt tgctggtacc gccgatggcc ttgtcccggt tggtactcgg
      841 cgtgcactat cccaccgacg tgatcaccgg cgtggcagtc ggcgcggccg tcgcgaagac
      901 ggtgggtcac atggcggcca gggccgaagg agactcatga ccatctcaag tgacagcagt
      961 gcagagagtt cggcgaagat gagtgaacaa ccggcaccgg tcaaaggccc accccgcaat
     1021 gtggtcaccg gcctgatcaa ggcggcgcgg ccgcgccagt gggtgaagaa cctgctcgtg
     1081 ctgatcgccc ccgtcgccgc cctcggcagc gacgtgaact acgactggcg caacgtggcc
     1141 gtcaaggtct ccatcgcgtt cgtggcgttc tccctcgcgg cgtcgtccgt ctacctcgtc
     1201 aacgacgccc gcgacgtcga ggccgaccgc gcacatccga ccaaacggtt ccggcccatc
     1261 gccgcaggcg tggtgccgcc gtggctggcc tactgcacct ctgtggtcct cgcggtggcg
     1321 gccctggcgg tgtcgtttct cgcgacgccg aacctggccg gggtgatcgg cgtctacatc
     1381 gccatgcaac tcgcatactg cttcggcctc aaacaccaag cggtgctcga catctgcatc
     1441 gtctcgtcgg cgtacctgat ccgggcgatc gccggcggcg tggcggcgaa catccagctg
     1501 tcgcaatggt tcctgctggt gatgacgttc ggctcgctgt tcatggtggc gggcaagcgc
     1561 tacgccgaac tgcagctctc cgagaagacg ggggcgaaga tccgcaagtc gctcgagagc
     1621 tacacctcga cctacctccg gttcgtctgg acgttgtcgg cgaccgcgat ggtggtttgt
     1681 tacgggctgt gggccttcga gcgcgacggt gccaacgcgt cctggtacgc gatcacgatg
     1741 attccgttca cgatcgcgat cctgcgttat gccgtcgacg tcgacggcgg gctggccggc
     1801 gaaccggagg agatcgcgtt gaaggaccgg gtgctccagc ttctggccgt cgcgtggatc
     1861 ggaaccatcg gtgccgcagt tctcctgggc tagacgcggg cccgcgtttc cctacgacat
     1921 caccgctcga accagccttt ggctgagcgt gctcgtcgcc ggggcactgt tcggctgggg
     1981 cgcgtggcaa cgccgctgga ttgccgacga cggcctcatt gtgttgcgca ccgtgcgaaa
     2041 tctgctggca ggcaatggtc cggtcttcaa caagggcgag cgcgtcgagt cgaacacgtc
     2101 gacggtgtgg acctacctga actacctcgg tggcctcatc ggggggccca cccagctcga
     2161 gtacgtgacc ctgtggcttg ccctgctgct cagcgtggcg ggcgtcgtct tcctgatgct
     2221 cggcgccgcc cggttgtacg cgccgagcct gcagggccgc agggcactgc ttctgcccgc
     2281 gggcgtgctg gtctacatgg cggttccgcc ggcccgtgac ttcgccacct cagggctcga
     2341 aaacggtttg gtcctggcct atctcggcct gctgtggtgg atgatggtgt gttggtcgca
     2401 agccttgcgc ggccaccagg acagcacaca gcgaaatgcg gtcagcgcgg tgttcgacgg
     2461 cgcgcttgca ttcgtggccg gtctgtcggt gctggtgcga ccggaactcg cgctcgtcgg
     2521 cgggctcgcc ctggtcatga tgctcgtcgc ggcacggggc tggcggcgcc gggtgctgat
     2581 cgtcgcctcg ggcggactgc ttccgatcgc atatcagatc ttccgaatgg gttattacgg
     2641 attgctcttt ccccagacgg ctttggcgaa ggacgcatcg ggcaacaagt gggcgcaggg
     2701 ctgggtttac ctcaccaact tcgaccagcc ctatctgttg tgggtgcccg cgctgctgct
     2761 ggcggt
//
LOCUS       DASYLX010000415         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1883802,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000415 DASYLX010000000
VERSION     DASYLX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883802"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VGP27_10195"
     CDS             complement(<1..627)
                     /locus_tag="VGP27_10195"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851720.1"
                     /translation="MTAHGDKPASTPCSAARLLARGEPVQSAIAMAESLTALPVIGRR
                     LEPLVTLTAIGAWASRMVPRAISELMKVQGNRTESVPEADETPEPVPVLAEVCSAALQ
                     GVIGDLDGIAWPLPTRAAPAFRMMEQRRRYLHKTAVRYGPHESLLLDVWRLPDLPKTP
                     APVLIFVPGGAWVHGSRTFQGSALMSHLVESGWICLAIDYRVSPRNRWP"
     gene            complement(694..873)
                     /locus_tag="VGP27_10200"
     CDS             complement(694..873)
                     /locus_tag="VGP27_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851721.1"
                     /translation="MTIASPAVSHADCAWGEWWDPVATVCRPTGLVLPKDCTPGWWDP
                     TINDCRPPLGSPPPP"
     gene            complement(1073..1156)
                     /locus_tag="VGP27_10205"
     tRNA            complement(1073..1156)
                     /locus_tag="VGP27_10205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1120..1122),aa:Leu,seq:tag)
     gene            1190..1552
                     /locus_tag="VGP27_10210"
     CDS             1190..1552
                     /locus_tag="VGP27_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851722.1"
                     /translation="MPTTRRRRPALIALVIIAAAGCLALAWWQWTRFESTSGSFQNLG
                     YALQWPLFAGFCVYGYYKFVRFEDAPPEQSRHDTVTEIPAGLLPERPNPSTRSDDDPV
                     LREYNAYLADLAKKDREP"
     gene            1549..1887
                     /locus_tag="VGP27_10215"
     CDS             1549..1887
                     /locus_tag="VGP27_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEV7851723.1"
                     /translation="MTDTAPTTPVETIRKALLAYRLMAWTTGIWLIALCYEIYLRYVV
                     KVDNPPTWIGVVHGWVYFVYLLATANLAVRVRWPIAKTIGVLLAGTIPLVGIIVEHFQ
                     TRDIKQKYGV"
     gene            complement(1983..>2398)
                     /locus_tag="VGP27_10220"
     CDS             complement(1983..>2398)
                     /locus_tag="VGP27_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEV7851724.1"
                     /translation="ATGLASVADDSGLEVDALNGMPGVLSARWAGLHGQDAANTALLL
                     AQLRDVPDERRGGGFVSACALVSSAGETVVRGTWRGSIAREAKGDGGFGYDPVFVPEG
                     SSRTAAELTPQEKDAASHRGRALALLVPALKALSG"
BASE COUNT          441 a          829 c          749 g          379 t
ORIGIN      
        1 gggccagcgg ttccgcgggg acacccggta gtcgatcgcg agacaaatcc aaccggactc
       61 gacgagatgc gacatcagcg ccgacccctg gaaggtgcgg ctgccgtgca cccaggcgcc
      121 gccgggaacg aagatcagca caggcgccgg cgtcttcggg agatccggca ggcgccacac
      181 atccagcaaa agactctcgt ggggcccgta gcggacagca gttttgtgca gatagcggcg
      241 gcgctgctcc atcatgcgaa acgccggtgc cgcgcgtgtt ggcagcggcc atgcgatgcc
      301 gtcgagatcc ccgatgacgc cttggagcgc ggcactgcac acctcggcga ggacgggcac
      361 cggttccgga gtttcgtcgg cttcgggcac cgactccgtt cggttgccct ggactttcat
      421 cagctcactt atcgcacggg gcaccatgcg ggatgcccac gcgccgatgg cggtcagggt
      481 gacgagcggt tcgagtcggc ggccgatgac cggtagggca gtaagcgatt cagccatcgc
      541 gatcgccgat tgcaccggtt cgccgcgagc caggagtctg gccgccgaac acggtgtcga
      601 cgcgggttta tcgccgtggg ccgtcattgc gcgaatgtac cgccggatac gctcgatatc
      661 ggtgagattc gggcgagcgt cagaacttca gtctcacggc gggggtggcg accccagcgg
      721 cggccggcag tcgttgattg tcggatccca ccagccgggc gtgcagtcct tcggcagcac
      781 caagccggtc ggacggcaca ctgtggcgac cggatcccac cactcgcccc atgcacagtc
      841 cgcgtggctc acggcgggag atgcaatggt cacgaaggac agcgaagcga acccggctgc
      901 ggcgacggtc gctaaacgac gaactgtatt tcgcatggag ttgttcttgc ccaacggcgg
      961 gcagttcaaa cgcgtcatca tccgaagact tggagctaac gccgagttcc gcaccggggt
     1021 agttgctcgg gccgtttcac gaccttcatg cagaactggg tggagagaag ggtgcgggcg
     1081 gggggactcg aacccccacg tccaaaagga caatggcacc taaagccatc gcgtaagcca
     1141 attccgccac gcccgcagcg tcccaagggt agcgcggtac cgttgaacgg tgcccactac
     1201 acggcgtagg aggccggcgc tgatcgcgct ggtgatcatc gccgccgcag gttgccttgc
     1261 cctggcgtgg tggcagtgga cccgtttcga atcgacgtcg ggcagcttcc agaacctcgg
     1321 ctacgccctg caatggccgc tgttcgcggg gttctgtgtc tacggctact acaagttcgt
     1381 ccgcttcgag gacgcgccgc cagagcagag ccgccacgac accgtcaccg agatcccggc
     1441 cggcctgctg cccgaacgac ccaacccctc aactcgatcc gacgatgacc ccgtattgcg
     1501 ggagtacaac gcctacctcg ccgacctggc caagaaagac agagaaccgt gaccgatacc
     1561 gcccccacaa ctcccgtcga gaccatccgc aaggcgctgc tcgcctatcg cctgatggca
     1621 tggacaaccg gcatctggct gatcgcgctg tgctacgaga tttacctccg atacgtggtc
     1681 aaagtggaca acccaccgac ctggatcggc gtcgtgcacg gatgggtgta cttcgtctac
     1741 ctgctcgcga cggccaacct cgcggtcagg gtgcggtggc cgatcgccaa gaccatcggc
     1801 gtgcttttgg ccggcaccat tccgctggtc ggcatcatcg tcgagcactt ccagaccagg
     1861 gacatcaaac agaagtacgg cgtctaaccc gtcgagtgcg cggctgtgtc cgccgatacc
     1921 ggcgcgtccc gtacgaaacc gcacggccca gggtttcaac acttcagcag ccaggtatct
     1981 cgtcagcccg aaagtgcctt gagcgccggc accaacaacg ccaacgcccg cccccggtgc
     2041 gacgccgcgt ccttctcctg tggtgtcagc tcggccgccg tccgcgacga accctcgggc
     2101 acgaacaccg ggtcgtaacc gaatccaccg tcgcccttgg cctctcgtgc gatgctgccg
     2161 cgccacgtac cgcggaccac cgtctcaccc gccgaggaca ccaacgcgca cgccgacaca
     2221 aacccgccac ctcgccgctc gtcgggcaca tcgcgaagct gggccagcag caatgcggtg
     2281 ttggccgcgt cctgcccgtg caggccggcc catcgcgccg acaacacacc gggcatgccg
     2341 ttcagcgcgt cgacctccag ccccgagtcg tcggccacgc tcgccaaccc ggtggccc
//
LOCUS       DASYLX010000416         6323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3858519,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000416 DASYLX010000000
VERSION     DASYLX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858519"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGP27_10225"
     CDS             <1..268
                     /locus_tag="VGP27_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HEV7851725.1"
                     /translation="LTNDNLEYYQDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDT
                     ATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML"
     gene            312..458
                     /locus_tag="VGP27_10230"
     CDS             312..458
                     /locus_tag="VGP27_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851726.1"
                     /translation="MGRLHDCRTGVVPALAVVVIGAAVEDAADADAVGVRVVADALVA
                     GTST"
     gene            complement(570..1754)
                     /locus_tag="VGP27_10235"
     CDS             complement(570..1754)
                     /locus_tag="VGP27_10235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7851727.1"
                     /translation="MTQRYDLVIAGGGPSGSAAAWQAAQTGAKVLVLDKADFPRDKPC
                     GDGLTARAVSYLQKMGLADEVSTFHRVNRVTVFSPSQWELSFPKRPGMPDHGHTVSRT
                     HLDTVLLKHAESAGAQVRQGSEVTGPEFDANGRVIGVTLKGGEKVHGDAVIAADGAYS
                     PIKRALKIDSEYNGYSAIAIRSEMHANRPDSDSLDIYLKLAFQGDQLPGYGWVFPMGN
                     GVFNIGLGYINSYKNWQSINATQFLGEFLRTLPADWELPPIEELKKNKSVRAWRLPMG
                     FTAWPPWRPGVLFTGDSLGAGKPASGAGISKALESGLAAGECAIAALQNGGPDDFTNY
                     AQRMEAAWGREYKRGRYFHKLAGIPAVANTGIKFIDNAFLRDRILKALYKKEQGPQHK
                     YK"
     gene            1907..2647
                     /locus_tag="VGP27_10240"
     CDS             1907..2647
                     /locus_tag="VGP27_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851728.1"
                     /translation="MRARPRSRSGHEPGVKVDARSERWREHRKKVRAEIVDAAFRAID
                     RLGPVLSLREIAEEAGTAKPKIYRHFTDKSDLFQAIGERMRDMLWSAIIPSIDVEADS
                     VREIVGRGVAHYVELVDQHPNVVRFLLQGRFADQSAAAMATVNKGRDITLAIAEMMSS
                     ELEEMELNPAAFELAAFAIFGTAASATDWWLGADDDSPRRMPSDEFISHMTTIMMGAI
                     NGTAELLGVKIEADQPLHTAVRRQQPVA"
     gene            complement(2644..3462)
                     /locus_tag="VGP27_10245"
     CDS             complement(2644..3462)
                     /locus_tag="VGP27_10245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7851729.1"
                     /translation="MATINIAVETSPAIPKDPWHTVLTEGHGSLVRARRLFRYLPSAP
                     RCKLCNNPFGGPAGRVLSAAGFSPSRKNPNLCSRCCDVLPAGGAEVDIAVLFADVRGS
                     TVLGRRGAAADFAALLNRFYNVATRTLLRHDAVIDKLIGDEVMAFFVRGISGPRYRQQ
                     AVRAGLDLLDAVRDCSDGNPWLDLGVAVNAGVAYVGNVGSSVVDFTALGDTVNVAARM
                     QEHAAGGELLVASGVADTLMATASRRSLNLRGREEPLDAFVLRAGDPREAPGSG"
     gene            3578..5170
                     /locus_tag="VGP27_10250"
     CDS             3578..5170
                     /locus_tag="VGP27_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV7851730.1"
                     /translation="MHPSGTLPGSTKGLRRAELLAAISLAIDLGLGQPMEHMLRSSLI
                     STRIAELMGLDAQRRATVYYANLVGWIGCHADSYELSALFGDDIAFRADTYGVDMTGL
                     PFLRLMMSHVGRGLPAWERGIRAAAFLVTARNQVGKLIHSHCSSASVLSDRMGLDEQV
                     GQALAYIFERWDGRGMPKGAREEEIPIEVHIVHLADVVEVHLRTSGVQQALEVVKSRR
                     GTQFSPAVADVFERNAVAIVDGLLEIDVWTAALAHAPDLHRTLDGDEIDELLRAVADF
                     VDLKCPSSPGYSRGVAELAAGAARHLRKPSEDVTRLYRAGLVHGLGRLGVSNSIWQKR
                     GPLSTAESERLRMYPYLTGRILSRVGGLESVVSVATKHEERIDGSGFPRGLVGAELSS
                     TDRLLAAAVAYHRLLEARPDRPGLDTLGAAHQLREEARAARLDADSVEAVLAAAGHRV
                     SRRTSQSGGLTAREVEVLRLVAQGRSNRQIAAELYIAEKTARNHVERVYSKLGVNNRT
                     QASLAAIDRGLAVLPSDAAAGL"
     gene            5320..>6323
                     /locus_tag="VGP27_10255"
     CDS             5320..>6323
                     /locus_tag="VGP27_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851731.1"
                     /translation="MTENAIDPPTITRSCTRTPRHAREAPEGGRVEAVNVSRCVGARQ
                     ILQELSLAVEPGELIAIAGGSGAGKTTLLEILAGAQPSSSGEVRHDGVVRGAKVGGDA
                     RIGYVPQDDIIHLEMPLRRTLRYAARLRLPAGTSAAEADRVVEDTMCDLDLSDRAEVP
                     VRALSGGQRKRASIAVELLTRPRLFFLDEPTSGLDPSTAAGVMRLLRRLSERGVTVVI
                     TTHEPAGIDTCDRVVFLARDGHLAFIGDPAQARRYFDVQDLTEVYERLADEATPQVWA
                     QRFSASRPMTQPGAGIASPPTAATRDDLDRTGALRQWWLLTRRNADVLLRNRLTLAIL
                     L"
BASE COUNT         1088 a         2041 c         2109 g         1085 t
ORIGIN      
        1 cctgaccaac gacaacctcg agtactacca ggacgcgtac gagatcggct acgagaagat
       61 catcgacacc tacgccgcgg ccacccaaca cgtggaccag gggctgagcc tgacgctgtt
      121 cttcaaggac accgccacca cgcgcgacgt caacaaggcg cagatctatg cctggcgcaa
      181 gggaatcaag accttgtact acatccggct gcgccagatg gcgctggagg gaaccgaggt
      241 cgagggctgc gtctcctgca tgctgtaggc cctcggggcg gacctctgca agcgcggtgc
      301 gcggaataac cgtgggtcga ctccacgact gtcgcaccgg ggtagtccca gcactggccg
      361 tggtcgtcat aggtgccgcc gtcgaagacg cagcagacgc cgatgccgtc ggtgtccggg
      421 tggtcgccga cgcactcgtt gccggcacga gcacgtaggg tgcttgccgc ccgcccccgt
      481 ccgggcctta agggataggc gaacgcgcgg tttcatacgc cgactgcggc gagtcgcgta
      541 cgaaaccgca cacccgcgac gccggtgaac tacttgtact tgtgctgcgg gccctgttcc
      601 ttcttgtaga gcgccttgag aatccggtcg cgtaggaacg cgttgtcgat gaacttgatg
      661 ccggtgttcg cgacggccgg gatgcccgcg agcttgtgga agtagcggcc ccgcttgtac
      721 tcccggcccc aggccgcttc catccgctgc gcatagttgg tgaagtcgtc ggggccgccg
      781 ttctgcagcg ccgcgatcgc gcactcgccc gccgcgaggc ccgactccag cgccttggag
      841 atgcccgcac cggaggccgg cttaccggca ccgagcgagt ccccggtgaa caggacgccg
      901 ggccgccacg gcggccacgc ggtgaagccc atcggcaacc gccacgcgcg cacgctcttg
      961 ttcttcttca gctcctcgat cggcggcagc tcccagtcgg cgggcagcgt ccggaggaac
     1021 tcgccgagga actgcgtggc gttgatcgac tgccagttct tgtagctgtt gatgtagccg
     1081 agtccgatgt tgaacacgcc gttgcccatc ggaaacaccc agccgtagcc gggcagctgg
     1141 tcaccctgga acgccagttt cagatagatg tccagcgagt ccgagtcggg ccggttggcg
     1201 tgcatctccg agcggatcgc gatggccgag tagccgttgt actccgagtc gatcttcaag
     1261 gcccgcttga tgggggagta ggcgccgtcg gcggctatca ccgcgtcgcc gtggaccttc
     1321 tcgccgccct tcagcgtcac gccgatgacc cggccgttgg cgtcgaactc gggtccggtc
     1381 acctcggacc cttgacgcac ctgggcacct gccgattcgg cgtgcttgag cagcacagtg
     1441 tccagatgcg tgcggctgac ggtgtgcccg tgatcgggca tgcccggacg cttcgggaac
     1501 gacagctccc actgactcgg actgaacacc gtcacccggt tgacccggtg gaacgtggac
     1561 acctcgtcgg cgaggcccat cttctgcaga tagctgaccg cgcgggcggt caaaccgtcg
     1621 ccgcagggct tatcccgcgg aaaatcggcc ttgtcgagca cgagcacctt cgcgccggtc
     1681 tgcgccgcct gccacgcggc ggccgacccc gatggccccc caccggcgat gactaggtcg
     1741 tatcgctgag tcatgactct cgtctcgttg tcttcgctgt cgcacagata gtacccaagt
     1801 cgctgcaggt ttctcctggg ccgaagggcc cggtgaggca cctcgatggc gcgttgcggc
     1861 acggtctgag caggtcagag acgtcgggac gggtacagta gtcggaatgc gcgcaaggcc
     1921 gaggtcacgc tctggtcatg aaccgggcgt caaggtcgac gcccgcagtg aacgttggcg
     1981 tgaacatcgc aagaaggtgc gggcggaaat cgtcgatgcc gccttccgtg ccatcgaccg
     2041 cctcgggccc gtgttgtcgc tgcgcgagat cgccgaagag gcaggcacgg ccaaaccgaa
     2101 gatctaccgg cacttcaccg acaagtccga tctgttccag gcgatcggtg aacgcatgcg
     2161 cgacatgctg tggtccgcga tcattccgtc gatcgatgtg gaggccgact cggttcgcga
     2221 gatcgtcggg cgcggcgtcg cgcattacgt cgagttggtc gatcagcacc ccaacgtggt
     2281 gcgcttcctt ctgcagggca gattcgccga ccaatccgcg gccgccatgg cgacggtcaa
     2341 caagggtcgc gacatcacgc tcgcgatcgc cgagatgatg agcagcgagc tggaggagat
     2401 ggagctcaac ccggcagcct tcgagctggc ggcgttcgcg atcttcggca cggcggcctc
     2461 ggcaacggat tggtggttgg gcgccgacga cgacagcccg cgccggatgc ccagcgacga
     2521 gttcatcagc cacatgacca cgatcatgat gggcgcgatc aacggcacgg ccgagctgct
     2581 gggcgtcaag atcgaggccg atcaaccgct tcacaccgcg gtccgcagac aacaacccgt
     2641 ggcctagccg gatcccggcg cttctcgcgg atctcctgcg cgcagtacga acgcgtcgag
     2701 gggttcctca cgcccacgca ggttcaacga gcgccgcgat gcggtcgcca tgagcgtgtc
     2761 ggccactccg gacgcgacga gcaattctcc gcctgcggcg tgttcctgca tccgcgccgc
     2821 gacgttaacc gtgtcgccga gggcagtgaa gtcgactact gaactcccga cattgccgac
     2881 gtaggcgacg cccgcgttaa ctgccacacc caagtcgagc catgggtttc cgtcgctgca
     2941 gtcacggacc gcgtccagca ggtcgagccc cgcccgaacg gcctgctgtc ggtagcgcgg
     3001 accgctgatg cccctgacga aaaaagccat gacttcgtct ccgatgagct tgtcgatcac
     3061 cgcgtcatgg cgaagcaagg tgcgggtggc gacgttgtag aacctgttga gtaaggcggc
     3121 gaagtccgcc gcggcgccgc ggcggcccag aacggtggag ccacgaacat cggcgaacag
     3181 gacggcgatg tcgacctcgg caccacctgc tggaagcaca tcacaacagc ggctgcacag
     3241 attcggattc ttgcgcgacg gactgaagcc ggcagcggac agaacacggc cggcggggcc
     3301 gccgaaaggg ttgttgcaga gcttgcatcg gggggccgac gggagataac ggaacagtcg
     3361 tcgcgcccgc accagtgatc cgtgaccttc ggtcagcacc gtgtgccacg ggtccttcgg
     3421 gatcgccggt gaggtctcga cggcaatgtt gatggtggcc atggagaaat ggtcgacctg
     3481 gtagcgcttc gggcgcatga ggcatcggcc tcaagtagtc cggccgtcgg ccgctcgttt
     3541 gggtcggcat cgccgttttt gggtgcagaa tgagcacgtg cacccgtccg ggaccctgcc
     3601 gggctcgaca aagggcctgc gcagagcgga gttgctggct gcgatatcgc tggcaatcga
     3661 cctcggcctg ggtcagccga tggaacacat gcttcggtcg tcattgatct cgacgcgcat
     3721 cgccgaactc atgggcctcg acgcgcaacg acgggcgacg gtgtactacg ccaatctggt
     3781 gggctggatc ggttgtcacg ccgattcata cgagctttcg gcgctgttcg gtgacgacat
     3841 cgccttccgg gccgacactt acggcgtgga catgaccggg ctgccgttcc tgcgtctgat
     3901 gatgagccac gttgggcgcg gcctgcccgc gtgggagcgt ggcattcgcg cggctgcgtt
     3961 tctggtcacg gcacgtaacc aggtcggcaa gctcattcat tcgcattgca gttctgccag
     4021 tgttctttcc gatcggatgg gtctggacga gcaggtcggg caggcgttgg catacatctt
     4081 cgagcgctgg gacggccgcg ggatgccgaa aggcgctcgc gaagaagaaa tcccgatcga
     4141 agtccacatc gttcatttgg ctgacgtcgt cgaggtgcat ctgcggacga gcggggtgca
     4201 acaggccctc gaggtggtga aaagccggcg cggcacgcag ttcagccctg cggtggccga
     4261 tgtgttcgaa cgcaacgccg tcgccatcgt cgacggactg ctcgaaatag acgtttggac
     4321 cgctgcgttg gcgcatgcac cggatcttca ccgcactctc gacggtgacg agatcgacga
     4381 gttgctgcgg gccgtagcgg atttcgtcga cctcaaatgt ccgagctcgc ccggctactc
     4441 gcgtggcgtc gccgagctgg ccgctggagc cgcccggcac ctgcggaagc cgtcggagga
     4501 cgtcacgcgt ctctaccggg cgggcctcgt gcacggcctc ggcagattag gcgtgtccaa
     4561 cagcatctgg cagaagaggg gaccgctcag tacggccgaa tccgagcggt tgcgcatgta
     4621 cccgtatctc accggacgga tactcagccg ggtcggcggc ctggagtcgg tggtatcggt
     4681 ggcgaccaag cacgaggagc ggatcgacgg atctgggttt ccccgtgggc tcgtcggcgc
     4741 cgagttgtct tcgacggatc gattgcttgc agcggccgtg gcctatcacc gactgctcga
     4801 ggcgcgtccg gaccgccccg gcctcgatac tctcggcgcc gcacatcaac tgcgcgaaga
     4861 agcacgggcg gcacgcctcg acgcggattc cgtcgaggcc gtccttgcgg cggccgggca
     4921 ccgggtatca cggcgcactt cccagtccgg cgggcttacc gcccgggagg tggaggtcct
     4981 ccgactcgtc gcgcagggga gatcgaaccg tcagatcgct gccgaactgt acatcgccga
     5041 gaaaactgcg cgtaaccacg tcgagcgcgt ctactccaag ttgggtgtca acaaccgcac
     5101 gcaggcgagt ctcgctgcga tcgatcgagg cttggcggtg ctgccctccg acgcggcggc
     5161 aggactgtaa cggctttcgg gcgtcgaaac tgccgatccg gccgcaatac cgttgacggc
     5221 gaacgtcatt agttgaggca tccgccccat gtgacagagc gatcagcgga ccgatgatcg
     5281 tggctgacag agcaagtccg ccacgagaag gggtccgcca tgaccgagaa cgcgatcgat
     5341 ccgccgacca tcacacggtc gtgtacgcgc acaccacgcc acgcacgcga agcaccggag
     5401 ggcgggcggg tcgaagcagt caacgtgagc cggtgtgtcg gcgcacggca gattctccag
     5461 gagctgtcgc tggctgtgga acctggtgag ctgattgcca tcgccggtgg cagcggagca
     5521 ggaaaaacca cccttctgga aattctcgct ggagcgcaac cgtcgtcttc gggagaagtt
     5581 cgccacgacg gtgtcgttcg cggcgccaag gtcggcgggg atgcgcgcat cggctacgtc
     5641 ccccaagacg acatcatcca tttggagatg ccgttgcgcc ggacgctgcg ctacgcggcg
     5701 cgattacggc tgcccgcagg gacttcggcg gcggaagcgg accgcgtggt cgaggacacg
     5761 atgtgcgacc tcgatctgag tgaccgcgcc gaggtgcccg tccgcgctct gtcgggcggc
     5821 cagcgcaagc gggccagcat cgctgtcgag cttctgaccc ggccccgcct tttcttcctg
     5881 gacgaaccga cctcgggcct cgatccgtcg acggcagccg gtgtgatgcg cctgctgcgc
     5941 cggctcagcg agcgcggcgt cacggtcgtc atcacgacgc atgagcccgc aggcatcgac
     6001 acgtgtgacc gggtggtctt tctcgcacgt gatggccatc tggccttcat cggcgaccca
     6061 gcgcaggcgc gtcgctactt cgacgtgcag gatctcaccg aggtgtacga gcggcttgcc
     6121 gacgaggcca cgccccaggt gtgggcgcag cggttctcgg ccagtcgtcc gatgacacaa
     6181 ccaggtgcag gcattgcttc gccgcccact gcggccaccc gggacgacct cgatcgcacg
     6241 ggtgcacttc ggcagtggtg gctgttgacc agacgcaatg cggatgtgct cctgcgcaac
     6301 cggctgaccc tggcgatcct gct
//
LOCUS       DASYLX010000417         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2872100,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000417 DASYLX010000000
VERSION     DASYLX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872100"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /locus_tag="VGP27_10260"
     CDS             complement(<1..2630)
                     /locus_tag="VGP27_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid synthase subunit beta
                     domain-containing protein"
                     /protein_id="HEV7851732.1"
                     /translation="MTINEHDRVSTGRDDDSGAPQSSHALVDRLTAGEPFAVAFGGQG
                     SAWLETLEELVSSAGIESDLATLVGEAELLLQPVARELIVVRPIGFKPMQWVRSLAAE
                     ETVPSAKHLTSAAVSVPGVLLTQIAAVRALSRQGMDLTATPPVAVAGHSQGVLAVEAL
                     AAKGAKDVELLAMAQLIGTAGTLVARRRGISVLGDRPPMVSVTNADPERIAELLEEFV
                     QDVRTVLPPVLSIRNGRRSVVITGTPEQLSRFELYCKQIAEKEEAERKNKIRGGAVFS
                     PMFDPVQVEVGFHTPRLSDAIDMVGGWAHAVGLDVELARRMAEAILVRPVDWVNEINE
                     LHEAGARWILDLGPGDILTRLTAPVIRGLGVGIVPAATRGGQRNLFTVGAVPEVAKPW
                     SSYAPTLVRLPDGSVKLSTKFTRLTGRSPILLAGMTPTTVDAKIVAAAANAGHWSELA
                     GGGQVTEEIFDNRIAELTELLEPGRAVQFNSLFLDPYLWKLQVGGKRLVQKARQSGAP
                     IDGVVVSAGIPELEEAVALIDELNDVGISHVVFKPGTVEQIRSVIRIAAEAPTKPVIM
                     HIEGGRAGGHHSWEDLDDLLLATYSELRSRSNITVCVGGGIGTPERSAEYLSGRWAQA
                     YGFPLMPVDGILVGTAAMATLEATTSPSVKRMLVETGGTDHWISAGKAQGGMASSRSQ
                     LGADIHEIDNTASRCGRLLDEVAGDAEAVTARRDEIIAAMANTCKPYFGDIGEMTYLG
                     WLQRYVELAIGDGDSTADTAAPGSPWLADTWRDRFAEMLKRAEARLHPKDFGPIDTLF
                     NDEALLDKPAQAITALLGRYPDADTIKLHPADVPFFVQLCKTLGKPVNFVPVIDKDVR
                     RWWRSDSLWQAHDARYDADQV"
     gene            complement(3168..3905)
                     /locus_tag="VGP27_10265"
     CDS             complement(3168..3905)
                     /locus_tag="VGP27_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEV7851733.1"
                     /translation="MSAHNSVPRSVSRRDLLKYAAAAPAVLAVSSLVHVPPANAEPLG
                     ILLDYAAGVIRAADIRAAGAHGAIRYVSDRRPGGAWMLGKPIELPEARDLYQNGLKIV
                     SCYQYGKQDTADWLGGQNAGVQHAKRGWQLHVAAGGSYGAPIYASIDDDPSYEQYKQQ
                     VAPYLRAWETVLGHQRVGVYANSKTIDWALQDGLGSWFWQHNWGSPKGFVHPAAHLHQ
                     VEIDKQNVGGVGVDLNHVLKPQFGQWD"
     gene            4063..>4295
                     /locus_tag="VGP27_10270"
     CDS             4063..>4295
                     /locus_tag="VGP27_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEV7851734.1"
                     /translation="MADDLGVTAPLILEPFRIAVPDADLADLKTRLAATRWPEQLPGT
                     PWQRGVPVDYLRRLAGYWADGFDWRAQEAELNS"
BASE COUNT          685 a         1423 c         1431 g          756 t
ORIGIN      
        1 acctggtcgg cgtcgtagcg ggcgtcgtgg gcctgccaca gcgagtcgct gcgccaccag
       61 cgccgcacgt ccttgtcgat gacgggcacg aagttgaccg gcttgcccag cgtcttgcac
      121 agctgaacga agaacggcac gtcggccggg tgcagcttga tggtgtcggc gtccgggtag
      181 cggcccagca acgcggtgat cgcctgcgcc ggcttgtcca gcagcgcttc gtcattgaac
      241 agcgtgtcga tcggcccgaa gtccttcgga tgcagccggg cctcggcgcg cttgagcatc
      301 tcggcgaacc ggtcgcgcca ggtgtcggcg agccacgggc tgccgggcgc tgcggtgtcg
      361 gcggtgctgt cgccgtcgcc gatggcaagc tcgacatagc gttgcagcca gccgaggtag
      421 gtcatctcgc cgatgtcgcc gaagtacggc ttgcacgtgt tggccatcgc ggcgatgatc
      481 tcgtcgcggc gcgcggtcac cgcctcggcg tccccggcca cctcgtcgag cagccgtccg
      541 caacgtgacg cggtgttgtc gatctcatgg atgtcggcgc cgagctggct gcggctggaa
      601 gccatgccgc cctgagcttt tccggcactg atccagtggt cggtgccgcc ggtctcgacc
      661 agcatccgct tgaccgacgg tgacgtggtg gcctccagcg tcgccatcgc ggcggtgccg
      721 acgagaatgc cgtcgacggg catcaacggg aagccgtacg cctgggccca acggccggag
      781 aggtattcgg ccgaccgctc cggggtgccg atgccgccgc cgacgcacac ggtgatgttc
      841 gaccgcgacc gcagttcgga gtaggtggcc aacaggaggt cgtcgaggtc ctcccacgag
      901 tggtggccgc ctgcgcggcc gccctcgatg tgcatgatga ccggcttggt cggcgcttcg
      961 gcggcgatcc ggatgaccga ccggatctgt tcgacggtgc ccggcttgaa cacgacgtgg
     1021 ctgatgccga cgtcgttcag ctcgtcgatg agcgcgacgg cctcttcgag ctcggggatg
     1081 cccgcgctga cgacgacgcc gtcgatcggg gcgcccgact ggcgcgcctt ctgcaccagc
     1141 cgcttgccgc cgacctggag cttccacagg tacgggtcga ggaacaggga gttgaactgg
     1201 acggcgcggc ccggctccag cagctctgtg agttcggcga tgcggttgtc gaagatctcc
     1261 tcggtgacct gtccgccgcc tgcgagctcg gaccagtgcc cggcgttcgc cgcggccgcg
     1321 acgatcttgg cgtcgacggt ggtcggggtc atgcccgcca gcaggatcgg cgacctgccg
     1381 gtcagccggg tgaacttggt cgacagcttc accgaaccgt cgggaagccg cacgagggtc
     1441 ggggcgtagc tggaccaggg cttggccacc tcgggtaccg caccgacggt gaacaggttg
     1501 cgctggccgc cgcgggtggc ggcaggcacg atgcccacgc cgaggccgcg gatgaccggc
     1561 gccgtcagcc gggtcaggat gtcgccgggg ccgaggtcga ggatccagcg ggcgcccgcc
     1621 tcgtgcagct cgttgatctc gttgacccag tcgacagggc gcaccaggat ggcctcggcc
     1681 atcctgcggg cgagctcgac gtcgaggccg acggcgtgtg cccagccacc gaccatgtcg
     1741 atggcgtccg acagccgcgg ggtgtggaag ccgacctcga cctgaaccgg gtcgaacatg
     1801 gggctgaaca cagcgccgcc gcggatcttg ttcttacgct cggcttcttc cttctccgcg
     1861 atctgcttgc agtagagctc gaaccgcgac agctgctcgg gcgtcccggt gatgacgacg
     1921 gagcgcctgc cgttgcgaat cgacagcacc ggcggcagca cggtccggac gtcttgtaca
     1981 aactcctcga gaagctcggc gatgcgctcg gggtcggcgt tggtcaccga caccatcggc
     2041 gggcggtccc cgagcacgga gatgccgcgg cggcgggcca ccagcgtgcc cgcggtaccg
     2101 atcagctgcg ccatcgccag cagctccacg tcctttgcgc ccttggccgc gagcgcctcg
     2161 accgccagca cgccctgtga atgacctgca acggccacgg gcggtgttgc ggtcaggtcc
     2221 atgccctgac ggctcagcgc gcgcacagcg gcgatctggg tcagcaacac gccgggcacc
     2281 gaaacggcgg ccgacgtcaa gtgcttggcg ctgggcacgg tttcctcggc cgccagcgag
     2341 cggacccact gcatcggctt gaagccgatc ggccgcacga cgatcagctc acgtgccacg
     2401 ggctgcagca ggagctcggc ctctccgacc agcgtcgcca ggtcggactc gataccggcc
     2461 gaggacacca gttcctccag cgtctccagc caggcgctgc cctggcctcc gaacgccacg
     2521 gcaaagggct cgcccgccgt caaacgatcg acgagggcgt ggctggattg aggcgcgccg
     2581 gaatcatcgt cgcggccggt ggacactctg tcatgttcat tgatcgtcac gtttcgtaat
     2641 ctcctcagtc ctgcttctcg cacgtctcac gccgtacttc gtcgtctctg ggctgtcgtt
     2701 gtctgcgtgt gtggaccggc tcccccagcg gggtcacact cgattcgtcg tcgaatcccc
     2761 acggcttcgg actcgtcccg ccgagcgggc ggtccgacgg ccccttgtgg accgtccctc
     2821 cggacactcc agccgtacta agagtgtcat aagagcaacc acgatcttcc cgccctaaat
     2881 ggttactggc gagttctacg ctcgggtaac cacgagttgg gtaacaccgg cttcagaacc
     2941 ggctgaagtc ttccaagaca aacgtcctgc atgtaagtgg ttacggtcga gtaggcacaa
     3001 cggcgcagat caaagcatga ctgtgaccaa atcgttatgt aaaaattttg cctcgtcgtt
     3061 ggcgtcgggt tagccacttc ggaagcgaca actcaacgag atttgtcttg tcgcgcgcgc
     3121 cagccacctt ccaaagtttg ctggcgttta ttactgatcg gtaaggttta gtcccattgg
     3181 ccgaactgcg gcttgaggac gtggttgaga tccacgccga cgccgccgac gttctgcttg
     3241 tcgatctcga cctgatgcag gtgggcggcg gggtgcacga agcccttcgg ggagccccag
     3301 ttgtgctgcc agaaccagga gccgaggccg tcctggagcg cccagtcgat ggttttggag
     3361 ttggcgtaga cgcccacccg ctgatgaccg agcaccgttt cccaggccct caaatacggc
     3421 gcgacttgct gcttgtactg ctcatatgac gggtcgtcgt cgatcgacgc atagattggt
     3481 gcgccgtacg aaccgccggc cgcgacgtgt aactgccatc cgcgcttggc gtgctggacg
     3541 cctgcgttct ggccgccgag ccagtcggcc gtgtcctgct tgccgtactg gtagcaggac
     3601 acgatcttca ggccgttctg gtagaggtcg cgggcttccg gcagctcgat cggcttgccc
     3661 agcatccagg cgccgccggg cctgcggtcg gagacatagc ggatagctcc gtgtgcgcct
     3721 gcggcgcgaa tatcggcggc cctgatgacg cctgccgcat agtccagcag gattcccagc
     3781 ggctcggcgt tggccggcgg tacatgcacc aacgacgaca cggctaggac cgccggagcc
     3841 gcggccgcgt acttgagcag gtcccgccgg gacaccgacc gcgggaccga attgtgcgcc
     3901 gacacgtgcc acaggatatg tcaataacct catatttcac ttcaacgcca ctggtcacag
     3961 atgcatcaaa atccccagga ttaccgacgc aactgtcgcg aacgttgctt accggtcgaa
     4021 ctttcggcca aatctcgact ggtaaggaac gttcatcgcg ggatggcgga tgatctcggg
     4081 gtgaccgcac cgctgatcct cgagccgttc cggatcgcag tacccgacgc cgatctggct
     4141 gacctcaaga cccgactggc cgcgacgcgc tggcccgagc agttgccggg cacaccgtgg
     4201 cagcgaggcg tgcccgtcga ttacctgagg cggctggccg gctactgggc cgacggcttc
     4261 gactggcgcg cccaggaggc cgagctgaac tccta
//
LOCUS       DASYLX010000418         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_678418,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000418 DASYLX010000000
VERSION     DASYLX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678418"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="VGP27_10275"
     CDS             <1..2533
                     /locus_tag="VGP27_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEV7851735.1"
                     /translation="SDFGTARIAGGYKTVTGFFTGTLSYTAPEVLAGNSPTVAADVYS
                     LGATLYALIAGSPPHERKADEDLIAHYMRITSTPVPDLRPQGIPTDVCAAIEKALSRE
                     PAGRYASAADFGRELQFAQRHNGLTPDQMALSEPSGHPEQTGGTQALPLPESPGATGL
                     PEESSIPAGGSTGSLPPPTPDMFAHTASVSQSSLKWAPVQAADSADPRTTTVKPETKK
                     DRKRILIASAAALAALILVAGGIYIVTSKRDNGGAPSAPSQPAVEAQPTWEPIGDARV
                     ARDGVAATQADGTIWVFGGMDPEGRISGRHEGYDPAIDSWKSGEDLPVPVQHAMSVTW
                     QDTPVVLGGWRTDGTNSKVATDKVWRVVNSRWTELPPLLQPRAAATAAVVGDRLIVTG
                     GVGADGELLNTTEIFDGTSWKLGAPLPTPRQMLGSASDGKLVYALGGANGTSDLATVE
                     TYDAATDTWASLPELPGRRSDFGVASADGRLVVAGGMSQGRVLKSVVALDLATQSWNG
                     LPDLATARHGMAVAGVGRTIYSIGGANGAGDSQITSSAESLKLAPRKPQPAPQWRVLP
                     DAPTERLMMAWAVLGDEIWIAGGMRHGETLSTVESYNTKTEAWQPRPSLPIPLHHATA
                     ANYRGEMVVIGGTSDNLADASNKVFAFRDGKWADLPNLLNARAAAAAAVAGDKLVVVG
                     GQNDKQLVKQTEVFDGDSWTEAPGMPTPREHLAAVSDGVYVYTVGGRNLSADENSAAF
                     QRFDPASGNWESLPDMPTPRGSYGAAYIDGRIVVAGGEEPTRVLATVEMYDIATRKWA
                     TLAPINTPVHGEVVAAAGSTVYCIGGADRPTHESAVATVEALDFT"
     gene            complement(2587..3201)
                     /locus_tag="VGP27_10280"
     CDS             complement(2587..3201)
                     /locus_tag="VGP27_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7851736.1"
                     /translation="MSSHLEVSKPSGRDMIPLSGQRVTLGKASTNDVALDHDDTVSRL
                     HAVFENLGFAWSIRDLGSRNGTFLNGERISAERVLRSKDEVRVGKTKLIFWEVRDSGE
                     GPREEETRAAQPSELPPRLTRREVDVLVVLCRPLVSDDPFPEPASVRRMAGELFVTEA
                     AVKQHLQNLYDKFAIPAEGDRRVRLANEALRRGAVTIAQLRDGS"
     gene            complement(3268..>3739)
                     /locus_tag="VGP27_10285"
     CDS             complement(3268..>3739)
                     /locus_tag="VGP27_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851737.1"
                     /translation="GLMGDFIPVMGSFVTGEPDTAGSAAVGYVWRYIGDAGGLGVAFF
                     VVAFALGIDRWSKVKAVLASVAFAVFPTWAGLMATVALLPRGQEMMFELNPATVTITL
                     LGHLIFGLVLGLAFLKAPRGRCRSDWPWTPVLQTKTVRRFTPAAWRPTHCTPRA"
BASE COUNT          652 a         1267 c         1268 g          552 t
ORIGIN      
        1 gagcgacttc ggcaccgccc gcatcgccgg tgggtacaag accgtgaccg gtttcttcac
       61 cggaaccctg tcctacaccg cgccggaggt gctcgccggg aactcgccga cggtcgcggc
      121 cgacgtgtat tccctcggcg cgaccctcta cgcgctgatc gctggcagtc cgccgcacga
      181 gcgcaaggcc gacgaggatc tcatcgcgca ctacatgcgg atcacgtcga cgccggtgcc
      241 cgacctgcgt ccgcagggga ttccgaccga tgtgtgcgct gcgatcgaga aggctctgtc
      301 gcgggagccg gccggtcgtt acgcgtcggc agcggacttc ggccgcgaac tgcagttcgc
      361 tcagcggcac aacggcctga caccggacca gatggcgttg agcgaaccca gcggtcatcc
      421 ggagcagacg ggcggcacac aggcgctgcc gttgccggag tcccccggtg caaccggcct
      481 gcccgaggaa agttccatcc cggccggcgg atcgaccggt tccttgccac cgccgacgcc
      541 ggacatgttc gcccacaccg cgtcggtcag tcagtccagc ctcaaatggg cgccggtcca
      601 ggctgccgat tccgccgatc cccggacgac gacggtcaaa cccgagacga agaaggatcg
      661 caaaagaatc ctgatcgcgt cggcggccgc actggccgcg ctgatcctgg tggcgggcgg
      721 catctacatc gtcacatcga agcgcgacaa cggcggtgcc ccatctgctc cgagtcaacc
      781 ggccgtcgaa gcgcagccga cctgggagcc gatcggggat gcccgcgtcg cccgcgacgg
      841 tgtggccgcc acccaggccg atggcaccat atgggtcttc ggcggcatgg acccagaggg
      901 tcggataagt ggccgacacg aaggctacga cccggccatc gatagttgga agagcggtga
      961 agacctgccc gttcccgtcc agcatgcgat gtcggtgaca tggcaggaca ccccggtcgt
     1021 actcggcggc tggcgcaccg acggcacgaa ttcgaaggtc gcgaccgaca aagtgtggcg
     1081 cgtcgtgaac agccgctgga ctgagctgcc acctctcctg cagcccaggg cggcagcgac
     1141 ggccgccgtc gtcggcgacc gtttgatcgt caccggtggg gtgggcgccg acggagaact
     1201 gttgaacacc accgagatct tcgacggcac ctcctggaaa ctcggcgcgc ccctgcccac
     1261 cccacggcag atgctcggct cggcgtcaga cggcaagctg gtctacgccc tcggcggcgc
     1321 gaacggaacc tccgatttgg cgacggtgga gacctacgac gcggcgacgg acacgtgggc
     1381 gagcctgccc gaactcccag gccggcgcag cgacttcggg gtggcaagtg cagacggacg
     1441 gctggtggtg gccggcggca tgtcgcaggg ccgggtcctc aagagcgtgg tcgcactcga
     1501 tctcgccacg cagtcgtgga atgggttgcc ggacttggcg actgcgcgcc atggcatggc
     1561 agtggcaggc gtcgggagga ccatctattc gatcggcgga gccaacggcg caggtgattc
     1621 gcagatcacg tcgtcggccg aatcgttgaa gctggcaccg cgcaaaccac agccggcacc
     1681 ccaatggcgg gtgctgccgg acgcgccgac cgagcggttg atgatggcgt gggccgtgct
     1741 gggcgacgag atctggatcg cgggcggtat gcgccacggc gagacgctct cgacggtgga
     1801 gagctacaac accaagacgg aggcgtggca gccgcggcct tcactgccga ttcccctgca
     1861 tcacgcgacg gcggcgaact atcgcggtga aatggtggtg atcggtggta ccagcgacaa
     1921 cctcgcggac gcatcgaaca aggtcttcgc gttccgcgat ggaaagtggg cggaccttcc
     1981 caacctgctg aacgcccggg ccgcggcggc ggccgcggtc gccggcgaca aactcgtggt
     2041 cgtcggcggg cagaacgaca agcaactcgt caaacagacc gaggtgttcg acggagactc
     2101 ctggaccgag gcgccgggca tgccgacccc gcgcgaacac ctggccgcgg tctccgacgg
     2161 cgtctatgtg tacaccgtcg gcgggcggaa tctgtccgcg gacgagaatt cggcggcttt
     2221 ccaacgattc gatcccgcct cgggaaactg ggaaagcctt ccggacatgc cgactccccg
     2281 cgggagctac ggcgccgcgt acatcgacgg ccgcatcgtc gtcgccggag gcgaggaacc
     2341 gacgcgggtg ctggcgacgg tcgagatgta cgacatcgcc acccggaaat gggcgacact
     2401 ggcgcccatc aacactcctg tccacggcga agtggtggct gccgccggct cgaccgtgta
     2461 ctgcatcggc ggcgcggacc gccccaccca cgaaagtgcc gtcgcgaccg tcgaggcgct
     2521 cgacttcacc tagccgggcg acgatcaagc ggctacgagc cgcgttgagg agcccggcca
     2581 tcctgcctag ctgccgtcac gtagctgagc gatcgtcacc gccccgcggc gcagcgcctc
     2641 attggccaga cggacgcggc ggtcgccctc ggcggggatg gcgaacttgt cgtagaggtt
     2701 ctgcagatgc tgtttgacgg ctgcctcggt cacgaacagc tcgccggcca tccggcgcac
     2761 cgaggccggc tcggggaacg ggtcatcgga gacaaggggg cggcacagca ccaccagcac
     2821 gtcgacctcg cgcctggtca gccgcggcgg gagttccgag ggctgggcgg ctctggtctc
     2881 ctcctcgcgc ggtccttcgc cggagtccct cacctcccag aagatcagct tggtcttgcc
     2941 gacgcgcacc tcatccttgg aacgcaggac ccgttctgcg gagatccgtt ctccgttgag
     3001 aaaggtgccg ttgcgactgc ccagatccct gatcgaccag gcgaacccga ggttttcgaa
     3061 gaccgcgtgc agccgtgaga cggtgtcgtc gtggtcgagc gcgacgtcgt tcgtcgaagc
     3121 cttaccgagg gttactcgct ggccgctgag cgggatcatg tcccgccccg acggcttcga
     3181 cacctccaga tgggacgaca tgtcgacctc ctgtccgggt cattctcgcc caaacacgac
     3241 gtctgtggtg tctaacgcgc ccgtcgatca cgcccgcggc gtgcaatggg tgggtcgcca
     3301 cgcggccggg gtgaaccgcc ggaccgtctt ggtttgcagg acgggggtcc acggccagtc
     3361 actgcggcaa cggccgcgcg gtgccttcag gaacgccagc cccagaacca gcccgaagat
     3421 cagatgcccg aggagggtga tggtcacggt ggcggggttc agctcgaaca tcatctcctg
     3481 gccacggggc aggagcgcga ctgtggccat cagcccggcc caggtgggga agacggcgaa
     3541 ggcgaccgag gccaggaccg ccttgacctt cgaccatcgg tcgatgccca gtgcgaacgc
     3601 gacgacgaag aaggcgaccc ccaacccgcc tgcatcaccg atgtagcgcc acacgtagcc
     3661 gaccgccgca ctgcccgcgg tgtccggttc gccggtgacg aacgacccca tcaccggtat
     3721 gaagtcgccc atcaatccg
//
LOCUS       DASYLX010000419         8830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3199945,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000419 DASYLX010000000
VERSION     DASYLX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199945"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGP27_10290"
     CDS             <1..1129
                     /locus_tag="VGP27_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7851738.1"
                     /translation="QVLARALRRADGPITIIAGEQVARDDARAELAQLRSVLRARVAT
                     VPDAKDVAGTPGLGSSSALGVSGVMGHPTVATAIAGSALCLVVGTQLSVTARAGLDDV
                     LAEVPTYSIGSAVPFVACTHVHTEDLRGSLKLLTQELNGDGRPTGMRVPDEVSRTELQ
                     PPPHDGPGVRYREAMAVLDAALPDGVDIVVDAGNIGASAIHYLPARRDGRFVVALGMG
                     GMGYSFGAALGIAFGRNRRTVVIAGDGSFFMHGMEIHTAVQYGLPVTFLLFDNHAHAM
                     CVTREQHFYNDVYSYNRFGPSRLGAGLAAMFPGLSAVDVDTIDDLPTALRAALDVDGP
                     SVIAVECSADEIPPFAAFLGNAAVKKPVIAEESSPHVVASA"
     gene            1110..1781
                     /locus_tag="VGP27_10295"
     CDS             1110..1781
                     /locus_tag="VGP27_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851739.1"
                     /translation="MSLPALDDITVHRGTADPIDGVIRVETSPREKATPIIMEMMRSV
                     YPHDEVFGTYCTVNDYIDCPPDELFDYMADTRCLEEWTYSLRGFAPTEEPGLWVAYDK
                     LGGDKGSPIFTRTVANKEARTVDYHCAWDQGKHLWMIYVMRIIDAQVVFDKPGSVVLW
                     TNCHHPFYDENPYPETAPPQRPVWVGDFWDMFGPGHLLELKNLKAIVEYRRRNGLPVT
                     PAWME"
     gene            1819..2829
                     /locus_tag="VGP27_10300"
     CDS             1819..2829
                     /locus_tag="VGP27_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV7851740.1"
                     /translation="MTVSLLDISTYLPGEPVSAEYYVGFAGTDELRENLMFRAPKFRH
                     HVAKDETAVDMVERAAAGLLERHGQDTVANADVLITHVQLPDMPFYGSGGGIAHRLGM
                     KPNWVLDLHNGGCAAFVLAMKVARSLIDSGQAETALIAVAQNAAGTIFDQDTIRSKAQ
                     SSVPGDGAAVGLLARSDKSPILDIECRTYGEYAGDMTIAVDPARKWWEAGTGEGCIGF
                     TESKITKVLARGNRQVPEVSYAVCDRIGIKPADLDLLVTNQPNRAFLRNWRDALELPK
                     EKHRDTFEQCGNLFAAGIPVNLERAISDGQLKKGDTALMAAFAHAGDFAGAAVIEWGG
                     RE"
     gene            2829..3887
                     /locus_tag="VGP27_10305"
     CDS             2829..3887
                     /locus_tag="VGP27_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7851741.1"
                     /translation="MGTASSGKASAGADLADAVSALPSAVNPLALSLNENPFPPLPGV
                     RSALRESVDVANRYPEFLPERLRALIAGHVGVREEQVVIGAGATGVIMQVLNAVTSPG
                     DRMVMTSPTFDGYPIFAHMARLRSVTVALDEHGHHDLDAMAEAAGQARVVVVCRPHNP
                     TGTVEPAADIELFLQRVPSGTVVVLDEAYVEFLAPEHRLDALSLVERFPNVVILRTFS
                     KAYGLAGLRIGYGFCAPDLARRLWTMQLPFGIGITGLVAVAASYDAESELQQRIRMIA
                     SERRYLRVRLHSMGVYSTDSHANFVYLPANSRPWMQFFDDTGLRIRDYANGSVRITVG
                     NRESTRAVLNALTPFASP"
     gene            3928..4446
                     /locus_tag="VGP27_10310"
     CDS             3928..4446
                     /locus_tag="VGP27_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851742.1"
                     /translation="MDSESPTDPHKRDPIELARVNWERAGWGDVSGGMVAVTSVMRAH
                     QILLARVENALRPYDLSFARYELLRLLAFSRNGALPITKASDRLQVHVTSVTHAIRRL
                     EAHGLVERLPHPTDGRTTLVQITKLGRSTVEDATVTLNKEVFADIGLSDDESRALAAS
                     IETLRRNAGDFS"
     gene            complement(4443..5672)
                     /locus_tag="VGP27_10315"
     CDS             complement(4443..5672)
                     /locus_tag="VGP27_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV7851743.1"
                     /translation="MTPVGGGPLRPNAVRDVVDADRELALLRELIQAASSGPGVEPLA
                     AAAAKMITAATATDVCFVHVLDDSDRSLTLAGATPPFDSEVGKIRLPLGSGVSGWVAS
                     HREPVVITHDKESDPRYMPFQSLRGSDFTSMVSVPMETDPGGLVGVLNVHTVARREFT
                     SGDVELLLVIGRLIAGALHQARLHRQLVARERAHENFVEQVIEAQELERRRLAADIHD
                     GISQRLITLSYRLDAASRVAQTDPAAMAEQLDIARELVDLTLQEARAAISGLRPPVLD
                     DLGLAGGLASLARSIGQIDVKVELAEKRLPDHVELALYRIAQECLQNVVKHAGASTAR
                     LTFAVDDDTARLEIVDDGVGFDTFEHPLGSDEMGGYGLLSMAERAEIVGGRLNIRSRP
                     GAGTAVTATIPLPNVAD"
     gene            complement(5669..6346)
                     /locus_tag="VGP27_10320"
     CDS             complement(5669..6346)
                     /locus_tag="VGP27_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7851744.1"
                     /translation="MASDSPLASRPVRIVLVDDHEMVIEGLKAMLAAFSDRVAVVGQA
                     IGAERALSVVDELDPDIVLSDVRMQGSSGLDLCQTLRERRSDRKVVMLSVYDDEQYLF
                     QALRVGAAGYLLKSISSDELVRQLEFAHRGATAIDPGMAARAADTAARMQRDEFWPGA
                     RQGLTQRESEILSLVVNGLSNRGIAGKLVIGDETVKTHLRSIYRKLGVSDRTGAVATA
                     LREGIFQ"
     gene            6451..7260
                     /locus_tag="VGP27_10325"
     CDS             6451..7260
                     /locus_tag="VGP27_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7851745.1"
                     /translation="MPTTQQKTWRAGRFWWDCASPLGANVDSLRALVFDLDALADVEC
                     EGHRVAYNAAFAAHGMSFEWSVARYRQLLALSDERQRVSAELRKRSVSTESDVLTRLL
                     ADDIYTTKTMVLDEMILDADLTPRPGLVDLVMEAFIAGVAVAVVTGGQRSWAEPLVRQ
                     LIGDGIVETVVTADDVKKPMPDPEAFRTALWELGIPAENALAVTGSASGLRAATSTGL
                     ATAVITGDGAPDIPAAVSVRPDYAGSEPMRIADCHRLHRRWSADHKPADAA"
     gene            complement(7292..8164)
                     /gene="mmsB"
                     /locus_tag="VGP27_10330"
     CDS             complement(7292..8164)
                     /gene="mmsB"
                     /locus_tag="VGP27_10330"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEV7851746.1"
                     /translation="MNIAFLGLGNMGGPMAANLVAAGHTVHGFDPVPALKEAAAAKGV
                     TIVDSGAAAVADADVVISSLPNGGIVRACYDEVLPVAKPGALFIDTSTISVADARDIH
                     TQATERGFAQLDAPVSGGVKGATAGTLAFMVGGEDEAVERARPVLEPLAGKIIHCGGS
                     GNGQSAKLCNNMVLAVQQIAVGEAFVLAEKLGLSAQSLFDVITGATGNCWSVHTNCPV
                     PGPVPTSPANNDFKPGFATALMNKDLGLAMDAVNSTGSSAPLGSHAAEIYAKYNADHA
                     DKDFSAVIEMLRRP"
     gene            complement(8177..>8830)
                     /locus_tag="VGP27_10335"
     CDS             complement(8177..>8830)
                     /locus_tag="VGP27_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851747.1"
                     /translation="ETKPGPRGISAFIVEKGTPGLSFGADEEKMGWHAQPTAQVIFEG
                     ARIPADAMLGGAEGEGTGFSIAMNGLNGGRINIAACSLGGAQAAYDKAAGYLADRQAF
                     GGPLLDEPTIRFTLADMATALETSRTILWRAATALDDNHPDKVELCAMAKRYVTDTCY
                     DVADQALQMHGGYGYLTEYGLEKIVRDLRVHRILEGTNEIMRVVIGRAAAGRARASA"
BASE COUNT         1461 a         2883 c         2957 g         1529 t
ORIGIN      
        1 gcaggtgctc gcacgggcgc tgaggcgcgc cgacggtccg atcacgatca tcgccggtga
       61 gcaggtcgcc cgcgacgatg cacgcgccga actcgcgcag ctgcgttccg tgctgcgcgc
      121 ccgcgtggcc accgtccccg atgcgaagga tgttgcgggc acccccgggc tggggtcgtc
      181 ctcggcactg ggggtgagcg gtgtgatggg acatcccact gtcgcgacgg cgatcgcagg
      241 cagcgcgctg tgcctggtgg tcggcacgca gctgagcgtc accgcacgcg cgggactcga
      301 cgacgtgctg gcggaggtgc cgacgtactc gatcggctcc gcggtgccgt tcgtggcgtg
      361 cacgcacgtc cataccgagg acctgcgtgg gtcgctgaag ctgctgacgc aggaattgaa
      421 cggcgatggc cgtccgaccg gtatgcgtgt gccggacgag gtgtcgagaa ccgagctgca
      481 gccgccaccg cacgacggcc ccggtgtgcg ctaccgcgag gcgatggccg tgctggacgc
      541 cgcgctgccc gatggcgtcg acatcgtggt cgacgcgggg aacatcggcg catcggcgat
      601 ccattacctt cctgctcgca gggatgggcg gttcgtcgtg gctctcggca tgggcggcat
      661 gggatacagc ttcggcgctg cgctgggtat cgccttcggg cgaaaccgcc ggaccgtggt
      721 catcgcgggc gacggctcat tcttcatgca cggtatggag attcacaccg cggtgcagta
      781 cggcctgccc gtcacgttcc tgctcttcga caaccacgcc cacgcgatgt gcgtgacccg
      841 cgaacagcat ttctacaacg atgtgtacag ctacaaccgg ttcggcccga gccggctggg
      901 cgcgggcctg gcggccatgt tccccggtct gtcggcggtc gacgtggaca ccatcgacga
      961 tctgccgacg gcgttgcggg ccgccctcga tgtcgacggg ccgtcggtca tcgccgtcga
     1021 gtgctcggcc gacgaaatcc cgccgttcgc agcctttctc ggcaacgcgg ccgtgaagaa
     1081 acctgttatc gcagaggaga gcagtcccca tgtcgttgcc agcgcttgat gacatcaccg
     1141 tccaccgagg cacagccgac ccgatcgacg gagtgattcg cgtcgagacg tcgccgaggg
     1201 agaaggccac accgatcatc atggagatga tgcggtcggt ctacccgcat gacgaggtct
     1261 tcggaacata ttgcaccgta aacgattaca tcgactgtcc gcccgatgag ctgttcgact
     1321 acatggctga cacccgttgc ctggaggagt ggacgtacag cctgcgcggg ttcgcgccga
     1381 cggaagagcc gggtctgtgg gtggcctacg acaagctcgg cggcgacaag ggaagcccca
     1441 tcttcacccg cacggtggcc aacaaagagg cccgcacggt cgactatcac tgcgcgtggg
     1501 accagggcaa acatctgtgg atgatctacg tgatgcgcat catcgacgcg caggtggttt
     1561 tcgacaagcc cggctccgtc gtgctgtgga cgaactgtca ccacccgttc tacgacgaga
     1621 atccgtatcc cgagactgcg ccgccacagc gtcctgtgtg ggtcggagat ttctgggaca
     1681 tgttcggacc cggacacctg ctggaactga agaacctgaa ggcgatcgtc gaataccggc
     1741 gccgcaacgg cttgccggtc acaccggcct ggatggagtg aggacccgcc atgaccactg
     1801 cgcacgcgag gagcgaaaat gaccgttagc ctgcttgaca tctccacata cctgcccggg
     1861 gaaccggtca gcgccgagta ctacgtcggg ttcgccggaa ccgacgagtt gcgcgagaac
     1921 ctcatgttcc gggcgccgaa gttccgccat cacgtggcca aggacgagac ggccgtcgac
     1981 atggtggagc gggcggccgc cggcctcctc gaacgccacg gccaggacac tgtcgccaac
     2041 gccgatgtat tgatcaccca cgttcagctg ccggacatgc ccttctacgg cagcggtggc
     2101 ggcatcgcgc atcgccttgg catgaagccg aattgggtgc tcgacctgca caacggcggg
     2161 tgtgccgcgt tcgtgctggc gatgaaagtg gctcgctccc tgattgattc ggggcaggcg
     2221 gagacggcgc tgatcgcggt cgcacagaac gcggcgggca ccatattcga ccaggacacc
     2281 atccgctcca aggcgcagtc gtcggtgccc ggcgacggag ccgcagtggg cctgcttgca
     2341 cggtcggaca agtcgccgat tctcgacatc gaatgccgca cctacggcga gtacgccggt
     2401 gacatgacca tcgccgtcga cccggctcgc aaatggtggg aggccggcac cggcgaaggc
     2461 tgcatcgggt tcaccgaaag caagatcacc aaggtgctgg cgcgcggcaa ccgccaggtg
     2521 cccgaggtgt cgtacgcggt ctgcgaccgg atcggcatca agccggcgga tctcgacctt
     2581 ctggtgacca accagccgaa ccgggcgttc ctgcggaact ggcgcgacgc gctggaactg
     2641 ccgaaggaga agcatcgcga cactttcgag caatgcggca atctgttcgc cgcaggtatt
     2701 ccggtcaatc tcgagcgggc catctccgac ggccaactca agaagggcga tacggcgctg
     2761 atggccgcct tcgcgcatgc gggcgacttc gcaggcgccg ccgtgatcga gtggggcggc
     2821 cgggagtgat gggaacagcg agcagcggta aagcatcagc gggggcagat ctggcagacg
     2881 cggtgtccgc gctgccgagc gcggtgaacc ccttggccct gtcgctcaac gagaatcctt
     2941 tcccgccgct gcccggagtg cggtctgcgc tgagagagtc cgtcgatgtg gcgaaccggt
     3001 atccggagtt tctgcccgag cggcttcgcg ctttgatcgc gggccatgtc ggggtccgcg
     3061 aggagcaggt ggtgatcggc gccggggcga ccggagtgat catgcaggtg ttgaacgcag
     3121 tgaccagtcc gggagaccgg atggtgatga cgtcgccgac gttcgacggc tatccgatct
     3181 tcgcccacat ggcccgcctg cgttcggtca ccgtggcgct ggacgagcac ggccaccacg
     3241 acctcgatgc catggctgag gcggccggcc aggcacgggt cgtggtggtg tgccgtccgc
     3301 acaatccgac cggcaccgtc gagcctgccg cggacatcga gttgttcttg cagcgggtgc
     3361 cctcgggcac cgtggtcgtg ctggatgagg cctatgtcga attccttgcc cccgagcatc
     3421 ggctcgatgc actctcgctg gtcgagcggt tccccaatgt ggtcatactg cgcacatttt
     3481 cgaaggccta cgggttggcc ggcctgcgca tcggatacgg cttctgcgca cccgatctgg
     3541 ccaggaggct gtggaccatg cagttgccgt tcggtatcgg catcaccggt ctggtggcgg
     3601 tcgcggcgtc ctacgatgca gaaagtgagc tgcagcaacg gattcggatg atcgcatcgg
     3661 agcgacggta tctacgggtg cggctgcatt cgatgggtgt gtacagcact gacagccatg
     3721 cgaacttcgt gtacctgccc gccaacagtc ggccgtggat gcagttcttc gacgacaccg
     3781 gtctgcggat ccgcgactac gccaatggca gcgtccggat aaccgtcggc aaccgcgagt
     3841 cgaccagggc ggtgctcaac gcattgacac cattcgcgtc gccctgaaat tcgttcacct
     3901 cggccgcggc ctgcggtatg aagagtcgtg gactcagaat cgcccacgga tccgcacaag
     3961 cgggatccca tcgagctagc gcgtgtcaac tgggaacgcg ccggctgggg tgacgtctcg
     4021 ggtgggatgg tcgcggtcac gtcggtgatg cgggcccacc agatcctgct ggcccgcgtc
     4081 gagaacgcgt tgcggccgta tgacctcagc ttcgcacgtt acgagctgct gcggctgctt
     4141 gcgttcagtc gcaacggcgc gctgccgatc accaaggctt ccgaccggct ccaggtgcac
     4201 gtcaccagcg tgacgcatgc gatccgtcgg ctggaagcac acggcctggt ggagcggctg
     4261 ccccacccca ccgacggccg caccacgctg gtgcagatca ccaagctcgg acgttccacc
     4321 gtcgaggacg cgaccgtgac gctcaacaag gaggtcttcg ccgacatcgg cctctccgac
     4381 gacgaatcgc gggcgctggc ggcatcgatc gaaacgctac ggcgcaacgc cggcgacttc
     4441 tcctagtccg cgacgttggg cagcgggatc gtcgccgtga cggccgtccc cgcgccgggc
     4501 cgcgatctga tgttgagcct gccgccgacg atctcggcgc gttcggccat cgacagcaag
     4561 ccgtagccgc ccatctcgtc gctgcccagc gggtgctcga atgtgtcgaa gccgaccccg
     4621 tcgtcgacga tctccagtcg cgcggtgtcg tcgtcgaccg cgaacgtcag ccgcgccgtc
     4681 gacgcaccgg cgtgtttgac gacgttttgc aggcattcct gcgcgatgcg atacagcgcg
     4741 agttcgacgt ggtcgggcaa ccgcttctcg gcgagttcga ccttgacgtc gatctgccct
     4801 atcgagcgcg ccaggctggc cagcccaccc gccaggccga ggtcgtcgag caccggcggc
     4861 cgcaggccgc tgatcgccgc gcgcgcttcc tgcagtgtca gatcgacgag ttcccttgcg
     4921 atgtccagct gttcagccat cgccgcaggg tcggtttgcg cgacgcggga tgccgcgtcg
     4981 agccgatagg acagggtgat cagccgctgc gagatgccat cgtggatgtc ggccgccaag
     5041 cggcgacgct ccagctcctg ggcctcgatg acctgctcga cgaaattctc gtgcgcgcgt
     5101 tcgcgagcga ccagttgccg gtgcaggcgc gcctggtgca gcgctccggc gatcagccga
     5161 ccgatcacca acagcaattc cacatcgccg gaggtgaact cgcgccgcgc gacggtgtgc
     5221 acgttgagca caccgaccag accgccggga tcggtttcca tcggcaccga caccatcgac
     5281 gtgaaatctg aaccgcgaag cgactggaac ggcatgtacc gcggatcgga ttccttgtcg
     5341 tgggtgatga cgaccggctc gcgatggctg gccacccagc cggagacacc cgaaccgagc
     5401 ggaagccgga tcttgccgac ctcgctgtcg aatggcggcg tcgcgccggc aagggtcagc
     5461 gaccggtcgc tgtcgtcgag gacgtgcaca aagcagacgt cggtggcggt ggccgcggtg
     5521 atcatcttgg cggctgccgc ggcgagcggt tcgacgccgg ggccgctgga ggccgcctgg
     5581 atcagttcac gcaacagcgc gagctcgcgg tcggcgtcga cgacatcgcg aaccgcgttc
     5641 ggtcgcaacg ggcctccacc aactggagtc attggaagat cccctcgcgc agtgcggtgg
     5701 cgacggcgcc ggtgcggtca ctgacgccga gcttgcggta gatcgaccgc agatgcgtct
     5761 tcactgtctc gtcgccgatg accagcttgc ctgcgatgcc ccggttggac aggccgttga
     5821 cgaccagcga aagaatctcg ctctcgcgct gggtgagccc ctgccgtgcc cccggccaga
     5881 attcgtcgcg ctgcatcctg gccgccgtgt cggctgccct ggcggccatg cccggatcga
     5941 tggcggtcgc gccccggtgc gcgaattcga gctggcgcac cagttcgtcg ctgctgatgc
     6001 tcttcaggag gtatcccgct gcgccgaccc gcaaagcctg aaagaggtat tgctcgtcgt
     6061 cgtagaccga caacatgacg acctttcgat ccgatcggcg ctcgcgcagc gtctgacaca
     6121 ggtcaaggcc gctcgaaccc tgcatgcgga cgtcagacag cacgatgtcg ggatcgagtt
     6181 cgtcgaccac gctgagcgct cgctcagcgc cgatggcctg ccccaccacc gccacccggt
     6241 cggagaacgc agcgagcatc gccttgagac cctcgatgac catttcgtgg tcgtccacca
     6301 agacgattcg tactggtctg gaggcgagcg ggctgtcgga cgccatggca ataccttaga
     6361 gccggggtag gaagacgccc ggcaacttcg cctggccaat ccccctaatg ggggaggctg
     6421 gacacctgtg gtctgggcca cgctttggac gtgcctacga cacagcaaaa gacttggcgt
     6481 gcgggtcggt tctggtggga ctgcgcctca ccactcggcg ccaacgtgga ttccctccgt
     6541 gcactggtgt ttgacctcga cgcgctggcc gacgtcgaat gcgaggggca tcgggttgcc
     6601 tataacgccg ccttcgccgc tcacggcatg agcttcgagt ggtccgtcgc gcggtaccgg
     6661 caactgctcg cactctccga cgagcgtcag cgagtctcgg ccgaactgcg caagcgcagc
     6721 gtctccaccg agtccgatgt gctgacccgg ttgctcgccg acgacatcta tacgaccaag
     6781 acgatggtgc tggacgagat gatcctcgac gccgacctga cgccgcggcc gggactggtc
     6841 gacctcgtca tggaggcctt catcgccggc gtcgcggtag ccgtggtcac aggtggccag
     6901 cgaagctggg cggagccgtt ggtgcgtcaa ctgatcggcg acgggatcgt cgagacggtg
     6961 gtgaccgccg acgacgtcaa gaagcccatg ccggatcccg aagctttccg gaccgcactg
     7021 tgggagctcg ggatacccgc ggagaatgcg ctcgccgtca cgggttccgc ttcgggactg
     7081 cgcgccgcta cctccacggg tctggccacc gcggtcatca ccggcgacgg tgcacccgac
     7141 atccccgcgg cggtttcggt gaggccggac tacgcaggca gtgagccgat gcggattgcg
     7201 gactgccacc ggctgcatcg gcgttggtct gcggaccaca agccggccga cgccgcttga
     7261 agcaacttcc gcgcagcact tgagacgtcg tttacggacg acgaagcatc tcgatgacgg
     7321 cgctgaagtc cttgtccgcg tggtcggcgt tgtacttggc gtagatttcg gccgcgtggc
     7381 tgccgagcgg cgcagacgag ccggtcgagt tcaccgcgtc catcgccagg ccgaggtcct
     7441 tattcatcag tgccgtggcg aagcccggct tgaagtcgtt gttggcgggc gatgtcggga
     7501 ccgggccggg gaccgggcag ttggtgtgca cggaccagca gttgccagtc gcaccggtga
     7561 tgacatcgaa gagcgactgc gcggacagcc cgagcttttc ggcaagcacg aaagcctctc
     7621 cgacggcgat ctgctgcacc gcgagcacca tgttgttgca cagtttggcg ctttggccgt
     7681 tgcctgatcc gccgcagtgg attatcttgc ccgccaaagg ttccagtacc gggcgtgcac
     7741 gttcgacggc ctcgtcctcg ccgccgacca tgaaggcgag cgtgcccgcg gtggctccct
     7801 tgacaccacc ggacacgggg gcgtcgagtt gcgcgaatcc tcgctcggtc gcttgggtgt
     7861 ggatgtcgcg ggcgtcggcg accgagatgg tcgatgtgtc gatgaaaagc gccccgggtt
     7921 tcgccacggg tagcacctcg tcgtagcagg ccctgacgat cccgccgttg ggcagcgagc
     7981 tgatgacgac atcggcatcg gccaccgcgg ccgcgccgga gtcgacgatc gtcacgccct
     8041 tggccgctgc cgcttccttg agggcgggca ccggatcgaa gccgtgcacg gtgtgccctg
     8101 cggcgacaag gttggccgcc atcgggccgc ccatgttgcc cagacccaag aacgcgatgt
     8161 tcatccgcgg acctttctat gcagaggcgc gggctcggcc cgccgccgcc cgaccgatca
     8221 ccacacgcat gatttcgttg gttccttcaa ggattcgatg cacccgcaga tcgcggacga
     8281 tcttctcgag tccgtactcg gtgagatagc catagccgcc gtgcatttgc aacgcttgat
     8341 cggcgacgtc gtaacaggtg tcggtgacgt accgcttggc catcgcgcac agttccacct
     8401 tgtcggggtg gttgtcgtcc agtgcggtcg ctgcccgcca caagattgtt cgcgacgtct
     8461 ccaatgcggt ggccatgtcg gccagcgtga accggatcgt cggctcgtcc agcagcggac
     8521 caccgaatgc ctgccggtcg gccaggtagc cggctgcctt gtcgtaggcg gcctgggcac
     8581 cgccgagcga gcacgccgcg atgttgattc ggccgccgtt gagtccgttc atcgcgatcg
     8641 agaagccggt gccttcaccc tcggcaccgc cgagcatcgc gtctgccggg atccgggcgc
     8701 cctcgaagat gacctgcgcg gtcggctggg cgtgccagcc catcttctcc tcgtcggcgc
     8761 cgaaactgag cccgggagtg cccttttcga cgatgaacgc cgagatgccg cgtgggcccg
     8821 gcttggtctc
//
LOCUS       DASYLX010000420         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3199990,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000420 DASYLX010000000
VERSION     DASYLX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199990"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGP27_10340"
     CDS             <1..468
                     /locus_tag="VGP27_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7851748.1"
                     /translation="LAAGGIGSGQQIAAALALGAQGAWTGSQWVMVEESEHTPVQHAA
                     YAKASSRDTVRSRSFTGKPARMLRNDWTEAWEDPDNPTPLGMPLQYMVSGMAVAATHK
                     YPNESVDVAFNPVGQVVGQFNKVEKTSVVIERWVQEYLEATNRLDELNEAASV"
     gene            complement(470..1012)
                     /locus_tag="VGP27_10345"
     CDS             complement(470..1012)
                     /locus_tag="VGP27_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851749.1"
                     /translation="MKKSDKVWSLSVTGGALGAFAALTLAMPLAHADPDPGPPPLPGP
                     GDQSITTQAASSDPALPAPPPAEVPHLSSPENLPPGTSDVPVDQGQSRTGSYLRDLWH
                     AVQTQDISGKDAILLLTQRPLDPNAMPTNGLPAGPQAPLPDAPPPDAQAPDAPMPGAP
                     APGPALPGPAPAPAPGAPTP"
     gene            1239..>2044
                     /locus_tag="VGP27_10350"
     CDS             1239..>2044
                     /locus_tag="VGP27_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid C27-monooxygenase"
                     /protein_id="HEV7851750.1"
                     /translation="MASPNLPAGFDFLDPDMYAERLPIDELAHMRKVAPIWWQEQEKG
                     NLAFGDTGCWVVTKHKDVKEVSRRSDVFSSNKKTALPRYRDEADPASLEAGKVVLLNQ
                     DAPHHTHLRKIISRAFTPRAIESLRDELRERAGDIVKRAAAEGSGDFVEQVSCELPLQ
                     AIAGLMGVPHEDRMKLFEWSNQMVGDQDPEYGRNDPTAASVELIMYGMQMAADRTKNP
                     GEDLVTRLVHADVEGHKLTDDEFGFFVILLAVAGNETTRNSITQGMMAFT"
BASE COUNT          371 a          635 c          706 g          332 t
ORIGIN      
        1 ctggccgcgg gcggcatcgg cagcggccag cagatcgccg ccgcactggc gctcggcgcg
       61 cagggcgcgt ggacgggttc gcagtgggtg atggtcgagg agtcagagca cacgcccgtg
      121 cagcacgccg cgtacgcgaa ggcaagcagc cgcgacaccg tccgcagccg gtcgttcacc
      181 ggtaagccgg cccggatgct gcgcaacgac tggaccgagg cctgggagga tccggacaat
      241 ccgacgcccc tcggtatgcc gttgcagtac atggtgtccg gcatggcggt cgcggcgacg
      301 cacaaatatc ccaacgagag cgtcgacgtg gctttcaacc cggtcggcca ggtcgtcggt
      361 cagttcaaca aggtggagaa gacctcggtc gtcatcgagc gctgggtgca ggagtacctc
      421 gaggcgacga accggctcga cgaactcaac gaggccgcca gcgtctgacc tacggcgtcg
      481 gcgcccccgg cgccggtgcg ggcgcgggtc cgggtaacgc cggcccaggc gctggagcgc
      541 cgggcatcgg cgcgtccggt gcctgcgcat caggtggtgg cgcatcgggc agcggtgcct
      601 gcggcccggc gggtagtccg ttcgtcggca tggcgttggg gtccagcggg cgctgagtca
      661 gcagaagaat cgcatccttg ccgctgatgt cctgggtctg aaccgcatgc cagaggtcac
      721 gcagatacga ccccgtccta ctctgcccct ggtcgaccgg aacatcagag gtgcccggag
      781 gcaggttctc ggggctcgag agatgcggaa cctcggcagg gggcggcgcg ggcagcgcgg
      841 gatcgctcga tgcggcctga gtggtgatcg attgatcgcc gggccccggt aacggcggtg
      901 gccctggatc gggatcggcg tgcgcgaggg gcattgcaag cgtcaacgct gcgaacgccc
      961 cgagcgcgcc accggtcacg gacagcgacc agaccttgtc ggatttcttc accgatgccc
     1021 ccttcgcttt gtcacggtgc tgttgggtgc ttccgccttg gtacaccgat tgttacacct
     1081 gtgcaatctg tgaccaaggg gtcatttatt gcgaagcggc cagaacacgt ttcacttgag
     1141 agtttctccg tagtttcgag aacctcttca cctggtccga tctgtggtgt agcaatgcca
     1201 tacgggcact agacaacgtc gtatgaggag tccaatcgat ggcatctccg aacctcccgg
     1261 cagggttcga cttccttgac ccagacatgt acgcagaacg gctgccgatc gacgaactgg
     1321 cgcacatgcg caaggtggct ccgatctggt ggcaggaaca ggagaagggc aacctggcat
     1381 tcggcgacac cggctgctgg gtggtgacca agcacaagga cgtcaaggag gtgtcccggc
     1441 gcagcgacgt gttctccagc aataagaaga ccgcgttgcc gcggtaccgc gacgaggctg
     1501 atccggcgtc gctggaggcc ggcaaggtcg tgcttctcaa ccaggacgcc ccgcaccaca
     1561 cccacctgcg caagatcatc tcgcgcgcgt tcaccccgcg ggccatcgag tcacttcgcg
     1621 acgagctccg tgaacgggcg ggcgacatcg tcaagcgggc ggcggccgag ggatccggtg
     1681 atttcgtcga gcaggtgtcg tgcgagcttc cgctgcaggc gatcgccggg ttgatgggtg
     1741 tgccgcacga agaccgcatg aagctgttcg aatggtcgaa ccagatggtc ggcgaccagg
     1801 atccggagta cggcagaaac gacccgacgg cagcatcggt cgaactgatc atgtacggca
     1861 tgcagatggc ggccgaccgg acgaagaacc ccggcgagga cctcgtcacc aggctggtgc
     1921 atgccgatgt cgagggccac aagctcaccg acgacgagtt cggcttcttc gtgatcctgc
     1981 tggcggtggc gggcaacgag accacccgca actcgatcac ccagggcatg atggcgttca
     2041 ccga
//
LOCUS       DASYLX010000421         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_239206,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000421 DASYLX010000000
VERSION     DASYLX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239206"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VGP27_10355"
     CDS             complement(<1..585)
                     /locus_tag="VGP27_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEV7851751.1"
                     /translation="MCVLGLGLIGGSVMRAAADAGREVFGYNRSIEGVRAATVDGFDA
                     GENIEKVLKRAAKTGALIVVAVPVPALSIVLGHVRALAPDCPLTDVTSVKVAVLNQVR
                     SVGLLANFVGGHPMAGTAQSGWAAGDSGLFVGAPWVVSVDDHVDPKVWAMVMDLALDC
                     EAIVVPARSDEHDAAAAAISHLPHLMAEALAATAG"
     gene            695..1210
                     /locus_tag="VGP27_10360"
     CDS             695..1210
                     /locus_tag="VGP27_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEV7851752.1"
                     /translation="MGPQRAPAKDADAPDGFGVAVVREDGKWRCSPMRRAALTSLAAA
                     ETELRELRSAGAVFGLLDVDDEFFVIVRPAPAGTRLLLSDATAALDYDIAAEVLEKLD
                     ADIGPDDLDEAEPFEEGDLGLLSDVGLPEAVLGVIISEYDLYADEQLGRIAREMGFAE
                     ELSAVLDRLDR"
     gene            1207..1662
                     /locus_tag="VGP27_10365"
     CDS             1207..1662
                     /locus_tag="VGP27_10365"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV7851753.1"
                     /translation="MTDDDLIRAALDAAGGAGPGDVPVGAVVFGPDGTELARAANARE
                     ELGDPTAHAEILAMRAAAAVFGDGWRLEGTTLAVTVEPCTMCAGALVMARVSRLVFGA
                     WEPKTGAVGSLWDVVRDRRLTHRPAVVGGVLAEECAAPLEVFFARQRLE"
     gene            1692..1782
                     /locus_tag="VGP27_10370"
     tRNA            1692..1782
                     /locus_tag="VGP27_10370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1726..1728,aa:Ser,seq:cga)
     gene            1867..2457
                     /locus_tag="VGP27_10375"
     CDS             1867..2457
                     /locus_tag="VGP27_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7851754.1"
                     /translation="MFPATDAAEAALTVANRFYSPALLNHCLRSYLWGANYAAAHSID
                     FDDELYYVSALLHDIGLTEPFDSHRLAFENAGGDLAWVFSVAAGWPVDRAARAMEIIV
                     LHMRDDVPAAVDPESHLLQVATAWDVAGRHAEEFPPDARADVLARYPRLGFGSEFLAC
                     FEDQAKRKPGSAAASSVANDAAERIAANPLDAEALT"
     gene            complement(2476..3921)
                     /locus_tag="VGP27_10380"
     CDS             complement(2476..3921)
                     /locus_tag="VGP27_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEV7851755.1"
                     /translation="MSPFARIASIPVASAAAVGLVFGMVVAPPASAEPCTGAAAEAQP
                     PSAPNAAATPEVPGVSGPPIGHRPSKANDSAPLPNLGRLSRSIFNSFVPQKQRVQQQA
                     GVAPPPAPPAVPDAAGIAAPAPPAPAPGAAPAPAPGPPPGTALVGWVTGPQSPNDTIK
                     RFAITGTDLGIMWDNGDPGNNQVLMAFGDTIGYCSVRGRQWRYNTMFRTADRSLAQTV
                     SIPNGVVNNRYSGSPLWAPALSKQIINSIKFAPSETGIIPTSGISVGTTQYLSFMSIR
                     SWDSPGSWTTNFSAVAVSPDNGEHWGVYPQSVRPASQDATPKVPYVPGGEKFQVGAFL
                     KPGDGYLYSYGTPSGRSGPAFVSRVPQGLVPELSRYEYWDGGNWVPQNPGAAQPVIPA
                     PVGEMSVQYNTYLKQFLALYCNGNNDVVARTAPAPQGPWGPEQMLVSANDFPGGIYAP
                     LLHPWSKGKEIYYNLSLWSDYNVMLMKTVLP"
BASE COUNT          634 a         1317 c         1415 g          673 t
ORIGIN      
        1 acccgcggtc gcggccagag cctcggccat caggtgcggc aggtgggaga tggcggcggc
       61 cgcggcgtcg tgctcgtcgg atctggcggg gacgacgatg gcttcgcagt ccaacgccaa
      121 gtccatcacc atcgcccaga ccttcgggtc gacatggtcg tcgacgctga ccacccacgg
      181 cgcgccgacg aacaggccgc tgtcgcctgc cgcccagccg gattgagcgg tgccggccat
      241 cggatgacca ccgacgaagt tggcgagcag gccgaccgac ctcacctggt tgagcacggc
      301 gaccttgacg ctcgtcacgt ctgtcagcgg gcagtccggc gccagcgcgc gaacgtggcc
      361 cagcacgatc gacagcgcgg gcacgggcac ggcgacgacg atcagcgcgc cggttttcgc
      421 ggcgcgcttc agcaccttct cgatgttctc tcccgcgtcg aacccgtcca cggtggccgc
      481 acggacgcct tcgatcgacc ggttgtagcc gaagacctcc cggcccgcat cggccgccgc
      541 ccgcatcacc gagccgccga tgaggccgag gccaagcaca cagatgggcg gtttagtcac
      601 ggctccaagg gtggcacatg gtcttgttcg ccggactcgc ggctggtgtg gattgctggt
      661 caaagtcgct ggtcggggac tagcgtaagc gcccatggga ccacagcggg cacctgcgaa
      721 ggacgccgat gcgcctgacg gcttcggggt tgccgtcgtc cgtgaggacg gcaagtggcg
      781 ctgctcgccg atgcgtcgcg ctgcgctgac gagcctggcg gcggcggaaa ccgaactacg
      841 ggaactgcgc agtgcagggg ccgttttcgg gctgctggac gtcgacgacg agttcttcgt
      901 gatcgtgcgc cccgcaccgg cagggacccg gctgctgctc tcggatgcca ccgcggcgct
      961 ggactacgac atcgccgccg aggtgctgga gaagctcgac gccgacatcg gtcccgacga
     1021 cctcgacgaa gccgagccgt tcgaggaagg cgatctgggt ttgttgtcgg acgtcggcct
     1081 gcccgaggcg gtactcggcg tgatcatctc ggagtacgac ctctacgccg acgagcaact
     1141 gggccgcatc gcgcgcgaga tgggtttcgc cgaggaactg tcggcggtgc tcgaccgtct
     1201 cgatcggtga ccgacgacga cctcatccgc gccgcactcg atgccgcggg cggcgcaggg
     1261 ccaggcgatg tgccggtggg tgcggtggtc ttcgggcccg acggaaccga gttggcgcgg
     1321 gcggccaacg cgcgcgagga gctcggggat ccgacggcgc atgcggagat cctggccatg
     1381 cgcgcggcgg cggcggtgtt cggcgacggg tggcggctcg agggcaccac gcttgcggtg
     1441 accgtcgaac cgtgcacgat gtgcgccggt gcgttggtga tggcgcgggt gtcccggctg
     1501 gtcttcggcg cgtgggaacc caagacgggc gcggtgggct cgctgtggga cgtggtgcgc
     1561 gaccgcaggc tgacgcaccg gcccgcagtc gtcggcggag tgctggccga ggagtgcgca
     1621 gcgccgctgg aggtgttctt cgcgcggcag cgattggagt agaccgctcg ttgttcggta
     1681 cgctgccaca cggtggcgtg tccgagcggc ctaaggagca cgcctcgaaa gcgtgtgacg
     1741 ggtaaccccc gtccgagggt tcaaatccct ccgccaccgc catctcgatc cccgttgcgc
     1801 cgccacctcg cgcacgggta gatcaaacac attccatttg gcgagcgcgt gacggatggt
     1861 aggacgatgt tccctgcgac tgacgcggcc gaggccgcgc tcacggtagc caaccggttc
     1921 tactcgccag cattgctcaa tcactgcctc cggtcatatc tatggggagc gaattacgcc
     1981 gcagcgcact ccatcgactt cgacgacgag ctgtactacg tctctgcgct gctccatgac
     2041 atcgggctca ccgagccgtt cgacagtcac agattggcgt tcgagaacgc cggaggagat
     2101 ctggcctggg tcttcagcgt ggcggccggg tggccggtcg accgggcggc cagagccatg
     2161 gagatcatcg tgctgcacat gcgcgacgac gtgcctgcgg ccgtcgaccc cgaatcccac
     2221 cttctgcagg tcgcaaccgc ctgggacgtc gccggacgcc acgcggagga gttcccgcct
     2281 gatgcgcgag cagacgttct cgcgcgctat ccgcggctcg gtttcggctc cgagttcctg
     2341 gcctgcttcg aggaccaggc gaagcgcaag ccgggcagcg ccgccgcctc gtctgttgcg
     2401 aacgatgccg ccgagcggat cgcggccaac ccgctcgatg ccgaggccct cacatgacgc
     2461 ggtgaaccag ttggtctacg gcagcaccgt cttcatcagc atcacgttgt agtccgacca
     2521 cagcgagagg ttgtagtaga tctccttgcc cttggaccac ggatgcagca acggcgcata
     2581 gatgccgccc gggaaatcgt tcgccgagac gagcatctgc tcgggacccc acggaccctg
     2641 cggggccggc gcggtcctcg ccacgacatc gttgttcccg ttgcagtaca gcgccaggaa
     2701 ctgtttgagg taggtgttgt actgcaccga catctcgccg accggggcgg ggatcaccgg
     2761 ttgtgcggca cccgggttct gcggcaccca gtttccgccg tcccaatact cgtatcggct
     2821 caactccggc acgaggccct ggggaacgcg cgacacgaac gccggcccgc tgcggcccga
     2881 cggtgttccg tacgaataca ggtagccgtc gcccggcttc aggaacgcgc cgacctggaa
     2941 tttctctccg cccgggacgt acgggacctt gggtgtggcg tcctgagagg ccggccgcac
     3001 gctctgcgga tacacgcccc agtgctcgcc gttgtcgggc gacaccgcga cagccgagaa
     3061 gttcgtcgtc cacgagccgg ggctgtccca gctcctgatc gacatgaagc tcaggtattg
     3121 cgtggttccg acggaaatcc cggacgtcgg gatgatgccc gtctccgacg gggcgaactt
     3181 gatgctgttg atgatctgct tggacagtgc gggcgcccac agcggcgagc ccgaatagcg
     3241 gttgttcaca acgccattcg ggatgctgac ggtttgggcc aacgagcggt ccgcggtgcg
     3301 gaacatcgtg ttgtaccgcc actgcctgcc acgcacgctg cagtagccga ttgtgtcgcc
     3361 gaacgccatc agcacctggt tgttcccggg atcgccgttg tcccacatga tcccgaggtc
     3421 ggtgcccgtg atcgcgaaac gcttgattgt gtcgttgggg ctctgcgggc cggtgaccca
     3481 gccgacgagc gcggttcccg gcggcggacc cggcgcgggc gcgggtgcag caccgggtgc
     3541 gggagcaggc ggcgcgggcg cagcgattcc ggcagcgtcg ggaactgccg gaggagcggg
     3601 cgggggcgcc accccggcct gctgttgcac ccgctgcttc tggggcacga acgaattgaa
     3661 gatagatcgc gaaagccggc ccaggttggg tagcggcgcg ctgtcgttgg ccttggaggg
     3721 tcgatgcccg atcggcgggc cgctgacacc cggcacctct ggagtcgccg cggcgttcgg
     3781 cgccgaggga ggttgggcct ccgctgcagc tcctgtgcac ggctccgcag aggccggtgg
     3841 tgcgacgacc atcccgaaca cgaggccgac agcggccgcc gacgccaccg gtatcgatgc
     3901 gattcgagca aacggcgaca tgtcacacct ttcaaggagc agtacgtcgc agttgacgtc
     3961 ccgcgctagc tgatgtgacg atagtgattg ttggggctat aggggtcagt gatgtgctga
     4021 aaggtatctt tccgtgacc
//
LOCUS       DASYLX010000422         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2432935,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000422 DASYLX010000000
VERSION     DASYLX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432935"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGP27_10385"
     CDS             <1..732
                     /locus_tag="VGP27_10385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7851756.1"
                     /translation="AYTAALTAQRVDVRERCRFTGLRTAGGRVVGVDTSDGPIDTERV
                     VLTGGPKLAEVGATAGARVPAGGARHQVVVTAPVAAFDVHEVPMVFDLPSGIYWRPGE
                     AGGLLWGMSNPDEAPGVAADFDDTYYQKARDRIEELLPGLKGLGLRRMWAATIDYTPD
                     HLPILGPLLTDDGPVDGTVVASPAGHGMMWGPAVAEVAADLTMTGRCDWLDLTDLGLD
                     RFDADGNSRLAPEPISLPFPEKAAL"
     gene            729..1073
                     /locus_tag="VGP27_10390"
     CDS             729..1073
                     /locus_tag="VGP27_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7851757.1"
                     /translation="MNTIDTALLAKAADAKLEDWGPLSEATGDSMATHGLEMWVDGDK
                     SAGIWQCTPGPSYWVQNENEVIYLLSGRMTVTPEGGESAEVKAGDIAVFPVGWKGTWD
                     IHETVRKVYSIF"
     gene            1181..2617
                     /locus_tag="VGP27_10395"
     CDS             1181..2617
                     /locus_tag="VGP27_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV7851758.1"
                     /translation="MAREISRQTFLRGAAGALAAGTVFGSVRAAADPIATGWESLSSV
                     IGNPVLLPGNGQFATAKQVFNTIYSGSTPAAIVTPTSAADVQKAMAFAAANKLKVAPR
                     SGGHSYIGASTANGAMVFDLRQLAGGTNYDTASGRVTATPATSLWDLHQTLATAGRGI
                     PTGTCPTVGAAGHALGGGLGAQSRHAGLLSDALTSASVVLPGGQAVTASAAENPDLFW
                     ALRGGGGGNFGVTTSLTFSTFPTSDTDVVNLHYPPEAFAQVLVGWQNWLRTADRSNWA
                     LADSTTDTTGTHCRIMATCPAGAGNAVTSAITSAVGVQPTGTDSHTFNYLDLVKYLAV
                     DNLNPAPVGYVGGSDVFPIVDAGAAQGIAAAVSAFPSGAGRMLAIMHALDGALATVAP
                     GATAFPWRGQSALVQWYVEPAGSPAAAVSWLNTAHQAVQRYSVGGYVNYVEANQPPSR
                     YFGPNLSKLSAVRQKYDPGRVMFSGLNF"
     gene            2669..>2839
                     /locus_tag="VGP27_10400"
     CDS             2669..>2839
                     /locus_tag="VGP27_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851759.1"
                     /translation="MSSTAASFAAGVRPDERLEVLFDELAELTGQRNAIDARIVEIVA
                     EVDRDELCGATGA"
BASE COUNT          461 a          945 c          962 g          471 t
ORIGIN      
        1 gcctacaccg cggcgctgac ggcccagcgg gtcgacgtcc gcgagcgctg ccggttcacg
       61 ggactgcgca ccgcgggcgg ccgggtggtc ggcgtcgaca cgtccgacgg cccgatcgac
      121 accgaacgcg tcgtgctcac cggcggcccc aagctcgccg aagtcggtgc gacggcgggt
      181 gcccgagtgc ccgcgggcgg tgcccgccac caggtcgtgg tgaccgcgcc ggtcgcggcg
      241 ttcgacgtgc acgaggtgcc gatggtgttc gaccttccgt ccggaatcta ttggcggcca
      301 ggcgaagccg gaggtctgct ctggggcatg agcaatcccg acgaggcgcc gggagtcgcg
      361 gccgactttg acgacaccta ctaccagaag gcccgcgacc ggatcgagga actgcttccc
      421 ggcctgaagg gactcggcct gcgcaggatg tgggcggcga cgatcgacta cacccccgac
      481 catctgccga tcctcggccc gctgctgacc gacgacggcc cggtcgacgg cacggtggtc
      541 gccagccccg cggggcacgg catgatgtgg ggccccgcgg tcgccgaggt ggccgccgac
      601 ctgacgatga ccggccggtg cgactggctc gacctgaccg atctcgggct cgaccgcttc
      661 gacgccgacg gcaacagccg gcttgcgccc gaaccgattt cacttccctt cccagagaaa
      721 gcagcactgt gaacactatc gacaccgcac tactcgccaa ggccgcagac gcaaaactcg
      781 aggactgggg accgctgtcc gaagcgaccg gtgattcgat ggcgacacac gggctcgaaa
      841 tgtgggttga cggcgacaag tcggcaggta tctggcaatg cacaccggga ccctcttact
      901 gggtgcagaa cgaaaacgag gtcatctacc tgctgtccgg ccggatgacc gtgacgcccg
      961 aaggcggcga gtccgcggaa gtgaaggccg gcgatatcgc ggtgttcccg gtggggtgga
     1021 agggcacgtg ggacatccac gagacggttc gaaaggtcta ctccatcttt tgatctcgta
     1081 tcgattcggt caccgtcgcg catcatgttg cgcgccgccg cggttttagt ggtcccgtag
     1141 tcgacacttt ccgatgtgac cgaatcgcgg gagctcgacg ttggcgcgtg agatctcccg
     1201 ccagacgttc ctgcggggtg ccgcaggagc attggccgca ggcacggttt tcggctccgt
     1261 gcgggccgcc gccgatccga tcgccaccgg ctgggaaagt ctgtccagcg tgatcggcaa
     1321 cccggttctg cttccgggca acggccaatt cgccacggcg aagcaggttt tcaacaccat
     1381 ctacagcggc tccacgccgg cggccatcgt cacccccaca tcggcggcgg atgtgcagaa
     1441 agcgatggcg ttcgctgccg ccaacaagct caaggtcgct ccgcgcagcg gtgggcattc
     1501 ctacataggc gcgtcaacgg cgaacggcgc gatggtgttc gacctgcgtc agctggccgg
     1561 cggaaccaat tacgacaccg ccagcgggcg ggtcacggcg acgcccgcga ccagcttgtg
     1621 ggatctgcac caaacgctgg ccacggcagg caggggcatc ccgaccggca cctgcccgac
     1681 ggtcggtgct gccgggcacg cgctcggcgg cggactgggc gcccagtccc ggcatgcggg
     1741 tctgctttcc gatgcgctga cgtcggcatc ggtggtattg ccagggggcc aggcggtcac
     1801 ggcgtctgcc gccgagaacc ctgacctgtt ctgggcattg cgcggcggcg gcgggggcaa
     1861 cttcggggtg acgacctcgc tgaccttttc cacgttcccc acctcggaca ccgacgtcgt
     1921 gaacctccat tacccgccgg aagcgttcgc gcaggttctg gtcggttggc agaactggtt
     1981 acggaccgcc gaccggagca actgggctct tgcggacagc accaccgaca cgacgggtac
     2041 gcactgccgc atcatggcga cctgcccggc cggcgccggt aacgccgtga cgagtgccat
     2101 cacctcggcc gtcggagtac aaccgaccgg gaccgactct cacaccttca actatttgga
     2161 tctggtgaag tatctagccg tcgacaatct caacccggcg ccggtcggat acgtcggcgg
     2221 atccgatgtc ttcccgatcg tcgacgcggg cgcagcacag ggcatcgccg cggcggtcag
     2281 cgcctttccc tccggggcgg gccgcatgct ggcgatcatg cacgcccttg acggcgctct
     2341 ggccactgtg gcgccggggg ccaccgcatt tccgtggcgc gggcagtccg cgctggtgca
     2401 gtggtacgtc gaaccagccg gttccccggc ggccgccgtc agctggctca acacggcaca
     2461 tcaagcggtt caacggtatt cggtcggcgg ctatgtgaac tatgtcgagg ccaaccagcc
     2521 gccgtcgcgg tacttcgggc ccaacctatc caagctgagc gcggtacggc agaaatacga
     2581 cccgggccgg gtcatgttct ccgggctgaa tttctagcgc aggcttcggc cgtccggttt
     2641 tgtcgtacct gggtgcgatg atggtgtcat gtcttccact gcagcatctt tcgccgcggg
     2701 ggtacgcccc gacgagcggc ttgaggtgtt gttcgatgag ttggctgagt tgaccggtca
     2761 gcgcaacgcg atcgacgcgc gcatcgtgga gatcgtcgcc gaggtggatc gcgacgaact
     2821 gtgtggcgcc acgggcgcg
//
LOCUS       DASYLX010000423         7238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1006532,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000423 DASYLX010000000
VERSION     DASYLX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006532"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..817
                     /locus_tag="VGP27_10405"
                     /pseudo
     CDS             252..817
                     /locus_tag="VGP27_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162888.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
     gene            1021..1734
                     /locus_tag="VGP27_10410"
     CDS             1021..1734
                     /locus_tag="VGP27_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV7851760.1"
                     /translation="MDLSTSHASTQQLTAEPVIATGPRPAVKYTVEAIGTFFLVFTVG
                     AAVGSHSPLAPLAIGAALMVMVYAGGHLSGGHYNPAVTLAALIRHRIGARDAVAYWLS
                     QIGAGLIAALLVRTIIDPTQNAATATLTLNGPTLLAAFVVELLFTFALCYVVLNVATS
                     KDHPTNSFYGLAIGFTVLAGAVAVGGISGGAFNPAVTVAGAVMGIFAWPTLWVYLVAQ
                     LIAGAAAGSTFLALNPDDK"
     gene            1865..2437
                     /locus_tag="VGP27_10415"
     CDS             1865..2437
                     /locus_tag="VGP27_10415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7851761.1"
                     /translation="MSIRILALVGSLRSDSDNRRLAEAAVKLAPSGISVDIFDGLADV
                     VLYNEDIDQPGVVAAADRLRAEVHSADALLLVTPEYNGTMSAALKNAIDWTSRPFGVG
                     VISGKPVAVIGASHGQYGGVWAHDDARKAARIAGADVLDDLKLSVPAAHIRFASVHPV
                     DDEEIAVTMPEILTSLAAAVQSRDGRGSGE"
     gene            complement(2469..2987)
                     /locus_tag="VGP27_10420"
                     /pseudo
     CDS             complement(2469..2987)
                     /locus_tag="VGP27_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            2979..3485
                     /locus_tag="VGP27_10425"
     CDS             2979..3485
                     /locus_tag="VGP27_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851762.1"
                     /translation="MAYEEADTLTRQARRTRLTGTQLRELHGHAWEAAFPAELDVDWT
                     TLTPVRRREMYLLAEALGLTDLADQLTEVIYACTEPEPPPEARYLRGVRVAIVGDHSE
                     VVELRKYAESYGAKLAVNITKTVQWMATATPDANDSRHNTARRLGIPIISPSRDALYS
                     MRQSKRRR"
     gene            4064..4849
                     /locus_tag="VGP27_10430"
     CDS             4064..4849
                     /locus_tag="VGP27_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851763.1"
                     /translation="MNLLRTVTAGALLFLLVACGGSSGQSSDASAPGKSSVGSTAKPS
                     GPVAPLPFNAGGLLAGTAAPNFSGGEQGKVSVVQVGALDEHMSGSAKLPFAFRNNTSE
                     GVSHVDWSGTARSGGAIVATGSSQGTVPTQVKPGEVGLAFIYFDGTAKLPPADAEYEF
                     TVTTSPADQDGYNTAPLKVTEANSSGDAIVGAAVNQTGESVEGPFSVDAYCFDGDKVL
                     SEHGDFAEQDGPISADGQVTFSASLYGANCPTFVVGAGGYFSE"
     gene            complement(5302..5454)
                     /locus_tag="VGP27_10435"
     CDS             complement(5302..5454)
                     /locus_tag="VGP27_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851764.1"
                     /translation="MFAAAAGLPVYGRVGPGGEYVGVRAGVELKPKRGRSRYYVGVSA
                     GRRVSY"
     gene            6248..6442
                     /locus_tag="VGP27_10440"
     CDS             6248..6442
                     /locus_tag="VGP27_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7851765.1"
                     /translation="MHDLRHTYASLARRAGADLRLLQKTMGHASITVTAHIYADLYDD
                     ELDDVASALDALDDTRGDLP"
     gene            complement(6553..6624)
                     /locus_tag="VGP27_10445"
     tRNA            complement(6553..6624)
                     /locus_tag="VGP27_10445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6590..6592),aa:Val,seq:cac)
     gene            6985..7057
                     /locus_tag="VGP27_10450"
     tRNA            6985..7057
                     /locus_tag="VGP27_10450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7018..7020,aa:Gly,seq:gcc)
     gene            7085..7158
                     /locus_tag="VGP27_10455"
     tRNA            7085..7158
                     /locus_tag="VGP27_10455"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7117..7119,aa:Cys,seq:gca)
     gene            7164..7238
                     /locus_tag="VGP27_10460"
     tRNA            7164..7238
                     /locus_tag="VGP27_10460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7196..7198,aa:Val,seq:gac)
BASE COUNT         1323 a         2348 c         2272 g         1295 t
ORIGIN      
        1 ctctggcggt gaaattccag cccgaatgta ctaccgattc ttaagttgaa cctgaacgtc
       61 ctgccgggtg cagccgacga attggcaagt gcggtaaggc ttagctggct agcgggacgg
      121 tccagggtcg ggttgggtgt cgtaaatggc tgacggtacg caattccgcc ccggccggat
      181 taccgggcgg cacgggcgac gccgctcgaa accgcgattc gcgcggcgcc gtcgaacccc
      241 acagcgcggg gttgtccagt cggcctagct ggcttcgggc ggcggtgctg ggcgcgaacg
      301 acggaatcgt gtctgtcgcg ggccaccacg gcgcgtggcg ccgaattcac cgctgcgccg
      361 gcgggcctgg tggccggcgc ggtgtcgatg gcgctccggc gaacatgtgt cggtgtcgag
      421 tcagcgagaa gtcgctgctc gcccgggacc ggcgagagtt gcggtagatg ccggctgcgg
      481 agttggacga actggcggcg atatacgagg ccaagggcgt ctcttcgcgg accgcggcgc
      541 aagtcgccgc cgagttgacc gcccatgacg cgctggccgc ccatgtcgac gccgaatcac
      601 ggcttgaccg agacgattta gccaaccctg caggcggcgg ccgcgtctgc agcatcgttc
      661 acggtcggag cactgttacc cctgctcgct atgttgttgc caccagacgt ttcggcttcc
      721 cgtcaccttc gtcgccgtgc tgatcgcgtt gggcctggcg ggtgcgttca gcgcacgtat
      781 cggagggagc cgcgtcggcc gcgccatgcc gcgggtgggt gatcggtggt cgcgacgagt
      841 atcaccgagc cgcggtggtc gtccgctgtc tgctacaggc gttaaccgtt cacgagatcc
      901 ccgctctcgg ttctagtctc gttaccgcca ccgggtaaac cagaagcggt ggtagcggta
      961 aggatagtcg gccgttagct gcgacgacta atgctgcgcg gagtacgact gtggaaagcc
     1021 atggacctca gcacatctca cgcatcgact caacaactca ccgccgaacc cgtcatcgca
     1081 accggccccc gaccggcggt gaagtacacg gtcgaggcga tcggaacatt ttttctggta
     1141 ttcacggtcg gcgccgccgt aggcagtcac agcccactgg ccccattagc catcggcgcg
     1201 gcgctgatgg tgatggtcta cgccggaggc cacctgtcag gcggccacta caaccccgcg
     1261 gtgaccctgg ccgcgctgat acgccaccgg atcggtgcgc gtgatgcggt ggcgtactgg
     1321 ctgagccaga tcggtgccgg gctgatcgcg gcgctgctgg tgcgcactat catcgatccg
     1381 acgcaaaacg ccgccacggc gacactgacc ctgaacggcc caaccctgtt ggcagcgttc
     1441 gtcgttgagc tgcttttcac cttcgcgctg tgctacgtcg tgctgaatgt ggcgaccagt
     1501 aaagaccacc cgaccaactc cttctacggc ttagcgatcg ggttcacggt gctggccggc
     1561 gccgtcgcgg tcggcggcat ctcaggtggc gcgttcaacc ctgcggtcac cgtcgccggt
     1621 gcggtgatgg gaatcttcgc ctggccgacg ctgtgggtgt acctggtggc gcagctcatc
     1681 gccggcgccg ccgccggatc gaccttcctg gccctcaacc ccgacgacaa ataagccgac
     1741 tcagtccagt cgagtcacac cttgtccaca gcgcagttct tgggaccgac gtcgttgtac
     1801 gcattgcgcc atcgttgccg cccagactcc ctctcaccaa gaaatcgatt acgtaaggat
     1861 caacatgagc atccgaattc tggcgctggt gggcagcctt cgctccgact cggacaaccg
     1921 ccgcctcgcc gaggcggccg tcaagctggc acccagcggc atctcggtcg acatcttcga
     1981 cggcctcgcc gatgtcgtcc tgtacaacga agacatcgac cagccggggg tcgtcgcggc
     2041 tgctgacagg ttgcgtgccg aagttcattc ggccgacgcg cttctgctcg tgacgcctga
     2101 atacaacggc acgatgtccg ccgccctcaa gaacgccatc gattggacct cgcgtccgtt
     2161 cggcgtcggc gtcatcagcg ggaagccagt ggctgtcatc ggggcctcac acggtcagta
     2221 cggcggggtg tgggcacacg atgacgcgcg caaggcagca cgcatcgcgg gggcagatgt
     2281 gctcgatgat ctcaagctgt cggtgccagc ggcacacatc cgcttcgcca gcgtccaccc
     2341 cgtcgacgac gaggagatcg ccgtcacgat gccggaaatc ctgacctctc tcgccgccgc
     2401 ggtccaaagt cgagatggcc gaggcagcgg cgaataggcg cgccacgctg ttgatcgctt
     2461 gaattgtgtc agcgggccga tacgcctggc gtcagttcgt tctgcgcagt ctcgagcgca
     2521 gtgttcacta agtcgagcgt gagagtgtcg ggatggctga tgtggatctc gatctggtac
     2581 tcgagttggc cggtcagccc gaagatcggc aggacgacga attccgttgc caataaatct
     2641 gtttcgagag tacttgtaat cgaccgcaga gagacgattg tcatcagctt ggtcagctgc
     2701 cgcgccaccg tggcagtggg ttccagcgac gccaagattc cggccacccg atcgtgcagc
     2761 gacgcatggg cgtcgtcgaa tcgccacgct ccatagccgc gggcgcggac gtcggcgagc
     2821 acttgccggt gctggtcgat gtcggcctgg gcgagccgcg gcgtgacacc tcgcagccag
     2881 cgctctacct cggcatcgtc tcgccacgcc atcgccacca ggccgaacgg cgggtcgatc
     2941 ggaaagcgct gtccgacaac gtgctcacca tcgctgccgt ggcctacgaa gaagccgaca
     3001 ccctcacccg gcaagcgcgt agaacccgcc tcactggaac acaactacgc gaactccacg
     3061 gccacgcctg ggaggccgcc ttccccgcgg agctcgatgt cgactggacg accttgactc
     3121 cggtgcggcg acgggagatg tacctcctcg ccgaagcgct cggactgacc gacttggccg
     3181 accaactcac cgaagtaatc tacgcctgca ctgaacccga gcccccgccc gaagccagat
     3241 atctgcgtgg tgtacgcgtc gcgatcgtcg gagaccactc cgaggtcgtt gagctgcgca
     3301 agtacgccga atcctacggc gccaaactcg cggtcaacat caccaaaaca gtgcaatgga
     3361 tggccactgc cacccccgat gccaacgact cccgtcacaa caccgcccgc aggcttggaa
     3421 tcccaatcat cagcccgagt agggacgcac tctactcgat gaggcagtca aagaggcgga
     3481 gatgaaagcc gatgagcgac aacgccaaat cgacgaatat gctgcgcaac gtaagaagcg
     3541 ggacgctgaa gccgatgcct actggcgacc gacctggcgt catcagcaac tggagcatga
     3601 cccagcgccc gaaagttggt acggctagcc gctgactcaa actccctgcg atcgctaagc
     3661 tttgagccac cggccctgcg tttcgccgga accgcctcgt tccgaatgtg cttgttagag
     3721 gccataggcc gtctcgacgg tcgcaatgca gacgccacga tctcaggcgt tttgcagcgt
     3781 ttcgttcaca aatccgttca caaaatagcg gtaagccacc gaacccactg cctttactac
     3841 gaagccagca gattcaacat cccgtccctc tccccgcagc tcgggtaagt agacggcgct
     3901 agggaccgta cggtctccta tcgcgttccg gtacggcggg ccgagaggtc agtcagcgaa
     3961 gtgaccggtg ggtgtaggcc agcgctacat agcactcgat gatgcgccgg tctgccaggg
     4021 tgcgaacgct gccgtcgcgg gccgggggtg taccttcccc gctgtgaact tgctgcggac
     4081 tgtgactgct ggtgccctgc tgttcctcct cgtcgcctgc ggcgggtcgt caggccagag
     4141 cagtgacgcg tccgccccag ggaagtcgtc agtcgggtct accgccaaac cgagcggacc
     4201 ggtcgctccg ctgccgttca acgcgggtgg tttgctggcg gggaccgccg cgccgaactt
     4261 ctccgggggc gagcagggca aggtgtccgt cgtccaggtc ggcgcactgg acgagcacat
     4321 gagcgggtcg gcgaaacttc cgttcgcgtt ccggaacaac accagtgagg gcgtctcgca
     4381 cgtggactgg tctggcaccg cgcgcagcgg cggcgcgatc gtcgcgacgg gcagcagcca
     4441 aggcactgtc ccgactcagg tgaagccggg tgaggtcggg ctggcgttca tctacttcga
     4501 tggcactgcg aaactcccgc ccgctgatgc tgagtacgag ttcaccgtga cgaccagtcc
     4561 cgccgatcag gacggataca acaccgcacc gctcaaggtc accgaggcca actcgtccgg
     4621 tgacgcgatc gtcggcgctg cggtgaatca gaccggcgag tcggttgagg gtccgttctc
     4681 ggttgatgcc tactgcttcg acggagacaa ggtgctcagc gagcacggcg atttcgccga
     4741 gcaggacggt ccgatctcgg ctgatgggca ggttacgttc tcggccagcc tgtacggcgc
     4801 gaactgcccg accttcgtcg tcggcgccgg cggctatttc tcggaataag cgagcggcgc
     4861 tgtctacact ccgacgcgat gagcgaaccc gacctcatcc gttattaccg cagggcgttt
     4921 gtgcgaaata gtgacgcaat tcagagcctg accacttgct cagcttcggt tcgcctcttt
     4981 gcgtcgagaa tgcggtctgc tccattccgt actgcgaagc cgagttttcg aacatggggc
     5041 acatcggcga catgtggtcc tatgttggtc ctactagttg gaccgcatca ctgtggtctc
     5101 cgccgtgccc tacaacttca ccggcaagaa gctcgaagtc cccatcaaac gcatactcca
     5161 aggcaccacc gcgccgacgg atcagtgacg gggctgtcgc tgctcggtcc gattccgccg
     5221 gattgatcgc cccatgtcag cgcagcgggc tacccatcgc tctggcggtc aacgacgaca
     5281 tgcgcccgcc agccgggttt gtcagtagga gacgcggcga ccggcgctga cgccgacgta
     5341 gtagcgcgac cggccccgct tgggcttgag ttcaacgcca gcgcgcacgc cgacgtactc
     5401 accgccaggg ccgacccgac cgtacaccgg cagcccggcc gcagcggcga acacctctcg
     5461 tgcagccgaa tcgcctcacc taagaggtcg gcaatgatcg cggttcgcca ctcccgttgc
     5521 gcgcgtactg ggtggttctt gtacttgctc aacatctcag ccgactcggt gccagcgacg
     5581 gcatagataa gtgatgccac aacatcggcg acgcccgaga tgcgctcgtg cgccggacat
     5641 cgccaacaca ctttgtaacc gcacacgggc gctgcgcaca ccccgcacgt gcgcatggct
     5701 tcgcgcacta cattcatgag atggacccgc ggctcggatt gccgcacagg catgtgcacc
     5761 gtcgatgcca ccccaatcac gacgggctgc ttccctctcg aaatcccgcc gcaaggacca
     5821 agttcccacc acagcgctac acgtcccata cctacctatc tactgaggag gtcgccaggc
     5881 tggccggcgt ctgcggcgtc cagggcgacg tcgtcacgct cttgaccttc actggtctga
     5941 gatttggcga gctggtcggc ctccgtgtcg aagatgtcga tctgggagca aggcgaattc
     6001 gcgttaggcg ctccatcacc caggtcggcg gcaagctgat cgaaggaaat ccgaaatccg
     6061 cggccggtcg cagggcggtc ccgataccgc aacgcctatc ccaattctca cgcagagaat
     6121 cagcggaaga ttcccgggcc agccggccat cacgtcgccc aacggcgcgc tgttggggct
     6181 ggagaattgg aagcggtcca ctcgatggcg cgaagggatc gtcgagatag gccggcctag
     6241 cttgcgagtg cacgacctgc ggcacaccta tgcgtcgctg gcacggcgag cgggcgccga
     6301 cctgcggctg ctacagaaga cgatgggcca tgcgtcgatc accgtgaccg cgcatatcta
     6361 cgccgatctt tacgacgacg aactcgacga tgtcgcctca gcactcgacg ccctcgacga
     6421 cacccgcggg gaccttccgt aagaaccccg ccgtatgggg cggacatttt ggccggatat
     6481 tggccggatg ccgatgacaa caaagaacgc agtgatgcgc agaaattagc cctgaactgc
     6541 ggatattatg tgtggtcccg actgggatcg aaccagtgac cttccgcgtg tgaggcggac
     6601 gctctccccc tgagctacga gaccggggac gacgtcgaag gttagcacgg agcggcgcag
     6661 gtgagagaaa ctcagccacc ttcgaggtct ggtccagcag acacatcaag atcacgtaac
     6721 gtgcagcttg aaacgcgtcg attcccgctg atggtgctgc gcgcaggtct cgtagtggcc
     6781 gcgagcggac cgggagacct tgccgtcgcc ccggacgctc gagcgaggtc cgacccagga
     6841 gtgcggtagg cctcttggga gatggtctct cgcggacctg cgtcgcgacg tcctgctcag
     6901 cgtgtcgttg aagggatttg tgtggctccg ctgctgtgga ctatcgtcgt ccttcgcgac
     6961 gggcgacgat ctcgttcgtg gcgcgcggat gtagcgcagt tggtagcgca tcaccttgcc
     7021 aaggtgaggg tcgcgggttc gaatcccgtc atccgctcga aggtgcagtc gcatcaaccc
     7081 ccgcggtgga gtggccgagt ggtgaggcaa cggcctgcaa agccgtgcac acgggttcga
     7141 ttcccgtctc cacctccaag caggcgcgat tagctcagcg ggagagcgct tccctgacac
     7201 ggaagaggtc actggttcaa tcccagtatc gcgcacca
//
LOCUS       DASYLX010000424         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2651701,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000424 DASYLX010000000
VERSION     DASYLX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651701"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VGP27_10465"
     CDS             <1..1494
                     /locus_tag="VGP27_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEV7851766.1"
                     /translation="MAASRPKTSRHIEVERKFDVLDSTVSPSFDGLSAVARVQRSPAQ
                     QLEAVYFDTPGHDLAAHRVTLRRRTGGTDAGWHLKLPAGPDTRTEVRAPLDDGLGGAV
                     PEALTDVVAAIVRDRPLGPVARISTHRTVDVLYNPDDSPIAEFSDDKVTASAGDGEPQ
                     QWREWELELSESASTSTDLLDRLANRLLDAGAEPAGHGSKLARVLAGSADDEAETDTQ
                     RDPIHRAVAEQVDQLIEWDRAVRADVYDSVHQMRVTTRKIRSLLQSSEGAFGISDDAW
                     VLDELRQLAAVLGVARDAEVLAERYEKALAELPEDLVRGAVDKRLVDGAKRRYKSGLR
                     RSLMAMRSARYFRLLDALEGLVASEPPSPTGDDDGSATASIDSAYKRVRKAAKNASGA
                     ADDDRDEALHRIRKGAKRLRYTAAATGEAKVSDRAKDIQSLLGDHQDSVVSRTHLSQQ
                     AEAAHAAGEDTFTYGLLYAQEDELARKARDQLDSALKKLDKSVHKAR"
     gene            complement(1502..>1803)
                     /gene="rnpB"
                     /locus_tag="VGP27_10470"
     ncRNA           complement(1502..>1803)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGP27_10470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          318 a          610 c          612 g          263 t
ORIGIN      
        1 atggctgcga gcaggccgaa aacgtcccgg cacatcgagg tcgagcgcaa attcgacgtg
       61 ctcgattcca cggtttcacc gtcgttcgat ggtctttcgg cggtggcgcg ggtgcaacgg
      121 tcaccggcac aacagctcga ggcggtctac ttcgacacgc cgggccacga cctggcggcg
      181 caccgcgtca cgcttcgcag gcggaccggc ggaaccgacg ccggctggca cttgaagctg
      241 cccgcgggcc ccgacacccg caccgaggta cgggctccgc tggacgacgg cctgggtggc
      301 gcagtgccgg aagccctgac ggacgtggtt gcggccatcg ttcgcgatcg gccgctgggt
      361 cccgtcgccc gaatttcgac acaccggacc gtcgatgtgc tctacaaccc cgatgactcc
      421 ccgatcgccg agttcagcga cgacaaggtc actgcgtcgg ccggcgacgg cgagccacag
      481 cagtggcgcg aatgggagct ggaactctcc gaaagcgcat cgacgtcgac agacctgttg
      541 gaccggctgg ccaaccgcct cctggacgcg ggtgcggagc cagccggaca cggttcgaag
      601 ctcgcgcggg tgttggcggg ctcggcggac gacgaggcgg agacggatac ccagcgtgat
      661 cccatccacc gggccgtcgc cgaacaggtc gaccagctga tcgagtggga ccgcgcggtc
      721 cgtgcggacg tctacgactc ggtgcaccag atgcgggtca ccacgcgcaa gatccgcagc
      781 ctgctgcagt cctcggaagg cgcgttcggc atttccgacg atgcgtgggt cctcgacgaa
      841 ctgcgccagc tcgcggccgt cctcggggtg gcccgcgacg ccgaagtcct ggccgagcgc
      901 tacgagaagg cgttggccga actgccggaa gacctggtcc ggggcgcggt cgacaagagg
      961 ctggtggacg gcgccaagcg gcgctacaag tccggcttac ggcggtctct gatggcaatg
     1021 cgttccgcac gctacttccg tctgctcgac gcactcgagg ggctggtcgc gtcggaaccg
     1081 ccgtcgccga ccggggatga cgacgggtcg gcaaccgcca gcatcgattc ggcctacaag
     1141 cgcgtgcgca aggccgccaa gaacgcatcc ggcgcggccg acgatgaccg cgacgaagcg
     1201 ctgcaccgaa tccgcaaggg cgccaagcga cttcgctaca ccgcggccgc aaccggggaa
     1261 gccaaggtct cggatcgggc caaggacatc cagagcctgc tgggtgacca ccaggacagc
     1321 gtcgtcagcc gcacgcatct gagccaacag gccgaagccg cccacgccgc aggggaagac
     1381 accttcacct acggcctgct gtatgcgcag gaagacgagc tggcgcgcaa ggcacgtgac
     1441 cagctcgaca gcgcgctgaa gaagttggac aagtcggtgc acaaggcccg ttagacgggg
     1501 gcgaacgagt tggcctgtaa gccggattct gtccctcgcc gccgcgaatt accacggcgg
     1561 cgaggcggcg accatccatc tgggcacacc gtcaccgggt gcctcaagcg gcctacccgc
     1621 aggctcgggc gagcaaccct cgagcgcctg cgcagccgca caaggtgcgg ccttttggcc
     1681 ttgcttcggg tggggtttgc ctagccatcc cggtcacccg ggatgctggt gcgctcttac
     1741 cgcaccgttt cacccttacc accaaaaggt ggcggtctgt tttctgtggc actttcccgc
     1801 gag
//
LOCUS       DASYLX010000425         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2104477,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000425 DASYLX010000000
VERSION     DASYLX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104477"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VGP27_10475"
     CDS             <1..837
                     /locus_tag="VGP27_10475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7851767.1"
                     /translation="PHCARIQPAQLARGLADTVAQLGVDIYEQSPVTAIDSGRAVTPH
                     GTVTAPIVLRATEGFTARLPGLRRRWLPMNSSMIATEPIPDDRWDDIGWRGRETVGDS
                     AHGFFYAQRTLDNRIAIGGRSVPYRYASRIDKDGRVPERTIAHLTETLRAVLPQIRDV
                     PIAHGWCGVLAVPRDWSAGVALDKATGLGWAGGYVGHGVTATNLAGRTLADLVLEQRT
                     PLTELPWVGHQSPNWEPEPLRWLGVRSMYLAYKAADWHEGRGRSTTSPIAVVADKIAG
                     RP"
     gene            complement(896..1366)
                     /locus_tag="VGP27_10480"
     CDS             complement(896..1366)
                     /locus_tag="VGP27_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851768.1"
                     /translation="MTDRGAASVAADITIDADPTKVYELITDLPTLASLAPESAGMRW
                     RSGHKATPGAVFKGTNRNGFRRWTTTCTVTDADPGRAFAFDVHYLGAPVAHWRYDITA
                     EGKGCRVTEQTWDHRAPWMAKAGGLATGVTDRGTANDGHMKLTLQRLKEKAEAG"
     gene            1478..1855
                     /locus_tag="VGP27_10485"
     CDS             1478..1855
                     /locus_tag="VGP27_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV7851769.1"
                     /translation="MTEKPVREPGADHPITVAPTGKHVTVRVNGEVVADTDSALTLQE
                     STYPAVQYIPIGDVVQTALTSSDTTTYCPYKGDASYYHVTTGSGDTVDDAIWTYVQPY
                     PAVAGIAGHVAFYPDKADISVAA"
     gene            complement(1852..>2519)
                     /locus_tag="VGP27_10490"
     CDS             complement(1852..>2519)
                     /locus_tag="VGP27_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5914 domain-containing protein"
                     /protein_id="HEV7851770.1"
                     /translation="PWHGLRLEGGREFGWKPYPAHDDGVLAWVRLDRIGGEQPTEAPV
                     VPVRPDGASMHAVTRLVGTCEPSDIIANRLDPWHGGWYHPYSFAHLDVLSAPPVDADL
                     PQEQDRFLVPVTFHIGRFGVPVVAEFAAPGPRTIVMRIIDGEGAGSVVETHATPIGPG
                     PDGLPRSAVIEAVIAHSDRPGFGHALRARPLIAPFMRQAAARLWRDDLAYAERRFRVR
                     TRG"
BASE COUNT          392 a          884 c          852 g          391 t
ORIGIN      
        1 ccgcactgtg cccgcatcca acccgcccag ctcgcccgcg ggcttgccga caccgtggcc
       61 caactgggcg tggacatcta cgagcagtca ccggtcaccg cgatcgactc cggcagggcg
      121 gtgacgccgc acggcacagt caccgcaccg atcgtgctgc gggcgaccga gggtttcacc
      181 gccaggctgc cgggactgcg ccgacgctgg ttgccgatga acagttcgat gatcgccacc
      241 gagccgatcc ccgatgatcg gtgggacgac atcggctggc gaggccgcga gaccgtcggc
      301 gacagtgcgc acggattctt ctacgcgcag cgcaccctcg acaaccgcat cgcgatcggc
      361 ggccgcagcg tgccctaccg ctacgcatca cgcatcgaca aggacgggcg cgttcccgaa
      421 cgcacgatcg cgcacctgac cgagacgctg cgcgcggtgc tgccgcagat ccgtgacgtg
      481 ccgatcgcgc atggctggtg tggcgtgctg gcagtgcccc gcgactggtc ggcaggcgtt
      541 gcactcgaca aggccaccgg actgggctgg gccggaggat atgtcggcca cggtgtcacc
      601 gcgacgaatc ttgctggccg caccctcgcc gatttggtgc tcgagcagcg gactcctctc
      661 accgagctgc cgtgggtggg ccaccaatcg ccgaactggg agcccgagcc actgcgctgg
      721 ctgggcgtgc gcagcatgta tctcgcatac aaggcggcgg actggcatga gggtcgtggc
      781 cgatcgacga cctcgccgat cgccgtcgtc gccgacaaga tcgccggccg cccctagccg
      841 gcttgagctg ttcgccgggt tcctcaacgc ggcgcgttgc ctcgccgctt gattgtcacc
      901 cggcttcggc cttctccttg agccgctgca gggtcagctt catgtggccg tcgttcgcgg
      961 tgcctcggtc ggtcacacct gtggcgagac cgcccgcctt cgccatccag ggcgcccggt
     1021 gatcccaggt ctgctccgtg acgcgacatc ctttgccctc ggccgtgatg tcgtaacgcc
     1081 agtgcgcgac cggcgcgccg aggtagtgga catcgaacgc gaacgcgcgg ccgggatcgg
     1141 cgtcggtgac ggtgcacgtc gtcgtccagc gccgaaaacc gttgcggttg gtccccttga
     1201 acaccgctcc gggggtcgcc ttgtggccgc tgcgccacct catccccgcg gactccgggg
     1261 cgagcgacgc cagcgtcggc aggtcggtga tcaactcgta aaccttggtg gggtccgcat
     1321 cgatggtgat gtccgccgcg acggaggcgg ctccgcgatc agtcattcgg cgatgttagc
     1381 ggcggacatc gccggacatc ggcggacgag gaagaaaacg cgcgtctcga caggttgcga
     1441 ctatggagat gggcaatcca ttcgtcagga gacagccatg accgagaaac ccgttcgcga
     1501 gcccggcgcc gaccacccga tcaccgtcgc acccaccggt aagcatgtca ccgtgcgggt
     1561 gaacggcgag gtcgttgccg acaccgacag cgcgctgacc ctgcaggagt cgacgtatcc
     1621 ggccgttcag tacatcccga tcggcgacgt cgtgcagacc gcgctgacgt cgagcgacac
     1681 cacgacgtac tgcccgtaca agggtgacgc cagctactac cacgtgacga ccgggtccgg
     1741 cgataccgtc gacgacgcga tctggacgta cgtgcagccg tacccggcgg tcgccggcat
     1801 cgccggtcat gtcgcgttct atcccgacaa ggccgacatt tcggtcgccg cctaaccgcg
     1861 cgtgcgcacc cggaaacgcc gttcggcgta ggcgaggtcg tcgcgccaca gtctggccgc
     1921 cgcctgccgc atgaagggcg cgatcagcgg tcgtgcgcgc agggcatgcc cgaagccggg
     1981 gcggtcggag tgcgcgatga ccgcctcgat caccgcactg cgcggcagcc cgtcgggtcc
     2041 cggcccgatc ggtgtggcgt gcgtttcgac gacgctgcct gcgccctcgc cgtcgatgat
     2101 gcgcatcacg atggtgcgtg gccccggggc ggcgaactca gcgacgaccg gcaccccgaa
     2161 ccggccgatg tggaacgtca ccggcaccag gaagcggtcc tgctcctgcg gtaggtcggc
     2221 gtccaccggc ggggcggaca acacgtcgag gtgggcgaac gagtatgggt gataccagcc
     2281 gccgtgccac ggatccagcc tgttggcgat gatgtcgctg ggttcacacg tgcccaccag
     2341 ccgggtgacg gcgtgcatgc tcgcgccgtc gggcctgacc ggcaccaccg gagcttcggt
     2401 cggttgctcg ccgccgatgc gatcgagccg gacccacgcc agcaccccgt cgtcgtgcgc
     2461 cgggtacggc ttccagccga attcgcgacc accttcgagg cgcagtccgt gccacgggc
//
LOCUS       DASYLX010000426         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2651839,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000426 DASYLX010000000
VERSION     DASYLX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651839"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VGP27_10495"
     CDS             <1..966
                     /locus_tag="VGP27_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851771.1"
                     /translation="GADYHRVFWLTTAFTVIVIAIVVLAVPARPRSATGSIDWLGAAG
                     LAAGLSAVLLAITQGHSWGWTSPRTLVCATGGILITVGWWFWERRAKQPLVSTKMLSR
                     RPILLTNLATVFVGMGLYFAFLGLTQFVQIPHEAAGYGFGATVLEASVVYLLPGAVTG
                     FLVALISGRFIDRFGARPVLVVAAAAGITGFLFIAFVHSAPWQVIVASILANAYISLG
                     YGALPALVVSEVTAADTGVATGMNAIARTIGSSIAAALVAVLLGRTAGGTSVPMESSF
                     VAIFGGGAVTGAAAMVLIALSRVKTGGVDTVEARYESRAMNHEWG"
     gene            complement(963..1391)
                     /locus_tag="VGP27_10500"
     CDS             complement(963..1391)
                     /locus_tag="VGP27_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851772.1"
                     /translation="MATIDVTVKTDLAPEQAWKLASDLHRFDEWLTIFGGWRSEVPTA
                     IEVGTCVSSCIKVKGFRNIIHWRVTRYEEPRLISMSGAGRGGIRINLTIEILDDPPGS
                     KFKVSAELKGGLLSTRIGQLVAKVLQSEIRKSVTNLAALR"
     gene            1762..2853
                     /locus_tag="VGP27_10505"
     CDS             1762..2853
                     /locus_tag="VGP27_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7851773.1"
                     /translation="MAVNEADFSIASGEFFSLLGPSGCGKTTTLRMIAGFETPTEGAI
                     RLEGRDVSRVPPHKRNVNTVFQHYALFPHMTVWDNVAYGPRSQRIDRAKGKGEVKRRV
                     DELLEIVRLTDFAKRKPAQLSGGQQQRVALARALVNYPSALLLDEPLGALDLKLRQVM
                     QFELKRIQREVGITFVYVTHDQEEALTMSDRIAVMNAGNVDQIGSPTEIYDRPATVFV
                     ASFIGQANLWAGRQTGRANREFVQVEVLGSSLKARAGDTTIEPGGHATLMIRPERVRV
                     SMDAPTGDVATVPATVTDLTFQGPVVRLSLAAADDSPIIAHVGPEQDLPLLRPGDKVH
                     VSWAPEASLVLPAADIPTTEDLEEMLDDS"
BASE COUNT          483 a          961 c          929 g          483 t
ORIGIN      
        1 ggcgccgact atcaccgcgt cttctggttg accaccgcct tcaccgtcat cgtgatcgcc
       61 atcgtggtcc tcgcggtgcc cgcacggccg cgcagcgcaa cggggtcgat cgactggctc
      121 ggcgccgccg gcctggcggc cggactgtcg gcggtcctgc tggcgatcac ccagggtcac
      181 agctggggct ggacgtcacc ccgcacgctg gtgtgcgcga cgggcggcat cctgatcacg
      241 gtcggctggt ggttctggga gcggcgggcc aagcagcccc tggtgtcgac gaagatgctg
      301 agccggcgcc cgatcctgct caccaacctc gccaccgtct tcgtgggcat gggcctgtac
      361 ttcgccttcc tcggcctgac acagttcgtc cagatcccgc acgaggcggc gggatacggc
      421 ttcggcgcaa ccgtcctcga ggccagcgtc gtctacctgc tgccaggcgc cgtcaccggt
      481 ttcctcgtcg cattgatcag cggcaggttc atcgaccgct tcggcgcccg gccggtgctc
      541 gtcgtcgcgg ccgccgcggg catcaccgga ttcctgttca tcgcgttcgt gcactcagcc
      601 ccctggcagg tcatcgtcgc cagcatcctg gccaatgcct acatcagcct cggctacggc
      661 gcgctgcccg cgctggtcgt aagcgaggtg accgctgccg atacgggcgt tgccaccggc
      721 atgaacgcga tcgcgcgcac gatcggaagc tctattgcgg ccgcgctcgt cgcggtcctg
      781 ttgggccgaa ccgccggtgg tacctcggtg ccgatggaga gcagtttcgt cgcgatcttc
      841 ggcggcggcg ccgtgaccgg cgcggcggcg atggtgctga tcgccctgtc ccgggtgaag
      901 acaggtggtg tcgataccgt cgaggcgcgc tacgaatcgc gggccatgaa ccacgaatgg
      961 ggctagcgca atgcggccag gttggtcacc gacttgcgga tctccgactg cagcaccttc
     1021 gcgaccagtt gtccgatcct cgtgctgagc aggccgccct tcagctcggc cgagaccttg
     1081 aacttcgaac ccggcgggtc gtcgagaatc tcgatggtca gattgatgcg gatgccgccg
     1141 cggcccgcac cgctcatcga gatcagcctg ggctcctcgt agcgggtcac ccgccagtgg
     1201 atgatgttgc gaaatccttt gaccttgatg cacgacgaga cgcaggtgcc gacttcgatt
     1261 gcggtaggca cctcgctgcg ccagccgccg aagatggtca accactcgtc gaagcggtgg
     1321 aggtctgacg ccagcttcca ggcttgctcg ggggcgaggt cggtctttac cgtcacgtca
     1381 atcgtcgcca taacgctttc gtaccccgcg caacggggtg acgaaactaa actgacacgt
     1441 ctcgcgtcat ccgctgcgcg aatggctggc cggggtcagc cgggggccac ctgcggccgc
     1501 atccgcgaaa tctgttggcc gatcgtggct cggacgagtg attccattgt aaatatgtaa
     1561 attcgttgcg gaatcgcttg cgaaggacga cggaatccgc gattgtttac cggaaaccta
     1621 cgggggcttt agggaggact cgccctgacc ggcatacaca ccaccgacgc ggaccagtcg
     1681 actggacaag aaggggccgc ccgcaccacg ggcagtccgg tcgtcgagat cgacaatgtc
     1741 acgaagcgct tcaccgacta cgtggccgtc aacgaagccg acttctccat cgcttccggc
     1801 gagttcttct cgctgctggg gccatccggt tgcggcaaga cgaccacact gcggatgatc
     1861 gccgggttcg aaaccccgac agagggcgcc atccggctcg agggccgtga cgtctcgcgg
     1921 gtgccgccac acaaacgcaa cgtcaacacg gtgttccagc actacgcgct gttcccccac
     1981 atgacggtgt gggacaacgt cgcctacggg ccgcgcagcc aaaggataga tagggccaag
     2041 ggaaaaggag aagtgaagcg gcgcgtcgac gagctgctcg agatcgtgcg cctgaccgac
     2101 ttcgcgaagc gcaagcccgc gcagctctcc ggcggacagc aacaacgcgt ggcgctggcc
     2161 cgcgcactgg tgaactatcc cagcgcgctg ctgctggacg agccgctcgg cgccctcgac
     2221 ctcaagctgc ggcaggtcat gcagttcgag ctcaagcgca tccagcgcga ggtcggcatc
     2281 acgttcgtct acgtgaccca cgaccaagag gaagcgctca cgatgagcga ccgaatagcg
     2341 gtcatgaacg cgggcaacgt cgaccagatc ggcagtccga ccgagatcta cgaccggccg
     2401 gcaaccgtat ttgtcgccag tttcatcgga caggccaacc tgtgggcggg tcggcagacc
     2461 ggtcgggcga accgcgagtt cgtccaggtc gaggtgctgg gtagctcgct gaaggccagg
     2521 gcgggcgaca ccaccatcga acccggtggc cacgccacct tgatgattcg ccccgaacgg
     2581 gtccgggtgt cgatggatgc gcccaccggc gatgtcgcga cggtgcccgc cacggtcacc
     2641 gacctcacgt tccagggacc ggtcgtgcgg ctctcgctcg ccgcagccga cgactcgccg
     2701 atcatcgcgc acgtcggacc cgagcaggac ctgccgctac tgcggcccgg cgacaaggtt
     2761 cacgtcagct gggcgcccga ggcgtcgctg gtgcttcccg ccgccgacat tcccaccacc
     2821 gaggacctgg aggagatgct cgacgactcg tgacgg
//
LOCUS       DASYLX010000427         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_787911,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000427 DASYLX010000000
VERSION     DASYLX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787911"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..418
                     /locus_tag="VGP27_10510"
     CDS             8..418
                     /locus_tag="VGP27_10510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV7851774.1"
                     /translation="MRSLTSLLADGPIVLFFYPAAMTPGCTKEACHFRDLAGEFTAVG
                     ASRVGISTDPVAKQAKFSDTQRFDYPLLSDADGSVAGQFGVKRGLLGKLMPVKRTTFV
                     IDTDGTVLDVIASEFSMETHADKALEVLRQRQSA"
     gene            415..1215
                     /locus_tag="VGP27_10515"
     CDS             415..1215
                     /locus_tag="VGP27_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7851775.1"
                     /translation="MTEVLALHDVTFRRDGKQIIDGISLTVNAGEHWALLGPNGAGKS
                     TLLGFCAAVTFPTSGSVHILGEQMGRIELAKLRRFIGHVNPRHRLQYSLTVREVVRTG
                     ITATIDTPMRWTPSAEERGRADSMIAAVGLTHKADDVWPTLSQGERGRTLIARALISE
                     PRLLLLDEPTTGLDVAAREQLLETIDSLDETHPEVASILVTHHLEELPTTTTHALLIA
                     EGRTVATGGARTTVTTDNVSAAFAHPVVVGYDEGRWTARAKATRIIEP"
     gene            complement(1278..2279)
                     /locus_tag="VGP27_10520"
     CDS             complement(1278..2279)
                     /locus_tag="VGP27_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851776.1"
                     /translation="MRKVAALAAAAVTVVALRRAATLRTSMRAAAPELRTLAVLVTGI
                     PMNSMTLPFVRLLMSMKSRPGSGVTVTEHHVEKVEVLVLKLTDGPSPRPAVLLLHGGG
                     MCAGSAQLEAEPAARIVRAIGAVVVSPNYRLAPEHPFPGGLDDCMATLHWMVEHCDEL
                     GIDAGRIAVCGTSAGGGLAAAVAQRAFDEGISLRAQALVSPMLDDRTALRSDLAGKGE
                     LTWSPRSNRWAWTMYLGRRPRMSDAPEYAAPARRADVAGLPAAWIGVGDLETFYDECV
                     AYAHKLTAAGVCCGLVTVPGMYHTAEGIARNTLSMQEFHANMVQFLRVHLGGAPISG"
     gene            complement(2284..>2537)
                     /gene="hisI"
                     /locus_tag="VGP27_10525"
     CDS             complement(2284..>2537)
                     /gene="hisI"
                     /locus_tag="VGP27_10525"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV7851777.1"
                     /translation="MVAWMDDAALARTLETREATYFSRSRGEQWIKGQTSGHTQHVHS
                     VRLDCDGDTVLLEVDQVGAACHTGDHTCFDADLLLAPTD"
BASE COUNT          442 a          875 c          812 g          408 t
ORIGIN      
        1 gggtgctgtg cgaagcctca catcgctact cgccgacggc ccgatcgtgc tgttcttcta
       61 ccccgcggcc atgacgccgg gctgcacgaa ggaggcctgt catttccgcg acctcgccgg
      121 cgagttcaca gccgtcggcg cctcccgggt cggcatcagc accgatcctg tcgccaaaca
      181 ggcgaagttc tccgataccc agcgcttcga ctacccgctg ctgtcggacg ccgacggcag
      241 cgtcgcgggg cagttcggcg tcaagcgtgg gttgttgggc aagctgatgc cggtcaagcg
      301 caccaccttc gtcatagaca ccgacggcac cgtcctcgac gtgatcgcga gtgagttcag
      361 catggaaacc cacgccgata aggcgttgga ggttctgcgg cagcggcagt cggcgtgaca
      421 gaagttctcg cactccacga tgtgacgttc cgccgcgacg gcaagcaaat catcgacggc
      481 atctcgctga cggtgaatgc cggtgagcac tgggccctgc tgggccccaa cggagcaggc
      541 aagagcacgc tgctcggctt ctgtgcggcg gtgacgttcc cgacgtcggg gtcggtgcac
      601 attctcggcg agcagatggg ccggatcgag ctggccaagc tgcgccgttt catcggacac
      661 gtgaatcccc gtcaccgcct tcagtattcg ctgaccgtcc gcgaggtggt gaggaccggc
      721 atcacggcca ccatcgacac cccgatgcgc tggacgccga gcgccgagga gcgcggccgg
      781 gccgactcga tgatcgcggc ggtcgggctg acccacaagg ccgacgatgt gtggccgacg
      841 ctgtcgcagg gtgagcgagg ccgcacactc atcgcccgcg cgctcatctc cgagccgcga
      901 ctgctgctgc tcgacgagcc gaccaccggc ctcgatgtgg ccgcacgcga acagcttctg
      961 gaaaccatcg actcgctcga cgaaactcac ccggaagtcg cgtcgatcct cgtcacgcac
     1021 catctcgaag agcttccgac gacgacgacg cacgcgctgc tgatcgccga gggtcgaacg
     1081 gtggcgaccg gtggggcgag aacgacggtg acgaccgaca atgtcagcgc cgcgttcgcc
     1141 catcccgtgg tggtgggcta cgacgagggt cgctggacgg cgcgggccaa ggccacccgc
     1201 atcatcgagc cgtagcgctc gcgattttgc gccgacatct gcaccagggt tgtggcgcgg
     1261 gccccggata gcagccctca cccagaaatc ggcgccccgc ccaggtggac acgcagaaac
     1321 tgcaccatgt tggcgtgaaa ctcttgcatt gacaacgtgt ttcgagctat tccttcggct
     1381 gtgtggtaca tcccgggcac cgtgaccagc ccgcaacaca cgccggccgc cgtcagcttg
     1441 tgggcgtagg cgacgcactc gtcgtagaac gtctccaggt cgccgactcc gatccacgcc
     1501 gccggcaacc cggcaacgtc ggcgcgccgc gcgggcgcgg catactccgg agcgtccgac
     1561 atccttggcc tgcgtccgag atacatggtc catgcccatc tgttggaccg cggtgaccac
     1621 gtcagctcgc ccttgccggc gaggtcgctt cgcagggcgg tccgatcgtc gagcatcgga
     1681 gacaccagcg cctgggcccg cagggagatg ccctcgtcga aggcgcgctg ggcgaccgcc
     1741 gcagcgaggc cgccgcctgc acttgttccg cacacggcga tacgacccgc atcgatgccg
     1801 agttcgtcgc agtgttcgac catccagtgc agcgttgcca tgcagtcgtc cagcccaccc
     1861 gggaagggat gctccggcgc cagccggtag ttgggtgaca ccacgaccgc accgatcgcc
     1921 cggacgatcc ttgcggcggg ctcggcctcc aactgtgctg agcccgcaca cattccgccg
     1981 ccatgaagca acagcaccgc aggtcgaggc gaaggaccat ctgtcagctt cagtacgagc
     2041 acctcgacct tttcgacgtg gtgttccgtc accgtcacac cggacccggg acgggacttc
     2101 atgctcatca acaatcggac aaagggcagc gtcatcgaat tcatcggaat tcccgtgacg
     2161 agcaccgcca acgtacgcaa ctccggagcc gctgccctca ttgatgtgcg cagagtcgca
     2221 gcgcgacgga gtgcgaccac ggtcaccgcc gcagcggcca gggcggcgac cttcctcaat
     2281 gccctagtcc gtcggggcga gcagcaggtc ggcatcgaag caggtgtggt cgccggtgtg
     2341 gcaggcggcg ccgacctggt cgacttcgag cagcacggtg tcaccgtcgc agtccagccg
     2401 gacggagtgc acgtgctgcg tatggcccga tgtctgcccc ttgatccact gctcgccgcg
     2461 ggaccgcgaa aagtacgtcg cttcgcgcgt ttccagtgtg cgggcaaggg cggcgtcatc
     2521 catccacgcc accatca
//
LOCUS       DASYLX010000428         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2981413,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000428 DASYLX010000000
VERSION     DASYLX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981413"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGP27_10530"
     CDS             complement(<1..270)
                     /locus_tag="VGP27_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEV7851778.1"
                     /translation="MTTHRYSVKVTWRGNTGSGTTGYRDYRRDTVTAAGAKAELPGSS
                     DPAFRGDPNRWNPEELLLASLSQCHLLWYLDLAVRAGVVVTDYADD"
     gene            446..1579
                     /locus_tag="VGP27_10535"
     CDS             446..1579
                     /locus_tag="VGP27_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7851779.1"
                     /translation="MSRKRVVIAGLGDSGVLTAIRLARRFDIVGISAKPALVSGQELG
                     WRLARPEEWARANWIPFDRFRALDRVRTVHGTLTNVDLESRTVAVRADDGTTSQQPYD
                     ALVISTGVTNGFWRTPNLQSHNQIGEDLREAHKRLIDATSVIVIGGGAAAISSAAQVA
                     TTWPDKRVDLYFPGERALPSHHPRIWATVQRRLGDAGVGLHPGHRAVIPDGFTCDAIT
                     REPVAWSTGQPQASADAVLWAIGRVRPNTDWLPAELLDEQGFVEVTPQLQVPGHSNVF
                     AVGDVAATDPLRSSARNRGDGVLARNIRAQFAGKPLRSYRAPKRRWGSVLGVQPDGLE
                     VFTPKGQAFRFPAWSFDRVLMPVIVRWGIYRGVRDNRPISLAP"
     gene            1576..>1702
                     /locus_tag="VGP27_10540"
     CDS             1576..>1702
                     /locus_tag="VGP27_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEV7851780.1"
                     /translation="MTAKWTPARLGDLSGKRIIVTGATNGVGLGTARALANAGAHV"
BASE COUNT          266 a          588 c          587 g          261 t
ORIGIN      
        1 atcgtcggcg taatcggtca ccacgacgcc ggcccgcacg gccaggtcga gataccagag
       61 cagatggcac tgcgacagcg aggcgagcag aagttcctcg gggttccacc ggttcggatc
      121 gccacggaac gccgggtcgg acgatccggg cagttcggcc ttggcgcccg ccgcagtaac
      181 ggtgtcccgc cggtaatccc ggtagccggt ggtgcccgac ccggtattgc cgcgccacgt
      241 cacttttacc gagtagcggt gtgtcgtcat ggtgcgtcgc cttccgtgaa tcggcgcgca
      301 ccgtgttcca gcggcaggcg cagcgcattg accaggtagc cgatcatctt cgcgcgcgcc
      361 aggctgccgg cgagatgaac ggagaagcac tcgtctcggc ccgtaatgcc ttgcaggacc
      421 cgagcaccgt gaatgataag cggccatgag ccgcaaacgt gtcgtgatcg cggggctggg
      481 cgacagcggg gttctcaccg ccatccgact ggcccggcgg ttcgacatcg tcggcatctc
      541 cgccaagccg gcgttggtga gcggccagga actggggtgg cggctcgccc ggccggagga
      601 atgggccagg gccaactgga ttccgttcga ccggttccgt gcactggaca gggtgcgcac
      661 cgtgcacggc accctgacca atgtcgacct cgaatcccgc acggtggcgg tccgcgctga
      721 cgacggcacg acatcgcagc agccctacga cgcgctggtc atctccaccg gggtcaccaa
      781 tggtttctgg cgcacgccga acctgcagtc gcacaaccag atcggcgagg acctccggga
      841 agcgcacaag cgcctgatcg acgccacgtc ggtcatcgtc atcggtggtg gcgcggcggc
      901 gatcagtagc gccgcacagg tcgccaccac ctggccggac aagcgcgtcg acctctattt
      961 ccccggtgag cgcgccctcc cgtcacacca tccgcggatc tgggcgacgg tgcagcgccg
     1021 gctcggcgac gccggcgtgg gactgcaccc cggccaccga gcggtgatcc ccgacggctt
     1081 cacgtgcgat gcgatcaccc gcgagccggt tgcgtggagc accgggcagc cgcaggcgtc
     1141 cgccgatgcc gtcctgtggg cgatcggtcg ggtcaggcct aacaccgact ggctgccggc
     1201 cgaactcctc gacgaacagg gcttcgtcga agtgacaccg cagttgcagg tgcccggcca
     1261 ctcgaacgtg ttcgcggtgg gcgacgtggc ggcgaccgat ccgctgcgca gctcggcgcg
     1321 caaccgcggg gacggggtgc tggcccgcaa catccgcgcg cagttcgcag gcaaaccgct
     1381 gcgctcctac cgggcgccga agcggcgttg gggttcggtg ctcggtgtgc agcccgacgg
     1441 gctcgaggtg ttcaccccca agggtcaggc gttccgcttc ccggcctggt ctttcgaccg
     1501 cgtcttgatg ccagtgatcg tccgatgggg tatctatcgc ggtgtgcgcg acaaccggcc
     1561 gattagcctt gccccgtgac ggcgaagtgg acaccggccc ggctgggcga cctctcgggt
     1621 aagcgcatca tcgtgaccgg cgcgacgaac ggcgtcggcc tcggcaccgc ccgcgcgctc
     1681 gccaacgcgg gcgcacacgt cg
//
LOCUS       DASYLX010000429         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_568730,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000429 DASYLX010000000
VERSION     DASYLX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568730"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGP27_10545"
     CDS             <1..606
                     /locus_tag="VGP27_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851781.1"
                     /translation="PMLVVDYRVAPEHPHPTPVEDCYAALEWLAGQATTLGFDPARLA
                     VMGDSAGGGLAAGVCLMARDRGGPTVAQQLLIYPMLDDRPSAPDSALLRFLTWTYDDN
                     LTGWGALLGDAAGGDDVSPYAAPARAADLAGLPDTYIDVGDLDLFRDEDITYARRLSD
                     AGVPTELHLHPGCPHAFEALADGTDVAERAINDRIRRLESL"
     gene            770..1186
                     /locus_tag="VGP27_10550"
     CDS             770..1186
                     /locus_tag="VGP27_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851782.1"
                     /translation="MKLARTLVNAAVVAALGLGAGLGAGVASAKPGHGNDIDRPCFPY
                     CENGRGHNDWNDFNHADFDRGPWWANNRHDWWDDRQGPPPWGWGPPPAYQWAGGPPRP
                     FNYWGYDVNPVWDDGFHQWGIWLLGLWIPIFGIGYG"
     gene            complement(1183..1644)
                     /locus_tag="VGP27_10555"
     CDS             complement(1183..1644)
                     /locus_tag="VGP27_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851783.1"
                     /translation="MQASVTVPMAASADEVCNIVADVRNTGKFSPEVIEAEWLDGATG
                     PALGARFRGHVRRNEIGPVYWTTCRVTACEPGREFGFEVLVGDRPVNNWHYRFTPTDD
                     GTDVTESFRMNDSVFTKLFAMLGGQLRTRRNKRDMRKTLERIKAVVETPSA"
     gene            1685..2455
                     /locus_tag="VGP27_10560"
     CDS             1685..2455
                     /locus_tag="VGP27_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7851784.1"
                     /translation="MTDWSGEDYSKISSLQRAMIEDAMASLVFADGDRVLDVGCGDGH
                     LTHEMARMVSSGCAIGVDPSKRMIATAHAAGAPTMSGPWFVIADARRLPFGEHFDVVV
                     SFNALHWVPEQQQALRQIASVLRPGGCAVIQVVCAGQRQSLEEVAMQTCRRPGWASLF
                     DGFAAPFVHVEADAYGELAASAGLSLTNLTVTDHDWDYGSRSAFRQWCAVGTTSWTDR
                     LPVEDRGLFVDALVDGYEPVAGGTALFRFTQMRAELRR"
     gene            2639..3100
                     /locus_tag="VGP27_10565"
     CDS             2639..3100
                     /locus_tag="VGP27_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV7851785.1"
                     /translation="MAAAVVAVSLSSPPAALADDRLDFTGTTLSGAPFNGSSLQGKPA
                     VLWFWTPWCPFCNAEAPSVSSAAAANPKVTFVGVAARSDVAAMQGFVSKYNLGFTNLD
                     DADGSVWARYNVPWQPAYVFYRADGTSTFVNNPTSAMPAQELQERVSALTA"
BASE COUNT          474 a         1010 c         1070 g          563 t
ORIGIN      
        1 ccgatgcttg tcgtcgatta ccgggtggcg cccgagcacc cgcatcccac accggtcgag
       61 gactgttatg cggcgctcga atggctggcc gggcaggcga cgacgctcgg tttcgatccg
      121 gcccggttgg ccgtgatggg cgacagcgca ggtggtgggc tggccgccgg tgtgtgcctg
      181 atggcccgcg accgtggtgg ccccaccgtg gcgcaacagc tgctgatcta cccgatgctg
      241 gatgaccggc cttcggcgcc tgactccgcc ttgctgcggt tcctcacctg gacttacgac
      301 gacaacctca ccggctgggg ggcgctgctc ggcgacgcag ccggtggtga cgacgtgtcg
      361 ccgtatgccg cgcccgcccg cgcggccgac ctggccgggc ttcccgacac ctacatcgac
      421 gtgggcgacc tggacctctt tcgcgacgag gacatcacct acgcacgcag gctgtccgat
      481 gccggagtgc ccaccgagct tcatctgcat cccggttgcc cgcacgcctt cgaagcgctg
      541 gccgacggta ccgacgttgc ggagcgggcg atcaacgacc gcatccgccg ccttgagtcc
      601 ctgtgaccac gggaaggtaa cggtcggtga gcgccgctcg ataacccggt gggggtcgtg
      661 gcttgcgctc atgccacttg atgcaggcca ggggaatgac cggtggcgca tgcgcgtcga
      721 agaggtggga acgattcggc attcgcgtca ccccaacgag gagttgagaa tgaaactcgc
      781 caggacattg gtcaacgctg cggtcgtcgc tgcgctcggc ctcggggccg ggctgggcgc
      841 gggcgtggcg tcggccaagc cgggacacgg caacgacatc gatcggccgt gcttcccata
      901 ctgtgagaat gggcgcggcc acaacgattg gaacgacttc aaccacgctg acttcgacag
      961 gggtccgtgg tgggccaaca atcgccacga ctggtgggac gaccgccagg gcccgccgcc
     1021 gtggggatgg ggtccgccgc cggcatacca gtgggccgga ggcccgccgc gacccttcaa
     1081 ctactggggc tacgacgtca acccggtgtg ggatgacggc ttccatcagt gggggatctg
     1141 gctgttaggc ctgtggattc cgatcttcgg catcggctac ggctaagccg acggggtttc
     1201 caccacggcc ttgatccgct ccagcgtctt gcgcatgtcg cgtttgttgc gccgcgtgcg
     1261 gagttgaccg ccgagcatcg cgaagagttt ggtgaacacg ctgtcgttca tccggaacga
     1321 ttcggtgacg tcggtgccgt cgtccgtcgg cgtgaaacgg tagtgccagt tgttgaccgg
     1381 ccggtcgccg acgagcacct cgaagccgaa ctcccggccc ggctcgcagg cagtgacccg
     1441 gcacgtcgtc cagtagaccg gaccgatctc gttgcggcgc acgtgtccac ggaaccgtgc
     1501 gccgagcgcg ggtccggtgg cgccgtccag ccattcggcc tcgatcacct ccggcgagaa
     1561 cttcccggtg ttgcggacgt cggcgacgat gttgcagacc tcgtccgccg atgccgccat
     1621 cggaactgtc acagatgcct gcattgagac ctccttagtt gccggtatca cgttaactgt
     1681 gcgggtgacc gactggagcg gcgaggacta ctccaagatc agctcgctgc agcgggcgat
     1741 gatcgaggac gctatggcga gcctcgtgtt cgccgacggg gaccgcgtgc tcgacgtcgg
     1801 ttgcggtgac ggccatctca ctcatgagat ggcgcgcatg gtgtcgtccg ggtgcgcgat
     1861 cggcgttgac ccgtcgaagc gaatgatcgc cacggcgcac gccgcgggcg cgccgacgat
     1921 gtcgggaccc tggttcgtca ttgccgacgc ccgcaggctg cccttcggtg agcacttcga
     1981 cgtcgtcgtg tcgttcaacg ctctgcattg ggtgcccgaa caacagcagg cgctgcggca
     2041 gatcgcctcg gtgttgcggc cagggggctg cgccgtgata caggtggtct gcgcggggca
     2101 gcgccaaagc ctggaggagg tggcgatgca gacttgccga cgtcccggct gggcttcctt
     2161 gttcgacggg ttcgccgcac ccttcgtcca cgtcgaagcg gacgcgtacg gcgagctcgc
     2221 ggcgtcggcg ggactcagct tgaccaatct gactgtcacc gaccacgact gggattacgg
     2281 gtcgcgcagc gcgtttcggc aatggtgtgc ggtcggcacg acctcatgga ccgatcgctt
     2341 accagtcgag gaccggggct tgttcgtcga cgcgctcgtc gatgggtacg agcccgttgc
     2401 gggcgggacc gctctgttcc gattcaccca gatgcgggcc gaactccgac gctaggcggc
     2461 catctcgtcg tacagcggcg ccatccgttc caggaaccag gcctttcgcc ttcgcgggga
     2521 attgaaccgg aaaccggggg aactcgttct acctaatgtc accccgtcgg tgtgggcgca
     2581 gggaggagcc gtcatgtttg gtcgtctact acattcgagc aggtcgctgg ctgtggctat
     2641 ggccgctgcc gtcgtcgcgg tgtcgttgag cagcccgccg gcggctctgg ccgacgatcg
     2701 cctcgacttc accggaacga cgctgagcgg cgcgccgttc aacgggtcgt cactgcaggg
     2761 caagcctgcg gtgctctggt tctggacacc ctggtgcccg ttctgcaatg ccgaggcacc
     2821 gtcggtgagc agcgcggcgg ccgccaatcc caaggtcacc ttcgtcgggg tggccgcgcg
     2881 ctccgacgtg gcggcgatgc agggcttcgt gtcgaagtac aacctcggtt tcaccaatct
     2941 cgacgacgcc gacggttccg tctgggcgcg ttacaacgtg ccgtggcagc cggcgtatgt
     3001 gttctatcgc gccgacggca catcgacgtt tgtcaacaac ccgacttcgg cgatgcctgc
     3061 gcaggaactg caggagcggg tctcggcact caccgcgtaa ccggcgcgcc gcgtgca
//
LOCUS       DASYLX010000430         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2542399,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000430 DASYLX010000000
VERSION     DASYLX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542399"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGP27_10570"
     CDS             complement(<1..467)
                     /locus_tag="VGP27_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV7851786.1"
                     /translation="MTSDARPRTMAEKVWGDHVVALGGGTGDAREPDLIYIDLHLIHE
                     VTSPQAFDGLRLAGRGVRRPDLTIATEDHNVPTEDIDKPIADPVSRTQVETLRRNCEE
                     FGIRLHPMGDSEQGIVHIIGPQLGLTQPGMTVVCGDSHTSTHGAFGSLAMGIGT"
     gene            507..1208
                     /locus_tag="VGP27_10575"
     CDS             507..1208
                     /locus_tag="VGP27_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7851787.1"
                     /translation="MRQDSGIGVLDKAVGLLHTVAESPCALAELCERTGLPRATAHRL
                     AAGLEVHRLLARDAEGRWRLGPALVELAGQVNDPLLAAGAAVLPRLREITGESVQLYR
                     REGTSRICVAALEPPAGLRDTVPVGIHLPMTAGSGAKILLAYSDAATQQAVLPTAKFT
                     DRALAEVRKRGWAQSVAEREPGVASVSAPVRDGRGQVIAAVSVSGPIDRIGRRPGARW
                     AADLLAAADALARRL"
     gene            complement(1215..1290)
                     /locus_tag="VGP27_10580"
     tRNA            complement(1215..1290)
                     /locus_tag="VGP27_10580"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1254..1256),aa:Glu,seq:ctc)
     gene            complement(1353..1424)
                     /locus_tag="VGP27_10585"
     tRNA            complement(1353..1424)
                     /locus_tag="VGP27_10585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Gln,seq:ctg)
     gene            complement(1542..>2102)
                     /locus_tag="VGP27_10590"
     CDS             complement(1542..>2102)
                     /locus_tag="VGP27_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV7851788.1"
                     /translation="DVNSSPARFDQKKADALNADHIRLLTEDEFTRRLRAYFDTHAYD
                     TGLGDQQFAEAARLVQTRIVVLGDAWDLLKFLNDDEFTLDEKAAAKELGPDAAAVLDA
                     ALHALEGVDGWRVDTIEAALKAALLEDLALKPRKAFGPIRVAVTGASVSPPLFESLEL
                     LGRDRSLQRLRAARDRAAAAAAATSG"
BASE COUNT          331 a          701 c          718 g          352 t
ORIGIN      
        1 gtgccgatgc ccatcgcgag cgaaccgaat gcaccgtggg tcgacgtgtg actgtcgccg
       61 cagacgaccg tcatacccgg ctgagtgagg ccgagttgcg gtccgatgat gtgcacgatg
      121 ccttgctcgg aatcgcccat cggatgcagg cggatgccga actcctcaca attgcgtcgc
      181 agcgtttcga cttgagtgcg cgagaccgga tcggcgatcg gcttgtcgat gtcttccgtc
      241 ggcacgttgt ggtcttcggt cgcgatggtc agatccggtc gccggacacc gcggcccgcg
      301 agacgcaacc cgtcgaacgc ctgcgggctg gtgacctcat ggatgagatg gaggtcgatg
      361 tagatcagat cgggttcgcg ggcatctccc gtgccgccgc cgagggcgac gacgtggtcg
      421 ccccatacct tctccgccat ggtgcgcggt cgcgcgtcgc tcgtcatact tgaccatctc
      481 acattctgga acgctagtat ctccttgtga gacaggatag cggtatcggc gtgctggaca
      541 aagcggttgg gctgctgcac acggtggccg agtcgccctg cgcgctggcc gaactgtgcg
      601 agcggacggg cctgccccgg gccaccgcgc accggctcgc cgccgggctg gaggtccacc
      661 ggctgctcgc ccgcgacgcc gagggccgct ggcggctcgg ccccgcgctg gtcgagctgg
      721 caggccaagt caacgacccg ctgctggccg cgggggccgc cgtgttgccg aggctgcgcg
      781 aaatcaccgg cgaaagcgtt cagctctatc gccgcgaggg cacatcgcgg atctgcgttg
      841 ccgcactgga accgcctgcg ggacttcgcg ataccgtccc ggtgggcata cacctgccga
      901 tgaccgccgg ctctggcgcg aagatcctgc tggcgtacag cgatgccgcg acgcagcagg
      961 cggtgctgcc caccgccaag ttcaccgacc gcgcactggc ggaggtacgc aagcgcggct
     1021 gggcgcaaag cgttgccgaa cgcgagccgg gcgtggcgag cgtctcagcg ccagtgcgcg
     1081 acgggcgggg tcaagtgatc gcggctgtat cggtgtcggg gccgatcgat cggatagggc
     1141 gccggccggg cgctcgatgg gcggcggacc tgctggccgc cgcggacgca ctggcccgcc
     1201 gactgtgaaa aaagtggtac ccccgatggg attcgaaccc acgctaccgc cgtgagaggg
     1261 cggcgtccta ggccgctaga cgacgggggc cagaactttc cgaagggcca gcatagctca
     1321 gtcgccaatg ccggcccaat tcgttcgcac tgctggggta ccaggactcg aacctagaat
     1381 ggcggtacca gaaaccgctg tgttgccaat tacaccatac cccattggct gcctgtaacc
     1441 gctggtcaca agctatttcc gggccaagca acccctgagc gagcaactcg cctgtgatgc
     1501 cgggccgacg tgcagattac caaagatttc gagtccgctt ctcatccgct ggtcgctgcc
     1561 gctgccgccg ccgcacggtc gcgggcggcg cgcaggcgct gcaggctgcg gtcacggccg
     1621 agcagctcca gcgactcgaa cagcggcggg ctgaccgacg ctccggtcac ggccacccgg
     1681 atcggaccga acgccttgcg cggcttgagt gccaggtcct ccaacagcgc agccttgagg
     1741 gccgcttcga tcgtgtcgac gcgccatccg tcgacgccct cgagcgcatg cagcgcggcg
     1801 tcgaggaccg cagctgcgtc ggggcccaat tccttcgccg cggccttctc gtcgagcgtg
     1861 aactcgtcgt cgttgaggaa cttcagcagg tcccacgcat ccccgagcac gacgatgcgg
     1921 gtctgcacca ggcgtgcggc ctcggcgaac tgttgatccc ccagccctgt gtcgtatgcg
     1981 tgggtgtcga agtacgcccg caaccgtcgg gtgaactcgt cctcggtcag cagccggatg
     2041 tgatcggcgt tcagcgcgtc ggccttcttc tggtcgaagc gggccgggct ggagttgacg
     2101 tc
//
LOCUS       DASYLX010000431         6801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3639905,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000431 DASYLX010000000
VERSION     DASYLX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3639905"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGP27_10595"
     CDS             complement(<1..353)
                     /locus_tag="VGP27_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV7851789.1"
                     /translation="MPSGLIAALGAPIHEVAAQVGPGWSDEGDPTTSLAEVRDLLADA
                     AAAARNGWSRAADGWTGAGADGAADFVSATADAADRLALRIDEMRAGAAGAADAVAWA
                     RSRLRDIINRFEERAT"
     gene            complement(356..643)
                     /locus_tag="VGP27_10600"
     CDS             complement(356..643)
                     /locus_tag="VGP27_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851790.1"
                     /translation="MLVDTNAIRALGADCSTQADHLSAAASALTSLPGPDAAAAFGRV
                     GAHFLATLADAAVAEARAITALSDNLASAHSISGVLADAYAETDRRGSRLL"
     gene            698..1321
                     /gene="upp"
                     /locus_tag="VGP27_10605"
     CDS             698..1321
                     /gene="upp"
                     /locus_tag="VGP27_10605"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV7851791.1"
                     /translation="MDVRVVDHPLAAARLTTLRDERTNNAAFRDALRDLTLMLVYEAT
                     RDAATESVPVRTPVADTTGARLANPPLLVPVLRAGLGMVDQAHALIPEAHVGFVGVAR
                     DEETHQPRPYLESLPDDLRTTPVFVLDPMLATGGSMAHTIGLLQARNAVDITAICVVV
                     APEGIAAIDKVAPDLRLFTATIDDCLNDIAYIVPGLGDAGDRQFGPR"
     gene            complement(1318..2961)
                     /locus_tag="VGP27_10610"
     CDS             complement(1318..2961)
                     /locus_tag="VGP27_10610"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV7851792.1"
                     /translation="MTESSLGSTVREWIEHDPDPETVAELKACSDDELEERFARPLTF
                     GTAGLRGPLRGGPDGMNLAVVLRTTWAVAQVLKERDHAGSRVVVGGDARHGSETFALA
                     AAEVLAAQGFMVTLMFAAVPTPVVAFGARHLGASAGIQITASHNPSSDNGYKVYFEGG
                     FQIVSPTDREIEAMICKAPHADQIPRAAVQTSGMDLIQRYVDRTSHVRRTHGSARLVF
                     TAMHGVGGEYALDAFVRAGFSDVQVVESQFAPDPDFPTVPFPNPEEPGATDELLKLAA
                     DTEAEVAIALDPDADRCAVGVPTPDGWRMLSGDETGWLLGDYILSQIDPGEVSEHTVV
                     ASSVVSSRMLSRIAAAHGARHAETLTGFKWLSRADEDLPGCTLVFAYEEAIGYCVDPS
                     AVRDKDGISTAVLFCDLVVALREQGRTVLDALDDLARMHGVHATTAVSRPVAGADEAA
                     EVMTRLRSRPPTKLCGFDVTMTDLAAVRPYERRTDAVILEGGDGGTAVRVAVRPSGTE
                     PKVKSYIEVRRKCVDGDVTAARAQARLLAEELAELAGRF"
     gene            complement(3023..3508)
                     /locus_tag="VGP27_10615"
     CDS             complement(3023..3508)
                     /locus_tag="VGP27_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851793.1"
                     /translation="MTEVRAGSPATELRESMMAVTRQLRRHRADHGLTLTQMEVLGEV
                     SRAPRTRAASGGGVSTPAELAARLHVRVQSLTDSINELVSRGLVLRRPDDDDRRRQLI
                     EITKAGIELLDDDRAERDAWLSATMRENLTDLESNLLMLVAPVLRKLAYAEGKVGTLA
                     V"
     gene            3598..4722
                     /locus_tag="VGP27_10620"
     CDS             3598..4722
                     /locus_tag="VGP27_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV7851794.1"
                     /translation="MKERLKPVARWALLVAITIAVTVPLTMVGVPSAALFAALVVGIA
                     LALPGVAPQRVFRPVGIAAQGVLGVYIGTMVDRDAVSALGPHWVIVLAVAVATLVLSI
                     VAGALLGLHRTVTPLTGSLALVAGGASGLVAIARELGGDDRVVAVVQYLRVALVTASM
                     PVVVTLIYHADRSHHAAAITQTDSAPWYLSVAVMAVLIVVGAAGGRLVKLPGSGLLGP
                     MALTVALQLSGLSFGLSVPSVLVQVGYMLIGWQAGVAFTRDSLRAVGRLLPAAIGLIV
                     VIGVGTAGLGVVLADIAGLTPLEGYLATSPGGVYAVLATAVETDSNVTFVIAAQVVRI
                     LLMLFMAPFLGRAVMWLSNRSRGQSREITEASREPVRVAD"
     gene            complement(4668..5462)
                     /locus_tag="VGP27_10625"
     CDS             complement(4668..5462)
                     /locus_tag="VGP27_10625"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV7851795.1"
                     /translation="MTDPEAVAQQAAAAIRERTGIDTHDVAVILGSGWAPAAAELGEP
                     TAVLPVAELPGFTPPTAEGHGGDVISVRTGDHRVLVFVGRIHAYEGHGLRHVVHPVRA
                     ACAAGVRTLVLTNAAGGLRADFAVGQPVLISDHLNLTARSPLVGAQFVDLVDAYSPRL
                     RALAREVDPDLAEGVYACLPGPHYETPAEITMLRTLGADLVGMSTVHETIAARAAGAE
                     VLAVSLVTNAAAGMTGEPLNHEEVLAAGRQSATRTGSLLASVISRL"
     gene            5500..6684
                     /locus_tag="VGP27_10630"
     CDS             5500..6684
                     /locus_tag="VGP27_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV7851796.1"
                     /translation="MPTTTAAVSVEDAINRRRGDLIELSHSIHSEPELAFAEYKSCAK
                     TQALVAERGFDVTAATGGLDTAFRAEFGSGALIIGICAEYDALPGLGHACGHNIIAAS
                     AVGTALALADVADELGLTVVLLGTPAEEAGGGKVLLLNAGTFDDIAATVMLHPGPIDI
                     ARARSLALSQVEVTYRGREAHAAVAPYLGVNAADAITVAQTAIGLLRQQFVPGQMAHG
                     IVTDGGQATNVIPARTVLRYTMRATDAPSLHHLEGKMADCFLAGAIATGCDYEVEETE
                     PPYDELAPDKWLAGVFRDEMIRLGRAPVGEEIEAALPLGSTDMGNVTQLMPGIHPVVG
                     VDAEGASVHQPGFATAAASASGDEAVVEGAIMLARTVVALAETPGERDRVLESQARRV
                     AS"
     gene            6681..>6801
                     /locus_tag="VGP27_10635"
     CDS             6681..>6801
                     /locus_tag="VGP27_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7851797.1"
                     /translation="MTLAGAADSWLADHYDDVVAWRRHIHMHPELGRQEFATTQ"
BASE COUNT         1015 a         2362 c         2291 g         1133 t
ORIGIN      
        1 gtcgcgcgtt cctcgaaccg gttgatgatg tcccgcagcc gcgagcgggc ccatgccacg
       61 gcatccgcgg caccggcagc gcctgcccgc atttcgtcga tccgcagcgc caatctgtcg
      121 gctgcgtctg ccgtggcgga cacgaagtcg gccgccccgt cggcgcctgc gcccgtccac
      181 ccgtcggcgg ctcggctcca accgttgcgt gccgcggcgg cggcgtcggc cagcagatcg
      241 cgcacctcgg cgagcgaggt ggtcggatcc ccctcgtccg accaccccgg tccgacctgc
      301 gccgctacct cgtgaatggg cgcccccagc gccgcgatca gcccgctcgg catcgctaga
      361 gcaggcggct gccgcgccgg tcggtttcgg cgtaggcgtc tgccagcacc cccgagatcg
      421 agtgggccga cgcgagattg tcgctgagcg ccgtgatcgc ccgagcttcc gccacggcgg
      481 cgtcggcgag cgtcgccaag aagtgcgcgc cgacacgacc gaaagcggcg gcggcgtcgg
      541 gacccggcag cgacgtcagc gcgctggcgg cggcggacag gtggtcggcc tgggttgagc
      601 agtccgcgcc gagtgcgcga atggcatttg tatcgacgag catggggata cgacgatcat
      661 ccccgccgac tggttccctt gacgttaggg tcggcccatg gacgtgcgcg tcgtcgacca
      721 cccgctggcc gcggcgcggc tgaccactct gcgcgacgag cgcacgaaca acgcggcgtt
      781 tcgggatgcg ctgcgcgacc tgacgctgat gctcgtgtac gaggccaccc gcgacgcggc
      841 caccgagagc gttccggtgc gcaccccggt tgccgacacc accggagcgc ggctggcgaa
      901 tccgccgctg ctggtgcccg tgctgcgcgc cggcctgggc atggtcgacc aggcgcatgc
      961 gctgattccg gaggcgcacg tcggcttcgt cggagtcgct cgcgacgagg aaacccacca
     1021 gccgaggccg tatctcgaat cactgcccga cgatctgagg accacgccgg tgttcgtact
     1081 cgacccgatg ctggcgaccg gaggctcgat ggcgcacacc atcgggctgc tgcaggctcg
     1141 caacgccgtt gacatcaccg cgatctgcgt cgtcgtcgct ccggaaggca ttgcggcgat
     1201 cgacaaggtc gcaccggacc tcagattgtt caccgcgacg atcgacgact gtctcaacga
     1261 catcgcctat atcgtcccgg gactggggga cgcgggcgac cggcagttcg gtccgcgcta
     1321 gaacctgcct gccagctcgg ccaattcctc ggcgagaaga cgtgcctggg cacgggcggc
     1381 ggtcacgtct ccgtcaacgc atttccgccg aacctcgatg taggacttca ctttcggctc
     1441 ggtacccgac ggccggaccg ccacccgcac tgcggtgccg ccgtctccgc cctcgaggat
     1501 gacggcgtcg gttcgccgct catagggccg cacagccgcc agatctgtca tcgtgacatc
     1561 gaacccgcac agcttcgtcg gtggcctgga ccgcaatcgg gtcatcacct ccgcggcctc
     1621 gtctgcgccg gccaccggcc gtgagacggc ggtcgtggcg tgcacgccgt gcatccgggc
     1681 aaggtcgtcg agggcgtcga gcactgtgcg accctgctcc cgcagcgcga ccaccaagtc
     1741 gcagaacaac accgctgtgc tgatgccgtc cttgtcgcga accgcggacg ggtcgacgca
     1801 gtagccgatc gcctcctcgt atgcgaagac gagcgtgcag cccggtagat cctcgtctgc
     1861 gcgcgacaac cacttgaagc cggtgagagt ttcggcgtgg cgggcgccgt gcgcggccgc
     1921 gatacgggac agcatccgcg acgacaccac cgagctcgcc accacggtgt gttcgctcac
     1981 ctcgccgggg tcgatctggg agaggatgta atcgccgagc agccaaccgg tttcgtcgcc
     2041 gctgagcatg cgccacccgt ccggcgtcgg aactccgaca gcacagcggt cggcgtcggg
     2101 atccaaggcg atggcgactt cggcctcggt gtcggcggcc agcttcaaca gttcgtcggt
     2161 ggcgcccggt tcctccggat tgggaaacgg cacggtgggg aagtcggggt cgggcgcaaa
     2221 ctggctttcg accacctgca cgtcggagaa gccggcccgc acgaatgcat cgagggcgta
     2281 ctcaccgccg acaccgtgca tcgcggtgaa cacaagccgt gccgatccgt gggtgcgccg
     2341 gacatgcgat gtgcgatcca catagcgctg aatcaggtcc attcccgacg tctggaccgc
     2401 tgcccgagga atctggtcgg cgtgcggcgc cttgcagatc atcgcctcga tctctcgatc
     2461 ggtgggcgaa acaatctgga agccgccctc gaagtagacc ttgtacccgt tgtccgacga
     2521 cggattgtgc gacgccgtga tctgaatacc cgcgctcgcg ccgagatggc gggcgccgaa
     2581 cgccaccacg ggagtcggga cggcggcgaa catcaacgtc accatgaaac cctgggccgc
     2641 aagaacttcg gctgccgcga gggcgaaggt ctcggagccg tgccgggcgt caccgccgac
     2701 caccacacgt gatccggcgt ggtctcgctc cttgagcacc tgcgcgactg cccacgtggt
     2761 tctcaggacg accgcgaggt tcattccgtc cggcccgccc cgcaacgggc cccgcaatcc
     2821 ggccgtgccg aatgtcaggg gacgggcaaa ccgttcctcg agctcgtcat cgctgcacgc
     2881 tttcagctcg gcgacggtct ccgggtcggg gtcgtgctcg atccactcac gtacggtcga
     2941 accaagcgac gactccgtca tcggcgttgc tttctcgcgc tcagcgctcg tcatcagggt
     3001 cgcctcctcg cgccagcgct cgtcatacgg ccagtgtgcc cactttgccc tccgcgtaag
     3061 ctagcttgcg cagcaccggc gccaccagca tcagcaagtt ggattcgagg tcggtcaggt
     3121 tctcgcgcat ggttgcgctc agccaggcgt ctcgctcggc gcggtcgtcg tcgagcaact
     3181 cgatacccgc cttcgtgatc tcgatcagct ggcggcgtcg gtcgtcgtcg tcggggcggc
     3241 gcagcaccag accgcggctg accagttcgt tgatgctgtc ggtgagggat tgcacgcgta
     3301 cgtgcagccg agcggccaac tcggcgggcg tgctgacccc gccgccgctt gctgctcgtg
     3361 tgcgcggcgc cctgctgacc tcgccgagca cctccatctg agtgagcgtc aacccgtggt
     3421 ctgcgcggtg cctgcgcaac tgcctggtca ccgccatcat cgactctcgc agctcggtgg
     3481 ccggcgatcc ggcacgcact tcggtcataa ccaagttata ccttggtaat tcttcgccgt
     3541 catcgctaaa acgtcgtgtt gcgtgcgtat tttgttaagg gcatacttgt catagtgatg
     3601 aaagagcgac tcaagccggt agccagatgg gcgttgctgg tcgccatcac catcgccgtc
     3661 accgtcccgc tgaccatggt cggcgttccg tcagccgcgt tgttcgcggc gctggtcgtc
     3721 ggcatcgcgc tggccttgcc cggcgtcgca ccgcagcgcg tgttccgtcc ggtcggcata
     3781 gctgcgcagg gtgtgctggg cgtctacatc ggcacgatgg tcgaccgtga cgcggtgagc
     3841 gcgctcgggc cgcattgggt gatcgtgctc gccgtcgccg tcgccacgct ggtactcagc
     3901 atcgttgcgg gtgcgctgct cggattgcac cggaccgtca cccctctcac cggctcgctg
     3961 gcattggtgg ccggtggggc gtccggcctg gtcgccatcg cccgggagtt gggtggtgac
     4021 gaccgagtgg tcgcggtcgt gcagtacctg cgggttgcgc tggtgaccgc ttcgatgccc
     4081 gtcgtcgtca cgctgatcta tcacgccgac cgatcccacc atgccgcggc gatcacccaa
     4141 accgattcgg ccccttggta tctcagtgtt gctgtgatgg cagttctgat tgtggtcggc
     4201 gccgcgggtg ggcggctggt gaagcttccc ggctccgggc tgctcggccc gatggcgctc
     4261 accgttgcgc ttcagctcag cgggctgtcc ttcggtctat ccgtgcccag tgtgttggtg
     4321 caggtgggct acatgttgat cgggtggcag gcaggtgtcg cgttcacccg cgactcgctt
     4381 cgcgcggtcg ggcgactgct gcctgcggcg atcggactca tcgtggtgat cggtgttggc
     4441 acggccggcc tgggcgtggt gctcgccgac atcgccggcc tgaccccgct cgagggatat
     4501 ctcgccacca gccccggcgg ggtgtacgcc gtgctggcca cggcggtgga gaccgactcg
     4561 aacgtcacct tcgtcatcgc ggcacaggtg gtgcgaatcc tgctgatgct cttcatggcg
     4621 cccttcctgg gccgcgccgt gatgtggctc agcaatcggt cccgcggtca gagccgcgag
     4681 atcaccgagg ccagcaggga acccgtccgg gtcgccgact gacggcccgc ggccaggacc
     4741 tcttcatggt tcagcggctc tcccgtcatg cccgccgccg cattggtcac cagagacacc
     4801 gcgagcacct cggcacccgc cgcccgcgcg gcgatggtct cgtgcaccgt cgacatgccg
     4861 accagatcgg ccccgagcgt gcgcaacatg gtgatctcgg cgggcgtttc gtagtgcggt
     4921 cctggcagac aggcgtagac gccctcggcc agatccggat ccacctcgcg ggcgagcgcc
     4981 cgcaaccgcg gcgagtaagc gtcgaccaag tcgacgaact gcgccccgac caacggcgac
     5041 ctcgcggtca gattcaagtg gtcgctgatc agcacgggct ggccgaccgc gaagtccgct
     5101 cgcaggccgc ccgcggcgtt ggtcagcaca agtgttcgca caccggccgc acacgcggcg
     5161 cgcaccgggt gcacgacatg gcgcaggccg tgtccttcgt aggcgtggat cctgccgacg
     5221 aacaccagca cccggtggtc gcccgttcgg accgagatga cgtcgccgcc gtgcccctcc
     5281 gcggtcgggg gagtgaagcc gggcagttcg gcaacgggga gcaccgccgt cggctcaccc
     5341 aactcggcgg ccgcaggcgc ccatccggag ccgaggatca ccgcgacgtc atgggtgtcg
     5401 ataccggtgc gttcgcggat cgcggctgcg gcctgctgcg ctaccgcctc cggatcggtc
     5461 acagttcggc agcttagccg tcagtgagat actgcgagga tgcccaccac caccgccgcc
     5521 gtcagcgtcg aagacgcgat caaccggcgc cgtggtgacc tgatcgagct gtcgcactcg
     5581 atccattccg agcccgagct ggcgttcgct gagtacaaga gttgcgccaa gacgcaggcg
     5641 ttggtcgccg aacgcggttt cgatgtcacc gctgccaccg gcgggttgga caccgccttc
     5701 cgtgccgagt tcggcagcgg tgcattgatc atcggcatct gcgccgaata cgacgcgctg
     5761 cccggtctcg gccacgcctg cggccacaac atcatcgcgg cctcggcggt cgggaccgcc
     5821 ctcgcactgg ctgacgtggc cgacgaactc ggcctgacgg ttgtgctgct gggtacgccc
     5881 gccgaggagg ccggcggcgg aaaagtgttg ctgctcaacg ccggaacgtt cgatgacatc
     5941 gctgcgacgg tgatgctgca tcccggcccg atcgacatcg cgcgggcgcg ctcgctggcg
     6001 ttgtctcagg tcgaagtcac ctaccgtggc cgcgaggcgc acgcggccgt cgcaccgtat
     6061 ctgggcgtca acgcggccga cgcgatcacc gtggcgcaga cggccattgg gctgctgcgt
     6121 cagcagttcg tgccgggtca gatggcgcac ggcatcgtca ccgacggcgg tcaggccacc
     6181 aacgtcatcc ccgcccgcac tgtcctgcgc tacacgatgc gcgcgaccga cgccccgtcg
     6241 ctgcatcacc tcgagggcaa gatggccgac tgtttcctgg ccggtgccat cgcgacgggc
     6301 tgcgactacg aggtcgagga gaccgagccg ccgtatgacg aactggcacc ggacaaatgg
     6361 ctggccggag tcttccgcga cgagatgatt cgactgggcc gcgcccccgt aggcgaggag
     6421 atcgaggcgg ccctgccgct gggaagcacc gacatgggca acgtcacgca gctgatgccg
     6481 ggcatccacc ccgtcgtcgg cgtggacgcg gaaggcgcat cggtgcatca gccgggtttc
     6541 gcgacggccg cggcgagcgc gagtggcgac gaggccgtcg tcgagggagc catcatgctg
     6601 gcgcggacgg tcgtcgccct tgccgaaacg ccgggcgaac gggaccgggt gctcgagtcg
     6661 caggccaggc gggtggcgtc atgacgctcg ccggagccgc cgatagctgg ctggccgacc
     6721 actacgacga cgtggtggcc tggcgccgac acatccacat gcaccccgaa ctcggccggc
     6781 aggagttcgc caccacccag t
//
LOCUS       DASYLX010000432         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1335799,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000432 DASYLX010000000
VERSION     DASYLX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335799"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VGP27_10640"
     CDS             <1..535
                     /locus_tag="VGP27_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV7851798.1"
                     /translation="TVGVGQSEVAVANMVDTFVFLTLARTGDQLQGIKKGVLELADIV
                     VVNKADGNHAAEAKAAARDLTGAIRLIYPRETLWRPPVLTMSALEGSGLTDLWDTVLK
                     HRRVLTDAGEFDARRNSQQVDWTWSMVRDTVLDRVLNNPAVRGVRADVERQVRDGELT
                     PALAAQQILDAAESTKE"
     gene            1141..2415
                     /locus_tag="VGP27_10645"
     CDS             1141..2415
                     /locus_tag="VGP27_10645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7851799.1"
                     /translation="MRHSDSRERSAALPHGVQGAADPHFSCAVRAFSSMFPGPRYGGG
                     ALSVFLDGEPVVDVWTGWADRRGTTHWSADTGAMVFSATKGMASTVIHRLADRGLIDY
                     DAPVAEYWPEFGANGKAKITVRELMRHRAGLSQLNGATKADLMDHLKMEERLAAAPVN
                     RVLYGKGAYHALTYGWLMSGLARSVTGIGMRQLIRTEVAEPLGTDGLHLGRPPAEAPT
                     KPAQIIIPQGTIKNAVFGALVPKLAALQWSGGIGALYFPEMVSVVQGDTPFLDGEIPA
                     ANGTATARGLAKMYAPLACGGRVDGRQFLSPELTAGLMGVPNLRPDRNIVFPMAFHLG
                     YHSIPLGGLMPGFGHAGLGGSLGWADPSTGMSFGYVHNRLLTPFVVADQAGFVATAAF
                     IRRGAAAARRRGYRPVPAFGAPFATEPRPVAG"
     gene            complement(2420..>3358)
                     /locus_tag="VGP27_10650"
     CDS             complement(2420..>3358)
                     /locus_tag="VGP27_10650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7851800.1"
                     /translation="RERRLTFVVVGAGPTGVELAGQIAELAERTLAGAFRTIKPSECR
                     VVLVEGAGAVLPPMGPKLGAKAQRTLEKMGVEIQLNALVTDVDYLGVAVKDKDGTERR
                     IECACKVWSAGVQASPLGKMIAEQSDGTEIDRAGRVIVEPDLSVKGHPNVFVVGDLMS
                     VPGVPGMAQGAIQGAKYVTKLIKHSVAGHDDPSTRAPFKYFDKGSMATVSRFSAVAKV
                     GKLEFSGFIAWLAWLALHLVYLVGFKNRFTTLITWFITFMSRSRGQMAITSQMIYARL
                     AMDMLQSQNDDLSRQARHSALAAVEEAEKLERERGA"
BASE COUNT          560 a         1119 c         1110 g          569 t
ORIGIN      
        1 aaccgtcggc gtcgggcagt ccgaggtggc ggtcgccaac atggtggaca cgttcgtgtt
       61 cctgaccctc gcccgcaccg gtgatcagct ccaaggcatc aagaagggtg tgctggagct
      121 tgctgacatc gtcgtggtga acaaggccga tggaaatcac gccgccgagg caaaggcggc
      181 cgcgcgcgac ctgacgggtg ccatccggtt gatctatccg cgcgaaacac tctggcgccc
      241 accggttctg acgatgagcg cgttggaggg ctccgggctg accgacctgt gggacaccgt
      301 gctcaaacac cgccgggtgc tcaccgacgc gggggagttc gacgcccgtc gtaactccca
      361 gcaggtggac tggacgtggt cgatggtccg cgacaccgtc ttggaccggg tgctcaacaa
      421 ccccgcggtg cgcggtgtcc gcgccgacgt ggagcgccag gtccgtgacg gcgagttgac
      481 acctgccctc gccgcgcagc agatcctcga cgcggcggag agcacgaaag aatagggaaa
      541 caccggatat tcaagcggtc gagctgatgg atagcgtcga ttacatgaat cgaagttccg
      601 tcagttctgt gttcgccggg ctggcgatct ccggcatgct gctggccccg gggaccgcaa
      661 gcgctctgcc gccgctgccc ggcgacaagg acgccgacgg cgtgtccgat aaagacgaga
      721 tcaacgtcta tcactcccac ccgaacaacc gcgcaccgac ggcgatcggg tcaacgacgg
      781 caacggcagg aggtcgacgc gggcaccaag cctctcgacc atgcgtcgca tctgggggcg
      841 gccaacgacc aggctcctca aggccaacag gcgaaccgct ccgacaccga caacgacggg
      901 ctgttcaatg atgacgagat caacgtctac ggcacgaacc cgaacaaggc ggacaccgac
      961 ggcgacggcc gcgacgacgg ccaggaggtc ttcgacaaga ccgaccccaa taacccgttc
     1021 gactgaactg gcagcgatga ttgctgttcg gtgcagcctc agcccctctg acatgggagg
     1081 cgttaacgga tcggtaacgg tgcgtttatt tgccggggtt gcaggtaaat tagatgcatt
     1141 gtgaggcact ctgacagccg ggagcgcagc gcggcgcttc cccacggtgt tcaaggcgcg
     1201 gccgacccgc acttctcctg tgcggtacgg gccttcagca gcatgtttcc cggtccccgc
     1261 tacggcggag gggcgctttc ggtgtttctg gacggcgagc ctgtcgtcga tgtctggacc
     1321 gggtgggccg accggcgcgg caccacgcac tggtccgccg acaccggcgc aatggtcttc
     1381 tccgccacca agggcatggc ctcgaccgtc atccaccgac tcgccgatcg cggcctgatc
     1441 gactacgacg ccccggtcgc cgagtactgg ccggagttcg gcgcaaacgg caaggcgaag
     1501 atcaccgttc gcgagttgat gcggcaccgt gccgggctgt cgcaactcaa cggcgcgacc
     1561 aaggccgacc tcatggacca cctcaagatg gaggagcggc ttgcggccgc gccggtcaac
     1621 cgggttctct acggcaaggg tgcctaccac gcgctgacat acggctggct gatgtctggt
     1681 ctggcgaggt cggtcaccgg catcggcatg cggcagctga tccggaccga agtcgcggag
     1741 ccgcttggca ccgacggtct gcatctgggc cgcccgcccg ccgaggcgcc gacgaagccg
     1801 gcgcagatca tcattccgca gggcaccatc aagaacgcgg tgttcggtgc gctggtaccg
     1861 aagctggcgg cgctgcaatg gtccggcggc atcggcgcgc tgtactttcc ggagatggtt
     1921 tccgtagtgc agggtgatac cccgttcctc gacggcgaga ttcccgccgc caacggaacc
     1981 gcgaccgcgc ggggcctggc gaagatgtac gcgccgctgg catgcggcgg gcgcgtcgac
     2041 ggcaggcagt tcctgtcgcc cgagctgacc gcaggcctga tgggtgtgcc caacctgcgt
     2101 ccggaccgaa acatcgtgtt ccccatggcg tttcatctcg gttaccactc catcccgctt
     2161 ggcgggttga tgccggggtt cggccacgcc ggtctcggcg gttcgctcgg gtgggcggac
     2221 ccgagcaccg ggatgtcctt cggctatgtg cacaaccggc tgctgacgcc gttcgtggtg
     2281 gccgaccagg cgggcttcgt cgcgacggcg gcgttcattc gccggggcgc ggcggcggcg
     2341 cgcaggcggg gataccggcc ggtgcctgcg ttcggcgcgc cgttcgctac cgagccgcga
     2401 cccgtcgccg gctagggatt tacgcgcctc tttcccgctc gagcttttcg gcctcctcga
     2461 cggcggccag cgccgaatgc cgggcctgcc ggctgaggtc gtcattttgg ctctgcagca
     2521 tgtccatggc caaccttgca tagatcatct ggctggtgat cgccatctgt ccgcggctgc
     2581 ggctcatgaa cgtgatgaac caggtgatca acgtggtgaa ccggttcttg aagccgacca
     2641 gatacaccag gtgcagcgcc agccacgcga gccaggcgat gaatccgctg aattcgagct
     2701 tgccgacttt tgccacggcg ctgaaccggg acacagtcgc catgctgccc ttgtcgaagt
     2761 acttgaacgg ggcgcgggtg gaggggtcgt cgtggccggc caccgagtgc ttgatcagtt
     2821 tggtcacata cttggcgcct tgtatcgcgc cctgcgccat tccaggcacg ccgggcaccg
     2881 acatcaggtc gccgacgacg aagacgttcg ggtgaccctt cacgctgagg tcgggctcga
     2941 cgatcacccg gccggcgcgg tcgatctcgg tgccgtcgga ctgctcggcg atcatcttgc
     3001 cgagcgggct ggcctgcaca cccgccgacc acaccttgca cgcgcattcg atgcggcgtt
     3061 cagtgccgtc cttgtccttc accgccacgc ccagatagtc gacgtcggtc accagcgcgt
     3121 tcagctggat ctcgacgccc atcttctcca gcgtgcgctg cgccttcgcg ccgagcttgg
     3181 gacccatcgg cggcaatacg gccccggctc cctcgaccag gacgacccgg cactcgctcg
     3241 gcttgatggt gcggaaagcc cctgcgagtg tgcgctcggc gagttcggcg atctgaccgg
     3301 ccaactccac gccggtcggt ccggcaccga cgaccacgaa cgtcagccgc cgttcacg
//
LOCUS       DASYLX010000433         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2761707,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000433 DASYLX010000000
VERSION     DASYLX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2761707"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="dop"
                     /locus_tag="VGP27_10655"
     CDS             complement(<1..1216)
                     /gene="dop"
                     /locus_tag="VGP27_10655"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEV7851801.1"
                     /translation="MQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAK
                     RTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYS
                     APECTDPLDAVVWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYL
                     MSRQTPFSAVIAGLTPHLVSRQVITGSGRVGIGPSGDEPGFQLSQRSDYIEVEVGLET
                     TLKRGIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKVGATALVLDLIEEGMDLT
                     DLALARPVHAVHVISRDPSLRATVALADGRELTALALQRIYLDRVAKLVDSRDPDPRA
                     ADVVQTWANVLDLLERDPMECAELLDWPAKLRLLEGFRNRENLNWSAPRLHLVDLQYS
                     DVRLDKGLYNRLV"
     gene            1522..2676
                     /locus_tag="VGP27_10660"
     CDS             1522..2676
                     /locus_tag="VGP27_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851802.1"
                     /translation="MVSAEVAQRRAADRWFLERGLPAVLRPGALVRRLIPRSAPAMAT
                     LAVFMANSVLVVLVSGKHTIDIDGHPTRTEWFVLALLVLVLPIAVTVGWLVSRIRSVR
                     VRTLVAVGSVGVAVAGGILGGPSPRIVTNLVFEGIVILAALAFTACGAGSILGWGLRT
                     ALANLAFVGELFVRALPVVLLTVLVFFNTYVWSMASTISRPRLWLALSFLGLIAIAFV
                     TSATVDRVRPMLAGSRQSFDDDIRLTGTPFEGMPHPAAGYPLSWTERCNVIFVLATSQ
                     VLQVGLVAIVAGLIFFVLGLILLSPQLLAQWTRNGPSDGQLLGMTLPVPQALIQISMF
                     LAAMTFMYVSARAVGDGDYRTRFLDPLIDDLRLTLVARSRYRAAVAGTGS"
     gene            complement(2750..3379)
                     /locus_tag="VGP27_10665"
                     /pseudo
     CDS             complement(2750..3379)
                     /locus_tag="VGP27_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3512..>3814)
                     /locus_tag="VGP27_10670"
     CDS             complement(3512..>3814)
                     /locus_tag="VGP27_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEV7851803.1"
                     /translation="FKDFNSGAMIQNVVDRAKKNAIKAVLETGQPGLRIQHLLDSIVD
                     EFAENEDLPNTTNPDDWARISGKKGERIVYIRTLVTGKSSSASRAIDTESNLGQYL"
BASE COUNT          567 a         1241 c         1268 g          738 t
ORIGIN      
        1 ccaccagccg gttgtagagc cccttgtcga ggcgcacatc ggagtactgc agatcgacca
       61 ggtgcaggcg cggtgccgac cagttgaggt tctcccggtt gcggaagcct tcgagcagac
      121 ggagtttcgc gggccagtcc aacaactcgg cgcactccat cgggtcgcgc tcgagaaggt
      181 cgagcacgtt cgcccaggtt tgcacgacgt ccgcggcgcg tgggtcggga tcgcggctgt
      241 ccaccagctt ggccacccgg tccaggtaaa tccgttgtag cgcaagggcg gtcagctcgc
      301 ggccatcggc caatgcgacg gtggcgcgca gcgatgggtc gcggctgatc acatgcacgg
      361 cgtgcaccgg ccgggccagc gccaggtcgg tgaggtccat gccctcttcg atcaggtcca
      421 gcaccagcgc ggtggcaccc accttgagat acgtagacgt ctcggcgagg ttcgcatcgc
      481 cgatgatgac gtgcagccgc cggtacttgt cggcgtcggc gtgcggctcg tcgcgggtgt
      541 tgatgatgcc gcgcttgagc gtcgtctcca ggccgacctc gacctcgatg tagtcggagc
      601 gctgcgacag ctggaatccg ggctcgtcgc cggaggggcc gatgccgacc cggccggagc
      661 cggtgatcac ctggcgcgac accagatgcg gcgtcagacc cgcgatcacc gcggagaacg
      721 gcgtctgacg cgacatcaga tagttctcgt gcgaaccgta ggacgcgccc ttgccgtcca
      781 cgttgttctt gtagagctgc agcttcgccg cgcccggcac gctggccaca tggcgtgcgg
      841 cggcctccat cacccgttcg ccggctttgt cccagacgac cgcgtccagc gggtccgtgc
      901 actcgggtgc cgagtactcc gggtgggcgt ggtcgacgta cagccgcgcg ccattggtca
      961 ggatcatgtt ggcggcaccg acctcgtcgg cgtccaccac cggcggcgga cctgccgacc
     1021 ggctcaggtc gaaaccacgc gcgtcgcgca gcggcgattc cacctcgtag tcccaccggg
     1081 tgcgcttcgc gcgctgaatc ccggcggcgg ccgcatacgc cagcaccgcc tgcgtcgacg
     1141 tcaggatcgg attcgcggtc gggtccgacg gcgaagatat gccgtactcg acctctgttc
     1201 cgataatccg ctgcatatcc ccagcctagg cgacgacccg aagtagcctg cgaccgtgcc
     1261 atcggatcag cacgatcgcg acacgaccgc gagccggttg gtggccacgc tgggcgccac
     1321 tatggccgcc gcgaatcatc cggtcagcat cgttcgcgag gtgatggctg cggatcgccc
     1381 agcttgcgtt cggcatcctg gtcgccgcgc aggtgggcgg gggtcacgga gggcgacttc
     1441 gtcggcttca tgggtgttac cgaactgacc gaaacgccgc accggcgtgc gaggcatgat
     1501 caatgacggg agtacgcacc gatggtgtct gcggaggtcg cacaacggcg cgctgccgat
     1561 cgatggttcc tcgaacgtgg gttgcctgcg gtacttcggc ctggggcgtt ggtgcgacgg
     1621 ctgatcccac ggtcggcacc cgcaatggcg actctggcgg tcttcatggc caactccgtc
     1681 ctggttgttc tcgtcagcgg caagcacacc atcgatatcg acggtcaccc gacgcgcaca
     1741 gagtggttcg tcctggcgct gctggtgctg gtcctgccga tcgcggtgac cgtcggctgg
     1801 ctggtgtcgc ggatccgtag cgtgcgcgtc cgcacgttgg tggccgtcgg gtcggttggc
     1861 gtagccgtcg ccgggggcat cctcggcggc ccgagcccgc gaatcgtcac caatcttgtg
     1921 ttcgagggca tcgtgatcct ggctgcgctg gcattcacgg cgtgcggagc ggggtcgatc
     1981 ctggggtggg gcctgcggac cgccttggcg aatctcgcat tcgtcggcga gctgttcgtg
     2041 cgcgcgctgc ccgtcgtgct gctcaccgtc ctggtcttct tcaacaccta cgtctggtcg
     2101 atggcgtcga caatcagccg gcctcggctg tggctggcgc tgtcctttct cggcctgatc
     2161 gccatcgcgt tcgtcacatc ggcgacggtt gaccgagtgc ggcccatgct ggcggggtcc
     2221 cgccaatctt tcgacgacga catccggttg accggcaccc ctttcgaggg catgcctcac
     2281 cccgccgccg gctatccgtt gtcatggacg gaacgctgca atgtgatctt cgtgctcgcc
     2341 acgtcgcagg tgcttcaggt cggtcttgtg gccatcgtcg ccgggctcat ctttttcgtg
     2401 ctgggtttga tcctgctcag tccgcagttg ctggcgcagt ggacacgaaa cggcccgagt
     2461 gacggccagc tcttggggat gacgctgcct gtgccgcagg ccctgattca gatcagcatg
     2521 ttcctcgcag cgatgacgtt catgtatgtc agcgcacggg ccgtcggcga cggcgactac
     2581 cggaccaggt tcctcgaccc gctcatcgac gatcttcggc tgacgctggt cgcgcgcagc
     2641 cgctatcggg ccgccgtcgc aggcaccggt tcgtaaccct tttcgtcacc gcgagcgtgc
     2701 gtgtctgcac cagacacgcc gctcagcggc gtcactctgt gcacgctcgt cacgaaacga
     2761 actgcaggca atagctattc gcgagatcgt cctgggtcac acaagcgggc tgccctgtgg
     2821 tttcgacaac gagcggagta ctcggggtgc gagtctggta gctccagccc tggggcagct
     2881 tcagccggct tgcaaggcct ggcagatcgg ccagcgtcag agccgggtcg atcgtctggc
     2941 tgaatgtctg cataacccag cgcctgccgt cggggtcgac gagctcatag atctcccggc
     3001 ccgcgtcgaa cgtgaatacc gcttttcggt caacctggtt gacgttatac ggcgccgggt
     3061 tcatggaggc cagccctacg gtggcctgcc ggatcatctc gaggccaccg aaagtgctgt
     3121 gttccagtgc ttctcgatgt agcttgccga tgctgctcat cagccagtac ctgggtccgt
     3181 tcagcagggc ggcgacggtg ccgttccttt ccgcgatgac ctccgcgttc aacgcgtccc
     3241 acagttcggc ggggcagtcg ttgagcggga aagtgtttta gacggtggcc tgtggacctg
     3301 actcgccgac tgtgaccaga agcacttcgc cgtagcgctt gccgaatacg ccgtcgatca
     3361 cagagatgtc atcggacata tcgcgacggt agccctcggc acgccgcgaa ggcgcgcata
     3421 gtgccgcgat tcgacggcgt gtcctgctgc agacacgcac gctcgcgggg cgggggtgac
     3481 cgaccccggc tcgcggtgcg aggccttagg cctacaggta ctgacccagg ttcgactcgg
     3541 tgtcgatcgc ccgcgaggcg ctcgacgact tgccggtgac cagggtgcgg atgtagacga
     3601 tccgctcgcc cttcttgccc gagatccggg cccagtcatc gggattggtg gtgttgggca
     3661 ggtcctcgtt ctcggcgaac tcgtcgacga tcgagtcgag cagatgctga atgcgcaagc
     3721 cgggttggcc cgtctccagc accgccttga tcgcattctt cttcgcgcgg tcgaccacgt
     3781 tctggatcat cgccccggag ttgaagtcct tgaa
//
LOCUS       DASYLX010000434         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1335867,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000434 DASYLX010000000
VERSION     DASYLX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335867"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..895
                     /locus_tag="VGP27_10675"
     CDS             182..895
                     /locus_tag="VGP27_10675"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEV7851804.1"
                     /translation="MQLTVLSGAGISAESGVPTFRDVETGLWAKVDPYEISSAQGWQR
                     HPEKVWAWYLWRHYMMQAVEPNNGHRAVAAWQDHATVHVVTQNVDNLHERAGSQRVYH
                     LHGSLFEFHCDRCQREYTGALPEMPEPVDAVAPPECQCGGLIRPNVVWFGEALPDDAW
                     QKSVDAVSGADAVIVVGTSSIVYPAAGLPELALAKGIPVIEVNPERTPLSDAATATVR
                     EPAATALPTLLQRLPLLLG"
     gene            complement(946..1599)
                     /locus_tag="VGP27_10680"
     CDS             complement(946..1599)
                     /locus_tag="VGP27_10680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7851805.1"
                     /translation="MTDAGDTTDNPFFARLWTFLSNHETEEVQRLRTENLAGLTGRVL
                     EVGAGTGTNFGYYPATVTEVVAVEPERRLAPQARKAAAKAPVPVTVSTGTVERFLSDG
                     DGKPFDAVVCSLVLCSIDDPDSVLRQLFSLVRPGGELRFLEHVGSTGLRGRLQKVADA
                     TVWPRLMGNCHTHRDTEASILGAGFAVQDARREPAMPAWFPTPVAEYTIGRAVRPVA"
     gene            complement(1596..2294)
                     /locus_tag="VGP27_10685"
     CDS             complement(1596..2294)
                     /locus_tag="VGP27_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851806.1"
                     /translation="MSLQPLKRRRAPRGSGEQLRNEILDATTQLLLETGHAKAVSIRS
                     VAQRVGVTPPSIYLHFADKDTLLDAVCARYFEKLDEEMQRAALDEPSTVEVLRAQGLA
                     YVRFAMQTPELYRIATMGEGRPGSDIDMTLNSSAFQHLRSSVEKLIEEGVYPPGDSTM
                     AALQLLTAAHGVASLLISKPYLPWGDVEEFADQTLSAACLGLIVLGLVEDDDATPQQT
                     VDWLLNRKPREGKS"
BASE COUNT          376 a          783 c          756 g          399 t
ORIGIN      
        1 gccgcgagct cgcgcaccgt cggcagccgc gtgcccggtg gcagccggcc atttcggatc
       61 ccgtcgatga tctgactcct gagctggtcg aacaacggcc tgccggcccg cggatcagcc
      121 tgaacccatt cccccaaatc ggtcacgtgc tcagtatcgc gcatggcgtt actttggtga
      181 ggtgcaattg acggtgctca gcggagcagg tatctccgcc gagagcgggg tcccgacgtt
      241 tcgcgacgtc gaaacgggtc tgtgggccaa ggtcgacccg tacgagatct cgagtgcaca
      301 gggctggcag cggcatccgg agaaggtctg ggcgtggtac ctgtggcggc actacatgat
      361 gcaggccgtc gaaccgaaca acggtcaccg ggcggttgcg gcgtggcagg atcacgccac
      421 cgtgcacgtc gtcacccaga atgtcgataa cctgcatgaa cgcgccggca gtcagcgggt
      481 ctaccacctg cacggcagcc tgttcgaatt ccattgcgac cgttgtcaac gcgagtacac
      541 cggggccctg cccgagatgc ccgaacccgt cgatgcggtg gcgccgccgg aatgccagtg
      601 cggcggcctg atcaggccca atgtggtgtg gttcggcgaa gcgttgcccg atgacgcctg
      661 gcagaagtcg gtggatgcgg tcagcggtgc cgacgcggtc atcgtggtgg gcacgtcgtc
      721 gatcgtctac cccgccgcag gcctccccga actggcgctg gccaagggca ttccggtgat
      781 cgaggtcaat cccgagcgca caccgctgtc tgacgccgcg acggccacgg ttcgggagcc
      841 cgctgcgacc gctctgccga cgctgctgca gcgcctcccc ttgcttctcg gctaattgcg
      901 acttcggtgc atcgacgctg agcgcacgct agcgcgccga aatcgctagg cgacgggcct
      961 gaccgcgcgg ccgatggtgt actccgcgac cggtgtcggg aaccacgccg gcatggccgg
     1021 ctcgcgacgc gcgtcctgca ccgcgaagcc cgcgccgagg atcgacgctt cggtgtcgcg
     1081 atgggtgtgg caattgccca tcaggcgcgg ccacaccgtt gcgtcggcga ccttctgcag
     1141 ccgtccgcgc aacccggtgc ttccgacgtg ctcgaggaag cgcagctcgc cgccgggccg
     1201 gacgagcgag aaaagttgtc gcagaacact gtccgggtcg tcgatcgagc agagcaccag
     1261 agagcacacg acggcgtcga acggcttccc gtcgccatca ctgaggaacc tctcgacggt
     1321 gccggtgctc accgtgaccg ggacgggcgc tttcgccgcc gcttttcgcg cctgcggcgc
     1381 cagccgtcgt tcgggttcga cggcgacgac ttcggtcacg gtcgccgggt agtacccgaa
     1441 gttcgttccg gtacctgcgc cgacttccag cacgcggccg gtcagaccgg ccaggttctc
     1501 cgtccgtagc cgctggacct cctcggtttc gtggttcgac agaaaggtcc acagtcgggc
     1561 gaagaacgga ttgtctgtgg tgtcgccggc gtcggtcacg acttcccctc ccgcggttta
     1621 cgattcagca accagtcgac cgtctgctgt ggtgtcgcat cgtcgtcctc gacgagcccg
     1681 agcacaatta ggcccaaaca cgccgccgac aacgtctggt cggcgaattc ctccacgtcg
     1741 ccccacggca gatacggctt cgaaatcagc agtgaggcaa cgccgtgagc ggcggtcagc
     1801 agctgcaacg cggccatcgt cgaatcaccg ggcgggtaga caccctcctc gatcaacttc
     1861 tcgaccgagc ttcgcaggtg ctgaaacgcc gagctgttca gtgtcatgtc gatgtcgctg
     1921 ccgggcctgc cctcacccat cgtggcgatg cggtacaact cgggcgtctg catcgcgaac
     1981 cggacatacg ccaggccctg cgcccgcagc acctcgaccg tcgacggctc gtcgagggcg
     2041 gcccgctgca tctcctcgtc gagcttctcg aagtaccgcg cacagaccgc gtccagcagc
     2101 gtgtccttgt cggcgaagtg caggtagatc gatggcggtg tgacgccgac gcgttgcgcg
     2161 accgaccgga tggacaccgc cttggcgtgc ccggtctcca gcagcaactg ggtggtggcg
     2221 tccaggattt cgttcctcag ctgctcgccg gagccacgcg gcgcacgccg tcgcttgagg
     2281 ggctgtagag acatcgtcaa ccagtgtccg tcac
//
LOCUS       DASYLX010000435         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1335877,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000435 DASYLX010000000
VERSION     DASYLX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335877"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VGP27_10690"
     CDS             <1..947
                     /locus_tag="VGP27_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7851807.1"
                     /translation="VGDLVSVPFNIACGRCVNCKERKTGICLNVNPDRPGSAYGYVDM
                     GGWVGGQAEYVMVPYADFNLLKFPDADQAMEKILDLTMLTDIFPTGFHGAVTAGVGAG
                     STVYIAGAGPVGLATAVSARFLGAAVVIVADMNEERLAQARSFGCETVDVSKGSPQGQ
                     IEQILGVPEVDCAVDAVGFEAKGHGADAGRDAPATVLNSLMDITAAGGGIGIPGLYVT
                     GDPGGVDDAAKRGALSLSLGTGWAKSLSFTTGQCPVMQYHRGLMQAILRDRVQIAKAV
                     GATVITLDEAPHGYAEFDSGVSKKFVIDPHGELASQAA"
     gene            2114..2401
                     /locus_tag="VGP27_10695"
     CDS             2114..2401
                     /locus_tag="VGP27_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851808.1"
                     /translation="MRVGLKYITPVLAAGAAAVAIAAAPAAMAETTPAQPAMIASAPA
                     AVAPNIVQTAGHGGGGFQGGGFHGGGDRGGWHGDRGWHGDNGWGNWFGWRR"
     gene            complement(2602..2919)
                     /locus_tag="VGP27_10700"
     CDS             complement(2602..2919)
                     /locus_tag="VGP27_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851809.1"
                     /translation="MSDVTSGSSRYMPCVSAHGALKIRHYRIDGEPPRQPREHPRTRA
                     TAPRLRHGLAQFDQFARPVLGVAPLDRCQLVQYFLSLAVRGFREAVIQVGTVNRPGES
                     GDG"
     gene            2977..3543
                     /locus_tag="VGP27_10705"
                     /pseudo
     CDS             2977..3543
                     /locus_tag="VGP27_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
     gene            complement(3581..4054)
                     /locus_tag="VGP27_10710"
     CDS             complement(3581..4054)
                     /locus_tag="VGP27_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEV7851810.1"
                     /translation="MTTVNGLPSHVLFVHFIVVLAPLTALLAILCAVWPAARRRLVWL
                     VLGLAVVNAALTPITTHAGEWLENRVGRSPRLHTHTELGDTMLYFSIALLVAAILLAL
                     LHVRVSRAGSVNTVAAVAIAVVVVVASVATTVQVYRIGDSGAQAAWGDTAAASIP"
     gene            4321..4638
                     /locus_tag="VGP27_10715"
                     /pseudo
     CDS             4321..4638
                     /locus_tag="VGP27_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872444.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
BASE COUNT          822 a         1433 c         1593 g          790 t
ORIGIN      
        1 ccgtcggcga cctggtgtcg gtccccttca acatcgcctg tggtcgctgc gtgaactgca
       61 aagaacgcaa gaccggcatc tgtctcaacg tcaatccaga ccggcccgga tcggcttacg
      121 gatacgtaga catgggcggc tgggtcggcg gccaggccga atatgtgatg gtgccctacg
      181 ccgacttcaa cctgctgaaa ttccctgacg ccgatcaggc catggagaag atcctcgatc
      241 tgacgatgct cacggacatc ttcccgaccg gattccacgg ggccgtcacc gccggtgtcg
      301 gagcaggttc cacggtctac atcgccggcg ccggccccgt cgggctcgct actgccgtca
      361 gcgcacgatt cctcggcgcc gcggtggtca tcgtcgccga catgaacgag gaacggctcg
      421 cgcaagcgcg gtccttcgga tgcgagaccg tcgacgtctc caagggttcg cctcagggcc
      481 agatcgagca gattctcggc gtcccggagg tcgattgcgc agtcgacgca gtcggtttcg
      541 aagccaaggg gcacggcgcc gacgcaggtc gtgatgctcc cgcgacggtg ttgaactcgt
      601 tgatggacat caccgcggcg ggcggcggga tcggcattcc cggcctgtat gtcacgggtg
      661 atccgggcgg tgtcgacgac gccgccaagc gcggtgcact ttcgctgagt ctgggtactg
      721 gatgggccaa gtcactgtcc ttcaccacgg ggcagtgccc ggtcatgcaa taccaccgag
      781 gtctgatgca ggccatcctg cgtgaccgag tgcagatcgc gaaggctgtc ggtgccaccg
      841 tcatcaccct ggacgaggcg ccgcacggct acgcggagtt cgacagcggt gtttccaaaa
      901 agttcgttat cgacccgcat ggagaactcg cgtcgcaagc cgcctagagt aagcacttca
      961 cttcgaaacg gtgccgagta tgccgagacc atagacggcg ctgcggtgcc gcagatcggc
     1021 tgactgactt tccctgcggg ggtaaacact tgtagtaccg cgccgccatg tcgaagacgt
     1081 tatccggacc tcacacacga ccagtacctc gctcttcaga ccgtcgtata cagagttgta
     1141 cgcgcaatca acgaggtcct cccaactgaa cgaacctacg ttgtgtcatt gatgggaccc
     1201 ctggaatttg tccagtggtg agagtcgatt tgttctggtt acagaaatcg gcccgccacc
     1261 cgtgtcgatc aggttcacgt tggagacacg cactcgcgcc gggccgtcta ggcatgtgca
     1321 ctgagtgttc aggggtgggt ggttccgacg agctgaccct gcatcgcgaa cagcttcctg
     1381 cgctaaccgc gccacgactc ggaccaccac tgagggatca gcctcaggcg atcggcttga
     1441 ggcgacgccc agcgacttat acgatcctga ccaggttaat acgaagccgc cgagggcatc
     1501 tcgtggggcg ttgaagtagt catgaaacga ggaatcatag ggtttgcaac gacgatgttg
     1561 gtgtgcggag gtttcgggct ggcgtcgggt accgcccaag ccgagcccgg ctttggccct
     1621 gcgccgctgt accactggta cccgggggaa ttctggcgcc cggaatgggg cttcaacttc
     1681 gactcatact tgccacgacg atcaccaccg cgatggcgac ggcgacggcg acaaccacaa
     1741 caacgatttt cggcggtagg tctgcgccgc tgatcggcgc ccgtcggtgc tgaacccgtc
     1801 gggccggccc tggtttggtg ttccatcgcc gcccctaacg ccgcggtcgg cccgccaccc
     1861 cttggggatc ttggatggcg ggccgaggcg gaagacgttc agactgcggg gtattggccg
     1921 tagaagccgc gtgggtcgcg catcattgtg ccccttccat ctgggaatca cctgcagcgc
     1981 cgctggctgc tgacaggtaa agcaaggatg ccagttggta gggggcatgg atgggggtca
     2041 gcggtgacga cgtgggttcg ctggagcgca attctgggat gagtccagat tggaccacca
     2101 aaggagtagc accatgcgag tcggactgaa atacatcaca ccagtgttgg cggcgggcgc
     2161 tgccgcggtg gctatcgccg ccgcaccagc ggccatggcc gagaccacgc cggctcagcc
     2221 ggcaatgatc gccagcgcgc cggcggccgt ggcccccaac atcgtccaga ccgccggtca
     2281 cggcggcggc ggcttccagg gcggcgggtt tcatggcggc ggtgaccgcg gcggctggca
     2341 cggtgaccgc ggctggcacg gcgataacgg ctggggtaac tggtttggct ggcgccggta
     2401 acggtcaacg agcttgcgct gaaggctgca tttagctgat accgctgctc gtaggtgaag
     2461 gatcgcctac ccaaggacag gcccgcgccg gtgacccctc gccggcgcgg gccgccggac
     2521 accgagccgc cgaactagaa cgtcatgtcg ggacgccacc caagcaggat gacgggccga
     2581 tatcaggagc gggagtctca actaaccgtc tccggactca ccggggcggt tcacagtgcc
     2641 gacttgtatg acggcctcgc ggaagccgcg cacggccagc gacaggaaat actgaaccaa
     2701 ctggcagcgg tcgagcggcg ccacgccgag cactggacgg gcaaactggt cgaactgggc
     2761 gagcccgtgc cggagccggg gagccgtggc tcgcgtacgc gggtgttctc gtggctggcg
     2821 cggcggttct ccatcgatgc ggtagtgccg tatcttgagc gcgccgtgcg cgctgacgca
     2881 gggcatgtac cgggacgacc ccgaggtcac gtcggacatg gccgtttccg aaacgttcac
     2941 acgcccgggt gctgacgcaa ctgcgcggtg gcgacagtga cgatcgggat gtgctgcgcg
     3001 ggagcgctgt cgatggccgc aggtgaatac atcccgatgc ggagccagcg cgaggcctat
     3061 caaggcgaga tcaccctgga ggaacaggaa ctgcgcgacg ccccgaggct gaggccgagg
     3121 agctggcgcg gatctaccgc gccaaagggc tggacgcaga ggaggccgag agcgtcgcgc
     3181 gaccatcatg aaagaccgcg aggcggcgct cgacacgata gcgcgcgaag agcccaggct
     3241 tgatcccgac gaactcggct cgccgtggcc gccctgtcca gtctcggtgc gttcgcggcg
     3301 ggagaggttg tggtcgtcct gccgtacatg ttctcgtcgg gctggtcggc gctgatcacc
     3361 gcgatcgtct tggcgagcct cgcctcgttc gccgtcgggg cggtgatcgg tgtcctaaac
     3421 ggccgcggcg gcctgcgatc ggggatgcga cagttgctga ttggcggcgg cgcggcactg
     3481 ctcgtctact tgatcggtca cggcgtcggc gcccttaccg gcgtccagct cggcggtggg
     3541 taacaccgcg cccccgtcaa cggtgttgca gcagaacgcg tcaaggtatg gacgcggctg
     3601 cggtgtcgcc ccacgccgcc tgcgcccccg agtcgccgat gcggtagacc tgcaccgtgg
     3661 tggcgacgct cgcgacgacg accacaaccg cgatggcaac cgcggcaacg gtattcaccg
     3721 aaccggcccg gctgacccgc acatgcagca gcgctagcag tatcgcggcg acgagtaggg
     3781 caatcgagaa gtagagcatc gtgtcgccga gttcggtgtg cgtgtgcagt cgtggtgagc
     3841 gaccgacacg gttctcgagc cattcaccgg cgtgggtggt gatgggagtg agcgcagcgt
     3901 tgacgacggc gagtcccagc accagccaca ccaggcggcg ccgcgctgcg ggccaaacag
     3961 cgcacaggat ggccagcagc gcggtcaatg gggccaatac gacgatgaag tgcacgaaaa
     4021 gaacatggct cggcaggccg ttgacggttg tcatcgcctt cctccctctg gcggtgaaat
     4081 tccagcccga atgtactacc gattcttaag ttgaacctga acgtcctgcc gggtgcagcc
     4141 gacgaattgg caagtgcggt aaggcttagc tggctagcgg gacggtccag ggtcgggttg
     4201 ggagtcgtag attgctgacg gtacgcaatt tccgccgcgg ccggattttt accgggcggc
     4261 actggccacg cggctcgaaa ccgcgattcg cgcgacgccg tcgaacccca cagcgcgggg
     4321 ttgtccagtc ggcctagctg gcttcgggcg gcggtgctgg gcgcgaacga cggaatcgtg
     4381 tccgtcgggg gccaccacag cgcgtggcgc cgaattcacc gctggcctgg cgggcctggt
     4441 ggccggcgcg gtgtcgatgg cgctccggcg aatatgtgtc ggtgtcgagt cagccagaca
     4501 gcgacaagtc gctgctcgcc cgggaccggc gagagttgcg gtagatgccg gctgcggagt
     4561 tggacgaact ggcggcgata tacgaggcca agggcgtctc ttcgcggacc gcggcgcaag
     4621 tcgccgccga gttgaccg
//
LOCUS       DASYLX010000436         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3640196,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000436 DASYLX010000000
VERSION     DASYLX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3640196"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..713
                     /locus_tag="VGP27_10720"
     CDS             156..713
                     /locus_tag="VGP27_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851811.1"
                     /translation="MNEPGRDQPGASETVDDTSTRHRILVATAEVLGRSGQTKLSLSE
                     VALQAGVSRPTLYRWFADKSELLDAFGVYEREMFDTGIGRATASLRGTEKLDAALRFI
                     VSYQQSYSGVRLVDIEPEVVITQLSRIIPIMRARLLKLLSGSNSGVKAATAIRVAVSH
                     YIVRSDDDDQFLAQLRHAVGIKPAS"
     gene            713..1141
                     /locus_tag="VGP27_10725"
     CDS             713..1141
                     /locus_tag="VGP27_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7851812.1"
                     /translation="MDGHVSDELEIVALLNRYARAVDTKDWELYRSVFTDDAHIDYST
                     DVFTGSLDGAIEFFSRDFSALVSMSMHYITNVETTIDGDTAEVRAMFYNPTQIKGLPD
                     LSMFGGYYYHDLIRTPAGWRSRSLREEMVWTANPPRPGQA"
     gene            complement(1132..>1837)
                     /gene="rlmN"
                     /locus_tag="VGP27_10730"
     CDS             complement(1132..>1837)
                     /gene="rlmN"
                     /locus_tag="VGP27_10730"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV7851813.1"
                     /translation="GLKRNLSTAEILEQVRAAAVELRDRDGDGIAPPARGGRLSNIVF
                     MGMGEPLANYNRVLAAVRRIVAAPPNGFGISARSVTVSTVGLAPAIRKLADEQLGVTL
                     ALSLHAPDDELRDTLVPVNSRWKVSDALEAARYYADVTGRRVSIEYALIRDVNDQPWR
                     ADLLGKRLHGALGPLVHVNVIPLNPTPGSEWDASPKAAEREFVRRVRGRGVSCTVRDT
                     RGREIAAACGQLAAQA"
BASE COUNT          340 a          611 c          581 g          305 t
ORIGIN      
        1 ctatgcttta cagatcgtga catcttgtaa cgcttgcgtc cgctacaggc caagactagg
       61 aggtccgcgg tggccgagac gacacggtcg cactcctgat gagctcgctg tgaggcagga
      121 tctacgatca ctaaacccga cgattgaggc ctgacgtgaa cgaaccaggg cgtgaccaac
      181 caggcgcgtc ggagaccgtc gacgacacct cgacgcgaca ccggatcctc gtggcgaccg
      241 cggaggtgct gggccgcagc ggccagacga aactcagcct gtcggaagtg gcgctgcagg
      301 ccggcgtctc ccgtcccacg ctgtaccgct ggttcgccga caagagcgag ctgctcgacg
      361 cgttcggcgt ctacgaacgc gaaatgttcg acaccggcat cggcagggcg accgcgagtt
      421 tgcgcggcac cgaaaagctg gacgcagcat tgcgattcat cgtctcctat cagcagtcct
      481 attcgggtgt gcggctcgtc gacatcgagc cggaggtcgt catcacgcag ttgtcgagaa
      541 tcatcccgat catgcgtgcc cggctgctga agctgctgtc aggatccaac agcggtgtca
      601 aggccgcaac ggcgatccga gtcgcagtgt cgcactacat cgttcgcagc gacgacgacg
      661 atcagtttct ggcccagttg cgtcacgccg tcggcatcaa accggcaagt tgatggatgg
      721 ccacgtcagc gacgaactcg agatcgtcgc gctgctgaac aggtatgcgc gggcggtgga
      781 caccaaggac tgggagctct accggtcggt gttcaccgac gacgcgcaca tcgactactc
      841 caccgacgtt ttcacgggat cactcgatgg cgccatcgag ttcttctcac gggatttctc
      901 ggcgctggtc tcgatgagca tgcattacat caccaacgtc gagacgacga tcgacggcga
      961 caccgccgaa gtccgcgcga tgttctacaa cccgacgcag atcaaggggc tccccgacct
     1021 cagcatgttc ggcggctact actaccacga tttgattcgc acgccggcag gatggcgcag
     1081 ccgaagtctg cgagaggaga tggtgtggac ggccaacccg ccgcgccccg gtcaggcctg
     1141 agcggccaat tgcccgcagg cggcggcgat ttcacggccg cgcgtgtcgc gcacggtgca
     1201 cgacacccca cgcccgcgga cccgccggac gaattcccgt tcggcggcct tggggctggc
     1261 gtcccactcg ctgccgggcg tcgggttcag cggtatgacg ttgacgtgga cgagcggacc
     1321 cagcgctccg tgcagccgct tgccgagcag gtctgcgcgc catggctgat cgttgacgtc
     1381 acgaatcagc gcgtattcga tcgacacccg cctgccggtc acatcggcgt agtagcgggc
     1441 ggcctccagc gcgtcggaga ccttccaccg gctgttcacc ggaaccaacg tgtcgcgcag
     1501 ctcgtcatcg ggtgcatgca gcgacagcgc cagcgtcaca ccgagctgct cgtcggccag
     1561 ctttcgaatc gccggggcca gtccgaccgt cgacacggtg accgaacgcg ccgagatacc
     1621 gaacccgttc ggcggcgccg cgacgatgcg acggaccgcg gcaagcacgc ggttgtaatt
     1681 ggccagcggc tcccccatgc ccatgaagac gatgttcgag agccggccac cgcgcgcggg
     1741 cggggcaatc ccatcgccgt cgcggtcgcg caactccact gccgccgcac ggacctgttc
     1801 gagaatctcg gccgtcgaca gattgcgctt cagcccg
//
LOCUS       DASYLX010000437         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1446027,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000437 DASYLX010000000
VERSION     DASYLX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446027"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VGP27_10735"
     CDS             <1..1604
                     /locus_tag="VGP27_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7851814.1"
                     /translation="EADHDLRNSTDMILLERVAKSVFHTDGIAQVQSITRPLGTPLDH
                     TSIPFQISAGSASQINNLPFQQARGADLLKQVDVINNSIDVLRQQYALQQQSGAATDE
                     QTKAFQQTVAVANDLRDKIADFDDFFRPLRNYFYWEPHCFDIPACAALRSLFDALDGI
                     GALTDQLASVSGSINKLDQLQPKLLALIPPQIASQQTNRDLTLTNYATTSGIDDQTAA
                     ALQNSTTLGQAYDASKTDDSFYLPPEAFTNPEFQRGLKLFLSPDGKAARMIITHEGIP
                     ATPEGISHIDAIRHAAQEAVKGTPLAGSKIFIAGTAATYKDIQDGAKYDLLIAGIAAL
                     SLILLVMMFITRSIVAAVVIVGTVALSLGASFGLSVLIWEDLLGIPLYWIVLALAVIL
                     LLAVGSDYNLLLISRFKEEIGAGLNTGIIRAMAGSGAVVTAAGLVFAATMASFVFADL
                     RILGQIGTTIALGLLFDTLIVRSFMTPSVAALLGRWFWWPLRVRTRPPGRRLQPSESG
                     RSGRQLRLWDDDDSAVADAPPPSQA"
     gene            complement(1665..2129)
                     /locus_tag="VGP27_10740"
     CDS             complement(1665..2129)
                     /locus_tag="VGP27_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851815.1"
                     /translation="MDLGVWGDLTIFAVVMEIVFASCVFVYMTRLERRTSHPLGEKVG
                     AHKTVLAKLRKREPMSQDEVDYAGELIADARSPLAYAIPAALFTIGFFYVVGCLYMLH
                     RDGGNPSFRTFIGGIPMLTSMNMAAQLRRVAGMKGKLRDVPAMAPEPAYHGR"
     gene            complement(2249..>2527)
                     /locus_tag="VGP27_10745"
     CDS             complement(2249..>2527)
                     /locus_tag="VGP27_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV7851816.1"
                     /translation="DHNAIRDAVRESRQHEAGTKKWFEAVFAARKENGEHLDEEEREA
                     MPDFIKSASLELRHELAMQWLRFYAEHETSKGIDNRDKDADAYIEHHS"
BASE COUNT          468 a          836 c          721 g          502 t
ORIGIN      
        1 tcgaagccga ccacgatctg cgaaattcga ctgacatgat ccttcttgag cgcgttgcca
       61 agtcggtgtt ccacaccgac ggcatcgctc aggtgcagtc gatcacccgg ccgctgggca
      121 cgccccttga ccacacgtcg ataccgtttc agatcagcgc gggcagcgcg tcccagatca
      181 acaacctgcc cttccagcag gcccgcggcg ccgacctgct caagcaggtc gacgtgatca
      241 acaattcgat cgacgtgctg cggcagcagt atgcgttgca acaacagtcc ggcgcagcca
      301 ccgacgagca gaccaaggca ttccagcaga cggtcgccgt ggccaacgac ctgcgcgaca
      361 agatcgccga cttcgacgac ttctttcggc ccttgcgcaa ttacttctat tgggagccac
      421 actgtttcga catcccggcc tgcgccgcgc tgagatcgct cttcgacgca ctcgacggga
      481 tcggcgcgct gacagaccag cttgccagcg tctcggggag catcaacaag ctggatcagc
      541 tgcagcccaa gctgcttgcg ctgattcccc ctcagatcgc gagccagcag accaaccgag
      601 acctgacgct gacgaactac gccaccacgt cgggcatcga cgatcagact gcggcggcac
      661 tgcagaactc caccactttg ggccaggcgt acgacgcgtc gaagaccgac gactcgttct
      721 acctcccgcc agaggcgttc accaaccctg aattccagcg cgggctgaag ctgtttctct
      781 caccggacgg caaagctgcg cgcatgatca tcacccacga agggattcct gcgactcccg
      841 aaggcatttc gcacatcgat gccatcagac atgccgcgca ggaggccgtc aaaggcacac
      901 ccctggccgg atccaagatc ttcatcgcgg gcaccgcggc cacctacaaa gacattcagg
      961 acggcgccaa atacgatctg ctgatcgcgg gaatcgccgc gctcagcctg attttgctcg
     1021 tcatgatgtt catcacccga agcatcgtcg ccgcggtcgt catcgtgggc accgtggccc
     1081 tgtcgttggg tgcctctttc gggctttccg tgctgatctg ggaggatctt ctcggtatcc
     1141 cgctgtactg gatcgtgctg gcgctggctg tgatcctgct cttggcggtg gggtcggact
     1201 acaacctgct gctgatctct cggttcaagg aggagatcgg cgccggcttg aacacgggca
     1261 tcatccgggc gatggcgggt tcaggcgcgg tggtgaccgc cgccggtctg gtgttcgcgg
     1321 cgaccatggc ctccttcgtt ttcgccgact tgcggatcct cggtcagatc ggaacgacga
     1381 tcgccctcgg tctgctgttc gacacgctga tcgtgcgttc attcatgacg ccgtcggtcg
     1441 cggcactgct cgggcgctgg ttctggtggc cgctacgggt gcgcacccgc ccccccggcc
     1501 gaaggcttca gccatccgaa tcaggccgat caggtcgtca actacggctg tgggacgacg
     1561 acgattcagc ggtcgccgac gcaccgccac cttcacaagc atgacgatcg agattcggat
     1621 gctcctgcgc gacagttact ctcgctgatc gcgtcgggtc aggtctaccg gccgtggtat
     1681 gcgggctcgg gcgccatcgc cggaacgtca cggagtttcc ccttcatccc cgcgaccctg
     1741 cgcagttgag cggccatgtt catggaggtc aacatcggga taccgccgat gaacgttcga
     1801 aaggagggat tgcctccatc tctgtgcagc atgtagagac agccgaccac atagaagaat
     1861 ccgatggtga agagggcggc gggtatcgcg tacgcgagcg gtgatcgagc atcagcgatc
     1921 agttcgcccg catagtccac ttcgtcctgc gacatcggct cgcgcttgcg gagtttggcc
     1981 agcacagtct tgtgagcgcc gactttctcc cctaatggat gagatgtccg ccgctcaagc
     2041 cgggtcatgt agacgaatac gcacgaggcg aacacgattt ccatcacgac agcaaatatc
     2101 gtgaggtcgc cccacacacc caaatccatt ctctcgcctc tttgtccgtc gaacatcgca
     2161 tcgcgcgggg acgcgagagc cctcgcgacc ctctgaaagg ctatctcaac acgcggttct
     2221 tccccactca cgcctctgcg ccaccgtgtc aggaatggtg ttcgatgtag gcgtcggcgt
     2281 ccttgtcccg attgtcgata cccttactgg tctcgtgttc ggcatagaac cgcaaccatt
     2341 gcatggccag ttcgtgacgc agttcgagcg aggcgctctt gatgaagtcc ggcatggctt
     2401 cacgctcctc ctcgtcgagg tgctcgccat tctccttgcg ggccgcaaac acggcctcga
     2461 accacttctt cgtgcccgcc tcgtgctgac gcgactcgcg caccgcatct cgaatcgcat
     2521 tgtgatc
//
LOCUS       DASYLX010000438         4788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1226114,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000438 DASYLX010000000
VERSION     DASYLX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226114"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGP27_10750"
     CDS             complement(<1..738)
                     /locus_tag="VGP27_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HEV7851817.1"
                     /translation="MSHPLDPLSADEFTAVAVILRREHRVGAGEPATSDTGAGEPATS
                     DTGAGVPATSELGWRIASIELVEPTKTELADFDGGGPSPPRRAEVICLQRSTNATYKG
                     VVTLDDDTVESFDHIPGVQANFTVDEFLECDELVRKHPEFIAALEKRGIIDTDLVFVD
                     TWTYGDAVAPPEYRDRRLGWSDTWRKDGPGQSPYAHPVSGLHCVIDVNTMQVLRVEDN
                     GGVDEPNVMGEYVPKHIPERIRSASRRE"
     gene            complement(893..2401)
                     /locus_tag="VGP27_10755"
     CDS             complement(893..2401)
                     /locus_tag="VGP27_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV7851818.1"
                     /translation="MHEHVVRATTGSGTVEGFSRDGVHRWRSIPFAQPPTGPLRFKAP
                     RPVQPWPGVRYCHGFGHCAPQQRMYTALGVGRYQSMGEDCLTLNVTTPKRRRDEPMPV
                     MVFIHGGGYILGSSATPIYDGAALARKGCVYVSVNYRVGALGCLDLSSLSTADVTIDD
                     NLFLRDLVMALRWVRDNVAAFGGDPDNVTIFGESAGAQAVATLLAVPAAKGLFAHAIS
                     ESPASGMVGTSDVRAQYAEQFASLLGVTKQDAAQALMAARPAKLVNTLDELIKDAQSG
                     MLGAFAVGPTSGTDYLPLDPVEAMRTGTAHPVPLIIGSNAHEGRLFTRWLKLLPTNES
                     MIEALLGGLEPEQRERITSAYPGYPDEAACIRLGGDFAFGTAAWQIAEAHSIHAPTYR
                     YRYDYAPRTLHWSGLGATHATELFAVFDVYRTRFGSLLTAAADGRSARRVSDDVQTRW
                     REFSRTGVPGDDWPAYTHADRAVMVFDRRSHVVHDPHGNRREAWAGFTLASR"
     gene            2477..3166
                     /locus_tag="VGP27_10760"
     CDS             2477..3166
                     /locus_tag="VGP27_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851819.1"
                     /translation="MRIAVLIAASVLVGGCSQGVGGDANRTESSVSAPTRTTTSPPRT
                     TTSTPTPPVKPPEPGADVADAIMFIESGTPADVSRFHTATRDGAATQLGDDVAFTTPS
                     GKTQCMTDSKNFGGALACLVDLTIPPLQPPDVYGHWKGGWVDFAGPSIEIGSAHGDPG
                     RFGNGTGPELPYGSALAFGDYRCRSDPAGLYCANYANQSAARFSDAGIVAFGCLQKVT
                     PPAGIGEKYSC"
     gene            complement(3174..4265)
                     /locus_tag="VGP27_10765"
     CDS             complement(3174..4265)
                     /locus_tag="VGP27_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7851820.1"
                     /translation="MPAKFFWFLPTSGDSRSIVGASHASSNHIVPDTYRKPSRRYLAE
                     VARAADRLGYEGVLTPTGTWCEDAWLTAAALLSETERLKFLVAFRPGLVPPTLAAQQT
                     ATLQRFSEGRVLLNIVTGGDDLEQRRFGDWLSHDERYARTGEFLHIVNSVWHEGSVNF
                     NGDYYTVEDARVSAPPNPLPQIYFGGSSAAALPIAAQYVDVYLTWGEPPEAAAAKIEK
                     VRELAAARGRSVRFGIRLHTISRDTSAAAWAVADELLAELSPEQIAKQTQLHAKSQSE
                     GQRRMTALHGGRIDQLEIYPNLWAGVGLVRGGAGTALVGSHEEVASLIYEYHSVGFDE
                     FILSGYPHLEEAYWFAEGVLPLLRQKGVA"
     gene            complement(4273..>4788)
                     /locus_tag="VGP27_10770"
     CDS             complement(4273..>4788)
                     /locus_tag="VGP27_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7851821.1"
                     /translation="SPSDVGLVFLQPADALGAFTQRQVDAWAIWDPYTAQAEADIPVR
                     SIAEATDVTNGAGFGIASDDALADPKRNTALNDLLVRYAKAVRWAKDNPDQWARDYAA
                     AVGLDPKVSATSQARSLRFPAALSDQLVGSEQQLADLFAESKQIADAPKFANWVDRRY
                     SDALTPLLINR"
BASE COUNT          745 a         1619 c         1623 g          801 t
ORIGIN      
        1 ctcacgtcgc gatgccgagc ggatgcgctc cgggatgtgc ttgggcacgt actcgcccat
       61 cacgttcggt tcgtcgaccc cgccgttgtc ctcgacccgc agcacctgca tggtgttgac
      121 gtcgatgacg cagtgcagcc cgctgaccgg gtgggcgtag ggactctgcc ccggaccgtc
      181 cttgcgccag gtgtccgacc agccgaggcg ccgatcgcgg tactccggag gtgcgacggc
      241 gtcgccgtag gtccaggtgt cgacgaacac caggtccgtg tcaatgatgc cgcgcttctc
      301 cagtgccgcg atgaactcgg gatgcttgcg gaccagctcg tcgcactcga ggaactcgtc
      361 gacggtgaag ttggcctgca cgccggggat gtggtcgaac gattcgacgg tgtcgtcgtc
      421 gagggtcacc acgcccttgt aggtggcgtt ggtgctgcgc tgcaggcaga tcacctcggc
      481 gcggcgaggc gggctcgggc caccgccgtc gaagtccgcg agctctgtct tcgtcggttc
      541 gacgagttcg atggaagcga tccgccagcc caactcggat gtcgccggca ccccggctcc
      601 ggtgtccgag gtcgccggct ccccggctcc ggtgtccgat gtcgccggct ccccggctcc
      661 cacccggtgc tcgcgcctca ggatcaccgc caccgccgtg aactcgtcgg ctgacagcgg
      721 atccaatggg tggctcactc gatcacgcaa tcacatgagg cgatcccgcg caggaggttc
      781 ggctcccttt gagtcccgcc gatcaacgtt tcgcagagct cttctcgcag tttttctgcg
      841 gaacgtcgat cgctgtggct tccgccgcgg cggcggcacg cagcccggct tactagcgcg
      901 acgccaacgt gaagcccgcc cacgcctcgc gacggttgcc gtgcgggtcg tgcacgacgt
      961 gagaccggcg atcgaacacc atcaccgcac ggtcggcgtg cgtgtaggcc ggccagtcgt
     1021 caccgggcac gccggtgcgg ctgaactcac gccatcgtgt ctgcacgtcg tcgctgaccc
     1081 gccgcgccga ccgaccgtcg gcggccgcgg tgagcaacga gccgaaccgc gtgcggtaca
     1141 cgtcgaagac ggcgaacagc tccgtcgcgt gcgtcgcgcc cagccccgac cagtgcaacg
     1201 tgcgcggtgc gtagtcgtag cggtaccgat aggtcggcgc gtgaatactg tgcgcctcgg
     1261 cgatctgcca cgccgccgtg ccgaaggcga agtcaccgcc cagccgaatg caggcggcct
     1321 cgtccgggta gccggggtag gccgaggtaa tacgttcgcg ctgttcgggt tcgaggccgc
     1381 cgagcaaagc ctcgatcatg gactcgttcg tcggcagcag cttgagccat cgggtgaaca
     1441 gcctgccctc gtgggcattg ctcccgatga tgagcggcac ggggtgcgcc gtcccggtcc
     1501 gcatcgcctc gaccgggtcg agcggcaggt agtcggtgcc gctggtcggg ccgaccgcga
     1561 acgcgcccag catgcccgac tgcgcatcct tgatgagctc gtcgagggtg ttcaccagct
     1621 tggcgggcct ggccgccatc aacgcctgtg ccgcgtcctg tttggtcacc ccgagcagcg
     1681 aggcgaactg ctcggcgtat tgggccctga catcggaggt gcccaccatg ccgctggccg
     1741 ggctctccga aatggcatgc gcgaaaaggc ctttggccgc cggcaccgcg agcaatgtcg
     1801 ctaccgcctg cgcgcccgcg ctttcgccga agatcgtcac gttgtccggg tcgccgccga
     1861 acgccgcgac attgtcgcgc acccatcgca gggccatcac gaggtcgcgc aggaacaggt
     1921 tgtcgtcgat ggtcacgtcc gcggtcgaca gcgacgagag gtcgaggcag ccgagcgccc
     1981 ccacccggta gttcacagag acgtagacgc aacccttgcg ggccagcgcc gcaccgtcgt
     2041 agatcggcgt tgcggagctg ccgaggatat agccgccgcc gtggatgaac accatgacgg
     2101 gcatcggttc gtcacgtcgc cgcttgggcg tggtgacgtt cagcgtcagg cagtcctccc
     2161 ccatcgactg atacctgccg acccccagcg ccgtgtacat ccgctgctgt ggagcgcagt
     2221 ggccgaaccc gtggcagtag cgcacacccg gccacggctg caccggccgc ggcgccttga
     2281 atcgcagcgg acccgtcggc ggctgggcga acggaatcga acgccaccgg tgcacgccgt
     2341 cgcggctgaa gccctcgacg gtcccggagc cggtcgttgc gcggacgacg tgttcgtgca
     2401 tgaatcgacc gtatcgaacg aactcgcggg ccgcgccttg ccggtattcg gttgccggcc
     2461 cgctagcctg gccggtatgc ggattgctgt gctgatcgcg gcgtctgtgc tggtcggggg
     2521 gtgttcgcag ggggttggcg gagatgccaa tcggaccgag tcctccgtgt cggccccgac
     2581 aaggacgaca acgtcaccgc cgcgcaccac cacctcgacg ccgacgcccc cggtcaagcc
     2641 gccggaaccg ggtgccgacg tcgccgacgc gatcatgttc atcgaatcgg ggaccccggc
     2701 cgacgtgagc cggttccaca ccgccaccag ggacggtgcc gcgacgcaac tcggtgacga
     2761 cgtcgcgttc accacaccgt cgggcaaaac gcagtgcatg accgactcga agaacttcgg
     2821 cggcgcgctg gcgtgcctgg tggacctcac gattccaccg cttcagccgc ccgatgtgta
     2881 cggccattgg aagggcggct gggtcgactt cgccggcccc agcatcgaaa taggttcggc
     2941 gcacggcgat ccgggccggt tcggcaacgg caccggcccg gaactgccct acggatcggc
     3001 gctcgccttc ggcgactatc ggtgccggtc agacccggcc gggctgtact gcgcgaacta
     3061 cgccaaccag tcggccgccc ggttcagcga cgcgggcatc gtcgccttcg gatgcctgca
     3121 gaaggtgacg ccgcccgcag ggatcggcga gaagtacagt tgctgaaccc gtttcaggcg
     3181 acgcccttct gacgaagcag tggcagcacg ccctcggcga accagtacgc ctcttccagg
     3241 tgcgggtagc ccgacaggat gaactcgtcg aatccgaccg agtggtattc gtagatcagg
     3301 ctggcgactt cctcatggct gccgacaagg gcggtgcccg ccccgccgcg gaccaacccg
     3361 acaccggccc acaggttcgg gtatatctcc agctggtcaa tccgcccacc gtgtaacgcc
     3421 gtcatccggc gttggccctc ggattgcgat ttggcgtgca gctgagtctg tttggcgatc
     3481 tgttcgggcg atagctcagc gagcagttcg tcggcgaccg cccacgccgc tgcggacgtg
     3541 tcacggctga tcgtgtgcag ccgaatcccg aagcgcacgc tgcggcccct tgccgccgcg
     3601 agttcgcgga ccttctcgat cttggcggcg gccgcctccg gcggctcacc ccaggtgagg
     3661 tagacatcga cgtattgagc agcgatggga agggcagcgg ctgacgatcc gccgaagtag
     3721 atctgcggca acgggttcgg cggtgccgac acccgggcgt cctcgacggt gtagtaatcg
     3781 ccgttgaagt tcaccgagcc ctcgtgccag accgagttaa cgatgtggag gaactcccct
     3841 gtgcgggcat agcgctcatc gtggctgagc cagtcgccga agcggcgctg ctcgaggtcg
     3901 tctccgccgg tgacgatgtt cagcagaact cggccctcgg agaaccgctg gagggtggct
     3961 gtctgttgcg cggcaagcgt cggcggcacc aggcccggcc ggaaggcgac caggaacttc
     4021 agccgctcgg tctccgacag cagtgcggcc gccgtcaacc aggcgtcctc gcaccacgtg
     4081 ccggtcggtg tcagcacgcc ctcgtagccg agtcggtcgg ccgcgcgtgc gacctccgcc
     4141 aaatagcggc ggcttggctt tcgataggtg tcgggcacga tgtggttcga cgacgcgtgc
     4201 gaggcaccga cgatcgaacg gctgtcgccg ctggtgggca ggaaccagaa gaatttcgcc
     4261 ggcatgtcgc tcctagcggt tgatcagaag aggagtaagc gcgtcgctat agcggcggtc
     4321 cacccagttg gcgaacttcg gcgcatcagc gatctgcttc gattcggcga agaggtcggc
     4381 caattgctgc tcggagccca ccaattggtc ggacagcgct gccgggaatc gcaggctgcg
     4441 cgcttgcgac gttgcggaga ccttcggatc gagacctacc gccgcggcgt agtccctggc
     4501 ccattggtcg ggattgtcct tggcccagcg caccgccttg gcgtaccgca ccagcagatc
     4561 gttaagcgca gtgttgcgtt tcgggtcggc cagcgcgtcg tcggacgcga tgccgaagcc
     4621 ggcaccgttg gtgacgtctg ttgcctccgc gatgctgcgc accgggatgt cggcctcggc
     4681 ctgcgccgtg tagggatccc agatcgccca tgcgtcgacc tggcgctggg tgaatgcgcc
     4741 gagggcgtcg gcgggttgca ggaacaccag gccgacgtcc gagggcga
//
LOCUS       DASYLX010000439         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1995201,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000439 DASYLX010000000
VERSION     DASYLX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995201"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..421
                     /locus_tag="VGP27_10775"
     CDS             14..421
                     /locus_tag="VGP27_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7851822.1"
                     /translation="MSAPLPEPTPVSQPFWDALREHRILIQYSPSTDRYVFYPRTLAP
                     GTLADDLQWREIDGAGSLYTYTVARRPTGPPWAEKLPQLLAVVEWDVGPRVSTELVDV
                     EPGDIRIGMRVTPVFCDVPDAGITLLRYRPNHV"
     gene            414..1988
                     /gene="fadD1"
                     /locus_tag="VGP27_10780"
     CDS             414..1988
                     /gene="fadD1"
                     /locus_tag="VGP27_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD1"
                     /protein_id="HEV7851823.1"
                     /translation="MFETVQQLLRSRMGDDSVAMVHEDRTWTWQQHLAEAAAEASALI
                     ELADPSRPMHVGALLGNTPAMLRAMAAGALGGYVLCGINTTRRGDGLAADIRRSDCQL
                     VLADSDHMPLLEGLDLGRATVLDVDGSTYRDAVTKAKPLEPYREAEGMDPVVMLFTSG
                     TSGDPKAVRFAHAMAVLCGASLAERFELTVDDVCYLSMPLFHSNGVAAGWAVALACGA
                     TMVPAKFSPSRFLADIRRYGATYMNYVGKPLALVLGTSEKPDDADNTLRAAFGNEATE
                     RDVDEFARRFGCLVVDSFGSSEFAVVVMREDGCPSGSIGRGYPGVSVYHADTVTECAP
                     AVFDGHGALANFDEAVGELVNTYGVGGFTGYYNDPAATDERMRHGMYWSGDLAYRDAD
                     GWIYLAGRTADWMRVDGENLAAGPIERILQRLPEINHVAVYAVPDERIGDQVMAAVVL
                     NEGDTLSPRRFEHFLAAQPDLSPKAWPSYVRINEALPKTATNKILKRTLIQQGVSAAD
                     GLLWRRAARGREYSAQ"
     gene            1985..>2867
                     /locus_tag="VGP27_10785"
     CDS             1985..>2867
                     /locus_tag="VGP27_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV7851824.1"
                     /translation="MTDPLPPEGFYPLPSALPVEVVPARAFVAQPNDDPFYTYSGSTP
                     LDRLPPGTVLATRSVPYHILGLPTLLKTTQLLYRSTSQTGRPTANVTTVIQPLHQSDT
                     SRILSYQSAYDSLNRNDQPSYAINGGLTLGGFVPTVELAVFSLFIAQGFTVIVPDTEG
                     QRADFAAGPEYGMNTLDSIRAAFNASAVETASHANVALLGYSGGAIAGEWAAELAPTY
                     APDVNARLIGAAIGGVLVHPAHNLRYVENTYIWAGVMPMALIGIARAFEVDFTPYLTA
                     RGLELYRAMQSSSIVHVL"
BASE COUNT          461 a          946 c          982 g          478 t
ORIGIN      
        1 tgggtggcga ccgatgagcg cgccgttgcc ggagccgacg ccggtctcgc agccgttctg
       61 ggatgcgttg cgggagcacc ggattctgat ccagtactcg ccgtcgaccg accggtatgt
      121 gttctacccg cgcacgctgg caccgggcac gctggccgac gatctgcagt ggcgcgagat
      181 cgacggcgcc ggctcgctct acacctatac cgtggcgcgc aggccgaccg gaccgccgtg
      241 ggcggagaag ctgccgcagt tgctggccgt cgtcgagtgg gacgtcggcc cgcgggtgag
      301 caccgaactc gtcgacgtcg aacccgggga tattcgcatc gggatgcggg tgacgcccgt
      361 cttctgcgat gtgcccgatg cgggaatcac gctgctgcgc tatcgaccta accatgtttg
      421 agacagtgca gcagctgctg cgatcgcgga tgggcgacga cagtgtcgcg atggtgcacg
      481 aggaccggac ttggacgtgg cagcagcacc tggcggaggc ggcggcggag gcgtcggccc
      541 tgattgagct cgccgacccg tcgcgtccga tgcatgtcgg tgcgctgctc ggcaacacgc
      601 ccgcgatgct gcgggcgatg gcggcgggcg cgctgggtgg gtacgtgttg tgcggcatca
      661 acacgacgcg acggggcgac gggttggcgg ccgacatcag gcggtcggac tgccaactgg
      721 tgctcgccga cagcgatcac atgcccctgc tcgagggtct cgacctgggc cgtgcgacgg
      781 tgctcgatgt cgacggctcc acctatcgcg acgcggtgac gaaggcgaaa ccgctagagc
      841 cctatcgcga agccgagggc atggaccccg tcgtcatgct gttcacatcc ggcacaagcg
      901 gtgatccgaa ggcagtgaga tttgcgcacg cgatggcggt gctgtgcggc gccagcctgg
      961 ccgagcggtt cgagctgacc gtcgacgacg tctgctacct gtcgatgccg ctgttccact
     1021 cgaacggggt ggcggcgggt tgggcggtcg ccttggcgtg cggcgcgacg atggtgcccg
     1081 cgaagttctc gccgtcgcgg ttcctggccg atatccgccg ctacggtgcg acgtacatga
     1141 actacgtcgg caagccgctg gccctcgtgc tgggcacgtc ggagaagccc gacgatgccg
     1201 acaacacgct gcgggcggcg ttcggcaacg aggccaccga acgcgacgtc gacgagttcg
     1261 cccggcgatt cggctgcctc gtcgtcgaca gcttcggttc cagcgagttc gccgtcgtcg
     1321 tcatgcgcga ggacggctgc ccgtcgggct cgatcggcag gggatacccg ggtgtcagcg
     1381 tgtaccacgc cgacaccgtg acggaatgcg cgccggcggt tttcgacggg cacggcgcac
     1441 tggccaactt cgacgaggcg gtcggcgagc tggtcaacac gtatggcgtg ggcggcttca
     1501 ccggttatta caacgacccc gctgccaccg atgagcggat gcggcacggc atgtactggt
     1561 cgggcgacct ggcttaccgc gacgccgacg ggtggatcta tctggccggc cgcaccgccg
     1621 actggatgcg ggtcgacggt gagaacctgg cagccggccc catcgagcgg atcctgcaac
     1681 ggctacccga gatcaaccac gtcgccgtct acgcggtacc cgacgagcgg atcggcgacc
     1741 aggtgatggc ggccgtcgtc ctcaacgagg gagacacgtt gtcgcccaga cggttcgagc
     1801 acttcctggc agcacagccc gacctgtcgc cgaaggcgtg gcccagctac gtgcgcatca
     1861 acgaagcgct gccgaagacg gcgacgaaca agatcctcaa gcgcacgctc attcagcagg
     1921 gcgtcagcgc agcagacggt ttgctgtgga gacgtgcggc tcggggacgg gaatactcgg
     1981 cgcaatgacc gacccactgc caccggaggg cttctatcct ctcccgagtg cgttgcccgt
     2041 cgaagtcgtc ccggctcggg cgttcgtggc gcagccgaac gacgacccct tctacaccta
     2101 ctccggcagt accccgctcg accggctacc gccgggcacg gtgctcgcca cccgcagcgt
     2161 tccgtaccac attctgggcc tgccgacgct cctgaagacc acgcagctgc tgtatcggtc
     2221 gaccagccaa accggtaggc cgaccgccaa cgtcaccacc gtcatccagc cgctgcacca
     2281 gtccgatacg agccggatac tgtcgtatca atcggcttac gactcgctga accgcaacga
     2341 ccagccgtcg tatgcgatca acggcgggct gactttgggc ggttttgtgc ccaccgtcga
     2401 gcttgcggtc ttcagtttgt tcatcgcgca gggtttcacg gtgatcgtgc cggacaccga
     2461 aggccagcgt gccgacttcg ccgcgggccc cgagtacggg atgaacaccc tggactcgat
     2521 ccgcgccgcg ttcaacgcgt cggcggtcga gaccgcgagc cacgctaacg tcgcgctgct
     2581 cggctactcc ggtggcgcga tcgccggcga atgggccgca gaactcgcgc cgacgtacgc
     2641 gccggatgtc aacgcccgct tgatcggcgc ggccatcggc ggcgtgcttg tgcacccggc
     2701 gcacaacctg cgttacgtcg agaacaccta catctgggcg ggtgtgatgc cgatggcgct
     2761 gatcgggatc gcgcgtgcgt tcgaggtcga tttcacgccg tacctgacgg cacgcggctt
     2821 ggagctttac cgtgccatgc agagctcgtc gattgtgcac gtcctcg
//
LOCUS       DASYLX010000440         4511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2213805,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000440 DASYLX010000000
VERSION     DASYLX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213805"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGP27_10790"
     CDS             <1..525
                     /locus_tag="VGP27_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEV7851825.1"
                     /translation="AVVGAGGIGFDVSEFLVTDQSPTLNLKEWKAEWGVADPQEARGA
                     LTTPIPAPAAREVYLLQRTKGSQGKRLGKTSGWVHRASLKAKGVTQLSGVNYERIDDD
                     GLHISFGPDHERRQVLAVDNVVICAGQESVRDLEDGLRRNGIEPHIIGGAALAAELDA
                     ERAIKQGTELAARL"
     gene            complement(648..1196)
                     /locus_tag="VGP27_10795"
     CDS             complement(648..1196)
                     /locus_tag="VGP27_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV7851826.1"
                     /translation="MTAATATTQLTGTWAIDPVHSSIAFSVRHLVVSKVRGHFNTFSG
                     DVVVGEDGTPSVTAEIAIDSIDTRNEQRDAHLKSADFFDAEQYPTATFTSTGVRESGD
                     KYVLDGDFTVKGITRPVSLDLEFNGTNPGMGHGEVAGFTASVVLNRKDFGIDIDMPLE
                     TGGAVVGDKVTITLEIEALKQA"
     gene            complement(1267..1728)
                     /locus_tag="VGP27_10800"
     CDS             complement(1267..1728)
                     /locus_tag="VGP27_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEV7851827.1"
                     /translation="MTTLFGLPAHALLNHAIVVLAPLLAALEILCALWPAARRRLVWF
                     NLALAVGVMVLTPLTISAGEWLYDQESQHRPILQLHQERGEWAIYIAVALLIVAVIQA
                     VHHWRESRSSAPKRALVVIAALLAVVVGVSSIVGVTLIGDAGARAVWSKGE"
     gene            complement(1835..4414)
                     /locus_tag="VGP27_10805"
     CDS             complement(1835..4414)
                     /locus_tag="VGP27_10805"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEV7851828.1"
                     /translation="MALNPQPGAPLPNPVAPPKPSASSPNALRRVLRRARDGVALNVD
                     EAAIAMTARGDDLADLCASAARIRDAGLTSSDRRGPTGRLPVSYSRKVFIPVTHLCRD
                     TCHYCTFVTVPGKLRAQGSGMYMEPDEILDIARRGAELGCKEALFTLGDRPEARWDEA
                     RQWLDERGYDSTLDYVRAMAIRVLEETGLLPHLNPGVMTWSELSRLKPVAPSMGMMLE
                     TTSRRLFETKGLAHYGSPDKDPEVRLRTLDDAGRLSIPFTTGLLVGIGETLTERAETL
                     HAIRKSHKAFGHVQEVIVQNFRAKDHTAMALTPDAGLDDFVATIAVSRLVLGPKMRIQ
                     APPNLVSRDECLALIGAGVDDWGGVSPLTPDHVNPERPWPALDELAAVTAEAGYDLVQ
                     RLTAQPPYVQAGAAWIDPRVQGHVAALADPDTGFALDVKPVGRPWQEPDEASESLGRT
                     DLHSAIDSEGRLTTARSDLDSAFGDWESIREKVGELAARAPERIDTDVVAALRSAERD
                     PGGCSDDEYLALATADGPALEAVAALADSLRRDTVGDDVTFVVNRNINFTNICYTGCR
                     FCAFAQRKGDADAYSLSTDEVADRAWEAHVAGATEVCMQGGIDPELPVTGYADLVRAV
                     KKRVPSMHVHAFSPMEIANGVTKSGLSIREWLISLREAGLDTIPGTAAEILDDEVRWV
                     LTKGKLPTSMWIDVVTTAHEVGLRSSSTMMYGHVDTPKHWVGHLRVLRDIQDRTGGFT
                     EFVPLPFVHQSSPLYLAGGARPGPTHRDNRAVHALARIMLHSRISHIQTSWVKLGVER
                     TQVMLNGGANDLGGTLMEETISRMAGSEFGSAKSVEELTAIAEGIGRPARQRTTTYAP
                     LAA"
BASE COUNT          781 a         1458 c         1498 g          774 t
ORIGIN      
        1 gccgtcgtcg gcgcgggcgg tatcggattc gacgtcagcg agttcctggt gaccgaccag
       61 tctccgacgc tgaacctgaa ggagtggaag gcggagtggg gcgtggcgga cccgcaggaa
      121 gcccgcgggg cgttgacgac tccgatcccc gcgcccgccg cccgtgaggt ctatctgttg
      181 cagcgcacga agggatcaca aggcaagcgg ctcgggaaga cgtcgggctg ggtgcaccgg
      241 gcgtcgttga aggcgaaggg tgtcacgcag ttgtcgggcg tgaactacga acgcatcgac
      301 gacgacggac tgcacatcag tttcggcccc gaccacgagc gccgacaggt gctggcggtc
      361 gacaatgtgg tgatatgcgc aggccaggaa tcggtgcgtg acctcgaaga tggcctgcgc
      421 cgcaacggta tcgagcccca catcatcggc ggggcggcgt tggcggccga gctcgatgcc
      481 gagcgtgcga tcaaacaagg caccgagctc gccgcccgcc tgtagtctcc ccttgcaccg
      541 cgagcggggg gtacctcccc ctgcaccgcg agcgtgcgtg tctgcacggc aacacgccgc
      601 gttctagcga cagtcagcgc acgctcgcgg ccatcgaacg cgcagaatca ggcttgcttg
      661 agcgcttcga tctccaacgt gatcgtcacc ttgtcgccga cgactgcacc gcccgtctcg
      721 agaggcatgt cgatgtcgat gccgaagtcc ttgcggttca gcacgaccga cgccgtgaac
      781 ccggcgacct cgccatgtcc catgcccggg ttggtgccgt tgaactcgag gtcgaggctg
      841 accggcctgg tgatgccttt gacggtgaaa tcgccgtcga ggacatactt gtcgccgctc
      901 tcacgtacac cggtcgaggt gaaggtggcg gtcggatact gctcggcgtc gaagaagtcg
      961 gcggacttca ggtgcgcgtc gcgctgttcg ttgcgggtgt cgatggaatc gatcgcaatc
     1021 tccgcagtca ccgagggggt gccgtcctcg ccgacaacga cgtcgccgct gaatgtgttg
     1081 aagtggcccc gtaccttgct caccaccagg tggcgcacag agaacgcaat cgatgaatga
     1141 accgggtcga tggcccatgt gcccgtgagc tgtgtagtcg cggtggcagc ggtcatgtga
     1201 ctctcctcga ggtttggcat cggcctctcc gacacctcaa agaggtaagc ggacctcagt
     1261 ccggttctat tcccccttac tccacactgc ccgcgcgccg gcatcaccga tgagcgtgac
     1321 gccgacgatc gacgagacac cgacgaccac agcgagaagg gctgcgatta ctaccagtgc
     1381 ccgcttgggc gcggacgacc gtgactcccg ccaatggtgg accgcctgga tgacggcgac
     1441 aatcagcagg gcgacagcga tgtagatggc ccactcaccg cgctcctggt gcaattgcag
     1501 gatcgggcgg tgctgggact cctgatcgta gagccattca cccgctgaga tggtcagcgg
     1561 cgtgagcacc atgaccccta ccgcaagcgc gaggttgaac cacaccagtc ggcgtcgcgc
     1621 ggccggccag agggcgcaga gtatttcgag agccgcgagc agcggtgcga gcaccacgat
     1681 cgcgtggttc agcagagcat gggcaggcaa tccaaacagg gtggtcacag cgctctccta
     1741 ggctggggac ggtggcaggc acttgtctat acgtacggag cctgccattg ggttcaccgc
     1801 tgcgctcggc ttccgtcccg attttcagca gccgttaggc tgcgagcggc gcgtaggtag
     1861 tggtgcgctg ccgtgcgggc ctgccgatac cttcggcgat cgccgtcagc tcctcaaccg
     1921 atttggcgga gccgaactcc gagccggcca tccgcgagat ggtctcctcc atcaaagtgc
     1981 cgccgagatc gttcgcaccg ccgttgagca tcacctgcgt gcgctcgacg ccgagcttga
     2041 cccagctggt ctggatgtga gagatcctgc tgtgcagcat aattcgagcc aaggcgtgga
     2101 cggcacggtt gtctcggtgc gtcggccccg gccgcgcccc gcccgcaaga tacagcggtg
     2161 acgactggtg cacgaacggc aacggtacga attcggtgaa gccgccggtg cggtcctgga
     2221 tgtcgcgtag cacccgcagg tgtccgaccc agtgcttcgg ggtgtcgaca tggccgtaca
     2281 tcatcgtcga actcgaccgc aggcccactt catgcgcggt ggtgacgaca tcgatccaca
     2341 tcgacgtggg tagcttgccc ttcgtcagca cccagcgcac ctcgtcgtcg agaatctcgg
     2401 ccgcggtacc ggggatggtg tcgagacctg cctcgcgcaa gctgatcaac cactcgcgga
     2461 tggacaaccc actcttggtg acaccgttgg cgatctccat cggtgaaaat gcgtgcacat
     2521 gcattgacgg cactcgcttc ttcaccgcgc gaaccaggtc ggcgtatccg gtgaccggca
     2581 gttcagggtc gatgccgccc tgcatgcaga cttcggtcgc cccggctaca tgtgcctccc
     2641 aggcccgatc ggcaacctcg tcggtggaca gcgagtaggc gtcggcgtcg cccttgcgtt
     2701 gggcgaacgc gcagaaccgg cagccggtgt agcagatgtt ggtgaagttg atgttgcggt
     2761 tgacgacgaa tgtgacgtcg tcgccgacgg tgtcgcggcg aagggaatca gccagcgccg
     2821 caacggcttc cagcgccggc ccgtctgcgg tggccaacgc cagatactcg tcgtcggaac
     2881 aaccgcccgg gtctcgctcc gctgaacgca gcgctgcgac gacgtcggtg tcgatgcgct
     2941 caggagcgcg ggcggccagc tcccccacct tttcccggat ggattcccag tcgccgaacg
     3001 cgctgtccaa atcgctgcgg gcggtggtca gccggccctc ggagtcgatc gctgaatgta
     3061 gatcggtccg gccgagtgat tcggacgcct cgtcgggctc ttgccacggt cgcccaacag
     3121 gtttcacgtc caacgcaaaa ccggtgtcgg gatcggccaa cgccgcgacg tgaccctgga
     3181 ctcgggggtc gatccacgcc gcgccggcct gaacgtaggg cggctgggcg gtcagccgct
     3241 gcaccaggtc gtatcccgct tcggcggtga cggcggccag ctcatccaac gccggccacg
     3301 gccgttcggg gttgacgtgg tccggcgtca acggcgagac gccaccccag tcgtcgacgc
     3361 ccgcgccgat cagggcgagg cattcgtcgc gcgaaacgag gttgggcggg gcctggatgc
     3421 gcatcttggg gccgagcacc aaccgggaga cggcgatcgt cgcgacgaag tcgtcgagac
     3481 ccgcgtcggg agtcaaggcc atcgcggtgt gatccttggc ccggaagttc tgcacgatca
     3541 cttcctgcac atggccgaac gccttgtggg acttgcggat cgcgtgcaac gtctcggccc
     3601 gttccgtcaa cgtctcgccg atgccgacca gcaggccggt ggtgaatgga atcgacagcc
     3661 gcccggcgtc gtcaagcgtc cgcagccgga cctcgggatc cttgtccggg ctgccgtagt
     3721 gcgccagccc tttcgtctcg aacagccgac gcgaggtggt ttcgagcatc atccccatcg
     3781 acggggcgac aggcttgagc cgtgacagct cagaccacgt catcacaccc ggattcaggt
     3841 gcggcagaag gcccgtttcc tccagcacgc ggatcgccat cgcgcgcaca tagtccagcg
     3901 tcgaatcgta gccgcgctcg tcgagccact gccgggcctc gtcccatcga gcctcgggcc
     3961 gatcaccgag agtgaaaagc gcttccttgc aacccagttc cgcaccacga cgggcaatgt
     4021 cgaggatctc gtcgggctcc atgtacatgc cgctgccttg agcgcgcagc ttgcccggga
     4081 cggtgacgaa ggtgcagtag tggcaggtgt cccggcacag gtgggtaacg ggaatgaaga
     4141 ctttgcgcga atagctgacc ggcaaccggc cggtggggcc gcggcgatct gatgacgtga
     4201 gtccggcatc gcgtatccgt gcggcgctcg cacaaagatc ggccaagtcg tcgccgcggg
     4261 cagtcatggc gatggccgcc tcgtcgacgt tcagcgcaac gccgtcgcgg gcgcgccgca
     4321 acacacgccg caacgcgttg gggctggacg cgctcggttt tggcggggcg accgggttgg
     4381 gcagaggggc gccgggctgg gggttcagag ccactcccgt gtaaccccac gtatgacgac
     4441 aatcatccgc acgtctcaca ttctgaaaca tcggcccctc gcatatcggc cacagtagtc
     4501 cgcgtatcgg t
//
LOCUS       DASYLX010000441         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1995255,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000441 DASYLX010000000
VERSION     DASYLX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995255"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGP27_10810"
     CDS             complement(<1..666)
                     /locus_tag="VGP27_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV7851829.1"
                     /translation="MTDGTRLALAYLSRVAEPPCPQLTELVSRVGPVEAARCVKTGAV
                     SQGLARSTEARRGIDCAAEDLDLLSRRGGRLITVDDDEWPYLAFSSFAGVDHRERPQA
                     HPPLALWALGEGRLDDMACRAAAIVGTRACTAYGEHVAADLAAGLVERDVTVVSGGAY
                     GIDGAAHRATLATDGFTVAVLAGGIDVPYPSGHSGLLHRIGQQGLVVTEYPPGIRPAR
                     HRFL"
     gene            complement(663..2174)
                     /locus_tag="VGP27_10815"
     CDS             complement(663..2174)
                     /locus_tag="VGP27_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEV7851830.1"
                     /translation="MALGRAFSVAVRGLEGEIVEIEADITSGLPGVHLVGLPDTALQE
                     SRDRVRAAITNCGNSWPMARLTLALSPATLPKMGSVYDLALAAAVLSAHGKKEWGRLE
                     KTLLLGELALDGRVRPVKGVLPAVLTAKREGWPAVVVPVDNLREASLVDGIEVLGART
                     LGQLHAWFSGKAQLDERVQTSSTEAEQDTDLADVVGQVHARYAVEVAAAGAHHLTLTG
                     PPGVGKTMLAQRLPGLLPELAPGESLEVTAIHSVAGLLSGNTPLITRPPFVAPHHTSS
                     VAALVGGGSGMARPGAVSRAHRGVLFLDEYAEVGNSALEALRTPLEDGEIRLARRDGV
                     ARYPARFQLVLAANPCPCASPDPLDCICTSAEKRRYLTKLSGPLLDRVDLRVFMHRER
                     AEAFAVEAGESTAAVRERVRAAREAAAQRWQKHGIRTNAEVSGVLLRRRYRLDKDAMD
                     PLKTALDRGLLSIRGVDRTLRVAWTLADLAGRTSPKVDEVKTALSFRQPGGVQ"
BASE COUNT          350 a          776 c          727 g          331 t
ORIGIN      
        1 gaggaaccgg tgccgcgccg gacggatgcc gggcggatac tcggtgacca ccagaccctg
       61 ctgcccgatc cggtgcagca gtccggagtg gccggacggg tacggaacgt cgatgccgcc
      121 tgcgagcacg gcgacggtga agccgtcggt ggcgagcgtc gcgcggtgcg ccgcgccgtc
      181 gatgccgtat gcaccgcccg acaccaccgt tacgtcccgt tcgacgaggc ccgccgccaa
      241 gtcggccgcg acgtgctcgc cgtaggcggt gcacgctctg gtgccgacga tcgcggcagc
      301 acggcaggcc atgtcgtcga ggcggccttc gccgagcgcc cacagagcca gcggcggatg
      361 ggcctgaggc cgttcccggt gatcgacacc cgcgaaggag ctgaaggcaa gatagggcca
      421 ttcgtcgtcg tcgacggtga tcaagcgacc gccacggcgg ctcagtaagt cgagatcttc
      481 tgcagcacaa tcgatgccgc ggcgagcctc ggtcgaacga gcaaggccct gcgaaacggc
      541 gcccgtcttg acacacctgg cggcctcgac gggccctacg cggctcacca actccgtcaa
      601 ttgcggacag ggcggttcgg ccacccgaga caaatacgcc agcgcgagtc gcgtgccatc
      661 tgtcattgca cgccgcctgg ctgccggaag ctgagtgcgg ttttgacctc gtcgactttc
      721 ggcgacgtcc ggcccgccaa gtcggccagc gtccaggcga cccgcagtgt tctgtcgaca
      781 ccgcggatgc tgagcagtcc gcggtcgagc gcagtcttca gcggatccat cgcatccttg
      841 tcgagccgat agcgccgacg cagcaggacg ccgctgacct cagcgttggt ccggatgcca
      901 tgtttctgcc aacgctgcgc cgccgcttcg cgcgccgccc tcactcgttc gcgcaccgcc
      961 gcggtcgact ctcctgcttc gaccgcaaag gcttctgctc tctcacggtg catgaagacg
     1021 cgcagatcga cgcggtcgag cagtgggccc gacaacttgg tcagatatcg gcgcttctcg
     1081 gcagaagtgc agatgcagtc gagagggtcc ggggatgcac aggggcatgg gttggccgcg
     1141 agcaccaact ggaagcgggc ggggtatcga gccacgccgt cacgccgggc gagccgaatc
     1201 tccccgtctt ccaacggcgt tcgcagtgcc tccagcgcgc tgtttcccac ctcggcgtac
     1261 tcgtcgagaa acaggacgcc gcgatgcgcg cgactgacgg cgcccggtcg ggccatgccc
     1321 gagcccccgc cgacgagcgc tgccacgctc gacgtgtggt gcggcgccac gaacggcggc
     1381 cgggtgatca gcggcgtgtt gcccgacagc agcccggcaa ccgaatggat cgcggtgacc
     1441 tccagcgact cgccgggcgc caactccggc agaaggcccg gaagccgctg cgccagcatg
     1501 gttttcccga cacccggcgg cccagtcagt gtcaggtggt gcgcgcccgc ggcggccact
     1561 tcgacggcgt atcgcgcgtg aacctgcccg acgacgtcgg cgagatccgt gtcctgctct
     1621 gcttcggtgc tggacgtctg gaccctctcg tccagctgcg ccttacccga gaaccaggcg
     1681 tggagttgcc ccaacgttcg ggcgcccagc acctcgatcc cgtcgaccag gctggcctcg
     1741 cggaggttgt cgaccgggac caccacggcc ggccagccct ctcgcttggc ggtcagcacc
     1801 gccggcagca cacctttgac gggcctcacc cggccatcga gggccaattc gccgagcagc
     1861 agggtctttt cgagccgccc ccattccttc ttgccgtgtg ccgaaagcac cgccgcggcc
     1921 agggccaagt cgtagacgga gcccatcttc ggaagcgttg ccggcgagag tgccagcgtc
     1981 agccgcgcca tcggccagct gttgccgcag ttggtgatgg cggcgcggac acggtcgcgg
     2041 gattcctgca acgcggtgtc gggcagacct acgaggtgga caccgggcag gccggaggtg
     2101 atgtcggcct cgatctcgac gatttctccc tccaacccac gcacagccac cgaaaacgct
     2161 ctgcccagcg ccatcagccg accg
//
LOCUS       DASYLX010000442         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3859631,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000442 DASYLX010000000
VERSION     DASYLX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859631"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGP27_10820"
     CDS             complement(<1..515)
                     /locus_tag="VGP27_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7851831.1"
                     /translation="MDPVVALRQIAYYKDRAREDSRRVMAYRNAADIVEALGDTERDR
                     HGAANSWQTLPGIGPKTAKVIAQAWAGAEPEALVELRSSAADLGGGDVRAALRGDLHV
                     HSNWSDGSAPIEEMMLAARDLGHEYCALTDHSPRLRIANGLSPERLRKQLEVIDELRE
                     AVAPLRILTGI"
     gene            complement(533..1102)
                     /locus_tag="VGP27_10825"
     CDS             complement(533..1102)
                     /locus_tag="VGP27_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEV7851832.1"
                     /translation="MTDISPACQRTADVLARVTDEQLDQPTPCEKLRLHELIAHIGML
                     AQAFTAAAHKDFGPLTDTAPTDGAEIDGDWRTAYPQRLEELAAAWKDPKAWQGMTRAG
                     GIELPAEVMGSVALAEVVIHGWDVARAVATPYDCDPATTQACLAHLAQFEASGTEGMF
                     AAAVPIPPDAPDLDRVIAMSGRDPAWHLG"
     gene            complement(1133..1813)
                     /locus_tag="VGP27_10830"
     CDS             complement(1133..1813)
                     /locus_tag="VGP27_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV7851833.1"
                     /translation="MRIGIAGAGAIGRSVATELLAYGHKILLIENNVRHYEPQTVPGA
                     DWLLADACELVSLEEAGIQLCDVVIAATGDDKANLTTSLLAKTEFGVSRVVARVNDAR
                     NEWLFSEAWGIDVAVSAPRALVAAIEGAIDIGHLVRLMELRQGQVSLAKLKLPEDDPM
                     VGKRVRDIVLPENTALAIVVRDGGVMLPQPDDVLRTDDEMLFFAGGAVEDQVRALVHG
                     ATKPVRGR"
     gene            1923..>3388
                     /locus_tag="VGP27_10835"
     CDS             1923..>3388
                     /locus_tag="VGP27_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEV7851834.1"
                     /translation="MSLQQLYLVLLAGGLVLLASIVATRAATTVGLPSLLLFLGVGVI
                     VGEDGLGLHFENYLLADHLGTIALAIILIEGGLTTRFSDIRRVLAPAGMLATLGVGVS
                     MVITAAGAHLLLGMDWQIALLLGAIVSSTDAAAVFSVLRVVPLPKRVAGLLEAESGFN
                     DAPAVILVLMFSATPLELSPVHAIGELVYELGVGAAIGLGCGWVGSVALRRIALPASG
                     LYPLANFGLGLVAFAAAGSAHASGFLAAYLAAVVLANSGLPHRSATRSFAEGLGWLAQ
                     IGLFVLLGLLVDPSDLGSEIVPAIVIGLVLLLVARPASVVISLAGFRVPWKEQVFISW
                     AGLRGAVPIVLATFPIVEGVPDAIRLLNIVFILVVVFTLLQGPSLRTLAHRLGLIPKE
                     ATREIQVEAAPLDVLEAELLTMTVHSPSRLHGVTVLELRLPDPSVITLIIRGGHTFVP
                     QPDTRIAAGDELLIVTTPKTRTAAERRLRAVSRRGKLA"
BASE COUNT          486 a         1177 c         1101 g          624 t
ORIGIN      
        1 tcgataccgg tgaggatgcg cagcggcgca acggcttcgc gcagttcgtc gatcacctcg
       61 agctgcttgc gcagccgctc cggcgacagc ccgttggcga tccgcaggcg cggcgagtgg
      121 tcggtcagtg cacagtactc gtggcccaaa tcccttgcgg ccagcatcat ctcctcgatc
      181 ggcgctgacc cgtccgacca gttggagtgc acatgcagat cgccgcgcag cgctgccctg
      241 acatcaccac caccgagatc cgcagcgctc gaacgcaatt caaccagcgc ttccggctct
      301 gcgcccgccc aggcctgcgc gatgaccttc gccgtcttcg gtccgatgcc cggcaacgtc
      361 tgccagctgt tcgcggcgcc gtggcggtca cgctctgtgt cgccgagcgc ctcgacgatg
      421 tcggccgcat tgcggtaggc catcacccgc cgcgagtcct cgcgcgcccg gtccttgtaa
      481 tacgcaatct ggcgcagtgc gacgaccgga tccatgcctc cagtgtgccc ggtcagccga
      541 ggtgccaagc gggatcgcgt ccgctcatcg cgatgactcg atcaaggtct ggtgcgtcgg
      601 gtggaatcgg caccgccgcc gcgaacattc cctcggtgcc ggacgcctcg aactgggcga
      661 ggtgagcaag gcaggcctgt gtggtggccg ggtcgcagtc gtagggcgtg gcgaccgcac
      721 gggcaacgtc ccagccgtgg atgacgacct cggccagcgc gaccgacccc atgacttcgg
      781 cgggcagttc gatgccgccc gcgcgggtca tgccctgcca cgccttcggg tccttccacg
      841 cggcggccag ttcttccagt cgctgcggat aggcggtgcg ccagtcgccg tcgatttcgg
      901 cgccgtcggt gggggcggtg tcggtcaacg ggccgaaatc cttgtgcgcc gccgcggtga
      961 acgcctgcgc gagcatgccg atatgggcga tcaactcgtg gagacgcaac ttctcgcacg
     1021 gtgtgggctg gtccagttgt tcatcggtga cgcgtgccag tacatcggcg gtgcgctgac
     1081 aggcggggct gatatcggtc atgcaggtac tgaccgcagc gacgccacga actcatcggc
     1141 cccgcaccgg cttcgtcgct ccgtgcacga gggcgcggac ctggtcttcg acggcgccgc
     1201 ccgcaaagaa caacatctcg tcgtctgtcc gcagcacgtc atcgggttgc ggcagcatga
     1261 ccccgccgtc gcggaccacg atcgccaacg cggtgttctc cggcagcacg atgtcgcgca
     1321 cccgcttacc gaccatcgga tcatcctcgg gcagcttcag tttcgccaag ctcacctgcc
     1381 cctgtcgcaa ttccatcagt cgcaccagat gaccgatgtc gatggcacct tcgatcgccg
     1441 ccacgagggc acggggcgcg gacaccgcga catcgatgcc ccacgcctcg ctgaacagcc
     1501 actcgttgcg ggcgtcgttg accctggcca ccacccgcga cacgccgaac tccgtcttgg
     1561 ccagcagcga ggtggtcagg ttcgccttgt cgtcacccgt cgccgcgatc accacgtcgc
     1621 acagttgaat cccggcctct tcgagcgaaa ccagctcgca cgcgtcggcc agcagccagt
     1681 cggcgccggg gaccgtctgc ggttcgtagt gacgaacgtt gttctcgatc aacaagatct
     1741 tgtggccgta ggcgagcagt tccgtggcga cggaccggcc gatcgcgccc gcgcccgcga
     1801 tgccgatccg catcgtctga cgcgcgtccg cggtattcga cgactcatcg gaccgccgcc
     1861 tgaatcggaa cacaaggtca tactgaccca tgcgctgccc tactggttaa ctgcggggtg
     1921 aaatgagttt gcagcagctg tatctggttc tgctggccgg cggactcgtg ctgctggcca
     1981 gcatcgtcgc cacccgtgcg gcaaccacgg tcggcctacc cagcctgctg ttgttcctcg
     2041 gcgtcggcgt catcgtcggc gaagacgggc tcgggctgca cttcgagaac tatctgctcg
     2101 ccgaccacct cggcacgatc gcgttggcca tcattctcat cgagggcggt ctcacgacac
     2161 gtttctccga catccggcga gtgttggcac cggcaggcat gctggccact ctcggcgtcg
     2221 gggtcagcat ggtgatcacg gccgcagggg ctcacctgct gctggggatg gactggcaga
     2281 tcgccctgct tctcggtgcg atcgtgtcgt ccaccgacgc cgcagctgtg ttctccgtgc
     2341 tgcgcgtcgt accgctgccc aagcgtgtcg ccggactgct cgaggcggag tccggattca
     2401 acgacgcgcc tgcggtcatc ctcgtgctga tgttctccgc cacgccgctg gaactgtcgc
     2461 cggtgcacgc catcggtgaa ctggtctacg agctgggtgt cggcgccgcg atcggtctgg
     2521 ggtgtgggtg ggtcgggtca gtcgcgttgc ggcgcatcgc gcttccggca tcagggctgt
     2581 atcccctcgc gaacttcggg ctggggttgg tcgcgttcgc cgcggcgggc agcgcgcacg
     2641 cgagcggctt cctcgccgca tatctcgccg cggtcgtgct cgccaactcc gggctcccgc
     2701 accgctcggc cacccgatcg ttcgcggagg gcctcggctg gctggcacag atcggccttt
     2761 tcgtcctgct cggcctgctg gtcgacccca gcgacctcgg cagcgagatc gtgcccgcga
     2821 tcgtcatcgg gctggttctg ctgttggtcg cccgtcccgc gtccgtcgtc atctccctcg
     2881 ccggcttccg cgtgccgtgg aaggagcagg tctttatctc gtgggcgggg ctgcgcggcg
     2941 cggttccgat cgtgctcgcg acgttcccca tcgtcgaggg cgtgcccgac gccattcggc
     3001 tgctcaacat cgtcttcatc ctggtggtgg tgttcacctt gctgcaggga ccgagtttgc
     3061 gcacgctggc tcaccgattg ggactcatcc cgaaggaggc cacccgagag atccaagtcg
     3121 aggccgcgcc gctggacgtt cttgaggcgg aactgctgac gatgacggtg cactcgccgt
     3181 cgcggttaca cggcgtgacc gtgctcgaat tgcggttgcc cgaccccagc gtgatcacgc
     3241 tgatcatccg gggcggtcac acgttcgtcc cgcagcccga cacccggatc gcggcgggcg
     3301 acgagttact catcgtcaca acgccgaaga cgcgcacggc cgccgaacgc aggctgcgtg
     3361 cggtgagtcg gcgcggcaag ctggccta
//
LOCUS       DASYLX010000443         4632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1336139,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000443 DASYLX010000000
VERSION     DASYLX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336139"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1116)
                     /locus_tag="VGP27_10840"
     CDS             complement(130..1116)
                     /locus_tag="VGP27_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEV7851835.1"
                     /translation="MQLTREIAEIGAPLEPLSRETIHHFRVQRAGVGLDGFVDGGTLL
                     EWIDTAAHSTAAQWCAGHCVAASIGHLHLHRPIGVGELVEVRATLVYTGHSSMHILIT
                     VSSSDPARAKALQTAQCAMVFVALGDAGEPVEVPRWTPLTMLDLQQHRQARARIRMRR
                     HIEGAMEAEDFTPEDTATGTTLRFRGGSTDVTSDGKVRGGRVMRWIDEAAYACGTGWI
                     GADVVTSYVTGIRFYRPVFVGHAVDVTARIIHTGPRSGHMSVRVMTVGESPRLIAEGV
                     IVVVSPGAHGEARAVRQWRPVSGKDIRLNGQARHLIELRQHFEPFSAVIAST"
     gene            1338..2696
                     /locus_tag="VGP27_10845"
     CDS             1338..2696
                     /locus_tag="VGP27_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851836.1"
                     /translation="MQTEQATRSVAANVIRGSLGNLIEWYDWYAYAAFSIYFASVFFP
                     SGNQTAQLLNTAGVFAVGFLVRPLGGWMFGRYADRFGRRAALTLSVSLMGVGSLGIAL
                     LPGYAQIGALAPILLVALRLLQGLSLGGEYGTSATYLSEVATPHRRGFYSSFQYVTLT
                     SGQLVALGVQIILQQILTSAQMHSWGWRIAFVIGAAAAVTVMWLRRTMDESANYKLAV
                     AESSADADRGSLRVLLSYPRECLTVVGLTLGGTIAFYTFTTYMQKFMINTTGLPKEQV
                     TWINFVALLLFVCLQPAFGALSDRIGRRPLLIAFGVGATLCTVPLLTAVSHAQSALAA
                     FGLMMLGLLIVSGYTSINAVVKAELFPARIRALGVGLPYALTVAIFGGSTEYVALWLK
                     NIGHENWFFFYVAGCGLISLMVYVFMGETSRRSQLERETRELASGNTEQAKVADVLAD
                     SA"
     gene            2707..3372
                     /locus_tag="VGP27_10850"
     CDS             2707..3372
                     /locus_tag="VGP27_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7851837.1"
                     /translation="MQVLLVEDDEGVASALAELLGHAGASVVRTGRGADALHRVKGCD
                     LVLLDLGLPDMDGLDVLRTLRRASTVPVIIMTARDSDGAVVLGLRAGADDYLVKPVRK
                     AVLLARIEAVMRRLRRVGGEHSGSSGPVVAGPVTIDPERREVAVAGVAQALTKTEYQI
                     VATLAARQGEAVSREELMLEVWGTAMVGRSRSLDFFIGQIRVKLTGLPLHTVRGFGFR
                     LDT"
     gene            3374..>4632
                     /locus_tag="VGP27_10855"
     CDS             3374..>4632
                     /locus_tag="VGP27_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7851838.1"
                     /translation="MGLRVRAVLVVLLLLAVAALAVPLALSLADRRTAELAAERDRQL
                     ASVADAAVRQDTPLQRVIERYYDVYGEGVLVIDSDGHTLASRGLDVSDAGVAQAASHA
                     LVDAPAAQWDSIFPWDRTRLLATSGIRDGGELVGAVVLAVDPTVAARDVAIGWLWAAV
                     GCLVLLALAVVVGRALTRWVLRPLDGLERAVAEMTEGIAGPPAGVAGPPELRHFTSAF
                     NTMAQLVRASLDRQRRLIADASHQLRNPLAAVRLRADTLEDYVLKDGRPTYNAMSAEL
                     DRFENLLEQLLRLARAEQVSGSRQVGLSNPAAESADLADVIEERAAYWTPVLSEKDQQ
                     LINRSPHPGPTVQLARHELEQLLDVALENAVRYAGAGTTVTVATEKSDQGVELVISDD
                     GTGLPDEDLAQASTRFWRAHNDLAGTGL"
BASE COUNT          746 a         1454 c         1575 g          857 t
ORIGIN      
        1 aacaacaccg cgctcaaccc gatgctggtc cagatccagg gcgggcaggt caaggccatc
       61 gcgccgccgg actcggcaca ggcgaagatg atctatccgc ttccgccgct gggctgagcg
      121 cggtcagcgt caggtactgg cgatcacggc tgagaatggt tcgaagtgtt gcctgagttc
      181 gatgaggtgc cgagcttgcc cgtttagccg aatgtctttg ccggaaacgg gtcgccactg
      241 acgaacggct cgtgcttcac cgtgcgcacc gggtgacacg acgacgatta ccccttcagc
      301 gatgagtcgc ggcgattctc caacggtcat tacgcgcacg ctcatatgcc cactccgggg
      361 accggtgtga ataattcgtg cggtgacgtc gacggcgtgg ccgacgaaaa cgggccgata
      421 aaaacggatc ccggtcacgt aggacgtgac gacgtcggcg ccgatccaac cggtcccaca
      481 cgcgtaggcc gcttcatcga tccagcgcat cacccgaccg ccgcgaacct tgccgtcgct
      541 ggtgacgtcg gtagatcctc cgcgaaagcg cagcgtggtg cccgtggcgg tgtcctccgg
      601 ggtgaagtcc tcggcctcca tagcaccttc gatgtgtctg cgcatccgta tccgggcgcg
      661 cgcctggcga tgctgttgca ggtcgagcat ggtcagtggc gtccaccgcg gcacttccac
      721 gggctcaccg gcgtcgccga gggcaacgaa aaccattgca cactgcgcgg tttgaagtgc
      781 cttggcccga gccggatcgc tcgaggagac ggtgataaga atgtgcatgc tgctgtgccc
      841 ggtatagacc agggtggcgc gcacctcgac gagttcaccc acaccgatgg gcctgtgaag
      901 gtgaaggtgg ccgatggatg cagcgacgca gtggccggca caccactgcg cggcggtcga
      961 gtgggcggcc gtgtcgatcc attcgagcag cgtgccaccg tcgacgaagc cgtcgagacc
     1021 gacgcctgcg cgctggacgc ggaagtggtg aattgtttcg cggctgagcg gctccagcgg
     1081 ggcaccgatc tcggcgattt cacgtgtgag ctgcatgtca tcccctcctc ctgcacgctc
     1141 catgatgtgg gctgcgcggc cgacccggaa gagatacctg actgatcgcg gtgtttccta
     1201 gcacgatcca cagactgacc gcgccaatct gtggattgtg caagaaatcg ccgtcccggc
     1261 ggccggatga cttccctgac ctcgatgaag tcaacaagat cagcggtacc tacatgccga
     1321 caaaggggtt gaggattatg cagacagaac aagccaccag atcggtggcc gccaacgtga
     1381 ttcggggttc actcggcaac ttgatcgagt ggtacgactg gtatgcctac gcggcgttca
     1441 gcatctactt cgcgagcgtg ttctttccgt cggggaatca gaccgcgcag ctgttgaaca
     1501 ctgcgggtgt tttcgcggtc ggtttcctgg tgcggccact gggcggctgg atgttcggtc
     1561 gatatgccga ccggttcggg cgccgcgcgg cgttgacgct gtccgtgtcc ttgatgggtg
     1621 tcggctcact tggcatcgcc ttgctgccgg gctatgccca gatcggggct ctggcgccga
     1681 tcctgctggt cgcgctccgg ctgctgcagg gcctttcgct gggaggcgaa tacggcacgt
     1741 cggcaacata tctgagtgaa gtcgccaccc cgcaccgtcg gggcttctac tcgtcgttcc
     1801 aatacgtgac gctgacgagc ggtcagcttg tggcgctggg ggtgcagatc atcctgcagc
     1861 agatcctcac ctcggcgcag atgcacagct ggggatggcg gatcgcgttc gtcatcggtg
     1921 cggccgcagc ggtgaccgtc atgtggttgc ggcgcacgat ggacgagtcg gcgaactaca
     1981 aacttgcggt cgccgagagc agtgcggacg ccgaccgcgg cagcctgcgg gtcctgctct
     2041 cctacccgcg ggagtgcctg accgtggtgg gcctcacgct cgggggcacc atcgcgttct
     2101 acacgttcac cacgtacatg cagaagttca tgatcaacac gaccggcctg ccgaaggaac
     2161 aggtgacgtg gatcaacttc gtcgcgctcc tgctcttcgt atgcctgcag cctgcgttcg
     2221 gcgcgctgtc ggaccggatc ggcaggcggc cactgctcat cgcgttcggt gtcggtgcga
     2281 cactgtgcac ggtgccgctg ctcaccgcgg tgtcacacgc gcagtccgcg ctggcggcgt
     2341 tcgggctgat gatgctcggc ctgctgatcg tgtccggcta cacgtcgatc aatgccgtgg
     2401 tcaaggcgga gctcttcccc gcccgtatcc gggcgctggg cgtcggcctg ccgtatgcgc
     2461 tcaccgtcgc gatcttcggt ggcagcaccg aatacgtcgc gctgtggttg aagaacatcg
     2521 gtcacgagaa ttggttcttc ttctacgtcg cgggttgcgg gttgatctcg ttgatggtct
     2581 acgtcttcat gggcgaaacg tcacgccgtt cgcagttgga gcgtgagacg cgtgagctgg
     2641 cttcgggcaa taccgaacag gctaaagtcg ccgacgttct ggccgactct gcttaacatc
     2701 agcgccatgc aggtactgct ggtcgaggat gacgagggcg tcgcgtcggc gctggccgaa
     2761 ctactcggtc acgccggcgc ctcggtcgtg cggaccggcc gcggcgccga cgccctccat
     2821 cgcgtcaaag gctgcgacct ggtgctgttg gatctgggcc tgcccgatat ggacggcctg
     2881 gatgtgctgc gcacgctgcg gcgggcgtcg acggtgcctg tgatcatcat gaccgcccgc
     2941 gacagcgatg gcgccgtcgt gctggggctg cgcgccgggg ccgacgacta tctggtcaag
     3001 ccggtgcgta aagccgtgct gctggcgcgg attgaagccg tcatgcgacg gctccgccga
     3061 gtcgggggtg agcactccgg ttcgtcggga ccggtagtcg ccggtcccgt caccatcgac
     3121 cccgagcgca gggaggtggc ggtcgccggc gtcgcgcaag cgctgacgaa gaccgagtat
     3181 cagattgtcg ccacgctggc agcccggcag ggtgaggcgg tcagtcgcga ggagctcatg
     3241 ctcgaggtat ggggcaccgc aatggtcggt cgctccagat ccctggattt cttcatcggg
     3301 cagatcaggg tcaagctcac cgggcttccg ctgcacacag tgcgcggctt tggttttcgg
     3361 ctggacacct gacatgggtt tgcgggttcg tgccgtcctc gtcgtgctgt tgcttctggc
     3421 ggttgcggca ctcgcagtgc ccctggcgct gagcctggcg gaccgccgaa ccgcggaact
     3481 tgcggcggaa cgcgaccggc agcttgcgtc ggtggctgat gcggccgtcc gacaggacac
     3541 cccgttacag cgggtgatcg agcgttatta cgacgtgtac ggcgaagggg tgcttgtcat
     3601 cgattccgac gggcacaccc ttgcctcgcg cggtctcgat gtctctgacg caggtgtcgc
     3661 gcaggccgcg agccatgctc tcgtcgatgc accagcggca cagtgggatt cgatctttcc
     3721 gtgggatcgc acgaggctgc tggcgacctc cggtatccgt gacggcggcg agctcgtcgg
     3781 cgccgtcgtg ctcgcggtcg acccgacggt ggcagcgcgc gacgtcgcaa tcggctggct
     3841 ctgggcggcg gtgggctgtc tggtgctttt agcgctggcc gtggtggtcg ggcgggcact
     3901 gaccaggtgg gtgctgcggc ctctcgacgg actggagcgt gcagtcgccg agatgaccga
     3961 agggatcgca gggccacccg ccggtgtggc cggcccacct gaactgcggc atttcacctc
     4021 ggcgttcaac acgatggcac agctggtccg ggcctccctg gaccgtcagc ggcggctgat
     4081 cgccgacgcg tcgcatcaat tgcgcaatcc gctggccgca gtccggctgc gagcggacac
     4141 gctcgaggac tacgtgctca aggacggacg gccgacctac aacgcgatgt ccgcagagct
     4201 ggaccggttc gaaaacctgc tggaacaact gctgcgcctg gcccgcgcgg aacaggtcag
     4261 cgggagccgt caggtcggcc tatccaaccc ggccgcggag tcggcggacc tcgcggatgt
     4321 gatcgaggaa cgcgcggcgt actggacccc ggtcctctcc gaaaaagacc agcagctcat
     4381 caaccgctcg cctcatcccg gcccgacggt gcagctcgcg cgccacgagc tggaacagtt
     4441 gctcgacgtc gcgctggaaa acgcagtgcg ctacgccggt gcgggcacga cagtaacggt
     4501 ggcgaccgag aagagcgacc agggcgtcga actcgtcatc agcgacgacg gtaccggcct
     4561 gcccgacgag gacctggctc aggcatcgac ccggttctgg cgtgcgcaca acgacctcgc
     4621 cggcacgggt ct
//
LOCUS       DASYLX010000444         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_348882,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000444 DASYLX010000000
VERSION     DASYLX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348882"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VGP27_10860"
     CDS             <1..888
                     /locus_tag="VGP27_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV7851839.1"
                     /translation="TGICVVSNPEFLREGHAINDFRHPDRIIVGADDDREADRVAEIC
                     GGGGTYTLRMSPESAELAKYASNAFLAVKISYANSLARLCARVGADVEDVTRCMGADV
                     RIGAHFLQPGPGWGGSCLPKDTASLLHSGRSNGVGLPEVAAARETNAAQSGRIAEVLG
                     GAMGVPLQVSRITALGLTFKAGTNDVRDSPALTICADLARAGAQVTGYDPRLASIDPA
                     PLRQASIVTVDDPYLAAKESDAIVVLTEWQEFRNLDWPVIADQAPGAVVVDTRNLLDP
                     GVLADAGLRHIGNGIPGGF"
     gene            complement(885..1901)
                     /locus_tag="VGP27_10865"
     CDS             complement(885..1901)
                     /locus_tag="VGP27_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV7851840.1"
                     /translation="MRLRRSVVSGPGLSRVRRGRGFSYHDQEGKTLTDEAALSRIKGL
                     VIPPAWQKVWISPYENGHIQAVGTDAAGRKQYLYHETWQEERAEEKFDRVLEMSKTLP
                     AMRRRIAQDLRKRGLDRDRVLALALQLLDLGYFRAGGEQYAEENNSFGISTLLCEHVS
                     LQRKAVEFDFPAKSGVRRTLLIDDPEVVRSVRALLRRPERTERFLVCQDSSGPMDIHA
                     DDLNARFKQLVGAEYTVKDLRTWHGTVLAAAAFVDADPPVNKTVIKRVESAVMKEVSE
                     ELGNTPAVARSSYVDPRVVQGYEKGMTIAAGVRRADRIRNRGDQQAILEESTARLIRK
                     VARS"
     gene            complement(1951..2085)
                     /locus_tag="VGP27_10870"
     CDS             complement(1951..2085)
                     /locus_tag="VGP27_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851841.1"
                     /translation="MNHFQPSHTPRRILVTAGVALALLVVVAAAVYAVAFLVLAPMMQ
                     "
     gene            2201..2629
                     /locus_tag="VGP27_10875"
     CDS             2201..2629
                     /locus_tag="VGP27_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7851842.1"
                     /translation="MADEKNNGHQRGDRSVEVRVSAQLENLAVVRTVVAAIGTFEDLD
                     FDAVSDLRLAVDEACTRLIRSAMPDSTLVVVVHPRDTEVVVEASTTCKTQDILTPGSF
                     SWHVLSSLTDEVKTFQDGQGAEEGHVFGISMTTRRASSLR"
BASE COUNT          447 a          869 c          898 g          485 t
ORIGIN      
        1 accggcatct gcgtggtgtc caatccggag ttcctgcgcg agggccacgc catcaacgat
       61 ttccgtcatc ccgaccgcat catcgtcggt gccgacgatg accgtgaggc cgaccgggtc
      121 gccgagatat gcggcggcgg aggcacatac acgttgcgga tgagccccga aagcgccgaa
      181 ctcgcgaagt acgccagcaa cgcgttcctg gccgtgaaga tttcgtacgc caattcgctt
      241 gcccggctgt gtgcccgtgt cggcgccgat gtcgaggatg tcacccggtg catgggagcc
      301 gatgtccgga tcggagcgca tttcctgcag ccgggccccg gctggggcgg ttcgtgcctg
      361 cccaaggaca ccgcttcgtt gctgcacagc gggcggtcca acggcgtcgg gctgcccgaa
      421 gtggcggccg cccgcgagac gaacgccgcc caatcgggtc gcatcgccga ggtcctcggc
      481 ggcgccatgg gtgttcccct gcaggtgtcg cggatcaccg cactgggcct gacgttcaag
      541 gcaggtacga acgatgtgcg cgactcgccc gcgttgacca tctgcgccga cctggcccgt
      601 gcaggcgcac aggtcaccgg ctacgacccc aggctcgcgt cgatagatcc ggcgccgctg
      661 cgacaggcgt cgatcgtcac ggtcgacgat ccctatctgg ccgccaagga gtccgatgcc
      721 attgtggtgt tgaccgagtg gcaagagttc cgcaacctcg attggcccgt gatcgccgat
      781 caggctcccg gcgcggtggt ggtcgataca cgcaacctgc tcgacccggg cgtgctcgca
      841 gacgctgggc tcagacacat cggcaacggc atcccgggtg ggttctagga gcgcgccacc
      901 ttccggatca gtcgcgcggt gctctcctcc aggatcgcct gctgatcgcc gcgattcctg
      961 atgcggtcgg cgcggcgcac acccgccgcg atggtcatgc ccttctcgta gccctggacc
     1021 acccgagggt cgacatagga gctgcgggcc accgccgggg tgttgccgag ttcttcggag
     1081 acttctttca tcaccgcgga ctccacccgt ttgatcacgg tcttgttgac cggcgggtcg
     1141 gcgtcgacga aggccgcggc cgcgagcacg gtgccgtgcc aggtgcgcag gtccttgacg
     1201 gtgtactcgg cgccgacgag ttgcttgaac cgggcgttga gatcgtcggc gtggatgtcc
     1261 atcgggcccg atgagtcctg gcacaccagg aaacgttcgg tgcgttccgg tctgcgcagc
     1321 agcgcgcgca ccgaacgcac cacctcggga tcgtcgatga gcagcgtgcg gcgcacgccg
     1381 ctcttggcgg gaaagtcgaa ctcgacggcc ttgcgttgca gcgaaacgtg ttcgcacagc
     1441 agcgtggaga tcccgaacga gttgttctcc tcggcgtact gctcgccgcc ggcacggaag
     1501 taaccgaggt cgagcagttg cagagccagg gcgaggaccc ggtcgcggtc gagccctcgc
     1561 ttgcgcaggt cttgggcgat gcgccggcgc atggcgggca gcgttttcga catctccagc
     1621 acccggtcga atttctcctc ggcgcgctcc tcctgccagg tttcgtggta gaggtactgc
     1681 ttgcggcccg ctgcatcggt tccgacggcc tggatgtggc cgttctcata cgggctgatc
     1741 cacaccttct gccacgccgg cgggatcacc agtcccttga tccgcgaaag cgccgcctca
     1801 tcggtgagcg tcttgccttc ttgatcgtga taggagaacc cccggcctcg tcgcacccgg
     1861 ctcaggccgg gcccgctgac cacgctccgg cgcagccgca tcaaaacctg ccgttatgaa
     1921 ttcgctcagg ctgggtatca aacatcgatg tcactgcatc atcggcgcga gcacgaggaa
     1981 cgccacggcg tagaccgctg ccgcgaccac taccaacaga gcgagcgcaa cgcccgctgt
     2041 caccaggatg cgacgcggcg tgtgcgaggg ctggaagtgg ttcatgccga ggtattaccc
     2101 cgccgaacgg cgggtcacac gtcatcaatc gtctggagcg ccggtggtta gctggaatgc
     2161 cacttggagt agtgtcgggt cgacatttcc gggaggtcac atggccgatg agaagaacaa
     2221 cgggcaccaa cggggtgacc gttcggtcga agtgcgggta tccgcgcagc tggaaaacct
     2281 cgccgtcgtg cgcaccgtcg tggcggccat cgggaccttc gaggacctcg atttcgatgc
     2341 ggtgtccgat ttgcggctcg cagtggacga ggcctgcacc cggctgatcc gctcggccat
     2401 gcccgactcg acgctggtgg tcgtggttca tccacgcgac accgaagtcg tggtcgaggc
     2461 gtcgaccacg tgcaagacgc aggacatatt gacccccggc agcttcagct ggcatgtgct
     2521 gagttcgctc accgatgagg tgaagacgtt tcaggacgga cagggcgccg aagagggtca
     2581 cgtattcggc atatcgatga cgacgaggcg agcgagttcg ctcaggtgag tcggcagtcg
     2641 tcgcagggtt cgtcgtcacg gcagaactct gaatatgcgg atgtcgcagg catgttccg
//
LOCUS       DASYLX010000445         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1446256,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000445 DASYLX010000000
VERSION     DASYLX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446256"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VGP27_10880"
     CDS             <1..688
                     /locus_tag="VGP27_10880"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7851843.1"
                     /translation="ARAVQIAVEEGVVELDTAAGPPESTVRHLLAHTSGYSMHTDQVM
                     AAPGKRRIYSNYGFGVAAQTVAKEADIDFGRYLAEAVFEPLGMGSSELRGGAEAAGYG
                     GASTVADLAVFAVDLLKPALVSAQMHAEATAVQFPGLDGVLPGFGVQRPNDWGLGFEI
                     RDAKSPHWTGSANSRSTYGHFGQSGTFLWVDPDRDLALIVLTDREFGDWAYEPWPTLS
                     DAVIAEFGSN"
     gene            811..1323
                     /locus_tag="VGP27_10885"
     CDS             811..1323
                     /locus_tag="VGP27_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEV7851844.1"
                     /translation="MRASNQFAESTTGVVYIHASPAAVCPHVEWALSSTLRSRANLTW
                     TPQPAMPGQLRAVTNWVGPVGAGAQLANALRSWSVLRFEVTEDPSAGVDGQRFCHTPQ
                     LGLWSGAMSANGDVMVGEMRLRSLMSAGADMLAAELDSVLGTAWDDALEPYRSGGDTG
                     EVSWLNRGVG"
     gene            complement(1405..>1762)
                     /locus_tag="VGP27_10890"
     CDS             complement(1405..>1762)
                     /locus_tag="VGP27_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628374.1"
                     /note="involved in the biosynthesis of
                     phosphatidylinositol mannosides (PIMs); the enzyme from
                     Mycobacterium tuberculosis can phosphorylate a variety of
                     amphipathic lipids; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV7851845.1"
                     /translation="RLSFDGEEMETELTLAAFGNTKSYGGGMMICPNADPTDGLLDAT
                     MVASASRTKLIRLFPTVFKGTHVDLEEVRTARARTITVDSPGINAYADGEYVCPLPVE
                     VSAVPGALKILRPANN"
BASE COUNT          287 a          563 c          620 g          292 t
ORIGIN      
        1 cgcgcgcgcc gtgcagatcg cggtcgaaga aggcgtcgtc gagctggaca ccgctgccgg
       61 tccaccggaa tcgacggtgc ggcatctgct ggcgcatacc tccggctact cgatgcacac
      121 cgatcaggtg atggcggcgc cgggaaaacg ccggatctac tccaactacg ggttcggtgt
      181 ggcggcgcag acggtggcca aggaggcgga catcgatttc ggccgctacc tggctgaggc
      241 ggtcttcgag ccgctcggca tggggtccag cgaactccga ggtggcgccg aggccgcggg
      301 ctacggcggc gcatcgacgg tcgccgacct cgccgttttt gctgttgacc tgctcaaacc
      361 cgcgctggtg tcggcccaga tgcacgccga ggccaccgcc gtgcaatttc ccggcctcga
      421 cggggtgctg cccggcttcg gggtgcagcg gcccaacgac tggggccttg ggttcgagat
      481 ccgggacgcc aagtcaccgc attggacggg atcggccaac tcacggagca cgtacgggca
      541 tttcggccag tcagggacgt ttctctgggt cgacccggac cgcgacctgg cgctgatcgt
      601 gctgaccgat cgcgaattcg gcgactgggc gtacgagccc tggccgacgc tgtcggacgc
      661 cgtcatcgcc gaattcggta gcaactagcc cggggactag cgcaacaggg gcctcacaag
      721 gcacaatagg cacgtacgac acaactgcag ctcatggcat tcaccaggtc gttggggaag
      781 acgtccctcg tggagccgaa ggagtagcag atgcgtgcgt caaaccagtt tgccgagtcg
      841 acgacgggcg tggtctatat ccacgcctca ccagcggccg tgtgcccgca tgtcgagtgg
      901 gcgttgtcgt cgaccctcag gtcgagggcg aatctgacct ggaccccgca gccggcgatg
      961 cccggacaat tgcgtgcggt caccaactgg gttggtccgg tcggcgcggg cgcgcagctc
     1021 gccaacgcct tgcggtcgtg gtccgtgctg cgcttcgagg tgaccgagga tcccagcgcc
     1081 ggcgtggacg ggcagcgctt ctgccacact ccacagctcg ggttgtggag cggcgcgatg
     1141 agcgccaacg gcgacgtgat ggtcggcgag atgcggctgc ggtcgctgat gtccgcaggc
     1201 gctgacatgc tcgcggccga actcgactcg gtgctcggca ccgcgtggga cgacgccctc
     1261 gagccttacc gcagcggcgg cgacaccggc gaggtgagct ggctaaaccg cggcgtcggc
     1321 taggttccac ggcggtttgc gcttgccagt atgcgaaatc gcacgcagtg tcggcgagtc
     1381 ccgtacgaaa ccgcacaagc ggtctcaatt attggcgggc cgcagaatct tgagcgcgcc
     1441 cggcaccgcg gacacctcga ccgggagcgg gcagacgtat tcgccgtcgg cgtaggcgtt
     1501 aatgccgggg gagtcgacgg tgatcgtgcg ggcgcgcgcg gtgcgcacct cctcgaggtc
     1561 gacgtgggtc cccttgaaca cggtggggaa caagcgaatc agcttcgtgc gcgacgccga
     1621 ggccaccatg gtcgcgtcga gcagaccgtc ggtgggatcg gcgttcggac agatcatcat
     1681 gcccccgccg tagctcttcg tgttgccgaa tgcggcgagg gtcagctccg tctccatttc
     1741 ctcgccgtcg aacgacagtc gg
//
LOCUS       DASYLX010000446         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1557326,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000446 DASYLX010000000
VERSION     DASYLX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557326"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VGP27_10895"
     CDS             <1..532
                     /locus_tag="VGP27_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7851846.1"
                     /translation="ASDTNGNGDFSRNQFLQGRMALFQSGTYNLAAIAEQSRFRWGVV
                     PMPVGPVGRVSVTNGIAAAGNSATGHPDAVRQVLDWMGSAGGNQYLGAHGAAIPAVLA
                     AQRVYFDYWARRGVDVGPFFRVLDGPRIPAPGGPGFPAGYQAMEPYFDEMFLGRSDVA
                     TTLSDAQAAANAAAER"
     gene            complement(529..1362)
                     /locus_tag="VGP27_10900"
     CDS             complement(529..1362)
                     /locus_tag="VGP27_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV7851847.1"
                     /translation="MTAPARRQQRRPVVLLRPVPGRTVVHDLWAGTKLIVVAAIGVLL
                     TFYPGWVPIGAVAALVLITAWLARVPRGVLPSIPRWLWFLLMLGGVTAALAGGSPVIA
                     LFGQDLGLGGLLKFLRITALSIVLLALGGMVSWTTNVAEIAPAVAKLGRPLRRLRIPV
                     DEWAVTLALALRAFPMLIDEFRTLYAARSLRPKPVLTTRRARRRRWAFEVIDLIAAAV
                     IVALRRADEMGDAITARGGAGQISAAPSGPKLRDWVALSLVLLVCGAALALELTLLGT
                     T"
     gene            complement(1359..3425)
                     /locus_tag="VGP27_10905"
     CDS             complement(1359..3425)
                     /locus_tag="VGP27_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEV7851848.1"
                     /translation="MTATRRASDTRRRGSLQPIELAHSSVMAALCAATAIIAVVVPFA
                     GGVSLLGNVPMGLLVYRFRVRALIAATVAGATIAFLIAGMGGFMTVINCAYIGLLIGL
                     ILRRGRGWPTVVLVGLVAGAVFGGAVVAALSVLVRLRHLIFESMTANVDGIAAVLTSF
                     PQWWWLPDLDHLAESLRHDFATALHYWPVLLGGSSVLSITIVMLIGWWALSQVINRLV
                     VIPDVHKLDSAKDARPVAPVPVRLTDVRFRYPKSDHDALGPVSLTVEPGEHVAVTGPN
                     GSGKTTLMLVLSGREPSSGTVERPGSVGLGRLGGTAVILQHPESQVLGTRVADDVVWG
                     LPSGTVTDVHRLLAEVGLDGLAERDTGGLSGGELQRLAVAAALAREPSLLIADEVTSM
                     VDQDGRDGLMGVLSGLTEHHRMALVHITHYNDEADSADRAIDLTGNRGATDNTEMVET
                     TAAPDATGPLKPRSDAPVLELIDVNHEYGSATPWATEALRNINFAVDEGDGVLIHGLN
                     GSGKSTLAWIMAGLAVPTSGSCLLDGAPVSDQVGAVAISFQAARLQLLRSRVDVEVAS
                     AAGFSPHDRARVIEALATVGLDAALADRRIDQLSGGQMRRVVLAGLLARSPRALILDE
                     PLAGLDAASARGLLRLLEELRRNAGLTVIVISHDFTGLEDLCPRILHLQDGVLAPAST
                     AAGGMS"
     gene            3530..>3748
                     /locus_tag="VGP27_10910"
     CDS             3530..>3748
                     /locus_tag="VGP27_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851849.1"
                     /translation="MVTPTLGADLLAAVARLNRLASQRARIPLPSAQGRLLSTIEDQG
                     AARISDLATLDHCSQPTMTMQVRRLEDAG"
BASE COUNT          628 a         1322 c         1268 g          530 t
ORIGIN      
        1 ggcgtcggac accaacggca acggcgactt ctcccgcaat cagttcctgc agggcaggat
       61 ggcgctgttc cagtccggca cctacaactt ggcggcgatc gccgagcaga gccgcttccg
      121 gtggggcgtc gtcccgatgc ccgtcgggcc ggtgggccgg gtgagcgtca ccaacggcat
      181 tgccgctgcg ggcaattcgg cgaccggaca tcccgacgcc gtgcggcagg tgctggactg
      241 gatgggcagc gccggcggca accaatatct cggtgcgcac ggcgcggcca tcccggccgt
      301 gctcgcggcc cagcgcgtct acttcgacta ctgggcgcgg cgcggcgtcg acgtcggccc
      361 gttcttccgg gtgctcgacg gtccgcggat acccgcgccc ggcggcccgg gattccccgc
      421 cggctaccag gcgatggaac cctacttcga cgagatgttc ctcggccgct ccgacgtggc
      481 gacaacgctg agcgatgccc aggccgccgc caacgccgcg gccgagcgct aagtcgttcc
      541 gagcagcgtc agttccagtg ccagcgcagc gccgcagacc agcagaacca gcgaaagcgc
      601 aacccagtcg cgcagtttgg gccccgacgg cgccgcggag atctggccgg ctccgccgcg
      661 cgcggtgatc gcgtcgccca tctcgtctgc acgccggagt gcgactatga cggccgctgc
      721 gatcaggtcg atgacctcga atgcccagcg acgcctgcgg gcccgccggg ttgtcagcac
      781 aggcttcgga cgcaggctgc gcgcggcata cagggtccgg aactcgtcga tcaacatcgg
      841 gaaggcgcgc agcgccagag ccagcgtgac cgcccactcg tccaccggta tccgtagccg
      901 gcgcaatggt cttccgagtt tcgcgacagc gggcgcgatc tcggcgacat tggtcgtcca
      961 cgacaccatg ccgcccagcg ccagcagcac gatcgacagc gcggtgattc gcaggaactt
     1021 cagcagtccg cccagaccga ggtcctgccc gaagagtgcg atgaccggac tgccgcccgc
     1081 caacgctgcg gtgacacctc cgagcatcag caggaaccac agccagcgag gtatcgacgg
     1141 caggacgccg cgcggaaccc gggccagcca cgccgtgatg agcacgagag ccgcgaccgc
     1201 cccgatcggc acccagccgg gatagaacgt cagcagcacg ccgatcgccg cgaccacgat
     1261 gagtttcgtt cccgcccaga ggtcatggac gaccgtgcgg ccgggcactg gccgcagcag
     1321 gacgaccggc cgccgttgtt gtctgcgtgc aggcgcggtc atgacattcc tccggcggcc
     1381 gtcgacgccg gcgccagcac accgtcctgc agatgcagga tgcgcgggca caggtcttcg
     1441 agtccggtga agtcgtgcga gatgacgatg acggtcaggc cggcgtttcg gcggagttcc
     1501 tcgagcagtc gcaacagtcc gcgcgcgctg gcggcgtcga ggccggccag tggttcgtcg
     1561 aggatgaggg cgcgcggcga tcgcgccagc agccctgcca gcaccacccg acgcatctgc
     1621 ccgccgctga gctgatcgat ccggcggtcc gccagcgccg cgtccagccc gacggtggcc
     1681 agtgcctcga tcacccgcgc acggtcgtgc ggtgagaagc cggctgccga tgcgacttcc
     1741 acatcgaccc ggctgcgcag cagctgcagt cgggcggcct ggaaggagat cgccaccgcc
     1801 cccacctgat cggacaccgg agcgccgtcg agcagacaac tgccgctcgt cggcacggca
     1861 aggcctgcca tgatccacgc cagggtggac tttcccgacc cgttcaggcc gtgaatcagc
     1921 acgccgtcgc cctcgtccac cgcgaagttg atgttccgca atgcctctgt ggcccacgga
     1981 gtagcgctgc cgtattcgtg gttcacgtcg atgagttcga gaaccggggc gtcacttcgc
     2041 ggtttgagcg ggccggtggc atccggggcc gcggtcgtct ccaccatctc ggtgttgtcg
     2101 gtcgctccgc ggtttccggt caggtcgatc gcgcggtcgg ccgagtcggc ctcgtcgttg
     2161 tagtgcgtga tgtgaaccag cgccatgcgg tggtgctcgg tcagcccgga cagcacaccc
     2221 atcagtccgt cgcggccgtc ctggtcgacc atgctggtca cctcgtcggc gatcaacagc
     2281 gacggctccc tggccagcgc cgccgccaca gcgaggcgtt gcagttcgcc gcccgacagg
     2341 ccgccggtgt cgcgctcggc caggccgtcg aggccgacct cggcgagaag ccgatgcacg
     2401 tcggtgacgg tgccggacgg cagtccccac accacgtcgt cggcgacccg ggtcccgagc
     2461 acctggctct cggggtgctg caaaatcacc gcggtgccgc cgagtcggcc cagccccacc
     2521 gagccgggac gctcgacagt gcccgaggac ggctcgcggc ccgacagcac cagcatcagc
     2581 gtggtcttgc cggacccgtt gggccccgtc accgcgacgt gctcgccggg ttcgaccgtc
     2641 agcgacaccg ggccgagtgc gtcgtggtcg ctcttcggat agcggaaccg gacgtcggtg
     2701 agccgcaccg gcaccggcgc caccgggcgg gcgtcctttg cggaatcgag cttgtgcacg
     2761 tccgggatca cgacgagccg gttgatcacc tgggacagcg cccaccagcc gatcagcatg
     2821 acgatggtga tgctgagcac cgatgacccg ccgagcagaa ccggccagta gtgaagcgcg
     2881 gtggcgaagt cgtgccgcag actctcggcg aggtggtcca agtcgggcag ccaccaccac
     2941 tggggaaaac tggtgaggac agcggcgata ccgtcgacgt tggcggtcat cgattcgaag
     3001 atcaggtgcc gcagccggac cagcaccgac agggcagcca cgacggcgcc gccgaacacc
     3061 gcaccggcga cgagtccgac gagcacgaca gtcggccatc cgcggccgcg cctgagaatg
     3121 aggccgatga gcaagccgat ataggcgcag ttgatcaccg tcatgaagcc gcccataccg
     3181 gcgatcagga acgcgatggt ggcgcccgcg accgtcgcgg cgatcagcgc ccgaacgcgg
     3241 aaccggtaaa ccagcaggcc catcggcacg ttgcccaaca gcgatacacc gccggcgaag
     3301 ggcacaacga ccgcgatgat ggcggtggct gcgcagagcg ccgccatcac cgatgagtgc
     3361 gccagctcaa tgggttgcag cgaaccccgt cggcgagtat cgctcgcacg gcgggtcgca
     3421 gtcattgcac aagtctgcca gtcgcggctc aacaatcctt tggcagcgct gtgagcgccg
     3481 acacgcggcc tgccgattga tacgcatagc caaactatgt aactatggaa tggtgactcc
     3541 gacccttggc gccgacctgc tcgccgcggt ggcccgactc aaccgcctgg cgtcgcagcg
     3601 ggcccgtata ccgctgccgt ccgcacaggg gcggctgctg tccacgatcg aggatcaggg
     3661 cgcggcgcgc atctccgatc tggccacgct cgaccactgc tcgcaaccga cgatgaccat
     3721 gcaggtgcgc cggttggagg acgcgggt
//
LOCUS       DASYLX010000447         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2652738,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000447 DASYLX010000000
VERSION     DASYLX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652738"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2341
                     /locus_tag="VGP27_10915"
     CDS             <1..2341
                     /locus_tag="VGP27_10915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEV7851850.1"
                     /translation="VTAAADLGEQCAFELALIAPQLPPFDVPDGHTEDSWLRHLVMTG
                     ARNRYGPPERARRAYEQIEHELKTIAQLNFPGYFLVVHDITRFCRESGILAQGRGSAA
                     NSAVCYALGVTNVDPVANELLFERFLSPARDGPPDIDIDIESDLREQAIQYVYDRYGR
                     DYAAQVANVITYRGRSAVRDMARALGFSQGQQDAWSKQIGQWGKLSESTHVEDVPEPV
                     IDLAMQISNLPRHMGIHSGGMVICDRPIADVCPVEWARMENRSVLQWDKDDCAAIGLV
                     KFDMLGLGMLSALHYCIDLVREHKGIDVDLAKLDLSEPAVYEMLQRADSVGVFQVESR
                     AQMATLPRLKPRVFYDLVVEVALIRPGPIQGNSVHPYIGRRNGTEPVTYDHPSMESAL
                     RKTLGVPLFQEQLMQLAVDCAGFTAAEADQLRRAMGSKRSTERMQRLRGRFYDGMRER
                     HGITGEVADRIYEKLEAFANFGFPESHSLSFASLVFYSSWFKLHHPAVFCAALLRAQP
                     MGFYSPQTLVADARRHGVTVRGPDVNASLAYATLENVGTEVRLGIGSVRHIGDELAEK
                     LVEERNTNGPFGSLLDLTGRVQLSVPQTEALATAGALGCFGITRREGLWAAGAAATQR
                     PDRLPGVGASSNVPALPGMTELELAASDVWATGISPDSFPTKFLRADLDAMGVITADR
                     LLSVPDGTRVLVAGAVTHRQRPATAQGVTFINIEDETGMVNILCSPGVWNRQRKLAQT
                     ATAMVIRGQVQNATGAVTVVADRLSKLDMRIASRSRDFR"
BASE COUNT          393 a          777 c          826 g          389 t
ORIGIN      
        1 ggtgaccgcc gcggccgacc tcggcgagca gtgtgcgttc gagctggcgc tgatcgcccc
       61 gcagttgccg ccgttcgacg ttccggacgg gcacaccgag gacagctggc tgcggcacct
      121 ggtcatgacg ggcgcaagga accggtacgg accgcccgag cgggcgcgtc gggcatacga
      181 gcagatcgag cacgagctca aaaccattgc ccagctcaac tttccgggct atttcctggt
      241 ggtgcacgac atcacccggt tctgcaggga gagcggcatc ctggctcagg gcaggggatc
      301 ggcggccaac tcggcggttt gttacgccct cggcgtgacc aacgtcgacc cggtcgccaa
      361 cgagttgctg ttcgagcggt tcctgtcccc ggcgagagac gggccgcccg atatcgacat
      421 cgacatcgaa tcggatctgc gggagcaggc cattcagtac gtctacgacc gctacggccg
      481 cgactacgcc gcgcaggtcg ccaacgtcat cacctaccgc ggccgcagcg cggtgcgtga
      541 catggcccgc gcgctgggtt tctcgcaagg gcagcaggac gcgtggagca agcagatcgg
      601 ccagtgggga aagctctccg aatcgacgca cgtcgaggat gttcccgaac cggtcatcga
      661 cctggccatg cagatctcca acctgccgcg tcacatgggc atccactcgg gtggcatggt
      721 gatctgcgac cggccgatcg ccgacgtctg cccggtggag tgggcgcgga tggagaaccg
      781 cagcgtgctg caatgggaca aggacgactg tgccgcaatc ggattggtga agttcgacat
      841 gctcggcctc ggcatgctct cggcgctgca ctactgcatc gacctggtgc gtgagcacaa
      901 gggcatcgac gtcgacctgg ccaagctgga tctgtccgag cccgcggtgt acgagatgct
      961 gcagcgcgcc gactccgtcg gtgtgttcca ggtggagtcg cgtgcgcaga tggccacgct
     1021 gccgcggctg aagccgcggg tgttctacga cctggtcgtc gaggtggcgc tgatcaggcc
     1081 cggcccgatc cagggcaact cggtgcaccc ctacatcggc aggcgcaacg gcaccgaacc
     1141 ggtgacctac gaccatccgt cgatggagtc tgcgctgcga aaaacgttgg gtgtgcctct
     1201 gtttcaggag cagctcatgc agctcgcggt cgactgcgcc ggcttcaccg ccgccgaggc
     1261 cgaccagttg cgtcgggcga tgggatccaa gcggtcgacc gagcggatgc agaggttgcg
     1321 cgggcggttc tacgacggta tgcgcgaacg gcacggcatc accggtgagg tggccgaccg
     1381 gatctacgag aagctggaag cgttcgccaa cttcggtttt ccggagagcc attcgctgtc
     1441 cttcgcctcg ctggtgttct actcgtcgtg gttcaagctg caccaccccg cggtgttctg
     1501 cgccgcgctg ctgcgggctc agccgatggg cttctactca ccgcagacct tggtggccga
     1561 tgcccgtagg cacggagtca ccgtgcgtgg accggacgtc aacgccagcc tggcgtacgc
     1621 gacgctggag aatgtcggca ccgaggtgcg gctgggcatc ggcagcgtcc gccacatcgg
     1681 tgacgagctt gccgagaagc tcgtcgaaga acgaaacacc aacgggccgt tcggttctct
     1741 gttggatctg accggacggg tgcagctctc ggtcccgcag accgaagcgc tggcgacggc
     1801 gggggcgctg ggctgcttcg ggatcacccg ccgcgaaggg ttgtgggccg cgggcgccgc
     1861 cgccactcag cgaccggacc ggctgcccgg ggtcggggcg tcgtcgaacg tcccggccct
     1921 gccgggtatg accgagctgg aactggcggc ctccgacgtt tgggccaccg ggatttcgcc
     1981 cgacagcttt cccaccaagt tcctgcgggc ggacctcgac gcgatggggg tcatcacggc
     2041 cgaccggctg ctttcggtgc ccgacggaac acgggtgctg gtggcggggg cggtgacaca
     2101 ccggcagcgt cccgcgacgg cgcagggggt gacgttcatc aacatcgagg acgagaccgg
     2161 catggtcaac atcctgtgct caccgggggt gtggaaccgt cagcgcaagc tggcgcagac
     2221 ggcgacggcg atggtgatcc gcggccaggt ccagaacgcc accggcgcgg tcaccgtcgt
     2281 cgccgaccgg ctcagcaagc tcgacatgcg gatcgcctcc cgcagccgcg atttccgctg
     2341 acccacatct ccgcgacagt gcgggtctgc acgcagacac tccgc
//
LOCUS       DASYLX010000448         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3859909,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000448 DASYLX010000000
VERSION     DASYLX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3859909"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VGP27_10920"
     CDS             <1..1384
                     /locus_tag="VGP27_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7851851.1"
                     /translation="ANSVIARNSGRREKRLWTDAGEGELIVGYVADNEHDEARFVAEE
                     IDALVDGARGAGDITYNDVAVFYRTNNSSRALEEVFIRAGIPYKVVGGVRFYERREIR
                     DIVAYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVAVYAENTGVSFNDALQAAAEG
                     KVPMLNTRAEKCIAGFVEMLDELRGRLDGELGELVEAVLDRTGYRTELEASSDPQDLA
                     RLDNLNELVSVAHEFSIDLANAEALGTDLDDEDVPDTGVLAAFLERVSLVADADELPE
                     HGAGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPTELSEERRLAYVGITRA
                     RQRLYVSRAKVRSSWGQPMLNPESRFLREIPQELIEWRRIDPAPSLSAPVSGAGRFGT
                     PRPSPARASAGKRPLLVLEPGDRVNHDKYGLGRVEEVSGMGESAMSLIDFGSAGRVKL
                     MHNHAPVAKL"
     gene            complement(1463..>1648)
                     /locus_tag="VGP27_10925"
     CDS             complement(1463..>1648)
                     /locus_tag="VGP27_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851852.1"
                     /translation="LLRRRMARLLVVVGSVIGLLIFASLFIAGAKLAPIVYAIPALPV
                     ITIALALLPATARWAHP"
BASE COUNT          293 a          515 c          596 g          244 t
ORIGIN      
        1 ggccaactcc gtcatcgccc gcaacagcgg gcgccgtgaa aagcggttgt ggaccgacgc
       61 gggcgaaggc gaactcatcg tcggatacgt cgccgacaac gagcacgacg aggcgcggtt
      121 cgtcgcagag gagatcgatg cgcttgtcga cggtgctcgt ggggcgggcg acatcaccta
      181 caacgacgtc gcggtgttct accgcaccaa caactcgtca cgcgccttgg aagaggtgtt
      241 catccgcgcg ggcatcccgt acaaagtggt tgggggcgtt cgcttttacg agcgcagaga
      301 gatccgcgac atcgtcgcct acctgagggt gctggacaac cccggcgacg cggtgagcat
      361 gcggcgcatc ctgaacaccc cgcgacgggg aatcggcgac cgggcggagg cgtgtgtcgc
      421 ggtatacgcc gagaacaccg gcgtcagctt caacgacgcg ctgcaggcgg ccgccgaagg
      481 caaggtgccg atgctgaaca cgcgcgccga gaagtgcatc gccggtttcg tcgagatgct
      541 cgacgagctg cgcgggcgtc tcgacggcga gctgggggag ctggtcgagg cggtgctgga
      601 ccgcacgggg tatcgcaccg agctggaggc gtccagcgac ccgcaggatc tggcacggct
      661 ggacaacctg aatgaattgg tcagcgtcgc acacgaattc agcattgacc tggccaacgc
      721 cgaggcgctg ggaaccgatc tcgacgacga agatgtgccc gacaccggcg tgctggcggc
      781 gttcctggag cgggtgtcgc tggttgccga cgccgacgaa ctgcccgagc acggcgcggg
      841 cgtggtgacg atgatgacgc tgcacaccgc caaggggctg gagttccccg tcgtgttcgt
      901 gacagggtgg gaggacggca tgttcccgca catgcgggcg ctgggcgatc ccaccgagct
      961 gtcggaggaa cggcggctgg cctacgtcgg cataacccgt gcgcgccaac ggctttacgt
     1021 gagccgggcg aaggtccgct cgtcgtgggg ccagccgatg ctgaaccccg agtcgcggtt
     1081 ccttcgcgag attccacagg agctgatcga gtggcggcgc atcgatcccg cgccgtcgtt
     1141 gtcggcgccc gtaagcgggg cgggccgctt cggcacaccg cggccgtcgc ccgcacgcgc
     1201 ctcggcaggc aagcgaccgc tgctcgtgct ggaaccaggc gaccgggtca accacgacaa
     1261 gtacgggctc ggccgcgtcg aggaggtgtc gggcatgggg gaatccgcga tgtcgctcat
     1321 cgacttcggc agtgcgggcc gggtgaagct gatgcacaat cacgcgccgg tggccaagct
     1381 ctagccgctc cactctcgcc gagatcgacg aaatggcgcg acttactcgc acttttgcgc
     1441 ccaagtgttg gtttgggcgt agttaagggt gggcccagcg ggcggtcgcg ggcagcagcg
     1501 cgagcgcgat cgtgatcacc ggcagggcgg gaatcgcgta gacgatcggc gccagtttcg
     1561 cgcccgcgat gaacaggctc gcgaagatca acagcccgat caccgagccc acgacgacga
     1621 gcaggcgggc catccggcgc cgaagcag
//
LOCUS       DASYLX010000449         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2434036,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000449 DASYLX010000000
VERSION     DASYLX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434036"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGP27_10930"
     CDS             complement(<1..330)
                     /locus_tag="VGP27_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEV7851853.1"
                     /translation="MTTAASGVGVATIAADGTVLDAWFPAPELAGDGPSGTVRLSVAE
                     VPPELAELAGRDEDRDVEVVVVRTVIASLDDKPADTYDAYLRLHLLSHRLIQPHGASV
                     DGIFAVLA"
     gene            complement(327..968)
                     /locus_tag="VGP27_10935"
     CDS             complement(327..968)
                     /locus_tag="VGP27_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851854.1"
                     /translation="MITTQPSAGTRRSVRARLLDAANELFYDEGVQTVGIDRIIERAG
                     VAKGSLYNTFGSKDELVWAYLQSRHAQTIQRLTQAVEKHTDPVDRLLAVFDAQAEIFA
                     QPDFRGCAFVSASAESPLGGLVHQAADAYRHDIRSLFVRLADEAGAPDPTALGHQLHL
                     IYDGAGIGARMDRDPSAALRARAAAASLLDTALREKHWVRVASTSVMRTRGAR"
     gene            complement(965..1417)
                     /locus_tag="VGP27_10940"
     CDS             complement(965..1417)
                     /locus_tag="VGP27_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7851855.1"
                     /translation="MTTDCYRPSASAQVRDQVELYEATDGREGGTPEGRPVIILTTVG
                     ARTGSIRKNPVMRIKDGDTYVAVASDGGAVTHPAWYRNIVADPEISLQDCAIVRQVRA
                     REVHGEEKARWWRIAERDWPHFPEYRAKAGDREIPLLLLERKREDGDR"
     gene            complement(1417..2142)
                     /locus_tag="VGP27_10945"
     CDS             complement(1417..2142)
                     /locus_tag="VGP27_10945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851856.1"
                     /translation="MTQESRPVALVTGATSGIGRAVALDLARDGFDVIVHGRDAARGT
                     ATVKEIEAEGGQARFVGAELGNADAVAALAEQAGEVEVLVNNGGFSWFGPSAELDTDT
                     FDDLFSSNVRAAYQLVAALAPGMAARGHGSIISVDSMAGRVGLAGGAAYGATKAALTA
                     MSRAWAAEFSPSGVRVNTVAPGPVFTDDSKRDLIENLAATTLFKRGAQPEEVSGVIAF
                     LASPRASYITGAVIPVDGGRTAI"
     gene            2223..3290
                     /gene="dapE"
                     /locus_tag="VGP27_10950"
     CDS             2223..3290
                     /gene="dapE"
                     /locus_tag="VGP27_10950"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEV7851857.1"
                     /translation="MGLDLRGDPIALTAALVDIPSESRDEQRIADEVEAALREQTNRF
                     DILRNGNTVLARTDYGLPSRVILAGHLDTVPIADNVPSRLTDGELHGCGTSDMKSGDA
                     VFLHLAATVDDPAHDITLIMYDCEEIEAAANSLGRIERELPDWLHADVAILGEPSGGF
                     VEAGCQGTLRVVVSADGTRAHSARSWLGDNAIHKLGAVLDRLRSYEARSVDIDGCVYR
                     EGLSAVRIDGGVAGNVIPDAASVAINFRFAPDRSVEQAGKHVHDVLDGLDVHIEQTDA
                     APGALPGLTEPAAAALVDAADGQVRAKYGWTDVARFAALGVPAVNFGPGDPNLAHRVD
                     ERVEVRQITAATDMLRRYLTA"
BASE COUNT          525 a         1117 c         1110 g          562 t
ORIGIN      
        1 cgccaggacg gcgaagatgc cgtcgacgct ggcaccgtgc ggctggatca gccgatggga
       61 aagcaggtgc agccgcagat aggcgtcgta ggtgtcggcg ggcttgtcgt ccagcgaggc
      121 gatcaccgtg cgcaccacga cgacctcgac gtcacggtcc tcgtcgcggc ctgcgagctc
      181 ggccagctcg gggggcacct cggccaccga cagccgcacc gtgcccgacg ggccgtcgcc
      241 ggccagctcg ggagccggga accacgcgtc gaggacggtt ccatcggcgg cgatggtcgc
      301 cacgccgacg ccagaagctg cagttgtcat cttgctcctc gcgtccgcat cacgctggtt
      361 gaggctactc gaacccagtg cttctctcgg agcgcggtgt cgaggaggga agcggctgcg
      421 gcgcgggcac ggagtgcagc ggagggatcg cgatccatcc gagcgccgat tccggcgccg
      481 tcgtagatga ggtggagttg atggcccagc gcagtggggt cgggggctcc ggcttcgtcg
      541 gctaggcgca cgaacaaact gcggatgtcg tgccggtacg cgtcggcggc ttgatggacc
      601 agtcccccca gtggcgactc ggcgctggcc gacacgaagg cacagccgcg gaagtccggc
      661 tgcgcgaaga tctctgcttg ggcgtcgaac accgcgagca gccggtcaac cggatctgtg
      721 tgcttttcga cggcctgggt gagtcgctga atggtctgcg cgtggcgcga ctgcaggtag
      781 gcccaaacga gctcgtcctt gctaccgaac gtgttgtaga gcgaaccttt tgcgactcca
      841 gctcgctcaa tgatccgatc gatacctacc gtctggacgc cttcgtcgta gaagagttcg
      901 ttggcggcgt ccaacaaccg ggcccggacg gaacggcggg tgccggcact cggttgggtg
      961 gtgatcatcg gtcgccgtcc tcccgcttcc gctcgagcaa cagcagggga atttctcgat
     1021 cgccggcttt ggctcggtac tcggggaagt gcggccaatc gcgttcggcg atccgccacc
     1081 accgcgcctt ctcctcgcca tgcacttctc gggcccgcac ctggcgcacg atcgcgcaat
     1141 cctgcagaga tatctcggga tcggcgacga tgttgcggta ccaagccgga tgcgtgacgg
     1201 cccctccgtc ggatgccacg gcaacgtagg tgtcgccatc cttgatccgc atgacggggt
     1261 tcttcctgat gctgccggtc ctggcgccga cggtggtcag gatgatcacg ggccggccct
     1321 ccggggtgcc accttcacgt ccgtcggttg cctcgtacag ctccacctga tcgcggacct
     1381 gcgcgctggc gctgggccgg tagcagtctg tcgtcatcag atcgccgtcc gtccaccgtc
     1441 gacagggatg acggcgccgg tgatgtagct ggcccgcggc gaagcgagga aggcgatgac
     1501 cccggagacc tcctccggtt gcgcaccgcg cttgaacaag gtagtcgccg ccaggttctc
     1561 gatgagatcg cgcttggaat cgtcggtgaa gaccgggccg ggcgccacgg tgttgacgcg
     1621 gacacccgac gggctgaact cggcggccca ggcgcggctc atcgcggtca gcgcggcctt
     1681 ggtcgcgccg tatgcggcgc ccccggcaag gccgacgcga ccggccatgc tgtcgacgct
     1741 gatgatgctg ccgtgtccgc gggccgccat tccgggggcc agcgccgcaa cgagctgata
     1801 cgcggcacgc acattgctgc tgaacagatc gtcgaacgtg tctgtgtcga gctcggccga
     1861 gggtccgaac caggagaatc cgccgttgtt gacaagcacc tccacttcgc ccgcctgttc
     1921 tgcgagagca gcgacggcgt cggcgttgcc gagctcagcc ccgacgaatc gcgcctgccc
     1981 gccttcggct tcgatctcct ttaccgttgc ggtgccgcga gcggcgtcgc gcccgtgcac
     2041 gatcacgtca aacccgtctc gcgccaaatc gagcgccacc gccctgccga tacccgacgt
     2101 cgcgccggtc accagcgcga ccggcctgct ttcctgtgtc atctccgact cctgtctgtc
     2161 tataagcact aagcgcgtta gacaggtctg tctattcctg agccaccatc tacccttcac
     2221 gtgtgggcct ggatttacgt ggcgatccga tcgcgctgac cgcggcgctg gtggacattc
     2281 ccagcgagtc gcgcgacgag cagcgcatcg ccgacgaggt cgaggccgcg ctgcgcgagc
     2341 agacgaaccg gttcgacatc cttcgaaacg gcaacaccgt gctggcccgc accgattacg
     2401 gactgccgtc gcgggtcatc ctcgccggcc acctcgacac cgttccgatc gccgacaacg
     2461 tgcccagccg cctcactgac ggcgaattgc acggctgcgg tacctcggat atgaagtcgg
     2521 gggacgcggt gttcctgcac ctggccgcca ccgtcgacga ccctgcccac gacatcacgc
     2581 tgatcatgta cgactgcgag gaaatcgaag ccgccgcaaa cagtttgggc cgcatcgaac
     2641 gcgagttacc ggactggttg catgccgacg tggcgatcct cggcgaaccg tccggcggat
     2701 tcgtcgaggc cggctgtcag ggcacgctgc gtgtcgtcgt cagcgccgac ggcacccggg
     2761 cgcattcggc gcgatcgtgg ttgggcgaca acgcaattca caaactgggc gctgtgctcg
     2821 accggctgag gtcctacgaa gcccgtagcg tggacatcga cgggtgtgtc taccgcgagg
     2881 ggctgtcggc cgtgcgcatc gacggcggtg tggcgggcaa cgtcatcccc gacgcggcct
     2941 cggtggccat caacttccgc ttcgcaccgg accgcagtgt cgagcaggcc ggcaaacacg
     3001 ttcacgacgt gctcgacggt ctcgacgttc atatcgagca gaccgacgcc gcccccggcg
     3061 ccctgccagg gttgaccgaa cctgcggccg ccgcgttggt cgatgccgcc gacggtcagg
     3121 tgcgggcgaa gtacgggtgg acggatgtgg cccggttcgc ggcgctcggc gtgcccgccg
     3181 tcaacttcgg acccggcgat ccgaacctcg cgcaccgcgt cgacgagcgg gtcgaggtgc
     3241 gccagatcac cgcggccacc gacatgctgc ggcgatattt aacggcttga ctcgacgcat
     3301 agcctgaagg catg
//
LOCUS       DASYLX010000450         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_679595,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000450 DASYLX010000000
VERSION     DASYLX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679595"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..972
                     /locus_tag="VGP27_10955"
     CDS             79..972
                     /locus_tag="VGP27_10955"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV7851858.1"
                     /translation="MRPALSDYQHVASGKVREIYRIDDDHLLFVASDRISAFDYILDN
                     EIPDKGRILTAMSVFFFDYTEAPNHLAGPADDARIPDEVLGRALVVRKLDMIPVECVA
                     RGYLTGSGLLDYQKTGEVSGIALPPGLTEGSKFDFPLFTPATKAELGEHDENISFADV
                     IELVGAERANQLRDLTLQTYLRAAEHALTKGIIIADTKFEFGVDERGHLVLADEVFTP
                     DSSRYWAVETYREGVVAPSFDKQFVRNWLLSPESGWDRGGDEPPPQLPPTIVDATRER
                     YINAYERISGLQFADWIGPQA"
     gene            969..>2917
                     /locus_tag="VGP27_10960"
     CDS             969..>2917
                     /locus_tag="VGP27_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV7851859.1"
                     /translation="MTAPVPPIAKRIEHRREHHGDVFVDPYEWLRDKSDPAVIEHLEA
                     ENAYTEQITDHLAPLRQKIFDEIKARTKETDLSVPVRRNDWWYYTRSFEGKQYSVHCR
                     CPITHPDDWTPPQFDEHTEVPGEQILLDENALAEGHDYFALGAASVSVDAHTLAYSVD
                     VLGDERYTLRFKDLRTGRLYDDEIVGIGAGATWAADNRTVYYTTVDDAWRPDTVWRHR
                     VGAGLPAEKVYHEPDEKFWLAVGRTRSDRYVFIAAGSAVTSEIRYADATDGHAEFRPV
                     WPRREGVEYSVEHAVVGGEDRFLILHNDGAENFTLVEAPVSDPTATRTLIEHRDDVRL
                     DAVDAFAGHLVVSYRSEALPRIQLWPLYADGEYGRPEDFTFESELMSAGLSGNPNWDS
                     PKLRIGATSFVTPVRIYDVDLATGERTLLREQPVLGDYRPEDYVERRDWATATDGARV
                     PVSIIHRVGLQFPAPTLLYGYGAYESCEDPRFSIARLSLLDRGMVFAIAHVRGGGELG
                     RPWYEHGKLLEKANTFTDFVAVAAHLIKSDVTRPENLVALGGSAGGLLIGAVANMAPQ
                     LFAGFLAQVPFVDALTTILDPSLPLTVTEWDEWGNPLADENVYHYMKSYSPYENVGAK
                     DYPPILAMTSLNDTRVYYVEPAKWVA"
BASE COUNT          529 a         1001 c          915 g          472 t
ORIGIN      
        1 gcgggcgtgg tggcgtaggg cacatacctc gatgtcatgc cgtcgatccc agcacagatc
       61 gaccgctagg ctggcccgat gcgcccagct ctgtccgact accagcacgt ggccagtggc
      121 aaggttcgcg agatctaccg aattgatgat gaccacctgc tgttcgtcgc cagcgaccgc
      181 atttcggcgt tcgactacat cctcgacaac gaaatacccg acaagggtcg catcctgacc
      241 gcgatgagcg tgttcttctt cgactacacc gaggcgccca accacctcgc gggcccggcc
      301 gacgacgcgc gcatccccga cgaggtgctc ggccgcgcgc tggtcgtgcg caagctcgac
      361 atgattccgg tcgagtgtgt cgcccgcggc tacctgacgg gttcgggcct gctggactac
      421 cagaagaccg gcgaggtcag cggcatcgca ttgccgccgg gactgaccga aggcagcaag
      481 ttcgacttcc cgctgttcac gccggccacc aaggccgaac tcggcgagca cgacgagaac
      541 atctcgttcg ccgacgtgat cgagctggtc ggcgccgagc gcgccaacca actgcgcgat
      601 ctcacactgc agacctacct ccgcgccgcc gagcacgcct tgacgaaagg gatcatcatc
      661 gccgacacca aattcgagtt cggcgtcgat gagcgcggac acctcgtgct tgccgatgag
      721 gtgttcacgc ccgactcgtc gcggtactgg gccgtcgaga cctaccggga gggtgtcgta
      781 gcgcccagct tcgacaagca gttcgtccgc aactggctgt tgagcccgga atcgggctgg
      841 gaccgcggcg gcgacgaacc gccgccccag ctgccgccca ccatcgtgga cgccacccgt
      901 gagcgctata tcaatgccta cgagcgcatt tcgggcctgc agttcgccga ctggatcgga
      961 cctcaggcat gaccgctccg gtaccgccca tcgcgaagcg catcgaacac cgccgcgaac
     1021 atcacggcga cgtcttcgtc gacccctacg agtggctgcg ggacaagtcc gatcccgcgg
     1081 tgatcgaaca cctcgaggcg gagaacgcct acaccgagca gatcaccgac cacctggcgc
     1141 cgctacggca gaagatcttc gacgagatca aggcccgcac caaggagacc gacctgtcgg
     1201 tgccggtccg gcgcaacgac tggtggtatt acacgagaag tttcgagggc aagcagtaca
     1261 gcgtgcactg ccgttgtccg ataacccatc ccgacgactg gacacccccg cagttcgacg
     1321 agcacaccga ggttccgggc gagcagatcc tgctggacga gaacgcgctg gccgaaggcc
     1381 acgactactt cgcgttgggc gccgcctcgg tcagcgtcga cgcccacaca ttggcgtatt
     1441 cggtcgacgt cctcggtgac gagcgataca cgttgcggtt caaagacttg cgcaccggtc
     1501 ggctctacga cgacgaaatc gtcggcatcg gcgcaggcgc gacatgggcc gccgacaacc
     1561 ggaccgtcta ctacacgacg gtggacgacg cgtggcggcc ggacacagtt tggcggcacc
     1621 gtgtcggagc gggcctgccc gccgagaagg tctaccacga gcccgatgag aagttctggc
     1681 tcgcggtggg acgaacccgc agcgacaggt acgtcttcat cgccgccgga agtgccgtca
     1741 cgtcggagat ccgctacgcc gacgccaccg atggacacgc cgagttccgt ccggtgtggc
     1801 cgcggcgcga aggcgtcgag tactccgtcg aacacgcggt tgtcggcggt gaggaccggt
     1861 ttctcatcct gcacaacgac ggcgcggaga atttcaccct cgtcgaggcg ccggtgagcg
     1921 atccgaccgc cacgcgcacg ctcatcgagc accgcgacga cgtccgtctc gacgccgtcg
     1981 acgcgttcgc cgggcacctg gtggtgagct accgcagcga ggcgctgccg cgaattcagc
     2041 tgtggccgct ctacgctgac ggcgagtacg gtcgccccga agacttcacc ttcgaatccg
     2101 agctgatgtc ggcgggcctg agtggcaacc ccaactggga ctcgcccaaa ctgcgaatcg
     2161 gtgcgacgtc gttcgtcact ccggtgcgca tctacgacgt cgacctcgcc actggcgagc
     2221 ggacgctgct gcgcgaacaa ccggtgctcg gcgactaccg ccccgaggac tacgtcgagc
     2281 ggcgtgactg ggcgaccgcc acggacgggg cccgggtccc ggtgtcgatc atccaccgcg
     2341 tcggcctgca attcccggcg cccacactgc tttacggcta cggcgcctac gagtcgtgcg
     2401 aggatccgcg gttctcgatc gcccggttgt cgctgctcga ccgcggcatg gtgttcgcca
     2461 tcgcccacgt gcgtggtggc ggcgagctcg gccgaccgtg gtacgagcac ggcaagctgc
     2521 tggagaaggc gaacaccttc accgacttcg tcgccgtcgc agcgcatctc atcaagtccg
     2581 acgtgacccg acccgagaac ctcgtcgcgc tcggcggcag tgcgggcgga ctgctcattg
     2641 gtgccgtggc caacatggcg ccgcagttgt tcgccggctt cctggctcag gtgccgttcg
     2701 tcgatgcgct gacgacgatc ctcgacccgt cgcttccgct caccgtcacc gagtgggacg
     2761 agtggggaaa ccccctggcc gatgagaacg tctaccacta catgaagtcc tactcgccgt
     2821 atgagaacgt cggtgccaag gactacccgc cgatcctggc gatgacatca ctcaacgaca
     2881 cccgggttta ctatgtcgag cccgcgaaat gggttgc
//
LOCUS       DASYLX010000451         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2434152,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000451 DASYLX010000000
VERSION     DASYLX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434152"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGP27_10965"
     CDS             complement(<1..597)
                     /locus_tag="VGP27_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEV7851860.1"
                     /translation="MGWNSWNSGIPLTEQTVRQTIDAMVSSGLRDAGYRYVNLDAGWA
                     APTRDHDGELRADPDRFPHGIAALARYAHDRNMYLGLYHSPFNEGCGQDPRIGGAGHE
                     RVDAKTFADWGVDYLKYDWCRADASHELQVRYFSAMRDALRASGRRIFYSVNPNSSAD
                     AGAGVRYDWSKIADMNRNSVDLVPAWGDDDLWSRGLAGV"
     gene            960..1352
                     /locus_tag="VGP27_10970"
     CDS             960..1352
                     /locus_tag="VGP27_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851861.1"
                     /translation="MQDRAVFAVIADLGRKPHRRHISAAALEIHVRTVARSVHTECPA
                     FIGYTDLDAIAPGPTTTMAALELCLANLWIDARDGYVIADSELIEQLVAGPSHLWFRR
                     TASQLSRATLRALRKVWKALNEERFIPF"
     gene            complement(1424..2242)
                     /locus_tag="VGP27_10975"
     CDS             complement(1424..2242)
                     /locus_tag="VGP27_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7851862.1"
                     /translation="MNSLDGRVALITGAARGMGRAHAVKLAGEGADVIAVDICADFAS
                     TDYPGSTADDLAETGRLVESHGRRILVGRVDVRDGEAMRAVVAEGLREFGRLDIVVAN
                     AGIIRVTDCEDREQTFREIVDVNLVGAWNTVDAAIPALIDGGRGGSIVLTSSTQGIKA
                     LGTDRAGPQAYAASKRALVGLMQGWATDLARHSIRVNTIHPSGVATDMILNDATMALV
                     AANDPWIVAQQNALPISLLQPEDVAGAVAWLVSDAGKFVTGTSWPLDAGFLLRN"
     gene            complement(2239..3105)
                     /locus_tag="VGP27_10980"
     CDS             complement(2239..3105)
                     /locus_tag="VGP27_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851863.1"
                     /translation="MARWFITGCSTGIGREIARAALEAGHSAVVTARRTETVADFADT
                     FGDRAAIVALDVTDKDQIATAVRAADDAFGGIDVLVNNAGNGYLSAIEEGEDDKVRKL
                     FDTNYFGVVDTIKAVLPQMRARQSGHIVNISSMTGLVANPPNAYYSSTKFALEALTEA
                     LAQEVKPLGIKVCAIEPGAFRTDWAARSMWESATPIVDYEENVGARKTLIKEFANHLP
                     GDPRKVAEAVMMMTNLDEPPLRLLLGRDVLKAVRDKLAAFSASIDEWESVTRDVNFKT
                     ADARSTEGVTDE"
     gene            complement(3107..4360)
                     /locus_tag="VGP27_10985"
     CDS             complement(3107..4360)
                     /locus_tag="VGP27_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851864.1"
                     /translation="MSDFTGDPVATASQHEQELAALELTEHATVKAAYRSVAETWLSR
                     AKASDAMRERFDAAFTEVMFSAAVWSSNQDKLRPKVSCITRIGHPVAGRTIPGSRWGI
                     DNPDSVYRVIPVSADERYEIRGRVGANRMTENYFTLWDANMGTVAVLNGRTMEVDSAG
                     SFTITVDSDPAGGRANHVQTTPAAHEFYIRDVLLNWDRDDPNHLSVQRLGPATTTPPR
                     TVDEQADATADMMAYFANFTGKLSHGVYKMAPNNFNLAWSADRVGAMRNQVYVMGRFD
                     LNPGEAFVVDMSDGGAEYFTVPLSNIWGTTLDIVGRTGSLNKAQSVPNEDGTYTYVIS
                     PVDPGLANWIDSDGLNEGILTLRMAEFGGDGPNEDLGARGRVVKLEDLDNELPTARRV
                     SAQDRAAELADRRAAYLRRLPEGTN"
BASE COUNT          735 a         1482 c         1488 g          814 t
ORIGIN      
        1 gacaccggcg agcccccttg accacaggtc gtcgtcgccc cacgcgggca cgagatcgac
       61 cgagttgcgg ttcatgtcgg cgatcttcga ccagtcgtat ctaacgccgg cgcccgcgtc
      121 ggccgaactg ttcgggttga cactgtagaa gatgcgacgg ccgctggcgc gcagcgcatc
      181 tcgcatcgcc gagaagtagc gcacctgcaa ctcatgagac gcatcggcgc gacaccagtc
      241 gtatttgagg tagtcgacac cccagtcggc gaacgtcttc gcgtcgacgc gctcgtggcc
      301 ggccccaccg atgcgcgggt cctggccgca gccttcgttg aacgggctgt ggtagagacc
      361 cagatacatg ttccggtcat gggcgtagcg ggcaagcgcg gcgatgccgt gcgggaagcg
      421 atcgggatcc gccctcaact cgccgtcgtg gtcacgggtc ggcgcggccc agccggcgtc
      481 gaggttgaca tagcggtagc cggcatcgcg cagacccgac gacaccatgg catcgatggt
      541 ttggcggacc gtctgctcgg tcagcgggat ccccgagttc caggaattcc agcccatcgg
      601 cggaagcatg ggcgcccgac tgggaggggc cccgcagccg gcggcgatga caccagcgca
      661 gacggccaga atcaggagcc gacgcacatc gtcagagtga catcacttcc tggcaataac
      721 gtttgagcgc ctgtgcccag cgaagccggc ggtccggctt cggcggaccc acacacgctg
      781 ccactcccga ggacctgcgc cgcctcagct ggtagcaaac gctcgagggc gcctggtctg
      841 ccctggtcgc ctgagtcgaa ttcggtgcgt ggatgcgccc gaacggaatt cgaaagcgag
      901 ccgtgggcaa accgattgtt aactcgacat tacacgcgtg tcgggagtac cttcgtaccg
      961 tgcaggacag agccgtcttc gccgtaatcg ccgacctggg acgcaaaccc caccgacgac
     1021 acatctctgc cgccgccttg gaaattcacg tccggaccgt cgcgcgaagc gtccacaccg
     1081 agtgtccggc cttcatcggc tacaccgacc tcgacgcgat tgcacctggt ccgaccacca
     1141 cgatggccgc actcgaactg tgccttgcga atctatggat agacgccagg gacggctatg
     1201 tcatcgccga cagcgagctg atcgagcagc tggtggccgg cccttcgcat ttatggtttc
     1261 ggcgcacggc gtcgcagctg tcacgagcca cgctcagggc gttgcggaag gtatggaaag
     1321 cgctcaacga ggagcggttc attccgttct agcccgttgt ccgggccgac aagcccaacc
     1381 gcagtgacgg gtcgtgtgtc gtcggccagt aacccgaagc cggtcaattg cgcagcagga
     1441 agcccgcgtc gagtggccac gaggtgcccg tgacgaactt gcctgcgtcg gagaccagcc
     1501 acgccaccgc tcccgcgacg tcttccggct gcagaaggga aatgggcaaa gcgttctgtt
     1561 gggcgacgat ccagggatcg ttggccgcga cgagcgccat tgtcgcgtcg ttgaggatca
     1621 tgtcggtcgc gacaccgctc gggtgaatgg tgttgacgcg gatcgagtgc cgcgcgaggt
     1681 cggtcgccca cccttgcatg aggcccacca gggcccgctt ggacgccgcg tacgcctgcg
     1741 gtcccgctcg gtcggtgccc aacgccttga tcccctgtgt cgaactcgtc aggacgatgg
     1801 acccaccccg tccgccgtcg atcagcgcgg ggatcgccgc gtcgacggtg ttccacgcgc
     1861 cgacgaggtt cacgtccacg atctcgcgga acgtctgctc acggtcttcg cagtcggtga
     1921 cccggatgat gccggcattc gccaccacga tgtcgaggcg accgaactca cgcaggccct
     1981 ccgcgaccac agcccgcatg gcttcgccat cgcgaacatc gacccttccc accaggattc
     2041 gtcggccatg gctctcgacc agtcgtccgg tctccgccag gtcgtcggcg gtcgagccgg
     2101 ggtagtcggt ggacgcgaag tctgcacaaa tgtcgacggc gatgacatca gcgccctcgc
     2161 cggccagctt cacggcgtgc gcgcggccca tgccgcgtgc tgccccggtg atcaaggcga
     2221 cgcggccgtc gagactattc actcgtcagt cacgccttcg gtgctcctcg cgtccgcagt
     2281 cttgaagttg acgtccctgg tgacggattc ccactcgtcg atcgatgccg agaaggcggc
     2341 cagtttgtca cgcaccgctt tcaggacgtc gcgaccgagc agcagcctca acggcggttc
     2401 gtcgaggttg gtcatcatca tcaccgcctc cgccaccttg cgggggtcac cgggaaggtg
     2461 gttggcgaat tccttgatca gcgtctttcg tgcgccgacg ttctcctcgt aatcgactat
     2521 cggagtggcg gattcccaca tcgaccgggc tgcccagtcg gtgcggaagg cacccggctc
     2581 gatggcgcac accttgatcc ccagcggctt cacttcctgc gccagcgctt cggtcaatgc
     2641 ctcgagtgca aacttggtcg aggagtagta cgcgttcggc gggttggcca ccagaccggt
     2701 catcgacgag atgttgacga tgtggcccga ttggcgggcg cgcatctgtg gcagcaccgc
     2761 cttgatggtg tccacgacgc cgaagtagtt ggtgtcgaac agtttacgaa ccttgtcgtc
     2821 ctcgccctct tcgatcgccg agaggtagcc gttgcccgcg ttgttgacca ggacgtcgat
     2881 cccaccgaat gcatcgtctg ccgcccggac ggccgtggcg atctgatcct tgtcggtgac
     2941 gtcgagcgcg acgatggccg cgcgatcgcc gaaggtgtcg gcgaaatcgg cgacggtctc
     3001 ggtgcgccgt gccgtcacca ccgccgagtg tcctgcttcc aatgcggcac gggcaatttc
     3061 gcgtccgatg ccggtggagc agccggtgat gaaccagcga gccatatcag ttcgtccctt
     3121 cgggcagccg acgcagatac gcggcgcgtc ggtcggcgag ttcggctgcg cggtcctggg
     3181 cggatacccg gcgcgctgta ggaagttcgt tgtcgaggtc ctcgagtttc accactcggc
     3241 cgcgggcgcc gaggtcctcg ttcggtccgt cgccgccgaa ctcggccatc cgcagagtca
     3301 ggatgccctc gttgagaccg tccgagtcga tccagttggc caggccgggg tcgacggggg
     3361 agatgacgta ggtgtacgtg ccgtcctcgt tgggcaccga ctgcgccttg ttgaggctgc
     3421 ctgtgcggcc gacgatatcc agcgtggtgc cccagatgtt gctcagcgga acggtgaagt
     3481 actcggcccc gccgtcgctc atgtcgacga cgaaggcctc gccgggattg agatcgaacc
     3541 ggcccatcac gtacacctgg ttgcgcatgg cgccgacccg gtcggccgac catgcgaggt
     3601 tgaagttgtt gggtgccatc ttgtagaccc cgtggctgag cttgccggtg aagttcgcga
     3661 agtaggccat catgtccgcg gtggcgtcgg cctgttcgtc gacggtgcgg ggcggcgtcg
     3721 tcgttgcagg gcccagccgt tgcacgctca gatggttcgg gtcgtcgcgg tcccagttga
     3781 gcagcacgtc tcggatgtag aactcgtgcg ccgccggcgt cgtctgcaca tggttggccc
     3841 ggccaccggc agggtcggag tcgacggtga tggtgaacga tccggcgctg tccacctcca
     3901 tggtgcggcc gttgagcaca gcaaccgtgc ccatgttggc atcccacagc gtgaagtagt
     3961 tctccgtcat ccggttcgcg cccacccgac cgcggatctc gtagcgctcg tcggcggaga
     4021 cgggaatgac gcggtagacg ctgtcgggat tgtcaatgcc ccaacgagat ccgggaatgg
     4081 tgcgaccggc caccgggtgg ccgatccggg tgatgcagct gaccttcggc cgcagcttgt
     4141 cctggttcga cgaccacacc gccgccgaga acatcacctc ggtgaacgcg gcgtcgaacc
     4201 gctcgcgcat cgcgtcggac gccttcgcgc ggctgagcca cgtctcggcg acgctgcgat
     4261 aggccgcctt cacggtcgcg tgctcggtca gttcgagcgc cgcgagttcc tgttcgtgtt
     4321 gcgacgccgt cgccacggga tcgccggtga aatctgacat cacgtcctcc tcgagggaat
     4381 ggcttgggag cgaaagcgcg cgttgtaggc cgcgaagcgt tcgtcgatct gcgcgtcccg
     4441 cagaccgaag tcgaccgcgg cgtaggcggg acgggcaggt tcacgtggcc ggtcgaccag
     4501 ccagcggcgc atcgccgtc
//
LOCUS       DASYLX010000452         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1336510,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000452 DASYLX010000000
VERSION     DASYLX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336510"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VGP27_10990"
     CDS             <1..177
                     /locus_tag="VGP27_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851865.1"
                     /translation="VRGQVSDLVSEDRAQEFLARFPQVEFVDVQGAGHMVAGDRNDLF
                     AQAVVEFLNRHSAH"
     gene            198..1097
                     /locus_tag="VGP27_10995"
     CDS             198..1097
                     /locus_tag="VGP27_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7851866.1"
                     /translation="MRGSKILITGPSGQVAAPIAKSLAADNEVWGIARFSDPAARDGL
                     DKAGVRCETVNLASGDFSGLPADFDYVLNLAVAKSGRWDKDLCANAESAGLLMAHCRT
                     AKAFLHCSSAAVYDPPDDEPRTERAALGDNHKPMFPTYSISKIAGEVVVRTMSRALGV
                     PAIIARLNVPYGDNGGWPFFHMEMMLGGIPIPVPDGGPAVYNPIHEDDIIAMIPTLLE
                     KAAVPAPTINWCGEQAVSLQEWCTYIGSLIGKEPVFEKSVQALRGNPTDPTLMRELVG
                     GTAVDWQDGFRRMVVKFHPELVG"
     gene            1102..>1826
                     /locus_tag="VGP27_11000"
     CDS             1102..>1826
                     /locus_tag="VGP27_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851867.1"
                     /translation="MAERVSFPSTTGPTLAGLVDPPEGEVRGWGVFAHGFTLGKDSPA
                     ASRICKQLASEGVGMLRFDNLGLGDSEGDWGDGSFSHKVADTARAVEFMNDSGREIHL
                     LVGHSFGGSAVLAAAHDCPSVAAVASVGAPYDPGHVEHNYDALLARIEAEGEAPFPVG
                     GKALTLKRHFIEDVRKADLREQIRTLRRALLVLHSPTDNTVGIANASEIFRAARHPRS
                     FVSLEGADHLLTGKNQAARAARI"
BASE COUNT          306 a          649 c          597 g          274 t
ORIGIN      
        1 gtccgcggcc aggtgagcga cctcgtcagc gaggatcgcg cgcaggagtt cctcgcgcgc
       61 ttcccgcagg tcgagttcgt cgacgtgcag ggggcgggac acatggtggc gggcgaccgc
      121 aacgatctct tcgcgcaggc cgtcgtcgag ttcctcaacc ggcactccgc gcattgagca
      181 ttccctacga tcagcgcatg cgcggatcca aaatcctcat caccggaccg agcggccagg
      241 tcgccgcacc catcgccaaa tccctcgcgg cggacaacga ggtatggggc atcgcccgct
      301 tcagcgatcc ggcggcacgc gacggtcttg ataaggccgg ggtgcgatgc gagacggtca
      361 acctcgcgtc gggtgacttc agcgggcttc cggccgactt cgactacgtg ctcaacctgg
      421 cggtcgccaa gagcggcagg tgggacaaag atctctgcgc caacgccgaa tcggccggcc
      481 tgctgatggc gcactgccgc actgcgaagg cgttcctgca ctgctcgtcg gcggcggtct
      541 acgacccgcc cgacgacgaa ccgcgaacgg agcgtgccgc gctcggcgac aaccacaagc
      601 cgatgttccc aacgtattcg atctccaaga tcgccggcga ggtcgtcgtg cggacgatgt
      661 cgcgcgcact cggcgtgccc gccatcatcg ctcgcctcaa cgtgccgtac ggcgacaacg
      721 gtggatggcc gttctttcac atggagatga tgctgggcgg catcccgatc ccggttcccg
      781 acggcggacc cgccgtgtac aacccgatcc acgaagacga catcatcgcg atgatcccga
      841 cgctgctcga gaaggcggcg gtgcccgccc ccaccatcaa ctggtgcggc gaacaagcag
      901 tcagcctgca ggagtggtgc acctacatcg gctcgctgat cggcaaggag cccgtcttcg
      961 agaagagcgt gcaggcgctg cgcggcaatc cgacggaccc gacgctgatg cgtgagctgg
     1021 tcggtggcac cgcggtcgac tggcaggacg gcttccgtcg gatggtcgtc aagttccatc
     1081 cagagctggt cggctaggtc ggtggcggag cgcgtcagct tcccgagcac caccgggccc
     1141 acgctcgcag gtctggtcga cccccccgag ggcgaggtgc gcggttgggg tgtcttcgca
     1201 cacggcttca cgctcggtaa ggacagccct gccgccagca ggatctgcaa gcagctcgcg
     1261 agcgagggcg tcggcatgct gcgcttcgac aacctgggtc tcggcgactc ggaaggggac
     1321 tggggcgacg ggtcgttctc ccacaaggtc gccgacaccg cccgcgccgt cgagttcatg
     1381 aacgactccg gccgcgagat ccacctgctc gtcgggcatt cgttcggcgg gtcggccgtg
     1441 ctcgccgccg cccacgactg cccgtccgtc gcggcggtgg ccagcgtcgg cgcgccgtac
     1501 gaccccggcc acgtcgaaca caactacgac gcgctgctgg cccgcatcga ggccgagggc
     1561 gaggcgccgt tcccggtcgg cgggaaggcg ctcaccctca agcggcactt catcgaagac
     1621 gtccgcaagg ccgacctccg cgaacagatc cgcaccctgc gccgcgccct gctggtcctg
     1681 cattcgccca ccgacaacac cgtcggcatc gccaacgcga gcgagatctt ccgagccgcg
     1741 cgacaccccc gcagtttcgt gtccctggaa ggcgccgacc acctgctcac cggcaaaaac
     1801 caggccgccc gtgcggcccg gatcat
//
LOCUS       DASYLX010000453         9629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1226601,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000453 DASYLX010000000
VERSION     DASYLX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226601"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VGP27_11005"
     CDS             complement(<1..687)
                     /locus_tag="VGP27_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV7851868.1"
                     /translation="MGRLFGTDGVRGVANRELTAELALGLGSAAARRLGNSSQARRRV
                     AVVGRDPRASGEMLEAAVIAGLTSEGVDALRVGVLPTPAVAYLTNAYDAEFGVMISAS
                     HNPMPDNGIKIFGAGGHKLDDATEDRIEELVAAGPGERPVGAAIGRVVDAADALDRYL
                     RHAAKALTTRLDELTVVVDCAHGAASAAAPRAYRAAGANVIAINAEPNGLNINDGCGS
                     THMRTLQQAVV"
     gene            840..1598
                     /locus_tag="VGP27_11010"
     CDS             840..1598
                     /locus_tag="VGP27_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEV7851869.1"
                     /translation="MRSDLFSPQNAETQTQQPGMKLQNGKMLKVALNGEMMVRQGAMV
                     AYQGNIQFQGLGASGVGQFVKQHLTGEGVPLMRCTGAGDMFLADLASDIHLLDLEGPH
                     DGLTINGKNVLAFEPTLSYDIRRVQGAGMLSNAGLFNCVFSGQGRIAVTTKGTPVVLQ
                     VDQPTYADPQAAVCWSASLQTGFHRADQFGLGTLIGRSTGEAFTMSFSGQGFVVVQPS
                     EEMPRSLVSGTGGGQQAGSAGSAAGQILGNFLGR"
     gene            complement(1620..2066)
                     /gene="rpsI"
                     /locus_tag="VGP27_11015"
     CDS             complement(1620..2066)
                     /gene="rpsI"
                     /locus_tag="VGP27_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV7851870.1"
                     /translation="MTETETAAPEARAPREPVIIDRPIQTVGRRKEAVVRVRLVPGTG
                     QFHLDGRTLEAYFPNKVHQQLIKAPLVTVDRVDSFDIYAHLDGGGPSGQAGALRLAIS
                     RALIVVQPEDRPALKKAGFLTRDPRAIERKKYGLKKARKAPQYSKR"
     gene            complement(2141..2584)
                     /gene="rplM"
                     /locus_tag="VGP27_11020"
     CDS             complement(2141..2584)
                     /gene="rplM"
                     /locus_tag="VGP27_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV7851871.1"
                     /translation="MPTYTPKAGDTTRTWYVIDATDVVLGRLAVAAANLLRGKHKPTF
                     TPNVDGGDFVIVINADKVAIGGDKLQKKFTYRHSGFPGGLHRRSLGDEMQRHPDRVVE
                     KAIVGMLPHNKLSRQIQKKLKVYAGAEHPHTAQKPEPYEIKQVSQ"
     gene            complement(2864..3370)
                     /locus_tag="VGP27_11025"
     CDS             complement(2864..3370)
                     /locus_tag="VGP27_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851872.1"
                     /translation="MHKRTHHVAAALLAGLCSPSLTVMGYLTPTAAADTTLSLYEHDT
                     EQHVVDHGSPGPGPGDQFIFAGDVFDRPGGIFLGRANGICTTLTGDDKGGQTTCSVTF
                     NLDGGQVAAQGMLDSAALFVSGNPNPLFITGGTGIYRNARGDGTIQVPIDVPDQTDAN
                     FVLDIVTP"
     gene            complement(3526..3822)
                     /locus_tag="VGP27_11030"
     CDS             complement(3526..3822)
                     /locus_tag="VGP27_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEV7851873.1"
                     /translation="MEPVLSYNFDEIEYTVRQEIHNTSARLNAALDDLRAQITPLQQI
                     WTREAAEAYRLEQAKWDQAAGALNEILYSLGNAVRDGADDVATTDRNAAGAWGG"
     gene            complement(3834..4157)
                     /locus_tag="VGP27_11035"
     CDS             complement(3834..4157)
                     /locus_tag="VGP27_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEV7851874.1"
                     /translation="MTTPAGGSLNTDFELMTAVAGKIDTGNDEIRAMLQSFIGRMVNV
                     PSSVWGGVAATRFRDVVDRWNAESAKLHIALQRIAETMRTNERALREAAESHSHQIGA
                     VGTNI"
     gene            complement(4230..5393)
                     /locus_tag="VGP27_11040"
     CDS             complement(4230..5393)
                     /locus_tag="VGP27_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated protein"
                     /protein_id="HEV7851875.1"
                     /translation="MTECVVEVGPGTVRGSCRAAPNLVSTALDCIDDDIAILDEQPVA
                     VTAVWRDVFRTVLPEPVEAAVLVCPTWWPPPWVDRVAEAAATRSSRADVRHRADVLTA
                     GVPGEPTIVEIAPDFVAVWRAGEVITADPRLGEAADVANSVANSVGPATSVLVDAPVG
                     IAGAVELARAICERLRTTGIAATTVHPDRVLATTRERPREANESKPLRRRRHRFAAPA
                     ASAAAVVLLCAGLSLAPGAEEHDVTAVPMTLLVEGRVALKVPALWAVQRITSGAGSAR
                     VEVRAPDNATAVLVTQSRVPEGETLSATSAMLRRALEGQETGVFSQFKPDGRRADRTA
                     VTYRELRGGRQIDWAVFVDDTVRIGVGCQSAPGGELAVRYACEEAIKSAHALI"
     gene            complement(5390..9136)
                     /gene="eccCa"
                     /locus_tag="VGP27_11045"
     CDS             complement(5390..9136)
                     /gene="eccCa"
                     /locus_tag="VGP27_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HEV7851876.1"
                     /translation="MATEEVAVQAPPDVPRDMPGNPLSRLLPAAMVVATVGMMAFYFT
                     SGASAMRSPMFMFFPVMMLISLVGTAASGSRGGSRIHEINRDRRDYLRYLALLDAKLA
                     KTVEEQRRASWWKHPDPGSLWALVGGRRMWERKPDDTDFGHVRLGLGIRPLSTPLVTP
                     ELGPPDDIDPVTSMELRRLLRDRSMVPDLPVPFALREFAAVTVNGDISAARSLLRAVA
                     CQLAAMHSPQDLRIAVAVDPITARDWDWLKWLPHHQHPYGVDDGGSARLTYGSLSSAE
                     AALSGCRGAHGVLLVDGGLITGAEGLFTGRSADGMTVLELGTTCDDIAGLHLVFIGDD
                     LVAHSDTGDDVVSRPDGMTPGQAMACARRMSRFRPAATTAAGPVCISPSLGWSELMGI
                     ADVGDVDPITLWRATRRPENMLSVPIGVTELGEAVELDIKEAAANGMGPHGLCIGATG
                     SGKSEFLRTLTLGMITAHPPDGLNLVLVDFKGGATFLGLERAPHTSAVITNLADEAHL
                     VARMRDALAGEMTRRQELLRAAGNFANVADYAGARKRNAGLAPLPALFIVVDEFSELL
                     SQQPDFIDLFIAIGRLGRSLGMHLLLASQRIDEGRLRGLESHLSYRVCLKTFSASESR
                     SVLGIADAYNLPRTPGAAYLKTGAADPVRFQTAFVSGPYDSRPSKSAESTATPVPRLF
                     TAAPMGRIAFGTDQRTEGPPASARTVLDTVLDRLEGRGSPAHRVWLPPLATSPDLDVL
                     LQQAGARVALTVPIGLVDRPFEQRRDLFVAQLDGRGGNVAVVGGPRAGKSTALRTMVL
                     ALAETHDPRDVQIYCLDFGGGALSSVRELPHVGSVASRRDVDLIRRTVAQLESLLRAR
                     EAQRSSGDSDTPGDLYGEVFLVIDGWATIRQEFDALEARVTSIAAQGLSYGIHVVVSA
                     SRWAEIRPALKDQLGTRIELRLGDPAESEMDRKRARQLMKSPPGRGITRDGHEFVIAL
                     PRFGAAIADRLRTRYGGRAAPLIQVLPARIGHEAMVARSQDPCSAMRILVGISESELR
                     PVALDFAAESHLIIIGEGECGKTATLRTVCREIVRTNASDAAQLYIVDFRRTLLGVVE
                     SDHLAGYTPSASALTSQLVDLMERLQARVPGADVSRQQLRARSWWSGPEIYVVIDDYD
                     LIAPAAGTNPLAPLVDFLPHAKDLGLHVVVARRSGGAARAMFDPVLARLRDLGCMGLM
                     MSASPDEGVLIGSVRPSQLPPGRATLVTRAHPDRLLQIAWSDPA"
     gene            9282..>9629
                     /locus_tag="VGP27_11050"
     CDS             9282..>9629
                     /locus_tag="VGP27_11050"
                     /inference="COORDINATES: protein motif:HMM:NF020396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEV7851877.1"
                     /translation="MTVVDLCRVTVRTLGPQSVEAVDLTLPTRLELGQLLPDIVDLVV
                     NPPHAGERWSLTRIDGTLLDESMTLQQSGVDDGDVLLLSAETSVLAARYSDMSHYVVD
                     ASGSADRDSGWTRR"
BASE COUNT         1632 a         3209 c         3131 g         1657 t
ORIGIN      
        1 cacgacggcc tgctgcagcg tccgcatgtg ggtggatccg cagccgtcgt tgatgttcag
       61 cccgttgggc tcggcgttga tggcgatgac gttggcgcct gcggccctgt acgcccgtgg
      121 cgcggcggcc gaagcggcgc cgtgggcgca gtcgacgacg acggtcagct cgtccagccg
      181 ggtggtgagg gccttggccg cgtggcgcag gtaccggtca agggcgtcgg cggcgtcgac
      241 gaccctgccg atggcagcac caaccggccg ctcgccaggt ccagcggcga ccagttcctc
      301 gatgcggtcc tcggtggcgt cgtcgagctt gtgcccgccc gcgccgaaga tcttgatgcc
      361 gttgtcgggc atcgggttgt gcgacgccga gatcatcacg ccgaattcgg cgtcgtaggc
      421 gttggtcaga taggcgacgg cgggtgtcgg cagcacaccg acccgcagcg catcgacacc
      481 ctcgctggtg agtccggcga tgaccgcggc ctcgagcatt tcgccgctgg cccgcggatc
      541 gcggcccacc acagcgacgc ggcggcgggc ctgcgaggag tttccaagcc gacgcgcggc
      601 cgccgagccc agcccaagtg ccagctcagc ggtcagctcg cgattggcga ctccgcggac
      661 gccgtcggtg ccgaacagtc gacccatcgg acaaatttct cataaatggc gcggtcggac
      721 acaactgccc acgtcagaag tcgtgccgcc gcggaatcgg tacgcgaccg agatagggaa
      781 acaccctatt tttctggcgg agatcggtcg ataacgtcgc agcgtcgaaa ggatccctta
      841 tgcgcagcga cctgttctcc ccgcagaatg ccgagaccca gacccagcaa ccgggtatga
      901 agctgcagaa cggcaagatg cttaaggttg cgctgaacgg cgagatgatg gtgcggcagg
      961 gcgccatggt ggcctaccag ggcaacatcc aattccaggg gctcggcgcc agcggcgtcg
     1021 ggcaattcgt caagcagcat ctgaccggcg aaggcgtgcc actgatgagg tgcaccggcg
     1081 ccggcgacat gttcctcgcg gacctcgcgt ccgacatcca ccttctggac ctcgagggtc
     1141 cgcacgacgg cctgaccatc aacggcaaga acgtgctggc gttcgagccg acgctgtcct
     1201 acgacatccg ccgcgtgcag ggtgcaggca tgctgtcgaa cgcgggcctg ttcaactgcg
     1261 tgttctcggg tcagggccgc atcgcggtca ccaccaaggg caccccggtg gtcctgcagg
     1321 tcgaccagcc gacctatgcc gacccgcagg ccgcggtgtg ctggtcggcg tcgttgcaga
     1381 cgggtttcca ccgcgcggac cagttcgggc tcggcacgct gatcgggcgc agcaccggcg
     1441 aggccttcac gatgtcgttc tccggtcagg gcttcgtcgt cgtgcaaccg tccgaggaga
     1501 tgccgcgctc gttggtcagc ggcacaggtg gcggccagca ggccggcagc gccggttcgg
     1561 ccgcgggcca gattctcggc aacttcctcg gccgctgacg cacaccgagg tgaagcagat
     1621 cagcgcttgg agtactgagg cgccttgcgg gccttcttga ggccgtactt cttgcgctcg
     1681 atggcccgcg ggtcgcgggt caggaagccg gccttcttca gcgcgggccg atcctcgggc
     1741 tgaaccacga tcagggcacg ggagatcgcc aggcgcaggg cgcctgcctg accggacggg
     1801 ccaccgccat cgaggtgggc gtagatgtcg aagctgtcga cccggtccac ggtcaccagc
     1861 ggagccttga tgagctgctg gtgcaccttg ttcgggaagt acgcctcgag cgtgcggccg
     1921 tccaggtgga attggccggt gcccggcacc agacgcacgc ggaccacggc ctccttgcgg
     1981 cggccgacgg tctggatggg acggtcgatg atcaccggtt cgcggggcgc gcgcgcctcg
     2041 ggggccgcgg tctcggtctc ggtcacggtt tcggtcgcga cggcctcggt gggctcgact
     2101 tccggggttt ccgtggcctc ggtggtgtcg gttgtctcgg tcactggctc acctgcttga
     2161 tctcgtaagg ctccggtttc tgcgcggtgt gcggatgctc cgcccctgca tagaccttca
     2221 gcttcttctg gatctgacgg ctgagcttgt tgtgcgggag catgccgacg atggccttct
     2281 cgaccacgcg gtcgggatgg cgctgcattt cgtcgccgag cgaacggcga tggagaccac
     2341 ccgggaaacc cgagtgacga taggtgaact tcttctgcag tttgtcgcca ccgatggcga
     2401 ccttgtctgc gttgatgacg atgacgaagt cgccaccgtc gacattcggc gtgaatgtcg
     2461 gcttgtgctt gcctcgcagc agattggctg ctgcgacagc gagccggccg agcaccacgt
     2521 cggtggcgtc gatgacgtac cacgtgcgcg tggtgtcacc cgccttcggc gtgtacgtag
     2581 gcacagcgct ttccttatct ctctcggatg attcccggtg acccgggtgc cggtcaggcg
     2641 tttgcgggcg gagtggtctc ggcgaccgac attgacccga gaaccgcgtg cccgaaggcg
     2701 taccgcacgc caaccgagca gcataccggc ggctgtcgcc gcaggtcaaa acgccgtcga
     2761 cggacccatc gagcacccgc tcgccgactt ctgcaccagg gctgtcagcg atcgccggat
     2821 aacgaccctc gtgcagaaat ggacggcagt caaaccgcca cagtcaaggg gtgacgatat
     2881 cgagcacgaa attcgcatcg gtctggtcgg gaacatcgat gggaacctgg atggttccgt
     2941 cgcccctggc attgcggtag atcccggtgc cgcccgtgat gaacaaggga ttcgggttgc
     3001 cgctcacgaa cagggcggcg ctgtcaagca tgccctgagc cgcaacctga ccgccatcga
     3061 gattgaacgt cacgctgcaa gtcgtctggc cgcccttgtc gtccccggtg agcgttgtac
     3121 aaattccgtt ggcccgcccg aggaagatgc cgccgggacg atcgaatacg tcgccggcga
     3181 agatgaactg gtcgccagga ccgggtccgg gcgatccgtg atcgaccacg tgctgctcgg
     3241 tgtcgtgttc ataaaggctg agcgtggtgt ccgccgccgc cgtcggggtc aaatacccca
     3301 ttacggtcag cgacgggctg caaaggccgg cgagcagcgc cgctgctaca tggtgcgtgc
     3361 gcttgtgcat ccgctggccc cccggccgtg tgagttagct ggaggctaca gcccagcgag
     3421 caccaggcgc tcaggtatgg cgacgccgat gccggtccat tggtatgact gtgcggtccc
     3481 gcgggctccc ctcccgacag gaccgcacag tggtcagtga ctgcctcagc ctccccaggc
     3541 gcccgccgca ttgcggtcgg tcgtcgctac gtcgtccgcg ccgtcgcgca ccgcattgcc
     3601 caggctgtac agaatctcgt tcagcgcacc cgccgcctgg tcccatttgg cttgctcgag
     3661 ccgatacgcc tccgccgctt cgcgggtcca aatctgctgc agcggtgtga tctgcgcccg
     3721 caggtcatcg agcgccgcgt ttagtcgcgc cgaagtgttg tggatctcct gtcgaacggt
     3781 gtactcgatc tcgtcgaagt tgtacgagag aaccggttcc atgctcaacc tcctcaaatg
     3841 ttggtgccga cggcgccgat ctggtgtgaa tgactctcgg ccgcctcgcg cagggctcgt
     3901 tcgttggtcc gcatggtttc cgcgatcctt tgcagggcga tgtggagctt ggccgattcg
     3961 gcgttccagc ggtctacgac atccctgaat cgcgttgcgg cgacgcctcc ccagaccgac
     4021 gacggcacgt tgaccatccg tccgatgaat gactgcaaca tggcccggat ctcgtcgttg
     4081 ccggtgtcga tcttgccggc gacggctgtc atcaactcga agtcggtgtt gagcgagccg
     4141 cctgcgggtg tcgtcatcaa agtcctttcc gtgcttttcg atcgcgatgt gatttcgacg
     4201 gaccccgagc gcagttggtt ccccccgact cagatcagtg catgtgcaga cttgatggct
     4261 tcctcgcagg cgtaacggac ggcgagttcg ccgcccggcg cgctctggca gccgacgccg
     4321 atccgcacgg tgtcgtcgac gaacaccgcc cagtcgatct gccgtcctcc acggagttcc
     4381 cggtacgtga cagcagttcg atccgcgcga cgaccgtcgg gcttgaactg actgaataca
     4441 cccgtctcct ggccctcgag cgccctccgc aacatcgctg aggtcgcaga cagcgtctcg
     4501 ccttcgggca cccgcgactg ggtcaccagg acggccgtcg cgttgtccgg cgctctcact
     4561 tccacccgcg ccgagcccgc tcccgacgta atgcgctgca ccgcccacaa cgccggaacc
     4621 ttcagcgcga ctcgtccttc caccagaagt gtcatcggca cggcggtcac atcgtgttct
     4681 tccgcgcctg gcgcaagact caagccggcg cagagcagaa cgaccgctgc cgcggaagcg
     4741 gcgggcgctg cgaacctgtg tcttctccgt cgaagcggct tcgattcgtt cgcttcccgc
     4801 ggccgttccc gcgtggtagc cagtacccgg tcggggtgca ccgtcgtcgc cgcgatgcct
     4861 gtggtccgaa ggcgctcgca gatcgcacgg gccagctcga ctgcccctgc gataccgacc
     4921 ggtgcgtcca ccagcaccga ggtcgccggg ccgacgctgt tcgccactga gttggcgacg
     4981 tccgcagcct cgccgagccg cgggtcggcc gtgatcacct cccctgcccg ccagacagcg
     5041 acgaagtccg gtgcgatctc gacgatcgtc ggctcgcccg gtacgccggc ggtgaggacg
     5101 tcggcacgat gtcgcacatc ggctctcgac gatcgcgtgg ccgcagcctc cgccacgcga
     5161 tcgacccacg gcggcggcca ccacgtcgga cagaccagca cggcggcctc gacgggctca
     5221 ggcaaaacag ttcggaaaac atcacgccat acagcggtga cggcaacggg ctgttcatcg
     5281 aggatggcga tgtcatcgtc gatgcagtcg agcgctgttg acaccagatt cggcgccgcg
     5341 cggcacgatc cgcggaccgt tcccggcccc acctcgacga cacactccgt cacgccggat
     5401 cgctccacgc gatctggagg agcctgtcgg ggtgcgcacg ggtgacgagc gttgcgcgac
     5461 ctggcggcag ctgcgacggc cggacggaac cgatcagcac gccctcgtcc gggctggcac
     5521 tcatcatcag gcccatgcat ccaaggtccc gcagccgagc cagaaccgga tcgaacatcg
     5581 cccgcgccgc accgccggag cgcctcgcca ccacgacatg caaaccgaga tccttggcgt
     5641 gcggcagaaa gtcgaccagc ggcgccagcg ggttggtgcc ggccgcaggg gcgatcaggt
     5701 cgtagtcgtc gatgacaacg tagatttccg gacccgacca ccaggaccgt gctcgcaact
     5761 gctgccggct gacgtccgca ccgggcacgc gcgcctgtag acgttccatc aggtcgacca
     5821 actgcgacgt cagcgccgac gcggacggtg tgtatcccgc gagatggtcg gactcgacaa
     5881 caccgagcag tgtccgacga aaatcgacga tgtacagttg agcggcgtct gacgcgttcg
     5941 tcctcacgat ctcgcggcag accgtccgca gggtggccgt cttgccgcac tccccctcgc
     6001 cgatgatgat cagatgtgac tcagcagcga aatcaagtgc gactggtcgc aattcggact
     6061 cactgatacc caccaggatc cgcatggcgg aacacgggtc ttgcgatcgc gcgaccattg
     6121 cctcgtggcc gatgcgcgcc ggcagaacct ggatgagtgg tgcagcgcgc cctccgtatc
     6181 gcgtcctcaa cctgtcagcg atggcagccc cgaaccgagg cagcgcgatg acgaactcgt
     6241 ggccgtcgcg ggtgatgccg cgaccgggcg ggctcttcat caactgccgg gcccgcttcc
     6301 ggtccatctc ggactccgcc gggtcgccca gccgcagttc gatgcgggta ccgagctgat
     6361 ccttcagtgc gggccggatc tcggcccatc gcgacgccga gacgaccaca tggatgccgt
     6421 atgaaagccc ctgcgctgca atcgaagtga cccgtgcctc caaagcgtcg aactcctgcc
     6481 gaatggtggc ccagccgtcg atgacgagga acacctcgcc gtacaagtcg cccggtgtgt
     6541 ccgaatcgcc ggaactgcgc tgcgcctctc tcgctcgcag cagcgattcg agttgggcga
     6601 cagttcggcg aatcaggtcg acgtcgcgcc tgcttgccac cgaaccgacg tgcggcagtt
     6661 ctcgcaccga ggacagcgca ccgccgccga agtcgagaca gtaaatctgc acatcacggg
     6721 ggtcgtgagt ctcggccagc gccagcacca tcgtccgcag cgccgtggac ttgccggccc
     6781 gcggcccgcc cacgacggcg acatttccgc cccggccgtc gagttgcgcg acaaacagat
     6841 cccggcgctg ctcgaagggg cgatcgacga ggccgatcgg caccgtcagt gcgacacgcg
     6901 ccccggcctg ctgcagcagg acatcgagat cgggcgatgt cgccagcggc ggtagccata
     6961 ctcggtgcgc cggcgatccg cggccctcga gtcgatcgag gaccgtgtcg agaaccgtgc
     7021 gggcggaagc gggcggacct tcggtccgct gatcggttcc gaacgcaatg cggcccatcg
     7081 gcgccgcggt gaacaaccgt ggcaccggcg tggctgtcga ctcagcggac ttcgatggcc
     7141 tgctgtcgta gggtccagag acgaacgccg tctggaagcg cacggggtca gccgcgccgg
     7201 tcttcaagta cgccgcacct ggtgttctgg gcaagttgta ggcgtcggcg atcccgagaa
     7261 ccgaccgaga ttcactcgca gagaaggtct tcagacatac ccgataggac aaatgggatt
     7321 ccaacccgcg cagcctgccc tcgtcgatgc gctgactcgc gagcaggagg tgcatgccca
     7381 gcgaacggcc gagcctgcct atcgcgatga acaagtcgat gaaatccggc tgctggctga
     7441 gtagctcgga gaactcgtcg acgacgatga acagcgccgg taatggcgcg agcccagcat
     7501 tccgcttgcg tgccccggcg tagtcggcca cattcgcgaa gttgcccgcc gccctcaata
     7561 gctcttggcg cctggtcatt tcaccggcca acgcgtctct catgcgcgcc acaaggtgcg
     7621 cttcgtccgc caggttggtg atcaccgccg atgtatgggg tgcgcgctcc aacccgagaa
     7681 aggtcgcacc tcccttgaag tcgacgagga ccaggttcag cccgtcgggc ggatgcgcgg
     7741 tgatcatgcc cagcgtcaga gtgcgcagaa actccgactt gccggagccc gttgcaccga
     7801 tgcacaagcc gtgcggcccc atgccattcg ctgcggcctc cttgatgtcg agctccaccg
     7861 cctcgccgag ctcggttacg ccgatcggga cactcagcat gttttccggc cgacgggtgg
     7921 cacgccagag cgttatggga tcgacgtcgc cgacatcggc gatgcccatc aactccgacc
     7981 atccgagtga tggtgaaatg cacaccgggc cggcggccgt cgtggcagcg ggccggaacc
     8041 gagacattcg gcgggcgcat gccattgcct gtcctggcgt cattccgtcg gggcggctaa
     8101 cgacgtcgtc accggtgtca ctgtgtgcga ccagatcatc gccgatgaag acgaggtgca
     8161 ggccggcgat gtcgtcacac gtcgtgccca gttcgagaac cgtcatcccg tctgcactcc
     8221 gcccagtgaa gaggccctct gcaccggtaa tcaggccgcc gtcgaccagc agcacgccat
     8281 gagctccgcg gcagcccgac agtgcggcct cagcagatga gagactgccg tacgtcaatc
     8341 gcgccgaccc gccgtcgtcg acgccatagg gatgctgatg gtgcggcagc catttcaacc
     8401 aatcccagtc ccttgcggtg atgggatcga cggcgacggc gatgcgtaga tcctgtgggc
     8461 tgtgcatcgc cgccaactga catgccaccg cacgaagcag actcctggcg gcggatatgt
     8521 cgccgttgac ggtgacggcg gcgaactccc tcaacgcgaa cggaacgggt aaatcgggca
     8581 ccatcgagcg gtcgcgcagc aggcgccgca actccatcga tgtgacggga tcgatgtcat
     8641 ccggtggtcc gagctctggg gttaccaaag gcgtcgacaa gggtcgaatg cccagtccga
     8701 ggcggacgtg cccgaaatcg gtgtcgtcgg gcttgcgctc ccacattcgc ctaccgccga
     8761 ccaatgccca gagcgatccc gggtcggggt gcttccacca cgacgcacga cgttgctcct
     8821 caacggtctt cgcgagcttc gcgtcaagaa gcgcaaggta acgcagatag tcgcggcggt
     8881 ctctgttgat ctcgtggatt cgactgcctc cgcgcgaccc agatgccgcc gtgccgacga
     8941 gggagatgag catcatcacc ggaaagaaca tgaacatcgg gctgcgcatc gccgaagcgc
     9001 ccgaagtgaa gtagaacgcc atcatcccta ccgtggcaac caccatcgcc gcggggagca
     9061 acctggacag gggattaccc ggcatatcgc gcggtacgtc cggcggggcc tgcacggcga
     9121 cttcttcggt cgccatgggt ggcgtcgcga tgcgcgaccc gatcaagaac tccaccgagc
     9181 aatagacgag gcggcgcccc gtctcgttcc ccttccacag cagagaaatt gccgtcacag
     9241 gccgcctgcg gacgcggtta ccgtgaccgg cggggggtca catgacggtt gttgatctgt
     9301 gtcgagtaac ggtgcgaacg cttggaccgc agagtgtaga ggcggtggat ctcactctgc
     9361 ccactcggct cgaactcggc caattgctac cggacatcgt cgacctcgtc gtcaatccgc
     9421 ctcatgccgg tgagcgatgg agcctgacac ggatcgacgg aacgctgctc gatgagtcga
     9481 tgacgttaca gcagagcggt gttgacgacg gtgatgtgtt gctgctctcc gctgagacgt
     9541 cagtgctcgc tgcccgctac agcgatatga gccactacgt cgtcgatgct tcgggttcgg
     9601 ccgatcgcga tagcggctgg acccgacgt
//
LOCUS       DASYLX010000454         7315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_349270,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000454 DASYLX010000000
VERSION     DASYLX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349270"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGP27_11055"
     CDS             <1..270
                     /locus_tag="VGP27_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7851878.1"
                     /translation="NTSRGPIIDEDALVDALRGGRLAAAGLDVFAVEPVPADNPLLAL
                     DNVVLTPHVTWYTVDTMRRYLAEAVDNCRRIRDGRDLRNVVNSPE"
     gene            312..2510
                     /locus_tag="VGP27_11060"
     CDS             312..2510
                     /locus_tag="VGP27_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7851879.1"
                     /translation="MPIAVNSEHNDLADSVRSFVARVAPSDVLHEALETPIPNPPPYW
                     KAAAEQGLHGVHLSEAVGGQGFGILELAIVLAEFGYGAVPGPFVPSAIASALISAHDP
                     EAPLLNDLASGAVIAAYAIDSGLTATRQGDGLVIRGEVRAVPAAAQAAVLVLPVAGLD
                     GGTSGYEWVVLDAGQLEIEPVRSVDPLRPLAHVRANAVEVGADRVLSNLSRELARALI
                     STLLSAEAVGVSRWATDTASAYAKIREQFGRPIGQFQAIKHKCAEMIADTERATAAVW
                     DAARAIDESDSGESAFEFAAAVAATLAPVAAQRCTQDCIQVHGGIGFTWEHDCNVYYR
                     RALVLAACFGRAADYPQQVVDIATTTGMRRLDIDLDPDTEKLREEIRAEVAALKALPG
                     GAERNAAIAEGGWVQPHLPKPWGRASDPIEQIIIAQEFSSGMVKRPQMGIAAWIIPSI
                     VAFGTDEQQQRFLPPTFRGEMIWCQLFSEPGAGSDLASLSTKATKVDGGWRITGQKIW
                     TTAAQFSQWGALLARTDPSAPKHNGITYFLLDMNSEGIEVKPLRELTGNTMFNTVFID
                     DVFVPDEYVLGDPDRGWEVSRNTLTAERVSIGNTEPPGMASLAGLVDFVRDGQFDQIA
                     QNQAGRLIAEGHAAKILNMRSTLLTLAGGDPMPAAAISKLLSMRTGQGYAEFAVSSFG
                     TDGAVGDLGSLPGTWAEHLLYSRATTIYGGTSEVQLNIIAERLLGLPRDP"
     gene            2520..3458
                     /locus_tag="VGP27_11065"
     CDS             2520..3458
                     /locus_tag="VGP27_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7851880.1"
                     /translation="MGRTTTDVTLRQLRYFAVLGDELNYRRAAERLFITQPALSTAIK
                     QLEHQLGVMLFTRNTREVALTELGAEWLPKVCDALHGIDSAVDDLIALSAHYRRIRLG
                     YLIGTGADLLFRLIRHFEVGHPEIKLEPREFDFSDPTAGLADGTTEVAIIRPPVDLPE
                     HRMFILDAESWVACLPRDHRLARRSELTIAELLEEPIVVAPSTAGCWRDYWMAMDARD
                     GKPPTIAAVAATYEEETTTIARGLGISFTSEASARLYERQGIVYVPITDRPRNYTALA
                     WHPAGLSHEAAELVRHVREEWSFGEGQPVERPATYY"
     gene            3567..5066
                     /locus_tag="VGP27_11070"
     CDS             3567..5066
                     /locus_tag="VGP27_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7851881.1"
                     /translation="MTDIAPAEAKAPEPVTSEDAKLEQFGYTQKLDRSVGRLASFALG
                     FATISATTAVFTGFGAGYFTAGGPFVWTLLLAAAVFLIWTFIAADLCAKIPLAGYAYQ
                     WTSRIHGSVMAFFTGVLALAGWVCGMTGVGFILSGYLGSLFGWNMSQSAQILVAIGVM
                     AVCMLVNLYGVRFATMVNNIGVSLELVVTVGATLLVAIVALSSPANHQPISVLFTGGT
                     SDQHSAYVLAWLAASLGPFFGLIGVEASADIAEETVNARRVIPRTMFYALTASIVIEF
                     LMYVVYVLAIKDAGAVEAASAAPIEEIITQQVGPVVAQIVVAVALTNVMACILANILV
                     ATRLTYSMARDNMLPFSHVWRHVSPSNRTPTYAVLGLFGLSTILLLSALISEKAFFLI
                     IGLSSLAVCAMYFLQTVAVLVASRRGTIPAPEPGTFDLGRARVPVAIVALIAFFLVCA
                     ALIFLPQFANNGYVFGGLVVLAGIWAMTGLRKRLANGDAGPDYSKTHLS"
     gene            5098..6114
                     /locus_tag="VGP27_11075"
     CDS             5098..6114
                     /locus_tag="VGP27_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV7851882.1"
                     /translation="MTTLDIGASNLVAVEPGAIREDTPPGSVIQYSDYTLDASSPFAG
                     GVAWIEGEYVPAEEARISIFDTGFGHSDLTYTVAHVWHGNIFRLGDHLDRLLDGARKL
                     RLDAGYPKDELAEITKRCVSMSQLRESFVNLTVTRGYGKRKGEKDLSKLTHQVYIYAI
                     PYLWAFPPSEQIFGTTAIVPRHVRRAGRNTVDPTIKNYQWGDLTAASFEAKDRGARTA
                     ILMDSDNCVAEGPGFNVCIVKEGKLASPSRNALPGITRKTVFEIAETMGIEASLRDVT
                     SHELYDADELMAVTTAGGVTPINSLDGEPIGSGEPGPMTVKIRDRFWALMDEPSPLIE
                     AIEY"
     gene            6234..6512
                     /locus_tag="VGP27_11080"
     CDS             6234..6512
                     /locus_tag="VGP27_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851883.1"
                     /translation="MKIIRQRIVRLIVSAAAAAAFLIATPALFSETVPQASAQPCVNG
                     VIPWNPYVVNCNLPPRGPRVRGGAPDAGAIIACRNHPGCLSWYVNGPW"
     gene            6591..6875
                     /locus_tag="VGP27_11085"
     CDS             6591..6875
                     /locus_tag="VGP27_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851884.1"
                     /translation="MFRQAVTYGVGVIVVAGIAFVVFAVVDKGSVVLASTVPAILFLG
                     GVGAFVKAYRVWRAGGTWPIWQGAGWFLLTLMLLCLSIPWSAAIFGKSSH"
     gene            complement(6938..>7315)
                     /locus_tag="VGP27_11090"
     CDS             complement(6938..>7315)
                     /locus_tag="VGP27_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557788.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7851885.1"
                     /translation="VSGRDDEMIVSGGENVFPAEVEDLISGHPDVVEATALGVEDKDW
                     GHRLRAFVVKTDGASIDEDTIKTYVKENLARYKVPREVVFLEELPRNPTGKILKRELR
                     EMAVGGDDEKAEVGGDDEKAEVE"
BASE COUNT         1222 a         2476 c         2350 g         1267 t
ORIGIN      
        1 aacacctctc gcggacccat catcgacgaa gacgctctcg tcgacgcgtt gcgaggaggt
       61 cggctggcgg cggcgggact cgacgtcttc gccgtcgaac cggtacccgc cgacaacccg
      121 ttgttggccc tcgacaacgt cgtgctgaca ccgcacgtca cctggtacac cgtcgacacc
      181 atgcgcagat acctggccga agcggtcgac aactgccgcc ggatccgcga cgggcgggac
      241 cttcgaaacg tcgtcaactc acccgagtag tctcgtccca accgaccggt tattagggaa
      301 ggcacttcgg catgcccatc gcagtcaatt ctgagcacaa cgacctggcc gattcggttc
      361 gatccttcgt ggcgcgggtg gcgccatcgg acgttctgca cgaggccctc gaaacgccga
      421 tcccgaaccc gccgccctac tggaaggctg ccgccgaaca aggcctgcac ggcgtccacc
      481 tgtccgaagc cgttggcgga caagggttcg gaatcctgga actcgcgatc gtgctcgccg
      541 agttcggcta tggagcggta cccgggccct ttgtgccgtc cgccatcgcc agcgcgctga
      601 tctcggcaca cgatcccgag gccccgctgc tcaacgacct ggcatccggc gcagtgatcg
      661 ctgcgtacgc catcgactcc gggctcaccg ccacccggca gggcgacggc ctggtcattc
      721 gcggcgaggt acgggcagtg cccgcggcag cgcaggccgc ggtgctggtg ctgcctgtgg
      781 ccggcctgga tggggggacc agcggctacg aatgggtggt gctcgacgcc ggtcaactgg
      841 agatcgaacc cgtccgaagt gtcgacccgc tgcgaccgct cgcgcatgtc cgcgcgaacg
      901 ccgtggaggt cggcgcggac cgggtgttga gcaacctgag ccgcgaactg gcccgcgcgc
      961 tgatatcgac gctgctgtcg gccgaagcgg tcggcgtctc gcggtgggcc acggacaccg
     1021 catcggccta cgccaagatc cgcgagcagt tcggcaggcc gatcgggcag ttccaggcca
     1081 tcaagcacaa gtgcgcggag atgatcgccg acaccgaacg cgcgacggcc gcggtctggg
     1141 atgctgcccg cgcgatcgac gaaagcgatt cgggcgaaag cgctttcgag ttcgcggccg
     1201 cggtggcggc aactctggcc cctgtcgcgg cgcagcgctg cacccaggac tgcattcagg
     1261 tgcacggcgg catcggcttc acatgggaac acgactgcaa cgtgtactac cgccgcgccc
     1321 ttgtgctggc ggcctgcttc ggccgcgccg ccgactaccc gcaacaggtc gtcgacatcg
     1381 ccaccaccac cgggatgcgc aggctcgaca tcgatctgga cccggacacc gagaagctgc
     1441 gcgaggagat tcgtgcggag gtggccgcgc tcaaggcact tcccggcggc gccgagcgca
     1501 acgccgcgat cgcagaaggt ggctgggtgc agccgcatct gccgaagccg tggggtcgcg
     1561 cctccgaccc gatcgagcag atcatcatcg cccaggagtt ctcgagcggc atggtgaagc
     1621 gcccgcagat gggcatcgcg gcgtggatca ttccgtcgat cgtcgcgttc ggcaccgacg
     1681 agcagcagca gagatttcta ccgccgacgt tccgcggcga aatgatctgg tgtcagctct
     1741 tttcggagcc tggcgccggc tccgacctgg ccagcctgtc caccaaggcc accaaggtcg
     1801 acggtggctg gcgcatcacc ggtcagaaga tctggaccac cgcggcgcag ttctcgcaat
     1861 ggggtgcgct gctcgcacgg acggacccca gcgcacccaa gcacaacggg atcacctact
     1921 tcctgctcga catgaacagc gaaggcatcg aggtgaagcc gttgcgcgag ctcaccggca
     1981 acaccatgtt caacaccgtc ttcatcgacg acgtgttcgt tcccgacgag tacgtgctcg
     2041 gcgacccgga tcgcggctgg gaggtcagtc gcaacacgct taccgcagag cgggtctcga
     2101 tcggcaacac cgagccgccg ggcatggcga gcctggctgg tctggtcgat ttcgtccgcg
     2161 acggccagtt cgaccagatc gcgcagaacc aggccggccg gctgatcgcc gaggggcacg
     2221 ccgccaagat cctgaacatg cgctcgaccc tgctgacgct ggcaggtggc gatccgatgc
     2281 ccgccgccgc gatcagcaag ctgctgtcca tgcggacggg ccagggctac gccgagttcg
     2341 ccgtgtcctc cttcggcacc gacggcgccg tcggcgatct cggatcattg ccgggcacgt
     2401 gggccgagca cctgttgtac agccgcgcca ccacgattta cggtggcacg tcggaggtgc
     2461 agctcaacat catcgccgag cggctgttgg ggctgccccg cgacccctag aggtaggagg
     2521 tggggcggac gaccaccgac gtgacgctgc gccaactgcg gtacttcgcc gtgctcggcg
     2581 acgagctcaa ctatcgccgc gccgccgagc ggctgttcat cacccaaccc gcgctgtcga
     2641 cggcgataaa gcagctcgag caccaactcg gggtcatgct cttcacccgc aacacgcgcg
     2701 aggtggcgct gacggaactc ggcgccgaat ggctgcccaa ggtgtgcgat gcgctgcacg
     2761 gaatcgactc cgcggtcgac gatctgatcg cgctgtcggc gcactaccgg cgcatccggc
     2821 tgggctacct catcggaacc ggggcggatc tgctgttccg cctgatccgc catttcgagg
     2881 ttgggcatcc cgaaatcaaa ctggagccac gggaattcga cttctcggat cccactgcgg
     2941 gacttgccga cggaaccacg gaggtggcga tcatccggcc gcccgtcgac ctgcccgagc
     3001 accggatgtt catcctggac gccgagagct gggtcgcctg cctgccgcgg gaccatcggc
     3061 tggcccggcg atcggagttg accatcgccg aattgctcga ggagcccatc gtggtcgcgc
     3121 cgagcaccgc cgggtgctgg cgtgactact ggatggcgat ggatgcccgc gacggcaagc
     3181 cgccgacgat cgccgccgtc gccgccacct acgaagagga gaccaccacc attgcgcgcg
     3241 gcctcggcat cagcttcacc agcgaggcaa gcgcccgcct gtacgagcgg cagggcatcg
     3301 tctacgtgcc gatcaccgac cggccgcgca attacaccgc gctcgcgtgg caccccgccg
     3361 ggctgtcgca tgaagccgcc gaactcgtcc ggcacgtgcg ggaggaatgg agcttcggcg
     3421 agggccagcc ggtcgaacgg cccgcgactt attactgaca ccactgataa gcgttggtta
     3481 tcagtagatc agttattgga acttccgccg agcaggtcaa aggggttttc tgtgaacacc
     3541 gctcgacccg tctgcctgga gaagccatga ccgatatcgc acccgccgag gcgaaagctc
     3601 cggaacccgt caccagcgag gatgccaagc tcgagcagtt cggctacacc cagaagctcg
     3661 accgctcggt gggccgactc gcgtccttcg cgctcggctt cgcgacgatc agcgctacca
     3721 ccgcggtctt caccggcttc ggcgcgggtt acttcaccgc tggcggcccg ttcgtgtgga
     3781 ccctgctgct cgccgcagct gtgttcctga tctggacgtt catcgccgcc gacctgtgcg
     3841 cgaagatccc gctggccggc tacgcctacc agtggaccag ccgcatccac ggctcggtga
     3901 tggccttctt caccggcgtg ctggcactcg caggatgggt ctgcggcatg acaggcgtcg
     3961 ggttcatcct gtccggctat ctcggcagct tgttcggctg gaacatgagc cagagcgcgc
     4021 agatcctcgt cgccatcggt gtgatggccg tgtgcatgtt ggtgaatctc tacggggtgc
     4081 ggttcgcgac gatggtcaac aacatcggcg tcagcctcga actcgtcgtc accgtcggcg
     4141 caacgctgtt ggtcgccatc gtcgcgttgt cctcacccgc gaaccaccag ccgatctcgg
     4201 tgctgttcac cggcggcacg tcggaccagc acagcgccta cgtcctggcc tggttggccg
     4261 cctccctcgg gcccttcttc gggctgatcg gcgtcgaggc cagcgcggac atcgccgagg
     4321 aaaccgtcaa tgcccgaagg gtcattccca ggacgatgtt ctacgcgctg acggcatcga
     4381 tcgtcatcga gttcctgatg tatgtggtct acgtgctggc gatcaaggat gcaggcgcgg
     4441 tcgaggccgc ctcggcggcg cccatcgagg agatcatcac gcagcaggtc ggcccggtcg
     4501 tcgcacagat cgtcgtcgcc gtggcgttga ccaacgtgat ggcgtgcatc ctcgccaaca
     4561 tcctggtggc cacccggctc acctactcga tggcccgcga caacatgctg cccttctccc
     4621 acgtatggcg gcacgtgtcc ccgtccaacc ggacgcccac ctacgcagtg ctcggcctgt
     4681 tcggtctgtc gaccattctg ctgctgtcgg ccttgatcag cgaaaaggcg ttcttcctca
     4741 tcatcggtct gtcatcgctg gcggtgtgcg cgatgtactt cctgcagacg gtggccgtgc
     4801 tggtcgccag ccgccgcggc accatccctg cgccggagcc cgggacgttc gacctgggcc
     4861 gggcccgcgt gccggtcgcc atcgtcgccc tcatcgcgtt cttcctggtc tgcgccgcac
     4921 tgatcttcct gccgcaattc gccaacaacg gctacgtgtt cggcggcctc gtggtgctcg
     4981 caggcatctg ggcaatgacc gggctacgca agcggctcgc caacggtgac gccggaccgg
     5041 actactccaa gacccacctg agctgaattc gcctctctca ctgaaggatt acgcaaaatg
     5101 acaacccttg atatcggcgc aagcaacttg gtcgccgtgg agcccggcgc gatccgggag
     5161 gacaccccgc ccggctcggt gattcagtac agcgactaca cgctggacgc atcgagcccg
     5221 ttcgcgggcg gggtggcctg gatcgaaggc gagtacgtgc ccgcagaaga agcccgcatc
     5281 tcgatcttcg acaccggctt cgggcactca gacctgacct acaccgtcgc acatgtatgg
     5341 cacggcaaca tcttccggct cggcgatcac ctcgaccggc tgctcgacgg cgcacgcaag
     5401 ttgcggctcg acgccggcta cccgaaggac gagctggccg agatcaccaa gcggtgcgtc
     5461 agcatgtcgc agctgcggga gtcgttcgtc aacctgactg tcacgcgggg ttacggaaaa
     5521 cgcaagggcg agaaggatct ttccaagctc acccaccagg tctacatcta tgcgatcccc
     5581 tacctgtggg cgttcccgcc aagcgagcag atcttcggga cgacggcgat cgtcccacgg
     5641 catgtgcgcc gcgccggtcg aaacaccgtc gacccgacca tcaagaacta ccagtggggc
     5701 gacctgaccg cggccagctt cgaggcgaag gaccgcggcg cgcgcactgc gatcctgatg
     5761 gactccgaca actgcgtcgc cgaggggccg ggcttcaacg tctgcatcgt caaggagggg
     5821 aagctggcct cgccgtcccg aaatgcgctg ccgggcatca ccagaaagac ggtgttcgag
     5881 atcgccgaga cgatgggcat cgaggccagc ctgcgcgacg tcaccagcca cgagctctac
     5941 gacgccgacg agttgatggc ggtcaccacc gcgggcggcg tcaccccgat taactcgctc
     6001 gacggtgagc cgatcggcag cggcgaaccc ggaccgatga cggtgaagat ccgcgaccgg
     6061 ttctgggcgt tgatggacga gccctcgccg ttgatcgagg cgatcgagta ctgacgaacg
     6121 cccgcacagc ggggccgtcg atggcagttc cgcaacgcca ttccgccgct gacggtgtag
     6181 cgggggtcta tcctcgaaat agatagcttc ccaaagctgt tggcggtggt gagatgaaaa
     6241 ttatccgaca acgcatagtg cgcctgatag tcagtgcagc ggccgcggcc gcgttcttga
     6301 tcgcgacccc cgcgctgttc agcgagacag ttccgcaagc gtcggcccag ccgtgtgtaa
     6361 acggcgtcat cccgtggaac ccgtacgtcg tgaattgcaa ccttccgccc cgcggaccca
     6421 gggtgcgcgg cggcgctccc gacgcggggg cgatcatcgc gtgccggaac catcccggct
     6481 gcctgtcctg gtacgtcaac gggccctggt agtttccgcg gcaccgccgg gggaacccga
     6541 tccgcatgag cgatgcaaga gatgttgaga agcgctggca cgaccctgcc atgttcaggc
     6601 aggccgttac gtacggcgtc ggcgtcatcg tcgtggccgg catcgcgttc gtggtgttcg
     6661 ccgtcgtcga caaggggtcc gtcgtcctgg cctctaccgt gccggcgatc ttgttcctcg
     6721 ggggcgtcgg cgcgttcgtg aaggcgtacc gcgtctggcg cgccggcggc acatggccga
     6781 tctggcaggg cgcgggttgg ttcttgctga ctctgatgct gttgtgcctg tcgataccgt
     6841 ggtcagcggc aattttcggc aagtcttcgc attgagtcgg tccgccgccc gagcggtacc
     6901 tgaattggcg gactattggc tataggcgcg aaacccgtta ctccacttct gccttctcgt
     6961 cgtctccgcc gacttctgcc ttctcgtcgt ctccgccgac ggccatttcg cggagttcgc
     7021 gcttgagaat cttgccggtc gggttgcgcg gcagttcctc gaggaagacg acttcgcgcg
     7081 gcaccttgta gcgggccaag ttctccttga cgtaggtctt gatcgtgtcc tcgtcgatgc
     7141 tggcgccgtc ggtcttgacg acgaacgcgc gcagccggtg gccccagtcc ttgtcctcga
     7201 cgccgagcgc ggtggcctcg acgacgtcgg ggtgaccgct gatgaggtcc tcgacttctg
     7261 ctgggaagac gttctcgccg ccggacacga tcatctcgtc gtcgcggccg ctgac
//
LOCUS       DASYLX010000455         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2434236,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000455 DASYLX010000000
VERSION     DASYLX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434236"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="steA"
                     /locus_tag="VGP27_11095"
     CDS             complement(<1..631)
                     /gene="steA"
                     /locus_tag="VGP27_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEV7851886.1"
                     /translation="MKMSALLSRTASPRPGITGTARVDRDIDRLLRRVGPGDIVVIDA
                     LDLDRITADALVEAEIAAVVNASPSISGRYPNLGPEVLVNNGVTLIDETGPEIFKRIK
                     DGARVRLYDGGVYAGDRRLAHGTERTDHEIHELMHEAKSGLVAHLEAFAGNTIEFIRS
                     ESPLLIDGIGIPDIDVDLHRRHVVIVAEEPDAAYDLKALKPFIKEYQPVL"
     gene            complement(723..2489)
                     /gene="recN"
                     /locus_tag="VGP27_11100"
     CDS             complement(723..2489)
                     /gene="recN"
                     /locus_tag="VGP27_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV7851887.1"
                     /translation="MLSEIRIESLGAISTATAEFDQGLTVLTGETGTGKTMVVTGLHL
                     LGGARADATRIRSGADRAVVEGRFSTVELGDGVAAQIDKILDASGADRDEDGSVIAAR
                     SVSRAGPSRAYLGGRSVPAKSLSTFTTELLTLHGQNDQLRLMRGDEQRAALDRFADVG
                     ALLQRYRTVRESWLAARRDLAERTARAREMAQEADRLTFGLGEIDAVDPRPGEDDALV
                     DDIRRLSELDALREAARYARTALAGALDDTADADAASASHEVARAKSALEATDDNLLR
                     ALGQRLAEALAVIGDVSTELGGYLAELPSDASTLENKLARQGELRALTRKYAADIDGV
                     LAWARESRDRLAQLDVSEETLAGLKRQVADLESQVATAAIGLSKARTKAAKGLAKAVT
                     AELSGLAMADANFTVAVSPLTVRGDDSAPITLPSGEMVHAGSDGADAVEFGFSAHRGT
                     DVLPLNKSASGGELSRVMLALEVVLAASVEGTTMVFDEVDAGVGGRAAVQIGRRLSRL
                     ARSHQVIVVTHLPQVAAYADIHLVVDSAGRNNASGVRRLDDEDRVAELARMLAGLGDS
                     DSGRAHARELLSAAQEDREQVS"
     gene            complement(2489..>2625)
                     /gene="ppnK"
                     /locus_tag="VGP27_11105"
     CDS             complement(2489..>2625)
                     /gene="ppnK"
                     /locus_tag="VGP27_11105"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216211.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEV7851888.1"
                     /translation="VPAGGRLEVTRCGTPLKWVRLDSAPFTDRLVRKFRLPVTGWRGQ
                     "
BASE COUNT          417 a          900 c          855 g          453 t
ORIGIN      
        1 tgaggaccgg ctgatactcc ttgatgaacg gcttcagtgc cttcaggtcg taggccgcgt
       61 cgggttcttc cgccacgatc acgacatgac ggcgatgaag atcgacatcg atatcgggga
      121 tgccgatgcc gtcgatgagc aaagggctct cgctgcggat gaactcgatc gtgttgccgg
      181 cgaaagcctc caggtgggcg accaggccgc tcttggcctc gtgcatcagt tcgtggatct
      241 cgtggtcggt gcgctcggtg ccgtgggcca gcctgcggtc gcccgcatac accccgccgt
      301 cgtacagccg gacccgggcg ccgtccttga tgcgcttgaa gatttcgggg ccggtctcgt
      361 cgatcaaggt gacgccgttg ttgaccagta cctcaggtcc gaggttcggg tagcggccgg
      421 atatcgaggg cgacgcattg accacggcgg cgatttcggc ttcgaccagg gcatccgcgg
      481 tgatgcggtc gaggtccagc gcgtcgatca ccacgatgtc tcctggtccg acgcgccgta
      541 acagtcgatc gatgtcgcgg tccacgcggg ccgtgccggt aatgcccggc cgtgggctgg
      601 cagtgcggga tagcagcgct gacatcttca tatcggcgat tctgtcgggc aaccctcaac
      661 tagaggtgga ggcgcgccgt accaccagcc tcacaagttt cttctgtcac acgggtcaca
      721 ggtcaggaca cctgctcgcg gtcctcctga gccgcgctga gcagctcgcg ggcatgagcc
      781 cttccgctgt ccgagtcgcc gagtccggcg agcatgcggg caagctcggc gacccgatcc
      841 tcgtcgtcga gacgacggac accgcttgcg ttgttgcggc ccgcactgtc gacgacgagg
      901 tggatgtcgg cataggcggc gacctgcggc aggtgcgtga cgacgatgac ctggtggctg
      961 cgcgccaatc gcgacagacg ccgcccgatc tgcaccgcgg cgcgaccgcc gacgcctgcg
     1021 tcgacctcgt cgaaaaccat cgtcgtacct tcgaccgacg cggcgaggac gacttcgagc
     1081 gcaagcatga cccgggacag ctcaccgccg gacgcgctct tgttcagggg cagcacgtcg
     1141 gtaccccgat gcgccgagaa tccgaattca acggcgtcgg cgccgtcgga tcccgcatgc
     1201 accatttcac ccgacggcaa tgtgatgggc gcggagtcgt cccctcgaac ggtcagcggt
     1261 gaaaccgcga cggtgaagtt ggcgtcagcc atcgccagtc cggacagttc ggccgtcacc
     1321 gctttcgcga gtcctttggc cgccttggtg cgcgccttac taagtccgat tgcagcggtt
     1381 gcgacttggc tttccagatc cgcgacctga cgcttcaggc cggccaacgt ctcctcggaa
     1441 acgtccagtt gtgcgagccg atcccgcgac tcgcgcgccc aggccaggac accatcgatg
     1501 tctgcggcgt acttccgggt cagagcccgc agctcgccct gcctcgccag cttgttctcc
     1561 aaagtacttg catcgctggg caactcggca agatagccgc caagctccgt ggacacgtcg
     1621 ccgattacag ccaacgcctc cgcgagccgc tgccccaacg cccgcaacag gttgtcgtcg
     1681 gtggcctcca acgccgactt ggcccgggcg acttcgtggg acgccgatgc ggcatcggca
     1741 tcggcggtgt cgtcgagagc cccggcaagt gccgtgcgcg cgtatcgggc ggcctcccga
     1801 agtgcgtcga gttcggagag ccgcctgatg tcgtcaacga gggcgtcgtc ctcgcccggc
     1861 cgggggtcga cggcatcgat ctcaccgagg ccgaacgtca accggtccgc ctcctgcgcc
     1921 atttcccttg cccgcgcggt tctttcagca agatcgcggc gcgccgccag ccacgactcg
     1981 cgcacagtgc gataccgctg cagcagcgca ccgacgtcgg cgaagcgatc cagcgcggca
     2041 cgctgctcgt cgccgcgcat cagtcgcaac tggtcgtttt gtccgtgcag tgtgaggagt
     2101 tcggtggtga acgtgctcag agacttcgcg ggaacactgc gaccacccag gtaggcccgc
     2161 gaggggccgg cacggctgac cgagcgcgcc gcgatgacgc tgccgtcctc gtcgcggtcc
     2221 gccccggagg cgtcaagaat cttgtcgatc tgggcggcca ccccatcgcc gagttcgacc
     2281 gtgctgaacc ggccttccac gacagcgcgg tcggcgcccg accggatccg ggtggcgtct
     2341 gcccgcgcac cgccgagaag gtgcagtccg gttaccacca tcgtcttacc ggtgccggtc
     2401 tcgccggtca gcacggtcag accctggtcg aattccgctg tcgcggtgct gattgcgccg
     2461 agcgactcga tgcggatctc ggaaagcact actgtccgcg ccagcccgtg accggcagcc
     2521 ggaacttgcg aaccaggcgg tcggtgaacg gcgcgctgtc cagccgcacc cacttcaacg
     2581 gcgtgccgca gcgcgtcacc tcgagccgac cgcctgcggg cacga
//
LOCUS       DASYLX010000456         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_789084,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000456 DASYLX010000000
VERSION     DASYLX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789084"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VGP27_11110"
     CDS             complement(<1..98)
                     /locus_tag="VGP27_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV7851889.1"
                     /translation="MAKKDGAIEVEGRVVEPLPNAMFRIELENGHKV"
     gene            347..2050
                     /locus_tag="VGP27_11115"
     CDS             347..2050
                     /locus_tag="VGP27_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7851890.1"
                     /translation="MTRSSVEPRKPAVLTVDDDPAVSRAVARDLRRHYGERYRIVRAE
                     SGADAIDTLKELKLRGDTVAVFVADYRMPQMSGIEFLEKAMDFFPLARRVLLTAYADT
                     HAAIDAINVVDLDHYLLKPWDPPEEKLYPVIDALLEDWRAVGDRAMPYTKVIGHRYND
                     RSWEVRQFLARNEYSFRAVNADEPKGQQLLEAAGIDGSRLPVVITEKGETLVDPTDVE
                     LATLLGLSTSPQLQMYDLAVIGGGPAGLAAAVYGASEGLETVLIERTSTGGQAGRSSK
                     IENYLGFPLGVSGAELTTAARRQAEKFGAEVITTQEAGKLEVNGVGAAHSIRLNDDNT
                     IGARAVILATGVEYRQLQITGCWDDPDDSACNYIGRGVYYGASVSDNTECAGEDVYIV
                     GGANSAGQAAMYMSQTAKSVTMLIRGPSLEAGMSQYLVDRILKAPNITVRTCTEVVDT
                     VAADGHLSGLVLLDKRTDETQSVSCDRMCCFIGATPRTDWLEEAGVARDDYGFILSGP
                     DVMKVSGWMLDRPPHHLETSVPGVFVAGDVRSDSAKRVAAAVGEGSMAVMLVHRYLAE
                     A"
     gene            2062..3555
                     /locus_tag="VGP27_11120"
     CDS             2062..3555
                     /locus_tag="VGP27_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7851891.1"
                     /translation="MGDECVRDELRTLFLFEDLSDEQLDMLCAYGHIDTFPAGPLCLE
                     GEPASCFYVMIDGELIMSKRSGGVDIQTGRTSQRGVYFGAWSAYVPGEEHIYEASVRL
                     TKPSRCFVLDADAFARFMQSQFPMAVHLLEGHKVGGRRQSQIIGQREKLLALGTITAG
                     LTHQLNNPAAATARAVADLREGVGHMRHKLAMVAEAKFTPEALRVLVNIQDQVAEQVA
                     KNTDVELTALEASDREDQIGDWLEDHGIAGAWDYAPTFVEAGLDTDWLERVSSLVDDV
                     DATASLQGAIGWLKYTIDNELRMNEIAEASKRISALLAGAKQYSQMDRGAYGSADVHE
                     LLRSTIMMFGDKIGMEGKGRPVTLVKDMDKTLPELQCYPGDLNQVWTNIMENAIQAMD
                     GRGTLTVRTARENDQMIRVEICDDGPGIPEDVIDRIFTPFFTTKPFGKGTGLGLDLAW
                     RIVVEKHGGNISVQSKPGDTRFIVCLPLVAPAPMTPTPGELSKAGTE"
     gene            3585..>4428
                     /locus_tag="VGP27_11125"
     CDS             3585..>4428
                     /locus_tag="VGP27_11125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7851892.1"
                     /translation="MTTPDLGTFGAFGHHSMWQQLSPEQLREIEALGYGAIWAGGSPA
                     AELSWVDPILDQTDKLKLATGIVNIWTAEAGPVAESFHRIERAYPGRFLLGIGVGHPE
                     AHQQYRKPIDALTDYLDKLDEYGVPKDSRVVAALGPKVLKLSAERAAGAHPYLTTPEH
                     TAEARKLIGPEALLAPEHKAVLTTDPEKARAVGRKALEIYLNLANYLNSWKRLGFTDE
                     DVAKPGSDRLVDAVVAYGTPDEIAARLKEHITAGADHVAVQVLTGPDKLVPALAELAG
                     PLGLS"
BASE COUNT          819 a         1437 c         1448 g          724 t
ORIGIN      
        1 accttgtgtc cgttctccag ctcaatgcgg aacatcgcat tgggcagggg ttcgaccacg
       61 cggccctcga cctcgatggc accgtctttc ttggccattg ttgttcggag atcctccgtg
      121 tccttctggt tctttggcgc ctggcggcgc agaattcgtt ccgactacaa aacgtgagcc
      181 ggtagctaga aattccagaa ctggggcacg caagagccgg cgcgtatacc gcaccgttgg
      241 tccatactac ctgctcgacg gcgtgcccca aaatcgccga acggccacct cagcgctgtc
      301 ttgtcgacct catattacct ggacgaccgg cgcataattg atcgcgatga cgcgatccag
      361 tgtggagcca cgtaaacctg ccgtcctcac cgtcgacgac gaccccgcag tatcgcgggc
      421 ggtcgcccgc gacctccgcc gacactacgg cgagcggtac cgcatagtgc gcgccgagtc
      481 cggggccgac gcgatcgaca cgctgaagga actcaagctg cgcggtgaca cggtggctgt
      541 cttcgtcgcc gattaccgca tgccccagat gagcggcatc gagtttctcg agaaggcgat
      601 ggacttcttc ccgctggcgc ggcgggtgct gctgaccgcc tacgcagaca cccacgccgc
      661 catcgacgcc atcaacgtcg tcgacctcga ccactacctg ctcaagccgt gggacccgcc
      721 ggaggagaag ctctatccgg tgatcgacgc cctcctcgaa gattggcgcg cggtgggcga
      781 tcgcgcgatg ccgtacacca aggtcatcgg tcaccgatac aacgaccggt cgtgggaggt
      841 gcggcagttt ctggcgcgca acgagtactc cttccgtgcg gtcaatgccg acgagccgaa
      901 ggggcagcaa ctgctcgagg ccgcaggaat cgacggcagc aggctgccgg tcgtcatcac
      961 cgagaagggc gagacgctgg tcgacccgac ggacgtcgag ctggcaacac tgctcggcct
     1021 gtcgaccagc ccgcaattgc agatgtacga cctcgcggtg atcggcggcg gtccggccgg
     1081 gctcgccgcg gcggtgtacg gcgcgtcgga gggactcgag acggtgctga tcgagcggac
     1141 ctccaccggc gggcaggcgg ggcgcagttc gaagatcgag aactacctcg gcttcccgtt
     1201 gggcgtctcc ggtgccgagc tgaccactgc ggcgcgccgc caggccgaga agttcggcgc
     1261 cgaggtcatc accactcagg aggcgggaaa gctcgaggtg aacggcgtcg gagccgccca
     1321 ttccatccgc ctcaacgacg acaacaccat cggcgcgcgc gccgtcatcc ttgccaccgg
     1381 cgtcgagtac cgccagctcc agatcaccgg gtgctgggac gatcccgacg actcggcgtg
     1441 caactacatc ggccgcggcg tttattacgg ggcgtcggtg tcggacaaca ccgaatgcgc
     1501 cggcgaggac gtctacatcg tcggtggtgc gaactcggcg ggtcaggccg ccatgtacat
     1561 gtcgcagacc gccaagtcgg tcacgatgct gatccgcggc ccgtcgctgg aggcgggcat
     1621 gtcgcagtac ctggtcgatc gcatcctcaa ggctccgaac atcacggtcc ggacctgcac
     1681 cgaagtcgtc gacaccgtcg cggcggacgg ccacctgtcc ggtttggtcc tcctcgacaa
     1741 acggacggat gaaacacagt cggtgtcatg cgaccggatg tgctgcttca tcggcgcgac
     1801 gccgcgcacc gactggttgg aggaagcggg agtagcccgc gacgactacg gcttcatcct
     1861 gtccggtccg gacgtgatga aggtcagcgg atggatgctg gaccgtccgc ctcaccacct
     1921 ggaaacaagt gtgcctggtg tgtttgttgc aggagatgtg cgctcggatt ccgcgaagag
     1981 ggtggcggcc gccgtcggcg aagggtcgat ggcagtgatg ctggtgcaca ggtatctggc
     2041 ggaagcctag agaggaccac catgggcgac gaatgcgtcc gcgacgagct caggaccttg
     2101 ttccttttcg aggatttgtc cgacgagcag ctcgacatgc tgtgcgctta cggtcatatc
     2161 gacacgttcc ccgccggtcc gctgtgcctc gaaggcgagc ccgcgagctg cttctacgtg
     2221 atgatcgacg gcgaactgat catgtcgaag cgctccggcg gcgtggacat ccagaccggt
     2281 cgcacgtcgc agcgcggcgt gtatttcggc gcctggtcgg catacgtccc cggcgaagag
     2341 cacatctacg aggcctccgt acgcctgacg aagccatctc gctgctttgt cctcgacgcc
     2401 gacgccttcg cgcggttcat gcagtcgcag ttccccatgg ccgtgcacct gctcgagggc
     2461 cacaaggtgg gcggcaggcg acagagccag atcatcggcc agcgcgagaa gctgttggct
     2521 ctgggcacca tcaccgccgg gctgacccat cagctcaaca atcccgctgc cgcgactgcg
     2581 cgcgcggtgg ccgatctccg cgagggtgtc ggccacatgc ggcacaagct ggcaatggtg
     2641 gccgaggcca agttcacgcc cgaggcgctg cgcgttctgg tgaacattca ggaccaggtc
     2701 gctgagcagg tggccaagaa cacggacgtg gaactcaccg ctctcgaggc gtccgaccgc
     2761 gaggaccaga tcggtgactg gctcgaggac cacgggatcg cgggcgcctg ggactatgcg
     2821 ccgacgttcg tggaggccgg gctggacacc gactggctgg agcgcgtctc gtcgttggtc
     2881 gacgacgtcg acgcgacggc ctcactgcag ggcgcgatcg ggtggctgaa gtacaccatc
     2941 gacaacgagc ttcgaatgaa cgagatcgcc gaggccagca agcgcatctc ggcactgctc
     3001 gccggcgcga agcagtattc gcagatggac cgcggggcct acgggagcgc cgatgtccac
     3061 gaactgctgc gcagcacgat catgatgttc ggcgacaaga tcgggatgga gggcaagggc
     3121 cggcccgtca cgctggtcaa ggacatggac aagacgttgc ctgaattgca gtgctacccc
     3181 ggcgatctca accaggtgtg gaccaacatc atggaaaacg cgattcaggc gatggacggt
     3241 cgcggcaccc tgaccgttcg caccgcacgc gagaacgatc aaatgattcg cgtcgagatc
     3301 tgcgacgacg gccccggtat ccccgaagac gtcatcgacc gcatcttcac tccgttcttc
     3361 accaccaagc ccttcggcaa gggcaccgga ctcgggttgg acttggcgtg gcgcatcgtc
     3421 gtcgagaagc acggcgggaa catctccgtg caatcgaagc cgggcgacac gcggttcatc
     3481 gtgtgcctgc ccttggtggc gccggcgccg atgacaccga cgcccggtga gctctcgaag
     3541 gccggcacgg aatagcttgc gccgcgtcgt aggttggaga aaccatgacc actccagatc
     3601 tcggcacgtt cggcgcgttc ggtcaccatt cgatgtggca gcaactgagt cctgagcaac
     3661 tgcgagaaat cgaggctctc ggctacgggg ccatctgggc gggcggctcg ccggcggcgg
     3721 aactgtcgtg ggtcgatccc atcctcgatc agaccgacaa gctgaagctg gccaccggga
     3781 tcgtcaacat ctggaccgct gaagcggggc cggtcgcgga gtcgttccac cgcatcgaga
     3841 gggcctaccc cgggcgcttc ctgctcggaa tcggtgtcgg gcatcccgag gcgcaccagc
     3901 agtaccgcaa accgatcgat gcactcaccg attatctgga caaactcgac gagtacggcg
     3961 tgcccaagga cagccgcgtg gtagccgcgc tcggccccaa ggtgttgaag ctgtcggcgg
     4021 aacgcgccgc gggagcgcat ccctatctca cgacaccgga acacaccgcc gaagcgcgca
     4081 agctgatcgg gccagaggcg ctcctcgcgc ccgagcacaa ggcggtgctg accaccgacc
     4141 cggagaaggc ccgcgccgtc ggacgcaagg cgctcgagat ctacctcaac ctggccaact
     4201 acttgaacag ctggaagcga ctggggttca ccgatgaaga cgtggcgaag cccggaagcg
     4261 atcggctggt cgacgccgtc gtcgcctacg gcacccccga cgagatcgcc gcgcggctga
     4321 aggagcacat cacggccggc gcagaccatg tggcggtgca ggtgctgacc ggacctgaca
     4381 agctggtccc tgcgctcgcc gaactcgccg ggccgctcgg cctttcgt
//
LOCUS       DASYLX010000457         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2873884,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000457 DASYLX010000000
VERSION     DASYLX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873884"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGP27_11130"
     CDS             complement(<1..254)
                     /locus_tag="VGP27_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851893.1"
                     /translation="MGTEEAERPNKTDRTPQTIRRFSVLIALFWLALAVVTNVFVPQL
                     ETVAEAHNVSLSPQDSPSLQASKRIGKVFGEFDSDSSAMIV"
     gene            complement(263..709)
                     /locus_tag="VGP27_11135"
     CDS             complement(263..709)
                     /locus_tag="VGP27_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7851894.1"
                     /translation="MPRLSLGSRLSRRWMLLVAVFVVAIAGFAVYRLNGIFGSHDVTS
                     TPSGAANDIVPFNPKHVVLEVFGPPGTVATITYLDVNAQPQRADDATLPWAYDTTTTQ
                     PAVFVNVSAQGDSDSIGCRIKIDDVVKDERTVNTLNAFTYCLDKSG"
     gene            1022..2233
                     /locus_tag="VGP27_11140"
     CDS             1022..2233
                     /locus_tag="VGP27_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851895.1"
                     /translation="MSFSLELSEDVIQVRDWVHEFAESVLRPAAAEWDEREETPWPII
                     QEAAKVGLYSVDLFATQAAEPTGLGMLVVFEEMFWGDAGIALSILGTGLAAASLAATG
                     TPEQLGEWLPQMFGTPEDPLLASFCSSEPGAGSDVGAILTRARYDEANDEWVLNGVKT
                     WATNGGIANVHIVVASVYPELGSRGQATFIIPPNTPGFKQGQKFKKHGIRASHTAEVI
                     LEDVRIPARLIVGGKDRFDERIARNREGRSAAAQAAMKTFERTRPSVGAMALGVARAA
                     YEYSLEYAQQREQFGRKIADFQAIAFKLADMKSRVDAARLLVWRAGWMARNNQPFTNA
                     EGSMAKLVASETAVYVTDEAIQILGGNGYTRDYPVERMHRDAKIFTIFEGTSEIQRLV
                     MGRAITGLPIR"
BASE COUNT          505 a          798 c          816 g          470 t
ORIGIN      
        1 acgatcatcg ccgaactgtc ggagtcgaac tcgccgaaca ccttgccgat gcgtttactg
       61 gcctgcaacg acggcgaatc ctgcgggctc agcgacacgt tgtgcgcttc ggcgacggtc
      121 tccaactgcg gcacgaaaac attcgtgacc accgccaagg ccagccagaa cagcgcaatc
      181 aggaccgaga agcgccggat cgtctgtggg gtccggtcgg tcttgttcgg gcgttcggcc
      241 tcctccgtgc ccatccgagt gctcatccgg acttgtccag gcagtaggtg aaggcgttca
      301 gcgtattaac cgttctctcg tccttgacga cgtcgtcgat cttgattcgg cagccgatcg
      361 agtcgctgtc gccctgggcg gagacgttga cgaagacggc cggttgagtg gtggtggtgt
      421 cgtaggccca cggcagcgtc gcgtcgtcgg cccgttgcgg ctgggcgttc acgtccaggt
      481 aggtgatggt cgcgaccgtg cccggcgggc cgaagacctc aaggacgacg tgcttggggt
      541 tgaacggcac gatgtcgttg gcggcgccgc tgggggtgga cgtgacgtca tgcgagccga
      601 agatgccgtt gaggcggtac accgcgaatc ccgcgatcgc gacgacgaac accgccacca
      661 gaagcatcca gcgtcggctc aatcggcttc cgagcgaaag ccgcggcatc cgtgaccctt
      721 tcgacgagcg gcgacctgca gacggcaaca tttctacggg gtcggcccca tatcaatcag
      781 gttgacagta cgagacggta ccggccgcca caacaaatac ccctcaggca ttgtgccggg
      841 cgaagccgga aacggcgcgc ttcgatcgtc gaggagaggg cggcgcctcc gacttgcccg
      901 tggacggccg caagtgacct actccatagt ggctgaactt actccctggt aagtatcagt
      961 gtacggttgt tcgccgaagt ggtactactc ccgtgcgcgt gcataagaag gatgagcggc
     1021 tgtgtcgttt tctctcgagc tttccgagga tgtaattcag gtcagggact gggttcacga
     1081 gttcgcggaa tccgtactgc gtccggcggc agcggagtgg gatgaacgcg aggaaacccc
     1141 ttggccgatc atccaggagg cggccaaagt gggcctctac tccgtggact tattcgcaac
     1201 ccaagcggcc gaaccaaccg gtctcggaat gttggtcgtc ttcgaggaga tgttctgggg
     1261 cgatgcaggc atcgcattgt ccatcctggg cactgggctg gcagccgcgt cgcttgccgc
     1321 taccggcaca cccgaacagc tcggcgaatg gctgccgcag atgttcggca cgccagagga
     1381 cccgcttctt gcgtcgttct gctcgtcgga acctggggcc ggctcagatg tgggcgccat
     1441 tcttacccgt gcccgatacg acgaagccaa cgacgagtgg gtgctaaacg gtgtcaaaac
     1501 atgggctacc aatggcggga tcgccaacgt gcacatcgtt gttgcgtccg tctatcccga
     1561 actgggcagc cgcgggcaag ccacattcat catcccgccg aacacaccgg gcttcaaaca
     1621 gggacaaaag ttcaaaaagc acgggattcg ggcgtcgcac accgccgagg tgatcctgga
     1681 agacgttcgc atcccggctc ggctgattgt cggcggcaaa gacagattcg acgaacggat
     1741 cgctcgcaac cgtgaagggc gaagtgccgc agcgcaagcg gcgatgaaga ccttcgagcg
     1801 gactcggcct tcggtggggg cgatggcgct gggcgttgcc cgcgcggcct acgaatactc
     1861 actcgagtac gcgcagcagc gagaacagtt cgggcgaaag atcgctgact tccaggcgat
     1921 tgcgttcaaa ctggccgaca tgaagtctcg tgtcgacgct gccaggttgc tggtgtggcg
     1981 ggcgggttgg atggcgcgta acaaccagcc gttcaccaac gccgagggct cgatggccaa
     2041 actcgtggcg agcgaaaccg ccgtctacgt gaccgatgag gccatccaga tcctcggcgg
     2101 caacggttat acgcgtgact acccggtcga acgcatgcac agggacgcga agatcttcac
     2161 catcttcgaa ggcaccagtg agatccagcg actcgtcatg ggcagagcga tcaccggact
     2221 gccgatcagg tagggactcg ggacggccgc cgacctcagg caaagcgccg tgcctgcatc
     2281 cgcgaaacgg aattccagca gaccttcact cgcgaacttc tcctgcttga attccgtttc
     2341 gggaagaatg cgacgcgaac ggtgacgaag ccacgctttg ccgtcccgac caccgatcga
     2401 aagggccatc cctatgtcca accatttcac cggcctgagc ctcgggcctc cgcttggcga
     2461 ccagcgactg gacctgtgcg acctctatgc cttcgtgtcg ccctcagacc cgtcgcggac
     2521 ggtcatcatc ctcaacgcca acccgctcgc cgaagccctc caccccggtg cgatttaccg
     2581 tgtggcggt
//
LOCUS       DASYLX010000458         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2214447,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000458 DASYLX010000000
VERSION     DASYLX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214447"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGP27_11145"
     CDS             complement(<1..485)
                     /locus_tag="VGP27_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7851896.1"
                     /translation="MPVQEEFIAQAAPFRAELIAHCYRMLGSVHDAEDLVQETYLRGW
                     RGYENFEERAALRTWLYRIATTACLRALENRARRVLPAGLGAGAIDPEVDLDGAGFAH
                     PWLEPLPDTYTPESAIAARHSVRLAVVTAMQELPARQRAVLILRDVVQFSAAEVAELL
                     ET"
     gene            643..1407
                     /locus_tag="VGP27_11150"
     CDS             643..1407
                     /locus_tag="VGP27_11150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851897.1"
                     /translation="MDDFEELNPLGGTTAIVTGASRGLGRSVASALTAAGVHVVGVAR
                     SAARLADVTEELGNGFTAVTADAADPATARRLIDAHRPTTVVLCAGAAPTMSGLQDQT
                     WETFSQNWNVDVAQAFHWTRAALTRPLAPGSSVIALSSGAALKGSPLSGGYAGAKATV
                     RFITSYAAGESERNGLGIEFLSLLPQLTPDTDLGLAAVQAYAGRQGVDVDAFVQAMAP
                     VLTPEMVGKTVLEIACGRLRGHRAYALASAGASALD"
     gene            1422..2123
                     /locus_tag="VGP27_11155"
     CDS             1422..2123
                     /locus_tag="VGP27_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEV7851898.1"
                     /translation="MTENTMQPIVSAPEWEAALGELLVKEKELTRARDALAAARRRMP
                     WLAVDKHYEFDGPAGRASLLDLFGGRRQLIVYRAFFEPGVHGWPDHACPGCSMLADQV
                     GHVAHLNSRDTTLVFASRGPQADIERVKARMGWEMPWYTITDDFDADFGVDEWHGTNA
                     FIRDEERIYRTYFVNNRGDEAMGNLWSYLDITALGRQENWEDSPEGYPQSAPYQWWNW
                     HDEYQTRELSDRGAQ"
     gene            2120..2530
                     /locus_tag="VGP27_11160"
     CDS             2120..2530
                     /locus_tag="VGP27_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851899.1"
                     /translation="MIPQATVAGRRACALLTASSAVLHGIAALHADMLAAAVLMSAMG
                     IACLFCARELWMHGALRTWTLVAVMNIAMIAVHTPFASGHQHGGSVALAAMPTHHSMA
                     MTAATALAAMESMIATAVVYRLSRHHRFLVHASR"
     gene            2619..>3378
                     /locus_tag="VGP27_11165"
     CDS             2619..>3378
                     /locus_tag="VGP27_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7851900.1"
                     /translation="MVGDTGPSRDEAGSGIEPPVAPRVRRTFSLLERFAPAVGSRWAV
                     ELWCTPPAVEMSLRMPPGVTAGEPVEATWSGHRIAGESWGDGPAVYLVHGWGGCRAHL
                     GVFVKPLVEAGHRVIAFDLPSHNDSDPGELAPGRTTIVECAEAVRAFVHAHGPARGII
                     AHSLGAKAAALAVERGMIVERMVFLAPMGDFSLYLDLFAEKHGFGPCIRTGLQRRLDR
                     RLNMPLFDTDISAVATGLDDPPPLLVVHDPDDPDS"
BASE COUNT          568 a         1107 c         1134 g          569 t
ORIGIN      
        1 gtctccagca actcggcgac ctctgccgcg ctgaactgca caacgtcgcg caggatcagt
       61 acggcgcgct gacgggcggg aagctcctgc atcgcggtga ccactgccag ccggacgctg
      121 tgtcgcgccg cgatcgccga ctcgggcgta tacgtgtcgg ggaggggctc cagccacggg
      181 tgagcgaagc ctgcaccgtc gaggtcgacc tccgggtcga tcgcgccggc gcccagtccc
      241 gcaggcaaca cccgacgggc ccggttttcc agagcgcgca ggcacgccgt ggtcgcgatg
      301 cggtacagcc aggtacggag cgccgcccgc tcctcgaagt tctcataccc acgccatccg
      361 cgcaggtatg tctcctgcac caggtcctcg gcatcgtgga ccgaaccgag catgcgatag
      421 cagtgcgcta tcagctcggc ccgaaacggc gcagcctgcg cgatgaactc ttcctgcacc
      481 ggcacggtga ctctcctcgg gtcgtcggtg atcgctgatt gcgtcaccaa tacagatcca
      541 tcggccggcc gaaaatcatc gatcaccgca ccgatgaatt tcggcggcgg gacggatctg
      601 aactagtgaa gacataaaca cctgacacag gaggtaggac tcgtggacga cttcgaagag
      661 ctcaatccgc tcggcggcac caccgcgatc gtgactggtg ccagccgtgg tctcgggcgg
      721 agcgtcgctt ccgctctgac cgcggcgggc gtgcatgtgg tcggtgtcgc gcggtccgct
      781 gcgcggctcg cggacgtgac ggaggaactg ggcaacggct tcaccgctgt aacggccgac
      841 gccgcggacc ccgcgaccgc acgccgtctg atcgacgcgc accgacccac caccgtggtg
      901 ctgtgtgccg gcgccgctcc gacgatgtcg ggcttgcagg accagacgtg ggagaccttc
      961 agccagaact ggaacgtcga cgtcgctcag gcattccact ggacccgcgc ggcactcacc
     1021 cgcccgcttg caccgggcag ctcggtgatc gcattgtcca gcggtgccgc gctgaagggt
     1081 tcgccgctca gcggcggcta tgcgggagcg aaggccacgg tcaggttcat caccagttat
     1141 gccgcaggcg aatcggagcg caatggcctc ggcatcgaat tcctttcctt gcttccgcaa
     1201 ctgacgccgg acaccgacct cgggctggcg gcagtgcagg catacgccgg gcggcaaggg
     1261 gtcgacgtcg acgcgttcgt gcaagcgatg gctcctgtgt tgacacccga gatggtcggc
     1321 aagaccgtgc tcgagatcgc ctgcggtcgg ctgcgcggtc accgcgccta cgcgctcgct
     1381 tccgccggcg cctcggcact tgattgagag gctgaaagat catgacggag aacacaatgc
     1441 aaccgatcgt gtcggcaccc gaatgggaag ccgctctcgg cgaattgttg gtgaaggaga
     1501 aggaactgac ccgcgcacgc gacgcgctgg ccgccgctcg gcgccgaatg ccgtggctgg
     1561 ccgtggacaa gcactacgag ttcgacggac cggcaggcag agcaagcttg ctcgacctgt
     1621 tcgggggccg tcggcagctg atcgtctacc gcgccttctt cgaacccggt gtgcacgggt
     1681 ggcccgatca cgcgtgcccg ggttgttcca tgctggcgga ccaggtcggc catgtggcgc
     1741 acctgaattc gcgcgacacc acgctggtct tcgcgtcccg cggtccgcag gccgacatcg
     1801 agcgggtgaa ggcccgaatg ggctgggaaa tgccttggta cacgatcacc gacgatttcg
     1861 acgccgactt cggagtcgac gaatggcacg ggacgaacgc gttcatccgt gacgaggagc
     1921 ggatctaccg cacgtacttc gtcaacaacc gcggtgacga ggcgatgggc aacctctgga
     1981 gctacctcga catcaccgca ttgggccggc aggagaactg ggaggactct cccgagggct
     2041 acccgcagag cgcaccctac caatggtgga actggcacga cgagtaccaa actcgcgagt
     2101 tgtccgaccg cggagcacaa tgatccctca ggccacggtt gcaggccgcc gggcgtgcgc
     2161 gcttctcacg gcgtcttcag ccgtgctgca cgggattgcg gccctacacg ccgacatgct
     2221 tgcggcggcc gttctgatgt cggcgatggg aatcgcgtgc ctcttctgtg cgcgcgaact
     2281 ctggatgcac ggcgcgcttc ggacatggac gcttgtcgcg gtgatgaaca tcgcgatgat
     2341 cgcggtgcat actccgttcg cgtcgggcca tcagcacggc ggttcagtgg cactcgccgc
     2401 gatgccgacg catcattcaa tggcgatgac cgccgccacg gcgctcgccg ccatggagtc
     2461 gatgatcgcg accgccgtcg tctaccggct cagtcggcac catcggtttc tcgtgcacgc
     2521 aagccggtga gggaaggaga ttgctccagg cgccggtcat cggcaggcaa gtcctgatca
     2581 agtaacctta ctcgcgagta agaaagaggg ggcttgtgat ggttggcgat accgggcctt
     2641 ccagggacga ggcgggctcc ggcatcgagc ctcccgttgc gccgagggtg cgccggacat
     2701 tttcgctgct ggagcgtttt gcgcccgcgg tgggttcgag atgggcggtc gagctgtggt
     2761 gcaccccgcc cgccgtggag atgagtctgc gcatgccgcc gggcgtcact gcgggtgagc
     2821 cggtcgaggc gacctggtcc ggtcaccgga tcgccgggga gtcctggggt gatggcccgg
     2881 cggtctacct ggtgcacggt tggggcggct gccgcgcaca cctgggagtc ttcgtcaaac
     2941 cgctcgtcga agcgggtcac cgggtcatcg ccttcgatct gccgagccat aacgattcgg
     3001 atccgggcga actcgcgccc ggacgcacca ccatcgtcga gtgcgccgaa gcggtgcggg
     3061 cattcgtgca tgcgcacggg ccggcgcgcg gaatcattgc ccattccctc ggagccaagg
     3121 ccgccgcgct ggcggtcgaa agagggatga tcgtagaacg catggtgttc ctggccccga
     3181 tgggcgactt ctcgttgtat ctggacctgt tcgccgaaaa acacggcttc gggccgtgca
     3241 tccggaccgg cttgcagcgc cgcctcgacc gacggctgaa catgccgctg ttcgacaccg
     3301 acatttccgc ggtggcgacc ggtctcgacg atccgccgcc gctcctggtg gtgcacgacc
     3361 ccgacgatcc tgacagcc
//
LOCUS       DASYLX010000459         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2434434,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000459 DASYLX010000000
VERSION     DASYLX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434434"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /gene="ligA"
                     /locus_tag="VGP27_11170"
     CDS             complement(<1..1755)
                     /gene="ligA"
                     /locus_tag="VGP27_11170"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV7851901.1"
                     /translation="MAQQSEDAPDADLRRRWQELADEVRAHQFRYYVKDAPVISDAEF
                     DTLLRELQALEDQHPELRTPDSPTQLVGGAGFATDFTSAEHLERMLSLDNVFTPDELA
                     AWAGRIKGEIGDDAHYLCELKIDGVALALVYRDGKLVRAATRGDGRTGEDVTLNARTI
                     DDVPQKLTGSKEFPVPKVLEVRGEVFFRIADFEELNAGLVAEGKPPFANPRNSAAGSL
                     RQKNPAVTARRRLRMICHGLGRTEGFRPKTLHDAYRALKSWGLPVSDHTVRVQGIEAV
                     TERIAYWGEHRHDVEHEIDGLVVKVDDVALQRRLGSTSRAPRWAIAYKYPPEEATTKL
                     LDIRVNVGRTGRVTPFAWMEPVKVAGSTVSQATLHNASEVKRKGVLIGDTVVIRKAGD
                     VIPEVLGPVVDLRDGSEREFVMPTTCPECGTKLAPAKEGDADIRCPNTRSCPAQLRER
                     VFHVAGRGAFDIEGLGYEAGIALLQAGVITDEGDLFTIRSEDLLRTDLFTTKDGKLSA
                     NGKRLLVNLHEAKAQPLWRVLVALSIRHVGPTAARALAAEYGSLEAIMSASEEELSVV
                     EGVGPTIAAAVTEWFSVDW"
     gene            1906..2700
                     /locus_tag="VGP27_11175"
     CDS             1906..2700
                     /locus_tag="VGP27_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7851902.1"
                     /translation="MDKVFKALADPGRRLLLDRLRQDNGQTLGQLCEYLEMARQSVSQ
                     HLAVLESANLISSVRRGRLKLHYLNPVPLQEIAYRWIQPFETYRLHALHEVKLQAEEA
                     HMTGTTDKPTFVYVTYIHSSPEKVWEALTSAELTAAYWGHSNVSDWQKGSRWEHIRTD
                     GSGVVDVAGEVIEATPPRRLVITFGGADEAGQPTVVTFDVEPHHDIVRVTVTHEGLDD
                     PSDYRAVSTGWPAVLSNLKSLLETGRVLPQAPWEMHADLRAQQMSG"
     gene            2717..3121
                     /locus_tag="VGP27_11180"
     CDS             2717..3121
                     /locus_tag="VGP27_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV7851903.1"
                     /translation="MPHPIMFRDDDPDLAEVRKIALSYPDAFEKVSHGRPAFFVSKMF
                     AMYGGSAKPATKGDYISYPHSIMVKVDESDRRALGQDTRFFYPAYLGPAGWLGLDLTA
                     TKAIDWGEVGELIDASFRMVAPKKLIKQLDHA"
     gene            3155..4765
                     /locus_tag="VGP27_11185"
     CDS             3155..4765
                     /locus_tag="VGP27_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HEV7851904.1"
                     /translation="MSFASPYPDVEIPNVSVYEYLFGNLDDADADNVALVDTKSGNAI
                     SYRDTVGNINSFAGALADRGIAVGDVVGLLAPNSAAFAIAFHGILRAGATATTINVLF
                     TAKDIVKQLTDSKATMLVTVTPLLSQAREAAAAVGISDEQLVVLDGADGHPSAADLLG
                     AGATPPEVSFDPETHLAVLPYSSGTTGNPKGVMLTHRNLVANIAQIRAAQDVEADDAV
                     IAFLPFFHIYGMTVLLNAVLRARARLVMMPSFDLEEFLANIQNHRVTVAFIAPPVAVA
                     LAKHPLVDKYNVSTLRAVLSGAAPLDEDLGHAVADRIGCPVVQGYGMTELSPVSHCTP
                     FDGGMRLVGSAAPIGAAGWTAANSSSRLIEPDSGDEIDVPAAGLSETGELWFRGPNVM
                     AGYLGNEEATRETIDDDGWLRTGDLARVDSSGCVYIVDRLKELIKYKGYQVPPAELEA
                     VLLTHPAIADAAVIGVRDADSGEEVPKAFVVKQSGAELTEADVMDFVAGEVAPYKKVR
                     QVAFIDAIPKSSAGKILRKDLRLDSTAQ"
     gene            complement(4751..5542)
                     /locus_tag="VGP27_11190"
     CDS             complement(4751..5542)
                     /locus_tag="VGP27_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851905.1"
                     /translation="MGHSTVRFAAGAGVLAASLLIAGPNPAHAVADKHGSGAHAKNDS
                     RKNGSGGPLGDVEPGVANAVEDALQVGGTADTDLTPPSMNLSVGGSDLEDPAVVTGLG
                     TESLTALRSAAVVEQPPGDNVSAAAPRAGSGYSGPPLAPFRGPKVVVGNGRTPGTHVP
                     MPGPAAEAVLTDDAMVSPAAVSAAPGVPAAIEINFPPLPPPMPPVERIRPAHLTVGEY
                     GTGTTDTVADPLAGVAGLILIPAIGAVLGYRQARAAQSLRESLRS"
     gene            complement(5621..>5731)
                     /locus_tag="VGP27_11195"
     CDS             complement(5621..>5731)
                     /locus_tag="VGP27_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV7851906.1"
                     /translation="PVCGLAGATPQWARIAIELVQEAADAIAADPAAIMK"
BASE COUNT          957 a         1883 c         1913 g          978 t
ORIGIN      
        1 ccagtcgacg ctgaaccatt cggtgaccgc cgcggcgatc gtcggtccga ccccctcgac
       61 gaccgacagc tcctcctcgg aggccgacat gatcgcctcc aggctgccgt attcggctgc
      121 cagcgcgcgg gcggccgtcg ggccgacgtg gcggatcgac aacgcgacga gcacccgcca
      181 caacggctgg gccttggctt cgtgcaggtt caccagcagc cgcttgccgt tggctgacag
      241 tttcccgtct ttggtggtga acaggtcggt gcgcagcagg tcctcgctcc ggatggtgaa
      301 caggtcacct tcgtcggtga tgacaccggc ctgcagcagc gcgattcccg cctcgtagcc
      361 gaggccctcg atgtcgaacg cgccgcgccc tgcgacgtgg aagacccgct cgcgcaattg
      421 cgccggacac gacctggtgt tggggcagcg gatgtcggcg tcgccttctt tggccggtgc
      481 gagtttcgtg ccacattcgg gacatgttgt gggcatgacg aattcgcgtt cggagccgtc
      541 acgcaggtcg acgacgggcc cgaggacttc gggaatcacg tcgccggcct tccggatcac
      601 cacggtgtca ccgatgagca cgcccttgcg cttgacctcc gacgcgttgt gcagcgtggc
      661 ctggctgacc gtcgagcccg cgaccttgac gggctccatc caggcgaacg gggtgaccct
      721 gccggtgcgc ccgacgttga ccctgatgtc cagcagcttg gtggtcgctt cttccggcgg
      781 gtacttgtag gcgatggccc atcgcggcgc ccgcgacgtc gagccgaggc gccgctgcag
      841 tgcgacgtcg tcgactttga cgacgagacc gtcgatttcg tgttcgacgt cgtgccggtg
      901 ttcgccccag taggcgatgc gctcggtgac cgcttcgatg ccctgcaccc tcacggtgtg
      961 atcggacacc ggcagccccc acgacttcag cgcgcggtag gcgtcgtgca gcgtcttggg
     1021 gcggaagccc tccgtgcggc cgagcccgtg gcagatcatc cgtagcctgc gacgggcggt
     1081 gacggcgggg ttcttctgtc gcagtgagcc tgctgcgctg ttacgcgggt tggcgaacgg
     1141 cggcttgccc tcggcgacca ggcccgcgtt gagttcctcg aaatcggcga tccggaagaa
     1201 cacctcgccg cgcacctcga gcaccttcgg caccgggaac tcctttgagc ccgtgagctt
     1261 ctgaggtacg tcgtcaatgg tgcgggcatt gagcgtgacg tcctcaccgg tgcgaccgtc
     1321 gccgcgggtg gcggcgcgca ccagcttccc gtcgcggtag accagcgcca gggcgacacc
     1381 gtcgatcttc agctcgcaca ggtagtgcgc gtcgtctccg atctcgccct tgatgcgccc
     1441 ggcccatgcc gcgagctcgt cgggagtgaa gacgttgtcc aggctgagca tgcgctccag
     1501 atgctccgcg gacgtgaagt cggtcgcgaa gcccgcgccc ccgaccagct gcgtcggcga
     1561 atcgggggtc cgcagctcgg ggtgttggtc ctcgagcgcc tgaagttcgc gcagcagcgt
     1621 gtcgaactcg gcgtcggaga tgacgggggc gtctttgacg taatagcgga actggtgcgc
     1681 acgcacctcg tcggccagtt cctgccagcg gcggcgcaga tcggcatcgg gcgcgtcttc
     1741 ggactgttgg gccagcgcct tttgcgcctc ggatgccttc gggctcaccc tcgcaggcta
     1801 tccgagcact gtgacatcga aaccggtcgg accgggtgtg atcacctgcg cgtttgacat
     1861 tatgcaggta tatacctgca taatggtcga gtggccgacg atccgatgga caaggtgttc
     1921 aaggcgctcg ccgacccggg ccgccggcta ctgctggatc ggttgcgcca ggacaacggc
     1981 caaacactcg gccagctgtg cgagtacctc gaaatggcgc gccagtcggt gagccagcat
     2041 ctggccgtgc tcgagtcggc caatctcatc agcagcgtgc ggcgtggacg gctcaagttg
     2101 cactacttga atcccgttcc gctgcaagag attgcgtacc gctggattca accgttcgaa
     2161 acttatcggc ttcacgccct gcacgaagtg aagctccaag cagaggaggc acacatgact
     2221 gggacaaccg acaagccgac gttcgtatac gtcacataca tccacagcag cccggagaag
     2281 gtatgggaag cgctcaccag cgccgagctg accgccgcgt actggggaca cagcaatgtg
     2341 tcggactggc agaaggggtc acggtgggag cacatccgca ccgacggatc gggagtcgtc
     2401 gatgtcgcgg gcgaggtgat cgaagcgaca ccgccgcgtc gactcgtcat cactttcggc
     2461 ggcgccgacg aggccggtca gccgaccgtc gtgacgttcg acgtggaacc gcaccacgac
     2521 attgtccgcg tcaccgtgac ccacgagggg ctggacgatc cgtcggacta ccgtgccgtt
     2581 tcgacgggct ggccggccgt gctatcgaac ctgaagtcgc tgttggagac cgggcgcgta
     2641 ctgccgcagg cgccatggga aatgcatgcc gacctacgcg cccaacagat gtcgggctag
     2701 cgggtagcgt ggcgacatgc cgcatccgat catgttccgc gacgacgatc cggatctcgc
     2761 ggaggtgcgc aagatcgcac tgagctatcc cgacgcgttc gaaaaggtct cccacggcag
     2821 gcccgcgttc ttcgtctcga agatgttcgc catgtacggc ggtagcgcca agccggcgac
     2881 caagggtgac tacatctcgt acccgcattc gatcatggtc aaggtcgatg agagcgaccg
     2941 cagagcgctc gggcaggaca ctcggttttt ctacccggcg tatctgggcc cggcgggatg
     3001 gctcgggctg gatctcaccg cgacgaaggc gatcgactgg ggcgaggtgg gtgagctgat
     3061 cgacgcgtcc ttccggatgg tcgcaccgaa gaagctcatc aagcagctcg accacgcctg
     3121 aaccgcgccg ggccgacatg gttctatcga ctccatgagc ttcgccagcc cctatcccga
     3181 cgtcgagatc ccgaacgtca gcgtttatga atacctgttc ggcaacctcg acgatgcaga
     3241 cgccgacaac gtcgcgctgg tggacacgaa atcgggtaat gcgatcagct accgcgacac
     3301 cgtcggcaac ataaattcat tcgccggtgc actggccgat cgcggtatcg cggtcggtga
     3361 tgtggtcggt ctgctggcgc cgaacagcgc cgcgttcgcg atcgccttcc acggcatcct
     3421 gcgcgccggt gccacggcca ccaccatcaa cgtgttgttc accgcgaagg acatcgtcaa
     3481 gcagctgacc gattccaagg ccacgatgct cgtcacggtc accccgctgc tctcccaggc
     3541 cagggaggcc gccgccgcgg tgggcatatc cgacgaacaa ctggtggtgc tcgacggcgc
     3601 cgacggccac ccgagtgcgg cggatcttct cggcgcaggc gcgacaccgc cggaggtcag
     3661 cttcgacccc gagacgcacc tggccgtact gccctacagc tcgggcacga ccggcaatcc
     3721 caagggcgtg atgctcacgc atcgcaacct cgtcgccaac atcgcccaga tccgtgcggc
     3781 ccaggacgtc gaggccgacg acgccgtcat cgccttcttg cccttcttcc acatctacgg
     3841 aatgacggtg ctgctcaacg ccgtcctgcg ggcgcgagcg cggctggtca tgatgcccag
     3901 cttcgacctc gaggagttcc tcgcgaacat ccagaaccac agggtcaccg tggctttcat
     3961 cgcgccgccg gtggcggtgg cgctggccaa gcatccgctc gtggacaaat acaacgtgtc
     4021 gacgctgcgc gcggtgctgt ccggcgcggc accgttggac gaggacctgg gccacgcagt
     4081 ggccgaccgc atcggatgcc cggtggtcca gggctatggc atgaccgaac tgagtcccgt
     4141 cagccactgc acgccgttcg acggcggcat gcggctcgtc gggtcagccg ctcccatcgg
     4201 cgctgccgga tggacggcgg ccaattcctc gtccaggctc atcgagcccg atagcggcga
     4261 cgaaatcgat gttcccgctg cgggtctcag tgagaccggc gagttgtggt tcaggggacc
     4321 gaatgtgatg gccggctatc tcggcaacga ggaagcaacc agggaaacga tcgacgacga
     4381 cggctggctg cgcaccggag acctggcccg ggtcgactcg tcgggatgtg tgtacatcgt
     4441 cgaccggctc aaggaactca tcaagtacaa gggttaccag gtgccgcccg ccgaactgga
     4501 ggcggtgctg ctgacccacc cggcgatcgc cgacgccgca gtgatcgggg tgcgcgacgc
     4561 ggattccggc gaagaggtgc caaaagcttt cgtggtcaag caatccgggg ccgagctgac
     4621 cgaggcagac gtgatggact tcgtggccgg tgaggttgcg ccctacaaga aagtgcgaca
     4681 ggtggcattc atcgatgcga tcccgaagtc gtcggcgggc aagatcctgc gcaaggacct
     4741 gcgcctggac tcaactgcgc agtgactccc gcagcgactg ggcggcccgc gcctgccggt
     4801 agccgagcac ggctccgatg gcgggtatca gaatcaaacc cgcgacgccg gccagcggat
     4861 cggccaccgt atcggttgtc ccggtgccgt attcgccgac ggtgaggtgg gccgggcgga
     4921 tgcgctcgac cggtggcatg ggtggcggca gcggcggaaa gttgatctcg atggccgcgg
     4981 gcacccctgg cgcggcagat accgccgcgg gcgagaccat cgcgtcgtcc gtcagcaccg
     5041 cctcggctgc tgggccgggc atcggaacgt gggttcccgg tgtgcggccg ttgccgacga
     5101 caaccttcgg tccgcggaac ggtgcaaggg gcgggcccga gtaaccggac ccggcccttg
     5161 gcgcggcggc cgaaacgttg tcgcctgggg gctgctcgac taccgcagca gaccgcagtg
     5221 cggtcaggct ttcggtgccc aacccggtca ccacggcagg gtcctcgaga tcgctgccgc
     5281 ccacggatag attcatcgac ggcggagtga gatcggtgtc ggcggtgccg ccgacctgca
     5341 gagcgtcctc caccgcattc gccacgccgg gctcaacgtc gccgaggggg ccgcctgaac
     5401 cgttcttccg gctgtcgttc ttggcatggg cgcccgaacc gtgcttgtcg gcgaccgcgt
     5461 gcgcggggtt cggaccggcg atcagcagag acgctgcgag cacaccggct cccgccgcga
     5521 agcgaacagt cgagtgaccc acgatccctt cctgacttac tgggctcgcg ggcccaggtt
     5581 gcgcccatgc attgttacac aaacctttgc ccggcaaacg ctatttcatg atcgcggccg
     5641 ggtcggcggc gatggcgtcg gcggcttcct ggacgagttc gatcgcgatg cgggcccatt
     5701 gcggagtcgc gccggccagg ccgcataccg g
//
LOCUS       DASYLX010000460         6662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3312738,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000460 DASYLX010000000
VERSION     DASYLX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312738"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGP27_11200"
     CDS             <1..439
                     /locus_tag="VGP27_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851907.1"
                     /translation="LDQWQKVIDVNLTGTFLGMRAAVQPMIDAGGGSIINVSSIEGLR
                     GAPFVHPYVASKWAVRGLAKSAALELAPKNIRVNSVHPGFVRTPMTAHLPEDMVTIPL
                     GRPAEPEEVATFIVFLASDESSYSTGSEFIMDGGLVTAVPSKT"
     gene            complement(448..756)
                     /locus_tag="VGP27_11205"
     CDS             complement(448..756)
                     /locus_tag="VGP27_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEV7851908.1"
                     /translation="MTQPEPARPEPGPDADIDDIQTDIEQTRKELGETVEALSAKMDV
                     KQRTKDKAAETKEAIVEKAHTVQHATIDDGRAKVTVPMAAVAVVAAATLVWFLRRRRR
                     "
     gene            complement(753..1154)
                     /locus_tag="VGP27_11210"
     CDS             complement(753..1154)
                     /locus_tag="VGP27_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV7851909.1"
                     /translation="MSVESKPAADASIGELMSQLATQTSRLVRDEMRLATKELQQSAK
                     HAGVGAGLFSVAGLLSILGLMTLIAASVAALALVLPVWAAAVIVAVVLFLAAGIAALV
                     GRKQAAEIAPPPKEIVESLKADIKEVKDARS"
     gene            complement(1193..1900)
                     /locus_tag="VGP27_11215"
     CDS             complement(1193..1900)
                     /locus_tag="VGP27_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEV7851910.1"
                     /translation="MNDPAGLVATHLRIAEIVQELYGRADVDSDTVIAELAEHAAVEI
                     PGAQYAGVTVTRNGRQIDTPAATHKWPILLDEIQQRHREGPCLTAAWEEKTIHVKDLG
                     TDERFPLYARDVLAETPIRSVMSFQMWIAGETMGALNVYAESPNVFGTETREIGLVFA
                     AHSSVAWNAARRDEQFKKALASRDVIGQAKGMLMERYGVSAIQAFDLLRKLSQDSNTP
                     LIQIAADLVEKQQSSIE"
     gene            1969..3060
                     /locus_tag="VGP27_11220"
     CDS             1969..3060
                     /locus_tag="VGP27_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851911.1"
                     /translation="MYGQRRHRHGVITRSAIGFGAAEAFTIIAIATVLITRLYLQLAG
                     YPQVGGGDLHIAHALYGGALMMLALLIGWLMLGFGARAACVILGGIGFGLFLDEVGKF
                     VTKNNDYFYGPAAEIMYILVVVILVGARIVRDFRPLSARECLASAAMVAADGVARGLA
                     ARRREIGLGLAERAGAGGADVDEVAAVRALLLSADPASDRLHALQERAIRLVPDFLRS
                     PRWVPILGWLIVVGAFFSVVFGALGVALGGWYYEDDRMTLELDGMSISSVILLVSAGL
                     TLGMALPAMIVRRRSDSVWPLVWLRNAALVFTLLNALVDFATEGFGALLNMSIGLFTL
                     AIMSYQLDLAARAVVPPEEWAKAPSGSAP"
     gene            complement(3032..3529)
                     /locus_tag="VGP27_11225"
     CDS             complement(3032..3529)
                     /locus_tag="VGP27_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEV7851912.1"
                     /translation="MRLTDLSALQLTYREVGATAGPLPEGYHHVRKSAVIGRGRPRFD
                     EAAAKVMRWGMLRGAGVRVDASSEVAAVGSEVLVGLGPLRAPCRVVYVVDEPDRRGFA
                     YGTLPGHPETGEEMFGVRYDSATDEVHAEVVAFSRHGMWWSRLGSPVTALAQRVITAR
                     YLTAL"
     gene            3568..4443
                     /locus_tag="VGP27_11230"
     CDS             3568..4443
                     /locus_tag="VGP27_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7851913.1"
                     /translation="MTTSSVAQAAGTFALGGDLTVNRLGFGAMRITGKGVWGPPDDHD
                     EAIRVLRRAVDLGVNFIDTADSYGPYVSEELIHEALSPYPDELVIATKAGLLRTGPDV
                     WIPLGFPAYLRQELEMSLRRLGVDRIDLFQLHRIDDKFPLEDQIGELAALQQEGKIRH
                     IGLSEVDVTQLEAAQKIAPIVSVQNMYNLTARGAEPVLEACEAQGLGFIPWFPLAAGP
                     LAAPDGPLQRIAADHHATASQLALAWLLKRSPVMLPIPGTSKVSHLEQNVAAAEITLS
                     DEEFETLSKAGAESG"
     gene            4446..5060
                     /locus_tag="VGP27_11235"
     CDS             4446..5060
                     /locus_tag="VGP27_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851914.1"
                     /translation="MRLAFTGHIAGAGTGSGLRVVVGAWDESPFGAFTDVMLQCADDE
                     RVLLVPDDSVAEFVASTYRFDRVEVGAVSAGFCAGQLVVTTENFDARFRIGGPARVDH
                     LLRLVPRRLATSPRWLRVIDPVAARVLPGVHTYGTAGNGRVEYYGVRRSRRITAIDGH
                     YRGSPFGGLAPLRPPVSFGFSSAPSAPQIVSVTTTIDESREAIR"
     gene            5065..5688
                     /locus_tag="VGP27_11240"
     CDS             5065..5688
                     /locus_tag="VGP27_11240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7851915.1"
                     /translation="MATDTNAAHAGGGFNPPEPTTRGGPDYGRFIEAVRTLGDHTRAV
                     DAPDHVITEAADMIEAVSRLLAPYDADEWTTPSGRRMDLPNRGNIMQVPADLKTVGDE
                     VDGTVRFRRFHLGRNGAVHGGALALMWDSVLGFAAFRLTRSLKQRTAYLHVNYRKIVP
                     IEMDLQVDARVDRSEGRKIFVTGRLLDHDEVLCDAEALFVTLNPGQP"
     gene            5733..6158
                     /locus_tag="VGP27_11245"
     CDS             5733..6158
                     /locus_tag="VGP27_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02611 family protein"
                     /protein_id="HEV7851916.1"
                     /translation="MGSRWSATKRHWARWRDRLRERPKVEFGYRIVVGVIGLAVLGLG
                     IVAIPYPGPGWAIVFIGLGILATEFDWARRLLAYAKERYDKVMDWFHRQGKFVQILGG
                     LVTALIVAATLWLLGALDWSAEFVGIEHPWLNSPIGVGD"
     gene            6247..>6662
                     /gene="thrS"
                     /locus_tag="VGP27_11250"
     CDS             6247..>6662
                     /gene="thrS"
                     /locus_tag="VGP27_11250"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894690.1"
                     /note="catalyzes the formation of threonyl-tRNA(Thr) from
                     threonine and tRNA(Thr); catalyzes a two-step reaction,
                     first charging a threonine molecule by linking its
                     carboxyl group to the alpha-phosphate of ATP, followed by
                     transfer of the aminoacyl-adenylate to its tRNA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV7851917.1"
                     /translation="MTAAPSPAPAAPIRVAAGTTAAAAVRDAGLPSRGTSDAVVVVRD
                     ADGRLRDLSWAPETDVEVTPVAADTDDGRSVIRHSAAHVLAQAVQELFPDAKLGIGPP
                     ITDGFYYDFDVAQAFTPEDLEALEKRMRQIVKDGQL"
BASE COUNT         1034 a         2272 c         2199 g         1157 t
ORIGIN      
        1 cctggaccag tggcagaagg tcatcgacgt caatctgacc ggcacgttcc tcggcatgcg
       61 cgcggcggtc cagcccatga tcgatgcggg cggcggttcc atcatcaacg tgtcgtcgat
      121 cgagggcctc cgcggcgccc cattcgttca cccctacgtg gcgtccaagt gggcggtgcg
      181 cggactggcc aagtcggccg cactcgagtt ggcgcccaag aacatccggg tcaactcggt
      241 gcatcccggg ttcgtccgca caccgatgac cgcgcacctg cccgaggaca tggtgaccat
      301 tccgctcggt cgtcccgccg aaccggaaga ggtggccacc ttcatcgtgt tcctggccag
      361 cgacgagtcg tcatactcga ccggcagcga attcatcatg gacggcggcc tggtgaccgc
      421 cgtcccgagc aagacctagc agccacctca gcggcggcgc cgccgtagaa accacaccag
      481 cgtcgcggcg gcgacgacgg ccaccgcggc catcggcacc gtgaccttcg cgcgcccgtc
      541 gtcgatggtc gcgtgctgaa ccgtgtgcgc tttttcgacg atggcctctt tggtctcggc
      601 ggccttgtcc ttggttcgct gcttgacgtc catcttggcc gacaaggcct cgacggtttc
      661 gccgagttcc ttgcgggtct gctcgatgtc ggtctggatg tcgtcgatgt ccgcgtcggg
      721 accgggctct ggacgagcgg gttcgggttg agtcatgagc gcgcgtcctt cacttccttg
      781 atgtccgcct tcaggctttc gacgatttcc ttcggcggcg gcgcgatctc cgctgcctgc
      841 ttgcggccca caagcgccgc gatgcccgcc gccaggaaaa ggaccaccgc aacgatcacg
      901 gcggcggccc acaccggcag gacgagcgcc agtgccgcca cgcttgcggc tatcaacgtc
      961 atcaggccca ggatggacag cagacccgcc acgctgaaca acccggcgcc gacgccggcg
     1021 tgcttcgcgg actgctgcag ctccttggtc gccaaccgca tctcatcgcg tacgaggcgc
     1081 gacgtctgcg tcgccaactg gctcatcaat tcgccgatcg aggcatcggc agccggtttc
     1141 gattccacac tcatgcgggg tggatttccc cggccacacg ggacgaaaca cgctattcga
     1201 tcgaggactg ctgcttttcc acgaggtcgg cggcgatctg gatcaacggc gtgttcgagt
     1261 cctgcgacag cttgcgcagc aggtcgaagg cttgaattgc gctgacgccg taacgctcca
     1321 tcagcatgcc cttggcctgc ccgatgacat cgcggctcgc aagcgccttc ttgaattgct
     1381 cgtcgcggcg ggccgcattc cacgcgaccg aggagtgagc cgcgaagacg aggccgattt
     1441 cacgtgtttc ggtgccgaag acattcggtg actcggcgta gacattgagc gcacccatcg
     1501 tctcgcccgc gatccacatc tggaaggaca tcaccgagcg aattggagtc tccgccagta
     1561 catcccgtgc atacagcgga aaccgttcgt cagtcccgag atccttgacg tggatggtct
     1621 tctcttccca tgccgccgtc aggcacggtc cctcgcgatg gcgctgctgg atctcgtcga
     1681 gcagtatcgg ccacttgtgc gtcgcggcgg gcgtgtcgat ctgcctgccg tttcgggtga
     1741 ccgtcacccc ggcgtattgc gcgccgggta tctcgactgc ggcgtgctcg gccagctcgg
     1801 cgatgacggt atcggaatcg acgtccgcgc ggccgtagag ctcttgcacg atctcggcga
     1861 tgcgcaggtg ggtcgctacc aacccggctg gatcgttcac cgctgtcatc cgatcattct
     1921 gctccgataa gttgctttag gctaacgcgg gcgtaaggcg gagagcgtat gtatggacaa
     1981 cggcgtcacc gccacggtgt tatcacgcgc agcgcgatag gtttcggtgc cgcggaagcg
     2041 ttcacgatca tcgcgatagc gaccgttctc atcacccggt tgtatctcca gctggcgggc
     2101 tatccgcagg tcggcggggg tgacctgcac atcgcccacg cgctctacgg cggcgctttg
     2161 atgatgcttg ccttactgat cggctggctg atgctcggct tcggtgcccg ggcggcatgc
     2221 gtgatactcg gcggcatcgg gttcgggttg ttcctcgacg aagtcggcaa gttcgtcacc
     2281 aagaacaacg actacttcta cggacccgcc gccgagatca tgtacatcct ggtggtcgtc
     2341 attctggtcg gcgcccggat cgtgcgcgac ttccggccgc tcagcgccag ggagtgcctg
     2401 gcgtcggccg cgatggtcgc agccgacggc gtcgcgcgcg ggctggccgc ccggcgccgt
     2461 gagatcgggt tgggcctcgc cgaacgggcc ggcgccgggg gagccgacgt cgacgaggtg
     2521 gccgcggtgc gggcgctgct tctgtctgcc gaccccgcgt ccgatcgcct gcatgccttg
     2581 caggagcgtg cgattcggtt ggtccccgac tttctgcgca gcccccggtg ggtgccgatc
     2641 ctgggctggc tgatagtggt cggcgcgttc ttcagtgtcg tcttcggcgc gctgggcgtc
     2701 gctctcggcg gctggtacta cgaggacgat cgaatgaccc tcgaactgga cgggatgagc
     2761 atctcgtcgg tcatcctgct cgtcagcgcg ggtctgacgc tcggtatggc gctgcccgcg
     2821 atgatcgtgc ggcgccgctc tgacagcgtg tggccgttgg tctggctgcg caacgcggcg
     2881 ctcgtgttca cgctgctcaa cgcgttggtc gacttcgcca ccgaaggctt cggcgcgttg
     2941 ctcaacatgt cgatcggcct gttcacgctg gcgatcatgt cctatcagct cgacctcgcc
     3001 gcgcgggccg tcgtgcctcc ggaggagtgg gctaaagcgc cgtcaggtag cgcgccgtga
     3061 tgacgcgttg cgccagcgcc gtgacgggcg agccgagcct gctccaccac atgccgtgcc
     3121 gggagaacgc aacgacctcg gcgtgcacct cgtcggtcgc ggagtcgtag cggacgccga
     3181 acatctcctc gccggtttcg ggatgacccg gaagggtgcc gtaggcgaac ccgcggcggt
     3241 ccggctcgtc gaccacgtac acgacccggc agggtgcccg cagtggcccg agcccgacca
     3301 gtacctcgga cccgacggcc gccacctcgc tgctcgcgtc gacgcgcacg cccgctccgc
     3361 gcagcattcc ccaccgcatg accttcgccg ccgcctcgtc gaatcgcgga cgcccgcggc
     3421 cgatcacggc cgacttgcgc acatggtgat agccctcggg caacgggccc gccgtcgcac
     3481 ccacctcgcg gtaggtcagt tgcagtgccg acaagtcggt caatctcacc ccgacaccat
     3541 gtcatcaccc ggcgtacggt ctgaaaggtg accacttcat ccgtcgccca agccgcagga
     3601 accttcgcgc tcggaggcga tctcaccgtc aatcgactcg gattcggtgc catgcggatc
     3661 accggcaagg gcgtgtgggg gccgccggac gatcacgacg aagccatccg cgtgttgcgg
     3721 cgcgcggtgg acctcggcgt caacttcatc gacaccgccg actcctacgg tccgtacgtt
     3781 tccgaggaac tgatccacga ggcactctcg ccctatcccg acgaactcgt catcgccacc
     3841 aaggccggac tgctgcgcac cggcccggat gtctggatac cgctggggtt tcccgcctat
     3901 ctgcgccagg agctcgaaat gagcctgcga cggctgggcg tcgaccgcat cgacctcttt
     3961 caattgcacc gcatcgacga caagttcccg ctcgaggatc agataggtga gctcgccgcg
     4021 ctgcagcagg agggcaagat ccgccacatc ggcctgtcgg aggtcgacgt cacgcagttg
     4081 gaggcggccc agaagatcgc cccgatcgtg tcggtgcaga acatgtacaa cctcaccgcc
     4141 cgcggggccg agccggtgct cgaggcgtgt gaggcccaag ggctcggctt cattccgtgg
     4201 ttcccgctcg ccgcaggacc gctcgcggca cccgatggcc cgctgcagcg catcgcggcg
     4261 gaccaccacg ccaccgcgtc gcagctcgcc ctggcgtggc tgctgaagcg ttcgccggtg
     4321 atgctgccca ttccgggcac gtcgaaggtg agccatctgg agcagaacgt ggccgcggcc
     4381 gagatcacgc tttccgacga ggaattcgag acgttgagca aggccggcgc ggaatccggc
     4441 tagcggtgcg gctggccttc accggacaca tcgcaggcgc gggcacgggt tcggggctgc
     4501 gcgtcgtcgt cggcgcctgg gacgagtcgc cgttcggcgc cttcaccgac gtcatgctgc
     4561 agtgcgccga cgacgaacgc gtgctgctgg ttcccgatga cagcgtcgcc gagttcgtgg
     4621 catcgaccta ccgattcgac cgcgtcgaag tgggcgcggt gtcggcgggt ttctgcgcag
     4681 ggcagctcgt ggtaaccacc gagaacttcg acgcgcgctt ccgtatcggc ggacccgcac
     4741 gcgtcgacca tctgctgcgg ctggtgccgc ggcgattggc gacctcgccg cgctggttac
     4801 gggtgatcga cccggtcgcg gcgcgcgtgc tgccgggggt gcacacctac gggaccgcgg
     4861 gcaacggccg agtcgagtac tacggtgtgc ggcggtcgcg gcgcatcacc gcgatcgacg
     4921 gccactaccg aggttcgccc ttcggcgggc tggcaccgct gcggccaccg gtgagtttcg
     4981 gcttctcctc ggcaccgtcc gcgccgcaga tcgtgtcggt gaccacgacg atcgacgaat
     5041 cccgggaggc aatacggtga gcgggtggcc accgacacca atgctgcgca cgccggtggc
     5101 ggattcaacc cgcccgaacc caccacccga ggcggaccgg actacggccg gttcatcgag
     5161 gccgtccgca cgctggggga ccatacccgc gcggtggatg cgccagacca cgtgatcacc
     5221 gaggcggccg acatgatcga ggcggtgtcc cgcctgctgg cgccctacga cgccgacgaa
     5281 tggacaacac cgtcggggcg gcgcatggac ctgcccaacc gggggaacat catgcaggtg
     5341 cccgcagacc tgaagaccgt cggggacgag gtggacggca cggttcgctt tcgtcgcttc
     5401 cacctcggcc gcaacggagc ggtgcacggc ggggcgctcg cgctgatgtg ggactcggtc
     5461 ctcggcttcg ccgcgttccg gctcacccgc agcctcaaac aacggaccgc ataccttcac
     5521 gtgaactatc gcaagatcgt cccgatcgag atggatttgc aagtcgacgc cagggtcgac
     5581 cgcagcgagg gccgcaagat cttcgtcacg gggcggctcc tcgaccacga tgaggtgctc
     5641 tgcgatgccg aggcattgtt cgtgacactc aaccctgggc agccgtgaca aacgacgaaa
     5701 ccggcaccga gtcgcgtagc cgcggcggcg acatcggatc gcggtggtcg gccaccaaac
     5761 gccactgggc gcgctggcgc gaccggctgc gggagcgccc caaggtcgag ttcggctacc
     5821 gcatcgtggt cggagtgatc gggctcgcgg tcctcggcct cggcatcgtc gccatcccgt
     5881 atcccggacc tgggtgggcc atcgttttca tcgggctcgg catcctggcc accgaattcg
     5941 actgggcgcg gcggctgctg gcctacgcca aggagcgcta cgacaaggtc atggactggt
     6001 ttcaccggca gggcaagttc gtacagatcc tcggcggctt ggtcaccgcg ttgatcgtgg
     6061 ctgcgacgct gtggctgctc ggggcgctcg actggtccgc cgagttcgtc ggcatcgaac
     6121 atccgtggct gaacagcccg atcggggtcg gggactgacc tcctgcaccg atagcatggg
     6181 tcgcgttcgt aacacccaca cgatccccgc gcacgcgagg agcacgactt ctacagagag
     6241 tgaccaatga ccgccgcccc cagccccgcc cccgcagccc cgatccgggt agctgcaggg
     6301 acgaccgctg ccgcggcggt tcgcgacgcg ggacttccga gccggggtac ctccgacgcc
     6361 gtcgtcgtgg tccgtgatgc cgacggccgg ctgcgcgacc tgagctgggc accggaaacc
     6421 gatgtcgagg tgacgccggt cgccgccgac accgacgacg gccgcagcgt gattcgtcac
     6481 tcggcggccc acgtgctggc ccaggcggtg caggagctgt tccccgatgc gaagctcggc
     6541 atcgggccgc cgatcaccga cgggttctac tacgacttcg acgtcgccca ggccttcaca
     6601 cccgaggacc tggaggcgct ggagaagcgc atgcgccaga tcgtcaagga cggccagctg
     6661 tt
//
LOCUS       DASYLX010000461         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_680112,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000461 DASYLX010000000
VERSION     DASYLX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680112"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGP27_11255"
     CDS             <1..312
                     /locus_tag="VGP27_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851918.1"
                     /translation="SLTMHIACQYGPQGIRCNAVAPGVTLTPMVAQRLEDERFKKINT
                     EMTPYPRLGSIDDIASTVAFLCSDGAAFINGQTIAVDGGWSSTKYLSDFALNSEWVAR
                     R"
     gene            389..>1645
                     /locus_tag="VGP27_11260"
     CDS             389..>1645
                     /locus_tag="VGP27_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7851919.1"
                     /translation="MNLFSLLDQAAARFGDRGAVYRGEDQLHTWAGLRHRALRLAGSL
                     RGVGPGARVAVASENCPEIIELMFGVWAAECVYLPINYKLHPREMQQILDDAAAAVVF
                     ASPKIAADLAPVTSVPIEVIDDGGYDARFGAEPVPTPCTDPAELAWLFYTSGTTGRSK
                     GAMLSHRNLMAMTVSHLADFDSPDEDCGLIHGAPMSHGSGLYIPPYVLRGARQVIPAS
                     GAFEPDEFLDLCEHHPGCSAFLAPTMVARLVQTGRSRPGNLRTVVYGGGPMYVDSLKK
                     AMTAFGPIFTQLYGQGEAPMTITGLRRADHVDADDAVLGSVGYARSGVEVAVQRDDGS
                     HAEIGEIGEIVCRGDVVMSGYWRNPAATAATLQNGWLHTGDMGSFDERGYLTLRDRSK
                     DVVISGGSNIYPREVEEAILEHPAVVE"
BASE COUNT          277 a          545 c          557 g          266 t
ORIGIN      
        1 tcgctgacca tgcacatcgc ctgccagtac gggccgcagg gaatccgttg caacgcagtg
       61 gcgccgggcg tgacgctgac gccgatggtc gctcagcgcc tcgaggacga gcggttcaag
      121 aagatcaaca cggagatgac gccctacccc cggctgggca gcatcgacga catcgccagc
      181 accgtcgcct tcctgtgttc cgatggcgcc gccttcatca atggccagac gatcgctgtc
      241 gacggcggct ggagttcgac gaagtacctg tccgacttcg cgctgaactc ggagtgggtg
      301 gcccggcgat gagtgcttgc gcgaagtggg ggcactcccc aagcaagctg ggggcccacc
      361 cgcccgaagg gcagggggac agaacccggt gaacctgttc agtctgctcg accaggccgc
      421 tgcccgtttc ggcgaccgtg gtgcggtgta tcgcggtgag gaccaactgc acacctgggc
      481 cgggcttcgg catcgggcgc tgcgactggc gggctcgctt cgcggggtgg ggcctggggc
      541 gcgcgtcgcg gtcgccagcg agaactgccc cgagatcatc gagctcatgt tcggcgtgtg
      601 ggcagccgag tgtgtgtacc tgccgatcaa ttacaagctg cacccacggg agatgcagca
      661 gatcctcgac gatgccgccg ccgcagtggt tttcgcctca ccgaagatcg ccgccgacct
      721 cgcacccgtc accagcgtgc cgatcgaggt catcgacgac ggtgggtacg acgcccggtt
      781 cggcgccgaa ccggtgccga cgccgtgcac cgacccggcg gagctggcgt ggctgttcta
      841 caccagcgga acgaccggcc ggtccaaggg tgcgatgctg tcgcatcgca acctgatggc
      901 gatgacggtc agccacctcg ccgacttcga ttcacccgat gaggattgcg gcctgatcca
      961 cggtgcaccg atgtcgcatg gatcgggcct ttacattcct ccgtacgtgt tgcgcggagc
     1021 acggcaggtg attcccgcgt cgggagcgtt cgagcccgac gagttcctcg atctgtgtga
     1081 gcaccaccct ggctgcagcg ccttcctggc cccgacgatg gttgcgcgac tggtgcagac
     1141 cggccggtca cgtcccggca acctgcgcac ggtggtctac ggcggcggcc cgatgtatgt
     1201 ggacagcctc aagaaggcga tgaccgcgtt cggaccgatc ttcacgcagc tttacggaca
     1261 gggtgaggcg ccgatgacga tcaccggtct gcgccgcgcc gaccacgtcg acgccgacga
     1321 cgccgtcctc gggtcggtcg gctatgcccg ctccggcgtg gaagtagcgg tgcagcgcga
     1381 cgacggcagc cacgccgaaa tcggggagat cggtgaaatc gtctgccgcg gcgacgtcgt
     1441 gatgtccggc tactggagga accccgccgc caccgccgcc accctgcaga acggctggct
     1501 gcataccggg gacatgggct cgttcgacga gcgcggctac ctcaccctgc gcgaccgttc
     1561 gaaagacgtg gtgatcagcg ggggcagcaa catctaccca cgcgaagtcg aagaggcgat
     1621 cctcgaacat cccgccgtcg tggag
//
LOCUS       DASYLX010000462         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_899722,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000462 DASYLX010000000
VERSION     DASYLX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899722"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VGP27_11265"
     CDS             <1..132
                     /locus_tag="VGP27_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV7851920.1"
                     /translation="VDTLRTVHDEVDGEDPSTADILHQLIDGLEKLAWLIKSENRKV"
     gene            168..620
                     /locus_tag="VGP27_11270"
     CDS             168..620
                     /locus_tag="VGP27_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7851921.1"
                     /translation="MTVTRDIHASRRQVWDVLADGWTYSQWVVGNSRMRAVDPNWPAP
                     GSRILHSVGVWPFLINDETVVEKSVPLEELVLRAKGRPLGEARVILRLSDIPDGCRVE
                     MEEFPVSGLGAVLPSRLSDMAAWPRNRETLRRLAAIAERRKPSEVKDR"
     gene            complement(607..1899)
                     /locus_tag="VGP27_11275"
     CDS             complement(607..1899)
                     /locus_tag="VGP27_11275"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7851922.1"
                     /translation="MTRRARSVCMPELALKTSVPARTQHYADSVASRFRVLKIATWIA
                     ATISVAFAIFQLLLGGPAWWVGLLNLVFGAAFLTVPMLYRFGELIAPLTFFVFAYLSV
                     GYICYTIGTGSGLQFYFLVAATLVVLVFGIERIVLASMIAAFGAAGVIALELLVPNDR
                     HLGPPWTLTVGFVSSVIGSAVMVMATVWFALREIDRAEKAMEAEYQRSESLLANILPA
                     SIASRLKDPTRNIIADKYDDASILFADIAGYTKRASDTAPTELVRFLDTLYTDLDALV
                     DRHGLEKIKTSGDSYMVVSGVPQPRPDHVQAIACLALDMAEAVADLKDAQGRAVPLRI
                     GLATGPVVAGVVGARKFFYDVWGDAVNVASRMESTDVEGRIQLPQSMYERLKNDYVLE
                     ERGVVDVKGKGPMHTWYLVGRRNGHTSHAAELQSVSGP"
     gene            2195..2968
                     /locus_tag="VGP27_11280"
     CDS             2195..2968
                     /locus_tag="VGP27_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851923.1"
                     /translation="MVGGPGVVGPAVAHADLFGFGFDFCGGDDKSDLHHPRPGSEVSA
                     QTTRISAVADAAAPTAKVGSLPENVDAPASAELRSAAGVPENVGVAESAGGGAGGVPR
                     TNITGRAANLPRVSSAPVTRSIVIRGVPRAVAPAFVPPTLRQAPVTAGLAAPAPVSPE
                     PEGQPAPAAPPAPSPTAPQTKDPLASRHSGIARISDSYRAGYADHLRAATVSDLFAEA
                     LPGVAGIAGFTLIGAFAGYRQARALKRALLAPVPTSVLL"
     gene            3087..>3754
                     /locus_tag="VGP27_11285"
     CDS             3087..>3754
                     /locus_tag="VGP27_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851924.1"
                     /translation="MACGALLVGPSLVGTGVAKADLLGVGGGGGGIDVLGVDVLGGRA
                     KGSGASGSAARLSAVSTAPSTRSVVIRAKEPAAQPDPTAVPAAFTAPAAEAPAVALSA
                     PVVDAIPAAPPPAAPPMAATGPLSIQPPAAPAVLSPAPLAAPVIPSTSAPGPGRPLGP
                     ADIHPRVTKVPDSFRVGYPEYLRSATNTDLLVAAVPGVAGIAGFTLIGAFAGYRQAKA
                     VQMA"
BASE COUNT          625 a         1247 c         1258 g          624 t
ORIGIN      
        1 gtcgacacgc tgcgcacggt gcacgacgag gtcgacggcg aggatccaag caccgccgac
       61 atcctgcatc agctcatcga cggcctggag aagctggcct ggctgatcaa gtcggagaac
      121 cgcaaggttt aggcgtttgt cgccgacgca gcgggtatcc cacccgcgtg acagtgacac
      181 gagacatcca cgcgtcgcgc cggcaggtgt gggacgtttt ggccgacggc tggacgtact
      241 cccagtgggt cgtcggcaac agccggatgc gcgcggtaga tccgaactgg cccgcgcccg
      301 gctcgaggat cttgcattca gtcggcgtgt ggccgttttt gatcaacgat gagacggtcg
      361 tcgagaaaag cgttccgctc gaggaactgg ttctgcgcgc caagggccgc ccacttggag
      421 aggcccgagt catcctgcgg ctcagcgaca tacccgacgg gtgccgggtc gagatggagg
      481 agtttccggt cagcgggctc ggggccgtgt tgccaagccg gctgtcggac atggctgcct
      541 ggccacgcaa ccgcgagacg ctgcggcggc tcgccgcaat agccgaacgg cggaaaccct
      601 cagaagtcaa ggaccgctga ccgactgcaa ttcggccgca tgcgaggtgt gcccgttgcg
      661 tctgccgacc agataccagg tgtgcatcgg gcccttgccc ttgacgtcga cgactccgcg
      721 ttcctcgagc acgtagtcgt tcttgagtcg ttcatacatc gactgcggaa gttgaattcg
      781 gccttcgacg tccgttgact ccatgcgcga tgcgacgttc accgcgtcac cccacacgtc
      841 atagaagaac ttgcgcgcgc cgacaacccc cgccacgacc gggccggtgg ccaacccgat
      901 gcgcagcggc accgcccggc cctgcgcgtc cttcaggtcg gcgaccgctt ctgccatgtc
      961 gagggcaagg cacgcaattg cctggacgtg atctggccgc ggttgcggca ctccgctgac
     1021 caccatgtac gagtcgccgc tggttttgat cttctccaga ccgtgcctgt cgacgagggc
     1081 gtcgaggtcg gtgtacagcg tgtccaagaa ccgcaccagc tcggtcggcg cggtatcgct
     1141 ggcccgcttg gtgtatccgg cgatatcggc gaacaggatc gaggcgtcgt cgtatttgtc
     1201 ggcgatgatg ttgcgcgtcg ggtccttgag cctgctcgcg atggacgccg gcaggatgtt
     1261 ggccagcaac gactctgaac gctggtactc ggcttccatc gccttctcgg cgcggtcgat
     1321 ctcacgcaac gcgaaccaca ccgtcgccat caccatgacc gccgatccga tgacggagct
     1381 gacgaaaccg accgtgagcg tccatggcgg acccaaatgc ctgtcgttgg gaacgaggag
     1441 ctcgagggcg atgacgcctg ccgcgccgaa ggccgcgatc atcgacgcca gcacgatgcg
     1501 ctcgatgccg aagacgagaa cgacgagggt ggccgccacc aggaaataga actgcaggcc
     1561 cgagcccgtt ccgatggtgt aacagatgta tccgacggac aggtatgcga agacgaagaa
     1621 cgtcagtggt gcgatcagtt caccgaaccg gtacagcatc ggcaccgtca ggaaagcagc
     1681 cccgaacacc aggttgagca gccccaccca ccacgccggg ccgccgagca gcagttggaa
     1741 gatggcgaac gcgacgctga tggtcgccgc gatccacgtc gcgatcttga gcacgcggaa
     1801 ccggctggcg acgctgtcgg cgtagtgctg ggtccgggcc ggcaccgaag tcttcagtgc
     1861 cagctcaggc atgcaaaccg agcgcgccct gcgcgtcatg gcagaagcct acttcgcggg
     1921 gtcggccgat agcccggcta tgtcgcgagc gcgcagggcc gaccacagct gatgacatcg
     1981 ttggcagttt gctgaccagt ccactgcaag cgacgtgatg gcggagtggg accgcagcaa
     2041 gcagggcgca ggcgcaggct gagcaataac ggatgcgaca attcggtgcg gcgttgtaag
     2101 gttgcccgga accgttggct gatcgtgggg gcgagctttg gaccggtggt ggatccgtag
     2161 cgcggcagtc ggttgtgcgt ttgcctcgtg cgcaatggtc ggcgggccgg gcgtcgtcgg
     2221 ccccgcggtc gcacatgccg acctcttcgg tttcggtttc gatttctgcg gcggcgacga
     2281 caaatccgat ctgcaccatc cgcgcccggg ctccgaggtg agcgcacaaa caacgcggat
     2341 atccgccgtc gcggatgcag cagcgccgac ggcgaaggtc ggcagcctgc ccgagaacgt
     2401 cgacgcccct gcaagcgccg aactccgctc ggccgcgggc gttcccgaga acgtcggtgt
     2461 cgccgagagc gccggcggcg gcgcaggcgg agtgccccgc accaacatca cgggtcgggc
     2521 cgcaaatctt ccccgcgtgt catctgcccc ggtgacccgc agcatcgtga tccgcggagt
     2581 tccgcgagcc gtcgcccccg ccttcgtgcc gccgacactg cggcaagcgc ctgtcaccgc
     2641 cggtttggcc gcgccggcgc cggtctcacc ggaaccggag ggccagcctg cgcccgccgc
     2701 gccgccggct ccgtctccaa cggcgccgca aacgaaagat ccactcgcgt cgcgccattc
     2761 aggcatagcc cgaatctccg actcgtaccg ggccgggtac gcggaccacc tcagggcagc
     2821 gaccgtcagt gatctgttcg cggaagcact gccgggggtg gcgggcattg cggggttcac
     2881 cctcatcggc gcattcgcgg gctaccgcca ggcccgggcc ctgaaacgag cattgctcgc
     2941 ccctgtgccg acgagcgtct tgctgtagga cgtggtttag acctagccgg gttttgggta
     3001 tgtctctgcg gcgtttcgag gtgtctctca cggtaggagc acggagcatg atcaggccgg
     3061 cggcgcgagg tgcgattaga tgcgcgatgg cctgtggtgc cctgctcgtc ggcccatcgc
     3121 tggtcggtac cggtgtcgcg aaagcggatc tgctcggtgt cggcgggggt ggcggcggca
     3181 tcgacgtgct cggcgtcgac gtgttgggtg gtagggcgaa gggtagcggc gcctccggct
     3241 ccgcggcccg cctaagcgct gtctctactg cgcccagtac gcgcagtgtc gtgatccgcg
     3301 cgaaagaacc agcggcacaa cccgacccga cagccgtgcc ggcggctttc accgctcctg
     3361 cagccgaagc gcctgctgta gcgctgagcg cacccgttgt cgacgcgatt ccggccgcgc
     3421 caccgcccgc ggcgccgccg atggccgcga ccggtccgtt gtccatccag ccgcctgctg
     3481 cgccggccgt cctttcaccg gcgccgctgg ccgcaccggt catcccgtcg acgagtgcac
     3541 ctggtccggg tcgtccgctc ggacctgccg atatccatcc acgagtgacg aaggtgcccg
     3601 acagcttccg ggtcggctat ccggagtacc tgcgatcagc caccaacacc gatctgctcg
     3661 tcgcagccgt gcccggcgtc gcgggtatcg ccggattcac gctcatcggt gcattcgcgg
     3721 gctaccgcca ggccaaggca gtacagatgg cgtt
//
LOCUS       DASYLX010000463         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2653436,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000463 DASYLX010000000
VERSION     DASYLX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653436"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VGP27_11290"
     CDS             complement(<1..2138)
                     /locus_tag="VGP27_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7851925.1"
                     /translation="MSETSWSDPDIGLDMSELPTGTVTLLLADVEGSTRLWETQPVAM
                     KSAVERLDQTLSERVAAHRGVRPVEQGEGDSFVIAFARAGDAVACALDLQRAPLAPIK
                     LRIGVHTGDIHLRDEGNYIGPTINRTARLRDLAHGGQTVLSGATEPLVVDQLPPDASL
                     TDLGKHPLRDLPRPERVVQLCHPDLPSDFPPLRTANALATKHLPVQLSSFIGRQAEMS
                     AIRDALADNRLVTLTGAGGAGKTRLAIQVAVNIAPEFADGIWYVDLAPITDPEVVPVT
                     VARALSLPDQPRQQTMDVLLRFIRDRRMLMLLDNCEHLLDASADLITALLGACPALTL
                     LTTSREPIGVTGEVSWRVPSLSLADEALELFNDRARRTKPEFRITADNAETVTEICRR
                     LDGLALAIELAAARVRALSPAEILDSLHDQFRLLTGGARTAVRRQQTLRASVDWSYAL
                     LSEPEQVLFRRLAVFHGGFDVDAAQAVAGGAEAERYHVLDQLTLLLDKSLVVAEDTRH
                     GTRYRLLETVRQYAQEKLHESREGDDVRARHRDHYTAMAVLLDEPADSGYQERVERSE
                     TEMDNLRAAFAWSRESSDTELALTLASSLQPLWLTRGRIQEGLNWLSTALAGDSSQSA
                     GASAARVRASADKALLLSFTGSTEGREEAEQALATARELGDPALVVRALTSCGALANP
                     HDREVAGPFFAEATELAREHGDSRSLAQIIA"
BASE COUNT          394 a          742 c          742 g          382 t
ORIGIN      
        1 gcgataatct gcgccagcga ccgggaatcg ccatgctccc gcgcgagttc cgtcgcctcc
       61 gcgaagaacg gtcccgccac ctcacggtcg tgcgggttgg cgagcgcacc gcaggacgtc
      121 agggcccgca ccacaagcgc agggtcgccg agttcccgtg cagttgccag ggcctgctcg
      181 gcctcctcgc gcccctcggt agagccagta aacgacagca gcagagcttt gtccgcggaa
      241 gcccgcacgc gcgcagccga tgcgccggcg ctctgtgagc tatcgccggc aagcgcggtg
      301 ctaagccagt tcagcccttc ctggatgcgt ccccgcgtca accacagtgg ttgcagcgac
      361 gacgccaacg tcagcgccag ctcggtatcc gagctttccc gactccacgc gaaagcggct
      421 cgtagattgt ccatttcagt ttccgaccgc tcgacgcgct cttggtagcc gctgtccgca
      481 ggctcgtcca acaggacggc catcgcggtg taatggtcgc ggtgacgagc ccgcacatcg
      541 tcgccctcgc gcgactcgtg gagcttctcc tgcgcgtact gacgaacggt ttccaacagc
      601 cggtagcggg tgccatgtcg ggtgtcctct gcaaccacga gtgacttgtc gagcaacagc
      661 gtgagctggt cgagcacgtg gtagcgctcc gcctcggcgc cgccggcgac agcctgagcg
      721 gcgtcaacat cgaacccgcc gtggaaaacc gccagccttc ggaacaagac ctgttcgggc
      781 tcgctcagca gcgcgtacga ccagtccacc gatgcacgca atgtctgctg acgccgcaca
      841 gcggtacgcg cgccgccggt cagcagacgg aactggtcgt gcaggctgtc gaggatctcg
      901 gccggcgaca atgcccggac ccgagccgcg gctagctcga tggccagcgc aaggccgtcg
      961 agcctgcgac agatctcggt cacggtctcg gcattgtccg cagtgatacg gaattcgggt
     1021 ttggtcctgc gcgctcggtc attgaacaac tcgagcgctt cgtcggccag cgacagagaa
     1081 ggcacccgcc aactcacctc gcccgtcacg ccaatcggtt cgcgacttgt ggtcagcagc
     1141 gtcaacgcag ggcacgcgcc caacagggcg gtgatcaaat cggcgctggc gtcgagcagg
     1201 tgttcgcaat tgtcgagcag catcagcatc cgacggtcgc gaatgaaccg cagtagtaca
     1261 tccatcgtct gttggcgggg ttggtcgggc aggctcagcg cgcgtgccac cgtcactggc
     1321 accacctcgg gatccgtgat cggtgccagg tcgacgtacc agatgccatc ggcgaactca
     1381 ggggcaatgt tgacggcgac ctggatcgcc agccgcgtct tgcccgcgcc gcccgcgcct
     1441 gtcagtgtga cgagccggtt gtcggcgagc gcatcgcgga ttgcactcat ttcggcctgg
     1501 cgcccgatga aactcgacag ctgcactgga agatgcttgg tggcaagggc gttggctgtt
     1561 cgcaacggcg ggaaatcgct aggcagatcc gggtgacaaa gttgtacgac ccgttcgggg
     1621 cggggcagat cgcgcagcgg gtgcttgcca aggtcggtca gcgaggcgtc tggtgggagt
     1681 tggtcgacga caagcggttc cgtcgcaccg gagagcaccg tctggccgcc gtgcgcgagg
     1741 tcgcgcagcc gcgcggtgcg gttgatggtc gggccgatgt agttgccctc gtcgcgcagg
     1801 tggatgtcgc cggtgtgcac gccgatgcgc aacttgatcg gagccaacgg agctcgctgc
     1861 aaatccagcg cgcaggccac tgcgtcgccg gcacgcgcga acgcgatgac gaagctgtca
     1921 ccctcaccct gctcgacggg ccgcacacca cggtgggcgg cgacccgttc ggacagggtc
     1981 tggtcgaggc gttcgacggc ggacttcatc gccacgggct gggtttccca caggcgcgtc
     2041 gaaccttcga cgtcggccag cagcaacgtg accgttccag tcggaagctc actcatgtca
     2101 aggccaatgt ctgggtcgct ccagctagtt tcgctcatgc tagccagcat gcggtgacgc
     2161 gcagcgcaga acatcagcga tatcgccaat agttgcgacg caggcatgcg tacatacgca
     2221 tccggccatg acggatttag gcgcaatggc cgatggtcgc
//
LOCUS       DASYLX010000464         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1776657,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000464 DASYLX010000000
VERSION     DASYLX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776657"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VGP27_11295"
     CDS             <1..1479
                     /locus_tag="VGP27_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7851926.1"
                     /translation="APTDKVLHLSFLQDPGQPPDPDIYYAGQGLLLTTNIYEGLLQYK
                     ADTDRAELEPLLATEWKASPDNKVFTLKLRQGVKFHDGTPFTSAAVKASFDRRAAVNQ
                     GPAYMVTDVESVTTQGDYDVTITLKAPNSAFLDYLACPYGPRMLSPEGLKKNAGSDNA
                     QTYLTNHDLGTGPYTLTAAEVGSKYELAAFADYWGAKPYFEKVEMPVITDVSAQQLQF
                     NNGQIAAIMHDLPSSAVGQYLNETKYAHYSLPTMMSNYLYINPRKGMMTDAKNRNAVM
                     QAVDVDELVKQTYFGRGEKADQIYPSNMMPPEFGKQSVPHDPSVLTAVAAGLPPDQKS
                     VTIGYDSSNPDNQLISNLLQTQLAAAGLNAKVQAYPTSEIYGWIGSDAQSAPEIMAYL
                     GWPDAPSPYTWGHISWDGDGGLNFFGCSAPPVAAALAKGLPTGSQADYSTAGLEAVKT
                     GCWLNIANVNDFMVAQPWLKGVEQSHVVTNPNTLRLAALSVG"
     gene            1479..2303
                     /locus_tag="VGP27_11300"
     CDS             1479..2303
                     /locus_tag="VGP27_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV7851927.1"
                     /translation="MAVLVRKRGVRRIILLTLGWEELPKSVSVYGSSPELRMREPVPG
                     VLLQTDGGWVLLDTGFNTALIRDPALRRRYFPSVEYQPVLPGPGEPIEESLADIGVDI
                     DDIHLVGLSHLHVDHAGGLKLFAGKVPVHAQRREVEYGLSNHPEPESNAIFRVNFDDP
                     NIDWHLADGEAEIAPGITAVPTYGHTPGHQSFVVELDESVGGGGYVFAFDAADLTENI
                     EGELAIGGFIGVEPEETIEPIRRLKKLAGDKGYPLIPGHDPHVWPEMTRHFHERFG"
     gene            2300..3604
                     /gene="allB"
                     /locus_tag="VGP27_11305"
     CDS             2300..3604
                     /gene="allB"
                     /locus_tag="VGP27_11305"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEV7851928.1"
                     /translation="MTDLVVRAERVLLDGGLRSAAVAVADGRIAEIGAVDAKFPASVA
                     EVLMPTNAVLLPGFVDTHVHINEPGTDWEGFATATAAAAAGGITTLVDMPLDSDPVTT
                     SVEALRVKQAVAQSNCHVDVGFWAGVVPENLENLGPLAQAGVRGFKCFLSDSGNPNFS
                     HLTPDQLRKAMARIAELGSVLLVHAESHHIVGASSRPYGRHYKSFLESRPDAAEEDAV
                     ALVLGAAAASGARAHIVHVSSSLVLSRLAEAKRSGVPVTAETCPHYLTFAAESIPDGA
                     TEFAACPPIRNGANREPLWDGLLDGTLDMVVSDHSPCAPQHKADGDFGRAFGGVSSLQ
                     LGPRVIWTQATRRGFGLAELSRWMSERPAALAGFSDRGRIATGLRADLCAFDPETEEV
                     VDMSALRHRHQVSPYHRVALRGSVLQTWVAGHSVFGAVCEPA"
     gene            3601..>3968
                     /locus_tag="VGP27_11310"
     CDS             3601..>3968
                     /locus_tag="VGP27_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV7851929.1"
                     /translation="MKILVLNPNTSETMTAQIAAAARAVANAGTEIDCMAPCFGAAAV
                     DSAAESYLSAVGVMDTVALLLEEGRFHYDAVVLAGFGEHGKDALQEMLTVPVFDIAEC
                     AAHVAHLIGRRFSVVTTLSRS"
BASE COUNT          720 a         1278 c         1290 g          680 t
ORIGIN      
        1 gcacccacgg acaaggtcct gcacctgtcg ttcctgcagg accccggcca accgcccgat
       61 cccgacatct actacgcagg acagggtctc ctcctgacca ccaacatcta cgagggtttg
      121 ctgcaataca aggcggatac cgacagggct gaactggaac cgctcctggc caccgagtgg
      181 aaggcctcgc ccgacaacaa ggtgttcacc ctgaagttgc gccagggcgt gaagttccac
      241 gacggcacgc cgttcacatc ggcagcggtc aaggcctcgt tcgaccgacg ggccgcggtg
      301 aaccagggcc ccgcctacat ggtcacggac gtcgaatcgg tgaccacaca aggagattac
      361 gacgtcacca tcacgctcaa ggctccgaac tcggcgttcc tcgactatct ggcgtgcccg
      421 tacgggcctc ggatgttgag ccccgagggt ctgaagaaga acgcgggcag cgacaacgcg
      481 cagacctatc tgaccaatca cgacctaggg accggcccgt acacgctgac ggccgcggag
      541 gtcggctcga agtacgagct cgcggcgttt gcggactact ggggcgccaa gccctatttc
      601 gagaaggtcg agatgccggt gatcaccgat gtttcggcgc agcagctgca gttcaacaac
      661 ggtcagatcg ccgcgatcat gcacgacctg ccgtcctccg cggtgggcca gtacctcaac
      721 gagaccaagt acgcgcacta ctcgttgccc acgatgatgt cgaactacct ctacatcaac
      781 ccgcgtaagg ggatgatgac cgacgccaag aatcgcaacg ccgtcatgca ggcggtcgac
      841 gtggacgaac tagtcaagca gacctacttt ggacgtggcg agaaggccga ccagatctat
      901 ccgtcgaaca tgatgccgcc ggagttcggg aagcaatccg tcccgcacga cccctcggtg
      961 ttgaccgccg tggcagcggg attgccgccc gaccagaagt ccgtcaccat cggatacgac
     1021 tcgagcaacc cggacaacca gttgatcagc aacctgctgc aaacgcagct ggcggccgcg
     1081 ggactcaacg ctaaggtgca ggcctatccg acatcggaga tctatggctg gatcggttcc
     1141 gacgcgcagt ccgcaccgga gatcatggcg tatctggggt ggccagacgc accgtcgccg
     1201 tacacctggg gtcacatctc gtgggatggc gacggtggac tgaacttctt cgggtgctcg
     1261 gcgccgccgg tcgccgcggc gttggccaag ggcctcccga ccggttcaca agccgactac
     1321 tcgacggcag gcctcgaagc ggtcaagaca ggctgctggc tcaacatcgc caacgtcaac
     1381 gacttcatgg tcgctcagcc gtggctcaag ggtgtcgaac agtcccatgt cgtgacgaac
     1441 ccgaacacgc tgcgactggc cgcgctctcg gtgggctgat ggcggtactg gtgagaaagc
     1501 gcggcgtccg caggatcatc ctgctgacac tcggttggga ggagctgccg aaatccgttt
     1561 cggtgtacgg cagttcaccc gagctgcgga tgagggagcc cgtgcccggt gtacttctgc
     1621 agacagacgg gggatgggta ctactcgaca ccggtttcaa caccgcgctg atccgcgatc
     1681 ccgcactgcg ccgacgatac ttccccagcg ttgagtacca gccggtgctg cccggcccgg
     1741 gagaaccgat cgaggagtcg ttggccgaca tcggcgtcga catcgacgac atccacctcg
     1801 tcgggttgtc gcacctgcac gtcgaccacg ccgggggcct gaagttgttc gcgggcaagg
     1861 tgccggtgca cgctcagcgt cgcgaggtcg agtacggttt gtcgaatcac cctgagccgg
     1921 agagcaatgc gatcttccgg gtcaacttcg acgatccgaa tatcgattgg catttggctg
     1981 acggggaggc cgagatcgcg ccggggatca ccgccgtgcc cacgtacgga cacacgcccg
     2041 gccaccaaag cttcgtcgtc gaactcgacg agtccgtcgg cggcgggggc tacgtattcg
     2101 cgttcgatgc ggccgacctc acggagaaca tcgaaggcga attggcgatc ggcggcttca
     2161 tcggtgtcga gcccgaggag accattgagc ccatccgcag gctcaagaag ctggccggcg
     2221 acaagggtta tccactgata ccgggccacg atccacatgt gtggccggag atgacccggc
     2281 acttccacga gcggttcgga tgaccgatct tgtggtgcgc gccgagcggg ttctcctcga
     2341 cggcgggcta cggtcggcgg cggtcgcagt ggccgacggc cgcatcgcgg agatcggcgc
     2401 cgtcgacgcg aaattcccgg cgtccgtcgc cgaggtcctg atgccgacga atgcggtcct
     2461 gctgcccggc tttgtcgaca cccacgtgca catcaatgag ccgggaaccg attgggaggg
     2521 tttcgcgacc gccaccgcgg cagctgcggc cggagggatc accacactcg tggacatgcc
     2581 tcttgactcc gacccggtga ccacgtccgt cgaagcgttg cgggtgaaac aggcggttgc
     2641 acagtccaac tgtcatgtcg atgttggctt ctgggcgggc gtagtgccgg agaacctcga
     2701 gaatctcggg ccgctggctc aggcgggcgt gcgcgggttc aagtgctttc tcagcgattc
     2761 tggcaacccg aacttctctc acctgacgcc agatcagttg cgaaaggcaa tggcgcggat
     2821 cgccgaactc ggctcggtgc ttctggtgca cgccgagagc caccacatcg tcggcgcgag
     2881 ctcccggccg tacggaagac actacaagtc gtttctggaa tcgcgtcccg atgccgccga
     2941 ggaagacgcc gtagcgctgg tgctcggcgc cgcggccgcc agcggcgccc gcgcgcacat
     3001 cgtgcatgtc tccagttcgc ttgtgctttc gcgactggcc gaagccaagc ggtcgggggt
     3061 accggtcacc gccgaaacat gtccgcacta tctgactttc gccgcggaga gcattcccga
     3121 cggtgctacc gagttcgcgg cctgcccgcc cattcggaac ggcgccaacc gtgagccgct
     3181 gtgggacggc ctcctggacg gcacgctgga catggtggta tccgaccatt cgccttgcgc
     3241 tccgcagcac aaggccgacg gtgacttcgg gcgcgccttc ggcggggtga gctcactgca
     3301 gctcggtccc cgggtgatat ggacgcaggc cacgcgccgg ggtttcggcc tggccgagtt
     3361 gagccggtgg atgtccgagc ggcccgcggc gctcgccggg ttctccgacc gggggcggat
     3421 tgccaccggg ctgcgggccg acctgtgtgc cttcgacccc gaaacagaag aggtcgtcga
     3481 catgagtgcg ctgcggcatc ggcatcaggt gagcccgtat caccgcgtgg ctctgcgcgg
     3541 ttcggtgctg cagacctggg tggctggaca ctcggtcttc ggtgcggtgt gtgagccggc
     3601 atgaagattc ttgtactcaa ccccaacact tcggagacga tgactgctca gatcgccgcg
     3661 gcagctcggg ccgtcgcgaa tgccggcacg gaaatcgact gcatggcacc atgcttcggc
     3721 gcggcagcgg tcgactccgc cgccgaaagc tatctgtcgg ccgttggggt gatggacacc
     3781 gtcgcgctcc tgctcgaaga gggccgcttc cactacgacg cggttgtgct tgccggattc
     3841 ggcgagcacg gcaaggacgc gctgcaggag atgctgacgg taccggtttt cgacatcgcg
     3901 gagtgcgccg cgcacgtcgc ccacctcatc gggcgacggt tctcggtggt cacgacgctg
     3961 agccgttc
//
LOCUS       DASYLX010000465         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1337154,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000465 DASYLX010000000
VERSION     DASYLX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337154"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="VGP27_11315"
     CDS             complement(<1..>1611)
                     /locus_tag="VGP27_11315"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEV7851930.1"
                     /translation="VIAAMQAGNVVLMVQPPRGFGENPVAIYHDPDLPPSHHYLAAYH
                     WLNEGFGADAVVHLGKHGNLEWLPGKTLGMSAACGADAALGNLPLIYPFLVNDPGEGT
                     QAKRRAHATLVDHLIPPMARAESYGDIARLEQLLDEHANIAALDPGKLPAIRQQIWTL
                     MRAAEMDHDLGLAERPEDDSFDDMLLHVDGWLCEIKDVQIRDGLHVLGQAPTDAPQLD
                     LVLAILRARQLFGGDKTVPGLRQALGLAEDGNDDRAAVDEAENCARTLLSDMQRAGWE
                     PAVADRLTDNGDVAAILRFAATEVVPRLAGTAGEIDEILRALDGRFIAAGPSGSPLRG
                     LVNVLPTGRNFYSVDPKAVPSRLAWETGTAMADSLLERYRKDHGDWPRSVGLSVWGTS
                     AMRTSGDDIAEVLALLGVRPVWDDASRRVIDLEPITLAELGRPRIDVTVRISGFFRDA
                     FPHVVTMLDDAVAIVAGLDEPAEQNYVRAHADADIAEHGDRRRATTRVFGSKPGSYGA
                     GLLQLIDSRNWRDDADLAQVYTAWGGFAY"
BASE COUNT          236 a          553 c          561 g          261 t
ORIGIN      
        1 gtaggcgaaa ccgccccatg ccgtgtacac ctgcgcgaga tccgcatcgt cccgccagtt
       61 gcggctgtcg atcagttgca ggaggccggc gccgtagctg cccggcttcg agccgaaaac
      121 ccttgtggtg gcgcgccgtc ggtcgccatg ctcggcgatg tcggcgtccg cgtgcgcgcg
      181 gacgtagttc tgctcggcgg gctcgtcgag gccggcaacg atcgcgaccg cgtcgtcgag
      241 catggtgacc acgtgcggga aggcgtcccg gaagaagccg gagatccgca cggtgacgtc
      301 gatgcggggc ctgccgagtt cggcgagtgt gatcggctcg aggtcgatga cccgccgcga
      361 cgcgtcgtcc cataccggcc ggacccccag cagcgcaagc acttctgcga tgtcgtcgcc
      421 cgacgtgcgc atcgccgacg tgccccagac cgagaggccg accgaccgcg gccagtcgcc
      481 gtgatccttg cggtaccgct ccagcagcga gtcggccatc gcggtgccgg tctcccacgc
      541 cagccgtgac ggcactgcct tcgggtcgac ggagtagaag ttgcgtcccg tgggcagcac
      601 attgaccagg ccccgcagcg gtgaacccga cggacccgcg gcgatgaacc ggccgtcgag
      661 agcgcgcagg atctcgtcga tctcccccgc cgtgcccgcg agccgcggaa cgacttccgt
      721 cgccgcgaac cgcagtatcg ctgcgacgtc accattgtcg gtcagtcggt cggcgaccgc
      781 aggctcccag ccggcccgtt gcatgtcgga aagcagtgtg cgcgcacagt tttcggcctc
      841 gtcgacggcg gcgcggtcgt cgttgccgtc ctcggccaga ccgagcgcct gtctcaggcc
      901 gggcaccgtc ttgtcaccgc cgaacagctg gcgggcccgc agtatggcga ggacgaggtc
      961 gagctgcggt gcgtcggtcg gcgcctgacc cagcacgtgc agaccgtcgc ggatctggac
     1021 gtccttgatc tcgcacagcc agccgtcgac atgcaacagc atgtcgtcga acgagtcgtc
     1081 ctccggccgc tcggccaaac ccaggtcatg gtccatctcg gcggcgcgca tcagcgtcca
     1141 gatctgctgg cggatcgccg gcagcttgcc cgggtcgagt gcggcgatgt tggcatgctc
     1201 gtcgagcaac tgctccaaac gcgcgatatc cccgtagctt tcggcgcggg ccatcggcgg
     1261 gatgaggtgg tcgacgagtg tggcgtgtgc gcgccgcttg gcctgcgtgc cctcgcccgg
     1321 gtcgttgacg aggaacggat agatcagcgg caggttgccc agtgctgcgt cggctccgca
     1381 cgccgcggac atgccgagcg tcttgccggg cagccactcg aggttgccgt gcttgccgag
     1441 gtggacgacc gcgtcggcgc cgaaaccttc attcagccag tggtaggccg ccaggtaatg
     1501 atggctcggc ggcaggtccg ggtcgtggta gatggctacc gggttctcgc cgaagccccg
     1561 cggtggctgc accatcagca cgacgttgcc cgcctgcatc gcggcgatga c
//
LOCUS       DASYLX010000466         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1447249,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000466 DASYLX010000000
VERSION     DASYLX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447249"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VGP27_11320"
     CDS             <1..101
                     /locus_tag="VGP27_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7851931.1"
                     /translation="AYVTKPVDLDQFMSAVRQIDEFFVQVVRLPKP"
     gene            complement(112..564)
                     /locus_tag="VGP27_11325"
     CDS             complement(112..564)
                     /locus_tag="VGP27_11325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7851932.1"
                     /translation="MADFGQRDSRTRFAHTSVLADAACAAHLRQEFSVWLRRWFSLDP
                     RRVSDMVLAVNEALANAAEFAYSAVEHSGAMHLRADYDTGSAVLTVTVTDEGAWRLKD
                     AANEHVSRGRGIPLMRALADRATIDSTPAGTRVCLEWNHVAAAQSAVT"
     gene            complement(597..956)
                     /locus_tag="VGP27_11330"
     CDS             complement(597..956)
                     /locus_tag="VGP27_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7851933.1"
                     /translation="MEGAASSSCAIEERRIEAVSVVSVSGVVDMLTAPDLETAISEAA
                     KSSPAAVIVDMTAVEFLASAGMGVLIAAQDELAPAIKLAVVADGPATSRPLKLVGVTD
                     VVDLYATLDEALAALTN"
     gene            complement(1056..>1731)
                     /locus_tag="VGP27_11335"
     CDS             complement(1056..>1731)
                     /locus_tag="VGP27_11335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7851934.1"
                     /translation="GPLVREIVSAATGKSIRDILATEILDPLGFRWTNFGVAEHDVPL
                     VAPSHTTGKPLPAPIAKAFKMAVGGTMAEIIPFSNTPLFLTGIVPSSNLVSTADEMSR
                     FAEILRRGGELDGVRVMRPETLRAATKECRRLRPDIADGFAPRRWGTGYMLGNKRFGP
                     YGRNAPAAFGNTGLTNIAVWADPERGLAAGLISSGKPSGHREAKRYPALLDRITAEIP
                     RRRIQS"
BASE COUNT          272 a          584 c          577 g          298 t
ORIGIN      
        1 acgcctacgt caccaagccg gtcgatctgg accagttcat gagtgccgtg cgccagatcg
       61 acgagttctt tgttcaggtg gtgcggctgc cgaagccgtg aggccgggcc gtcaggtgac
      121 agcggactgc gccgcggcga catggttcca ttccaggcac acccgcgtgc ccgcgggcgt
      181 cgagtcgatg gtggcccggt cggcgagtgc gcgcatcagc gggatgccgc ggccgcgcga
      241 gacgtgttcg ttcgcggcgt ctttgagccg ccaggcgccc tcgtcggtca cggtcaccgt
      301 caagactgcc gaaccggtgt cgtaatcggc ccgcagatgc atcgcgccgg aatgctcgac
      361 ggccgaatag gcgaactcgg cggcattggc caacgcttcg ttgaccgcca gcaccatgtc
      421 gctgacccgt ctcgggtcga gcgagaacca tcgccgcagc cacaccgaga actcctgccg
      481 caagtgcgcg gcgcatgcgg catcagcgag gacactcgtg tgtgcgaacc gtgttctcga
      541 gtcgcgttgc ccgaaatctg ccatctcagc ctgctggcta cccgatactg acccttttaa
      601 ttcgtcagtg cagcgagcgc ttcgtcgagg gtggcgtaca gatcgacgac atcggtgacg
      661 ccgacgagct tcagcggtct gctggtcgcg gggccgtccg ccacgaccgc caacttgatg
      721 gcgggggcga gctcgtcctg ggcggcgatc aggacgccca tgcccgcgga tgcgaggaac
      781 tccacagcgg tcatgtcgac gatcacagcg gccggggaac tcttggcggc ctcggatatc
      841 gccgtttcga ggtcgggggc tgtgagcatg tcgaccacac cggataccga caccacgctg
      901 acggcctcga tgcggcgttc ctctattgcg cagctacttg atgccgcacc ttccatgctc
      961 acctccacaa atgcccgtcc gccccatcgg cggcggcatg gagagtttac cgggagtacc
     1021 ggaatacgtc gcagatgggc gggttgcccc ctgcctcagg attggatccg ccgacgcggg
     1081 atctcggccg tgatgcggtc gagcagggcc gggtagcgct tggcttcgcg gtgaccgctc
     1141 ggcttgccgc tgctgatgag cccggcggcc agaccccgct cggggtctgc ccagaccgcg
     1201 atgttcgtca ggccggtgtt gccgaatgcc gcgggcgcat tgcggccgta cggtccgaac
     1261 cgcttgttcc ccagcatgta gccggtgccc cagcggcggg gcgcgaatcc gtcggcgatg
     1321 tcgggccgca gccgcctgca ctccttggtc gccgcccgca gggtctccgg ccgcatcacc
     1381 cggacaccgt ccaactcacc accccgtcgc aggatctcgg cgaaccgtga catctcgtcg
     1441 gccgtcgaga cgagattgga tgacggcacg atcccggtca ggaacagcgg cgtgttcgag
     1501 aacgggatga tctccgccat ggtcccgccg accgccatct tgaacgcctt cgcgatcggc
     1561 gcgggaagcg gcttgccggt ggtgtggctg ggcgccacca gcggcacgtc gtgctcggcc
     1621 acaccgaagt tcgtccaccg gaagccgagc ggatcgagga tctcggtggc aaggatgtcg
     1681 cgaatactct ttcccgttgc cgccgagacg atttcccgca ccagcgggcc c
//
LOCUS       DASYLX010000467         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3202017,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000467 DASYLX010000000
VERSION     DASYLX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202017"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1117)
                     /locus_tag="VGP27_11340"
     CDS             complement(116..1117)
                     /locus_tag="VGP27_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851935.1"
                     /translation="MGLTWWPVAVVGFVCLAAAVALAMMMPMEQVRRRLRRLANSSRL
                     TRLPEYARMARARLVSMVVAVVLLGLLFGTAVVASARPSGWWWSSAATDPPQDIMLCV
                     GQPVTDKSTGAFLTYFANQARTYGTQRIGLTSPNRRVIPMTRDYQFVAGRLGDLARLS
                     ERPADNPAGATASFSPAVSYVDYAASVEDILALCMTGFPSFDSKSSHRRSLVYLGSGQ
                     TRDPEESRRSLLTAEQVAGMASNAGIQINALSASSREAGPLPSIAKSSGGQYFSLENR
                     ENQLTADLDAIRDRPPTAGAPADEAVTGWFGDAPAVPLAVAVVISLLLCLSVMVLRR"
     gene            complement(1108..1581)
                     /locus_tag="VGP27_11345"
     CDS             complement(1108..1581)
                     /locus_tag="VGP27_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851936.1"
                     /translation="MPDDILRFVIGPSSYAVWWLWAGLALLLVVIAWYVGVFVWTLPA
                     DRLRSIPWIRTLHSKLLRRRFSRAIRAIDVRYRTGELSAAEAGHQMSRTLRSFLALAT
                     STRAQYMHVADIRSSDLAEAAPLFAAFNDAQFNTASQVEISRVGSTAEELIRSWA"
     gene            complement(1571..>2270)
                     /locus_tag="VGP27_11350"
     CDS             complement(1571..>2270)
                     /locus_tag="VGP27_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7851937.1"
                     /translation="DEKHHKILLVADAGRNMTAQAPSGEAKIDVAANILGAIGLITLG
                     RADQIGMVYGDTRGCVNIRQGRGETHIESMLHRFYGHTLSTPGPSDIFCQLEYVATHY
                     RHSMLIVVVSDEPEIDARLEDVVRRLAGRHNVMWAMVSDAPAIGTPDATDDGYDVTTG
                     RFVIGGASLSPRIIAAYRRAEENRKRELQEFMTTRAIPHTTVGGSRQIRSRLVAMSQV
                     FASSSRAGGHARAG"
BASE COUNT          411 a          734 c          769 g          356 t
ORIGIN      
        1 gtcgtccatc gcgcccctgc agtggtgtgc agacgccgca tggtgagcag ccgcaacgcg
       61 atgaccacga cggcgatcac gagcagcagg gcagcaggca gcgcaggctg gaacgtcatc
      121 gtctcagcac catcaccgac aggcacagca gcagcgagat gaccacggcg accgcaaggg
      181 gaacggccgg cgcatcaccg aaccagccgg tgacggcctc atcggcaggg gcgccggcag
      241 tcggtggcct gtcccgaatg gcgtcaaggt cggcggtgag ctggttttcg cgattctcga
      301 gcgaaaagta ctgaccgccc gacgatttcg ctatcgacgg cagcggcccg gcctcgcggc
      361 tcgatgcgct gagcgcgttg atctggatgc cggcattgct cgccatgccg gcgacctgtt
      421 cggctgtcag cagcgatcgc cgggactctt ctggatcgcg agtctgtccg gaccccaggt
      481 agacgagcga acgacggtgt gagctcttgg agtcgaacga cgggaatccg gtcatacaca
      541 gcgccaggat gtcctcgacg ctggccgcgt agtcgacata ggacaccgcg ggggagaagg
      601 atgcggtggc gcctgcggga ttgtcggccg gcctctcgga gagccgggca aggtcgccga
      661 gcctgcccgc cacgaactgg tagtcccggg tcatcggaat gacgcgacga ttcggcgacg
      721 tcagcccgat gcgctgggtg ccatacgtcc gcgcctggtt ggcgaaatag gtgaggaagg
      781 caccggtgga tttatcggtg accggttgac cgacgcacag catgatgtcc tgcgggggat
      841 cggtcgccgc gctcgaccac caccatccgc tcgggcgggc gctggccacc accgcggtgc
      901 cgaacaacag cccgagcagg acaacggcga cgaccatcga gacgagccgt gcccgcgcca
      961 tccgggcgta ctcgggtagc cgcgtgagcc gcgacgagtt cgccagccgg cgcagccggc
     1021 gccgaacctg ctccatcggc atcatcatcg ccagcgcgac agccgctgcc agacagacga
     1081 atccaacgac agcgacaggc caccacgtca ggcccacgaa cggatcaact cctcggcggt
     1141 cgagcccacc cggctgatct cgacctgcga cgcggtgttg aactgggcat cgttgaaagc
     1201 cgcgaacagc ggagccgcct cggccagatc gctggacctg atgtccgcga cgtgcatgta
     1261 ctgcgctcga gtgcttgtcg ccagggccag gaagctgcgt agcgtccggc tcatctggtg
     1321 accggcctct gccgcggaca gttcaccggt tcgatagcgc acgtcgatag cgcggatggc
     1381 gcgggaaaat ctgcgccgca gcaacttcga gtgcagagtc cggatccacg ggatcgaccg
     1441 gaggcggtcg gcgggcaatg tccagacgaa gaccccgaca taccaggcga tgacgaccaa
     1501 cagcagcgcc agcccggccc acagccacca caccgcatac gacgaaggtc cgatcacgaa
     1561 gcgcaagatg tcatccggca cgcgcgtgcc ctcccgctcg tgaggacgac gcaaagactt
     1621 gactcatcgc gaccagccgg gaccggatct gtcggctgcc gccgacggtt gtgtggggga
     1681 tcgcgcgggt ggtcatgaac tcctgcagtt cacgcttccg gttctcttcg gcccgtcggt
     1741 aggccgcgat gatccgcggg ctcaggctgg caccgccgat gacgaatctg ccggtcgtga
     1801 cgtcgtatcc gtcgtcagtg gcgtcgggcg tgccgattgc gggggcgtcg gagaccatcg
     1861 cccacatgac gttgtgccga cccgcgagac gacggaccac gtcctcgaga cgggcgtcta
     1921 tctcgggttc gtcggatacc acgacgatga gcatcgagtg acggtaatgc gttgccacat
     1981 actccaattg gcagaagatg tcgctggggc cgggcgtcga cagagtgtga ccgtagaacc
     2041 ggtgcagcat gctctcgatg tgggtttcgc cgcggccctg ccggatgttg acgcagccgc
     2101 gggtgtcgcc gtacaccatg ccgatctggt cggcgcggcc aagagtgatc agcccgattg
     2161 cgccgagaat gttggcggcg acatcgatct tcgcctcacc gctgggtgcc tgggcggtca
     2221 tgttgcggcc cgcgtcagcc accagcagga tcttgtggtg cttctcgtcg
//
LOCUS       DASYLX010000468        10163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3531793,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000468 DASYLX010000000
VERSION     DASYLX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531793"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..301
                     /locus_tag="VGP27_11355"
     CDS             20..301
                     /locus_tag="VGP27_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEV7851938.1"
                     /translation="MCRNITELRGLEPAATAEEIEAAARQYIRKVSGVTRPTAANAEA
                     FETAVGEVTAVTTRLLAALPARRQPPKTVPPLRRPEVRARIAAKAAGAG"
     gene            313..861
                     /locus_tag="VGP27_11360"
     CDS             313..861
                     /locus_tag="VGP27_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEV7851939.1"
                     /translation="MTQPALKEWSAAVHAMLEGRQTVLLRKGGIHEKRFTVDSPHFLF
                     FPTVAHSHAQRVRPEYRQLLAPAAADSSEAELVLRAGAKVVAAVEVTRPEGLEDIASL
                     HIWTADSVRADRLDFRPKHRLTVLVVQVSPLAEPERLRRAPEYAGCTSWVQLPVNPMW
                     ATPVHDDATLRDVAARVRESVA"
     gene            complement(850..2487)
                     /locus_tag="VGP27_11365"
     CDS             complement(850..2487)
                     /locus_tag="VGP27_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV7851940.1"
                     /translation="MTNARNLVGRTLGRILGLPPHTTDYQVAHRVRVPMRDGVDLVAD
                     HYRPLTSNPAGTLLVRTPYGRGYPVSTLFGGVHASRGYHVVVQSVRGTFGSGGNFNPF
                     MHEVTDGADTAAWLRDQPWFTGSFGTIGASYLGFTQWALLTEPPPEMAAAVIIVGPHD
                     ISGPRWGTGSFGLNDFLGWSDSVSHQEDPGRIRNIVRTVRSRRLLAQATAALPVGEAA
                     RALLGEGAPWFESWIEHSEHDHPFWEPVQLQAALQRSEVPVLLLTGWQDLFIEQTLEQ
                     YTQLRRRDVPVAMTIGSWTHSHMLTTGAPTVLRESLAWLDTHLAGNRGAERSPVRIRV
                     NHDDWLGLEDWPPPMPEQVRYLQPGGRLGAAMPPDTAPASTFTYDPADPTPTIGGRLL
                     SPVGGYRNDGRLAERTDVLTFTGDALPHDVYVVGNPALELSHSCDNPHNDLFVRVSEV
                     DVKGRSSNVSDGYRRETTSLGTVTVELDPVAHRFRAGSRIRVLVAGGSHPRFARNLGT
                     DEPLATGRRLRPATHTVHLGDGASRLRLPAGALPSSH"
     gene            complement(2568..3173)
                     /locus_tag="VGP27_11370"
     CDS             complement(2568..3173)
                     /locus_tag="VGP27_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEV7851941.1"
                     /translation="MATKIRLLAAFGALLTAVILVMQSGPLTVPAPGPVELRSTAAPL
                     PMATTTIKWPVIDTVDPDPFDPCRDIPLDVVARIGLAFTPPEPENSLRCHYDAGNYQV
                     AVEAFVWRTYEASLPPDAVELDVDGHRAAQFWVMKPTDWNNRWWTTCAIAFKTDFGLI
                     QQSLFYAPTYTADDVDCMQTNLQRAHELVPYYKFKGDPTTQ"
     gene            3306..4250
                     /locus_tag="VGP27_11375"
     CDS             3306..4250
                     /locus_tag="VGP27_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7851942.1"
                     /translation="MATLWAISDLHTGHTGNKPVTESLHPSSPDDWLIVAGDVAERTD
                     EIRWALDLLRKRFAKVIWVPGNHELWTTTKDPMQVFGRARYDYLVNMCDEMGVITPEH
                     PFPVWTDQGGPATIVPMFLLYDYTFLPEGAATKAEGLSIARENNVVATDEFVLSNEPY
                     ATKDAWCRDRVEATRKRLEDLDWVTPTVLVNHFPLVREPCDALFYPEFSLWCGTTATA
                     DWHTRYNAICSVYGHLHIPRTTWYDGVRFEEVSVGYPREWRRRKPYRWMRQVLPDPEY
                     PSGYLNEFGGHFEITQEMRTNAQKVQERIKNRQATRSR"
     gene            4247..4942
                     /locus_tag="VGP27_11380"
     CDS             4247..4942
                     /locus_tag="VGP27_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEV7851943.1"
                     /translation="MIVAPLLSGVLPRTRGAQQGEVSVAAAELYSDPPDLAPLPEEEP
                     LIAKSVAKRRNEFVTVRYCARQALEELGVPPGPILKGEKGEPCWPDGVVGSLTHTEGF
                     RGAAVARHNDVRSIGIDAEPHDVLPNGVLDAISLPAERAEISALPQALHWDRILFCAK
                     EATYKAWFPVTRRWLGFEDAHIVFDVDEGATAGGFESRILIDPAARNGPPLSTLRGRW
                     SVRNGLALTAIVL"
     gene            5065..5928
                     /gene="truB"
                     /locus_tag="VGP27_11385"
     CDS             5065..5928
                     /gene="truB"
                     /locus_tag="VGP27_11385"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV7851944.1"
                     /translation="MVVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVVG
                     IERATKILGLLIATDKSYAATIRLGQTTTTEDADGEVLQTVSAANVTDTDIEAAVDAL
                     RGDIAQIPSAVSAIKVGGKRAYQLAREGQTVELAARPVRIDRFAVCDVRRAADLIDVD
                     VEVDCSSGTYIRALARDVGAALKVGGHLTALRRTRVGRFGLDEARPLDELAEKPALSY
                     SLDEACLLAFPRRDLTADEAESARHGRALHPAGIDGVYAARDSDGHVISLLRDEAAKA
                     KSVVVLRPATL"
     gene            5983..9033
                     /locus_tag="VGP27_11390"
     CDS             5983..9033
                     /locus_tag="VGP27_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7851945.1"
                     /translation="MSGLLPTGMATLLLADVEGSTRLWETQPEEMRAALAQLNHTVSE
                     VIAAYGGVRPVEQGEGDSFVAAFTRASDAVACAVQLQRAPLDPIRLRIGMHTGEVQLR
                     DEGNYAGSTINRTARLRDLAHGGQTVLSGATEDMVVDRLPAGAWLADLGTHQLRDLPR
                     PERVMQLCHPDLEREFPPLRTAKRVGVHNLPVQLTNFVGREAEIDDVRRLLVDNRLVV
                     LTGAGGAGKTRLAVEIAARIAPEFGDGVWYVDLAPITHPAVVPVTVARALGLPDQPGR
                     STMDTLLRFVRDRPMLVVLDNCEHLLDAAAELVVALLGEAPGLAVLATSREPIGVAGE
                     ATWRVPSLSVNDAAIALFADRARLAQTGFAINSENSAAVAEICGRLDGMPLAIELAAA
                     RVRALSLAEILDGLHDRFRLLTGGSRTAVRRQQTLRASVDWSHALLTEPERVLFRRLA
                     AFLAGFDLDAAQAVVGDGDVQRYQVLDQLTLLVDKSLVVTENSSGRTRYRMLETVRQY
                     ALEKLAESGEADVVRARHRDHYTAMAALLDAPARTDYERRVEQAEEEMDNLRAAFVWS
                     LETGDVGRSLELATSLQPLWFSRGRIREGLAWFDAAFNGGNERHVDVPDAVRAGALAD
                     KAVLEAWVAAKFSLDQAEQALAIAREVEDPALVTRTLTACGAVAGYHDREVAQSYFTE
                     AIGLARAIGDQWRLSQILALEAQGATIAGDPLAARAAAEEGRDLADAIGDGSNSRQCR
                     FWLGLAQGANGDLAGADKQLSEVTAESAAAHDEIWRLSSVGSRGVILAYQGHAAGARA
                     AAEATLEGGAEIGDVLVGMGYHVLTLAALAAGDGAGAQEAGEAAWKHISVAGEMGAMW
                     RYWGGEAALAGGDLAVARCWADEAVSATRGIYLSWALTGRARVLMAEGQSRQAESDAH
                     DALARAAENQARICVPDVLEILADLAGKSGRRPLAARLFGAAQGIRQRIGVVRFKIYD
                     NGYQTSVSSLRDAMAEKDFDAAWAEGAALSTEEAIAYAQSVRRG"
     gene            9129..9563
                     /locus_tag="VGP27_11395"
     CDS             9129..9563
                     /locus_tag="VGP27_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851946.1"
                     /translation="MPVDRQTEAVARALLLCVGLLRRRLRQTPVTGELTFPETAALGR
                     LDRGGPAAAADLARQEQISPQSMGATLAELEARGLVKRQPDPGDGRRILLSISAAGRK
                     ELNRRRNARVEQLTAGLADFTEAELEQLAATAPLIERLAYHL"
     gene            9564..>10163
                     /locus_tag="VGP27_11400"
     CDS             9564..>10163
                     /locus_tag="VGP27_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851947.1"
                     /translation="MSVDEQETPTRDNYRWVALTNTTAAVFMSALDGSIVIISLPAIF
                     RGIHLDPLAPANITYLLWMIMGYRLVQSVLVVTLGRIGDMYGRVRVYNAGFTVFTVAS
                     ILLSFDPFDGSAAAQWLIGWRLLQAVGGSMLTANSAAILTDAFPPDRRGFALGFNQVA
                     ALAGQFIGLVAGGLLAAWDWRAVFWVNVPVGVFGTIWAYR"
BASE COUNT         1658 a         3307 c         3598 g         1600 t
ORIGIN      
        1 ctgaacctat cctggacgaa tgtgccgaaa catcaccgag ctccgcggcc tggaaccggc
       61 cgcgaccgcc gaggagatcg aggccgccgc gcggcagtac atccgcaagg tgagcggcgt
      121 gacgcggccg acggcagcca acgccgaggc attcgagaca gcggtcggcg aggtcaccgc
      181 ggtcacgact cgactgctcg cggcgctacc ggcgcgcaga cagccaccca agaccgtccc
      241 gccgttgcgg cgcccggagg tacgcgcccg catagcggcc aaggccgccg gcgccgggtg
      301 acagcgcaaa cgctgaccca acccgcgctc aaggaatgga gcgcggcggt gcacgcgatg
      361 ctcgagggcc gccagacggt gctgttgcgc aagggcggaa tccacgagaa gcggttcacg
      421 gtcgactcgc cgcacttcct gttcttcccg acggtcgccc acagccacgc gcagcgggta
      481 cggccagagt atcggcaact gctggcacct gcggccgccg acagcagcga ggccgagctc
      541 gtgctgcggg caggcgctaa agtggtcgcc gccgtcgagg tcacccggcc cgagggactg
      601 gaagacattg catcgcttca catttggacc gccgattcgg tgcgggcgga tcggctggac
      661 ttccggccga aacaccggtt gaccgtgctg gtggttcagg tcagcccgct ggcagagccg
      721 gaacgcctgc ggcgcgcgcc ggaatatgcg ggctgcacga gttgggtgca gctgccggtc
      781 aacccgatgt gggccacgcc ggtgcacgac gacgcaactc tgcgcgacgt cgcggcgcgc
      841 gtgcgcgaat cagtggcttg acggcagggc gcccgcgggc agtcgcagcc gcgacgcgcc
      901 gtctccgagg tgaacggtgt gcgtggcagg ccgcagccgc ctcccggtgg caagtggctc
      961 gtcggtgccg aggttgcgcg cgaagcgcgg gtgcgagcca cccgcgacca gcacccggat
     1021 ccgtgagccg gcgcggaatc ggtgcgcgac ggggtcgagt tcgacggtca cagtgcccaa
     1081 agatgttgtc tcgcggcggt atccgtcgct gacattgctg gaccttccct tgacgtcgac
     1141 ctcgctgacg cgaacgaaca aatcgttgtg cgggttgtcg caggagtgcg agagctcgag
     1201 ggccgggttc ccgacgacgt agacgtcgtg cggcagcgcg tcgccggtga aggtcagcac
     1261 gtcggtgcgc tcggcgagtc ggccgtcgtt gcggtacccc ccgacggggg agagcagccg
     1321 acccccgatc gtcggcgtcg ggtcggcggg gtcgtaggtg aaggtggacg cgggtgcggt
     1381 gtcgggtggc atcgcagcgc cgaggcggcc gccgggctgg aggtagcgca cctgctcggg
     1441 catcgggggt ggccagtcct cgaggccgag ccagtcgtcg tgattgacgc gaatccgcac
     1501 ggggctgcgc tcggcgccgc ggttacccgc cagatgcgtg tcgagccacg ccagcgactc
     1561 gcgcagcacg gtcggcgcac ccgtggtgag catgtgcgag tgggtccatg atccgatggt
     1621 catcgccacc ggtacgtctc gcctgcgcaa ttgggtgtac tgctccagcg tctgctcgat
     1681 gaacaggtcc tgccacccgg tgagcaacag aacggggact tcggatcgct gcagcgcggc
     1741 ctgcagctgg accggctccc agaacgggtg gtcgtgctcc gaatgctcga tccaggactc
     1801 gaaccagggc gcgccctcgc cgagcagcgc gcgggccgcc tcgccgacgg ggagggccgc
     1861 cgtggcctgt gccagcagcc gccgggacct gaccgtgcgt acgatgttcc ggatccggcc
     1921 ggggtcctcc tggtgggaga ctgagtcgct ccacccgagg aaatcgttga gcccgaacga
     1981 accggtgccc caacgcggtc cgctgatgtc gtgcgggccg acgatgatca ccgcggccgc
     2041 catctccggc ggcggctcgg tcagcagtgc ccactgcgtg aagccgaggt aggacgcgcc
     2101 aatggtgccg aacgatccgg tgaaccacgg ctggtcgcgc agccacgcgg cggtgtcggc
     2161 gccgtcggtg acctcgtgca tgaacgggtt gaagttgccg ccggagccga acgtgccgcg
     2221 aacgctctgc acgacgacgt ggtagccgcg ggacgcgtgg acgccgccga acagcgtcga
     2281 aacgggatac ccgcggccgt agggcgtgcg gaccagaagg gtgcccgccg gattcgacgt
     2341 cagggggcgg tagtggtcgg cgaccaagtc cacgccatct ctcatcggca cccgcactcg
     2401 gtgagccacc tggtaatcgg tggtgtgcgg cggcaggccc agtatgcggc caagggtgcg
     2461 accgacgaga ttcctggcgt tggtcactgg aaccacgcta cgcaggcgcc gggtcgccat
     2521 cgagcgaacg ggagccgagc tcgagcgctc cagcccgacg ggcacgatca ctgggtcgtc
     2581 gggtcgccct tgaacttgta gtacggcacc agttcgtgcg cgcgctgcag gttggtctgc
     2641 atgcagtcga cgtcgtcggc ggtgtaggtc ggcgcgtaga acagcgactg ctgaatcagc
     2701 ccgaagtcgg tcttgaacgc gatcgcacag gtggtccacc accggttgtt ccagtcggtc
     2761 ggcttcatca cccagaactg ggccgcgcgg tgaccgtcga cgtcgagttc gacggcgtcg
     2821 ggcggcaggc tcgcctcgta ggtgcgccag acgaaggcct cgaccgccac ctgatagttc
     2881 ccggcgtcgt agtgacagcg caggctgttc tccggctccg gaggggtgaa cgcgagaccg
     2941 atgcgcgcca cgacgtctaa cggaatgtcg cggcacgggt cgaacggatc ggggtcgacg
     3001 gtatcgatca ccggccactt gatggtcgtg gttgccatcg gcagcggcgc tgcggtcgag
     3061 cgcagctcga ccggtcccgg cgcaggcacg gtcagcggtc cgctctgcat gaccagaatc
     3121 accgccgtca acagcgcgcc gaacgccgcg agcagacgga tcttggtggc cataacaccc
     3181 cctcgcgcat tgtcgagccc tgcggggact atacaagtcg ccgcaatggc gccggaccga
     3241 aaacgagaac atgttctagt tgcgcgggcg ggcgtcaccg ctgcgcttaa gtacgctggt
     3301 cggttgtggc gacactctgg gcgatcagcg acctgcacac gggtcacacc ggcaacaagc
     3361 cggtcaccga gtcgctgcac ccgtcatcgc cggacgactg gctgatcgtc gcgggcgacg
     3421 tcgccgagcg caccgacgag atccgctggg cgctcgacct gctgcgcaag cggttcgcca
     3481 aggtcatctg ggtgccgggc aaccacgaac tgtggaccac caccaaggac ccgatgcagg
     3541 tgttcggccg ggcgcgctac gactatctgg tcaacatgtg cgacgagatg ggcgtgatca
     3601 cccccgagca tccgtttccg gtgtggaccg atcagggcgg cccggccacg atcgtgccga
     3661 tgttcctgct gtacgactac acattcctgc ccgagggcgc ggcgacgaag gccgagggcc
     3721 tctccatcgc cagggagaac aacgtggtgg ccaccgacga gttcgtgctg tccaacgagc
     3781 cgtacgcgac aaaggacgca tggtgccgcg accgggtgga ggccacccgc aagcgcctgg
     3841 aggacctcga ctgggtgacg ccgacagtgc tggtgaacca cttcccgttg gtgcgtgagc
     3901 cgtgcgacgc gctcttctat ccggagttct cgctgtggtg cggcaccacg gcgaccgctg
     3961 actggcacac ccgctacaac gcgatctgtt cggtgtacgg gcacctgcac attccccgga
     4021 ccacgtggta cgacggcgtg cggttcgaag aggtgtcggt cggctacccg cgggaatggc
     4081 ggcgccgcaa gccgtatcgc tggatgcgtc aggtgctgcc ggatccggag tatccgtccg
     4141 ggtatctcaa cgagttcggc ggccacttcg agatcaccca ggagatgcgg acgaacgcgc
     4201 agaaggtcca ggaacgcatc aagaaccggc aggcgacgcg ctcgagatga tcgtcgcgcc
     4261 actgctttcc ggcgtgctgc cgcgcacgcg aggagcacaa cagggtgaag tcagcgtggc
     4321 cgccgccgag ttatacagcg atccaccgga tttggcgccg ctgcccgagg aggagccgct
     4381 gatcgcaaag tcggtggcca agcggcgcaa cgaattcgtc accgtccgtt actgtgcgcg
     4441 gcaggcgctg gaggaactcg gcgtgccgcc gggcccgatc ctcaaggggg agaagggcga
     4501 accgtgctgg ccggacggcg tcgtcggcag ccttacccac actgagggct ttcgcggcgc
     4561 ggcggtcgcg cgccacaacg atgtgcgatc gatcggcatc gacgccgaac ctcacgatgt
     4621 acttcccaac ggcgtgctcg acgcgatcag tctgcccgcc gagcgggccg agatcagcgc
     4681 tcttccgcag gccctgcact gggatcgaat tctgttctgc gccaaggagg cgacgtacaa
     4741 ggcgtggttt ccggtcaccc ggcggtggct gggtttcgag gacgcccaca tcgtcttcga
     4801 cgtcgacgaa ggtgcgaccg cgggtggctt cgaatcccga atcctgatcg accccgctgc
     4861 ccgaaacgga ccgccgctga gcacgctgcg gggccgctgg tctgtgcgca acgggctggc
     4921 gttgacggcg atcgtgctgt gaaagatccc gcgcacgcga ggagcgatca aggtcctgcg
     4981 cacgcgagga gcaatcaagg tcccgcgcac gcgaggagcg atcaaggtcc tgcgcacgcg
     5041 aggagcaatc aaagtcccgg cctggtggtg gtcgacaagc ccgccggcat gacgagccac
     5101 gatgtggttt cccggtgcag gcgcatcttc tccacccgca aggtcgggca tgccggcaca
     5161 ctggacccga tggccactgg tgtgctggtt gtcggcatcg agcgcgccac caagattctg
     5221 ggcctgttga tcgcgaccga caagtcctat gccgcaacga tccggctggg tcagacgacc
     5281 accaccgagg acgccgacgg cgaagtgctg cagacggttt cggccgcgaa cgtgaccgac
     5341 accgacatcg aagccgcggt cgacgcgctg cgtggcgaca tcgcgcagat cccgtcggcc
     5401 gtcagcgcga tcaaggtcgg cggtaagcgc gcctaccaac tcgcccgcga aggccagacc
     5461 gtcgagttgg ccgccaggcc ggtgcgcatc gaccgcttcg cggtgtgcga cgtgcggcgg
     5521 gcggctgacc tgatcgacgt cgacgtcgag gtggattgct cgagcggcac ctacatccgg
     5581 gcgctggcgc gcgatgtcgg ggccgcgctg aaggtcggcg ggcacctcac agcgttgcgc
     5641 cgaacccgcg tcggtcgctt cgggctcgac gaggcgcgcc ccctcgacga gttggctgag
     5701 aagccggcgc tgagctactc gctcgacgag gcctgcctgc tggccttccc gcgtcgcgac
     5761 ctgaccgccg acgaagccga gtccgcccgt cacggccggg cgttgcaccc cgccggcatc
     5821 gacggcgtgt atgcggcgag ggattcggac ggacatgtca tctcgctgct gcgagacgag
     5881 gccgcgaagg caaagtctgt ggtggttctg cgtcccgcga cgttgtaaaa gccctgcgga
     5941 gtgctgtccg tgtgacattc tgtgggcgtg gctacggccg cggtgagcgg gctattgccg
     6001 acggggatgg cgacgctgct tctggcagac gtcgagggct ccacgcggct gtgggagacc
     6061 cagcccgagg agatgagggc cgccctcgcg cagctcaacc acaccgtctc cgaagtcatc
     6121 gccgcctatg gcggggtgcg gccggtcgaa cagggcgagg gcgacagctt cgtggcggcg
     6181 ttcactcgcg cgtcggacgc cgtggcctgt gcagtgcagt tgcagcgggc accacttgac
     6241 ccgatccggc tgcgcatcgg gatgcatacc ggcgaagtgc agttgcgtga tgaaggcaac
     6301 tacgccggct cgaccatcaa ccgcaccgcg cggctgcgtg atctcgccca tggcggacag
     6361 acggtgctgt cgggcgcgac cgaggacatg gtcgtcgacc ggctcccggc cggcgcctgg
     6421 ctggccgatt tggggacaca ccagctgcga gacttacccc gcccagagcg cgtgatgcag
     6481 ctgtgtcacc ccgatcttga acgtgagttc ccgccattgc gtaccgctaa aagagttggg
     6541 gtgcacaatc ttccggttca actcaccaat ttcgtcggcc gcgaagcaga aatcgatgat
     6601 gtgcgtcggc tgctggtcga taaccggttg gtggtactga cgggagccgg tggcgcggga
     6661 aagacccggc tcgcggttga gatcgcggct cgcatcgcgc cggagttcgg tgacggcgtc
     6721 tggtatgtcg acctggcacc gatcacccat ccggcggtag tgccggtgac ggtggcgcgt
     6781 gcgctgggac tgcccgacca acccggccgc tccacgatgg atacgctgct gcggttcgtc
     6841 cgtgacaggc ccatgctggt ggtgctggac aactgcgagc acctcttgga cgccgccgcc
     6901 gagctggtcg tggctcttct cggtgaggca ccggggcttg ccgtgctcgc caccagccgt
     6961 gagccgatcg gggtggcggg tgaggcaacg tggcgggtgc catcgctctc agtgaacgac
     7021 gccgccatcg cattgttcgc cgaccgcgcc cgcttggccc agaccggctt cgcgatcaac
     7081 agtgaaaact cagcggcggt ggcggagatc tgcggtcgtc tcgacgggat gccgctggcc
     7141 atcgagctgg cggcagcgcg ggtgcgggcg ttgtcgctgg ccgaaatcct cgacggctta
     7201 cacgaccgct tccggctgtt gacaggtggt tcacgcaccg cggtacgccg tcagcagacg
     7261 ctgcgcgcct cagtggactg gtcgcatgcg ctcctgaccg aaccggagcg ggtcttgttc
     7321 cgccggctgg cggcgttcct ggcaggcttt gatctcgatg ccgcacaggc tgtcgtgggc
     7381 gatggcgacg tgcagcgcta ccaggtgctc gatcagctca ccctgctcgt ggacaagtcg
     7441 cttgtcgtca ccgaaaacag cagtggccga acgcgttacc gcatgctcga aacggtacgt
     7501 caatacgcgc tggagaaact ggctgagtcg ggcgaggccg atgtcgtccg cgcgcgtcac
     7561 cgcgaccact acaccgcgat ggccgccctg ctcgacgcgc cggcgcgcac cgactacgaa
     7621 cggcgtgtcg agcaggcaga ggaggagatg gacaatctgc gcgccgcttt cgtgtggagc
     7681 ctggaaaccg gcgacgtcgg tcgttcgttg gagctggcga catcgttgca gccgctgtgg
     7741 ttctccaggg ggcgcatccg ggaaggcctg gcctggttcg acgccgcctt caacggcgga
     7801 aacgagcgtc atgtcgacgt gccggacgca gtgcgcgcag gtgcgctcgc cgacaaagcg
     7861 gtgctcgaag catgggtggc cgccaagttc agcctggatc aagccgaaca agccctggcg
     7921 atcgcgcgcg aggtggaaga tcctgctctt gtgacgcgca cgctgaccgc ctgcggcgcc
     7981 gtcgccggct accacgaccg cgaggtggcc cagtcctact tcaccgaggc aatcggcctg
     8041 gcccgcgcga taggcgacca gtggagattg agccagatcc ttgccttgga ggcgcagggc
     8101 gccaccatag cgggtgaccc gctggcggcg cgcgcggcgg ccgaagaagg acgcgatctc
     8161 gccgatgcga tcggcgacgg gtccaactcg cgtcagtgcc gcttctggct cgggttggcg
     8221 cagggggcaa atggcgatct ggccggggcc gacaaacaat taagcgaggt gacagccgag
     8281 tccgcggcgg ctcacgatga gatctggagg ttgagcagtg tcgggagccg gggtgtcata
     8341 ctcgcctacc agggccacgc ggccggcgcc cgagcggctg ccgaggcgac cctcgagggt
     8401 ggagcggaaa tcggcgacgt gttggtcggg atgggctacc acgtgttgac ccttgccgcg
     8461 ctggctgccg gcgacggtgc gggggcccaa gaggcgggtg aggccgcgtg gaagcacatc
     8521 agcgttgcgg gcgagatggg cgcgatgtgg cgctactggg gcggcgaggc ggcgttggcg
     8581 ggcggggatc tcgccgtcgc gcgctgctgg gcggacgagg ccgtctcggc aacgaggggc
     8641 atctacttgt cgtgggcgct gacggggcgc gctcgcgttc tgatggcgga gggtcagtca
     8701 aggcaggccg agagcgacgc ccacgatgcg ttggcgcggg ccgctgaaaa ccaggcgcga
     8761 atctgtgttc ccgacgttct cgagatcctc gccgacctgg cgggaaaatc cggtcgtcgc
     8821 cctttagctg cgcggctttt cggcgcggca cagggcatca ggcaacggat cggcgtcgtc
     8881 cgtttcaaga tctacgacaa cggctatcag acctcggtaa gctcgctgcg agatgcgatg
     8941 gccgagaagg acttcgacgc cgcatgggct gaaggcgcag ccctgtccac tgaggaagcc
     9001 atcgcctacg cccagtccgt tcgccgtggc tgacccgggg gaactcaggt cggctgctag
     9061 ctgaacgccg tttcgaagcg gacgcgcggg catttgtcag ctcaaactga taagcctagc
     9121 ctgatgacat gcccgtcgac cgccagaccg aagccgtggc gagggccctg ctcttgtgcg
     9181 tcgggctgct acgccgccgg ctgcgtcaga cgcccgtcac cggcgagctg accttcccgg
     9241 aaaccgcagc gctcggccgt ctggaccgtg gcggcccggc ggccgcggcc gacctcgcca
     9301 gacaggagca gatcagcccg cagtcgatgg gcgccacgct ggccgagctg gaagcccgcg
     9361 gcctggtcaa gcgccaaccc gatcccggcg acgggcggcg aatcctgttg tccatcagcg
     9421 ccgcaggccg caaagagctc aaccgcaggc gcaacgcccg cgtcgagcaa ctgaccgcgg
     9481 gcctggccga tttcaccgaa gccgaactcg agcaactcgc ggccacggca cccctgatcg
     9541 aacgactggc gtaccacctg tagatgtctg tcgacgaaca agaaaccccg acccgcgaca
     9601 actaccgctg ggtcgcgctg accaatacca ccgcggccgt cttcatgtcc gcactcgacg
     9661 ggtcgatcgt catcatctcg ctgcccgcga tcttccgcgg catccacctg gatccactgg
     9721 cgccggccaa catcacctac ctgctgtgga tgatcatggg ctaccggctg gtgcagtccg
     9781 tgctcgtcgt aacgctgggc cgcatcggcg acatgtacgg ccgggtgagg gtctacaacg
     9841 cgggcttcac cgttttcacc gtcgcgtcga tcctgttgtc gttcgacccc tttgacggct
     9901 cggccgcagc ccaatggctg atcggctggc gcctgctgca ggcggtcggg ggatcgatgc
     9961 tgaccgcgaa ctccgcggcg attctcaccg acgccttccc acccgaccgg cgcggctttg
    10021 cgctcggctt caaccaggtc gccgcgctgg ccgggcagtt catcgggctg gtggccggtg
    10081 gcctgctcgc cgcgtgggat tggcgggcgg tgttctgggt gaacgtgccc gtcggggtgt
    10141 tcggcacgat atgggcctat cga
//
LOCUS       DASYLX010000469         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3641967,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000469 DASYLX010000000
VERSION     DASYLX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641967"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /gene="uppS"
                     /locus_tag="VGP27_11405"
     CDS             <1..446
                     /gene="uppS"
                     /locus_tag="VGP27_11405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV7851948.1"
                     /translation="LNDMGVRMRWVGSRPRMWRSVIKEFDIAEQMTVGNDVITINYCV
                     NYGGRTELVEAARELAQEAVDGKVNPNRISEAAFARHLHRSDIPDVDLFIRTSGEQRA
                     SNFLLWQAAYAEYVFQDKLWPDYDRRDLWEACEEYVNRNRRFGRA"
     gene            443..850
                     /locus_tag="VGP27_11410"
     CDS             443..850
                     /locus_tag="VGP27_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003906807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851949.1"
                     /translation="MMDLADRVRAVLDGVLRYEEEDDGALTVSYEHTFTSLRVVAIAE
                     DLEMVSLTQILAWNVPLDNEFRDKVAQLTHDTVLGTVSLAEQVAGSGVADVTLRYNFP
                     AAGLTDEALRTLIIMVISAGADVRETLSGDADS"
     gene            complement(847..1230)
                     /locus_tag="VGP27_11415"
     CDS             complement(847..1230)
                     /locus_tag="VGP27_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV7851950.1"
                     /translation="MRATRQRAAISALLENLDDFRSAQELHDELRRRGEGTGLTTVYR
                     TLQSMAAAGLVDTLRTDTGESLYRRCSDHHHHHLVCRVCGCTVEVQGGQVETWAADVA
                     REHGFSDVSHTIEIFGVCGGCAQAD"
     gene            complement(1349..1726)
                     /locus_tag="VGP27_11420"
     CDS             complement(1349..1726)
                     /locus_tag="VGP27_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7851951.1"
                     /translation="MKTVSTFSSGLAPDTSDHHQHSDAPVPDLPSREVLDTAGDLLRA
                     LAAPVRIAIVLQLRESSRCVHELVDALGVPQPLVSQHLRILKSAGVVAGERSGREVLY
                     RLVDHHLAHIVVAAVTHAAEDSE"
     gene            1798..>3188
                     /locus_tag="VGP27_11425"
     CDS             1798..>3188
                     /locus_tag="VGP27_11425"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV7851952.1"
                     /translation="MRVNVATNASLRCSPVASIIDTVANLAKRRGLVYQSGEIYGGTK
                     SAWDYGPLGVELKENIKRQWWRSVVTSRDDVVGLDSAIILPREVWVASGHVEVFNDPL
                     VECLNCHKRHRQDHLQEAYAEKKGIEDPESVPMTEIVCPDCGTKGQWTEPRDFNMMLK
                     TYLGPIETEVGLHYLRPETAQGIFVNFANVVTTSRKKPPFGIGQIGKSFRNEITPGNF
                     IFRTREFEQMEMEFFVEPDSAPEWHQYWIDNRLQWYVDLGIQRDNLRLFEHPKDKLSH
                     YSDRTVDIEYKFGFQGNPWGELEGVANRTNFDLSTHSAHSGVDLSFYDQATDTRYVPY
                     VIEPAAGLTRSFMAFLVDAYAEDEAPNAKGGVDKRTVLRLDPRLAPVKAAVLPLSRNE
                     DLSPKARDLAAELRKSWNVEFDDAGAIGRRYRRQDEIGTPFCVTVDFDTLDDQAVTIR
                     ERDAMSQERIALDA"
BASE COUNT          586 a         1026 c         1042 g          534 t
ORIGIN      
        1 acctcaacga catgggcgtg cggatgcgct gggtgggttc gcggccgcgg atgtggcgca
       61 gcgtcatcaa agagttcgac atcgccgagc agatgacggt cggcaacgac gtcatcacca
      121 tcaactactg cgtcaactac gggggccgca ccgaactcgt cgaggcggcg cgcgaactgg
      181 ctcaggaagc cgtcgacggc aaggtcaacc ccaaccggat cagcgaggca gcgttcgccc
      241 gacaccttca ccggtccgac atccccgacg tcgacctgtt catcagaaca tcgggggagc
      301 agcgcgcgag caacttcctg ctgtggcagg ccgcgtacgc cgaatatgtc tttcaggaca
      361 agctgtggcc ggactacgat cgccgcgacc tgtgggaggc gtgcgaggag tacgtcaacc
      421 gcaaccgaag gttcggcagg gcctgatgga cctcgccgat cgggtgcggg ccgtcctcga
      481 cggcgtgctg cgctacgagg aagaagacga cggcgcgctc accgtttcct atgaacacac
      541 cttcacgtcg ctgcgggtgg tggcgatcgc cgaggatctc gagatggtgt cgctgaccca
      601 gatcctcgcg tggaacgtac cgttggacaa cgagtttcgt gacaaggtcg ctcagctgac
      661 gcacgacacc gtgctgggca cggtgtcact ggccgagcag gtggccggga gtggcgtcgc
      721 cgacgtcact ctgcgctaca acttcccggc cgcgggcctg accgatgagg cgctacggac
      781 gctgatcatc atggtgatca gtgcgggcgc cgatgtccgt gagacgttga gcggcgacgc
      841 cgactcctaa tcggcttgtg cgcagccgcc gcagacgccg aagatctcga tcgtgtggct
      901 gacgtcggag aagccgtgct cgcgtgcgac gtcggccgcc catgtctcca cctggccgcc
      961 ctgcacctcg accgtgcaac cgcacacccg gcacaccaaa tggtggtggt gatggtccga
     1021 gcaccgtcgg tacagcgact cgccggtgtc ggtgcgcagg gtgtccacca ggccggccgc
     1081 cgccatcgac tgcagggttc ggtaaaccgt cgtcaggccg gtgccctcac cacggcggcg
     1141 cagttcgtcg tgtagctcct gggcggaacg gaagtcgtcg agattctcca gcaatgccga
     1201 gatcgccgcg cgctgcctgg tggcgcgcaa tccgggcggc gtgcccgaat tgctcgcgtg
     1261 cgcggtgcct gaatcgctcc tcgcgtccgc ggtgctcact cgctcctcgc gtccgcggtg
     1321 gctgaatcgc tcctcgcgtc cgcggtgctc actcgctgtc ctccgcggcg tgcgtcacgg
     1381 ccgcgaccac gatgtgcgcg aggtggtgat cgacgaggcg gtagagcacc tcgcggcctg
     1441 agcgctcgcc ggccacgaca ccggcggatt tgagaatgcg caggtgctgg ctgaccagcg
     1501 gttgcggcac gccgagggcg tcgacgagtt cgtgcacgca gcgcgacgac tcgcgcagct
     1561 gcaacacgat cgctatccgc accggcgcgg cgagtgcgcg cagcaggtca cccgccgtgt
     1621 cgaggacctc gcgagacggc aggtccggca ccggggcgtc gctgtgctgg tggtgatcag
     1681 aagtatccgg cgccagcccc gatgagaagg tggaaaccgt tttcattatg tccgccacga
     1741 tacatgcgtg atcacgcatg tcaacggcgt cgtgtttcta gcgtttgatg gggcgaagtg
     1801 cgagttaacg tcgcaacaaa tgcatcgtta cgctgttcac ccgtggcctc catcatcgac
     1861 accgttgcaa acctggccaa gcgccgaggc ctggtctatc agtccggcga aatctatggc
     1921 ggcaccaagt cggcttggga ttacggccca ttgggggtcg agctcaagga gaacatcaag
     1981 cggcagtggt ggcgttccgt ggtcaccagc cgcgacgacg tcgtcggcct cgacagcgcg
     2041 atcatcctgc cgcgcgaggt ctgggtcgcc tccggccacg tcgaggtgtt caacgacccg
     2101 ctggtggaat gcctgaactg ccacaagcgg catcggcagg accatctgca ggaggcgtac
     2161 gcggagaaga aggggatcga agatcccgaa tccgtcccga tgacggagat cgtgtgcccg
     2221 gactgcggga ccaaagggca gtggaccgag ccgcgcgact tcaacatgat gctcaagact
     2281 tacctcgggc cgatcgagac cgaggttggc ctgcactatc tgcggccgga gaccgcgcag
     2341 ggcatcttcg tcaacttcgc caacgtcgtc acgacgtcac gtaagaagcc gccgttcggc
     2401 atcggccaga tcgggaagag tttccgcaac gagatcaccc ccggcaactt catcttccgc
     2461 acccgcgagt tcgagcagat ggagatggag ttcttcgtcg aaccggacag tgcacccgaa
     2521 tggcaccaat actggatcga caacaggctg cagtggtacg tcgacctcgg tatccaacgc
     2581 gacaatctgc ggctgttcga gcaccccaaa gacaagctgt cgcactactc ggaccgcacc
     2641 gtcgacatcg agtacaagtt cggcttccag ggcaacccgt ggggcgagct ggagggcgtc
     2701 gccaatcgga ccaacttcga cctgtcaacg cactcagcac attccggtgt cgacctgtcg
     2761 ttctatgacc aggccaccga cacccggtat gtgccgtacg tcatcgaacc ggcagcgggc
     2821 ctgacgcggt cgttcatggc gttcctcgtc gacgcctacg ccgaggatga ggcacccaat
     2881 gccaagggtg gtgtcgacaa gcgcacggtg ctgcgccttg atccacgcct ggcaccggtc
     2941 aaggcggccg tgctgccgct gtcgcggaac gaggacctgt cacccaaggc gcgtgatctc
     3001 gccgccgagt tgcgcaagtc ctggaacgtc gaattcgacg acgcgggcgc catcggccga
     3061 cgctatcgcc gtcaggacga aatcggcacg ccattctgtg tgacggtcga cttcgacacg
     3121 ctcgacgacc aggccgtcac gatccgggag cgcgacgcga tgtcgcagga gcggatcgcg
     3181 ctcgacgc
//
LOCUS       DASYLX010000470         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1337632,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000470 DASYLX010000000
VERSION     DASYLX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337632"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VGP27_11430"
     CDS             <1..629
                     /locus_tag="VGP27_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7851953.1"
                     /translation="SAVYDILGFGKPPEQVPFAATPGVFTFGWGPVLHQLADGLGVGV
                     DDIKETVERIPADISFDTPTGHIAAGTIAAMRSTLTGYVEGKPTLVIDHVSRMHDDIA
                     QDWPQPHISIQPRDLGYGGASGRGVYRVEIEGSPAMRCEFEMADDHDHDLGARMAGAS
                     RMVNAIPAVCAAKPGLLSALDLPLITGKGLVKRVSGPSPDSRLVGISS"
     gene            716..1714
                     /locus_tag="VGP27_11435"
     CDS             716..1714
                     /locus_tag="VGP27_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851954.1"
                     /translation="MSSPGSARVDFHHHLFPGGELADFTQRSLIANSGWRFPGGAPHW
                     TPETSLAFMDSLGIQTAVLSLPSDLELGLPAQERREFAREVNTIARQTVDDHPGRFGF
                     FAHLPTPTDVDAALDELTYALDHLDADGITLTNVYGTGEEARSLGDDIFDPLWAELDR
                     RGTLVFLHGEQTPGRNRMPNRFLPTPISEVPNETYKAAADLVTSGRKRQYPNVRIILS
                     HSGGSTPFLAARVAVLSHYLGCELSPEEIIADFRTFYFETALSGFETNLVALENFVPP
                     DRILFGTDFPAVSPQMAGWYTTNVDNYFADRPDMLAQVMHGNAQTLLPRLAANAAA"
     gene            1747..2694
                     /locus_tag="VGP27_11440"
     CDS             1747..2694
                     /locus_tag="VGP27_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851955.1"
                     /translation="MTLIALEEHVLPAELINEVWPDSRGSESLTAKLADIGEQRLPAM
                     DEAGIEMQVLSVVAPGSQQVPAEPAVHLSRALNDRCAEIVAAHPIRFSALATLPTQDS
                     DAAIVEAKRAITELGFCGVVINGHTHGRFLDAPEFDELLTAIEELDVPIYLHPTYPPP
                     EVAGVYFGHLPESTGRALATAAWGWHAETGLHILRLAATGVFSRHPKLQIVVGHMGEN
                     LPFSLMRADSILTRANPDSPSVAQAIREHVHITICGYTTTPPLLCALQVFGADRIMFS
                     SDYPFGDPVQHAKFLAHAPISPADRDKIAYRNAQHLFRL"
     gene            complement(2719..>4023)
                     /locus_tag="VGP27_11445"
     CDS             complement(2719..>4023)
                     /locus_tag="VGP27_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV7851956.1"
                     /translation="PEYFRGGPVPVRGWNSVTVPGAVSAWTELHARFGKLPFARVFEP
                     AIGYGRKGFLVSPTVAVQWAGQVPKLRSQPGFAEAFLPDGRPPAPGERFRLPDHAATL
                     ETIAATDGEAFYRGALAATMEAHSAANGGAMMASDLAAHRAEWVDPIGIDYRGYTLHE
                     IPPNGQGIIALISLGILRNFDMASLPVDSADSVHLQIEAIKLAFADALAYVADIDHMP
                     VRSNQLLDADYLRQRSTLIDPVRAKPVSAGRPPKGGTVYLTAADADGMMVSMIQSNYD
                     GFGSGVVVPGTGISLQNRGTEFSATPGHPNLVGPRKRPYHTIVPAFLTRNGAPVMSFG
                     VMGGTMQPQGHMQLMVRIADHGQNPQAACDGPRFRWLQGMQVSLEDGFPPATVDELRR
                     RGHDVVAVDDYNQFGSCQAIWRLPDGYLAASDPRRDGQAAGF"
BASE COUNT          725 a         1424 c         1212 g          662 t
ORIGIN      
        1 cgtccgccgt gtatgacatc ctcggattcg gcaagccgcc agagcaggtc ccgttcgccg
       61 cgacaccggg tgtgttcacg ttcggctggg ggccggtgct gcaccagttg gccgacggcc
      121 tcggggttgg cgtcgacgac atcaaggaaa ccgtcgagcg catacccgcg gacatctcgt
      181 tcgacacccc gaccgggcac atcgccgccg gaaccatcgc cgcgatgaga tccacgttga
      241 ccgggtatgt cgagggcaaa ccgaccctgg tcatcgacca cgtcagccgc atgcacgacg
      301 acatcgcgca ggactggccg caaccacaca tatcgatcca gccgcgcgat ctcggttacg
      361 gcggagcctc cggccgcggc gtctaccgtg tcgagatcga gggttcaccg gccatgcggt
      421 gcgaattcga aatggcagac gaccacgatc acgatctagg cgcgaggatg gccggcgcgt
      481 cccggatggt caacgcgatt ccggcggtct gcgccgcgaa accgggtctc ctttcggcgt
      541 tggacctgcc gctgatcaca ggcaagggat tggtgaagcg ggtgtccggc ccgtcgccgg
      601 acagccggct ggtcggaatc tcgagctgaa gcgcggccac tccgggcagg accaacagtt
      661 gccagctagc cgcaattcac acctggcaat aaatggcttc actggtggta tgaccatgag
      721 cagtccaggg tccgcacggg tcgatttcca ccatcatctc ttcccgggtg gcgagcttgc
      781 tgacttcaca cagcgcagcc tcatcgcgaa ctccggctgg cggttccccg gcggcgcccc
      841 gcactggaca ccggaaacca gcctcgcctt catggacagc cttggtattc agaccgccgt
      901 gctgtctctg ccgagcgatc tggaattggg cttgcccgcg caggagcgtc gggaattcgc
      961 ccgcgaggtc aacaccatcg cccgccagac ggtcgacgac caccccggcc ggttcggctt
     1021 cttcgcgcac ctgcccactc ccaccgacgt cgacgccgca ctcgacgagc ttacgtacgc
     1081 ccttgatcac ctcgacgccg acggcatcac cctgaccaac gtctacggca ccggcgaaga
     1141 ggcccgatcg ctcggcgacg acattttcga cccgctctgg gccgaactcg accggcgcgg
     1201 cacgcttgtc tttctccacg gcgagcagac accgggtcgc aaccggatgc cgaatcggtt
     1261 cctgcccacg cccataagcg aggtgccgaa cgaaacctac aaggctgcag cggatctcgt
     1321 caccagcggc cggaaacggc agtatccgaa cgtgcggatc atcctttcgc actccggcgg
     1381 aagtacacca ttcctcgccg cacgggtcgc ggtgctctcc cattacctcg gctgtgagct
     1441 cagtcccgag gagatcatcg ccgacttccg caccttctac ttcgagacgg cgttgtcggg
     1501 cttcgagacg aatctcgtcg cgctggagaa cttcgtccct cccgaccgga tcctgttcgg
     1561 cacggacttc cccgcggtca gtccacaaat ggccggctgg tacacgacca acgtcgacaa
     1621 ctacttcgca gaccggcccg acatgctcgc gcaggtaatg cacggcaatg cgcagacact
     1681 cctgccccga ctggctgcga acgccgcggc ctgagtcgcc gcagcgccga cggcgaaagg
     1741 atcgtcatga cactgatcgc ccttgaagag cacgtgcttc cagccgaact catcaacgag
     1801 gtgtggcccg actcgagggg atccgagtcg cttaccgcga aactggcgga catcggcgag
     1861 cagcggctgc ccgctatgga cgaagcgggc atcgaaatgc aggtgctgtc ggtcgtggcg
     1921 ccaggatccc aacaggtccc tgccgagccc gcagtccacc tcagtagagc gctcaatgac
     1981 cgctgcgccg aaatcgttgc ggcgcatccc atccggttca gcgcattggc caccctgccc
     2041 acccaagact cggacgccgc gatcgtcgaa gccaagaggg caatcaccga gctgggattc
     2101 tgcggggtgg tcatcaacgg gcacacccac ggacggttcc tggacgcacc cgagttcgat
     2161 gaattactca cggccatcga ggagctcgac gtgccgatct atctgcaccc cacctatccc
     2221 ccgccggagg tcgccggcgt ctacttcggt caccttccgg aatcaaccgg cagggccttg
     2281 gccaccgcgg cctggggctg gcatgccgaa acgggtctgc acatattgcg actcgcagca
     2341 accggcgtct tcagccgaca tcccaagttg cagatcgttg tcggacacat gggcgagaac
     2401 ctgccgttct ccctaatgcg cgcagactcc attctcaccc gcgccaaccc ggacagcccg
     2461 tcagtggcgc aggcgatccg cgaacacgta cacatcacca tctgcggcta caccacgaca
     2521 ccgccactgc tgtgcgcgtt gcaggtgttc ggcgccgacc gcatcatgtt ctccagcgac
     2581 taccccttcg gcgacccggt tcagcacgca aagttcctag cccacgcccc gatcagtccg
     2641 gccgaccggg acaaaatcgc ataccgcaat gcccaacacc tcttccggct ctgaacacgg
     2701 ccggggccgg gatcagagct agaaaccggc ggcctgcccg tcgcgccgcg ggtcgcttgc
     2761 tgcgagatac ccgtccggca accgccagat cgcctggcag ctgccgaact ggttgtagtc
     2821 gtcgaccgcg acgacgtcgt ggccgcggcg gcgtagctcg tcgaccgtcg ccggcgggaa
     2881 gccgtcttcg aggctcacct gcatgccttg tagccaacgg aacctcggac cgtcacacgc
     2941 cgcctggggg ttctgaccgt gatcggcgat gcggaccatc agctgcatgt gcccttgtgg
     3001 ctgcatggtg ccgcccatca cgccgaagct catcaccggt gcgccgtttc tggtaaggaa
     3061 ggcgggaacg atggtgtggt acgggcgctt tcgcggccca acgaggttcg ggtgccccgg
     3121 ggtcgcgctg aactccgtgc cgcggttctg caacgagatc cctgtgccag gcaccactac
     3181 gcccgacccg aagccgtcgt agttggactg gatcatcgac accatcatgc cgtcggcgtc
     3241 ggccgcggtc agatacaccg tgccgccctt cggcggcctg cctgccgaaa ccggtttggc
     3301 ccgcaccggg tctatcaacg tcgaccgctg cctgaggtag tcggcgtcca gcagctgatt
     3361 cgaccgcacc ggcatgtgat cgatgtcggc cacgtaggcc agcgcgtcgg cgaatgcaag
     3421 cttgatcgcc tcgatctgta ggtgcacgct gtctgccgaa tccactggga gcgaagccat
     3481 gtcgaagttc cgcagaatgc caagagagat caacgcaatg atcccctgcc cgttcggtgg
     3541 aatctcgtgc agcgtgtagc cgcggtagtc gatgcctatc ggatcgaccc actccgcgcg
     3601 gtgagcggcc aagtcggagg ccatcattgc gccaccgttc gcggctgaat gcgcctccat
     3661 cgtcgccgca agcgcgcccc ggtaaaacgc ctccccgtcc gtcgccgcaa tcgtttccaa
     3721 cgtcgccgcg tggtcgggaa gccggaaccg ctcccccggc gccggcggcc gcccatccgg
     3781 aagaaacgcc tcggcaaacc ccggctgcga tcgcagtttc ggcacctgcc cggcccactg
     3841 cacggccacc gtcggcgaga cgaggaaccc cttgcgtccg tagccgatcg ccggctcgaa
     3901 gacccgcgcg aacggtagct tgccgaaccg ggcgtgtagt tcggtccacg ccgacaccgc
     3961 gccgggcacg gtgaccgagt tccagccccg caccggcacc gggccgccgc ggaaatactc
     4021 cgg
//
LOCUS       DASYLX010000471        11331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3752914,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000471 DASYLX010000000
VERSION     DASYLX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3752914"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VGP27_11450"
     CDS             complement(<1..94)
                     /locus_tag="VGP27_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV7851957.1"
                     /translation="MVWRYIKAQLMVLLCGGLVGPIFLAVYFATG"
     gene            complement(163..999)
                     /locus_tag="VGP27_11455"
     CDS             complement(163..999)
                     /locus_tag="VGP27_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7851958.1"
                     /translation="MFCRTSAAACAVAVAGSALVGLTTGTANAAPGCPDVHWMGAAGS
                     GERTEYDMAANGGMGQVVYQSYQALQQQLAQEGRTITAEAVDYPAKDVPEDAGVGDWL
                     DYMGSVDAGTAALGAQYAAFTAQCPTTKVVLAGYSQGAMVVHRNLVAIGADPNLAASL
                     LIADGDRITDDTTVNVGSASKDPQKTGGASQDWPILAHAPAELPANVGVRTISVCDAG
                     DAVCDYDPDAAEVSATAVAIHTSYTVGGANGQMWTWMVHQLLGSSSADAPQVPAVATS
                     RT"
     gene            1130..2509
                     /locus_tag="VGP27_11460"
     CDS             1130..2509
                     /locus_tag="VGP27_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7851959.1"
                     /translation="MNSPSSTVRSRWILAAVSTALFCVQIDYFAMNLALPRMASDLNS
                     NATDLQWVISVYMLALGAFMVPAGRIGDIFGRRRALLAGIALFGLASALCAVAPSAGL
                     VITFRAVQGVGAALIFPVSVSVLTNAFPAARAGHAIGLAYGVAGLGNAAGPLVGGLLT
                     QTLGWRSIFWLLVPFSLAAFVIGALTVPESSDDTVPRRIDFAGLSLIIFGIGLFTLTF
                     DRAPSRGWLAPGTLVAFVGSLAVVGLFLLVEGRVRWPLVDLSLFRNARFTILVVAGTV
                     ANIAYAVTIYLSTLNLQQVRGLDPLMAGLAFLGPSIGAALGGPLSGRLATRYPPVLVM
                     GLGCVAAALSLATLAISSGRVVYVIALSACGFTMGLVYAFTTVATQAVVRPERAGEAA
                     GVALTSLVTLAGVGVAVSGTMLEVLRQGGMPTASGIPAIFLAIAAVLLPAAIAVLAFR
                     KHEGTLDHR"
     gene            complement(2559..4871)
                     /locus_tag="VGP27_11465"
     CDS             complement(2559..4871)
                     /locus_tag="VGP27_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851960.1"
                     /translation="MGKHRKTRASHTPAKLNRTCAICAVAGATGFGLISFAPMAFADN
                     DSNDGWWIGSGNNTPIGNNGNGNTSQNGAFNGNVMNNQFNGLSPIIGGTAVNAGAATG
                     GAGTSAATNAGGAIGGAAVGTTVSANPAVGGTGAGGAAVGGQNVAANTGGLAAGGSNI
                     GTATSGVATGGQVLDGPDIAGQGLGGAGTSLGGAGGAATGGTNVSAGNIGGAATSAAG
                     GGTASEVIVQGNTTGDLGLLSPDAPPSPDNSIGGAVAGPGVVGATAGGNAITGQASGG
                     GTGGAGEGGAGAGGDGIGGGILQDQEGGEAVVGGNAIQQSATGGEAGAIANGQQAGNA
                     NGGYGVGGNATSGNTTGGTTVGGAAVNNSVAAAGDATGGDATNEVIGLAAGNLATNVV
                     NSNTNTGNGNDSDNSVGTLGSNSSDDGWWIGSGNNTTIGNNGNGNSSQNGAFNGNIMN
                     NQMNVLSPVVGGTAVNGAAATGGIGTSAATNAGVATAGAAAGTTSSVNPALGGEAIGG
                     VAAGGTNGAVNTGSSATGGTNVGAGTTGAAVGGSVVDAADTAGSALGGAGTGGLGLGG
                     AGTGGINTAAANAGGPAVGAALGGITTEAVTQINTTLAGDPDNSVGGATAFGGQAGPT
                     TGGSAVTGQLAGGGTGGDGFGGEGTGGNAAGGSIVQAQTGGAATVDPNTVNQGATGGT
                     GGTLTNSQIAGAATGGNSAGGNATSGSTVGGNTTGGAATVNQSTTSGTATGGTATNRA
                     SGTASGNSSHNTSNTNTNSNNGNGSSSSVG"
     gene            complement(4998..6221)
                     /locus_tag="VGP27_11470"
     CDS             complement(4998..6221)
                     /locus_tag="VGP27_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851961.1"
                     /translation="MLVRAIGAGGAAACIGLLAEINAPDAEALSILLPAGAGNATQIN
                     ILEGNVFDPQFGLGGNGSNTSHNSTIGALFFGQGNGGKDTSDGGIFGPIALGGATGNG
                     NVTQVNILSYNIFNPQGSLMGGNTSDNTTISNVAVNNGNGATTTSEGTSTFVGGAVGN
                     GNTTQLAFFSGNIFNPQFSLFGGNVSDNTAITNVAAGNGTGSNTSVTSGGLFGTSLFG
                     LTGNGNTNQTAVGTSNIINPQFSLLGSNLSYNYANANQASGNGGGSGNNVGSTGGLGN
                     IFAVGDTGNGNTTQNATGSGNIYNDQLNLGLGGFIPSLTPPTTSQAVQEPLTNEQVTS
                     ATSGTQPGLTNRELLDLQNQQAGTTSSTGTVVGPRPLNDAVTRIKDAVGDTVDRITKP
                     LKPKAEIKPDQPGAS"
     gene            6758..7762
                     /locus_tag="VGP27_11475"
     CDS             6758..7762
                     /locus_tag="VGP27_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEV7851962.1"
                     /translation="MKIARIPAANEKFELTEVEIPEPDPGHVRVKVHACGVCHSDSLT
                     VAGALGNSFPRAPGHEIAGVVDAVGDGVTTWKPGDRVGVGWFGGCDFTCEPCRRGDFI
                     SCENGRIPGIAYDGGYAEYMVAPAEAIARIPDDLADVDAAPLMCAGITTFNALRESVA
                     RPGDVVAILGVGGLGHLGIQFAARMGFEVVAIARGTDKEPLARELGAHHYIDSRASDV
                     AEELNKLGGAQVVLATVTVADAMTVTLNGLKARGQLVVVGAPNDPLQVPGAAIIFKSS
                     SVQGHASGTSQDSEDTLHFSALTGVRPMIETVPLSQAQAAYDKMMSGAARFRMVLTME
                     "
     gene            complement(7910..9100)
                     /gene="tuf"
                     /locus_tag="VGP27_11480"
     CDS             complement(7910..9100)
                     /gene="tuf"
                     /locus_tag="VGP27_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV7851963.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPELN
                     EASAFDQIDNAPEEKARGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADMVDDEELIELVELEVRELL
                     AAQEFDEDAPVIKVSALKALEGDPEWVKSVEELMDAVDESIPDPVRETDKPFLMPVED
                     VFTITGRGTVVTGRVERGVINVNEEVEIVGIKPGTTKTTVTGVEMFRKLLDQGQAGDN
                     VGLLVRGVKREDVERGQVVVKPGTTTPHTEFEGSVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVT
                     KIVT"
     gene            complement(9274..>11331)
                     /gene="fusA"
                     /locus_tag="VGP27_11485"
     CDS             complement(9274..>11331)
                     /gene="fusA"
                     /locus_tag="VGP27_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV7851964.1"
                     /translation="GIMAHIDAGKTTTTERILYYTGISYKIGEVHDGAATMDWMEQEQ
                     ERGITITSAATTTFWKDHQINLIDTPGHVDFTVEVERSLRVLDGAVAVFDGKEGVEPQ
                     SEQVWRQADKYEVPRICFVNKMDKVGADFYFSVKTMEDRLGANVIPIQLPIGSESEFE
                     GIVDLVEMNAKVWRGETKLGETYDTIEIPADLQERADEYRTKLLEAVAETDEALLEKY
                     FGGEELTVEEIKGALRKLTITSEAYLVLCGSAFKNKGVQPMLDAVIDYLPSPLDVPPA
                     TGHAPGKEDEIIIREPSTDEPFAALAFKVATHPFFGKLTYVRVYSGKVDSGSQVINAT
                     KGKKERLGKLFQMHSNKENPVEYASAGHIYAVIGLKDTTTGDTLSDPNQQVVLESMTF
                     PDPVIEVAIEPKTKSDQEKLSLSIQKLAEEDPTFKVHQDVETGQTVIGGMGELHLDIL
                     VDRMKREFRVEANVGKPQVAYKETIKRKVDKVEFTHKKQTGGSGQFAKVLISIEPFTG
                     EDGATYEFENKVTGGRIPREYIPSVDAGAQDAMQYGVLAGYPLVNLKVTLLDGAYHDV
                     DSSEMAFKIAGSQVLKKAAQQAQPAILEPIMAVEVTTPEDYMGEVIGDLNSRRGQIQA
                     MEERSGARVVRAHVPLSEMFGYVGDLRSKTQGRANYSMVFDSYAEVPAQVSKEIIAKA
                     TGQ"
BASE COUNT         1712 a         3668 c         3618 g         2333 t
ORIGIN      
        1 ggccggtggc gaagtacacg gccaaaaaga tcggaccgac gagaccgccg cacagcagca
       61 ccatcagctg agctttgatg taacgccaca ccatctgcca cgctcctcgc tcgacgcggt
      121 atgcgtgtca gattagcgcc agtaggcgcc tttgacgccg agttacgtgc ggctggtggc
      181 gacggcgggg acctgagggg catcggcgga cgatgatccg agcagttggt ggaccatcca
      241 cgtccacatc tgcccgttgg caccgccgac tgtgtagctc gtgtgaattg ccaccgctgt
      301 cgctgagacc tcggcggcat ccgggtcgta gtcacagacc gcatcgccgg cgtcacagac
      361 gctgatggtc cgaacaccga cattggctgg cagctcggct ggcgcatggg ccaagatcgg
      421 ccagtcctgc gatgcgccgc ccgtcttctg cgggtccttc gacgccgaac cgacgttcac
      481 tgtggtgtcg tccgtgatgc ggtcaccgtc ggcgatcaag agtgacgcgg ccagattggg
      541 atcggcgccg atcgcgacca ggttccggtg cacgaccatc gcgccctgag agtaaccagc
      601 aagcaccacc ttcgtggttg ggcattgtgc cgtgaacgcc gcgtactgcg cgcccaaggc
      661 tgccgtgccg gcatccacac tgcccatgta gtcaagccag tcccctactc cggcatcctc
      721 gggcacatcc ttggcggggt agtcgaccgc ttcggcggtg atggtgcgac cttcctgcgc
      781 gagttgctgc tgaagagcct ggtacgactg atagacgacc tgtcccatgc cgccgttggc
      841 cgccatgtcg tattcggtcc gctcacctga gccggcagca cccatccagt gaacgtcggg
      901 gcaaccgggc gccgcgtttg cggtaccggt cgtcaacccg acaagagcgc ttcctgccac
      961 agcgaccgca caagccgccg cactcgtccg acaaaacata aaacccccaa cccgacctgc
     1021 aaaaacactt taactgcgac ttttatcgcc acataggtct caggcgttac agaaacatca
     1081 caaaagcagg atcggcggta cggggcgttg cgggccgtat cgtcatcgcg tgaactcgcc
     1141 gagcagcacc gtgcgttccc gctggatctt ggcggcggtg tcgacggcgt tgttctgcgt
     1201 ccagatcgac tacttcgcga tgaatctggc gttgccgcgg atggcgtcag acctcaacag
     1261 caatgccact gatctgcagt gggtcatcag tgtctacatg ctggcgttgg gtgcgttcat
     1321 ggtgccggcg ggccggatcg gggacatctt cggccgacga cgggcgctac tggccggcat
     1381 tgcgctgttc gggcttgcat cggcgctatg cgcggttgcg ccgtcggccg ggctggtcat
     1441 cacctttcgc gccgtgcagg gcgtcggtgc ggcgctgata tttcccgtgt cggtcagcgt
     1501 gctgaccaat gcctttcccg ccgcgcgcgc cggccatgcg atcgggttgg cctacggtgt
     1561 cgcgggtcta ggcaatgcgg cggggccgtt ggtgggcggc ctgctgaccc agacgctggg
     1621 ctggcgatcg atattctggt tgcttgtgcc gttctcgttg gcggcgttcg taatcggcgc
     1681 tctcaccgtc ccagaaagtt cggatgacac cgtgccgcgc cgcatcgact tcgccgggct
     1741 gtcgttgatc atcttcggaa tcgggttgtt cacgttgacg tttgaccgcg cgccgagccg
     1801 gggctggttg gcgccgggga cgcttgtcgc gttcgtcggc tcgctcgcgg tggtgggctt
     1861 gttcctcctt gtggaggggc gggtgcgctg gccgctggtc gacctgtcgt tgtttcgcaa
     1921 cgcacgcttc acaatcctcg tcgtcgcggg caccgtggcc aacattgcgt acgccgtcac
     1981 catctacctc tccaccctga atctgcagca ggtccgaggc ctcgatccgt tgatggccgg
     2041 cctggcgttt ctaggcccgt cgatcggcgc ggcgctgggc ggcccactgt cgggccggct
     2101 ggcgacgagg tatccccctg tgctcgtcat ggggctcggt tgtgtggctg ctgccctgtc
     2161 gctggcgacg ctcgcaatat ccagcggccg ggtcgtctat gtaatcgcgc tcagcgcatg
     2221 cggtttcacg atgggcctgg tgtatgcgtt caccaccgtc gcgacgcagg ccgtggtgcg
     2281 acccgagcgc gcaggtgagg cggccggtgt ggcgctgacg tcgttggtga cgctggccgg
     2341 cgtcggtgtc gcggtgtccg gcacgatgct ggaggttttg cggcagggcg gaatgccaac
     2401 ggccagcgga attcccgcaa tcttcctggc catcgcggca gtgctgctgc ccgccgcgat
     2461 cgcggtactc gcctttcgta aacacgaagg aacgctggac caccgctgag ggtcggcggt
     2521 ccagcgtcag acctatcctt cgtggagctc agccggcatc agcccacgct gctggagctg
     2581 ccgttgccgt tattgctgtt ggtgttcgtg ttggaggtgt tatgggacga gttgcccgac
     2641 gccgtgccgc tggcacggtt ggtggcggtg ccacccgtcg cggtaccact ggtggtgctc
     2701 tggttgacgg tagcggcgcc acccgtggtg ttgccgccga ccgtactgcc gctggtggca
     2761 ttgccaccgg cggagttgcc gccggtcgct gcgcctgcga tctgcgaatt cgtaagggtt
     2821 ccgccggttc cgccggtggc gccctggttc acggtgttgg ggtcaacggt tgcggcgcct
     2881 cctgtctggg cctggacgat ggagccgccg gctgcattcc cgccggtgcc ctcaccgccg
     2941 aacccgtcac cgcccgttcc accgccggca agctgaccgg tgaccgcgga gccgccggtc
     3001 gtcggaccgg cctgtccgcc aaaggcggtc gcgccgccga ccgagttgtc gggatcgcct
     3061 gcgagggtcg tgttgatctg ggtcaccgcc tcggtcgtta tgccgccgag cgcagcgccg
     3121 actgcaggac cgccggcgtt cgcggcggcg gtgtttattc cgccggtgcc tgcaccgccg
     3181 agcccgaggc caccggtacc ggcgccgccg agagcactgc ccgcggtgtc cgcggcgtcg
     3241 acgaccgacc cgccgaccgc cgcaccggtg gttcccgcac cgacgttcgt gccgccggtt
     3301 gcggagctgc cggtgttgac ggcgccattc gtgccaccgg cagccacacc accgatggcc
     3361 tctccaccga gtgcgggatt gacgctggac gttgtaccgg ccgcggcgcc ggcggtcgcc
     3421 acaccggcgt tcgtcgcggc actggtccct ataccgccgg tagcggcggc gccgttgact
     3481 gccgtaccgc cgaccaccgg gctgaggacg ttcatctggt tgttcatgat attgccgttg
     3541 aacgcgccgt tttggctgct gttgccgttg ccgttgttcc cgatcgtggt gttgttgccg
     3601 ctgccgatcc accaaccgtc gtcactgctg ttggaaccca aagtgccgac gctgttatcg
     3661 ctatcgtttc cgttgccggt gttggtgttc gagttaacga cgttggttgc caagttgccc
     3721 gccgcgaggc cgattacttc gtttgtggca tcgccacctg ttgcgtcgcc ggccgcggcg
     3781 acgctgttgt tgacggccgc cccacccacg gttgttccac cggttgtatt gccgctggtg
     3841 gcgttaccac cgacgccgta gccgccgttc gcgttgccgg cctgctgacc gttcgcgatc
     3901 gcgccggcct ctccgcccgt ggcgctctgc tgtatcgcat tgccccccac gacggcctcg
     3961 ccgccctcct gatcctgaag gatgcctccg ccgattccgt cgccacccgc cccggcgccg
     4021 ccttcacccg cgccgccagt tcccccgccg ctggcctggc cggtgatcgc gttgccgcct
     4081 gcggtggcac cgaccacacc aggcccggcg accgcgccgc cgatcgagtt atcgggcgag
     4141 gggggagcgt cggggctgag caggccgagg tctcccgttg tattgccctg gactatcacc
     4201 tcactggcgg tgccgccgcc cgctgcgctg gtcgccgctc caccaatgtt tccagcggag
     4261 acattggtac cgccggtcgc cgcgccgccg gcacccccga ggctcgtgcc tgcgccacca
     4321 aggccctgac cggcaatgtc ggggccgtcg agcacctgcc cgcctgtcgc tacgccggat
     4381 gtcgcggtgc cgatattgct gccgccagcg gcaaggccgc cggtgttggc cgcgacattc
     4441 tgacctccga ctgctgcgcc gcctgccccg gtaccgccga ctgccggatt ggcgctgacc
     4501 gtcgtgccga ccgcggcgcc gccgatcgcc ccaccggcat tggtggcggc gctggtccca
     4561 gcgccccctg tggccgcgcc cgcgttgacc gccgtgccgc cgatgatcgg gctcagcccg
     4621 ttgaactggt tgttcatcac gttgccgttg aaggcaccgt tctggctggt gttgccgttg
     4681 ccgttgttgc cgatcggcgt gttgttaccg ctgccgatcc accaaccgtc gttgctgtcg
     4741 ttgtcggcga acgccatcgg cgcgaatgag atcaggccga acccggtagc gcctgcgacg
     4801 gcgcaaatgg cgcacgtgcg attcagcttg gccggtgtgt gggatgcacg cgttttgcga
     4861 tgtttaccca tgctttatct ccttctgact ggtttctgtg tggcgcagag gttttggttt
     4921 agttggaaga tcaacggaca ctcagcaact gctgtgtgca tcacgtcatc cgcatctccc
     4981 ggcggacccc tgcggcttca tgaggcgccc ggctgatctg gcttgatttc cgcctttggc
     5041 ttgagtggct tggtgatccg atcgaccgtg tcgccgaccg catccttgat ccgcgtgacg
     5101 gcgtcgttca gcggacgagg cccgaccacg gtgccagtgc tggaagtcgt accggcctgc
     5161 tggttctgaa gatctagcag ttcccggtta gtgagaccgg gctgtgtgcc actcgtcgca
     5221 gaagtcacct gttcattggt aagcggctct tgcacggcct gcgacgtagt cggcggggtc
     5281 agtgacggta tgaagccgcc aaggccaagg ttcaactggt cgttgtagat atttccggag
     5341 ccggtggcgt tctgagttgt gttgccatta cccgtatcac caacggcgaa gatgttcccc
     5401 agtccgccgg tcgagcccac gttgttccca ctgccgccgc cgtttccact cgcctggttg
     5461 gcattcgcgt agttatagct caggttgctg ccgaggaggc tgaattgcgg gttgatgatg
     5521 ttcgacgtac caacggcagt ctgattcgtg ttgccgtttc cggtcaatcc gaacaggctg
     5581 gttccgaaca gaccgcccga ggtgacactg gtgttgcttc ccgtgccgtt ccctgcggca
     5641 acgttggtga ttgccgtgtt atcgctgacg ttgccgccga acaggctgaa ctgcgggttg
     5701 aaaatattgc cggagaagaa cgcgagctga gtcgtgttgc cgtttccgac cgcgcctccg
     5761 acgaacgtcg acgtcccttc ggacgtcgtg gtggcgccgt tgccattgtt gaccgccaca
     5821 ttgctgatcg tcgtgttatc gctggtgttc ccgcccatca gactcccctg cgggttgaag
     5881 atgttgtagg acaggatgtt gacctgggtg acgttgccgt tgccggtggc gccgccaagc
     5941 gcgatcggcc cgaaaatccc gccgtcggat gtgtcttttc ctccattacc ctgcccgaag
     6001 aagagggcac cgatggtcga gttgtggctc gtgttggaac cgttgccacc gagaccgaac
     6061 tgcgggtcga agacgttgcc ttccaggatg ttgatctggg tggcgttacc ggcgccggcc
     6121 ggaagcagga tggacaacgc ctcggcgtcc ggggcgttga tctcggcgag cagcccaatg
     6181 caggccgccg ctcctcctgc accgatggcg cggacgagca tcggtctgtt cttgcgcggc
     6241 gctcgatgtc gacccagttg ctttttgggc atgggtcgac cagcgccgct tgtatccgga
     6301 gtcatcgctg acccacccct cagataaaag ctgtgtagaa actaagtcgc gcagagggac
     6361 actagaactt tgctgaccgc tgtgtcaacc gtcacagcgg taagagactg gacatacccg
     6421 gcggtatgtt agcgaaggtg caccgggcag cagcgcaaat gatcaggaaa gtgctggtac
     6481 aaagggcaac atgactctat cctggctatt tgcatgtaga gagcacccaa agtccgcaga
     6541 gtgggcagtt gcggttatca gcgctgacgt cagtcggcta ttgtggcagg gactgacatt
     6601 tgtctaatac gagacaatat tattgtaagt gcaattagct cagatcgata tgctagcgtg
     6661 ctgagcccgc tttcggcgcg agtcactcag gtgaatctca gctacagtcg tcccgctccc
     6721 cgcggcagct tcggagtaat ctcgcgacgt ggccacgatg aagatcgcgc gcatccccgc
     6781 ggcgaatgaa aagttcgaac tgaccgaagt cgaaatcccc gagccggacc caggacacgt
     6841 ccgcgtcaag gtccacgcct gcggtgtctg tcattccgat tccctcactg tggccggcgc
     6901 tttgggtaac tccttccctc gcgctcccgg ccacgaaatc gccggcgtgg tcgacgctgt
     6961 cggcgatggc gtcaccacat ggaagcccgg cgatcgtgtc ggcgtcggct ggttcggtgg
     7021 ctgcgatttc acctgcgaac cctgtcgcag aggggatttc atctcgtgcg agaacggccg
     7081 gattcccggc atcgcctacg acggcggcta cgccgagtac atggtcgccc cggccgaagc
     7141 gatcgctcga atccccgacg atctcgccga cgtcgatgcc gccccgctga tgtgtgcggg
     7201 catcaccact ttcaatgccc tacgggagag cgtcgcgcga ccgggcgacg ttgtcgccat
     7261 cctcggggtc ggcggactgg ggcatctcgg catccagttc gcggcaagga tgggcttcga
     7321 agtcgtcgcc atcgcgcgcg gcacggacaa ggagccgctg gcgagagagc ttggcgcgca
     7381 tcactacatc gactccagag cctccgacgt cgccgaggag ttgaacaagc tcggcggcgc
     7441 acaggtggtc ctcgcaaccg tcaccgtcgc cgacgcgatg acagtgacgt tgaacggcct
     7501 caaggcacgc ggccaactcg tcgtcgtcgg cgcgcccaat gacccgctgc aggtgccggg
     7561 ggcggcaatc atcttcaagt cgtcatccgt ccaaggccat gcatcgggaa cgtcgcagga
     7621 ttccgaggac acactgcatt tttcggcact gaccggcgta cggccgatga tcgagaccgt
     7681 accgctgtcg caggcccagg ccgcctacga caagatgatg agcggcgcgg cgcgcttccg
     7741 catggtcctg acgatggagt aggtcccatc gagatcgacg aaatggcgtg atctactcgc
     7801 acttatccgc ccgttcgtcg gtttgggcga gagcaaacga aacacaacga aagacaaagt
     7861 ggcgcccacc ctttcgggtg agcgccactt cggcgttctg gtcaggtcgt caggtgacga
     7921 tcttggtgac ccggccggcg ccaacggtac ggccgccctc gcgaatcgcg aagcgcaggc
     7981 cctcgtccat ggcgacgggc tggatcagct tcaccgagat gtcggtgttg tcaccgggca
     8041 tcaccatttc ggtgccttcc ggcagcgtca ccacaccggt cacatcggtg gtgcggaagt
     8101 agaactgcgg acggtagttg ttgaagaacg gcgtgtgcct gccgccctcg tccttggaca
     8161 ggatgtagac gctgccctcg aactcggtgt gcggcgtggt ggtgccgggc ttgacgacta
     8221 cctggccacg ctccacgtcc tcgcgcttga cgccacgcac cagcagaccg acgttgtcgc
     8281 ccgcctggcc ctggtcgagc agcttgcgga acatctcgac accggtgacc gtggtcttgg
     8341 tggtgccggg cttgatgccg acgatctcga cctcttcgtt gacgttgatc acgccacgct
     8401 ccacacgacc ggtgacgacg gtgccacgac cggtgatcgt gaagacgtcc tcgacgggca
     8461 tcaggaacgg cttgtcggtt tcacgaaccg gatccgggat cgactcgtcg accgcgtcca
     8521 tcagctcctc gacggacttg acccactccg ggtcgccttc gagtgccttg agcgccgaaa
     8581 ccttgatcac cggggcgtcc tcgtcgaatt cctgggcggc cagcagttcg cggacctcca
     8641 gctcgacgag ctcgatgagc tcctcgtcgt cgaccatgtc ggccttgttc agcgcgacca
     8701 ggatgtaggg cacgccgacc tgacgggcga gcagcacgtg ctcacgagtc tggggcatcg
     8761 ggccgtcggt ggcggcgacc accaggatcg cgccgtccat ctgcgctgcg ccggtgatca
     8821 tgttcttgat gtagtcggcg tggccgggag cgtcgacgtg cgcatagtga cgcttctcgg
     8881 tctggtactc cacgtgggag atgttgattg tgatgccgcg cgccttctct tcgggcgcgt
     8941 tgtcgatctg gtcgaaggcc gacgcctcgt tcaactcggg gaacttgtcg tgcagaacct
     9001 tcgtaatagc tgcagtcagc gtggtcttgc cgtggtcaac gtgaccgatg gtcccgatgt
     9061 tgacgtgcgg cttcgtccgc tcgaacttcg ccttcgccac ttctatgtcc tcctggactg
     9121 tgttggtgct tgttgtaaag cagtgtgttg atctttgcag ttgtatggtc ccgccaaagc
     9181 gacgggtccg agattactga cccgtcgcct tcgcgccgtc gattgctcga ctccgggcca
     9241 gctccgagcc tcgggcctgg cggccctcga ttctcactga cccgtcgcct tcgcgatgat
     9301 ctccttcgac acctgcgccg gaacttcagc gtaggagtcg aaaaccatgg agtagttcgc
     9361 ccggccctgg gtcttcgacc gaaggtcgcc gacataaccg aacatctccg acagcggcac
     9421 gtgcgccctc acgacgcgtg cgccactccg ctcctccatg gcctggatct gaccacggcg
     9481 ggagttcagg tcgccgatca cttcgcccat gtaatcctcg ggcgtggtga cctcgacggc
     9541 catgatcggc tccaggatgg ccggttgcgc ttgctgcgca gcctttttca gcacctggga
     9601 accggcgatc ttgaacgcca tctccgacga gtcgacgtcg tggtacgcac cgtcgagcag
     9661 ggtgaccttc aggttcacca acgggtagcc ggccagcacg ccgtactgca tggcgtcctg
     9721 cgcgccggcg tccaccgacg gaatgtactc acgcgggata cgtccaccgg tgaccttgtt
     9781 ctcgaactcg taggtggcac cgtcctcgcc ggtgaacggc tcgatcgaga tgaggacctt
     9841 tgcgaactgg cccgagccac ccgtctgctt cttgtgggtg aactcgacct tgtccacctt
     9901 gcgcttgatg gtctccttgt aggccacctg cggcttgccg acgttggcct cgaccctgaa
     9961 ctcgcgcttc atccgatcga ccaggatgtc caggtgcaat tcgcccatgc cgccgatgac
    10021 cgtctggccc gtttcgacat cttggtgcac cttgaaggtt ggatcctctt cggcgagctt
    10081 ctggatcgac agcgacagct tctcctggtc gctcttggtc ttgggctcga tggccacctc
    10141 gatcaccgga tcggggaagg tcatcgactc cagcacaacc tgctggttcg gatcgctcaa
    10201 ggtgtcgccg gtggtggtgt ccttcagccc gatcaccgca tagatgtgcc cggccgacgc
    10261 gtactcaacc gggttctcct tattggagtg catctggaac agcttgccca gccgctcctt
    10321 cttgcccttg gtggcgttga tgacctgcga accggagtcg accttgcccg agtacacccg
    10381 gacataggtc agcttgccga agaacgggtg cgtggcgacc ttgaacgcca gcgccgcgaa
    10441 cggctcgtcg gtcgacggct cccggatgat gatttcgtcc tctttaccgg gcgcatggcc
    10501 ggtggccggc ggcacgtcca gcggagacgg caggtagtcg atgaccgcgt ccagcatcgg
    10561 ctgcacgccc ttgttcttga acgcgctgcc gcacagcacc aggtaagcct cggaggtgat
    10621 cgtcaacttg cgcagcgcgc ccttgatctc ctcgaccgtg agctcctcgc cgccgaaata
    10681 cttctccagc agcgcctcgt ccgtctcggc gacggcctcg agcagcttgg tgcggtactc
    10741 gtcggcccgc tcctgcagat cggcggggat ctcgatggtg tcgtaggtct cgcccagctt
    10801 ggtctcgccc cgccacacct tggcgttcat ctcgaccagg tcgacgatgc cctcgaattc
    10861 gctctccgag ccgatcggca gctggatcgg gatgacgttc gcgccgaggc ggtcctccat
    10921 ggtcttgacg gagaagtaga agtcagcgcc gaccttgtcc atcttgttga cgaagcagat
    10981 gcgcgggacc tcgtacttgt cggcctgccg ccatacctgc tcggactgcg gctcgacgcc
    11041 ctccttgccg tcgaacacgg caacggcgcc gtcgagcacg cggaggctgc gctccacctc
    11101 cacggtgaag tcgacgtggc cgggggtgtc gatcaggttg atctgatggt ctttccagaa
    11161 cgtggtggtg gcagccgagg tgatggtgat accccgctcc tgctcctgtt ccatccagtc
    11221 catggtggcg gcaccgtcgt gcacctcacc gatcttgtag ctgataccgg tgtagtagag
    11281 gatgcgctcg gtggtcgtcg tcttgccggc atcgatgtgc gccatgatgc c
//
LOCUS       DASYLX010000472         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2106853,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000472 DASYLX010000000
VERSION     DASYLX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106853"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..406)
                     /locus_tag="VGP27_11490"
                     /pseudo
     CDS             complement(97..406)
                     /locus_tag="VGP27_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(873..1149)
                     /locus_tag="VGP27_11495"
                     /pseudo
     CDS             complement(873..1149)
                     /locus_tag="VGP27_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561293.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1510..2067)
                     /locus_tag="VGP27_11500"
     CDS             complement(1510..2067)
                     /locus_tag="VGP27_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015650194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV7851965.1"
                     /translation="MTQELRGQRVGILAADGVERIELEQPRGALHGAGATTEIISLHP
                     GEIQARQWDMESAGSFPVDRVVADVSADDYDALLMPGGTVNPDQLRMNADAVGFVKAF
                     ANTGKPIGVICHGPWTLAEADVIAGVRITSWPSIRTDLRRAGAEVVDEEVVIDRQFVS
                     SRSPNDLPAFCAAIVAQFAKAKQPI"
     gene            complement(2090..>2303)
                     /locus_tag="VGP27_11505"
     CDS             complement(2090..>2303)
                     /locus_tag="VGP27_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851966.1"
                     /translation="GNQDSTTGNQSTDLPAAPGGNPDNRETVQNMGEPELRNAVIELL
                     TIIDDSDDMDVVVTISDDGTVAVAAA"
BASE COUNT          425 a          735 c          731 g          412 t
ORIGIN      
        1 tcgcatggga gctgtggcgg cgaccccaga taggcaccgg cagagcggga accctgcggt
       61 gaggcaagac aatccgctac cggtcagacc acggcgtcag agcttgttgg cgatcacgtc
      121 gagattgctg atgtcgggtg gtgaggcgaa gagctcgtcg gctttctccg cgagggcctt
      181 tccaacgggg ccgccaagat gcgtctgggc gtgccgcctc gtccgggaac gcgtcgatga
      241 tgccgaacgt cgacgggccg aaccgaaccg ctaaccatgg tttcgtgccg ggttcctgct
      301 cgacgagcgg cagcgccgaa agaaaaaatt cctcgactgc gcattctttg ccgggcctgg
      361 cctcgagccg tgccagcaat gcctttgttg aggcccgcga gtccaagact cgtccggcca
      421 cctagctcaa caccgacgga ccagagcaga cccgatatcg acggatggtg gcgcgctcgc
      481 tgagcttcaa gaacccaacg cgctacggcc cacgattcag cagcacaccg acgactgcat
      541 cgacgcgatg ctttccggcc ccgccccccg tcgacctggt ccccgccttg gcgttgcccg
      601 tgccgacgct gacaatttgc gcggtcctcg gggtgccgta cgaacaccac gcggccttcc
      661 aacgcaacgg cgcgatcgga ctcgaccgca actcacccgt cgaggacgcg caacgagcga
      721 tgggcgaaat ctccagtcac gtcagggagt tcgttcagcg cgagatcgac caccccaccg
      781 gcgcagacac catctgcgcc gaaatcgccg agcgggcgcc ggcacgagga tccctaggct
      841 gcgtatgctg ccagcgcgaa agagctaccg taccgggcag tctcgggaac cttcacaagt
      901 gcgacaagcc ccacgaggca ggcagccatc atgtagtagg tgggtaccag cttgtcgccg
      961 gttgcctgga tcagccagtc gttgaccgcc ggagccgtgc caccgaagat cgacgtcgag
     1021 acgttgtagg cggtcgcgaa acccgcatat cgcacctggg tggggaacat cgccggaaag
     1081 gtggcgcaga ccgtcgccaa ccggggacac gtaaagcaaa ctcagtacgg cgaatccgat
     1141 tgcggcgccg ccgtactcgc gaccggtgga gaagccatgg atgccacagg tcggcgctcg
     1201 ctgcttcccc aggaaagatc gactcggcga tatagctcac gccgtaggcg tagatgccgt
     1261 aatcaaacca ctccgtcgcg ttacgaatcg ccgacgcggc gatggcccgt ttcactacgc
     1321 cgggggctgc ttactctcga cagtgggtgg ctttacggat tccatgagcc ggacgcgtag
     1381 tcgaatagcc ggggccacag tggtttccgg agaacactgc ttagacgacg gccacaccga
     1441 cacgcgaacc ggaagtgcga aaccgacact gatccagttc gcgcagccgg agggccgagc
     1501 gtctgcgcgt cagatgggct gtttcgcctt cgcgaactgg gccactattg cggcgcagaa
     1561 agccggtagg tcgttgggtg atcgactgga gacgaactga cgatcgatca cgacctcctc
     1621 atcgacgacc tcggctcctg ctcgccggag gtcagttcgg atgctcggcc atgacgtgat
     1681 cctcactccc gcgatgacgt ccgcttcggc gagggtccat ggaccgtggc agatcactcc
     1741 gatcggtttg ccggtgttcg cgaatgcctt gacgaagccg accgcgtcgg cgttcatccg
     1801 cagttggtca gggttgacgg tgccgcccgg catcagcagc gcgtcgtagt catcggcgga
     1861 cacgtcggcg accacgcggt cgaccgggaa gcttccggcc gactccatgt cccactgccg
     1921 ggcttgaatc tcgccgggat gcagggagat gatctcggtc gtggctcccg caccgtgtaa
     1981 ggctccgcgg ggctgttcca gttcgattcg ctcgacgccg tcggcggcca atatgccaac
     2041 gcgctgaccc cgaagttcct gtgtcatagg atctccagtg ctcgtcggac tacgcagccg
     2101 cgacggcgac agtgccgtcg tctgagatgg tgacgaccac gtccatatca tcggaatcgt
     2161 caatgatcgt taacagctct atcacggcat tgcgtagttc gggttcgccc atgttctgaa
     2221 cggtttcgcg gttgtccggg ttgcctccgg gcgccgccgg taggtctgtg gactggtttc
     2281 cggtggtgct gtcttggttt ccg
//
LOCUS       DASYLX010000473         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2874883,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000473 DASYLX010000000
VERSION     DASYLX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874883"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGP27_11510"
     CDS             <1..204
                     /locus_tag="VGP27_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family protein"
                     /protein_id="HEV7851967.1"
                     /translation="ASLGVPFELPADVVEAVLPLVRAVPDGDFRTMAGAPFAPAVEQV
                     GTDDFEQILRYLGRRPDWRQHME"
     gene            251..1624
                     /locus_tag="VGP27_11515"
     CDS             251..1624
                     /locus_tag="VGP27_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-6-methoxyphenol hydroxylase-like
                     oxidoreductase"
                     /protein_id="HEV7851968.1"
                     /translation="MAKTGEHAIVLGASVAGMLAARVLADYFDTVTVVERDILGDGPD
                     NRRGVPQDRHLHGLLMRGAQVLEELFPGFLDELVAAGSSYFDGNDLSKMYFCMNGHLG
                     VRSGSSRHIKAYSASRPFLEYHVRRRMRVIPNIAVLEGYDFVDMITTENRDRVTGVLI
                     APHGGGEGVSKLLADLVVDATGRAARTPVMLERLGYPRPVEDKVTVHLKYSSQQLRLP
                     PDALHEMGMVVSPIPGRPTGVGLARCEHDTWMLLAFGMAGNDPPDTYAELCDFVEPFA
                     PRHVVAALRSATPLGPTAQHRFPSSRWYRYDRMRRLPDGLIATGDAVCSFNPIYGQGM
                     TVAALDALNLRNCLAEGTTDLPRRFFRASAKPIRQAWQMAAGGDLSLPEIEGTPPLST
                     RLMNPYMDRIMTAAESDVSAFEQFIRVAWLVDSPVNLMRPSIVARAMTAKRRKPAAEP
                     ERVVVQP"
     gene            complement(1615..2001)
                     /locus_tag="VGP27_11520"
     CDS             complement(1615..2001)
                     /locus_tag="VGP27_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851969.1"
                     /translation="MTIEQEPSSDDDASQKARAPADATGQPWWAHHPAIIAAREGVEK
                     WIGSADRESTPPDNSDAIHDEAMNGSCWRELRGAREDLDRARVRYANAVRAARAVGFS
                     WAEIGRLLGVPRQSVHRRFRDDVDYG"
     gene            2106..2927
                     /locus_tag="VGP27_11525"
     CDS             2106..2927
                     /locus_tag="VGP27_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7851970.1"
                     /translation="MAQRDPIHLGSGEPMLLLHPFILSQNVWKRVAPLIADTGQYEVF
                     APTMAGHNGGAKGNFLLDTATLADDVERRLDALGWGTAHIVGNSLGGWVSFELERRGR
                     ARTLTGIAPAGGWTHFTPAKFEIVGKFLAGSPLFLVCQLFGTRAAKFPLSRQLTYPAV
                     SATADALTREDIVEIIDDVAHCPAYVQMLVKSLLLPGLLELAKTRTPTHLVICEKDRV
                     LPTPRYTRLFTEQLPSNTLVTTLDGVGHVPMYEAPQRIADVITGFVDQYAHRKAV"
     gene            complement(2932..4149)
                     /locus_tag="VGP27_11530"
     CDS             complement(2932..4149)
                     /locus_tag="VGP27_11530"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV7851971.1"
                     /translation="MSKIKVVGTVVELDGDEMTRIIWRLIKEQLIMPYLDVHLDYYDL
                     GIEHRDETDDRVTTEAAEAIKRHGVGVKCATITPDEARVKEFHLKRMWLSPNGTIRNI
                     LGGTIFRAPIVISNVPRLVPGWTKPIIIGRHAFGDQYRAFNSKVDRAGTFTVTFTPAD
                     GSEPMVHDVVEIPEDGGVIMGMYNFKKSIQDFARSSFNYALQQNYPVYLSTKNTILKG
                     YDGMFKDEFQTIFDEEYADEFDRRGLTYEHRLIDDMVASCLKWEGGYVWACKNYDGDV
                     QSDTVAQGYGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRQYQQGKPTSTNPIASIF
                     AWTRGLAHRGKLDDTPEVIGFASTLEEVVINTVEGGCMTKDLATLIGPDQPWQTTEEF
                     LGTLADNLERALD"
     gene            complement(4191..>4564)
                     /locus_tag="VGP27_11535"
     CDS             complement(4191..>4564)
                     /locus_tag="VGP27_11535"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV7851972.1"
                     /translation="NKRATLLGTTLDAAIGKLLENNKGPSRRTGELDNRGSQFYLAMY
                     WAQQLAEQTEDQELADHFAGLAKSLAENEDAIVSELAEVQGDSVDIGGYYYPDREKTT
                     AVMRPSKTLNETLESAHARSE"
BASE COUNT          747 a         1504 c         1439 g          874 t
ORIGIN      
        1 gcgtcgctgg gtgtcccctt cgaactaccc gccgacgtcg tcgaggccgt gctgccgttg
       61 gtccgtgcag tgcccgacgg cgacttccga accatggccg gggcgccgtt cgcgccagcc
      121 gtcgaacagg tcggcacgga cgacttcgag cagatactgc gctatctcgg ccgcaggccg
      181 gactggcgtc aacatatgga gtaggcgact acgcacgacc accgctgacg aaggcgtcaa
      241 gatcacattc atggcgaaga cgggtgagca cgcgatcgtt ctcggcgcca gcgtggcggg
      301 catgctcgcc gccagagtgc tggccgacta cttcgacacc gtcacggtgg tcgaacgcga
      361 catcctcggc gacgggccgg acaaccgtcg cggggtgcct caggatcggc acctgcacgg
      421 gttgttgatg cgaggcgcgc aggtgctcga ggaactgttc cccggcttcc tcgacgaact
      481 cgtcgcggca ggctcgtcgt acttcgacgg caacgacctg tccaagatgt acttctgcat
      541 gaacggtcat ctcggcgttc gatcgggcag ttctcggcat atcaaggcct acagcgccag
      601 ccgtccgttt ctcgagtacc acgtgaggcg gcggatgcgg gtaattccca acatcgcggt
      661 gctcgagggg tacgacttcg tcgacatgat caccaccgag aatcgcgacc gcgtcaccgg
      721 cgtcctcatc gcgccgcacg gcggcggcga gggcgtttcg aaactgctag cggatttggt
      781 cgtcgacgcc accggccgcg ccgcccgcac accggtaatg ctcgagcgac tcgggtaccc
      841 gcggccggtc gaggacaagg tcaccgtcca tttgaagtac tcgagccagc agttacggct
      901 gccacccgac gcgctgcacg agatgggcat ggtcgtctcg ccgatccccg gcaggccgac
      961 gggcgtgggc ctcgcccgat gcgaacacga cacctggatg ttgctggcct tcggcatggc
     1021 gggtaatgat ccgccggaca cgtacgccga actgtgcgat ttcgtcgaac cgttcgcgcc
     1081 gcggcacgtg gtggcagcgc ttcgatcggc tactccgctt ggaccgaccg cacagcaccg
     1141 cttcccgtcg agccgctggt atcgatatga ccggatgcgg cgactcccag acggattgat
     1201 cgccaccggc gatgcggtgt gcagcttcaa cccgatctac ggacagggga tgactgtggc
     1261 cgcactcgac gcgctgaacc tgcggaactg cctggcggag ggaacgacag atctgccgcg
     1321 gcgatttttc cgcgcgagcg ccaagcccat ccgccaggca tggcagatgg cggctggcgg
     1381 cgacttgtcg ttgcctgaga tcgagggcac tcccccgctg tcgacgagat tgatgaaccc
     1441 ctacatggat cggatcatga ccgcggcgga atccgacgta tccgctttcg agcagttcat
     1501 ccgagtggca tggctggtgg attcgccggt gaacctgatg cggccgtcga tcgtcgcgcg
     1561 agcgatgacg gccaagcgcc gcaagccggc cgctgagccg gaaagagtag tggttcaacc
     1621 gtagtccacg tcgtcacgga atcggcggtg caccgattgc ctcggcacgc cgagcagccg
     1681 gccgatctcg gcccacgaga aacccaccgc ccgcgcagcc ctgacggcgt ttgcgtaccg
     1741 aacccgagca cgatcgaggt cctcgcgcgc cccgcggagc tctcgccaac atgaaccatt
     1801 catcgcttcg tcgtggatcg catcggagtt atctggcggc gtcgattccc ggtcggcgga
     1861 accgatccat ttctctaccc cctcgcgcgc ggcgatgatc gcagggtgat gggcccacca
     1921 cggctggccg gtggcgtccg ccggagccct tgccttctgc gacgcgtcat catctgatga
     1981 cggttcctgt tcgatcgtca tcatttgatg acggcccgca gaggccacgt agttcccgtc
     2041 tgtgacagat gaggcggatc aagccagctt gcgataccgg cggtaccacc tttactgtcg
     2101 aaatcatggc tcagcgtgac ccgatccacc tcggctccgg cgaaccgatg cttctgctgc
     2161 atccgttcat cctttcccag aacgtgtgga aacgcgttgc tccgttgatc gccgacacgg
     2221 gtcaatacga ggtcttcgct ccgacgatgg ccggccacaa cggcggcgca aagggcaatt
     2281 tcctgctcga tacggccacg cttgccgacg acgtggagcg gcggctcgac gcgctgggct
     2341 ggggaacggc tcacatcgtc ggcaattcgc tcggcggctg ggtgtccttc gaactcgagc
     2401 ggcgtggtcg cgcccgtacg ttgactggta tcgcgcctgc gggcgggtgg acccacttca
     2461 ccccggcgaa gttcgagatc gtcggcaagt tcctcgccgg gtcaccgttg ttcctggtct
     2521 gccagctgtt cgggacgcgc gcggccaagt tcccgctctc acgtcagctc acgtacccgg
     2581 ccgtcagcgc gaccgccgat gccctcacca gagaagacat cgtcgagatc atcgacgacg
     2641 ttgcgcactg cccggcctac gtccagatgc tggtcaagtc gctgctgctg cccggcttgc
     2701 tcgagcttgc caagacccgc accccgaccc acctggtgat ctgcgagaag gacagggtgc
     2761 tgccgacgcc gcggtacacc cgcctgttca ccgagcaact tccgtccaac acgttggtga
     2821 cgacgctgga cggcgtcggt cacgtgccga tgtacgaggc gccgcagcgg atcgccgacg
     2881 tcatcaccgg attcgtcgac cagtacgcgc accggaaagc ggtctgatcc gtcagtcgag
     2941 ggcccgctcg aggttgtcgg ccagcgtgcc gaggaactcc tcggtggtct gccacggttg
     3001 atccggcccg atcagggtcg ccaaatcctt ggtcatgcag ccgccctcga cggtgttgat
     3061 cacgacttcc tcgagagtgc tcgcgaagcc gatcacctcc ggggtgtcgt cgagcttgcc
     3121 gcggtgcgcc aacccgcggg tccacgcgaa gatcgaggct atcgggttgg tcgacgtcgg
     3181 cttgccctgc tggtactgcc ggtagtgccg ggtgacggtg ccgtgcgccg cttccgcctc
     3241 gacggtcttg ccgtcaggtg tcatcagcac cgacgtcatc agtccgagcg atccgtaacc
     3301 ctgtgccacg gtgtctgatt ggacgtcgcc gtcgtagttc ttgcacgccc acacgtagcc
     3361 gccctcccat ttcaggcagg acgccaccat gtcatcgatc aaccgatgct cgtaggtgag
     3421 cccccgcctg tcgaactcgt cggcgtactc ctcgtcgaag atggtttgga actcatcctt
     3481 gaacatcccg tcgtagccct tcaggatggt gttcttcgtc gacaggtaga ccgggtaatt
     3541 ctgttgcagc gcatagttga acgacgaccg ggcgaagtcc tggatcgact tcttgaagtt
     3601 gtacatgccc atgatgactc cgccgtcttc tgggatctcg acgacgtcgt gcaccatcgg
     3661 ctcgctgccg tcggccggag tgaaggtcac cgtgaaggtg cccgccctgt cgaccttgga
     3721 gttgaacgca cggtactggt cgccgaatgc gtggcggccg atgatgatcg gcttcgtcca
     3781 accgggcacc aagcgcggca cgttggagat cacgatcgga gcgcggaaga ttgtgccgcc
     3841 cagaatgttt ctgatggtgc cgttgggcga caaccacatc cgcttgaggt ggaactcctt
     3901 gacgcgggcc tcgtccgggg tgatggtggc gcacttgacg ccgacgccgt gcctcttgat
     3961 ggcctcggca gcctcggtgg tcacccgatc atcggtctcg tcgcggtgct cgatgcccag
     4021 gtcgtagtaa tccaggtgga cgtcgaggta gggcatgatc aactgctcct tgatcagcct
     4081 ccagatgatc cgggtcatct cgtcgccgtc gagttccacg accgtgccga cgaccttgat
     4141 cttggacatg ggccacacta accccaccgg cgtcggctgt gggcgatgcc ctattcgctc
     4201 ctcgcgtgcg cactctccag cgtctcgttg agcgtcttgc tcggccgcat caccgcagtc
     4261 gtcttctcgc ggtccggata gtagtagccg ccgatatcca ccgaatcccc ttgcacctcg
     4321 gcaagttcgg agacgatggc gtcttcgttc tccgccagcg acttggccag tccggcgaag
     4381 tggtcggcca gctcctggtc ctcggtctgc tcggcgagct gctgcgccca gtacatggcg
     4441 aggtagaact ggctgccgcg gttgtcgagc tcaccggtcc ggcgcgacgg gcccttgttg
     4501 ttctccagca gcttgccgat cgcggcgtcg agcgtcgtgc ccaacagcgt ggcccgcttg
     4561 ttgc
//
LOCUS       DASYLX010000474         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1777438,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000474 DASYLX010000000
VERSION     DASYLX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777438"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VGP27_11540"
     CDS             <1..485
                     /locus_tag="VGP27_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7851973.1"
                     /translation="GARVVSVSSGGHKRSPVVFDDIDYAFRDYNPFSAYGQSKTANIL
                     FAVAATTRWAADGITSNALMPGAIFDTRLGRHVDAGHAASAMKQINLKSVEQGAATSV
                     LLAGSPLLDGVGGRYFEDCNEAHVVDRRGEPGTGGVAVYAVDPANAERLWDVSLEMIG
                     "
     gene            488..1252
                     /gene="nei2"
                     /locus_tag="VGP27_11545"
     CDS             488..1252
                     /gene="nei2"
                     /locus_tag="VGP27_11545"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII Nei2"
                     /protein_id="HEV7851974.1"
                     /translation="MPEGDTVFHTATKLREALLGKTLTRCDVRVPRYATVDLTGERVD
                     EVVSRGKHLFIRVGRASIHSHLKMDGSWWVGSIDRPNRAGHRIRIILEAGDVQAAGID
                     LGVLEILERAEDEDAVAHLGPDLLGPDWDPRVAAANLAADPERPLSEALLDQRVMAGV
                     GNVYCNELCFLFGHLPTAPVGSVKDPLRVVQRARDMLWLNRARLMRTTTGDTRRGQQL
                     WVYGRAGEPCRRCGTRIKSDDTGERITFWCPNCQRA"
     gene            complement(1263..2359)
                     /locus_tag="VGP27_11550"
                     /pseudo
     CDS             complement(1263..2359)
                     /locus_tag="VGP27_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
BASE COUNT          465 a          832 c          877 g          443 t
ORIGIN      
        1 gcggtgcccg cgtcgtttcg gtgagttcgg gtgggcacaa gcgctcaccg gtggtattcg
       61 acgacatcga ctatgcgttt cgcgactaca acccgttcag cgcttacgga cagtccaaga
      121 cagccaacat tctctttgcc gttgcggcca cgacacgctg ggcggccgac ggcatcacgt
      181 cgaacgcgct gatgcccggc gcgatcttcg acacccggct ggggcgccac gtcgacgcgg
      241 gtcatgcggc ttcggcgatg aaacagatca acctcaagtc cgttgagcag ggggcggcga
      301 cgtcggtgct gctggcgggg tcgccgttgc tcgacggcgt cgggggtcgg tacttcgagg
      361 actgcaatga ggcgcacgtc gtcgaccggc gaggtgagcc gggcaccggc ggcgtcgccg
      421 tctacgctgt cgacccggcc aatgccgaac ggctctggga cgtctcgctg gaaatgatcg
      481 gatagcgatg ccggaaggtg acaccgtctt ccacacggcg acaaagctgc gcgaggcgct
      541 gctcggtaag acgttgacgc gctgcgatgt ccgggtgccc cgctacgcca ccgtcgacct
      601 gaccggagag cgggtggacg aggtcgtcag ccgaggcaag cacctgttca tccgcgtcgg
      661 ccgggcgagt attcactcgc atttgaagat ggacggtagt tggtgggtcg gcagcattga
      721 ccggccgaat cgcgcgggcc accgcatccg catcatcctg gaggccggtg atgtgcaggc
      781 ggccggcatc gatctcggcg tcctggagat cctcgaacgc gccgaggacg aggacgccgt
      841 cgctcatctc ggacccgacc tgctcggccc ggattgggac cctcgcgtcg ctgcagcgaa
      901 tctggccgca gatcccgagc ggccgctttc ggaggcgctg ctcgaccagc gggtgatggc
      961 aggcgtcggg aacgtctact gcaacgagct gtgcttcctg ttcgggcacc tgcccacggc
     1021 gccggtcggt tcggtgaagg atccgctgcg ggtggtgcag cgcgcccgcg acatgttgtg
     1081 gctgaaccgg gcccgcttga tgcggacgac gaccggggac accaggcgag ggcaacagct
     1141 atgggtgtac ggacgggccg gcgagccgtg ccgccgatgc ggcacacgaa tcaagtcaga
     1201 tgacaccgga gaaaggatca cattctggtg cccgaactgt cagcgggcat gaacgctccg
     1261 cttcagaact tgcggatacc acgcagaatt ctggtgaacg ggatactcgc cgccatccct
     1321 ttgcggaggc tcggctgcag tccactgatg ttgcacacca cgggataccg gaccccctgg
     1381 tcgtgccagt cggcgaattg gtcgatcact tcatcggggg tgccggtcag catgcattct
     1441 ttgagcatcg acagtggaac atcgcgcgta taggacagca cagtctcttc gtcgagggtt
     1501 tgcggaacga agtcctgacc gccgataaag tctgcgccca gcggatgggg taccccatgg
     1561 tgggcccaga tatcagcggg cagattgagc gcgatcgcct tcatcacatc agaagagagt
     1621 gtctcgtcga cctcatcccg gctgcggcca gtgacaacga tgtgccagtt cgcgggaatg
     1681 atcgaggagg ggtcgcggcc ggcgtcggcg gccgctcccc gaatcacctc gagaccggcg
     1741 gcgtagccct tcgcatcgaa acagaacgcc gggaaccaac cgtcggcgta tcgcccggtg
     1801 atccgcagca tcctcggtcg atgtgaggcg atccagactt cgggccacgt ccccttgtgc
     1861 ggcggcagat cgaagatcgc gttgtgcagc gggaagaacg gagagtcacg ggtgacgagc
     1921 tcgccgcccg agttccacag cgcccggatg gtggccatcg cctcttcgaa gcgcgccacc
     1981 ggcgttgacc agtcgacgcc gtactgttca ttgccctccc gctcgccggt cccgatgacg
     2041 agaatcgccc tcccccgcgt catcagatgc agcgtcgcgg ccgcctgggc ggtaacggcg
     2101 gggttccggc ggctcgcatc cgtgacgccg atgccgaggc gcatccgcgc gcgccgagaa
     2161 cggccggcca ggcttccagg acggtccatg gttccagata cgcgtcggcc ttgggggcga
     2221 gccgcgcccc gccgaagtac ttcggcgtca tcaccgagcg cggaaacaat gaattcagat
     2281 ggtcggcgag ccagaacgag tcgacccggc ccgccacgac ggtgccgaaa gccggcctgc
     2341 accgtggccg tcggcgaatg acgcgtgcga accggtaact ctgcgagacc cactttggac
     2401 attgtccgtc ctcccgatgc agcgcctttc aggcacgcca gtggtatctg acgtgcctga
     2461 aggctcaact aagaagacgg ggatacctag agcgatccac taccccaatt cggcggtcag
     2521 cctggcagcg ccagcttgac gatcttgcgc accacggtgg cgaactgctt gggcagcgac
     2581 ccactgttgt agggaatgcc gtaccgcttg cagatct
//
LOCUS       DASYLX010000475         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2106940,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000475 DASYLX010000000
VERSION     DASYLX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106940"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGP27_11555"
     CDS             complement(<1..330)
                     /locus_tag="VGP27_11555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7851975.1"
                     /translation="MSELKIGIILGSTRPGRNGKAVADWVLDKAQARIDADYDLIDLA
                     DHPLPHMDEPMSPMLGQYAREHTKAWAATIAPYDGYVFVTPEYNHSTSGVLKNAIDYL
                     YAEWTNKA"
     gene            422..976
                     /locus_tag="VGP27_11560"
     CDS             422..976
                     /locus_tag="VGP27_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7851976.1"
                     /translation="MARSPRQAAAAATAPKWLTPAQLEAWQALTLMLARLPTALEAQL
                     QRDSQLSYIEYYALAALSERPQRTMRMSELAVLTNAELSRLSHLITRLQKRGYVRREP
                     DPVDGRYTNAVLTDAGYDLVVAAAPGHVAAVRELVIDALDDTALAALQNSAERINARI
                     DDPRHKFSREATASTDSSAESSKQ"
     gene            complement(1007..1756)
                     /locus_tag="VGP27_11565"
     CDS             complement(1007..1756)
                     /locus_tag="VGP27_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7851977.1"
                     /translation="MGNALRNKTAVVTGGGTGIGLAIAKRFVAEGARVFITGRRKEVL
                     DAAVAEIGSGAEAVAADSANLDDLDRLYTTVRERAGSLDIVVANSGSGVLAPLGEITE
                     EQVDQTLGTNVKGVVFTVQKALPLLTDKGSIILIASTVSTRIDVPGQSIYGASKAAVR
                     NLARGWALDLAGSGVRVNAISPGATKTPGLLAGLPGNEEQVMGLMAQGVPLGRIGEPD
                     EIAAAALFLASDEASFVNGIEFFVDGGHAQV"
     gene            2034..2331
                     /locus_tag="VGP27_11570"
                     /pseudo
     CDS             2034..2331
                     /locus_tag="VGP27_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
BASE COUNT          432 a          802 c          747 g          446 t
ORIGIN      
        1 ggccttgttg gtccactcgg cgtagaggta gtcgatcgcg ttcttgagca caccggaagt
       61 ggagtggttg tactcgggcg tgacgaagac atatccgtcg tagggtgcga tcgtcgcggc
      121 ccaggccttg gtgtgctcgc gggcgtactg gcccagcatc ggcgacatcg gctcatccat
      181 gtggggcaac ggatggtcgg ccaggtcgat gaggtcgtag tcggcgtcaa tacgcgcttg
      241 cgccttgtcc aacacccagt cggcgacggc cttgccgttg cggcccggac gggtgcttcc
      301 caggattatc ccgatcttca actcgctcac agcagtcctt tcggcggatg ttcagttgaa
      361 cgttcatgtc acgatagccc gattgacttg aacattcaag tcaatcggtt agaatccttc
      421 aatggcgcga tcccccaggc aggctgccgc ggccgcaacc gccccgaaat ggttgacacc
      481 ggcacaactc gaggcctggc aggccctcac gctgatgctc gcccggctgc ccacggcgct
      541 ggaggcgcag ctgcaacgag attcccagct cagctacatc gagtactacg cacttgccgc
      601 attgtcggag cggccccagc gcacgatgcg gatgagcgag ctcgccgtgc tcaccaacgc
      661 cgagctctcg cggttgtcac acttgatcac gcggctgcaa aagcgtggct atgtgcggcg
      721 cgaacctgat cccgttgacg gccgctacac caatgccgta ctcaccgacg ccggctatga
      781 cctcgtcgtc gcggcagccc ctggccacgt cgcggctgtg cgcgagcttg tcatcgacgc
      841 actcgacgac accgcgctgg cagccctgca gaacagcgcc gaacggatca acgcccgtat
      901 cgacgaccct cgccacaaat tcagccgcga ggccaccgcc tccacggaca gttcagcaga
      961 gagctcgaag cagtgaaaga acttcccggc aagcgctcgg gcggcatcag acctgtgcgt
     1021 ggccgccgtc gacgaagaac tcgatgccgt tgacaaagct cgcctcgtcg ctggccaaaa
     1081 agagggcagc ggcggcgatt tcgtcgggtt cgccgatgcg gcccagcggc acgccctgtg
     1141 ccatcagtcc catcacctgc tcctcattgc cgggcaaccc tgccagcagg ccgggcgtct
     1201 tggtcgcccc gggactgatt gcgttcacgc gcactccact gccggccaga tccagtgccc
     1261 agccgcgggc caggttgcgc accgcggcct tgctggcgcc gtagatgctc tggcccggca
     1321 cgtcgatccg tgtgctcacc gtcgaggcga tcaggatgat cgatccctta tcggtcagca
     1381 gcggcagggc cttctggacg gtgaagacca cgcccttgac attagttccc agcgtttggt
     1441 cgacctgctc ttcggtgatc tcaccgagcg gcgcgagcac tccgctgccg gaattggcca
     1501 ccacgatgtc taggctgccc gcccgctcac gcacggtggt gtagagccgg tcgagatcgt
     1561 cgaggttggc cgagtcggcg gccacagctt ctgcaccgga accgatctcc gcgacggcgg
     1621 cgtcgagaac ctctttgcgt cggccggtga tgaacacgcg cgcaccctcg gcgacgaacc
     1681 gtttggcgat ggccagtccg atgccggtac cgccgccggt gacgacggca gtcttgtttc
     1741 gcagtgcgtt gcccatgcga atctcttctc atattgacgg cggacatacc ggcctcggta
     1801 attccggccg ttttgtatgg cccgctccag caatcgtagg cgttctggaa tgcttggtca
     1861 ataaccgggt accgactggc gtctgtccgg tgaaccgaat cgcgactcgg ttcatgtcat
     1921 cggccccccg aggtctactc cgcgaagaat gttcggactg ccggggacga cgctgcgact
     1981 ccagccagtc ggcacagtgg gtacggagac tacggaaaat ggggagataa accatggcga
     2041 caaaggcatt gctggtacgg ctcgaggcca agcccggcaa agaaggcgca gtcgaggaat
     2101 tccttctttc agcgctgccg ctcgtcgagc aagaacccgg cacgaaacca tggttagcgg
     2161 ttcggttcgg cccgtcgacg ttcggcatca tcgacgcgtt cccggacgag gcggcacgcc
     2221 cagacgcatc ttggcggccc cgttggaaag gcgctcgcgg agaaagccga cgagctcttc
     2281 gcctcgccac ccgacatcag caatctcgac gtgatcgcca acaagctctg acgccgtggt
     2341 ctgaccggta gcggattgtc ttgcctcacc gcagggttcc cgctctgccg gtgcctatct
     2401 ggggtcgccg ccacagctcc catgcga
//
LOCUS       DASYLX010000476         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3424006,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000476 DASYLX010000000
VERSION     DASYLX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424006"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGP27_11575"
     CDS             complement(<1..368)
                     /locus_tag="VGP27_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7851978.1"
                     /translation="MTDETFWCENAWLDGEVAQSVAVGVSAGRITAVEVGALPAASRQ
                     LRGLVIPGMANAHSHAFHRALRSRTQRDRGNFWTWRKLMYQAAGQLTPDTYRTLAAAV
                     YAEMALAGITSVGEFHYLHH"
     gene            complement(365..1600)
                     /locus_tag="VGP27_11580"
     CDS             complement(365..1600)
                     /locus_tag="VGP27_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEV7851979.1"
                     /translation="MDFDTHFDSLWESIIDVGREPATGGYRRFAWNTADLTLREWFTG
                     EAQRRGMTVEEDRNGNTWAWWMPGGWSGDPKGAFVTGSHLDSVPDGGAFDGPLGVVSA
                     FHAVDLVRARGVQPAVPVAVVAFADEEGARFGVACVGSQLSTGALTADRALRLRDNDG
                     NTLADALRGAGRRPEHLGADHTLADRVGVYVELHVEQGRALEDLGSPVAVASSIWPHG
                     RWHLTFGGEANHAGTTRLIDRRDPMLPFASAVHSARIAAGRHNCVATFGKVRVSPNGA
                     NAIPSRVEAWLDARAADEATLATVVDAIRDAAAQHAEVGHVTLEFEAESITPVVAFPD
                     APRRRLQTALSRYGEVPVLPTAAGHDAGILSAKVPAAMMFVRNPTGVSHSPDEYAEIA
                     DCRTGAEALADVMADWVSR"
     gene            complement(1600..>2380)
                     /gene="hutU"
                     /locus_tag="VGP27_11585"
     CDS             complement(1600..>2380)
                     /gene="hutU"
                     /locus_tag="VGP27_11585"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV7851980.1"
                     /translation="RASMARHVKAMVGFMDKGAEVFDYGNSIRDEARKAGYDRAFDFP
                     GFVPAYIRPQFEEGLGPFRWVALSGDPKDIEATDRAILELFGDNAHLRRWITMAEERV
                     AYQGLPARICWLGYGERHRAGLKFNEMVASGELSAPIVIGRDHLDSGSVASPYRETEA
                     MADGSDAIADWPLLNALVNTASGASWVSIHQGGGVGMGRSIHAGQVCVADGTELAAEK
                     LARVLTNDPGMGVIRHADAGYEHAIAVAAQRGVRIPMQENQ"
BASE COUNT          362 a          837 c          803 g          378 t
ORIGIN      
        1 tcgtggtgca gatagtggaa ctcgcccacg gaagtgattc ccgccaaggc catctcggcg
       61 tagacggccg ccgccaaggt gcggtaggtg tccggggtca actgtcccgc ggcctggtac
      121 atcagctttc gccacgtcca gaagttgccg cggtcacgct gcgtgcgcga ccgcagtgcc
      181 cggtggaagg cgtgcgaatg cgcgttggcc atgccgggaa tgaccaggcc gcgcagctgt
      241 cgcgacgcgg cgggcagcgc gccgacctcg accgccgtga tgcgtcccgc gctcacgccg
      301 actgcgacgg actgtgccac ttcgccgtcc agccatgcgt tctcgcacca gaaggtctcg
      361 tcggtcaccg cgacacccaa tcggccatca catccgccag cgcctccgcg ccggtgcggc
      421 agtcggcgat ctcggcatac tcgtcggggg aatgggacac cccggtgggg ttacgcacga
      481 acatcatcgc ggcaggcacc ttggccgaca ggattccggc gtcgtggccc gccgcggtcg
      541 gaaggaccgg aacctcgccg tatcgcgaca gcgccgtctg tagtcgcctg cgcggggcat
      601 cggggaacgc gacgacgggc gtgatcgact cggcctcgaa ctccaaggtg acgtggccga
      661 cctcggcgtg ctgcgccgcc gcatcccgga tcgcgtcgac gacggtggcc aaggtggcct
      721 cgtcggcggc gcgtgcgtcc agccacgctt cgacgcggga cggaatggcg ttggctccgt
      781 tcgggctgac ccggaccttc ccgaaggttg cgacgcaatt gtggcgtccg gccgcgatgc
      841 gcgcggagtg caccgccgag gcgaacggca gcatcgggtc gcggcggtcg atcagtcggg
      901 tggttcccgc gtggttggcc tcaccgccga aggtgaggtg ccatcggccg tgtggccaga
      961 tcgaggaggc gacggccacc ggactgccga ggtcctccag cgcccggccc tgctcgacgt
     1021 gaagctccac gtacacaccg acgcgatcgg cgagtgtgtg gtcggcgccg aggtgttccg
     1081 gtctgcggcc tgcgccgcgg agcgcatcgg cgagcgtgtt gccgtcgttg tcacgcagcc
     1141 gcagcgcccg gtcggccgtc agcgccccgg tgctcagctg cgagccgacg caggccacgc
     1201 cgaaccgggc gccctcctca tcggcgaacg cgacaacggc gaccgggaca gcgggttgga
     1261 cacctcgtgc ccggaccagg tcaacggcgt gaaacgccga caccacaccc agcggtccgt
     1321 cgaacgcacc gccgtcgggc accgagtcga gatgtgatcc tgtgacgaac gcgcccttcg
     1381 gatctccgct ccagccgccg ggcatccacc acgcccatgt gttgccgttg cggtcctcct
     1441 cgaccgtcat gccgcggcgc tgcgcctcgc cggtgaacca ttcgcgcagc gtgagatcgg
     1501 cggtgttcca ggcgaatctg cgatagccac cggtggccgg ttcgcgaccg acgtcgatga
     1561 tggactccca caacgaatca aagtgggtat cgaagtccat tattgattct cttgcatggg
     1621 gattcgaaca ccacgctggg cggctaccgc gatggcgtgc tcgtatccgg cgtccgcgtg
     1681 gcggatgacg cccatgcccg ggtcgttggt gaggacccgg gcgagcttct ccgctgcgag
     1741 ctccgtcccg tcggccacac atacctgacc ggcgtggatc gagcggccca tgccgacgcc
     1801 gccgccctgg tggatcgaca cccacgatgc gccggacgcc gtgttcacca acgcattcag
     1861 cagcggccag tccgctatgg cgtccgagcc gtcggccatc gcctcggtct cccggtacgg
     1921 cgaggccacc gagcccgagt ccaggtggtc gcgtccgatc acgatcggcg ccgacagttc
     1981 gccggaggcg accatctcgt tgaatttcag ccccgccctg tgtcgttcgc cgtagccgag
     2041 ccaacagatg cgggcaggca ggccctggta tgcgactcgt tcttcggcca tggtgatcca
     2101 gcgtctcaga tgcgcgttgt caccgaacag ttccaggatc gcgcggtcgg tcgcctcgat
     2161 gtccttcggg tcaccggaca gcgccaccca ccggaacggg ccgagcccct cctcgaactg
     2221 cgggcgaatg taggcgggca cgaacccggg gaagtcgaac gccctgtcat agccggcctt
     2281 gcgtgcctcg tcgcggatcg agttgccgta gtcgaacacc tccgcgccct tgtccatgaa
     2341 cccgaccatc gccttcacgt gccgggccat cgaggcgcgc
//
LOCUS       DASYLX010000477         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3532289,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000477 DASYLX010000000
VERSION     DASYLX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532289"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..720)
                     /locus_tag="VGP27_11590"
     CDS             complement(34..720)
                     /locus_tag="VGP27_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7851981.1"
                     /translation="MTAVDITSTDDGTVKDRLIRAADAEIVTRGIAAVQMESIARSAG
                     VSRATAFRQLGSVSELLVQVALLRARRHVAEVKTLMAGKVGAFAKLEAAMVYTTRELP
                     TDPSISALIAQHSSSVHDPNVHQVAVDAVGPVLEEGRRNGEVRDDLEIDELVDFLVEQ
                     TYIAAEEPDRSADEVRRRFRHFIIPALAALSGPGGEHVSRTREVEDAVHTAIEALHNL
                     SDQLQRGRPQ"
     gene            complement(731..1960)
                     /locus_tag="VGP27_11595"
     CDS             complement(731..1960)
                     /locus_tag="VGP27_11595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7851982.1"
                     /translation="MTKPLGGIRVLEVAQFTFVPSAGAVLADWGAEVVKVEHPVTGDA
                     QRGLVKVLGRAATAEGSSFAPIMEAPNRGKRSVGLALDNDDARPVLEELIRRSDVFLT
                     NYLPSVRTKLHIDVEDVRRINPDIIYVAGSGFGMNGPDRDAGAYDATAFWARGGSADG
                     VTPPSADTVSFMPAGAYGDNIGGLAIAGGVAAALYGRRVTGEPAVLDVSLLAVGAWTT
                     QFSVNMAMLMGGPLPKFERKTQAPGNPLTGAYRTADDRFVQLSMLQPTRYWPEFCRLM
                     GLADFAEDPRFTSMESMAANAEEAYELVAEAIGKLTYSECKKVLDRGTGQWAPVQDAW
                     ELANDEALIANGRIADIVDAEGNSQKLVASPVKFGDDPVAMSRAPQFAEHTDDVLREL
                     GLSDEELIELKISGAIT"
BASE COUNT          354 a          681 c          645 g          384 t
ORIGIN      
        1 ccataccatc tcgtattgac agttacggca acatcattgc ggccgaccac gctgcagttg
       61 gtcggagaga ttgtgtaatg cctcgatcgc ggtgtggacc gcatcctcga cttcccttgt
      121 ccgggataca tgttcacccc ctggaccgct caatgcggca agggcgggga tgatgaaatg
      181 gcggaaccgg cgtcgcacct catctgctga ccgatcgggc tcctccgccg cgatgtaggt
      241 ctgctccacc aggaagtcga cgagttcgtc gatctccagg tcgtcgcgca cctcgccgtt
      301 tcgccggcct tcctcgagca ccggtcccac ggcgtcgacc gcgacctggt gcacgttggg
      361 gtcgtgaaca gatgaggaat gctgcgcgat gagcgcggat atcgacggat cggtgggaag
      421 ttcgcgcgtg gtgtagacca ttgccgcctc cagcttcgcg aacgctccca cttttcccgc
      481 catcagggtc ttcacctccg ccacgtgccg acgggcccgc agcagcgcca cctgaaccaa
      541 gagctctgag acgctgccca actgacggaa tgcggtggca cgagacactc ccgcgctcct
      601 ggcgatggat tccatctgca cggcggcgat tcctcgggtg acgatctccg cgtccgcggc
      661 ccggatcagc cggtccttga cggtgccgtc gtcggtcgac gtgatgtcga cggccgtcac
      721 gattgaccgc tcatgtgatc gctccggaga tcttgagttc gatgagctcc tcgtcgctca
      781 ggccgagttc gcgtagcacg tcgtcggtgt gttcggcgaa ctgcggagcc cgcgacatcg
      841 cgacgggatc gtctccgaac ttcacggggc tagcgaccag cttctgtgaa ttaccctcgg
      901 catcaacgat atcggcgatt cggccattgg cgatcagtgc ttcgtcgttg gccagctccc
      961 aggcgtcctg gacgggcgcc cactgtccgg tgccgcgatc gagcaccttc ttgcactcgg
     1021 aatacgtcaa cttgccgatc gcctccgcga cgagctcgta ggcttcctcc gcattggcgg
     1081 ccatcgattc catcgatgtg aatctcgggt cctcggcgaa gtcagccagc cccatcagcc
     1141 gacagaactc cggccagtag cgggtcggct gcagcatcga cagttgaacg aaacgatcgt
     1201 cggcggtgcg gtaggccccg gtgagcggat tgccaggggc ctgcgtcttg cgttcgaact
     1261 tcggtaacgg tccgcccatc aacatcgcca tgttgacgct gaactgggtc gtccaggcac
     1321 cgacggccag cagagacacg tcgaggacgg ccggctcacc ggtgacacgc cgaccgtaga
     1381 gcgcagcggc gaccccgccc gcgatcgcca ggccgccgat gttgtctccg taggcccccg
     1441 ccggcatgaa ggacaccgtg tcggcgctcg gcggggtgac gccgtcggcg ctgccgccgc
     1501 gcgcccagaa cgccgtcgcg tcataggcgc cggcgtctcg gtccggtccg ttcattccga
     1561 atccgctgcc tgcgacgtag atgatgtcgg gattgatgcg acggacgtct tcgacgtcga
     1621 tgtgcagctt ggtgcgcacc gacggcagat agttggtcag gaaaacgtcg ctgcggcgga
     1681 ttagttcctc gagcacaggt cgcgcgtcgt cgttgtcgag agcgagaccg acactgcgct
     1741 tgccgcggtt gggcgcctcc atgatcggcg cgaacgagga cccctccgca gtggcggccc
     1801 tgcccagcac cttcaccaga ccgcgctgcg cgtcaccggt caccggatgc tcgaccttca
     1861 ccacctctgc gccccagtcg gcgagcacgg cgcccgcgga cggcacgaac gtgaattgcg
     1921 caacctcgag tactcggatg ccgcctagtg gcttggtcac ggagctccct gcgattacag
     1981 acatcaagtg atgcactttt gcgacgtacg aatcacaaag tatcaggact cgaggcagga
     2041 tgcgagacct ggatcacaaa aggg
//
LOCUS       DASYLX010000478         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1559087,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000478 DASYLX010000000
VERSION     DASYLX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559087"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VGP27_11600"
     CDS             complement(<1..1095)
                     /locus_tag="VGP27_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7851983.1"
                     /translation="MPDTHVVTNQVPPLEDFNPASSPVLTEALIREGGQWGFDEVAEL
                     GALSGSAQAQRWGEIADRNRPVLHTHDRYGHRVDEVEYDPAYHELMKVAVGHGLHAAP
                     WADDRPGAHVVRAAKTSVWTPEPGHICPISMTYAVVPALRYNKELADVYEPLLTSRSY
                     DPELKVPATKAGITAGMSMTEKQGGSDVRAGTTKATPNADGSYSLTGHKWFTSAPMCD
                     VFLVLAQAPGGLSCFFLPRILPDGTRNRMFLQRLKDKLGNHANASSEVEYDGATAWMV
                     GEEGRGVPTIIEMVNLTRLDCTLGSATSMRTGLTRAIHHAQHRKAFGAYLIDQPLMRN
                     VLADLAVEAEAATILAMRMAGATDKAVRGDE"
     gene            complement(1148..1675)
                     /locus_tag="VGP27_11605"
     CDS             complement(1148..1675)
                     /locus_tag="VGP27_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7851984.1"
                     /translation="MSDSETFEQSQQPQPHTKPATTSSGGGAKVVALLSLIVALAAIG
                     VAAWAIVMAWPQKEATPQPTAESKQKVCAAFDTVSKAVQLQTHADLGPDPVAQTAVAA
                     NARLSLIGGGEYLLSRLDDQTPPDLAEAARLFANNLDEIGMNALAGATNDDPQQAARL
                     TAGEDGRNKVANLCK"
     gene            complement(1711..2502)
                     /locus_tag="VGP27_11610"
     CDS             complement(1711..2502)
                     /locus_tag="VGP27_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7851985.1"
                     /translation="MQTTSRGFTIDYTVSGDGPPLLLVPGTLCAARHWVEFGYVSTLR
                     RHWRVIAVDPLGHGYSDTPHHADVYEAAGVTSDLVAVLDATGVAEATVWGYSRGGWLA
                     CNLASRCPERVKRLVVSAYSMSAHEEEVGRILVPLAGFLRDGNWPALWQALGVADPKF
                     QQMMEDTNDAVAVAAAIDGSLRPTRYVDPTSIRCPATYYVGSEDWIVPHVYIDAEKLD
                     ATVDIIDGYGHMGSFFAAAEPVLAAMSARLGVEPLTAWARRRPSA"
     gene            complement(2515..>3472)
                     /locus_tag="VGP27_11615"
     CDS             complement(2515..>3472)
                     /locus_tag="VGP27_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7851986.1"
                     /translation="GFAFNRELVLLYVATGLLAASIGQGRRMLFVIRDWLPFALVLLA
                     YDLSRGAADLIGRPTLWQWPADVDRWLFFGTMPTVWLQERLKLPLPPWWEVVMSTVYM
                     SFFVLPYVVAGVLWLRDREEWKAFVKLFVGLSFAGLVIYALVPAAPPWAAARCTAAEV
                     EGGPPGPPCMFGTARGVPDGGLLGAMQVSQDGANGWVERIVGRGWGKLNMHTASALLD
                     SGQASVNLVAAIPSLHAATTAAIAAFLWNRVHRGWRPFLAAYVLVMAFTLVYTAEHYV
                     IDILLGWALAAVALLAVNRTKSRRAGPSSEMVDVQPGQTVDS"
BASE COUNT          612 a         1141 c         1129 g          590 t
ORIGIN      
        1 ctcgtctccg cggacggcct tgtcggtggc gcccgccatc cgcatcgcga ggatggttgc
       61 ggcttcagcc tcgaccgcga ggtcggcgag aacgttgcgc atcagtggct ggtcgatcag
      121 gtaggcgccg aaagcctttc ggtgctgcgc gtgatggatg gcgcgggtca gaccggtacg
      181 catgctggtc gcgctgccca gtgtgcagtc cagccgggtg aggttgacca tttcgatgat
      241 ggtcggcaca ccacggccct cttcgccgac catccacgcg gtggcgccgt cgtactcgac
      301 ctctgacgac gcgttggcat ggttgccgag cttgtccttg agccgctgca ggaacatccg
      361 gttgcgagtg ccgtctggaa ggatccgcgg caggaagaag caggatagtc cgccgggggc
      421 ctgggccagg acgagaaaga cgtcacacat cggcgcggag gtgaaccatt tgtgtccggt
      481 caggctgtag ctgccgtcgg cgttcggggt cgccttggtg gtgccggcgc gcacgtcgga
      541 gccgccctgc ttctcggtca tcgacattcc tgcggtgata ccggctttgg tggcaggcac
      601 tttcagctcg gggtcgtagg agcggctggt gagcagcggc tcgtagacgt cggcgagctc
      661 cttgttgtag cgcagcgcag gcacgacggc ataggtcatc gatatcgggc agatgtggcc
      721 gggctcgggc gtccacaccg acgtcttggc ggcgcggacg acgtgggcgc ccggccggtc
      781 gtcggcccac ggggcggcgt gcaggccgtg gccgaccgcg accttcatca gctcgtggta
      841 ggcggggtca tactcgacct cgtcgacacg atggccgtag cggtcgtggg tgtgcaggac
      901 cggccggttg cgatcagcga tctcgcccca gcgctgcgcc tgcgcgctgc cggaaagcgc
      961 tccgagttcg gcgacctcat cgaagcccca ttgaccgccc tcgcggatca gggcctcggt
     1021 gaggaccggt gaagacgccg ggttgaagtc ctcgagcgga gggacctggt tggtgacgac
     1081 gtgcgtgtct ggcatgcctc cgatattaca ttttcccgac agccgcacaa gatgcgtaag
     1141 gcgcctgcta cttacagagg ttggcgacct tgttcctgcc gtcctcgccg gcagtcaatc
     1201 tcgctgcctg ctgcggatcg tcgttggtcg ccccggcgag cgcgttcatc ccgatctcgt
     1261 cgaggttgtt cgcgaacagg cgagccgctt cggcgaggtc cggcggcgtc tggtcgtcca
     1321 ggcggctcag cagatattcg ccgccaccga tcagcgaaag gcgggcgttg gccgccaccg
     1381 cggtctgtgc caccgggtcg ggcccgagat cggcatgcgt ctgcaactga accgccttgc
     1441 tcaccgtgtc aaaggccgcg cacacctttt gcttcgactc agcggtcggt tgcggcgtcg
     1501 cctccttctg tggccacgcc atcacgattg cccatgcggc gacaccgatc gccgccagcg
     1561 caacgatgag cgacagcagc gcaacaacct tcgcgcctcc accggacgag gtcgtcgcag
     1621 gcttcgtatg cggttggggt tgctgggatt gctcaaacgt ttcggagtcc gacatgccgg
     1681 aatggtaacg gccaataagc cgctgatccc ttaggcgctc ggccgacgcc gagcccaggc
     1741 ggttagcggt tcaacgccca gtcgcgcact catcgcggcc aggacaggct cggccgccgc
     1801 aaagaaagac cccatatggc cgtaaccatc gatgatgtcg acagtcgcgt cgagcttttc
     1861 ggcatcgatg tagacgtgcg gcacgatcca atcttcggat ccgacgtaat acgttgcagg
     1921 acaacggatc gaggtcgggt ctacatatcg ggtgggtctg agggaaccgt cgatggccgc
     1981 cgctacagcc acggcgtcgt tggtgtcctc catcatctgc tgaaactttg ggtcggcgac
     2041 tccgagggcc tgccagagcg cgggccaatt tccgtctcgc aggaagcccg ccaacggcac
     2101 cagtattcga ccgacttcct cttcatgggc gctcatcgaa taggcgctga cgacgaggcg
     2161 cttcactcgt tcgggacagc ggcttgccaa attgcaggcc aaccaacctc cgcgcgaata
     2221 tccccacacg gtcgcctcgg ccacgcctgt ggcgtccaat accgccacca gatccgatgt
     2281 gactcctgcc gcttcgtata cgtcggcgtg gtgcggggtg tcgctgtatc cgtgccccag
     2341 cggatcaacg gctatgacac gccagtgccg gcgcagggtg gacacatacc cgaactcgac
     2401 ccagtgcctg gctgcacaga gcgttcccgg gaccagcaac aggggcggcc cgtcgcccga
     2461 caccgtgtag tcgatggtga atccgcgtga cgttgtctgc acgcagggat cacatcacga
     2521 atcgacggtc tggcccggtt gaacgtccac catttccgac gacggccccg ctcggcgtga
     2581 cttggtccgg ttgaccgcca acagcgcaac cgccgccagc gcccagccga gaaggatgtc
     2641 gatcacgtag tgctcggcgg tgtacaccag cgtgaaggcc atgacgagca cataagcggc
     2701 caaaaaaggc cgccaccctc gatggacacg attccagaga aatgccgcga tagcggcggt
     2761 cgtcgcggcg tgcaacgagg ggatggcagc caccaggttg acgctcgcct ggcctgagtc
     2821 gagcagcgca ctcgcggtgt gcatgttcag cttgccccaa ccacgcccga cgatccgctc
     2881 gacccagcca ttcgcgccgt cctgactgac ttgcatcgct cccagcaggc caccgtcagg
     2941 aaccccgcgg gccgtgccga acatgcaggg cgggcccggc gggccaccct cgacctctgc
     3001 cgcggtacag cgcgcggcgg cccatggcgg cgccgccggc accaacgcgt aaatcaccag
     3061 acccgcgaag gacagcccga cgaacaactt gacgaacgcc ttccattctt ctcggtcccg
     3121 cagccagagc acacccgcca ccacatacgg aaggacgaag aaggacatgt acacggtgct
     3181 catgaccact tcccaccatg gcggcaaggg cagcttcaac cgctcctgca gccacaccgt
     3241 tggcatggtg ccgaagaaca accagcggtc gacgtcggct ggccactgcc acaaggtcgg
     3301 cctgccgatc agatcggcgg cgcccctgct caagtcgtag gcgagcaaga caagggcgaa
     3361 cggcaaccag tcgcggatga caaaaagcat gcggcggccc tgcccgatgc tcgcggcgag
     3421 caggccggtc gcgacgtaga gcagaaccag ttcgcggttg aacgcgaagc cg
//
LOCUS       DASYLX010000479        11213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3094629,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000479 DASYLX010000000
VERSION     DASYLX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094629"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VGP27_11620"
     CDS             complement(<1..899)
                     /locus_tag="VGP27_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV7851987.1"
                     /translation="MAAVNRVYAARLAGLVVLGPDGESIGRVRDVVISISIVRHQPRV
                     LGLVVELLTRRRIFVPIGRVTAIDPSAVTLVTANVSLRKFSQRPGEVLVLGQVIETRV
                     RVDDPDLPELAGVDVVVVDLGIEQARTRDWLVTRVAVRPLARRLGRRRNVDIVEWERV
                     QGLTPSGLAMPGQGVAQLLEQFQGQRPVEVAEAIRELPEKRRFEVVRALDDERLADVL
                     QELPEDEQANVLRQLEPERAADVLEAMDPDDAADLLGTMTPADAELFLRRMDPEDSED
                     VRRLLAHSPDTAGGLMTSEPVAIA"
     gene            925..1881
                     /locus_tag="VGP27_11625"
     CDS             925..1881
                     /locus_tag="VGP27_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV7851988.1"
                     /translation="MENRYRPRRTILSVPGSSAKMIEKAKSLPADEVFLDLEDAVAPD
                     AKAEARTRVATALADPGWAGQLRGVRVNDWTTPWTYADAIEVVSTAGAALDVIVLPKV
                     TDVSHIHALDLLLSQLETTHGLPVGRIGIEAQIENAQGLTNIDAIAAAPRVQALVLGP
                     ADMAASLNMRTLEVGEQPEGYDVGDAHHHVLMRILIAARTHGVLAIDGPFLKVRDVDA
                     FRRVAGRSAALGYDGKWVLHPDQIAAGNEIFSPRQADYDHAELILEAYEWHTSRAGGA
                     RGAVMLGDEMIDEASRKMALVIAGKGRAAGMAREGERFQPPS"
     gene            complement(1892..2299)
                     /locus_tag="VGP27_11630"
     CDS             complement(1892..2299)
                     /locus_tag="VGP27_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEV7851989.1"
                     /translation="MSSPPEQPDPYAPVDYPTGYSPLPPPVYPQGNAYPVGYPGYPPY
                     DPYRAGKPPGTNGKAIAALVTALAGLVFCGLPSIAGLILGIIAMRECRRTGQDGHGLA
                     LAGTIIGGLVVAMFALYFIFMIGIAASGFQWAP"
     gene            complement(2301..3125)
                     /locus_tag="VGP27_11635"
     CDS             complement(2301..3125)
                     /locus_tag="VGP27_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEV7851990.1"
                     /translation="MTNPGGDSSETPASDSGSGPSEPSSGGYEAPPIEQSQQPDEQSR
                     PQPATEQFPPPAAPTEAPIPPAQPATEHFTPPGGYAAPSTGYQAPPGYQPPPGYQAPP
                     SYQPPTDYQQGGYPPAYPPSPPSQAYPPPPDYQSPGAQQQGYPPPYPDPSAFGGQPNY
                     GAQSYPPPPPPPYGATPGYPPPSYPGAGYPGGYGAGYGQPQPETNQLAVWSLVASLIG
                     ILCTIGSLVGIALGVVALNQIKERRQGGHGLAIAGIAVGVATLIISIIWTVYALSG"
     gene            3253..3780
                     /locus_tag="VGP27_11640"
     CDS             3253..3780
                     /locus_tag="VGP27_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEV7851991.1"
                     /translation="MTSPFQPGQNPGVTPGAPTSPTRGPAALPTPPKGWPIGSYPTYA
                     EAQRAVDHLSDQEFPVQQVTIVGVDLMQVERVTGRLTWPKVLGGGVISGAWLGLFIGL
                     ILGFFSENPWASLITGLVAGVFFGLITSAIPYAMARGTRDFSSTMQLVAGRYDVLCDP
                     QSAEKGRDLLARLTI"
     gene            3997..5394
                     /locus_tag="VGP27_11645"
     CDS             3997..5394
                     /locus_tag="VGP27_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7851992.1"
                     /translation="MRARRLFAAALAAATTASLATSCSSSGGGIVINYYTPASEMATF
                     TAVSKRCNEELGGRFTIQQISLPKEADAQRLQLARRLTGNDKTLDLMALDVVWTAEFA
                     EAGWALPLSDDPSGQAEADATSNTLPGPLETSRWRDKLYAAPITTNTQLLWYRADLVK
                     EPPTTWDDMVAEATRLHDEGKPSWIALQAKQYEGLVVWFNTLLESAGGQVLSGDGKSV
                     TLTDTAEHRAATIKALQIMKAVATAPGADPSITQTDEGTARLALEQGKAVLEVNWPFV
                     LPSMLENAVKGGVGFLPLDKDPALAGSINDAGTFSPSDEQFEAAYDASKKVFGFAPYP
                     GVKAGEQAKVTLGGLNLAVARTTQHRAEAFEAIRCLRNVENQRYTSVEGGLPAVRTSL
                     YDDPAFQAKYPQYAIIRDQLTNAAVRPATPVYQAVSTRISATLAPITDIDPEKTADKL
                     TDAVQKAIDGKGLIP"
     gene            5403..6323
                     /locus_tag="VGP27_11650"
     CDS             5403..6323
                     /locus_tag="VGP27_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7851993.1"
                     /translation="MPARETQAAPAQGAGDDRRSDRKLAYLLIAPAVILMLAVTAYPI
                     GYAVWLSLQRYNLAAPDDTAFVGLSNYQTVLTDRYWWTAFVITLAITIISVAIEFVLG
                     MALALVMHRTIFGKGVVRTAILIPYGIVTVAASYSWYYAWTPGTGYLANLLPEGSAPL
                     TEQIPSLAIVVLAEVWKTTPFMALLLLAGLALVPQDLLNAAQVDGAGAWKRLFKITLP
                     LIKPAILVALLFRTLDAFRIFDNIYVLTGGANNTGSVSILGYDNLFKAFNLGLGSAIS
                     VLIFLSVAIIAFIYITIFGASAPGSDEERR"
     gene            6323..7159
                     /locus_tag="VGP27_11655"
     CDS             6323..7159
                     /locus_tag="VGP27_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7851994.1"
                     /translation="MAERVGAGRATGWIVVNVLVVIYALLPVLWILSLSLKPTSTVKD
                     GKLIPSQITFDNYKGIFSGDAFSSALINSIGIGLITTVIAVIVGGMAAYAVARLAFPG
                     KKLLVGVALLIAMFPQISLVTPIFNLWRSIGLFDTWPGLIIPYITFALPLAIYTLSAF
                     FREIPWDLEKAAKMDGATPAQAFRKVIAPLAAPGIVTAAILVFIFAWNDLLLALSLTA
                     TQRAITAPVAIANFTGSSQFEEPTGSIAAGAMVITIPIIVFVLIFQRRIVAGLTSGAV
                     KG"
     gene            7164..8411
                     /gene="ugpC"
                     /locus_tag="VGP27_11660"
     CDS             7164..8411
                     /gene="ugpC"
                     /locus_tag="VGP27_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV7851995.1"
                     /translation="MAEIVLDRVTKSYPDGATAVQDLSITIADGEFIILVGPSGCGKS
                     TTLNMIAGLEEISSGELRIAGQRVNEKAPKDRDIAMVFQSYALYPHMTVRQNIAFPLT
                     LAKMKKAEIAGKVEETAKILDLTALLDRKPGQLSGGQRQRVAMGRAIVRDPKAFLMDE
                     PLSNLDAKLRVQMRSEIARLQSRLGTTTVYVTHDQTEAMTLGDRVVVMRAGVAQQIGT
                     PEELYARPANLFVAGFIGSPAMNFFPATLTDVGAGLSFGEVTLTQEVHDVLAQHNRPR
                     NVIAGIRPEHIEDASLIDTYARIRALTFEVTVDFVESLGADKYVHFKIEGAGARAAQL
                     AELAAESGVGENEFVARVSTESRVTPGQTVELAFDTTKLVIFDADTGANLTIPPAGAR
                     EEQAAAPSPSAAADVEQSTPAAE"
     gene            8408..8986
                     /locus_tag="VGP27_11665"
     CDS             8408..8986
                     /locus_tag="VGP27_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEV7851996.1"
                     /translation="MTDVLAEVRAHLSAHFAREPDAASVTFLGTQSFDVLRFGPGDDG
                     VIHYVSLGCSREPMTDPADLAADPVRGPRAEVAVSLRGPTPTGLARNVAVLAAAPAVE
                     GLVLAPDALVDLGTPLWSGAPFTAFLLGRSEIADVELAAPSAPVAVLSAIPITATEAA
                     WVRLKGAEAMREAWRTDGVDVLDPGRRASAPS"
     gene            complement(9053..10156)
                     /gene="corA"
                     /locus_tag="VGP27_11670"
     CDS             complement(9053..10156)
                     /gene="corA"
                     /locus_tag="VGP27_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEV7851997.1"
                     /translation="MPSFRGALPPALRPARTKHTHPDARTIHVPVARAMVDCAIYVEG
                     KRLPGKYTHAAALNKVHELENEGKDAFVWIGLHEPDEHQMQAVADVFGLHELAVEDAV
                     HAHQRPKLERYDKTLFLVLKTVNYVEHESMAKAREIVETGEIMVFVGADFVVTVRHGE
                     HSGLAGVRKKLEGSPAILKLGPYAIMHAIADHVVDDYLDVTDLIEVDIDAMEEDIFSP
                     LAHTNIECIYLLKREVVELRRAVNPLTLALQRLHTDHNDLISVEVRRYMRDVLDHNIL
                     ASDRITSYDEMLSSLVQAALGKVAMQQNVDMRKISAWVAIAAVPTALAGIYGMNFEHM
                     PELKWTWGYPAVLTLMFCVCFALYRTFRRNHWL"
     gene            complement(10226..>11213)
                     /locus_tag="VGP27_11675"
     CDS             complement(10226..>11213)
                     /locus_tag="VGP27_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7851998.1"
                     /translation="ETMSPENLAMMRNMLLAMDPPAHTAHRAPLSLHFRARVIGAIEG
                     RVREICRAIMAENHDRGEVDFVHEVASPLPTRVIGELFGLPQQDWAHIQELAERNTSG
                     QDPDIADGGSEAATIEMAMYAMGLAANRRHADRTDDLTTAILEADFGGEPMSDIEFGS
                     FFVQLVTAGNDTTKGMLGSGLLTLLRHPDQLAELRADASLIPAAVEEIIRYDNPLHYF
                     RRTALVDVELNGTHIAAGDKVAMYYTSANRDDDVFSEPQTFDIHRHPNPHLSFGIGSH
                     FCLGVHLARLEGKVFFEELLDSFATIELTGEPVRIRSNLNNTFKRLPVRLSA"
BASE COUNT         1856 a         3644 c         3838 g         1875 t
ORIGIN      
        1 gcgatggcca ccggctcgga ggtcatcaag ccgcctgccg tgtcggggga gtgggccagc
       61 agcctgcgca catcctcgga gtcctcggga tccatccggc gcaggaacag ctcggcatcg
      121 gccggggtca tcgtgcccag caggtcggcg gcgtcgtcgg gatccatcgc ttcgagcacg
      181 tcggcagcgc gttcgggctc cagctgccgc agcacgttgg cctgctcgtc ctccggcagc
      241 tcctggagca cgtcggcgag acgctcgtcg tcgagggccc tgaccacctc gaagcgacgc
      301 ttctccggca gttcacggat cgcctcggcc acctcgaccg ggcgttgccc ctggaattgt
      361 tcgagcagtt gggcgacgcc ctgcccgggc atcgccaggc ccgacggggt caggccctgc
      421 acccgctccc attcgacgat gtcgacgttc ctgcggcggc cgagcctgcg tgccagcggc
      481 cgcaccgcga ccctggtgac cagccagtcg cgggttcggg cctgctcgat gcccaggtcg
      541 accaccacga cgtcgacgcc cgcgagctcg ggcagatcgg ggtcgtcgac gcggacccgc
      601 gtctcgatca cctgaccgag taccagcacc tcgccgggcc gctgcgagaa cttgcgcagc
      661 gagacgttgg cggtgaccag cgtgacggca ctgggatcga tggctgtcac gcggccgatc
      721 ggcacgaaga tgcgccgacg ggtgagcagt tccaccacga gtccgagcac cctgggctgg
      781 tggcggacga tgctgatgct gatcaccacg tcgcggacac ggccgatgga ctcaccgtcg
      841 gggccaagga ccaccaatcc cgcgagtcgg gccgcataga ccctgttcac cgccgccatg
      901 actcaaaggg tagagagtgt gcttgtggaa aaccgctatc gaccccgccg aacgatcctt
      961 tccgttcccg gcagcagcgc caaaatgatc gagaaagcga agtcgctgcc cgccgacgag
     1021 gtgttcctcg atctggaaga cgctgtcgcc ccggacgcca aggcggaggc gcgcacgcgg
     1081 gtcgcgacgg cgttggccga tccgggttgg gcgggacagc tgcgcggggt gcgggtcaac
     1141 gactggacga cgccgtggac ctacgccgac gccatcgagg tggtctccac cgcaggcgcc
     1201 gcactcgacg tcatcgtgtt gcccaaggtc accgacgtgt cccacatcca cgcgctcgat
     1261 ctgctgctga gccagttgga gaccacgcac gggttgccgg tcggccggat cgggatcgag
     1321 gcgcagatcg agaacgcgca ggggctgacc aacatcgatg cgatcgcggc cgccccccgc
     1381 gtgcaggcgc tggtgctcgg cccggcggac atggcggcca gcctcaacat gcgcacgctc
     1441 gaggtcggcg aacaaccgga ggggtacgac gtcggcgacg cgcatcacca cgtgctgatg
     1501 cgaatcctca tcgccgcgcg cacccacggc gtgctcgcga tcgacgggcc ctttctcaag
     1561 gtgcgcgacg tcgacgcgtt ccggcgggtg gccggccggt cggcggcgct gggttacgac
     1621 ggcaagtggg ttctgcatcc cgaccagatc gccgcgggca acgagatttt cagcccccgt
     1681 caggccgact acgaccacgc ggagctgatt ctggaggcct acgaatggca cacctcacgc
     1741 gcaggcgggg cgcgcggtgc ggtgatgctc ggtgacgaga tgatcgacga ggccagccgc
     1801 aagatggcgc tggtgatcgc gggcaagggc cgcgcggcgg gcatggcgcg tgagggcgag
     1861 cggttccagc cgccgagcta ggcgcgaaag gtcatggcgc ccactggaat ccggaggcgg
     1921 cgatgccgat catgaagatg aaatataggg cgaacatggc gaccaccagt ccgccgatga
     1981 tcgtccctgc cagcgcgagc ccgtggccgt cctgcccggt gcgccggcat tcgcgcatcg
     2041 cgatgatgcc cagtatcagg ccggcgatcg acggcagacc gcagaacacc agtccggcga
     2101 gcgcggtcac caacgcggcg atcgccttgc cgttggtgcc gggcggcttg cctgcccggt
     2161 acgggtcgta cggcggataa cccggatacc cgaccgggta cgcgttgccc tgcgggtaga
     2221 ccggaggcgg caacggtgaa tagcccgtcg gatagtcgac cggcgcatag ggatccggct
     2281 gctcgggcgg gctgctcaca tcagccgctg agggcgtaga cagtccagat gatgctgatg
     2341 atcagcgttg cgacacccac cgcgatacct gcgatcgcca gcccgtggcc accttggcgc
     2401 ctttccttga tctggttgag cgcgaccacc ccgagcgcga taccgacgag cgatccgatg
     2461 gtgcacagaa ttccgatcag cgaggccacc agcgaccaga ccgccaactg attggtttcc
     2521 ggctgcggct ggccgtaccc ggcgccgtag ccaccggggt accccgcacc cggatacgac
     2581 ggcggcggat accccggggt cgcgccgtag ggcgggggcg gcggcggtgg ataggactgg
     2641 gcaccgtaat tgggctgccc gccgaacgcc gacgggtcgg gatagggcgg cgggtagccc
     2701 tgctgctgcg cgcccggtga ctgatagtcg ggcggtggcg ggtacgcctg cgaaggtggc
     2761 gaaggcgggt aggcaggcgg ataaccgccc tgctgatagt cggtgggcgg ctgatagctc
     2821 ggcggcgcct ggtaaccggg cggcggctga tacccaggcg gtgcttggta tccggttgac
     2881 ggcgcggcgt agccgcccgg cggggtgaag tgctcggtgg caggctgagc cggcgggatg
     2941 ggcgcttcgg tgggcgcggc aggcgggggg aactgctcgg tggcaggctg cggccgggac
     3001 tgctcgtcgg gctgctgtga ctgctcgatc ggcggcgcct cgtagccgcc ggacgacggc
     3061 tccgacgggc cggaacctga gtcggatgct ggcgtttctg aggagtcccc acccggattt
     3121 gtcatgctgt tcaacctagc gtatgtgctg gtacccgtgg cggagtggac gcggggcacc
     3181 aacgtccgcg ccggatcgtt gaccgtgtgt gaccgaggaa atctcggcgc gaacagagga
     3241 gaacgaggac cgatgaccag tcccttccag ccaggacaaa atcccggcgt gacacctggc
     3301 gcgccgacat ccccgacccg cggaccggcc gccctaccga ccccccctaa gggctggccg
     3361 atcggctcct acccgaccta cgccgaggcg cagcgcgcgg tcgatcacct ctccgaccaa
     3421 gagttcccgg tccagcaggt gacgatcgtc ggcgtcgacc tgatgcaggt cgaacgggtg
     3481 accggacggc tgacctggcc caaggtgctc ggcggcggtg tgatcagcgg tgcctggctg
     3541 ggcctgttca tcggcttgat cctcggcttc ttcagcgaga atccgtgggc gtcgctcatc
     3601 accggcctgg tcgcaggcgt cttcttcggg ctgatcacct cggcgatccc gtacgcgatg
     3661 gcacgcggca caagagattt cagctccacg atgcaactcg tcgcggggcg ttatgacgtg
     3721 ctgtgcgacc cgcagagcgc cgagaagggt cgggatctgc tggcgcgctt gaccatctag
     3781 cgtcggaatt cgcacgctga cgtcgcgcca gcgaggagcg gtgattgcag gtcacgatcc
     3841 tgctgcggct accgacgcgc cgttgcgaag tgttgcaaat cacgcacagc tggctctacg
     3901 gttctggcgc gggagttcct tgcggggccg ctccggcggc gacatcggac gcggagccgc
     3961 ttcggcggcg acatggacgc ggacaggagg cgggtggtgc gcgcacgtcg actgtttgca
     4021 gcggcgttgg ccgccgcgac gacggcgtcc ttggcgacgt cgtgtagttc gagcggcggc
     4081 gggatcgtca tcaactacta cacaccggcc agcgaaatgg cgacgttcac cgccgtttcc
     4141 aagcggtgca atgaggagct gggcggccgc ttcaccattc agcagatcag tttgccgaag
     4201 gaggcagacg cgcagcggtt gcaactggcg cggcgactga ccggtaacga caagaccctc
     4261 gacctgatgg cgctcgacgt ggtgtggacc gccgagttcg ccgaggcggg ttgggcgttg
     4321 ccgctgtccg acgatccgtc cggccaggcc gaggccgacg ccaccagcaa caccttgccc
     4381 ggcccgctgg aaacgtcgcg gtggcgggac aagctctacg cggcgccgat cacgaccaac
     4441 acccaactgc tctggtaccg cgccgatttg gtcaaggagc cgccgacgac ctgggacgac
     4501 atggtggccg aggcgacgcg gctgcacgac gaaggcaagc cgagttggat cgccctgcag
     4561 gccaagcaat acgagggtct ggtggtttgg ttcaacacac tgctggaaag tgctgggggc
     4621 caagtgcttt ccggcgacgg caagtcggtg acgctgaccg acacagccga gcatcgcgcg
     4681 gcaaccatca aggcgctgca gatcatgaag gccgttgcca ccgcacccgg cgcggacccg
     4741 tcgatcaccc agaccgacga gggcaccgcc cgactcgcgc tcgaacaggg caaggccgtc
     4801 ctcgaggtga actggccctt cgtgctgccg tcgatgttgg agaacgccgt caagggcggc
     4861 gtgggtttcc tcccgctgga caaggaccct gcgctggcgg gcagcatcaa cgacgccggc
     4921 acgttctcgc cctccgacga gcagttcgag gccgcctacg acgccagcaa gaaggtgttc
     4981 gggttcgctc cgtacccggg cgtgaaggca ggtgagcagg ccaaggtgac gctgggcggg
     5041 ctcaatctgg cggtggcccg caccacccag caccgcgccg aggcgttcga ggcgatccgg
     5101 tgcctgcgca acgtcgagaa ccagcgctat acgtctgtcg aaggcggact gcccgcggtg
     5161 cgcacgtcgc tgtacgacga tccggcgttc caggccaagt acccgcagta cgcgatcatc
     5221 cgcgatcagc tgaccaacgc cgccgtgcgc ccggccacgc ccgtctacca ggccgtgtcc
     5281 acccggatct cggcgacgct cgcgccgatc accgacatcg atccggagaa gacggccgac
     5341 aagttgaccg atgcggtcca gaaggccatc gacggtaagg ggctgatccc gtgacggcca
     5401 ccgtgcccgc acgagaaacc caagccgcac cggcacaggg cgccggcgac gaccgcagat
     5461 ccgaccgcaa gctggcgtat ctactgatcg cgcccgcggt gatcctgatg ctggccgtca
     5521 cggcgtaccc gatcggctat gcggtgtggc tgagcctgca gcgctacaac ctggccgcac
     5581 ccgacgacac cgcgttcgtc gggctgtcga actaccagac cgtactgacc gaccggtact
     5641 ggtggacggc cttcgtcatc acgctcgcca tcacgatcat ctcggtggcg atcgagttcg
     5701 tgctcgggat ggcgctggcg ctggtcatgc accggaccat cttcggcaag ggcgttgtgc
     5761 gcacagcgat cctgatccct tacgggatag tcaccgtcgc cgcgtcgtac agctggtact
     5821 acgcgtggac gcccggcacc ggctacctgg ccaacctgct gccggagggc agcgcgccgc
     5881 tgaccgaaca gatcccgtcg ctggccatcg ttgtcctcgc cgaggtgtgg aagaccaccc
     5941 cgttcatggc gctgctgttg ctcgcggggc tggcgctggt gccgcaggat ctgctcaacg
     6001 ccgcacaggt cgacggcgca ggcgcatgga agcggctgtt caaaatcact ctgccgctta
     6061 tcaaaccggc gatcctggtg gcgctgctgt tccgcacgct cgacgcgttc cgcatcttcg
     6121 acaacatcta tgtgctcacc ggcggcgcca acaacaccgg ctcggtgtcg atcctcggct
     6181 acgacaacct gttcaaggcc ttcaatctgg gtctggggtc ggcgatcagc gtgctgatct
     6241 tcctgtcggt cgcgatcatc gcgttcatct acatcacgat cttcggcgcg tcggcaccgg
     6301 gatccgacga ggagaggcgc tgatggccga aagggtcggc gcgggccggg cgacggggtg
     6361 gattgtcgtc aatgttctcg tggtgatcta tgcgctgtta ccggtcctgt ggattctctc
     6421 gctgtcgctg aagccgacgt caacggtcaa ggacggcaag ctgattccga gtcagatcac
     6481 cttcgacaac tacaagggca tcttttccgg tgacgcgttc agctcggcct tgatcaactc
     6541 catcggcatc gggctgatca ccacggtgat cgccgtcatc gtcgggggca tggcggccta
     6601 cgcggtggcc cgtctcgcct ttcccggcaa gaaactgttg gtcggtgtcg cgttgctgat
     6661 cgcgatgttc ccgcagatct ccttggtgac accgattttc aacctgtggc gcagcatcgg
     6721 gctgttcgac acctggcccg gcctgatcat cccgtacatc acgttcgcgc tgccgctggc
     6781 catctacacg ctgtcggcgt tcttccgcga gatcccctgg gatctggaga aggcggcgaa
     6841 gatggacggc gccacaccgg cgcaggcctt ccgcaaggtg atcgccccgc tggcggcgcc
     6901 ggggatcgtc accgcagcca tcctggtctt catcttcgcc tggaacgacc tgctgctggc
     6961 gttgtcgctg acggcaaccc agcgggccat caccgcgccg gtggcgatcg cgaacttcac
     7021 cggcagttcg caattcgagg agccgacggg ttcaatcgcg gcaggcgcga tggtgatcac
     7081 gattccgatc atcgtcttcg ttctgatttt tcaacgacgg atcgtcgccg ggttgacgtc
     7141 cggtgccgtg aaaggatagt tcgatggccg agatcgtgtt ggaccgggtg acgaagagtt
     7201 accccgacgg cgccacggcc gtgcaggatc tgtcgatcac catcgccgac ggcgagttca
     7261 tcatcctggt tggtccgtcc ggctgcggaa agtccaccac gctgaacatg attgcggggc
     7321 tggaggagat ctcgtccggc gagctgcgga tcgccggcca gcgggtgaac gagaaggcgc
     7381 ccaaggaccg cgacatcgcg atggtgttcc agtcctacgc gctctacccg cacatgaccg
     7441 tgcgccagaa catcgccttc ccgctgactt tggcgaagat gaagaaggcc gagatcgccg
     7501 gcaaggtgga ggagaccgcg aagattctcg acctgaccgc gctgctggat cgcaagccgg
     7561 gtcaactgtc gggcggtcag cgtcagcggg tggcgatggg ccgcgcgatc gtgcgtgacc
     7621 ccaaggcgtt cctgatggac gagccgctgt ccaacctgga cgcgaagctg cgcgtgcaga
     7681 tgcgctcgga gatcgcgcgt ctgcagagtc ggctgggcac caccacggtg tacgtgacac
     7741 acgaccagac cgaggcgatg acgctcggtg accgtgtcgt cgtgatgcgc gccggggtgg
     7801 ctcagcagat cggcacgccg gaggagctgt acgcgaggcc tgccaacctc ttcgtggcgg
     7861 gcttcatcgg atcgcctgcg atgaacttct ttcctgcgac gctgaccgat gtcggggccg
     7921 ggctgtcgtt cggcgaggtc acgctgaccc aggaagtgca cgacgtgctc gcgcagcaca
     7981 acaggccgag aaacgtcatc gccggcatcc ggcccgagca catcgaggac gcctccctga
     8041 tcgacacgta cgcacgcatc cgcgcgttga cattcgaggt cacggtcgac ttcgtcgaat
     8101 cgctcggtgc ggacaagtac gtccacttca agattgaggg tgcgggtgcc agggcggcac
     8161 agctggcgga gctggcggcc gagtcgggcg tgggcgaaaa cgagttcgtg gcaagggttt
     8221 ccaccgagtc gagggtgaca ccggggcaga cggtggaact ggccttcgac accacgaagc
     8281 tggtcatctt cgacgccgat accggcgcca acctcaccat cccgcccgcg ggcgcgcgcg
     8341 aggaacaggc tgcagcgccg tcaccttcgg ccgcggcgga cgtggagcag tccacacccg
     8401 cagcggagtg accgacgtcc ttgcggaggt ccgtgctcac ctgagcgcgc acttcgcccg
     8461 cgagcccgac gccgcaagcg tgacgtttct gggcacgcag tccttcgatg tgctgcggtt
     8521 cgggcccggt gatgacggcg tcatccacta tgtgtccctt ggctgttcgc gcgagcccat
     8581 gactgatccg gccgacctcg ccgccgatcc ggtgcgtggt ccccgcgcgg aagtggcggt
     8641 ttcgttgcgt gggccgacgc cgacggggct ggcccgcaac gtagccgtgc tggccgccgc
     8701 gcccgccgtc gaaggactgg tgctggcgcc cgacgccctg gtcgatctcg gcacgccgct
     8761 gtggagcggt gccccgttca cggcgttcct gctcggtcgc agcgagatcg ccgacgtcga
     8821 gctcgccgcg cccagcgccc ccgtcgctgt actgtcggca atcccgatca ccgccacgga
     8881 ggcggcgtgg gtgcgactca agggcgccga ggcgatgcgc gaggcgtggc ggaccgacgg
     8941 cgtcgacgtg ctcgacccgg gacgtagggc gtcggcgccg agctgagcac ggctgccggc
     9001 ggacgaccag aggtcccccg accgccggcg ctgtgcccgc agggtcgcgg gcctacaacc
     9061 agtggttgcg ccggaaagtc cggtacagcg cgaagcagac gcagaacatc agtgtgagca
     9121 ccgccgggta gccccacgtc cacttcagct cgggcatgtg ctcgaagttc ataccgtaga
     9181 tgccggccaa cgccgtcggc acggcggcga tcgccaccca cgccgagatc ttgcgcatgt
     9241 cgacgttctg ctgcatcgcg accttgccca gggcggcctg caccagcgag ctgagcatct
     9301 cgtcgtagct ggtgatccgg tcggacgcca ggatgttgtg gtcgagcacg tcacgcatgt
     9361 agcgccggac ctcgaccgag atgaggtcgt tgtggtcggt gtgcagtcgc tgcagcgcca
     9421 gcgtcagcgg gttgacggca cggcgcagct cgaccacctc ccgcttcagc aggtagatgc
     9481 actcgatgtt ggtgtgcgcc agcggtgaga agatgtcttc ctccatggcg tcgatgtcga
     9541 cctcgatcag atcggtgaca tcgagatagt cgtcgaccac gtggtcggcg atcgcgtgca
     9601 tgatcgcgta ggggcccagc ttgaggatcg ccggtgagcc ctcgagtttt ttgcggacac
     9661 cggccaagcc cgagtgctcg ccgtgcctga ccgtcaccac gaaatcggcg ccgacgaaga
     9721 ccatgatctc gcccgtctcg acgatctccc gcgccttggc catcgactcg tgctcgacat
     9781 agttcaccgt cttgagaacc aagaacagcg tcttgtcgta gcgctccagc ttcggccgct
     9841 gatgtgcgtg caccgcgtcc tcgacggcca gttcgtgcag tccgaacacg tcggcgacgg
     9901 cctgcatctg gtgctcgtcg ggttcgtgca ggccaatcca cacgaacgcg tccttaccct
     9961 cgttttccag ctcgtgaacc ttgttcagag ccgccgcgtg ggtgtactta ccgggcagcc
    10021 gcttgccctc gacgtagatg gcgcagtcga ccatggcacg cgcgaccgga acatggatgg
    10081 ttcgggcgtc ggggtgggtg tgcttggtgc gggcggggcg aagggccggc ggcaacgcgc
    10141 cgcggaacga aggcatcggt tagacctcct cttgcagcgc agggctggtc aagcccggct
    10201 aatgctacgc gcgctgcctg ggatttcagg cggataaccg gacgggtagt cgcttgaagg
    10261 tgttgttgag attcgaccgg atccgcaccg gttcgcccgt caactcgatc gtggcgaatg
    10321 agtcgagtag ttcctcgaag aacaccttgc cctcgagccg ggccaggtgt acgcccaggc
    10381 agaagtgact gccgataccg aacgacagat gcgggttcgg gtgtcgatgg atgtcgaaag
    10441 tctgcggctc gctgaacacg tcgtcatcgc ggttggcgga cgtgtagtac atcgccactt
    10501 tgtcgcctgc ggcgatgtga gtgccgttga gttcgacgtc gacgagggcg gtgcgccgga
    10561 aataatgcag cgggttgtcg tagcggatga tctcctcgac ggcggccgga atgagagaag
    10621 cgtctgcccg cagttcggcg agctggtcgg gatgccgcag cagcgtgagc aatccagaac
    10681 cgagcattcc cttcgtcgtg tcattacccg ccgtgacgag ctgcacgaag aaactgccga
    10741 actcgatgtc gctcatcggc tcaccgccga agtcggcttc caggattgcg gtcgtcaagt
    10801 cgtcggtccg atcagcgtga cgccggttcg cggcgagtcc catggcatac atggccatct
    10861 cgatcgttgc ggcttcagat ccgccgtcgg cgatgtccgg gtcctggccg gaagtgttgc
    10921 gctcggccaa ttcctggatg tgcgcccagt cctgttgcgg cagcccgaac aactcgccga
    10981 tcacgcgcgt cggcagcggc gaggcgactt cgtgaacgaa gtcgacttcg ccgcgatcgt
    11041 gattctcggc catgatcgct cggcatatct cgcgcacccg tccctcgatg gcaccgatca
    11101 cccgggcgcg gaagtgcagc gacaggggag cgcggtgcgc ggtgtgggcg ggcgggtcca
    11161 tcgcgaggag catgttgcgc atcatcgcca ggttctccgg cgacatggtc tca
//
LOCUS       DASYLX010000480         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_350915,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000480 DASYLX010000000
VERSION     DASYLX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350915"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGP27_11680"
     CDS             complement(<1..579)
                     /locus_tag="VGP27_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV7851999.1"
                     /translation="MSTTHTVDGRPRARALGIAPPGEPGPLNAITDVGGVEVGVTTLI
                     DGEGPLIVGTGPVRTGVTAILPRGRAGVGEPCAAGWYSLNGNGEMTGTTWIEEAGSFD
                     LPVVLSNTHAVGACHTGVIRWVNRVDPRLARQWLLPVCAETWDGYLNDINGGHVRPEH
                     VEAALDAATTGAVGEGSVGGGTGMNCYGYKGGN"
     gene            641..2044
                     /locus_tag="VGP27_11685"
     CDS             641..2044
                     /locus_tag="VGP27_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEV7852000.1"
                     /translation="MTQGGRSGSALTAPSFSGRTPSGTGDLSVETHGIAPIREDQRYG
                     STRRLFSVWFAPQVNMTGVFTGALAIALGLGFWLGMLAMVIGTVLGSLVVGYLSTWGP
                     RTGTAQLPNSRMAFGGGVILPGVLQWLSSIAWDALVGLFGGEALAVLLGIPFWSAVLI
                     VLAVQGAVGFFGYEMIHRIQAVLTVVLFVTFVVFAVKLAAGHEVVAPPAVHGADLVGA
                     FILEVTIALSLSISWASYAADFSRYLPADSSRLRVFGFTFAGIVLAYTFVQGIGIAAG
                     DLVSEHTAEGVRTVMGGGIIGALALLIIALASIGSGVMNDYSGSLALQTIGVRVRRPI
                     SAVVVTVIAFALILWLHGGDTASRFENMLLLVSYWIPAFVAVVVIDWALRVRGRPTIN
                     PAEEYTGRGDAIAAVVAFVLAYAAAVPFMNISLFTGPVARAWHGADIAYFVNFLVAAL
                     FYGGYRLLRSRPANFDR"
     gene            complement(2002..2526)
                     /locus_tag="VGP27_11690"
     CDS             complement(2002..2526)
                     /locus_tag="VGP27_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7852001.1"
                     /translation="MATAWRLRNGNADDLDFVGPLWVAVHHRHCESMPELAPYVDDAE
                     TWSARRTLYEGLLAKPETLLLIALVDEQPIGYGLTHVMPVADSWIEDTWRTGDRIGEI
                     ESLSVLPEYRGSGLGSELLERLEAHLHDLGVEDVILGALAGNDHAIRLYERRGYRPTW
                     LYLSKFAGRERNSR"
     gene            2728..3249
                     /locus_tag="VGP27_11695"
     CDS             2728..3249
                     /locus_tag="VGP27_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEV7852002.1"
                     /translation="MAQQSESEGDEERQRLADRVLGRLEDAVYWGIALVLIIGSVALL
                     IAQFNTMLTLRTGPAKTVMLEILDGLLLIFILVELLYAVRACLRSHEIVAEPFLIVGI
                     LAGIKEIVVLSVEAATLLDKGPAFARAVVEIGVLAGVVLALALAAFVLRERRRDTQDV
                     GEDCADEEQPSKT"
     gene            3387..>3626
                     /locus_tag="VGP27_11700"
     CDS             3387..>3626
                     /locus_tag="VGP27_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017205476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7852003.1"
                     /translation="MVVSAALLIRERGAQATAISDVLKHSGAPRGSAYHYFPGGRMQL
                     LEEATDYAGAYIADKISAAESAVELLDDLTELYRRQ"
BASE COUNT          538 a         1179 c         1225 g          684 t
ORIGIN      
        1 gttgccgccc ttgtacccat agcagttcat gcccgtgccg ccgccgaccg agccctcgcc
       61 gactgcgccc gtggtggcgg cgtccagtgc cgcttcgacg tgctccgggc ggacgtggcc
      121 gccgttgatg tcgttgagat agccgtccca cgtctcggca cacacgggca gcagccactg
      181 gcgcgcgagc cgcgggtcga cccggttgac ccaccgaatg acgccggtgt ggcaggcgcc
      241 caccgcatgc gtattcgaca acacgaccgg cagatcgaac gacccggcct cctcgatcca
      301 ggtggtgccg gtcatctcgc cattgccgtt gagcgaatac cagccggccg cacacggttc
      361 gccgaccccg gcgcggccgc gcggcaatat cgcggtgaca ccggtgcgga cgggacccgt
      421 gccgacgatg agcgggccct cgccgtcgat caacgtcgtc acacctacct cgactccgcc
      481 gacgtcggtg atggcgttga gcggtcccgg ctcaccgggc ggcgcgatgc cgagcgcccg
      541 ggcccgcggc cggccgtcga cggtgtgtgt tgtgctcacg tcagggaatc taggcggaca
      601 cggcgtctgc cgcgttatct cgtcatggtc gaatggaccc atgacacaag gcggcagatc
      661 cggaagcgcg ctcaccgcgc cgagtttcag cggccgtacg ccgtccggca cgggggacct
      721 gtcggtcgag acgcacggca tcgcgccgat cagggaggac cagcgctacg gcagcacgcg
      781 aaggctgttc agcgtgtggt tcgcaccgca ggtcaacatg accggtgtct tcaccggtgc
      841 actggcgatc gcgctggggc tcgggttctg gctcggcatg ctcgccatgg tgatcgggac
      901 ggtgctgggc tcactggtgg tgggctacct gtcgacgtgg ggtccgcgca cgggcacggc
      961 ccagctgccg aactctcgga tggctttcgg cggcggggtg attcttcccg gtgtgctgca
     1021 atggctttcg tcgattgcgt gggatgcgct ggtcgggttg ttcggtgggg aagcacttgc
     1081 cgtgttgctc ggcatcccgt tctggagcgc ggtcctcatc gtgctcgccg tgcagggcgc
     1141 ggtcgggttc ttcggctacg agatgatcca tcgaatccag gcagtgctga cggtggttct
     1201 gttcgtcacc ttcgtcgtgt tcgccgtcaa gctcgcggca ggccacgagg tcgtcgcgcc
     1261 gcccgcagtg catggggccg acttggttgg agcatttatc ctcgaggtca ccatcgcact
     1321 cagcctgtcc atctcgtggg ccagctacgc ggccgacttc agccgttatc tgcccgccga
     1381 ctcgtcccgc cttcgtgtct tcgggttcac gttcgccgga atcgttttgg cgtacacctt
     1441 cgttcagggc atcggcatcg cggcgggcga cctggtgtcc gagcacaccg ccgagggcgt
     1501 ccggacggtg atgggcggcg gcatcatcgg ggcactggca ctgctcatca tcgcgctggc
     1561 gtcgatcggc tcgggcgtga tgaacgacta cagcggatcg ctggcattgc agaccatcgg
     1621 ggtgcgcgtg cgcaggccga tctcggcggt cgtcgtcacc gtgatcgcgt tcgcgctgat
     1681 cctctggctg cacggcggtg acacggcgtc ccggttcgag aacatgctgc ttctggtcag
     1741 ctactggatc ccggcgttcg tcgccgtggt cgtcatcgac tgggcgctgc gcgtgcgagg
     1801 gcgtccgacg atcaatcccg ccgaggagta caccggccgc ggcgacgcga tcgctgcggt
     1861 ggtcgcgttc gtgctcgcct acgccgccgc ggtgccgttc atgaacatct cgctgttcac
     1921 cggtcccgtc gccagggcgt ggcacggagc cgacatcgcc tacttcgtca atttccttgt
     1981 cgcagcgctt ttttacgggg gctaccggct gttgcgctcg cgtcccgcga acttcgacag
     2041 gtagagccag gtgggtcggt agccgcgtcg ttcgtacagc cggatggcgt ggtcgttgcc
     2101 ggccagcgcg ccgaggatga cgtcctccac gccgaggtcg tgcagatgtg cctcgagacg
     2161 ctcgagcagt tctgagccga ggccacttcc gcggtattcg ggcagcacgc tgagcgactc
     2221 gatctcgccg atccggtcgc cggtccgcca ggtgtcctcg atccacgaat ccgccaccgg
     2281 catcacgtgg gtcaagccgt agccgatcgg ttgctcgtcg accagcgcga tcagcagaag
     2341 ggtctccggt ttcgccagca gcccttcgta gagcgttctg cgggcagacc aggtttcggc
     2401 gtcgtccaca tacggagcaa gctcgggcat ggactcgcag tgccggtgat ggacagcgac
     2461 ccacagtggt ccgacgaaat cgagatcgtc tgcgttgccg ttccgcagcc gccacgcagt
     2521 cgccatccgg tcacgctagc tcatccggcc cggagaggat ctcggtcatc gcggtgttta
     2581 attaatgtcc atgcgcccgg gaaccgtcgc gacatcggca gccacggtct tcgtctcggc
     2641 tgtggtccga ggcctggcga gtcggcccgc cgactccggc tggtacgcca ggccgcggaa
     2701 accgtcgttc cacttgaacc gctgaccatg gcgcagcagt cggaatccga gggagacgag
     2761 gagcgccagc gcctcgccga ccgagttctg ggcaggctgg aagacgcggt ctattggggg
     2821 atcgcgctgg ttctgatcat cggctcggtc gcgctgttga tcgcacagtt caacaccatg
     2881 ttgacgctgc gcactgggcc cgcgaaaacg gtgatgctcg agatcctgga cggcctactg
     2941 ctcatcttca ttctcgtcga gctgctctac gccgtgcggg cctgcctgcg atcacacgag
     3001 atcgtggcag aaccgttcct gatcgtcggc atcctggccg gcatcaagga gatcgtcgtg
     3061 ctgtccgtcg aggccgcgac gctgctggac aaggggcccg cctttgcccg cgcggtcgtc
     3121 gagatcggcg tgctcgccgg cgtcgttctg gcgctggcgc tggccgcctt cgtgctgcgt
     3181 gaacgtcgtc gggacacaca ggacgtcggc gaggattgcg cagacgaaga gcagccctcg
     3241 aaaacctgac ggcccggctg cggagcgctc gctgtggtac agattcgcag gtcgaaaccg
     3301 tgcgtgagtg atcgttcgcg gacgctagcc agccgtccgt tatgtatgcc atcatatata
     3361 gtgtggtcag caacccgcgg gaccggatgg tcgtatcggc cgcactgttg attcgcgaac
     3421 gcggtgccca ggcaaccgcg atctctgatg tgctgaagca cagcggtgcg ccgcggggat
     3481 cggcgtatca ctacttcccc ggcggcagga tgcaactgct cgaagaggcg accgactacg
     3541 ccggcgccta catcgcggac aagatctcgg ctgccgaaag cgccgtcgaa ctgttggacg
     3601 acctgacgga gttgtaccgc aggcaa
//
LOCUS       DASYLX010000481         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_242302,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000481 DASYLX010000000
VERSION     DASYLX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242302"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..110
                     /locus_tag="VGP27_11705"
     tRNA            28..110
                     /locus_tag="VGP27_11705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61..63,aa:Leu,seq:cag)
     gene            372..896
                     /locus_tag="VGP27_11710"
     CDS             372..896
                     /locus_tag="VGP27_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852004.1"
                     /translation="MSGATRTVAFLLFTLVLMIGSAASPSPSNASPDGGVVGGYALLP
                     PRILHWPPAPVADDMHLSNSLRETSGPASPLETINMVRLFGTAAVSAQERAVATAACE
                     AMNLAFLGAGQRDFLKEAIYSNVNEAEFNAGPFAEAGINSAIERAARAISAARYGDGL
                     LKFYLATCYRPKHG"
     gene            complement(972..1826)
                     /locus_tag="VGP27_11715"
     CDS             complement(972..1826)
                     /locus_tag="VGP27_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852005.1"
                     /translation="MLRDIRELTNDPEAYAAELRSRWGGLLSYRYIGRFHASMDNGTA
                     DDTVTVRRDMRNPSGGLLLAVLGIASPDSGGVSDLEAVPNPVIWSGQILDPGEDIERF
                     EVKTEVLKQGRQMAYSRSTIVDADNPDRVLALTEGQGVSIGVPPDGLQKMEHVPLEIV
                     DSPDLPPLWQAFGGACRPDGHWVLPKLSAEVASPDAALHVGPQFVILETAAFDVACGA
                     AGTNRLQGTSSHVMFLARGKIGPFRVEAQPIVGADGTVAARAVLYDEGNDDKAITAAS
                     YVFRYDTG"
     gene            complement(1850..2434)
                     /locus_tag="VGP27_11720"
     CDS             complement(1850..2434)
                     /locus_tag="VGP27_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852006.1"
                     /translation="MGTTRHRRQGSRRDPAIDEAVLAATRTLLVERGYSATTIDLIAV
                     TAGVSRPAVYRRWTSKARLVHEAVFPDLGPEPPADDFAGEIARLCTGAVAMYRDPAVR
                     EAIPGLMVDLRADRRMRRVISDRLEAAARSQLAAKVHAAADAGVARRGVSADTLMDMI
                     AGGAWYAVCVRHVTNTDRAAKELTDLVLRGVLSG"
     gene            2561..>3466
                     /locus_tag="VGP27_11725"
     CDS             2561..>3466
                     /locus_tag="VGP27_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7852007.1"
                     /translation="MTAGLTGPVAATGEDGRHLYTCPLCEAMCGLEIQVEGGRVASIR
                     GNRDDTWSRGHICPKGATLGALHEDPDRVRSPMIKVDGQWREVSWDAAFRRCTELLAP
                     VIENHGIGAVTCYTGNPLAHSFSLGRYTGVLLGMSGIPLSYSPGTVDQWPKNLSSHLM
                     YGGWWTFPVPDVERTDLLVVMGANPAASQGSLLAAPDVMGIIDGIRKRGRVIVIDPVR
                     TATAARADEWLPITPGTDAALLLAVAHTLFAEDLVNLGGLAPHIDGLDRMREVAAQWP
                     PERVAAVTKIDAERIRELARELAGTR"
BASE COUNT          575 a         1169 c         1141 g          581 t
ORIGIN      
        1 cgcagtgata ggctgccccg gttgttgggg cgagtggcgg aatggcagac gcgctggctt
       61 caggtgccag tgtccttcgg gacgtggggg ttcaagtccc ccttcgccca caattgtgat
      121 gagtcgagtc atgggttaca cctgagtcgc cccatcggtt acagatcccc cggccatcgg
      181 ccggggtttt tctgtttggc gcgccgatag aggcggcgcg ggggtttcta cgcgcgaccc
      241 atccgttctg agcgggccta ccaccagcga attgacgtag cgaactactc gtgtcaaggc
      301 tgagccctcc gaccaaactt gcaattcatc aggagcaaaa ccctgcacgc atcggttcgg
      361 cggaggtcat catgagcggc gcaacacgta ccgtggcatt ccttctcttc acgctggtgc
      421 tgatgatcgg ctcggcggcc tcgccctcac cttcgaacgc gtcacccgat ggcggcgtcg
      481 tagggggata cgctctcctc ccacctagaa ttctgcattg gccaccagct ccggtcgccg
      541 acgacatgca tctgtcgaat tcactgcggg agacctccgg ccctgcatcc ccgctcgaaa
      601 ccatcaacat ggtccggctt ttcgggacag ccgcggtcag tgctcaagag cgggcggtgg
      661 cgacggccgc ctgtgaggca atgaatctcg ccttcctcgg tgccgggcaa cgcgacttct
      721 tgaaggaagc aatctactcg aacgtcaatg aggcggagtt caacgccggg ccgttcgcgg
      781 aggcgggaat caactccgcc atcgagcggg cagcgagggc catctccgcc gcccgatacg
      841 gcgacggact tctcaagttc tacctcgcga cgtgttaccg gccgaagcac ggctgacgag
      901 gcggccatgg cggcactcca ccgtcaatgc gcagcccggc gggacggccg tagctcacct
      961 ttgccgccga attaccctgt gtcataacgg aaaacgtacg acgcggcggt gatcgccttg
     1021 tcgtcgttgc cttcgtcata caggacggca cgcgcggcga cggtgccgtc ggcgccgacg
     1081 atcggctgcg cctcgacacg gaagggcccg atcttgccgc gggcaagaaa catcacgtgc
     1141 gacgacgtgc cctgaagtcg gttggtgcct gccgcgccgc acgctacgtc gaaggcggct
     1201 gtctcgagga tgacgaactg ggggccgacg tgcagcgcgg cgtcgggtga tgccacctcg
     1261 gcggacagct tcggcagcac ccagtgcccg tcgggccggc acgctccgcc gaacgcctgc
     1321 cacaacggcg gtaggtcggg ggagtcgacg atctcgagcg ggacgtgctc catcttctga
     1381 aggccgtccg gcggaacgcc gatgctgacg ccctggccct cggtcagcgc gagcacccgg
     1441 tcggggttgt cggcgtcgac gatggtcgag cgactgtagg ccatctgccg tccctgcttg
     1501 agcacctcgg tcttgacctc gaagcgctcg atgtcttcgc ccgggtcgag gatctggccg
     1561 gaccagatga cgggattcgg cacggcctcg aggtcggaga cgcctccgct gtcgggtgac
     1621 gcgataccga gcaccgcgag caaaagtccg ccggacggat tgcgcatgtc gcggcgcacc
     1681 gtgacggtgt cgtcggcagt gccgttgtcc atcgaggcgt ggaagcggcc gatgtagcga
     1741 tagctcagca gcccgcccca gcggctgcgg agttccgccg catacgcctc ggggtcgttc
     1801 gtcagctccc ggatgtcacg aagaattttc ggtgctcctc acgtgtgcgt tacccgctca
     1861 gcacaccgcg cagaacgaga tcggtcaact ccttggccgc ccggtcggtg ttggtgacgt
     1921 gtcgcacgca caccgcgtac caggcgccgc ccgcgatcat gtccatcaac gtgtcggcgc
     1981 tgacaccgcg tcgggccacg cccgcatctg ccgcggcatg gaccttggcg gccagttggc
     2041 tgcgggcggc ggcctccagc cggtcgctga ttactcgacg catccgccgg tcagcgcgca
     2101 gatcaaccat caaacccgga atcgcttcgc gcacagcggg atcgcggtac atggccacgg
     2161 ctcccgtgca tagccgggcg atctcgccgg cgaagtcgtc cgccggcggt tcgggaccga
     2221 ggtcggggaa gacggcctcg tgcacgagcc gcgccttcga tgtccaacgg cggtataccg
     2281 ccggcctgct caccccggcg gtgacggcga tcaggtcgat ggtcgtcgcc gagtagccgc
     2341 gctcgaccag cagcgtccgc gtcgcggcca aaacggcctc atcgatggcc gggtcgcggc
     2401 gcgagccctg ccgacgatgc cgtgtcgtgc ccacgatgcc cttcccggga tgcggatttg
     2461 tcgatacagt ctgtcacatc aaccgcgaaa tagcttcctg gctgtgcttc cgccgaaatt
     2521 ccgacccgga atgctatttc gcgcgaagag gggagcggag atgaccgcgg gactgaccgg
     2581 gccggtggca gcgaccggag aagacggcag gcatctctac acctgcccat tgtgtgaagc
     2641 catgtgcggc ttggagattc aggtcgaggg cggcagggtc gcgagtatcc gcggcaaccg
     2701 ggacgacacc tggagccgag ggcacatctg ccccaagggc gcgacactgg gcgccctgca
     2761 cgaggacccc gaccgcgtcc ggagcccgat gatcaaggtc gacggccagt ggcgcgaggt
     2821 cagctgggac gccgccttcc ggcgctgcac cgaactgctc gccccggtca tcgagaacca
     2881 cggcatcggt gcggtgacct gctacaccgg taacccgctc gcgcattcct tctccctcgg
     2941 ccgctacacc ggcgtgctgc tcggcatgtc cggaatcccg ctgagctatt cacccggcac
     3001 cgtcgaccag tggcccaaga acctttcgtc gcacctgatg tacggcggtt ggtggacctt
     3061 tccggtgccc gacgtcgaac gcacggacct gctcgtcgtg atgggcgcca acccggccgc
     3121 atcgcaggga tctctgctcg ccgcacccga cgtaatgggc atcatcgacg gaatccgcaa
     3181 gcgcggcagg gtcattgtca tcgatcccgt tcgcaccgcg accgctgcgc gcgccgacga
     3241 atggctgccg atcacgccgg gcaccgacgc ggcgctgctg ctggccgtcg cccacacgct
     3301 gttcgcggaa gatctcgtca accttggtgg gctggcgccg cacatcgacg ggctggaccg
     3361 gatgcgggag gtggccgcgc agtggccacc cgaacgggtc gcggcggtca ccaagatcga
     3421 cgccgaacgc atccgcgaac tggcccggga actcgcaggc acccga
//
LOCUS       DASYLX010000482         5206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2436092,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000482 DASYLX010000000
VERSION     DASYLX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436092"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VGP27_11730"
     CDS             <1..140
                     /locus_tag="VGP27_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7852008.1"
                     /translation="RLGWHWAKFAFGSWMTLSWVDHNVWRRVVPKGWFYNVMVTGVKP
                     S"
     gene            74..1300
                     /locus_tag="VGP27_11735"
     CDS             74..1300
                     /locus_tag="VGP27_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852009.1"
                     /translation="MASRRPEGLVLQRHGDRGQAVVDFGLDAVAYLNSDAGAQALRDV
                     DGYRLTDASRISDIGSIRVQFGEHAPALVETVLLRRRARAKFADPSDWLFTDDALQQA
                     TAQPVAEHRARRLAGATVHDVTCSVGTELAALRKSAGYVMGSDIDPVRLAMARHNLGD
                     VPLCRADALRPVTRDAVVLADPARRRSGRRRFGLLDYTPPLDRLLDVYRHRDVVVKCA
                     PGIDFAQLERLGFNGEVEITSLGGSVREACLWSPGLAATGVRRRATMLDGAEQITDAD
                     PDDCPVAPVGRWIVDPDGAVVRAGLVRHYAARHGLWQLDGDIAYLSGDQLPDGVRGFE
                     VLDEVGYSEKRLRQVLSAHDAGAVEILVRGVDVDPDVLRRRLKLRGSRQVSVVIARIG
                     SGTASRATAFICRPSR"
     gene            1426..2151
                     /locus_tag="VGP27_11740"
     CDS             1426..2151
                     /locus_tag="VGP27_11740"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV7852010.1"
                     /translation="MRISTSVLAATAAVLVGFSGVVAAGPASAAPPKCSDLQGGLVGQ
                     NCVIQAGDAGYSLQITYPADYPDVQAVFDWVKQTRDGFVNVAKSPDARTMPYQLEVTP
                     TEYNSAVPPRGTQSVVFKVFQDVGGAHPQTFFKAFNWDQTLRWPIVLDTGGKEKMQPL
                     FQPGANPWPVIFPLVQAELEKQMGTKPAIAPEVGLNPATYENFAIQNDSLVFFFGQGA
                     ILPESAGALTVTIPRAPVDRMIA"
     gene            complement(2209..3495)
                     /locus_tag="VGP27_11745"
     CDS             complement(2209..3495)
                     /locus_tag="VGP27_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV7852011.1"
                     /translation="MFRRLIVLASTALITGALAGCGNTDSWVDAHAADGWPAQYGDAA
                     NSSYTRVAGAEALELQWSRSVKGELGASVALGSGQYLAVNGQTPAGCSLMVWEADNNA
                     RQRWCTRLWQGGGSSSPLWDGFDNLYIGQPGAMLSFPPTQWIRWRQPVIGMPTTARIL
                     APNHLLVVTHLGQVLLFDAHRGSVIGNPLDLVEGVDPTDSMRGLTDCRPARPRCPVAS
                     APAFAAKSGVVVVPVWQPNAPASVLVGLKYNPDRNPKLSVAWTSDAVKAGTMASPVAS
                     ADGSTIYVNGRDDRLWAINAADGKPKWSTPLKFLSQTPPSVAPGDDVIAGGGPDTRLV
                     AIKDRGDHADVMWTRDDVSPLTTSSRAGEHVAYTVVKDGEDRQALLVFDPGDGHTVNS
                     YPLPDAQGWPVGVAIGHDRRVVVSTSAGQVYGFSPG"
     gene            3577..4314
                     /locus_tag="VGP27_11750"
     CDS             3577..4314
                     /locus_tag="VGP27_11750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV7852012.1"
                     /translation="MTTMWGAPLHKRWRGSRLRDPRQAKFLTMASLRWVISNRAYTPW
                     YLVRYFRLLKFKLANPHIITRGMVFLGKGVEIQATPELSTMEIGRWVHIGDKNTIRCH
                     EGSLRFGDKVVLGRDNVINTYLDIELGDSVLMADWVYLCDFDHRMDDINLPIKDQGIL
                     KGPVRIGPDTWIATKVTILRNTTIGKGCVLGSHAVVKGDIPDYSIAVGAPAKVVKNRK
                     LAWDTSAAERAKLAAALADIERKKASR"
     gene            complement(4368..4514)
                     /locus_tag="VGP27_11755"
     CDS             complement(4368..4514)
                     /locus_tag="VGP27_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852013.1"
                     /translation="MDTRRFTLATALTPVAAYLGLFVGLIDANAVLDCVHRLGILMPS
                     FILR"
     gene            complement(4558..>5206)
                     /locus_tag="VGP27_11760"
     CDS             complement(4558..>5206)
                     /locus_tag="VGP27_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7852014.1"
                     /translation="IDAGLWPFARRRPRQGPAQLLYLGRLEYEKGIHDAIAALPRIRR
                     THPGTTLMIAGDGTQYEWLVGQARKHKVLKAVTFVGRVDHKGLVQLLHSADAAVLPSH
                     YEPFGIVALEAAATGTPLVTSNVGGLGEAVINGQTGVSFPPRDIAALAAAVRAVLDDP
                     QAAQQHAVAARDRLTSDFDWHTVAGETAQVYLAAKRGVRQPHPRRLIVEHALPDR"
BASE COUNT          830 a         1730 c         1761 g          885 t
ORIGIN      
        1 gccggttggg ctggcactgg gccaagttcg cgttcggcag ttggatgacg ctgagctggg
       61 tggaccacaa cgtgtggcgt cgcgtcgtcc cgaagggctg gttctacaac gtcatggtga
      121 ccggggtcaa gccgtcgtag atttcggcct ggatgccgtc gcctatctga acagcgacgc
      181 cggagcgcag gcgctgcgcg atgtcgacgg ctaccggctg accgatgcga gccgcatctc
      241 cgacatcggt tcgatacgag tgcaattcgg cgagcacgcg cccgctctcg tggagacggt
      301 gctgctgcgc cgaagggcga gagccaaatt cgccgacccg tcggactggt tgttcaccga
      361 tgacgcgttg caacaggcca ccgcgcaacc ggtggccgaa caccgggcac ggcggctggc
      421 cggcgccacc gtgcacgacg tcacctgttc ggtcggcacg gagcttgccg cgctgcgaaa
      481 gtcggccggt tacgtgatgg gcagcgacat cgatccggtc cggctcgcca tggcccggca
      541 caatctcggt gacgtaccgc tgtgccgagc cgacgcactg cgacccgtca cccgcgacgc
      601 cgtggtgctc gccgacccgg cgcgcaggcg gtcggggcgg cggcggttcg gcctgctcga
      661 ctacacgcca ccgctggatc gtctgctcga cgtctaccgc caccgtgatg tcgtcgtaaa
      721 gtgtgcgccg ggaatcgatt tcgcccaact tgagcgcctc ggtttcaacg gtgaagtcga
      781 gatcacgtcg ctgggcggca gcgtgcgcga ggcgtgtctg tggtcgccgg gactggccgc
      841 gacgggcgtg cgccgtcggg ccaccatgtt ggacggtgcc gagcagatca ccgacgccga
      901 tcccgacgac tgcccggtcg caccggtggg ccggtggatc gtcgatcccg acggtgcggt
      961 cgttcgggcc ggactggtcc gccactatgc cgccaggcac gggctctggc agctggatgg
     1021 cgatatcgcc tacctgtccg gcgaccagct gcccgacggc gtgcggggct tcgaggtgct
     1081 cgacgaggtc ggctacagcg agaagcgact gcggcaggtg ctgtctgcgc acgacgcggg
     1141 cgccgtcgag attctggtgc gcggtgtcga cgtcgacccg gacgtgctgc ggcggcggct
     1201 gaagttgcgt ggctcgcggc aggtttcggt ggtgatcgcg cgtatcggct ccggcacggc
     1261 aagtcgggca acggcattca tctgccgacc ctcgcggtga cttaccgttc cccgactcgc
     1321 acccgctgcg gtcctcgttc gtactcgcct tcgggagctt gtctgtcgcc tgtattgtga
     1381 cgtcggcggc tacggtgccg ctctggctcg aagaggattt catcgatgcg catttccacg
     1441 tcggttctcg ctgccactgc ggccgtactc gtcggcttct cgggtgttgt cgcggctggg
     1501 cccgcgtcgg ccgcgccgcc gaagtgctcg gacctccagg gaggtctggt cgggcagaac
     1561 tgcgtgatcc aggcggggga cgccggttac tcgttgcaga tcacctatcc ggccgactat
     1621 ccggatgtgc aggcggtctt cgactgggtc aagcagacca gggacggctt cgtcaacgtg
     1681 gcgaagtcgc ccgacgcgcg caccatgccc taccagctgg aggtcacccc gaccgagtac
     1741 aactcggcgg ttccgccgcg cggcacgcag tcggtggtgt tcaaggtgtt ccaggacgtc
     1801 ggtggggccc acccgcagac cttcttcaag gcgttcaact gggaccagac ccttcgctgg
     1861 cccatcgtgc tggacaccgg gggcaaggag aagatgcagc cgctgttcca gccgggcgcc
     1921 aacccgtggc cggtgatctt cccgctggtg caggcggagc tggagaagca gatgggcacc
     1981 aagccggcga tcgcacccga ggtgggactg aacccggcga catacgagaa cttcgcgatc
     2041 cagaacgact cgctggtgtt cttcttcggc cagggcgcaa tcctgccgga gtcggcgggt
     2101 gccctgacgg tgacgatccc gcgggcgccg gtcgaccgga tgatcgccta gccgggcgac
     2161 gatcaagcgg cgaggaatga gccgcgttga ggaggccggc gaattggcct agccgggcga
     2221 gaagccgtac acctgcccgg cgctcgtaga aacgacgacg cggcggtcgt ggccaatcgc
     2281 caccccgacc ggccaccctt gggcatccgg cagcggatag ctgttgacgg tgtggccgtc
     2341 gcccgggtcg aacaccagca gcgcctggcg gtcctcgccg tctttgacca cggtgtaggc
     2401 gacgtgttcg ccggcccggc tggacgtcgt caagggggag acgtcgtctc tggtccacat
     2461 cacgtcggcg tggtcgccgc ggtccttgat ggcgaccagc cgcgtgtcgg ggccgccgcc
     2521 ggcgatgacg tcgtcgcccg gcgccaccga cggcggcgtc tgcgacagaa acttcagcgg
     2581 cgtcgaccac ttcggcttgc cgtcggcggc gttgatggcc cacagcctgt cgtcacggcc
     2641 gttgacgtaa atcgtcgacc cgtcggcaga tgccaccggg ctggccatcg tcccggcctt
     2701 gaccgcgtcg ctcgtccagg ccaccgacag cttggggttc cggtcggggt tgtatttcag
     2761 cccgacgagc accgaggccg gagcattggg ctgccagacc ggcaccacaa ccacgcccga
     2821 cttggcggcg aacgcgggcg cggaggcgac cgggcagcgc ggccgcgcgg gccggcagtc
     2881 ggtcagaccc cgcatcgagt ccgtcgggtc gacgccctcc accaggtcca gcggattgcc
     2941 gatcaccgag ccgcggtggg catcgaaaag cagaacctga cccaggtgcg tcacgaccag
     3001 aagatgattc ggcgccagaa tccttgcggt ggtgggcatt ccgatgaccg gctgccgcca
     3061 ccggatccac tgcgtcggcg ggaacgacaa catcgcaccg ggctgcccga tgtagaggtt
     3121 gtcgaagccg tcccacagcg ggctcgacga tccaccgccc tgccacagcc gggtgcacca
     3181 ccgttgccgg gcattgttgt cggcttccca caccatcagc gagcagccgg cgggggtctg
     3241 cccgttgact gccagatact gtcccgaccc caacgccacc gatgcgccga gctcaccctt
     3301 caccgagcgg ctccattgca gttcgagcgc ctcggcgcct gccacacggg tgtagctgct
     3361 gttggccgcg tcgccgtact gtgcgggcca gccgtcggcc gcgtgcgcgt cgacccagga
     3421 gtcggtgttg ccgcacccgg ccagagcacc cgtgatcagc gctgttgacg ccagcacgat
     3481 gagtcgccgg aacactcgat cctttcccgt ccctattcgt aggcccacgt gagggtaacc
     3541 gttcgggata cggaggctcg cgtaggcttt ccggccatga cgacgatgtg gggagcgccg
     3601 ctgcacaagc gctggcgggg atctcggctc cgcgacccgc gccaggccaa atttctcacg
     3661 atggcttcct tgaggtgggt gatctcgaat cgcgcctata cgccgtggta cctggtgcgc
     3721 tacttccggc tgctcaagtt caagctggcc aacccccaca tcatcaccag gggcatggtt
     3781 ttcctcggca aaggcgtgga gattcaggcg actcccgagc tgtcgacgat ggagatcggc
     3841 cgctgggtcc acatcggcga caagaacacc atccgctgcc acgagggttc gcttcgcttc
     3901 ggcgacaagg tggtgctggg ccgcgacaac gtcatcaaca cctacctcga catcgagctg
     3961 ggcgactcgg tgctgatggc cgactgggtc tacctctgcg acttcgatca ccggatggac
     4021 gacatcaact tgccgatcaa ggatcagggc atcctcaagg gtccggtccg catcggtccc
     4081 gacacctgga tcgcgacgaa agtcaccatc ctgcgcaaca ccacgatcgg gaagggctgc
     4141 gtgctcggct cgcacgcggt cgtcaagggc gatatcccgg actattcgat cgcggtcggc
     4201 gcaccggcca aggtcgtgaa gaaccgcaag ctggcgtggg acacgtctgc cgccgagcgt
     4261 gccaagctgg cggccgcact ggccgacatc gagcgcaaga aggcatcccg ctaactgctt
     4321 tcattgactc tgcgtccttg gtgacgatgc ccaagtagcc gaagccgtca tcgcagaatg
     4381 aacgacggca tcaggatgcc cagtcgatgc acgcagtcca acaccgcgtt ggcgtcgatc
     4441 aggccgacga agagtcccag gtaggcagcg accggggtca acgccgttgc gagggtgaac
     4501 cttctggtgt ccatatccgc acgttaaatg cggaatgatt cgacgccgat cgaattgtca
     4561 gcggtcgggc agggcgtgct ccacgatcag cctgcggggg tgcggttgac gcaccccacg
     4621 tttggcggcc aggtacacct gtgcggtttc ccctgcgacc gtgtgccagt cgaagtcgga
     4681 cgtcaaccga tcgcgggcgg cgaccgcatg ctgttgcgcg gcctgcggat cgtcgagcac
     4741 cgcgcgcacc gccgcagcga gggcggcgat gtcgcgcggt gggaacgaca ctccggtctg
     4801 cccgttgatc accgcctcgc ccagaccgcc gacgtttgac gtcaccaggg gcgtgcccgt
     4861 cgcagccgcc tccagcgcca cgatgccgaa tggctcatag tggctgggga gcaccgcggc
     4921 gtcggcgcta tgcagcaact gcaccaggcc tttgtggtca acccgtccga cgaacgttac
     4981 ggccttgagc accttgtgtt ttcgagcctg tccgacgagc cactcgtact gggtgccgtc
     5041 gcctgcgatc atcagtgtcg taccgggatg ggtacgcctg atgcgcggca gcgccgcgat
     5101 cgcgtcgtgg atacccttct cgtattccag cctgccgaga tagagcagct gcgcgggacc
     5161 ctgccgcggc cgtcgccgcg cgaacggcca caaacccgcg tcgatt
//
LOCUS       DASYLX010000483         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1228574,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000483 DASYLX010000000
VERSION     DASYLX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228574"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VGP27_11765"
     CDS             complement(<1..851)
                     /locus_tag="VGP27_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEV7852015.1"
                     /translation="MADTLLTESDWTARDRAYRRRVEEFLAPHQIRAQIGEPHPVWDF
                     LFRYYSLRPRRLRVWHPGFGVVLGGRATPAARHYVNRTGYGTHPAGVTVTGQYLRSRV
                     DTVQFIANLLRATDTRSPRMNCFGMHEWAMVYRAPAVRHERVPLRLGADGTDAVVESM
                     PLRCTHFDAFRFFTEPAAALNAGTPSREGQSGWEQPGCVHANMDLYKWAFKLGPLVES
                     GLVMDCLELAADARALDMKASPYDLRDYGFEPIAVETPAGRAAYARAQQDIAGRAAPL
                     RTRIADR"
     gene            complement(844..1896)
                     /locus_tag="VGP27_11770"
     CDS             complement(844..1896)
                     /locus_tag="VGP27_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV7852016.1"
                     /translation="MGDFFGVLLTVALLAANAFFVGAEFALISARRDRLEALAEQGKR
                     RAVTVIRAGQNLSVMLAGAQLGITICSILLGRVGEPAVAHLLEKPFDLFGVPDALLHT
                     VSFVIAITVVVVLHVLLGEMVPKNIAIAGPESAAMLLVPPYLAWVRLARPVIAVYDWC
                     AHMTLRAMGIKAKPELENTVSTVELSEMIAESLSEGLLDPEEHNRLSRALQIRTRAVA
                     DVAIPLSEVHAVPAAADDTGPTVDAIQQALQDSGYSRFPVTGSGGRYIGYIHIKDVLN
                     LVNDADAVVDLSMVRPLPQVPASSPLPDALSRLRRSNSHLALVTADDGSVTAMVALED
                     LVEDLVGAMRHRTHGG"
     gene            complement(1896..>2957)
                     /locus_tag="VGP27_11775"
     CDS             complement(1896..>2957)
                     /locus_tag="VGP27_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV7852017.1"
                     /translation="GLALALSLLIATSVSMVFGELVAQYLAVARPLETARIVAGPQVL
                     FSMMFTPVIKLTNGAANWILRRLGIEPAEELRSARSPQELGSLVRNSARRGALDPTTA
                     ALVDRSLQFGSRTAEELMTPRSKIEVLQADENVADLVVMAMETGYSRFPIVEGDLDET
                     IGLVHVKQVFEVPRDDRSRTALAGLATLVPVVPSTLDGDDVMAQVRASALQTVLVADE
                     YGGTAGMVTMEDLIEEIVGDVRDEHDDVTPDVLRMSRDSWRVSGLLRIDEVAQNGGFR
                     APEGEYETIGGLVMQELGHIPKPGEAVELTAFDPDGPIDDPVRWLATVVRMDGRRIDL
                     LELTRLGRISESHDEDGER"
BASE COUNT          492 a         1028 c          984 g          453 t
ORIGIN      
        1 cagcgatcgg cgatcctggt ccgcagcggc gccgccctgc cggcgatgtc ctgctgtgcg
       61 cgggcgtagg cggcccgacc ggcaggcgtt tcgacggcga tgggctcgaa accgtagtcg
      121 cggagatcat aggggctcgc cttcatgtcg agagcgcgag cgtcagcggc cagctccagg
      181 cagtccatca ccagacctga ctcgacgagc gggccgagtt tgaaagccca cttgtacaga
      241 tccatgttgg cgtgcacgca gccgggctgc tcccagcccg attggccttc gcgtgacggc
      301 gtgcccgcgt tgagtgcagc cgcaggctcg gtgaagaacc ggaacgcgtc gaagtgggtg
      361 cagcgcagcg gcatcgactc gacgacggca tcggtcccgt ccgcaccgag ccgcagcggc
      421 acacgttcgt gcctaaccgc cggcgcccgg tacaccatcg cccactcgtg cattccgaag
      481 cagttcatgc ggggcgagcg agtgtccgtc gcgcgcaaca ggtttgcgat gaactgcacg
      541 gtgtcgaccc gggaccgcag gtattggccg gtgaccgtca cgcccgcggg atgggttccg
      601 taaccggtgc ggttcacata gtgtcgcgcc gcgggggtgg cccgtccgcc gagcacgacg
      661 ccgaagccgg gatgccagac gcggagccgg cgcggccgca ggctgtagta gcgaaagagg
      721 aaatcccaca ccggatgtgg ctcaccaatc tgcgcgcgga tctgatgagg cgcgaggaac
      781 tcctcgaccc ggcgacggta ggctcggtcc cgcgccgtcc agtcggactc ggtcagcagc
      841 gtgtcagcca ccgtgggtcc gatgccgcat cgcgccgacg aggtcctcga ccagatcctc
      901 gagtgccacc atcgccgtca cgctgccatc gtcggcggtg accagggcga ggtgactgtt
      961 gctgcggcgc agccgcgaca gcgcgtcggg cagcggcgag gacgcaggca cttggggcag
     1021 cggccggacc atcgacagat cgacgaccgc gtcggcgtcg ttcaccagat tcagcacgtc
     1081 cttgatgtgg atgtagccga tgtagcgccc accgctgccg gtcaccggga accgcgagta
     1141 accgctgtcc tgcagcgcct gttggatcgc gtcgacggtg gggccggtgt cgtcggccgc
     1201 cgccggtacc gcgtgcacct cgctcagcgg tatggccacg tcggcgacgg cgcgggtgcg
     1261 gatctgcagt gcacgcgaca atcggttgtg ctcctccggg tcgagcagcc cctcggacag
     1321 cgactcggcg atcatctccg acaactcgac cgtcgaaacc gtgttctcca gttcgggttt
     1381 cgccttgatg cccatcgcac gaagcgtcat gtgcgcacac cagtcgtaga ccgcgatgac
     1441 gggtcgcgcc agccgaaccc aggccaggta cggcggaacg agcagcatgg ccgccgattc
     1501 gggtccagcg atcgcgatgt tcttcggcac catctcaccg agcagcacat ggagcaccac
     1561 gacgaccgtg attgcgatga cgaatgacac ggtgtgcagc agggcgtccg gcacgccgaa
     1621 gaggtcgaac ggcttctcga gcaggtgagc gaccgcgggc tcaccgaccc ggccgagcag
     1681 gatcgagcag atcgtgatgc cgagctgggc gcccgccagc atcaccgaca ggttctggcc
     1741 cgcccggatc accgtgaccg cgcggcgctt gccctgttcg gcgagggctt cgaggcggtc
     1801 acggcgcgcc gagatcagcg cgaactccgc cccgacgaag aaggcgttcg ccgcgagcag
     1861 cgcgacggtg agcaacacgc cgaagaagtc gcccatcagc gttcaccgtc ctcgtcgtgc
     1921 gactcgctga tgcggccgag cctggtcagc tcgagcagat cgatgcgcct gccgtccatc
     1981 cgcacgaccg ttgccagcca gcgcaccgga tcgtcgatcg ggccgtcggg gtcgaacgcc
     2041 gtcagctcga ccgcctcacc cggcttgggt atgtggccga gctcctgcat caccaggccg
     2101 ccgatggtct cgtattcgcc ttcgggggcg cggaatccgc cattctgggc cacctcgtcg
     2161 atgcgcaaga ggcccgatac ccgccagctg tcgcggctca tgcgcaacac gtcgggtgtg
     2221 acgtcgtcgt gctcatcacg gacgtcgccg acgatttcct cgatcagatc ttccatcgtc
     2281 accatgcccg cggtgccgcc gtattcgtcg gccaccagca cggtctgcag cgcactcgcc
     2341 cgcacctgcg ccatcacgtc gtccccgtca agcgtcgacg gcaccacggg aaccagggtg
     2401 gccaggcccg ccagcgcggt gcgcgaccga tcatcccgcg gcacctcgaa cacctgtttg
     2461 acgtgcacaa gaccgatcgt ctcgtccagg tcgccctcca cgatcgggaa gcgagaatag
     2521 ccggtctcca tcgccatcac gaccaggtcg gcgacgttct cgtcggcctg cagcacctcg
     2581 atcttggacc gcggcgtcat cagctcttct gcggtgcgcg acccgaactg cagcgagcgg
     2641 tccaccagtg ccgccgtggt cgggtctagg gcgccgcggc gcgcggagtt gcgcaccagt
     2701 gacccgagct cctgcggcga tcgggccgac cgcagctcct cggcaggctc gatgcccagc
     2761 cggcgcagga tccagttggc ggcgccgttc gtcagcttga tcaccggggt gaacatcatc
     2821 gagaacagca cctgcggtcc cgccacgatg cgcgccgtct ccaacggtct ggccacggcc
     2881 aggtattgcg ccaccagttc gccgaagacc atcgacaccg acgtggcgat cagcagggag
     2941 agcgcaagag ccaggcc
//
LOCUS       DASYLX010000484         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2107736,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000484 DASYLX010000000
VERSION     DASYLX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107736"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGP27_11780"
     CDS             <1..253
                     /locus_tag="VGP27_11780"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEV7852018.1"
                     /translation="DRIEQKAGGKTTPIGIVPTADDLDLSGLDVDAADVNEALAVNAA
                     EWREEIPLIEEWFEFVGEKLPTGIKDEFDALKHRLAESD"
     gene            355..1692
                     /locus_tag="VGP27_11785"
     CDS             355..1692
                     /locus_tag="VGP27_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7852019.1"
                     /translation="MIEVPETRYAKSGGVNIAYQVVGDGPFDLIHVPPFVSNLELQWE
                     DPAERRYFERLASFSRLIMFDKRGTGLSDRVAVATLEERMDDLRAVMDATGSDRAAVY
                     GSSEGGALSILFATTYPDRVSALALYGAYPRMAWAPDYPEGITEEVATPILQNMEMNW
                     GRGLAGGLPMWALTPDRVDDPVQQKAQGRWERLSASPGAAMALQQVIRELDIRDVLST
                     IRVPTLVVYRTADLVHAAGSRYLGAHIPGAKVVELSGSDYFPYLGDQDAILDEVEEFL
                     TGARPVHAPDRALATVLFTDIVSSTEHAAAVGDDAWTRVLDSHDNVIAREIDRHRGRR
                     IKTTGDGVLATFDGPARAVRCAQAISRAVRSLGIEVRAGLHTGEIELRGADIGGIGVH
                     IGQRVSALAGPGEVLVSSTVKDLVAGSGITFADHGSHVLKGVPDEWRVFAAGY"
     gene            1765..>2793
                     /locus_tag="VGP27_11790"
     CDS             1765..>2793
                     /locus_tag="VGP27_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7852020.1"
                     /translation="MQIREHAEAHPLKPAVVMHPSGMTVTFGELEARANRLAHFFRKH
                     GLREGDAVAILMENNEHYHAVMWAARRSGLYYVPINTHLTAAEAAYIIENSSAKAVVG
                     SAPLRKTLEGLQTELPNGLPQLLIIADGAERSATSASSDLDDWHRYPECVADEPDTPI
                     DDEIEGDLLQYSSGTTGRPKGIKRELQHVSPAEAPGMMSGLVSFWLDPEAIYLSPAPL
                     YHTAPSVWSMTIQGAGITTVVMDKFDAEGCLDAIQRHRVTHGQFVPVMFTRMLKLPED
                     VRNSYDLSSLKRVMHAAAPCPVEIKKQLIDWWGPIVDEYYASSEAIGSTLITADEWLA
                     HPGSVGRSM"
BASE COUNT          525 a          905 c          889 g          474 t
ORIGIN      
        1 cgaccgcatc gaacagaagg ccggcgggaa gacgacgccg atcggcatcg tgccgaccgc
       61 cgacgatctg gacctgtctg gcctggatgt cgacgccgcc gacgtcaacg aggcgctggc
      121 ggtcaacgct gccgagtggc gtgaggagat cccgctgatc gaggagtggt tcgagttcgt
      181 cggcgagaag ctgccgaccg gtatcaagga cgagttcgac gccctgaagc accgtctggc
      241 cgaatccgac taaccgcgag cgtgcgcgtc tgcagccaac acgccgcaac atttcagtag
      301 tgtgcgcacg ctcgttgtgc acaccggcgc ctgacagcga cctaaactgg cagcgtgatc
      361 gaggtccccg agacccggta cgccaagtca ggcggcgtca acatcgccta ccaagttgtg
      421 ggcgacggcc ctttcgacct cattcatgtt ccgccgttcg tgtcgaacct cgagctgcag
      481 tgggaagatc cggccgagcg gaggtacttt gagcgcctgg cttcgttcag tcgtctgatc
      541 atgttcgaca agcgcggcac cggcctgtcc gatcgcgtgg cggtggccac gctcgaggaa
      601 cggatggacg atcttcgtgc agtgatggac gcgaccggct cggaccgagc ggccgtctac
      661 ggcagctccg aagggggagc gctgtcaatc cttttcgcca cgacctatcc ggaccgcgta
      721 tcagcactcg ctctatacgg cgcctacccc cgcatggcat gggcgccgga ctaccccgag
      781 ggtattaccg aggaggtagc cactccgata ttgcaaaaca tggagatgaa ttggggccgc
      841 gggctcgcgg gaggactgcc aatgtgggcc ctcacaccgg accgggtcga cgatccagtt
      901 caacaaaagg cacagggtag atgggagcgt ctatcggcca gcccgggggc ggccatggcg
      961 ttgcagcaag tgattcgcga gctcgatata cgcgacgtct tgtcgacgat ccgcgtaccg
     1021 acactcgttg tctaccggac ggctgacttg gtacatgctg cgggcagcag atatctgggt
     1081 gcgcatatcc ctggggccaa agtcgtcgaa ctttccggca gtgactattt cccatatctc
     1141 ggagaccagg acgcgatcct cgacgaggtc gaggaattcc tcaccggcgc acgtcccgtg
     1201 cacgcgccgg accgcgcttt ggcgaccgtg ctcttcaccg acattgtctc gtcaaccgag
     1261 cacgcagccg ctgtcggcga cgacgcatgg acgagggtgt tggacagcca cgacaacgtg
     1321 atcgcccgtg agatcgaccg ccaccgaggt cgccggatca agacgacggg tgacggtgtg
     1381 ttggcgacct tcgacggacc cgcacgtgct gtgcgatgcg cgcaagcgat ctccagggct
     1441 gtgcggtcgt tgggaatcga agtgcgggcg ggacttcaca ccggcgagat cgagcttcgg
     1501 ggtgccgaca tcggtgggat cggcgtgcat atcggccagc gcgtctcagc gctcgccggc
     1561 ccgggcgagg tgctggtgtc atccaccgtc aaagacttgg tggccggctc gggtatcaca
     1621 ttcgccgacc atggatctca cgtgctgaag ggcgttcccg acgaatggcg agtattcgcc
     1681 gccggctact agtccgagca ttcgggacct gcgttttctg ccctcttgac acctgtcaag
     1741 ttcgggcgtc gtaaagtcgc agccatgcag atccgcgagc acgcagaagc gcaccccctc
     1801 aagcccgccg tcgtcatgca tccgtcgggt atgacggtga cgttcggcga gttggaggcc
     1861 cgcgccaacc ggctcgcaca cttcttccgc aagcacggcc ttcgcgaggg cgacgccgtc
     1921 gcgatcttga tggagaacaa cgagcactac cacgcggtga tgtgggcggc acggcgcagc
     1981 ggcctctact acgttccgat caacacccac ctgaccgcgg ccgaggcggc ctacatcatc
     2041 gagaacagca gcgccaaggc ggtcgtcggg tccgccccgc tacgaaagac actcgagggg
     2101 ctccagaccg aactgcccaa cgggctgccc cagctcctca tcatcgccga cggagccgaa
     2161 aggtcggcga catccgcctc cagcgacctc gatgactggc accgctaccc cgaatgcgtt
     2221 gccgacgaac cggatacgcc catcgatgac gagatcgaag gcgacctgct gcagtactcg
     2281 tcgggcacca cgggccgccc caagggcatc aagcgcgaac tccagcacgt gtcgccggcg
     2341 gaggcgccgg gcatgatgtc gggcctggtg agcttctggc tcgaccccga ggccatctac
     2401 ctcagccccg cgccgctcta tcacaccgcg ccctcggtgt ggtcaatgac gattcagggt
     2461 gcgggcatca cgacggtggt gatggacaaa ttcgacgccg aaggctgcct cgacgccatc
     2521 cagcgacacc gcgtcacgca cggccagttc gtcccggtga tgttcacccg catgctgaaa
     2581 ctacctgagg acgtgcgcaa ttcgtacgat ctgtccagcc tcaagcgggt catgcacgca
     2641 gcggcgccct gtccggtcga gatcaagaag cagctgatcg actggtgggg accgatcgtc
     2701 gacgagtact acgcctcctc cgaggcgatc ggctcgacac tgatcaccgc cgacgaatgg
     2761 ctggcccatc ccggttcggt cggcagatcg atg
//
LOCUS       DASYLX010000485         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2764482,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000485 DASYLX010000000
VERSION     DASYLX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764482"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGP27_11795"
     CDS             <1..217
                     /locus_tag="VGP27_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852021.1"
                     /translation="EAILGVWQRRSSEVAQTVVVGLYPSWDISEDALAAADRFLSGEM
                     QPSLRRLVVEGRAGVERALRARAFDIA"
     gene            complement(336..803)
                     /locus_tag="VGP27_11800"
     CDS             complement(336..803)
                     /locus_tag="VGP27_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 domain-containing protein"
                     /protein_id="HEV7852022.1"
                     /translation="MVSGEVATREPADLPRGWVVTTSGRISAVTEPGELSVTYPFPVK
                     DLVRIDDALKFGSRRAKARFAVYIGDLGADTAATARAVLAEVPTPNNAVLLAVSPNQK
                     AIEVVYGSELQGRGIEESAPLGVKAAAASFNYGNLIAGLVSAIKVMSAGVSPA"
     gene            complement(790..1026)
                     /locus_tag="VGP27_11805"
     CDS             complement(790..1026)
                     /locus_tag="VGP27_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852023.1"
                     /translation="MSTALAHSLLGGVPLAILLVLVLLIWGVGKNRKGPHPAAYKMSD
                     TEWTHAPILWAADEPESHGHAHPLTIGGGASGKW"
     gene            complement(1052..1693)
                     /locus_tag="VGP27_11810"
     CDS             complement(1052..1693)
                     /locus_tag="VGP27_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7852024.1"
                     /translation="MAHRKGHGHRHSGVAAGLSGAAPTPTLLHSVETHPPARDGSIWG
                     RRRVLLLNSTYEPLTALPMRRAVVMLICGKADVVHADPSGPVIHSASRSVAVPSVIRL
                     RSYVRVPYRARVPMTRAALMHRDRFRCAYCGSKADTVDHVVPRSRGGDHTWENCVAAC
                     STCNHRKADRLLAELGWTLRSTPTRPKGQHWRLLSTVKELDPAWMRYLGEGAA"
     gene            1826..>2147
                     /locus_tag="VGP27_11815"
     CDS             1826..>2147
                     /locus_tag="VGP27_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEV7852025.1"
                     /translation="MGDVTQSQRSFYDEVGGHDTFNAIVSRFYQLVREDEILRPLYPE
                     DDLDAAEVRLRMFLEQYWGGPRTYSDQRGHPRLRMRHSPFKIGFIERDAWLRCMHTAV
                     ASIDS"
BASE COUNT          347 a          722 c          737 g          341 t
ORIGIN      
        1 cgaggccatt ctcggcgtgt ggcagcggcg ctccagcgag gtggcgcaga cagtcgtcgt
       61 cggtctgtac ccgtcgtggg acatcagcga ggacgcgttg gccgccgccg accggttcct
      121 gagcggcgag atgcagccgt ccctgcgtcg gctggttgtc gaaggccgcg cgggcgtcga
      181 acgagcgctg cgggctcggg ctttcgatat cgcctagcgc gctattagcg cgatgacaga
      241 ttgcgcacgc tcgtgaccga cgaccgtgcg cggatgatga cccatgcccg gcgtgtcgcg
      301 cgcaaacacg cgcgctcgcg gtgtcggggg gacgatcagg ccggcgagac gccggcgctc
      361 atcaccttga tcgcgctgac gaggccggcg atcaggttgc cgtagttgaa cgacgccgcg
      421 gcggccttca cacccagtgg agcggactcc tcgatgccgc ggccctgcag ctccgagccg
      481 tagacgacct cgatggcctt ctggttcggc gaaaccgcga gcaacacagc gttgttcggc
      541 gtcggcacct cggcgaggac agcgcgcgcg gtcgcggcgg tgtcggcccc caggtcgccg
      601 atgtacaccg cgaaacgggc cttcgcccga cgcgacccga acttcagcgc gtcgtcgatc
      661 cgcaccaggt ccttgaccgg gaacggatag gtgaccgaca actcgccggg ctcggtgacg
      721 gcggagattc gtccgctggt cgtgacgacc cagccgcggg gcagatcggc cggttcgcgc
      781 gtcgcgactt caccacttac cactggcgcc gcctccaatc gtcaacggat gcgcatgccc
      841 atgcgactcg ggctcgtctg cagcccacag gatcggcgca tgagtccact cggtgtcgga
      901 catcttgtag gcggccgggt gcgggccctt gcggttcttg ccgacgcccc agatgagcag
      961 gaccaagacc aacaagattg ccagcggcac cccgcctagt agcgaatgtg caagtgcggt
     1021 gctcacgcgc aaaccctatc gcggcgactc gctaggccgc gccctcaccc aggtatcgca
     1081 tccatgcggg atcgagttcc ttcaccgtcg acagcagccg ccagtgctga cccttgggcc
     1141 gcgtcggggt cgagcgcagc gtccagccca gctcagccag cagccggtcg gccttgcgat
     1201 ggttgcacgt cgagcaggcg gccacacagt tctcccaggt gtggtcgccg ccgcggctac
     1261 gcggcaccac gtggtcgacg gtgtcggcct tgctgccgca gtaggcacag cggaaccggt
     1321 cgcggtgcat gagcgcggcc cgggtcatcg ggacgcgcgc ccggtagggc acccgcacgt
     1381 aggaacggag ccggatcacc gacggcacgg ccaccgaccg ggaggccgaa tggatcaccg
     1441 gcccggacgg gtcggcgtgt acgacgtcgg ccttgccgca aatcagcatg accacggccc
     1501 tgcgcatcgg cagggccgtc agaggttcgt atgtcgagtt caggagcaat acccggcggc
     1561 ggccccagat cgaaccgtcg cgagcaggag ggtgcgtttc gacgctgtgc agcagcgtcg
     1621 gggtgggcgc ggccccggat aagcccgccg cgaccccgga atgccggtgg ccgtggccct
     1681 tgcgatgcgc cattcgtcct ccgaccgaca gttcatcacg gattggggtc taccgcataa
     1741 caaattcgtt cgcgtcggct ggtcagcagg gtgaacttcc catgacatga ccgggcacgc
     1801 acttcggtgg gggcgagttg ggacaatggg tgacgtgaca cagtcccagc ggtccttcta
     1861 cgacgaagtg ggtggccacg acaccttcaa tgccatcgtg tcgcggttct accagctggt
     1921 ccgcgaggac gagatcctgc gtccgctgta ccccgaggac gatctcgacg ccgccgaggt
     1981 gcggctgcgg atgttcctcg agcagtactg gggtggcccg cgcacctact ccgatcagcg
     2041 cggccatcca cggctgcgga tgcggcacag cccgttcaag atcggtttca tcgagcgcga
     2101 cgcctggctg cggtgcatgc acaccgcggt ggcatcgatc gactcgc
//
LOCUS       DASYLX010000486         4582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_681957,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000486 DASYLX010000000
VERSION     DASYLX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681957"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGP27_11820"
     CDS             <1..273
                     /locus_tag="VGP27_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852026.1"
                     /translation="EPWEDLAVKLMLKWPNLYYSTSAFAPKYYPKAIIDYANSRGADK
                     VLYAGYFPMGLSLERIFGELPNVGLKDEVWPKFLYGNAARILGLKG"
     gene            280..2508
                     /locus_tag="VGP27_11825"
     CDS             280..2508
                     /locus_tag="VGP27_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7852027.1"
                     /translation="MVLATTTEQEHLADAVSRFAARHAPIEKTRASFDSIAAGESPHW
                     WGELTAHGFHAVHIAEGAGGQGGTLADLGCVIEAAAAALLPGPLLSTATASAVAILAG
                     ESAASLVADLVAGATAAVVLPEHSEVRAVRRQDGWRLTGSSASILGLCAAQRILLAAR
                     SEDNAERWFVLDTGRARGADFSIAQQRGTDLSTDVGVLNLVDHTVDARSELSGISTER
                     ARCIAAALTACASAGAVRRCADAATEYIRTREQFGRPVGAFQALQHKAAVLLVNAELA
                     AAAAWDAVRASAESLEQHRLAAGSAALMAVAAGPDLLLDALLMFGAIGYTWEHDTHLY
                     WRRAISLAASLGSTAHWSREVGEHSRTVKRSTALNLGDVESDFRASVATTLDHARALR
                     NDHPSDDPRAPGLATGAQRDMLAKAGLVAPHLPPPWGVGASAVQQVIINEEFDKRPDL
                     IKPSLGIAEWILPTILGSGTELQRERFAWPILGGTERWCQLFSEPGAGSDLASLTTRA
                     RKVDGGWLVNGHKIWTSQADRAQFGALLARTDPDASKHRGISYFLVDMASPGVIVEPI
                     KQASGHSDFNEVFLNDVFVADDMLVGTPGAGWDLAVATMAVERTAIGNYVNIDRAAAL
                     RHIAQIDGPDTDAALRALGEVEAYTTAIKAMVLRETLRLVEGQAAGPTSSIAKYAMVM
                     LLRRASTATLGLTGRIAMLEESDPAVVRPYFDMPSELIGGGTAEIQLTVIASMILGLP
                     RN"
     gene            2549..3742
                     /locus_tag="VGP27_11830"
     CDS             2549..3742
                     /locus_tag="VGP27_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852028.1"
                     /translation="MTTDLGFTYFDCDNHYYEALDAFTRHLEPKYKKRAIQWANIDGK
                     QRLIVGGRVNRFIPNPTFDPVGKPGALDEYFRGRNPKGADLNALFGDLEPIPPAYRDR
                     DARLALMDDQGMAGCIMLPTLGVGMEQALLPDLDAAVATFRAFNRWMDEDWGFAYKER
                     IFAAPYITLCKPASAVQELEWALERDARFIVMIPGPIATDVGMRAPGDPMFDDFWRLA
                     NDSGITVCYHSGETYYSKFMPAWGEPDYMMSFQALLGFRSLFSGDALQDTLANHLHNG
                     LFLRFPNLRMASIESGSTWVFHLFEKLTKSYGQIPFIYKEDPRETFKRHVWVSPFYED
                     ELASLLKLVGQTHIVMGSDYPHVEGLADPASYIKDLENFDYTPEQSRTVMRDNGIELS
                     IRRPV"
     gene            3779..4042
                     /locus_tag="VGP27_11835"
     CDS             3779..4042
                     /locus_tag="VGP27_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852029.1"
                     /translation="MVAKLLEVGEENPAVLLARSDLPDEIDTDHDLDALLAFGLEPDQ
                     VEEVARLAIGALEPDDVNTRRRRGEPAKTISRRWADLVDQWMT"
     gene            4199..>4582
                     /locus_tag="VGP27_11840"
     CDS             4199..>4582
                     /locus_tag="VGP27_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7852030.1"
                     /translation="MKFLLLTLITHQPDPVSGEKKSPAERLREVVDNAVIAEELGFDG
                     FAVGERHEDPFISSSPPVVLSNIAARTSEIGLFTAVTTLSLLDPVRAFEDYSTLDNLS
                     GGRLELIIGKGNGAAQADLFHVTTAD"
BASE COUNT          803 a         1515 c         1482 g          782 t
ORIGIN      
        1 gagccgtggg aggacctcgc cgtcaagctg atgttgaagt ggccgaacct gtactactcg
       61 acatcggcgt tcgcgcccaa gtactacccg aaggccatca tcgactacgc gaactcacgc
      121 ggcgcggaca aagtgctcta cgccggctac tttccgatgg ggctgtcgct cgaacggatc
      181 ttcggcgagc tgcccaacgt tgggctcaag gacgaggtct ggccgaagtt cctgtacggc
      241 aacgctgcac gaatcctcgg gctgaagggc tgacgcccta tggtgttggc gaccacgacc
      301 gagcaggaac acctggccga tgccgtcagc cggttcgcag cccgccatgc gccgatcgag
      361 aagacgcggg cctcgttcga ctccatcgca gcgggtgaat caccgcattg gtggggagaa
      421 ctgaccgcgc atggatttca tgcggtgcac attgccgaag gggccggcgg acagggcggt
      481 acgctcgccg atctcggctg cgtcatcgag gcggccgctg ccgcgttgtt gccggggccg
      541 ctgctgagca ccgcaactgc cagcgccgtg gcgatcttgg ccggcgagtc ggctgcgtcg
      601 ctggtcgccg atctcgttgc cggcgccacc gcagccgtcg tgctgccgga acactccgag
      661 gttcgtgccg tccgccggca ggacggctgg cggctcaccg gttcgtcggc atccatcctg
      721 ggactctgtg ccgcgcaacg cattctgctg gccgcgcgct ctgaggacaa cgccgaacgc
      781 tggttcgtgc tggacaccgg acgcgccaga ggcgcggact tctcgatcgc acagcaacgc
      841 ggcaccgact tgtcgacgga cgtgggcgta ctgaatctgg tcgaccacac cgtcgatgca
      901 cgctcggagt tgtccggaat ctccaccgag cgcgctcgct gcatcgcggc tgccctcacg
      961 gcgtgcgcat cggcgggtgc ggtccgacgg tgtgccgacg cggcgacgga atacatccgc
     1021 acccgcgaac agttcgggcg gccggtaggc gccttccagg ctctgcaaca caaggcagcg
     1081 gtactgctcg tgaacgccga gttggcggcc gccgccgcgt gggatgcggt gcgcgcttcc
     1141 gccgaatccc ttgagcagca tcggctcgct gccgggtcgg ccgcgctgat ggccgtggct
     1201 gccggccccg atctcctgct cgacgcgctg ctgatgttcg gtgcgatcgg gtacacatgg
     1261 gagcacgaca cccacctcta ctggcgacgc gccatcagcc tcgccgcgtc actcgggtcc
     1321 acggcgcatt ggagccgcga agtgggtgag cactcccgca ccgtgaagcg ctctaccgct
     1381 ttgaatctcg gcgacgtcga atccgacttc cgcgcgtcgg tcgcgacgac cctcgaccat
     1441 gcgcgggccc tgcgcaacga ccatccctcg gatgatccgc gcgcccccgg cctcgccacc
     1501 ggcgcccagc gcgacatgct ggcgaaggcg gggttggttg caccgcacct gccaccgccg
     1561 tggggcgtcg gcgcctcggc cgtgcagcag gtgatcatca acgaggagtt cgacaagcgt
     1621 cccgacttga tcaagccctc gctcggcatc gcggagtgga tcctgccgac gatcctcggc
     1681 agcggcacag aactgcagcg cgagcggttt gcgtggccga ttctgggcgg caccgagcgc
     1741 tggtgccagc tcttcagcga gccaggggcc ggctcggatc tcgcttcgct gactacacgg
     1801 gcccgcaagg tcgacggagg gtggctggtc aacgggcaca aaatctggac ctcgcaggcc
     1861 gaccgggcac aattcggcgc gctgctggcg cgtaccgacc cggatgcgtc caagcaccgc
     1921 gggatcagct acttcctggt cgacatggcg tcgccgggcg tcatcgtcga accgatcaag
     1981 caggccagcg gccactcgga cttcaacgag gtcttcctca acgacgtgtt cgttgcggac
     2041 gacatgctgg tcggaacacc gggcgccggt tgggatctgg ccgtggcgac gatggccgtt
     2101 gaacgcacgg cgatcggcaa ctacgtcaac atcgatcgcg cagccgcgct gcggcacatc
     2161 gcgcagatcg acggcccgga cacggacgct gcgctgcggg cactcggcga ggtcgaggcg
     2221 tacaccaccg ccatcaaggc catggtgcta cgcgaaaccc tgcgtttggt cgagggccag
     2281 gcggcggggc cgacctcgag catcgccaag tacgcgatgg tcatgctgct acgccgcgcg
     2341 tcgacggcga cgcttggcct caccggccgt atcgcgatgc tcgaagagtc ggatccggcc
     2401 gtcgtccggc cgtacttcga catgccatca gaactcatcg gcggaggtac ggcggagata
     2461 cagctgaccg tcatcgcatc gatgatcctc ggcctgccac gcaactagaa ccgacgacga
     2521 tttcatccat cgaaaggatg tggccgcaat gacgaccgac ttggggttca cctacttcga
     2581 ctgtgacaac cactattacg aggctttgga cgcgttcacc cgccacctcg agccgaagta
     2641 caagaagcgc gccatccagt gggcgaacat cgacggtaag cagcgcctga tcgtcggcgg
     2701 cagggtcaac aggttcattc cgaacccgac gttcgacccg gtgggtaaac ccggcgcact
     2761 cgacgaatac ttccgcggcc gcaatccgaa aggtgcggat ctgaacgcgc tgttcggcga
     2821 cctcgagccg attccaccgg cataccgtga ccgagatgcc cggctggcgc tgatggacga
     2881 tcagggcatg gccggctgca tcatgctgcc gacgctgggc gtcggaatgg aacaggcgct
     2941 gctgcccgac ctggacgccg ccgtcgcgac gttccgcgcc ttcaaccggt ggatggacga
     3001 ggactgggga ttcgcctaca aggagcgcat tttcgcggcg ccctacatca cgctgtgcaa
     3061 gcccgcgagc gccgtgcagg agctcgagtg ggcgctggaa cgcgacgcgc ggttcatcgt
     3121 catgattccc gggcccatcg cgaccgacgt cggcatgcgc gcgcccggcg acccgatgtt
     3181 cgacgacttc tggcggctgg ccaacgattc gggcatcacc gtctgctatc actccggtga
     3241 gacgtactac tcgaagttca tgcctgcgtg gggcgagccg gactacatga tgtccttcca
     3301 agcgctgctt ggcttccggt cgttgttctc cggggacgcg ctgcaggaca ccttagccaa
     3361 ccacctgcac aatggtctgt ttctgcgctt ccccaacctg cggatggctt cgatcgagag
     3421 cggctccacg tgggttttcc acctgttcga aaagctgacc aagtcctacg gccaaatccc
     3481 gttcatctac aaggaagacc cgcgcgagac cttcaaacgt cacgtctggg tgtcgccgtt
     3541 ctacgaggac gagctcgcca gccttctcaa gctggtgggt cagacgcaca tcgtgatggg
     3601 ttcggactac ccgcacgtcg aaggcctggc cgatcccgcg tcatacatca aggatctcga
     3661 gaacttcgac tacacacccg aacagagccg taccgtcatg cgcgacaacg gtatcgagtt
     3721 gtcgatccgc agaccggtct gatcgcgttc cgccacagtg aaagtcgaga agtccagctt
     3781 ggtcgccaag ctgttggagg tcggcgagga gaacccggcg gtgcttctcg cccgttccga
     3841 tctgcccgac gagatcgaca ccgaccatga tctcgacgcg ttgctcgcct tcggtctgga
     3901 gcccgaccag gttgaggaag tggcgcggtt ggcgatcggt gcgctcgaac ccgatgacgt
     3961 caacacccgc cgaaggcgcg gtgagccggc gaagaccatc tcccggcggt gggccgatct
     4021 cgtcgatcag tggatgacct agccgaggcc gggcggcagc ggtcgaattg cactcacggt
     4081 gattcattga cgacacgcgc ccgggggaat gattcggcct acgacgcggt tcgtctaggt
     4141 cgacacgacc gttccggagc aactggaggg caggcgtgac gacgacaccc ggtggccgat
     4201 gaagttcctg ttgctgacgc tgatcacgca ccagccggac ccggtgtccg gtgaaaagaa
     4261 gagcccggcc gagcgcttgc gcgaggtcgt ggacaacgcc gtaattgccg aggaactcgg
     4321 cttcgacggc ttcgcggtcg gcgaacgcca cgaggatccc ttcatctcgt cgtctccacc
     4381 tgtggtgctg agcaacatcg ctgcacgaac gtcggagatc ggcctgttca ccgcggtgac
     4441 aacgctcagt ctgctcgatc cggttcgggc attcgaggac tactccaccc tcgacaacct
     4501 gtcgggtggg cgactcgagc tgatcatcgg caagggcaac ggtgccgcgc aggccgacct
     4561 gttccacgtg accaccgccg at
//
LOCUS       DASYLX010000487         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1997823,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000487 DASYLX010000000
VERSION     DASYLX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997823"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..332)
                     /locus_tag="VGP27_11845"
     CDS             complement(60..332)
                     /locus_tag="VGP27_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852031.1"
                     /translation="MSAYEVLTIALIAVIGTAATAAIYLGLLNWMGAFYVVRCAGCHH
                     LAFSTANSAQASCPHCRHPVLTHPLYSLHHRRTGSEVRVVGDRLHY"
     gene            619..>2304
                     /gene="typA"
                     /locus_tag="VGP27_11850"
     CDS             619..>2304
                     /gene="typA"
                     /locus_tag="VGP27_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV7852032.1"
                     /translation="MDFRNVAIVAHVDHGKTTLVDAMLRQSGALSHRGDDAIERLMDS
                     GDLEKEKGITILAKNTAVHRQHPDGSMTVINVIDTPGHADFGGEVERGLSMVDGVLLL
                     VDASEGPLPQTRFVLRKALAAHLPVILVVNKTDRPDARIAEVVSESHDLLLDVASDLD
                     DDAQKAAEEALGLPTLYASGRAGIASTVEPANGENPEGENLDPLFDVLLEHIPPPQGD
                     PEAPLQALVTNLDASAFLGRLALIRIYKGRIRKGQQVAWMREVDGHPVITNAKITELL
                     VTEGVDRTSTDEAIAGDIVAVAGLPEIMIGDTLADTDHAHALPRITVDEPAISVTIGT
                     NTSPLAGKVSGHKLTARMVKSRLDAELVGNVSIKVVDIDRPDAWEVQGRGELALAVLV
                     EQMRREGFELTVGKPQVVTRTIDGKLHEPFEAMTIDCPEEYVGAITQLMAARKGRMEE
                     MTNHAAGWVRMDFIVPSRGLIGFRTDFLTETRGTGIANAVFDGYRPWAGEIRSRHTGS
                     LVSDRTGTITPFALTQLSDRGTFFVDPGEDTYAGQVVGINPRPEDLDLNVTREK"
BASE COUNT          422 a          733 c          762 g          387 t
ORIGIN      
        1 aacgtcgatt tcgcggcatc gcgggacagc ggaggatgct gctgtcctgc cctagccggt
       61 cagtagtgca gccggtcacc caccacgcga acctcgctgc cggtgcgccg gtggtgcaag
      121 gagtagagcg gatgcgtgag caccgggtga cggcagtgcg ggcaggacgc ctgcgcacta
      181 ttggccgtcg agaacgccag gtggtgacag ccggcgcagc ggacgacgta gaaggcgccc
      241 atccagttca gtaaccccag gtagatcgcc gcggttgccg ccgtcccgat caccgcgatc
      301 agagcaatcg tcagaacctc ataagccgac atatccgcca ccttcgcgct cgcctgccgg
      361 gtagcaccac ggtacgcctg aatacgaggg atgatcagga gttccgggcc cgctcgaaca
      421 ccttcgtcag ctgcttgccc cgaatgccgt tgcgctcggc cctgcgcacc tgatgcacca
      481 caacggggca tttcttgcag cgcggcctgc tgcgacagca ctttttgttc ggtttcaggt
      541 cggtcagctt gctcggcttc aagtgacggg catctctcgt tttcgtaatc ggccgtctgc
      601 actgcgacaa tcaccagcgt ggatttccgg aatgtcgcca tcgtggcaca cgtcgaccat
      661 gggaagacga ccctcgtcga cgcgatgctg cggcagtcgg gcgcgctgag ccatcgcggc
      721 gacgacgcga tcgagcgcct gatggattcc ggtgacctgg agaaggaaaa gggcatcacg
      781 atcctggcga agaacaccgc cgtgcaccgt cagcaccccg acggcagcat gacggtgatc
      841 aacgtgatcg acacacccgg tcacgctgac ttcggcggtg aggtggaacg cgggctgtcg
      901 atggtggacg gggtgctgct gctcgtcgat gcgtccgaag gcccattgcc gcagacccgg
      961 ttcgtgctgc gcaaggccct ggccgcgcat ttgcctgtga ttctggtggt gaacaagacc
     1021 gaccggcccg atgcccgtat cgccgaggtg gtgtcggaga gtcacgactt gctgctcgac
     1081 gtcgcctccg acctcgacga cgacgcgcag aaggctgccg aggaggcact cggactgccg
     1141 acgctttatg cgtcgggacg cgccggcatt gccagcactg ttgaacccgc caacggcgaa
     1201 aatcctgaag gcgagaacct cgatccgctg ttcgacgtgc ttctcgagca catcccgccg
     1261 ccgcagggtg atccggaagc cccgctgcag gcgttggtca ccaacctcga cgcgtcggcg
     1321 ttcctgggca ggctcgcgct gatccgcatc tacaaggggc ggattcggaa gggccaacag
     1381 gtcgcttgga tgcgtgaggt ggacggtcac cccgtcatca ccaacgccaa gatcaccgag
     1441 ctacttgtca ccgagggcgt ggatcggaca tcgacggatg aagccatcgc tggggacatc
     1501 gttgccgtgg cgggtctgcc ggagatcatg atcggcgaca cccttgccga caccgatcac
     1561 gcgcacgcat tgccacggat caccgtcgac gagcccgcca tctccgtcac gattggcacg
     1621 aacacgtcgc cgcttgcggg caaggtctcg gggcacaagc tgaccgcccg gatggtgaag
     1681 tcgcggttgg acgccgagct cgtcggcaac gtgtcgatca aggtcgtcga catcgaccga
     1741 cccgatgcgt gggaggtgca gggccgaggc gagctggcgc tggccgttct cgtcgagcag
     1801 atgcgccgcg agggattcga gctgacggtc ggcaagccgc aggtcgtcac gcgcacgatc
     1861 gacggaaagc tgcatgagcc gttcgaagct atgaccatcg actgcccgga ggaatacgtg
     1921 ggcgccatca ctcagttgat ggcggcgcgc aagggccgca tggaggagat gacaaaccac
     1981 gccgcgggtt gggtacgaat ggatttcatc gtcccgagcc gcgggctgat cgggttccgc
     2041 accgacttcc tcaccgagac ccgcggcacg ggtatcgcca acgcggtgtt cgacggctac
     2101 cggccgtggg cgggggagat ccggtcgcgg cacacgggct cgttggtgtc cgaccggacc
     2161 ggcaccatca cgccgttcgc gctgactcaa ctgtccgacc gcggcacgtt cttcgtcgat
     2221 ccgggggagg acacctacgc tggccaggtt gtcgggatca atccgcgacc ggaggatctc
     2281 gacctcaacg tcacgcgaga gaag
//
LOCUS       DASYLX010000488         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3095476,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000488 DASYLX010000000
VERSION     DASYLX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095476"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..790
                     /locus_tag="VGP27_11855"
     CDS             2..790
                     /locus_tag="VGP27_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7852033.1"
                     /translation="MTLDRPEAANAQTAGILKDLDDAWRRADEDPEVRVIVLMTTGKH
                     FSAGHDMTGVDPSADGRSLGPRRSDGKLIAEDYYDWETRGYLDYARRWRDIPKPSIAA
                     VQGKCIAAGLMLCWPCDLIVAADNAQFSDPVGLMGIGGVEFHAHTWELGARKAKEMLF
                     TAGSVTAEEALRSGMVNHVVPLDELRSATMALAHRVAQTDPWALRMAKRAVNHTMDTM
                     GFSTAIASCFDMHHLGHTRALAATGGQTVVMADLERMKAAGRGK"
     gene            complement(1428..3170)
                     /locus_tag="VGP27_11860"
     CDS             complement(1428..3170)
                     /locus_tag="VGP27_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852034.1"
                     /translation="MTYDAIIRNGRWFDGTGAPSAIRNIGIRDGRVVAVTPQNLDESD
                     CPTILDATDRWVLPGMVDIHTHYDVEVLGGPSLSESLRHGVTTVFVGSCSLSTVHVGP
                     HDAGDLFGRVEAIPREHVICAIDAYKTWSSGREYIAALEALPLGPNVAAFIGHSDMRA
                     ATMGLDRATRSRERPTETELAQMERMLTEALDAGFLGMSSMQLRFDKLDGEICRSRTL
                     PSTYAKARELRRLKSVLRRTGRVLQTAPDIKNPLNVASQLAQSLGIMGKPLATSLLSA
                     ADIKANPHAIRVLGPAARLVNLFGGDFRWQHLPVPFEVYADGIDLVVFEEFGSGAAAL
                     HLRNHMERNALIRDEGYRRRFRKEYESKFGVKVWHRDFFDADIVGCSDDSVIGKSFGQ
                     VGVDRGGLHPVDAFLDLVVEHGAALRWRTTISNHRPDVLKRLAREPGVQMGFSDAGAH
                     LRNMAFYNFGLRLLRHVYDAQKAGAPFMTVEHAVHRLTGELADWYHVDAGHLRIGDRA
                     DLLVLDPAHLDASVDAYAEQRVAQYDGLSRMVNRNDDTVRAVFVAGRAVFLNGEATDI
                     LGRERTGSFLRVTG"
     gene            complement(3209..>4259)
                     /locus_tag="VGP27_11865"
     CDS             complement(3209..>4259)
                     /locus_tag="VGP27_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEV7852035.1"
                     /translation="SDDLVLVTTSGTTALPKSVAHTHGSLVRHAYILARHRAVTSADR
                     IYSPMPFFWVGGLTMVVLQALSTGAAILTQDVFDAGSTLALLERERATLISCWAQASQ
                     AMVDHPDFSKRDLTSVRGGTMLEALPPDRRPSEPGLTPNMLGMTETGGPHTMVEVPDT
                     PLPTERRGSFGIPLPGVVAHRIIDMAGLEAAPGQEGEIQVRGQILMSGIYKKERHEVF
                     SPDGWYATGDRGFFDAAGHLHFTGRASALIKTAGSNVSPAEVESVLDAVPGVLHSFVV
                     GLPHSVRGQVVGAVVVPSHGARLSVEAVAAHARTHLSTFKVPAVIKVVAETDLPMLPT
                     GKVDRQRLVGLLTAD"
BASE COUNT          747 a         1380 c         1405 g          727 t
ORIGIN      
        1 catcaccctc gaccgtcccg aggcggcgaa cgcccagaca gcgggcatcc tcaaagacct
       61 cgacgatgcg tggcgccgcg ccgacgagga ccccgaggtc cgggtcatcg tcctgatgac
      121 cacgggcaag catttctccg ccgggcacga catgacgggc gtcgatccgt cggcggacgg
      181 gcgctccctg ggtcctcggc gcagcgacgg caagctcatc gccgaggatt actacgactg
      241 ggagacccgt ggctacctgg actacgcccg gcgctggcgc gacatcccca agccgtccat
      301 cgcggcggtg cagggcaagt gcatcgccgc cgggctgatg ctgtgctggc cctgcgatct
      361 gattgtcgcc gcggacaacg cgcagttctc cgacccggtc ggcctgatgg gcatcggggg
      421 cgtcgaattc cacgctcaca cgtgggaact gggcgcccgc aaggccaagg agatgctgtt
      481 caccgccggc tccgtgacgg ccgaggaggc gctgcgctcc gggatggtca accacgtcgt
      541 accgctcgac gaattgcgtt cggccacgat ggcgctcgcg catcgcgtcg cccagaccga
      601 cccctgggcg ctgcggatgg ccaagcgggc cgtcaaccac accatggaca ccatggggtt
      661 ctcgacggcg atcgcctcgt gtttcgacat gcaccatctt gggcacaccc gggcactggc
      721 ggcgaccggt gggcagacag tggtgatggc cgacctggaa cggatgaagg ccgccggccg
      781 cggcaagtga gcacccgcac ctacaagttc gggccccggc cttaggttct actgtcaggg
      841 gtgcgcccgt cggctccgat gaacttccca agccgacgca cagtggggcc gatcaggacg
      901 cctgtgacca gcgaccagga cgcgcgcaca ataggcgcag gcccggccgg gtcaaccgat
      961 gtcgaccagg tcaactcggt ggattcaccg tcgagtgctc gcatccgcca acgctctgcg
     1021 aaacccgtca tggccggtag tgagcacctg tcgatacgaa acacgaactc ttcgttgatg
     1081 ttccaaccca agacggtctc ttcgaatgaa gagccgatcg ccctcatcgt gcgcctgctt
     1141 cccacgccgt gcggtggtgc tgatatccat gcgcttgcag tggagaaccc cggcatccag
     1201 tgtccccaac cactggggtc acacgctacg gcggcgaaga cagacccgac cggggcatgg
     1261 accactcctc tgcgatggat gcgaatcggt gcgcaatcta tgaagtcggc ctcaactgcg
     1321 ttgagagtct tcatgaccga cctttcgggt tgtgttggac cgtctcgggt cgtgtgctca
     1381 cttcgcggcg acggtggtgt cgtcgcctgg gattccgatg ttaatcctca accggttacg
     1441 cgcagaaagc tcccggtgcg ctccctgccg aggatgtcag tggcttctcc gttcaggaat
     1501 actgcgcgcc cggcgacgaa gacggctctg acggtgtcat cgttgcgatt gaccattcgg
     1561 gacaggccgt cgtactgggc gacacgctgc tcggcgtagg cgtccacgga tgcgtcgagg
     1621 tgcgccggat cgaggaccag caggtccgcc cggtcaccga tgcgtaggtg tccggcgtcg
     1681 acgtggtacc agtcggcaag ctcgccggtc agacgatgga ctgcgtgctc caccgtcatg
     1741 aacggcgcgc ccgccttctg cgcgtcataa acgtggcgca ggagccggag cccgaagttg
     1801 tagaaggcca tatttcgcag gtgggctccg gcgtcggaga agcccatctg gacgcccggc
     1861 tcccgggcca gacgtttgag cacgtcgggg cggtgattgg agatcgtcgt gcgccagcgc
     1921 aacgccgctc cgtgttcgac caccagatcc aggaacgcgt cgacggggtg caatcctccg
     1981 cgatcgacac cgacctgccc gaatgactta ccgataaccg agtcatcgga acatcccacg
     2041 atgtcggcgt cgaaaaagtc gcggtgccat accttcacgc cgaacttgct ctcgtattcc
     2101 ttgcggaagc gccgtcggta gccctcgtcg cgtatcaaag cgttgcgctc catgtggttc
     2161 cgcaggtgca gcgccgctgc gccggaaccg aactcctcga acacgaccaa gtcaatgccg
     2221 tcggcgtaca cctcgaacgg caccggcagg tgctgccacc ggaagtcgcc gccgaacagg
     2281 ttgaccagcc gtgcggccgg gccaaggact ctgatcgcat gggggttggc cttgatgtcg
     2341 gccgctgaga gcaggctggt cgcgagcggc ttgcccatga tgcccagtga ctgcgcgagc
     2401 tgggaggcga cgttgagtgg atttttgatg tccggtgcgg tttgcagaac tcgacccgtc
     2461 cggcgcagta cagatttgag tcgccgcaat tctcgcgcct tggcatacgt cgacggcaag
     2521 gtgcgcgagc ggcagatctc gccgtcgagc ttgtcgaacc gcagctgcat cgacgacatc
     2581 cccagaaatc ccgcatccag ggcctcggtg agcattcgct ccatctgcgc caactcggtc
     2641 tcggttgggc gttcacgact gcgagtggcg cggtcgagcc ccattgttgc ggcgcgcata
     2701 tccgagtgcc cgatgaacgc cgcaacgttc ggtcccaagg gcagtgcctc cagcgcggcg
     2761 atgtactccc gaccgctgga ccacgtctta taggcatcga ttgcgcagat gacgtgctca
     2821 cgcgggatag cctcgacgcg accgaacagg tcgccggcgt cgtgggggcc gacatgaacc
     2881 gtcgataacg agcacgaacc gacgaagacc gtcgtcacgc cgtgccgcag cgattccgac
     2941 agcgagggac ccccgagaac ttcgacgtcg taatgggtgt gaatgtccac cattccgggc
     3001 aacacccacc gatcggtcgc gtccaggatc gtcgggcagt ccgattcgtc caggttttgg
     3061 ggcgtcaccg cgaccacgcg accgtcgcgg atgccgatgt tgcggatcgc cgacggcgcg
     3121 cccgtgccgt cgaaccagcg gccattccgg atgatggcgt cgtaggtcat gcggcggctc
     3181 tgtgggtcga ttgcgttctc cgataacatc aatcggctgt cagtagcccg acaagtcttt
     3241 ggcggtcgac cttgccggtg ggcagcatcg gtaggtcggt ttcggcgaca accttgatca
     3301 cggccggcac cttgaacgtc gacagatggg tgcgagcgtg tgccgcgacg gcctcgaccg
     3361 acaaacgtgc gccgtgagat ggcacgacga ccgcgcccac cacctgaccc cgcacggaat
     3421 ggggcaggcc gactacgaaa ctgtgcagca ccccgggcac cgcatcgagg accgactcga
     3481 cctcggccgg tgagacattg gaccctgcgg ttttgatgag cgcgctggcg cgcccggtga
     3541 agtgcagatg tcctgcggca tcgaagaatc cgcggtctcc agtggcatac cagccgtcag
     3601 gactgaacac ctcgtggcgt tccttcttgt agatgccgct catcaggatc tggccgcgta
     3661 cctggatctc gccttcctga cccggcgctg cctcgaggcc tgccatgtcg atgatccggt
     3721 gcgcaaccac gcccggtagc gggatcccga aggaaccgcg ccgttcggtc ggcagcgggg
     3781 tgtcgggaac ttcgaccatg gtgtgggggc cgccggtctc ggtcatgccg agcatattgg
     3841 gcgtcaggcc cggctcgctc gggcgccgat cgggcggcaa agcctccagc atcgtgccgc
     3901 cccggacgct ggtcaggtcg cgcttggaga agtcggggtg atcgaccatg gcctgactgg
     3961 cctgcgccca gcaggagatg agggtggccc gctcccgttc gagcaacgcg agcgtggagc
     4021 cggcgtcgaa cacgtcctga gtcaggatcg ccgcgcctgt cgacagggcc tgcagcacca
     4081 ccatggtcag gccgccaacc cagaagaacg gcatcgggga gtaaatccgg tcggcggagg
     4141 tgacggcgcg atgccgcgca aggatgtagg catggcgcac caggctgccg tgggtatgcg
     4201 caacggattt cggcagcgcc gtggttccgg acgtggtgac gaggacgaga tcgtcggag
//
LOCUS       DASYLX010000489         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_132868,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000489 DASYLX010000000
VERSION     DASYLX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132868"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..432
                     /locus_tag="VGP27_11870"
                     /pseudo
     CDS             1..432
                     /locus_tag="VGP27_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            502..1071
                     /locus_tag="VGP27_11875"
     CDS             502..1071
                     /locus_tag="VGP27_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV7852036.1"
                     /translation="MTERSELWQSAFELIRTRGYEHRDEPFKLASGQLSHDYIDGKYA
                     VDTGERLTVVSRAVAALAADNGIEFDAVGGLTMGADPLAHGVAMVTGKAWFSVRKEQK
                     QRGREQWVEGTRLTQGTRVLLVDDVISTGGSTALAFDRIAPLGVVVTGVIPMVDRGDV
                     AATLFAERDVPFVALVTYRDLEIEPIKPV"
     gene            1159..>2211
                     /gene="lysA"
                     /locus_tag="VGP27_11880"
     CDS             1159..>2211
                     /gene="lysA"
                     /locus_tag="VGP27_11880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV7852037.1"
                     /translation="MTLLDHVPSLRRAATPRIDPRIWPTTASVDALGRLCVGGVPLTE
                     IADEFRTPAYVLDEADFRARLQRYRSALPGVEVVYAAKSLLCTGVVRWVTESGAGLDV
                     SSAGELATALVAGADPARIVMHGNAKSQDELRDAAVIGVGRIVVDSLMDIAYLGCEAR
                     QPLRVLVRVTPDVDAHGHAAVMTGVNDQKFGFTLGHGHAEEAVKRIMAHRLLTLVGLH
                     CHIGSQITDPALFGEAIRRMVATMADIRTRHGVVLSELNIGGGHGVPYVSGDPELDPG
                     VLSELIDDALDEACAGERFPRPRIVVEPGRAISARAGVTLYRVISVKSQAGGRTFVAV
                     DGGMSDNPRVSLYGARY"
BASE COUNT          348 a          754 c          753 g          356 t
ORIGIN      
        1 gcgggcacgc tggcgacgct ctcctcggcc ggtgagccgt gggcctcgtt cgtcacctac
       61 gggttgctgg atggagcgcc ggtgctgtgc gtctcgaacc tcgccgagca cggccgcaac
      121 cttctcggcg atcaccgagc cagcatctcg attgtggcgc ccggcccgga cccggacccg
      181 cagtcgtggg gacgggtgac actcgccggt tgcgtcgaac gaccggtcgg cgacgaactc
      241 gccgccgcga gggacgcgca tctggccgcg gttccgggtg cgcagaacta catcgacttc
      301 ggcgacttcg ccctgtgggt gctgcgggtg cagcgggtgc gctggtatgg cggctacggc
      361 cggatggacc tggtcacggg cgatgcctac tcggcggccg caccccaccc gatctcgccg
      421 cgctcggcgg ggaccgctac ggacccgatt tgatttacgc tcggccacaa tagaattgac
      481 gcagcgcgtt aagatcggcc ggtgaccgag cgctccgagc tgtggcagtc cgcattcgaa
      541 ctgatacgca cccgcggcta cgagcatcgc gacgagccgt tcaagctggc gagcggccag
      601 ctcagccacg actacatcga cggcaagtac gccgtcgaca ccggtgagcg actgactgtc
      661 gtaagccgcg cggttgccgc gctcgcggcc gacaacggca tcgagttcga cgcggtaggc
      721 gggctcacca tgggcgccga cccgctggca cacggcgtcg cgatggtgac cggcaaggcg
      781 tggttctcgg tgcgcaagga gcagaagcag cgcggacgcg agcaatgggt cgagggcacc
      841 cggctgacgc agggcacccg cgtgctgctg gtcgacgatg tcatcagcac cggcggctcg
      901 accgcgctgg cattcgaccg catcgccccg ctcggcgtcg tcgtcaccgg cgtgatcccg
      961 atggtcgacc gcggcgacgt cgccgccacg ctgttcgccg agcgcgacgt cccgttcgtc
     1021 gcgctggtca cctaccgcga cctcgaaatc gagccgatca agccggtctg agcgtcaaca
     1081 cgtaaggatc cgtgcgcaca gcgtcaagac gccgtcaaga gttcccccgg cggtgcgcat
     1141 cgcgcgcacc gtggcgatat gacactgctg gaccatgttc cgtctctccg ccgggccgcc
     1201 acgccgcgga tagatccgcg aatctggccg acgacggcct ccgtggacgc cctcggtcgc
     1261 ctctgcgtcg gtggcgtgcc cctgaccgag atcgctgacg agttccgcac ccccgcctat
     1321 gttctcgacg aggccgactt ccgggcgcgg ctgcagcgtt accgctcggc tctgcccgga
     1381 gtcgaagtcg tctacgccgc gaagtcgctt ctgtgcaccg gcgtcgtgcg gtgggtcacc
     1441 gaatcgggag cgggcctcga cgtcagctcg gcgggcgaac tggcgaccgc gttggtcgca
     1501 ggtgcggacc cggcgcgcat cgtcatgcat ggaaacgcga agtcgcagga cgaactgcgc
     1561 gatgccgcgg tgatcggggt cggtcgcatc gtcgtcgact cgctcatgga catcgcctat
     1621 ctgggctgtg aggcgcgcca accccttcgg gtcctcgttc gcgtcacccc cgacgtcgac
     1681 gcccatggtc acgccgcggt gatgacaggg gtgaacgacc agaagttcgg tttcacgctc
     1741 ggccacggcc acgccgaaga agcggtgaaa cgcattatgg ctcaccgact tctgacactg
     1801 gtgggactgc actgccacat cgggtcgcag atcaccgatc ccgcgttgtt cggcgaggcc
     1861 attcgaagga tggtcgccac gatggccgac atccgcacca ggcacggcgt cgtgctgtcc
     1921 gaactaaaca tcggcggagg tcacggggtg ccctacgtga gcggcgatcc cgaactcgat
     1981 cccggcgtgc tgtccgaact catcgacgat gcgctcgacg aagcctgtgc gggcgaacgg
     2041 ttcccacggc cgcgcatcgt cgtcgaacct ggccgggcga tcagcgcaag ggccggagtc
     2101 acgctgtacc gcgtcatctc ggtcaagtcg caggccggtg ggcgcacgtt cgtcgcggtg
     2161 gacggcggta tgtccgacaa tccgcgcgtc agcctgtacg gcgcgagata c
//
LOCUS       DASYLX010000490         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3533084,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000490 DASYLX010000000
VERSION     DASYLX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533084"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VGP27_11885"
     CDS             <1..740
                     /locus_tag="VGP27_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852038.1"
                     /translation="GVPLEDRNKLFDWTNQMIGGDDPEFAEYDSLGATGELIWYAMQL
                     AQRKAEEPGEDIVSTLIDSEADGQLTESEFGMFVVTLTVAGNETTRNSITQGMMAFTD
                     HPDQWELFKAQRPKSAADEIIRWASPITAFQRTALVDTELDGVQIKKGQRLVLFYRSA
                     NFDEDVFDDPYAFDILRDPNPHLGFGGTGAHYCVGANLARMTIDLMFNAIADHIPELT
                     PVSAPERLRSSFINGIKHWQVEYRGPY"
     gene            904..1188
                     /locus_tag="VGP27_11890"
     CDS             904..1188
                     /locus_tag="VGP27_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7852039.1"
                     /translation="MPTYRFSCVACGDFAVVRPMAESGASAPCPECGRAAGRMFGSPA
                     LHAVDERLRRAYGASERSADAPQVVSSVPGQSRRATRITSDPRHARLPRP"
     gene            1203..>1634
                     /locus_tag="VGP27_11895"
     CDS             1203..>1634
                     /locus_tag="VGP27_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV7852040.1"
                     /translation="MPDVVFSVDQARSMRDQAVPGHNRWHPGVPPAANVRPGQHVRVE
                     CREWTDGQIGNNDSANDVRDVDLTHAHMLSGPLYVEGAEPGDLLVIDILDLGPVPQEV
                     GDAPGQGWGYTGIFAKANGGGFLTDQFPDAYKAIWDFAGQKC"
BASE COUNT          307 a          537 c          514 g          276 t
ORIGIN      
        1 tcggagttcc gctggaggac cgcaacaagc tgttcgactg gaccaaccag atgatcggcg
       61 gcgacgaccc cgagttcgcg gaatacgact cactgggcgc aacaggcgag ttgatctggt
      121 acgccatgca attggcgcaa cgcaaggccg aggagcccgg cgaggacatc gtcagcacat
      181 tgatcgactc cgaagccgac gggcagctga ccgagtccga gttcgggatg ttcgtcgtca
      241 ccctgacggt ggccggcaac gagacgaccc gcaactcgat cacccagggc atgatggcct
      301 tcaccgacca tcccgaccag tgggagttgt tcaaggcgca gcggccgaag agcgcggccg
      361 acgagatcat ccgctgggca tcccccatca ccgctttcca gcgcaccgca ttggtcgaca
      421 ccgagctcga cggtgtgcag atcaagaagg gccagcggct ggtgctgttc taccggtcgg
      481 cgaacttcga cgaggacgtc ttcgacgacc cctacgcgtt cgacattctg cgggatccca
      541 acccgcacct ggggtttggc ggcaccggcg cgcactactg cgtcggcgcc aacctcgcca
      601 ggatgaccat cgacctgatg ttcaacgcga tcgccgacca cattcccgag ctgacacccg
      661 tctccgcacc agagcgcctg cggtcgagct tcatcaacgg gatcaaacac tggcaggtcg
      721 aataccgggg cccgtactga gggatttcgg cgcgctaatc gactctgagc gcacgcgagc
      781 gcgccgaaat cgctgaaggt cgagatgaca actcggcatc ggtcactact atggccatag
      841 atgccgcagc gccgcagctt cagcacatgt agccgtgtag cacgggtctc acggaggtag
      901 gccttgccga cttatcgatt ttcctgtgtt gcgtgcggcg acttcgccgt cgtgcgtccg
      961 atggctgaat cgggggcatc cgcaccgtgc ccggagtgtg gccgagccgc cggccgaatg
     1021 ttcggttcac ccgcattgca cgcagtcgat gaacggctgc ggcgggccta tggggcgagc
     1081 gaacgcagcg ccgacgcgcc gcaggtcgtg tcatcggtgc ccgggcagtc gcgacgagct
     1141 acccgcatca cgtcggaccc gcggcacgcc cggctgcctc gtccctgaat ctggaggttg
     1201 tcatgcccga cgtcgtattt tcggttgatc aggcacgttc gatgcgcgat caggctgtgc
     1261 caggtcacaa ccggtggcac cccggtgtcc caccggcagc caatgtccgc ccgggacagc
     1321 atgttcgggt ggagtgccgg gaatggacag acggtcagat cggcaacaac gactccgcga
     1381 acgatgttcg cgacgtcgac ctgacacacg cccacatgct gtcaggcccg ctctacgtgg
     1441 aaggcgccga gcctggcgac ctcctcgtca tcgacatcct cgacctcggc cccgtgccgc
     1501 aggaggttgg cgacgctcca ggtcagggct ggggctacac cgggatcttc gccaaggcca
     1561 acggcggcgg ctttctcacc gaccagtttc ctgacgccta caaggcgatc tgggatttcg
     1621 caggccagaa atgc
//
LOCUS       DASYLX010000491         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1449064,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000491 DASYLX010000000
VERSION     DASYLX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449064"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VGP27_11900"
     CDS             complement(<1..676)
                     /locus_tag="VGP27_11900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV7852041.1"
                     /translation="MTEPISNSARLAAQPIAGGGTPVDMWTRWGRRLAPLNFDHCPAL
                     ILVAAHPDDETLGLGATAAMLAHRGIAVQVVAVTDGEAAYQEDPHGREKLAQARRDEL
                     ITASACLRLPRPIFLGIPDGEVAANEDRLEVELEAVLAESQPGAWCAATWRGDGHPDH
                     EATGRAAAAASKDVPAVFIEYPVWMWHWALPDDPAVSWDNARRVSLTSRDLATKTEAL
                     QCFSSQL"
     gene            735..1427
                     /locus_tag="VGP27_11905"
     CDS             735..1427
                     /locus_tag="VGP27_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV7852042.1"
                     /translation="MPVRAVLFDYSGTLFRLEEDDSWFHGMQVDEREVDAHVQAELMR
                     RLTAPTGRSVDITAEAYEAWVNRDLEPHLHREAYLHVLRKSGLPDHHAEALYERVIDP
                     TSWTAYPDTADVLKRLHRKGIKTAVVSNIAFDVRPAFDAIGAADDVDEFVLSYEVGAI
                     KPSPAIFQTTLTRLGVAPEYAVMVGDSEEADGGARTLGCGFLLVDPLPTTERPNGLRI
                     ALTGHGVELDSQ"
     gene            1437..1898
                     /locus_tag="VGP27_11910"
     CDS             1437..1898
                     /locus_tag="VGP27_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7852043.1"
                     /translation="MSDGERVRPPRWLKPVNKVFVLMLRLGVPVSRAESPVVLTVPGR
                     KTGKPRSTPVTPMGIDGKRYVVNGYPGADWVTNVRSVGEVTLTQGRRSERVRMVELSP
                     EEARPVLRAFPAAVPAGVELMKRVGLLTEGTPEEAERLAGRCPVFRIDPIT"
     gene            1908..2363
                     /locus_tag="VGP27_11915"
     CDS             1908..2363
                     /locus_tag="VGP27_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7852044.1"
                     /translation="MSNSKRIQPPWWLKYVNKAMIGVQRLGIGFGGKGPVILTVRGRK
                     SGKLRSTPITPMSVDGNRYVLAGLPGGDWAANARAAGEVELRQGRATERVRMVELPPD
                     EARPLLRLFPVEVPTGVSFLKNAGLVTGPDPDEYEALAGRCPVFRVDPV"
     gene            2428..>2567
                     /locus_tag="VGP27_11920"
     CDS             2428..>2567
                     /locus_tag="VGP27_11920"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101246.1"
                     /note="catalyzes the formation of
                     1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEV7852045.1"
                     /translation="MSSANPFDPDMWQPVDDFELTDITYHRHIVDGKPQPTVRVAFDR
                     PEV"
BASE COUNT          424 a          868 c          868 g          407 t
ORIGIN      
        1 ccaactggct ggaaaaacat tgcagtgcct cggttttcgt cgcaagatcc cgcgacgtca
       61 gcgatacccg tcgggcgttg tcccaggaca ccgcgggatc gtctggcaga gcccagtgcc
      121 acatccatac cggatattcg atgaacaccg ccgggacgtc cttcgaagcc gccgcggcgg
      181 cccggccggt cgcctcgtga tcggggtggc cgtcgccacg ccacgtcgcc gcacaccagg
      241 cccccggttg ggattcggcc agcaccgcct ccaattcgac ctcgagccgg tcctcgttgg
      301 ccgccacctc cccgtcgggt atgccgagga agatgggcct cggcaggcgg agacatgccg
      361 aggccgtgat gagctcgtcg cgccgggcct gcgccagctt ctcgcggccg tggggatctt
      421 cctgatacgc cgcctcaccg tcggtcaccg cgaccacttg caccgcgatg ccgcgatggg
      481 cgagcatcgc ggcggtggcc ccgagtccaa gggtttcgtc atcggggtga gccgcaacca
      541 ggatcagtgc cgggcagtga tcgaagttca gcggcgccag ccgcctgccc cacctggtcc
      601 acatgtcgac cggggtgccg cccccggcaa tgggttgcgc cgccaatcga gcggagttgc
      661 tgatcggctc ggtcaccgcc agtgagtaac cgatcgcatg gattccacac cccgcggcca
      721 tgcaaggatc agctgtgccc gtacgcgccg tgcttttcga ctactccggc acactgttcc
      781 gtctcgaaga ggacgacagc tggtttcacg gcatgcaggt cgacgaacgc gaagtcgacg
      841 cgcacgtgca ggccgagctg atgcgccggc tcaccgcacc gaccggtcgt tccgtcgaca
      901 taaccgcgga agcctatgaa gcgtgggtga accgcgacct cgaaccgcat ctgcaccgcg
      961 aggcgtacct gcatgtgctg cgtaaatcgg ggctgcccga ccatcacgcc gaggcgctct
     1021 acgaaagggt gatcgacccg acctcgtgga ccgcctaccc ggacaccgcc gatgtgctca
     1081 agcgtctgca ccgcaaaggc atcaagaccg cggtggtctc caacatcgcc ttcgacgtcc
     1141 ggccggcgtt cgacgcgatc ggggcggccg acgacgtcga cgagttcgta ttgtcttacg
     1201 aggtcggggc gatcaagccg agcccggcga tcttccagac cacgctgacg cggttgggcg
     1261 tcgcgcccga gtatgcggtg atggtcggtg acagcgaaga ggccgacggt ggcgccagga
     1321 cgctcggctg cggattcctg ctcgtcgacc cgttgcccac aacggagcgc cccaacggct
     1381 tgcgcatcgc gctgaccggc cacggggtcg aactggactc gcagtagcgt cgggccatgt
     1441 ccgacggcga gcgcgtccgg ccaccgcggt ggctcaagcc ggtcaacaag gtgttcgtgc
     1501 tgatgctgcg actcggcgta cccgtctcgc gcgccgagtc tcctgtggtg ctgaccgtgc
     1561 ccggccgcaa gaccggcaag ccgcggtcga cgcccgtcac gcccatgggc atcgacggca
     1621 agcgctacgt cgtcaacggc tatcccggcg ccgactgggt gaccaacgtc cgctcggtcg
     1681 gtgaggtgac gttgacgcag ggccgacgca gcgagcgggt tcgaatggtg gagctgtcgc
     1741 cagaggaggc acgcccggtg ctgcgcgcgt ttcccgccgc ggtgccggcc ggcgtcgaac
     1801 tcatgaagcg cgtcggactg ttgaccgagg gcacgcccga ggaggccgag agactggcgg
     1861 gccgctgccc ggtgttccgc atcgacccca tcacgtagcg tcgaatcgtg tccaacagca
     1921 agcgcattca gccgccgtgg tggctgaagt acgtcaacaa agcgatgatc ggggtgcagc
     1981 ggctcggcat cgggttcggc ggaaagggac cggtgatcct gacggtgcgg ggccgcaagt
     2041 cgggcaagct gcgctcgacg cccatcacgc cgatgtccgt cgacggcaat cgttacgtgc
     2101 tcgccgggct accgggaggc gactgggccg ctaacgcgcg tgctgcgggc gaagtcgaac
     2161 tgcgtcaggg ccgcgcgacc gaacgcgttc ggatggttga gcttccgccc gatgaggcgc
     2221 gaccgctgct gcggttgttc ccggtcgagg tgccgaccgg ggtgagcttt ctgaagaacg
     2281 cgggtttggt gacgggaccc gaccccgacg agtacgaggc cctggcgggc cgctgccctg
     2341 tcttccgcgt cgaccccgta tgacgactca tccgggattg ccgtagcggc gttccgacga
     2401 gcgaatgcag cacctagagt cgagcggatg agctccgcca acccgttcga tccggacatg
     2461 tggcagccgg tcgacgactt cgagctgacc gacatcacct accaccgtca catcgtcgac
     2521 gggaaaccgc agcccaccgt gcgggtggcg ttcgaccggc ccgaagt
//
LOCUS       DASYLX010000492         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1229090,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000492 DASYLX010000000
VERSION     DASYLX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229090"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1979
                     /locus_tag="VGP27_11925"
     CDS             348..1979
                     /locus_tag="VGP27_11925"
                     /inference="COORDINATES: protein motif:HMM:NF039734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HEV7852046.1"
                     /translation="MSDALLDKQAVSDVLKDKNIIGIGTVKPMAEPEVLRAIEEWLGG
                     WYIVDHSACGNANLPATHGDTWGFINGRAAWQKKNFVYGNADVWTDDFKSGSDHYAAS
                     NLPAGFDGVDSVVVGYIATHGVTSGTTFTMSAGGTGHGGCTVRSTQMSFGQNDLRYMF
                     FSTCQSVRNVDPGAVWFRTAKGIRAILGYHTNIVDSDAYGKYFFENWKKTGAKTTDSF
                     LDASWRVSHDQVPVAAWFGPDAATAESLRDNEEFFQLDAISGRSIAWSWYDARTLDRE
                     LLLSRVPIMTLEFNAPREPEGAAEVVSRLSSGEPVEIDETTISGDNVCHRTRNGSVLV
                     FNTRSGSIDLTFPAFSSPSVVDFTDAEAVNAATDYLRGREATLQGPRVDDADIALIPS
                     VVRRSMVGSAEESGSTEPHVRHVTVVFRQTVNGIATIGTGGVVEVTINGDREVCRVRS
                     VLREIAAVTAEDSAFDVATLQQRAEELAVAEVLAQPYVDECAVVKSEFGLFSADESIA
                     QSSAEPSFRVLVEMQTGPFSRLVEKIYPARQLSGN"
     gene            complement(2008..3393)
                     /locus_tag="VGP27_11930"
     CDS             complement(2008..3393)
                     /locus_tag="VGP27_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7852047.1"
                     /translation="MTTTDIAPEISTDLAAKANRHLWGHFARHGNGITPPIITRGEGV
                     TIWDDKGKSYIDGLSGLFVVQVGHGRKELAEAAAKQAETLSFFPLWSYATPPAIELSE
                     RIAHYAPGDLNRVFFTTGGGEAVETAWKLAKNYFKLTGKPGKYKVVSRAIAYHGTPQG
                     ALAITGLPAFKAPFEPLTPGGFRVPNTNFYRAPEPYHTDIKAFGEYCADRIAEAIEFE
                     GPDTVAAVFLEPVQNAGGCFPPPPGYFERVREICDEYDVLLVSDEVICAYGRIGSMFA
                     CDDFGYVPDMITCAKGLTSGYSPIGAMIASDRLFEPFNDGKTTFGHGYTFGGHPVSSA
                     VALANLDIFEREGINDHVKESAPAFRATLEKLYDLPIVGDVRGEGFFYGIELVKDKAT
                     KETFNDDESERLLRGFLTPALFEAGLYCRADDRGDPVVQLAPPLISGQKEFDAIYDIL
                     HNVLGEASRML"
     gene            complement(3390..3905)
                     /locus_tag="VGP27_11935"
     CDS             complement(3390..3905)
                     /locus_tag="VGP27_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV7852048.1"
                     /translation="MANPGADGIQPVFQRVQTRPGAAVQLDDLSKAIIEKLQQDGRRS
                     YAGIGKAVGLSEAAVRQRVQRMVDAGVMQIVAVTDPMQLGFARQAMIGIRCTGDTTRV
                     ADKLAAIDAVEYVVLTAGSFDAIIEVVCEDDDDLLDLLNTQIRALPGVISTETLVYLK
                     LVKQQYNWGTR"
     gene            4027..>4281
                     /locus_tag="VGP27_11940"
     CDS             4027..>4281
                     /locus_tag="VGP27_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7852049.1"
                     /translation="MTATVSASVAGSWINGAAVVTGGSRHQVVNPANGDVVAELALAT
                     PHDVDAAVAAARAAQRGWGSATPVDRATVLAKLAALMSDHA"
BASE COUNT          776 a         1317 c         1404 g          784 t
ORIGIN      
        1 gtagcgcgtt gggacgcggt cgtgaaggtc gccatcgtga ccgcagatta ggcgattcgg
       61 cgcgcgaatc aggggagcca cgctgtgact gatgtggttg gagttcccaa agtgactccc
      121 tcgggcacgc ccaaactgac atttgggcgg aaaagtgcga gacgagcgcg cccgaacgtc
      181 gatctcggtg ccgggcgcct ggtggctgca ggtcgaggct ggccagatcg gtgcgtcccc
      241 gcggagtcat cggcaagtgg cgtagccggc tactcgtgtc aggccacgca cgccgttctt
      301 acagtcgccc cgacgtcgta ttacccgaat cgtcacagga ggcgaccatg tcggatgctc
      361 tgttggataa gcaagccgtg tcggatgttc tgaaagacaa gaacatcatc ggcatcggaa
      421 cggtcaaacc tatggccgaa cccgaagtgc tgcgcgcgat agaagaatgg ctcggcgggt
      481 ggtacatcgt cgatcacagt gcctgtggaa acgccaatct gcctgcgacg cacggggaca
      541 catggggatt catcaacggc cgcgctgcct ggcagaagaa gaacttcgta tacgggaatg
      601 ctgacgtctg gaccgacgac ttcaagagcg gctccgacca ctacgcggca agcaaccttc
      661 ctgcaggttt cgacggcgtg gattccgttg tggtcggcta catcgctacc cacggcgtca
      721 cgtcgggcac caccttcacg atgagcgcgg gtgggaccgg ccacggcgga tgcactgtca
      781 ggtcaaccca aatgtccttc gggcaaaacg atttacgcta catgttcttc tccacctgtc
      841 agtcggtgag gaatgtcgac cctggtgctg tctggttccg taccgccaag ggaatacgcg
      901 cgattctcgg ttaccatacg aacatcgtcg attccgacgc gtacggtaag tacttctttg
      961 agaactggaa gaagaccgga gccaagacga ccgactcgtt tctcgatgcg agctggcgtg
     1021 tcagccatga ccaggtgccg gtggcggcgt ggttcggtcc cgacgcggca accgcggaaa
     1081 gcctgcggga taacgaggaa ttctttcagc tcgacgccat ctcgggcaga tcgatcgctt
     1141 ggagctggta tgacgcgaga acgctggacc gggaactgct gctgtcgagg gtgccgatca
     1201 tgacgctcga gttcaatgca ccgagagaac ctgagggcgc cgcagaagtg gtctcccgcc
     1261 tgagctcagg tgaaccagtg gaaatcgacg agacgacaat cagcggtgac aacgtctgcc
     1321 atcgcacccg caacggttct gtgctggtct tcaacacccg ttccggcagc atcgatctca
     1381 cgttcccggc cttctcatcg ccgtctgtcg tcgacttcac agatgccgag gcggtgaacg
     1441 ctgccacgga ttatctgcgc gggagggaag cgacgttgca ggggccgcgg gtggacgacg
     1501 cagacatcgc cctgatcccg tcggttgtcc gccgttcaat ggtcgggagc gctgaggagt
     1561 cgggcagcac agaaccgcat gtacgacacg tgacggtcgt attccggcag acggtcaacg
     1621 gcatcgcaac gatcggcacc ggcggcgtcg tggaggtcac gatcaacggc gaccgggaag
     1681 tgtgccgggt gagaagcgtg ctgcgcgaga tcgcggcggt caccgccgag gactcggcgt
     1741 tcgacgtcgc gacgttacaa cagcgggcag aggagctggc ggttgccgag gttctcgcgc
     1801 agccgtacgt ggacgaatgc gcggttgtga agtcggaatt cgggctcttc tcggcagacg
     1861 agagcatcgc tcagagcagc gcggagccgt cgttccgcgt actggtggag atgcagacgg
     1921 ggccgttctc ccggctcgtc gagaagatct acccggcccg acagctcagc ggtaactagc
     1981 cggttcggtt gccgacaggt ccgtcgacta cagcatcctg ctggcttcgc cgagcacgtt
     2041 gtgcaggatg tcgtagatcg cgtcgaactc cttttgcccg ctgatcagcg ggggagccag
     2101 ctgcacgact gggtcgccgc ggtcgtcggc gcggcagtac agacccgcct cgaacagcgc
     2161 gggcgtcagg aagccgcgga gcaggcgctc ggattcgtcg tcgttgaagg tctccttggt
     2221 ggccttgtcc ttgaccaact cgatgccgta gaagaagccc tcgccgcgca catcgccgac
     2281 gatcggtagg tcgtacagct tctccagcgt cgcccggaac gcgggcgcgc tctccttgac
     2341 gtggtcgttg atgccctcgc gctcgaagat gtcgaggttg gccagcgcca ccgccgacga
     2401 caccggatgt ccgccgaacg tgtagccgtg cccgaacgtg gtcttgccgt cgttgaacgg
     2461 ctcgaacagc ctgtcgctgg cgatcattgc gccgatcggc gagtaacccg acgtcaggcc
     2521 cttggcgcag gtgatcatgt cgggcacgta gccgaagtca tcacacgcga acatcgagcc
     2581 gatccggccg taggcgcaga tgacctcgtc ggacaccagc aggacgtcgt actcgtcgca
     2641 tatctcgcgg acccgctcga aatatccggg cggcggcggg aaacaaccgc ctgcgttctg
     2701 caccggctcg aggaacaccg cagcgacggt gtcggggccc tcgaactcga tggcctcggc
     2761 gatgcggtcc gcgcagtact caccgaatgc cttgatgtcg gtgtggtacg gctcgggggc
     2821 gcggtagaag tttgtgttcg gcacgcggaa cccgcccggt gtcagcggct cgaacggcgc
     2881 cttgaacgcc ggaaggccgg tgatggccag tgcgccctgg ggcgtgccgt ggtaggcgat
     2941 cgcacgggag accactttgt acttgcccgg tttgccggtc agcttgaaat agttcttcgc
     3001 cagcttccac gcggtctcga cggcctcgcc gccaccggtg gtgaagaaca cccggttcag
     3061 atcgcccggc gcgtaatgcg cgatccgctc ggacaattcg atggcaggcg gtgtggcata
     3121 ggaccacagc gggaagaagg acagcgtctc ggcctgcttg gccgccgcct cggcgagctc
     3181 cttacggccg tggccgacct gcaccacgaa caggcccgac agcccgtcga tgtagctctt
     3241 gcccttgtca tcccagatcg tgacgccctc gccacgagtg atgatgggcg gcgtgatgcc
     3301 gttgccgtgc cgtgcgaaat ggccccacag gtggcggttc gcctttgccg ccaggtcggt
     3361 tgaaatttct ggtgcgatgt cagtcgtcgt catcttgttc cccaattgta ttgctgttta
     3421 acgagtttca ggtatacgag cgtttcggtg gatatcactc ccggcagtgc gcgaatctgg
     3481 gtgttcagca gatcgagcag atcgtcgtca tcctcgcaga ccacttcgat gatggcgtcg
     3541 aaggaacctg cggtgagcac cacatactcg accgcgtcga tcgcggccaa cttgtcggcc
     3601 acccgtgtcg tgtctccggt gcagcggatg ccgatcatcg cctgtcgggc gaatccgagc
     3661 tgcatcggat cggtaaccgc aacgatctgc atgacgcctg cgtcgaccat gcgctggacc
     3721 cgctgccgca ccgcggcctc ggagagtccg accgccttgc cgatgcccgc atacgatcga
     3781 cgaccgtcct gctgcagctt ttcgatgatc gccttggaca ggtcgtcgag ctggactgcc
     3841 gctccaggac gcgtttggac gcgctggaat acgggttgga tgccatcagc acccgggttg
     3901 gccatgcctg cgattgtgca cggaatccgt cgtcaagagc aaccaatcca atgaaatcac
     3961 gcgccaagct tcccgtcact gcaggaatgc gtagcccgtg gcaaccccgt cggctagcgt
     4021 tgggccatga ctgcgacggt atcggcaagc gtggccggca gctggatcaa cggcgcggcg
     4081 gtggtcaccg gcggctcccg tcaccaggtc gtcaacccgg cgaacggcga cgtcgtcgcg
     4141 gagctggcgc tggccacccc gcacgatgtc gacgccgccg tggcggcggc gcgcgcggcg
     4201 caacggggct ggggaagtgc caccccggtc gaccgcgcga ccgtgctggc caagctggct
     4261 gcgctgatgt ccgaccacgc c
//
LOCUS       DASYLX010000493         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3862560,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000493 DASYLX010000000
VERSION     DASYLX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3862560"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGP27_11945"
     CDS             <1..739
                     /locus_tag="VGP27_11945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7852050.1"
                     /translation="TGPDGNWLEPLVFLTAVAAKTTRIRLATAVLLAALRRPAVLAKQ
                     LATMDVLSGGRVDLGVGVGWQREEYEAVGLAFEDRGRLLDHTLEVSRLLWTQQRARYD
                     SPELSFDGIHQMPKPVQPGGIPIWISGTVNKRVARRLSNFGTRWIPWGPAISNLAESV
                     PAMKKAIADVGGDPTDLAVQGAAALKRDADGGIDVGASLAPVEKQVAAGATDIRFSGS
                     LPKDLHQAQQQLSQLVAAFRGIAENSV"
     gene            867..1316
                     /locus_tag="VGP27_11950"
     CDS             867..1316
                     /locus_tag="VGP27_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852051.1"
                     /translation="MKLKNTAVTATLLGAMSAAAVGLGAGLAQAEPFPAPPPWPVPAP
                     AEPGIGVQGPNVSTPGVDFQTPGADINGPSVSAPGLGIDGPDASIGGPSVNVPGVDLQ
                     GPAASVAPPAWAPPQPPPPPWAPTRPVSWNAEAQAWGVYTDAGFQPV"
     gene            complement(1362..1838)
                     /locus_tag="VGP27_11955"
     CDS             complement(1362..1838)
                     /locus_tag="VGP27_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852052.1"
                     /translation="MRLTRLTKPALRGAAVFGAVVAAVALASPAWAYQEFQISMDTCN
                     ADNQSGDTCVYTTVANNNDGLTVQFVSSPDMCSDIIAHISTDAGGGMRELGADRVSPG
                     HGGKQYGIPKPANGGKYSTISVHANGIEGGCNTGGLLMWSGTLIAENTGYALPPKP"
     gene            complement(2012..>2468)
                     /locus_tag="VGP27_11960"
     CDS             complement(2012..>2468)
                     /locus_tag="VGP27_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEV7852053.1"
                     /translation="EQAGADGLWVTEIDPDLADTAAFCEHYGIGLDASANCVVVEAKR
                     ADRVWHAACMVLATTRADVNGIVRKHVDARKISFASMDTAVSLTGMASGGITPVGLPA
                     DWPILVDRNVADQQQVIIGSGIRGSKLLAATSVVAALPAAEVLAITKDA"
BASE COUNT          403 a          777 c          845 g          443 t
ORIGIN      
        1 gacaggtcct gacggcaatt ggcttgagcc gcttgtcttc ttgaccgcag tggcagccaa
       61 gaccacgcgt atccggctgg caaccgcggt gttgctcgcg gctctcaggc gtccggcagt
      121 gctggccaag cagttggcga cgatggacgt cctgtccggc ggtcgcgtcg acctaggtgt
      181 cggagtcggc tggcagcgcg aagagtacga agcggtcggc ttggcgttcg aggatcgtgg
      241 acgtctgctg gaccacaccc tggaggtcag ccgactcctg tggacccaac agcgcgcccg
      301 ttacgactca ccggaattgt cgttcgacgg catccaccag atgccgaaac cggtgcagcc
      361 cggcggcata cccatctgga tcagtggcac cgtcaacaag agggtggcgc gccggttgag
      421 caacttcggg acgcgctgga tcccttgggg tccggcgatc agcaacctcg cggagtccgt
      481 ccccgcgatg aagaaggcaa tcgccgacgt ggggggcgac ccaaccgatc tggctgtgca
      541 gggcgccgcc gctttgaaga gggatgccga cggcgggatc gacgtgggcg catccctcgc
      601 cccggtggag aagcaggtgg cggcgggcgc caccgacatc cgcttcagtg gatcgctacc
      661 aaaggacctg caccaggcac aacagcagtt gtcgcagctt gtcgcagcgt tccgcgggat
      721 cgctgagaac tctgtttagc tctccgccgt ccgtgggtag gccacgaggg cggggggtca
      781 taggccgtcc atgccgtgga ccggcaggac ggcagtcctg accagcgcgt cggcgacagt
      841 gtcggcgtgc cgatcgaagg agatgaatga agctgaagaa cactgctgtg acagcaactc
      901 tgctgggtgc aatgagcgcc gccgccgtcg gcctgggtgc aggcctggcg caggcagagc
      961 cgtttcccgc gccgccgccg tggccagtgc ctgcgccggc tgagccgggc atcggcgtcc
     1021 agggtccgaa cgtcagtaca ccgggtgtgg actttcagac tccgggcgcc gacatcaatg
     1081 gcccgagcgt cagcgcgccg ggcctgggta tcgacggccc ggacgccagc atcggtggtc
     1141 cgagcgtgaa cgtgccaggc gttgacctcc agggcccggc tgccagcgtc gcgccgccag
     1201 cctgggctcc gccgcagccg ccgccgccgc catgggcgcc gactcggccg gtttcgtgga
     1261 atgctgaggc acaggcctgg ggtgtctaca cggacgcggg ctttcagccg gtttaaccaa
     1321 gtgcatggaa tggtcgcctc gccacgcgcg gggcgaccat ttcacggctt cggtggcaat
     1381 gcgtaaccgg tgttttcggc gatcagcgtt ccgctccaca tcagcagtcc tccggtgttg
     1441 cagccgccct cgatgccgtt ggcgtgcacg ctgatcgtcg agtacttgcc gccgttggcc
     1501 ggcttcggaa tgccgtactg cttgccgccg tgaccgggtg acacgcggtc ggcccccaac
     1561 tccctcatgc caccgccggc atctgtgctg atgtgggcga tgatgtccga gcacatgtct
     1621 ggcgacgaga cgaattgcac ggtaaggccg tcgttgttat tcgcaacggt ggtgtagacg
     1681 caggtgtcgc cgctctgatt atcggcgttg caggtgtcca tgctgatctg gaattcctga
     1741 tacgcccatg caggacttgc gagggcaacc gcagccacca cagcaccgaa gacggcggcg
     1801 ccgcgcagcg caggtttggt gagtcgggtg agcctcatat tcagtgtccc ttcggagcgg
     1861 tgtcaattcg ctggaaggcg acgctacggg tatccgatgc ggcgagaaat agggtgtttc
     1921 cctatgtttc agcgcttcta tcggatagct gtggtagctc tgtcagcttg ctgtgaaggc
     1981 acgcccaagg gtgggtggtg cactatttgt gtcaggcatc tttggtgatg gccagcacct
     2041 cggccgcagg cagagcggcg actaccgacg ttgcggcaag cagtttcgag ccacggatgc
     2101 cgcttccgat gatgacctgc tgctggtccg ccacattccg atcgaccagg atcggccagt
     2161 cggcaggcag cccgaccggc gtgatgcccc cggacgccat cccggtcaac gataccgcgg
     2221 tgtccatgga ggcgaacgaa atcttgcgcg cgtcaacgtg tttgcgcacg atgccgttga
     2281 cgtcggcgcg ggtggtcgcc agcaccatac acgccgcgtg ccacacgcgg tcggcgcgct
     2341 tggcctccac gacgacgcag ttcgccgagg catcgagccc gatgccgtag tgctcgcaga
     2401 aggccgcggt atcggcgagg tccgggtcga tctcggtgac ccatagcccg tcggcgccgg
     2461 cctgttcg
//
LOCUS       DASYLX010000494         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3754354,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000494 DASYLX010000000
VERSION     DASYLX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754354"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VGP27_11965"
     CDS             <1..1344
                     /locus_tag="VGP27_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7852054.1"
                     /translation="VPRAQIEEVAREFAAARGAMVVTRTGVSLHLAGTVAEWLGHVLN
                     VITGRLDRPGGRRFEPGYVDAIRMSGMVKASRHRSRLAGREMVAGAHAVSELPDEITT
                     PGRGQIKAMVINCGNPVVSGPDGARLDEALAQLDLLVAIDFVQRESHRHAHWLLPAVH
                     WLERDDLLAFTSNMHDEPYLQFGAKAVDPPPGARQEWRIFTDLAIAMKKPLFKARGLN
                     GFIIGTRRAARLTRMPGLEFGPHWIDRLVVATSRKINGRRVKWRDVLAHPHGWVLGPR
                     EFGHFRDALRTDDKKVHAAPPEFVERARDLLAEPHPAAPDGYPFQLANRRNRHSMNSW
                     LNELPGLRPAGRKNEVVINPEDAAALGVGDGDRARVFSPVGEIELPATVDKRPRPGAI
                     IIDHGWGSRVFDPRGGGAPESFGVNRNLLVDGGPVDPLSQTSALSSSYVGVERVG"
     gene            complement(1463..2119)
                     /locus_tag="VGP27_11970"
     CDS             complement(1463..2119)
                     /locus_tag="VGP27_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEV7852055.1"
                     /translation="MNADELKDRTRRSLAELVNYADELRDLDQALGDGDLGITVTLGA
                     EAVIEAVSALPDSATPAQIARECAKAFANANPSTMAALVAGGLLAGSKIWTEVTEITV
                     ADAASFIRAAGDNIAQRGKTQLGDKTILDAIMPAADALDSASEGTSGLETAIAGAERA
                     VIDTRALQSRRGRASWLQERSIGLQDPGATAFLRFLESWRAVDDTPENGSNPAQTGAQ
                     "
     gene            complement(2124..>2885)
                     /locus_tag="VGP27_11975"
     CDS             complement(2124..>2885)
                     /locus_tag="VGP27_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEV7852056.1"
                     /translation="SSPSSQQCFEAVAAVNGGAGVLLLYGNYGGDVLNFDLATDLAEL
                     EDIAIRTVLGRDDVASQPKERKSDRRGVAGIFFAYKAAGAAAERGDNLEEVAAVVEDV
                     VEHTATMGIGLSPTILPTTGSPSFDLPDGEMEIGIGIHGEPGIHRGELDTADAIADRI
                     VTALVDDLELKSGDRVAALVNGLGATPLEELYILYRRTHQVLSGLGIEIAKSYVGEYA
                     TSLEMAGASISLLALDDERLALLNAPASSPFFRKV"
BASE COUNT          462 a          957 c          982 g          484 t
ORIGIN      
        1 gtcccgcgcg cccagatcga agaggtggcc cgcgagttcg cggccgcgcg cggcgcgatg
       61 gtcgtgaccc gcaccggcgt ctcgttgcat cttgcaggca ccgtcgccga gtggctcggt
      121 catgtgctga atgtgatcac cggccggttg gatcgccccg gcggccgccg gttcgagccc
      181 ggctatgtcg acgcgatccg aatgtcgggc atggtcaagg ccagcaggca ccggagccgc
      241 ctggcaggcc gggagatggt tgcaggcgcg cacgcggtga gcgagttgcc cgacgagata
      301 acgacacccg ggcgcggaca gatcaaggcg atggtcatca actgcggcaa tccggtggtc
      361 tccggacccg atggagccag gctcgacgaa gcgcttgcgc aactggatct gttggtggcc
      421 atcgacttcg tgcagcgcga aagccaccgc cacgcgcact ggttgctgcc cgccgtgcac
      481 tggctggaac gcgacgacct cctcgcgttc accagcaaca tgcatgatga gccgtatctg
      541 cagttcgggg cgaaggccgt cgatcccccg ccgggcgcgc gacaggagtg gcgaatcttc
      601 accgacctcg ccatcgccat gaagaagccg ctgttcaagg cgcgagggct gaacggcttc
      661 atcatcggaa cccggcgggc agcccgcctc acccgaatgc cgggcctgga attcgggccg
      721 cactggatcg accggctggt ggtggccacc agtcgaaaga tcaacggccg cagggtcaaa
      781 tggcgtgacg tcttggcgca cccgcacggc tgggtgttgg gtccgcggga attcgggcac
      841 ttcagggacg cattgcgaac agacgacaag aaggtgcatg ccgcgccacc cgagttcgtc
      901 gagcgcgcac gcgatctgct ggccgaaccg catcccgcgg cgccggacgg ttatccgttc
      961 cagctggcga atcggcgcaa tcggcactcg atgaactcgt ggctcaacga gttgccgggc
     1021 ctgcgtccgg cgggcaggaa aaacgaggtt gtgatcaatc cggaggacgc ggcggcgctg
     1081 ggcgtcggcg acggcgaccg ggcgcgggtg ttctctccgg ttggtgagat cgaactgccc
     1141 gcgacagttg acaagcggcc gcgcccaggc gcgatcatca tcgatcatgg ctggggctca
     1201 agggttttcg atccgcgcgg cggaggtgca cccgagtcgt tcggcgtcaa ccgcaacctg
     1261 ctcgtcgacg gcggccccgt cgacccgctg tcgcagacgt cggcgttgag ctcgtcctac
     1321 gtcggcgtcg aacgcgtcgg ctgacatttg cccctacgcc tccaacgaga cacccgccct
     1381 cccggcgatg ccttcagcgg gttggcgacc attgcgtggc ccatcgtgtc gaggccgacg
     1441 tatccgatcg atgccatatc tctcattggg cgccggtttg ggcagggttg gagccgtttt
     1501 ccggcgtgtc gtcgacagcg cgccacgact cgagaaagcg caagaacgcg gtggcgccgg
     1561 ggtcctgcag gccaatgctg cgttcctgaa gccacgacgc acgaccgcgt cgggactgca
     1621 gagcacgggt gtcgatgaca gcccgttcgg ccccggcgat cgcggtttcc aatcccgacg
     1681 tgccctccga tgcggagtcg agagcgtccg cggcgggcat gatcgcatcg aggatcgtct
     1741 tgtcgccgag ctgggtcttg ccgcgctgcg cgatgttgtc accagcagcc cggatgaagc
     1801 tcgccgcatc cgcgacggtg atttcggtga cctcggtcca gatcttcgag ccggcgagca
     1861 ggccgcctgc gaccagggcg gccatcgtcg acgggttggc gttcgcgaac gcctttgcgc
     1921 actcgcgcgc aatctgcgcg ggagtggcgg agtccggcag cgccgagacg gcctcgatga
     1981 ccgcctccgc gccaagagtg accgtgattc cgaggtcgcc gtcgcccagc gcctggtcga
     2041 gatcgcgcag ctcgtcggcg tagttcacca gttccgcgag gctccgccgg gtgcggtcct
     2101 tcagttcatc ggcgttcacg cgctcagact ttccggaaga acggagacga tgccggcgcg
     2161 ttgagcagag ccagtcgctc gtcgtcgagt gcgagcagag agatcgacgc cccggccatt
     2221 tccagactgg tggcgtactc gccgacatag cttttggcga tctcgatccc aaggccgctg
     2281 agcacctggt gggtccggcg gtacagaatg tagagctcct cgaggggcgt ggcaccaagc
     2341 ccgttgacca gcgcggccac cctgtcgccg gacttcagct ccagatcgtc gaccaaagcg
     2401 gtgacgatcc ggtcggcgat ggcgtccgcg gtgtccaatt ccccgcggtg gatgcccggc
     2461 tcgccgtgga tcccgatgcc gatctccatc tcaccgtccg gcagatcgaa gctcggagaa
     2521 ccggtcgtcg gcaggatcgt tggggacaga ccgatgccca tcgtcgcggt gtgctccacc
     2581 acgtcctcga ccacggccgc gacctcctcc agattgtcac cccgctcggc ggcggccccg
     2641 gcggccttgt aggcgaagaa gattcccgcg acgccgcggc ggtcgctctt gcgctctttt
     2701 ggttggctgg cgacatcgtc tcggccgagc acggtgcgga tcgcaatgtc ctccagctcg
     2761 gccaggtcgg tggcgaggtc gaagttgagg acgtcgccgc cgtagttgcc gtacagcaac
     2821 agcaccccgg cgcctccgtt caccgccgcg accgcctcga aacactgctg cgagctcggt
     2881 gagct
//
LOCUS       DASYLX010000495         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1998261,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000495 DASYLX010000000
VERSION     DASYLX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998261"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="lepB"
                     /locus_tag="VGP27_11980"
     CDS             <1..774
                     /gene="lepB"
                     /locus_tag="VGP27_11980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV7852057.1"
                     /translation="VHEQAAEAADKSKRKRGALREAVILVVIAVGLYYLMLTFIARPY
                     LIPSESMEPTLHGCSGCVGDRIMVDKLTYRFSKPEPGDVVVFKGPPNWNAGYKSIRSD
                     NPAVRAVQNALSFVGFVPPDENDLVKRVIATGGQTVECRQNTGLTVDGKPLKEPYLDL
                     KTMNVPDPDKYRCLGFEFGPVKVPDGRVWVMGDNRTHSADSRVHCADNPADFQSGIKC
                     TGDPNAGTVPLGNVIGKARFIAWPPSRWGGVSSVDPQTQ"
     gene            787..1509
                     /locus_tag="VGP27_11985"
     CDS             787..1509
                     /locus_tag="VGP27_11985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV7852058.1"
                     /translation="MPATWPPRAVIRKSSGLRTLESALYRSGLGPVAGVDEVGRGACA
                     GPLVVAACMLGPNRLDSLAALDDSKKLTEKERERLFPLIRRYALAYHVVFIPSVEVDR
                     RGVHVANIEGMRRAIAGLPVRPGYVLSDGFRVPGLTAPSLPVVGGDAAAACIAAASVL
                     AKVSRDRLMVKMEAEHPGYGFADHKGYSTPAHGAALARLGPCSQHRYSFINVRRVATP
                     TGIKVVTELAGDCVPAQHGEVG"
     gene            1551..1856
                     /locus_tag="VGP27_11990"
     CDS             1551..1856
                     /locus_tag="VGP27_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEV7852059.1"
                     /translation="MSAEDLEKYETEMELSLYREYKDIVGQFSYVVETERRFYLANSV
                     EMVPRNADGEVYFELRLADAWVWDMYRPARFVKQVRVITFKDVNIEEVEKPELRLPE"
     gene            complement(1861..>2084)
                     /locus_tag="VGP27_11995"
     CDS             complement(1861..>2084)
                     /locus_tag="VGP27_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7852060.1"
                     /translation="GIGAAPKLQKVVDEKDIDADIKVYPGAGHSFANKLPAQPLIRIA
                     GFGYHEAAESDAWSRVFAFFGEHLHETAG"
BASE COUNT          371 a          648 c          721 g          344 t
ORIGIN      
        1 gtccacgaac aggccgccga ggccgccgac aagtcgaagc gcaagcgtgg ggcgctgcgc
       61 gaggccgtca ttctggtggt gatcgcggtc ggcttgtact acctgatgct gaccttcatc
      121 gcccgtccct atctcatccc gtcggagtcg atggagccca cgttgcacgg ttgctccggc
      181 tgtgtcgggg accgcatcat ggtcgacaag ctgacctatc ggttctccaa gcccgagccg
      241 ggcgatgtgg tggtgttcaa gggcccgccg aactggaacg ccgggtacaa gtcgatccgc
      301 tcggacaacc ccgcggtgcg agcggtgcag aatgcgctct cgttcgtcgg gttcgtcccg
      361 ccggacgaga acgatctggt caagcgggtg atcgcgaccg gaggccagac cgtcgaatgc
      421 cgtcagaaca cggggctgac cgtcgacgga aagccgctca aggagcccta cctcgacctg
      481 aagacgatga acgtgcccga ccccgacaag taccgctgtc tgggcttcga attcgggccg
      541 gtgaaggtgc ccgacggccg ggtgtgggtg atgggcgaca atcgcaccca ttccgccgat
      601 tcgcgcgtgc actgcgctga caacccagcc gatttccaga gtgggatcaa gtgcaccggc
      661 gaccccaatg cgggcaccgt tccattgggg aacgtcatcg gtaaggcacg gttcatcgcc
      721 tggccgccat cgcgatgggg tggggttagc tcggtggatc cgcagactca gtaggagggt
      781 gcgagcttgc cggcgacatg gccgccacga gcggtgatcc gcaagtcatc cgggcttcgc
      841 acgctggagt cggcgctgta ccgctccgga ctcggcccgg tggccggtgt ggacgaggtc
      901 ggccgcggcg cctgcgccgg accgctcgtc gtcgcggcgt gcatgctcgg gccgaaccgg
      961 ctggacagcc tcgccgcgct cgacgactcc aagaaactca ccgagaagga acgcgagcgg
     1021 ctcttccctc tgatccggcg gtatgcgctg gcgtatcacg tggtgttcat cccgtccgtc
     1081 gaggtggacc gccgaggagt gcacgtggcc aacatcgagg gaatgcgcag ggcgatcgca
     1141 ggactgccgg tgcgaccggg atacgtgctc agcgacggct tccgggtgcc ggggctgacc
     1201 gcgccgtcac taccggtggt cggcggcgac gccgctgcgg cctgtatcgc cgccgccagc
     1261 gtgttggcca aggtcagccg tgaccgcctg atggtgaaga tggaggccga acaccctggc
     1321 tacggcttcg cagaccacaa gggctacagc acgcccgcgc acggggcggc gctggcccgg
     1381 ctcggcccgt gcagccagca ccgatactcg ttcatcaacg tccgccgggt ggccacgccg
     1441 accggcatca aggtcgttac ggagctcgcg ggcgactgcg taccggcgca gcacggcgaa
     1501 gtcgggtagg ggacaatgtt tctagaccaa tacgaaggac tgttgaagcg atgagtgccg
     1561 aagatctcga gaagtacgaa accgagatgg agctctcgct gtaccgcgaa tacaaggaca
     1621 tcgtcgggca gttcagctac gtggtggaaa ccgagcgacg gttctacctg gcaaacagtg
     1681 tcgaaatggt gccccgcaac gccgacggtg aggtgtactt cgagctgcgg ctggccgacg
     1741 cctgggtgtg ggacatgtac cggccggccc ggttcgtcaa gcaggtacgg gtgatcacat
     1801 tcaaggacgt gaacatcgaa gaggtggaga agcccgaact gcggctgcct gaataagcgg
     1861 tcagccggcg gtctcgtgca ggtgctcgcc aaagaacgcg aagacccgcg accaggcgtc
     1921 ggattccgcc gcctcgtggt agccgaatcc ggcgatgcgg atcagcggct gagccggaag
     1981 tttgttggcg aagctgtgcc cggcgcccgg gtagaccttg atgtcggcgt cgatgtcctt
     2041 ctcgtcgacg accttctgca gcttcggcgc cgcgccgata ccga
//
LOCUS       DASYLX010000496         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1339235,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000496 DASYLX010000000
VERSION     DASYLX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339235"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VGP27_12000"
     CDS             <1..1102
                     /locus_tag="VGP27_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEV7852061.1"
                     /translation="LKTRLAATRWPEQLPGTPWQRGVPVDYLRRLAGYWADGFDWRAQ
                     EADLNSYPQFRTRIDGQTVHFLHVQSPELSARPLILLHGWPGSVVEFLDIIGPLSNPR
                     AHGLDPTQAFHLVIPSLVGFGFSTPLSGAGWTATRIAQACTELMAGLGYPRYGVQGGD
                     YGSFIGPVMGSLAPDRVFGVHVNATAFGFMPGKDLTDEDFAALTDLERDKVALGRAWA
                     AEKTGYFKVQGTRPQTLTYGLTDSPVGQLAWIVEKFREWTNAEKELPEDSVPRDRLLA
                     NVSVYWFTGTAGSSANLYYETTHNRAMPEPSGVPTGVAVFAEDAAIRRYGEKWHNITH
                     WSDFDTGGHFAAMETPELLVNDVRAFFSTVTQ"
     gene            1199..2323
                     /locus_tag="VGP27_12005"
     CDS             1199..2323
                     /locus_tag="VGP27_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7852062.1"
                     /translation="MPSGMAGAQQRLDSAAVDDPQIELIGVRKEFSDTAHARSKIGRG
                     HKVVAVEGADLAVGDGELFAILGPSGSGKTTVLRMVAGFEQPTAGTIRLGGTDVTALP
                     ARRRDVNTVFQEYALFPHMTVAQNVEYGLKVKGVPGDKRRKRTSDALDMVRLSGEASR
                     KPAQLSGGQRQRVALARALVGRPRVLLLDEPLGALDLKLREQMQIELKEIQREVGITF
                     VIVTHDQDEALTLCDRLAVFNNGRIEQIGEARDVYENPANRFVADFVGTSNLLDGSAA
                     EALVGRPGTYVIRPERIAVFARGTDPVSGVRAVDATVLEVIYAGPTTKIAAVTEQGVT
                     LTATVLTASTWLPPDLQHGTRVTLAWPEKALHQLSNEESP"
     gene            2320..>2930
                     /locus_tag="VGP27_12010"
     CDS             2320..>2930
                     /locus_tag="VGP27_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7852063.1"
                     /translation="MKAVRIGLVLGACAALIVACSGSEESSGTGESPPKLEPLSSVGN
                     GEGQLNIIAWAGYAEDGSNDKTVDWVTPFEKETGCQVNVKLGNTSDEMVQLMRTGQYD
                     GVSASGDATLRLIYAGDVAPVNTDLVPNYATISDFLKDKSWNSVDGQMYGVPHGWGAN
                     LLMYNLDVVRDAPNSWSAVFDGADKYKGKVTAYDSPIYIADAAL"
BASE COUNT          524 a          994 c          957 g          455 t
ORIGIN      
        1 cctcaagacc cggctggccg cgacgcgctg gcccgagcag ttgccgggca caccgtggca
       61 gcgaggcgtg cccgtcgatt acctgaggcg gctggccggc tactgggccg acggcttcga
      121 ctggcgcgcc caggaggccg atctgaactc ctacccgcag ttccgcaccc ggatcgacgg
      181 ccagacggtg cacttcctgc acgtccagtc gccggaactg agcgcgcgac cgctgatcct
      241 gctgcacggc tggcccggct cggtcgtgga gttcctcgac atcatcggcc cgctgagcaa
      301 tccgcgcgcc cacggcctcg accccacgca ggcgttccac ctcgtcatcc cgtcgctcgt
      361 cggcttcggc ttctcgaccc cgctgtcggg ggccggctgg accgcaaccc gcatcgcgca
      421 ggcctgcacc gagctgatgg ccgggctggg ctacccgcgc tacggcgtgc agggcggcga
      481 ctacggctcg ttcatcgggc ccgtcatggg cagcctcgcc ccagaccgag tgttcggtgt
      541 gcacgtcaat gcgaccgcat tcggattcat gcccggaaaa gacctgaccg acgaggactt
      601 cgcggcgctg accgacctcg aacgcgacaa ggtggcgctg ggcagggcat gggccgcgga
      661 gaagaccggc tacttcaaag ttcagggcac ccggccgcag acgctgacct acgggctgac
      721 agattcgccc gtcggacagc ttgcctggat cgtcgagaag ttcagggaat ggacgaacgc
      781 cgagaaggag ctgcccgagg actcggtgcc gcgcgatcgt ctgctggcca acgtttcggt
      841 gtactggttc accggcactg cggggtcgtc ggccaacctc tactacgaga ccacccacaa
      901 tcgggcaatg ccagagccgt ccggcgtgcc caccggcgtc gccgtgttcg cagaggatgc
      961 ggccatccgc cgatacggcg agaaatggca caacatcacc cactggagcg acttcgacac
     1021 cggcggacac ttcgccgcga tggagacccc cgagttgctc gtcaacgacg tccgggcatt
     1081 cttttcgacg gtcactcaat aagcggattc ggcgcggaaa cgtgcgctga gcgccggtga
     1141 gcgcgccgaa atcgctgatg acgctgcggg tacaccgcgg aattcgtagg atctgccaat
     1201 gccctcgggg atggccggcg cccagcagcg cttggattcc gctgccgttg acgatcctca
     1261 gatcgagttg atcggcgtcc gcaaggaatt cagcgacacc gcgcatgcga ggagcaaaat
     1321 cggccggggc cacaaggtgg tcgccgtgga gggcgctgac ctcgctgtcg gcgacggcga
     1381 gctgttcgcg atccttggcc cgtccgggtc cgggaagacc accgtgctgc gcatggtggc
     1441 cggtttcgaa cagccgacgg cgggcacgat ccgcctcggc ggcaccgacg tcaccgcgct
     1501 gcccgcgcgg cgccgcgacg tcaacaccgt cttccaggaa tatgccctgt tcccccacat
     1561 gaccgtcgcc cagaacgtgg agtacgggtt gaaggtcaag ggcgtgccgg gcgacaaacg
     1621 ccgcaaacgg acctccgacg cactcgacat ggtccggttg agcggcgaag cgtcacgaaa
     1681 gcccgcgcag ctgtcgggcg gtcagcgtca gcgggtcgcg ctggcgcgcg cgctcgtcgg
     1741 gcggccccgg gtgctgctcc tcgacgagcc cctcggcgca ctggatctca agctccgcga
     1801 acagatgcag atcgagctga aggagatcca gcgcgaggtc ggcatcacgt tcgtcatcgt
     1861 cacccacgat caggacgagg cgctgaccct ctgtgaccgg cttgcggtgt tcaacaacgg
     1921 ccgcatcgag cagatcggcg aggcgcgcga cgtctacgag aaccctgcca accggttcgt
     1981 cgccgacttc gtcggaacct ccaaccttct cgacggatcc gccgccgaag ctcttgtcgg
     2041 ccggccgggc acctacgtga ttcgaccgga acgcatcgcg gtgttcgcac gcggaaccga
     2101 tccggtgtcc ggcgttcggg cggtggacgc gacggtgctg gaggtcatct acgccggacc
     2161 caccacgaag atcgccgcgg tcaccgaaca gggcgtcacg ttgaccgcga ccgtccttac
     2221 ggccagcacc tggctacccc cagatttaca gcacggcacg cgagtcacct tggcgtggcc
     2281 cgaaaaggcc cttcatcaac tgtcaaacga ggagtcgcca tgaaagcagt tcggatcggt
     2341 ctcgtcctcg gcgcctgcgc cgccctgatc gtcgcgtgct ctggctccga ggagtccagc
     2401 ggaacgggcg agtccccgcc gaaattggag ccgctgagct cggtcggcaa cggcgaaggc
     2461 cagctgaaca tcatcgcgtg ggcgggctac gcggaagacg gctccaacga caagaccgtc
     2521 gactgggtga ccccattcga gaaggagacc ggctgtcagg tgaacgtcaa gctgggcaac
     2581 acatctgacg agatggtcca gctgatgcgc accggtcagt acgacggggt gtcggcatcc
     2641 ggtgacgcga cgttgcggct gatctacgca ggcgacgtcg caccggtgaa cacggacctg
     2701 gtgccgaact acgcgacgat ctccgacttc ctcaaggaca agtcgtggaa ctccgtcgac
     2761 ggccagatgt acggcgtgcc ccacggctgg ggcgccaacc tgttgatgta caacctcgac
     2821 gtcgtgcgtg atgcaccgaa ctcatggagc gccgtgttcg acggcgccga caagtacaag
     2881 ggcaaggtga ccgcctacga ctcccccatc tacatcgccg acgccgcgct
//
LOCUS       DASYLX010000497         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_243296,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000497 DASYLX010000000
VERSION     DASYLX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243296"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VGP27_12015"
     CDS             <1..1593
                     /locus_tag="VGP27_12015"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEV7852064.1"
                     /translation="SGIDTIFTLSGGHLFSIYDGCRAEGVRLIDTRHEQTAAFAAEGW
                     SKVTRVPGVAALTAGPGVTNGMSAMGAAQQNQSPLLVLGGRAPALRWGQGSLQEIDHV
                     PFVAPLTRFAATAQSADAVGGLIDDALRAAMGAASAAGSPMGVAFVDFPMDHVFGEAH
                     DAGDPGALAGPPERAQADGEALAIAAGLLADAQRPVIMAGTNVWWGHAEGALLALAEA
                     HRIPVLMNGMARGTVPADHELAFSRARSKALKEADVALVIGVPMDFRLGFGGVFGEHT
                     RLIVADRAVPERPHPREVAAELYGDLTATLSTLSGGRSGDRETWISDLRVVESAGRAA
                     EEAELTDDRSPLHPMRVYAELKPLLDRDAIVVIDAGDFGSYAGRVIDSYVPGAWLDSG
                     PFGCLGSGPGYALAAKLARPDRQVVLLQGDGAFGFSGMEWDTLVRHGVHVVSVIGNNG
                     IWALEKHPMEALYGYSVVAELRPGTRYDEVVTALGGHGELVSAPAQLRPALERAFASG
                     VPAVVNTLTDPQVAYPRRSNLA"
     gene            1753..>2544
                     /locus_tag="VGP27_12020"
     CDS             1753..>2544
                     /locus_tag="VGP27_12020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7852065.1"
                     /translation="MIVRTHASVDEFRAVAEPLYRRDPVRNTIELTLLRADRFGDDAL
                     LLTVWDEEVAVGAALQTPPYPMACNGIPVAAHHVVASELARTRRELNGVRGSRDEALA
                     FADTWRDVTGRDGIVTTGERLYRLATLRPPAHVSGLRRDATRDDNAVLVDWVQRFFVE
                     TFSHRRDVAAGRRFVDNADAVGDRFVVWDDGGTPVSMAMLRAPSSGVSRIGPVFTPRS
                     ERGRGYGSAVTAAAADLARRGGVEEVVLFADLANQTSNGIYQRIGF"
BASE COUNT          357 a          855 c          925 g          407 t
ORIGIN      
        1 agcggtatcg acacgatatt cacgctctcc ggtgggcatc tgttctccat ctacgacggg
       61 tgccgcgccg agggtgtccg gctgatcgac accagacacg agcagaccgc ggcgttcgcg
      121 gccgaaggct ggtcgaaggt cacccgggtg cccggggtcg cggcgctgac cgcgggtccc
      181 ggcgtgacga acgggatgag tgcgatgggc gccgcccagc agaaccagtc gccgctgctg
      241 gtgctcggcg gccgggcgcc cgcgctgcgc tggggtcagg gctcacttca ggaaatcgat
      301 cacgtgccgt tcgtggcgcc gctgacccgg ttcgcggcga ccgcacagtc ggccgacgcg
      361 gtcggcgggc tcatcgacga cgcgctgcgc gcggccatgg gcgccgcgag cgcggcaggt
      421 tctccgatgg gggtcgcgtt cgtcgacttc ccgatggatc acgtgttcgg cgaagcccac
      481 gacgcgggag accccggtgc gcttgccggg ccaccggaac gggcgcaggc cgacggggag
      541 gcgctcgcga tcgcggccgg tctgctcgcc gacgcgcaac ggccggtgat catggccggc
      601 accaacgtgt ggtgggggca cgccgaaggc gccctgctgg ctctggccga ggcgcaccgc
      661 attccggtgc tgatgaacgg catggcgcgc ggaaccgtgc cggccgacca cgagctggcg
      721 ttctcgcgcg cgcggtcaaa ggcgttgaag gaggccgacg ttgcgctggt gatcggcgtt
      781 ccgatggact tccgcctcgg gttcggcggc gtgttcggcg agcacacccg actgatcgtc
      841 gccgaccgcg cggtgcccga gcggccgcat ccgcgcgagg tcgccgccga gttgtacggc
      901 gacctgacgg cgacgttgtc gacgctgtcc ggtgggcgat cgggcgaccg tgaaacgtgg
      961 atcagcgatc tgcgggtcgt ggagtctgcg ggccgtgccg ccgaggaggc cgaactgacc
     1021 gacgaccgca gtccgctgca cccgatgagg gtgtacgccg agctgaagcc gctgttggac
     1081 cgcgacgcga tcgtggtgat cgacgcaggc gacttcggct cctatgccgg ccgggtcatc
     1141 gacagctatg tgcccggcgc gtggttggac agcggaccgt tcggctgcct cggctcgggt
     1201 cccggctacg cgttggccgc caaactggcc cgacctgacc ggcaggtggt cctgctgcag
     1261 ggtgacggcg cgttcggctt cagcgggatg gagtgggata cgttggtgcg ccacggggtt
     1321 catgtcgtct cggtgatcgg caacaacgga atctgggcgc tggagaagca cccgatggag
     1381 gccttgtacg gctattcggt ggtggccgag ctgcggccgg ggacccgcta cgacgaggtg
     1441 gtcaccgccc tcggtgggca cggggagctg gtgtccgctc ccgcccagct tcggcctgcg
     1501 ctggagcggg cgttcgcgtc gggtgtgccc gccgtggtga acacgctcac cgaccctcag
     1561 gtcgcatatc cgcgtcgatc gaacctggcc tgacttcccc ccgcgacgtg gggagttggt
     1621 gccgtgccac ctccccttct gcgcgagcgt gcgtgtctgc gcacgacacg ccgggcagat
     1681 atgtacaact gcgcacggtc gcggtgcaaa attcactgga cgggccgagc aatcggtgct
     1741 catctggtgt gcatgatcgt gcgcacacac gcgtcggtgg acgagtttcg cgccgtcgcc
     1801 gaaccgctgt accgccgcga ccccgtcaga aacaccatcg agctgaccct gctgcgagcg
     1861 gacaggttcg gcgacgacgc gcttctgctc actgtgtggg acgaggaggt cgctgtcggc
     1921 gcagcactgc agaccccgcc gtacccgatg gcatgcaatg gaattcccgt cgcagcccac
     1981 cacgtcgtcg cgtcagaact ggcgcgtacc cgccgcgaac tcaacggtgt acgaggctcc
     2041 cgcgacgagg cactggcctt cgccgacacc tggcgcgacg tcactggacg cgacggcatc
     2101 gtgacgaccg gggaacggct ctaccgcctg gcgaccttgc ggccgccagc acacgtctcc
     2161 ggccttcgcc gcgacgccac ccgtgacgac aatgccgtgc tcgtcgactg ggtccagcgc
     2221 ttcttcgtcg agacgttcag tcaccggcgc gacgtcgcag cgggccggcg gttcgtcgac
     2281 aacgccgacg ccgtcggcga ccgcttcgtg gtctgggacg acggcggcac gccggtgagc
     2341 atggcgatgc tgcgcgcgcc gtcgtccggt gtttcccgta tcggtcccgt cttcacgccg
     2401 cgcagcgagc gcggccgcgg ctacgggtcg gccgtcaccg cggcggcggc cgatctggcg
     2461 cgtcgtggcg gcgtcgagga ggtggtgctg ttcgccgacc tggccaacca aacgtccaac
     2521 ggcatctatc agcgcatcgg attc
//
LOCUS       DASYLX010000498         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2876605,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000498 DASYLX010000000
VERSION     DASYLX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876605"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..75)
                     /locus_tag="VGP27_12025"
     CDS             complement(<1..75)
                     /locus_tag="VGP27_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7852066.1"
                     /translation="MAEPLLQAQINIDAPVAKVWALISD"
     gene            complement(106..369)
                     /locus_tag="VGP27_12030"
     CDS             complement(106..369)
                     /locus_tag="VGP27_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HEV7852067.1"
                     /translation="MSAEPPEAADPELPVLPAILLEPWPVIIVVAVMWLVVVVLAFTV
                     AGLHEWRPVAVAGLGVGALGTSIFLWQRSAVRRGSRGAQSGLT"
     gene            459..890
                     /locus_tag="VGP27_12035"
     CDS             459..890
                     /locus_tag="VGP27_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7852068.1"
                     /translation="MNNSETRLASDLSLAVMRLARHLRFRRPGSSVSLSQLSALSTLA
                     KEGAMTPGALALRERVRPPSMTRVIASLAELGFVARSAHPADGRQVLVSVSPSGTELI
                     EAERKASQEWLQERLAQFDADQRKALLVAADLMSALVDESA"
     gene            919..>1684
                     /locus_tag="VGP27_12040"
     CDS             919..>1684
                     /locus_tag="VGP27_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV7852069.1"
                     /translation="MSPVVDIDDPADPRLDDFRHLNSVDRRPDLPSGKGLVIAEGVLV
                     VQRMLASRFRPRAMLGTDRRLCELAADLDVTDAPYYRATAEVMADVVGFHLNRGVLAA
                     ASRSDELTVGQVLDGARTVVVLEGVNDHENLGSIFRNAAGLGIDAVVFGTGCADPLYR
                     RAVRVSMGHALLVPYAWATAWPTDLSILRDNDFRLLAMTPAASEATLSEAMATVADDR
                     VAILVGAEGPGLTETAMRASDVRVRIPMSRGTDSLNV"
BASE COUNT          288 a          530 c          550 g          316 t
ORIGIN      
        1 atcggagatc aacgcccaca cctttgctac tggagcgtca atattgattt gcgcctgcaa
       61 tagcggttcg gccatctcat cctccttgtc gctgaatctg gaaattcagg taagtccgct
      121 ctgtgcgcct cgtgaaccgc gacgcacagc actccgttgc caaagaaaaa ttgaagtgcc
      181 aagagcaccg actcccaacc cggcgacggc gacggggcgc cactcatgta gcccggcgac
      241 ggtgaaggcg aggacaacaa cgaccaacca catcacggcg acgacgatga tgaccggcca
      301 cggctccagt agaatcgcag gcagtacagg cagttccgga tcggcggcct cggggggttc
      361 ggcgctcatc ggttcccaac ctagtcggaa gctcgttcgg ccggaagcct gaggtgtttt
      421 gctgcgcatg aaagaactgc ttcgctattg tttgcattgt gaacaattcc gaaactcggt
      481 tggcgagtga cttgtcgctg gcggttatgc gtttggcgcg tcatttacgg tttcgccgcc
      541 cgggttcgtc ggtctcccta tcgcagttgt cggcactatc gacgcttgct aaagaaggcg
      601 caatgacgcc cggtgcattg gcgcttcgtg aacgcgttcg gccgccctcg atgacacgcg
      661 taatcgcgtc gctggccgaa ctcggattcg tggcgagaag tgctcatccc gccgacggtc
      721 gccaggtctt ggtgtccgtt tcgccgtcgg ggacggaatt gatcgaagca gagcgaaaag
      781 ccagccagga atggctgcag gagcgcctcg cgcagttcga tgccgaccag cgcaaggcgc
      841 tgctcgtagc tgccgacctg atgtcggcgc tcgtcgacga atcggcgtag gcccggtcgc
      901 acgcgtagct tgcgggagat gagtcccgta gtcgacatcg acgatcccgc cgatccgcgc
      961 ctcgacgact tccgtcatct caacagcgtc gaccgcaggc ccgacctgcc gtccggcaag
     1021 ggcctggtga tcgccgaggg cgtcctggtg gtgcagcgga tgctggcgtc ccggttccgg
     1081 ccacgcgcaa tgttgggcac cgatcgccgg ctgtgcgaat tggccgccga tctcgacgtg
     1141 accgacgcgc cctactaccg ggcgacggct gaggtgatgg ccgacgtggt cgggttccat
     1201 ctcaaccggg gggtgctcgc agccgcgtcg cggtccgatg agttgacggt cggtcaggtg
     1261 ctcgacggcg cgcgaacggt cgtggtgctc gaaggcgtca acgaccacga gaacctcggc
     1321 tcgatcttcc gaaacgcggc cgggctcggc atcgacgcgg tggttttcgg caccggctgc
     1381 gccgacccgc tgtaccgccg cgcggtgcgg gtgtcgatgg gccatgccct gctggtgccc
     1441 tatgcctggg ccacggcctg gccgactgac ctgtccatat tgcgtgacaa cgactttcgc
     1501 ttgttggcga tgacgcccgc cgcgagcgaa gccacgctgt cggaggcgat ggcaacggtc
     1561 gccgacgacc gcgtcgcgat actcgtcggc gccgaaggcc ccggtctcac cgaaacggcg
     1621 atgcgtgcca gcgacgtccg ggttcggatt ccgatgtccc gtggcaccga ctcgctgaat
     1681 gtgg
//
LOCUS       DASYLX010000499         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1779253,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000499 DASYLX010000000
VERSION     DASYLX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779253"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGP27_12045"
     CDS             <1..265
                     /locus_tag="VGP27_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852070.1"
                     /translation="RVTPSVIVALSGVRTRYDDLMATAANGPGATLGQRLYIARGRAK
                     LTTKEAANGVGLRKDLIEAVESEEPVLEDEASRIKDLIAALGG"
     gene            390..1064
                     /locus_tag="VGP27_12050"
     CDS             390..1064
                     /locus_tag="VGP27_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7852071.1"
                     /translation="MERNLANAALFRGIEPEVVSGLTAQLETADFAAGETIFARGDLS
                     AALYLLVDGKVKLGRCADDGRENLLTVLAPPDMFGELSLLDPGPQPWTATAVTDARTV
                     LVNRAQLDAWMIDHPAMGDRLLRLMARRLRHTTDTLADLAFIDAPARVAKQLLGLAQR
                     FGTPENGSMRVTHDLTQEELAHLVGATRETVNKALSDFRNRGWIRVDGKSVLISDSER
                     LAHRAH"
     gene            complement(1074..2183)
                     /locus_tag="VGP27_12055"
     CDS             complement(1074..2183)
                     /locus_tag="VGP27_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852072.1"
                     /translation="MGVSALRVAACAGVLVGLLVLGTGSAAVAIAAPGDDAPSQSDDK
                     SSNAPANEGAGQGGVNHAAAGEEHQSATEPNVATSGDAGEPAPETAATTERAATSDGA
                     ESGSGTEGAVTVESGGGSGYGEIAPAAVAETGGEGDSVDNTAAKVEPEPVPKVTSFAS
                     YPFPYYLLIRRDGGAWWNANQIISRFLGVTKPQPEPEPTPSPAFRGGAPEPEPVLDAS
                     GGVTGGGGSDYQATGFGAAPVISAPIVAPPAPPPASARFPSFPAAATTAAGVGSAAAR
                     TGYAESGSTAEGVPPAGQLGQPPAGTVKAMSGQTPPRGYTDYLRSPTLGQLAGAALPG
                     VAGILLMTFGGGVIGYRQAEAGRMIRAGGAARYLP"
     gene            2315..2827
                     /locus_tag="VGP27_12060"
                     /pseudo
     CDS             2315..2827
                     /locus_tag="VGP27_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
BASE COUNT          421 a          986 c         1002 g          452 t
ORIGIN      
        1 ccgggtgacc ccgtcggtca tcgtggcgct cagtggtgtc cgcacccgct acgacgatct
       61 gatggccacc gcggccaacg ggccgggtgc cacgctgggt cagcgcctct acatcgcccg
      121 cggccgggcg aagctcacca ccaaggaagc ggccaacggt gtcggcctgc gcaaagactt
      181 gatcgaggcc gtcgaatccg aggagccggt gctcgaggac gaggcttcgc gaatcaagga
      241 tctcatcgcc gccctcggcg gctgacgccg aagcactcgg ctgttttcgc gcgcggcgtt
      301 tgcaccgggt gtcagcgggt accggcacac cttaagtcgg tgggccaaaa caggttggtg
      361 ccacaatcgc tctatcgcat tgaggcgcca tggaacgcaa ccttgcaaac gctgcattgt
      421 tccgcggaat cgaaccggag gtcgtctccg ggctcaccgc tcaactcgaa acggccgact
      481 tcgctgccgg cgagaccatc ttcgcaaggg gcgatctcag tgccgcgctc taccttctcg
      541 tcgacgggaa ggtgaagctg ggccgctgcg ccgacgacgg ccgggagaac ctcctcaccg
      601 tgctggcgcc gccggacatg ttcggcgagc tgtccctcct cgatccggga ccgcagccgt
      661 ggaccgccac cgcggtcacc gacgcccgta cggtgctggt caatcgtgcg cagctggacg
      721 cctggatgat cgatcatccc gcgatggggg accgcctgct gaggctgatg gcccggcggt
      781 tacgccacac caccgacacc ctcgccgatc tggcgttcat cgacgcgccc gcacgggtcg
      841 ccaaacaact gctcgggctg gcgcagcgat tcggcacccc ggagaacggg tcgatgcgag
      901 tgacgcacga tctgacccag gaagagctgg ctcacctggt cggtgcgacc agggagaccg
      961 tgaacaaggc gctgtcggat ttccgcaacc gcgggtggat ccgcgtcgac ggcaagagcg
     1021 tgctcatctc ggattccgaa cgcctggctc accgggcgca ctgacgagcg cggctagggc
     1081 aggtagcggg cggcgccgcc ggcgcggatc atccggcccg cctcggcctg ccgatagccg
     1141 atgacgccgc cgccgaaggt catcagcagg atgcccgcga cgcccggcag cgcggcgcct
     1201 gccagctgcc cgagagtggg gctgcgcagg tagtcggtgt atccacgtgg cggggtctga
     1261 ccggacatcg ccttgacggt gcccgcgggc ggctgtccca gttggcctgc aggcgggacg
     1321 ccctcggcgg tcgagccgga ttcggcgtac cccgtgcgcg cggcggccga accgacacct
     1381 gccgcggtcg tcgcagcggc agggaacgac ggaaagcggg ccgaggcggg gggcggtgcc
     1441 ggcggcgcga cgatgggcgc cgaaatcacc ggcgctgccc cgaacccggt cgcttgatag
     1501 tcgctgccgc caccgcccgt cacaccgccg gacgcgtcga ggaccggctc gggctccggc
     1561 gctccgccgc ggaatgcagg agacggcgtc ggctcgggtt ccggctgcgg cttcgtgacg
     1621 ccgaggaaac gcgagatgat ctggttcgcg ttccaccagg ccccgccgtc gcgccggatc
     1681 agaaggtaat acgggaaggg gtaggaggcg aagctggtca ccttcggcac cggttccggc
     1741 tccaccttgg cggcggtgtt gtcgacggaa tcgccttcac cgcccgtttc ggctacggcc
     1801 gcgggcgcga tctctccgta accggagccg ccgccggatt cgactgtcac ggcaccctca
     1861 gtacctgagc cgctttccgc cccgtcggag gtggcggcac gctcggtggt ggccgctgtc
     1921 tcgggtgcgg gttcgcccgc atcgccggac gtcgcgacgt tgggttccgt ggcgctctga
     1981 tgttcttcgc cggcggccgc gtggttcacg ccgccctgac cggcgccttc gttagcgggg
     2041 gcgttgctcg acttgtcgtc gctctgactg ggcgcgtcat cgcccggggc tgcgatggcc
     2101 acggcggctg aaccggtgcc gagcacgagg agaccgacca gcacgccggc acaggccgcg
     2161 acgcgcagag ccgacacccc cattagccct catttctcga tgaacatctc gctgactagg
     2221 cgtttccggt gagggaattc tcgcatactt ctcgcgaccg atcgggcgga acgtttgctt
     2281 gtcggcattc atgcgacgac ggtgacctgc gacgttggag tatcgtgccg acgtggcatc
     2341 ccgatatcgg ccgtcggccg ccgagggggc gcgcaccatt gccgcaggca ccaacgcggg
     2401 cacgctggcg acgctctcct cggccggtga gccgtgggcc tcgttcgtca cctacgggtt
     2461 gctggatgga gcgccggtgc tgtgcgtctc gaacctcgcc gagcacggcc gcaaccttct
     2521 cgccgatcac cgagccagca cctcgattgt ggcgcccggc ccggacccgg acccgcagtc
     2581 gtggggacgg gtgacactcg ccggttgcgt cgaacgaccg gtcggcgatg aactcgccgc
     2641 cgcgagggac gcgcatctgg ccgcggttcc gggtgcgcag aactacatcg acttcggcga
     2701 cttcgccctg tgggtgctgc gggtgcagcg ggtgcgctgg tatggcggct acggccggat
     2761 ggacgtggcc acgggcgatg cctactcggc ggccgcaccc cacccgatct cgccgcgctc
     2821 ggcggggacc gctacggacc cgatttgatt tacgctcggc c
//
LOCUS       DASYLX010000500         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2876697,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000500 DASYLX010000000
VERSION     DASYLX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876697"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGP27_12065"
     CDS             <1..385
                     /locus_tag="VGP27_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7852073.1"
                     /translation="YGDAVGEAFQLRDDLLGIFGSPEITGKPAGADLTEHKATSIVVA
                     AHQMADPTLRRQLAELMNTAELDVAGVERWRSLIVATGAVERIEEMIADRLRRASEWI
                     YESRLDEAMRSALLTMASVCTQRAA"
     gene            385..1899
                     /gene="crtI"
                     /locus_tag="VGP27_12070"
     CDS             385..1899
                     /gene="crtI"
                     /locus_tag="VGP27_12070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEV7852074.1"
                     /translation="MRAIGGRTDHVVVVGAGLAGLSAALHLAGRGRAVTVVERGDVPG
                     GRVGRLDVGGYRLDTGPTVLTMPDLIDETFAAVGESTADRLELEPVDPAYRALFSDGS
                     ALDVHSDRDAMAAEVERLAGPQQAQGYLRLRDWLTRLYQVEFDGFIGSNFDSPLSLLT
                     PQLARLTALGGFRRWDRVIKRFITDPRLHRIFTFQALYAGVPPQRALAVYAVIAYMDT
                     VAGVYFPRGGMRALPDALAAAAADAGVEFRYGATVSGLDRRGDRVRAVDTDTGERIHA
                     DAVILTTELPDTYRLLGRTPRRLLSLRPSPSAVVAHIGCRSADGEMPHHHILFGDTWE
                     QTFREIIDDGVVMSDPSLLVTRPTAGDRALAPPGRDLLYVLAPAPNLSRGTVDWDSVG
                     GAYAESMVAAVTDRLPALGVDTELLHVVNPAGWAEQGMVAGTPFALAHTFGQTGPFRP
                     ANTVRGIDNVVLAGSSTVPGVGVPTALLSGRLAADRVTGAVTRNVATHIGSGHP"
     gene            1896..>2750
                     /locus_tag="VGP27_12075"
     CDS             1896..>2750
                     /locus_tag="VGP27_12075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEV7852075.1"
                     /translation="MIRSELDAAGVRDPALRDAYQQCRLLNAEHGRTFFLATRLLAPE
                     QRPAVHALYGFARRADDILDDFDAGASTAERAERLQLLATQLFNQLVQQQDYGDDPAL
                     RAVVHTARRYGIPWEMFDDFLASMRMDLTVIDYPDRKALERYMHGSAEVIGLQMLPVL
                     GTVSPREEAAPYAAALGKAFQLTNFLRDVDEDLQRGRLYLPADELAAYRVDRDILTWC
                     HTNKRTDVRVRKALTEQHAATRRVYEVARKGIEMLDPRSRPCVAAAYTLYSEILDRIE
                     NIDFAIFDQ"
BASE COUNT          443 a          972 c          893 g          442 t
ORIGIN      
        1 ttacggcgac gcggtcggcg aagcctttca gctgcgcgac gatctgctcg gcatcttcgg
       61 ctcacccgag atcaccggta aaccggcagg cgccgacctc accgagcaca aggcgaccag
      121 catcgtggtg gccgcacatc agatggccga cccgacgctg aggcgtcagc tggccgagct
      181 gatgaacacg gccgaactcg acgtagccgg cgtcgaacgg tggcgcagcc tgatcgtggc
      241 caccggcgcg gtggagcgga tcgaggagat gatcgccgac cgcttgcgcc gtgcctcgga
      301 gtggatctac gaaagccgtc tcgatgaggc gatgcgctcg gcgctgctca ccatggcctc
      361 ggtctgcacc cagcgggcgg cgtgatgcgg gccatcgggg gcagaacgga ccacgtggtg
      421 gtcgtcggcg cgggcctggc cggactgtcc gccgcgttgc acctggcagg ccgtggccgt
      481 gcggtgaccg tcgtggaacg cggcgacgtt cccggcggac gggtcggcag gctcgacgtc
      541 ggcggctatc gccttgacac cggcccgacg gttctcacca tgccagacct gatcgacgaa
      601 accttcgcag ccgtcggcga atccaccgcc gaccgactcg agctggagcc cgtcgacccc
      661 gcataccgtg ccctcttctc agacggcagc gcgctggacg tgcacagcga tcgcgacgcg
      721 atggccgccg aggtggaacg gctcgcgggt ccgcagcagg cgcagggcta cctgcggctg
      781 cgcgactggc tcacgcggct gtaccaggtc gagttcgacg gcttcatcgg ctctaacttc
      841 gactctccct tgtcgttgtt gactccgcag ctcgcccgtc tcaccgcgct cggcggcttc
      901 cgacgatggg accgggtgat caagcggttc atcaccgatc cgcggttgca ccgcatcttc
      961 accttccagg cgctctacgc aggggtacca ccccagcggg cgctcgcggt ctacgccgtc
     1021 atcgcctaca tggacaccgt cgccggtgtg tacttcccgc gcggtgggat gcgtgcgctg
     1081 ccggatgcgc tggctgccgc cgcggccgat gcgggggtcg aattccgtta tggcgcaacg
     1141 gtgagcgggc tggatcgccg cggtgaccgc gttcgggccg ttgacaccga cacaggcgag
     1201 cgcatccacg ccgacgccgt catcctcacc accgagctgc ccgacaccta ccgactgctc
     1261 ggccgcaccc cccgccgact gttgtcgctg cggccctcgc cgtccgcggt cgtcgcccac
     1321 atcggctgcc gatcggccga cggtgaaatg ccgcaccacc acatcctctt cggtgatacc
     1381 tgggagcaga cattccgcga aatcatcgac gacggtgtcg tgatgagcga tccgtctctg
     1441 ctggtgacca ggccgacggc gggagatcgg gcactggcac cacccggacg tgatctgctc
     1501 tacgtgctgg caccggctcc gaacctttcg cggggaaccg tcgactggga cagcgtcggc
     1561 ggcgcctatg ccgagagcat ggtggccgct gtcaccgatc ggctcccagc gctcggggtc
     1621 gacaccgaac tgctgcacgt cgtcaacccg gcgggctggg ccgaacaggg catggtggcg
     1681 ggcacaccgt tcgcgctggc gcacacgttc ggccagacag gaccgttccg ccccgccaac
     1741 acggttcgcg gcatcgacaa cgtcgtcctg gcaggctcgt cgacggtgcc gggcgtcggc
     1801 gttccgaccg ccctgctgtc gggacggctt gcggctgacc gggttaccgg tgccgtcacc
     1861 cgcaacgtgg caacgcacat cgggagtggt cacccatgat tcgctcagag ctcgacgccg
     1921 cgggtgtgcg cgatccagca ttgcgtgacg cctaccaaca gtgccggctg ctcaacgccg
     1981 aacacggccg aacgttcttc ctcgccacca gactgttggc gccggaacag cggcctgccg
     2041 ttcacgcgct gtacggcttc gcgcggcgtg ccgacgacat cctcgacgac ttcgacgccg
     2101 gcgccagcac cgccgaacgc gccgagcggc tccagcttct cgccacccaa ttgttcaatc
     2161 agcttgtcca acaacaggac tacggtgacg accccgccct gcgggccgtc gtccacaccg
     2221 cgcgccggta cggcattccc tgggagatgt tcgacgactt cctggcatcc atgcggatgg
     2281 acctgacggt catcgactat cccgaccgca aggcactcga gcgttacatg cacggatcgg
     2341 ccgaggtgat cgggctgcag atgctaccgg tgctgggcac tgtctctccg cgcgaggagg
     2401 ccgcgccgta tgccgccgcg ttgggaaagg cgttccagct caccaacttc ctgcgcgatg
     2461 tcgacgagga tctgcagcgt ggcaggctgt atctacccgc cgacgaattg gccgcctacc
     2521 gcgtcgaccg ggacatactg acgtggtgtc acaccaacaa gcgcaccgac gtccgggtgc
     2581 gcaaggcact caccgaacag cacgccgcca cccgccgggt ctacgaagtg gcccgcaagg
     2641 gcatcgaaat gctcgacccg cggtcgcggc catgcgtcgc agcggcatac acgctgtact
     2701 cggagatcct ggaccgcatc gagaacatcg acttcgcgat cttcgaccag
//
LOCUS       DASYLX010000501         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3754859,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000501 DASYLX010000000
VERSION     DASYLX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754859"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VGP27_12080"
     CDS             <1..957
                     /locus_tag="VGP27_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852076.1"
                     /translation="TGYGMAALGKVIVAAATAWPGVLAGRVVDRLGKGIRGAPRDAML
                     VDGVDDAARGRVFGFHRAMDTFGAVVGPLLGLAGYELLGHEIAPLLWVAVVPAVLSVA
                     LVFLARETRRVAPVQRKAIFARVGDLPRKYWRVTAFLVAFGVVNFPDALLLLRLNEIG
                     FTVVEVILAYVGYNLVYALSSYPAGLLADRISRPAMFGVGLVFFAVGYIGLGLTTDTV
                     TSWILIGAYGLFTGCTDGVGKAWISSLVGSDLQGSAQGVFQGASGFAILVAGVWAGLL
                     WGADGRLPLLVSGVVGAVFALALLAGALRYRVGTTTSTPSSA"
     gene            complement(915..1700)
                     /locus_tag="VGP27_12085"
     CDS             complement(915..1700)
                     /locus_tag="VGP27_12085"
                     /inference="COORDINATES: protein motif:HMM:NF027496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HEV7852077.1"
                     /translation="MSAGCYEVAVVGVNRAEGRLARAGVTDIAVLDDCDLSESPFAEA
                     SHTWTLPACDARVVITDQMRSGRDDLAPYLGVAVHGAPNYFMVTGVGAAADARLDYIA
                     ECLALMRRTGNTRIEVRYSTQRMFHLHGADKADDLDASYWQRMAKLAPTAFDLSSHTG
                     VLDGVYDGTATICVGDDERRVRVRLSGRLDPIDGRYHWQGTVFDAMPDTVLMQPRPVT
                     LTIGEQTAACRITERTPQGRYSVAGVGAPPYALDGVEVVVPTR"
     gene            complement(1697..2605)
                     /locus_tag="VGP27_12090"
     CDS             complement(1697..2605)
                     /locus_tag="VGP27_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEV7852078.1"
                     /translation="MTASVRPVGPTRQEFAERLLKGSVKKSYAPVVDIDWDAPLDPDK
                     FFLPPKCVSLYDTPMWHGMTREQQIELSRQELVNTLSAGIWFENILNQALLRKIMHQD
                     PTARSTHYELTELGDETRHMVMFGKAIEHVGAKPVQPRWSQRVIINALPLGFKGSVLW
                     VAALIGEEIFDSLQRQMMDDDDLQPMVQRLMRIHVTEEARHIQFARDGLRKRTPTMGR
                     ITKLWVSNINGLGGLFFRYLFTSRIPYHRVGLDADRARHLARTSPHRHEVQASGFAPL
                     AAFIEEVGLMGPISRRLWRRTGFLPQ"
     gene            2733..3782
                     /locus_tag="VGP27_12095"
     CDS             2733..3782
                     /locus_tag="VGP27_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV7852079.1"
                     /translation="MAVDKTERSRPPDPEDPRKPDSPTDLTKRSWFYVLRKTAREFQQ
                     DQCTDLAAALTYYAVLSLFPALLVLVSLLGVFGQGRRTVDAVLQMAADVVPSDAMDLL
                     RQPIEQLVQSPSAGVALITGALAALWSASAYIGAFGRAMNRIYEIDEGRPVWKLRPLQ
                     LVLTLAGLVLVAAVAIMLVISGPVAEAVGNAIGAGSVAVTAWNIAKWPVILVCVVLAV
                     AVLYYSTPNVKQPKFRWISVGAAFAILIWIAASVLFGFYVTNFGSYNKTYGTLAGVIV
                     FLLWLWITNLALLFGAELDAELERGRQLQAGLPAERDIQLPPRDTRVLDKNEKAEEKD
                     IERAEELRRTKGGEA"
     gene            3798..>4057
                     /locus_tag="VGP27_12100"
     CDS             3798..>4057
                     /locus_tag="VGP27_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7852080.1"
                     /translation="MTRRIHVIGIGAGDPDYVTAEAVRALNDTQVFFAMDKGDPKRDL
                     VALRRQICERFISEPGYRFVELADPPRANDAEYRQAVTDWHSA"
BASE COUNT          617 a         1288 c         1407 g          745 t
ORIGIN      
        1 acgggctacg gcatggcggc gctcgggaag gtcatcgtgg cggcggcgac ggcatggccc
       61 ggtgtgctgg cggggcgggt cgtcgaccgc ctcggcaagg gaatccgcgg tgcgccgcga
      121 gacgcaatgc tggtcgacgg cgtcgacgac gccgcccgcg gccgggtgtt cggattccac
      181 cgcgcgatgg acacattcgg cgctgtagtc ggcccgttgc tgggcctggc gggctacgaa
      241 ctgctcggcc acgagatcgc cccgctgctg tgggtggccg tcgtccctgc ggtgctcagc
      301 gttgcgctcg tcttcctggc acgcgagacg cggcgggtcg cgcccgtgca gcggaaggcg
      361 atcttcgccc gggtgggaga cctgccccgc aagtactggc gggtgaccgc gtttctggtc
      421 gcgttcggcg tcgtcaactt tccagacgct ctgctgctgc tgcgactcaa cgaaatcggc
      481 ttcacagtgg tcgaggtgat cttggcgtac gtcgggtaca acctcgtgta cgcgctgtcg
      541 agctatccgg ccggcctgct cgccgaccgg atttcgcggc ccgcgatgtt cggggtcggt
      601 ctggtgttct tcgcggtcgg ctatatcggg ctcgggctga ccaccgacac cgtcacctcg
      661 tggattctga tcggggccta cggtctcttc accgggtgca ccgacggcgt gggaaaagcc
      721 tggatttcgt cgctcgtcgg gtcggacctg cagggcagcg cgcagggtgt tttccagggc
      781 gccagcggct tcgcgattct ggtcgcaggc gtgtgggccg gtctcttgtg gggcgcggac
      841 gggcgcctgc cccttctggt ttccggcgtc gtcggggcgg tgttcgcgtt ggcgctcctc
      901 gcgggcgcgc tgcgctaccg ggtcggtacg acgacttcga cgccgtcgag cgcgtagggg
      961 ggcgccccga cgccggccac tgaatacctg ccctgcggtg tgcgttcggt gatccggcac
     1021 gcggccgtct gctcgccgat cgtcaaggtc accggccgtg gctgcatcag gactgtatcg
     1081 ggcatcgcgt cgaaaaccgt tccctgccag tggtatcggc cgtcgatggg atcgagtcgg
     1141 ccggacagcc gcacgcgcac acggcgctcg tcgtcgccga cgcagatcgt cgcagtgccg
     1201 tcgtagaccc cgtcgaggac gccggtgtgt gaactcaggt cgaatgccgt cggcgccagc
     1261 ttcgccatcc gctgccagta cgacgcgtcc aaatcgtcgg ccttgtctgc tccgtggagg
     1321 tggaacatgc gctgtgtgct gtaccgcacc tcgatgcggg tgttgccggt gcgccgcatc
     1381 agcgcgaggc actccgcgat gtagtcaagt cgcgcgtcgg cggcggcgcc cacacccgtg
     1441 accatgaaat agttcggcgc accgtgcacg gccactccga ggtagggcgc gagatcgtcg
     1501 cggccggaac gcatttggtc cgtgatcacg acccgtgcgt cacacgccgg cagggtccag
     1561 gtgtggctgg cctcggcgaa cggggactcg ctgagatcgc agtcgtccag aaccgcgatg
     1621 tcggtgactc cggccctggc gagccgtcct tccgcgcggt tcaccccgac gacggcgacc
     1681 tcgtagcagc ctgcgctcat tgcggcagga agccggtgcg ccgccacagc cggcgcgaaa
     1741 tcggccccat caacccgacc tcctcgatga acgcggccag cggcgcgaag ccgctcgcct
     1801 gaacctcgtg ccggtgcggg ctggtgcgcg cgaggtggcg cgcacggtcg gcgtcgagtc
     1861 caacgcggtg gtaagggatt ctgctggtga acaggtagcg gaagaacaag ccgcccaagc
     1921 cgttgatgtt ggacacccac aatttcgtga ttctgcccat ggtcggtgtt cgcttccgga
     1981 ggccgtcgcg agcgaactgg atgtgccgag cttcctcggt cacgtgaatc cgcatgagcc
     2041 gctgcaccat tggctgcaga tcgtcgtcgt ccatcatctg tcgctgcagc gagtcgaaga
     2101 tctcctcgcc gatcagcgcg gccacccaca gcacggatcc cttgaagccc aacggcagtg
     2161 cgttgatgat gacgcgttgc gaccaccggg gctgcaccgg tttcgcgccg acgtgttcga
     2221 tcgccttgcc gaacatcacc atgtggcggg tttcgtcacc gagttccgtc agctcgtagt
     2281 gcgtcgagcg ggccgtcggg tcctggtgca tgatcttgcg cagcagcgcc tgattcagga
     2341 tgttctcgaa ccagataccg gccgaaagag tgttgacgag ttcctgtcgc gacagttcga
     2401 tctgctgctc gcgggtcatc ccgtgccaca tcggggtgtc atacagcgag acgcacttcg
     2461 gcggcaggaa gaatttgtcg gggtccagcg gcgcatccca gtcgatgtcg acgaccggcg
     2521 catacgactt cttcaccgac cccttgagca gacgctcggc gaactcctga cgagtgggtc
     2581 caaccggccg caccgacgcg gtcatagtcg cctccatcca gtagacgctg tgtctaaagg
     2641 taagtaggcc ggggaaaaga tgtcaatacc cccggtaccg gatactttcg ctaacggtcg
     2701 tttgacgcga ctgtcagcgg gtagcggtcg gtatggccgt cgacaagact gaacgcagcc
     2761 gtccaccgga ccccgaggat ccgcggaagc cggactcgcc gaccgatctc accaagcggt
     2821 cgtggttcta cgtactgcgc aagacggcgc gggaatttca gcaggaccaa tgcacggacc
     2881 tcgcggcggc gctcacgtat tacgcggtgc tgtcgctgtt tccggcgctg ttggtgttgg
     2941 tgtcgctgtt gggcgtgttc ggccagggac gacgaaccgt cgacgcggtg ctgcagatgg
     3001 cggccgacgt cgtgccgagc gacgcgatgg atctgttgcg ccagcccatc gagcagctgg
     3061 tgcagtcgcc gtcggccggc gtcgcgctga tcaccggcgc gctggccgcg ctatggtcgg
     3121 cgtcggctta catcggcgcg ttcgggcggg cgatgaaccg gatctacgaa atcgacgagg
     3181 gcaggccggt gtggaagcta cggccgctgc agctggttct cacgctggcc ggcctggtgc
     3241 tggttgcggc ggttgcgatc atgctggtga tcagcggtcc ggtggccgaa gccgtcggca
     3301 acgcgatcgg tgcgggatcg gtcgcggtca cggcgtggaa catcgcgaag tggccggtga
     3361 ttctggtgtg cgtcgttctg gcggtggctg tgctgtatta ctccacgccg aacgtgaagc
     3421 agccgaagtt ccgctggatc agcgtcggcg cggccttcgc catcctcata tggatcgcgg
     3481 cgtcggtcct attcggcttc tacgtcacga acttcggcag ctacaacaag acctacggca
     3541 ctctcgccgg ggtgatcgtg ttcctgctgt ggctgtggat caccaacctg gccctgttgt
     3601 tcggcgcaga gctggacgcc gaactggaac gcggccgtca actgcaggcc ggcctgcccg
     3661 ccgagcgcga cattcaattg cccccgcgcg acacccgcgt cctcgacaag aacgagaagg
     3721 ccgaggagaa ggacatcgag cgcgccgagg aactgcgccg caccaagggc ggcgaggcct
     3781 agcgcatact cacgggcgtg acccgtcgga tccacgtcat cggtatcggg gccggcgacc
     3841 ctgattacgt gacggcggag gccgtccgcg cgctcaatga cacgcaggtg ttcttcgcga
     3901 tggacaaggg cgacccgaaa agagatctgg tggcgctgcg gcgacagatc tgtgagcggt
     3961 tcatcagtga gccgggctac cggttcgtgg agttggccga cccgccgcgc gccaacgacg
     4021 ccgagtatcg gcaggccgtc acggattggc attcggc
//
LOCUS       DASYLX010000502         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3863270,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000502 DASYLX010000000
VERSION     DASYLX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863270"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGP27_12105"
     CDS             <1..429
                     /locus_tag="VGP27_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852081.1"
                     /translation="LEDGTWRASIPARDWSERGDSEQEARDRAVEHAITDGEDADEIV
                     RNAPRGAIELEADDPRVGWIWRFTAQTEHFSDGSWRAWFPSGGWTVTGSTEQDAIEKA
                     NLEWFRRREEPDESARRIALMRRHLVEPVRGVQNTSSAVL"
     gene            complement(551..2245)
                     /locus_tag="VGP27_12110"
     CDS             complement(551..2245)
                     /locus_tag="VGP27_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852082.1"
                     /translation="MSDQRYDAPDTAVVATAAAAVVARSPRVGYVPARANTGRDGVAV
                     ALLVIALLLPWSLDFGVGVPVGNGLLIALVVAVTLLAILAALAPHVGPFRLTASDQNV
                     RRTSRIRLLLSIAYLVVAIGFVAYQLLETWRDGGTGLVPPGIGPGLLLGVGGALLAAQ
                     PPITSITLEDNGFRRWYAAARALGVLSIVLATLSVAFNVYWRLRYLFVTNVDFGGRDV
                     AVIVTTVLYGAAAMIALVIASRWLLEKSAAARLATTALGASGALAATLVWLLPIGRDI
                     DGFHGIAQNTSTAAVGYEGYLFWAAAAAIVAPTTLYAIFLVKPPTLGAYRSAAQKCLT
                     LIAFWAFAAAALRVVDFVIALSLDLPRALYDSIAMTVFNVITGLIARWLQRQLARGEL
                     VLTVIAAFSGLLFVFTVANLAIGVALAPRYAELSPDAVYGNNLAQQITSTFDVVVCAL
                     SFAVLVTMLFTGPLAGYLVRRRDAGTAAPTSPPSPSASAPPTVARSSQPAAVPKIVRL
                     REDSTTVLAAPPTEEISAPTAALRILRREPASDPTTQLPAPPKEAGEQASGPVTDG"
     gene            2392..4176
                     /locus_tag="VGP27_12115"
     CDS             2392..4176
                     /locus_tag="VGP27_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852083.1"
                     /translation="MDVVLGVAVTGRVARLALIGSSASSGQVFDEYALDLPDDATTDL
                     ADTIVGTYRAVADSGNRVIATRLCLPDASEAETLRMTVSNAGVRNVEVVTETEAATAL
                     ARRVGADAALLLSADDTVSLTVIGEDEESTTVLASVPIGSAGAAVACAAVLAQAPQAT
                     TRVMLVGQRLDLNSVAAELRSTTPVEVPPDPGFAIARGAAQTADDPWIAPAGAATQMA
                     PAAGDATQMAPAAGDATQMAPAASDATQMAPASDGAVGPLLAYSLDEPDSYEMSADSL
                     EEFVPEQDDEAPYTAVIAPPPPRTLMMGSALGFVLISFATLAVTVAVTIRPVADVQAQ
                     PVPAIQSETVPGRYLPAVPHEPDPVALPMAVVSPPPAAPALPRVPINRGPAIDNVPVA
                     PPAVPGQAPVIPAPEVPPVPLPPDVFPPWNPFPRPSWPWPTTTTTTSPTTTTTSPTTT
                     TTSPTTTTTTTTTTTTTSPTTTTTTTTPSSVIPAPKSEPQPEPEPAPAYKPPALAPQP
                     APAPVAPAPAPVPVAPAPAIAPAPAVAPAPAPAPAIAPAPALAPAPAPVIPEAPSGGS
                     GSSSGGSGSSSGGGGAGQAPATTPVLSP"
     gene            4212..>5420
                     /locus_tag="VGP27_12120"
     CDS             4212..>5420
                     /locus_tag="VGP27_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7852084.1"
                     /translation="MFGKPSARKTTAVGAAVLALATACSRADAPVPTATEQTPQAQVV
                     QAPAPVPTTTSPPEAPPNPAFDKVSELINRAIAANELPGAVVLVGHGGKVVFHRAYGD
                     RKLAGEPGLDGSPAPAERMTEDTIFDLASLTKPLATAPAVMQLCEQGKVAFDDPVQKY
                     LPDFNPANDPVRAQVTVRMLLTHTSGETGDVELKDPWGLGQIDRAEGFHRALTTPLES
                     TPGAGVRYSDINFILLGAIVEKLTGETEDDYVQQNVFAPLGMNETRYLPAGKVCGPHV
                     MRGAALAWAPQTAGFAPAGCPADTWSIGLLSRIAPTARDEENRGDPGKNPDIDHLLRG
                     SVDDTTARRMGGVAGHAGVFSTAHDVGVYAQALLDRLAGRPSTYPLTQATVELMTSPQ
                     QPGHSAGQVEA"
BASE COUNT          898 a         1807 c         1961 g          754 t
ORIGIN      
        1 ctggaggacg gcacgtggcg cgcctccatc ccggcacggg actggtcgga gcgcggcgac
       61 agcgagcaag aggcgaggga ccgagccgtc gagcatgcga tcaccgatgg tgaggacgcc
      121 gacgaaatag tgcgcaatgc cccacgtggc gctatcgagc tcgaagcgga cgatccgagg
      181 gtgggctgga tctggcggtt cacggcccag acggaacact tcagcgatgg cagctggcga
      241 gcgtggtttc cgagcggagg atggaccgtc acgggatcga cggaacagga cgccatcgag
      301 aaggcgaact tggagtggtt ccgccgccgg gaggaaccgg acgaaagtgc tcgccggatc
      361 gccttaatgc gtcgccatct tgtcgagccg gtgcgcggtg tgcagaacac gtccagtgcg
      421 gtgctgtagt cggcgtggaa cgatccgaat cccgcagact cagtgcgatc gatcattgaa
      481 gggctggacg gccagaggtg acgcgtacgt cccgaaccgc ctcgaaggcg ggcagaacga
      541 cgcggcttgg tcagccgtcg gtgacaggcc ccgacgcttg ctcgcctgct tccttcggcg
      601 gggcgggcag ctgcgtcgtc gggtcagatg ccggttcgcg acgcaggatg cgaagcgcgg
      661 cggtaggcgc ggagatttcc tccgtcggcg gcgctgcgag caccgtcgtc gaatcctccc
      721 tcagccgaac gattttcggg actgcggcgg gttgcgaaga acgcgcgact gtcggcggcg
      781 ccgaggctga tggcgacggc ggggaagtgg gggccgcggt cccggcgtcg cgccggcgta
      841 ccaggtagcc cgccagcggc cccgtgaaca gcatggtgac cagcacggcg aagctcagcg
      901 cgcagaccac cacgtcgaac gtgctggtga tctgctgggc caggttattg ccgtagacgg
      961 cgtcgggcga cagttcggcg taacgggggg cgagcgcgac gccgatggcc aggttcgcga
     1021 ccgtgaagac gaacagcaac ccgctgaacg cggcgataac ggtcaatacg agctcgcccc
     1081 tggcgagttg gcgctgcagc cagcgcgcga tcaagccggt gatgacgttg aacaccgtca
     1141 ttgcgatgct gtcgtacagg gcgcggggca gatccagcga caaggcgatc acgaagtcga
     1201 cgacgcgcag cgcggccgcg gcgaacgccc agaacgcgat gagcgtcaga catttctgcg
     1261 cggccgagcg gtaggcgccc agcgtcggcg gcttgaccag gaagatggcg tagagcgtcg
     1321 tcggtgcgac gatcgccgcg gcggccgccc agaacaggta gccctcgtaa ccgacggcgg
     1381 ccgtcgaggt gttctgcgcg atgccgtgga agccgtcgat gtcacggccg atgggaagga
     1441 gccacaccag cgtggccgcc agcgcgcccg aggcgccaag ggcagtggtg gccaaccgcg
     1501 ctgcggcgga cttctccagg agccagcgcg acgcgatgac caacgcgatc atcgccgccg
     1561 cgccgtagag cactgtggtg acgatgaccg cgacgtccct gccgccgaag tcgacgttcg
     1621 tgacgaacag ataccgcagc cgccagtaca cgttgaaggc gaccgacagc gtcgcgagca
     1681 caatcgatag cacacccagc gcccgcgcgg cggcatacca gcgccggaag ccgttgtctt
     1741 cgagcgtgat actcgtgatc ggtggctgcg ccgccagcag cgcgcccccg acgcccagca
     1801 gcagtccggg gccgatcccc ggcggtacca gcccggtccc gccgtcgcgc caggtctcca
     1861 acagctgata cgcgacgaag ccgatcgcga cgaccagata ggcgatgctg agcagcagcc
     1921 ggatccggct tgtgcggcgg acattctggt cggatgcggt gagccggaac gggccgacgt
     1981 gcggcgcgag cgcggcgagg atggcgagca gtgtgacggc gacgacgagg gcgatcagca
     2041 gcccgttgcc gacggggaca ccgacgccga agtcgaggct ccacggcaga agcagcgcga
     2101 taacgagcag cgcgactgcg actccgtcgc gcccggtgtt ggcgcgggcg ggcacgtaac
     2161 cgactcgggg cgaccgggcg acgacggccg cggcggcggt cgccaccacg gcggtgtctg
     2221 gcgcgtcgta acgctggtcg ctcacgtgac gatttcctct ccagagtgac gattcgagtt
     2281 gctgccaccc cagcgtgcca ttttcgcatc cgcgcacaag cggttcgggc gtcggctcgg
     2341 ccgccacggg cgcggtagct ttggatcttt gatgaggcaa ggagtgaacc tgtggacgta
     2401 gtcctcgggg tggcggtcac cggacgggtg gcgcgtctgg cgctgatcgg ttcgtccgcc
     2461 tcgagcggcc aggtgttcga cgagtatgcg ctcgacctgc ccgatgacgc gacgaccgac
     2521 ctcgccgaca ccattgtcgg cacctaccgt gcggtggccg actcgggcaa tcgtgtgatt
     2581 gcgacacggc tctgtctgcc cgacgcgtcg gaggccgaga ccttgcgcat gaccgtgtcg
     2641 aacgcgggcg tgcggaacgt cgaagtggtg acggagaccg aggcggccac ggcgctggcc
     2701 cgcagagtcg gcgcggacgc cgcgttgctg ctgtcggccg acgacaccgt gtcgctgacg
     2761 gtgatcggcg aggacgagga gtcgacgacg gtgctggcgt cggtgcccat cggctcggcc
     2821 ggtgcggccg tcgcgtgtgc cgccgtgctg gcgcaggcgc cgcaggcgac cacccgggtg
     2881 atgctggtgg ggcagcgctt ggacctgaat tccgttgcgg ccgagcttcg ttcgacgacg
     2941 ccggtggagg tgccgccgga tcccggcttc gcgatcgcgc gaggcgccgc gcagacggcc
     3001 gacgatccct ggatcgcccc ggcgggcgct gcgacgcaga tggcgccggc cgccggcgac
     3061 gcgacgcaga tggcgccggc cgccggcgac gcgacgcaga tggcgccggc agcatcggac
     3121 gcgacgcaga tggcaccggc ttctgatgga gcagtcgggc cgctgctggc ctactcgctg
     3181 gacgagccgg actcctacga gatgtccgcc gactcgctcg aggagttcgt gccggagcag
     3241 gacgacgagg cgccgtacac cgcggtcatc gcgccgccac cgccgaggac actgatgatg
     3301 ggcagtgcgc tggggttcgt gctgatcagc ttcgcgacgc tggccgtcac ggtcgcggtc
     3361 accatccggc cggtcgccga cgtgcaggcg cagccggtac cggccatcca gtcggagacg
     3421 gtgccggggc gttacctgcc tgcggtgccg cacgagccgg atccggtcgc cctgccgatg
     3481 gcggtggtgt cgccgccgcc tgcggccccg gcgctgccca gggttccgat caaccgcggc
     3541 ccggcgatcg acaacgtgcc ggtggccccg ccggcggtgc ctgggcaggc gcctgtgatt
     3601 ccggctcctg aggtgccgcc cgtcccgctt ccgcctgacg ttttcccgcc atggaacccg
     3661 ttccccaggc cgtcgtggcc gtggccgacg acgacgacca ccacgtcgcc gacgaccacc
     3721 acgacgagtc ccacaaccac cacgacgagt ccgacaacta cgactacgac gacaacgacg
     3781 accaccacca cgtcgccgac aaccacgacg accacgacga cgccgtcgtc ggtcatcccg
     3841 gcgccgaagt cggagccaca gcccgaaccc gagccggcgc ccgcgtacaa accgcctgct
     3901 ctcgcgcccc agccagcgcc tgcgccggtg gcgccggccc ccgcacccgt tcccgtggcg
     3961 ccggcaccgg caatcgcgcc tgctcccgcg gtcgcccccg cacccgcgcc agcccctgcg
     4021 atcgcacctg cgccggcgct ggctcctgca ccagctcccg tcataccgga ggcgccgtcg
     4081 ggcggcagtg ggtcctccag cggtggaagc ggatcctcca gcggaggtgg cggtgccggt
     4141 caggcgcccg cgacgacgcc ggtgttgtcc ccgtaatcca tcgccgccga gcagtgacaa
     4201 gattgagcgt catgttcggc aagccgtctg cgcgaaagac caccgcggtt ggcgccgccg
     4261 tgctcgcact cgctacggcc tgctcacgcg cggatgcacc ggtcccaacc gccaccgagc
     4321 agacaccgca ggcccaagtc gtgcaagcac cggcgccggt tcccacgacg acgtcgccgc
     4381 ccgaagcgcc tccgaatccg gccttcgaca aagtctccga gctgatcaac cgcgccatcg
     4441 cggcgaacga gttgcccggt gccgtggttc tggtcgggca cggcggcaag gtcgtcttcc
     4501 atcgggcgta cggcgaccgg aagctcgcgg gggagccggg gttggacggc tcgccagcac
     4561 cggcggagcg gatgaccgag gacacgatct tcgacttggc gtcgttgacg aagccgctcg
     4621 cgacggcgcc cgccgtcatg cagctgtgcg agcagggcaa ggtggcgttc gacgatccgg
     4681 tgcagaagta tctgccggac ttcaacccgg cgaatgatcc ggtgcgggca caggtgacgg
     4741 tgcgcatgtt gctgacgcac acgtcgggcg aaacgggtga cgtcgagctc aaggatccat
     4801 ggggcctagg ccaaatcgac agggcagaag gctttcatcg ggcactgacc acgccgttgg
     4861 agtcgacacc gggcgcaggc gttcgctact ccgacatcaa cttcattctg ctgggtgcga
     4921 tcgtcgagaa gctcacgggc gagacggaag acgactacgt ccagcagaac gtctttgcgc
     4981 cgctgggcat gaatgagacc cgctatctgc ccgcgggcaa ggtgtgtggt ccgcacgtga
     5041 tgaggggtgc ggcgcttgcg tgggcgccgc agacagcggg attcgcgcct gctgggtgcc
     5101 cggcagacac atggagcatc ggcctgttgt cgcggatcgc gccgacggcg agggacgaag
     5161 agaacagagg agaccccggc aagaatcccg acatcgacca tctgctgcgg ggctcggtgg
     5221 acgacacgac ggcgcggcgg atgggcggag tggccggcca tgcgggtgtg ttctcgacgg
     5281 cacacgatgt cggcgtctat gcgcaggcgc tgctcgaccg gcttgcgggg cggccgagca
     5341 cgtatccgct gacgcaggcg accgtcgagt tgatgacgag tccgcagcag cccgggcata
     5401 gcgctgggca agtcgaggcg
//
LOCUS       DASYLX010000503         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2328232,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000503 DASYLX010000000
VERSION     DASYLX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328232"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VGP27_12125"
     CDS             complement(<1..699)
                     /locus_tag="VGP27_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7852085.1"
                     /translation="MTHEHQPVVALRHISKTFGAVRALHDVDLTIRRGEVHGLLGENG
                     SGKSTLIKILSGFHAPDGGSELEINGRPVSVPLYPGQFRSLGMSFVHQDLGLVPSLSV
                     VENLLVGDLTSQRSWRLSWARERRRAQETFRSFGLKLDPRAKVSDLRPTDRALLAIVR
                     AVEEIRANTADDGSEYGLLVLDEPTVFLPASDRDELFAIVREIAATRASILFVSHDLD
                     EILEITDRVTVLRDG"
     gene            complement(696..1013)
                     /locus_tag="VGP27_12130"
     CDS             complement(696..1013)
                     /locus_tag="VGP27_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7852086.1"
                     /translation="MSWRDARVLLGRGIGERIGYGSSPAIVVVDMTRAFTDPSYRVGS
                     DQPDTVEAIAQVLAAARERGDVPVFFTTMPTKPMGSKYGDVVSVAEVLEYLPKDSVEE
                     AAE"
     gene            complement(1114..1272)
                     /locus_tag="VGP27_12135"
     CDS             complement(1114..1272)
                     /locus_tag="VGP27_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852087.1"
                     /translation="MTFPYVLRKGAIYEPVFWLLGPQASIYDELGIVPQRTGTPPLGR
                     SPQRVPSN"
     gene            1283..>2108
                     /locus_tag="VGP27_12140"
     CDS             1283..>2108
                     /locus_tag="VGP27_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7852088.1"
                     /translation="MSRTSQLVQFLDLSLDAFCIAGFDGYLKYANPAFARILGYTQEE
                     LLARPFMDNVHPDDAESVGAVFIGPAEGKDTSGFECRLICTDGSVRRFKWNTHTRPDE
                     GVVYGVARDVTDRRGASAELNALHRVATLVAEGVQPQDLFAIVAEEVSRVVDVSAVSV
                     MRYELDGTATECANFDRGEKLFPVGVRWSIEGTNILRLIRDSAAAARIDDYAGLKGEM
                     AAIIRSTGIRATVGVPIVVAGRLWGTIVVSTTEPDPLPEDTASRLADFTELLATAIE"
BASE COUNT          365 a          681 c          706 g          356 t
ORIGIN      
        1 cccgtctcgc agcaccgtga cgcggtcggt gatctcgagg atctcgtcga ggtcgtggga
       61 gacgaacagg atgctggcgc gcgtcgcggc gatttcccgg acgatcgcga agagctcgtc
      121 gcggtcgctt gccggcagga agaccgtcgg ctcgtcgagg acgagtagcc cgtattcgga
      181 cccgtcatca gcggtgttcg cacggatctc ctcgaccgct ctgacgatcg cgagcagcgc
      241 gcggtcggtg gggcgcaggt cggagacctt cgcgcgcgga tcgagcttga gcccgaagga
      301 cctgaaggtc tcctgtgccc tgcgccgttc gcgcgcccag gagagccgcc aactgcgctg
      361 ggaggtcagg tcaccgacga ggaggttttc gacgacagac agcgagggga cgagcccgag
      421 atcctggtgc acgaagctca tgccgagact gcggaactga cccgggtata gcgggaccga
      481 gacgggccgg ccgttgatct cgagctcgct gcctccgtcc ggtgcgtgga agccggagag
      541 gatcttgatc agggtcgact tgcccgaacc gttctctccg agcaagccat gcacctcgcc
      601 gcgccgaatg gtcagatcga cgtcatgcag tgcgcgaaca gccccaaacg tcttcgagat
      661 gtgcctgagc gcgacgaccg gctgatgctc gtgcgtcact cggctgcctc ttccaccgaa
      721 tccttgggaa ggtactcgag gacctcggcg acgctgacca cgtcaccgta cttcgagccc
      781 atcggcttcg taggcatcgt ggtgaagaac acgggcacgt cgccgcgctc cctcgcagca
      841 gcgagcactt gggcgatcgc ttcgactgtg tccggctggt cggacccgac ccggtagctc
      901 gggtccgtga atgcgcgcgt catgtcgacg acgacaattg cgggcgaact cccatagccg
      961 atgcgttcgc cgatcccccg acccagtaga actcgcgcgt ctctccagct cactggtcga
     1021 tcgaccccgc ctcagtcact ctggatcctc tccagtcgag ggtactggcg ccgggacgtc
     1081 cgcgacgacg cggacagccc gagccagcgg ccgtcagttg cttggtacgc gttgcgggga
     1141 gcggccaagg ggcggcgttc ccgttcgctg cggcacgatc ccgagctcgt catagatcga
     1201 ggcctgcggg ccaaggagcc agaacaccgg ctcgtagatc gcgccctttc gcaagacgta
     1261 cggaaaggtc acactaggca gcgtgagtcg tacgagccag ctcgtacagt tcctcgacct
     1321 atcgctcgat gcattctgca tcgcagggtt cgacgggtac ttgaagtatg ccaatccagc
     1381 gttcgcgcgc atcctcgggt acacgcagga ggagctgctg gccaggccgt tcatggacaa
     1441 tgtccacccc gacgatgccg agtccgtcgg cgcggtcttc atcgggcccg cggagggcaa
     1501 ggacactagt gggttcgagt gtcgcctaat ctgcaccgac ggctcggtgc gcaggtttaa
     1561 gtggaacacg cacacccgac cggacgaagg tgtcgtgtac ggagtggcga gggacgtcac
     1621 ggaccggcgc ggagccagcg ccgagctgaa cgcgctgcac cgcgtcgcga ccctcgtggc
     1681 ggagggcgtg cagccgcagg acctcttcgc cattgtcgcc gaggaggtct cccgcgtcgt
     1741 cgatgtctca gccgtgagcg tcatgcggta cgagctcgac ggcaccgcaa ccgagtgcgc
     1801 caacttcgac cggggagaga agctgttccc tgtcggagtc cggtggtcga tcgaaggcac
     1861 gaacatcctt cgactcatcc gtgacagcgc cgcggctgcc cgcatcgacg actacgcggg
     1921 gctgaagggt gagatggcgg cgatcatccg tagcaccggg atccgggcga cggtcggagt
     1981 tccgatcgtc gtcgccggtc gtctctgggg aacgatcgtc gtatccacca cggagccgga
     2041 tccgttgccg gaagacaccg cgtcgcgtct cgccgacttc accgagctgc tcgcgaccgc
     2101 gatcgaga
//
LOCUS       DASYLX010000504         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1560920,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000504 DASYLX010000000
VERSION     DASYLX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560920"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGP27_12145"
     CDS             complement(<1..375)
                     /locus_tag="VGP27_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7852089.1"
                     /translation="MSVFTGLKTKWAKTSPFRVLPREQWASQRPTYKDAEPAIIDAAL
                     QRSQRRPSGNWFAFAASDAIRDRPFGTSVGGLDMVAWRDTDSTLHVGPATCPHLGADL
                     ATGTVDRGALICPWHGLRLEGGR"
     gene            complement(372..>1735)
                     /locus_tag="VGP27_12150"
     CDS             complement(372..>1735)
                     /locus_tag="VGP27_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852090.1"
                     /translation="EPYLGGRVGGWTDKAHDGTDLPMNRGFHAFFRQYYNLRDMLRRI
                     DSGLGMLTAVEDYPLVDGQGRRDTFRGLPQKPPLNALAFALRSPTFRLRDLARLNARA
                     AAPLAAVSVPDIYQRLDHLDADAFLRAINFPAAARHLAFEVFSRSFFAEPGALSAAEL
                     ATMFHIYFLGSSEGLIFDVANANFDVALWNPLRAYLESRGVRFHTGVSVTAVSTDPAL
                     RVHSDSGTDFDCDGVVLATDVAGLQRIVVGSPSLGDDAWRARVEAMGVAPPFVVQRLW
                     LDRLVLADRAPFLGTGGRPPLDNVSVLERYEREATAWSRRTSGSVVELHSYAVTEPRE
                     GLGDRMRARLYELYPEIRDAEILHERVLCRSDCPRFAPGDHATRPTVTTPHRGVMLAG
                     DGIRIDLPVALMERAATTGWSAANHLLGHYGLAGHVLHTVPVQGRSGALRRLAQRDKQ
                     LVR"
BASE COUNT          287 a          584 c          608 g          256 t
ORIGIN      
        1 gcgaccacct tcgaggcgca gtccgtgcca cgggcagatg agcgcgccgc gatccaccgt
       61 gcccgtcgcc aggtccgcgc cgaggtgcgg gcatgttgca ggcccgacgt gaagggtgct
      121 gtcggtgtcg cgccacgcca ccatgtcgag cccgccgacc gatgtgccga acgggcggtc
      181 gcggatcgcg tcgctggcgg cgaacgcaaa ccagttgccg ctgggtcggc gctgcgatcg
      241 ctgcagcgcg gcgtcgatga tcgcgggctc ggcatccttg taggtgggcc gctggctggc
      301 ccactgttcg cgcggaagca ccctgaaggg cgacgtcttc gcccatttcg ttttcagacc
      361 ggtgaacacg ctcatcgcac cagctgttta tcccgctgcg ccagccgccg caaagcgccc
      421 gaccggccct gcaccggcac ggtgtgcagg acatggcccg ccagcccgta atggccgagt
      481 aggtggttcg ccgccgacca gccggtcgtt gcggcgcgtt ccatcagtgc gacgggcagg
      541 tcgatccgaa tgccgtcacc ggccagcatg acgccccgat gcggagtcgt gacggtcggc
      601 cgcgtggcgt gatcgcctgg tgcgaaccgc gggcagtcgc tgcggcacag caccctttca
      661 tgaaggatct cggcatcccg gatctccggg tagagctcgt acagtcgggc gcgcatgcgg
      721 tcgccgagac cctcgcgcgg ttcggtcacc gcgtaagaat gcagttcgac gaccgaaccg
      781 gaggtgcgcc gggaccaggc cgtggcttcc cgctcgtagc gttcgagcac actgacattg
      841 tccagcggcg gccggccgcc tgtcccgaga aagggtgcgc gatctgcaag tacgagccga
      901 tccagccaca gccgctgcac cacgaacggc ggtgcgactc ccatcgcctc gacccgcgcc
      961 cgccatgcgt catctccgag cgaaggcgac ccgacgacga tgcgctgcag gcctgccaca
     1021 tcggtggcca aaaccactcc gtcgcagtcg aagtccgtgc cggaatcgga atgcacacgc
     1081 agggcggggt cggtgctcac cgccgtgacc gatacaccgg tgtggaatcg cacgccgcgc
     1141 gactccagat acgcgcgcaa cggattccac agcgcgacat cgaagttggc gttcgccacg
     1201 tcgaagatca ggccctcgct ggagccgagg aagtagatgt ggaacatggt ggcgagctcg
     1261 gcggcggaca acgcgcccgg ttcggcgaag aagctgcgcg agaacacttc gaacgccaga
     1321 tggcgggcgg ccgcgggaaa gttgatggcg cgcaggaagg cgtccgcgtc gaggtggtcg
     1381 agccgctgat agatatcggg caccgacacc gctgccaacg gcgcggcggc gcgggcgttg
     1441 agccgggcca gatcccgcag ccggaacgtc gggctgcgca aggcgaacgc caacgcgttc
     1501 agcggcggct tctgcggcag tccgcggaac gtgtcgcgcc taccttgccc gtcgaccagt
     1561 gggtagtcct cgacggcggt gagcatcccc agcccggagt cgatgcggcg caacatgtcc
     1621 cgcaggttgt agtactggcg gaagaatgca tggaagccgc ggttcatcgg caggtcggtg
     1681 ccgtcgtggg ccttgtcggt ccacccgccg acgcggccgc cgaggtaggg ctcgc
//
LOCUS       DASYLX010000505         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2437451,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000505 DASYLX010000000
VERSION     DASYLX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437451"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGP27_12155"
     CDS             complement(<1..477)
                     /locus_tag="VGP27_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEV7852091.1"
                     /translation="MSFHSLGREELQAQHESQQANYAELQAKRLKLDLTRGKPSPAQL
                     DLSNPLLSLPGDDYRDGEGTDTRNYGGLHGLPELRGIFGELLGIPVQNLIAGNNASLE
                     LMHDVVVFSLLHGGVDSSKPWIREPAVKFLCPVPGYDRHFAITESLGIEMINVPMLE"
     gene            complement(499..593)
                     /gene="ffs"
                     /locus_tag="VGP27_12160"
     ncRNA           complement(499..593)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGP27_12160"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            662..747
                     /locus_tag="VGP27_12165"
     tRNA            662..747
                     /locus_tag="VGP27_12165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Ser,seq:gga)
     gene            822..1658
                     /locus_tag="VGP27_12170"
     CDS             822..1658
                     /locus_tag="VGP27_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852092.1"
                     /translation="MSRKIAVAWHATFSIAAGVLYFFFILPRWPELRGTTSHTLGTVL
                     RIVAGALIGLAGLPVVFTLLRTRKPELGTPQLALSLRTSSVGLHVLAGVLIIGTAISE
                     IWLSLDSAGRWLFGVYGAAAAVALLGVFAFYLAFVAELPPPPPKPLKPKAAKKPSRRR
                     RKAAEEAAADSEDAVESTTADDDATTGEATESEQSAEPAEVTAETSEDDAKAEEAPPE
                     AETESEAVSGPESESEAQAEASDEDNGAESTNGGLRNRRSPGKIGLRKRKRSRGGVAL
                     DD"
     gene            1729..2742
                     /locus_tag="VGP27_12175"
     CDS             1729..2742
                     /locus_tag="VGP27_12175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV7852093.1"
                     /translation="MARLHPCRLLIALVAVMALVAPIRPAIASAEPLDTAIAAVEPAV
                     VQIDTTIDYQHAIGAGTGFLLNPNGEVLTNFHVVQGADVITGHNVGTGQSFGADLVGY
                     DRTHDIALLQLRGASGLPVAPLGNSSSIAIGEPAVAIGNANPGSPPTREPGSITRLDR
                     TIDAKDALTGSSDELNGLIEVAAPVRAGDSGGPLVNGAGQVIGVITAATVNYRFAPGG
                     KGFAIPINQALGIADQIRSRTMSDTVHVGPPTLLGVGVAAGDQEADVPGVAIREALRG
                     GPAEQAGLVNGDVLTSVGGVSLDSATSLTQVLDRHYPGDVVDLTWIDKSGQQRTGKAT
                     LTS"
BASE COUNT          481 a          940 c          911 g          444 t
ORIGIN      
        1 ctcgagcatc ggaacgttaa tcatctcgat gccgaggctc tcagtgatcg cgaagtgccg
       61 gtcatagccg ggcaccgggc agaggaactt cacggccggc tcccggatcc acggcttcga
      121 cgaatcgacg ccgccgtgca gaagcgagaa gaccacgacg tcgtgcatca gctccaggct
      181 ggcgttgtta cccgcgatca gattctgcac cgggatgccg agcagctcgc cgaagatgcc
      241 ccggagttct ggcaggccgt gcaggccgcc gtagttgcgg gtgtcggtgc cctccccgtc
      301 gcgatagtcg tctcctggca ggctcagcag cgggttggac aggtcgagct gtgccggcga
      361 cggcttgccg cgggtcaggt cgagtttgag ccgcttggcc tgtaactcgg cgtaattggc
      421 ctgctgggat tcatgctgcg cctgcaattc ctcacggccg agcgagtgaa acgacacgat
      481 gggtcctcaa ggtctgaacg aaggggaccc cgcgcacccg acagagccca ttgacccttg
      541 ctgccttccg gccctggggg agttcacagg atagacgccg cgcggggtcc acagcgagtg
      601 taatacgtgg tggagaaccg actggacggg cgtattaggc aggtcggtta ccatggcccg
      661 cggaggattc gcctagtggc ctatggcgct cgcctggaac gcgggttggg ttaatagccc
      721 tcgcgggttc aaatcccgca tcctccgcac tcggtccgag gaacgaccag cgggcagtga
      781 tgagctggtc gctcctcggt cctaaaaatt cgaggaggag tatgagccgc aaaattgcgg
      841 tggcctggca cgcgacgttc tcgatcgccg ccggcgttct gtactttttc ttcatcctgc
      901 cgcggtggcc ggagctgagg ggcacgactt cgcacacgct tggcaccgtg ctgcgcatcg
      961 tcgcaggtgc actgatcggc ctggcggggc tgccggtggt gttcacgctg ctgcgtaccc
     1021 gcaagccgga gttgggcacg ccgcagctgg ccctgagcct tcgcacgtcg tcggtcggcc
     1081 tgcatgtgct ggcaggcgtg ctgatcatcg gcaccgcgat cagcgagatc tggctcagct
     1141 tggacagcgc cggacgctgg ctgttcggtg tctacggtgc cgccgcggcg gttgcgctgc
     1201 tcggcgtgtt cgcgttctac ctggcattcg tggccgaact gccgccgcct cccccgaaac
     1261 cgctcaagcc gaaggctgcg aagaagccga gccgacggcg ccgcaaggcc gccgaggagg
     1321 cggccgctga cagcgaggac gccgtcgagt ccaccacagc ggacgacgac gcgaccaccg
     1381 gcgaagccac cgaatcggag caatccgcgg agcccgccga ggtcacggcc gaaacctccg
     1441 aggacgacgc gaaggcggag gaggccccgc ccgaggccga aaccgaatcc gaggccgtat
     1501 ccggacccga atccgaatcc gaagcccagg ccgaggcctc cgatgaggac aacggcgcgg
     1561 aatcgaccaa cggcggtctg cgcaaccggc gttcaccagg aaagatcggc ctgcgcaagc
     1621 gcaaacggtc gcgtggcggc gtcgctctgg acgattagcc cctcgccgac aagggaacac
     1681 tcattgccgc gatgtaatga gattccgccc caagccgaaa gttcccatat ggcccgctta
     1741 cacccgtgtc gcttgctgat cgcgctcgtc gccgtaatgg cactcgtcgc gccgatccgc
     1801 ccggcgatcg cttctgcgga gccattggac actgcgatag cggcagtcga gcccgccgtc
     1861 gtccagatcg acaccaccat cgactaccag cacgcgatcg gcgcgggcac cggcttcctg
     1921 ctgaacccca acggcgaggt ccttaccaac ttccatgtgg ttcagggcgc cgacgtcatc
     1981 accggacaca acgtcgggac cggccagtcc ttcggagccg atctcgtcgg ctacgaccgc
     2041 acgcacgaca tcgcccttct gcagcttcgc ggcgcatccg ggttgccggt cgcgccgttg
     2101 ggcaactcgt cctcgatcgc catcggagag cccgccgtgg cgatcggcaa cgccaacccg
     2161 ggcagcccgc cgacgcgtga accaggcagc atcacgcggc tggaccgcac gatcgacgcc
     2221 aaggacgcgc tcaccggtag ctccgacgaa ctgaacggct tgatcgaggt tgcggcgccc
     2281 gttcgcgcgg gcgactccgg tggaccactc gtcaacggcg cgggccaggt gatcggcgtt
     2341 atcaccgccg cgaccgtgaa ttaccggttc gcgcccggcg gcaagggttt cgccataccc
     2401 atcaaccagg cgctgggcat cgccgaccag atccgttcgc gcacaatgtc cgacaccgtg
     2461 cacgtcggtc ccccgacgct gctcggcgtc ggcgttgccg cgggcgacca ggaggccgat
     2521 gtgccgggcg tcgccattcg cgaggcgctg cgcggcggtc ccgccgagca ggccggcctc
     2581 gtcaacggcg atgtcctcac cagcgtcggc ggcgtatcgc tcgactcggc gacgtcgctg
     2641 acccaggtgc tcgacaggca ctaccccggc gatgtcgtcg acctgacctg gatcgacaag
     2701 agcggccagc agcgcaccgg caaggcgacg ctgacttcct gaccgtaaac gtcaattttg
     2761 tggccgaaag gcaccg
//
LOCUS       DASYLX010000506        14255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2217299,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000506 DASYLX010000000
VERSION     DASYLX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217299"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VGP27_12180"
     CDS             complement(<1..719)
                     /locus_tag="VGP27_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7852094.1"
                     /translation="MDRDQLVDLTRRALKLARDKTTDLAPAECRVAADTYTSADRHAR
                     DVQMVLTSPQLVGYVSELPKPGSYCTKTVMGRSILLTRTSDGTVKAFENVCLHRQSQI
                     TEGCGSARRLVCPYHAWNYDLDGNLVGIPGKEGFPESSTGSAKLSELPATECAGFLWV
                     ALDPHATLDIREFLGPLADELDSWGIGRWAPLGEKVLDTAINWKLAIDTFAENYHFAT
                     VHKTTFATIARSNCTVFDSFGP"
     gene            complement(785..1606)
                     /locus_tag="VGP27_12185"
     CDS             complement(785..1606)
                     /locus_tag="VGP27_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7852095.1"
                     /translation="MVSASATGRVAGKRVLITGAARGLGRSHAVRLAEEGADLVLVDL
                     CRSLPEIEYPLATEDDLAETVRLVSDLGRRCVSHVVDVRDEAALRSAVDDGVAELGGL
                     DGAVANAGVLTGGPWHQTTSEDWRTVVDVNLIGVWNTCAASIPHLLEQGGGSLVNISS
                     AGAIAGFPLQVPYTAAKHGVIGLTLSLANELAAQSVRVNSVHPTGVPTGMLPSSLGVA
                     LGGERSDLVPLFINAMPTPCVEPVDVSNAVLFLISDESRFVTGLQFKVDAGVTIN"
     gene            complement(1600..2673)
                     /locus_tag="VGP27_12190"
     CDS             complement(1600..2673)
                     /locus_tag="VGP27_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7852096.1"
                     /translation="MRRVVQFSTGNVGVHSLKAIIGRPDLELVGVHAASPKKIGRDAA
                     ELCGLTEPTGIIATDDIDALIALKPDCVVYTAQGETRPMEVIVQMSKFLSAGINIAAT
                     SMVWLVTPRQVDDWLRVPLEQACEAGNASLYVNGIDPGYSGDTEVHSALSLTTRVRSI
                     TVQEIFDYANYDDFEFTGKSMGFGVSADEATPMLFLPGVLTTMWGGAVRNLADQLGVQ
                     LDELRQRIEPWYTDNKIVCKMATVEPGEMAAVKFAVEGVRDGVPVITMEHITRLTDAS
                     APQWAYPPDGHPGVHRVLVEGDPRVELNTHVAHPGLDVTEAGCLSTAARVVNAIDWIC
                     RAPQGLIAVEDIPQAEIIRGLMW"
     gene            complement(2691..3478)
                     /locus_tag="VGP27_12195"
                     /pseudo
     CDS             complement(2691..3478)
                     /locus_tag="VGP27_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            3581..4183
                     /locus_tag="VGP27_12200"
     CDS             3581..4183
                     /locus_tag="VGP27_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852097.1"
                     /translation="MAVVDKPSARETKRLQTRERLMGAAIAEFKRGGMAEADVGAIVA
                     AAGVAHGTFFFHFPTKEHVLLELEQREEERIAKQLARSTGPESDLVSTLNETVRLVMG
                     LERRLGAVLFKDFLALHFSQTRPSLDESRQHPVIVKVAQEIELAQKRGAVDPDVTPMN
                     SAVFFLLGLYALLTTTHDWPGQAAMIEDYVARTLRGIAVK"
     gene            4308..4955
                     /locus_tag="VGP27_12205"
     CDS             4308..4955
                     /locus_tag="VGP27_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852098.1"
                     /translation="MAASQAAQRQADKWLISGSILIGTAALGIFGLPLFLRGVWLLRK
                     AQRDGLTVRPMLVTLIGYLVIIDAAINTVGWALDLVANHTILARILLSGWGAMFDAGY
                     FWHYNELWLGGAAGPGEKAMEVGMILTVFTMRIAAAIGFLQMKRWGQQWMIITCWMGV
                     LIWCLYVFNMTMYADVRFAGVVLPVVGWWLYDIFYITPFLAIPYLHSVNREILSD"
     gene            5012..5614
                     /locus_tag="VGP27_12210"
     CDS             5012..5614
                     /locus_tag="VGP27_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852099.1"
                     /translation="MTPQVKQSARQLRRLQTRERILGAAIAEFKLAGMSGADVNAIAT
                     AAGVAHGTFFFHFPSKEHVLLELESREEGRMAAEFSHFLDGAHDLVAALNKAVHLVTG
                     LEQRLGSLLFKELLALHFSPTRPTKDEWTDHPVIVLLVAEIERARCDGEVHPAVDAFY
                     SAAFFLLGVYGTLTTTGNSDTRDTMLAKLVDTAVRGLEIR"
     gene            5762..7294
                     /locus_tag="VGP27_12215"
     CDS             5762..7294
                     /locus_tag="VGP27_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7852100.1"
                     /translation="MSELSESHALAVERSCAVTAVALSGQRREGVRLVSGQRRDFGLS
                     STLDFIHVPYPPPVRDWTRRTFTCGVALQCSPSKERILDYRLNELSVRELSALTLVEA
                     GVALGWIAENWPGLLTELRRLLPDLDVTTGGTGAKEMLNRAVALARTSQPLLVDPLLG
                     TLPRAYTDPQGLTDKLRRSFGRMPWTTSQKRLPRAHSVPVGGDGGVRNPNLPPPSRPR
                     DNDLDVTPDHRPGIPYPEWNTWTKSFMADHVAVLERAHTSHQRHPVTTSADLRKWFSE
                     HTHRSMRNRLEDGSDLDVDQYVSHYIDLATGEAVEPRIFRDLLPSSRDVTTALLLDGS
                     SSLGVHGGRIFKLELACADALSQAMALARERHGIFVFTGNTRHRVEVNCFKDFEDRRF
                     APPSGLGLSTGGYTRLGAPLRHLTSRLLAQPSERRLLIVIGDGLISDEGYEGRYAWAD
                     AAHAVEEANDAGVSIYYVGVGPTRVDPLPEVFGPRRSQRIRRVEDLPRVLAHVHRELV
                     AA"
     gene            7291..8091
                     /locus_tag="VGP27_12220"
     CDS             7291..8091
                     /locus_tag="VGP27_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEV7852101.1"
                     /translation="MTDTYFANGNEVQLFEQAYRQRLPVMLTGPTGCGKTRLVEHMGV
                     LLRRPVVTVSCHDDLTSSDLVGRFMVTGGDVVWTDGPLTRAVKAGAICYLDEVVEARH
                     DSLAILHSLTDHRRALYLDRADEVVDAPEPFMLVCSYNPAYRSSLKELKPSFRQRFVT
                     LPMRYLPPDREAEVIVAEAAIDSAVANRLVQCATAIRTADEAFHFEPPSTRVLVTAAQ
                     LIAAGASELEAAEACILAPLSSDGAISDGLREVAAASLAAADSSSRSL"
     gene            8109..8237
                     /locus_tag="VGP27_12225"
     CDS             8109..8237
                     /locus_tag="VGP27_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852102.1"
                     /translation="MDQKEKKRKRALIVLQIVIYGYLLTMFLIQLYMSFARGWWEL"
     gene            8249..8959
                     /locus_tag="VGP27_12230"
     CDS             8249..8959
                     /locus_tag="VGP27_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852103.1"
                     /translation="MNRRSGAKARPLGGSVSLDEHDEESRLAQRRADKWMIVGAALMG
                     MWAPGLIGFPIFMRGVWLQRQAARAGLSMRPMIVTLIGYLVMIDGMLNSLGWALDLVA
                     NHTLINRVLMVGWGAMFDAGYFWHYNELWVGGAAGPGEKSMVFGMILTVFAMRCAAAI
                     GFLQMKRWGQQWMIITCWMGVLIWCLYVFNMTIYADVRFSGVVFPVIGWWLYDIFYIT
                     PFLAIPYLHTVNREIFSD"
     gene            8981..9421
                     /locus_tag="VGP27_12235"
     CDS             8981..9421
                     /locus_tag="VGP27_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852104.1"
                     /translation="MSSPTSTPESAEDQKAEDLPPGTTPYYARMHKYIKRAVLVCLVA
                     LVVEGAFTLPFMAVYYGYPTLSLTEICSELLKVRYSNDTLECKVPYPPFGPPEGAEGK
                     DTAQDEWGIQPVPKYNRIGWRELVRIHDEREARQAAEQQGAPAP"
     gene            9418..10638
                     /locus_tag="VGP27_12240"
     CDS             9418..10638
                     /locus_tag="VGP27_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852105.1"
                     /translation="MTKSAEEHAQNWDLRHEDFKDNDFLYDVYSVMRSTAPFAHTDTP
                     FLSATPGGAWVATRYEECYRILQDWEHFSSNPTPEASERLAGDLVITLDPPRQQKFRK
                     VLNPYFSPARMKALRPQIQAETDQIIDAFIADGSGDLADVAWRQPGIVFFKYLLGMPI
                     DDVPLCIERTDTALNGGTEESRMAAWGALYQHINDAVTARTTQPPRDDMIDVLLNAEI
                     DGEKLAFGDIVSNAMLLVQAGLETTASAMSYAFHYLAIHPAERDRLIREPELLPRAVE
                     EFIRFAGSIHGIPRTVAREVELNGHSFCPGESVVVNYASANRDAGQFPEPDRCILDRR
                     ENRHLGFGAGVHRCLGSNLARLEFHIGLEQVLSRMPDFTLAKSTEAIFHGNSVTRGFR
                     RIPIAFTPNGRSAV"
     gene            10635..11651
                     /locus_tag="VGP27_12245"
                     /pseudo
     CDS             10635..11651
                     /locus_tag="VGP27_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956409.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
     gene            complement(12235..13554)
                     /locus_tag="VGP27_12250"
     CDS             complement(12235..13554)
                     /locus_tag="VGP27_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852106.1"
                     /translation="MTVHDDQVSHRQSTPSDIRKAVRGAAIGNTVEWFDFAVYSVLAT
                     YIADKFFPSGDDTVALLNTFAIFAAAFFMRPLGGFFFGPLADRIGRQRVLAIVILLMS
                     ASTFAIGLVPSYDSIGVGAPILLLLLRCLQGFSAGGEYGSGACFLAEYAPDKHRGFVV
                     SFLVWSVVLGFLLGSITVTVLQAVLPEAAMESYGWRIPFLLAGVLGAVGLYIRLRLRD
                     TPEFEELRDEGEISESPLKEALTTSWRPILQIFGLVVIHNVGFYTVFTFLPSYFTKTL
                     EFTKMDAFVSITVASLVALILIPPLGALSDRVGRKPLLIAGAIGFIVLTYPLFLLLNT
                     GSLVGAIAGHAGLAAVEAVFASTALAAAAELFATRVRSGAYSIGYNISVAIFGGTAPY
                     VATWLTDRTGNPLSPAYYVILAAVVSLATVLTMRETAARPLRKLATH"
     gene            complement(13551..>14255)
                     /locus_tag="VGP27_12255"
     CDS             complement(13551..>14255)
                     /locus_tag="VGP27_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEV7852107.1"
                     /translation="QANLVILPACHALDFLRFCVRNPKPCPLIEVTDTGSPHPDTFSR
                     DADLRTDVPRYRVFENGSLVDEPTDVSRWWRDDLVSFLLGCSFTFEWALAAAGLPLAH
                     QRQGINVPMYVTDRRCAGAGPFEGPLVVSMRPFAPEDVARATEISARFPAMHGGPVHA
                     GDPATLGITDLAAPDFGDPVAIEPGEVPVFWACGVTPQAVVLEAKPSLAIFHAPGHMF
                     ITDRPHAEFDSQEKSS"
BASE COUNT         2692 a         4496 c         4456 g         2611 t
ORIGIN      
        1 ggaccgaacg aatcgaaaac agtgcagttg ctgcgcgcga tggtcgcgaa cgtcgtcttg
       61 tgcacggtcg cgaagtggta gttctccgcg aaggtgtcga tcgcgagctt ccagttgatc
      121 gccgtgtcca gcaccttctc gccaagcggc gcccagcgtc cgatacccca ggagtccaat
      181 tcgtcggcca gcggtccgag gaactcgcgg atgtccaggg tcgcgtgcgg atcgagcgca
      241 acccagagga atcccgcgca ttcggttgcc ggcagctcgc tcagtttcgc actgcccgtt
      301 gagctctcgg gaaagccctc cttgccgggg atgccgacca ggttgccgtc tagatcgtag
      361 ttccacgcgt ggtaaggaca cacgagacgg cgcgccgacc cgcagccttc ggtgatctgt
      421 gactgccggt gcaggcagac attctcgaac gcctttaccg tcccgtccga cgtgcgggtc
      481 agcaggatgg accggcccat cacggtcttc gtacagtagg agccgggctt cggcagttcc
      541 gagacgtagc cgaccagctg tgggctggtg agcaccatct ggacgtccct cgcgtgacgg
      601 tcggctgagg tgtacgtatc ggcggccacc ctgcattcgg caggagccag gtcggtcgtc
      661 ttgtcccgtg ccagtttgag tgcacgccgg gtgaggtcga cgagctgatc acgatccatg
      721 gcggctatat tactattttt ataaagattg gtgaaggccg gagcgcctcg agccgcgtca
      781 tcagctagtt gatcgtcaca ccggcatcga ccttgaactg cagtccggtg acgaatcgtg
      841 actcgtcgga tatcaggaac agcaccgcat tgctgacatc aaccggttcg acgcatgggg
      901 tcggcatcgc gttgatgaag agcggcacca ggtccgaccg ttcaccgccg agggcgacgc
      961 cgagggaaga cggaagcatc cccgtgggca ccccggtggg atgcacggag ttgacgcgga
     1021 cactctgtgc agcaagctcg ttggccaatg acaaggtcag cccgatgacg ccgtgcttgg
     1081 ccgccgtata gggcacctgg agtggaaatc ccgcgattgc gcctgccgag ctgatgttca
     1141 ccaagctgcc gccaccctgc tcgaggagat gcggaatcga agccgcgcat gtgttccaca
     1201 ccccgatgag gttgacgtcg accaccgtgc gccagtcctc ggaggtcgtc tgatgccagg
     1261 ggccgcctgt cagcacaccg gcgttggcga ccgcgccatc caacccgccg agctcggcga
     1321 cgccatcatc gaccgctgag cgcagcgccg cctcgtcgcg gacatcgacg acgtggctta
     1381 cgcatcggcg accgaggtcg gagaccagcc ggaccgtctc ggccaagtcg tcctcggtcg
     1441 ccaagggata ttcgatctcg gggagggacc ggcacaagtc gaccagtacg aggtctgcgc
     1501 cctcctcggc cagtctcacg gcgtggctgc gccccaggcc tcgggcggcg cccgtgatca
     1561 gcactcgctt gcctgcgacc cgtcctgtcg cggacgcgct caccacatca agccacgaat
     1621 gatctccgcc tgcggaatgt cctccacagc gatcagtcct tggggagccc ggcagatcca
     1681 gtcgatcgcg ttcacgaccc gcgccgccgt cgacagacac cccgcctcgg tgacgtcgag
     1741 ccccgggtgg gccacgtgtg tgttgagttc gacgcgcgga tctccttcga ccaggactcg
     1801 atgcactccc gggtggccgt cgggcgggta ggcccattgc ggtgccgacg cgtcggtgag
     1861 tcgcgtgatg tgttccatgg ttatcaccgg aaccccatcg cgcaccccct cgaccgcgaa
     1921 cttgaccgcg gccatttctc ccggttccac cgtcgccatc ttgcaaacga ttttgttgtc
     1981 ggtgtaccac ggctcgatgc gttgacggag ctcgtcgagt tgcactccaa gctggtcggc
     2041 gaggttgcgt acggctccgc cccacatcgt ggtcaggaca cccggcagaa agagcatcgg
     2101 cgtcgcttca tcggcgctga cgccgaaacc cattgacttg ccggtgaact cgaagtcgtc
     2161 atagttggcg tagtcgaaga tctcctgaac ggtgatcgac cggacacggg tggtcaggct
     2221 cagcgctgag tgcacctcgg tgtcaccgga atagccggga tcgatcccgt tgacatacag
     2281 cgaggcgttg cccgcctcgc aggcttgttc caagggcacg cgcagccagt cgtcaacctg
     2341 gcgaggggtc accagccaca ccatcgacgt cgctgcgata ttgatgccgg cggacaagaa
     2401 cttggacatc tgcacgatca cttccatcgg ccgtgtctca ccctgcgcgg tgtagaccac
     2461 gcaatccggc ttcagtgcaa ttagcgcgtc gatgtcatcg gtcgcgatga tgccggtcgg
     2521 ctcggtcaaa ccgcacagtt cggcggcatc acgcccgatc ttcttcggac tggctgcatg
     2581 cacaccgacc aattcgagat cggggcgtcc gatgatcgcc ttcagcgagt ggactccgac
     2641 gtttccggtg gagaactgaa ctactctgcg catcttcagg tcgtccttgc tcagtagtcg
     2701 gggatcggca gcggcgaatt gttggagtcg atgccgccgt cgacatggaa tatcgcattg
     2761 gtcgcatagc agctgggcgt ggagagataa acacagagcc gcccgaggtc ctcgacgtcg
     2821 ccgagccggt gcaggggcgt tgactcctgc atcttctcca acgatcccgg catcatatcg
     2881 aggctgccct gaagaccatc ggtcgcgaac gagccgagag cgatcgcgtt gacgcggatc
     2941 ttcggcgcga gctcctgcgc catggctcgt gtcagggcct cgagtccccc cttggccacg
     3001 caataggcgg tcagcgcgcg gatgccgaac cgggctgagc ccgaggagat attgacgatg
     3061 ttcccgtggc cggattcgat catgtggaag ccacgagctg gctcatgatg aacgccgagg
     3121 tgacgcacca gtcgaacgtg tggcggaagt cgtcatcggt gatgtcgagg aaacgcgcgt
     3181 aggtcgagcc gccgacgttg ttgatcagaa tgtcgatgcg cccgaaccgt tccatggccg
     3241 tcgagacgac gcgttctccg tccggacgac tcatcgcatc cgcgacgagg gcgagtccct
     3301 tgccaccggc ctcttcgaca ccggcgatcg tcccggcgat gtcagattct gtacgggcgg
     3361 tgccgacgac ggttgcccct gcctccgcca ggactctcgc aataccctga ccgacgccct
     3421 tcccggcgcc cgtcacgatc gcgacctgtc cgtccagatt gaattgctcc agcgccattc
     3481 cggtctttcc atcgagtgct gatcatctaa cgacgttgat tgactgaagt caacaaagtg
     3541 gccccggcac cagacagctt ttagacggta gagtcgctgt atggcagtgg tagacaagcc
     3601 gtccgcgcga gagaccaaac gtctgcagac ccgcgagcgg ctgatgggag cggcgatcgc
     3661 agagttcaaa cgcggaggga tggccgaggc cgacgtcggt gcgatcgtcg ccgcggcggg
     3721 cgtcgcccac ggcacattct tcttccactt ccccaccaag gagcacgtac ttctggaatt
     3781 ggagcagcgt gaagaggagc ggatcgccaa gcagctggcc cgctccacgg ggccggaaag
     3841 cgatctagtc tcgacgctca acgaaacggt ccgcctggtg atggggctgg aacggcgact
     3901 tggtgccgtg ctattcaaag acttcctggc actgcacttt tcacagaccc ggccctcgtt
     3961 ggacgagagt cgccagcatc cggtgatcgt gaaggtagcc caggagatcg agctggctca
     4021 gaagcggggc gccgtcgacc ccgatgtgac cccgatgaac agtgcggtgt tctttttgct
     4081 cggtctctac gcgttgttga cgactacaca cgactggccg gggcaggccg cgatgatcga
     4141 ggattatgta gcgaggacac ttcgcggcat tgctgtgaag taggtaattg actactggaa
     4201 ctggttgact atagtcagtc ttcatgaatc tgcccacggt cgatctgggc cgtaatcgcg
     4261 acgctgtgca gcgggcgaaa ctctcgggaa gcggcctgga cgagcacatg gcggcgtcgc
     4321 aggcggcgca gcgccaggcc gacaagtggt tgatctcggg cagcatcctc atcggcaccg
     4381 ccgccctcgg catcttcggt ttgccgcttt tcctccgggg tgtgtggttg ttacgcaagg
     4441 cgcagcgcga tggcctgacc gtccggccga tgctggtgac gctgatcggc tacctcgtca
     4501 tcatcgatgc cgccatcaac accgtgggat gggcgctcga cctggtggcc aaccacacga
     4561 ttcttgcccg aatcctgtta agcggctggg gagcgatgtt cgatgccggc tacttctggc
     4621 attacaacga actgtggctg ggcggcgcag caggcccggg cgagaaagct atggaagtcg
     4681 gaatgatcct caccgtcttc acgatgcgga tcgccgcggc gatcgggttc ctgcagatga
     4741 agcgttgggg ccagcagtgg atgatcatca cctgctggat gggcgtactc atctggtgcc
     4801 tgtacgtgtt caacatgacc atgtacgccg acgtccgctt cgccggtgtc gtcctgcccg
     4861 tggtcggctg gtggctgtac gacatcttct acatcactcc gtttttggcg atcccgtacc
     4921 tgcattcggt caaccgcgaa atcctctccg actgaagtca atgaccaaac tcattgacta
     4981 tggtcagtaa ctaagttaca aagggcggac catgacgcct caggtcaagc aatcggcgcg
     5041 ccaactccgc cgcctgcaga cgcgggaacg catactcggc gcagccatcg ccgagttcaa
     5101 gcttgcgggc atgtccggcg ccgacgtgaa cgcgatcgcg acggctgcag gcgtggcaca
     5161 cggaaccttc ttctttcact tccccagcaa ggagcacgtg ctgctcgagt tggagagtcg
     5221 cgaggaaggc cgtatggcgg ccgaattctc ccacttcctc gacggtgcac atgatctcgt
     5281 ggcggcactg aacaaggccg tgcacctggt gaccggcttg gagcaacgac tcggttcgct
     5341 gctattcaaa gaattactcg cattgcactt ctcaccgacc cggccgacca aggacgagtg
     5401 gaccgaccat ccggtgatcg tgctgctggt tgccgagatc gagcgggcac gctgcgacgg
     5461 cgaagtacat ccggcggtcg acgcgtttta cagcgccgcc ttcttccttc tcggtgtcta
     5521 cggaacgctc accaccactg gaaactccga tacccgcgac acgatgctgg ccaagttggt
     5581 cgataccgcc gttcgcggtc tggagatccg atgaatacat ccatcggcaa ggaggcctga
     5641 catggattgg atttgggaga ttctgcgata cgtcgcagct tggggcggaa cgggtttgat
     5701 catctggttc tggtattgga tgttctccaa catcggcacg ttctgagcga cccgactcga
     5761 tatgtctgag ctctccgaat cacacgcgct cgccgtcgag cgcagttgtg ccgtcacggc
     5821 cgtcgccctc agcgggcagc gtcgtgaggg tgtgcgcctg gtgagcggcc agcggcgaga
     5881 ttttggtttg agctcgaccc tcgacttcat ccacgttccg tatccgccac cggtacggga
     5941 ttggacgcgc aggacgttca cgtgcggcgt agcgcttcaa tgttcaccat cgaaagaacg
     6001 aatcctcgac taccgattga acgaactgtc ggtgcgagag ttgagcgcgc tcaccctcgt
     6061 ggaggccggc gtcgcacttg gatggatcgc ggagaactgg cccgggctgc ttaccgaact
     6121 gcggcggttg ctgcccgacc tcgacgtgac cacgggcggg acgggcgcca aggagatgct
     6181 caatcgcgca gtcgcactgg cgcgtacgtc gcaaccgcta ctcgtcgatc cactgctcgg
     6241 taccctgccg cgggcctaca ccgatccgca gggactcacc gacaaattgc gtcggagctt
     6301 cggcaggatg ccgtggacca cgtcgcagaa gcgacttcca cgggcgcact cggttcctgt
     6361 cggcggagac ggtggcgtac gcaaccccaa cctgccgccg ccgagtcggc cgcgggacaa
     6421 cgacctcgac gtcactccgg accatcgtcc cggcatcccg tatcccgagt ggaacacgtg
     6481 gacgaagagc ttcatggccg accacgtcgc agttctcgag cgcgcgcata catcccacca
     6541 acgtcatccg gtgactacgt cggccgatct gcggaagtgg ttctccgagc acacccatcg
     6601 gtcaatgagg aaccggttgg aggacggctc agacctggac gtcgaccagt acgtcagcca
     6661 ctacatcgac ctggcgaccg gcgaggcggt cgagccgcgc atcttccgcg atctgctgcc
     6721 cagcagccgc gacgtcacca cggcgctgct tctcgacgga agctcgtcgt tgggagtgca
     6781 cggcggcaga atcttcaagc tggaattggc atgtgccgat gcgctttccc aggcgatggc
     6841 gcttgcccgc gaacggcacg gcatatttgt gttcaccggc aacacccggc atcgcgtcga
     6901 ggtcaactgc ttcaaggact ttgaagaccg ccgattcgcg ccaccgagcg gcctgggtct
     6961 gtcaaccggt ggttacaccc ggctcggcgc accccttcgc catctcacaa gtcggctgct
     7021 cgcccagccg tccgagcggc gcctgctcat cgtcatcggt gacggcctga tctccgatga
     7081 gggctacgag ggccgttacg cgtgggccga cgccgcgcac gccgtcgagg aggccaatga
     7141 cgcaggcgtc tccatctact acgtcggagt gggcccgacc cgggtcgacc cacttccgga
     7201 ggtcttcgga cctcgccggt cccaacgcat ccggcgtgtc gaggatcttc cacgggtgct
     7261 cgcccatgtg catagagagt tggtcgccgc atgaccgata cgtacttcgc caacggtaac
     7321 gaagtccagt tgttcgagca ggcttaccgc cagcgcctcc cggtgatgct caccggccct
     7381 acgggctgcg gcaagacccg actggtcgag cacatggggg tgctgttgcg tcggcccgtg
     7441 gtcaccgtca gctgccacga cgacctgacc agctcggatc tcgtcggcag gttcatggtg
     7501 accggtggcg acgtggtgtg gaccgacggt ccactgacca gggcggtcaa ggccggggcc
     7561 atctgctatc tggacgaggt cgtcgaagca cgacacgatt cgctggccat cctgcactca
     7621 ctgaccgatc accgccgcgc gctgtatctg gaccgggccg acgaggtcgt cgatgcaccg
     7681 gaaccgttca tgctggtgtg ctcctacaac ccggcctatc gcagctcact gaaggaactg
     7741 aagccgtcct tccgtcagcg cttcgtcacg ctgccgatgc ggtacctgcc tcccgatcgg
     7801 gaagccgaag taatcgtggc cgaggcggcc atcgactccg cggtggccaa ccggctggtg
     7861 cagtgcgcga cggcgatccg caccgccgac gaggccttcc attttgagcc gccgtccacg
     7921 cgggtgctgg tcaccgcggc gcagttgatc gcagcgggcg ccagcgaatt ggaggccgcc
     7981 gaggcctgca tcctcgcacc actgtcgagc gacggtgcga tctccgacgg tttacgcgaa
     8041 gtcgcggccg ccagtctggc tgccgccgac agctcgagca gatcactcta ggaaggagcc
     8101 gcggcgacgt ggatcaaaaa gaaaagaagc gcaagagagc tctcatcgtg ctgcagatcg
     8161 tcatctacgg ctacctgctg acgatgtttc tcattcagct ctacatgtcc ttcgcccggg
     8221 ggtggtggga gttgtgaatc tgccactact gaaccgtcgt tcgggcgcaa aggctcggcc
     8281 tctcggtggc tcggtgagtc tcgacgagca tgacgaggaa tcgcgcctgg ctcagcgccg
     8341 cgccgacaag tggatgatcg tcggcgccgc gctgatgggc atgtgggccc cgggcctcat
     8401 cggattcccc atcttcatgc gcggggtttg gctgcagcgg caggccgcgc gcgccggtct
     8461 gtcgatgagg ccgatgatcg tcactctgat cggatacctc gtcatgatcg acggaatgct
     8521 caacagcctc ggctgggcac tcgacctcgt tgccaaccac accctgatca accgcgtgct
     8581 gatggtcggc tggggcgcca tgttcgacgc cggctacttc tggcactaca acgaactgtg
     8641 ggttgggggc gcagccgggc cgggcgagaa gtccatggtg ttcgggatga tcctcaccgt
     8701 gttcgcgatg cggtgcgccg cggcgatcgg cttcctgcag atgaagcgct ggggccaaca
     8761 gtggatgatc atcacctgct ggatgggcgt gctcatctgg tgcctctatg tgttcaacat
     8821 gaccatctac gccgacgtgc gattctccgg cgtcgtcttt ccggtcatcg gctggtggct
     8881 ctacgacatc ttctacatca caccgttcct ggcgatcccg tatctgcaca cggtgaatcg
     8941 cgaaatcttc tccgactgaa ctgattcgag gagcattgca gtgagttcac caacttcgac
     9001 acccgaatcc gccgaggacc agaaggccga ggacctgcca ccgggcacca cgccgtacta
     9061 cgcgcggatg cacaagtaca tcaaacgcgc agtgctggtc tgcctggtcg cgctcgtcgt
     9121 cgagggcgcc tttacgttgc cgttcatggc tgtgtactac ggctatccga cgctgagcct
     9181 gaccgagatc tgcagcgaac tgctgaaggt ccgatactcc aacgacaccc tggaatgcaa
     9241 ggttccctac cccccctttg gccccccgga aggggctgaa ggcaaggaca ccgcccagga
     9301 cgaatggggc attcagcctg tcccgaagta caaccggatc ggttggcgcg aactggtgcg
     9361 cattcacgac gagcgggaag cccgtcaggc cgccgagcag caaggtgctc cggctccgtg
     9421 accaaatcgg ccgaggaaca tgcgcagaac tgggaccttc gacacgagga cttcaaggac
     9481 aacgacttcc tctacgacgt ctattcggtg atgcgcagca ccgcgccgtt cgcccacacc
     9541 gacacccctt ttctcagcgc cactccaggt ggcgcgtggg tggcgacccg atacgaggag
     9601 tgctatcgga tcctgcagga ctgggagcac ttctccagta atccgacgcc ggaagcgtcc
     9661 gagcggctcg caggcgacct cgtgatcacg ctggatccgc cgcggcagca gaagttccgc
     9721 aaggttctca acccgtattt ctcgcctgcc cgaatgaagg ccctgcggcc gcagatccag
     9781 gcggagaccg accagatcat cgacgctttc atcgccgacg gcagcggcga tcttgccgat
     9841 gtcgcttggc ggcagcccgg catcgtcttc ttcaaatacc tgctcggcat gccgatcgac
     9901 gatgtgcctc tgtgcatcga gcgcacagat acagcactca acggcggcac cgaggaatcc
     9961 cgaatggccg cgtggggcgc gttgtatcag cacatcaacg atgcggtgac ggcccgcacc
    10021 acgcagccgc cccgtgacga catgatcgac gttctcctga atgccgagat cgacggcgag
    10081 aagctggcat tcggcgacat cgtctccaat gcgatgctgt tggtgcaggc cggtctggag
    10141 accaccgcga gtgcaatgtc ctatgcgttt cactatctgg caattcaccc ggcggaacgg
    10201 gatcggctca tccgcgagcc tgaattacta ccacgagccg tcgaagagtt cattcgcttc
    10261 gccgggtcga tacacggaat tccccgcact gtcgcgaggg aagtcgaact caacgggcac
    10321 tccttctgcc caggggagtc cgtagtggtc aactacgcct ccgccaaccg cgacgccggc
    10381 caattccctg agccggatcg ctgcatcctc gatcgccgcg agaaccggca cctcggattc
    10441 ggtgcaggag tccatcgatg cttgggttcc aatctggcgc gcctcgagtt ccacatcggt
    10501 ctcgaacaag tcttgtcgag aatgcccgac ttcaccctcg cgaaaagcac agaagcgata
    10561 tttcacggga actccgtcac cagaggcttc cgccggatcc cgattgcttt cacccccaac
    10621 gggaggtcag cggtatgacc tatcgagtcg tccagtggac gaccggcaac atcggtacga
    10681 agtcggttca tgcagtcgtc gagaattctc agctcgagtt ggtcggatgt tacgcgtggt
    10741 catcggagaa agtcggccgc gatgtcggag aactctgcgg catcgaacca ttcggtgtgc
    10801 tggcaaccga tgacgtcgat gccctgcttg ccttgaagcc ggattgcgtt gtctacaacc
    10861 agatgttcgc cgacgtcgac gatcttgtcc gcattctcga ggcgggcgtc aatgtcgtaa
    10921 caacatcgga gttcatcacc gggcaccggc tcggcgacgg ccgcaaacgc atagcgcggg
    10981 catgcgagct cggcggatcg acgatcttcg gcagtggcct gaatcccggc ttcatccagc
    11041 tcttcgcgat cgtttcagca gggctgtcgg atcgggtgga ccgggtatcg atcgtggaga
    11101 tgttcgacac cacgatctac aactccccgg agaccgagaa gatcatggga ttcgggtatc
    11161 ccatcgacca tccagaactg cttgcggtga ccgaggaggg atcggggata tttcgcgaag
    11221 ccgtcctcat cgtcgcccat gccctggggg tggatctcga cgatatccgc tgcgaggccg
    11281 cctacgccca agccaccgaa gatatccagc ttccgggcga ctgggtaatc gcccaaggct
    11341 gtgtggcggg aattgatgtg agctggaagg gcttcgtcgg tgagcgggac gttgtcgagg
    11401 tgcgcggagt ctggaccaaa ggacaagcgc tggaaccaac ttggacgatg ggtttcggat
    11461 acaacatcac ggtctagggg aggccgacta tcaagaccac cctagccttc gagccgccac
    11521 cggacttcgt cacagagacg atcgaggact acatcctgct cggtctgacc atcaccgcgg
    11581 tgcctgcgat caccgcgata cccgccgtcg tcgcggcgcc acccggcatc gcgacctaca
    11641 acgatctacc ggagggcgac caacatcgcc gtctgcgaag cctggtgtca agggccttca
    11701 ccccgcgcgc gaccgcgcgc atgcacgaca cgatccacac ggtggtcaac gagctgatcg
    11761 atcgagtggc cgcccgaatg ctcgatctgc ccaccacgat cgcgcgcatg aacgtcggtt
    11821 acagttggac cgggcacggc gggttaccag tgatgatgta tcacacgatg aaagcagggc
    11881 agccctggcc cgtgcctatc ggatattaca aagtaggctc gccgatcggc ggcgttgaca
    11941 tcggtatgca agcctacaag cttctcgaaa tcgcctctgt cccagcaacg atcgtaaatt
    12001 ggggtggcga tgacgccgta tcggatcgtg agatgggtga gtacgtcgcc gacctgacag
    12061 acatcaccgt gaacctcgcc gagagcccga tcagctttga ctccttcccg tccgacaacc
    12121 gtcgccgtga acagctcatc ggcaagtgct cggtgcactg gaagaacggc gttcgagaca
    12181 ctttcgagcg cctcggactg ctcggaagct agttccgcgc accggtatga agcgctagtg
    12241 cgtggcgagt ttgcgcaacg gtcgcgccgc tgtctctcgc atagtcagaa ccgttgcgag
    12301 ggatacgacg gctgcgagga tgacgtagta cgcgggcgac agcgggttac cggtgcggtc
    12361 ggtgagccag gtcgccacgt acggcgctgt gccaccgaag attgcgacag aaatgttgta
    12421 gccgatcgag taggcgcccg atcgcacccg cgttgcgaac aattcggcgg cggcggccaa
    12481 tgccgtcgac gcgaacaccg cctcgacggc ggccaagccg gcgtgcccgg cgatcgcccc
    12541 caccaaagat cccgtattca gaagcaggaa aaggggatac gtgagcacga taaatccgat
    12601 cgcacccgcg atcaacagcg gcttgcgtcc gaccctgtcg gacagggcgc cgagcggcgg
    12661 aatgaggatc agcgcgacca ggctggcgac ggtgatcgag acgaaggcgt ccatcttggt
    12721 gaattcgagc gtcttggtga agtagctcgg caagaacgtg aacaccgtgt agaagccgac
    12781 gttgtggatg accacaagcc cgaagatctg gaggatgggg cgccacgacg ttgtgagtgc
    12841 ctccttgagc ggcgactccg atatttcgcc ctcgtcgcgc aattcctcga actccggcgt
    12901 atcccgcagc cgcagccgga tgtagagccc gacggcgccg agcacgcccg ccagcaggaa
    12961 tggaatccgc catccgtagg actccatggc cgcttcggga agcaccgcct gaagcacggt
    13021 gaccgtgatc gatcccagca ggaatccgag gacgaccgac cacaccagga acgagacgac
    13081 gaacccgcga tgcttgtccg gcgcgtactc agcgaggaag cacgcgccac tgccgtactc
    13141 gccgcctgcc gaaaagccct gcaagcaacg gagaagaagc agcagtatcg gggcgccaac
    13201 gccgatcgag tcgtagctcg gtaccagacc gatcgcgaac gtcgacgccg acatcaacag
    13261 gatcacgatc gccagcacgc gctggcggcc gatccggtcc gccaaggggc cgaagaagaa
    13321 cccgccgagt ggtcgcatga agaaggcggc cgcgaagatc gcgaaggtgt tgagtagcgc
    13381 caccgtgtcg tcgccggacg ggaagaactt gtcggcgatg taggtggcca gaacactgta
    13441 gaccgcgaag tcgaaccatt cgacggtgtt gccgatcgcc gccccccgca ccgcttttcg
    13501 gatatccgac ggtgtggact gccgatgcga gacttggtcg tcgtgcacag tcatgatgac
    13561 ttctcctgag agtcgaattc ggcgtgcggt cggtcggtga tgaacatgtg gcccggcgcg
    13621 tgaaagatcg ccagcgatgg tttggcctcg agcacgaccg cctgcggtgt cactccgcac
    13681 gcccagaaca ccggcacttc acccggttcg atcgcgaccg ggtcaccgaa gtcgggtgcg
    13741 gccagatcgg tgatgcccag tgtggcgggg tcgcctgcat ggaccggccc gccgtgcatc
    13801 gccgggaaac gggcggagat ctcggtcgcc cgcgcgacat cctccggcgc aaacggtctc
    13861 atcgacacca ccagcggacc ctcgaacggt cctgcgcctg cgcagcggcg gtcggtgacg
    13921 tacatcggaa cgttgatgcc ctggcgctga tgcgccaacg gcagaccggc cgcggcgagc
    13981 gcccattcga acgtgaagga gcaacccagc aagaagctca ccagatcgtc gcgccaccac
    14041 ctgctcacgt ccgtcggctc gtcgaccagt gagccgttct cgaatacgcg gtagcgcggg
    14101 acatcggtgc gcagatcagc gtcccgcgag aaggtgtcgg gatgtggcga gccagtgtcg
    14161 gtgacctcga tgagcggaca cggcttcggg ttgcgcacgc agaagcgaag gaagtcgagc
    14221 gcatggcacg cgggcagaat cacgaggttg gcctg
//
LOCUS       DASYLX010000507         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3425946,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000507 DASYLX010000000
VERSION     DASYLX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425946"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VGP27_12260"
     CDS             <1..610
                     /locus_tag="VGP27_12260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7852108.1"
                     /translation="SLTGRAAIWLVDADILHENFEPLTGMFNRDGFYEKATTLLASRS
                     RGDDRFLAVAVVNLDSFSLVGEFSGASGSNRARVEIAARLRETARRDAVLAHIADSEF
                     VIADLFESNDASALIERVRGTISGSPSRLSASIGVVTTALQPLVQLPPHDVLDELISI
                     ATNAMYVSRKSGGNQVTYVRGPSLTVLDDPGHDDWFDTEEPA"
     gene            complement(621..1217)
                     /gene="pdxT"
                     /locus_tag="VGP27_12265"
     CDS             complement(621..1217)
                     /gene="pdxT"
                     /locus_tag="VGP27_12265"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV7852109.1"
                     /translation="MSGPRVGVLALQGDTREHLAALREAGAEAMTVRRRSELDAVDGL
                     VIPGGESTTMTHLLREFQLLEPLRARLAEGMPAYGSCAGMILLASKILDAGVAGRDAA
                     PLNGIDMTVRRNAFGRQVDSFEGDIDFDGLDRPVHAVFIRAPWVERVGPDVRVLGRAA
                     DHIVAVRQDRMLATSFHPEMTGDRRVHQLFVDIVTGRA"
     gene            complement(1214..>1762)
                     /locus_tag="VGP27_12270"
     CDS             complement(1214..>1762)
                     /locus_tag="VGP27_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEV7852110.1"
                     /translation="SMSASFQTDQSGIEHQDAAPFAVPPDDLPNFISKGGVFDEAGFQ
                     QFAEWDVRMVPRDELKLLPGKASQQQVWFRHRDPLPDDPVLHICALAYMSDLTLLGSA
                     QVTHLEERKHLMVASLDHAMWFMRPFRADEWLLYDQSSPSASGGRSLTQGKIFTQSGE
                     MVAAVMQEGLTRYKRDFTSARQ"
BASE COUNT          298 a          618 c          565 g          281 t
ORIGIN      
        1 ctcgctcacc ggccgggccg cgatctggct cgtcgacgcc gacatcctgc acgagaactt
       61 cgagccactc acagggatgt tcaaccgcga cggcttctac gaaaaggcca ccacgctgct
      121 ggcatcgcgc agccgcggcg atgaccggtt cctcgccgtc gctgtggtca acctcgacag
      181 cttttccctg gtgggggaat tctccggggc gagcggatcc aaccgcgccc gcgtcgagat
      241 cgccgcccgc ctgcgggaga ccgcgcgacg cgacgcggtg ctcgcgcaca tcgccgactc
      301 cgagttcgtc atcgccgacc tgttcgaaag caacgacgcg tcggcgctga tcgagcgggt
      361 tcgcggcacg atctcgggct ccccctcccg cctgtcggcc agcatcggtg tggtgacgac
      421 cgctttgcag ccactcgtgc agctgcctcc gcacgacgtg ctcgacgaac tcatatcgat
      481 cgccaccaac gcgatgtacg tatcacgcaa gagcggcggg aatcaggtca cctacgttcg
      541 cggtccgtcg ctcaccgtgc tcgacgatcc cgggcacgac gattggttcg acaccgagga
      601 gccggcctag cgctcacccg tcacgcgcgg ccggtgacga tatcgacgaa gagctggtgg
      661 acgcggcggt cgccggtcat ctcggggtga aacgacgtcg ccagcatcct gtcctggcgg
      721 accgcgacga tgtggtcggc ggctcggccg agtacccgca cgtcaggacc gactcgttcc
      781 acccacggtg cccggatgaa caccgcgtgc accggtcggt caaggccgtc gaaatcgatg
      841 tcgccctcga aggagtcgac ctgccggcca aaggcattcc gccgcacggt catatcgatc
      901 ccgttaagcg gagcggcatc gcgtcccgca acgcctgcgt ccaggatttt gctggccagc
      961 aggatcattc cggcgcagga accgtaggcg ggcatgccct cggcgagccg ggctcgcagc
     1021 ggctcgagta gttggaactc gcggagcaga tgcgtcatcg tggtggactc cccgccgggg
     1081 atgacgagcc cgtcgacggc gtcgagttcg ctgcggcgcc ggaccgtcat ggcctcggca
     1141 cccgcttcgc gcagcgctgc caaatgctcg cgggtgtcac cctgcagcgc cagcacgccg
     1201 acgcggggcc cgctcactgc cgtgccgagg tgaaatcgcg cttgtagcgg gtcaacccct
     1261 cctgcatcac cgccgccacc atctcgccgg actgggtgaa aatcttgccc tgcgtcaacg
     1321 accggccacc ggaggccgac ggcgacgact ggtcgtacag aagccattca tcggcgcgga
     1381 acggacgcat gaaccacatc gcgtgatcca gcgacgccac catcagatgc ttgcgctcct
     1441 cgaggtgagt cacctgtgcc gagcccaaca acgtgaggtc gctcatgtag gccagcgcgc
     1501 agatgtgcag caccggatcg tcgggcagcg ggtcgcggtg ccggaaccac acctgctgtt
     1561 gtgacgcctt gccgggcagc agcttcagct cgtcgcgcgg caccatgcgc acatcccact
     1621 ccgcgaactg ctggaatccg gcttcgtcga acacgccgcc cttggagatg aaattgggca
     1681 gatcgtcggg cggcaccgcg aacggggcgg cgtcctggtg ctcgatcccg ctctggtcgg
     1741 tctggaacga cgccgacatc ga
//
LOCUS       DASYLX010000508         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2437482,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000508 DASYLX010000000
VERSION     DASYLX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437482"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="lhgO"
                     /locus_tag="VGP27_12275"
     CDS             <1..1131
                     /gene="lhgO"
                     /locus_tag="VGP27_12275"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEV7852111.1"
                     /translation="VTLFEKEDRLASHQTGRNSGVVHAGLYYEPGSNKAVLCRRGVGL
                     LEEFCEQHGIRRIACGKVLVALDDVERARLDNIRERALANGVSGVRIIGPDELRELEP
                     HVRGVAALHSPSTSIVNFAEVTQALAADATADGAKILLGHEVVGLRTSNTEVALTARG
                     ASGSTEAVFDRIVVCGGLQSDRLAELAGDGPDPVIMPFRGEYYALKPERRNLVNGLVY
                     PVPDPRYPFLGVHVTPRVDGEVVIGPNAVLALAREGYTWGRLSIPDMLEVVGSPAFWR
                     FARQHWRTGIKEMRGSLSKKRFIAAARAYVPELGDDDVVPGGAGVRAQALDSDGGLVD
                     DFRISIRERVVLLRNAPSPAATSSLAIAEHIVTTMENGRRWE"
     gene            1128..2024
                     /locus_tag="VGP27_12280"
     CDS             1128..2024
                     /locus_tag="VGP27_12280"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7852112.1"
                     /translation="MTRPLSRGLWGVLATPFDDGLAVDLDSLRNEVASFAADGAGGLV
                     ALGVFGEAAALNPDEANSVAHTVASSTDVPFVLGLSERDPDAVVRQARRLLDAVPRSP
                     VALMAQIADADPAAAAASLRRLYEATEVGILIQDYPVVSGVSVTDEQVVEIVSSCPFA
                     VGVKSETPPTSVAVAHLASNLGVPVFGGLGGVGLLDELAAGSAGAMTGFSHPAALAAV
                     LRAYHDGGFEAARDAWAPWLPLANFEGQLRVGLSIRKELLRRRGVIASGRVRPPALTL
                     PTALIPILEKHLATLPTTTVTR"
     gene            2035..2823
                     /locus_tag="VGP27_12285"
     CDS             2035..2823
                     /locus_tag="VGP27_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852113.1"
                     /translation="MDLGITGRTALVLGASGGLGSAIAVRLAQEGANVAAAGRSAEKL
                     TDTVKRIEAAGVKALPLRWDLGDLDSVDAHVSEIEKALGPVDILVNNTGGPPPTPAAG
                     QDPATWRSSFDQMILPVIVITDRVLPGMRERGWGRILTSTSGGALSPIPNLAISNTLR
                     ASLHAWSKTLSDEVGRDGVTSNVIVPGRIATARTQFLDESRAEREDQSIDDVMAASAA
                     TMAVGRYGRPDEYADVAAFLCSERASYVTGSVIRVDGGQLKSVI"
     gene            2835..>3601
                     /locus_tag="VGP27_12290"
     CDS             2835..>3601
                     /locus_tag="VGP27_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7852114.1"
                     /translation="MRIRTLQVDGAACVTLADGRLARADRILDGAPSDLFSLLQAGAL
                     DALAAAVARGVDDASCVDPRSPVAAPWTRPRKILGIGLNYGAHAGDLGEQAPRTSPAS
                     FIKGDHTIVGPDQPIVVPPGIGRVTAEAELGLVIGRTCFQVGVDEALSYVAGVVPVLD
                     QTAEEILMENPRYLTRVKNYPSFFSFGPDLITLDEVLSHGPLSGLRVATVHNGAVHRK
                     DTVAGMTFSPAELLSFHSQVMPFYPGDILSTGTPGAVP"
BASE COUNT          562 a         1249 c         1198 g          592 t
ORIGIN      
        1 gtgacgctgt tcgagaagga ggaccgcctc gcgtcgcacc agaccgggcg caacagcggt
       61 gtcgtgcacg cgggcctgta ctacgagccc ggctcgaaca aggcggtgct ctgccgccgc
      121 ggcgtcggtc tcctcgagga gttctgcgaa cagcacggta tccggcggat cgcctgcggc
      181 aaggtcctcg tcgcgctcga cgacgtcgaa cgcgcgcggc tcgacaacat cagggagcgg
      241 gcattggcca acggtgtctc cggtgtgcgg atcatcggcc ccgacgagct acgcgaactg
      301 gagccgcatg tgcgtggcgt cgccgccctg cactcgccgt ccacctcgat cgtgaacttc
      361 gcggaggtga cgcaggcgct cgccgccgac gcgaccgccg acggcgcgaa gatcttgctc
      421 gggcacgaag tcgtcgggct ccggacatcg aacacggagg tcgcgctaac cgcccgcggc
      481 gcaagcggtt ccactgaggc cgtgttcgac cggatcgtgg tctgcggcgg cctgcagagc
      541 gaccggctcg cggagctggc gggcgacggc ccagacccgg tgatcatgcc ttttcgcggc
      601 gagtactacg cgctcaagcc ggaacgccgc aacctcgtca acgggctcgt ctatccggtg
      661 cccgatcctc gctacccgtt cctgggtgta catgtcaccc cgcgcgtcga cggcgaggta
      721 gtcatcggcc ccaacgccgt actcgccctg gcacgcgagg ggtacacgtg gggcaggctg
      781 tcaatccccg atatgctcga agtcgtaggc tcaccggcat tttggcggtt cgcccgacag
      841 cattggcgaa ccggtatcaa ggagatgcga ggatcgctga gcaagaagcg attcatcgcc
      901 gccgcccgcg catatgtgcc cgaactcggc gacgacgacg tcgttcccgg gggcgccggc
      961 gtccgcgcgc aggcactcga ttccgatggc ggcctcgtcg acgacttccg gatcagcatc
     1021 cgcgagcgtg tcgtcctcct gcgcaacgcc ccgtcaccag cggccacgtc ttcgctcgcg
     1081 atcgccgagc acatcgtgac aacgatggaa aacggaaggc gatgggagtg acccggccgc
     1141 tgtcccgagg tctttggggg gtcctcgcca cgcccttcga tgacggcctc gctgtcgact
     1201 tggattcgtt gcgcaacgag gtcgcctcgt tcgccgccga cggtgcaggc gggctggtcg
     1261 cactgggcgt cttcggcgag gcggcggcgc tgaaccccga cgaggccaac agcgttgcgc
     1321 acaccgtcgc atcctcgacc gacgtgccgt tcgtgctcgg cctgtccgaa cgtgaccccg
     1381 acgccgtcgt ccgccaggcg cgccggctgc tcgacgcggt accgcgctcg ccggttgcgt
     1441 tgatggcaca gatcgcagac gccgatccgg ccgccgccgc cgcttcgctt cgccgtctct
     1501 acgaggcgac ggaggtcggc atcctcattc aggactaccc ggtcgtgtcg ggagtcagcg
     1561 tcaccgacga gcaggtcgtc gagatcgtgt cttcgtgccc cttcgctgtc ggcgtgaagt
     1621 cggagacacc gccgacctcc gttgcggtcg cccacctcgc atccaacctc ggcgttccgg
     1681 tcttcggcgg ccttggcggc gtcggactgc tcgacgagct ggccgcgggt tcggccggtg
     1741 cgatgaccgg attcagccat cccgcggcat tggcggccgt gttgcgcgcc tatcacgacg
     1801 gcggcttcga agccgcccgc gacgcgtggg cgccatggct tccgctcgcc aacttcgaag
     1861 gacagctgcg ggtgggcctt tcgatccgta aagaactgct gcggcgacgc ggcgtgatcg
     1921 cctccggccg ggtgcgtcca cccgcactga cactgccgac cgcgctgatt cccatcctcg
     1981 agaagcatct cgcgaccctg cccaccacca ccgtgacccg ctgaaggagc caccatggat
     2041 ctcggcatca ccggacgcac cgcgctcgtg ctcggcgcca gcggcggcct cggcagcgcc
     2101 atcgcggtgc ggctcgccca ggagggcgcc aatgtcgcgg ctgcgggccg atccgccgaa
     2161 aagctcaccg acactgtgaa gcggatcgag gccgcggggg taaaggccct gccgctgcgc
     2221 tgggatctgg gggacctcga ttctgtggac gcgcacgtct cagaaatcga aaaagccctc
     2281 ggcccggtcg acattctggt caacaacacc ggcgggccgc cgcccacacc cgccgcgggc
     2341 caggaccccg ccacctggcg cagcagcttc gaccagatga tcctgccggt catcgtcatc
     2401 acggatcggg tgttgcctgg aatgcgcgag cgtggctggg gccgcatcct gacgtcgacg
     2461 tccggcgggg cgctcagtcc cattcccaat ctcgcgatat ccaacacgtt gcgggccagc
     2521 ctgcacgcct ggtccaagac cctctccgac gaggtgggtc gtgacggcgt gacgtcgaac
     2581 gtcattgtgc caggccgaat cgccactgcc cgaacgcaat tcctcgacga aagcagggcg
     2641 gaacgagaag accagagcat cgacgacgtc atggccgcca gcgcggcgac gatggcggtc
     2701 ggccgctatg gccgcccgga cgaatacgcg gacgtggccg ctttcctgtg cagcgagcgg
     2761 gcgtcctatg tgaccggctc ggtcatccgt gtcgacggcg gtcagctcaa gagcgtcatc
     2821 tagaagggat tgttttgcgc atccgcacac tgcaggtcga tggcgcggca tgcgtcacgc
     2881 tggccgacgg ccgactcgcc cgcgccgacc ggatactcga cggtgcaccg tccgatctgt
     2941 tctcactcct gcaggccggg gcgctcgacg ccctcgccgc ggcggtggcg cgtggtgtgg
     3001 acgacgcctc atgcgtggat ccgcggtctc cggttgccgc gccatggacc aggccacgca
     3061 agattctcgg catcgggctc aactacggcg cgcatgcggg cgatctcggc gagcaggctc
     3121 cacgcacgtc gcctgcgtcg ttcatcaagg gtgaccacac catcgtcgga ccggaccaac
     3181 cgatcgtcgt cccgccgggt atcggtcgag tcacggcgga agccgaactg ggtctcgtca
     3241 tcgggcgcac gtgttttcag gtcggagtcg acgaagcgct gtcctacgtt gcgggtgtcg
     3301 tgccggtgct cgaccagacc gcggaggaga tcctgatgga gaatccgcga tacctgaccc
     3361 gggtgaagaa ctatccctcg ttcttctcgt tcgggccgga tctgatcacg ctcgacgagg
     3421 tgctctcgca cggaccgctg tcggggctga gggtcgcgac cgtgcacaac ggtgcggtgc
     3481 accgcaaaga cactgtcgcc ggcatgacgt tctcgcccgc cgaattactg agcttccaca
     3541 gccaggtgat gccgttctat ccgggcgaca tcctgtcgac gggaacgccg ggcgccgtgc
     3601 c
//
LOCUS       DASYLX010000509        11307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1779640,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000509 DASYLX010000000
VERSION     DASYLX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779640"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGP27_12295"
     CDS             complement(<1..284)
                     /locus_tag="VGP27_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852115.1"
                     /translation="MSLVGECALVGQAIQRAAALVSPHAPTSGGVGLGDIEGAARSAN
                     AAARMTNGSSGVLIAVHRRFAVDNAAALSASGYADSALQARMATAAALTHA"
     gene            373..696
                     /locus_tag="VGP27_12300"
     CDS             373..696
                     /locus_tag="VGP27_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEV7852116.1"
                     /translation="MTVYALNLFDIANRDEYLAYSKRSPQEVGKHGGRVIALGQFRES
                     ITGDIEPRKVLIVVEWDSKDAFQSYCDDPELADLHAHREAGSSSYIWHLFDHLDDLRP
                     LLKRQ"
     gene            805..1248
                     /locus_tag="VGP27_12305"
     CDS             805..1248
                     /locus_tag="VGP27_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852117.1"
                     /translation="MSRVSRFTASVVVSGAVAMTGLTLGAGTSTAEGPSYQGGNCPQG
                     MTCTHWCPGDPAIPGSQVLSWDWNVCHDWYWNSHGIVDVDSSTIYPWSGVPHQVAPPA
                     PVAPAPPPEPLPPGTPFCSPRGALIIIPPICDEIGVDLPPESVRN"
     gene            complement(1273..1857)
                     /locus_tag="VGP27_12310"
     CDS             complement(1273..1857)
                     /locus_tag="VGP27_12310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV7852118.1"
                     /translation="MVTVSACGSGQDTPSASTSSSLTSSTVDVSTASTTETATAAGTC
                     PTAAPQGGGAPEWTLPGATGSVAVTGSTDTTAPSVKVDGPFSVTETQVHTLRAGGGAV
                     VADTATVSVCYMGVNGRDGTVFDSSYQQGAPVDFPLDRVVPGFQKAIAGQKVGSTVAV
                     AMTSADGYPEGQPSAGIQQGDTLVFAIKILSAST"
     gene            1790..1939
                     /locus_tag="VGP27_12315"
     CDS             1790..1939
                     /locus_tag="VGP27_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852119.1"
                     /translation="MSEDDVLADGVSWPEPHAETVTMNDAAQAANAAKEEIREEFTGA
                     HATAE"
     gene            2116..2676
                     /locus_tag="VGP27_12320"
     CDS             2116..2676
                     /locus_tag="VGP27_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7852120.1"
                     /translation="MLRVIYSMSVSLDGFIAGPDGDIGWGDPDAGLMRFHIEQTRDIA
                     AYLCGRRLYEDMLVWETAEQTMSDDADLEFARIWRPIPKLVFSRTLNSVEGNARLAAD
                     DVVTEVARLRDQPGEGVVSIGGASLAAAAIAEDLIDEYRQFVNPVVLGGGTPYFPPLA
                     KRLDLRLTESRTFGTGAVYLRYQRTR"
     gene            2766..3227
                     /locus_tag="VGP27_12325"
     CDS             2766..3227
                     /locus_tag="VGP27_12325"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEV7852121.1"
                     /translation="MATKKAAGAKSASKNVAVRAPVKKAPTAKAAVVGAARRGRVAAD
                     DPLSEQRVLYLTNVFSRAQLAKLVGVSPSQTSRWASGEERPGPAAAPALIDLEHVYSR
                     ARLVWGGDSARIWLESANAFLGGARPLDVLVTEGAARVLQTLDAEMWGGAA"
     gene            3228..3782
                     /locus_tag="VGP27_12330"
     CDS             3228..3782
                     /locus_tag="VGP27_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEV7852122.1"
                     /translation="MLIYRVFPYLETAPLGEPGHPLYEHRPQRGGRADHPDYYVWYVA
                     RQSEAACGEAFGNLSRWIDSMLDFPAVPGARKTLGVYELPDDLRICHLDDPHRLVELG
                     LRPTQIVTRNLAVTSEWAHRIWSQHSPAVDDRKWQAVQWWSFHRPTWTVVASWERPKL
                     VELEHLNLDHPAVVAAAKSLNRPL"
     gene            complement(4428..4910)
                     /locus_tag="VGP27_12335"
     CDS             complement(4428..4910)
                     /locus_tag="VGP27_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7852123.1"
                     /translation="MEPNWLNAREDRAWRAFMHAHHQLVVHLNRRLQESGLSGADYEV
                     LAVLSALDRDRMPARDLGNALGWEKSRVSHQLRRMEKDGLISREPNPGDARSTLVCLR
                     PTGRAAIEKAAPRHVEDVRRNFIDLLTPAELDMVTTLNERVLHHLATNDDSPAADEFS
                     "
     gene            5003..5626
                     /locus_tag="VGP27_12340"
     CDS             5003..5626
                     /locus_tag="VGP27_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7852124.1"
                     /translation="MSSISIIGSGNMASAIGALAVKGGNAVEVVARDATKAAALAEAL
                     GNGATAGTWGAAPAGEIVILAVMFDSAVPVVSQYGDALAGKIIVDITNPFNASGTGLA
                     IPHDTSIAQMVAEAAPAGAHVVKAFNTLFRDVLAAGGPLDVFIAGDDAQAKARLSAFI
                     ESLGLRPMDAGQLPMARALENAGLLEMGLVANSVKHTNFFLGVNILS"
     gene            5659..6603
                     /locus_tag="VGP27_12345"
     CDS             5659..6603
                     /locus_tag="VGP27_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852125.1"
                     /translation="MRVFVTGGTGHSGSHIIPELIAAGHEVTGLARSDTAAAAVSALG
                     ARVRRGSLEDLDGLKEAAADSDGVIHVAHRQDLLPSGGIDAVTAAELPVILAYGEALA
                     GTGKPLVAAGSMGSPGNLGRPATEEDPALPSGDEYKGTLRFRNAVETAVVGLAKRGVR
                     SSIVRLPTIAHSTTDRAGFLPGLIALAKEKGFAGYPGDGANLWGAVHVRDVASLFRLA
                     LEKGPAGKYWHAIEDGGIPFREIAEAIGSRLGLPTVSVPADVLMLPGYFGMFTNLVTM
                     SLPASNLITRRTLGWEPAQPRLLADLDNGNYFSGQLTS"
     gene            7054..7731
                     /locus_tag="VGP27_12350"
     CDS             7054..7731
                     /locus_tag="VGP27_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7852126.1"
                     /translation="MRLLVVEDEQRLASGLRKGLEAEGFAVDVVHNGTDGIWMARENP
                     FDAIILDVMLPGANGYQVCRTLRGEGNWTPILMLTAKDGVWDEVEGLDTGADDYLPKP
                     FSYAVLVARLRALRRRGARPRPTVLEAGGLRLDPATRRVWHRGEQVELTRREFAILEY
                     FLRHPDEVLAKRDILDCVWDFDFDGDPNIVEVYVRRLRIKLLRPGDGPLIQTVRGAGY
                     RLIAAGD"
     gene            7766..9061
                     /locus_tag="VGP27_12355"
     CDS             7766..9061
                     /locus_tag="VGP27_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7852127.1"
                     /translation="MATIVVALALTVGAIALIAAARRTMLDEVTEAAQKQAAEVVSQL
                     ESGRLPVLEVAGSDEQLIQVITPAGEVVASSRNVAGLPVVARLAPGQSAEAVTPLDND
                     AFIAVAEGAHTANGQRTVLVARALVDVFDTTKAITRWLIIGLPLMVAVVALTTWFAVG
                     RALAPVEAIRREVDEISAAQLHRRVPQPKAQDEVGRLAATMNRMLARLESARNSQRRF
                     VSDASHELRSPITTIRQHAEVALAHPDRMTAQELADVVLAEQYRMQGLVEDLLLLARA
                     DEHVPLSRAPVDLDDLAFEEGHRLRSATSKRVDTSRVGAARVRGDADALRRMVRNLGE
                     NAARHASSRVDIALADRDGAVVLTVDDDGPGIPETERERVLQRFVRLDEARSRDDGGS
                     GLGLSIVDEVVRAHGGSISITQSALGGARIQVTVPADGR"
     gene            9108..9443
                     /locus_tag="VGP27_12360"
     CDS             9108..9443
                     /locus_tag="VGP27_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852128.1"
                     /translation="MNIIKSIGIVTAVGAAAVTIGAGIAMAQPDDGEGTPITGDALTK
                     ASQTALAYTGGGTVTGSELGDEESYYQVEVTLADGRQTDVQLDPNFVVVNSKTEPASA
                     DNEPDAGGN"
     gene            9656..9769
                     /locus_tag="VGP27_12365"
                     /pseudo
     CDS             9656..9769
                     /locus_tag="VGP27_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(9789..10196)
                     /locus_tag="VGP27_12370"
     CDS             complement(9789..10196)
                     /locus_tag="VGP27_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV7852129.1"
                     /translation="MIYLDTSALVKLIRIEAESDALGDWLDQHTELRWITSTLAEVEL
                     PRAIRAIAPEGLSAVPSVLARLDRFEIDSVVRATAAAYQDPVLRSLDAIHLATAQTAA
                     STAPLTALVTYDGRLLDAAHGLGMTVVAPGRTS"
     gene            complement(10200..10472)
                     /locus_tag="VGP27_12375"
     CDS             complement(10200..10472)
                     /locus_tag="VGP27_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007410641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV7852130.1"
                     /translation="MSEVSIRMLNQETSKVLARVKRGEEITLTDRGEVIARIVPAQAG
                     PLDALISTGRVQPATVRGPAPRPTIPMRGDLDAGTLLQRMRADERY"
     gene            complement(10671..10829)
                     /locus_tag="VGP27_12380"
     CDS             complement(10671..10829)
                     /locus_tag="VGP27_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852131.1"
                     /translation="MVGTYDTGEPVANLGREPEHDVPGPVVDGGGGDLAVGVSFEDGH
                     AFDLGASR"
     gene            10819..11142
                     /locus_tag="VGP27_12385"
     CDS             10819..11142
                     /locus_tag="VGP27_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852132.1"
                     /translation="MPTISSRRQHDRDACNLFDVPDGGHTLRRKFDTLAAHCATVGRH
                     YADIERTVTTALEPDESAEELVDRCRTLNGLGADHVVLIGRGRPWQPTDLDTIARTVT
                     QLAAA"
BASE COUNT         2005 a         3615 c         3735 g         1952 t
ORIGIN      
        1 gcgtgggtga gtgcggcggc cgttgccata cgcgcttgca gcgcgctgtc ggcgtacccg
       61 gacgcactca acgcggccgc attatcgacc gcgaacctcc tgtgcacggc gatcagcact
      121 cccgacgaac cgtttgtcat ccgcgctgcg gcattcgcgg atcgcgccgc accctcgatg
      181 tcgccaagcc cgacgcctcc ggaagtaggc gcgtgcgggg acaccaaagc cgcagcccgc
      241 tggatcgcct gccctacgag tgcacattca cccacgagag acatcactca attatcgagc
      301 catcggtgag ccgctccacc gccaattttc atgcggcgga agggcgggca cccgcggtgt
      361 actgtcacgc cggtgacggt gtatgcgctg aacctgttcg atatcgccaa ccgcgacgag
      421 tatcttgcgt actcgaagcg ctcaccccaa gaggtcggca agcacggcgg tcgcgtcatc
      481 gccctcgggc agttccgcga atcgatcacc ggcgacatcg aaccgcgcaa ggtgctgatt
      541 gtcgtcgagt gggattcgaa ggatgccttc caaagctact gcgacgatcc cgaactggcc
      601 gatctgcatg cccaccggga ggctggttcg tcgtcctaca tctggcacct gttcgaccac
      661 ctcgacgacc tgcgaccgct gctcaagcgt cagtgattgc agccaagcct cctgccgttg
      721 ttgggatcgg tagcgggcgg cttcggattt actcggctgc accggcgatt gccaatgggg
      781 cggcgcccca acgtaaggac tcgtatgagc cgagtctcgc ggttcacggc gtcagtggtg
      841 gtgtccggcg ccgtcgcaat gaccggtctg acactgggtg cgggcacctc gacagctgag
      901 gggcccagct atcaaggtgg gaactgcccg caggggatga cctgtaccca ctggtgtccc
      961 ggcgaccccg ctataccggg cagccaagtg ctgagctggg attggaacgt ctgccacgac
     1021 tggtactgga actcccacgg catcgtcgac gtcgacagca gcacgatcta tccgtggtct
     1081 ggcgtacctc atcaggtggc cccgccagcg ccggtcgcgc ctgcgccgcc gccggagccg
     1141 ctgccgccgg gcacaccgtt ctgcagcccg cgcggagcgt tgatcatcat tccgccgatc
     1201 tgcgacgaga tcggtgtcga cctgccgcct gaatcggtac gcaattagac agagacgccc
     1261 agaccttgtg actcaggtcg aggcgctgag gatcttgatc gcgaagacga gcgtgtctcc
     1321 ctgctggata cccgcgctgg gctggccttc ggggtagccg tcggcagagg tcatggcgac
     1381 ggcgaccgtt gaccccactt tttgtccggc gatcgccttc tggaagccgg gcacgacgcg
     1441 gtcgagtggg aaatcgactg gggcgccctg ttggtaactg ctgtcgaaca cggtcccgtc
     1501 acgtccattg acgcccatgt agcagaccga aaccgttgcc gtgtccgcca cgaccgcccc
     1561 tccaccggcc cgcaacgtgt gcacctgtgt ctcggtcacg ctgaacggac cgtccacctt
     1621 gacgctgggc gccgtcgtat ccgtggatcc ggtgaccgcg acgctgccgg tggccccggg
     1681 cagcgtccac tcgggtgcgc caccgccctg cggtgccgcc gtcgggcacg tgccggcagc
     1741 ggtggccgtt tcggtggtgg acgccgtgct cacgtcgaca gtggaggacg tgagcgagga
     1801 cgacgtgctt gccgacggcg tgtcctggcc ggagccgcac gcggagaccg tcacgatgaa
     1861 cgacgcggca caggcggcaa acgcggcgaa ggaagagatt cgagaggagt tcacgggcgc
     1921 tcacgctaca gccgaatagc cgaacgatga attcgtcctg ccgtcgaggt ctctctgttc
     1981 ggaacaacga aacggcttga agggtagaag tgactgcacc gaaggcttcc tcggcggtta
     2041 cctgccgatc aacgtgtcgg tatctcgggg gagtgcggca agacgactac cgctaccgtc
     2101 gcaaaggaga acctgatgct gcgcgtgatc tactcgatga gtgtgtcgct ggacggtttc
     2161 atcgccggcc cggatggtga catcggctgg ggggatcctg acgccgggct gatgcgcttc
     2221 cacatcgagc agacccgcga catcgccgcg tacctatgcg ggcgccgcct gtacgaggac
     2281 atgctggtat gggagaccgc cgaacagacc atgtccgatg acgcggacct cgaattcgcc
     2341 cggatctggc ggccgatccc aaagttggtg ttctcccgaa cgctgaattc ggtggaaggc
     2401 aacgcgcggc tggccgccga cgacgtcgtc accgaggtcg cccggctgcg ggaccagccc
     2461 ggtgagggcg tggtgtccat cggcggggcg agcctcgccg cggccgccat cgccgaggac
     2521 ctgatcgatg agtaccgcca gttcgtcaac ccggtcgttc tcggtggcgg caccccctac
     2581 ttcccgccgc ttgcaaaacg tctcgacttg cggctgaccg agtcccgaac gttcggcacg
     2641 ggcgcggtct accttcgcta tcaacgcaca cgctgagccg gcgaccggtc aagttgtggc
     2701 aggggagagt cagtgcccag ttgctcgtcg cgctgcccac gaaaaaaatt gggtagagtg
     2761 atgatatggc tacaaagaag gctgccggcg caaagagtgc atcaaagaac gtggccgtca
     2821 gagctccggt gaagaaggcg cccaccgcga aggcggccgt agtcggggct gcgcggaggg
     2881 gccgggtcgc cgctgacgat cccttgtctg aacagcgggt cctgtacctc acaaacgtgt
     2941 tctcgcgtgc acagctggct aagttggttg gcgtcagccc atcacagact tcccgatggg
     3001 catcgggtga ggagcgtcct ggccccgcgg cagctccggc gctaattgat ctagaacatg
     3061 tgtattcgcg ggcgcgcttg gtatggggag gcgactcagc gcgtatctgg ctggaaagcg
     3121 ctaacgcctt cctcggggga gcccgaccgc ttgatgttct agttaccgaa ggagccgctc
     3181 gggttcttca aacgctcgac gcagagatgt ggggcggggc ggcgtgagtg ctgatatatc
     3241 gcgtctttcc ctaccttgaa acggcgccgc tcggcgagcc agggcatccg ctgtatgaac
     3301 accgtccgca gcgcggtggt cgggctgatc accccgacta ttacgtatgg tatgtagccc
     3361 gccaatccga agccgcgtgc ggcgaagcgt tcggcaacct gtcgcgttgg atcgactcga
     3421 tgttggattt tccggcagta ccgggtgcac ggaagacgct cggggtgtac gaactgcccg
     3481 acgatctgcg catttgccac cttgatgatc cgcaccgcct agtagagcta gggcttcgac
     3541 cgacacagat cgtcactcgc aacctggcag tcacttcgga gtgggcgcac cggatctggt
     3601 ctcagcattc tcccgccgtt gatgaccgaa aatggcaggc ggtgcagtgg tggtcgttcc
     3661 atcggccaac ttggactgtc gtcgccagtt gggaacggcc caagctcgtc gaactcgagc
     3721 atctgaacct cgaccaccca gccgttgtcg cggcagctaa gagcctcaac cggcctctat
     3781 agagaatctg gctccggaag cggatcggcg acctaccgga cgccctccag gcttacgcgc
     3841 ataatcgtca tcggaatgaa aactgctgga ccgcagagca atacggcata cattactggt
     3901 aatccgccgt attcgtttcc tccgtattcc acctgggcct tcggcccatt gccaggcgag
     3961 gggagagccc gatcctgccg aggacgctta ctcaatcggc aatcaggata gttttcaggg
     4021 cgttgccgcg gcagtggtca tgcagggcag tgggggcgtc gtcgaaactg gccacattgg
     4081 ttgttactac ttcgggctgg aattggccgc tggcgattag agcgaagggc gtcggggatc
     4141 agagttcgga tgtgagcgcg gccgatgtgc agggtgacgt tacggatgta ttctgcggcc
     4201 aacgggatga cgccgcgccg gtgcagcgac catacgcacg agcagatgcc ttcgggtgca
     4261 gtgtgtttca accgacggtg atcttctgcc agtcagagat gccgtcaggg aaattggtga
     4321 ggagtttctc tactggtatt aaaagggggc gtctgcctgg ctcttcaggg cgatccctat
     4381 caaggacctg ggatgcgggg tgctacatcg ctcacggcta gtgaaggtta tgaaaactcg
     4441 tctgcggccg gggagtcgtc gtttgtggcg aggtggtgca ggactcgttc gttgagggtg
     4501 gtgaccatgt cgagctcagc cggggtaagc aggtcgatga agttccggcg gacgtcctcc
     4561 acgtgcctgg gtgccgcctt ctcgatggcg gcgcggccgg tcggccgaag gcagaccaag
     4621 gtgctgcggg cgtcgccggg gttgggctcg cggctgatca gcccgtcctt ctccatgcgc
     4681 cgcagctggt gggagacacg gctcttctcc cagcccagtg cgttgcccag gtcccgggcg
     4741 ggcatgcggt cccggtcgag agccgagagc accgcaagga cctcgtagtc ggccccggac
     4801 aggcccgatt cctgcagccg ccggttcagg tgcacgacga gctgatgatg cgcgtgcatg
     4861 aaggctcgcc aggcacggtc ttcacgagcg ttcagccagt tcggttccat cggctacatg
     4921 ctaaaccctg attggttgac gcatcaccaa tctctgctta tggttgagga gtcaaccttt
     4981 tgaagagcag atgggataaa tcatgagcag catcagcatc atcggatcgg ggaatatggc
     5041 cagcgccatc ggcgcccttg ccgtcaaggg cggcaacgcc gtcgaagtcg tggctcgcga
     5101 tgcgaccaag gccgcggccc tggccgaggc gctcggcaac ggcgccacgg ccggaacatg
     5161 gggcgccgcc ccggccggcg agatcgtcat cctcgccgtg atgttcgaca gcgccgtgcc
     5221 ggtcgtcagc cagtacgggg acgcgctggc tggcaagatc atcgtcgaca tcaccaaccc
     5281 cttcaacgcc agcggcaccg ggctggccat ccctcacgac acctccatcg cgcaaatggt
     5341 cgccgaggcc gcccccgcag gcgcccatgt tgtgaaggcg ttcaacaccc tcttccgcga
     5401 cgtcctggcc gcgggcggcc cattggacgt gttcatcgcc ggcgacgacg cgcaggcgaa
     5461 ggcacgcctg tcggcattca tcgagagcct cggactgcgc ccgatggacg ccgggcagct
     5521 gccaatggcg cgagcactgg agaacgccgg cctgctggag atgggcctcg tcgccaactc
     5581 cgtcaagcac accaacttct tcctcggcgt caacattctc agttgatcag ctccacctac
     5641 cctcagaagg actgcagcat gcgcgttttc gtcactggcg gcaccggcca ctccggttcg
     5701 cacatcatcc ccgagctcat tgccgccggc cacgaggtca ccggcctggc ccggtcggac
     5761 acagccgcgg cggcggtgtc cgcgctcggc gcgagggtgc gtcgcggcag cctcgaggat
     5821 ctcgacgggc tcaaggaggc ggccgctgac tccgacggcg tcatccacgt cgcacaccgg
     5881 caagacctgc ttccgtccgg tgggattgac gccgtgaccg ccgcggagct cccagtcata
     5941 ctcgcgtacg gcgaggcact ggcgggaacc ggaaagccgc tcgtcgcggc cggcagtatg
     6001 ggctcgcccg ggaacctggg ccggccggcc accgaagagg acccggccct tcccagcggg
     6061 gatgagtaca agggcaccct acggttccgt aacgccgtgg agaccgccgt agtcggcctc
     6121 gccaagcggg gagtgcggtc ttcgatcgtg cggcttccca cgatcgcgca cagcacgacc
     6181 gatcgtgccg gcttcctccc gggtctcatt gcgctcgcga aggagaaggg cttcgccggc
     6241 taccccggag acggcgcaaa cctgtggggc gctgtgcacg tccgcgatgt cgcctccctg
     6301 tttcgcttgg cgttggagaa gggtccggcc ggcaaatatt ggcatgcgat cgaggacggg
     6361 ggcatcccgt tccgcgagat cgccgaggcc attggcagcc gtctgggcct gcccaccgtg
     6421 agcgttcccg cggacgtact gatgctgccg ggatacttcg gaatgttcac gaatctggtc
     6481 acgatgtccc tgccggcctc caacctcatc acgcgccgga ccctcggctg ggagcccgct
     6541 cagcctaggc tgctcgccga tttggacaac ggcaattact tctccggaca gctgacgtcc
     6601 tgaccacgcc gcacacttcc ggctgcaaat cggagacgcc acggtcaacg gcatattctt
     6661 cgtcctgctc gacgcggacg gcaagatcgc cgaggtgagc atcttctacc gcacgctgcc
     6721 gtccggtgtc gcgctgcagc ggaaccttgc gggcgtaatc ggcatgcagc cttgggaact
     6781 gcgaacaaat gggggagtga gacgaaggac gtaagctacg ccccagggga ggcattcgga
     6841 acaccctgat tcatcagatg aaaacagagt ggttgaccgg taaagccgta tattcgaccc
     6901 cgtcgtattc gacgagcgga accgcggggg tgtggaatgc gggtccaccg tcagactccc
     6961 agtggcgaca agtgctgtcg ctgacatcat cggtgtgctg accaagcgaa ttcagccgtc
     7021 agtcagtttc aggctgggat catggcagcc acgatgcgtc tactggtggt agaagacgag
     7081 cagcggctcg cctcaggcct gcggaaaggg ctcgaggccg aaggtttcgc ggtcgatgtc
     7141 gtgcacaacg gcaccgatgg catctggatg gcgcgcgaaa acccgttcga tgcgatcatt
     7201 ctcgacgtta tgctgcccgg ggcgaacggg taccaggtgt gccgcacgtt gcgtggcgaa
     7261 gggaattgga cgccgatcct catgctgact gccaaggacg gcgtgtggga cgaggtcgag
     7321 gggctggaca ccggcgcgga cgactatctg cccaaaccgt tttcgtatgc ggtgctggtc
     7381 gcgcggctgc gggcgctgcg ccgccgtggc gcccggccgc ggccaactgt gctggaggcc
     7441 ggtggtcttc gcttggatcc ggcgacgcgg cgagtctggc atcgcggcga gcaggtcgaa
     7501 ctcactcggc gagagttcgc catcctggag tacttcctgc gccaccccga cgaggtactg
     7561 gcgaagcgcg acattctcga ctgcgtctgg gatttcgact tcgacggtga ccccaacatc
     7621 gtcgaggtgt acgtgcgcag actgcgtatc aaattgctcc ggccgggcga cggtccgctg
     7681 atccaaaccg ttcggggcgc cggctaccgg ctgatcgccg ccggtgatta ggcgcttagc
     7741 ctcgatccgg gctgtcacca cggccgtggc cacgatcgtc gtcgccctcg cactcacggt
     7801 cggcgcgata gcgctgatcg ccgccgcgcg gcgcacgatg ctggacgagg tgacggaggc
     7861 ggcccaaaag caggccgccg aggttgtcag tcaactggag tccggtcggc tacccgtcct
     7921 cgaggtggcc ggcagtgacg agcagttgat tcaggtaatc accccggctg gggaggtggt
     7981 ggcatcgagc cggaacgtcg ccggcctgcc tgtcgtagcg cggttggcac ccggacagtc
     8041 cgcagaggcc gtcacccccc tcgacaacga cgccttcatc gccgtggccg agggcgccca
     8101 cacggcaaac ggtcagcgga ccgtactggt cgcgcgggcg cttgtcgatg tcttcgacac
     8161 gaccaaagca atcacccggt ggttgatcat cgggctgccg ctgatggtgg cggttgtcgc
     8221 actcactacc tggttcgcgg tgggccgcgc gctggcacct gtcgaggcga tccggcgcga
     8281 ggtggacgag atctcggcag cgcagttgca tcgacgggtg ccgcaaccca aagcacagga
     8341 cgaggtgggc cggctggccg ccactatgaa ccggatgctg gcaaggctcg agagcgcccg
     8401 aaacagtcag cgccgcttcg tatccgacgc gtcgcacgag ttgcgctcgc cgatcaccac
     8461 catccgccag cacgccgagg tcgcgctcgc tcaccccgac cggatgacgg cccaggagct
     8521 cgccgacgtg gtgttggcag agcaataccg gatgcagggc ctcgtcgagg atctgttgct
     8581 gctggcgcgg gccgacgagc atgtgccgct gtcgcgcgca cccgttgacc tggacgacct
     8641 ggcgttcgag gaagggcatc ggctccggtc ggcgacctcg aagcgggtgg acacgtcgcg
     8701 ggtgggcgcg gcgcgggtgc ggggcgatgc cgacgcgttg cgcaggatgg tccgcaacct
     8761 cggcgagaac gcggcccggc acgcgtcgag ccgggtcgac atcgcgctcg ccgatcgtga
     8821 cggcgccgtg gtgctgaccg tcgacgacga cgggccgggc atccccgaaa ccgaacgcga
     8881 gcgggtcctg cagcggttcg tccgactcga cgaagcccgc tcgcgcgatg acggcggcag
     8941 cggcctgggc ctgtcgatcg tcgacgaggt cgtcagggct cacggtggct cgatatcgat
     9001 tacccagtcc gcgctcggcg gcgcccgcat ccaggtcacg gtgccggctg acgggcgttg
     9061 aggagcgttc aggttgctgt caggtagttc gcggcaacct gtccatcatg aacatcatca
     9121 agagcattgg aatcgtgacg gccgtcggag cggcggcggt caccatcggg gccggaatcg
     9181 ccatggccca gccggacgac ggggagggta cgcccatcac cggtgacgcc ctgacgaaag
     9241 ccagccagac cgcgttggcc tataccggcg gtggcacggt gactggcagc gagcttgggg
     9301 acgaggagag ctattaccag gtggaggtca ccctggccga cggccggcag accgacgtcc
     9361 aactggaccc gaatttcgtc gtggttaaca gcaagaccga gcccgcgagc gccgataacg
     9421 aacccgacgc cggcggcaac tagcaaatgc atgccaccgg tcgcgggcta gccacgctag
     9481 cgatctgcac gccgatcgtc tccgcactca gcgtcgcgtg cagcggcggc ccggcaccgg
     9541 tggccgcgga ctgcatcaag gtcgtctctg aatccggcga gcgcaccgac gcgtgcctgc
     9601 cggtcgcgcc ggacagcgag cgtatcgtct ggacgtgtgg cgtcgccacc tcgcatcgaa
     9661 ggaagcgctc ttagacgcag gcatcgatcg ccaactcgag ggcccgcagg cgatgcacga
     9721 catcggcgca ctgttcgccg ggctggacga cctgcgctcg gaatcaacgg caccccggtc
     9781 gcatgctttc acgatgtcct gcccggtgcg acgacggtca tccccaggcc gtgggctgcg
     9841 tcaaggagtc gaccgtcata tgtcactagg gccgtcaaag gtgcagtcga tgcggcggtt
     9901 tgagcagtgg ccaggtgtat cgcgtccagt gagcgaagca ctgggtcttg gtaggccgcc
     9961 gcagtggcgc ggacgaccga gtcgatctcg aaccggtcca acctggccaa cacggacggc
    10021 acagcagaca gcccttccgg ggcgattgcc cggatggccc tcgggagctc aacttcggcc
    10081 aaagtcgatg ttatccaacg taattcggtg tgctgatcta gccagtcgcc cagagcgtcc
    10141 gactcggctt cgatgcgaat cagcttgacc agcgccgagg tatccaggta gatcacgggc
    10201 tagtaccgtt cgtcggcgcg catccgctgt agcagcgtgc cggcatcgag atctccgcgc
    10261 attggaatcg ttgggcgggg cgccgggccg cggaccgtcg ccggttgcac cctgccggta
    10321 ctgatcagcg cgtcgagagg gccggcttgc gccgggacta tgcgcgcgat cacctcgccg
    10381 cgatcagtaa gggtgatctc ctcgccgcgc ttcacacggg ccaacacctt tgacgtctct
    10441 tggttgagca ttcgtatgga cacttcactc acaccagtat cgtactacga atttgttcta
    10501 cacactattg gtgtggtcgg cttgagccgg atgtgcgaat actgaatgaa ccgcttccat
    10561 ggcgacgaga aacgagccaa cgctgatcat catgggcacg aagccgaccg cacagatgat
    10621 cgcgcgaacc caagccaccg gaaaggtttg gggtagcaac acaatatgag tcagcgggag
    10681 gcaccgagat caaaggcgtg accatcctcg aacgaaacgc cgacggcaag atcgccgccg
    10741 ccgccatcca ccaccggtcc cgggacgtcg tgctccggtt cgcggccgag attcgcgacc
    10801 ggctcgccgg tgtcataggt gccgaccatt tcctcgaggc gacagcatga ccgcgacgcc
    10861 tgcaacctgt tcgacgtccc ggacggggga catactcttc gacgcaagtt cgacactctt
    10921 gccgcgcact gcgccaccgt gggtcggcac tacgcggata ttgaacgcac cgttaccacc
    10981 gcgttagaac ccgacgagtc tgccgaagag ctggtcgacc ggtgccgcac cctcaacggt
    11041 cttggcgccg accatgtcgt tctcatcggc cgcggacgac cgtggcagcc caccgacctc
    11101 gacaccatcg cccgcaccgt cacgcagctc gccgcggcct aattgtctgg cgggcaaggc
    11161 accagaacaa ggcggctgat cccggatcgc gcggccacac cctgcgcggt ggactccaga
    11221 ggtcggtaga acaccagcag caggacacca accgcgatgg caagactgag cgtcatcacc
    11281 accgaggcgg ccgacgccgg acgaggc
//
LOCUS       DASYLX010000510         9903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2656253,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000510 DASYLX010000000
VERSION     DASYLX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656253"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2526
                     /locus_tag="VGP27_12390"
     CDS             <1..2526
                     /locus_tag="VGP27_12390"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852133.1"
                     /translation="AVVAEKTGYPAEMLNLDLDLEGDLGIDSIKRVEILAAIQEQAAD
                     MPAVDPATIGSLKTLGSIVDHIQGLLEGAVAPVATTTAPVETPLPPNLGRYRLKRIDA
                     PSTGLAQPGLLNDEVHVTGGGELDEALAAELRVRGVDARATARLPVGAVACVYLGGLR
                     EMANEDAAVAVNREAFAVARQLAQRLETGRGLFVTVQDTGGSFGLGPLDARRAWLAGL
                     PALVKTASQEWPLASLKSIDLERGAREAHALAVALADELLQGGAEIEVALPAQGGRFT
                     LRSVNEPLAPGQAVIGAGEVVLVSGGARGVTAACLHTWAGDCSARFVLLGRTAIAQEP
                     AICAGIDDEAGVKRVLLEAARAGGHMPTPAELGARVQEILANREVRRTLTAIERAGGQ
                     ARYEPVDVCDGPALAPILDRVRAEWGPIAGLVHAAGVLADKKISEKTNEQFDRVFDTK
                     VAGLRALLAATADDPLKLLCVFSSVAARCGNYGQADYAMANEVLAKVAWSEQRRRPGL
                     LVKSLGWGPWQGGMVTPALERRFTALGVPLIPLAVGARMFADEMCASQRDEVELVLGG
                     EPRSEALLSEGAENKVHELELRVCRESHQYLEGHAIGGTPVVPVVLAAEWLSRAACSF
                     RPGRALVALHDITVLKGIRLNSFNDGGDRFTIQAGQWPNANGAMLRLELRGSGGTLHY
                     SACAEMAEAHRAPENGVPDLPLDVWAGEPLYREMLFHRGAFEVIEELCGISDQGVSGQ
                     VRGVLRAGWPREPWQLDVAALDGALQLAVLYGRRMLCGPNLPTAIDELRTYGALPGSS
                     KIQASAYRRSVSSAAVTTDIVLTDEHGQRLAELRGVHCHALPR"
     gene            2570..7393
                     /locus_tag="VGP27_12395"
     CDS             2570..7393
                     /locus_tag="VGP27_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7852134.1"
                     /translation="MNAEHMAPAPALPAILNEDWTNEDWTVVATPTTPIAPPAMPVVA
                     ADTPVSEVLTGYRAHLAQLAQGLQDFLLAQQSLHQRFLSLREAAYDTLLQAAAVTGSP
                     LTTLPEPQAGLVVPVSPADPGPTSSDRLVTPTSDASDGNPGPTGASFSRQQLEIHAGG
                     RVSEIFGPKFATQDGYHRQVRLPNPPLLLADRVVNLQAEPLTQGKGTIWTETDVCHDS
                     WYLHDGYMPAGVLVETGQAILMLISYGGVDLLNRGERVYRLLGCELTYHGDLPMAGDV
                     LHCATHLDSHVVQRDTQLTFFRHECHNGDRPQLSVRNAHAGLFTDAELASSIGCLWSP
                     EEQEIVVQPRLDPPAVATRCTSFDRGQIEAFASGDTFACFGPGFERAQTHTRSPRILG
                     GRMLLQDRVTCLEHQGGPWGRGYLRAEFDVQPDLWFFASHFKNDPCMPGTLMFDACLQ
                     ALALFLASRGYSLDRDGWRFQPVPEIAYQLEWRGRVTPTSRLLVTEVFVEEVIAGPRP
                     TVYADLLCSVDGVKAFHARRVALELVPGWPLQSMPELLAEARSDPRPVAVVDGFRFDH
                     ASLLACAWGKPSDMFGPMYSRFDGPSRVPRVPGPPFHFMNRIKEVQGPIGVMKPGAKV
                     WADYDIPADAWYFDAIGSRTVPFAVLAEAALQTCGWLSYYVGSALTVEQELGIRNLDG
                     TGTLHRELLPDSGTLTTQVELVAVSLTDSVIIETFRVRCLLGDTPVYDLKTVFGYFPP
                     AALASQVGLPRNDDHQELLNRSSDYLVDLTARPQPHFAVERAQLAQPMLLMIDRVDGF
                     WPGAGEAGLGQLRAVKDIDPGQWFFKAHFFQDPVQPGSLGITAMIQALQFYMLETRMD
                     EGIPHPRFECLATQREMSWKYRGQVLPHHRKVHTTLEVTQIGRDERGAFATCTASLWA
                     DGQRIYEAVGLGMRIVPGRVEDDRRTTSDEIALDPARDTWLGDHCPTFTVPALPMMSM
                     VDLLAQASTGARPVVAIRDVRVSGWCLVDRPRRLKGECSGEHVRLLDEDGCEVARARV
                     IHGGYGEKPTALPPIEGPEQPLPYQTGALFHGPAFQVLEKLVLGRGASSSLLRAHSGV
                     PVGLLNPGLLDGTLHGIPHADLHSWNDVYSVDKVAYPAFIPKIEFFGPTPNTGLVRCE
                     VRERPFLGTPDYPSFAMQLISEDGVWCQIELVESCFPKGPLVAAEPIERLAFMRDHMP
                     VDGLRLSRAGGAATLLDDADVAGIDWLPGTVETIYGTSETEEIARREHVAAAHRLHPG
                     RALAQLPLTRFDLPVFRTGSHVRVEGDGRGQLDIALVRDYWTHWFGRGPWPVEDLVYG
                     LIERFLGRVVLEAPDAYQALRGHSALYLANHQTGIESLIFSVVASGLNQVPTVTLAKI
                     EHRESWLGRLIRHCFEFPGVADPKLMTFFDRDDKESLPRIVAELAAEMIGPGRSVMVH
                     VEGTRSLSYLPPIRKMSGGFIDMALAVGAPIVPVRFIGGLPAEPVEKRLEFPSGMGRQ
                     DIHLGCPITPEELAPLHYGARKQRVIEAINALGVPNEREQPLPGDSEFAGRVWAWQRK
                     TGVSHEHATLHQVLAELPEPGDPIRRLLCARNAAELADPGPEGQWLAELGRRLLG"
     gene            7409..8119
                     /locus_tag="VGP27_12400"
     CDS             7409..8119
                     /locus_tag="VGP27_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEV7852135.1"
                     /translation="MWATILVMAAAVSLEPIRIGLTVMMLNRPRPVRQLFAFLCGGFV
                     MGLAVGLVVLFVLRHRLLSGATYFTLPIVQIAIGLLALLFAAALATSLGARHFQRTAV
                     DSASEVVVGGSTTARPGENLSRRARRLRQGDSLWAAGVVGLGIALPSVDYMAALAVIL
                     ASGAAPAAQVGALLTFNVVAFALVEIPLIGYLAAPSRTRALMAALHDWIQTRRRRDVA
                     AVLAAAGTVMIAIGVAGL"
     gene            complement(8321..8461)
                     /locus_tag="VGP27_12405"
     CDS             complement(8321..8461)
                     /locus_tag="VGP27_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852136.1"
                     /translation="MNRQLEAKTRALAGWKGYTTAQHNTTSGPQTTPATQSSEGTTSA
                     AS"
     gene            8580..9848
                     /locus_tag="VGP27_12410"
                     /pseudo
     CDS             8580..9848
                     /locus_tag="VGP27_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
BASE COUNT         1487 a         3345 c         3449 g         1622 t
ORIGIN      
        1 gccgtggtgg ccgaaaagac cggctatccc gccgagatgc tcaacctcga cctggacctc
       61 gaaggcgacc tcgggatcga ctcgatcaaa cgggtcgaaa tcctggccgc catccaagag
      121 caggccgctg acatgcccgc agtcgacccc gccacgatcg ggtcactgaa gacgctcggc
      181 tccatcgtcg accacataca aggcctgctt gagggtgccg tggctccggt tgcgacgacg
      241 acggcaccgg tcgaaacacc cctccccccg aacctgggcc gttaccggct gaagcgtatt
      301 gacgcgccgt cgaccggcct cgcccagccc ggcctgctga atgacgaggt tcacgtcacg
      361 ggcggtggcg agttggacga ggcgctggcg gccgagctgc gcgtccgcgg cgtcgatgcc
      421 cgcgccaccg cgcggctgcc cgtgggtgcg gttgcctgcg tctatttggg cggcctgcgc
      481 gagatggcga acgaggacgc ggccgtcgcc gtcaaccgcg aagcattcgc ggtggcccgg
      541 cagctcgccc aacggctgga gacgggtcgc ggcctgttcg tcacggtcca ggacaccggc
      601 ggctcgttcg ggctcggtcc gctggacgcc cggcgcgcct ggctggccgg cctgcccgcg
      661 ctggtcaaga cggccagtca ggagtggccg ctcgcctcgc tcaagtcgat cgacctggag
      721 cgcggcgccc gcgaagccca cgcactcgcc gttgcgctgg cggacgaact gctgcagggt
      781 ggcgccgaga tcgaggtggc cctgcccgcg cagggcggcc gcttcacact gcgaagcgtc
      841 aacgagcccc tagcgccagg ccaagcggtg atcggcgcgg gcgaggtggt gctggtctcg
      901 ggaggggccc gcggagtgac cgcggcgtgc ctgcacacgt gggcgggcga ttgctcggcc
      961 cgcttcgtgc tgctgggccg gaccgcaatc gcccaggagc cggccatctg tgccggcatc
     1021 gacgacgagg ccggggtgaa gcgggtgctg ctggaggcgg cgcgggcggg cggccatatg
     1081 ccgactcccg cggaactggg cgcccgcgtc caagagattc tggcgaaccg cgaagtgcgc
     1141 cgtacattga ccgcgatcga gcgagccggc ggccaggcgc gctatgagcc ggtcgacgtg
     1201 tgcgacggcc cagcactggc accgatactc gaccgggtcc gggcggagtg ggggccgatc
     1261 gcgggtctgg tccacgccgc cggcgtgctg gccgataaga agatctccga gaagaccaac
     1321 gagcagttcg accgcgtctt cgacaccaag gtcgccggcc tgcgtgccct gctcgccgcg
     1381 accgccgatg acccactgaa actgctctgc gtgttctcct ccgtcgcggc gcggtgcggc
     1441 aactatggcc aggcggacta cgcgatggcg aacgaggtgc tggcgaaggt cgcctggagc
     1501 gagcagcgcc gccggcccgg gttgctggtg aagtcgctgg gctggggccc ctggcagggc
     1561 ggcatggtga cgcctgcgct cgagcggcgg ttcacggcgc tgggcgtgcc cctgatcccg
     1621 ttggcggtgg gcgcgcggat gtttgcggac gagatgtgcg ccagccagcg cgacgaagtg
     1681 gagctggtgc tgggcggcga gccccgctcg gaggcgctgc tgtcggaggg cgccgagaac
     1741 aaggtccacg agctcgaact gcgggtgtgc cgcgagagcc atcagtacct ggagggccac
     1801 gcaatcggcg gcacaccggt ggtgccggtg gtgctggcgg ccgagtggct gtcgcgcgcc
     1861 gcgtgcagtt tccggcccgg ccgggcgctg gtcgccctgc acgacatcac ggtcctgaag
     1921 ggcatccgct tgaacagctt caacgacggc ggcgaccgct tcaccatcca ggctggacag
     1981 tggcccaacg cgaacggcgc gatgctgcgc ctggaactgc gcggttcggg gggcaccctg
     2041 cactacagtg cctgcgccga gatggcggag gctcaccgcg cgccggagaa cggagttccg
     2101 gaccttcccc tcgacgtctg ggccggcgag ccgttgtacc gcgagatgct gttccatcgc
     2161 ggcgcgttcg aggtgatcga ggagctgtgc ggcatctccg atcagggggt cagcggccaa
     2221 gtccggggtg tgctgcgggc cggctggccg cgcgagccct ggcagctgga cgtggcggcc
     2281 ctggacggcg ccctgcaact cgccgtgctc tatggccggc gcatgctgtg cggccccaac
     2341 ctgccgaccg caatcgatga gctgcggacg tacggagcgc tgccgggcag cagcaagatc
     2401 caggcctcgg cctatcgccg cagtgtgtcg agcgccgcgg tgacgaccga catcgtcttg
     2461 acggacgagc acggccagcg cctggcggag ttgcgcggcg tccattgtca tgccctaccc
     2521 cggtgagctc gcgccgaccg cctaagaagg agccactgag gagccatcca tgaacgcaga
     2581 acatatggcc ccggctcccg cgctgccggc gatcctgaac gaggactgga ccaacgagga
     2641 ctggaccgtt gtggctacgc ccacgacgcc catcgcaccg cccgcgatgc ccgtcgttgc
     2701 ggccgacaca ccagtctccg aggtcctgac cggctatcgg gcccacttgg cgcaactcgc
     2761 ccagggcctg caggactttt tgcttgccca gcagtcgctg caccagcgtt tcctctccct
     2821 gcgcgaggcg gcgtacgaca cgctgcttca agccgccgcg gtgaccggat cgcccctgac
     2881 gacgctgccg gagccgcagg ccgggctggt cgtgccagtg tcgccggcgg atcctgggcc
     2941 gacatcttcc gatcggctgg tcacgccgac gagcgacgct tcggacggga accccggccc
     3001 gaccggggcg tccttcagtc gtcagcaatt ggagatccac gcaggcggcc gggtctcgga
     3061 gatcttcggc ccgaagttcg ccactcagga cggctaccac cgccaggtgc gactgccgaa
     3121 tccgccgctg ctgctggcgg accgcgtcgt caacctgcag gccgagcccc tgacgcaggg
     3181 caaaggcacg atctggaccg agacggacgt gtgccacgac tcctggtacc tgcacgacgg
     3241 ctacatgccg gcgggcgtgt tggtcgagac cggtcaggcc atcttgatgc tcatttcgta
     3301 cgggggcgtc gacctgctga accgtgggga gcgcgtctac cggctgttgg gctgcgagct
     3361 gacctaccac ggcgatctgc cgatggccgg cgacgtgctg cactgcgcca ctcacctcga
     3421 cagccacgtc gtgcagcgcg acacgcagct gacattcttc cgccacgaat gtcacaacgg
     3481 cgatcggccg cagctctcgg tccgcaacgc ccatgccggg ctcttcaccg atgccgaatt
     3541 ggccagctcc atcggctgcc tgtggagccc tgaggagcag gagatcgtgg tgcagccgcg
     3601 gctggacccg cccgccgtgg ccacccgatg caccagcttc gaccgtgggc agatcgaggc
     3661 gttcgccagc ggcgacacgt tcgcgtgctt cggtccgggg ttcgagcgcg cccagactca
     3721 cacccgcagc ccgcgcatcc tcggtggccg gatgctgctg caggaccgcg tcacctgcct
     3781 tgagcaccag ggcggcccct ggggtcgcgg ctacctgcgt gcggagttcg acgtccagcc
     3841 ggacctctgg ttcttcgcca gccacttcaa aaacgacccc tgtatgccgg gcaccctcat
     3901 gttcgacgcc tgtctgcagg cgctagccct attcctggcc agccgtggct acagccttga
     3961 ccgcgacggc tggcgcttcc agcccgtgcc cgagatcgcc taccagctcg agtggcgtgg
     4021 ccgggtcacc ccaacctcgc ggctcctcgt cacagaggtg ttcgtcgagg aggtgatcgc
     4081 ggggccgagg ccgaccgtct acgcggacct gctctgttcg gtcgacgggg tgaaggcgtt
     4141 ccatgcccgc cgtgtcgcgc tggaactggt gccgggctgg cctctgcagt cgatgccgga
     4201 actgcttgcc gaggcccgct ccgacccgcg accggtggcc gtggtcgacg gcttccgctt
     4261 cgaccacgcg agcctgctgg cgtgtgcctg gggcaagccc tcggacatgt tcggcccgat
     4321 gtacagccgc ttcgacgggc cgagccgggt gccgcgcgtg cccggcccgc cgttccattt
     4381 catgaaccgc atcaaagagg tgcagggccc gatcggcgtg atgaagccgg gcgcgaaagt
     4441 gtgggccgat tacgacatcc ccgcggacgc ttggtacttc gacgcgatcg gcagccgcac
     4501 cgtgccgttc gcggtgctgg cggaggcggc gctgcagacc tgcggctggc tctcctacta
     4561 cgtcggctcc gcgctcaccg tcgagcagga gctgggcatc cgcaacctcg acggcacagg
     4621 cacgttgcac cgcgagctgc tgccggacag cggcacgctc accacccaag tggaactggt
     4681 cgcagtatcg ctcaccgact cggtgatcat cgagacgttc cgggtgcgct gcctgctcgg
     4741 cgacaccccg gtctacgacc tgaaaactgt gttcggttac ttcccgcccg cggcgctggc
     4801 aagccaggtg ggcttaccca gaaatgacga ccaccaggag ctgctgaacc gcagcagtga
     4861 ctacctggtt gacctgaccg cgaggccgca gccgcatttc gccgtcgagc gggctcagct
     4921 cgcccagccg atgctgctga tgatcgaccg ggtcgacggc ttctggcccg gcgccggcga
     4981 agccggcctc ggccagctcc gcgccgtcaa ggacatcgat cccggccagt ggttcttcaa
     5041 agcccatttc ttccaggacc cggtgcagcc cggctcgctc ggcatcaccg cgatgatcca
     5101 ggcgcttcag ttctacatgt tggaaacgcg gatggacgag ggcattccgc atccgcgctt
     5161 cgagtgcctg gcgacccagc gggagatgtc gtggaagtac cgcggccagg tgctgccgca
     5221 tcaccggaag gtgcacacca cgttggaggt aacgcagatc ggccgcgacg agcgcggcgc
     5281 cttcgcgacg tgcaccgcca gcctgtgggc ggacggccag cggatctatg aggccgttgg
     5341 cctggggatg cgtatcgtcc cgggccgggt cgaggacgat cgcaggacga cgagcgacga
     5401 gatcgccctc gatccggccc gtgacacctg gcttggcgac cactgcccga ccttcaccgt
     5461 gccggctcta ccgatgatga gcatggtcga cctgctggcg caggcctcaa ccggcgccag
     5521 gccggtggtg gcgatccgcg acgtgcgggt gagcggctgg tgcctggtcg atcggccgcg
     5581 gcgtctgaag ggcgagtgca gcggtgagca cgtgcgactg ctcgacgagg acgggtgcga
     5641 ggtggcgagg gcccgggtga tccacggcgg gtatggcgag aaaccgacag cactcccgcc
     5701 gatcgagggg ccggagcagc cgctgccgta ccagactggc gcgctgttcc acgggccggc
     5761 attccaggtg ctggaaaagc tggtgcttgg gcggggtgcg tcgtcgagcc tgctgcgagc
     5821 acatagcggc gtgcccgtcg gcctgctgaa cccggggctg ctcgatggca ctctgcacgg
     5881 catcccgcac gccgatctgc acagctggaa cgacgtctac tccgtcgaca aggtggcgta
     5941 tccggcgttc attcccaaga tcgagttctt cggcccgacg ccgaacaccg gcttggtgcg
     6001 gtgcgaggtc cgcgagcggc cgttcctggg cacaccggac tacccatcgt tcgcgatgca
     6061 gttgatcagt gaggatgggg tgtggtgcca gatcgaactg gtcgagtcat gcttcccgaa
     6121 gggcccgctg gtcgcggccg agccgatcga gcgcctcgcc ttcatgcgcg accatatgcc
     6181 ggtggatgga ctgcgcctgt cccgtgccgg gggcgccgcc acgctcctcg atgatgccga
     6241 cgtagcgggt atcgactggc tgcccggcac cgtcgagacc atctacggca ccagtgagac
     6301 cgaggagatc gcgcggcgcg aacacgtcgc cgcggcccat cgcctgcacc ccggccgcgc
     6361 gctggcgcag ctgccgctga cccgcttcga cctcccggtg ttccggacgg gtagccacgt
     6421 gcgcgtcgag ggagatggac gtggccagct cgacatcgca ctggtccgcg actattggac
     6481 ccactggttc gggcgtgggc cctggcccgt cgaagacctc gtctacgggc tgatcgaacg
     6541 cttcctgggc cgtgtcgtgc tcgaggcccc agacgcctat caggcgttgc gcggccacag
     6601 cgcgctgtac ctcgcgaacc accagaccgg catcgagtcg ctgatcttct cggtcgtggc
     6661 ctccggcctg aaccaggtgc ccacggtgac gctcgccaag atcgagcacc gcgagagttg
     6721 gctgggccgg ttgatccgcc actgcttcga gttcccgggc gtggccgacc ccaagctgat
     6781 gacgttcttc gaccgcgacg acaaggagag cctgccccgg atcgttgcgg agctggccgc
     6841 agagatgatc ggcccgggcc gctcggtgat ggtgcacgtg gagggcaccc ggtcgctctc
     6901 gtatctgccg ccgatccgga agatgagcgg gggcttcatt gacatggctc tcgcggtggg
     6961 tgcgccaatc gtgccagtgc ggttcatcgg cggcctgccc gccgagccgg tcgagaagcg
     7021 gctggagttc ccatccggaa tgggccgcca ggacattcat ctcggctgtc cgattacccc
     7081 tgaggagctc gcaccgcttc actatggcgc gcgaaagcaa cgggtgatcg aagcgatcaa
     7141 cgcgctgggc gtgccgaacg agcgggagca gccgctaccg ggcgattcgg agttcgccgg
     7201 ccgcgtgtgg gcctggcagc gcaagaccgg cgtctcgcac gaacacgcga cgctgcacca
     7261 ggtgctggcc gagctaccgg agccgggcga ccccatccgc aggctgctct gtgcgcgcaa
     7321 cgcagcagaa ctcgcggatc ccggcccgga aggccagtgg ctcgccgaac tcggccggcg
     7381 actgctgggc tgagggtgtt ggtcagacat gtgggcaacc attctggtga tggccgccgc
     7441 ggtgagcctc gaacccatcc ggatcgggtt gaccgtgatg atgctgaaca gaccgcggcc
     7501 ggtgcggcag ctgttcgcgt tcctgtgcgg tggtttcgtc atgggcctgg ccgtcggcct
     7561 cgtagtgctc ttcgtccttc ggcacaggct gctgtcgggc gccacgtatt tcaccctgcc
     7621 catagttcaa atcgcgatcg gcctgctcgc cctgctgttc gccgccgcgc tggccacgag
     7681 tctgggggcg cgccatttcc agcgcaccgc ggtcgacagc gcttctgagg tggtcgtcgg
     7741 agggtcgacg acagcacgtc ccggcgagaa cctgagcagg cgtgcgcgcc gtcttcggca
     7801 gggagattcc ttgtgggccg ccggagtggt cggcttgggg atcgcgttgc cttccgtcga
     7861 ctacatggcc gcgctggctg tcattctcgc ctcaggcgct gcacccgcgg cacaggtcgg
     7921 ggcgcttctc acgttcaatg tcgtggcgtt cgcgctggtg gagatcccgc tgatcggcta
     7981 cctggcggcg ccatccagaa cgcgcgcgct gatggcagcg ctgcacgatt ggatccagac
     8041 tcgccgccgc cgggatgtgg cggccgtgct ggcagcggcg ggaacagtca tgatcgcgat
     8101 cggggtggcg ggtctttgag gtgacggcag gcaggcgggc ctacctggcg gccggcggcg
     8161 caggtcaaac accgtttaca ccaaaccggg ctgcgtgcaa tgcgacgccg ccctgcgcgc
     8221 ctggacaacc tgacttttat cactgtgatg ggttctgcgt cgttgtaggg ctgcgatctg
     8281 cgggtttgtc tggcggctgg tgcggatttg tgcactagga ctaggacgcc gctgatgtag
     8341 tgccctcgct ggattgggtt gccggtgtgg tttgtgggcc agaagtggtg ttgtgctgtg
     8401 ctgtggtgta gcccttccag cccgccagcg ctcgggtctt ggcctccaac tgccggttca
     8461 cgctcttggt ggcacctatc acacccgcca ccgacaagac caccgccggc gacggggatc
     8521 ccactgcgcg tcccggtgat gcggcagatc cgttggcgtg cagaccgttg gcgccacgcg
     8581 aactggtcgc gcaactgcgg gtcgcgtccg tgcagcacct ggtggccgtc gaggagttcc
     8641 gcgggcaccg ctatctcgac gagctgaact ccgagtgcga cgcgccgacg gatctgcctg
     8701 cgctccggca tgtttggacg cccgatcgtc tacctcaggc cactgccacg actcgcgggg
     8761 ccgtcgacgc aatcaccgct acggtcacgg ccgccgacac cctggtcatc atgttcacct
     8821 cggggagcag cggtccgccc aaaggcgtta tccattcaca cggcaacgca ttgggcgccg
     8881 tacagtccgg ccttgcggct cggtgcatcg acgcagacac tcgtctctac cttccgatgc
     8941 cgttcttctg ggtgggcggc ttcggcagcg gcatcctgtc ggcactgctg gcgggtgcaa
     9001 cactgatcac cgagcagatt ccgagccccg acaccacgct gcgcctgctg gaacgcgaac
     9061 gagtgacatt gtttcgcggc tggccagacc aggccgaagc cgttgcccgc caggcgacct
     9121 cgatcggcgc cgacctgtcc gccttgcggc cgggcagcct ggaggcgctg ctgccgcccg
     9181 ctcaacgggc agagcccggc gcgcgggcca agttgttcgg catgaccgag tcgttcgggc
     9241 cgtactgcgg ctacccggcc gacaccgata tgccgcggac ggcatggggc agctgcggaa
     9301 aaccgttcac cggcaccgag gtccgcgtcg tcgaccccga taccggcgag ccggtgccga
     9361 cgggggccgt cggcatgatc cagattcgcg ggccgaacac gctgcgcggc atctgtcgac
     9421 gcagccggga agaggtcttc accgcagacg gcttctaccg caccggcgac ctagcccacc
     9481 tcgatgacga cggcttcatg ttctaccacg gcagatccga cgacatgttc aaggtcagcg
     9541 gcgccaccgt ctacccctgc gaggtcgagc aggcgctgtg cggcatcgag ggtgtcgcaa
     9601 acgccttcgt caccgatgtg tctggcgcag tgggtgcggt cgtgatcgcc gacgagtccg
     9661 aggaacatct gcgggcggct gcgcgaaggc tgttgagccc gttcaaggtt ccgaccgtgt
     9721 ggctggtcgt cgactccgac gacgtcatcc ctcgcggcgg caccggcaag gtcgatgtcg
     9781 cggagctgag aaagatgctg accgctgaca ctcctctcgg cgagcagacg cgaactcgca
     9841 caaattgagc gctgagagtg cgagttcgcg tctgctcgcg gggtgaggtg agctgctcgc
     9901 ggg
//
LOCUS       DASYLX010000511         9376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2985943,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000511 DASYLX010000000
VERSION     DASYLX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985943"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VGP27_12415"
     CDS             <1..142
                     /locus_tag="VGP27_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HEV7852137.1"
                     /translation="AANPDIPEIDGVPCTGHDSGACIGLSEEQQDMGPQPVPRSTISS
                     SP"
     gene            172..795
                     /locus_tag="VGP27_12420"
     CDS             172..795
                     /locus_tag="VGP27_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852138.1"
                     /translation="MISSFFSAKSARSVGLLVRPNDAFTALIRCGSRSRSLRRATIVE
                     SLRVRTVIDVAGRRVRAPSADLGDWRIPRNRTVLVRISDVHVNPEVFPHSERFDPHRF
                     RGAKAVAPTWLASGGGPRRCIGADFAVAEMDVVLRTVLQNFRIQTDAAAEEKSHFQGI
                     AHTPKLGGCRADRCSGSRFRWRAPRPRSRTAGVNSLTRNTSSLAVAR"
     gene            690..1007
                     /locus_tag="VGP27_12425"
     CDS             690..1007
                     /locus_tag="VGP27_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852139.1"
                     /translation="MQRVAVSMESSKAAIEDGWRELINAQHQQLGGCQVTLALLDAMG
                     RFITEIGEQLSALRVEQRPGHVICLIMPEGMEDSSHECTWQTVEMLAKRPQPEQCGAR
                     TRW"
     gene            1169..2407
                     /locus_tag="VGP27_12430"
     CDS             1169..2407
                     /locus_tag="VGP27_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV7852140.1"
                     /translation="MTARFVGRVGGVLACAVVAACSASQPAAPSNTGPEELNYSRQMP
                     AITNGDLAARGTVASSSDQTPFDFSELPVGSTAHTMVYRSISGITGLPTVVSGAVFTP
                     PGDPPPGGWPVIGYAHGTVGVTPDCGPTADSRLLGDITPVAIQLKLGYAVAYTDYAGL
                     GKPDGSSQDAMTPHAYLEPKSAAFNLIDAVRAARTVVPQLSSRWVALGSSQGGETAWA
                     AAEYSADYGQGTDLLGAAALVPTADMLGLVQRAESSTLTNDQMFLYPNVIEGLASVDK
                     AIDPNDYLHGVLRDDKATLLACRPPASQRKDALAAKFAPADAEPSSPAAADRLSQRLS
                     SYALPQQPTKIPLLAIYGGSDQTVPPEWTEVAMGRACALGDTILRIRLQDQGHKLDPG
                     AALGQWIADRFAGVPAVGNC"
     gene            complement(2423..3421)
                     /locus_tag="VGP27_12435"
     CDS             complement(2423..3421)
                     /locus_tag="VGP27_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV7852141.1"
                     /translation="MSLPSGHAARLSRRSLLASIPLAAFTVVAAGGCQRSEPRQPDQP
                     HESRRFGLAATGIDDATVQRSQAAAQQLGKHLDVLTVYEAFVWERSLPTTFLDAAAAA
                     GCTPELTWEPWDPRAGMHQVAYTAGQIAGGRYDNYVRTWANQAASYDKPFRLRFAHEM
                     NGNWYPWAVGSPGGSSQDYVAAYRRVRSIFDDAGAKRVEWVWCPNVIIDGNAEAISPC
                     YPGDSFVDILGVDGYNFGDRPGHQWIQPAELFGTTLALVAQLAPGKPVWINEVGCGDQ
                     GGDKSRWISDFVSWLAGTDVRGLTWFEVDGNPDAPDWRLTSSAVTTAAAKAVLASW"
     gene            complement(3489..5732)
                     /locus_tag="VGP27_12440"
     CDS             complement(3489..5732)
                     /locus_tag="VGP27_12440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010844524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7852142.1"
                     /translation="MTKYWEEPTVRYRPGPTSATAQTARMQLPRTSRTASIPAPAHHR
                     VHRHPATVVVPTQPSTAKAKVEPKPEAKKSENRFFNDVEGLRGIAVALVVLFHAGVPH
                     MAGGFVGVDVFFVISGFLITGLLLREFERNGRVSFRGFYARRARRIIPPAAVTIIATS
                     IAVWFLMPLLSVFRQALDLLAATMNIANWRFIAAGKDYLAGASDDSVATHFWSLSIEE
                     QFYFIWPVLLVALAVLAKRMRWSIRMVVGWGIAVVTAASLLASLHFTASDPTLSYMAT
                     HTRAWQFGIGGVVAVAGPLLTGLITRLSVRVAMWVLGWAGLAAVLVATVAYDHTTPYP
                     GVAALLPTLGAAAIIVAGQVATSSRPAVGWFLGARPLRWLGKVSYGWYLWHWPALVLF
                     KSYTHNTGWPTLVGVTMAALALAWASTALLERPIMSSGELKRNLQASLSVGFTGTVVA
                     AAVTMSLGVLAVNLASGATSSNASVSFQSVFGADTGAKSGPVTPNPFKAFDDRPGRDE
                     CLVPLGKREQPSTCVDGPAGGVPAVLFGDSHAQQWLPVVQVIAKENNWRLNQFTKAAC
                     PVAALQPRDGRTDPFTKSDCLGWRADSVKAIVALKPKYIVISSLSTYVPDYQEFKTAW
                     DQTLNQLRATGAKLIYLRDTPYPNKNMPECISGATDNWSACDFNLNNTPRTEPIVTDQ
                     VRGQNLDIPVLDLNAYLCEDNKCRAVRNGTLLYRDDSHLTATAVKALLPAVQAQVRDK
                     GIDLSGR"
     gene            complement(5900..7900)
                     /locus_tag="VGP27_12445"
     CDS             complement(5900..7900)
                     /locus_tag="VGP27_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014359067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase catalytic subunit"
                     /protein_id="HEV7852143.1"
                     /translation="MPLAQMPRTSGSRTLGRNDLNRSDPRRHVHRPDPRRHVRRHDHR
                     GADGTWTGRTKVLHEPLTERLALHEPATERIVRKDLAALETYQLTEQAVEAEAEGLVA
                     PPADNEVYSYFGAQMRWVQILLLAASVLAGWSLLHFAIASIAVLWPMLIVLGLNVVGT
                     ILSSLTSFNTRRVSAKTHRKLVEGWVPSGPAPSIDVFLPTYGEDVAVLRNTYTHVKAM
                     EWAARVDVHVLDDGDRGEVGELAAEFGFDYVVRPNRGHLKKAGNLQYAFSRTSGDHIV
                     ILDADFCPRPDFLQHLVPYMDDPAVGIVQSPQYFDSGENLNWIQRTAGSTQELFYRWI
                     LPSRDRVNAAICVGTCALYRRKALDATGGFAQIEHSEDIHTGLHLMQAGFQTRYVPIV
                     VSRGLCPGDMAGFLNQQYRWCNGSLVKLHNAKLDGSRVKMTLRQRLCFWAGLFYYITT
                     AVNVLALYLPGMIMAAFFPTQVQPAQFVPFLVGLWVYLVVIPVVSKSRWRFEVLRLQM
                     AYSYAHLIAIVHKLRGRSAGWVPTGAMDRPNPLARSISRLGAVAIMASLVPFWAVVIY
                     DIHAYGVRQYWLMAMFVGIYTYLALPLLVEFIKILWPSFGKLLGSRTDGASAPATQSG
                     PKPRRRNPHRISAYEAVCYTLALVLAAAVASGWFDLMIPWTS"
     gene            complement(8215..8547)
                     /locus_tag="VGP27_12450"
     CDS             complement(8215..8547)
                     /locus_tag="VGP27_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852144.1"
                     /translation="MEPQSPCEPEHDTTTQEGTDDLMMPLTPGAQIDELRRLLRESEQ
                     RRQAAEHKLADRERQLGTASQTIAAQDHRLGSMTGELARLRGTAAATVVNAYALDFPH
                     FFTSNQKA"
     gene            complement(9086..9349)
                     /locus_tag="VGP27_12455"
                     /pseudo
     CDS             complement(9086..9349)
                     /locus_tag="VGP27_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT         1715 a         2943 c         3071 g         1647 t
ORIGIN      
        1 cgcggccaac cccgacatcc cggagatcga cggcgtcccg tgcaccggcc atgacagcgg
       61 ggcctgcatc ggcctgtcgg aggagcagca ggacatgggc ccgcagccgg tgccgcgctc
      121 gaccatcagt tccagcccgt aacacttcgg aatgcgaacc ccaaagcccc gatgatcagt
      181 tcgttcttct cggcgaagag cgcgaggagc gtcggtctgc tagtgcgccc gaacgacgca
      241 ttcaccgcgc tcatacggtg cggaagccga agcagatcgc ttcgtcgggc cacaatcgtc
      301 gagtcgctgc gggtgcgcac cgtcatcgat gtggccggcc gccgggtcag ggcgccgagc
      361 gccgacctcg gcgactggcg gattccgcgg aatcgcacgg tgctcgtgag gatctccgat
      421 gtgcatgtga atccggaagt cttcccgcat tcagaacggt tcgatccgca ccgcttccgc
      481 ggcgccaagg cggtggcacc gacctggctc gcgtccggtg gcggaccgcg tcgatgcatc
      541 ggcgccgatt tcgcggtcgc cgagatggat gttgtgctgc gaacggtgtt gcagaacttc
      601 cggatccaga cggatgccgc cgccgaggag aagtcgcatt ttcaaggaat cgcccacact
      661 ccgaaactcg gcggctgtcg ggcggacaga tgcagcgggt cgcggtttcg atggagagct
      721 ccaaggccgc gatcgaggac ggctggcgtg aactcattaa cgcgcaacac cagcagcttg
      781 gcggttgcca ggtgaccctt gccctgctcg acgccatggg gcgattcatc accgagatcg
      841 gcgaacagtt gtcagcgctg cgcgtcgagc agcggccggg ccacgtcatc tgcctgatca
      901 tgcccgaggg gatggaggac tctagtcacg aatgcacttg gcagacagtg gaaatgctgg
      961 ccaagcggcc acaaccggag caatgcggcg cgcgcacgag atggtgagcg aaacgcgcgc
     1021 cgggaggtcc gctgcgttct cggacgaaga ccgccaagcg gcgatgggag gtcagctacc
     1081 cccgcgtggg cagaatgcaa gcgactggaa aggcctctga agggtgtcgc ctatgccatc
     1141 ctcgtgatca aggatcaagg ggacacacat gaccgcgagg ttcgttgggc gagtgggcgg
     1201 cgtgctcgca tgcgccgtgg ttgccgcgtg cagcgcgtcg caacccgccg ccccctccaa
     1261 cacagggccg gaggaactga actactcccg acagatgcca gccatcacca acggtgactt
     1321 ggccgcccgc ggcaccgtgg cgagctcatc agatcagacg ccgttcgatt tcagcgagct
     1381 gcctgtcggc tcaacggcac acacgatggt gtaccgatcg atatccggca tcactggatt
     1441 gcccaccgtg gtttccggtg cggtattcac accgccaggc gacccgccgc ccggagggtg
     1501 gcctgtcatc ggctacgccc atggaaccgt tggggtaacc cccgactgtg gcccaaccgc
     1561 ggattcccgc ctgctcggcg acatcactcc ggtggcgata cagctcaagc tcggctacgc
     1621 ggtggcctac accgactatg cgggtctggg caagcccgac gggtcaagcc aggacgcgat
     1681 gacaccacat gcttacctag agccgaagtc ggcggcattc aacctcatcg acgcggtgcg
     1741 agccgcccgc acggtggtgc cgcagctgtc gtcgcggtgg gtggcgctgg gatcctcgca
     1801 gggcggcgag accgcttggg cggccgccga atattctgcg gattacggtc agggcaccga
     1861 tctgcttggc gccgcggcgc tggttcccac ggcagacatg ctcgggctgg tgcagcgggc
     1921 cgagtcatcg acgttgacca acgaccagat gttcctgtac ccgaacgtga tcgagggttt
     1981 ggcatcggtc gacaaggcaa tcgatccgaa cgactacctg cacggcgttc tacgcgacga
     2041 taaagcgacg ttgcttgcct gccgaccgcc ggcgtcccaa cgcaaggatg ccctggccgc
     2101 caagttcgct ccggccgacg ccgagccgtc gtcgccggcc gcggccgacc ggctgagcca
     2161 gcggctcagc tcctacgcgc tgccccaaca gccaaccaaa atcccgctgt tggcgatcta
     2221 cggtggatcg gatcaaaccg ttccgccgga atggaccgag gtcgccatgg gccgggcctg
     2281 cgcgttgggc gacaccattc tgcggatccg cctgcaagac caagggcaca aacttgatcc
     2341 cggtgcggcc ttgggccagt ggatcgccga tcgcttcgcc ggcgtccccg ccgtcggcaa
     2401 ctgctgaccg agatcagccg cgctaccagg acgccagtac ggccttggcg gcagcggtgg
     2461 ttacggccga actcgtgagc cgccagtcgg gtgcatcggg gttgccgtcg acctcgaacc
     2521 aggtcagccc gcggacgtcg gtgccggcga gccagctgac gaaatcggag atccagcgcg
     2581 acttgtcgcc gccttggtcg ccgcagccga cctcgttgat ccacaccggt ttgcccggcg
     2641 cgagttgcgc gacaagggcc aacgtggtgc cgaaaagctc agcaggctgg atccattgat
     2701 ggcctggccg atcgccgaag ttgtagccgt cgacgccgag gatgtcgacg aaactgtcgc
     2761 cgggatagca cggtgagatc gcctcggcat tgccatcgat gatgacgttc gggcaccaca
     2821 cccactcgac gcgcttggcc cccgcgtcgt caaaaatgct gcggactctg cggtaggccg
     2881 cgacgtagtc ttgcgacgag ccgcccggtg atcccaccgc ccaggggtac cagtttccgt
     2941 tcatctcgtg tgcgaaccgg agccggaacg gtttgtcata ggaggccgcc tggttggccc
     3001 aggtgcgcac atagttgtcg tatcgaccac ctgcgatctg cccggccgtg taagccacct
     3061 ggtgcatgcc cgcgcgggga tcccagggtt cccacgtcaa ctccggggtg cagccggcgg
     3121 ccgcggcggc atccagaaac gtggtgggca gcgacctctc ccagacgaat gcctcgtaga
     3181 cggtcaacac gtccaagtgc ttgcccaatt gctgcgcggc ggcctgtgac cgctgcaccg
     3241 tcgcgtcgtc gataccggtt gcggccagcc cgaaccggcg tgattcgtga ggttggtctg
     3301 gttggcgcgg ttctgacctt tggcatccgc cggcggccac cacggtgaac gccgccaggg
     3361 ggatcgatgc gagcaggctg cgacgggaca acctcgccgc gtgtccgctg ggcaacgaca
     3421 cctccgaccc tcatgtgttc ggcagagaca caccctacgg gacgcccgag ctttcgcccc
     3481 gccgaacgtc agcggccgct cagatcgatt cccttgtcac ggacctgggc ttggaccgcc
     3541 ggcagtagcg ccttgaccgc ggttgccgtc aggtgagaat cgtcgcggta gagcaacgtg
     3601 ccgttgcgca ctgcgcggca tttgttgtcc tcgcacaggt acgcgttgag gtcgagcacg
     3661 ggaatgtcga ggttctggcc ccggacctgg tctgtgacaa tgggttcggt gcggggggtg
     3721 ttgttcaggt tgaagtcgca cgcggaccag ttgtcggtgg cgcccgagat gcattccggc
     3781 atgttcttgt tcgggtatgg cgtatcgcgc aagtagatca acttcgcgcc cgtcgcccgc
     3841 agttggttca gcgtttgatc ccaggcggtc ttgaactcct gatagtcggg cacgtacgtg
     3901 gacagcgagc tgatcacgat gtacttgggc ttcagcgcga cgatcgcctt gacgctgtcc
     3961 gcacgccagc cgaggcagtc cgacttcgtg aacgggtcgg tgcggccgtc tctgggttgt
     4021 agggcggcga cagggcatgc cgccttggtg aattggttga gccgccagtt attttccttg
     4081 gcgatgacct gaaccaccgg cagccactgc tgtgcgtggc tgtcgccgaa gagcaccgca
     4141 ggcacgccgc ctgctgggcc atccacgcaa gtgctcggtt gttcgcgctt ccccagtgga
     4201 accaggcact cgtcgcggcc gggccgatcg tcgaaggcct tgaacgggtt gggcgtgacc
     4261 ggtccagatt tcgcaccggt gtcggcgccg aaaacggatt ggaagctcac cgacgcattg
     4321 gacgaagtcg ccccgctggc caggttgacc gccaagacgc ccaggctcat cgtcacagcg
     4381 gcggccacaa cggtgccggt gaagccgacc gacagtgagg cctgcagatt gcgcttcagt
     4441 tcgcctgacg acatgatcgg gcgttccagc agtgcggtgg aggcccatgc cagcgccagt
     4501 gccgccatgg tcacgcccac caacgtcggc cagccggtgt tgtgggtgta gctcttgaac
     4561 agcaccagcg cgggccagtg ccacagatac cagccgtagg agacctttcc cagccaccgc
     4621 aagggacgcg ccccgagaaa ccatccgacg gcgggcctgc tgctggtggc cacctgtccg
     4681 gccacaatga tcgcggcggc gccgagcgtc ggtaacaacg cggccacgcc cggataaggc
     4741 gtggtgtggt cgtaggcgac tgtcgccacc agtactgccg ccagtcccgc ccagccgagc
     4801 acccacatcg ccactcgcac ggacaatctc gtgatcaagc cggttagcag cgggcccgca
     4861 accgcgacga ctccgccgat gccgaattgc catgcgcgcg tgtgggtcgc catgtatgac
     4921 agggtggggt cgctggccgt gaagtgcagc gatgccaaca gggaggcggc ggtgaccacg
     4981 gcgattcccc agccgacgac catacggatg gaccatcgca tgcgcttggc cagcacagcc
     5041 agtgcgacca acagcacggg ccagatgaag tagaactgtt cctcgatgga aagcgaccag
     5101 aagtgcgtgg caacgctgtc gtccgacgcc ccggcgaggt agtccttgcc cgcggcgatg
     5161 aagcgccagt tggcgatatt catcgttgcg gccagcagat cgagtgcctg gcggaagacc
     5221 gagagcagcg gcatcaggaa ccaaacggcg atcgacgtcg cgatgatggt cactgccgcc
     5281 ggggggatga tgcggcgtgc acgccgcgcg tagaagcccc tgaaggacac ccgcccgttg
     5341 cgctcgaact cacgcagcag caggccggtg atcaggaagc ccgaaatgac gaagaacacg
     5401 tcgacgccca caaagccgcc agccatgtgc ggcacacccg catgaaacag caccaccagc
     5461 gcgacggcga tgcctcgcag gccttcgaca tcgttgaaga agcggttttc cgatttcttg
     5521 gcttcgggct tgggctcgac cttcgccttg gccgtggacg gctgtgtcgg gacgaccacg
     5581 gtggccggat gccggtgtac acggtggtgc gccggcgccg ggatactcgc ggtacgggac
     5641 gtccgcggca gctgcatcct cgccgtctgg gccgtcgcgc tggtcggccc ggggcgatac
     5701 ctcacggtcg gttcttccca gtacttagtc atgaggaaat cgtcgcgttt tcggagtacg
     5761 ggcgtaatcg ccggcaagcc agaatcgact gctgacaggg aagggttttc tatccccctg
     5821 gtaagggagg ccggcggttg ctcccgaacc tcctgtgcca cagagaattc ggttcggcgg
     5881 gcatcggcgg tagtcgaagt caggaggtcc agggaatcat caggtcgaac cacccggacg
     5941 cgacggctgc ggccagaacc agcgcgagtg tgtagcacac cgcctcgtaa gcggagatcc
     6001 gatgtggatt gcgccgcctt ggcttagggc cgctttgggt tgctggtgcc gaagcgccgt
     6061 ccgtccggga tcccaggagc ttgccgaagg atggccacag gatcttgatg aactcgacaa
     6121 gcaacggcag ggcgagatat gtgtagatac ccacgaacat cgccatcagc cagtactggc
     6181 gcacgccata ggcgtgtatg tcgtagatga ccacggccca gaacggcacg agcgatgcca
     6241 tgatcgcaac ggcgcccaac cggctgatgc tgcgggccag gggattgggg cgatccatcg
     6301 cgccggtggg tacccatccc gctgagcggc cccgcagttt gtgcacgatt gcgatgaggt
     6361 gggcgtagct ataggccatc tgcaggcgca ggacctcaaa ccgccagcgg ctcttggaca
     6421 ccaccggtat caccaccaga tagacccaga gtccgacgag gaagggcaca aactgtgctg
     6481 gctggacctg ggtcggaaaa aacgcggcca tgatcatgcc gggaaggtag agcgcgagga
     6541 cattgacagc ggtggtgatg taatagaaca accccgccca gaagcagagc cgttgccgca
     6601 aggtcatttt cactcgggag ccgtcaagct tggcgttgtg cagcttcacc agcgacccgt
     6661 tgcaccaccg gtactgctgg ttgagaaagc cggccatgtc gcctgggcac aagccgcgtg
     6721 agaccacgat aggcacgtag cgggtctgaa agcccgcctg catcagatgc agccccgtgt
     6781 gaatgtcttc gctgtgctcg atctgcgcga atccgccggt ggcgtcgagg gccttgcgac
     6841 ggtacagcgc gcaggtgccg acgcagatcg cagcgttgac ccggtcccgc gacggcagga
     6901 tccagcggta gaacaattct tgggtcgagc ccgccgtgcg ctgaatccag ttcaggttct
     6961 cgccgctgtc aaagtattgc gggctctgta cgatgccgac ggccgggtca tccatatacg
     7021 gaacgaggtg ctgcaggaaa tctggccgcg ggcagaaatc cgcgtccaga atcacgatgt
     7081 ggtctcccga cgtgcgggag aaggcgtatt gaaggttgcc ggctttcttc aggtgcccac
     7141 gattcggtcg cacgacgtag tcgaagccga actctgcggc cagctccccg acttcgccgc
     7201 gatcgccgtc gtcgagtacg tgaacgtcga ccctggccgc ccactccatc gccttgacat
     7261 gcgtgtacgt gttgcgcaga accgccacgt cttctccgta tgtgggaaga aagacatcga
     7321 tgctcggcgc aggacctgag ggaacccaac cctcgacaag cttgcggtgc gtcttcgctg
     7381 acacccgcct ggtgttgaag ctggtcagcg acgaaagtat ggtgccaacc acattcagcc
     7441 ccaatacaat caacatcggc cataagactg cgatcgacgc gatggcgaaa tgcagcaggc
     7501 tccatcccgc gagcacagag gcggccagca gtaggatctg cacccaccgc atctgcgcgc
     7561 cgaagtagga gtagacctcg ttgtcagccg gcggggcgac cagaccttct gcttcggctt
     7621 cgacggcctg ttcggtgagc tgataagttt ccagcgcggc cagatccttg cggacgatcc
     7681 gctctgtggc aggctcgtgc agcgctagcc gctccgtgag gggctcgtgc agcaccttgg
     7741 tgcggcctgt ccacgtcccg tcggcgccac gatgatcgtg ccgccggacg tgtcgacgcg
     7801 ggtctggccg gtgcacatgg cggcgcggat cggaccggtt caggtcattg cgcccgagtg
     7861 tgcggctacc ggacgtgcgg ggcatctgcg cgagcggcat ggcgccggcc gggaccgtag
     7921 cttccgtcgg gccagcacga aggctcgtcg tctcttcgca gtacgtagtc atagaaacat
     7981 cgtcgtgatc tgggagcgcg tccgtaatcg ccaggaagcc agagtccact tatgaagggg
     8041 aaggatttcc tatccgtctg gtgagcactt ggggttcgac tcggcctgga gcccagcaca
     8101 aaagtccccg gcagtagcgc cggggacttt gaactgctgt cgggcgtcga agggcagtgc
     8161 ttcgcccggt acgcagcgtg aggtagggct ctggctagaa cgcagcaagg ggcgtcaagc
     8221 cttctggtta ctcgtgaaga agtgcgggaa gtcgagcgcg taggcgttga ctacagtcgc
     8281 tgccgccgta ccccgcagcc gcgcgagttc accagtcatg ctgccgagac ggtgatcttg
     8341 ggcagcgatc gtctgggatg cggtgccgag ttggcgctcg cgatcggcga gcttgtgttc
     8401 ggcggcctgg cggcgctgct ccgactcgcg caaaagacgg cgcagttcat cgatttgtgc
     8461 gccgggtgtg agtggcatca tgaggtcatc agttccttct tgtgtcgtgg tgtcatgttc
     8521 tggctcgcag ggtgattgag gttccaccgt gattccgtca gcgtcaccag cagctcgaag
     8581 atgagtacgc ccacgaacgc gtgatgatgc ctcgaggtgg gaatcgcggt agcgtccgga
     8641 ctgccatgca cttcctcggc cgctccggtg ccggcgtctt cggctctctt gcatcaattc
     8701 cacgttcatg tcggatactc cttctcctat cgcctgcggt gtagacagtc cgccgctgta
     8761 ccgcgctgtg cagaggcagg catatgtggc caagtccaaa actcttgggc tagaaccgga
     8821 taggccgcta aacaagatcg gtagcaatac cgacagtgtg tcgtagccca gttcctttca
     8881 tgcgtaacga cgctccggcc accagttgag ttgcggttct ttccagtacg tggccatgag
     8941 ctaaatcatg atgatcaggt gatgattccg taatcgccgg aaagccagat tccggtctgg
     9001 aaccgaaagg gtttcctatc tgattggcaa gcaagccgga ggcgagaatg gaggcgactg
     9061 acaggcgccg ctctcagtat gactgtccgt cgacatggtc caacaccagc gcgaggggca
     9121 ggccaagcca cacgctgctt ccaccgcaga tagcacagca gcgcgcctga gcgtcggcga
     9181 gatcctcgcg gatgtagtcg ccgcggcggc cgccgatcca cgcctcaccg gattggagcc
     9241 acaatttcgt gagggctttg cgtcggaacg aaccctggca cgcattgccg cagtaaatct
     9301 tctggcttcg cttcgagagc tctgaaccac agccgcggca ctgcctcatg cgtttgacgc
     9361 tacgatcggc caccga
//
LOCUS       DASYLX010000512         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1340179,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000512 DASYLX010000000
VERSION     DASYLX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340179"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VGP27_12460"
     CDS             complement(<1..202)
                     /locus_tag="VGP27_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852145.1"
                     /translation="MIADDAFPVEPWHVRELALNVDRIAQTESLFALSNGHIGLRGNL
                     EEGEPHGLPGTYLNGYYETRPLP"
     gene            complement(202..951)
                     /locus_tag="VGP27_12465"
     CDS             complement(202..951)
                     /locus_tag="VGP27_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEV7852146.1"
                     /translation="MAAKLGLPEGVRACLFDLDGVLTDTASVHRRAWKEMFDAYLSAR
                     AQRTGDRFVPFDIDADYESYVDGKKREDGVRAFLESRGITLPEGGPDDDADAETVNGL
                     GNRKNELFQQRLQSDGVDVFDGSRRYLEAVSAAGLRVAVVSSSANTREVLDITGLARF
                     VEVRVDGVTMRDDGIAGKPAPDSFLHAAQLLETPPDHAAVFEDALAGVAAGHAGHFGV
                     VIGVDRVGHAEDLRHNGADVVVTDLAELLDS"
     gene            996..1481
                     /locus_tag="VGP27_12470"
     CDS             996..1481
                     /locus_tag="VGP27_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEV7852147.1"
                     /translation="MADLHPSVAALAPLLGVWAGQGTGVYPTIAPFDYLEEITFGHVG
                     KPFLSYAQRTRAVEDGRPLHGETGYLRVPDAGRVELVLAHPTGVTEVAEGTLSVRGTS
                     IEMELVATTIGRTASAKEVTALGRSIRVHGDELTYTVHMGAVGQPLQQHLTASLHRKT
                     A"
     gene            1478..>1624
                     /locus_tag="VGP27_12475"
     CDS             1478..>1624
                     /locus_tag="VGP27_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEV7852148.1"
                     /translation="MTPDDQREDGRIRVPTDLDAVTDVGAEDHSDIDPAAVERIWQAA
                     RHWYA"
BASE COUNT          268 a          560 c          532 g          264 t
ORIGIN      
        1 acggcagcgg ccgggtctcg tagtacccgt tcagatacgt gccgggcagc ccgtggggtt
       61 caccctcctc gagatttccc cgcagcccga tgtggccgtt ggacaacgcg aacagtgatt
      121 cggtctgggc gatcctgtcc acgttgagcg cgagctcgcg gacatgccac ggctctaccg
      181 ggaaggcgtc gtcggcgatc atcaggaatc cagaagctcc gccagatcgg tgacgacgac
      241 gtcggcgccg ttgtgccgca agtcctctgc gtgacctacc cggtccacgc cgatcaccac
      301 gccgaaatga ccggcatggc ccgcggccac cccggccagc gcgtcctcga agaccgccgc
      361 atgatccggg ggcgtctcga gcaactgtgc ggcgtgcaga aacgaatcgg gtgccggctt
      421 ccccgcgatg ccgtcgtcgc gcatggtcac accgtcgacg cggacctcga cgaacctggc
      481 caggccggtg atgtcgagca cctcgcgggt gttggcgctc gacgacacca ccgccacccg
      541 caggcccgca gccgaaaccg cctcgaggta gcggcgcgag ccgtcgaaca cgtcgacgcc
      601 gtcggactgc agcctctgct ggaacagttc gttcttcctg ttgcccagac cgttcacagt
      661 ctcggcgtcg gcgtcgtcat cggggccgcc ctcggggagg gtgatgccgc ggctctccaa
      721 gaaggcccgc acaccgtcct cccgcttttt gccgtcgacg tatgactcgt agtccgcgtc
      781 gatgtcgaag ggcacgaacc ggtctccggt gcgctgcgcc cgcgcgctca ggtaggcgtc
      841 gaacatttcc ttccaggccc tgcggtgcac gctggccgtg tcggtgagta cgccgtcgag
      901 gtcgaacaga caggcccgga ccccttcggg caatcccagc ttcgcggcca tgtgccgggt
      961 tatacccaag tcgggccatc catagactgg gccgcgtggc cgatcttcat ccttccgtcg
     1021 ctgcgcttgc gccgcttctg ggtgtttggg ctggtcaggg gacaggcgtg tacccgacca
     1081 tcgcaccgtt cgactacctc gaggaaatca cgttcggcca tgtcggcaaa ccgttcctca
     1141 gctacgccca gcggacgagg gccgtcgagg acggccgccc cctgcacggc gagaccggct
     1201 atctacgggt gcccgacgcc ggccgggtgg agctggtgct ggcgcatccc accggcgtca
     1261 ccgaagtagc cgagggaacg ttgtcggtac ggggtaccag catcgagatg gaactcgtgg
     1321 cgacgacgat agggcgcact gcctcggcca aggaggtgac ggcgctcggc cgttccatcc
     1381 gggtgcacgg cgacgagctg acctacacgg tgcacatggg tgctgtcggc cagccactgc
     1441 agcagcattt gacggcgagt ttgcacagga agacggcatg acccccgacg accagcgcga
     1501 agacggcaga atccgtgttc cgaccgatct cgacgcggtc accgacgtcg gcgccgaaga
     1561 ccactccgac atcgacccgg ccgccgtcga gcggatctgg caggcggcac ggcactggta
     1621 tgcc
//
LOCUS       DASYLX010000513         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3644433,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000513 DASYLX010000000
VERSION     DASYLX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3644433"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VGP27_12480"
     CDS             complement(<1..1256)
                     /locus_tag="VGP27_12480"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV7852149.1"
                     /translation="MATDLYSQIVHSAPGNFLAKQLGIPQPETLRRYRAGEPPLAGSL
                     LVGGDGRVVEPLRTALAEDYDVVSNNLGGRWADSFGGLVYDATGITEPAGLKGLYDFF
                     TPLLRNIGPSGRVVVVGTTPEEAGSPHEQIAQRALEGFTRSLAKEMRRGATVALVYLS
                     PDAKPAATGLESTMRFILSGKSAYVDGQVFRVGAADAAPPADWDKPLDGKVAVVTGAA
                     RGIGATIAEVFARDGAKVVCVDVEGAHEPLGVTATKVGGTALTLDVTAADAVDRIAEH
                     VREHHDGRLDILVNNAGITRDKLMANMDEPRWDSVVAVNLLAPLQLTEGLVDNGTLGE
                     GGRVIGLSSMAGIAGNRGQTNYATTKAGMIGLTDALAPKLGDKGITVNAVAPGFIETK
                     MTEAIPVATREVGRRLNSLYQGGKPV"
     gene            1295..2602
                     /locus_tag="VGP27_12485"
     CDS             1295..2602
                     /locus_tag="VGP27_12485"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7852150.1"
                     /translation="MAPMANQSSRRVAVLGGNRIPFARSDGAYASASNQDMFTAALGG
                     LIDRFDLAGERLGAVIGGAVLKHSRDFNLMRECVLGSSLSSYTAAFDIQQACGTGLQA
                     TIAAADGIAAGRYEVAAAGGVDTASDAPIAFGDDLRRVLLGLRRSKSNLDRLKLVGKL
                     PASLGVEIPVNSEPRTGLSMGEHQAITAKQMGIKRTDQDELAAASHNNMAAAYDRGFF
                     DDLVTPFLGVYRDNNLRADSSAEKLAKLKPVFGVKSGDATMTAGNSTPLTDGASVSLL
                     ATEEWASAHGIEPLAYYVDAETAAVDYVNGKDGLLMAPTYAVPRLLARNGLSLQDFDF
                     YEIHEAFASVVLAHLQAWESEEYCKERLGLDKALGSIDRSKLNVNGSSLAAGHPFAAT
                     GGRIVAQLAKQLAEKKKESGKPVRGLISICAAGGQGVAAILEA"
     gene            complement(2708..3823)
                     /locus_tag="VGP27_12490"
     CDS             complement(2708..3823)
                     /locus_tag="VGP27_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV7852151.1"
                     /translation="MTTELARLQNFGPNWFASVMGTGIVATAGITLPVHVPGLRGFSH
                     IVWVVAAALLVVLIVAVTIQWARHPTVARSHVRNPQMTHFYGAAPMALMTVGTGALLT
                     GRDLIGERIAVDLAWMLWSAGTLGGLFTAATIPFLMFTQLNVEPDAAFGGWLMPVVPP
                     MVSAATGALLIPHMAPGTGRTTMLYGCYAMFGLSLIAGLIIITMIWSRLALYGTSGTT
                     RVPTLWIVLGPLGQGITAAGLLGANAALAVSPGLAGAMNAFAILFGVPVWGFAVLWIT
                     LAGQLTVRTLRRGMPFALTWWSLTFPVGTFVTGTTQLANHTGLPAFRVAAVMAYAGLL
                     GTWGLVAAQTARGSVGGSLFAPPPQAGPIKAVKDPPK"
     gene            3854..>4120
                     /locus_tag="VGP27_12495"
     CDS             3854..>4120
                     /locus_tag="VGP27_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7852152.1"
                     /translation="MEVMRISLNLAMAVGTSPIDSIVKALAKLRDEGFRRVWMAQMPT
                     DGDLLTVLAVAMREVDRIEAGSAVLPIQNQHPMLLAQRALTLNSI"
BASE COUNT          676 a         1402 c         1410 g          632 t
ORIGIN      
        1 tcgacgggct taccgccctg atacagcgag ttgagccgac ggcccacctc gcgggtggcc
       61 accgggatcg cctcggtcat cttcgtctcg atgaagccgg gcgcgacggc gttgaccgtg
      121 atgcccttgt cgccgagctt cggcgcgagc gcgtcggtca ggccgatcat gcccgccttg
      181 gtggtcgcgt agttcgtctg gccgcggtta cccgcgatgc ccgccatcga cgaaaggccg
      241 atcacccgac cgccctcgcc aagggtgccg ttgtcgacca ggccctcggt cagctgcagc
      301 ggggcaagga ggttgacggc gacgacggag tcccaccgcg gctcgtccat gttggccatc
      361 agcttgtcgc gggtgatgcc cgcgttgttg accaggatgt cgaggcggcc gtcatggtgt
      421 tcgcgcacgt gctcggcgat gcggtcgacg gcgtcggcgg cggtcacgtc gagcgtgagc
      481 gcggtgccac cgaccttggt cgccgtcact cccagcggtt cgtgagctcc ttcgacgtcg
      541 acgcacacca ccttcgcgcc gtcacgggcg aacacctcgg cgatagtggc gccgataccg
      601 cgtgcggcac cggtcacgac cgcgaccttg ccgtcgagcg gcttgtccca gtcggcgggc
      661 ggggccgcat cggccgcacc gacgcggaac acctggccgt cgacgtaggc cgacttgccg
      721 gacaggatga accgcatcgt cgactcgagt ccggtcgcgg cgggcttcgc gtcgggggag
      781 aggtacacca gcgccaccgt ggcgccgcgc cgcatctctt tggccagcga tcgggtgaag
      841 ccctccagcg cccgctgcgc gatctgctcg tgcgggctgc ccgcctcctc cggcgtcgtg
      901 ccgacgacga ccacgcggcc cgacggcccg atgttgcgca gcagcggcgt gaagaagtcg
      961 tagaggccct tcaggccggc aggctcggtg atgccggtgg cgtcgtacac caggccgccg
     1021 aacgagtcgg cccagcgtcc gccgaggttg ttggacacca cgtcgtagtc ctcggccagc
     1081 gcggtgcgca gcggttccac gacgcggccg tcaccaccga cgagcaggga acccgccagc
     1141 ggcggttctc ccgctcggta gcggcgcagc gtctcgggct gcgggatgcc gagctgcttg
     1201 gcgaggaagt ttcccggcgc tgaatgaacg atttgcgagt acaggtcggt agccacgaca
     1261 ccgaacttac tcttgagtaa taagggtgcg taatatggcc cccatggcca accagtcgag
     1321 tcgccgcgtt gccgttcttg gcggcaaccg gatcccgttc gcacgctccg acggcgcgta
     1381 cgccagcgcc tccaaccagg acatgttcac cgccgcgctc ggcgggctga tcgaccgctt
     1441 cgatctggcg ggcgagaggc tcggcgccgt gatcggcggc gcggtgctca agcacagccg
     1501 cgacttcaac ctgatgcgcg aatgcgtgct gggcagctcg ctgtcgtcct acaccgccgc
     1561 gttcgacatc cagcaggcct gcggcaccgg cctgcaggcg accatcgccg cggccgacgg
     1621 aatcgccgca gggcggtacg aggtggcggc ggccggcggt gttgataccg cctcggacgc
     1681 accgatcgcg ttcggtgacg acctgcgtcg ggtgctgctc gggctgcgcc ggtcgaagtc
     1741 caacctggac cgcctcaagc tggtcggcaa gctgccggcg tcgctgggtg tggagatccc
     1801 ggtcaacagt gaaccgcgca ccggtctgtc gatgggcgaa caccaggcca tcaccgccaa
     1861 gcagatgggc atcaagcgca ccgatcagga cgaactggcc gccgccagcc acaacaacat
     1921 ggcggccgcc tacgaccgcg gcttcttcga cgacctggtc accccgttcc tcggtgtcta
     1981 ccgggacaac aacctgcggg ccgactcgtc ggcggagaag ctggccaaac tcaagccggt
     2041 gttcggcgtg aagtccggcg acgcgacgat gaccgcgggc aactccactc cgctgaccga
     2101 cggcgcgtcg gtgtcgctgc tggccaccga ggagtgggcg tcggcacacg gcatcgaacc
     2161 gctggcgtac tacgtcgacg ccgagacggc ggcggtggat tacgtcaacg gcaaggatgg
     2221 cctgctgatg gcgccgacct acgcggtgcc gagactgctg gcgcgaaacg gcttgagcct
     2281 gcaggacttt gacttttacg agatccacga ggcgttcgca tcggtggtgc tggcgcacct
     2341 gcaggcgtgg gagtccgagg agtactgcaa ggagcggctc ggcctggaca aggcactggg
     2401 ctcgatcgac cgctccaagc tgaacgtgaa cggctcgtcg ctggcggccg gccatccgtt
     2461 cgccgcgacc ggcggccgga tcgtcgcgca actggccaag cagctcgccg agaagaagaa
     2521 ggaaagcggc aagcccgtgc gcggcctgat ctccatctgc gctgccggtg ggcagggcgt
     2581 cgccgccatt ctcgaggcct gacggagaaa cttcgacggg ggtgtaacgg cccggcgggc
     2641 acccccgata cacgggatag ctccgtgttg tcgtctgacc ccccgacccg acggcaacac
     2701 ggggcgacta cttcggcggg tccttgaccg ccttgatcgg ccccgcctgc ggcggcggcg
     2761 cgaacagact tccgccgacg ctgccccgag cggtctgcgc cgcgaccagc ccccacgtac
     2821 ccagcagccc ggcataggcc atgacggcgg cgacgcggaa cgcgggcagg ccggtgtggt
     2881 tggccagttg cgtggtgccg gtgacgaatg tcccgacggg gaacgtcaaa ctccaccacg
     2941 tcagtgcgaa cggcatgccg cgacgcagag tgcgcacggt gagctggccg gccagcgtga
     3001 tccacagcac cgcgaatccc cataccggga cgccgaagag gattgcgaag gcgttcatcg
     3061 cgcctgccag tcctggcgag acggccaacg cggcgttcgc accgagcagg cccgccgccg
     3121 tgatgccctg gccgagcgga ccgaggacga tccacagggt cggcacccgt gtcgtcccag
     3181 acgtcccgta gagcgcgagc ctgctccaga tcatcgtgat gatgatgagc ccagcgatca
     3241 gcgaaaggcc gaacatcgca tagcagccgt acagcatcgt ggtgcgcccc gtgcccggcg
     3301 ccatgtgcgg tatcagcagg gcgccggtcg ccgccgaaac catcggtggc acgaccggca
     3361 tcagccagcc gccgaacgcc gcatcgggtt cgacgttgag ctgggtgaac atcaggaacg
     3421 ggatcgtggc cgcggtgaac aggccgccaa gggtgcctgc gctccacaac atccaggcga
     3481 ggtcgacggc gatccgctcg ccgatcaggt ccctgcctgt cagcagggca ccggtgccca
     3541 ccgtcatcag tgccatcggc gcggcgccgt agaagtgcgt catctgcggg ttgcgcacat
     3601 ggctgcgcgc caccgtggga tggcgcgccc actgaattgt caccgcgacg atgagcacca
     3661 ccagcagcgc cgcggcgacc acccacacga tgtgtgagaa cccgcgcagc ccgggcacgt
     3721 gcactggaag cgtgatgccc gcggtggcga cgatgccggt gcccatcacc gacgcgaacc
     3781 aattcggccc gaagttctgc agccgcgcca attccgttgt catgggtgca catcgtttca
     3841 cgcgcgtgct tcgatggagg tcatgcggat cagcctcaat ctcgccatgg ccgtcggtac
     3901 atcgccgatc gattcgattg tgaaggcgct tgcgaagttg cgtgacgagg gtttccgcag
     3961 agtgtggatg gcgcagatgc cgaccgacgg cgacctgttg accgtgctgg cggtggcgat
     4021 gcgcgaagtc gaccgcatcg aggctggctc cgctgtgcta ccgatccaga atcagcaccc
     4081 gatgttgctc gcccagcgcg ctctgacgct caactcgatc
//
LOCUS       DASYLX010000514         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3755492,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000514 DASYLX010000000
VERSION     DASYLX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755492"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGP27_12500"
     CDS             complement(<1..893)
                     /locus_tag="VGP27_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7852153.1"
                     /translation="MTALRKILVIACVIALATFGAAACGKKSEGGGGGGGGGEINVAL
                     TSFPDYVDPQLSYTVEGWCVLWNVYTPLLTYKHAKGKDGTQLVPALAEAMPEISADGK
                     TYKLKLRSNMKYSDGTPIKASDFAYGIQRLFKTDSGGSVFYDNIVGAAEYADGKAQTI
                     TGIKTDDNTGDITITLKEPNGTFENVLGLMFAAPVPPSTPLDKDSTNKPAAASGPFMI
                     SSVNAPRTLTMERNPNFKTVKDAGASDVPDASVDKIVVNENKNNSAQVTDVEQNKVDF
                     MLDPPVADRLGEVKAKFSDRFR"
     gene            1173..1451
                     /locus_tag="VGP27_12505"
     CDS             1173..1451
                     /locus_tag="VGP27_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852154.1"
                     /translation="MLCTARRLAAAGAAAVAPLVFATIVTPGIGNAVECGWGTVYDAP
                     SNSCVAAPAPPPPAPPPPAWNGDITPYFGVGVCVPIPVPFAPSICGGI"
     gene            1569..1865
                     /locus_tag="VGP27_12510"
     CDS             1569..1865
                     /locus_tag="VGP27_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852155.1"
                     /translation="MRTQSRLPRHLAAVAGLSALAAMGMITAGCAKEQQKAPETSTTT
                     TTTTTTSPAAPVSPTEKAPRIDPQAPNQFTPPVLAPPPSQAQPGEHGRHVTNGS"
     gene            1879..3468
                     /locus_tag="VGP27_12515"
     CDS             1879..3468
                     /locus_tag="VGP27_12515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV7852156.1"
                     /translation="MRRRISALVAASSLVVGCGLSAGCTPTSSALIGGPFASLLAGSA
                     DLGPSRSGDAQLTVALPYAARPDVLMSWASARGLRVRWRPGDQWAIVEGAAQDVAHAF
                     GVPVNDYRGRKGQVFYASPQQPSVPVALDDDVTGLGRIIGYTPHRTKRPDNIPLDVPK
                     GGLRPGGLLDAYNADPLVKEGHTGKGETIVFFEFDGFDQSDLDKYADTSGLPRFTPEV
                     IGGVADEAHGETTMDLQVAHAIAPDAHLVYINAGPTLEGDGAYEKIGRMFEDADRRFP
                     GAVWSLSIGWSCDSFVNATDLAPVESALVTAQSHGTSAFDATGDNAGLECKGADEWSS
                     PPAPDDVGLDAVSSLPTMTAVGATTLAVGPRGQWLSEYAWFDSPLSQGTSGGVSGLFA
                     RPQWQEKLTTDRDTTRRRLSPDVAAVGDPLTGVRFVFGHNEYVGAGTSQAAPIWAALT
                     VLMNQYLKANGGRPLGNLNPLLYRVAAGSDLPGFRDISLGGNAVDLSRPGYDVVTGLG
                     SPNTDNLARNLLKIQSQVAPE"
BASE COUNT          576 a         1142 c         1163 g          666 t
ORIGIN      
        1 cggaaccggt cgctgaactt cgccttcacc tccccgagcc ggtccgcgac gggcggatcg
       61 agcatgaagt cgaccttgtt ctgctcgacg tcggtcacct gggcgctgtt gttcttgttc
      121 tcgttgacga cgatcttgtc gacgctggcg tcgggcacgt cggatgcgcc ggcgtccttg
      181 actgtcttga agtttggatt gcgctccatc gtcagcgtgc gcggggcgtt gacgctactg
      241 atcatgaacg ggccgctggc tgccgcaggc ttgttcgtcg aatccttgtc cagcggggtg
      301 ctcggcggca cgggtgcggc gaacatcagt ccgagcacgt tctcgaaggt gccgttgggc
      361 tccttgagcg tgatggtgat gtcaccggtg ttgtcgtcgg ttttgatgcc ggtgatggtc
      421 tgggccttgc cgtcggcgta ctcggcggcg cccacgatgt tgtcgtagaa caccgagccg
      481 ccggagtcgg tcttgaagag ccgctggatc ccgtaggcga aatcggacgc cttgatgggc
      541 gtgccgtccg aatacttcat gtttgagcgc agtttcagct tgtaggtctt gccgtccgcc
      601 gagatctcgg gcatggcctc agcgagagca ggcaccagct gcgtgccgtc tttgcccttg
      661 gcgtgtttat aggtaagcaa gggcgtgtag acgttccaga ggacgcacca cccctcgacg
      721 gtgtaggaca gctgcgggtc gacatagtcg gggaacgagg tcaacgcgac gttgatctcg
      781 ccgccgcccc cgccgccccc gccgccttcg ctcttcttgc cgcacgccgc ggccccgaac
      841 gtagccaagg cgatgacaca ggcgatgacg aggatcttgc gcagtgctgt catggtgctc
      901 cctctccgca ggcgatccag gcaaccccat acacctacgc cacttctgct ggacttggtc
      961 gggctttcgg ggaatctccg ttcatgcacc ttctgccaca tgaagtagca ccgcaactcg
     1021 acggtggcgg gcgcgcgagc ccgcaccctt cgaaggtttc ggcggcagtc ctcatgctgg
     1081 gtcttcgtgc ggttcattcg ctttccgcga atcgcttaac cggcctaaga tcgggctgtt
     1141 gagggtcggg gacagaccga gggaggcctt cgatgctgtg cacagcccgt cgtctggcag
     1201 ccgccggcgc ggctgcggtt gcgccgctcg tgttcgccac gatcgtcaca ccagggatcg
     1261 gaaacgcagt cgaatgcggc tggggaaccg tgtacgacgc gccgtcgaac agctgcgtgg
     1321 ctgcgcccgc tccgccgccg ccggcaccgc cgccacctgc gtggaacggg gatatcacgc
     1381 cgtacttcgg ggtaggagtt tgcgtgccga tcccggtacc gtttgctccg tcgatctgcg
     1441 gaggcattta aatgttcttc ccggccgtta aattttgcta tgggactggc gcggcttcgc
     1501 tgtatgtgtc accatggtcg gtaattcctg ccgattgtcg gtgcaggtac acagataagg
     1561 acaagtgaat gaggacacaa agtcgtttgc ctcgacatct cgcggcggtt gccgggttga
     1621 gcgctctcgc ggccatgggc atgatcaccg cggggtgcgc aaaggaacag caaaaggctc
     1681 cggaaacctc gacaacgacc accaccacga cgacgacgtc gcctgccgct ccggtctcgc
     1741 ccaccgagaa ggctccgcgg atcgacccac aggcgccgaa ccagttcacg cctcccgttc
     1801 ttgcgccgcc gccctcgcag gctcagcccg gcgaacatgg tcggcacgta acgaacggct
     1861 cgtaggccga ttgccgaaat gcggcgtcga atctcggcgc tcgtcgcggc gtcgtcactg
     1921 gttgtcgggt gcggcctgag cgcaggctgc acgccgacca gttcggctct catcggcggt
     1981 cctttcgcct cgctgcttgc cggctcggca gacctcggac cgtcgcgctc cggcgacgcg
     2041 cagctgaccg tcgcactgcc ctacgcggct cgccccgacg tgctgatgtc gtgggcgagt
     2101 gcgcggggat tgcgggtgcg gtggcgtccc ggcgaccaat gggcgatcgt cgagggcgcc
     2161 gcccaagatg tggctcacgc gttcggcgtg ccggtcaacg attaccgcgg ccgaaaaggc
     2221 caggtgttct acgcgtcacc gcagcagccg tctgtgcctg tggcgcttga tgatgacgtc
     2281 accgggctag gtcggatcat cggctacacg ccacatcgca ccaaacgacc ggacaacatt
     2341 ccgcttgatg tgccgaaggg cgggttgcgt ccgggcgggt tgttggacgc ctacaacgcc
     2401 gacccgctgg tgaaggaggg ccacaccggc aagggcgaaa ccatcgtgtt cttcgagttc
     2461 gacggcttcg atcagagcga cctcgacaag tacgccgaca cctcaggcct gccgcggttc
     2521 acacccgagg tgatcggcgg ggtggccgac gaagcccacg gcgagacgac gatggatctc
     2581 caagtggcac acgccattgc accggatgcg catctggtct acatcaacgc cggaccaacc
     2641 ctcgagggcg acggggcgta cgagaagatc ggcaggatgt tcgaggacgc cgatcggcgg
     2701 ttccccggcg cggtgtggag cctgtcgatc ggatggtcct gcgactcctt tgtcaacgcc
     2761 acagacttgg ctccggtcga gtccgcattg gtcacggcgc agtcacacgg cacctcggcg
     2821 ttcgacgcga ccggggacaa cgcgggcctc gagtgcaaag gcgccgacga gtggtcgtcc
     2881 ccgccggctc cggatgacgt tgggctggac gcggtttcat cgttgcccac catgaccgca
     2941 gtcggtgcca ccactctcgc ggtcgggcca aggggtcaat ggctctcgga atacgcgtgg
     3001 tttgattcgc cgctgtccca ggggaccagc ggcggtgtct cagggctgtt cgcgcggccg
     3061 caatggcagg agaagttgac caccgatcgc gacacgacgc ggcgccgact gtcaccagac
     3121 gtcgccgcgg tcggcgaccc gctcaccggg gtgcggttcg tcttcgggca caacgagtac
     3181 gtcggcgcag gcacgtccca ggccgcaccc atctgggccg cgctgacggt gttgatgaac
     3241 caatatctga aggccaacgg cggccggccg ctgggaaacc tcaacccact tctgtatcgg
     3301 gttgcggccg gatccgacct accaggattt cgggatatct ccctgggcgg caacgccgta
     3361 gacctctctc ggcccggcta cgatgtcgtg accggactcg gcagtcccaa cactgacaac
     3421 ctcgccagga accttctcaa gatacagtcg caggtagcgc ccgaatgaca caaccgcccg
     3481 ccggcgatga cggcgtggcc accatctcgt gcggttcctg cggtaccgag gtgtcggccg
     3541 cggcgtt
//
LOCUS       DASYLX010000515         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1889162,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000515 DASYLX010000000
VERSION     DASYLX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889162"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VGP27_12520"
     CDS             complement(<1..95)
                     /locus_tag="VGP27_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7852157.1"
                     /translation="MSEGRLFTSESVTEGHPDKICDAISDSVLDAL"
     gene            complement(228..1469)
                     /gene="coaBC"
                     /locus_tag="VGP27_12525"
     CDS             complement(228..1469)
                     /gene="coaBC"
                     /locus_tag="VGP27_12525"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV7852158.1"
                     /translation="MDRKRIIVGVSGGIAAYKACTVVRQLTEAGHFVRVIPTESALRF
                     VGAATFEALSGHPVHTGVWDDVHEVPHVRIGQEADLVLVAPATADLLARAVAGRADDL
                     LTATLLTARCPVLYAPAMHTEMWFHPATIDNVATLRRRGAVVVEPASGRLTGADSGAG
                     RLPEAEEITTLAHLLLERADSLPYDLAGVKVLVSAGGTREPLDPVRFIGNRSSGKQGY
                     AMARVAAQRGAEVTLIAGNTAGLIDPAGVDVVHIGSAAQLKDAVSKHAPDAHVLVMAA
                     AVADFRPSHFETNKIKKGASEPTALELTRTDDVLVGAVRARTDGQLPNMRAIVGFAAE
                     TGDANGDVLFHARAKLARKGCDLLVVNAVGENRAFEVDSNDGWLLASDGTEVALEHGS
                     KTLMASRIVDAIVAFLHNGGG"
     gene            complement(1484..1771)
                     /gene="rpoZ"
                     /locus_tag="VGP27_12530"
     CDS             complement(1484..1771)
                     /gene="rpoZ"
                     /locus_tag="VGP27_12530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV7852159.1"
                     /translation="MAAVKDLHTAHSGYDTPLGITNPPIDDLLDRASSKYALVIYAAK
                     RARQINDYYNQLGDGILEYVGPLVEPGLQEKPLSIAMREIHEDLLEHTEGE"
     gene            complement(1873..>2130)
                     /locus_tag="VGP27_12535"
     CDS             complement(1873..>2130)
                     /locus_tag="VGP27_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV7852160.1"
                     /translation="VDLAGARAVKHAIPEAVTVFLAPPSWEVLQGRLTGRGTETREVM
                     DRRLATARAELAAKEHFDRIVVNSQLESACAELVSLLVDPR"
BASE COUNT          321 a          692 c          739 g          378 t
ORIGIN      
        1 agcgcgtcga gcaccgagtc gctgatcgcg tcgcagatct tgtcggggtg cccttcggtc
       61 accgactcac tggtgaacag ccgaccttca ctcacggcgt cattcctctc gctgcagctt
      121 tcccataaga aacttagttg atcaaattat atgtgcgacc ctgttgcacc caagcgcgcg
      181 cccccacggc acgcaaccgt tacgcgcaag ccgatgtgtg cgtctgctca cccgccgccg
      241 ttatgcagga acgcgacaat cgcgtccacg atacggctgg ccatcagagt tttcgaaccg
      301 tgctccagcg ccacctcggt gccgtccgat gcgagcaacc atccgtcgtt gctgtctacc
      361 tcgaaagcgc gattctcgcc gacggcgttg acgacgagca agtcgcagcc tttgcgcgcc
      421 agcttggcgc gggcgtgaaa cagtacgtcg ccgttcgcgt cgccggtctc ggcggcgaat
      481 ccgacgatcg cgcgcatgtt gggcagctgg ccgtcggttc gcgcccgcac cgcgccgacc
      541 agcacgtcgt ctgtgcgggt gagttcgagc gcggtgggct cggacgcgcc cttcttgatc
      601 ttgttggtct cgaagtgcga gggccggaag tcggcgacgg cggcggccat cacgagaacg
      661 tgcgcgtcgg gtgcgtgctt ggacaccgcg tctttgagtt gagccgccga cccgatgtgg
      721 accacgtcga cacccgccgg atcgatgagg cctgcggtgt tgcctgcgat cagcgtgact
      781 tcggcgccgc gctgcgccgc gacgcgggcc atcgcgtagc cctgctttcc cgaggaccgg
      841 ttgccgatga aacgcacggg gtcgagcggt tcgcgggtcc cgccggccga gacgagcacc
      901 ttgacgcctg cgaggtcgta gggcagcgag tcggcgcgct cgagaagcag gtgggccagc
      961 gtggtgatct cttcggcctc gggcagccga ccggcgccgc tgtcggcgcc ggtgagccgg
     1021 ccggacgcgg gctcgaccac gacggctccg cggcggcgca gcgtcgccac gttatcgatg
     1081 gtggccgggt gaaaccacat ctcggtgtgc atcgcgggtg cgtacaacac cggacatctg
     1141 gcggtcagca gagtggcggt caacagatcg tcggcccggc ccgcaacggc tcgggcgagc
     1201 aggtcggcgg tggcaggcgc gacgaggacg aggtcggctt cctgcccgat ccggacgtgc
     1261 gggacctcgt ggacgtcatc ccagacacct gtgtgcacgg gatgaccgga gagcgcctcg
     1321 aaggttgccg cgccgacgaa ccgcagcgcg gactcggtcg gaatgacgcg gacgaagtgg
     1381 ccggcctcgg tgagctggcg gaccaccgtg cacgccttgt aggcggcgat gccgcctgat
     1441 acgccgacga tgatccgctt gcggtccacc ttcgaacgcc tggctactcg ccctcggtgt
     1501 gctcgagcag gtcctcgtgg atctcgcgca tcgcgatcga cagcggcttc tcctgcaggc
     1561 cgggctcgac cagcgggccg acatactcga ggatgccgtc gccgagctgg ttgtagtagt
     1621 cgttgatctg ccgtgcgcgc ttggcggcgt agatcaccag cgcgtacttg ctcgacgcgc
     1681 gatcgagcag gtcatcgatg ggcgggttgg tgatgcccag cggtgtgtcg tagccgctgt
     1741 gggccgtatg gaggtccttg acggcggcca acggcgcgtc ggcgtgcggg gtgctcacta
     1801 aaaaattctc ctggcgtttg gtgaaatgct cacgtggctt gcgggccgca tcgcgccgtt
     1861 gccggcgcct ggttatctgg ggtccaccag caaggatacc aattcggcgc aggcagactc
     1921 caattgactg ttcacgacga tgcggtcgaa gtgctccttg gcggccagtt cggcgcgtgc
     1981 cgtggccagc ctgcggtcca tgacttcgcg ggtttcggtg ccccggccgg tgagtcggcc
     2041 ctgcagcacc tcccaactgg gcggcgcgag gaacaccgtg acggcctcgg gtatcgcgtg
     2101 cttgacggcc cgcgcaccgg cgagatcgac
//
LOCUS       DASYLX010000516         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1011681,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000516 DASYLX010000000
VERSION     DASYLX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011681"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VGP27_12540"
     CDS             complement(<1..767)
                     /locus_tag="VGP27_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852161.1"
                     /translation="MSILVGFAPWIVYWVLVGNVPFETAVLVALAVAVASFVIGRVKG
                     SPGRTLEIGAIGTFVVLAVLTFTASQSFMEEWMQPLSNAGIFLVALASMLIGRPFVRE
                     FAVVGQPEEVINSETFNKITTLLTWIWVGAFAGMTVSSAIPPIVYGHATILDTRTPLS
                     FTCYWVIPFALLGLAALASRLLPERMGPSDDEERKTSFVMFAEAEIDQLMYAATEAVN
                     REVGSGKEAYDIRIGSKGIPLSGDETRESWPATYKLRT"
     gene            complement(862..1200)
                     /locus_tag="VGP27_12545"
     CDS             complement(862..1200)
                     /locus_tag="VGP27_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEV7852162.1"
                     /translation="MLVHICASDDWQLAKDLGVHRPESLETVGFIHLSTPEQLHLPAN
                     RLYAGRTDLVLLHIDPDRLTDPVRWEPGVPADPESMLFPHLYGPLPADAVTSVTPYRP
                     GPDGSFLPLT"
BASE COUNT          303 a          489 c          471 g          243 t
ORIGIN      
        1 gtgcggagtt tgtatgtcgc aggccatgat tcgcgggtct cgtcgccgga cagcgggatg
       61 cccttgctcc cgatcctgat gtcgtaggct tccttgccgg aaccgacctc tcggttgacc
      121 gcttctgtgg cggcgtacat caactggtcg atctcggctt cggcgaacat gacgaacgac
      181 gtcttacgct cctcgtcgtc gctcggtccc atcctctccg gcagcagccg cgacgccagc
      241 gcggcgaggc ccaacagcgc gaacgggata acccagtagc aggtgaacga cagcggcgtc
      301 ctggtgtcca ggatcgtggc atggccgtac acgatggggg ggatcgccga tgacaccgtc
      361 atgccggcga acgcaccgac ccagatccac gtcagcagcg tggtgatctt gttgaacgtc
      421 tcgctgttga tcacttcttc cggctggccc accacggcaa actcgcgcac gaacggcctg
      481 ccgatcaaca tgctggccaa cgcgaccaag aagatgcccg cgttgctcag cggctgcatc
      541 cactcctcca tgaacgactg gctcgccgtg aacgtcagca ccgcaagcac gacaaaggtt
      601 ccaatggcac cgatttccag cgtgcgcccc ggcgacccct tcacccggcc gatcacgaac
      661 gacgccaccg cgacggcgag tgcgaccagc accgcggtct cgaacgggac gttgcccacc
      721 agcacccaat agacgatcca cggcgcgaag cccaccagaa tgctcacggt gggtcagtgt
      781 atgtagtcgg ctccgcgagg acgacagtca gcaccgaatc tgtaacggcg agtcagtgcg
      841 gcaacgccga gaggtcgacg cctatgtcag cggcaggaac gagccgtcgg gccccggccg
      901 ataaggcgtg acgctcgtca cggcgtcggc gggcagcggg ccgtacagat gcgggaagag
      961 catcgactcc ggatctgccg gcacgcccgg ttcccaccgg acgggatcgg tcaaccggtc
     1021 gggatcgatg tgcagcaaca ccaggtccgt gcggcctgcg tagagccggt tggccggcag
     1081 atgtagctgc tcaggcgtgg agagatgaat gaagccgaca gtttcgaggg attccggccg
     1141 gtgcactccg agatccttgg cgagctgcca atcgtccgag gcacagatgt gcacgagcat
     1201 tcctccaccc tgcccgctcg gcaacttcgc tgagcgtgaa aaacgacaca cccgtgcacg
     1261 gcctgggaac aacgccgaaa ccgtaagcgt ctgacagagt ggagatgcgt gaaaccgggt
     1321 attaaccggt gtcacacgag cagagacgga ggagccatga acgcaactct gaccagtccc
     1381 gaactgaccc gggctgatcg ctgcgatcgc tgcggcgccg ctgctcgcgt acgagcaacg
     1441 ttgccgtcgg gagccgagct gttgttttgt cagcaccacg ccaatgagca cgaagccaag
     1501 ctggtc
//
LOCUS       DASYLX010000517         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1450388,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000517 DASYLX010000000
VERSION     DASYLX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450388"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="trpB"
                     /locus_tag="VGP27_12550"
     CDS             complement(<1..1055)
                     /gene="trpB"
                     /locus_tag="VGP27_12550"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV7852163.1"
                     /translation="MADLAGPELPRSSAAVAEPTAHDPDARGHFGVYGGRFVPEALMA
                     VIEEVTASYEKARGDQAFLDELDRLQRHYSGRPSPLYEAARLSGHAGGARIFLKREDL
                     NHTGSHKINNVLGQALLARQMGKKRVIAETGAGQHGVATATACALLGLDCVIYMGAVD
                     TARQALNVARMRLLGAEVVSVESGSKTLKDAINEAFRDWVTNADDTYYCFGTAAGPHP
                     FPVMVRDFQRVIGLEARVQIQDQAGRLPDVVTACVGGGSNAIGIFHAFIDDPGVKLVG
                     FEAAGDGVETGRHAATFTGGSPGAFQGSFSYLLQDDDGQTIESHSISAGLDYPGVGPE
                     HAFLKDIGRAEYRPITDT"
     gene            complement(1059..1877)
                     /gene="trpC"
                     /locus_tag="VGP27_12555"
     CDS             complement(1059..1877)
                     /gene="trpC"
                     /locus_tag="VGP27_12555"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV7852164.1"
                     /translation="MSSATVLDSIIEGVRADVAAREAVVSLDEVKAAAKAAAAPLNVM
                     AALREPGIGVIAEVKRASPSRGALATISDPGKLAGAYEDGGARIISVLTEERRFNGSL
                     DDLDVVRAAVSIPVLRKDFIVRPYQIHEARAHGADMLLLIVAALEQSALVSMLDRTES
                     LGMTALVEVHTEEEADRALQAGAKVIGVNARDLKTLNVDRDCFARIAPGLPTNTIRIA
                     ESGVRGPADLLAYAGAGADAVLVGEGLVTSGDPRSAVADLVTAGTHPSCPKPSR"
     gene            complement(1962..2558)
                     /locus_tag="VGP27_12560"
     CDS             complement(1962..2558)
                     /locus_tag="VGP27_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02234 family membrane protein"
                     /protein_id="HEV7852165.1"
                     /translation="MRHIGQLVLLVAAGALWLASRMTWVVVSSFDGLGQPETATLNGA
                     AWSTALVPLALILLAAALKSMVGPRWQLRLLALVVGALSTVMAYLAISLWVIPDVAVR
                     AAGLAEVPLADLVGTQRHYWGAIVALIAAVLTLAGAVLLLRAPVKNESKDEKYEAPAR
                     RRETVRRRAADGDDMSERMMWDALDDGRDPTKPDTEGR"
     gene            complement(2555..>2804)
                     /locus_tag="VGP27_12565"
     CDS             complement(2555..>2804)
                     /locus_tag="VGP27_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEV7852166.1"
                     /translation="IEEVEKTRRGLYGGVLGYLDFAGNADFAIAIRTALMRERTAYVQ
                     AGGGVVADSNGPYEYNEAANKAKAVLNAIAAAETLTEP"
BASE COUNT          469 a          994 c          872 g          469 t
ORIGIN      
        1 gtgtcggtga tcggccggta ctcggcacgc ccgatgtcct tcaggaaagc atgttctggg
       61 ccgacgccgg gatagtccag acccgccgag atcgaatgcg attcgatcgt ctgcccgtcg
      121 tcgtcctgca acagatagga gaaggagccc tgaaacgcgc cgggcgaccc accggtgaac
      181 gtcgccgcgt gacggcccgt ttcgacaccg tcgcccgcag cctcgaaacc gaccagcttc
      241 acacccggat catcgatgaa cgcgtggaag ataccgatgg cgttggaccc gccgccgacg
      301 catgcggtca ccacgtcggg taaccggccg gcctggtcct ggatctggac gcgcgcctcg
      361 agaccaatca cccgttgaaa gtctcggacc atcaccggga atggatgcgg acccgccgcg
      421 gtgccgaagc agtaataggt gtcgtctgcg ttggtcaccc agtcgcggaa cgcctcgttg
      481 atcgcatcct tgagtgtctt cgagccggac tcgacagaga ctacttcagc tcccaacagc
      541 cgcatccgcg caacgttcaa cgcctggcgg gcggtatcga ctgcgcccat gtagatcacg
      601 cagtccaggc ccaacagcgc acacgcggtc gcggtggcaa cgccgtgctg accggcgccc
      661 gtctcggcga tgacacgctt cttgcccatc tggcgcgcga gcaaggcttg acccagcacg
      721 ttgttgatct tgtgagaccc ggtgtgattc aggtcttctc gcttgaggaa gattcgcgca
      781 ccacccgcat gcccgctgag cctcgcggct tcgtaaagcg gtgatggccg accgctgtaa
      841 tgccgctgca gtcggtccaa ttcatcgagg aatgcctggt cgccgcgcgc cttctcgtaa
      901 gaggcggtga cctcctcgat caccgccatc agcgcctcgg gaacgaatcg tcctccgtag
      961 acaccgaaat gacccctggc atcgggatcg tgagctgtgg gttccgcgac cgccgcgcta
     1021 gaacgcggca actcggggcc ggcgaggtcg gccacttctc agcgcgaggg tttcgggcag
     1081 gacgggtgcg tgcctgcggt gaccagatcg gcgacggcgc tgcgtggatc accgctggtg
     1141 accagaccct cgccgacgag aacggcgtca gcacccgcac cggcgtacgc cagaagatcc
     1201 gccggaccgc gcacgcccga ctctgcgatg cggatggtgt tggtcggcag gcccggcgcg
     1261 atgcgcgcga agcaatcccg gtcgacgttg agcgtcttca gatcgcgcgc gttgacgccg
     1321 atcaccttcg cgcctgcctg aagcgcccga tcggcctcct cttcggtgtg cacctcgacc
     1381 aacgcggtca ttcccaacga ctcggtgcga tcgagcatcg acaccagcgc ggactgttcg
     1441 agcgctgcaa cgatgagcag cagcatgtcg gcaccgtgcg cgcgggcctc gtggatctgg
     1501 tacggccgga cgatgaaatc tttgcgcaac accgggattg acaccgcggc gcgcacgacg
     1561 tccaggtcgt cgagcgaccc gttgaacctg cgctcttcgg tcagcacgct gatgatgcgc
     1621 gcaccgccgt cctcgtatgc gccggcgagc ttccccggat cggagatcgt cgcaagagct
     1681 ccccgagacg ggctggcgcg cttcacttcg gcgatgacgc cgatgccggg ttcgcgcagc
     1741 gccgccatca cgttcagcgg cgcggccgcg gccttcgcgg ccgccttgac ctcgtcaaga
     1801 ctgacgacgg cctcgcgcgc ggcgacgtct gcgcgaactc cctcaatgat ggagtcgagc
     1861 acagtcgccg aactcataat ccgtcgactc cctccccgtt cgcgccgttt ccctgacgga
     1921 tgatcttcat cgaagggtaa ccgccccgag cacaccatct gtcaccgccc ctcggtgtcc
     1981 ggtttggtgg gatcacgccc gtcgtcgagt gcgtcccaca tcatccgctc cgacatgtcg
     2041 tcgccgtccg cggcccgtcg ccgcaccgtc tcgcgtcgcc gcgccggagc ctcgtatttc
     2101 tcgtctttcg actcgttctt cacgggtgcc cgcagaagca gcaccgcgcc cgcgagggtg
     2161 agcacggcgg caatcaaggc gacgatggca ccccagtaat gccgctgcgt gcccaccaga
     2221 tccgcaagcg gcacctccgc gaggccggcg gcgcgcacgg ccacatcggg aatcacccac
     2281 agactgatcg ccaggtaggc cattacggtg ctcagcgcgc cgaccaccag cgccagaagc
     2341 cgcagctgcc atcgcggccc caccatcgac ttgagcgccg ctgccagcag gatcagggcc
     2401 agcggcacca gcgccgtcga ccacgctgcg ccgttcagcg tcgcggtctc gggttgcccc
     2461 aggccgtcga acgagctcac cactacccac gtcatccgcg acgccagcca cagcgcgccc
     2521 gccgccacca acagcaccag ctgcccgatg tgtctcatgg ttcggtcagc gtctcggcag
     2581 cagcgatggc gttcagcacg gctttggcct tgttcgcagc ctcgttgtac tcgtaggggc
     2641 cgttggagtc ggccacgacg cctccgccgg cctgcacata tgctgtccgc tcgcgcatca
     2701 gggcggtgcg gatggcgatc gcgaagtctg cgttgcctgc gaagtccagg tagccgagca
     2761 caccgccgta gaggccgcgg cgggtcttct cgacctcctc gatg
//
LOCUS       DASYLX010000518         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3755809,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000518 DASYLX010000000
VERSION     DASYLX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755809"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VGP27_12570"
     CDS             complement(<1..653)
                     /locus_tag="VGP27_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HEV7852167.1"
                     /translation="MSNPSGPGQGHQPAVESTGEPADDASTTEDSARTEAAPVVSAEA
                     GPIVEEHPTEVMAVEDTGALEPDTQPEEPAERRYTAPSGMDSSATKFIERPPDPATEV
                     FSTPTQPISGKSVAPQLIPGRGDAPNPPGRRRSWGWVVALILVIAALAAVAILGTLLL
                     TRGSDSKESQQDMVRTTIQNFDVAVQNGDLATLRSITCGATRDSYVNYDDRAWADTHA
                     "
     gene            744..1160
                     /locus_tag="VGP27_12575"
     CDS             744..1160
                     /locus_tag="VGP27_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HEV7852168.1"
                     /translation="MTRMGDLLGPDPTLLPGDPAAEAELDADEKPAIVAAAHPTASVA
                     WAVLAERALADDKAITAYAYARTGYHRGLDQLRRNGWKGFGPVPYSHAPNRGFLRCVA
                     ALAKAADAIGESDEYSRCLDLLDDCDPSARAELGVG"
     gene            complement(1163..2056)
                     /locus_tag="VGP27_12580"
     CDS             complement(1163..2056)
                     /locus_tag="VGP27_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7852169.1"
                     /translation="MGAGHNHGSGDTRVSRMIIAAAVLSVFFVIELTTALLINSIALL
                     ADAGHMLTDLVAMFMGLTAVLLARHGSASAKRTYGWHRAEVFTAVANAVLLLFVAVFI
                     LYEAFERLGDAPDVPGVPMIVVALAGLIANAIVMLLLRSQSGQSLAVKGAYMEVVADT
                     VGSLGVLIAGIVTVTTRWPYADVVVAVFVALWVLPRAIALARAALRILSENSPRHIDV
                     AELRIALCAVAGVTEVHDLHVWTLVPGKDMVTAHLTSTADSARVLDEAKAVLIARGLD
                     HATVQVEPPDAAGDCHCETSW"
     gene            complement(2102..2881)
                     /locus_tag="VGP27_12585"
     CDS             complement(2102..2881)
                     /locus_tag="VGP27_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV7852170.1"
                     /translation="MTIRPAHQSVRPSPMFLIVVAVTAAGAVLAWLAQSSRTPLAYMG
                     VFVFVIAGWIVSLCFHEFGHAFTAWRYGDHDIAVRGYLTLNPFKYANPMLSLILPVLI
                     TLLGGIGLPGGAVWVRTSFMTDRQKTIVSLAGPFMNAVLAVLLLVATRLFYNPEHVVF
                     WAAMAFLGFLQITGALLNLLPVPGLDGYGALEPHLSPETRRALEPAKQWAFLIFLVLF
                     IATPLNRYFFAVVGWFFDLSGVNSGLIGLGFDLTRFWSAWL"
     gene            complement(2901..>3251)
                     /locus_tag="VGP27_12590"
     CDS             complement(2901..>3251)
                     /locus_tag="VGP27_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="retinol dehydrogenase"
                     /protein_id="HEV7852171.1"
                     /translation="LAVMLFTDELARRGVRAIVVDPGGADTNIVRYSTGFLRWSAGLS
                     LLRDFPQSAKSAARSSVMGATSDLPPATYLAPVFKQFGKPRVTRRLKKACDQETATRL
                     WKLSAELTECDWPH"
BASE COUNT          548 a         1107 c         1086 g          510 t
ORIGIN      
        1 gcgtgagtgt cggcccacgc ccggtcgtca tagttgacgt agctgtcgcg ggtggcgccg
       61 caggtgatgc tgcgcaaggt ggccaggtcg ccgttctgca cggcgacgtc gaagttctgg
      121 atggtggtgc ggaccatgtc ctgctgcgac tccttcgaat cggagccgcg ggtcagcagc
      181 agcgtgccca gtatcgcgac ggcggcgagc gccgctatca ccaggatcag cgccaccacc
      241 catccccagc tgcgacgccg gcccggcgga ttcggtgcgt ccccgcgccc cgggatcagt
      301 tgtggcgcaa ccgatttgcc ggagatgggc tgggtaggcg tggaaaagac ttcggtggcg
      361 gggtcgggag gcctttcgat gaatttcgtc gccgacgagt ccatcccgga cggcgcggtg
      421 taccgccgtt cggcgggctc ctccggctgc gtatccggct ccagcgcacc ggtgtcctcc
      481 accgccatca cttcggtggg gtgctcctcg acgatcggcc ccgcctcggc gctgactacc
      541 ggggccgcct cggtccgggc gctgtcctcg gttgtcgatg cgtcatcggc cggttcgccg
      601 gttgattcga cggccggctg gtgtccctgg ccgggccctg atgggttcga catccgtgca
      661 gcggtcctcc cgtgacgaac tggctgtcgg ctcggccgac ggtctctgca tcgagcttaa
      721 ttgccccacg gcacaatagg cctatgacgc gtatgggtga tctcctggga ccggatccga
      781 ctctgctgcc cggcgatccc gccgccgagg cggaactcga tgccgacgag aagcccgcga
      841 tcgtggccgc cgcccatccc accgcatcgg tggcgtgggc ggtgctcgcc gagcgtgcgc
      901 tggcagacga caaggccatc accgcctacg cgtacgcccg caccggctac caccgcggcc
      961 tcgaccagtt gcgccgcaac gggtggaagg ggttcggacc ggttccgtat tcgcatgcgc
     1021 ccaatcgcgg cttcctccgg tgcgtcgccg cactcgcgaa ggccgcggat gcgatcggcg
     1081 agtcggacga atactcccgc tgcctcgacc tgctcgacga ctgcgatccg agcgcccgcg
     1141 ccgaactcgg cgtcggctag ctctaccagc tcgtttcgca gtggcagtcg cccgcggcgt
     1201 cgggcggctc gacctggacg gtcgcatggt ccagaccgcg ggcgatcagc accgccttgg
     1261 cctcgtcgag gaccctcgcc gagtcggctg tcgacgtcag gtgtgcggtc accatgtcct
     1321 tgccgggaac cagcgtccag acgtgcaggt cgtgcacctc ggtcacgccc gcgaccgcgc
     1381 acagtgcgat ccgcagctcc gcgacgtcga tgtggcgcgg cgaattctcc gaaaggatgc
     1441 gcaacgccgc ccgcgcgagc gcgatcgctc gcggcagtac ccacagcgcg acgaataccg
     1501 cgaccacgac gtcggcgtag ggccaccggg tggtgaccgt gacgattccc gcgatcagca
     1561 cgccgaggct gccgacggtg tcggcgacga cctccatgta ggcgcccttg accgcgaggc
     1621 tctgcccgga ctgtgagcgc aacagcagca tcacgatcgc gttggcgatc aggccggcga
     1681 gcgcgacgac gatcatcggc acaccgggga cgtcgggcgc atcgccgagc cgctcgaacg
     1741 cctcgtagag gatgaagacg gcgacgaaca acagcagcac cgcgttggcc accgcggtga
     1801 acacctcggc gcgatgccag ccgtaggttc gcttggcgga agcgctgccg tgccgggcca
     1861 gcagcaccgc ggtcaggccc atgaacatgg ccaccaggtc ggtgagcatg tggcccgcgt
     1921 cggccagcag tgcgatcgag ttgatcagca gcgctgtcgt cagctcgatg acgaagaaga
     1981 ccgacaggac ggccgccgcg atgatcatcc ggctgacgcg ggtatcgcca cttccgtggt
     2041 tgtggcccgc acccatatct tcaatgtatg cgcaacgacg catatgtcgc aaggcgctgc
     2101 tttacagcca ggccgaccag aaccgggtga ggtcgaagcc gagtccgatc aggccgctgt
     2161 tgaccccgga caggtcgaag aaccagccca ccaccgcgaa gaagtaccga ttgagcggcg
     2221 tcgcgatgaa cagcaccagg aagatgagga acgcccactg cttggcgggc tccagtgcgc
     2281 gccgcgtctc ggggctcagg tgcggttcga gtgcgccgta gccgtcgagt ccgggtaccg
     2341 gtaggaggtt gagcagggct ccggttatct gcaggaagcc caaaaacgcc atggcggccc
     2401 agaacaccac atgttcgggg ttgtagaaca gccgcgtagc caccagcagc agcaccgcga
     2461 gcaccgcgtt catgaacggc ccggcgaggc tgacgatcgt cttctgcctg tcggtcatga
     2521 acgacgtccg cacccacact gcgccgccgg gcagcccgat tccgcccagc agagtgatca
     2581 acaccggaag tatcagggaa agcatcggat tggcgtactt gaacgggttg agcgtcagat
     2641 agccgcgcac cgcgatgtcg tggtcgccgt accgccacgc cgtgaacgcg tggccgaact
     2701 cgtggaagca caacgacacg atccagccgg cgatgacgaa gacgaacacg cccatgtagg
     2761 ccagcggcgt acgcgaggac tgggccagcc aggccagcac cgctccggcc gccgtgaccg
     2821 cgacgacgat caggaacatc gggcttggcc gcaccgattg gtgagccggg cggattgtca
     2881 cctcacgaaa ctaccggtgt ctaatgcggc cagtcgcatt cggtcaattc ggcagacagc
     2941 ttccacagcc gggtcgcggt ctcctgatcg cacgccttct tcaaccgcct ggtcacccgc
     3001 ggcttgccga actgcttgaa caccggcgcc agatacgtgg cgggcggcag gtcgctcgtc
     3061 gcgcccatga cgctcgagcg cgctgcggac ttcgcgctct gtgggaagtc ccgcagcagg
     3121 ctcagacccg cggaccagcg gaggaacccc gtgctgtacc gcacgatgtt ggtgtcggcg
     3181 ccgcctggat cgaccacgat cgcccgcacg ccccttctgg ccagttcgtc ggtgaacagc
     3241 atcaccgcga g
//
LOCUS       DASYLX010000519         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2986439,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000519 DASYLX010000000
VERSION     DASYLX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986439"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VGP27_12595"
     CDS             complement(<1..1381)
                     /locus_tag="VGP27_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEV7852172.1"
                     /translation="MTPAITVLAADTELAEPVAAGWQLVLAALAGIALIVVLITVAKL
                     HPFLALIFGGITVGIVAGVNMGDVLESFADGFGTTAAGVGVLIALGAMFAKLLADSGG
                     ADEIVDTIVGHASPRALPWAMALVGAIIGLPMFFEIGLVLLMPVIYLVAKRSQLSLVT
                     VGIPALAGLSAMHGLVPPHPGPLTAIDLLGADLGITLALGVAVAIPTVIVAGPLFGRL
                     AGRWVVLDVPDRFDADDFGGNGGAGAAAGEVEAGGAGAATQTATRTQRQRPSFGVTLF
                     SVLLPVALMMGKALVDIFIDDENAVLRQTFDIIGRPLMALLIAVVVGMFTLGRGAAMT
                     RDQIAKCLETSLPPVAGIILIVAAGGGFKQVLVDTGIGTKLAEWATAANISTILLAWV
                     LAVLIRLATGSATVATITASSLMLGLVEGMSTGQVSLIVLAVGAGSVFFSHVNDAGFW
                     LVKEYFGMSV"
     gene            complement(1378..>1745)
                     /locus_tag="VGP27_12600"
     CDS             complement(1378..>1745)
                     /locus_tag="VGP27_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV7852173.1"
                     /translation="NDDDRFPWLEAIGQWLGVRCKDGGVMSCSALKHKYRDQLRRHCP
                     DVIFLHLAGSPEVIGRRQASRPGHFMPASLVASQFQTLEPLAADEHGVTIDVDQDIDS
                     IVETYLALSASRATEQETK"
BASE COUNT          329 a          597 c          556 g          263 t
ORIGIN      
        1 ccacactcat cccgaagtac tccttcacca gccagaagcc ggcgtcgttg acgtgggaga
       61 agaagaccga gcccgctccg acggcgagca cgatcagcga cacctgtccg gtgctcatcc
      121 cctcgaccaa cccgagcatc agcgaggatg cggtgatcgt ggcgaccgtc gcggaacccg
      181 ttgccagccg gatcaatacg gcgagcaccc aggccaacag gatggtcgag atattggccg
      241 cggtagccca ttcggccagt ttcgtaccga tgccggtgtc aacgaggacc tgcttgaacc
      301 cgccgcctgc ggccacgatc aagatgatgc ccgcgacggg gggcaacgat gtctccaggc
      361 acttggcgat ctggtcacgc gtcatggccg caccgcgacc gagcgtgaac atgccgacca
      421 cgaccgcgat caacaacgcc atcaggggac ggccgatgat gtcgaaagtc tgcctcagca
      481 cggcgttttc gtcgtcgatg aagatgtcga cgagcgcctt gcccatcatc aacgccaccg
      541 gcagcagaac cgagaacagc gtcacgccga acgacgggcg ctggcgttgc gtgcgcgtgg
      601 cggtttgcgt cgccgctccg gctccgcccg cttccacctc acccgcggcg gccccggctc
      661 ccccgttacc gccgaagtcg tcggcgtcga accggtcggg cacatccaga accacccagc
      721 gccctgccag acgtccgaac agcggcccgg ccacgatcac tgtcggaatg gcgacagcga
      781 caccgagcgc cagcgtgatg ccgaggtcgg cgccgagcag gtcgatggca gtgagcggcc
      841 cggggtgcgg cggcaccagt ccgtgcatgg cggaaagccc tgccagcgcc ggtattccga
      901 ccgtgacgag tgacagctgc gagcgcttgg ccaccagata gatcaccggc atcagcaaca
      961 ccaggccgat ctcgaagaac atcggcaggc cgatgattgc gccgaccagc gccatcgccc
     1021 atggcagcgc ccttggcgac gcgtggccga cgatggtatc gacgatctcg tcggcgccgc
     1081 cggagtcggc cagcagtttg gcgaacatag ccccgagtgc gatcaacacc cccaccccgg
     1141 ccgccgtcgt tccgaatccg tcggcgaacg attccagaac atcgcccatg ttgaccccgg
     1201 ccacgatgcc gacggtgatg ccgccgaaga tcagcgccag gaacggatgc agcttggcga
     1261 cggtgatcag cacgacgatg agtgcgatgc ccgcgagtgc ggcgagcacc agttgccagc
     1321 cggccgcgac gggttcggcc aactcggtgt cggccgccaa gaccgtgatt gccggggtca
     1381 tttcgtctcc tgttcggttg cgcgcgaggc ggataacgcg agataggttt cgactatcga
     1441 gtcgatgtcc tggtcgacgt cgatggtgac gccgtgttcg tcggccgcga gcggctcgag
     1501 tgtctgaaac tgcgaagcca ccagcgacgc gggcatgaag tgccccgggc ggctggcctg
     1561 acgcctgccg atcacctccg gtgacccggc gaggtgaagg aagatgacgt ctggacaatg
     1621 ccgccgcagc tggtcgcgat acttgtgttt cagcgccgaa caactcatca cgccgccgtc
     1681 tttgcagcgc acgccgagcc actgcccgat cgcctccagc cacgggaacc ggtcgtcgtc
     1741 gttca
//
LOCUS       DASYLX010000520         4670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3534824,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000520 DASYLX010000000
VERSION     DASYLX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3534824"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP27_12605"
     CDS             complement(<1..306)
                     /locus_tag="VGP27_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852174.1"
                     /translation="MATNVPPRRPVNGKQLRADRTREMVIDETVRCVIEEGFNAASAK
                     HITERAGVTWGVIQYHFGDRDGLLMAVVDKGFGQLLETLRGMEPELASTAGHARAELV
                     "
     gene            complement(327..878)
                     /locus_tag="VGP27_12610"
     CDS             complement(327..878)
                     /locus_tag="VGP27_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852175.1"
                     /translation="MTRSDWLVGGDRRTAAAERIYAVATDMIARDGMDAFDIDALASR
                     VHCSRATIYRCTGGKRQIRDAVMERSAGRIVDVVRKAVDGLAGAQRIVTAITVALEEI
                     RSDPLGQLMFSAIRGAEERAWLTESPVVARFATDLNGLTDDDTEAAQWIVRIVLSLLY
                     WPVGDADVERQMVKRFISPAFAP"
     gene            1042..2259
                     /locus_tag="VGP27_12615"
     CDS             1042..2259
                     /locus_tag="VGP27_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852176.1"
                     /translation="MPVRADQLGIELFEDEFVQDPYPLYERMHAAGPVHPIADSGFHA
                     VSGWDAVNDVIARPEDFSSNLTATMTYQGGKVGAFPMGELGGEIHALATADDPAHATH
                     RKLLVPQLAARRINALEPFIEETNRRLWDEGVQAGVIEWMTAMANRLPMMIVTRLIGV
                     PDVNIEQLVRWGYASTQLVEGLVDQNRLEEAGVAVMELAGYIGEHFARAAADPHDDLL
                     SDLATDCASGELGELAAMTMMITLFSAGGESTASLIGSAAHALADHLDVQEQVRENPE
                     LLGTFLEEVLRYEPPFRGHYRHVLNDTTLCGVDLDAGSRLVLLWGAANRDPSHFVNAN
                     EFRLDRTGGKGHISFGKGAHFCVGAALARLEARVVIGELLKRTSHIEATDTGRWLPSL
                     LVRRLERLQLRVA"
     gene            complement(2266..3354)
                     /locus_tag="VGP27_12620"
     CDS             complement(2266..3354)
                     /locus_tag="VGP27_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7852177.1"
                     /translation="MSRTDKPIRVFQVATGNVGSEMIKRIADQPDLELIGVHCYSPEK
                     VGKDAGALAGSAPNGVKATGTVDEIIAAKPDVLTFHGVFPDEDLYVKVLEAGIDIVTT
                     ADWITGWHRDTNHPHPSGKPVSALLADACEKGGSTFYGTGMNPGLNQILGVVCSADVA
                     EIENVTTIESVDVSCHHSKDTWIEVGYGLPVADPSIPGKLERYTRVFADSVLMMADCF
                     DLELDEVKFTYELGACTKDVDLGWYTLPKGSLGGNYIRYQGMVDGVARVETHLEWQMT
                     PHTDPSWDIKGCYITAIKGDPCVYNKHMIFPKPGVDLSNPDNFASIGMTVTGMPALAA
                     IRSVVAAPPGLVTSADLPLRGFAGRFKL"
     gene            complement(3351..4379)
                     /locus_tag="VGP27_12625"
     CDS             complement(3351..4379)
                     /locus_tag="VGP27_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7852178.1"
                     /translation="MAKPPLSMKPTGWFQVAWSDEIKLGDVHRMKYFGRDLVAWRAQS
                     GQLTVMDAYCGHLGAHLGYGGHVVDEVLQCPFHGWQWNQDGRNVCIPYQDRPNRGRRI
                     RTFPVVERNESAYIWHDVEGRDPYFEAPDIFAGFSDGSAAADYYPQQRLYRETLEMHP
                     QYVLENGVDFAHFKYVHQTPIVPVFTRHDFDEPVSYVDFTITFEGDENQTIDDVKSGV
                     EAINGGLGIAVTKSWGMVDNRTISAITPVDENTSDVRFMVYIGRRAGDDSPKAEAKAR
                     DFGHEIIRQFTQDIHIWAHQRYSDPPALAGAEQEGFTAIRNWATQFYPDGIGDSAAEV
                     YAQKGRTE"
     gene            4492..>4670
                     /locus_tag="VGP27_12630"
     CDS             4492..>4670
                     /locus_tag="VGP27_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7852179.1"
                     /translation="MARAARWSAVGFIAALIFALVACSTGERVDLGGDSSGRLVAAIA
                     GEPDQLDPNKTSAYFS"
BASE COUNT          846 a         1477 c         1459 g          888 t
ORIGIN      
        1 caccagctcg gccctcgcat ggccggccgt cgaggccaat tccggttcca tgccgcgcag
       61 tgtttccagg agttggccaa accccttgtc caccacagcc atcagcaggc cgtctcggtc
      121 gccgaagtga tactggatca ctccccacgt gacacccgcc cgctcggtga tgtgcttggc
      181 gctcgcggcg ttgaaaccct cctcgatgac acaccgcacg gtttcgtcga tgaccatctc
      241 ccgcgtgcgg tccgcacgga gttgttttcc gttgacgggt cgacgtggcg gaacgttggt
      301 ggccatggtt gtagattcga ttctattcat ggagcgaacg caggagagat aaagcgcttc
      361 accatctgac gctcgacgtc tgcatcgccg acaggccagt acaacagcga cagcacgata
      421 cggacgatcc actgagcagc ctcggtgtcg tcgtcggtca acccgttgag gtcggttgcg
      481 aagcgggcca cgactggaga ttcggtgagc caggcccttt cctctgcacc ccgaatcgcg
      541 ctgaacatca actggcccag cgggtccgat cgaatctctt cgagcgccac cgtgatcgcg
      601 gtgacgatcc gctgcgcgcc ggccagcccg tcgacggcct ttcgcaccac gtcgacgatc
      661 cgacctgccg agcgctccat cacggcgtca cggatctggc gcttgccgcc ggtgcagcgg
      721 tagatcgtcg ctcgggaaca gtgcacccgg gatgccagtg cgtcgatgtc gaatgcgtcc
      781 atgccatccc gcgcgatcat gtccgtcgcg acggcataga tgcgctcggc cgcggcagtg
      841 cgtcgatcac caccgacgag ccagtcgctg cgagtcacag cctcaacttt ctcaccttga
      901 gacgtatttg gccagaattc tcaccgctgt tgcaggtcgg aggcgtggcg tgagacgacc
      961 cgccacatgc cgatcgacgt gcacttttca acttcacgcc caccgttgac gcgccgctgc
     1021 cgccccgatc agccttgagg gatgccggtg cgcgcagatc aacttggtat cgagctattc
     1081 gaggacgaat tcgttcagga tccctatccg ctgtatgagc gcatgcacgc ggcagggccg
     1141 gttcacccca tcgcagactc cgggttccac gcggtgagtg gttgggacgc ggtcaacgac
     1201 gtcatcgccc ggccggagga cttctcctcg aacctgaccg ccacgatgac ataccaaggc
     1261 ggcaaggtgg gcgcctttcc gatgggcgaa ctcggcggcg agattcatgc cctcgcgact
     1321 gcggacgatc cagcgcacgc tacgcaccga aaactgttgg tgccgcaatt ggcggcgagg
     1381 cgaatcaacg cgctcgaacc gttcatcgaa gaaaccaatc gccgattgtg ggacgaaggc
     1441 gtgcaggccg gtgtcatcga atggatgacg gcaatggcca accggctgcc gatgatgatc
     1501 gtcacgcggc tcatcggtgt gcccgacgtg aacatcgagc agctggtgcg ctggggctac
     1561 gccagcaccc aactggtgga aggactcgtc gaccagaacc gactcgaaga ggcgggcgtt
     1621 gcggtcatgg agctcgccgg ctacatcggt gaacactttg cgcgggcggc cgccgacccg
     1681 cacgacgatc tgctcagtga cctcgcaacg gactgtgcat caggcgaatt gggggaactg
     1741 gcagcgatga ccatgatgat cacgctgttc agcgccggtg gcgagtcgac cgcatcgttg
     1801 atcgggtccg ctgcccatgc cttggcggat cacctcgacg tccaagagca ggtgcgcgaa
     1861 aaccccgaac tcctcggcac tttcctcgaa gaggtgcttc gctacgaacc gccgtttcgg
     1921 ggacactatc gccacgtgct caacgacacg acgctctgtg gtgtggatct cgacgccgga
     1981 tcacgtctcg tgcttctgtg gggcgcggcc aaccgggatc cctcacattt cgtgaatgcc
     2041 aatgagtttc ggctggaccg cacgggcggg aaggggcaca tcagcttcgg aaagggagcg
     2101 cacttctgtg tcggggcggc actcgcgcgg ctggaggcca gggtcgttat cggcgagctc
     2161 ctcaagcgca cgtcgcacat cgaggcgaca gacaccggac ggtggctgcc gagcctgctc
     2221 gtgcgcaggc tcgaacggct acagctgcgc gtcgcctgag ctgcgtcaga gcttgaagcg
     2281 accggcgaat ccgcgcagcg gcagatccgc gcttgtcaca agtcccggcg gggcggcgac
     2341 caccgacctg atcgccgcaa gggcgggcat cccggtgacc gtcataccga tcgacgcgaa
     2401 gttgtccgga ttcgacaggt cgacgcccgg cttcgggaag atcatgtgct tgttgtagac
     2461 gcacgggtcg cccttgatcg ccgtgatgta gcagcccttg atatcccagc tcggatccgt
     2521 gtgcggagtc atctgccact cgaggtgtgt ctccaccctc gccacaccgt cgaccatgcc
     2581 ctggtaacgg atgtagttgc cgccaagcga tcccttgggc agcgtgtacc agcccaggtc
     2641 gacatccttg gtgcacgcac ccaactcata ggtgaacttg acctcgtcga gttccaggtc
     2701 gaagcagtcc gccatcatca gcacgctgtc ggcgaagacg cgggtgtagc gctccagctt
     2761 ccccggaatg ctcggatcgg cgacgggcaa cccgtaaccg acctcgatcc aggtgtcctt
     2821 gctgtggtgg caggaaacgt cgacggattc gatcgtcgtg acgttctcta tctcggcgac
     2881 atcggccgaa cagaccacac cgagaatctg attcaagccg ggattcatgc ccgtcccgta
     2941 aaacgtcgag ccacccttct cgcaagcgtc agccagcaac gcagacaccg gcttgccgga
     3001 cggatgcgga tggttggtgt cgcgatgcca gccggtgatc cagtcggccg tggtgacgat
     3061 gtcgataccc gcctcgagga ccttcacata cagatcctcg tcggggaaga caccgtgaaa
     3121 cgtcagcacg tcgggtttgg ccgcgatgat ctcatcgacg gttccggtgg ccttcacacc
     3181 gttcggcgcc gagccggcca gcgcgcccgc gtccttgccg accttctccg gcgagtagca
     3241 gtgcacaccg atcagttcca gatcgggctg atcggcgatg cgcttgatca tctccgaacc
     3301 gacgttgccc gtcgcgactt gaaagacacg aatcggcttg tctgtgcggc tcattcggtt
     3361 cgacctttct gtgcgtacac ttcggcggcg ctgtcaccga tcccgtcggg atagaactgc
     3421 gtggcccagt tgcggatggc ggtgaagccc tcctgctcgg cgcctgccag cgcgggcgga
     3481 tccgaatagc gctggtgcgc ccagatgtgg atgtcctggg tgaactgccg gatgatctcg
     3541 tgtccgaaat cgcgcgcctt ggcctccgcc ttcggcgagt cgtctcctgc gcggcggccg
     3601 atgtagacca tgaaacggac atcggatgtg ttctcgtcta ccggcgtgat ggccgagatg
     3661 gtccggttgt cgaccatccc ccagctcttc gtcaccgcga ttcccagacc gccgttgatg
     3721 gcttcgacgc cacttttgac gtcgtcgatg gtctggttct cgtcgccttc gaaggtgatc
     3781 gtgaagtcga cgtaggacac cggctcgtcg aaatcgtggc gggtgaagac cggaacgatc
     3841 ggagtctggt gcacgtactt gaaatgtgcg aagtcgacgc cgttctcgag gacgtactgc
     3901 ggatgcatct ccaacgtttc gcggtacaac cgctgttggg gatagtagtc ggcggcggcg
     3961 ctgccatcgg agaagcccgc gaagatgtcg ggtgcctcga agtaggggtc gcggccctcg
     4021 acgtcatgcc agatgtaggc cgactcgttg cgttcgacca ccgggaacgt gcggatgcga
     4081 cggccgcggt tcggtcggtc ctgatacggg atgcacacgt tgcggccgtc ctgattccac
     4141 tgccaaccgt ggaacgggca ctgcaggacc tcgtccacca catgtccgcc atagccgagg
     4201 tgcgcgccaa gatgtccgca ataggcgtcc atgacggtca gctgtcctga ttgcgcacgc
     4261 cacgcgacga ggtctctacc gaagtacttc atccggtgca cgtcgccgag cttgatctcg
     4321 tctgaccagg cgacttggaa ccatccagtc ggcttcatcg acaaaggcgg cttggccatg
     4381 actcgaaatc atagaggatt caatgtaaag ggtgaaaccc cgaggttgcg cgtttcgatc
     4441 accgtgcaac ccgggtagta gtcgaagggc cgacgattcg gggtgatggt gatggcgaga
     4501 gcggcgaggt ggtccgccgt tggcttcatc gccgcactga tcttcgccct cgttgcctgt
     4561 tcgacgggcg aaagggtcga tctcggcggt gactcgtcgg gacgcctggt ggcagcgatc
     4621 gccggggagc ccgaccaact cgaccccaac aagaccagcg cctacttctc
//
LOCUS       DASYLX010000521         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2109990,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000521 DASYLX010000000
VERSION     DASYLX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109990"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VGP27_12635"
     CDS             complement(<1..87)
                     /locus_tag="VGP27_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7852180.1"
                     /translation="MAYLKPPWFVRKIFNRVAMATGVGNSQTL"
     gene            complement(145..1494)
                     /locus_tag="VGP27_12640"
     CDS             complement(145..1494)
                     /locus_tag="VGP27_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852181.1"
                     /translation="MTDISALPLAPKNPLPLKDLVKLLRRLDTGQETIRDAGGPITRI
                     QFGPKKLFPPIVAVFSPNAMRDVLGRTDAFSERCIIHNEVRDIAGDSLFVLPNEQWRP
                     RKRALQPVFTKHNVRTFGGHMSGAAQKFVDRWLGGAEIDLDVESRKVAMASLGRSILG
                     VDLNERADTIARCMHVASSYSADRALRPVRAPRWLPTPARRRARQAVAAMHAITDQMI
                     AACRAEPNREAPLVQALIAATDPQTGQSLSDADISNDLLLFMLAGHDTTATALTYSLW
                     VLGHHPDIQERVAAEAAAIGDRELTPEDVPHLGYTTQVLHEALRMCPPAAGVARLATR
                     DIAVDGYRVEAGTLIAVGLYGLHNDPALWDRPSDFDPDRFSPENNKARDRWQFLPFLG
                     GGRPCIGEHFARLETTLALATIVRAMEIRSVDDDFPVEVPFTTVAKGPIRAHVTART"
     gene            complement(1491..>1714)
                     /locus_tag="VGP27_12645"
     CDS             complement(1491..>1714)
                     /locus_tag="VGP27_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852182.1"
                     /translation="RMADALREIFPEGFADRIGEPAVNDLADTILRYAAMALLLPGAQ
                     PLDSVDDIRAFATRHFLPSLSTALRAVPA"
BASE COUNT          287 a          549 c          590 g          288 t
ORIGIN      
        1 gagtgtctgg ctgttgccga cacccgtcgc catcgcgacc ctgttgaaga tcttgcggac
       61 gaaccacgga ggcttgaggt aggccatagc cccgagtttg gcggtggtgt gcaagtaccg
      121 caatgggtgg cgcaattcaa cacgtcacgt ccgcgcggtg acgtgcgcgc ggatcgggcc
      181 cttggcgacc gtggtgaacg gcacctcgac gggaaagtcg tcgtcgaccg acctgatctc
      241 catcgcccgc acgatggtgg ccagcgccag ggtcgtctcc aaccgcgcga agtgttcacc
      301 gatgcacggg cgcccgccgc ctagaaacgg caggaactgc catcggtccc tggctttgtt
      361 gttctcaggg ctgaaccggt ccggatcgaa gtccgacggc cggtcccaca gcgccggatc
      421 attgtgcagc ccatagagtc ccacggcaat cagggtgccc gcttccacgc ggtagccgtc
      481 gacggcgatg tcgcgagtgg ccaggcgcgc cacacccgcc gcgggtggac acatccgcaa
      541 cgcctcgtgt agcacctgcg tggtgtagcc gaggtgcggc acatcttcgg gggtcagctc
      601 acggtcgccg atggcagcgg cctcggcggc gactcgctct tggatgtcgg ggtggtggcc
      661 gaggacccac agggagtacg tcagcgctgt cgccgtggtg tcgtggccgg cgagcatgaa
      721 caacagcagg tcgttggaga tgtcggcgtc ggaaagcgac tgcccggttt gaggatcggt
      781 ggcggcgatc aacgcctgta ccaacggcgc ctcgcgattc ggctccgcgc ggcaggcagc
      841 gatcatctgg tcggtgatcg catgcatcgc tgcgaccgcc tggcgggccc ggcgccgggc
      901 aggggtgggg agccatctcg gcgccctcac cgggcgcaat gcgcggtcgg cggaatagga
      961 cgatgcgacg tgcatgcacc gggcgatcgt gtctgcccgt tcgttgagat cgacgccgag
     1021 aatcgagcgg cccagcgaag ccatggccac cttgcgagac tccacatcca ggtcgatttc
     1081 tgcgccaccc agccagcggt cgacaaattt ctgtgcggca ccggacatgt ggccgccaaa
     1141 agtgcgcaca ttgtgctttg tgaagaccgg ctgaagcgct cgtttgcggg gccgccattg
     1201 ctcgttgggc aacacgaaaa ggctgtcgcc cgcgatgtcg cgcacctcgt tgtggatgat
     1261 gcaccgctcg gagaaggcgt cggtgcgtcc taacacgtcg cgcatcgcgt tcggcgagaa
     1321 caccgccacg atcggcggga acaacttctt cggcccaaac tggatgcggg tgatcggtcc
     1381 gcccgcgtcg cggatcgtct cctggcctgt gtcgagccgc cgtagcagct tcaccagatc
     1441 cttcagaggc aaaggattct tcggggccag cggcagcgcg ctgatgtccg tcacgcgggg
     1501 accgcccgca acgccgtcga caggctgggc aggaagtgcc gggtcgcgaa ggcgcggatg
     1561 tcgtccacgg agtcaagcgg ttgcgcgccg ggcagcagca gcgccatcgc cgcataccgc
     1621 agaatcgtgt cggccaggtc gttgaccgcc ggttcgccga tgcggtctgc gaacccctcg
     1681 ggaaagatct cacgcagcgc atcggccatc cgct
//
LOCUS       DASYLX010000522         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2766601,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000522 DASYLX010000000
VERSION     DASYLX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2766601"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VGP27_12650"
     CDS             <1..1178
                     /locus_tag="VGP27_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852183.1"
                     /translation="TLGLTEFQTEVIANVRRFVDKEIIPHAQELEHADTYPHEIVEQM
                     REMGLFGLMIPEEYGGLGESLLTYALCVEELARGWMSVSGVINTHFIVAYMLRQHGTE
                     EQRQRFLPRMATGETRGAFSMSEPELGSDVAAIRTRAKRNDDGDYTIDGQKMWLTNGG
                     SSTLVAVLVRTDEGAEKPHRNLTAFLVEKPTGFGEVAPGLVIPGKIDKLGYKGIDTTE
                     LIFDGYVAKADDILGETPGQGFFQMMDGIEVGRVNVSARACGVGIRAFELAVKYAQQR
                     QTFGKPIAEHQAIAFQLAEMATKVEAAHLMMVNAARLKDSGERNDVAAGMAKYLASEF
                     CSEVTQQSFRIHGGYGYSKEYEIERLMRDAPFLLIGEGTSEIQKNIISKRLLADYRI"
     gene            1181..>1695
                     /locus_tag="VGP27_12655"
     CDS             1181..>1695
                     /locus_tag="VGP27_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7852184.1"
                     /translation="MTVPEFATRTQLSEAVARYVRKRIFDGTYAAAEYVRLDQLAAEL
                     GISVTPVREALFQLRAEGLLDQKLHRGFVVLPVTRRDITDVSDVQAHIGGELAARAAS
                     NITDDQLRELERLQRKLEDAYASDDEDHAVALNHEFHRAINVAADSPKLAQLMSQITR
                     YAPESVFPTIEG"
BASE COUNT          344 a          527 c          551 g          273 t
ORIGIN      
        1 agaccctggg tctgacggag ttccagaccg aagtcatcgc caacgtaagg cgattcgttg
       61 acaaggagat catcccgcac gcgcaggaac tcgagcacgc cgacacctat ccccacgaga
      121 tcgtcgagca gatgcgggag atgggtttgt tcggtctgat gattccggag gagtatgggg
      181 gactcggcga gtcactgctg acctatgcgc tgtgcgtcga ggagctcgcc cgcggctgga
      241 tgagcgtctc cggtgtgatc aatacccact tcatcgtcgc gtacatgctg cgccagcacg
      301 gcaccgagga gcagaggcaa cgcttcctgc cgcggatggc caccggcgag acgcggggtg
      361 cgttctcgat gtccgagccc gaactcggct ccgacgtcgc cgcgatccgc accagggcca
      421 agcgcaacga cgacggcgac tacaccatcg acggccagaa gatgtggctc accaacggcg
      481 gcagctcgac gctggtcgcg gtgttggtgc gaaccgacga aggcgcggag aagccgcacc
      541 gcaacctcac cgcgttcctc gtcgaaaagc caaccggttt cggggaagta gcgcctggat
      601 tggtgattcc cggcaagatc gacaagctcg ggtacaaggg catcgacacc accgagctga
      661 tcttcgacgg ctatgtcgcc aaggccgacg acatcctcgg cgagacaccg gggcagggct
      721 tcttccagat gatggacggc atcgaggtgg gccgcgtcaa cgtgtcggcg agggcctgcg
      781 gcgtcggcat ccgcgcgttc gagctggcgg tgaagtacgc ccaacagcgt cagaccttcg
      841 gcaagccgat cgccgagcac caggcgatcg cgttccagct cgccgagatg gctacgaaag
      901 tcgaggcggc acacttgatg atggtcaacg ccgcccggct caaggactcc ggcgaacgca
      961 acgacgtggc ggcggggatg gccaagtacc tggcgagcga gttctgctcg gaggtcaccc
     1021 agcagagctt ccgcatccac ggcggctacg gctactccaa ggaatacgag atcgaacggc
     1081 tgatgcgcga cgccccgttc ctgttgatcg gcgagggtac cagcgagatc cagaagaaca
     1141 tcatcagcaa gcggctgctc gcggattatc ggatctgact gtgaccgttc cggaattcgc
     1201 aacgcggaca cagctttccg aggctgtggc gcggtacgtc cgcaagcgga tcttcgacgg
     1261 gacgtacgcg gcagccgagt acgtgcggct cgaccagttg gcggccgaac tgggtatcag
     1321 cgtgacaccg gtgcgtgaag cgctctttca attgcgcgcc gagggtctgc tcgaccagaa
     1381 gctgcaccgc ggattcgtgg tgctgccggt gacccgtcgc gacatcaccg acgtctccga
     1441 cgtgcaggcg cacatcggcg gcgaactggc cgcgcgtgcc gcctccaaca tcaccgacga
     1501 ccagctgcgc gagctggaaa gacttcagag gaaactcgaa gatgcctacg cgagcgacga
     1561 tgaggaccat gcggtcgccc tcaatcacga gttccaccgg gcgatcaacg tggctgcaga
     1621 ttcgcccaag ctcgcgcagt tgatgtcgca gatcacgcgc tatgcacccg aatcggtttt
     1681 tccgacgatc gaagg
//
LOCUS       DASYLX010000523        20568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3864251,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000523 DASYLX010000000
VERSION     DASYLX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864251"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGP27_12660"
     CDS             <1..715
                     /locus_tag="VGP27_12660"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV7852185.1"
                     /translation="IERGDYPKWGVYIQVMPEADAATYRFHPFDLTKVWSQKDYPLIE
                     VGEYELNRNPDNYFADVEQAAFTPANLIPGISFSPDKMLQGRLFSYGDAARYRVGVNH
                     HQIPVNYPRAAKVVNAYHRDGSMRVDGNQGGVAGVEPNNFGRWAEQPAYADPAESIGS
                     TADHFDFREDDDNYFEQPGDLFRLMTGDEQQRLFDNTARAIKGARHQTVEMHIANCTQ
                     ADAAYGEGVRKACEALGAL"
     gene            814..1287
                     /locus_tag="VGP27_12665"
     CDS             814..1287
                     /locus_tag="VGP27_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV7852186.1"
                     /translation="MTDHFAEDPGCQLDPELVDLASRIFEMARNGDAATLAVYLDAGV
                     PVNLTNTNGDTLVMLAAYHGHESTVAALTARGAEVDRHNKRGQTPLAGAVFKNDTTII
                     ELLLRADADPLAGSPSALETAQFFKREELERQLRLHIDGAAAHRSSGESPLPLAK"
     gene            complement(1375..1695)
                     /locus_tag="VGP27_12670"
     CDS             complement(1375..1695)
                     /locus_tag="VGP27_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7852187.1"
                     /translation="MTDVADFIDTLQRVAKGASVVDPALVQELVSARRRDDPLAVLTA
                     REQEVLALMAEGRSNAGIGHRLWITEGTIEKHVRSILTKLNLPETGDDHRRVLAVITF
                     LETH"
     gene            complement(1714..2985)
                     /locus_tag="VGP27_12675"
                     /pseudo
     CDS             complement(1714..2985)
                     /locus_tag="VGP27_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
     gene            3515..5416
                     /gene="dxs"
                     /locus_tag="VGP27_12680"
     CDS             3515..5416
                     /gene="dxs"
                     /locus_tag="VGP27_12680"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV7852188.1"
                     /translation="MAVLGRIRGPRDLQGLSRDELTRLTDAIRAFLIEKVSVSGGHLG
                     PNLGVVELTMALHLVFESPRDALIFDTGHQAYTHKILTGRQDDFDHLRASGGLSGYPS
                     RAESEHDWVESSHASAALSYADGLAKAFELTGSKDRRVVAIVGDGALTGGMCWEAMNN
                     IAAGKSRRVIVVVNDNGRSYAPTIGGLAERLTSLRTRRRYGRLLALVKRELTGLPTLG
                     RSTYSVLHAVKEGLKDALSPQTLFGDLGIRYLGPIDGHDIFALEKALRIATDVDGPVI
                     VHTVTRKGMGYRPAEEDPADRMHACGPIDPRTGKPKSGADVTQWTDVFASELLRWGSR
                     RDDVVAITAAMAGPTGLAAFGKQFPNRMFDVGIAEQHAVTSAAGLALGGMHPVVAVYS
                     TFLNRAFDQVLMDVAVLKLPVTFVLDRAGITGSDGASHNGVWDLSLLGMVPGMRVAAP
                     RDAASLREQLGEALGVDDGPTAIRFPKGSVGADTKALERIEGVDMLCRPQDPDVLLVA
                     VGPLASVALRAAATLAKRGVAVAVVDPRWVLPVPEQVVKLAGNVQLVVTLEESGLHGG
                     VGSAVAARMREARIDTPVRSVGVPQRFLTHASRSEIHADLGLTAADLAANISFWVQRP
                     ADVDVNRGE"
     gene            5492..7768
                     /locus_tag="VGP27_12685"
     CDS             5492..7768
                     /locus_tag="VGP27_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7852189.1"
                     /translation="MLRGIARLAIAAPRRMIAVALLIMIGAGIFGIPVTKSLSAGGFQ
                     DPTSESWRAARLLSERFGQGDMWMVIAVTSDAGIASPAARAVSADLVRHLQASPHVAD
                     ISSAWTAPPPAAPALISKDGKTGLIIAGITGGENGAQKHAKQLADQLVYDRDGVTVRA
                     SGDAVTYVQVNSQSEKDLLRMELIAIPLSFVVLVWVFGGVLAAALPLTVGGFAILSSM
                     AVLRAISYATDVSIYALNLAAALSLALAIDYTLLIISRFRDELADGGTRNAALIRTME
                     TAGRTVLFSAMTVALSMVAMVLFPMYFLKSFAYAGIAVVGFAAVAAVMVTPAAIVLLG
                     DRLDSLDVRRLGRKVLRRPEPVQRPVEQMFFYRSTKFVMRHAIVVGLAVTAVLLILGA
                     PFLGIKWGLPDDRVLPDSASARQVGDQIRSAAFAVNFANNTTVVIPDLTGVTADELGR
                     YAAQLSQVADVSSVSAPGGTFVDGRLARPESAAAIKDGSAFLTVNSTAPLYSEASEIQ
                     LDRLHAVAGPGGQHVQMTGIAQINRDSGEAITSRLPTVVAVIAAITFVLLFLLTGSVV
                     LPLKALLLNILSLSATFGALVWIFQEGHLDAFGTTATGTLLASVPVLLFCIAFGLSMD
                     YEVFLVARIREYWLTSACPGADNDESVALGLARTGRVVTAAASVMVISFAALIAAQVS
                     FMRMFGVGLTLAVLVDATLVRILLVPAFMHVLGRANWWAPKPMARLHSRLGISEARAP
                     EPTEVTPKKTGQPVDIGS"
     gene            7806..9278
                     /locus_tag="VGP27_12690"
     CDS             7806..9278
                     /locus_tag="VGP27_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEV7852190.1"
                     /translation="MTNRYLEAALAPINEEYTLTDLEVTGTIPTYLDGRYLRNGANPI
                     SEVDPELYHWFTGDGMVHGIRIRDGRAEWYGNRWVRGPLAKSKLGEAPPGGHFGLYGI
                     GANTNVIGHAGKTIALMEGGLANYELTDDLDTVGVCDFDGTLTGGYTAHPKRDPETGE
                     LHAVSYRWNRGNTVQYSVIGIDGRARRTVDIEVTGSPMMHDFSLTKRHVVFYDLPVTF
                     DVGRAAAMAVPRGLRLPARLVLSALIGRIRIPDPIAARMPCGTDSDRRLPYSWNPDYP
                     ARVGVMPRDGSNGDVRWFDVEPCYVYHPMNAYDDGDTDIVLDVVRHPKMFDTDHSGPN
                     EGPPTLDRWTVDLVGGKVRESRVDDRGQEFPRVDERVVGKPHRYGYAPTVGEDGTAGD
                     SLIKHDLVGENTQSRSFSADKMLGEFVFHPSCPDAEEDDGVLMGYLYDRPTDRSELAV
                     LDARTLQDVASVKLPHRVPAGFHGNWIPTQAGFSDVTTAG"
     gene            complement(9384..10400)
                     /locus_tag="VGP27_12695"
     CDS             complement(9384..10400)
                     /locus_tag="VGP27_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV7852191.1"
                     /translation="MTRRLTLPSATASVAPSERMPFSRRVLKLEHPANAGPLAHFAGW
                     FGLMAFALLVPAATNWYLAVPLIVLLALANLSITIGVLHMHTHRPLFVSPRMNRVVDV
                     LCCMPATLTAAEMREVHVLNHHRYNDGPGDVTSTEGREQGLRAVWYWISYGIIVKNHT
                     VRTTFAANASPGRRKRRYQFMLDYTLVVALMAATWYVTDTTRFTLFYAIPFIVTQVNS
                     GYFAWLTHAPARGFEEDPSKSMNTVGNWLNFFIFNQGYHSVHHRYPGIHWSQIPDKLD
                     FMQKVEPDVIVPYWMTLNSIWRLIVPGGFLTAAYGERWKAKLDKRIQEGTVRSRYLPW
                     FAWI"
     gene            complement(10397..11686)
                     /locus_tag="VGP27_12700"
     CDS             complement(10397..11686)
                     /locus_tag="VGP27_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852192.1"
                     /translation="MSGGRLGTLADGLRFLHRAPDLDAMRAARSPGELARLALIPAAR
                     NLGIAAGFLPTDLRTEATAALLACRVLDAYEDLSERPLAAGAVVTAVDYLNGDTDTLP
                     PPLQAIAVRDSEAVDLVLAERIRDVRALLSELPVEGRKRVGRMLVDVGHVMAHNLDSP
                     LPRTAYGEGVLGRVVLYVCSLVAEDACAQTDLSELAGCIGVTAQLANDLRDGELALYD
                     AGDREELTRAVMLRLLAPALGGFALLTRLGPCTPSRGARVAMAYMTITTTAFLCASVG
                     APAPYRRRLQLAAAMLAAHSPVHWARMLEQVRRSADRAIHRLLDASPNPLAEAGVATG
                     SGPAADVLRLGNPRSMSPSMGPLIVGATFALVDALPEEPLTGELPESQVRRMMIADHL
                     TFGALERLRPRDADAMQALATQLQLTALGAATQGGRP"
     gene            complement(11683..12852)
                     /locus_tag="VGP27_12705"
     CDS             complement(11683..12852)
                     /locus_tag="VGP27_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7852193.1"
                     /translation="MSSTAPKSVILQWQATALVAPHGSNDLLCGAKHLTRWPSFRQDI
                     AEGTMMGEQSASVASVGRSVGRPSLTGPSDQLTLVADRLHEILVSDGGFDVIYRHILA
                     VPGKRVRAAMVLACARLLPSAAAAPLRDAVDVACAIEMFHEASLVHDDICDGSLLRRD
                     APSVPAAFGVRTAARAGFHLAGKALQVLARVLADNPAVFARLGEAPGVTYLDRISELS
                     FGQLVETLPPAVDDAALRRHYRVVAGAKTGTLFRLACSYGGTAGAVDHDRLCVLMQYA
                     DQLALAFQIMDDVRDVEGGPSLGKEAGGDLDRRVPTWPVIEWLATRPDSHDMWLSGLT
                     SSADLQADLVHSGATRAARAVAVRAAERARRTMDVFPPSPAREHLRELAVRVVFR"
     gene            complement(13057..13848)
                     /locus_tag="VGP27_12710"
     CDS             complement(13057..13848)
                     /locus_tag="VGP27_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7852194.1"
                     /translation="MLSSKPYQPHTQSGRGATIADRSFLDFSVEELRSLIDTYQWLVF
                     KHHSLSGSDVAGHLRKFGRLVQNDRRQNGILKLDGSKKEEVLLGEGFMPLHRDGAFMG
                     NDIAFVGICCVEYQNVTGGGRTFISDIASAVKEVPGAMLDIIREKGIEGKPVDNYYTK
                     PSDAWLPIPGFIDVGGVSYLNVIFPYGPGEKPSWLIRIPGVEEELFQTIIETLSAALI
                     SEKYCYYHQWSEGELLLLDNRRTLHGREAFQGQRSLVNIQVIAEA"
     gene            complement(13880..15361)
                     /locus_tag="VGP27_12715"
     CDS             complement(13880..15361)
                     /locus_tag="VGP27_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7852195.1"
                     /translation="MLWESIWKAVVDQGISSHPAIVSDEAELTYGQLLSHVDALAHAL
                     RKIGVGPGHVVLTKLDNTPAFLVAILASAQLGAAFAPIDIGATDSEVEGIVALTGSNL
                     VVAGSAGQPRLADHFHNIVRVDAAGALTDVQSTGLAPPSVDATIGCLQFSSGTTGLSK
                     GVLIGHEALCYRTQYYMRLLGLGAADRTLCAVPLSHAHGSETLALPTLLAGATLYLKS
                     PKYAFPLYILEEIAQHRISFFSSVPAFYDAAVKLSLTQPPDLATLRLAACGSAPLARS
                     TAQEFHARFGVGIQQIYGLAELHAICMNRHDGDRMSYDSVGKPVEGVEWRIAGRPGQG
                     TAAEPEGELVVRSKAMFSGYLNNEEATRAKLKDGWLLTGDIVSVDRDGLFHIVGRKED
                     FIKVNGFKVYATEVEKAIIGLAWVKECAVLADKDAVGSERIVAHIVPADTAGAPEDME
                     ALLVKELRGVLSEYKLPKRCVVWPALPKSPMGKILKSKIVARS"
     gene            complement(15361..16563)
                     /locus_tag="VGP27_12720"
     CDS             complement(15361..16563)
                     /locus_tag="VGP27_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852196.1"
                     /translation="MKAYLLRNPCPLPPFQKPASRVRLRDSTVGSRLRAQLVEIGCEV
                     VDVDALDRSSMAPGSLAVQDDLVVSSSLLRLFVSAIPDRRRSYQCEIDTTRFSFTTLG
                     EAGAPPVFRAIPLHYRGDRGEAQAEPLRMIPSPVYEVSQGLPPRVDQITDMRAYVLDY
                     YGIQLEYWFDLLVASSLYCREYVADRLRSFHGVLPQRLMTRLTSWPRFRERSNSIGKG
                     TRIHPTAVVEGCVVGDDVEIGPFACLRSSVICDGTVLRERSTVTMSFVGERVFLAGCD
                     VLNSYIGCETAIFGPKLYNAVFGERGLLGGASGFGDFLIGNGPVVATIDGKKVPSNLW
                     LLASAVGDDCFVGTNMVFAPGQTIPDGTRVFDHGLVKFAPATSGGTYVVSGSDVIQVP
                     DGFMGGVR"
     gene            complement(16560..16796)
                     /locus_tag="VGP27_12725"
     CDS             complement(16560..16796)
                     /locus_tag="VGP27_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEV7852197.1"
                     /translation="MTDDEQKVIAAIQTLLGREVEITASTSLRDGLGLDSNALVELTV
                     LVHTMYRVDLGRRAAERKVIPETVGDVAALLATP"
     gene            complement(16837..17676)
                     /locus_tag="VGP27_12730"
     CDS             complement(16837..17676)
                     /locus_tag="VGP27_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7852198.1"
                     /translation="MYFAVETPFGPNHDEGDGLVKQAYKTLRVDVEDGIARVTFRRDK
                     DTNAFCRDMTLDLTDVCRKAAQDELSFEPRIAALVLTGGTGRSFSVGGDFNDVSVLEH
                     EHEVRPWLGEIIDLYIAILSVNVPVVAAMDRFAIGQGLQVALMSDWRIGTTECRLSMP
                     ELKNGVACPLGSVILEVLLGRSKMLELLLDCEFIDADAGRALCLLNEITAPSELEAAA
                     VRAAKRLATYPRTPYVTTKRIHNGRFIAALEEVREPSSQAHAASFKQHTGKAHFDKIL
                     KRA"
     gene            complement(17616..18980)
                     /locus_tag="VGP27_12735"
     CDS             complement(17616..18980)
                     /locus_tag="VGP27_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV7852199.1"
                     /translation="MSNGAPRCAVDEARLEELLCPYRGKIQRVAIAMHGIVGVLRLSS
                     LAGVYESKCVIPADTRKNAATAWLEITVDPTGLTFQTDPLGTFPLWYFEDDARVVITS
                     EVKSLTALDGVAVELDDYALREARHPADFSPFRGIQRVYPGAILRVSPTLELTEERRT
                     PLTYRPASMFATDAASGDALDAALTSSARAICGDPVAQATWGTFLSGGIDSSLATALT
                     RMCQPNVQTFTLGTDLGNEYAEAEELASHLSLSHTRVAADAGSAGAHFERAVFCNEMV
                     DGLTAETLAQIGVLAEAASKKVQRVVTGYGADLLFGSMLRHEQYMKATAVDDLQSLIE
                     RTCWTGEFAPFYAWSHGIEIHHLFWDPAVMNAAFRIPAESSFDGTEEKVLLRRVAAER
                     GHLDHRHVRRKKQAMTDGTQFNRLLSSALGLGDSYAYGQKSARCISQLKRLLDPTMTK
                     GMAS"
     gene            complement(19278..20504)
                     /locus_tag="VGP27_12740"
     CDS             complement(19278..20504)
                     /locus_tag="VGP27_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852200.1"
                     /translation="MTQTGQGAEAGGLQPPLQIRRHGLDPTPELSEVRASTGLRTVLN
                     VFGVPVHLVTRYNDVKAVLSDHGRFSNSGPAGFVMPGAPPPSADETADPRAGNLLGLD
                     PPEHQRLRRMLTGEFTARRMARLQPRITEIVKKQLDEMERRGAPIDLVSAFALPIPSL
                     AICELLGVPHDEREGFQRRSDRQLDLSLPFAERYELQRDSHDYMRSLVEEARVHPGDD
                     MLGMLVREHGDDLSDQELTAICGLLLLAGHETTSNMLGLGTLALLRHPAQIAAVRDDP
                     DAVGAAIEELLRYLSVLHHSIPRVTTADVEICGVTIPAGELVLASITAANRDPAFLGD
                     PDILDIHRDAASHVAFGHGVHHCIGAPLARMELRIALPALFRRFPHLALAEDFETVKY
                     RSFHLIYGLRSLAVRW"
BASE COUNT         3707 a         6493 c         6716 g         3652 t
ORIGIN      
        1 catcgagcgc ggcgactacc cgaaatgggg cgtctacatc caggtgatgc ccgaagcaga
       61 cgccgccacc taccggtttc accccttcga cctgaccaag gtgtggagcc agaaggacta
      121 cccgttgatc gaggtcggcg aatacgagct caaccgcaac ccggacaact acttcgccga
      181 tgtcgagcag gccgcgttca ctccggccaa cctcatcccg gggatctcgt tcagcccgga
      241 caagatgctg cagggccggt tgttctcata cggagatgca gcccgctacc gggtgggcgt
      301 caaccaccac cagatcccgg ttaactatcc acgtgccgcc aaggtggtga acgcctatca
      361 ccgcgacggg tcgatgcgcg tcgacggcaa ccagggtgga gtcgcgggtg ttgagccgaa
      421 caacttcggc cgctgggccg agcaacccgc ctacgccgat cccgctgagt cgatcgggtc
      481 caccgcggat cacttcgact tccgcgagga cgacgacaac tatttcgagc agcccggtga
      541 cctgttccgg ctcatgaccg gcgacgaaca acaaaggctc ttcgacaaca ccgcacgcgc
      601 catcaagggc gcccggcacc aaacggtcga aatgcacatc gcgaactgca cccaagccga
      661 cgccgcctac ggcgaaggcg tgcgcaaggc gtgtgaagcc ctcggggcgc tctaaccggc
      721 aaccgcgccc aactcaccgg aacccttgcc gtccagcgcc tttggggcgg caaggtccag
      781 cgctcgcccc actttgacca gtttggagtt cacatgacag accacttcgc tgaggaccca
      841 ggatgccagc tggatcccga actcgtggac ctggcatcgc gcatattcga gatggcgcgc
      901 aacggggacg ccgcaacctt ggccgtatac ctcgacgcgg gcgtaccggt gaacctcacc
      961 aacaccaacg gcgacaccct ggtgatgctg gcggcctacc acggacacga gtcgaccgtc
     1021 gccgcactga ccgcgcgtgg ggccgaggtt gatcgacaca acaaacgagg acagaccccg
     1081 ttggcaggcg cggtcttcaa gaacgacacc acgatcatcg aactgctcct acgagccgat
     1141 gctgacccgc tggcaggctc gccgtcggcg cttgaaaccg cacagttctt caaaagagag
     1201 gaacttgaac ggcagctgcg attacacatc gacggcgcgg ctgcgcaccg gtctagtggg
     1261 gagagcccgt tgcccctggc caagtaaatt acccaccacg ggtgcatggt catctctcac
     1321 ggttgccggc acttccagct tgcagagccc atgccactgc agtcaccgca cgtatcaatg
     1381 cgtctccaag aaggtgatca ctgcgaggac ccggcgatgg tcgtcgccgg tctcgggcaa
     1441 gttgagcttc gtcaggatgc tgcgcacgtg cttttcgatc gtgccttcgg tgatccaaag
     1501 acggtggccg ataccggcat tcgaccgacc ctcggccatc agggccagca cctcctgctc
     1561 gcgcgcagtg aggacagcta gcgggtcgtc gcgccgtcgc gcggacacca actcctgcac
     1621 cagtgcgggg tcgacgaccg acgcgccctt ggcgacgcgc tgcaacgtgt cgatgaaatc
     1681 cgcgacgtcg gtcacccgac tcttgagcaa atacccaatg ccgtggccgc cagccagcag
     1741 ctccatcgca tgctcgatat cgacgtgggc cgagagcacg acgacggcaa cgtgcggcaa
     1801 ttcctcccga ataactcggg ccgcatcgag cccttcggtg aagttggcag gcggcattcg
     1861 aatatcggtc accaccaaat cgggcctcgt ttcgcgaaca atggcgagga gttgtacgcc
     1921 gtcgccggcc tgcccgacca cgtcgaaacc agagcgttcc aacagactgg ccagaccctc
     1981 gcgcagcaag agatcatcct ccgccaaaac cacgcgcacc cgactccgcg gggccgtcgc
     2041 aatccgcgcc gggctagatc tgggcgtttc tccaccgttg acaccgagcg gcacgtccat
     2101 gtcgtcgacg gccagaccgt ggcgactttg gatgtgtcgc aacatgttcc caaggtcagc
     2161 cgccgttgcc ggccgatcgt ccggactgct cgccattgcg tgttcgattg cggcgctgac
     2221 atcctcgggt atttgaacca ggtccctcgc cgcggtgggc ccggcggcga gcctcaggaa
     2281 ctgggccact acctgctcgc cggtgcggcg ctcggaagcg gcgtgaccgg tgagggcaca
     2341 aaacagggtc gcgcccaagc tgtacaagtc ggaagcagga ctcgggctgt gtcccaacag
     2401 gacctcaggt gccgtgaacg ccggggatcc ggcgatgatg ccggtgccgg tttcgaagcc
     2461 gccggaaatc cgagcgatgc cgaagtcggc caactgaggc tcgccgtact cggtgagcag
     2521 aatgttggcc ggtttgatgt cacgatgcag cgtgcccagc cggtgcgccg tctcgacggc
     2581 tccggcgatc ttcactccga tgcgcagtgt ctcggcccac gagagtgggc cgtcggcgtg
     2641 gatgcgtgca cgcagggagt cacgcgggtg gtattgcatg acgatgaacg gccggcccga
     2701 cgtcgtggtg ccgacgtcga aaatactgac gatgttcgga tgcccgcaga gtttgcccat
     2761 cgcacgctgc tcgcgcagga accgctcgat gttgtcggaa tcgaggtccg tggtgaggac
     2821 cttcaccgcg acgacccgct ccaatgaacg ctgctggcaa cggtagaccg ctccgaagcc
     2881 gccggcaccg acctcagcgg cgtcgtcgag cccggccgcg gccagctccg cggcgatagc
     2941 cggcggtaga tcacaccggc cggtgggcgg ttcgcattcg gccatcagcc acctcggccc
     3001 cgatactcga tacctgcgat tgcacaccca tgcaataaca cgtcgaagcg atcagtcgag
     3061 cataactcct acaaggtgcc actgggcggg tcggtgagga tgcttcgaac aggatcctgg
     3121 ccagcaatga cgtccccgcg acgggttgac tcgagatcga cagggtgtca ccgagcgcct
     3181 caacgcgggc acgagatgcc ggtcggatag cgctgcggcc gcccctcccg actctggata
     3241 ttcgtactcg aagtcctccc gagtacgtag gcgatgccgc cgtggcgctt aaatagaacc
     3301 aagcagagat cgggcgagat tgcagtggca ggctactgcg ctcgtcgcac cacagggcaa
     3361 ccacgatttc ccctgaggag cgaaacatct gaccctgtgg ccgtcgtttc gccaggacat
     3421 cgcggaaggg acgacgatgg gtggggcaag cacatcggtc gccagcgacg gcctcagcgc
     3481 tgaccgacct tcgctgaccg gaccatccga tcacttggcg gttctgggcc ggatccgggg
     3541 tccgcgtgac ttgcagggat tgtcgcgcga tgagttgaca agactcacag acgcgatcag
     3601 agcattcctc atcgaaaagg tgtcggtgtc aggcgggcac ctcggtccta accttggtgt
     3661 ggtcgagttg accatggcgc ttcatcttgt attcgaatca ccgcgcgatg cgctgatttt
     3721 cgacaccggt catcaggcct acacgcataa gattctgacg ggccgccaag acgacttcga
     3781 ccacttgcga gcctccggcg gcctgtcagg gtatccgtcg cgcgcagaaa gcgagcacga
     3841 ctgggtcgag tcttcgcatg cgtcggcggc gctttcctac gcggacgggt tggcaaaggc
     3901 attcgaattg accggcagta aagatcgccg cgtggtcgcc atcgttggtg acggcgccct
     3961 caccggtgga atgtgttggg aggcgatgaa caacatcgct gccggcaaga gccgtcgtgt
     4021 cattgtcgta gtcaacgaca acggcaggtc ttatgcaccg acgatcggtg ggcttgccga
     4081 gcgtctgacc agcctgcgga cgcgtcgcag gtatgggcgg ctcctggcct tagtgaaaag
     4141 ggagttgacc gggttgccga cgctcggacg gtccacctat tcggtactgc acgcggtaaa
     4201 ggaaggattg aaggatgcgt tgagtccgca gacgttattc ggggatttgg gcatcaggta
     4261 tctcggcccg atcgacggac acgatatctt cgcgctcgag aaggctctgc ggatagcaac
     4321 ggacgtggat ggtccggtca tcgtccacac ggtcacgcgc aagggaatgg gataccgccc
     4381 agcggaggaa gatccggctg atcgcatgca tgcatgcgga ccgatcgacc ctcgaaccgg
     4441 caaacccaag tcaggcgccg atgttacgca atggaccgac gtctttgcca gtgagctgct
     4501 ccgatggggc agccgccgcg acgacgtcgt cgcgatcacc gccgcgatgg cggggccgac
     4561 cggtttggcc gcgttcggaa agcagtttcc caaccggatg ttcgatgtag ggatcgctga
     4621 acaacacgct gttacatcgg cagcggggct ggcactcggc ggcatgcatc cggtggtcgc
     4681 cgtttattct acgtttctca accgggcctt cgatcaggtg ttgatggatg tcgctgtgct
     4741 gaaattgccc gtgacattcg ttctcgaccg cgcgggcatt accgggtccg acggggccag
     4801 ccataacggc gtgtgggatt tgtcgctgct gggcatggtt cctggcatga gagtcgccgc
     4861 cccacgcgat gcggcttctc ttcgtgaaca gctcggcgag gcgctcggtg tcgacgatgg
     4921 cccgaccgcg atccggttcc ctaagggatc cgtgggagcc gataccaaag ccctggaacg
     4981 aatcgagggc gtggatatgc tttgccgccc gcaggatccg gatgttttgt tggtcgcggt
     5041 cgggccgctg gcgtcagtcg cgctgagggc ggcggcgaca ttggccaagc gtggcgtggc
     5101 cgtcgcggtg gtggatcctc ggtgggtcct gccggtgccc gagcaagtgg tcaagcttgc
     5161 cggcaacgtg cagctggtcg tgacactgga agagtcgggg ctacacggcg gggttgggtc
     5221 ggccgtggcg gcccgcatgc gcgaggcccg aatcgacaca cccgtgcgca gtgtcggtgt
     5281 gccgcagcga ttcctcactc acgcctcgcg cagcgagatc cacgccgacc taggtctaac
     5341 ggccgccgac ctcgccgcca acattagttt ctgggttcag cggccagctg acgttgatgt
     5401 gaaccggggt gaatgacgat gcgccacaga gcatttgcgg tagccgagcg accgatgccg
     5461 ccacattgca acagatcgcc gagctgacga cgtgttgcga ggaattgctc gactcgcgat
     5521 cgcggcgccg cggcggatga ttgcggtcgc actgctgatc atgatcggcg ccggcatctt
     5581 cggcatccct gtgacgaaga gcctgtccgc cggcggtttt caggatccaa ctagcgagtc
     5641 gtggcgcgcg gctcgactgc tctccgagag gttcggccag ggcgacatgt ggatggttat
     5701 cgcggtcacc tccgacgccg gcatcgccag tccagccgca cgcgccgtca gcgccgatct
     5761 cgtccggcat cttcaggcgt cgccccatgt tgccgatatc agctcggcgt ggaccgcgcc
     5821 gccgccggcc gcgccggcac tgatcagcaa ggacggcaag accgggctga tcatcgccgg
     5881 gatcaccggc ggcgagaatg gcgcccagaa acacgccaag cagctggccg atcagctggt
     5941 gtacgaccgg gacggggtca ccgtgcgcgc gagtggcgac gcggtgacgt acgtgcaggt
     6001 caacagccaa agcgaaaaag atctgctgcg tatggaattg atcgccatcc ctttgagctt
     6061 cgtggtgctg gtctgggtgt tcggcggcgt cttggcggca gcgctgccct tgacggtggg
     6121 cgggttcgca atcctcagtt cgatggcggt gctgcgcgca atctcttacg ccactgatgt
     6181 atcgatctat gcgctaaatc tcgctgccgc actgagtttg gcgttggcca tcgactacac
     6241 gctgctgatc atcagccggt tccgtgacga gcttgccgac ggcggaacac ggaacgcggc
     6301 gctaatccgc acgatggaga cggccgggcg gacggtgctg ttctcggcga tgacggtcgc
     6361 gctgtcaatg gtggcgatgg tgctgttccc gatgtacttt ctcaagtcct tcgcctacgc
     6421 cgggatcgcc gtggtgggat tcgcggccgt tgcggcggtc atggtgaccc cagcggcgat
     6481 cgtgttgctc ggggaccggc ttgactcgtt ggacgtgcgc cgactgggcc gaaaggtgct
     6541 gagacggccc gagccggtgc aacgtcctgt cgagcagatg ttcttttacc gctcaaccaa
     6601 gttcgtgatg cgtcacgcca tcgtggtggg cctagccgtc accgcggtgc tgttgatcct
     6661 cggcgcacca ttccttggca tcaagtgggg cttgccggac gaccgcgtac tgcccgactc
     6721 cgcgtcggcg cgccaggtcg gcgaccagat tcgcagcgct gctttcgccg tcaacttcgc
     6781 gaacaacacg accgtcgtga tcccggactt gaccggggtc acagccgacg aactcggccg
     6841 ctatgcggca cagctatcgc aggtcgccga tgtatcgtcg gtctctgcgc cgggcggcac
     6901 cttcgttgac ggccggctgg ctcggcctga gtcggcggcg gcgatcaagg acggcagcgc
     6961 gtttctcacc gttaacagca ccgcgccact gtactcagaa gcttcagaga tccagttgga
     7021 ccggttgcac gccgtcgcgg gccccggcgg ccaacacgtc cagatgaccg gaatcgcgca
     7081 gatcaaccgc gacagtggcg aagcgatcac ctcacggctg cccacagtgg tggcggtgat
     7141 cgccgctatc acgttcgtgc ttctcttctt gctgaccggc agtgtggtgc tgccattgaa
     7201 ggcgttgctg ctcaacatct tatcgctctc cgccaccttc ggtgctctcg tgtggatctt
     7261 ccaggaaggt catcttgacg cgttcggcac cacggctaca ggcacgctgc tggccagcgt
     7321 ccccgtgctg ttgttctgca tcgcatttgg actgtcgatg gactacgagg tgttcctggt
     7381 tgcgcgtatc cgcgagtatt ggttgacgtc cgcatgcccc ggggctgaca acgatgaaag
     7441 cgtcgcgctg ggcctggcgc gcaccggccg ggtggtcaca gcggccgcgt cggtaatggt
     7501 catttcgttc gcggcattga tcgccgcgca ggtgtcgttc atgcgaatgt tcggcgtcgg
     7561 cctgactctg gcagtgcttg tggacgcgac gctggtgcgg atattgcttg tcccggcgtt
     7621 catgcacgtg ctcggccgtg ccaattggtg ggcgcccaag ccgatggccc ggcttcactc
     7681 gcggttgggc atcagcgagg cacgcgcacc tgaacccacc gaggtaacgc cgaagaagac
     7741 cggtcagcct gtcgacatcg ggtcctaatc cgtcgagcac gacaacgtcg taggaggtgc
     7801 ccgcgatgac gaaccgctac ctcgaagcgg cccttgcgcc tatcaatgag gaatacacac
     7861 tgaccgatct ggaggtaacc gggacgatcc cgacgtacct ggacgggcga tacctgcgca
     7921 acggtgcgaa cccgatcagt gaggtggatc cagagctgta tcactggttc accggcgacg
     7981 gcatggtgca tggcatccgc attcgcgatg gccgggccga gtggtacggc aatcgctggg
     8041 tgcgcgggcc gttggcgaag tcaaagctgg gcgaggcgcc gccgggcggc catttcggcc
     8101 tctatggcat tggcgccaac accaacgtga tcgggcatgc cggcaaaacc atcgcgctga
     8161 tggagggcgg gttggcgaac tacgagctta ccgacgatct ggacaccgtc ggcgtctgtg
     8221 acttcgacgg gacgctgacc ggcgggtata cggcccatcc caagcgcgac ccagaaaccg
     8281 gtgagctaca tgcggtttcg tacagatgga accgcggcaa caccgtccag tactcggtga
     8341 tcggcatcga cggccgtgcg cgtcgcactg tggacattga ggtcacgggc agcccgatga
     8401 tgcacgattt ctcgttgacc aagcgtcacg tggtgttcta cgacctgccg gtcactttcg
     8461 acgtgggccg ggccgcggcg atggcagtgc cgcgagggct gcgcctgccc gcgcgactcg
     8521 tgttgtccgc gttgatcggc cggatccgca tccccgatcc cattgcggcc cggatgccct
     8581 gcggcacgga ctccgaccgg cgattaccgt actcgtggaa cccggactat cctgcgcggg
     8641 tcggcgtgat gcctcgcgac ggaagcaatg gcgatgtccg ctggttcgac gtggagccct
     8701 gctacgtcta ccacccgatg aatgcctacg acgacggcga caccgacatc gtgctcgacg
     8761 tcgttcggca ccccaagatg ttcgacaccg accacagcgg tcccaacgaa ggtccgccga
     8821 cgctggaccg ctggactgtc gatctcgtcg gcggaaaggt gcgtgaatcg cgggtcgacg
     8881 atcgcggaca ggagtttcct cgcgtcgatg agcgtgtggt tggaaagccg caccggtacg
     8941 gatatgcgcc gacggtcggc gaggacggca cggcaggcga ttctttgatc aagcacgacc
     9001 ttgtcggcga gaacacgcag agccgctcct tcagcgccga taaaatgttg ggcgagtttg
     9061 tctttcatcc ttcgtgcccg gacgcagagg aggacgatgg cgtgctcatg ggctacctct
     9121 acgaccggcc gaccgatcgc agcgaactgg cggttctcga cgcgcggacg ctacaggacg
     9181 tggccagcgt caagctgccg caccgggtgc ccgctggatt tcacggcaac tggataccga
     9241 cgcaagcagg cttttcggac gtgacaaccg ccggttagag ggactgctgc acacgggaag
     9301 cgacgcgctg tgctgtcgcc gccgcgcgct cgactcggcc gccgacggcg gtccgagagc
     9361 aggtcctacc tgctggcgcg tgctcagatc cacgcgaacc agggcaggta ccgcgagcgg
     9421 acggtaccct cctggattcg cttgtccagc ttggccttcc atcgctcccc gtaggcggcg
     9481 gtgagaaagc ccccgggcac aatcaggcgc cagatcgagt tgagcgtcat ccagtagggc
     9541 acgatgacgt ccggctcaac cttctgcatg aaatcgagct tgtcggggat ctggctccag
     9601 tggatgccgg ggtagcggtg atgcacactg tggtagccct ggttgaagat gaaaaaattc
     9661 agccagttgc ccacggtgtt catcgatttg ctcgggtctt cctcgaagcc acgcgccggg
     9721 gcgtgggtca gccaggcgaa gtagccggag ttcacctggg tcacgatgaa cgggatcgcg
     9781 tagaagagcg tgaaccgggt ggtgtcggtg acgtaccaag tagcggccat cagcgccacg
     9841 acaagcgtgt agtcgagcat gaattggtag cgccgcttgc gccgaccggg cgatgcattg
     9901 gcggcgaaag tcgttcgcac ggtgtggttc ttgacgatga tgccgtagct gatccagtac
     9961 cagacggcgc gcagcccctg ctcgcgcccc tcggtggagg tgacgtcgcc cggcccgtcg
    10021 ttgtagcggt ggtggttgag gacatgtacc tcgcgcattt cggctgctgt cagcgtggcg
    10081 ggcatgcagc acagtacgtc gacgacccgg ttcatccgcg gcgacacgaa aagcggccga
    10141 tgggtgtgca tgtgcagcac gccgattgtg atcgagaggt tcgccagcgc cagcaagacg
    10201 atcagcggca ccgcgaggta ccagttcgtg gcggccggca cgagcagagc gaaggccatc
    10261 agcccgaacc agcccgcgaa atgcgcaaga ggcccggcat tcgccgggtg ctcaagcttg
    10321 agcacccggc ggctgaaggg cattcgttcg gacggcgcga cggatgccgt ggcgctgggg
    10381 agggtcaacc ttctggtcat gggcgacctc cttgggtagc ggcgccgagg gcggtgagct
    10441 gcaattgcgt tgcgagggcc tgcatggcgt cggcgtcgcg gggtcgcagc cgttccaggg
    10501 cgccgaaggt tagatggtcg gcaatcatca tgcgacgcac ctgggactcg ggcagttcgc
    10561 cggtcagcgg ttcttccggc agggcgtcga ctagggcgaa ggtggcgccg acgataagcg
    10621 ggcccattga gggcgacatc gagcgcgggt tacccagacg taggacgtcg gcagccgggc
    10681 ccgacccggt cgcgacgcca gcctccgcta acgggttcgg cgatgcatcg agcagccggt
    10741 ggatcgcccg gtcggcggaa cgccgcacct gctccagcat cctggcccag tgaacgggtg
    10801 agtgggcggc cagcattgcc gcggccagct gcagccgtcg ccggtagggc gccggcgcgc
    10861 cgacggatgc gcacaagaac gctgtcgtcg tgatggtcat ataggccatc gccacgcggg
    10921 cgccgcgact gggggtgcat gggccgagcc gggtgagcag cgcgaagccg cccagcgcgg
    10981 gggccagcag gcgcagcatt accgcccggg tcagttcctc gcggtcgccg gcgtcataca
    11041 gggccagttc gccgtcgcgc aagtcgttgg ccaactgagc cgtgacgccg atgcatccgg
    11101 ccagctcgct caaatcggtc tgggcacaag cgtcttcggc gaccagtgag catacgtaca
    11161 gcaccacccg cccgagcacg ccctcgccgt aggcggtccg cggcagtggg gagtcgaggt
    11221 tgtgggccat cacgtggccg acatcgacta gcatccggcc gactcgttta cgtccctcga
    11281 cgggcagttc cgacagcagc gcccgcacgt cgcggattcg ctcggccagc accaggtcga
    11341 ccgcttcgct gtcgcggacg gcgatcgcct gcagcggcgg tggcagcgtg tcggtgtcgc
    11401 cgttcaggta gtccacggcg gtcacgacgg cgccggccgc cagcgggcgc tcactcaagt
    11461 cttcgtaggc gtcgagaacc cggcaggcca acagggcggc ggtggcctcg gtccgtaggt
    11521 ccgtgggaag gaaaccggct gcgatgccga ggttccgggc cgccgggatt agcgccagcc
    11581 tggccagctc acccggtgat cgggccgccc gcatcgcgtc caagtccggt gcgcggtgca
    11641 gaaacctcag cccgtcggcc agcgtgccga gtcgcccccc gctcatcgga acaccacccg
    11701 caccgcgagt tcgcgcaagt gctcccgcgc gggggaaggc gggaacacgt ccatcgtccg
    11761 gcgggccctc tcggcggcgc ggacggcgac ggcacgggcc gctcgggtgg ccccgctgtg
    11821 caccaggtcg gcctgcaggt cggcactcga agtcagcccg gagagccaca tgtcatggga
    11881 atcgggccgc gtcgccagcc attcgatcac cggccaggtc ggcacgcggc ggtcgaggtc
    11941 gccaccggcc tccttgccca acgaggggcc gccctcgacg tcgcggacgt catccatgat
    12001 ctgaaaggca agggcgagtt ggtcggcgta ttgcatcagg acgcacagcc gatcgtggtc
    12061 gacggcgccc gccgtgccgc cgtacgagca ggccagccgg aacagggtgc cggttttcgc
    12121 gccggccacg actcggtaat gccggcgcag ggcggcgtcg tcgacggcag gtggcagggt
    12181 ctcgacgagc tggccgaagg acagctccga gatccggtcg agataggtga cgccgggcgc
    12241 ctcaccgagc cgcgcgaaca ccgccgggtt gtcagccagc actcgcgcga gtacctgtaa
    12301 cgccttgccg gccagatgaa atcccgcccg ggcggcggtg cggacgccga acgcggccgg
    12361 taccgacggc gcatcgcgcc gcaagagcga tccgtcgcag atgtcgtcgt gaacgagcga
    12421 cgcctcgtgg aacatctcga tcgcgcacgc cacatcgacg gcatcgcgca gcggagccgc
    12481 ggcagccgag ggcagcagac gggcacaggc cagcaccatt gccgcccgga cccgtttacc
    12541 ggggacggcc aggatgtgac ggtagatgac atcgaaaccg ccatcgctga ccaggatttc
    12601 gtgcaaccga tcggcgacca gggtcaattg atcggatgga ccggtcagcg aaggtcggcc
    12661 aacactgcgg ccgacggacg cgaccgatgc gctttgctca cccatcatcg tcccttccgc
    12721 gatgtcctgg cgaaacgacg gccaccgggt cagatgtttc gctccacaga ggagatcgtt
    12781 gctgccgtgt ggtgcgacga gcgcagtagc ctgccactgc aatatcaccg atttcggcgc
    12841 ggtactactc aaggccgatc ttgagagacc ggtcgagttc ggcgggcgcg ctgatgttga
    12901 ctgcgcggcc gcagggttct tgctgctgtc gttgctctca ccgttgcagc tgccgactgt
    12961 cgtgatacaa atacgcgcga gcacagatgt gaccgatgac tcctagaaca ccgccagcca
    13021 gcagcgtgac ccacgacgaa cttacggccg tgacgtctag gcctccgcga tgacctggat
    13081 attcacgagc gaccgctgtc cctgaaaggc ctcgcgtccg tggagcgttc gacgattgtc
    13141 tagaagcaag agttcgccct cgctccactg gtggtagtaa cagtacttct cgctgatgag
    13201 cgcggcggat agtgtctcga tgatcgtctg gaacagctct tcttcgacgc ccgggatgcg
    13261 gatcagccaa ctcggcttct cacccggtcc gtaggggaat atgacattga ggtagctcac
    13321 tccgccgaca tcgatgaagc cggggatcgg gagccaggca tctgacggct tcgtgtagta
    13381 gttgtcgacc ggcttgccct cgatgccttt ctcgcggatg atgtcgagca tagcccccgg
    13441 cacttccttt accgcgcttg cgatgtcgga gatgaaggtg cgaccgccgc cggtgacgtt
    13501 ctggtattcg acgcaacaaa tgccgacgaa tgcgatgtcg ttgcccatga aggccccgtc
    13561 tcgatgaagc ggcatgaacc cctcgccgag aaggacctcc tcttttttcg aaccgtctag
    13621 cttgaggatg ccgttctgtc gtcgatcgtt ctggaccagg cgcccgaact tgcgaaggtg
    13681 tccggccaca tcgctcccgc tgagcgaatg gtgtttgaac accagccact gataggtgtc
    13741 gatgagcgac cgaagctctt cgaccgagaa atcaaggaac gagcgatcgg cgatcgtcgc
    13801 gcctcggcct gactgagtat gtggttgata tggtttgctg gacaacatga ttggttcacc
    13861 gttctgcatg tgttctgaat cacgaccgcg cgacgatctt cgacttcagg atctttccca
    13921 tcgggctttt gggaagggcg ggccacacga cgcagcgctt cggcagcttg tactccgaga
    13981 ggacgccgcg cagctccttg acgagcagtg cttccatgtc ttcgggagcg ccggcggtgt
    14041 cggcaggaac gatgtgcgcg acgattcgct ccgatccgac cgcgtcctta tccgccagca
    14101 cggcacactc cttgacccag gcgaggccga tgattgcttt ctccacctcg gtggcatata
    14161 ccttgaaccc gttcaccttg atgaagtcct ctttgcggcc cacgatgtgg aacaggccgt
    14221 cccggtcgac tgaaacgatg tccccggtga gcagccagcc gtctttgagc ttcgcgcgcg
    14281 tcgcttcctc gttgttgaga tagcccgaga acatagcctt ggaccgaacc acaagctccc
    14341 cttcgggttc cgctgctgtg ccctgtcctg ggcgcccggc gattcgccac tcgactccct
    14401 cgacaggttt tccaaccgag tcgtaggaca tccggtcgcc gtcgtggcga ttcatgcaga
    14461 tcgcatgcag ctccgcgagc ccgtagatct gttggatacc cacgccgaag cgggcgtgaa
    14521 attcctgcgc cgtggacctc gcgagcggcg cgctcccgca cgccgccagc cgcagcgttg
    14581 ccagatccgg tggctgggtg agagacagct tcaccgcggc gtcgtaaaac gcgggaaccg
    14641 agctgaagaa cgagatgcgg tgctgcgcga tctcctcgag gatgtagagc gggaacgcgt
    14701 acttcggcga cttcaagtac agcgtcgcgc ccgccagcag cgtgggcagc gcgagggtct
    14761 ccgagccgtg ggcgtgactc agtggcacgg cgcaaagcgt ccgatctgcc gcaccgagcc
    14821 cgagcagccg catgtagtac tgcgtccggt agcagagcgc ctcgtgtccg atgagcacgc
    14881 ctttcgagag accggtagtg cccgacgaaa actgcagaca accgatggtc gcgtcgaccg
    14941 agggtggcgc gagcccggtg ctctgcacgt ccgtcagagc tccggcagca tccacccgaa
    15001 cgatgttgtg gaagtgatcc gcgagacggg gctggccggc gctgcccgcg acgacgagat
    15061 tgcttccggt cagtgcgacg atgccctcga cctcggagtc tgtcgccccg atatctatcg
    15121 gcgcgaatgc cgccccaagc tgcgcgctcg cgaggatcgc gacgagaaac gcgggagtgt
    15181 tgtccagttt cgtcagaacg acatggccag ggcccacccc gatcttccgc aaggcatgcg
    15241 ctaacgcgtc gacatgcgaa aggagctgcc cgtatgtcag ctcggcctcg tctgagacga
    15301 tcgccgggtg agatgaaatc ccttggtcga caacggcctt ccagatcgac tcccacagca
    15361 tcaacggaca cctcccatga agccgtcggg cacctggatg acgtcggatc cggagacgac
    15421 gtacgtgccg cccgatgtgg ccggggcgaa cttgacgagg ccgtgatcga atacccttgt
    15481 cccgtctgga atcgtctgac ccggtgcgaa aaccatgttg gtgcccacaa agcagtcgtc
    15541 gccgaccgcg ctggcaagta accagaggtt ggaggggacc tttttaccgt cgatggtggc
    15601 aacgacgggt ccgttcccga tgaggaagtc gccgaagccg ctcgcaccgc caagcagccc
    15661 tcgctctccg aaaacggcgt tgtagagctt tggtccgaag atcgccgtct cgcaaccgat
    15721 gtaactgttg aggacatcgc agcccgccag gaacacgcgc tctccgacga acgacatcgt
    15781 gaccgtcgac cgctcgcgga gcactgtgcc gtcacagatg acggaggagc gaaggcaagc
    15841 gaaaggtccg atttctacgt cgtccccgac tacgcagcct tcgacaactg cggtcgggtg
    15901 aatgcgcgtt cctttcccga tcgagttgga tcgttccctg aaccgcggcc acgacgtcaa
    15961 gcgcgtcatg agccgctgcg ggaggacacc atgaaacgac cgcagtcgat cggcgacgta
    16021 ctcgcggcag tagaggcttg acgcgaccag cagatcgaac cagtactcga gttggatccc
    16081 gtagtagtcg aggacatagg cgcgcatgtc cgtgatctga tccaccctcg gaggcagccc
    16141 ctgcgatacc tcgtaaacgg gggacgggat catccggagc ggctcggcct gcgcttcgcc
    16201 gcggtcgccg cggtagtgca ggggtatcgc gcgaaagacc ggtggcgcac ccgcctcgcc
    16261 cagggtggtg aagctaaagc gcgttgtgtc gatctcgcac tgatagctgc gtcgacggtc
    16321 aggaatcgcc gagacgaata agcggagcag gctgctggac acgacgaggt cgtcctgtac
    16381 cgcgagtgag ccaggagcca tagaacttcg gtcgagggca tcgacgtcga cgacctcgca
    16441 accgatctcg acgagctgcg cccggagcct cgacccgacc gtcgaatcgc gcagtcgcac
    16501 gcgcgatgcc ggcttctgga acggcggcag cggacagggg ttgcggagga gatacgcctt
    16561 catggcgtgg ccaacaacgc ggcgacatcg ccgacggtct cggggatgac tttccgctcg
    16621 gcggcccttc ggccaaggtc gacgcggtac atcgtgtgga cgagcacggt gagctcgacc
    16681 agggcattcg agtccaggcc gagaccatca cgaagcgacg tcgatgcggt gatttctacc
    16741 tcccgcccga gcagcgtctg tatcgcggcg atcaccttct gctcgtcgtc ggtcatcgca
    16801 tctccttcgc tgtccgcgtg cccgcgctcg ggctcttcag gcgcgcttga ggatcttgtc
    16861 gaaatgcgcc tttcccgtgt gctgtttgaa cgaggccgca tgagcctgcg agctcggctc
    16921 gcgcacctct tccagtgccg cgatgaagcg gccgttatga atgcgcttcg tcgtgacgta
    16981 cggcgtcctc ggatacgtcg caagtctctt agcggcgcga actgccgccg cctccagctc
    17041 gctcggggct gtgatctcgt tgaggaggca cagcgcccgg cccgcgtctg cgtcgatgaa
    17101 ttcgcagtcg agcagcagtt cgagcatctt ggaccggccc agaagaacct ccaggatcac
    17161 cgagccgagt ggacaggcga ccccgttctt gagctcgggc atcgagaggc ggcactcggt
    17221 cgttccgatc cgccagtcgg acatcagcgc tacctggagc ccctggccga tggcgaagcg
    17281 atccatcgcg gcgacgacgg gaacgttgac gctcaagatg gcgatgtaga ggtcgatgat
    17341 ttcgcccaac caggggcgaa cctcatgctc gtgctccaac acggaaacgt cgttgaagtc
    17401 tccgccgacg ctaaatgacc gtcccgtgcc gccggtcagg acgagcgccg ctatccgagg
    17461 ctcgaacgag agctcatcct gggccgcctt ccggcagaca tccgtgagat ccagggtcat
    17521 gtcacgacag aatgcgttcg tgtctttgtc tcgccggaag gtgacgcgag ctatgccatc
    17581 ttcgacgtcg accctcaggg ttttatacgc ttgcttcacg aggccatccc cttcgtcatg
    17641 gttgggtcca aaaggcgttt caactgcgaa atacatcgag cactcttctg tccgtacgcg
    17701 tagctgtcac cgaggccgag ggccgaggag agcagccgat tgaactgcgt gccgtcggtc
    17761 atcgcctgct tctttcgacg tacatgccgg tggtccaggt gtccgcgctc cgctgccacc
    17821 cttctgagca gtaccttctc ctcggtgccg tcgaaactgg attctgctgg aatgcggaac
    17881 gcggcgttca tcaccgctgg atcccagaag agatggtgga tctcgatgcc gtgcgaccag
    17941 gcgtagaacg gcgcgaattc ccctgtccag caggtccgtt cgatcagcga ctgcaggtcg
    18001 tccaccgcgg tggccttcat gtactgctcg tggcggagca tcgatccgaa gagaaggtcg
    18061 gcgccgtatc ccgtcacgac gcgctggacc ttcttggagg ccgcttcggc gaggacgccg
    18121 atctgggcga gtgtctcggc ggtgagcccg tccaccattt cgttacagaa gacggcgcgc
    18181 tcgaagtggg cgccagcgga ccccgcgtcg gcggctaccc gtgtgtgact cagcgataga
    18241 tgtgacgcga gttcttcggc ttcggcgtat tcgttcccga gatccgttcc gagcgtgaac
    18301 gtctggacgt ttggctggca catgcgggtg agcgcagtcg caagcgacga gtcgatccca
    18361 cccgagagga acgtgcccca ggtggcctgg gcgacaggat caccgcagat cgcgcgagcg
    18421 gaagaggtca gcgcggcgtc gagcgcatct ccgctagccg catcagtcgc gaacatcgag
    18481 gctggtcggt aggtgagcgg cgtacgtcgc tcctcggtca gctccagcgt cggcgaaacg
    18541 cgaagaattg caccaggata cacgcgctgg atgccccgga aggggctgaa gtctgcagga
    18601 tggcgagcct cgcgtagagc gtagtcgtcg agttcgaccg caacgccatc cagtgcggtc
    18661 aggctcttca cttcgctcgt gatcacgacc cgtgcgtcat cctcgaaata ccagagcggg
    18721 aaggtgccga ggggatcggt ttggaatgtg aggccggtcg gatccacggt gatctcgagc
    18781 cacgccgtcg cggcattctt gcgcgtgtcg gcggggatca cgcatttcga ctcgtacaca
    18841 ccggcgagcg acgacagccg aagaacgccg acgatcccgt gcatggcgat cgcgacgcgc
    18901 tggatcttcc cgcgataagg acagagcagc tcttcgagac gcgcttcgtc gactgcgcac
    18961 cgcggcgcgc cattggacat cactgtcgcg ggtcgtttga ggagcacaca cagaactggc
    19021 attagggctt ctgttccgag gcataatcat ggaccacttt ttcgcgacca cactttcggt
    19081 ttgcgatacc acgcgaaaca gggtcccgct tgcacccctc gtagccggcg ggatccaggt
    19141 ccacgtcgtt gtgaacgcgt cgggttgggt tcacgaggta atcgtcgcta gcgcgcggcc
    19201 cgacgaacgc agtagtgcgc cactgcaata tcgggtggta tcggcgcggc actacctagg
    19261 gcggctgcgg cgaacggtca ccagcggacg gctagcgatc gcagcccgta gatgagatgg
    19321 aacgagcggt atttcaccgt ctcgaaatcc tcagccagcg ccaaatgcgg gaaccggcga
    19381 aatagcgcgg ggagcgcaat ccgcaactcc atgcgcgcca acggcgcgcc gatgcagtgg
    19441 tgaaccccgt gtccaaacgc gacatggctt gccgcgtcgc gatgaatgtc gaggatgtcc
    19501 gggtcgccga ggaaggccgg gtcgcggttg gcggctgtga ttgaggctaa gaccaattca
    19561 cccgcgggga tggtcacgcc acagatctcg acgtccgcag tcgttactcg cggaatggag
    19621 tggtgcagca ccgacaggta gcgcagcagt tcctcaattg ccgcaccgac cgcgtccgga
    19681 tcgtctcgca ctgcggcaat ctgagcggga tggcggagca gtgcaagcgt gccgagaccc
    19741 aacatgttgg acgtcgtctc gtgtccagcc agcaggagca atccgcagat cgccgtcaac
    19801 tcctgatcgc tcaggtcgtc cccatgttcg cgcaccaaca tgccgagcat gtcgtcgccc
    19861 ggatggacgc gggcttcttc caccaacgac cgcatgtagt cgtgactgtc gcgctgcagc
    19921 tcgtaccgtt ctgcaaacgg cagggaaagg tcgagttggc gatcggagcg gcgctgaaaa
    19981 ccctcccgct cgtcgtgggg caccccgagc aattcacata tcgccagcga cgggatcggc
    20041 agggcgaatg ccgacaccaa atcgattggc gcgccccgcc tttccatttc gtcgagctgc
    20101 tttttcacga tctcggtgat cctcggctga agccgggcca tccgccgggc ggtgaactct
    20161 ccggtgagca tccggcgcag ccgctgatgc tcgggcgggt caagaccgag caggtttccg
    20221 gcccgtgggt cggcggtctc atccgctgaa ggcggtggcg cgccgggcat cacgaaaccc
    20281 gcggggccgg aattggagaa ccggccgtga tccgacagca ccgccttgac gtcgttgtag
    20341 cgggtgacca gatggaccgg cacgccgaac acgttgagga ccgtgcgcag cccagtggat
    20401 gccctgacct cgcttagctc cggtgtcggg tcgagcccgt gtcgccgaat ctgcaacggg
    20461 ggctgcagcc cgccggcctc ggcaccctgg ccggtctgcg tcatgggatg gacgctacgc
    20521 gcgctgccgg ttagtcccgt ttgagtgtga cctcttccag gtcgaggc
//
LOCUS       DASYLX010000524         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1999879,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000524 DASYLX010000000
VERSION     DASYLX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999879"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VGP27_12745"
     CDS             <1..151
                     /locus_tag="VGP27_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7852201.1"
                     /translation="DRAELDTWVEKLNKLGIEHGGIVDAHYGSGLSFRDPDGIALEFF
                     APPAP"
     gene            390..737
                     /locus_tag="VGP27_12750"
     CDS             390..737
                     /locus_tag="VGP27_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852202.1"
                     /translation="MKNIKTFAGTFAAGAAIAAASLLTVAAPASAAATHAAGQQAGIA
                     KTAQQRQAAGQRQAGVPKRQGSPLSAEEQREQQLQRAKAQKQAGQQQAQQPAQSTDPG
                     QFLPDPPVVTPEG"
     gene            complement(2228..2665)
                     /locus_tag="VGP27_12755"
     CDS             complement(2228..2665)
                     /locus_tag="VGP27_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852203.1"
                     /translation="MIAPDNSPEIFSAPLILPWTAGPCVTTTQPIVVSVTEFRVHHRR
                     DLPTVAVKGLRMRLGWYAMSGALGLWLWSLPVAMRAGSISVWDNDDSLERFINLPHHV
                     GIMSRYRSRGTVRSDKWSMERFEPDAVLDRAREWIVRPTICAR"
BASE COUNT          568 a          897 c          898 g          489 t
ORIGIN      
        1 ggaccgcgcg gagttggaca cgtgggtcga gaagttgaac aagctcggta tcgagcacgg
       61 cggcatcgtc gacgcccatt acggcagcgg gctgagcttc cgcgaccccg acggcatcgc
      121 gctcgagttc ttcgcaccgc ccgcgccata gccgaaggcc gatgacggcg gcagcggcgt
      181 gccacgcgtg tgacactgaa ccgctcgcag acgcccgagg cgttggtgcg tgcgggatag
      241 cgctgcgcac aatgcgcaaa acgcctggat caactctttt gcgacttgcg cgcacaagca
      301 cgacacccac aaccacggtt cgtaatctca aaggcacaaa cagatttatc gagaagagct
      361 cgaagaacgc gaaccgtgag gaacgaatca tgaagaacat caagactttt gccggcacct
      421 ttgcagccgg cgccgcgatc gccgccgcgt ccctgttgac ggtcgccgcg ccggcctcgg
      481 ccgccgccac ccatgcggcc ggacagcagg cgggcatcgc caagaccgct cagcagcggc
      541 aggcagcggg ccagcggcag gccggcgttc cgaagcgaca ggggtcgcca ttgtcagccg
      601 aagagcagcg ggagcaacag ttgcagcgcg cgaaggccca gaagcaagcg gggcagcagc
      661 aagcgcaaca gcccgcgcag agcaccgatc ccgggcagtt cctgcccgac ccgcccgtcg
      721 tcacccccga ggggtgacct cgggcaagtg cacgcagccc gctctaagac agtcagagat
      781 tttctctggt cgggctgaca gggcttgaac ctctcgcaga ccgatgatcc gcgtgcaccc
      841 gatgtgacgc cagctcgacc atgaccgcag agcagcacgt tggcgtttgc agccacgctg
      901 acggcgcgct acagcggcac gccgccggat aagtaaccgc cttcggcgca cgacgcgtca
      961 tcgcgagcct cggcggctac cttgtcgcga aacaagcaaa cttctccgcg tttcacaaaa
     1021 gtgatcgcgt gtgctggtgg cgcgaatatt aatgaataca tgatgcgggc ttaaagtcac
     1081 gtcctcggtg ccatttttat tttcgaacgc aacatttacg gatcccgaaa tgaaggcggt
     1141 atttctgaac cgacgactcg cccgtatctg tagaggcggg acgcctcgag ctgcgacgat
     1201 cgccgggcaa ttcgccctgc caagacggtg gagaacagcg tgatgtcgtc gagaaaggga
     1261 ccttcgtgcg tgggcaattt agagggaatt gtcatcgtgc cgcggaagcg accgaatggc
     1321 ggtaccttgt gctatgactt ctctggggga aatgtgcgct aatcgccgtg cgatcatcgc
     1381 ggcgctgccc gggctaattc tgctaccggg tgcggtcgcg gcctgttcgt cgtcagaatc
     1441 atcgcccgct gccccctctc ccgctgccgc ggacgccggt gatcaatccg caagatcaca
     1501 ggccaccgct ttgaacgcgg tccaggtaca gcaatcgcag gtgccggtcg gaacggctgt
     1561 cgttgtctcc gggtacgtgg tggcacaacc ggtgtcgggc cagttcgtcg cttattctgc
     1621 agcatgcacc catcgaggag tcacggtcag tgccgggaac ggaacgacgc tgcactgtcc
     1681 tgcccatggc agtgaattcg atggcgcaac tggggcggtg ctgaacggcc cggcaagcag
     1741 cccgttggca ccggttacgg tcaacgccag cagcggtgtt ctgacgatcg gttagttgcg
     1801 gcgatccggc cgcgatatcc cggaccctgg gaaaagatca ccatgaaggc cgcatgagtc
     1861 ccggtgcgaa atcacgaatt tcgtggagtg atcgcaaaca acccggcacc ggctaaccct
     1921 gcgccgcctg ccatggtcag tcctagccac cttttggctg gcggcatgtg gggccaggcg
     1981 ccggcgattc ctgcgacgtc gctactgtct gccagtaatc cggcaatcag cccgggtcgg
     2041 acggactcgc cgttacggat cgcggtcacc agtccgagtc cgattccgag ttcgcggccc
     2101 cccatgccgc gcaaaaacgc tgtcgcgtcg ggcgtggcgc cgccgtcgcc ggcaagtaac
     2161 ctgccgactg tcttaggcgc cgccagcgca gctaggccaa aaatgagacg tgcaaaggtg
     2221 tacagcgtta tcgggcgcat atcgtcggcc taacgatcca ctcgcgtgcc cggtcgagca
     2281 cggcatcggg ttcaaaacgt tccatcgacc acttgtcgga acgaaccgtg ccccgggagc
     2341 ggtagcgact catgatgccg acgtggtgag gcaggttgat gaaccgctcg aggctgtcgt
     2401 cgttgtccca cactgatatc gaaccagcac gcatagccac tggcagcgac cacagccaga
     2461 gtccgagggc gccgctcatg gcataccagc ccagccgcat ccgcagcccc ttgactgcca
     2521 cggtcggcag gtcgcggcgg tggtggacgc gaaattcggt gacgctgacg acgattggtt
     2581 gggtagtggt cacgcaggga ccggccgtcc acggcaggat caaaggtgcc gagaagattt
     2641 cgggagagtt gtcgggtgct atcacggctc tagcctcagc ctagtattgg tctatgtcac
     2701 ggtccaatag caccttttct gaacggacca attcatccga caccggcttt ccgatggaga
     2761 tcctgctcca agtggaccgg tcaaggcgcg gcgtgcgcca acagctcacc caacagttgc
     2821 gtgcaatgat ccgagacggc cggctggcgg cg
//
LOCUS       DASYLX010000525         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_574012,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000525 DASYLX010000000
VERSION     DASYLX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574012"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VGP27_12760"
     CDS             complement(<1..888)
                     /locus_tag="VGP27_12760"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEV7852204.1"
                     /translation="MTRAHVSIPRTAVVPAAGLGTRFLPATKTVPKELLPVVDTPGIE
                     LVAAEAAEGGAERLIIVTSEGKDSVVAHFVEDLVLEGTLEARGKTSMLEKVRRAPALI
                     KVESVVQAEPLGLGHAVSCVEPALMPDEDAIAVLLPDDLVLPTGVLETMSKVRAKRGG
                     SVLCAIEVPREDISAYGVFDVETVPDTNNPNVLRVKGMFEKPKAENAPSLYAAAGRYV
                     LDRKIFDALRRVPRGAGGEIQLTDAIALLINEGHPVHVVVHRGDRHDLGNPGGYLKAA
                     VDFALKRDDYGPELRRWLVE"
     gene            976..1536
                     /locus_tag="VGP27_12765"
     CDS             976..1536
                     /locus_tag="VGP27_12765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV7852205.1"
                     /translation="MRAVILAARHAVSPEVHDAEAEALCGHLPDVVRHGSTVCAYVPS
                     RFEPGSIALLDILLRLGNRVLLPVARSGADATPLPMQWGRYQPGRLVPARLGLQEPQK
                     PWLPAEAVAEATVVLVPALAVDRAGARLGRGAGFYDRSLPLALPGARLVAVVRDDELV
                     AEIPAEPHDVPMTHALTPRRGLVELG"
     gene            complement(1547..>1735)
                     /locus_tag="VGP27_12770"
     CDS             complement(1547..>1735)
                     /locus_tag="VGP27_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7852206.1"
                     /translation="NLLAVAGQANFDKAFRDATAWLPPNAAFRCEFVARQIDVKAAYA
                     LWLSKNEKRAMEDVLARC"
BASE COUNT          268 a          607 c          574 g          286 t
ORIGIN      
        1 ttccaccaac caccgccgca gttcggggcc gtagtcgtcg cgcttcaacg caaagtcaac
       61 cgcagccttg aggtacccgc cgggatttcc caggtcgtgt cgatcgccgc gatgcaccac
      121 gacatggacc ggatggccct cgttgatcag cagcgcgatc gcatcggtca actggatctc
      181 gccgcccgcc ccgcgcggca cccgacgcag cgcgtcgaag atcttgcggt cgagcacgta
      241 cctccccgcg gcggcataca gcgacggagc gttctcggcc ttgggcttct cgaacatccc
      301 cttgacccgt aacacgttgg gattgttggt gtctgggacg gtctcgacat cgaagacgcc
      361 gtaggcgctg atgtcttccc tcggcacctc gatcgcgcag agcaccgaac cgccacgctt
      421 ggcccgcacc ttcgacatcg tctccagcac gccggtcggc aacacaaggt cgtctggcag
      481 cagcaccgcg atggcgtcct cgtcgggcat caacgcgggt tcgacgcagc tgaccgcgtg
      541 cccgagacca agaggttcgg cctgcaccac cgactccacc ttgatcaggg cgggtgcgcg
      601 gcggaccttc tccaacatcg acgtcttgcc gcgggcctcc agcgtgccct cgaggacgag
      661 gtcctcgacg aagtgcgcga cgacgctgtc cttgccttcg gaggtgacga tgatcagtcg
      721 ctcggcgccg ccctccgcgg cttcggcggc caccagttcg atgcccggcg tgtcaactac
      781 aggcagcaac tccttgggca ccgtcttggt cgcgggcagg aaacgggttc ccaatccggc
      841 agcggggacg accgccgtcc gcggtatcga gacgtgggcg cgtgtcatcg ttcaacacta
      901 tcgccctcga gttatgtgcc gcctgtgtcc gcgccgtgtc atcgtggacg cgtgggctgc
      961 aggagtaaag cggagatgcg ggcggtgatc ctggcggccc gtcacgcagt cagccctgag
     1021 gttcacgacg ccgaggccga ggccctgtgc ggccaccttc ccgacgtggt ccgccatgga
     1081 tcgacggtct gcgcctacgt gcctagcaga ttcgagccgg ggtcaattgc gttgctggac
     1141 atactgttac ggctcggcaa tcgggtgttg ctgcccgtgg cgcgcagcgg cgccgacgcc
     1201 acgcccctgc cgatgcagtg ggggcggtat caacccggcc ggctggtccc ggcccggctg
     1261 ggactgcaag aaccgcagaa gccatggctg ccggccgagg cggtggccga ggcgacggtg
     1321 gtgctcgtgc ccgcgctggc cgtcgaccgg gccggggcgc ggctgggccg tggcgcgggc
     1381 ttctacgacc ggagcctgcc gctcgccctt cccggcgcac gcctggtggc ggtggtccgc
     1441 gacgacgaac tggtcgccga gataccggcc gagcctcacg acgtgccgat gacccacgcg
     1501 ctcacgccgc gccgcggcct cgtcgaactg ggctgagaac gctgagtcaa caccgagcga
     1561 gcacgtcctc catcgcccgc ttctcgttct tcgacagcca cagcgcgtag gcggccttca
     1621 catcgatctg ccgcgcgacg aactcgcagc ggaacgccgc attgggtggc aaccaggcgg
     1681 tggcatcgcg aaaagccttg tcgaagttgg cctgcccggc gacggccagc aggtt
//
LOCUS       DASYLX010000526         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3645184,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000526 DASYLX010000000
VERSION     DASYLX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645184"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="VGP27_12775"
     CDS             <1..95
                     /locus_tag="VGP27_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852207.1"
                     /translation="VKRLRSKIESDPANPVHLVTVRGLGYKLEG"
     gene            complement(193..969)
                     /locus_tag="VGP27_12780"
     CDS             complement(193..969)
                     /locus_tag="VGP27_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852208.1"
                     /translation="MTAEHPGEVELDFAREWVEFYDPDNPEHLIAADLTWLLSRWTCV
                     FGTPACHGTVKGRPDDGCCSHGAFLSDKDDRKRLDAAVKMLTDDDWEFRDKGLGRKGY
                     LEMDEYEDKPNWRTRKYKGACIFLNRPGFKAGIGCALHTKAIQLGVEPLTMKPDVCWQ
                     LPIRRTQEWVTLADGTEILKTWITEYDRRGWGEGGADLHWYCTGDPNAHVGSKQVYQS
                     YAPELTELLGEKAYAELAAMCKRRNGLGLIAVHPATRAAE"
     gene            1138..>1617
                     /locus_tag="VGP27_12785"
     CDS             1138..>1617
                     /locus_tag="VGP27_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852209.1"
                     /translation="MRLGVLDVGSNTVHLLVVDARRGGHPTPMSSTKASLRLAEAIDS
                     SGKLTRKGADKLIDTIDEFSKIAASSGCVELMAFATSAVRDARNSEDVLARVLAETGV
                     ALQVLSGVDESRLTFLAVRRWYGWSAGRIINIDIGGGSLELSNGVDEEPEVALSLPLG
                     "
BASE COUNT          265 a          514 c          536 g          302 t
ORIGIN      
        1 acgtcaagcg gttgcgctcg aaaatcgagt ccgacccagc caatccggtg cacctggtga
       61 cggtgcgcgg tctgggctac aagctggagg gctgaggacg acgatcaagc ggcgaggaac
      121 gagccgcgtt gtgggggtac cacccacgcg aagcgtagtg ggagagtccg accagatagg
      181 gggctgagga cgctactcgg cggcgcgggt ggccgggtgt acggcgatca gccctaaacc
      241 attgcgccgc ttgcacattg ccgccagctc ggcgtaggcc ttctcgccga gcagttcggt
      301 cagctcgggt gcgtaggact ggtagacctg tttggatccg acatgcgcgt tcgggtcgcc
      361 ggtgcagtac cagtgcagat cggctccgcc ctcgccccag ccgcggcggt cgtactcggt
      421 gatccacgtc ttgaggatct cggtgccgtc ggccagcgtc acccactcct gggtacgtcg
      481 gatcggcagc tgccaacaca cgtccggctt catcgtcagc ggttcgacac ccagttggat
      541 ggccttcgtg tgcagcgcgc agccgatgcc tgccttgaag ccggggcggt tgaggaagat
      601 gcaggcgccc ttgtattttc gggttcgcca gttcggcttg tcctcgtatt cgtccatctc
      661 gagatagccc ttgcggccca atcccttgtc ccggaactcc cagtcgtcgt cggtcagcat
      721 cttcaccgcc gcgtcgaggc gcttgcggtc gtccttgtcg gacaggaacg caccgtggga
      781 acagcagccg tcgtcggggc gtcccttgac ggtgccgtga cacgccggtg tgccgaacac
      841 acacgtccac cgggacagca gccacgtcaa atcggcggcg atcaggtgct cggggttgtc
      901 ggggtcgtag aactccaccc attcacgggc gaaatccagt tcgacctcac cgggatgctc
      961 tgcggtcacg gtttcaacgc tagcgccgaa ttccgccgaa cgctaagttg ccacggccgc
     1021 gaacaccaac atatctcggt gatcatgaac cgcccggcga cctgtgggat gttctatcgg
     1081 cacgccacac cgcggtctgc tcacgaagtt cactcccaga cccgttaggt tgaacgtgtg
     1141 cgacttggtg tgctcgacgt gggcagcaac accgtccacc tcttggtggt ggacgctcgc
     1201 cgtggcggcc atccgacacc gatgagttcc acgaaggcgt cgctgcggct ggccgaggcg
     1261 atcgattcct cgggcaagtt gactcgcaag ggtgccgaca agctcatcga cacgatcgac
     1321 gagttctcca agatcgccgc cagttcgggc tgcgtggagt tgatggcgtt cgccacctcc
     1381 gcggtgcgtg atgcccgcaa ctccgaggac gtgctggccc gcgtgctggc cgagaccggc
     1441 gtggcccttc aggtgctcag cggggtcgac gaatcgcggc tcaccttcct ggcggtgcgc
     1501 cggtggtacg gctggagcgc gggccgcatc atcaacatcg acatcggcgg cggatcgctg
     1561 gaactgtcca acggcgtcga tgaggagccg gaggtggcgc tgtcgctgcc tctgggc
//
LOCUS       DASYLX010000527         4974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3645255,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000527 DASYLX010000000
VERSION     DASYLX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645255"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VGP27_12790"
     CDS             <1..1268
                     /locus_tag="VGP27_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7852210.1"
                     /translation="RVSSIDVTRDGVRAVLKLQSSTKVPSDVSAAVHSRSAVGEQFVE
                     LSPRGGQGDTSRALADGDVIPVGRTQVPPDIGGLLDATNRALQEIPQDNLRTVVDEAD
                     KAVADLGPELSRIVDGSTSLAREAAKNQDSITALIDQSPPVLNSQVQTADSIAKWADR
                     LASITSQLKAQDAAFADLLNIGGPALEEGRALLDRVAPALPVLLANLVSLGEIAVTYR
                     SDIEQLLVLFPQGTAVMSAIVAANANTKQDYKGVYLDFNLNLNLPPPCNTGFLPVRQQ
                     RSPSDVDYPERPAGELYCRVPQDSSLNVRGVRNIPCEGNPAKRAPTAEMCESNDEYVP
                     LNDGFNWKGDPNATLSGQGVPQYAQGSDPRLPPPAGTAAAPPPAVAFAPYDPATGDYV
                     GPDGSRHTQADLAHPQNKTWQSMLIPPAS"
     gene            complement(1265..2227)
                     /locus_tag="VGP27_12795"
     CDS             complement(1265..2227)
                     /locus_tag="VGP27_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7852211.1"
                     /translation="MATEHVVVVVVFDGMKLLDAAGPAEVFAEANRFGASYRLVVASV
                     DGADVTTSIGTRFPATTHIDAVEFADTAIVSGGDELVGKPIDPDLVAAVQRLRSRSRR
                     MASICTGSFILARAGLLAGRRATTHWRHTRLLARAYPDVTVEADAIFVGDGDLYTSAG
                     VSAGIDLALNLVEDDYGADLVREVARSLVVFLKRSGGQSQFSAAVEAEPPKRSALRGV
                     TDAIAANPAANHNVKDLALQASLSTRQLTRLFSSELGTTPARYVEAIRIDAARAALDA
                     GSSVGDAARTAGFGSPETLRRVFVSELGISPRAYRDRFRSTVGG"
     gene            2311..2952
                     /locus_tag="VGP27_12800"
     CDS             2311..2952
                     /locus_tag="VGP27_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7852212.1"
                     /translation="MTAHTIAGVTIPDSPLVRDTTEYIRDVENDLLFDHSRRVFLFGA
                     LAGRRRGLDPDLELLYVGAMFHDVGLTQGYRESMARFEVDGANAARDFLLARGTDEHT
                     VRKVWLSIALHTTPGIPEFLDPEIALVTAGVETDVLGIGRDELSSDALAAVTGAHPRP
                     DFKRRILQAFTDGNVHRPGSTFGNVNADVLAHYDPTFVRDDFVEIILDNKWPE"
     gene            3011..3454
                     /locus_tag="VGP27_12805"
     CDS             3011..3454
                     /locus_tag="VGP27_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7852213.1"
                     /translation="MAVSESREVVIEATPQEILDVIADVESAPDWSSQHQGAEVLDTV
                     DNGRPGRVRLKLKTMGIADEQVVQYEWTDTTAGWTLISSSQLKKQDAKYTLTSEGDKT
                     RVKFEISVDPAVPIPGFVLKRAMKGGLESATDGLRKQVLKVKKGS"
     gene            3495..3938
                     /locus_tag="VGP27_12810"
     CDS             3495..3938
                     /locus_tag="VGP27_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7852214.1"
                     /translation="MAITEARDVTIEATPDEIMDVLVDLESLTEWSSTHQKVEVLERD
                     DQGRPAKSRQVVKIVGVSDEQVLDYTVHDDGVSWTLVSSKQQRAQRGRYTLTSKGDST
                     DVHFELTVDPLVPLPGFLVKRGAKGLMETATDGLRKRVLKIKKGG"
     gene            4022..>4974
                     /locus_tag="VGP27_12815"
     CDS             4022..>4974
                     /locus_tag="VGP27_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7852215.1"
                     /translation="MGAVGAAMSSPGIGGILICGPAGVGKSRVAREALSAAASQGRET
                     RWTVGTSSARAIPLGAFTAWAPSGVTDTVQLLRGVIEALTAETSSAKVIVGVDDAHLL
                     DELSTFVVHQIVARGAAKVILTARDDVPIPSAVQEIWKAAPFDRLDLKPLSPGETTKL
                     LSATLGGSVAPEAAERLWKLTHGNVLYLRNIVEQEVADGRLVKERGCWRWTGDPVMPP
                     GLAELIESRIGDLPAPVSDVIDVVAVGEPLELALLSRLTDPAAIEEADTRDLITLEPA
                     GHGIEVRVAHPLYGEVRRRRAPVTRLRRLRGLVATELATSD"
BASE COUNT          882 a         1616 c         1672 g          804 t
ORIGIN      
        1 gcagggtctc ctccatcgac gtcacccgcg acggcgtccg tgcggtcctg aagctgcagt
       61 cgtcgacgaa ggtgccctcc gatgtatcgg ccgcggtgca cagccggtcg gccgtgggag
      121 agcagttcgt ggaactgagt ccgcgagggg gacagggcga cacgtcgcgg gcgctggccg
      181 acggcgacgt catccccgtc ggcaggacgc aggttccgcc cgacatcggt ggcctgctgg
      241 acgccaccaa tcgcgcgctg caggagattc cgcaggacaa tctccgcacc gtcgtcgacg
      301 aagcggacaa ggccgtcgcc gacctcggtc ctgaactgtc ccgcatcgtc gacggatcga
      361 catcgctggc gcgggaggcg gccaagaacc aggactcgat caccgcgttg atcgaccagt
      421 ccccgccggt gctgaactct caagtgcaga ctgcggattc gattgcgaaa tgggccgatc
      481 ggttggcgtc catcaccagc cagctcaagg cccaggacgc ggcgttcgcc gacctgctca
      541 acatcggcgg gcctgcgctc gaggaaggca gagcgctgtt ggaccgcgtc gcgccggcct
      601 tgccggtgct gctggccaac ctggtgagcc tgggtgagat agcggtgacc taccggtccg
      661 acatcgaaca actgctggtt ctctttccac aggggacggc cgtcatgtcg gcgatcgttg
      721 ccgcgaacgc gaacacgaag caggactaca agggcgtcta cctcgacttc aacctcaacc
      781 tcaacctgcc gccaccctgc aacaccggat tcctgccggt gcggcaacaa cgttcgcctt
      841 ccgacgtcga ctaccccgag cggcccgcgg gcgaactgta ctgccgggtg ccgcaggact
      901 catcgctgaa tgtccgtggc gtgcgcaata ttccgtgtga gggcaaccca gccaagcggg
      961 cgccgacggc ggagatgtgc gagagcaatg acgagtacgt gccgttgaac gacggcttca
     1021 actggaaggg cgacccgaac gcgacgctgt ccggtcaggg tgtgccgcaa tatgcccagg
     1081 gttcggatcc gcggctgccg ccgccggcgg gcaccgcggc tgcgccgcca ccggcggtcg
     1141 cgttcgcgcc ctacgatccg gcgaccggtg actacgtcgg gcccgacggc agccgccaca
     1201 cgcaggccga cctcgctcac ccgcagaaca agacctggca gtcgatgctg atcccgccgg
     1261 cgtcctagcc gccgaccgtt gaccggaacc ggtcacggta agccctcggt gagatgccga
     1321 gctcgctgac gaacaccctg cgcagcgtct cggggctgcc gaagcccgca gtccgtgcgg
     1381 catcgccgac ggagctgccc gcatcgagag ccgcgcgtgc tgcgtcgatc ctgatggctt
     1441 cgacgtaacg cgccggcgta gtgccgagct cggaggagaa cagccgagtc agttgtcgtg
     1501 tgctcaaaga agcttgtaac gcaaggtctt tgacgttgtg atttgcggcc gggttggctg
     1561 cgatggcatc ggtcacgcca cgaagggccg atcgcttagg cggctcggcc tccaccgctg
     1621 cggagaactg cgactggccg cccgaacgct tgaggaacac caccagcgac cgtgccacct
     1681 cacgaaccag atcggcaccg tagtcgtctt cgacgaggtt cagggcgaga tcgataccgg
     1741 ctgagactcc cgccgaggtg tacaggtcgc cgtctccgac gaagatggcg tcggcttcga
     1801 ccgtcacgtc tgggtatgcc cgtgccagga gtcgggtgtg acgccaatgc gtggtcgccc
     1861 ggcggccggc caacagaccc gcacgcgcca gaatgaacga ccccgtacag atcgacgcca
     1921 tccggcggct gcgcgatcgg agcctctgca ccgccgcgac caggtccggg tcgatgggct
     1981 tgccgaccaa ctcgtcgccg ccggacacta ttgcggtgtc ggcgaactcg accgcatcga
     2041 tgtgcgtcgt cgccgggaag cgcgttccga tcgaggtcgt cacgtcggcg ccgtcgacgg
     2101 acgccaccac caaccggtag gacgcgccga agcggttcgc ctccgcgaag acctcggcgg
     2161 gtccggcggc gtcgagcaac ttcatgccgt cgaagaccac gacgacaacg acatgctcgg
     2221 tggccatcac cccactgtat gtcgctttct gtgggaacga cggcggatcg gacacgggtc
     2281 aggcagtgtc ggcgagctga cactgttgtt atgactgcac acaccatcgc cggggtcacg
     2341 atccccgaca gcccgctggt gcgggacacc accgagtaca tccgtgatgt ggagaacgac
     2401 ttgctgttcg accactcgcg tcgcgttttc ctcttcggcg cacttgcggg tcggcgccgc
     2461 ggcctcgacc ccgacctgga actgctctat gtcggggcaa tgttccatga cgtcggcctg
     2521 acgcagggct atcgcgaatc gatggcgcgt ttcgaggtcg acggcgccaa tgctgcgcgc
     2581 gacttcctgc tggcacgcgg caccgacgaa cacaccgtgc gcaaggtgtg gctgagcatt
     2641 gcgctgcaca ccacccctgg catccccgaa ttcctcgacc ccgagattgc tctcgtcacc
     2701 gcgggcgtag aaaccgatgt gctcggtatc gggcgcgacg agctgtcctc cgacgcgctt
     2761 gccgcggtga cgggcgctca tcccaggccc gacttcaaac gccgaatcct gcaggcattc
     2821 accgacggca atgtccatcg gccgggcagc acgttcggca acgtcaacgc cgacgtcctt
     2881 gctcactacg acccgacatt cgtccgcgac gatttcgtgg agatcatcct cgacaacaaa
     2941 tggccggagt gactgtccga ttcgggcgat agacccccgg cagcgggtga ggggcaacta
     3001 tggtcgcgcc atggcagtca gcgagtcccg cgaagtggtc atcgaagcaa cgccccagga
     3061 aatactcgac gtcatcgccg acgtggaatc cgcaccggac tggtcgtcgc agcaccaggg
     3121 ggccgaggtg ctcgacacgg tggacaacgg cagacccggc cgggtgaggc tcaagttgaa
     3181 gaccatgggc atcgccgacg agcaggtcgt gcagtacgag tggaccgaca cgaccgccgg
     3241 ctggaccttg atcagctcga gccagctcaa gaagcaggac gcgaagtaca cgctgacttc
     3301 ggagggcgac aagacccggg tcaagttcga gatcagcgtg gacccggcgg tgccgatccc
     3361 cggcttcgtg ctgaagcgag cgatgaaggg cggcctcgaa tcggccaccg acggcctccg
     3421 taagcaggtg ctcaaggtga agaaggggtc atagcggagg tttcgggttc cgccgcggtg
     3481 ggcaaaatcc tcccatggcc atcaccgaag ctcgcgacgt gacgatcgag gcgacccccg
     3541 acgagatcat ggacgtgctc gtcgacctgg aatcgctgac cgagtggtcc tcgacccatc
     3601 agaaggtgga ggtactcgaa cgagacgacc aggggcgacc ggccaagtcg cgtcaggtgg
     3661 tcaagatcgt cggtgtgagc gacgaacagg tgctggacta caccgttcac gacgatggcg
     3721 tgagctggac tctggtcagc tcgaaacagc aacgtgcaca acggggccgc tacacgttga
     3781 cttccaaggg cgattcgacc gacgtgcact tcgagctgac ggtggatccg ctcgtgccgt
     3841 tgcccggctt cctggtcaag cgaggcgcga agggcctgat ggagacggcc accgacggcc
     3901 tgcgcaagcg ggtgctgaag atcaaaaagg gcgggtaggc gaagtcgcgg tattcccgcg
     3961 cggcggcttc taggctcatc gtgtgcattt gccgtggccg ttaattggcc gctccgagga
     4021 gatgggcgcg gtcggggccg ccatgtcgtc ccctggtatc ggtggcattc tcatctgcgg
     4081 cccggcaggc gtgggaaaga gtcgggtcgc gcgggaggcg ctttcggctg ccgcctcgca
     4141 aggccgtgaa acccgatgga cggtagggac ctcgtcggcg cgggcgattc cactgggcgc
     4201 tttcaccgcg tgggcgccgt cgggtgtgac cgacaccgtt caattactgc gcggggtcat
     4261 cgaagctctg accgccgaga cctcgagcgc caaggtcatt gtcggggtag acgacgcgca
     4321 cctgctcgac gagctgtcga cgttcgtggt ccaccagatc gtggctcgcg gcgctgccaa
     4381 ggtgatactc acggcccggg acgacgtgcc gattccctct gcggtacagg agatctggaa
     4441 ggccgcaccg ttcgaccgtc ttgacctcaa gccgctgtct cctggcgaaa ccacgaagct
     4501 gctgtcggcg acccttggcg ggtcggtggc gcccgaagcc gctgagcggc tatggaagct
     4561 gacgcacggc aatgtgctgt atctgcgcaa catcgtcgag caggaagtcg ccgacggccg
     4621 actggtgaag gagcgcgggt gctggcgttg gaccggcgac cccgttatgc cgcccggtct
     4681 ggccgagctg atcgagtccc gcatcggtga cctgcccgcg ccggtgagtg acgtgatcga
     4741 tgtggtggcg gtcggggagc cgctcgagct cgcactgctg tcgcggctga ccgaccctgc
     4801 cgctatcgaa gaggcggata cccgggatct catcacgctg gagcccgccg gccacggtat
     4861 cgaggtgcgg gtggcgcatc cgctgtacgg cgaggtgcgc cgcaggcgcg cgccggtgac
     4921 ccggcttcga cggcttcgcg gactggtcgc cacggaactt gcgacgtcgg atga
//
LOCUS       DASYLX010000528         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3097876,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000528 DASYLX010000000
VERSION     DASYLX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097876"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VGP27_12820"
     CDS             complement(<1..975)
                     /locus_tag="VGP27_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852216.1"
                     /translation="MTVPTSDVILDPYDYHFHEDPYPYYKRLRDDAPLYYNEELGFWA
                     LSRHHDVREGFRNSTTLSTRDGVSLDPISRGPHASKTMSFLAMDDPGHLRLRTLVSKG
                     FTPRRIRELEPRVTELAIQHLDAMLERAAAGETVDYVAEFAGKLPMDVISELMGVPVE
                     DRDRIRAMADGVMHREDGVNDVPQSAIEASINLMVYYQEMITDRRRKAADDLTTALLE
                     AEIDGDRLTDDEVLGFLFLMVIAGNETTTKLLANAAFWGHRNPDQLAPLYQDLDTIPL
                     WVEETLRYDTSSQLLARVVAGELNLYDTTIPDGDILLLLPGSAHRDERV"
     gene            complement(1007..1603)
                     /locus_tag="VGP27_12825"
     CDS             complement(1007..1603)
                     /locus_tag="VGP27_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852217.1"
                     /translation="MSSDAVVVVPDPSGETPRNRRQEETFRKVLAAGVEMLRESSYAD
                     LTVRAVAARAKVAPATAYTYFSSKNHLISEVYLDLMKQVPYFTDVNDSTPARVEKALR
                     AMTLMIADEPEVAAACTTALLSNNDAAVRTVRDGIGAEIHRRIRSAMGPDADPRTVSA
                     LEMAYFGALVHAGSGYVSYREIADRLTYVVGLILGEDR"
     gene            1789..>2706
                     /locus_tag="VGP27_12830"
     CDS             1789..>2706
                     /locus_tag="VGP27_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7852218.1"
                     /translation="MGMLADRQSQLLIDGKLVAGSAGTFPTVNPATEEVLGVAADAGA
                     DDMDRAIGAARRAFDETDWSTDTELRVRSIRQLQDVLTEHVEDLRQITIAEVGAPVML
                     TSMAQLEGPIADLSFCADTAESYSWTTDAGIASPMGIKTHRTIAKEAVGVVGAITPWN
                     FPHQINLAKIGPALAAGNTVVLKPAPDTPWCAAAVGQLIAEHTDIPPGVINIVTSSDH
                     AVGALLSKDPRVDMVSFTGSTATGRAVMADGAATLKKVFLELGGKSAFLVLDDADLAG
                     ACSMSAFTAAMHAGQGCAITTRLVVPRARY"
BASE COUNT          473 a          885 c          874 g          474 t
ORIGIN      
        1 cacccgctcg tcgcggtgcg ccgagccggg cagcaacaac aggatgtcac cgtcgggaat
       61 ggtggtgtcg tagaggttca actcccctgc gaccacgcga gccagcagct gactggatgt
      121 gtcgtagcgc agagtctcct ccacccacaa cggaatcgtg tcgagatcct gatacagcgg
      181 agccagctga tcggggttcc gatggcccca gaacgccgcg ttggcaagca acttggtggt
      241 cgtctcgttg ccggcgatca ccatgaggaa caagaacccg agcacttcgt cgtcggtcag
      301 ccggtcaccg tcgatctcgg cttcgagtag ggcggtcgtc aagtcgtccg ccgctttcct
      361 gcgacggtcg gtgatcatct cctggtagta gaccatcagg ttgatggatg cctcgatcgc
      421 ggactgaggt acgtcgttga caccgtcttc acggtgcatg acgccgtcag ccatggcccg
      481 gatgcggtcc cggtcttcca ccggcacgcc catcaactcg gagatgacat ccatcggcag
      541 cttgcccgcg aattccgcta cgtagtcgac cgtttcaccg gcggcggccc tctccagcat
      601 cgcgtcgagg tgctggatcg ccagttcggt gacgcgcggt tcgagctccc ggattcgccg
      661 cggcgtgaac cccttcgaga ccaatgtgcg caggcgcaag tgtccgggat cgtccatcgc
      721 gaggaacgac atggtcttgc tcgcatgcgg cccgcgggag atcggatcga gtgagacgcc
      781 gtcgcgggtg gacaacgtcg tgctgttgcg gaagccttcc cgcacgtcgt gatggcgcga
      841 cagcgcccag aagccgagtt cctcgttgta gtagagcggg gcgtcgtcgc gcaaccgctt
      901 gtaatagggg tacggatctt cgtggaagtg gtagtcgtag gggtcgagga tcacgtcgct
      961 ggttggcacg gtcaccgaga atcccggtcc tctcgaaggc ttctggtcat cgatcctctc
     1021 cgaggattag gccgaccacg taggtgagtc ggtcggcgat ctcgcgatag ctgacatagc
     1081 cgctgccggc atgaaccaga gcaccgaagt acgccatctc gagcgccgac actgtgcgcg
     1141 ggtcggcgtc ggggcccatc gccgagcgga tgcggcggtg aatctcggcg ccgatgccgt
     1201 cgcgaaccgt gcggacggcc gcgtcgttgt tgctcagcag cgccgtcgtg caggcagccg
     1261 cgacttcagg ctcgtcggcg atcatcagag tcatcgcccg cagtgccttc tcgacccggg
     1321 ccggcgtgct gtcgttgaca tcggtgaaat agggcacctg cttcatcagg tcgaggtaga
     1381 cctcggagat cagatgattc ttcgacgaga agtaggtgta cgccgtcgcg ggcgccacct
     1441 tggcgcgtgc ggcgaccgcg cgcacggtga gatcggcgta ggacgactcc cgcagcatct
     1501 ccacacccgc ggcgagaacc ttgcggaacg tctcttcctg ccgtcggttg cgcggcgttt
     1561 ccccgcttgg gtcgggcacc acgaccacgg catcgctgga cacatgtcca agttatcgga
     1621 ccgatgcggc ggcaagcaag tgcggcggcc aaacccgcag tacaaaccgc atttgttgag
     1681 gtgtaccggc tcaacctcag cggtcggcct tgcttcgcgc accggtctgc ggttatcttc
     1741 gagtgaagtt agtcggacag ttgtccagca atcacgacgg gagtcgaaat ggggatgctg
     1801 gccgatcgcc agagtcaact gctcatcgat gggaaactgg tcgcaggcag cgcaggcacg
     1861 ttcccgacgg tcaacccggc caccgaggaa gtgctgggcg tcgccgccga cgcgggcgcc
     1921 gatgacatgg accgcgccat cggggccgcc cgtcgcgcat tcgacgaaac cgactggtcc
     1981 accgacaccg aactgcgtgt gcgcagcatc aggcaattgc aggacgtgct gaccgagcac
     2041 gtcgaagacc tgcgccagat cacgatcgcc gaggtcggcg cgccggtgat gctgacgtcc
     2101 atggcgcagc tggaaggtcc gatcgccgac ctcagcttct gcgccgatac cgccgagtcc
     2161 tactcgtgga ccaccgatgc tggtatcgcg tcgcccatgg gcatcaagac ccaccgcacg
     2221 atcgcgaagg aagccgtcgg cgtcgtcggc gccatcacac cgtggaactt cccgcaccag
     2281 atcaacctcg ccaagatcgg accggcgctg gccgcgggca acaccgtcgt gctaaaaccc
     2341 gcacccgaca ctccgtggtg cgccgccgcc gtcggccaac tcatcgccga gcacaccgac
     2401 ataccgccgg gtgtcatcaa catcgtcacc tcgagcgacc acgccgtcgg tgcgctcctg
     2461 tcgaaagacc cacgggtcga catggtttcg ttcaccgggt cgacggcgac cggtcgcgcc
     2521 gtgatggccg acggcgcggc gacgctcaag aaggtcttcc tcgagctcgg cggcaagtcg
     2581 gcgttcctgg tgctcgacga cgccgacctt gccggcgcat gctcgatgtc ggcgttcacc
     2641 gcagccatgc acgccgggca gggctgtgcg atcaccaccc ggcttgtggt gccgcgcgcc
     2701 cgctac
//
LOCUS       DASYLX010000529         7739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_245240,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000529 DASYLX010000000
VERSION     DASYLX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245240"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VGP27_12835"
     CDS             <1..646
                     /locus_tag="VGP27_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852219.1"
                     /translation="RIDILVHNAGNVRRASLKAMSYDDFEAVLDVHLRGAFHVVRQAF
                     PLMCDAGYGRIVLTSSIGGLYGNHEVANYAIAKAGVIGLSNVAAIEGAAEGVKSNVIV
                     PAAVTRMAEGIDTSAYPPMGPELVAPVVGWLAHETCSVTGEILLALAGRVARAVVAET
                     PGVYRPSWSMADVGEHRAAIRDASDPVVFPVVPDGHGDHIRYSFGMAAQGAVRG"
     gene            639..1814
                     /locus_tag="VGP27_12840"
     CDS             639..1814
                     /locus_tag="VGP27_12840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7852220.1"
                     /translation="MASQGPLTGVRVVDLTAMVMGPYCTQIMADMGADVIKVEPPEGD
                     NTRYISVGPAPGMSGVYMNVNRGKRAIVLDLRTESGRTALQALIRTADVFIHSMRSKA
                     IAKLGFGYDDVAAINPSIVYTNCYGYGRRGPYRDRPAYDDTIQAECGLPAVQEQLTGV
                     ANYVGTIMADKVAGLTALYATTMALFHRERTGEGQEVEVSMFETMASFMLVEHANGAM
                     FDPPLGPAVYPRTVAPNRRPYPTKDGHIAALIYNDKHWNAFVNAVRPPWATDLYATLE
                     LRANDIDTVYGLVAETMLQRTTDEWLDLFRELEIPAAPINTPGALFDHPHLNAVGLFE
                     TVETSYGPMRMPGVPTWFSRTPGRIAGPTRELGADTADVLRELGLDSADVGPAVGSG"
     gene            1836..2972
                     /locus_tag="VGP27_12845"
     CDS             1836..2972
                     /locus_tag="VGP27_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852221.1"
                     /translation="MGPAAADLRRELREMVKAHVPEGFLGAFTDNPADLETAQRFCRT
                     LADENLLCLSWPEEFGGGGASVWEQTVVREEMWAHHEPRGAQYMGVNWVGPIIMRHGT
                     EEQQRKHLPPIARGEVIWCQGFSEPEAGSDLASLRTTARPDGDGWLVSGQKIWTSYAT
                     MAQWCFLLARTHRGEKKQQGLTIFLVPMDDPAIQVRPIRTMLGPHHLNEVFFDDLRVS
                     RADVLGEVDQGWSIVAEVTSFERVGIARYARCERLLAAAPSVLGNRWEDLPAELRGRW
                     VRMLTHCRRARLLAYRVVSLQSSGRIQPGDAAAYRIAVTKLDQDSAEVLMEIAAEVSP
                     EDPGGRWFLGEVEDHWRYSQASTVSSGSIEMQRILLSRAMLAAK"
     gene            2978..3895
                     /locus_tag="VGP27_12850"
     CDS             2978..3895
                     /locus_tag="VGP27_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852222.1"
                     /translation="MILDLSEDAKEYGRQALRAFDAAGGDQLVQQAEAKPDTRDALVG
                     AVLTELGAWDLDARTDADGLEAAAALCRSAGYWAVPYPVAERLARPIDLDADGLIVVA
                     ANQPAGAIGGVEARWAAATVEGVRSAVVGHGVTGPAFVTTLELSETDRNGIGDVALGL
                     VLPCWTLLGMLDRALELTIAHVSLRKQFGQSLSTFQGVQFQLTDAEVERSGLEILAKY
                     ALWSAGSGKPEALVDALALRMSAIEAAEVVFRVCHQLHGAVGFCDETTLSWLSRYSQP
                     LRRLPFGLSATRDELTRRAGRSGLTGLYA"
     gene            3892..5148
                     /locus_tag="VGP27_12855"
     CDS             3892..5148
                     /locus_tag="VGP27_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852223.1"
                     /translation="MTADLDDFRATVRSWCEQHIPHGWRAAQTGVSDDEFARFQKAWF
                     AELHAAGFAVPHWPREWGGGLRTRGAETTPRTRGAETMLSVAQQIVLYQELAAHDAPR
                     LVLAFVGIHHAASTLLAAGTDDQRRRHLPAILDGEIWVQGFSEPEAGSDLAGLRTTAR
                     KDGDCYVVNGQKLWASGGMHADWCLLLARTDPEAPKRKGISYFLLDMTTPGVEVRPIR
                     NAIGDSHFCEIFLNDVTIPAANLVGAENAGWQVAQATLGAERGMTMLELAERLGNAGF
                     RWLVQSCPTDDPVIADRLARFETEIAGLRGLCRQLVENTEAGTVGPADASIVKLYYSE
                     LLQRMTDFGAETGGLPVHTELVKSASSGWESGSWMLDFIGSWEWTIPGGSSEIQRTII
                     AERGLGLPREPMPGRGPVSPRESGGA"
     gene            5148..6104
                     /locus_tag="VGP27_12860"
     CDS             5148..6104
                     /locus_tag="VGP27_12860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852224.1"
                     /translation="MTHEFAEFHAELRSVAGDLLAKDRAVGWPALVDAGWVGLEVREE
                     FGGAGVSFAEVAVICEEMGRAASANSYLGSAVLAVSTLNALQPSETRDRLLTDVSSGA
                     IRLAVVVESPEFVPDAEGADRVLSVTGGGVICAAAEVTPRPVLDETRRLATVSAKADA
                     SDVMRFQEDPIDARTRLLQRAAVAIACDSLGLSEAMLASTVAYAKVRYQFDRPIGSFQ
                     AVKHACADMAVKITVARRLVGAAVDAVAENAPDAGVCVAMAKSYACGTAVDIVGKAMQ
                     LHGGIGYTWESGIHVYLKRAALNRSLFGSPAAHRQELAKRYL"
     gene            6118..>7739
                     /locus_tag="VGP27_12865"
     CDS             6118..>7739
                     /locus_tag="VGP27_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7852225.1"
                     /translation="MGVPVYKRILDLFEAEGVNTLFGIPDPNFVHMFAEADARGWSVV
                     APHHELSAGFMAEAASRMTGKPGLCIGTLGPGVANISGAMMCALVENSPVIFLGGQRA
                     RITERRVRRGRIQFVQQEGLFTPSVKYSSSIEYADQTDEIVHEAIRRSMSGTPGPSYV
                     EFPSHVILEELNVGEAPEPSDYRLVNQGAGAREVAEAAKLIREAKSPILLVGHGVHTS
                     RTQGEVRELAELMACPVIQTSGGTSYIPGLQDRTFPYLFSPAANQAVEESDLCVALGT
                     ELGEPMHYGRTQHWAGNDANRKWVYVEQDPTAIGVNRRFDVPLVGDLRGVVPQLVEAL
                     RESPRSQSTNLETLVKADAAELTRVAEEAPSGRTPVHPARYVVEATKAFDEFAKDGIM
                     VRDGGATVIFQWTYSQTKPRDVIWNQNFGHLGTGLPYAVGASVAEGRKRPVMLLTSDS
                     AFLFHIAELETAAREGLPLVCVVGVDHQWGLEVGVYKRTFTQPSPQPGVHWSKDVRFD
                     KIAEGFGCHGEYVEKEDEIGPAIERAYASGKTA"
BASE COUNT         1301 a         2486 c         2686 g         1266 t
ORIGIN      
        1 ccgtattgac atcctcgtcc acaacgcggg caacgttcgt cgagcgtcgt tgaaagcgat
       61 gtcctacgac gacttcgagg ccgtgttgga tgtccaccta cgcggtgcct tccacgttgt
      121 gcggcaggct tttccgctga tgtgcgacgc gggttacggg cgcatcgtgc tgacctcgtc
      181 gatcggcggc ctttacggga accacgaagt ggccaactat gcgattgcca aggcgggcgt
      241 gatcggcctt tccaacgtcg ccgcgatcga gggtgccgcc gagggcgtca agagcaatgt
      301 catcgtgccc gccgcggtca ccagaatggc cgagggtatc gacacgtcgg cgtatccgcc
      361 gatgggcccg gagcttgtgg cgcccgtcgt cggatggctc gcgcacgaaa cctgttccgt
      421 cacaggggag attctcctag ctctggcggg ccgggtggca cgggcagtcg tcgcggaaac
      481 gccgggggtg tatcgcccgt cgtggtccat ggccgacgtc ggcgaacata gggcggcgat
      541 ccgcgacgcg tccgatcccg tcgtctttcc ggtggtaccc gacggccacg gcgaccacat
      601 ccggtacagc ttcggtatgg cggcacaagg agccgtccgt ggctagccaa ggcccgctga
      661 ccggggtgcg cgtcgttgac ctcaccgcga tggtgatggg cccgtactgc acccagatca
      721 tggccgacat gggcgccgac gtgatcaagg tcgagccgcc cgagggcgac aacacccgct
      781 atatctcggt cggtccggcg ccgggcatga gcggggtgta catgaacgtg aaccgtggta
      841 agcgtgccat cgtgctggac ctgcgaaccg aatcgggccg caccgcgctg caggcactga
      901 tacgaacggc tgatgttttc atccactcga tgcggtcgaa ggcgatcgcc aaactgggct
      961 tcggctacga cgacgtcgcc gccatcaacc cgtcgatcgt ctacacgaac tgctacggct
     1021 atggcaggcg cggaccgtat cgcgaccggc ccgcctacga cgacacgatc caggccgaat
     1081 gcggtcttcc cgccgtacag gaacagctga cgggcgtggc caactatgtc ggcacgatca
     1141 tggccgacaa ggtcgcgggc ctgaccgccc tctacgccac gacgatggcg ttgttccacc
     1201 gcgagcgaac cggcgaggga caagaggtcg aggtcagcat gttcgagacg atggcgtcgt
     1261 tcatgcttgt cgaacacgcc aacggcgcca tgttcgaccc tccgctcggg ccggccgtat
     1321 atccgcgaac ggtggcgccg aaccggcggc cgtatcccac caaggacggt cacatcgccg
     1381 cgctgatcta caacgacaag cactggaacg cgttcgtcaa cgccgtccgt ccgccgtggg
     1441 cgaccgactt gtacgccacc ctcgaactcc gcgcgaacga catcgacacc gtttacggtc
     1501 tggtcgccga gacgatgctg cagcgcacga ccgacgagtg gctggacctg ttccgcgagc
     1561 tcgagatccc cgcggcgccg atcaacacac cgggtgcgct gttcgaccat ccccacctca
     1621 acgccgtcgg gctgttcgaa accgtcgaaa cttcctacgg accgatgaga atgcccggtg
     1681 tgccgacgtg gttctcgcgg accccgggcc ggatcgcggg tccgacgcgc gagttgggcg
     1741 ccgataccgc ggacgtgctg cgcgaactgg gtctcgattc ggccgacgtc ggccccgcgg
     1801 tggggtcggg ataggggcgc tgcgtggact tcgagatggg acccgcggcc gccgaccttc
     1861 gccgcgagct gcgggagatg gtgaaggccc atgtgccgga aggcttcctg ggtgcgttca
     1921 cggacaatcc ggccgacctc gagacggcac agcggttctg caggacgttg gccgacgaga
     1981 acctgctgtg cttgtcgtgg ccagaggagt tcggcggcgg cggggcctcg gtctgggagc
     2041 agaccgtggt gcgagaggag atgtgggccc accacgagcc gcgcggcgcc cagtacatgg
     2101 gggtcaactg ggtcggaccg atcatcatgc ggcacggcac cgaagagcag cagcgcaagc
     2161 atctgccgcc gatcgcgcgc ggcgaggtca tctggtgcca gggtttctcc gagccggagg
     2221 cgggttccga cctggcatcg ctgcgcacca ccgccagacc ggacggtgac ggctggttgg
     2281 tcagcggtca gaagatctgg acgtcctacg ccaccatggc acagtggtgc tttttgctcg
     2341 cccgcaccca caggggagag aagaagcagc aggggctgac gattttcctt gtcccgatgg
     2401 atgatccggc gatccaggtg cgacccatcc gcaccatgtt gggtccgcac cacctcaacg
     2461 aggtgttctt cgacgacctg cgggtcagtc gggcagacgt gctcggtgag gtcgaccagg
     2521 gctggtcgat cgttgcggag gtgacgtcct tcgagcgggt cggcatcgcg aggtacgcgc
     2581 gatgtgaacg gctcctggcg gcggcaccgt cggttctcgg caaccgctgg gaggacctgc
     2641 ccgccgagtt gaggggccgg tgggtgcgca tgctcaccca ctgtcgccgc gcccgcctgc
     2701 tcgcatatcg cgttgtgtcg ctgcagagta gcggtcgtat ccagccggga gacgccgccg
     2761 cttaccgcat cgcggtgacc aagttggatc aggacagcgc cgaggtgctg atggagattg
     2821 ccgcggaggt gtcaccggag gatccgggcg gccgatggtt tctgggcgag gtcgaggacc
     2881 actggcgata ctcgcaggcg tcgacggtgt cctcgggcag catcgagatg caacgcatcc
     2941 tgctgtctcg cgccatgctg gcggcgaaat gagcgagatg atcctcgatc tgagcgagga
     3001 cgccaaagag tacgggcggc aggcacttcg ggcattcgac gcggccggcg gggaccaact
     3061 cgtccaacag gccgaggcca agcccgatac gcgggatgcc ctcgtcggtg cggtgctgac
     3121 cgaactgggc gcctgggatc tcgacgcgcg gaccgacgcc gacgggctgg aagcggccgc
     3181 ggcgctgtgc cgcagcgccg gctactgggc ggtgccgtat ccggtcgccg agcgcctcgc
     3241 gcggccgatc gatctcgacg ccgacggcct gatcgtggtc gccgcgaacc aaccagcagg
     3301 cgcgattggc ggagtcgaag cacgttgggc ggcagcgacg gtggagggcg tgcgaagcgc
     3361 agtcgtcggc cacggcgtca caggccctgc gttcgtcacc acgctcgagc tctcggagac
     3421 ggaccgaaac gggattggtg acgtcgcgct cggcctcgtg cttccgtgct ggacactgct
     3481 cggcatgctg gaccgcgcgc tcgagctgac catcgcgcac gtcagcctgc gcaagcagtt
     3541 cgggcagtcg ctgtcgacgt tccaaggcgt ccagttccag ctcaccgatg ccgaagtgga
     3601 acgcagcggc ctggagatcc tcgccaagta cgcgctgtgg agcgccggtt ccggcaagcc
     3661 ggaagcactc gtcgacgcgc tggcgcttcg gatgtccgcc atcgaagcgg ccgaggtggt
     3721 cttccgggtg tgccatcagc tgcacggcgc cgtcggtttc tgcgatgaga ccacgctgtc
     3781 gtggctgtcc cgctacagcc agccgttgcg gcggttgccg ttcgggttgt ccgcgacccg
     3841 cgacgaactg acccgtcgcg cggggagaag cggtctcacg gggttgtacg cgtgacggcc
     3901 gatctcgacg acttccgcgc gacggtgcgg tcgtggtgtg agcaacacat cccgcacggc
     3961 tggcgcgcgg ctcaaaccgg cgtcagcgac gacgaattcg cccgttttca gaaggcatgg
     4021 ttcgccgagc tgcacgccgc gggcttcgcc gtcccgcact ggccgcggga gtgggggggt
     4081 gggctgcgca cgcgaggagc agagacgacg ccgcgcacgc gaggagcaga gacgatgctg
     4141 tccgtcgccc agcagatcgt gctgtatcag gagctcgccg cccacgacgc accgcggctg
     4201 gtgctcgcgt tcgtcggcat ccatcacgcc gcgtcgacgc tgctcgccgc gggaaccgat
     4261 gaccagcgtc gcaggcacct gcccgcgatc ctcgacggcg aaatctgggt tcagggcttc
     4321 tccgaacccg aggcggggtc cgacctggcc ggcctgcgga caacggcacg caaggacggc
     4381 gactgctatg tcgtcaacgg ccagaagctg tgggccagtg gcggcatgca cgcggactgg
     4441 tgcctgttac tggcccgtac cgaccccgaa gcgcccaagc gcaaaggtat ttcgtacttc
     4501 ctcttggaca tgaccacccc tggcgtcgaa gtgcgaccga tccgcaacgc catcggcgac
     4561 tcgcacttct gtgagatctt cctcaacgac gtcacaatcc ctgccgcgaa cctggtgggc
     4621 gcggagaacg ccggatggca ggtggcgcag gcgacactgg gcgccgagcg cgggatgacg
     4681 atgctggaac tcgcggagcg tctcggcaat gccggcttcc gttggctggt gcagtcgtgt
     4741 cccaccgacg atccggtcat cgccgaccgg ctcgcacgtt ttgaaacgga aatagccggc
     4801 ctgcgcgggc tgtgcaggca gctggtggag aacaccgagg ccggcacggt gggcccggcc
     4861 gacgcctcca tcgtcaagct ctactacagc gaactgctcc agcggatgac ggacttcggc
     4921 gccgagaccg gcggcctgcc ggtgcacacc gaactggtga agtcggcgtc cagcggatgg
     4981 gaatccggtt cgtggatgct ggatttcatc ggctcgtggg agtggacgat tccaggcggt
     5041 tcgagcgaga ttcaacggac catcatcgcg gagcgcggtc tggggctgcc gcgagagccg
     5101 atgcccgggc ggggccccgt gtcaccgcga gaatcaggcg gagcctgatg acacacgaat
     5161 tcgcagagtt ccacgccgag ctcaggtccg tcgcgggcga cctgctggcc aaggaccgcg
     5221 cggtcgggtg gcccgcgctc gtggatgccg gctgggtcgg tttggaagtg cgggaggagt
     5281 tcggcggcgc gggcgtaagt tttgccgagg tggccgtgat atgcgaggag atgggccgcg
     5341 cggccagcgc gaacagttat ctgggcagcg ccgtgcttgc ggtgagcacc ttgaatgcgt
     5401 tgcaacccag cgagacccgc gaccggctcc tcaccgatgt ctcgtcgggc gccatccggt
     5461 tggccgtcgt ggtcgaatcg ccggagttcg tccccgacgc cgagggcgca gaccgggttc
     5521 tgtcggtcac cggcggtggc gtcatatgcg cagccgcgga agtcacgcca cgaccggtgc
     5581 tcgatgagac ccgccggctg gcgacggtgt cggcgaaggc cgatgcgagc gatgtcatgc
     5641 ggttccaaga agaccccatc gacgcgcgta cacgactgct tcagcgcgcc gcggtcgcga
     5701 tcgcctgcga cagcctcggc ctctccgagg ccatgctcgc ctcgacggtg gcctacgcca
     5761 aggtccgata ccagttcgat cgtccgatcg ggtcgttcca ggcggtcaag cacgcgtgtg
     5821 ccgatatggc cgtcaagatc acggtggccc gccgactcgt cggtgcagcc gtcgatgcgg
     5881 tcgcggagaa cgcgccggat gccggcgtct gcgtcgcgat ggccaagtcc tacgcctgcg
     5941 gtacggccgt cgacatcgtc ggcaaggcga tgcagctgca cggtggcatc ggctacacgt
     6001 gggagagcgg catccatgtt tacctcaagc gcgccgcact caaccgttcg ttgttcggtt
     6061 caccagcagc tcaccgccaa gagctcgcaa aacgctacct ataaaaggag ttccacgatg
     6121 ggtgtccccg tatacaaacg cattctcgac ctcttcgagg ccgagggcgt caacacgctg
     6181 ttcggcattc ctgacccgaa cttcgtgcac atgttcgccg aagccgacgc gcggggctgg
     6241 tcggtcgtcg cccctcacca cgagctgagt gccgggttca tggccgaggc ggcgtcgcga
     6301 atgacgggta agccggggct gtgcatcggc acgctcgggc caggagtcgc caacatctcc
     6361 ggcgcgatga tgtgcgcgtt ggtggagaac tcgccggtga tcttcctcgg cggccagcga
     6421 gcccgcatca ccgagcgccg ggtccgccga ggacgcatcc agttcgtcca gcaggagggc
     6481 cttttcacgc cgtcggtcaa gtacagcagc tccatcgagt acgccgacca gaccgatgag
     6541 atcgtgcacg aggccattcg ccggtcgatg tccggcacac ctggtccgtc ctacgtcgag
     6601 tttccgtccc acgtcatcct ggaagaactc aacgttgggg aggcgcccga gccatcggac
     6661 tatcgcttgg tcaaccaggg tgcgggtgct cgagaggtcg cggaggccgc gaagctgatt
     6721 cgcgaagcca agagcccgat cctgttggtg ggccacgggg ttcacacgtc gcgcacccag
     6781 ggggaggtca gggaactcgc cgagctgatg gcctgcccgg tgatccagac atccggtggc
     6841 acgtcgtaca ttccgggact gcaggatcgg acattcccat acttgttctc cccggccgca
     6901 aaccaggcag tcgaggaatc cgacctgtgc gtcgcgttgg gtaccgaact cggtgagccg
     6961 atgcactacg gccggactca gcactgggcc ggcaacgatg cgaaccgcaa atgggtgtac
     7021 gtcgagcagg acccgaccgc catcggcgtc aaccgtaggt tcgacgtgcc gctggtcggc
     7081 gatctgcgcg gcgtcgtgcc gcaactcgtg gaggcgctgc gggagagccc gcgctcgcag
     7141 tcgaccaacc tggagaccct ggtcaaggcc gacgccgccg agctgacgcg ggtggcagaa
     7201 gaggctccaa gtggacgcac cccggtgcac ccggcgcgct acgtggtcga ggcgaccaag
     7261 gcgttcgacg agttcgccaa ggacggcatc atggtgcgcg acggtggcgc gaccgtcatc
     7321 ttccagtgga cttactccca gaccaagcca cgagatgtga tctggaacca gaacttcggt
     7381 catctgggca cgggtctgcc gtacgccgta ggcgcctcgg tcgccgaggg tcgcaagcgg
     7441 ccggtgatgc tgctgacgag cgactcggcg ttcctgttcc acatcgccga gctcgagact
     7501 gccgcgcggg agggcttgcc gctggtatgt gtcgtcggtg tcgaccatca gtgggggctc
     7561 gaggtcggcg tctacaagcg caccttcacc cagccgtcac cgcagccggg ggtgcactgg
     7621 agcaaggacg tgcgcttcga caagatcgca gagggcttcg gctgccacgg cgaatacgtg
     7681 gagaaggagg acgagatcgg cccggcgatc gagcgggcgt acgccagcgg caagaccgc
//
LOCUS       DASYLX010000530         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3317250,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000530 DASYLX010000000
VERSION     DASYLX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317250"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VGP27_12870"
     CDS             complement(<1..106)
                     /locus_tag="VGP27_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852226.1"
                     /translation="MSNQKPVGVAVLGLGNVGSQVVRIIEESAGDLAAR"
     gene            complement(110..1489)
                     /gene="lysA"
                     /locus_tag="VGP27_12875"
     CDS             complement(110..1489)
                     /gene="lysA"
                     /locus_tag="VGP27_12875"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV7852227.1"
                     /translation="MHHGGAPPRPQSSEQLLVLAPNVWPRNTVRADDGVVSIAGVKVT
                     DIADEFGTPAFIIDEDDFRSRCREIAEAFGGGENVRYAAKAFLCTEIARWVNEEGLSL
                     DVASGGELAVALHADFPAERIALHGNNKSVDELCSAVKAGVGHIVLDSMTEIERLDEI
                     SGAAGVVQDVLVRVTVGVEAHTHEFISTAHEDQKFGLSLASGAAMAAVRRVFETDHLR
                     LVGLHSHIGSQIFDVAGFEIAAHRVIGLLRDVVAEFGVDKTAQISTVDLGGGLGISYL
                     PQDDPPPVEDLAAKLSAIVANESAAVGLPTPRLVVEPGRAIAGPGTITLYRVGTVKDV
                     AVSPTASRRYVSVDGGMSDNIRTSLYGAEYDVRLISRAGEGGPMLGRIVGKHCESGDI
                     VVRDAWVSDDVQPGDLLGVAATGAYCYSMSSRYNLIGRPAVVAVRDGRARLILRRETV
                     DDLLSLEVR"
     gene            complement(1525..>1681)
                     /locus_tag="VGP27_12880"
     CDS             complement(1525..>1681)
                     /locus_tag="VGP27_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEV7852228.1"
                     /translation="YHRFYDSCRVLPQGDEEPNELHTARLALCQATRQVIENGLRILG
                     VTAPERM"
BASE COUNT          276 a          567 c          555 g          283 t
ORIGIN      
        1 tgcgtgcggc cagatccccg gcactctcct cgatgatgcg gaccacctgg ctgcccacgt
       61 tgcccaggcc caataccgct acaccgaccg gcttttggtt actcattgcc tacctcactt
      121 ccagactgag cagatcgtcg accgtctccc ggcgcagaat caggcgggcc cgcccgtcgc
      181 gcacggccac cactgcgggg cggccgatca ggttgtaacg gctcgacatg gagtagcagt
      241 acgcgcctgt tgccgccacg cccagcaggt caccgggctg cacgtcgtcg gacacccacg
      301 cgtcgcgcac cacgatatct ccgctttcgc aatgctttcc gacgatgcgg cccagcatcg
      361 ggccgccctc gccggcccgg gagatcagcc gcacgtcgta ttcggcgcca taaagcgatg
      421 tcctgatgtt gtcgctcatg ccgccgtcga cactgacgta gcggcgactt gcggtggggc
      481 tgacggcaac gtctttgacg gtgcccaccc ggtacagggt gatggtgccg gggccggcga
      541 tggcgcggcc cggctcgacc accaggcgcg gggtgggcag gccgacggcg gccgactcgt
      601 tcgccacgat ggcactgagc ttggctgcga ggtcctcaac tggaggcgga tcgtcctgtg
      661 gcagatacga aatgcccagt cccccaccga ggtcgacagt cgagatctga gccgttttgt
      721 cgacaccgaa ttcggcgacg acgtcacgca gcaggccgat cacccgatgg gcggcgatct
      781 cgaagccggc gacgtcgaag atctgggaac cgatgtggct gtgcagtccg acgagccgga
      841 ggtggtcggt ctcgaagacc cgccgcactg cggccatcgc ggcgccgctg gccagcgaca
      901 acccgaactt ctggtcctcg tgcgcggtgg agatgaactc atgggtgtgc gcctcgacgc
      961 cgaccgtcac ccgcaccagc acatcctgca caacgcccgc cgcgcctgag atctcgtcga
     1021 ggcgctcgat ctcggtcatc gaatccaaca cgatgtggcc gacgccggcc ttgaccgcgg
     1081 agcacagttc gtcgacggat ttgttgttgc cgtgcagggc gattcgctcc gcggggaagt
     1141 cggcgtgcaa cgccacggcc aattcgccgc cgctggctac gtccagcgac agaccttctt
     1201 cgttgaccca acgggcgatc tcggtgcaca gaaacgcctt ggccgcatag cggacgtttt
     1261 caccgccgcc gaaggcctcg gcgatctcac ggcagcgcga ccggaagtcg tcctcgtcga
     1321 tgatgaaggc gggcgtgccg aactcgtcag cgatgtcggt gaccttcaca ccggcgatcg
     1381 acaccacacc gtcgtcggcg cgaacggtat tgcggggcca cacattcggt gcgagtacca
     1441 gcagctgttc ggacgactgc ggccgcggag gggcaccccc gtggtgaatc tcctcggcgt
     1501 gccgcggtcc ggcgggatgg gcgttcacat tcgctccggt gcggtgacgc cgagaatccg
     1561 gaggccgttc tcgatgacct ggcgggtggc ctggcacagc gcgaggcgtg cggtgtgcag
     1621 ctcgttgggc tcttcgtcgc cctgaggcaa cacgcggcag gagtcgtaga accggtggta
     1681 g
//
LOCUS       DASYLX010000531         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1562540,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000531 DASYLX010000000
VERSION     DASYLX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562540"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VGP27_12885"
     CDS             complement(<1..1073)
                     /locus_tag="VGP27_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7852229.1"
                     /translation="MTLLKEPDFGTPAKDGPASVSLEVDGLRVAVAEGTSVMRAAALA
                     GVDVPKLCATDSLEAFGSCRLCLVEIDGRRGTPASCTTPVADGMVVRTQTPKVAKLRE
                     SVMELYISDHPLDCLTCSANGDCELQDMAGAVGLREVRYGYDGENHLDAVVDDSNPYF
                     DFDPSKCIACSRCVRACDEIQGTFALTIEGRGFESKVSAGAGESFMDSECVSCGACVQ
                     ACPTATLQEKSVIQLGMPSRSVITTCAYCGVGCSFKAELRGDELVRMVPHKDGGANEG
                     HSCVKGRFAFGYATHPDRVLKPMVREKITDPWREVEWDEAIETVARRMLDIKRRHGAG
                     AIGGITSSRCTNEEVYVVQKMVRA"
     gene            complement(1070..2671)
                     /locus_tag="VGP27_12890"
     CDS             complement(1070..2671)
                     /locus_tag="VGP27_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV7852230.1"
                     /translation="MSAAPVTVYVPRDSAATSVGADQVADALLRAAARVGRPVEVVRN
                     GSRGMLWLEPLVEVVTPDGRIGYGPVTPGAVDNLVAAGMFDGAEHPSRVGVVEDLPWL
                     ATQDRVTFARVGVTDPLSPNDYLNHGGIAGLRRALAMSPADVVTEVTDSGLRGRGGAG
                     FPAGIKWKTVLECVDELKFVCCNADEGDSGTFADRMVMEGDPFMLIEGMTVAAYAVGA
                     SEGYIYIRSEYPDAVAAMRSAIEIARGQGWLGANVLGSSLNFDLHVRVGGGAYICGEE
                     TSMLESLEGKRGMVRAKPPIPAINGLFGKPTVVNNVLTLASVPTILADGAQAYEELGV
                     GRSRGTQLFQLGGNIRHGGIVELAFGVTLGELVDGYGGGTRSGRPVRAVQVGGPLGAY
                     LPTSKFELPMDYEAFVAAGAMVGHGGIVVFDDTVDMAAQARFAMEFCAAESCGKCTPC
                     RVGSVRGVEVIDRITAGEHPDENLALLEDLCDVMTDGSLCAMGGLTPMPVRSAITEFP
                     DDFLAREPISPGMQLPLSVRTKGTP"
     gene            complement(2668..>3024)
                     /locus_tag="VGP27_12895"
     CDS             complement(2668..>3024)
                     /locus_tag="VGP27_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV7852231.1"
                     /translation="PPEAIPVLADELNLSRADVHGVVSFYHDFRTEPAGRATVRVCRA
                     EACQAVGAGLLVAHLRDRYGVSLGETSRDGSLTAEQVFCLGNCALGPAAQVNGRLHGR
                     LDEDRLSSIIEKAISQ"
BASE COUNT          505 a         1081 c          959 g          479 t
ORIGIN      
        1 gcgcgaacca tcttctggac gacgtagacc tcttcgttcg tgcaccgcga cgacgtgatc
       61 ccgccgatcg cccccgcgcc gtgccggcgc ttgatgtcga gcatccgacg ggcgacggtc
      121 tcgatcgcct cgtcccactc gacctcacgc cacgggtcgg tgatcttctc gcgaaccatc
      181 ggtttcagca cccggtccgg atgggtcgcg tagccgaagg cgaaccgacc tttcacgcac
      241 gagtgcccct cgttggcgcc gccgtccttg tggggcacca tgcggaccag ttcgtcgccg
      301 cgcaactccg ccttgaacga gcagccgacg ccgcagtagg cgcacgtggt gatcaccgac
      361 cgcgacggca tccccagctg gatcaccgac ttctcctgca gggtggccgt cgggcacgcc
      421 tgcacgcagg cgccgcacga aacgcactcc gagtccatga acgactcgcc ggcgcctgcc
      481 gacaccttcg attcgaagcc gcggccctcg atggtcagcg cgaacgtgcc ctggatctcg
      541 tcacaggccc gcacgcagcg cgagcaggca atgcatttgg acgggtcgaa gtcgaagtac
      601 gggttgctgt cgtcgacgac ggcgtccagg tggttctccc cgtcgtagcc gtacctgacc
      661 tcacgcagtc cgaccgcgcc cgccatgtcc tgcaattcgc aatcaccgtt ggccgagcag
      721 gtcagacaat ccagcggatg gtcggagatg tagagctcca tgacgctctc gcggagcttg
      781 gcgaccttcg gcgtctgggt ccggaccacc atgccgtcgg ccaccggcgt cgtgcacgag
      841 gcgggagttc cacggcggcc gtctatttcg acgagacaga gccggcagga cccgaatgct
      901 tcgaggctgt cggtggcgca caacttgggc acatcgacac cggccagggc ggccgcccgc
      961 atgacggacg tgccttctgc gacggcgacg cgcagcccgt ccacctcgag cgagacagac
     1021 gcgggaccgt ccttcgccgg agtgccgaag tcgggttcct tgagcagcgt catggtgtac
     1081 ctttcgtcct cacgctgagg ggcagttgca tgcccggcga aatcggttcg cgggcaagga
     1141 agtcgtcggg aaactccgtg atcgcgctgc ggaccggcat cggggtgagg ccgcccatgg
     1201 cgcacaacga gccatcggtc atgacgtcgc agaggtcttc gagcagggcg agattctcgt
     1261 cggggtgttc acccgccgtg atccggtcga tcacctcgac gccgcgcacc gagcccaccc
     1321 gacacggtgt gcacttgccg cacgactccg cggcgcagaa ctccatggcg aatctggcct
     1381 gggccgccat gtcgacggtg tcgtcgaaca ccacgatgcc gccgtggccg accattgcac
     1441 ccgccgcgac gaacgcctcg tagtccatcg gaagttcgaa cttggacgtc ggcaggtacg
     1501 cgccgagcgg accaccgacc tggaccgcac gcaccggacg tccggaacgc gtgccacctc
     1561 cgtagccgtc aacgagctcg cccagcgtca ccccgaaggc caattccacg atgccgccgt
     1621 ggcggatgtt cccgccgagc tggaagagct gggtaccccg cgaccgcccc acgccgagtt
     1681 cctcatacgc ctgcgcaccg tcggcaagga tggtcggcac gctggccagc gtgagcacgt
     1741 tgttcaccac cgttggcttg ccgaacagac cgttgatggc cgggatcggc ggcttcgcgc
     1801 ggaccatgcc gcgcttgccc tcgagactct ccagcatgga ggtctcctcg ccgcagatgt
     1861 aggcgccgcc tcccacccgg acatgcaggt cgaagttcag cgacgagccg agcacgttgg
     1921 ccccgagcca cccttggccc ctcgcgattt cgatggccga gcgcatcgcg gccaccgcgt
     1981 cgggatactc cgaccggatg tagatgtacc cctcgctcgc gcccacggcg tatgccgcga
     2041 ccgtcatgcc ctcgatgagc atgaatgggt ccccctccat gaccatccgg tcggcgaacg
     2101 tgccgctgtc accttcgtcg gcgttgcagc agacgaactt cagttcgtcg acgcattcga
     2161 gaacggtttt ccatttgatg ccggctggga aaccggcgcc gccgcgtccg cgcagtccgg
     2221 agtcggtgac ctccgtgacc acgtcggccg gggacatcgc caatgcccga cgtagaccgg
     2281 cgatgccacc gtggttcaga tagtcgttag gggacaacgg atcggtgacg cccaccctgg
     2341 cgaacgtgac ccggtcctgc gtcgccagcc agggtagatc ctcgaccaca cccacccgcg
     2401 atgggtgctc agcgccatcg aacatgccgg cggccacgag gttgtcgacg gcgccggggg
     2461 tgacgggccc gtagccgata cgtccgtcgg gcgtgaccac ctcgaccagt ggttccagcc
     2521 acagcatccc gcgagacccg ttgcgaacca cctcgactgg ccgtcctacc cgagcggcag
     2581 cgcgcagcag cgcgtccgcg acctggtccg cgccgaccga tgtcgcggcg gaatcccttg
     2641 gcacgtagac ggtcaccggt gccgcgctca ttgcgagatc gccttctcga taatcgacga
     2701 cagccgatcc tcgtccagcc ggccgtgtag ccggccgttc acctgcgcgg ccggtccgag
     2761 cgcgcagttg cccaggcaga aaacctgttc ggcggtcagc gatccgtccc ggctcgtctc
     2821 ccccagcgac acgccatagc gatcccgcag gtgagccacc agtagtccgg cgccgacggc
     2881 ctgacacgcc tcggcgcggc agacgcggac cgtcgcgcgc ccggccggct cggtacggaa
     2941 atcgtgatag aagctgacga ctccgtgtac gtcggcccga gaaaggttga gctcatccgc
     3001 caacacgggg atggcctcag gcgg
//
LOCUS       DASYLX010000532         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2329951,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000532 DASYLX010000000
VERSION     DASYLX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329951"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VGP27_12900"
     CDS             complement(<1..1065)
                     /locus_tag="VGP27_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7852232.1"
                     /translation="MDVPGRPLPEVNAQNEFFWTAGADGVLRIQECMDCSALIHPPRP
                     VCRYCRGRDMGVRDVSGEATLSAFTVNHRFGFPDLPPPYVVAQVAIVEDPRVRLTTNV
                     VDCEPDELEIGQMLEVQFDKAEDKFGTVWLPVFKPTVEKKTGPQAGDEIAPQDFGKHV
                     SPMLTKEKFEDAAAITGIGMSKIGRRQMVPPLSLTVDACQQAVADAGLTFDDIDGLST
                     YPGLDIAGMGEGGVSALEGALGLRPVWINGGMDTFGPGGSLIAAVMAIATGMARHVLC
                     FRTLWEATFQQLMKEGKMSPPGGARTSSWQMPFGATSAAHTLALNAQRHFQRYGTTRE
                     TLGYIALNQRANAALNPTAIY"
     gene            complement(1067..1309)
                     /locus_tag="VGP27_12905"
     CDS             complement(1067..1309)
                     /locus_tag="VGP27_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7852233.1"
                     /translation="MRIRLDRTMCDGFGQCAKHAPEYFSLDDWGYASLEGNGTIPEAD
                     RDAVMRALLDCPVHAIIEMAEARQQSSGAAEQDARS"
     gene            complement(1306..2595)
                     /locus_tag="VGP27_12910"
     CDS             complement(1306..2595)
                     /locus_tag="VGP27_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEV7852234.1"
                     /translation="MTATTDLAVTVWPGLEPRLLRQGGEDYAEYRDSDGYRPLDDPDG
                     LLSQIDLSGILGRGGAAFPLAVKLKTVRANGVDAGGAVVVANGEEGEPASIKDRWLLR
                     HRPHLVLDGLRLAATMVSAACAHLYVSDRCAAESVQSALAEIDRDVLSGLSISVMTVD
                     PGYVAGEETAAVRALNGGPAKPTDKPPRPFEEGVGRLPTMVSNVETLALIPFIARHGA
                     EAFRAQGTSASPGTFLATITGAGKPPALYELPHGLAFTDLLESHGVPTDQVHGVLMGG
                     YFAGLLNRDVLDITLDHESLRRLDSGMGCGAITVLTEDCPVAVAASVMAYFDRENAGQ
                     CGSCFNGTAAMSAVTEALRDGVATNEDLARLERWSVVLRGRGACATLDAATNVASSLL
                     SAFPQVVDRHLNNDCDSCRTGAFKALRPYEIEAVMPT"
     gene            2629..>3028
                     /locus_tag="VGP27_12915"
     CDS             2629..>3028
                     /locus_tag="VGP27_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852235.1"
                     /translation="MRTAVVRVQVDPTGHLAPEQLTEGMTALRALLGAAGIELLDNDI
                     AAMPAARREVQMLMVGGDPEKLKRDAVALCASAFSTEPAAGVVTYVSRGTDDDAHGVL
                     AGFGLAGEISRTPGDDGLDIVHVTLRKADLD"
BASE COUNT          498 a         1053 c          979 g          498 t
ORIGIN      
        1 gtagatcgcc gtcgggttca gcgccgcatt cgccctctga ttcaacgcga tatagcccag
       61 cgtctccctt gtggtgccat accgctggaa gtggcgttgc gcattcaacg cgagtgtgtg
      121 cgcggccgac gtcgcgccga acggcatctg ccaactcgag gtgcgggcgc ccccgggcgg
      181 cgacatcttg ccttccttca tcaactgttg gaaggtcgcc tcccacagcg tgcggaagca
      241 cagcacgtgg cgggccatgc cggtggcaat ggccatcacc gccgcgatca gcgatccgcc
      301 gggaccaaag gtgtccatcc cgccgttgat ccacaccgga cgcagaccca gcgcaccctc
      361 cagcgcgctg accccacctt cgcccatgcc ggcgatgtca agccccggat acgttgagag
      421 cccgtcgatg tcgtcgaatg tcagacccgc gtcggccacc gcctgctggc atgcgtcgac
      481 ggtcaacgac agcggcggca ccatctggcg cctgccgatc ttcgacatgc cgatcccggt
      541 gatcgccgcc gcgtcctcga acttctcctt ggtgagcatc ggcgagacgt gcttgccgaa
      601 gtcttgcggt gcgatctcgt cgccggcctg cggcccggtc ttcttctcga cggtgggctt
      661 gaacaccggc aaccacacgg tgccgaactt gtcctcggcc ttgtcgaact gaacctcgag
      721 catttgaccg atctcgagct catcgggttc gcagtcaacg acgttggtgg tcaaccgaac
      781 ccgtgggtcc tcgacgatcg cgacctgtgc caccacgtac ggcggtggca gatcgggaaa
      841 accgaaacga tggttgacgg tgaaagccga gagggtggct tcgccggaca cgtcgcgcac
      901 gcccatgtct cggcctcggc aatatcggca caccggccgc ggcggatgaa tcagcgcact
      961 gcagtccatg cactcctgaa tgcgcagcac accgtcggca cctgcggtcc agaagaactc
     1021 gttctgtgcg ttgacttctg gcagcggacg accgggaacg tccacctcag ctcctcgcgt
     1081 cctgttcggc cgccccggac gactgctggc gggcttcggc catctcgatg atggcgtgca
     1141 ccgggcagtc cagaagggcc cgcatcaccg cgtcgcgatc ggcctccgga atggtgccgt
     1201 tgccttcgag tgaggcgtat ccccagtcgt ccagggaaaa gtattccggt gcgtgcttgg
     1261 cgcactggcc gaaaccgtcg cacatggtcc ggtcgagacg aatcctcatg tcggcatcac
     1321 cgcctcgatc tcgtagggcc gcagtgcctt gaaggcgcct gtgcggcatg aatcgcagtc
     1381 gttgttcaga tgccggtcga ccacttgcgg gaacgccgac agcaggctgg acgccacgtt
     1441 cgtcgcggca tcgagtgtcg cgcaggcgcc gcggccgcgc agtaccaccg accaccgctc
     1501 caaccgcgcc agatcctcgt tcgtcgccac cccgtcgcgc agcgcttcgg tcactgccga
     1561 catcgcggcg gtgccgttga aacacgatcc gcactggccc gcgttctccc ggtcgaagta
     1621 cgccatcacc gacgccgcca ccgcgaccgg gcagtcctcg gtgaggacgg tgatcgcgcc
     1681 gcagcccatt ccgctgtcga ggcgacgcaa cgactcgtgg tcgagcgtga tgtcgaggac
     1741 atcgcggttc agcagcccgg cgaagtagcc gcccatcaga acgccgtgca cctgatctgt
     1801 cggcacgcca tgggattcga gcaggtcggt gaacgccaac ccgtgcggaa gctcgtacag
     1861 cgcaggcggt ttgcccgcac cggtgatcgt cgcaaggaat gttcccggcg acgccgacgt
     1921 cccctgcgcg cggaatgcct cggcaccgtg ccgggcgatg aacgggatga gtgcgagcgt
     1981 ctcgacattg ctgaccatcg tcggaaggcg gccgacgcct tcttcgaacg ggcgcggcgg
     2041 cttgtcggtc ggcttggccg ggccgccgtt gagtgcacgc acggcggccg tctcctcgcc
     2101 tgcgacgtag ccggggtcca cggtcatcac cgagatggac agaccgctga gcacgtcgcg
     2161 atcgatctcg gcgagcgcgg actgcaccga ttccgcggca catcggtcgg agacatagag
     2221 gtgggcgcac gcagcggaca ccatcgtcgc cgccagccgt agcccgtcga gcaccaggtg
     2281 cgggcggtgc cgcagcagcc accgatcctt gatcgacgcg ggttcaccct cctcaccgtt
     2341 ggcgaccacc accgctccgc cggcatcgac gccgttggcg cggaccgtct tcagcttgac
     2401 cgccagcggg aaggccgcac cgccgcggcc gagaatgccg gacagatcga tctgactcaa
     2461 caggccgtcc ggatcgtcga gcggccggta accgtccgag tcacggtatt cggcgtaatc
     2521 ctcgccgccc tgtcgcaaca gccgtggctc gaggcccggc cacaccgtga cggcgagatc
     2581 ggttgtcgct gtcacggcat cctcctgacc gcggataggg ttgcggacat gaggaccgcg
     2641 gtggtgcgtg tccaggtgga ccccacgggc cacctcgcgc cggaacaact aacggaaggc
     2701 atgacggccc tccgcgctct cctcggcgcc gcgggtatcg agctgctcga caacgacatc
     2761 gccgcgatgc ctgcggcccg ccgggaagtg cagatgctga tggtgggcgg cgaccccgag
     2821 aagctgaagc gggacgcggt cgccttgtgc gcgagcgcgt tttctaccga gcccgctgcc
     2881 ggtgtggtga cctacgtgag ccggggtacc gacgacgacg ctcacggtgt gctcgccggc
     2941 ttcggcctgg ccggcgagat cagccgcaca ccgggggacg acggcctcga catcgtccat
     3001 gtcacgctgc gcaaggccga cctcgacc
//
LOCUS       DASYLX010000533         6605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_354183,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000533 DASYLX010000000
VERSION     DASYLX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354183"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1177)
                     /locus_tag="VGP27_12920"
     CDS             complement(59..1177)
                     /locus_tag="VGP27_12920"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852236.1"
                     /translation="MITAETVNRIVGFRGNGMPVISLYAVVPYEPQDRGTVVRSKVDS
                     LLHEIRPMAQDHRQDHDVMLSVRADIERFTKAVEEHDGWSPGSLALFSCSGRDFFEEV
                     QLPRSVHDRIVVDETPWVRPMLAVLDEYHRCCVAVVDRTTARIWELYQEEMSEIDTQR
                     VGADPGRNQDKAEELTKRHFRDVVTMLDRLHRDGAFELLIVGGHHAEVPRFLDYLTHE
                     LRPTLAGTFTVDDDARTAFGDVKQQAAATLDRYERDEEERMVAETVEKSAAGGWAVLG
                     LPECLWAGSVAAVDALLVQDGAVAPGVICDNDRWLALSGDTCPLCGRPVRRAPDIVDE
                     LVQVVMNESGSIEHVSADTVLKQHLTAASLRFPLPPKP"
     gene            complement(1174..1362)
                     /locus_tag="VGP27_12925"
     CDS             complement(1174..1362)
                     /locus_tag="VGP27_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852237.1"
                     /translation="MTTQAGLEKKERDFLASCVADAYTMLDIVPGVDPGGPALVWLAD
                     RFVMLRRGDCPPARAMTL"
     gene            complement(2471..2917)
                     /locus_tag="VGP27_12930"
     CDS             complement(2471..2917)
                     /locus_tag="VGP27_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852238.1"
                     /translation="MLYWIAILVSNRLGTSSGDFLADSLGLGFVPSVVVLPTALLALL
                     ALHYWTRVNGMLLFWIAFVLTRPLGANLGNVLTKEHGRGGLGWGTTWTSAALLAALIG
                     LVAYETVQVRRQPLGFLPVPLHRRTGERQQPNGALVSVRSQTTSVD"
     gene            complement(2972..3322)
                     /locus_tag="VGP27_12935"
     CDS             complement(2972..3322)
                     /locus_tag="VGP27_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852239.1"
                     /translation="MMAALARAADTAAISLFRRFIPMVIPTFQYSRSGCPITLPMEDC
                     EDAEMACGTFQERCNARGESRLPSHPAIFWTAVAMISTAGTTLSDLMNRTGGIGYSVG
                     SLVLSTGLVIVFVA"
     gene            3694..4386
                     /locus_tag="VGP27_12940"
     CDS             3694..4386
                     /locus_tag="VGP27_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7852240.1"
                     /translation="MRVLIVEDEADLTDTLAVGLRADGFAVDVARDGAAGLARAQATS
                     YEAIVLDIMLPTLSGYEVVHRLRAGGIWTPVVMLTAKDGEYDEADALDLGADDYITKP
                     FSYVVLLAHLRSVIRRGAQKRPAELAVGDLALDLATRKCRRDCNEIELTRREFAVLEY
                     LMHRHDEVVPKSDILANVWDVFYDGDPNIVEVYVGYLRRKIDAPFARHSIETIRGAGY
                     RLRDDARRPARA"
     gene            4383..5891
                     /locus_tag="VGP27_12945"
     CDS             4383..5891
                     /locus_tag="VGP27_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7852241.1"
                     /translation="MSDRELPRNRSPTPQWWYRRSLRARLTVAAVAVIAVGMGAAAAL
                     LLWRMHAVLAGNLDTSLTQQIHAAAEDVAAGSAPPRVWKTAGASSALQVVDGAGRVTA
                     SAGDVDGASRMFFTTPAVGDPAIVTVSNPGLEAPYRVAAMSVTTRTGPVKLYIGSPTT
                     TLATMRAALGSALIVGIPALVALLALVSWWLLGRALRPVETMRRQAAAIPGDDLRRRL
                     DVSAAKDELGRLSKTFNELLERIAVASDRQRRFVDDAAHELRSPLAALQTRLEIGLRH
                     PTAGIVDSEERAYGQRQALKEITRLVDLVNDLLSLARADAGRRPRSRPIDLDDLVWRA
                     ATDAREHGPPKIDTTGIAPVRVVGDTAGLRRVVQNLLDNARRHANDTVTVQLRLNFEP
                     PSSGGDSAAVPTATLIVADDGPGIPVADRQRVFDRFVRLDEGRPRDSGGTGLGLAIVS
                     AIVAEHGGRVWIDGGEAGTAFVVQLPAVPSELGGATKAAAPQQNRPESTDPM"
     gene            6138..>6605
                     /locus_tag="VGP27_12950"
     CDS             6138..>6605
                     /locus_tag="VGP27_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852242.1"
                     /translation="METPVPENNPIGDIPDTQVYVPFTAADGSFTLSVPEGWAQTADG
                     SAVAFTDKLNTVRIDARPAVSAPSPESVKRGELSSVKTANGGFTVEAVKRRSGDAVLA
                     TYQAASAPNPVTGKAGIDAVERYSFWRAGQEVILTLSGPVGADNVDPWRTITDS"
BASE COUNT         1186 a         2097 c         2177 g         1145 t
ORIGIN      
        1 cccggccagc gcctcgtcca gtccggcctg gtctcgaact tcgcgacgac gaagatcatc
       61 acggtttggg cggaagcgga aatcgcagcg atgcggctgt caggtgctgc ttgaggacgg
      121 tgtcggcgga gacgtgttcg atcgacccgc tctcgttcat caccacttgg accagctcgt
      181 ccacgatgtc tggggcacgg cgcaccgggc gtccacatag cgggcacgta tcgccggaaa
      241 gggccaacca gcgatcgttg tcgcatatca cgcccggtgc taccgctcca tcctggacca
      301 ggagcgcgtc caccgctgcg acgcttccgg cccacaggca ttcgggaaga ccgaggacgg
      361 cccatccgcc cgcagccgac ttctccaccg tttcggcgac catccgctcc tcctcgtcgc
      421 gctcatagcg gtcgagtgtc gcggcggcct gctgcttgac atcgccgaag gcggtgcgcg
      481 catcgtcgtc caccgtgaag gtgccggcga gggtcggccg cagttcgtgg gtgaggtaat
      541 caaggaagcg cggtacctcc gcgtggtggc cgccgacgat caacagctcg aatgcgccgt
      601 cgcggtgcag cctgtcgagc atggtcacca cgtcgcggaa gtgacgcttg gtcagttcct
      661 cggccttgtc ttgattgcgg ccggggtcgg cgccaacccg ctgtgtgtcg atctcagaca
      721 tctcttcctg gtagagctcc caaatccggg cggtcgttcg gtcgacgacc gcgacgcaac
      781 agcggtgata ctcgtcgagc accgccaaca tcggccggac ccaaggtgtt tcatcgacca
      841 caatccggtc atgcacgctg cggggaagct gaacttcctc gaagaagtca cgaccgctgc
      901 acgagaacag cgcaagcgaa ccgggcgacc aaccgtcgtg ctcctcgacc gccttggtga
      961 accgctcgat atcggcacgg acggacagca tcacgtcgtg gtcttgccgg tggtcctgcg
     1021 ccatcggccg gatttcgtgc agcaggctgt ctaccttgct gcgaacgact gtgccacggt
     1081 cctggggctc gtacggcacg acggcataca acgagatgac cggcatgccg ttcccgcgaa
     1141 agccaactat ccggttgact gtctccgctg tgatcacagc gtcatcgcac gtgcaggcgg
     1201 gcaatcccca cggcgcaaca tcacgaaccg atcggcaagc cagaccaggg caggaccccc
     1261 ggggtccaca ccggggacga tgtccagcat cgtgtatgca tcggccacgc acgacgccag
     1321 gaagtcgcgt tccttcttct caaggccagc ttgtgtcgtc atatcgggct ccttcctaag
     1381 acggtggtgg cggcggaaac atccctcaac ccgtaccttg cccaacgacg cagccgcgta
     1441 cctcagcccc ccgacaccag gtaatggggg tcgagttgac cctgcaagac gagcacgcag
     1501 catcatccct ggttcgcccg ctgctcaggt gtgtctgacg attcgataag gcgtcgacga
     1561 acagggcccc ggttggcgcc gtaacccgtc taggaggtgc tcttcgacgg aggcctccac
     1621 ggccctaggt tgtggtgggg gcggcgtgag tgcggttgga gcccgaccaa gcaacagcag
     1681 gcaaaggggg ttcggagtgc tcgggggccg cctcttcgca tcgatgacgg ccaccacggc
     1741 gtcagccaga tcccgatttc gctttggcgg ccgccttgcc ctctggactc tgagcggagt
     1801 aaccgggcgc ttttgcagac gccggcgacg cattgcagtt cagtgagaca agtatctggt
     1861 gcctcacgga tcccttcgag ttcgctggcg gtccaacgtc acgtttttgc gcggaggcga
     1921 ccaggcagtc gggccacgcc ttgcggtctc ccaccgttgt cgacacgact ggctcgaacc
     1981 ccgcagcact caacgccgaa gaggcatcac tgtacttctg gcccgtaacg tccggcgacg
     2041 aggccgcggc agcctgtggc aactggcccg ccactaagcc gacaaaccat aggccagcga
     2101 acattgcgac taggcgcgac gtcgtcgatc cgctcatgct aatgcggccc aggtacggca
     2161 gccgggattt tcggctgcgg gctggacgga gtccacgtcg ccgtcgcgcc ggtttgcatc
     2221 ccactgagga cgggttgcgt gtagacggtg ttggtcgccg cgctacccga gccggcaacg
     2281 acgtgcggtc cgggcccgat gtcgaccctg ccgctgtaga cggcccctag tccaccgaga
     2341 gcgaccactg cgccaatgcc gaggagggct agcggcttta ccggcctccg tttcagtgtc
     2401 gaattaggtt tcatgcgttc acgattgcgc gcggccgctg aattattgct gaggccagct
     2461 gagaggacga ctagtcaacg ctcgtcgttt gtgaacgcac ggagaccaat gcaccgttgg
     2521 gctgttggcg ttcgcctgtg cggcggtgaa gcggcacggg aaggaagccg agcggctgcc
     2581 gtcggacttg gacggtctcg tacgccacca agccgatgag agcagccagc aaagcggccg
     2641 aggtccaggt ggtaccccac ccgagcccgc ctcgcccgtg ctccttggtc agcacattgc
     2701 ccaagttggc ccccagcgga cgagttagca cgaatgcgat ccagaagagc agcatgccgt
     2761 tgactcgcgt ccagtagtga agcgcgagga gtgccaacag agccgtcggg agcacaacca
     2821 cgctcggcac gaagcccaat cccaaactgt cagcgaggaa gtcaccgctt gaggtgccca
     2881 aacggttaga caccaggatc gcgatccaat agagcatttc accacggaat gtcgcgatgt
     2941 tctcgacgtc gagagtttga ccgcttctcc atcacgcgac gaagacgatg acaaggcccg
     3001 tggaaagcac aagggagcca acgctgtagc cgataccgcc ggtgcggttc atgagatccg
     3061 acagcgttgt tccagcagtg ctgatcatgg cgacggccgt ccaaaagatc gctggatgac
     3121 tcggcagcct cgattcgcct cgggcgttgc agcgttcctg aaaagtgcca caggccattt
     3181 cagcgtcttc acagtcctcc atcggcaacg tgatgggaca gccagatcgg ctgtattgaa
     3241 aggtaggaat caccatggga atgaaccgac ggaacaaact aatggctgcg gtgtcggccg
     3301 ctctggcgag cgctgccatc attggcggtg ccgcgattgc cagtgcgaca ccgactccgg
     3361 cccctccgcc gagcccgccc gcggcgacgg acagcccgga gcccggtgat acgccggatg
     3421 ttcctggcga ggtcgatacc ccggaacccg gtgatacgcc ggacgctccg ggcgcagtcg
     3481 acactcccga gccgggcgat acgccggacg ctcccgtcac cccgccgccg ggaggctgac
     3541 cagctctccg tgggcgcatg cgattgtgcg cccacggaaa gcgcggtgtg accggcggcg
     3601 ccgggtcggc ggccttcagc gtggcttcag acaggtgttg gcaccgtggt ggttggagcg
     3661 gtgcgtgctg tcaccgggaa ggcgggttca gcgatgcgtg tgttgatcgt cgaggacgag
     3721 gctgatctga ccgatacgct ggccgtcggg ttgcgggccg atggcttcgc cgtcgatgtc
     3781 gcccgcgacg gcgccgccgg gctggcacga gcccaggcaa cctcctacga agcgatcgtc
     3841 ctggacatca tgttgcccac gctgtccggg tacgaggtgg tgcaccggct gcgggcagga
     3901 gggatctgga cgccggtcgt gatgctgacc gcaaaggacg gcgagtacga cgaggcggac
     3961 gcgttggacc ttggtgccga cgactacatc acgaagccgt tctcgtacgt cgtgctgctt
     4021 gcccacctgc gttctgtcat ccgccgcggg gcgcagaaac ggccggcgga gctggccgtc
     4081 ggggatctcg cgctcgacct ggcgacgcgg aaatgcagac gagactgcaa tgaaatcgag
     4141 ctgacgcgaa gggaattcgc tgtccttgag tacctgatgc accgacatga cgaggtggtg
     4201 ccgaagtccg acatcctggc caacgtctgg gacgtcttct acgatggcga tcccaacatc
     4261 gtcgaggtct atgtcggata cctgcgacgc aagatcgatg cgccatttgc ccggcacagc
     4321 atcgagacca tccgcggtgc gggataccga cttcgtgacg acgcgcgacg gccggcccgg
     4381 gcatgagtga ccgtgagttg ccccgcaacc gctcaccgac cccgcagtgg tggtaccgcc
     4441 ggtcgttgcg ggcgcgattg acagtagcgg cggtggccgt gatcgccgtt ggaatgggcg
     4501 ctgcagcggc gctgctgttg tggcgaatgc acgcggtgct cgcaggaaat ctggacacca
     4561 gcctgacgca acaaattcat gcggcggccg aggatgttgc cgcaggcagc gccccaccgc
     4621 gtgtttggaa gacggccggt gcttcgtcgg cacttcaggt ggtcgatggg gcaggacggg
     4681 tgactgccag cgcaggcgac gttgacgggg catcgcgaat gttcttcacc acgcccgccg
     4741 tcggcgaccc agcgatcgtc accgtcagta atcctgggct ggaagcgccg taccgggtag
     4801 ctgcgatgtc ggtaactacg cgcaccggtc cggtaaagct ctacatcggt tcacccacta
     4861 ccacgctggc caccatgagg gcagcactcg gaagcgcatt gatcgtcggc atacccgcat
     4921 tggtagcgct attggcattg gtgagttggt ggctgctcgg tagggcattg cgaccggtgg
     4981 agactatgcg gcgccaggcc gctgcaattc ctggtgatga ccttcggcgc cgactcgacg
     5041 tatccgccgc caaggacgag ttggggcggc tttcgaagac cttcaacgag ctgctcgaac
     5101 gcatcgcggt ggcatcggat cggcaacgcc gctttgtgga tgacgccgca catgaattgc
     5161 gtagtccgtt ggcggcacta caaacccgcc tggagatcgg tctgcgccac ccgacggccg
     5221 gcatcgtgga ttcggaggag agggcgtacg gccaacgcca ggcgctgaag gagataaccc
     5281 ggctggttga cctcgtcaac gatctgcttt cgctggcccg cgcggacgcg ggccgccgac
     5341 cgcgaagtcg accgatcgat ctcgacgacc tggtgtggcg ggcggcgaca gatgcgcgtg
     5401 agcatgggcc gccgaagatc gacaccacag gtatcgcgcc cgtacgcgtc gtcggcgata
     5461 cagccgggct gcgccgggtg gtgcagaatc tcctcgacaa tgcacgccgg cacgccaacg
     5521 acaccgtgac cgtccaactg cgcctgaatt tcgagccccc gtcgtccggt ggggatagcg
     5581 cggcggtgcc gacggcgacg ctcattgtcg ccgatgacgg tcccggaatc cccgtggccg
     5641 accggcaacg agtgttcgat cgattcgtac gactcgacga gggtcggccg cgggattccg
     5701 ggggcaccgg cctcgggctc gccatcgtct cggctatcgt tgccgagcac ggtggacggg
     5761 tgtggattga cggtggcgag gccggtaccg catttgtcgt gcagctgccg gccgtcccat
     5821 cagagcttgg tggcgccacg aaagcggctg cgccgcagca gaaccgtcca gagtcaacgg
     5881 atccgatgta atcaccactg aggaggcgaa acggccgtca gggtgttgcg aactcccgcg
     5941 cgggcccgtt tcagcgtcct ttcagccggc acatgacacc gtcttggcat gacggcagcg
     6001 ccggaacgga gggcatcgtg cacatgaggc gcattgcctc ggtgggcgtc ctatgcttgt
     6061 cggtcgcgct cgtactgggt ggatgtgggt cgaatgcgac gacgtcggcc tcgggcacgc
     6121 aaaccagcgt cacttcagtg gagacgccgg ttccagagaa caacccgatc ggagacatcc
     6181 ccgataccca ggtgtacgtg ccgtttacgg cggccgacgg atcattcacg ctgtcggtcc
     6241 ccgagggttg ggcccaaacg gccgacggct ccgcggtggc cttcaccgac aagctcaaca
     6301 cggtgcgcat cgatgcgcgg cctgccgttt cggccccttc gccggagtcg gtgaaacgtg
     6361 gcgagttgtc ttcggtgaag accgccaacg gagggttcac agtcgaggcg gtcaagcgta
     6421 ggtctggtga cgccgtgttg gccacctatc aggctgcgtc ggcgccgaac ccggtgaccg
     6481 gcaaggccgg aatcgacgcc gtagaacgtt actcattctg gcgcgcaggc caggaggtga
     6541 tcctgaccct ctctggaccc gtgggagcgg acaacgtcga tccgtggcgg acgatcaccg
     6601 actcg
//
LOCUS       DASYLX010000534         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2657763,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000534 DASYLX010000000
VERSION     DASYLX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657763"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGP27_12955"
     CDS             complement(<1..1193)
                     /locus_tag="VGP27_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV7852243.1"
                     /translation="MITATDLEVRAGARTLLSTDGTALRVQPGDRIGLVGRNGAGKTT
                     TMRILAGEAEPYAGSIARTGEIGYLPQDPREGNLDTLARDRVLSARGLDTLLTDLEKQ
                     QALMAEVADDATRDRAVRRYGQLEERFAALGGYGAESEASRICASLGLPDRVLTQPLR
                     TLSGGQRRRVELSRILFAASDSGAGSSTTLLLDEPTNHLDADSLGWLREFLRGHTGGL
                     VVISHNVELLADVVNRVWFLDAVRGEVDVYNMSWQKYLDARASDEQRRRRERANAERK
                     ATALRTQAAKMGAKATKAVAAQNMLRRADRMMAALDEERVADKVARIKFPSPASCGKT
                     PLVAKDLTKMYGSLEVFAGVDLAIDRGSRVVVLGLNGAGKTTLLRLLAGVETPDTGGL
                     EPGHGL"
     gene            complement(1214..2044)
                     /locus_tag="VGP27_12960"
     CDS             complement(1214..2044)
                     /locus_tag="VGP27_12960"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7852244.1"
                     /translation="MQSVTEQTDFVLVDRPRPNVALVTLNRPERMNSMAFDVMVPLKA
                     VLEELTHDNSVRAVVLTGAGRGFSSGADHKSAGSVPHIDGLTRPSFALRSMQVLDDVI
                     LALRKMHQPVIAAVNGAAIGGGLCLALAADIRVAGDEAYFRAAGINNGLTASELGLSY
                     LLPRAIGTSRAFELMLTGRDVDAQEAERIGLVSQSVPGDRLLDTCYEMAERIAGFSRP
                     GIELTKRTLWTGLDAASLEGHMQAEGLGQLFVRLLTANFEEAVAARAEKRPAVFTDDK
                     "
     gene            complement(2063..2428)
                     /gene="trxA"
                     /locus_tag="VGP27_12965"
     CDS             complement(2063..2428)
                     /gene="trxA"
                     /locus_tag="VGP27_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7852245.1"
                     /translation="MTTQDLTAAEFNDTINDNDIVLVDFWASWCGPCRAFAPAFAASA
                     DKHPDVVHAKVDTEAEQQLAAAADIRSIPTLMAFKKGKLVFNQAGALPPAALEDLVQQ
                     VKAFDIDAAIAAEGDGEQA"
BASE COUNT          362 a          857 c          853 g          436 t
ORIGIN      
        1 agcccgtgac ccggttccag cccaccggtg tccggtgtct cgacgccggc gagcaggcgc
       61 agcagcgtgg tctttcccgc gccgttgagg ccgagcacca cgacccgaga gccgcggtcg
      121 atggccagat cgacaccggc gaagacctcg agcgagccgt acatcttggt cagatccttg
      181 gccaccagtg gggtcttgcc gcacgacgcc gggctgggga atttgattcg cgcgaccttg
      241 tcggccaccc gctcctcgtc gagcgctgcc atcatccggt cggcccggcg cagcatgttc
      301 tgcgcagcaa cggctttggt ggccttggcg cccatcttgg cggcctgggt gcgcagcgcc
      361 gtggccttgc gttcggcgtt ggcgcgctcg cggcggcgcc gctgctcgtc gctggcgcgc
      421 gcgtcgaggt acttctgcca ggacatgttg tagacgtcca cctcgccgcg cacggcgtcg
      481 aggaaccaca cccggttgac gacgtcggcg agcagctcga cgttgtggct gatcaccacc
      541 aacccgccgg tgtggccgcg caggaactcg cgcagccagc ccaacgagtc ggcatcgagg
      601 tggttggtcg gctcgtcgag cagcaacgtg gtggacgagc cggctccgct gtccgatgcg
      661 gcgaacagga tgcgggagag ttcgacgcgg cggcgctgac cgccggacag ggtgcgcagc
      721 ggttgggtga ggacccgatc gggcaggcca aggcttgcgc agatcctgct ggcctcgctt
      781 tcggcgccgt acccgccgag ggcggcgaac cgctcctcga gctggccgta gcggcgcacc
      841 gcacggtcgc gggtggcgtc gtcggcgacc tctgccatca acgcctgctg cttttccaga
      901 tccgtcagga gggtgtcaag accacgcgcc gagagcaccc ggtcgcgggc gagggtgtca
      961 aggttgcctt ccctgggatc ctgtggcaga taaccgattt cgccggtccg cgcgatgctg
     1021 cccgcatagg gctcagcctc gcctgccagg atgcgcatgg tcgtcgtctt gccggcgccg
     1081 ttcctgccga cgagcccgat gcggtctccg ggctgcaccc gcagcgcggt tccgtccgtg
     1141 gacagcagcg tgcgcgcgcc tgcgcggacc tcgaggtccg ttgcggtgat cacgctactt
     1201 ctccttggtg ctgttacttg tcgtcggtga agaccgcggg ccgcttctcc gcgcgcgcgg
     1261 ctaccgcctc ttcgaagttc gcggtgagca ggcgcacgaa taactgtccc aggccttctg
     1321 cctgcatgtg cccttccagg ctagcggcgt ccagtccagt ccacagtgtg cgcttggtca
     1381 actcgattcc cggccgggag aaccccgcga tccgctcggc catctcgtag caggtgtcga
     1441 gcagccggtc gccaggcacg ctttgcgaca ccagcccgat gcgttcggct tcctgggcgt
     1501 cgacatcgcg gccggtcagc atcagctcga acgcccgtga cgtgccgatc gccctcggca
     1561 gcaggtagct cagcccgagt tcgctcgcgg tcaggccgtt gttgatgccc gccgcgcgga
     1621 agtacgcctc gtcaccggcg accctgatgt cggccgccag cgccaaacac aggcccccgc
     1681 cgatggccgc gccgttcacc gcggcgataa ccggctggtg cattttgcgc agcgcgagga
     1741 tgacgtcgtc gagcacctgc atcgaacgca gcgcgaagct cggccgggtc agcccgtcga
     1801 tgtgcggcac agaacccgcc gacttgtggt cggcgcccga ggagaacccg cgtcccgcgc
     1861 cggtcaacac gaccgcgcgc accgaattgt cgtgggtcag ctcttcgagc acggccttga
     1921 ggggaaccat gacgtcgaac gccatggagt tcatccgctc ggggcgattg agcgtcacca
     1981 acgccacgtt gggacgcggg cggtcgacga gaacgaagtc ggtctgctcg gtcacggact
     2041 gcacgctatc gcgtgccgcg cgttacgcct gctcgccgtc accctccgcg gcgattgcgg
     2101 cgtcgatgtc gaacgccttg acctgctgga caaggtcctc gagggcggca ggcggcagcg
     2161 ctccggcctg attgaagacc agcttgccct tcttgaacgc catcaacgtc ggaatggagc
     2221 ggatgtcggc ggcggcggcg agttgctgct cggcctcggt gtcgaccttc gcgtgcacga
     2281 cgtcgggatg cttgtcggcc gacgcggcga acgccggggc gaacgcgcga cacggcccgc
     2341 accacgacgc ccagaaatcc accagcacga tgtcgttgtc gttgatggtg tcgttgaact
     2401 cggcagcggt gaggtcttgg gtagtcacgc ttctcctaac gccgacgttt tcctgtgtgt
     2461 tcccacaatg tggtcagcgg aacccccggg agtgccgggc tgctgtcc
//
LOCUS       DASYLX010000535         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2218877,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000535 DASYLX010000000
VERSION     DASYLX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218877"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="cobT"
                     /locus_tag="VGP27_12970"
     CDS             complement(<1..923)
                     /gene="cobT"
                     /locus_tag="VGP27_12970"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEV7852246.1"
                     /translation="MTDVPPITAPDEGVAAQARARQNTLTKPPGSLGRLEELSVWVAA
                     CQGVCPPNQFKRARVVVFAGDHGVTAAGVSAFPAEVTAQMVANFEAGGAAINVLAEAA
                     GATVRVADIAVDTDEPHTASIGAYKVRRGSGNIAVEDALSADEVQAAVDAGRRIADEE
                     VDAGADLLIAGDMGIGNTTAATTLIAALTGAEPVVVVGRGTGIDDAGWSRKVAAIRDA
                     LYRARGLAADPLGLLRVCGGADLAAMAGFCAQAAARRTPLLLDGVVVTAAAMVADVLA
                     PGAREWWRAGHRSTEPAHSLALERLELEPIV"
     gene            complement(920..1450)
                     /gene="cobU"
                     /locus_tag="VGP27_12975"
     CDS             complement(920..1450)
                     /gene="cobU"
                     /locus_tag="VGP27_12975"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEV7852247.1"
                     /translation="MRILVLGGIRSGKSEFAESVIADCAGAEASVRYIATGPVADDDD
                     WAARVSAHRERRPRHWSTVESTDLAKQLRTDDSSPTLVDDVAGWLTAAMDRAQAWTGG
                     SVRADTDDLIGAVETHRADLVLVSPEVGLTVVPETVAGRRFADELGVLNQRLAALCDR
                     VVLVVAGQPVTLKGPS"
     gene            complement(1456..2136)
                     /locus_tag="VGP27_12980"
     CDS             complement(1456..2136)
                     /locus_tag="VGP27_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEV7852248.1"
                     /translation="MNLLGRKKDNPPPQDAGATEAAEATADSEADAKASSATAPKGRP
                     TPKRDSSRRRGPIAPAPMTTAEARRRRKELRGPKLSREERREDKIARRAAQSDRREHM
                     MAGDDDYLLPRDKGPVRRFARDIVDSRRVSVLGLFMPAALGLIFIMLAVPQVQYYISP
                     AMLVLMAIMIVDGLLLGRKVNRLADQKYPDHTESGWKLGFYAASRASQLRRMRAPRPQ
                     VNRGDKIA"
BASE COUNT          309 a          815 c          750 g          354 t
ORIGIN      
        1 tcgacgatcg gttcgagttc cagcctctcc agcgccagtg agtgcgccgg ctcggtggac
       61 cgatggcccg cccgccacca ctcacgcgcg cccggcgcca gcacgtcggc gaccatcgcg
      121 gcagccgtca ccaccacccc gtcgagcaac agcggagtcc gacgtgccgc cgcctgcgcg
      181 cagaacccgg ccatcgccgc caggtccgcg ccgccgcaca cccgcagcag cccgagcggg
      241 tcggccgcga ggccgcgggc ccggtacagc gcgtcgcgga tcgccgcgac cttgcgcgac
      301 caacccgcgt cgtcgatccc ggtgccgcgg ccgacaacga cgaccggttc ggcaccggtc
      361 agcgcggcga tcaaagtcgt tgcggcagtg gtgttcccga ttcccatgtc gcctgcgatc
      421 agcaggtcgg cgcccgcatc gacttcctcg tcggcgatcc ggcggcctgc atcgaccgcg
      481 gcctgcacct cgtcggccga cagtgcgtcc tcgacggcga tgttgccgct gcccctgcgg
      541 accttgtatg cgccgatcga cgccgtatgt ggttcgtcgg tgtccaccgc gatatcggcc
      601 acgcgcaccg tcgcgcctgc ggcctcggcg agcacgttga tcgcggcgcc accggcttcg
      661 aagttcgcga ccatctgcgc ggtgacttcc gcggggaacg cagaaacccc cgccgcggtg
      721 acgccgtggt cacctgcgaa caccaccacc cgcgcgcgct tgaactgatt gggtgggcac
      781 acgccctggc acgccgccac ccagaccgac aactcctcga gccgtcccag cgagccaggc
      841 ggtttggtca gcgtgttctg ccgggcccgt gcctgcgccg cgacgccttc gtcgggtgcc
      901 gtgatcggcg ggacatccgt catgacggcc ccttcagcgt gaccggctgt ccggcgacga
      961 ccagcaccac ccggtcgcac agcgccgcga gccgttggtt gagcacgccg agttcgtcgg
     1021 cgaaccgccg tcctgcaacg gtttccggca cgaccgtcag gcccacctcg gggctgacga
     1081 gaaccaggtc cgcacggtgg gtttcgacgg cgccgatgag gtcgtcggtg tcggcgcgca
     1141 ccgacccgcc ggtccacgcc tgcgcgcggt ccatcgccgc ggtcaaccag ccggcgacgt
     1201 cgtcgacgag cgtgggcgag gaatcgtctg tccgtaattg cttggccaaa tcggtacttt
     1261 cgacggtcga ccagtgtcgg gggcggcgct cacggtgagc ggacacgcgc gccgcccagt
     1321 cgtcgtcgtc ggcgacgggt ccggtggcga tataccggac cgacgcctcg gcgcccgcgc
     1381 aatcggcgat gacggattcg gcgaactcgg atttgcccga ccggataccg ccgagcacca
     1441 gaatgcgcac ccgactcagg cgattttgtc gccgcggttg acctgcggcc gtggcgcacg
     1501 catccgacgc agttgcgatg cgcggctggc ggcgtagaac ccgagcttcc aaccgctttc
     1561 ggtgtgatcg gggtacttct ggtcggcgag ccgattcacc ttgcggccca gcaatagccc
     1621 gtcgacgatc atgatggcca tcaggaccag catcgccggt gagatgtagt actgcacctg
     1681 cggaaccgcc agcatgatga agatcaaccc cagcgccgcg ggcatgaaga ggccgagtac
     1741 gctgacgcgg cgcgaatcca cgatgtcgcg ggcgaaccgg cgcaccgggc ccttgtcgcg
     1801 gggcagcagg tagtcgtcgt cgcctgccat catgtgttcg cggcgatcgg actgcgccgc
     1861 gcgccgggcg atcttgtcct cgcgccgttc ctcgcgcgac agcttcgggc cccgcaattc
     1921 cttgcgtctg cgtctggctt ccgcggtggt catcggggcg ggtgcaatgg gaccgcgtcg
     1981 gcgagacgag tcccgcttcg gcgtcggcct gcccttcggg gcggtggcac tcgacgcctt
     2041 ggcgtcggct tcgctgtccg cggtcgcctc ggcggcctca gtggctccgg cgtcttgggg
     2101 aggcgggttg tccttcttgc ggcccagcag gttcacgccc gccagcataa tctgctggca
     2161 tgaccggctc ggacggcctc cgcgtgctca tcgctcccga ctgctacggc gacagcctca
     2221 ccgcggtg
//
LOCUS       DASYLX010000536         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2657772,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000536 DASYLX010000000
VERSION     DASYLX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657772"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGP27_12985"
     CDS             complement(<1..501)
                     /locus_tag="VGP27_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7852249.1"
                     /translation="MGSGYDDAVPPLIHKGAAWAWRLLVIIAFAVAVIWVITRLEVIV
                     VPVALALMLTALMIPAVDFLDRRGAPRGGAVALVLLTGVAIVGGMLTFVVSQFVTGLP
                     GLVEQVTQSIQSTRTWLIEGPAHLSKDQITNAGNSAIEALRNNQTKLTSGALSTAATV
                     TEIITGA"
     gene            560..1636
                     /locus_tag="VGP27_12990"
     CDS             560..1636
                     /locus_tag="VGP27_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEV7852250.1"
                     /translation="MSHDAPANNARSQEGTTRGKYGWLRWVIIGTAVAVLTVEIVLVW
                     DQLAKAFHSLYAAKWLWVLAAVAAAMASMHSFAQIQRTLLKSAGVDVQQWRSEAAFYA
                     GNALSTTMPGGPVLSATFIYRQQRRWGASPVVASWQLVMSGVLQVVGLALLGLGGAFL
                     LGASKNPLSLIFTLGGFIALLLLAQAVASRPDLIDGIAVRLLTWFNQLRGKPVDTGVD
                     MWREHLHQLESVSLSRRDLTVAFSWSLFNWVADVACLAFAAYAAGGRPSLAGLTVAYA
                     AARAVGSIPLMPGGLLVVEAVLVPGLVSSGMTLAAAISAMLIYRLISWIFIAAIGWVV
                     FFFVFRTEKDVDPDVGLDEAEPDA"
BASE COUNT          255 a          545 c          582 g          310 t
ORIGIN      
        1 tgcgcccgtg atgatttcgg tcacggtggc cgcggtcgac agcgcgccgc tggtgagctt
       61 cgtctggttg ttgcgcagtg cttcgatggc cgagttgccc gcgttggtga tctggtcctt
      121 gctcagatgc gcgggcccct cgatcagcca cgttcgcgtg ctctggatgg actgggtgac
      181 ctgttcgacc aggcccggca ggccggtgac gaactggctg acgacgaatg tcagcatgcc
      241 gccgacaatg gcgacgcctg tcagcagcac gagcgcgacc gcgccgcccc ggggagcgcc
      301 gcggcgatcg agaaaatcga ccgccgggat catcagcgcg gtcagcatca gcgccaacgc
      361 gaccggcacc acgatgacct cgagccgcgt gatcacccag atgactgcta ccgcgaacgc
      421 aatgatcaca agaaggcgcc acgcccacgc tgcacccttg tggatcagag gcgggaccgc
      481 gtcgtcgtac ccagatccga tagaggccat gccggtagcg taacggccgg tcggaggccg
      541 ccaccgctac tctttatgcg tgtcccacga cgcgccggcg aataatgcgc gcagccaaga
      601 gggaaccacg cgcggcaagt acgggtggct gcgctgggtg atcatcggca ccgcggttgc
      661 ggtgttgacg gtcgagatcg tgctggtgtg ggatcagctg gcgaaagcct tccacagcct
      721 ttatgcggcc aaatggctct gggttctcgc cgcggtggcc gcggcgatgg cgtcgatgca
      781 cagcttcgcg cagatccagc gcacgttgct gaagtcggcg ggcgtcgacg tgcaacagtg
      841 gcggtcggaa gccgccttct acgcgggcaa cgcgctgtcc accaccatgc cgggcggtcc
      901 ggtgctctcc gccacgttca tctaccggca gcagcggagg tggggagcct cgccggtggt
      961 ggcgtcctgg cagttggtga tgtcgggtgt gctgcaagtc gtcggcctcg ccctgctggg
     1021 tctgggtggt gcgttcctgt tgggggcctc caaaaaccct ctgtcgctca tctttacgct
     1081 cggcggattc atcgccttgc tgctgcttgc acaggcagtc gcatcgcgcc ccgatctgat
     1141 cgacggtatc gcggtcagac tcctgacgtg gttcaaccag ctgcgcggca agccggtcga
     1201 caccggcgtg gacatgtggc gagagcacct tcaccaactg gagtcggtca gcctcagccg
     1261 gcgcgacctc accgtcgcgt tcagctggtc gctgttcaac tgggtcgccg acgtggcgtg
     1321 tctggcattc gccgcctacg ccgcaggcgg caggccgtcg ctggccgggc tgacggttgc
     1381 gtacgccgct gcgcgggcgg tcggctcgat cccgctgatg ccgggggggc tgctggtcgt
     1441 cgaggcggtg ctggtgcccg gcctggtgtc gagcggcatg acgctggccg ccgcgatctc
     1501 tgcaatgctg atctatcggc tgatcagctg gatcttcatt gcggcgatcg gctgggtggt
     1561 gttcttcttc gtcttccgca ccgaaaagga cgtcgacccc gacgtaggcc tcgacgaggc
     1621 cgaacccgac gcttaggagc acagcttgcc gatcggaaca cgcaccgccg ccgcggtcct
     1681 cggggcgatc ac
//
LOCUS       DASYLX010000537         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3427700,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000537 DASYLX010000000
VERSION     DASYLX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427700"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VGP27_12995"
     CDS             complement(<1..1728)
                     /locus_tag="VGP27_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7852251.1"
                     /translation="MAGLTDGLVLTDEFRQALELLAGGRHVFLTGKAGTGKSTLIRHF
                     MAETDRKVVVVAPTGIAALNVDGYTIHRLFGFRTTTTLDGVRSGEYRPGRFAKTLASL
                     QTLIIDEASMVRADVFDMVASALSRFGPDPGTPFGGVQLVLVGDLYQLPPVVTEDEAH
                     FFSTTYETPYFFSAHSFRRDDFPTVSLTTVFRQLGDDRMTAILNEIREGVLLGHAQEQ
                     LNARADADFVPPDDEFWLTLAPTNRLVTARNRQQLERLPGDELVHHARVYGDLSLFDP
                     PVEETLRFKVGAQVMMLNNDQSDRWVNGTIGRVMGVDYDRYGAVVEVEFADGSCADVT
                     PYSWEATRPVVGGGSLGREVIGSYTQLPFKLAWAITIHKSQGQTLERLVVDLTGGMFS
                     TGQLYVALSRCTSLAGLVLKRPVLPKDLKTDRRIARFLRTSVVDSGERRYCAIGMLTV
                     GDEGRMSRPRPVELAVAFDDGTAVTTLINPQRDLADARKAFGISVSDVLLAPTLREAW
                     AVIAPMLAGCTPVGAGVDETLGLIDFELKRLGYVTAMPLGVELRGARVSGRTALERAQ
                     SALEAHSELR"
BASE COUNT          302 a          613 c          555 g          282 t
ORIGIN      
        1 cctcagctcg ctatgcgcct ccagcgcaga ctgcgcccgc tccagcgctg tccggcccga
       61 cacccgcgcg ccgcgcagct cgacgccgag cggcatcgct gtcacatagc cgagcctctt
      121 gagctcgaaa tcgatcaggc ccagggtctc gtccactccg gcgcctaccg gtgtgcatcc
      181 cgcgagcatc ggcgctatca ccgcccacgc ctcccgcaga gtcggcgcca gcagcacgtc
      241 ggagacgctg ataccgaacg ccttccgcgc atccgcgaga tcccgctgcg ggttgatcag
      301 cgtcgtcacc gccgtgccgt cgtcgaacgc gacggccagc tcgaccggtc gcggtcgcga
      361 catccggccc tcgtcgccca ccgtcaacat cccgatcgcg cagtatcggc gctcgccgga
      421 gtcgacgacg gatgttcgca ggaaccgcgc gatgcgacga tcggtcttca aatccttcgg
      481 cagcactgga cgtttcagga cgagaccggc caacgaggtg cagcggctca gcgccacata
      541 gagctgtccc gtcgagaaca ttccgccggt caggtcgacc accagacgct ccagcgtctg
      601 tccctggctt ttgtggatcg tgatcgccca cgcgagcttg aacggcagct gcgtgtacga
      661 gccgatcact tcccggccca atgagccgcc gccgaccacg ggtcgcgtcg cctcccagga
      721 gtacggcgtc acgtccgcgc aggacccgtc ggcgaactcg acttcgacga cggcaccgta
      781 gcggtcgtag tcgacgccca tcacccggcc gatggtgccg ttgacccatc ggtcgctctg
      841 gtcgttgttg agcatcatca cctgagcgcc gaccttgaac cgcagcgtct cctcgacggg
      901 tgggtcgaac agcgacaggt ctccgtagac ccgtgcgtgg tggaccaatt cgtcgcccgg
      961 cagtcgttcg agctgctggc gattgcgggc cgtcaccagg cggttggtcg gcgccaatgt
     1021 cagccagaac tcgtcgtcgg gcggaacgaa atcggcatcg gcgcgggcat tgagctgctc
     1081 ctgggcgtgc cctagcagga caccttcgcg aatctcgttc agaatggccg tcatccggtc
     1141 gtcgccgagc tgacggaaca ccgttgtcaa cgagacggtg gggaagtcgt cgcggcggaa
     1201 actgtgcgcc gagaagaagt acggcgtctc gtacgtcgtc gaaaagaagt gcgcttcgtc
     1261 ctcggtgacg accggcggca gttgatagag gtcgccgacc aacaccagct ggacgccacc
     1321 gaacggcgta cccgggtccg gaccgaaacg cgacagtgcc gacgccacca tgtcgaacac
     1381 gtcggcgcgc accatcgacg cctcgtcgat gatcagggtc tgcagcgacg cgagtgtctt
     1441 ggcgaagcgg ccgggccggt actcgccgct gcggacaccg tccagcgtcg tcgtcgtgcg
     1501 gaaaccgaac agccggtgga tggtgtaccc gtcgacgttc aaggccgcga tgcccgtcgg
     1561 cgcaacgacg accaccttgc ggtcggtctc ggccatgaaa tggcggatca gcgtcgactt
     1621 gcccgtgccc gccttgccgg tcaggaagac atgcctgccg ccggccagca attcgagcgc
     1681 ttggcgaaac tcgtcggtga ggacgagccc gtcggtcagc cccgccatga gcacagatta
     1741 ctggccgccg cc
//
LOCUS       DASYLX010000538         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1562913,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000538 DASYLX010000000
VERSION     DASYLX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562913"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VGP27_13000"
     CDS             complement(<1..817)
                     /locus_tag="VGP27_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7852252.1"
                     /translation="MSVPARSAPLFADIDDVARRLTETGYLPDTATATAVFLADRLGK
                     PLLVEGPAGVGKTELARAVAQATGSGLVRLQCYEGVDEARALYEWNHAKQILRIQAGH
                     GDWDQTKTDVFSEEFLLTRPLLTAIRRTEPTVLLIDETDKADIEIEGLLLEVLSDFAV
                     TVPELGTIKAERTPFVLLTSNATRELSEALKRRCLFLHIDFPDADLERRILLSRVPEL
                     PEHLAEELVRIIGVLRGMQLKKLPSVAETIDWGRTVLALGLDTIDDELIAATLG"
     gene            complement(865..2112)
                     /locus_tag="VGP27_13005"
     CDS             complement(865..2112)
                     /locus_tag="VGP27_13005"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV7852253.1"
                     /translation="MSLPAPSVPDLRQQVHEAARWARVAARELATLSTAAKNSALHAA
                     ADNVLRDASVVLEANKEDLARAKAAGTPDAMLDRLALDPQRIDGIAAGLRQVAGLPDP
                     IGEVLRGRTLPNGLQLRQQRVPIGVIGMVYEGRPNVTVDAFGLTFKAGSAVLLRGSSS
                     AARSNSALVTALRDGLQIENRNADAVQLLPSHDRASVTHLIQARGLVDVVIPRGGAGL
                     IEAVVRDAQVPTIETGVGNCHVYVHESADLDVAERILLNAKTRRPSVCNAAESVLIDA
                     AIADRAVPRLTQALQDAGVTVHGDPSEEELRAEFLSMDIALAVVDGLDAAIAHVNEYG
                     SGHTEAIVTTDLAAAQRFTERVDSAVVMVNASTAFTDGEQFGFGAEIGISTQKLHARG
                     PMGLPELTSTKWIVWGDGHTRPA"
     gene            2176..>2685
                     /locus_tag="VGP27_13010"
     CDS             2176..>2685
                     /locus_tag="VGP27_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV7852254.1"
                     /translation="MAAQVCVVGSVNMDHVFTVESLPHPGETVMASSVRLLPGGKGGN
                     QAVAAARAGAAVQLVAALGDDPAGDELRAHLRTNGVGLDGLFGVPGPSGSAVIAVDSA
                     GENTIVVAPGANARLTVESSQARAVIADSDVLLLQLEIPVTTAVEAARVARDAGTVVV
                     VNASPAGAKP"
BASE COUNT          428 a          904 c          901 g          452 t
ORIGIN      
        1 caccgagcgt ggcggcgatc agctcgtcgt cgatggtgtc cagacccagc gccagaaccg
       61 tgcggcccca gtcgatggtc tcggcgaccg acggcagctt cttgagttgc atgccgcgca
      121 gcacaccgat gatccgcacc aactcctcgg ccaggtgctc gggcagctcg ggcactcgcg
      181 acaacaggat tcggcgctcg aggtcggcat ctggaaagtc gatgtgcagg aacaggcagc
      241 ggcgcttgag ggcctcggac agctcgcggg tggcgttgga ggtcagcagc acgaacggcg
      301 tgcgctcggc cttgatcgtg ccgagttccg gcactgtgac cgcgaagtcg gacagcacct
      361 ccagcagcag gccctcgatc tcgatgtcgg ccttgtctgt ctcgtcgatc agcagaacgg
      421 tcggctcggt ccggcgaatt gccgtcaaca agggccgcgt cagcaggaac tcttccgaga
      481 acacatcggt tttcgtctgg tcccagtcgc cgtggccggc ctggatccgc aggatctgct
      541 tggcgtggtt ccactcgtag agcgcgcggg cctcgtcgac accctcgtaa cactgcagcc
      601 gcaccagacc cgatccagtc gcctgggcga ctgcgcgggc cagttccgtt ttgccgacgc
      661 ctgccggccc ctcgaccaac agcggcttac ccagccggtc ggcgaggaaa accgcagtgg
      721 ccgttgcagt gtcgggcagg tagccggtct cggtcagccg tcgcgcgaca tcgtcgatgt
      781 cggcgaacag cggcgccgag cgtgcgggga cactcacgtg ctgggtccga ttctgttcgc
      841 gcaagcgctc atcgttctcc taattcaggc cgggcgagtg tggccgtctc cccacacaat
      901 ccacttggtc gaggtcaatt ccggtaggcc catcggtccg cgggcgtgca gcttctgggt
      961 ggagatgccg atctcggcgc cgaagccgaa ctgctcaccg tcggtgaatg ccgtcgatgc
     1021 gttcaccatg acgacagcgg aatcgacgcg ctcggtgaac cgttgggcag cagcgagatc
     1081 cgttgtgacg atggcctcgg tatgcccact gccgtattcg ttgacgtggg cgatcgcggc
     1141 gtcgaggccg tccaccaccg ccagcgcgat atccatcgac aggaactcgg cgcgcagttc
     1201 ctcctcggag gggtcaccgt gtaccgtcac gccggcatcc tgaagcgctt gcgtcaggcg
     1261 cggcaccgcg cggtcggcga tggccgcgtc gatcaatacc gattctgccg cattgcagac
     1321 actcggccgc cgcgtcttgg cgttgagcag gatgcgttcg gcaacgtcga ggtcagccga
     1381 ttcatgaacg tagacatggc aattgccgac acccgtctcg atggtgggca cctgcgcatc
     1441 gcggaccacc gcctcgatga ggcccgctcc cccccgcgga atgacaacgt cgaccaagcc
     1501 gcgggcctgg atcagatggg tgacgctggc ccggtcgtgg ctgggcagca gttgcacggc
     1561 gtcggcattg cggttctcga tctgcagtcc atcccgcaac gcggtaacca gcgctgagtt
     1621 cgaccgcgcc gccgatgagc tgccacgcag aagcaccgcg ctgcccgcct tgaatgtcag
     1681 gccgaaggcg tcgacggtca cgttcggcct gccctcgtag accattccga tcacgccgat
     1741 cggcacgcgc tgctgacgca gctgcagacc gttgggcagc gtgcgcccgc gcaacacctc
     1801 accgatcggg tcgggcaggc ccgccacctg ccgcagcccg gcggcgatgc cgtcgatccg
     1861 ctgcgggtcg agcgcgagac gatcgagcat cgcatcgggt gtgccggcag ccttggccct
     1921 ggcgagatcc tctttgttcg cttcgagcac gacggaggcg tcgcgaagca cgttgtcggc
     1981 ggcggcgtgc agggccgagt tcttggctgc cgtgctcagg gtcgccagtt ctcgggcggc
     2041 caccctggcc caccgcgccg cctcgtgcac ctgctggcgt aagtcgggca ccgacggtgc
     2101 gggcagactc attaccccag cgtatcgaac gtcaatcaag cgctcgcagc ggtgcttggt
     2161 ctaacttcta cgtctatggc ggcgcaggtg tgcgtagtgg gcagcgtcaa catggaccac
     2221 gtcttcaccg tcgagtcgct accgcacccc ggtgagacgg tcatggcatc atcggtgcgc
     2281 ttgcttccgg gaggcaaagg cggcaatcag gcagtcgcgg cggcgcgcgc gggagcggcc
     2341 gtacagctcg tcgcggcact cggcgacgat cccgcaggtg acgagctacg tgcccacctg
     2401 cggaccaacg gtgtcggcct cgacggtctc ttcggagtgc cgggcccgag cgggtcggct
     2461 gtcatcgcgg tcgactccgc gggcgagaac accattgtcg tcgcaccggg cgccaacgcg
     2521 cgactgacag tcgagtccag ccaggccagg gccgtcatcg ccgacagcga cgtgctgctg
     2581 cttcagctgg agatccccgt cacgaccgcc gtcgaggcgg cacgagtggc gcgcgacgcg
     2641 ggcacggtgg tggtcgtcaa cgcatcgccc gcaggcgcga agccg
//
LOCUS       DASYLX010000539         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2767521,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000539 DASYLX010000000
VERSION     DASYLX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767521"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..139
                     /locus_tag="VGP27_13015"
     tRNA            67..139
                     /locus_tag="VGP27_13015"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:His,seq:gtg)
     gene            291..1589
                     /locus_tag="VGP27_13020"
     CDS             291..1589
                     /locus_tag="VGP27_13020"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7852255.1"
                     /translation="MHSIARIAVLTTCAAIVAGCTSTSSTTPAGSGTTTTAASPSVSS
                     ALKPIDQAALQKVVEKAVKEMLVPGAVVAIRTPQGSYTAAVGTTELGKQTPPDTNTHF
                     RIASNTKTMTAALVVLLAQDGKLKFSDPVSDYVPDVPDGTHITVADLLKMRSGLYNYT
                     NAPEFSAQLDADPAKAWTPQEVLASAFARPANAAPDTEYEYNNTNYALLGVIAEKAGG
                     RPLGEQLHDRLFGRLGLQQTSLPANDDTAIPTPYSHGYMYGGSAYAMVDDPYPADLKA
                     AGRAGTLKPVDYTHQNPSYAHAAGGVISTADNLAAWMRALVSGKVFNADYQQQWRDSL
                     RAEDPNNRDGQKYGYGISYQRFSPTAAMYYHGGEMPGFNSFMGYDPDADVSLVIWTNL
                     TISLDDKTTAQALLPTVLDEIYTGLSLAPTADTTTTSATR"
     gene            complement(1597..2820)
                     /locus_tag="VGP27_13025"
     CDS             complement(1597..2820)
                     /locus_tag="VGP27_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7852256.1"
                     /translation="MWQVQEVARPRRFRACLMAIALVSALVLGLSGCGGNSAPPQPQV
                     INEKGTPFGDLLVPKLTASVKDGAVGVTVDQPVTVGAEDGVLGAVTMVNDDEGTPVAG
                     ELSPDGLTWHSTEPLGYNTRYTLNAQSLGLGGAANQQMTFQTHSPENLTMPYVLPNDG
                     EAVGIGQPVAVRFDENIPNRLAAQRAITVTTNPPVEGAFYWLSNREVRWRPAQYWKPG
                     TTVDVAVNTYGVDLGDGLFGQENVTTHFTIGDEVIATADDNTKTLTIRRNGEVVKTMP
                     ISMGKNSAPTNNGTFIVGDRFGHMVMDSSTYGVPSNSPNGYRTEVDYATQISYSGIFV
                     HAAPWSVGSQGYSNVSHGCLNVSTSNAQWFYNNTKRGDIVEISNTVGSILPGVDGLGD
                     WNIPWDQWRAGNANI"
     gene            2979..3051
                     /locus_tag="VGP27_13030"
     tRNA            2979..3051
                     /locus_tag="VGP27_13030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3012..3014,aa:Lys,seq:ctt)
     gene            complement(3100..3325)
                     /locus_tag="VGP27_13035"
                     /pseudo
     CDS             complement(3100..3325)
                     /locus_tag="VGP27_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110336.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3562..>3681)
                     /locus_tag="VGP27_13040"
     CDS             complement(3562..>3681)
                     /locus_tag="VGP27_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein"
                     /protein_id="HEV7852257.1"
                     /translation="TVRARTDAVLVGYTGRAFRERLGPAGVRDLIEHRELVIN"
BASE COUNT          665 a         1193 c         1159 g          664 t
ORIGIN      
        1 gacggaatgg attcggccgc cgagcgcacg agcggctagt atcgacgggc cgcatgtgcg
       61 gcgatggtgg ctgtagttca gttggtagag caccaggttg tgatcctggc tgtcgcgggt
      121 tcgagtcccg tcagccaccc cggacaagtc ggagggcttt ctgccctccg actttttggt
      181 tttcggcgct ttcgccgctt gctcgccgcc gagctgcggt ttcgctacat cgagacggtc
      241 gccgacttag gctccgctct gggtcaggtg tttcagcgag gagtgccgcc atgcacagca
      301 ttgcgaggat cgccgtgctc acgacgtgtg ccgcaatcgt cgccgggtgc acgtcgacgt
      361 cgtcgacaac gccggccggg tcgggcacga cgaccacggc tgcttcgccg tccgtctcgt
      421 cggcgctcaa gcccatcgat caggcggccc ttcagaaggt cgtggagaag gccgtcaagg
      481 agatgctcgt ccccggcgcc gtcgtcgcga tccgcacacc gcagggcagt tacaccgcgg
      541 cggtcggcac gaccgaactg ggcaagcaga caccgccgga tacgaataca cacttccgga
      601 tcgcctcgaa cacgaagacc atgaccgccg cactggtcgt gctgttggct caggacggca
      661 agctcaagtt cagcgacccg gtgtccgact acgtgcccga cgtccccgac ggcacccaca
      721 tcactgtcgc cgacctgctg aaaatgcgca gcgggctcta caactacacc aacgcaccag
      781 agttctctgc ccaactcgac gccgaccccg cgaaagcctg gacaccgcag gaggtgttgg
      841 cgagcgcgtt cgcgcgtccg gccaacgccg cgcccgacac cgagtacgag tacaacaaca
      901 ccaactacgc gctcctcggg gtgatcgccg agaaggcagg cggccgtccg ttgggcgagc
      961 agttacacga ccgccttttc ggacggctcg gactgcagca gacatcgctg ccggcgaacg
     1021 atgacaccgc gatcccgacg ccgtactcac acggctacat gtacggcggt tccgcctacg
     1081 cgatggtcga cgatccctac cccgccgatc tgaaggccgc cggacgggcc ggaacactaa
     1141 agccggtcga ctacacgcac caaaacccct cctacgcgca cgccgctggc ggcgtaatct
     1201 ccacggccga caacttggcg gcatggatgc gagccctggt ctcgggcaag gtcttcaacg
     1261 ccgactacca gcagcagtgg cgagacagcc tgcgagccga agacccgaac aatcgcgacg
     1321 ggcagaagta cggctacggc atcagctatc agcggttcag cccgaccgcc gcgatgtact
     1381 accacggtgg tgagatgccg ggcttcaact cgttcatggg ctacgacccg gatgccgatg
     1441 tgtccctggt catttggacc aacctgacca tttcgctgga cgacaagacg acagcgcagg
     1501 cattgctgcc gacagtcctc gacgagattt acaccggtct gtcgctggcg cctacggcgg
     1561 acacgacgac gacgtccgct acgcgttagt cagacctcag atgttggcgt tgccggcccg
     1621 ccactggtcc cacgggatgt tccagtcgcc gagaccgtcc actccgggaa ggattgagcc
     1681 gacggtattg gagatttcga cgatgtcgcc acgcttggtg ttgttgtaga accactgcgc
     1741 attgctggtg ctcacgttca ggcagccgtg gctgacattg ctgtagccct ggctgcccac
     1801 cgaccacggc gcagcgtgaa cgaagatgcc gctgtaggag atctgcgttg cgtagtccac
     1861 ctcggtgcga tacccgttgg gcgagttcga cggcacgccg taggtcgagg agtccatgac
     1921 catgtgcccg aaccggtcgc cgacgatgaa ggtgccgttg ttggttggcg cgctgttctt
     1981 gcccattgag atcggcatgg tcttgacgac ttcgccgttg cgccggatgg tcagcgtctt
     2041 ggtgttgtcg tcggcggtcg cgatcacttc gtcaccgatg gtgaagtgcg tagtcacgtt
     2101 ctcctggccg aacaggccgt cgccgaggtc gacgccatag gtgttgacgg cgacgtccac
     2161 ggtcgtgccc ggcttccagt attgggctgg gcgccaacga acttcacggt tgctgagcca
     2221 gtagaaggcg ccctcgacag gtgggttggt ggtcacggtt atggcccgct gcgcggcgag
     2281 ccggttcggg atgttctcgt cgaagcggac cgcgacgggc tgcccgatgc cgacggcctc
     2341 gccgtcgttc ggcaacacat acggcatggt caggttctct ggcgagtgcg tctgaaaagt
     2401 catctgctgg ttggcggcac cgcccaggcc cagcgactgc gcgttgagcg tgtagcgcgt
     2461 gttgtagccc agcggctcgg tgctgtgcca cgtcagaccg tcggggctga gctcaccggc
     2521 gaccggcgtg ccctcgtcgt cgttgaccat cgtgaccgcc ccgaggacac cgtcctcggc
     2581 gccgaccgtc acgggctgat ccaccgtcac gccaacggcg ccgtccttca ccgacgcggt
     2641 gagtttgggc accagcaggt cgccgaacgg cgtccccttc tcattgatga cctgtggttg
     2701 cggaggagca gagttgccac cgcagccgct gaggccgaga actagggccg ataccagcgc
     2761 gatggccatc aaacaggcac ggaaacgacg cggacgggcc acctcttgga cctgccacat
     2821 aattgctcca actcataacg ggacgtgctc gtcgggattc ttggcaatag tctaagggaa
     2881 agctgagtgt ctatgcgact tcactgagag tctccacagg cgggagccca gaaagggatt
     2941 tcacccagca ccgcagggcc tgttattgtc gcacacgcgc gccgttagct cagttggtag
     3001 agcagctgac tcttaatcag cgggtccggg gttcgaaacc ctgacggcgc acagctcaaa
     3061 ggtggtacct agccatcctt cggaccgcga ggtcaccgtt tattcaccgc ttatgactgc
     3121 ccggtcgagg agggcagcaa cctcggtgcg gactcgaccg cgagccatat aacggtcctg
     3181 tgtcatcgac accttggcgt gcccccagat ggtccgcgcc aacccgcgcc gacaaccctt
     3241 cgtcatggat gagggtggca acggacttgc ggaagctgtg cgacgtcacg tccgggaggt
     3301 cgaactggtc cctcacaagc cgccaaccga tcgatgtggt tgccgacgag cacggtcacc
     3361 ctggtgtcaa ggttgatctc ccctatcgtt ggcgcctgac gcgacgcaag cgttttccgc
     3421 cgccatgacg gattatcatg caccgacagc gccagcggcg acgcgaattt ggctgcctag
     3481 gcgagcgccg cattaacacc gcatgactgg tggaccggca gcctcgcacg attacgcacc
     3541 gcggctggcg ctccggccgg attagttgat gacgagttcg cggtgctcga tcaggtcacg
     3601 cactccggcc ggtccgagac gctcgcggaa agctcgcccg gtatagccga ccagcaccgc
     3661 atcggtccgt gcccgcacgg t
//
LOCUS       DASYLX010000540         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2987711,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000540 DASYLX010000000
VERSION     DASYLX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987711"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..379)
                     /locus_tag="VGP27_13045"
     CDS             complement(74..379)
                     /locus_tag="VGP27_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEV7852258.1"
                     /translation="MEFLVDMVTTVPEGTSGETVEDTKRREAARTEELAAQGHLLRLW
                     KPPEAPGEWRTFGLFNATDEDELRQVLASMPLHEWMTVTVTPFTPHPSDPGIGRPAG"
     gene            complement(394..789)
                     /locus_tag="VGP27_13050"
     CDS             complement(394..789)
                     /locus_tag="VGP27_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7852259.1"
                     /translation="MTSTETDVLKTVLDQWKSAVDAHDPKRVATYFTDDAIFQGLHPY
                     SVGPDSVAAYYDSQPIGMTTDYTVLQTRRLADDLVLGYLSVDFGFTDRPTLTVYLSVI
                     ARRTGESWLINHYQVSRLGDSDGNHSVHS"
     gene            complement(834..1541)
                     /locus_tag="VGP27_13055"
     CDS             complement(834..1541)
                     /locus_tag="VGP27_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852260.1"
                     /translation="MSTQKVAVITGASQGIGAALVVAYHKLGYAVVANSRSITPSGDP
                     DILAVAGDIADPAVGARVIEQALATFGRVDALINNAGVFVAKPFTDYTNADYDVVTGV
                     NLRGFFEVSKRAIAAMLDGEGGHVVNISTSLVDHANSAVPSALAALTKGGLNAVTKSL
                     AIEYAARSIRVNAVSLGVIRTPMHPAETHDTLAGLHPLGRMGDISDIVDAIVYLENAP
                     FVTGEILHVDGGQSAGH"
     gene            complement(1621..2265)
                     /locus_tag="VGP27_13060"
     CDS             complement(1621..2265)
                     /locus_tag="VGP27_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7852261.1"
                     /translation="MASATVRLIGGPTLVIEAGGLRLLTDPTFDQPGPFDVVLLSHDQ
                     HGDDPGLLERVPLVLTTSDAAQRLGGSARALPPWYHLSLPRFDGVALRITGVPAQEGP
                     NGTTHTGFVLSSVDTPAVYISGGNVSLDVVQQVAQRCAPIGIAVLFVDGYLTLTSDQA
                     ARAAAILASPTVIPAHVEGWDHYTECIDDVRAAFARHGLSDRLRVLAAGDTATI"
     gene            2358..>2890
                     /locus_tag="VGP27_13065"
     CDS             2358..>2890
                     /locus_tag="VGP27_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7852262.1"
                     /translation="MELRQLRYFVAVAEELNFGRAASRLLIASPSLSQQIKALERDLG
                     VRLFDRDRRSVALTECGAALLPKTRALLAQADELRRSASGMAASEPVRLGYVNWRPAD
                     LLARVSGVAQLHVDTWVLPSHAQARRVADHSIDLAICWVRTGDLARHGLQARLIGADR
                     LYTISVGADTAEVHARDT"
BASE COUNT          419 a          909 c         1042 g          520 t
ORIGIN      
        1 tgatgggatg acatgggcgg cgcgcttgag cacccggctc gacggtcgtt gcccttgctt
       61 ggtgtcgtcg ccgtcagccg gccggacgtc cgatgcccgg gtcgcttggg tgtggcgtga
      121 acggcgtgac cgtgacggtc atccactcgt gcagcggcat cgaggcgagc acctggcgca
      181 actcgtcctc gtcggtggcg ttgaacaggc cgaaggtgcg ccattcgccg ggcgcctccg
      241 gtggcttcca cagccgcagc aggtggcctt gtgccgcgag ttcctccgtc cgcgcggcct
      301 cgcgtcgttt ggtgtcctcg acggtctcgc cggaggtccc ttcggggacg gtggtcacca
      361 tgtcaacaag aaattccatg gccgtgctcc tgttcagctg tgaacgctgt ggttgccgtc
      421 tgagtcgccg agccgggaca cctggtagtg gttgatgagc caggactcgc cggtgcggcg
      481 cgcgatgacg ctgagatata cggtcagcgt cggccggtcg gtgaagccga agtcgacgct
      541 gaggtagccg aggaccagat cgtcggccag tcgccgggtc tgcaggacgg tgtagtccgt
      601 ggtcatgccg atgggctggc tgtcgtagta cgcggccacg ctgtccgggc cgacgctgta
      661 cgggtgcagg ccctggaaga tcgcgtcgtc ggtgaagtac gtcgcaacgc gcttcggatc
      721 gtgtgcgtcg accgccgatt tccactggtc gagcacggtc ttgaggacgt cggtctcagt
      781 gcttgtcatt atggttccta tgtctgtggc agctgaaaat cggctatgga tgctcaatgg
      841 cccgcgctct ggccgccgtc gacgtgcagg atctcgccgg tgacgaacgg agcgttctcc
      901 aggtagacga tcgcgtcgac gatgtcgctg atgtcgccca tccggccgag cgggtgcaga
      961 cccgcgagcg tgtcgtgcgt ttcggccggg tgcatgggtg tgcggatgac gcccagagac
     1021 acggcgttga ccctgatgct gcgcgccgcg tactcgatcg ccagcgactt cgtgaccgcg
     1081 ttcaggccgc ccttggtcag tgccgccagg gcggacggca cggcggagtt ggcgtggtcg
     1141 accaagctgg tcgagatatt gacgacgtgc ccgccttccc cgtcgagcat cgcggcaatg
     1201 gcccgtttcg agacctcgaa gaagccacgc aggttcacac cggtcaccac gtcgtaatcg
     1261 gcgttggtgt agtcggtgaa cggcttcgcc acgaagacac cggcattgtt gatcagcgcg
     1321 tcgacgcggc cgaaagtggc cagcgcctgc tcgatcacgc gggcgccgac cgccgggtcg
     1381 gcgatgtcgc ccgcgacggc caggatgtcg ggatccccgg acggggtgat ggagcgcgaa
     1441 ttcgcgacca cggcgtagcc gagcttgtgg taggcgacga ccagagccgc gccgatgccc
     1501 tgcgaagcgc cggtgatgac ggcaaccttc tgggtgctca tgacagtagg tctccttgtc
     1561 ttataggcgt gcggtatcca caccgcggtg cgcggtgctg tcttggggtg tgagttccgg
     1621 ttagatggtg gccgtgtcgc cggccgcgag gacccgcaac cggtcactga gtccgtgccg
     1681 ggcgaacgcg gcccggacgt cgtcgatgca ttccgtgtag tggtcccagc cttcgacgtg
     1741 ggccgggatg acggtgggcg aggccaagat cgccgcagct cgggccgcct ggtcactggt
     1801 gagggtcagg tagccgtcca cgaacagcac ggcgatgccg atcggcgcac agcgttgcgc
     1861 cacctgctgg acgacgtcca gggagacgtt gccgccgctg atatagacgg ctggcgtgtc
     1921 cacactggac agaacgaatc cggtgtgtgt ggtcccgttc ggtccttcct gcgccggtac
     1981 tcccgtgatg cgcagtgcga cgccgtcgaa acggggcagg ctgaggtggt accacggcgg
     2041 cagggcgcgc gcggatccgc cgagccgctg agcggcgtcc gaggtggtca gcacgagcgg
     2101 cacccgctcc agcaagccgg gatcgtcgcc gtgctggtcg tgtgacagca gcaccacgtc
     2161 gaacgggccg ggctggtcga atgtcgggtc ggtcagcagc cgcagtcccc cggcctcgat
     2221 caccaaggtg gggccaccga tcagccgtac cgttgcgctt gccatatccc gatagtgagc
     2281 cgggtgcggg ctcgttcgcc acgaccggct tgctctctgg tggtaggcgc agcctcctac
     2341 cgcactaggc tcgggacatg gagctacgcc agcttcgcta ctttgttgcc gtcgccgagg
     2401 agctgaactt cggtcgggcg gcgagtcggc tgctcatcgc cagtccctcc ctgtcccagc
     2461 agatcaaggc gctggaacgg gatctgggtg tgcgcctgtt cgaccgcgac cgccggtcgg
     2521 tcgccctcac cgagtgcggt gcggcgttgc tgccgaagac ccgtgcgctg ctggcgcagg
     2581 cggacgagtt gcgccggtcg gcgtcgggga tggccgcgag cgagccggtg cggctcggtt
     2641 acgtcaactg gcgtcccgcc gacctgctcg cgcgcgtgtc gggtgtggcg cagctacatg
     2701 tggacacgtg ggtcctgccc tcgcacgctc aggcgcgccg ggtcgccgac cacagcatcg
     2761 atctggcgat ctgctgggta cggaccggcg acctggcgcg gcacggcctg caagcgcggc
     2821 tgatcggcgc cgaccggctc tacacgatca gtgtcggagc cgacaccgcc gaggtgcacg
     2881 cgagggacac
//
LOCUS       DASYLX010000541         5697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2219366,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000541 DASYLX010000000
VERSION     DASYLX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219366"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..834
                     /locus_tag="VGP27_13070"
     CDS             1..834
                     /locus_tag="VGP27_13070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852263.1"
                     /translation="MSASLRRRIGLAFLLIPPLAWLVLAYLGSLAVLLVSAFWSTNSF
                     TGAVVHKFTTHNIVQAFSDTVFRVTTLRTIGLALLVTAICIVLAVPLALYMAKMASPR
                     MRLLLVIAVTTPLWASYLVKAYAWRMLLSPEGPLAWAGGYTPGYGLTATVITLAYLWL
                     PYMIIPVFAAFDRVPDSLIDASSDLGASDLSTLRMVVAPLVFPGIAAGSIFTFSLSLG
                     DYIAVTIVGGKTQLLGNLIYGQLVTANNQPLAAALSIIPLAAIVLYLLAMRRTGALEN
                     V"
     gene            834..1634
                     /locus_tag="VGP27_13075"
     CDS             834..1634
                     /locus_tag="VGP27_13075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852264.1"
                     /translation="MLSSGARRAAGAWTVLIALFLYVPLALVLVNAFNSSRTFAFPPT
                     GFTTRWWVTAWHSEGMWKSLANSVVVGLGATAIALVLGTMAAFAVQRYRFFGREVVSF
                     LVVLPITLPGIVTGIALNATFTSALGMTLGLATVIVGHATFCIVIVFNNTQARLRRLG
                     AGLEEASADLGASPWQTFRYVTFPMMRGALVAGAILAFALSFDEVVVTTFTAGPTVQT
                     LPIWIFGNLFRPNQAPVINVVAAALTLVAIIPVWLAQRFGGDPATTRL"
     gene            complement(1636..2364)
                     /locus_tag="VGP27_13080"
     CDS             complement(1636..2364)
                     /locus_tag="VGP27_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7852265.1"
                     /translation="MAEYRLDDLARMSGVSSRNIRAYRERGLLDPPRRQGRSAFYDDY
                     HLSQLRTINQLLRKGFNSSHIAEFFARMREGHDLAAILGVQKAILGSTAHETRTAGAL
                     DIDPGCDEARRLMECGLAELSENSIALVDPQMADIVTRSTDQLSYVQMILRVVDSTSG
                     TFDQLAAALVAALDESVATRYGAGYVPTPGEMDDVTTLIQDYRELGIAAVAHQLDNGL
                     QGHMVSAVSDYAAGTVVWWKPTAY"
     gene            2690..3967
                     /locus_tag="VGP27_13085"
     CDS             2690..3967
                     /locus_tag="VGP27_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852266.1"
                     /translation="MRPWIVWATGLLAYIVAVLDRTTLGVSGLEAADRFTASPGVLSS
                     FVVLQIVVYAGAQVPAGLLLDRFGSKALIVSGATLMAAGQLTLAFTESLPAAIAARAV
                     VGLGDAVTFISVLRLVPHWFEPRQVPLVTQLTGICGQLGQVLSAVPFLALLAGEGWTT
                     AYVSVVALGVVSVALTLALVKNTPNGAVADPPSSSVREVWASVKTVWLRPGTRLGFFT
                     HMGTQFSVTAFALMWGVPYLTVGQGLSIHAAGTLLSLSVVAAIVAGILIGIFTGRRPH
                     RRSRLVLAIMASNAAIWTVVLALPTAAPLWLLALLVIVISVGGPGSMVGFDFARTFNP
                     SATLGTAQGMVNMGGFLASLLVMQTMGLVIGAVGGYSFESFRLAWTVQYAIWALATIG
                     ILVTRKKARRLMRQQPDLLEGFDSHATEPAPAR"
     gene            complement(3973..4392)
                     /locus_tag="VGP27_13090"
     CDS             complement(3973..4392)
                     /locus_tag="VGP27_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEV7852267.1"
                     /translation="MPKTTSRGTAKKNELPSTLQKSSAKAQRTFAKAHDAAAEEYGEG
                     ERAHRVAYSALKHSFEKVGDHWEPKKQKGPSDARARSGGPNAKGSSAEGVDANASKTH
                     LMDVARRLDIPGRSSMTKGQLVEAIKKANRRVSSRNR"
     gene            complement(4555..>5697)
                     /locus_tag="VGP27_13095"
     CDS             complement(4555..>5697)
                     /locus_tag="VGP27_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEV7852268.1"
                     /translation="SASEFAHALAEDHVGAGLAGFAFATFAICWAWINFSWFASAYDT
                     DDWIYRLTTMLQMVGVIILALGIPAMYATVAQGEHVDNRVMVAGYVVMRIALVAQWLR
                     AAKQDPQRRAACLTYATVVTVAQIGWIVMIVVSTSVVATFAWSAVLVTFEMLGPWLAE
                     RRMGGTPWHAHHIAERYSLLTIIALGEGVVGTVASLSAVVSAQGWSVAAVLVAVAGTG
                     LTFGMWWVYFIVPQSQLLHARRELSFRFGYLHLVVFGAIVGTGAGLHAAAYYVEHHSK
                     LGAVGTVLSVAIPVAVYIAAVYLLYLWLLRARDAFHLLLVVLTVVVLIAAVVLVGRGM
                     SMANALLIVMLAPAVTVVGYELLGYRHAEAAIAERLANGPSGKPSA"
BASE COUNT          909 a         1898 c         1841 g         1049 t
ORIGIN      
        1 atctcggcgt cactacgacg ccggatcggg ttggccttcc ttttgatccc gccgctggcc
       61 tggctggtgc tggcgtatct gggatcgctt gcggtgcttc tggtttcggc attctggagc
      121 accaacagct tcaccggcgc ggtggttcac aaattcacca cccacaacat cgtgcaggcg
      181 ttcagcgaca cggtgttccg ggtcaccacg ctgcgcacga tcggactcgc cctgttggtc
      241 accgcgatct gcatcgtgct cgcagtgcca ttggcgttgt acatggccaa aatggcctct
      301 ccccggatgc ggctactgct ggtgattgcc gtgacgacac cgctgtgggc gagctatctg
      361 gtcaaggcgt acgcgtggcg gatgctgctc tcgccggagg gcccgctggc ctgggcgggc
      421 ggctacacac cgggctacgg actgacagcg accgtgatca cgctggccta cctgtggttg
      481 ccgtacatga tcattcccgt gttcgcggcg ttcgaccggg tgcccgactc gctgatcgac
      541 gccagctccg acctcggcgc ctccgacctc tccacgctgc ggatggtggt ggcaccgttg
      601 gtctttcccg gcatcgcggc aggttcaatc ttcacttttt cgctgtcact cggcgactac
      661 atcgcggtga ccatcgtggg cggtaagacc cagctcctcg gcaacctcat ctacggccag
      721 ctggtgaccg cgaacaatca gccactggcc gccgcgctgt cgatcattcc gctcgcggcg
      781 atcgtcctgt acctgttggc gatgcgccgc accggcgcgc ttgagaacgt ctgatgctct
      841 cgagcggcgc gcggcgtgcg gccggagcct ggaccgtgct catcgcgctg ttcctgtatg
      901 tgccgttggc gctcgtgctg gtcaacgcat tcaactcgtc gcggaccttc gccttcccgc
      961 cgaccggctt caccacgcga tggtgggtca ccgcatggca cagcgaggga atgtggaagt
     1021 cgctggccaa ctccgtggtg gtcggcctgg gggcgacggc gatcgcgttg gtgctcggca
     1081 ccatggccgc attcgcggtg cagcggtacc ggttcttcgg ccgcgaggtc gtcagtttcc
     1141 ttgtggtgct gccgatcacg ctgccgggca tcgtcaccgg tatcgcactc aacgcgacgt
     1201 tcacctcggc gctcggtatg acgctgggct tggcgacggt gatcgtcggg cacgccacgt
     1261 tctgcatcgt catcgtcttc aacaacacgc aggcacgcct gcgcaggctc ggcgccggcc
     1321 tggaggaggc ctccgccgac cttggcgcgt cgccctggca gacgttccgc tacgtgacct
     1381 tcccgatgat gcgcggcgcc ctcgtcgccg gcgcgattct ggcgttcgca ctcagcttcg
     1441 acgaggtcgt ggtgacgacg ttcaccgcgg ggccgacggt gcagacactg ccgatctgga
     1501 tcttcggaaa cctgttccgg cccaaccagg cgccggtcat caacgtcgtc gccgcggcgc
     1561 tgacgctggt ggcgatcatc ccggtatggc tggcccagcg attcggcggt gaccccgcaa
     1621 cgacacgcct gtgagctaat aggccgtggg cttccaccaa acgactgtgc cggcggcgta
     1681 gtccgagacg gcggacacca tatgtccttg cagcccgttg tcgagctggt gtgcgacagc
     1741 ggcgatgccg agctcacggt agtcctggat gagcgttgtc acgtcgtcca tctcgcccgg
     1801 cgtgggcacg tatccggccc cgtagcgagt ggcgacggac tcgtccagcg cggcgaccag
     1861 cgcggccgcc agctgatcga atgtgccgct ggtcgaatcg acgacccgca ggatcatttg
     1921 cacatacgag agctgatcgg tcgaacgggt gacgatgtcg gccatctgcg gatccacgag
     1981 ggcaatcgaa ttctcgctga gctcggcaag cccgcactcc atcagccttc gggcctcgtc
     2041 acagccgggg tcgatgtcga gcgcgccggc ggtgcgcgtc tcgtgggccg tgcttccgag
     2101 gatcgccttc tggacgccca agatggccgc gaggtcgtgg ccctcccgca tgcgagcgaa
     2161 gaactcggcg atgtgggagg agttgaagcc cttgcgcaga agctggttga tggttctcag
     2221 ctgggacagg tggtagtcgt cgtagaacgc cgagcgaccc tgccgacgtg gagggtcgag
     2281 caggccgcgc tcccggtacg cgcggatgtt gcgtgagctg acgcctgaca tccgggcgag
     2341 gtcgtcaagt cggtattcag ccaacccaac gcaccaaacg tcgacggggg cggccacctc
     2401 gcatgccgac actgtaatac cgaaaacgcg tttgcgagcg aagtcgcgat cgtttcaatc
     2461 agaaattcgc aattcggata gccacgccat tcatcagccc gtttggcgta ttagtgattc
     2521 acatcaccac acggatatgt gattacagcc aacttcacgg ggtggatgtc aggtattccc
     2581 ccgcggcggg tgagtgatct caccggtaat gaattggcgt ggcggcggcc cactgcggga
     2641 ccatggccat gccggtcatt aagacaaagt cagctatgag aggtttcgcg tgcgtccctg
     2701 gatcgtttgg gccaccggac tcttggcgta catcgtcgcc gtgctcgacc gcaccaccct
     2761 cggagtctcg ggcctggagg ccgccgaccg gttcaccgca agccccggcg tgctctcgtc
     2821 gttcgtcgtc ctgcagatcg tcgtctacgc gggcgcacag gtcccggcgg gcctgctact
     2881 ggacaggttc ggctccaagg ccctgatcgt gtccggcgcg acgctgatgg cagcaggcca
     2941 gctgacgctg gcgttcaccg agtcgctgcc ggcggcgatc gcagcccgcg cggtggtcgg
     3001 gctcggcgac gccgtcacat tcatctcggt gttgaggctg gtgccccatt ggttcgagcc
     3061 gaggcaagtc ccgctggtga cgcagctgac cggcatctgc ggccaactcg gccaggtgct
     3121 gtcggccgtt ccgttcctgg cgctgctggc gggagagggc tggacgacgg cctacgtatc
     3181 ggtggtcgcg ctcggcgtgg tgtcggtcgc gctgacgctg gctctggtca agaacacccc
     3241 gaacggtgca gtggcagacc cgccgtcgag ttccgttcgc gaggtgtggg cgagcgtgaa
     3301 gaccgtctgg ctgaggcccg gcacccgact gggcttcttc acacacatgg gtacccagtt
     3361 ctcggtgacc gccttcgcgt tgatgtgggg cgtgccctac ctgaccgtcg ggcagggttt
     3421 gtcgatccac gccgcgggga cgctgctgag cctgtcggtc gtcgcggcga tcgtcgcggg
     3481 cattctgatc ggcatcttca ccgggcgccg tccgcatcgg cggtcacgtc tggtgctggc
     3541 catcatggcg agcaacgccg cgatatggac ggtcgtgttg gcactgccga ccgcagcacc
     3601 actgtggctg ctggcgctgc tcgtcatcgt catctcggtc ggcggtccgg gttcgatggt
     3661 cggcttcgac ttcgcccgca cgttcaatcc cagcgccacc ctcggcaccg cccagggcat
     3721 ggtcaacatg ggcggcttcc tggcctcgct gctggtgatg cagacgatgg gcctggtgat
     3781 cggcgcggtc ggcggctact cgttcgagtc gttccgcctc gcgtggaccg ttcaatacgc
     3841 gatctgggcg ctggcgacca tcggcatcct cgtcacccgc aagaaggccc gcagactgat
     3901 gcgacagcag ccggatctgt tggagggttt cgacagtcac gcaaccgaac cggcgcctgc
     3961 gcgctgatcc cgctaccggt tgcggctgct gacccgccgg ttggccttct tgatcgcctc
     4021 caccaattgg ccctttgtca tgctggaacg acccgggatg tccagccgcc gtgcgacatc
     4081 catcagatgg gtcttcgacg cgttggcgtc cacgccttcc gccgaggatc ccttcgcgtt
     4141 cgggccgccg ctgcgggcgc gcgcgtcgga cggacccttc tgcttcttcg gctcccagtg
     4201 gtcgcccacc ttctcgaagc tgtgcttcag agcgctgtag gcgacccggt gcgcacgctc
     4261 accctcgccg tactcctcgg cggcggcatc gtgcgccttc gcgaatgtcc gctgcgcctt
     4321 cgcggacgac ttctgcagcg tgctcggcaa ctcgttcttc ttggcggtgc cgcgtgatgt
     4381 cgtcttcggc atagcctgct ccttcgtcga actacgagtg agagagccgc cgaagtcgca
     4441 tcgttgatga ttgccggctc cctgaagaac ggcggccgct tcttgaccag acccctcggc
     4501 tgctcgccat cgcgagactg tggtgccgct cacaccacga tgcgacctcg acgactacgc
     4561 agagggctta cccgaggggc cattcgctaa acgctctgcg atggccgcct cggcgtgccg
     4621 gtagcccagc agttcgtagc cgacgacggt cacggcgggc gcgagcatga cgatcaacag
     4681 tgcgttggcc atcgacatgc ccctgccgac cagcacgacg gccgcgatca gcaccacgac
     4741 ggtgagcacg acgagcagca gatggaacgc gtcgcgcgcc cgcagcaacc acaggtagag
     4801 cagatagacc gcggcgatgt agaccgccac gggtatcgcc accgacagca cagtgcccac
     4861 cgcgccgagc ttcgaatggt gctccacgta gtaggcggcg gcgtgcaacc cggcgccggt
     4921 gccgacaatc gccccgaaca ccaccagatg cagatagccg aaccggaagg acagttcgcg
     4981 tcgggcatgc agcagttgtg actgcggcac gatgaagtac acccaccaca tgccgaacgt
     5041 cagaccggta cccgcaaccg cgaccaggac ggccgccaca ctccagccct gcgccgagac
     5101 aaccgccgac aacgatgcaa cggtgcctac cacgccctcg ccgagcgcga tgatggtgag
     5161 cagcgaataa cgttcggcga tgtgatgggc gtgccacggg gtgccgccca tccgccgctc
     5221 ggcaagccac ggccccaaca tctcgaaagt caccaacacg gccgaccacg cgaacgtcgc
     5281 gacgaccgac gtgctgacca cgatcatgac gatccagccg atctgcgcga cggtgacaac
     5341 ggtggcatag gtaaggcacg ccgcacgtcg ctgcggatcc tgtttggccg cgcgtaacca
     5401 ctgagccacc aacgcgattc gcatcacgac atagcccgcc accatcaccc ggttgtcgac
     5461 gtgctcgccc tgggcgacgg tggcgtacat ggcgggaata ccgagcgcga ggatgatgac
     5521 gccgaccatc tgcagcatcg tggtcaggcg gtagatccag tcgtcggtgt cgtaggccga
     5581 cgcgaaccag ctgaaattga tccacgccca gcagatggcg aaggtcgcga atgcgaaccc
     5641 ggccagcccc gcgcccacgt ggtcctcggc gagcgcatgc gcgaactccg aggccga
//
LOCUS       DASYLX010000542         7841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3536125,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000542 DASYLX010000000
VERSION     DASYLX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536125"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..275
                     /locus_tag="VGP27_13100"
     CDS             135..275
                     /locus_tag="VGP27_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852269.1"
                     /translation="MQVVSRARSLDLLGAPVTEDAHSARHSTRNAEDFHGIEGLVDIV
                     AA"
     gene            complement(294..1328)
                     /locus_tag="VGP27_13105"
     CDS             complement(294..1328)
                     /locus_tag="VGP27_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEV7852270.1"
                     /translation="MARVETRVARIAEELLTERRYVRLVDVLVGLGWLAQPNVDRWER
                     GRVPSLDRCVAVDFDKVMTAIAALQDWAEHRGLTPSEADYQDLQFTVDGDLEAESAYR
                     TQWASSETPEIVVERPPCRPQQMITVIAPHNPWTCASCGDTGDFLRMDKAGALCLDCA
                     DLGHLEFLPSGDAALTRRATKASRLSAVVVHWSTRRKRYERQGILAEAAAIEQAAQEC
                     LSDADVRARRRTREECRRADQDVQFRSEFAAAIREQFPGCPLDRAEAIALHAAARGSG
                     RVGRSAAGRALDREAVRLAVVASVRHIDTDYDTLLMTGVDRESARAQVHQRVEDVVNA
                     WRGGAAMLDG"
     gene            complement(1380..3368)
                     /locus_tag="VGP27_13110"
     CDS             complement(1380..3368)
                     /locus_tag="VGP27_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEV7852271.1"
                     /translation="MSLRFDPDVPTYVDAAVWTACVADPTLFTDPLPPLSEIVEDVGI
                     AHLGEWLAPRGFDIVRWQLERRGELLAERHGLNAGDGFVLAALIELYDRAQRLIEADD
                     PGEADESAVLFADHGSIGVPEELGAQLVDPLLAELLRAETVGANRGSAAALGLLAELM
                     GSRAPRTARVAWRWLRAVALERVGDIDQAERELLAAESMDPDWPLPLLDLARVANDRG
                     DVERGLGLLRRADAGPGHPLVELLQAHRAEPRPDLGRNEPCWCGSGRKYKKCHLGREQ
                     LPLAQRVNWLYAKAVQYAQFSGWTDLLAEVSYERYRHTHDLAEALDAGMADMLVMDVV
                     LFEGGAFAEFLQVRGSLLPDDERLLAEQWHLVERSVFEVETVVPGEGLTVRDIRTGDV
                     HEVRERTASRQLKAGQLICARVVPAGDSMQFFGGAEPVTLHERDALIDLLDAEPDPVD
                     LISMVSRRFAPATLTNTEGDPLVLCEATVQVADPDKIAASLDETYDRVDGAEPPRWHE
                     QVNTRGMPKIRVVLVREGDMLRVETKSTERMDRVLATLIGLDPTMRVLDDVRRPVRDT
                     RGAAELASQLPAAEDALDTDAPEVAAMLDEFIREYETRWLDEPIPALDGHTPRQAADD
                     PTRRGDLIKLLDSFPPDEDKAGRMSVERLRTLLGLHGE"
     gene            3438..3764
                     /locus_tag="VGP27_13115"
                     /pseudo
     CDS             3438..3764
                     /locus_tag="VGP27_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
     gene            complement(3932..4978)
                     /locus_tag="VGP27_13120"
     CDS             complement(3932..4978)
                     /locus_tag="VGP27_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEV7852272.1"
                     /translation="MRRSASAKGPKTPKIVVFALYNKVTLQDVAAPLEIFARANDFGA
                     KYQVILASPTGEAVNTTSFVALKVDMPLAEVPDPIDTLIVPGGVPPDFNFTPGEHDIP
                     EEQTPDVVSAALEMVGELAPRARRVASVCTGAFVLAALGLLDGRRATTHWAHCRELAR
                     TYPDVEVDPDSLFVQDGRFITGAGISAGIDLGLAMVESDYGPDVARRVARWMVVFLQR
                     PGGQAQFSVWTESALPVSQGLREILDSVVADPAADHSIATMAGRAAVSERHLVRMFRV
                     QVGMTPARFVEQARLEAAKVLLATGDHGQEAVARRAGFGSADTMRRTFRRALGVSPSM
                     YRSRFRTTGIHQSV"
     gene            5268..5810
                     /locus_tag="VGP27_13125"
     CDS             5268..5810
                     /locus_tag="VGP27_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEV7852273.1"
                     /translation="MDSRTALTLSIGVLGGLAVALTAEVITVPIWVVFLSWASFFFVG
                     GGVAGWVRSVTSNIVGVVIASASLYAAYLMGGSLLVTAIAVGVGSALMVQASWVPLLA
                     TTPAVVVGFASTVSTVAGTGSDITVTTISHPGLVAAVACVLGASFGLLSEYVATAMTK
                     KSADQPAAGAESPSTEGSPA"
     gene            5810..6547
                     /gene="nthB"
                     /locus_tag="VGP27_13130"
     CDS             5810..6547
                     /gene="nthB"
                     /locus_tag="VGP27_13130"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEV7852274.1"
                     /translation="MKLQHYLGGLEGLPEPLTLEERVFVEEWEKRIFGIHVAMMGLSN
                     HLGSALPAYPIDEVPTAFNDEWTWADLRTGAEAMNPFDYFKFRYYEKWLGGITQFFVD
                     KGYVTEEELADRMADLPPAADAAGADTIDDQVIAYLRSGDSPRRDVAHPKFAVGSGVR
                     ITNVPADAHSRLPGYLRGRIGTVERVFEGDYAYFCHTGDGIGDPMPIYIVEFEPAEIW
                     GVRAEDGPLSLYAELFEAYLQPMEEKS"
     gene            6544..7164
                     /gene="nthA"
                     /locus_tag="VGP27_13135"
     CDS             6544..7164
                     /gene="nthA"
                     /locus_tag="VGP27_13135"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEV7852275.1"
                     /translation="MTGQFAYPPDREEASAKKAAALESLLIEKGVITPQTVDKVLAYF
                     ETEMTPLNGKKIVVKAWTDPEFAEKVVVDTPAAIAELDLPDGMAGAEGEHLQAVANNP
                     GVHNLVICTLCSCFPWPVLGLPPYWYKDPTFRSRAAREPRKVLAEVGLDLPADTEIKV
                     WDSSGHSRWFVIPERPTGTDDFTDEMLMNLVTTEAMIGVAQAGPVS"
     gene            7161..7472
                     /locus_tag="VGP27_13140"
     CDS             7161..7472
                     /locus_tag="VGP27_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEV7852276.1"
                     /translation="MKLHETCTTDQAAPQFDHEWQRRAFGLALALSEFRHYPWSEFQE
                     TLIATIGEWESTPESERGQWEYYDHWVAALEKLVDRHELLTAPVLTDAGDHAVAHDHD
                     H"
     gene            7501..7725
                     /locus_tag="VGP27_13145"
     CDS             7501..7725
                     /locus_tag="VGP27_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEV7852277.1"
                     /translation="MSNANPALAIPRVNLRAAAASLSTPARLTAITMLALVAYYFVGF
                     DQGAVSVFGSDTHIHEFLHDGRHLLGFPCH"
BASE COUNT         1375 a         2657 c         2459 g         1350 t
ORIGIN      
        1 ggcgacgagt attctcaacc gttcgaagct caaaaggttt cgggcataga gaaattggct
       61 cgcaacacgc gcagttgagg gatttcgaaa attgatgctg atgactgcac ggtgcgacga
      121 gaacgcgctc agccgtgcag gtggtcagcc gcgcgcgctc gttggatcta ctgggtgcac
      181 cagtcacgga ggatgcgcat tcggcccgcc actccacgcg caacgccgaa gactttcacg
      241 ggatcgaagg gttggtcgac atcgtcgccg cctaaggcga gcccgatgtg gcgctatccg
      301 tcaagcatcg cagcaccgcc gcgccacgcg ttgacgacgt cctcgacgcg ctggtgcacc
      361 tgtgctcgcg ccgattcccg gtcgacaccc gtcatcagca gggtgtcgta gtcggtgtcg
      421 atgtggcgga ccgaagcgac gacggcgagg cgtacagcct cgcgatccaa cgcacgcccc
      481 gcggcgcttc gtcccacccg gccgctgccg cgagccgcgg cgtggagtgc gatcgcctca
      541 gcgcggtcga gcggacagcc cgggaactgc tcacggatgg cggcggcgaa ttcactccgg
      601 aattgcacgt cctgatcggc ccggcggcac tcctcgcgtg tcctacggcg agcccgcacg
      661 tcggcatccg agaggcattc ttgcgcggcc tgttcgattg cggccgcctc cgccaggatg
      721 ccctgccgct catagcgttt gcgtcgtgtg ctccagtgca ccacgacggc ggacagtcgg
      781 ctggctttcg tggcccgccg ggtgagtgcg gcatcgccgg aaggcagaaa ctccagatgg
      841 ccgagatcgg cgcaatccag acacagtgcc cccgccttgt ccatgcgaag gaagtcgccg
      901 gtgtcgccac aggacgcgca cgtccacggg ttgtgcggag cgatcacggt aatcatctgc
      961 tggggacggc atggtgggcg ctcgacaaca atctcgggag tctcggatga cgcccactgc
     1021 gtgcggtagg cgctttcggc ctcaaggtca ccgtcgacgg tgaattgcag gtcttggtaa
     1081 tcggcttctg acggcgtaag gccgcgatgt tcggcccagt cctgcaacgc cgcaatggca
     1141 gtcatgactt tgtcgaagtc gaccgccacg caccgatcga gcgaaggaac acggccccgc
     1201 tcccaccggt cgacgttcgg ctgtgcgagc cagcccagac cgacgagcac atcgacgagg
     1261 cggacgtatc gccgttcggt cagcaattcc tccgcgatcc gtgccactcg tgtttcaacg
     1321 cgtgccatag caagaccgaa tttacttccg cggtgtgaca aagtgcaggc aagtagcggt
     1381 cattcgccat gcaaacccag caaggtgcgc agccgctcta cgctcatgcg cccggctttg
     1441 tcttcatcgg gagggaagct gtcgaggagt ttgatcaggt caccgcgccg cgtgggatcg
     1501 tcagccgcct gccgcggcgt gtgaccgtcg agtgcgggta tgggttcgtc gagccacctg
     1561 gtttcgtact cgcggatgaa ctcgtcgagc atcgcggcca cctcgggggc gtcggtgtcg
     1621 agtgcgtcct cggcagccgg cagctgcgac gctagttcgg cggccccccg cgtgtcgcga
     1681 accgggcgtc ggacgtcgtc gagtacacgc atcgtgggat cgagcccaat caacgtggcc
     1741 aacacacgat ccatccgttc cgtgcttttg gtctcgaccc gcagcatgtc gccttcgcgc
     1801 accagtacaa cacgaatctt cggcatgcca cgggtgttga cctgctcgtg ccaccgcggc
     1861 ggctccgcgc cgtcgacccg gtcataggtc tcgtcgagcg aggccgcgat cttgtccgga
     1921 tcagcgacct gcaccgtggc ttcacaaagc accagcggat cgccttcggt gttggtcagc
     1981 gtcgccggtg cgaaacgtcg actcaccatt gagatcaagt cgaccgggtc gggttcggcg
     2041 tcgagtaggt cgatcagcgc atcccgctcg tgcagcgtga ccggttcggc gccgccgaag
     2101 aactgcatac tgtcgccagc gggcactacg cgtgcgcaga tcagctgacc cgccttcagc
     2161 tggcggctgg cggtccgctc gcgtacctcg tgaacgtcgc cagttcgtat gtcgcgcaca
     2221 gtgagaccct cgccgggtac aacggtctct acttcgaaca ccgaccgctc cacaaggtgc
     2281 cattgttcgg ccaacagccg ctcgtcgtca ggcaacagtg acccgcgcac ctgcagaaac
     2341 tccgcaaacg cgccgccctc gaacagcacg acgtccatca ccagcatgtc agccatccct
     2401 gcgtccaagg cctcggcgag gtcgtgagta tgtcggtatc gctcgtagct cacttcggcc
     2461 agcagatctg tccagccgct gaactgtgca tactgaacgg ccttggcgta caaccaattc
     2521 acccgctggg caagcggaag ctgctcgcgg cctagatggc acttcttgta cttgcggccc
     2581 gacccgcacc agcacggctc gttgcggccg aggtcggggc gcggctccgc tcgatgggcc
     2641 tgcaacagct cgaccaacgg gtggcccggt ccggcgtctg cgcggcgcag cagtcccaac
     2701 ccgcgctcga catcgccgcg atcgttggcg acgcgagcca ggtcgagcaa cggcagcggc
     2761 cagtcaggat ccatcgactc ggccgccagc aattcacgct cggcctgatc gatgtcgccc
     2821 acccgctcca gcgccaccgc ccgcagccac cgccacgcca cccgcgccgt gcgtggcgcc
     2881 ctggatccca tcagctcagc gagcaggccc agagccgccg cgctgccgcg gttggcgccg
     2941 acggtctcag cccgcagcag ctcagccaac agtgggtcga caagctgtgc tcccagctct
     3001 tccggcacac cgatagagcc gtggtcggcg aagagcactg cgctttcgtc ggcctcgccc
     3061 gggtcgtccg cttcgatcag gcgttgcgcc ctgtcgtaca actcgatcag cgcggcgagg
     3121 acgaacccgt cgccggcgtt gaggccgtgc cgctcggcaa gcagttcacc ccggcgctcg
     3181 agttgccacc gcacaatgtc gaatccgcgg ggcgcgagcc actcgccaag gtgcgcgata
     3241 ccgacgtcct cgacgatctc gcttagcggc ggcagtgggt cggtgaacag tgtggggtca
     3301 gcgacgcagg cggtccagac ggccgcgtcg acgtaagtcg gtacgtccgg gtcgaaacgt
     3361 agactcaacg ttgagatcaa gtcgaccgga ctcgaccacg gcgcgttgct ctacgaccac
     3421 cgtgggctcc cgaggtgatc gccgatgcca acgtgcgaag gctctctgcc gcaagctatc
     3481 tcgagtgcgg gatagtcctg gataaccaac gtgacccggt cgtcagcagg agcctcgacg
     3541 agctgctcgc ggaagccgag ttcgtgatcg agccggtgac cgaacatcgg gcccgtctgg
     3601 ctcgccaggc ctatgcagac ttcggtacag aaagcggtca ccgcgccgga ttgaacttcg
     3661 gggattgcct ttcctacgcg ctggcgctcg acacccggga gccgctgctc tggaaaggcg
     3721 acgatttcgt gcacacgggc atagcgtccg cgctcgatca gtagaaaacc cgctcctcga
     3781 agccggcctc aagcgcaatc cggcgggggc tcgagcgaaa cgcgttctgg cgcaagacat
     3841 cagcaggttg cgggccggca acttctacgt cgccaacgaa ggcgaggcct tccacgaatc
     3901 gtcgcgccgt agtacctgac ataccacccg cctacacgct ttgatgaatt ccggtcgtgc
     3961 ggaatcgact gcggtacatg ctgggggaga cgccgagggc gcgacggaag gtgcgtcgca
     4021 tggtgtctgc cgacccgaag cccgctcgtc gcgcaacggc ttcctgaccg tggtctcctg
     4081 tcgcaagtaa gaccttcgct gcctccagcc gcgcctgctc gacgaaccgc gccggcgtca
     4141 tcccgacctg cacccggaac atgcgcacca gatgccgctc gctcaccgcc gcgcgacccg
     4201 ccatcgtcgc gatcgagtgg tcggccgcgg ggtccgcgac caccgagtcc aaaatctcgc
     4261 gcagcccctg agataccggc agtgctgact ccgtccacac actgaactgc gcctgaccgc
     4321 ccgggcgctg caaaaacacc accatccacc gtgctacccg ccgcgccacg tccggcccgt
     4381 agtcgctttc caccatcgcg aggcccaggt cgatgccggc actgattccc gcgccggtga
     4441 tgaaccgtcc gtcctgcacg aacaacgagt ccgggtcgac ctcaacatcg ggataggtac
     4501 gcgctaactc ccggcaatgg gcccaatgcg tcgtcgcgcg acggccgtcg agcaatccga
     4561 gtgcggcgag tacgaatgcg ccggtgcata ccgaggcgac tctgcgcgcc cgcggcgcca
     4621 gttcgcccac catctccagc gcggccgaga cgacgtccgg cgtctgctct tcagggatgt
     4681 catgctcgcc gggcgtgaaa ttgaagtcgg gcggtactcc gcccggcacg atcaacgtgt
     4741 cgatcgggtc cggcacctcc gcaagcggca tatcgacctt gagcgcgacg aacgacgtcg
     4801 tattgacggc ctcacccgtc ggcgacgcca agatcacctg gtatttcgcg ccaaagtcat
     4861 tggcacgggc gaagatttcc agcggcgcgg ccacgtcctg caacgtcacc ttgttgtaca
     4921 acgcgaagac gacgatcttg ggggtcttcg gtcctttggc gcttgcggat cggcgcaccg
     4981 ccgcctgggc cggccgaggc ggtctcttct cgtcggacac ctttgctgcc ctttcgtgac
     5041 cccatctgat gagagagcca agttagcgcg catctgtgat ggcggcagcg cgaacgggcg
     5101 cggaccagaa agcgacgtcc ggaactgcgc gtttcgtgtc cggtcctgac ggtcgaagaa
     5161 atccaggtgt aacgcggtcg aaacctgctg acgcgactct gatttcaccg tcctccgctc
     5221 cggtgccgaa gcgaaggcaa actcccgtct tcggggaagg tgaggccatg gattctcgga
     5281 cagccttgac gctcagcatc ggcgttctcg gcgggctggc ggtcgcgctg accgccgaag
     5341 tcatcacggt gcccatctgg gtggtgttcc tgtcgtgggc gtcgttcttc ttcgtcggcg
     5401 gcggtgtcgc cggctgggtc cggtcggtga cgtcgaacat cgtcggcgtg gtgatcgcct
     5461 cggcgagcct gtatgccgcc tacctgatgg gcggcagcct gctggtcacg gccatcgccg
     5521 tgggcgtcgg cagcgcgttg atggtgcagg cgtcgtgggt gccgctgctg gcgacgacac
     5581 ctgccgtcgt cgtcggcttc gcctctaccg tgtcgacggt cgccggaacc ggtagcgaca
     5641 tcactgtcac gaccatctcc cacccgggcc tggtcgccgc ggtcgcctgt gtgctcgggg
     5701 cgtcgttcgg attgctctcc gaatacgtcg ccaccgcaat gacgaagaaa tcggccgacc
     5761 aaccggccgc cggcgcggag agcccgtcaa ccgaaggaag ccctgcctga tgaaactgca
     5821 gcactatctt ggcggcctcg agggccttcc cgaaccgttg acccttgaag agcgcgtctt
     5881 cgtcgaggag tgggagaaac gcatcttcgg catccacgtc gcgatgatgg gcttgtcgaa
     5941 ccacctcggg tcggcgctgc cggcctaccc catcgacgag gtgccgacgg ccttcaatga
     6001 cgaatggacg tgggccgacc tgcgcaccgg cgccgaggcg atgaacccct tcgactactt
     6061 caagttccgc tactacgaga agtggttggg cgggatcacc cagttcttcg tcgacaaggg
     6121 ctatgtgacc gaagaggaac tcgccgaccg aatggccgac ctcccgccgg cagcagatgc
     6181 cgcgggcgca gacacgatcg atgatcaggt gatcgcctat ctgcgttcgg gtgacagccc
     6241 ccggcgcgat gtcgcacacc ccaagttcgc cgtcggctca ggtgttcgca tcaccaacgt
     6301 gcctgccgac gcacacagtc ggttgccggg ttacctgcgc gggcgcatcg gcacggtgga
     6361 acgcgttttc gaaggtgact acgcgtactt ctgccacacc ggcgacggca tcggggaccc
     6421 catgccgatc tacatcgtcg aattcgagcc ggcggaaatc tggggcgtgc gcgccgagga
     6481 tgggccgctg tcgctgtatg ccgagctttt cgaagcctat ttgcaaccca tggaggagaa
     6541 gtcatgaccg gccagttcgc ctacccgccc gaccgcgagg aggccagcgc caagaaggcg
     6601 gccgcgctgg agtctctcct catcgagaag ggcgtcatca caccccagac cgtggacaag
     6661 gtgctcgcct atttcgagac cgagatgacg ccgctcaacg gcaagaaaat cgtggtgaag
     6721 gcttggaccg atccggaatt cgccgagaag gtcgtcgtcg acacaccggc ggccatcgcc
     6781 gaactcgacc tccccgacgg catggccggc gccgaaggcg agcacctgca ggccgtcgcc
     6841 aacaaccccg gagtgcacaa cctggtgatc tgcaccctgt gctcctgctt cccgtggccg
     6901 gtgctcggac ttccgcccta ctggtacaag gacccgacct tccgttcgcg cgccgcccgc
     6961 gaaccgcgca aggtgctcgc cgaagtcggc ctcgatctac cggccgacac cgagatcaag
     7021 gtatgggatt ccagcggtca ctcacgctgg ttcgtcatcc ccgaaaggcc caccggcacc
     7081 gatgatttca ccgacgagat gttgatgaac ctggtgacca ccgaggcgat gatcggtgtg
     7141 gcgcaggccg ggccggtatc atgaaactgc acgagacctg caccaccgac caggcggcgc
     7201 cgcagttcga ccacgaatgg cagcgcagag ccttcgggct ggcgctcgcg ctctccgagt
     7261 tccgccacta tccctggagc gaattccagg aaaccctgat cgcgacgatc ggcgagtggg
     7321 agagcacgcc cgaatccgaa cggggacagt gggagtacta cgaccactgg gtcgcggcgc
     7381 tcgagaagct tgtcgaccgc cacgaactgt tgaccgcccc ggtgctcacc gatgcaggtg
     7441 accacgcggt tgcacacgac cacgaccact agcccgacat gacaccgtga ggatttccac
     7501 atgagcaatg ccaaccccgc cctcgccatc ccgcgcgtga acctgcgggc ggccgccgcg
     7561 tctctttcca ccccggcccg gctgaccgcg atcacgatgc tggccttggt cgcgtactac
     7621 ttcgtcggct tcgaccaagg cgcagtgtcg gtgttcggtt cggacaccca catccacgag
     7681 ttcttgcacg acggccgaca cctcctggga ttcccatgcc attgagcacc gaaataccct
     7741 ctgtcgtccg atatctcgtt cccggtgcgg tagccggcat agtgtccgcc atcttcagcc
     7801 gagtgatgat agagccgttg atcggcgccg cggtggacta c
//
LOCUS       DASYLX010000543         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2439616,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000543 DASYLX010000000
VERSION     DASYLX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439616"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VGP27_13150"
     CDS             <1..153
                     /locus_tag="VGP27_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEV7852278.1"
                     /translation="GGYVTYRVFFADEADAVAAGYRPCARCMPEKYRAWKDFRRLRTT
                     PVDAKP"
     gene            complement(128..748)
                     /locus_tag="VGP27_13155"
     CDS             complement(128..748)
                     /locus_tag="VGP27_13155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852279.1"
                     /translation="MNQTKAHWEEHYRERERVWSGRVNVQFADVVADLAPGRALDLGC
                     GEGGDAVWLAERGWHVVAVDISDTALGRAREAADSRGVADRIEFVQLDLSDSFPNGTF
                     DLVSSQFLHSTVQLDRTKILVNAAAALRSGGLLVIVDHGSAPPWVSKLDHHHEFPSAE
                     EVIAGLDLSADEFERLRVEAVERQASGPEGQQRPWMDNVMVLRRLA"
     gene            795..1376
                     /locus_tag="VGP27_13160"
     CDS             795..1376
                     /locus_tag="VGP27_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV7852280.1"
                     /translation="MQAKGTSEDDVDLRVRRRLRELRVERGLTLEDVARSARIDVSTL
                     SRLESGKRRLALDHLPRLAEALSVSTDELLRVPEAPDPRVRGSSHTHNDITYWPLTRQ
                     AAAGGLHAFKTRIAARRRKPPAELPVHEGQEWTYVLSGRMRLILGERDFVIEPGEAVE
                     FSTWTPHWFGAVDGPVESIVIFGLHGERLHLHE"
     gene            complement(1377..2477)
                     /locus_tag="VGP27_13165"
     CDS             complement(1377..2477)
                     /locus_tag="VGP27_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RNase H/acid phosphatase"
                     /protein_id="HEV7852281.1"
                     /translation="MKVIVEADGGSRGNPGPAGYGSVVWTADHKTVLAESKQSIGHAT
                     NNVAEYRGLIAGLEAAVKVGATEVDVRMDSKLVVEQMSGRWQVKHPDLKVLNKQAAAV
                     ATKFERIGYTWIPRAENSYADRLANEAMDAAAELETGSGEAEEIGKQPQTPASPAGWT
                     GARGAPTRFLLLRHGQTELSAQRRYSGRGNPALTEVGLRQAEAAAQYLGQRGGIAAVI
                     TSPLQRAYDTAAKAAKALGLDVIVDNDLIETDFGEWEGLTFNEVAERDPDLHRRWLRD
                     TSINPPDGESFDSVSDRVQRARGRIISEYAGDTVLVVSHVTPIKTLLRLALDAGPSIL
                     YRLHLDLASLSIAEFYPDGASSVRLVNETSYL"
     gene            complement(2474..>2592)
                     /locus_tag="VGP27_13170"
     CDS             complement(2474..>2592)
                     /locus_tag="VGP27_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEV7852282.1"
                     /translation="RIEIDRGEIARITAAADDDVLRCPECGAILLRVKDGGQ"
BASE COUNT          438 a          905 c          803 g          446 t
ORIGIN      
        1 ggcggctatg tcacgtaccg cgtgttcttc gccgacgagg ccgacgccgt cgcggccggc
       61 taccggccct gcgcccgttg catgcccgag aagtaccgcg cctggaagga ctttcgacgt
      121 ctgaggacta cgccagtcga cgcaaaacca tgacgttgtc catccagggg cgctgctggc
      181 cttccgggcc cgacgcctgc cgttcgacgg cctcgacgcg gagccgttcg aattcgtcgg
      241 cactgagatc caagccggcg atcacctctt cggcgctggg gaattcgtga tgatggtcaa
      301 gcttggagac ccacggcggc gccgaaccgt gatcaacgat cacgagcagt cctcccgacc
      361 gcagcgcggc ggccgcgttc acgaggattt tcgtccggtc cagctgaacg gtcgaatgca
      421 ggaactgcga cgagacgagg tcgaacgtgc cgttggggaa actgtcggac aaatcgagtt
      481 gcacgaactc gatgcggtcg gcgacgccac gcgagtccgc cgcctccctg gcgcgaccga
      541 gggcggtgtc agagatgtcg accgcgacga cgtgccagcc tcgttcggcg agccagacgg
      601 cgtcaccacc ctcgccacag ccgagatcga gcgcgcggcc cggcgctaga tcggctacca
      661 cgtcggcgaa ttggacgttg acgcgtccac tccacactcg ttcgcgttct cggtagtgct
      721 cttcccagtg cgccttcgtt tggttcatgc gaccagcttg cacccaggtg cacgatctgc
      781 caatctgatt tgccatgcag gcaaagggca cctccgagga tgacgtcgac ctacgggttc
      841 gacggcgact gcgggaactt cgcgttgagc gcggcctgac gctggaggac gtggcccgca
      901 gcgcccgcat cgacgtgtcg acgctcagtc ggctcgagtc gggcaagcgt cggctggcac
      961 tggaccatct cccccgcctc gccgaggcgc tgtcggtcag caccgatgaa ctgctgcgcg
     1021 tacccgaggc gcccgatccc cgggtgcggg gcagctcgca cacccataac gacatcacct
     1081 actggccgct cacccgccag gcagccgcgg gcggcctaca cgccttcaag acccggatcg
     1141 ccgcaagacg ccggaaaccg ccggctgagc tacccgttca cgaaggccag gagtggacat
     1201 acgtgctctc cggccgaatg cgactcatcc tcggcgagcg cgacttcgtc atagaaccgg
     1261 gcgaagccgt cgagttcagc acatggaccc cgcactggtt cggcgccgtg gacggaccgg
     1321 tcgaatccat cgtgatcttc ggcctgcacg gcgaacgcct acacctgcac gagtgactac
     1381 agatacgacg tctcgttcac caaccgcacg gatgacgcac cgtcggggta gaactcggcg
     1441 atcgacagcg atgccagatc caggtgcaac cggtacagga tgctcggccc cgcatccaat
     1501 gccaaccgca gcagcgtctt gatcggcgtc acgtgcgaca ccacaagcac tgtgtcgccc
     1561 gcatattcag agatgatccg accgcgagcc cgctgcaccc gatccgagac actgtcaaag
     1621 ctctcgccgt ccggcggatt aatgctggtg tcgcgcaacc aacgccggtg caagtccggg
     1681 tcgcgttcgg cgacctcgtt gaaggtcagg ccctcccact cgccgaagtc cgtctcgatc
     1741 aagtcgttgt cgacgatgac gtcgagaccc agtgccttgg cggccttcgc cgcggtgtcg
     1801 taagcgcgct gtagcggcga cgttatgacc gccgcgatac cgccccgctg cccgaggtat
     1861 tgcgccgccg cctccgcctg tcgcaggccg acttcggtga gtgccggatt tcctcggccc
     1921 gaataccgtc gctgcgcaga taattcggtc tgcccgtgcc gcagcaacag gaaacgtgtc
     1981 ggagcgccgc gcgcgccggt ccagcctgcg ggcgaggccg gtgtctgcgg ctgcttaccg
     2041 atttcctcgg cttcgccgga tccagtctcc agttcagcgg ccgcatccat cgcctcgttg
     2101 gccagccgat cggcatacga gttctcggcc cgggggatcc acgtgtagcc gatgcgctcg
     2161 aacttcgtcg ccaccgccgc cgcctgcttg ttgagtacct tgaggtcggg gtgcttgacc
     2221 tgccagcggc ccgacatctg ctcgaccacc agcttcgagt ccatccgcac gtccacctcg
     2281 gtggcgccga ccttcaccgc agcttccaga cccgcgatca gaccgcggta ttcggcgacg
     2341 ttgttggtgg catggccgat cgactgctta ctctcggcca gcacggtctt gtggtcggcg
     2401 gtccacacca ccgagccgta tcccgcagga ccgggattgc ctcgggaccc accgtcggcc
     2461 tcgacgatga ccttcactgg ccgccgtctt tgacgcgcaa caggattgca ccacactcag
     2521 ggcagcgcaa tacgtcgtcg tcggcggcgg cggtgatccg cgcgatctcg ccgcgatcga
     2581 tctcgatgcg gc
//
LOCUS       DASYLX010000544         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3206938,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000544 DASYLX010000000
VERSION     DASYLX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206938"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /gene="moaA"
                     /locus_tag="VGP27_13175"
     CDS             <1..916
                     /gene="moaA"
                     /locus_tag="VGP27_13175"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV7852283.1"
                     /translation="CTYCMPAEGLDWMPDDQLLTVDELTRLMRIAVTRLGITRVRFTG
                     GEPLLAKRLEHVVAATADLRPRPEITLTTNGLGLDRRAAGLKRAGLDRINVSLDTVDA
                     DHFTHITRRNRLPDVLAGLAAAHRAGLRPVKVNAVLDPATGLGDAVSLLRFCLDHDYQ
                     LRIIEQMPLDAGHQWSRGAALGADDILAGLRHHFALAPDPAPRGSAPAQLWLVDGGPA
                     TVGIIASVSHAFCGDCDRTRLTADGQIRSCLFSREETDLRGLMRAGAGDDVVEAAWRA
                     AMWAKPAGHGINDPGFVQPDRPMSAIGG"
     gene            916..1212
                     /locus_tag="VGP27_13180"
     CDS             916..1212
                     /locus_tag="VGP27_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV7852284.1"
                     /translation="MHTQRARQTSVETTVRVRLTVRYFAAARAAAGAEAETVLIAPGT
                     TVAALVETLSVRGPELAQVLRRCSFLCDGVAVRDEGLNLSDGQTIDVLPPFAGG"
     regulatory      1428..>1639
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          280 a          575 c          531 g          253 t
ORIGIN      
        1 gtgcacgtat tgcatgcccg ccgagggcct cgactggatg ccggacgatc agctgctgac
       61 cgtcgacgag ctgacccgtt tgatgcgcat cgcggtgacc cggctgggaa tcacccgcgt
      121 tcgcttcacc ggtggcgaac cgctgctcgc caagcggctc gaacacgtcg ttgcggccac
      181 cgctgacctg cggcctcgac cggagatcac gttgaccacc aacggccttg ggctcgatcg
      241 ccgcgcggcg ggcctgaagc gggcgggcct ggatcgcatc aacgtctcgc tcgacaccgt
      301 cgacgccgac cacttcaccc acatcacccg tcgcaaccgg ctgccagatg tccttgccgg
      361 gctggccgcg gcgcatcggg ccggcctgcg gccggtgaag gtgaacgccg tgctcgaccc
      421 ggcgacgggc ctcggcgacg cggtgtcact gctgcggttc tgcctcgacc acgactatca
      481 gctgcgcatc atcgagcaga tgccgctcga cgccggccac cagtggtcgc gcggcgcggc
      541 gctgggcgcc gacgacattc tcgccggcct gcgacaccac ttcgctctgg cgcccgatcc
      601 ggcgccgcgc ggttctgctc ccgcgcagct gtggctggtc gacggtgggc ccgcgaccgt
      661 cggcatcatc gcgtcggtgt cgcacgcgtt ctgcggcgac tgtgaccgga cccggctgac
      721 cgcagacggt cagatccgca gctgtctgtt ctcccgcgag gagaccgacc tgcgcgggct
      781 gatgcgcgca ggcgccggcg acgacgtcgt cgaggcggcc tggcgcgcag cgatgtgggc
      841 caagcccgcg ggccacggca tcaacgaccc cggcttcgtc cagcccgacc gcccgatgag
      901 cgcgatcggg ggttgatgca cacgcagcga gcacgacaga catcggtgga gaccacggtg
      961 cgggtccggc tcaccgtccg ctacttcgcc gcggcccgcg cggcggccgg cgcggaggcc
     1021 gagaccgtgc tgatcgcgcc gggaacaacc gtcgccgcgc tcgtcgaaac gctgtccgtg
     1081 cgcggccccg agcttgccca ggtactacgt cgatgctcgt tcctgtgcga cggagtcgcc
     1141 gtgcgcgacg agggcctaaa cctctccgac ggccagacaa tcgatgtgct tccgcccttt
     1201 gccggcggat aaaagtgatt tgcatcacat aacgaaatgg tcacaagctg gctacggacc
     1261 ggtgaccctc ggtatacacc agcggaaacg cccggaattc ctgcgctttt agagcctcat
     1321 taggatttgc cgccgggaga aacgccgtac cagccaaaaa atgactacaa cgctgcgaac
     1381 ggttatgttc ttccgcaagc tggtcgaccc taatcgtcta gccctttctg tccgaagcgc
     1441 cgagctccat ccgtcggaca ggaaaacgac aaacaactgg gatggaggcg ggggacccac
     1501 cggtccaccg agatagaagg accaggaacc gcaagcggtt ccttggggtg aagccgaaac
     1561 cgttctcacc gacggccagg ccgggcaacc tctccagccc gaacccgaca gctgacctcg
     1621 cgggcgcatc agagaggat
//
LOCUS       DASYLX010000545         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3206940,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000545 DASYLX010000000
VERSION     DASYLX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206940"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="VGP27_13185"
     CDS             complement(<1..1685)
                     /locus_tag="VGP27_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEV7852285.1"
                     /translation="MLLDGNSLAFRAFYALPAENFKTQGGLTTNAVYGFTAMLINLLR
                     DEQPSHIAAAFDVSRQTFRLERYPEYKAGRSATPDEFRGQIDITKEVLGALGITVLAE
                     PGYEADDVIATLATQAENEGYRVLVVSGDRDSLQLVSGDVTVLYPRKGVSELTRFTPE
                     AVLEKYGLTPLQYPDFAALRGDPSDNLPGIPGVGEKTATKWIAEYGSLQALVDQVDTV
                     KGKVGDALRANLSHVVLNRELTDLVKDVPLAQTPDTLRMQPWDRDQIHRLFDDLEFRV
                     LRDRLFDTLASADPEVDQGFDVRGGALEAGELGAWLSEHTAGKRSGLAVIGTHLAFDA
                     DATALAIVAGDGDGRYLDTTDLDPDDEAALASWLCDVESPKALHEAKVAIHDLEGRGW
                     TLRGVTSDTALAAYLVRPGQRSFTLDDLSLRYLKRELRADNPEQQQLSLLDDSDGVDD
                     QAVQTSILRASAVMDLADALDEELARIESSSLLGDMELPVQRVLAEMETAGIGVDLGL
                     LSELQSEFADQIRDAAEAAYGVIGKQINLGSPKQLQVVLFDELEMPKTKRTKTG"
     gene            complement(1743..2633)
                     /locus_tag="VGP27_13190"
     CDS             complement(1743..2633)
                     /locus_tag="VGP27_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7852286.1"
                     /translation="MELRQLEYLVAVAEEANFTRAAERIHVAQPAVSAQIQRLERELG
                     QALLDRSRRAVRLTAAGEAVLPHARAALAAVQNMHVAADELSQLVRGAVTIGTVTSHN
                     VDIPLLLADFHADHPNVEITLSTDTSDNLIDDVRSGKLDAAIASVGPDEVPEGLGVEV
                     ATDEAIEAAVCRTDPLAKRKKVRLADLHDRTLIALPIGAGIRHQFDRACAGAGLTPRI
                     AFEASTPLALADLAAQGLGVAIVPESVPRGRDDLQSLPFVPELRGRLVLAWRSEGPIS
                     PAARVLIDRARRLLRVSDRA"
     gene            2704..3846
                     /locus_tag="VGP27_13195"
     CDS             2704..3846
                     /locus_tag="VGP27_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7852287.1"
                     /translation="MTENVLVAGAGIGGLTAALALDAKGIDVTVVECTDLRPLGVGIN
                     LLPHAVRELHALGLGEELSRIAVAPAAISYYDTDGTLLFREPRGVDGGYGCPQYSVHR
                     GRLQALLLSSLRDRRGPGVVRDSTEVAAFEESADGVHVQTSAGEFAADVLVGADGIHS
                     TVRRVLHPGPDPLLWSGLRMFRGAVDGDPFLDGRTMSIVKSADNIDLVVYPIGNDQIN
                     WVVMLPGGDPGPLPGDARWSQPGNPSEVLHHLEQWNLGWLDAADLVRRTDPVLEYPMV
                     DRDVLPYWGRGRVTLLGDAAHPMYPVGANGGSQAIVDAAVLAQQLSRDFENGLRRYEH
                     RRRPETAQVVKANRDMHRAGTTRRSDELARVTARYRTDTNADRSRS"
     gene            3843..4517
                     /locus_tag="VGP27_13200"
     CDS             3843..4517
                     /locus_tag="VGP27_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7852288.1"
                     /translation="MTTYVLIPGACHGAWCFDDVAAALREEGHRVLSFTLTGVAERAH
                     LAHAGVNLETHITDVLAALATESDIEDLVLVGHSYGGMVISAVADRIPEKVDALVYLD
                     AFVPRDGESCWHLVNDEQRAWYLDVDDTGYGVPPMPFFDPRASAHPLASLLQRITLTA
                     DMNRFRRRDFVYALNWPGDSPLRPSYERVKDDPNWTVHELDGAHNLMRDNPDDLVRIL
                     LDVGRR"
     gene            4584..>4812
                     /locus_tag="VGP27_13205"
     CDS             4584..>4812
                     /locus_tag="VGP27_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852289.1"
                     /translation="MTEERAELLPDGHRLTHPLHEPSRSDRPRHSQDHFEVSNWNTFQ
                     FCRRSRYADRVSASTQPAIDGWFATDGSGQAY"
BASE COUNT          781 a         1630 c         1562 g          839 t
ORIGIN      
        1 ccggtcttgg tgcgcttggt cttcggcatc tcgagctcgt cgaacaacac cacctgcagc
       61 tgtttgggcg agccgaggtt gatctgcttg ccgatgactc cgtaagccgc ttcggcggcg
      121 tcccggatct ggtcggcgaa ctcgctctgc agctccgaga gcaggccgag gtccacgccg
      181 atgcccgccg tctccatctc ggccagcacg cgttgcaccg gcagctccat gtcgccgagc
      241 agagacgagg actcgatacg cgccagctcc tcgtcgagag catcggcgag atccatcacc
      301 gcactggccc gcaggatgga tgtctggacc gcctggtcgt ccaccccgtc gctgtcgtcg
      361 agcagcgaaa gctgctgctg ttcagggtta tccgcgcgca gttcccgctt cagatatcgc
      421 aacgacagat cgtcgagcgt gaaactgcgc tggccggggc ggacgagata tgcggccagc
      481 gcggtgtccg acgtgacacc gcgcaacgtc cagccgcggc cctccaggtc gtggatggcg
      541 accttcgctt cgtgcagcgc cttcggcgac tccacatcgc agagccacga cgcgagcgcc
      601 gcctcgtcgt cggggtccag gtccgtcgtg tcgaggtatc ggccgtcgcc gtctcctgcg
      661 acgatcgcca gcgccgtcgc gtccgcgtcg aaagcgaggt gggttccgat cactgccaaa
      721 ccggaccgct tgcccgcggt gtgctcggag agccaggcgc ccagctcgcc cgcttcaagc
      781 gcgcctccgc gcacgtcgaa accctggtcg acctccggat cggccgacgc cagcgtgtcg
      841 aacaaccgat cccgcagcac ccggaactcg agatcgtcga acagccggtg gatctggtcg
      901 cgatcccagg gctgcatgcg cagcgtgtcg ggggtctgcg ccagcggcac gtccttgacg
      961 agatcggtga gctcacggtt gagcacgacg tgcgacagat tggcccgcag cgcgtcgccg
     1021 accttgccct tcacggtgtc gacctggtcg acgagggcct gcagcgaacc gtattcggcg
     1081 atccatttcg tcgcggtctt ctcccccacc ccggggatgc cgggcaggtt gtcgctgggg
     1141 tcgccgcgca gcgccgcgaa atccggatac tgcagtgggg tcagcccgta cttctccagt
     1201 accgcctccg gcgtgaatcg ggtcagctcg ctgacgccct tgcgcggata cagcaccgtg
     1261 acgtcgccac tgaccagttg cagcgagtcg cggtcgccgc tgaccacaag cacccggtag
     1321 ccctcgttct cggcctgcgt cgccagcgtg gcaatcacat cgtcggcctc gtagccgggc
     1381 tcggccagca cggtgatacc cagcgcaccg agcacctctt tggtgatgtc gatctgaccg
     1441 cggaactcat cgggggtggc agaccggccc gccttgtact cggggtatct ctccaaccgg
     1501 aacgtttggc gggacacgtc gaacgcggcg gcgatatggc tgggctgctc gtcgcgcagc
     1561 agattgatga gcatcgcggt gaacccgtac accgcgttgg tggtcagacc gccctgggtc
     1621 ttgaagttct cggcgggcaa tgcgtaaaac gcccggaacg ccagcgaatt gccgtccaac
     1681 agcatcaatg tcggcttttc ggtcaccttc tcggccgttg cgcttgcggt cacggcccta
     1741 ctctatgcac ggtcgctgac acgcagtagc cgacgagccc tatcgatcag aacacgggct
     1801 gccgggctga tcggcccttc cgagcgccac gccagcacca gccgaccgcg cagttccgga
     1861 acgaacggca acgactgcag gtcgtcgcga cctcgcggca ccgactccgg cacgatcgcg
     1921 acgccgaggc cttgtgcggc cagatccgcg agggccagcg gggtgctggc ctcgaatgcg
     1981 atccgcgggg tgaggccggc cccggcacag gcgcgatcga actgatggcg aatgcccgct
     2041 ccgatcggca atgcgatgag cgtccggtcg tgtaaatcgg ccaggcggac cttcttccgc
     2101 ttggcgagcg gatccgtgcg gcacaccgcg gcttcgatcg cctcgtcggt ggcgacctcg
     2161 acaccgagtc cctcagggac ctcgtccgga ccgaccgagg cgatcgcggc atccagcttt
     2221 ccagaccgaa catcgtcgat gaggttgtcg gacgtgtcgg tgctcagcgt gatctcgaca
     2281 ttggggtggt cggcgtgaaa gtcggccagc agtagcggga tatcgacatt gtgtgatgtc
     2341 accgtgccga tggtgacggc gccccgcacc aactggctga gttcgtccgc ggcgacatgc
     2401 atgttctgca ccgccgccaa ggcggcccgc gcatgcggca gtaccgcttc gccggctgcc
     2461 gtgagccgca ccgcccggcg agaccggtcg agcagcgcct gtccgagttc gcgttccagc
     2521 cgctggatct gtgcgctcac cgcgggctga gcgacgtgga tgcgctctgc cgccctggtg
     2581 aagttggcct cctcggcaac ggcaaccagg tactccagct gccgaagttc cataagcaat
     2641 cattatagtt tgacgaataa atccgtattg gacttatgga aaggatcggc ccaatctggg
     2701 gatatgaccg agaacgttct cgtcgcgggg gcgggaatcg gcggactgac cgccgccctt
     2761 gcgctagacg ccaaaggtat cgacgtgacc gtcgtcgaat gcacggacct tcgtccgctg
     2821 ggcgtcggta tcaacctatt gccgcacgcc gttcgcgagc ttcacgcgct cggactcggc
     2881 gaagaattgt cgcggatcgc cgtggctccc gccgccatct cctactacga caccgacggc
     2941 acgcttctgt tccgggaacc gcgcggagtt gacgggggct atggctgtcc gcagtactcc
     3001 gtgcaccgtg gacgactgca ggctctgttg ctgtcgtcgc tacgggaccg tcgaggcccc
     3061 ggcgttgttc gtgacagcac ggaggtggcg gccttcgagg agtcggccga cggtgtacac
     3121 gttcagacgt ctgccggtga gttcgccgcc gatgtgttgg tcggcgccga cggcatccac
     3181 tccaccgttc gccgggtgct ccacccggga ccggacccgc tgctgtggtc ggggctgcga
     3241 atgttccgcg gcgcggtcga cggcgacccg ttcctggacg gccgaaccat gtcgatcgtc
     3301 aagagcgccg acaatatcga tctggtcgtg tacccgatcg ggaacgatca gatcaactgg
     3361 gtggtgatgt tgcccggcgg agatccgggg ccgctgcccg gtgacgccag atggagtcag
     3421 ccgggaaacc cttccgaggt gctgcatcac ctcgagcagt ggaatctcgg ctggctcgac
     3481 gcggccgatc tcgtgcgtcg caccgacccg gtgctggagt acccgatggt cgaccgcgac
     3541 gtgcttccgt actggggccg cggccgggtc acgctcctcg gcgacgcggc acacccgatg
     3601 tatccggttg gcgcgaacgg tggttcgcag gcgatcgtcg acgcggcggt gctggcccag
     3661 caactgtcgc gcgacttcga aaacgggcta cgccgctacg aacaccggcg tcgtcccgag
     3721 acggcgcagg tggtgaaggc caaccgcgac atgcaccgcg cagggacaac ccggcgttcc
     3781 gacgagctgg cccgcgtcac tgccaggtac cggaccgata caaacgcaga caggagccgg
     3841 tcatgacgac ctatgtcctc atccccggcg cctgtcacgg cgcttggtgc ttcgacgatg
     3901 tcgcggccgc tctgcgcgag gaaggccacc gggtgttgag cttcacgctc accggcgtcg
     3961 ccgaacgcgc acacctcgcg cacgccggcg tcaatctgga aacccacatc accgacgtgc
     4021 tggccgccct ggcgaccgag tccgatatcg aggatctggt tctggtcggg cacagctacg
     4081 gcggcatggt gatatcggca gtcgccgacc gcatccccga gaaggtcgac gccttggtat
     4141 atctcgacgc gttcgtgccc cgcgacggtg aatcctgctg gcatcttgtc aacgacgagc
     4201 agcgtgcttg gtacctcgat gtcgacgaca ccggctacgg cgtgcctccg atgccgttct
     4261 tcgacccgcg ggccagcgcc catccgcttg cgtcgcttct ccagcggatc acgctgactg
     4321 cagacatgaa ccggttccgg cgtcgcgact tcgtgtatgc gcttaattgg ccgggagatt
     4381 cgccgctgcg accgtcctac gagagggtca aggacgaccc gaactggacc gtccacgaac
     4441 tcgacggcgc gcacaacctg atgcgggaca accccgacga cctggtgcgc atcctgctag
     4501 acgtcgggcg tcgctagatc ggtcgcgctc ccggcccgcc accgccggcg cggcaccggg
     4561 cggtggcggg ccgagttggc tcagtgaccg aggaacgtgc ggaactgctg cccgacggcc
     4621 atcgactgac ccatccgctg cacgagccgt ccagatcgga tcgtccccgc cactcccaag
     4681 atcacttcga ggtgtcgaac tggaacacgt ttcagttctg ccgccgcagt cggtacgctg
     4741 accgcgtgtc ggcttctacc caacctgcga tcgacggctg gttcgccacc gacggctcgg
     4801 gacaggccta tt
//
LOCUS       DASYLX010000546         7253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2330747,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000546 DASYLX010000000
VERSION     DASYLX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330747"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VGP27_13210"
     CDS             <1..1036
                     /locus_tag="VGP27_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852290.1"
                     /translation="CRIPLPQLLDPAGIGHITATVTEIDAAAATVGTADGVIHGYDRL
                     VLAVGSRVAKPDLPGLAEFSYDVDTYDGAMKLQAHIGDLAGREADSAAATAVVVGAGL
                     TGIETASELPVMLSAALGPGITPRVILVDHNPLVGSDMGDSARPVIEKALAANAVETM
                     TGVGVAAVDEKSVTLSSGDVIPARTVVWCAGMRANPLTAQFGLPRDRLGRLPVDDCLR
                     VGGVDGVFAAGDVAVARMDDDHLSVMSCQHGRPMGRYAGYNVVSDLMGAPMLSLRIPW
                     YVTVLDLGPAGAVYTEGWDRKVVSRGEEAKATKRLINGERIYPPLIGGRAGLLAAAAP
                     NLQAPPAYGR"
     gene            complement(1078..2484)
                     /locus_tag="VGP27_13215"
     CDS             complement(1078..2484)
                     /locus_tag="VGP27_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852291.1"
                     /translation="MTATLVQPGRKVGLHDIGRRLDRLPVGRIHRKIVVAIGLGLFFE
                     VYEIFLSGTIATALKTEYGVGGTALQLLMASSFLGMFIGAAAFGRIADRIGRRRAFLL
                     NLVWFSVWSIIGAIAPNPLMLVGARFMAGIGVGAEYPVADSYLSDVLPKDHRGRLAAW
                     AYTCSFLAVPALGFLTLSLNGHKVLGFDSWRVLLVIGAIGALFVIVLRRALPESPRWL
                     AGVGRTEEAEAAMRAFESGTDSNTAEVADVDLDVEDVPEHASEPTHTVNAIARLRLSP
                     YRQRLFMLVVFHLFQPFGYYGFGTLAALVLVSRGYDVTSSLLFTAVSFIGYPLGSLIS
                     IPLLKRFERKYLIMATIAVMAVCGPLFAVVSVPVMIVVFGLLTTAASNVFSNIYHVYQ
                     AEIFPSDVRATAVGWTYSVSRLSSAALPFILIPVLNHYGATAMFGVVLVALVAAVAVL
                     IPIGPRTTGKSLDEINPT"
     gene            complement(2481..3287)
                     /locus_tag="VGP27_13220"
     CDS             complement(2481..3287)
                     /locus_tag="VGP27_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852292.1"
                     /translation="MTTFPSNQNRNWTATQAASWQTSSDVPTMAAYFPPTMAGPTAPV
                     APPHAYAYGPAYGQAPMPALAWSAEQPEFNSVSAAYPAQQPWYARTRVLAFAGAGLVA
                     AAAAGLFGALHSSSSTPVNTAAHNATAPAAAPAPAPAPAPMPAVQPAAPRHRSSSHST
                     MSSGAYSKPASHQAPPQSPPPPPAPSQQPSDQSGHSDHDSQWDNSGNYWWNHRDDNSY
                     RRHGDHDNRWNFWNRDHDSDNSHSSGDHDSSNSSNDHSSDSQSDDNGDSK"
     gene            complement(3470..4843)
                     /locus_tag="VGP27_13225"
     CDS             complement(3470..4843)
                     /locus_tag="VGP27_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7852293.1"
                     /translation="MLMQLDDDCARLRELGYEPELARTLSVRDLVIHGLIYMVPLAPI
                     GVFGILYNLSAGAVAAVYVIAAVAMLFSAISYKEMARVFPVSGSTYSYVSLGTNRFLG
                     FVSGWAILLDYLLLPALLCVFSASAMTTIVPFIPAWTWVVFFVAVTAVTNLLGMNVAA
                     KMNKYSLYIQLAILAVFLCWAASLVLQGQAHLTLDPFHPHGGASWSVVAGAIPVAAFS
                     FLGFDAVSTLNEEAKGGGKAVSKATMLVMYLSTVLFVVQVYFACILVPSGTHYADGTA
                     ANNAFYDLVGNIMDSKFKMFIVLNSALFAALGNIVAANATASRLVYSMARDGHLPKLF
                     ARVSGKRKVPVNALVIICALALAIGVFGVGQASLLTTLVTFGALLSYILMHFSVITHF
                     FKRNGSRRWFTHVISPLLGAAILMVALWDADTNAKVVGIIWLIIGLGVATYMRLSGRD
                     LKVSETA"
     gene            5008..6696
                     /locus_tag="VGP27_13230"
     CDS             5008..6696
                     /locus_tag="VGP27_13230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7852294.1"
                     /translation="MVRMRPVDVRLATQVLVLQVAVVALTLGLAGGMLAFFSHDRLAS
                     QYEDQSLDVARAVAFAPAVRADVTRYDDAPLTPSPALTAELAGGPLQTLATEIQKHAN
                     LLFVVITNDTGIRLSHPDRNELGKHVSTDPSEALAGHEVVDRESGTLGSSVRAKVPIY
                     APNSTRVVGEVSVGISTETVHHQLWSDVRRAAVLVGVALLIGVVGSVLLARRWRGLTL
                     GLQPSEMAELIRGQAAVLHGIDEGVLAVDTEWKTTFVNDLACRMLGIDDETGRPVEEI
                     GLTPRVLEVFKSAESTPTLATVGDRIVLVSARRVAREGRELGTVLVVRDRTDVESLTR
                     QLDAVQLMSTVLRAQRHEFANRLHLLNGLLHSGHVEEAGQYVEELLGSGPLGSAIPGI
                     DAIRDAFLQAFLAAKSASARESGVTLTIGENTWVPGRLALPVDVTTVLGNLLDNAIYA
                     ARTGDSDAKVVEAELLQDGSTLHITVVDTGDGVPPNFVDHLFTEGKSTKPDSGIPGGR
                     GIGMALSRQIIRALDGEIRLSSPGNPGAHLCGAEFIARLPGVMVEEEAQWAAQN"
     gene            6678..>7253
                     /locus_tag="VGP27_13235"
     CDS             6678..>7253
                     /locus_tag="VGP27_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7852295.1"
                     /translation="MGRSELTVLVVDDDFRVANMHAGIVNALPGFTVTTTATTLSAAR
                     KADLVDLALVDVYLPDGSGVDFVRELRCDSMVLSAATDAPTIRAAIAAGALSYIVKPF
                     APADLAARLGGYARYRKLLAGTNLGRKEVDAALDALRPKIAPVQSPTAVASPTKDLVL
                     QALRASGQPMSSAEVSTEIGVSRATAQRYLST"
BASE COUNT         1295 a         2299 c         2445 g         1214 t
ORIGIN      
        1 ctgccgaata cccttgccgc agctgctcga tccggccgga atcggccaca tcaccgccac
       61 ggtgaccgag atcgacgctg cggccgcgac cgtgggtacg gccgacggtg tgattcacgg
      121 ctatgaccgc ctcgtgctcg ccgtgggcag cagggtggcc aaaccggacc tgcccggact
      181 ggccgagttc agctacgacg tcgacaccta cgacggtgcg atgaagctgc aggcgcacat
      241 tggcgacctg gccggccgcg aggcagattc cgcagccgcg acagcggtcg tcgtgggcgc
      301 cggtctcacc ggaatcgaaa ccgccagcga gctgcccgtg atgttgtcgg ccgcgctggg
      361 acccggcatc acaccgcggg tcatcctcgt cgaccacaac ccgctcgtcg gatcggacat
      421 gggtgactct gcccggcccg tcatcgagaa agcgttggcc gcgaacgcgg tcgagacgat
      481 gaccggagtt ggcgtcgccg ccgtcgatga aaaaagcgtg acgttgtcgt ccggtgacgt
      541 catccctgcc aggacggtgg tgtggtgcgc ggggatgcgg gccaacccgt tgaccgcgca
      601 attcggcctg ccgcgtgacc ggctgggccg gctaccggtc gacgactgtc tacgggtcgg
      661 gggcgtcgac ggtgtgttcg cggcgggcga cgtggcggtg gcgaggatgg acgacgacca
      721 cctgtcggtc atgtcgtgtc agcacggcag gccgatgggc cgctatgccg gctacaacgt
      781 ggtcagcgac ctgatgggtg ctcccatgct gtcgcttcgc atcccctggt acgtcaccgt
      841 tctggatctc ggccctgccg gtgcggtgta taccgagggc tgggaccgca aggtcgtcag
      901 ccgcggcgaa gaggccaagg ccaccaagcg gctgatcaac ggcgaacgca tctatccccc
      961 gctcatcggc ggccgcgccg ggcttctcgc cgcggccgcg ccgaacctac aggcgccgcc
     1021 tgcttacggg cgatgagcgc gatatgacag cgtgacaccg ttcgcggttg tgctcgatca
     1081 tgtcgggttg atctcgtcca agctctttcc cgtggttcgg ggcccaatcg gaataagcac
     1141 ggcgaccgcc gcgaccagcg cgacgaggac gacgccgaac atcgcggtgg cgccgtagtg
     1201 attgagcacc gggatgagga tgaacggaag cgccgcgctc gaaagccgag acaccgagta
     1261 ggtccatccc accgcggtcg cccgcacatc actggggaag atctccgcct ggtacacgtg
     1321 gtagatgttc gagaagacat tgctcgccgc agtagtcagc aatccgaaca cgacgatcat
     1381 caccggcacg ctcaccaccg cgaagagcgg gccacacacc gccatgacag cgatcgtcgc
     1441 catgatcagg tacttgcgtt cgaaccgctt cagaagcgga atcgagatga gtgacccgag
     1501 cggatagccg atgaacgaca ccgcggtgaa gagcagcgat gacgtcacgt cgtagccacg
     1561 actgaccagc accagggccg ccagcgtgcc gaatccgtag tagccgaaag gctggaacag
     1621 atggaacacc acaagcatga acagacgctg ccggtaaggc gagagcctca gccgggcaat
     1681 cgcattcacg gtgtgagtcg gctctgaagc atgctccggc acgtcctcga catcgaggtc
     1741 gacgtcggcg acctccgctg tgttcgagtc ggttccggat tcgaacgccc tcatcgcggc
     1801 ctccgcttcc tcggtacgac cgacacccgc cagccacctc ggcgactcgg gcagtgcccg
     1861 gcgcaacacg atgacgaaca gcgcaccgat cgcgccgatc accagaagca ctcgccaact
     1921 gtcgaatccg agcaccttgt ggccgttgag ggacagcgtg agaaatccca gtgcgggcac
     1981 ggcgaggaac gagcaggtgt aggcccaggc ggccaagcgg ccgcggtgat ccttcggcag
     2041 cacgtccgac aggtatgagt cggcaaccgg atattccgcg ccgacaccga tgcccgccat
     2101 gaagcgggct ccgacgagca tcaacggatt cggcgcgatg gcgccgatga tgctccacac
     2161 cgagaaccac accaggttga gcaggaaggc ccgcctgcgc ccgatgcgat cggcgatccg
     2221 gccgaatgcc gctgcaccga tgaacattcc gagaaacgac gatgccatca gcaactgcag
     2281 tgccgtgccg ccgacgccgt actcggtctt cagtgcggtg gcgatcgtgc cggacaggaa
     2341 gatctcgtaa acctcgaaga acaggcccag tccgatcgcg acgacgatct tgcggtggat
     2401 gcgcccgacc ggaaggcggt caaggcgtcg accgatgtcg tgcagcccga ccttgcggcc
     2461 gggctgcacg agggtggcgg tcacttcgag tctccgttgt catcggactg gctgtcgctg
     2521 ctgtggtcgt tggacgagtt actgctgtcg tgatcacccg acgagtggct gttgtcgctg
     2581 tcgtggtcgc ggttccagaa gttccagcgg ttgtcgtgat caccgtgccg gcgatagctg
     2641 ttgtcgtccc ggtggttcca ccagtagttg ccgctgttgt cccactggga gtcgtgatcc
     2701 gagtgccccg actgatcgct tggctgctgc gaaggtgcgg gcggaggcgg cgggctctgc
     2761 ggcggcgcct ggtggctggc cggcttggag tatgcgccgc tcgacatcgt cgagtgcgac
     2821 gacgaccggt ggcggggcgc cgccggctgg acggcgggca tgggtgcggg tgccggtgcc
     2881 ggcgccggcg ccgcggcggg cgcggtggcg ttgtgggcgg ctgtgttcac cggcgtgctg
     2941 ctcgaactgt gcagcgcacc gaacaggccg gccgccgcgg cggcgacgag gcccgcgccg
     3001 gcgaaggcca gcacacgggt gcgggcgtac cagggttgct gcgccggata ggccgccgac
     3061 accgagttga attccggctg ctcggccgac catgccagcg caggcatggg tgcctggccg
     3121 tacgccgggc cgtacgcgta tgcatgaggc ggcgcgacgg gggccgtcgg accggccatc
     3181 gtcggcggga agtaggccgc catggtcgga acgtccgaag acgtctgcca ggaagcggcc
     3241 tgcgtcgcgg tccagttcct gttttggttc gaagggaagg tggtcatttc gtgctccttg
     3301 tttcagtggt cccggtgctt gtgccgcacc gctgattacg aagttaggga gctcgcgacc
     3361 caaaggcccg cttgtgcgca caaggggtcc taacaggcga tttagctctt ttgcgcattc
     3421 tgctcaagca gcggagagag tcggcgcgaa aagtagggca gcgaaggact caggcggttt
     3481 cggacacctt caggtcacgc cctgacaacc gcatgtaggt tgcgacgccg agaccgatga
     3541 tgagccagat gattccgacg accttggcgt tggtgtctgc atcccacagc gcgaccatca
     3601 ggatcgcggc gccgagcagt ggggagatca catgggtgaa ccaccggcgg cttccgttgc
     3661 gcttgaagaa gtgcgtgatc acgctgaagt gcatcaggat gtacgacagc agtgcaccga
     3721 atgtcaccag cgtggtgagc aggctcgcct ggcccactcc gaagacgccg atcgccagcg
     3781 caagcgcgca gatgatcacc aacgcgttca cgggcacttt gcgcttgccg ctcacccggg
     3841 cgaagagctt cggcagatgg ccgtcgcgcg ccatgctgta caccagccgg gacgcggttg
     3901 cattggcggc gacgatgttg cccagcgcgg cgaacagcgc cgagttcaag acgatgaaca
     3961 tcttgaactt gctatccatg atgttgccca ccaggtcgta gaaggcgttg ttggcggccg
     4021 tgccgtcggc gtaatgggtt ccgctcggca ccaggatgca ggcgaagtag acctgcacga
     4081 caaacaacac cgtcgagagg tacatgacca gcatcgtggc cttggacacc gccttaccac
     4141 cgcccttggc ctcttcgttg agcgtcgaga cggcgtcgaa cccgaggaag ctgaacgccg
     4201 ccaccgggat ggcgcctgcc accaccgacc atgacgctcc gccgtgtgga tggaacgggt
     4261 ccagcgtcag gtgggcctgg ccctgcaaca ccagagacgc tgcccagcag aggaacacag
     4321 ccaggatggc gagctggatg tacagcgagt acttgttcat cttggccgcg acgttcatgc
     4381 cgagcaggtt cgtcaccgcg gtgaccgcga cgaagaagac cacccacgtc cacgccggta
     4441 tgaacggcac gatggtggtc atcgcgctcg ccgagaacac gcacaacagc gcgggcagga
     4501 gcagatagtc gagcaggatc gcccagccgg agacgaatcc gaggaagcgg ttcgtcccga
     4561 ggctgacata gctgtaggtc gatcccgaga ccggaaaaac ccgggccatc tccttgtagc
     4621 tgatcgcgct gaaaagcatg gcaaccgcgg cgatcacata gacggcggct accgcccccg
     4681 ccgacaggtt gtagagaatg ccgaacacgc cgatcggagc cagcggcacc atgtagatca
     4741 atccgtggat caccaagtcc cgcacggaca gagtccgtgc gagttcgggc tcgtagccga
     4801 gttcgcgcaa ccgcgcgcag tcatcgtcga gttgcatcag cattgcggtc tccttgtcgg
     4861 tgcgacaccg acgggatacg gcggcgcgcg gttgcgactg aagctacgga ggcgatgacg
     4921 tggtgactcg tttgtgcgca caagccgcac aacaggcgat ctcgatgttt tgcgcattgt
     4981 gctcagcaag acacgtacgc tcttcgcatg gtgcggatgc ggcccgtgga cgtgcggctc
     5041 gcgacgcagg tgctggtgct gcaggtcgcc gtggtggccc tcacgctggg cctcgcgggc
     5101 ggaatgctcg ccttcttcag ccacgaccgg cttgcgtccc agtacgagga tcaatcgctg
     5161 gatgtggccc gtgcggtcgc cttcgcgccc gcggtacgcg ccgacgtcac ccgttatgac
     5221 gacgcccctt tgacgccgag ccctgcactc accgcggagt tggccggcgg tccgttgcag
     5281 acgctggcca ccgagattca gaagcacgca aacctgttgt tcgtggtgat caccaacgac
     5341 acgggcatca ggctgtccca ccccgaccgc aacgaactcg gcaagcacgt cagcaccgat
     5401 ccctccgaag ccttggcggg ccacgaggtg gtggaccggg agtccggcac actgggcagc
     5461 tccgtccgcg cgaaagttcc catctacgca ccgaattcga ctcgagtagt aggtgaggtc
     5521 agcgtcggta tctctaccga gacggttcac catcagctgt ggtcggatgt gcgccgggcg
     5581 gcggttctgg tcggcgtggc gttgctcatc ggtgtcgtcg gctcggtgct tctggcccgg
     5641 cgatggcgcg gcctgacgtt ggggctgcaa ccttcggaga tggccgagtt gatccgcggc
     5701 caggcggccg tcctgcacgg catcgacgag ggcgtgctgg ccgtcgacac cgaatggaag
     5761 accactttcg tcaacgacct ggcgtgccgc atgctcggga tcgacgatga gaccggcagg
     5821 ccggtggaag agatcggcct gacgccgcgc gtgctcgaag tgttcaagtc ggcggagtcc
     5881 acccccacgc tggccaccgt cggtgatcgc atcgtgctgg tgtcggcacg acgcgtggcg
     5941 cgcgagggcc gcgagttggg aaccgttctc gtggtgcgcg atcgcaccga cgtcgaatcg
     6001 ctgacccgcc agctcgatgc ggtgcagttg atgagcaccg tcctgcgggc ccagcgccac
     6061 gagttcgcca accggttgca tctgctcaac ggtcttctcc acagcggcca cgttgaggag
     6121 gccggccagt atgtggagga gctgttgggc tccggcccgc tgggatcggc gataccgggc
     6181 atcgacgcca ttcgcgacgc gtttcttcag gcgttcctgg cagccaagtc ggcgtccgcc
     6241 cgggaatcgg gtgtgacgct gaccatcggt gagaacacct gggtgccggg cagactcgcc
     6301 ctacccgtgg acgtgacgac cgtgctgggc aacctgctcg acaacgcgat ctacgccgcg
     6361 cgcaccgggg acagtgacgc caaggtggtc gaagccgagc tgctacagga cggttcaacc
     6421 ctgcacatca cggtggtcga cacaggcgac ggcgtgccgc ccaacttcgt cgatcacctc
     6481 ttcaccgagg gcaagtcgac gaagccggac tccggaatac ccggtgggcg tggcatcggt
     6541 atggcgttgt ctcgtcaaat catccgcgca ctcgatggcg aaatccgctt gtcgagcccc
     6601 ggcaatccgg gagcgcatct gtgcggagcg gaattcatcg cccgtttgcc gggtgtgatg
     6661 gtagaggagg aagcccaatg ggccgctcag aattgactgt gcttgtggtc gacgacgact
     6721 tccgggtcgc caacatgcac gcggggatcg tcaacgccct gccgggcttc acggtgacga
     6781 cgacggcgac gacactttcc gcggcacgca aggccgacct ggtcgatctc gcgctggtgg
     6841 acgtgtatct gcccgacggg tcgggcgtcg atttcgtccg cgagttgcgc tgcgacagca
     6901 tggttttgag tgcggccacc gacgctccga cgatccgagc cgcgattgcc gccggagcgt
     6961 tgagctatat cgtcaaacca ttcgcacccg cggacctcgc ggccaggctg ggcggctacg
     7021 cgcgctaccg taagctgctg gccggaacca atctcggtcg caaggaagtg gacgccgcac
     7081 tcgatgcgtt gcgtccgaag atcgcgcctg tccagtcccc gaccgcggtg gcgtcgccga
     7141 cgaaggacct tgtgctgcag gcgcttcgtg catctggcca accgatgtcg tcggccgagg
     7201 tgtccacgga gatcggcgtt tcgcgggcca ccgcacagcg atatctctcc acg
//
LOCUS       DASYLX010000547         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_685601,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000547 DASYLX010000000
VERSION     DASYLX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685601"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGP27_13240"
     CDS             <1..228
                     /locus_tag="VGP27_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852296.1"
                     /translation="FLTMDRWLMWRWPTTGRFLEYMKITYGWSLMLVVVFCVLYFRYL
                     DAKRENRLESGIDPPWLTDTRPNESRPVRSG"
     gene            306..722
                     /gene="msrB"
                     /locus_tag="VGP27_13245"
     CDS             306..722
                     /gene="msrB"
                     /locus_tag="VGP27_13245"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV7852297.1"
                     /translation="MTSPQRRVELTDDEWRKKLNPAEYAVLREAGTERPFTGEYTDTK
                     AEGVYECRACGAELFRSTEKFESHCGWPSFYDPANTDAVILREDDSLGMKRVEVLCAN
                     CHSHLGHVFEGEGYPTPTDQRYCINSISLRLAPAGP"
     gene            complement(682..>2691)
                     /locus_tag="VGP27_13250"
     CDS             complement(682..>2691)
                     /locus_tag="VGP27_13250"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEV7852298.1"
                     /translation="GHSRIRYVLDGLIVAGALFEISWVLVLRTVYDAGNTSRFALGLS
                     LAYPIADIAILTVAAIVLTRARTGRRSTLALLTGGVVLMALSDSAFVYLTAQGTYSSG
                     HIIDIGWAAAFLTFGMAALNSRVSPETEVVVQQVPSRMSMWLLYVPLAIAALVCTPAY
                     LPTPGLGPIFVSSTLLMTAVLARQYTVVRQNQRLLKMVADQALRDTLTGLANRDLFND
                     RLMHAVQLHENDSQSVAVLSMDLDDFKLVNDSLGHPAGDALLIQAAERLLGCVRTSDT
                     VARVGGDEFAVLMEGLPELSRLVAHRVVAAFEEPFLIDGQQLLMRPSVGLAVASSANP
                     DLSADLLLKQADVAMYSAKRSKSGGLHTFTADMALSDPNEIELPTEKSEHTERGAAVR
                     LLGELRHAIDHGDLTVFYQPKFDLRTGDVVGVEALVRWPHPKRGLLGPAHFLPLVRKR
                     GLMRAVTDIVLTQALDDAAEWQGMGIGVPIAVNVFAPSLGDLDLPTHIASALAMRRLS
                     PEGLTIEITEDFLLDNIDRTRSVLERLRQRGIRIAIDDFGSGYSALWYLRELAIDEVK
                     LDRHFIAPIRVDARAAAVVRGVVDLAHVLGVTTVAEGVENAETAERLREFGCDVAQGY
                     YYSPPVSAAAMMNMLAAGQPKRGTQVEFEKLGATDPQVPVVAKSS"
BASE COUNT          503 a          894 c          852 g          442 t
ORIGIN      
        1 ttcttgacaa tggaccgctg gctgatgtgg cgctggccga ccaccgggcg ctttctggag
       61 tacatgaaga tcacctacgg ctggtcactg atgctcgtcg tggtgttctg cgtgctgtat
      121 ttccgctacc tggacgccaa acgggagaac aggctggagt cgggtatcga cccgccatgg
      181 ctgacggaca ccagacccaa cgaatccagg cctgtccgct cgggatgaca ccggtagccg
      241 ccgccctcgt tcgctgcgac tcgtcggcga aacgggacgc ggcgtaactg cgctagcgtg
      301 gacatatgac ctctccgcag cgcagggtgg aactcaccga tgacgagtgg cgcaagaagc
      361 tcaaccccgc cgaatatgcg gtgctgcgcg aagcaggcac cgagcggccc ttcaccggtg
      421 agtacaccga caccaaggcc gaaggcgttt acgaatgccg ggcctgtggc gccgagttgt
      481 tccgcagcac cgagaagttc gaatcccatt gcggctggcc atctttctac gacccggcca
      541 ataccgacgc ggtcatcctg cgggaggacg attcattggg catgaagcgc gtcgaggtgc
      601 tgtgcgccaa ctgccacagc catctcggcc acgtgttcga gggcgagggc tacccgacgc
      661 ccaccgatca gcgctactgc atcaactcga tttcgctacg actggcacct gcgggtccgt
      721 agcgcccagc ttctcgaact cgacctgcgt accgcgtttc ggctggcctg ccgccagcat
      781 gttcatcatc gccgcggcac tcaccggcgg gctgtagtag tacccctgag ccacatcgca
      841 gccgaactcc cgaagtcgct ccgcggtctc tgcgttctcc acaccctcgg cgaccgtcgt
      901 cactccgagg acatgggcga ggtcgacgac gccacgcacc acggcggcgg cgcgggcgtc
      961 gacgcggatc ggcgcgatga agtgcctgtc gagtttgacc tcgtcgatgg caagttcccg
     1021 cagataccac agcgccgagt agccgctgcc gaaatcgtcg atggcgatcc gaattcctct
     1081 ctgccgcaac ctttccaaga ctgaccgcgt gcggtcgatg ttgtcgagga ggaagtcttc
     1141 ggtgatctcg atcgtcagac cttcggggct cagcctgcgc atggcgagag ctgaggcgat
     1201 atgggtgggc agatccaggt cccccagcga cggtgcgaac acgttcaccg cgatcggaac
     1261 gccgatgccc atgccctgcc attccgcggc atcgtcgagg gcctgcgtca gcacgatgtc
     1321 ggtcaccgcc cgcatcaggc cgcgcttgcg aaccagcggt aggaagtggg ccgggccgag
     1381 cagtccgcgc ttcggatggg gccaacggac cagggcttcg accccgacga cgtctccggt
     1441 gcgcaggtcg aacttcggtt ggtagaagac ggtcaggtcg ccgtggtcga tggcgtgccg
     1501 aagctccccg aggagtcgca ccgccgcacc gcgctccgtg tgctcgctct tctcggtcgg
     1561 caactcgatc tcgttcgggt cggacagcgc catgtctgcc gtgaaagtat gcaggccacc
     1621 ggatttcgac cgtttggccg agtacatcgc cacgtcggcc tgtttgagca gtaaatccgc
     1681 ggacagatcc ggattggccg acgacgcaac ggcgagcccg acgctgggcc gcatcaacag
     1741 ctgttgcccg tcgatcagga aaggctcctc gaacgcggcg accacgcgat gcgcaaccag
     1801 gcgcgacagc tccggcaagc cctccatcaa cacggcgaac tcgtcgccac ccacccgcgc
     1861 caccgtgtcg gaggtgcgca cgcagccgag cagcctctcc gcggcctgga tcagcagggc
     1921 atcgcccgcg ggatggccaa ggctgtcgtt gaccaacttg aaatcgtcga gatccatcga
     1981 cagcaccgcc acggactggc tgtcgttctc gtgcagctgc accgcatgca tcagccggtc
     2041 gttgaacagg tcgcggttgg ccaggcccgt gagcgtgtca cgcaaagcct ggtcggccac
     2101 catcttcagg agccgctgat tctgccgcac gaccgtgtac tgcctcgcca gcaccgcggt
     2161 catcagcagt gtcgacgata cgaagatggg tcccagaccg ggagtcggta gatacgcggg
     2221 cgtgcacacc agggcggcga tggcgagcgg cacgtagagc agccacatcg acattcggga
     2281 cggtacctgc tgcaccacca cctcggtctc cggagagaca cgactgttca gagcggccat
     2341 gccgaaggtc agaaaagccg ccgcccaccc gatatcgatg atgtggccgc tggaatacgt
     2401 cccctgggcg gtgaggtaca caaacgcact gtcggacaac gccatcagca caacgccgcc
     2461 ggtcagcagc gccagcgtcg agcgcctgcc ggtgcgagcc cgggtcagca cgatggccgc
     2521 gacggtgagg atcgcgatat cggcgatcgg gtaggccagc gagagaccca gcgcgaatcg
     2581 gctcgtgttg cctgcgtcgt aaacggtcct cagcaccagc acccaggaga tctcgaacag
     2641 cgcgccggcg acgatcagcc cgtcgagcac atagcggatc cgggaatggc c
//
LOCUS       DASYLX010000548         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2111730,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000548 DASYLX010000000
VERSION     DASYLX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111730"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3542
                     /locus_tag="VGP27_13255"
     CDS             <1..3542
                     /locus_tag="VGP27_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEV7852299.1"
                     /translation="PATAWQLDVFGHDPQFPGMAADAGLTSSSWARGPHHQWGPMQNQ
                     GDPERMQFSSEFEWISPSGRGLLTHYMPAHYSAGWWMDSSATLAEAETATYELFSTLK
                     KVALTRNVLLPVGTDYTPPNKWVTEIHRDWNERYTWPRFVCGLPREFFASVRAELNAR
                     GIEASPQTRDMNPIYTGKDVSFIDTKQANRDAENAVLDGERFALFAGLLGGATYPQAA
                     LEKAWVQLAYGAHHDAITGSESDQVYLDLLTGWRDAWELGRATRDNALAVISTAVEGS
                     AVVWNTLAHSRTDVVTARLDEPFGDGVQVTDADGNELPALVEHGGRSVTWLARDIPSL
                     GWRAYHLTAGPVDAGWEGLSGNEIGNERYRLRVDPARGGAVSSLTDDGRELLAAGKVG
                     NELAVYDEYPAHPEAGEGPWHLLPKGPVVLSSSTPAESVHRYRCALGERLVVRGRIGS
                     VLRYTQTLTLWRGVPRLDCSTTIDEFTGEDKLLRLRWPCPIPGAMPVSEVADAVIGRG
                     FALLHEPGSDESVDTAKHPYTLDNPAYGWFGLSAAVRVRVGHSVRAVSVAEVVSPAEG
                     TSAPLARDLMVALVRAGVTATCSSADTPRYGDLDVDSNLPDARIALGGPDQNSFTAAV
                     LAEADPAYSDELKRQLHATGSARVWVPAAAPLAATWRPGANLRGVRALPVLVVAGDDL
                     DEAIASVVEDLADAEIPVDQQVPSDIEAFERRTVAVLNRGVPSFAVDADGTLHTSLMR
                     SCTGWPSGVWMDPQPRRTAPDGSSFQLQHWTHTFDYALVSGDGDWRAAAVPNRSAEFS
                     HPMLTVVGKGSTTGGLPSWGSLLEIEPAGTVQLAALKAAGNPLASGRSQPVDPAEAVA
                     MRLVETVGATTDVAITSGLHSVSSASRVNLLEEPRLQKHRAFEGLTLHGYEIATVLTR
                     LNMPRLVEADHTQLAPEAEAAQPLYARYWLHNRGPAPLGGLPAVAHLHPQATVAEPND
                     TVVLRLTAASDCTDSVLHGTVRLACPDGWSASPATLPFVLPSGEYLEADVELTMPQDT
                     APGLYPIRAQLVVTGDDAASMPASWRQMVEDVATVSVGTPREDNDIVYLIGEPADVGV
                     VAGETARLAVTVGTSAGASLSLEAHLISPWGTWEWIGPAAQGAVLTPGETVELGFDVA
                     PPPWVQPGEWWALVRVGCAGRLLYSPAVRLTVR"
     gene            3539..4159
                     /locus_tag="VGP27_13260"
     CDS             3539..4159
                     /locus_tag="VGP27_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-ACP transacylase"
                     /protein_id="HEV7852300.1"
                     /translation="MSHPHDAATVGGRAISVADVDAREERVRAAVPAAALPRVGTSEG
                     RQLRRWLTQLLVTERLIGTEAAAMGLSADGAPAEAQLLPDNSARFEIGSVAAAALADP
                     LARAMFVRVTAGVDVTDADVADYHARNPLRFAMPADAGNGWRTTTVDVPPLETVRPLI
                     VGQLRGAARRRAFRLWLDARRAELVRLAAGYEHPGDPGQPDNTHRH"
     gene            4159..>4439
                     /locus_tag="VGP27_13265"
     CDS             4159..>4439
                     /locus_tag="VGP27_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7852301.1"
                     /translation="MPATALAIDIGGTKIAVGLVADDGELGHHAQLPTPDGDAEAIWA
                     VVDTLVTEAVAAAGGYVRGVGIASAGPIDLPSGTVSPINIPQWHRFPIV"
BASE COUNT          660 a         1516 c         1598 g          665 t
ORIGIN      
        1 agccggcgac ggcgtggcag ctcgacgtgt tcggccacga tccccagttc cccggcatgg
       61 ccgccgatgc cggcttgacg tccagctcgt gggcgcgcgg gccgcaccac caatggggcc
      121 cgatgcaaaa ccagggtgat cccgagcgca tgcagttctc cagcgagttc gagtggatct
      181 ccccgtcggg ccgcgggctg ctgacccatt acatgcccgc gcactattcg gccggctggt
      241 ggatggattc gtcggcgacc ctcgcagagg cggaaaccgc gacctacgaa ctgttctcga
      301 cgctgaagaa ggttgcgttg acgcgcaacg tgctgctgcc ggtcggcacc gactacacgc
      361 cgccgaacaa gtgggtcacc gagatccacc gcgactggaa cgagcgctac acctggccgc
      421 ggttcgtctg cgggctgccg cgggagttct tcgcgtcggt gcgggctgag ctgaacgcga
      481 ggggaatcga ggcgtcaccg cagactcgcg acatgaaccc gatctacacc ggcaaggacg
      541 tctccttcat cgacaccaag caggccaacc gcgacgccga gaacgcagtg ctcgacggag
      601 agcggttcgc cctgttcgcc gggctgctcg gcggcgccac ctatccgcag gccgcgctgg
      661 agaaggcgtg ggtgcagctg gcctacggcg cacaccacga cgccatcacc ggttcggagt
      721 ccgaccaggt gtacctggac ctgctgacgg gctggcgcga tgcatgggag ctggggcgcg
      781 cgactcgcga taatgcgctg gccgtgatct ccaccgctgt cgaaggatcg gcggtggtgt
      841 ggaatacgct ggcgcacagc agaaccgatg tggtgacggc gcggctggac gagccgttcg
      901 gcgacggtgt gcaagtgacc gacgccgacg gcaacgagtt gcctgcgctg gtcgagcacg
      961 gcggccgttc ggtcacctgg ctggcgcgcg acatcccgtc gctcggctgg cgcgcctacc
     1021 atctgacggc aggtcctgtg gatgcgggct gggaagggtt gagcggcaac gaaatcggca
     1081 acgagcgtta tcgtctgcgg gtcgacccgg cgcgtggcgg agcggtgtcg tcgctgaccg
     1141 acgacggccg tgaactgctc gccgccggta aggtcggcaa cgagctggcg gtctatgacg
     1201 agtatcccgc gcatccggag gcgggcgagg gaccctggca tctgctgccc aaggggccgg
     1261 tggtgctctc gtcgagcacg cccgcagaat ccgtgcaccg gtaccggtgc gcgctcggcg
     1321 agcgactggt cgtgcgcggg cggatcggtt cggtgctgcg ctacacccag acgttgacgc
     1381 tgtggcgcgg cgtcccgcgc ctggactgca gcaccacgat cgacgagttc accggcgagg
     1441 acaaactgct gcgcctgcgc tggccgtgcc ccatccccgg ggcgatgccg gtgagcgagg
     1501 tcgccgacgc ggtgatcggg cgcggcttcg ctctgctgca cgagccgggt tccgacgagt
     1561 ccgtcgacac cgcgaaacac ccttacacgc tggataatcc ggcctacggg tggttcgggc
     1621 tgtcagcggc ggtgcgggtg cgcgtcggcc actccgtacg ggccgtttcg gtcgccgagg
     1681 tggtgtcgcc ggccgaggga acatcggcgc ccttggcccg cgacctgatg gtggcgttgg
     1741 tccgcgccgg cgtcacagcc acctgcagca gtgccgacac gccacgctac ggagacctcg
     1801 acgtcgactc gaacctcccg gatgcccgga tcgcgctcgg cgggccggat cagaactcct
     1861 tcacggcggc cgtattggcc gaggccgatc ccgcctacag cgacgagctc aagcgtcaac
     1921 tgcacgcgac cggctcggcc agggtgtggg tgcccgccgc cgcaccactg gcggcgacgt
     1981 ggcggcccgg tgcgaacctg cgcggtgtgc gggcgttgcc ggtgctcgtc gtcgcaggcg
     2041 acgacctcga cgaggccatc gcctcggtcg tcgaagacct cgccgacgcc gagattccgg
     2101 tcgatcaaca ggtgccgtcg gacatcgagg cattcgagcg gcgaacggta gcggtgctga
     2161 accgcggggt gcccagcttc gccgtcgatg ccgacggcac cctgcacacg tcgttgatgc
     2221 gctcgtgcac cggctggccg tccggggtgt ggatggaccc gcagccgcgt cgcaccgcgc
     2281 ctgacggctc gagcttccaa ctgcagcact ggacccacac cttcgactat gcgttggtat
     2341 ccggcgacgg tgactggcgc gcggccgccg tcccgaaccg cagtgcggag ttctcccatc
     2401 cgatgttgac cgtcgtcgga aagggcagca ccacaggcgg tttgcccagc tggggctcgc
     2461 tgctggagat cgagcccgcg ggcactgttc aactggccgc gttgaaggcg gcgggcaacc
     2521 cgctggcatc cggtcgctcc cagcccgtcg accccgcgga agcggtggcg atgcggctcg
     2581 tcgaaacggt gggggcgaca accgatgtcg cgatcacgtc cggcctgcac agtgtgtcgt
     2641 cggcgtcgcg cgtgaacctg ctcgaggagc cgcgcctgca aaagcaccgc gccttcgaag
     2701 gcttgaccct gcacggatac gagatcgcca ccgtgctgac ccgcctcaac atgccgcgac
     2761 tcgttgaagc cgaccacacg cagctcgcgc cggaagcgga ggcggcgcag cccttgtacg
     2821 cccggtactg gctgcacaac cgcggcccgg cgccgctcgg cgggctgccc gcggtcgcgc
     2881 acctgcaccc gcaggcgacc gtcgccgagc ccaatgacac tgtcgtgctc cggctcaccg
     2941 cggccagcga ctgcaccgac tcggtgttgc acggcacggt gcgcctggca tgtcccgacg
     3001 gatggtccgc gagcccggcg acgctgccgt tcgtgctgcc cagcggtgaa tacctggagg
     3061 ccgacgtcga gctgacgatg ccgcaggaca ctgcgccggg gctctatccg atccgcgctc
     3121 aactcgtcgt caccggcgac gacgcggcgt cgatgcccgc gtcgtggcgg cagatggtcg
     3181 aggatgtcgc gacggtgtcg gtcggcaccc cgcgcgagga caacgacatc gtctacctca
     3241 tcggcgagcc ggcggatgtc ggggtggtcg cgggagagac cgcgcggctc gcggtgaccg
     3301 tcggcacctc ggcgggtgcg agcctgtcgc tggaggcgca cctgatcagc ccctggggca
     3361 catgggagtg gatcggtccg gcggcgcagg gcgcggtgct cacacccggc gagacggtgg
     3421 aactgggctt cgatgtcgcc ccgccgccat gggtgcagcc gggcgagtgg tgggcgctgg
     3481 tgcgggtggg ttgcgccgga cggctgctct actcgcctgc cgtccggctg acggtccgat
     3541 gagccacccg catgacgccg caaccgttgg cggccgggct atatcggtcg ccgacgtcga
     3601 cgcgcgggag gagcgagtgc gggcggcggt gcccgccgct gcgctgcctc gcgtgggtac
     3661 cagcgaaggc aggcagttgc gccgctggct gacgcagctg ctggtgaccg agcggctgat
     3721 cggcaccgag gccgccgcca tgggactgag cgcggacggt gcgcccgcgg aagctcaact
     3781 gctgccggat aattcggccc ggttcgagat aggcagcgtc gccgcggcgg cactggccga
     3841 tccgctcgcc agagcgatgt tcgtccgcgt cacagccggc gtcgacgtca ccgacgccga
     3901 tgtggccgat taccacgccc gcaacccgct gcggttcgcc atgccggcgg acgccggaaa
     3961 cggttggcgc acaacgacag tcgacgttcc gccgttggag accgtccggc cattgatcgt
     4021 cggacaactt cgcggtgccg cacgtcggcg tgccttccgg ctgtggctgg acgcgcgacg
     4081 ggccgagctg gtgcggctgg ccgccggcta cgaacatccc ggcgatcccg gccagcccga
     4141 caacacgcac aggcactgat gcccgccacg gcgctggcaa tcgacatcgg cggcacgaag
     4201 atcgccgtcg ggttggtcgc tgacgacggc gaactgggcc accacgccca gttgccgacg
     4261 ccggacggcg acgccgaggc gatctgggcg gtggtagaca cgttggtcac ggaggcggtt
     4321 gccgccgcgg ggggttacgt ccgcggtgtc ggcatcgcgt cggcgggtcc catcgacctg
     4381 ccgtcgggca ccgtcagccc gatcaacatc ccgcagtggc accgctttcc gatcgtcga
//
LOCUS       DASYLX010000549         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_904575,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000549 DASYLX010000000
VERSION     DASYLX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904575"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VGP27_13270"
     CDS             <1..1255
                     /locus_tag="VGP27_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013989027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852302.1"
                     /translation="CGAGYLLAGEWLIRRLFAVYGPIVSIPLPGIPVVLIKDPELVKQ
                     VFTEKPDILLGGKGVRPSALIYGTGSMFVQEEPEHLRRRKLMTPPLHGRALENYRPII
                     EDSTLHALASWPRDEPFTMLRAARDLALDIIVRVMFGVEDRNERNRLGRPFEALLDLG
                     SSEQLILRVALHSVGGLKRWPRLDRANQGIDDLLTPMIAARRRDPDLRERTDIMSLLL
                     SIRSEDGEYLTDKEIRDDLVTLVLAGHETTATTLAWMIDLLLHHPHELAAVQEEAEAA
                     GPTAYVQAVINETLRFRPPSPFTGRYTAGDYQLGGYTVPARTRIVPHIGEVNLDAGTY
                     DHPHEFRPERFLKTSPPTYAWIPFGGGIKRCLGASFSTLELTVVLQTLLRSGRFEAVH
                     ETQDRQVRRGIVLLPHRGVPVRYRR"
     gene            1302..2561
                     /locus_tag="VGP27_13275"
     CDS             1302..2561
                     /locus_tag="VGP27_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV7852303.1"
                     /translation="MEIDPAATAWLLACTALVLLMTPGLAIFYGGMVRSTGVLNIIMM
                     CFISIPVVTIVWLLAGYSLAFGPDAGGGLIGNLDHFGLLGIDATTVRSGIPELLFVTF
                     QLGFAIVTAALICGAIADRARFSAWMIYIAVWTVAIYAPVAHWVWGPNGWLASLGTVD
                     YAGGLVVEIVSGASALALALVLGPRIGFKSEAMRPHNLPFVLLGVGLLWFGWFGFNAG
                     SALAADGKASVVFLNTLIAGCLGMMGWIFVERVRDGRPTTFGAASGVIAGLVAITPSC
                     GFVAPLGAFAIGLAAGVVCSYAVGWKFKAGYDDSLDVVGVHFVGGVVGTFLIGLLAAE
                     VVSGGVEGLFYGGGLALLGKQTLAIPVVALYAFIVSFIIGKVIEATFGFRIEREDEIS
                     GIDLAQHAETAYTEGVHGAPQKRPGIG"
     gene            complement(2574..>2858)
                     /locus_tag="VGP27_13280"
     CDS             complement(2574..>2858)
                     /locus_tag="VGP27_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV7852304.1"
                     /translation="AQSLGLVAGGRARITTAAGSAEASVEISEAMLPGHASLPNGLGL
                     DFVDDEGQTHVPGVAPNALTSTEWRDAYAGTPWHKHVPARIEAVEAAAAV"
BASE COUNT          451 a          928 c          919 g          560 t
ORIGIN      
        1 ttgcggtgcc ggatatctgc tcgccggaga gtggttgata cggcgcctgt tcgccgtgta
       61 cgggcccatc gtgtcgattc cgctaccggg catcccggtg gtcctcatca aggaccccga
      121 gttggtcaag caggtgttca ccgagaaacc cgacatcctg ctcggcggca agggcgttcg
      181 gccttcggcg ctcatctatg gaacgggctc catgttcgtt caagaagaac ccgaacacct
      241 tcggcggcgg aaactgatga cgccgccgct gcacggcagg gctctggaaa attaccggcc
      301 gatcatcgag gacagcaccc tgcatgcgct cgcctcctgg ccgcgagacg agccgttcac
      361 gatgctccgc gcggcgcgcg acctcgctct cgacataatc gtccgggtca tgttcggcgt
      421 cgaagatcgc aacgagcgca accggctcgg ccgtcctttc gaggcgctgt tggatctcgg
      481 gtcctctgag caactgatcc tgcgtgtcgc cctgcactcg gtaggcggct tgaagcggtg
      541 gccccggctc gaccgcgcga accagggtat cgacgatctg ctgacaccga tgatcgccgc
      601 cagacgccgg gaccccgatc ttcgcgagcg caccgacatc atgtcgttgt tgctcagcat
      661 tcgcagcgag gacggcgagt acctgaccga caaggagata cgcgacgacc tggtgacgct
      721 ggtgctggcc ggccacgaga cgacggccac gacactggcg tggatgatcg acttgctgct
      781 gcaccaccca cacgagctgg ccgcggtgca ggaggaggcc gaggcggccg gaccgacggc
      841 gtatgtgcag gcggtgatca acgagacgct tcgtttccgg cccccctccc ccttcaccgg
      901 gcgctacacc gccggtgact atcagctcgg cgggtacacg gtgccggccc gaacgcgcat
      961 cgtcccgcac atcggcgagg tgaacctcga tgctgggacg tacgaccacc cgcacgagtt
     1021 ccgacccgag cgattcctga agacgagccc gccgacctac gcatggatcc cgttcggcgg
     1081 cggaatcaag cgttgcctcg gcgccagctt cagcactctc gagctcacgg tcgtgctcca
     1141 gacgctactg cgctccggcc gattcgaagc cgtgcacgag acgcaggatc gacaggttcg
     1201 tcgcggaatc gtcttgctgc cgcatcgagg ggttcctgtt cgctatcgcc gctagcacgc
     1261 cataatctgg cgagcgtttg cgaaagacaa aggagtcacc gatggagatc gaccctgccg
     1321 caaccgcctg gctgcttgcc tgcacagcac tggtcctgtt gatgacgccg ggtctggcca
     1381 ttttctacgg cggcatggtg cggtcgacgg gcgtactcaa catcatcatg atgtgcttca
     1441 tctccattcc ggtcgtcacg atcgtgtggt tgctcgccgg ctattcgttg gccttcggcc
     1501 ctgacgccgg cggcggtctc atcggcaacc tcgaccactt cgggctactc ggcatcgacg
     1561 caaccacggt tcggtccggc attccggaat tgctcttcgt gacctttcag ctcggctttg
     1621 cgatcgtcac ggccgcactc atctgtggcg cgatcgccga ccgcgcccgg ttctcggcat
     1681 ggatgatcta catcgccgtc tggaccgtgg ccatctatgc acccgtcgcg cactgggtgt
     1741 ggggacccaa cggctggctc gcgtcgctgg gcaccgtcga ctacgcgggc ggtctcgtcg
     1801 tcgagatcgt gtcgggggcg tccgcattgg cactggcgct tgtgctgggt ccgcggatcg
     1861 gattcaagtc cgaggcgatg cgaccacaca acctgccgtt cgtcctgctc ggggtcgggt
     1921 tgctgtggtt cggctggttc ggattcaacg caggttcggc gctcgccgcg gacggcaagg
     1981 cgtccgtggt cttcctcaac acgctgatcg cagggtgcct cgggatgatg ggctggatct
     2041 ttgtcgaacg tgtccgagac ggtcggccca cgaccttcgg agcggcatcc ggagtgatcg
     2101 cgggtttggt ggcgatcact ccgtcgtgcg gattcgtcgc accactcggc gcttttgcga
     2161 tcggcctggc ggcgggtgtg gtgtgttcct acgcagtcgg ttggaagttc aaggccggct
     2221 acgacgactc gcttgacgtt gtcggtgtgc acttcgtcgg cggcgtcgtc ggcaccttcc
     2281 tgatcggact gctggccgcc gaggtggtgt ccggcggagt cgaaggcttg ttctacggcg
     2341 gcgggctggc actacttggc aagcagactc tggccattcc ggtggtcgcg ctgtacgcct
     2401 tcatcgtttc gttcatcatc ggcaaggtca tcgaagccac attcggattc cgcatcgagc
     2461 gtgaggacga gatttccgga atcgatctcg cgcagcacgc ggagaccgcc tataccgaag
     2521 gcgtgcacgg cgcaccccag aagcggcccg gcatcggatg acttcattcg tcatcacact
     2581 gcggcagcgg cctctacggc ctcgatgcgg gcaggcacat gcttgtgcca cggtgtcccc
     2641 gcataagcgt cacgccattc cgtcgacgtc aatgcgttcg gtgcaacgcc gggaacgtgt
     2701 gtctgtcctt cgtcgtcgac gaaatcaagg ccgagcccgt tcggcaacga cgcgtggccg
     2761 ggaagcatcg cctcgctgat ctcgacgctc gcctcggcgc tgcccgccgc ggtggtgatg
     2821 cgggcgcgtc cacctgcgac cagaccgagt gactgcgc
//
LOCUS       DASYLX010000550         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3865821,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000550 DASYLX010000000
VERSION     DASYLX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865821"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VGP27_13285"
     CDS             complement(<1..677)
                     /locus_tag="VGP27_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7852305.1"
                     /translation="MPSIASRSLLPGWSIPVVFVIGAISQYVGAAIGVFLFETTEPAT
                     VAWLRAAAAAVALVGWRRPWRREWNRRQAGAALMFGLVTIGMNIAIYEAMARIPLGTA
                     VAIEFLGPTAVAAAGSRRPRDVAAVGLALAGVLLLAGVEFHTNLTGVLFALVSAAMWG
                     GYILLGKRVADTGDGLDTLAVGMSLGAVVLAPAILIPQAAINGSVFADPRTWLLGLCI
                     GLLSTAIP"
     gene            804..1763
                     /locus_tag="VGP27_13290"
     CDS             804..1763
                     /locus_tag="VGP27_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7852306.1"
                     /translation="MGATDEQRAGVKLTNLDQPLSQQAGATKRDLVDYLDAVADRMLP
                     GLAGRPLTVLRVLRDQKPFMQKNVPKYTPDWVRTVPIWAEASKREVHYALCDDRRTLL
                     WFANQRAVEYHPTLGLADDIYRPTHLVLDLDPPADDAFAAVVAVAHLVRRALADCGLA
                     GAVKTSGAKGIHVFVPIDDSAPVDDVAAATRALAARAEALDRDLATTAFIVEDRRGKV
                     FVDSTRAGGATVASAYSPRLRPGTPVSFPLDWSDLDDADPGDFTLHTAIDALGGRDPW
                     ADSMPQPQRLPTDLIEQGRAIPVARVAAMHEGKRRARARREKA"
BASE COUNT          338 a          661 c          667 g          269 t
ORIGIN      
        1 gggatcgccg tggagagcag gccgatgcag agacccagca gccacgtccg tggatcggcg
       61 aataccgagc cgttgattgc ggcctgtggg atgaggatcg cgggcgcgag aaccaccgcg
      121 cccaacgaca ttccgaccgc aagggtatcg agtccgtcgc cggtgtcggc cacccgcttg
      181 ccgagcagga tgtagccgcc ccacatcgcg gccgacacca acgcgaacag cacgccggtc
      241 aggttggtgt ggaactccac gccggccagc agtagcacac ccgccaacgc aagaccgact
      301 gcggcgacgt cgcgcggcct gcgcgatccc gccgcggcca ccgccgtcgg gccgagaaac
      361 tcgatggcga cggcggtgcc gagcgggatg cgcgccatcg cttcgtagat ggcgatgttc
      421 atgccgatcg tcacgagccc gaacatcagt gccgcaccgg cttgccgccg gttccactcg
      481 cgccgccacg gccgccgcca ccccacaagt gcgaccgcgg cggccgccgc tcgcagccac
      541 gccacggtcg ccggttcggt tgtctcgaac aggaagacac cgatggcggc gccgacgtac
      601 tgggagatgg cgccgatgac gaagacgacg gggatggacc aacccggcag cagggatcga
      661 gaagctatgg agggcaaccc tacgtcaggc cgtcaggcgg aaacccgccg ttgccgcgag
      721 aacagcacga gctccgacgc cgcgatgtag gagaacatca cggtgaagcc gaacgcgaag
      781 gtcaatgtgc ctaggctgat tggatgggtg ccaccgacga gcagcgcgcc ggggtgaaac
      841 tcaccaacct cgaccaaccg ctgagccagc aggcaggtgc caccaaacgc gacctggtgg
      901 actacctcga cgccgtcgcc gaccggatgc tgcccggcct ggcaggcagg ccgctcaccg
      961 tgctgcgggt actgcgggac cagaaaccgt tcatgcagaa gaacgttccg aagtacaccc
     1021 cggactgggt ccgcaccgtg ccgatatggg cggaggcgtc caagcgcgag gtgcactacg
     1081 cgttgtgcga cgaccgccgg actctgctct ggttcgccaa ccagcgagcc gtcgagtacc
     1141 acccgacgct ggggctggcg gacgacatct accggcccac gcatctggtg ctcgacctcg
     1201 acccgcccgc cgatgacgcg ttcgccgcag tggtggccgt ggcacatctg gtacgtcgcg
     1261 cgctggctga ctgcggactg gcgggtgcgg tgaagacgag tggcgccaag gggattcacg
     1321 tgttcgtgcc gatcgatgac tccgcccccg tcgatgacgt cgccgcggcc acccgtgcgc
     1381 tggcggccag ggcggaggcg ctcgatcggg atctggccac caccgccttc atcgtcgagg
     1441 accggcgcgg aaaggtcttt gtggactcca cccgcgccgg tggcgctacc gtggcgtccg
     1501 cctacagtcc gcgccttcgg ccgggcacgc cggtgtcgtt tccgttggac tggtcggatc
     1561 tggatgacgc cgaccccggc gacttcactt tgcacacggc gatcgatgcg ctcggcgggc
     1621 gtgacccgtg ggcggattcg atgccgcaac cacagcgcct gccaaccgat ttgatcgagc
     1681 aggggcgcgc cattccggtc gcccgcgtgg ccgcgatgca cgagggcaag cgccgcgccc
     1741 gcgcccgtcg tgagaaggcg tagcggctca ggcgacgagg tcctcggcgg cggggcttgc
     1801 acaggacacg caggcctctt cctcgaggct gaagtggccg caggtcgggc agatgaagtc
     1861 gagcatggcg ggccgccttc ccaggctgaa aacgaagggg ttgacgccga ccttctgcag
     1921 cgctgccagc ctgtt
//
LOCUS       DASYLX010000551         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_355312,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000551 DASYLX010000000
VERSION     DASYLX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355312"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGP27_13295"
     CDS             <1..330
                     /locus_tag="VGP27_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852307.1"
                     /translation="DDGVSLDTFVDWLIEAGHPIARIEDHDDWLHRFETALTALPDAQ
                     RRHSVLPLLDAYRKPERPILGAIAPADAFRAAVRAAGVGAEGDIPHITQSLIGKYVSD
                     LQRLDLV"
     gene            357..1112
                     /locus_tag="VGP27_13300"
     CDS             357..1112
                     /locus_tag="VGP27_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897510.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV7852308.1"
                     /translation="MPIAARAKLANRLMVLAAAGMAVAGLSACDARSTAGPSMIPTAD
                     RDTRQVTVVGSGEVQGKPDTLNANVSIEATAPDVTAAMNQTSDRMQAVINALTNSGID
                     RNDIATTNVTVQPQYSGGDDPTITAYQASNSIGVKIRDLNNASQTLGLIGTTGGDATR
                     INSVSYAIDDDSQLVKDARTRAFDDAKDRAEQYAQLAGLTLGDVISISESAGTMPPTP
                     MPAPMPRAAMEAVPLEPGQQSVSFSVTVIWELT"
     gene            complement(1137..>1679)
                     /locus_tag="VGP27_13305"
     CDS             complement(1137..>1679)
                     /locus_tag="VGP27_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV7852309.1"
                     /translation="ERLKAAPHGIDLGPLRPRLPEVLRTPSGRIELAPEPFTVDAARL
                     REQLGRDADRFVLIGRRHLRSNNSWMHNVPALAGGTNRCTLQMHPDDIAELALSDTAK
                     VKGPGGEVLAPVEPMPGMRRGVVSLPHGWGHDRGGTRLGVAAGRPGVNVNQLNDGSHL
                     DPLSGTAVLNGIPVDIAPAD"
BASE COUNT          289 a          593 c          551 g          246 t
ORIGIN      
        1 gacgacggcg tatcgctgga cacgttcgtc gactggctca tcgaagccgg tcacccgatc
       61 gcacgcatcg aggatcacga cgactggctg caccggttcg agacggcact gaccgcgctg
      121 cccgatgccc agcgacggca cagcgtgctc ccactgctcg acgcgtatcg caagccggag
      181 cggccgatcc tgggcgccat cgcaccggca gacgcgttcc gcgccgccgt gcgtgcggcc
      241 ggcgtcggtg ccgaagggga catcccccac atcacgcagt cgctgatcgg caagtacgtg
      301 tctgacctcc agcgcctcga cctcgtctga tcgagaggtg ctctaggctg ccaggcatgc
      361 cgatcgcagc gagagcgaaa cttgcgaacc gattgatggt cctcgccgcg gcggggatgg
      421 cagttgccgg cctgtcggcc tgcgacgcga ggtcgacggc cggaccgtcg atgattccca
      481 ccgccgaccg ggatacccgc caggtcaccg tcgtcggatc cggtgaggtg cagggcaagc
      541 cggacacgtt gaacgcgaac gtctccatcg aagccaccgc gcccgacgtc accgccgcca
      601 tgaaccagac cagcgaccgc atgcaggccg tgatcaacgc gctgacgaac tccggcatcg
      661 accgcaacga catcgccacg acgaacgtca ccgtgcagcc gcagtacagc ggcggcgacg
      721 acccgacgat caccgcctac caggccagca actcgatcgg cgtcaagatc cgcgacctca
      781 acaatgcgtc gcagacgctg gggctgatcg gtaccaccgg cggcgacgcc acccgcatca
      841 actcggtcag ctacgccatc gacgacgact cgcaactggt gaaggacgcg cgcacgcggg
      901 ccttcgacga cgccaaggat cgcgccgagc agtacgccca attggccgga ctcactctgg
      961 gcgacgtcat ttcgatctcc gagtcggcgg gcactatgcc gccgacgccg atgcctgcgc
     1021 ccatgccgcg ggcggccatg gaggccgtgc cgctggagcc gggccagcag agcgtcagct
     1081 tctcggtgac ggtgatctgg gagttgacct agcggcacgc gggcggcatg acgaattcag
     1141 tccgcggggg cgatatcgac cgggatgccg ttgagcacgg ccgtgccgga cagcgggtca
     1201 agatgactac cgtcgttgag ctgattgaca ttgacgccgg gccggcccgc cgccaccccg
     1261 agccgcgtgc caccgcgatc gtgaccccat ccgtgcggta gcgacaccac accgcgccgc
     1321 atccccggca tcggctcgac gggagccagg acttcgccgc cgggcccctt gaccttcgcc
     1381 gtgtcggaca gcgccagctc ggcgatgtcg tcgggatgca tctgcagcgt gcaccggttc
     1441 gtaccgccgg ccaacgcggg cacattgtgc atccagctgt tgttggagcg cagatggcgt
     1501 ctgccgatca ggacgaacct gtcggcatcc ctgccgagct gctcgcgaag ccgcgcggcg
     1561 tccacggtga acggctccgg cgcgagttcg attcgcccgc ttggtgttcg gagcacttca
     1621 ggcaaccggg gtcgcagcgg tcccaggtcg atgccgtgcg gtgcggcctt gagccgttc
//
LOCUS       DASYLX010000552         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1673155,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000552 DASYLX010000000
VERSION     DASYLX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673155"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VGP27_13310"
     CDS             complement(<1..216)
                     /locus_tag="VGP27_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852310.1"
                     /translation="MNDSTARNLTRFKYAATLLGAMAAFALAPAIAHAQPVGGGSGGG
                     CHYTDKDGYDIPIDDGQSVLVDGKTVSC"
     gene            795..1436
                     /locus_tag="VGP27_13315"
     CDS             795..1436
                     /locus_tag="VGP27_13315"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HEV7852311.1"
                     /translation="MPGPLDKALLGGAPQRVGTFEYRYDTDTWSWSDTVAELHGYEPG
                     GSVQPTTDLVLSHKHPDDLAEVKGLLKQSAAPFSSRHRIYTTTGEERKVVVVGDAVFD
                     EDHQIVATRGFYIDITAVTEADVQDAISEELEAIVSNREVIEQAKGMLMMLYELDADA
                     AFAVLRWRSQQLNVKLHTVAADLVQDLPPLVQRTSEDRRAADHYLITAPAPDV"
     gene            1829..2020
                     /locus_tag="VGP27_13320"
     CDS             1829..2020
                     /locus_tag="VGP27_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852312.1"
                     /translation="MGRVIVIIVGVVVALFGLLFALQGFGVVGGSPMSNTATWSILGP
                     IIALVGIAIAAGAARRRKT"
     gene            2064..>2260
                     /locus_tag="VGP27_13325"
     CDS             2064..>2260
                     /locus_tag="VGP27_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852313.1"
                     /translation="MSAVDGLPPRRRIDELTAARSDSWRSMALTSRCFTTPQSPVTRM
                     RQSIVGAETSLRRYWIRRADD"
BASE COUNT          421 a          701 c          696 g          442 t
ORIGIN      
        1 gcacgagacg gtcttcccgt cgaccaagac agactggccg tcgtcgatcg ggatgtcgta
       61 accgtccttg tcggtgtagt ggcatccgcc accgctaccg ccaccgacgg gttgcgcgtg
      121 agcgatcgcc ggggccaaag cgaaagcggc catggcgccg aggagcgtgg ctgcgtactt
      181 gaagcgggtc aggttgcggg cggttgaatc gttcatcatg gttcctctcg ttggggtgtt
      241 gagagcaaca atgccgattt gaggggtgct tccgtaatgg cccacaagcc agattccacc
      301 gttgaaaggg aagggtttcc ttgccctgag gcaaggcgct gaggccgcct tacctcgacc
      361 aggggttact cgggccgcct tgaactccaa acgtcgtggg ccctggcgca gtccgaatgt
      421 gaccaagaac gctccgacgt ttgccatttc tgcgcgcact gtgggtatac ggcggctatg
      481 caagttcacg ttctcctcga ggccttagct ggagtcgttg ggttcgcggc ggcagcgggg
      541 gtcggagtat gcatggtctt gtgtgctcgt agccatttca cccaggtttg ggatcacgtc
      601 cgacgtttcc tgaacgacta gccacgacag cgcgccgacc ggtggtggaa ggcggacgaa
      661 ggcctcacat tcccgacgcg gttcgcgaac gtggccttta tattttgtcc atgaccgaca
      721 acccggctat tcggcgacgc tcttcatttg tgcgggcatc ggagtttggg tctcccaccg
      781 gtaccgacgg gtacgtgccg ggcccgttgg acaaggcgct gctgggtggc gcgcctcagc
      841 gcgtgggcac attcgaatac cgctatgaca ctgacacatg gtcgtggtcg gacacagtcg
      901 ctgagctgca cggctatgag ccgggcggct cggtgcagcc aaccaccgac ctcgtcttga
      961 gccacaagca cccagacgac ttggccgagg tgaagggcct actcaagcag tcggccgcgc
     1021 ccttcagcag tcgccaccgc atatatacga cgaccggcga ggaacggaag gtcgtcgtgg
     1081 tcggcgacgc cgtcttcgac gaggatcacc agatcgtcgc tacccgcggc ttctacatcg
     1141 atatcaccgc cgttaccgaa gccgatgtcc aggatgcgat cagtgaagag cttgaagcaa
     1201 tcgtttccaa ccgcgaagtg atcgagcagg cgaagggcat gctcatgatg ctttacgaac
     1261 tcgatgccga cgccgcattc gccgttcttc gctggcgatc acagcagctc aacgtcaagc
     1321 tgcatacagt cgccgccgac ctggtccagg acctaccgcc tttagttcaa cgcacctcgg
     1381 aagatcgcag agcggctgac cactacttga tcacagctcc cgcgcccgac gtctaagcgc
     1441 aaggctgcat ccaaacttct caaccgtcag ccgttctcgc cgaggacgct gccgttgcca
     1501 acccgggtga tcgtgcacgg cgaaccattc cagtcatagc gtgcaactgc gagcggtatg
     1561 cgcttgttgt gcgtgcttaa cggcttacct ttttgggtgc gcaaccgaag aacacggata
     1621 ccaaaccact ccagttcgac ttgtcaccaa cggatttgcc gtagcgattg aattcgcgga
     1681 cgccacctcg tggattgatt tcactatcgt tgcgcgcgca gaacgccagc caccgcgcgc
     1741 taccggcaag gtcggacgcc agccatctga acgttcgtac gcaacgcgcg gccgggaaac
     1801 aaccggcttt cgcggctact gtctagccat gggacgcgtc atagtcatca tcgtgggcgt
     1861 tgttgtcgcc ctcttcggcc tgctgttcgc gctacagggc ttcggggtgg tggggggcag
     1921 ccccatgagc aacaccgcca cttggtcgat cctgggtccc atcatcgcgc tggtcggaat
     1981 cgcgatcgcc gccggcgcgg cccgcaggcg caagacctag cggcggtcgc cggctggcga
     2041 tccgctcgtc gagcaactca tcgatgagcg cagtcgacgg ccttccgccg aggcggcgga
     2101 tcgacgagct cacggcagcc agaagtgaca gctggcgatc gatggcgctg acgtcgcggt
     2161 gtttcactac tccccaatca ccggtaaccc ggatgcggca atcgatcgta ggcgccgaaa
     2221 cgtcgcttcg ccggtattgg atccggcgtg ccgacgacat
//
LOCUS       DASYLX010000553         5221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1123726,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000553 DASYLX010000000
VERSION     DASYLX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123726"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1519
                     /locus_tag="VGP27_13330"
                     /pseudo
     CDS             243..1519
                     /locus_tag="VGP27_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891169.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1595..2329
                     /locus_tag="VGP27_13335"
     CDS             1595..2329
                     /locus_tag="VGP27_13335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010843884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852314.1"
                     /translation="MTPESVIAVTGASSGIGEATARHLATRGARLVIGARRTDRLEAL
                     ADEIRKDGGEVVPVEVDVTRRADLQRLVQTAVDRFGRLDVLVSNAGVSKLGPLADGDV
                     DGWSAMIDVNLRGVLNGIAAAMPVFRRQGNGHFVTTVSTAGLKIVPDMAVYAATKNAV
                     RTVMESLRQESTDGVIRTTSVSPGFVRTELGDSIDDPDARARIRSNMDAIGLSPEAVA
                     RAVAFAIEQPRDVEIGEIVIRPTVQN"
     gene            complement(2391..3389)
                     /locus_tag="VGP27_13340"
     CDS             complement(2391..3389)
                     /locus_tag="VGP27_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7852315.1"
                     /translation="MNFIEKFRGKWGRRLAAGALVATALPALIGFAGGSATAGAFSRP
                     GLPVEYLDVFSPSMNRNIRVQFQGGGPHAVYLMDGLRAQDDYNGWDVNTPAFEWYYGS
                     GLSTVMPVGGMSSFYTDWYRPSQGNGQDYTYKWETFLTQELPVYLEANQGVSQNGNAV
                     VGISMAGSTALTYSIYYPQKFIYAASLSGFLNPSEGWWPMLIGLAMNDAGGYSAEDMW
                     GPSSDPAWKRNDPMVNVNQLVANNTRLWIYCGNGTPADLDVGSDGGNLMAAQFLEGFT
                     LRTNEAFRDTYVAAGGQNAVFNFPASGTHSWGYWGQQLEQMKPDLQRVLGASPTSA"
     gene            complement(3562..4791)
                     /locus_tag="VGP27_13345"
     CDS             complement(3562..4791)
                     /locus_tag="VGP27_13345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV7852316.1"
                     /translation="MDMDRRIPKPRDLAPLIQFKKPTRNRTARRLSDALTIADLRRIA
                     KRRTPRAAFDYTDGAAEEELSLARARQAFLDIAFHPTILRNVAEIDTGWDVLGDRVAQ
                     PFGIAPTGFTRLMQTEGEIAGAHAAARAGIPFSLSTLGTRSIEEVKAANPNGRNWFQL
                     YMWKDRDRSMALVDRAAAAGFDTLLVTVDVPVAGARLRDTRNGMSIPPALTLRTVLDA
                     VPHPQWWFDLLTTEPLSFASLDRWPGTVAQYLDTMFDPTVTFEDLAWIKSQWPNKLVV
                     KGIQTLEDAKSVVDLGVDGIVLSNHGGRQLDRAPVPFHLLPTVAREVGGDTEIVLDTG
                     IMSGADIVAAIALGARFTLVGRAYLYGLMAGGEAGVDRAIAILSEQVARTMRLLGVTS
                     LAELTPHHVSRLTGHSE"
     gene            complement(4788..>5221)
                     /locus_tag="VGP27_13350"
     CDS             complement(4788..>5221)
                     /locus_tag="VGP27_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7852317.1"
                     /translation="RMADALRAIAPTLRLTHTDQQVLAAHARITRYGADEYIQFAGQV
                     PEQMTFLVKGHVRLIARGEDGLVIPIRTLEEGDFIGQTALTREPVVASAYAIDEVTAL
                     QIQRDYIAELVERKPLLLQDIGRAIEERRAYVRRAITAVAR"
BASE COUNT          904 a         1688 c         1726 g          903 t
ORIGIN      
        1 tgggcagcac gatgggctcg ctcaagaaaa ggacgggcac cgcagtcgtc acgcttgttg
       61 cttcaggcat tcatctcctc caaagttcag cctgttgcac tcaaccttgt gaggggctcc
      121 tttgttcccg gcgagcgccg aatttcggcc ccatcaggct ggccgcaggt gagacgtccg
      181 ccccccgcgc gaccacccat ctgggcgatg tccggccacc gctgtgccag gtactcacgt
      241 gcatgccccc ttggcgactg cgtgacttcc acgaggacga cctcgaccag gccatcggcg
      301 tctgggacca gagccgaagc cgtgacgagg cacatccggt gttcggagtg tcggaggtga
      361 tttcggcggc caggtccggc cagccggcgg tggtcgccgt catcggcgac gatcttgtcg
      421 gcatggcggc cgcgcaggcg cacggggagc gagcgtggat ctccgtagta gcgctgagca
      481 atctttggcg gaatggcggc atcggaagtg cgctggtcgg cgaattggag atccggctgc
      541 gcacgatggg ggtgcggcgg atatgtgcgc ttctgcccgc ggatgcaacg gggacggcgg
      601 ccctgcagaa ctcgggttac gccgaacgca ccgcattgag ctatttcgaa aagctcgatc
      661 atgtcggcac cgcagatgcg ggtttgctgg ccaacctcgg cggtcaggtg atggccggtg
      721 gactctggca cgcgatggcc ggaatggaag gcgagaagca actcatcgag aagcgcatcg
      781 tgctgcccct cgtcgaaccg aataccgctg cgcagtacgg tgtttcgccg ccgaaggcgg
      841 ttatcctgta cggcccgccc ggcacgggga agacaagttt cgccaaggcc gtcgcaggtc
      901 ggctgggttg gccgttcgtc gaactgttcc cgtcacggct cgcgactcct gacgtcgcga
      961 tggccagcgc actgcgcgag gccttcgcaa cgctgatgga attggaggcc gccgtggtct
     1021 tcatcgacga agtagaggag attgcaggca gtcgctcggg cgccccctca gatccctcac
     1081 atggtgtgac gaacgaattg ctcaagctga taccgcgttt cgccagcaag gcgggcgcct
     1141 gctggtgtgt gcgacgaact cagtgcgatc gctggactcg gcgttcttgc ggccaggcag
     1201 atttgactgc atcatcccga tcggaccgcc ggatgccgtt gcccgcaccg ccatttggaa
     1261 tcgctacctg ggcccgatga cggatgcagt agaggtcgac acattggtcg aggcaagcgc
     1321 gatgttcacg cccgccgaca tcgaattcgc cgcacgcaag ggcgcccagt gcgcattcga
     1381 acgcgaggtc gagttccggc gcggggagcc cgctcgcacc gacgactacc tgaccgccat
     1441 cgccgatacg cgtccgacgt tgaccgacgc gatgctgtcc gagttcgaag aagacattgc
     1501 gctgcgtact cggttgtgac ccggtgacgg ggcgtgcggg catccgtcgg ggaatagccc
     1561 ctcgacgaca taggtcgtag ttgccatggt cgacatgaca cccgaatctg tcatcgccgt
     1621 caccggagcc agcagtggca tcggggaggc gaccgcgcga cacctggcta cgcgtggagc
     1681 ccgtctggtc atcggcgcgc gtcgaacgga ccgactggaa gccctcgcgg acgaaatccg
     1741 caaggacggc ggggaagtag tcccggtcga ggtggacgtc actcgtcgcg ccgacctcca
     1801 gcggctggtg cagacggcgg tcgaccggtt cggccggctc gacgtgttgg tgagcaacgc
     1861 gggcgtcagc aagctgggac cgttggccga cggcgacgtg gacggctggt cggcgatgat
     1921 cgacgtgaac ctgcggggcg tgttgaacgg catcgccgcc gccatgcccg tgttccggcg
     1981 acagggaaac ggccacttcg tgacgacggt gtccacggca gggctgaaga tcgtcccgga
     2041 tatggccgtc tacgccgcca ccaagaatgc ggtccgcacg gtgatggagt cgctgcggca
     2101 ggagtccacc gacggtgtca tccggaccac ctcggtgtcg cccgggttcg tccgcaccga
     2161 actcggcgac tccatcgacg acccggacgc ccgcgcccgc atccggtcga acatggacgc
     2221 catcggcctc tcgccggaag ccgtggcacg ggcggtcgcg ttcgcgatcg aacagccgcg
     2281 cgacgtcgag atcggtgaaa tcgtcatccg cccaaccgtt cagaactagg cccaaaagca
     2341 gtgagcctgc cgccaggggt agcggcaggc ccactgaagg ggaggtgctg ttacgcggag
     2401 gtgggggacg cgcccagcac ccgttggaga tcgggcttca tctgctcgag ctgctgtccc
     2461 cagtagcccc agctatgtgt gccactcgcg gggaagttga acactgcgtt ctgcccgcct
     2521 gcggccacat aggtgtcccg gaaggcctcg ttggtccgca atgtgaaccc ctcgaggaac
     2581 tgagccgcca tcaggttgcc gccgtcgcta cccacgtcga gatccgcggg cgtgccgttg
     2641 ccgcagtaga tccagagcct ggtgttgttg gcgaccaact ggttgacgtt gaccatcggg
     2701 tcgttgcgct tccaggccgg atccgaggac gggccccaca tgtcctctgc gctgtagccg
     2761 cccgcgtcat tcatcgcgag accgatcagc atcggccacc aaccctcgga ggggttgagg
     2821 aagccggaca gcgatgccgc atagatgaac ttctgcgggt agtagatcga atacgtcagc
     2881 gcggtgctgc ccgccatcga gatgccgacc acggcgttgc cgttctgcga caccccttgg
     2941 ttggcctcca gatagaccgg cagctcctgg gtcaggaagg tctcccattt gtacgtgtag
     3001 tcctgaccgt tgccctgcga cggccggtac cagtcggtgt agaagctcga catgccgccg
     3061 accggcatca ccgtcgaaag gccggagccg tagtaccact cgaacgccgg ggtgttgacg
     3121 tcccaaccgt tgtagtcgtc ctgcgcccgc aggccgtcca tcagatacac cgcgtgcggt
     3181 ccaccgccct ggaactgaac cctgatgttg cggttcatcg acggcgagaa cacgtccaga
     3241 tactcaaccg gcagacccgg gcgggagaac gcgcctgcag tcgccgaccc cccggcgaaa
     3301 ccgatcagcg ctggcagggc cgtcgcgacg agggcgccgg ccgccagtcg gcgtccccac
     3361 ttacctcgaa acttctcaat gaaattcata acggcaacca acccaccttt ccaattactt
     3421 gctccaaagc attgccgttg ctttgcgtga gttagtgaaa catccgccac atccgtcaca
     3481 ggcgcaccaa cacggcgttc cgatatcgcg aatggctaac gattgcgact cggcgccgat
     3541 atcaagccgc cgaactgggc tctactcgct gtggccggtc agccggctga cgtgatgcgg
     3601 cgtcaactcc gccaaagacg tcacgccgag caaccgcatg gtgcgcgcga cctgctcgga
     3661 gaggatcgcg atggcgcggt cgacaccggc ctcaccgccc gccatcaggc cgtagagata
     3721 cgcacgcccg accagggtga accgtgcgcc gagggcgatg gcggcgacga tgtccgcgcc
     3781 ggacatgatc ccggtgtcca gcacgatctc ggtgtcgccg ccgacttcac gcgcgacggt
     3841 gggcaacaga tggaacggca ccggcgcccg gtcgagctga cgtccgccgt ggttcgacag
     3901 cacgatgccg tcgacgccga ggtcgaccac cgacttggca tcttccagtg tctgaattcc
     3961 cttgaccacc aacttgttcg gccactgcga cttgatccag gccaggtctt cgaacgtcac
     4021 cgtggggtcg aacatcgtgt cgaggtattg ggcgacggtg ccgggccagc ggtccaacga
     4081 ggcgaacgac agcggttcgg tggtcagcaa gtcgaaccac cactgcgggt ggggcactgc
     4141 atcgagcacc gtacgcaggg tgagggcggg cggtatcgac atcccgttgc gggtgtcccg
     4201 aagccgtgcg cctgcgacgg gaacgtcgac ggtgacgagc agggtgtcga accccgccgc
     4261 ggcggctcgg tcgaccaagg ccatcgagcg gtcccggtcc ttccacatgt atagctgaaa
     4321 ccagttgcgt ccgttagggt tagctgcctt gacctcttcg atcgacctgg taccgagcgt
     4381 cgacaacgag aaggggatgc ccgcccgcgc cgcggcgtgt gcacctgcga tctcaccctc
     4441 ggtctgcatc aaccgcgtga agcccgtcgg cgcgattccg aacggctgcg ccacccggtc
     4501 gcccagcacg tcccatccgg tgtcgatctc ggcgacgttg cgcagaatcg tcgggtggaa
     4561 tgcaatgtcg aggaaggcct gccgcgccct ggcgagcgac agttcctcct ccgcggcgcc
     4621 gtcggtgtag tcgaaggccg ctctgggagt gcgccgcttg gctattcgtc gcaggtcggc
     4681 gatcgtcagc gcatcggaca gcctgcgggc cgtgcgattg cgcgtcggtt tcttgaactg
     4741 gatcagcgga gccagatcgc gcggcttcgg tattcgacgg tccatgtcta tcgcgccact
     4801 gcggtgatcg cgcgtcgcac gtacgcccgc cgttcctcga tcgctcggcc gatgtcctgc
     4861 aacagcaggg gcttccgctc gaccagctcc gcgatgtagt cgcgttggat ctgcaacgcg
     4921 gtcacctcgt cgatcgcgta ggcgctcgcc acgaccggct cgcgggtcag cgcggtctgg
     4981 ccgatgaagt cgccctcctc cagcgtgcga atgggaatga ccaggccgtc ctcgcccctg
     5041 gcgatgagcc ggacgtggcc cttgaccagg aacgtcatct gctccggaac ctgaccggcg
     5101 aattggatgt actcgtcggc gccgtagcgg gtgatgcgcg catgagcggc gagcacctgc
     5161 tggtcggtgt gggtcaatcg tagagtcgga gcgatggcac gcagcgcatc ggccatccgg
     5221 t
//
LOCUS       DASYLX010000554         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2988840,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000554 DASYLX010000000
VERSION     DASYLX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988840"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGP27_13355"
     CDS             <1..138
                     /locus_tag="VGP27_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852318.1"
                     /translation="ALTAMRRSIDAADVTGADVAKARETVRPSLDPAQVESLREFSAA
                     R"
     gene            complement(143..1195)
                     /locus_tag="VGP27_13360"
     CDS             complement(143..1195)
                     /locus_tag="VGP27_13360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852319.1"
                     /translation="MAVDEIKLNEFLGKAVGDLGAAMSATLILVGDRLGLYRELATGA
                     MTSTELAQRTGTDERYVREWLGNQGAGGYVQYDPASNEWSLSPEQALCLTDPDGPVDM
                     PGAYNIVEATFHALEKTLDNFRSGDGMEWGDHHPCLFEGTERFFRAGYNTNLIGSWLP
                     ALDGVVDKLKTGAKVADVGCGHGASTILMAMSYPDSEFIGYDYHDDSIKVATEKAAAA
                     GAANARFEVADAVGYQGKDFDLIAFFDCLHDMADPVSVSRHAREAISDDGTAMIVEPF
                     ANDAVADNLNPVGRVMYGASTQICVPVSLARNGPALGAQAGEARIREVVVDGGGFTRF
                     RRATETPFNLVLEARP"
     gene            1614..2099
                     /locus_tag="VGP27_13365"
     CDS             1614..2099
                     /locus_tag="VGP27_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEV7852320.1"
                     /translation="MTAPITSTRAGDREREKTAGQLGSALAHGYLQMDEYETRLQSAF
                     AAHTTAELRQLVADLPLGQLRRHDPRRREARRSAARRGVRMHLAGYLAMVVVVLTVWL
                     AVGLTAGTWYFWPIWPILGAGIGVIGHAMPIRFATGRPMASAPLDHCGQAPTGYRGSG
                     V"
     gene            complement(2049..>2700)
                     /locus_tag="VGP27_13370"
     CDS             complement(2049..>2700)
                     /locus_tag="VGP27_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7852321.1"
                     /translation="QAEHYREGRVFIAGDAAHVHSPLGGQGMNTGIGDAMNLGWKLSS
                     AVRGTAPNWLLDSYEGERHPVGAAVLRLTDAFNQLVLGRSRVQRLLQTIAVATITRIP
                     AGRRFMAGRLSQLGIGYPRRSRGDHRMVGKRMPDVDCGGTRLYELLRGGRFVMLTTDD
                     VDIDRPDVVHARHHDTELPAAVLVRPDGYVAWVDDRPPTKSHAAAAIARWCLPAVV"
BASE COUNT          450 a          930 c          858 g          462 t
ORIGIN      
        1 gccctgacgg cgatgcggcg atcgatcgac gccgcggacg tgaccggcgc cgacgtggcc
       61 aaggcccgcg aaaccgtgcg cccctcgttg gacccggcgc aggtcgagtc gctgcgcgaa
      121 ttctcggccg cccgctgagt agttacggcc gcgcctccag cacgaggttg aacggcgtct
      181 cggtggcccg ccggaaacgg gtgaagccgc caccgtcgac gacgacttct cgtatccgcg
      241 cctcgcccgc ctgcgcaccg agtgcaggtc cattgcgcgc cagggagacc ggcacgcaga
      301 tctgagtgga cgcgccgtac atcacgcggc ccaccggatt cagattatcg gccaccgcgt
      361 cgttggcgaa cggctccacg atcatcgcgg tgccgtcgtc gctgatcgcc tcgcgcgcgt
      421 gccgcgacac gctgaccgga tcggccatgt cgtgcaggca gtcgaagaac gcgatgagat
      481 cgaagtcttt gccctggtag ccgacggcgt cggcgacctc gaatcgcgcg ttcgccgcgc
      541 ctgccgccgc ggccttctcg gttgcgacct tgatcgaatc gtcgtgatag tcgtaaccga
      601 tgaactccga atccggatag gacatcgcca tcaggatcgt gctggcgccg tgcccacacc
      661 cgacatcggc aaccttggct ccggttttca gcttgtcgac gacaccgtcg agggccggca
      721 gccacgagcc gatcagattc gtgttgtatc cggcccggaa gaatcgttcc gtcccctcga
      781 acaggcatgg gtggtggtcg ccccattcca tgccgtcacc cgacctgaag ttgtcgagcg
      841 tcttctcgag agcgtgaaac gtcgcttcga cgatgttgta tgcgccgggc atgtcgaccg
      901 gcccgtccgg gtcggtcagg cacagcgcct gctccgggct cagcgaccac tcgttggatg
      961 cggggtcgta ctgcacatat ccgcctgcgc cctgattgcc gagccattcg cggacgtaac
     1021 gttcgtcggt gccggtgcgc tgcgccagct cggtggaggt catcgcgccc gtggccagtt
     1081 cgcggtacag cccgagtcgg tcaccgacaa gaatcaacgt cgcgctcatc gcggcgccga
     1141 gatcacccac cgctttgccg aggaactcgt tcaacttgat ctcgtctaca gccatgggcc
     1201 catagtgacg tccgagcgac agcggtgtcg gacattggca cttaaagccc tactacggca
     1261 ggttcgcagc aaaccaatgc ttcccccgat cgggggacaa ccgaatcacc ctggtgatcg
     1321 tccggtcgcc cgacatacat caaacctgca tggcggcatt ctcaaatcat cgaaagacca
     1381 ctcacacagg agaattccga tgatcaagaa tgtcttcgcc cgctacctcg tccttctgtt
     1441 cgtcggtgga ggtgctctcg ggggcgccgc cgtcggcctt gcgagcatgg ccggcgccgc
     1501 gtaaaggccg agcgcctgtc ggtggttgca atacatcttg acagtgacta gttagaagcg
     1561 catagtcgga catataacta gtcaccgtca agatctagga gcagggaggc accatgaccg
     1621 ccccaatcac gtcgacccgt gccggtgatc gcgagcggga gaagaccgcg ggccaactcg
     1681 gctccgcgct cgcccatggc tatctccaga tggacgagta cgagacccgg ctgcagtcgg
     1741 cgttcgccgc gcacaccacc gccgagctgc gccaattggt cgccgaccta ccccttggac
     1801 aactgcggcg ccacgaccca cgccggcgcg aggcacgccg gtccgccgca cgccgcggtg
     1861 tgcggatgca tctggccggc tacctggcga tggtcgtcgt cgtgctcacg gtgtggctcg
     1921 ccgtcggcct gaccgcgggc acctggtact tctggccgat ctggcccatt ctcggcgcgg
     1981 gtatcggagt catcggccac gccatgccga tccgctttgc gaccgggcgc ccaatggcat
     2041 cggccccgct agaccactgc gggcaggcac caacgggcta tcgcggcagc ggcgtgtgac
     2101 ttggtcggcg gccggtcatc cacccatgcg acgtagccgt ccggacgcac cagcacggcc
     2161 gcgggaagtt cggtgtcgtg gtgtcgggcg tgcacgacgt cggggcggtc gatgtccacg
     2221 tcatcggtcg tcagcatgac gaaccggccc ccccgcagca gttcatacag ccgggtgccg
     2281 ccgcagtcga cgtccggcat gcgcttgccg accatgcggt ggtcgccgcg ggatcggcgg
     2341 ggatagccga tgccgagctg gctcagccgt ccggccatga accgacggcc tgcgggtatc
     2401 cgcgtgatcg tcgcgaccgc gatggtctga agcagacgct gcacccgaga acggccgagc
     2461 accagctggt tgaacgcatc ggtcagtcgc agcaccgccg cgccgaccgg atgccgctca
     2521 ccctcgtagc tgtccaacag ccaattcggt gccgttccgc ggacggccga cgacagcttc
     2581 cagccgaggt tcatcgcgtc gccgatgccg gtgttcatcc cctgtccgcc cagcggcgag
     2641 tgcacatgag cggcgtcacc cgcgatgaac acccggcctt cgcggtagtg ctcggcctgc
//
LOCUS       DASYLX010000555         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3207812,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000555 DASYLX010000000
VERSION     DASYLX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207812"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGP27_13375"
     CDS             complement(<1..257)
                     /locus_tag="VGP27_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7852322.1"
                     /translation="MTLIAVEEHVLPSDLINEVWPKPTTPESLTAKLVDVGEQRLRAT
                     DDAAPGTQQVSAGHTDLSRAINDRCAEIVTAHPLRFNALATL"
     gene            complement(254..1291)
                     /locus_tag="VGP27_13380"
     CDS             complement(254..1291)
                     /locus_tag="VGP27_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852323.1"
                     /translation="MTSSSSGPARVDLHHHLFPSGKLAQFVQRGLIANSGWRFPGGSP
                     HWTPETSLAFMDGLGIQTAVLSLPSDLESGLPAPERRAFAREVNTIARQTVDDHPGRF
                     GFFAHLPTPTDVDAALDELTYALDHLDADGITLTNVYGTGEEARSLGDDIFEPLWAEL
                     DRRHALVFLHGEQTPGRNRMPNRFLPTPVSEVPNETYKAAADLVTSGRKRQYPNTRII
                     LSHSGGSTPFLAARVAVLSHYVGCELSPEEIIADFRTFYFETALSGFETNLVALENFV
                     PPEQILFGTDFPAVSPQMAGWYTTNVDAYFRDRHDMLAQVMHGNAHTLLPRLSVNTAA
                     SAAAAPMKRSP"
     gene            complement(1373..2518)
                     /locus_tag="VGP27_13385"
     CDS             complement(1373..2518)
                     /locus_tag="VGP27_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7852324.1"
                     /translation="MTIRVAVWGPGSMGVIALRGVIDHPELELVDLVVHSDVKAGRDA
                     GELCGIDPVGVVATTDPGALVSGDADVVVYAAAANLRPLEAVNDMVSILRAGKNVVSC
                     SVVPLVYPNAVDPAFTDPLREAALAGGGSFFTTGIDSGFANDVLPLALSGVARTIESI
                     RVTEMFNYATYPDASAVYDILGFGKPPEEVPFAATPGVFTFGWGPVLHQLADGLEVGV
                     DDIKETVERIPADISFDTPTGHIAAGTIAAMRSTLTGYVEGKPTLVIDHVSRMYDDIA
                     PDWPQPHISIQPRDLGYGGASGRGVYRVEIEGSPAMRCEFEMADDHDHDLGARMAGAS
                     RMVNAIPAVCAAKPGLLSALDLPLITGKGLVRGLSGPSPDSRLVGSL"
     gene            2622..3467
                     /locus_tag="VGP27_13390"
     CDS             2622..3467
                     /locus_tag="VGP27_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7852325.1"
                     /translation="MNVLDALAASSNGLTSAQLSRQCGISTSTCALVLGELECGGWVA
                     RYGDRRYGLGSGLFGLVRGLRNQFPLLDQGRTALVDLHESLAAGCSMSRIDDDHLTVI
                     DAVGHGSDGEHVVGQRFPIDPPFGLVAMAWRDDAEVERWLRGVTPRLAQADIDQHRQV
                     LADVRARGYGAWRFDDAHASLHDRVAGILASLEPTASAARQLTKLMTIVTLRSITSTL
                     ETDLLATEFVVLPIFGPTGQPEYQIEIHISRPDALTLDSLNTALENAQNELAPTGTHA
                     GVQKV"
     gene            3701..>4901
                     /locus_tag="VGP27_13395"
     CDS             3701..>4901
                     /locus_tag="VGP27_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7852326.1"
                     /translation="MSTDPREERLARRITGLYATDPQFADARPIEALTAAIEQPALRL
                     TGIVRTVMSAYAERPAVGERAVNLATDPETGRTSLELLPRFETVTYGALWERVNAVVR
                     ALADGGVRPGDRVCVLGFTSVDYATIDMALGQLGAVSVPLQTSAPVSQLRPIVAETEP
                     RVLASSVYDLGDAVELVLTGHVPGRLVVFDYRPEVDEQREAFDAARSRLAEAGSPLVV
                     ETLAELLERGESLPAAPEFVPDDDDDDPLRLLIYTSGSTGAPKGAMYPERLVANFWRR
                     SRWNWGAPSVEPTITLSFMPMSHVMGRGVLYGTLGNGGIAYFTARSDLSTLFEDLALV
                     RPTELNFVPRIWDMLFQEFQSEVDRRSVDGADRAAVEAEVMADEAQNLLGGRFVAAMT
                     GSAPISAE"
BASE COUNT          851 a         1521 c         1626 g          903 t
ORIGIN      
        1 agggtggcca gtgcgttgaa ccggagaggg tgtgccgtaa cgatttcggc gcagcggtca
       61 ttgatcgccc gactgaggtc ggtgtgcccg gcggagacct gctgagtccc tggtgccgcg
      121 tcgtccgttg cccgaagccg ctgctcaccg acatccacca gtttcgcggt gagcgattcg
      181 ggtgtcgtcg gcttgggcca tacctcgttg atgagatcgg agggaagcac gtgttcttca
      241 acggcgatga gtgtcatggc gatcttttca tcggtgctgc ggcggctgag gctgcagtgt
      301 tcacggacaa gcgaggcagc agggtatggg cgttgccgtg catcacctgc gcgagcatgt
      361 cgtgccggtc tctgaagtag gcgtcgacgt tggtcgtgta ccagccggcc atctgggggc
      421 tgaccgcggg gaagtcggtg ccgaatagaa tctgttccgg tggcacgaag ttctccagtg
      481 ccaccaggtt cgtctcgaaa cccgacagcg ctgtttcgaa atagaaggtg cggaagtcgg
      541 caatgatctc ctcggggctg agctcgcagc ccacgtaatg ggagagcact gcgactctcg
      601 cggcgaggaa gggtgtgctc ccaccggaat gcgaaaggat gatccgcgtg ttcggatact
      661 gccgtttccg gccgcttgtg acgagatccg ctgcagcctt gtaggtttcg ttcggtacct
      721 cgctgacggg cgtgggaaga aaccgattcg gcatccggtt gcgtcccggt gtctgctcgc
      781 cgtgcaggaa caccagtgca tgccgccggt cgagttcggc ccagagcggc tcgaaaatgt
      841 cgtcgccgag cgatcgggcc tcctcgccgg tgccgtagac gtttgtcagg gtgatgccgt
      901 cggcgtcgag gtgatcaagg gcataggtga gctcgtccag ggcggcatcg acgtcagtgg
      961 gggttggcag gtgcgcgaag aagccgaacc ggccggggtg gtcgtcgacc gtctggcggg
     1021 cgatggtgtt gacctcgcgg gcgaatgccc gacgctcagg cgcgggcagc ccggactcca
     1081 ggtcgctcgg cagagacagc acggcggtct gaataccgag gccgtccatg aatgcgaggc
     1141 tggtttccgg tgtccagtgt gggctgccgc ccggaaagcg ccagccggag ttcgcaatta
     1201 ggccgcgctg tacgaactga gctagcttgc cgctcgggaa gaggtgatgg tgaagatcga
     1261 cccgtgcggg tcctgaactg ctcgaggtca tacctccagt gaagcgtcgg actgactcgt
     1321 ggcaattgtt gctagctgca aaatttggtg gccgcagcag gatgccattt agctacaggc
     1381 tgccgaccag ccggctgtcc ggtgacggac ccgacagtcc cctcaccaat cccttgccgg
     1441 tgatcagcgg caggtccaac gccgacagga gacctggttt ggcggcgcac accgccggaa
     1501 tcgcgttgac catccgggac gctccggcca tcctcgcgcc aagatcgtga tcgtggtcgt
     1561 ctgccatttc gaattcgcac cgcatggccg gtgaaccctc gatctcgaca cggtagacgc
     1621 cgcggccgga ggctccgccg taaccgagat cgcgtggctg gatcgatatg tgtggttgcg
     1681 gccagtccgg cgcgatgtcg tcgtacatgc ggctgacgtg gtcgatgacc agggtcggtt
     1741 tgccctcgac gtacccggtc aacgtggatc tcatcgcggc gatggttccg gcggcgatgt
     1801 gcccggtcgg ggtgtcgaac gagatgtccg cgggtatgcg ctccacggtt tccttgatgt
     1861 cgtcgacgcc aacctcgagg ccgtcggcca actggtgcag caccggcccc cagccgaacg
     1921 tgaacacacc cggtgtcgcg gcgaacggga cctcctctgg cggcttgccg aatccgagga
     1981 tgtcatacac ggcggacgcg tccgggtagg tcgcatagtt gaacatctcg gtgacacgga
     2041 tggactcgat ggtgcgggcg acccccgaca gtgccagcgg aagcacatca ttggcgaacc
     2101 cgctgtcgat gccggtggtg aaaaacgacc ctccacccgc aagtgcggcc tcgcgcaaag
     2161 ggtcggtgaa cgccggatcg accgcattgg gatacaccaa cggaacgacc gaacacgaca
     2221 ccacgttctt gccggcgcgc aggatcgaca ccatgtcgtt gaccgcctcc agcggccgca
     2281 ggttcgcggc ggcggcgtac accaccacat cggcatcacc gctgaccaag gcccccggat
     2341 cggtggtcgc gacgacgccg actgggtcga tgccgcacag ctcaccggca tctcttcccg
     2401 ccttcacgtc gctgtgcacg acgaggtcca ccaactccag ctcggggtga tcgatcacgc
     2461 cacgcaacgc gatcaccccc atcgaaccgg gtccccagac cgctactcga atagtcattg
     2521 ttcctccaaa tattaggaca ctaattgcta gtatttgggc agagcgtaag gagagaatcg
     2581 tgcccacgtc aacgcccaaa tcgccgccga cccggcgggc gatgaatgtc cttgacgcgt
     2641 tggccgcgtc atcgaatggc ttgacgtccg ctcagctgtc ccgtcagtgc ggcatcagca
     2701 cgtcgacgtg cgcgttggtg ttgggagaac tcgagtgtgg tgggtgggtc gcccgctacg
     2761 gcgatcgtcg ctatgggctg ggcagcggct tgttcgggtt ggttcgtggg ctgcgcaacc
     2821 aatttcccct gctcgaccag ggccgaaccg cactggtcga tctccacgag agcctggcgg
     2881 ccggctgttc gatgtcgcgc atcgacgacg accacctcac cgtcatcgac gcggtgggcc
     2941 acggcagcga tggtgagcac gttgtcggac agcgcttccc gatcgacccg ccgttcggcc
     3001 tggtggcgat ggcgtggcga gacgatgccg aagtagagcg ctggctgcga ggggtcacgc
     3061 cgcggctcgc ccaggccgac atcgaccagc atcggcaagt gctcgccgac gtccgcgcac
     3121 gcggctacgg agcgtggcga ttcgatgacg cccatgcgtc gctgcatgac cgggtcgccg
     3181 gcatcttggc gtcgctggaa ccgaccgcgt cggcggcacg gcagttgacc aagctgatga
     3241 caatcgtcac tctgcggtcg attacaagta ctctcgaaac agatttattg gcaacggaat
     3301 tcgtcgtcct gccgatcttc gggccgaccg gccaacccga ataccagatc gagatccaca
     3361 tcagccgtcc cgatgccctc acgctcgact cgctgaatac agcgctcgag aatgcgcaga
     3421 atgaactcgc gccgactggt acccacgctg gcgtccagaa agtttgacag cagtctccac
     3481 gaaggtgctc aactgagaac agggttcttc cctgccgacg ccattcaatc gcttaccgcc
     3541 agcccccatc tttgggtacc taccaacttc aaatgcccac tatgccggtg gcagagcgcc
     3601 ggggccgctc acagactgcc tggcgatatc ggcggtctgg cctaccgtgg agttgttgtt
     3661 acacaaacta attgagatgc tcagaaggac tagtgatctt atgtcgaccg atccccgcga
     3721 agagcggctg gcacgccgca tcaccggtct ttacgccacc gacccgcagt tcgccgacgc
     3781 ccggcccatc gaggcactca ccgcggcgat cgagcaaccc gcgctgcggc tgacggggat
     3841 cgtccgcacg gtcatgtcgg cctatgcgga acggcctgcg gtcggagagc gcgctgtcaa
     3901 cctcgccaca gatccggaga ccggccgcac ttcgctcgag cttttgcccc ggttcgagac
     3961 cgtcacgtac ggagcgttgt gggagcgcgt gaacgcggtc gtgcgcgcct tggccgacgg
     4021 cggcgtgcgg cccggcgatc gcgtctgtgt gctgggcttc acgagtgtgg actatgccac
     4081 gatcgacatg gcgttgggcc agttgggtgc ggtgtccgtt ccgctccaga ccagcgcacc
     4141 ggtcagtcaa ctgcggccca tcgtcgccga gacagaacct cgcgtattgg cttccagtgt
     4201 ctacgatctc ggcgacgccg tcgagctggt gctgacaggt cacgtgcctg gtcgtctggt
     4261 ggtgttcgac taccgccccg aggtggacga gcagcgcgag gcgttcgacg ccgccaggtc
     4321 gcggctcgcg gaggcgggaa gcccgttggt ggtggagact cttgccgagc tgctcgaacg
     4381 cggcgaatcg ctgcctgccg caccggagtt cgttcccgac gacgatgacg acgatccgtt
     4441 gaggttgctc atctacacct cgggcagcac cggcgccccg aagggtgcca tgtacccaga
     4501 acgattggtc gcgaacttct ggcgcaggtc gcgctggaat tggggcgcgc cgagtgtcga
     4561 gccgacgatc acgctcagct tcatgccgat gagccacgtg atgggtcggg gcgtcctcta
     4621 cggcacgctg gggaacggcg gaatcgccta tttcacggcc aggagcgatc tttcgacgct
     4681 gttcgaggac ctcgcgctgg tgcggcccac cgaactgaac ttcgtgccgc ggatctggga
     4741 catgctgttt caggagttcc agagcgaagt ggaccgtcgg tccgtcgacg gcgccgatcg
     4801 cgcggcagtc gaagccgagg tgatggcgga cgaggcacag aaccttctcg gtggacggtt
     4861 cgtcgcggcg atgacgggct ccgcgccgat ctcggccgag a
//
LOCUS       DASYLX010000556         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_27380, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000556 DASYLX010000000
VERSION     DASYLX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27380"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGP27_13400"
     CDS             complement(<1..292)
                     /locus_tag="VGP27_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV7852327.1"
                     /translation="MRVVVMGCGRVGASLADGLARIGHDVAVIDRDSTAFNRLSPEFP
                     GERVLGMGFDRDVLLRAGIEEASAFAAVSSGDNSNIISARVARETFGVERVVA"
     gene            391..2388
                     /locus_tag="VGP27_13405"
     CDS             391..2388
                     /locus_tag="VGP27_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7852328.1"
                     /translation="MSKLSTAARRLVLGRPFRSDRLSHTLLPKRIALPVFASDALSSV
                     AYAPEEIFLVLSVAGLSAYSLAPWIGVAVGAVMLIVIASYRQNVHAYPSGGGDYEVVT
                     TNLGPTAGLTVASALLVDYVLTVAVSMASAMSNIGSALPFINDHKVLIAVLAILLLAS
                     ANLRGLRESGTAFAIPTYAFMIGMYIMLGWGLFQIYVLGTPLRAESAGFELHSEHGDI
                     MGFAMVFLVARAFSSGCAALTGVEAISNGVPAFRKPKSRNAATTLLLLGGIAVSLFIG
                     IILLAKETGVKMAERPHEQLIGAPPDYHQKTMIAQLADAVFHDFPLGLYLIAGVTALI
                     LVLAANTAFNGFPVLASILAQDRYLPRQLHTRGDRLAFSNGILFLAFAAIAFIVAFQA
                     QVTALIQLYIVGVFVSFTLSQIGMVRHWTRLLTVENDPEVRRRMMRSRVINTIGFICT
                     GTVLIIVILTKFMAGAWIAILAMGGLFLIMKSIHKHYDTVARELEEQEAAEEGGIVLP
                     SRNHAIVLVSKLHLPTMRALAYARATRPDELEAITVSVDDAETRELVHKWEDSDISVP
                     LKVVASPYREITRPVLDYVKRISKESPRTVVTVFIPEYVVGHWWEQVLHNQSALRLKG
                     RLLFMPNVMVTSVPWQLNSSERLKRLQPHAAPGDARRGFLD"
     gene            2385..3581
                     /locus_tag="VGP27_13410"
     CDS             2385..3581
                     /locus_tag="VGP27_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEV7852329.1"
                     /translation="MTDDLTLTAGPPANGGSCVARHEGRVVFVRYALPGERVRARVVA
                     DHESYWHAEVVEVLEASPDRIDPLCPIAGVDGSGCCDLAFAAPEAARRLKGAVVANQL
                     SKLGGFHWCDEQDAFAEQVGGGEATGWRTRVRLDTGDDGRAGFHRYHSAELVTDLHCG
                     QLPAAALDGLSGNGWPAGAHLHVALDDDGVRHVVQSGPRVGKRTPTRVVEGGYEATQR
                     VSDRVWRVPVTAFWQAHRDAARVYSGLVAEWAQLSDGMVAWDLYGGAGIFAAVLGEAV
                     GARGQVVTVDTSRGASRSARTALADLTSVSVVTDSVRRALAAERRRADVAVLDPPRSG
                     AGREVIDLLAAAEVRRIVHIGCEAASFARDVGLYIKHGYRVEGLRAFDSFPLTHHVEC
                     VAVLTR"
     gene            3704..>4806
                     /locus_tag="VGP27_13415"
     CDS             3704..>4806
                     /locus_tag="VGP27_13415"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7852330.1"
                     /translation="MSRRRATAVAVALATPVAGFALLLSRPSIDGHWSHQPSHFWVVL
                     GAAVIAAALGWSIGTSAKRRADARLALVSMSFVASAAFLGLHALATPHVLISHANAGF
                     VVAVPVGLVIAAGFASWSAVPLEGGRARWVAAHLGAMRFALMATVVAWAVWSILELPP
                     LDNPMPVESGSPLMITLGIPAAVAFAVAAARYLVIARRRGAGLLVAVSAAWVLLGETA
                     LAVAITQSWRASWWLWHVLMMAAFAAIASASARMPSDERFSDLYLDDVAAGTRDVTVL
                     FADLKGFSRYSEDNPSDVVRAMLNTYFEAVLPGIRAAGGRVDRFIGDAVMVTFNVSAD
                     QPDHAVRAARAAVDFQAAARRVADQHPGWPRFRA"
BASE COUNT          693 a         1576 c         1649 g          888 t
ORIGIN      
        1 gggcgaccac ccgctcgacg ccgaaagtct cgcgggccac ccgcgccgag atgatgttcg
       61 aattgtcgcc ggacgacacg gccgcgaacg ccgaggcctc ttcgatgccc gcccgcagca
      121 ggacgtcgcg gtcgaatccc atccccagca cgcgttcgcc ggggaactcc gggctgagcc
      181 ggttgaaagc cgtgctgtca cggtcgatga cggctacatc gtggccgatg cgggcaagac
      241 cgtcggccag ggatgcgccc acccggccgc atcccatcac cactactcgc acctttgcgg
      301 tcctttcggc tgcagctcga aattgacgtc ttcaaatctg cgcactcgga acgctacagc
      361 caaaccagac tgggcttact cttggctctc gtgtccaagc tatcgaccgc cgcccggcgg
      421 ctggtcctcg gcaggccctt ccgcagcgac cggctttccc acacgctttt acccaagcgg
      481 atcgccctgc ccgtcttcgc ttccgacgcg ttgtcgtcgg tggcgtacgc gccggaggaa
      541 atctttttgg tgctgtcggt ggccggcctt tccgcgtatt cgttggcgcc atggatcggg
      601 gtcgccgtcg gagcagtgat gctgatcgtg atcgccagct accggcagaa cgtccacgcc
      661 tacccgtcgg gtggcggcga ctacgaagtc gtgaccacca acctcggccc gactgcaggg
      721 ctcacagtgg ccagcgcgct attggtggac tacgtgctca cggttgcggt gtcgatggca
      781 tcggcgatgt ccaacatcgg gtccgcgttg ccgttcatca acgaccacaa ggtgttgatc
      841 gccgtcctgg cgatcctgct cctcgcttcg gcgaacctgc ggggcctgcg cgagtctgga
      901 acggcgtttg cgatcccgac ctacgccttc atgatcggga tgtacatcat gctcggctgg
      961 ggcttgttcc agatctatgt actcggcact ccgcttcgcg ccgagtcggc gggcttcgag
     1021 ctgcactccg agcacggcga catcatgggc ttcgcgatgg tgttccttgt tgcgcgtgcg
     1081 ttctcgtccg gctgcgccgc actcacgggt gtggaggcta tcagtaacgg ggtcccggct
     1141 tttcgaaagc cgaagtcccg caacgccgca acgacattgc tgctgctcgg cggtatcgcg
     1201 gtcagcctgt tcatcgggat catcctgctg gccaaggaga ccggcgtgaa aatggccgag
     1261 cgaccgcacg agcagctgat cggtgcgccg cccgactacc accagaaaac gatgatcgcg
     1321 cagcttgccg acgcggtgtt ccacgacttt ccgctcgggc tctatttgat cgcaggcgtc
     1381 accgcgctca tcctggtcct cgcggccaac accgccttca acggcttccc cgtgctggcc
     1441 tcgattctcg cccaggaccg ctatctgccc cgtcagttgc atacccgcgg cgatcggctc
     1501 gccttctcca acggaatcct tttcctcgcg ttcgcggcga tcgcattcat cgtcgcgttc
     1561 caggcacagg tcaccgcgct gatccagctc tacatcgtcg gcgtcttcgt ctcgttcacg
     1621 ctcagccaga tcggcatggt gcggcactgg acacgattgc tgacggtcga gaacgatcct
     1681 gaggttcgcc gcaggatgat gcgctcccgg gtgatcaaca cgatcggctt catctgcacc
     1741 ggcactgtgc tgatcatcgt gatattgacg aaattcatgg cgggagcgtg gattgcgatc
     1801 ctggcgatgg gtggcctgtt cctgatcatg aagtcgatcc acaagcatta cgacaccgtc
     1861 gcgcgcgagc tggaagagca ggaggccgcc gaggagggcg ggatagtgct gcccagccgc
     1921 aaccacgcga tcgtgctggt gtccaagctt catctgccga cgatgcgcgc actggcgtac
     1981 gcgcgggcaa cccggccgga cgagttggag gcgatcaccg tcagcgtcga cgacgcggaa
     2041 acgcgtgagc tggtccacaa atgggaggac agcgacatct cggtgccgct caaggtggtc
     2101 gcctccccgt accgggaaat cacccgaccg gtgctggact atgtgaagcg gatcagcaag
     2161 gagtcgccgc gcaccgtggt gacggtattc atccctgagt acgtggtggg ccactggtgg
     2221 gaacaggtgc tgcacaacca aagtgctttg cgactgaagg gccggctgct gttcatgccg
     2281 aacgtgatgg tcacttcggt tccctggcag ctcaattcgt cggagcggct caagcggttg
     2341 caaccgcatg ccgcgcccgg tgatgcccgt cgaggcttcc tcgattgacc gacgatctca
     2401 cgctgaccgc aggcccgccg gccaacgggg gcagttgcgt cgcacgccac gaaggccgcg
     2461 tggttttcgt gcgctacgca ctgcctgggg agcgggtccg cgcgcgggtg gtcgccgatc
     2521 acgaatccta ctggcacgcc gaggttgtcg aggtgctcga ggcctcgccg gaccggatcg
     2581 acccgctgtg cccgatcgcg ggtgtggacg ggtcgggctg ctgtgatctg gcgttcgccg
     2641 ccccggaagc ggctcgtcgt ctcaagggtg ccgtcgtggc caaccagctg tcgaagctgg
     2701 gcgggttcca ctggtgcgac gaacaggatg cgttcgccga acaggtgggc ggcggtgagg
     2761 cgacgggctg gcggaccagg gtccggctgg acaccggtga cgacggccgc gccggttttc
     2821 accgctacca cagcgcggag ctggtcaccg acctgcactg cggacagctg cccgcggcgg
     2881 cgctcgacgg tctgagcggc aacggctggc cggcgggcgc gcacctgcac gtcgcgctgg
     2941 acgacgacgg agtgcggcat gtcgtgcaat ccggcccgcg tgtcggcaag cgcacgccga
     3001 cccgtgtcgt cgagggcggc tacgaggcga cgcagcgggt cagcgaccgg gtgtggcggg
     3061 tgccggtgac cgcgttctgg caggcccacc gcgatgcggc gcgggtgtac agcggtctgg
     3121 tggccgagtg ggcgcagctg agcgacggca tggtggcgtg ggatctgtac ggcggcgccg
     3181 gcatcttcgc cgccgtgctc ggcgaggccg tcggcgcccg tgggcaggtg gtgaccgtcg
     3241 acacgtcgcg gggagcgtcg cggtcggcgc ggaccgcact ggcggatctg acgagcgtct
     3301 cggtggtcac cgactcggtg cgccgcgcac tggctgccga gcgcaggcga gccgacgtcg
     3361 cggtgctgga cccgccgcgc tcgggtgcgg gccgcgaggt gatcgacctg ctggccgccg
     3421 ccgaggtgcg acggatcgtt cacatcggtt gtgaggccgc gtcgttcgcc cgagacgtcg
     3481 ggttgtacat caagcatggc tatcgggtgg agggtctgcg ggcgttcgac tcgttcccgc
     3541 tcacccacca cgtcgaatgt gtcgcggtgc tgacgcgcta ggtcggcgaa tcggcgaaga
     3601 tcccgtttcc ggggtgcgaa gtggcgatga tgaacgcgat gcgactcatc ctcactcgcg
     3661 ccgggcgccc acggtgtccc gcggattgcg cccgagcgtc ctgatgtcga ggcgtcgggc
     3721 gacagccgtt gcggtcgcgc tggcgacgcc ggtcgccggg tttgcgctgc tgctgtcccg
     3781 gccgtcgatc gacgggcatt ggagtcacca accgtcccat ttctgggtcg tgctgggcgc
     3841 ggccgtgatc gctgccgccc tcggctggtc gatcggcacc tcggccaagc gccgcgccga
     3901 cgctcgcctg gcgcttgttt ccatgtcgtt cgtagcgtcc gcggcatttc tgggtctgca
     3961 tgcgctggcc acgccccacg tcttgatctc gcacgccaat gcgggcttcg tcgtcgcggt
     4021 tccggtcgga ctggtgatcg cggccggatt cgcatcgtgg tccgccgttc cgctggaggg
     4081 cgggcgggcc cgatgggtcg ccgcccacct gggcgccatg cgattcgcac tgatggcgac
     4141 ggtggtggcg tgggcggtgt ggtcgatcct cgaacttccg ccgctggaca atccgatgcc
     4201 ggtcgaatcg ggctcgccgt tgatgatcac gctcggcatc cctgcggctg tggcattcgc
     4261 ggtggccgct gcgcggtacc tggtcatcgc acgccgacgc ggcgccggac tactggttgc
     4321 cgtttcggct gcgtgggtgt tgctcggtga gacggcgctg gcggttgcga tcacccagag
     4381 ctggcgcgcc tcctggtggc tgtggcacgt gctgatgatg gcggcattcg ccgcgatagc
     4441 gtcagcgtcg gcgcggatgc cttcagacga acgcttcagc gacctctacc tcgacgacgt
     4501 ggcggccgga acccgtgacg tcacggtgtt gttcgccgat ctcaagggct tcagcaggta
     4561 ctccgaggac aatccgtcag acgtcgtgcg cgcgatgctg aacacgtatt tcgaggccgt
     4621 cctgcccggc atacgggccg caggcggacg cgtggatcgg ttcatcgggg acgccgtgat
     4681 ggtgaccttc aacgtctccg ccgaccagcc cgaccacgcc gtcagggccg cgcgggccgc
     4741 cgtcgacttc caggccgccg ctcggcgcgt cgccgaccag catccgggct ggccgaggtt
     4801 ccgggc
//
LOCUS       DASYLX010000557         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3319156,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000557 DASYLX010000000
VERSION     DASYLX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319156"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VGP27_13420"
     CDS             <1..172
                     /locus_tag="VGP27_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852331.1"
                     /translation="VQDAVFWISFIAALTAAAAVIPADGRSPGLVVAGSALYLIGLAV
                     HAGVARRAQAPG"
     gene            183..962
                     /locus_tag="VGP27_13425"
     CDS             183..962
                     /locus_tag="VGP27_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HEV7852332.1"
                     /translation="MATAAPILDDLRAESDFLDGLVADLPADAWTTATPAPGWTVAHQ
                     IAHLLWTDRVALLSVNDEGGFNEVLGAAAEDPGGFVDRGAEEIAATAPADLLADWRRT
                     RHQVHDALRTVPDDRKLPWFGPPMSAASMATARLMETWAHGLDVADALDVKRPATARL
                     RSIAHIGVRTRDFAFAINGLAVPTDPFRVELHAPDGATTWTWGPQDAPQRVTGIAEDF
                     CLLVTQRRPLADLDVAAEGDDAQKWLTIAQAFAGPPGPGRN"
     gene            1007..1858
                     /locus_tag="VGP27_13430"
     CDS             1007..1858
                     /locus_tag="VGP27_13430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7852333.1"
                     /translation="MEIRLARPADAAECGRICYEAFAAIAARHGFPRDFPSAEIATAM
                     LSGLFAHPGFFGVVAEDDGRIVGSNVLDERSMIFGLGPITVDPDSQDRRVGLALMNAL
                     IERTAERGAPGLRLLQAAYHNRSMSLYAKLGFDVREPFATMQGDALGLQIAGHHVRVA
                     SEADVPACNALCSRVHGHDRGGEVRDAISQGAATVVERHGRITGYATGIAFFAHSVAE
                     SNDDLAALIGAADEFSGPGFLVPLRNTELVRWCLGHGLRIVHIMNLMTMGLYQEPRGA
                     YLASVAY"
     gene            complement(1876..2376)
                     /locus_tag="VGP27_13435"
     CDS             complement(1876..2376)
                     /locus_tag="VGP27_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEV7852334.1"
                     /translation="MDDCLSDGDGSATMWSAPLDTDVDGNGTLDAVGLDVDGDGGVDD
                     ALADLDGDGIADHAVRDGESYFTDDGSGTWAVSVDRGGLGPQLARGLRWFGLDGVEQV
                     GGPTADLDGDGQAGDRLIDIDGNGLADRALGDGIAYVDTDGDGAWDLKLTDSDGDGRA
                     DVANGL"
BASE COUNT          367 a          887 c          830 g          423 t
ORIGIN      
        1 cgtccaggat gcggtgttct ggatctcgtt catcgccgcg ctcacggcgg cggcggcggt
       61 catccccgcc gacggccggt cccccgggct cgtcgtcgcc ggttcggcgc tctacctcat
      121 cggcctcgcg gtgcatgccg gcgtcgctcg gcgcgcccaa gcaccgggct aaggtgaccg
      181 ccatggcgac agcggctccg attctggacg acctgcgagc cgagagcgac ttcctcgacg
      241 gtctggtcgc cgacctgccg gccgacgcct ggacgacggc gaccccggcg ccgggctgga
      301 ccgtcgcaca ccagatcgct catctgctgt ggaccgaccg ggtggccctg ttgtcggtca
      361 acgacgaggg cggtttcaac gaagtgctcg gtgcggcggc cgaagacccg ggcggtttcg
      421 tcgaccgcgg ggccgaggag atcgccgcca ccgcacccgc cgacctgctg gccgactggc
      481 gccgaacccg acaccaggtg cacgacgcgc tgcgcacggt gcccgacgac cgcaagctgc
      541 cctggttcgg accgccgatg agcgccgcgt cgatggccac ggcacgactg atggaaacgt
      601 gggcgcacgg ccttgacgtc gccgacgcgc tcgacgtgaa acggcccgcg accgcgcgcc
      661 tgcgctcgat cgcgcacatc ggtgtccgca cgcgtgactt cgccttcgcg atcaacggcc
      721 ttgcggtgcc gacggacccg ttccgggtgg aactgcacgc acccgacggc gcgacgacgt
      781 ggacgtgggg tccgcaggac gcaccgcagc gcgtcacggg tatcgctgaa gacttctgcc
      841 tgctggtcac tcagcgacgt cccctggcgg acctcgatgt cgcggccgag ggtgacgacg
      901 cgcagaagtg gctgacgatc gcgcaagcct tcgcaggccc accgggcccc gggcgcaact
      961 aggatctctt tgcgagaggc cgttggcgac gccgcaggag gtgcaggtgg aaatacgtct
     1021 agctcggccg gccgacgctg cggagtgcgg tcgcatttgt tatgaggcgt tcgccgcgat
     1081 agcggcccgg cacgggtttc cgcgcgactt tccttcggct gagatcgcga ccgccatgtt
     1141 gtcgggtctc ttcgcgcatc ccggcttttt tggcgtcgtc gccgaggacg acgggcgaat
     1201 cgtcggcagc aacgtgctcg atgaacgctc gatgatcttc ggtctcggtc cgatcactgt
     1261 cgacccggac tcacaggatc gcagagtcgg actcgcgttg atgaatgccc tgatcgaacg
     1321 gactgccgag cgcggtgctc cgggactgcg gctgttgcag gcggcctatc acaaccgctc
     1381 catgagcctt tacgccaagc tcgggttcga cgttcgtgaa ccgttcgcga cgatgcaggg
     1441 tgacgcatta gggctgcaga tcgctggtca ccacgttcgt gttgcatccg aggcggatgt
     1501 tccggcatgc aatgcgttgt gttcgcgcgt ccacggtcat gaccgcggcg gcgaagtccg
     1561 cgacgcaatc agccaaggcg ccgcaacggt cgtcgagcgc catgggcgaa tcaccggata
     1621 cgcgaccggc atcgcgttct tcgcccactc ggtcgccgag agcaatgacg acctggccgc
     1681 cctgatcgga gctgccgacg agttctccgg cccagggttt ctggttccgc tgcgcaacac
     1741 cgagcttgtg cgctggtgcc ttggccacgg cctccgaata gtgcacatca tgaacctgat
     1801 gacaatgggc ctgtatcagg agcctcgcgg agcgtacttg gcatcggtgg cttactgaac
     1861 gttcgcgtga caacgctaga ggccgttggc gacgtcggcc ctgccgtcgc cgtcgctgtc
     1921 ggtcagcttg aggtcccagg ctccgtcgcc gtcggtgtcg acgtaggcga tcccgtcgcc
     1981 gagcgcgcgg tcggccagcc cgttgccgtc gatgtcgatg agccggtcgc cggcctgccc
     2041 gtcgccgtcc agatcggccg ttgggccgcc gacctgttcg acgccgtcga ggccgaacca
     2101 ccgcagtccc ctagcaagct gtgggcccag cccgccgcgg tccacgctca ccgcccacgt
     2161 gcccgacccg tcgtcggtga aatagctctc gccgtcgcgc accgcgtgat cggcgatgcc
     2221 gtcaccgtcg agatcggcca gcgcgtcgtc cacgccgccg tcgccgtcga catccagacc
     2281 gaccgcgtcg agagtgccgt tgccgtccac atcggtatcc agcggtgcgc tccacatcgt
     2341 cgccgacccg tccccatcgg aaaggcagtc gtccataccc gctacgacgc ccggctagct
     2401 cctggcgttc caccacctgg tcaactcggc caccgcctcg tcgtggtcga gcggcccgcg
     2461 gtccagccgc agctccttga gatagcgcca cgcctcgccg acctgcg
//
LOCUS       DASYLX010000558         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3319219,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000558 DASYLX010000000
VERSION     DASYLX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319219"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGP27_13440"
     CDS             <1..330
                     /locus_tag="VGP27_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV7852335.1"
                     /translation="YVTDAGPLLPRLHKLVRADSTTRNKRRAARLQANYDDLEARIAE
                     LAAKEDLQRVRPDLDGNEIMRILDIPAGPQVGEAWRYLKELRLDRGPLDHDEAVAELT
                     RWWNARS"
     gene            349..828
                     /locus_tag="VGP27_13445"
     CDS             349..828
                     /locus_tag="VGP27_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEV7852336.1"
                     /translation="MDYCLSDGDGSATMWSAPLDTDVDGNGTLDAVGLDVDGDGGVDD
                     ALADLDGDGIADHAVLDGESYFTDDGSGAWAVSVDRGGQLRWFGLDGVEQVGGPTADL
                     DGDGQAGDRLIDMDGNGLADRALGDGIAYVDTDGDGGWDLKLTDSDGDGRADAANAL"
     gene            complement(825..1601)
                     /locus_tag="VGP27_13450"
     CDS             complement(825..1601)
                     /locus_tag="VGP27_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HEV7852337.1"
                     /translation="MATAAPILDDLRAESDYLDGLVADLPTDAWTTDTPAPGWTVAHQ
                     IAHLLWTDRVALLSVNDEGGFNEVLGAAAEDPSGFVDRGAEEIAATAPADLLADWRRT
                     RHQLHDALRTVPDDRKLPWFGPPMSAASMATARLMETWAHGLDVADALDVKRPATARL
                     RSIAHIGVRTRDFAFAINGLAVPTDPFRVELTAPDGSVWTWGPQEAPQRVMGWAEDFC
                     LLVTQRRPLAELDVTAEGDDAQKWLTIAQAFAGPPGPGRG"
     gene            complement(1612..>1783)
                     /locus_tag="VGP27_13455"
     CDS             complement(1612..>1783)
                     /locus_tag="VGP27_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852338.1"
                     /translation="VQDAVFWISFIAALTAAAAVIPADGRSPGLVVAGSALYLIGLAV
                     HAGVARRAQAPG"
BASE COUNT          278 a          607 c          667 g          231 t
ORIGIN      
        1 tatgtcaccg atgcgggccc cttgctgccg cggctgcaca agctggtgcg tgccgactca
       61 acgacgcgca acaagcggcg cgccgcgcgg ctgcaggcga actatgacga tctcgaggcg
      121 cggatcgccg agctggccgc caaggaagac ctgcagcggg tgcggccgga cctcgatggc
      181 aacgagatca tgaggatcct cgacatcccg gcaggcccgc aggtcggcga ggcgtggcgc
      241 tatctcaagg agctgcggct ggaccgcggg ccgctcgacc acgacgaggc ggtggccgag
      301 ttgaccaggt ggtggaacgc caggagctag ccgggcgtcg tagcgggtat ggactactgc
      361 ctttccgacg gggacgggtc ggcgacgatg tggagcgcac cgctggatac cgatgtggac
      421 ggcaacggca cactcgacgc ggtcggcctg gatgtcgacg gcgacggcgg cgtggacgac
      481 gcgctggccg atctcgacgg tgacggcatc gccgatcacg cggtgctgga cggcgagagc
      541 tatttcaccg acgacgggtc gggcgcgtgg gcggtgagcg tggaccgcgg cgggcaactg
      601 cggtggttcg gcctcgacgg cgtcgaacag gtcggcggcc caacggccga tctggacggc
      661 gacgggcagg ccggcgaccg gctcatcgac atggacggca acggcctggc cgaccgcgcg
      721 ctcggcgacg ggatcgccta cgtcgacacc gacggcgacg gagggtggga cctcaagctg
      781 accgacagcg acggcgacgg cagggcggac gccgccaacg cactctagcc gcgcccgggc
      841 ccgggcggtc cggcgaacgc ctgcgcgatc gtcagccact tctgcgcgtc gtcaccctcg
      901 gcagtgacat cgagctccgc caggggacgc cgctgagtga ccagcaggca gaagtcctcc
      961 gcccacccca tcacgcgttg cggcgcctcc tgcgggcccc acgtccacac ggagccgtcg
     1021 ggagccgtca gctcgacccg gaacgggtcc gtcggcaccg caaggccgtt gatcgcgaac
     1081 gcgaagtcac gcgtccggac accgatgtgc gcgatcgagc gcagccgcgc ggttgcgggc
     1141 cgtttcacgt cgagcgcgtc ggcgacgtcg aggccgtgcg cccacgtctc catcaaccgt
     1201 gcggtggcca tcgacgcggc gctcatcggc ggtccgaacc agggcagctt gcggtcgtcg
     1261 ggcaccgtgc gcagcgcgtc gtgcaactgg tgtcgggtgc ggcgccagtc ggccagcagg
     1321 tcggcgggtg cggtggcggc gatctcctcg gccccgcggt cgacgaaacc gctcgggtct
     1381 tcggccgccg caccgagcac ttcgttgaaa ccgccctcgt cgttgaccga caacagggcc
     1441 acccggtcgg tccacagcag atgagcgatc tggtgtgcga cggtccagcc cggcgccggg
     1501 gtgtccgtcg tccaggcgtc ggtcggcagg tcggcgacca gaccgtcgag gtagtcgctc
     1561 tcggctcgca ggtcgtccag aatcggagcc gctgtcgcca tggcggtcac cttagcccgg
     1621 tgcttgggcg cgccgagcga ctccggcatg caccgcgagg ccgatgaggt agagcgccga
     1681 accggcgacg acgagcccgg gggaccggcc gtcggcgggg atgaccgccg ccgccgccgt
     1741 gagcgcggcg atgaacgaga tccagaacac cgcatcctgg acg
//
LOCUS       DASYLX010000559         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3758476,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000559 DASYLX010000000
VERSION     DASYLX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758476"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..361
                     /locus_tag="VGP27_13460"
     CDS             74..361
                     /locus_tag="VGP27_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852339.1"
                     /translation="MTIPVPCYLVEWYHSAITEEPLDDTVARLKNSAVSMSAHGSPVR
                     LLNMLAVPTDEVLFAVFTADSASVVAETCDQAGIPAQRVTTATEVDLLRMQ"
     gene            complement(434..1285)
                     /gene="trpC"
                     /locus_tag="VGP27_13465"
     CDS             complement(434..1285)
                     /gene="trpC"
                     /locus_tag="VGP27_13465"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV7852340.1"
                     /translation="MSVLDGIIAGVLEDLEARKSKTPLAELRSRVADVPPALDPLPAF
                     REPGVSIIAEVKRKSPSKGELANIADPASLAGQYATGGASAISVLTERRRFGGSLADL
                     DAVRGHVAIPVLRKDFIVDPYQLWEARAHGADLALLMVVSLGDSQLADLIGLCDELGL
                     TALVESHTAEEIGRATDAGAELLGINARDLTTLKVDRSIFAALAPLVPEGTVRVAESG
                     VASPNDVADYHSSGADVVLVGEALVRSGDPRAAVRDFIETANANSVRPRPPTPDSAAA
                     SSTRHPW"
     gene            complement(2145..2609)
                     /locus_tag="VGP27_13470"
     CDS             complement(2145..2609)
                     /locus_tag="VGP27_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852341.1"
                     /translation="MASASKFEVRVDQPGNVLEVQFGPVEVDVPRSVGYYGGLAVAVG
                     MGLIEPPLALFIAAVPVYKALTNSRLPRLVRAIGEVLEGAAKPVGGDAEGVVQVEDQQ
                     KARTNAVEARDSGPATRSASSSGRRRAPARRSSAARAASGNSAKRSAAARAR"
     gene            complement(2901..3089)
                     /locus_tag="VGP27_13475"
     CDS             complement(2901..3089)
                     /locus_tag="VGP27_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852342.1"
                     /translation="MRNTNRIHDHYTVVSRAGDVNTSRVVIAGNSADAAETHREHYPG
                     RDIIRVFNKVMASVTLTA"
BASE COUNT          563 a         1017 c          951 g          583 t
ORIGIN      
        1 gacatgtcgc tgcgcgagat gtatagggaa acacccgatt ccttcgcgct ccatcttggt
       61 tagcgttcaa ttgatgacta ttccggtgcc ctgctacctc gtcgagtggt atcactccgc
      121 cattacggag gagccgctcg acgacacggt ggcgcgcctg aagaactccg cggtgtcgat
      181 gtcggcgcat gggtcaccgg ttcggctgct caatatgctc gcggtgccga ctgacgaggt
      241 gcttttcgcg gtcttcaccg cggactcggc gagcgtcgtc gcggagacat gcgaccaggc
      301 ggggatcccc gctcagcggg tcacgacagc gaccgaggtc gatctgctgc gaatgcagtg
      361 acgtcccggc cgcccggcct tcctgccagc ggtggctggc ggcatcaggt ctcgatctga
      421 ccgcggatca acatcaccac ggatgcctgg tggaagacgc ggccgcactg tccggcgtcg
      481 gcggccgagg ccggacagag ttcgcgttgg ccgtctcgat gaagtcgcgt acggcggcgc
      541 gtgggtctcc tgaacgcacg agtgcctcac cgacgaggac tacgtcggcg cccgagctgt
      601 gataatccgc gacatcgttc ggactcgcca cccccgattc cgccacgcgt accgtccctt
      661 cggggacgag cggcgcgagc gcggcgaata tcgaccggtc caccttcagg gtggtcaagt
      721 ctctcgcgtt gattcccaac aactccgccc cggcatcagt cgcgcggccg atttcctcgg
      781 cggtgtgcga ctcgacgagt gccgtcagcc ccagctcgtc gcacagtccg atcagatcgg
      841 ccaattggct gtcgccgagt gacacgacca tcagcagcgc gaggtcggca ccgtgggcgc
      901 gggcttccca caattggtag ggatcgacga tgaagtcctt gcgcagaacg ggaatggcaa
      961 cgtgaccacg tacggcgtcc aggtcggcca gggaccctcc gaagcggcgg cgttcggtca
     1021 gcacactgat cgcggaggcc ccgcccgttg cgtactggcc agccagggac gctggatccg
     1081 caatgttggc cagctcgccc ttactcgggc tcttgcgttt gacctccgcg atgatcgaca
     1141 cgccaggttc tcgaaatgcc ggcagtggat cgagcgctgg gggcacgtct gcaacccgcg
     1201 accgtagctc cgccagtggc gtcttgctct tccgagcttc caggtcctcc agaacgcccg
     1261 cgatgatccc gtcgagaacg ctcaagctgg tctcctcatc gtgtggtcgg caggcgccat
     1321 tttcatcgta acgatccctc gccggccccg ccacaacgga ctctatgaaa aaggtcaaat
     1381 gcgttgctgc gtagtcagtt tcgatattgg atgcgcgcga acccatggtg acttgcggcg
     1441 cctttggcgg ccgacgcggc gggcgctcaa gcgcagcagt gagggttact tgaagccgac
     1501 gaccatcgat tccggcctca caaaggtgct tgacgtaggt gtcgcggagc cccgcgtcgg
     1561 ccatccatca ttggtaatct gcgccggtgt agtcgatccg tcggggccca tgtcgatctg
     1621 tttaggctca ccgaaaggcc tgcagcgttg gcacgccctt cgtgtcgatc aacatctcgc
     1681 agccaaatca cggcaccgcc ctcggctagt gcgtcataag tcccttgcag cacagtctgt
     1741 ttggcattcg tttcccagcc atgcaggaca tgtccaaata cgcaggcctc ggccgacgga
     1801 agcgggttcg taaaggagtc gccggcacaa atcgagactc gagatccaag tccatgttga
     1861 gcgacgaatt tctcacaacc cgatggggaa gatcgaatcc ggtgccgatg aggtgtgggt
     1921 gccgcagtac cactgcgagg gcaccatttc ttgtgcagcg ccgaggtcac aaagcgtctg
     1981 gcgaccctcc cacgggaagg catccgtacg cgatcgcagg cgccggacgg taagcgctca
     2041 tggcggatag gaaagcggtc aaactccgag gatcggcgta ttacccagag ggaccaaatc
     2101 ggttcgaact ctgctgaggt ccatgcagcc gcaaaggtgt cgagctagcg cgcgcgggca
     2161 gccgccgacc gcttagccga attcccgctt gccgcgcgcg cagcacttga ccgccgggcg
     2221 ggcgccctcc gccggcccga tgaggatgcc gaacgcgttg ctggccctga gtcgcgagct
     2281 tcgacggcat tcgtccgtgc tttttgctga tcctcgactt ggacaacgcc ctccgcgtcg
     2341 ccgccgaccg gtttggccgc cccttccaaa acctcaccta tggcgcgcac caatcgagga
     2401 agccgcgaat tcgtcaaggc cttgtacacc ggcaccgcag cgatgaatag ggcaagcggc
     2461 ggctcgatca aacccatccc taccgcgacc gccagcccgc cgtaatatcc caccgaccgc
     2521 ggcacgtcca cctcgacggg gccgaactgc acttccaaga cgttgcccgg ttggtccacc
     2581 cgcacctcaa acttgctggc ggaagccatc gttcctcctt tggcaaaccc agacccttcc
     2641 ctgcggaacc ccggcgcgcc tcaacccgcc aagccaccgc gccaaccgga tacccggtct
     2701 cacgtccact tagctgacag gttcctgtca gtccctgcat gacccggtac atcaatttcc
     2761 gggtttgggg attgcacagc cacaggcccg ccaccaacag cagggtggcg ggccgggaat
     2821 cgcatctgtc ttgtgacgcc gcggacggac cggacctagc gaatggtctg ccgtgatatg
     2881 gagagaatgc agccagtttc ctacgcagtc agcgtcacag acgccattac tttgttgaag
     2941 acgcggatga tgtccctgcc ggggtagtgc tctcggtggg tctccgccgc gtcggcagag
     3001 ttgccggcga ttacgacgcg ggaggtgttg acgtcgccgg cgcggctgac aacggtgtag
     3061 tgatcgtgaa ttcgattcgt gtttctcatc gcatgtttcc ctttctaaga acga
//
LOCUS       DASYLX010000560         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3758545,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000560 DASYLX010000000
VERSION     DASYLX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758545"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VGP27_13480"
     CDS             complement(<1..703)
                     /locus_tag="VGP27_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEV7852343.1"
                     /translation="MIQVCSRCGTRWNVRDRQRVWCPRCNGTLLVPSAQPPSEWSARP
                     TAPAVRPNSQSERSVPPRLPQGYRWIAVRPGAAPPGRRRPRHLGPTPRYAAIPRWGLV
                     EHFDTAQVEQQAEPRRRASVGALRATLVTTIAVLGLAALVHIVRYALLIINRDVLLNP
                     VVAWLASWLGVVVSVAALFLLFATFVLLTNWLIARRSVAFEHVGREEHRPLWTLRAGC
                     LVPFVNLAWAPVFVIE"
     gene            complement(727..1071)
                     /locus_tag="VGP27_13485"
     CDS             complement(727..1071)
                     /locus_tag="VGP27_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7852344.1"
                     /translation="MESPAGIRQAEISDVPTTFDESVVLLDVRENDEWQRGHAAGARH
                     IPMGEVPARIGEIDPGAQLFVVCHLGGRSARVAQYLAQNGYAPINVSGGMQAWADAGR
                     PVVTDGGDAGSV"
BASE COUNT          298 a          561 c          533 g          295 t
ORIGIN      
        1 gttcgatgac gaagacgggt gcccacgcca ggttcacgaa cggcacaagg catccggcgc
       61 gcagcgtcca cagtggtcga tgttcctcgc gcccaacatg ttcgaaggcc accgaccgtc
      121 gagcgatcaa ccagttcgtc aaaagcacga aggtggcgaa caacaggaac agagcggcca
      181 cgctgaccac gactccgagc cagctggcca accacgccac caccgggttc aagagcacgt
      241 cgcggttgat gatcagcagc gcgtatcgca cgatgtgcac gagagcggcg aggccgagca
      301 ccgcgatcgt cgtgaccagg gtggcgcgca acgcgccgac cgaggccctg cgccggggtt
      361 cggcctgctg ctcgacttgt gcggtgtcga agtgttcgac aagcccccag cgtggaatcg
      421 ccgcatagcg cggcgtcgga cccaggtgtc gcggtcgcct gcggcccggc ggggcagcac
      481 cgggccggac cgctatccag cggtagccct gcggcaaccg tggcgggacg ctccgttcac
      541 tctggctgtt cggccgtacc gccggtgcgg tcggacgtgc gctccattcc gacggcggct
      601 gcgcggacgg caccagcaga gtgccgttgc accgcgggca ccagacgcgc tgccggtccc
      661 gcacattcca ccgcgtcccg cagcgggaac acacctggat catccgacca gcctagcgag
      721 cgttgctcag acgctgccgg cgtcgccgcc atcggtgacg accggccggc cggcgtcggc
      781 ccacgcctgc atgccgccgc tgacattgat cggtgcgtac ccgttttgag ccaggtattg
      841 ggccactcgc gcagagcggc caccgagatg gcacaccacg aacagttgcg cgccgggatc
      901 gatctcgccg atccgcgcgg gcacctcccc catcggaatg tgccgggccc cggcggcgtg
      961 gccgcgttgc cactcgtcgt tctcccgaac gtcgagcagg acgacagact catcgaacgt
     1021 cgtcggaacg tcggagatct cggcttgccg gatacctgct gggctctcca tgccctcatg
     1081 ctacgaggcg gctggtcatc gacacacgtc aagcagcaat gtgcggctat ccacagtttc
     1141 cacaggttta tgcacaggcc gccgtctgcg gaaggcgctt ctgaccttct gaatctgtgt
     1201 gattccttgg cgatcctggg gataacctga ggccgttatc gaacagtgat caacaggcag
     1261 gaaggccccg agcgaaatgg cttgccgctc taatctttgg gcgcacgcgc tttgatttct
     1321 ccatcgtcag ccgaagggct atgatcggcg acacaacgac aattgtgagg gatctcacgg
     1381 gggtgggtaa ttgaacgcgc aggtcagggg acattagatg acgacgtcgc atccgttcgg
     1441 acgagggtcc gccacgtctt ctgagtggca atcgtccagc cactcgtaca cccgcggcca
     1501 actgatggcc tgcgtccgcg ccggtttgat agcgctcgtt cttcttggcg tcctcgccct
     1561 catcgttttg ttctgacccg tttcgacacc aaacacagaa acggccctgg tctccgcagc
     1621 gggagagcca gggccgtttt tttgtctgcc tcaagcgggc gggtagacgc ccatgtcctt
     1681 ggccttc
//
LOCUS       DASYLX010000561         7706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3537471,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000561 DASYLX010000000
VERSION     DASYLX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3537471"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGP27_13490"
     CDS             complement(<1..402)
                     /locus_tag="VGP27_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7852345.1"
                     /translation="MAEILMAALSPVGHIGPLLTVAQALVDRGDRVTVLSGAGRADQI
                     RAVGATPATLPRKGDLDPALLESDSGREETSGIKRLNFDLVRLFVAPMPFQTKALADL
                     MAQKRFDAILADYGFFGVLPFALGDRSARPPL"
     gene            complement(602..1426)
                     /gene="fdhD"
                     /locus_tag="VGP27_13495"
     CDS             complement(602..1426)
                     /gene="fdhD"
                     /locus_tag="VGP27_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV7852346.1"
                     /translation="MGRVTARRRASHVTADEAVTRPETLVVEEPLEIRVNGKAITVTM
                     RTPGSDVELAQGFLLTEGVIGHRDDLVTVRYCRGAGADNLNTYNVLDATLSPGVEMPQ
                     LDISRNFYTTSSCGVCGKASLDAVRTISRYSPGDDASQVKAKTLSSMPEQLRAGQKVF
                     ASTGGLHGAALFDAEGTMLSVREDIGRHNAVDKVIGWAVEQNRVPLTGTVLLVSGRAS
                     FELTQKAVMAGIPVLAAVSAPSSLAVDLASQSGVTLVAFLRGESMNVYTRPDRIKR"
     gene            complement(1436..2530)
                     /locus_tag="VGP27_13500"
     CDS             complement(1436..2530)
                     /locus_tag="VGP27_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV7852347.1"
                     /translation="MRRLAASVALVLIVGGCSQQPPADNAAQPSAPAESVPTGLPLLP
                     SEVPTEGNRPAADNLPPPAEPQSAPPQTVKTPGRTVSVGDKPEGVVVDAVTRMVAVAK
                     RDPNELVLMNADTGDVVNRVPLPGVVRHLQLANPGGPVLVPVESANALIRVELPSGRA
                     LPQVVTGGMPHDASQAANGTVFVANEHGGTVTVLRGEEVVKVFADSVQPAGLAPVGES
                     MGLIDVRKNDLTVYDTQNLSIVGSVPAGDGPTHLVADKHGRMIAADTRGDAVRVFEPL
                     PTPHQVGEVAQPGGPYGITYDSTRDLLWVASSGTNEVVGYDMSQPTPREVSRFPTVQN
                     PYTLGVDANTGRLFIAGVTGGVVQVVDPQS"
     gene            2854..3996
                     /locus_tag="VGP27_13505"
     CDS             2854..3996
                     /locus_tag="VGP27_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7852348.1"
                     /translation="MSFSAGMASGNSYYAPPLRAAIVGTGGVSRVHGWRLQDLGSTVV
                     GVCGRDGAKARLLADEFGRYGHSRAGAFKDLRVMLDQTAPDVLHVCSPHGLHVAHAIE
                     AVKRGINVIVEKPMATASADALQLIAEAKAADVTAAVCLTKRSYPMVAEMRARILAGD
                     LGEVNAVSGGFWSWDSSHDDWAWGFDPAIAGTSYATADLGVHWLDLAEYVTDRRITEV
                     DARFSTLRPTRYRDGEPVHVEAEDYVRLFVEMTGGVIGSATFSGVTAGEPNGCAINVD
                     GTEGGFGWKVDRPNEVMHRRADGSAVVIVRNPDALSPGAARLSFSPAGHAEGFGDAFR
                     NLFRDVYCAIGGADVSYPTLADGAHMVALVEAIQASARARRAVSID"
     gene            4004..5101
                     /locus_tag="VGP27_13510"
     CDS             4004..5101
                     /locus_tag="VGP27_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7852349.1"
                     /translation="MDELLSAPAAETRERANAIELRGVSKSFDDAVALHPLDLDIRDG
                     EFFCLLGPSGCGKTTTLNIIGGFTAPSSGVVRIAGNDMTKRPPNRRPVNTVFQSYALF
                     PHLNVVENVSFGLRMARVHKREQRTRAQEALSLVGLGAFAERPVSQLSGGQAQRVAIA
                     RALVNKPSVLLLDEPLGALDLKLRKRLQTELSLIQRHVGTTFVFVTHDQEEAMALADR
                     IVILNEGRIEQIGTPEEIYRRPASLFAADFIGESNIFHGNKKGDTFVLKNGARVPISG
                     HAPDTCSSLVVRPEALRIGKDGFIGAQVLSRGYLGSITRMVLQLENSELQVVSSLNDG
                     DIENLAEIGLESGDFVKLSWSTEAAHPLVAH"
     gene            5117..6244
                     /locus_tag="VGP27_13515"
     CDS             5117..6244
                     /locus_tag="VGP27_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7852350.1"
                     /translation="MSRITEVKLSRRSFVGGALMLGAASPMLLSACSSASDSSGGGSG
                     GGGNVNWLTWSDHYSQDQLAKVADTTKITGRPKLFSDNADALLQVKQTGSQFDMVSAD
                     ALWVKKYMEEGLIEPFDLASISSSSQLYPMATKFEFLNDPKGALGYPFSWSTVQIYFN
                     PDKVKTPPTSWESLTDPIYKGMIVAENIPTDLMAIAGIATGAKGSPYAQTPEQISSSS
                     NFLNALKPNVVKLASQNNEIVTMMASGEAAIGISNLGTDVRVKDAGGPELKSAYPPEG
                     TIGFIDSEMIVAKGANKALVKPFMDAMQQSEWIVANFTTNWRPLFNQKAAEALTADPK
                     TADNAKRLFMTNPEKALQMTLKGPAQDQQAYTEAFNKVFGA"
     gene            6246..7172
                     /locus_tag="VGP27_13520"
     CDS             6246..7172
                     /locus_tag="VGP27_13520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852351.1"
                     /translation="MTVASYARDAGGGSGDTEPRRADVKKERRDWRQFVGFVPAAILF
                     GVFFIAPLCLIVVYSFWLTKNYELVPDFTSANYKTILGTGTYVRTFGKTVLMAALATL
                     TALALAFPFCYWLVRYVPKKLQRVGLLLVILPFWTSYLLRVYAWVAILGDNGAINSML
                     KALGLTDGPIRLFLYDNPGVFIVLVYLYFPFAALTLYASLERFDWRLLHAAVDLGATP
                     MRAIANTLLPQMRLALVTAGIFVFIPILGEYLAPSAIGGTHGVMMGNLVANFFSGYLL
                     PLGAAASVLIALVITIVLIVFRRSLDTKEIYV"
     gene            7165..>7706
                     /locus_tag="VGP27_13525"
     CDS             7165..>7706
                     /locus_tag="VGP27_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852352.1"
                     /translation="MSDDLAAVNPRARLFRIILSFYGVGIYIFLFAPIAVMILLSFNT
                     SRVVGFPMAGFTTDWYSRLTHDTVILNALEKSLIVAFWVTVLSTVIGTMAAFPLVRAN
                     IKNRGGVRVFITLPIMMPGLLIGVAILVLVTNVLHIQPSLYTAIIGQTVLATPFVVLV
                     VSSRLEALDRNLERAAADLGA"
BASE COUNT         1322 a         2427 c         2488 g         1469 t
ORIGIN      
        1 taatggcggc cgagcagacc ggtccccgag cgcgaacggc aggacgccga agaagccgta
       61 gtcggcgagg atcgcgtcga accgtttctg cgccatcagg tcggcaagcg ccttcgtctg
      121 aaaaggcatc ggcgccacga agagccggac gaggtcgaag ttgagccgct taatgccgct
      181 ggtttcctct cggccgctgt cggattcgag cagcgccggg tcgaggtcgc ctttccgcgg
      241 cagcgttgcg ggtgtggcgc ctaccgctcg tatctgatcg gctcgaccgg cgcccgacaa
      301 cacggtgacg cgatctccac ggtccaccag ggcctgtgcg acggtcagca gtgggccgat
      361 gtgcccgacg ggtgacagcg ccgccatcaa gatctccgcc atcgtttgtg ttcccttcga
      421 tcgttgttcg acgggattga tcgtcggcga tcgcgggggc gcgagcttgg aggttgtatg
      481 gagatttggg ggagatcgca ggtagaggca ctgcgcgact cgcgagcacg catcgtcgta
      541 cggttttagc cgcctgatcc gtacctaagt gagcgctcgc ccgcgccgag gctctgcgcc
      601 cctatcgctt gatgcggtcc gggcgggtgt agacgttcat cgactcgccg cgcaggaacg
      661 ccaccagcgt cactccggac tggctggcca gatcaaccgc gagtgacgac ggcgccgaga
      721 ccgccgcgag cacgggaatg cccgccatca cggctttctg cgtgagctcg aacgaggcgc
      781 gcccgctgac cagcagcaca gtgccggtca acgggaccct gttctgttcg acggcccagc
      841 cgatgacctt gtcgacggcg ttgtgccgac cgatgtcctc ccgcacggac aacatggttc
      901 cctcggcgtc gaagagcgcc gcgccgtgca ggccgccggt gctggcgaac accttctgcc
      961 cggcacgcaa ctgctccggc atcgacgaca gcgtcttcgc cttgacctgt gaggcgtcat
     1021 cgccggggga gtagcggctg atcgtgcgga ccgcgtcgag ggacgccttg ccgcacaccc
     1081 cgcacgatga cgtcgtatag aagttgcggg agatgtccag ctgcggcatc tccacgccgg
     1141 gagagagcgt cgcatccagc acgttgtagg tgttcaaatt gtcggcgcct gcgcccctgc
     1201 agtagcgcac ggtgaccagg tcgtcgcgat gcccgatgac tccctcggtc agcagaaagc
     1261 cctgcgccag ttcgacatcg gagcccggcg tccgcatcgt gaccgtgatc gccttgccgt
     1321 tgacgcggat ctccagcggc tcctcgacga ccagtgtctc cggtcgcgtc accgcctcgt
     1381 cggcggtgac gtggctggcg cgacgccgtg cggtgacccg tcccataaaa ccaatctacg
     1441 actgcgggtc cacgacctgc accactccgc cggttacgcc cgcgatgaac agcctgccgg
     1501 tgtttgcgtc gacgcccagc gtgtaggggt tctggacggt cggaaagcga ctcacctcgc
     1561 gcggtgtcgg ctggctcatg tcgtagccga ccacctcgtt ggtgcccgat gaagcgaccc
     1621 acagcaggtc gcgcgtcgag tcgtaggtga ttccgtacgg accgcccggc tgtgcgacct
     1681 cgccgacctg gtgcggcgtt ggcagcggct cgaagacgcg cactgcgtcg cctctggtgt
     1741 cggcggcgat catgcggccg tgcttgtcgg ccaccaggtg agtgggtccg tcgccggccg
     1801 gcaccgaacc gacgatggac aggttctgag tgtcgtagac ggtcaagtcg ttcttgcgga
     1861 cgtcgatgag gcccatcgat tcgccgaccg gcgccaggcc cgcgggctgc acgctgtcgg
     1921 cgaaaacctt cacgacctct tcaccgcgca gcacggtgac ggttccacca tgttcgttgg
     1981 cgacgaacac ggtaccgttc gcggcctgcg acgcgtcgtg aggcatccct ccggtgacga
     2041 cctgcggcag cgcccgtccg ctcggcagtt cgacgcggat cagcgcgttg gcgctctcga
     2101 cggggacgag tacgggtccg cccgggttgg ccaattgcag gtgccggacg actccgggca
     2161 aggggacccg gttgacgacg tcgccggtgt cggcgttcat gaggacgagt tcgttgggat
     2221 cgcgtttggc gacggcgacc atgcgggtga ccgcgtcgac cacgacgccc tcgggcttgt
     2281 ctccgaccga cacggtgcgt ccgggggtct taaccgtctg cggcggtgcc gattgcggct
     2341 cagcgggggg cggaagattg tcggcggccg gccggttgcc ctcggtcggc acctcgctcg
     2401 gcagcagggg taggccggtc ggcaccgatt cggccggcgc cgacggctgc gcagcgttgt
     2461 ctgccggcgg ttgctgcgag caacctccga cgatcaggac cagcgctacc gatgccgcga
     2521 gccgccgcat gtttccaaag tacttgcccg cgctgaaaga ccgagtcata ttcagactgc
     2581 cgagcctaga cggtgccgat ttcgcctgga agcggttcgc tgctaagccg cttcgctaac
     2641 gcggcggcgg cgattgctgt gcatttggac cgcggtcgtt caccctttgt tcctcaatgt
     2701 gtcgtgatgt attgacaatc ggtcaacgca tgtccagact tcagtcacac ggcacgccgg
     2761 agccactgaa tgccccacgt ctggcgcatc gaacggtgcg gtaacacccg atggagaaga
     2821 cacttggcag tcgctacagc aaacaatccc tcgatgagtt tcagcgcggg gatggctagc
     2881 ggcaacagct actacgcgcc acccctgcgt gctgcgattg tcggcaccgg tggggtttca
     2941 cgcgtccacg gctggcggct acaggatctc ggctcgacgg tcgtgggcgt ctgtgggcgc
     3001 gacggagcca aggcccgctt gcttgccgac gagttcggtc ggtatggcca ctcgcgggcc
     3061 ggcgctttca aagatcttcg ggtcatgctc gaccagaccg cgcccgacgt gctgcatgtc
     3121 tgcagcccgc acggacttca cgtcgcgcat gccatcgaag cggtcaagcg cggaatcaat
     3181 gtgatcgtcg agaagccgat ggcgaccgct tcggccgatg cgcttcagct gatcgcggag
     3241 gcgaaggccg cagatgtcac ggcggcggtg tgcctgacca agcggtcata tccgatggtc
     3301 gccgaaatgc gggcgagaat cctggcgggc gacctcggcg aggtgaacgc cgtcagcggc
     3361 ggattctggt cgtgggattc gagtcacgac gactgggcgt gggggttcga tccagcaatc
     3421 gccggaacat cttatgccac agcggatctg ggtgtgcatt ggctggatct ggccgagtat
     3481 gtgaccgacc ggcggatcac cgaggtcgat gcgcggttct ccacgctgcg gcccacccgg
     3541 taccgcgatg gtgagccggt ccacgtcgag gccgaggatt atgtgcggct tttcgtcgaa
     3601 atgaccggtg gcgtaatcgg atccgcgacg ttctccggcg tcaccgcagg cgagccgaac
     3661 ggctgcgcta tcaacgtcga tggcaccgag ggcggcttcg gttggaaggt cgaccgtccg
     3721 aacgaggtca tgcaccgccg ggccgacgga tcggcggtcg tgatcgtccg caaccccgac
     3781 gcgctgagtc ccggcgctgc gcggttgtcg ttctcgccgg ccggtcacgc cgagggcttc
     3841 ggcgacgcgt tccgaaatct gttccgcgac gtctactgcg cgatcggcgg cgccgacgtc
     3901 tcctatccga cgctggccga cggcgcccac atggttgccc tcgtcgaagc aatccaggcg
     3961 agcgcacgtg ctcgaagggc ggtaagcatt gactagcttc ccggtggacg aactcctctc
     4021 ggcacccgcg gccgagacgc gcgagcgggc caacgcaatc gaactgcgcg gcgtgagtaa
     4081 gagtttcgac gacgcggtgg ctttgcatcc tcttgatctc gatatccggg atggcgagtt
     4141 cttctgcctg ctcgggccgt cagggtgcgg caagacgacg acgctgaaca tcatcggagg
     4201 attcaccgcg ccgtcctcgg gtgtggtgcg cattgccgga aacgacatga ccaaacggcc
     4261 gcccaaccgg cgaccggtca acaccgtctt tcagtcctac gcgttgttcc cgcacctcaa
     4321 cgtggtggaa aacgtctcct tcggattgag gatggctcgc gtgcataagc gcgaacagcg
     4381 gactcgcgca caggaagcgc tcagcctggt cggcctcggg gcgttcgccg aacgtccggt
     4441 cagccagctg tcgggagggc aggcacaacg cgtcgcgatc gctcgagcgc tcgtcaacaa
     4501 gcccagtgtg ctgctgctcg acgaacctct cggtgcgctt gacttgaagc tgcgtaagcg
     4561 gctgcagacc gagctttcgt tgatccagcg tcatgtcggt acgacgttcg ttttcgtcac
     4621 ccacgaccag gaagaagcga tggccctcgc ggaccgcatc gtcatcttga acgaaggacg
     4681 tatcgagcag atcggaacgc cggaggagat ctaccgacgg ccggccagcc tgttcgcagc
     4741 cgacttcatc ggggaatcga atatttttca cggcaacaag aagggggaca ccttcgtact
     4801 gaaaaacggt gcgagggttc cgatctcggg acacgcgccg gatacctgca gctcgctggt
     4861 ggtccggccg gaagccctgc ggatcggcaa ggacggtttc atcggcgcac aagtgctctc
     4921 gcgtggttat ctcgggtcga ttactcgcat ggtgcttcag ctggagaatt ccgaattgca
     4981 ggtcgtgtcg tcgttgaacg acggcgacat tgagaatctc gctgaaatcg gcctcgaatc
     5041 aggtgacttc gtgaagctgt cctggtcgac ggaagcagcc catccgctgg tagcccactg
     5101 agagtcagga agtcgcatgt cccgaatcac agaagtaaaa ctgagccgcc gctcgttcgt
     5161 cggcggagcg ttgatgctcg gcgcggcaag cccgatgctg ttgtcggcat gctcgtcggc
     5221 gagtgactcg tcaggcggcg gatccggcgg cggtggaaac gtcaattggc tgacgtggtc
     5281 ggatcattac tcgcaggacc agctcgccaa ggtcgccgat acgacaaaga tcaccggacg
     5341 gccgaagctg ttctccgaca acgctgatgc cttgcttcag gtgaagcaga ccgggtcaca
     5401 gttcgacatg gtctcggccg acgcgctgtg ggtgaagaag tacatggaag aaggcttgat
     5461 cgagccgttc gacctggcgt cgatatcctc gagttctcag ctgtacccga tggccaccaa
     5521 gttcgagttc ctcaacgatc ccaagggcgc gttgggatat ccgttctcgt ggtcgaccgt
     5581 gcagatctac ttcaaccccg acaaggtcaa gactccgccg acgtcgtggg agtcgctgac
     5641 cgacccgata tacaagggca tgatcgtcgc ggagaacatt ccgaccgacc tgatggcgat
     5701 cgcagggatc gccaccggtg ccaaagggtc accctatgca cagacgcccg agcagatctc
     5761 gtcgtcgtcc aacttcctga acgccctgaa gccgaacgtg gtgaagctcg cctcgcagaa
     5821 caacgagatc gtcaccatga tggccagcgg ggaagccgcg atcggcatct cgaacctcgg
     5881 caccgatgtg cgtgtcaagg atgcaggcgg cccggaactc aagtccgcgt acccacccga
     5941 gggcacgatc ggtttcatcg actcggagat gatcgtcgcg aagggcgcga acaaggctct
     6001 ggtcaagccg ttcatggatg cgatgcagca atcggagtgg atcgtcgcga acttcaccac
     6061 gaactggcgt ccactgttca accagaaggc cgccgaggcc ctgaccgccg atccgaagac
     6121 ggccgacaac gccaaacggc ttttcatgac caatccggag aaggcgttgc agatgaccct
     6181 caaggggccg gcccaggatc agcaggccta caccgaggcg ttcaacaagg tcttcggggc
     6241 gtgagatgac cgtagcctcc tatgcccgcg atgcgggcgg ggggtcgggt gacacggaac
     6301 cacgacgcgc tgacgtcaag aaggaacgcc gagattggcg tcagttcgtc gggttcgtac
     6361 cggccgcaat ccttttcggg gtgttcttca tcgctccgtt gtgcctgatc gtcgtctact
     6421 cgttctggct caccaagaac tacgagttgg tgcccgactt cacctcggcc aactacaaga
     6481 cgatcctggg tacgggcacg tacgtgcgga cattcggcaa gaccgtgctg atggccgccc
     6541 tggccacgct cactgcgctg gcactggcct ttccgttctg ctattggctg gtgcgctatg
     6601 tcccgaagaa gctgcagcgg gtcgggctgc tgctggtgat cctgccgttc tggacgagtt
     6661 atctgctgcg ggtgtacgcg tgggtggcga tcctcggcga caacggcgcg atcaattcga
     6721 tgctcaaagc cctcggtctg accgacggcc cgatccggct gttcctgtac gacaatcccg
     6781 gcgtgttcat cgtcttggtc tatctgtact ttccgttcgc ggcgctcacg ctctatgcat
     6841 ctctggagag attcgactgg cggcttctgc atgcggcggt cgacctcggc gccacaccga
     6901 tgcgcgccat cgccaacact ctccttccgc aaatgcggct tgcgttggtg acggccggaa
     6961 tcttcgtatt catcccgatt ctcggcgagt acctcgcacc gtcggcgatc ggcggtacgc
     7021 acggcgtgat gatgggcaac ctggtggcca acttcttctc cggctacctg ctcccgctcg
     7081 gcgcagcggc cagcgtgttg atcgcgttgg tgatcaccat cgtgttgatc gtcttccggc
     7141 gcagtctgga taccaaggaa atctatgtct gacgatcttg cggcggtgaa tccgcgggcc
     7201 cggctgttca ggatcatcct gtcgttctac ggcgtcggca tttacatctt cctgtttgcc
     7261 ccgatcgccg tgatgatcct gctgtccttc aacacgtcca gggttgtcgg ctttccgatg
     7321 gcggggttca ccaccgactg gtattcgcgg cttacgcacg acaccgtgat tctcaatgcg
     7381 ctcgagaagt cgttgatcgt ggcgttctgg gtcaccgtgc tctctaccgt catcggcacc
     7441 atggcagcct tcccgctggt gcgcgccaac atcaagaacc gcggcggggt acgggtcttc
     7501 atcaccctgc cgatcatgat gcccggtttg ctcatcggcg tcgccatcct ggtgctggtg
     7561 accaatgtgc tgcacatcca gccatcgctt tacaccgcca tcatcggaca gacggtcctt
     7621 gccacaccgt tcgtcgtcct ggttgtgtca tcacggttgg aggcgttgga ccgcaacctc
     7681 gagcgcgccg ccgccgatct cggcgc
//
LOCUS       DASYLX010000562         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2769323,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000562 DASYLX010000000
VERSION     DASYLX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769323"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGP27_13530"
     CDS             <1..188
                     /locus_tag="VGP27_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006865832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV7852353.1"
                     /translation="LVLFVYGLVIDHASAANFVPVNSADNWLHLVLAIGMIGLGVALS
                     RANSGARSPVNRTSPDA"
     gene            287..751
                     /locus_tag="VGP27_13535"
     CDS             287..751
                     /locus_tag="VGP27_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV7852354.1"
                     /translation="MAKNPKLMAVQGAAVIVAAVFLIVGVLGFLPGVTSRYDQLQLIG
                     HHSGAMLFGVFAVSGLHNAVHMAFGVAGLAMARTYALARAYFLLGGLLYLGLWVYGLL
                     IDHGSSANFVPVNNADNWLHFGLGLGMVLLGLTLAGQHDPTKRPKRRAKVRT"
     gene            complement(748..1686)
                     /locus_tag="VGP27_13540"
     CDS             complement(748..1686)
                     /locus_tag="VGP27_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEV7852355.1"
                     /translation="MSTVAAIDCGTNSIRLLIADSVDGSLRDLHREMRIVRLGQGVDA
                     TGQFAPEAIERTKAALADYAELLKRHDVSRVRMVATSATRDAANRDVFFAMTADVLGA
                     AVPGAVAEVISGDEEARLSFNGAVHELDGAAAPYVVVDLGGGSTEVVLGEREAQAGYS
                     ADIGCVRLTERCLHTDPPTAVEVAAAREVVRERLGEALRVVPVERARTWVGVAGTMTT
                     LAALAQRMTTYDPAAIHLSRVGFGALLPVCEEIIAMSHDQRAALGPMHEGRVDVIGGG
                     AIIVEELADALGSRAGIDELVVSEHDILDGIALSIV"
     gene            complement(1715..2206)
                     /locus_tag="VGP27_13545"
     CDS             complement(1715..2206)
                     /locus_tag="VGP27_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEV7852356.1"
                     /translation="MVERPDLDAVARQLGREPRGVLEIAYRCPNGEPGVVKTAPRLPD
                     GTPFPTLYYLTHPALTAAGSRLESSGLMREMTDRLHDDPQLAAAYRRAHESYLAERDA
                     IESLGTTFSGGGMPERVKCLHVLIAHSLAKGPGVNPFGDEALAMLAVEPGMAGILDRE
                     VWT"
     gene            complement(2199..2888)
                     /locus_tag="VGP27_13550"
     CDS             complement(2199..2888)
                     /locus_tag="VGP27_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV7852357.1"
                     /translation="MPEAKRPDPKRRSPASRPAKTAKSGKAGDTARGRPRAGSPARRE
                     PRKAESRPKPQPDVEPTTADSIRRSIAEAAEQQSEQRLGSTARRAAILAAVVCVLTLT
                     IAGPVRTYFSQRTEMKQLAASEEKLRVEIAGLEEQKAKLADPVYIAAQARERLGFVKP
                     GDTPYQVQLPPGAVAPGEPAREAAAARAGQPWYTSLWHTIADDPHNIPTTVPSVPPAP
                     PAPPDAPPPGG"
     gene            complement(2892..4214)
                     /gene="eno"
                     /locus_tag="VGP27_13555"
     CDS             complement(2892..4214)
                     /gene="eno"
                     /locus_tag="VGP27_13555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV7852358.1"
                     /translation="MPIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTG
                     EHEAVELRDGGSRYGGKGVRKAVEAVLDEIAPTVIGLSADDQRVIDQALLDLDGTPDK
                     SRLGANAILGVSLAVAKAAAESAGLPLFRYVGGPNAHVLPVPMMNILNGGAHADTGVD
                     VQEFMVAPIGAPSFKESLRWGTEVYHSLKSVLKKQGLATGLGDEGGFAPDVADTKAAL
                     DLILSAINATGFKPGTDVALALDVAATEFYTEGTGYSFEKQTRSADQMIEFYAGLLDA
                     YPLVSIEDPLSEDDWDGWVALTTTIGDRVQIVGDDLFVTNPERLEEGIERGAANALLV
                     KVNQIGTLTETLDAVSLAHNSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPAR
                     SERVAKYNQLLRIEETLGDAARFAGDLAFPRFAPGDEPLVRSVDGEAK"
     gene            complement(4381..>4672)
                     /locus_tag="VGP27_13560"
     CDS             complement(4381..>4672)
                     /locus_tag="VGP27_13560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEV7852359.1"
                     /translation="SGLLDGDPGIDRAMGPMQFIPETWRLYGVDANNDGKVSPDNFDD
                     AALSAAGYLCWRGKNLSTPRGWMNALRAYNLSDQYARTVRDWATAYADGHPL"
BASE COUNT          696 a         1614 c         1553 g          809 t
ORIGIN      
        1 acctggtgct gttcgtgtac ggcctggtga tcgatcacgc cagcgctgcc aatttcgtgc
       61 ccgtgaacag cgccgacaac tggcttcatc tggtgttggc gatcgggatg atcgggctgg
      121 gtgtcgcgct gtcgcgggcc aactcgggcg cacggtcacc cgtgaacagg acatcacctg
      181 acgcctgatc gccggcggcc acacctagcc tcgattagcg cgccagggcc gcgggtactt
      241 catgcctgcg ggcccgtcaa taaccgggct gaagggagac gaggtgatgg cgaagaatcc
      301 caaactgatg gccgttcagg gcgcggcggt gatcgtcgcc gccgtgttcc tgatcgtcgg
      361 agtgctgggc ttcctacccg gtgtcacctc aagatacgac cagctgcagt tgatcgggca
      421 tcactccggc gcgatgctgt tcggcgtctt cgcggtgtcc ggcctgcaca acgccgtaca
      481 catggcgttc ggcgttgccg gcctggcgat ggcacggacc tatgccttgg cacgcgccta
      541 ctttctgctc ggcggccttc tctacctggg cctttgggtg tacgggttgc tcatcgacca
      601 cggcagctcg gccaacttcg ttccggtgaa caacgccgac aactggctgc acttcggcct
      661 cgggctcggc atggtgctgt tgggcctcac cctggcaggc cagcacgacc cgaccaaacg
      721 tccgaagcgg cgcgcgaagg tgcgcaccta gacgatcgac agcgcgatgc cgtcgaggat
      781 gtcgtgctcg ctgaccacca gctcgtcgat tccggcccgg ctgcccagcg catccgccaa
      841 ttcctccacg atgattgcgc caccgccgat tacgtcgacg cggccctcgt gcatcgggcc
      901 caacgcggcg cgctggtcgt gactcatcgc gatgatctcc tcacacaccg gaagcagtgc
      961 gccgaacccg acccgcgaca gatgaatcgc ggcagggtca tacgtcgtca tccgttgcgc
     1021 aagcgccgca agggtggtca tggtccccgc gacgcccacc cacgtgcgtg cccgctcgac
     1081 gggcaccacc cgcagcgcct cccccagccg ttcgcgcacc acttcacgcg cggccgccac
     1141 ctcgaccgcc gtgggtggat cggtgtgcag gcagcgttcg gtcagccgga cgcagccgat
     1201 gtcggcggag tagccggctt gcgcctcccg ctcccccagc accacctcgg tggatccgcc
     1261 gccgagatcg acgacgacgt aaggcgctgc agcgccgtct agttcgtgga cagcgccgtt
     1321 gaaggacagc ctggcttcct cgtcgccgct gatcacctct gcaacagcgc cgggtacggc
     1381 ggcgccgagc acatcggctg tcatggcgaa gaacacgtca cggttggcgg cgtcacgggt
     1441 cgccgacgtc gcgaccatcc ggactctcga aacatcatgg cgcttcagca gttcggcgta
     1501 atcggcgagg gcggccttcg tccgctcgat cgcctcaggc gcgaactgcc cggtggcgtc
     1561 cacgccctgg cccagtcgca cgatgcgcat ctcgcggtgg agatcccgaa gcgaaccgtc
     1621 gaccgagtcg gcgatcagca gcctgatcga gttggtgccg cagtcgattg cggcgactgt
     1681 gctcatgcct cggctcccgc tcgcatttgg ggcgtcatgt ccacacctcg cgatccagaa
     1741 tgccggccat gcccggctcg acggccagca tagccagcgc ctcgtcgccg aacgggttca
     1801 caccaggacc ttttgccagc gaatgcgcga tcaagacgtg gaggcacttc acccgttccg
     1861 gcatgcctcc gccggaaaac gtggtgccca gcgattcgat cgcgtcgcgc tcggccagat
     1921 aggactcgtg cgcgcgccgg tatgcggcag ccagttgcgg gtcgtcgtgc agtcgatcgg
     1981 tcatctcacg catcagcccg gatgactcca gacggctgcc cgccgccgtc agcgccgggt
     2041 gggtcaggta gtacagcgtc ggaaacggcg tgccgtcagg caatctcggg gccgtcttga
     2101 cgacgcctgg ctccccgttc gggcatcgat aggcgatctc caggacgccg cggggctcgc
     2161 ggcccaattg ccgcgcgacg gcatcgagat ccggccgctc aaccaccggg aggcggagca
     2221 tcgggcgggg cgggaggtgc gggtggaacg gagggcacag tggtcgggat gttgtgcgga
     2281 tcgtcggcga tcgtgtgcca cagcgacgtg taccacggct ggcccgcccg tgccgcggcg
     2341 gcttcccggg ccggctcacc gggcgccacc gcaccgggag gcagctgcac ctggtacggg
     2401 gtgtcgccgg gcttcacgaa gccgagccgc tcgcgcgcct gtgccgcaat gtaaacgggg
     2461 tcagcgagtt tcgccttctg ctcctcgagg ccggcgatct ccacgcgcag cttctcctcg
     2521 ctcgcggcca gctgcttcat ctcggttcgc tgggagaaat acgtccgcac ggggcccgca
     2581 atcgtcagcg tgagcacaca caccaccgcg gccagaatcg ccgcccgccg cgcagtcgaa
     2641 cccagccgct gttcggactg ctgctcggca gcctcggcga tcgacctgcg gatcgaatcc
     2701 gcggtcgtgg gctctacgtc gggctgcggc ttgggccgcg actcggcctt gcgcggctcg
     2761 cgtcgtgcgg gagaccctgc cctcggacgg ccccgtgcgg tgtcgccggc cttccccgac
     2821 ttggccgtct tggccggtcg ggacgccggg gaccgccgct tcgggtcggg ccgtttcgct
     2881 tcgggcacag gctatttggc ctcaccgtct acgcttcgca caagcggctc atcgccaggc
     2941 gcgaaccgcg gaaacgcgag gtcgcccgcg aatcgcgcgg catcgccgag cgtctcctcg
     3001 atgcgcagca actggttgta cttggcgacg cgctcgctgc gcgcgggtgc accggtcttg
     3061 atctgcccgc tgccgaccgc gaccgccagg tcggcgatcg tggtgtcctc ggtttcgccg
     3121 ctgcgatggc tcatcatggt gcggtagccg ctgttgtgtg ctagcgatac ggcatcgagc
     3181 gtctcggtca gcgtgccgat ctggttcacc ttgaccagca gagcgttggc cgcaccacgc
     3241 tcgatgccct cctcgagacg ttcggggttg gtgacgaaca gatcgtcgcc gacgatctgg
     3301 acacggtcgc cgatcgtggt cgtcaacgcc acccagccgt cccagtcgtc ctccgacagc
     3361 ggatcctcga tcgagacgag cggatacgcg tccagcagcc cggcgtagaa ctcgatcatc
     3421 tggtccgcgc ttcgcgtttg cttctcgaag ctgtaacccg ttccctcagt gtagaattcg
     3481 gttgccgcca cgtcgagcgc aagcgccacg tcggtacctg gcttgaagcc cgtcgcgttg
     3541 atggccgaca ggatcaggtc gagggcggct ttggtgtccg ccacgtcggg cgcgaatccg
     3601 ccctcgtcgc ccagtccggt ggccaggccc tgcttcttga ggaccgactt cagcgagtgg
     3661 tacacctcag tgccccagcg cagcgactcc ttgaagctcg gcgcaccgat cggcgcgacc
     3721 atgaactcct gaacgtccac gccggtgtcg gcgtgtgcgc cgccgttgag gatgttcatc
     3781 atcggcacgg gtaggacatg ggcgttcggt ccgccgacgt agcggaacag cggcagcccc
     3841 gccgattccg cggcggcctt ggccaccgcc agcgacacgc cgaggatcgc gttggcgccc
     3901 aaccgcgact tgtccggtgt gccgtcgagg tcgagcaggg cctggtcgat cacccgctgg
     3961 tcgtcggcgc tcaacccgat gaccgtgggc gcgatctcgt cgagaaccgc ctcgacggcc
     4021 ttgcgcacac ccttgccgcc gtagcgcgat ccgccgtcgc gcagctccac cgcctcgtgc
     4081 tcaccggtcg aggcgcccga cggcaccgcc gccctggcga acgtgccgtc ggtcagggcc
     4141 aactcgacct cgaccgtcgg gttgccgcgg gagtcgagga tttcgcgtgc tccgacctgc
     4201 tcgatgatgg gcactggcgt tctccttggt cggtcgagtt cggactacgg gcctagggga
     4261 tgagcctagt cgggcgacga tcaagcggcg gggcgccgcg ttgtgggggt acctccctcg
     4321 cgaagcgtag ggggagagct cgacaatccg gcctagaacg tgctcatggg gacagtgcga
     4381 tcaaaggggg tggccatcgg cgtaggccgt cgcccagtcc cgcacggtgc gggcgtactg
     4441 gtcggacaag ttgtaggccc gcagagcgtt catccagcct cgtggcgtgg acagattctt
     4501 gccgcgccag cacagatagc ccgccgccga gagcgccgcg tcgtcgaagt tgtcggggct
     4561 gaccttgccg tcgttgttcg cgtcgacccc gtagagccgc cacgtttccg ggatgaactg
     4621 catggggccc atcgcccggt cgataccggg atcgccgtcg agcagaccag ag
//
LOCUS       DASYLX010000563         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1014759,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000563 DASYLX010000000
VERSION     DASYLX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014759"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1762)
                     /locus_tag="VGP27_13565"
     CDS             complement(20..1762)
                     /locus_tag="VGP27_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7852360.1"
                     /translation="MLLALLRQYVRPYRWLLITVAVLQVISTLASLYLPTVNAAIIDD
                     GVAKGDTHTIVELGGVMLAVTGLQVLCAIGAVYFGSRSAMGFGRDLRSAIFHHVTGFS
                     AAETARFGAPTLLTRTTNDVQQVQLLVQLTCTMLITAPIMSIGGIFMAVHQDAGLSWL
                     LLVSVPVLALANYWIVSHLLPIFRSLQRLIDGINRVMREQLSGIRVIRAFAREPFERS
                     RFAEANQALSDTALTAGRWQALMLPVTTLVINVSSVALIWFGGHRIDEGEMQVGSLIA
                     FLSYFMQILMAVLLATFILVLVPRAAACAERITEVLCTEPAITSPAEPVRPASVDGAI
                     SLRDATFCYPGADRPVLQNVSLTAGPGTTTAIVGSTGSGKSTLVSLICRMYDVTSGSV
                     RIDDIDVRDYDTEQLWSAIGLVPQRGYLFSGTVAENLRYGKADATDDEMWEALRVASA
                     DGFVASHADGLDMPVAQGGINFSGGQRQRLAIARAVIRRPAIYLFDDAFSALDVHTDA
                     RVRSALSDVSADATVVIVAQRISTVAAADQIVVVDDGEVVGTGTHESLLLDCPTYAEF
                     ADSQAIGAGVGGTP"
     gene            1889..2434
                     /locus_tag="VGP27_13570"
     CDS             1889..2434
                     /locus_tag="VGP27_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEV7852361.1"
                     /translation="MPGNVKRLAVAAAVLIPMLAACSSGSNSSPNPSEGQGPAQGVKH
                     GPFFPQCGGISDQTMAEQSRVPGLVNTAKNSVGCQWLAGGGILGPHFSFTWYRGSPIG
                     RERKTEELSRSSVDPINIEGHDGFIAIGQDPTLGQNLCEIGIQYDDDFIEWSISFSEK
                     PFPDPCDVAKELTRQTIVNAK"
     gene            2431..>2967
                     /locus_tag="VGP27_13575"
     CDS             2431..>2967
                     /locus_tag="VGP27_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEV7852362.1"
                     /translation="MTGSVRRVAAAIVAVLAAVTMLVGCSRTVEGTAAKAGSGDVPRN
                     DNSQKQYPNLLKECEVLTEDILAKTVGADPLDIQSTFVGAVCRWQAANPAGLIDITRF
                     WFEQGSLDNERKTADQLGYKVESRAIAGVPSIVMHPNDPNGACGVASDAGGVVGWWVN
                     PQQPGIDACGMALKLMELT"
BASE COUNT          544 a          982 c          986 g          455 t
ORIGIN      
        1 gggcgcccca gcggctgtgt catggagtgc ctccgacgcc ggctccgatt gcctgcgagt
       61 cggcgaactc ggcataggtg gggcagtcga gcagcaacga ctcgtgggtt cctgtgccca
      121 ccacctcgcc gtcgtcgacg acgacgatct gatcggccgc ggccaccgtc gaaatccgtt
      181 gcgccacgat gacgaccgtg gcgtcggcgg acacgtcgct cagcgccgac cggacccggg
      241 cgtcggtgtg tacgtcgagc gccgagaacg cgtcgtcgaa caagtagatg gccggtctac
      301 ggatcactgc gcgggcgatt gccaatcgct gccgctgacc gcccgagaag ttgatgccgc
      361 cctgggcgac gggcatgtcg aggccgtcgg cgtgggaagc gacgaatccg tcggccgacg
      421 ccacccgcag cgcctcccac atctcgtcgt cggtcgcgtc ggccttgccg tagcgcaggt
      481 tttcggcgac ggtgccggag aacaggtaac cccgctgcgg cacaaggccg atcgcggacc
      541 acagctgttc ggtgtcgtag tcccgcacgt cgatgtcgtc gatgcgcaca gatccagagg
      601 tgacgtcgta catccggcag atcagcgata ccagcgtcga cttgcctgaa ccggtggacc
      661 cgacgatggc ggtggtcgtg ccgggccctg ccgtcagcga gacgttctgc agcacaggac
      721 gatcggcgcc cggatagcag aacgtggcgt cgcgcaggct gatcgccccg tcgaccgacg
      781 ccggccggac gggctcggcg gggctggtga tcgccggctc ggtgcacaac acctcggtga
      841 tccgttccgc acacgccgcc gcccgcggca ccagcaccag gatgaacgtc gccagcagca
      901 cggccatcag gatctgcatg aagtaggaca ggaacgcaat caatgacccg acctgcatct
      961 cgccctcgtc gatgcgatgc ccgccgaacc agatcagcgc gacgctggag acgttgatca
     1021 ccagcgtggt caccggaagc atcagcgcct gccaacggcc cgccgtcaaa gcggtatcgg
     1081 acagcgcctg gttggcttcg gcgaatcggc tgcgctcgaa cggctctcgg gcgaaggcgc
     1141 ggatcactcg gatacccgac agctgttcgc gcatcacccg gttgatgccg tcgatgagcc
     1201 gctgcaggct gcggaagatc ggcagcagat gcgacacgat ccagtagttc gccagcgcca
     1261 gcacgggaac gctgacaagc agcagccacg acagtccggc gtcctggtgc accgccatga
     1321 agatgccgcc gatcgacatg atcggcgcgg tgatgagcat cgtgcatgtc aactgcacca
     1381 gcagctgcac ctgctggacg tcgttggtgg tgcgggtcag cagagtcggt gcgccgaagc
     1441 gtgcggtctc ggcggccgag aacccggtca cgtggtgaaa gatcgccgac cgcagatcgc
     1501 gaccgaaacc catcgcggag cgcgagccga agtacaccgc gccgatggcg cacagcacct
     1561 gcagtccggt gacggcgagc atcaccccgc caagctcgac gatggtgtgg gtgtcgccct
     1621 tggccacgcc gtcgtcgatg atggccgcat tgaccgtcgg caggtacagc gacgcgagtg
     1681 tgctgatcac ctgcagcacc gcgacggtga tgagcagcca gcggtacggc cgcacatatt
     1741 gccgcagcag agccaggagc atccagctac tttcgcacac catcctgtcg ggttatcgac
     1801 gccccgagac ccgttctgaa cgagaaaaag gcaggtcagc gtgtctctca ggatgacctt
     1861 ggtggctgcc gctacagtgt gcgctgtgat ccccggcaac gtgaagcggc tggccgtcgc
     1921 agcggccgtc ctgatcccga tgttggcggc ttgctcttcg ggctcgaact cgtcgcccaa
     1981 cccgtcggag ggtcaagggc ccgcgcaggg agtcaagcac ggccccttct ttccccagtg
     2041 cggcggtatc agcgatcaga cgatggccga gcagagccgc gtgccgggcc tggtcaacac
     2101 ggccaagaat tcggtcggat gccagtggct ggccggcggc ggcatcctcg gcccgcattt
     2161 ctcgttcacc tggtaccgcg gcagcccgat cgggcgcgag cgcaagaccg aagagctctc
     2221 ccggagcagc gtcgacccca tcaacatcga aggccatgac ggcttcatcg ccataggcca
     2281 ggacccgacg ctggggcaga acctctgcga gatcggcatc cagtacgacg acgacttcat
     2341 cgagtggtcg atcagcttct cggaaaagcc cttcccggat ccgtgcgatg tggccaaaga
     2401 gttgacccgt cagacgatcg ttaacgccaa atgaccgggt cagtgcgccg ggtggccgcc
     2461 gcgatcgtcg ccgtgctggc cgcggtgacg atgctggtcg gatgttcgcg aaccgtcgag
     2521 ggcaccgcag ccaaggccgg ttccggcgac gtgccgcgca acgacaactc gcagaagcag
     2581 tatccgaacc tcctcaagga atgtgaggtc ctcaccgagg acatcctggc caaaacggtc
     2641 ggtgccgacc cgctcgatat tcagagcacc ttcgtcggcg cggtgtgccg ctggcaggcc
     2701 gccaaccccg ccgggctgat cgacatcacc cggttctggt tcgaacaggg cagcctggac
     2761 aacgagcgca agaccgccga ccagctcggg tacaaggtcg agagccgcgc gatcgcaggc
     2821 gttccctcga tcgtcatgca tcccaacgac ccgaacggcg cgtgcggcgt ggccagcgat
     2881 gcgggcggcg tggtgggctg gtgggtcaac ccgcagcagc cgggcatcga cgcatgcggg
     2941 atggccctca agctgatgga gctgacg
//
LOCUS       DASYLX010000564         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1783185,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000564 DASYLX010000000
VERSION     DASYLX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783185"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="VGP27_13580"
     CDS             complement(<1..101)
                     /locus_tag="VGP27_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEV7852363.1"
                     /translation="MPDRDPDTIKQDIDQARDQLAITVDTLAERANPR"
     gene            255..728
                     /gene="bcp"
                     /locus_tag="VGP27_13585"
     CDS             255..728
                     /gene="bcp"
                     /locus_tag="VGP27_13585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEV7852364.1"
                     /translation="MAQTPRLEAGDKAPAFSLPDADGNTVSLADYRGRKVIVYFYPAA
                     STPGCTKEACDFRDNLGELNEAGLDVVGISPDKPEKLAKFRDNEKLTFPLLSDPDRKV
                     LTQWGAFGEKTMYGKTVQGVIRSTFVIDEKGKIDVAQYNIKATGHVAKLRRDLSV"
     gene            complement(770..2104)
                     /locus_tag="VGP27_13590"
     CDS             complement(770..2104)
                     /locus_tag="VGP27_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV7852365.1"
                     /translation="MSDLVERVRDVLPSVRHDLEDLVRIQSVGADPARRAEVQRSAEA
                     VAKLLSEAGFGEVQIVSEGGAPAVIARHPAPSGAPTVLLYAHHDVQPEGDHTQWVSPP
                     FEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGQPPVGVTVFVEGEEETGSPSLGRL
                     LAAHKDTLAADVIVIADSDNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVV
                     PDALSVLVRLLASLHDDDGNVAVEGLHEATAADVDYPKDRVREESGMLTGVAEIGSGS
                     VPQRLWAKPAITVIGIDTTPIASSSNTLIPRARAKVSMRVAPGGDARAHLEALTRHLN
                     EHVPWGAQLTVSPGDLGQPYAIDASGPVYEAARDAFRQAWAAEPVDMGMGGSIPFIAE
                     FADTFPSAKILVTGVEDPGTQAHSINESLHLGVLEHAATAEALLLANLASRT"
     gene            complement(2256..2648)
                     /locus_tag="VGP27_13595"
     CDS             complement(2256..2648)
                     /locus_tag="VGP27_13595"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV7852366.1"
                     /translation="MSIVGVGIDLVSITEFAEQVDRPGTVFAETFTPGERRDAADKSS
                     SAARHLAARWAAKEAVIKAWSGSRFSRRPVLPEGIHRDIEVITDMWGRPRVRLTGAIA
                     EHLKDVTIHLSLTHEADTAAAVAVLEER"
     gene            complement(2659..>3592)
                     /locus_tag="VGP27_13600"
     CDS             complement(2659..>3592)
                     /locus_tag="VGP27_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HEV7852367.1"
                     /translation="MAATADTETMRARGISDSKFSRANDRRRLGFLESQGGGTILLAR
                     GDLALKMGLPVLAVVGYAQSFADGVHTSSPAPGLGALGAGRGGKDSLLSRSLAQLGVG
                     ADDIAVVSKHDTSTLANDPNETELHERLADSMGRSDGAPMFVVSQKSLTGHAKGGAAV
                     FQMMGMCQMLRDGVIPPNRSLDCVDDELAGSEHFVWVRETLRLGEKFPLKAGLITSLG
                     FGHVSGVIALVHPQAFVAALDPEQRANYQEQANARVFAGQRRLASAIAGGKPMYERPA
                     SNRRLDDEAPENRAEAEMLLNPASRLGEDGVYVR"
BASE COUNT          580 a         1302 c         1145 g          565 t
ORIGIN      
        1 cgcggattgg cccgttctgc gagcgtatcg acggtgatgg ccaactggtc acgagcctga
       61 tcgatgtcct gcttgatggt gtcgggatct cgatctggca ctgtcgtcct ccatcgtcag
      121 cgtgggcggg agcagttgtg cccctcgcgt ccgtgcggta gccctgtacg cctcgcgtcc
      181 gcaccgcctt gccgaactac cctagatcag ccggcgccaa cctcgacgca ccggtggaga
      241 gaaggaatca accggtggca cagaccccac gcctggaggc gggcgacaaa gcccccgcgt
      301 tcagcctgcc cgacgccgac ggcaacacgg tatcgctggc cgactaccgc ggccgcaaag
      361 tcatcgtcta cttctatccg gcggcgtcga cgcccggatg taccaaggag gcgtgcgact
      421 tccgcgacaa cctgggcgaa ttgaacgaag ccggcctcga cgtcgtcggc atctcccccg
      481 acaagccgga gaagctggcc aagttccgcg acaacgagaa gctcaccttc ccgctgttgt
      541 cggacccgga ccgcaaggtg ctgacgcagt ggggcgcctt cggcgagaag acgatgtacg
      601 gcaagaccgt gcagggcgtc atccggtcca cgtttgtcat cgacgagaag ggcaagatcg
      661 acgtcgcgca gtacaacatc aaggccacgg gccacgtggc caagctgcgt cgcgatctat
      721 cggtctgacc tctctctgct cctcacgtcc gcgacttacc ctctgctcct cacgtccgcg
      781 acgccaggtt cgccagcagc agcgcttcgg ccgtggcggc atgctccagc accccgagat
      841 gcaggctctc gttgatgcta tgcgcttgtg tcccagggtc ttccacaccg gtgaccagga
      901 ttttcgccga cgggaacgta tcggcgaact cggcgatgaa cgggatcgac ccgcccatgc
      961 ccatgtccac cggctcggcg gcccaggcct gccggaacgc atcgcgcgcc gcctcgtaga
     1021 cgggtccgct cgcgtcgatc gcgtacggct gcccgaggtc gccggggctg acggtgagct
     1081 gcgcgcccca cggcacgtgt tcgttcaggt gacgggtcaa cgcctcgaga tgggcacgcg
     1141 cgtcgccgcc cggcgccacc cgcatgctga ccttcgcccg cgcccgcgga atgagcgtgt
     1201 tcgacgacga tgcgatcggc gtggtgtcga ttccgatgac cgtgatcgcc ggtttcgccc
     1261 aaagccgttg cggcacagac ccggagccga tctcagcgac acccgtcagc attcccgatt
     1321 cttcgcgaac ccggtccttc ggatagtcca cgtcggccgc ggtcgcctcg tgcagaccct
     1381 ctacggcgac attgccgtcg tcgtcgtgca gcgacgccag caatcgcacc agcacgctca
     1441 gcgcgtcggg caccacaccg ccccacaggc ccgagtgcag accgtggtcc agcgtggcca
     1501 cctccaccac gcagtcgacc agaccgcgca gcgacaccgt caacgccgga acgtcggtgc
     1561 tccagttgtc ggagtcggcg atgacgatga cgtcggcggc cagcgtgtcc ttgtgcgcgg
     1621 ccagcagccg acccagcgac ggcgagccgg tctcctcctc gccttcgacg aacaccgtca
     1681 caccgaccgg cggttgaccg ccgtgcgccc gaaatgcggc gagatgcgtt gcgatgccgg
     1741 ccttgtcgtc ggcactgccg cgaccgtaaa ggcgcccgtc acgctcggtc ggctcgaagg
     1801 gcggcgacac ccattgagtg tggtcaccct cgggctgcac atcgtgatgt gcgtacagca
     1861 gcaccgtcgg cgcgcccgaa ggcgcaggat gtcgcgcgat gacggccggt gcgccaccct
     1921 cgctgacgat ctgcacctca ccgaacccgg cttccgacaa cagctttgcc accgcctcgg
     1981 cgctgcgctg cacctcggct cgccgcgccg ggtcggcgcc caccgactgg atacgcacca
     2041 ggtcttcgag gtcgtgtcgc accgacggca gcacatcacg tacccgttcc acgagatcgc
     2101 tcatggtttc gaggctagcc gctcaggtct gcaccgcgag cgtgcacaga atgccagaaa
     2161 agcgcggcgt gtcgtgtaca aacacgcgcg ctcgcggggc gaggtgcccc agctcggggc
     2221 gaggtgcccc agctcggggg gcgatcgggg cgaggttaac gttcctcgag gacggcgacg
     2281 gccgccgcgg tgtcggcttc gtgggtcagc gagaggtgaa tggtcacatc cttcaaatgc
     2341 tcggcgatcg caccggtcag ccgcacccgc ggcctacccc acatgtcggt gatgacctcg
     2401 atgtcgcgat ggatgccctc agggagcacc ggccgcctgg agaaccggga ccccgaccac
     2461 gccttgatca ccgcctcctt ggccgcccag cgggccgcca agtgccgcgc cgccgacgaa
     2521 ctcttgtccg cggcgtcgcg gcgctcaccg ggtgtgaacg tctcggcgaa taccgtgccc
     2581 ggccggtcga cctgttctgc gaactcggtg atcgaaacca ggtcgatgcc cacgccgacg
     2641 atgctcacgg tgcgaaggtt aacgcacgta gacgccgtcc tcgcccagcc ttgatgcggg
     2701 attcagcagc atctccgcct ctgcgcggtt ctccggcgcc tcgtcgtcga gtcgacggtt
     2761 gctcgcaggc cgctcgtaca tcggcttgcc accggcgatc gccgacgcca gccgccgctg
     2821 ccccgcgaac acccgcgcgt tggcctgctc ctggtagttc gcccgctgct cgggatcgag
     2881 cgccgcaacg aaggcttgcg gatgcaccag cgcgatgacg cccgacacgt gaccgaagcc
     2941 gaggctggtg atcaggcccg ccttcagcgg gaacttctcc cccagccgca gcgtctcgcg
     3001 cacccacacg aagtgctcgg agcccgccag ctcgtcgtcg acgcagtcca ggctgcggtt
     3061 cggcgggatg accccgtcgc gcagcatctg gcacatgccc atcatctgga acaccgccgc
     3121 gccgcccttg gcgtgaccgg tcaggctctt ctgcgacacc acgaacatcg gcgcaccgtc
     3181 cgagcggccc atcgagtcgg ccagccgctc gtgcagctcc gtctcgttcg ggtcgttggc
     3241 gagcgtggag gtgtcgtgct tggacaccac cgcgatgtcg tcggcgccga cgccgagctg
     3301 ggcaagcgac cgggacagca gcgaatcctt cccgccccgg cccgcaccca gagcgccgag
     3361 cccgggagcc ggactggagg tgtgcacacc gtcggcgaac gactgcgcgt agcccaccac
     3421 ggccagcacc ggcagaccca tcttcagcgc caggtcacca cgcgccagca ggatcgtgcc
     3481 gccgccctgc gactccaaaa agcccagccg gcgacggtcg ttggctcggg agaacttcga
     3541 gtcgctgatg cccctggcgc gcatcgtctc ggtgtcggcg gtggccgcca tg
//
LOCUS       DASYLX010000565         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1783255,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000565 DASYLX010000000
VERSION     DASYLX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783255"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGP27_13605"
     CDS             complement(<1..369)
                     /locus_tag="VGP27_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEV7852368.1"
                     /translation="MPSQKTYLKVCINGARTPDQHPNLPVSPEQLAAAAVAAHQAGAK
                     AVHMHPKTADGVDSLQPEVVGAAVEAVRHAVPGLPVGVTTGFWALSDPDERLRTVEGW
                     TVLPDFASVNWHEPGSEPLAH"
     gene            421..2118
                     /locus_tag="VGP27_13610"
     CDS             421..2118
                     /locus_tag="VGP27_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV7852369.1"
                     /translation="MRGHTIVCGDDALGLRIVEELTTAGMSVVTLQSPDALSAAGVTD
                     AEAVICADDDDDLNLEIALLARQANPDVRVVARLGNGVLREAMAQDNGPGAILDVADL
                     AAPSVVEACLARTTHTISLAGVDFIVSGTDAPRDGTLREIYGDLAPVAVVYGETSATP
                     GEVVACPGRDFRVQEGDWTAMIGTAEELAAQNISVAKPIRSTRRRRPPIRRAIDAVRA
                     FREDLNPMFYRALAASSVLLLLATVLLRFAYQRPPGMSWIDALYFSTETIATVGYGDF
                     SFMDQPTWLRLFSILLMFAGVTTTAILVAFVADVLLSRRLAHSAGRRRVRHLHDHIIV
                     VGLGSFGIRVVGDLAAAGYDVVVIERKDDNRFLSTAARLDVPVIFGDATLRETLESAR
                     IDDARAVAVLTQDDIANIETGIVLHEMLGHRKVPIVLRVYDRTLGAAVAQRFGFENVR
                     STVDLAIPWFIGAALGLDVLGTFLVGQRSFMVGGVYVQPGSDLDGIKMADLSTQTRVI
                     AITRPGSPVRLHPRRDTKLSAGDTAYLVGPYRELLDTLRKGQGVQQTTDVTDPATTG"
     gene            complement(2093..2878)
                     /locus_tag="VGP27_13615"
     CDS             complement(2093..2878)
                     /locus_tag="VGP27_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7852370.1"
                     /translation="MPTRQGAPVGAPTWIDLATSDLERAKQFYGNVFGWTFESAGPEY
                     GGYVNAFSGGHPVAGLMTNDPQWNSPDGWTTYLHTADIRATLATAIEAGAVTCVQPVE
                     PMEVKDKGWMAMLVDPTGAFFGLWQPIEHRGFEVFGEACAPIYHQLTSRDYAKALDFY
                     RRVFGWQIESVSDTDEFRYSTANFDGEALVGIMDGTTITMPEGAPSNWFFFLGSDDVD
                     KTVALVRDNGGSVIRDAEDTPYGRLAAVADPTGAGFNLSSLDQ"
     gene            complement(2964..>3117)
                     /locus_tag="VGP27_13620"
     CDS             complement(2964..>3117)
                     /locus_tag="VGP27_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852371.1"
                     /translation="QLRTKVLFGTDYPYIEVDRWRRDFETLDMAPEVLPLIFKENALR
                     VLGVTC"
BASE COUNT          498 a         1045 c         1014 g          560 t
ORIGIN      
        1 atgcgccaag ggctcagatc ccggctcgtg ccagttcacc gacgcgaaat ccggcagcac
       61 cgtccagccc tcgaccgtgc gcagccgttc gtccggatcg gacagtgccc agaagccggt
      121 cgtcaccccg accggtaggc ccggaacggc atgccgcacc gcctccaccg ccgccccgac
      181 gacctcgggc tgcagcgagt cgacaccgtc ggcggtcttg ggatgcatgt gcaccgcctt
      241 cgcgccggcc tgatgcgcgg cgacggccgc cgccgcgagt tgctcggggc tcaccggcag
      301 attcggatgc tgatccgggg tcctggcgcc gttgatgcat accttgaggt aggtcttctg
      361 gcttggcatg cgtttatctt cacccttcgg gcacgcagtt cgcgcagtag cgtgcttccc
      421 atgcgtggcc acaccatcgt ctgtggtgac gatgcgcttg ggttgcggat cgtcgaggag
      481 ctcacgaccg cgggcatgag tgtcgtgacc ctgcagtctc ccgacgcact gtccgcggcc
      541 ggcgtcaccg acgccgaggc ggtgatctgc gccgacgacg atgacgacct gaacctcgag
      601 atcgcccttc tcgcccggca ggccaacccc gacgtacgag tggtggcccg cctcgggaac
      661 ggcgttcttc gcgaggcgat ggcgcaggac aacggcccgg gcgcgatcct cgatgtcgct
      721 gacctcgcgg caccgtccgt cgttgaggcg tgcctcgccc gcaccactca caccatctcg
      781 ctcgcaggcg tggatttcat cgtctccgga accgatgcac cacgcgacgg cacgctgcgg
      841 gagatctacg gcgatctggc acccgtcgcg gtggtctacg gcgagacgtc ggcaaccccc
      901 ggtgaagtgg tcgcatgccc gggacgtgac ttcagagttc aggaaggcga ctggacggcg
      961 atgatcggca ccgccgagga gcttgccgca cagaatattt cggtcgcgaa gccgatcaga
     1021 tcgactcgca gacgacgccc gccgattcga cgcgccatcg acgcggtgcg ggccttccgc
     1081 gaggacctca acccgatgtt ctaccgcgcg ctggcggcgt cgtcggtgct gctgttattg
     1141 gcgacggtgc tgttgcgctt cgcctaccaa cgtccgccag gtatgagctg gatcgacgcg
     1201 ctgtacttca gcaccgagac gatagccacc gtcggatacg gcgatttcag cttcatggac
     1261 cagccgacct ggctgcggct gttcagcatt ctgctgatgt tcgccggggt gaccaccacg
     1321 gcgatactcg tggccttcgt cgccgacgtc ctgctgtcgc ggcgactcgc tcactcagcg
     1381 ggacgtcggc gggtacgcca cctgcacgac cacatcatcg tcgtcggcct cggctcgttc
     1441 ggcatccgtg tggtgggtga tctggcggcc gccggctacg acgtcgtcgt catcgagcgc
     1501 aaggacgaca accgcttcct gtccacggcc gcccgactcg acgtgccggt gatcttcggt
     1561 gacgcgactc tgcgcgagac gctcgaatcg gcgcgcatcg acgacgcccg cgcggtcgcg
     1621 gtgctcaccc aggacgacat cgccaacatc gaaaccggca tcgtgctgca tgagatgctc
     1681 ggccaccgca aggtgccgat cgtcctgcgg gtctacgacc ggacgctcgg ggcggcggtg
     1741 gcccagcggt tcggcttcga aaacgtccgg tccacagtcg atctcgctat cccgtggttc
     1801 atcggcgccg cgctcgggct ggatgtgctc ggcaccttcc tggtggggca gcgctcgttc
     1861 atggtcggtg gcgtttacgt gcaaccgggc agtgacctgg acggcatcaa gatggcggac
     1921 ttgtcgacgc aaacgcgggt catcgccatt acccggcccg gcagcccggt tcgactccac
     1981 ccgcgacgcg acaccaagct cagcgccggg gacaccgcct acctggtggg tccgtatcgg
     2041 gaactgctgg acaccctgcg caaaggacag ggtgtccagc agaccaccga tgtcactgat
     2101 ccagcgacga caggttgaag cccgcaccgg tcgggtcggc gacagccgcc aaccggccgt
     2161 acggcgtgtc ctctgcatcg cggatcacgc tgccgccgtt gtccctgacc agtgccaccg
     2221 tcttgtcaac gtcatcgctg ccgaggaaga agaaccagtt cgacggcgca ccctcgggca
     2281 tagtgatcgt ggttccgtcc atgatcccga ccagcgcttc gccatcgaaa ttggcagtgc
     2341 tgtagcggaa ttcgtcagta tcggagacgc tctcgatctg ccagccgaac acccgccggt
     2401 agaagtccag cgccttggcg tagtcgcgac tggtcagctg gtgataaatc ggagcacagg
     2461 cctcgccgaa cacctcgaag ccgcggtgct cgatcggttg ccacagcccg aagaacgcac
     2521 cggttggatc gaccagcatg gccatccacc ctttgtcctt cacctccatc ggttcgaccg
     2581 gctggacgca agtgaccgcg cccgcttcga tggccgttgc aagcgtggcc ctgatgtcgg
     2641 cggtgtgcag gtaggtggtc cagccgtcgg ggctgttcca ctgcgggtcg ttggtcatca
     2701 ggcccgcgac ggggtggccg ccggagaagg cgttgacgta gccgccgtat tcaggtccgg
     2761 cggattcgaa cgtccagccg aatacgttgc cgtagaactg cttggcgcgt tcgaggtcgg
     2821 aggttgcgag gtcgatccag gtgggggcac ctacgggtgc gccttggcgg gtgggcacgt
     2881 tggagtctcc ttgtgctggt gtggtcggtc accgatgcag accgccgtcg cctggaaaac
     2941 tcatcacctc ttcagccgag cggttagcag gtgaccccga ggacccgcag cgcgttctcc
     3001 ttgaagatca gcggcagcac ctcgggcgcc atgtcgagtg tctcgaagtc gcgccgccac
     3061 cggtccacct cgatgtacgg gtagtcggtg ccgaacagca ccttggtgcg cagttgg
//
LOCUS       DASYLX010000566         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_905794,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000566 DASYLX010000000
VERSION     DASYLX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905794"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VGP27_13625"
     CDS             <1..1311
                     /locus_tag="VGP27_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV7852372.1"
                     /translation="EVLDKEYEGFSEGMKTNGFSAAAIKALWDTVLPFADYAFNKSHA
                     AGYGLVSYWTAYLKANYPAEYMAGLLTSVGDDKDKAAVYLADCRKLGITVLPPDVNES
                     GLNFASVGEDIRYGLGAVRNVGANVVASLIGSRTDKGKFIDFSDYLNKIDIGPCNKKV
                     TESLIKAGAFDSLGHPRKGLFLVHTDAVESVLGTKKAEAMGQFDLFGGADTATDAVFT
                     IKVPDEEWDDKHKLALEREMLGLYVSGHPLNGVAHLLATQVDTQIPAILDGDVPNDAQ
                     VRVGGILTGVNRRVNKNGMPWASAQLEDLTGGVEVMFFPHTYSSFGAEVVDDAVVLVS
                     AKVRIQDDRICLIANDLLVPDFSNAAVDRPLAVSLPTRQCTIDKVNALKQVLARHPGT
                     SQVHLRLISGERITTLELDQSLRVTPSSALMGDLKALLGPGCLG"
     gene            complement(1325..2065)
                     /locus_tag="VGP27_13630"
     CDS             complement(1325..2065)
                     /locus_tag="VGP27_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852373.1"
                     /translation="MDGSPESDARRERLTRFAQELVADAEKRVPAWVRHGDPESAIPV
                     RIAVLAVMAEQIAIPRAYTVVPRWPLLVLEVLLLVALLAINPRVMSRRTRFGRYATWA
                     LLAAITIDNTLSAILLDARIISGQVSNNAAVLLGSGAGIFVTNIIVFGVWYWELDRGG
                     PFARHIGERPYPDFLFPQMATPNVSKPDWRPTFVDYLYVSVTNVMAFSPTDTMPLARW
                     AKALMTLQAMVALSTAALVISRAVNVLG"
     gene            2097..>3707
                     /gene="fadD11"
                     /locus_tag="VGP27_13635"
     CDS             2097..>3707
                     /gene="fadD11"
                     /locus_tag="VGP27_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase FadD11"
                     /protein_id="HEV7852374.1"
                     /translation="MSTSDRPATLCEAFQRTAAIDPDAIALRTVGGTQTLTWREYAAQ
                     VRKVAAGLAGLGVRRGDTVSLMMSNRPEFYPVEVGAQHVGATSFSVYNTLPAEQLVYL
                     FDNAGTRVIMCEAQYVDRIRASGAEIDHIVCLDGAPDGTLSVDDLIAAGADDYDFEAS
                     WRAVRSDDVATLIYTSGTTGNPKGVEMTHANLLFQAFALDEVLGVRFGDRSTSFLPTA
                     HIADRMGALYLQEVFGTQITTVADPRAIAGALPDVRPTIWGAVPRVWEKLKAAIEFAA
                     ANEPDETKRIGLQWGLSVAAKKADALLAAKPVPDDVAAEWAQADELVLSKLREKLGLE
                     QVRHTVSGAAPIPKETLAFFLGLGIPISEVWGMSELSCIATVAHPSDTRLGTVGKLVP
                     GMEGRIAEDGEFLVRGPLVMKGYRKEPRKTAEAVDEDGWLHTGDVMEVDQDGYLKIVD
                     RKKELIINAAGKNMSPANIENTIKAACPLIGVILAIGDARAYNTALIVLDAESAGPYA
                     AQRGLADDSAEALAAHPDVISEIAAGVAA"
BASE COUNT          697 a         1169 c         1226 g          615 t
ORIGIN      
        1 gaagtgctcg acaaggagta cgagggcttc tcggagggca tgaagaccaa cgggttctcg
       61 gcggccgcca tcaaggcgct gtgggacacc gtgcttccgt tcgccgacta cgctttcaac
      121 aagtcgcacg ccgcaggcta cgggctggtg tcgtactgga cggcctacct caaggcgaac
      181 tacccggccg agtacatggc cggactgctc acgtcggtgg gtgacgacaa ggacaaggcg
      241 gcggtgtacc tcgcggactg ccgcaaactc ggcatcaccg tgctgccgcc ggatgtcaac
      301 gagtccgggc tgaacttcgc ttccgttggc gaggacatcc gctacgggct cggcgccgtc
      361 cgcaacgtcg gcgcgaacgt cgttgcctcg cttatcggtt cgcgaaccga caaggggaag
      421 ttcatcgact tctccgacta cctgaacaag atcgatatcg ggccgtgcaa caagaaggtc
      481 accgagtcgt tgatcaaggc aggcgcgttc gactcgctcg gccatccgcg caagggtctc
      541 ttcctcgtgc acaccgacgc cgtcgaatcg gtgctcggaa ccaagaaggc cgaggcgatg
      601 ggccagttcg acctcttcgg cggggcggac accgccaccg atgcggtgtt caccatcaag
      661 gtgcccgacg aggagtggga cgacaagcac aagctggcgc tggaacggga gatgctcggc
      721 ctgtacgtgt ccggccatcc gctcaacggc gtcgctcacc tgttggcgac gcaggtggat
      781 acccagattc cggccatcct cgacggcgac gtccccaacg acgcccaggt gcgggtcggc
      841 gggattctga ccggggtgaa tcgccgcgtc aacaagaacg ggatgccatg ggcttcagct
      901 caattggaag acctgaccgg tggcgtcgag gtgatgttct tcccgcatac gtactcgagt
      961 ttcggcgcgg aagtcgtcga cgacgcggtg gttctcgtca gcgccaaggt cagaattcag
     1021 gacgaccgga tctgcctgat cgccaacgac cttctggtgc ccgacttctc gaatgccgcc
     1081 gtggaccggc cactggcggt gagcctgccg acgcgacagt gcaccatcga caaggtcaac
     1141 gcactcaagc aggtgcttgc gcgacacccg ggcacctcgc aggtgcatct gcggctgatc
     1201 agcggggagc gcatcaccac gctggaactg gaccagtcgc tgcgcgtcac gccgtcgtcg
     1261 gcgctgatgg gtgacctgaa agcgctgctc gggccgggct gcctcggcta gattgcgtgc
     1321 tggttcagcc caaaacattt accgccctcg agatcacgag tgccgctgtg gatagagcga
     1381 ccatcgcctg aagggtcatc agcgcctttg cccagcgtgc cagcggcatg gtatcggtgg
     1441 gggagaacgc catcacgttg gtgacgctga cgtacaggta gtcgacgaac gtcgggcgcc
     1501 aatccggttt gctcacattg ggagtcgcca tctgagggaa cagaaagtcg ggatacggtc
     1561 gctctccgat gtgcctggcg aacgggccac cgcggtcgag ctcccagtac cagaccccga
     1621 acacaatgat gtttgtgacg aagatccccg caccgctacc gagaagcacg gccgcgttgt
     1681 tgctcacctg accggagatg atgcgggcgt ccaacaaaat cgcagacagt gtgttgtcga
     1741 tggtgatcgc cgcgagaagc gcccaggtgg cgtacctgcc gaaccgcgtt cggcgcgaca
     1801 tgacgcgtgg attgatcgcc agcagcgcga ccagaaggag aacctcgagc acaagcagtg
     1861 gccaacgcgg caccaccgtg taagccctcg gtatggcgat ctgctccgcc atcacggcga
     1921 gcacagcgat gcgtaccggt atcgcactct cggggtcgcc gtggcgaacc catgcgggta
     1981 ctcgcttctc ggcgtcggcc acgagctcct gggcgaaccg ggtcaggcgt tcgcggcgtg
     2041 catcagattc cggtgaaccg tccatctgca cagtgtgatt gcctaagttg tgaaccatga
     2101 gcacatccga ccggcctgcc acgctctgtg aagctttcca gcgcaccgcc gcgatcgatc
     2161 ccgacgcgat cgccctacga acggtgggcg gcacccaaac gctgacctgg cgtgaatatg
     2221 ctgctcaggt gcggaaggtc gccgcaggac tggccggcct cggcgtccgg cgcggcgaca
     2281 cggtgtcgct gatgatgtcc aatcgcccgg agttctatcc agtcgaggta ggcgcacaac
     2341 atgtcggtgc gacctcgttc tccgtctaca acacgttgcc ggccgagcag ctggtgtacc
     2401 tgttcgacaa cgccggcacc agggtgatca tgtgtgaagc gcagtacgtc gaccggatcc
     2461 gggccagcgg tgccgagatc gaccacatcg tgtgcctcga cggcgcacct gacggcacgc
     2521 tgtcggtcga cgacctcatc gccgccggcg cggacgacta cgacttcgag gcgtcctggc
     2581 gtgcggtgcg gtccgatgac gtcgccacgc tgatctacac gtcgggtacc accggcaatc
     2641 ccaagggcgt ggagatgacg cacgccaacc tgctgttcca ggcgttcgcg ctcgacgagg
     2701 tgctcggggt gcggttcggg gaccgcagta cgtcgttcct gccgacggcc cacatcgccg
     2761 accggatggg cgcgctgtat ctgcaggagg ttttcggcac ccagatcacc accgttgcag
     2821 acccgcgtgc catcgccgga gcattgccgg acgtgcggcc gacgatctgg ggcgcggtac
     2881 cccgggtatg ggaaaagctc aaggcggcaa tcgaattcgc ggccgccaac gagcccgacg
     2941 agacgaagcg gatcgggttg cagtggggcc tgtcggtggc cgcgaaaaag gccgacgccc
     3001 tactggccgc gaagccggta cccgatgacg ttgccgcgga gtgggcgcag gccgacgaac
     3061 tggtgctgtc gaagctgcgc gagaagctgg gtctggaaca ggtcaggcat acggtgtcgg
     3121 gcgcggcgcc gatcccgaag gaaaccttgg cgttcttcct cggcctggga attccgatct
     3181 ccgaagtctg gggcatgtcg gagctgagct gcatcgcaac ggtcgcacat ccctccgaca
     3241 cccgactggg cacggtgggc aagctcgtgc ccggcatgga gggccggatc gccgaggacg
     3301 gcgagttcct tgtgcgcggc ccgctggtga tgaagggcta ccgcaaagag ccgaggaaga
     3361 cggcggaggc cgtcgacgaa gacggctggc tgcacaccgg cgatgtgatg gaggtcgacc
     3421 aggacggcta cctgaagatc gttgatcgca agaaagaact gatcatcaac gcggccggaa
     3481 aaaacatgtc gccggccaac atcgagaaca ccatcaaggc cgcgtgcccg ctcatcggcg
     3541 tgatcctcgc catcggcgac gcccgtgcgt acaacaccgc gctgatcgtc ctggacgccg
     3601 agtccgctgg gccgtatgcg gcgcaacgcg gattggccga cgattcggcg gaggctctgg
     3661 ccgcgcaccc cgacgtgatc tccgagatcg ctgcaggcgt cgcagcc
//
LOCUS       DASYLX010000567         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1453448,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000567 DASYLX010000000
VERSION     DASYLX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453448"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGP27_13640"
     CDS             <1..229
                     /locus_tag="VGP27_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohex-1-ene-1-carboxylate:CoA ligase"
                     /protein_id="HEV7852375.1"
                     /translation="GRPDELRGEMVCAVVRRSARHRDVTLHELCAFLDERGLMKQKWP
                     ERLHFVDEFPLTGLGKVAKAELAQLISGGSQ"
     gene            280..1431
                     /locus_tag="VGP27_13645"
     CDS             280..1431
                     /locus_tag="VGP27_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852376.1"
                     /translation="MTVLSVDERDELARSVRAACEKFASQERVRAVAYDDPEDARGGF
                     DSGLWEVLCSQVGVAAIGLPEHLGGAGYGASALGVVAHELGRVLAPVPFVASTVLATG
                     LLLDLQGPDGGAEERFSGLLEGRRTAAAAITGDGGLWRRTAVTLTATEGHDDSWTVDG
                     VVRHVLNGTTADDLVVVATTNGELALFLIDPATDGVEVDAERVLDGTRPMATLAFTGV
                     PALKLSSDGPVDDIIERNVDLALAVLSAEQVGACERVLEIATEYARTRVQFDRAIGSF
                     QAVKHRCADMLVNLEWARSASAAALESVDADAKEMAGESDWRASMAKAVCSEALRDAA
                     HANVQIHGGIGFTWEDSAHLYLRRARTDEVLFGGPGQHWDRLAIRKKLL"
     gene            1559..2710
                     /locus_tag="VGP27_13650"
     CDS             1559..2710
                     /locus_tag="VGP27_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852377.1"
                     /translation="MPLQPYMELISVDDHLIEHPKVWSDRLPKKYLEAGPKIIEWERP
                     DTGQMCQVWDYEGRIYPYIGLNAVTGKKPEEYGIEPVRYDDMIPGCYDPKARVADMDI
                     DGVQAMTCFPSFPRFAGAVFAEGEDKELAKLCSAAWNDFHLDEWAATAPDRFIPVAML
                     PFWDLDASVKEIHRVAAKGAKCVTFPDLPDKLGLPSVHSDHWDPLLSAMEETGLVLAQ
                     HFGSGGFPPPIAPDAPFAVFVTLMGTMSMTALTDWLFSHTLYRHPDLKIGLSEGGIGW
                     IPYILERADSVWRKHRFYNNVNQDHEPSYLWKKHFHGCFIEDDFGVAVRHAVGVDRIT
                     WECDYPHSDSFWPNSRDRAAEALKDVPDDEVHQMVELNARGFYNFPRAA"
     gene            2721..2975
                     /locus_tag="VGP27_13655"
     CDS             2721..2975
                     /locus_tag="VGP27_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852378.1"
                     /translation="MTGREDPEAMDRIEQLPVSDWTDQDLLTKDEARERLVHEIGRTR
                     ARLDQLRAANADDPEIMLLERRLNAMESVRDEYTDYRDGK"
     gene            2977..>3473
                     /locus_tag="VGP27_13660"
     CDS             2977..>3473
                     /locus_tag="VGP27_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852379.1"
                     /translation="MSVAAEGAEDTAALRSHIRTFLENAPRPADLRNYGPTPTSADVE
                     PGRAWHKYLAEHGYVCLHWPAEYGGADASVAYQAIFAEECARAGVPRQLGITGVDLVG
                     PVLIKFGTDEQKSRYLEPIRLGDDIWTQLFSEPGAGSDLAGVRTKAERTPTGWRINGQ
                     KVWSSA"
BASE COUNT          626 a         1093 c         1175 g          579 t
ORIGIN      
        1 ggggcggcca gatgagctgc gcggggagat ggtgtgcgcg gtcgtgcggc ggtccgcacg
       61 acatcgcgac gtcacgctcc acgaactgtg cgcatttctc gacgagcgcg gcctgatgaa
      121 acagaaatgg ccggagcgac tgcactttgt cgacgagttc ccgctgaccg gcctcggcaa
      181 ggtcgcgaaa gccgaattgg cgcaactcat ttcgggaggc agccaatgac ggcggacgcg
      241 aggagcggaa agaataccgc ggacgcgagg agcacgacga ttacggtgtt gtccgtcgac
      301 gaacgcgacg agcttgcgcg ctcggtgcgt gccgcgtgcg agaagttcgc gtcgcaagag
      361 cgcgtccgtg cggtggccta tgacgatccc gaggacgcgc ggggcggctt cgacagcggg
      421 ctgtgggagg tgctctgcag ccaggtgggg gtggccgcca tcggcctccc ggagcacctt
      481 ggcggagcag gctacggcgc atccgcgttg ggagtggtcg cccacgaact gggccgcgtc
      541 ctggcgccgg tgccgttcgt ggcgtcgaca gtgctcgcca ccggcctcct gctggacctg
      601 cagggtcccg acggtggtgc ggaggagcgc ttctccgggc tgctcgaagg tcgacgcacc
      661 gccgcggccg cgatcaccgg cgacggcggg ctgtggcgtc gcaccgcggt gaccctgacc
      721 gccaccgagg gccacgacga ctcctggacc gtcgacggcg ttgtccgcca tgtgctcaac
      781 ggcacgaccg ccgacgatct cgtcgtggtg gcgacgacca acggcgaact ggccttgttc
      841 cttatcgacc cggccacaga cggcgttgaa gtcgatgcgg agcgcgttct ggacggcacg
      901 cggccgatgg cgaccctcgc cttcaccggc gtgcccgcgc tgaagctgag cagcgacggc
      961 cccgtcgacg acatcatcga gcgcaacgtc gacctggcgc tggcggtgct gtcggccgag
     1021 caggtcggcg cgtgtgagcg ggtgttggag attgccaccg aatacgcccg cacccgtgtg
     1081 caattcgacc gcgcgatagg cagcttccag gccgtcaagc acaggtgcgc cgacatgctg
     1141 gtcaatctgg agtgggcacg gtcggcgtcg gcggcggcgt tggagtccgt ggacgctgat
     1201 gcgaaagaga tggcaggcga atccgactgg cgcgccagca tggccaaagc ggtgtgctcg
     1261 gaagcgttgc gcgacgcggc gcacgccaat gtgcagatcc atggcggcat cggtttcaca
     1321 tgggaagact ccgcgcatct ttatctccgc cgggcccgca ccgacgaagt gttgttcggc
     1381 ggccctggac agcactggga ccggctcgcc atccggaaaa agctgctgtg accagcatgt
     1441 tccgccgtcc tcgacacccg cttgctggca catgggccgg tgactagaat cacttctaaa
     1501 taactaaatg tttttatgtt ttactcgatg atgaccgcag ccatatgagg aggcacctgt
     1561 gccgttgcag ccgtacatgg aattgatttc cgttgacgat catctgatcg aacaccccaa
     1621 ggtgtggagc gaccggcttc ccaagaagta cctggaggcc gggccgaaga tcatcgagtg
     1681 ggagcgtccg gacaccggtc agatgtgtca ggtgtgggac tacgagggcc gcatctaccc
     1741 ctacatcggt ctgaatgcgg tgacgggcaa gaaacccgag gagtacggaa tcgagcctgt
     1801 ccgttacgac gacatgattc cgggctgcta tgacccgaag gcccgcgtcg ccgacatgga
     1861 catcgacggt gtgcaggcga tgacgtgttt cccatcgttc ccccgtttcg ccggtgccgt
     1921 cttcgcggag ggcgaggaca aggaactggc caaactgtgt tcggcggcgt ggaacgactt
     1981 ccatctcgac gagtgggcgg ctaccgcacc ggaccgcttc atccccgttg cgatgctgcc
     2041 gttctgggat ctggatgcca gcgtcaagga gattcaccgg gtcgccgcca agggcgccaa
     2101 gtgcgtcacc ttccccgacc tgccggacaa gctgggcctg ccgtcggtgc actcggatca
     2161 ctgggatccg ctgctctcgg cgatggagga gacgggactc gttctggccc aacacttcgg
     2221 ctccggcgga ttcccaccgc cgatcgcccc ggacgccccg ttcgcggtgt tcgtcacgct
     2281 gatgggcacg atgtcgatga ccgcgcttac cgactggttg ttctcgcaca cgctctaccg
     2341 acatcccgac ctcaagatcg ggttgtccga gggcggcatc ggctggatcc cctacatcct
     2401 cgaacgggcc gacagcgtgt ggcgcaagca ccggttctac aacaacgtca atcaggacca
     2461 cgagccgtca tatctgtgga agaagcactt ccacggctgt ttcatcgaag acgacttcgg
     2521 cgtggccgtg cggcacgcgg tcggcgtgga ccggatcacc tgggaatgcg actatccgca
     2581 ttccgactcg ttctggccga acagccgcga ccgtgccgcg gaagcgttga aggatgtgcc
     2641 cgatgacgag gtgcatcaaa tggtcgagct gaacgcccgc gggttctaca acttcccgcg
     2701 agcggcgtaa cggtgggcag gtgacggggc gagaagaccc agaggctatg gacaggatcg
     2761 agcagcttcc agtttcggac tggacggacc aggatttgtt gaccaaggac gaggcgcgag
     2821 agcgtctcgt ccacgaaatc ggtcgcaccc gggcccgact ggatcagttg agggcggcga
     2881 acgccgacga tccggagatc atgctgctgg agcggcggct caacgcgatg gagtccgtcc
     2941 gcgacgagta caccgactac cgcgacggaa agtgatgtgt cggtagccgc cgagggcgcc
     3001 gaagacaccg ccgcacttcg gtcgcatatc cgcacgttcc tggagaacgc gcccagaccg
     3061 gcggatctgc gtaactacgg gccgacgccg acgtccgccg acgtcgagcc cggccgggcc
     3121 tggcacaaat acctcgccga acacgggtac gtctgcctgc actggcccgc ggagtacgga
     3181 ggtgccgacg cgtcggttgc ctaccaggcg atcttcgccg aagagtgcgc acgcgccggc
     3241 gtcccgcgcc agctcggcat caccggcgtc gatctggtcg ggcccgtcct gatcaaattc
     3301 ggcaccgacg aacagaagtc gcgatacctg gaacccattc ggctcgggga cgacatctgg
     3361 acgcagttgt tctccgaacc gggcgccggc tccgacctcg cgggcgtccg caccaaggca
     3421 gaacggacgc cgaccggctg gcggatcaac ggtcagaaag tctggagttc cgc
//
LOCUS       DASYLX010000568         8500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2002702,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000568 DASYLX010000000
VERSION     DASYLX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002702"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGP27_13665"
     CDS             <1..255
                     /locus_tag="VGP27_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7852380.1"
                     /translation="DMQAKRMASLQSTNPADPDAPQYGLGIAKYGALYGHTGEVPGFN
                     TFAGTDPEHKVTIVVWVNLEPTADGRAAATLIAKDLIEQM"
     gene            complement(699..1067)
                     /locus_tag="VGP27_13670"
     CDS             complement(699..1067)
                     /locus_tag="VGP27_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7852381.1"
                     /translation="MNIALWTVQGLLAFVYLAAGGLKVGRPREQLVASGRLEWMKDNS
                     DAAVKAIGAVEILGALGLILPGLTGIAPILAPIAAAGLVVVQIGALRVHLTRNERQPL
                     PANAILLLLAAFVAIGRMLG"
     gene            1173..1535
                     /locus_tag="VGP27_13675"
     CDS             1173..1535
                     /locus_tag="VGP27_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852382.1"
                     /translation="MTHVTPKVVRIPEGGAVREVLNRIGDKWSLLVIGTLRGGPLRFG
                     ELEEAVAGISQRMLTLTLKHLVEDGLVSRTAYAEVPPRVEYELTELGRTLLPLVISLA
                     EWAMAHHHEINANRRSQI"
     gene            complement(1904..2545)
                     /locus_tag="VGP27_13680"
     CDS             complement(1904..2545)
                     /locus_tag="VGP27_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEV7852383.1"
                     /translation="MSTLGRDGPIAARAAVAAACGLAVAICIGVSLEWKYAPACGWIA
                     AAVVFLIWTWAVIATMSPARTGSHATREDPTRVATDMFILSASVASLLGVAYLFAAGH
                     SQGVQANIAALVGVGSVAAAWLMVHTIFTVRYALLYYTDSAGGINFNQSEPPAYADFA
                     YVAFTIGMTYQVSDTDLKSRAIRRTALRHGLLSYLLGAIILAATVNLVAGLGR"
     gene            2709..3995
                     /locus_tag="VGP27_13685"
     CDS             2709..3995
                     /locus_tag="VGP27_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852384.1"
                     /translation="MRTRRRDDDVDVSRYVAETADRLHVRLAAVSSGVQGLLEDDIPE
                     LRGDARVVELLGASIEGNVETLLHALRYDIPVERVEAPTAALEYARRLAQHGVPVNAL
                     VRAYRLGQRQVNEIVFAEVRAIDIPESLRYTVIEAITRTLFEYIDWISQQVVAVYEDE
                     RERWLENQNSLRAVRVREVLAANKAVDADASSTSIRYPLRWHHLAVIMWCLDEGTEGD
                     ELASLQRFVRELAQSAGADANPLFVAADRMCGYGWLPFRAEPTDAAARVCAHAQKQGN
                     GPSLAIGTIAGGVEGFRRSHREAQGARAVAMAGGRHERVVISASDPGLSIASLLGDDV
                     GYTREWVDDVLGDLAADSENDERLRETLRIFLGCGSSYKMAAEELAMHFNTVKYRVGR
                     AEVRRGRPIAEDRLAVELALLLCHWYGGAVLRQGWR"
     gene            4081..5307
                     /locus_tag="VGP27_13690"
     CDS             4081..5307
                     /locus_tag="VGP27_13690"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852385.1"
                     /translation="MQNNVVQQQVAKVATALYRRTDELAPTLAHAITREVRLYQTTKP
                     VAFEVIVGGCSANMQPIFTAIANDSAFDPTAAAELGIQRAGDGVPLTSLLEAYRVGFR
                     GVWNAVVAESAMHNHVNGEALGVLTAKVSAAQDIYTDAMATAYRAEQARRVLSEESER
                     SVLIDSVLRGRLFEQWSVWEAADYLRLPTEGPYVVVAAEIAETGMQALPEVESKLRSL
                     DVFSAWRILPDLQVGIVHVKTDEQLGSVLALVSRMATTRVGVSSCFDDLRETAQALRY
                     ARITLRGRPDPERHVTQFDGSIIATAAVSAPEVMVKLVTPTIECFAELAEHERDTLFD
                     TFRVWLENDGSLRVAGEVLFCHPNTVRYRLHRIEQRTGRSLSRPRDVAEFSLAMEVHR
                     RLMWQTQDDEHRLPVP"
     gene            complement(5261..5725)
                     /locus_tag="VGP27_13695"
     CDS             complement(5261..5725)
                     /locus_tag="VGP27_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEV7852386.1"
                     /translation="MSSVRTEVSRLVPRRLLKGTEIHHPVVIDEARKRNASFQLRVAD
                     QITAFAGSMSFVFIHAALFAVWMLFFERSPWPTLTLVVSLEAIFLSTFVMIGQNRQAA
                     FQQTKADHDFQEQELELKTNTELTREIHRLTAELHKRLMEPAADAHRPASAT"
     gene            complement(5739..6359)
                     /locus_tag="VGP27_13700"
     CDS             complement(5739..6359)
                     /locus_tag="VGP27_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7852387.1"
                     /translation="MTETRQTPTTRSGPFHPVLDDVVSTTKNWWLLLVAGIAWIVIAV
                     VILRFDYATVATIATLFGFFSFLAAANEVMVGAVSTTGWRILHWILAALFVVVGVIAF
                     IRPSDTFVGLAAVMSFYFVFRGCFDIAMAFAATRVPGWWVLLLVGLAELAIGFWAAGS
                     WNVSVTVLVSWVAAGALIHGVGQIASAFAVRKVGHSVSVLDGQHAS"
     gene            6566..8068
                     /locus_tag="VGP27_13705"
     CDS             6566..8068
                     /locus_tag="VGP27_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7852388.1"
                     /translation="MSNLALNLVASSARIPDRIAAITDESTMTYAELDAASARLATLL
                     DREGIGAGDRVGVMLPNIAAAPITCYGIWRLGAVVVPMNPLMQGREVQFYLSNTDSKV
                     LIGSSGFAGAATEGADAAGAKLWLVDDAELSRLTVDLPEFGDPAERGDSETAVVLHTS
                     GTTGTPKGAELTHGSLGSNQNVIVRRLLKLTDEDVVLACLPLFHVFGMTCAMNAAIAA
                     GAGLSLIARFDPAKAIERIRRDRVTVLAAVPTMYSALLSVADQFSPSATASLRTCVSG
                     GAALPVAVLKAFEKTFRAVILEGYGLSETSPAVTFNHPGAHRRPGSIGTPIEGVQVRL
                     VDSDGVEVAPGTQGEIQIKGPNVMKGYWNLSDATDEVIKDGWFSTGDIAIADKDGYYY
                     VVDRKKDLIIRGGYNVYPREVEEALHEHPAVAAAAVIGIPHESLGEEIGAAVVLKVGA
                     SVEPDEVRQFVKGRVAAYKYPRRIWFVDALPTGPTGKLLRREIKPPPEEA"
     gene            8072..>8500
                     /locus_tag="VGP27_13710"
     CDS             8072..>8500
                     /locus_tag="VGP27_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) polymerase"
                     /protein_id="HEV7852389.1"
                     /translation="MVATTKAGADELAAPLDLLLTSSAVGMADRLMPNVAWSRFALNL
                     AKQPRTVASRAASLGRELVSIVEGRSELAPAKGDKRFADPAWRGNALLHRTMQAYLAT
                     NDTVNQLYSDADLDWRDGERIRFVLDLLTEALAPSNNPVLS"
BASE COUNT         1607 a         2612 c         2801 g         1480 t
ORIGIN      
        1 gacatgcaag ccaagcggat ggccagcctg caatcaacga atccggcaga ccccgatgcg
       61 ccacagtacg ggctgggcat cgcgaaatac ggtgcgttgt atggacatac cggagaggtt
      121 ccgggcttca ataccttcgc cggaaccgac cccgaacaca aagtgacgat cgtggtctgg
      181 gtcaacctcg agcccactgc ggatggtcgg gccgcggcca cgctcatcgc caaagacctg
      241 atcgaacaga tgtaaagtcc cgcgccgctc aacctgtggt cagggtcggc cgccttcgca
      301 gcacaccacg acgtatcgtg ccactaccga gccggttatt gggcttcgaa catcgcggca
      361 cccgagccct aacctgttcg gcggtagcaa cactgagcca ttttcacgtc gcgccgagcc
      421 gcttgtcgta gtcctggcgg ttgcagctgg gactccactc agtgggctag cgcggtgact
      481 cccgccgctc tgcgcccata tcagcccaga tgaggtaccc gccgaccggc ggacaccgga
      541 gcaccaccga tgaccgacgt acagccctac gcccaccctc gcggggcgta aggcgcaacc
      601 gttcggttac gcgggctgac cacttccgac ggtcctcgac ctcacagcgg tcaggctgtt
      661 catcgcgcaa tcagctcagc ggaggcattg cgagattttc agcccagcat gcggccgatc
      721 gcgacgaagg ctgcgagcag cagcaggatc gcgttggcgg gcaaaggttg ccgctcgttg
      781 cgggtcaggt gcacccgaag tgcgccgatc tgcacgacga ccagaccggc tgccgcgatc
      841 ggggccagaa tgggcgcgat gccggtcagt ccgggcagaa tcaagcctag tgcgccgagg
      901 atctcgaccg cgccgatcgc tttcactgcc gcgtcagagt tgtccttcat ccactcgagt
      961 cggcccgatg ccacgagctg ctcgcgcggc cgaccgacct tgagtccgcc cgcggcgaga
     1021 tagacgaagg cgagtaggcc ctgcaccgtc caaagtgcga tgttcaccgt gttcccccat
     1081 ccttactatt tgtgcgtagc cacatcgtgt gtgagcttgc gggcggcgac aaaaggcaca
     1141 tcgatgtatg tctccaggaa ggaggctccg gtatgacgca cgtcacaccg aaggtggtgc
     1201 ggatacccga aggaggggcg gtccgggagg tactgaaccg catcggcgac aagtggagtc
     1261 tgctggtcat tggcacgcta cgtggcgggc cgctgcggtt cggggagctg gaggaggccg
     1321 tagcgggcat ctcgcagcgc atgctcaccc tcacgctcaa gcacctggtc gaggacggcc
     1381 tggtcagtcg aacggcctat gcggaggtgc cgccgcgggt cgagtacgaa ttgactgagc
     1441 tggggcggac actgctaccg ctggtgatct ccctcgccga atgggccatg gcgcaccacc
     1501 acgagatcaa cgccaatcgc aggtcgcaga tctaggtgcg gcgctccgtg aagacgagat
     1561 gacggcggag ccgcacgcct gcgccggggg ccggaatacc agggactacg tcgaacactt
     1621 cccacttctg ggtgtacgcg ggttgcacac ggcaggatga cccaaagtcg ccgcggaatg
     1681 ttctgggagg agtcacctga cggggcattc ctgggagttc tcaggcgaag cgcggtgcga
     1741 gatccagcag ccgccgcagc agcgcgccac taccgatccg gttgtcggct tcgaacatcc
     1801 gcgtcgcctg agccctaacc tgtttcgcgg tagaagattt gagccatttc acgtcctgcc
     1861 gagccgcttg tcctagtcct ggaagttgca gctgcgacgc cactcagcgg cctaggccgg
     1921 cgaccaagtt caccgttgcc gcgaggatga tggccccgag gagataggac agcagaccgt
     1981 ggcgtaaggc cgtccttcga atcgcgcggg atttgagatc ggtgtctgac acctgatagg
     2041 tcatgccgat cgtgaaggct acgtatgcga agtcggcgta cgccggcggt tcggattgat
     2101 tgaaattgat gccgcccgct gaatcggtgt aataaagcaa cgcatagcgc acggtgaaga
     2161 tcgtatgcac cattagccac gccgccgcga cgctgccgac acccaccagg gcggcgatat
     2221 tggcttgcac tccctgtgag tggcccgcgg cgaagaggta ggcgaccccg agcaggctcg
     2281 ccacgctagc gctaagaatg aacatgtcgg tggccacccg ggtcggatct tcgcgggtgg
     2341 cgtgagatcc cgtccgtgcc gggctcatgg tcgcaatcac cgcccacgtc cagataagga
     2401 acacgactgc agcggcgatc cagccgcacg ccggcgcata cttccactcc agggatacgc
     2461 cgatgcagat cgccacggcg aggccgcacg ctgccgccac ggcagcacgc gccgcgatgg
     2521 ggccgtcgcg gcccagcgtg ctcacgtagc cgagtgtcgc atcgtctcgt ctcatccaac
     2581 ctgcgacacg acaagtgggc gtcatggacg tggtgtgatt cgtcgggcgg cttcggcgcg
     2641 gcgccacagc ttgcccgtgg gcctgcaaat cgccggttat cggcggcccg acgatctact
     2701 gtcaagaggt gcgcacccgg cgtcgcgacg atgacgtcga cgtgagtcgc tacgtcgccg
     2761 agaccgccga ccggctgcat gtccggctgg cggcggtgtc gtctggggtt cagggtctgc
     2821 tggaggacga tatccccgag ctgcggggcg atgcgcgggt cgtggagctg ctcggcgcca
     2881 gcattgaggg aaacgtcgaa acccttctgc acgcactgcg ctacgacatc ccggtggaga
     2941 gggtggaggc cccaacggcg gcgctggagt atgcccgccg gctcgcccaa cacggcgtcc
     3001 cggtgaacgc gctggtccgt gcctaccgtc tcggtcagcg ccaggtgaac gagatcgttt
     3061 tcgccgaggt ccgggcaatc gatatccctg aatcgctgag gtacaccgtc atcgaggcga
     3121 tcacccgcac cctgttcgaa tacatcgact ggatctcgca gcaggtcgtc gcggtgtatg
     3181 aagacgagcg ggaacgctgg ctggagaatc agaacagcct tcgagccgtg cgagttcgcg
     3241 aggttctggc cgcgaataag gccgtcgacg cggatgcgtc gagcacatcg atccgctatc
     3301 cactgcgatg gcaccatctg gcggtgatca tgtggtgcct tgacgagggc accgaaggcg
     3361 acgaacttgc ctcgctgcag cggtttgtcc gggaactcgc gcaatccgca ggggccgacg
     3421 caaacccatt gttcgtcgcg gccgaccgga tgtgcgggta tggatggctg ccgtttcgtg
     3481 cggaaccgac ggacgcagcc gccagagtgt gcgcccacgc ccagaagcag gggaatggcc
     3541 ccagtttggc gatcgggacg atagcgggcg gcgtcgaggg attccgccgg tcgcaccgcg
     3601 aagcacaagg agcgcgcgcc gtcgcgatgg cgggtggccg ccacgaacgc gtggtgattt
     3661 ccgccagcga ccccggcctg tccatcgctt cgctcctcgg tgacgatgtg ggctacaccc
     3721 gcgaatgggt tgacgacgtg ctcggggacc tggccgccga tagcgagaat gacgagcgat
     3781 tgcgtgagac gctgcggata ttcctgggct gcggcagcag ctacaaaatg gcggccgagg
     3841 aactcgccat gcacttcaac acggtgaagt accgcgtcgg gcgcgccgaa gtccggcgcg
     3901 gtcggccgat cgcagaggat cggctcgctg ttgagctcgc gttgttgttg tgccattggt
     3961 atggcggcgc cgtcctgagg cagggctggc gatagcagga tcgccggaga caacggcaac
     4021 ggcgaacttc gtcctccggg aacaatcgcc acggggcggc tgagcctaat ctttccgcgc
     4081 atgcagaaca atgtggtcca acaacaggtc gcgaaggtcg caaccgcgct ttaccggcga
     4141 accgatgagc tcgcgcccac gcttgcccat gcgatcactc gggaagtgcg gctctaccag
     4201 acgacgaagc cggtggcctt cgaggtcatc gtcggcggat gctcagcgaa catgcagccg
     4261 atcttcacgg cgatcgcgaa cgattcggcg ttcgacccaa ccgccgcggc ggagctgggt
     4321 attcagcgtg ccggagatgg ggtgccgctc acgtcgttgc tggaggcata ccgggtcgga
     4381 ttccgtggcg tgtggaacgc ggtggtggcg gagtccgcga tgcacaacca tgtcaacggc
     4441 gaagccttgg gcgtgctgac cgcgaaagta tctgccgccc aggatattta caccgacgcg
     4501 atggccaccg cataccgggc cgagcaggcg cgccgggtgc tcagcgagga gtcagagcgc
     4561 tccgttctca tcgactcggt gctgcgcgga cggctgttcg aacaatggag cgtttgggaa
     4621 gcggccgact acctccggct gcctaccgag ggaccttatg tcgtcgtcgc cgccgaaatc
     4681 gccgagactg ggatgcaggc tctgccggaa gtcgaatcga agctgcgcag cttggatgtg
     4741 ttctccgcct ggcggattct gccggacttg caggttggga tcgtgcatgt caagactgac
     4801 gaacagctgg ggagtgtgct ggcgctcgtg tcccggatgg cgacgacgcg ggtaggggtg
     4861 agctcctgct tcgacgacct gcgcgagacg gcacaagcgc ttcgatacgc caggattacc
     4921 ctgcggggca ggccggaccc ggagcggcat gtcacacagt tcgacggctc gataatcgcc
     4981 actgccgcag tcagcgcccc agaggtgatg gtcaaactgg tgacgccaac gatcgagtgc
     5041 ttcgccgaac tggctgagca tgagcgtgac actctcttcg acacattcag ggtgtggttg
     5101 gagaacgacg gatcgctccg ggtggcaggc gaagtgctgt tctgccaccc caacaccgtg
     5161 cgataccggt tgcatcgcat tgagcaacgt actggccggt cactgtcacg gccgcgcgat
     5221 gtcgccgaat tctccctggc gatggaagtt catcgccgtc tcatgtggca gacgcaggac
     5281 gatgagcatc ggctgccggt tccatgaggc gtttgtgcag ttcggctgtg agtctgtgga
     5341 tttcccgagt gagctcggtg ttggtcttga gttccagttc ctgctcctgg aagtcgtgat
     5401 cggccttagt ttgttggaac gccgcctgtc ggttctggcc gatcatcacg aaggtcgaca
     5461 agaagattgc ctcgagcgag accaccaggg tcaatgtcgg ccacggactg cgttcaaaaa
     5521 ataacatcca caccgcgaac aacgccgcat ggatgaagac gaagctcatg gatccggcga
     5581 atgccgtgat ctgatcagcc actcgcagtt gaaaactcgc gttgcgtttg cgcgcctcgt
     5641 cgatcaccac cgggtggtgt atttcggtgc ccttcagaag ccgacgcggt accagccggc
     5701 tgacttcggt gcgtactgaa ctcatcacac cgggcgattc acgacgcgtg ctgtccgtcg
     5761 aggacagaaa cgctatggcc gaccttgcgc acggcgaagg cggaggcgat ctgaccgaca
     5821 ccgtgaatca gcgcgccggc agcgacccac gacaccagca cggtcaccga cacgttccac
     5881 gagccggcgg cccagaaacc gatcgccaac tccgccaggc cgacgagtag tagtacccac
     5941 cacccgggca ccctggtcgc cgcgaatgcc attgcgatgt cgaagcatcc acggaaaacg
     6001 aaatagaagc tcatgacggc ggcaaggccg acgaacgtgt cgctgggccg gatgaacgca
     6061 atgacaccga ccacgacgaa cagcgccgca agaatccagt gcaggatgcg ccaaccggtg
     6121 gttgagacgg cgccgaccat tacttcattc gctgctgcca ggaaactgaa gaatccgaaa
     6181 agagtggcga tcgtcgcaac cgtggcgtag tcgaatcgca ggatcaccac tgcgatcacg
     6241 atccacgcaa tgccggcgac gagtagtaac caccagttct tcgtggtgga tacgacgtca
     6301 tcgaggacgg gatgaaacgg acctgatcgt gtcgtcgggg tctgacgggt ttcggtcatt
     6361 tccactccca agtgattccg gtgtcatcga ttcacgcggg gacacccgcg cgggccagcg
     6421 gtattcattg ttgctccgtc tgcagactgt tcgattgttt gtggttacaa catcaccggg
     6481 gctgcggtgt tcggcgcaac aacgacaggg cggcgtgcag tcgagacgat tgagctacca
     6541 cccaagcggg acagatggag ccgcaatgtc gaatctcgct ctcaatctcg ttgctagttc
     6601 cgcgcgtatc cccgatagga tcgcggcgat caccgacgaa agcacgatga cgtacgcgga
     6661 actggacgcc gcgtcggcgc ggctggcaac tctgctcgac cgcgagggga taggcgcggg
     6721 agatcgggtc ggcgtgatgc tgccgaacat cgccgcagca ccgatcacgt gctacggcat
     6781 ctggcggctc ggtgccgtcg tggtgccgat gaatccgctc atgcagggcc gcgaagtgca
     6841 attttacctg tcgaacaccg actcaaaggt gctgatcggg agctcagggt tcgccggcgc
     6901 tgcgaccgag ggtgccgacg ccgcaggcgc gaagctgtgg ctcgtcgatg acgccgaact
     6961 gtcccggttg accgtcgact tgcccgagtt cggtgatccc gccgaacgtg gcgactcaga
     7021 gactgcggtg gtgctgcaca cgtcgggaac caccggcacg ccgaagggcg ccgaactgac
     7081 gcacggaagc cttggctcga accagaacgt catcgtgcga cgcctgttga agctgaccga
     7141 tgaggacgtc gtgctggctt gcttgcccct gttccatgtg ttcgggatga cgtgcgcaat
     7201 gaacgccgcg atcgccgcag gcgctggact ttcactgatc gcgcgctttg acccggccaa
     7261 ggcgatcgag cgaatccggc gcgaccgggt aacagtgctg gcggccgtgc caaccatgta
     7321 cagcgcgctg ctgtcggtgg ccgatcagtt ctcgccatcg gcgacggcct cactgcgcac
     7381 gtgcgtgtcc ggcggcgcgg ccctgcccgt cgcggttctc aaggctttcg agaagacctt
     7441 ccgcgcggtg atcctggagg gctacggcct ctccgagaca tcgccggccg tgacgttcaa
     7501 tcaccctggc gcgcaccgcc gcccgggctc gattggcacc ccgatcgagg gagttcaggt
     7561 gcgccttgtc gattcggacg gcgttgaggt ggcgcccggc acgcagggcg agattcagat
     7621 caagggtcct aacgtgatga aggggtattg gaatctgtcc gatgccaccg acgaggtgat
     7681 caaggacggc tggttttcca cgggcgatat cgctattgca gacaaggacg ggtactacta
     7741 cgtcgtcgac cggaagaaag acctgatcat tcgtggcggc tacaacgtgt acccacgaga
     7801 ggtcgaagag gcgctgcacg agcatccggc cgtggccgcg gctgcggtca tcggcattcc
     7861 ccacgagtca ctgggcgagg aaatcggcgc tgcggtggtt ctgaaggtag gcgcgtcggt
     7921 cgaaccggac gaagtgcggc aattcgtcaa gggccgggtc gcggcgtaca agtacccgcg
     7981 ccgcatttgg ttcgtcgacg cgctcccgac cggcccgacg ggcaagcttt tgcgccgtga
     8041 aattaaaccg ccacccgagg aggcgtaaca cgtggtagcc accacgaaag cgggtgccga
     8101 cgagcttgcg gcaccgctgg atctgctgtt gacgagttcg gctgtcggca tggcggatcg
     8161 gctgatgccc aacgtggcgt ggagccgatt tgcgctcaac ctcgccaagc agccccgcac
     8221 agtggcgtcg cgcgcagcgt cgctcggtcg cgaattggtg tcgattgtcg aaggccgctc
     8281 ggagctggcg cccgcgaagg gggacaagag attcgccgac ccggcatggc gcggcaatgc
     8341 gcttcttcac cgcacgatgc aggcctacct ggcgacgaac gacactgtca accaactgta
     8401 ttcagatgcg gacctcgact ggcgggatgg cgagcggatt cgattcgtgt tggatctcct
     8461 caccgaggcg ctcgcaccga gcaacaaccc ggtgctgagc
//
LOCUS       DASYLX010000569         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_247728,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000569 DASYLX010000000
VERSION     DASYLX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247728"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VGP27_13715"
     CDS             complement(<1..804)
                     /locus_tag="VGP27_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEV7852390.1"
                     /translation="MRLALRAATLYYLDGLTQAEIAVRLGVSRPTAGRLIARAKANGL
                     VRIDVVVPPDMRDDLHAEEERELEHRFGLTEAVVTGHGIDVGAPGRPAAFASVGRAAA
                     ALVMRRLTPNDVLGFTWGPEQVAVVTAMAPGVASCRAVVQLDGAMSSAAYQTGTEFIL
                     SRCADVLRATTIRLPAPLYADPSTVASMRSDSLISRTLEAGRRADVMLFGVGAVSTST
                     TLFEGSFLDTRMLDELIKLGAVGEIGGRFFDGHGAPVETELQLRAVSVPL"
     gene            1012..2010
                     /locus_tag="VGP27_13720"
     CDS             1012..2010
                     /locus_tag="VGP27_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7852391.1"
                     /translation="MRVAVLIEPGRIEMEQRPVPRPAPGDVLIRVSSVGVCGSDTHYY
                     RHGRIGSFVVDGPLVLGHEAAGTIVGVGESVDPSRIGQRVSIEPQRPDPESAETRRGD
                     YNLCPHMRFFGTPPVDGALCDYVTIGATFAHPVPDTVSDDAAALCEPLSVGIAALRKA
                     GIGKGSRVLVAGAGPIGIVVAQLARAYGATDIAVSDPDGSRRNQARANGATVTLDPAA
                     DELGGGFDAFIDASGAPAAVTSGIRALRPAGTAVLVGSGAESMDLPTQLIQNRELTLT
                     GVFRYANTWPEAIALVESRRVDLDSMVTARFPLERTAQALDSDRMPGSVKTVVAVS"
     gene            2007..3431
                     /locus_tag="VGP27_13725"
     CDS             2007..3431
                     /locus_tag="VGP27_13725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEV7852392.1"
                     /translation="MKLSSSTLAQIQISKPTYNRDEIAIGIVHFGVGGFHRAHQAMYI
                     DRLLEKGFANDWGICGIGVMPADRKMKEVLDAQDGLYTLVLENPDGTRDARVIGSIVD
                     YLYAPDDPEAVIELLAAPTTRIISLTITEGGYNVENVKPDEVSVFGLVAEALARRRDR
                     GVASPTIVSCDNIEGNGEVARQAFTAYAERVHRGLAQWISTNTRFPNSMVDRITPATT
                     PEVIDNLKREFGVDDQWPVVAEPFTSWVLEDDFSYGRPPLEDVDVLLVDNVRPYELMK
                     LRLLNASHQSLCYFAYLSGYRLVHDAAGDPLFADFLMRYMDAEATPTLLPVPGIDLPD
                     YKRTLIQRFANPGVRDTVARLCFGSSDRIPKWLLPVVRANLASDGPIELSAATVASWA
                     RYDEGVDEQGEPIDIQDQLADTLVALARAQRDNPTAFIENTDVFGDLATSGRFVEAYT
                     WALESLHRDGARATLEALMQKDPA"
     gene            3428..4384
                     /locus_tag="VGP27_13730"
     CDS             3428..4384
                     /locus_tag="VGP27_13730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV7852393.1"
                     /translation="MSPQRALVIGEALIDIVERDGQIMGEHVGGSPLNVAVGLARLGR
                     GVDFLTHIGDDDRGRRIAGYVKSSGAELVSGSITADHTPTAVATLDESGQAQYVFDID
                     WQLAGTPEVTPPLVAHTGSIATVLEPGCRATAALLNAYHVSATISFDPNVRPALIEDD
                     EQARTRIDRLVERCDVVKASDEDVDWIDPNRTPEQVARTWLGLGPAIVAVTMGERGAF
                     AICQAGVARVASRPVNVVDTVGAGDAFMTGLIDALWSLGLLGAERRADLRRISVDTLT
                     EVLQTAALSSALTVARAGADLPDRATRDAAAASADKSRTHGG"
     gene            4407..5366
                     /locus_tag="VGP27_13735"
     CDS             4407..5366
                     /locus_tag="VGP27_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV7852394.1"
                     /translation="MRSIWKGSISFGLVNVPVKVYSATQDHDVKFHQVHAKDNGRIRY
                     KRVCELDGEVVEYRDIARAYETDDGHMVVITDEDISTLPEERSREIEVVEFVPASEID
                     PLMYDKSYYLEPDGKSSKSYVLLAKTLAETDRVAIVHFALRNKTRLAALRVKDFSKRD
                     IMVIHTLLWPDEIRDPDFPVLDKEVEIKPAELKMAGQVVESMTDDFHPERFEDTYQEQ
                     LKELVEAKLEGGEAFTAEEAEPKELDDTEDVSDLLAKLEASVKARRGAGSASDDGQAK
                     KTSAKKTPAKKSAAKKAPAKKTAAKAAKKAPAKKAAKKAAAKK"
BASE COUNT         1021 a         1810 c         1862 g          866 t
ORIGIN      
        1 gagcggaacc gacaccgcgc gcagctgcag ctcggtctcg accggtgcgc cgtgaccgtc
       61 gaagaaccgc ccgccgatct cgcctaccgc accaagcttg atgagctcgt cgagcatccg
      121 ggtgtcgagg aagctgccct cgaacaaagt cgtcgaggtg gaaacggcgc cgacgccgaa
      181 caacatcacg tctgcgcgcc tgcctgcctc cagagtgcgg gatatcagcg agtcgctacg
      241 catggaggcc acggtggacg gatcggcata cagcggcgcg ggcaagcgga tcgtcgttgc
      301 ccgcaataca tcggcacagc ggctcaggat gaactcggta ccggtctgat acgcggcgct
      361 ggacatggct ccgtccagct ggaccaccgc gcggcaactt gccactccgg gtgccatggc
      421 ggtgactacc gcaacctgtt cggggcccca cgtgaatccg aggacgtcgt tgggggtcag
      481 ccggcgcatc acgagtgccg cggccgcgcg gccaacgctg gcaaaagccg ccggtcgtcc
      541 cggcgccccg acgtcgatgc cgtgacccgt gaccaccgct tcggtgaggc cgaagcggtg
      601 ctcgagttct cgctcctcct cggcgtgcag atcgtcgcgc atgtccggcg ggacgacgac
      661 gtcgatacgg acgagcccgt tggccttggc ccgcgcgatc agccgtccgg ccgtcggtcg
      721 ggacaccccg agccgaaccg cgatctcagc ctgggtgagc ccgtcgaggt agtagagggt
      781 cgcggcgcgc aaggccaatc gcaggtcttc ggggctggcg ctgcggtcct tgggcgagtg
      841 aagtccctgg ccatcgaccg tcccggcgac ctcgccgatt gacgtggatg cgaccacggg
      901 cctacctcca ctttccggca ccccgtgagc agatgcttag gggtgcgagt agatgttcat
      961 cacgctagca tggtcagtgt gacgtacaca acactcgcgc cggacaacca aatgcgggtt
     1021 gccgtcctaa tcgagccagg tcgaatcgag atggagcagc gtcccgtgcc gagaccggcg
     1081 ccaggtgatg tgttgatccg ggtgtcgtcg gtcggggtct gtgggtccga cacgcactat
     1141 taccgccacg gccggatcgg cagcttcgtc gtcgacggcc cgttggtact cggccacgaa
     1201 gcggcgggga cgattgtcgg cgtcggcgag tcggtcgacc cgtcgcgcat cggtcagcgc
     1261 gtgtcgatcg aacctcagcg ccccgacccg gaaagtgcgg agacccgccg tggcgactac
     1321 aacctctgcc cgcacatgcg gttcttcggt accccgccgg tcgacggggc actgtgtgat
     1381 tacgtgacca tcggtgcgac gttcgcacac ccggtgccgg acaccgtctc cgatgacgcc
     1441 gccgcgttgt gcgaaccgct gtcggtcggc atcgccgcgc tgcgcaaagc gggcatcggc
     1501 aagggatcgc gggttctcgt cgccggtgca ggacctatcg gcatcgtggt ggctcagctc
     1561 gcacgcgcct acggcgcaac cgacatcgcc gtttccgatc ccgacgggtc gcgacgcaac
     1621 caggcacggg caaacggcgc caccgtcacc ctcgaccctg cggccgacga gctgggcggt
     1681 ggtttcgacg ctttcatcga cgcctccggc gcgcccgctg cggtgaccag tggcatccgt
     1741 gcactccggc ccgcggggac ggcggtgctg gtcggctcgg gagctgaatc gatggacctg
     1801 ccgacacagc tgattcagaa ccgcgaactc accctgaccg gcgtcttccg ctacgccaac
     1861 acctggccgg aggcaattgc cctcgtggag tcacgccggg tcgatctgga ctccatggtc
     1921 actgcgcggt tcccgctcga gaggaccgca caagcactgg actccgaccg catgccgggt
     1981 agcgtcaaga cggtggtggc ggtgtcatga agctgagcag ttcgacgttg gcccagatcc
     2041 agatcagcaa gccgacttac aatcgcgatg aaatcgctat cggcatagtg catttcgggg
     2101 tcggcggctt ccaccgtgcg catcaggcga tgtacatcga ccgactgctg gagaagggct
     2161 tcgcaaacga ttggggcatc tgcggcatcg gcgtcatgcc ggccgaccgc aagatgaagg
     2221 aggtcctcga cgcgcaggac ggtctgtaca cgctggtgct cgagaacccc gacggcactc
     2281 gcgacgcgcg ggttatcggg tcgatcgtcg attacctcta tgcacccgac gacccggaag
     2341 ccgtgatcga actgctggcc gcgccgacga cgcgcatcat ctcgctgacc atcaccgagg
     2401 gcggttacaa cgtcgagaac gtcaaacccg acgaggtcag cgtgttcggt ctggtggccg
     2461 aggccctcgc gcggcgccgc gaccgcggag tggcgtcccc gacgatcgtt tcgtgcgaca
     2521 acatcgaggg caacggcgag gtcgcacggc aggcgttcac cgcctacgcc gagcgcgttc
     2581 atcgcggcct ggcgcagtgg atctcgacca acacacgttt ccccaactcg atggtggacc
     2641 gcatcacgcc ggccaccacg ccagaggtca tcgacaatct gaagcgcgag ttcggcgtgg
     2701 acgaccagtg gccggtagtg gccgagccgt tcacctcgtg ggtgctcgag gacgacttct
     2761 cctacggcag gccaccgctg gaggacgtcg atgtgctgct ggtcgacaac gtgcggccgt
     2821 acgagctgat gaagctgcgg ctgctcaacg ccagccacca aagcctctgc tacttcgcgt
     2881 atctcagcgg ctaccgactg gtgcatgacg ccgcgggcga tccgctgttc gccgacttcc
     2941 tgatgcgata catggatgcg gaggcgacgc cgacgctgct gccggttccg ggaatagatc
     3001 tgcccgacta caaaaggacc ctgatccagc ggttcgcgaa ccccggcgtt cgcgataccg
     3061 tcgcccggct gtgcttcggc tcgtcggacc gcatcccgaa gtggctgctt cccgtggtcc
     3121 gcgcgaacct ggcctccgac ggcccgatcg agttgtcggc ggcgacggtg gccagttggg
     3181 cccgctacga cgagggcgtc gatgagcagg gcgaaccgat agacatccag gatcagctgg
     3241 cggacacgct cgtcgcgctg gcccgcgctc aacgcgacaa cccgacggca ttcatcgaaa
     3301 acaccgatgt gttcggggat ctcgccacct ccggccgatt cgtcgaggcg tatacgtggg
     3361 cactggagtc gttgcaccgc gacggcgcga gggcgacgtt ggaggccctg atgcaaaagg
     3421 atccggcatg agcccgcaac gggcgctggt catcggcgag gctctgatcg atatcgtcga
     3481 gcgggacggc caaatcatgg gcgagcacgt cggcggaagc ccgctcaacg tcgccgtcgg
     3541 cctcgcgcgg ctcggccgtg gtgtcgactt cctcacccac atcggtgacg acgatcgcgg
     3601 ccgccgcatc gccggctacg tcaaaagctc tggcgccgaa ttggtttcgg gaagtatcac
     3661 cgccgaccac actcccaccg cggtggccac gctggacgag agcggacagg cgcagtacgt
     3721 cttcgacatc gattggcagc tggcaggcac accggaagtg accccgccgc tcgtcgcgca
     3781 caccggatcc atcgccacgg tcctcgagcc cggctgccgc gcgaccgccg cgctgctgaa
     3841 cgcctaccac gtctcggcca cgatcagctt cgatccgaac gtgcgaccgg cgctgatcga
     3901 ggacgacgag caggcgcgca cccgtatcga ccgcctcgtc gagcggtgcg atgtggtcaa
     3961 ggccagcgac gaggacgtgg actggatcga cccgaaccgg acacccgagc aggtggcgcg
     4021 gacgtggctc ggtctggggc cggcgatcgt cgcggtgacg atgggtgagc gcggagcttt
     4081 cgcgatctgc caggcggggg tcgcgcgcgt cgcgtcgcga ccggtgaacg tggtggacac
     4141 cgtgggcgcg ggcgacgctt tcatgaccgg cctgatcgac gcgctgtggt cgctgggcct
     4201 gctcggcgcg gagcgacgcg ccgacctgcg ccggatcagc gtcgacacgc tgacggaagt
     4261 actgcagacc gccgctctgt cgtcagcgtt gacggttgca cgggcgggcg ctgacctgcc
     4321 ggatcgagcc accagggatg ccgccgcggc aagcgccgac aagtcgcgga cgcatggcgg
     4381 ctaactgatt ggggaatact agggctatgc ggtccatatg gaagggctcg atctccttcg
     4441 gactggtcaa tgtccccgtc aaggtctaca gcgccacgca agaccatgac gtgaagttcc
     4501 accaggtcca cgcgaaggac aacggacgca tccgttacaa gcgcgtctgc gagctggacg
     4561 gggaagtcgt cgaataccgc gacatcgcgc gggcatacga gaccgacgat ggccacatgg
     4621 tggtgatcac cgacgaggac atctcgaccc tgccggagga acgcagtcgc gagatcgagg
     4681 tggtcgagtt cgtgccggcc agcgagatcg acccgttgat gtacgacaag agctattacc
     4741 tcgagcccga cgggaagtcg tcgaagtcct acgtcctgct ggccaagacg ctggccgaga
     4801 ccgaccgggt ggccatcgtg cacttcgcac tgcgcaacaa aacgcgtctc gcggcgttgc
     4861 gcgtcaagga cttcagcaag cgcgacatca tggtcatcca cacgctgctc tggcccgacg
     4921 agatccgcga ccccgacttc ccggtgctgg acaaggaggt cgagatcaaa ccggccgagt
     4981 tgaagatggc cggacaggtg gtggagtcga tgaccgacga cttccacccc gagcgcttcg
     5041 aggacaccta ccaggagcag ctcaaggagc tggtggaggc gaaactcgaa ggaggagagg
     5101 cgttcaccgc ggaagaggcc gaacccaagg aactcgacga caccgaggac gtctcggatc
     5161 tgctcgcgaa actcgaagcc agcgtcaagg cgcggcgtgg cgcgggcagc gcatcggatg
     5221 atggtcaggc caagaagacg tctgcgaaga agacgcccgc caagaagtct gcggccaaga
     5281 aggcacccgc gaagaagacc gccgcaaagg cggctaagaa ggcacccgcc aagaaggcgg
     5341 ccaagaaggc cgcagcgaag aagtaacgat cagtgagacg tcagcagccg agatggttga
     5401 tgctggcgac gacgatttcg taggccgcgg agatcgaaac gccgaggtcc ttgctgacct
     5461 tgatgacgtt gttcgaagca ggcacgccgc tgcgtgccgt cgcgcacacc tgatgacccg
     5521 tggacagcag ctgcgactcg ctcaagtacg cgtacctgg
//
LOCUS       DASYLX010000570         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2113109,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000570 DASYLX010000000
VERSION     DASYLX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113109"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGP27_13740"
     CDS             <1..649
                     /locus_tag="VGP27_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7852395.1"
                     /translation="RPAPLRQAVYEAIVELIVHGTLKRGQHLVENELAEYLGVSRQPV
                     REALQRLQTDGWIELRPAQGAFVHVPSEEEVDQLLSVRTVLEAYSARLAAERATPEDV
                     NLLWELQQDGLNALTANDPEGLVAANAALHATITALAGNAVLAEHISLVERKWRWYYL
                     PIAQPRGQGAWTEHAALIKAIAAGDADQATDIMTRHTELTRESYHEQRIAKTDTQ"
     gene            complement(656..1522)
                     /gene="fdhD"
                     /locus_tag="VGP27_13745"
     CDS             complement(656..1522)
                     /gene="fdhD"
                     /locus_tag="VGP27_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV7852396.1"
                     /translation="MGRVTSRYRVVRMADGVADYRPDTLAAEEPLEIRVAGRPLTVTM
                     RTPGDDFDLARGFLVSEGVVSAASDIAGIRYCAGATANGVNTYNVLDVLLEVGVAAPD
                     PSLERNFYTTSSCGLCGKASLEAVRTISKWHVARDPLRLNAATITTLPEKLRAAQRVF
                     DRTGGLHAAALFDADGELLCVREDVGRHNAVDKVIGWALDAGRLPLSGTTLMVSGRAS
                     FELVQKAVMAGVPALAAVSAPSSLAVDLAREMGLTLIGFLRGSSMNIYSGLERISADC
                     DESADTDRRQAI"
     gene            1608..>2258
                     /locus_tag="VGP27_13750"
     CDS             1608..>2258
                     /locus_tag="VGP27_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852397.1"
                     /translation="MSPTSAPVAVVEVLDLHGRSYRIGERPHDIIGHSRKWMLWLSWA
                     AMAGIGVLQYGYGVAITALHVTDGSTVTGALWVLALWVVFQAGAAAPTAALSHRFALA
                     PSRAVLVGALLCAAGPLTLAYTDSFALAVLGYSVLSGTGAGIVYATCTSTVAKWYPEQ
                     RAVRVGFVTGAFGYGAVPFIALFAAVLTPGNRAAIFAGVGVCVLLVVAVCGALLRDP"
BASE COUNT          379 a          780 c          717 g          382 t
ORIGIN      
        1 gcgtcccgcc cccctgcgtc aggccgtcta cgaagcgatc gttgaactga tcgtgcacgg
       61 aactctgaaa aggggccagc acctggtcga gaacgagctg gcggagtacc tcggcgtcag
      121 caggcagccg gtgcgagaag ccttgcagcg cctacagacc gatggctgga tcgaactacg
      181 gccggcacaa ggcgccttcg tgcacgtccc ttccgaggaa gaggtagacc agctcctcag
      241 cgtgcgaacc gtcctggagg catactcagc ccggctcgcc gccgagcgcg ccacccccga
      301 ggacgtcaac ctgttgtggg agttgcagca ggatggcctg aatgcgttga cggccaacga
      361 cccggaggga ctggtggcgg ccaacgcggc actgcacgcc acgatcactg ccctcgcggg
      421 gaacgccgtg ctcgccgagc acatctcact cgtcgaacga aagtggcgct ggtattacct
      481 cccgatcgcg caaccacgcg gccagggcgc gtggaccgaa cacgccgcac tgatcaaggc
      541 catcgcggcc ggcgacgccg atcaggcgac cgacatcatg actcggcaca cggagttgac
      601 tcgcgagtcc taccacgaac agcggattgc caagaccgac acccagtagc ggccctcaga
      661 tcgcctgtcg ccgatcggta tcggcacttt cgtcgcaatc ggcagagatg cgctcgagtc
      721 cggaatagat gttcatcgac gatccgcgca ggaaaccgat caacgtcagt cccatctccc
      781 tggcgaggtc gacggcaagc gacgacggcg cggacaccgc ggccagggcg ggcacgccgg
      841 ccatcaccgc cttctgcacc agttcaaagg aagcccggcc gctcaccatc agggtcgtgc
      901 cgctcagcgg caggcggcct gcgtcgagcg cccaccctat gaccttgtcc acggcgttgt
      961 gcctgccgac gtcctcccgc acgcataaca gttccccgtc ggcgtcgaac aacgcggccg
     1021 cgtgcagtcc gcccgtgcgg tcgaagaccc gctgagccgc cctgagcttc tccggcaacg
     1081 tggtgatcgt tgcggcgttg agccgcagcg gatcacgagc gacgtgccac ttgctgatgg
     1141 tgcgcaccgc ctcgaggctg gccttgccgc acaggccgca cgatgacgtt gtatagaagt
     1201 tgcgttcgag cgacgggtcg ggggcagcca cgccgacttc gaggagcacg tcaagcacgt
     1261 tgtacgtatt gacgccattg gcggtcgctc ccgcgcagta gcggatcccg gcgatgtccg
     1321 aggcggcaga caccacgccc tcgctcacga gaaagcctcg ggccaaatcg aagtcgtcgc
     1381 ctggggtgcg catcgtcacg gtgagcggac ggccggccac cctgatctcc agaggttctt
     1441 ccgcggcgag ggtgtccgga cggtaatcgg ccaccccgtc ggccatgcgc acgactcggt
     1501 atctactggt tacgcgtccc actccggctc ccgaggttgc ttctccgatg gacaagtgat
     1561 ccaggtcacg ctaagctcat cgcatactgt atgcaatagg agtctacatg tcccccacca
     1621 gcgccccagt cgccgtcgtc gaagtcctgg accttcacgg acgctcgtac cggatcggtg
     1681 agcgaccgca cgacatcatc ggtcactccc ggaagtggat gctttggctc tcatgggcgg
     1741 ccatggcggg gatcggcgtg ctgcagtatg gctacggcgt cgccatcacc gcgctgcacg
     1801 tgactgacgg ttcgacagtg acgggcgccc tctgggtgct cgcactgtgg gtggtgttcc
     1861 aggccggcgc ggccgcgcca actgccgcgc tgagccaccg gtttgcactg gccccgtcgc
     1921 gcgccgtgct cgtcggagcc ctgttgtgcg ccgccggccc attgaccctc gcctacaccg
     1981 acagtttcgc gctcgcggtg ctcggttact cggtgctgtc cggtacgggc gccggaatcg
     2041 tctatgccac atgcacgtcc accgttgcga agtggtatcc agagcagcgc gccgtccggg
     2101 tcgggttcgt caccggcgcc ttcggctatg gtgccgtgcc gttcatcgcg ctcttcgccg
     2161 ccgtcctcac accaggaaac cgagcggcaa tcttcgcagg cgttggcgtc tgcgtcctcc
     2221 tcgtcgtcgc cgtgtgcggc gcgctcttgc gcgatccg
//
LOCUS       DASYLX010000571         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2880982,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000571 DASYLX010000000
VERSION     DASYLX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880982"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VGP27_13755"
     CDS             <1..635
                     /locus_tag="VGP27_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEV7852398.1"
                     /translation="IPLAVFPGGTFNHFAKDIGCDSVAKTVEAIRRGDVSCVDLVCLN
                     DEHIVVNTASIGAYPRFVQTREKLEHKIGKPLAGLYAMFHTLRRDEPIKIAYDNKTLQ
                     TSLFFLGNSTYLPSGFAPSRRTRMDDGLMDIRILETGRRFSRLRIVASLALGRLTRSP
                     LYHEMRVPEFAFTAVDGCTELAHDGEIGTECDEARFRVNYRALPVFRPLP"
     gene            complement(589..1260)
                     /locus_tag="VGP27_13760"
     CDS             complement(589..1260)
                     /locus_tag="VGP27_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7852399.1"
                     /translation="MTQRKVWLVGLAAFSLALYVLLWVGIVQQWSWVEIMDRFALDPL
                     HTYGLAHPGWVRFWDVLCTVLGPTVFRLAALVVIIVALARRKVRVAMFLIISVELSGL
                     ITEVAKAAANRQRPAGALVSAQSTSFPSGHALGVMVGVLAFLTLALPIVRRPARFWLI
                     VLGVVVVLAIGAARVVLCVHHPSDVLAGWALGFAWFVGCVLMVPPAVPVTATDEIPEA
                     PGSSL"
     gene            1300..1782
                     /locus_tag="VGP27_13765"
     CDS             1300..1782
                     /locus_tag="VGP27_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852400.1"
                     /translation="MAVFLRRLMRVGGLPAELRTKVDAEGIIYLVEYLPVTRRFSGSV
                     PGKKAKASISSYVGSLVLTPQRVLGTLSSVPKLAGRTIDQRWDAPQSGPVKADISETG
                     VHIEVDIGEVDPKFNGHLSLNYKTAIPDDVLMRLPRRSLAFAVPPEYVLRAVGVPASG
                     "
     gene            complement(2279..2659)
                     /locus_tag="VGP27_13770"
     CDS             complement(2279..2659)
                     /locus_tag="VGP27_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEV7852401.1"
                     /translation="MTQPAKSLYQRMGGYNVIAAVIDDLFAILHSDPAFARFFGGRSN
                     DSVVRSRQLLVDQMCALSGGPCYYIGRDMKTSHSGLGISDVEWETNMKASDAALAKNG
                     VGDAERADFLALFERYRDDIVEAD"
     gene            complement(2764..3306)
                     /locus_tag="VGP27_13775"
     CDS             complement(2764..3306)
                     /locus_tag="VGP27_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852402.1"
                     /translation="MGALGALGARADRLVCGCGSDDCAAAGKPASAVVINVIAEERSL
                     ADDTPVVLDGENPDKPTKPVREMTLAEASVMPKPTGPAHTAPAVMVGGPIIPAPLLAS
                     KIAAGATIRWIAYPGDAPPEPRYRPSEKLALFVRCRDMTCRFPGCHQPADVCDLDHTI
                     AYPAGPTSASNLKCLCRKHV"
     gene            complement(3204..3488)
                     /locus_tag="VGP27_13780"
     CDS             complement(3204..3488)
                     /locus_tag="VGP27_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852403.1"
                     /translation="MEYRARGRHVDRTWSDGNGSSTIEAVLFDHDAAALDSRLDLRPA
                     GSATVTRGRWISDGRMRWVRWARWAPARTGWCAGAGPTTVPRRVSLPARW"
BASE COUNT          691 a         1210 c         1129 g          596 t
ORIGIN      
        1 gaattccact tgcggtcttc cccggcggca ccttcaacca cttcgccaag gacatcggct
       61 gcgacagcgt ggccaagacg gtcgaggcga tccgacgcgg cgacgtgtcc tgcgttgacc
      121 tcgtgtgcct caacgacgaa cacatcgtcg tcaacacggc cagcatcggc gcatacccga
      181 ggttcgtgca gacacgcgag aagcttgagc acaagatcgg caagccactc gcaggcctgt
      241 acgcgatgtt ccataccttg cgccgcgacg agccgataaa gatcgcctac gacaacaaga
      301 cattgcagac gtcgctgttc ttcctcggca attcgacgta tctgccgtct ggcttcgcgc
      361 catcacgacg cacaaggatg gacgacggcc tgatggacat tcggattctc gaaaccggcc
      421 gcaggttcag caggctgcgc atcgtcgcct cgctggccct tggtcgactc acccgcagcc
      481 cgctctacca cgagatgcgg gttcctgaat tcgccttcac cgccgtggac gggtgcactg
      541 agttggcgca tgacggcgag atcggcaccg agtgcgacga ggccaggttc agagtgaact
      601 accgggcgct tccggtattt cgtccgttgc cgtgaccgga acggcgggcg gcaccatcag
      661 cacacagcct acgaaccacg caaaacccag cgcccacccg gccaacacgt cggatgggtg
      721 atgcacgcac agcaccacgc gtgccgcgcc gatcgcgagc acgacaacta cgccaaggac
      781 gatcagccag aaccgggccg gcctacgcac gatcgggagg gcgagcgtca gaaatgccag
      841 cacgccgacc atcaccccga gagcgtgtcc cgacgggaat gacgtcgact gcgcgctcac
      901 cagggcaccg gccggtcgct ggcggttcgc ggccgccttg gcgacttcgg tgatcaaccc
      961 gctgagttcg acgctgatta tcaggaacat cgccactcgc accttgcgtc gggccagggc
     1021 aacgatgatc acgaccaacg ccgccagccg gaaaactgtg ggcccgagca ctgtgcacaa
     1081 cacgtcccag aagcgaaccc aacccgggtg ggcgagcccg taagtgtgca gaggatcgag
     1141 ggcgaaccgg tccatgattt cgacccacga ccactgctgc acaatgccga cccacagcag
     1201 gacgtacagc gcaagcgaga acgcggcaag gccgacgagc cacactttcc gctgggtcac
     1261 cacccccaac gactaccacg ctttcgctac ggtgaaccta tggctgtgtt tctgcgcaga
     1321 cttatgcgcg tcggcggact acccgctgag ctgcgcacca aagtcgacgc tgagggcatc
     1381 atctacctgg tcgaatacct cccagtgact cgacgattca gcggatccgt gcccggcaag
     1441 aaggcgaaag caagtatctc gagctatgtc ggctctcttg tgctcactcc gcagcgcgtg
     1501 ctcggcacgt tgtcgtcggt gcccaagctg gcgggccgga ccatcgacca acgctgggac
     1561 gcaccccaga gcggacccgt caaggccgac atctcggaga ccggtgttca catcgaggtc
     1621 gacatcggcg aggtcgaccc gaagttcaac ggccacctct cgctgaacta caagaccgcc
     1681 atccccgacg acgtcctgat gcgactgccg cgtcggtcgc tcgccttcgc ggttccgccc
     1741 gagtacgtgc tgcgtgcggt cggcgttccc gccagcggat agcggccgtg tcgaaggacc
     1801 actcagaacg gcggcgggtc gtcatcgccc ggtggtaacg acgcgaacca tgacgcgaag
     1861 ctccccgccg gggcgcgcgc cgaccagacg gcggtttcgc gcggttgcgg ctgggtctcg
     1921 ttgaggcgcc gttcagccgc gatccgcgcg gcgcggtctt gtgcgcgagt gcggcggcgg
     1981 tgcggcatgg ctagtccgcg acccggctga gcctcggctg cggcgaccgc cgctgccact
     2041 tcggcggcgg acaacacgac gtgagcggtc ggccgacaca gggacggaaa cataatccgg
     2101 ctgtctggtt gagtggtgtg cgtctgactg cccggcgacg tccacaccac agtgccatcg
     2161 ggcagttgtc gatctcgcca cccccagaaa gtcttgagga ggtggtggca gcgacttctt
     2221 gaaagacact ctagaagagc gaccggggcg ggcccgcaac atccagatag gttgttgatt
     2281 aatctgcctc gacgatgtcg tcgcggtaac gctcgaacag cgcgaggaag tcggctcgct
     2341 cggcgtcgcc tacgccgttc ttggctagcg ctgcatccga agctttcata ttcgtttccc
     2401 actcgacgtc actgatcccg agtccgctgt gcgatgtctt catgtcgcgg ccgatgtaat
     2461 agcacggtcc accacttagc gcacacatct ggtcgaccaa gagctgccga ctgcgaacga
     2521 ccgagtcatt gctgcgacca ccgaagaagc gagcgaaagc ggggtcgctg tgcaaaatgg
     2581 cgaacagatc gtcgatgacg gcagcgatca cgttgtagcc gcccatccgc tgatagaggg
     2641 acttcgccgg ctgggtcatg attggcctcc aacaagggtg aagcgttagg cctcagggta
     2701 gggcacgacc gacacaaaac cccgaacatc gtcatcacat cggaattggc gaagagcatt
     2761 cgactagaca tgctttcggc ataggcattt gagattggac gcacttgtcg gcccggcggg
     2821 gtaggcgatc gtgtggtcca gatcgcagac atctgcgggc tggtggcagc cggggaaccg
     2881 gcatgtcata tcgcgacacc gaacaaacaa cgcgagtttc tccgacggcc gataccgcgg
     2941 ttcgggtggt gcgtcgccgg ggtaggcgat ccaccggatg gtcgcgcctg cggcgatctt
     3001 ggaggccaac agcggcgccg gaatgatcgg gccgccgacc atcacggccg gagcggtatg
     3061 cgccgggccc gtcggcttcg gcatcaccga cgcttcggcg agggtcatct ccctgactgg
     3121 cttcgtcggc ttgtcgggat tctcaccgtc cagcaccaca ggggtgtcgt cggcaaggct
     3181 tcgctcctcg gcgatcacgt tgatcaccac cgcgctggca ggcttacccg ccgcggcaca
     3241 gtcgtcggac ccgcacccgc acaccagccg gtccgcgcgg gcgcccagcg cgcccaacgc
     3301 acccaacgca tccgcccgtc gctgatccag cgtccgcggg tcactgtcgc agaccccgcg
     3361 ggcctcaggt cgaggcgtga atccagggcg gcagcgtcgt ggtcgaacaa caccgcctcg
     3421 atcgtcgagg agccattccc gtcggaccac gtccggtcca cgtggcgacc gcgcgcccgg
     3481 tattccatcc gacgtaacgc gtaagggtcg tagcggtcga cccagttgtc aatggcctgc
     3541 tcgacttacg cctcggacag tgcgccccac cccgtcaccg ctgcggccaa gtcgatgtcg
     3601 actttggcgc gggcatgcgg atcggc
//
LOCUS       DASYLX010000572         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2660074,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000572 DASYLX010000000
VERSION     DASYLX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660074"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGP27_13785"
     CDS             complement(<1..324)
                     /locus_tag="VGP27_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEV7852404.1"
                     /translation="MTVGLDPEATRPELTDGEGGAPGLFDRRYGTTSLRVGAASIWLS
                     VIVLLPLAAILWQAAGGGWSAFWSTVTSKAAVESFRVTLTVSIGVSLINLVFGLLVAW
                     VLTRDD"
     gene            complement(321..1346)
                     /locus_tag="VGP27_13790"
     CDS             complement(321..1346)
                     /locus_tag="VGP27_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7852405.1"
                     /translation="MKIRKAWLNAVVLATTATVLAACGGGSSDVAGGGDQPAADTTLT
                     LVAYAVPEPGWSKIIPAFAATPEGKGVAVTTSYGASGDQSRAVQDGKPADIVNFSVEP
                     DVTRLVKADKVAKDWNADATKGIPFGSVVSLVVRKGNPKNIKDWDDLLQPGLEVVTPS
                     PLSSGSAKWNLLAPYAAKSDGGKNGQAGLDYVTKLVTEHVKTRPGSGREATDVFLQGT
                     GDVLISYENEAINVERQGKPVEHINPPQTFKIENPVAVVTSSAHADKANALKNFLYTP
                     EGQKVWAQAGFRPVDPAVAEDFANDFPTPQKLWTIADLGGWSSVDPALFDKENGSITK
                     IYKQATG"
     gene            complement(1699..1893)
                     /locus_tag="VGP27_13795"
     CDS             complement(1699..1893)
                     /locus_tag="VGP27_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852406.1"
                     /translation="MGGFLNWWDGVELWLSGLGFVAQTAVVMPVVLALAYGIAILLDG
                     ALGNGIRLLRRARHQDEDGE"
     gene            2009..>2556
                     /locus_tag="VGP27_13800"
     CDS             2009..>2556
                     /locus_tag="VGP27_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEV7852407.1"
                     /translation="MVLQHSEAASEAPGPPPLTVTAPVPYPPNGALRNPFPPIADYAF
                     LSDCENTCLISSAGSVEWLCVPRPDSPSVFGAILDRGAGHFRLGPYGVSVPAARRYLP
                     GSLILETTWQTHTGWVIVRDALVMGPWHDLESRSRTHRRTPMDWDAEHILLRTVRCVS
                     GTVELVMQCEPSFDYHRVSASW"
BASE COUNT          443 a          896 c          799 g          418 t
ORIGIN      
        1 gtcgtcgcgg gtgagtaccc acgccaccag caatccgaag acgagattga ttaggctcac
       61 gccgatcgag accgtgagtg tcacccggaa cgactcgacg gccgccttcg atgtgaccgt
      121 cgaccagaag gcactccagc cgccaccggc cgcctgccac aggattgccg cgagcggcag
      181 cagcacgatg acgctcagcc agatcgacgc cgcccccacc cgaagcgacg tcgtgccgta
      241 ccgcctgtcg aacagcccgg gcgccccgcc ctcaccatcg gtcagttcgg gccgggtcgc
      301 ctcggggtcg agcccaaccg tcatccagtg gcctgcttgt agatcttcgt gatggagccg
      361 ttctccttat cgaagagcgc gggatccacc gagctccagc cgccgagatc agcgatggtc
      421 cacagcttct gcggcgtcgg aaagtcgttc gcgaagtcct cggcgaccgc cgggtcgacc
      481 ggccggaacc ctgcctgtgc ccagaccttc tgcccctcag gggtgtagag gaagttcttc
      541 aacgcattgg ccttgtccgc gtgggcgctc gacgtgacga cggcgaccgg gttctcgatc
      601 ttgaaggtct gcggcgggtt gatgtgctcg acaggcttgc cctgccgctc gacattgatg
      661 gcctcgttct cgtagctgat cagcacatcg ccggttccct gaaggaatac gtcggtggct
      721 tcgcggcccg aaccgggtcg cgtcttgacg tgctcggtga ccaacttggt gacgtagtcg
      781 agtccggcct gcccgttctt gccgccatca ctcttggcgg catacggcgc caacaggttc
      841 cacttcgccg agccggaact cagcgggctc ggcgtgacca cctcgagccc gggctgcagc
      901 aggtcatccc agtctttgat gttcttcgga ttgcccttgc ggacaacgag cgaaaccacc
      961 gaaccgaagg ggatgccctt ggtggcgtcg gcgttccagt cctttgccac cttgtcggcc
     1021 ttcaccaggc gagtgacgtc cggctcgacc gagaagttga cgatgtcggc cggcttgcca
     1081 tcctgcacgg ctcgcgactg gtcaccggag gcaccgtacg aggtcgtcac ggcgacaccc
     1141 ttgccctccg gggtggccgc gaacgccgga atgatcttgc tccaacccgg ctcgggcacc
     1201 gcgtaggcga cgagcgtcaa ggtggtgtcg gcggccggct gatcgccgcc accggcgacg
     1261 tcactggagc cgccgccgca cgccgcgagc actgtcgcgg tggtagcgag cacgacggca
     1321 ttgagccagg ctttgcggat cttcattgat ggccttcctt gcgtggacga gacggacggg
     1381 taagtccgcc acgacggcaa ggcgaaggat cagtatccgg gcacaggaac agccgctacc
     1441 gacaccaaac cgcggacgga ggtgacttca gcgacaacaa cagacgtcag caacagcagt
     1501 gaaccccacg gcaatgagga ccaggacatg gccgctcttg ttgccggacg ctgtgcgcat
     1561 ggcgcgaaga ataacagagt ccggctaatt gctcagatcg gggctttcgc cgacgtcaac
     1621 gtgtgacgaa ccacatgacg accaccagcg ccacgaggat gaccagtacc aacgtgaccc
     1681 gagaccgggg catcgctctc actccccgtc ctcatcctgg tgccgcgccc tccggagaag
     1741 ccggatcccg ttgcccagcg caccgtccag caggatcgcg atcccgtagg ccagcgcaag
     1801 caccaccggc atcacgaccg cggtctgggc gacgaagccg agcccggaaa gccacagctc
     1861 aacgccgtcc caccagttga ggaagccgcc cacccagcca gcctacggtg cgagcgcctg
     1921 cccgcctgcg cccgacgccg cgccagcaac gccggacaca cccctcgtca agtagacgcc
     1981 gggcaggttc ggagtcgatg atgtccgaat ggttctgcaa cacagtgaag cggccagcga
     2041 ggccccgggg cccccgccct tgaccgtgac ggccccggtg ccgtacccgc cgaatggagc
     2101 gttgcgaaac ccgtttccgc cgatcgccga ctacgcgttt ctctccgact gtgagaacac
     2161 ctgcctgatc tcgtcggccg gctctgtcga atggttgtgc gtcccgcggc cggactcgcc
     2221 cagtgtgttc ggcgcgatcc tggaccgcgg cgccggccac ttcaggctcg gaccctacgg
     2281 cgtctccgta cccgccgcgc ggcgctatct tcccggcagc ctgatcctgg agacgacctg
     2341 gcagacccac accggctggg tcatcgtccg tgacgcgctg gtgatgggcc cctggcacga
     2401 cctcgagtcg cggtcccgta cgcatcgccg cacgccgatg gactgggacg ccgagcacat
     2461 cctgctgcgc accgtgcgct gtgtgagcgg caccgtcgag ctggtgatgc agtgcgagcc
     2521 ctccttcgac taccaccgcg tcagcgccag ctggga
//
LOCUS       DASYLX010000573         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1233984,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000573 DASYLX010000000
VERSION     DASYLX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233984"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGP27_13805"
     CDS             complement(<1..288)
                     /locus_tag="VGP27_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7852408.1"
                     /translation="MGTSVAVVGPGAIGSTMAALLHAAGHHVLLCGHTPRDRVEVRPD
                     DRDPIVIPGPVHTDPADVDGPVDVVLLAVKDTQNRNASGWLARLCDDGTVVC"
     gene            366..>1898
                     /gene="aspS"
                     /locus_tag="VGP27_13810"
     CDS             366..>1898
                     /gene="aspS"
                     /locus_tag="VGP27_13810"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV7852409.1"
                     /translation="MLRSHAAGSLRAADAGQKVTLAGWVARRRDHGGVIFIDLRDASG
                     VSQVVFREADVLQAAHRLRAEFCVAVEGTVETRPEGNANPEIATGDIEVNATSLTVLG
                     ESAPLPFQLDETAGEEARLKYRYLDLRREGPGNAIRLRSKVNAAARAVLAAHDFVEIE
                     TPTMTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDE
                     DFRADRQPEFTQLDMEMSFVSTEDVIAISEEIITELWALIGYDVPTPLPRMTYADAMR
                     RFGSDKPDLRFGLELVECTDFFADTPFRVFQAPYVGAVVMPGGASQPRRTLDAWQDFA
                     KQRGHRGLAYVLVGDDGRLGGPVAKNLSDAERDGLAARVGASPGDCIFFSAGPPKPSR
                     ALLGAVRIEVAKRLDLIDPNAWAFTWVVDPPLFEPADEATAAGDVAVGSGAWTAVHHA
                     FTAPKPEYEDAIDSDPGSILADAYDIVCNGNEIGGGSIRIHRRDVQEQVFAVMGLDKA
                     QAE"
BASE COUNT          304 a          648 c          638 g          308 t
ORIGIN      
        1 gcacaccacc gtgccgtcgt cgcagagccg cgccaaccat cccgacgcgt ttcggttctg
       61 ggtgtccttg accgccagca acaccacatc gacgggtccg tcgacgtcgg cggggtcggt
      121 gtgcactgga ccgggtatga cgatcgggtc cctgtcgtcg ggcctgacct ccacccgatc
      181 gcgcggcgtg tggccgcaca gcagcacatg atgacctgcc gcatgcagta gagccgccat
      241 cgtcgagccg atcgccccgg ggccgacgac cgcaacgctg gtacccacaa ggccaagcta
      301 ccggcggatt cgctgccact acactgtgga gtcgtctcgt caggccattc ccagggagat
      361 ttctcgtgct gcgcagccat gccgccggtt cgctacgggc cgccgatgcc ggtcagaagg
      421 tgacgttggc aggctgggtt gcccggcgcc gcgaccacgg cggcgtgatc ttcatcgacc
      481 tgcgcgacgc ctccggcgtt tcgcaggtgg tcttccgcga ggccgacgta ctgcaggcgg
      541 cgcatcggct gcgcgccgag ttctgcgtcg ccgtcgaggg caccgtcgag acccggcccg
      601 agggcaatgc gaacccggag atcgccaccg gtgacatcga ggtcaacgcg acgtcgctaa
      661 ccgtgctggg ggagagcgcg ccgctgccct tccagctcga cgagacggcg ggcgaggagg
      721 cccggctgaa gtaccgctac ctcgacctgc gccgcgaggg tcccggtaat gcaatccggt
      781 tgcggtccaa ggtcaacgcg gccgcgcgcg ctgtactggc tgcgcacgat ttcgtcgaga
      841 tcgaaacgcc gacaatgacc cggtccaccc cggagggcgc ccgcgacttc ctggtccccg
      901 cccgcctgca gccggggtcg ttctacgcgc tgccgcagag cccgcagctg ttcaagcagc
      961 tgctgatggt ggcaggcatg gagcgctact accagatcgc gcggtgctac cgcgacgagg
     1021 acttccgcgc cgaccgtcag ccggaattca cccagctcga catggagatg agcttcgtca
     1081 gcaccgagga cgtgatcgcg atctccgaag agatcatcac cgagttgtgg gcgctgatcg
     1141 ggtatgacgt gcccacccct ctgccacgga tgacctacgc cgatgcgatg cggcgcttcg
     1201 gctccgacaa gcctgacctc cgattcggcc tggaactcgt cgagtgcacg gacttcttcg
     1261 ccgacacccc gtttcgggtg tttcaggcgc cctacgtcgg cgcggtcgtc atgccgggag
     1321 gtgcgtcgca gccacgccgc acgctggacg cctggcagga cttcgccaaa cagcgtggac
     1381 accgcggact ggcctacgta ttggtgggcg acgacgggag gctcggcgga cccgtcgcca
     1441 agaatctttc ggatgccgaa cgggacggtc tcgcagcacg tgtcggcgcg tcacccggcg
     1501 actgcatctt cttctcagcg ggtccgccga agccttcgcg ggccctactc ggcgccgtgc
     1561 gcatcgaggt cgccaagcgg ctggacttga tcgatcccaa cgcgtgggcg ttcacgtggg
     1621 tggtcgaccc gccgctgttc gagccggccg acgaggcgac cgccgcgggc gacgtcgctg
     1681 tgggatcggg tgcatggacc gcggtccacc atgcgttcac cgcacccaag cccgagtacg
     1741 aggacgcgat tgacagcgat ccgggcagca tcctggccga cgcctacgac atcgtgtgca
     1801 acggcaacga gatcggtggt gggtcgatcc gtatccatcg tcgcgacgtc caggagcagg
     1861 tgttcgcggt gatgggcctc gataaggcgc aggccgag
//
LOCUS       DASYLX010000574         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_687135,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000574 DASYLX010000000
VERSION     DASYLX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687135"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGP27_13815"
     CDS             complement(<1..1139)
                     /locus_tag="VGP27_13815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7852410.1"
                     /translation="MPLAAGQTFAGYTIVRLLGTGGMGEVYLAEHPRLPRSDALKVLP
                     ADVCADHEYRERFNREADIAATLWNPHIVGVHDRGDVDGQIWISMDYVDGTDAAELLR
                     DRYPKGMPKEAVLDIVTSVADALDYAHQQGLLHRDVKPANILLTDAAAGEQRILLGDF
                     GIARRVDDTSNLTATNMTVGTVYYAAPEQLMGDELDGRADQYALAATAFHLLTGSPPF
                     QHSNPTVVISQHLTASPPAIADKRPELSALDPVLCKALSKDAKDRFTSCMDFARALAH
                     RLGVQPEADNERTAASTPIAPRSHGGRHEAQPAPRRKYLRPAYIVPGVLAALLLVAIA
                     LVLIQFREIEKLLNDDYTPSPRPPATSPRAEAPPALPPPPSESTPS"
     gene            complement(1160..2266)
                     /locus_tag="VGP27_13820"
     CDS             complement(1160..2266)
                     /locus_tag="VGP27_13820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEV7852411.1"
                     /translation="MGLLDRYRQAPPSVWGRRRHDVVLGIADASVSALWTFSGAIRKM
                     KPPPAPDRVLGLTVLARQVVAHDQDVVALTLAAADGQPLPRWRPGAHLDIHLPSGRLR
                     QYSLCGDPDDDETYRIAVRRIVEGGGGSIEVHDGLPIGTTVTTHGPRNAFPLTVPGYG
                     SPATRFRFVAGGIGITPILPMLALADRLGVDWSMVYAGRSHDSLPFIDEVSRFGDRID
                     IRTDDVSGIPTAAELLGDCPDATTVYACGPAPMLTAIRAELAGRDNVELHFERFAAPP
                     VVDGRQFTVSIASTGQEVTVGADETLLSALRRANVAAPYSCRQGFCGTCRTRVVAGAV
                     DHRDTLLTEPERAASMMLVCISRAANGERLTLDL"
     gene            complement(2266..>2364)
                     /locus_tag="VGP27_13825"
     CDS             complement(2266..>2364)
                     /locus_tag="VGP27_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV7852412.1"
                     /translation="YFRPGFTPEEMGSTAQAVAYLASSPAARAAHI"
BASE COUNT          379 a          743 c          856 g          386 t
ORIGIN      
        1 gacggcgtcg actcggacgg cggcggcggt agtgccggtg gcgcttcggc ccgaggggag
       61 gtcgcgggcg gcctcggcga cggggtgtag tcgtcgttca gcagtttctc gatctcacgg
      121 aactggatca gcacgagggc gatggcgacc agcaacagcg ccgcaaggac gccgggaacg
      181 atgtaggccg ggcgcaggta tttccgccgc ggcgcaggct gggcttcgtg cctgccccca
      241 tgcgatctgg gggcgatggg agtcgacgcc gccgtgcgct cgttgtcggc ctccggctgg
      301 acccccagtc tgtgggcaag agcccgcgcg aagtccatgc aactggtgaa ccggtccttg
      361 gcgtccttcg acagcgcctt gcacaggacc ggatcgagcg ccgacaactc gggacgcttg
      421 tcggcaatgg cgggcggtga cgccgtgagg tgctggctga tgaccaccgt cggattcgaa
      481 tgctgaaacg gcggcgagcc ggtcagcagg tgaaatgccg tggccgcgag cgcatactga
      541 tccgcgcggc cgtcgagttc gtcacccatc aactgctcgg gagcggcgta gtacacggtg
      601 ccgaccgtca tgttcgttgc ggtgaggttg ctggtgtcgt cgacccgtcg agcaataccg
      661 aagtcgccga gaagtattcg ctgctcgcct gcggccgcgt cggtcaacaa gatgttggca
      721 ggtttgacgt cgcgatgcag aaggccctgc tggtgtgcat agtcgagggc gtcggcgacg
      781 gaggtgacga tgtcgagtac tgcctccttg ggcatgccct tcgggtagcg gtcgcgcagc
      841 agttcggccg cgtccgtccc gtcgacgtag tccatcgaga tccagatctg gccgtcgacg
      901 tccccgcggt cgtgcacccc gacgatatgg ggattccaca gtgtcgcggc gatgtcggct
      961 tcgcggttga accgctcccg gtattcgtgg tcggcgcaga catcggcggg caataccttg
     1021 agggcgtcgc tccgcggcag gcgcggatgt tcggcaaggt agacctcgcc catgcctccg
     1081 gtacccagaa gccgcacgat ggtgtagccg gcgaaggtct gaccagcggc caatggcatg
     1141 gccgaatagt acagacgggt tacaaatcca gcgtgagtcg ctccccgttc gcggcccgcg
     1201 agatgcagac gagcatcatg gaagccgccc gctccggttc ggtcagcaac gtgtcgcggt
     1261 ggtccacggc gcccgcgacg acgcgggtcc ggcaggtgcc gcagaagcct tgccggcatg
     1321 agtaaggcgc cgcgacgttc gcccgccgca acgcgctcag cagcgtctcg tcggcgccga
     1381 ccgtgacttc ctggccggta gaggcgatcg agacggtgaa ttgcctgccg tcgacgacgg
     1441 gcggagcggc gaaccgttcg aagtgcaact cgacgttgtc gcgtcccgcc agctcggcgc
     1501 ggatagcggt caacatcggc gcggggccgc atgcgtagac cgtcgtcgcg tcgggacagt
     1561 cgccgagcag ctcggccgcg gttggtatgc cgctgacgtc gtcggtacgg atgtcgatgc
     1621 gatcgccgaa gcggctgact tcgtcgatga acggcaggct gtcgtggctg cggccggcgt
     1681 acaccatcga ccaatccacg ccgagacgat cggcgagcgc cagcatcggc aggatgggcg
     1741 tgatgccgat tccgcccgcg acgaaccgga accgggtagc gggggagccg tagcccggga
     1801 cggtgagtgg gaaggcgttg cgcggcccgt gggtggtgac tgtggtgccg atcggcaacc
     1861 cgtcatgcac ctcgatcgat ccgcctccgc cctcgacgat gcggcgcacg gctattcggt
     1921 aggtttcgtc gtcgtcgggg tcgccgcaca acgagtactg tcgcagccgg ccgctgggca
     1981 gatggatgtc gaggtgtgca ccggggcgcc agcgcggcag cggttggccg tcggcggccg
     2041 ccaacgtcag cgccaccaca tcctggtcgt gtgcgaccac ctgacgggcg agaacagtca
     2101 ggccgagaac gcggtccggt gccggtggcg gtttcatctt cctgatggca ccggagaacg
     2161 tccagagcgc actgaccgac gcgtcggcga taccgagcac gacgtcgtgg cgcctgcgcc
     2221 cccaaacact cggcggcgct tggcggtagc ggtcgagcag gcccatcaaa tgtgtgccgc
     2281 gcgagcagcg ggagagcttg cgaggtaagc caccgcctgc gccgtcgaac ccatctcctc
     2341 cggggtgaac cctggccgga agta
//
LOCUS       DASYLX010000575         9867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_687140,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000575 DASYLX010000000
VERSION     DASYLX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687140"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGP27_13830"
     CDS             complement(<1..191)
                     /locus_tag="VGP27_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852413.1"
                     /translation="MAKTTENQQTTDTQTTTGNQDSTTGNQSTDLPAAPGGNPDNRET
                     VQNMGEPELRNAVIELLTI"
     gene            complement(300..496)
                     /locus_tag="VGP27_13835"
                     /pseudo
     CDS             complement(300..496)
                     /locus_tag="VGP27_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072061.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
     gene            complement(553..1617)
                     /locus_tag="VGP27_13840"
     CDS             complement(553..1617)
                     /locus_tag="VGP27_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7852414.1"
                     /translation="MCGDGGGLADEVAASTPRRAEGPAVDPIALEPVDEVIVTTLMDN
                     SYDALMGDTGPARRTSFTHLSGVAAPQYVDGCTVPGLVAEHGFSALVTTRRGNATHTV
                     LFDTGVSPDGMATNIERLGLDVAAIEVLVLSHGHFDHAGGFPGLARLRGRSGMPLTVH
                     PLVWSRRRLALPDQHHWNMPTLSRAALEAEGLEVIERRQPSLLLDKSVLITGEVDRTT
                     DFEHGMPNHEAWHDGRWEPDPLILDEQGLVVHVRGRGLVVITGCGHAGAVNIARHAMR
                     LTGVDRLHALLGGFHLTGPAFEPIIEPTVAAFTDMAPDVLVPAHCTGWKAQHRLAAAL
                     PAAFVPNAVGTSFALAGQAA"
     gene            2534..3496
                     /locus_tag="VGP27_13845"
     CDS             2534..3496
                     /locus_tag="VGP27_13845"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852415.1"
                     /translation="MSVVLDTGQIPKADRAEAVRAAVAEHLVPVEIDFAADRGPAAVP
                     MAITDLADLTVWTSTSHAVKVHRTAAQTHDDSTPSIFMGLQMTGSCVVVQHGRQVILR
                     RGDLVIYDSTSPFLISDVDGIRQHKFRIPLDRLALPADVIRRVCAVSLSPGHPITDLA
                     ATYLHRLALRPGMFDRPGGDLVSQPSIELLRAVITTHLDAVELGKESLHATLVVRIME
                     YVRTHLREPQLSAGRIAAEHHISVRQLYRILADEGISLGDWIRARRLEECRKDIASTG
                     AGDPVFVVARRWGFTDASSFARMFRATFGMSPREWRETNRRPPH"
     gene            3660..5357
                     /locus_tag="VGP27_13850"
     CDS             3660..5357
                     /locus_tag="VGP27_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852416.1"
                     /translation="MPDRDRQPSEPTGASPWAGSAPAIPQLSAAQFDRMAAYGVARPV
                     ELGDIVFGPGDVDYDLILVESGWIEIVSPVAGDEPESVVARYGPGGVLGELNFLTGQT
                     AYLMARVIEAGRIYRITRAQFRRLMATEPDLSDILLRTLLARRDLLRDGPAARAIAIL
                     GSGLSGDSLALRTFAARQRLAYLWLDADSAAGRALAESARITGADLPAVITGHQVMRR
                     ATTGQMAQALGLAYRHTGTKPSDLIVVGSGPAGLGAAVYGASDGLDTVVLDAAGVGGQ
                     AAASSRIENYLGFPSGLSGQELTQRAALQAMKFGAQLSSPCRVVDLDTGGAHLTVVLS
                     DGTTIESRAVLIATGVHYNTLPLERWIEFEGAGIYYAATELEARACRTEPVTVVGGAN
                     SAGQAALFLALRGCQVTVAVRGSDVAATMSSYLLGRLRADPRVTIRVDTEVTGLSGGS
                     SLQRITLTNNSSGEVSEHLCAGLFCFIGARPETEWLHDVAVDSAGFIRTDAHLDPEEL
                     GTTWSALGRPPLPFETSMPTVFAAGDVRLSSVKRVASAVEEGASAVRSIHTALGPHT"
     gene            5797..6729
                     /locus_tag="VGP27_13855"
     CDS             5797..6729
                     /locus_tag="VGP27_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7852417.1"
                     /translation="MPLTTESGLDGEYVPSPMESVRMQVAGYEASGGVKGGTLEGRPV
                     VILTSVGAKSGKVRKNPVMRIVDGDRYVAVASAGGSPTNPSWYANLVAHSRVHLQDGA
                     NVREFAAREVAGDEKRYYWAVAERFRPHFPEYRRLAGDRDIPIMVLEPIAPRTPVTPF
                     VRYGSIPNPYQGGTAMTTGAEERNIAVVLEAFDTLFNRRDYAAAQRFWSPDYIQHSAH
                     IEPGREGLFELVKASPPDMRYENGLIVANGDYVMLHGRFTDIGQPANWIAADIVRLEN
                     GLLAEHWDVLQDEATAEESVSGLPMFGETFPTRV"
     gene            6861..7610
                     /locus_tag="VGP27_13860"
     CDS             6861..7610
                     /locus_tag="VGP27_13860"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7852418.1"
                     /translation="MSRLDGKIAVITGGSSGIGLATAQRFVQEGAYVFITGRRQSELD
                     KAKALIGDGVTTVAVDSTSSADQDTLFATVLEEKGALDILVTNSGRVEPEELGKITEE
                     NFDLTFDLNARATLFTVQKAVPLMRDGGSVILIGSVAGYTGINGYTTYSATKAALRSY
                     TRTWTRESKDRGIRFNTLSPGPIDTPIMDLQADSPEGADAIRAAFAAAIPLNRMGRPE
                     EIAAAALFLASDDSSFVAGAELSVDGGFAQV"
     gene            7807..8583
                     /locus_tag="VGP27_13865"
     CDS             7807..8583
                     /locus_tag="VGP27_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV7852419.1"
                     /translation="MTAVSPTPDRDYPQLLGERGLIEPAPRRVRGYFDKTLVFDTARA
                     HYVWEIAYYPQYYIPTPDVRMDHLRDENHVQKLQFGSSRLYSLTSAERTWPSAARVYD
                     SGPVAGSVRFEWDALDWFEEDEPLILHPRNPYVRVDSLRSHRHIRVELDGVVLAETHS
                     PVLLFETGLPTRFYIDRTDVEFSQLEPSKTESVCPYKGITSAYWTFRNGDAVHPDIAW
                     AYDYPLREVAPIAGMIAFYNEKLDIFVDGALLSRPHTIFT"
     gene            complement(8754..9365)
                     /locus_tag="VGP27_13870"
     CDS             complement(8754..9365)
                     /locus_tag="VGP27_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852420.1"
                     /translation="MHVRYTLITADPERIDDAVAYLASDGRSRLESEPGEQGMSLQVS
                     PDLAVAVLESFWVSHDAMRESERVEAGVRDEAVRLALGTASVERFRRASVTRVGREEG
                     GAGVRHTVYQTNPAALDQAIAGYEDTAVPWLTETDGFVSAALLVDHRSGRQLSQTIWR
                     DIGALVKSRSPAAAIRVDAVAATDSAVVALDEYGLVFRATRAE"
     gene            complement(9384..>9867)
                     /locus_tag="VGP27_13875"
     CDS             complement(9384..>9867)
                     /locus_tag="VGP27_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV7852421.1"
                     /translation="SLEHELAAPMSVEHAADNLGNYDAVFIPGGHGPMEDLPDCRPLG
                     RILADLFDTGRVVAAVCHGPAGLLAARREDGTWLFAGRRLTAFTNEEERQAGLADRAK
                     WLLETRLREEGADFDAGQPWSSHVVVDGHLVTGQNPASSKHAAERTLAVLMAAQSPPS
                     "
BASE COUNT         1717 a         3226 c         3163 g         1761 t
ORIGIN      
        1 atgatcgtta acagctctat cacggcattg cgtagttcgg gttcgcccat gttctgaacg
       61 gtttcgcggt tgtccgggtt gcctccgggc gccgccggta ggtctgtgga ctggtttccg
      121 gtggtgctgt cttggtttcc ggtggtggtt tgggtgtcag tggtctgctg gttttctgtg
      181 gtttttgcca tgagatctac ggtattcact aattcgtcgg cagtagacga cccggcaggt
      241 cgattctgag cccccgactt tgtcatgagg aatcacttcg accatgcgcc gaggcggccg
      301 gggcggtgca tcaggatcac catgtccgca tcctgctcga tggaccccga ttcacgaagg
      361 tcctatagcg tgggcttctt gtccgtgcgc tgctccggac cgcggttcag ctggctcatc
      421 acgaccaccg gacctccagc ttcttcgcca aaagcttgag ctgacgggac aattcggaga
      481 cttcctgctc gcgcgagatg aatccgaccc gcgaagaatt tcgtcctcga cttggcgccg
      541 gcacgaggat ccctaggctg cttgtcctgc cagcgcgaac gaggtaccga ccgcattcgg
      601 cacgaacgcg gcgggcaacg ctgcggcaag gcggtgctgg gccttccagc cggtgcagtg
      661 cgcggggacc agcacgtcgg gcgccatgtc ggtgaatgcg gcgacggtgg gttcgatgat
      721 gggttcgaac gccgggccgg tgaggtggaa gccgccgagc agtgcatgca gtcgatccac
      781 gccggtcaac cgcatcgcgt ggcgggcgat gttcaccgcg ccggcgtgtc cgcatccggt
      841 gatgacgacg aggccacgtc cgcggacgtg caccaccagc ccttgctcgt cgaggatcag
      901 cgggtccggt tcccaacgtc cgtcgtgcca cgcctcgtga ttcggcatgc cgtgctcgaa
      961 gtcggtggtg cggtcgacct ctccagtgat cagtaccgac ttgtccagca gcagcgaagg
     1021 ttgccgccgt tcgatcacct cgagtccctc ggcctccagg gccgcgcggc tcagggtcgg
     1081 catgttccag tggtgttggt cgggtagggc gaggcggcgc cggctccaca ccagagggtg
     1141 caccgtgagc ggcattccgc ttcgtccgcg cagccgcgcc aggccaggaa agccgccggc
     1201 gtgatcgaag tgaccgtggc tgagcaccag gacctcgatc gcggccacgt ccaggccgag
     1261 gcgctcaatg ttggtggcca tgccgtccgg ggagaccccg gtatcgaaca agactgtgtg
     1321 cgtggcgttt ccgcggcggg tcgtgacgag cgccgagaac ccgtgctcgg ccaccaagcc
     1381 aggtactgtg cagccgtcta cgtactgcgg ggccgcaaca ccagatagat gggtgaacga
     1441 ggtacggcgg gcgggtccgg tgtcacccat cagcgcgtcg tagctgttgt ccatcaaggt
     1501 ggtgacgatc acctcatcga ccggttccaa ggcgatcggg tccaccgccg ggccctccgc
     1561 tcgccgcggt gtggaggcgg ccacctcgtc ggcgagcccg ccaccgtcac cacacatgcc
     1621 ttcagtgtgc gcgtcttcaa cctcggcggg taggtgtgtt acatcctcag tacgtgcaca
     1681 cgtccgtgca catccgcgat gggcaagtcc ggctctgccg acttaacccc cgcgcagttg
     1741 ccgcagtcct acgcgaccga ggaaccccac atagacgttc acgtgatcga atctcgcctc
     1801 gaacaggttc aggatcatgg gctttttgct agctttttca ccgcggccgc accgcggttt
     1861 gtctcacgaa ccgcttcatt tcctggagtg ggtggcgcct gtcttatcag ccgcgctacc
     1921 cgagtctgct cgacgccctc ggtgcgtcgt tgtcacccac cgatgcgttg atgccggcca
     1981 tgtcctgcgc gagttcggaa gtcgccagtg gtctttgaga aagatggcgg cgccaatcac
     2041 cagattccgc tgatgcccga ttacgtcgct ggcccgacgc aggatctact tgagcgcgcg
     2101 gagcggcgtc acgtgacccg ggcgggtggc gacgtccgcc gcacggcacc gatcagcccg
     2161 gtggtgcggc ccgcgaacac gctgaccgcg cggtggtgca ctcattcata aacaacatcg
     2221 gttagacgcc tggcctcccg tgattggaaa cgagcggaca ggtccgttct gttcgactct
     2281 agggcgagcg tcactgaaac actgacgacg cctatcgcta ccgagcgttg ggcgaccgcg
     2341 tgcggcattc ttagctgcgg gcgcgagtgc atccgtcgtc ggcgctccaa ccagcgattg
     2401 acagttcgga acaagcggtt cagtctgggt ctgaaccaat caatccacgg cggaggcaag
     2461 cgatgccgag ggtgatattt gccggcctcc ccggcgcaga taccacatcg tccagcagcg
     2521 ttatggctcg ccggtgagtg tcgtcttaga cacagggcag atcccgaaag ccgaccgcgc
     2581 tgaggccgtg cgtgcggcgg tcgcagagca tttggtgccg gtcgagatcg attttgccgc
     2641 tgatcgcggc ccggcggcgg tgcctatggc gatcactgac ttggccgatc tcacggtttg
     2701 gacctcgacc tcgcatgcgg tgaaggtgca ccgcaccgcg gcacaaaccc acgacgactc
     2761 cacaccgagc atcttcatgg gtttgcaaat gacaggatcc tgtgtggtgg tccagcacgg
     2821 gcggcaagtg atcctgcggc gtggcgatct ggtcatctac gactcaacat caccgttcct
     2881 catttctgat gtcgacggga tccgccagca taaattccgg atacccctgg accgattggc
     2941 gttgccggcc gatgtcatcc ggcgggtctg tgcggtcagc ttgagtccgg ggcatccgat
     3001 caccgatctg gccgctacct atttgcaccg gctggcgctg cggccaggga tgttcgatcg
     3061 gccgggcggc gacctggtca gccagcccag cattgagtta ctgcgcgcgg taatcaccac
     3121 gcatctggac gccgtcgagt tgggtaagga gtcgctgcac gcaacgttgg tcgtgcggat
     3181 catggaatac gtccgcaccc acctgcgtga accccagctc agcgccggcc gcatcgccgc
     3241 cgagcaccac atatcggtgc gccagctcta ccgaatcctg gccgacgaag gcatttcgct
     3301 cggagactgg atccgcgcac ggcgactcga ggaatgccgc aaagacatcg cctcgacggg
     3361 cgcaggtgat cctgtcttcg tggttgcccg ccggtggggg ttcaccgacg cctccagttt
     3421 cgcgcggatg ttccgggcca cgttcggcat gtcgccgcgc gaatggcgtg agaccaaccg
     3481 ccgcccacct cactgaccgt cgcggccccc ataaggcgcg gtgcctcgtc gtgtgcccaa
     3541 catctgtgca ccgtgtgcca ggtcaggtta cggccgcacg ttttgcccac acaccgacct
     3601 agcgtcagag tcaacacctc cctactgcga ggtgtggtcg accaacgatg gggcaggtca
     3661 tgccggatcg cgaccgtcag ccgtctgagc cgaccggggc gtcgccgtgg gcggggtccg
     3721 cgccggccat accgcaactg agcgcggccc agttcgaccg catggcggcc tacggtgtgg
     3781 cgcgaccggt ggagttggga gacatcgtct ttggccccgg cgacgtcgac tacgacttga
     3841 tcctggtgga gtcggggtgg atcgagatcg tcagcccggt cgccggcgac gaaccagagt
     3901 cggtggtcgc ccggtacggc cccggggggg ttctgggcga actgaacttc ctgaccggcc
     3961 agaccgccta tctgatggcc cgcgtgatcg aggcaggccg catctaccgg atcacgcggg
     4021 cgcagttccg gcggttgatg gccaccgaac ccgatctgtc cgatatcttg ttgcgcacct
     4081 tgctggctcg gcgcgacctg ttgcgtgatg gaccagcggc ccgcgccatc gccatcctgg
     4141 gcagcgggtt gtcgggcgac agtctcgcgc tgcgcacgtt cgccgcccgt caacgcctgg
     4201 cctacctgtg gctggatgcc gacagtgccg caggccgggc gctcgccgaa tcggcgcgga
     4261 tcaccggcgc ggatctgccc gcggtgatca ccggccacca ggtgatgcgc cgcgccacca
     4321 ccgggcagat ggcccaggca ctgggcctgg cctaccggca caccgggacc aagccctcag
     4381 acctgatcgt ggtcggctcc gggcccgccg ggctgggcgc tgcggtttac ggcgcgtcgg
     4441 acgggctgga caccgtcgtc ctcgacgcgg ccggcgtggg cgggcaggcc gccgccagct
     4501 cccggatcga gaattatctc ggtttcccct ccgggctcag cggccaggag ctgacccaac
     4561 gcgcagccct gcaggcgatg aagttcggtg cccagctgtc cagcccgtgt cgggtggtcg
     4621 acctggacac cggcggagcc cacctgaccg tggtgctgag cgacggcacc accatcgagt
     4681 cccgcgccgt gctgatagcc accggagtgc actacaacac actgccgctg gagcgctgga
     4741 tcgagttcga aggagcggga atctattacg cggcaaccga actggaagcc cgcgcgtgcc
     4801 gtaccgagcc ggtgaccgtg gtgggtgggg cgaactccgc cggacaggcc gccctgttct
     4861 tggcgttgcg cggctgccag gtgactgtgg cggtgcgtgg gtcagacgtg gccgccacca
     4921 tgtcgtcgta tctgttgggg cggctgcgcg cagatccgcg cgtgacgatc cgagtcgaca
     4981 ccgaagtcac cggcctgtcc ggtgggtcgt cgctgcagcg catcaccctt accaacaatt
     5041 ccagcggtga ggtctcagaa cacctctgcg cgggcctgtt ctgcttcatc ggagcgcgac
     5101 cggaaaccga gtggctgcac gacgttgccg tcgacagtgc agggttcatc cgcacagacg
     5161 cccacctgga tcccgaggaa ctcggcacca cctggtcagc gctggggcgc ccaccactgc
     5221 cgtttgagac cagcatgccg accgtgttcg ccgccgggga cgtccgcttg tcgtcggtga
     5281 aacgcgtggc ctcggccgtc gaagagggcg caagcgcggt gcggtccatc cacaccgcgc
     5341 tcggaccaca cacctaaacg gccatcgccg gcgcgctcac cggcggcgca cgcacacatc
     5401 gcgacccact actacggcgc ggaacaacga cgcagaaccg ctctcccacc gggcagaccg
     5461 tgcgctgagc gccgcctaac cgagctggcg ttccaacgag aaactgctca ccgtgtgccg
     5521 agacctgtgc acggcgtgta tgagttgtcc gccaccggac cgactgtgcc cgaaccgatc
     5581 taacgtcgct gtgaaaggcc ttcccatcca tcgaggccgg ggcatatcca cgaagggaca
     5641 gctcatgcca cagcgcgacg cagctctcgc aatcgcaatt attgggccag ttcgtcggct
     5701 cgaattccgg aatggaaagt gcacctaacg cgttgttgaa cagatcggtc taaaaatcgt
     5761 gctgtcgatg gacggcacgc caccgaaagg tgagagatgc cgctgacaac tgagagcggt
     5821 cttgacggcg agtacgtgcc aagtccgatg gaaagcgtgc gcatgcaggt ggctggctat
     5881 gaagccagcg gcggtgtcaa gggcggaacg ctggagggtc ggccggtggt gatcctgacg
     5941 tcggtgggcg caaagtcggg caaggtccgc aaaaacccgg tgatgcggat cgtggacggc
     6001 gaccgctacg tggcggtggc atccgcgggc ggttcgccta ccaacccgtc ctggtacgcc
     6061 aatctagttg cccactcccg cgtgcacctt caggacggcg ccaatgtgag ggagttcgcc
     6121 gcccgcgagg tcgccggcga cgagaagcgc tattactggg cggtcgccga gcggttccgg
     6181 ccgcactttc ccgaatatcg acgcctggca ggcgaccgcg acatcccgat catggtgttg
     6241 gagcccatcg cgccgcgcac gccagtcacc ccgtttgtcc ggtacggcag catccctaac
     6301 ccctaccaag gaggcacggc catgacgaca ggagcagaag agcgcaacat agcggtcgtt
     6361 ctggaggcgt tcgacacgtt gttcaatcgg cgtgactacg ctgcggcgca gcggttctgg
     6421 tcacctgatt acatccagca cagcgcgcac atcgagccgg gacgtgaggg cctgttcgag
     6481 ctcgtgaagg cctcaccgcc agacatgcgc tacgagaacg ggctgatcgt cgccaacggc
     6541 gactatgtga tgctgcacgg ccgattcacc gacatcgggc aacccgccaa ctggatagcg
     6601 gcagacattg tgaggctcga aaacggtctg ctcgccgagc attgggatgt cctgcaggat
     6661 gaggccaccg ccgaggaatc cgtgagtggg ttgcccatgt tcggcgagac cttcccaaca
     6721 cgggtatgac acggtactgg ccgcctggga agcagaatgc gtcagtccca caacaattcc
     6781 cgcgcaagag cagcgttgca ggacaaccgg acgccgcata agcgcgtcca ccacgtgaga
     6841 cgaaattgga gggtcaactg atgtcgagac tggatggcaa gatcgctgtg attaccggcg
     6901 ggagcagcgg tattgggctg gcgaccgcac agcggttcgt ccaagaaggg gcgtacgtgt
     6961 ttattaccgg gcgccgccag agcgagttgg acaaggccaa agcactgatc ggcgacggcg
     7021 tcaccaccgt cgccgtcgac agcacgagca gtgcagacca ggacaccttg ttcgcgactg
     7081 tcctcgagga aaagggcgcg ctggacattc tcgtgacgaa ctcgggccgg gtggagcccg
     7141 aagagctcgg aaagatcacc gaggagaact tcgacctcac cttcgacctc aacgcccggg
     7201 caacgctgtt caccgtgcag aaggcggtgc cgctgatgcg tgacggcggc tcggtgattc
     7261 tgatcggctc ggtcgcgggc tacacgggca ttaacggcta caccacctac agcgccacca
     7321 aggctgcact gcgttcatat acccgcacgt ggacccggga gtccaaggac cgcggaatcc
     7381 ggttcaacac cttgagccca ggcccgatcg acacccccat catggacctt caggccgact
     7441 cgccggaggg cgcggacgcc atcagggcgg ccttcgcggc ggcgattccg ctcaaccgga
     7501 tggggcgccc ggaagagatc gcggccgcgg cactgtttct ggcctccgat gacagcagct
     7561 tcgtcgccgg cgccgaactg agcgtcgatg gcggtttcgc tcaggtctag ttcgggttaa
     7621 cgacatttta ggagtggtca tggcgagccc caccgcggtt cgggatctga caacgtgcaa
     7681 gccccctacc tcatcacgcc atcgccgtgg gccaccattt tcggcaccaa cgtggccgtc
     7741 gacggcggcc gagccggaac ctaacaccac cacaatcggg acacgacatg atgagcagga
     7801 ggaccaatga ctgcagtgtc accgaccccc gatcgggact acccccagct gcttggcgaa
     7861 cgaggcctca tcgagcctgc accgcgccgg gtacgcggct atttcgacaa gacactcgta
     7921 ttcgacaccg cacgcgccca ttacgtttgg gagatcgcgt actacccgca gtactacatt
     7981 ccgacacccg acgtgcgcat ggatcacttg cgtgacgaaa accacgtcca gaagctgcaa
     8041 ttcggttcct cgcggctgta ctcgctgaca tcggccgaac gcacctggcc ctctgccgcc
     8101 cgtgtctacg attccggtcc ggtcgcgggc agcgtgcggt tcgaatggga tgcgcttgac
     8161 tggttcgaag aggatgaacc actcatcctt catccacgca acccgtacgt gcgtgtcgac
     8221 tcgctgcgct cccaccgcca catcagggtc gagctcgacg gcgtggtact ggccgagacg
     8281 cacagcccgg tcttgctgtt cgaaacgggt ctgcctacgc gcttctacat cgaccgcacc
     8341 gacgtggagt tctcccagtt ggagccaagc aagaccgaat ccgtatgccc ctacaaggga
     8401 atcacctcgg cgtactggac attcagaaac ggcgacgccg tgcatccgga tatcgcctgg
     8461 gcctacgact acccgctgcg cgaagtcgcg cccatcgccg ggatgatcgc cttctacaac
     8521 gagaagctcg acatcttcgt cgacggcgcg ttgttgtctc gccctcacac catcttcacg
     8581 tagcggctca ggggaagatg cgcagctcgc gaaagtctgg tgagctgccg cctgaagctc
     8641 tgatgcgtgt cgatccaatc cagcgcgcac tacttcctcg gagcggcact cgcccgatgg
     8701 tgacaatcaa tgcgttcctg ggacgtaatg ctctgccgcg ttcgcgatgt cgattattcc
     8761 gcccgggtcg cccgaaacac cagcccgtac tcgtcgagtg cgactacggc actgtcggtc
     8821 gccgcgacgg cgtccacccg gatcgctgcg gcaggactac ggctctttac caacgcgccg
     8881 atgtcgcgcc aaatggtttg gctgagttgt cttccagacc ggtgatcgac cagcagggcc
     8941 gcactgacga acccgtcggt ttccgtcagc caaggcaccg ctgtgtcttc atatccggcg
     9001 atcgcctggt cgagtgcggc cgggttcgtc tgatagacgg tgtggcgcac gcccgcgccg
     9061 ccctcttctc gccccacccg agtcacgctc gccctccgaa agcgttcgac cgaggcggtg
     9121 ccgagcgcaa gacggaccgc ttcgtcgcgg acaccggcct ctacgcgttc gctttctcgc
     9181 atggcgtcgt gtgagaccca gaacgactcg aggaccgcga cggccaggtc gggagatacc
     9241 tgcagcgaca ttccttgctc gccaggttct gactccaggc gcgatcggcc atcagaagcg
     9301 aggtatgcga cggcgtcgtc aatccgttcc gggtcggcgg ttatcagcgt gtaccgaaca
     9361 tgcatctgtg gctccttcac tcgtcacgac ggcggggact gtgccgccat aaggacggca
     9421 agcgtgcgtt cggccgcatg cttcgaggac gccggattct ggccggtgac aaggtgcccg
     9481 tccaccacca cgtgcgaact ccagggttgc cccgcgtcga agtccgctcc ctcctcgcgc
     9541 agtcgggtct ccagtagcca cttggcacgg tctgccagac ccgcttggcg ctcttcctca
     9601 ttcgtgaagg cggtcagccg tcggccggcg aacagccagg tgccgtcctc gcggcgggcc
     9661 gccagcagtc cggccggccc gtggcatacc gcagcgacga ctcgtcccgt gtcgaacaga
     9721 tcggccagaa ttcggccgag cggtcgacag tccggcagat cttccatcgg cccgtggccg
     9781 cccggaatga agacggcgtc gtagttgccc aggttgtcgg cggcgtgctc caccgacatt
     9841 ggggcggcga gctcgtgctc cagcgat
//
LOCUS       DASYLX010000576         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2113291,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000576 DASYLX010000000
VERSION     DASYLX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113291"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VGP27_13880"
     CDS             <1..313
                     /locus_tag="VGP27_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852422.1"
                     /translation="VNRYRRQGQHCPLAVLMSEIGRTTPGAQAVTSELMHKWHTEIAD
                     GVRHMQGQGKVAGGLDAGRIAAALLAGIQGGVGVLLATGDLSYLEAALDVGIESLRSA
                     D"
     gene            complement(382..1644)
                     /locus_tag="VGP27_13885"
     CDS             complement(382..1644)
                     /locus_tag="VGP27_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1298 domain-containing protein"
                     /protein_id="HEV7852423.1"
                     /translation="MTVRRLAAVDAQTYLLSAKIPNDPLLLYGFAGAPANLEQAIEVV
                     RCRANDCAELRLKIRAGNIFTYPAWVSGDVDADRFTVHELDDNSWVGCLAAVAKLADD
                     HLDPRVRPWRLHFFTPVEGIPVARGAGTVAVLQISHACADGARSSALAAHLFGRAGDI
                     APVATPRLRGVKLPWRTLVAARTHRQLVRDTEAALVPAQAKSRPPLRTNAPPAGKRSV
                     RTLIRRRNQLPGPTVTIGVLCAVSTALSAHLRELGDDPSTLGAEVPMAKTTAREAHNH
                     FGNVGVGLYPELGLNERATRIAGDIADRRRRAEHPAMRAASRAFAVTPAPLLRWGAEQ
                     FDPAVRSSTVIGNTVVSSVNRGPTDLSFGEVPAVVTAGYPALSPMMGLTHGVHGIGDT
                     VAVSVYAAESAIGDIDEYVARLDAALSG"
     gene            complement(1655..>1856)
                     /locus_tag="VGP27_13890"
     CDS             complement(1655..>1856)
                     /locus_tag="VGP27_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7852424.1"
                     /translation="LKPPLMPFLYLHGDDDGCMAPDYIAWVERILPSGSDASVIEGAG
                     HFLQLEQPDVVARHIVDFIGRA"
BASE COUNT          309 a          620 c          632 g          295 t
ORIGIN      
        1 ggtgaatcgc taccggcggc aggggcagca ctgcccgctg gcggtcttga tgtcagagat
       61 cggccgaacc acccccggtg cgcaggcagt gacctcggag ctgatgcaca agtggcacac
      121 cgagatcgcc gacggcgtgc gccatatgca gggacaaggc aaagtcgccg gcgggctcga
      181 tgccgggcgg atcgccgccg ccctgctcgc cgggatccag ggcggtgtcg gcgtgttgct
      241 ggcgacgggc gacctcagtt acctcgaagc ggccctggac gtcgggatcg agtcccttcg
      301 ttccgcggac tgaattcgcc actgtcgatc gccgccttta actacgtagt cggcgtcaaa
      361 agggcggatc ggacacggca ctcatcctga cagtgcggcg tcgagccggg cgacgtattc
      421 gtcgatgtca ccgatcgcag actcggcggc atacacgctg acggcgacgg tgtccccgat
      481 gccgtgcacg ccgtgggtga ggcccatcat cggcgacagc gcggggtagc cggcggtgac
      541 gaccgcgggc acttctccga agctcaggtc ggtaggtccc cggttgacac tggagacgac
      601 cgtgttgccg atgaccgtcg acgaccgcac ggccgggtcg aactgctcgg cgccccaacg
      661 tagcagcggt gcgggcgtga ccgcgaacgc ccggctggca gcacgcatcg ccggatgctc
      721 ggcccgccga cggcggtcgg cgatgtcgcc ggcgatccgc gtcgcgcgct cgttgagacc
      781 cagctccggg taaaggccga cgccgacatt gccgaagtgg ttatgcgcct cacgtgcggt
      841 ggttttcgcc atcggcacct cggcgccaag agtcgacggg tcgtcgccca gttcgcgcag
      901 atgagcggac aacgccgtgg acaccgcgca caacacaccg atcgtcacgg tcggaccggg
      961 gagttggttc cgtcgccgta tcagggtgcg cacgcttcgc tttccggccg gtggggcatt
     1021 ggtccgcagc ggtggacgcg acttcgcctg cgcgggcacc agagccgctt cggtgtcgcg
     1081 gaccagctgt cggtgtgtcc gtgcagcgac caaggtgcgc cacggcagtt tgacaccgcg
     1141 cagtcgcggc gtcgccaccg gcgcgatgtc gcccgcgcgc ccgaacaggt gcgccgccaa
     1201 cgccgatgac ctcgccccgt cggcgcaggc atgcgagatc tgcaggaccg ccacagtccc
     1261 agcgccgcgg gcgacgggta tgccttccac cggggtgaag aaatgtagcc gccatggccg
     1321 cacccgcgga tcgagatggt cgtcggcgag tttggcgacc gccgccaggc accccaccca
     1381 gctgttgtcg tcgagttcat gcaccgtgaa ccggtcggca tccacgtcgc cgctcaccca
     1441 cgccggatag gtgaagatgt tgccggcgcg aatcttcagc cgaagctccg cgcagtcatt
     1501 ggcccggcag cgaacgactt cgattgcctg ctcgagattg gctggggctc cggcgaaccc
     1561 gtacagcagg agcgggtcgt tgggaatctt cgccgacaac aagtaggtct gcgcatcgac
     1621 cgccgctagc cggcgcaccg tcatctggtt cccgtcaggc tcgcccgatg aagtcgacga
     1681 tgtgtcgcgc caccacatcc ggctgctcga gttggaggaa atgacccgca ccttcgatca
     1741 cagaggcgtc acttcctgac ggaagtatcc gctcgaccca ggcgatgtaa tccggtgcca
     1801 tgcagccgtc gtcgtcgccg tgcagataca gaaagggcat caaaggcggc ttcagg
//
LOCUS       DASYLX010000577         4721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3538408,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000577 DASYLX010000000
VERSION     DASYLX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3538408"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2451
                     /locus_tag="VGP27_13895"
     CDS             196..2451
                     /locus_tag="VGP27_13895"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV7852425.1"
                     /translation="MSVAELEDGVFESTAVIDNGSFGKRTIRFETGRLAKQAAGSVVA
                     YLDDETMLLSATTASKTPKEHFDFFPLTIDVEERMYAAGRIPGSFFRREGRPSTDAIL
                     TCRLIDRPLRPTFVSGLRNEIQVVVTVMSLDPKDLYDVLAINAASASTQISGLPFSGP
                     VGGVRVALIDGTWVAFPTVEQLEGAVFDMVVAGRKTADDVAIMMVEAEATDKVIELIA
                     GGAGAPTETIVAEGLEAAKPFIATLCAAQQELADAAAKPVADYPLFPEYGDDVYYAVA
                     SVATDELSKALTIASKTERNERTDEIKAEVLERLGEQYAGREKEIGAAFRALTKKLVR
                     QRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQQ
                     IDSLGPETSKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFP
                     YAIRQVSEALSSNGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDDVELEDGST
                     ERRFVALTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSSVLAGALAQAKDA
                     RNTILEVMAEAIDEPDEMSPYAPRVTAIKVPIDKIGEVIGPKGKMINSITEETGAQIS
                     IEDDGTVFVGATDGPSAQAAIDRINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLPG
                     RDGLVHISKLGRGKRIAKVEDVVKVGDKLRVEIADIDNRGKISLVLVAEEDSAEDAPS
                     ADSPVDATTGS"
     gene            2432..3784
                     /locus_tag="VGP27_13900"
     CDS             2432..3784
                     /locus_tag="VGP27_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV7852426.1"
                     /translation="MPRQAASGKAGLRRVRRGDESTTKSTLRRTTLPGGLRVVTEYVP
                     SVRSASVGVWVGVGSRDEGPSVAGAAHFLEHLLFKATPTRSAVEIAQAVDAVGGELNA
                     FTAREHTCYYAHVLDNDLELAVDLVADVVLRGTCAAEDVELERAVVLEEIAMRDDDPE
                     DTLGDVFLSAVFGEHPVGRPVIGSAASVSAMTRSQLHSFHVRRYTPDRMVVAVAGNVE
                     HDEVVRLVREHFGRHLVRDRTAVPPRKGTGRVTGQPSLEVVNRDAEQTHMSLGVRVPG
                     RHWKRRWALSVLNTALGGGLSSRLFQQIRETRGLAYSVYSTVDTFSDSGALSVYAACL
                     PERFAEVARVTADVLETVARDGITESECRIAKGSMRGGLVLGLEDSGSRMNRIGRSEL
                     NYGEHRSIDETLALIDAVTLDEVNAVAHQLLTRPYGAAVLGPYRTKRSLPQPLRAITG
                     "
     gene            3802..4707
                     /gene="bla"
                     /locus_tag="VGP27_13905"
     CDS             3802..4707
                     /gene="bla"
                     /locus_tag="VGP27_13905"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEV7852427.1"
                     /translation="MTDLSRRSLLAGGLAVAGLATFPQKPVFGDTQGEPVPPVHDRIE
                     ALQRRHNTKIGVYAVDFETGRTVSHLDGESFAMCSTFKGYAAARVLQMVQNGELTLDR
                     QVFVDPAQAALPNSPRTVPNSGGQMTLAELCAAAVQVSDNAAGNLLLQTIGGPSVITD
                     FARSIGDDRTRLDRWEIELNSAIPGDPRDTSSPQALGGGYRTLLTGDVLAPPQRQQLE
                     DWMRANETSSVRAGLPEGWTTADKTGNGDYGSTNDVGIAYGPDGRRLLLAIMTRSQAN
                     DPKAPNNRPLIGEVTSLLVPWLLGA"
BASE COUNT          824 a         1576 c         1587 g          734 t
ORIGIN      
        1 ctggcggacc ccgcctgatc gggcggtctt cggtagtggc ggccgggagc cccaagaaat
       61 tgggttgagc ctccggccgc ttcgatcgac ggccgtagcc gtatcaaggc ccgtggtctg
      121 acaggctgct cgacgtattg agcgtgacga cgcgaaacag ctgaacagag acccagagag
      181 gccatacgga cacacatgtc tgttgcagaa cttgaggacg gcgtcttcga atcgaccgcc
      241 gtcatcgaca acggaagctt cggcaagcgc accatccgat tcgagaccgg ccgccttgcc
      301 aagcaagcgg cgggctccgt cgtcgcctac cttgacgacg aaaccatgct gctgtcggcg
      361 accaccgcca gcaagacccc caaagaacat ttcgacttct tccctctgac gatcgacgtc
      421 gaggaacgca tgtacgccgc cgggcgcatt cccggctcgt tcttccgcag ggaaggccgt
      481 ccgtcgacgg acgcgattct cacctgccgc ttgatcgacc ggccgctgcg cccgacgttc
      541 gtctccggcc tgcgcaacga gatccaggtc gtcgtcaccg tgatgagcct tgaccccaag
      601 gatctctacg acgtgctggc catcaacgcc gcgtcggcgt cgacccagat ttcgggcctg
      661 ccgttctccg gtcccgtcgg tggtgtgcga gtcgcgctta tcgacggcac ctgggtggca
      721 ttcccgaccg tcgagcaact cgaaggcgcg gtattcgaca tggtcgtcgc cggccgcaag
      781 acagccgacg acgtcgcgat catgatggtc gaggccgaag ccaccgacaa ggtgatcgag
      841 ctgatcgccg gcggagccgg cgccccgacc gagaccatcg tggccgaggg cctcgaagcg
      901 gccaagccgt tcatcgccac gttgtgcgcc gcccagcagg aactcgccga cgcggccgcc
      961 aagccggtcg ccgactaccc gctcttccct gagtacggcg acgacgtgta ctacgcggtc
     1021 gcgtcggtgg ccaccgacga actgtcgaag gcgctcacca tcgcgagcaa gaccgaacgc
     1081 aacgaacgca ccgacgagat caaggccgag gtgctcgagc ggctcggcga gcagtacgcg
     1141 ggccgcgaga aggagatcgg tgccgcattc cgggcactga ccaagaagct tgtccgtcag
     1201 cgcatcctta ccgaccactt ccgcatcgac gggcgcggta tcaccgatat ccgtgcgctt
     1261 tccgccgagg tggccgtcgt tccgcgggcg cacggcagcg cgctgttcga gcgcggtgag
     1321 acccagatcc tcggtgtcac cacgctggac atggtgaaga tggcccagca gatcgactcg
     1381 ctgggaccgg agaccagcaa gcgctacatg caccactaca acttcccgcc gtactcgacc
     1441 ggtgagaccg gtcgagtcgg ctcgccgaag cgccgcgaga tcgggcacgg cgccctggct
     1501 gagcgggcac tcgtgccggt tctgccgagc gtcgaggaat tcccgtacgc gattcgccag
     1561 gtttccgagg cgctcagctc gaacggctcg acgtcgatgg gctcggtgtg tgcatcgaca
     1621 ctgtcgctgc tgaacgcggg tgtgccgctg aaggcgcccg tcgcaggcat cgccatgggc
     1681 ctggtgtccg acgacgtcga actggaggac ggcagcaccg agcgtcgctt cgtggcgctc
     1741 accgacatcc tcggcgccga ggatgctttc ggggacatgg acttcaaggt ggcaggcacc
     1801 aaggacttcg tcaccgcgct gcagctggac accaagctcg acggcatccc gtcgtctgtc
     1861 cttgccggag cgttggcaca ggccaaggat gcgcgtaaca ccatcctcga ggtgatggcc
     1921 gaggcgatcg acgagcccga cgagatgagc ccctacgcgc cacgtgtcac cgcgatcaag
     1981 gtgccgatcg acaagatcgg cgaggtcatc ggacccaagg gcaagatgat caattcgatc
     2041 accgaggaga ccggtgccca gatctcgatc gaggacgacg gcaccgtgtt cgtcggcgcg
     2101 accgacggac cttcggcgca ggccgcgatc gaccggatca acgcgatcgc caacccgcag
     2161 ctgcccaaga tcggcgagcg gttcctcgga actgtggtca agacaaccga tttcggcgcg
     2221 ttcgtgtcgc tgctgcctgg ccgtgacggt ctggtgcaca tctccaagct cgggcggggc
     2281 aagcgcatcg ccaaggtcga ggatgtggtg aaggtcggcg acaagctgcg tgtcgagatc
     2341 gccgacatcg acaatcgcgg caagatctcg ctcgtcctcg tcgccgaaga agactcagcc
     2401 gaggacgccc cgtccgctga ttcgcctgtc gatgccacga caggcagcta gcggcaaggc
     2461 gggcctgcga cgggtccgcc gcggcgacga gtccaccacc aagtccacac tgcgacgcac
     2521 caccttgccc ggtgggctgc gcgtcgtcac cgaatacgtc ccgtcggtgc gctcggcgtc
     2581 cgtcggcgta tgggtgggcg tcggttcgcg cgacgaaggg cccagcgtcg ctggcgccgc
     2641 acactttctc gagcacctgt tgttcaaggc caccccgacc cgaagtgcgg tggagatcgc
     2701 ccaggcggtg gacgcggtcg gcggcgagct gaacgcgttc acggccaggg aacacacctg
     2761 ctactacgcc cacgtcctcg acaacgacct ggaactggcg gtcgacctgg tcgccgacgt
     2821 ggtgctgcgg gggacatgtg cggccgagga cgtcgagctc gaacgtgctg tggtgctcga
     2881 ggaaatcgcc atgcgcgacg acgaccccga ggacacgctc ggcgacgtct tcctgtcggc
     2941 ggtcttcggg gaacacccgg tcgggcgtcc cgtgatcggc agcgcggcgt cggtttccgc
     3001 gatgacgcga tcgcagctgc attcgttcca cgtccgccgc tacacgcccg atcggatggt
     3061 ggtggcggtc gcgggcaatg tcgagcacga cgaggtggtc cggctggtgc gtgagcactt
     3121 cggccggcac ctggtgcgcg atcgcacggc ggtgccgccc cgcaagggca cgggccgcgt
     3181 caccggtcag ccgtctcttg aggtggtcaa ccgggacgcc gagcagactc acatgtcgct
     3241 gggggtgcga gtgccgggca ggcactggaa gcgccgctgg gcgttgtcgg tactcaacac
     3301 cgccttgggc ggagggttga gttcgcgcct gttccaacag attcgcgaaa cacgcgggct
     3361 ggcgtactcg gtgtactcga cggtggacac cttctccgac agcggcgcgc tgtcggtcta
     3421 cgccgcgtgt cttcccgagc gcttcgccga agtggccagg gtgaccgccg acgtgctgga
     3481 gacggtggcc cgcgacggga tcaccgaatc cgagtgccgg atcgccaagg gctcgatgcg
     3541 cggtgggctg gtgctgggcc tggaggattc cggttcgcgg atgaaccgca tcggccgcag
     3601 cgaactgaac tacggcgagc accgcagcat cgacgagacg ctggcgctga tcgacgcggt
     3661 caccctcgat gaggtcaacg cggtcgcgca tcaactgctg accaggccgt acggtgccgc
     3721 ggtgctgggg ccgtaccgca ccaaaaggtc actgccacag ccgcttcggg ccatcacagg
     3781 ctgaactgta acgtggggac ggtgactgac ctctcccggc gaagcctgct ggccggcgga
     3841 ttggccgtgg caggcctcgc cacctttccg caaaaacctg tctttggcga cacacaaggg
     3901 gagccggtcc cgccggtgca cgaccgtatc gaggcgctgc agcggcggca caacacgaag
     3961 atcggcgtgt acgcagtcga cttcgaaacc ggccgcacgg tgtcgcacct tgacggcgag
     4021 tcgttcgcga tgtgctcgac gttcaagggt tacgccgccg cgcgcgtcct gcagatggtg
     4081 cagaacggtg agctgacgct ggaccgccag gtcttcgtcg accccgccca ggccgcgttg
     4141 ccgaactcgc cgaggaccgt cccgaactcc ggcggtcaga tgacgctggc cgagctgtgc
     4201 gcggcggccg tgcaggtcag tgacaacgcc gcgggcaatt tgctgctcca gacgatcggc
     4261 ggcccctcgg tcatcaccga tttcgcgcgc agcatcggcg acgaccgcac ccggcttgac
     4321 cgctgggaga tcgagttgaa ctcggcgata ccgggcgacc cgcgggacac cagctcgccg
     4381 caggcgctcg gtggcggcta ccgcaccctg ctcaccggcg acgtgctggc cccgccgcag
     4441 cgccagcagc tcgaggactg gatgcgggcc aacgaaacct cgagcgtgcg cgcaggtctg
     4501 cccgagggct ggaccaccgc ggacaagaca ggcaacggcg actacggcag caccaacgac
     4561 gtcggtatcg cgtacgggcc cgacggcagg cgcttgctgc tggccatcat gacgcgctcg
     4621 caggccaacg acccgaaggc accgaacaac cggccgctga tcggcgaggt cacctcgctg
     4681 ctggtgccgt ggctgctcgg cgcttgagcg ggaagcgatg a
//
LOCUS       DASYLX010000578         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1125143,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000578 DASYLX010000000
VERSION     DASYLX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125143"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VGP27_13910"
     CDS             <1..1264
                     /locus_tag="VGP27_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEV7852428.1"
                     /translation="YTEASLIKSLEDLGIGRPSTYSSIIKTIQDRGYVHKKGSALVPS
                     WVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIASGHEQRTNWLNNFYFGGDHGVADS
                     IARSGGLKKLVGGNLEEIDAREVNSIKLFDDAQGTSIYVRVGRNGPYLERTVIGEDGE
                     PTPQRANLNDSLTPDELTLELAEKLFSTPQEGRSLGVDPVTGHEILARDGRYGPYVTE
                     VLPEPEDPEDGAAAKKGKKPTGPKPRTGSLLRSMDLETVTLEDALRLLSLPRVVGADP
                     STGEEITAQNGRYGPYLKRGTDSRSLTTEEQMFDITLDEALKIYSEPKRRAGQGASAP
                     PLRELGADPATGKPMVIKDGRFGPYVTDGETNASLRKGDDVLSITDARAGELLADRRA
                     RGPVKRTAKKTAKKAAKKAPAKKAAKKA"
     gene            complement(1272..2879)
                     /locus_tag="VGP27_13915"
     CDS             complement(1272..2879)
                     /locus_tag="VGP27_13915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7852429.1"
                     /translation="MTTQAPPIGRIRAFVRWVARTPWPVFTLGMLQSDIIGALFVLGF
                     LRFGLPPEDRIQLQDLPAFNLAVFLGFLFLSFTVGAYVSLKLLIPVMRWQRRDSLLVD
                     SDPAATELARTRALKMPFYRSLISVVNWCLGSIVFIVASWPVASHAAPVVAVATALGA
                     TATAIIGYLQSERVLRPVAVAALRGGVPENYRAAGVILRQVLTWVLSTGVPVLAIVLA
                     LVASKFQILTASAERLTTPILIMAIAALLVGLAGTVLVAMAIADPLRQLRWALGEVQR
                     GNYNAHMQIYDASELGLLQAGFNDMVRDLAERQRLRDLFGRYVGEDVARRALERGTEL
                     GGQERDVAVLFVDLVGSTQLASNRPASEVVSLLNEFFRVVVDTVARHGGFVNKFQGDA
                     ALAIFGAPIEHPDASGAALAASRELHDELLNVLGDELEFGIGVSAGRAIAGHIGAQAR
                     FEYTVIGDPVNEAARLTELAKLEEGHVLASAIAVSGALDAEALCWDVGEIVELRGRTA
                     PTQLARPLNLVEIRQTEPEEMSSEASR"
BASE COUNT          570 a          960 c         1052 g          449 t
ORIGIN      
        1 gtacaccgag gcgtcgctga tcaagtcgct ggaggatctt ggcatcggcc ggccgtcgac
       61 gtactcgtcg atcatcaaga ccatccagga ccgcggctac gtccacaaga agggcagcgc
      121 gctggtgccg tcgtgggtgg ccttcgccgt caccggtctg ctcgaacagc acttcgggcg
      181 gctggtcgac tacgatttca ccgccgcgat ggaagacgaa ctcgacgaga tcgcttcagg
      241 ccacgagcag cgcaccaact ggctcaacaa cttctacttc ggcggcgacc acggggtggc
      301 tgattcgatc gcccgctccg gcggcttgaa gaagctcgtc ggcggcaatc tcgaggagat
      361 cgacgcacga gaagtcaact ccatcaagct gtttgacgat gcgcagggaa cctcgatcta
      421 cgtccgtgtc ggcaggaacg gaccttatct ggagcgcacc gtcatcggcg aggacggcga
      481 acccacaccg cagcgggcca atctcaacga ctcgctgaca ccggatgagt tgacgctgga
      541 gttggccgag aagctgttct ccacaccgca ggaaggtcgg tcgctgggtg tagacccagt
      601 aacgggccac gagatcctgg ccagggacgg acgctacggg ccgtatgtga ccgaggtgct
      661 tcccgagcca gaagaccccg aagacggcgc ggctgccaag aagggcaaga agccgacggg
      721 ccccaagccg cgcaccggtt cacttctgcg gtcgatggat ctggagacgg tcacgctcga
      781 ggacgcgctg cgactgctgt cgctgccacg cgtcgtcggc gcagacccga gcaccggcga
      841 ggagatcacc gcgcagaacg ggcgctacgg gccttacctg aagcgcggca ccgactctcg
      901 ttcgctgacc accgaagagc agatgttcga catcaccctc gatgaggcgt tgaagatcta
      961 ctccgagccc aaacgccgtg cgggacaagg tgcctcagcg ccgccgcttc gggagttggg
     1021 tgcggatccg gcgacgggta agccgatggt catcaaggac ggccgtttcg gtccgtatgt
     1081 gaccgacggc gagaccaacg ccagcctgcg caagggcgac gacgtgctgt cgatcaccga
     1141 cgcgcgggcc ggcgagctgc tggccgaccg gcgcgcacgt ggtccggtca agcggacggc
     1201 gaaaaagacc gccaaaaagg cggcgaagaa ggcgcccgcg aagaaggctg ccaagaaggc
     1261 ctagccgttc gctagcggga agcttcgctc gacatctctt ccggctctgt ctggcggatc
     1321 tcgacgaggt tcagcggacg ggcgagctgg gtcggtgcgg tacggccgcg gagttcgacg
     1381 atctcgccga cgtcccagca cagcgcctcg gcgtccagcg ctccgctgac cgcgatcgcc
     1441 gatgccagca cgtggccctc ctcgagcttc gcgagctcgg tgaggcgggc ggcttcgttg
     1501 accgggtcgc cgatcacggt gtactcgaag cgggcctgag cgccgatgtg gccggcgatg
     1561 gcgcggcccg cggacacccc gatgccgaac tccagctcgt cgcccagcac gttcagaagc
     1621 tcgtcgtgca gctcgcgaga ggccgccagc gcggcaccgg acgcgtccgg atgctcgatc
     1681 ggcgcgccga agatggccag tgcggcatcg ccctggaatt tgttgacgaa gccgccgtgg
     1741 cgagcgacgg tgtcgacgac gacgcggaag aactcgttga gcaggctgac gacctcggag
     1801 gcgggccgat tggaggccag ctgcgtcgag ccgaccaggt cgacgaacag cacggcgacg
     1861 tcgcgttcct gaccgccgag ttccgtgccg cgttcgaggg cgcggcgggc gacgtcctcg
     1921 ccgacatagc gaccgaaaag gtcgcgcagc ctctgccgtt cggcgaggtc gcgaaccatg
     1981 tcgttgaatc cggcctgcag caagcccagt tcgctggcgt cgtagatctg catgtgcgcg
     2041 ttgtagttgc cgcgctggac ttcaccgagc gcccagcgca attggcgcag cggatcggcg
     2101 atggccatcg ccaccagcac ggtgccggcc aaaccgacga gcagtgcggc gatggccatg
     2161 atcaggatcg gcgtggtcag ccgctcggcg gacgcggtga ggatctggaa cttgctggcc
     2221 accagtgcca gcacgatcgc cagcaccggg acgccggtgg agagcaccca cgtcagtacc
     2281 tgccgaagga tgacgcccgc ggcgcggtag ttctccggta caccgccacg tagcgccgca
     2341 acagcgacag gccgcagcac ccgttcggat tgcaggtagc cgatgatcgc cgttgcggtc
     2401 gcgccaagcg cggtggcgac cgcgacaaca ggtgctgcat ggctggctac cggccaactg
     2461 gcgacgatga agacgatgga ccccaggcac cagttgacga cgctgatgag cgagcgatag
     2521 aacggcatct tgagcgcgcg ggtgcgggcc agttcggtgg cggccgggtc gctgtcgacg
     2581 agcaggctgt cgcgccgctg ccagcgcatc accgggatca gcagcttcag gctgacgtag
     2641 gcgccgaccg tgaacgacag gaacaggaag cccaggaaaa cggccaggtt gaaggcgggc
     2701 aggtcctgaa gctggatgcg gtcctcggga ggtagcccga accggagaaa gcccagcacg
     2761 aacagcgcgc cgatgatgtc ggactgcagc atgcccagcg tgaacaccgg ccatggcgta
     2821 cgcgccaccc agcgaacgaa tgccctgatt cgtccaatcg gtggggcctg cgtggtcacc
     2881 cgttaaccgt atcgggccgg ggagaccgga atggacgcac tgggacggac gtgtcggttc
     2941 gcagcactac tgttacctct gatggccggt gtgttttcgc gtctggtggg acagggcgcc
     3001 gttgaggcgg aattggtggc cgcagcacag g
//
LOCUS       DASYLX010000579         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1344258,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000579 DASYLX010000000
VERSION     DASYLX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344258"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VGP27_13920"
     CDS             complement(<1..1502)
                     /locus_tag="VGP27_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7852430.1"
                     /translation="MTTPDSDAFAAQTTFADLQIHPAVLQAVADVGYESPSPIQAATI
                     PAMIAGSDVVGLAQTGTGKTAAFAIPILSKIDTSSRITQALVLAPTRELALQVAEAFS
                     RYGARLAVNVLPIYGGASYGPQLAGLKRGAQVVVGTPGRVIDHLEKGTLDLSHLDYLV
                     LDEADEMLQMGFAEDVERILEGTPEYKQVALFSATMPPAIRKITNKYLHDPVQVKVES
                     KTATADNISQRYIQVSGHRKMDALTRLLEVEPFEAMIVFVRTKQATEEVADRLKARGF
                     AAAAINGDIPQAQRERTIASLKDGKLDILIATDVAARGLDVDRISHVLNYDIPHDPES
                     YVHRIGRTGRAGRSGAALLFVTPRERHLLNSIERVTRQKLVESRLPSVDDVNAQRVEK
                     FRDSITTALNAPGIDLFRKLIEGYERDHDVSMADIAAALALQSRDGEEFLMTEPPPEK
                     RRERPDRDQREDGPPRKRRDVRTDLATYRIAVGKRHKVMPGAIVGAIANEG"
     gene            1627..2178
                     /locus_tag="VGP27_13925"
     CDS             1627..2178
                     /locus_tag="VGP27_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HEV7852431.1"
                     /translation="MAATAAAAGVLLLAGCGSGDSTVSKTPNASTTTAAPAPATAQPA
                     PPRPSAAPSTVAPDPCAVNLAAPEIAKAVSELPRDPRSGQAWSPEPLAGTYNECAQLS
                     AIIVKANTNAENPNTRAVLFHLGKFIPTGVPDTYGFNSVQPSESTGDTVALVYSNGTP
                     GLDSVVKFRWNGAGVELIGNTPG"
     gene            2294..5704
                     /locus_tag="VGP27_13930"
     CDS             2294..5704
                     /locus_tag="VGP27_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEV7852432.1"
                     /translation="MFVADDRVIYSASDLAAAARCEYALLRSFDARLGWGPPVAAEDE
                     LLARTAKLGGEHEQRHLDELRSATEGTVAVIGRPDYTVAGLEAAAEATLRAVRRRAPV
                     IYQAAMFDGRFVGFADFLVFDGERYRLRDTKLARSVKVEALLQLAAYAETLAAAGVPV
                     SDEVELVLGDTATVGYRVDELVPVYRPRRAELERLLDGHLAGGRAVRWSDEHVRACFR
                     CPECTDQVREHDDLLLVAGMRASQRARFIDAGIATETDLARHDGAVPELSARTVATLS
                     AQARLQLTPRIDGKPPYEIADAQPLMVLPEPDKGDLFFDFEGDPLWTDDGREWGLEYL
                     FGVLDTADDFKPLWAHNRTDERQALRDFLKMVRHRRRRFPHMHIYHYAAYEKTALLRL
                     AGRYGEGEDEVDDLLRNGVLVDLYPLVRKSVRVGTENYSLKSLEPLYMGGQLRSGDVT
                     TATDSITQYAKYCELQAAGRDDDAAIVLKEIEDYNRYDCTSTRKLRDWLICRAIERAV
                     PPVGAQPVADSAPIADDDTLARTLAKFVGDEPAGRSPDQTAVAMIAAARGYHRREDKP
                     YWWGHFDRLNNPVDEWGDNSDVFIAEKAVVESDWHTPPKARKPQRWVRLYGGIATGEL
                     SKSMYALYEPPSPAGLTDDTERRAFGSVTVTECDDPAAPTEVLVVEREPRKGGPFDQC
                     PFALTPGPPISTAPLRDSIEATATEIAAGLPQLPPSAVIDILLRCAPRTGSGAPLPRN
                     PVAAESITAALLDLDSSYVAVHGPPGTGKTFTAAAVIANLVKHSGWRIGVVAQSHAVV
                     ENLFRDVIAAGVDPALVAKKKHGTDACWHEIDDKEYPAFIADHDGCVIGGTAWDFANP
                     GRVPRNSLDLLVIEEAGQYSLANTIAVASAARNLLLLGDPQQLPQVSQGTHPEPVDTS
                     ALGWLVDGRHTLPAELGYFLDVSYRMHPAVCAAVSRLSYEGRLRSFDGVTAARTLAGQ
                     APGVRVLTVDHDGNSTDSPEEADAIVAEVKRLLGSAWTDEHGTRPLKQDDVLVVTPYN
                     AQVVLLRQRFDAAGLADVRAGTVDKFQGRQAPVVFVSMTASSIDDVPRGIAFLLNRNR
                     LNVAISRAKYASIIVRSETLTEYLPTTPDRLIELGAFLSLPSCDKPTE"
BASE COUNT          915 a         1956 c         1983 g          904 t
ORIGIN      
        1 ccttcgttgg cgatcgcacc cacgatggca cctggcatca ccttgtgccg cttgccgacc
       61 gcgatgcgat acgtggcgag gtcggtgcgg acgtcccgcc gcttgcgcgg cggcccgtcc
      121 tcgcgctggt ctcggtcggg gcgctcgcgg cgcttctccg gcggcggttc ggtcatcagg
      181 aactcttcgc cgtcgcggct ctgcagggcc agcgcggcgg cgatgtcggc catcgacacg
      241 tcgtggtcgc gttcgtagcc ctcgatcagc ttgcggaaca ggtcgattcc cggcgcgttg
      301 agcgcggtgg tgatcgagtc ccggaacttc tccacccgct gggcgttcac gtcgtcgacc
      361 gacggcagcc gcgactcgac gagcttctga cgggtgaccc gctcgatcga gttgagcagg
      421 tgccgttcgc gtggcgtgac gaacagcagc gccgcgcccg accgcccggc ccggccggtg
      481 cgcccgatcc ggtgcacata ggactccgga tcgtgcggga tgtcgtagtt cagcacgtgt
      541 gagatgcgat cgacgtcgag cccgcgggcc gctacgtcgg tcgcaatcaa gatgtcgagc
      601 tttccgtcct tgagtgaggc gatggtgcgc tcgcgttgag cctgcgggat gtcgccgttt
      661 atggccgccg ccgcgaaacc gcgcgccttg agtcggtcgg cgacctcctc ggtggcctgc
      721 ttggtgcgga cgaacacgat catcgcctcg aacggctcga cctcgagcaa ccgggtgagc
      781 gcgtccatct tccggtgccc ggagacctgg atgtagcgct gcgagatgtt gtcggcggtc
      841 gcggtcttgg actcgacctt tacctggacc gggtcgtgca ggtatttgtt ggtgatcttg
      901 cggatggcgg gcggcatcgt ggcggagaac agcgcgacct gcttgtactc aggcgtgcct
      961 tcaaggatgc gttcgacgtc ctcggcgaag cccatctgga gcatctcgtc ggcctcgtcg
     1021 agcaccaggt agtcgaggtg tgagaggtcg agggtgccct tctcgaggtg gtcgatgacc
     1081 cggcccggag tgccgacgac gacctgggcg ccacgcttga gcccggccaa ctgcggaccg
     1141 taggaggcgc cgccatagat cggcagcacg ttcaccgcca ggcgcgcgcc gtagcggctg
     1201 aacgcctcgg cgacctgaag cgcgagctct cgcgtcggcg caagcaccag cgcctgggtg
     1261 atgcggctcg acgtgtcgat cttcgacaga atcgggatgg cgaacgccgc cgtcttgccc
     1321 gtgccggtct gtgcgaggcc gaccacgtca gaccccgcga tcatcgcggg gatggtcgcc
     1381 gcctggatcg gcgacggcga ctcgtatccg acgtcggcta cggcctgcag caccgcgggg
     1441 tgaatctgca ggtcggcaaa ggtggtttgc gcggcgaacg cgtcgctgtc gggcgtggtc
     1501 atcggggtat cagtctaatc gcccgcgccc gctcgacccg ccgcgccgcc tgccgggtca
     1561 ccggccgagt tggcggtacg gtgcccaact gtgaaaaggc gtcccgggcc gctcggccgg
     1621 cgacatctgg cggcgaccgc tgccgcggcg ggcgtcctcc tgctggccgg ctgcggatcg
     1681 ggtgactcga cggtctccaa gacgccgaat gccagcacaa ccaccgccgc accggcaccc
     1741 gcgaccgcgc agcccgcacc gccacgcccc agcgccgcgc ccagcacggt cgcgcccgac
     1801 ccgtgcgccg tgaacctcgc cgcgcccgag atcgccaagg ccgtctccga gctgccgcgc
     1861 gatccccgca gcgggcaggc gtggagtccc gagccgctgg caggcacgta caacgaatgc
     1921 gcccagctgt cggcgatcat cgtcaaggcc aacaccaacg ccgagaaccc caacacccgc
     1981 gcggtgctgt ttcacctcgg caagttcatc cctacaggcg tgccggacac ctacgggttc
     2041 aacagcgtcc agccgtcgga aagcacgggt gacaccgtcg cgcttgtgta ctccaacggc
     2101 acgcccgggc tggacagcgt ggtgaaattc cgctggaacg gcgcaggcgt cgagctgatc
     2161 ggcaacacgc ccggctgacc ttccgccgaa acgtaccttt gggtgacgac acgccgcgaa
     2221 attccgccca aatgcacgtt tgggcgacag tcagcggggg ctgactgtcg gccggctcac
     2281 ctacagttgg cacgtgttcg ttgcagacga tcgagtgatc tacagcgcct ccgacctcgc
     2341 agccgcggcc cgctgcgagt acgcgctgct gcgctcgttc gacgccaggc tcggatgggg
     2401 tccgccggtc gccgccgagg acgaattgct ggcccgcacg gcgaaactcg gcggcgagca
     2461 cgaacagcgc catctggacg aattgcggtc ggcgacagag ggcacggtgg ccgtcatcgg
     2521 tcggcccgat tacaccgtcg ccggtctcga ggcggccgcc gaagcgacct tgcgggcggt
     2581 gcggcggcgg gcgccggtga tttaccaggc ggccatgttc gacggccggt tcgtcggttt
     2641 cgccgacttc ctggtgttcg acggcgagcg ctaccggttg cgcgatacga agctcgcgcg
     2701 gtcggtcaag gtcgaggcgc tcttgcagct ggcggcgtac gcggagaccc tggccgcggc
     2761 gggcgtgccg gtctccgacg aggtcgagtt ggtgctgggc gacaccgcca ccgtcggcta
     2821 ccgcgtcgat gagctggtgc cggtgtaccg gccgcggcga gccgaactgg aacgcctgct
     2881 cgacggccat cttgccggcg gaagggcggt cagatggtcc gacgagcacg tccgcgcctg
     2941 cttccggtgc ccggagtgca ccgatcaagt ccgcgagcac gacgacctgc tgcttgtcgc
     3001 cgggatgcgg gccagccagc gcgcccggtt catcgacgcg ggcatcgcca ccgagaccga
     3061 ccttgcccgg cacgacggcg ctgttccgga gttgtccgcg cgtacggtcg ccacgctgag
     3121 cgcgcaggcg cgcttgcagc tgacgccgcg catcgacgga aagccgccgt atgagatcgc
     3181 cgacgcgcag ccgttgatgg tgctgcccga gcccgacaag ggcgacttgt tcttcgactt
     3241 cgagggcgat ccgttgtgga cggacgacgg ccgtgaatgg ggcctggagt atctgttcgg
     3301 cgttctcgac accgccgacg atttcaagcc gctgtgggcg cacaaccgca ccgatgagcg
     3361 ccaggcgctg cgcgacttcc tgaagatggt ccggcacagg cggaggcgct ttccgcacat
     3421 gcacatctat cactacgcgg cgtatgagaa gaccgcgctg ctgcgcctgg ccgggcgcta
     3481 cggcgagggt gaagacgaag tggacgacct gcttcgcaac ggcgttctcg tcgacctcta
     3541 tcccttggtg cgaaagagcg ttcgggtcgg cacggagaac tacagcctca agtcgctcga
     3601 gccgctgtac atgggtgggc agcttcgcag cggggatgtc acgacggcta ccgactcgat
     3661 cacgcagtac gcgaagtact gcgagttgca ggccgcggga cgggacgacg acgcggcgat
     3721 cgtcctgaag gagatcgagg actacaaccg atatgactgc acgtcgacgc gcaagctgcg
     3781 ggactggttg atctgtcggg cgatcgagcg cgccgtgcca cctgtcggcg cgcagccggt
     3841 cgccgacagc gcccccatcg ccgacgacga cacgttggcg cgcacgctgg cgaagttcgt
     3901 cggcgacgaa cccgccggcc gcagccccga ccagacggcg gtggcgatga tcgcggcggc
     3961 ccgcggctat caccggcgcg aggacaagcc gtactggtgg ggacatttcg accggctgaa
     4021 caatccggtc gacgagtggg gcgacaactc cgacgtcttc atcgccgaga aggctgtagt
     4081 cgaatccgat tggcacacac caccgaaagc gcgcaagccg cagcggtggg tgcggctcta
     4141 cggcggcatc gcgacgggcg agctgagcaa gtcgatgtac gcgctctacg agccgccctc
     4201 ccctgccggc ctgaccgatg acaccgaacg cagggcgttc gggtcggtca ccgtgacgga
     4261 gtgtgacgat ccggccgcac cgaccgaggt gctcgtcgtc gaacgggaac ccaggaaggg
     4321 cggcccattc gatcaatgcc cgttcgcgct gacccccggg ccgccgatct cgacggctcc
     4381 cctgcgcgac tcgatcgagg cgaccgccac cgagatcgct gcgggtctgc cccagcttcc
     4441 gcccagcgcg gtcatcgaca tcctgctgcg ctgtgcaccg cggaccggca gcggggcgcc
     4501 gctcccgcga aatccggtgg cggccgagtc gatcaccgca gcactgctcg acctcgactc
     4561 gtcgtacgtg gcggtgcacg gaccaccggg aacgggtaag accttcacgg cggcagcggt
     4621 gatcgcgaat ctcgtcaagc acagcggctg gcgcatcggt gtcgtcgcgc agtcgcacgc
     4681 cgtggtcgag aatctgttcc gcgacgtcat cgctgccgga gtcgacccgg cgctggtggc
     4741 caagaagaag cacggcaccg acgcgtgctg gcacgagatc gacgacaagg agtatccggc
     4801 gttcatcgcc gaccatgacg ggtgcgtgat cggcggcacc gcgtgggact tcgccaatcc
     4861 cggccgcgtg cctcgaaaca gcctcgatct gctggtcatc gaggaggccg gccaatacag
     4921 cctggccaac accatcgcgg tggcatcggc cgcgagaaac ctactgctgc ttggggatcc
     4981 gcagcagcta ccgcaagtca gccagggcac ccaccccgaa ccggtcgaca cgtcggcact
     5041 gggctggctg gtcgacggca ggcacacgct gcccgccgaa ctcggctact tcctcgacgt
     5101 gtcctaccgc atgcacccag cggtctgcgc ggcggtgtcg cgcctgtcct acgagggccg
     5161 gctgcgctcg ttcgacggcg tgacggcggc gcgcacgctc gccggccagg cccccggcgt
     5221 tcgggtgctc accgtcgacc atgatggcaa ctcgaccgac agccctgaag aggccgacgc
     5281 catcgtggcc gaggtgaaac ggctgctcgg atcggcgtgg accgacgagc acggaacccg
     5341 ccctctgaaa caggacgacg tgctggtggt gacgccctac aacgcgcagg tggtgctgct
     5401 gcgccaacgt ttcgacgcgg ccggactggc cgatgtgcgg gcgggcaccg tcgacaagtt
     5461 ccagggcagg caggcgcccg tggtgttcgt ctcgatgacg gcgtcctcca ttgacgacgt
     5521 gccccgggga atcgcgttcc tactgaaccg caatcggctc aacgtcgcga tcagccgggc
     5581 caagtacgcg agcatcatcg tccgatccga gacgctgacc gaataccttc cgacgacgcc
     5641 ggaccgcctg atcgaattgg gcgcgttcct gtcgctgccg tcgtgtgata aacccacgga
     5701 gtgaccgaaa cgctggccga ccggctcgcc gggatcgtcg gcgccgcgca cgtcagcg
//
LOCUS       DASYLX010000580         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3209177,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000580 DASYLX010000000
VERSION     DASYLX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3209177"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGP27_13935"
     CDS             complement(<1..621)
                     /locus_tag="VGP27_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEV7852433.1"
                     /translation="MSGATSFTSVPTVDISGLRSSERVQRERVAAEIGRAASEVGFFY
                     VSGTGVDDELFVRMLAVTKEYFALPVEEKMRSYIGLSRCHRGYVPVGEEGVETGTADL
                     KEAFDTALDLPPDDPDYVAGNPMLGPNTWPDLPGFAEAVTAYYDAVLAVGHRLLWAFA
                     VALGEDPDVFSRHATKTPSQLRLVHYPYNPDAEDGHGIGAHTDYECF"
     gene            complement(618..1151)
                     /locus_tag="VGP27_13940"
     CDS             complement(618..1151)
                     /locus_tag="VGP27_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852434.1"
                     /translation="MSTPVVADCAGLWRRTLLIGADGSRDTGTDVQWLQGITAYVDSR
                     GFAGALGQNGDIFEWRRDIDLGPPNQLPDEGSMRWDGRALVETGVHEDYVEHWVRDTM
                     PTEPGGALFLTAPDGGNGLLLRVGDLFGWAGAGAVAIDTITSGQWDALRIELFAEHVE
                     ANGVRWRIEQREGNVNP"
     gene            complement(1148..2161)
                     /gene="alc"
                     /locus_tag="VGP27_13945"
     CDS             complement(1148..2161)
                     /gene="alc"
                     /locus_tag="VGP27_13945"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEV7852435.1"
                     /translation="MTEVDLASRSLGGSVVAASDESFGFKERLIDPAEPAFVPGTYDL
                     RGEVVDGWETRRHAGADGDWVILRLGAPGRLHAVDIDTRFFAGNHPTACRVDGCALGR
                     TADPTAPDVRWTPLVEHTVLKEDSRNLLSVRDQRRWTHVRLRLDSDGGVARLRAYGKV
                     IPDPALWTDVTVEVSGLEQGGRVEWCSDSFYSHACALIEPTRPRNMGDGWETRRRRDV
                     GPESHDAVLIGFAVPADLVRLEIDTSYFVFNASDEVSVLGSRARPDGEHGWALVPFDI
                     PVLPRSRLVADARQLFTVDARGVTALRVQAYPDGGIARVRALGMPTDEGLEWLRRQWE
                     ESK"
     gene            complement(2259..>2796)
                     /locus_tag="VGP27_13950"
     CDS             complement(2259..>2796)
                     /locus_tag="VGP27_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV7852436.1"
                     /translation="LAGFGEHGKDALQEMLTVPVFDIAECAAHVAHLIGRRFSVVTTL
                     SRSIAPIEDRLLLAGLNAHCTSVRACGLGTAEVDADPAGAVAAIVDEAARAVSEDGAD
                     VICLGCAGMAGVAEAISRKVGLPAVDGVAAAIALAQAVVGLALSTSKAGVYAPGPDNP
                     RSHWPISRALGLAERGEE"
BASE COUNT          424 a         1001 c          897 g          474 t
ORIGIN      
        1 gaaacactcg tagtcagtgt gtgcgccgat gccgtgtccg tcctctgcgt cggggttgta
       61 cgggtaatgc accaggcgaa gttggctcgg ggtcttggtg gcgtgccgcg agaatacgtc
      121 ggggtcttct ccgagagcca ctgcgaacgc ccacagcagc cgatgtccga cggcgaggac
      181 tgcgtcgtag taggcggtaa ccgcctcggc gaagcccggc aggtccggcc acgtgttggg
      241 gcccagcatc gggttgcccg ctacgtaatc ggggtcgtcg ggcggtaggt ccagcgcggt
      301 gtcgaacgcc tccttcagat cggccgtgcc ggtctccact ccctcctcgc cgactggcac
      361 atagccgcgg tggcaccgcg acaagccgat gtagctacgc attttttcct cgaccggtaa
      421 agcgaagtac tctttggtaa cggcgagcat gcggacgaac agctcatcgt cgacacccgt
      481 accgctgacg tagaagaagc cgacttcgct tgctgccctg ccgatctcgg ccgccacccg
      541 ctcgcgctgc acacgttccg atgatcgcag cccgctgatg tcgaccgtcg gcacggaggt
      601 gaatgacgtt gcgccgctca tggattcacg ttcccttcgc gttgttcgat tctccagcgc
      661 acgccgttcg cctcgacgtg ttcggcaaac agctcgattc tcagggcgtc ccactgcccg
      721 gatgtgatgg tgtcgatcgc gacggcaccc gcgccggccc agccgaacag gtcgccgaca
      781 cgcaacagga ggccgttgcc accgtcgggt gcggtgagaa agagtgcgcc gccgggttct
      841 gtcggcatcg tgtcgcggac ccagtgctcg acgtagtcct cgtgtacacc cgtctcgacc
      901 agtgcccgac cgtcccaccg catcgagccc tcgtccggca actggttcgg cggtccgagg
      961 tcgatatcgc ggcgccactc gaagatgtca ccgttctgac ccagcgcgcc cgcgaagcct
     1021 cgcgagtcga cgtaggcggt gatgccttgc aaccactgca cgtctgtgcc ggtgtcgcgg
     1081 gagccgtcgg cgccgatcag caacgtgcgt cgccacaggc ctgcacagtc ggccaccacc
     1141 ggggtgctca cttcgactct tcccactgcc ggcgcagcca ttccaggccc tcatcggttg
     1201 gcatgccgag cgcgcgcacc cgtgcgattc ccccgtccgg gtatgcctgc acccgcagtg
     1261 ccgtgacgcc gcgggcgtcc acggtgaaca gttgtcgggc gtcggcgacc agccggctac
     1321 gtggcagcac cggtatgtcg aacggcacca gcgcccagcc gtgttcaccg tcagggcgtg
     1381 cgcgactgcc gagaacggaa acctcatccg atgcgttgaa cacgaagtac gaggtgtcga
     1441 tctccaggcg caccagatcg gcgggcacag cgaagccgat cagcacggcg tcgtggctct
     1501 cgggtccgac gtcgcggcgc ctgcgggtct cccagccgtc gcccatgttc cgtggccttg
     1561 tcggttcgat gagcgcgcag gcgtgcgaat agaagctgtc gctgcaccat tcgacgcgcc
     1621 caccctgctc cagcccggac acctcgacgg tgacatctgt ccagagcgca gggtcgggga
     1681 tcaccttgcc gtaggcgcgt agtcgcgcca ccccgccgtc ggagtccagc cggagccgca
     1741 cgtgggtcca gcgccgctga tctcgcactg acaacaggtt ccgcgaatcc tccttcagca
     1801 cggtgtgttc caccaacggt gtccacctga catccggtgc ggtggggtcc gccgtcctgc
     1861 ccagagcgca tccgtccacc cggcacgcgg ttgggtggtt tccggcgaag aagcgggtgt
     1921 cgatgtcgac ggcgtgcaac cgtcccgggg caccaaggcg caggatcacc cagtcgccgt
     1981 cggcgcctgc atgcctgcgc gtctcccacc cgtcgacgac ttcgcctcgc aggtcgtaag
     2041 tgcccggcac gaaggccggt tcggccgggt cgatgagccg ctccttgaac ccgaaggatt
     2101 cgtcactcgc ggcgacgacg ctgccgccca gcgagcggga tgccaaatcc acctccgtca
     2161 ttcctcgcct ccaggtcgtc ccctgccctg cgggcgtggg cccaccggtc ctcgctcgtc
     2221 cttcgctgcg atcctcactc gccgtcgact tcgggccgtc attcctcgcc tctctcagcc
     2281 aggcccagcg cccgcgagat cggccagtgg ctgcgcgggt tgtccggtcc cggtgcgtag
     2341 acgcctgcct tgctggtgga cagcgcgagg ccgacgacgg cctgcgccaa cgcgatcgcc
     2401 gcggcgacgc cgtcaacggc gggcagcccg actttgcgtg agatcgcctc cgccacgcct
     2461 gccatccctg cgcagccgag gcagatcacg tcggcaccgt cctcgctgac agcgcgggcc
     2521 gcctcgtcca cgatggcggc gaccgcaccg gccggatcgg cgtcaacctc ggcggtgccg
     2581 aggccgcacg cgcgcaccga tgtgcaatgt gcgttcagcc cggcgagcag cagccggtcc
     2641 tcgatcggcg ctatcgaacg gctcagcgtc gtgaccaccg agaaccgtcg cccgatgagg
     2701 tgggcgacgt gcgcggcgca ctccgcgatg tcgaaaaccg gtaccgtcag catctcctgc
     2761 agcgcgtcct tgccgtgctc gccgaatccg gcaagc
//
LOCUS       DASYLX010000581         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1344518,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000581 DASYLX010000000
VERSION     DASYLX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344518"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGP27_13955"
     CDS             complement(<1..516)
                     /locus_tag="VGP27_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852437.1"
                     /translation="MSKSPLRRLADQLVLASMRPPIAPALMLHRPGQRDIDLQGKRIL
                     LTGASSGIGEAAAEKFARRGATVVVVARRKDLLNEVADRILADGGDALAIDCDLADLD
                     AVDALAARVEKEIGGVDILVNNAGKSIRRPLAESLERWHDVERTMALNYYSPLRLIRA
                     LAPGMLERGDGH"
     gene            572..1078
                     /locus_tag="VGP27_13960"
     CDS             572..1078
                     /locus_tag="VGP27_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7852438.1"
                     /translation="MAINGKLDTRFSEAAEAVGWQQVSDALTAAELYWLTTVRKDGRP
                     HITPLVGAWVDDAFVFCTGPEEQKAQNLNGSTSVAVTTGVNTWDDGLDIVVEGDAERV
                     TGVDTLTKLADVVREKYHGDWDFTPTDGFGHSDGDGDRHIAHVYRVPPTKVLAFAKSP
                     HGQTTFRF"
     gene            1293..>2056
                     /locus_tag="VGP27_13965"
     CDS             1293..>2056
                     /locus_tag="VGP27_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEV7852439.1"
                     /translation="MNSIVSPLVWIITVVVLLGIIILDLAVIGRRQQTVTTKDAVRWV
                     LVYIGLAVAFAVGLFIFAPGASGQEFVAGYITEYSLSVDNLFVFMIIMARFGVPELAQ
                     DKVLYIGIILSLVFRAVFIFAGAAAIAAWSWVFYILGGFLLYTAIQLALEDPDDEPDF
                     HKNPLLRFLRRILPLSEDYDGRKLATRSEKHRMFTPLVIVIAAIGIANVVFALDSIPA
                     IFGLTNDAYIIFTANALALMGLRQLYFLIGGLLEKV"
BASE COUNT          344 a          679 c          661 g          372 t
ORIGIN      
        1 gtggccgtcg ccgcgctcga gcatgccggg cgccagggca cggatcagcc gcagcggcga
       61 gtagtagttg agggccatcg tccgctcgac gtcatgccag cgctccagcg actcggccaa
      121 cgggcggcgg atggacttgc ctgcattgtt gacgagaatg tccacgccgc cgatctcctt
      181 ctcgacgcga gcggcgagcg catcgaccgc gtcgaggtcg gccagatcgc agtcgatggc
      241 gagcgcgtcg ccgccgtcgg cgagtatccg gtcggcgact tcgttgagca gatccttgcg
      301 tcgggcgacc acgacgacgg tggcgccgcg gcgggcgaac ttctcggcgg ccgcctcgcc
      361 gatgcccgat gaggcgccgg tgagcaggat gcgcttgcct tgcaggtcga tgtcccgctg
      421 acccggccgg tgcaacatca gcgcaggagc gatcggcggt cgcatgctgg ccagcaccag
      481 ctggtcggcc agccgccgca gtgggctctt actcacccgt ggagtgtatg gaccgcgatg
      541 agatggtggc cgtcacaggg tcggtatggg tatggcgata aacggaaagc tcgatacccg
      601 gttcagtgaa gccgccgaag cagtcggctg gcagcaggtc agtgacgcgt tgaccgccgc
      661 cgaactctac tggctgacga ccgtccgcaa ggacgggcgg ccgcacatca cgccgctggt
      721 cggcgcgtgg gtggacgatg cattcgtgtt ctgcaccgga cccgaggagc agaaggcgca
      781 aaacctcaac ggctcgacgt cggtggcggt cacgacgggc gtgaacacgt gggacgacgg
      841 gctcgacatc gtcgtcgagg gcgatgccga acgggtgacc ggtgtggaca cgttgacgaa
      901 gctggccgac gtcgttcgcg agaagtacca cggcgactgg gatttcacgc ctaccgacgg
      961 cttcggtcac agcgacggcg acggcgatcg ccacatcgcg cacgtctacc gcgtgccacc
     1021 caccaaggtg ctggccttcg ccaagtcacc gcacggtcag accacgttcc gcttctaacg
     1081 cccgtcctcc aggtgaaacc cttccccccg catgcgacag attctgcacc gtggactata
     1141 gttctgcttg ttcgtgaagg ggagtattcc tccgcggcac tcgtcgtcat cacggaaggc
     1201 ccaacggccg acccggcggt gccggtctca ggctagggcc tgaggcggaa gagaccttcg
     1261 gaccgtcgct tgctgccgtc cggaggtcga tcgtgaattc tattgtctca ccgctggttt
     1321 ggatcatcac cgtcgtggtg ttgctcggga tcatcatcct cgacctcgcg gtgatcggcc
     1381 gcaggcaaca aaccgtcacc accaaggacg ctgtccgctg ggtcctcgtc tacatcgggc
     1441 tggcggtcgc gttcgccgtc ggactcttca tcttcgcgcc gggcgccagc ggccaggagt
     1501 tcgtcgccgg ctacatcacc gaatacagcc tgagcgtgga caacctcttc gtgttcatga
     1561 tcatcatggc ccggttcggc gtccctgaac tggcacagga caaggtgctc tacatcggga
     1621 tcatcctgtc gctggtcttc cgcgccgtgt tcatcttcgc cggggccgcg gcgatcgccg
     1681 cgtggagctg ggtgttctac atcctcggcg ggttcctgct ctacaccgcg atccagctcg
     1741 cgctcgagga ccccgacgac gaacccgatt tccacaagaa cccgctactg agattcctgc
     1801 gccgcatcct gccgctcagc gaggactacg acggccgcaa gctggccacc cggtcagaga
     1861 aacaccggat gttcaccccg ctggtgatcg tcatcgctgc gatcggcatc gccaacgtcg
     1921 tgttcgccct cgactcgatt ccggcgatct tcggactcac caacgacgcg tacatcatct
     1981 tcaccgccaa cgcgctggcc ctgatgggct tgcggcagct gtacttcctg atcggcgggc
     2041 tgctcgagaa ggtcat
//
LOCUS       DASYLX010000582         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2660789,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000582 DASYLX010000000
VERSION     DASYLX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660789"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VGP27_13970"
     CDS             complement(<1..1241)
                     /locus_tag="VGP27_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEV7852440.1"
                     /translation="MTTFTQQELVDGLVVDDDAQESVRRKRPSRWSLRNWPVRWKVFA
                     IVLVPLILAGAFGGLRIYSNAIQARDLQHAADRAEMVPAIVSYMNALNGAMLAVPAGG
                     DTQGAMSTFDSSRAELQRRLNDTPDVPPDVQKGVTSMLLGGQALMDKVSSNAIGLVDR
                     VTLYAPILLTSEDAITGSVRVDDERIRNQAQGLTRAVGARGQMMMEQLLVNQGSELPE
                     PEQRARMITLAGTEPSTLFGMSQVLGIGSDEAKQLQGEYVRRTALISDPTVPLANNPD
                     MTASLRATDQIATRMIDRTVPAVTGAVKADASAARTEAIRDSAIVVGAILLALLLVIL
                     VARSLIRPLRTLRDSALRVAHEDLAREIERVRAGKEPGPVEPIPVNTTEEIGQVAHAV
                     DELHEQAVFMAGEQSRLQLQVG"
     gene            1486..1950
                     /locus_tag="VGP27_13975"
     CDS             1486..1950
                     /locus_tag="VGP27_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV7852441.1"
                     /translation="MFRVMFFSPRIAPNTGNAIRMVAATGCELHLVEPLGFELSEPKL
                     RRAGLDYHDLASVTVHTDLAAAWEAMAPTRVHAFSAHATTPFADIAYEPGDVLMFGPE
                     PTGLDDATLADPHITSRVRIPMLAGRRSLNLSNAAAVAVYEAWRQNGFSGGE"
     gene            complement(1951..2592)
                     /locus_tag="VGP27_13980"
     CDS             complement(1951..2592)
                     /locus_tag="VGP27_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7852442.1"
                     /translation="MTLNLSVDDLLTTTRSVRKRLDLEKPVPREVLMECLDLALQAPT
                     GSNAQGWQWVFVDDPEKKKALADIYRHNATPYLDTPKPEFGDVRDEQRRLVTSSAKYL
                     NEHLHEVPVLLIPVLAGRPDGAPAGQSAGFWGSLLPAVWSFMLALRSRGLGSAWTTLH
                     LVGKGEKLAADVLGIPFEEYSQGGLFPIAYTKGTDFKRAKRLPAEQFAHWNSW"
BASE COUNT          445 a          940 c          969 g          502 t
ORIGIN      
        1 ccgacctgca gctgcagtcg gctctgctcg cccgccatga agacggcctg ctcgtggagt
       61 tcgtcgacgg catgggcgac ctggccgatc tcctcggtgg tgttcaccgg gatgggttcg
      121 acgggtccgg gttccttacc cgcgcgcact cgttcgatct cacgcgccag gtcttcgtgc
      181 gcgacccgca gggcgctgtc gcgcagtgtg cgcagcggcc ggatcagcga gcgcgccacc
      241 aggatgacga gcagcagggc cagcaggatg gcaccgacca cgatcgccga gtcgcggatc
      301 gcttcggtgc gtgcggccga cgcgtccgcc ttgaccgcac cggtgacggc gggcacggtg
      361 cgatcgatca tccgggtggc gatctggtcg gtggcccgca gcgacgcggt catatccggg
      421 ttgttggcca gcgggaccgt cgggtcggag atgagcgcgg tgcggcggac gtactcgccc
      481 tgaagttgct tggcctcatc cgagccgatg ccgagcacct ggctcatgcc gaacagggtc
      541 gacggctcgg tgcccgcgag cgtgatcatg cgcgcgcgct gctccggctc gggcagctcg
      601 ctgccctggt tgaccagcag ctgctccatc atcatctgac cgcgagcgcc gaccgcgcgg
      661 gtgaggccct gcgcctggtt gcggatgcgt tcgtcgtcaa cgcggaccga gccggtgatc
      721 gcgtcctcgg acgtgagcag aatcggtgcg tagagggtga cccggtcgac cagtccgatg
      781 gcgttggagg acaccttgtc catcaacgcc tggccgccca agagcatgct ggtgacgccc
      841 ttctggacgt ccggcggcac gtcgggtgtg tcgttgagcc gacgctgaag ctccgcgcgg
      901 ctcgagtcga acgtgctcat cgccccctgg gtgtcgccac cggcgggcac ggccagcatc
      961 gcgccgttca gcgcattcat gtagctaacg atcgcgggca ccatttcggc gcgatcggcc
     1021 gcgtgttgca gatcacgggc ttggatcgcg ttcgagtaga tccgcaaccc gccgaaggcg
     1081 cccgccagaa tcagcggcac caaaacgatc gcaaacacct tccagcggac cggccaattc
     1141 ctcaacgacc atcgggaggg acgtttcctg cgcaccgact cctgggcatc gtcgtcgacg
     1201 accagtccat ccaccagctc ctgctgggtg aacgtcgtca ttgctgtccc atcacgccac
     1261 cggctgcttg tccggctttt ccaatttgag cgaggttcat acgcttcctg ctgatttgcc
     1321 cgaatgcggg caggcgttct tcgcctggca attgatcgag tatgccagta gcacgcggcc
     1381 gtttccacaa ttcttactga acagccaagg ttcgcgcact ctgccagaac cctcttagtc
     1441 ggtgtccgag caaatgccga acggggcgcc tgcaatcatc gtcggatgtt ccgggtgatg
     1501 tttttctccc cccgcatcgc gcccaacacg ggtaacgcga ttcggatggt cgcggccaca
     1561 ggttgcgagc tgcatctcgt cgagccgctc ggcttcgagc tatccgaacc gaagctgcgc
     1621 cgagccgggc tggactacca cgacctcgcg tcggtgacgg tgcacacgga tctggctgcc
     1681 gcatgggagg ccatggcacc gacgcgggtg cacgccttca gcgcgcacgc gacgacgccg
     1741 tttgcggaca tcgcctatga acctggcgac gtgttgatgt tcggtcccga gccgacgggt
     1801 ctggacgacg cgacactcgc cgatccccac atcacctcgc gggtgcgcat cccgatgctc
     1861 gcgggcaggc gctcgctgaa tctgtcgaac gccgccgccg tcgccgtcta cgaggcgtgg
     1921 cggcagaacg gcttcagcgg cggcgagtag tcaccacgag ttccagtgcg cgaactgctc
     1981 ggcgggcagg cgctttgctc gcttgaaatc ggtgcccttg gtgtaggcga tcgggaagag
     2041 cccgccctgg ctgtactcct cgaacggaat gccgaggacg tcggcggcca gcttctcgcc
     2101 cttgccgacg agatgcagcg tcgtccatgc cgagccgagg ccgcgcgagc gcagcgccag
     2161 catgaagctc cacacggcgg gcagcagcga gccccagaat ccggcgctct gcccggcggg
     2221 tgcgccgtcg gggcggcctg ccagaaccgg gatcaacaag accggcacct catgcaggtg
     2281 ctcgttgaga tacttcgccg agctggtgac gagccggcgc tgctcgtcgc gcacgtcgcc
     2341 gaactccggc ttgggcgtgt cgaggtaggg cgtcgcgttg tgtcggtaga tgtcggcgag
     2401 cgccttcttc ttctccgggt cgtcgacgaa aacccactgc cagccctggg cgttggaacc
     2461 cgtcggcgcc tgcagcgcca ggtcgagaca ctccatcagc acctcgcggg gcaccggctt
     2521 ctccaggtcg agccgcttgc ggaccgagcg ggtggtcgtc aggaggtcgt cgacggacag
     2581 gttgagtgtc atgttgcgag gttactgagt tcccgcgagc gcgcgcgtcc gcacaccgat
     2641 tgacaatgcg ccgtgcggat gccgaggtct gcgcacgctc gggcagcgcg acggtgcgca
     2701 gaataccggt gaaaagcgga gtgtctgcgt gcagacccgc actgtcgcgg agatgtgggt
     2761 cagcggaaat cgcggctgcg ggaggcgatc cgcatgtcga gcttgctgag ccggtcggcg
     2821 acgacggtga ccgcgccggt ggcgttctgg acctgg
//
LOCUS       DASYLX010000583         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1234496,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000583 DASYLX010000000
VERSION     DASYLX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234496"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1843)
                     /locus_tag="VGP27_13985"
                     /pseudo
     CDS             complement(1..1843)
                     /locus_tag="VGP27_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884608.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          256 a          603 c          665 g          319 t
ORIGIN      
        1 tcggcgtgcg ccatgaccgc ctggtggtgc tcgatgacga tgaccgactt gccggagtcg
       61 acgagccggt cgagcaggcc gagcagctgc tcgacgtcgg cgaggtggag gccggtggtc
      121 ggctcgtcga ggacgtcgac gtcgcccttc tcggccatgt gggtggcaag ctggagccgc
      181 tgccgctcgc cgccggacag cgtagtgagc ggctggccga ggctgaggta gcggagccgg
      241 acgtcggcga gccggtcgag gatggcgtgc gcggtcggcg tgcgcgcctc gccggcgcca
      301 aagaactcct cggcctcggt caccgacatc gcgggcacct cgctgacgtc gcggccgccg
      361 aggtggtggt ccagcaccga tgcctggaat cgcttcctct ggcattcctc gcaggtggtg
      421 gcgacgccgg tcatcatcac caggtcggtg tagatgacgc cagcgccgtt gctgttgggg
      481 caggcgtcct cggagttggc gctgaacagc gccggcttca cgccgttggc cttcgcgaac
      541 gccttgcgga tcgcgccctg gtcgatcgac accacatccg cgccggctgg cccagacaga
      601 tctggcagcg acccgtgcac gagcgaactc ttgccggagc cggcgacgcc ggtgacgacg
      661 accagcaccc cgagcgggat gtcgacgtcg acgtcgcgca ggttgtgcgc cgtcgcgccg
      721 cggatctcca gggtgccggt gggcgtccgc accgtctcca cgagggcggc ccggtcgtcg
      781 agatggcggc ctgtgatggt gccgctggcc cgcagcccct cgacggtgcc ctcgaagcag
      841 acggtgccgc ccgcgtaccg gcgccggggc cgaggtcgat gacgtggtcg gcgatcgcga
      901 tggtctccgg cttgtgctcc acgacgagca ccgtgttgcc cttgtcccac agctgcagca
      961 gcaggtcgtt ctcgatgccc ctgatccgcg aggcgcgggc ggcctcgttg agccggctgc
     1021 cgccacactc cggacaggcg gtgaaggtga ccgcccggtc cacgaacgcc cggatgtgcg
     1081 gctgcatcgc ctccttgtcc ttggagagga acgacttctg gaccttcgga tcagcccctc
     1141 gtaggtgagg ttgacgccgt ttaccttcac cttggtcggc tcgcggtgga ggaagtcgtg
     1201 cagctccttc ttggtgtacc tgcggatcag cttgtccggg tcgagcaagc ccgattcggt
     1261 gaagatcctt actgtccacc agccgtccac cttgtagccg gggatggtga tcgcgccctc
     1321 ggcgagcgac ttggagtcat cgaagagctg ggtgaggtcg atgtcggaga ccgtgccccg
     1381 gccttcgcag cgcggacaca tgccgccggt acgggtgaag gtctgtctca cggtcttgcc
     1441 ggcaccgcgt tcgatcgtga tcgcaccgct cgcccggacc gaggggacgt tgaaggcgaa
     1501 cgcggtgggc gagccgatgt gcggctgccc gagtcggctg aacaggatgc gcagcatcgc
     1561 gttggcgttg gcttcggtgg cggtgccgac ggtggagcgg gggtcagcac ccatccgctg
     1621 ctggtcgacg atgatcgcgg tcgtcagccc gtcgagtacg tcgacctcgg gccgcgccag
     1681 cgtcggcatg aagccctgta cgaggcgctg taggtctcgt tgatcatccg ctgcgactcc
     1741 gcggcgatcg tgccgaacac cagcgagctc ttgcccgagc cggagacgcc ggtgaacacc
     1801 gtcagccggc gcttcgggat ctcgacgctg acgtccttga ggt
//
LOCUS       DASYLX010000584         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_577754,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000584 DASYLX010000000
VERSION     DASYLX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577754"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGP27_13990"
     CDS             <1..184
                     /locus_tag="VGP27_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEV7852443.1"
                     /translation="ASPYDLRDYGFEPIAVETPAGRAAYARAQQDIAGRAAPLRTRIA
                     DRCDELLAAAVAAVGN"
     gene            257..2464
                     /locus_tag="VGP27_13995"
     CDS             257..2464
                     /locus_tag="VGP27_13995"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEV7852444.1"
                     /translation="MTDRVTVGNLRIAQTLYDFINNEALPGTDIDPDTFWSGVDKVVA
                     DLTPKNQELLAHRDELQAQIDKWHRHRVIEPLDPEAYKQFLTEIGYLQPEPDDFTITT
                     SGVDDEITTMAGPQLVVPVLNARFALNAANARWGSLYDALYGTDVIPEDDGAEPAAGS
                     GGEYNPVRGDKVIAYARNFLDEAVPLESGSWADATGLSLDDGVLQVGTADGSVGLASP
                     EKFVGYTGELGSPDWSVLLVNNGLHIEILIDPESPVGSTDKAGIKDVVLESAITTIQD
                     FEDSVAAVDAEDKVLGYRNWLGLNRGDLAEDVTKGDKTFTRVLNEDRTFQTPDGGELT
                     LPGRSLLFVRNVGHLMTNDAIVDADGNEVPEGIQDALFTSLIASHGLKAGDGNGPLVN
                     SRTGSIYIVKPKMHGPDEVAFTSELFARVEDVLGLPPATIKVGIMDEERRTTLNLKAC
                     IKAAADRVVFINTGFLDRTGDEIHTSMEAGPMIRKGAMKSTAWIKAYEDANVDTGLAA
                     GLSGRAQIGKGMWAMTELMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHYHQVDVF
                     EVQKELEGKTRTTIDELLTIPLAKELAWAPEEIREEVDNNCQSILGYVVRWIDAGVGC
                     SKVPDIHDVALMEDRATLRISSQLLANWLRHGVITDDDVKASLRRMAAVVDKQNEADP
                     EFRPMAPDPDASIAFQAAQELILSGAQQPNGYTEPILHRRRREFKAANPSNAG"
BASE COUNT          529 a          867 c          874 g          378 t
ORIGIN      
        1 ggcgagcccc tatgatctcc gcgactacgg tttcgagccc atcgccgtcg aaacgcctgc
       61 cggtcgggcc gcctacgccc gcgcacagca ggacatcgcc ggcagggcgg cgccgctgcg
      121 gaccaggatc gccgatcgct gcgacgaatt gctggcggcg gccgtagccg cggttggcaa
      181 ttagccctgc tacccgcggg taaactggac cgacggcagt gcgccacgcg gcgctgtgca
      241 gccgagggag gaaaatatga ctgatcgcgt gacggtggga aacctgcgta tcgcccagac
      301 gttgtacgac ttcatcaaca acgaggcgct accaggcacc gacatcgatc ccgacacctt
      361 ctggtcggga gtcgacaagg tcgtcgccga tctgaccccg aagaaccagg aactgctggc
      421 tcaccgggac gaactgcagg cacaaataga caagtggcac cgtcaccggg tcatcgagcc
      481 tctcgaccca gaggcctaca agcagttcct caccgagatc ggctatcttc agcccgagcc
      541 tgacgacttc accatcacca cgtccggcgt cgacgacgag atcaccacca tggccggccc
      601 gcagctggtg gtgcccgtcc tcaacgcgcg gttcgcgctc aacgccgcca atgcgcggtg
      661 gggctcgctg tacgacgcgc tctacggcac cgacgtcatc cccgaggacg acggggctga
      721 gccggcggcc ggatccggcg gcgaatataa cccagtccgc ggggacaagg tcatcgccta
      781 cgccaggaac ttcctggacg aggcggtgcc actggagtcg gggtcctggg cagatgcgac
      841 aggactgagc ctcgacgacg gggtgctgca ggtgggtacc gccgacggat cggtcggcct
      901 ggcgagtccg gagaagttcg tcggctacac cggcgaactc ggttcccccg actggtcggt
      961 gctgctggtc aacaacggac tgcacatcga gatcctgatc gacccggagt cacccgtcgg
     1021 ctcgaccgac aaggccggca tcaaggacgt cgtgctggag tccgcgatca ccacgatcca
     1081 ggacttcgag gactcggtgg ccgccgtcga cgccgaggac aaggtgctcg gctaccgcaa
     1141 ctggctcggg ctgaacaggg gcgatctcgc cgaggacgtc acaaagggcg acaagacgtt
     1201 cacccgcgta ctgaacgagg accggacttt tcagacaccc gacggcggcg agctcacgct
     1261 gcccgggcgc agcctgctgt tcgtgcgcaa cgtgggacac ctgatgacca acgacgcgat
     1321 cgtcgacgcc gacggcaacg aagtgcccga gggcatccag gatgcgttgt tcaccagcct
     1381 gatcgcgagt cacggcctca aggcgggcga tggcaacggg ccgttggtca acagtcgcac
     1441 cgggtcgatc tacatcgtga agccgaagat gcacggaccc gacgaggtgg cgttcacctc
     1501 cgagctgttc gccagggttg aggacgtgct cgggctgccg ccggccacca tcaaggtcgg
     1561 catcatggat gaggagcgcc gcaccaccct caacctgaag gcgtgcatca aggcagccgc
     1621 cgatcgggtc gtcttcatca acaccggatt cctcgaccgc accggcgacg agattcacac
     1681 ctcgatggag gcgggcccga tgatccgcaa gggcgcgatg aagagcaccg cgtggatcaa
     1741 ggcctacgag gacgccaacg tcgacacggg tctggcagcc ggcctctccg gcagggccca
     1801 gatcggtaag ggcatgtggg cgatgaccga gctgatggcc gacatggtcg agcagaagat
     1861 cggtcagccg aaggcaggcg ccaccacggc atgggtgccg tcgccgaccg ctgccacgct
     1921 gcacgccatg cactaccacc aggtggacgt gttcgaggtg cagaaggaac tcgagggaaa
     1981 gacccgcacc accatcgacg agctgctcac cattccgttg gccaaggagc tggcctgggc
     2041 gccggaggag atccgcgaag aggtcgacaa caactgccaa tcgattctgg gctacgtggt
     2101 gcgctggatc gacgcaggcg tcggctgctc caaggtgccc gacattcacg acgtcgcgct
     2161 gatggaggac cgtgccacgc tgcgtatctc cagccagttg ctcgccaact ggctgcggca
     2221 cggcgtcatc accgacgatg acgtaaaggc cagcctgcgc aggatggccg ccgtggtgga
     2281 caagcagaac gaggccgatc cggagttccg gccgatggcg cccgaccccg acgccagcat
     2341 cgccttccag gccgcgcagg agctgattct gtcgggggcg cagcagccca acggctacac
     2401 cgagccgatc ctgcaccggc gtcggcgcga gttcaaagcg gcgaatccca gcaacgccgg
     2461 ctgaccggca agtttcggta tgtggataga cttcggcgag gcagctgagc tgccagcaat
     2521 gatcagcgaa cggagaggac gcaggcgcag gcgtgggtag gcacagcatt cccgaccccg
     2581 aggagtcctc aggcggtccc cccgagggcg cagccgatgc gaccgaatcg ttcgaacagc
     2641 agcaggtc
//
LOCUS       DASYLX010000585         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2881800,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000585 DASYLX010000000
VERSION     DASYLX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2881800"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..364
                     /locus_tag="VGP27_14000"
     CDS             80..364
                     /locus_tag="VGP27_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852445.1"
                     /translation="MPVLHIEHEISELAVWLDAFNKMAPAREAAGVTRTEVYRPNDDP
                     NYVVINLSFDNLDAATTFREFLINQIWASPNSAPALVGPPTARVLEEVQL"
     gene            complement(445..>1660)
                     /gene="tgt"
                     /locus_tag="VGP27_14005"
     CDS             complement(445..>1660)
                     /gene="tgt"
                     /locus_tag="VGP27_14005"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV7852446.1"
                     /translation="EPSFSVEAEIPGRLGRAGVIRTPHGDIHTPAFIAVGTQATVKAV
                     LPEAMKELGAQALLANAYHLYLQPGADIIDEAGGLGTFMNWPGPTFTDSGGFQVLSLG
                     AGFRKVLAMDTERVQADDVIAEGKERLARVDDDGVTFTSHLDGSTHRFTPEVSIRIQH
                     QIGADIIFAFDELTTLVNTRGYQEESVQRTHRWAVRCLAEHRRMTAAHPDRHPQALFG
                     VVQGAQYEDLRREATRGLVSIVDDEGRGFDGYGIGGALEKQNLATIVGWVSSELPADK
                     PRHLLGISEPDDLFHAVEAGADTFDCVSPSRVARNAAVYSATGRYNITGARYRRDFTP
                     IDADCDCYTCAHYTRAYIHHLFKAKEILAATLCTIHNERFIIRLVDRIRTAIKAGEFD
                     ELRSDVLGRYYQ"
BASE COUNT          269 a          512 c          558 g          321 t
ORIGIN      
        1 catccggtcg agggtgctcg cgactcgcgc atcaagcaag gtggaagtca tacccacgaa
       61 ggtagtccac actgttgaca tgcccgtttt gcatatcgag catgagatca gcgaactggc
      121 ggtctggctc gatgcgttca acaagatggc acccgcccgg gaagcggcgg gtgtgacacg
      181 caccgaggtg taccggccga acgacgaccc gaattacgtc gtgatcaatc tcagtttcga
      241 caatctcgat gcggcaacga cattccgtga attcctgatc aaccagatct gggcatcgcc
      301 gaattcggca ccggctctgg tgggccctcc gactgcgcgg gtcttggagg aagtgcagct
      361 ctagacgggt gccccgccgg gcgccgagat cgacgttttg gcgggcttta ctcgcacttt
      421 ttcgcccatt tgtgagtttg ggcgtcactg gtagtagcgg cccagcacat cgctgcgcag
      481 ttcgtcgaat tcacccgctt tgatggccgt ccggatccgg tcgacgagcc tgatgatgaa
      541 ccgctcgttg tggatcgtgc acagggtcgc ggccaggatt tccttggcct tgaacaggtg
      601 atggatgtag gcgcgggtgt agtgcgcgca tgtatagcag tcgcagtcgg cgtcgatggg
      661 tgtgaagtcg cggcggtagc gggcaccggt gatgttgtag cggccggtcg cggagtacac
      721 cgcggcgttg cgcgcgacgc gtgacggtga cacgcagtcg aacgtgtcgg cgccggcttc
      781 gaccgcatgg aacaggtcgt cgggctcgct gatccccagc agatggcgcg gcttgtctgc
      841 gggcagctcg ctgctgaccc agccgacgat cgtcgcgagg ttctgcttct ccagcgcacc
      901 gccgatgccg tagccgtcga agccgcggcc ctcgtcgtcg acgatggaca ccagcccgcg
      961 cgtcgcttct cgccgcagat cttcgtactg ggcgccctgc accacgccga agagggcctg
     1021 cgggtggcga tccgggtgag ccgcggtcat cctgcggtgt tcggccaggc agcgcaccgc
     1081 ccatcggtgc gtgcgctgca cggattcctc ctggtagccg cgggtgttca ccagggtggt
     1141 gagctcgtcg aacgcgaaga tgatgtcggc gccgatctgg tgctggatcc ggatcgacac
     1201 ctcgggcgtg aacctgtgag tcgatccgtc aaggtgcgac gtgaaggtca cgccgtcgtc
     1261 gtcgacgcgg gccagccgct ccttgccttc ggcgatgacg tcgtcggcct gcacccgctc
     1321 ggtgtccatc gccagtacct tgcggaagcc ggcgcccagc gacagcacct ggaatccgcc
     1381 gctgtcggtg aatgtcggac cgggccagtt catgaatgtg cccagtcccc ctgcctcatc
     1441 gatgatgtcg gcgccgggtt gcaggtacag gtggtaggcg ttggccagaa gagcttgtgc
     1501 accaagctct ttcatcgcct cgggaaggac ggccttcacc gtggcctgcg tgccgacggc
     1561 gatgaacgcg ggagtgtgga tgtcgccgtg cggtgttcgg atgacaccgg cacgccccaa
     1621 ccgccccgga atctccgctt cgacgctgaa agacggctcc
//
LOCUS       DASYLX010000586         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3209505,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000586 DASYLX010000000
VERSION     DASYLX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3209505"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP27_14010"
     CDS             complement(<1..351)
                     /locus_tag="VGP27_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852447.1"
                     /translation="METATALQLVDGVDLTGKTCVITGASAGLGRESARALAATGAHV
                     VLAARNRGALAETEEWIRADVPNAHTSTVQLDLTSLGSVRAAAATIVNITPVIHVLMN
                     NAGVMFTPFSRTSDG"
     gene            complement(351..518)
                     /locus_tag="VGP27_14015"
     CDS             complement(351..518)
                     /locus_tag="VGP27_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852448.1"
                     /translation="MSVKQRTSPTVSQFTMGIYSASLKCELREKLPLFGAQPLTSLGN
                     ACRGPIVSVQA"
     gene            complement(692..1258)
                     /locus_tag="VGP27_14020"
     CDS             complement(692..1258)
                     /locus_tag="VGP27_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7852449.1"
                     /translation="MRKTTQLRGEILAAARAEFAQYGLAGARIDRIARSAQASKERLY
                     AHFGDKEALFREVVAADVAEFFRAVTLRPDTVPDFVGNLYDLARARPEHLRMLTWARL
                     EGLSLDEPRADGEPIPAQTIAAIEAAQAGGHVDRAWQPRDLLVILFGIGLAWAHWPDP
                     HVTDDPTLVAHRRAAAVEAATRIVATPS"
     gene            1411..2280
                     /locus_tag="VGP27_14025"
     CDS             1411..2280
                     /locus_tag="VGP27_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7852450.1"
                     /translation="MNQPRRVRALRRVVETVGDGLGPLVDLYQPYVDGLQSLPRDGRF
                     LLVGNHTQSSAEVFLISYFVRRAIGTRVRPLAVRDMGRMRGAAGDLVAAYGAVVGAPE
                     TARELMRHNETILVFPGGGREIAKFKGEEHTLRWQGRSGFARLSAEANYSIVPVGLVG
                     GDDVYQSLTTREGRWGRLSQSLTAKLSGRTDMAVPLMHGIGPTLVPRPQRMYLRFAAP
                     VDTTKPTRVSEEKWVATVKQNTQESLEQTLADLLAIRNGDPYRGLNPLSWRTATQPPA
                     ELKGGVNHGSHIR"
     gene            2261..2641
                     /locus_tag="VGP27_14030"
     CDS             2261..2641
                     /locus_tag="VGP27_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7852451.1"
                     /translation="MAPTFAEVSKARYILLTTFTEDGKPKPTAVSVASDGDRLLVHTE
                     EDSWKVKRIRNTPRVTVAACDMRGRPKGEPVEAVAKVLDKAEAADVQRAITKRYGPIG
                     RLFDVYLKLRGVDKKSVGVEIMAV"
     gene            complement(2704..3468)
                     /locus_tag="VGP27_14035"
     CDS             complement(2704..3468)
                     /locus_tag="VGP27_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852452.1"
                     /translation="MGLLDKKVVLITGTGGGLGRVAAQTFAREGAKVVGSDIKVEANN
                     ETVELVRRTGGEMTGIAPIDLTDPEQARKMVEDAVAAYGGLDVVYNNAAIQHFGPMPD
                     FSVDDWRATISGELDIPFFVSKFAWPHLVRRGGGVIINVASVAGMIAAETPPIVGHAA
                     AKAGVIAMTRQFALEGAPHGIRAVAISPGPFLTPASDRDLGDDQAARDAITSKTLLKR
                     FGQPEEVVELAAFLASDRASYITGANYPVDGGATAW"
     gene            complement(3702..3875)
                     /locus_tag="VGP27_14040"
                     /pseudo
     CDS             complement(3702..3875)
                     /locus_tag="VGP27_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
     gene            complement(4248..5216)
                     /locus_tag="VGP27_14045"
     CDS             complement(4248..5216)
                     /locus_tag="VGP27_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEV7852453.1"
                     /translation="MADTRVLVTGGSGFIGSHCVAALLNADYRVRATVRSPEREADVR
                     DMVARAGANAAELTFAVADLMSDDGWPEAVGGSDFVLHVASPFPPDEPQDEEKVIAPA
                     CDGTIRVLRAARNAGVKRVVLTSSFAAIGYGHGQLDREFTEEDWTDIDGRGVTAYVKS
                     KVLAERAAWDFIKSGDGETELSVINPTATFGPTLSDDISASLIPILTLLTAGVAQMAN
                     FSFPVADVRDIADIHVRAMTHPGAANERFIACCDGSPITMGHAAYVLREWTGAETVIE
                     SPVLGEIVRPSNRKAKAVLGFNPRPIDEALLSTAGSLVRTGLVAAP"
     gene            complement(5299..>6816)
                     /locus_tag="VGP27_14050"
     CDS             complement(5299..>6816)
                     /locus_tag="VGP27_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEV7852454.1"
                     /translation="VWRDLWQRFGIALEVGFEQALALNMHIFHVLQVIVAADTDLDAG
                     LPARGLHGEGYRGHVFWDELYVYPLLTMRRPELTRCFLLYRYRRLPAARDLAWANGFD
                     GAMFPWQSGSDGRDETPGELFNLRNGQWMPDNSHRQRHVGLAVAYSAWQYYQATGYKG
                     FLEDYGAELIVQVARLFASMAVHDPVDDRFNISGVMGPDEYHDGYPGAPGLGLRNNAY
                     TNVLAAWVLARAVESVDLLIGRDCVPVWRRLDIRPGELEHWQRVSRRLRVPFHEGVIS
                     QFEGYEALQELDLDRYRERYGNIGRMDLILQAEGDSTNNYKLSKQADVLMLFCLFSAE
                     ELRELFNQMGYSLDPDLVQRTVKYYEARTTHGSTLSRVTHSWVSAQSDCAQSWSLFKE
                     ALKADLQDTQGGTTREGIHLGAMAGTADMVLRCYAGVETRDDVLHLHPVLPNEIGRAS
                     FEIIYRDQPVDIELTHQYARLRLLPCAADPIDVCVEGTKKTLGPGQSWAARLPAR"
BASE COUNT         1215 a         2197 c         2165 g         1239 t
ORIGIN      
        1 gccgtcagac gtccgactga acggcgtgaa catcaccccg gcgttattca tcaggacatg
       61 gatcaccggg gtgatgttga caatcgtcgc cgccgccgcg cgaacgctgc ccagcgaggt
      121 caggtcgagc tggaccgtcg atgtgtgcgc gttcgggacg tccgcacgta tccactcctc
      181 ggtctcggcg agtgcgccgc gattacgggc cgcaaggacg acatgcgctc ctgtcgcggc
      241 cagcgccctg gccgattctc ggcccaaccc agctgatgcg ccggtgatca cacatgtttt
      301 tccagtcaga tcgacaccgt caaccagctg gagcgcggtg gctgtttcca ttacgcctgc
      361 accgaaacga tcgggccgcg acacgcattg cccaagctcg tcagcggctg tgcgccgaac
      421 agcgggagct tttcgcggag ttcacatttc agcgacgcgg aatagatgcc catcgtgaac
      481 tgcgacaccg tcggcgatgt ccgctgctta acgctcatcc ggagccccgt cctgttgcgg
      541 ccacctcacg ctaccaagcc attgcagtta accgcaattg tgcagttata tgcaggttgc
      601 atgggttaat tccatgacgc aggctggaat caaaaggcac ccgtgaaggc cccagccccc
      661 cggaccgccc gcagttcgat accggcgctg ctcagctcgg ggtggcgacg atgcgggtgg
      721 cagcctcgac ggcggcggcg cggcggtgcg cgacgagggt ggggtcgtca gtcacgtggg
      781 ggtcgggcca gtgcgcccag gccaggccga ttccaaaaag gatgaccagc aggtctctcg
      841 gctgccaggc acggtcgacg tggccgccgg cctgggcggc ttcgatcgca gcaatggtct
      901 gggcggggat gggttcgccg tcggcacggg gttcgtcgag gctaaggcct tccaggcgtg
      961 cccaggtcaa catgcgcagg tgctcgggcc gtgcgcgggc caggtcgtag aggttgccga
     1021 cgaagtcggg gacggtgtcg gggcgcagcg tgaccgcacg gaagaactcg gcgacatcgg
     1081 cagcgaccac ttcgcgaaag agggcctcct tgtcgccgaa gtgggcgtag aggcgttcct
     1141 tgctcgcctg ggcggatctt gcgatgcggt cgatccgggc gcccgccagc ccgtactggg
     1201 cgaactcggc ccgggcggcc gccaggattt cgccgcgcag ctgggtcgtc tttctcacag
     1261 gagaatcata acaaacgaac tagttcgttt ggtagcgtgg cgacatcagt ctcaagcgat
     1321 gacatgccag gaggtttcga tgggtcaagt cgttgtagtg gacccggtga tcgcgctgga
     1381 tcggcggttg aggtgaacat cgacgccatc atgaatcagc cgcggcgggt ccgggcgttg
     1441 cgccgtgtcg tggagacggt cggcgatggg ctcggaccgc tcgtcgacct ctatcagccg
     1501 tacgtcgacg gcctgcagag cctgccccgc gatgggcgat tcctgttggt gggcaaccac
     1561 actcagtcca gtgccgaggt cttcctaatc tcgtacttcg tgcggcgggc gatcggcacg
     1621 cgggtgcggc ccctggccgt ccgggacatg ggccgcatgc gcggcgcggc cggcgatctg
     1681 gtcgctgcct acggcgctgt cgtcggggcg ccggagaccg cccgcgaact gatgcgtcac
     1741 aacgaaacga ttctggtgtt tcccggcggt ggccgtgaga tcgccaaatt caagggcgag
     1801 gagcacaccc tgcggtggca gggtcggtcc ggattcgccc gcctgtcggc ggaggccaac
     1861 tattcgatcg tgccagtcgg gctcgtcggc ggcgacgacg tttaccagag cctgaccacg
     1921 cgcgagggtc ggtgggggcg gctgagccag agcctcaccg cgaaactgtc cggtcgcacc
     1981 gacatggcag taccgctgat gcatgggatc ggtcccaccc tggttccccg gccgcagcga
     2041 atgtatctgc gcttcgctgc ccccgtcgac accaccaaac ccacacgggt gtcggaagag
     2101 aaatgggtgg ccaccgtcaa gcagaacacc caggaatcac tggagcagac cctcgccgac
     2161 ctcctggcca tccgcaacgg cgacccctac cgggggctga acccgctctc ctggcgcact
     2221 gcgacacagc cgccagccga actgaaaggc ggcgtgaacc atggctccca cattcgctga
     2281 ggtgtccaag gcccgctaca tcctgctgac cactttcact gaggacggca agcccaagcc
     2341 gaccgccgtc tcagtcgctt ccgacggcga ccgcctgctc gtccacaccg aggaagactc
     2401 gtggaaggtc aagcggatcc gtaacactcc gcgcgtcacc gtcgcggcgt gcgacatgcg
     2461 cgggcggccc aagggtgagc cggtcgaagc agtggcgaag gtccttgaca aggcggaggc
     2521 cgcggacgtg cagcgggcta tcacgaagcg ctacgggccc atcggccgac tgttcgacgt
     2581 gtacctaaag ctgcgcgggg tggacaagaa aagcgtcgga gtcgagatca tggccgtgta
     2641 gttgaggctc gggctggacc cggttttatc ccagagcgat ttttctttca agtgccctgt
     2701 gccctaccag gcggtcgcgc cgccgtcgac cgggtagttg gcgcctgtga tgtaagacgc
     2761 ccggtcagaa gcgaggaagg ccgcgagctc caccacctcc tcgggttgac cgaagcgctt
     2821 gagaagcgtc ttgctggtta tcgcatcccg cgccgcctgg tcgtcaccga ggtcgcggtc
     2881 gctggccggg gtcaggaaag gcccgggact gattgccacc gcgcggatcc cgtggggcgc
     2941 accttcaagt gcgaactgtc gggtcatggc gatgacgcca gctttcgctg cggcatgccc
     3001 gacaatcggg ggggtctctg cggcgatcat gcccgccacg gacgcgacgt tgatgatgac
     3061 accgccaccg cgccggacca ggtgcggcca tgcaaacttc gacacgaaga agggaatgtc
     3121 gagttcgcca cttatggtcg cccgccagtc atcgactgag aaatcgggca tcggaccaaa
     3181 gtgttggatg gccgcgttgt tgtagaccac gtcgagcccg ccgtaggcgg cgacggcatc
     3241 ctcaaccatc tttcgcgcct gttcggggtc ggtgagatcg atcggcgcga tgccagtcat
     3301 ctcgcccccg gtgcgacgta cgagttcgac cgtctcgttg ttggcttcaa ccttgatgtc
     3361 ggacccgaca acctttgcgc cttctcgcgc aaaggtctgt gccgcgacgc ggcccagacc
     3421 ccctccggtg ccggtgatca gcacgacctt cttgtcgagt aatcccatag ttcctaccct
     3481 ccgaactgtc tcgggccttc caaatcaggt gtggaatacg ccgctacggc agtaggacga
     3541 tcttgccggc actgcccgat ttcctcgcct tgcaggcccc ggcgataact gccaaacgcc
     3601 ctgttacaca atcggtccca gcatttcagc actctaccac gtcgctgcta tctactactt
     3661 ggccatgttg gtgaagcgcg acaggtgaag ttgatgcgcg ccggcacctc cagctccttc
     3721 gccaaaagct tgagctgacg ggagaattct gagacttcct gctggcgcga ctcgaccttc
     3781 ttgccagacg tcatcagctg taggtaatcg atgacgatcg acttcagatc gaacttctgc
     3841 ttgagctttc gtgcctccgc gcggatctcc atcatcgggt cgcgggcttg cagcgcgagg
     3901 aagtcgcttc gctggccggt gtcagcgtca actgctaaaa ccgcctggag cgggaccctg
     3961 gccacgtcaa catccgtcgg atgcgctaat catccctggc atccgagggg cggctgaccg
     4021 ggccgcaaga tcaccggatc gatgtcctcg ccgctcagct cgccggccgg aacctcgtgt
     4081 gctcgtggcc gcgtaccaag ccaatagcga acaggatgac ggccgaaaca tcatgcgcgg
     4141 ctgcgtaccc gggaggtcct gacacaaacg tcgcgcatcc gacgctgccc cgtcgcggcg
     4201 gatcaaatcg ggtgtccaag tgttgacatc ccagcacggg tcggttccta gggtgccgct
     4261 accagccctg tacggaccag gctgcccgca gtggacaaca gcgcttcgtc gatcggtcgc
     4321 ggattaaatc cgaggacagc cttagccttt cggttactgg ggcgcacgat ttcaccgagg
     4381 acgggtgact cgatgaccgt ttccgcgccg gtccactcgc gcaggacgta ggcggcatgg
     4441 cccatcgtga tcgggctgcc gtcacagcag gcaatgaatc gttcattcgc ggcgccggga
     4501 tgggtcatgg cccgcacgtg tatgtcggcg atgtcacgca cgtcagcgac aggaaaactg
     4561 aagttcgcca tttgcgccac accagcggtg agcagagtca gtatggggat cagcgaggcg
     4621 gagatgtcat cacttaacgt gggtccgaac gtggcggtcg gattgatgac actgagttcg
     4681 gtctcgccgt ctccgctctt gatgaagtcc catgccgcgc gttcggcgag caccttcgac
     4741 ttgacgtagg ccgtgacgcc tcggccgtcg atgtcggtcc agtcctcctc ggtgaactcg
     4801 cggtccagct gcccgtgacc gtagccgatc gccgcgaacg acgatgtgag cacaacgcgt
     4861 ttgacgcctg cgttccgggc agcgcgtagc accctgatgg tcccgtcgca tgcgggcgcg
     4921 attaccttct cttcatcttg gggctcatcg ggtgggaagg gggaggcgac atgcagcacg
     4981 aagtcgctgc cgccgaccgc ctcaggccat ccatcgtcgg acatgaggtc ggcaacggcg
     5041 aaagtcagct ccgcggcgtt ggcacccgca cgcgcgacca tgtctcgtac gtccgcctcg
     5101 cgttcgggtg atctcaccgt cgcgcggacg cggtaatccg cgttcagcag tgctgcgacg
     5161 caatgcgagc cgatgaatcc ggatccgccg gtgacgagaa cacgtgtgtc agccatgatt
     5221 tcccacatcg tttgaaaggt ttgacgctgt ccccgcccct gaggctcagg tgcggggtgg
     5281 cgatcttgtg gatcgcatct agcgggcggg tagacgcgcc gcccacgatt gcccgggtcc
     5341 cagggtcttt ttcgtgccct caacacacac gtcgatcggg tccgccgcgc acggcaggag
     5401 ccgcagccgc gcatattggt gggtcaactc gatgtcgacc ggctgatcgc ggtagatgat
     5461 ctcgaacgac gcccggccga tctcgttcgg cagcaccgga tggaggtgaa ggacgtcgtc
     5521 ccgtgtttcc acaccggcgt agcagcgcag caccatgtcg gccgttccgg ccatcgcccc
     5581 gaggtggatc ccttcgcgag tcgtcccgcc ctgcgtgtcc tgaaggtctg ccttcagggc
     5641 ttccttgaac agcgaccacg actgcgcgca gtcgctctgg gccgacaccc agctgtgtgt
     5701 aaccctgctc agcgtagagc cgtgagtcgt ccgtgcctcg tagtacttca ccgtccgctg
     5761 caccagatct gggtcaaggg agtaacccat ctggttgaac aattcgcgca gttcttcggc
     5821 ggagaacaaa cagaacagca tcaagacatc ggcctgcttg gacaacttgt agttgttcgt
     5881 ggagtcgcct tcggcctgca ggatcaggtc catccgcccg atgtttccgt agcgttcgcg
     5941 gtagcggtcc aagtcgagct cctgtagcgc ctcgtacccc tcgaactgac tgatcacgcc
     6001 ttcatggaaa ggcacccgca accgtcggct cacccgctgc cagtgctcaa gctctcccgg
     6061 cctgatgtcc aggcgacgcc acaccgggac acaatcgcgg ccgatcagca agtcgaccga
     6121 ctcgactgcc cgtgctaata cccaggcagc caacacattt gtgtaggcgt tgttgcgaag
     6181 acccagcccg ggggcacccg ggtagccgtc gtggtactcg tcgggcccca tcacgccgct
     6241 gatgttgaac cggtcatcaa ccggatcgtg aactgccatg ctggcgaaca ggcgagcgac
     6301 ctgcacgatg agctcggcgc cgtagtcctc gaggaagccc ttgtaaccgg ttgcctggta
     6361 gtactgccag gcgctgtagg ccaccgccag gccaacatga cgctgtcgat gggagttgtc
     6421 cggcatccac tggccattgc ggagattgaa cagctcgccc ggcgtctcgt cgcgaccatc
     6481 gctgcccgac tgccaaggga acatagcgcc gtcaaaaccg ttggcccaag ccagatcccg
     6541 cgcggccggt agcctacgat atcggtacag caggaagcag cgggtcagtt ccggccttct
     6601 catggtcagc aacggataga cgtagagctc gtcccagaag acgtgcccgc gatagccctc
     6661 gccgtgcaac ccacgcgcgg gcaggcccgc atcgagatca gtgtcggccg cgacgatcac
     6721 ctgcagcacg tggaagatgt gcatgttcag cgccaacgcc tgctcgaacc cgacctccag
     6781 ggcgatgccg aaccgctgcc acaagtcccg ccatac
//
LOCUS       DASYLX010000587         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2551182,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000587 DASYLX010000000
VERSION     DASYLX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551182"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VGP27_14055"
     CDS             <1..106
                     /locus_tag="VGP27_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852455.1"
                     /translation="LSTIPLQVFAAGVAQARGYDVDKPRNLAKSVTVE"
     gene            125..286
                     /locus_tag="VGP27_14060"
     CDS             125..286
                     /locus_tag="VGP27_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852456.1"
                     /translation="MSTISNESAGASSPSEGAPDAFLDFIEERYSQWEREFAEEVHVR
                     RTTTSNPKR"
     gene            complement(410..1984)
                     /locus_tag="VGP27_14065"
     CDS             complement(410..1984)
                     /locus_tag="VGP27_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEV7852457.1"
                     /translation="MAALTLLLVLMAATVVLAPVAERVSIPYPVLLLVFGLMLAFVPG
                     LPLPAVNPELLLPLVLPPLLFAAARRTSWREFLDNRRPIALLAVALVVVTAFAVGMTL
                     QALMPGLPLIAGLALGAAVAPPDPVAATAVARKLGLPHRLRTILEGEGLSNDATALVL
                     YEVAVAATLTGAFSPWRGASALGVAVVVGVGVGVIIAAIGRLLLKKLPAHPAGSALIL
                     LMPFAAYAAADAAHGSGVLAVVTVALALDRYGDADSSQTRLVAGTTWEIIELLVTGAA
                     FAFVGLELRAVADEADGQLDTMIGQAAIVTAVVIVVRFLWIFPAATIDEKIRRRRNND
                     LAEPVGWREMTVASWAGMRGVVTLVAALALPQNFPERDRLIFIAFVVISVTLLLQGLT
                     LPWLVRRLGVSGSDEERHRAEQELIRRARTAGFDRLDELREREEADGDVIEQARENAD
                     RMWHSLGYAPNGSDDGARRASDRAGDAVTVNAVKDEMLAAARDAVVRSRIQSGTDPTI
                     ADRVLRRLDARGSQPE"
     gene            2126..>3030
                     /locus_tag="VGP27_14070"
     CDS             2126..>3030
                     /locus_tag="VGP27_14070"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEV7852458.1"
                     /translation="MRYYYSADAIREAEAPLLASLPDGVLMRRAANGLANAIARELVV
                     RTGGVAGRSVCAVVGSGDNGGDALWAATFLRRRGVWAAAVLLDPERTHQEALTAFRKA
                     RGRVVQSVSATIDLVVDGVVGISGSGPLRPAAAEVFAAVDEAGIPVVAVDVPSGVDVN
                     TGAVSGPAVRPVLTVTFGGLKPVHALGECGRVELVDIGLDLPASDVMSFDAADVATRW
                     PMPGPKDDKYTQGVTGILAGSSTYPGAAVLCTGAAVAATSGMVRYAGSAAQQVLSHWP
                     EVIASPSPAAAGRVQAWAVGPGLGV"
BASE COUNT          510 a         1048 c         1024 g          448 t
ORIGIN      
        1 cctgtccacg atcccgttgc aggtgttcgc cgccggggtg gcgcaggccc gcggctacga
       61 tgtggacaag ccgcgcaacc tggccaaatc ggtcaccgtc gagtagcgaa tggaggctct
      121 gacgatgagc acgatatcca atgaatctgc tggtgcttcc agtccgtcgg aaggcgctcc
      181 cgatgcattc ctggacttca tcgaggagcg ctacagccaa tgggagcgtg agttcgccga
      241 agaggtgcat gtgagacgca cgacgacgtc caatcccaag cgttagaagt gcggccaaac
      301 ctgacctgac ttgaaaccgc aaaccgccgc cgtttccggc ggtggcgccg ccgatctctc
      361 cggtgccggt ttcttcgatg attgagcgga aacggtcgga gcgtcagacc tattcgggct
      421 ggctgccgcg cgcgtcgagc ctgcgcagca cgcggtcggc gatggtcggg tcggttccgg
      481 actggatgcg gctgcgcacc accgcgtccc tggcggccgc caacatctcg tccttcaccg
      541 cattgaccgt gaccgcatcc ccggcgcggt ccgacgcgcg ccgcgctccg tcgtcgctgc
      601 cgttcggcgc gtagcccagc gaatgccaca tccggtcggc gttctccctt gcctgttcga
      661 tgacgtcgcc gtcggcctct tcccgctcgc ggagttcgtc gagtcggtca aaacccgcgg
      721 tcctcgcgcg tcggatcaat tcctgctccg cccggtgccg ctcctcatcg gaaccactca
      781 caccgaggcg ccgcacaagc cacggcagcg tcagaccctg caggagcagc gtgacgctga
      841 tgacgacgaa cgcgatgaag atcagccggt cgcgctccgg gaaattctgc ggcagggcca
      901 gtgcggccac cagggtcacg acgccgcgca tccctgccca cgacgcgacg gtcatctcac
      961 gccagccgac aggttcggcg agatcgttgt tgcggcgccg gcgaatcttc tcgtcgatgg
     1021 tggcggccgg aaagatccac aggaaacgga cgacgatcac cacggccgtc acgatcgcgg
     1081 cctgaccgat catggtgtcc agttgcccgt ccgcctcgtc ggccaccgcg cgcagctcga
     1141 ggccgacgaa cgcgaaggcc gcaccggtca ccagcagttc gatgatctcc caggtcgtac
     1201 ccgccaccag ccgggtctgc gaggagtcgg cgtcgccgta ccggtccaac gccagggcga
     1261 cggtgacgac cgcgagcaca ccactgccgt gagcggcgtc ggcggcagcg taggcggcga
     1321 acggcatgag caggatcagc gcgctccccg cggggtgagc gggcagcttc ttcagcagga
     1381 gtcgaccgat ggcggcgatg atgaccccga cccccacgcc gaccaccacc gcgactccca
     1441 gcgccgatgc accgcgccac ggcgagaacg cacccgtcag ggtcgctgcc accgccacct
     1501 cgtagagcac caacgcagtc gcgtcgttgg agagcccttc cccctccaag atcgtccgca
     1561 accggtgcgg aagccccagc ttgcgggcga ccgcggtggc ggcaacgggg tcgggcggcg
     1621 cgaccgccgc accgagagcg agcccggcga tcaggggcag acccggcatc agggcctgca
     1681 gggtcatccc caccgcgaat gccgtcacca ccaccaacgc gactgccagc agagcgatcg
     1741 gtctgcggtt atccaggaac tcgcgccacg aagtccggcg ggcggcggcg aacagcagcg
     1801 gcggcagaac cagcgggagc agcagttccg gattgacggc cggcagcggc aaaccgggaa
     1861 cgaacgcaag catcaggccg aagaccaaca gcagaacggg ataggggatc gagacgcgct
     1921 cagcaaccgg cgcgagcacc accgtcgccg ccatcagcac cagtagcagt gtcaaggcag
     1981 ccaagcggca acatctccca ttctccccgg cgtgtgtgaa ctgcctgggt ggattcccgt
     2041 tatcgccggt ctgaatccga ccgggatata acgtgggagt gatccgcgtc gcagcgcggc
     2101 gggcaaaccg acgggacggg agctgatgcg gtactactac tccgctgatg cgatccgcga
     2161 agccgaagca ccgctgctgg catcgttacc cgacggcgtc ctgatgcggc gggctgccaa
     2221 cggactggcg aacgccatcg ctcgcgagct cgtggtgcgc accggaggag tcgcgggccg
     2281 ttccgtctgt gccgtggtcg gctccggcga caacggcggt gacgcgctgt gggcggcgac
     2341 gttcctgcgc cgccgcggag tgtgggccgc ggccgtcctg ctcgatcccg aacgcaccca
     2401 ccaggaagcg ttgacggcgt tccggaaggc gcgcggtcgg gtggttcaaa gcgtttcggc
     2461 gacaatcgat ttggtcgtcg acggtgtcgt cggtatctcc ggatccggac cgctgcgacc
     2521 ggccgccgca gaggttttcg ccgccgtcga cgaggcagga atccccgtgg ttgccgtcga
     2581 cgttccgagc ggggtcgacg tgaacaccgg cgcggtcagc ggacccgccg tacgccccgt
     2641 gttgaccgtc accttcggcg gcctgaaacc cgttcacgcg ctgggcgaat gcggcagggt
     2701 cgaactagtg gacatcggcc tcgacctgcc tgcgtccgac gtcatgagtt tcgacgcggc
     2761 cgacgtcgcg acccggtggc caatgccggg gccgaaggac gacaagtaca cccagggcgt
     2821 caccggcatc ctggccggct cgtcgacgta cccgggcgcg gccgtgctgt gcaccggcgc
     2881 cgcggtggcc gccacctccg gtatggtccg ctacgcggga agtgctgcac agcaggtgct
     2941 ttcgcattgg cccgaggtca tcgcgtcgcc gagcccggcc gctgcgggcc gggtacaggc
     3001 gtgggccgtc ggtcccggtc tgggcgtcga
//
LOCUS       DASYLX010000588         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1234712,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000588 DASYLX010000000
VERSION     DASYLX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234712"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGP27_14075"
     CDS             <1..214
                     /locus_tag="VGP27_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV7852459.1"
                     /translation="VVVPNLLLVNKDMDGNTACVLTKALFDHKADLVKVNKAAKDINL
                     DTARQTDPVPLHPGAKKALDELGAKN"
     gene            325..2292
                     /locus_tag="VGP27_14080"
     CDS             325..2292
                     /locus_tag="VGP27_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEV7852460.1"
                     /translation="MTGWVSHAVTAVCVLIGVSAIYQVFFPLAQGNQVSLIYFLAAVL
                     PLTLLCYRSGVRLRRRTEETDEHDEREVPTGNPTPVDWLLAAIALVACLYPVLPFEIA
                     GGGGGFDEFLSRQGTPAMIDVVVGTVVLLLVLEACRRTTGLALPIVCLVALAYAYYGG
                     YLPQGAAIAHAGVNWDQIISASFIQSTGLYGVPLDVAATYIVLFTIYGAVLDASGASK
                     FFIDLSLAAFRRSRAAPGRTVTLAGFLLGSVSGSGTATTVSLGSVTWPILRRAGYPKE
                     NAGGMLAAAGIGAILSPPTLGAAAFIIAEYLGVPYLTVLLWAIVPTILYYLGILLAVE
                     IDARRYGTREVEISEQSGWRLLGRFGYHLISLLLIVVFLAMDISAVRAVVFATFIQIA
                     LSFLDREHRLTPIRLYRALGTGVRGVLAVATVCAAAGVIAAMITRTGLGLQLNSILVN
                     AARGITENPALVLVFTALFAALAVAVLGLAVPVTASFVISWVIVGPALQDLGVAAPAV
                     AMFVFYFAVLSEVTPPTALASVAASAITGGRVMATMMQTWKYTLPAFLVPFAFVLTDN
                     GEGLLGLRSIGDMAWTAAVSALGVAALAVVTGGWLIRRAGWPERVLCAPAAALLLYLH
                     PTTIVAGAVLLGVALGLNLLLRREAPTPQPA"
     gene            complement(2280..2738)
                     /locus_tag="VGP27_14085"
     CDS             complement(2280..2738)
                     /locus_tag="VGP27_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7852461.1"
                     /translation="MAKRRSIRNSRALVLLMKYFARAHIWVYQRTNGRLGAKLLRFPA
                     ALITTKGRKTGEPRITPTLYLRDGDRVLLPASFGGRDTDPGWYHNLKANPSVHIQIRD
                     EHLDLTARDATDEERRHYWPRLIEMYPPYRGYREAADRLIPLVVCERQAG"
BASE COUNT          413 a          905 c          920 g          541 t
ORIGIN      
        1 ggtcgtcgtg ccgaatcttc ttctggtgaa caaagatatg gacggcaata cggcatgtgt
       61 gctgacgaag gcgctgttcg atcacaaggc cgatctggtc aaggtgaaca aggcggccaa
      121 ggacatcaat ctggacacgg ctcgccagac cgaccccgtg ccgttgcacc cgggggccaa
      181 gaaggcgttg gacgaactcg gcgcgaagaa ctgaccgaag ccggcgacaa cgacgacggc
      241 cggcccgagc cggccggtgg taccgcgacc aagacagagc ctgcggccgc tgccgcctac
      301 gaagaagaga agcccgcccg cgagctgacc ggctgggtca gccacgcggt caccgccgtg
      361 tgcgtcctca tcggcgtgtc cgcgatctat caggtcttct ttccgctcgc gcagggcaac
      421 caggtctcgc tgatctactt ccttgcggcg gtgctcccgc tgaccctgct gtgctaccgc
      481 tccggagtcc ggctgcgtcg gaggacggaa gagaccgacg agcacgacga gcgcgaggtg
      541 ccgaccggca atccgacccc ggtggattgg ttgctcgccg cgatagcgtt ggtggcctgc
      601 ctgtatccgg tgcttccttt tgaaatcgcc ggtggtggag gcggtttcga cgagttcctc
      661 agccggcagg gcacccccgc gatgatcgat gtggtggttg ggaccgtcgt gctgcttctg
      721 gtgcttgagg cctgtcgtcg aaccaccggc ctcgcgctgc cgatcgtgtg cctcgtcgca
      781 ctggcctacg cctattacgg cggttatctg ccgcagggcg cggcgatcgc gcatgcgggt
      841 gtgaattggg accagatcat cagcgcctcg ttcatccagt caaccggcct ctacggtgtg
      901 ccactcgacg tggcggccac ctacatcgtt ttgttcacca tttatggagc ggtgcttgac
      961 gcgtcgggcg ccagcaagtt cttcatcgac ctctcccttg cggcgttccg gcggtcccgc
     1021 gcggcgccgg gccggacggt caccctggcg ggattcctgc tgggctccgt atcggggtcg
     1081 ggcaccgcga ccaccgtcag cctgggctcc gtgacgtggc ctatccttcg ccgggccggc
     1141 tacccaaagg agaacgcggg cggaatgctg gcggccgcgg gcatcggggc gatcctgtcc
     1201 ccgccgacgc tcggcgcggc ggcattcatc atcgccgagt acctcggcgt gccgtatctg
     1261 accgtgctgc tttgggcgat cgtgccgacg atcctgtact acctgggcat cctgcttgcc
     1321 gtcgagatcg acgccaggcg gtacggcacg cgcgaagtcg aaatcagcga gcagtcgggt
     1381 tggcggctcc ttggcagatt cggataccac ctgatctcgc tgctgctgat cgtggtcttc
     1441 cttgcgatgg acatctcggc ggtgcgcgca gtggtcttcg caaccttcat ccagatcgcg
     1501 ctgtcgttcc tggatcggga acaccgcctg acgccgatca ggctctaccg cgcgctgggc
     1561 accggcgtca ggggtgtgct cgccgtagcc accgtgtgcg ccgccgccgg cgtcatcgcc
     1621 gcgatgatca ctcggaccgg gctcggcctg cagctgaact cgattctggt caatgcagcc
     1681 cggggcatca ccgaaaaccc tgctcttgta ctcgttttca ccgcgctgtt cgccgcgctg
     1741 gcggtggcgg tactcggcct ggccgtgccg gtgacggcct cgttcgtgat cagctgggtg
     1801 atcgtcggcc cggcgctaca ggacctcggc gtcgccgctc cggcggtggc catgttcgtc
     1861 ttctacttcg cggtgctgtc ggaggtgacc ccgccgacgg cgttggcatc ggtggccgca
     1921 tccgcgatca ccggcggccg ggtgatggcc accatgatgc agacgtggaa gtacacgctg
     1981 cccgcgttcc ttgtgccgtt cgccttcgtg ttgacagaca acggtgaagg cctgctcggg
     2041 ctgcgatcaa tcggcgacat ggcttggacg gcagcggtgt ccgcgctcgg tgtggcagca
     2101 cttgcggtcg tcaccggcgg ctggctgatc cggcgcgcgg gttggccgga gcgggtgctg
     2161 tgcgcaccgg ccgccgcact gttgctgtat ctgcacccga ccacgatcgt cgccggtgcg
     2221 gtcctcttgg gggtcgcgct agggctgaac ctgctgctgc gccgggaagc gccgacgcct
     2281 cagcccgcct gacgctcaca gaccacaaga ggtatcagcc gatcggccgc ctcgcggtag
     2341 ccgcggtacg gcgggtacat ctctatcaat cgcggccagt aatgccgtcg ttcttcgtct
     2401 gtcgcgtcgc gggcggtcag atcgaggtgc tcatcgcgaa tctgaatgtg cacgctgggg
     2461 ttcgccttca aattgtgata ccaccccggg tcggtgtccc gaccgccgaa cgatgcaggc
     2521 aacaagaccc ggtccccgtc gcgcaggtac agcgtggggg tgattcgcgg ttcgccggtt
     2581 tttcggcctt tcgtcgtgat cagcgctgcg gggaaccgga gcaacttggc gccgagacgc
     2641 ccgttcgtgc gctggtagac ccagatgtgt gcgcgcgcga agtacttcat caacaggacc
     2701 aacgcacgcg agtttctgat cgaccgacgc ttcgccatga cctatttctt gccacaaccg
     2761 cacatgggca aactatttc
//
LOCUS       DASYLX010000589         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2882127,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000589 DASYLX010000000
VERSION     DASYLX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882127"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VGP27_14090"
     CDS             complement(<1..1206)
                     /locus_tag="VGP27_14090"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7852462.1"
                     /translation="MTGDMALPPTLAANPRLGDWLRIGSDGTVEVHSGKVELGQGILT
                     ALAQMVAEELDVDVGRIRMVATATGLSPDEGMTAGSLSIQQSGAALREVCAEARDLYL
                     AIAADKLAVPKEDLAVDDGRITNRDGTATSYWELSDESVLDRPASGGAKPKPESDYHV
                     VGTDVARIDLPDKLFGRPRYVHDLQFDGMLYGRVVRPPSRGAMLNALDERAALALPGV
                     VTVVSDGDFLAVVAEREEAALRAADRLRADAVWEQKPSLPDEDDLPRFLTEAPTDTTV
                     IADGPRSTSAARRSVSATYHRPFIAHGSIAPSSAVALARDSELEIWSHSQGVHILARE
                     IARELGMRPEQLVVRHVEGAGCYGHNGADDAAMDAALLALAVPGRHVQVVWSRADELS
                     WAPLGTAGVV"
     gene            complement(1203..>1578)
                     /locus_tag="VGP27_14095"
     CDS             complement(1203..>1578)
                     /locus_tag="VGP27_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7852463.1"
                     /translation="GTRFGCGLGVCGACLVLVDAEPAYACDTPMWSVDGKDVTTVEGL
                     GAGGEPHAVARSIVAQQAAQCGYCMSGIVVRAAHLLAENPDPSEADVRAALDDNLCRC
                     GSHNRVVRAVLSAAAQMREGAT"
BASE COUNT          235 a          573 c          526 g          244 t
ORIGIN      
        1 gacgacgccc gccgtcccca gcggcgccca cgacagttcg tcggcgcgcg accacaccac
       61 ctggacgtgc ctgcccggca cggcaagtgc cagcagtgcc gcgtccatcg cggcgtcgtc
      121 ggccccgttg tggccgtaac atccggcacc ctcgacgtgg cggaccacca gttgctctgg
      181 gcgcattccc aactcgcgcg cgatctcccg cgcaagaatg tgcacaccct ggctgtgcga
      241 ccagatctcc agttcgctgt cgcgtgccaa cgcaaccgca ctggacggtg cgatcgaccc
      301 gtgtgcgatg aacggccgat ggtaggtggc cgacaccgac cgccgcgccg cgcttgttga
      361 tcgcggaccg tcggcaatca ccgtggtatc ggtgggcgcc tcggtcagga accgcgggag
      421 gtcatcttcg tccggcagag agggcttctg ctcccagacg gcgtcggcgc gcagcctgtc
      481 tgccgcccgc agtgcggcct cctcgcgctc ggcgaccacg gcgaggaagt cgccgtcgct
      541 caccacggtg acgacccccg gcagcgcgag agcggctctt tcgtcgagtg cgttgagcat
      601 cgcgcctcgc gacggtggcc gcaccacccg gccgtacagc atgccgtcga actgcagatc
      661 gtgcacgtaa cgtggtcgcc cgaaaagctt gtccggcagg tcgattcgtg ccacgtcggt
      721 gcccacgacg tggtagtccg actccggttt aggtttggcg ccaccgctgg cgggccggtc
      781 cagcaccgac tcgtcggaca gctcccagta actggtcgcc gtgccgtcgc gattggtgat
      841 ccgaccgtcg tcgacggcga gatcctcctt ggggaccgca agcttgtccg cggcgatcgc
      901 gaggtagagg tcgcgcgcct ctgcgcagac ctcacgcagc gcggcgcctg actgctggat
      961 ggacaggctg cccgccgtca tcccctcgtc cggactgaga cctgttgccg tcgctaccat
     1021 ccggatccgg cccacgtcga cgtcgagttc ctcggcgacc atctgtgcca gcgcggtgag
     1081 gatgccctga ccgagctcga ccttgccgga atgtacctcc acggtgccgt cggacccgat
     1141 ccgcaaccag tcaccgaggc gcgggttggc cgcaagcgtc ggcggtagcg ccatgtcgcc
     1201 ggtcacgttg cgccctcgcg catctgggcc gcggccgata acacggcgcg gaccacgcgg
     1261 ttgtgggagc cgcagcggca caggttgtcg tcgagagcgg cacgcacgtc ggcctccgac
     1321 gggtccgggt tctcggcgag cagatgcgcg gcgcgcacca cgatcccgga catgcagtag
     1381 ccgcactgcg cggcttgctg cgcgacgatc gaacgggcga ccgcatgggg ttcgccgccg
     1441 gcgccgagac cttcgacggt cgtcacgtcc ttgccgtcga ccgaccacat cggggtgtca
     1501 cacgcgtagg caggctctgc gtcgaccagc accaggcagg cgccacagac gccaagcccg
     1561 cagccgaacc gggtgccc
//
LOCUS       DASYLX010000590         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_139279,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000590 DASYLX010000000
VERSION     DASYLX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139279"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGP27_14100"
     CDS             <1..710
                     /locus_tag="VGP27_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV7852464.1"
                     /translation="IPSVTTVQMEDFAHVMRRLWNGEVIFNHDGPIGKYQVLFLDPDF
                     KEVIRLALVAFGPKTLELGGRVFDDVILHTYFTPETLQRCVNTVKDAAQQAGRDPDSV
                     RVWSCFATVGDHLPEELRLKKTVARLATYLQGYGDLLVHTNNWDPAVLQRFREDSVVT
                     SIPGGIDHKATAQQIEHIATLIPDEWLEPSATGSAQQCVDRIRKEFAYGADALIMHGA
                     TPDELKPIVTAYRSATA"
     gene            complement(700..>1696)
                     /locus_tag="VGP27_14105"
     CDS             complement(700..>1696)
                     /locus_tag="VGP27_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7852465.1"
                     /translation="VAPVCDFTIMSRQGAIFTAGPPVVKESTGEDISKEDLGGPSVAL
                     ASGVIHNLAEDDKSVLDDIRRYLAYFPSSAWSYPPVAPADESSAPRPTPELLDIVPRT
                     NRRVYDIRTVLDVVFDRQDWFEVQPKFGPAIICALAHLGGHPVAVVANQPKVMAGSID
                     ADAADKAAHFITVADSFHLPIVFLADNPGMLPGSRSEKSGVLRSGARMFAAQTAATTI
                     KLHVTLRKAYGFGSMVMSLLGFDSQSATFAYPGATMGAMSAAALSRASHAAEDVEAQL
                     RKAEVEASFRSAGHLGFDDLIHPEETRDALLSALQRAVYSRQAAAEPVSRTVITP"
BASE COUNT          288 a          569 c          555 g          284 t
ORIGIN      
        1 gcattccgtc ggtgacgacc gtccagatgg aggacttcgc gcacgtcatg cgtcggctct
       61 ggaacggcga ggtcatcttc aaccacgacg ggccgatcgg taagtaccag gtgctgttcc
      121 tcgatccgga cttcaaggag gtcatcaggc tcgcgctggt ggccttcggc ccgaaaaccc
      181 tcgaactggg cggccgggtg ttcgacgacg tcatcctgca cacctacttc acgccggaaa
      241 ccctgcagcg ctgcgtcaac accgtgaagg atgccgcaca gcaggccgga cgcgatcccg
      301 acagcgtgcg ggtgtggtcg tgcttcgcga cggtcggcga tcacctcccc gaagagctac
      361 ggctgaagaa gaccgtcgcg cggctggcca cctacctgca gggctacggc gaccttttgg
      421 tccacaccaa caactgggat cccgctgtgc tgcaacggtt ccgcgaagac agtgtcgtca
      481 cctcgatacc aggcggcatc gaccacaaag ccaccgccca gcagatcgaa cacatcgcga
      541 cgctgattcc ggatgagtgg ctcgagcctt cggccaccgg atcggcgcaa cagtgcgtcg
      601 accggatccg caaggaattc gcctacggcg cggacgcgtt gatcatgcac ggcgcgacgc
      661 cggacgaact caagccgatt gtcaccgctt atcgctctgc tacggcgtga tgacggtgcg
      721 gctcaccggt tccgccgcgg cctgcctgct gtagacggca cgctgcagcg ccgacaacag
      781 cgcatcgcga gtctcctccg gatggatcag gtcgtcgaac ccgaggtggc ccgccgaccg
      841 gaaggacgcc tccacctccg ccttgcggag ctgggcctcg acgtcctcgg cggcgtgcga
      901 cgcacggctg agcgcggccg cgctcatcgc tcccatcgtg gcgccgggat aggcgaacgt
      961 cgcgctctgg ctgtcgaacc ccagcagcga catcaccatt gagccgaacc cgtaggcctt
     1021 tcgcagcgtc acgtgcagct tgatcgtcgt cgcggccgtc tgcgcggcga acatccgtgc
     1081 gccgctgcgc agcacgccgc tcttctccga ccggctgccg ggcagcatcc ctgggttgtc
     1141 cgcgaggaac acgatcggca ggtggaacga gtcggcgacc gtgatgaaat gcgctgcctt
     1201 gtcggcggcg tcggcgtcga tcgagcctgc catgactttc ggctggttcg cgacgacggc
     1261 gacggggtgg ccgccgaggt gagccagcgc gcagatgatc gcgggcccga atttcggctg
     1321 cacctcgaac cagtcctgtc gatcgaagac cacgtcgagc accgtgcgaa tgtcgtacac
     1381 ccggcggttc gtgcggggaa cgatgtcgag cagctccggt gtgggtcggg gcgcgctgct
     1441 ctcgtccgcg ggcgcgacgg gtggatacga ccatgcgctg gacgggaagt acgccaggta
     1501 gcggcggatg tcgtcgagca cgctcttatc gtcctcggcg aggttgtgga tcacaccact
     1561 ggcgagcgcg acgctcggcc cgcccaggtc ctccttcgaa atgtcctcgc ctgtggactc
     1621 tttgaccacc ggcggacccg cggtgaagat ggctccttga cgactcatga tggtgaagtc
     1681 gcacaccggc gcgacc
//
LOCUS       DASYLX010000591         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1784823,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000591 DASYLX010000000
VERSION     DASYLX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784823"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGP27_14110"
     CDS             complement(<1..374)
                     /locus_tag="VGP27_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852466.1"
                     /translation="MIVLLLFAVLAGAATAVTPCVLPVLPALLSASGTGGRRRPLGIV
                     AGLTLTHTITIVGIASVVDGVGLADGTLRTVAIVVLALFGVTLLVPPAGHWIAERMSG
                     LARLSPRSAGRGFWSGLPVGGAL"
     gene            415..1002
                     /locus_tag="VGP27_14115"
     CDS             415..1002
                     /locus_tag="VGP27_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852467.1"
                     /translation="MTLSVACSLVCAPMRKRVVSIITRPVRLARRLVELLVRLLRTAF
                     TKLAGLRGRQRIVAFGVLGVVIVVLVLSLRPGPNSDKEVRATLDRYALATRAKDYQTL
                     CDDLYASELTDRIRSAGLPCEVALRTGLENRRNPQLKVLGVEVNGDQALARTRTTAIG
                     EPPSVDTIRMIRQGGHWRVASLSEPGAQLSRDGSP"
     gene            complement(1203..1391)
                     /locus_tag="VGP27_14120"
     CDS             complement(1203..1391)
                     /locus_tag="VGP27_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852468.1"
                     /translation="MRIAAPSAVLMSIAGSYIQEPVRTNSSTVPWRLRAAARCLDVSD
                     AANRETGWTARLGVAPVV"
     gene            1787..2059
                     /locus_tag="VGP27_14125"
     CDS             1787..2059
                     /locus_tag="VGP27_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852469.1"
                     /translation="MGRHSSDESHVLVGGSQRFIDDFEAGLGRSQDDMIAAWLEEWAG
                     HQDRDPDGGLHIGDWIPAQLAHVVGPERAEAALSASRLRRWDPPAQ"
     gene            2248..>3377
                     /locus_tag="VGP27_14130"
     CDS             2248..>3377
                     /locus_tag="VGP27_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852470.1"
                     /translation="MRIEVPRWGMTALVATLAIAGLGRGAAGAQAAGGTAKPPKGAIA
                     PFVTPTLPDPAFSVDPALIHGFDVTGFIQDMRANATNAGCPDETDPGRFGGTVTLNDQ
                     TVTIPCNLVVQMPANTLTWADFVHGGDPSFGDLSLGKGFASFEIGVVGNVVDGTYIAA
                     LAFVSQQSLNGGSGVIRSIDYTTGELHVDTGDPAHPATVVINDPKGRFGRAQSPDARF
                     SVDDANPTIHAATGYPMCVPRVHTDPTLALATENDPLCPQANRPRPSCRNFSIAGVAP
                     PASGELSPAPAGQRYCSQYVMPRPGTAGGPDPTQQAPFEVGDYIQYSGTLVNGANGAY
                     VSAHTIEANVGIYTQPGTQPSYMAIGEFGVGTADPNATAANGA"
BASE COUNT          529 a         1147 c         1176 g          525 t
ORIGIN      
        1 agcgcgccgc cgaccggcag ccccgaccag aagccccgcc ccgcgctgcg cgggctcagg
       61 cgcgcgaggc ccgacatccg ctcggcgatc cagtggccgg cgggcggcac gagcagcgtc
      121 accccgaaga gcgcgagcac gacgatcgcg acggtccgca gggtgccgtc ggcgaggccg
      181 acgccgtcga cgacggacgc gatgccgacg atcgtgatcg tgtgcgtgag cgtcagcccg
      241 gcgacgatcc cgagcggccg ccggcggccg cccgtgccgc tcgcggacag cagcgccggc
      301 agcaccggca gcacgcacgg cgtgacggcg gtggcggcgc cggccaggac tgcgaagagg
      361 aggaggacga tcatccgcgc agcgcgtgtt ctcgcattcg ctgcgtacgg atgcgtgacg
      421 ttgtcggtgg cctgttcact agtctgcgcg cccatgcgca agagagtcgt ctccatcatc
      481 acgcggccgg tgcgcttggc gcgccggctt gtcgagctgc tcgtgcggct gctccgcacg
      541 gcgttcacga agctcgcagg gcttcgcggc cggcagcgga tcgtcgcctt cggcgtcctc
      601 ggcgtggtga tcgtcgtcct cgtgctctcg ctccggccag gcccgaactc cgacaaggag
      661 gtgcgcgcga cgctcgaccg ctacgccctg gcgacgcggg ccaaggacta ccagacgctc
      721 tgcgacgacc tctatgcgag cgagctcacc gaccggatcc gcagcgctgg gctgccttgt
      781 gaagtcgcgc tgcgtaccgg tctggagaac cgccggaacc cgcagctgaa agtgctggga
      841 gtcgaggtca acggcgatca ggcgctcgcg cggacacgga cgacagccat cggcgagccg
      901 ccgtctgtcg acacgatccg gatgattcgc cagggcggcc attggcgcgt cgcgtcgttg
      961 tcagagcccg gagcgcagct ctcgcgcgac ggatcgccgt aggtcgccgt caggctcagc
     1021 agcggtcgag attgcctggt tgcccgtagg atcgcctgtc aggccagtcg acaggaaacg
     1081 acgcgtatgc cccgttcgcg tgcgaatgtg ttgaggctga tcgtcgcact ggtcctgatc
     1141 agcggcgcac aaccagcact gttcgccgga gtgccttcgg cgtttgccgg tgcggtagcg
     1201 gttcacacga ccggggccac gcccagccgc gcggtccatc ccgtctcacg gtttgccgcg
     1261 tcgctgacat cgagacagcg ggcggcggct cgcaacctcc atggaacggt ggacgagttc
     1321 gtccgcaccg gctcctggat atacgagccg gcaatcgaca tcaggactgc ggacggagcg
     1381 gcgatccgca tccacctcct ccacggatcc tgatcctcgc tggcctcgga tcatgagcct
     1441 gcacgaccgc ccgtccgcgg ccagcggctt cagagcggtc tttgcggagg ttcgatccat
     1501 acggctcgcc gtttcagccg atggtgcctg ctcgcttgct cgtctacgcc gatccggcgg
     1561 aggccaaccc gtggggattg aacgacgcgg accttggttc cttggtcgcg gctgccgacg
     1621 atcgcgatct cgaacgcgtc ttcgtctcgc tggccgaccc agagcacagt gagctagacg
     1681 aaggtcatta cgtcctcagt ccgctgagtg tggagagcta cacgcgaaca catcccaccg
     1741 aagcctggcc gatcgtcccg cacgccatct atccgccgag cggacattgg ggcgccacag
     1801 ctccgacgag tcgcacgtcc tcgtcggtgg ctcgcagcgc ttcatcgacg atttcgaggc
     1861 cgggctggga cgaagccagg acgacatgat cgctgcgtgg ctcgaagagt gggccggcca
     1921 tcaggatcga gaccccgacg gcggcctgca catcggcgac tggatccctg ctcagctggc
     1981 gcatgtcgtc gggcccgagc gcgcggaggc ggcgctcagc gcatcgcggt tgcggcgctg
     2041 ggatccaccg gcgcaataga cgaccaccgg cgcgagcgcc gaacgcgatc tgtcctccgt
     2101 ccggcgggca ggcggtccgc tggtcgcacc acctggtgtg cgcgaagcgg cgcggcgtcg
     2161 agcacgctcc gctcttagtc cgcgcctgtc atgcacctac ggtccctggt gttcggaagg
     2221 cagaccgacg atggcaagga gcgccggatg aggatcgagg taccacggtg ggggatgacc
     2281 gcgctggtcg cgacgctcgc gatcgctggt cttgggcgtg gcgccgcggg cgcgcaggcc
     2341 gcgggcggga cggcgaagcc accgaagggt gcgatcgcgc cgttcgtgac gccgacgttg
     2401 ccggatccgg cgttctcggt cgatccggcg ttgatccatg gttttgacgt gacgggtttt
     2461 atccaggaca tgagggccaa cgcgacgaac gcgggttgtc cggatgagac cgatccgggt
     2521 cgcttcggtg ggacggtgac gctgaacgac cagacggtca cgattccgtg caacctcgtc
     2581 gtgcagatgc cggccaacac gttgacgtgg gcggatttcg tgcatggcgg cgatccgtcg
     2641 tttggtgatc tgtcgctggg caaggggttc gcatcctttg agatcggggt ggtgggcaac
     2701 gtcgtcgacg gcacgtacat cgcggcgctg gcgttcgtgt cccagcagtc gctcaacggc
     2761 ggcagcggcg tgatccgcag catcgactac acgaccgggg agttgcacgt cgacaccggt
     2821 gacccggcgc atcccgcgac cgtggtgatc aacgatccca agggccgctt cggtcgcgcg
     2881 cagtcgcctg atgcgcgctt cagcgtcgac gacgcgaacc cgacgatcca cgccgccacc
     2941 ggctatccga tgtgcgtccc gcgggtgcat accgatccga cgctggcgct ggcgaccgag
     3001 aacgatccgc tgtgcccgca ggccaaccgt cccaggccgt cgtgtcggaa cttcagcatc
     3061 gccggtgtcg cgccgcccgc gagcggtgag ctgagcccag cgccggccgg gcagcgctac
     3121 tgcagccagt acgtgatgcc gcgccccggt actgctgggg gcccggatcc gacgcagcag
     3181 gcaccgtttg aggtcggcga ctacatccaa tacagcggca ccctggtcaa cggcgccaac
     3241 ggggcatacg tgtcagcgca cacgatcgag gcgaacgtcg ggatctacac gcagccgggc
     3301 acgcagccca gctacatggc gatcggcgag ttcggtgtcg gcaccgccga ccccaacgcg
     3361 accgccgcca acggcgc
//
LOCUS       DASYLX010000592         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2222509,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000592 DASYLX010000000
VERSION     DASYLX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222509"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2647
                     /gene="car"
                     /locus_tag="VGP27_14135"
     CDS             <1..2647
                     /gene="car"
                     /locus_tag="VGP27_14135"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylic acid reductase"
                     /protein_id="HEV7852471.1"
                     /translation="THPAITLNFLPMSHMMGRGGLYATLGVGGTAYFAARSDLSTFLD
                     DLALVRPTQMTFVPRVWDMIFAEVQSELDRRPDDRSAVLDEMRERLLGGRYVMAMTGS
                     APMSPEMKMFVEELLDLHLIDGYGSTEAGAVIVDGQIQRPPVIGYKLVDVPDLGYFRT
                     DRPHPRGELLVRSEGLFSGYYKRPGLTTEMFDSEGYYRTGDVFAEVGPDQLVYLDRRN
                     NVLKLSQGEFVAVSKLEALFGDSPFVRQIYVYGNSARSYLLAVVVPTDEALAGYDAAA
                     LKSALGESFADIAKEAGLQSYEIPHDFLVETSPFTLENGLLTGIRKLARPKLKEHYGD
                     RLEQLYVDLADGQADELRALRQGGGDGPVLDTVIRAAGALLGTAAADVAADAQFTDLG
                     GDSLSALTFANLLNDIFDIEVPVGVIVSPANDLAALAAYIETERQPGTKRPTFASVHG
                     RGATEAHAADLTLDKFIDAQTLAAASSLPGPSPEVRTVLLTGATGFLGRYLALEWLQR
                     MDLVGGKVICLVRAKNDDAARARLDGIFDSGDAELLRLYRRLAAEHLEVIAGDKGEAN
                     LGLDLQTWQRLADSVDLIVDPAALVNHVLPYDQLFGPNALGTAELVRIALTTRIKPFA
                     YVSTIGVGAGIEPGKFTEDGDIRVISATRKVDDSYANGYGNSKWAGEVLLREAHDLCG
                     LPVSVFRCDMILADTTYAGQLNLPDMFTRMMLSLVATGIAPASFYELDAGGNRQRAHY
                     DGLPVEFIAEAISTLGVNVIDGFETYHVMNPYDDGIGLDEFVDWLIAAGYPLQRVGDY
                     AAWLARFETAIRALPERQRQASLLPLLHNYQHPEKPIRGSMAPTDRFRTAVQSAKVGQ
                     DKDIPHVTPGVIVKYITDLELLGLL"
     gene            complement(2652..3038)
                     /locus_tag="VGP27_14140"
     CDS             complement(2652..3038)
                     /locus_tag="VGP27_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV7852472.1"
                     /translation="MLTAALIFAGLAAALHVYIFVMESLTWTSPRTRATFGTTAQEAE
                     TTKLLAFNQGFYNLFLAIVTAIGIVAICLGHTAVGGALIFTGVGSMLAAAVVLLASSP
                     DKARAAVTQGAFPLIAVGLLAVALAS"
     gene            complement(3054..4121)
                     /gene="ruvB"
                     /locus_tag="VGP27_14145"
     CDS             complement(3054..4121)
                     /gene="ruvB"
                     /locus_tag="VGP27_14145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV7852473.1"
                     /translation="MSRFDSDTEEEPEDREVSAALTVGEGDIDAGLRPRSLGEFIGQA
                     RVREQLQLVIEGAKNRGGTPDHILLSGPPGLGKTSLAMIIAGELGSALRVTSGPALER
                     AGDLAAMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVVVGKGPGATSIPLE
                     VAPFTLVGATTRSGALTGPLRDRFGFTAHMDFYEPAELERVLARSAGILGIELGSEAG
                     AEIARRSRGTPRIANRLLRRVRDYAEVRADGVITRDIAKYALEVYDVDELGLDRLDRA
                     VLSALTRSFGGGPVGVSTLAVAVGEEAATVEEVCEPFLVRAGMIARTPRGRVATASAW
                     THLGMSPPSGVPTLGQQGLFE"
     gene            complement(4118..>4570)
                     /gene="ruvA"
                     /locus_tag="VGP27_14150"
     CDS             complement(4118..>4570)
                     /gene="ruvA"
                     /locus_tag="VGP27_14150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV7852474.1"
                     /translation="VTAMIVREDSMTLYGFADADARDLFTTLLSVSGVGPKIALATLA
                     VHDAPALRQALADSDVTALTRVPGIGKRSAERMVLELRDKVGPVGAAVGLPAVNGHAV
                     RGPVVEALVGLGFALKQAEEATDKVLANEPEATQSTALRSALSMLGNK"
BASE COUNT          775 a         1544 c         1489 g          762 t
ORIGIN      
        1 cacccatccc gcgatcaccc tcaacttcct gccgatgagc cacatgatgg gccgtggtgg
       61 actgtacgca acgctcggag tcggcggcac ggcgtatttc gctgcccgca gcgatctttc
      121 gacgttcctc gacgacctcg ctctggtgcg gccgactcag atgaccttcg tgccacgggt
      181 gtgggacatg atcttcgctg aggtccaaag cgagctggac cgcaggcccg acgacaggtc
      241 cgcggtgctg gacgagatgc gcgagcgcct gctcggcggt cgctacgtca tggcgatgac
      301 gggttcggcc ccgatgtcac ccgagatgaa gatgttcgtc gaggaactgc tcgacctgca
      361 cctgatcgac ggctacggct ccaccgaagc aggcgccgtg atcgtcgacg gacagattca
      421 gcgcccaccg gtgatcggct acaagctggt cgacgtgccg gacctcggct acttccgcac
      481 cgaccggccg cacccgcgtg gcgagctgtt ggtgaggtcc gagggcttgt tctccggcta
      541 ctacaagcgc cccgggctga cgacagagat gttcgacagc gagggctact accgcaccgg
      601 cgacgtgttc gccgaggtcg gtccggacca gctggtgtat ctggaccgcc gcaacaacgt
      661 cctcaagctg tcgcagggcg aattcgtcgc tgtctccaag ctcgaggccc tgttcggtga
      721 cagcccgttt gtgcgacaga tctacgtcta tggcaacagc gcccggtcct acctcctcgc
      781 ggtggtcgtg ccgaccgacg aggcgcttgc cggctacgac gcggcagcgc tcaaatccgc
      841 tctcggcgag tcctttgccg acatcgcgaa ggaagcggga ctgcagtcgt atgagattcc
      901 gcacgacttt cttgtcgaga cttctccgtt cacgctggag aacggcttgt tgaccggaat
      961 ccgcaagctg gcgcggccga agctcaagga gcactacggc gaccgcttgg agcagcttta
     1021 tgtcgatctg gccgacgggc aggccgacga gttgcgcgca ctccggcagg gcggcggcga
     1081 cggcccggtg ctggataccg tcatccgcgc ggcgggtgcg ctgctcggta cggcggctgc
     1141 agatgtcgcg gccgacgctc agttcaccga tctcggcggt gattcattgt cggcgttgac
     1201 gttcgcgaac ctgctcaacg acatcttcga catcgaggtt ccggtcggcg tcatcgtgag
     1261 cccggccaac gacctggccg cgcttgcggc ttatatcgag accgagcggc agcccggcac
     1321 caagcgtccg acgttcgcgt ccgttcacgg ccgcggcgcg accgaggcgc acgccgctga
     1381 tctgacgctg gacaagttca tcgacgcgca gacgctcgcc gcggcgtcgt cgctgccggg
     1441 cccgagcccc gaggtgcgca cggtgttgct caccggcgca accggatttc tcggccgcta
     1501 cctggcactg gaatggttgc agcgaatgga tctcgttggc ggcaaggtca tctgcctcgt
     1561 tcgcgccaag aacgacgatg ccgcacgggc acgcctagac ggcatcttcg acagcggtga
     1621 tgcagaactg ctgcgcctct accgccggtt ggccgccgag cacctcgagg tgatcgccgg
     1681 tgacaagggc gaggccaacc tcgggctcga tctgcagacg tggcagcggc tcgccgattc
     1741 cgtcgacctc atcgtggatc cggcggcact ggtcaaccac gtccttccgt acgaccagct
     1801 gttcggaccc aatgcgctcg gcacggccga actcgtccgg atcgcgctga ccaccaggat
     1861 caagccgttc gcgtacgtgt cgaccatcgg cgtcggcgcg ggcatcgaac cgggcaagtt
     1921 caccgaggac ggagacatcc gggtcatcag cgccacccgc aaggtcgacg acagctacgc
     1981 caacggctat ggcaacagca agtgggccgg tgaggtgctg ctgcgcgagg cgcatgacct
     2041 gtgcggcctg ccggtttcgg tgttccgctg cgacatgatc ctcgcggaca ccacctacgc
     2101 cgggcagctc aacctgccgg acatgttcac ccgcatgatg ctgagcctgg tcgccaccgg
     2161 tatcgcaccc gcgtcgttct atgaactcga cgcaggcggg aaccggcagc gggcgcacta
     2221 tgacggtctg ccagtggaat tcatcgccga ggcgatttcc acgttgggtg taaatgtgat
     2281 cgacggattc gagacttacc acgtgatgaa tccgtacgac gacggcatcg gcctcgacga
     2341 gttcgtcgac tggctgatcg ccgcggggta tccgcttcag cgcgtgggcg actacgccgc
     2401 ctggctggcg cgtttcgaaa cggcgatccg ggcactgccg gaacggcagc gacaggcctc
     2461 gctgctaccg ctgctgcaca actaccagca tccggagaag ccgatccgcg gatcgatggc
     2521 gccgaccgac cggttccgta ctgcagtgca gagtgcgaaa gtcgggcagg acaaggacat
     2581 tccgcacgta acgcccggtg tcatcgtcaa atacatcacc gatctggaat tgctcggcct
     2641 gctctgaccc gttaggacgc aagcgccacc gcaaggagcc cgaccgcgat gagcgggaag
     2701 gctccttgcg tgactgcggc acgcgccttg tcgggcgagg acgccagcag caccaccgcc
     2761 gctgccagca tcgacccgac tccggtgaag atcagtgcgc cgccaaccgc ggtgtgtccc
     2821 aggcatattg cgacgatgcc gatcgccgtc acgatagcca ggaacaagtt gtagaatccc
     2881 tggttgaatg cgagcagctt ggtggtctcg gcttcttgcg ccgtggtgcc gaaggtcgca
     2941 cgggtgcgcg gcgacgtcca cgtcagcgac tccatcacga agatgtacac atgcagcgcc
     3001 gcggccagcc cagcgaaaat caaagcggct gtcagcattc ggctctcctc tggctactcg
     3061 aacaacccct gttgacccag cgtcggcacg ccgctcggcg ggctcatccc caggtgcgtc
     3121 cacgccgatg ccgtcgcgac gcgaccgcgg ggggtgcgcg cgatcatccc ggcccgcacc
     3181 aggaacggct cgcacacctc ctcgacggtg gccgcttcct caccgaccgc gaccgccagc
     3241 gtcgaaacgc cgaccgggcc tccgccgaaa cttcgggtca gcgccgacag cacggcgcgg
     3301 tccagccggt cgagtccgag ctcgtcgacg tcgtacacct cgagcgcgta tttcgcgatg
     3361 tcgcgggtga tgacgccgtc ggcacgaacc tcggcgtagt cgcgcacccg gcgcagcagc
     3421 cggttcgcga ttcgcggtgt accacgtgag cggcgggcga tctcggcgcc ggcttcggag
     3481 ccgagttcga taccgaggat gcctgccgag cgggcgagca cccgctcaag ttcggcgggc
     3541 tcgtagaaat ccatatgggc ggtgaaaccg aaacgatcac gcagcgggcc ggtcagcgcg
     3601 cccgaccggg tggtagcgcc gacgagtgtg aacggcgcga cctccagcgg aatcgacgtc
     3661 gcgccgggac ctttgccgac gacgacgtca acgcggaagt cctccatcgc gaggtagagc
     3721 atctcctcgg cgggccgcgc gatgcggtgg atctcgtcga tgaacaacac gtcgtgctcg
     3781 acgaggttgg acagcatcgc agccaggtcg cctgcgcgct cgagtgcggg accggaagtc
     3841 acccgcaacg ccgagcccaa ttcgccggcg atgatcatcg ccaatgacgt cttgcccagc
     3901 cccggcgggc cggacagcag gatgtgatcg ggtgtgccac cgcggttctt ggcgccttcg
     3961 atgaccagct gcagctgttc gcggacccgg gcctggccga tgaactcgcc cagcgaccgc
     4021 ggtcgcaggc cggcgtcgat gtcgccttcg ccgacggtca gtgccgcaga gacctcgcgg
     4081 tcctccggtt cctcttcggt gtcgctgtcg aagcggctca tttgttgccg agcatcgaca
     4141 gcgccgaacg caacgccgtc gactgcgtcg cctccggctc attggccagc accttgtcgg
     4201 tggcctcctc ggcctgcttg agcgcaaagc caaggccgac aagtgcttcc acgacggggc
     4261 cgcgtacggc gtggccgttg acggcgggca acccgacagc ggcccccacc ggaccgacct
     4321 tgtcacgcag ttcgagcacc atccgttcgg cgctgcgctt gccgatcccc ggcaccctgg
     4381 tcagcgcggt cacgtcgctg tcggcgagtg cctgcctcag ggcgggcgca tcgtgcacgg
     4441 ccagcgtggc cagtgcgatc ttcggtccca cgccggacac cgacagcaga gtggtgaaca
     4501 ggtcgcgcgc gtcggcgtcg gcgaagccgt agagcgtcat cgagtcctcg cgcacgatca
     4561 tcgcggtcac
//
LOCUS       DASYLX010000593         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_578340,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000593 DASYLX010000000
VERSION     DASYLX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578340"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VGP27_14155"
     CDS             complement(<1..1278)
                     /locus_tag="VGP27_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV7852475.1"
                     /translation="MNHSSDVATARLVSVNVGIPADVTWRGNTVHTGVYKSPVAGPVM
                     VRRLNIDGDGQGDLGGHGGEQRAVMVYQTESYDYWSEFLARDDLEPGNFGENFTITGL
                     ADDQVCIGDRYAIGEAEFEVTQPRVTCFRVGMRLGEPDMPNLLVAHHRPGFYFRVITE
                     GHVQAGDPIVRTRRGRHELTVADIDALLYLPRRDSGLLRKAVDVPALSPGWQQSFTDL
                     LTADQTSTTAEQPVVGAEPGWSGFRRLRVTGTHRESADIMSIRFEAVDGAPLPIPRPG
                     QYLTLRFPDAGDPAPLRTYSLSSYGPKYRISVKREEHGAVSRWLHNNIRAGSVVDVAA
                     PRGDFYLMEAAGPIALLSAGVGVTPVLAMLHALADTGSGREIWWLYTTRDADTYAFGR
                     EVSDLIAELPHARQQVVYTSSAGRLDSRAIAAVG"
     gene            complement(1275..1598)
                     /locus_tag="VGP27_14160"
     CDS             complement(1275..1598)
                     /locus_tag="VGP27_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852476.1"
                     /translation="MPTGCGLRLADLDREKLQVSLDTKVIGPTMLAKHFAPLLNEGGS
                     IVLFSGVNASKVNVGYLGGAITDGAVDFLTRWPAVELAPIRVIAVSPGVIDTGARDSP
                     EGGRW"
     gene            complement(1608..2129)
                     /locus_tag="VGP27_14165"
     CDS             complement(1608..2129)
                     /locus_tag="VGP27_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV7852477.1"
                     /translation="MDLVAVGPSDEEFLLELLNTTPVVDGEPRDDLAQPKVARAWMRA
                     HGVASTNAELTALLDARSVLQAVVRGEQAPPALQRFIDSVALKPTASRDGVDWRLTVK
                     GGPGGAVRAVLAWDGLRTSSPGRLRPCANAECRLFLIDRSKPNTARWCSMAICGNRMK
                     ARRHYRRARAGEG"
     gene            2215..3087
                     /locus_tag="VGP27_14170"
     CDS             2215..3087
                     /locus_tag="VGP27_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7852478.1"
                     /translation="MTARVHHRYATVDGRRVFYREAGDIGAPAIVLLHGFPTSSFMFR
                     DLIPRLADRYHMIAPDHLGFGLSAAPSAAEFDYTFDALTDLTQGLLDQLGLTRYAIYV
                     QDYGAPIGWRLALRHPQAITAIVTQNGNGYDEGFVESFWTTVWNYHREQNPDTEAAIR
                     TALDEESIKWQYLTGVADETLVSPDTWTRDAALLARPGNDEIQLALFRDYATNAPMYP
                     ALHDYLRSSGVPVLAVWGQGDPIFGPDGARAFGKDVQDAEIHLLDGGHFLLETAAEEV
                     AGLMRDFLHRATAA"
     gene            3116..3994
                     /locus_tag="VGP27_14175"
     CDS             3116..3994
                     /locus_tag="VGP27_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV7852479.1"
                     /translation="MGLAWQQGPLAAGSVGHFLAAEPLPERLLFVEPLRRRMRARQGA
                     EWIADSEDVLLLHEPGRYPVAYFPIDDICDGVLVAEDRVTQHGELGDMQWFTIVADGR
                     EIPHAAWQHTALPSYATDLTRRVAFGWQAVDAFYEEDERIAGHAADPYHRVDIRATSR
                     HLAIRDGDRVIADSHRPVVLYESGFAPRWYVTRDDIDQGALEPVEGQTFCPYKGLADY
                     YTIGAHKKAAWSYVNAWPEAGRVSNLVSFEPDKIDLWLDDERLHLEPGQTVVAHGIDR
                     GLDADELVRDAARSAR"
     gene            3991..4425
                     /locus_tag="VGP27_14180"
     CDS             3991..4425
                     /locus_tag="VGP27_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7852480.1"
                     /translation="MIASLEVVVLPVADPDKSLDFYRNRLGFALDVDHWPTKNFRVVR
                     LTPETSRTSIQFGLGLTDAAPGSVQGLYLVVSDITAVRHELVDRGVGVSSIRHKDVDG
                     GQWRGRFRLGANPDRADYSSFADFTDPGGNAWIIQERGHRDA"
     gene            4418..>4717
                     /locus_tag="VGP27_14185"
     CDS             4418..>4717
                     /locus_tag="VGP27_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV7852481.1"
                     /translation="MHSRPSVLVFDVNETLIDIESLEPHFVRIFGDGRVLREWFGQLV
                     MYSMAVTLSDTYVDFFSLGQAVLRMTADVHHVRIGDDDLAALNAGMQTMPAHRDVA"
BASE COUNT          842 a         1571 c         1484 g          820 t
ORIGIN      
        1 ccccactgcc gcgatggctc ggctgtccag ccggccggcc gagctggtgt agacgacctg
       61 ctgtcgcgcg tgcggcaact cggcgatgag gtcgctgact tcgcgcccga acgcatacgt
      121 atccgcatct cgggtggtgt acagccacca gatctcacgc ccgctgccgg tgtcggccag
      181 cgcgtgcagc atcgcgagca ccggcgtgac cccgactccc gccgacagca gagcgatcgg
      241 gcctgccgct tccatcagat agaagtcacc gcgtggcgcg gcgacatcga cgacggaacc
      301 ggcgcgaatg ttgttgtgca gccagcgact gacggccccg tgctcctcgc gcttgacgct
      361 gattctgtac ttggggccgt aactcgacag cgagtaggtg cgcagcggag ccgggtcgcc
      421 tgcgtccggg aaccgcagag tcagatactg gccgggccgt gggatgggca acggcgcgcc
      481 gtccacggcc tcgaaccgta tcgacatgat atcggcgctc tcgcgatgag tgcccgtgac
      541 acgaagtcgc cggaaaccgc tccagcccgg ctccgcgccg actaccggtt gttctgcggt
      601 ggttgaggtt tggtctgcgg tcagcaggtc ggtgaaggat tgttgccaac cggggctgag
      661 cgcgggcacg tcgacggcct tacggagcag tccgctatcc cgacggggca gatacagaag
      721 cgcatcgata tccgcgacgg tgagttcgtg cctgccgcgt cgcgtgcgca cgatcgggtc
      781 gccggcctga acgtgtccct cggtgatgac gcgaaaatag aagcccggac ggtgatgggc
      841 gacaaggaga ttgggcatat ccggttcgcc gaggcgcatg ccgacccgga agcaggtcac
      901 ccgcggctgg gtcacctcga actccgcctc gccgatggcg tagcggtcgc cgatgcagac
      961 ctggtcatcg gccagtccgg tgatcgtgaa gttctcgccg aaattgcccg gttccagatc
     1021 gtctcgtgcc aggaattcgc tccagtagtc gtaggactcc gtttgataca ccatcacggc
     1081 tcgttgctcg ccgccgtgac cgccgaggtc accttgaccg tcgccgtcga tgttcagcct
     1141 gcgcaccatc accggtccgg ccaccggact cttgtagacg ccggtgtgca cggtgttccc
     1201 gcgccacgtc acatcggccg ggatcccgac attgacggaa accaacctgg ctgtcgcgac
     1261 gtcagatgag tggttcacca ccgtccacct tcaggtgagt cccgtgcgcc ggtgtcgatg
     1321 acgcccggtg agacggcgat gacgcggatc ggcgccagct cgaccgccgg ccaccgggtg
     1381 aggaaatcaa cagcaccatc ggtgatagcg ccgccgaggt atccgacgtt gaccttggag
     1441 gcgttcacgc cggagaacag cacgatcgat cctccctcat tcagcagcgg agcgaaatgc
     1501 tttgccaaca tcgtcggccc gatcaccttg gtatcgagcg acacttgcaa tttctcgcgg
     1561 tccaggtcgg cgagtcgtaa cccgcagcct gtgggcaggc cggttggtca gccctcgcca
     1621 gcgcgcgcac ggcggtagtg ccggcgggct ttcatccgat tgccacagat cgccatcgag
     1681 caccagcgcg cggtattggg cttgctccgg tcgatcagaa acaatcggca ttcggcgttc
     1741 gcgcacggcc gaagccgccc cggactggag gtccgcaggc catcccaggc cagcaccgcg
     1801 cgcaccgctc cgcccggccc tcctttcacg gtcagccgcc agtccacacc gtcacgggac
     1861 gcggtcggct tcaaggcgac ggagtcaatg aaccgctgca gggccggcgg cgcctgctca
     1921 cccctcacca ccgcctggag taccgaccgc gcgtcgagga gtgccgtcaa ctcggcattg
     1981 gtggacgcga ccccgtgcgc gcgcatccat gcacgggcca ccttcggctg cgcgagatcg
     2041 tcgcgcggct ccccgtccac caccggtgtg gtgttgagca gctcgagtag gaactcctcg
     2101 tcggaggggc cgactgcaac gagatccata tctaacctcc aaattgatat tgacaggtta
     2161 caggataagg cgttgactgt cgctaaccat caaacattgt taaggaggtt agtaatgacg
     2221 gctcgagttc accaccgtta cgccaccgtc gacggccggc gcgtcttcta ccgcgaggcc
     2281 ggagacatcg gagcacccgc gatcgtcctg ctgcacggat ttccgaccag ctcgttcatg
     2341 ttccgcgatc tgatcccgcg gctcgccgac cgctaccaca tgatcgcacc cgatcatctc
     2401 ggcttcggat tgtcggccgc cccgtcagcg gcggagttcg actacacctt cgatgcgctg
     2461 accgacctca cacagggcct gctcgatcaa ctcggtctga cccggtacgc gatctacgtc
     2521 caggactatg gtgccccgat cggatggcgg ctggcgctgc gacacccgca ggccattacg
     2581 gccatcgtca cgcagaacgg caacggctac gacgagggtt ttgtcgaaag cttctggacg
     2641 acggtgtgga actaccaccg cgagcagaat ccggacaccg aggccgccat tcgcaccgcc
     2701 cttgacgaag agtccatcaa gtggcagtac ctcaccgggg ttgccgacga gacactggtc
     2761 agcccggaca cctggacacg tgacgccgcc ctgctggcgc ggccgggtaa cgacgagatc
     2821 cagctcgccc tcttccgcga ctacgccacc aacgcgccca tgtatcccgc gctgcacgac
     2881 tatctgcgca gcagcggggt gccggtgctg gccgtgtggg gccagggcga cccgatcttc
     2941 ggccccgacg gtgcccgtgc gttcggcaag gacgtccaag acgccgagat ccacctgctc
     3001 gacggaggcc acttcctgct ggagaccgca gccgaagagg tcgcagggtt gatgcgggac
     3061 ttcctgcacc gagccaccgc ggcttgaaca gcatttccaa agagaggaaa cgacaatggg
     3121 attggcatgg cagcagggcc ccttggcggc cgggtccgta ggccactttc tggccgcaga
     3181 accgctaccc gaacggctgc ttttcgtcga accgctgcgc aggcgcatgc gggcacggca
     3241 gggcgccgag tggatcgccg acagcgagga cgtgctgctg ctccacgaac cgggccgcta
     3301 ccccgtcgcg tacttcccca tcgacgacat ctgtgacggt gtcctcgtcg ccgaggaccg
     3361 agtcactcaa cacggtgaac tcggcgatat gcagtggttc acgatcgtcg cggatggacg
     3421 agagatcccc cacgccgcgt ggcagcacac cgcgctgccg tcatatgcga cggacctgac
     3481 tcgacgggtg gcattcggct ggcaggcggt ggacgccttc tacgaagagg acgagcgcat
     3541 tgccggtcac gccgcagatc cctatcaccg cgtcgatatc cgtgccacat cgcgccatct
     3601 cgccattcgt gacggcgacc gtgtgatcgc cgattcgcat cggcccgtgg tgctctacga
     3661 gtcgggcttc gcgccgcggt ggtatgtgac cagggacgac atcgaccagg gtgcgttgga
     3721 acccgttgaa ggccagacgt tctgcccgta caagggcttg gccgactatt acaccatcgg
     3781 tgcgcacaag aaggccgcgt ggtcgtatgt caacgcatgg ccggaagccg gcagggtgag
     3841 caacttggtg tcattcgagc cggacaagat cgacctgtgg ctcgacgacg aacgcctcca
     3901 cctcgaaccc ggacagaccg tggtcgcgca cggcatcgac cgcggactcg acgccgacga
     3961 actggtccgt gacgccgcaa ggagcgcacg atgatcgctt cgctcgaagt cgtcgttctg
     4021 cccgtcgcgg atcccgacaa gtcgttggac ttctaccgca acaggctagg tttcgctctc
     4081 gacgtcgacc actggccaac gaagaacttc cgcgtcgtcc ggctcacacc cgaaacgtcg
     4141 cgcacatcaa tccaattcgg gctagggctg accgatgcgg cgcccggctc ggtccaaggg
     4201 ctgtacctcg tcgtgagcga tatcacggcg gttcggcacg agctcgtcga tcgaggtgtc
     4261 ggtgtcagtt cgatcagaca caaggacgtc gacggcgggc agtggcgggg gcgtttccgc
     4321 ctcggcgcca accctgatcg ggccgactac tcgagcttcg cagacttcac agatcccggc
     4381 ggcaatgcct ggataattca ggagcgtggc caccgcgatg catagccgtc cgtcggttct
     4441 ggttttcgat gtcaacgaga ccctcatcga catcgagtcc ctggagccgc acttcgtgag
     4501 gatcttcggc gacggccggg tacttcgcga gtggttcggc caactggtca tgtattcgat
     4561 ggccgtcacg ctgtccgata cctacgtcga cttcttctct ctcggccaag cagtgttgag
     4621 gatgacggcc gacgtccacc acgtccggat cggcgacgat gacctcgccg ctttgaacgc
     4681 agggatgcag accatgcccg cacaccgcga tgtcgcg
//
LOCUS       DASYLX010000594         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2771138,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000594 DASYLX010000000
VERSION     DASYLX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771138"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VGP27_14190"
     CDS             <1..751
                     /locus_tag="VGP27_14190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212102.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV7852482.1"
                     /translation="AVGYQPTLADEMGELQERITSTRGHSITSMQAVYVPADDYTDPA
                     PATTFAHLDATTELSRNVFSKGIFPAVDPLASSSTILDPSVVGEEHYRVAQEVIRILQ
                     RYKDLQDIIAILGIDELAEEDKQLVQRARRIERFLSQNMMAAEQFTGQPGSTVPLKET
                     IEAFDRLTKGDFDHLPEQAFFLIGGLEDLRRKAESLGAKMDDSGSDGAPVSGDKSDED
                     SDDDSAKDDDSAKDDDTDDDAAKDDGEDKSD"
     gene            754..1119
                     /locus_tag="VGP27_14195"
     CDS             754..1119
                     /locus_tag="VGP27_14195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV7852483.1"
                     /translation="MAELDVDIVAVEREIWSGKATFVFTRTTAGEIGILPRHIPLVAQ
                     LVDDAMVRVEREGEDDLRIAVDGGFMSVTDEGVIILAESAELESEINADAAKRDSESD
                     DEATAAKGRARLRALGQID"
     gene            1128..1574
                     /locus_tag="VGP27_14200"
     CDS             1128..1574
                     /locus_tag="VGP27_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HEV7852484.1"
                     /translation="MSASMLIMAALVGVLLLVVIALSYRLWKLRQVGGTAAILRDYPA
                     VGGQGWRHGVMRYRGGEAGFYRLSSLRWWPDRRLSRRGLEIVSRRSPRGDEFDIMTAE
                     IVILELRDTSPERKTGYEVALDRGALTAFQSWLESRPSPRARRRTA"
     gene            complement(1571..2149)
                     /locus_tag="VGP27_14205"
     CDS             complement(1571..2149)
                     /locus_tag="VGP27_14205"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV7852485.1"
                     /translation="MAVHLTRIYTRTGDDGTTGLSDFSRVSKNDARLAAYADCDEANA
                     AIGVAVSLGHPDEQMLKVLRQIQNDLFDAGADLSTPVVEDPEHPPLRISQSYIDRLEA
                     WCDQFNAPLPALDSFVLPGGTPLSALLHVARTVARRAERSAWLAVEAHPDSVSVLPAK
                     YLNRLSDLLFILGRVANPGGDVLWQPGGFASD"
     gene            2220..>2512
                     /locus_tag="VGP27_14210"
     CDS             2220..>2512
                     /locus_tag="VGP27_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV7852486.1"
                     /translation="MSERFVVTGGDRLSGEVAVGGAKNSVLKLMAATLLAEGTSTITN
                     CPDILDVPLMAEVLRGLGANVELEGGTVRITSPDEPKYDADFAAVRQFRASVCV"
BASE COUNT          478 a          787 c          837 g          410 t
ORIGIN      
        1 cgcggtggga taccagccga cactggccga cgagatgggt gagctccaag agcgcatcac
       61 gtcgacccgc ggccactcga tcacctcgat gcaggccgtg tacgtgcccg ccgacgacta
      121 cactgacccg gcgcccgcca caaccttcgc ccatttggac gccaccaccg agctcagccg
      181 aaatgtgttc tccaagggca tctttccggc ggtcgatccg ctggcgtcca gctcgacgat
      241 cctcgatccg agcgtggtcg gcgaggagca ctaccgtgtg gcccaggaag tcatccgaat
      301 cttgcagcga tacaaggacc tccaggacat catcgcgatt ctgggcatcg acgaactggc
      361 cgaagaagac aagcagttgg ttcagcgcgc ccgccgcatc gagcgcttcc tgtcccagaa
      421 catgatggcc gccgaacagt tcaccggcca gcccggctcg accgtgcctc tgaaggagac
      481 catcgaggcg ttcgacaggc tcacgaaggg cgatttcgac caccttcccg agcaggcgtt
      541 cttcctgatc ggcggtctcg aagatctgcg gcgcaaggcc gagagcctcg gcgccaagat
      601 ggacgacagc ggtagcgacg gcgcaccggt gtccggggac aagagcgacg aggactcgga
      661 cgacgattcc gcgaaggacg acgattccgc gaaggacgac gacacggacg acgacgccgc
      721 gaaggacgac ggcgaagaca agagtgacta gtcatggctg aactggatgt ggacatcgtc
      781 gcggtagaac gcgagatctg gtcggggaag gccactttcg tgttcacccg caccactgcg
      841 ggtgagatcg gtatcttgcc gcgtcacatc ccgctggtgg cgcagctggt tgacgacgcc
      901 atggtcaggg tcgaacgcga aggtgaggat gatctgcgca tcgccgtcga cggcggcttc
      961 atgtccgtca ccgacgaggg cgtgatcatc ctcgccgagt ccgctgaact cgagtccgag
     1021 atcaacgccg atgcggcgaa gcgggactcc gagtccgacg acgaggcgac cgcagccaaa
     1081 ggccgcgcca ggctgcgcgc tctcggccag atcgactagg gccaccgatg agcgcgtcca
     1141 tgctgatcat ggccgcgctg gtgggtgtgc ttcttctcgt cgtcatcgcc ctgagctacc
     1201 ggctgtggaa gctgcgtcag gtcgggggta ccgcggcgat tctgcgtgac tatccggcag
     1261 tcggaggcca gggctggcgg cacggggtaa tgcgttatcg cggcggcgaa gccggcttct
     1321 accggctctc cagcctgcgg tggtggcctg atcgtcggct cagccgccga gggctggaga
     1381 tcgtgtcgcg ccggtcgcca cgaggcgatg agttcgacat catgacggcc gagatcgtga
     1441 tcctcgaact gcgcgacacg agcccggaac gcaagaccgg ctacgaggtg gcgctggatc
     1501 gcggtgcgct gaccgccttt caatcgtggc tggagtcgcg accgtcgcct cgagcgcggc
     1561 gcaggacggc ctaatcgctt gcaaaaccgc ccggttgcca cagcacatcg ccgccgggat
     1621 tggcgactct gccgaggatg aagagcagat cggaaagcct gttgaggtat ttggccggta
     1681 gcacactgac gctgtcgggg tgagcctcga ccgccagcca ggccgaccgc tcggcacgtc
     1741 gcgcgacggt tcgggccaca tggagcaagg cggacaacgg agtaccgccc ggcagcacaa
     1801 aagaatccag tgcaggcagg ggtgcgttga attggtcgca ccacgcctcc aaccggtcga
     1861 tgtagctctg gctgatacgc agtggtgggt gctcgggatc ctcgacgacc ggagtggaca
     1921 ggtcagcgcc cgcgtcgaag aggtcattct gaatctgccg cagcaccttg agcatttgct
     1981 cgtcggggtg acccaacgaa acggcaacgc cgatggcggc gttggcctca tcgcagtcgg
     2041 cgtacgccgc gagccgcgca tcgttcttcg aaaccctgct gaagtcgctc aacccggtcg
     2101 ttccgtcgtc accggtacgg gtgtaaatgc gggtcaggtg cacagccatg gtcaaacggt
     2161 accgccggcg cctcaccgag gattcggaca ccgcccgcac cgctgtttaa actaacgcgg
     2221 tgagcgagcg attcgtggtc accggtggag accggttgtc aggcgaagtt gccgtcgggg
     2281 gagccaagaa cagcgtgctg aagctgatgg ctgccacgct gctggccgaa ggcaccagca
     2341 cgatcacgaa ttgcccggac attctcgacg tgccgctgat ggcggaggtg cttcgcgggc
     2401 tcggagccaa cgtcgaacta gagggcggca cggttcgcat cacatcaccc gacgaaccca
     2461 agtacgacgc ggatttcgct gccgtccgcc agttccgggc ctcggtgtgc gt
//
LOCUS       DASYLX010000595         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_907271,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000595 DASYLX010000000
VERSION     DASYLX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907271"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VGP27_14215"
     CDS             <1..671
                     /locus_tag="VGP27_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7852487.1"
                     /translation="AATDDALRVIHIRGEGDDFCAGADWVATNSGGARPRTGDLVRRI
                     PHTSHRLIELVHSIHLPVVCTVRGWAVGLGCNLALAADFTVAADDAVFWAPFVTRGFS
                     PDSGATWLLPRLVGMARAKRMLLLGEKVSASDAAEWGLIHDAVDPAGLDDAAGRLLVA
                     LASAPTVAVGLAKQAIHYGQHATLPQTMNQELYNLELSCRTTDFKEGLAAFRERRTPE
                     FHGR"
     gene            702..1514
                     /locus_tag="VGP27_14220"
     CDS             702..1514
                     /locus_tag="VGP27_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7852488.1"
                     /translation="MAENAYDTIKYERDGHKATVTLNRPDALNALSPYMIGELRTAYD
                     EAENDDDVWLLIVTGAGRAFCTGADAKQIPQDGKVINERAYLSTYDQWEAPQEGTPPF
                     RRMAKPVLAAINGICCGAGLDWVTTGDIVIASDKATFFDPHVSIGLVAGREMVRLAKV
                     LPRNVALRMALTGKHERMDAQRAYELGMVSEVVTHDRLLERAHEIADIVNSNAPLAVR
                     GTRLAIHRTLDLPLHDGEILAEAFRERNLHTEDSLEGPRAFVEKRAPKWQCR"
     gene            1511..>1990
                     /locus_tag="VGP27_14225"
     CDS             1511..>1990
                     /locus_tag="VGP27_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7852489.1"
                     /translation="MSEGFDTIRLDVDAGDHVATITLNRPDALNAFNRTMCEEMARAW
                     RSVKLDDSVNAVVLRAAGDRAFSAGLDVKSSYGQPDNVWNHEDPGEPLSPKWQKMWKP
                     VVCAVQGICTAGAFYFVNESDVVICSQDATFFDSHVSAGLVCALEPIGLMRRVGLGET
                     "
BASE COUNT          351 a          669 c          664 g          306 t
ORIGIN      
        1 cggccgccac cgacgatgcg ttgcgggtca ttcacattcg cggtgaaggc gacgacttct
       61 gcgcgggcgc cgactgggtg gccaccaatt ccggcggcgc acggccgcgc acaggcgatc
      121 tggtccggcg catcccccat acctcccacc ggctgatcga actcgtccac agcatccatc
      181 tgcccgtcgt ctgcaccgtg cggggctggg ccgtcggcct cggttgcaat ctggcgctgg
      241 ccgccgactt caccgtggcc gccgacgacg cggtgttctg ggcaccgttc gtgacccgag
      301 gcttcagccc cgactccggc gcgacgtggc tgctgccacg gctggtcggc atggcacgcg
      361 ccaagcgaat gctgttgctc ggcgagaagg tgtcggcatc tgacgccgcg gagtgggggc
      421 tcatccacga cgccgtcgac ccggccggac tcgacgacgc cgccggccgg ctgctggttg
      481 cgcttgcatc cgcaccgacg gtggcggtcg ggctggccaa gcaggccatt cactacggcc
      541 agcacgccac cctgccgcag acgatgaacc aggaactgta caacctggag ctctcttgtc
      601 ggacaacgga tttcaaagag ggtctggccg cgttccgcga acgacgcacg ccggagtttc
      661 acggccgatg accgaccagc gactcgacga agggaacacc gatggccgag aatgcttacg
      721 acaccatcaa atacgaacgc gacgggcata aggccaccgt cacactgaac cggccggacg
      781 ccctcaacgc gctgagcccc tacatgatcg gcgagttgcg caccgcctac gacgaggcgg
      841 agaacgacga cgatgtctgg ctcctcatcg tcaccggtgc cggccgggcg ttctgcacag
      901 gtgccgacgc gaagcagatt ccccaggacg gcaaggtcat caacgaacgg gcctacctgt
      961 cgacgtatga ccagtgggag gcgccccagg agggcacccc gccgtttcgc cggatggcca
     1021 agcctgtgct ggcggccatc aacggcatct gctgcggcgc gggcctggac tgggtcacca
     1081 ccggcgacat cgtgatcgcc tccgataagg ccaccttctt cgatccacac gtcagcatcg
     1141 gcctggtggc aggacgcgag atggtgcggc tggcgaaggt gctgccccgc aatgtcgcgc
     1201 tgcgcatggc gctgacggga aagcacgaac ggatggacgc gcagcgggca tacgaactcg
     1261 gaatggtcag cgaggtcgtc acccacgatc gcctgctcga gcgcgcgcat gagatcgccg
     1321 acatcgtgaa cagcaacgcg ccgctggcgg ttcgcggcac gcggctggcc atccacagaa
     1381 cgctggacct gccgctgcac gacggcgaga ttctcgccga ggcgttcagg gagcgcaatc
     1441 tgcacaccga ggactcgctc gaggggccga gggcgttcgt ggaaaagcgc gcacccaagt
     1501 ggcagtgccg atgagcgaag gtttcgacac catccggctc gacgtcgatg ccggcgacca
     1561 cgtcgccacc atcacgttga accggcccga tgcgctcaac gcgttcaacc gcacgatgtg
     1621 cgaggagatg gcgcgcgcgt ggcgctccgt gaagctcgac gattcggtga acgccgtcgt
     1681 cctgcgtgcg gcgggcgacc gcgcgttcag cgccgggctc gacgtcaagt cgtcctacgg
     1741 ccagcccgac aacgtgtgga atcacgagga tcccggcgag ccgctgagcc cgaagtggca
     1801 gaagatgtgg aagccggtcg tctgcgcggt gcagggcatc tgcactgcgg gcgcgttcta
     1861 cttcgtcaac gaatccgacg tcgtgatctg ttcgcaggac gccacgttct tcgactcaca
     1921 cgtgtccgcc gggctggtgt gtgcgctcga accgatcggc ctgatgcggc gcgtgggcct
     1981 tggtgagacc
//
LOCUS       DASYLX010000596        11259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_797753,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000596 DASYLX010000000
VERSION     DASYLX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797753"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..434)
                     /locus_tag="VGP27_14230"
     CDS             complement(6..434)
                     /locus_tag="VGP27_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852490.1"
                     /translation="MAESKLSAEFDKISDKAKAASDALSAANERTREQLANDVDDARD
                     RATAAADHVKGKADDARDAASSHWQELRDKWHAHVDEVHSRVKKHNDKIDADGAAMDA
                     DLSESYAYDAIDFALDAIEEAEYAVLDAMYARANAAALSV"
     gene            738..1343
                     /locus_tag="VGP27_14235"
     CDS             738..1343
                     /locus_tag="VGP27_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV7852491.1"
                     /translation="MANANVRPLLGQQLWKWTLAAGVLAIALGGIVLAWPGPTILVAS
                     TLFGVYLLLTGLIGLSMAFTLPRSPGMRVLLFISGALAVVLAILSFRHFGDAYAVLLL
                     SLWIGIGFVFQGISATAAAIGESNLPGRGWYVVLGVISVIAGLVVLVWPFDSIVVLVL
                     VAGVWLVVLGVVQIIQSFQIRKDARTVREAIDSVSERFEAR"
     gene            1349..1891
                     /locus_tag="VGP27_14240"
     CDS             1349..1891
                     /locus_tag="VGP27_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7852492.1"
                     /translation="MDSELNARFECEVVPLRASLYRHACRITRNDADADDLVQETMMR
                     AYVGFKSFRSDSNLKAWLFRILTNTYINGYRKERRQPVQCSMEQVTEQYLAAAYARCV
                     PGQRSVEDYVLDSLPDKDIEMAMQTLPSQFREVVYYADVEGYRYKEIAAMMKTPRGTV
                     MSRLHRGRERLRAVLTPAGG"
     gene            1947..3068
                     /locus_tag="VGP27_14245"
     CDS             1947..3068
                     /locus_tag="VGP27_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7852493.1"
                     /translation="MAWADWHSLVGKGRLLWRALVVAIISAAFGAAVVLLLHAPAVAG
                     QTVIPGYVSSTSGTDMGASSVEQVVARVLPSVVTLQSHLGDEFEQGSGIILTPDGLIM
                     TNNHVVASGPGGRESTSSTVTFSDGRTAPFSVVATDPTSDVAVIRAQGISGLTPITFG
                     TSTGLRVGQPVIAVGSPLDLESTVTTGIISALNRPVFTPGEADNEVVVFDAIQTDAAL
                     NPGNSGGALFDMNGELIGMNSATAKLGTTGSGDTPSGSIGIGFAIPIDHAQRIERELV
                     ATGRASHGWLGAKVSNGADSQGATVVGVATPSPAAAAGLSNGALVTRVDDQPIPNAEA
                     LLAIVQSTAPGTQVKMAFFDPSGDARTVTVTLGTDPGQG"
     gene            3104..5827
                     /locus_tag="VGP27_14250"
     CDS             3104..5827
                     /locus_tag="VGP27_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEV7852494.1"
                     /translation="MTTTPGAERQRIAGFGRLDLGLHQASDWYLWGPYLSERQWGTVR
                     EDYSPDGDAWNYLPHDHARSRAYRWGEEGLAGFCDIEQRLCVALALWNGHDPILKERS
                     FGLTGAEANHGEDVKEYWWYLDAVPSHAWNSWRYHYPQRAYPYQQLLDENRRRGKLDA
                     EYELLDTGIFDDDRYWIVDVHYAKADPTDVLMTISVTNAGPDTELLHVLPTMWYRNTW
                     SWDVDGTRPTIAATSTGSVAVNHPFLGHLELLPGPDPQGGYPDLLFCDNDTNLQRLYG
                     VPSRTRWSKDGINDHVVTGADTVNPQRQGSKCAAWYRLTVAPGETAQVRLRLRPEGSG
                     PDAATALGADFTRVAAQRRAEADEFYGELTPPAASADEAAVMRQAFAGMLWNKQFYNY
                     HVARWLDGDPGQPPPPPQRREGRNARWRSFEAFDVMSMPDTWEYPWFAAWDLAFHCVA
                     LVHVDPAFAKYQLSLLCREWFQNPNGALPAYEWDFNDVNPPVQAWAALEVFAIDGGRD
                     FDFLSRIFDKLLVNFTWWVNREDANGSNLFEGGFMGLDNIGPLDRSHLPVHGLLEQSD
                     ATGWMAGYALSMATIAAILQGSGHRPALDLVQKFLEHFAGIAEALETMGLWDNHDGLF
                     YDRILLADGGAVPIRVRSMVAMIAPLAAAVVNEEVIDRTLVTDKMFADYLRRHGLGGT
                     KEMTDAGILRGQPGQRRLLVGVTGLEKVRRLCRSMFDPAEFLSPHGLRSLSAYHRDHP
                     YVLNVQGVRAGIDYEPAESTTAMFGGNSNWRGPVWFPLNYLVATALERYHEFFGDDLT
                     IEYPTGSGTNVTLDAVAADLWERLVALFLVGQDGRRPCFGGVERMQTDPRWRDNLLFH
                     EYFHGDNGAGLGASHQTGWTGLVADVIRRRHRAYPKASEVIQRLVQGEEIG"
     gene            5824..7914
                     /gene="glgX"
                     /locus_tag="VGP27_14255"
     CDS             5824..7914
                     /gene="glgX"
                     /locus_tag="VGP27_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007237708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV7852495.1"
                     /translation="MSATILPGRPAPLGATPGEHGTNFAVSSNGDAVTLSLFSGDGSE
                     TRLLLPERDGDVWHGFVPGVAPGQPYGYRVSGPYDRARGQRYNPAKLLLDPYARAING
                     DVRFGPELLDYAMDNTAASSPLDSAGQMPRSLVTAPVSTPPAPGPGHALADSILYEVH
                     VRGFTATHPGVPAELRGTYAGLAHKAAVRHLVDLGITTVELLPIHHNVPESFLVERGL
                     TNYWGYNTIGFFAPHAAYSAAVRAGRPGGQVEEFRNMVAALHTAGLEVVLDVVFNHTA
                     EGGAGGPTLCHRGLDNAAYYRLDPGDPSRYVDTTGCGNSLNTADATTLRMIMDSLRYW
                     VSEMGVDGFRFDLAPTLGREDGDFDPFSAFFDTIGQDPVVSQVKLIAEPWDVGRIDSY
                     DIGRFPPLWSEWNGRYRDTVRDWWRSHDGLLPDFATRLCGSADLYDRPDEARRPSASV
                     NFVTVHDGFTLNDLVSYTAKHNEANTEDNRDGTDDNRSWNCGAEGPTDDSDVNALRAQ
                     QKRAFLVTLLLSAGVPLLLGGDELGRTQRGNNNAYCQDNEITWFDWSNLDSELLQFTT
                     DLIALRRHHPVFRRRRYASGKEAADLRWFTPSGTAMTTENWTDPNARSVALFIDGSTD
                     PDVGPDGTPMVDNDFLMLINAWWEPLTFTVPADLAARQWSVVCDTNDPGRDAVSGDEV
                     AVEQRSLVVLRSRT"
     gene            8108..8785
                     /locus_tag="VGP27_14260"
     CDS             8108..8785
                     /locus_tag="VGP27_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7852496.1"
                     /translation="MSETRRHPTTTPAAPVRHFFEDAVSKSRFWWLLPVTGVAWIVLS
                     IVILRFNYTTVAAVAVLFGVYCLAVAVTQIMIGAVSSSTGWRIAHGLLAALLVVVGVV
                     SLANFGATFVTLAAIVSFYFIFRGCFDIVMALAGSAVSGWWVLLICGFIELGLGFWAA
                     GSWSVSVVVLVAWVAAAALVRGIGEIALGFQIHQGRRDITSVAGLADQLGVTNNHPAP
                     DAAVPGR"
     gene            8811..9404
                     /locus_tag="VGP27_14265"
     CDS             8811..9404
                     /locus_tag="VGP27_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852497.1"
                     /translation="MESVEYLHRWWVLRAGGRNPLVRASDRVELLIIVLGISAVLLAG
                     AFAGALGTAVHDARSRVYIAQGQTRHTVTARAIGDSTTVFGADGNTATRVNVRWKVDG
                     TEYTDSFNMAHAVKTGDPLRIWVDRNGDRVDAPMPTSQAGVDAFTVAYAAWQTVVLAV
                     AGLIYWGHSRLEHRRASAWEREIRSFMRASTGGHHNS"
     gene            9451..9954
                     /locus_tag="VGP27_14270"
     CDS             9451..9954
                     /locus_tag="VGP27_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7852498.1"
                     /translation="MPDFVNVLVLIGLPDGPVNRAMSRHALDTSPEGVMVNVFDDLAG
                     LPHYSEALEADGPPDAVVALRTAAAEADALLVVTSYSGRIPTMAHCAIDWLTRRWRHG
                     ALHDKPVAVIGRSNGCYSGVWSRQIEDARGDLGPRMIEPLTVPTLREAVKKLADDVHG
                     GSAAAAM"
     gene            complement(10255..10371)
                     /locus_tag="VGP27_14275"
                     /pseudo
     CDS             complement(10255..10371)
                     /locus_tag="VGP27_14275"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase"
     gene            complement(10570..10885)
                     /locus_tag="VGP27_14280"
                     /pseudo
     CDS             complement(10570..10885)
                     /locus_tag="VGP27_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778000.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(10917..>11259)
                     /locus_tag="VGP27_14285"
     CDS             complement(10917..>11259)
                     /locus_tag="VGP27_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7852499.1"
                     /translation="KRTVVATIGDGSCQYSVQAIYTAAQHRLPVVFVVMRNEEYSILK
                     SFAVLEETPGVPGLDLPGLDIASLATGFGCRTVNVDSCEKLAREFTNALDVDGPTVIV
                     VPTKPQPAHLG"
BASE COUNT         1961 a         3521 c         3596 g         2181 t
ORIGIN      
        1 cccgatcaca cactgagcgc ggcagcgttg gcgcgcgcat acatggcatc gagcaccgcg
       61 tactccgcct cctcgatcgc gtcgagagca aaatcgatcg catcgtaggc atacgactcg
      121 ctcaggtcgg catccatcgc cgctccgtcg gcgtcaatct tgtcgttgtg ctttttgacc
      181 cgcgagtgca cctcatcgac gtgtgcatgc cacttgtcgc ggagctcctg ccagtgtgag
      241 gacgcggcgt cccgggcgtc atccgccttg cccttgacgt gatcggctgc tgccgtagct
      301 ctatcccgcg cgtcgtcgac gtcgtttgcg agctgctcgc gcgtccgctc attggccgcc
      361 gatagggcgt cgctcgcagc cttcgccttg tcggagatct tgtcgaactc tgcgctcagt
      421 ttggactctg ccatcaaatg ctccttgtca atggggggcg gcctctccgc acctaagtga
      481 atcgtggggc ctgacgatgg caccgtcatt gttcagcgcg acaccgtagg gaaccgatga
      541 ttggccggag cgatatcgac agacagacgt gatgagatag ccgtcaaatg tgtcggcgtc
      601 ggcgacaccg agcaccgcgt catgttgttc gcgacgacat cgacaatggc tgcgcctcgt
      661 caaaggatgg cctggtgggc ataaagcccg cgctaccaac ggtatccgtc cggatcggac
      721 gacagggagt ggtttagatg gcgaacgcaa acgtgagacc ccttcttggg cagcagctgt
      781 ggaaatggac tctggcagcc ggtgtgctgg cgattgcctt gggcggcatc gttcttgcgt
      841 ggcccggccc gacgatcctt gtcgcttcga cgttgttcgg ggtgtacctg ctgttgactg
      901 gattgatcgg actgtccatg gcgttcacgc tgccccgatc tcccggcatg cgcgttctgc
      961 tgttcatcag cggagcgctg gcggtggtgc tggccattct gtccttccgc cacttcggcg
     1021 atgcatacgc ggttcttctg ctgtcactgt ggatcggaat tgggtttgtt ttccagggca
     1081 tctcggccac cgctgcagct atcggcgagt ccaatctgcc cggacgcggc tggtatgtag
     1141 tccttggcgt catctctgtg atcgccggcc tcgtcgtcct tgtttggccc ttcgattcaa
     1201 tcgtcgtgct tgtgctcgtt gccggcgtgt ggctcgtcgt gctcggcgtg gtacagatca
     1261 tccagtcgtt ccagatccgc aaggatgcca gaacggtccg cgaagccatt gactcagtct
     1321 ctgagcgctt cgaggccagg tgatgatgat ggactccgaa ttgaatgcac gattcgaatg
     1381 tgaggttgtt ccgctacggg cctccctgta tcgccatgcg tgtcgcatca cccggaacga
     1441 cgcagacgcg gatgacctgg tgcaggagac catgatgagg gcgtacgtcg gtttcaagtc
     1501 gttccggtca gacagcaacc tgaaggcatg gctgttccgg attctgacca acacgtacat
     1561 caacggctac cggaaggaac gacgccaacc ggtgcagtgc tcgatggagc aggtgaccga
     1621 gcagtacttg gcagcggcgt atgcccgttg cgtgcctgga cagcggtcgg tcgaggatta
     1681 tgttctcgat tcgttgccgg acaaggacat cgagatggcg atgcagaccc tgccgtcaca
     1741 gttccgcgag gtcgtctact acgccgatgt cgagggctat cgctacaaag agatcgcggc
     1801 gatgatgaag actccgcggg gaaccgtgat gtcgcggctt catcgcggcc gggagcggct
     1861 tcgggcagtg ctcactcccg ctggcggctg acgtgatgtc ggactcgaga cggggtaccg
     1921 gccggacacg tccccccgct caccgtgtgg cgtgggcaga ttggcacagt cttgttggta
     1981 aaggcagatt gctctggcgt gcgcttgtgg tggcaattat ttcggccgcc tttggtgcag
     2041 cggtcgtttt gcttctccat gctcctgcag tcgccggtca aaccgtgatt cctgggtacg
     2101 tgagctccac gtcgggcacc gatatgggcg cgtcctcggt cgagcaggtc gtcgccaggg
     2161 tgttgcccag cgtggtgacg ctacaatcgc atctgggtga cgagttcgaa caaggttccg
     2221 gcatcatcct caccccggat gggctgatca tgaccaataa tcacgttgtg gcgtccggtc
     2281 ccggcggccg cgagtcgaca agcagcacgg tcacgttcag cgacggtcgc acagctccgt
     2341 tcagcgttgt cgctaccgac cccacgagcg atgtcgccgt tattcgggcg caggggattt
     2401 caggactcac cccgatcacc ttcggtactt cgaccggcct ccgcgtcggt cagccggtga
     2461 tcgctgtggg ttcaccgctc gacctggaga gcacggtgac aacgggcatc atcagcgcgc
     2521 tgaatcgacc ggtgttcacc cctggtgaag cggacaatga ggttgtggtg ttcgacgcga
     2581 tccagaccga tgccgcgctc aatccgggta attccggtgg ggccctgttc gacatgaacg
     2641 gtgagctcat cgggatgaac tcggcgacgg cgaagctggg cactactggc tccggggaca
     2701 ccccgagcgg gtcgatcggg attggattcg ccattccaat cgatcacgcg cagcgcatcg
     2761 agcgcgaact cgtcgccacg ggaagggcgt cgcatggttg gcttggagcg aaggtcagca
     2821 acggcgcaga tagccagggc gccacggttg tcggtgtcgc caccccaagc ccggccgccg
     2881 cggcgggcct gtccaacggc gcgttggtga cgagggtcga tgaccaaccg attcccaatg
     2941 cagaggcact gttggcgatt gtgcaatcaa cggcgccagg cacacaggtg aagatggcat
     3001 tcttcgatcc atcgggtgat gccaggactg tcacggtcac tctggggaca gatccagggc
     3061 aaggctagcc cgtgttcgtc actacagcac cacagctctc gatatgacaa cgactccggg
     3121 ggcggagcgg cagcggatcg ctggtttcgg gcgcctcgac cttggtctac accaggccag
     3181 cgactggtat ctgtggggcc cgtacctgag cgaaaggcaa tggggcaccg tcagggagga
     3241 ttacagcccc gacggggacg cctggaacta tctaccgcac gaccacgcgc gctcgcgtgc
     3301 ctaccgctgg ggcgaggagg gcctcgccgg cttttgtgac atcgagcagc ggttgtgtgt
     3361 ggcgctggcg ttatggaacg ggcacgaccc gatcctgaag gagcggtcgt tcggcttgac
     3421 gggcgcggag gccaatcacg gcgaggacgt caaggagtac tggtggtatc tcgacgctgt
     3481 gccgagccac gcctggaaca gctggcgcta ccactacccg cagagggcct acccgtatca
     3541 acaactgctc gatgagaacc gacggcgcgg aaagctggat gccgagtacg agttgctcga
     3601 caccgggatc tttgacgatg accgctactg gatagtcgac gtccactacg ccaaggcgga
     3661 cccgaccgac gtcctgatga cgatcagcgt gaccaacgcc ggacccgaca ccgaactgct
     3721 gcacgtgctt ccgaccatgt ggtaccgcaa cacctggtcc tgggacgtcg atggaacccg
     3781 accgacgatt gcggccacat caaccgggtc cgtggccgtg aaccatccat tcctaggtca
     3841 tcttgagctg ttgcccggac cggacccaca gggcggttac ccggacctgc tgttctgcga
     3901 caacgacacg aatcttcaac ggctctatgg ggtgccgtct cgcacgcgat ggtcgaaaga
     3961 cggaatcaac gaccacgtgg tcacaggcgc cgacaccgtc aacccgcagc gacagggttc
     4021 caagtgtgcg gcctggtaca ggctgacggt ggcaccgggc gagaccgcac aagtgcggtt
     4081 gcggctacga ccggaaggca gtggcccgga cgcggcgacc gctctcggcg cggacttcac
     4141 acgggtagcc gcgcaacggc gggccgaagc cgacgagttc tacggcgagc tcacgcctcc
     4201 cgcggcatcg gccgatgagg ctgcggtcat gcgacaggcg ttcgccggca tgttgtggaa
     4261 caagcagttc tacaactacc acgtcgcccg ctggctggac ggcgacccgg gccagccacc
     4321 gcccccgccg cagcggcgcg agggccgcaa cgcccggtgg cgcagttttg aggcatttga
     4381 cgtcatgtcg atgcccgaca cgtgggaata tccctggttc gcagcctggg acttggcatt
     4441 tcattgtgtc gcacttgtgc acgtcgatcc cgcattcgcg aaatatcagc tttcgctgtt
     4501 atgccgcgag tggttccaga accccaacgg cgcccttcct gcctatgagt gggacttcaa
     4561 tgacgtcaac ccgccggtgc aggcgtgggc cgcgctggaa gtgttcgcca tcgacggcgg
     4621 ccgcgacttc gacttcctga gccgaatctt cgacaagctg ctggtgaatt tcacctggtg
     4681 ggtgaatcgc gaagatgcca atggctccaa cctctttgag ggtggattca tgggtctgga
     4741 caacatcggc ccgcttgacc gctcgcacct tcctgtccac gggctcctcg agcagtctga
     4801 tgccaccggc tggatggcgg gatacgcact ttcgatggcc accatcgctg cgatccttca
     4861 ggggtcgggc caccggcccg cactcgactt ggtgcagaag ttccttgagc acttcgccgg
     4921 tatcgcggaa gcgcttgaga caatgggtct ttgggacaac cacgacggac tcttctatga
     4981 ccgtatcctg ctggccgacg gcggtgccgt accgatacgc gtccgctcga tggtcgcaat
     5041 gattgcacct ctggcagcgg cggtcgttaa cgaggaggtc atcgaccgga cgctggtgac
     5101 cgacaagatg ttcgccgact acctgcgacg ccatgggctc ggaggtacca aggagatgac
     5161 cgatgcggga atcctgcggg ggcagccggg acagcgccgg ttgttggtgg gggtgaccgg
     5221 gctggagaag gtccggcggc tatgccggtc catgttcgac ccagcggagt ttctgtcgcc
     5281 gcacggactc cgttcgctct cggcgtacca ccgcgaccac ccgtacgtcc tgaatgtgca
     5341 aggcgtgcgg gccggcatcg actacgagcc tgccgagtcg accaccgcga tgttcggcgg
     5401 taattcgaac tggcgtggcc cggtgtggtt tccgctgaac tatctagtag cgaccgcgct
     5461 ggagcgatac cacgagtttt tcggtgacga cctgacgatc gaatacccga ccgggtcagg
     5521 caccaacgtc accctcgacg ccgtcgccgc cgatctctgg gagcgtctgg ttgcgctctt
     5581 tctcgtcggc caagacggcc ggcggccctg cttcggcggt gtggagcgga tgcagaccga
     5641 tccccggtgg cgcgacaacc tgctctttca tgagtacttc catggcgaca acggtgcggg
     5701 cctcggcgcg tctcaccaga cagggtggac cgggctggtc gccgacgtga tcaggcgacg
     5761 ccaccgcgcc tacccgaagg cgtcggaagt catccagcgc ctggttcagg gagaggagat
     5821 cgggtgagtg ccaccatcct tccgggccgg cccgccccgc tcggcgcgac gccgggcgag
     5881 cacggcacga actttgccgt gtcgtccaac ggtgatgcgg tcaccttgag tctcttcagt
     5941 ggcgacggca gcgaaacccg gctgctgcta ccggagcgcg acggcgacgt ctggcacggg
     6001 tttgtgcccg gcgtggcgcc ggggcaaccc tacggttatc gggtcagtgg accctacgac
     6061 cgcgcacgcg gccagcgtta taacccggcc aaactcctgc tcgaccccta tgcgcgcgcg
     6121 atcaacggcg acgtccggtt tgggcccgaa ttgctcgact acgcaatgga taacaccgct
     6181 gcatccagcc cgcttgattc agcgggtcag atgccgcgca gcctggtgac cgcccccgtc
     6241 tccacgccgc cggcaccggg gcccggacac gcccttgccg actcgatcct ctacgaggtg
     6301 cacgtgcgag ggttcaccgc gacgcatcct ggtgtgccag cagagttgcg cggcacttac
     6361 gcgggcctgg cgcacaaggc ggcggtgcga cacctcgtcg acctgggaat caccacggtc
     6421 gagctactgc cgatccacca caacgtgccc gagtcctttc tcgtcgagcg ggggttgacg
     6481 aactactggg ggtacaacac catcggcttc ttcgccccgc acgcggccta ctccgcggca
     6541 gtgcgagccg ggcgaccagg cggacaggtc gaggaattcc gcaacatggt cgccgcgtta
     6601 cacacggccg gtttggaagt ggtgttggac gtcgtcttca accacaccgc tgaaggcggc
     6661 gccggtggcc cgacgttgtg ccatcgcggc ctggacaacg ccgcctacta ccgcctcgac
     6721 ccaggcgatc cgagccgata cgtcgatacg accgggtgcg gcaattcact gaacaccgcg
     6781 gacgcgacaa cgctgcgaat gatcatggat tccctgcgct actgggtgtc cgagatgggc
     6841 gtcgacggtt tccggtttga cctggcgcca acccttggcc gggaagatgg tgacttcgac
     6901 ccgttttcgg cgttcttcga caccatcggc caggacccgg tggtctcaca agtcaaactc
     6961 atcgcggagc cgtgggatgt cggccgcatc gacagctacg acatcggtcg gtttccgccg
     7021 ttgtggtccg agtggaacgg tcgctaccgc gacaccgtgc gcgactggtg gcgcagccac
     7081 gatgggctgt tgcccgactt cgcgacgcgg ctgtgtggtt cggccgatct ctacgatcgg
     7141 cctgacgaag cacgtcggcc cagcgcgtcg gtgaacttcg tcaccgtgca cgatggtttc
     7201 accctcaacg atctagtgtc ctacaccgcc aagcacaacg aggccaacac cgaggacaac
     7261 cgcgacggca ccgacgacaa ccggtcctgg aactgcgggg cagagggccc gaccgacgac
     7321 agcgatgtga atgccctgcg ggcgcagcag aagcgggctt tccttgtgac gctgctactt
     7381 tcggctggcg taccgttgtt gctcggtggc gacgagctcg ggcgcaccca gcggggcaac
     7441 aacaatgcgt actgccagga caacgagatc acgtggttcg actggtcgaa cctcgacagt
     7501 gagctgttgc agttcaccac ggacctcatc gcgctgcgcc gtcatcatcc agttttccgc
     7561 aggcgccgtt acgcctccgg caaagaagcc gcggacctgc gctggttcac accttcgggt
     7621 accgcgatga cgaccgagaa ctggaccgac ccgaatgccc gcagcgtcgc gttattcatc
     7681 gacggctcaa ccgatcccga cgtcggcccc gacggcacgc cgatggtcga caacgacttt
     7741 ctcatgctga tcaacgcatg gtgggagccg ttgacgttca cggtcccggc agacctcgca
     7801 gcgcggcagt ggagcgtcgt gtgcgacacc aacgatcccg gccgcgacgc cgtctccggc
     7861 gatgaagttg ccgtcgagca gcggtcgttg gtcgtcctgc gctcgcggac ctgacgtctg
     7921 aacttcggtt gtcaccgatc aatcccggat agacacgccg ttgtgggcgt tgttgatcgg
     7981 cactccatcg cgacagcgac gttgtgtttc accacatcgc aattgtttgc ggttcagagc
     8041 acccttgacg taccgccgcc gtagagggtt ggcgagtctt catcaaatcg aatgcatggg
     8101 agtaccaatg agcgagaccc gtcgtcaccc caccaccaca ccggctgcgc cagttcgtca
     8161 tttcttcgaa gacgccgtgt ccaagagtcg gttctggtgg ctgctaccag tcaccggcgt
     8221 cgcctggatc gtgttgtcca tcgtgatcct gcgcttcaac tacaccaccg tcgccgcggt
     8281 ggccgtgttg ttcggggtgt actgcctggc cgtcgcggtc acccagatca tgatcggggc
     8341 ggtgtcctcg tccacgggct ggcgcatcgc gcacggcctc ctggcggcgc tgctggttgt
     8401 ggtcggagtg gtgtcgcttg ccaacttcgg cgccaccttc gtcacgctgg ccgccatcgt
     8461 cagcttctac ttcatcttcc gcggctgctt tgacattgtc atggcccttg ccggcagcgc
     8521 cgtgtccggg tggtgggtgc tgttgatttg cgggttcatc gaactgggat taggtttttg
     8581 ggccgccggg tcgtggagcg tctccgtcgt ggtactagtg gcttgggtgg ccgccgcggc
     8641 gctggtgcgc ggcatcggcg aaattgccct cggcttccag atccaccaag gacgccgcga
     8701 catcacgtcc gtggcggggc tagctgatca actcggggtc accaacaacc accccgcgcc
     8761 cgacgcagcc gtgcctggtc gttgacacga gtctcgacga gggagtggtc atggaaagcg
     8821 tcgaatatct ccatagatgg tgggttctgc gggcaggcgg ccgcaatccg ctcgtgcgtg
     8881 ccagcgaccg ggtcgaactg ttgatcatcg tgctgggaat ctcggcggtt ctgctcgccg
     8941 gcgcctttgc gggggcgctc ggcacggccg tgcacgatgc gcgtagccgc gtgtacatcg
     9001 cgcagggcca gacgcggcac accgtgaccg ccagagccat cggtgacagc acaaccgtgt
     9061 tcggagccga cggcaacacg gcaacgaggg tgaacgtccg atggaaagtc gacggcaccg
     9121 aatacaccga cagcttcaac atggcacacg ccgtcaaaac tggcgatccg ctgaggattt
     9181 gggtggaccg caatggcgac cgcgtcgatg cgcctatgcc gacatcacag gccggcgtcg
     9241 acgcgttcac tgtcgcctac gcggcatggc aaacggtcgt gcttgccgtg gccgggttga
     9301 tctactgggg acactcgcga cttgagcatc ggcgggcttc tgcctgggaa cgcgagattc
     9361 gaagtttcat gcgcgcctca acgggcggac accacaactc atgagtgcag acaggttgca
     9421 atgatggcgg ctccacaagc accaaatcca ttgcccgact tcgtcaacgt gctggtcttg
     9481 attggactcc ccgacggtcc tgtgaaccgt gcgatgagcc gacacgcgct ggacacctcg
     9541 cctgagggag tcatggtgaa tgtgttcgac gaccttgccg gcctgcccca ctacagcgaa
     9601 gccctggaag ccgacggccc gccggacgct gtcgtcgccc tacgtactgc cgccgccgag
     9661 gctgatgcgc ttctcgtcgt cacgagctac agcgggcgga tcccaacgat ggcgcattgc
     9721 gcgatcgact ggctgacgcg ccgctggcgt cacggtgcac tccatgacaa gccggttgcc
     9781 gtgatcggcc gatcgaacgg gtgctacagc ggcgtctggt cgcgtcagat tgaggatgcc
     9841 cgtggtgatt tgggtccgcg aatgatcgag ccattgacag taccgacttt gcgcgaagcg
     9901 gtcaaaaagc tcgcagatga cgtgcacggt ggcagcgctg ctgcagcgat gtgacgggcg
     9961 cttctcacct tgcacatcaa cctccggcaa gccactcatt ttcttgaccg ggctccggca
    10021 gcacacgacc gccttccact gcgaagcgag ctgggctggg cgcaatgacg acgagcactc
    10081 tgtccgggtc gatatcgcag atttcggaaa cagcggtggc gatttcggtg gccagccttg
    10141 tggtgtcgtc agtgggtggc cttcacgcac ccagccattg accaacaccg gactcgctgg
    10201 cacgccgtcg gtgtagagac cgtcggccgc caactcgtgg aatgcgacgt tcacgaacgt
    10261 gctgggcacg tgggtgatcg atgaatgaat gcgcgcgacg tcgccggcca acgcacgctt
    10321 ggtgtcggcg ttgaaggtcg attcggccat ggtgcaggtg tagatgggca tcgctactaa
    10381 tcctttgttc ggttggaggt tgcaggccgg cgtggcgggc agggtcgcat cgtgagattc
    10441 gcttgcggtg tcgggatcca atgacagcgc gaactggtcg tgccaacgaa actcgaaccg
    10501 cgccctcgac agtgcgtcgt cgcgctccta cgagcgggga tgtcccttgg ccaagtttgc
    10561 tgcgtgcgcc tcttcggcga ttgagctggt gacgaccgaa ttgccaatgt tggcattggt
    10621 tttcaccgcg aacgccttgc cgatgatcat cggctcactc tcggggtggt tgtggttgac
    10681 ggggatcccg cccgaccgac ggccacctcg tagcgcacca gctcggcggc accacgccct
    10741 ccctcgcagc gatgaatgtc acctccgcgg tgatctcacc gttggaaagg ctcacccgcc
    10801 gaaacggcac cccgtcaaca tagatcttcg tgctgcccgc aatggggccg gtggtcacag
    10861 accggtcgac aacgatctca accatcacat aactccctat tggtgtgaga tcggcgtcag
    10921 cccagatgtg ccggttgtgg ttttgtgggt accacgatca cggttggccc gtcgacatcc
    10981 agcgcgttcg tgaactcgcg cgcgagtttt tcgcagctgt cgacattcac cgtccggcat
    11041 ccgaaccctg tggcaaggga ggcgatgtca agtccaggta ggtccagacc tggaacacct
    11101 ggtgtttcct cgagcaccgc gaacgacttg agaatcgagt actcctcgtt gcgcatgacg
    11161 acgaacacga ccggcagcct gtgctgggcc gccgtataga tcgcctgcac cgagtactgg
    11221 catgacccgt cgccgatggt cgccactacc gtccgcttg
//
LOCUS       DASYLX010000597         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2551943,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000597 DASYLX010000000
VERSION     DASYLX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551943"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="cysC"
                     /locus_tag="VGP27_14290"
     CDS             <1..951
                     /gene="cysC"
                     /locus_tag="VGP27_14290"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV7852500.1"
                     /translation="GDLIARPNNQPRVAQDFDATVCWMADENSLEPGRDYIIKHTTRT
                     TRVRVTALDYRLDVNTLHRDKTATALKLNELGRVSLRSQVPLLLDEYSRNAATGSFIL
                     IDPNTNGTVAAGMILPYVSARTSSPNTVRHESLCTAENRLTKGRTVWFTGLSGSGKSS
                     VAMLVEQRLLEKGTPAYVLDGDNLRHGLNADLGFSMADRAENQRRLAHIAAILADSGQ
                     IVLVPAISPLAEHRALARQVTTEAGLDFFEVFCDTPIEDCERRDPKGLYAKARAGEIT
                     HFTGIDSPYQRPKKPDLRLTPDRTPEELADLVIELLESRR"
     gene            948..1688
                     /locus_tag="VGP27_14295"
     CDS             948..1688
                     /locus_tag="VGP27_14295"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEV7852501.1"
                     /translation="MTDPSDDEVAARLATEAGALLLGVREELADASAADRKAAGDKRS
                     HDFLMQALDRERPDDAVLSEEGVDDPIRLSSERVWIVDPLDGTREFSELGRDDWAVHV
                     ALWQGGKLVAGAVALPAQGITLATPDVAAPPAAPAKPRIVVSRTRPPAVALQVRDALD
                     GVLVEMGSAGAKVASVVLGLSDVYVHAGGQYEWDSAAPVAVARAAGLHTSRIDGSPLL
                     YNQGDVLLPDLVVCRPELADAVLAVTSG"
     gene            1797..2075
                     /locus_tag="VGP27_14300"
     CDS             1797..2075
                     /locus_tag="VGP27_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852502.1"
                     /translation="MSSKMLMRRTVAVAGGGALITLGALSAGCSHNGEQAPSTSTTTT
                     TTTATTPSSAPPASPTEKVINPTGGNLFTPGVKAPPAPTEPPGVHRNN"
     gene            2131..2385
                     /locus_tag="VGP27_14305"
     CDS             2131..2385
                     /locus_tag="VGP27_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852503.1"
                     /translation="MTLACGAAVIAIAGLATACNSSGQQSPSTSTTTATTTSPSSSTS
                     PSPTEKAVNPTGGNLFTPGNTANPAPNVPPGQHPGINNVP"
     gene            2413..3012
                     /locus_tag="VGP27_14310"
     CDS             2413..3012
                     /locus_tag="VGP27_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7852504.1"
                     /translation="MSEPTTLRALAEMPRSPAGLAESTLVMIDCQNTYTRGVMELDGV
                     QAALDEAAALLDRARSAGIPIIHIAHSDGPGSLYDIDGESGAIVDRVAPRDGEPVVVK
                     SYPNSFVQTDLDQRLKAANASNLVLAGFMTHMCVNSTARGAFNLGYAPTVVAAATATR
                     ALPGVDGDPVPASTLHSATLAAIADLFAVVVARTSEIPD"
     gene            3024..3689
                     /locus_tag="VGP27_14315"
     CDS             3024..3689
                     /locus_tag="VGP27_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7852505.1"
                     /translation="MHTTTPSTKVSRLGEKQSTSRSGLDELLDSTPLATVALIRDGHP
                     VIFPTGFARIDDQFVIHGSTGSPWMRALATGAPAAVSVTALDGVVVARSGFESSFRYR
                     SAVVFGVFEQVPDADKIRYLERLTDAFIPGRTAELRSSTRKELAATLPLRLPIGADNW
                     SLKVSAGWPEDPEEDVAGDAWAGVIPMSVAYGAPLPAPDLRPGIPVPGSVRAMTGGQS
                     SVQ"
BASE COUNT          639 a         1322 c         1260 g          598 t
ORIGIN      
        1 ggcgacctga tcgcgcggcc gaacaatcag ccgcgggtgg cgcaggattt cgacgccacg
       61 gtgtgctgga tggccgatga gaactcattg gagcccggcc gcgactacat catcaaacac
      121 acgacgcgga caacccgtgt cagggtgacc gcgctcgact accgcctcga tgtcaatacg
      181 ctgcaccgcg acaagacggc caccgcgctc aaactcaatg agctgggccg cgtctcgctg
      241 cgttcgcagg tgccgctgct gctcgacgag tacagccgca acgcggccac cggttcgttc
      301 atcctgatcg acccgaacac caacggcacc gtcgcggcgg gcatgatcct gccgtacgtg
      361 tcggcgcgca cctcgagccc caacacggtg cgccacgaat cgctgtgcac cgcggaaaac
      421 cggctgacga agggcaggac ggtgtggttt accggcctgt cgggctcggg taagtcctcg
      481 gtggcgatgc tcgtcgaaca gcgccttctg gaaaagggca ctcccgcata tgttctcgac
      541 ggagacaacc tccgtcacgg cctcaacgcc gacctcggct tctccatggc cgaccgggcg
      601 gagaaccagc gcaggctcgc acacatcgcc gccatcctcg ccgactcggg ccagatcgtg
      661 ctggtgcccg cgatcagtcc gctggcggaa caccgtgcgc tggcgcgcca ggtgaccacc
      721 gaggccggac tcgatttctt cgaggtgttc tgcgataccc cgatcgagga ctgcgaacgc
      781 cgcgacccga agggcttgta cgccaaggcg cgcgccggcg agatcaccca cttcaccggt
      841 atcgacagcc cctaccagcg gccgaagaag cccgatttga ggctcacccc tgaccgcacg
      901 cccgaggagc tcgccgatct ggtaatcgag ttgctggaaa gccgtcggtg acggatccca
      961 gcgacgacga agttgccgcg cggctggcca ccgaagccgg ggcactgctc ctcggcgtgc
     1021 gcgaggagct cgccgacgcc agtgcggccg accgaaaggc ggcgggggac aagcggtccc
     1081 acgacttcct gatgcaggcc cttgatcgcg agcgtcccga cgacgcggtg ctgtcggagg
     1141 aaggtgtcga cgaccccatc cggttgagtt ccgagcgggt gtggatcgtc gacccgctcg
     1201 acggcacgcg tgaattctcc gagctcggtc gcgacgactg ggcggtgcac gtcgcactct
     1261 ggcagggcgg caaactcgtt gcgggtgcgg tggcgcttcc ggcgcaggga atcacgcttg
     1321 ccacgccgga cgtcgctgcc ccgcctgctg caccggccaa gccgcgcatc gtcgtctccc
     1381 ggacccgtcc gcctgccgtc gcactccagg ttcgtgacgc gctcgacggt gtgctcgtcg
     1441 aaatgggttc cgcaggagcc aaagtcgcgt cggtcgtgct gggcctttcc gatgtctatg
     1501 tgcacgcggg tgggcagtac gaatgggatt ccgcagcgcc ggtcgccgtc gcgcgcgccg
     1561 ccggactgca cacctcgcgc atcgacggct ccccgctgct ctacaaccag ggcgatgtgt
     1621 tgctgccaga tctggtggtg tgccgacccg aactcgccga tgcggtcctc gcggtcacgt
     1681 ccggctagga attcccacag cgtggcctgc cggtcagctg agtgtcggct gcgagttggt
     1741 ttggagatgc ctgcatcgcc ttgtggacct tagttgtctg actaattgtg gtgatcatga
     1801 gctcgaaaat gttgatgcgc cgcacggtcg ccgtagccgg cggtggggcg ctcattacgc
     1861 tgggggcact cagcgcaggc tgtagccaca acggcgagca ggcgccttcc acgtcgacga
     1921 ccacgacgac gacgaccgcg acgacacctt cgagtgctcc gccggcgtcg ccgacggaga
     1981 aggtcatcaa cccgaccggc ggcaacctgt tcacacccgg cgtcaaggca ccgcctgcgc
     2041 caacggaacc gcccggcgtg caccggaaca actaagagct cacgaatggg acgaagcatt
     2101 ttgcataccg tcaaggacag tcggttgggg atgaccctcg cctgcggcgc agcggtgatc
     2161 gcgatcgcgg gcctggccac cgcctgtaac agcagcggcc aacaatcccc gtcgacgtcg
     2221 accaccacgg cgacgaccac gtcaccgtcg agctcgacct cgccgtcgcc gacggagaag
     2281 gccgtcaacc cgaccggcgg caacctgttc acaccaggga acacggcgaa tcccgctcct
     2341 aatgtgccgc cggggcagca tccaggcatc aacaacgtcc cgtaaggcgg cctgaccgcc
     2401 tacgctcggc gggtgtctga acccacgacc ctgcgtgccc tggccgaaat gccccgctca
     2461 ccggccggcc tggccgagtc gacgctcgtc atgatcgact gccagaacac ctacacccgc
     2521 ggtgtgatgg aactcgacgg agtacaggcg gccctcgacg aggcggcggc gctgctggat
     2581 cgggcccgct ccgcgggcat cccgatcatc cacattgcgc actccgacgg tcccggctcg
     2641 ctctatgaca tcgacggcga gagcggggcg atcgtggatc gcgtcgcccc gcgcgacggc
     2701 gagccggtgg tggtgaagag ctacccgaac tcgttcgtgc agacggatct tgaccagcga
     2761 ttgaaggcgg cgaacgcgtc gaatctcgtg ctcgccggat tcatgacaca catgtgcgtg
     2821 aactccacgg cacgcggggc gttcaacctg gggtacgcgc cgacggtggt ggccgccgcg
     2881 accgccacca gggcgctgcc cggcgtcgat ggcgatccgg tgcccgcatc gacgctgcac
     2941 tcggcgaccc tggccgcgat cgccgacctc ttcgccgtcg tcgttgcgcg cacgtcggaa
     3001 atcccggact gacgggtccg gcggtgcaca cgacgacacc ctcgaccaag gtcagccggc
     3061 tcggcgagaa gcagagcaca tcgcgcagcg gactcgacga gcttctcgac agcacaccgc
     3121 tggccaccgt ggcgctcatc cgcgacggtc atccggtgat cttccccacc ggcttcgcca
     3181 ggattgacga ccaattcgtg atccacgggt ccaccggctc accgtggatg cgcgcgttgg
     3241 ccacgggcgc gcctgccgcc gtctcggtca cggccttgga cggagtcgtg gtggcgcgca
     3301 gcggctttga atcctcattc cgctatcgca gcgccgtcgt cttcggcgtc ttcgagcagg
     3361 tgcccgatgc cgacaagatc cggtacctgg agcggctgac cgacgcgttc atccctgggc
     3421 ggaccgcgga actgcggtcc agcacgcgta aggagctggc cgcgacactg ccgctgcggc
     3481 tgcccatcgg cgcggacaac tggtcgctga aggtcagcgc cggttggccg gaggacccgg
     3541 aggaggacgt cgcaggcgac gcatgggcgg gtgtcatccc gatgtctgtc gcctacggcg
     3601 ccccgctgcc cgcacccgac ctgcggcctg gcatcccggt gcccggatcg gtgcgcgcga
     3661 tgaccggcgg gcagtcgtca gtccaataac gcggtgcggc ggtaaagatc cgttcggcgg
     3721 tcgccgagca cgtcgttgtg cgcgttgatg cgcttgaccc gcgattcggc gaggtcgaca
     3781 tcggcggtga cgacggccgc cgtgccgaac ctggcggtg
//
LOCUS       DASYLX010000598         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_798077,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000598 DASYLX010000000
VERSION     DASYLX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798077"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VGP27_14320"
     CDS             <1..1089
                     /locus_tag="VGP27_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEV7852506.1"
                     /translation="LWEFHFAEGMADDRFALIGKVHHTLADGVASANLLARLMDLAGS
                     AQDERDDYTTCALPSRAELLQSAGRDHVRNVAALPGLMRDAVRGFTRVRRRSREHGDH
                     PEMAKLFKAPPTFLNHVISPVRTFGTASLSLAEVKETAKHLGVTFNDIVLAMAAGGLR
                     ELLLRYDGRADRPILASVPVATDRSTDRVTGNEIGGLSVSLPVHIDDPLERVRLTSVA
                     ASRAKENYELMGPELQGKLMQYLPPSFAPALFRWQSKRAAHNSIMNVAVSNVPGPRER
                     GHIGGAPVNEIYSIGILSPGSAFNMTVWSYVDQLDISILSDDRTFDDIHEATDAMVHG
                     LAEIRLAAGLPEELSTVGTAMAPAKAVS"
     gene            complement(1086..1898)
                     /locus_tag="VGP27_14325"
     CDS             complement(1086..1898)
                     /locus_tag="VGP27_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7852507.1"
                     /translation="MGDKDVDSIDKGEATKWDPTFTEQVNNTIGPLIKRYFRAEVDGM
                     DAVPAAGGVLIVSNHSGGMFTPDVLVLAPAFYNKFGFDRPLYTLGHYGIFIGPMAGWL
                     RRVGVIEANRENAAKALRSGAVVLVFPGGDYDSYRPSFENKIDFAGRTGYVRTAIESG
                     VPIVPVVSIGGQEGQLFITRGNWLAKRLGLERFRAEILPVSFGFPFGFSVFFPPNVPL
                     PTKIVTRVLDPIDVVKEFGEEADVDEVDAHVRRVMQTALDKLAKKRRFPILG"
     gene            2026..>2679
                     /locus_tag="VGP27_14330"
     CDS             2026..>2679
                     /locus_tag="VGP27_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852508.1"
                     /translation="MTDAVLVTGGFGLVGTETVKQLAAAGRRVVVADLGTPANRKKAK
                     ALPDGVATRWADLTDAEEVDRLVSEVAPSVIVHLAAVIPPPLYRNPGLARKVNVEATK
                     AMVRAAEAQQNPPRFVQASSNAVYGSRNPHRVTDVVRADTPPRPFELYSNTKFEAEEF
                     VRTSSLEWVVLRLGGVLSPNFSALPLSGDGIFIESALPTDGRIHTVDVRDVGAAFAAA
                     "
BASE COUNT          481 a          888 c          847 g          463 t
ORIGIN      
        1 ctgtgggaat ttcacttcgc cgaagggatg gccgacgacc gcttcgcgtt gatcggcaag
       61 gtgcaccaca ccttggccga cggagtcgcg tcggccaatc tcctcgcccg gctgatggat
      121 ctcgccgggt cggcgcagga cgagcgcgac gactacacca cgtgcgccct gccgtcgcgg
      181 gccgagttgc tgcagagcgc agggcgtgac cacgtccgga acgtcgcggc gctgcccggc
      241 ctgatgcgcg acgccgtccg cgggttcact cgcgtccggc gccggtcgcg cgagcacggc
      301 gatcatcctg agatggcgaa gttgttcaag gcgccgccga catttctcaa ccacgtcatc
      361 tcccccgtcc ggaccttcgg gacggcgtca ctgtcgctgg ccgaggtcaa ggagacggcc
      421 aaacacctcg gcgtgacgtt caacgacatc gttttggcaa tggctgcagg aggattgcga
      481 gaactattgc tgcgctacga cggtcgcgcc gaccggccga tcctggcgtc ggttccggtg
      541 gccacggaca ggtcgacgga tcgggtcacc ggcaatgaga tcggcgggtt gtcggtgtca
      601 cttcccgttc acatcgacga tccgctcgag cgggtgcggc tgacgtcggt tgcggcgtcg
      661 cgcgcaaagg agaactacga actgatgggc ccggaactgc agggcaagtt gatgcagtac
      721 ctgccaccgt cgttcgcgcc ggcgttgttc cggtggcagt ccaagcgcgc cgcccacaac
      781 agcatcatga acgtcgcggt gtccaacgtg cccgggcccc gcgaacgcgg acacatcggc
      841 ggcgccccgg tcaacgagat ctactcgatc ggaattctgt cgccgggcag cgcattcaac
      901 atgaccgtct ggagttacgt tgaccagctg gacatctcga tactctccga cgaccgcacg
      961 ttcgacgata tacacgaagc gacggacgcg atggttcacg gcctggcaga aatccgcctc
     1021 gccgcagggc ttcccgagga gctcagcacc gtcggcacgg cgatggcgcc tgcgaaggcg
     1081 gtgagttagc ccagtatcgg gaagcggcgc ttcttggcca gcttgtcgag cgcggtctgc
     1141 atcacccgac ggacatgagc gtcgacctca tcgacgtcgg cctcctcgcc gaactccttc
     1201 accacgtcga tggggtccaa cacccgcgtg acgatcttgg tgggcagcgg gacattgggc
     1261 gggaagaaca cactgaagcc gaacgggaag ccgaaactca ccggcaggat ctcggcccga
     1321 aagcgttcca agcccaatcg cttggccaac cagttgccac gggtgatgaa caactgccct
     1381 tcctgaccgc cgatcgacac caccggcacg atcggtacac ccgattcgat cgccgtccgc
     1441 acgtagccgg tcctgccggc gaagtcgatc ttgttctcga acgacggccg gtaggagtca
     1501 tagtcgccgc cgggaaacac cagcacgacc gctccggatc gcaatgcctt cgcggcgttc
     1561 tcccggttcg cctcgatgac gccgacgcgg cgtaaccacc ccgccattgg cccgatgaag
     1621 atcccgtagt gaccgagcgt gtacagcggc cggtcgaagc cgaacttgtt gtagaacgcg
     1681 ggtgcgagca cgaggacgtc aggtgtgaac atgccgcccg agtggttcga cacgatcagc
     1741 acaccgcctg ccgcaggcac ggcgtccatt ccgtccacct cggcgcggaa gtaccgcttg
     1801 atcagcggcc cgatggtgtt gttcacctgc tcggtgaagg tcgggtccca cttcgtggcc
     1861 tcgcccttgt cgatggagtc aacgtctttg tcgcccatgg cttcctcgcg tcctattgtg
     1921 tgatccacat acccaaaacg cgcgtgcagt atccgctacc tgggcttacc agcggatatg
     1981 gtactctctc caattgagaa tgtcatttcc atcagagggg aagagatgac ggacgcggtt
     2041 ctcgtcacgg gtgggttcgg gttggtgggg accgaaaccg tcaaacaact ggccgctgcg
     2101 ggccgccggg tggtggtcgc cgacctcggc acccccgcca accgcaagaa agccaaggca
     2161 ttgcccgatg gcgttgcgac acgatgggcg gacctcacgg atgccgagga ggtcgatcgc
     2221 ctcgtttcgg aggtggcgcc atcggtcatc gtccatctcg cggctgtcat tccgccgccg
     2281 ctgtaccgga accccggtct ggcccgcaag gtcaacgtcg aggccacgaa agcaatggtg
     2341 cgcgccgccg aggcgcaaca gaatccgcct cgcttcgtgc aagcttcgag caacgccgtc
     2401 tacggctccc gcaatccaca ccgagtgacc gatgtggtgc gcgccgacac cccgccgcgg
     2461 cccttcgagc tctacagcaa cacgaaattc gaggccgagg agttcgtccg cacttcgagc
     2521 ttggagtggg tggtgctacg actcggcggt gtactcagcc cgaatttcag cgccctgccg
     2581 ctcagtggcg acggcatctt catcgagagc gcgctgccga ccgacggccg gattcacacg
     2641 gtcgacgtcc gcgacgtcgg cgccgccttc gcggcggcc
//
LOCUS       DASYLX010000599         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_29910, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000599 DASYLX010000000
VERSION     DASYLX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29910"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="serC"
                     /locus_tag="VGP27_14335"
     CDS             <1..625
                     /gene="serC"
                     /locus_tag="VGP27_14335"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEV7852509.1"
                     /translation="DALIVIDATSAAGGLPVDITDADAYYFAPQKNFASDGGLWIAVV
                     SPAALARIESIAASGRWVPDFLSLPIAVENSLKNQTYNTPAIGTLILLAEQLDWMQAN
                     GGLDWAVKRTADSSQRLYSWAEASQFATPFVADPEHRSQVVGTIDFSDDVDAAVVAKV
                     LRANGIVDTEPYRKLGRNQLRIGMFTAVEPDDVTALTRCVDWVVERL"
     gene            734..1507
                     /gene="sepH"
                     /locus_tag="VGP27_14340"
     CDS             734..1507
                     /gene="sepH"
                     /locus_tag="VGP27_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEV7852510.1"
                     /translation="MRELKVVGLDIDGKRIICESDDPNEKFILRADDRLRAAVRGEKS
                     VASQPQSDVEVPNVLSPKEIQARIRAGASVEQVAESAGVDIARVERFAHPVLLERSRA
                     AELATAAHPVLADGPAVLTLLETVTSALIARGLDPDSVNWDAWRNEDGRWTVQLSWLA
                     GHSQNTAHFRFTPGAHGGTVTAFDDAACALIDPDFARPLRPVAPLAQLAIDEPEPPLQ
                     VQEEPEPPKTARTPRPRKGKPPVPAWEDVLLGVRSSGQR"
     gene            complement(1504..>1708)
                     /locus_tag="VGP27_14345"
     CDS             complement(1504..>1708)
                     /locus_tag="VGP27_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2537 domain-containing protein"
                     /protein_id="HEV7852511.1"
                     /translation="AGAVVVLSTGLIRVHPLLAVGLNLVAVGGLAPTVWGWRATPVLR
                     WFVLGLGVGVAGAWLALLALSLG"
BASE COUNT          295 a          615 c          551 g          247 t
ORIGIN      
        1 cgacgcgctc atcgtcatcg acgccacctc ggcggcgggc ggcctcccgg tggacatcac
       61 cgacgccgac gcgtattact ttgcgccgca gaagaatttc gccagcgacg gcgggttgtg
      121 gattgcggtc gtctcccccg ctgcactcgc gcgcatcgag tcgatcgccg cttccggacg
      181 ctgggtgccg gacttcctgt cgctgcccat cgccgtcgag aacagcctga agaaccagac
      241 ctacaacacc ccggccatcg gcacgctgat cctgcttgcc gagcagctcg actggatgca
      301 ggccaacggc gggctggact gggccgtcaa gcgcaccgcg gactcgtcgc agcggctcta
      361 ctcctgggcc gaggcgtcgc agttcgccac gccgttcgtc gccgatcccg agcatcgttc
      421 acaggtggtg ggcaccatcg acttctccga cgacgtcgat gccgcggtgg tggccaaggt
      481 gctacgggct aacggcatcg tcgacaccga gccataccgc aaactcggcc gcaaccagtt
      541 gcggatcggc atgttcaccg ccgtcgaacc cgacgacgtg accgcgttga cgcgatgcgt
      601 cgattgggtg gtggaacggc tctgacctgg gtagctcggg cgtgtcgggc gggtaacgcg
      661 ccgataacgc agtagggtga cctgctaatc gggcacggcg tcccggcgcc aatgaagccc
      721 ggaggaggtc ggcatgcggg aactcaaggt cgtcgggctc gacattgatg gcaaacgcat
      781 catctgcgaa agcgacgacc cgaacgagaa gttcattctg cgcgccgacg accggttgcg
      841 ggccgctgtg cgcggcgaga agtctgttgc cagccaaccc caatccgatg tcgaggttcc
      901 aaacgtgctg tctcccaagg agattcaggc cagaattcgc gcaggcgcgt ccgtcgagca
      961 ggtcgcggaa tcggcaggcg tcgacatcgc ccgtgtcgag cgcttcgcgc atcccgtcct
     1021 gctcgaacgc tcgcgcgcgg ccgaactggc aactgctgcg catcccgttc tcgccgacgg
     1081 ccccgcggtg ctcaccctgc tggagaccgt gacgtcggcg ctgatcgccc gcggcttgga
     1141 tccggactcc gtcaactggg acgcatggcg aaacgaggac ggccgctgga cggtgcagct
     1201 gtcctggctg gccggtcact cacagaacac cgcgcacttc cggttcacgc ccggcgcgca
     1261 cggcggcacg gtgaccgcat tcgacgacgc cgcctgcgcg ctgatcgatc cggacttcgc
     1321 ccggccgctg cgtccggtgg cgccgctggc gcaactggcg atcgacgagc ccgagccacc
     1381 gttgcaggtg caagaagagc ccgagccacc gaaaaccgcc cgcacccccc gcccccgcaa
     1441 gggcaagccg cccgttccgg cgtgggaaga cgtgctgctc ggagtgcgct ccagcgggca
     1501 gcgctagccg agactcaacg ccagcaaggc cagccacgcg cctgccacgc cgacgccgag
     1561 acccaggacg aaccaccgca agaccggcgt cgctcgccac ccccacaccg tcggcgccag
     1621 acccccgacg gccaccaggt tgagtccgac ggccagcagc ggatgaacgc ggatcagtcc
     1681 cgtgctcaac accaccaccg cgcccgcg
//
LOCUS       DASYLX010000600         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_688964,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000600 DASYLX010000000
VERSION     DASYLX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688964"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /locus_tag="VGP27_14350"
     CDS             complement(<1..2600)
                     /locus_tag="VGP27_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7852512.1"
                     /translation="MTSDLLTGRDSELGAIRRALSGTGNYSGVVICGAAGVGKTRLAR
                     EVLARAQAAGERTNWITGTESARPLPLGAFTGSIAETASGTMPDVGRLINSFVAQQRQ
                     GKVLIGVDDAHLLDGLSAHVVHQLAQTRGPRLVVTMRTGEAEPDAVTALWKDGLLARL
                     DLEPLSEAGTRSLIESTLDGAVDARSSQRFWKLTGGNALFLRQLVTDQVAAGRMRRVA
                     GVWMWDDRVAVSQNITDMVGRQLGELNEGVALVVDTLSQCEPLAVDVLCTVADRSDLE
                     AAEQMRLVTVERSGDERMARLAHPLYGELRRAGAGEMYLSRIRGKLAQGLGAKPDADM
                     QSTVRRALLTLESDLPPDPDLFVDAARFAMTLLDLDLADRFACAAVSAGAGEAPGVQA
                     MNLLLRGRGEDAEKFLKEITADGHEDAHRWSTVRAANLIWTLGRPGDAAAILEGLTAG
                     PETPTQAASRAAVEACVDAVYGRCELAAEKATAALDAQQLGDFHAMMASFALVMALGA
                     LGRGDELTAVADRALDRSSTSFQTAPLRFWFGGVYARACRLTGRIDDCAKLAKQMADS
                     VNDVPGLAYANLVFLLGQSDLMRGELPGAVKLLHEALAGVEHHGITTGLRPATCFALA
                     EAHAKLGQSAEANAALDEARQNVPDSYVYMHTALAIATGWSLAAGGYLTDAVATVREA
                     AVNARDRGQPTHELICLQAIAQWGDASLSDRARELADELSLPLADAVARHSEALAAKD
                     GEGLLAASNEYQALGDRATAADAASQAAVAFSQSQLRRRRLYAAAIARGLADECGGLS
                     TPALRSPTGHPLTERQREIVELAVAGMSNKDIAKRLVMSVRSVEGHLYRACQRVGANS
                     REELAAIIRKG"
BASE COUNT          429 a          943 c          863 g          418 t
ORIGIN      
        1 cctttcctga tgatggcggc cagctcttcg cgcgaattgg cgcccacccg ctggcatgcg
       61 cgatacaggt gtccctccac gctgcggacc gacatgacca ggcgcttggc gatgtccttg
      121 ttcgacatac cggcgacggc caactcgacg atttcgcgtt gccgctcggt cagcgggtga
      181 ccggtcgggc tccgcaacgc gggcgtactg agaccaccgc attcgtcggc cagcccccgg
      241 gcgatcgcgg cggcatacaa cctgcgcctg cgcaactgac tctgggagaa tgccaccgcc
      301 gcctgcgagg cggcatcggc ggccgtcgcc cggtcgccga gcgcctggta ctcgttcgac
      361 gctgcgagca gcccctcgcc gtccttggcc gcaagggctt cggaatggcg ggcgacggca
      421 tcagccagcg gcaacgacaa ctcgtcggcg agctcacgag cgcggtccga cagggaggcg
      481 tctccccact gcgcgatcgc ctgcaggcag atcaactcgt gggtcggctg gccgcggtcg
      541 cgcgcattca cggcggcttc gcggacagtc gcaaccgcat ccgtcagata gccgccggcc
      601 gcaagcgacc accccgtcgc gatcgcgagg gcggtatgca tgtacacgta gctgtccggt
      661 acgttctgac gcgcttcgtc gagcgccgca ttcgcctccg ccgactggcc cagtttggcg
      721 tgcgcctcgg ccagcgcgaa gcaggtagcg ggccgcaaac ccgttgtgat gccgtgatgt
      781 tcgacaccgg cgagcgcttc gtgcagaagc ttcaccgcgc cgggcagctc tccgcgcatc
      841 aggtccgact gccccaaaag gaagaccagg ttcgcgtagg ccagacccgg cacgtcgttc
      901 acggagtcgg ccatctgctt ggcgagcttc gcgcagtcgt cgatacgtcc ggtcaacctg
      961 caggcacgcg catagacgcc accgaaccag aaccgcaggg gcgccgtctg gaaggacgtc
     1021 gacgagcgat cgagggcccg gtcggcgacg gccgtgagtt cgtcgcctcg gccaagcgca
     1081 ccgagcgcca tcaccaacgc gaacgacgcc atcatggcgt ggaagtcgcc gagttgctgc
     1141 gcgtcgagcg cggcggtggc cttctcggcg gccagttcgc agcggccgta caccgcgtcg
     1201 acgcacgctt caaccgcagc acgtgacgcg gcctgggtcg gcgtttccgg accggcggtt
     1261 agcccctcga ggatggccgc cgcatcgccg ggcctgccga gcgtccagat caggttggcc
     1321 gcccgcacgg tcgaccatcg gtgggcgtcc tcatggccgt cggcggtgat ctccttgagg
     1381 aacttctcgg cgtcctcgcc gcggccgcgc agcaacaggt tcatcgcctg cacgccgggc
     1441 gcttcaccgg ctcccgccga caccgccgcg caggcgaatc gatcggccag atcaagatcg
     1501 agcagcgtca tcgcgaagcg cgcggcgtcg acgaagaggt ccgggtcggg cgggaggtcc
     1561 gactcgagtg tgagcagggc gcgccgaacg gtggattgca tatcggcgtc gggcttcgcg
     1621 ccgaggcctt gcgccaactt gccgcgaatc ctcgacaggt acatctcgcc ggcgccggcc
     1681 cgtcggagtt ctccgtagag cgggtgcgcc aaccgggcca tgcgttcgtc gccggagcgc
     1741 tcgacggtga ccagccgcat ctgttcagcg gcttccaggt cggaacggtc ggccacggtg
     1801 cacagcacgt cgacggccag cggctcgcac tgcgacagcg tgtcgaccac gagcgccacg
     1861 ccttcgttca gctcacccag ttggcgaccg accatgtcgg tgatgttctg agacaccgca
     1921 acccggtcgt cccacatcca caccccggct acccgccgca tccgccctgc cgcaacctga
     1981 tcggtgacca gttgccgcaa gaacaaggcg ttaccgccgg tgagcttcca gaaccgttgc
     2041 gagctgcggg cgtccacagc tccgtccaaa gtggactcga tcaggctgcg cgttcctgcc
     2101 tccgaaagcg gctcgagatc gaggcgtgcc agcagcccgt ccttccacaa cgccgtcacg
     2161 gcgtcgggtt cggcctcccc cgtacgcatg gtgaccacaa gtcgcggacc acgtgtctgc
     2221 gcaagctgat gcacgacatg ggccgacaac ccgtcgagga ggtgcgcatc gtcgacgccg
     2281 atcagcacct tcccttgacg ttgttgagcg acaaaggaat tgatgagacg gccgacgtcg
     2341 ggcatcgtcc ctgaggcggt ttcggcaata gatccggtga acgcacccaa cggcagcggg
     2401 cgggcagatt cggttccggt gatccaattg gtccgctcac cggcggcttg ggcgcgcgcc
     2461 agtacctcgc gcgcgaggcg ggtcttgccg acacccgccg ccccgcagat cacgacgccg
     2521 gagtagttcc ccgtcccgct cagcgcacga cgaatcgcgc caagttcgct gtcgcgaccc
     2581 gtcagcagat cgctggtcat ttcaacgcct ccgcaccggc catgcgcccc tccgccggtc
     2641 atgaactgtt tta
//
LOCUS       DASYLX010000601         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1567043,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000601 DASYLX010000000
VERSION     DASYLX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567043"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2847)
                     /locus_tag="VGP27_14355"
     CDS             complement(88..2847)
                     /locus_tag="VGP27_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7852513.1"
                     /translation="MTARVVSREPQASEIADFLTSVVAAPSALVMEGEPGIGKTALWL
                     EAIDRARADGFHVMAARPVAAESVLAYASLADMLNGVDPAIMAQLPQPQRVALDRVLL
                     RAGGEHFPADQRAVAAGFLSVVCILAEITKVIVAVDDLQWLDSSSRLVLAFVARRLTG
                     RVGVLATVRTEDNGNAGSWLQLPRPDRVRRITVPPMSVGALHAVITNRLGHSYSRPTM
                     VRIEEASRGNPFYALEIARAVQGRQSNTEMSLPDSLLELIRSKIRGGDADVDDALLAV
                     ACLATPSVRLVARALDSDAEGVALRIEDAESRGIIDINGDQLRFSHPIFARGVYNDAA
                     PDRRRAMHRRLADVVDEPELRARHMALGSTTADAATLESLDSAAESARLRGAPAAAAE
                     LIELAIGLGGRTPHRLIQAAAHHFHAGESSRARALLEAAIAQLGPGVVRAEAMSWLGY
                     VRLLDDSFPEAADLLEEALAEVPDDPTVLIPTLVTLSFAQFNSGRLDASLQRVQEAVE
                     KAEQLGRPDLLSQALGMREMVRFLNGDGLDEPAMRRALQLEDQEGTIPLALRPTVHNA
                     IIRSCTGELDLAHKELTSLRRRCVDHGQDGELMLVAFHGTLVEIWRGHLAEAALIAED
                     TMQLAEQSGGDLPLSVALTCRALLCAYLGRVDEAHTDIEEARAASRRCGSDRLGEWPT
                     TALGFLEVSRGNYEAALGALLPLLAKVELAPKATEIIAASFIPDAVEAMVALGRLEPA
                     EQLVTTLERNGSRLQRNWMLAVGRRGRGMLLGARGDVPAAVEMVEQAMAAHRRLSMPF
                     ECARTQLLLGQLQRRKRLKDASAATIHDALATFEQLGTSLWADRARAELGRVDVGTLR
                     RVAMSPSEKRVAELAASGMTNRNIAAAMFISPKTVESNLSRIYVKLGIHSRAELGRHV
                     AARDV"
     gene            complement(2898..3929)
                     /locus_tag="VGP27_14360"
     CDS             complement(2898..3929)
                     /locus_tag="VGP27_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852514.1"
                     /translation="MRKALHPYVMAGVAVIGASALAVTPVIAAPPDIKIVNPEVRHTA
                     KAFDAYRDAVEHLLENLEALLGSALARPAPTDLSLELPRDSVFGDPAGSFPSLRNSRG
                     RDIDDPPLLLANMRARASDALEAALVDQAAGRIDLAVGQLLRESIYAVMQAFNSATMP
                     VAQSRSGLTVALAAALDSGEPGRVLNAIVTAPAPITGRVLYGDFGIGQVLRAAASSQA
                     DMSTTHSLRGESEAVVTLDLTAGARPAVVEDRHRVDDPDVVPDADEGTNSYVMADADG
                     EHRSDGNAHRPRLSGANSANERGGALRDGVRSGLHGIREGLRDAVKTLTGRGDDNGNN
                     TGVSGDESP"
     gene            complement(4545..4769)
                     /locus_tag="VGP27_14365"
     CDS             complement(4545..4769)
                     /locus_tag="VGP27_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEV7852515.1"
                     /translation="MPDRDPDTIKQDIDQARDQLAITVDTLAERANPRRIADDVKSGV
                     VRLVKKPAVAMSLAGLGVVLVALFVRRLRR"
BASE COUNT          878 a         1563 c         1531 g          837 t
ORIGIN      
        1 gataccactc gacgaggtag cagggcaccg gaatagtcat caattgaacg ctaaccaaga
       61 tggagcgcga aggaatcggg tgtttcccta tacatctcgc gcagcgacat gtcggccgag
      121 ctctgcgcgc gagtggatac cgagcttgac gtagatgcgg gaaaggttcg attcaactgt
      181 cttcgggctg atgaacatgg cggcggcgat gttgcggttg gtcatgcccg acgcagcgag
      241 ttcggccacc cgcttttcgg acgggctcat ggcaaccctt cgaagtgtgc cgacgtcgac
      301 tcggcccagc tcagcgcgcg cacggtcggc ccacagcgaa gtgcccagct gctcgaacgt
      361 cgccaacgca tcatgaatgg tcgccgccga ggcgtccttc aaacgcttac ggcgttgcag
      421 ctgacccagc agaagctggg tacgcgcgca ctcgaacggc atcgaaagcc ttcggtgtgc
      481 ggccatcgcc tgctcgacca tttcgaccgc cgcgggaacg tcaccccggg cgcccaacag
      541 catgcctcgg ccacgacgcc cgacggccag catccaattg cgctgtagcc gcgagccgtt
      601 gcgctcgagc gtcgtgacca gctgctctgc gggttcgagc cggcccaacg cgaccatcgc
      661 ctcgacggcg tcgggaatga atgacgcggc gatgatctcg gtcgccttcg gcgccaattc
      721 aaccttcgcc agcagaggaa gaagcgcgcc cagcgcggct tcatagttac ctcgcgacac
      781 ctcgaggaag ccgagcgccg tggtcggcca ttcgcccaat ctgtccgacc cgcaacggcg
      841 gcttgcggcg cgcgcttctt cgatatcggt atgcgcctcg tcgactcgac ccagataggc
      901 gcagagcagc gcacggcacg tcagcgccac cgaaagcggc aggtcaccgc ccgactgctc
      961 ggcgagctgc atcgtgtcct cggcgatcag cgctgcttcc gcgaggtggc cgcgccagat
     1021 ctcgaccagc gttccgtgaa aggcgacgag catcagctcg ccgtcctggc cgtgatcgac
     1081 gcatcgccgc cgaagcgacg tcagttcctt gtgcgccaaa tccaactcgc ccgtgcagga
     1141 ccgaatgatg gcgttgtgca cggtggggcg cagtgcgaga gggatggtcc cttcctgatc
     1201 ctcgagctgc agtgcccttc gcatcgccgg ctcgtcgagg ccgtcgccgt tgaggaagcg
     1261 gaccatctcc cgcataccga gtgcctgact caacaaatcc ggccgcccga gttgctcggc
     1321 cttctcgacc gcctcctgca cgcgttgtag cgacgcgtcg agtcgccccg agttgaactg
     1381 cgcgaaagac aatgtgacca gcgtagggat gagcactgtc ggatcatctg gtacttcggc
     1441 gagtgcttct tcgagcagat cggccgcctc ggggaagctg tcgtcgagca gccggacata
     1501 gccaagccaa ctcatcgctt ctgcccggac cacgccgggc ccgagttgcg caatcgctgc
     1561 ttcgagaagc gcgcgggccc ggctggattc gccggcatgg aagtggtgag ccgccgcttg
     1621 gatgaggcga tgtggcgtgc ggccaccgag gccgatcgcc agctcgatca gttcggccgc
     1681 ggcggccgga gccccgcgca ggcgggccga ctccgccgcc gagtcgagcg actcgagcgt
     1741 ggccgcgtct gccgtggtag atcccagcgc catgtgtctg gctcgcaatt ccggttcgtc
     1801 aacgacgtcg gccagtcgcc tgtgcatggc cctgcgccgg tcaggggcag cgtcgttgta
     1861 gaccccacgg gcgaagatcg gatggctgaa ccggagctga tcgccgttga tgtcgatgat
     1921 tccgcgactt tcggcgtcct cgatccgtag cgccacacct tcggcatccg agtccagagc
     1981 acgggcgacc agcctgaccg acggcgtggc cagacaggcc acggcaagca acgcatcatc
     2041 gacgtcagcg tcgccaccac ggatcttcga tcgaatcaac tccagaaggc tgtctggtaa
     2101 cgacatctct gtgttcgatt gacgtccctg cacagccctg gcgatttcca gcgcatagaa
     2161 cggattgccg cgggacgcct cttcgatccg caccatcgtt gggcgggaat acgagtgacc
     2221 cagtcggttc gtgattactg cgtgtagtgc gccgacgctc atgggaggca cggtgattcg
     2281 ccggacgcgg tcgggacgtg gcagctgcaa ccacgagcct gcattgccgt tgtcttcggt
     2341 gcggacggta gccagcacac cgacccgtcc ggtcagccga cgcgccacga acgcgaggac
     2401 gagacggctc gaggagtcga gccactgcaa atcatcaacc gccacaatga ctttcgtgat
     2461 ctctgcgaga atgcatacca cagagagaaa tccggcagcc accgcacgct ggtcggctgg
     2521 gaagtgttcg ccgcccgcac ggaggagcac gcggtcgagc gccacccgct gaggctgggg
     2581 caattgcgcc atgatggccg ggtcgacgcc gttcagcatg tcggccaagg aggcgtaggc
     2641 gagcaccgac tccgcggcga ccggccgcgc ggccatcacg tgaaagccgt cggcgcgggc
     2701 gcggtcaatc gcctcgagcc agagcgcggt cttgccgatg ccgggttcgc cttccatcac
     2761 caacgccgag ggcgcagcaa caaccgaggt caggaagtcg gcaatctcgg aggcctgcgg
     2821 ctcacggctg acgacccgtg ctgtcatgga ttcatgctga caggtggcaa ccctcgggtc
     2881 tatgcctcag ctacctatca cggagattcg tcgccgctga cgccggtgtt gttcccgttg
     2941 tcgtcaccgc ggccggtgag cgtcttgaca gcgtcgcgga ggccctcgcg gatcccgtgc
     3001 aacccgctgc ggacgccgtc acgcagggca ccgccgcgtt cgttggcgga gttcgcgccg
     3061 gacagccggg gacgatgcgc attgccatcg gatcgatgct cgccgtctgc atcggccatc
     3121 acgtaggagt tggtgccctc gtcggcgtct ggaacaacat ccggatcgtc gacacggtgc
     3181 ctgtcctcga cgaccgcagg gcgcgcgccc gcggtgaggt ccagcgtcac gaccgcctcg
     3241 gactcgccac gtagcgagtg ggtcgtcgac atgtcggctt gtgaggaagc ggccgcgcga
     3301 aggacctggc cgattccgaa atccccatag agcacgcggc ctgtgatcgg cgccggagca
     3361 gtgacgatgg cattcaggac tcgacccggt tcgccggagt ccagtgccgc ggcgagtgcc
     3421 acggttaagc cggaccggga ctgtgcgacc ggcatcgtcg ccgagttgaa cgcctgcatg
     3481 accgcataga tgctctctct gagcagttga ccgaccgcca gatcgatgcg gccggccgct
     3541 tggtcgacga gcgccgcctc cagcgcgtca gacgctctgg cccgcatgtt tgcgagcagg
     3601 agcggcgggt cgtcgatgtc gcgcccgcgc gagttgcgaa ggcttgggaa actacccgcc
     3661 ggatccccga aaacactgtc tcgcggcagt tcgagactca agtcagtcgg cgccggtctg
     3721 gccagcgccg aacccagcag ggcctctaga ttctccagga ggtgttcgac cgcgtctctg
     3781 taggcgtcga aggcctttgc cgtgtgacgg acttccggat tgacgatctt gatgtcgggc
     3841 ggcgcggcga taacgggagt gacggcaagg gcactggctc cgatcacggc aacaccggcc
     3901 atcacgtagg gatgaagagc cttgcgcatc gcgggtcccc ttcaagcgtt gtgaccacga
     3961 cgaacccaaa cctacctggg aatcggctgg caaacttcca attaagggat ccaccgctgt
     4021 tagccataat gcggcacgtt cggcgccatg cagcgggaga cacttgccac ctacgacatt
     4081 gccattgacc gatggttgcc gagatttgca gtccaacgac ctgcccggaa aaagaaacat
     4141 aaccgttaat atgtgctacc cgtcactgag gtcaagctac gctatccaga tcgcgaactg
     4201 tcactaggta acgggcgcgt atcagccatc cgacattcac cacgtcaagc tgcgtaaaag
     4261 cactgccctg tgttagtctt ccgttagctg ggggccgctg cgaggacaat ggctcacggc
     4321 ttgagggaag gaagggaggc ttagctgatg ctagcgttcg acgcgaagcg cacaccagat
     4381 tcgtcatccc gccctgccga aaatctcgtt gctgcgcagg tcaccgaggt gcacgagccc
     4441 gcagtcgtac ataactgcag ccggcttggc cgcagtgcga atccgccggg attctcgttc
     4501 tccgactggc tacgcgatcg gcgtagtcgc tgagagcgac ctgatcaacg cctgagcctg
     4561 cgcacgaaaa gcgccaccag cacgacaccc aaccccgcca gcgacatcgc cacggcgggt
     4621 tttttgacca acctgaccac tcccgacttc acgtcgtcgg cgattctgcg cggattggcc
     4681 cgttctgcga gcgtatcgac ggtgatggcc aactggtcac gagcctgatc gatgtcctgc
     4741 ttgatggtgt cgggatctcg atctggcact gtcgtcctcc atcgtcagcg tgggcgggag
     4801 cagttgtgc
//
LOCUS       DASYLX010000602         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_358295,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000602 DASYLX010000000
VERSION     DASYLX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358295"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VGP27_14370"
     CDS             complement(<1..1172)
                     /locus_tag="VGP27_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEV7852516.1"
                     /translation="MGMRPAAKMPKLTRRSRILITVALAVVLLLLIGPRLVDGYVNWL
                     WFGEMGYRSVFATQLLTKLVVFLVVWALVGAIVFGALALAYRTRPVFVPTTGPNDPVA
                     RYRTAVMTRLKLVGIGVPAVIGFFAGIVAQTYWERIQLFLHGGDFGVKDPQFGMDLGF
                     YAFDLPFYRLVISYLFVATFLALIANLLSHYLFGGVRLSGRTGALSRSARIQLISLVG
                     TLILLKAIAYWFDRYALLSNTRSGKPFTGAGYTDINAVLPAKLILMAIALICAVAVFS
                     AVVLRDLRIPAIGVVLLLLSSLVVGAGWPLVVEQISVKPNAAQKESEYISRSIKATRD
                     AYGLTDKTVTYRDYPGNAPATAQQVAADRATTSNIRLLDPTIVSPAFTQFQQGKNF"
     gene            complement(1251..2273)
                     /locus_tag="VGP27_14375"
     CDS             complement(1251..2273)
                     /locus_tag="VGP27_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV7852517.1"
                     /translation="MNRRISTLLVAMAPIVAFGVLLALVTVPYVSLGPGPTFDTLGEI
                     DGKEVVDIKGADVHPTTGHLNMTTVSQRDNLTLGQALTLWASGREQLVPRDLVYPPDK
                     SKDEIDKSNNQEFKRSEDSAEYAALGYLKYPPAVTAETVSKDGASAGKLKEGDAIDAV
                     NGTLVTDLEQFQGLMAKTKPGDEVVLDFRRKNAAPGVTTVKLGSAPDKKQGVLGIGVV
                     EAPWAPFTINFNLANIGGPSAGLVFSLAVIDKLTTGDLSGDRFVAGTGTINSDGKVGS
                     IGGITHKILAAHEAGATVFLVPADNCDEAKSAHQDGIDLVKVNKLGDAVDALKALSAG
                     GEASRC"
     gene            2367..>3304
                     /locus_tag="VGP27_14380"
     CDS             2367..>3304
                     /locus_tag="VGP27_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV7852518.1"
                     /translation="MADLPFGFSPGDDPERDKRNKDAESGSGSGSGQDPFGGGEFDIS
                     QLGQIFSRLGEMFSGAGSMTPGGKQSGPVNYDLARQLASSSIGFVAPVPEATSAAIGD
                     AVRLAETWLDGVTALPAGTTKAVAWTPTDWIDNTLDTWKRLCDPVAEQISTVWASALP
                     EEARAMAGPLLSMMTQMGGMAFGSQLGQALGKLSKEVLTSTDIGLPLGPKGLAALMPG
                     AIESLSEGLEQPRSEIMTFLAAREAAHHRLFSHVPWLPSQLLNAVEAFAKGMKIDMSG
                     IEEFAAGFNPASLTDPSQMEQLLNQGIFEPKATPEQT"
BASE COUNT          600 a         1099 c         1055 g          550 t
ORIGIN      
        1 tagaagttct tgccctgctg gaactgcgtg aaggccgggc tgacgatcgt cgggtccaac
       61 aaccggatgt tcgacgtcgt cgcgcggtcg gccgccacct gctgggcggt ggcaggcgca
      121 ttgcccggat agtcgcggta ggtgaccgtc ttgtcggtga gcccgtaggc atccctggtc
      181 gccttgatac ttcggctgat gtactcgctt tccttctgcg ccgcattggg tttgacgctg
      241 atctgttcga cgaccagcgg ccagcccgcg ccgacgacaa gcgacgacag cagcagcagc
      301 accacaccga tcgcgggaat ccgcaagtcg cgcaacacca ccgccgagaa caccgccacc
      361 gcgcagatca gcgcgatggc catcaggatg agcttggcgg gcagcaccgc gttgatgtcg
      421 gtgtagccgg cacccgtgaa cggtttaccg ctgcgcgtgt tcgacaacag cgcgtagcga
      481 tcgaaccaat aggcgatcgc cttgagcaat atcagcgtgc cgaccaggct gatcaactgg
      541 atacgcgccg accggctcaa cgcacccgtg cgccccgaca gccggacacc gccgaacagg
      601 tagtggctca acaggttcgc gatcaacgca aggaacgtcg cgacgaacag gtagctgatc
      661 accaaccggt agaacggcag gtcgaatgcg tagaacccga gatccatgcc gaactgcgga
      721 tccttgacgc cgaagtcgcc accgtgcagg aacagctgaa tgcgctccca gtacgtctgg
      781 gccacaatgc ccgcgaagaa gccgatgacc gcgggcacgc cgatgcccac cagcttcagc
      841 cgtgtcatca ccgcggtgcg gtaccgggcg acgggatcgt tgggaccggt agtcgggacg
      901 aacaccggcc gggtgcgata agcgagcgcc aatgccccga aaacgatggc accgaccagc
      961 gcccacacca ccaggaagac gacgagcttg gtgagcaact gcgtcgcgaa gacggaccga
     1021 tagcccatct cgccgaacca cagccagttg acatagccgt cgacgaggcg tggcccgatc
     1081 agcaacagca acacgaccgc cagggcgacc gtgatcagaa ttcggctacg tcgcgtcagc
     1141 ttcggcatct tcgccgcggg ccgcataccc actcgtcaac tcccgtgttc gcccaatgca
     1201 catctgattg gggtgcccca actctacgca ctgcgttcaa gccagcccgg tcaacaacgg
     1261 gatgcttcgc caccggcaga aagcgccttg agggcgtcca ccgcgtcgcc gagcttgttc
     1321 accttgacca ggtctatgcc gtcttgatgt gccgacttgg cctcatcgca gttgtcggca
     1381 gggacgagga acaccgtcgc gcctgcctcg tgcgcggcca ggatcttgtg ggtgatgccg
     1441 ccgatcgagc cgaccttgcc gtcggagttg atggtaccgg tgcccgcgac gaacctgtct
     1501 ccgctgaggt cgccggtggt cagcttgtcg atgacggcga ggctgaagac gaggcccgcg
     1561 gacggcccgc cgatgttcgc aaggttgaag ttgatggtga acggcgccca cggggcttcg
     1621 acgacgccga taccgagaac gccctgtttc ttgtccggcg cggagccgag ttttaccgtc
     1681 gtgacgcctg gggccgcgtt cttgcgccgg aagtcgagca ccacctcgtc gcccggcttg
     1741 gttttcgcca tcagcccctg aaactgctcc aggtcggtga ccaaggttcc gttcacggcg
     1801 tcgatggcat cgccctcttt gagcttgccc gccgacgccc cgtccttgct gaccgtctcg
     1861 gcagtcaccg cgggcgggta cttcaggtag ccgagcgccg cgtattcggc gctgtcctcg
     1921 gaccgtttga actcctggtt gttggacttg tcgatctcgt ccttggattt atcgggcggg
     1981 tagacgaggt cgcgcggcac caactgctcg cgacctgacg cccacagcgt cagcgcctgt
     2041 ccgagcgtca ggttgtcgcg ctgcgacacc gtggtcatgt tgaggtgtcc ggtcgtcgga
     2101 tgtacgtcgg cgcccttgat gtcgacgacc tctttcccgt cgatctcacc gagggtgtcg
     2161 aacgtcgggc cgggacccag cgagacgtac ggcaccgtca ccagagccag cagcacgccg
     2221 aaggcgacga tcggcgccat ggctaccagc agcgtagaaa tccgcctatt cacgccagca
     2281 acagtagacg gcctaccgaa cggggttcac tgagagcgtg acggcggccg cgacgcgacc
     2341 cgcccttggt gggtaccgtt gtggacatgg ctgacctgcc cttcggcttt tcgcccggag
     2401 acgaccccga gcgcgacaag cgcaacaagg acgccgaatc ggggtccggc tcgggctccg
     2461 ggcaggatcc gttcggcggc ggtgaattcg acatctccca actcggtcag atcttcagtc
     2521 gcctcggtga gatgttcagc ggcgccggca gcatgacacc cggcggcaag cagtccggcc
     2581 cggtcaacta cgacctcgcc cgccagttgg cgtcgagctc gatcggcttc gtcgcgcctg
     2641 tgccggaggc gaccagcgca gccatcggcg atgccgtgcg cctggccgaa acctggctcg
     2701 acggggtgac cgcgctgccg gcgggcacca caaaggccgt cgcgtggacg ccgaccgact
     2761 ggatcgacaa cacgctcgac acctggaaac ggctgtgcga tccggtggcc gagcagatct
     2821 cgacggtgtg ggcctcggcg ctgccggagg aggccagggc catggcgggc ccgctgctgt
     2881 cgatgatgac gcagatgggc ggcatggcct tcggctcgca gctcggccag gcattgggca
     2941 agctgtccaa ggaagtcctg acgtccaccg acatcggtct gccgctcggc ccgaagggtc
     3001 tcgccgcgct gatgcccggt gccatcgaat cgctttccga aggcctcgag cagccgcgca
     3061 gcgagatcat gacgttcctc gccgcccgcg aagccgcaca ccatcgtctg ttcagccatg
     3121 tgccgtggct gcccagccag ctgctcaacg ccgtcgaagc cttcgccaag ggcatgaaga
     3181 tcgacatgag cgggatcgag gaattcgcgg ccgggttcaa cccggcgtcg ctgaccgacc
     3241 cgtcgcagat ggagcagctg ctcaaccagg gcatcttcga accgaaggcc acacccgagc
     3301 agac
//
LOCUS       DASYLX010000603         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2991855,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000603 DASYLX010000000
VERSION     DASYLX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991855"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VGP27_14385"
     CDS             complement(<1..1144)
                     /locus_tag="VGP27_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7852519.1"
                     /translation="MTAERVAERIAHPTLDYRAIDVDNHYYEPTDSFTRHLPKEFKRR
                     GVQMLSEGKRTFAIMGGVVNHFIPNPTFDPIIEPGCLDLLFRGEIPEGVDPASLMKVD
                     RLADHPEYQNRDARVKILDRQNLETAFMLPTFACGVEEALKHDIEATMASVHAFNQWL
                     DEDWGFDRPDHRFLAAPIISLADPEKAVEEVEFVLGRDAKIVCVRPAPVPGLVKPRSL
                     GDPLHDPVWARLAEAGVPVVFHLSDSGYLAIAALWGGKATFEGFGKKDPLDQVLLDDR
                     AIHDSMASMIVHQVFTRHPTLKVASIENGAYFVYRLIKRLKKAANTAPYHFKEDPVEQ
                     LRNNVWIAPYYEDDVKLLAETIGVDKILFGSDWPHGEGLADPMSFTA"
     gene            complement(1141..2001)
                     /locus_tag="VGP27_14390"
     CDS             complement(1141..2001)
                     /locus_tag="VGP27_14390"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7852520.1"
                     /translation="MRIHQDETAREEIRGVADDDPVLYQLRDSGVAVLTLNRPERMNG
                     WGGGLAAKFYALLDRAEADPNVRAIVVTGAGRAFCAGADMGDLSSIEKANTSGETDVG
                     TLVGERHPHFVATLRKPVVAAINGACAGIGLTQALMCDVRFAAAGAKFTTSFARRGLI
                     AEYGISWILPRVVGWGVAMDLLLSGRVFLAEEAAALGLVNDVVAPEELLPRTIAYAED
                     IAASCAPSSLAVIKQQVYADTMRNVFEASDHAEKLMGESMRRPDFIEGITAFFEKRTP
                     NFPPLEEVTR"
     gene            complement(2043..3050)
                     /locus_tag="VGP27_14395"
     CDS             complement(2043..3050)
                     /locus_tag="VGP27_14395"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7852521.1"
                     /translation="MATAREAREWAHTALRGIGNSLYTPFSGMDGDDIDWDAYRTLVR
                     YCVGELKHPMLWCTSGIAEFWSLTIDERKRLLEIAIEEGRRANPDVVVQACTATTAAK
                     DCLELTRHAQEVGADIVYIQTPMMEAHGGQGVLNVFRYIADRTDIALGMFNSPSSGYV
                     LTPAESARIYAEIPAVCATKEGAFRPASSRLLHELAANLTIWECDLTVYRAGWLRAGI
                     VGPAQLGTAGYLYETPQRPIISEYWDLVWNDKLVEAMDYAEKSGLDQFGIDMASWFTC
                     YPGRPDYFTHWGGAFKYAASVLGLPIGDYPHSRPPQAEFPAEGKAQVDAAYRRFGLVP
                     E"
     gene            complement(3122..>3431)
                     /locus_tag="VGP27_14400"
     CDS             complement(3122..>3431)
                     /locus_tag="VGP27_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852522.1"
                     /translation="MVTSISKNLSLALAPDEIMVNVISPGSILSESLAGWADSVGIDG
                     SDPYAVMAGIDKHFGHPAHLPRAGRPDEIGPVVAFLVSRRNSYMTGANINVDGGSDFT
                     "
BASE COUNT          565 a         1145 c         1099 g          622 t
ORIGIN      
        1 cggcggtgaa cgacatcgga tccgcgagcc cctcgccgtg cggccagtcc gagccgaaca
       61 ggatcttgtc cacgccgatc gtctcggcaa gcagcttcac gtcgtcctcg tagtacggcg
      121 cgatccacac gttgtttcgc agctgctcga ccgggtcttc cttgaagtgg tacggcgcgg
      181 tgttggcggc cttcttcaac cgcttgatca agcggtacac gaaatacgcc ccgttctcga
      241 tgctggccac cttgagcgtg gggtgccggg taaaaacctg gtggacgatc atcgaggcca
      301 tcgagtcgtg gatggcacgg tcgtcgagca gcacctgatc cagcggatcc ttcttgccga
      361 acccttcgaa cgtcgccttg ccgccccaaa gcgccgcgat ggcgagataa ccgctgtcgg
      421 aaaggtggaa gaccaccgga acgccggctt cggcgagccg ggcccatacc gggtcgtgca
      481 gcggatcacc gagcgaccgc ggcttgacca gacctggcac cggcgcgggt cgcacacaca
      541 cgatcttggc gtcccggccc agcacgaact cgacttcctc gacggccttt tcgggatcgg
      601 ccagcgagat gatcggcgca gccaggaacc ggtgatcggg ccggtcgaag ccccagtcct
      661 cgtcgagcca ctgattgaag gcgtgcaccg aggccatggt cgcctcgata tcgtgcttga
      721 gggcctcttc gaccccgcac gcgaacgtcg ggagcatgaa ggcggtctcg aggttctggc
      781 ggtcgaggat cttcacccgg gcatcgcggt tctggtattc gggatgatcg gccaggcggt
      841 cgaccttcat cagcgacgcc gggtcgacgc cctcggggat ctcgccgcgg aacagcagat
      901 cgagacatcc gggctcgatg atgggatcga acgtcgggtt cgggatgaag tggttcacga
      961 cgccacccat gatggcgaag gtgcgcttgc cctcgctgag catctgcacg ccgcggcgct
     1021 tgaactcctt gggcagatgc cgggtgaacg agtcggtcgg ctcgtagtag tggttgtcga
     1081 cgtcgatcgc cctgtagtcc agtgtcggat gcgcgatgcg ctccgctact cgctctgctg
     1141 tcatcgcgtg acctcctcca agggtggaaa gttcggtgtg cgcttttcga agaacgcggt
     1201 gatgccctcg atgaagtccg gacgtcgcat ggattcgccc atcagcttct cggcgtggtc
     1261 gctggcctcg aacacattgc gcatggtgtc tgcgtacacc tgttgcttga tcaccgccag
     1321 agaactcggt gcgcagctcg cggcgatgtc ctcggcgtag gcgatcgtcc ggggcagcaa
     1381 ctcttccggt gccacgacgt cgttgacgag gccaagcgcc gcggcctcct cggcaaggaa
     1441 cactcgaccg gacagaagca ggtccatcgc cacgccccag ccgacgacgc gcggaagtat
     1501 ccagctgatg ccgtactccg cgatcagacc gcgacgcgcg aacgaggtgg tgaatttcgc
     1561 tcccgcggca gcgaaccgga cgtcgcacat cagcgcctgg gtcagcccga tgcccgcgca
     1621 cgccccgttg atcgcggcaa cgaccggctt gcgcagcgtc gcaacgaaat gcggatgccg
     1681 ttcgccgacc aaggtgccga cgtcggtctc gccgctggtg ttggccttct cgatcgacga
     1741 cagatccccc atgtcggccc ctgcgcagaa cgctcgcccc gcgccggtga cgacgatcgc
     1801 ccgcacgttc gggtcggctt cggcacggtc gagcagcgcg tagaacttcg ctgcgagccc
     1861 gccgccccag ccgttcatcc gctcagggcg gttcaatgtc agcacagcca cgccgctgtc
     1921 ccgaagctgg tagaggaccg ggtcgtcgtc agccaccccg cggatctcct cacgtgcggt
     1981 ttcgtcctga tgtatgcgca tactgtacac ctatcggtag cgcgttccgc aacgggctgc
     2041 ggtcattcgg ggaccagtcc gaagcgccgg tacgcagcat cgacctgcgc cttcccctcg
     2101 gcggggaact cggcttgggg cggccgcgaa tgcgggtagt cgccgatcgg cagaccgagc
     2161 accgacgccg cgtatttgaa cgccccgccc cagtgcgtga agtagtccgg tcggccggga
     2221 tagcacgtga accaggaggc catatcgata ccgaactggt ctagtccgga cttctcggcg
     2281 tagtccatcg cctcgaccag cttgtcgttc cagacaagat cccaatactc ggagatgatc
     2341 ggccgctgcg gagtttcgta cagatagcct gccgtaccga gctgtgccgg accgacgatc
     2401 cccgcgcgca gccacccggc gcggtagacg gtcaggtcgc actcccagat cgtcaggttg
     2461 gccgcgagct cgtgcagcag cctgctgctc gccggccgga atgcgccctc cttcgtcgcg
     2521 cagacggcgg gaatctcggc atagatccgc gcactctccg ccggcgtcag cacgtagccc
     2581 gacgacggcg agttgaacat gcccagtgcg atgtcggtgc gatcggcgat gtagcggaag
     2641 acgttcagca ccccttgccc gccgtgggcc tccatcatcg gcgtctggat gtagacgatg
     2701 tcggccccga cctcctgggc gtgtcgcgtc agctcgagac agtccttggc ggccgtggtc
     2761 gcggtgcagg cctgcacgac gacgtcgggg ttggcccgtc ggccctcctc gattgcgatc
     2821 tccaacaggc gttttcgctc atcgatcgtc agcgaccaga attccgcgat gccgctggtg
     2881 caccacagca tcgggtgttt cagctcgccg acgcagtacc gcaccaacgt ccggtaggcg
     2941 tcccagtcga tgtcgtcgcc gtccattccg gagaacgggg tgtagaggct gttgccgatt
     3001 ccgcgcaacg cggtatgtgc ccactcgcgg gcctcgcgtg ctgttgccat gccgccccct
     3061 ttatctcatt gccgcgctcc tcacagcgac tcgtccctcg ccgcttgatc gtcgcgcggc
     3121 gctaggtgaa gtcgctgccg ccgtcgacgt tgatgttggc gccggtcatg taggagttgc
     3181 gccgcgacac cagaaacgcg acgacgggac cgatctcgtc gggccttccg gcgcgcggca
     3241 gatgcgcggg atgcccgaaa tgtttgtcga tgcctgccat cacggcgtac gggtcgctgc
     3301 cgtcgatgcc gaccgaatcg gcccaacccg ccagcgactc cgacaggatg ctgcccggcg
     3361 agatcacgtt caccatgatc tcgtcggggg ccagcgccag cgacaggttc ttggagatgc
     3421 tggtgaccat c
//
LOCUS       DASYLX010000604         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2662278,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000604 DASYLX010000000
VERSION     DASYLX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662278"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VGP27_14405"
     CDS             <1..1267
                     /locus_tag="VGP27_14405"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV7852523.1"
                     /translation="QVVRIIEESAGDLAARIGAPLVLRGVGVRRVADDRGVPVDMLTS
                     DIDELVSRDDVDIVVEVMGPVETARKAILSALEKGKSVVTANKALMAQSTGELAQAAE
                     HAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSEMDSTGAEYSA
                     ALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADF
                     ETARALGCTIKLLSICERLTNDEGQQRVSARVYPALVPLTHPLASVNGAFNAVVVEAE
                     AAGRLMFYGQGAGGAPTASAVMGDLVMAARNRVQGGRGPRESKYAKLPISPIGFIPTR
                     YYVSMNVADRPGVLASVAAEFGKREVSIAEVRQEGVVDEGGQRCGARIVVVTHEATDA
                     ALSETVAALTDLDVVQAVTSVLRLEGTSE"
     gene            1264..2349
                     /gene="thrC"
                     /locus_tag="VGP27_14410"
     CDS             1264..2349
                     /gene="thrC"
                     /locus_tag="VGP27_14410"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV7852524.1"
                     /translation="MSSTRAEPVHKPWPGLIAAYGDRLPVEDSWTPITLLEGGTPLIH
                     ATRLSERTGCTVHLKVEGLNPTGSFKDRGMTVAVTEALARGQKAVLCASTGNTSASAA
                     AYAARAGITCAVLIPQGKIAMGKLAQAVMHGAKILQIDGNFDDCLELARKITTDYPTV
                     ALVNSVNPYRIEGQKTAAFEIVDVLGTAPDVHALPVGNAGNITAYWKGYTEYHRDGLS
                     DRLPRMLGTQAAGAAPLVLGEPVKSPETIATAIRIGAPASWTSAVEAQQQSGGRFLAA
                     TDEEILTAYRLVAASEGVFVEPASAASIAGLLKSIEDGWVAKGSTVVCTVTGNGLKDP
                     DTALKDMPTVTPVPVDAGLVVAELGLV"
     gene            2362..>2841
                     /locus_tag="VGP27_14415"
     CDS             2362..>2841
                     /locus_tag="VGP27_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV7852525.1"
                     /translation="MTPTLPAGLTATSVVAASSANLGPGFDSLGLALSLYDEIVVETT
                     QSGLTVDVEGEGAGQVPLDSTHLVVRAIQHGLRAVGCAVAGLNVYCRNNIPQSRGLGS
                     SAAAVVGGLAAVNGLVSQAGSQPMSQAELIQRASEFEGHPDNAAAAVLGGGVVSWTST
                     "
BASE COUNT          471 a          953 c          975 g          442 t
ORIGIN      
        1 ccaggtggtc cgcatcatcg aggagagtgc cggggatctg gccgcacgca tcggtgcgcc
       61 actggtgctg cgcggcgtcg gcgtgcgcag ggtcgccgac gaccgtggcg tgccggtgga
      121 catgctgacc agcgacatcg atgagttggt ctcccgcgac gacgtcgaca tcgtcgtcga
      181 ggtgatgggc cctgtcgaaa ccgcccgcaa ggcgatcctg tcagcgctcg agaagggcaa
      241 gtcggtcgtc accgccaaca aggcgctgat ggcgcagtcc accggtgagt tggcccaggc
      301 cgccgagcac gcccacgtag acctgtactt cgaggctgcg gtcgccggtg ccatcccggt
      361 catccgtccg ctcacccagt ccctcgccgg agacaccgtg ctgcgggtgg caggcatcgt
      421 gaacggcacc accaactaca tcctttcgga gatggacagc accggcgccg agtattcggc
      481 cgcgctcgcc gacgcgagcg ccctcggtta tgccgaagcc gatcccaccg ccgacgtcga
      541 gggatacgat gcggcggcga aggctgccat cttggcttcc atcgccttcc acacccgggt
      601 caccgccgac gacgtctacc gcgagggcat caccaaggtg acgcccgccg acttcgagac
      661 cgcgcgcgcc ctcggatgca ccatcaagct cctgtccatc tgtgagcgac tcaccaacga
      721 cgaagggcaa cagcgggttt cggcccgtgt ctacccggcg ttggtgccgc tgacgcatcc
      781 cctggcatcc gtcaacggag cgttcaatgc ggtcgtcgtg gaggcggagg cggccggccg
      841 gctgatgttc tacggacagg gcgccggtgg agcgccgacg gcatcggccg tgatgggaga
      901 cctcgtgatg gccgcgcgca accgggtgca gggtggccgt ggtccccgcg agtccaagta
      961 cgccaagctt cccatctcac cgatcgggtt cattcccacc cgctactacg tgagcatgaa
     1021 cgtcgccgac cgtcctggcg tattggcctc tgtcgcagct gaattcggca agcgggaggt
     1081 cagtatcgcc gaagtcaggc aggagggcgt ggtcgatgaa ggcggccaac gctgcggtgc
     1141 gcggatcgtg gttgtcaccc atgaagccac cgacgcggcg ctgtcggaga ccgtcgcggc
     1201 actcaccgac ctcgacgtcg tgcaggcagt gaccagcgtg cttcgcctcg agggaacgag
     1261 cgaatgagtt cgacgcgcgc ggagccggtg cacaagccgt ggccgggtct gatcgcggcc
     1321 tacggtgacc ggctgccggt cgaggacagc tggacgccga tcacgctgct ggaaggcggt
     1381 acgccgctga tccacgccac gcggctgtcc gagcgcaccg gctgcacggt gcatttgaag
     1441 gtcgagggac tcaaccccac gggctcgttc aaggaccgcg ggatgacggt ggccgtcacc
     1501 gaagcgctgg cccgtggcca gaaggccgtt ctgtgcgcgt ccaccggcaa cacctcggcg
     1561 tcggcggcgg cctacgccgc gcgtgcgggc atcacctgtg cggtgctgat cccacagggc
     1621 aagatcgcga tgggcaagct cgcgcaggcc gtgatgcacg gggccaagat cctgcagatc
     1681 gacggcaact tcgacgactg cctggagttg gcgcgcaaga tcaccaccga ctatccgacg
     1741 gtcgcgctgg tcaactcggt caacccctac cgcatcgagg ggcagaagac ggcggccttc
     1801 gagatcgtcg acgtattggg caccgcaccc gatgtgcacg ccctgccggt cggcaacgcg
     1861 ggcaacatca ccgcgtactg gaagggctac accgagtacc accgcgacgg gttgtccgac
     1921 cgactgccgc ggatgctggg cacccaggcc gccggcgcgg cgccgctggt tctcggtgag
     1981 ccggtcaagt cgcccgagac catcgcaacc gcgattcgga tcggcgcgcc cgcatcctgg
     2041 acctcggccg tcgaggccca gcagcagtcg ggtggccgct tcctggctgc gaccgacgaa
     2101 gagatcctga ccgcctatcg cctggtcgcg gcaagcgaag gcgtgttcgt cgagccggcg
     2161 tcggcggcca gcatcgccgg cctgctcaag tccatcgagg acggctgggt ggccaagggg
     2221 tcgacagtgg tgtgcacggt gaccggcaac ggtctgaaag atcccgacac ggcgctcaag
     2281 gacatgccca cggtcacgcc ggtccccgtc gacgccggcc tcgtcgtcgc agagctcgga
     2341 ctggtctgac ggagatcgtc ggtgacgcca accctgccag ccggactgac agctaccagt
     2401 gtcgttgcgg catcgagtgc caatctcggg ccgggcttcg acagcctcgg actcgcgctg
     2461 agcctgtacg acgaaatcgt cgtggagaca acccaatcgg gcctcacggt cgacgtcgag
     2521 ggtgagggcg ccggccaggt gccgttggac tcgacgcacc tcgtcgtgcg agccatccaa
     2581 cacggcctgc gtgccgtcgg ctgcgctgtt gcggggctga atgtctactg ccgcaacaac
     2641 attccgcagt cgcgcggact cggctcatcg gcggccgcgg tggtcggcgg gctggcggca
     2701 gtcaacggtc ttgtcagcca agcgggttcg caaccgatgt cgcaagcgga gctgattcag
     2761 cgcgcatcgg agttcgaggg tcatccggac aacgccgctg ccgccgttct gggcggcggc
     2821 gtggtgtcgt ggacttcgac c
//
LOCUS       DASYLX010000605         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_140449,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000605 DASYLX010000000
VERSION     DASYLX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140449"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1415
                     /gene="mtr"
                     /locus_tag="VGP27_14420"
     CDS             3..1415
                     /gene="mtr"
                     /locus_tag="VGP27_14420"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050627 - mycothione reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HEV7852526.1"
                     /translation="MEHFDIAIIGTGSGNSLLDERYTDKRVAICEQGTFGGTCLNVGC
                     IPTKMFVYAAEVAQNARDTARFGVDATLDGVRWNDIVSRVFGRIDPIAMGGENYRRSS
                     PNVSVFDTHTRFAERTADGRYMLRTEGGQEFTADQVVIAAGSRVSVPDAVRDCGVDHY
                     TSDTIMRIGELPSHLVIIGGGFVAAEFAHTFAALGTRVSLVIRGGTLLKHCDDTICER
                     FTDIASKKWEIHNHCNVVGATQDGNGITLTMDNGATLQADAVLVATGRIPNGDQLDAE
                     SAGIEVRDGSIVVDEYQRTAARGVFALGDVSSEYQLKHVANHEARVVRENLLLDWDDT
                     ASLKRSDHRYVPSAVFTDPQIAMVGLTENQARALGYDVRSKVQDYGDVAYGWAMEDTT
                     GIAKIIVEAGTGKILGAHIMGYQASSLIQPIIQAMSFGLAGQDMARGQYWIHPALPEV
                     IENALLSLCGEPPWPPARRH"
     gene            complement(1417..2514)
                     /locus_tag="VGP27_14425"
     CDS             complement(1417..2514)
                     /locus_tag="VGP27_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEV7852527.1"
                     /translation="MSIATQSKVSDSKRFDRTDWITIASIGGVVLALHVFGWGVLIFG
                     VAPQHITLGSAGVFGVGLGVTAYLLGVRHAFDADHIAVIDNTTRKLVGEGTRSLSAGF
                     WFSLGHSSVVFGLAFLLAIGVRAVVGPVQDEGSPLLQTLGLVGSLVAGTFLILIGLSN
                     LFAAVGIAKVFRRMRSGELDEAELERQLQQRGLLARLFARVMRRVSKPGHLYPVGLLM
                     GLGFDTATQVALLVLAAGTAAFTLPWYAILVLPVLFAAGMSLFDAIDGILMSRAYGWA
                     FLQPVRKVYYNLTVTVLSVIVALVIGVIVLAGLLADRLGVHTGPLAVIGSANLQFVGF
                     MIVGLFVAAWLIALAVWRFGEVEKRWAPAHD"
     gene            complement(2600..2929)
                     /locus_tag="VGP27_14430"
     CDS             complement(2600..2929)
                     /locus_tag="VGP27_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7852528.1"
                     /translation="MDKPVKQLLVERASSALADVDTAGWTLRFDLLSDPNRLEILLCL
                     HRAPGICVGDLGAALGRSENAVSQALRVLRQQGWVSSTRVGREVSYRLEDEIVHELLH
                     RIGAQHG"
BASE COUNT          602 a         1033 c          929 g          444 t
ORIGIN      
        1 ggatggaaca tttcgacatc gcaatcatcg gcaccggctc gggcaattct cttctcgacg
       61 agcgctacac cgacaagcga gtggcgatct gcgaacaggg cacgttcggc ggaacgtgtc
      121 tcaacgtggg ctgcatcccg acgaagatgt tcgtctatgc cgccgaggtg gcgcagaacg
      181 caagggacac agcgcgcttc ggtgtcgacg cgacgctcga cggcgtccgc tggaacgaca
      241 tcgtctcgcg cgtgttcggt cgcatcgacc cgatcgcgat gggtggcgag aactaccgcc
      301 gctcgtcgcc gaacgtctcg gtgttcgaca cccacacccg cttcgccgag cgcaccgccg
      361 acggccgcta catgttgcgc accgaaggcg gccaggagtt caccgccgac caggtggtga
      421 tcgccgcggg ctcgcgtgtg tctgtgcccg acgcggtccg cgactgcggt gtcgaccact
      481 acaccagcga caccatcatg cggatcggtg aactgccatc ccatctcgtc atcatcggcg
      541 gcggcttcgt cgccgcagaa ttcgcacaca ccttcgcggc gctgggtacc cgggtctcgc
      601 tcgtcatccg cggcggcacg ctgctcaagc actgcgacga caccatatgc gaacggttca
      661 ccgacatcgc gtccaagaag tgggagatcc acaaccactg caatgtggtc ggcgcgacgc
      721 aggacggcaa cggcatcacc ctcaccatgg acaacggcgc gacgctacag gccgacgcgg
      781 tgctggtggc gaccggccgg atcccgaacg gcgaccagct cgacgccgaa tcggccggaa
      841 tcgaggttcg cgacggaagc atcgtcgtcg acgagtacca acgcaccgcg gcccgtggtg
      901 ttttcgcgct cggtgacgtc agctctgagt accagctcaa gcatgtggcc aaccatgagg
      961 cccgcgtggt gcgggagaac ctgctgctgg actgggacga caccgcgtcc ctcaaacggt
     1021 ccgaccaccg ctacgtgccc tcggccgtct tcaccgaccc gcagatcgcg atggtaggcc
     1081 tgaccgaaaa ccaggcccgc gcactgggat acgacgtcag atcgaaagtg caggactacg
     1141 gtgacgtggc ctacggctgg gcgatggagg acaccaccgg catcgccaag atcatcgtcg
     1201 aagccggaac cggcaagatc ctcggcgcac acatcatggg ctatcaggcc tcatcgctga
     1261 ttcagccgat catccaggcg atgagtttcg gactcgccgg ccaggacatg gcccgcggcc
     1321 agtactggat tcacccggcg ctgccggagg tcatcgagaa cgcgctgctt tcgttgtgcg
     1381 gcgagccgcc atggccgccc gcccgcaggc actgacctag tcgtgcgcag gagcccagcg
     1441 cttctcgacc tctccgaagc gccaaaccgc cagggcgatc aaccaggccg cgacgaacag
     1501 tccgacgatc atgaacccga cgaactgtag gttcgccgat ccgatcacgg ccagcggacc
     1561 cgtatgcacg cccaggcggt cggccagaag gccggcgagc acgatgacgc cgatgaccag
     1621 agccacgatc accgacaaca ccgtcacggt caggttgtaa taaaccttgc ggacaggctg
     1681 aaggaaggcc cacccgtagg cccgcgacat caggatcccg tcaatcgcat cgaagaggct
     1741 catcccggcg gcgaacaaca ccggcagcac caggatggcg taccaaggca gggtgaaggc
     1801 tgccgttccc gcggcgagga ccagcaatgc cacctgagtg gccgtgtcga agccgaggcc
     1861 catcagcagc ccgaccgggt agagatggcc tggcttactc acccggcgca tgaccctggc
     1921 gaacagccgc gccagaagtc cacgctgctg cagctgccgc tcgagttcgg cctcatcgag
     1981 ctcgccgctg cgcattcggc ggaacacctt ggcgatgccc accgcggcaa acaggttcga
     2041 gagcccgatc agaatgagga aggtgcctgc gaccagcgag ccgaccaacc cgagggtctg
     2101 cagcagcggg gaaccctcgt cctgcacggg tcccaccacg gcgcgcacgc cgatcgccaa
     2161 caggaacgcc aggccgaaca cgacgctcga atggccgagt gagaaccaga agccggcgga
     2221 cagcgatcgc gttccctccc cgaccagctt gcgggtggtg ttgtcgatga cggcgatgtg
     2281 gtcggcgtcg aacgcgtgcc gcaccccaag cagataggcc gtcacgccga gcccgacacc
     2341 gaatacaccc gcggacccga gggtgatgtg ctgcggcgcg acaccgaaga tcagaacgcc
     2401 ccaaccgaag acgtgaaggg ccagcacgac accaccgatc gaggcgatcg tgatccaatc
     2461 ggttcgatcg aatcgcttgg aatcggagac cttcgactgc gtcgcgatgc tcaccagccg
     2521 agcctaggac ccaagctgca tacctgtcta gctgaacaga tcttcaggta tgtctcagtc
     2581 cggcgcgagc aaccggctct cagccgtgct gggcaccgat gcggtgtaac agctcgtgga
     2641 cgatttcgtc ctcgagccgg tagctgacct ctcggccgac ccgggtggaa ctcacccagc
     2701 cctgctgccg cagcacccga agcgcttggg acacagcgtt ttcagaccgc ccgagcgcgg
     2761 cgcccagatc ccctacgcag atccccggcg ccctgtgcag gcacagcagg atctccagcc
     2821 ggttgggatc ggacagcaga tcgaagcgca aggtccagcc cgcggtgtcg acgtcggcca
     2881 gcgccgacga cgcgcgctcg accagaagct gcttcacggg cttgtccact gcgatcaatg
     2941 taatccagcc gccggtgtca gcggcccaat cacccgcagt cgtttcccgc taccgatcgc
     3001 ttttcgtc
//
LOCUS       DASYLX010000606         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3650472,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000606 DASYLX010000000
VERSION     DASYLX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3650472"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGP27_14435"
     CDS             complement(<1..303)
                     /locus_tag="VGP27_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV7852529.1"
                     /translation="MSRPDYQAPGWHNVRRMPATVLQALQDDGTYPDLYYGKNLLDEV
                     PQDLYKQDWWYRTTFTAPAGHTTYLLELPGINYRAEVWLNGRLVADNKQIVGMFSAH"
     gene            561..1277
                     /locus_tag="VGP27_14440"
     CDS             561..1277
                     /locus_tag="VGP27_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEV7852530.1"
                     /translation="MIVEPSGRIAEIAASSPGPEVGAFFDLDGTLVSGFTATAHAGDR
                     IRRRQARIGEVLGVLEASIRYRLGRMQFERLIMRAAGYLKGESLRELDELGEALFVRR
                     IASRVYPHMREVVAAHRECGHTVALCSSALTIHAEPVARALGITRLLCNHFEVDGNGL
                     LTGDIERPIIWGARKVDAAQRFCEDQGVDMQRSYFYADGDEDVPLMSVVGHPRPVNPR
                     PGLAAAAAANGWPVLRVAVR"
     gene            1274..1735
                     /locus_tag="VGP27_14445"
     CDS             1274..1735
                     /locus_tag="VGP27_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852531.1"
                     /translation="MSVSEDLLLACSGFLLAVLWMDLIFDTQARGARNARLSDPVLAS
                     ISAYYRRATTTSRPMSRLIAAVMVVLLAALAFRAVRGHDPAWLAAASAVLAGAPILLA
                     GIRTVRNAVRLGGRGDGAAEQSRLARSLYRDHLVCFACMVAFLVFRLVAVW"
     gene            1828..2160
                     /locus_tag="VGP27_14450"
     CDS             1828..2160
                     /locus_tag="VGP27_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV7852532.1"
                     /translation="MNARRLILLVGAALLVAGVIALLVPVSITGNGKSIGCGNAVAAD
                     SSQAEAANGQTVADVPILNQIVPHTDYVAECNHAVSSRRSWAIPLAVVGLLVAAGSLV
                     VPRTRTVS"
     gene            2225..2713
                     /locus_tag="VGP27_14455"
     CDS             2225..2713
                     /locus_tag="VGP27_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852533.1"
                     /translation="MANEDQQTQDKDSEQQNDRPLTEKPEVKDEHKEKAQEMRKEYVE
                     ERATVIMPGSGGTVAGTAVNEWLDDDGNPKFGEESESEGGKSDGDDSDRGGSDDDKSL
                     GKRLEEDKARNERIVKAHDEAANDEAQNDEAGNDQARDDEAEDDEAADDGAQRDEAKN
                     DG"
     gene            2793..>3599
                     /locus_tag="VGP27_14460"
     CDS             2793..>3599
                     /locus_tag="VGP27_14460"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7852534.1"
                     /translation="MHTAICDDLGIEFPIFAFTHCRDVVVAVSKAGGFGVLGAVGFTP
                     EQLEIELKWIDENIGDHPYGVDIVIPNKYEGMDSTDLEPEVLKKQLNALVPDEHLDFA
                     RKILADHGVPVDHSDDDALQLLGWTEATATPQVEVALKHPKVTLIANALGTPPPDMIK
                     HIHDEGRKVGALCGSPSQARKHADAGVDIIIAQGGEAGGHCGEVGSIVLWPQVVKEVA
                     PIPVLAAGGIGSGQQIAAALALGAQGAWTGSQWVMVEESEHTPVQHAAYAK"
BASE COUNT          642 a         1099 c         1269 g          589 t
ORIGIN      
        1 atgtgcgctg aacatgccga cgatctgttt gttgtcggcg accaggcggc cgttgagcca
       61 gacctcggcg cggtagttga taccgggcaa ctcgagcaga taggtggtgt gacccgccgg
      121 cgccgtgaac gtcgtgcggt accaccagtc ctgcttgtag aggtcctgcg gaacctcgtc
      181 gagcaggttc ttgccgtagt acaggtcggg gtaggtgccg tcgtcctgca acgcctgcag
      241 gacggtcgcg ggcatgcggc ggacgttgtg ccaacccggc gcctggtagt cgggacgcga
      301 caccgtcgcg ccgtcgctgc ccagtccggt ggccgacgcg agctgccagc cgtcagcgag
      361 ttcgactcgc tcgggcggct gctgagggcg cgcgaaggtc ggcagcgaac cggtgcagac
      421 caggagtaac aggacgatga ttacgacgga gagtgtcttg cgacgccggg gcgatatggc
      481 actctccttt cggtttgagt cgacactacg gttgatgacg caatccggct gactgtgatc
      541 aagaaccgta gtctcgggac atgatcgtgg agccgtcggg gcggatcgcc gagatcgccg
      601 caagctcgcc gggccccgag gtcggcgcgt tcttcgactt ggacggcacc ttggtttccg
      661 ggttcacggc gacggcccac gccggcgatc gaatccggcg caggcaagcg cggatcggcg
      721 aggttctcgg ggtcctggaa gcctcgatcc ggtaccggct cggccgcatg cagttcgagc
      781 ggctcatcat gcgggcggcg gggtatctga agggcgagtc gctgcgcgaa ctagacgagc
      841 tgggcgaggc gctgttcgtc cgacgcatcg cgtcgcgggt gtacccgcac atgcgtgagg
      901 tggtggcggc ccaccgagag tgcggccata ctgtcgcgct gtgctcgtcg gcgctgacga
      961 tccatgccga accggtggcc cgcgcactgg gaatcacccg gctgctctgc aatcacttcg
     1021 aagtcgacgg caacggcctg ctgaccggcg acatcgagag gccgatcatc tggggtgcca
     1081 ggaaagtgga tgcggcacag cgcttctgcg aagaccaggg cgtggacatg caacgcagtt
     1141 atttctacgc cgacggtgac gaagacgtgc cgctgatgtc ggtggtcggc catccccgac
     1201 cggtgaaccc gcgcccgggc ctcgctgctg cggcggcggc caacggctgg ccggtgctgc
     1261 gcgtcgccgt gcgatgagcg tttcagaaga ccttcttctc gcctgtagcg gcttcctgct
     1321 cgcagtgttg tggatggacc tgatcttcga cacccaagcc cgcggcgccc ggaatgcgcg
     1381 gttgtccgac ccggtgctcg cgtcgatctc ggcctactac cggcgcgcga cgacgacgtc
     1441 gcgtccgatg agccgcctga tcgccgcggt gatggtcgtt cttctcgcag cgttggcctt
     1501 tcgcgccgtg cgcggacacg accccgcctg gctcgccgcc gcttcggctg tgctcgccgg
     1561 ggcgcccatc ctgctcgccg ggatcagaac ggtgcggaac gcggttcggc tcggcggtcg
     1621 aggcgacggg gcggccgagc agtcgcggct cgcgcggtcg ctttatcggg atcacctcgt
     1681 gtgcttcgcg tgcatggtcg cgtttctggt gttccggctg gttgctgttt ggtgagcacg
     1741 tcggcgggca atccctgacc gggctcggga acgccgagtt caccacttgg ccgtgtcttc
     1801 ccggccgcag agacgaggaa cctgactgtg aatgcacgac gcttgatcct gctggttggt
     1861 gcggcactcc tggtcgccgg tgtgatcgcc ttgttggttc cggtatcgat caccggaaac
     1921 ggcaagtcga tcggctgcgg caatgcggtg gccgcagatt cgtctcaggc cgaggccgcc
     1981 aacggccaga ccgttgcaga cgtgccgatt ctcaaccaga tcgttccgca caccgactac
     2041 gtcgccgagt gcaaccacgc cgtgtcgagc aggcggtcct gggccattcc gctggccgtg
     2101 gtgggcctgc tggtggcggc cggctccctg gtcgtgccga gaacccggac tgtttcgtaa
     2161 atcacacttt cgggcagtcc tagaagcacg aatccgctag agacctttag accgagggga
     2221 cgccgtggcg aacgaagatc aacagacgca agacaaggac agcgagcagc agaatgatcg
     2281 gccgctgacc gagaagcccg aggtcaaaga cgagcacaaa gagaaggccc aggaaatgcg
     2341 gaaggagtac gtggaggaac gtgccacggt gatcatgcca ggcagcggtg gcaccgtggc
     2401 ggggacggcc gtcaacgagt ggctcgatga cgatgggaac ccgaagttcg gggaggaatc
     2461 ggagtcagag ggcggcaagt cggatggcga cgactcggac cgcggcggct cagatgacga
     2521 caagtcactt ggcaagaggt tggaggagga caaggcaaga aacgagagga tcgtcaaggc
     2581 acacgacgag gcagccaacg acgaggcaca gaacgacgag gcgggaaacg accaggctcg
     2641 ggacgacgag gcagaggatg acgaggcagc ggacgacgga gcacagaggg acgaggcgaa
     2701 gaacgacggt taggcgaccc cgtagtggtc gcggcgccgg tgcgtactaa actagaacac
     2761 gtttcagttc gcccactgcc ctaggagccg gcatgcacac ggccatctgc gacgacctcg
     2821 gcatcgaatt tccgatcttc gccttcaccc attgccgtga cgtggtcgtc gcggtcagca
     2881 aggcgggtgg tttcggcgtc ctcggtgccg tcggcttcac ccccgagcag ctcgagatcg
     2941 aactcaagtg gatcgacgag aacatcggcg atcaccctta cggcgtggac atcgtcattc
     3001 ccaacaagta cgagggtatg gattcgacag atctcgagcc tgaagtgctc aagaaacaac
     3061 tcaacgccct cgtgccggac gagcacctgg acttcgcgcg caagattctg gcggaccacg
     3121 gcgtgccggt cgatcacagc gatgacgacg cgctgcaact cctcggttgg accgaggcca
     3181 ccgcgacgcc gcaggtcgag gtggcgctga agcatccgaa ggtgactttg atcgcgaacg
     3241 ccctcggcac gccgccgccc gacatgatca agcacatcca cgacgaaggc cggaaggtcg
     3301 gcgcgctgtg cggatcgccg tcgcaggcgc gcaagcacgc cgacgcgggc gtcgacatca
     3361 tcatcgccca gggcggcgag gcgggcggac actgcggcga ggtcggctcg atcgtgttgt
     3421 ggccgcaggt cgtcaaggag gtcgcgccga tcccggtgct ggccgcgggc ggcatcggca
     3481 gcggccagca gatcgccgcc gcactggcgc tcggcgcgca gggcgcgtgg acgggttcgc
     3541 agtgggtgat ggtcgaggag tcagagcaca cgcccgtgca gcacgccgcg tacgcgaag
//
LOCUS       DASYLX010000607         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2115709,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000607 DASYLX010000000
VERSION     DASYLX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115709"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1536)
                     /locus_tag="VGP27_14465"
     CDS             complement(154..1536)
                     /locus_tag="VGP27_14465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7852535.1"
                     /translation="MGDGRTRVLIIGGGFGGLFCARRLGRVDVDVTLLDRAACHVFQP
                     LLYQCATGTLSIGQISRSLREELARHHNVTTLLGEAVDLDADAHRITARRPDETTFTI
                     DYDVLIVAVGMRQSYLGHQEFAQWAPGMKTLDDALSVRRRLFGAFEMAETLPPGPERD
                     AWLTFAIAGGGPTGVELAGQIREMATRALAKEFHSIEPEDARVLLFDGGSCVLGSFAP
                     ALTDKATTILQRLGVELHMGVHVTDVRHDGVTVTPKTGGPPEDFAARTVLWTAGVEAV
                     PFARHLAKVLDVKSDHAGRIEVEADLSVPGYPEIFVIGDLVGRDKLPGVAENAMQGGL
                     HAAACIRRDLAGKPRRPYRYRDLGSAAYISRGHALLQAGPVKLAGFVGWLGWGLIHIA
                     FLTGLENRISTVATWLASIARARRTDRTFTLGSTTSPEHPYTWLKFMHPNMLDASPDS
                     GEATPTHRIS"
     gene            complement(1681..>1998)
                     /locus_tag="VGP27_14470"
     CDS             complement(1681..>1998)
                     /locus_tag="VGP27_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852536.1"
                     /translation="PDCVSIDNRPGRAYEGDMIPYVNAMWEVAPDIMMCIEVVHGLTS
                     RGALVTWASYGTSQEGFSFESRGVSILMLEGDLIAHGEIFDEAHLDAALAKFEELNRA
                     VHS"
BASE COUNT          350 a          638 c          659 g          351 t
ORIGIN      
        1 cgaccgcaga gctgatgcgc gactgcgggt tctccatcgc agccgacgac gacgtgtggc
       61 gcggcatgcc ttccatcgtc cgcccgctgg tcgtcggacg ggcgactcgg tgatctgtcc
      121 gtcgtcgact tcaccgagcc caacaagaag cgctcacgaa atgcgatgag tgggcgtcgc
      181 ctcgcccgaa tctggcgacg cgtcaagcat gttggggtgc atgaatttca accaggtata
      241 cgggtgctcg ggcgaagtcg tgctacctag ggtgaacgtt cggtcggtgc ggcgcgcgcg
      301 tgcgatggag gccagccagg tcgcgactgt gctgatgcgg ttctccaggc cagtgagaaa
      361 cgcgatgtga atgagtcccc atcccagcca tccgacgaac ccggcgagtt tgacggggcc
      421 ggcctgcagc agggcatgtc cacggctgat gtaggccgca gagccgagat cgcggtagcg
      481 atagggccgg cgcggtttgc cggcaaggtc acgacgaatg caagcggcag cgtggagtcc
      541 gccctgcatc gcgttctcgg cgactcccgg cagtttgtcg cgaccgacga ggtcaccgat
      601 gacgaagatc tccgggtacc cgggcacgga caaatcggct tccacttcga tgcgtccggc
      661 gtggtcggat ttgacgtcga gcaccttcgc gaggtgccgc gcgaagggca cggcctcaac
      721 gcccgccgtc cagagcacgg tccttgccgc gaagtcttca ggtgggccgc ccgtcttcgg
      781 tgtgacggtc acgccgtcgt gccgcacgtc ggtgacgtgc acacccatgt gcagctcaac
      841 gccgagccgc tgcagaatcg tggtggcttt gtcggtgagg gcgggcgcga agctgccgag
      901 aacgcagctg cccccgtcga aaagcagtac acgcgcgtcc tcgggttcga tgctgtggaa
      961 ctccttggcc agcgcccggg tcgccatttc gcggatctgg cccgccagct ccacaccggt
     1021 ggggccgcca ccggctatcg cgaaggtgag ccacgcgtcg cgctccggcc ccggaggcag
     1081 ggtttcggcc atctcgaacg cgccgaacag tctgcgccgg acgctcaacg cgtcgtcgag
     1141 ggtcttcatc cccggcgccc actgcgcgaa ctcctgatga cccaaataag attggcgcat
     1201 tccgacggcg actatcagta cgtcgtagtc gatcgtgaag gtggtctcgt cggggcggcg
     1261 ggcggtgatc cggtgcgcgt cggcgtcgag gtccactgct tcgcccagca gggtggtgac
     1321 gttgtggtga cgcgcgagtt cctcgcgcag cgagcggctg atctgcccga tgctcagggt
     1381 tccggtggcg cactggtaga gcagcggctg aaacacgtgg caggcggcgc gatcgagcaa
     1441 cgtgacgtcg acatcgacac gtccgagcct gcgcgcgcag aacaacccgc cgaatccgcc
     1501 cccgatgatc agcaccctcg tccggccgtc acccatcacg tctccttttc gcagtcgcca
     1561 acaccaatct catcacggtg tacctgtcca gtgtccgtgt gaggcactgt gcgtgacgag
     1621 ggaaggtccg agaaagcgtt ggaacgaggc gttcaccgcc acgaacaagc gggcgcccgt
     1681 ttaggaatga acggcccggt tgagttcctc gaacttcgca agcgcggcat cgaggtgtgc
     1741 ctcgtcgaaa atctcgccgt gggcgatcaa gtcgccctcg agcatcagaa tgctgacgcc
     1801 ccgcgattcg aaactgaagc cctcttgcga ggttccgtac gacgcccagg tgacaagggc
     1861 tccgcgactg gtcagcccat gcaccacctc gatgcacatc atgatgtcag gagctacctc
     1921 ccacatggcg ttgacgtagg gaatcatgtc gccttcgtaa gctctgccgg gacgattgtc
     1981 gatgctgacg cagtccgg
//
LOCUS       DASYLX010000608         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3322471,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000608 DASYLX010000000
VERSION     DASYLX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322471"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGP27_14475"
     CDS             complement(<1..237)
                     /locus_tag="VGP27_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEV7852537.1"
                     /translation="MTDDREDLPWELFGSAADELAAGIAADGFIPDLILAVARGGLFV
                     AGALGYALGVKNVAVINVEFYTGVDERLPVPVVLP"
     gene            complement(234..>1600)
                     /locus_tag="VGP27_14480"
     CDS             complement(234..>1600)
                     /locus_tag="VGP27_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7852538.1"
                     /translation="GLSVRVIEGQPTAGGGARSAPDPDFPDVRHDVCSAVHPLGVASP
                     FFAQFDLAARGVALVAPEVSYANPLPGRPAAVAYRSLERTCEELDDGASWRRLFGPLA
                     ERADGVVGFFLGDKRSLPPELVTTVRTGLRVLAQGSPAWGLLRGQDAQALFTGVGAHA
                     ISKMPSPVNSGAGVMLGTLAHTVGWPVPVGGTQTIPDALIADLLSHGGELVLGEPVTS
                     PPDGVTIYDTAPTALLDIYGDAVPLRYAKALRRYRFGPGVCKVDFVLSGEIPWRDARV
                     GQAPTVHLGGTRAQMAAGEREIAAGRHAELPMTLVALPHLADPSRIDGQGRRPLWTYA
                     HVPAGSTADLTETITAMFEAAAPGFRDLVVTSHCVPAAQMSDHNANYVGGDIIVGGAT
                     LFAAMVGPTLRLNPWTTPIPKAYLCSSATPPGTGVHGMAGYYAARTVLRREFGIKALP
                     ELSP"
BASE COUNT          261 a          560 c          554 g          225 t
ORIGIN      
        1 aggcagcacc accggcaccg gtaggcgttc atcgaccccg gtgtagaact cgacgtttat
       61 gaccgccacg ttcttcaccc ccaacgcgta gccgagcgca ccggcgacga acagtccgcc
      121 gcgtgccacg gccaggatca ggtcgggtat gaacccgtcg gcggcgatgc cggcggccaa
      181 ctcgtctgcg gccgagccga acagctccca cggcagatct tcccggtcgt ctgtcatggt
      241 gacagctcag gcagcgcttt gatcccgaac tccctgcgca gcactgtgcg ggcggcgtag
      301 tagccggcca tcccgtgcac acccgtgccg ggcggtgtcg ccgatgagca cagatacgcc
      361 tttgggatgg gggtcgtcca cggattgagc cgcagcgtcg ggccgaccat tgcggcgaac
      421 agggtggctc cgcccacgat gatgtcccct ccgacgtaat tcgcgttgtg gtccgacatc
      481 tgggccgcgg gcacgcagtg cgacgtgacg accaggtccc ggaaaccggg cgcggcggcc
      541 tcgaacatcg cggtgatcgt ctcggtcagg tccgcggtcg atccggccgg aacatgtgcg
      601 taggtccaca acggcctgcg gccctgaccg tcgatgcgcg acgggtcggc caggtgcggc
      661 agcgcgacga gggtcatcgg cagttcggcg tgccgcccgg cggcgatctc acgttcaccc
      721 gcggccatct gcgccctcgt cccgcccaga tgtacggtcg gtgcctgacc gacccgcgcg
      781 tcgcgccacg gaatctcgcc ggagagcacg aagtcgacct tgcatacacc gggcccgaaa
      841 cggtagcgcc gcaacgcctt cgcgtaacgc aacggaaccg cgtcgccgta gatgtcgagc
      901 agagccgtcg gcgcggtgtc gtagatcgtc acgccgtcgg gcggggaggt gacgggttca
      961 cccagaacca attcgccacc gtgggagagc agatcggcga tcaacgcgtc ggggatcgtc
     1021 tgtgttccgc cgaccggcac cggccagccc accgtgtgcg ccaacgtgcc cagcatgact
     1081 ccggcgccgc tgttgaccgg tgacggcatc ttcgaaatag cgtgcgcgcc aacgccggtg
     1141 aacagcgctt gggcgtcttg tccgcggagc agaccccagg cggggctgcc ttgcgccagc
     1201 acccgcaggc cggtccgcac ggtggtcacc agttccggtg gcagggacct cttgtcgccg
     1261 aggaagaatc ccacgacgcc gtcggcgcgt tcggccagtg gcccgaagag acgccgccac
     1321 gacgcgccgt cgtcgagctc ctcgcaggtg cgctccagcg agcggtacgc caccgcggcg
     1381 ggccggccgg gcaatggatt ggcataggag acttccggcg cgacgagcgc caccccgcgc
     1441 gccgccaggt cgaactgcgc gaagaacggc gaggccacgc cgagcggatg caccgcggag
     1501 cagacgtcgt ggcgcacgtc ggggaagtcg gggtcgggcg cgctgcgggc gccgccaccc
     1561 gcagtcggct gaccttcgat gacgcgcacc gaaagacccg
//
LOCUS       DASYLX010000609         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3761788,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000609 DASYLX010000000
VERSION     DASYLX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761788"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGP27_14485"
     CDS             complement(<1..620)
                     /locus_tag="VGP27_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7852539.1"
                     /translation="MVIKDPHADSGMEDFGYKQSLDRSIGKFASFAAGVSYISILTGT
                     FQLFYFGFGTAGPAYLWSWPLVFVGQMAVALCFMELAAKYPVAGSVYNWSKKLGSRLV
                     GWSSGWLMLTASIVTISAVVLAYQLNLPRIWSGFQIIGDGTGEYDFAANAVVLGSVLI
                     VFTTVINALGVRLMARINSAGVFIELIAAVLIAIILALNVKRGPDI"
     gene            complement(633..2123)
                     /locus_tag="VGP27_14490"
     CDS             complement(633..2123)
                     /locus_tag="VGP27_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7852540.1"
                     /translation="MNGEPDLLIGGKWRHASDGGTREIINPADGSVAAVVDEATPSDA
                     RAAVAAARNAFDHGGWRATPVADRAALLDRVADLLQRDKEDLASLETRDTGKTLAESR
                     IDIDDVTSVFRYYGKLAAVESDRIVDVGDPAIISRVVREPIGVCVLIAPWNYPLLQMS
                     WKIAPALAAGCTMVAKPSEVTPLTTIAFVHLLQEAGVPDSVVNLVQGSGATLGPALTD
                     TPDVDFISFTGGVATGKTISKVAAEHVTKVAVELGGKNPHIVFADIADSGDWDAAVDH
                     VLTGVFLHSGQVCSAGTRLIVEESVADDFVAELARRAEKIRMGDGMDPASETGPLVSE
                     QQRAKIENHVQQGISEGAKLVTGGARPSDPALAKGSFYSPTIFDRCDRSMRIVQEETF
                     GPILTVERFTTEAEAIELGNDTEYGLAAGVRTSDVARGERVVRALRHGTVWLNDFGYY
                     TAAAEWGGFGKSGNGRELGPTGLAEYQELKHIWHNTAPMPAGWFKG"
     gene            complement(2166..>2988)
                     /locus_tag="VGP27_14495"
     CDS             complement(2166..>2988)
                     /locus_tag="VGP27_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7852541.1"
                     /translation="WTYALDGKLMAAPNIASLTDETGAAIDRYRYGLVPVALTEWLGY
                     AWVCISDTPPPFEDVVAETTRILGDSDVINRYGIGGLDVGHRVVYDVAANWKLIVENF
                     MECYHCSSIHPELVGVLPEFAKGMAAQANIGLGAQFGSEITGFTVDGTAGFDRLPGIT
                     DEQDRRYYAITVKPTVFINLVPDHIIFHRMFPVSASRTIVECDWLYTADVVSSGRDVS
                     HSVELFHRVNEQDFGACERTQPSMSSRAYRNGGVLVPAEHHIAEFHEWVVSRLGA"
BASE COUNT          520 a          969 c          977 g          522 t
ORIGIN      
        1 aagatgtcgg gcccgcgctt gacgttcaag gccaggatga tggcgatcag cacggcagcg
       61 atcagctcga tgaacacgcc cgcactgttg atcctggcca tcagcctcac cccgagagca
      121 ttgatcaccg tggtgaatac gatcaggacg ctgccgagaa cgactgcgtt ggcggcgaag
      181 tcgtactcgc cggtgccgtc gccgatgatc tggaatccgc tccatatcct cggcaggttg
      241 agttggtaag ccagcaccac cgcggagatc gtcacgatcg acgcggtcag catcagccaa
      301 cccgacgacc atccgaccag gcggctaccc agcttcttgc tccagttgta gaccgatccc
      361 gcaaccggat acttggcggc cagctccatg aagcacagcg cgacggccat ctggccgacg
      421 aacaccaacg gccacgacca cagatatgcg ggtcccgcgg tgccgaagcc gaagtagaac
      481 aactggaacg tgccggtgag gatggagatg tagctgacac ccgcggcgaa gctggcgaac
      541 tttccgatgc tgcggtccag cgactgctta tagccgaagt cctccatgcc gctgtcggcg
      601 tgcggatcct tgatcaccat gactaattct ccttaaccct tgaaccagcc ggccggcatt
      661 ggcgcggtgt tgtgccagat gtgcttgagc tcttggtatt ccgccagccc ggttggtccg
      721 agctcgcggc cgttgcccga cttcccgaat ccgccccact ccgccgccgc ggtgtagtaa
      781 ccgaaatcgt tgagccacac cgtgccgtgc cgcagtgccc gcaccacacg ctcgccacgc
      841 gcgacgtccg acgtccgaac gcccgccgcg agcccgtatt ccgtgtcgtt gccgagttct
      901 atggcctcgg cttcggtggt gaaccgctcg accgtgagaa tcggcccgaa ggtctcctcc
      961 tggacgattc gcatcgaccg atcgcaccgg tcgaagatcg tgggcgaata gaaactgccc
     1021 ttggcaagcg ccgggtcgct gggccgggcg ccgccggtaa ccaacttggc gccctcggaa
     1081 atgccttgct ggacatgatt ttcgatcttg gcacgctgtt gttcggagac caacgggccg
     1141 gtctcgctgg ccgggtccat accgtcgccc atccggatct tctcggcgcg cctggccagc
     1201 tcggcgacga agtcgtcggc gacggactcc tcgacgatca gccgggtgcc cgccgagcag
     1261 acctgtccgg agtgcaggaa cacaccggtg aggacgtggt cgacggctgc gtcccaatcg
     1321 cccgagtcgg cgatgtcggc gaacacgatg tgcggattct tgccaccgag ttcgacggcc
     1381 accttggtga cgtgctcggc ggcgaccttg gagatggtct tgccggtggc cactccgccg
     1441 gtgaacgaga tgaagtcgac gtcgggggta tcggtcagtg cgggccccag cgtggcgccg
     1501 ctgccctgca ccaggttcac caccgaatcc ggcacaccgg cctcttggag taggtgcacg
     1561 aacgcgatcg tcgtcagcgg ggtgacctcg ctcggcttgg ccaccatcgt gcaaccggcg
     1621 gccagcgcgg gcgcgatctt ccatgacatc tgcagcagcg gatagttcca cggcgcgatc
     1681 agcacgcaca ccccgatggg ctcgcgcacc acccggctga taatggcggg atcgccgacg
     1741 tcgacgatgc ggtccgactc gacggcggca agctttccgt agtagcggaa gaccgacgtg
     1801 acgtcgtcga tgtcgatgcg gctctcggcc agcgtcttgc cggtgtcccg tgtttcgagc
     1861 gacgccagat cttctttgtc ccgctggagc aggtcggcga cccggtcgag cagggcggca
     1921 cggtcggcga ccggggttgc ccgccacccg ccgtggtcga aggcgtttcg cgcggctgcg
     1981 acggcggcgc gcgcgtcgga gggggtggcc tcgtcgacca ccgcggcgac gctgccgtcg
     2041 gcagggttga tgatctcgcg agtcccgcca tcggaggcgt gacgccactt cccgccgatc
     2101 agtagatcgg gttcaccgtt catgttccgc tcacctcgct ttgcggagcc gctccggcgg
     2161 cgacatcagg caccgagacg ggacactacc cactcatgga actccgcgat gtgatgttcg
     2221 gcaggtacga ggacaccacc gttccggtag gcccgggagg acatcgatgg ctgagttcgc
     2281 tcgcacgcac cgaaatcctg ctcgttgacc cggtggaaca gctccaccga gtgggagacg
     2341 tcgcggcccg aggacaccac gtcggcggtg tagagccagt cgcattcgac gatggtccgc
     2401 gacgccgaca ccgggaacat ccgatggaag atgatgtggt cgggaaccag gttgatgaac
     2461 accgtcggtt tgacggtgat cgcgtaatag cgacggtcct gttcgtcggt gatgccgggc
     2521 aggcggtcga agcccgccgt gccgtcgacg gtgaaaccgg ttatctcgga accgaattgg
     2581 gcacccaggc cgatgttggc ctgtgcggcc atccctttcg cgaactcggg tagcacaccg
     2641 acgagttcgg gatggatcga ggagcagtgg tagcactcca tgaagttctc gacgatcagc
     2701 ttccagttgg ccgccacgtc gtagaccacg cgatggccga cgtcaaggcc gccgatgccg
     2761 tagcggttga tgacgtcgga gtcgccgagg atcctggtcg tctcggccac gacgtcctcg
     2821 aagggcggcg gcgtgtcgga gatgcagacc caggcgtagc cgagccactc ggtcagcgcg
     2881 acgggaacaa ggccgtatcg gtagcggtcg atcgccgccc cggtctcgtc ggtgagcgac
     2941 gcgatgttgg gcgcggccat cagcttgccg tccagggcat acgtccag
//
LOCUS       DASYLX010000610         4882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2116014,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000610 DASYLX010000000
VERSION     DASYLX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116014"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGP27_14500"
     CDS             <1..360
                     /locus_tag="VGP27_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV7852542.1"
                     /translation="PSERAQIIAGLPSGSLDNAIGQIEELARSSVLPTCPTRTPPPPT
                     PTVAPPPPPPAPAETPAPETPTDPNIPAAGPTRGPLPGPAPETPTTVTAPPSPTPTAT
                     ALPPPPSEPGKTCRTVS"
     gene            357..1772
                     /locus_tag="VGP27_14505"
     CDS             357..1772
                     /locus_tag="VGP27_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV7852543.1"
                     /translation="MTTQPQPPVTVLPPLPNRRNAELLLLGFATVITTVALLIVEANQ
                     EQGLSWDLASYTLAYIALFAGAHLTIRRFAPYADPLLLPVVALLNGLGLVMIHRLDLA
                     AGDVTSGGVRGPSANQQMLWTLVGVIAFSLVVVFLRDHRVLARYGYVCGVAGLILLAI
                     PAVLPASMSEQNGAKIWIALPGFSIQPAEFSKILLLIFFAAVLVSKRSVFTSAGKHVL
                     GMDLPRPRDLAPLLAAWILSVGVMIFEKDLGTSLLLYASFLVLVYVATERFSWVVIGL
                     ALFAAGSVAAYYIFAHVQTRVQNWLDPFADPDGAGYQMVQSLFSFATGGIFGTGLGNG
                     QPGVVPAASTDFIIAAVGEELGLVGLAGVLMLYTIVIVRGLRTAIAVRDSFGKLLAAG
                     LASTLAIQLFIVVGGVTKLIPLTGLTTPWMSYGGSSLLANYVLLAILIRISHAARRPI
                     VTRPPNKSPIASAGTEVIERV"
     gene            1769..3244
                     /gene="pbpA"
                     /locus_tag="VGP27_14510"
     CDS             1769..3244
                     /gene="pbpA"
                     /locus_tag="VGP27_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-transpeptidase PbpA"
                     /protein_id="HEV7852544.1"
                     /translation="MNTSLRRISVMIMVLIVLLLANATLTQVFTADGLRADPRNQRVL
                     LDEYSRQRGQISAGGQLLAYSVSTDGRFRFLRVYPNPFTYAPVTGFYSLRFSSNGLER
                     AEDTILNGSDERLFGRRLADFFTGRDPRGGNVATTIKPQVQQAAWDAMQDGCSGPCTG
                     AVVALEPSTGKILAMVSAPSYDPNLLASHDVNAQSDAWQQLRDNPGNPLLNRAISETY
                     PPGSTFKVITTAAALQSGATPNTQLPSAARIPLPDSTATLENFGGATCGGGPTASLRE
                     AFAKSCNTAFVQLGIDTGADALKNTAQAFGLDDSPAPIPLQVAESTVGPISDAAALGM
                     SSIGQKDVALTPLQNALVAATIANKGITMQPYLVDSLKGPDLSNIETTSPHEQRRAVS
                     GQVADTLTDLMVDAEQVTQQKGAIAGVQIASKTGTAEHGTDPRNTPPHAWYIAFAPAR
                     APKVAVAVLVENGGNRLSATGGALAAPIGRATIAAALREGS"
     gene            3241..4548
                     /locus_tag="VGP27_14515"
     CDS             3241..4548
                     /locus_tag="VGP27_14515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7852545.1"
                     /translation="MSPRVGVTLSGRYRLQRLIATGGMGQVWEAVDSRLGRRVAVKVL
                     KAEFSSDPEFVERFRAEARTVAMLNHPGIASVYDYGETDMDGEGRTAYLVMELINGEP
                     LNSVLKRTGRLSLRHSLDMLEQTGRALQVAHTAGLVHRDVKPGNILITPTGQVKLTDF
                     GIAKAVDAAPVTQTGMVMGTAQYIAPEQALGHDATAASDVYALGVVGYEAVSGKRPFT
                     GDGALTVAMKHIKETPAPLPADLPPNVRELIEITLVKNPGMRYRSGGPFADAVAAVRA
                     GRRPPRPNAAPTIGRAAPAAVGSAPPSAAMTARGPAAAARARPATGGHRPPPPRRTFS
                     SGQRALLWAAGVLGALAIAIAILIVLNAQDKKDQQSPPPTVTDTITQTTPQGAPPLVT
                     VPGWTDDGSIGHRGEQIGAYRPNAIAPSALLRAASSDLEQTLE"
     gene            4545..>4882
                     /locus_tag="VGP27_14520"
     CDS             4545..>4882
                     /locus_tag="VGP27_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7852546.1"
                     /translation="MTTPQHLSDRYELGDILGFGGMSEVHLARDVRLHRDVAVKVLRA
                     DLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPNGPLPYIVMEYVDGITLRD
                     IVHTDGPMES"
BASE COUNT          757 a         1782 c         1571 g          772 t
ORIGIN      
        1 ccgtcggaac gcgctcagat catcgccggc ctgccgtcgg gcagcctcga caacgccatc
       61 ggtcagatcg aggagctcgc ccgcagctcg gtcctgccga cctgccccac gcgcaccccg
      121 cccccgccga ctccgaccgt cgcgccgccg cctccgccgc ccgccccagc ggagacgccc
      181 gcacccgaaa cgcccaccga tcccaacatc ccagcggccg gcccgacgcg cggaccgctg
      241 cccggtccgg ctcccgagac gccgaccacc gtcaccgctc ccccgtcacc gacaccgacg
      301 gccaccgcct tgccgccgcc gccctccgag ccgggcaaga cctgccggac cgtgtcatga
      361 cgacacaacc ccagccgccc gtcaccgtat tgccgccgct gcccaaccgg cgcaatgccg
      421 agctgttgct gctggggttc gcgaccgtca tcaccaccgt ggccctcctc atcgtcgagg
      481 ccaaccagga gcagggcctg agctgggacc tggcctccta cacgctggcc tacatagcgt
      541 tgttcgccgg cgcgcacctg acgatccggc gtttcgcgcc ctacgccgac ccgcttctgc
      601 tgccggtggt cgccctgctg aatgggttgg ggctggtgat gattcaccgc ttggacctcg
      661 ccgccgggga cgtcacctcc ggcggggtgc gcgggccgag cgccaaccag cagatgctgt
      721 ggacgctggt cggcgtcatc gcgttctcct tggtggtggt cttcctgcgt gaccatcgcg
      781 tcctggcgcg ctacggctac gtctgcggcg tcgcaggcct catactgctg gcgattcccg
      841 ccgtactgcc tgcgtcgatg tcggagcaga acggcgccaa gatctggatt gcgctgcccg
      901 gcttctcgat tcagcccgcc gagttctcca agatcctgct gctgatcttt ttcgccgccg
      961 tcctggtgtc caagcgcagc gtgttcacca gtgccggtaa gcatgtgctc ggcatggacc
     1021 tgccccggcc tcgcgacctg gcgccactgc tcgcggcgtg gatcctctcg gtcggtgtca
     1081 tgatcttcga gaaggacctc ggcacctcgc ttctgctcta cgcgtcgttc ctggtgctgg
     1141 tgtatgtcgc caccgagcgg ttcagctggg tcgtcatcgg tctcgccctg ttcgcggcgg
     1201 gaagcgttgc ggcgtattac attttcgccc acgtgcagac ccgggtgcag aactggttgg
     1261 accccttcgc cgatcccgac ggcgccggct accagatggt gcagtcactg ttcagcttcg
     1321 ccaccggcgg gatcttcggc accggccttg gcaacggtca gcccggcgtt gtgcccgcgg
     1381 cgtccacgga cttcatcatc gctgcggttg gtgaggaact cgggctcgtc gggctcgccg
     1441 gtgtgctgat gctgtacacg atcgtcatcg tccgcggcct tcgcactgcg atcgcggtgc
     1501 gcgacagctt cggcaagctg ctcgccgccg gtttggcgtc caccctggcg atccagctct
     1561 tcatcgtcgt cggcggcgtg accaagctga tcccgttgac gggtctcacc acgccgtgga
     1621 tgtcctacgg cggttcgtcg ctgctggcca actatgtgct gctggcgatc ctgatccgca
     1681 tctcgcatgc cgcgcgccgc ccgatcgtca cccggccgcc gaacaagtcg ccgatcgcgt
     1741 cggccggcac cgaggtgatc gagcgcgtat gaacacctcg ctgcgccgga tctcggtcat
     1801 gatcatggtg ttgatcgtgc tgttgctggc caacgccacg ctcacacagg tcttcacggc
     1861 cgacggcctt cgcgccgatc cacgcaacca gcgcgtgctg ctcgacgagt actcgcgcca
     1921 gcgcggccag atctcggcgg gcggtcagct gctggcgtac tcggtgtcga cggacggccg
     1981 gttccggttc ctgcgggtct atccgaaccc cttcacctac gcgcccgtca ccggcttcta
     2041 ctcgctgcgg ttctcgagca acggcctgga acgcgcggag gacaccatcc tcaacggctc
     2101 cgacgaacgg ctgttcggtc ggcggctcgc cgacttcttc accggtcgcg acccgcgcgg
     2161 cggcaacgtg gcgaccacca tcaaacctca agtgcagcag gcggcgtggg atgcgatgca
     2221 ggacggctgc agcggcccgt gcaccggcgc ggtggtggca ctggaaccgt cgaccgggaa
     2281 gatcctggcg atggtgtcgg ctccttcgta cgaccccaac ctgctggcca gccacgacgt
     2341 caacgcgcag tcagatgcct ggcagcagct gagagacaac cccggtaacc ctctgctgaa
     2401 tcgggccatc tcagagacgt acccgccggg gtccacattc aaggtcatca ccacggccgc
     2461 tgcactgcag agcggcgcga caccgaacac tcagttgccg tccgctgctc ggatcccgtt
     2521 gccggacagc accgcaacgc tggagaactt cggcggcgcg acgtgcggtg gcggcccgac
     2581 cgcctcgctc cgcgaggcgt tcgccaaatc gtgcaacacc gcattcgttc aactcggcat
     2641 cgacaccggc gccgacgcgc tgaagaacac cgcgcaggct ttcggcctgg acgacagtcc
     2701 cgcgccgatt ccgctccagg tcgccgagtc caccgtgggc ccgatcagcg atgccgccgc
     2761 cctgggaatg tcgagcatcg ggcagaagga cgtcgcgctc acaccgctgc agaacgcgtt
     2821 ggtggccgcc accatcgcga acaaggggat aacgatgcag ccgtacctgg tcgacagcct
     2881 caaggggccc gacctgtcga acatcgagac gacgtcgccg cacgaacaac gccgtgcagt
     2941 gtcggggcag gtcgccgata cacttactga cttgatggtc gacgccgagc aggtgacgca
     3001 gcagaaggga gccatcgccg gcgtgcagat cgcatcgaag acaggcaccg cggagcacgg
     3061 gaccgacccg cgcaacaccc cgccgcacgc gtggtacatc gcgtttgcgc ctgcccgcgc
     3121 accgaaagta gcggtagccg tgctggtgga gaacggcggc aaccggcttt cggccaccgg
     3181 cggcgcgctg gccgcgccca tcggcagggc cacgatcgca gcagcactgc gggagggctc
     3241 atgagccccc gcgtcggagt caccctgtcg ggccgttacc gcctgcagcg gctgatcgcc
     3301 accggcggca tggggcaagt ctgggaggcc gtcgattcgc ggcttggccg ccgggtcgcg
     3361 gtgaaggtgc tcaaggccga gttctcgtcc gatcccgagt tcgtcgagcg gttccgcgcc
     3421 gaggcgcgca ccgtcgcgat gctcaaccat ccgggcatcg ccagcgtcta cgactacggc
     3481 gaaaccgaca tggacggcga gggccgcacc gcgtatctgg tgatggagct gatcaacggc
     3541 gaaccgctga actcggtgct caagcgcacc ggcaggctct cgctgcggca ctcgctggac
     3601 atgctcgagc agaccggtcg ggcgctgcag gttgctcaca ccgccgggtt ggtgcaccgc
     3661 gacgtcaagc cgggcaacat cctgatcacg ccgaccggcc aggtgaagct caccgacttc
     3721 ggcatcgcga aggccgtcga cgccgcaccc gtcacccaga ccggcatggt gatgggcacc
     3781 gcccagtaca tcgcgcccga gcaggcgctg ggtcacgacg cgaccgcggc cagcgatgtc
     3841 tacgcgctgg gagtcgttgg ctacgaagct gtttcgggca agcgtccgtt caccggcgac
     3901 ggtgccctta cggtcgcgat gaagcacatc aaggagacgc ctgcgccgct gcccgccgat
     3961 ctgccgccca acgtgcggga gctgatcgag atcaccctgg tgaagaaccc gggcatgcga
     4021 taccgctccg gcggcccgtt cgccgacgcg gtcgccgccg tgcgggcagg gcgacgtccg
     4081 ccgcggccga atgcggcgcc gacgatcggc cgtgccgcac cggctgccgt gggatccgct
     4141 ccgcccagcg ccgccatgac cgcgcggggg ccggccgccg cggcgcgcgc ccggcccgcg
     4201 accggcgggc accgtcctcc cccaccgcga cgaacgttct cgtccgggca gcgggcgctg
     4261 ctgtgggccg cgggtgtgct cggagcgctg gccatcgcca tcgcgatcct gatcgtgctg
     4321 aacgcccagg acaagaaaga ccagcaatcg ccgccgccga cggtcaccga taccattacc
     4381 cagaccactc cgcagggggc tccgccgctg gtcacggtgc cgggctggac cgatgacggg
     4441 tccatcggtc atcgtggaga gcaaatcggg gcataccggc ccaacgcgat cgcgccgtcc
     4501 gcactgctgc gggcggcgtc ctcggatcta gaacagacat tggaatgacc accccgcagc
     4561 acctgtccga ccgctacgaa ctcggcgaca tcctcggctt cggcggcatg tccgaagtgc
     4621 acctcgcgcg cgatgtgcgc ctgcatcgag acgtggccgt caaagtgctg cgcgccgacc
     4681 tggcccgcga cccgagcttt tatctccggt ttcgccgcga agcgcagaac gccgccgcgc
     4741 tgaaccaccc ggcgatcgtc gcggtgtacg acaccggtga ggccgagacg ccgaacggcc
     4801 cgctgcccta catcgtgatg gagtacgtcg acggcatcac gctgcgcgac atcgtgcaca
     4861 ccgacggccc gatggagtcg ca
//
LOCUS       DASYLX010000611         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_359230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000611 DASYLX010000000
VERSION     DASYLX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1546
                     /locus_tag="VGP27_14525"
     CDS             <1..>1546
                     /locus_tag="VGP27_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEV7852547.1"
                     /translation="FTLPACTVDGGMPVVTVDDASNAMRSVLAIAAGADGPDALPTVV
                     NNNTSVTVDWDPEKVADHTGVTATFGAPLAPGLTVVPDALVGLCWPAVFSVIGSARTD
                     EGFPVVEGLLSLVHLDHAAHLLAELPKTPAQLTITATAFAATDTEVGRVVPVQATIMD
                     AEGTELARLEERFAIRGRTGAVELTDPPRAGGAMTDNATDTPRRRRRDVTVTAPGDMS
                     AFAVVSGDHNPIHTDRAAALLAGLESPIVHGMWLSAAAQQVVTATDGRAIPPARLIGW
                     TARFLGMVLPDDEVDIRVDRVGIDRGAEIVEIAARVGSNLVMSATAQLAAPLTVYAFP
                     GQGIQHKGMGMEVRARSKAARKVWDSADKFTRETLGFSVLHVVRDNPTSLIASGVHYQ
                     HPEGVLYLTQFTQVAMATVAAAQVAEMREQGAFVEGAVACGHSVGEYTALACVSGVYE
                     LEALLEVVFHRGSKMHDIVPRDEFGRSNYRLAAIRPSQIDLDDSDVVDFVAEISERTG
                     EFLQIV"
BASE COUNT          230 a          548 c          528 g          240 t
ORIGIN      
        1 ggttcacatt gccggcctgc acggtggacg gcggcatgcc ggtggtcacc gtcgacgacg
       61 cgtcgaacgc gatgcgctcc gtgctggcga tcgccgccgg cgccgacggc cccgacgcgc
      121 tgccgacggt ggtgaacaac aacacctcgg tcactgtcga ctgggacccg gagaaggtcg
      181 ccgaccacac cggcgtgacc gcgacgttcg gtgcgcctct ggcgcccggc ctcacggtgg
      241 tccccgatgc gctcgtgggc ctctgctggc ctgcggtgtt ctccgtcatc gggtcagcgc
      301 ggaccgacga gggcttcccg gtcgtcgagg gtctgctcag cctggtccac ttggatcacg
      361 ccgcgcacct gttggccgag ctgccgaaaa ccccggctca attgaccatc accgcaacgg
      421 ctttcgccgc caccgacacc gaggtcggtc gcgtggtgcc ggtgcaggcc accatcatgg
      481 acgccgaggg taccgagctg gccaggctcg aggagcggtt cgcgatccgc ggccgcacgg
      541 gcgccgtcga actcaccgac ccgccgcggg ccggcggcgc gatgaccgac aacgcgaccg
      601 acacgccgcg gcgccgtcgc cgcgacgtca ccgtcaccgc accgggcgac atgagcgcgt
      661 tcgcggtcgt ctccggtgac cacaatccga tccacaccga ccgtgccgcg gcgctgctgg
      721 ccgggctgga atcgcccatc gtgcacggca tgtggctgtc cgccgccgcc cagcaggtcg
      781 tcacggccac cgacggcagg gccatccccc ctgctcgtct gatcggttgg accgcccgct
      841 tcctcggcat ggtgctgccc gacgacgagg tcgacatccg cgtcgatcgc gtcggaatcg
      901 accgtggtgc agagattgtc gagatcgccg cgcgcgtcgg gtccaatctg gtgatgtcgg
      961 cgacggcaca gctggccgct ccgttgacgg tgtacgcgtt cccgggccag ggcatccagc
     1021 acaagggcat gggcatggag gtccgcgccc ggtccaaggc ggcccgcaag gtgtgggact
     1081 ccgccgacaa gttcacccgc gaaacgcttg gcttctcggt gctgcacgtg gtgcgcgaca
     1141 acccgacgag cctgatcgcc agcggtgtgc actatcagca ccccgagggc gtgctgtatc
     1201 tgacgcagtt cacccaggtc gcgatggcga cggtggcggc agcgcaggtc gccgagatgc
     1261 gtgaacaggg ggccttcgtc gagggcgccg tcgcctgcgg ccactcggtc ggcgagtaca
     1321 ccgcgctggc ctgcgtcagc ggtgtgtacg aactcgaggc gctgctcgag gtggtgttcc
     1381 accgcggctc gaagatgcat gacatcgtcc cgcgcgacga gttcggccgg tccaactacc
     1441 ggctggccgc gatccggccg tcgcagatcg acctcgacga ctccgacgtc gtcgacttcg
     1501 tcgccgaaat ctccgagcgc acaggtgaat tcctgcagat cgtgaa
//
LOCUS       DASYLX010000612         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3651143,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000612 DASYLX010000000
VERSION     DASYLX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651143"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGP27_14530"
     CDS             <1..275
                     /locus_tag="VGP27_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7852548.1"
                     /translation="PVLFGHIGEGNLHLNVVRCALDGEREARLYSAMMALIARCGGNV
                     SSEHGVGSRKRDYVPMSRTVADIAAMRLVKTAFDPGGYLNAAVLFD"
     gene            complement(327..2465)
                     /locus_tag="VGP27_14535"
     CDS             complement(327..2465)
                     /locus_tag="VGP27_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852549.1"
                     /translation="MTTALRGRPTGGAAPVADPWHALWAMMVGFFMILVDATIVAVAN
                     PSIMDKLGASYDAVIWVTSAYLLGFAVPLLLAGRLGDRYGPKNLYLVGLLVFTAASLW
                     CGLSGTIGMLIAARVVQGVGAALLTPQTLSTITRIFPAERRGVAMSVWGATAGVATLV
                     GPLAGGVLVDGLGWQWIFFVNVPVGIIGLVLAFRLVPELPTEQHGFDIIGVLLSGIGM
                     FMVVFALQEGQSHDWAPWIWGTLAGGVGFMAAFVFWQQANRNEPLVPLGIFRDRDFSL
                     STLGVATIGFVVTAMILPVMFYAQAVCGLSPTRAAVLTAPMAIATGLLAPWVGKIVDR
                     YHPRPIIGFGFSVLAVALTWLSVEMTSTTPIWRIVLPLAATGVGMAFIWSPLAATATR
                     NLASDVAGAGSGVYNATRQVGSVLGSAGMAAFMTTRISAEMPTAGDGPGGEGSVTQLP
                     GFLHEPFAAAMSQSLLLPAFVALFGVVAAMFLLGFASPAAPEPQRSDVADHLQDDADY
                     VDDDDYVEFTVKHDEPLAAAAPAVAQVAPAQVEDEGDTQPIAARQENHLTPAADTRHD
                     PHPAPVEPVVPPVEQVEAPGYRRGPGRSLLDFLADVPPPPPSVEPIGFAHNGFHTDDE
                     QRFQPLSRFEISQEPPPHAEPDSQKPDVLRPDYPLDFGSHAHYESPDEPGFAPRHELT
                     DDEPQANGRHSRAEPGDTSSRRGRHSRPGE"
BASE COUNT          425 a          877 c          874 g          358 t
ORIGIN      
        1 tcccggtgct gttcggccac atcggcgagg gcaacctgca cctcaacgtg gtgcgctgcg
       61 cgctggacgg ggagcgggag gcccgactgt attcggcgat gatggcgttg atcgcccggt
      121 gcggaggcaa cgtcagctcg gaacacggtg tcggcagccg caaacgcgac tacgttccca
      181 tgtcgcgaac cgtggccgac atcgctgcga tgcggttggt caagaccgcg ttcgaccctg
      241 gcggctacct caacgcggcc gtgctcttcg actagtgggt accgacctga actactgcga
      301 cgcctgaggc cggcgggtgg atccggctat tcgcccggcc gggagtgccg cccacggcgg
      361 ctcgatgtgt cgccgggttc ggcgcgggaa tgcctgccgt tggcctgcgg ctcgtcgtcg
      421 gtcaactcgt ggcgcggcgc gaagccgggc tcgtcggggc tctcgtaatg ggcgtggctg
      481 ccgaagtcga gcgggtagtc ggggcggaga acgtccggct tctgcgaatc gggctcggca
      541 tgcggcggcg gctcctggct gatctcgaag cggctgagcg gctggaaccg ctgctcgtca
      601 tcggtgtgaa acccgttgtg ggcgaaccca atcggctcca ccgatggtgg cggcggaggt
      661 acatcggcga ggaaatcgag caggctgcgg ccgggcccac gtcgataccc gggcgcctcg
      721 acctgctcca cgggcggcac taccggctct acgggcgctg ggtgggggtc atgccgcgta
      781 tccgcggcag gtgtgaggtg attctcctgc cgcgcggcga tcggctgggt gtccccctcg
      841 tcctcgacct gcgcgggggc gacctgcgcg acagcaggcg cggcggcggc aagcggttcg
      901 tcgtgcttca cggtgaactc gacatagtcg tcgtcgtcga cgtaatcggc gtcgtcctgg
      961 aggtggtcgg ccacgtcgga acgctgcggc tcgggggcgg ccgggctggc gaacccgagc
     1021 aggaacatcg ccgcgaccac accgaacaac gcgacgaacg ccggcagcag caacgactgg
     1081 gacattgcgg ccgcgaacgg ttcgtgcagg aaacccggca gctgggtgac agaaccctcg
     1141 ccgcccggtc cgtcgcccgc cgttggcatc tcggcgctga tccgcgtcgt catgaacgcc
     1201 gccatgcctg cgctgcccag caccgacccc acctgccggg tggcgttgta cacacccgag
     1261 cctgcgcctg cgacatccga tgccaggttg cgcgtcgccg tcgccgccaa cggcgaccag
     1321 atgaacgcca tccccacgcc ggttgcggcc agcggcaaca cgattcgcca gatcggcgtc
     1381 gtcgacgtca tctcgacgga caaccacgtc aacgcgaccg ccaacaccga gaagccgaat
     1441 ccgatgatcg gccgcgggtg gtaccggtcg acgatcttgc ctacccacgg ggccagcaga
     1501 ccggtcgcga tcgccatcgg cgcggtcagc acggccgcgc gggtcggcga aagaccgcac
     1561 accgcctgcg catagaacat caccggcagg atcatcgccg tcacgacgaa gccgatggtg
     1621 gcgacaccga gcgtggacag gctgaagtcg cggtcgcgga agatacccag cggcaccagc
     1681 ggctcgttgc ggttggcctg ctgccagaag acgaacgcgg ccatgaagcc caccccaccg
     1741 gccagcgtgc cccagatcca cggggcccag tcgtgtgact gcccttcctg caaggcgaag
     1801 acgaccatga acatgccgat gccggagagc agcaccccga tgatgtcgaa accgtgctgc
     1861 tcggtgggga gttccggaac cagccggaag gccagcacca acccgatgat tccgaccggc
     1921 acgttgacga agaagatcca ctgccagccg agcccgtcga cgaggacacc gccggccagc
     1981 ggtccgacca gagtggcgac ccccgcggtg gctccccaca cgctcatcgc caccccgcgt
     2041 cgctcggcgg ggaagatccg ggtgatcgtg gacagggtct gcggagtgag caacgcggcg
     2101 ccgacgccct gcaccacccg ggccgcgatg agcatgccga tggtgccgga cagtccgcac
     2161 cacagcgacg cggcggtgaa caccagcagg ccgaccaggt agaggttctt cggcccgtaa
     2221 cggtcgccga ggcggcctgc cagcagcagc ggcaccgcga agccgagcag gtaggcgctg
     2281 gtcacccaga tgacggcgtc gtagctggca cccagcttgt ccatgatgga cgggttggcg
     2341 accgccacga tggtcgcgtc caccaggatc atgaagaagc cgaccatcat ggcccagagc
     2401 gcatgccacg ggtcagcgac cggcgcggcc ccacccgtgg gccggccccg cagcgccgtg
     2461 gtcatatccc cacctcgtct cggcctccct cgatggtgtc ggcttctgac gccccgcgcc
     2521 accgcaaccg cctg
//
LOCUS       DASYLX010000613         8949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2553579,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000613 DASYLX010000000
VERSION     DASYLX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553579"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..862
                     /locus_tag="VGP27_14540"
     CDS             5..862
                     /locus_tag="VGP27_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7852550.1"
                     /translation="MRFAFKTSPQNTAWHDMLAVWQAADDIDVFESGWTFDHFYPIYS
                     DPAGPCLEGWTTLTALAQATKRLRVGVLVTGIHYRHPAVLANMAAALDIVSGGRLELG
                     IGAGWNEEESGAYGIELGSIKERFDRFEEACEVLTSLLSKETTTFDGKYYQLKDARNE
                     PKGPQRPHPPICIGGSGEKRTLPLTAKYADHWNFVGGPPEEFARKRDVLAARCADIGR
                     DPHEITLSAHVRLEPDLDYAKVVHDAGSLGKEGLDLAVIYLPPPHDPRVLEPLAEAIR
                     DSGLTSKFN"
     gene            complement(943..1125)
                     /locus_tag="VGP27_14545"
     CDS             complement(943..1125)
                     /locus_tag="VGP27_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852551.1"
                     /translation="MPQLSNCYTAGVIDVYLNPLSRSRMSRIAWSLLRHPVKCREYPA
                     KAERLVTADELLRFGY"
     gene            complement(1241..2032)
                     /locus_tag="VGP27_14550"
     CDS             complement(1241..2032)
                     /locus_tag="VGP27_14550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV7852552.1"
                     /translation="MSLDRRHFLVALSAVTVGAMGLTRCSTGAQPRVAEATPVAVEQP
                     PALLPPPPRAARIPLPGGGVLSSLPDDGDLLALTVDDGVSSDVVRLYTEFARDTGVRL
                     TYFVNGAYRSWTENQALLRPLVDSGQIQLGNHTWSHPDLTKLPLTAVAEELRHTHDFL
                     WKAYGTDARPYFRPPYGAHNANVDKISGELGYTADTLWSGSLEDHVVIPADEIVKMAD
                     KYFTPGAIVIGHLNHEPVTHVYGRLVELIRERRLRTVTLRDVYVG"
     gene            complement(2092..3558)
                     /locus_tag="VGP27_14555"
     CDS             complement(2092..3558)
                     /locus_tag="VGP27_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEV7852553.1"
                     /translation="MPDPRGFLKYTKRETPHRRPVPLRLRDWKEVYEDFSHDALREQA
                     TRCMDCGIPFCHNGCPLGNLIPEWNDLVRSDRWRDAIERLHATNNFPEFTGRLCPAPC
                     EGSCVLGINQDPVTIKQVEVEIIDNAFGEGWVVPLPPDKLTGKTVAVVGSGPAGLAAA
                     QQLTRAGHSVTVFERDDRIGGLLRYGIPEFKMEKRHIDRRLEQMTAEGTQFRAGVNVG
                     VDITAEQLRSEFDAVVLAGGATAWRDLPIPGRDLDGIHQAMEFLPWANRVQQGDPVTD
                     DDGLPPLHAKGKRVIIIGGGDTGADCLGTVHRQGCESVHQFEIMPRPPESRADSTPWP
                     VYPLMLRVSSAHEEGGERVYSVNTEEFIGRDGKVTGLRGHEVKMAGGKFEKLDGTEFE
                     LEADLVLLAMGFVGPERDGLLNALSVEITDRGNVARDPDFSTSVSGVFVAGDMGRGQS
                     LIVWAIAEGRAAAAGVDRYLMGRTALPAPIKPTAVPQR"
     gene            complement(3551..8185)
                     /gene="gltB"
                     /locus_tag="VGP27_14560"
     CDS             complement(3551..8185)
                     /gene="gltB"
                     /locus_tag="VGP27_14560"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEV7852554.1"
                     /translation="MAPGNQGLSGSSRAQGLYNPAFEHDACGVAMVADMHGRRSRDIV
                     DKAIMALVNLEHRGAQGAEPHTGDGAGILLQVPHAFLRAVVEFELPEEGSYATGIAFL
                     PQSSKDAAAACDSVEKIAQAEGLAVLGWRDVPTDDSSLGALARDAMPTFRQVFMAGAS
                     GMDLERKAYVVRKRAEHELGTKGPGQDGPGRETVYFPSLSGQTFVYKGMLTTPQLKAF
                     YLDLQDDRLTSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREA
                     LIKTDIFGSVPAAGRESGTDLDKVVPICTPGASDTARFDEVLELLHLGGRSLPHAVLM
                     MIPEAWERNESMDPARRAFYRYHASLMEPWDGPASMTFTDGTVIGAVLDRNGLRPSRI
                     WVTQDGLVVMASEAGVLNLDPSTVVKKMRLQPGRMFLVDTAQGRIVDDEEIKAELAAE
                     LPYQEWLDAGLFHLDELPPGDYVRMPHHRVVLRQQIFGYTYEELNLLVAPMARSGAEP
                     LGSMGTDTPVAVLSERPRMLYDYFQQLFAQVTNPPLDAIREEVVTSLAGTVGPEGDLL
                     NPDAESCRQIVLSNPILRNAELSKLMCVDPDHEIRGHKHGMRAAVIRCLYPVNRGGQG
                     LREALDNVRAKVSEAIRDGARIIVLSDRESDESMAPIPSLLSVSAVHHHLVRDRTRTK
                     VGLVVEAGDAREVHHMACLVGFGAAAINPYMAFESIEDMCDRGVITGINSDKAKANYV
                     KAAGKGVLKVMSKMGISTLASYTGAQLFQAIGISQQLLDEYFTGLTCPVGGIDLDDIA
                     DDVTRRHSLAYLDRPDEWAHRELEVGGEYQWRREGEYHLFNPDTVFKLQHSTRTGQYS
                     IFKEYTALVDDQSERMASLRGLLKFRDGVRQPVPLDEVEPAGEIVKRFSTGAMSYGSI
                     SAEAHETLAIAMNRLGGRSNSGEGGEDVKRFEHDQNGDWRRSGIKQVASARFGVTSHY
                     LTNCTDIQIKMAQGAKPGEGGQLPGNKVYPWIAEVRHSTPGVGLISPPPHHDIYSIED
                     LAQLIHDLKNANPQARVHVKLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTS
                     QKHAGAPWELGLAETQQTLLLNGLRDRIVVQVDGQLKTGRDVVIAALLGAEEFGFATA
                     PLVVSGCIMMRVCHLDTCPVGVATQNPVLRQRFDGKPEFVENFFLFIAEEVREMMAQL
                     GFRTVNEMVGQVGALDTTKAAEHWKAYKLDLAPVLHEPESAFMNQDLYCSSRQDHGLD
                     KALDQQLIVMCREALDSGTPVRFATTIANTNRTVGTMLGHELTKAYGGQGLPDGTIDI
                     TFDGSAGNSFGAFVPKGITLRVYGDANDYVGKGLSGGRLVVRPSDDAPEDYVAEENII
                     AGNVILFGATSGQAFLRGVVGERFAVRNSGAHAVVEGVGDHGCEYMTGGKVVILGATG
                     RNFAAGMSGGVAYVYNADGNFAENLNAEMVDLDEMDDEDVDFLRSMITAHVDATDSAV
                     GQRIMNDWDNQVRNFVKVMPRDFKRVLQAIAEAEKSGEDVDTAIMAAANA"
     gene            complement(8649..8909)
                     /locus_tag="VGP27_14565"
     CDS             complement(8649..8909)
                     /locus_tag="VGP27_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7852555.1"
                     /translation="MPVLTDNQIGACTQDPDRWMTATDDQTKAVCRSCPRRWLCAKEA
                     CEMPGAQGLWAGIHIPEGGRGRTFALKQLRSLAERGGQTVHR"
BASE COUNT         1491 a         2999 c         2892 g         1567 t
ORIGIN      
        1 tgtagtgcga ttcgccttca agacatcccc gcagaacacc gcttggcacg acatgcttgc
       61 cgtctggcag gccgccgacg acatcgacgt cttcgaatcc ggctggacgt tcgaccactt
      121 ctatccgatt tactccgacc cggccggccc gtgcctcgag ggctggacga cgctgaccgc
      181 gctggcgcag gccaccaagc gtctccgcgt cggcgtactg gtaaccggca tccactaccg
      241 ccatcccgca gtgctggcca atatggccgc ggcgctggac atcgtgtccg gcggccggct
      301 cgagttgggc atcggcgcgg gctggaacga agaggaatcc ggcgcgtacg ggatcgaact
      361 cggctccatc aaggaacgct tcgaccgctt cgaggaggcg tgcgaggtgc tcaccagctt
      421 gttgagcaag gagacaacga ctttcgacgg caagtactac caactcaagg atgcgcgcaa
      481 cgagcccaag ggtccgcagc ggccacaccc gccgatctgc atcggcggca gcggcgagaa
      541 gcgcaccctg ccgctcaccg cgaaatacgc cgaccactgg aatttcgtcg gcggaccacc
      601 tgaggagttc gcacgcaagc gcgacgtgct cgccgcccga tgcgccgaca tcggccgcga
      661 cccgcatgag atcaccctgt ctgcgcacgt ccggctggaa cccgatctcg attacgccaa
      721 ggtcgttcac gacgcgggtt cgctcggtaa ggaagggttg gacctcgcgg tcatctatct
      781 gccgccgccg cacgaccccc gcgtgctcga accgttggcc gaggcgatca gggattccgg
      841 gctgaccagc aaattcaact gacgataacg aaaatataac gttccggcaa agaatcggtg
      901 acggaacgac gtcacgtcgt cttttaggcg gtgtgggggt tgctagtagc cgaagcgaag
      961 aagctcgtcg gcggtgacca ggcgctcagc cttggccggg tattcacggc acttcaccgg
     1021 gtgtcggaga agtgaccagg cgattcgact catccgggaa cgggacagcg ggttgaggta
     1081 cacgtcgatc actcctgcgg tatagcaatt gctgagctga ggcactacgt taccgcagcg
     1141 tcctcaccaa gtcattagat acccgacaac tggcaaacag ttttaggcgc gccgatcaat
     1201 catcagatga ttttattgat gcagaagtga cagatgttgt ttagcccacg tacacgtcgc
     1261 gcagggtgac ggtcctcagc cgacgctcgc ggatgagctc gacgaggcgt ccatagacgt
     1321 gcgtgaccgg ctcgtggttg agatgcccga tcacgatcgc gcccggcgtg aagtacttgt
     1381 cggccatctt cacgatctcg tccgccggga taaccacgtg gtcctccaac gagcccgacc
     1441 agagcgtgtc cgcggtgtat ccaagttcgc cggagatctt gtcgacgttc gcgttgtgcg
     1501 ccccgtacgg cggccggaag tagggccgcg cgtcggtgcc gtaggccttc cagaggaagt
     1561 cgtgcgtatg ccgaagttct tctgccacag cggttaacgg caacttcgtc aggtccggat
     1621 gcgaccaggt gtggtttccg agctggatct gcccgctgtc gacgagcggc cgcaggagcg
     1681 cctggttctc ggtccacgag cggtaggccc cgttgacgaa gtacgtcagc cggaccccgg
     1741 tgtcccgcgc gaattcggtg tagagccgca ccacgtcgct gctcacgccg tcgtcgacgg
     1801 tcagcgccag caggtcgccg tcatcgggca ggctgctgag cacaccgccg cccggcagcg
     1861 ggatccgggc cgcccgtggc ggaggcggca gaagcgcggg cggttgttcg acagcgaccg
     1921 gcgtggcttc ggcgacccgc ggttgggcgc ccgtgctaca cctggtcagg cccatcgccc
     1981 cgaccgtcac agcggatagc gcaaccagga agtggcgccg atccagagac atggccacaa
     2041 ctgtcccgcc cgcggtctca aataccccgc ttttgcaggc gtgtcgcggg tctagcgctg
     2101 aggaacggcc gtgggcttga tcggcgcggg tagcgcggtc cggcccatga ggtatcggtc
     2161 gacgcccgcc gcggcggccc ggccctcggc gatcgcccac acgatcagcg actgtccccg
     2221 gcccatgtcg cccgcgacga agacgccgga caccgacgtg gagaagtccg gatccctcgc
     2281 gacgttgcca cgatcggtga tctcgacgct cagggcgttg aggaggccgt cccgctccgg
     2341 accgacgaag cccatcgcga gcaacaccag gtcggcctcc aactcgaatt ccgtgccgtc
     2401 gagcttctcg aacttgccgc ccgccatctt cacctcgtgg ccgcgcagac ccgtgacctt
     2461 gccgtcgcgg ccgatgaact cctcggtgtt caccgagtac acccgctcgc cgccctcctc
     2521 gtgggcggac gacacccgca gcatcagcgg gtagaccggc cacggcgtgg agtcggcacg
     2581 cgactcagga ggccggggca tgatctcgaa ctggtgcacg ctttcgcagc cttggcggtg
     2641 cacggtgccg aggcagtctg caccggtgtc gccaccgccg atgatgatga ccctcttgcc
     2701 ttttgcgtgc agcggcggca gtccgtcgtc gtcggtcacc gggtcgccct gctgcacccg
     2761 gttggcccac ggcaggaact ccatcgcctg atgtatgccg tccaggtcgc ggccggggat
     2821 cggcagatcc cgccatgccg tcgcgccgcc cgccagcacc accgcgtcga actcggagcg
     2881 caactgctcg gcggtgatgt ccacgccgac attgacgccg gcgcggaact gggtgccttc
     2941 ggccgtcatc tgctccagac gacggtcgat gtggcgcttt tccatcttga actcagggat
     3001 gccgtagcgc agcagtccgc cgatgcgatc atcacgctcg aacaccgtca ccgaatgacc
     3061 ggcgcgggtg agctgctgtg cggcagccag cccggccggg cctgagccga cgacagcgac
     3121 cgtcttgccc gtcagcttgt ccggcggcag tggcacaacc cagccttcac cgaacgcgtt
     3181 gtcgatgatc tcgacctcga cctgcttgat ggtgacagga tcctggttga tgcccagcac
     3241 gcaggatccc tcacacggcg cgggacacag ccggccggtg aactccggga agttgttggt
     3301 ggcgtgcagc cgctcgatcg catcgcgcca gcggtcgctg cgtaccagat cattccactc
     3361 agggatcaga ttgcctagcg gacacccgtt gtggcagaac gggataccgc agtccatgca
     3421 gcgcgtggcc tgctcgcgca acgcatcgtg ggagaagtcc tcgtagacct ctttccagtc
     3481 acgcagacgc agcgggaccg gccggcggtg cggggtctcg cgcttcgtgt acttgagaaa
     3541 gcctctggga tcaggcattc gcggccgcca tgatcgccgt gtcgacatcc tcgccggact
     3601 tctcggcttc ggcgatcgcc tgcagcacgc gcttgaagtc gcgcggcatc accttgacga
     3661 agttgcgcac ctggttgtcc caatcgttca taatgcgttg cccgacggcg gaatccgtgg
     3721 catcgacgtg cgcggtgatc atagagcgca ggaagtcgac gtcctcgtcg tccatctcgt
     3781 cgagatcgac catctcggcg ttgagattct ccgcgaagtt gccgtcagcg ttgtagacgt
     3841 atgccacccc gccggacatc cccgccgcga agttgcggcc ggtggcaccg aggatgacca
     3901 ccttgccgcc ggtcatgtac tcgcagccgt ggtcgccgac gccctccacc accgcgtgcg
     3961 cgccggagtt gcgcaccgcg aaccgctcgc cgacgacacc gcgcaggaac gcctgcccgc
     4021 tcgtcgcacc gaacaggatc acgttgcccg cgatgatgtt ctcctcggcg acatagtctt
     4081 cgggggcgtc gtccgacgga cgcaccacca gccgaccacc ggacaggccc ttgccgacgt
     4141 agtcgttggc gtcgccgtac acccgcagcg tgatgccctt cggtacgaac gcaccgaagc
     4201 tgttgcccgc cgatccgtcg aacgtgatgt cgatggttcc atcgggaagg ccctgaccgc
     4261 cataggcttt ggtgagctca tggccgagca tggtgccgac ggtgcggttg gtgttggcga
     4321 tggtcgtcgc gaaccggacc ggggtgccgg agtccagcgc ctcccggcac atcacgatga
     4381 gctgctggtc gagcgccttg tcgaggccgt gatcctgacg cgaactgcag tacaggtcct
     4441 gattcatgaa cgcggattcc ggctcgtgca gcacaggggc caagtcgagt ttgtaggcct
     4501 tccagtgctc ggcggccttc gtggtgtcca gcgcgccgac ctggccgacc atctcgttga
     4561 cggtgcggaa gcctaactgc gccatcattt cccgcacttc ctcggcgatg aacaagaaga
     4621 agttctccac gaactccggc ttgccgtcga accgctgccg cagtacgggg ttctgggtcg
     4681 ccaccccgac cggacaggtg tcgagatgac acacccgcat catgatgcag ccggacacca
     4741 ccagcggggc ggtggcgaag ccgaactcct cggcgccgag tagcgcggcg attaccacgt
     4801 cgcgaccggt cttgagttgc ccgtcgacct ggaccacgat gcggtcgcgt agcccgttga
     4861 gcagcaacgt ctgctgcgtc tcggccagtc ccagctccca cggcgcgccg gcgtgtttct
     4921 gcgacgtcag cggcgtcgcg ccggtgccgc cgtcgtggcc ggagatcagc acgacgtcgg
     4981 cgtgcgcctt ggacaccccg gcggccacgg tgccgacgcc gttctcgctg accagcttga
     5041 cgtgcacgcg ggcctgcggg ttggcgttct tcaaatcgtg gatcagctgc gccagatcct
     5101 cgatggagta gatgtcgtgg tgcggcggcg gcgagatcag gccgacaccc ggcgtggaat
     5161 gccgaacctc ggcaatccac gggtacacct tgttgcccgg aagctggccg ccctcaccgg
     5221 gtttcgcgcc ctgggccatc ttgatctgga tatcggtgca attggtcagg taatgcgacg
     5281 tgacgccgaa acgcgccgag gcgacctgct tgatgccgct gcggcgccag tcgccattct
     5341 ggtcgtgctc gaagcgcttg acgtcctcgc cgccctcgcc cgagttcgac cggccgccaa
     5401 ggcgattcat cgcgatggcc agcgtctcat gcgcctcggc ggagatcgag ccgtaactca
     5461 tcgcgccggt ggagaaccgc ttgacgatct cgccggccgg ctccacctcg tcgagtggca
     5521 ccggttggcg tacaccgtcg cggaacttca gcaggccgcg cagcgacgcc atccgctcgc
     5581 tctggtcgtc gaccagtgcc gtgtactcct tgaagatcga gtactggccg gtgcgggtgg
     5641 agtgctgcag cttgaacacc gtgtccgggt tgaacaggtg atactcaccc tcgcggcgcc
     5701 actggtactc gccgcctacc tcgagctcgc ggtgggccca ctcgtcgggc cggtccaggt
     5761 atgccaacga gtgccgccgg gtgacgtcgt cggcgatgtc gtcgaggtcg atgcccccga
     5821 cggggcacgt caacccggtg aagtactcgt cgaggagctg ctggctgatg ccgatggcct
     5881 ggaacagctg cgcacccgtg taggacgcaa gcgtcgagat gcccatcttg gacatcacct
     5941 tcagcacacc cttgcctgcg gccttgacgt agttggcctt ggccttgtcg ctattgatgc
     6001 cggtgatcac gccacggtcg cacatgtcct cgatcgactc gaacgccatg taggggttga
     6061 tcgcggcggc gccgaaaccg accaggcacg ccatgtggtg cacctcgcgc gcgtcgcccg
     6121 cctccaccac gagcccgacc tttgtgcggg tgcggtcccg aaccaggtgg tggtgcacgg
     6181 ccgagacgct gagcagcgac gggatcggcg ccatcgactc gtcggactca cggtcggaca
     6241 atacgatgat gcgcgcgccg tcccggatcg cctccgacac cttggcgcgg acgttgtcga
     6301 gtgcctcccg cagtccctga ccgccgcggt tcaccggata gagacagcgg atcaccgccg
     6361 cgcgcatccc gtgcttgtgg ccgcggatct cgtggtcggg atcgacgcac atcaacttcg
     6421 acagctcggc gttgcgcagg atcgggttgg aaagcacgat ctgccgacac gactcggcgt
     6481 cggggttgag gaggtcgcct tcgggtccga cggtgcctgc caggctggta accacctctt
     6541 cgcggatcgc atccaagggg gggttggtga cctgggcgaa cagctgctga aaatagtcgt
     6601 agagcatccg cggccgctcc gacagcacgg cgaccggggt gtcggtgccc atcgaaccga
     6661 gcggttcggc tccgctgcgc gccatcggcg ccaccaagag gttgagctcc tcgtaggtgt
     6721 agccgaagat ctgctggcgc aacacaaccc ggtggtgcgg catgcggacg tagtcgccgg
     6781 gcggaagctc atcgaggtgg aacaggccgg catcgagcca ctcctgatac gggagctcgg
     6841 cggcgagttc ggccttgatc tcctcgtcgt cgacgatgcg gccctgcgcg gtgtcgacga
     6901 ggaacatcct gccgggttgc agacgcatct tcttcacgac ggtcgacggg tcgaggttca
     6961 gcacgcctgc ctccgacgcc atcaccacca ggccgtcctg ggtgacccag atccgcgaag
     7021 ggcggaggcc gttgcggtcc agcaccgcgc cgatcactgt tccgtcggtg aacgtcatcg
     7081 acgccgggcc gtcccacggc tccatcaacg atgcgtggta ccggtagaac gcccggcgcg
     7141 ccgggtccat cgactcgttg cgttcccacg cctccgggat catcatcagc acggcgtgcg
     7201 gcaggctgcg tccgccgaga tgcagcaact cgagcacctc gtcgaagcgc gcggtgtccg
     7261 atgcgccggg cgtgcagatc ggcaccacct tgtcgagatc tgttcctgat tcgcggcccg
     7321 ccgcgggcac ggagccgaag atgtcggtct tgatcagcgc ttcacgcgcc cgcatccagt
     7381 tctcgttgcc ggtgacggtg ttgatctcgc cgttgtgtgc gatccggcgg aacgggtggg
     7441 ccagcggcca cgacgggaac gtgttcgtcg agaacctcga gtgcacgatg cccagcgcac
     7501 tggtcagccg gtcgtcctgc aggtcgagat agaacgcctt cagctgcggc gtggtcagca
     7561 tgcccttgta gacgaatgtc tgaccggaaa ggcttgggaa gtagaccgtt tcgcggccgg
     7621 gaccgtcctg gcccggcccc ttcgtcccga gttcgtgctc ggcgcgcttg cgcaccacgt
     7681 acgccttgcg ctcgagatcc atgccggagg caccggccat gaacacctga cggaacgtcg
     7741 gcatggcgtc gcgggccaga gcgccgagcg acgagtcgtc ggtgggcaca tcccgccagc
     7801 ccagcacagc caagccctcg gcctgcgcga tcttctcgac ggaatcgcag gccgccgcgg
     7861 catccttgga cgactgcggc aggaatgcga tgccggtggc gtagctgccc tcctcgggca
     7921 gctcgaattc gacgacggct cgcaggaacg cgtgggggac ctgcaacagg atgcccgcgc
     7981 cgtcaccggt gtgcggttcc gcgccctggg caccgcggtg ctccagattg accagggcca
     8041 tgatggcctt gtcgacgatg tcgcggctgc ggcggccgtg catgtctgcg accatggcga
     8101 cgccacacgc gtcatgttcg aacgcggggt tgtagagccc ctgcgcacgc gaggagccgg
     8161 acaacccctg gttaccgggc gccattccca cctagccctt caagactcac tgaaacaatg
     8221 ttggccactg acgacggcgt cagccgatca tggcggtggc ggtgcctgcg tgcagcactg
     8281 aacgacgacc cctaccccac ttggcacaag tgggaaaacg atatgacaaa ccccggttga
     8341 catgccaact tagtagcacc taactaggtc gattggcccc cacggcaacg ctgccccatt
     8401 cgctgccaat gatacgagcg atttcgttgc acgccttcga ttttcagtcg cccaagctgc
     8461 ggccgtcgcc gcggttcgct gcgcccgggt gcgatcaaaa tcactagcgt gaattgctgg
     8521 cggcgggtca gaattgtcag gaagtgggta aagccgacta caccacgttt gctataaagc
     8581 ctagccagat tcttagattc cggccgcgcc gcccgcccgc tcccctcacc gggcgggccg
     8641 cgggtcgatc agcggtgcac cgtctgtccg ccgcgctcgg ccagcgaccg cagctgcttc
     8701 agggcgaagg tgcggccgcg gccaccctcc gggatgtgga taccggccca cagtccctgg
     8761 gcgcccggca tctcgcaggc ctccttcgcg cacagccagc ggcgcggaca ggatcggcat
     8821 accgccttgg tctggtcatc ggttgcggtc atccaccggt cggggtcctg cgtgcacgcg
     8881 ccgatctggt tgtcggtcaa cacgggcatc tcgatcatga cggtcatggc cgcttcctct
     8941 cgctcggtg
//
LOCUS       DASYLX010000614         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3541207,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000614 DASYLX010000000
VERSION     DASYLX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541207"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGP27_14570"
     CDS             complement(<1..253)
                     /locus_tag="VGP27_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7852556.1"
                     /translation="MTSAEDGKLKRNITGPLLFLFILGDVLGAGIYALMGVLSEDVGG
                     VLWAPLVTALLLALLTAGSYAELVTKYPKAGGAAVFAERA"
     gene            880..1083
                     /locus_tag="VGP27_14575"
     CDS             880..1083
                     /locus_tag="VGP27_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852557.1"
                     /translation="MGLTIAPTVPIPQWRDGYRDGRDGHPQVRQYGSAKYVYPRETMT
                     ELRLWFESELATTLPEARTLYRT"
     gene            complement(1088..2056)
                     /locus_tag="VGP27_14580"
     CDS             complement(1088..2056)
                     /locus_tag="VGP27_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852558.1"
                     /translation="MTKTRTRPSTLSATLEAELSPFTGPGSPMLVGLLDLRHRVAVER
                     TAASVDELEVRNLSLAKAYAFASVDFPGAAWSFALDTNGVTTVGYFVFDPSVNPVQAT
                     AFTFSGGAYQTLSVPNATTSYGAGINSSGSIVGFYQNLSGKILGYVKSGGTFSDVEYP
                     GAAITQAVDINDSGIVVGAYSDAAGKQHGFRYGSGASTAIDFPGASGTAATGINVDGD
                     IVGFYYDATATHGFTLKGDVFTPIDFPLAGLTAAWGINSAGDISGYYMDGAGGPMHGF
                     LFAGGAFSTIDVAGARQTQLARINDTAHVTGVYIDALTGQHGFLGT"
     gene            complement(2131..3510)
                     /locus_tag="VGP27_14585"
     CDS             complement(2131..3510)
                     /locus_tag="VGP27_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852559.1"
                     /translation="MGSPASVSAASSVLERLPISRWHWRLVVLVGIGTFFDLYEVFLG
                     GVLAPVLAKEWGLGTLGKSMVIAAGFAGMFIGANVLSVAADRLGRRRVFILNLLVYSL
                     FSFAAAFSPNIETFLVLRVLAGIGLGAELVLVDTYLAEFIPAPYRGRMTAWAYTIGFL
                     GVPLAALLGGRLVARQELLGIEGWRWLLLFGGLGAVFIMAVRTMLPESPRWLESRGRH
                     EEARRVVESVAAKVAPDTPVAESAVPDGPSASGDDAPSVAEVLRIAFRDYRKRTVMLV
                     IFQILQTVAYYGFGTLAPLVLVGKGFEVTESLGYAALAFAGYPIGSLLSVPLVERFER
                     KHLIIVSAVGIAVCGIVFAASTNVAVIVVAGFLLTAVSNVFSNGFHIYQAEIFPTAIR
                     STASGICYSLSRATSTVMPFVAVPALAALGPVAVFSGSAVLIVLLCLDVGFLGPRSTG
                     LQLEQLQQR"
     gene            complement(3605..3808)
                     /locus_tag="VGP27_14590"
     CDS             complement(3605..3808)
                     /locus_tag="VGP27_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852560.1"
                     /translation="MMLIAGFLFVIRRRVGVVMARSTSTRSGYRSQEPLRVWRRKWAV
                     AAAASAAGNSVNPSVGLAAPIDG"
     gene            3798..4061
                     /locus_tag="VGP27_14595"
     CDS             3798..4061
                     /locus_tag="VGP27_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852561.1"
                     /translation="MSIIPTRVQDQVNRIDRTRSLWIGISAGVTALWSLYRVFWLFYA
                     ATALSSVGYTGISLIFPLVLWGAIAVVAGFVSVTFQLRYSKQA"
BASE COUNT          841 a         1408 c         1354 g          714 t
ORIGIN      
        1 aggcgcgttc ggcgaagacc gccgcgccac ctgccttcgg gtacttggtg accagttcgg
       61 cgtaggaccc ggctgtcagc agcgccagca gcaacgccgt caccagcggc gcccagagca
      121 cgcctccgac atcctcggac agaaccccca tcagtgcgta gatgccggcg ccgaggacgt
      181 ctccgaggat gaagaggaac aacagcggac cggtgatatt tcgtttgagc tttccgtcct
      241 cagctgatgt catgcacccc tgatacccag tgaggggatg gaagtactcc tggcggcgaa
      301 atccgcgcct cgtgcggcac ggtgtcgctc agggttatcg ggtccacgct cgactgtcgt
      361 tgagagcgaa acgtcttcgc gcggacgtcc tcctggtcgt gcagaactct cgcacgatgg
      421 catgcgaccg ccgcggcgtg aaccagattg ttggcggtaa atcgaacgag ctcagaggca
      481 atgcactcgt cctctaccgc agagatcagt tggtcggact tagcgacaat cagccgccag
      541 gagtcgatgt caatcgcccg atgctcctcc cgggaggcgg cgaacaagct cagtgacgct
      601 gctgatttca acgtactccg gctacccgac cttgacgcct tcaacgggat gaaatgcagc
      661 ctctcccagc ccgtcgcgac tcgaacgcta cctaaagact cgacactcag cggcaaagaa
      721 ctgaattctt cgcaaactga cagacctgtc cacgaattgc ttcgttttgt ggcctgtgcc
      781 gaacggcgga agtgaattcg gcctcaatcc tgggggcccg acgccgagcc ggaatgccag
      841 gacgtcacca gcgtttaccc tcgatgcgac ggttatcgtg tggggctgac gatcgcgccg
      901 accgtgccga tacctcagtg gcgcgacggc taccgagatg gacgagacgg ccacccgcaa
      961 gttcggcaat acggctccgc caagtacgtc tacccgaggg agaccatgac cgaactgcgt
     1021 ttgtggttcg aaagcgaatt ggccacaacg cttcccgagg cgcgcacgct gtaccggacg
     1081 tagtacctca cgttccgagg aagccgtgct gtccggtgag cgcgtcgata tagacgcccg
     1141 tcacgtgcgc ggtgtcgttg atccgcgcca gctgagtttg tcgagcgcct gcgacgtcga
     1201 tggtgctgaa cgctcctcct gcgaagagga aaccgtgcat cggtcctccc gctccatcca
     1261 tgtagtagcc ggagatgtcg cccgcactgt tgatgcccca tgcggccgtc aagccggcaa
     1321 gcgggaagtc gatcggggtg aagacgtcgc ctttcagcgt gaacccgtgg gtagccgtgg
     1381 cgtcgtagta gaagccgacg atgtcgccgt ccacgttgat accggtggcg gcggtcccgg
     1441 acgcgccggg aaagtcgata gctgtggaag cgccactgcc gtagcggaac ccgtgctgct
     1501 tgccggcagc gtcggagtag gcaccaacga cgatgccgga gtcgttgatg tcgacggcct
     1561 gagttattgc ggcccccgga tattcgacat cgctgaaggt gccgccactc ttcacatagc
     1621 caagaatttt tccggaaagg ttctggtaga agccgacgat cgaacccgac gagttgatgc
     1681 ccgccccgta gctcgtggtc gcgttcggca cgctgagggt ctggtaggcc ccacccgaga
     1741 acgtgaacgc cgttgcctgc actgggttca cgctcggatc gaagacgaaa tagccgacgg
     1801 ttgtcactcc gttggtgtcc agtgcgaacg accaagccgc gccggggaag tcgacgctcg
     1861 cgaatgcata cgccttggcg agggacaggt ttcgaacttc cagttcgtcg acggacgccg
     1921 cggtacgttc cacagcgact ctgtggcgca gatccagcag gccgaccagc atcggtgagc
     1981 cggggcccgt gaacggtgac agttcggcct ccagcgtcgc gctgagcgtt gaagggcggg
     2041 ttcgtgtttt cgtcatgagc gttgtccttc cactggccga tgaccgcccg aacgtaaggt
     2101 cggcgggcct agcagagcac gcgtagtcgg ctacctctgt tgcaactgtt cgagctgcag
     2161 acccgtactg cgaggcccca ggaagccgac gtcgaggcac agcaacacga tgaggaccgc
     2221 agacccggag aacaccgcca ccggcccgag cgccgccagc gcgggcactg ccacgaacgg
     2281 catcacggtc gacgtcgcac gagacaacga atagcagatt ccgctggctg tgctgcggat
     2341 cgcggtcggg aagatctcgg cctgatagat gtggaagccg ttggagaaga cgttcgacac
     2401 ggcggtcagc aggaagcccg cgacgacgat caccgcgacg ttcgtcgagg ccgcaaagac
     2461 gatgccgcac acggcgattc ccaccgcgga gacgatgatc agatgcttgc gctcgaaccg
     2521 ttcgaccagc ggaaccgaca gcaacgagcc gatcgggtag ccggcgaagg ccagcgccgc
     2581 atagccaagc gattcggtca cctcgaagcc cttgcccacc aacaccagcg gggccagcgt
     2641 gccgaagccg taataggcga ccgtctgcag aatctggaag atcaccagca tcacggtgcg
     2701 cttgcggtag tcgcggaaag cgatccgcag cacctcggcg acgctcgggg cgtcgtcccc
     2761 ggaagccgag gggccgtcgg gcacggccga ttcggccacc ggcgtatcgg gtgcgacctt
     2821 cgcggccacc gactcgacga ctctgcgggc ctcctcgtga cgcccgcggc tctccagcca
     2881 ccgtggtgac tccggcagca tggtgcggac agccatgatg aacactgctc cgaggccgcc
     2941 gaacagcagc aaccaccgcc acccctcgat gccgagcagt tcttgtcgtg cgaccagtct
     3001 gccgccgaga agggcggcga gcggcacccc gagaaagccg atcgtgtagg cccacgccgt
     3061 catccgaccg cggtaggggg ccgggatgaa ctcggccaga taggtgtcga cgagaaccag
     3121 ttccgcgccc agcccgatac cggcgaggac gcgcagcacg aggaacgtct cgatgttcgg
     3181 cgagaaggcg gcggcgaatg aaaatagcga gtacacaagc agattgagga tgaacacccg
     3241 ccggcgtccg agtcgatcgg cggcgacgga cagcacgttg gcgccgatga acatgcctgc
     3301 gaaaccggcg gcgatcacca tcgacttgcc cagcgtgcca aggccccatt ccttcgccag
     3361 taccggtgcc agcacgccgc ctaggaacac ctcgtacagg tcgaagaacg tgccgatgcc
     3421 gaccaggacc accagacgcc agtgccagcg gctgattggg aggcgctcca agaccgacga
     3481 cgccgcgcta accgatgctg gactccccat ctcgcctcct gtggccggtg tcaggcattt
     3541 gcgaacttac tcgccgctcg gccacctccc tttcaagcca cgcacccacg caccgcagac
     3601 acgctcaccc gtcaatggga gccgccagcc cgacgctcgg gttgaccgag ttgccggcgg
     3661 ccgacgccgc cgcggcgacg gcccatttgc ggcgccagac ccgtagcggt tcttgggatc
     3721 ggtagccact gcgagtggat gtacttcggg ccatgacgac gccaacccgg cggcggatga
     3781 cgaaaaggaa cccagcgatg agcatcatcc ccacccgcgt tcaggatcag gtcaaccgca
     3841 tcgaccgcac gcgcagcctc tggatcggga tctccgcagg cgtcaccgcg ctgtggtcgc
     3901 tctaccgagt cttctggttg ttctacgcgg ccacggcgct ctccagcgtc ggctacaccg
     3961 ggatctcgct gatcttcccg ctcgtgctgt ggggcgccat cgccgtcgtc gcgggcttcg
     4021 tgtccgtcac gttccagctc cgctactcca agcaagcctg acacccccga ggacgggtcg
     4081 ctgacgtcag cggccgaact tgaggcggat gcctcatgtg acggaatggt cgcaggggcc
     4141 accctttctg catgcacatt tctggcatcg cctgcaaagc cgcttccccg acaccgtgtc
     4201 ggaccattgg cccgcgggcg aaatgattgg gaggcaacca tgttcaccat cgacgagatc
     4261 gcgacgattc cgctcttctc gacgcttgag gacaaagagc tgaggtacct tgccggc
//
LOCUS       DASYLX010000615         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_251057,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000615 DASYLX010000000
VERSION     DASYLX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251057"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VGP27_14600"
     CDS             complement(<1..372)
                     /locus_tag="VGP27_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEV7852562.1"
                     /translation="MSDTSAHGVVDRATDSKAFEYTARAGFAASGVLHLLVAFIILRL
                     AFGSGGNADQSGALATLAKQPGGAIMLWIATVGLFALGLWRVGEAIGGSKTGERSGRQ
                     QDDSPAWKRVKPLGLAIVNFAI"
     gene            complement(446..988)
                     /locus_tag="VGP27_14605"
     CDS             complement(446..988)
                     /locus_tag="VGP27_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7852563.1"
                     /translation="MHYEDLREVVLVGHSYGGMVITGAADRASDRVGHLVYLDAKAPK
                     DGQSLVDVAGPFMAAARSDSRIVDGVEMCLFPSDETLPFYGVCDPQTLEWMRARLTPH
                     PWRCFEQALRLTNPTALHTIPQSHISTTAFMSLRNVDRLRKISDGRVWDIDTGHDLMI
                     TEPEKVAELLDGVARPFPGR"
     gene            complement(1280..2116)
                     /locus_tag="VGP27_14610"
     CDS             complement(1280..2116)
                     /locus_tag="VGP27_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852564.1"
                     /translation="MSAAVVVAVSLPIIVVIALLIVAVFVSARVHRREPDPASMRRWR
                     GQVQKEEAIALSGCQDRRGKIWRIAAVFAATGALALSGCTSNVNTSEPTTQTATPTAQ
                     EEQEKFVVEPMAEKTVNELPAGPLYWHVENFPALADAEAAAGPLSLATDVEGKVWLFT
                     LGPKGAPTHGGSMVTEIGPLVDVSAPEYLLLISHAVAAPGAKSGVHTHPGTEAFLVLK
                     GQLSQQTPHGVNVVEAGKTLAGGPPEHPMEVTSSGGEELHWLIMFVVDANKPFASDAT
                     FE"
     gene            2227..2490
                     /locus_tag="VGP27_14615"
     CDS             2227..2490
                     /locus_tag="VGP27_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852565.1"
                     /translation="MADVRPESVPLGCTLARCWLNLAQCLLRLSGIGVTEYAREFLGT
                     RVHEKLELDVVGITKDHGWSEPCARCAIAAGVVLAGVVLISFP"
     gene            2575..2943
                     /locus_tag="VGP27_14620"
     CDS             2575..2943
                     /locus_tag="VGP27_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV7852566.1"
                     /translation="MTTWSRAEIGALGEQLAVEHLTSQGLAVLDRNWRCRYGELDVVA
                     ADDATRTAVFVEVKTRTSDRFGGVEQAVTPDKVRRLRRLAGLWLAGQGGSWAAVRIDV
                     IGIRIGRQRTPEIIHLQAVG"
     gene            2943..>3073
                     /locus_tag="VGP27_14625"
     CDS             2943..>3073
                     /locus_tag="VGP27_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7852567.1"
                     /translation="MALGRAFSVAVRGLEGEIVEIEADITSGLPGVHLVGLPDTALQ"
BASE COUNT          526 a          973 c         1010 g          564 t
ORIGIN      
        1 gatcgcgaag ttgacgatgg ccaacccgag cggcttgacg cgcttccacg caggggagtc
       61 gtcctgctgt cgtcctgatc tctctccggt cttggagccg ccgatggcct caccgacgcg
      121 ccacaggccg agggcgaaca ggccgaccgt ggcgatccac agcatgatcg ccccgccggg
      181 ttgcttggcg agtgtggcga gcgcgccgga ctggtcggcg ttgccgccgg atccgaacgc
      241 gaggcgcagg atgatgaagg cgacgagcag atgcaggaca ccgctcgccg cgaagccggc
      301 gcgcgccgtg tactcgaacg ctttgctgtc ggtggctcta tcgactactc cgtgtgctga
      361 cgtgtcgctc atctcgacct cctcgcgggg cccgagtacc cgttttttta cggccgcgaa
      421 accaaatcgg gtgtttgggt gggtttcagc ggccggggaa aggccgggcg acgccgtcga
      481 gcaactcggc gaccttctcc ggctcggtga tcatcaggtc atggccggtg tcgatgtccc
      541 acactcgacc atccgatatc ttgcgcagcc ggtcgacgtt gcgcagcgac atgaacgctg
      601 tcgtactgat gtgagattgt ggaatcgtat gcagggcagt cggattcgtc agacggaggg
      661 cttgctcgaa gcatctccag ggatgcggtg tcaggcgcgc acgcatccac tccagcgtct
      721 gtggatcaca gacgccgtag aacggcagcg tctcatcgct ggggaacagg cacatctcca
      781 cgccgtcgac gattcggctg tccgagcgcg cggcggccat gaacgggcct gcgacatcga
      841 ccaacgactg gccgtctttg ggggccttgg cgtcgagata gaccaggtgt ccgacgcgat
      901 cggaagcacg gtcggcggcc ccggtgatga ccatcccgcc atagctgtga ccgaccagaa
      961 cgacctctcg caggtcttcg tagtgcaagg agcgccgtga tgtccctgat gtggcagtcg
     1021 aggtcgacgc ctgcacacca tcgcatatct tccggcccga ggtttcggtg ctcacgtacg
     1081 ttcttcagcg gtcggggcgt gaccaggact cggtgaatta cgctcccggc ggttaccggg
     1141 atgtcaattc atcgataagg gtgcggaacg tgattcgtat tcttctggtt gcggcggcaa
     1201 tcgatgccgc tgctgtcggt gcggccccca gtgacggtcg ctgacgagcg gtacccgaac
     1261 tgcaaaagcg gcagccgcct cactcgaacg tagcgtccga cgcgaatggc ttgttcgcat
     1321 ccacgacgaa catgatcagc caatgcagtt cttccccgcc gctgctggtg acctccatcg
     1381 gatgctcggg gggccccccc gccagcgtct tgccggcttc gacaacgttc accccgtgcg
     1441 gggtctgttg gcttaactga cccttcagca ccaagaacgc ctcggtgccg ggatgcgtgt
     1501 gcaccccgga cttggcgcct ggcgccgcga cggcatggct gatgagcagc aggtactcgg
     1561 gggcggaaac gtccaccaag gggccgatct cggtgaccat gctgccacca tgcgtcggcg
     1621 ctcccttggg accaagcgta aagagccaaa ccttgccctc cacgtcggtg gccagagaca
     1681 acggaccagc cgccgcctcg gcgtccgcga gcgcggggaa attctcgacg tgccaataca
     1741 gcgggccggc aggcaactcg ttgactgtct tctccgccat cggttctacg acaaacttct
     1801 cttgctcctc ttgcgccgtt ggtgttgccg tctgggtcgt gggttcgctc gtattcacgt
     1861 tgctcgtgca ccccgacaac gccagagcgc cggtcgcagc gaataccgct gcgatgcgcc
     1921 agattttccc ccggcggtct tgacatccgc ttagggcgat tgcctcttct ttctgcactt
     1981 gaccgcgcca gcggcgcatt gatgccgggt ccggttcacg ccgatggaca cgcgccgaca
     2041 cgaacaccgc aacgatcaac agagcgatca ccacgatgat cggcagcgag acggccacga
     2101 caaccgcggc actcatgagc aaaatgtacg ccggtttgca cccgccgcat aggacgacca
     2161 ccccgacgag aaattggtga ccgggaggtc caacggcccg gcgtacgtga cggccaggac
     2221 agcgaaatgg cagatgtgcg tcccgagtca gtcccgctcg gttgcactct cgcccgttgc
     2281 tggctaaacc tggcgcagtg cctgctgcgc ctcagcggaa tcggcgtcac tgagtacgcc
     2341 cgtgagttcc tcggcactcg ggtgcacgag aagctcgagt tggatgttgt cgggatcacg
     2401 aaagaccacg gttggtccga accctgtgcc cgctgcgcga tcgccgcggg cgtggtgctg
     2461 gcgggagtgg tgctgatcag ctttccctga cgactccggt ggatgaagtc tcaactgtgg
     2521 ataaccgacg ccgagctgtg tcggattgtc cgctcgcgcc cgcatcgtta cgccatgacc
     2581 acatggagcc gtgccgagat cggcgcgctg ggagagcaac tggcggtcga gcatctgacg
     2641 tcacaggggc tggctgtgct cgaccgtaac tggcggtgcc gatacggcga gctggatgtc
     2701 gtcgccgccg acgacgcaac ccgcacggcg gttttcgtcg aagtgaagac acgcaccagc
     2761 gatcggttcg ggggagtgga gcaagccgtc acgccggaca aggtgcgccg gctgcgccgg
     2821 ctggcggggt tgtggctggc cgggcagggc ggcagctggg ccgcggtgcg catcgacgtc
     2881 atcggaatcc gcatcggccg tcagcgcacg cccgaaatca tccatctgca ggcggtcggc
     2941 tgatggcgct gggcagagcg ttttcggtgg ctgtgcgtgg gttggaggga gaaatcgtcg
     3001 agatcgaggc cgacatcacc tccggcctgc ccggtgtcca cctcgtaggt ctgcccgaca
     3061 ccgcgttgca gga
//
LOCUS       DASYLX010000616         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3541277,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000616 DASYLX010000000
VERSION     DASYLX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541277"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VGP27_14630"
     CDS             <1..809
                     /locus_tag="VGP27_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TobH protein"
                     /protein_id="HEV7852568.1"
                     /translation="EAPPWIGALDVLVLAGDDSGDPALVSAAATAVRRGARVIVVAPY
                     EGPLRDATAGRAAVLEPRLWVPDDFGLPRYLAAGLAVLHVVDPNLRTDLAALADELDA
                     EALRNSAARELFTNPAKTLADRMSGRAVVLAGDNGATLALARHGGAVMLRVAHRPVAA
                     VCLSDAIVALRAGLGAQSDADREAALFHDDELDGPLPEQVRTVALTLDAERAVVSARV
                     AGLHHVDIVGAEDVPDAGESPVHSGRPEQQLAVLAVRLEMAATYLRLVRG"
     gene            814..2040
                     /gene="manA"
                     /locus_tag="VGP27_14635"
     CDS             814..2040
                     /gene="manA"
                     /locus_tag="VGP27_14635"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV7852569.1"
                     /translation="MNLLRGALRTYAWGSRTAIAEFTGKPSPTAHPEAELWFGAHPGD
                     PAFLETDTGEESLLDAVRSDPEGQLGTAVCERFGSALPFLMKVLAAEEPLSLQAHPSA
                     EQAAEGFAREDRVGIPMSSPIRNYRDRSHKPELLVALDTFEVLAGFRPAPISVELMEA
                     LEVPELDPFINLLAGQPDADGLRALFTTWITAPQPDLDMLVPAVIDGAIDYVRAGNKR
                     FAAEAKTVLELGERYPGDAGVLAALLLNRFTLRSGEGIYLPAGNLHTYLHGVGIEVMA
                     NSDNVLRGGLTPKHVDVPELLRVLDFAPATEASLRPKTVEDGMETVYQTAAPEFSVSV
                     LGIDGERLGHEVDAPSRHDGPQILLCTEGSAVVHAKSSAVTLERGAAAWVSADDGPIR
                     LVAQRPAKLFRATVGI"
     gene            2041..2961
                     /locus_tag="VGP27_14640"
     CDS             2041..2961
                     /locus_tag="VGP27_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7852570.1"
                     /translation="MSAEGSTRAILAALLANAGIAVAKFVGFLITGSSSMLAEAVHSV
                     ADTSNQGLLLLGQRQSRKEADPLHQFGYGRSRYFYSFVVALVLFTLGSVFALYEGSHK
                     ISHPAELTSPIVAIVILVVAIGLEGYSFRTAVKESRPLKRSGSWWRFIRTSRNPELPV
                     VLLEDSGALVGLVFALIGVGLTVLTGNAVWDGIGTVGIGLLLGVIAVVLMVEMHSLLI
                     GEAATGEEDLAIRAALEQTDNIDRVIHLRTQYLSPEELLVGAKIALAPATDLATVATA
                     IDAAEAAIRAAVPAVQVIYLEPDLDRTPTG"
     gene            complement(2918..>3159)
                     /locus_tag="VGP27_14645"
     CDS             complement(2918..>3159)
                     /locus_tag="VGP27_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7852571.1"
                     /translation="MPWWYRPVGGLFDQFLGASETDPLLAEWFLRRFSLLDSLYMVPS
                     MKLVGRTIRHNMALFMAERRASSRSASDRDPVPGR"
BASE COUNT          491 a         1029 c         1115 g          524 t
ORIGIN      
        1 ctgaggcgcc gccctggatc ggcgcccttg acgtgctggt cctcgccggt gacgactccg
       61 gcgacccggc attggtcagc gcggcggcga cggcggtgcg ccgtggtgcc cgggtcatcg
      121 tggtcgctcc ttacgaggga ccgctgcgcg atgccaccgc ggggcgcgca gccgtgttgg
      181 agccgcgact gtgggtgccc gacgacttcg gcctgccgcg ctatctggct gcaggccttg
      241 cggtcctgca tgtggtcgat cccaatctcc gcacggatct cgccgcactg gccgacgaac
      301 tcgatgcgga agcgctgcgc aacagcgccg cacgcgaact gttcaccaat cccgccaaga
      361 cactggccga ccggatgtcg gggcgcgccg tcgtgctcgc aggcgacaac ggggcgacgt
      421 tggcgttggc ccgccacggt ggcgccgtca tgctgcgggt cgcgcaccgg ccggtagccg
      481 ccgtctgcct gtccgacgcg atcgtggcgt tgcgtgccgg gttaggcgcg cagtccgacg
      541 ccgatcgcga ggccgccctg ttccacgacg acgagctcga cgggccgttg cccgagcagg
      601 tccgcaccgt cgcgctgacg ctggacgccg aacgcgcggt ggtgtcggcg cgggtggccg
      661 gactccatca cgtcgacatc gtcggcgccg aggacgtgcc cgacgccggt gagtcaccgg
      721 tgcattcggg tcgtccggag cagcaattgg cggtgttggc cgtccggttg gagatggcgg
      781 ccacctacct gcgactggtt cggggatagc cgagtgaacc tgctgagggg agcactgcgg
      841 acctatgcct gggggtcacg cacagccatc gccgagttca ccggaaagcc aagtccgaca
      901 gcacatccgg aggccgaact ctggttcggc gcgcaccccg gcgacccggc gtttctggag
      961 accgacaccg gggaggagtc gctgcttgac gccgtccgct ccgaccccga gggtcagctg
     1021 gggaccgcgg tatgcgagcg cttcggcagc gcgttgccgt tcctgatgaa ggtgttggcc
     1081 gccgaagaac cgctgtcgct gcaggcacac cccagcgccg agcaggccgc cgagggcttc
     1141 gcgcgcgagg accgggtcgg catcccgatg tcatcgccga tccgcaacta tcgcgaccgc
     1201 agccacaaac cggaacttct ggtggcgctg gacaccttcg aggtgctcgc cgggttccgg
     1261 cccgcgccga tatcggtgga gttgatggag gcgctcgagg ttcccgaact cgatccattc
     1321 atcaatctgc tggccggcca acccgacgcg gacggcctgc gggcgctgtt caccacatgg
     1381 atcaccgcgc cgcaaccgga tctcgatatg ttggtgcccg cggtgatcga cggcgcgatc
     1441 gactacgtcc gtgcggggaa caaacgattc gcggcggaag ccaagacggt gctggaactg
     1501 ggggagcgct acccgggcga cgccggtgta ctggccgccc tgctgttgaa ccggttcacg
     1561 ctgcgctccg gcgagggcat ctatctgccc gccggaaatc tgcataccta cctgcacggc
     1621 gtcgggatcg aagtaatggc gaactcggac aacgtccttc gcggtgggct gaccccgaaa
     1681 cacgtcgacg tgccggagtt gttgcgggtg ctcgacttcg cgcccgccac cgaggcgagc
     1741 ctgcggccga agacggtcga ggatggaatg gaaaccgtgt atcagactgc ggccccggag
     1801 ttctcggtgt cggtgctggg catcgacgga gagcgcctag gtcacgaggt ggatgcgccg
     1861 tcgcgccatg acggcccgca gatcttgttg tgcacagagg gttcggccgt ggtgcacgcc
     1921 aaatccagcg cggtgacttt ggagcggggc gcggcggcgt gggtgtcggc cgacgacgga
     1981 ccgattcggc tggtcgctca gcgtccggcc aagctgttcc gcgcaaccgt gggtatctag
     2041 atgtcggccg aaggcagcac aagggcgatt ctggcggcct tgctcgccaa tgcgggcatt
     2101 gccgtggcga agttcgtcgg cttcctgatc accggcagtt cgtcgatgct ggccgaggcg
     2161 gtgcactcgg tcgccgatac cagcaaccag ggtctgctgt tgttggggca gcgccagtcc
     2221 cgcaaggagg ccgatccgct gcaccagttc ggctacggcc gcagccgtta cttctactcg
     2281 ttcgtggtgg cgctggtcct gttcacgctg ggctcggtgt tcgcgctcta cgagggctct
     2341 cacaagattt cgcacccggc ggagctgacg tcgccgatcg tggcgatcgt gatcctggtc
     2401 gtcgcaattg gcctcgaggg ctacagcttt cgcacggcgg tgaaggaatc gcggccactg
     2461 aagcgctccg gcagctggtg gcggttcatc cgcacgtcac gcaatcccga gctgccggtg
     2521 gtgctgctgg aggactcggg cgcactcgtc ggcctggtct tcgcgctgat cggcgtcggg
     2581 ctgaccgtgc tcacaggaaa tgcggtgtgg gacgggatcg gcaccgtcgg catcgggctg
     2641 ctgctcggcg tcatcgccgt cgtcctgatg gtggaaatgc acagcctgct gatcggcgag
     2701 gccgccaccg gggaagagga tctggcgatt cgggcggcgc tcgagcagac cgacaacatc
     2761 gaccgggtga ttcatctgcg cacccagtac ctgagccccg aggagcttct ggtcggggcc
     2821 aagatcgcgc tcgcaccggc gacggacctg gcgaccgtgg ccaccgccat cgacgcggcg
     2881 gaagcggcga tccgcgcggc ggtgcccgcc gtacaggtca tctacctgga accggatctc
     2941 gatcggacgc cgaccggcta gaggcccgcc tctcggccat gaacagcgcc atgttgtgcc
     3001 ggatggtccg gccgaccaac ttcatcgacg ggaccatata gaggctgtcc agcaagctga
     3061 agcggcgcaa aaaccattcg gcgagaaggg gatcggtctc cgacgccccg aggaactgat
     3121 cgaagaggcc accgacgggg cggtaccacc acggcatcg
//
LOCUS       DASYLX010000617         6756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2884440,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000617 DASYLX010000000
VERSION     DASYLX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884440"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VGP27_14650"
     CDS             complement(<1..125)
                     /locus_tag="VGP27_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7852572.1"
                     /translation="MPAVTADYLQLPRLDVPPLDAIERPVRSVTTAPTGLEGEGFP"
     gene            complement(163..1566)
                     /locus_tag="VGP27_14655"
     CDS             complement(163..1566)
                     /locus_tag="VGP27_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7852573.1"
                     /translation="MLSDVGLTAADCGGHLSFYGLDPIIPSPHRFGAMAAIGLAAKSV
                     AAAALWRARTGEVQDIHVDVRKALRRFCGFFEGRWETMNGRPPAMGGFAGNPFLELPI
                     LHKTRDGRYVVALDFYPRSKTDTLKFLRCTDSIGSIENAILQWRADDLEESAAEEGLI
                     LAKVRTTEEFLTEPQYTEVLSTMPLVTVEKIGDSEPMPFKADGKNPLDGIRAFGMGHV
                     IAGAGMGRDLAMYGADVLNIWRPNATEVEAFAWDVQVGMRSTILDGSREDRAKFDNLL
                     EDADVFFANKRPGYLERNGLTAEELCEKKPGLIHAKVVLHGDTGPWSNRPGFDEIGAA
                     VSGVFCIEGTPSFPKSPVIVPICDNVVGWLGTVGVLEALRRRATEGGSYRVVVSLTRT
                     VLWLLSLGVFDKEYAAATAGSSDEHTYVAPDLFTAETPLGTYQGMTDQAVMSRTKESY
                     RTVFAPRGSSKPQWRQP"
     gene            complement(1674..2651)
                     /locus_tag="VGP27_14660"
     CDS             complement(1674..2651)
                     /locus_tag="VGP27_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV7852574.1"
                     /translation="MSIEKPIRRIAIVGTGVIGASWAAQYLARGLDVVATDPAPNAEA
                     NLRKYVDEAWGQLEEIGLTPGASRERLSFTKDSDQAFAKADFVQENGPERPDFKKKLF
                     AAIDEATPVDSIIASSSSGITPSVIQSACKHPERVLVGHPFNPPHIVPLVEVVGGAKT
                     SPEAIQQAMRFYASIGKKPIHLRKELPGHVANRLQAALYREMLHLIEQDVLSVEEADA
                     AVSYGPGLRWGVMGQSLQWHVGGGAGGINHFMEQLMDPLAAMMQTLGNPSVTPELKQT
                     IVDGVMREAAGRPVDELAREENEVIIGLLRLRADGVGAPSAANHRKQTA"
     gene            complement(2667..3548)
                     /locus_tag="VGP27_14665"
     CDS             complement(2667..3548)
                     /locus_tag="VGP27_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEV7852575.1"
                     /translation="MGRLFLLDLSDNRVLSLNPDGSDRKVIVTECRYPDGIVVDVAAG
                     HIYWTNMGNPKANDGSIERADLDGRNRKTIVAQGRTFTPKQLQLEKKSAKLYWCDREG
                     MRVMRADLDGSNIETIVDMSQGDSRPGLDASKWCVGVAVDVDGGKVYWTQKGPDNAGK
                     GRICRAGIEVPRGQTASNRADIEVLYQALPEPIDLDLDPNNRVMYWTDRGDPPRGNTV
                     NRAPLDPDANGRPAPEIVVTHLMEGIGIALDLKSDRMFITDFGGNVLEAKLDGSDLKA
                     LLVAQGNLTGIAYSEIS"
     gene            complement(3754..5616)
                     /locus_tag="VGP27_14670"
     CDS             complement(3754..5616)
                     /locus_tag="VGP27_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV7852576.1"
                     /translation="MVGALGIVFGDIGTSPIYTVQTIFNPDDPHPVPMTTDNIYGIVS
                     LIFWSVMIIVTLTYVTLVMRADNDGEGGIMALIALVRRWGARGGHTAVVLATLGVFGA
                     ALFAGDSMITPAISVLSAVEGMKVINPALQDWIVPITAVIIIALFAVQRRGTGAVGGL
                     FGPVMIGWFVAIGACGVNGIVNHPKILAALSPVYAVEFMLGHFGIAFFSLAGVVLAVT
                     GAEALCADMGHFGRKAITRAWLFVVLPALVLNYLGQGALLITDKATLSAPFFLLTPGW
                     ARMPMVLLATAATVIASQAVITGAFSLASQAVQLGYLPRLRVVHTSASTYGQVCMPWI
                     NGMLMVAVLILVFAFRSSASLAYAYGMAVTGTITITTILYLYIARKRWRAPLWLTVIG
                     GGALLCVDLLFLAANLTKLVHGAWLPLLIGIATFTVMITWQRGSTIVTRKRETAEGPL
                     RAFVDRLSRRRGLLRVPGTAIFLNRGNKTAPLALRANVEHNHVLHEHVVIVTIDTLPI
                     PRVSDSDRIEIDPLGDTHDGIIYVTVRFGYMETHDVRQALRVLEPTKSEGLIAIAGAS
                     FFLSKIELRKGPEPTMSAWRRNLFIATSRLSTDGAESFSLPRERTLIMGSRIEV"
     gene            complement(5817..>6756)
                     /locus_tag="VGP27_14675"
     CDS             complement(5817..>6756)
                     /locus_tag="VGP27_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7852577.1"
                     /translation="FCVRAAYDDPRALITAPDIDIVSVCVRVPYHRDLVLAALAADKH
                     VYCEWPLGRDLGEAVQMAAAAGTRTVHVAIGLQAHMNPAARRAAELIAAGAIGRPLTS
                     RIYSSTAGFAPRLPAAYAYLNTVESGANLITILGGHTLDLAILVLGGIDSLNALTTVQ
                     HPMVTLTDTGEQIQRTAPDHLLVQARMISGCALAVEVAGNRAPDTRFTFEVVGTEGQI
                     LLAGGHPNGFQAGRLTLSFNGERQWVDEPPHTLPAAAVNVAAMYCALARDISSGENTT
                     PNFDRAVRLTHLIDDVVQSADSGHRLSGKDWPTSDS"
BASE COUNT         1257 a         2096 c         2087 g         1316 t
ORIGIN      
        1 ggaaagcctt cgccttcaag ccccgtcggc gccgtggtca cgctccgcac aggacgctca
       61 attgcatcca gcggcggcac gtcaagccgg ggcaactgta aatagtcggc cgttacagca
      121 ggcatgatca actcccatca ggtggcggtt cataacgcaa gtttacggtt ggcgccattg
      181 gggtttcgat gaaccgcgcg gcgcgaacac cgttcggtat gactccttcg ttcgtgacat
      241 gacggcctgg tccgtcattc cctggtaggt gccaagtgga gtctctgcgg tgaacagatc
      301 gggagcgaca tacgtgtgct catcgctcga tccggcggta gccgcggcgt attccttgtc
      361 gaaaacgcca agcgaaagca gccatagcac ggttcgggtc agagacacga cgactcgata
      421 gctgccgccc tccgtcgcgc gccggcgcag cgcctcgagt acgccgaccg ttccgagcca
      481 acccaccacg ttgtcgcaaa tgggaacaat taccggcgac ttaggaaagc ttggggtccc
      541 ttcgatgcag aacacccccg acacggccgc gccaatctca tcgaagccgg gacggttcga
      601 ccacggccct gtgtcgccgt ggaggaccac cttggcatgg atcagtccgg gcttcttctc
      661 gcacagttct tcggcggtaa gaccgtttcg ctccagataa ccggggcgct tgttggcgaa
      721 gaagacgtcg gcatcctcca gcaggttgtc gaacttggcg cgatcttccc tggagccgtc
      781 caggatggtg gagcgcatcc caacttgtac gtcccaggca aaggcctcga cctcagtggc
      841 gttgggccgc cagatgttaa ggacgtccgc gccgtacatg gcgaggtcgc ggcccatccc
      901 cgcgcccgca atgacgtgcc ccatcccgaa tgcgcggatg ccgtcgagcg gattctttcc
      961 gtccgccttg aacggcatgg gttcgctgtc accgatcttc tccacggtga ccaggggcat
     1021 cgtggagagg acttcggtgt attgcggctc agtgaggaac tcctcggtgg tacgtacctt
     1081 cgccaggatg agaccttcct ccgcggcgga ctcctccaaa tcgtcagcgc gccattgcaa
     1141 tatggcgttc tcaatcgagc ctatgctgtc cgtgcatctc aggaacttga gcgtgtctgt
     1201 cttcgatcga ggatagaagt ccagggcgac gacgtagcgc ccatcccgtg tcttgtggag
     1261 gatcggaagt tcgaggaacg ggttgccggc aaacccgccc atcgcgggag gacgtccgtt
     1321 catcgtctcc catctccctt caaagaaacc gcagaaccgt cggagtgcct tgcgaacgtc
     1381 gacgtggatg tcttgtacct ctccggttct cgcccgccac agggccgcgg ccgccactga
     1441 cttcgcggcc aggccgatcg cggccattgc accgaaccga tgtgggctgg ggatgatcgg
     1501 atccaggccg tagaacgaca ggtgtcctcc gcagtccgcg gcagtcagtc cgacgtcgct
     1561 cagcacctcg ttcgtattcg ccggcagatc gaactccgat gaactcgccg gattcttgag
     1621 tgtcgattcg agtctctcgg tcaaacgtgt ggcgcccgtc gtgaataggg tgctcacgcc
     1681 gtttgctttc gatgattcgc ggctgagggc gcgcccacgc catcggcccg tagccgaaga
     1741 agtccgatga tgacctcgtt ctcttcacga gcgagctcgt cgacagggcg tccggcggct
     1801 tcgcgcatta caccgtcaac gatggtctgc ttgagttcgg gagttacaga cggatttccg
     1861 agtgtctgca tcattgccgc gagcgggtcc atgagctgct ccatgaagtg attgatgccg
     1921 cccgctcccc cgcctacatg ccattgaagg ctctgtccca tcacacccca gcggagtcct
     1981 ggtccatagc taaccgcagc gtcggcctcc tcgacgctca gcacgtcctg ttcgatgaga
     2041 tggagcatct ctcggtacag tgccgcctgc agccggttgg ccacgtgtcc gggcagttcc
     2101 ttgcgcaaat gaatgggctt cttgccgatc gatgcgtaga agcgcatcgc ttgttggatg
     2161 gcctctggcg atgtcttggc gccgccaacc acttcgacga gtgggacgat gtggggcgga
     2221 ttgaatgggt ggccgacgag gaccctttcc ggatgcttgc aagccgattg aatcacgctt
     2281 ggcgttatcc ccgaggagct cgaggcgatg atcgagtcga cgggcgtcgc ctcatcgatg
     2341 gccgcgaaca atttcttttt gaagtcaggc cgctcggggc cgttttcctg gacgaagtcg
     2401 gccttcgcga acgcctgatc cgagtctttg gtgaagctca accgctcgcg cgatgcgccc
     2461 ggcgtcaaac cgatttcttc gagctgcccc caggcctcgt cgacatactt gcgcaagttg
     2521 gcctcagcgt ttggagccgg gtcggttgca acgacgtcta acccgcgggc caaatattga
     2581 gccgcccaac tggcgccgat gactcccgta ccgacgattg caatacgacg aattggtttt
     2641 tcgattgaca tgattacgtc tgcctttcaa gagatttcgg agtatgcgat gccggtcagg
     2701 ttgccctggg caacgagcag ggcctttagg tccgaaccgt cgagcttggc ctcaaggacg
     2761 tttcccccga agtcggtgat gaacattcgg tcactcttga ggtcgagggc gattccgatt
     2821 ccttccatca agtgcgtgac cacgatctct ggggcgggcc ggccgttcgc atccggatcc
     2881 agcggggcgc ggtttaccgt gttgccgcgt ggagggtcgc cccggtcggt ccaatacatg
     2941 acgcggttgt tcggatcgag gtccagatcg atcggctcgg gaagcgcctg atacagaact
     3001 tcgatatccg cgcggttgga cgccgtctgt cctctgggaa cttcgattcc ggcgcggcag
     3061 atgcggccct tgccggcgtt gtcgggaccc ttttgcgtcc agtagacctt gccgccgtcg
     3121 acgtcgacgg ccacgcccac gcaccacttc gatgcgtcca gacccggtcg cgagtcgccc
     3181 tgactcatgt ccactatggt ctcgatattg gagccatcga ggtcggcgcg catcacccgc
     3241 atgccctcgc ggtcgcacca gtacagcttg gcgctcttct tttcgagctg tagctgcttg
     3301 ggagtgaagg tgcggccctg ggcaacgatc gtctttcggt tccgcccgtc gaggtcagcg
     3361 cgctcaatgg agccgtcgtt cgccttgggg ttgcccatat tggtccagta gatgtgtccg
     3421 gcagcaacgt cgaccacgat gccgtcgggg tatctgcact cggtgacgat gaccttgcgg
     3481 tcggagccgt caggattgag cgagaggaca cggttgtcgc tgaggtcgag aaggaacagg
     3541 cgacccacgg tggctgtgtc gccggaccca tgtcgcggtg tccgctgtgg tgaactgatc
     3601 atattctctt tcactccaat gtaatccgtc gagatccaga cccagtgcct gattcacctc
     3661 tgcgccaact aattccatcg tggtgcgagc cgtataagat atcgatgtcg ttggcgacag
     3721 cgatcggcag cgaaggttgt acgcccgcac aggtcaaacc tcgatccgtg accccatgat
     3781 cagagtccgt tctcggggca ggctgaagga ctcggcgccg tcggtgctca gcctggacgt
     3841 ggcgatgaag aggttcctac gccacgccga catcgtcggc tctgggccct ttctcagttc
     3901 gatcttcgac aggaagaacg aagcgccggc gatcgcgatc aatccctcgg acttcgtcgg
     3961 ctcgagtaca cgcaatgctt gccggacgtc atgcgtttcc atgtatccga aacggactgt
     4021 tacgtagatg attccgtcgt gagtgtcgcc gagcgggtcg atctcgatgc ggtcagagtc
     4081 gctgacccgc gggattggca acgtatcgat ggtcacgatc accacgtgct cgtgaagaac
     4141 gtggttgtgt tcgacgtttg cgcgcagtgc caacggtgcg gtcttgttgc cgcggttcag
     4201 gaaaatcgcc gtgccaggta cacgcaatag tcctcggcgc ctcgataatc gatcgacgaa
     4261 tgcccgcagc ggaccttccg cggtctcacg tttgcgggtg actatcgtgc tgccgcgctg
     4321 ccacgtgatc atgaccgtga acgtcgcgat gccgatcaac aacggtagcc acgcgccgtg
     4381 aacgagcttc gtcaggttcg cggccagaaa cagtagatca acacacaaca gtgcaccgcc
     4441 gccaatgaca gtcagccaca gcggggcccg ccatctcttg cgcgctatgt agaggtacag
     4501 aatcgtcgtg atggtgatcg ttccggtcac cgccatcccg tacgcataag ccagtgacgc
     4561 cgaactccgg aacgcgaaca ccagtatcag gactgccacc atcagcatgc cgttgatcca
     4621 tggcatacag acctgaccgt aggtggacgc cgacgtgtgc accacccgta gccgcggcag
     4681 gtacccgagc tgaaccgcct gcgacgccaa cgaaaaagct cccgtgatca ccgcctgcga
     4741 cgcgatgacc gtcgcggcgg tcgccagcaa caccatgggc atgcgcgccc agcccggggt
     4801 gagcagaaag aacggtgcac tcaacgtggc cttatcggta atcaacaacg caccttgccc
     4861 gaggtagttg agaaccagcg ccggcagaac cacgaacaac caagcgcgag tgatcgcctt
     4921 gcggccgaag tggcccatgt cggcacagag cgcctccgcg ccggtgaccg caagcaccac
     4981 cccggccaat gagaagaacg caatcccgaa atgccccagc atgaactcca ccgcatagac
     5041 cggggaaagc gccgccagta tcttcggatg gttgacgatg ccgttcacgc cacaggcccc
     5101 aattgccaca aaccagccga tcatcaccgg cccgaaaaga cctcctacgg caccggttcc
     5161 gcgccgctgc accgcgaata gggcaataat gatgacggcg gtgatcggga cgatccagtc
     5221 ctgcagcgcc ggattgatca ccttcatgcc ctctaccgcg gacagcaccg agatcgcagg
     5281 cgtgatcatg ctgtcaccgg cgaacaaggc ggcaccgaac acgccgagtg tcgcaagcac
     5341 aactgcagtg tgaccgccac gcgcacccca acggcgcacg agcgcgatga gcgccatgat
     5401 gccgccctcg ccgtcgttgt cggcgcgcat caccaacgtg acgtaagtca gcgtcacgat
     5461 gatcatcacc gaccagaaga tcagcgagac gatgccgtag atgttgtccg ttgtcattgg
     5521 cacgggatgc ggatcatccg ggttgaagat cgtctggact gtgtagatcg ggctggtgcc
     5581 gatgtccccg aacacgatgc cgagtgcgcc gaccacgagg gtcacgcgcg ccgtttgcct
     5641 gccgctcggg gcggcaggtg gggaagtcgc ttcagacgtc gtcagcacga tgaggtcccc
     5701 gatcagtctt tccggatttc gttgacctga ttgtttgtcg gtaagccggc cgggtcgatg
     5761 tgcgagacga caacatctct gccacgacgg tgtcgttggg cacggccctt gctgcctcaa
     5821 gagtcgctgg tcggccagtc cttaccggag aggcggtgac cactgtcggc tgattgcacg
     5881 acgtcatcga tgagatgggt caagcgcacg gcgcgatcaa aattaggggt ggtgttttcc
     5941 ccggaggaga tgtcgcgggc cagcgcgcaa tacatcgcgg ccacattgac cgcagcggcc
     6001 ggcagcgtgt ggggcggctc gtcgacccat tgacgctcgc cgttgaagct gagggtgagc
     6061 cgacccgcct ggaagccgtt tgggtgacca cctgccagga ggatttgtcc ctccgtacca
     6121 acgacttcaa aggtgaagcg tgtgtcgggc gcccggttgc cggcgacttc gacagccagt
     6181 gcgcagccgc tgatcatgcg cgcctgtacc agcaggtggt cgggtgccgt gcgttggatc
     6241 tgctcaccgg tgtcggtgag cgtgaccatc gggtgctgca cggtggttag ggcattgagg
     6301 ctgtcgattc cgccgagcac caggatcgcc aggtcaagcg tgtgaccacc taggatggtg
     6361 atcaggttcg cgccgctctc aaccgtgttg aggtaggcgt atgccgccgg taggcgcggc
     6421 gcgaaaccgg cggtgctgga gtagattcgg gaggtcaacg gtctgccaat cgcaccggcg
     6481 gcgatcagct cagccgcgcg gcgcgcggca gggttcatgt gggcctgcag gccgatcgcc
     6541 acgtggaccg tgcgcgtccc cgcggcagcg gccatctgga cggcttcgcc gaggtcacgc
     6601 ccgagcggcc actcacaata gacgtgcttg tccgccgcaa gcgccgccag aaccaggtcg
     6661 cggtgatacg gcaccctcac gcacaccgag acgatgtcga tgtcgggcgc cgtgatcagg
     6721 gcgcgcggat cgtcgtaggc cgcacgcaca cagaac
//
LOCUS       DASYLX010000618         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2335551,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000618 DASYLX010000000
VERSION     DASYLX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335551"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1547)
                     /locus_tag="VGP27_14680"
     CDS             complement(<1..>1547)
                     /locus_tag="VGP27_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV7852578.1"
                     /translation="GTPPAEVFAAVAHEVGHVLGADATTIGRLDPDGAMTVLAVVGDH
                     SDDLAVGSRWTPEPPLALAVALRTGRPARCDDYSHASGAFVDATVRRVGIRSSVATPI
                     TVEGRLWGAIGTGTRREHLPADTEQRLAGFTELIGTAIANAEGRTQLEDSRDELRRLA
                     EEQAALRRVATLVARGLPPAEVFAAVAHEAGSVLDADGTVIVRLDHGPVTTLVASVGA
                     HLAELPVGSRWKPEPPVAVAVALRTCRPARCDNYSQAPGDYADAVRRLGLRSSVAAPI
                     TVEGRLWGAIGVGTRRGRFPADTEHRMAGFTELIGTAIANADSRAQLTASRARIVAAA
                     DDARRRIERDLHDGTQQRLVAISLTLRLAQATVPADLSELRSRLGRVTDELTEATEEL
                     RELARGIHPAILSEGGLGPALRTLARRAAIPVELDIRTDTRPAEPIEVAAYYVVSEAL
                     TNTTKHARASHTDVTVEQHDTQLHLSIRDDGVGGADPAAGSGLIGLRDRVQALGGTIE
                     VSSRRGDG"
BASE COUNT          177 a          516 c          635 g          219 t
ORIGIN      
        1 ccgtcccccc ggcggctgct gacctcgatc gtcccgccca gggcctgtac ccggtcgcgc
       61 agcccgatca ggcccgaacc tgcggcggga tcggcgccgc cgacgccgtc gtcgcggatc
      121 gacaggtgca gctgggtgtc gtgctgttcg acggtgacgt ccgtgtggga ggcgcgggcg
      181 tgtttggtgg tgttggtgag cgcttcggac acgacgtagt aggcggccac ctcgatcggc
      241 tccgcggggc gggtgtcggt gcggatgtcg agttccaccg ggatcgcggc gcgtcgggcg
      301 agggtgcgca gggccggccc gagcccgccc tcggacagga ttgccgggtg gatgccgcgg
      361 gcgagctcgc ggagctcctc ggtcgcctcg gtgagctcgt cggtgacccg gccgagccgg
      421 ctccggagct cggacaggtc cgcgggcacg gttgcctggg ccaggcgcaa cgtgaggctg
      481 atcgcgacga ggcgttgctg ggtgccgtcg tgcaggtcgc gttcgatgcg gcggcgggcg
      541 tcgtcggcgg cggcgacgat ccgggcccgc gacgcagtga gctgggcgcg gctgtcggcg
      601 ttggcgatcg cggtgccgat gagttcggtg aacccggcca tgcggtgttc ggtgtcggcc
      661 gggaaacgtc cgcgccgcgt cccgacgccg atcgcgcccc acagccgtcc ctcgacggtg
      721 atcggggccg cgaccgagga ccggagtccc agccggcgga cggcgtcggc gtagtcgccg
      781 ggggcttggc tgtagttgtc gcagcgagcc gggcggcacg tgcgcaaggc gaccgccacg
      841 gccacgggcg gctcaggctt ccagcggctg cccaccggga gctcggcgag gtgtgcgccg
      901 acgctggcga cgagagtggt cacggggccg tgatcgaggc ggacgatgac ggtgccgtcg
      961 gcatcgagga cgctgccggc ctcgtgcgcg acggcggcga acacctcggc cggtggcagc
     1021 ccgcgggcga ccagcgtggc gacgcggcgc agcgcggcct gttcctcggc gagtcggcgc
     1081 agttcgtcgc ggctgtcctc gagttgggtg cggccttcgg cgttcgcgat cgcggtgccg
     1141 atgagttcgg tgaaaccggc caggcgctgt tcggtgtcgg ctgggaggtg ctcgcgccgc
     1201 gtcccggtgc cgatcgcgcc ccagagacgc ccctccacgg tgatcggagt cgcgaccgac
     1261 gaccggattc ccacccggcg gaccgtggcg tcgacgaacg cgccggaggc gtggctgtag
     1321 tcgtcgcagc gagccgggcg gccggtgcgc aaggcgaccg ccagggccag cggcggctca
     1381 ggggtccagc ggctgcccac cgccagatcg tcggagtgat cgccgacgac ggcgaggacg
     1441 gtcatcgcgc cgtcgggatc gaggcgaccg atggtcgtgg cgtcggcccc gaggacgtgg
     1501 ccgacctcgt gcgcgacggc ggcgaacacc tcggccggtg gtgtccc
//
LOCUS       DASYLX010000619         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1237060,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000619 DASYLX010000000
VERSION     DASYLX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237060"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VGP27_14685"
     CDS             <1..1830
                     /locus_tag="VGP27_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7852579.1"
                     /translation="GTVVLTVIFADQTLATVLEATRIRTEIGPSQAWYTENLRYYYLF
                     LPTVDGSLSRRFGFLITALSLFAAMFILLRRKRVPGVARGPAWRLIGIIFGTMFFLMF
                     TPTKWVHHFGLFAAVGAAMAALATVLVGPKVLRWSRNRMAVASAVLFVLALCFSSTNG
                     WWYVSSFGVPFNNAMPKIGGITVSTIFFALFALSAIYTVWLHFAPRDRGEGRIARALT
                     AAPIPVAAGFMVMVFVASMLVGVVRQYPTYSNGWANLRAFTGGCGLADDVLVEPDAND
                     GFMTPLPGSYGPLGPLGGDKPVGFTPNGVPDHIVAESIRVTLPMPGTDYDWDAAVKLT
                     DKGVNGSTVPLPYGLDPARVPVAGTYADGPQQQGKLTSAWYQLPKADDAHPLVVVTAA
                     GTIAGNSVLNGHTDGQPVELEYARPGPDGAPVPAGRLVPDDIAVAPSWRNLRFARSKI
                     PSDVVAVRVVAEDRSLTPGDWIAVTPPRVPELRSLQEYVGSEQPVLMDWAVGMAFPCQ
                     RPMLHANGVTEVPKFRITPDYTAKKQDTDTWQDGRNGGLLGISDVLLRAHVMATYLSR
                     DWGRDWGSLRKLDTIVDAQPADIQLGTATRSGLWKPNKIRVGA"
     gene            1956..2411
                     /locus_tag="VGP27_14690"
     CDS             1956..2411
                     /locus_tag="VGP27_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV7852580.1"
                     /translation="MRTAEDFQQMLRGAALRVTRPRVAVLTAVHAHPHADTDSIIRAV
                     RSDLPDVSHQAVYDSLSTLTAAGLVRKIQPSGSVARYESRVGDNHHHVVCRSCGVIAD
                     VECAVGEAPCLTASDDDGSLAGFAIDEAEVIYWGLCPDCAATNIFRSQP"
     gene            2448..>3730
                     /locus_tag="VGP27_14695"
     CDS             2448..>3730
                     /locus_tag="VGP27_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEV7852581.1"
                     /translation="MSSDTSDARPSTPAADTASHSESENPAIRSPEPKAHRPLNNQDW
                     WPNQIDVSVLHAHSNKGNPLGEDFDYAEEFAKLDPEALKADVMSVITTSQDWWPADYG
                     SYAGLFIRMSWHAAGTYRTYDGRGGGGQGMQRFAPLNSWPDNANLDKARRLLWPVKKK
                     YGNKISWADLLVFAGNVALESAGFETFGFGFGRPDFWEPEEIIFGEEEEWLGTDKRYA
                     GADASARQLEERYGATTMGLIYVNPEGPEGKPDPLAAAHDIRETFGRMAMNDEETAAL
                     IVGGHTLGKTHGAGDGDLVGPEPEAAPIEQQGLGWKCAFASGKAGDAITSGLEVVWTP
                     TPTQWSNAYLELLYGHEWELTKSPGGAWQFEAKDAEEIIPDPFDPNKKRKPTMLVTDV
                     SMRVDPIYGQITRRWLDHPEELNEAFAKAWYKLLHR"
BASE COUNT          630 a         1247 c         1243 g          610 t
ORIGIN      
        1 ggcacggtgg tgctgacggt gatattcgcc gaccagacgc tggcaacggt gttggaggcg
       61 accaggattc gcacggagat agggccgagc caggcttggt acaccgagaa cctgcgctac
      121 tactacctgt tcctgccgac cgtcgacggc tcactgtcga gacggttcgg cttcctcatc
      181 actgcactga gcctgttcgc ggccatgttc atcctgttgc ggcgcaagcg cgtccccggc
      241 gtcgcccgag gcccggcatg gcgcctgatc ggcatcatct tcggcacgat gttcttcctg
      301 atgttcaccc cgaccaagtg ggtgcatcac ttcggtctgt tcgccgccgt cggtgcggcg
      361 atggcggcgt tggccaccgt gctggtcggg ccgaaagtgc ttcgctggtc ccgcaaccgg
      421 atggcggtcg cgtcggcggt gctgttcgta cttgcgcttt gcttctcgtc gaccaacggc
      481 tggtggtacg tctcgagctt cggcgtgccg ttcaacaacg cgatgcccaa gatcggcgga
      541 atcacggtca gcacaatctt cttcgcgctt ttcgcactgt ccgcgatcta cacggtgtgg
      601 ctgcacttcg ccccgcgtga ccgcggtgag ggccgcatcg cgcgtgcgct gacggcggcg
      661 ccgattcccg tcgcggccgg cttcatggtc atggtgttcg tcgcctcgat gctcgtcggg
      721 gtcgtgcggc agtacccgac gtattccaac gggtgggcca acctgcgcgc gttcacgggc
      781 gggtgcgggc tggccgacga tgtgctcgtg gaaccggatg ccaacgacgg cttcatgacg
      841 ccgctgccgg gcagctacgg accgctgggt ccacttggcg gcgacaaacc cgtcggcttc
      901 acccccaacg gcgtgcccga tcacattgtg gccgaatcga ttcgggtcac cctgccgatg
      961 ccggggacgg actacgactg ggatgcggcc gtgaagctca ccgacaaggg cgtcaacggg
     1021 tcgacggtgc cgctgcccta cgggctcgac ccggcgcggg tcccggtggc cggcacctat
     1081 gccgacgggc cgcagcagca gggcaagttg acctcggcgt ggtatcagtt gccgaaggcc
     1141 gacgatgccc acccgctggt ggtggtcacc gcggcgggca cgatcgcggg caacagcgtg
     1201 ctcaatgggc acaccgacgg ccagccggtc gagttggagt acgcgcggcc cggccctgac
     1261 ggtgctccgg tgcccgcggg ccgcctggtg cccgacgaca tcgcggtcgc cccgtcgtgg
     1321 cggaatctgc ggttcgcgcg gtcgaagatt ccctccgacg tggtcgcggt tcgtgtcgtc
     1381 gccgaggacc ggtcgctgac gcccggcgac tggatcgccg tcaccccgcc gcgggtgccg
     1441 gagcttcgct cgctgcagga gtacgtcggg tccgagcagc cggtgctgat ggactgggcg
     1501 gtcggaatgg cgttcccgtg ccagcggccg atgctgcacg ccaacggggt gaccgaggtg
     1561 ccgaagttcc ggatcacgcc ggattacacg gccaagaagc aggacaccga cacctggcag
     1621 gacggccgca acggcgggct gctcggcatc tccgatgtgc tactgagggc ccatgtgatg
     1681 gcgacgtacc tgtcgcggga ctggggccgc gactgggggt cgctgcgcaa gctcgacacc
     1741 atcgtcgacg cccagcccgc cgacatccaa ctcggcacgg cgacccgcag cggactgtgg
     1801 aagcccaaca agatccgcgt cggcgcttga ccgcacggtg ctgacggcgg cttcggggca
     1861 tggcccgcgg cgaccgcttt cggacgacga tcggctcgga atagcgccat gtagatttct
     1921 tgacttattc cagaaaaggg acaagaattt cggacgtgcg cacggcggag gacttccagc
     1981 agatgctgcg gggagccgcc ctacgcgtca cccgtcccag ggtcgcggtg ctgaccgcag
     2041 tgcacgctca tccgcatgcc gacacggatt cgatcatccg ggcggtgcgt tcggacctgc
     2101 ccgatgtgtc ccatcaagcc gtctacgact cgttgagcac actgacggcg gccgggctgg
     2161 tgcgcaaaat ccagccgtcc ggttcggttg cccgctatga gtcgcgggtg ggcgacaatc
     2221 atcaccacgt tgtctgccgc tcatgcggtg tcatcgccga tgtggaatgc gccgtcggcg
     2281 aagcaccatg cctgaccgcg tccgacgacg acggctccct tgccggcttc gccatcgacg
     2341 aggccgaggt catctactgg ggcctgtgcc ccgactgtgc ggccacgaac attttccgat
     2401 cacaaccgtg atcacagccc cgataaccca ctcccggaag gaaagttgtg tcatctgata
     2461 catccgatgc ccgcccgtcc acgcctgcag ccgacactgc cagccacagt gagagcgaga
     2521 acccggcgat ccggtcgccg gagccgaagg ctcaccgccc gctgaacaat caggactggt
     2581 ggcccaacca gatcgacgtc tcggtgctgc acgcgcactc gaacaagggc aacccgctcg
     2641 gcgaggattt cgactacgcc gaggagttcg ccaagctcga ccccgaggcc ctcaaggccg
     2701 acgtcatgtc ggtgatcacc acctcgcagg actggtggcc cgccgactac ggcagctacg
     2761 ccggcctgtt catccggatg agctggcacg ccgcgggcac ctaccggacc tacgacggac
     2821 gcggcggcgg cggacagggc atgcagcgtt tcgcccccct caacagctgg cccgacaacg
     2881 ccaacctgga caaggcccgc agactgctgt ggccggtgaa gaagaagtac ggaaacaaga
     2941 tctcgtgggc cgacctgttg gtgttcgcgg gcaacgtggc gctggagtcg gcgggtttcg
     3001 agacgttcgg gttcggcttc ggtcgtcccg acttctggga gccggaagag atcatcttcg
     3061 gcgaggaaga ggagtggctg ggcaccgaca agcgctatgc cggcgccgac gccagtgcgc
     3121 gtcagctcga ggaacgctac ggcgccacca ccatggggct gatctacgtc aaccccgaag
     3181 gccccgaggg caagccggat ccgttggccg cggcccacga catccgcgag acgttcggtc
     3241 ggatggcgat gaacgacgag gagaccgccg cgctgatcgt cggcggccac accctcggca
     3301 agacccacgg cgccggtgac ggtgacctgg tcggcccgga gcccgaggcc gctcctatcg
     3361 agcagcaggg cctcggctgg aaatgcgcgt tcgcgtccgg caaggccggc gacgccatca
     3421 ccagcggtct cgaagtggtc tggacgccca caccgacgca gtggagcaac gcctacctcg
     3481 aactgctcta tggtcacgaa tgggaactga ccaagagccc aggtggcgcg tggcaattcg
     3541 aggccaagga cgccgaggag atcatccccg accccttcga ccccaacaaa aagcgcaagc
     3601 cgacgatgct ggtcaccgat gtgtcgatgc gtgtcgaccc catctacggc cagatcaccc
     3661 ggcgctggct cgaccatccc gaggagctca acgaggcgtt cgcgaaggcc tggtacaagc
     3721 tgctgcaccg
//
LOCUS       DASYLX010000620         5734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_799947,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000620 DASYLX010000000
VERSION     DASYLX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799947"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VGP27_14700"
     CDS             <1..672
                     /locus_tag="VGP27_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7852582.1"
                     /translation="LGDNGAGKSTLIKIIAGYHPQTDGELLVDGEPTRFTSPKDALGK
                     GIATVYQNLAVVPLMPVWRNFFLGQEIRQSRFPWSLDANAMRATTLSELSKMGIDLPD
                     VDVPIGSLSGGQRQCVAIARAVFFGARVLILDEPTAALGVKQSGVVLKYITAAKEAGF
                     GVVFITHNPHHAHMVGDHFVLLNRGQQKLDCSYDDITLEHLTQEMAGGDELEALSHEL
                     RSAKG"
     gene            complement(1065..2027)
                     /locus_tag="VGP27_14705"
     CDS             complement(1065..2027)
                     /locus_tag="VGP27_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7852583.1"
                     /translation="MSLRIGVLGASRIADLAIVGPAQQLGHRLVAVAARDRGRAELFA
                     EKHGVERVLNSYADVIGDPEVNAIYNPLANSLHAPWNLAAVKAGKPVLTEKPFARNQS
                     EARHVAEAAKVADVAVVEGFHYLYHPVTRRALELAVDGTIGRMHHVEARMAMPEPDAS
                     DPRWSFDMAGGALMDLGCYGLHIMRRLGHPSIIRAHAKERAAGVDEWCDVELGFPGGA
                     TGLSANSMSAPDHSFTLRIVGTKGDLFVHNFIRPSEDDRLTIRTQSGTRVEHHGTRTS
                     YSYQLEAFAAHVLHGAPVPIDVADAVENMAYVDAAYRAAGMTPR"
     gene            complement(2024..2896)
                     /locus_tag="VGP27_14710"
     CDS             complement(2024..2896)
                     /locus_tag="VGP27_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7852584.1"
                     /translation="MKIAGAPISWGVCEVPGWGFQLAPDRVLAEMRDVGMSATELGPE
                     GFLPSDPAQLTAVLDSFGLRCVGQFAPVLLHDEDHDPLPDIAAPLDALSACDADVLVL
                     AAATGSDGYDARPALDDQQWATLLANLDRLGAAAADRGVLAVLHPHVGTMVETRDEVD
                     RVLGGSAIQLCLDTGHLLIGGTDPLQLARKVPDRIAHAHLKDVDAALAARVRAGELSY
                     TEAVRRGMYTPLGAGDVDIAGIVTALRRNGFDGWFVMEQDTILDGEPKDDGPLSDVAA
                     SVTFMQDVCRSVTV"
     gene            complement(2893..3900)
                     /locus_tag="VGP27_14715"
     CDS             complement(2893..3900)
                     /locus_tag="VGP27_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7852585.1"
                     /translation="MTTLGVIGLGRIGAFHTDTLSGLKGVDGLVITDERPQLVAAVAA
                     KHSVTPADSVDKLLSSGIDGVVIAAATPAHAELTLAAVERGIPTFCEKPIATTAEESV
                     KVAEIIARSGVPVQVGYQRRFDAAFAAAKSAVDDGSLGLLHTVRSTTMDPAPPPMDYI
                     AGSGGIFRDCAVHDFDILRWITGQEAVEVYATGSVQGDPQFADFHDVDTAAVVVRFDS
                     GTLGVISAARYNGHGYDCRLEVHGFHDTVVAGWDQGAPVRNVDPGNEFPEGPAHHFFM
                     DRFTEAFRAELAAFVDVANGAAAPACTVEDAVEVAWLAEAATESLKRGVPVRLEDVRS
                     R"
     gene            complement(3897..5054)
                     /locus_tag="VGP27_14720"
     CDS             complement(3897..5054)
                     /locus_tag="VGP27_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV7852586.1"
                     /translation="MATPLTTAYKAWIDESDCGLDSFRAEVAQNTDLADYPHAVDVRQ
                     NVLVYPSTTPSDRAALQAELIHALIDGPGVVVFEDAFSSGAIERASEAFYAIIEAQRA
                     AGTAAGDHFGKPGDNDRIWNAAQKLAVHAPEVFAEYYANDTLAVVSQAWLGPRYQVTS
                     QVNVVNPGGKQQVPHRDYHLGFVSTDDLGAYPAHLHRASPVLTLQGAVAHCDMPIESG
                     PTMLLPYSQQFVGGYIAFNRPEFVAFFADNHVQVPLHTGDAVFFNPALYHGAGSNVST
                     DIRRMANLLQVSSPFGRAMEAMDRTAMVRATYPALLAMKAAGRPQRELHNAVVATAEG
                     YPFPTNLDSDQPIGSLAPPSQVDTVLAALAGDLTPEQLDVALRDQDDRRKP"
     gene            5132..>5734
                     /locus_tag="VGP27_14725"
     CDS             5132..>5734
                     /locus_tag="VGP27_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7852587.1"
                     /translation="MHRYKVREIAQQSGLSEATVDRVLNKRPGVRENTRAEVVQAIAD
                     LDKQRAQLRLNGRRYLIDVVMQTPQRFSDAFRAAVEAELPAFAPAMLRARFHLWETGS
                     TAQMVDALARLRGSHGVILKAQDDPGVAEAIDGLVEGGVPVVTYATDVPASARCSYVG
                     IDNHGAGVTAAYLMGQWLGSAPSDVLITVSRNVFRGEGERE"
BASE COUNT          991 a         1872 c         1917 g          954 t
ORIGIN      
        1 ctgggcgaca acggcgcagg caagtccacc ctgatcaaga tcatcgcggg ctaccatccg
       61 cagaccgacg gcgaactgct ggtcgacggc gagccgacca ggttcacatc gccgaaggac
      121 gcgctgggca agggcattgc caccgtgtac cagaacctgg ccgtcgtgcc gctgatgccg
      181 gtatggcgca acttcttcct cggccaggag atccggcaga gccggttccc gtggtcgttg
      241 gatgccaatg ccatgcgggc gacgaccctt tcggagctgt cgaagatggg catcgatctg
      301 cccgacgtcg acgtgccgat cggatcgctt tccggcggcc agcggcagtg tgtcgcgatc
      361 gcccgcgctg tgttcttcgg tgcgcgggtg ctgattctcg acgagcccac cgcggcgctc
      421 ggcgtcaagc agtcgggcgt ggtgctgaag tacatcaccg cggcgaagga ggccgggttc
      481 ggcgtcgtgt tcatcacgca caacccgcat cacgcgcaca tggtcggcga ccacttcgtg
      541 ctgctgaacc gtggccagca gaagctggac tgcagctacg acgacatcac cctggagcac
      601 ctgacgcagg agatggcagg cggcgacgag ctcgaggcgt tgtcacacga actgcgaagc
      661 gcaaaggggt agcgccaaac acagaaccgc cctagcacgt gacctgacgt gcacggggcg
      721 attctgcctc tgcggctcgg ttcagctctt cttgaggaaa ttgtcgccgt tgtcgatccg
      781 cgagtaaacc cagccttggc ctgcgggaag atcaccgtgc gggccaccgg gagatccggg
      841 acagcgagca ggcaccagtt gtccgcgcac ggctggacag ttgtgagcgt gcgaccaacg
      901 tcgagaaaaa atcccggaat ctcatcaccg ccgattgtcg ggtaaacact cgactgcttg
      961 agtacagtca ccgtcggctc cgcaggagcg gtgtcggccg gtttcggatc cgccggcatg
     1021 tttccgtctc gctggctcca cgacggctgg gcgctgcgga cgaactaacg cggtgtcatg
     1081 cctgcggctc ggtatgcggc atccacatac gccatgttct ccaccgcatc ggcgacgtcg
     1141 atcggcacgg gcgcgccgtg gagcacgtgc gcggcgaacg cctcgagctg atagctgtat
     1201 gaggtcctgg tgccgtggtg ctcgactcgt gtgcctgact gtgtccggat ggtcagccgg
     1261 tcgtcctcgc tcggcctgat gaagttgtgc acgaagagat ctcccttcgt gccgacgatg
     1321 cgaagcgtga acgagtggtc aggggccgac atcgagttgg cgctgaggcc ggtcgctccg
     1381 ccggggaagc cgagttccac atcgcaccat tcgtcaacgc cggcagcgcg ttccttcgcg
     1441 tgcgcacgaa taatcgacgg atggccgagc ctgcgcatga tgtgcagccc gtaacagccg
     1501 agatccatca gcgccccgcc tgccatgtcg aatgaccaac gcgggtcgga agcgtcgggc
     1561 tctggcatcg ccatccgcgc ctcgacatgg tgcatccggc cgatcgtgcc gtcgacggca
     1621 agctcgagcg ctcggcgcgt caccggatgg tagaggtagt ggaagccctc gaccaccgcg
     1681 acatcggcaa ccttcgcggc ctcggcgacg tgccgggcct ctgactggtt gcgcgcgaac
     1741 ggtttctcgg tgagcaccgg cttgccggcc ttgaccgcag cgagattcca cggcgcgtgc
     1801 agtgaattcg ccaaggggtt gtagatcgcg ttgacttccg ggtcgccgat gacgtcggcg
     1861 taggaattca aaacccgctc gacaccgtgc ttttcggcga acagctcagc gcggccccga
     1921 tcgcgcgcgg cgaccgctac cagccgatga cccaactgct gtgcaggacc gactatcgcg
     1981 aggtccgcga ttcgggatgc gccgagcacc ccgattcgca aactcacacc gttacgctcc
     2041 ggcatacatc ctgcatgaag gtcacactag ccgccacatc agatagcgga ccgtcgtctt
     2101 tcggctcacc gtcgagaatg gtgtcctgct ccatgacgaa ccagccgtcg aatccgttgc
     2161 gccgcagcgc cgttacgatg cccgcgatgt ccacgtcgcc cgctccgagc ggcgtgtaca
     2221 taccgcgtct caccgcttcg gtgtaggaca gctcgccggc ccgcactcgc gcagccaaag
     2281 ccgcgtcgac gtccttcagg tgggcatgcg cgatgcggtc aggtaccttc cgggccagct
     2341 gcagcggatc cgtcccgccg atcagcagat ggccagtgtc gaggcagagt tggatcgccg
     2401 agcccccgag tacccgatcg acctcatcgc gcgtctccac catcgtgccg acgtgcggat
     2461 gcagcaccgc cagcacgccg cggtcggcgg cggccgcgcc gagccggtcg aggttggcca
     2521 gcaatgtcgc ccactgttga tcatcgagtg cgggtcgggc gtcgtatccg tcggatccgg
     2581 tcgcggcggc caacacgagc acatcggcgt cgcacgccga cagcgcgtcc agcggtgcgg
     2641 cgatgtcggg cagcgggtca tgatcctcgt cgtgcagcag gacgggggcg aactggccga
     2701 cacaacggag accgaacgag tcgagcacag cggtcagctg cgctggatca gagggcagga
     2761 acccttccgg cccaagctcc gtcgccgaca tcccgacgtc gcgcatctcg gcgagcactc
     2821 tgtcaggtgc gagctgaaat ccccatccgg gcacttcgca cacaccccag gagatgggtg
     2881 caccagcgat cttcatcgag acctcacatc ctccaggcgt accggaacac cgcgcttcaa
     2941 cgactcggtc gccgcctcgg ccagccaggc cacctcgacc gcgtcttcga cggtgcaggc
     3001 gggagcggca gcgccgttcg ccacgtcgac gaaggctgca agctccgccc ggaacgcctc
     3061 ggtgaagcgg tccatgaaga aatggtgcgc agggccctcc gggaactcgt tgccgggatc
     3121 gacatttcgc accggtgcac cctggtccca tccggcgacc accgtgtcgt ggaagccgtg
     3181 cacctcgagg cggcagtcgt agccgtggcc gttgtagcgg gccgccgaga tcacgccgag
     3241 ggtgcccgaa tcgaatcgca ccaccacggc cgcggtgtcg acgtcatgga agtcggcgaa
     3301 ctgcgggtcg ccctgcacgc tgccggtggc atagacctcg acggcttctt gtccggtgat
     3361 ccaccgcagg atgtcgaaat cgtggacggc acaatcccgg aagatgcctc cggagcctgc
     3421 gatgtagtcc atcggtggcg gcgccgggtc cattgtcgta ctgcgcaccg tgtgcagaag
     3481 gccgagcgag ccgtcgtcga cggcactctt cgcggcggcg aacgcggcgt cgaagcgacg
     3541 ctggtaaccg acctgcaccg gtacgccgga gcgcgcgatg atctcggcga ccttgacgct
     3601 ctcctctgca gtcgtggcga tcggcttctc gcagaacgtg ggaatcccgc gctcgacggc
     3661 ggccagcgtc agctcggcat gggcgggcgt cgccgcggcg atcacgacgc cgtcgatacc
     3721 cgacgagagc agtttgtcga ccgagtcggc cggagtcaca gagtgtttcg cggcgacagc
     3781 ggcgacgagt tgcggccgct cgtcggtgat cacgagaccg tcaacgccct tcaggccgct
     3841 gagggtatcg gtgtggaatg cgccgatacg gcccaatccg atcacgccga gtgtggtcat
     3901 ggttttctcc gatcgtcttg gtcgcgcagt gcgacatcga gttgttccgg ggtgaggtca
     3961 ccggccagcg cggccagcac ggtgtcgacc tggctcggcg gggcgaggct gccgatcggc
     4021 tgatcggagt cgaggttggt gggaaaggga tagccctcgg ccgtcgcgac gacggcgttg
     4081 tgcagttcgc gctgcggacg gcctgcggcc ttcatcgcca gcagcgccgg ataggtcgcc
     4141 cgcaccatcg cggtgcggtc catcgcttcc atcgcccggc cgaagggcga cgagacctgc
     4201 agcaggttcg ccatccgtcg gatatcggtg gacacgttgg agccggcacc gtgatacagc
     4261 gcgggattga agaagaccgc atctccggtg tgcagcggca cctgcacgtg gttgtcggcg
     4321 aagaatgcga cgaactccgg ccggttgaac gcgatgtatc cgccgacgaa ctgctgggag
     4381 tagggcagca acatcgtggg cccactctcg atcggcatgt cgcagtgcgc caccgcgccc
     4441 tgcagggtca gcaccggcga cgcgcggtgc agatgcgccg ggtacgcgcc cagatcgtcg
     4501 gtcgagacga agcccaggtg atagtcacgg tgcggaacct gctgctttcc accgggattg
     4561 accacattga cctgcgaggt gacctggtag cgaggaccta gccacgcctg cgagacgacg
     4621 gcgagcgtgt cgttcgcgta gtactccgcg aacacctcgg gggcatgcac ggcgagcttc
     4681 tgtgcggcat tccagatgcg gtcgttgtcc cccggcttgc cgaagtgatc gcctgcagct
     4741 gttcccgccg cccgctgcgc ctcgatgatg gcgtagaacg cctcgctggc ccgctcgatg
     4801 gcgccggagg agaacgcgtc ctcgaacacg acgacgccag ggccgtcgat cagcgcgtgg
     4861 ataagttcgg cctgcagggc tgcccggtca gacggcgtgg tggaggggta gacgagaacg
     4921 ttctgccgga cgtcgacagc gtgcggatag tcggcgaggt cagtgttctg cgctacttcc
     4981 gcccgaaacg agtcaagccc gcagtcggac tcgtcgatcc acgccttgta agcggtggtc
     5041 agaggtgttg ccatggcagc tagtctggta gtcggatgca tcacaatcaa caccaaaaac
     5101 ccatcaaaaa aacctcagaa ggacggtccc cgtgcaccgg tacaaggtcc gcgagatcgc
     5161 ccagcaatcc gggttgagcg aggccaccgt cgaccgcgtg ttgaacaagc ggccgggtgt
     5221 gcgggagaac acccgcgccg aggtcgtaca ggcgattgcg gacctggaca aacagcgcgc
     5281 ccagctgcgg ttgaacggca ggcgttacct catcgacgtg gtcatgcaga ccccgcagcg
     5341 gttctccgac gcatttcgcg cggccgtcga ggccgagctg cccgccttcg cgcccgccat
     5401 gctgcgcgcc cgcttccatc tctgggagac cggctcgacc gcacagatgg tcgacgcgct
     5461 cgcccgcctg cgcggcagcc acggcgtgat cctcaaggcg caggatgacc ccggggtcgc
     5521 cgaggcgatc gacgggctgg ttgaaggcgg cgtccctgtg gtgacctatg cgaccgatgt
     5581 gcccgccagc gcgcgctgtt catatgtggg catcgacaac cacggcgcgg gcgtgaccgc
     5641 cgcctatctg atggggcagt ggctgggttc agcgccgtcg gacgtgttga tcaccgtcag
     5701 ccgcaacgtg tttcgcggcg agggtgagcg cgag
//
LOCUS       DASYLX010000621         6284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1128264,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000621 DASYLX010000000
VERSION     DASYLX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128264"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VGP27_14730"
     CDS             complement(<1..1404)
                     /locus_tag="VGP27_14730"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852588.1"
                     /translation="MNGKLVDGRDPDRKLDRRIDSLVTNNLGTDLPIELPDGQWRYAI
                     ALQAVEGSKGALVIRAEVALSDYELFLLRALAQQTAAAMTSADLLDKERAFIEERQRA
                     IHRLSDTVSELKRQGQIHATLTAVSGSGSGVQGIADALRELTSLNVTVEDVFGNPRAW
                     SGGEEPEPHRPIGGDNREEILRRAAAHGTPQRDGNRIFWLIRQKANILGVVLLHDPNS
                     HADRLDIVALEHAATVLALEFAHQRVLAETELRLRRDLVEDLLAGTDDESAFLRAEAL
                     GHNLRVPHTVTVLHWRQGVPGEMIATSARRWATNARMHSLAVRRPTVTVLVTDGMPEP
                     SSLYRAIAADVASEQGAIGIGSAAATPSELPRSLAEAQRALQVQRASMSPYGGRRFED
                     LGLYRILDRTGDRPEVHDFVMEWLGPLLRYDREKKADLVMTLTQYLDCGGNYDATAQS
                     LTIHRSTLRYRLGRIRDI"
     gene            2317..3003
                     /locus_tag="VGP27_14735"
     CDS             2317..3003
                     /locus_tag="VGP27_14735"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEV7852589.1"
                     /translation="MSDALELGVTGIEAALGDHICGLYSNELQRDQILLPFLEAGLAA
                     GDKCICVVDGTDPKEVIAALDTRVDAAKFAANKQLDVIRAPDMYMRSGGFSADEVISS
                     WKAAISDVMYDGRFDVVRAVETWSRRDVVPDMDELMMLESEMNHFLPLFPQVVVCLYD
                     IERFGSGIIVNLLKTHPRMLIGGMLVENPYYTSPDELLHRAERGAPETADAEINEAAK
                     WYFDETTGSN"
     gene            2970..4451
                     /locus_tag="VGP27_14740"
     CDS             2970..4451
                     /locus_tag="VGP27_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene monooxygenase"
                     /protein_id="HEV7852590.1"
                     /translation="MVLRRDDWLELARKVNWTPRYVDEREMFPEVLSGHPWLAHDAWR
                     DWNEAYRTTYREYVANQREKDAAVLGVRSALDKPHLLDGLDPGWVQLVKFHNGAVALV
                     EYAGAIAELRMARFGRDSAWRMMSSLGALDEIRHTQIPLLIGHDMLAYDGNFDWTHKA
                     FHTNQWVILAARHLFDDMFLAADAIDSAIQLNFVFETGFTNLQFMAMAAMADRAHHHL
                     FEKTLASIQTDEARHAQIGHPVMRTLLENGSRERAQYLVDKMWWRCWRLFLALTGTAM
                     EYLTPLDARTRSFKEFMKEWVAEQFMKNLAEFDLEKPWFWDLFVEELDYAHHSFQLGL
                     YTYRTTLWFDVAMPDDAEREWLNTKYPDWEATYGPLWDRLERSWKAGGESNTLSYGLP
                     ALCNLCQLPALFVRPGRNTACTLEMDGRRYLFCSEPCRWIFQQESARFADHKSVVDRI
                     VVGEAPGKLTDLHKWMGLEAPVETGRDLRRGLDPWRLDPIPTA"
     gene            4462..4698
                     /locus_tag="VGP27_14745"
     CDS             4462..4698
                     /locus_tag="VGP27_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852591.1"
                     /translation="MRIYLFGYASDDFLARVVVTPNERTVAQLADQLVAWGLEPERGG
                     SRSVRNEIGDLLDPNSTIAAAGLGNGDIFTVERG"
     gene            4667..5023
                     /locus_tag="VGP27_14750"
     CDS             4667..5023
                     /locus_tag="VGP27_14750"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7852592.1"
                     /translation="MVTSSPSSGGDGALGDWLDAGTLEDVWEGEMCAVSLGAVDVLLC
                     NIDGQVHAYQDRCPHLANPLSEGELRDNILTCAAHEWVFDARTGNGVNPEDACLRRFP
                     VRVDGDRILVLLGQVG"
     gene            5020..5310
                     /locus_tag="VGP27_14755"
     CDS             5020..5310
                     /locus_tag="VGP27_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HEV7852593.1"
                     /translation="MSGAESVGPVLHSTPFARTVISAIRDENEQVIVQDEGAYLRVLV
                     PRMCRLSRAGLEAATGVTVRFPGELEVVMSSFTGVMQLTEDDVVWQLAGEPS"
     gene            5307..>6284
                     /locus_tag="VGP27_14760"
     CDS             5307..>6284
                     /locus_tag="VGP27_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene hydroxylase"
                     /protein_id="HEV7852594.1"
                     /translation="MTPPDRPPRRKTYTRLATGRRIPTEYELMSTDLHYNHPRRFELP
                     AGNPVVDWYYRNREGSALHERDWEQFADPRRTTYQIYTGLQDAREDVVDGLLREVDDT
                     AYDTQLDDEWVRFLDRWYSPLRFPVHGLQMLAAYVAQMAPSSRITNCASFQTGDEMRR
                     VQRIAYRTVQLAGPPFDDESATRQRSAWEDAAAFQPLRELIERALVAYDWGESFIVTN
                     AVIKPRIDRLVNQDIAGTLATANGDPILTSIHFSLDEDARWHREWTAALLRHIVDDNT
                     ANAEVVSGWIEKWSPLATEALETFAGVTAEAPVPSDPSEVAARITDEVSR"
BASE COUNT         1160 a         1980 c         2000 g         1144 t
ORIGIN      
        1 gatgtcgcga atacgtccaa gccgatagcg caaagtgctt ctgtggatag tgagcgactg
       61 cgccgtcgcg tcgtagttgc cgccgcaatc cagatactgc gtgagtgtca tgaccaaatc
      121 ggccttcttc tcgcggtcat acctcagcag tggacccaac cattccatta cgaagtcgtg
      181 aacctctggc ctgtctccag tgcgatcaag gatgcggtac aggccgaggt cctcgaagcg
      241 ccggccaccg tacggtgaca tcgatgccct ttggacctgc agcgcccgtt gcgcctcagc
      301 gagcgacctt ggcaactcgg acggagtcgc ggccgccgag ccgatgccga tcgcgccttg
      361 ttcggaagcg acatcggctg ctatcgcgcg gtaaagagag gacggctcgg gcataccgtc
      421 ggtgaccaag actgtcactg ttggtctacg taccgcgagg gaatgcatcc gcgcgttagt
      481 ggcccaccgt cgcgcggatg tggcgatcat ttcaccgggg actccttgcc gccaatgcag
      541 aaccgtgacg gtgtgtggga cgcgaagatt gtgaccgagt gcctccgcgc gcaggaatgc
      601 gctctcgtcg tccgtgccgg ccagcaagtc ctccactagg tcgcggcgca gccgcagctc
      661 cgtttcggct aggacgcgtt gatgggcaaa ctcaagtgcc aacacagtcg cagcatgctc
      721 tagcgcgacg atgtccagac gatcggcgtg gctgttcgga tcgtgcagga gcaccactcc
      781 caggatgttc gccttctgcc ggataagcca gaagatccga ttgccgtcac gctggggtgt
      841 gccatgcgcg gccgcgcgcc ggagtatttc ttcgcggttg tcaccgccga ttggccggtg
      901 cggttctggc tcctctccgc cagaccacgc ccgcggattg ccgaacacgt cctcgacagt
      961 gacgttcaaa gacgtaagct cccgaagcgc atcagcgatt ccctgcacgc ccgagcccga
     1021 gccggatact gccgtcaggg tcgcgtggat ctggccctgc cgcttgagct cggaaacggt
     1081 gtcggacaac cggtgtatgg cacgctgacg ttcctcaatg aatgcgcgct ccttatccag
     1141 caggtcggca ctcgtcatcg ccgctgccgt ttgttgggcc aatgcccgga gcaggaacag
     1201 ctcgtagtcg gagagggcga cttcggcccg gatgactagc gcacccttgc taccttccac
     1261 cgcctgtagg gcgatcgcat atcgccactg accgtcgggc agctcgatgg gaagatccgt
     1321 ccctaagttg tttgtgacca gggagtcaat gcggcgatca agcttcctat cgggatcacg
     1381 accgtctacc agcttcccgt tcatgattcg gtacgtcgcg tcggtgcgaa acgcgccgag
     1441 agacgacacc gcgttggccg ctaactcgag tatcgaatcg gcgtcgcgac cgtcgaacat
     1501 caacgccgac aggacaagga cgccatacag gttcgacagc tgagcgcggg cgtcctccga
     1561 tgagcgcagc gcaatccgcc tggaggagaa gaagtcaagg ggctcacgcg ccgcagtcaa
     1621 ttatcccgcc ccccaagccc gtatcaacca gtgattgcct ccacatcgtc acacaaccag
     1681 aacgcgccct cttcgtcctc gcaggacagt ttcgggcccg acgtattgca accacagcat
     1741 gattggcaaa cattgtgacg gcacgttcga cagccgggaa acggcagcta gtgcgcgcaa
     1801 tcaacacgtg aagcgccgtg gctagttcag tcgtggacgc cgacgttgaa ccagctgcct
     1861 tctgcccaat gtgataccgc aggtctaagt tgccgtcgcg gggtgactgc gacgtcccag
     1921 cgcgccggtc atagtcctgc tgtgaccagc gacttggttt ctcccaacga ccgactcgaa
     1981 ggggctattg cgcatgaccg tttacttcgg aatcgctggg ctccacatga gccgcaacgc
     2041 tctcattgtc ttcactgcga tggtcggaga tgttttgtct tggcgcgcag tctcttccgg
     2101 ggcggtgagg gtgcgccgtt ggtggcgcag cgtgacgagt cgtgttgcgc ggtcgatgag
     2161 ccagggtgag ctgcgggagc gccaaccgtc aggccttcgc gccgatcatc ccgccaaacg
     2221 gccgccaaag ttcgtcacgc ggtgacggtt ctttattccg caggagtctc taaagtggtc
     2281 tcttcgagca acttcagccg atcggggggt ggcgcagtgt ccgatgccct cgaattgggc
     2341 gtcacgggca tcgaagcggc gctgggcgat cacatctgtg gcctgtactc caacgaactc
     2401 cagcgcgacc agatcctgct gcccttcctc gaggcaggtc ttgcggccgg cgacaagtgc
     2461 atctgcgtcg tcgatgggac cgatccgaaa gaggtgatcg cggccctcga cacccgcgtc
     2521 gacgccgcga agttcgcggc gaacaagcag ctcgacgtca tccgtgcacc cgacatgtac
     2581 atgcgatccg gcggcttttc tgccgatgag gtgatcagct cctggaaggc cgcgatatcc
     2641 gacgtcatgt atgacggccg gttcgacgtg gtgcgggctg tggagacgtg gtcacgccgt
     2701 gacgtcgttc ccgacatgga cgaactcatg atgctcgagt ccgagatgaa tcacttcctg
     2761 ccactgttcc cgcaggtcgt cgtgtgcctt tacgacatcg aacgcttcgg cagcggcatc
     2821 atcgtcaacc tactgaagac gcatccacgg atgctgattg gcgggatgct cgtcgagaac
     2881 ccctattaca cgtcacccga cgaactcctt caccgggcag agcgcggagc tccagagacc
     2941 gcggatgccg agatcaatga ggcggccaaa tggtacttcg acgagacgac tggctcgaac
     3001 tagcccgcaa ggtcaactgg acgccccgct atgtcgacga gcgggagatg tttcctgaag
     3061 tactcagcgg ccacccctgg ttggcacatg atgcttggcg cgactggaac gaggcttacc
     3121 gcaccaccta ccgcgaatac gtcgcaaacc agcgcgaaaa agacgccgca gtgctcggcg
     3181 tgcggtcggc gttggacaaa ccgcatctgt tggacggcct cgatcccggt tgggtgcagc
     3241 tggtcaagtt ccacaacggt gcggttgcgc tcgtggaata cgccggcgcg atcgcggagt
     3301 tgcggatggc gcgcttcggc cgagacagcg cctggcggat gatgtcgagc ctgggcgcgc
     3361 tcgacgagat ccggcacacc cagatcccgt tgctgatcgg ccacgacatg ctggcttacg
     3421 acggcaactt cgactggacg cacaaggcct tccataccaa ccagtgggtg attctcgccg
     3481 ctcgtcacct gttcgacgac atgtttctcg ccgccgatgc aatcgacagc gcgatccagc
     3541 tcaacttcgt gttcgagacc ggcttcacca acctgcaatt catggcgatg gcggcgatgg
     3601 ccgaccgcgc ccaccatcac ctgttcgaga agacgctggc gagcattcag accgatgagg
     3661 cccgccacgc ccagatcggc catcccgtca tgcggacgtt gctcgagaac ggttctcggg
     3721 agcgggcaca gtacctcgtc gacaagatgt ggtggcggtg ctggcggctg ttcctggcgc
     3781 tgacgggaac agccatggag tacctcaccc cgctcgacgc gcggacccgc tcgttcaagg
     3841 agttcatgaa agagtgggtg gcggaacagt tcatgaagaa tctggccgag ttcgacctcg
     3901 aaaagccttg gttctgggac cttttcgtcg aagagctcga ctacgcacac catagcttcc
     3961 agctggggtt gtatacctac cgcaccaccc tgtggttcga cgtggccatg cctgacgatg
     4021 ccgagcgcga gtggttgaac acaaagtatc cggactggga ggcgacctac ggaccgctgt
     4081 gggatcggct cgagcggagc tggaaggcgg gcggcgagag caacacgctg tcctatggcc
     4141 tgcccgctct gtgcaacctc tgtcagcttc cggcgctgtt cgtcaggccc ggacggaaca
     4201 ccgcatgcac cctggagatg gacggccgac gctatttgtt ctgctccgaa ccgtgccgct
     4261 ggatctttca gcaagaaagt gcccggttcg cagaccacaa gagcgtggtc gatcgcatcg
     4321 tcgtcgggga ggcgccgggc aagctcacgg atttacacaa gtggatggga ctcgaggcgc
     4381 ccgtcgagac cggcagggac ctgcggcgcg gactcgatcc gtggcgcctc gatcccatcc
     4441 cgacggcgta ggggtgcggc ggtgcggatc tatcttttcg gttacgcgag cgacgacttc
     4501 ctggcgaggg tcgtggtgac acccaacgaa cgcaccgtcg cccaactggc tgatcagctg
     4561 gtcgcctggg gcctggagcc cgagcggggc gggtcgcgca gcgtgcgcaa cgagataggc
     4621 gacctgctcg atcccaattc gacgatcgct gcggcaggac tgggcaatgg tgacatcttc
     4681 accgtcgagc gggggtgacg gcgcattggg ggactggctg gacgccggga cgctcgaaga
     4741 tgtctgggag ggcgagatgt gcgcagtgag cctgggtgcc gtggacgtgc tgctgtgcaa
     4801 cattgacggc caggtgcacg cctatcagga ccggtgccct catctggcca acccgttgtc
     4861 ggagggcgaa ctccgggaca acatcctcac ctgtgctgcc cacgagtggg tgtttgacgc
     4921 acgaaccggc aacggagtca accccgagga cgcttgccta cgccgctttc cggtgcgagt
     4981 cgacggcgac cgcatcttgg tgcttctggg gcaggtcgga tgagcggagc cgaaagcgtt
     5041 ggcccggtcc tgcactcaac gccgttcgca cgcacggtca tctcggctat ccgggacgag
     5101 aacgaacagg taatcgtcca ggacgaaggc gcctacctac gcgtgctggt ccctcgaatg
     5161 tgccggctgt cgcgggcagg gctcgaggca gcgacggggg tgaccgtccg attccccggt
     5221 gagttggagg tggtgatgtc gtcattcacg ggcgtcatgc agctgacgga ggatgacgtg
     5281 gtctggcagc tcgcaggtga gccatcgtga cgccgccgga tcggccgccg aggcgcaaga
     5341 cctacacccg actggcgacg ggccggcgca ttccgaccga gtacgagctg atgagcaccg
     5401 accttcacta caatcacccc cggcgcttcg aactgccggc cggcaacccg gtagtcgact
     5461 ggtattaccg caatcgcgag ggctccgccc tgcacgagcg cgactgggag cagttcgccg
     5521 acccgcgccg caccacctac cagatctaca ccgggctgca ggacgctcgc gaagacgtcg
     5581 tcgacggcct gctccgggag gtcgacgaca cggcgtacga cacccagcta gacgacgagt
     5641 gggtgaggtt cctcgaccgc tggtattccc ctctgcgctt tcccgtgcac gggctgcaga
     5701 tgctggccgc ctacgtcgcg cagatggcgc cctcctcaag gatcaccaac tgcgcctcct
     5761 tccagacagg cgacgagatg cgtcgggtcc agcggatcgc ctaccgcact gtgcaattgg
     5821 ccggtccgcc gttcgatgac gagtccgcga cgcgacagcg gtcggcgtgg gaggacgctg
     5881 ccgcgttcca gccgctgcgt gaactcatcg aacgtgcact ggtcgcctat gactggggcg
     5941 aatccttcat cgtgaccaac gcagtcatca agccccggat cgaccgcctc gtcaaccagg
     6001 acattgcggg cacgctcgcg accgcgaacg gcgacccgat cctgacgagc atccacttct
     6061 cgctcgacga ggatgctcgc tggcatcgtg agtggactgc ggcgctgttg cggcacatcg
     6121 tcgacgacaa caccgccaat gcggaggtgg tgtcgggctg gatcgagaag tggagtccgc
     6181 tggcgaccga ggcgctggaa accttcgccg gcgtcaccgc ggaggcgccg gtgccgtccg
     6241 accccagcga ggtggccgcc cgcatcaccg acgaggtgtc gcgt
//
LOCUS       DASYLX010000622         5088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_909456,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000622 DASYLX010000000
VERSION     DASYLX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909456"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGP27_14765"
     CDS             <1..582
                     /locus_tag="VGP27_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7852595.1"
                     /translation="AALDKAREVRPDAVILDVMMPGMDGFGVLRRLRADGIDSPALFL
                     TARDTLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRSGRGTEEPRSAKLTFADI
                     ELDEDTHEVWKAGEPVSLSPTEFTLLRYFIINAGTVLSKPKILDHVWRYDFGGDVNVV
                     ESYVSYLRRKIDTGEKRLLHTLRGVGYVLREPR"
     gene            637..2103
                     /locus_tag="VGP27_14770"
     CDS             637..2103
                     /locus_tag="VGP27_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7852596.1"
                     /translation="MAGLRGRGVPLRVGLVAATLVLVACGLLASGIAVTSILRHSLTN
                     RIDETLLDASRSWAQAPRRMPVPPMEPNPARPPSDFYVRGVDPDGRIWMAVNDRAAEP
                     ALPANNDVGPAPVTVGSIDNSHVQWRAMSVHGPQDQITTVAIDLSDVQSTVRALIWSQ
                     AGIGAAVLLVLGVAGFWVVHRSLRPLTEVEQTAADIAAGQLDRRIPQRDPRTEVGRLS
                     LALNGMLAQIQRAVASSESSAEQARRSEERMRRFITDASHELRTPLTTIRGFAELYRQ
                     GAARDIEMLMSRIESESRRMGLLVEDLLLLARLDAQRPLDRHRVDLLALATDSVHDAR
                     SIAAKRKITVEVIDGPGTPEVLGDEARLRQVLGNLVANALQHTPETARITVRVGTDDD
                     NALLEVADEGPGMTPEDAHRVFERFYRTDESRDRASGGTGLGLSIVDSLVYAHGGSVS
                     VTTAPGRGSRFTVKLPRIADAVTHDVAASVRSGRPAPP"
     gene            complement(2075..2479)
                     /locus_tag="VGP27_14775"
     CDS             complement(2075..2479)
                     /locus_tag="VGP27_14775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV7852597.1"
                     /translation="MATVFTQIINGDLPGRFVYEDDDIAAFLTIQPMTQGHTLVVPRA
                     EIDKWQDVDSAAFGRVMEVSQLIGKAVCKAFGAERAGMIIAGLEVPHLHVHVFPAYNL
                     TDFGFAHVDQNPSPESLDEAQSKIKAALADLT"
     gene            2553..3311
                     /locus_tag="VGP27_14780"
                     /pseudo
     CDS             2553..3311
                     /locus_tag="VGP27_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
     gene            complement(3373..3693)
                     /locus_tag="VGP27_14785"
                     /pseudo
     CDS             complement(3373..3693)
                     /locus_tag="VGP27_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3753..5051
                     /locus_tag="VGP27_14790"
     CDS             3753..5051
                     /locus_tag="VGP27_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7852598.1"
                     /translation="MFSETRYALNGDLRVAYRASAEGDRDIVFVPNWFTNCEVFPELP
                     SLQGWVEAMTSLGRLIFFDQPGNGASDPVTSAALPTLEQWADSITAVLDDLGSRQAVL
                     LVSSGTIATGALFAATHPSRTVALVVLEGYTGQAVPEEIPASFIAAWGTGEMEHAANP
                     DMPWNEEIRAAWARHERLAASPATFGLLFRLVLELDVRAILPTIRVPTLVVHHADDAL
                     VLPEYGKDVAHHIPGAKYVELPGRNTYHFVEPWRESFQEIAEFLTGQQADFADDRVLA
                     TLLFTDIVDSTHRAAEMGDRDWHALLDAHDAVVRSQLARFRGREVNTSGDGFLAMFDG
                     PQRAIRCAMAIRDAVQALGIEVRAGLHTGECEVRGDDIGGIGVHIGARVSALAGPNDV
                     LVSSTLRDLVIGSGLEFEDRGTHQLKGVPGEWHISAVASR"
BASE COUNT          833 a         1646 c         1700 g          909 t
ORIGIN      
        1 gccgcgctcg acaaggcccg tgaagtgcgg cccgacgctg tcatcctcga cgtgatgatg
       61 cccgggatgg acggcttcgg cgtgctccgc aggctgcgcg ccgacggcat cgactcgccc
      121 gcgctgttcc tcaccgcccg cgacaccctc caggacaaga tcgcgggcct gacactcggc
      181 ggcgacgact acgtcaccaa gcccttcagc ctggaggaag tcgtcgcccg gctgcgggtg
      241 atcctgcgcc ggtcgggccg gggtaccgag gagccacgca gcgcgaagct gaccttcgcc
      301 gacatcgaac tcgacgagga cacccatgag gtgtggaagg ccggggagcc ggtttcgctg
      361 tcgccgaccg agttcacgct gctgcggtat ttcatcatca acgcaggcac ggtgctgtcc
      421 aagccgaaga tcctcgacca cgtctggcgc tacgacttcg gcggcgacgt gaacgtcgtc
      481 gagtcgtacg tctcctacct gcgccgcaag atcgacaccg gcgagaagcg ccttctgcac
      541 accctgaggg gcgtcggcta cgttttgcgg gaaccgcgat gagcgcttgc cgtgaagagc
      601 cgagaactca gctagattct cctgaacaat aggagtatgg cgggtctgcg cggacgaggt
      661 gtgccgctgc gggtcgggct ggtggccgcg accctcgtat tggtggcgtg cggtttgttg
      721 gcgtccggca tcgccgtcac ctcgatactt cggcacagcc tgacaaaccg catcgacgag
      781 acactgctcg acgcctcacg tagctgggcg caggcgccgc gtcgcatgcc ggtgcctccg
      841 atggagccca acccggcccg tccgccgtcg gacttctacg tgcggggggt cgatcccgac
      901 ggccggatct ggatggccgt caacgatcgt gcggctgaac cggcactgcc ggcgaacaac
      961 gacgtcggtc cggcgccggt caccgtcggc tcgatcgaca attcgcacgt gcagtggcgt
     1021 gcgatgtcgg tgcacgggcc gcaggatcag atcacgaccg tggccatcga tctgtccgat
     1081 gtccagtcga ccgttcgcgc gctgatctgg tcgcaggccg gcatcggcgc tgccgtgctg
     1141 ctggtgcttg gcgtcgccgg tttctgggtg gtgcaccgca gcctgcggcc gttgaccgaa
     1201 gtcgaacaga ccgccgccga catcgccgcc ggacagctgg atcgccgtat cccacaacga
     1261 gatccgcgta ccgaggtggg ccgcttgtcg ttggcgctca acggcatgct ggcgcagatt
     1321 cagcgcgctg tcgcgtcatc ggaatcctcc gcggaacagg cccgcagatc cgaggagcgc
     1381 atgcgccgct tcatcaccga cgccagccac gagctgcgga cgccgctgac cacgatccgt
     1441 ggtttcgcgg agctgtaccg ccagggcgcc gcccgcgaca tcgagatgct gatgtcgcgt
     1501 atcgagagcg agtcacgccg gatggggttg ctggtggagg atcttctgct gctcgcccgc
     1561 ctcgacgcgc agcgaccact ggatcgtcac cgcgtcgacc tgctggcgct ggccaccgac
     1621 tcagtgcacg acgcgcggtc gatcgccgcg aaacgcaaaa tcaccgtgga ggtcatcgac
     1681 ggtcccggca cgccggaggt gctcggtgac gaggcacggt tgcgccaggt actgggcaat
     1741 ctcgtcgcca acgcgctgca gcacacgccg gaaaccgcgc gaatcaccgt gcgtgtcggc
     1801 accgacgacg acaacgccct actcgaggtc gccgacgagg gtcccggcat gactcccgag
     1861 gacgcccacc gtgtcttcga gcggttctac cgcaccgacg agtcccgcga ccgggcaagt
     1921 ggcggcaccg ggctggggct gtcgatcgtc gactcgctgg tctacgccca cggcggctcg
     1981 gtcagcgtca ccaccgcgcc gggcaggggt tcccggttca ccgtcaagtt gccgcgcatc
     2041 gcggatgccg tcacgcacga tgtcgcggcg tcggtcaggt caggtcggcc agcgccgcct
     2101 tgatcttgct ctgcgcctcg tcgagtgact ccggcgacgg attctggtcg acgtgggcga
     2161 acccgaaatc ggtgaggttg taggccggga agacgtgcac gtgcaggtgg ggaacctcaa
     2221 gcccggcgat gatcatgccc gcccgctcgg cgccgaatgc cttgcacacc gccttgccga
     2281 tcaattgcga gacctccatg actctgccga acgcggccga gtcgacgtcc tgccacttgt
     2341 cgatctccgc ccgcgggacg acgagcgtgt gcccctgcgt catcggctgg atcgtcagga
     2401 acgccgcgat gtcatcgtcc tcgtagacga accggccagg caaatcgccg ttgatgatct
     2461 gggtgaagac ggtagccatg gttccgagca ttccagcaag ctcccagtcg cgcggcagtg
     2521 gggtggcagc tcgctcgtag acggttatcg atatgacgga tccggcggaa tcgctcgccg
     2581 actacgtgct ggacatcgtc attccggtcc acaacgaggt gcgcgacctt cccggctgca
     2641 tccaccgtct acatgattat gtcggcgcct acgtgcccta ctcgacgcgg atcacggtgg
     2701 ccgacaacgc aagtacggac gacacactca acgttgcgca cacgctcgcc gacgaactcc
     2761 ccgacgtcga ggtcatccat atcgatgcga agggccgcgg cgcggcgctg cacgccgcgt
     2821 gggcgaaatc gcctgcggac gttgtcgcct acatggacgt cgacctgtcc acgggcctgt
     2881 cggcgtttat gcccctcgtc gcgccggtga tctcaggcca ttccgagctg gcgatcggct
     2941 cgcggttgac ttcgtcgtcg cgagtcatcc gaagtgccaa gcgtgagttc gtttcgcgca
     3001 gttacaactt gctgctgcgc gcggctctcg gtgcacgctt ttccgacgct cagtgcggct
     3061 tcaaggcggt gcgcgcagag gtcgcccgcg aactgcttcc attcgtcggc gacaccggct
     3121 ggttcttcga caccgaactg ctggtcatcg ccgaacgagt tggattacgg atccacgagg
     3181 tgcccgtcga ctggatcgaa gaccccgact cacgggttga catcgtgcag actgcgatcg
     3241 gtgatttgaa gggctgttgg cgactctggc gggcactcgt cacccgggcg ctgccgatcc
     3301 gcgaattgcg gcacagaatt ggccgcgcgc caaaggcggc gggggcgcct cgatgtgaaa
     3361 ggtcttcggc gcctaaaagg gcggtggctc gtccttgacg gcagacatcg cgatcgcctc
     3421 ttcccagcgt cgtctgccga gctcggtctc gcggtgttgt cggttgagcc tgcgttcggc
     3481 aacgatgcgc agtgctcggt tctgcgcccg cttgcgactg cgtgtgggca tcatcgtggc
     3541 gcggtccgtg cattcccgag ctggggtggc ggggacgggg agtgctccgg tcggtgcgca
     3601 tagcgacgga aacagcagcg cgctaccggg tgtggtgaca taggtttcgc ctgacgggga
     3661 cgtccagatg acagtgccgt cgagtcgttg gcgataagcc aagcggcatg actccggtaa
     3721 tcgacgcgcg ttcctcggct tagacttccg ccatgttctc cgagacgcgg tatgcgctca
     3781 acggggactt gcgcgttgca tatcgggcgt cggctgaagg tgaccgggat atcgtgttcg
     3841 tcccgaactg gttcaccaac tgtgaggtct tcccggagct gccgtccctt caagggtggg
     3901 tcgaggccat gacgtcgctc gggcgactga tcttcttcga tcagccgggc aacggagcat
     3961 ccgatccggt cacatctgcc gcgctgccga ctttggagca atgggccgac agcatcaccg
     4021 cggtgttgga cgatctcggg agtcgccaag cggtgctctt ggtgagcagc ggcacgatcg
     4081 cgacgggcgc gctgttcgcg gcaacgcatc cgtcccggac agtcgcgctg gtcgtgctag
     4141 agggctacac cgggcaagca gtgcccgagg aaatcccggc ttcgttcatt gccgcgtggg
     4201 gcacggggga gatggagcat gcggccaacc cggacatgcc gtggaacgag gagattcggg
     4261 cagcatgggc gcgacatgaa cgcctggcgg cgagcccagc gaccttcggt ctcctgtttc
     4321 gtctcgtgct cgaattggac gtgcgggcga tcctcccgac catccgcgtg ccaaccctcg
     4381 tcgtccacca cgccgacgac gcgctggtgc tgcccgagta tggcaaggac gtcgcccatc
     4441 acattccggg cgcgaaatac gttgagctgc cagggcgcaa cacgtaccac ttcgtagaac
     4501 cgtggcgcga gtccttccaa gagatcgccg aattcctcac cggccagcag gctgacttcg
     4561 ccgacgaccg cgttctcgcc accttattgt tcactgacat tgtcgactcg acacaccgcg
     4621 ccgcggagat gggcgaccgt gattggcatg cacttctcga tgcgcacgac gcggtcgtac
     4681 gctcgcagct tgcgcggttt cgaggtcgcg aggtgaacac atcgggagat ggcttcctgg
     4741 cgatgttcga cggtccgcag cgggcgatcc gttgcgcgat ggcgatccgc gacgcggtgc
     4801 aggcgctcgg catcgaggtg cgcgccgggt tgcataccgg tgagtgcgag gtgcgcggtg
     4861 acgatatcgg cggtatcggc gtgcatatcg gcgcgcgggt gagcgcattg gcagggccga
     4921 acgatgtcct ggtgtccagc acactgcgcg acctggtcat cgggtcgggg ctcgagttcg
     4981 aggaccgcgg aactcaccaa ctcaaagggg tacccgggga atggcacata tccgccgtcg
     5041 catctcggta gcggcagccg cactgagggt gtgtcctaca acaatgac
//
LOCUS       DASYLX010000623         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_909514,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000623 DASYLX010000000
VERSION     DASYLX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909514"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..940)
                     /locus_tag="VGP27_14795"
     CDS             complement(239..940)
                     /locus_tag="VGP27_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEV7852599.1"
                     /translation="MTLVLTAHGSADARSAASVCELAGTVRRVRRGVDVRVAFCEQNS
                     PNLRDVLASAGRDAVVVPLLLADAYHARVDIPAMIRDSGSSARQADVLSEDDRLVAVL
                     RQRLTHAGVSRLDPSVGVLVTAVGSSRPQANARTAAVADYLVPHTNWTATTAFATGPH
                     PTLPEAAEVLRDRGATRLIIAPWFLAHGRITDRVAVFARAHGMSMAEPLGPHRLVAET
                     VLDRFDAVAAARRVA"
     gene            complement(937..1629)
                     /locus_tag="VGP27_14800"
     CDS             complement(937..1629)
                     /locus_tag="VGP27_14800"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEV7852600.1"
                     /translation="MTETDLQRLAERGAAELEGAGAADLLRWTDENFGGDYVVASNMQ
                     DAALVDMAAKVRPGVDILFLDTGYHFVETIGTRDAVEAVYDVNIVNVTAEKSVARQDQ
                     LFGKNLFAREPNECCRMRKVEPLGRALQNYSAWVTGIRRVEAPTRANAPLISWDKAFN
                     LVKINPIAAWTDEDMQAYIDSNDVLVNPLVFEGYPSIGCAPCTAKPVEGADPRSGRWA
                     GTAKTECGLHAS"
BASE COUNT          286 a          554 c          566 g          283 t
ORIGIN      
        1 tcctgttgcg cagacaaagc gaagagggtt cagccacaac aggagtcatc agagggcgcg
       61 gtagtcaccc gatattggta ccggctggga gttgtctgga ggatattcag gctcagcgag
      121 aatttattgc cggctgcgaa agctggctga gcagcgacga agaacggccg aatgtgcggt
      181 ttcgtccgca acactcccgg atgagcgtgg gaaagcgctc cctcgaagcg agtgagtact
      241 acgcgacgcg gcgtgcagcc gcgacggcgt cgaagcgatc aagcacggtc tcggcgacca
      301 accggtgcgg accgagtggc tctgccatcg acatcccgtg tgctcgcgcg aagaccgcga
      361 cgcggtcggt gatccggcca tgggcgagga accacggcgc gatgatcaat cgggtcgcgc
      421 cacggtcacg cagaacctcg gcggcttccg gaagtgtggg atgcggtccg gtggcgaaag
      481 cggtggtcgc cgtccaattg gtatgcggga ccagataatc ggcgaccgcg gcggtgcgcg
      541 cattggcctg cgggcgtgac gagcccactg cagtaaccag aacgccgacg ctgggatcca
      601 gccgggacac gcctgcatgc gtcagccgct gccgcagcac cgcgacgagg cggtcgtcct
      661 cgctgaggac gtcggcctgc cgcgccgacg accccgaatc gcggatcatc gcggggatgt
      721 cgacgcgggc gtggtaggcg tcggcgagca acagtggcac cacgacggcg tcgcggccgg
      781 cggacgcaag gacgtcacgg agattggggg agttctgttc gcagaacgcc acccgcacat
      841 cgacgccccg gcggaccctt cggacggtgc ccgcgagttc gcacaccgac gccgcggacc
      901 tcgcgtccgc gctgccgtgc gccgtcagga cgagtgtcac gaagcgtgca gcccgcattc
      961 cgtcttggcc gtgcccgccc agcggccgct gcgcggatcg gcgccctcga ccggtttggc
     1021 cgtgcacggt gcgcatccga ttgacggata gccctcgaac acaaggggat tgaccagaac
     1081 gtcgttgctg tcgatatagg cctgcatgtc ttcgtcggtc cacgccgcga tgggattgat
     1141 cttgaccagg ttgaaggcct tgtcccaact gatcaacggc gcgttggccc tcgtcggcgc
     1201 ctcgacccga cgtatcccgg tcacccaggc cgagtagttc tgcaaagccc tgcccaaagg
     1261 ttcgaccttg cgcatccggc agcactcgtt gggctcgcgg gcgaacaggt tcttgccgaa
     1321 cagctggtcc tgcctggcga cgctcttctc ggccgtcacg ttgacgatgt tgacgtcgta
     1381 aacggcttcg acggcgtcgc gggtgccgat ggtctccacg aagtggtagc cggtgtccag
     1441 gaacaggatg tcgacgcccg gccgcacctt ggccgccata tcgacgagcg cggcgtcctg
     1501 catgttcgac gcgaccacat aatcgccgcc gaagttctcg tccgtccagc gcagcaggtc
     1561 cgccgcgcct gcgccctcga gttcggccgc gccgcgttcg gccagccgct gcaagtccgt
     1621 ctcggtcatc gtcacctcag tgctcgtctc ttcgcgcaag cgctcatcga aggtccgcct
     1681 cgtctgctc
//
LOCUS       DASYLX010000624         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1128473,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000624 DASYLX010000000
VERSION     DASYLX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128473"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VGP27_14805"
     CDS             <1..579
                     /locus_tag="VGP27_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852601.1"
                     /translation="RDERRGQLLIAASEVFVDRGYHAAGMDEIADRAGVSKPVLYQHF
                     SSKLELYLAVLARHVDNLVSGVRQALRTTTDNRQRLRAAVSAFFDFIEQDGQGYRLIF
                     ENDYVTEPQVAGQVKVATESCTDAVFDLISRDSGLEAHRARMIAVGLVAVSVDSARYW
                     LNNERPISKDDAIEGTVQFAWGGLSHVPLTRS"
     gene            complement(566..817)
                     /locus_tag="VGP27_14810"
     CDS             complement(566..817)
                     /locus_tag="VGP27_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEV7852602.1"
                     /translation="MEVKIGVTDSPRELSFNSAQTPTEVEKQITDALATDSGVLALTD
                     EKGRRFLVQSAKITYVEIGAADVRRVGFGVVGAEAVKNG"
     gene            997..1737
                     /locus_tag="VGP27_14815"
     CDS             997..1737
                     /locus_tag="VGP27_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7852603.1"
                     /translation="MNRPSSPWMTGSVTDRSHDRPDSDDELADAEDYDAYADYQHDDY
                     DDLDDDVRYYTEQDDRRWLWVASVAGIVLLIAVAGTMMILSGNDSGTTTATVSPETSA
                     TAPYPSAAPSPSVRRAPSATALPPETITSVTPTPTAAPPSAVAEPTEAAPPPAGDPRT
                     VTYTVTGNRQLFDLVTIIYTDQQGALQTDVNVALPWTKQVTLDPGVQLSSVTATSVGG
                     QLSCSITDGSGATLASQANSTMIANCTR"
     gene            complement(1743..>1908)
                     /locus_tag="VGP27_14820"
     CDS             complement(1743..>1908)
                     /locus_tag="VGP27_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylase"
                     /protein_id="HEV7852604.1"
                     /translation="LLGEAITQAQFVLADRDELVDLVVSGTDGLGQLTQFFDRLQHTH
                     STRMQELGLA"
BASE COUNT          341 a          661 c          600 g          306 t
ORIGIN      
        1 cgcgatgagc gccgcgggca gttactcatc gctgccagcg aggttttcgt cgatcgcggc
       61 taccacgccg cggggatgga cgagatcgcg gatcgcgcgg gcgtcagcaa acctgttctc
      121 tatcaacact tttcgtcgaa gctggagctg tacttggcgg tgctggcccg ccatgtcgac
      181 aacctggtgt ccggtgtgcg ccaggcactg cgcaccacca ccgacaaccg gcagcggctg
      241 cgcgccgcgg tgtcggcgtt cttcgatttc atcgagcagg acggccaggg ctaccggttg
      301 atcttcgaaa acgactatgt caccgagccg caggtcgcgg ggcaggtcaa ggtcgcgacg
      361 gagtcctgca cggatgcggt tttcgacctg atcagccgtg attccggact cgaggcgcac
      421 cgcgccagga tgatcgcggt cggtctggtg gcggtcagcg tcgactccgc gcgatactgg
      481 ctgaacaacg aacggccgat ctccaaggac gacgcgatcg agggcactgt gcagttcgcc
      541 tggggcggac tgtcacacgt gcccctcacc cgttcttgac ggcctccgcg ccgaccacac
      601 cgaagccgac tcgccgcacg tcggcggcgc cgatctcgac ataggtgatc ttcgccgact
      661 gcaccaggaa ccgacgaccc ttttcgtcgg tcagcgcgag cacacccgaa tccgtggcca
      721 gcgcgtcggt gatctgcttc tctacctcgg tgggagtctg cgcgctgttg aagctgagct
      781 cgcgcgggct gtccgtgaca ccgatcttga cctccacgct gcaccccttc tgaagacgat
      841 tgcgtctgcg aaacgttctt aggagaaagg ctagtggacc cgtcacggtg aactgagtcc
      901 ggaccgttac caacacgcgg aacgctaaac ctcatctgtc acttctacct cgatagcatc
      961 aggcctgcac cactcgaact gacgaggatt acgcgcatga acaggccttc ttcgccctgg
     1021 atgaccggat cggtcaccga ccgcagtcac gaccgtcccg actcggatga cgagctcgcc
     1081 gacgccgagg actacgacgc gtacgccgat taccagcacg acgactacga cgacctcgac
     1141 gatgacgtcc ggtactacac cgagcaggac gaccggcgct ggctgtgggt ggccagcgtc
     1201 gcgggcatcg tgctgctcat cgccgttgcg ggcacgatga tgatcctcag cggaaacgac
     1261 agcggtacca cgaccgcgac ggtctcgccc gagaccagcg ctaccgcgcc ctatcccagc
     1321 gccgcgccgt cccccagcgt ccgcagggcg ccgtcggcca ccgcgctgcc gcccgagacc
     1381 atcaccagcg tgacgcccac gccgacggcc gcgccgccaa gtgccgtcgc cgagccgacc
     1441 gaagccgcgc cgccgcccgc gggcgacccg cgcacggtga cctacaccgt gaccggcaac
     1501 cgtcagctgt tcgatctggt cacgatcatc tacaccgacc agcagggcgc actgcagacc
     1561 gacgtgaacg tcgcactgcc gtggaccaag caggtgacgc tggatccggg ggttcagctc
     1621 agctccgtca ccgcgaccag cgtgggcggg cagctcagct gctcgatcac cgacgggtcc
     1681 ggcgcgacac tggcttcaca ggccaacagc acgatgatcg ccaactgcac ccgctgagcg
     1741 ggtcaggcca ggccgagctc ctgcatccgg gtgctgtgcg tgtgctggag gcggtcgaag
     1801 aactgcgtca gctgacccag cccgtcggtg cccgagacga ccaggtcgac gagttcgtcg
     1861 cggtcggcga gaacgaactg cgcctgggtg atggcttcgc cgagcagc
//
LOCUS       DASYLX010000625         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1347497,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000625 DASYLX010000000
VERSION     DASYLX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347497"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VGP27_14825"
     CDS             complement(<1..1388)
                     /locus_tag="VGP27_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEV7852605.1"
                     /translation="MSRVLAIARNTWRTLTSMGTALVLLFLLALAAIPGALLPQRSLN
                     EAKVEQYIAEHPTIGPWLDRLQAFEVFSSFWFTAIYVLLFVSLVGCLTPRLAEHIKSM
                     RATPVAAPRNLSRLPKHHTAEVAGERDAVAAAVSDKLSGWRRVTRTEDDAVEISAEKG
                     YLREFGNIVFHFALLGLLVSVAVGKLFGYEGNVIVVADGGPGFCSASPAAFDSFRAGN
                     TVDGTSLSPICLRVNDFDAHYLPSGQALSFAANIDYQAGADLDSDTWRAYHLKVNEPL
                     RIGGDRIYLQGHGYAPTFTVTFPDGQTRTQTVQWRPDDQVTLMSSGVMRFDPPGGTYP
                     NADERRKHQIAIQGLFAPTEQLHGTLLSSSFPALNDPAVAVDIYRGDAGLDSGRPQSL
                     FSLDPRLIDQKRLTKAARVNLPAGEETRLDDGTRVRFDGAVPFINVQVSHDPAQVWVL
                     VSALTMMAGLLVS"
     gene            complement(1385..>1614)
                     /locus_tag="VGP27_14830"
     CDS             complement(1385..>1614)
                     /locus_tag="VGP27_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV7852606.1"
                     /translation="VIAYCLGLGIPFVLLAFGSAGAVQGLGWLRRHTRQIQIFGGVLL
                     ILVGTALVTGVWNDFVSWVRDAFVSDVRLPI"
BASE COUNT          288 a          538 c          557 g          231 t
ORIGIN      
        1 gagaccagca ggcccgccat catcgtcagc gcggacacca gcacccacac ctgtgccgga
       61 tcgtgggaga cctgcacgtt gatgaacggc accgccccgt cgaagcgcac ccgcgtgccg
      121 tcgtccagcc gggtctcctc gccggcgggc aggttcaccc gtgccgcctt ggtcagccgc
      181 ttctggtcga tgagccgcgg gtccagcgag aacagcgact gcggccggcc gctatcgagt
      241 cccgcgtcgc cgcgatagat gtcgacggcc accgcgggat cgttgagcgc agggaagctc
      301 gacgacaaca gggtgccgtg cagctgttcc gtcggcgcga aaagtccctg gatcgcgatc
      361 tggtgcttgc ggcgttcgtc ggcgttcgga taggtgccgc cgggcgggtc gaaccgcatc
      421 acgcccgacg acatcagcgt gacctgatcg tccggccgcc actgcaccgt ctgggtgcgg
      481 gtctgcccgt cggggaaggt gacggtgaac gtcggcgcgt aaccgtgccc ctgcaggtag
      541 atccggtcac caccgatgcg cagcggttcg ttgaccttga ggtggtacgc ccgccaggtg
      601 tcgctgtcga ggtcggcgcc cgcctgataa tcgatgttgg cggcgaagga cagcgcctgc
      661 cccgacggca ggtagtgggc gtcgaagtcg ttgacgcgca agcagatcgg gctcagcgag
      721 gtgccgtcga cggtgtttcc cgcgcggaac gagtcgaagg cggcaggcga cgccgagcag
      781 aagcccggcc cgccgtcggc gaccacgatg acgttgccct cgtagccgaa aagcttgccg
      841 acagccaccg agacgagcag gccgagcagg gcgaagtgga agacgatgtt gccgaactcg
      901 cgcagatagc ccttctcggc ggagatttcg acggcatcgt cctcggtgcg tgtgacgcgt
      961 cgccacccgc tcagcttgtc cgataccgcg gcggcgaccg cgtcgcgctc gcccgccacc
     1021 tcggcggtgt ggtgtttggg cagccgactg aggttgcgcg gcgcggcaac gggggtggcc
     1081 cgcatgctct tgatgtgctc ggccagcctc ggggtcaggc agccgacgag ggagacgaac
     1141 agcaacacgt agatggcggt gaaccagaag ctggagaaca cctcgaaggc ctgcaaccga
     1201 tccagccagg gcccgatcgt cggatgctcg gcgatgtact gctcgacctt tgcctcattg
     1261 aggctgcgct gcggcagcag ggcgcccgga atggcggcca gcgcaagaag gaacagcagc
     1321 accagcgccg tgcccatcga cgtcagcgtg cgccacgtgt tgcgggcaat cgccagaacg
     1381 cggctcatat cgggagcctc acgtcgctga cgaacgcgtc gcgcacccac gacacgaagt
     1441 cgttccacac gccggtgacc aacgcggtgc cgaccaggat cagcagcaca ccgccgaaga
     1501 tctggatctg ccgggtgtgc cggcgcagcc agccgaggcc ctgcacggca cccgccgaac
     1561 cgaacgccaa cagcacgaac gggatgccca agccgaggca ataggcgatc acca
//
LOCUS       DASYLX010000626         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_580982,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000626 DASYLX010000000
VERSION     DASYLX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580982"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VGP27_14835"
     CDS             <1..816
                     /locus_tag="VGP27_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEV7852607.1"
                     /translation="ALHEPVTLLVLPRLDAWTALDLIERERATGIVGWPAFTQRLRED
                     PSFATRDLSSAPMLRDGPLDVAMTDVPDGFPVHRTMSETAGGFAFTQMRIVDDNGDRV
                     TDGTVGELLIRGIGVMSGYNKRERSETFDDDGWYHTGDQVYRKRDDPRLFYVGRTTEL
                     IKAAGANVSPLEVEGVIEQFTEVAQCVVIGIDDPVRGEEVCAVVVPAVAEIDLESMAT
                     RARDLLSAYKVPTRWVLAASDRIPVLPSGKLNRKGLRALVGDGELAAVRTMSQ"
     gene            complement(810..>2036)
                     /locus_tag="VGP27_14840"
     CDS             complement(810..>2036)
                     /locus_tag="VGP27_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7852608.1"
                     /translation="DNAYFPLLMPMSFFEREAEHVEGFSPELAVVTHGGGKELAEPLA
                     IRPTSETVVGEYMAKWVDSYRDLPLLLNQWANVVRWEMRPRIFLRTTEFLWQEGHTAH
                     ATQSDARRYARLILHDVYEDFMRSVLAMPVVVGRKTANERFAGAANTMTCEGIMRDGK
                     ALQMGTSHELGQRFSRAFDISYLNAEGAREFCWTTSWGSSTRMLGGLIMCHGDDFGLV
                     LPPALAPIQAVVVVVKDIDGSVTRAASTVLEELARRGVRTRLDDNTDQGFGRRATEWD
                     LRGVPIRVEIGPRDVADGCALLYRRDTRDKATVALDSVVDEAHRLIRRIQTDMLSAAT
                     ARRDAFIVECTTIEQVAHVAQTGVARVPWVSVGVSGEQRLAADGVMVRCLQRGDGGLP
                     DTDDDTDVMAYVARAY"
BASE COUNT          341 a          669 c          681 g          345 t
ORIGIN      
        1 gcgctgcacg aacccgtcac gctgttggtg ctcccccggc tcgacgcttg gaccgcgctg
       61 gacctgatcg agcgtgagcg ggcgacgggg atcgtagggt ggccggcgtt cacccagcga
      121 ctgcgcgagg atccgtcatt cgcgacccgt gacctttcca gcgccccgat gctgcgcgat
      181 gggccgttgg acgtcgcgat gaccgacgtg cccgacggat ttccggtgca ccgcaccatg
      241 tccgagacgg cgggcgggtt cgctttcacg cagatgcgca tcgtcgatga caacggcgac
      301 cgggtgaccg acggcaccgt gggcgagttg ctcatcaggg gcatcggggt gatgtcggga
      361 tacaacaagc gtgagcgttc ggagacgttc gacgacgacg gctggtacca caccggcgac
      421 caggtctacc gaaaaaggga cgacccgcgg ctgttctacg tgggccgcac caccgaactc
      481 atcaaagccg caggcgcgaa cgtgtcacct ctggaagtcg agggggtgat cgaacagttc
      541 accgaggtgg cacagtgcgt ggtcatcggc atcgacgatc ctgttcgggg cgaggaggtg
      601 tgcgccgtcg tcgtccctgc cgtcgccgag atcgacctcg aatcaatggc tacgcgtgcg
      661 cgtgacctgt tgtcggccta caaggttccg acgcgttggg tactggcggc gagcgaccgg
      721 ataccggtcc tgccaagcgg aaagttgaac cgaaaagggc tgcgcgccct tgtcggcgac
      781 ggggaactgg cggcggtccg gaccatgagt cagtaagccc gcgcgacgta cgccatcaca
      841 tccgtgtcgt cgtcggtgtc gggaagtcct ccgtcaccgc gctgcaggca ccgcaccatg
      901 acgccgtcgg cggccaggcg ctgttcaccg gagacaccga ccgataccca cggcacgcgg
      961 gcgacgccgg tctgcgcaac gtgggccacc tgctcgatgg tggtgcactc gacgatgaag
     1021 gcgtcgcgtc gcgccgttgc ggcgctgagc atgtcggtct gtatgcgccg gatcagccga
     1081 tgtgcctcgt cgacaaccga atcgagtgcc accgtggcct tgtcgcgggt gtcgcgccgg
     1141 tagaggaggg cgcagccgtc ggcgacgtct cgcgggccga tctccacccg tatcggcacg
     1201 ccccggaggt cccattcggt ggcgcgccgg ccgaaaccct gatcggtgtt gtcgtccagc
     1261 cgtgtccgca cgccgcgccg tgccaactcc tcgagcacgg tcgacgccgc gcgggtgacc
     1321 gagccatcga tgtccttcac caccaccacg acggcctgga tcggtgccag tgccggcggc
     1381 aggacgagcc cgaagtcgtc gccgtggcac atgatcagcc caccgagcat gcgggtcgac
     1441 gacccccacg acgtcgtcca gcagaactcc cttgcgcctt cggcattgag gtagctgatg
     1501 tcgaaggcgc ggctgaagcg ctggcccagt tcgtgtgacg tgcccatctg cagcgccttg
     1561 ccgtcgcgca tgatgccttc gcacgtcatc gtgttggcgg cgcccgcgaa gcgctcgttc
     1621 gcggtcttgc ggccgacgac aacgggcatg gcgagcacgc tgcgcatgaa atcctcgtag
     1681 acatcgtgca ggattaggcg cgcataccgc cgcgcgtcgg actgcgtcgc atgcgcggtg
     1741 tggccctcct gccacaggaa ttcggtggtg cgcagaaaga ttcgcggacg catctcccat
     1801 cgcacgacat tggcccactg gttgagaagc agcggcagat cacggtagct gtcaacccat
     1861 ttggccatgt attcgccgac gacggtctcg ctcgtcggac gaatggcgag cggttcggcc
     1921 agctccttgc ctccgccgtg cgtcaccaca gccaactccg ggctgaatcc ctcgacgtgt
     1981 tcggcttcgc gctcgaagaa actcatcggc atcagcaacg ggaagtacgc gttatc
//
LOCUS       DASYLX010000627         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2336160,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000627 DASYLX010000000
VERSION     DASYLX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336160"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..575
                     /locus_tag="VGP27_14845"
     CDS             3..575
                     /locus_tag="VGP27_14845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7852609.1"
                     /translation="MEPLSRGHVPEIAAASADGDVGGLWFTGAPSPQTASQWVDMRLG
                     LQTPETGLTFVVRRLDDTADARRDQGAVVGSSSYLNVDAPNRRVEIGNTWYGASARRT
                     GINTETKLLMLGHAFDVLNCVAVEFRTHFFNFTSRTAIERLGAKLDGILRSHQLVPDG
                     TRRDTVVYSILDVEWPTVATHLKFRLDRHG"
     gene            complement(578..3862)
                     /locus_tag="VGP27_14850"
     CDS             complement(578..3862)
                     /locus_tag="VGP27_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV7852610.1"
                     /translation="MSEIDARPLNWSDLGVSELVPTGTVTLLLADVEGSTRLWERQPA
                     EMTAAFASLDRTLAELVGVHGGVRPVEQGEGDSFVIAFGRPSDAVACALDLQRAPLAP
                     IQLRIGLHTGEVQLRDESNYIGPTINRTARIRDLGHGGQTVLSGITTDLVCDRLPDAA
                     WLADLGTHPVRDLPRPERIVQLCHPDIRNEFPPLRTAKTTRSRNLPAQLTSFVGRAGQ
                     IDELRGLVSNNRLVTLTGAGGAGKTRLAVEVAAQLAHDFEDGVWWVDLAPVADPAVVT
                     LTVARTLGLPDQPGRSPLETVQRFIGDRKILLLLDNCEHLLDVCGDAITRLLNSCPRL
                     MIVTTSREPISVSGEVTWRVPSLSLHDEAIALFTDRAQRARPTFHAAGDDAELVAEIC
                     RRLDGMPLAIELAAARVRALSVRQIADSLKDRFRLLTGGARNVLRRQQTLRASVDWSH
                     ALLTETEQTLFRRLGVFMGGFDLDGARAVATTSEAEEFQILDQLSLLVDKSLVLADEE
                     SGGMRYGLLETVRQYALEKLAESGEADVVRNRHRDHYIATAVEMNGEDRLVEWAEQET
                     GNLRAAHAWSTDSAEFEPALRLASSLQRVWEKRGRMGEGMAAFDLVFSDKRYRDDDVA
                     PSVWARAVADKSILAAWTGTPASLQRGEEALAAARNLGDPALIAACLAACGALAYYSP
                     DVSKTYLTDAIDLSRASGDRSKLCYILSCLAVTMNIAGQPIASQLAAEEGRDVAEAVG
                     DGFVSRHCRVWLAIALAWRGGVTAADAVLRGLSEEAHAAGERMLELFALVCESNSSAW
                     QGNAAAARAKTDQARETSAALGGFHEDSVYATAAYAALAAGDAAAAKAACESAMAHAV
                     PQRELFMRATTPMTDALLGCEEVTAARRWADDTVVMTVGNYQMFALVARAYVAMAQGE
                     NDQAERDAHDALALAADTGAFMRVPEALEVLGRSACAEGNHRHAPRLFGAAEAIRAAM
                     GVVRWPVYSRGYDAAVALVRESLGDGDFDAAWAEGAALSIDEAIAYVQRGRGERKRPA
                     RGWEALTPTERDVVRHAADGLGNNDIAGRLFVSPRTVQTHLTHVYAKLGLTSRIQLIK
                     EAARHV"
     gene            complement(4012..4458)
                     /locus_tag="VGP27_14855"
     CDS             complement(4012..4458)
                     /locus_tag="VGP27_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7852611.1"
                     /translation="MLRFDPFSDLDAVTRGLLTSQTGSNRTPRFMPMDLCKIDDHYVL
                     TADLPGIDPGSVDVDVDNGTLTIAARRTARSDESVQWLTSERFFGSYRRQLSLGEGID
                     TTAISATYENGVLTVTIPMAERAKPRKIDIAHGGDQKSIPTTIDSE"
     gene            complement(4632..5483)
                     /locus_tag="VGP27_14860"
     CDS             complement(4632..5483)
                     /locus_tag="VGP27_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV7852612.1"
                     /translation="MLGQLGDEWTLLVIRQALMGATRYADFQGRLPISHSVLSSRLRS
                     LTRDGLLIKRAYQTNPPRAEYLTTPRSRSLWPVLVSIWDWERRWVPGHAGGLPAMHHT
                     TCDANFAPQLTCRSCGDAVADKDVVAQWGPSGSWARSVPLAATRRRSSSDPRKAGLFP
                     QTMSVLGNRWGFALLVAAFVGMSRFTDFQTQLAAPPGSIADRLSILVSNGVMAMADNR
                     YRLTEKGRALFPVLISALQWAQRWFHGPEGPAVVLTHTACGGRFTAVMTCDQCTLPLR
                     GTLITAV"
     gene            complement(5547..>6004)
                     /locus_tag="VGP27_14865"
     CDS             complement(5547..>6004)
                     /locus_tag="VGP27_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEV7852613.1"
                     /translation="DSITEFGMINANYAGRHYRYTYAATGKPGWFLFDGLVKHDVQTG
                     SEDRYAFDDGVYGSETAMAPRSDVAGRAAPGSGGTAEDDGYLVTLTTDMNDDASYCLV
                     FDAARVADGPVCKLRLPERISSGTHCTWADGSSLRRWHTADTPSAAIGL"
BASE COUNT         1017 a         1984 c         1957 g         1046 t
ORIGIN      
        1 cgctggaacc ccttagccgt ggccatgttc cggagatcgc tgcggcatcg gccgacggcg
       61 acgtcggcgg cctgtggttc accggtgcgc cgtcgccaca gaccgcgtcg cagtgggtgg
      121 acatgcggct cgggttgcag acccccgaga cggggctgac gttcgtggtg cgtcgcctcg
      181 acgacaccgc ggacgcgagg cgcgatcagg gagccgtggt cgggtcatcg agctacctca
      241 atgtcgacgc gccgaaccgg cgagtggaga tcggcaacac ctggtacggc gcgtcggccc
      301 ggcgcactgg catcaacacg gagaccaagc tgctgatgct gggccacgcg ttcgacgtat
      361 tgaattgcgt ggcagtggaa ttccgcacgc acttcttcaa cttcaccagc cgcacggcca
      421 tcgagcgact tggcgcgaag ctcgacggaa tcctgcgcag ccatcaactt gtgccggacg
      481 ggacgcggcg cgacaccgtg gtgtactcga tcctggatgt cgaatggcca acggtcgcta
      541 cgcacttgaa gtttcgtttg gaccgccacg gctgatgtca cacgtgtcgg gcggcctctt
      601 tgatcagctg gatgcgcgaa gtcagtccga gtttcgcgta cacatgtgtg aggtgagtct
      661 gcacggtacg cggtgacacg aacagccgtc cggcgatgtc gttgttgccg agcccatcgg
      721 ccgcgtgccg cacgacgtca cgttcggtcg gtgtcaacgc ttcccagccg cgcgccggtc
      781 gcttgcgttc cccgcgtccg cgctgaacgt acgcgatggc ttcatcgatc gacagtgcgg
      841 cgccttcggc ccacgcagca tcgaagtcac cgtcgcccaa gctttctcgg acaagcgcga
      901 cggcggcgtc gtagcccctc gagtacacag gccatcgaac gacgcccatc gcggcgcgga
      961 tggcctccgc agcaccgaag agccgcgggg cgtggcggtg attgccttcc gcgcacgctg
     1021 accgtccgag cacttcaagc gcttcgggca cgcgcatgaa tgcgcctgtg tcagctgcga
     1081 gtgccagggc gtcgtgggcg tcccgttcgg cctggtcgtt ttcgccctgt gccatcgcga
     1141 cataagcgcg cgcgacgagg gcaaacatct gatagttgcc gaccgtcatg acgacggtgt
     1201 cgtcggccca tcggcgtgcg gccgtgactt cttcgcaccc cagcaacgcg tcggtcatcg
     1261 gagtagtcgc ccgcatgaag agctcgcgtt gaggaacggc gtgtgccatc gccgattcgc
     1321 atgccgcctt ggccgcggcg gcgtccccgg cggccagcgc cgcgtaagcc gccgttgcgt
     1381 aaacactgtc ctcgtggaat ccgcccaacg ccgccgatgt ttcacgcgcc tggtccgtct
     1441 tcgcgcgagc cgctgcggca tttccctgcc aggccgacga gttgctctca cagacgagtg
     1501 cgaacaactc gagcatgcgc tcgccggccg cgtgggcctc ctcgctcaag ccccggagaa
     1561 cggcgtcggc tgcggtgacc cccccacgcc atgccagtgc gattgccagc cacacccgac
     1621 aatgccgcga tacgaacccg tcaccgaccg cctcggcgac gtcgcgcccc tcttcagccg
     1681 ccagttggga ggcgatcggc tgtcccgcga tattcatcgt gacagccagg cagctcagga
     1741 tgtaacacag cttcgaccga tcgcccgacg cgcgtgagag atcgatcgcg tccgtcaagt
     1801 acgtcttcga cacatcgggg ctgtagtacg ccagcgcacc gcacgcggcg aggcatgcag
     1861 cgatgagtgc cgggtcgccg agattccgtg cggcagccag agcctcttca ccgcgctgca
     1921 ggctagccgg tgtgccggtc catgcagcaa ggatgctctt gtcggcgact gcgcgagccc
     1981 agacggatgg cgctacatcg tcatcccgat agcgtttgtc gctgaagacg agatcgaacg
     2041 cggccattcc ttcacccatt cggccgcgtt tctcccacac ccgttgcagc gacgaagcca
     2101 gtcttaacgc gggctcgaac tcggcggaat cggtgctcca cgcatgcgcc gcgcgcaggt
     2161 tgccggtttc ttgttcggcc cattcgacta gccgatcctc gccgttcatc tcaacggcgg
     2221 tggcgatgta gtggtcgcga tggcggttac gcaccacatc cgcttcgccg gactcggcga
     2281 gcttttccag cgcgtactgg cggaccgtct cgagtaggcc gtagcgcatc ccgcccgatt
     2341 cttcgtcagc gagcacgagg gacttgtcaa ctagaaggct gagctgatcg agaatctgga
     2401 actcctcggc ctcgctggtg gtggcaacag cgcgcgcgcc gtccaggtcg aatccaccca
     2461 tgaaaacgcc gagcctgcga aacagcgttt gttcggtttc ggtgagcagc gcatgcgacc
     2521 agtccaccga agcgcgtagg gtctgctggc gccgcaacac gtttcgagcg ccgccggtga
     2581 gcaggcggaa cctgtccttg aggctatcgg cgatttgtcg cacggacagc gcgcgcaccc
     2641 gtgcggccgc caactcgatc gccagaggca tcccgtcgag ccgtcggcag atctcggcca
     2701 ccagttcggc atcgtcgcct gcggcgtgga aggtgggccg cgcgcgttga gccctgtccg
     2761 tgaacagtgc aatggcctcg tcgtgtagcg acagcgaggg cacccgccag gtgacctcac
     2821 ccgagacgga gatcggttcg cggcttgtgg tcacgatcat caaccgcggg cagctgttca
     2881 gcagcctcgt gatggcgtcg ccacagacgt cgagaagatg ttcacagttg tccagcagca
     2941 ggagtatttt acgatcgccg atgaaacgct gcaccgtttc cagcggcgat cgccccggtt
     3001 ggtcaggcag cccgagggta cgggccaccg tcagcgtgac gacggcggga tcggcaaccg
     3061 gcgccagatc gacccaccac acgccgtctt cgaaatcgtg ggccagctgg gcggctacct
     3121 ccacggccag gcgtgtctta cctgcgccgc cggctccggt caacgtcacg aggcggttgt
     3181 tgctcaccag cccacgcagc tcgtcgatct gtccggcccg tccgacgaaa gaggtcagct
     3241 gggccgggag gttacgagaa cgggtggttt tcgcggtgcg cagcggtgga aactcgttgc
     3301 ggatgtcggg gtgacagagc tgtactatcc gttcgggccg cggcagatcg cggacggggt
     3361 gtgtgcccag gtcggccagc cacgcggcgt ccggcagcct gtcgcacact aaatcggttg
     3421 tgatgccaga caacacggtt tgtccaccgt gccccagatc gcgtatgcgt gcggtgcggt
     3481 tgatggtggg gccgatgtag ttggactcat cacgcagctg gacttcaccc gtgtgcagcc
     3541 cgatgcgcaa ctggatcggt gccaagggcg ctcgttgcag gtcgagcgcg cacgccaccg
     3601 cgtcactggg ccgcccgaac gcgatgacga agctgtcgcc ctcgccttgt tcgacggggc
     3661 gtacgccgcc gtggacgccg accagttcgg caagtgtgcg atcgaggctc gcgaacgcgg
     3721 cggtcatctc cgcaggttgc ctttcccata gccgcgtcga accttcgaca tcggccaaca
     3781 gcaacgtcac cgtcccggtg ggtaccagct cgctcacacc aagatcgctc cagttcaacg
     3841 ggcgcgcgtc aatttcgctc atgctaacca gcctgcgacg agcgcacgcc gcaaacgtca
     3901 gcacagacgc gtagatctgt ggtgagaggg tgcgtaaatg tgcttcgaaa ctgcggacag
     3961 atcgcgtcgt cccactgatt cgcgatctgg ccgcagtctc gagcgcgtgg gttattcgga
     4021 atctatcgtg gtcggaatgc tcttctggtc gccgccgtgt gcgatgtcga tcttgcgcgg
     4081 cttcgcccgt tcggccatcg ggatcgtcac cgtcaacacg ccgttctcgt atgtcgccga
     4141 gatagcggtg gtgtcgatgc cttcgcccag cgatagttgt cgccggtagc tgccgaagaa
     4201 gcgttcgctg gtcagccact gaacggactc gtcgctgcgg gcggtgcgac gcgccgcgat
     4261 ggtcagcgtg ccgttgtcga cgtcgacgtc gacggaaccg ggatcgatgc cgggcaggtc
     4321 ggcggtcagc acgtaatggt catcgatctt gcacaagtcc atcggcatga accggggagt
     4381 gcgatttgat cccgtctggc tggtgagcaa gccccgggtc acggcatcaa ggtcactgaa
     4441 cggatcaaaa cgaagcacag caattcacct cctatagcgc ctctgaagcc cgccaccctg
     4501 gcgggcagcc aatcactgtg cgacagcgat attagcactc gcgccagggg agtgccagac
     4561 cgaaactgct gacagatcgc ttttcgcgcc gaaattgcga tctccccgca gtctcggcgg
     4621 aggcgtatcc gtcagaccgc cgtgatcagt gtcccgcgca agggcagcgt gcactggtcg
     4681 caggtcatga ccgcggtgaa gcgcccgcca cacgccgtgt gggtgagtac caccgccggc
     4741 ccctcgggac cgtgaaacca ccgctgcgcc cactgcagcg ccgagatcaa caccggaaac
     4801 agggcacggc ccttctcggt gagtcggtag cggttgtccg ccatggccat caccccgttc
     4861 gacaccaaga tcgacagtcg gtcggcgatg gagcccggcg gcgctgcgag ctgggtctgg
     4921 aagtcggtga accggctcat cccgacgaac gcggcaacca ggagtgcgaa cccccagcga
     4981 ttgccgagca cgctcatcgt ctgcgggaac aggcccgcct tacgcgggtc ggacgaggat
     5041 cgccgccggg tcgcggccag cggcaccgag cgcgcccacg atccgctcgg accccattgg
     5101 gcgacaacat ctttgtcggc gacggcatcg ccgcacgaac ggcaggtcag ttgcggggcg
     5161 aagttcgcgt cgcaggtcgt gtggtgcatc gcgggcaggc cgccggcatg cccgggtacc
     5221 caacgtcgtt cccagtccca gatcgacacc agaaccggcc acaacgaccg gctgcgcggc
     5281 gtggtcaggt actcggcgcg cggcggattc gtctggtagg cccgcttgat cagcagaccg
     5341 tcgcgcgtca gcgaccgcag tcggctcgag agcaccgagt gcgagatcgg caggcgtccc
     5401 tggaagtcgg cgtagcgggt tgcccccatc agtgcctgcc ggataaccag cagcgtccac
     5461 tcgtctccga gctgtccgag catccggccg accgcgttgg tatcgctgga caccaattgc
     5521 gttggccggg aggcggattg ctccacctag agcccgatcg cggcggacgg tgtgtcggcg
     5581 gtgtgccagc gccgcaacga cgatccgtcg gcccaggtgc aatgggtgcc actggaaatg
     5641 cgctccggca gccgcagctt gcacaccggt ccgtcggcca cccgggcggc gtcgaacacc
     5701 agacagtagg acgcgtcgtc gttcatgtcg gtggtcaggg tcaccagata gccgtcgtcc
     5761 tcggcggtgc cgccgctgcc cggagccgct cggccggcga catcggaccg cggtgccatc
     5821 gccgtctcac tgccatagac accgtcgtcg aacgcgtagc ggtcctcgga gccggtctgc
     5881 acgtcgtgtt tgaccaagcc gtcgaacagg aaccagccgg gcttgcctgt cgccgcatag
     5941 gtgtagcgat agtgccgacc cgcataattg gcattgatca tgccgaactc ggtgatgctg
     6001 tcgg
//
LOCUS       DASYLX010000628         7721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3324029,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000628 DASYLX010000000
VERSION     DASYLX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324029"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /gene="pepN"
                     /locus_tag="VGP27_14870"
     CDS             complement(<1..2213)
                     /gene="pepN"
                     /locus_tag="VGP27_14870"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEV7852614.1"
                     /translation="MVLPNLTRDQAIERAALVTVDRYGVDLDLTDGAGAPGEKTFRSI
                     TTVEFSALPGADTYIDIAADSVQRATLNGIDLDVSGYDESTGIALQGLQERNTLVVEA
                     LCLYSNTGEGLHRFVDPVDNEVYLYSQFETADAKRMFACFDQPDLKATFDIKVTAPAH
                     WEVVSNGAPADVQVAGGAKTLTFATTPKMSTYLVALIAGPYAVWRDAYTDEHGEIPLG
                     LYCRTSLAEYMDADRLFTETKQGFDFYHRNFGEPYAFGKYDQLFVPEFNAGAMENAGA
                     VTFLEDYVFRSKVTRASYERRAETVLHEMAHMWFGDLVTMKWWDDLWLNESFATFASV
                     LCQAEATEYKEAWTTFANAEKSWAYRQDQLPSTHPVAADIPDLAAVEVNFDGITYAKG
                     ASVLKQLVAYVGLEAFLAGLRDYFRDHAFDNATFGDLLGALEKSSGRDLSHWGRQWLK
                     TTGLNTLRADFDVDADGKFSRFAISQSGAQPGAGETRVHRLAVGIYDDDGSGKLVRVH
                     REELDIEGDSTEVPALQGVPRGKLIMVNDDDLTYCSLRLDPDSLETVLTRIADIAEPL
                     PRTLAWSAAWEMTREAELKARDFVALVMAGVHAETEVGVAQRLLLQAQTALRSYADPD
                     WACSDGWPAFADRLLELARGAEPGSDHQLAYVNALCTSVLALRHVAVLAALLDTDPAE
                     HGLPGLDIDTDLRWRIVTALCAAGDIDGESQATPFIDAEAERDPTAAGKRNAARAA"
     gene            complement(2251..2616)
                     /locus_tag="VGP27_14875"
     CDS             complement(2251..2616)
                     /locus_tag="VGP27_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7852615.1"
                     /translation="MIATGIALAAPASADPVDPNSDAFLSAINRAGIGYADNNPDLTA
                     QLGEQVCPMLVEPGKSFAQVATSVADNGISPGMASFFTGIAISMYCPQMMGSIGNGTF
                     LNTLQGLQGMPGVPQVPGF"
     gene            2790..3398
                     /locus_tag="VGP27_14880"
     CDS             2790..3398
                     /locus_tag="VGP27_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV7852616.1"
                     /translation="MPEKSAADFWFDPLCPWCWITSRWILEVEKVRDIDVKFHVMSLA
                     VLNEGRDLPEEYLEMMKKAWKPVRVAIAAEQAKGSEILAPLYTAMGTRIHNQDNKDFD
                     VVINESLAEVGLPAELAEAADTDKYDEALRKSHHEGMDPVGEDVGTPTIHVNGVAFFG
                     PVLSKIPRGEEAGKLWDASVTFASYPHFWELKRTRTEAPEFD"
     gene            3515..3994
                     /locus_tag="VGP27_14885"
     CDS             3515..3994
                     /locus_tag="VGP27_14885"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEV7852617.1"
                     /translation="MRVYLGSDHAGFELKNEIIEHLKKTGHEPIDCGAYTYDAEDDYP
                     AFCIATALKTVGDNESLGIVIGGSGNGEQIAANKVPGARCALAWSTETASLAREHNKA
                     QLIGIGARMHTVPEALEMVDAFLSTPWSGAERHQRRIDILKKYEETHVPPSVPGAPA"
     gene            3998..4792
                     /locus_tag="VGP27_14890"
     CDS             3998..4792
                     /locus_tag="VGP27_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV7852618.1"
                     /translation="MPEGHTLHRLARLHQRKFGRAPVIVSSPQGRFADGAAAVNGRVL
                     KKASAWGKHLFHHYEGGRIVHVHLGLYGKFSEVELPMPLPVGQVRMRMIGTDFGTDLR
                     GPTVCEVIEEPDVDDVIARLGPDPLRRDADGSLAWTRISKSRRPIGALLMDQTVIAGV
                     GNVYRNELLFRHRIDPYRFGVNIGEDEFDAMWTDLVELMKVGLRRGKIITVGREDDHG
                     LPSYLPGRPRTYVYRRAGEPCRICGTEVRTAVLEGRNLFWCPTDQT"
     gene            5006..5665
                     /locus_tag="VGP27_14895"
                     /pseudo
     CDS             5006..5665
                     /locus_tag="VGP27_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(5735..6556)
                     /locus_tag="VGP27_14900"
     CDS             complement(5735..6556)
                     /locus_tag="VGP27_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV7852619.1"
                     /translation="MNGVLLVLIILVVAAIGLAVWSASRNSASRNAASLADAKADARR
                     AIDRLGGQVINLTGTDDASKQALADASERFNAAGGQIEQATTAKQALLAKESANEGLY
                     YVRAARTAMGMDPGPELESLSGQKSAGVVTEDRRVNFEGRDIEASPNPTERTPNYYPG
                     GRVAGRPVPAGWYSEPWWKPALIAGAWGLGSVLLFDAMFSGMGGVGYDAQGFENGYGD
                     GYSDGFGAGQDAGYDGGGDMGGGDMGGSDGGGWGGDSWGGGDFGGGDFGGDFGGF"
     gene            complement(6601..>7721)
                     /locus_tag="VGP27_14905"
     CDS             complement(6601..>7721)
                     /locus_tag="VGP27_14905"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7852620.1"
                     /translation="ANFRDRNEVGAAIAVWVDGELAVNLWGGNADAAGQRRWRHDTLA
                     SIFSGSKALTSTCVHLLADRGELDLNDPVADYWPEFAQAGKQDITLATVMGHRSGVIG
                     PRTRLTPEETLDWDVVCDRIAKAEPWWQPGTAQGYHMVSFGFILGEIVRRVTGRTLGE
                     YLRKEIAEPLGIDVHIGLPAAEHHRCADMVNKPHIRDVLANGSAPADPTTLEEHPLAA
                     FSIAMGFIPDDELGSSDITAWRTAEFPATNAHVSARGLATFYNALAQGKLLSREHMEK
                     CRISQGGFDTDIVLGPRVADHGWGLGYMLNQRGMNGPNTKTFGHGGSGGSFGFVDLEH
                     RVGYAYVMNNFDATKCNADPRSVALSNEVYSALGVTRD"
BASE COUNT         1285 a         2376 c         2722 g         1338 t
ORIGIN      
        1 gcggcgcgag cggcgttgcg cttgcccgct gcggtgggat cgcgttcggc ttcggcatcg
       61 atgaaaggcg ttgcctggct ttcgccgtcg atgtcacccg ccgcgcacag cgcggtgacg
      121 atgcgccacc gcagatcggt gtcgatgtcg agcccgggca ggccgtgctc ggcgggatcg
      181 gtgtcgagca gcgcggccag cacggcgaca tgccgcagag cgagcaccga tgtgcacagc
      241 gcgttgacgt aggccagctg atggtccgag ccgggctcag cgccgcgggc cagttccagc
      301 agccggtcgg cgaacgccgg ccagccgtcc gagcaggccc agtccggatc ggcgtaggaa
      361 cgcagcgcgg tctgcgcctg aagcagcagc cgttgggcga caccgacctc ggtctcggcg
      421 tgcacacccg ccatgaccaa ggcgacgaag tcacgggcct tcagctcggc ctcgcgcgtc
      481 atctcccatg cagcggacca ggccagggtg cgggggagcg gttcggcgat gtcggcgatg
      541 cgcgtcagca cggtctccaa cgagtccggg tctagccgca gcgagcagta ggtcaggtca
      601 tcgtcattga ccatgatcag cttgccgcgc ggaacacctt ggagggcagg cacttcggtg
      661 gagtcgccct cgatgtcgag ctcttcgcgg tgtacccgga ccagcttgcc ggagccgtcg
      721 tcgtcgtaga tcccgacggc gagacggtgc acgcgggttt cgcccgcacc tggctgcgcg
      781 ccgctctggc tgatcgcgaa gcggctgaac ttgccgtcgg cgtcgacgtc gaagtcggcg
      841 cgcagcgtat tcagaccggt ggttttcagc cactgcctgc cccagtgcga caggtcgcgg
      901 cccgacgact tctccagcgc gccaagcaga tcgccgaagg tcgcgttgtc gaaggcgtgg
      961 tcgcggaagt agtcgcgcag cccggcaagg aacgcctcga gtccgacgta ggcgaccaac
     1021 tgcttgagca cgctggcgcc cttggcgtag gtgatgccgt cgaagttgac ctcgacggcg
     1081 gcaagatctg gaatgtcggc ggctacggga tgcgtcgacg gcaactggtc ctgccggtag
     1141 gcccacgact tctcggcatt ggcgaacgtc gtccacgcct ctttgtattc ggtggcttcg
     1201 gcctggcaca gcaccgatgc gaacgtcgcg aacgactcgt tgagccacag gtcgtcccac
     1261 cacttcatgg tcaccagatc gccgaaccac atgtgcgcca tctcgtgcag caccgtttcg
     1321 gcgcgacgct cgtaggacgc gcgggtgacc ttgctgcgga agacgtagtc ctccaaaaac
     1381 gttacggctc cggcgttttc cattgcgcct gcattgaact cgggcacaaa gagctggtcg
     1441 tacttgccga acgcgtacgg ctcaccgaaa ttgcggtggt agaagtcgaa gccctgcttg
     1501 gtctcggtga acagccggtc ggcgtccatg tactcggcca gcgacgtccg gcagtacagg
     1561 cccagcggga tctcgccgtg ctcgtcggtg taggcgtcgc gccagacggc gtacggcccg
     1621 gcgatcagcg cgacgaggta ggtgctcatc ttgggcgtcg tcgcgaaggt caaagtcttg
     1681 gcgccaccgg ctacctggac gtcagcgggg gcgccgttgg acaccacctc ccagtgcgcg
     1741 ggggcggtga ccttgatgtc aaatgtcgcc ttcaggtcgg gctggtcgaa acacgcgaac
     1801 atccttttcg catcggcggt ttcgaactgc gagtagaggt agacctcgtt gtcgaccggg
     1861 tcgacgaatc ggtgcaggcc ctcgccggtg ttcgagtaga ggcacaacgc ctcgacgacg
     1921 agggtgttgc gctcctgcag gccttgcagc gcgataccgg tggattcgtc gtacccggag
     1981 acgtcgaggt cgatgccgtt gagggtggcg cgctgcacgc tgtcggctgc gatgtcgatg
     2041 taggtgtcag cgccgggaag tgcgctgaac tccacggtcg taatggaccg gaacgtcttc
     2101 tcacctggcg cccctgcgcc atcggtcagg tcgaggtcta cgccgtagcg gtcgacggtg
     2161 acgagcgccg ctcgttcgat ggcttggtcg cgagtcagat tgggaagaac cacacctcca
     2221 acgtagtggt cccgctcgcg aggaggcaga ttagaagccc ggcacctgcg gaacgcccgg
     2281 cattccttga aggccttgca gggtgttcag gaacgtccca ttgccgatcg aacccatcat
     2341 ctgcgggcag tacatcgaga tcgcgatgcc ggtgaagaac gacgccatgc ccgggctgat
     2401 gccgttgtcg gcgacggacg tcgcgacctg ggcgaacgac ttacccggtt cgaccagcat
     2461 cgggcacacc tgctctccca gttgtgcggt gaggtcggga ttgttgtccg cgtagccgat
     2521 accggcacgg ttgattgcgg acaggaatgc gtcgctgttg gggtcgacag ggtcggcgga
     2581 tgcaggcgca gcgagcgcga tgccggttgc gatcatggtg gccgtgacgg ccgctgcgct
     2641 ggctcgattg attcgtgtca cttgtgtaac agtggttcac ctaggtcaca ttgacaacac
     2701 gcttcggtaa aggtttggaa cagcctcgcg cggcgcatgg ttgaggtgac tgagtttgtt
     2761 ccacgacacc cgaccgagag gattcgtcca tgcccgagaa gtccgccgcc gatttctggt
     2821 tcgacccgct gtgcccgtgg tgctggatca cgtcgcgatg gattctcgaa gtggagaagg
     2881 tgcgcgatat cgacgtgaaa tttcacgtga tgagcctcgc ggtgctgaac gagggccgtg
     2941 acctgcccga ggaatacctc gaaatgatga agaaggcatg gaagccggtg cgggtggcga
     3001 tcgccgccga gcaggccaag ggttcggaga tcctggcgcc gctgtacacc gcgatgggca
     3061 cacgcatcca caaccaggac aacaaggact tcgacgtcgt catcaacgag tcgctggcgg
     3121 aagtcgggct gccggctgag ttggcggagg ccgccgacac cgacaagtac gacgaggccc
     3181 tacgcaagag tcaccacgag ggcatggacc cagtcggcga ggacgtgggc acgccgacga
     3241 tccacgtcaa cggcgtcgcg ttcttcggtc cggtgctgtc gaagattccg cgcggcgagg
     3301 aagcgggcaa gctgtgggac gcgtcggtga ccttcgcgtc gtatccgcac ttctgggaac
     3361 tcaagcggac ccgcactgag gcgcccgagt tcgactgagc gttagtcggc gggtcccgcc
     3421 gagatcgacg aaatggcgag cccaactcgc acatcctcgc ccaaacgttg gtttgggcga
     3481 aagggcttca gcgggctctg ccagaatgac cgccatgcgc gtctacctgg gctctgacca
     3541 cgcgggcttc gaactcaaga acgagatcat cgagcacctc aagaagaccg ggcacgagcc
     3601 catcgactgc ggcgcctaca cctatgacgc cgaggacgac taccctgcgt tctgcatcgc
     3661 cacggcactg aagaccgtcg gcgacaacga gagcctcggc atcgtcatcg gcggctccgg
     3721 caacggcgag cagatcgccg ccaacaaggt gccgggcgct cgttgcgcgc tggcgtggag
     3781 caccgagaca gcctcgttgg cccgcgagca caacaaggcg cagctgatcg gcattggcgc
     3841 gcgcatgcac accgtgccgg aggcgctgga gatggtcgac gctttcctct cgaccccgtg
     3901 gtcgggagcc gagcggcacc agcgtcgtat cgacatcctg aagaagtacg aggagacgca
     3961 cgtgccgccg agcgtgccgg gcgcgccggc ctgacggatg cccgaaggcc acacgcttca
     4021 ccgactggct cggctccacc agcgaaagtt cggacgcgcg cccgtcatcg tctccagccc
     4081 gcagggccgc ttcgccgacg gggccgccgc cgtcaacggc cgggtgctga agaaggccag
     4141 cgcctggggc aagcatctgt tccaccacta tgagggcggc cgcatcgtgc acgtgcacct
     4201 cgggctctat ggcaagttca gcgaagtcga gctgccgatg ccgctgccgg tgggacaggt
     4261 gcggatgcgg atgatcggga cggacttcgg caccgacctg cgcgggccga cggtgtgcga
     4321 agtgatcgag gaacccgacg tcgatgatgt gattgcgcga ctcggcccgg acccgttgcg
     4381 gcgcgacgcc gacggttcgt tggcatggac gcgaattagt aagtctcgca ggccgatcgg
     4441 cgcactgctg atggatcaga cggtgatcgc gggagtcggc aacgtgtatc gcaacgagct
     4501 gctgtttcgt catcgcatcg acccgtaccg gttcggtgtg aacatcggcg aggacgaatt
     4561 cgacgcgatg tggaccgatc tcgtcgagct gatgaaggtg gggctgcggc gcggcaagat
     4621 catcacggtc ggccgcgagg acgaccacgg tctgccgtcg tacctgcccg ggcgtccgcg
     4681 gacctacgtc taccggcgcg ccggcgagcc gtgtcggatt tgcggcacgg aggtgcgcac
     4741 tgccgtgctc gaaggccgca atctgttctg gtgcccgacc gaccagacgt aggcggcccc
     4801 tgcctcatgc gccggccgtc actgcggcag cggtcgacgg ttcgacggcg cggccttgag
     4861 tctgcgtgta tggcggggaa ctgcggagaa gggtgcgccc tcaccgcaga gtcaacgcag
     4921 ggcctttcac tcagcgacaa tcggcgggtg gaactcattc tcgtcgtcgt cggcgccatc
     4981 gtcgtcaccg ccatggcgca tcggcggggc gctgggcacc cgacggcggg ccaagtcacc
     5041 ggacaagtgg cgttcgcgga tcgaccgtcg gccgttgagc tggcaggaga acatcgcggt
     5101 gtcgtggacg ggaatgcgcg gtgtagtgac tttggctgcg gcagcggcaa ttccggcgac
     5161 gacggtgtcc ggccagccgt tccccgaacg cgcggccatt caggcgatcg cgttcgtggt
     5221 gagcgtcggc acgttgttgg ttcagggctg gacgttgccg ctcctgattc ggcgtctgca
     5281 cctatcgagg ttcagcgacg attatgccgc cgaccgcgag gaggaactca aggccgaacg
     5341 cgtggtgcac gatgcggccg acgaggtgct ggcgcaattc cgggcgaacc cgccggcggg
     5401 aatggaccca cgcgtgctcg ccgagatccg cgccgtcgtc gcacggcatt cgcaggacgc
     5461 cgacgaaatg cccgatcccg atgcgcacac gaagcgatcc gaagtgttcg cggctttgta
     5521 tcgcgacgtc ttggcagcgc agcgggaggc attgatcgag gaacgcgatg agggtggcat
     5581 cgaggatgaa gcggtgcgcg gcatgctgga acggctggac ctgcaggagg ccggtgtgtc
     5641 agcgcgcctg gagagccggt tctaacttcg ccgagcagac gtcagcgccg gcaaaaccgc
     5701 agctggacgc gaactttcgc gttccggtgc gacgtcagaa gccgccgaag tctccaccga
     5761 aatcgccgcc gccgaagtcg ccaccgcccc agctgtcgcc accccagccg ccgccgtcgc
     5821 tgccacccat gtcgccgccg cccatgtcgc cgccgccgtc gtatccggcg tcctggccgg
     5881 caccgaatcc gtcggaataa ccgtcgccgt aaccgttttc aaagccttgg gcgtcatagc
     5941 cgaccccgcc catgccggag aacatcgcat cgaagagcag caccgagccc agcccccacg
     6001 cgcctgcgat gagtgccggt ttccaccacg gctcggagta ccagccggcc ggaaccgggc
     6061 ggcccgcgac gcgaccgccg gggtagtagt tcggggtgcg ctcggtcggg tttggtgagg
     6121 cttcgatgtc gcggccctcg aagttgacgc gccggtcctc ggtgacgacg cccgccgact
     6181 tctggccgga cagcgactcg agctcaggcc cggggtccat gcccatcgcg gtgcgggcgg
     6241 cgcgcacgta gtagagccct tcgttggcgc tttccttggc gagcagcgcc tgcttcgcgg
     6301 tggtggcctg ttcgatttgc ccgccggcgg cgttgaagcg ctccgaagcg tcggcgaggg
     6361 cctgcttgga cgcgtcgtcg gtgccggtca ggttgatgac ctggccgccg agccggtcga
     6421 tggctctgcg ggcgtcggcc ttggcgtcgg ccagggaggc ggcgtttcgg ctggctgaat
     6481 tgcgcgacgc gctccagaca gccaggccga tcgcagcgac gaccagaatg atgaggacga
     6541 gcaagacgcc gttcatgtga ccagcgtact cacaggaaat tgcggtttcg gggccctgaa
     6601 ctaatcgcgg gttacgccca gagcggagta gacctcgttg gagagtgcga cgctacgggg
     6661 gtcggcgttg catttggtgg cgtcgaagtt gttcatgacg taggcgtagc cgacgcggtg
     6721 ttcgagatcg acgaaaccga agctcccgcc cgagccgccg tggccgaagg tcttggtgtt
     6781 ggggccgttc atcccgcgtt ggttgagcat gtagccaaga ccccagccgt ggtcggcgac
     6841 gcgtggaccg aggacgatgt cggtgtcgaa gccgccctgc gagattcggc atttctccat
     6901 gtgttcgcgg gaaagcagtt tgccttgggc gagcgcgttg tagaaggtgg cgaggccgcg
     6961 ggccgacacg tgagcgttgg tggcggggaa ttctgctgtg cgccaagcgg tgatatcgct
     7021 ggagccgagt tcgtcgtcgg ggatgaagcc catcgcgatg gagaatgccg ccaacgggtg
     7081 ctcttcgagg gtggtcgggt ctgccggtgc gctgccgttg gcgaggacgt cgcggatgtg
     7141 gggcttgttg accatgtcgg cgcagcggtg atgctcggcg gcgggcaggc cgatgtggac
     7201 gtcgatgccg agcggctcgg cgatttcttt gcggaggtat tcgccgagag tgcggccggt
     7261 gacccggcgg acgatctcgc cgaggatgaa gccgaagctg accatgtggt agccctgcgc
     7321 ggtgccgggc tgccaccagg gttcggcctt ggcgatgcgg tcgcagacga cgtcccagtc
     7381 gagggtctcc tcgggtgtga ggcgggtgcg agggccgatg acgccggagc gatggcccat
     7441 cacggtagcg agggtgatgt cttgctttcc ggcttgggcg aattcgggcc agtagtcggc
     7501 gacggggtcg ttcaggtcga gttccccgcg atcggcgagg aggtgaacgc aggtgctggt
     7561 caatgctttc gagccggaga agatgctggc gagggtgtcg tgtcgccatc ttcgttggcc
     7621 cgctgcgtcg gcgttgccgc cccagaggtt gacggcgagt tcgccgtcga cccagacggc
     7681 gatggctgcg ccgacctcgt tgcgatcgcg gaagttggct t
//
LOCUS       DASYLX010000629         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2225155,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000629 DASYLX010000000
VERSION     DASYLX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225155"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VGP27_14910"
     CDS             <1..1282
                     /locus_tag="VGP27_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEV7852621.1"
                     /translation="LTGIVPVGLNPIRRGAALERDIRHADCQVVLADSASAAIVGDID
                     HIDVDSPQWAEEIAAHHAAPVTVRAADPADTFMLIYTSGTSGDPKAVICSHGKVAIAG
                     LMMTERFGLGADDVCYVSMPLFHSNAVLVGWAVSLACGGSMVLRRKFSASQFIPDVRH
                     YGVTYANYVGKPLSYVLATPERPDDADNPLRAVYGNEGAPADVERFARRFDTVVVDGF
                     GSTEGGVAIGRTPDTPPGALGPLPEGTDIVDVDTGEPCPPGVTGEIVNTTGPGRFEGY
                     YNDPGADAERMRGGVYHSGDLGYRDENGYAYFAGRLGDWMRVDGENLGAAPIERVLLR
                     HPDVIEVAVYGIPAPDVGDQVMAALLLADGAEFDSDAFREFLTEQPDLGPKQWPAFVR
                     IATELPRTESFKVIKRRLAAQGTECTDAVWPIRR"
     gene            1293..2018
                     /locus_tag="VGP27_14915"
     CDS             1293..2018
                     /locus_tag="VGP27_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7852622.1"
                     /translation="MGYRDFPPPSHLRDLVECAWVVDGPPEPVRVLPDGCMDLIRMSG
                     RIVVAGPDTTALLSPRNREAFAGLRFRPGALPRLLGVPAAALRDNRVALTDLGVKCSR
                     DRTLIELTTALASDEPVKETAPWSLHQLRRITDSLGNGAAVSDVARHTGWSSRTLQRQ
                     CAAVYGYGPAMLRRILRFRRAARLLDDGVSAADAAAAAGYADQPHLHREVRDLTGMPL
                     ASLRQGSGQKRSTHVPSGSVTVA"
     gene            complement(1958..>2242)
                     /locus_tag="VGP27_14920"
     CDS             complement(1958..>2242)
                     /locus_tag="VGP27_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7852623.1"
                     /translation="PHVEVDLPGGNKLAFDDEEIIAGMHPGWTPPTQQGRVALAFRVN
                     DPSDVDALFGRITGAGHAGALKPFDAPWGQRYATVTDPDGTWVDLFCPLP"
BASE COUNT          333 a          774 c          773 g          362 t
ORIGIN      
        1 cctgacgggg atcgtgccgg tcggtctcaa cccgatccgc cgcggcgctg cactggagcg
       61 cgatattcgg catgcggatt gccaggtcgt ccttgctgat tcggcgtcag ccgcgatcgt
      121 cggcgacatc gaccacatcg acgtcgactc gccgcaatgg gcggaagaga tcgccgcgca
      181 tcacgctgcg ccggtgacgg tgcgcgccgc tgaccccgcc gacaccttca tgctgatcta
      241 cacgtcgggt accagcggtg accccaaggc ggtgatctgc agccacggca aggtcgccat
      301 cgcgggcctg atgatgacgg agcgattcgg gctcggagcc gacgacgtct gttacgtgtc
      361 catgccgttg ttccactcga acgcggtgct ggtgggctgg gcggtgtcac tggcctgcgg
      421 aggctcgatg gtgttgcggc gcaagttctc tgcgtcgcag ttcattcccg acgtccggca
      481 ctatggggtg acctacgcca actacgtcgg caagccactg tcgtatgtgc tcgcgacgcc
      541 ggaacgtccc gacgatgccg acaacccgtt acgggcggtg tacgggaacg agggcgcgcc
      601 cgccgacgtc gagcggttcg cgcgccggtt cgacaccgtc gtcgtcgacg gattcggttc
      661 caccgaaggc ggagtcgcga tcggacgtac gccggacacc ccgccgggcg ccttgggccc
      721 gctgcccgag ggcaccgaca tcgtcgacgt cgatactggt gaaccatgtc cacccggcgt
      781 cacgggcgaa atcgtcaaca ccacgggacc gggacgcttc gagggctact acaacgatcc
      841 cggcgccgac gccgaacgca tgcgtggcgg cgtgtaccac agcggcgacc tcggctaccg
      901 cgacgagaac ggctacgcgt acttcgcagg gcggttgggc gactggatgc gcgtcgacgg
      961 cgagaacctg ggtgccgcgc cgatcgagcg ggtgctgctg cgccatcccg acgtcatcga
     1021 agtcgccgta tacgggatcc cggctcccga cgtcggcgac caggtcatgg cggcgctact
     1081 gcttgccgac ggggccgagt tcgactccga cgcattccgc gagttcctga ccgagcaacc
     1141 ggacctgggc cccaagcagt ggcccgcatt cgtgcgcatc gccaccgagc tgccgcggac
     1201 cgagtcattc aaggtcatca aacgccggct cgcggcgcag ggcaccgagt gcacggacgc
     1261 ggtgtggccg attcggcggt aacgtcgatg aggtgggtta ccgcgacttc ccaccccctt
     1321 cgcacctgcg cgacctcgtc gagtgcgcgt gggtggtgga cggcccgcct gagccggtcc
     1381 gggttctgcc cgatggctgc atggacctca tcagaatgtc gggccgcatc gtcgtcgccg
     1441 gtccggacac cacggcactt ctcagcccgc gtaatcggga agcgttcgcg ggcttgcggt
     1501 ttcggcccgg ggccctgccc cggctgctcg gcgttccggc ggcggcactg cgcgacaacc
     1561 gcgttgcact gacggatctt ggcgtcaagt gttcgcggga ccgtacgttg atcgagctga
     1621 cgaccgccct tgcgtccgac gagccggtga aggagacggc gccgtggtcg cttcaccagc
     1681 tacgccgcat caccgacagc ctcggtaacg gtgccgcagt atcagacgtg gcccgccaca
     1741 ccggttggtc gagccgcacg ctgcagcgcc aatgcgccgc cgtctacggc tacgggcccg
     1801 cgatgctgcg gcgcatcctg cggttccgcc gcgccgcccg actgctggac gacggcgtgt
     1861 ccgcggccga cgcggcggcc gcggccggtt acgccgacca gccgcacctg caccgcgagg
     1921 tccgtgattt gaccggcatg ccgctggcgt cgctgcgtca gggcagcggg cagaagaggt
     1981 cgacccacgt gccgtctgga tcggtcacgg tcgcatagcg ctggccccac ggggcgtcga
     2041 acggtttcag ggcgccggca tgccctgcgc cggtgatccg cccgaaaagc gcgtcgacgt
     2101 ccgacgggtc gttcaccctg aacgccagcg ccacccggcc ctgttgcgtc ggcggggtcc
     2161 agccggggtg catgccggcg atgatctcct cgtcgtcgaa ggccagcttg ttgccgccgg
     2221 gcaggtcgac ttcgacatgc gg
//
LOCUS       DASYLX010000630         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2554757,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000630 DASYLX010000000
VERSION     DASYLX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554757"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VGP27_14925"
     CDS             <1..919
                     /locus_tag="VGP27_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV7852624.1"
                     /translation="FVGRGDGFGLTIVDRNENDPRLKYYRFQTSAIGWNPGVNVLLPD
                     DYATSGRRYPVLYMFHGGNDDFRQFDFLGIRDLTAGKPIIVVMPDGGHAGWYSNPVSS
                     FVGPRNWETFHIAQLLPWIEANFRTFAEYDGRAVSGFSMGGFGALKYAAKYYGHFASV
                     SAHSGPASLRRDFGLVVHWANITSAVLDLGGGTVYGAPNWDQARVSADNPVERIESYR
                     NKRIFLVAGTSPDPLNWFDSVNETQVLAGQREFRERLSGAGIPHESHEVPGGHVFRPD
                     MFVLDLDGIIARLRHASSAASTGASDGDE"
     gene            906..2162
                     /locus_tag="VGP27_14930"
     CDS             906..2162
                     /locus_tag="VGP27_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016891475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV7852625.1"
                     /translation="MATNRYMPLKAGTYHLSSPFGPRDRQMHWGQDFAADDGTHIYAA
                     QAGSVQYIGAAQGFGQWIVIDHPAPAGGGTTVYGHMWDAFATGLKRGDSVAPGQHIAY
                     VGSNGQSSGPHLHFEVHPTVWRAGSQIDPMPWLADALDPETRLAVSAVPVTDTLYADV
                     SEWQRPVDDSYPYRVIAIRSNDGTYRDKKWASNYAWCKRRCDENALVFFIVYFVWRQN
                     WQQTVDTLKALVGAPHPRMAVMIDVESWQGQIIGDQSDGLNKAFQTIADWLGDRRRVI
                     AYGNRGDLNSLWPDKPDGLRIVLAAYGSNPGYPGKIAHQYTNGEGYGGGLPEGCPPFG
                     KCCMDSADGLTPEQYAAGCGIGAAVVGERTGHGVFTDDDSVVLAAVEQPQQKTVTDRR
                     AAAEATPTKTARSQANPAERRTEPRP"
BASE COUNT          425 a          729 c          692 g          375 t
ORIGIN      
        1 attcgtcggg cgcggcgacg ggttcgggct gacgatcgtc gatcgcaatg agaatgaccc
       61 tcgcttgaag tactaccggt ttcagacctc ggcgatcggc tggaaccccg gcgtcaatgt
      121 cctgcttccc gacgactacg ccactagcgg acgcagatat cccgtcctct acatgttcca
      181 cggcggcaac gatgacttcc gccagttcga ctttctgggt atccgcgatc tgaccgccgg
      241 aaagccgatc atcgtcgtga tgcccgacgg cggacacgcg ggctggtact ccaacccggt
      301 cagctcgttc gtcggcccgc ggaactggga gactttccac atcgcccagc tgctcccgtg
      361 gatcgaagcg aacttccgaa cattcgctga gtacgacggc cgcgcggtct ccgggttttc
      421 catgggtggc ttcggtgcgc tcaagtacgc agccaagtac tacggccact tcgcctcggt
      481 gagcgcccac tccggcccgg cgagcctgcg ccgcgacttc ggcctggtcg tgcactgggc
      541 aaacatcacg tcggcggtcc tggacctggg cggcggcacg gtgtacggcg cgcccaactg
      601 ggaccaggcc agggtcagcg ccgacaaccc ggtcgagcgg atcgaaagct accgaaacaa
      661 gcggatcttc ctggtcgccg gcaccagtcc cgatccgctc aactggttcg acagcgtgaa
      721 cgaaacacag gtgctcgccg gccaaaggga gttccgcgaa cgcctcagcg gggccggcat
      781 cccgcatgaa tcgcatgagg tccccggcgg ccacgtcttc cggcccgaca tgttcgtcct
      841 cgacctcgac ggcatcatcg cccggctgcg ccacgccagc agtgctgcgt ctacgggtgc
      901 ttccgatggc gacgaatagg tacatgccgc tgaaggcggg aacgtatcac ctgtcctcac
      961 catttggtcc gcgcgaccgt cagatgcatt gggggcaaga tttcgcggcc gacgacggca
     1021 cgcacatcta cgcggcgcaa gccggatccg tccaatacat cggagcagca caaggtttcg
     1081 gccagtggat cgtgatcgat caccccgctc cagcaggagg cggcaccacc gtctacggcc
     1141 acatgtggga cgcctttgcc accggcctga agcgaggcga cagcgtggcc cccggtcagc
     1201 acattgccta tgtcggaagc aacggccaat cctccggccc acatctgcac ttcgaagtgc
     1261 accccacagt ctggcgggcg ggctctcaaa tcgatccgat gccgtggctg gccgacgcgc
     1321 tggatccgga gacgcggctc gcggtgtcgg cggtcccggt caccgacaca ctctatgccg
     1381 acgtatcgga atggcaacgc ccggtggatg attcctaccc ctaccgagtg atcgccatcc
     1441 ggtccaacga tggcacgtat cgggacaaga aatgggcgtc gaactatgcc tggtgcaaga
     1501 gacgctgcga cgagaatgcg ctggtctttt tcatcgtcta cttcgtctgg cggcagaact
     1561 ggcaacagac cgttgacacg ctcaaggccc ttgtcggagc gccacatcca cgcatggccg
     1621 tgatgatcga tgtggagagc tggcagggac aaatcatcgg cgaccaatcc gacgggttga
     1681 acaaggcctt ccagacgatc gcggactggc tcggcgatcg gcgacgagtc atcgcctacg
     1741 gcaacagggg cgatctgaac agtctgtggc ccgacaagcc ggatggactt cgtatcgttc
     1801 tcgccgccta cggatcaaat cccggctacc ccggcaaaat tgcccaccag tacaccaacg
     1861 gggagggtta cggcggcggc ctgccggagg ggtgtccgcc attcggcaag tgctgcatgg
     1921 attccgccga cgggctcacc cccgaacagt acgcagctgg ctgcggtatc ggcgccgctg
     1981 tagtgggcga gcgtacgggc cacggcgtgt tcaccgacga cgattccgtc gtgcttgccg
     2041 ccgtggagca gcctcagcag aagaccgtga ccgaccggcg agctgctgcg gaagcgacgc
     2101 cgacgaaaac agcccgctcc caagccaatc cagcggaaag gaggacggag ccgcggccgt
     2161 agcggatcaa gaatcgtcag gatgcttctt ggcgtgcgca acccaatcca ggtggttcgg
     2221 g
//
LOCUS       DASYLX010000631         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1788001,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000631 DASYLX010000000
VERSION     DASYLX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788001"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VGP27_14935"
     CDS             <1..1251
                     /locus_tag="VGP27_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEV7852626.1"
                     /translation="ASTGVRTELRRADGLGFDIAIEDAKISLAQIHSRVKTLARSQSA
                     DIGSQLLNAGVNVVAGRAELVDSVPGMAHHRVKVTTSDGKVGVLKADVVLIATGASPR
                     VLPQAVPDGQRILNWRQLYDLSELPEHMVIVGSGVTGAEFCNAYTELGVKVTVVASRD
                     QILPHEDSDAAAVLEDAFNERGVSLIKNARAESVTRHDDGVVVKIADGRTVEGSHALM
                     TVGSVPNTSGLGLEKVGIELGRGNYLPVDRVSRTSASGIYAAGDCTGLLPLASVAAMQ
                     GRIAMYHALGEGVSPIRLRTVAAAVFTRPEIAAVGVPQTKIDDGTVPARTLMLQLTTN
                     ARAKMSLLRRGFVKIFCRPATGVVIGGVVVAPIASELILPIALAVQNNLTVTDLAQTL
                     SVYPSLSGSIVEAARRLMAHDDLD"
     gene            1329..>2272
                     /locus_tag="VGP27_14940"
     CDS             1329..>2272
                     /locus_tag="VGP27_14940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEV7852627.1"
                     /translation="MSDPIAGPGSGQTLLSPDQRASAWERLAGEQFDVVVIGGGVVGA
                     GAALDAATRGLKVALVEARDFASGTSSRSSKMFHGGLRYLEQLEFGLVREALHERELS
                     LTTLAPHLVKPLPFLFPLTHRWWERPYVAAGIFLYDQLGGAKSLPPQKHLLKGGALRL
                     APGLKRSSLIGGIRYYDTVVDDARHTMTVARTAAHYGAVVRTSTQVVALLREGDRVTG
                     VKVRDSENGAVAEVHGHVVVNATGVWTDEIQALSKQRGRFRVRASKGVHIVVPRDRIV
                     SEVAIILRTEKSVLFVIPWGTHWIIGTTDTDWNLDLAHP"
BASE COUNT          366 a          753 c          778 g          375 t
ORIGIN      
        1 gcgtcgacgg gagtgcgcac cgagttgcgc cgcgccgacg gcctcggctt cgacatcgcg
       61 atcgaggacg cgaaaatctc acttgcgcag atccacagcc gggtgaagac gctggccagg
      121 tcgcagtccg cggacatcgg cagccagttg ctgaacgcgg gcgtcaacgt cgtcgccggt
      181 cgtgccgaac tggtcgacag cgtgccgggc atggcgcatc accgtgtgaa ggtcaccaca
      241 tccgacggca aggtcggcgt gttgaaggcc gacgtcgtgc tgatcgccac cggagccagc
      301 ccgcgtgtgc tgccccaggc cgtgcccgac ggccagcgca tcctgaattg gcggcaactc
      361 tatgacctca gcgagttgcc cgagcatatg gtgatcgtcg gctccggcgt caccggcgcg
      421 gagttctgca acgcctacac cgaactgggc gtcaaggtca ccgtggtggc cagccgcgac
      481 cagatcctgc cgcacgagga ctccgatgct gccgccgtgc tcgaggacgc gttcaacgaa
      541 cgcggcgtgt cgctgatcaa gaacgcccga gccgaatcgg tgacccgcca cgatgacggt
      601 gtggtggtga agatcgccga cggccgcacg gtcgaaggca gccacgcgtt gatgacggtc
      661 ggctcggtgc ccaacacgtc cgggctcggg ctggagaagg tcggtatcga actcggccgt
      721 ggcaattacc ttcccgtgga ccgggtttcg cgaacctcgg catccggcat ctatgcggcg
      781 ggcgactgca ccggtctgct tccgctggcg tccgtcgccg ccatgcaggg ccgcatcgcg
      841 atgtatcacg ccctcggtga gggggtctcc ccgatccgcc tgcgcaccgt cgccgccgcc
      901 gtgttcaccc ggcccgagat cgcggcagtc ggcgttccgc agaccaagat cgacgacggc
      961 acggtgcccg cgcgaacgtt gatgttgcag ctgacgacga acgccagggc gaagatgtcg
     1021 ctgctgcgac gcggcttcgt caagatcttc tgcaggcccg ccaccggggt ggtgatcggc
     1081 ggcgtcgtgg tcgccccgat cgcgtcggag ttgatcctgc cgatcgcgct cgcggtgcag
     1141 aacaacctca ccgtgaccga cctcgcccag acgctctcgg tgtacccgtc gctgtcgggg
     1201 tcgatcgtcg aagcggcccg gaggctgatg gcgcatgacg acctcgactg atgccgcgca
     1261 ggcgaggagc ggaatacacc accaagtagc ctgttcttgt actgacaagt aacttaggag
     1321 cctctcgcgt gagcgacccg atcgccggtc cgggcagcgg gcaaaccctt ttgagccctg
     1381 accagcgcgc atccgcgtgg gaacgactgg ccggcgagca gttcgacgtc gtcgtcatcg
     1441 gtggcggtgt ggtcggcgcg ggtgccgcgc tcgacgccgc gacccgcggg ctgaaggtcg
     1501 cactcgtcga agcgcgcgac ttcgcgtcgg gcacgtcgag tcgcagcagc aagatgttcc
     1561 acggcgggct gcgctacctg gaacagctgg agttcggact cgtgcgtgag gccctgcacg
     1621 agcgcgagct gtccttgacg acgttggcgc cgcatctggt caagccgctg ccgttcctgt
     1681 tcccgctgac gcaccggtgg tgggagcggc cgtatgttgc cgcgggcatc ttcctgtacg
     1741 accaacttgg cggcgcgaaa tcccttcccc cgcagaagca tctgctgaag ggaggcgcgc
     1801 tgcggctggc tccgggtctg aaacgcagct cgctgatcgg cggtatccgc tattacgaca
     1861 ccgtcgtcga cgacgcgcgg cacaccatga cggtggcgcg caccgcggcg cactacggcg
     1921 cggtggtgcg gacgtcgacg caggtcgttg ccctgttgcg cgaaggcgac cgggtcaccg
     1981 gcgtcaaggt ccgcgactcc gagaacggcg ccgtcgctga ggtgcacggc catgtggtcg
     2041 tcaacgccac gggtgtgtgg accgacgaga tccaggcgct atccaagcag cgcggccggt
     2101 ttcgggtccg ggcgtcgaag ggtgtgcaca tagtcgtgcc gcgcgaccgc atcgtcagcg
     2161 aggtggcgat cattctgcgc accgagaagt ccgtgctgtt cgtcattccg tggggcacgc
     2221 actggatcat cggcaccacc gacaccgact ggaacctcga ccttgctcac cc
//
LOCUS       DASYLX010000632         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2774001,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000632 DASYLX010000000
VERSION     DASYLX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774001"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGP27_14945"
     CDS             <1..819
                     /locus_tag="VGP27_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7852628.1"
                     /translation="YLAMSGDGLPGPTDSWVTLGALARETTSIRLGTMVTSATFRYPG
                     PLAVSVAQIDEMSGGRVELGLGAGWFEAEHKAYAIPFPPLGERFERLREQLDIITGLW
                     TTPNGQTFDYTGKHYSVTDSPALPKPVQRPHPPIIIGGAGAKRTPALAAAFAAEFNAP
                     FMPLETVRTTYERVRAALAEAGRSPDSLTYSAAFVVCAGRDDAQVATRAAAIGRDVDE
                     LRDNSPLVGTPNEIVDKLGPFAEAGVQRVYLQLLDMSDLDHLDLFASEVLPRLG"
     gene            861..1349
                     /locus_tag="VGP27_14950"
     CDS             861..1349
                     /locus_tag="VGP27_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852629.1"
                     /translation="MASPDLDDEFDKDEFVDEFGGDEPAEPWHNSTRAVVGASAAGVA
                     AIGILVAAVMYVTGQDGPNAPTDFVDRSFTATASDTPATTTTATITSTAPVSTTEINA
                     PLTPTTSGLPNTSGSSSSPSSSTESTPRPRQRENDGNPGGPTSRRPRFNVTRTLQPQQ
                     PG"
     gene            1377..1898
                     /locus_tag="VGP27_14955"
     CDS             1377..1898
                     /locus_tag="VGP27_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852630.1"
                     /translation="MARYGPPDDPNYYSDSEPTAYAANYGGGDYGGYEQPDQPPEPPV
                     PWYRRPAALVGFGALVAILIALIVWLLVSLMSDSSDSTSTTTSTTTTTSAPSGSAPAV
                     APTQTVTETTSPPSTETTAPTGTTTSTEPSVSTSISTSVSTSTSTVTVSPPSSNPQVP
                     QVPQIPQIPIPGG"
     gene            complement(1959..>2708)
                     /locus_tag="VGP27_14960"
     CDS             complement(1959..>2708)
                     /locus_tag="VGP27_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoniazid-inducible protein iniC"
                     /protein_id="HEV7852631.1"
                     /translation="VVPVSGLLALTARTLRQSEYVALEKLAGVDAAELAKAMLSADRF
                     VREDSRLPVDAATRAALLDRFGMFGLRISTAVLRAGVTDSVGLADELLERSGLVALRD
                     VIDQQFAQRSDLLKAHTALLSLRQFVQNNPIYASPYIIADIDPLLADTHAFEELRLLS
                     QLRSRSTTLNDDEMVSLRRIIGGSGTDVASRLGLQPGDPYDGPRAAFAAAQRWRRRAD
                     HPLNDPFTTRACRAAVRSAEAMVAEFAARGR"
BASE COUNT          443 a          996 c          890 g          379 t
ORIGIN      
        1 tacctggcca tgagcggcga cgggttgccc gggcccaccg actcgtgggt cacgctggga
       61 gccctcgccc gcgagacgac ctcgatcagg ctcggcacca tggtcacctc ggcgaccttc
      121 cgctatcccg gcccgctggc cgtctcggtc gcccagatcg acgagatgag cggcggccgc
      181 gtcgaattgg gcctcggcgc cggctggttc gaggccgaac acaaggccta cgcgatcccg
      241 ttcccgccgc tcggcgagcg cttcgagcgg ctgcgcgaac aactggacat cattaccggg
      301 ctgtggacca cacctaacgg ccagacgttc gactacaccg gcaagcacta ctcggtcacc
      361 gattcgccgg cgctgcccaa gcccgtccag cggccccacc cgccgatcat catcggcggt
      421 gcgggcgcca agcggacgcc cgccctcgcg gcggcgttcg ccgccgagtt caacgccccg
      481 ttcatgccgc tggagactgt caggaccacc tacgagcggg tgcgcgcggc actcgccgag
      541 gccgggcggt cgccggactc gctgacgtac tcggcggcgt tcgtggtgtg tgcaggccgc
      601 gacgacgccc aggtggccac gcgcgcggcg gcgatcggcc gcgacgtgga cgagcttcgc
      661 gacaactctc cgctggtggg cacgcccaac gagatcgtcg acaagctcgg accattcgcc
      721 gaagccggcg tgcagcgggt ctacctgcag cttctggaca tgtccgacct cgatcacctc
      781 gacctgttcg cctccgaggt gctaccccgg ctcggctgag gtaggcgtta tcgcaggtcg
      841 tgggattaac atcggtggcc gtggcgagtc ccgacctcga tgacgaattc gacaaggatg
      901 aattcgtcga tgagttcggc ggcgacgagc cggccgagcc ctggcacaac tcgacgcggg
      961 cagtggtcgg cgcgagcgcc gcgggcgtcg ccgccatcgg cattctcgtc gccgcggtca
     1021 tgtacgtcac cgggcaggac gggcccaatg cgcctaccga cttcgtcgat cgctcgttta
     1081 cggcgaccgc ctccgacacg cctgccacga cgacgaccgc caccatcacc agcaccgcgc
     1141 cggtgtccac caccgagatc aacgccccgc tcacgccgac gacctccggt ttgccgaaca
     1201 ccagcggcag cagcagcagt ccgagcagca gcaccgagtc cacgccgcgc ccgcgccagc
     1261 gcgagaacga cggaaatccc ggcggcccca cgtcgcggcg gccgcgcttc aacgtgacac
     1321 gcacattgca gccccagcag ccgggctgat cagggccagc gcctaatatc gggcccgtgg
     1381 cgcgatacgg acctcccgac gaccccaact attactcgga cagcgagccg accgcgtacg
     1441 cggccaacta cggcggcggc gattacggcg gctacgagca accggaccag ccgcccgaac
     1501 cgccggtgcc ctggtatcgc agacccgcgg cgctcgtcgg tttcggcgcg ctggtcgcga
     1561 ttctcatcgc cctcatcgtg tggttgttgg tgagcctgat gtcggattcg tcggacagca
     1621 ccagtactac gacctccacc acgacgacga cgtccgcgcc gtcgggatcg gcccccgcgg
     1681 tcgcaccgac acagaccgtc accgagacga cctcaccgcc gtcgaccgag acgacggcac
     1741 cgaccgggac gacgacatcg actgagccga gcgtcagcac gtcgatcagt acgtcggtca
     1801 gcacgagcac cagcaccgtc acggtgtcgc cgccgtcgag caacccgcag gttcctcagg
     1861 tgccccagat cccgcagatt ccgatccccg ggggatagcc gagggctcag cggctcacgc
     1921 agtctcggcc gctcagcggc tcacgcagtc tcggccgctc agcggccgcg cgcggcgaat
     1981 tcggccacca tggcctcggc gctgcgcacc gccgcgcggc atgcgcgggt ggtgaacgga
     2041 tcgttgagcg gatggtccgc ccggcgccgc caccgctggg cggcggcgaa cgcggcccgc
     2101 ggcccgtcgt acggatcacc gggttgcagg ccgagcctgc tggccacgtc ggtgccggag
     2161 ccaccgatga tgcggcgcag cgataccatc tcgtcgtcgt tcaatgtcgt tgagcgcgaa
     2221 cgcaattggc tcaaaagccg cagctcctcg aaggcgtggg tgtcggcgag caacgggtcg
     2281 atgtcggcga tgatgtaggg gctggcgtag atcgggttgt tctgcacgaa ctggcgcagc
     2341 gacagcagcg ccgtatgggc cttcagcagg tccgagcgct gggcgaactg ctggtcgatg
     2401 acgtcgcgca gcgcgacaag accgcttcgc tcgagcagct cgtcggcaag accgaccgag
     2461 tcggtcaccc ctgcccgcaa gacggccgtg gagatccgca ggccgaacat gccgaaccgg
     2521 tcgagcagcg ccgcgcgggt cgccgcgtcg accggcagcc ggctgtcctc ccgcacgaac
     2581 cggtccgccg acagcatcgc cttggccagt tcggcggcgt ccaccccggc gagcttctcc
     2641 agcgccacgt actcgctctg acgcagggtg cgtgcggtca gcgccagcag ccccgacacc
     2701 gggaccac
//
LOCUS       DASYLX010000633         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2554966,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000633 DASYLX010000000
VERSION     DASYLX010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554966"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGP27_14965"
     CDS             <1..399
                     /locus_tag="VGP27_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7852632.1"
                     /translation="PINAILLVSAISVVLVLFFVGQIGLISSLVNFGALFAFCLLHIS
                     VVWYYIGHKKSKNYLLHLVVPTLGFLIIGYVLFNADPLAKIGGIVWLAIGAVVFIVNR
                     VTGRGVTDFSEEKQVEGEGAAAESTKSVQE"
     gene            887..1171
                     /locus_tag="VGP27_14970"
     CDS             887..1171
                     /locus_tag="VGP27_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEV7852633.1"
                     /translation="MRYRVELELLLEFVDKLQAFERHAAAIAARVDEQVTDLHETWDG
                     AGAAAHNTLHQEWMSAAQQMREALAQLREAADRAHRNYSEAAQLNVAMLT"
     gene            1817..2152
                     /locus_tag="VGP27_14975"
     CDS             1817..2152
                     /locus_tag="VGP27_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852634.1"
                     /translation="MQDYKAALDDLRRQLEDILTDLAVELAVTAVISIFAACVSFGVG
                     AAAGTAKAAHTITKFATTTRSAVGAWKISKRISEGVQKAHDIAGTRKRLEGSRISGEK
                     RSRRSRRPG"
     gene            2188..2478
                     /locus_tag="VGP27_14980"
     CDS             2188..2478
                     /locus_tag="VGP27_14980"
                     /inference="COORDINATES: protein motif:HMM:NF022869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin D domain-containing protein"
                     /protein_id="HEV7852635.1"
                     /translation="MPGQIEKKFDKHAADFGVIEPRGKSGFKAFENAVRQQVDDPATL
                     HISGIYKGDPAIINYNPDSGLAVIQKPNGEFVSGWRLDLDQTRNVLDRGSLR"
     gene            complement(2862..>4064)
                     /locus_tag="VGP27_14985"
     CDS             complement(2862..>4064)
                     /locus_tag="VGP27_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7852636.1"
                     /translation="LTAAQARRVAVAAQGFHEPAPRGPVTRAHLKRLVSRIQVLQLDS
                     VSVAVRAHYAPVFSRLGPYDRDVVDRAAWSHSARSPRLLVEYWAHEAALMAVDDWPLL
                     RWRMREYTGGRWGKEIVKKNARLADDVVAAVAELGPSTAGQIESHLGAGQRGKKGPWW
                     DRSDTKWVTEALFAAGVLTTATRVGFARHYDLVENVLPPEVLAREVDDDEAVRELVLR
                     AAGALGVGTEADIRDYFRLGAAQVKPAIAKLVADGELEAVEVDGWKAPAYLRAGQIVP
                     RVERGTALLCPFDPLIFFRPRVERLFLFRYRIEIYTPAPKREFGYYVWPFLLDGQLVG
                     RVDLKAERVSDALNVVGAFTEEGQDAARVASALAGELRSMAGWLGLSDVTVGERGDLV
                     GALRAALA"
BASE COUNT          807 a         1320 c         1273 g          664 t
ORIGIN      
        1 ccgatcaacg cgatcctcct ggtgagcgcg atcagtgtcg tgctcgtgct gttcttcgtc
       61 ggccagatcg ggctgatctc gtcgctggtg aacttcggcg cgctgttcgc cttctgcctg
      121 ctgcacatct ccgtcgtctg gtactacatc gggcacaaga aatcgaagaa ctacctgctg
      181 cacctggtcg ttccgacgct cggtttcctg atcatcggct atgtgctgtt caacgccgac
      241 ccgctggcca agatcggcgg cattgtgtgg ctggcgatcg gcgcggtggt gttcatcgtc
      301 aacagggtca ccggccgggg cgtgacggac ttcagcgagg aaaagcaggt cgagggcgag
      361 ggcgccgccg cagagtcgac aaaatcagtg caggagtgac gcttcgccgc ccataccctt
      421 gataggtggc tcgatgggtt cgcgactgtg cggattctgt cgagaatcgc ttcagaggca
      481 tccgaatccg cacactcacc aggagccatg tcaatgcaca aggtcggcaa attagtgcag
      541 gagtgaccct cagccgccca tacgctttat atgtggccag ccagatacgg gtcgacgcac
      601 cccgagcgct ccaaacttcg cacgccgtag gcggtgacgt cgaggaccta tctgcggagc
      661 tcgcgagact gtcgcgggaa tggaacagcg tgctgtcggg atggtcaggt gctgcggcgt
      721 cttcatatgc cgccctgtcg gaagagtggc acgacggggc ggccaagctc gttgagaccc
      781 tggcccgttc gtctcatctg ctcgagcggg ccgccgtggc ttacgaccag cgagagacta
      841 cttccgcgca atccgtgcga agtgtttcca cccgaatcgt ttcgtgatgc ggtatcgagt
      901 cgaactcgag ctcctgctgg agttcgtcga caaacttcaa gccttcgagc gccacgccgc
      961 agcgatcgct gctcgcgtcg acgaacaagt caccgacttg cacgagacgt gggacggtgc
     1021 gggcgccgcc gcgcacaaca cactgcacca ggagtggatg agcgccgcgc aacagatgcg
     1081 agaggcgttg gcgcagttgc gagaagccgc ggacagagcg caccgcaact acagcgaggc
     1141 cgcccagctg aacgtagcga tgctgacgtg acgcaggacg tcgacaccga cacctactac
     1201 gcggtcggcc ggcggatgtt cgagttggcc ggtgatgttt acgacgcgtt cgcggtgaat
     1261 gtgggcatcc tgagcgcgac aggcgcgatg gctggcaccg acgatgcggg caccgcatgg
     1321 gcggcgtctt acgacggtcg cgcgaaagaa gttctggctt cggtcaacga tctgaccaag
     1381 gcgctggaga actacggcgg cgtggtgatc caggcgggct ataaccacgc ggtcgccgag
     1441 tacaacgcca caccgggaaa ccggcgtccg tcgcctcagc gcccgcctga gccggcgagt
     1501 gtcgcaggag tgctcacggc gccgccatcg gcgggtggcc caggcagggg tctgttggat
     1561 gacgcgctcg ggctggtgga gcaggtcggc gtgccggtgc ccgaggcgac accgtcaagg
     1621 tcgacaaggc cgcgcaggcc tgggaccgac tggcgacggt gtaccagacg aaaacggtcg
     1681 tcgaagcact ggaagtcaac gcccgagcct tcagtgacac acattcaccg gaggtcgacg
     1741 acatcgttcg ggacttgcac gagctgcggg atgcaacatc ggctgtcctg gccgcctgca
     1801 gtgaactggc gcagtcgtgc aggattacaa ggcagcactc gacgatctgc gccgacagct
     1861 ggaagacatc ctgaccgacc tggcggtcga gttggcggtg acggcggtga tctcgatttt
     1921 cgccgcatgc gtcagcttcg gcgtgggtgc ggcagcgggg accgccaagg ccgcgcacac
     1981 gatcaccaag ttcgccacga ccaccaggtc cgcggtgggt gcgtggaaaa tctcgaagcg
     2041 gatcagcgag ggcgtccaga aggcgcacga catcgcgggg acacgtaagc gcctcgaagg
     2101 atcaagaatc tcgggcgaaa agcgaagccg gaggagccga aggccgggct aggcgcgcag
     2161 acaacttcgg gcactccact gacgaccttg ccaggccaaa tcgagaagaa atttgacaaa
     2221 catgcggccg atttcggggt gatcgagcca cgcggcaaga gtgggttcaa ggcgttcgag
     2281 aacgcggtca gacaacaggt cgatgatcca gctacccttc acatcagcgg catctacaaa
     2341 ggagatccag cgattatcaa ctacaacccg gactccggtc tcgccgtaat ccagaagcca
     2401 aacggcgagt ttgtcagtgg ctggcgactg gacttggacc agacgagaaa cgtgttggat
     2461 aggggctcac ttcgttgagc aaactgccgg acattggttt gtaccttcgc cttctagaag
     2521 atttcatcgc tgaacgtata agcgcgcccg aattcgagaa gtcatacctt tcagctgtga
     2581 aatcagaacg acaaattctt ggagacccgg tgtttcccgt tcttcagaga ctgtttgaag
     2641 atgccgacga ctacgtggaa taccccgagc tgagaacaga gccagaggac ctcgacgatg
     2701 accaactccg gaagtgtgcc gttcgtgcta gaagcgaact gcgagaactc gggttcgatt
     2761 agcgccgttg aggttcgagt gcgctcacag gactggcccg actgtgcgct ttgggagcaa
     2821 aaaggagtgc gaggcatcca aatccgcaca tccgcaacga cttacgccag cgcagcacgc
     2881 agcgcgccaa ccaggtcacc gcgctcgccg accgtgacgt cgctcaaccc caaccagcca
     2941 gccatcgacc gcagctcccc cgccaatgcg ctggccacac gcgccgcgtc ctggccctct
     3001 tcggtgaacg caccgacgac attcaacgca tccgacaccc gctccgcctt cagatcgaca
     3061 cggccgacca gctggccgtc gagcagaaat ggccatacgt aataaccgaa ttcgcgcttc
     3121 ggagcgggag tatagatctc tatgcggtac ctaaacagaa acaaccgctc gacgcgcggc
     3181 ctgaagaaga tcagcggatc gaacggacac agcagcgccg tgccccgctc gacgcgcggc
     3241 acgatctggc cggcacgcag gtacgccggc gccttccagc cgtcgacctc aacggcttcg
     3301 agctcgccgt ccgcgaccag cttggcgatc gccggcttga cctgcgccgc acccagcctg
     3361 aagtagtcgc ggatgtccgc ctccgtgcca accccgagcg cacccgccgc ccgcagcacg
     3421 agctcgcgca ccgcctcgtc gtcgtccacc tcgcgcgcca gcacctccgg cggcagcacg
     3481 ttctccacca ggtcgtaatg ccgcgcgaat cccacccgcg tcgccgtcgt cagcaccccc
     3541 gcggcgaaca acgcctccgt cacccacttg gtatcgctgc ggtcccacca cggacccttc
     3601 ttgccgcgct ggccggcgcc gagatgcgac tcgatctggc ctgcggtcga cgggcccagc
     3661 tcggcgaccg cggcgacgac gtcatcggcg agccgcgcat tcttcttgac gatctccttg
     3721 ccccaccggc cgccggtgta ctcccgcatc cgccagcgca gcagcggcca gtcgtcgacc
     3781 gccatcagcg cggcctcatg cgcccaatac tcgacgagca gcctcggcga tcgcgcgctg
     3841 tggctccacg cggcgcggtc caccacatcg cggtcgtacg ggccgagccg gctgaacacc
     3901 ggcgcatagt gcgcccgcac ggccaccgaa accgagtcca actgaagcac ctgaatccgc
     3961 gagaccagcc gcttcagatg cgcccgggtg accggtccac gtggcgcggg ctcatggaag
     4021 ccctgcgcag ccactgcgac ccgacgagcc tgcgcggcgg tgag
//
LOCUS       DASYLX010000634         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3213302,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000634 DASYLX010000000
VERSION     DASYLX010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213302"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGP27_14990"
     CDS             complement(<1..518)
                     /locus_tag="VGP27_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7852637.1"
                     /translation="MDRQGALSALQTAVSAFARRYAAGDNRWCVALSGGPDSLALTAV
                     AAKVKPTTALIVDHRLQQDSSRVAATAREHAIALGCVDAQIICVDVGTAGGPEAAART
                     ARYRALDAARADAQVLLGHTLDDQAETVLLGLGRGSGARSMAGMRPHHPPWGRPLLDV
                     RRAVTHQACDELG"
     gene            complement(497..1561)
                     /locus_tag="VGP27_14995"
     CDS             complement(497..1561)
                     /locus_tag="VGP27_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV7852638.1"
                     /translation="MSRETGGSGKVTVGRTVDWGFAATVGAKLARPGPAATDYTRRQA
                     IEQLSDAALRAEMPVREVTGLNEGGEVSDARIVDRPEWIVAASKSMRTMTGGSETGDK
                     PHFITGRVTGAQTGAVLAFISSGILGQYDPFGDNGGELLLVYPNVIAVERQLRVAPAD
                     FRLWVCLHEVTHRVQFRANPWLADHMSNALAVLTEETGEDVTEVIGRLAGLIRDRRDG
                     VPADPNSTGVLGVLRAVQSEPQQRALDQLLVLGTLLEGHADHVMDAVGPAVVPSVVTI
                     RRRFDERRTRKQPPLQRVLRALLGVDAKMSQYTRGKAFVDHVVGRVGMARFNTIWSGP
                     ETLPLPTEIEEPQRWIDRVL"
     gene            complement(1558..>2179)
                     /gene="dacB"
                     /locus_tag="VGP27_15000"
     CDS             complement(1558..>2179)
                     /gene="dacB"
                     /locus_tag="VGP27_15000"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEV7852639.1"
                     /translation="ALRVDPVTVTVLPSAFDGDEIASVQSPPLLERLGEMMNNSDNVM
                     AESIGREVAAAKGQPQSFESAVRAVLGELDSADIHTDGSHLMDCSGLSIDDRLTAETL
                     DETINAAAGPHQPKLRPLMDLLPIAGGSGTLSNRFLDTDDGRASVGWLRAKTGSLTAT
                     NSLVGVVTDASGRVLTFALISNSAGPTGRTAIDAFAATLRSCGCGT"
BASE COUNT          334 a          783 c          725 g          337 t
ORIGIN      
        1 cccagctcgt cacacgcctg atgggtgacc gcgcgccgga cgtccagcag cgggcggccc
       61 cacggcgggt gatgaggccg catgcccgcc atcgaccttg ctcccgatcc gcgcccgagt
      121 cccagcagca cggtttcggc ctggtcgtcg agcgtgtggc cgagcagcac ctgggcatcg
      181 gcgcgggccg catccaacgc acggtagcgg gccgtgcggg cggccgcttc cggtccgccc
      241 gccgtgccga cgtcgacgca aatgatctgc gcgtcaacac atcccaatgc tatcgcgtgc
      301 tctctggcgg tggctgcgac gcggctggaa tcctgctgca gtcgatggtc gacgatcagc
      361 gcggtggtgg gcttgacttt ggcggcgacg gcggtcagcg ccagcgagtc cggtccgccc
      421 gacaaggcga cgcaccaacg gttgtccccg gcggcgtagc ggcgagcgaa cgccgacacc
      481 gcggtctgca acgcgcttag agcaccctgt cgatccatcg ctgcggctct tcgatttcgg
      541 ttggcagcgg cagtgtttcg ggccccgacc agatggtgtt gaagcgggcc attccgaccc
      601 gaccgacgac gtggtccacg aacgccttgc cgcgggtgta ctggctcatc ttcgcgtcga
      661 cgcccagcag cgcccgcaac acgcgctgca gcggcggctg cttacgggtg cgccgctcgt
      721 cgaaccgccg ccggatggtg accaccgacg gcaccacggc gggcccgacg gcgtccatca
      781 catggtcggc gtgaccctcc agcagcgtgc cgagcaccag caactggtcg agtgcgcgct
      841 gttgcggctc ggactgaacg gcccgcaaca cacccagcac accggtcgaa ttcgggtcgg
      901 caggaacacc atcgcggcga tcacggatca ggccggcaag cctgccgatc acctcggtga
      961 cgtcttcacc ggtttcctcc gtcagcaccg cgagcgcgtt ggacatgtgg tcggccagcc
     1021 acgggttggc gcggaactgc acccgatggg tgacctcgtg caggcacacc cagagccgga
     1081 agtcggccgg cgccacccgc agctgccgct ccaccgcaat cacattgggg tacaccagaa
     1141 gaagctcgcc gccgttgtcg ccgaacgggt cgtactggcc caggatgccc gaggagatga
     1201 aggccagcac cgcacccgtc tgcgcaccgg tgacgcgccc ggtgatgaag tgcggcttgt
     1261 caccggtctc gctgccgccg gtcatcgtcc gcatcgattt cgaggcggcc acgatccatt
     1321 cgggccggtc gacgatgcga gcgtcggaca cctcgccgcc ttcgttcagc cctgtcacct
     1381 cgcgcaccgg catctcggcg cgcagcgccg cgtcggacag ctgttcgatc gcctgccgac
     1441 gggtgtaatc ggtggccgcc ggacccggcc gggccagctt cgccccgacc gtggcggcga
     1501 atccccagtc gacggtgcgg ccgaccgtga ccttgccgga gccgcccgtt tcccggctca
     1561 tgtgccgcac ccgcacgacc gcagggtggc ggcgaacgcg tcgatggccg tccgcccggt
     1621 gggtcccgcg ctgttcgaga tcagcgcgaa ggtcagcacc cggccgctgg cgtcggtgac
     1681 gacgcccacc aaagaattgg tggccgtcaa agacccggtc ttcgcgcgca gccacccgac
     1741 cgacgcgcga ccgtcgtcgg tgtcgaggaa ccggttcgac aacgtcccgc tgccgccggc
     1801 gatcggcaac aggtccatca gcggccgtag cttcggctgg tggggtcccg cggcggcgtt
     1861 gatggtctcg tcgagcgttt ccgcggtgag ccggtcatcg atggacaacc cgctgcagtc
     1921 catcaggtgc gagccatcgg tgtggatgtc ggccgagtcc aactcgccga gcaccgctcg
     1981 cacagcactc tcgaaacttt gcggctgccc tttcgcggca gcgacttcgc ggccgatcga
     2041 ttccgccatc acgttgtcgg aattattcat catctcgccc aaccgctcga gcagcggcgg
     2101 ggactgcacc gaggcgatct cgtcgccgtc gaacgccgac ggcagcaccg tcacggtgac
     2161 cgggtcgaca cgcaatgcg
//
LOCUS       DASYLX010000635         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3763900,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000635 DASYLX010000000
VERSION     DASYLX010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763900"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGP27_15005"
     CDS             <1..393
                     /locus_tag="VGP27_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HEV7852640.1"
                     /translation="GMSKAQGQTSLLGRLSAFFSATMQADSEDRSAAAFLVTSVLEGQ
                     RHPELRSDDHDSMRNSRAFVSWAVNDAIEKGELTTDTDVPQLVEMLVAVMWGMGFYAG
                     YVGSHNELESVVDKLELLLANKLWKLAE"
     gene            486..1445
                     /locus_tag="VGP27_15010"
     CDS             486..1445
                     /locus_tag="VGP27_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852641.1"
                     /translation="MTDLHEAPSSRTEGDSWEITESVGATALGVAAARAVETARDDAL
                     IRDEFAYLLVATAGPQWAQMASSDPEWIAEDEEFRALHEMSRDYQAVRTHYFDDFFHA
                     AMRAGIRQVVILAAGLDSRAYRLQWPAGTTVFEIDQPKVLEYKTSTLDAHGAQPRARR
                     VPVAVDLRNDWPAALADAGFDAAAPTAWLAEGLLPYLPAEAQDRLFQLVTAHSAAGSR
                     IAVEAFSMDLTAQADHQRIARRERSARMRELSGIDIDVDTLTYADGNRADAAGWLADH
                     GWRVDAVPSEVEMTRLGRPAPDYDAEEIASSVLLRAELSGEAR"
     gene            1442..>2230
                     /locus_tag="VGP27_15015"
     CDS             1442..>2230
                     /locus_tag="VGP27_15015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7852642.1"
                     /translation="MSSLRTHDDTWDIASSVGTTAVMVAAARAAETRQADALITDPYA
                     DILVEGAGTGMWATMLDEAIVEKAAAIDPEIAAIFRHMRNYQAVRTHFFDEHFTRATA
                     AGIRQIVILASGLDSRAYRLNWPDGTTVYEIDQPKVLEYKAETLAGNDVKPAATLHQV
                     PVDLRNDWPTALREKGFDSGQPTAWLAEGLLMYLPADAQDRLFTLITELSAPGSRIAA
                     EAVGVSSDERRAQMQERFDTIANELGIVREIDVQELMYNDPDRAD"
BASE COUNT          398 a          746 c          738 g          348 t
ORIGIN      
        1 ggcatgtcga aggcccaggg ccagaccagc ctgctcggcc ggctgtctgc gttcttctcc
       61 gccactatgc aggccgacag cgaggaccgt tcggccgcag cgtttctggt gacctcggtg
      121 ctcgaggggc agcggcatcc cgaactccgc agcgatgacc acgattcgat gcgcaactcg
      181 cgggcgttcg tgtcgtgggc cgtcaacgac gcgatcgaga agggcgaact gaccaccgac
      241 accgacgtcc cgcaacttgt cgagatgctc gtcgcggtga tgtgggggat gggcttctac
      301 gccggctacg tcggttcgca caacgagctc gagtccgtcg tcgacaagct cgaactgctg
      361 ctggccaaca agctgtggaa gctggccgag taactcctct ctggcaattt gctgtgtgac
      421 ctggggtgat gtttcccaca gacgcatata gatcaactaa cttttttagt agcatggctt
      481 aggacatgac tgatctacat gaagcccctt cgagccgtac cgagggcgat agctgggaaa
      541 tcaccgagag cgtcggcgca accgcattgg gtgtcgccgc cgcccgcgcc gtcgagacgg
      601 cgcgcgatga tgcgctgatc cgcgacgagt tcgcctatct gctggtcgcg acggcagggc
      661 cgcagtgggc ccagatggcc agcagcgacc cagaatggat tgccgaggac gaagaattcc
      721 gtgcgcttca cgagatgtcc cgcgactacc aggccgtgcg cacccactac ttcgacgact
      781 tcttccacgc cgcgatgcgg gcgggtatcc gccaagtggt gatcctggcc gccggcctcg
      841 actctcgcgc ataccggctg cagtggccgg cgggcacgac ggttttcgag atcgatcagc
      901 ccaaggtgct ggagtacaag acctcgacgc tggacgcgca cggggcacag ccgcgggcgc
      961 gccgcgttcc ggtggcggtc gatctgcgta acgactggcc tgccgcgctc gccgacgcgg
     1021 gcttcgacgc cgcggcgccg accgcctggc tggccgaggg cctgctgccc tatctgcctg
     1081 ccgaggctca agaccggctc tttcagctcg tcactgcgca cagtgcagcg gggagcagga
     1141 tcgcggtcga ggcgttcagc atggacctca cggcacaggc cgaccaccaa cggatcgcgc
     1201 gccgtgagcg cagcgcccgg atgcgggagc tctcgggcat cgacatcgat gtcgacaccc
     1261 tgacctacgc cgacggcaac cgggcggacg ccgcaggctg gctcgccgat cacggctggc
     1321 gcgtcgacgc cgtgccgagc gaggtcgaga tgacccggct gggccggccg gcccccgact
     1381 acgacgccga agagatcgca tccagcgtgc tgttgcgcgc cgaactgtcg ggagaggctc
     1441 gatgagctca ctgcgcaccc acgacgacac ctgggacatc gcaagcagtg tcgggaccac
     1501 cgccgtgatg gtggccgcgg cgcgcgccgc cgaaaccaga caggccgacg cgctgatcac
     1561 cgacccctac gccgacatcc tggtcgaggg agcgggaacc ggaatgtggg ccacgatgct
     1621 cgatgaggcg atcgtcgaga aggccgccgc catcgacccc gagatcgccg cgatcttccg
     1681 gcacatgcgc aactaccagg ccgtgcgcac ccacttcttc gacgagcact tcacccgggc
     1741 caccgcggcc ggaatccggc agatcgtcat cctggcctcc ggcctcgact cccgcgccta
     1801 ccggttgaac tggcccgacg gcaccacggt ctacgagatc gatcagccca aggttctgga
     1861 gtacaaggcc gagacgttgg cgggcaacga tgtgaagccg gcggcgacgc tgcaccaggt
     1921 gccggtggat ctgcgcaatg actggccgac agcgttgcgc gagaaggggt ttgattccgg
     1981 ccagccgacc gcatggctgg ccgaaggcct gctgatgtat ctacccgccg atgcgcagga
     2041 tcggctcttc acgttgatca ccgagctcag cgcccccggc agccggatcg ccgccgaggc
     2101 ggtcggcgtc agttccgacg aacggcgtgc gcagatgcag gaacggttcg acacgatcgc
     2161 caacgagctc ggcatcgtgc gggagatcga cgtgcaggag ctgatgtaca acgatcccga
     2221 ccgcgccgac
//
LOCUS       DASYLX010000636         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_471563,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000636 DASYLX010000000
VERSION     DASYLX010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471563"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGP27_15020"
     CDS             <1..786
                     /locus_tag="VGP27_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEV7852643.1"
                     /translation="GLVTLVLLVATLALFRGIIGRLAIFLAVVAGYVLALILGDVDTS
                     GIAAAPWIGLPEFHAPTFTVAVLPMFLPAVIALVAENIGHVKSVGQMTDTDVDPLMGR
                     ALAADGVATTLAGFGGGSATTTYAENIGVMAATRIYSTAAYWVAAAVAIALSLCPKVG
                     AVISAVPPGVLGGATIVLYGLVGILGVRIWLTNGVDFGKPLNQMTAAIPLIIGIADFT
                     WQLGDLTFTGIALGSIAALVVYHGMRLLGLRASKAADPTPAEQ"
     gene            786..1565
                     /locus_tag="VGP27_15025"
     CDS             786..1565
                     /locus_tag="VGP27_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HEV7852644.1"
                     /translation="MSARHALVRRPSPRLDAGLITHIARSDDVKVALAQRQWAAYVAA
                     FHGAGWTTHEVPAAPECPDSVFVEDTMVVYADLAVIGRPGADERWPEISAAEDAVSAQ
                     GYRVVRIEAPGTLDGGDVLKHDGTVWVGAGGRTNAEGIAQLTAHLEPFGVEVVTVPVS
                     KALHLKSAVTALPDGTIVGYEAVIDDPGVWGETFLAVPEEPGAHVVLLDNDALLMSSA
                     APRTAKLFEGRGYRVIAVDISEFEKLEGCVTCLSVRLRGRP"
     gene            complement(1567..2742)
                     /locus_tag="VGP27_15030"
     CDS             complement(1567..2742)
                     /locus_tag="VGP27_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852645.1"
                     /translation="MYGLSERDLAIRDTARTFVESLMPHEVEAELAGGLLPKELTAEH
                     SRRAIELGLYATNMPKSVGGHGCTALQQVLVQEQCGRVTNGLAWVMATPPQWWPEVAT
                     DYHRERWLLPSVRGEKHEAYAITEEFAGSDVSELQTTARRDGDEYVINGTKWHVTSFN
                     LADYVFVEAVLTTGEHAGDHVLLVVDVPWPGMEVVRTPHYSHNIADEHPIVAFNDVRV
                     PASHLVGGEGEPMTFTQDWFRFERMMVASRCVGAAQRLVDEMTAFARTREVGGKPLGE
                     YQFVAGMLADSATELFAARTMLYEVARSIDAGRDRKILHGQASMAKLYCSEMAGRVAD
                     RAVQVFGGRGYMRENVAERMFRELRVERIWEGASEIQRIIVARQMMNRGPEAVLEGR"
     gene            2802..>3404
                     /locus_tag="VGP27_15035"
     CDS             2802..>3404
                     /locus_tag="VGP27_15035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7852646.1"
                     /translation="MTSADVVVVGGGTVGAWTAVQLAERGVKRVVLLEKNTLGDGASS
                     RAAGMVRAQGGTEAAIVLGMRSQDFYAASGDRFPLDCGFVAQGYLMPCFSDAEVAQAH
                     DRIALQRKLGLDVEWWSAADVDDRNTGIAPGATLGSSYAPGDGYIDAPRNVLAYTAAL
                     TAQRVDVRERCRFTGLRTAGGRVVGVDTSDGPIDTERVVLT"
BASE COUNT          510 a         1204 c         1129 g          561 t
ORIGIN      
        1 gggctggtca ccctggttct gctcgtcgcc acgctggcgt tgttccgcgg aatcatcggc
       61 aggctcgcga tcttcctcgc cgtggtcgcg gggtacgtgc tggcgctgat cctcggcgac
      121 gtcgacacgt cgggaatcgc cgcggcgccg tggatcgggc tgcccgagtt tcacgcaccc
      181 accttcaccg tggcggtgct gccgatgttc ttgcccgcgg tgatcgcgct tgtcgccgag
      241 aacatcgggc acgtgaagtc cgtcggccag atgacggaca ccgacgtcga ccccctgatg
      301 ggtcgggcgc tggccgccga cggcgtcgcc accacgctcg ccggtttcgg cggcggttcg
      361 gccaccacca cgtacgcgga gaacatcggc gtgatggccg cgacgcggat ctactcgacg
      421 gccgcgtact gggtagccgc cgcggtggcg atcgcactgt cgttgtgtcc caaggtgggc
      481 gcggtgatct ccgccgtgcc gccgggcgtg ctcggcgggg ccaccatcgt gctctacggg
      541 ctggtcggaa tcctcggtgt gcggatctgg ttgaccaacg gcgtcgactt cggcaagccg
      601 ctgaaccaga tgaccgcggc catcccgttg atcatcggca tcgccgactt cacctggcag
      661 ctgggtgatt tgacgttcac cggtattgcg ctcggctcca tcgccgcgct cgtcgtctac
      721 cacggcatgc gactgctcgg cctacgcgcg tcgaaggcgg cagaccccac cccggcggag
      781 cagtgatgtc ggcccgccac gcgctggtgc gccggccgtc accgcggctc gacgccggcc
      841 tcatcacgca catcgcccgc agcgacgatg tgaaggtcgc actcgcacaa cggcaatggg
      901 cggcttacgt cgcggcattt cacggcgcgg gctggaccac ccacgaggtg cccgccgcac
      961 cggagtgtcc cgactcggtg ttcgtcgaag acaccatggt cgtctacgca gatctggcgg
     1021 tcatcggccg tcccggcgcc gacgagcggt ggcccgagat ctccgcggcc gaggacgcgg
     1081 tcagcgcgca gggttatcga gttgtgcgga tcgaagcacc cggcaccctc gacggcggcg
     1141 atgtgttgaa gcacgacggc accgtatggg tcggggcggg cggtcgcacc aatgccgagg
     1201 gaatcgcgca gctgacggcg cacctcgagc cgttcggtgt cgaggtggtg acggtgcccg
     1261 tatcgaaggc actgcacctg aagtcggcgg tcaccgccct ccccgacggc acgatcgtcg
     1321 gctacgaagc cgtcatcgac gacccgggcg tttggggtga gacgtttctc gccgtgcccg
     1381 aggagccggg cgcacatgtc gtcctcctcg acaacgacgc gctgctgatg tcctcggcgg
     1441 ctccgcggac ggcaaagctc ttcgaggggc gcggctaccg cgtgatcgcc gtcgacatct
     1501 ccgagttcga gaagctcgag gggtgtgtca cgtgcctatc cgtccgcctc cgcggccgtc
     1561 cgtgagtcac cgaccctcca gcaccgcctc gggcccgcgg ttcatcatct ggcgagccac
     1621 gatgatccgc tgaatctcgc tggcgccctc ccagattcgt tcgacgcgta gctcgcggaa
     1681 catccgctcg gcgacgttct cacgcatgta gccgcggcca ccgaacacct gcacggcgcg
     1741 gtcggcgacc cgtcccgcca tctccgagca gtacagcttg gccatcgagg cctggccgtg
     1801 aaggatcttg cgatcgcggc ccgcgtcgat ggaccgggcc acctcgtaga gcatcgtgcg
     1861 cgcggcgaag agttcggtcg cgctgtcggc cagcatgccc gccacgaact ggtactcacc
     1921 cagcggcttg ccgccgactt cgcgtgtccg cgcgaacgcc gtcatctcgt cgacgaggcg
     1981 ctgcgccgcg ccaacgcagc gcgacgccac catcatccgt tcgaaccgga accagtcctg
     2041 ggtgaaggtc atcggttcgc cctcgcctcc gacgagatgg ctcgcaggca cccggacgtc
     2101 gttgaacgcg acgatcggat gctcgtcggc gatgttgtgc gaataatgtg gtgtgcgaac
     2161 gacttccatc cccggccacg gcacgtcgac caccagcagc acatggtcgc ccgcgtgctc
     2221 accggtcgtg agcaccgcct cgacgaagac gtagtcggca aggttgaacg acgtcacgtg
     2281 ccacttggtg ccgttgatca cgtactcgtc gccgtcccgg cgcgcggtgg tctgcagctc
     2341 cgagacatcc gagccggcga actcctcggt gatcgcatac gcctcgtgtt tctcaccccg
     2401 cacggacggc agcagccagc gctcgcggtg atagtcggtg gccacctcgg gccaccactg
     2461 tggcggcgtc gccatcaccc aggcgaggcc gttggtgacc cggccacact gctcctgtac
     2521 gagcacctgc tgcaaggcgg tgcagccatg accgcccacc gacttcggca tgttcgtggc
     2581 gtacaggccg agctcgatcg cccgcctcga atgctcggcc gtcagctcct tcggcagcag
     2641 gccgcccgcc agctcggcct ccacctcgtg cggcatcaac gactccacga acgtccgcgc
     2701 cgtatcgcgg atagccagat ctctctccga gagtccatac atccggtgac cccgctcctc
     2761 gctcttgact gagcgttcag tctatgccag cgttccacag tatgaccagt gctgacgtcg
     2821 tagtggtggg tggcggaacg gtcggagcgt ggacggcggt ccagcttgcg gagcgcggcg
     2881 tgaaacgcgt ggtcctgctc gagaagaaca cgctgggcga cggagcaagc agccgtgcgg
     2941 cgggcatggt gcgcgcccag ggcggcaccg aagccgccat cgtgctcggc atgcggagcc
     3001 aggatttcta cgccgccagc ggcgaccggt tcccgctcga ctgcggattc gtcgcccagg
     3061 gctacctgat gccgtgcttc agcgacgccg aggtggcgca ggcccacgac cgcatcgcgc
     3121 tgcaacgcaa gcttggactc gacgtcgagt ggtggtccgc cgccgacgtc gacgaccgca
     3181 acaccggcat cgcgcccggc gccaccctgg gctcgtcgta cgcgcccggt gacggataca
     3241 tcgacgcacc gcgcaacgtg cttgcctaca ccgcggcgct gacggcccag cgggtcgacg
     3301 tccgcgagcg ctgccggttc acgggactgc gcaccgcggg cggccgggtg gtcggcgtcg
     3361 acacgtccga cggcccgatc gacaccgaac gcgtcgtgct cacc
//
LOCUS       DASYLX010000637         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1238414,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000637 DASYLX010000000
VERSION     DASYLX010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238414"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGP27_15040"
     CDS             complement(<1..344)
                     /locus_tag="VGP27_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HEV7852647.1"
                     /translation="MGIEYESVVDHPLGEVFAWHTRPGAMSRLVPPWQPMSVVAETES
                     LADGQAVLGLPGGLRWIAQHDRDGFDPPHRFVDVLSSAGPRSLPPRVIGWWRHTHEFS
                     EAPGGGTTVRDEV"
     gene            441..1118
                     /locus_tag="VGP27_15045"
     CDS             441..1118
                     /locus_tag="VGP27_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEV7852648.1"
                     /translation="MAGWSGLARKSARRTFRDRREAGRVLAQGLAAYHGRDDLLILGL
                     ARGGVPVGWEVAAALGAPLDVFLVRKLGVPQWEELAMGALASGGGLVMNDNLMKSLHI
                     SDKALRATIDRESEELNRRELAYRGDRPPADPKGLVAIIVDDGIATGASMLAAVRAVR
                     AAQPASVVVAVPVGPPSACKQLGREADDVVCAMMPTAFEAVGQVYADFHQVSDDEVRE
                     LLATPTR"
     gene            complement(1115..1432)
                     /locus_tag="VGP27_15050"
     CDS             complement(1115..1432)
                     /locus_tag="VGP27_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7852649.1"
                     /translation="MLIVALVLAVIGLAALVTAVVTSNELIAWVCIAASVIGVVLLIV
                     DALRERSRGSADAPAQAPDEEFDEEYPDEPAAHADAASTEAPAAEPVDSDVAEVVEED
                     RPR"
     gene            complement(1464..2276)
                     /locus_tag="VGP27_15055"
     CDS             complement(1464..2276)
                     /locus_tag="VGP27_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEV7852650.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLVE
                     ENADLRQRLAELEQELASARAGGGGQPTQAIPLYQPEPEPEPAPQPVYEAPPQAPPAP
                     VSEDQHLKAARVLSLAQDTADRLTSTAKAESEKLLSDARAQADQMVGEARATAESTVT
                     EARQRADSMLADAQSRSETQLRQAQEKADALQADAERKHSEIMGTINQQRTVLEGRLE
                     QLRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSSANNDGGGFNQFNRGNN"
     gene            complement(2458..2748)
                     /locus_tag="VGP27_15060"
     CDS             complement(2458..2748)
                     /locus_tag="VGP27_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV7852651.1"
                     /translation="MALFFEILGFALFVFWLLLIARVVVEFIRSFSRDWHPRGVTVVI
                     LEVIMTLTDPPVKLLRRLIPQLTIGAIRFDLSIMVLLLVAFIGMQLAFSAAA"
     gene            complement(2854..3462)
                     /locus_tag="VGP27_15065"
     CDS             complement(2854..3462)
                     /locus_tag="VGP27_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV7852652.1"
                     /translation="MSTLHKVKAYFGMAPMDDYDDEYYDDEDRPSRPHPRRERFADEG
                     YGRYDDRYDDEEMPGAYRGGYADEGRFHPREFDRAAPRFAPLRGSTRGALAMDPRRMA
                     MLFDESSPLSKITTLRPKDYSEARTIGERFRDGTPVIMDLVTMDNADAKRLVDFAAGL
                     AFALRGSFDKVATKVFLLSPADVDVTPDDRRRIAETGFYAYQ"
     gene            complement(3517..4266)
                     /locus_tag="VGP27_15070"
     CDS             complement(3517..4266)
                     /locus_tag="VGP27_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV7852653.1"
                     /translation="MTELATTREVELADALTLVRERLAKAADAAGRNNGEIELLPITK
                     FFPATDVLILAHLGCDAFGESREQEAAAKSAECNSAEPHSPIRWHMVGRIQRNKARSI
                     ANWAYAAHSVDSAKLIAALDRASSDALAAGGRSDPLQVYIQVSLDGDVARGGVDVARR
                     DQVDELCAQVHAAPGLEFVGLMGIPPLGADADQAFARLQAEHRRVQGFYQHRLGLSAG
                     MSSDLEAAVEHGSTCVRVGTAVMGQRPLTSP"
     gene            complement(4263..4970)
                     /gene="pgeF"
                     /locus_tag="VGP27_15075"
     CDS             complement(4263..4970)
                     /gene="pgeF"
                     /locus_tag="VGP27_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV7852654.1"
                     /translation="MSVRVRRVTTTRAGGVSAPPYQTFNLGDHVGDDPAAVATNRKRL
                     AAAIGLGADAVVWMNQVHGDRVARIDGPIGSAVDGTDALVTTTRRLALAVVTADCVPV
                     LMSDARAGVVAAVHAGRVGAQNGVVIRAVEAMRDAGAHADDISVLLGPAVSGRNYEVP
                     ATMADEVEAALPGSRTTTAQGTPGLDLRAGIARQITGLGITSIDIDPRCTVDDRALFS
                     HRRDAPTGRLASVVWME"
     gene            complement(5025..>6009)
                     /gene="ftsZ"
                     /locus_tag="VGP27_15080"
     CDS             complement(5025..>6009)
                     /gene="ftsZ"
                     /locus_tag="VGP27_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV7852655.1"
                     /translation="DADVKLDVGRDSTRGLGAGADPEVGRKAADDAKDDIEELLRGAD
                     MVFVTAGEGGGTGTGGAPVVATIARKLGALTVGVVTRPFSFEGKRRSNQAETGIASLR
                     ESCDTLIVIPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFAD
                     VKGIMSGAGTALMGIGSARGDGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLG
                     LFEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDAAGPSRKPVVGNG
                     QAIAPGKAGKVTSSLFEPADAASVPVHTNGATVSIGGDDDDDVDVPPFMRH"
BASE COUNT          968 a         2031 c         1989 g         1021 t
ORIGIN      
        1 acctcgtcgc gcaccgtggt gccgcccccc ggtgcctccg agaactcgtg ggtgtgccgc
       61 caccagccga tgacacgcgg cggcagcgat cgcggtccgg ccgaggacag cacgtcgacg
      121 aaccggtgcg gcggatcgaa accgtcccta tcgtgctggg cgatccagcg caggccgccc
      181 ggcaggccca gcaccgcctg accatcggcc agcgactcgg tttcggcgac caccgacatc
      241 ggctgccacg gcggaacgag ccggctcatg gcgcccggcc tggtgtgcca ggcgaacacc
      301 tcgcccagcg ggtggtcgac gacgctctcg tattcgatgc ccatggttcc ttccggcttg
      361 gttccagtga ttcggagtcg atgcggtttt ggattgcccg tcgtaggctg aaccagtccc
      421 cgaaatggga agacgcagtc atggccggtt ggagtgggct cgcccgaaaa agcgcgagac
      481 gtaccttccg ggaccgccgt gaagccggcc gggtcctggc tcaagggttg gcggcctatc
      541 acggcagaga cgatctcctg atcctgggcc tggcccgcgg cggcgtgccc gtcggctggg
      601 aggtcgcggc cgcgctcggg gcaccgctcg acgtcttcct cgtccgcaag ctcggcgtgc
      661 cgcagtggga agagctcgcg atgggcgccc tggccagtgg cggtggcctg gtgatgaacg
      721 acaacctgat gaaaagcctc cacatcagcg acaaggcgct gcgcgccacg atcgaccgcg
      781 agtccgagga gctcaaccgg cgcgagctgg cctaccgggg cgaccggccg cccgccgacc
      841 ccaagggctt ggtcgccatc atcgtcgacg acggaatcgc cacgggcgca agcatgctcg
      901 ccgccgttcg cgctgtgcgg gccgcacagc cggcaagcgt cgtggtcgcg gttcccgtcg
      961 ggccaccatc ggcctgcaag caactcggcc gggaggccga tgacgtggtg tgtgcgatga
     1021 tgccgaccgc gttcgaggcg gtcggacagg tgtacgccga cttccaccag gtctccgacg
     1081 acgaagtgcg cgaactgctg gcgacgccga cgcgttagcg cgggcggtct tcctcgacga
     1141 cctctgcgac atcgctgtcg acgggttcgg cggcgggggc ttcggtgctt gcggcgtccg
     1201 cgtgagccgc cggctcgtcc ggatactcct cgtcgaactc ctcgtcgggc gcctgcgccg
     1261 gggcgtccgc gctaccgcgg gagcgctccc tcagcgcatc gacgatcaac agcacgacgc
     1321 cgatcacgct cgccgcgata cagacccaag cgatcagctc attgctcgtg acgaccgcgg
     1381 tcaccagggc cgccaggccg atgacggcga gcacgagggc aacgatcaac attggtcaat
     1441 cccttcgccg aggggcttgg gcctcagttg ttaccgcggt tgaactgatt gaagccgccg
     1501 ccgtcgttat tggcgctcga gtcgaccggt gcggcggacc cacgctgacc gagttcttcg
     1561 agctgagact ccagataggt cttgagccgg gtacggtact cgcgttcgaa cgtgcgcagc
     1621 tgttccagcc ggccttcgag cacggtgcgc tgctgattga tcgtgcccat gatctcggag
     1681 tgcttgcgct cggcgtccgc ctgcagcgca tcggccttct cctgagcctg acgcagctgg
     1741 gtctccgagc gggactgggc gtcggcgagc atcgagtccg cccgctggcg cgcctcggtc
     1801 acagtggact ccgcagtggc ccgcgcctcg ccgaccatct ggtctgcctg ggcccgcgca
     1861 tccgacagca gcttctccga ctcggccttg gcggtcgagg tcaaccggtc ggcggtgtcc
     1921 tgggccaagc tgagcacgcg ggcggccttg aggtgctggt cctcactgac aggagcgggc
     1981 ggcgcctgcg gcggcgcctc atatacgggc tgcggtgcgg gctcgggttc gggctcgggc
     2041 tgatacagcg ggatggcctg cgttggctgt cctccaccgc ctgcgcgcgc gctcgccaac
     2101 tcctgctcca gctccgccag tcgctgacgg aggtcggcgt tctcttccac gaggcgggtc
     2161 agctcgtttt cgaccagatc gaggaacgca tcgacctcgt cctcgttgta gccgcgcttg
     2221 ccgatgggcg gcttactgaa cgcgacgttg tgcacgtcgg ctggagtgag cggcattttc
     2281 tgccccctag atggtctgga ctgtcaatcg atctcaaagt gtagaacgtc agtggagtaa
     2341 ctggcgccca tcctgtcaca ccagaccctg cggttgcggg cgaggccgat aattaagagc
     2401 gaaattcaaa cttcttatgc ccaattcgac gcttggcaaa cggcgaggct tgtggcgtta
     2461 agccgcggcg ctgaacgcga gttgcatacc gatgaaggcg acgagcagca gtaccatgat
     2521 cgacaggtcg aatcggatcg cccctatcgt gagctgcgga atgagccgtc gcagcagctt
     2581 caccggcgga tcagtcagcg tcatgatcac ctcgaggatc acgacggtga cgcccctggg
     2641 gtgccagtcg cggctgaacg agcggatgaa ctcgaccacc actcgggcga tgagcagcag
     2701 ccagaatacg aacagcgcaa aacccagaat ctcaaagaac agggccaact aagccgacct
     2761 cactacagcc gatgtgactt tcgatacagg ccgcgggccg atgccggccg gcgcgatgtc
     2821 gccagcctac ccgccccgcg ccgaccggcg gacctactga taggcgtaga agccggtctc
     2881 ggcgatccgt ctgcgatcgt cgggagtgac gtcgacgtcg gcgggtgaga gcagaaacac
     2941 cttggtggcg accttgtcga aggagccacg gagggcgaat gccaggcccg cggcgaagtc
     3001 gacgagccgc ttggcgtcgg cgttgtccat ggtgaccagg tccatgatca ccggggtgcc
     3061 gtcgcggaag cgctcaccga tggtgcgggc ctcgctgtag tccttgggcc gcagcgtggt
     3121 gatcttcgac agcgggctgc tttcgtcgaa cagcatcgcc atccggcgcg gatccatggc
     3181 aagagcgccg cgggtcgagc cgcgcagcgg cgcgaacctc ggcgcggcac gatcgaactc
     3241 gcgcggatgg aagcgtccct cgtcggcgta cccgccgcgg taggcgcccg gcatctcctc
     3301 gtcgtcgtaa cggtcgtcgt agcggccgta cccctcgtcg gcgaaacgct cacggcgcgg
     3361 atgagggcga gagggacggt cctcgtcgtc gtagtactcg tcgtcgtagt cgtccatagg
     3421 cgccatcccg aagtacgcct tgaccttgtg cagtgtgctc atcgctggac ccttcttgcg
     3481 tgatgcctgt gatgaagatg tgactggagt gactactcat ggtgacgtta gaggacgttg
     3541 tcccattacc gcggtaccga cacgcacaca cgtcgaacca tgttcgaccg ccgcctcgag
     3601 gtcgctggac atccctgccg acagcccgag gcggtgctga taaaaaccct gtacccgccg
     3661 gtgttcggcc tgcaaacgcg cgaatgcctg gtctgcgtcg gcgccaagcg gcggtatgcc
     3721 catcaggccg acgaactcca acccgggcgc ggcgtgcacc tgcgcacaca gctcgtccac
     3781 ctgatcccgg cgcgcgacgt cgactccccc gcgcgccacg tcgccgtcga gactgacctg
     3841 gatgtagacc tgcagcggat cggaccgccc gcctgctgcg agcgcatcgg acgacgcccg
     3901 gtccaatgcg gcgatcagct tggcgctgtc gaccgagtgc gccgcgtacg cccagttggc
     3961 gatcgaccgg gccttgttgc gctgaatccg gccgaccatg tgccagcgaa tcggcgaatg
     4021 gggctcggcg gaattgcatt cggccgattt cgcggccgct tcttgttccc gcgattcacc
     4081 aaaagcatca caacctaaat gcgccagaat aagcacgtcg gttgctggaa agaatttcgt
     4141 aatcgggagt aattcaattt cgccattgtt tcgtcccgcc gcatccgctg ccttcgccag
     4201 ccgttcgcga acgagggtca gcgcatcggc cagttcaacc tctcgcgtgg tagccaactc
     4261 ggtcattcca tccacaccac tgacgcgagc cgtccggtcg gggcgtcgcg gcggtggctg
     4321 aacagggcgc ggtcgtcgac cgtgcatcgc ggatcgatat cgatggaggt gatgcccaaa
     4381 cctgttattt gcctggcgat tccggcccgt aagtcaagcc cgggggtgcc ttgggcagtg
     4441 gtggtcctgc tgccgggcag ggcggcttcc acctcgtcgg ccatcgtcgc aggcacttcg
     4501 tagttgcggc cgctgaccgc cggaccgagc aggacggaga tgtcatcggc gtgagccccg
     4561 gcgtcacgca tcgcctcgac ggcgcggatg acgacgccat tctgcgcacc gactcggccc
     4621 gcatgcaccg cagcaaccac ccccgcccgg gcgtcggaca ttaaaacggg aacgcaatcg
     4681 gcggttacga cggccaatgc caatcgccgt gtggtagtga ccaatgcgtc ggtcccgtcg
     4741 acggcggacc cgatcgggcc gtcgatccgt gccacccggt cgccgtgcac ctggttcatc
     4801 cagaccaccg cgtcggcgcc cagcccgatc gcggccgcca gccgcttcct gttggtcgcg
     4861 acggccgccg ggtcgtcgcc gacgtgatcg ccgagattga aagtttggta gggcggcgcc
     4921 gagacaccgc cggcgcgggt ggtcgtcacg cgccgaaccc gaacactcac gaccccagta
     4981 tcaggggcgc gcacgagagg agcaccaccg agtggaatcg gctgtcagtg ccgcatgaac
     5041 ggcggaacgt cgacgtcgtc gtcatcatct ccgccgatgc tgaccgtcgc gccgtttgtg
     5101 tgcaccggca cactggcggc atcggccggt tcgaagagcg acgaggtcac cttgcccgcc
     5161 tttcccggcg cgatcgcctg gccgttgccc accaccggct ttcggctcgg gcctgcggcg
     5221 tcgaagcccg ccgcgatcac cgtcacccgc acctcgtcgc cgagcgagtc gtcgatcacg
     5281 gtgccgaaga tgatgttggc gtcggggtgc gcggcatcct ggaccagcga ggcggcctcg
     5341 ttgatctcga acaaacccag gtcgctgccg cccgcgatgg acatcagcac gccctgcgca
     5401 ccttccatgg acgcctcgag cagcggcgag ttgatcgcga tctcggcggc cttgagcgac
     5461 ctgccgtcgc cgcgcgccga gccgatgccc atcaacgccg tgcctgctcc ggacatgatg
     5521 cccttgacgt cggcgaagtc gacgttgatc aggccggggg tggtgatcag gtcggtgatg
     5581 ccctgcacac cgttgaggag tacctcgtcg gcactgcgga acgcgtccat caaggacacc
     5641 gcggcgtcgc ccatctgcag caggcggtcg ttggggatca cgatcaacgt gtcgcagctc
     5701 tcccgcaggg aggcgatgcc cgtctcggcc tggttgcttc gccgcttgcc ctcgaatgag
     5761 aacggccttg tcaccacgcc gaccgtgagt gcgcccagct tgcgcgcgat ggtcgcgacg
     5821 acgggcgccc cgccggttcc tgttccaccg ccctcacccg cggtcacgaa caccatgtcg
     5881 gcgccgcgca gcagctcctc gatgtcgtcc ttggcgtcat cggcggcctt gcgaccgacc
     5941 tcgggatcgg cacctgcgcc gagcccgcgg gtcgagtcac ggccgacgtc gagcttgacg
     6001 tcggcatcg
//
LOCUS       DASYLX010000638         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3105658,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000638 DASYLX010000000
VERSION     DASYLX010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105658"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGP27_15085"
     CDS             complement(<1..264)
                     /locus_tag="VGP27_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7852656.1"
                     /translation="MSNVPVIGLNNGNYIPQMGFGVFQIAPERTAEAVKAAVEIGYRH
                     IDTAEMYGNEKGVGQGVRDAGLDRADVFITSKLNNGFHKPDDAR"
     gene            836..1186
                     /locus_tag="VGP27_15090"
     CDS             836..1186
                     /locus_tag="VGP27_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852657.1"
                     /translation="MRIKLNYVAPLLAAGAAAVAIAAAPIAAAAPAPAAAPHGACDAG
                     VSGSICDTAGDSGINDSPPPVSYDPYGGEGFLLGYGGGFHGGFGGGGFGGGGFHGGGG
                     GGGHGGGGGGGHGR"
     gene            1282..1773
                     /locus_tag="VGP27_15095"
     CDS             1282..1773
                     /locus_tag="VGP27_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV7852658.1"
                     /translation="MTTSRRLTDDQVREFDAPRSLPLNLQKVLVDLIELHLQGKQAHW
                     NLVGTNFRDLHLQLDEIVDTAREASDTIAERMRSLNAVPDGSSDTVAASTTLPAISRA
                     ELSTTETVDLITTRIYATVGTMRFVHDEVDSEDPSTADLLHQMIDSFEKAAWMLKSEN
                     RKI"
     gene            complement(1790..2179)
                     /locus_tag="VGP27_15100"
     CDS             complement(1790..2179)
                     /locus_tag="VGP27_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7852659.1"
                     /translation="MAFDPGAIARAFSEHRFDEALGFLAKDVRWTIVGYMVLEGADAV
                     RQTCRETAASLEDTTTTYDRCVVADAADVVVVDTFARYSGPGGVTAVASCDIYEFVGD
                     EITAITSYAVEVDPESAGSQPPPRDTS"
     gene            complement(2252..2440)
                     /locus_tag="VGP27_15105"
     CDS             complement(2252..2440)
                     /locus_tag="VGP27_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852660.1"
                     /translation="MIADDIGDWDAAYLLGALSLEDQCTYEAFIGAKPERAAVLSELA
                     GLLGRLSVLSRDEALVLI"
     gene            complement(2437..2949)
                     /locus_tag="VGP27_15110"
     CDS             complement(2437..2949)
                     /locus_tag="VGP27_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7852661.1"
                     /translation="MTDPEQSAVLRAIHDAHSQALLRYVLRLTRGDMAFAEDVVQESL
                     LRLWRKPDLMRQCTDGVRAWLFTVARNQVIDDRRSAGYARELQTESVPQRRSPDEIGP
                     AFDKCLVSDALMSLSADHRVAIVRAYYLGQTVATIAEYERIPEGTVKSRLHYALHALR
                     NALQETGAVC"
     gene            complement(3040..4242)
                     /gene="hemW"
                     /locus_tag="VGP27_15115"
     CDS             complement(3040..4242)
                     /gene="hemW"
                     /locus_tag="VGP27_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEV7852662.1"
                     /translation="MTVRTQPVDLPCLAPTRGRPFGLYIHVPFCATRCGYCDFNTYTP
                     AELGGVNARADERSTESDRWLVALRAELAEAAVRLGAAPPVQTVFVGGGTPSLLGGAG
                     LTRVLESVREHFVLARDAEVTTEANPESTSPELFAQLRAAGYTRVSLGMQSVAPHVLA
                     VLDRVHSPGRAPAAAREALAAGFDHVNIDLIYGTPGETDDDLLRSIDAAVEGGVDHIS
                     AYALTVEHGTAMGRRVRRGEMSPTDDDVMAHRYELADRRLRAAGFDWYEVSNWCTPGG
                     ECRHNIGYWDGGEWWGAGPGAHGYVGSTRWWNIKHPNGYAQALAEGRLPVADFEQLSA
                     VATHTEDVMLRLRLRHGLSADSLSDRERDRAQIAIGDGLLRVEAEQLVLTDRGRLLAD
                     AVVRTVLD"
BASE COUNT          839 a         1560 c         1429 g          803 t
ORIGIN      
        1 gcgcgcatcg tctggcttgt ggaagccatt gttgagcttg ctcgtgatga agacatcggc
       61 gcggtccaat ccggcgtcgc ggacgccctg gccgaccccc ttctcgtttc cgtacatctc
      121 tgcggtgtca atgtgccggt agccgatctc gacagcagcc ttcaccgctt cagcggttct
      181 ctctggcgct atctggaaga caccaaaacc catctgcggg atgtagtttc cgttgtttag
      241 tccgattaca ggaacattgc tcatatcatc tctcccagat tggatcggtg cgacggtgaa
      301 actaggtcct gtgccgtgtt gccggcgaca gccagagtcc gcttgttgcg gcggaatccc
      361 aattgagcgg taacgcttag cggccccact gcggcatcga cgacgaacga ccagcgcccc
      421 gatgggtggg gactacgcgt ggcttcggct ttgaacggcg atacggcgca gatcatcgtg
      481 tcggtcgggt cctcccacat cgctacacct ctcgtttcct cgcgaatgcc tcggtagcgc
      541 cgaaatcgct tcccaccaag cttgcatttc cgggtggtca ctgtcgtccc cgctggcacc
      601 cactgaacat tctggtcagc accccctgca tgaccgacca gaagcaaaca tttatccgac
      661 caggacgagg ggatttcgcg cgtccgagtc ggtgaggcgc cttggccctt ggcggccatg
      721 ggctgattgc agctgggggg aaacaggttt gggtggcatc cgtgatgacg cggactcggt
      781 actgcgaaat cctggaatga gcctgatcag ggcccaacca ttgaggagca cgaccttgag
      841 gatcaaactg aattacgtcg caccactgtt ggcggcggga gctgccgcgg tggcgatcgc
      901 cgccgccccg atcgccgcgg ccgctccggc tccggctgcg gctccgcacg gggcctgcga
      961 cgccggagtc tccggcagca tttgtgatac agccggcgac tccgggatca acgactcccc
     1021 acccccggtt agctacgatc cctacggagg cgagggcttt ctgctcggct acggcggtgg
     1081 ttttcacggc ggcttcggcg gtggcggctt cggcggcggc ggctttcacg gcggcggtgg
     1141 cgggggcgga cacggcggcg ggggcggggg cggacacggc cggtagcggt cacgggccac
     1201 gcctggcagc caaaagacac catgaaacgt tgtgcgccag catcattggc tttccacaac
     1261 catgactaga atggagatgc aatgactact tcacgccgtc tgaccgatga tcaggtccga
     1321 gagttcgacg ccccgcgcag ccttccgctg aacctacaga aggttctagt cgatctcatc
     1381 gagctgcatc tacagggtaa acaggcccat tggaatctcg tcggcaccaa cttccgggat
     1441 ctgcacctgc aactcgacga aatcgtcgac accgcacgcg aagccagtga caccattgcc
     1501 gagcgtatgc ggtcgctcaa cgcggtgccc gacggcagct ccgacaccgt cgccgcgtcc
     1561 accacactgc ccgccatctc gcgggcggaa ctcagcacca cggaaacggt cgatctcatc
     1621 accacccgga tctacgccac cgtcggcacc atgcgcttcg tccacgacga ggtggactca
     1681 gaagacccgt ccaccgccga tctccttcac cagatgatcg acagctttga gaaggcggcc
     1741 tggatgttga agtcggagaa ccggaaaatc tgactctgtc gggatgccct caagacgtgt
     1801 cccgaggcgg cggctgcgag ccggcggatt cagggtccac ctcgaccgca taggacgtga
     1861 tcgcggtgat ctcatcgccc acgaattcgt agatgtcgca actggcgacc gccgtcacgc
     1921 caccggggcc gctgtaacgc gcaaaggtgt cgaccacaac gacgtccgcc gcgtccgcca
     1981 ccacacagcg gtcgtaggtt gttgtggtgt cttcgaggct ggccgcggtc tcgcggcagg
     2041 tctgccggac ggcatcagcc ccctccagga ccatgtagcc cacgatcgtc cagcggacat
     2101 ccttggcgag aaagccgagc gcctcgtcga accgatgctc ggaaaaagct cgggcgatag
     2161 cgccggggtc gaaagccatg aaccgacgct actgccatcg cacttcggga ttgccgaccg
     2221 ctcgtgagtc ccgcttcgcc gcgggctagc gctagatcag gacgagcgct tcgtcgcggc
     2281 tcagcacgct gagcctgccc agcaggccgg ccaactcgct cagcaccgcg gcccgctccg
     2341 gtttcgcgcc aataaaggcc tcgtaggtgc actggtcctc cagactcagg gcgcccagaa
     2401 gataggccgc gtcccaatcg ccgatgtcgt ccgcgatcaa cagacagcgc ccgtttcctg
     2461 caaggcgttt ctcagtgcgt gcagcgcgta atgaagccgg gatttgacgg tcccctccgg
     2521 aatccgttcg tattcggcga tggtggccac cgtttggccg agatagtagg cacgaacgat
     2581 cgcaacgcga tggtcggccg acaaggacat cagcgcatcc gacacgagac acttgtcgaa
     2641 agcgggaccg atctcgtccg gcgagcgccg ttggggaacc gactccgtct gcaattcccg
     2701 cgcatatccc gcacttcggc gatcgtcgat cacctggttg cgggcgacgg tgaacagcca
     2761 ggccctgacc ccgtcggtgc actgccgcat caggtcgggt ttacgccaaa gccgcagaag
     2821 cgattcctgc acgacgtcct ctgcgaacgc catgtcgccc cgggtgagcc gcagaacata
     2881 gcgcaacagc gcctggctgt gtgcgtcatg aatcgcccgc aagactgcgg actgctcggg
     2941 atcggtcacc ccgcacatgc tccgcgcgcg cccttggcat ccgctgtgaa ttgacttgtg
     3001 aatacgagac agaacgcgga aatcgttacg atgtgtggct cagtcgagca cggtgcgcac
     3061 gacggcatcg gccagaagtc ggccgcggtc ggtgagcacc agctgctcgg cttcaaccct
     3121 cagcaaccca tcgccgatgg cgatctgcgc gcggtcgcgt tcgcgatcgc tgagagaatc
     3181 ggccgacaat ccgtgtcgca accgcagccg aagcatcaca tcctcggtgt gagtcgcgac
     3241 ggcgctgagc tgttcgaaat cggccaccgg aagccgtcct tcggccagtg cctgcgcata
     3301 tccgttgggg tgcttgatat tccaccaccg ggtcgatccg acgtagccgt gcgcccccgg
     3361 acctgcgccc caccactcgc cgccgtccca gtagccaatg ttgtgtctgc actcgccacc
     3421 cggcgtgcac cagttggaca cctcatacca gtcgaaaccg gctgcacgca gtcgtcggtc
     3481 ggcaagctcg tagcgatgtg ccatcacgtc gtcatcggtc ggcgacatct cgccgcggcg
     3541 cacccgacgg cccatcgccg tgccgtgctc gacggtcagc gcatacgccg agatgtgatc
     3601 aacgcctcct tccaccgcgg cgtcgatcga ccgcagaagg tcgtcgtcgg tctccccggg
     3661 ggtgccgtag atgaggtcga tgttgacgtg atcgaacccc gccgcaaggg cctcgcgcgc
     3721 cgccgcaggc gcccggcccg gcgaatgcac ccggtcgagc accgccagca catgaggcgc
     3781 caccgactgc atgccgagcg acacccgcgt gtagccggcg gcgcgcagct gcgcgaagag
     3841 ttccggagac gtcgactccg gattggcctc ggtggtgacc tcggcgtcgc gcgccagaac
     3901 gaagtgctcc ctgaccgatt ccagcaccct ggtcaggcct gcaccgccga gcagcgacgg
     3961 cgttcctccg ccgacgaaca ccgtctgcac gggcggagcc gcaccgagcc ggaccgcggc
     4021 ctcggcgagc tcggcccgca gggcgaccag ccagcgatct gattcggtgc tcctctcgtc
     4081 cgctcgggcg ttcacgccgc ccagctcggc cggcgtatag gtgttgaagt cgcagtatcc
     4141 gcaacgggtg gcgcagaacg gcacgtggat atagaggccg aacggcctgc cccgcgtcgg
     4201 cgcaaggcag ggcaggtcga ctggctgggt tcgaaccgtc acacaaccag ttttacagag
     4261 gccttttgct caccctgagc gcaaatatgg cccgtttagg gcatgtgtcg atgtccgtgg
     4321 cacaattggg gccgtgaccg ccgcccgcat cttcccccac cgcgtatcgc tggtggcacg
     4381 gcggcatgtc gactacaagc gcgtctgtac ctcttgttgt ctgccttgat gtcgtagacc
     4441 tgcccaccag tacttcagct ggccgccgga tctgcgccgc cggacagcca ccggtgaaca
     4501 gcgcgcccga aggccggctc ccgataggag ccacatcaga tgaccaccgc caagccggcg
     4561 aagcccgcca agcgcagggg tgaaggccag tggaagctcg gctaccgcga gcccctgaac
     4621 cccaatgagc a
//
LOCUS       DASYLX010000639         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3653100,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000639 DASYLX010000000
VERSION     DASYLX010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653100"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VGP27_15120"
     CDS             complement(<1..1379)
                     /locus_tag="VGP27_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7852663.1"
                     /translation="MATFRPPHLRPSSIPEPSSLSLRWRVMLLAMSMVAMVVVLMALA
                     VYVVVSRALYADIDNQLHSRAQLLIESGSLAADPGKAIEGTAYSDVNAMLVNPGRSIY
                     TANQQGQTLPIGKPEKDVMSGQLLLSLRTADHQRVLAVHLNNGSSLLISKSLAPTGKV
                     LKRLGTVLLIVGGIGVAIAAFAGGMVARAGLRPVARLTEAAERVARTDDLRPIPVVGS
                     DELARLTQAFNMMLRGLAESRERQARLVTDAGHELRTPLTSLRTNVELLMASQEPGAP
                     RLPEEDLAELRTDVIAQIEELSTLVGDLVDLTRDEAGGVVHESVDISEIVDRSLERVR
                     RRRNDIDFDVHVIPWQVYGDAAGLSRAVLNLLDNAAKWSPPNGRVGVRLAQTDPVHAE
                     LVVSDHGPGIPPHERQLVFDRFFRSTSARAMPGSGLGLAIVKQVVHKHGGALRVDDTV
                     RGGQPPGTS"
     gene            complement(1456..>1583)
                     /locus_tag="VGP27_15125"
     CDS             complement(1456..>1583)
                     /locus_tag="VGP27_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV7852664.1"
                     /translation="TSGNALEVYVGYLRRKTEAEGEPRLIHTVRGVGYVLRETPP"
BASE COUNT          261 a          508 c          549 g          265 t
ORIGIN      
        1 atagaggtgc ccggcggctg gcctccgcgc acggtgtcgt ccacccgaag cgcgccaccg
       61 tgtttgtgta cgacttgttt gacgatcgcc agaccaaggc ccgagccggg catggcccgc
      121 gcggaggtgg accggaagaa ccgatcgaaa acgagctggc gttcgtgcgg tgggatgccg
      181 ggtccgtggt cggagacgac gagctcggcg tgtacggggt cggtctgggc caggcgcacg
      241 ccgacccgtc cgttgggcgg actccacttg gcggcgttgt cgagcaggtt cagcacggcg
      301 cgcgacaggc cggccgcatc gccgtacacc tgccacggga tgacgtggac gtcgaagtca
      361 atgtcgttgc ggcgccgccg aacccgctcg aggctgcggt cgacgatctc ggatatgtcg
      421 acagactcgt gcaccacacc gcccgcctcg tcgcgggtga ggtccaccaa atcgcccacc
      481 agcgtcgaca attcctcgat ctgggcgatg acgtcggtgc gcagttcggc caagtcctcc
      541 tcgggtaggc gcggcgcgcc gggttcctgg gaggccatca ggagttcgac gttcgtgcgc
      601 agcgacgtca gcggggtgcg cagctcgtga cccgcgtcgg tgaccaggcg ggcctggcgc
      661 tcgcgcgact cggccagtcc gcgcaacatc atgttgaaag cttgtgtgag acgggcgagt
      721 tcgtcgctac ccactacggg tatcgggcgc aggtcatcgg tgcgggcgac ccgttcggcg
      781 gcctcggtga gtctggctac gggtctcaag cccgcacggg ccaccatgcc gcctgcgaac
      841 gcggcgatcg ccaccccgat accgccgacg atgagcagca ctgtgccgag gcgtttgagc
      901 accttgccgg ttggggccag gctcttggag atcaacagcg aactgccgtt gttcaaatgg
      961 acggcaagta cccgctggtg atcggccgtg cgcagcgaca gcagcagttg gccggacatc
     1021 acgtccttct ccggcttgcc gatgggcagc gtctgcccct gctggttggc ggtgtagatg
     1081 gagcggccgg ggttcaccag catcgcgttg acgtcggagt aggcggtgcc ctctatggct
     1141 ttaccgggat cggcggccag cgaaccgctt tcgatgagca actgcgcgcg gctgtgcaat
     1201 tggttgtcga tgtcggcgta gagcgccctg gagaccacga catagacggc gagcgccatc
     1261 agcacgacga ccatcgcgac catcgacatg gcgagcagca tcacccgcca gcgcagtgac
     1321 agcgagctgg gttcgggaat cgaactgggt cgcaagtgag gtggtcgaaa cgtggccatt
     1381 actaccgtcg catcttcgcc caagcgtccg ccgcaagcgg ggcgggcccc agcttgcgag
     1441 gcgagtaccc ggacatcacg gcggtgtctc acgcagcacg taacccaccc cgcgcacggt
     1501 gtggatcagc cgcggctcac cttccgcttc cgtcttgcgg cgcagataac cgacgtacac
     1561 ctcgagggcg ttgcctgagg tcg
//
LOCUS       DASYLX010000640         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3543079,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000640 DASYLX010000000
VERSION     DASYLX010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3543079"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1016
                     /locus_tag="VGP27_15130"
     CDS             153..1016
                     /locus_tag="VGP27_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852665.1"
                     /translation="MSVFIDIAPAESHVGSPATAAPTAKWRGRLARAALPVLSLIVFF
                     VVWQVAAASGIWNQTFVPYPGTVWRAFIAVSTTHDGVRGYAGYLLWEHLYMTLRRVLA
                     GVVIGVLLGVLLGLVMGSVSWVRSVLEPWLTFLRALPPLAYFFLLVIWLGIDEAPKVT
                     LLALAALPPAAVATTAAVVAAPVGLQEAARALGASRRQTIRDVVVPSALPETFTGIRL
                     AVGMAYSSVVAAELFNGIPGIGGLVKDASNYNNTPVVLVGIFAIGISGLVIDGLLRAI
                     ERRAVPWRGKI"
     gene            1017..2075
                     /locus_tag="VGP27_15135"
     CDS             1017..2075
                     /locus_tag="VGP27_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7852666.1"
                     /translation="MNFIRAARAATFRRALLAALAVTTLALAGCSVDQPGQQADKPTI
                     RVGYQSFPSGDLVVKNNKWLEKALPDYNIKWIKFDSGADVNTAFIAKELDFGALGSSP
                     VARGLSEPLNIPYKVAFVLDVAGDNEALVARNGTGIDSIAQLRGKRIATPFASTAHYS
                     LLAALSQNKLSLSDVALVDLQPQAILAAWERGDIDAAYTWLPTLDDLRKGGKDLITSR
                     QLARDGKPTLDLAAVADDFASAHPEVVDIWRQQEARALTTIKNDPNAAATAIAAEIGL
                     SPQETAGQLKQGVYLTPEQVASPEWLGSDGKPGNIAVNLQSASQFLADQKQIPAAAPL
                     KTFQDAVYTKGLPGVLSQ"
     gene            2059..2868
                     /locus_tag="VGP27_15140"
     CDS             2059..2868
                     /locus_tag="VGP27_15140"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7852667.1"
                     /translation="MSSVSDLKDATDRRHAGAIRISDVSHRYGRGRAQVTALGPVDLT
                     VDPGSFLVLVGASGCGKSTLLRLLAGFEAPSQGSVQVSGSPPTPGVTTGVVFQQPRLF
                     PWRTVGGNIDLALKYAKVPRERRTERRTQLLERVGLEGTADRKIWEISGGQQQRVAIA
                     RALAAETPLFLLDEPFAALDALTRERLQEDVRQVSAESGRTTVFVTHSADEAAFLGSR
                     IVVLTRRPGQVALDLPVDLPRGDVDADELRRSPEYADLRTEVGRAVKAAAA"
     gene            2965..>3737
                     /locus_tag="VGP27_15145"
     CDS             2965..>3737
                     /locus_tag="VGP27_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEV7852668.1"
                     /translation="MSEITAAPDALRDEPPGTGPTRKLRGNLGVASIVFMVVAAAAPL
                     GVIGGVVPLGIASGNGAGFPATFVVSTVILLFFAVGFTALTPYVEDAGAFFSYVRKSL
                     GFPAGIGIAFVALVSYVAIEAGVYGLLGPAGGVIVELFGGPALPWWVFAAVAFAATTF
                     VGYRNIELSSKVLAVLLTAEIAIVVVLDAVIVFQGGDHGLSNGIVNPSAIFSGSLGIG
                     LLFALISYVGFEATAIFRDEARTPDRTIPRATYVALILIG"
BASE COUNT          588 a         1283 c         1194 g          672 t
ORIGIN      
        1 tttcgcagcc ggcaataaat tctcgctgag cctgaatatc ctccagacaa ctcccagccg
       61 gtaccaatat cgggtgacta ccgcgccctc tgatgactcc tgttgtggct gaaccctctt
      121 cgctttgtct gcgcaacagg aagtcgtgaa cagtgtcggt tttcatcgat atcgcccccg
      181 ccgagtcgca cgtcggctcg ccggcaacgg ccgctcccac agccaagtgg cgcggcaggc
      241 tcgcgcgcgc cgcgctaccg gtgctgtcgc tgatcgtctt cttcgtcgtg tggcaggtgg
      301 ccgcggcgag cggcatctgg aaccagacgt tcgtgcccta cccgggcacg gtgtggcgcg
      361 cgttcatcgc cgtctccacg acacacgacg gcgtccgcgg ctatgcgggc tacctgctgt
      421 gggagcacct gtacatgacg ttgcgccgcg tgctggcggg cgtggtcatc ggcgtgctgc
      481 tcggcgtgct gctcggcctc gtcatgggct cggtcagctg ggtgcgcagc gtgctcgaac
      541 cgtggctgac gtttctgcgc gcactgccgc cgctggccta cttcttcctg cttgtcatct
      601 ggctcggcat cgacgaggcg ccgaaggtca cgctgctcgc gctggccgcg ctgccgcccg
      661 ccgcggtcgc gaccaccgca gccgtcgtcg ccgcacccgt cggcctgcag gaggccgccc
      721 gtgccctcgg cgcgtcgcgc cgccagacca tccgagacgt cgtcgttccg tcggcgcttc
      781 cagaaacgtt caccggcatc cggctcgcgg tcggcatggc ttactcctcg gtggtcgccg
      841 ccgaactgtt caacggcatc cccggtatcg gcggcctggt caaggacgcc agcaactaca
      901 acaacacccc cgtcgttctg gtcggcatct tcgccatcgg gatctccggc ctggtgatcg
      961 acggtctact tcgcgccatc gaacggcgtg ctgtcccctg gagaggaaaa atctagatga
     1021 atttcatccg agccgctcgg gcggcgacat tcagacgcgc ccttctcgcg gcactggcag
     1081 tgaccacgtt ggcgttggcc ggctgctcgg tggaccaacc cggtcagcag gccgacaagc
     1141 cgacgattcg cgttggttac caaagctttc ccagcggcga cctcgtcgtg aagaacaaca
     1201 agtggctgga gaaggcgctg ccggattaca acatcaagtg gatcaagttc gactccggcg
     1261 cggacgtcaa caccgcattc atcgccaagg aactcgactt cggcgcgctg ggttccagcc
     1321 cggtggcccg cggactgtcg gaaccgctga acatccccta caaggtcgcg ttcgtgctcg
     1381 acgtggccgg cgacaacgag gcgttggtgg cccgcaacgg caccggtatc gactccatcg
     1441 cacagctgcg cggtaagcgc atcgccacac cgttcgcgtc gacggcgcac tacagccttc
     1501 tcgctgcgct gtcgcagaac aagctgtcgc tcagcgatgt tgcgctcgtc gaccttcagc
     1561 ctcaggccat tctcgcggcg tgggaacgag gcgacatcga cgccgcctac acctggttgc
     1621 cgacgctgga cgatctgcgt aagggcggca aggatctgat caccagtcgc cagctggcca
     1681 gagacggcaa gccgacgctg gacctggccg cggtcgccga cgatttcgcg tccgctcatc
     1741 ctgaggtcgt cgacatctgg cgtcagcagg aggcccgcgc gctgacgacg atcaaaaatg
     1801 accccaacgc cgccgccacg gccatcgcgg ccgagatcgg cttgtcgccg caagagaccg
     1861 ctggacaact caagcagggc gtttacctga caccggaaca ggtcgcatcg ccggaatggc
     1921 ttggctccga tggcaagccg ggcaacatcg cggtcaatct gcagagcgcc tcgcagttcc
     1981 tcgccgacca gaagcagatt ccggcggcgg cgccgttgaa gaccttccag gacgcggtct
     2041 acacgaaggg actgccgggt gtcctcagtc agtgacctca aggacgcgac cgatcgccgg
     2101 catgcggggg cgattcgcat cagcgatgtg tcgcatcggt acggccgcgg gcgcgcccag
     2161 gtcaccgcgc tcggaccggt cgacctgacc gtcgacccgg gttcgttcct ggtactcgtc
     2221 ggcgcatcag gatgcggcaa gagcacgttg cttcggttac tcgccggctt cgaggcgccc
     2281 agccagggct ccgttcaggt gtcgggcagc cctccgacac cgggcgtgac cacgggtgtg
     2341 gtgttccaac aaccgcggct tttcccgtgg cggacggtcg gcggcaacat cgacctggcc
     2401 ctcaaatacg ccaaggtgcc tcgggagcgc cgcactgaac gccgcacgca actgctcgag
     2461 cgggtgggcc tggaaggcac tgccgaccgc aagatctggg agatcagcgg cggccagcaa
     2521 cagcgcgtcg cgatcgcacg ggcgttggcc gccgagacgc cgctgttcct gctcgacgaa
     2581 cctttcgcgg cgctcgacgc cttgacgcgg gaacggttgc aggaggacgt ccgtcaagtc
     2641 agcgccgaat ccggacgcac cacggtgttc gtcacacaca gcgccgacga ggcggcgttc
     2701 ctcggatcgc gcatcgtggt gctgacacgt cgacccggcc aggtggcgct tgatcttccg
     2761 gtggatctgc cgcgcggcga cgtcgacgcg gacgaactgc gccgctcacc ggaatacgcg
     2821 gacctgcgta ccgaagtggg ccgtgccgtc aaggccgccg cagcctagta agactcatga
     2881 tgactccaga tgtggtcctg caccgtcatt cagtcctacg gttccgtcaa cccatcgact
     2941 cccccatcga aggatggtcc ccgcatgtcc gaaatcaccg ccgcccctga cgctttgaga
     3001 gacgagccgc caggtacagg tccgacacgc aaactgcgcg gcaacctcgg cgtggcctcc
     3061 atcgtcttca tggtggtggc cgccgccgcg ccgctcggcg tgatcggcgg cgtcgtgcca
     3121 ctgggcatcg catcgggcaa cggcgccggg ttcccggcca cgttcgtggt ctccacggtc
     3181 atcctgctgt tcttcgcggt cggattcacc gcgctcacac cgtatgtgga ggacgcgggg
     3241 gcgttcttct cctacgtccg caagtcgctg ggcttcccgg ccggtatcgg tatcgcgttc
     3301 gtcgcgctcg tcagctacgt agcgatcgag gcaggcgtct acggcctgct gggtccggcg
     3361 ggcggcgtca tcgtcgaact gttcggcggc ccggctctgc cctggtgggt gttcgcggcg
     3421 gtggcattcg ccgccacgac gttcgtcggc taccgcaaca tcgagctgtc cagcaaggtg
     3481 ctggccgtgc tgctgaccgc cgagatcgcg atcgtggtgg tgctggatgc ggtcatcgtc
     3541 ttccagggcg gtgaccacgg gttgtccaac ggcatcgtca acccgagcgc catcttctcc
     3601 ggctcgctgg gtatcgggct gctgttcgct ttgatcagct acgtcggttt cgaggccacc
     3661 gcgatcttcc gagacgaggc ccgcacgccg gaccgcacca ttccgcgcgc cacctacgtc
     3721 gcgctgatcc tgatcgg
//
LOCUS       DASYLX010000641         7972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_581969,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000641 DASYLX010000000
VERSION     DASYLX010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581969"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGP27_15150"
     CDS             <1..662
                     /locus_tag="VGP27_15150"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7852669.1"
                     /translation="PEGFDYDKHFVPSYKPWDQRVCLAPNGDIFKEIRKGHADVVTDT
                     IERFTKTGIKLASGEELQADIIVTATGLNMQLLGGLEPTKNGQTIDLTSLMTYKGLMF
                     SGIPNFAMTFGYTNSSWTLKADLVSEFVCRLLNYMDDNGFDRVEPQHPGDSVDELPFM
                     DFSPGYFKRSEHLLPKSGSRAPWRLKQNYLFDMRMIRHGKVDEEALHFTKHRVAVPAS
                     A"
     gene            complement(666..1148)
                     /gene="rraA"
                     /locus_tag="VGP27_15155"
     CDS             complement(666..1148)
                     /gene="rraA"
                     /locus_tag="VGP27_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEV7852670.1"
                     /translation="MSIEPRPTADLVDDIGSDVRSCDLQLHQFGGRSEFAGHITTVKC
                     FEDNALLKSVLSEPGDGGVLVIDGDGSLHSALVGDIIAGLGVDNGWAGLIINGAVRDA
                     ATLNTLDIGIKALGTNPRKSAKTGDGQRDVAVEFGGVVFVPGEIAYSDDDGIVVVTAS
                     "
     gene            complement(1169..1702)
                     /locus_tag="VGP27_15160"
     CDS             complement(1169..1702)
                     /locus_tag="VGP27_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852671.1"
                     /translation="MADQQDRPDEKPEAPPPTPPTPPQFSDAASRATPPPVEPPAPPP
                     PPPAKKAPAKQAKAAKKAPAKTPKKTPAKAAKKAPPAKKAAKKAAPGKATPPAPPRVA
                     PKLADTNGDRNLADAAKEAAAQAKSTVAAAGNPVNGPGTVPLVGPEPSRVPLAAAIAA
                     GVLAILLVLVARRHGDD"
     gene            complement(1745..2668)
                     /locus_tag="VGP27_15165"
     CDS             complement(1745..2668)
                     /locus_tag="VGP27_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEV7852672.1"
                     /translation="MTRWRAVIGGVIVVLGASAAASALAFPQAAPAATVVRALADCAT
                     VVTLGLAVVPMLDTGRYRDELTRRAAGPLAWAAGFWLFAELCRLVVGAAAAAGVPVMR
                     VGLTTTAEFAYATSAGRSGLFSAAAAGLVMIVALVAAKTPPAAVAAAGITAVGLVART
                     IGGHMSASTFGGIAIAIHVLAAAAWCGTLAALVLTIDHRGQWARVLPRFSELSLLSVI
                     SLLASGLTGALAALDSASQLFTTGYGSLLLTKIVLTVVLMTLAWHNRARWLPAAKSHR
                     VTAYVSRSRSLTELAIMAVALTMAAALSVTG"
     gene            complement(2665..3126)
                     /locus_tag="VGP27_15170"
     CDS             complement(2665..3126)
                     /locus_tag="VGP27_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEV7852673.1"
                     /translation="MALAGAPVASAHAVRVETDPADNAALAQGPARVSATFNEQLQPQ
                     FAAMTVVGPDGNLWSDGDAQVQGAVISVGVRRLGPAGAYTVNYRVTSADGHPVSGSWS
                     FRLTVAAGGTPGPSAAKPADTDGGGIPVWPFLIAGALIIGGGILWAARRKT"
     gene            complement(3171..3839)
                     /locus_tag="VGP27_15175"
     CDS             complement(3171..3839)
                     /locus_tag="VGP27_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HEV7852674.1"
                     /translation="MRFSPRARKRALITVACTGVALSIGLASGAGPASAHVHADADNP
                     APGATSMVTFQVPGESENGALTTKLSVKLPDVASARTEVMPGWQASLDRDTAAGTVRS
                     VTWTADPKVGISSDQFALFRVSVKLPEGDSVTFPATQTYSDGTVVQWDQPPLPGGGEP
                     EHPAPTVSLTGASATGVEDHEATAQAAPTAAKADTAARWLAGGALVLAAVGVAAAVIS
                     RRRT"
     gene            complement(3876..4403)
                     /locus_tag="VGP27_15180"
     CDS             complement(3876..4403)
                     /locus_tag="VGP27_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852675.1"
                     /translation="MTAQSSATAARLRGTAAGLLTAALAVAAHGVADGAAPSGSAVVL
                     LAVLGATVGGLAATISRSADVRVLLALLASGQIVGHLMLSAAGHHHGEPTSPAPSMLV
                     AHALAVVAGAVLIAAGDRLCRAVSRVVEVAMRTVLPPIPSRPVLAASADQPLRSSLLL
                     AASMSHRGPPVSRAR"
     gene            complement(4411..5067)
                     /locus_tag="VGP27_15185"
     CDS             complement(4411..5067)
                     /locus_tag="VGP27_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HEV7852676.1"
                     /translation="MGLFKRRKSRATRRAEASAIKAKAKLEAKLAAKNENRRMKAAHK
                     AETRALKAQLRAQRQSDRTALKVAETELKAVRDGKLLSPARIRRSLTVARLLAPIVVP
                     LAYRAAMAARGLLDQRRADRLGVPLSQLGQFSGHGAGLSARVAGAEQTLRQVAEKKPK
                     DPETKQFVTAMTDRLTDLSAAVTAAENMPTANRRSAHDSISQQLDGIEADLMARLGVA
                     "
     gene            5279..6235
                     /locus_tag="VGP27_15190"
     CDS             5279..6235
                     /locus_tag="VGP27_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852677.1"
                     /translation="MASRLRVAASTCLVASGLFMGGAGSAIALADPEPGYGPSDDRTG
                     GESLGQTSRQALGTAGGNDRTPSGEFRRQPQPRWGNGRPNEKDPPKGEQPEPGKENPQ
                     DPKGDEQPPGEDPKDPCGDDAGNCPGDGTGDRPPNLPERGGGGGAIVQLPGFEPPKVP
                     HMQLPGELEPSQPGTRGGPAVLDAGAGAVAVAAPAGPIVLPVIVVPPVGLGAGAGGGG
                     AGGASAGLPPAAPRAVTAEPPAGRVAQPAKVGSNVAVPNSSYRIGYTEYLRTAGLPQV
                     AALAVPGLVGILVLTGAGGLVGFRQAKAGHAVRVGGSARFMR"
     gene            6303..6794
                     /locus_tag="VGP27_15195"
     CDS             6303..6794
                     /locus_tag="VGP27_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF955 domain-containing protein"
                     /protein_id="HEV7852678.1"
                     /translation="MPASRRVTRAVSAVLDLAPRRGEISLPRLVDAVSEDRKRPIELK
                     MSDLPPGVCGQWRQYADRDVFLIQKGLPAWDRTLAHELGHLVLGHEGIPVVQAAREET
                     EFASSELIGYMLNQRTGCMGPSGEDAEQEAEDFAALLIYRLGRLPSDRSSIVQVRLGE
                     AFG"
     gene            6791..7966
                     /locus_tag="VGP27_15200"
     CDS             6791..7966
                     /locus_tag="VGP27_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852679.1"
                     /translation="MIIWVIAGLLGLATGLRIGWALVNKQSLVSTAMILALGSLGLVA
                     ALNWQPLTLLLDTMLHWPNVSMGLSQVALVACAAGSCVMITTVSSPRKPAILRRIALC
                     QYGVAVVIAVATLVTFFVAGQQSEMSPEEYLRRNLGSNGTALSWAWLLPLLYVLLALT
                     LVSWAGMRHSNRTRRGRALFVFTVGIVLIVLASAFFFLRAVGTTQFVGVGAAATLLGC
                     AMLVVAAGSLLPSVEDWFGARRELRIIQPMLDELGRRHPDVGIGVRPRGPLVFRVAER
                     MSLISDALFLEATTADAALRDRVEASCGSSDRDLEQEIAEIADAETPDVKPEEQAHAI
                     AEWIYAGRGETDEDTKRAWFPGLGWLRQPRAYSDREWILAIAKQYRELDRAGVAAAS"
BASE COUNT         1273 a         2688 c         2712 g         1299 t
ORIGIN      
        1 tgccggaagg cttcgactac gacaagcact tcgtgccgag ctacaaaccg tgggaccagc
       61 gcgtgtgtct tgcgccgaac ggcgacatct tcaaggagat ccgcaagggt cacgccgacg
      121 tcgtcaccga caccatcgaa cggttcacca agacgggcat caagctggca tccggcgagg
      181 aactgcaggc cgacatcatc gtcaccgcaa ccggtttgaa catgcagctg ctcggcggcc
      241 tcgagccgac caagaacggc cagaccatcg acctgacgtc actgatgacc tacaagggcc
      301 tgatgttctc cggcatcccg aacttcgcca tgacgttcgg gtacaccaat tcgtcgtgga
      361 cgctgaaggc ggatctggtc tccgagttcg tctgccggct gctgaactac atggacgaca
      421 acgggttcga ccgtgtcgag ccgcagcacc ccggcgacag tgtcgacgag ctcccgttca
      481 tggacttctc cccaggctac ttcaaacggt cggagcacct gctgccgaag tccggttcac
      541 gggcaccgtg gcgactcaag cagaattacc ttttcgacat gcggatgatc cgccacggca
      601 aggtcgacga agaggcgctg catttcacca aacaccgtgt cgcggtgccg gcttcggcct
      661 aatcgtcagc ttgcggtgac gacgacgatc ccgtcgtcgt cgctgtaggc gatctcaccc
      721 ggcacgaaga ccaccccacc gaactcgacc gccacatcgc gttggccgtc gccggttttc
      781 gcgctcttgc ggggattcgt gccgagcgcc ttgataccga tatccagtgt gttcagcgtg
      841 gcggcgtcac ggaccgcgcc attgatgatc aggcccgccc agccgttgtc gacgccgagg
      901 cccgcgatga tgtcgccgac gagagccgag tgcagcgacc cgtcgccgtc gatcaccagc
      961 acgccgccgt ccccgggttc cgaaagcacc gacttcagca acgcgttgtc ttcgaagcac
     1021 ttcaccgtgg tgatgtggcc cgcgaattcg gaccggccgc cgaactggtg caactgcaga
     1081 tcgcagctgc ggacgtcgga gccgatgtcg tcgacgaggt cggcggttgg acggggttcg
     1141 atgctcacgg gtcgatgcaa gtaattcgtc agtcgtcacc gtgccggcga gcgaccaaca
     1201 ccagcaggat ggccagcacg cctgcagcga tcgccgccgc gagcggcaca cgcgacggtt
     1261 cgggtcccac cagcggtaca gtgccagggc cgttcacggg gtttcccgcc gcggctacgg
     1321 tcgatttcgc ttgtgctgca gcctctttcg ccgcatctgc gagattacga tcaccattgg
     1381 tgtcggccag cttgggtgcg actctcggcg gagcaggggg cgtcgccttc cccggggcgg
     1441 cctttttcgc cgccttcttg gcgggcgggg ccttcttggc cgccttcgca ggtgtcttct
     1501 tgggtgtctt cgccggtgcc ttcttggcgg ccttggcttg tttggcgggc gctttcttgg
     1561 ccggaggagg tggaggcggt gcgggcggct caacaggcgg cggggtggcc cgcgacgcgg
     1621 cgtctgagaa ttgcggcggc gtcggcggcg tcggcggcgg agcctcgggt ttctcgtccg
     1681 gtcgatcctg ctggtcggcc atcaggctgc tcctttgcag tgtctcgtcc tcagccattc
     1741 aaccctaacc ggtgactgac agcgctgcgg ccatggtcaa cgccaccgcc atgattgcca
     1801 gttcggtgag tgacctggat cgtgacacgt aggcggtgac ccggtgcgac tttgccgcag
     1861 gcagccagcg ggcgcggttg tgccaagcca gcgtcatcaa cacaactgtc agaacgatct
     1921 tggtcagcag caagctgccg tagccggtgg tgaacaactg cgatgccgag tccagtgccg
     1981 cgagagcgcc tgtcaaacca ctggccagca acgagatcac ggacaacaac gacagttcgg
     2041 agaagcgggg cagcacgcgc gcccactgcc cacggtgatc gatcgtcaac accagcgccg
     2101 ccagcgtccc gcaccacgcc gcggccgcca gaacgtgaat cgcgatagcg ataccgccga
     2161 acgtgctggc cgacatgtgg ccaccgatcg tgcgcgcgac cagaccgacc gccgtgatcc
     2221 cggcggcggc gaccgctgcg ggcggcgtct tggccgcgac cagagcaacg atcatcacaa
     2281 gtccggcggc agcggcactg aacaggccgg atcgcccggc ggacgtcgcg tacgcgaact
     2341 ccgccgtcgt ggtcaggccg acccgcatca ccggcacccc ggcggccgcg gcggcaccca
     2401 cgacgagcct gcacagttcg gcgaacaacc agaagcccgc cgcccacgcc aggggcccgg
     2461 cagcccgccg cgtcagctcg tcccgatacc ggccggtgtc gagcatcggc accaccgcaa
     2521 ggccgagcgt gacgacggtc gcacagtcgg cgagtgcgcg caccacggtc gcggcgggcg
     2581 cagcttgtgg aaaggccaac gccgacgccg ccgccgacgc gccgagcacg acgatgacac
     2641 cgccgatcac cgcccgccac cgcgtcacgt ctttcgtcgt gccgcccata ggattccgcc
     2701 gccgatgatc aacgcgcccg caatcaggaa cggccacacc ggaattccgc cgccgtcggt
     2761 atcggcaggc ttggctgccg aggggcccgg cgtgcctcca gcggccaccg tgagccggaa
     2821 ggaccacgaa cccgacactg gatggccgtc cgcagacgtc acccggtaat tcactgtgta
     2881 ggcgcctgca ggcccgagcc gccgcacccc gacggagatg accgcgccct gcacctgcgc
     2941 atcgccgtcg gaccacagat ttccgtcggg acccacgacg gtcatcgcgg cgaactgcgg
     3001 ctgcaactgt tcgttgaacg ttgcgctgac ccgggccggc ccctgggcaa gcgcggcgtt
     3061 gtcggcggga tcggtctcga cccggacggc gtgcgcggaa gccacgggcg cgccggccag
     3121 cgccatcacc gccaccagga cggcgatgaa cactagccgg actgaccggg tcaagtgcgc
     3181 ctccgagaga tcaccgccgc ggcaactccc accgcggcca acacgagtgc gccgcccgcg
     3241 agccaacgtg cggcggtgtc ggccttggcc gccgtcggcg cagcctgcgc cgtcgcctca
     3301 tggtcctcca ctcccgtcgc cgacgcaccc gtcaagctca ccgtgggtgc cgggtgttca
     3361 ggctcaccgc cgccgggcag cggcggctgg tcccactgca ccaccgtgcc gtcggaatac
     3421 gtctgagtgg cggggaaggt gacgctgtca ccctcgggca gcttcaccga cacccggaac
     3481 agtgcgaact ggtccgacga aattcccacc ttggggtcgg ccgtccaggt gaccgagcgg
     3541 accgtgccgg ccgcggtatc gcggtcgagg ctggcctgcc agccgggcat gacctcggtt
     3601 cgcgcggacg ccacgtcggg cagtttgacg ctcaatttgg ttgtcagcgc gccgttttcg
     3661 gattcaccgg gcacctggaa cgtcaccatt gaagtggcgc cgggtgcggg attgtcggca
     3721 tcggcatgga cgtgcgccga tgctggccct gcgccggacg ccagaccgat cgacagtgct
     3781 acccctgtgc aggcaacggt gatgagggcg cgcttacgcg cccttggtga aaaacgcatt
     3841 cgattgtcac tttcttgttg tcgaggagcg ggcggtcagc gtgcgcggct gaccggcgga
     3901 cctcgatgtg acatcgatgc ggcgaggaga agggacgacc gcagaggctg atcggcgctc
     3961 gcggcgagga cgggccggct cggtatcggc ggaaggacgg tgcgcatggc cacttcaacc
     4021 acgcgcgaca ccgcgcggca gagccggtcg cccgcggcga tcaggaccgc tccggcgacc
     4081 accgccaggg cgtgcgctac cagcatcgac ggagcgggcg acgtcggttc gccatggtgg
     4141 tggcccgcgg cgctgagcat cagatggccg acgatctggc cgctcgccag cagtgcaagc
     4201 agcacgcgca cgtcggcgct ccgcgagatc gtcgccgcca ggcctccgac cgtcgcgccg
     4261 agcacggcca gcagtacgac ggccgagcct gacggcgcag cgccgtcggc gacaccgtgg
     4321 gcggccaccg ccagcgccgc ggtcagcagc ccggcagccg taccgcgcag acgggctgcg
     4381 gtggcgctcg actgcgctgt cacgggctgg tcaggctacg ccgaggcgcg ccatcaggtc
     4441 ggcttcgatg ccgtcgagct gctggctgat ggagtcgtgg gcggatcgcc ggttggccgt
     4501 cggcatgttc tccgccgcgg tcactgccgc cgacaggtcg gtcagtcgat ccgtcatggc
     4561 ggtgacgaat tgctttgttt cagggtcttt gggcttcttc tcggccacct gtcgcagtgt
     4621 ctgctcggct cccgcaacgc gcgccgacag cccggcgccg tgcccggaga actggccgag
     4681 ctggctgagc gggacgccca gcctgtccgc cctgcgctga tcgagtagtc cgcgggcggc
     4741 catcgcagca cggtaggcga gcggcaccac gatcggagcc agcagccggg ccaccgtgag
     4801 cgaccggcgg atgcgcgccg gcgacagcag cttgccgtcg cggaccgcct tcagctcagt
     4861 ttcagcaacc ttgagcgcgg tccggtcgct ttgtcgttgc gcccgcagtt gtgccttcag
     4921 cgccctggtc tcggccttgt gggcggcctt catgcgacgg ttctcgttct tcgccgcgag
     4981 cttggcctcc agcttcgcct tggccttgat ggcactggcc tcagctcgcc gagtagcgcg
     5041 gctttttcgt cgcttgaaca ggcccatccc ggccgccttc ccggtgatct cgtcggcatt
     5101 tcggccattc gtgtcagcga tggcgcagca caacactatc gctcgcgcga tgaccggcgc
     5161 ttgcgcgccc cgaaatgcga ctggttccag ccactagcaa acgcgaacga gggattgtgc
     5221 gacaatcgtc gcaaccgtca acggttgttc gggaggcgtc acgagaggtg gtggccgggt
     5281 ggcttcgcgt ctgcgtgtcg ccgcgtcgac atgcctcgta gcgtctggcc ttttcatggg
     5341 cggtgccggc agcgcaatcg ctcttgctga ccccgaaccc ggctacggcc cctctgacga
     5401 ccgcacgggc ggcgaatcgc tgggccagac cagtcgtcaa gcgctgggca cggccggtgg
     5461 caacgaccgg acgccgtcgg gtgaattccg caggcagcca caaccgcgct ggggaaacgg
     5521 ccggcccaat gagaaggatc cgccgaaagg cgaacagccg gagccgggca aggaaaatcc
     5581 ccaagatccg aagggcgacg agcagccgcc aggtgaggac ccgaaggacc cctgtggcga
     5641 cgacgcaggc aactgcccgg gcgacggaac gggcgaccga ccgcccaacc tccctgaaag
     5701 gggcggcgga ggcggcgcga tcgtccagct gccgggcttc gagccgccga aggtccccca
     5761 catgcaacta cctggcgaac tagagccgag ccagcctgga acgcgcgggg ggcccgccgt
     5821 gctcgacgca ggcgcaggcg cggtcgctgt ggcagcgcct gcggggccca tcgtcttgcc
     5881 ggtgattgtc gtgccgcccg tcggtctcgg tgcaggcgcc ggtggcggag gagccggagg
     5941 cgcatcggcg ggactgccac ccgcggcgcc gcgcgccgtg accgccgagc cgcccgccgg
     6001 acgcgtcgcg cagccggcga aggtgggcag caatgtcgca gtaccgaatt cgtcctaccg
     6061 gatcgggtac accgaatacc tgcggaccgc tggattaccg caggtggcag ccttggcagt
     6121 gccagggctg gtcggaattc tggtgctcac gggcgcaggg ggattggtgg gtttccggca
     6181 agcaaaagcg gggcacgccg tgcgagtggg cggctcagca cgtttcatga ggtagtacta
     6241 caaactgccc ccctgaacag gggcgggcag cgcatagtgg actcgtcgag agggggatgg
     6301 ttatgcctgc gagtcgtcgc gtcacccgtg ccgtcagcgc ggtgctcgat ctcgctccgc
     6361 gacgcggaga gatctcgttg ccccgccttg tcgatgcggt cagcgaggac cgcaagcggc
     6421 cgatcgaact caagatgtcg gatctgcctc ccggcgtgtg cggccaatgg cggcagtacg
     6481 ccgatcgaga cgtgttcctg atccagaagg gcctgcccgc ctgggaccga acgttggcgc
     6541 acgagctcgg ccaccttgta ctcggccacg agggcattcc cgtcgtccaa gccgcccgcg
     6601 aggagacgga gttcgccagc tcagagctga tcggatacat gctcaaccag cgcaccggct
     6661 gcatggggcc gagcggtgaa gacgccgagc aggaggccga ggatttcgcg gcgctgctca
     6721 tctaccgcct gggcaggctg ccctccgacc gctcctcgat cgtgcaggtt cggctcggag
     6781 aggcgtttgg ttgatcatct gggtgatcgc aggtcttctc ggacttgcca ccggcctgcg
     6841 tatcggttgg gccctcgtca acaagcagtc gctggtgagc accgccatga ttctggcgct
     6901 cggcagcctg ggattggtgg cggctctcaa ttggcaaccg ctgacgctgc tgctggacac
     6961 aatgttgcac tggccgaacg tctcgatggg tctgagccag gtcgctctgg tcgcctgcgc
     7021 cgcgggcagc tgcgtgatga tcacgacggt ctcgtcgcca cgtaaacccg ccattcttcg
     7081 gcgcatcgcg ctctgccagt acggtgtcgc cgtcgtcatc gccgtcgcca ccctggtgac
     7141 gttcttcgtt gcgggccagc agtcggagat gtcgcccgag gagtacctgc ggcgcaacct
     7201 cggttcgaac ggaacggcgt tgtcgtgggc gtggctgctg ccgctgcttt acgtgcttct
     7261 ggcgctgacg ctggtgtcgt gggccggtat gcgccattcg aatcgcaccc gccgaggcag
     7321 ggcgttgttc gtgttcaccg tcggcatcgt gctcatcgtg ctcgcaagcg ccttcttctt
     7381 cctgcgggcg gtgggcacca cgcagttcgt cggagtcggt gcggccgcaa cgcttttggg
     7441 ttgcgccatg ctggtggtgg cggccggctc gctgcttccc agcgtcgagg actggttcgg
     7501 tgcgcgtcgc gaactgcgga tcatccaacc gatgctcgac gaactcggcc gccgacatcc
     7561 cgacgtcgga atcggggtac ggccgcgtgg cccgctggtt ttccgggtgg ccgagcggat
     7621 gtcactgatc tccgacgcac tgttcctgga agcgacgacc gcggacgccg cgctgcgtga
     7681 tcgggttgaa gcctcctgcg gatcgtctga tcgcgacctg gagcaggaaa tcgccgagat
     7741 cgccgacgcc gagacgcccg acgtcaagcc tgaggaacag gcacacgcga ttgcggagtg
     7801 gatttacgcc ggccgcggcg agaccgacga ggacaccaag cgagcatggt tccccggtct
     7861 gggttggctg cgtcagccga gggcctactc ggaccgggag tggatcctcg ccatcgcgaa
     7921 gcaataccgg gagctggaca gggcgggtgt cgcagccgcc tcctgatgga gg
//
LOCUS       DASYLX010000642         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1020047,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000642 DASYLX010000000
VERSION     DASYLX010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020047"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGP27_15205"
     CDS             complement(<1..269)
                     /locus_tag="VGP27_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7852680.1"
                     /translation="MTLPRSLARGAAVLSAVGAAATAVIVAPTATAAPCPDIEVVFAR
                     GTAEPAGIGRVGQSFTDALSTQVGGRTVGSYAVNYPAGYDFLTTA"
     gene            complement(303..1313)
                     /locus_tag="VGP27_15210"
     CDS             complement(303..1313)
                     /locus_tag="VGP27_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7852681.1"
                     /translation="MTATQLTRGADHFRLGLLFAVGSALSFGMSGPLAKALMDAGWSP
                     TGAVTARMAVGALAMAVFATIVKRDWVREAVAHRKIVIAYGIFPIAGAQLCYYNAVAH
                     LSVGVALLLEYTAPVLVVGWLWVTTRRRPTNLTLAGVALAVAGIMLVLNVFAGAHINA
                     AGVAWGLAAAVCAASYFMMSDEVAADGTGLNSITLAAGGLVVGAAATTALGVLGVMPM
                     TFATDDTVVAGLTMSWLVPVIALGVITTAIAYTLGIMGIARLRPRFASLLGLSEVMFA
                     VVAAWLLVGEAMTLTQAVGGAVVLGGLALARQGDRGEVAAATWPDAGAVGDLTPCNRA
                     NS"
     gene            1388..1942
                     /locus_tag="VGP27_15215"
     CDS             1388..1942
                     /locus_tag="VGP27_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV7852682.1"
                     /translation="MNFTYDTELTLAAARVLINSERVNGEQLGDLEALDAYLDSFGWT
                     GRRDHDSAELESVHRLRGRLGTIWGAAHDEDTAVAHVNALLSDTKAQPWLTRHPEMPE
                     WHLHLASVHDPLWQRMGAEIAMALADLIRGGELRRLKICAASDCEAVLVDMSRNRSGK
                     FCDTGNCGNRQHVAAYRERRAQES"
     gene            complement(1947..2831)
                     /locus_tag="VGP27_15220"
     CDS             complement(1947..2831)
                     /locus_tag="VGP27_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852683.1"
                     /translation="MTNPMNYNQQLLNYLQAWRQLLEASAALTSGMPMGPSPMGMPPM
                     PSMPPMPFMPPMTAPGTVPPGASPPADYSQQLFGYLQAWRQYLEHATSNASGSIQPLQ
                     PMGSYPGSYPNGYPAAEQSAGSQSSASQSTDSSSTGSQATGDTGSEASGTQSSGSQTS
                     FAPKNENRVVLRPKDPYGTISGFNLSGRGEQPNRAEFTGGSSDPGVRSAFARKLAPEA
                     TTSPVHGASLFGRAATTPSPTVSTTVTGAGMDPRHAAPPATSRWWLAGQGQRPGIKEQ
                     RDPTRLVNIRPTDLGGRQ"
     gene            complement(2831..>4600)
                     /locus_tag="VGP27_15225"
     CDS             complement(2831..>4600)
                     /locus_tag="VGP27_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7852684.1"
                     /translation="TIMDNLRRVVTQGTTLEGKPSLVFPREAPRSPAEFLTTASQLTQ
                     LGFTPLDVQTFVSRLIDFLVTSPKRRASELQNLSAYDYFIGRAAPGEPPRYSYSQQFN
                     AVLLEMPRVLAAFDTRWGDARTNVSTYLQLQLQMDRRDNKADGVLNGPTTEAWFDHWY
                     RHLVELGVSFVHGEAANIEASAFDPLQPAHLRPRAQVTFADGTRVAPDYTVVAVDAPV
                     AERITASLRAAGIGGTAAGLDGFTTLVPPPDGPLQPDSTRPVDRRDPYAMDSMGLKAW
                     DRFQTLGGIQYYFDTEFQLLRGHMYYSGTAWALSSINQHGLWEKKPILNRDGHVSVLS
                     VDIGDFNTPSSGNGKAARHCTADEIATEVWHQIVKALTSTSPNVAESLLPWPAWYAID
                     RGIMMEGGPGQGSGVPIRNETPYLVPIVGDWTNRPCAEPWNPTGNSWSTVPGEDDWLE
                     DLQQRNVWQARHGGYQVHNNSVVFAGTWTKTFTRMTSMEAACESGRHAVNAILDHYIW
                     VESGGTDRRGETALDWRTPYGFIDQGLTTPVRMPSPAGDYCYVMDLENREPLDTRTLR
                     NLDAKYQDGGTPVPLQPEWPEDH"
BASE COUNT          790 a         1441 c         1586 g          783 t
ORIGIN      
        1 tctgcagtgg tgaggaagtc atagcccgcc ggatagttca ccgcgtagga accgacggta
       61 cgaccgccga cctgtgtact caacgcgtcg gtgaaggact gaccgaccct gccgatgcct
      121 gcgggttcgg cggtgccgcg cgcgaacaca acctcgatgt ccgggcaggg cgcggccgtc
      181 gcagtcggcg cgacgatcac ggcggtggcg gcggcgccga cggcggaaag gacggcggcc
      241 ccccgggcca agctacgtgg caaagtcacc cggccatatt tccacacccg ggcaacggga
      301 acctaactgt ttgccctgtt gcacggggtc aggtccccga cggcaccggc gtctggccag
      361 gtagcggccg ccacctcgcc acggtcgccc tggcgcgcca gcgccaaccc gcccagtacg
      421 accgcgccgc cgacggcctg cgtcaatgtc atcgcctcgc cgacaagcag ccacgcggcc
      481 accacggcga acatcacctc ggacagcccg agcagggacg cgaaccgggg ccgtagccgg
      541 gcgatgccca tgatgccgag cgtgtaggcg atcgcggtgg tgatcacgcc tagcgcgatg
      601 acgggcacca accacgacat cgtcaacccc gcgacgacgg tgtcatcggt tgcgaacgtc
      661 atcggcatca cgccgagcac gccgagcgcg gtggtcgcgg cggcgccgac gaccaggccg
      721 cccgcggcga gcgtgatgga gttcaggccg gtgccgtcgg cggcgacctc gtcagacatc
      781 atgaaataac tcgccgcgca cacggccgcg gcaagacccc aagccacccc ggccgcgttg
      841 atatgcgcac cggcgaaaac gttgagcacc agcatgattc cggccaccgc cagtgcgacg
      901 cctgccagtg tcaggttggt gggcctgcgt cgcgtggtga cccacagcca acccacgacg
      961 aggacgggcg ctgtgtactc gaggagcagc gcgacgccga cggacaggtg cgcgacggcg
     1021 ttgtagtagc acagctgggc gccggcgatc gggaagatgc cgtaggcgat cacgatcttg
     1081 cggtgtgcga cggcctcgcg aacccagtca cgcttcacga tcgtggcgaa aacggccatc
     1141 gccaacgcgc cgacggccat gcgtgcggtg actgcaccgg tcggactcca cccggcgtcc
     1201 atcagtgcct tcgccagcgg cccggacatg ccgaagctca gggcagagcc gacggcgaac
     1261 agcaggccga gccggaagtg gtcagcgccc ctggtcagct gtgtcgctgt catgtggcac
     1321 ccccggcatg taatgagtaa aatcagcttt ggtaatgacg ctagccggag tggaggtcat
     1381 gagtcaaatg aatttcactt atgacactga gctcacattg gcggcagcac gggtgctgat
     1441 caactccgag cgcgtgaacg gtgagcagct cggcgacctc gaggcgctcg atgcctactt
     1501 ggacagcttc ggctggaccg gtaggcgcga tcatgacagc gccgagcttg agtcggtgca
     1561 ccggttgcgc gggcgactgg gcaccatctg gggggctgcc cacgatgagg acaccgccgt
     1621 cgcccacgtc aacgcgctct tgagcgacac caaggcacag ccctggctga cgcggcaccc
     1681 ggagatgccg gagtggcatc tacatctggc gtcggtgcat gatccgttgt ggcaacgcat
     1741 gggtgcggag atcgcgatgg cgctggccga cctgatccgc ggaggcgaat tgcgccggct
     1801 gaagatctgc gcggcgtccg actgcgaagc agtactggtt gacatgtcgc gcaaccgatc
     1861 cggcaagttc tgcgacaccg gcaattgcgg caaccgccaa catgtcgcgg cttatcgcga
     1921 acggcgtgcg caggagagct gaccggttac tgcctgccgc ccaaatctgt cggccggata
     1981 ttcacgagtc tcgtcgggtc gcgctgctcc ttgatcccgg ggcgctggcc ctggccggcg
     2041 agccaccacc gggacgtggc aggcggggcg gcgtgacggg gatccatgcc ggcccccgta
     2101 acagtcgtcg acactgttgg cgacggcgtg gttgcggcgc gaccaaagag cgacgcgccg
     2161 tgtacgggcg aggtcgttgc ctctggagcc aacttcctgg cgaacgccga gcgaacaccg
     2221 ggatctgaac tgccccctgt gaattcagct cggttgggtt gttccccccg tccactgagg
     2281 ttgaagccgg agatcgtccc gtacggatct ttggggcgta ggacaactct gttctcgttc
     2341 ttcggcgcaa acgaggtctg cgaaccggaa gattgcgtgc ccgacgcctc cgagcccgtg
     2401 tcgccggtgg cctgtgagcc ggtcgacgac gaatccgtgg actgggaggc cgacgactgg
     2461 gatccagccg attgctcggc cgctgggtat ccgttcgggt aagagccggg gtatgagccc
     2521 atcggctgca ggggctggat ggagcccgac gcgttgctcg tcgcgtgctc gaggtactgc
     2581 cgccacgctt gcaggtagcc gaacagttgc tgcgagtagt cggccggcgg gctcgcgccg
     2641 gggggaactg tgccgggggc ggtcatcggc ggcatgaacg gcatcggcgg catcgatggc
     2701 atcggtggca tgcccatcgg acttgggccc attggcatcc cggacgtcag cgccgcggag
     2761 gcctccaaga gttgtcgcca cgcttggaga tagttcagca gttgctggtt gtagttcatc
     2821 gggttcgtca tcagtgatcc tccggccatt cgggttgcag gggtacaggg gtgccgccgt
     2881 cttgatactt cgcgtcgagg ttgcgaagtg ttcgggtgtc gagtggctcg cggttctcga
     2941 gatccatgac atagcagtaa tcgcctgccg gcgaaggcat ccgaaccgga gtcgtcaggc
     3001 cttggtcgat gaagccgtac ggagttcgcc agtcaagcgc tgtctcgccg cgccgatccg
     3061 tgccaccgga ctcgacccag atgtagtgat cgaggatggc gttcaccgcg tgacgtcccg
     3121 actcacatgc cgcctccata gacgtcatgc gagtgaacgt cttggtccac gtgcctgcaa
     3181 acacgaccga gttgttgtga acttgatagc cgccgtgacg ggcctgccag acattgcgct
     3241 gctgtagatc ttcgagccag tcgtcctcgc ctggcacggt ggaccaggag ttgcccgtcg
     3301 ggttccaggg ttcggcgcaa ggccgattgg tccagtcccc gacgatgggc acgaggtacg
     3361 gcgtctcgtt cctgatcgga acgcccgacc cctgaccggg accaccttcc atcatgatgc
     3421 cgcggtcgat ggcgtaccac gcgggccaag gaagcagcga ctcggcgacg ttgggtgagg
     3481 tgctggtgag ggctttgacg atctggtgcc acacttccgt ggcgatttcg tcggccgtgc
     3541 aatggcgggc ggccttgccg tttccggacg agggcgtgtt gaaatcgccg atgtcgaccg
     3601 agagcaccga gacgtggccg tcgcggttca ggatcggttt cttctcccat aggccgtgct
     3661 ggttgatcga cgacagcgcc cacgcagtac ccgagtagta catgtgtcct cgaaggagtt
     3721 gaaactccgt gtcgaagtag tactgaatgc cgccgagcgt ttggaaccgg tcccaggcct
     3781 tgagccccat tgagtccatc gcatagggat cacgccgatc gaccggccgc gtcgagtcgg
     3841 gctgcagcgg gccgtcggga gggggcacga gggtggtgaa tccgtccaac ccggctgcgg
     3901 tcccgccgat gcccgccgcg cgcagcgacg cggtgatgcg ttcggccacc ggcgcgtcga
     3961 cggcgaccac cgtgtaatcg ggggccactc gcgtcccgtc ggcgaacgtg acctgcgcgc
     4021 gtggtctgag gtgcgccggt tgcagtgggt cgaacgccga cgcctcgata ttggcggctt
     4081 ccccgtggac gaagctgact cccagttcga cgaggtggcg gtaccagtgg tcgaaccacg
     4141 cctcggtggt ggggccgttg agcaccccgt cggccttgtt gtcgcgccgg tccatctgca
     4201 gctgaagctg aagataggtg gacacattgg tgcgggcgtc accccagcgg gtatcgaacg
     4261 cagccagcac tctaggcatc tccagcagaa ccgcgttgaa ctgttgcgag tacgagtacc
     4321 gcggaggttc cccgggggcc gcgcggccga tgaaatagtc ataagccgac aggttctgca
     4381 gttctgatgc gcggcgcttg gggctggtga cgaggaagtc gatcagccgg ctcacgaagg
     4441 tctgcacatc cagcggggtg aagcccagct gggtgagctg gcttgcggtg gtgagaaatt
     4501 cggccgggct gcgtggcgcc tcgcggggaa aaaccagcga cggcttgcct tccaacgtgg
     4561 tgccttgggt gaccacccgc ctgaggttgt ccatgatcgt
//
LOCUS       DASYLX010000643         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2226135,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000643 DASYLX010000000
VERSION     DASYLX010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226135"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VGP27_15230"
     CDS             <1..159
                     /locus_tag="VGP27_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEV7852685.1"
                     /translation="SIVMHPNDPNGACGVASDAGGVVGWWVNPQQPGIDACGMALKLM
                     ELTLATSS"
     gene            complement(239..745)
                     /locus_tag="VGP27_15235"
     CDS             complement(239..745)
                     /locus_tag="VGP27_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7852686.1"
                     /translation="MTDRYRTLLLLRHAKSDYPAGVADHQRPLAARGQREAALAGEWL
                     RAHVPAVDAVLCSTATRTRETLARTRIDAPVSYVDRLYDATPGAVIDEINGVDSEVAT
                     LLVIGHEPAMSSVALGLATPEGSNTTAAEHISTKFPTSAIAVLRTAEPWEQLTLSSAA
                     LVTFHVAR"
     gene            848..1996
                     /locus_tag="VGP27_15240"
     CDS             848..1996
                     /locus_tag="VGP27_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEV7852687.1"
                     /translation="MRFVHTADWQLGMTRYFLNGEAQPRYSAARRDAVAGLGGLADDA
                     GAEFVVVAGDVFEHNQLAPRIVSQSLEAMRAIGVPVYLLPGNHDPLDASSVYTSALFT
                     AERPDNVTVLDRPGVHEVRPGLEIVAAPWTSKAPTTDLLAGVLSDLPADGTTRIVVGH
                     GAVDIFVPDKDRPSLIELASVEAAIERGAVHYVALGDKHSRMRVGTTGRIWYSGSPEV
                     TNYDDIEPDPGHVLLVDIDEADPGRPASVEAPRVGRWRFVTLHHAVDSSRDVADLDIN
                     LDLLSDKERTVVRLALTGSLTVTDKATLDACLDKYARLFAALGLWERQSEIAVIPADG
                     EFDDLGIGGFAAAAVDELVAAARSDGDDADDARAALALLLRLVDRGVA"
     gene            1993..>2408
                     /locus_tag="VGP27_15245"
     CDS             1993..>2408
                     /locus_tag="VGP27_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7852688.1"
                     /translation="MKLHRLALTNYRGITHREIEFPDHGVVVVCGANEIGKSSMIEAL
                     DLLLDAKDRSTKKEVKQVKPTHADVGAEIAAEISTGPYRFVYRKRFHKRAETALTVLA
                     PRREQLTGDEAHDRVRAILDETVDVTLWQAQRVLQAA"
BASE COUNT          393 a          813 c          835 g          367 t
ORIGIN      
        1 tcgatcgtca tgcatcccaa cgacccgaac ggcgcgtgcg gcgtggccag cgatgcgggc
       61 ggcgtggtgg gctggtgggt caacccgcag cagccgggca tcgacgcatg cgggatggcc
      121 ctcaagctga tggagctgac gctggccacc agctcctgac gccgacgagc gctcgcgcgt
      181 gggggcactc cctagcaagc tgtgggccca ccctgccgga agggtagggc gagagagttc
      241 aacgggctac atggaacgtg accagcgcgg cgctgctgag cgtcagctgc tcccagggtt
      301 cggcggtgcg cagcacggcg attgccgacg tcgggaactt cgtcgagatg tgttcggccg
      361 cagtcgtgtt gctgccttcc ggggtggcaa gccccagtgc caccgatgac atcgcgggtt
      421 cgtggccgat caccaacagg gtcgctacct cagaatcgac accgttgatc tcatcgatca
      481 ccgctcccgg cgttgcgtcg tacagccggt ccacatagct gaccggcgcg tcgatccgcg
      541 tgcgagccag cgtctcgcgg gtgcgggtgg ccgtcgagca cagcaccgcg tcgacggcgg
      601 ggacgtgagc gcgcaaccac tctcctgcca gcgccgcctc ccgctgtccc cgcgcggcca
      661 gcgggcgctg atggtcggcc accccggccg gataatccga cttcgcatgc cgaagcaaca
      721 gcagcgtgcg gtagcggtcg gtcacgggtc aaccgtaggg catgccgacg cgcgtcccgg
      781 gtcttgtcgg cgaccggacc tacactcgcc acacctagca aagatgcggc acgggaggag
      841 ggtcgaagtg agattcgtgc ataccgccga ctggcagctc ggtatgaccc ggtacttcct
      901 caacggcgag gcgcagccac ggtattcggc cgcccggcgc gatgccgtgg ctgggctggg
      961 tggcctggcc gacgacgcgg gtgctgagtt cgtcgtggtg gccggcgacg tgttcgagca
     1021 caaccagctg gcgccgcgga tcgtcagtca gtcgctcgag gccatgcggg cgatcggcgt
     1081 tccggtctac ctgctgccgg gcaaccacga tccgctcgac gcgtcgtcgg tgtacaccag
     1141 cgcgctgttc accgccgaac gcccggacaa cgtcacggtc ctcgatcgcc ccggtgtgca
     1201 cgaagtgcgg cccggcctgg aaatcgttgc cgcgccgtgg acttcgaagg cgccgaccac
     1261 cgacctcctc gccggggttc tcagcgatct gcctgccgac ggaaccacgc gcatcgtggt
     1321 tggccacggc gccgtcgaca tcttcgttcc cgacaaggac cggccgtccc tgatcgaact
     1381 cgcgtcggtg gaagcggcga tcgagcgcgg cgcggtgcac tatgtcgctc tgggcgacaa
     1441 gcactctcgc atgcgggtcg ggacaacggg ccgcatctgg tattcggggt cgcccgaagt
     1501 caccaactac gacgacatcg aaccagaccc cgggcatgtg ctgctggtcg acatcgacga
     1561 ggccgacccg ggcaggcccg ccagtgtcga ggcgccgcgg gtgggccgct ggcggttcgt
     1621 caccctgcat cacgcggtgg acagcagccg cgacgtcgcc gacctcgaca tcaacctcga
     1681 cctgctgtct gacaaggagc gcaccgtcgt tcggctggca ctcaccggat cgctcacggt
     1741 gaccgacaag gcgacgttgg atgcctgcct ggacaagtac gcgcgactgt tcgcggccct
     1801 cggcctgtgg gagcggcagt ccgagatcgc ggtgatcccg gccgacggcg agttcgacga
     1861 cctcggcatc ggcggtttcg ccgccgcggc cgtcgacgaa ctggtggccg cggcgcgctc
     1921 cgacggcgac gacgccgacg atgccagggc ggctctggca cttctgctgc gtcttgtgga
     1981 caggggtgtc gcgtgaagct gcatcggctc gccctgacca actaccgcgg catcacccac
     2041 cgcgagatcg aattccccga ccacggggtg gtggtggtct gcggcgccaa cgagatcggc
     2101 aagtcgtcga tgatcgaggc gctggatctg ctgctggacg ccaaggacag gtcgacgaag
     2161 aaggaagtca aacaggtcaa gccgacccac gccgacgtcg gcgccgagat cgccgccgaa
     2221 atcagtacgg gcccttaccg gttcgtctac cgcaagcggt tccacaaacg cgcggagacc
     2281 gcgctcacgg tgctggcgcc gcggcgcgaa cagctgaccg gcgacgaggc acacgaccgg
     2341 gtgcgggcga tcctcgacga aaccgtcgac gtgacgctgt ggcaggcgca acgcgtgttg
     2401 caggcggc
//
LOCUS       DASYLX010000644         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_471966,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000644 DASYLX010000000
VERSION     DASYLX010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471966"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VGP27_15250"
     CDS             <1..1288
                     /locus_tag="VGP27_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852689.1"
                     /translation="KDDDADVRVDMLEMLPTPWGLVRSGVAPDHPKIKSISAQFEKTA
                     LDPRFRFFGNIVVGDHVQSAELAERYDAVIYAVGAQSDRPLNIPGEDLAGSVAAVDFV
                     GWYNAHPHFEEMAPDLSTGRAVVVGNGNVALDVARILVTDPDVLATTDIADHALQVLH
                     DRGVEEVLIIGRRGPLQAPFTTLELRELGHLEGLGDVDVIVDPDDFADISDEDLEAAG
                     KTVRNNIKVLRGYAEKAPKGATRRIVFRFRTSPIEIKGDGKVESIVLGRNELVADDTG
                     RVSAKDTGEREELAAQLVVRAVGYRGVETPGLPFDEKNGTIPHAGGRVEGSRNEYVVG
                     WIKRGPTGVIGSNKSDSQETVDTLVGDLAGAELADFDDNHFETLAEWFVERQPKVVTD
                     DHWKLIDDHERSTGGTGGRPRVKLTSVAELLRIAHG"
     gene            1302..>1690
                     /locus_tag="VGP27_15255"
     CDS             1302..>1690
                     /locus_tag="VGP27_15255"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV7852690.1"
                     /translation="MFDRFTATARRVIVSSQSEAQALRQDHVGSHHLLVGVLTAEAAT
                     AGAVLASLGIEAEDVRRRAVAKYGSGEEEPSGHIEFSGDAKKTLEHSLRESLALGHTY
                     IGTEHIVLGLISEPSTCETFAALGVQA"
BASE COUNT          301 a          537 c          591 g          261 t
ORIGIN      
        1 caaggatgac gacgccgacg tgcgggtcga catgctcgag atgctgccga cgccctgggg
       61 cctggtgcgc tccggtgtgg cgccggacca tccgaagatc aagtcgatca gcgcccagtt
      121 cgagaagacg gcgctcgatc cgcgcttccg gttcttcggc aacatcgtgg tcggcgatca
      181 cgtgcagagc gccgagctgg ccgagcgcta cgacgcggtg atctacgccg tcggtgcgca
      241 gtccgaccgc ccgctgaaca tccccggcga ggatctggca ggcagcgtcg ccgccgtcga
      301 cttcgtcggc tggtacaacg cccacccgca cttcgaggag atggcgcccg acctgtccac
      361 cggccgggcg gtggtcgtcg gcaacggcaa cgtcgcgttg gacgtggcgc gcatcctggt
      421 caccgatccc gatgtgctgg ccaccaccga catcgccgac cacgcgctgc aggtgctgca
      481 cgaccgcggg gtcgaggagg tgctcatcat cggcaggcgc gggccgctgc aggcaccgtt
      541 cacgacgctg gagttgcgcg agctaggcca tctcgagggt ctcggagacg tcgacgtcat
      601 cgtcgacccc gacgatttcg ccgacatcag cgacgaggac ctggaggccg cgggcaagac
      661 cgtccgcaac aacatcaagg tgttgcgggg ctacgccgag aaggcgccga aaggggcgac
      721 gcggcggatc gtgttccgat tccgcacgtc gccgatcgag atcaagggtg acgggaaggt
      781 cgagtcgatc gtcttgggcc gcaatgaact tgtcgccgac gacaccggcc gggtttccgc
      841 caaggacacc ggcgaacgcg aggagcttgc cgcgcagctg gtcgtccgcg cggtcggcta
      901 ccgcggggtg gagacgcccg gcctgccgtt cgacgagaag aacggcacca ttccgcatgc
      961 cggcggtcgt gtggaaggaa gccgcaacga atacgtcgtc ggctggatca agcgcgggcc
     1021 gacgggcgta atcggcagca acaagagcga ttcacaggag accgtcgaca cgttggtggg
     1081 cgacctggcc ggcgcggagc ttgccgattt cgatgacaac catttcgaga cgcttgccga
     1141 gtggttcgtc gagcggcagc ccaaggtcgt caccgacgac cactggaagc tcatcgacga
     1201 ccacgagcgt tcgaccggtg gaaccggggg caggccgcgg gtgaagctga ccagcgtcgc
     1261 ggagctactg cggatcgccc acggctgagg ttgcggccgt catgttcgat cgattcaccg
     1321 cgactgcccg tcgggtgatc gtttcatcgc agagcgaggc gcaggcgctt cggcaggatc
     1381 acgtcggttc ccaccatctc ctggtcggcg tactgacggc tgaggctgcg acggcgggtg
     1441 ccgtgctggc gtcactcggt atcgaggcgg aagacgtgcg gcgccgggcc gttgcgaagt
     1501 acggcagcgg cgaagaggag ccgtcggggc acatcgagtt ctccggcgac gccaagaaga
     1561 cactcgagca ctcgcttcgc gagtcgttgg ccctgggcca tacctacatc ggcaccgaac
     1621 acatcgttct cggattgatc agcgaaccct cgacatgcga gacgttcgca gcactgggtg
     1681 tgcaagccga
//
LOCUS       DASYLX010000645         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1458727,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000645 DASYLX010000000
VERSION     DASYLX010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458727"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGP27_15260"
     CDS             <1..201
                     /locus_tag="VGP27_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852691.1"
                     /translation="VDATLVRMVLVPAFMHVMGRWNWWAPGWLTALHRRLGLNDGHSP
                     APPAPAEPVDADIEPRRPGGRV"
     gene            205..1275
                     /locus_tag="VGP27_15265"
     CDS             205..1275
                     /locus_tag="VGP27_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV7852692.1"
                     /translation="MSPSSIIAGVHGALPLYRYAQDEITEAFLRIPAFAGYEQVLRGL
                     HKSAKVDSRYSILPLEELMSLADFKESNDLFIEHAVELGCQALSGALDEAGLRPSDVD
                     LIVTTTVTGVAVPSLDARIAGRLGLRPDVRRVPIFGLGCVAGAAGVARLHDYLRGAPD
                     DVAALVSVELCSLVYPAAQPTIATLVGSALFGDGAGAVVSVGERRAERLGAIGPDVLA
                     SRSRLYPDSLGTMGWDIDGSGFHLVLQPDLPDLIERYFADDVTGFLTDHDLTIDDIGS
                     WVSHPGGPKVLESINTALNLDDDALELSWRSLSEVGNMSSASVLHILRDARDKPPPAG
                     TPGLMMAMGPGFCAELVLLAWN"
     gene            1282..1785
                     /locus_tag="VGP27_15270"
     CDS             1282..1785
                     /locus_tag="VGP27_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV7852693.1"
                     /translation="MTWYALLIAAVAIERVAELVVSQRNLTWSRERGGTEFGGRHYPA
                     MVVLHVGLLVGCLVESLHRPFLPALGWPMLAVVLAAQGLRWWCIATLGHQWNTRIVVV
                     PGAERVSRGPYRLMPHPNYVAVVVEGVALPLAHSAWITALVFTALNAAVLRTRISVEN
                     AALMSLR"
     gene            1782..2798
                     /locus_tag="VGP27_15275"
     CDS             1782..2798
                     /locus_tag="VGP27_15275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7852694.1"
                     /translation="MIDLLVAGGGPAGLATAIHAARAGLEVVVVERRRGPVDKACGEG
                     LMPHTLRQLGALGANPPGMPFHGITYIDGQRRVTGRFRSGPGRGVRRTCLHASLLDAA
                     TAAGVRIQHGEIGRVSQDATSVRCGDFRARHLAAADGLHSPIRASLRLSRPSAGARRW
                     GIRRHIAMKPWTDCVQVHWSDNAEAYVTPVAEDCVGIAILTTRRGPFCEQFNAFPALK
                     ALVDGHAHSPDRAAGPLRQRVRSRSAGRVLLVGDAAGYVDALTGEGLGIAFGAAELLV
                     NCIAAGRVDDYDRQWRQMSRRYRLLTAGVLRASAFPPLRSAIVPAAKVFPGVFTGAVN
                     MLAN"
     gene            complement(3133..>4263)
                     /locus_tag="VGP27_15280"
     CDS             complement(3133..>4263)
                     /locus_tag="VGP27_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7852695.1"
                     /translation="SEFASRVRDADDGVRVVDAADVDMREGSRPAVARAGRIVLLTSG
                     TTGAPKGVPRQPKIRMALGIGASILDRTGLRIGSRISVPVPMFHGLGFGLLALTLGLG
                     GTVLTGRRFDAEATLAQASEHRADALTVVPVMLARILDVADDVRARNPVPSLRVVVSS
                     GARLDPSLAGRFSEVYGDIIYNGYGSSEVGIGALATPMDLREAPETVGRPVVGCAVRI
                     LNQDGGVVGPEVTGRVFVGGDLTFEGYTGGENKEVVDDMMDTGDMGYLDRAGRLFIVG
                     RGDDMIVSGGENVYPRAVENALAEHPDVADNAVIGVPDEDYGQRLVAFVVPRADADVR
                     EDSIREFLKDRVSRFEQPRDIRIVKEIPRNPAGKVVRNELTP"
BASE COUNT          680 a         1436 c         1394 g          753 t
ORIGIN      
        1 gtcgacgcga cgctggtgcg gatggttctg gtgcccgcgt tcatgcacgt catgggccga
       61 tggaactggt gggcacccgg gtggctgacc gcactgcatc gccgcctcgg gctcaacgac
      121 ggccacagcc cggcgccgcc agcaccggcc gaaccagtcg acgccgacat tgaaccgagg
      181 cgccctgggg gtcgtgttta gaggatgagc ccttccagca tcattgcggg cgtgcacggg
      241 gcgttaccgc tttaccggta tgcgcaggat gaaatcaccg aagcgttcct gcggattccg
      301 gctttcgccg gatatgaaca ggtcctacgt ggactccaca agagtgcgaa ggtcgacagc
      361 cgctacagca tcctgccgtt ggaggagctg atgtcgctcg ccgacttcaa ggaatccaac
      421 gacctcttca tcgagcacgc cgtcgagctc gggtgccagg cgttgtccgg tgcgcttgac
      481 gaggcggggc tgcgccccag tgacgtcgac ctgatcgtga ccacgaccgt gaccggtgtg
      541 gcggtgccgt cgttggatgc ccggatcgcc gggcggctcg gcctgcgacc cgacgtgcgc
      601 cgggtgccga tcttcggcct cggctgcgtg gccggcgcgg ccggcgtggc ccgtctgcac
      661 gactatctgc ggggtgcacc cgacgacgtt gccgcgctgg tgtcggtcga gctgtgctcg
      721 ctggtctacc ccgccgctca accgacgatc gccacgctgg tgggcagcgc attgttcggc
      781 gacggggcgg gcgccgtcgt gtcggtcggt gagcgtcgcg ccgagcgctt gggggccatc
      841 gggccggacg tgctggcctc acgtagtcgg ctctaccccg actcgctcgg cacaatgggc
      901 tgggacatcg acggttcggg ctttcatctg gtgctccagc ccgatctgcc cgacctcatc
      961 gagcggtatt tcgccgacga cgtcaccggc ttcctgaccg atcacgacct gacgatcgac
     1021 gacatcggtt cctgggtgtc gcaccccggc ggcccgaagg tcctcgagtc gatcaacacc
     1081 gccctcaacc tcgacgacga cgcgctggag ttgtcgtggc gctccctgag cgaggtcggc
     1141 aatatgtcgt ctgcctcggt gctgcacatt ctgcgcgacg cgcgtgacaa gccgccaccg
     1201 gccggcacac cggggctgat gatggcgatg ggcccgggct tctgcgccga gctggtgctg
     1261 ctggcctgga actgaacgtt gatgacctgg tatgcgttgt tgatcgccgc ggtggccatc
     1321 gagcgtgtcg cagaactcgt tgtctcgcaa cgcaatctga cctggagccg ggagcgcggc
     1381 ggcaccgagt tcggaggccg gcactatccg gcgatggtgg tcctgcatgt gggcctgctg
     1441 gtcggttgcc tggtcgagag tctgcaccgc ccgttcctgc ccgcgctggg ctggcccatg
     1501 ctggcggtgg tgctggccgc acagggcctg cggtggtggt gcatcgccac ccttggccac
     1561 cagtggaaca cccgcatcgt cgtcgtcccc ggcgccgagc gggtgagccg tgggccgtat
     1621 cgactcatgc cgcacccaaa ctacgtagcg gtggtcgtcg aaggggttgc actgccgctg
     1681 gcgcatagcg cgtggatcac cgcgctggtg ttcaccgcgc tcaacgctgc ggtgctgcgg
     1741 acgcggatca gtgtcgagaa cgctgccctg atgagcctgc gatgatcgac ctgctggttg
     1801 cgggtggcgg gccggccggt ctggccaccg caatccatgc cgcacgggcc gggctcgagg
     1861 tcgtcgtggt ggaacggcgg cgcgggcctg tcgacaaggc ctgcggagag gggctgatgc
     1921 cccacacgct tcggcagttg ggagcgctcg gcgcaaaccc gccgggcatg ccgttccacg
     1981 gcatcaccta catcgacggt cagcgccggg tgaccgggcg attccgctcc gggccgggcc
     2041 gcggtgtgcg ccgaacatgt ttgcatgcaa gccttttgga tgcggcgacg gcggcaggcg
     2101 tgcggatcca gcatggcgag atcggccggg tgagtcagga cgcgacgtcc gtgcgctgcg
     2161 gcgacttccg cgcccgccac ctggccgccg cggacggact ccactcgccg atccgggcct
     2221 cgctccggct gagccgcccc tccgcggggg cacgcaggtg gggtatccgc cgtcacatcg
     2281 cgatgaagcc gtggactgac tgcgtgcagg tgcattggtc tgacaatgca gaggcgtacg
     2341 tgacgccggt cgccgaggac tgtgtcggca tcgcgatcct caccacgcgc cgcggcccat
     2401 tctgcgaaca gttcaacgca ttccccgctc tgaaggctct ggtcgacggc cacgcacaca
     2461 gcccggatcg ggcagcgggt ccgctgcggc aaagagtgcg cagccgctca gcgggacggg
     2521 tcctgttggt cggtgacgcg gcgggatatg tcgacgcact gactggcgaa ggcctcggca
     2581 tcgccttcgg cgcggccgag ctgctcgtga actgcatcgc cgccgggcgt gtcgacgact
     2641 acgaccgtca gtggcgccaa atgtcacgcc gctaccggct gctcaccgct ggtgtgttgc
     2701 gcgccagtgc gttccctccg ctgcgttctg caattgtgcc cgccgccaag gtctttcccg
     2761 gcgtcttcac gggcgcggtc aacatgctcg ccaactagca gccatcgttg cgtcagtcgt
     2821 ctaatcaagc ccaccactct aagtcgcaga caggagcgct tctaactcag gctcgacggg
     2881 gtcggccaga tcccgcctac aacgctgccc accaggtgct cgacggcatc ggcgacccga
     2941 tcatcgcatt tcatgactta atcgcaattc accctgtatc gaacagttac ccaacaccgc
     3001 gagacattgc gtagcgtttt ccgctaattg ccgacgcgac gctgcctggg gggcgaatgg
     3061 cttttccgtg ggaattgaat ctcgaaccta cataccgtga cgagaagcca ccttctcgcg
     3121 aatgcgatgt tgtcagggcg tcagctcgtt ccgaacgacc ttacccgccg gattgcgcgg
     3181 aatctccttc acgatcctga tgtcgcgagg ttgctcgaaa cgggaaaccc tgtctttcag
     3241 gaactctcga attgagtctt cgcggacgtc ggcgtccgct cgcggtacga cgaacgccac
     3301 cagtcgttgc ccgtagtcct cgtcgggcac gccgatcaca gcgttgtcgg cgacgtccgg
     3361 atgctcggcc agggcgtttt ccaccgcacg cggatagacg ttttcgcccc cggagacgat
     3421 catgtcgtct ccgcggccga cgatgaacag ccgtcccgcc cggtcgaggt agcccatgtc
     3481 gccggtgtcc atcatgtcgt cgacgacctc tttgttttca ccgcccgtgt aaccctcgaa
     3541 cgtcaagtcg ccgccgacga aaacccgccc ggttacctcg gggccgacaa ccccgccgtc
     3601 ctggttgagg atccgcaccg cgcagccgac aacgggtcta cccaccgtct ccggcgcttc
     3661 ccgaaggtcc atcggcgttg cgagagcgcc gatcccgact tccgacgagc cgtaaccgtt
     3721 gtagatgatg tcgccgtaaa cctccgagaa ccgtcccgcc agactcggat caagccgggc
     3781 gccgctcgat accaccactc gtaacgacgg cacgggattt cgcgctcgca cgtcatcggc
     3841 gacgtcgagg atgcgcgcaa gcatgaccgg cacgacagtc aacgcatcgg cgcgatgttc
     3901 ggaggcctga gccagcgtgg cttcggcgtc gaagcgccga ccggtgagca cagtgccgcc
     3961 aagaccaagg gtcagggcca gcaagccgaa acccaagcca tgaaacatgg gcacaggcac
     4021 tgagattcgt gatccgatcc gaagccccgt gcgatccaag atagatgcgc cgatgcccaa
     4081 tgccatccgg attttcggct gacggggcac acccttgggt gcaccggtcg tgccggaggt
     4141 cagcagaacg attcggccgg cccgcgccac agcaggccgc gaaccttcac gcatatcgac
     4201 atccgccgca tcgaccaccc tgaccccgtc gtccgcgtca cgcacgcgcg aagcgaactc
     4261 gct
//
LOCUS       DASYLX010000646         6892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_143295,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000646 DASYLX010000000
VERSION     DASYLX010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143295"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VGP27_15285"
     CDS             complement(<1..915)
                     /locus_tag="VGP27_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused (3R)-hydroxyacyl-ACP dehydratase subunits
                     HadA/HadB"
                     /protein_id="HEV7852696.1"
                     /translation="MTAPAEASALEARVGHYYQMDGTYLVGREKLREYARAVQDYHPA
                     HWDVAAAAELGYSDVVAPLTFTSTPGMTCNRRMFEQVVVGYDTYMQTEEVFEQHRPIV
                     AGDELHIDVELTSLRRIAGRDLITVTNTFTDMAGERVHTLHTTVVGLTTEDVDPAIKT
                     AVQKMMMHDVDIFGIGESEYQKTVRPAGEVRLAEGGKTRTPGTPSFDDVQVGDELPVR
                     HTRVSRGDLVNYSGVAGDANPIHWDESIAKLAGLPDVIAHGMLTMGLGAGFASAWLND
                     PGAVTRYAVRLSAPAIVSAAEGADIEFSG"
     gene            complement(1023..1352)
                     /locus_tag="VGP27_15290"
     CDS             complement(1023..1352)
                     /locus_tag="VGP27_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852697.1"
                     /translation="MTAGIVTTVAGSALLCAPGRMGPLIGLMSRRDVRLVGALDLALA
                     PGLMFGRPQWPWLAARAVSNVATGLFVFLRAEDGGPVRNARVFSAFLAAATLADLRTF
                     RALALTK"
     gene            complement(1459..2454)
                     /locus_tag="VGP27_15295"
     CDS             complement(1459..2454)
                     /locus_tag="VGP27_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEV7852698.1"
                     /translation="MVSSAAGAVARWIFPFLAVAAVVSMGVSADPVRVGPAPTVRLAS
                     DGNPLAGLPFYVNPSSKARAAADGAGSPELDAIAATPTAYWMDNLSTPAVDAKYIADA
                     QAAGTMPVLALYGIPHRDCGSFAAGGFGSADAYKGWINGVAAAIGGGPAAVILEPDAL
                     AMADCLSGDQRQERFDLMRYAVDTLTVNPATALYVDAGHSRWVGAEDMAGRLNDVGVG
                     KARGFSLNTANFFTTDEEIGYGDAISGLTNGAHYVIDTSRNGAGPADGDMYWCNPSGR
                     ALGAAPTTDTGNGNIDAFLWVKRPGESDGSCGSGEPGAGRFVNSFAIDLARNARR"
     gene            2565..3767
                     /locus_tag="VGP27_15300"
     CDS             2565..3767
                     /locus_tag="VGP27_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7852699.1"
                     /translation="MTETPADVVESVGPPRRRRRHWRRIAVVLAVMLVLFGVPWWTLL
                     ASGQGWPTTVFVTGTLLFTAALVAMPWLMMLGHGRRHLDWAAATGAVLLGAAWVLFVW
                     SVLGQVLRIVLLIAGVADPARSRVVAAAVVAVVAVLLVWGYAEAMRVPRVKTIDVAIA
                     RLGRGLDGLRVAVITDTHYGPIDRARWSAAVVARVNALEADVVCHVGDIADGTVDVRE
                     AQASPLAAVDATKARLYVTGNHEYFSEAQGWLDYMEAIGWTALHNRHLILERGGDRLV
                     VAGVDDATAKASGVRGHGANLEAALAGADSTLPILLLAHQPKQVALAADQGIDLQISG
                     HTHGGQIWPFNFLVRLEQPVVHGLSRHGDRTQLYTSRGTGFWGPPFRVFAPSEITLLT
                     LRRSVGVG"
     gene            3811..4671
                     /locus_tag="VGP27_15305"
     CDS             3811..4671
                     /locus_tag="VGP27_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7852700.1"
                     /translation="MIEHKSIDIHGEQLAYYDEGQGEAILLVHGMAGSSQTWREILPR
                     LAKNYRVIAPDLLGHGQSAKPRTDYSLGAFAVGLRDLLDELEVDSATVVGHSLGGGIA
                     MQFLYQHPDYCRRLILISSGGLGQDVGWILRLLSAPGAELVMPVIAPKLVLRAGDSVK
                     TWLSSMGLRSPRGAEIWNAYSSFSDAQTRESFLRTLRSVVDYRGQSVSALNRLQLRAD
                     LPTLAIWGEDDNIIPVDHAYAALEARPDCRLEVLPGVGHFAHVEAPTQVVDLIDEFMA
                     ETSAAVPHSG"
     gene            4733..5770
                     /locus_tag="VGP27_15310"
     CDS             4733..5770
                     /locus_tag="VGP27_15310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV7852701.1"
                     /translation="MKNLAAARSRHPLRSILVALLGVLTAAVLSISTPHASALPVLAA
                     DTAGVEPAVVRIDTKLDFQSAVGAGTGVVLSPDGIVLTNNHVIRGANDITATNVGNGQ
                     TYPVDVLGYDRKSDIAVLQLRGAQNLPVAPTADSTGLQVGDAVTAVGFPEGAGLTRAP
                     GTVRALNQSIVANDDLTGSSEELSDLVDFAADIRPGDSGGPLVDAGGRVVGIVTAASQ
                     NYRMESTGGFAIPLNRALSLVDAVRSGNPSGSVHIGPTGILGVAIRSGRNGAGVPVEG
                     VLRGSAADQAGLRGGDVITAIDDTTITDGTVLTDVLDGHHPGDVVTLTYVDRAGNPRN
                     VPVTLSAGPPN"
     gene            complement(5704..>6892)
                     /locus_tag="VGP27_15315"
     CDS             complement(5704..>6892)
                     /locus_tag="VGP27_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7852702.1"
                     /translation="TALTANVLLQTYNPVRVGLLYLPELGGAIVMAVVFGFVISRRAM
                     HYLPLVGMALLAAGILVYRMALPANQPLALLGAGLTGLALGATVAPALFVAGFSLQSA
                     SLQRVFAIIELLRAVAAFMVAPILAHIADALPGDLNAGTGITLWIGFGLAVGGAVFGV
                     AVYALSGARPQTPDLDEFLDGESPAWYSPPLLARLRNLPTSVPVTEPTPVTSSADAGF
                     RPASAGGPVLLAYDGTELAACAIRMAAAQLAPYRDALVVCVWQTVDVGFTPSDTTHFD
                     AGQATEVRRAAERTAACGADLAARAGFRARSMAIEAAPTWKGIVRAADENAASMIVLG
                     PHRRSGLLGHLQGSVAAAVVTHTDIPVLLIPEQPCRVPSAQFGGPAESVTGTLRGLPA
                     RST"
BASE COUNT         1124 a         2303 c         2337 g         1128 t
ORIGIN      
        1 gccgctgaat tcgatgtccg cgccctcggc ggccgacacg atcgcgggtg ccgacagccg
       61 caccgcgtag cgggtgaccg caccgggatc gttcaaccac gccgaggcga atccggcacc
      121 cagacccatg gtgagcattc cgtgggcgat cacatcgggc agcccggcca gcttggcgat
      181 gctctcgtcc cagtgaatcg ggttggcgtc gccggccacg ccggaatagt tcaccaggtc
      241 gccgcgcgac actcgggtgt ggcgcaccgg caactcgtcg ccgacttgca cgtcatcgaa
      301 ggacggcgtt cccggcgtgc gggtcttgcc gccctcggcg agccgaacct cgcccgcggg
      361 gcgcaccgtc ttctggtatt cggattcgcc gattccgaag atgtccacat cgtgcatcat
      421 cattttctgc accgcggtct tgatcgccgg atcgacgtcc tcggtggtga ggccgaccac
      481 ggtggtgtgc agggtgtgca cccgctcgcc ggccatgtcg gtgaaggtgt tggtcacggt
      541 gatgaggtcc ctgcccgcga tcctgcgcaa ggacgtcagt tcgacgtcga tgtgcagttc
      601 gtcgccggcc acgatcgggc ggtgttgctc gaagacctct tcggtctgca tgtaggtgtc
      661 gtagccgacg acgacctgtt cgaacatgcg gcgattgcag gtcatgccgg gagtcgacgt
      721 gaacgtcagc ggcgccacca cgtccgaata tcccagctcc gcggcggcgg cgacatccca
      781 gtgcgcgggg tggtagtcct gcacggcacg ggcgtactcg cgtaacttct cgcggccgac
      841 aaggtaggtg ccgtccatct ggtagtagtg gccgacccgc gcttcgagcg ccgacgcctc
      901 tgctggtgcg gtcatcaatt cactcaactc ttctatcggc ttgttcgctg cggccgacca
      961 aagcacacta acgcccgaac gcggcggcaa tggacggtgg gttcgccctg cccgatcgtc
     1021 gactacttgg tgagagccag cgcgcgaaag gttcgcagat cggccagtgt ggcggcggcc
     1081 aggaaggcgc tgaacacccg tgcgtttcgc acgggcccac catcctcggc gcgcagaaag
     1141 acgaaaagcc ctgtcgctac gttgcttaca gcccgagctg cgagccatgg ccactgcggc
     1201 ctgccaaaca tcagacccgg cgccagtgcg aggtccagag ctcccacgag ccgcacatca
     1261 cgtctcgaca tcagcccgat cagcgggccc atccggccgg gcgcgcacag cagcgcgcta
     1321 ccggcgacgg tggtgacaat gccggccgtc atcgagagtg attgtgcaga tgcgcggccg
     1381 gtggatggtg tcacgatcgc aattatccgc gtcaggccga atctcgctga gcgggtcaag
     1441 acccgaagcg gagcgtcgct accggcgggc gttgcgggcc aggtcgatgg cgaacgagtt
     1501 cacgaagcgc cccgcgccgg gttcgccgct tccgcacgag ccgtcggact cgccgggacg
     1561 tttgacccac aggaaggcgt cgatgttccc gttcccggta tccgtcgtcg gcgcagcacc
     1621 gagcgcccgc ccgctcgggt tgcaccagta catgtcaccg tccgccggac ctgcgccgtt
     1681 gcgcgatgtg tcaatcacat agtgggcacc gttggtgagg ccggaaatcg cgtcgccgta
     1741 gccgatttcc tcgtcggttg tgaagaagtt cgcggtgttg aggctgaagc cccgtgcctt
     1801 gccgacgccg acgtcgttca gcctgcccgc catgtcctcg gcgcccaccc agcgcgagtg
     1861 tcccgcgtcg acatacagcg cggtggcagg gttaacggtc agcgtgtcga cggcgtaacg
     1921 catcaagtcg aagcgttcct ggcgctggtc acccgacaga cagtcggcca tggcgagcgc
     1981 gtcgggttcg aggatgaccg ccgccggtcc gccgccgatg gctgctgcga cgccgttgat
     2041 ccagcctttg taggcgtcgg ccgacccgaa cccgcctgcg gcgaagctcc cgcagtcacg
     2101 gtgcgggatg ccgtagagcg cgagcaccgg catggtgcct gcggcctgtg catcggcgat
     2161 gtacttcgcg tccacggccg gagtggagag gttgtccatc cagtaggccg tcggcgtagc
     2221 tgcgatggcg tccagctcgg gactcccggc tccgtcggct gccgcccgcg ccttcgatga
     2281 cgggttgacg tagaagggca ggccggccag cgggttcccg tcgctggcaa ggcgcaccgt
     2341 cggagccggg ccgactcgca ccgggtcggc agacacaccc atgctgacga cggcggccac
     2401 cgccaggaac ggaaagatcc atcgcgcgac tgcgccggct gctgaggaaa ccaccccgaa
     2461 aacatagtgc agcggcaagc tcaacgcgcg ctcggccggt gctccactgc gcggcgtcgg
     2521 taacgtcgtc tatggcactg agcccgcaag aagatcgagg cgcgatgaca gaaaccccgg
     2581 cagatgtagt cgagtccgtc ggcccgccgc gacggcgccg gcggcattgg cggcgcatcg
     2641 cagtcgtgct cgccgtcatg cttgtgctgt tcggcgtgcc gtggtggacg ctgctcgcat
     2701 cggggcaggg ctggccgacg accgtcttcg tcaccggcac gctgctcttc acggccgccc
     2761 tcgtcgcgat gccgtggttg atgatgctcg gtcacggccg taggcacctc gactgggccg
     2821 cagcgaccgg agccgtactg ctcggcgcgg cctgggtgct gttcgtgtgg tcggtgctcg
     2881 gccaggtgct gcgaatcgtg ttgctcatcg cgggtgtcgc ggatccggca cgctcgcggg
     2941 tggtcgcagc tgcggtcgtg gccgtagtgg ccgtgctcct ggtgtggggc tatgccgaag
     3001 cgatgcgggt gccgcgggtc aagacgatcg atgtggccat cgcccgactc ggccgcgggc
     3061 tcgacggcct gcgcgtggcg gtgatcaccg acacgcacta cggaccgatc gatcgcgcac
     3121 ggtggtcggc agccgtggtg gcgcgggtga acgcactcga agccgatgtc gtgtgccacg
     3181 tcggcgacat cgcggacggg acggtcgacg tccgggaggc gcaagcgagc ccgctggccg
     3241 cggtggacgc gacgaaggcc cgcctgtatg tgaccggcaa ccacgagtac ttcagcgagg
     3301 cgcagggctg gctggattac atggaggcca tcggctggac cgccctgcac aaccggcacc
     3361 tcatcctgga gcgcggcggc gaccggctcg tggtggccgg ggtggacgac gccactgcga
     3421 aggcgtcggg tgttcgcgga cacggtgcga atctcgaggc tgcccttgcg ggagccgact
     3481 ccacgctccc gattcttctc ctcgcgcatc aacccaagca ggtcgcgctc gcggcggacc
     3541 agggcatcga cctccaaatc tccgggcaca ctcacggtgg ccagatctgg cccttcaact
     3601 ttctcgttcg attagagcaa ccggtcgtgc acggtctgag tcgacacggc gaccggacac
     3661 agctgtacac cagccgcggg acaggcttct ggggcccgcc gttccgggtg ttcgcgccca
     3721 gtgagatcac tttgctgaca cttcgccgaa gcgtaggcgt cgggtgaagt tacccgccgg
     3781 tagaatggcg tcgggagaac ggaggcgttc gtgatcgaac acaagtccat cgacatccac
     3841 ggcgaacaac tggcctacta cgacgagggt caaggcgagg ccatcctgct ggtccacggt
     3901 atggcgggaa gttctcagac gtggcgcgag atcctgcctc ggctcgccaa gaattaccgg
     3961 gtgatcgcgc ctgacctgtt gggccacggc caatccgcca aaccgaggac cgactattcg
     4021 ctcggagcgt tcgccgtcgg cttacgcgac ctgctcgacg aactcgaggt ggactcggcg
     4081 accgtcgtcg gccactctct cggcggcggt atcgcgatgc agttcctcta ccaacatccc
     4141 gactactgcc gtcggctcat cctcatcagc agtggcgggc tgggccagga cgtcggctgg
     4201 atcctgcgct tgctctccgc gccgggagcc gagctggtga tgccggtgat cgcgccgaaa
     4261 ctcgttctgc gcgctggcga ttcggtgaaa acctggttgt cgtcgatggg gttgcgttca
     4321 ccgcgcggcg ctgaaatctg gaacgcgtat tcgtcgttct ccgacgccca gacacgcgaa
     4381 tcgttcctgc gcacactgcg ctcggtcgtc gattaccggg ggcagtcggt cagcgccttg
     4441 aaccgattgc agctccgggc cgatctgccc acattggcga tctggggcga ggacgacaac
     4501 atcatcccgg tcgatcacgc gtacgcggcg ttggaggcgc ggccggactg ccgcctcgag
     4561 gtgctgccgg gcgttggtca tttcgcacac gtagaagccc ccacgcaggt tgtcgatctc
     4621 atcgacgaat tcatggccga gaccagcgca gcggttcctc acagcggctg aacccgcgtg
     4681 acgtcaccgg cgacgacgtt agaacgccta tttggggggt atggagcgca acatgaagaa
     4741 cctcgccgcc gctcgtagcc gccacccgtt gaggtcgatc ctcgtagcgc tactgggcgt
     4801 cctcaccgct gccgtgctga gtatctccac accacacgcg tcggcgctac cagtgctggc
     4861 cgctgacacc gccggtgtcg agccggctgt cgtgcgcatc gacaccaaac tcgactttca
     4921 gagcgcagtc ggtgcaggca ccggcgttgt tttgagcccc gacggcatcg tgctgaccaa
     4981 caatcacgtc atccgcggcg ccaacgacat cactgcgacg aacgtcggca acggtcagac
     5041 gtatccggtg gacgtgctcg gttacgaccg caagagcgac atcgccgtcc tgcagctgag
     5101 gggcgcgcag aaccttcccg tcgcccccac cgccgactcg acgggtctac aggtcggtga
     5161 cgcggtgacc gccgtcgggt tcccggaggg cgcaggcttg acccgcgcgc cgggcaccgt
     5221 cagggcgctg aaccaaagca tcgtcgccaa cgacgatctc accggcagca gcgaggagct
     5281 gtccgatctc gtcgacttcg ccgcggacat ccggccagga gactccggcg gcccgttggt
     5341 cgatgccggc gggcgagtcg tcggtatcgt caccgccgct tcgcagaact atcggatgga
     5401 gagcaccggc ggtttcgcca ttccgctcaa ccgcgccttg tcgctcgtcg acgccgtccg
     5461 ctcgggcaac ccctccggct ccgttcacat cggcccgacc gggatcttgg gcgtcgcgat
     5521 cagaagcggt cgcaacgggg cgggtgtccc cgttgaaggt gtgctgcgtg ggtcggccgc
     5581 cgaccaggca ggcttgaggg gcggcgacgt catcaccgcg atcgacgaca ccacgatcac
     5641 cgacggcacc gtactcaccg acgtcctcga cggacaccac cccggtgatg tcgtgacgct
     5701 gacttacgtt gaccgcgcgg gcaatccgcg caatgtgccc gtgacgctct ctgccggccc
     5761 tccgaactga gcggacggca cccggcacgg ctgctcgggg atgagcagga cgggaatgtc
     5821 ggtgtgcgtt acgacggctg ccgcaacgct gccctgcagg tggccgagca ggccgctacg
     5881 gcggtgaggc ccgagcacga tcatgctggc ggcgttctcg tcggcggccc tgacgatgcc
     5941 cttccacgtc ggcgccgctt cgatcgccat gctccgggcc cgaaagccgg cccgtgccgc
     6001 gaggtcggcg ccgcaggctg cggtccgttc ggcggcacga cggacttccg tggcctggcc
     6061 tgcgtcgaaa tgcgttgtgt cgcttggcgt gaagccgaca tcgaccgtct gccatacgca
     6121 cacgacgagg gcatcgcgat agggcgccag ctgcgcggcg gccatccgta tcgcacacgc
     6181 ggcgagttcg gttccgtcgt aggccaacag caccggcccg cctgctgacg ccggccggaa
     6241 accggcgtct gccgacgagg tgaccggcgt cggctccgtc acaggaaccg aggtcggcag
     6301 gttgcgcagc ctggccagca gcggcggtga gtaccaggca ggcgattcgc cgtcgaggaa
     6361 ctcatccaga tcgggtgtct gcgggcgggc accgctcagc gcgtacacgg cgacaccgaa
     6421 gaccgcgccg ccgacggcca gaccgaaacc gatccacagc gtgatacccg tgcccgcatt
     6481 gaggtcaccc ggcaacgcgt ccgcgatatg ggcgaggatc ggcgcgacca tgaacgcggc
     6541 caccgcgcgc agcagctcga tgatcgcgaa aacgcgctgc aggcttgcgg attgcagcga
     6601 gaaacctgcc acgaacaacg cgggggcgac cgttgcgccc agcgcgagac cggtcagccc
     6661 ggcgccgagc agggccaggg gctggttggc gggcagcgcc atccggtaca ccaggatgcc
     6721 ggccgccaac agggccatgc cgacaagcgg gaggtagtgc atcgcgcgcc gtgagatcac
     6781 gaatccgaag acgacggcca tcacaatggc gccgccgagt tcaggcaagt agagaagccc
     6841 gacccggacg gggttgtagg tctgcaacag cacgttggcc gtcagggcgg tg
//
LOCUS       DASYLX010000647         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_253086,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000647 DASYLX010000000
VERSION     DASYLX010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253086"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VGP27_15320"
     CDS             <1..885
                     /locus_tag="VGP27_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7852703.1"
                     /translation="RMIRRVEDDLRARTRRLLDDVSPGSPFDFVVDVAAELPMQMICI
                     LLGVPESERHWLFEAIEPQFDFGGARKASVGQMSAEEAGTRMYSYGQDLIASKRAEPT
                     DDMLSVVANATLDSDGLSDLELYLFFSLLFSAGAETTRNAVAGGLLVLAEHPEQLALL
                     RADLSLLPTAVEEMVRWTSPSPSKRRTATRDVLLGGCAVEAGQKVQIWEGSANRDASA
                     FDRPGVFDVARKPNPHLGFGQGIHYCLGANLARLELRVLYEELLGRFSSVRVVKPVEW
                     TRSNRHTGIRHLVVELEA"
     gene            882..1808
                     /locus_tag="VGP27_15325"
     CDS             882..1808
                     /locus_tag="VGP27_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEV7852704.1"
                     /translation="MKPDAFAFVMATGIVSIAAADHGFDSISDVLVVLAAAGLVVLAV
                     VGVFVWRRAPLDFSTPEAPIGLLTFVAACAVVGTRLKWSAPVHWSFAALALIGWLVLM
                     ALVLRAMWRCRWAGLRDRARGGGELVSVATSGLAILAADLGLVGVAIALLLLALCLYG
                     LMTGLVLWRVVHERTAPELFAPDMWILMGGAAIATLAGDHIHQAGVEGIRPVTVATWV
                     VAGLWIPALAVASMRLRDGNWWAAVFPLGMYSSATFATAVETGWHPLTWISLAFFWIA
                     FGAWLLTCCLEIDVMARSTRTFSPKRWFGRRG"
     gene            complement(1812..>2032)
                     /locus_tag="VGP27_15330"
     CDS             complement(1812..>2032)
                     /locus_tag="VGP27_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852705.1"
                     /translation="RFFRRKWVALTLARTPAGEQLLAMRALANRPLAKLAAINADPVG
                     AWRREDAEAIQGLAALELRAAGVARRGT"
BASE COUNT          270 a          621 c          739 g          402 t
ORIGIN      
        1 cggatgatcc gccgggtcga ggacgatctg cgcgcccgca cccgtcgtct cctcgacgac
       61 gtttcgccgg gttcgccctt cgacttcgtg gtcgacgtcg cagcggagct gccgatgcag
      121 atgatctgca ttctactggg agttcctgaa tccgaacggc attggctgtt cgaggcgatc
      181 gagccgcagt tcgacttcgg cggcgcgcgc aaggcatccg ttggccagat gtcggccgag
      241 gaggccggga cgaggatgta cagctacggg caggatctga ttgcgtccaa acgcgccgag
      301 ccgactgacg acatgctgtc ggtcgtcgcg aacgcgacgc tggactcgga cggactatcc
      361 gacctcgagc tgtatctgtt cttcagcctg ctgttcagtg cgggcgcgga aaccacccgc
      421 aatgcggtcg cgggcggcct gcttgtgctc gccgagcatc ccgagcagct cgcgttgctg
      481 cgggccgacc tgtcgctgct gcctaccgcc gtcgaggaga tggtgcgctg gacgtcgccg
      541 tcgccgtcta agcgccgtac cgcgacccgc gacgtcttgt tgggtggctg cgccgtcgag
      601 gcggggcaga aggttcagat ctgggagggc tcggccaacc gcgacgcgtc ggcgttcgac
      661 cgtccaggag tgttcgacgt agcgcgtaag ccgaacccgc acttgggttt cggccagggt
      721 attcattact gcttgggcgc caacctcgcc aggttggagc tgcgggtgct gtacgaggag
      781 ctgctcggcc ggttctcgtc ggtccgtgtc gtgaagccgg tggagtggac acgaagcaac
      841 cggcacaccg gcatacgtca cctcgtcgtc gaactggaag cgtgaagccc gacgccttcg
      901 cgttcgtgat ggcgaccggc atcgtttcga tcgcggcggc ggatcatggc ttcgacagca
      961 tcagtgacgt gctcgtggtg ttggcggctg cggggttggt ggtgcttgcc gtggtaggtg
     1021 tattcgtctg gcgtcgtgcg ccattggact tctctacgcc cgaggcgccg atcggactgc
     1081 tgacattcgt tgccgcatgc gccgttgtcg gaacgcggct caaatggtct gcgccggtgc
     1141 actggtcgtt tgcggcactg gcgctcatcg gctggctggt gctcatggcg ctggtgttgc
     1201 gcgcgatgtg gcggtgccgg tgggccgggc ttcgcgaccg cgcccgcggt ggcggcgagt
     1261 tggtcagcgt cgcgacatcg gggctggcga tactcgcagc ggacctgggc ttggtgggag
     1321 tggccatcgc tctgctgctg ttggcgctct gcctgtacgg cttgatgacg ggcctggtgc
     1381 tgtggcgcgt ggtgcacgag cggacggcac ccgaactgtt tgcgcccgat atgtggatcc
     1441 tgatgggcgg ggcggcgatc gcgacgctgg cgggcgatca cattcaccag gcaggagtcg
     1501 agggcattcg accggtcacc gtcgcgacgt gggtcgtcgc cggtctgtgg attccggcac
     1561 tggccgtggc gagcatgcga ctgcgcgacg ggaactggtg ggctgcggtg ttcccgctgg
     1621 gcatgtactc gtcggcgacg ttcgcgacgg ccgtcgaaac ggggtggcac ccgctgacgt
     1681 ggatctcgct ggccttcttc tggatcgctt tcggcgcgtg gctgctcacc tgctgcctcg
     1741 agatcgacgt aatggcgcga tctactcgta ctttctcgcc caaacgttgg tttgggcgga
     1801 gggggtgagg gttaggtgcc gcggcgggcg acgcctgcgg cccgcagctc gagcgccgca
     1861 agaccctgta tcgcctcggc gtcttcacgg cgccatgctc cgacggggtc ggcgttgatc
     1921 gcggccagct tggccagcgg gcggttggcc agcgcccgca tcgcgagcaa ctgttcgccc
     1981 gcaggcgtcc tcgcgagcgt cagcgccacc cacttgcgcc ggaagaaccg ca
//
LOCUS       DASYLX010000648         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_801970,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000648 DASYLX010000000
VERSION     DASYLX010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801970"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..790)
                     /locus_tag="VGP27_15335"
     CDS             complement(5..790)
                     /locus_tag="VGP27_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV7852706.1"
                     /translation="MATKPTKNARYDLKAADRRRNLLIQIGLTAVVVLLGVGLVLYIV
                     MGADKKPASGEAKAITVSSSKLITKEGTTEPKAVVSLYEDFLCPHCGEFEQKFGPTIG
                     QLIDSGAIAADYYMVAILDQPSNDNYSSRAGAAAYCVADESKDAFRRFHAALYAQQPG
                     ETTASFPDNARLIEIARQAGAAGSVPDCINKGKYVPMVQGLAQATQIQSTPTIKINGQ
                     DFAVTATTTPDDLVNQVKSIVGDVPGLMSKPAAPTPTVPPTAP"
     gene            complement(825..1949)
                     /locus_tag="VGP27_15340"
     CDS             complement(825..1949)
                     /locus_tag="VGP27_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7852707.1"
                     /translation="MTESLPARPETSVSVRAVSAKPDLRDRLLKATGGPTLRAIARIP
                     DRIKRVLLGGRSVTIDGNTLDATLQLTLAAQRLSGRAGLILSTDVATARTRLNLTASQ
                     FPRTEVDVTSSDLQLPGPAGSIAARHYRPRNGGDAPLLVYYHGGGFVVGGLETHGNLC
                     EVICQNGGVHVLFVDYRLAPEHKAPAAVDDAYAAYLWAREHAAELGADSSRVAVGGDS
                     AGGNLAAVVAQRARNEGAPPAALQLLLYPITNFAGQTRSADLFADGLFLRRRDMDFCR
                     DKYLDDSGIDPTDPRVSPLLTDDLSGLPPAMLVTAGFDPLRDEGRQYAEALRAAGNSV
                     DAREFGSLIHSFPNFSTLGGGSAAATDEVISALRGHLSRS"
     gene            1948..2769
                     /locus_tag="VGP27_15345"
     CDS             1948..2769
                     /locus_tag="VGP27_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7852708.1"
                     /translation="MPSPSVKLNDGNSIPQVGLGVFQTPPDATEQAVATALDAGYRHI
                     DTAAVYGNEAETGRAIANSGVPRDDVFVTTKLWNADQGYQSTLAAFDASLDRLGIDRL
                     DLYLIHWPTPAKNTFVDTFKAFAHLHDQGRISSIGVSNFEPEHLRILIDATGIVPAVN
                     QVELNPRLQQEELRETHAALGIATEAWAPLGQGSLLSEPTVSAVADAHGKTPAQVLIR
                     WHIQLGNIVIPKSVTPERIVSNFDVFEFELTEQDMASISSLRDGTRLGPDPRNFN"
     gene            2796..3635
                     /locus_tag="VGP27_15350"
     CDS             2796..3635
                     /locus_tag="VGP27_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7852709.1"
                     /translation="MTSAAAIPSVTLIDERTMPVIGLGVGELSDSETEEAVSAALDAG
                     YRSIDTAAAYGNEAAVGRAVASSGVPREDLFITTKLATEAQGYQSSQDALAASLERLG
                     LDYVDLYLIHWPAGESGKYIDSWGGLLSLQGGGLTRSIGVANFHEEHLSNIIDLSFVT
                     PVVNQIELHPLLNQAALRVVNAGRGVVTEAYGPLGVGRLLDNPTIVSVAEAHGKTPAQ
                     VLIRWSIQLGNVVIPRSSSPERVRSNLDVFDFELMDDEMATINGLDDGTRFRPDPETY
                     TGT"
     gene            complement(3638..4348)
                     /locus_tag="VGP27_15355"
     CDS             complement(3638..4348)
                     /locus_tag="VGP27_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEV7852710.1"
                     /translation="MSRKRLAWLALAVTLAIMVAYQTVTSADRRAALFVAQADVPTVA
                     PGADVLAGVAQIPMRIHGFDYRRAAFGDSWTDDNSAPGGHNGCDTRNDILDRDLVDKT
                     YVSIKRCPNAVATGTLHDPYTNEAIAFVRGNQVGASVQIDHIVPLALAWDLGARNWPD
                     DVRLRFANDPANLLAVAGQANQDKGDSEPAVWMPANAAFACQYAIGFVAVLRGYGLPV
                     DAPSAVVLRDAASTCPTS"
     gene            complement(4345..>5022)
                     /locus_tag="VGP27_15360"
     CDS             complement(4345..>5022)
                     /locus_tag="VGP27_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV7852711.1"
                     /translation="GRQAYVVASRIDENDKKSNEKEDGPPATTVVELLERLRQGPLSG
                     LRLGLMHGRLSADEKDAAMAAFRAGEIDVLVCTTVIEVGVDVPNATVMFVMDADRFGI
                     SQLHQLRGRIGRGEHASLCLLATKLPEQSKAGQRLKAVADTLDGFELADLDLKERREG
                     DVLGYSQSGRPITLRFLSLFDHRDIIEAAREFCQAHYAQNPRDPGMALLAAEFTDTER
                     VEYLDKA"
BASE COUNT          851 a         1656 c         1671 g          844 t
ORIGIN      
        1 acggtcacgg cgccgtcggc gggacggtcg gagtcggcgc ggccggcttg ctcatcagcc
       61 cgggcacgtc gccgacgatc gacttgacct gattcaccaa gtcgtcgggt gtcgtggtcg
      121 ccgtgaccgc gaagtcctgg ccgttgatct tgatcgtcgg agtggactgg atctgggtcg
      181 cctgcgccag accctgcacc atcggcacgt atttgccctt gttgatgcag tccggcacgc
      241 tgccggccgc gcccgcctgg cgcgcgatct cgatcagccg ggcgttgtcg gggaagctcg
      301 ccgtcgtctc acccggctgc tgtgcgtaga gcgcggcgtg gaagcggcgg aacgcgtcct
      361 tgctctcgtc ggcgacgcag taggcggcag cgcccgctcg cgaggagtag ttgtcgttgc
      421 tcggctggtc gaggatggcg accatgtagt aatcggcggc gatcgctccg ctgtcgatga
      481 gctggccgat cgtcggaccg aacttctgct cgaattcacc gcagtgcggg cacaggaagt
      541 cctcgtagag cgacaccacg gccttgggct cggtggtgcc ctccttggtg atgagcttgc
      601 tcgacgacac cgtaatggcc ttggcctcgc cggacgcggg cttcttgtcg gcacccatca
      661 cgatgtagag caccagcccg acgccaagca ggaccacgac cgcggtcaaa ccgatctgaa
      721 tcagcaggtt gcgcctgcgg tcggcggctt tgaggtcgta acgggcgttc ttcgtgggtt
      781 tcgtggccac gggtcaaggg taccggcgag gttcctgcgg gtcgtcagct gcggctcaga
      841 tgcccgcgga gggcggagat gacttcgtcc gtcgccgccg cactgccacc gccgagggtg
      901 gagaagttgg gaaacgaatg gatcagcgag ccgaattcac gcgcatcgac ggagttgccg
      961 gctgcgcgca gtgcctcggc gtattggcgg ccctcgtcgc gaagcgggtc gaagccggcc
     1021 gtgaccagca tggcgggcgg caaaccggac aagtcgtcgg tcaacagcgg cgacaccctt
     1081 ggatcggtcg ggtcgatgcc cgaatcatcg aggtacttgt cgcggcagaa gtccatgtcc
     1141 cggcggcgaa ggaagaggcc gtcggcgaac aagtccgcgg accgggtctg gccggcgaag
     1201 ttggtgatcg gatacagcag cagctgaagg gcggccggcg gcgcgccttc gtttcgggcg
     1261 cgctgggcga ccaccgcggc gaggttgccg cccgcgctgt ccccgccgac tgcgacccgg
     1321 ctcgagtcgg cgccgagttc cgcggcatgc tcacgggccc acaggtacgc cgcgtacgcg
     1381 tcgtcgacgg ctgcgggcgc cttgtgctcg ggcgcgagtc ggtagtcgac gaacaacacg
     1441 tgcacgccgc cgttctggca gatcacttca cataggttgc cgtgggtttc cagaccgccg
     1501 acgacgaaac cgccgccgtg gtagtagacg agcagcggtg cgtcaccgcc gttgcgcgga
     1561 cggtagtggc gcgctgcgat gctcccggca ggccccggca actgcaggtc ggacgatgtg
     1621 acatccacct cggtccgggg aaactgcgac gcggtcaagt tcaggcgcgt gcgcgcggtc
     1681 gcgacgtcgg tgctgaggat caggcccgcc ctgccgctga gccgctgcgc ggccagcgtc
     1741 agctgcagcg tggcgtccag ggtgttcccg tcgatggtca ccgagcgccc acccaacagc
     1801 actcgcttga tccgatcggg gatgcgagcg atcgcgcgca acgtcggccc gccggtggcc
     1861 ttgagtagcc ggtcccgaag gtcgggtttc gccgagacgg cacgcacgct gacagaagtc
     1921 tcaggtcgcg ctggcagact ttcggtcatg ccttctccct ccgtaaagct gaacgacggt
     1981 aactcgatcc cacaggtcgg actcggcgta ttccagacgc cgccggatgc aaccgagcag
     2041 gcggtggcga ccgcgttgga cgcgggctac cgccatatcg acaccgccgc cgtctacggc
     2101 aacgaggccg agaccggcag agcgatcgcc aactcgggag ttccccgcga cgatgtcttc
     2161 gtcacgacga agctctggaa tgccgaccag ggataccaga gcacactggc cgcattcgac
     2221 gccagtctgg accggctcgg catcgaccgc ctcgacctgt acctcatcca ctggccgacg
     2281 cccgccaaga acaccttcgt cgacaccttc aaggcattcg cccacctgca cgaccagggg
     2341 cggatctcgt cgatcggggt gagcaatttc gagccggagc acctgcggat tctgatcgac
     2401 gcgaccggca tcgttcccgc cgtcaaccag gtggagttga atccacgcct gcagcaggag
     2461 gagctccggg agacacacgc tgcgctgggc atcgccaccg aggcgtgggc gccactgggt
     2521 cagggatcgc tgctgagcga gccgacggtg agcgcggtcg cggacgcgca cggaaaaacg
     2581 cctgcacagg tactgattag gtggcatatc cagctcggca atatagtcat cccgaagtcg
     2641 gtgactcccg agaggattgt gagcaacttc gacgtgttcg aattcgagct gaccgaacag
     2701 gacatggcgt ccatctcgtc gctgcgcgac ggaactcggc tcggtcccga cccacggaac
     2761 ttcaactaac cttcaactca gcagggatag gtgacatgac ctcggcggca gccattcctt
     2821 ccgtcactct gatcgacgaa cgcaccatgc cggtgatcgg cctgggagtc ggcgagctgt
     2881 ccgacagcga gaccgaagag gcggtgtccg cggcgttgga cgcgggctac cggtcgatcg
     2941 acaccgccgc cgcctacggc aacgaggccg cggtcggacg tgcggtcgcg tcttcgggcg
     3001 taccgcgcga ggatttgttc atcaccacca aactcgcaac agaagcccag gggtaccagt
     3061 catcacagga cgccctggcg gccagcctgg agcgcctcgg cctcgattac gtcgacctgt
     3121 atttgatcca ctggcccgcc ggtgagtcag gcaagtacat cgacagctgg ggtggactgc
     3181 tgtcactcca gggtggcggc ctgacccggt cgatcggcgt ggccaacttc cacgaagagc
     3241 acctgtcgaa catcatcgac ctgtcgttcg tcacccccgt cgtcaatcag atcgagctgc
     3301 acccgctgct caaccaggcc gcgctgcgcg tggtcaacgc cggccgaggg gtcgttacgg
     3361 aggcctacgg cccgttgggc gtcggcaggc tgctggacaa tccgacaatc gtctcggtcg
     3421 ccgaggcgca cggcaagaca ccggcgcagg tgctgatccg ctggagcatc cagctgggca
     3481 atgtggtgat tccgcgctcg tcgtcgccgg agcgggtgcg gtcgaacctc gacgtgttcg
     3541 atttcgagct gatggacgac gagatggcga cgatcaacgg gctcgacgac ggcacccgat
     3601 tccgcccgga tcccgaaacc tacaccggca cctgacctca gctcgtcggg caggtggacg
     3661 cggcgtcgcg cagcaccacc gccgacggcg catcgacggg caggccgtag ccgcgcagca
     3721 ccgcgacgaa cccaatggcg tactgacagg cgaacgcggc gttggccggc atccacaccg
     3781 caggctcgga atcacccttg tcctgattcg cctggcccgc caccgcaagc agattggccg
     3841 gatcgttggc gaagcgcagc cgcacatcgt cgggccagtt gcgggcgcca aggtcccatg
     3901 ccaacgccaa tggcacgatg tggtcgatct gcacggacgc gccgacctgg ttgccgcgca
     3961 cgaacgcgat cgcctcgttg gtatacggat cgtgcaatgt tccggtggcc acggcgttgg
     4021 gacaccgctt gatcgacacg taggtcttgt cgacgagatc gcggtcgagg atgtcgttgc
     4081 gggtgtcgca cccgttgtgg ccgccgggtg cggagttgtc gtctgtccac gagtcgccga
     4141 aagccgccct gcggtagtcg aatccgtgga tccgcatggg tatctgcgcc accccggcca
     4201 gcacatccgc gcccggagcg acggtgggaa cgtcggcctg cgcgacgaac aacgccgcac
     4261 gccggtccgc ggacgtgacc gtctgatagg cgaccatgat ggccagcgtc accgcaagcg
     4321 ccagccacgc cagccgcttg cggctcatgc cttgtccagg tattcgaccc gctcggtatc
     4381 ggtgaattcg gctgccagca gtgccattcc gggatcgcga ggattctgcg catagtgcgc
     4441 ttggcagaac tcgcgggccg cttcgatgat gtcgcggtgg tcgaaaagcg acaggaatcg
     4501 cagggtgatg ggcctaccgg attggctgta ccccaacacg tctccctcgc ggcgctcctt
     4561 gaggtcgagg tcggcgagct cgaagccgtc cagcgtgtcc gcgaccgcct tgagtcgttg
     4621 gcccgccttc gattgttccg gcagcttggt cgccagcaga cacaggctcg cgtgctcgcc
     4681 acgaccgatg cgaccccgca gctggtgcag ctggctgatg ccgaaccggt cggcatccat
     4741 cacgaacatg accgtcgcat tggggacgtc aacaccgacc tcgatgacgg tcgtgcacac
     4801 cagcacatcg atctcacccg cacggaacgc cgccatcgca gcgtccttct cgtcggcgga
     4861 cagccggccg tgcatcaatc cgagccgcag tcccgacagc ggtccttgcc gaagacgctc
     4921 gagcaactcc accaccgtgg tcgcaggcgg cccgtcctct ttctcgttgc ttttcttgtc
     4981 gttctcgtcg atgcgcgatg cgacgacgta ggcctgacga cc
//
LOCUS       DASYLX010000649        10960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2886794,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000649 DASYLX010000000
VERSION     DASYLX010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886794"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VGP27_15365"
     CDS             complement(<1..660)
                     /locus_tag="VGP27_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEV7852712.1"
                     /translation="MAIDVTVLRVFADADGKFGNPLGVVDASAVDPADRQRIAAELGY
                     SETIFIDLPEPGANTAHARIFTPATELPFAGHPTVGASWWLRDIGRPIHTLQVPAGVV
                     QVSYDADVAAVRARAEWAPDFAIYNLKSTDELFAAAPDDYPEDVENYLWTWTDEDNGV
                     LRSRMFAVALGVPEDEATGAAAVRITDYLSRDLTIVQGKGSVIRTQWSADGWVRVAGR
                     VV"
     gene            complement(697..1728)
                     /locus_tag="VGP27_15370"
     CDS             complement(697..1728)
                     /locus_tag="VGP27_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7852713.1"
                     /translation="MTARKSKKPNLRPVRELTPTLQYRTIHGYRRAYRVAGSGPAILL
                     IHGIGDNSTTWSSVQSTLAQRFTVIAPDLLGHGKSDKPRADYSVAAYANGMRDLLSVL
                     DIERVTVVGHSLGGGVAMQFAYQFPQLVDRLILVGAGGVTKDVNFALRIASLPMGSEA
                     LALLRLPLVLPTVQAVGRVAGLAIGKTGVGRDLPEMLRILRGLPEPTASSAFARTLRA
                     VVDWRGQVVTMLDRCYLTESVPVQLIWGSHDSVIPVSHARLAHSAMPGSRLEIFEGSG
                     HFPFHDDPDRFVEIVERFIDSTEPATYDPELLRGLLRDGIGEGTITGPVDTRVAVLDA
                     MGTDERSAT"
     gene            complement(1762..2700)
                     /locus_tag="VGP27_15375"
     CDS             complement(1762..2700)
                     /locus_tag="VGP27_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEV7852714.1"
                     /translation="MYELEFPAPQLSSTDGRGPVLIHALEGFSDAGHAIRLSAQHLKD
                     SLDTELVASFAIDELLDYRSRRPLMTFKTDHFTHYEEPELSLYALHDSVGAPFLLLAG
                     MEPDLRWERFITAVRLLAERLDVRQTIGLGTIPMAVPHTRPITLTAHANHKELIAGHT
                     PWVGEVQVPGSVSNLLEYRMAQHGHDVVGFTVHVPHYLAQTDYPAAAEALLTHVAKSG
                     SLQLPLGALAESAAEVHAKVEEQVGSSAEVAQVVEALERQYDAFIAAQENQSLLARDE
                     DLPSGDELGAEFERFLAQHAREKKRKEGFQDGDDGD"
     gene            2870..3649
                     /locus_tag="VGP27_15380"
     CDS             2870..3649
                     /locus_tag="VGP27_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7852715.1"
                     /translation="MRVSAVLVIGLLAAVAGCARPAPPAAHTATSETPSATKMSAAPV
                     PPDARIGALFLGAGDLHTCTAGVLDSADGDLILTAAHCVAEGVDTTFVPGFKDSADPA
                     AVWRVDTIYLDPRWVRNQDPLADFAILRVSRDGGGSVEAEAGGGLAIGTAPKPGTVVN
                     VTGYGLGVGGGPIGCESTTESAPGGFPSLPCGGLVDGTSGAPWTTGPTVTGLVGGLDG
                     GGCDENVSYSPPFDGGVGALLARAEEGGPGDEAPSVFDDDC"
     gene            complement(3651..5057)
                     /gene="sthA"
                     /locus_tag="VGP27_15385"
     CDS             complement(3651..5057)
                     /gene="sthA"
                     /locus_tag="VGP27_15385"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEV7852716.1"
                     /translation="MLEYDLVVIGSGPGGQKAAIAAAKLGKTVAVIERGRMLGGVCVN
                     TGTIPSKTLREAVVYLTGMSQRELYGASYRVKEKITPADLLARTTHVITKEQDVVRSQ
                     LMRNRVDLHTGHGRFVDAHTICVDDQTRGERTTVRGEFIVIATGTKPARPPGVEFDED
                     RVLDSDGILDLKSLPTSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDSMLDFCDPEV
                     VEALKFHLRDLAVTFRFGEEVTAVDVGAAGTVTTLASGKQIPAETVMYSAGRQGQTEH
                     LDAEKAGLEIDGRGRIFVDDQFQTKVDHIYAVGDVIGFPALAATSMDQGRLAAYHAFG
                     EPVRGMTELQPIGIYSIPEVSYVGATEVDLTRDSIPYEVGVSRYRELARGQIAGDSYG
                     MLKLLVSTEDLKLLGVHIFGTSATELVHIGQAVMGCGGTVEYLVDAVFNYPTFSEAYK
                     VAALDVMNKLRALSQFRS"
     gene            5208..5939
                     /locus_tag="VGP27_15390"
     CDS             5208..5939
                     /locus_tag="VGP27_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852717.1"
                     /translation="MVGTVLRALRRCTVPVVCCAVLTSAAVVAGCARVVSGTARAGDP
                     RPPPARPIPITDLLIDPTRFPAQYPAAVLPQEDVDRVLGEIDGVPVGSEVTPPECAPL
                     PVLAQQKAAMQGIDEDSGTRLTVTVIRPVPSVRARATQLAECPSFTSTSGGQAGEVSD
                     VTVDLPPAPPVDADDSYAVDQTVMSRSSAESDTRTLTLVALVDDVRVTVSWQQQASSE
                     ASPDTQTLDALFTDAVLRVRREIPR"
     gene            6034..7059
                     /locus_tag="VGP27_15395"
     CDS             6034..7059
                     /locus_tag="VGP27_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEV7852718.1"
                     /translation="MTTSQSDFQLNRPGALIAALPAVLGFVPEKSLVLVTVDRGELGC
                     VMRVDLSPGLAGSVGHLAEVAAAARPDAAIAVVVDAEGAGCRLCNDEYQDLHRGLAEG
                     LAENAIDLLDFHVVDTVGPGGRWHCLDGSWGAVDDPSASPLAMAAVLDGRRLYARREE
                     LLKVIEVTDAAASDALARVIRDQADVEVDEVRDIEDAMSAAVRVAAGQQLSEAVTARL
                     ACALTEPRVRDVLYALAVGDRAAQAEALWAVLAQTLPPPWRAEALVLLAFSAYARGDG
                     PLAGVSLEAALRCDATHRMAGMLDTALQSGLRPEQIRELAVTGYRLAKRLGVQLPRPT
                     QFGRRAG"
     gene            complement(7090..7782)
                     /locus_tag="VGP27_15400"
     CDS             complement(7090..7782)
                     /locus_tag="VGP27_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HEV7852719.1"
                     /translation="MNDLVDTTEMYLRTIYDLEEEGVVPLRARIAERLEQSGPTVSQT
                     VSRMERDGLLHVAGDRHLELTDKGRGLAIAVMRKHRLAERLLVDVIGLPWEEVHAEAC
                     RWEHVMSEDVERRLVQVLDNPTVSPFGNPIPGLFELGVGDSFGADDRNLVRLTELPAG
                     FPVAVVVRQLTEHVQGDVNLIARLKDAGVVPNARVTVEPGTNGGVKIVIPGHEQVELP
                     HEMAHAVKVEKV"
     gene            complement(7904..8893)
                     /locus_tag="VGP27_15405"
     CDS             complement(7904..8893)
                     /locus_tag="VGP27_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003902342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7852720.1"
                     /translation="MTVRAEQEAPMANATFSRFDQDLDAQSPAADLVRVYLNGIGKTA
                     LLNAADEVELAKRIEAGLYAQHLLDTRKRLGENRKRDLAIVVRDGQAARSHLLEANLR
                     LVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGM
                     ADQSRTIRLPVHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPVEKITDLLEHS
                     RDPVSLDMPVGSDEEAPLGDFIEDAEAMSAENAVISELLHTDIRHVLATLDEREQQVI
                     RLRFGLDDGQPRTLDQIGKLFGLSRERVRQIEREVMAKLRNGERADRLRSYAS"
     gene            complement(9011..9409)
                     /locus_tag="VGP27_15410"
     CDS             complement(9011..9409)
                     /locus_tag="VGP27_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEV7852721.1"
                     /translation="MKHGPQLGFDDDGRPVLITAAAPAFEEQHRARVRKYLTIMSFRI
                     PALILAAVAYGVWGNGLISLAILVASIPLPWIAVLIANDRPPRRAEEPRRYAARRIPL
                     FPTAERPALERGPRSAAQPEDVDGTGDVPS"
     gene            9487..9723
                     /locus_tag="VGP27_15415"
     CDS             9487..9723
                     /locus_tag="VGP27_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEV7852722.1"
                     /translation="MQTQTIERTDTDERVDDGTDSDTPKFFHYVKKDKIAESAVTGTH
                     VVALCGEVFPVTKAAKPGSPVCPDCKRIYEQLKK"
     gene            complement(9713..10711)
                     /locus_tag="VGP27_15420"
     CDS             complement(9713..10711)
                     /locus_tag="VGP27_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV7852723.1"
                     /translation="MNDHPAVKPSKHHIWRIAKRTLAKSWDDSIFSESAQAAFWCALS
                     LPPLLLGMLGSLAYIAPLFGPETLPMIQEQLINTANSFFSKNVVSEIIEPTIRDIVKG
                     ARGEVVSLGFVISLWAGSSAVSAFVDSITEAHDQTPLRHPVRQRLFALGLYVVMLVLA
                     IATAPIVALGPLKIGEHIPQSWDNFLRYGYYPALFLGLVVSVTFLYRVSLPKPLPTHR
                     LVFGAVLATTVFLVATVGLRIYLTYITSTGYTYGALATPIAFLLFAFFLGFAIMIGAE
                     LNAAVQEEWPAPATHAKRLRWWLQEKAAESLNGGTAPAAAPAAATPKGPSSSAPTS"
     gene            complement(10769..>10960)
                     /locus_tag="VGP27_15425"
     CDS             complement(10769..>10960)
                     /locus_tag="VGP27_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852724.1"
                     /translation="TLTSPELTRADRCDRCGAAARVRATLPSGAELLFCQHHANEHEA
                     KLVEMAAVIEVSPGEPVGA"
BASE COUNT         1772 a         3585 c         3733 g         1870 t
ORIGIN      
        1 caccacgcgg cctgcgaccc gaacccaccc gtcggcgctc cactgcgtcc ggatgaccga
       61 gcccttgccc tgcacgatgg tgaggtcgcg gctgaggtag tcggtgatgc gaaccgccgc
      121 cgcgccggtt gcctcgtcct ctggcacgcc gagggcgacc gcgaacatcc gggagcgcag
      181 cacgccgttg tcctcgtcgg tccacgtcca cagatagttc tcgacgtcct cgggatagtc
      241 gtcgggcgcg gcggcgaaga gctcgtcggt ggacttcagg ttgtagatcg cgaagtccgg
      301 tgcccattcg gcgcgggccc gcacagctgc gacgtcggcg tcgtaactga cctgaaccac
      361 cccggcgggc acctgcaggg tgtgaatcgg gcggccgata tcgcgcagcc accacgacgc
      421 tcctacggtc ggatgaccgg cgaagggcag ttcagtggcg ggtgtgaaga tccgggcatg
      481 cgccgtattc gcgccgggct ccggtagatc gatgaatatt gtttcgctgt aacctaattc
      541 ggcggcgatg cgctgccgat cggcggggtc gacggcgctt gcgtcgacga cgccgagtgg
      601 gttgccgaac ttaccgtccg catcggcgaa cacccgcagc actgtcacat cgatggccat
      661 gcgcccgatg ctacgacgtg acgaagaggg gcgggctcag gtggcgctgc gctcgtcggt
      721 gcccattgca tcgagcaccg cgacacgggt atcgaccggt ccggtgatcg tgccctcgcc
      781 gatgccgtcg cgcagcaggc cgcgcagcaa ttccgggtcg taggtggccg gttcggtcga
      841 gtcgatgaac cgctccacga tctcgacgaa gcggtccgga tcgtcgtgga acgggaaatg
      901 tccggagccc tcgaagattt ccagccgcga gccgggcatc gcggagtgtg ccagccgcgc
      961 gtggctgacc ggtatcaccg aatcgtgaga gccccaaatc aactgaacgg gaacggattc
     1021 cgtcaaataa catcggtcca gcatggtgac cacctgcccg cgccagtcga cgacggcccg
     1081 cagcgtgcgg gcgaacgccg aggaggcggt cggctcgggt aggccgcgga gaatgcgcag
     1141 catctcggga aggtcgcggc caaccccggt cttgccgatc gccaggcctg cgacccgccc
     1201 caccgcctgc accgtgggaa ggaccagcgg cagccgcagc agcgccagcg cttcactgcc
     1261 catcggcagg gacgcgatcc gaagcgcgaa gttcacgtcc ttggtcactc cgcccgcgcc
     1321 gaccaggatc agtcggtcca ccagttgcgg gaactgatag gcgaactgca tcgcgacgcc
     1381 accaccgagc gaatgaccaa cgaccgtgac ccgttcgatg tcgaggacgc tgagcagatc
     1441 ccgcatcccg ttcgcgtacg ccgccacgga gtagtcggcg cggggtttgt cggactttcc
     1501 gtgcccgagc aagtcagggg cgatgacggt gaatcgctgg gcgagcgtgg attgcacgct
     1561 gctccacgtc gtcgagttgt ccccgatccc gtggatcagc agtatcgcgg gacccgatcc
     1621 ggcgacccgg tacgcccggc ggtagccgtg gatggtgcgg tattgaagcg tgggcgtcag
     1681 ctcccgcacc ggacggagat tgggcttttt ggactttcga gctgtcatgt ggccaacctc
     1741 gtcgtcggac cgggtgatgg cctaatcgcc gtcgtccccg tcttggaagc cttcttttct
     1801 cttcttctcc cgcgcgtgct gtgcgagaaa tcgctcgaac tcggcaccga gctcgtcacc
     1861 gctgggcaga tcctcgtcgc gggccagcag tgattggttc tcctgggctg cgatgaacgc
     1921 atcgtactgg cgctcaaggg cctccaccac ttgggcgact tcggcgctcg agcccacctg
     1981 ttcctcgact ttcgcgtgca cttcggcggc cgattcggcc agggcgccca gcggcagctg
     2041 caacgagccg gacttggcca cgtgggtgag cagcgcctcg gcggccgccg ggtagtccgt
     2101 ctgcgcgagg tagtgcggca catgcacggt gaaccccacc acgtcgtggc cgtgctgcgc
     2161 catccggtac tcgagcaggt tggacacact gccgggaacc tgcacctcgc cgacccacgg
     2221 ggtgtgaccg gcgatcagct ccttgtggtt ggcgtgggcg gtcagcgtga tcgggcgggt
     2281 gtgcggcacc gccatcggaa tggtgcccag cccgatggtc tgccggacgt cgagccgttc
     2341 ggccagcagc cgcaccgcgg tgatgaaccg ttcccagcgc aggtccggct ccatgcccgc
     2401 gagcagaagg aacggggctc cgacgctgtc gtgcagggcg tagaggctca gctcaggttc
     2461 ctcgtagtgg gtgaaatggt cggtcttgaa cgtcatcagg ggccgccgcg accggtagtc
     2521 cagcagttcg tctatggcga acgacgcgac cagctcggtg tccaatgagt ccttgaggtg
     2581 ctgggcggac aaccggatgg cgtgcccggc gtcggagaac ccctccaggg cgtggatcag
     2641 caccgggccg cggccgtcgg tggacgacaa ctgcggcgcg gggaactcca gctcgtacat
     2701 cccgctctgg tcgggttcgt accgctgttc ctgcttgtcg ttctcgtcgc tgattgcgct
     2761 cgcctcctcg acaccgccac ttccagtgtc tcatctgtcc ccaacgcggg cctcgaagtc
     2821 ctcatgccgc gccgccgtgg acgccggcga tcgggcaaga tcagacttta tgcgggtatc
     2881 ggcggtgctg gtgatcgggc tgctggcggc tgtggccgga tgcgcgcggc ccgcgccccc
     2941 tgcggcccac acggcgacga gtgagacgcc gtcggcgacg aagatgtcgg ctgctcccgt
     3001 gccgccggac gcacggatcg gcgcgctgtt cctcggtgcg ggcgatctgc acacgtgcac
     3061 cgcgggcgtc ctcgactcgg cggacggtga cctcatcctc accgctgcgc actgcgtcgc
     3121 cgagggcgtc gacaccacgt tcgtccccgg tttcaaggac tccgccgacc cggcggcggt
     3181 ctggcgggtc gacacgatat acctcgaccc tcgctgggtg cggaaccagg atccgttggc
     3241 ggacttcgcg attctgcgag tcagccgtga cggcggcggg tcggtggagg ccgaggccgg
     3301 gggtggcctc gcgatcggca ccgcaccgaa gccgggcacg gtggtcaacg tgaccgggta
     3361 cgggttgggt gtcggaggcg gtccgatcgg ctgcgagtcg acaaccgagt cggcgccggg
     3421 cggcttcccg tcgctgccgt gcggcgggct tgtcgacgga accagtggcg ccccgtggac
     3481 cacgggaccg accgtcaccg gtttggtcgg tgggcttgac ggtggtggat gtgacgaaaa
     3541 cgtctcgtac tcaccgccgt tcgacggcgg ggtgggcgcg ctgctggcgc gcgcagagga
     3601 aggcggtccg ggcgacgaag cgccctcggt gttcgacgac gactgctgat ctagctgcgg
     3661 aactggctga gcgcgcgcag cttgttcatc acgtccagcg ctgccacctt gtacgcctcg
     3721 gagaaggtcg ggtagttgaa gacggcgtcg acgaggtatt cgacggtgcc tccgcagccc
     3781 atcacggcct ggccgatgtg caccagctcc gtggcactgg taccgaagat gtggacgccc
     3841 agcagcttca gatcctcggt cgagacaagc agtttgagca ttccgtagga gtcgcccgcg
     3901 atctggccgc gggccaactc gcgataccgg gacacgccga cctcgtaggg gatggagtct
     3961 cgggtcaggt ccacttccgt ggcgccgaca taggacacct cggggatgga gtagatgccg
     4021 atcggctgca gttcggtcat gcctctcacc ggctcgccga acgcgtggta ggccgccagc
     4081 ctgccctggt ccatcgaggt ggcggccagc gccgggaagc cgatcacatc gccgacggcg
     4141 tagatgtggt caaccttcgt ctggaactgg tcgtcgacga agatccgacc gcgtccgtcg
     4201 atctccagcc cggccttctc ggcgtcgagg tgttcggtct ggccctggcg gcccgccgag
     4261 tacatcaccg tctccgcggg gatctgcttg ccgctggcca gcgtcgtgac ggtgcccgcg
     4321 gcgccgacgt cgacggccgt cacctcctcg ccgaagcgga acgtcaccgc caggtcgcga
     4381 aggtggaact tcagtgcctc gacgacttcg gggtcgcaga agtccagcat gctgtcgcgc
     4441 ttctccacca ccgtgacctt ggtgcctagc gcggcgaaca tcgacgcgta ttcgatgccg
     4501 atcacgccgg cgccgaccac caccatcgat gtcggaaggg acttcagatc caggatgccg
     4561 tccgagtcga gcacccggtc ctcgtcgaat tcgacgcccg gcggacgggc gggcttggtg
     4621 cccgtggcga tgacgatgaa ttcgcctcgg acggttgtcc tttcgccgcg ggtctgatca
     4681 tcgacacata tggtgtgcgc gtcgacgaac cggccgtgac cggtgtgcag gtcgacccgg
     4741 ttgcgcatca gctggctgcg caccacgtcc tgctccttgg tgatgacgtg tgtggtgcgg
     4801 gccaggaggt cggcgggcgt gatcttctcc ttcacccggt agctggcgcc gtagagctct
     4861 cgctggctca tcccggtcag gtagaccacc gcttcgcgca gcgtcttgga ggggatggtt
     4921 ccggtgttca cacacacgcc gccgagcatc cggccgcgtt cgatgacggc gaccgtcttg
     4981 ccgagctttg ccgccgcgat ggcggccttc tgcccaccgg gacccgaacc tatgacgacg
     5041 aggtcgtact ccagcaccga agccatatgt gaaggtgtac gccgattgcg gggatctcgc
     5101 atgccgacgc ggtcaatacc gggtgaacaa cacctgcgga ccggctgacg taccggccac
     5161 cgcgcgcctg agtgcgccgg gcacaggccg atgagacgat gacgacaatg gtcggcactg
     5221 ttctgcgggc tctacgccgg tgcacggtgc ccgtcgtctg ctgcgccgtg ctgacgtcgg
     5281 ctgcggtggt ggccggctgc gcacgcgtgg tcagcggcac cgcgcgggcc ggcgatccgc
     5341 ggccaccccc ggcccgaccg atacccataa ccgacttgct catcgatccg acacggttcc
     5401 ccgcgcagta tccggcggcc gtactgcccc aggaggacgt cgaccgcgtg ctcggcgaaa
     5461 tcgacggcgt accggtcgga tccgaggtga cgccgcccga gtgcgcgccg ctgccggtac
     5521 tcgcgcagca gaaggccgcc atgcagggaa tcgacgagga cagcggtacc cgcctcaccg
     5581 tcaccgtcat ccgcccggtg ccctcggtgc gcgccagggc cacccagctc gccgaatgcc
     5641 catcgttcac aagcacatcg ggcgggcagg ccggggaggt gtcggacgtg acggtggacc
     5701 tgccgcctgc accgccggtg gacgccgacg acagctacgc ggtcgaccag accgtcatgt
     5761 cgaggtcttc cgccgagtcc gacaccagga ccctgaccct ggtcgccctc gtcgacgacg
     5821 tccgggtgac ggtgtcgtgg cagcagcagg cgtcatcaga ggcgtcaccg gacacccaga
     5881 cgctggacgc gctgttcacc gatgcggtgc tcagagtgcg ccgggagatt ccgcgctagc
     5941 cggctatacc cagtcgcgcc ttcatccaca gctgcaccgc ctccgcggtc cgccgtggat
     6001 cgcgcagacg gtggcgcccg tcagtttcta gacatgacga catcgcaatc cgatttccag
     6061 ctcaacagac ccggcgcgct gatcgcggcg ctacccgccg ttctcggctt cgtgcccgag
     6121 aagtccctcg ttctggtgac cgtcgaccgc ggggagttgg gttgcgtcat gcgcgttgac
     6181 ctttcgcccg gattggccgg ctcggtcggg caccttgccg aggtcgccgc cgcggcccga
     6241 ccggacgccg cgatcgcggt cgtggtcgac gccgagggcg ccggctgccg gttgtgcaac
     6301 gacgaatacc aggatctgca ccgcggactg gccgagggcc tggccgagaa tgcgatagac
     6361 ctgctcgact tccacgtcgt cgacacggtc ggaccgggcg gccggtggca ttgtctggac
     6421 ggcagctggg gcgccgtcga cgacccgtcg gcgtcgccgc tggcgatggc ggcggtactg
     6481 gacggtcgca ggttgtacgc ccggcgcgag gagctcctca aggtcatcga ggtgaccgat
     6541 gcggcggcca gcgacgcgct ggcccgcgtg atccgcgatc aggctgatgt cgaggtcgac
     6601 gaggtgcgtg acatcgagga cgcgatgtcg gctgcggtgc gggttgctgc gggacaacag
     6661 ctgtcagagg ccgtgacggc ccggctggcc tgtgcgctga ccgaaccgcg ggtgcgcgat
     6721 gtgctctacg cgctggcggt gggggacagg gccgcacaag cggaagcgct gtgggcggtg
     6781 ctggcacaaa cacttccgcc gccgtggcgg gccgaggcgc tggtgctgtt ggcgttctcc
     6841 gcctacgccc gcggagacgg gccgctggcc ggggtgtcgc tggaggcggc gctgcgttgc
     6901 gacgccacgc accggatggc gggcatgctg gataccgcac tgcagtcggg gctgcggcca
     6961 gaacagatcc gcgagctcgc cgtcacggga taccggctgg ccaagcggct cggagtgcag
     7021 ttgccgaggc cgacgcaatt cggtcggcgc gcgggctaga tccgttgcgg cgcaggcctt
     7081 ccggcccctc tagaccttct cgaccttcac ggcgtgggcc atctcgtgtg gaagctcgac
     7141 ctgctcgtgg cccgggatca cgatcttgac gccgccattg gtgccgggtt cgacggtcac
     7201 ccgtgcgttg ggaaccacgc ctgcgtcctt gagccgggcg atcaggttga cgtcaccctg
     7261 gacgtgctcg gtcagctggc gcacgacgac agccaccggg aagccggcgg gcagctcggt
     7321 cagccgcacc agattgcggt catcggcgcc gaaggagtcg ccgacgccca gctcgaaaag
     7381 gccggggatg ggattgccga acggggaaac ggtcgggttg tcgagcacct gcacgaggcg
     7441 ccgctcgacg tcctcgctca tcacgtgctc ccagcggcac gcctcggcgt gtacttcctc
     7501 ccacggcagc ccgatcacgt cgaccagcag acgctcggcc agccggtgct tgcgcatgac
     7561 ggcgatggcc agtccgcggc ccttgtcggt cagctccaga tgccggtctc cggcgacatg
     7621 cagcaggccg tcgcgctcca tccgggagac ggtctggctc accgtcggtc cgctctgctc
     7681 gagacgctcg gcgatgcgcg cacgcagagg caccacgccc tcttcttcga ggtcgtagat
     7741 ggtccgcagg tacatctcgg tggtgtcgac cagatcgttc atgggaaacc cttcgtctgg
     7801 catgagtcta ccggcctagc tgcacgaatc atgcttctac gttgtcaggg tgtagcaaga
     7861 aggcccgccg gtgttgaccg gcgggccttc ttgctgggga gcgctagctg gcgtaggagc
     7921 gcaagcgatc ggcgcgttcg ccgttgcgca gtttggccat cacctcgcgc tcgatctggc
     7981 gcacccgttc ccgggacaac ccgaagagct tgccgatctg atcgagcgtg cggggctggc
     8041 cgtcgtcgag accgaaccga agccggatca cctgctgctc acgctcatcg agcgtggcca
     8101 gcacatggcg aatatcggtg tgcagcagtt cggagatcac ggcgttttcg gccgacatcg
     8161 cctcggcgtc ctcgatgaag tcacccagag gggcttcctc gtcgctgccg acgggcatgt
     8221 cgaggctcac cgggtcacgg ctgtgctcga gcagatcggt gatcttctcc accggaatgc
     8281 ccgactcggc ggccagctcc tcgtcggtgg cttcacggcc gaggttctgg tgcatttccc
     8341 gcttgatccg ggcgagcttg ttcacctgct cgacgagatg gacgggcagc cggatggtgc
     8401 ggctctgatc ggccataccg cgggtaatcg cctgacgaat ccaccacgtg gcataggtcg
     8461 agaacttgaa acccttgctg tagtcgaact tctccatggc gcggatcaga ccgaggttgc
     8521 cctcctgaat caggtccagc agcggcatgc cacggccggt gtatcgcttc gccagcgaaa
     8581 ccaccagccg caggttggcc tcgagcagat ggctgcgggc cgcctggccg tcgcgcacga
     8641 cgatcgccag atcgcgtttg cggttctctc cgagacgctt gcgggtgtcc agcagatgtt
     8701 gtgcgtaaag cccggcctcg atccgcttcg cgagttcaac ctcgtcggcg gcgttgagca
     8761 aagccgtctt gccaatgccg ttcaaataca cgcgcacaag atcagcggct ggactctgag
     8821 cgtccagatc ctggtcgaag cggctgaatg tggcattcgc catcggggcc tcctgctcgg
     8881 ctcgaactgt caagaacttc aacgcccaat cgcaccgaag agttcccacg aaccggccgg
     8941 ttcacacgtg ttgatcagcg gttttggccc gatggcctga gaatcgtctg agaatacgac
     9001 tagacgccgc tcaggaagga acgtcgcccg tcccgtcgac gtcttcgggt tgtgcggccg
     9061 accgcggtcc ccgctccagg gccggccgct cggcggtcgg gaacaacggt atgcggcggg
     9121 cggcgtagcg ccgcggctcc tctgcgcggc gcggtgggcg gtcgttcgcg atgagtaccg
     9181 cgatccacgg cagcggtatc gacgccacga gaatcgccag cgagatgagc ccgtttcccc
     9241 acacgccgta ggccaccgcg gcgaggatca gggccgggat gcggaacgac atgatggtca
     9301 ggtacttgcg cacccgtgca cgatgttgtt cttcgaaggc gggtgcggcg gcggtgatga
     9361 ggacgggacg tccgtcgtcg tcgaaaccca gctgtgggcc gtgtttcata tgtcccactg
     9421 ttccacattc atggatcgac gaaccgatcg gtttcttacg gatctattgc cgggcacaat
     9481 gaaatcatgc aaacccagac gatcgagcgc accgacaccg acgaacgcgt cgacgacggg
     9541 actgacagcg acaccccgaa gttcttccac tacgtcaaga aggacaagat cgccgaaagc
     9601 gccgtcacgg gcacgcatgt ggtcgcgttg tgcggtgagg tctttcccgt cacgaaggca
     9661 gccaagccgg ggtcgccggt gtgcccggac tgcaagcgga tctacgagca gctcaagaag
     9721 taggggctga agaagacggg cccttcggcg tcgcggccgc aggtgccgcc gccggggcgg
     9781 tacccccgtt gagcgactcg gcggccttct cctgcagcca ccaccggagc cgcttggcat
     9841 gtgtggccgg agcgggccac tcctcttgca ccgcggcgtt gagctcggca ccgatcatga
     9901 tcgcgaagcc gaggaagaag gcgaacagca ggaacgcgat cggcgtcgcc agcgcgccgt
     9961 aggtgtaacc ggtgctggtg atgtaggtca gatagatgcg cagccccacc gtggcgacga
    10021 ggaacaccgt tgtggccagg actgcgccga acaccagacg atgcgtgggc aacggcttgg
    10081 gcagtgacac ccgatagagg aacgtcaccg atacgaccag gcccagaaac aacgccgggt
    10141 agtagccgta gcgcagaaag ttgtcccagc tctgcggaat gtgctcgccg atcttgagtg
    10201 gcccgagcgc gacgatcggc gccgtcgcga tcgccagcac cagcatcacc acatagagtc
    10261 cgagcgcaaa gaggcgctgc cgtacgggat gtcgcaatgg ggtctggtca tgtgcttcgg
    10321 tgatcgagtc gacgaacgcc gagactgccg acgagccggc ccacagcgag atgacgaacc
    10381 cgagcgacac cacctcaccg cgggctccct tgacgatgtc acggatggtc ggctcgatga
    10441 tctcgctgac gacgttcttg gagaagaagc tgttggccgt attgatcaac tgctcttgga
    10501 tcatcggcag tgtttcgggg ccgaacaatg gcgcgatgta ggccaggctg ccgagcatcc
    10561 ccagcagcag gggcggcaac gacaacgcac accagaaggc ggcctgcgcc gactccgaga
    10621 aaatcgagtc atcccagctt ttcgccaggg tccgttttgc gatgcgccag atgtggtgct
    10681 tggacggctt caccgcaggg tggtcattca tgaccagtcc agcatccccg acgaagcgtc
    10741 gtcctgccca gactcgaagg tgtgaggatt acgcgccgac gggctctccc gggctgactt
    10801 cgatcacggc ggccatctcg accagcttgg cttcgtgctc attggcgtgg tgctgacaaa
    10861 acaacagctc ggctcccgac ggcaacgttg ctcgtacgcg agcagcggcg ccgcagcgat
    10921 cgcagcgatc agcccgggtc agttcgggac tggtcagagt
//
LOCUS       DASYLX010000650         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3435667,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000650 DASYLX010000000
VERSION     DASYLX010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3435667"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..857
                     /locus_tag="VGP27_15430"
     CDS             84..857
                     /locus_tag="VGP27_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-degrading phosphodiesterase"
                     /protein_id="HEV7852725.1"
                     /translation="MNVRITVLGCSGSVVGPDSPASGYLLTAPETPPLAIDFGGGVLG
                     ALQRHADPNAVHVLLSHLHADHCLDLPGLFVWRRYHPSPAPERGLMYGPKKTWARLGA
                     ASSPEGAEIDDFSDIFDIRHWVDREQVQIGSLTIEPRLVCHPTESYGLRVTDPSGATF
                     VYSGDTGACDSLVDLARGADVFLCEASWTHDGSRPPNLHLSGTEAGQAAARAGVGELL
                     LTHIPPWTSREDVISEAKAEFDGPVHAVVCGESFPVTRH"
     gene            886..1668
                     /gene="rph"
                     /locus_tag="VGP27_15435"
     CDS             886..1668
                     /gene="rph"
                     /locus_tag="VGP27_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV7852726.1"
                     /translation="MSRREDGRLDDELRPVVITRGFTSHPAGSVLVEFGQTRVMCTAS
                     VTEGVPRWRRGSGQGWLTAEYAMLPAATHERSDRESVRGRIGGRTQEISRLIGRSLRA
                     CIDLSALGENTIALDCDVLQADGGTRTAAITGAYVALADAVTWLAAAGKLADPRPLSC
                     AIAAVSVGVVDGRVRTDLPYTEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLD
                     KLLDAALAACEQLFVIQREALEKPYPGELPESDTPQKKAFGS"
     gene            1683..>1877
                     /locus_tag="VGP27_15440"
     CDS             1683..>1877
                     /locus_tag="VGP27_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEV7852727.1"
                     /translation="MQPTVELLVASRNHKKLAELRRVLDAAGISGLTLLSLDDVAPFD
                     EAPETGATFEDNAVAKARDAF"
BASE COUNT          302 a          601 c          662 g          312 t
ORIGIN      
        1 acgtcacgtc agcagccaat gatgacgtta gtcaatcaaa gcgctgatgt tttcgtcata
       61 gggatagcgg gcatggcaag ctagtgaatg tgcgaatcac cgtactcggt tgctccggca
      121 gtgttgtcgg gcctgattcg ccagcgtccg gataccttct gacggcgccc gaaaccccgc
      181 ccctggccat tgacttcggt ggtggtgtgc tcggtgcgct gcagcgccac gcggatccca
      241 acgcggtaca cgttttgctg tcgcacctgc acgccgacca ttgcctcgac ctccccggtc
      301 tcttcgtttg gcggcgctac cacccgtcgc cggcgcccga gcgtggcctg atgtacggac
      361 cgaagaaaac gtgggcccgc ctcggtgcgg cgtcgtcgcc cgagggcgcg gagatcgacg
      421 acttcagcga tatcttcgac atccggcact gggtcgacag agaacaggtg cagatcgggt
      481 cgctgacgat cgagccgagg ctggtgtgcc atccgacaga gtcctacggg ctgcgagtca
      541 ccgacccgtc cggcgcgacg ttcgtctaca gcggtgacac cggcgcgtgc gattcgctgg
      601 tcgatctggc ccgcggtgcg gacgtgttcc tctgcgaggc gtcgtggacg catgatgggt
      661 cgcgtccgcc gaacctgcac ctgtcgggca ccgaggccgg ccaggccgcg gcgcgcgccg
      721 gggtcggcga gttgctgctg acgcatattc caccgtggac gtcgcgcgag gacgtgatca
      781 gcgaggcgaa ggccgaattc gacggacccg tgcacgcggt ggtgtgtgga gagtcgttcc
      841 ccgtcacccg gcattgatga ccggagcacc ggctagggtt ggcgggtgtc cagacgagaa
      901 gacggccggc tcgacgacga gctgcgaccg gtggtcatca cccgcggttt cacctctcac
      961 ccggcgggat cggtactcgt cgagttcggt cagacccggg tcatgtgcac ggcaagcgtc
     1021 accgagggtg tgccgcgatg gcgcaggggt tcgggccagg gctggctgac tgcggagtac
     1081 gcgatgctgc ccgcggccac ccatgaacgc tccgaccggg aatccgtccg cggccgcatc
     1141 ggcggccgca cccaagagat cagccggctg atcgggcggt cactgcgcgc gtgtatcgac
     1201 ctgtcggcgc tgggggagaa caccatcgcg ctcgactgcg acgtgttgca ggccgacggc
     1261 ggcacccgca ccgccgccat cacgggtgcg tacgtggcct tggccgatgc ggtgacgtgg
     1321 ttggcggcgg ccggcaagct ggctgacccg cgtccgctgt cctgcgcgat cgccgcggtg
     1381 agcgtcggcg tggtcgacgg acgcgtccgc accgacctgc cgtacaccga ggactcgcga
     1441 gccgaggtgg acatgaacgt ggtggccacc gacaccggca cgctggtgga gatccagggc
     1501 acgggcgaag gtgcgacgtt cccgcgctcg acgctggaca agttgctcga cgccgcgtta
     1561 gcagcgtgcg agcagctgtt cgtgattcag cgcgaggctc tggagaagcc gtatcccgga
     1621 gagcttcccg agtcggatac cccccagaag aaggcgttcg gaagctgacg cgcacgtgcg
     1681 gagtgcaacc aaccgtggag ctgctggtgg ccagccgtaa ccacaagaag ctggccgaac
     1741 tgcgacgggt gctcgatgcc gccggaatct ctggtctgac gttgttgtcg ctcgacgatg
     1801 tcgccccgtt cgacgaggcg ccggagacgg gcgcgacctt cgaagacaac gcggtcgcca
     1861 aggcgcgcga cgcgttc
//
LOCUS       DASYLX010000651         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1680001,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000651 DASYLX010000000
VERSION     DASYLX010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680001"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VGP27_15445"
     CDS             <1..972
                     /locus_tag="VGP27_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852728.1"
                     /translation="LVDSASRINTAAQTVEFASGRRPVRYDYVIYAVGSTGAVPASVP
                     GATEFAYPIAELELAQRLCTAIDELHPDAPVTVIGAGLTGIEVASELAEQSRNVTLVC
                     GGQLAPSLSEPGRRSVAKWMHKHGVTVLESDVVAEVRRGAVVFADGAVRPSAITIWTA
                     GFGVPALAADSGLRTDALGRLLTDETLTSVDDARIVAAGDCAAPSGRPLRMSCQAAGP
                     LATQAADTVLSLIAGEEPAVIEPRFVAQCVSLGRHGATLQFTHEDDAPASFYLGGRVA
                     ASIKEAVCRGTLWGLRREARKPGSAFLFKGSKREDEPAVDAQVVTNP"
     gene            969..1907
                     /gene="sigJ"
                     /locus_tag="VGP27_15450"
     CDS             969..1907
                     /gene="sigJ"
                     /locus_tag="VGP27_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEV7852729.1"
                     /translation="MTTWGGNQIAGSAGGEHAERFTHLRPLLFTIVYEILGSATESDD
                     VLQDSYLRWAEVDLATVRDTKSYLAQLVTRQALNALRAGARRREEYIGPWLPEPLLLD
                     DRDASTDVVLAESVSMAMLVLLETLTPDERAVFVLREVFGFDYDEIAGAVGKSAVNVR
                     QVAHRAREHVQARRKRFEPADSAKTAELTEQFMTAASTGDVDGLMALLAPDATWTADS
                     NGKATAARRPVVGARKVAAILSALFRVAAERMPDVRFETAVYNSSPSIVVYNGDQLEG
                     VFLIEIVDGLISHFYAMRNPDKLLGIAVPREISRTV"
     gene            1920..>2596
                     /locus_tag="VGP27_15455"
     CDS             1920..>2596
                     /locus_tag="VGP27_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEV7852730.1"
                     /translation="MRTERLGDLGGAPGVLRAVAAAADRHGMAPPAALIGEWFGSGAV
                     IAPSLTVSAVDVGEAFNVPVPRTQADAVGGGWFGYLSYPDAAADGRQGRIPHSAGGWT
                     DCVLRQDSDGHWWYESLSGAALPEWVADALRNTFPPKTFDISWGEADRDAHRAGVINC
                     LAAIAAGEIYQACVCTQFAGRLDGAAIDFFVDAVERASPARAAYLAGEWGSVASLSPE
                     LFLRRVG"
BASE COUNT          409 a          844 c          916 g          427 t
ORIGIN      
        1 ctcgtggaca gcgccagcag gatcaacacc gccgcgcaga ccgtggaatt cgcctcgggt
       61 cgcaggccgg tgcgctacga ctacgtcatc tacgccgtcg gcagcacagg agccgtcccg
      121 gcgtccgttc caggcgcgac cgaattcgct tatccgatag ccgaattgga actggcgcaa
      181 cgactgtgca ccgccatcga cgagctgcac cccgatgcgc cggtcaccgt catcggggcg
      241 ggactgaccg gaatagaggt ggcctccgag ctggccgagc agagccgcaa tgtgacgttg
      301 gtctgcgggg gtcagcttgc cccttcgctg tccgagccgg gccgtcggtc cgtggcaaag
      361 tggatgcaca aacacggcgt gacggtgctg gagtccgacg tcgtggccga ggtccgccgt
      421 ggggcggtgg ttttcgccga cggcgcggtg cgcccgagcg cgatcaccat ctggacggcc
      481 ggtttcggag tgcccgctct ggccgccgac agcgggttgc gcaccgacgc gctgggcagg
      541 ctgctcaccg acgaaaccct gacaagtgtc gacgacgcac gcatcgtggc cgcgggtgac
      601 tgcgccgctc cctcgggccg gccgctgcgg atgagctgcc aagcggcggg cccactcgcc
      661 acgcaggcgg cggacaccgt cttgagtctc atcgcaggcg aggagcctgc tgtcatcgag
      721 ccgagattcg tcgcgcagtg cgtgagcctg ggcaggcacg gggccacact gcaattcacc
      781 cacgaggacg atgcgcctgc gagcttctac ctcggtggtc gcgtggccgc ctccataaag
      841 gaggcagtct gcagaggcac gctgtgggga ctgcgccggg aggcacgcaa gcccggttcg
      901 gcgtttctgt tcaagggctc caagcgtgaa gatgagcccg cggtcgatgc ccaggtggtc
      961 acgaacccgt gaccacctgg ggcgggaacc agattgccgg ctcggccggc ggcgaacacg
     1021 ccgagcggtt cacccacctg cggccgcttc tgttcacgat cgtttacgaa attctcggtt
     1081 cggcaaccga atccgacgac gtgctgcagg acagctatct gcggtgggcc gaggtcgacc
     1141 tcgcgacggt gcgggacacc aagtcgtatc tggcgcagtt ggtgacccgc caggcgctca
     1201 acgcgctacg ggcgggcgcc cgccgtcgcg aggagtacat cggcccgtgg ctacctgagc
     1261 cgctgctgct ggacgaccgt gacgcctcga cggatgtcgt tctcgccgaa tcggtttcga
     1321 tggcgatgct tgtgctgctc gaaactctca ccccggacga gcgggcggtg ttcgtgctgc
     1381 gtgaggtctt cgggttcgac tacgacgaaa tcgccggcgc cgtcgggaag tccgcggtga
     1441 acgtgcgtca ggtggcgcac cgtgcccgcg aacatgtgca ggcgcggcgc aagcgcttcg
     1501 aacccgccga ctcggccaag acggccgaat tgaccgaaca gttcatgacg gcggcgtcga
     1561 cgggtgatgt ggacggcctg atggcgctgc tggcgccgga tgccacgtgg acggccgaca
     1621 gcaacggcaa ggcgacggca gcgcgcaggc ctgtcgtcgg cgcccgcaag gtcgcggcca
     1681 ttctgtccgc cctcttccgt gtcgcggcag agcgcatgcc ggatgtccgg ttcgagacgg
     1741 ccgtctacaa cagctcaccc tcgatcgtgg tgtacaacgg cgaccagctc gaaggcgtct
     1801 tcctcatcga gatcgtcgac ggcttgatca gccatttcta tgcgatgcgc aacccggaca
     1861 agctgttggg catcgcggtc ccgagggaga tcagccgcac ggtctgacaa gcttggtcga
     1921 tgcggaccga gcggctcggc gatctcggcg gcgccccagg ggtgctgcgc gcggtggccg
     1981 ccgctgcgga tcggcacgga atggcgccac ccgccgcgct gatcggcgaa tggttcggct
     2041 cgggcgctgt catcgcgccg tcgttgacgg tgtccgcggt ggatgtcggc gaggccttca
     2101 acgtaccggt gccccgcacg caggccgacg ccgtcggcgg tggctggttc gggtacctgt
     2161 cgtatcccga cgcggctgcc gacggacgcc aagggcgcat tccccactcg gcgggcggct
     2221 ggaccgactg cgtgctgcgc caggacagcg acgggcactg gtggtacgaa agcctctccg
     2281 gcgcagcact tcccgaatgg gttgccgatg cgttacggaa tacgtttccg cccaaaacct
     2341 tcgacatcag ctggggggaa gctgatcgcg acgcccaccg cgcaggagtg atcaactgcc
     2401 tggcggccat cgccgcgggt gagatctatc aggcgtgtgt ctgcacacag ttcgcaggca
     2461 ggctcgacgg cgccgccatc gacttcttcg tcgacgcggt ggaacgcgca tcgcctgcgc
     2521 gtgcggcata tctggcaggc gaatggggta gcgtggcgtc actgtcgccg gagttgttcc
     2581 tgcggcgggt gggcga
//
LOCUS       DASYLX010000652        13965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3654030,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000652 DASYLX010000000
VERSION     DASYLX010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13965
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654030"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1660
                     /locus_tag="VGP27_15460"
     CDS             278..1660
                     /locus_tag="VGP27_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV7852731.1"
                     /translation="MGLSSAVLEFAAEPPGLLPARQQMAVSLGWHIVLACFGVAFPTI
                     IYVMHRRGVVRDDPVALGLAKRWAKVSAVLFAIGAVSGTVLSFEMGLLWPGLMGRFGD
                     VLGLPFAFEGLSFFVEAIFLGIYLYGWGRIPPRRHLAMLIPMGVAGVVGTFCVVSVNA
                     WMNNPTGFRIRAGSVTDVNPWRAMFNDGVWLQFLHMWLAAFMVVGLVVSGVYAAGLLR
                     GRDDAHHRMGFTVPFVFGTVAALAQPIVGHVLGMRIHDTQPAKLAAFELAETTEGPAP
                     FRLGGVLVDGDVHGALEIPRLGSIIARNSLDAPIAGLDTVPKEDWPPVNITHLAFQSM
                     VVIGTLLAAGVVLYWLLRWRGRDLLENRWFLRFSVIAGPLAILAVELGWVATEVGRQP
                     WTVWQVLRTRDAASISTGLWWSYSIVLVTYLGMTIGAWIVLRSMARRWRAGEADLPSP
                     YGPPREESVP"
     gene            1657..2652
                     /locus_tag="VGP27_15465"
     CDS             1657..2652
                     /locus_tag="VGP27_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV7852732.1"
                     /translation="MTLATVVAMAMFVGVVVYALFAGADFGSGFYDLTAGFDERGSEI
                     RTLVDNSIGPVWEANHVWLIYVLVMWWTGFPATFAAATTTLFIPLSLALAGIVLRGAS
                     FAFRKYAETFAQARLFGAIFAASSVITPFFLGAAAGAIASGRVPAQGYGDRVGSWLNP
                     TSIVGGCLAVATSIFLAGVFLTADAYRAGNVSLAERLRTRTLAVGVITGVVVFAGLYP
                     VAHDAPTLSHGLRTAASPLLVLAAVAGVAAVVLLYRRRFSAARAPAVAAVASVVIGWG
                     VGQYPWLLVDQVTIEDGAGADSTLIGLLVVVCLAGVIVIPALIYLLRLTQQWSRA"
     gene            complement(2591..3841)
                     /locus_tag="VGP27_15470"
     CDS             complement(2591..3841)
                     /locus_tag="VGP27_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV7852733.1"
                     /translation="MAFSRTSTATSAGDIYGVIRAAGATTRAELGELTGLSRTAITAR
                     VSTLAAQGLITEREQAPSTGGRPAALLTFNADAGVVLAAAIGRSRTRLAVCNLAGEIL
                     AAGDIDQEIGIGPDELMPDVVKRLDVLMEAHRHARIHGVGLSLPGTVDQHRGCSLDSP
                     IMSGWDGVPLSPYFAELTDAPVILDNDVNVIALAERRGDRQVVDDVLVIKASTGLGAG
                     IIAGGSLQRGAVQAAGEFGHNKTPAADGVPCRCGDTGCLEAIAGGWALVRALTQQGRA
                     VGHIRDVVELANGGDSEARRLIRDSGRHVGEVLAGAVNLLNPALLVVAGDMARAYDIF
                     VAGLRETLYGNATALATRVLQVVPTTYGDRSGVIGSATMVLDNVLSPRLIDESLGASA
                     GKRKFRRGSTVGSAGAGRSAPVSR"
     gene            3986..5200
                     /locus_tag="VGP27_15475"
     CDS             3986..5200
                     /locus_tag="VGP27_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7852734.1"
                     /translation="MTIALERSLTPVSVVAPAPSVRWLAVLALALGGFGIGTTEFVAM
                     GLLPDIASSFTISEPTAGHVISAYALGVVVGAPLIAAFTARMSRKTVLLGLMAVFTIS
                     NLASMFAPSYEMLIVARFVAGLPHGAFFGVAAMVASFLMGPKNRAKAVAHVMTGLTVA
                     TVIGVPMASWLGQALGWRSAFGLVVVVGVMTLTAIAFWLPPLRSMHVTSPLTELGALK
                     RVQVWLAMLVGMVGFGGMFAVYTYISTTMTDVAGMPRALVPLALMVFGLGMVVGNLLG
                     GKMADGSVVRSLYATMSALGVALTVFVLASHNPWTALLVLFAIGAAGSAMGPALQIRL
                     MDVAHDGRTLAACLNHSALNMANAIGALVGGLVIAAGYGYTAPAAAGALLSAAGILVL
                     TGSLALQRRTGA"
     gene            complement(5197..6036)
                     /locus_tag="VGP27_15480"
     CDS             complement(5197..6036)
                     /locus_tag="VGP27_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852735.1"
                     /translation="MTRVSVITGGAGGMGLATAKVVGRDHTLVLADVRQNRLAEAAAA
                     LAGLGIGHKAVNCDVTDREAVDALLDTAAGLGTLASVIHTAGVSPSMGSAEYVMRTNA
                     IGTLNVNESFFAGADEGAAIVNVASMAAHLMPETMLPTEHFPQALHDQDAFMTAMLAA
                     CAIVGEEMQSGIAYAVSKSFVRWYCVAACERFTGKGLRVLSISPGSVETEMGLLEAEA
                     GAGAMVADAAVPRWGKPEEMADLLAFCASDRAGYLTGTDILNDGGVVASMTERARFAA
                     DNS"
     gene            complement(6071..6904)
                     /locus_tag="VGP27_15485"
     CDS             complement(6071..6904)
                     /locus_tag="VGP27_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEV7852736.1"
                     /translation="MPLARTADLVSAAYRAGSGVLAFNVITLESAEAIVSGAEKADRP
                     VILQISENAVKFHRGLLAPIAAAANAVAAGAAVDVSVHLDHVEAEQLLHAGAENGVSS
                     AMFDASKLDYDANVAATKAAADWAHSRGIFLEAELGEVGGKDDAHAPGVRTDPVEAAA
                     FVAATGVDALAVAVGSSHAMSERTASLDFDLIARLRTAVPVPLVLHGSSGVADQDLRK
                     AVSAGITKINVGTLLNVHFTGAVRAYLESDDAVTDPRKYLVPARTGIADAVTTLLRAI
                     G"
     gene            7001..8335
                     /locus_tag="VGP27_15490"
     CDS             7001..8335
                     /locus_tag="VGP27_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV7852737.1"
                     /translation="MAGTLFVGLDVGTTTSKAVVFTQDGDPVASGRAVTPWTVTASGA
                     ELDATALLDAAKAAVTQALADAPPGRVGGLGVASLAESGVLLDRRGESVAPIIAWHDT
                     RDHTELESLRDAIGAQNFSATTGLPLRQQWSLTKHRWLVDNIPSAGSAVRRLNVAEWI
                     VRGLGGEEATEQSLASRTGWLQLADRSWWADTLAWSGADQSLMPTLVAAGTPLGRASA
                     DVGLPRLAGAVLTVAGHDHQAAAVGAGADGPGDELDSCGTAEALVRTISAGLTPDVVA
                     DLADAGITTGWHAVADHWCLLGATQGGLALQRILALLGKDRADLPALDADAIALPMPE
                     IRVVAPAFGTLEVVGITESDSPAHLWRAALETVTEQAADIHDAMTAVSGAHRRLIVTG
                     GWSRSAGLIAVKRRRFGELVQAHVKEAGARGAALLAAKAAAADASTSPAVVS"
     gene            8332..9117
                     /locus_tag="VGP27_15495"
     CDS             8332..9117
                     /locus_tag="VGP27_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV7852738.1"
                     /translation="MTDNLDARTPYADQRKNHILDRLLTSGRVDAVEVAESLGVTGET
                     IRKDLIALERQGLLRRVHGGALPVQALAFEPAVETRTQLASEKTRIAQAALNHLPAEG
                     SVLIDAGSTTAKLVELFPGDLDLTVYTNTVPLAMSLLTRPRLTVFTLGGRLRNVTFAE
                     VGDWAARALAEINVDVAFLGTNGISIERGLTTPDPAEAAVKRLMLGCASKRVLLADHS
                     KFGVVKGTKHGDLGDIDTLISDTGLTDGEYRQLRSAGVEVERA"
     gene            9114..10043
                     /gene="pfkB"
                     /locus_tag="VGP27_15500"
     CDS             9114..10043
                     /gene="pfkB"
                     /locus_tag="VGP27_15500"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV7852739.1"
                     /translation="MIVTLTPNPSVDRTVFVDDVVLGSVNRSRRSRSEPSGKGVNVAL
                     ALHAHDVPVRAVVTAGGSVGAQLQQMLTTAGLDTVVVPIAGEIRSNISLTQPNGLVTK
                     INEAGPQLSPEEADRLLAAVTSHVRGAGWLVCAGSLPAGMPVGWYRKVVEIGHCNGVP
                     VAVDTSGDALAESLPAAPDLVKPNVDELAELTGRVPQTLGDVIDAAQEVRRRGARTVL
                     ASLGGDGAILVDATGAVWGHAPVDKVVSTVGAGDAMLAGYLSCPHGRGESLATALQWG
                     AAAVQNEGTLFSPNVFNAQVTLSATIDLEKRLG"
     gene            10049..11128
                     /locus_tag="VGP27_15505"
     CDS             10049..11128
                     /locus_tag="VGP27_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV7852740.1"
                     /translation="MMQAVVIHGREDYRLERIPVPTPGPGEALVKVRAVGICASDLKC
                     FHGAPMYWGDGPVPPNVDLLVTPGHEFVGEIVAVDDIGRERWGVDTGDRVVAEQIVPC
                     WKCRFCRRGQYWMCAPHSIFGFRHSVNGAMAEYMIYPADAIVHKVSPDVPAAHAAFAE
                     PLSCALHAVERAAITFGDVVVVAGCGPIGLGMIAGAQAKSPAHVIALDHIPAKLELAA
                     ACGADITLNVASDDVDSVVRDLTEGYGADVYLDATGHPSAVPQGLNLLRKLGRYVEYG
                     VFAAPVTVDWTVISDIKELDVLGAHLGPYCWPTAISMIEAKALPLDRICTHQLPLEEF
                     RAGIDLVGRGDESVKVSLLPGAEHS"
     gene            11125..11760
                     /locus_tag="VGP27_15510"
     CDS             11125..11760
                     /locus_tag="VGP27_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV7852741.1"
                     /translation="MTVTVDEFEVADPADAWTRAGFSVDIDSVCRIGGVRIRLVGPDR
                     RVGIVGWSLRGLPSDAALDLDGIPTTVSDAVVATPAEHPNGVTSIDHVVLMSPDLDRT
                     VASLAAAGVEPRRQRDAELGGRPIRQIFFRLGEVIVEVVGSPDSMGEGPPTLWGLTYV
                     VADIDETASFFGDRTAPVKDAVQPGRRITTLRHHEFGMSVRSAMISAPARR"
     gene            11805..12278
                     /gene="rnhA"
                     /locus_tag="VGP27_15515"
     CDS             11805..12278
                     /gene="rnhA"
                     /locus_tag="VGP27_15515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEV7852742.1"
                     /translation="MGMDVVIIHTDGGCRPNPGPGGWGAVLRQREHVREMSGGESGAT
                     SNNRMELTAPIMALEAVTRPVVVHLYTDSTYVRNGITKWVFGWERNGWTTAAKQPVKN
                     VDLWKRLQIACARHQVEWFWIKGHSGVADNELADQLATRGLQEAVEASLPVHLIG"
     gene            complement(12252..>13965)
                     /gene="ligD"
                     /locus_tag="VGP27_15520"
     CDS             complement(12252..>13965)
                     /gene="ligD"
                     /locus_tag="VGP27_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV7852743.1"
                     /translation="AVVLAKRVAQQLEKSMPKLVTSTMTKSLRTGKVFVDWSQNNGAK
                     TTIAPYSLRGREQPTVAAPRTWEEIEDPNLRHLRFDEVLDRVAEYGDLLAGLDEAVPI
                     RDRLTKYRSMRDAAKTPEPVPKSKPKTGGNDRFVIQEHHARRLHYDLRLERDGVLVSW
                     AVPKNLPDTPAVNHLAVHTEDHPLEYLTFEATIPKGEYGAGKMFVWDTGTYETEKFND
                     NPPDGAAKGGEVIVTLHGDKIDGRYALIQTDGKNWLAHRMKEQKRPTAGDLAPMLSTE
                     GSVAKLKKTQWAFEGKWDGYRVLVDADHGNLCVRSRRGRDVTGEFRQFEALAADLADH
                     HVILDGEAVALDDTGVPSFNLMQNRARSTRVEFWAFDILQLDGRSLLRAKYSDRRRIL
                     ETLADGGGVIVPDQLPGDGPEALDYARAQRWEGVVAKRRDSTYQPGRRSASWIKDKIW
                     KTQEVVIGGWREGTGGRSSGIGSLVLGIPGDGGLVYVGRVGTGFTEKDLSNLKKALKP
                     LETDESPFNARLPNPDAKGVTFVQPELVGEVRYSERTGDGRLRQPSWRGLRPDKSPDE
                     VNRE"
BASE COUNT         2106 a         4641 c         4698 g         2520 t
ORIGIN      
        1 tgcgcggacg atcgtcgtgt agacgaaggt gcgttccata ggcgcctgac aggaaccggc
       61 ggatctcaac cgcacctgac accacgaccg gggcacggcc gaactgctga acttttgtct
      121 caccgcttgt cacgggctca cctcatcagg tgacgctctt tcggggctcg agcctcaacc
      181 cccggataac tacacgctac atcgccgccg cgacatcagg tgaaggttga gattgatggt
      241 caccgttgct ccgtcacgaa tcgctaggct ggtccgagtg gggctcagca gtgcggtcct
      301 ggagttcgcg gctgaaccgc cgggactgtt gccggcacgg caacagatgg cggtctcgct
      361 gggttggcac atcgttctcg cctgcttcgg cgtggcgttc ccgaccatca tctacgtgat
      421 gcaccggcgc ggcgtcgtgc gcgacgatcc ggtcgcactc gggctggcga agcggtgggc
      481 caaggtctcg gcggtgctgt tcgctatcgg cgcggtgtcc ggcaccgtcc tcagcttcga
      541 gatgggactg ctgtggccgg ggttgatggg ccgattcggc gacgtgctcg gcctgccgtt
      601 cgccttcgag gggctgtcgt tcttcgtcga ggcgattttt ctcggcatct acctctacgg
      661 atggggccgg attccgccac ggcgtcacct ggcgatgctc atcccgatgg gcgtcgcggg
      721 cgtcgtcggc acattctgcg tggtgtcggt caacgcctgg atgaacaacc cgaccggctt
      781 tcggatccgc gcgggttccg tcaccgatgt gaacccgtgg cgggcgatgt tcaacgacgg
      841 cgtgtggctc cagttcctgc acatgtggct ggcggcgttc atggtggtcg ggctcgtggt
      901 gtccggggta tacgcggccg gcttgctgcg cggccgcgac gacgcacatc accgcatggg
      961 gttcacagtg ccgttcgtct tcggcaccgt cgcagcgctc gcgcaaccga tcgtcggtca
     1021 cgtcctcggc atgcggatcc acgacactca gccggccaaa ctggccgcgt tcgaactcgc
     1081 cgagacgaca gaaggtccgg caccgtttcg gctgggcggc gtcctggtcg acggcgacgt
     1141 gcacggggca ttggagattc cgcggctcgg ctcgatcatc gcccgcaact cgctggacgc
     1201 gcccattgcc ggactggaca ccgtgccgaa ggaggactgg ccgccggtca acatcacgca
     1261 cctggcattc cagtccatgg tcgtgatcgg cacgctgctc gcggcaggtg tggtgctgta
     1321 ctggctgttg cgctggcgtg gtcgggatct gctcgaaaac cgttggttcc tccggttttc
     1381 ggtgatcgcc ggtccgctgg ccatcctcgc ggtggaactc ggctgggtcg ccacggaagt
     1441 ggggcgtcaa ccgtggacgg tgtggcaggt gctgcgcacc cgcgacgccg cgagcatctc
     1501 gacgggactg tggtggagtt acagcatcgt gctcgtgacc taccttggca tgaccatcgg
     1561 agcctggatt gtattgcggt ccatggcccg tcgctggcgt gccggcgagg ccgaccttcc
     1621 gagcccgtac ggtccgccgc gggaggagtc ggtgccatga cgctggcgac cgtcgtggca
     1681 atggccatgt tcgtcggcgt cgtcgtttac gccttgttcg ccggggccga cttcggttcg
     1741 ggcttctacg atctcacggc cggattcgac gagcggggta gtgagattcg caccctcgtc
     1801 gacaacagca tcggaccggt gtgggaagca aaccatgtgt ggcttatcta cgtcctcgtc
     1861 atgtggtgga ccggattccc cgcgactttt gccgcagcca ccacgacgct gttcatcccg
     1921 ctcagcctgg cgctcgcggg aattgttctg cgcggagcca gctttgcgtt ccgtaagtac
     1981 gccgagacat tcgctcaggc acgtctgttc ggcgcgatct tcgcggcctc gtcggtgatc
     2041 actccgttct ttctcggcgc tgccgcgggc gccatcgcgt ccgggcgggt gcctgcgcag
     2101 ggttacggcg atcgtgtcgg gtcctggctc aatccgacgt cgatcgtggg cggctgcctg
     2161 gccgttgcga cgtccatctt cctcgcgggg gtgtttctca ccgcagacgc ctaccgcgcc
     2221 gggaacgtca gcctcgccga gcgcctgcgc acccgtaccc tcgcggtcgg cgttatcacc
     2281 ggcgtcgtcg tattcgcggg gttgtacccg gtcgctcatg atgcgccgac gctgagccac
     2341 ggcctgcgca ctgccgcgtc gccgttgttg gtgctggccg cggtcgcggg cgttgcggcc
     2401 gtcgtgctgc tgtatcgccg tcgtttctcg gccgcgcggg cgcctgcggt ggcggccgtc
     2461 gcttcggtcg tcatcggttg gggcgtcggc caatatccgt ggctgctcgt ggatcaggtg
     2521 accatcgagg acggcgccgg cgccgattcc accctgatcg gccttctggt cgtggtctgc
     2581 cttgccggtg tcatcgtgat accggcgctg atctacctgc tccggctgac ccaacagtgg
     2641 agccgcgcct gaacttccgc ttccccgcac tcgcaccgag tgactcgtcg atcaggcgtg
     2701 ggctcagcac gttgtcgagg accatggttg cgctgccgat gacgccagac cggtcaccgt
     2761 atgtagttgg cactacttgg agcacgcggg ttgcgagcgc agtcgcgttg ccgtacaacg
     2821 tttcccgcag acccgccacg aagatgtcgt atgccctcgc catatcgcct gcgacgacca
     2881 gcagcgcggg attgagcaga ttgaccgcgc ctgcgagaac ctcgccgacg tgacgaccgc
     2941 tgtcgcggat gagcctgcgc gcttcggagt caccgccgtt ggccagttcc acgacgtcgc
     3001 ggatgtggcc gaccgcgcgg ccttgttgcg tcagcgcccg caccagcgcc caaccgccag
     3061 cgatcgcttc cagacagccg gtgtcgccgc atcggcacgg cacgccgtcg gcggcgggcg
     3121 tcttgttgtg accgaactcg ccggcagctt gtaccgcgcc acgctgtagc gaacccccgg
     3181 cgatgatgcc tgctccgaga cccgtcgagg ccttgatgac gagcacatcg tcgaccacct
     3241 ggcggtcgcc ccgccgctca gccagggcga tgacattgac gtcgttgtcg aggatgaccg
     3301 gcgcatcggt cagttcggcg aagtagggcg acagcggtac cccgtcccaa ccgctcatga
     3361 tcggcgagtc gaggctacag ccgcggtgct ggtcaaccgt cccgggcagg gacagcccga
     3421 cgccgtggat gcgggcatgg cggtgcgcct ccatcaggac atcgagcctc ttgaccacgt
     3481 ccggcatcag ctcatcggga ccgatgccga tctcctggtc gatgtcgccc gcggccagaa
     3541 tctcgccggc gaggttgcac accgccagcc gggtccggct acgcccgatc gcggcggcca
     3601 gcacgacacc ggcgtcggcg ttgaacgtca gaagtgcggc ggggcggcca cccgtggacg
     3661 gcgcttgttc acgttcggtg atcaggccct gggccgccag ggtcgacacg cgggcggtta
     3721 tggcggtgcg ggacaacccg gtcagttcac cgagctccgc gcgtgtcgta gcacccgcgg
     3781 cgcggatcac gccgtaaata tcgccggccg aggttgccgt cgaagtacgg gaaaaggcca
     3841 tgtcatcgat tccgttaggg ccgatcgcgg gttgtctcca atcgtaacct gcttcacttt
     3901 tgccgtcaaa aagccgaagt cggtttgcag agggccgaaa gtctcgttac cgttgcctcc
     3961 gtccgaccag aaaggtccaa cgtttgtgac catcgcgctt gaacgttcct tgacgcccgt
     4021 ctccgtcgtc gcgcccgctc cgtcggtgcg gtggcttgcg gtgctggcgc ttgccttagg
     4081 tgggttcggc atcggaacca ccgagttcgt cgcaatggga ctgctgccgg atatcgcgtc
     4141 cagtttcacc atctccgaac cgactgccgg acatgtgatt tcggcgtacg cactcggcgt
     4201 ggtcgtcggg gcgccgctga ttgccgcttt caccgcgcgg atgtcccgaa agacggtgct
     4261 gctgggactg atggcggtat tcaccatcag caacctggcc agcatgtttg cgccgtccta
     4321 cgaaatgctg atcgtcgccc gcttcgtcgc cggtctgcct cacggggcgt tcttcggcgt
     4381 cgccgccatg gtggcgtcct tcctgatggg accgaagaac cgggccaaag ccgtcgcgca
     4441 cgtgatgacg gggctcaccg tcgcgaccgt tatcggtgtg ccgatggcct cgtggctcgg
     4501 ccaggccctt ggctggcgca gcgccttcgg tttggtcgtc gtcgttggag tgatgaccct
     4561 gaccgcgatc gcattctggc tcccgccgct gcgatcgatg cacgtgacca gtccgctcac
     4621 ggagctcggc gcgctcaaac gcgtgcaggt gtggttggcg atgctcgtcg gcatggtcgg
     4681 attcggtggc atgttcgccg tgtacaccta catcagcacc acgatgaccg atgtcgcggg
     4741 catgccccgc gcgctggttc cgttggcgct catggtgttc ggcctcggca tggtggtggg
     4801 gaatctgctc ggcggcaaga tggccgacgg ttcggtggtt cgaagcctgt acgcgacgat
     4861 gagcgccctt ggcgtcgcat tgacggtgtt cgtgttggcc tcccacaacc cgtggacggc
     4921 gctgctggtc ttgttcgcga tcggcgccgc aggctcggcg atggggccgg cactgcagat
     4981 ccggctgatg gacgtcgcgc acgacggcag aacgttggcg gcctgcctca atcactcggc
     5041 tctgaacatg gccaacgcga tcggcgctct ggtcggcggg cttgtcatcg cggccgggta
     5101 cggctacacc gcgcccgcgg ccgcgggcgc gctgctttct gccgcgggga tactggttct
     5161 caccggctca ttggcgttgc agcgaaggac cggcgcctag ctgttgtcgg cggcgaacct
     5221 ggcccgctcg gtcatcgacg ccaccacccc gccgtcgttg aggatgtcgg tgccggtgag
     5281 atagccggcc ctgtcgctgg cgcagaacgc cagcaggtcg gccatctcct ccggcttgcc
     5341 ccagcgcggc actgcagcgt cggcgaccat ggcgcctgca cccgcctcgg cttccagcag
     5401 gcccatctcg gtctcgacgg atcccggtga gatcgagagg acacgcaggc ctttgccggt
     5461 gaaccgttcg cacgcagcga cgcagtacca ccgcacgaag ctcttgctga cggcgtaggc
     5521 gatgcccgac tgcatctcct caccgacgat cgcgcacgcc gcaagcatgg ccgtcatgaa
     5581 cgcgtcctga tcgtgcagcg cctgcgggaa atgctctgtc ggaagcattg tttcgggcat
     5641 caggtgcgcg gccatcgacg cgacgttgac gatcgcggcg ccctcgtccg cgccggcgaa
     5701 gaagctctcg ttgacgttga gcgtgccgat cgcgttggtc cgcatgacgt attcggccga
     5761 gcccatgctc gggctcaccc cagcggtgtg aatcaccgac gcgagcgtgc cgagaccggc
     5821 cgcggtatcg agcagagcat cgaccgcctc gcggtcggtg acatcgcagt tgacggcctt
     5881 gtggccgatt ccgaggccgg cgagcgcggc ggccgcttcg gcgaggcggt tctggcggac
     5941 gtctgcgagg acgagggtgt ggtcgcggcc cacgaccttc gccgtcgcca gtcccatgcc
     6001 gcccgcacca ccggtgatca ccgagactct ggtcataagg cgatgctatg cgactgccta
     6061 gcgctcgcgg tcagccgatc gcgcgcagca gcgtggtcac cgcgtctgcg atgcccgttc
     6121 gcgcaggcac caggtacttg cgcggatcgg tgacggcgtc atcggactca aggtaagcgc
     6181 ggaccgcccc ggtgaagtgg acgttgagca gggtgccgac gttgatcttc gtgatgccgg
     6241 cgctgacggc cttgcggagg tcttggtcgg caaccccgga ggagccgtgc agcacgagcg
     6301 gcacgggcac cgccgtccgc agccgtgcga tgagatcgaa atccaatgac gcggttcgct
     6361 cggacatggc atgggagctg ccgacggcga ccgccagggc gtcgacgccc gtcgcggcga
     6421 cgaacgcggc ggcttccacc gggtcggtgc ggaccccggg cgcgtgcgcg tcgtcctttc
     6481 caccgacctc gccgagctct gcttcgagga aaatcccgcg gctgtgtgcc cagtccgctg
     6541 ccgccttggt ggccgctacg ttggcgtcgt aatcgagctt ggaggcgtcg aacatcgctg
     6601 agctgactcc gttttcggcg cccgcatgca ggagctgttc ggcctcgacg tgatcgaggt
     6661 gaaccgatac gtcgacggcg gccccggcgg cgaccgcatt cgccgccgcc gcgatcggcg
     6721 ccagcaggcc gcggtggaac ttcactgcgt tctcgctgat ctgaaggatc accggccggt
     6781 cggccttctc ggcgcctgag acgatcgcct cggcggactc gagcgtgatg acgttgaacg
     6841 ccagaactcc cgagccggcg cggtacgcgg ctgaaaccag gtcggcggtt cgggcgagtg
     6901 gcatccgagg tccttacgag tcgcttgaca ttgctccaac aaaacgcaac tatatccatc
     6961 agattccaat tcgcacaacc ctcgaccgtc aaggagcgct atggccggca cgctgttcgt
     7021 cggcctcgac gtcgggacaa ccaccagcaa ggccgtggtc ttcacccagg acggcgaccc
     7081 cgtcgcgtcc gggcgcgccg tcacgccgtg gacggtcacg gcttctggtg ccgagctcga
     7141 tgcgacggct ctgctcgacg ccgccaaagc cgcggtcacc caggcgttgg ccgacgctcc
     7201 gcccggacgt gtcggcggcc tcggcgttgc aagcctcgcc gagtcgggcg tgctgctcga
     7261 ccgacgtggc gagtcggtgg ccccgatcat cgcctggcat gacacccgcg accacacaga
     7321 gttggaaagc cttcgcgacg ccattggcgc acaaaacttc tcggcgacga ccggattgcc
     7381 gctgcgtcag cagtggtcgc tgacaaagca ccggtggttg gtcgacaaca tcccctcggc
     7441 gggtagcgca gttcgccgcc tgaacgtcgc ggagtggatc gtgcgcggcc tcggcggtga
     7501 ggaagccacc gaacaatcgc tcgcatctcg cactggatgg ctgcaactgg ctgatcggtc
     7561 gtggtgggcg gacacgctgg cgtggtcggg ggctgaccaa tctctgatgc cgacgttggt
     7621 ggcggcgggc actccgctcg gacgcgcgag cgccgacgtc gggttgccga ggttggccgg
     7681 tgctgtgctg accgtcgcgg gccacgatca tcaagccgcc gcggtcgggg cgggggcaga
     7741 cggacccggc gacgaactcg attcctgcgg taccgcagaa gcactcgtgc ggaccatatc
     7801 cgctggcctt acccccgatg tcgtcgccga cctcgccgat gcgggcatca ccaccggctg
     7861 gcacgccgtc gccgaccact ggtgtctgct cggcgccacc cagggtgggc tcgcgcttca
     7921 acgcatcctc gcgctgctcg gcaaggacag ggctgacctg cccgcactgg acgccgacgc
     7981 catcgccttg ccgatgcctg agatacgggt cgtggcaccg gcattcggca ccctcgaggt
     8041 cgtcggcatc accgaatccg acagcccggc ccatctgtgg cgcgccgcct tggagacagt
     8101 gaccgagcag gccgccgata tccacgacgc catgaccgcc gtcagcggag cgcaccggag
     8161 gttgatcgtc accggcggct ggtcgcgcag cgccgggctc atcgcggtca agcgccgtcg
     8221 cttcggtgaa ctggtgcagg ctcacgtcaa ggaggccggt gcccgcggtg cggcgctgct
     8281 ggcggccaag gcagccgctg ccgatgcgtc gacctctccc gcggtggtgt cgtgaccgac
     8341 aaccttgatg cccgcacccc gtacgccgac cagcgcaaga accacatcct cgatcggctc
     8401 ctgacgagcg gccgcgtcga cgccgtggaa gtcgccgagt cgcttggagt gaccggggag
     8461 acgatccgca aggatctcat cgcactcgag cggcaaggcc tgctgcgacg tgtgcacggc
     8521 ggcgcgctgc cggtccaggc actggcgttc gagcctgcgg tggaaacgcg aacacagttg
     8581 gcgtcggaga agactcgcat cgcccaggcg gcgctgaacc atctgcccgc ggagggctcg
     8641 gtgctcatcg acgcgggttc cacgacggcc aaactggtgg aacttttccc cggcgacctc
     8701 gacctgacgg tctacaccaa caccgtgccg cttgcgatgt cactgctgac ccggccccgg
     8761 ctcaccgtct tcacgctcgg cgggcggctg cgcaatgtta ccttcgccga ggtcggcgac
     8821 tgggctgcgc gggcactggc agagatcaac gtcgacgtcg cgttcctggg cacgaacggc
     8881 atctcgatcg aacgcgggct gacgaccccg gaccccgccg aggcggccgt caaacgactg
     8941 atgctcggct gcgcttcgaa gcgcgtgctg ctggccgacc acagcaagtt cggcgtcgtc
     9001 aagggcacca aacacgggga cctcggcgac atcgacacgc tgatcagcga taccggcctg
     9061 accgatggcg agtacaggca attgcgcagc gccggagtcg aagtggagcg agcatgatcg
     9121 tcaccctgac gcccaatccg agcgtggacc gcaccgtgtt cgtcgacgat gtcgtgcttg
     9181 gttcggtcaa ccgcagtcga cgcagcagaa gcgagccaag cggaaaaggc gtcaatgtcg
     9241 ctctcgcgct gcacgcgcac gacgtgccgg tgcgcgctgt ggtcaccgca ggcggttccg
     9301 tcggcgcgca gctgcagcag atgctcacca ccgcggggtt ggacaccgtc gtcgttccca
     9361 tcgcagggga gatccgcagc aacatcagcc tgacccagcc gaacggtttg gtcaccaaga
     9421 tcaacgaggc aggcccgcaa ctgagcccag aagaagccga ccgactgctg gccgccgtca
     9481 cctcgcacgt ccgcggcgca ggctggttgg tgtgcgcggg cagcctgcct gcgggcatgc
     9541 cggtcggctg gtaccgcaaa gtcgtcgaaa tcggacactg caatggtgtg cccgtcgccg
     9601 tcgatacctc aggagatgcg ctcgccgaaa gccttcctgc ggcaccggat ctggtgaagc
     9661 caaatgtgga cgagctcgcc gagctgacag gtcgggtgcc gcagaccctc ggcgacgtca
     9721 tcgacgccgc gcaggaggta cgccgccgcg gcgcccgtac ggtgcttgcc agccttggcg
     9781 gcgacggcgc gatcctcgtc gacgccacgg gcgccgtgtg gggccacgcg cctgtcgaca
     9841 aggtggtcag caccgttggc gcgggagacg cgatgctagc cggctatctc agctgcccgc
     9901 acggccgcgg cgagtcgctg gcgaccgccc tgcagtgggg tgctgccgcc gttcagaacg
     9961 agggaacctt gttctccccc aacgtgttca acgcacaggt gacgttgagt gccaccatcg
    10021 atctcgaaaa gaggctgggt tgagctcgat gatgcaggcg gtcgtgattc acggtcgcga
    10081 ggactaccgc ctcgagcgga tacccgttcc gacccccggc cccggtgaag cgttggtgaa
    10141 ggtacgggcc gtagggattt gcgcaagcga cctcaagtgc tttcacggcg cacccatgta
    10201 ctggggcgac gggccggtgc cgccgaacgt cgacctgctg gtgacgcccg gccacgagtt
    10261 cgtcggcgaa atcgtggccg tcgacgatat cggtcgggag cggtggggcg tcgacaccgg
    10321 cgaccgcgtc gtcgccgagc agatcgtccc gtgctggaag tgccggttct gccgtcgcgg
    10381 ccagtactgg atgtgcgccc cgcacagcat cttcgggttt cgccacagcg tcaatggggc
    10441 gatggcggag tacatgatct accccgccga cgcgatcgtg cacaaggtgt cgcccgacgt
    10501 tcccgcggcg catgcggcgt tcgccgagcc gttgtcgtgt gcgctgcacg ccgtcgagcg
    10561 tgcggcgatc accttcggtg acgtcgtggt ggtcgccggc tgcggtccga tcggactggg
    10621 catgatcgcg ggcgcgcagg cgaagagccc agcccatgtg atcgccctcg atcacattcc
    10681 tgccaagctc gaactcgccg cggcgtgcgg ggccgacatc acgttgaatg ttgcgagcga
    10741 tgacgtggat tcggtggtgc gcgatctgac cgagggctac ggcgccgatg tgtacctcga
    10801 tgcgaccggc catccatccg cggtcccgca ggggctgaac ctgctccgca agctgggccg
    10861 ctacgtcgag tacggagtgt tcgcggcacc ggtcaccgtc gactggacgg tcatcagcga
    10921 tatcaaggaa ctcgacgttc tcggcgcgca cctcgggccg tactgctggc cgacggcgat
    10981 atcgatgatc gaggccaaag cgctaccgct ggatcgcatt tgcacacatc agcttccgct
    11041 ggaggagttt cgcgctggca tcgacctcgt cggccgcggc gacgaatccg tcaaggtgag
    11101 cctgctgccc ggcgcggaac actcgtgacc gtcaccgtcg acgaattcga ggtcgccgat
    11161 ccggcggacg cgtggacgcg ggccggattc agcgtcgaca tcgattccgt ctgtcggatc
    11221 ggcggtgttc gcatccggct ggtcggccct gaccgccgag ttggaatcgt cgggtggtca
    11281 ctgcgcggcc ttccgtcgga cgccgcgctc gacctcgacg gcattccgac gacggtgtcg
    11341 gacgcggtcg ttgcgacgcc ggcagagcac ccgaacggtg tcacctcgat cgaccacgtc
    11401 gtgctgatgt caccggacct cgaccggacg gtcgcgtcgc tggcggctgc cggtgtcgag
    11461 ccccgacggc aacgcgacgc cgaactcggc gggcgaccga tccgccagat cttcttccga
    11521 ctcggggagg tgatcgtcga ggtcgtcgga tcaccggatt cgatgggcga gggtccaccc
    11581 acgctctggg gtctgacgta tgtcgtggcg gatatcgacg agaccgcatc gttcttcggc
    11641 gaccgcaccg caccggtgaa ggacgcggtg cagccggggc gacggatcac cactctgcgg
    11701 caccacgagt tcgggatgtc ggtccggtcg gcgatgatct cggcacccgc gcgtcgctga
    11761 agccgactgc gcgacccagt gcctatcctc ggcgcatgac cgacttgggc atggacgtgg
    11821 tgatcatcca caccgacggc ggatgcagac ccaacccggg gcccggcggc tggggtgcgg
    11881 tgctgcgcca gcgtgaacat gtccgggaga tgtccggcgg cgagtcgggc gcgacaagca
    11941 acaacaggat ggagctgacc gcgccgatca tggcgctgga ggcagtcacc agacccgttg
    12001 tggtgcacct ctataccgac agcacctatg tccgcaacgg gatcaccaag tgggtgttcg
    12061 gctgggaacg caacggatgg acgaccgctg ccaagcaacc ggtgaagaac gtcgatctgt
    12121 ggaagcggct tcaaatcgcc tgcgcccggc accaggtcga gtggttctgg atcaaggggc
    12181 actcgggtgt cgccgacaac gagttggccg accagttggc caccaggggt ttgcaggaag
    12241 cggtcgaggc gtcactcccg gttcacctca tcgggtgact tgtccggtcg cagaccacgc
    12301 caactcggct ggcgcagtcg gccatcgccc gtccgttcgc tgtagcggac ctcgccgacg
    12361 agttctggct ggacaaacgt gacacccttg gcgtccggat tgggaagcct tgcgttgaag
    12421 ggtgattcgt cggtttccag cggcttcaac gccttcttca gattgctgag gtccttctcg
    12481 gtgaagccag tgccgacccg accgacgtag acaaggccgc cgtcgcccgg gatgcccagc
    12541 accagcgaac cgatgccgct gctgcgcccg ccggtacctt cgcgccagcc gccgatgacg
    12601 acttcctggg tcttccagat cttgtccttg atccacgacg ccgagcgcct acccggctgg
    12661 taggtcgaat cgcgcctctt ggcgaccaca ccttcccagc gttgcgcgcg ggcgtagtcc
    12721 agcgcctcgg gcccgtcgcc gggcagttga tccgggacga tcacccctcc cccgtcggcc
    12781 agcgtctcca agatccgtct gcggtcggag tacttggccc gcaacagcga gcggccatcc
    12841 agttgcagaa tgtcgaacgc ccagaactcg acgcgggtgg agcgggcacg gttctgcatc
    12901 aggttgaagc tgggcacgcc cgtgtcgtcg agggccaccg cctcaccgtc gaggatcacg
    12961 tggtggtcgg cgagatcggc cgcgagcgcc tcgaattggc ggaactcgcc ggtgacgtcg
    13021 cgcccgcggc gcgatcgcac acagaggttg ccgtgatcgg cgtccaccag aacccgatag
    13081 ccgtcccact tgccctcgaa ggcccattgc gtcttcttga gcttggccac cgacccttct
    13141 gtggacagca tcggcgcgag atcgcctgcc gtgggccgtt tctgctcctt catccgatgg
    13201 gccaaccagt tcttcccatc cgtctggatg agcgcatacc ggccgtcgat cttgtcgccg
    13261 tggagggtga cgatcacctc cccgcccttt gcggctccgt cgggcggatt gtcgttgaac
    13321 ttctcggtct cgtaggtgcc ggtgtcccag acgaacatct tcccggcgcc gtactccccc
    13381 ttcgggatcg tcgcctcgaa tgtcaggtac tccagcgggt ggtcctcggt gtgcacggcc
    13441 agatggttga ccgcgggcgt gtcgggcagg ttcttcggca ccgcccacga caccaggacg
    13501 ccgtcgcgct cgaggcggag gtcgtagtgc aaccggcggg catggtgttc ctgtatgacg
    13561 aagcggtcgt ttccgcctgt cttcggcttc gacttcggca ccggctcagg ggttttcgcg
    13621 gcatcccgca tgctgcggta cttcgtcagc ctgtcgcgga tgggtaccgc ttcgtccagc
    13681 cccgccagca ggtcaccgta ttccgccaca cgatcgagca cctcgtcgaa gcgcaggtgc
    13741 cgcaggttgg ggtcctcgat ctcctcccag gtacgcggag cggccaccgt cggctgctcg
    13801 cgcccgcgta gcgagtacgg cgcgatcgtc gtcttcgccc cgttgttctg gctccagtcg
    13861 acgaacacct tcccggtccg caggctcttg gtcatcgtcg aggtgacgag cttcggcatc
    13921 gacttctcta gttgctgggc aacgcgtttg gccaagacga cggcg
//
LOCUS       DASYLX010000653         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1680125,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000653 DASYLX010000000
VERSION     DASYLX010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680125"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGP27_15525"
     CDS             complement(<1..308)
                     /locus_tag="VGP27_15525"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEV7852744.1"
                     /translation="MLPCIGLAALGLTVSTSSLQTVVANGDTRSISLSHTHRDDQITV
                     TFKRNGRYDDDGLKKLNYFLRDWRNDEQTRMDPQLFDILWEITREVNTKEPIRIVSSY
                     R"
     gene            complement(594..>2233)
                     /locus_tag="VGP27_15530"
     CDS             complement(594..>2233)
                     /locus_tag="VGP27_15530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7852745.1"
                     /translation="RKADRTAVEAFYSSRDYAPLFYGTNGATAIGKQAINHLRNADAD
                     GMDPTDYPVPSMTADMAPAALAEAEIKLTDAVLDYARHATLGRVHYSRVSGDIFYEQV
                     APQPLDVLAKVAATNNAEEALETYQPQHAAYKALRKKLAEMRGKGETPEAKIPHGPVL
                     EIATDKKSKQTALMTDERVPALRAKLGIDPVKGDTFYDKPLADAVAHYQKEKGLPSNG
                     RLTQATVDAMNGKRSERTDQLIIANMERWRWVPRDLGKAHVVLNIPDYTLQVYNNGAS
                     IWKTRVVVGKPGNHATPLLSETMKYITVNPTWNVPPSIVYNEYLPALQQDPTVLKRMG
                     LNLSQNRDGSVHISQPPGEANALGRVRFNFPNKFLVYQHDTPDKHLFAHPKRAYSHGC
                     MRVQDPAKYAEVITGLALPGGKSYTADQIKGMFGHSEIDLRFTHQIPVHITYQSAFVD
                     ENGTLQIRDDIYGRDAQTLAALKTDRRQADVPVARSQPNYSRPSVRLPSGVGGGNDWS
                     ANSGGGQSFFDRLFGNPVRQEPAPQRNRRVQRQVTSR"
BASE COUNT          338 a          677 c          774 g          444 t
ORIGIN      
        1 cggtaggacg agacgatgcg gatcggttcc ttggtgttca cctcgcgggt gatctcccac
       61 aggatgtcga acagctgcgg gtccatccgc gtctgctcgt cgttgcgcca gtcgcgcagg
      121 aagtagttga gcttcttcag cccgtcgtcg tcgtagcggc cgttgcgttt gaaggtgacg
      181 gtgatctggt cgtcgcggtg cgtgtgcgag agcgagatcg aacgggtgtc gccattggcg
      241 accacggtct gcagtgaact ggtgctgacg gtcagcccaa gcgcggcgag cccgatgcac
      301 gggagcacgc gaagaggaat ccagatcgat aaagcctggc gcgcaaagcg tctcggcacg
      361 tcaaatcccg tccagaacgt cacttcgtcg tgtgaccgcg gcgtcccccg ccgcgtgcgt
      421 gtgattaagg cgcagtctta tcgggatggg ttaacgcgag attgagcgtc gcccgtcccc
      481 cctcagggtt aattcctcta agggggcagt gtggcaaaaa atagtcctga cggaacaggg
      541 gctttcccgg ccggtcggca gggttaacga gacgtaaccg gttggctctc cggttagcgg
      601 ctcgtcacct gacgctgcac gcgacgattg cgctgcggtg ccggctcctg gcggaccggg
      661 ttgccgaaca gccggtcgaa gaaggactgc ccgccgccgc tgttggcgct ccagtcgttg
      721 ccgccgccaa cgccgctcgg caggcggact gacggacggc tgtagttcgg ctgcgagcga
      781 gccaccggga cgtcggcctg ccggcggtcg gtcttgagcg ccgccagcgt ctgcgcatcg
      841 cggccgtaga tgtcatcgcg gatctgcagc gtgccgtttt catcgacgaa ggcggattgg
      901 taggtgatgt gcaccgggat ctggtgggtg aaccgcagat cgatctcgga gtggccaaac
      961 atccccttga tctgatcggc cgtgtagctc ttgccgcccg ggagcgcgag gccggtgatc
     1021 acctcggcgt acttcgccgg atcctgcacc cgcatgcagc cgtggctgta ggcgcgcttg
     1081 ggatgcgcga acaggtgttt gtccggggta tcgtgctgat agaccaggaa cttgttcggg
     1141 aagttgaagc gcacgcggcc gagcgcgttg gcctcacccg gcggctgcga gatgtgcacc
     1201 gagccgtcac gattctggct caggttcagc cccatgcgct tgagcacggt cggatcctgc
     1261 tgcagggcgg ggagatactc gttgtagacg atcgacggcg gcacgttcca agtcgggttg
     1321 acggtgatgt acttcatcgt ctcggagagg agcggcgtcg cgtgattgcc cggcttgccg
     1381 acgacgacgc gggttttcca gatcgacgcg ccgttattgt agacctgcag cgtatagtcc
     1441 gggatgttca gcaccacgtg cgccttgcca aggtcgcgcg gcacccagcg ccagcgctcc
     1501 atgttggcga tgatgagctg atcggtgcgc tccgagcgct tgccgttcat cgcatcgacc
     1561 gtcgcctgcg tgagccggcc gttcgacggg aggccctttt ccttctggta gtgcgcgacc
     1621 gcgtcggcga gcggcttgtc gtagaaggtg tcgcccttca ccggatcgat gccgagcttt
     1681 gcgcgcagcg ccggcacgcg ctcgtcggtc atcagcgcgg tctgcttgct cttcttgtcg
     1741 gtcgcgatct caagcaccgg gccgtgcggg atcttggcct cgggcgtctc gcccttgccg
     1801 cgcatttcgg cgagcttctt gcgcagcgcc ttataggcgg cgtgctgcgg ctgataggtc
     1861 tccagcgcct cctcggcgtt gttggtggcc gccaccttgg ccagcacgtc gagcggctgc
     1921 ggcgcaacct gctcgtagaa aatgtcgccg gacacgcgcg aataatgcac gcggccgagc
     1981 gtggcgtggc gcgcatagtc gagcaccgcg tcggtcagct tgatttcggc ctcggcgaga
     2041 gcggccggag ccatgtccgc cgtcatcgac gggaccggat agtcggtcgg atccatgccg
     2101 tcggcgtcgg cgttgcgcag atggttgatc gcctgcttgc cgatcgcggt cgcgccattg
     2161 gtgccgtaga acagcggcgc gtaatcgcgc gacgaataga acgcctcgac ggcggtgcga
     2221 tccgctttgc gat
//
LOCUS       DASYLX010000654         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2556597,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000654 DASYLX010000000
VERSION     DASYLX010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556597"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1746
                     /locus_tag="VGP27_15535"
     CDS             52..1746
                     /locus_tag="VGP27_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7852746.1"
                     /translation="MTAALRNWGGIGPGSSDAELAVAATDGDRRAFAEIYDRYADRLN
                     DFCIGMLRDRDSAADCVQDAFCIVSTSLGGLREPDKLRPWLYSIVRNEAHKRLRDLKR
                     ERPSDEMPDVVSHDAGPDTMAQRMELANLISEAAGGLSDRDQAVLELAYRQGLDGPEL
                     AMALGVTQTNANTMVGRLRETIERSLGALLVARRNQSDPGRCQGLSAVLQGWDGKFNI
                     LMRKRIARHIESCAACEELRARSVNPAALLGAPLFIPAPAWLKNNVMRDFELTSASHR
                     MENSSHNAATRMSPRADQSDFHAASTAARPAIQPPPPPYAPQHEEPTAFVETTSDEDK
                     RRVVPFMALLIGAPLVILGVTLAWVYLPSTALSPSGIVAPAPLPTPTPSVPAPTPAAV
                     LPTPQAPLATPAPKPVATPRPGAALPTPQAKIPTPQPQAPLPTPQAPAQAPAPVQAPA
                     PAPIPQLPVGSLPFPMPRMPVPQNPAQLPAQLPAQAPAQIPPQQPPAQAPAKVPAQQP
                     PAQVPDPVGPAPKQVPDPVGPAPAQVPDPVGPAPKQVPAPEPAQKACDPNVDICVK"
BASE COUNT          305 a          652 c          539 g          259 t
ORIGIN      
        1 cagcaactac gctgacacca aggccacttg atcaacctgg agtaatacgc catgaccgcc
       61 gcactgagaa actggggtgg catcgggccc ggcagcagtg acgccgaact cgccgtcgcg
      121 gcgaccgacg gtgaccgtcg ggcgttcgcc gagatctatg accgctacgc cgaccggctg
      181 aacgacttct gcatcggaat gctccgcgat cgcgactcgg ccgccgactg tgtccaggac
      241 gccttctgca tagtgtccac cagcctcggt ggtctgcgcg agcctgacaa gctgcgcccg
      301 tggctctact ccatcgtccg caacgaagcc cataagagac tgcgggacct caagcgcgaa
      361 cgaccatctg acgagatgcc cgacgttgtg tcgcacgacg caggcccgga cacgatggct
      421 cagcggatgg agttggccaa tctcatctcc gaagccgccg gcggactgtc cgatcgcgac
      481 caggccgtgc tggagttggc ttaccgccag gggctggacg gacccgaact cgcgatggcc
      541 ctgggggtca cccagacgaa cgccaacacc atggtcggcc ggctgcgcga aaccatcgag
      601 cgctcgctcg gcgccctgct ggtggcgcgc cgcaatcaat cggatccggg ccgctgccaa
      661 gggctgtcgg ctgtattgca gggctgggac gggaaattca atattctgat gcgcaagcgc
      721 attgcgcgcc atatcgagtc gtgcgccgcg tgcgaagagt tgcgcgccag atcggtgaac
      781 ccggccgccc tgctcggcgc gccgttgttc attcccgccc cggcgtggct gaagaacaac
      841 gtcatgcgtg acttcgagct gacgtcggcg tctcatcgca tggagaactc atcgcacaac
      901 gcggcgacgc ggatgagtcc cagagcagat cagtcagact ttcacgcggc gtcgaccgct
      961 gcccggccgg cgatccagcc accaccgccg ccgtatgcac cacaacacga ggagccgaca
     1021 gcattcgtcg agaccacatc cgacgaggac aagcgtcgtg tggtgccgtt catggcgttg
     1081 ttgatcggtg ccccgcttgt gatcctcggg gtgacgctgg cctgggtgta tctcccgagc
     1141 accgcgcttt cgccgagtgg catcgtggcg ccggcgccgc taccgacgcc gaccccgtcc
     1201 gttcccgcgc cgacccccgc cgcggttctt cccacgccgc aggcgcccct cgcgaccccg
     1261 gctccgaagc cggtggcgac tccgcgtcct ggcgctgcgc tgccgacgcc acaagccaag
     1321 attccgaccc cgcaacccca ggcgccgctg ccgacgcctc aggcacctgc ccaagcacct
     1381 gcccccgtgc aggcgcccgc gccggcaccg atcccgcaat tgccggtggg atcacttccg
     1441 ttcccgatgc ccaggatgcc cgtgccgcag aacccggctc agcttccggc gcagttaccc
     1501 gcgcaagcgc ccgctcagat cccgccgcaa cagccgccgg cacaagcacc cgcgaaggtc
     1561 ccggcccagc agccgcccgc acaggtgccc gatccggtcg gaccggcacc caagcaggtg
     1621 cccgatcccg tcggtcccgc acccgcacag gtgcccgatc ccgtcggtcc ggcacccaag
     1681 caggtccccg cgcccgaacc tgcacagaag gcgtgcgatc cgaacgtgga catctgcgtc
     1741 aagtagcgcc gtaga
//
LOCUS       DASYLX010000655         5681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_472808,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000655 DASYLX010000000
VERSION     DASYLX010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472808"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGP27_15540"
     CDS             <1..188
                     /locus_tag="VGP27_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852747.1"
                     /translation="GTESTPLTGPAKDLLVTKDADLLDSTGGVENKYYARGVGLILTV
                     QVSGPSERDQAIAVEKF"
     gene            complement(561..761)
                     /locus_tag="VGP27_15545"
     CDS             complement(561..761)
                     /locus_tag="VGP27_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852748.1"
                     /translation="MRELSVAEQRYQGRGVSRWFLAALENRWSPAERLDAMWDGHCSA
                     GPMFSGGGWGAVPPASLAAVVR"
     gene            889..1305
                     /locus_tag="VGP27_15550"
                     /pseudo
     CDS             889..1305
                     /locus_tag="VGP27_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1725..2960)
                     /locus_tag="VGP27_15555"
     CDS             complement(1725..2960)
                     /locus_tag="VGP27_15555"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7852749.1"
                     /translation="MDNATIFTLTVMVLCYAVISALVKRWYVAPALIFVLCGMALGPF
                     GLGAIKVGNEVNNFLLPAELALTLILFNQASALDLSAVVRRGGITFRLLVIGIPLSLG
                     LGTLTGALLIPIMPLWEAVAMAAIVAPAEVALIAALLEDRRIPEPVRQALSTESGFYD
                     GFALTTMLAALAVAHEQERTHAGLDELAWFLVRTEVMSVVVGLILGAIGGAVVTWSRQ
                     RGWMSDTWVQLSMLTGALLCFEVGGQLDGSGLVAAFAGGLGFAFVAKRKGGRPDTHVS
                     DAAAHLLELVIFAMFGAYAVVEAWRDVGWQVVVFAFVALFGVRLIAVLVALIRTDLPT
                     RDRLFIGWFGPRGLGTIVLGLLMVEQTVPQREYWIMQAVGVTVSLSLFVHSLTVWPGI
                     NWLATKEPIEVGQIGSSPS"
     gene            complement(2970..3197)
                     /locus_tag="VGP27_15560"
     CDS             complement(2970..3197)
                     /locus_tag="VGP27_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852750.1"
                     /translation="MSRCGRHFITKLLDVPGDAVSDLYTKARRNDGRNTLVTQAKSGP
                     AVWPHDTYSSTVDLAYPFIWDLTSGGPTDGA"
     gene            3480..4211
                     /locus_tag="VGP27_15565"
     CDS             3480..4211
                     /locus_tag="VGP27_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852751.1"
                     /translation="MWLLLAATPLVLLVTYYVSFMTHEYGHSFAAWILGIKSSPWPIH
                     WGSNSIGNILFLDEVDEHVDYDTAMASGKNLAVAVVAVSGIAVNALLYFVFRFAAPLW
                     RTGTRRLVAYSTFWLLCMEVGNFYCYVPIRVASPSGDMHNWTVGSHQSWWWLYVIIGY
                     LTLWAMIDFYRTVLPASLDASGIGTRVGRALVLVTATLLLFGYFAGPGLLKDDDLTQF
                     ISRTSLLLIPIVLLASWGRVVRTAH"
     gene            complement(4244..4369)
                     /locus_tag="VGP27_15570"
     CDS             complement(4244..4369)
                     /locus_tag="VGP27_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852752.1"
                     /translation="MVYVVGNLVLLFDLLLPWLGVLAVAGGIASGVIRLARGRRP"
     gene            5241..5498
                     /locus_tag="VGP27_15575"
                     /pseudo
     CDS             5241..5498
                     /locus_tag="VGP27_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5542..5615)
                     /locus_tag="VGP27_15580"
     tRNA            complement(5542..5615)
                     /locus_tag="VGP27_15580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5579..5581),aa:Ile,seq:cat)
BASE COUNT         1144 a         1831 c         1670 g         1036 t
ORIGIN      
        1 ccggcaccga gtccacccca ctcaccggac cggccaagga tctgctggtc accaaggacg
       61 ccgacctgct cgattccacc ggcggggtcg agaacaagta ttacgcccgc ggcgtcggac
      121 tgatcctcac cgtgcaagtc agcggtccct cggagcgcga tcaagcaatc gccgtcgaga
      181 agttctgaac cccgatatgc gagctgaacc ggaaccggtg attcccggtg ggctaagcgc
      241 ggtgtcggga cgaacgactc cgcatcatgc acagcaagct ctctcgtcgg gtcgggcccc
      301 cagacccgtc aactcagtgt tggctgaggc gatcgcactc accgccgtag ggatgtcagc
      361 agtgggcgtc attgtgtccg cggttctcgt ggtggcccgc gggccagccg accttaccgg
      421 cgtcgtcgtt cccgtcattc cgatcaccac caccaccgcg gcacccgtgc tcccgcctcc
      481 gccaaccgcg tcggcgccta cgcaggcgcc ctcacgcatt ccggcgagca cgccgccagc
      541 gatcatcacc accgtggcac ctagcgcacc accgccgcca gcgaagccgg cggaaccgcg
      601 ccccacccgc cccccgagaa catcggcccc gccgagcagt gtccatccca cattgcatcg
      661 agcctttccg caggagacca ccgattttcc agggccgcca ggaaccaacg gctgacgcca
      721 cggccctggt accgctgctc ggccacgctc aactccctca tctagggagt gtcaaggatc
      781 aaccgaagta gatgtaaagc atcagccaaa acagtgtcag ccaacacccg aagaccacac
      841 gtcaaccatc aggcgacgta atacaacttt ctaggtgggg cgggcgggga ctgggatcag
      901 atcgccaagg acatccgccc ggtctacacc gcgccgaccg aggccgcggc caaggaacgc
      961 ttcgttgaat tcaccgcgaa atggggtgcc cagtacccgg caatcatccg gctctgggag
     1021 aacgcctgga gcgagttcgt gccgttcctg gactacgacg tggagatacg ccgagtcatt
     1081 tgttcaacga acgccgtgga gtcgatcaac gcccgctatc ggcgcgcgat ccgcgcccgc
     1141 gggcacttcc caaccgaaca ggctgccctc aagtgtctct acctcgtgac tcaatcactg
     1201 gatccgactc ggcgaggtag ggcacgatgg gcaatgagtt ggaagccggc gctcaatgcg
     1261 ttcgcgatca ccttcaacgg ccgaatcacc ccgacctgcc actagaaatg ccaaggtcgg
     1321 atccaccgtt aatcggacag ccccgatcaa ggtgtaaacg atgtctcggg acacctgtca
     1381 acgatgtccc gagacatcac atggtcgggc tgacagaatt tgaacctgcg accacttgac
     1441 ccccaaggga gcagcggtag ctcgttttcg caggtcgata ctttccgcgg gcagggcgcg
     1501 gtgtgtgacg ttgagggacg cgcagttcgt tcagcatcgt gcagagcgtg tggtcccaga
     1561 ctggtcccgg gcagaggggt gctcgtgtcg ggtggtcgtg tcgcgtcgag ttccacgagg
     1621 gtgatcgtcg cgccctcggc cgagtgcctt gtgtaaaggc gcctcgggac caccacttag
     1681 aactacgctt ctcgacgcag caaaagggcc tgtcgtcttc caggtcagga cggcgacgag
     1741 ccgatctggc ctacctcgat gggctctttc gtggcgagcc agttgatgcc gggccacacg
     1801 gtgaggctgt gcacgaagag gctcaacgag accgtgacgc cgaccgcttg catgatccag
     1861 tactcccgtt ggggcacagt ttgttcgacc atcaacaggc ctaggacgat ggtgccgagg
     1921 ccacgtgggc cgaaccaacc gatgaacagc cgatctcttg tcggcaagtc ggtacgaatc
     1981 agcgcgacca agacggcgat gagacgcacc ccgaacagcg cgacaaatgc gaagacgacg
     2041 acctgccatc cgacgtcgcg ccatgcctcg accaccgcgt aggcgccgaa catcgcgaaa
     2101 atcaccagtt ccagcagatg agcggcggca tctgagacgt gcgtatcggg gcgaccccct
     2161 ttccgtttgg ccacgaaggc gaacccgagg ccacctgcga acgcggcgac cagcccgctg
     2221 ccgtccaact gtccaccgac ctcgaagcag agcaacgcac ccgtcagcat ggacagttga
     2281 acccacgtgt cgctcatcca gccacgctga cgtgaccaag tgaccaccgc gcccccgatc
     2341 gcgcccagga tcagacccac caccaccgac atcacttcgg tacggaccag gaaccacgcc
     2401 aactcatcga gtccggcatg ggtccgttcc tgttcatgcg ccaccgccaa cgcggcgagc
     2461 atggtggtca gggcgaatcc gtcgtagaag ccgctctcgg tggacaacgc ttgtcgaacg
     2521 ggctcgggga tgcggcggtc ctcgaggagt gcagcgatga gtgccacctc cgccggggcc
     2581 acgatcgccg ccattgcgac cgcctcccac aacggcatga tcggaatcag gagtgcgccg
     2641 gtgagggtgc ccagcccgag tgacagcggt atcccgatca ccagcagccg gaatgtgatg
     2701 ccgccgcgtc gcactaccgc cgataagtcg agagcggacg cttgattgaa caggatcaac
     2761 gtcagcgcca gctcggcggg gagtaagaag ttgttcactt cattgcccac cttgatcgca
     2821 ccgaggccga acggccccaa ggccattccg cacagcacga atatcaaagc gggcgccacg
     2881 taccaccgtt tgaccaacgc cgagatcacc gcgtagcaca acaccatgac ggtcaaggtg
     2941 aatatggtgg cgttatccat ctgtggtcgt caggccccgt cggtggggcc acccgaggtc
     3001 aggtcccaga tgaacggata agccagatcg actgtgctgg agtaggtgtc gtggggccac
     3061 acagctggtc ccgacttggc ttgtgtcacc aacgtattgc gtccgtcgtt tcgtcgagct
     3121 ttcgtgtaca gatcgctgac agcgtcgccc ggtacgtcga gaagcttcgt gatgaaatgt
     3181 cgtccgcagc gtgacatggc gaagacggta ctacgcttgt gcctgcgttg agggccaacg
     3241 aggtgaactc cacgacgtgc gccgtgcagc tgattcagcg ctaaccgaac gaatccgtaa
     3301 acctcaaggg acgttgccgc gcacacgcct atgcttttcg caacggacgg ccggcagccg
     3361 ctgcgatcgc ctaaacggtt ggagagcgtg tgctagagga cccgactacc cgacgctcac
     3421 catctgaccc gaggccgatg ggtgacccac gagccgacga atcagccagt tcccgatcga
     3481 tgtggctgct gctggcggcc acgccgctag tcctcctcgt tacctactac gtgtccttca
     3541 tgactcacga atacggccac tccttcgccg cgtggatcct aggtattaaa tcgagtccgt
     3601 ggccaatcca ctggggcagc aacagcatcg gcaacatctt gtttcttgac gaagtagacg
     3661 aacacgtcga ctacgacact gcgatggcat ccggtaagaa tctggcagtc gctgtggtcg
     3721 cagtttctgg catcgcagtc aatgctctgc tgtacttcgt ctttcgtttc gccgcgcctt
     3781 tgtggcgcac aggtacgcgc cggctcgtcg cttactcgac gttctggttg ctgtgcatgg
     3841 aggtcggcaa tttctactgt tacgtcccta ttcgagtcgc ctcgccgagt ggcgacatgc
     3901 acaactggac cgtgggatca catcagtcgt ggtggtggct ttacgtgatc atcggatacc
     3961 tgacgttatg ggcgatgatc gacttctacc gaactgtgct gcctgcgtcg ctcgatgcca
     4021 gtggcatcgg gacccgggtt ggccgggcgc tggtgttggt gacggccact ctacttctct
     4081 tcggctactt cgcgggaccc ggtctgctga aggacgatga cctcacacaa ttcatatccc
     4141 gaacgtcgct tctgctcatt cccatcgtcc ttttggccag ctgggggcgg gttgtgcgga
     4201 ctgcacactg atcgtaaggg gggcgacgct tgtcaaccgc aggctagggt cggcggcccc
     4261 ttgccagccg gatcacgccg gaggcgatac caccggcgac agccagcacg cccaaccagg
     4321 gaagcagcag gtcgaacagc agcaccaggt tgccgacgac gtaaaccaag gcgtcccaac
     4381 ctcgttcgac gtgccccaga aaacccttgt actcattggg tgcaggaccg ctgacctcct
     4441 cgacccctcg tcgatcgcga tttgatggcg tcgaccccgc gattgtcgta cgacttgctc
     4501 tgcggaatcg cacacgccga aggcctcctc aaactctgct catttcaacc taggccggag
     4561 cacacagcgg ggtctcgccc ctttgacgtc gctctcaaaa gtttcgtacg gcgtgggcct
     4621 gttcaggcct tccgcgaaaa gttcctgtaa ggccatatcg tcagtctcat cgaagtcgtc
     4681 ctcgacgacc acccggtacc tctccacaag agttcgcacg acaaaaagat ggagactccg
     4741 cacaaatccc cccaggtgtc tgggcaatga ggctgctgtg atgccgcaaa ctcggcgtgc
     4801 aggacagaag ttcaaggata cgcgtgacgc gcaaaagtcg acgatggtca acgttccctc
     4861 ggaagcacgt ggcgcagcta ttacgagact tctcccgctc atggattctg acacgccgct
     4921 gctggctatc catggattgg cgagagacct tacggctatt gatccgcgcc tttggtctgg
     4981 cgtggagcgg ctcatggtgc gtcacccggg tgcggagcga ggccgcggac ctctgcgagc
     5041 ggcacagtta gcgcctgtca acgacccaag caaatccaca ccgcagatta tcgcggtgga
     5101 taacgagttg ttagccccac cactctcggg tccgcttcaa tgcctgggcg gatcgcaaac
     5161 cggcggtgcc ggaggcaagt tcgtcacgtt gaccgtcgtc tcattgttcg tcacgttgtt
     5221 cgtcacttga tttgtgaccg ggctcgtggc gcgatcctaa caacttcggc aaggagtggc
     5281 gcacggtgcg cgaggcgttg ggtattccag aggtcaccac tcactcgttc cgtaagacgg
     5341 tcgccaccct gatcgacgac gaggggctgt cggcgcggat cggtgcttat catctcgggc
     5401 attccaaggt ttcgatgacc caggatcggt acatgactcg aggtcgggtc cacgccgagg
     5461 ttgcgaactt gttagaccgc gccataaagg acgaataaac gacgaccccg cgcttgcgtg
     5521 gctcaaaaat ggcctctgaa gtggggcggg cggggctcga acccgcgacc aatggattat
     5581 gagtccacgg ctctaaccaa ctgagctacc gcccccgatg tacggcctta gagactagcg
     5641 cccgccgcca tcgcacggcg atcaggtaac cgaccacgac a
//
LOCUS       DASYLX010000656         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3326202,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000656 DASYLX010000000
VERSION     DASYLX010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326202"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGP27_15585"
     CDS             <1..194
                     /locus_tag="VGP27_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV7852753.1"
                     /translation="RFSRRPVLPEGIHRDIEVITDMWGRPRVRLTGAIAEHLKDVTIH
                     LSLTHEADTAAAVAVLEER"
     gene            316..1650
                     /locus_tag="VGP27_15590"
     CDS             316..1650
                     /locus_tag="VGP27_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV7852754.1"
                     /translation="MSDLVERVRDVLPSVRRDLEDLVRIQSVGADPARRAEVQRSAEA
                     VANLLSEAGFGEVHIVSEGGAPAVIARHPAPSGAPTVLLYAHHDVQPEGDHAQWVSPP
                     FEPTERDGRLYGRGSADDKAGIATHLAAFRAHGGQPPVGVTVFVEGEEETGSPSLGRL
                     LAAHKDTLAADVIVIADSDNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGLWGGVV
                     PDALSVLVRLLASLHDDDGNVAVEGLHEATAADVDYPKDRVREESGMLTGVAEIGSGS
                     VPQRLWAKPAITVIGIDTTPIASSSNTLIPRARAKVSMRVAPGGDARAHLEALTRHLN
                     KHVPWGAQLTVTPGDLGQPYAIDASGPVYEAARDAFRQAWGAEPVDMGMGGSIPFIAE
                     FADAFPSAKILVTGVEDPGTQAHSINESLHLGVLERAATAEALLLANLASRT"
     gene            complement(1665..2138)
                     /gene="bcp"
                     /locus_tag="VGP27_15595"
     CDS             complement(1665..2138)
                     /gene="bcp"
                     /locus_tag="VGP27_15595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEV7852755.1"
                     /translation="MAQTPRLEAGDKAPAFSLLDADGNTVSLADYRGRKVIVYFYPAA
                     STPGCTKEACDFRDNLGELNEAGLDVVGISPDKPQKLAKFRDNEKLTFPLLSDPDRKV
                     LTEWGAFGEKTMYGKTVQGVIRSTFVIDEKGKIDVAQYNIKATGHVAKLRRDLSV"
BASE COUNT          328 a          697 c          776 g          367 t
ORIGIN      
        1 cccggttctc caggcggccg gtgctccctg agggcatcca tcgcgacatc gaggtcatca
       61 ccgacatgtg gggtaggccg cgggtgcggc tgaccggtgc gatcgccgag catttgaagg
      121 atgtgacgat tcacctctcg ctgacccacg aagccgatac cgcggcggcc gtcgccgtcc
      181 tcgaggagcg ttaacctcgc cccgtgagct gggcacctcg ccccgcgagc gcgcgtgttt
      241 gtacacgaca cgccgcgctt ttctggcatt ctctgcacgc tcgcggtgca gacctgagcg
      301 gctagcctcg aaaccatgag cgatctcgtg gaacgggtac gtgacgtgct gccgtcggtg
      361 cgacgcgacc tcgaagacct tgtgcgtatc cagtcggtgg gcgccgaccc ggcgcggcga
      421 gccgaggtgc agcgcagcgc cgaggcggtg gcaaacctgt tgtcggaagc cgggttcggt
      481 gaggtgcaca tcgtcagcga gggcggcgca ccggccgtca tcgcgcgaca tcctgcgcct
      541 tcgggcgcgc cgacggtgct gctgtacgca catcacgatg tgcagcccga gggcgaccac
      601 gctcaatggg tgtcgccgcc cttcgagccg accgagcgtg acgggcgcct ctacggccgc
      661 ggcagtgccg acgacaaggc cggcatcgca acgcatctcg ccgcattccg ggcgcacggt
      721 ggtcagccgc cggtcggtgt gacggtgttc gtcgaaggcg aggaggagac cggctcgccg
      781 tcgctgggcc ggctgctggc cgcgcacaag gacacgctcg ccgccgacgt catcgtcatc
      841 gcagactcgg acaactggag caccgacgtt ccggcgttga cggtgtcgct gcgcggtctg
      901 gtcgactgcg ttgtggaggt ggccacgctc gaccacggtc tgcactcggg cctgtggggc
      961 ggtgtggtgc ccgacgcgct gagcgtgctg gtgcggttgc tggcgtcgct gcacgacgac
     1021 gacggcaatg tcgccgtaga gggtctgcac gaggcgaccg cggccgacgt ggactatccg
     1081 aaggaccggg tgcgcgaaga atcgggaatg ttgaccggtg tcgctgagat cggctccggg
     1141 tcggtgccgc aacggctttg ggccaaaccg gcgatcacgg tcatcggaat cgacaccacg
     1201 ccgatcgcgt cgtcgtcgaa cacgctcatt ccgcgggcgc gggcgaaggt cagcatgcgg
     1261 gtagcgccgg gcggcgacgc gcgtgcccat ctcgaggcgt tgacccgtca cctgaacaag
     1321 cacgtgccgt ggggcgcgca gctcaccgtc acccccggcg acctcgggca gccgtacgcg
     1381 atcgacgcga gcggacccgt ctacgaggcg gcgcgcgatg cgttccggca ggcctggggc
     1441 gccgagccgg tggatatggg catgggcggg tcgatcccgt tcatcgccga gttcgccgat
     1501 gcgttcccgt cggcgaaaat cctggtcacc ggtgtggaag accctgggac acaagcgcat
     1561 agcatcaacg agagcctgca tctcggggtg ctggagcgtg ccgcgacggc cgaagcgctg
     1621 ctgctggcga acctggcgtc gcggacgtga ggaggagaga gagatcagac cgatagatcg
     1681 cgacgcagct tggccacgtg gccggtggcc ttgatgttgt actgcgcgac gtcgatcttg
     1741 cccttctcgt cgatgacgaa cgtggaccgg atgacgccct gcacggtctt gccgtacatc
     1801 gtcttctcgc cgaaggcgcc ccactccgtc agcaccttgc ggtccgggtc ggacaacagc
     1861 gggaaggtga gcttctcgtt gtcgcggaac ttggccagct tctgcggctt gtcgggggag
     1921 atgccgacga cgtcgaggcc ggcttcgttc agttcgccca ggttgtcgcg gaagtcgcac
     1981 gcctccttgg tacatccggg cgtcgacgcc gccggataga agtagacgat gactttgcgg
     2041 ccgcggtagt cggccagcga caccgtgttg ccgtcggcgt cgagcaggct gaacgcgggg
     2101 gctttgtcgc ccgcctccag gcgtggggtc tgtgccaccg gtgattcctt ctctccaccg
     2161 gtgcgtcg
//
LOCUS       DASYLX010000657         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_362702,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000657 DASYLX010000000
VERSION     DASYLX010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362702"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /gene="dnaK"
                     /locus_tag="VGP27_15600"
     CDS             complement(<1..1245)
                     /gene="dnaK"
                     /locus_tag="VGP27_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV7852756.1"
                     /translation="MATAVGIDLGTTNSVIAAWQGGEPVVIPNAEGARTTPSVVAFTE
                     NGERLVGQLARRQSIMNPKGTIYSAKRFIGRRFDEVSDEAKAVSFDVVPGPNGEARFE
                     IRGKQYSPEEISALILRKLVDDAGKFLGERVKEAVITVPAYFNDAQRNATKDAGRIAG
                     LDVLRIINEPTAAALAYGMDKKGHETVLVFDLGGGTFDVSLLDVGDGVVEVRATAGDS
                     HLGGDDFDRRLVDYLADEFQRENNIDLRKDPQALQRLFEAAEKAKVELSSVAQTQVNL
                     PFITADANGPKHLTMTINRSKFEELTHDLVERTMGPVKQAMSDAKVTANDIDEVILVG
                     GSTRIPAVQALVRRLTGGKDPNMSVNPDEVVALGAAIQAGVLKGEVSDVLLLDVTPLS
                     LGVETAGGLMTKIIERNTTIPVR"
     gene            complement(1249..1755)
                     /locus_tag="VGP27_15605"
     CDS             complement(1249..1755)
                     /locus_tag="VGP27_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEV7852757.1"
                     /translation="MAAHSTVETSAVRPNEPPRQVIATFDNYADAERVVDYLSDRQFE
                     VDRLAIVGRDMEYHEQVLGRLNYGGAALRGAGSGALVGLLIGWIFGLFNWIQPLVSAL
                     VLAGYGLVFGAVVGALVGLLAHALQRGRRDFYSVSGLRPKYYDVLADVAVADRAIRLL
                     GGATDRRE"
     gene            complement(1755..>2304)
                     /locus_tag="VGP27_15610"
     CDS             complement(1755..>2304)
                     /locus_tag="VGP27_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7852758.1"
                     /translation="HWRAVLTGDRLPELSGTATLETEGDPMALMRFDPFRELDRLADQ
                     ALAGARSATHALPMEALRRGDQFIVALDAPGMQRGDIDVTVERNVVEITGRRRPIRQE
                     GDELIVDERPQGEFRRQLFLGDNLDPNKMTAVCDLGVLTLTIPVSQESKPRKVEIAEP
                     NQGQQAIQPETRTPQTADAAVQ"
BASE COUNT          385 a          747 c          735 g          437 t
ORIGIN      
        1 ccgtaccggg atggtggtgt tgcgctcgat gatcttggtc atcagcccgc cggcggtctc
       61 cacacccagc gacaacgggg tgacgtccag cagcaagacg tcggagacct cgcccttgag
      121 cacaccggcc tggatcgcgg caccgagcgc gacgacctcg tcggggttca cgctcatgtt
      181 ggggtccttg ccgccggtga gccggcgcac caaggcctgg actgcgggga tccgggtaga
      241 accgcccacc aggatgacct cgtcgatgtc gttggccgtc actttggcgt cgctcatggc
      301 ctgcttgacc gggcccatgg tgcgctccac cagatcgtgc gtgagttcct cgaacttcga
      361 ccgattgatg gtcatggtga gatgcttggg gccgttggcg tcggcggtga taaacggcag
      421 attgacctgg gtctgcgcca ccgacgacag ttcgaccttg gccttctcgg ccgcctcgaa
      481 cagccgctgc agcgcctgtg ggtctttccg taaatcgatg ttgttctcac gctggaactc
      541 atcggcgagg tagtcgacga gccggcggtc gaagtcatcg ccacccaggt gcgaatcacc
      601 cgccgtggcg cggacctcga ccaccccatc acccacgtcg agcaggctga cgtcgaacgt
      661 gccgccgccg aggtcgaaga cgagcaccgt ctcgtgcccc ttcttgtcca tcccgtaggc
      721 cagtgccgcg gccgtaggct cgttgatgat gcgcagtacg tcgaggccgg cgatccggcc
      781 ggcatccttg gtggcgttgc gttgggcgtc gttgaaatag gccgggacgg tgatcaccgc
      841 ttccttgacc cgttcaccga ggaacttgcc ggcgtcgtcg acgagtttgc gcaagatcag
      901 cgcgctgatc tcctcaggcg aatactgctt gccgcgtatt tcgaaacgtg cctcaccgtt
      961 gggaccggga acgacgtcga agctgaccgc cttggcttca tctgatacct cgtcgaagcg
     1021 gcggccgatg aatcgctttg ccgaatagat ggtgcccttc gggttcatga tcgactggcg
     1081 cctggccagt tgcccgacca ggcgctcgcc attttccgtg aacgcaacca cagagggcgt
     1141 ggtgcgcgcc ccctcggcgt tcgggatcac gaccggttcc ccgccctgcc aggcggcgat
     1201 taccgagttg gttgtgccga gatcgattcc tactgctgtg gccatgacct attcccttct
     1261 gtcggtggcc ccgccgagca gtcgaatggc acggtcggcc accgccacgt cggcgaggac
     1321 gtcgtagtac ttgggccgca gtccgctgac cgagtagaag tcccggcggc cgcgttgcag
     1381 ggcgtgggcg agcagtccga ccagggcgcc cacgacggcg ccgaagacca atccatagcc
     1441 ggccagcacc agggcggaga ccaatggttg gatccaattg aacagcccga agatccaccc
     1501 gatcagcagg ccgacaaggg cgccggaccc ggcgccacgc agggcggcgc caccgtagtt
     1561 cagccggcca aggacttgtt cgtggtactc catgtcccgg ccgactatgg ccagcctgtc
     1621 tacctcgaat tgccgatcgg acaggtagtc gaccacccgt tcggcgtcgg cgtagttatc
     1681 gaaggtggcg atgacctggc ggggcggctc gttcggccgg accgccgagg tttccaccgt
     1741 tgaatgcgca gccatcactg caccgccgca tccgcggttt gtggcgtcct tgtttcgggt
     1801 tgaatggcct gctggccctg gttgggttcg gcgatctcaa ccttgcgtgg cttgctctcc
     1861 tgcgacaccg gaatggtcaa cgtcagcacg cctagatcac atacagcggt catcttgttc
     1921 ggatcgaggt tgtcaccgag aaacagttgg cgacggaact cgccctgcgg ccgctcatca
     1981 acgatcagct catcgccctc ctgtcgaatc gggcggcggc ggccggtgat ctcgacgacg
     2041 ttgcgctcga ccgttacatc gatatcgcca cgctgcatgc ccggtgcatc aagggcgaca
     2101 atgaattggt caccgcgacg caacgcctcc atgggaagag cgtgcgtcgc actgcgagcg
     2161 ccggccaggg cctggtcggc cagccggtcg agttcgcgaa acggatcgaa tcgcatcaaa
     2221 gccatgggat ctccttcggt ttccagggtt gctgtaccgg aaagctccgg cagccgatcg
     2281 cccgtaagca cggcccgcca gtgc
//
LOCUS       DASYLX010000658         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3326389,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000658 DASYLX010000000
VERSION     DASYLX010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326389"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VGP27_15615"
     CDS             complement(<1..976)
                     /locus_tag="VGP27_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852759.1"
                     /translation="MLKYRGTKLVRTGFIGVVLAILIILVGLSPETLTSMATAVRYQA
                     QFSDAGGLTAGNDVTIAGIKVGSVSGVSLQGRNALVTFAIDSTVELGADTTAHVRTGT
                     LLGERVLTLESAGGDTMHPMDVIPVSRTASPYSLTEAVSELTTNTAGTDTASLNQSLD
                     TLSDTLNQVAPQLGPTFDGVTRLSKALNERDDTLGELLKDGADVTKILSDRSAQVNTL
                     ILNANDLVGVLNDRRYAIVELLVHTSALSKQLSGLIHDNEEKLAPTLDKLNAVTAVLE
                     KNRDNIGKALPGLAKFQTTLGETIGNGPYYQAYVPNLFLSEIFQPFFDY"
     gene            complement(976..2001)
                     /locus_tag="VGP27_15620"
     CDS             complement(976..2001)
                     /locus_tag="VGP27_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852760.1"
                     /translation="MRRSLRPTLVKFGVFGVVMVVLTACLFFIFGQYQTGSTKDYSAV
                     FTDASRLKTGQSVRVAGMRVGTVKGVELQKDKTALVTFDADPAVQMTTGTHVAVHYLN
                     LVGDRYLELIDGPGDRLLPPGSRLPAERTAPALDLDLLLGGLKPVIRGLNPSDVNALS
                     ASLIQIFQGQGGTLQSLFSNTSGFTTAMAEHNQTIQALIDNLRTLLSTLTKEGDRFSG
                     TIDKLEKLVTQLAAERDPIGSAIESLNNGTASVADLLTEARPPLKGTVDQLNRLATNM
                     DGQKDRLDTALQKAPENYRKLIRTGSYGSFFNYYICQLTWRVTDLQGRTAEFPWIRQD
                     GGRCTEP"
     gene            complement(1998..>2936)
                     /locus_tag="VGP27_15625"
     CDS             complement(1998..>2936)
                     /locus_tag="VGP27_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852761.1"
                     /translation="DPKRMSAIPANVLVDIASTTVFGAKYVQLVFPPDPSPESLRAGQ
                     VLAAKHVMVEINTVFEQLTSVLSRIDPPKLNETLGAIASSVNGRGEKIGQMLTDLDAY
                     LARIEPSLPALSHDLETAPQVLAAYGDAAPDFVTIADNAARFSDTLVDQQQNLDAALV
                     SVIGLADIGNDVIGTNRQPLTDVLHLLVPTTDLLNKYNQALWCSLSGMVNTAARPPLK
                     EPGVVVMAGFLWGQERYRYPMDLPKAGATGGPQCTGLPNLPFEGVPPYVVADTGTNPW
                     RRTYPGIVLNSDGLKKILFGEIDGPPRNTYQIGQPG"
BASE COUNT          498 a          869 c          984 g          585 t
ORIGIN      
        1 cgtaatcgaa gaacggctgg aagatctcgc tgaggaacag attcgggacg taggcctggt
       61 agtagggtcc gttgccgatc gtctcgccga gagtagtctg aaacttcgcc agccccggca
      121 gtgccttgcc gatgttgtcg cgattcttct ccagcacggc tgtgaccgcg ttcagcttgt
      181 caagtgtcgg cgccagcttc tcctcgttgt cgtggatgag gcccgacagc tgcttcgaca
      241 atgccgatgt gtggacgagc agctcgacga tcgcataccg ccggtcgttg agcacgccca
      301 ccaggtcgtt ggcattgagg atcagggtgt tcacctgtgc acttcggtcc gacaggatct
      361 tggtgacatc ggcgccgtcc ttgagcagct cccctagcgt gtcgtcgcgt tcattgagcg
      421 ccttggacag ccgcgtgacg ccgtcgaatg tcgggccgag ttgtggcgca acttgattca
      481 gggtgtccga cagggtgtcc aacgattgat tgagcgatgc ggtgtcggta cctgcggtgt
      541 tggtggtgag ttcgctgacc gcctcggtca gcgaatacgg cgaggctgtg cgcgaaaccg
      601 ggatgacgtc catcggatgc atggtgtcgc cgcctgcgga ctccaaggtg agcacacgtt
      661 caccgaggag cgttccggtt cgcacgtgcg ccgtcgtgtc cgcgccgagc tcgaccgtgc
      721 tgtcgatggc gaacgtgacc agcgcgttgc gtccctgcag cgacacaccg gagacgctgc
      781 cgaccttgat tccggcgatc gtcacgtcgt tgcccgcagt caggccgccg gcatcggaga
      841 actgggcctg ataacggacc gccgtcgcca tcgacgtcaa cgtctccggg gaaagcccga
      901 cgaggatgat gagtatcgcc aggacgacgc cgatgaagcc cgtccggacc aattttgtgc
      961 cacggtattt cagcatcagg gctcggtgca cctcccgccg tcctgcctga tccacgggaa
     1021 ctcggcggta cggccctgaa ggtctgtcac ccgccaggtg agctggcaga tgtagtagtt
     1081 gaagaagctg ccgtacgaac ctgtgcggat cagcttgcgg tagttctcag gcgccttctg
     1141 cagtgcggtg tcgagccggt ctttttggcc gtccatgttg gtggccagcc tgttcaattg
     1201 gtcgacggtg cccttcagtg gcggtcgcgc ttcggtgagc aggtcggcga ccgatgcggt
     1261 gccattgttc agcgactcga tggctgaccc gatcggatcc cgttctgcgg caagctgggt
     1321 cacgagtttc tccagcttgt cgatcgtgcc ggagaaccgg tcgccctctt tggtcagggt
     1381 ggacagcaac gtgcggaggt tgtcgatcag cgcctggatc gtctgattgt gttccgccat
     1441 cgccgtggtg aaacccgagg tgttggagaa cagggactga agtgtgccgc cctgcccttg
     1501 gaagatttgg atcagcgacg ccgacagcgc attgacatct gacggattaa ggccgcggat
     1561 aacgggtttc agaccgccga gcaacaggtc gagatccagc gcgggggccg tccgctcagc
     1621 gggaaggcgg gacccgggcg gcaacagccg gtcccccggg ccgtcgatca actcgaggta
     1681 gcgatcgccg acgagattga ggtagtggac cgcgacgtgg gtgcctgtgg tcatctggac
     1741 ggcggggtcg gcgtcgaagg tgacgagcgc ggtcttgtcc ttctgtagtt cgacgccttt
     1801 gacggtgcct acccgcatac cggccacccg gaccgactgg ccggttttga ggcgcgaggc
     1861 gtcggtgaac accgcggagt agtccttcgt cgaaccggtt tggtactgcc cgaagatgaa
     1921 gaacaggcat gcggtcaaga ccaccatcac gacgccgaag acgccgaact tgaccagagt
     1981 gggacgtagt gagcgcctca tccgggctgt ccgatctgat aggtgttgcg gggcggcccg
     2041 tcgatctcgc cgaacaggat cttcttcaaa ccgtcggagt tcagcacgat cccgggatac
     2101 gtgcgccgcc acggattggt accggtgtcc gccacgacgt agggaggcac tccctcgaac
     2161 ggcaggttcg gcaggccggt gcactgcgga ccgcccgtgg cgcccgcctt cggtaaatcc
     2221 atcggatagc ggtagcgctc ctggccccac aggaagccgg ccatcaccac gacgcccggt
     2281 tccttcagcg gtggacgcgc cgcggtattg accattccgc tgagggagca ccagagcgcc
     2341 tggttgtact tgttgagcag gtcggtggtc ggcaccagca gatgcaacac atcggtcagc
     2401 ggctgccgat tggtgccgat cacgtcgttg ccgatatcgg caaggccgat aacactgacg
     2461 agtgcggcgt cgagattctg ctgttggtcc acgagcgtgt cgctgaaccg cgccgcgttg
     2521 tcggcgatgg tgacgaagtc cggcgcggcg tctccatagg ccgccagcac ctgcggggcg
     2581 gtttccaagt cgtggctcaa cgcgggcagg ctcggttcga tcctggcgag gtacgcatcg
     2641 agatcggtga gcatctggcc gatcttctcg ccgcgtccgt tcacggatga cgcgatggcg
     2701 ccgagcgttt cgttcagctt cggcgggtcg atccgcgaca gcaccgacgt gagttgttcg
     2761 aaaactgtgt tgatttccac catgacgtgt ttggccgcga gcacctgacc ggcgcgcagc
     2821 gattcgggcg acggatccgg cgggaagacg agctggacgt atttcgcgcc gaacaccgtc
     2881 gtcgacgcga tgtcgacgag cacgttcgcc ggtatcgcac tcattcgttt ggggtc
//
LOCUS       DASYLX010000659         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_912554,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000659 DASYLX010000000
VERSION     DASYLX010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912554"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VGP27_15630"
     CDS             <1..1373
                     /locus_tag="VGP27_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7852762.1"
                     /translation="IDAADYFHHIKSAMLRAQHRIMLIGWDLDARMTFERGSKMLPGP
                     NQLGAFLYWMLWKRPELEVYLLKSNLRLLPAFDGIWYGLTPVSVVNQISSRRMHFAID
                     GARPTGAVHHQKIAVIDDAVAFCGGLDLTVDRWDTRAHEQQNRGRRTVGRSYGPRHDV
                     AAAVDGDAARALGEQACQRWEKATGRSVQPVDAKHIAWPRKLQPTLRDVDVGIARTLP
                     QLDDRDEVREVEALNLEAIAAARHTIYLENQYLASRRLVDALATRLSEEDGPEIVIVL
                     PRRGNNTLERGTMDGARHRLIRLLWAADEHHRLGVYYPVTEGGTPIYVHSKVMVVDDR
                     LLRIGSSNLNNRSMGFDSECDVAVEADTDSPDDDEVRRQITWVRDQLVSEHLGVSADE
                     FGEGRSLINTVEELRGEGRTLAPFTEETVADEASPLAENDLMDPDHVPRSLTRSVQRF
                     IEGLRR"
     gene            complement(1392..1715)
                     /locus_tag="VGP27_15635"
     CDS             complement(1392..1715)
                     /locus_tag="VGP27_15635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7852763.1"
                     /translation="MITFIAHFTVPPTNAAAFEELLTYVATMSNTEPGVVYYGFARSV
                     DEFDTYSVVEVYRDHEAVASHGETEWVTESVPRFLGLIEGLPQIKQYVSPGAAAVASR
                     FDDLT"
     gene            complement(1724..2653)
                     /locus_tag="VGP27_15640"
     CDS             complement(1724..2653)
                     /locus_tag="VGP27_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852764.1"
                     /translation="MTALSGRRVLVTGASGVVAYPVAVELAKNNEVFAVARFSDPEQS
                     RSLERAGAHPIPFDLADPDLSPLPESVDVVINYAVLPPTDKDAYELNAGATGRLARRY
                     RECEAFVHGSTGSLYAYQGERPLREDDPYGLHSSAENYAASKIAAEFLVKHLSADYQL
                     PAVIVRIFSFYGPRGGGVTQRIDQIVRGEPVSVYPGVRNVHTPLYEADYVEKTIAAAS
                     IATVGAEVVNVGGTEPVTTQEYCQMAGEILGREPVFTENSKAWPIWADTTKMVDLLGP
                     NKVTVREGVRRTIEAAQNRLNTSHHVIGEPAKA"
     gene            complement(2650..>3136)
                     /locus_tag="VGP27_15645"
     CDS             complement(2650..>3136)
                     /locus_tag="VGP27_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7852765.1"
                     /translation="KARYCRYLDAKDWVAWRELFADDFTSVIAGAGGRQTVGADDFVV
                     YVRRTLGKSSQPTVHQVYAPEITLTSPTTATGVWALNDVVRLIPAVTLHGYGHYHETY
                     EKSETHWRIKTSTVTRLREDITTPLPQGIATRLRVAAAKLARRTSTVANVSTDGGKRF
                     T"
BASE COUNT          539 a          978 c         1049 g          570 t
ORIGIN      
        1 tcatcgatgc ggccgactac ttccaccaca tcaagtcggc aatgctgcgg gcccagcacc
       61 ggatcatgct gatcggctgg gatctcgacg ccaggatgac cttcgagcgt gggtccaaga
      121 tgctgcccgg gcccaaccag ctgggcgcgt tcctttattg gatgctctgg aagcggccgg
      181 agctcgaggt ctatctgttg aagtcgaatc tgcggctgct gccggccttc gacggcatct
      241 ggtatgggct cacgcctgtg tcggtggtga atcagatcag cagcaggcga atgcacttcg
      301 cgatcgatgg ggcccggccg accggagccg ttcaccacca gaagatcgcg gtgatcgacg
      361 atgccgtggc gttctgcgga ggcctcgacc tcaccgtcga tcggtgggac acccgtgcgc
      421 acgagcagca aaaccgtggc cgtcggacag tggggcggag ctacggtcca cggcacgacg
      481 ttgcggctgc agtcgacggt gacgccgcgc gcgcactcgg tgaacaggcc tgccagcggt
      541 gggagaaagc gacgggccga tctgtgcaac ccgttgacgc gaagcacatt gcgtggcccc
      601 gcaagctgca acccactctg cgcgacgtcg acgtcggaat cgcgcgcacc ctgccgcaac
      661 tcgatgatcg cgacgaagtc cgcgaagtgg aggcgctcaa cctcgaagcc attgcggcag
      721 cacgccacac catctacctg gagaaccaat acctggcctc gcggcgcctg gtcgatgcgc
      781 tggccactcg actgagtgag gaagacggtc cggagatcgt catcgtcctc cctcgcagag
      841 ggaacaacac cctcgagcgg ggaacgatgg acggcgcgcg tcaccgcctc attcgactgc
      901 tgtgggccgc cgacgagcac catcgattgg gcgtctacta cccggtgacc gagggcggca
      961 cgccgatcta cgtccactcg aaggtgatgg tcgtcgacga ccggctgctg cggatcggct
     1021 catcgaacct caacaaccgg tcgatgggct tcgacagcga gtgcgacgtg gccgtcgagg
     1081 cggacacgga cagcccggat gacgacgagg tccggcgcca gatcacctgg gtgcgtgatc
     1141 aattggtgtc ggaacatctg ggcgtgtccg ccgacgagtt cggcgaaggc cggtcgctga
     1201 tcaacaccgt cgaagaactg cggggtgagg gcaggacgct ggcgcccttc accgaagaga
     1261 ccgtggccga cgaggcgagt ccgctggccg agaacgacct gatggatccc gaccacgtgc
     1321 cgcggtcgct gacgcggagc gtgcagcggt tcatcgaggg acttcggcgc tgacccagct
     1381 cgccgaacgc gtcacgtcag atcgtcgaac cgagacgcga ccgccgcagc gccggggctg
     1441 acgtactgct tgatctgtgg caggccttcg atgaggccca ggaaccgggg caccgattcg
     1501 gtgacccatt cggtctcacc atgcgaggcg acggcttcgt gatcgcggta cacctcgaca
     1561 acggaatacg tgtcgaattc gtcgacgctt cgagcgaagc cgtagtagac gacgccgggt
     1621 tcggtattgg acatcgtggc gacataggtc agcagctctt cgaatgccgc ggcgttggta
     1681 ggcggcaccg tgaagtgagc gatgaaggtg atcatctgcg cttctaggct ttcgcgggct
     1741 cgccgatgac gtgatgagag gtgttgagcc ggttctgcgc cgcttcgatc gtccggcgca
     1801 ccccttcgcg caccgtgacc ttgttgggcc cgagaagatc caccatcttg gtggtgtcgg
     1861 cccatatcgg ccaggccttg ctgttctcgg tgaaaacggg ttccctgccc agtatttcac
     1921 cggccatctg gcagtactcc tgcgtcgtca ccggctcggt tccgccgacg ttgacgacct
     1981 cggccccgac tgtggcgatg ctcgctgccg cgatggtctt ctcgacgtag tcggcttcgt
     2041 agaggggggt gtggacattg cgcacgcccg ggtagacgct caccggctcg ccgcgcacga
     2101 tctggtcgat gcgttgggtc acgccgccgc cccgtggtcc gtagaaggag aagatccgaa
     2161 cgatcacagc tggcaattga taatccgcgc tgagatgctt caccagaaac tcggccgcta
     2221 tcttgctcgc ggcgtagttc tccgccgagg agtggaggcc gtaggggtcg tcctcccgta
     2281 gcggacgctc accctgatac gcgtagagcg atccggtgct gccgtgcacg aaggcttcac
     2341 attcccggta ccgcctggcc agccgaccgg tggcgccggc attcagttca taggcgtcct
     2401 tgtcggtcgg tggcaggacc gcgtagttga tcaccacgtc aaccgattcg ggcaacggcg
     2461 acagatccgg atctgcgaga tcgaacggga tggggtgggc gcctgcccgc tcgaggctgc
     2521 ggctctgctc cggatcggag aaccgggcga ccgcaaagac ctcgttgttc ttggccagct
     2581 cgacggcgac cgggtaggcc accacgccgc tcgcgccggt gacgaggact cgtcgtcccg
     2641 aaagtgccgt catgtgaatc tctttccgcc gtcggtcgaa acattcgcca cagtcgacgt
     2701 gcggcgggcc agctttgccg cggcaacccg cagccgcgtg gcgatgcctt gcggcagtgg
     2761 tgtggtgatg tcttcgcgca ggcgggtcac ggtggaggtc ttgatgcgcc agtgtgtttc
     2821 gcttttctcg tatgtctcgt gatagtggcc gtagccgtgc agggtcactg ccgggatcag
     2881 gcgcacgacg tcgttgagcg cccacacgcc tgtcgcggtc gtcggcgagg tcaaggtgat
     2941 ctccggggcg tagacctggt gcaccgtggg ttgtgacgac ttgccgaggg ttcggcggac
     3001 gtagaccacg aagtcgtccg cgccgacggt ctgcctgcct ccggcgccgg cgatgacact
     3061 tgtgaagtcg tcggcgaaca gttcacgcca cgcgacccag tccttggcat cgagataccg
     3121 gcaatacctg gctttt
//
LOCUS       DASYLX010000660         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2338770,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000660 DASYLX010000000
VERSION     DASYLX010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338770"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2116)
                     /locus_tag="VGP27_15650"
     CDS             complement(<1..2116)
                     /locus_tag="VGP27_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7852766.1"
                     /translation="MSIRGGQLRLGLFAALLALLCVNACALWGDQTARWVFTALQAII
                     GLGAITCALVVARRKRGLTRGWRLVVVAGFLSSGAGDLLWYALRVTDDGTAPPPAVAL
                     YFMPPLLTLVIAVVLVRAGGPVTEEPEDPIRASRIITVLDGLVAAISFGMLVLMSGFG
                     DSTGAVLPRSENTLVLVAYAVIELVMVVTAVLTAIAYGANRLYRANFLLLACGALTIA
                     ASDRMVIYLRTVGHEGGHLWGSIGFLLGQLLIAYSVLDFAPRPAPGRGRSALDWVQLA
                     LPYIGFLGIASLYVFHLALGRRLNVIELYAAMVIITLVTARQVIAMRAQRRLMHRLYE
                     TQHRLAHQVHHDALTGLPNRVLFSKRLDEAIGAGAFVLIFVDLDDFKEVNDRFGHAGG
                     DELLCAVGERLQRCIGDADTLARIGGDEFAILISGEREAPEVVADRIRLAIRDPFAIH
                     GSSIRVRASMGLVRPDAEDPPPTSDDLLRQADVSMYAGKRVGKDTAVIYRPSLGVSTD
                     FPTALRRAEGRVPAGFSLAYQPVVRLPDASPVAIEALARWTAPNGTDIQPDAFVAAAE
                     AAGLGAALDAMVLDLACNEFASSGVDHVLHVNIGAARLGCLEFEQRVLRTLDRCGLTP
                     DQLVLEITETVPVVDLASAAAQITRLKALGIKVALDDYGAGYSSLTYLHALPVQIIKL
                     DRGLAVGPEPDRIETLYRSVIRL"
     gene            complement(2196..3041)
                     /locus_tag="VGP27_15655"
     CDS             complement(2196..3041)
                     /locus_tag="VGP27_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7852767.1"
                     /translation="MIGIALALASALGFGVSDFVGGIASRRVAALRVVLVSYPVALVL
                     LLVLALIVGGDVSHGAMFWGGLCGVSQAFGVWWFYAALGAGPISVVSPLTAILVAAIP
                     VGVGLASGERPGAIAGVGVVLALAAVVLVSRDAADEDITPHKFTTKVAWLTVGSGIAF
                     GLNFVLIAQAPPEAMLWPLVFARLAASVLVLAIAGVTANLRAPTGTPLRLAFLAAVLD
                     AAANVAMLFALQASLLSLAGVLMSLYPAATVLLAIVVLRERVTRPQAVGMAVALISVA
                     MIASA"
     gene            complement(3038..3532)
                     /gene="smpB"
                     /locus_tag="VGP27_15660"
     CDS             complement(3038..3532)
                     /gene="smpB"
                     /locus_tag="VGP27_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV7852768.1"
                     /translation="MAKKADPTRSSNNRVVATNRKARHNYSILETFEAGVALMGTEVK
                     SLRDGAASLADAFATVDDGEIWLRNLHIPEYHHGSWTNHAPRRNRKLLLHRKQIDTLI
                     GKIRDGNLTLVPLSLYFSDGKVKVELALARGKQAHDKRQDLARRDAQREVTRELGRRA
                     KGMT"
     gene            complement(3535..>4214)
                     /locus_tag="VGP27_15665"
     CDS             complement(3535..>4214)
                     /locus_tag="VGP27_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEV7852769.1"
                     /translation="CDADPCKSLRQRIEDRDDVRSVRFLNRDDAYNDAIKKFPQYKDV
                     AGKDAFPASFVVKLDNPEQHKDFDDAIAGQPGVLNVLNQKDLIDRLFAVLDGLSNAAF
                     AVALVQAVGAVLLIANMVQVAAYTRRTEVGIMRLVGASRWYTQLPFLLEACLAALIGV
                     VISIVGLIIVRAFFLDNALSQFYEANLIARVDYADILYIAPILIFVGVAMAGATGYAT
                     LRLYVRR"
BASE COUNT          818 a         1436 c         1324 g          636 t
ORIGIN      
        1 acagccggat gaccgagcgg tacagcgtct cgatccgatc cggctcgggg ccgaccgcca
       61 gaccgcggtc gagcttgatg atctgcacgg gcaacgcgtg cagatacgtc agcgagctgt
      121 agcccgcgcc gtaatcgtcc aaggcgacct tgatgccgag ggccttgagc cgggtaatct
      181 gcgcggccgc cgaggcgaga tcgacgaccg gcaccgtctc ggtgatttcc aggacgagct
      241 ggtccggggt caatccgcag cgatccagcg ttcgaaggac ccgctgctcg aactccaggc
      301 agccgagccg cgccgcgccg atgttgacgt gcaacacgtg gtcgactccg gaggacgcga
      361 actcgttgca cgcaaggtcg agcaccatgg cgtcgagcgc tgcgcccaga ccggcggctt
      421 cggccgcggc gacgaacgcg tcgggttgga tgtcggttcc gttgggggcg gtccaccgtg
      481 ccaacgcctc gatcgcgacc ggcgacgcgt ccggcagtcg caccaccggc tgatacgcga
      541 ggctgaagcc agccggcacg cggccctcgg cccgccggag cgcggtcggg aaatccgtcg
      601 atacccccag cgacgggcgg tagatgaccg cggtgtcctt acccacccgc ttacccgcgt
      661 acatcgagac gtccgcctgc ctcagcagat cgtcggaggt gggcggggga tcttcggcgt
      721 ccggacggac cagccccatg ctcgcgcgca ccctgatcga cgagccgtgg atcgcgaacg
      781 gatcgcgaat ggccaatcgg atgcggtccg cgaccacctc gggtgcttcc cgctccccgc
      841 tgatcaagat cgcgaactcg tcgcccccga tccgtgccaa tgtgtcggcg tcgccgatac
      901 atcgctgcag tctctcaccc accgcacaca gcagttcgtc gccgccggca tggccgaacc
      961 ggtcgttgac ctctttgaag tcgtcgaggt cgacgaagat caacacgaac gcgccggcac
     1021 cgatggcttc gtccagccgc tttgagaaca gcacgcggtt gggcaacccg gtcagtgcgt
     1081 cgtggtgcac ctggtgggcg agccggtgtt gggtctcata gagccggtgc atcaagcgcc
     1141 gctgtgcgcg cattgcaatc acctgtcggg cggtaaccag agtgatgatc accattgccg
     1201 cgtaaagctc gatcacattc agacgacggc cgagggcgag atggaagaca tacagcgacg
     1261 cgatgccgag aaacccgatg tagggcagcg ccagctggac ccaatccaag gcacttcgtc
     1321 cgcgacccgg cgccggccgc ggcgcgaagt ccagcaccga ataagcgatc agcaactggc
     1381 ctaataggaa tccgatgctt ccccagaggt ggccgccttc atggccgacg gttcggaggt
     1441 agatcaccat ccgatccgag gcggcgatgg tgagcgcacc gcacgcgaga agaaggaaat
     1501 tggcccggta caagcggttc gcaccatagg cgattgcagt gagaaccgcg gtgaccacca
     1561 tcacgagttc gatcaccgcg taggcgacaa gcaccaatgt gttctccgac cggggcagca
     1621 cggccccggt gctgtcgccg aacccggaca tcagcaccag catcccgaaa gaaatggccg
     1681 ccaccaggcc gtcgaggacc gtgatgatgc gcgaagcccg gatgggatcc tctggttctt
     1741 cggtgaccgg gccaccggcg cgcaccagca ccaccgcgat caccaaagtc agcaacggcg
     1801 gcatgaagta cagggcgacg gcagggggcg gtgcagtgcc gtcgtcggtg acacggagcg
     1861 cataccagag gaggtcaccg gcccctgagg acaggaaccc agcgaccacg acgagccgcc
     1921 agcccctcgt caagccgcgc ttgcgccgtg cgaccaccag cgcgcacgtg attgcgccga
     1981 ggccgatgat cgcctgcagt gccgtgaaca cccatcgggc agtctggtcg ccccagagtg
     2041 cgcacgcgtt gacgcataac agcgccaaca gggcggcaaa gaggccgagg cgcagctgac
     2101 cgcctctgat cgacacccga cccctacctc tcatgttccc tggcaaacac tcaactgcca
     2161 tgatgcctta tccgagcaaa ccccagtggc ggcaatcagg ccgaggcgat catggcgacc
     2221 gatatcagcg ccaccgccat gccgaccgcc tgcgggcggg taacccgctc gcgcagcacg
     2281 acgatcgcca gcaacaccgt cgcggccgga tacagcgaca tcagcacacc ggccagcgac
     2341 agcagcgacg cctgcagcgc gaacagcatc gccacattgg ccgcggcgtc caatactgcg
     2401 gccaggaagg ccagccgtag tggggttccg gtcggcgctc gcaggttggc ggtcaccccc
     2461 gcgatggcca gtaccaagac gctggccgcc aaccgcgcga acaccagcgg ccagagcatg
     2521 gcctcgggcg gcgcctgcgc gatcagcacg aagttcagcc cgaacgcgat tcccgagccc
     2581 accgtcaacc acgcgacctt tgtcgtgaac ttgtgcgggg tgatgtcctc gtcggcagca
     2641 tctcggctga ccaggaccac ggccgccagc gcgagcacga cgccgacgcc tgcgatggcg
     2701 cccggccgct cccccgacgc gagtcctacg ccgacgggta tcgccgcgac cagaatcgcc
     2761 gtcagcggcg acaccaccga aatcggcccc gcgcccaacg cggcgtagaa ccaccagacg
     2821 ccgaacgcct gcgacacacc gcacaggcca ccccagaaca tcgcgccgtg cgagacgtcg
     2881 ccgccgacga tcagcgccag caccaggagc agcaccaagg ccaccgggta ggacaccagc
     2941 accacccgca acgctgccac ccgtcgcgat gcgattccgc cgacgaaatc gctgacgccg
     3001 aatcccagcg ccgacgccag cgcgagcgcg atcccgatca ggtcatgccc ttggcgcgtc
     3061 gacccagttc ccgggtcact tcgcgctgcg cgtcgcggcg ggcgaggtcc tggcgcttgt
     3121 cgtgggcctg cttgccgcgg gccagcgcga gttcgacctt gaccttgccg tcggagaagt
     3181 acagcgacag cggcaccagg gtcaggttgc cgtcgcggat cttgccgatc agcgtgtcaa
     3241 tctgtttgcg gtgcaacaac agcttgcgat tgcgccgcgg tgcgtggttg gtccagctgc
     3301 cgtggtggta ttcggggatg tgcaggttgc gcaaccaaat ctcgccgtcg tcgacggtgg
     3361 cgaacgcgtc ggccaacgag gccgcgccgt cgcgcaggct cttcacctct gtgcccatca
     3421 gcgcaacacc cgcctcgaac gtctcgagga tcgaatagtt gtgtcgcgcc ttgcgattgg
     3481 tcgcgaccac tcggttgttg cttgacctcg tcgggtcggc tttcttggcc acgatcaacg
     3541 tctgacgtac aggcgcaggg tggcgtagcc ggtggcaccg gccatcgcga cgccgacgaa
     3601 gatcaatatc ggcgcgatgt agaggatgtc ggcatagtcc actctcgcaa tgagattggc
     3661 ttcgtagaac tgactcagcg cgttatccag gaaaaacgcg cgcacgatga tcaagccgac
     3721 gatcgagatg accacaccga tgagggcggc cagacaggct tccaacaaga agggcagctg
     3781 cgtataccag cggctcgcac cgaccagacg catgatgccc acttcggttc gacgggtgta
     3841 agccgccact tgaaccatgt tggctatcaa cagaaccgct ccaaccgcct gcacgagcgc
     3901 caccgcgaac gctgcgttgg aaagcccgtc aagaaccgcg aacagccggt cgatcaaatc
     3961 cttctggttc agtacgttca acacacccgg ctggccggcg atcgcatcgt cgaagtcttt
     4021 gtgctgctct ggattgtcga gcttgacgac gaacgacgcg ggaaacgcat ccttgcccgc
     4081 gacgtccttg tactgcggga acttcttgat cgcatcgttg taggcgtcgt cgcggttcag
     4141 aaaacgtacg gaccgcacgt catctctatc ttcgattctc tgccgcagcg acttgcacgg
     4201 gtcggcgtca cacg
//
LOCUS       DASYLX010000661         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_803176,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000661 DASYLX010000000
VERSION     DASYLX010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803176"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..851
                     /locus_tag="VGP27_15670"
     CDS             18..851
                     /locus_tag="VGP27_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEV7852770.1"
                     /translation="MEAPELVALMQGRRVAVLTGAGMSTDSGIPDYRGPDSPPSNPMT
                     IRQFTSDPLFRQRYWARNHVGWRHMDDTLPNAGHRALAALEHAGVVSGVITQNVDLLH
                     TKAGSRNVVNLHGTYAEVVCLNCGQTMSRAALAEMLEAANPGFVERAEAVGGIAVAPD
                     ADAIIGDTSSFTIIDCTNCGGMLKPNIVYFGESVPKGRVAQAFTMVDEADALLVAGSS
                     LTVFSGFRFVRHAASHGIPVAIINRGPTRGDDLAAVKIDVGCSPMLALLADELPIMTS
                     A"
     gene            848..1477
                     /locus_tag="VGP27_15675"
     CDS             848..1477
                     /locus_tag="VGP27_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852771.1"
                     /translation="MNIPPLSLLNRLRPKSCPAEERWSLSIGDVVADTVSAPEPIRRV
                     VRLLNHFGGVALSGQSVEFDGDDVEWSDVTDIRTRNLVGYLLSDAIDKQVGKLPLPRF
                     PGRGLLLDALGATVLTALVAVAGQQLENGPLDVRIPAEVHYRGMLRGKEISPGVLAAL
                     VLSDPAVRECLQVTARSHGVKVRPSDDDAVEAAAHRADRIKSAISKLTS"
     gene            complement(1480..>1828)
                     /locus_tag="VGP27_15680"
     CDS             complement(1480..>1828)
                     /locus_tag="VGP27_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEV7852772.1"
                     /translation="SVGTVFAPRPERMSARRFWVRYAAESAGTLTLDDGAVRAVVKQR
                     RSLLPAGITAVAGRFHGGDVVELRAPDETMVARGVVAYDAAELTTMLGRSTSDLPAEM
                     RRPAVHADDLVAV"
BASE COUNT          297 a          645 c          602 g          284 t
ORIGIN      
        1 cgcctaccct cgatggggtg gaagcccccg aactcgtcgc cctgatgcag ggacggcggg
       61 tagcggtgct caccggcgcg ggtatgtcca ccgactccgg gattccggac taccgcggac
      121 cggactcgcc gccgagcaac ccgatgacga tccggcagtt cacctccgac ccgctgtttc
      181 ggcagcggta ctgggcccgc aaccacgtcg gctggcggca catggacgac accctgccca
      241 acgccggtca ccgcgcgctg gcggcgctcg agcacgccgg tgtggtctcg ggcgtcatca
      301 cccagaacgt cgacctgctg cacacgaagg ccggtagccg caacgtcgtc aacctgcacg
      361 gcacctacgc cgaggtggtc tgcctgaact gtggccaaac gatgagccgc gccgccctgg
      421 cggagatgct cgaggcggcc aatcccggat tcgtcgagcg cgccgaggca gtcggtggca
      481 tcgcggtcgc acccgacgcc gacgcgatca tcggcgacac atcgtcgttc acgatcatcg
      541 actgcaccaa ctgcggtggc atgctcaagc ccaatatcgt ctacttcggc gagagcgtcc
      601 ccaaaggccg tgttgcacag gcgtttacaa tggtcgacga ggccgacgcg ctgctggtgg
      661 ccggttcctc gttgacggtg ttctccggat tccgattcgt gcgtcacgcc gcgtcgcacg
      721 gcattcccgt cgcgatcatc aaccgtggtc ccacccgcgg cgacgacctg gcggccgtga
      781 agatcgacgt cggctgctcc cccatgctcg cgctgctcgc cgacgaactc ccgatcatga
      841 cgtcggcgtg aacataccgc cactgtcgct cttgaaccgg ttgcggccga agtcctgtcc
      901 cgccgaagag cgctggtcgt tgagcatcgg cgacgtcgtc gctgatacgg tcagtgcgcc
      961 ggagccgata cgccgcgtcg ttcggctgct caaccacttc ggcggcgtgg cgctgagcgg
     1021 acagtccgtc gagttcgacg gcgacgacgt ggagtggtcg gacgtcaccg atatccgcac
     1081 ccgcaacctg gtcggctatc tgctgtccga cgcgatcgac aagcaggtcg gcaagctgcc
     1141 gttgccacgc ttccccggcc gcggactgct gctcgacgcg ctgggagcca cagtgctgac
     1201 cgccttggtc gcggtggccg gacagcaact cgaaaacggc ccgctggacg tgcgcatacc
     1261 ggcggaggtg cactaccgcg gcatgctgcg cggcaaggaa atctctccgg gcgtgctggc
     1321 cgcgctagtg ctgtccgacc cggcggtgcg cgaatgcctg caggtgacgg cccgcagcca
     1381 cggcgttaag gtacggccgt ccgatgacga cgcggttgaa gcggcggcac accgcgcgga
     1441 ccgcatcaag tccgcgatct cgaaactgac ctcgtgattt cagacggcga cgaggtcgtc
     1501 ggcatgcacc gcaggacggc gcatctcggc gggtagatca gacgtcgacc ggccgagcat
     1561 cgtcgtcagc tcggcggcat cgtaggccac cacgccgcgg gccaccatgg tctcgtcggg
     1621 cgcgcgcagt tcgacgacat cgccgccgtg gaagcgcccc gctacagccg tgatgcctgc
     1681 gggcagtagt gaccggcgtt gcttcaccac cgcgcggacc gcgccgtcgt cgagcgtcag
     1741 cgtgcccgcc gactccgcgg cataacgcac ccagaagcgg cgcgccgaca tgcgttccgg
     1801 ccggggcgcg aacaccgtgc caaccgac
//
LOCUS       DASYLX010000662         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_583600,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000662 DASYLX010000000
VERSION     DASYLX010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583600"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGP27_15685"
     CDS             complement(<1..602)
                     /locus_tag="VGP27_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7852773.1"
                     /translation="MAATTPRDQRGYHAFVFDMDGVVTDTAKVHAEAWKALFDDVLAR
                     IGRPGQPPFDPVHDYLAYIDGRTREDGIRAFLAARGITLPEGSPDDGPERMTVHGLAE
                     RKQQLFAEAIARTGVTVFPDAADLLAKLLDRRIPTALVTASRNGRGILDATDLSGMFT
                     TIVDGSDVARLGLPGKPDAAMFLEAVDRLGVEPADAVVLE"
     gene            complement(762..1568)
                     /locus_tag="VGP27_15690"
     CDS             complement(762..1568)
                     /locus_tag="VGP27_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7852774.1"
                     /translation="MNNTADDVALALSLADEAESMTMPRFTAVDLYAKARLEDTPLTE
                     AYRDVDVALRRRLAMKRPQDTTFETLRGPKQFAGRIWIIEPLDGRKNFIRGVPVWATL
                     IALMVDGVPTVGVVSAPALHQRWWAAAGQGAYATRWAEAARRLSVSAIDELGAASLSF
                     SSLSSWGVLGLRERFIVLTDDVWRARGYGDFYSYCLVAEGTADIALAAQVSVRQLAAP
                     DILVREAGGTFTDLGGSPGPHGNTAAATNGPLHNAVLGRFYGTAAVHQTM"
     gene            1949..2242
                     /locus_tag="VGP27_15695"
     CDS             1949..2242
                     /locus_tag="VGP27_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV7852775.1"
                     /translation="MVTKALLVRLEAHPGKGDAVEDFLVWARLLVEEEPGTKPWLAVR
                     FDASTFGIIEAFPDDEARRIHLAGPFGQELKEKAELFASPPQIVNLDVLAEKL"
     gene            2437..2661
                     /locus_tag="VGP27_15700"
     CDS             2437..2661
                     /locus_tag="VGP27_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEV7852776.1"
                     /translation="MTRDWGPWLVGGGLLLIVGGLLAWTGGLSWLGNLPGDIRIDSGH
                     TRIFIPITSMVLVSVALNLLLWFFLAIFRQ"
     gene            complement(2680..3060)
                     /locus_tag="VGP27_15705"
     CDS             complement(2680..3060)
                     /locus_tag="VGP27_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852777.1"
                     /translation="MAVKLNDRAYEHARDLIVAGRCVIDGRDAWSEHQPSTRQENEFI
                     EQEGIVEYSRWHLGIDDALDENRKGRYKFPYGDFHDVHRCGLLAAESRAGQRKYFDIE
                     LAVAHLHGMLEVLRRSVTPPSHGS"
     gene            complement(3326..>3568)
                     /locus_tag="VGP27_15710"
     CDS             complement(3326..>3568)
                     /locus_tag="VGP27_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV7852778.1"
                     /translation="AVEEFLLSALPLVEQEPGTKPWLAVRFGPSTFGIIDAFPDEAAR
                     QTHLGGPVGKALAEKADELFASPPDISNLDVIADKL"
BASE COUNT          604 a         1130 c         1156 g          678 t
ORIGIN      
        1 tcttcgagga cgacggcgtc tgccggttcc acaccaaggc ggtcgacggc ctcgaggaac
       61 atggcggcgt cgggcttgcc cggtagcccc agccgcgcga cgtcggaccc gtcgacgatg
      121 gtggtgaaca tgcccgacag atcggtggcg tcgaggatgc cccggccgtt gcggctcgcg
      181 gtgacgagcg cggtgggaat gcggcggtcg aggagtttgg cgagtagatc ggcggcatcg
      241 gggaacaccg tgaccccggt gcgggcgatt gcttcggcaa acagttgctg cttacgttcg
      301 gcgagcccgt ggacggtcat ccgctccggg ccgtcgtcgg ggctgccttc cggcagtgtg
      361 atgccgcgag cggccaggaa ggcacgaatg ccgtcctcgc gggtgcggcc gtcaatgtag
      421 gcgaggtagt cgtgcaccgg atcgaacggc ggttgacccg gtcttccgat gcgggccagc
      481 acgtcgtcga acagggcttt ccacgcctcg gcgtggacct tcgcggtgtc ggtgaccacg
      541 ccgtccatgt cgaagacgaa cgcgtggtag ccccgttggt ctcgtggcgt cgtcgccgcc
      601 atatgtgtcg agtgtattga cggcatgccc gggcgacttc gacccgccag ggcaaaaaac
      661 gccgtctcgg atgcgtctga tctgccgggg tgattctcgg cgctccagac gaaacggcga
      721 tgactgcgca tgtccggccg acgccgacac cgccgccgtg ctcacatcgt ctgatgcacc
      781 gctgcggtgc cgtagaagcg gccaaggacc gcattgtgta gtgggccatt ggttgctgcg
      841 gcggtattgc cgtgcggtcc gggggaaccc cccaggtctg tgaatgtgcc gccggcctca
      901 cgcaccaaga tatcgggggc cgccagctgc cggacagaca cttgcgctgc caacgcgatg
      961 tcggccgtcc cctcggccac caggcagtac gagtagaagt cgccgtaacc gcgcgcccgc
     1021 cagacgtcgt cggtcaggac gatgaagcgc tcacgcagcc cgagaacccc ccagctggac
     1081 aggctggaga aagacaggct ggctgccccc aattcatcga tcgccgagac ggacagccgt
     1141 cgcgccgcct cagcccaccg cgttgcgtac gcgccttgcc cggcggctgc ccaccaacgc
     1201 tgatgcaggg cgggggcgct gacgaccccc acggttggta cgccgtcgac catcaacgcg
     1261 ataagcgtcg cccaaacagg tacaccgcgg atgaaattct tccggccgtc gagcggctcg
     1321 atgatccaga ttcgtccggc gaactgcttt gggccgcgga gcgtttcgaa agtcgtatct
     1381 tgcggtcgct tcatggccaa tcttctgcgc agggcgacat cgacatcgcg gtaagcttcg
     1441 gtcaacggcg tgtcctccag cctcgccttt gcgtagagat ccacggcggt gaaccgaggc
     1501 atcgtcatcg attcggcctc gtctgccaaa gacagcgcga gagcgacgtc atctgcggtg
     1561 ttattcatgc cagtcctccc ctccgggaaa cggatttgcg ctgctcggat ggcgccatgc
     1621 ccttagaagc gttgcctctc ttcaggacga catggtgcct cgatgagagg ccggatgcct
     1681 ccgcctggcg cggcgaatct cggcggcgaa tcggcctcgc gtgccacctc cattctcttt
     1741 tgtcaaggca gtcggggcgt cgtccgaggg gtctaagaga tcgcatcagg agttgacccg
     1801 ccaagccgaa gaggtcgtcg ccaggtcctg ggacacttac aaatccgtgg gcgttggcat
     1861 cggcgcgcta tggatccatc tggcggaccg ggcaacttga aagtcggcgg ttccatcgga
     1921 tctattcctc ggacagggag gcgccgtcgt ggtaaccaag gcattgttgg tgcggctcga
     1981 agcccacccg ggcaagggag atgccgtcga ggatttcttg gtgtgggcac ggctgctcgt
     2041 cgaggaggaa cccggcacca aaccgtggtt ggctgtgcgg ttcgacgcgt cgacatttgg
     2101 gattatcgag gcgttccccg acgacgaggc gcggcgaatc cacttggcgg ggccgttcgg
     2161 ccaggaactg aaagagaaag ccgaactgtt cgcctcaccg ccccagattg tcaatctcga
     2221 tgtgctcgcc gagaagcttt gatacgcgcg acgatcgacg cgatccgacc tgacgagaac
     2281 cgcctaagcg catggcgcta ctacggccgt gttgtctggc atgtaccggt ggcacagggt
     2341 gagccgttcg aactcgtccg acgaggtcga ggctcggcgg ccacccctgc ggccacaatt
     2401 gacatcgcag agaccaccag gtgtggaggg gcaagcgtga ccagagattg gggcccgtgg
     2461 ctcgtcggcg gaggattact gctgatcgtc ggagggctac tcgcttggac cggcggcctg
     2521 tcctggctcg ggaaccttcc tggcgatata cggatcgaca gtggccacac acggatattc
     2581 attccgataa cgtcgatggt gcttgtctcg gtcgccctga acctactcct gtggttcttc
     2641 ctggcgatct tccgccagtg atcaccggtg tgccaactct cagcttccat gagatggagg
     2701 tgtcaccgac ctccgcagga cttccagcat gccgtgaaga tgagcgaccg caagttcgat
     2761 gtcgaagtac ttgcgctgcc cggctcggga ctcggccgcc agcagcccgc agcggtggac
     2821 gtcgtggaag tcgccgtacg gaaacttgta acggcccttg cggttctcgt ccagcgcgtc
     2881 gtcgattccg agatgccatc tcgagtactc cacgatcccc tcttgttcga tgaactcgtt
     2941 ctcctgccgc gtggacggct ggtgctcgct ccatgcatcc cgcccatcga tcacgcacct
     3001 gcctgccaca atcaagtcgc gtgcgtgctc gtatgccctg tcgttcaatt tgaccgccat
     3061 gcgtacatga tcgctgtcat cgccgctccc accttcggcc tggcgcggcg gaattcgtca
     3121 ctcatgcttc tcccaggagg ctgaagcccg gtggacgacc gaccatcacc tcgtcgatct
     3181 gatcttcatg cctgccaacc cgcgtcgcca cgcggtgggt ccgtcgctac ctccgatgag
     3241 gttgatgtgt agcacccgag ggattcctgg ccgtctcggc accacctgtt ggtcaccctg
     3301 accagtcggg tcaggcgcca cggcgtcaga gcttgtcggc gatcacgtcg agattgctga
     3361 tgtcgggtgg tgaggcgaag agctcgtcgg ctttctccgc gagcgccttt ccaacggggc
     3421 cgccaagatg cgtctggcgt gccgcctcgt ccgggaacgc gtcgatgatg ccgaacgtcg
     3481 acgggccgaa ccgaaccgct aaccatggtt tcgtgccggg ttcttgctcg acgagcggca
     3541 gcgctgaaag aaggaattcc tcgactgc
//
LOCUS       DASYLX010000663         5672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_254470,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000663 DASYLX010000000
VERSION     DASYLX010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254470"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VGP27_15715"
     CDS             complement(<1..1131)
                     /locus_tag="VGP27_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7852779.1"
                     /translation="MRNRTRDVAPRRRWALIFAVVILVGVLTAVAVRYGGVGGNKAAD
                     GPSTGTSGACDLPYVTKQIDDHRGIVDWAYPGNPFDAKKAAGKTIFTIQENSANPFTN
                     AIVAGMKDVADRIGMRVVDYPNQGQRTEWIQGIQQATSQKVDVIVLMGGTIGPVYFSE
                     QAAAADRAGIPIVTVVDRDLTQDPEPDTAARVGQPYAQAARLDADWIIKQTNCHANVL
                     VITTNELIAGDINSKAAQDEFDKYCGEGCTTRFVNIPLTQWATKIGTTTQTEVAANHD
                     LDYVFPLYDAMAQFVVPAIQLGGGITRVKVATFNGTPGILRMIQTGSTVEMIVGENET
                     HLGYAAMDQAMRLMTGVDPIGNGDYGIPLRIFDDSNVDETGVP"
     gene            complement(1151..2119)
                     /locus_tag="VGP27_15720"
     CDS             complement(1151..2119)
                     /locus_tag="VGP27_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852780.1"
                     /translation="MSVGEFVERFALVGAWIALVVVFSALKPDTFATSSNFAAILGSQ
                     AVLVVLTCALLIPLTAGDYDLSITGVLTLSQMMIGILNTRHGISVYICIVLALLMGAL
                     VGLINGAFLVIFGVDSFIVTLGTGTFLGGVVLWISNSETFAGISSQLTGPVVVERFLG
                     VPLAFYYGLALVVALWYFLEYTSLGRQLLFVGRSRSVARLSGLRVGRLRWGALVASGV
                     IAAAAGVLYAGITGAADPVSGAGFLLPAFAAAFLGATSIVPGRFNPWGSFIAVYFLVT
                     GITGLALFGIQSWLQDMFYGGALVLAVTFSQIARRREEHESGVPEN"
     gene            complement(2164..3684)
                     /locus_tag="VGP27_15725"
     CDS             complement(2164..3684)
                     /locus_tag="VGP27_15725"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV7852781.1"
                     /translation="MRALHDVDLTIRRGEVHGLLGENGSGKSTLIKILSGFHAPDRGS
                     ELEINGRPVSVPLHPGQFRSLGMSFVHQDLGLVPSLSVVENLFVGDLTSRRSWRLSWA
                     RERRRAEETFTSFGLTLDPRAKVSDLRPTDRALLAIVRAVEEIRANTAEDGSEYGLLV
                     LDEPTVFLPASDRDDLFAIVREIAATRASVLFVSHDLDEILEITDRVTVLRDGVVRGT
                     VDTARATDEVLVEMIIGRPLQALPIERRQTEVGTAVSITGLTGRYAQDVSLTVHKGEV
                     VGLTGLMGSGFEEIPYYVFGARSPAAGRLDVDGKTYDLRGMSPRRALEAGVALVPADR
                     QHDGSVGSLSLGDNVAVPVLDKYRSRIGLNRSRMRSDARKLLEQFDVRPHDPRLTYQS
                     LSGGNQQKAMLAKWLQMKPSLLFLHEPTQGVDVGARQQIFQLTRKAAADGTAVVCVST
                     DYDQLAAVCDRVVVFGRGRLRRELTGDQVTKERIAEQCYRVAESHSGSHLDAGGDA"
     gene            complement(3741..3884)
                     /locus_tag="VGP27_15730"
     CDS             complement(3741..3884)
                     /locus_tag="VGP27_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852782.1"
                     /translation="MTSSDRAAANGERRPNLFDVQAKDGDGAGVAGVGEYLHTGSVEA
                     AAE"
     gene            3960..>5672
                     /locus_tag="VGP27_15735"
     CDS             3960..>5672
                     /locus_tag="VGP27_15735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7852783.1"
                     /translation="MGRTSQLVNDELAQVLGLSLDAFCIAGFDGYLKFANPAFARILG
                     YTQDALLARPLMDRVHPDDVESVGAVLDGLADGKDTSGFECRLVCADGSVCWFKWNTH
                     ARPDKGVVYGVARDAPDRRGASAELTALHRVATLVAEGVQPHDLFAVVAEEVSRVVDA
                     SAVSIVRYELDGTASECANFSRGEKLFPVGVRWSIEGTNILRLIRDSAAAARIDDYAG
                     LDGDLAAFVRSTGIRSTVGVPIVVAGRLWGTIIGSTTGPDPLPEDTASRLASFTELLA
                     TAIQNAKSREALERLVDEQAALRRVATLVASGVRPDEIFAAVSNEVGRLVGTDSATVI
                     RYDDDGEGISYVGSASKVNAAFPVGAHWKFQKGMASFEVYRTGRSARSGAHLITVDGP
                     IGDIHRRLGIISAVASPIVVEGRLWGAMAVQSQQPLTLDTEERLEKFTELVATALANA
                     DSKSELAASRRRIVEASDEARRRIERDLHDGTQQRLVVLMLQLAALRNAAPTGEALIA
                     ELSRVESELASALEELRELSRGIHPSILSEGGLGPALRALARRSTIPVALDVTTERRL
                     AEPIE"
BASE COUNT          936 a         1887 c         1904 g          945 t
ORIGIN      
        1 cggaacgccc gtctcgtcga cgttcgagtc gtcgaaaatg cgaagcggga tgccgtagtc
       61 gccgttcccg atcgggtcga cgccggtcat caggcgcatc gcctggtcca tcgccgcgta
      121 gccgaggtgg gtctcgttct caccgacgat catctcgacc gtgctccccg tctggatcat
      181 gcgaaggatt cccggtgtgc cgttgaaggt cgcgaccttg accctcgtga tcccgccacc
      241 gagttggatt gcagggacga cgaactgcgc cattgcgtcg tacaacggaa agacgtagtc
      301 gagatcgtgg ttcgccgcga cttccgtctg cgtcgtcgtc ccgatcttcg tagcccactg
      361 ggtgagcggg atgttcacga acctcgtcgt gcaaccctca ccgcagtact tgtcgaactc
      421 atcctgcgcg gccttgctat tgatgtcgcc cgcgatcagt tcgttcgtgg tgatgacgag
      481 cacgttcgcg tggcaatttg tctgcttgat gatccagtcc gcgtcgagcc gggcggcctg
      541 ggcatacggc tggccgacgc gtgcggccgt gtccggctcg gggtcctggg tcagatcacg
      601 gtcgacgacc gtgacgatgg ggatgcccgc gcggtcggca gcggctgcct gttccgagaa
      661 gtagaccggg ccgatcgtgc cgcccatcag gacgatcacg tcgaccttct gggaggttgc
      721 ctgctggatt ccctggatcc actctgtgcg ttggccctgg ttcggatagt ccacgaccct
      781 catcccgatc ctgtcggcca cgtccttcat cccggcgacg atcgcgttcg tgaacgggtt
      841 ggcggagttc tcctggatgg tgaagatcgt tttgccggcc gccttcttcg catcgaaggg
      901 gtttcccggg taggcccaat cgacgatccc cctgtggtcg tcgatctgct tggtcacgta
      961 cgggaggtcg cacgcacccg aggtgcctgt gctcggacca tcggccgcct tgttcccacc
     1021 gactccgccg tagcgcacag cgaccgcggt gagcacgccg acgaggatca ccacagcgaa
     1081 gatcaacgcc cagcgccggc gcggtgccac gtcccgggtc ctgttcctca ctcgccgccc
     1141 ctccgttcgg ctagttctcc ggcacgccgc tctcgtgctc ttcccgccgc cgggcaatct
     1201 gcgagaacgt gaccgccaag acgagcgcgc cgccgtagaa catgtcctgg agccacgact
     1261 gaatcccgaa gagggccagc ccggtgatcc cggtgacgag gaagtagacg gcgatgaacg
     1321 atccccatgg attgaaccgg ccggggacga tgctggtcgc tccgaggaac gccgccgcga
     1381 acgcaggcaa caagaacccc gcgcccgaca ccgggtcggc cgcgccggtg atccctgcgt
     1441 agaggacgcc ggcggcggcc gcaatgacgc ccgaggcgac cagcgctccc caacggaggc
     1501 gtcctacccg cagaccgctg agccgggcaa cgctgcgact tcggccgacg aaaagcagtt
     1561 gccggccgag cgaggtgtac tcgaggaagt accagagggc gacgacgagt gcgaggccat
     1621 agtagaaagc aagtgggacg ccgaggaatc gttcgacgac gaccggcccg gtgagctggg
     1681 aggagatccc ggcgaacgtc tccgagttgc tgatccacag gacgacgccg ccgaggaacg
     1741 tccccgtgcc gagcgtgacg atgaacgagt cgacgccgaa aatgaccagg aacgcgccgt
     1801 tgatcagacc gacgagcgcg cccatcagga gcgcgagcac gatgcagatg tatacggaga
     1861 tgccgtgcct ggtgttcagg atcccgatca tcatctgcga cagcgtcagc acccccgtga
     1921 tcgacaagtc gtagtcgccc gcggtcagcg ggatcagcag cgcgcacgtg agcacgacga
     1981 ggacagcctg cgaaccgagg atcgcggcga agttggacga cgtggcgaac gtgtccggct
     2041 tgagcgcgct gaagacgacg accagggcga tccacgcgcc gacgagcgcg aatcgctcga
     2101 cgaactcgcc gacgctcatc cctctgcgca gccgttgcgc gagtgaagcg gtcccggcgc
     2161 tcgttacgca tcccctcccg cgtccagatg actaccggag tggctctcgg cgacgcggta
     2221 gcactgctcc gcgatccgct ccttggtgac ctgatcacct gtcaactcgc gtctgaggcg
     2281 tccgcgaccg aagacgacga cccggtcgca gaccgccgcg agctgatcgt aatcggtgct
     2341 gacgcatacc acggccgtgc cgtcggctgc ggccttccgc gtcagctgga agatctgttg
     2401 gcgcgctccg acgtcgacgc cctgtgtcgg ctcgtggagg aacagcagtg acggcttcat
     2461 ctgcagccac ttggcgagca tcgccttctg ctggttgccg ccgctgagcg actggtacgt
     2521 caaccgaggg tcgtgcggcc ggacgtcgaa ctgttccagc agctttcgcg cgtcgcttcg
     2581 catgcggctt cgattcagac cgatgcgcga tcggtacttg tcaagaaccg gaaccgcgac
     2641 gttgtcgcca agggagagcg agccgacgct cccgtcgtgc tggcggtcgg cggggaccag
     2701 cgcgaccccc gcctcgagcg cacggcgcgg gctcatcccg cggaggtcgt aggtctttcc
     2761 gtccacgtcg agccggccgg cggcggggga tctcgcgccg aacacgtagt acgggatctc
     2821 ctcgaacccg ctgcccatca gcccggtcag gcccacgacc tcgcccttat ggaccgtgag
     2881 cgacacatct tgcgcatatc ggcctgtcag gcccgtgatc gatacggccg tgcctacctc
     2941 cgtctgcctg cgttcgatcg gaagcgcctg aagcggtcgg ccgatgatca tctcgacgag
     3001 cacctcgtcg gtcgctcggg cggtgtcgac ggtgccgcga acgaccccgt ctcgcagcac
     3061 cgtgacgcgg tcggtgatct cgaggatctc gtcgaggtcg tgggagacga agaggacgct
     3121 ggcgcgcgtc gcggcgatct cccggacgat ggcgaagagg tcgtcgcggt cgcttgccgg
     3181 caggaaaacc gtcggctcgt cgaggacgag cagcccgtat tcggacccgt cttcagcggt
     3241 gttcgcacgg atctcctcga ccgctctgac gatcgcaagc agtgcgcggt cggtcggtcg
     3301 caggtcagag accttcgcgc gcggatcgag cgtgagcccg aaggacgtga aggtctcctc
     3361 tgccctgcgc cgttcgcgcg cccaggagag ccgccagctg cgccgggagg tcaggtcgcc
     3421 gacgaagagg ttctcgacga ccgacagcga cgggacgagc ccgagatcct ggtgcacgaa
     3481 gctcatgccg agactgcgga actgccccgg gtgtagcggg accgagacgg gccgtccgtt
     3541 gatctcgagt tcgctgcccc ggtcgggtgc gtggaagccg gagaggatct tgatcagggt
     3601 ggacttgccc gaaccgttct ctccgagcag gccgtgcacc tcgccgcgcc gaatcgtcag
     3661 atcgacgtca tgcagtgcgc gcacagcgcc aaacgtcttc gagaggtgcc tgagcgcgac
     3721 gaccggctga tgctcgtgcg tcactcggct gccgcttcca ccgaacccgt gtggaggtac
     3781 tcgccaaccc cggcgacgcc ggcaccgtcg ccgtccttgg cctgaacgtc gaacaggttc
     3841 ggtcggcgtt ccccgttcgc tgcggcacga tccgagctgg tcatcgaccg aggccagccg
     3901 gcccagggcc cgagaccgcg cccttccgca agacgtacgg agcggtcaca ctggggagcg
     3961 tgggccgtac gagccagctc gtcaatgacg agctggctca ggtcctcggc ctgtcgctcg
     4021 atgccttctg catcgcaggg ttcgacgggt acctgaagtt cgccaatcca gcgttcgcgc
     4081 gcatcctcgg gtacacgcag gatgcgctgc tggccaggcc gttgatggac cgtgtccacc
     4141 ccgacgatgt cgagtccgtc ggcgcggtcc tcgacggcct cgcggacggc aaggacacca
     4201 gtgggttcga gtgtcgcctg gtctgcgccg acggctcggt gtgctggttc aagtggaaca
     4261 cgcacgcccg accggacaaa ggtgtggtgt acggggtggc gagggacgcc ccggaccggc
     4321 gcggagcaag tgccgagctg accgcgcttc accgcgtcgc aaccctcgtg gcggaggggg
     4381 tgcagccgca cgacctcttc gccgttgtcg ccgaggaagt ctcccgcgtc gtcgatgctt
     4441 cggccgtgag catcgtgcgg tacgagctcg acggcacggc gagcgagtgt gccaacttca
     4501 gccggggaga gaagctgttc cctgtcggcg tccggtggtc gatcgaaggc acgaacatcc
     4561 ttcgactcat ccgggacagc gccgccgctg cccgcatcga cgactacgca gggctggacg
     4621 gtgacctggc ggcgttcgtc cgaagcaccg gcatccgttc gacggtcgga gttccgatcg
     4681 tcgtcgccgg tcgtctctgg ggaaccatca tcggttccac cacggggccg gatccgttgc
     4741 cggaagacac cgcgtcgcgg ctggccagct tcaccgagct gctggcgacc gcgatccaga
     4801 atgccaagtc gcgcgaggcc ctcgaacgcc tcgtcgacga gcaggcagcg ctccggcgcg
     4861 tggcaacgct ggtggcgagc ggcgtccgac ccgacgagat cttcgcggcc gtcagcaatg
     4921 aggtcgggcg cctggtcggt acggattcgg ccacggtcat caggtacgac gacgacggtg
     4981 aggggatcag ttacgtcggc tccgcgagca aggtcaacgc agccttcccg gtcggagcgc
     5041 actggaagtt tcagaaggga atggcttcct ttgaggtgta tcgcaccgga cgctcagccc
     5101 gcagcggcgc gcacctgatt acagtcgacg ggccaattgg cgacatccac cgtcgactgg
     5161 gcatcatctc cgcggttgcc agcccgatcg tcgtcgaggg ccgtctctgg ggcgctatgg
     5221 ccgtccagtc ccagcagccg ctgacgctgg acaccgagga acgcctcgag aagttcaccg
     5281 agctcgtcgc aaccgctctc gcgaatgcgg acagcaagtc cgagctcgcc gcctcgcgca
     5341 ggcggatcgt cgaggcgtcg gacgaagccc gccgccggat cgagcgagat ctccacgacg
     5401 gcacgcagca acggctggtg gtgctcatgc tccagctggc agctctcagg aatgcggccc
     5461 ccaccgggga ggcactcatc gcggagttgt cgcgcgtcga gagcgagctc gcgtccgcgc
     5521 tcgaggagct gcgcgagctg tcgcgcggta tccatccgtc gatcctgtcc gagggcggtc
     5581 tcggcccagc tttgcgggcg ctcgcgcggc gctccacgat tcccgtggcg ctcgacgtca
     5641 cgacggagag acggctcgct gagccgatcg ag
//
LOCUS       DASYLX010000664         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3544816,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000664 DASYLX010000000
VERSION     DASYLX010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544816"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGP27_15740"
     CDS             <1..459
                     /locus_tag="VGP27_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV7852784.1"
                     /translation="PTAARALAAEYGSLEAIMSASEEELSVVEGVGPTIAAAVTEWFS
                     VDWHRAIIDKWRAAGVRMADERDAGVARTLEGLSIVVTGSLTNFSRDDAKEAIVARGG
                     KAAGSVSKKTAYVVAGDAPGSKYDKAVELGVPVLDEDAFGRLLENGPDGV"
     gene            535..789
                     /locus_tag="VGP27_15745"
     CDS             535..789
                     /locus_tag="VGP27_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852785.1"
                     /translation="MTICATCGNDYDKAFTVTWPDGRSENFDSIECAAVQLAPECAHC
                     GCRILGHGIETDDGIFCCAHCARKDSNADVNDRYPVPAGA"
     gene            complement(794..1453)
                     /locus_tag="VGP27_15750"
     CDS             complement(794..1453)
                     /locus_tag="VGP27_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEV7852786.1"
                     /translation="MPSFMLRVQLEDRPGRLGSLAVALGSVGADILSLDVVERGAGYA
                     IDDLIVDLPPGAMPDMLITAAENLKGVRVDSIRPHTGLLEAHRELELVDHIAAAPNRH
                     AKLQVLAQEAPRVLRVGWCTVVRLTGPNISRVVGSFGAPETHLNEVPWLPIDNAEALD
                     GDADWVPQVWRDMDTTLAAAPLGDQNIAVVLGRPGGPAFRPSEVARLGYLAGIVATIL
                     R"
     gene            1524..1823
                     /gene="gatC"
                     /locus_tag="VGP27_15755"
     CDS             1524..1823
                     /gene="gatC"
                     /locus_tag="VGP27_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV7852787.1"
                     /translation="MSQISRDEVAHLARLARLALSDDELDSFAGQLDAILEHVSRIQA
                     VDVTDVEATDSPLTSVNVTRPDVVEPGLTQEEALAQAPKSVEGRFAVPRILGEPE"
     gene            1820..3319
                     /gene="gatA"
                     /locus_tag="VGP27_15760"
     CDS             1820..3319
                     /gene="gatA"
                     /locus_tag="VGP27_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV7852788.1"
                     /translation="MSTRGESSDVIRLDAATLGAKIAAKELSSAELTRACLDRIAETD
                     DKYRAFLHVAAEQALEAAGRVDASIAAGDELPSPLAGVPLALKDVFTTTDMPTTCGSK
                     ILEGWTSPYDSTVAVRLRAAGIPILGKTNMDEFAMGSSTENSAFGPTRNPWDVERVPG
                     GSGGGSAAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSL
                     DQGGPCARTVLDTALLHQVIAGHDRKDSTSIDAEVPDIVGAARAGADGDLRGVKVGVV
                     RQLRGDGYQQGVLSSFNAAVEKLTELGAEVSEVDCPHFDYSLPAYYLILPSEVSSNLA
                     RFDGMRYGLRVGDDGTHSAEEVMAMTRAAGFGPEVKRRIMIGAYALSAGYYDAYYNQA
                     QKVRTLIARDLDAAYQKVDVLVSPATPTTAFPLGEKVDDPLAMYLFDLCTLPLNLAGH
                     CGMSVPSGLSPDDNLPVGLQIMAPALADDRLYRVGAAYEAARGALPTAV"
     gene            3364..4395
                     /locus_tag="VGP27_15765"
     CDS             3364..4395
                     /locus_tag="VGP27_15765"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEV7852789.1"
                     /translation="MRIGILTGGGDCPGLNAVIRAVVRTCDVRYGSSVVGFLDGWRGL
                     LEDRRVQLRNDGRNDRLLAKGGTMLGTARVHPDKLKAGLDQIKQTLDDNGIDVLIPIG
                     GEGTLTAAHWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALGVASEAIDRLHSTAES
                     HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDVEEVCRLIKARFVRGDAHFI
                     CVVAEGAKPATGSMKLREGGIDEFGHEKFTGVAQQLASEVEKRINKEVRVTVLGHVQR
                     GGTPTPYDRVLATRFGVNAADAAHAGEYGMMVSLRGQEIGRVPLADAVRQLKLVPQSR
                     YDDAAEFFG"
BASE COUNT          722 a         1488 c         1549 g          652 t
ORIGIN      
        1 ccgacggccg cccgcgcgct ggcagccgaa tacggcagcc tggaggcgat catgtcggcc
       61 tccgaggagg agctgtcggt cgtcgagggg gtcggaccga cgatcgccgc ggcggtcacc
      121 gaatggttca gcgtcgactg gcaccgcgcc atcatcgaca agtggcgcgc cgcgggggtg
      181 cggatggccg acgaacgcga cgccggcgtc gcgcgcacgc tggagggatt gtccatcgtc
      241 gtgaccggat ccttgaccaa cttctcgcgt gacgacgcca aggaggccat cgtcgcgcgc
      301 ggcggcaagg cggccgggtc ggtgtcgaag aagacggcgt acgtcgtggc aggcgacgcg
      361 cccggatcga agtacgacaa ggccgtcgag ctcggggtgc cggtcctcga cgaggacgcc
      421 ttcggccggc tgctggaaaa cggtcccgac ggggtctgag gcccagccgc ggtagtttgc
      481 cgctcgcgat ggccgggaat gcgtcggagc gaaccgatgt gagggagtga cggcatgacc
      541 atctgcgcga cgtgcggcaa cgactacgac aaggcgttca cggtgacctg gcccgacggc
      601 cgcagcgaga acttcgacag catcgagtgc gctgccgtcc agctggcgcc ggagtgtgca
      661 cactgcggat gccggatcct gggccacgga atcgagaccg acgacggcat cttctgctgc
      721 gcacactgcg caaggaagga cagcaacgcc gacgtcaacg accgataccc ggtgcctgca
      781 ggcgcctgac ggttcatctc aggatcgtcg cgacgatgcc ggcgagatag cccagccgcg
      841 ccacctccga cggccggaac gccggaccac cgggcctgcc gagcaccacg gcgatgttct
      901 gatcgcccaa tggtgcggcc gccagggtgg tgtccatatc ccgccacacc tgcggcaccc
      961 agtcggcgtc accgtcgagc gcctcggcgt tgtcgatcgg cagccagggc acctcgttga
     1021 ggtgtgtctc tggtgcgccg aaactgccga cgacgcgact gatgttgggc ccggtcagcc
     1081 gcaccaccgt gcaccagccg acgcgcagca cgcgcggcgc ctcttgggcc agcacctgta
     1141 atttcgcgtg cctgttcggc gccgcggcga tgtgatcgac cagctcgagc tcgcggtgcg
     1201 cctcgagcaa cccggtgtgc gggcggatgc tgtccacccg cacacccttg aggttctccg
     1261 cggcggtgat cagcatgtcg ggcatcgcgc cgggcggcag gtcgacgatc aggtcgtcga
     1321 tcgcgtagcc cgcgccgcgc tccaccacat cgagggacag gatgtcggca cccaccgagc
     1381 cgagcgcgac ggccagcgag cccagcctgc ccggtcgatc ctccagctgg acccgcagca
     1441 tgaacgaagg cacgcgcaac actgttgcac aggggtacgg gctcggcatg ccggcctttc
     1501 gcgcatgact acgctttgat gtcgtgtccc agatctcccg agacgaggtt gcccacctcg
     1561 cccgcctggc ccggctcgcc ctgagcgacg acgagctgga cagcttcgcc ggccagctcg
     1621 acgccatcct cgagcacgtc agccggatcc aggccgtcga cgtcaccgat gtcgaagcca
     1681 ccgacagccc gctcacgtcg gtaaacgtca cccggcccga cgtcgtcgag ccggggctga
     1741 cgcaggagga ggcgctggcg caggcaccga agtcggtgga gggccggttc gccgtgccac
     1801 ggattctggg ggagcccgaa tgagcacccg aggcgagagc agcgacgtga tcaggctgga
     1861 cgcggcgacg ctgggcgcca agatcgccgc caaggagctg tcttcggccg agctcacccg
     1921 cgcctgcctg gaccggatcg ccgagaccga cgacaagtac cgggcgttcc tgcacgtcgc
     1981 agccgagcag gcgctggagg cggccggccg ggtggatgcc tccatcgcgg caggcgatga
     2041 gctgccgtcg ccgctggccg gtgtcccgtt ggcgctcaag gacgtcttca ccacgacgga
     2101 catgccgaca acatgcgggt cgaaaatcct cgagggctgg acatcgccct atgactcgac
     2161 ggtcgcggtg cggctgcgcg ccgcgggcat cccgatcctc ggcaagacca acatggacga
     2221 gttcgcgatg ggttcgtcaa cggagaactc ggccttcggc cctacccgca atccatggga
     2281 cgtcgagcgg gtgccgggcg gctccggcgg cggcagtgcg gcggcgctgg ccgcgttcca
     2341 ggcgccgctg gccatcgggt cggacaccgg cggctcgatc cggcagcccg ccgcgttgac
     2401 cgcgaccgtc ggcgtcaagc ccacctacgg cacggtgtcg cggtacggcc tcgtcgcctg
     2461 cgcgtcgtcg ctggatcagg gcggaccttg tgcgcgcacg gtgctcgaca ccgcgctgct
     2521 gcaccaggtg atcgcgggcc acgaccggaa ggactcgacg tcgatcgacg ccgaggtgcc
     2581 cgacatcgtc ggtgcggcca gggcgggcgc cgacggcgat ctgcgcggtg tcaaggtcgg
     2641 tgtggtccgt cagttgcgcg gcgacggcta ccagcagggc gtgctgtcgt cgttcaatgc
     2701 cgcggtcgag aagctgacgg aactcggcgc cgaggtgtcc gaagtcgact gcccgcactt
     2761 cgactactcg ctgcccgcct actacctgat cctgccgtcg gaggtgtcgt ccaacctggc
     2821 ccggttcgac ggtatgcggt acggccttcg ggtcggcgac gacgggacac acagcgccga
     2881 ggaagtcatg gcgatgacgc gcgccgccgg cttcgggccg gaagtcaagc gccgcatcat
     2941 gattggcgcc tatgcgttgt cggcgggcta ttacgacgcg tactacaacc aggcgcagaa
     3001 ggtgcgcacg ctgatcgccc gcgaccttga tgcggcctac cagaaggtcg atgtgctggt
     3061 gtcgcccgcg accccgacca ccgcgttccc gctgggggag aaggtcgacg atccgctggc
     3121 gatgtacctg ttcgatctct gcacgctgcc gctgaacctg gccgggcact gcggcatgtc
     3181 ggtgccgtcg ggtctatcgc ccgacgacaa cctgcccgtc ggacttcaga tcatggctcc
     3241 ggcgctggcc gacgaccggc tctaccgcgt cggtgcagcc tacgaggcgg cccgcggagc
     3301 actgccgacg gcggtgtagc ccgacagggt gcccacaacg gcgacacagg ggaggatggg
     3361 cacatgcgca tcggaattct gaccggaggc ggcgactgtc cagggctgaa cgcggtgata
     3421 cgcgcggtgg tgcgcacctg cgatgtgcgc tacggctcat cggtcgtggg ctttctggac
     3481 ggctggcgcg gtctgctcga ggaccgtcgc gtccagctgc gcaacgacgg tcgcaacgac
     3541 cgcctgctcg ccaagggcgg caccatgctg ggcaccgcgc gggtgcaccc ggacaagctc
     3601 aaagccggac ttgaccagat caagcagacg ctggacgaca acggcatcga cgtgctgatc
     3661 ccgatcggcg gcgaaggcac cctgaccgcg gcgcattggc tgtccgagga gaacgttccc
     3721 gtcgtcggtg tgcccaagac gatcgacaac gacatcgact gcaccgacgt cacgttcggc
     3781 cacgacaccg cgttgggcgt cgccagcgag gccatcgacc gcctgcacag cacggccgag
     3841 tcacaccagc gggtgatgct cgtcgaggtg atggggcggc acgcaggctg gatcgcactg
     3901 aatgccggcc tggcttccgg cgcgcacatg acgctgattc ccgagcagcc gttcgacgtc
     3961 gaggaggtgt gccggctgat caaggcgcgc ttcgtgcgcg gcgatgcgca cttcatctgc
     4021 gtggtggccg aaggcgccaa gcccgcgacc ggctcgatga agttgcgcga gggcggtatt
     4081 gacgagttcg gacacgagaa gttcaccggc gtcgcccagc agctggcctc cgaggtggag
     4141 aagcggatca acaaagaggt gcgggtcacc gttctgggcc atgttcagcg cggcggcacg
     4201 ccgaccccgt acgaccgagt gctcgccact cgattcggcg tcaacgccgc cgacgccgcc
     4261 cacgctggcg agtacgggat gatggtgtcg ctgcgcggcc aggagatcgg ccgcgtgcca
     4321 cttgccgacg ccgtccgtca gctgaagctg gtgccccaga gccgatacga cgacgccgcc
     4381 gaattcttcg gctgatttct tcggcgagca g
//
LOCUS       DASYLX010000665         6051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_473481,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000665 DASYLX010000000
VERSION     DASYLX010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473481"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VGP27_15770"
     CDS             complement(<1..97)
                     /locus_tag="VGP27_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein pafC"
                     /protein_id="HEV7852790.1"
                     /translation="MSPVSERLVRLLNMVPYFQANPRITHAEAASD"
     gene            complement(94..1086)
                     /locus_tag="VGP27_15775"
     CDS             complement(94..1086)
                     /locus_tag="VGP27_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV7852791.1"
                     /translation="MGISKVERLMNLVIALLSTRTYISAERIRETVYGYSDSASDEAF
                     SRMFERDKNELRDLGIPLETGRVSQFDPTEGYRINRDAYALPAVDLTADEAAAVAVAT
                     QLWESPELITATQGALLKLRAAGVDVDAGDAGIAITPTAGMPGIRGSEEVLGILLSAI
                     NSGQAVQFPHRPARSEPYTTRTVEPWGVVTDRGRWYLVGHDRDRNATRTFRLSRIGAD
                     VKAIGPAGAVTPPDRVNLREVVHKVIGETPTGTQARVWVADGRATALRRLALVVGPRE
                     LDGRAGEEISIDIGMSDRLAREIASYGADAVVLEPQALRDEVLERLRAQAEVGV"
     gene            complement(1120..2496)
                     /locus_tag="VGP27_15780"
     CDS             complement(1120..2496)
                     /locus_tag="VGP27_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852792.1"
                     /translation="MSSCDWDLFCADVQGRVIAGAADQFARSTWNAERIAAAQCEGLQ
                     TLLRHAFERSPFHRRRLAGIDIEDIAPADLSALPLMTKTEMMDGLDDVFTDRRLTCGT
                     VESALAATGIDPVPILDDYIALASGGCSGRRGIFVLDRAAVADFVLSAARQPGPTQPF
                     SAAPGEQPSMAFVASPSAVHATGMLAALTRGGMTPVWSHLVPATLPLSEIVERLNGLR
                     PTVLAGYASMLSGLGAEASAGRLRIAPVTVSSTSETLLPEMRAAIRTAFGVPVVDGFG
                     STEGLVGKTCPDDDVFVFNSDLCIVELVDACNNPVAPGAPSAKVLVTNLYNVTQPLIR
                     YELTDTFIRQPDAADHGYLRARVQGRNDDVLHYGTVDVHPIVIRSVMVKTPEVIDYQV
                     RQTRCGVDVFAVTADAFRLEDLSDRLCAALVDGGLRSPDVTVRPVDFLDRHPVTGKLR
                     RFIPLTTA"
     gene            complement(2496..3095)
                     /locus_tag="VGP27_15785"
     CDS             complement(2496..3095)
                     /locus_tag="VGP27_15785"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852793.1"
                     /translation="MARLRRADQVARNRDLVLAAARRVFLERGYVGASVDAIAEKAGF
                     SVGVVYSQFGSKADLFFALLERRIEDRAAENDRIADEFSGADGVRALLRAGQHDAAAE
                     PGWPYLLAEFRAIAMRDADLSERYARSHSRTLDGIASAFEKLYEPIGLKPPVPIRSIA
                     EFVQAGAVGTALERAANPDALPEEDAERLLLRALGLEET"
     gene            3482..4072
                     /locus_tag="VGP27_15790"
     CDS             3482..4072
                     /locus_tag="VGP27_15790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852794.1"
                     /translation="MGTRWQDTSLPRGDDYDDRWRKLAAAGGSIHGEADLVESLSRNA
                     GATRVLDAGCGTGRVAIELAHRGFSVVGIDSDPAMLHTARSKAPELSWIHADLAEMDG
                     HLDTEFDVVVAAGNVMIFLAPWSERRVVDQLATRLAPDGLLVCGFKLNTGRLTLDRYD
                     EYTTAAGLELVHRWSTWDREPFFGDDYAVSAHVRSK"
     gene            complement(4146..5504)
                     /gene="pafA"
                     /locus_tag="VGP27_15795"
     CDS             complement(4146..5504)
                     /gene="pafA"
                     /locus_tag="VGP27_15795"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEV7852795.1"
                     /translation="MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDRAGERVLEDLLIDAEQRLADEGI
                     GGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
                     PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSET
                     TTMLKVGTAALVLEMIESGVAFRDFSLDNPIRAIREVSHDLTGRRPVRLAGGRQASAL
                     DIQREYHSRAVEHLQTREPNPQIEQVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLF
                     QRYQDRYNMELSDPKISQLDLAYHDIKRGRGVFDLLQRKGLATRLTTDEEIEDAVDTP
                     PQTTRAKLRGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVKRLIA
                     SM"
     gene            5665..5943
                     /locus_tag="VGP27_15800"
     CDS             5665..5943
                     /locus_tag="VGP27_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852796.1"
                     /translation="MGGNTEVDLNALRAVAEHVTEAADGIAELHWPAVAADELKGSAV
                     GNVPAPDLIAAKLADVIANMRGWAVAARMAADAFERADRGSAERFPRR"
BASE COUNT         1010 a         1989 c         2028 g         1024 t
ORIGIN      
        1 gatccgatgc ggcctcggcg tgggtgatcc tcgggttggc ctggaagtac ggcaccatgt
       61 tcagcagccg gaccaggcgc tcggagacgg ggctcatacg cccacctccg cctgggcgcg
      121 cagccgctcc agcacctcgt cgcgaagcgc ctgcggctcg agcaccacgg cgtcggcgcc
      181 gtagctggcg atctcgcgcg ccagtcggtc ggacattccg atgtcgatgc tgatctcctc
      241 gccggcgcgg ccgtcgagct cccgtggacc gaccaccagt gcgagccggc gcaacgcggt
      301 ggcgcggcca tcggcgaccc acaccctggc ctgcgttccg gtcggcgtct cgccgatgac
      361 cttgtgcacg acctcgcgca ggttgaccct gtcgggcggc gtgaccgcac cggctggacc
      421 gatggccttc acatccgccc cgatccggga cagtcggaag gttcgggtgg cgttgcggtc
      481 acggtcgtgt ccgacgaggt accagcgtcc gcgatcggtg accactcccc agggttcgac
      541 cgtgcgcgtc gtgtagggct cactgcgcgc gggccggtgc gggaactgca cggcctgacc
      601 ggaattgatg gcggacaaca aaattcccag cacttcttcg gatccgcgga taccgggcat
      661 tcccgccgtc ggggtgatgg cgatgcccgc gtctccggcg tcgacgtcca cgcctgcggc
      721 cctcagcttg agcagtgcac cctgcgtggc ggtgatcagc tccggcgact cccacagctg
      781 ggtggccacg gcgacggcgg ccgcttcgtc ggcagtcaaa tcgactgcgg gcagcgcgta
      841 cgcatcgcgg ttgatgcggt aaccctcggt ggggtcgaac tgcgagacgc ggcctgtttc
      901 cagagggatg cccaggtcgc gcagttcgtt cttgtcgcgt tcgaacatcc gcgagaacgc
      961 ctcgtcgctg gcgctgtccg agtagccgta gacggtctcc cggatccgct ctgcactgat
     1021 gtaagtgcgg gtggacagca gcgcgatgac gagattcatc agccgctcga ctttggagat
     1081 acccacaggg tcacagctta gggcgtgccg ggctggccgc tatgcagtgg tgagcgggat
     1141 gaaccggcgt agtttgccgg tgaccgggtg tcggtcgagg aagtcgaccg gccgcacggt
     1201 gacatcgggg cttcgcagcc cgccgtcgac gagtgccgcg cacagccgat cggacaggtc
     1261 ctcgagacgg aacgcgtcgg ccgtcaccgc gaacacatcg acaccgcatc gagtctggcg
     1321 cacttgataa tcgatcacct cgggcgtctt caccatcacg gatctgatca caatgggatg
     1381 gacatcgacg gtgccgtagt ggagcacgtc gtcgtttcgc ccttggacgc gcgcacggag
     1441 atagccgtgg tcggcggcat caggctgccg gatgaacgtg tcggtcagct cgtaacggat
     1501 cagcggctgc gtcacgttgt acaggttggt gacaagcact ttcgccgacg gtgcacccgg
     1561 ggccacgggg ttgttgcagg cgtcgaccag ctccacgatg cacaggtcac tgttgaacac
     1621 gaacacgtcg tcatcgggac acgttttgcc gacaaggcct tcggtggagc cgaagccgtc
     1681 caccacaggt acaccgaatg cggttcggat tgcagcccgc atctctggca gcagcgtctc
     1741 gctggtcgag ctcaccgtca cgggagcgat ccgaagtcgg ccggccgagg cttccgcgcc
     1801 aagcccagaa agcatcgagg cgtatcccgc cagaaccgtg ggccgcagtc cattgagccg
     1861 ttccacgatc tcgctgagtg gcagcgtcgc aggcaccaga tgagaccaca caggtgtcat
     1921 tccaccgcgt gtcagagcgg caagcatgcc ggtggcatgc acagccgacg gcgacgcgac
     1981 gaatgccatc gacggctgct cacccggcgc agcggagaat ggttgggtcg gccccggctg
     2041 gcgggccgcc gacaagacga aatcggcaac cgcggcccgg tcgaggacga atattccgcg
     2101 ccggccggag cagccacccg aggccagcgc gatgtagtcg tccagtatcg ggaccggatc
     2161 gataccggtg gccgccagcg cggactcgac tgtgccgcaa gtgagccggc ggtcggtgaa
     2221 cacgtcgtcg aggccgtcca tcatctcggt cttcgtcatc agcggcagcg cagacagatc
     2281 ggccggggcg atgtcctcga tgtcgatgcc cgccaatctc ctgcgatgga acggagagcg
     2341 ctcgaacgca tgccgtagca gcgtctggag cccctcgcac tgtgcagcgg cgattcgttc
     2401 ggcgttccat gtcgaccggg cgaactgatc cgccgcaccg gcgatgactc gcccctggac
     2461 gtcggcgcag aacaggtccc agtcgcagga actcatcacg tctcctccag tccgagcgcc
     2521 cgcaacaaaa gccgctcggc gtcctcctcg ggaagcgcgt ccgggttcgc cgcacgttcc
     2581 aaggcagtac caacggcacc cgcctgcacg aactcggcga tcgagcgaat gggtaccggc
     2641 ggcttgagac cgattggctc gtaaagcttt tcgaaggccg atgcaattcc gtccagtgtt
     2701 cgcgaatgcg agcgggcata tcgttcactg agatcggcat cccgcatcgc gatcgcacgg
     2761 aactcagcca atagatacgg ccagccgggt tctgccgccg cgtcatgctg ccctgcgcgc
     2821 agcaatgcgc ggacaccgtc ggcgcccgag aattcgtcgg cgatccgatc gttctccgcg
     2881 gctcgatcct cgatgcggcg ctctagcagc gcgaagaaca gatccgcctt gctgccgaac
     2941 tgcgaataga ccacgccgac agagaagccg gccttctcgg cgatcgcgtc cacacttgcg
     3001 cccacgtaac cgcgctccag gaacacccgc cgcgcagcag cgaggacgag gtcgcggttg
     3061 cgtgcgacct ggtcagccct gcgtagcctc gccacggctc caattagata acgtcattat
     3121 ccgaatgtca acgctatctg aatgccgcac agcgccgcca cagacacatg aaggcgatcg
     3181 gcgggcactg ccgcgcggtc aacgcctccg gcggcggggg cggggtggcg ccatcccaca
     3241 cgccctgggt cgatgttgcc tgcgcccccc gtttacgtac caccaggcgc tccggtgtgg
     3301 tttccatatc gattcccgaa gtgatgctct gtccggggca ccatgggtac ggtccgcctg
     3361 cgttggccgt accggcgccg aatcccagag cggcagcggc caggccgccg gttgctcctc
     3421 ttgcgcccag cacagcttat tagcactgct atcgatccca acagtccagt tagggtgagg
     3481 tgtgggcacc cgttggcagg acacctcgct gccccgcggc gatgactacg acgaccgctg
     3541 gcgcaagctg gccgccgcgg gcggaagcat ccacggcgag gccgatctgg tcgaaagtct
     3601 ctcgcggaat gccggcgcca cacgggtact ggacgcgggg tgcgggaccg gtcgggtggc
     3661 gatcgaactg gcacaccgcg ggttctcggt ggtcgggatc gattccgatc cggcgatgct
     3721 gcacaccgct cgcagcaagg cgcccgaatt gagttggata cacgccgatc tcgccgagat
     3781 ggatggccat ctcgacacgg agttcgacgt cgtggtggcg gcgggcaacg tgatgatctt
     3841 tctcgcgccc tggtcggaac gccgtgtcgt cgaccaactc gcgactcgcc tggcacctga
     3901 cggactgctg gtttgcggat tcaagctcaa cacggggcgg ttgacgctgg accgctacga
     3961 cgaatacacg acggcggctg gccttgaact ggtgcaccgc tggtccacat gggaccgcga
     4021 accgttcttc ggtgacgact acgcggtgtc cgcgcacgtg aggagcaaat agactccgcg
     4081 cacgtgagga gcgaatagac tccgcgcacg tgaggagcga atagactccc cgcaacgccg
     4141 cctgcctaca tgctcgcgat gagccgcttg acccgctcgt cgaccgagcg gaacgggtcc
     4201 ttgcacagca cggtgcgctg agcctggtcg ttgagcttga ggtgcaccca gtcgacggtg
     4261 aagtcgcggc cggcttcctg tgcggcgctg atgaactcac cgcgcaattt ggcccgtgtg
     4321 gtctgcggcg gagtgtccac cgcatcctcg atctcctcgt cggtggtgag ccgggtggcc
     4381 agacccttgc gctgcagcag gtcgaagacg cccctgcccc gcttgatgtc gtggtaggcc
     4441 aggtcgagct ggctgatctt cgggtcggac agctccatgt tgtagcggtc ctggtagcgc
     4501 tggaacagct tgcgtttgat cacccagtcg atctcggtgt ctaccttcgc gaagtcctgg
     4561 ctctcgacgg cgtcgagctg gcgtccccac aggtcgacga cctgctcgat ctgcgggttc
     4621 ggctcgcggg tctgcaggtg ctcgacggct cgcgagtggt actcgcgctg gatgtccagg
     4681 gcgctggcct gacggccgcc cgccagccgc acgggccgcc ggccggtgag gtcgtggctg
     4741 acctcgcgga tcgcccggat cgggttgtcg agggagaagt cgcggaacgc cacgccggac
     4801 tcgatcatct ccagcaccag cgcggccgtg cccaccttga gcatggtggt ggtctcgctc
     4861 atgttggagt cgccgacgat gacgtgaagg cgccggtatt tctcggcgtc ggcgtgcggt
     4921 tcgtcacggg tgttgatgat cgggcggctg cgggtggtcg ccgacgacac gccctcccag
     4981 atgtgctcgg cgcgctggct caggcagaag gtggcggcct tcggggtctg cagcaccttg
     5041 cctgcgccgc agatcagctg gcgggtcacc aggaacggca gcaacacatc ggagatgcgg
     5101 gagaactcgc ccgcccgcac gatcaggtag ttctcgtggc agccgtatga gttgcccgcc
     5161 gagtcggtgt tgttcttgaa caggtagatg tcgccgccga tgccctcgtc ggccagccgc
     5221 tgctcggcgt cgatcagcag gtcctcgagg acgcgctcgc ctgcgcggtc gtgggtgacc
     5281 agttgcacga ggctgtcgca ctcggcggtc gcgtactccg ggtggctgcc gacgtcgagg
     5341 tacaaccgtg cgccgttgcg cagaaagaca ttggagctac gcccccacga caccacgcgt
     5401 ctaaacagat agcgtgcgac ctcgtcaggg ctcagccgtc gatgcccatg aaacgtgcag
     5461 gtcacaccga attcggtctc gatgcccatg atccgtcgct gcacctcatc gagcttacgg
     5521 ggtggtttcg aatcgcggcg catcagccgc gccgttagcg ccccattgtc tacagagccc
     5581 gccggggtcc cgaggatccg cgtcgcacgg agacgcctgt ggataacatc agcgccacag
     5641 cgcagtgatc tgccagcatg aggcatgggg ggaaacaccg aagtcgatct caatgcgctg
     5701 cgcgccgtcg ctgagcacgt cacggaagca gcggacggga tcgccgaatt gcactggccc
     5761 gcagtggcgg ccgacgagct gaaaggctcg gccgtcggca atgttcccgc gcctgatctg
     5821 atcgcggcga agctcgccga cgtcatcgcc aacatgcgcg ggtgggcggt ggcggcacgg
     5881 atggcggccg atgccttcga gcgggccgac cgcggtagtg ccgagcgctt ccctcgccga
     5941 tgacccggcc gacggtcgca caggccgagg cgtggaatcc cgaggcgctg cgcacggccg
     6001 ccggcgaatg ggacgcagcc gcaaccgatg tgcagtcccg catcgacgcc g
//
LOCUS       DASYLX010000666         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3215664,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000666 DASYLX010000000
VERSION     DASYLX010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215664"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGP27_15805"
     CDS             complement(<1..360)
                     /locus_tag="VGP27_15805"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEV7852797.1"
                     /translation="MADANRARWQQSTSEGIDMKYEEFIARVAEYADLSKEDAETLTR
                     ATLATLAERITGGEAEDLAAQLPGPLKTALTGAPENAEAFSFEEFVERTAERAGTDVD
                     VAEVAVIAVFATLRDAVT"
     gene            complement(667..1032)
                     /locus_tag="VGP27_15810"
     CDS             complement(667..1032)
                     /locus_tag="VGP27_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7852798.1"
                     /translation="MRDSDRAAGQSESVRVDVQRFPSGKCVVRIMGELIRDATSQMIR
                     IVEEELARVPSPLVLDLSAVTDINANGVDALVSAATLAGESDISLCLVGVEGRPTGDA
                     LAAADLTELFEVVSPSDYA"
     gene            complement(1304..>3164)
                     /locus_tag="VGP27_15815"
     CDS             complement(1304..>3164)
                     /locus_tag="VGP27_15815"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEV7852799.1"
                     /translation="REDVVLRNHGAEPVGVTVTVAADVDFADLFAVKEGRAHVPHGVG
                     VDIGAASLTFTHPGSDDTSCAVITATGEPMVTAGRFTFRTVVPARSQWSTCFAVEATI
                     GGRRLRPRHICGEPIETSAPARRLQAWRLSNPRISTPDNGLARSIEAGARDLGALQIE
                     DPCHPDCPVVAAGAPWFMALFGRDSLLTGWMTLALDPRLALCTLRTLARLQGVRVDPM
                     TEEEPGRILHEVRLGREAALALGGGTTYYGTADATPLFVMLLGELDRWGGLPDTDRPA
                     LVAAADRALKWIGEYGDADGDGFVEYRRHTDRGLVNQGWKDSFDGVTHADGHLAAPPI
                     ALCEVQAYVYGAYRARAGLAEADGDATTARQWRSKAARLKAEFNLAFWMPERQAFALA
                     LDGSKQQADAITSNMGHCLWTGIVDADKAAPVAAHLAGPALCSGFGVRTLATDMAAYN
                     PMSYHNGSVWPHDTAINVAGLARYGYLAEAQRISIGLLEASQHFGGRLPELFCGFDRT
                     DFPSPVPYPTSCSPQAWAAAASRLVLRSLLQLNPNVPHGEVHLAPALPARWLPLRVEN
                     IPLAGKRFGITVDQDGDPQVTGLPAEFRLVTNQLRTASGSDIPAQDHQPGRRP"
BASE COUNT          542 a         1016 c         1021 g          585 t
ORIGIN      
        1 tgtgactgcg tcacgcagcg tggcgaatac ggcgattacg gccacctcgg ccacgtcaac
       61 atcggtgccg gcgcgttcgg cggtccgttc gacgaattct tcgaaactga atgcctcggc
      121 attctccggg gcgccagtca gcgcggtctt cagggggccg ggtaactggg ctgccaggtc
      181 ttccgcctca ccaccggtga ttcgttccgc gagagtggcc aacgtggcgc gggtcagtgt
      241 ctccgcatcc tcctttgaca gatcagcgta ctctgccaca cgggcgatga actcttcata
      301 tttcatgtcg attccttccg atgtggactg ctgccatcgt gctcgattcg cgtccgccat
      361 gcctccatct ggccgagccg aattgacggg cgaaaatcgg cctgttaggt gaagtcgtcg
      421 cccgacaccc agcgccgcac gccgccggac aagacgtttg gagaaactgc ggcttatccg
      481 ttatcccaca tgcctgcaac gacattgggg accgaccgac catcgccgag ccgggcttcc
      541 gcgatcggcg cgagtaggtt gcgggtcatg gacgttttcg gtgctgaatc gaactgtcga
      601 atcacagaaa gcgttgcgcc cattcactga tctggacgca atcaggtgcc gagcacacgg
      661 atcgattcat gcgtagtccg atggtgagac gacctcgaac agttccgtca agtcggcggc
      721 cgcgagggcg tcccctgtcg gacgcccctc aacgccgacg aggcagagtg aaatgtctga
      781 ttccccggcc agcgtcgctg ccgatacgag cgcatcgacg ccgttggcat tgatgtcggt
      841 gacggccgac aggtcgagta ccaagggcga agggactcgc gccagctctt cttccacgat
      901 ccgaatcatc tgcgacgttg catcgcggat cagctcgccc atgatgcgca caacgcattt
      961 gccggacggg aaccgttgca catcgacgcg gacggattcg gactgtcccg cagctctgtc
     1021 gctgtccctc acttcacctc caggcgccca agatgttgca tcgagtcaaa gtggcggtca
     1081 gcaatgcgat atctgtgaag cgctctcagc gttgtccgct tcctcgactt ctaccgatgt
     1141 cgttgtgatg acgatcgacg ttactcagtc ggctcgcgag cacgctgacc ctattgcgtg
     1201 ctcacgccgg ggctctgtcg tggtgccagt cgaaggcaag gccatggacg aaagatcggc
     1261 agccggcgaa ttgggtggtg tgcgcaccct ggccccttca gagtcacggt cggcgaccgg
     1321 gctggtgatc ttgtgccggg atatccgagc cggaggctgt ccgaagttgg tttgtcacca
     1381 gccgaaattc agcaggtagg ccggtgactt gcgggtcacc gtcctggtcc actgtgatgc
     1441 cgaaccgctt gccggccagt ggaatgttct ccactcgaag cgggagccat cgggcgggca
     1501 acgcgggggc cagatgcacc tccccgtgcg ggacgttcgg gttcagctga agcaggctac
     1561 gaaggaccag ccgcgacgcc gcggcggccc acgcctgcgg tgaacatgag gtcgggtagg
     1621 gaacggggct gggaaagtcg gttcggtcga acccgcaaaa cagctcgggt agccggcctc
     1681 cgaaatgctg ggatgcctcg agcaacccga tcgagatccg ttgcgcctct gcgaggtaac
     1741 cgtagcgcgc caaccccgcc acgttgatgg ccgtgtcatg cggccacacc gagccgttgt
     1801 ggtagctcat cgggttatag gcggccatat cggtggccaa cgtgcggacc ccaaatccgg
     1861 agcacagcgc cggcccggcc agatgagccg ctacgggcgc cgccttgtcc gcgtcgacga
     1921 ttcccgtcca gaggcagtgg cccatattgg aggtgatggc atccgcctgc tgtttcgagc
     1981 catccagggc caacgcgaat gcttggcgtt cgggcatcca gaacgcgagg ttgaattccg
     2041 ccttcagtcg tgccgccttg ctccgccatt gtcgggctgt ggtggcatcg ccgtcggcct
     2101 cggccaaacc ggcgcgcgcc cggtaggcgc catacacgta tgcctgtacc tcgcacagcg
     2161 cgatcggcgg ggcggccaga tgtccatcgg cgtgtgtgac gccgtcgaag gagtctttcc
     2221 agccctggtt gaccaaccca cggtcggtgt gccggcggta ttcgacgaag ccgtccccgt
     2281 cggcatcacc atattccccg atccacttca aggcccggtc cgccgcagcg acaagcgccg
     2341 gacggtcggt gtcgggtagc ccgccccagc ggtcgagttc gccgagcagc atgacgaaca
     2401 atggggtggc gtcggcggtg ccgtagtagg tggtgccgcc gcctagcgcc aacgccgctt
     2461 ctcggccgag tcggacttcg tgcagtatcc ggccgggttc ctcttcggtc atgggatcca
     2521 cgcggacgcc ctgtagccgg gccaaggtgc gcagcgtgca cagcgccaga cgaggatcca
     2581 aggcgagcgt catccatccg gtcaacagcg aatcgcgtcc gaacaacgcc atgaaccacg
     2641 gagcccctgc ggccacgacg gggcagtcgg gatggcacgg gtcttcaatc tggagggctc
     2701 cgaggtcgcg ggcgcctgcc tcgattgacc tcgctaaccc gttgtcgggc gtgctgattc
     2761 gggggttcga cagtcgccac gcctgtaatc ggcgcgcggg tgcgctggtc tcgatcggtt
     2821 cgccgcaaat atgtcgcggc cggagccgtc gaccaccgat ggttgcttcc acggcgaagc
     2881 aggtcgacca ctgggaccgg gcgggcacca ccgtacggaa ggtgaatctg ccggctgtga
     2941 ccatcggttc gcccgttgcg gtgatcacgg cgcaactagt gtcgtctgag ccggggtgcg
     3001 tgaatgtcag tgacgccgcc ccaatgtcga cccccacccc gtgcggcacg tgcgcgcgtc
     3061 cctctttcac cgcgaagagg tcggcgaagt cgacgtcagc ggcgactgtc acggtcacac
     3121 cgaccggttc tgcaccgtgg ttgcgcagca ccacgtcctc gcgc
//
LOCUS       DASYLX010000667         6750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3655133,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000667 DASYLX010000000
VERSION     DASYLX010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655133"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="VGP27_15820"
     CDS             complement(<1..90)
                     /locus_tag="VGP27_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7852800.1"
                     /translation="MSEPYYITTAIAYPNGDPHIGHAYEYIATD"
     gene            116..958
                     /locus_tag="VGP27_15825"
     CDS             116..958
                     /locus_tag="VGP27_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV7852801.1"
                     /translation="MSTTRPAPPAPEPLSPLVDAHTHLDACGAVTADDVRAIVDRAAA
                     VGVQAVVTIADDLDSARWVTEAADWDPRVYAAVALHPTRANALTDEAKTVIERLAGHP
                     RVVAIGETGMDNYWPGRLDGCATLSEQRDAFAWHIELAKRTGKPLMIHNRDADADVLD
                     VLRAEGAPPTVIFHCFSSDAEMARTCVDAGWVLSLSGTVSFRNARALREAAALIPPGQ
                     LLVETDAPFLTPHPYRGAPNEPYCLPYTVRALADMVDRPAVDVARNTSATAARVYGFS
                     VNAP"
     gene            1062..2189
                     /locus_tag="VGP27_15830"
     CDS             1062..2189
                     /locus_tag="VGP27_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEV7852802.1"
                     /translation="MNALTKLHESRSPHLRALVLVTLLALIFAGGFAVAAHKTVTLTV
                     DGTQMTVATMKSRVIDVITENGYDVGDRDDLYPAGDQAVHQSDTIVLRRSRPLQLSLD
                     GQNSREVWTTASTVDEALAQLAMTDKAPAAASRGSRVPLGGMSLPVVSAKNVQINDGG
                     AVRTVHLAAPNVAGLLAAAGAPLEQSDTVVPAAASPVVENMQIQVTRVRIEKVTERVP
                     LPPANQRVEDPTMNMSRQVVENPGTPGIQDVTFAIAKVNGTETGRLPVANVIVAPSVD
                     GVLRVGTKPGTEVPPVSNGGTWNALAQCEAGGNWGINTGNGFFGGVQFDQNTWERNGG
                     LRYAARADLATREEQITIAEVTRARQGWGAWPVCSGRVGAS"
     gene            2186..3091
                     /gene="rsmA"
                     /locus_tag="VGP27_15835"
     CDS             2186..3091
                     /gene="rsmA"
                     /locus_tag="VGP27_15835"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV7852803.1"
                     /translation="MTIRLLGRTEIRHLAKELDFRPRKSFGQNFVHDANTVRRVVSAS
                     GINRQDHVLEIGPGLGSLTLALLDRGAHVTAVEIDPVLAGQLPHTVAEHSHSEINRLS
                     VLHQDVLELQPGDLPEEPTALVANLPYNVAVPALLHLLADFPSIRTVMVMVQAEVAER
                     LAAEPGGKDYGVPSAKVRFYGKVRRHGMVSPTVFWPIPRVYSGLVRIDRYETTPWPTD
                     PGFRQQVFNLIDIGFAQRRKTSRNAFAEWAGSGNESARRLLAASIDPSRRGETLSIAD
                     FVRLLERSGVWDPQTKRVIQPVELP"
     gene            complement(3095..3889)
                     /locus_tag="VGP27_15840"
     CDS             complement(3095..3889)
                     /locus_tag="VGP27_15840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7852804.1"
                     /translation="MLVVGGDFAGYVIDATLGRGGHATVYRAHRPAGPDRAVALKVLD
                     EHHRGEEQIARLRREFEFAHQLDHPHIVTVYDSGVGWLTMEIIDGGTSSALPNTQTRL
                     TALRQIAAALDYTHRCGIIHCDVKPSNILVSHDFSRAVLIDFGIACAVAETVGWRAKH
                     IEASLPYTAPELLRGRPPSALTDQYALACTAVELLLGAPPYSGAGTWMQLVDAHLNRP
                     VPSYSDKIAWVPRRFDSWLHRALAKLPEARYDSCTELIEQLTRALE"
     gene            3963..4934
                     /locus_tag="VGP27_15845"
     CDS             3963..4934
                     /locus_tag="VGP27_15845"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV7852805.1"
                     /translation="MSNGNTASEWVPTGSVTVRVPGKVNLYLAVGDRRDDGYHELTTV
                     FHAVSLLDEVTVRTADMLSLEILGEGSEGPGALPTDERNLAWQAAELMAEHVGRGPDV
                     AITIEKSIPVAGGMAGGSADAAAVLVAMNSLWELGVPRRDLHSMAAELGSDVPFALHG
                     GTALGTGRGEELATVLTRNTFHWVLAFADGGLSTPAVFAEADRLREAQDRTLPPRLDD
                     AEPVLAALASGDPASLAPLLGNDLQPASLSLDPGLRRTLRAGVEAGALAGIVSGSGPT
                     CAFLCASATSAVEVGAELAGAGVCRTVRVASGPVQGARVVPAPTPQV"
     gene            5191..>6750
                     /locus_tag="VGP27_15850"
     CDS             5191..>6750
                     /locus_tag="VGP27_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7852806.1"
                     /translation="MSRFTDKMYRSARDATTGMVTGEPHEPIRHTWGEVHERARQIAG
                     GLAAAGIGRGDAVGVLAGFPVEIAPTAQGLWMRGASMTMLHQPTPRTDLVVWAEDTMN
                     VVQMIEAKAVIVSEPFMIAIPVLEEQGIKVLTVTDLLASDPIDPIEVGEDDLALMQLT
                     SGSTGSPKAVQITHSNIHSNAEAMFIGAKYDVDRDVMVSWLPCFHDMGMVGFLTIPMY
                     FGAELVKVTPMDFLRDTLLWARLIDKYKGTMTAAPNFAYALLAKRLRRQAQPGEFDLS
                     TLRFALSGAEPVEPADVEDLLDAGKPFGLEPSAILPAYGMAETTLAVSFSECNAGLVV
                     DEVDRDLLAALRRAVPATKGNTRRLATLGPLLEGLEARIIDEQGNVMPTRGVGIIELR
                     GEPVTPGYITMGGFIPAQDEQGWYDTGDLGYLTDQGHVVVCGRVKDVIIMAGRNIYPT
                     DIERAAGRVEGVRPGCAVAVRLDAGHSRETFAVAVESNAWEDPAEVHRIERQVAHEVV
                     GEVDVRPRNVVV"
BASE COUNT         1110 a         2228 c         2315 g         1097 t
ORIGIN      
        1 atcggtcgcg atgtattcgt aggcatggcc gatgtgcgga tcgccgttgg gataggcgat
       61 cgctgtcgtt atgtagtacg gctcgctcat ttctccctca gcctatggtg tggcggtgag
      121 caccacccgt ccggcgccgc cggctcccga gccgttgagt ccgttggtgg acgcgcacac
      181 ccacctcgac gcgtgcggcg ccgtcaccgc cgacgatgtg cgcgcgatcg tggaccgcgc
      241 cgcggccgtc ggcgtgcagg ccgtcgtcac catcgccgac gacctggatt ccgcccgctg
      301 ggtcaccgag gccgccgact gggatccgcg cgtgtatgcg gccgtggcgc tgcacccgac
      361 acgggccaac gcgctcaccg acgaggcgaa gacggtcatc gaacggctgg cgggccatcc
      421 ccgcgtggtg gcgatcgggg agaccggcat ggacaactac tggcccggcc gactcgacgg
      481 gtgcgcgacg ctgagcgagc agcgcgacgc cttcgcctgg cacatcgagc tggccaagcg
      541 gaccggcaag ccgctgatga tccacaaccg cgacgccgac gccgacgtgc tcgacgtgct
      601 gcgggcggag ggcgcgccgc cgacggtgat cttccactgc ttctcttcgg acgccgagat
      661 ggcgcggacg tgtgtcgacg cgggctgggt gctgagcctg tcgggcaccg tcagcttccg
      721 caacgcccgc gcgctgcgcg aggcggccgc actcatccca cccggacagc tgctggtgga
      781 gaccgacgcg ccgtttctga ctccgcaccc ctaccggggc gcgccgaatg agccttactg
      841 cctgccgtac accgtgcgcg cactcgccga tatggtggac cggcccgccg tcgacgtggc
      901 ccggaacacg tcggcaaccg cagcccgcgt atacggcttc tctgtgaacg cgccgtgaac
      961 ggtgaccgag ttgctgttgc tgagcgggtt cgttaccgtc ttgtgatcga tggcgggggc
     1021 gctgaagcag accggggtcc gagtcgggga attgcactga tttgaatgca ttaacgaaac
     1081 tccacgagtc gcgctcgccg cacctgcggg cactggtact cgtcacattg ctggcgctca
     1141 tcttcgccgg cggcttcgct gtcgcggcgc acaaaaccgt gacgctgacc gtcgacggca
     1201 cccagatgac cgttgcgacg atgaaatctc gcgtcatcga cgtcatcacg gagaacggct
     1261 acgacgtcgg cgaccgcgac gatctctacc cggcaggcga ccaggcggtg caccagtccg
     1321 acaccatcgt gctgcggcgc agccggcctc tgcagctctc gctggacggc cagaacagcc
     1381 gcgaggtgtg gaccacggcg tccaccgtcg acgaggcgct ggcccagctt gcgatgaccg
     1441 acaaggcgcc cgcggcggcc tcccgcggca gccgagtacc gctcggcggc atgtcgttac
     1501 cggtggtgag cgccaagaac gtgcagatca acgacggtgg cgccgtgcga accgtgcatc
     1561 tggccgcgcc gaacgtcgcc ggcctgctgg ccgccgcagg cgctccgctg gaacagagcg
     1621 acacggtggt gcccgccgcc gcatccccgg tcgtggagaa catgcagatt caggtgacgc
     1681 gcgtccgaat cgagaaggtt accgagcgcg taccgttacc gccggccaac cagcgggtcg
     1741 aggatccgac gatgaatatg agtcgccagg tcgtcgaaaa ccccggaact ccgggaattc
     1801 aggacgtaac gtttgccatc gccaaggtca atgggaccga aaccggcaga ttgccagtag
     1861 ccaatgtcat tgtcgcgccg tccgtcgacg gggtgctgcg cgtgggtacg aagccgggta
     1921 ccgaggtgcc tccggtgagc aatggcggca cctggaatgc gctggcgcag tgtgaggcgg
     1981 gtggcaactg ggggatcaat accggcaacg gatttttcgg tggcgtgcaa tttgatcaaa
     2041 atacctggga gcgaaacggt ggtctgaggt atgctgcgag ggccgatttg gcaacgcgcg
     2101 aagagcagat cacgattgct gaggtcaccc gggcccgtca aggatggggc gcgtggcccg
     2161 tatgtagcgg gagagtgggg gcgtcctgac aatcagactg ctgggccgga ctgaaattcg
     2221 gcacctggcc aaagagcttg attttcggcc gcgcaagtct ttcggccaga acttcgtcca
     2281 cgacgcgaac actgtgcgcc gtgtggtctc ggcgtccggg atcaatcgtc aggaccatgt
     2341 cctggagatc ggccccggcc tcggctcgct gactctcgcg ctgcttgatc gcggcgcgca
     2401 cgtcaccgcc gtggagatcg accccgtact ggcgggtcag ctgccgcaca ccgtcgccga
     2461 acactcgcac agcgagatca acaggctttc ggtgctgcac caagacgtgc tcgaactgca
     2521 gcctggcgac ctgcccgaag agccgacggc cctggtcgcg aacctgccct acaacgtcgc
     2581 cgtgcccgca ctgctgcatc tgctcgccga cttcccgtcg atccggaccg tcatggtgat
     2641 ggtgcaggcc gaagtggccg agcgtctcgc ggccgagccc ggcggcaagg attacggcgt
     2701 gcccagcgcg aaggtcaggt tctacggcaa ggtgcgcagg cacggcatgg tgtcgccgac
     2761 ggtgttctgg ccgatcccgc gggtgtactc ggggctggtc cgcatcgacc ggtacgagac
     2821 cacgccgtgg ccgacggatc ccggcttccg ccagcaggtg ttcaacctga tcgacatcgg
     2881 tttcgcccag cgtcgcaaga cgtctcgcaa cgcattcgcc gaatgggcgg gcagcggcaa
     2941 cgagtcggcg cgacggctgc tggccgccag catcgacccg tcgcgacggg gcgagacctt
     3001 gtccatcgcc gacttcgtga ggctgctgga gcgctcgggg gtctgggacc cccagacgaa
     3061 aagggtgatc cagccggtcg agttgcccta gccgctactc cagcgcgcgg gtgagctgct
     3121 cgatgagctc cgtgcacgag tcgtatcggg cctcggggag tttcgccaac gcgcgatgca
     3181 accacgagtc gaaacgacgc ggtacccagg cgatcttgtc cgagtagctt ggcaccggcc
     3241 ggttgagatg agcgtcgacg agttgcatcc aggtgcctgc ccctgagtaa ggcggcgcgc
     3301 cgagcagaag ctcgaccgcc gtgcacgcca acgcgtactg atccgtcagg gccgacggcg
     3361 gcctgccgcg cagcagctcc ggcgccgtgt acggcagcga ggcctcgatg tgtttcgcac
     3421 gccaccccac ggtctccgcg accgcacatg cgatcccgaa gtcgatgagc acagcacggg
     3481 aaaagtcatg cgagacaaga atgttggacg gtttcacgtc gcaatggatg attccgcacc
     3541 ggtgcgtgta gtccagcgca gcggcgatct ggcgcagcgc ggtcagcctg gtctgcgtgt
     3601 tcggcagtgc gcttgacgtg ccaccgtcga tgatctccat ggtgagccag ccgacgccgc
     3661 tgtcgtacac cgtcacgatg tgtgggtggt cgagctggtg tgcgaactcg aattctcttc
     3721 gtagccgggc gatttgctcc tcaccgcggt ggtgctcgtc tagaacctta agcgccaccg
     3781 cacgatcggg gccggcaggc cggtgggccc ggtacaccgt ggcgtgtccg ccccggccga
     3841 gcgttgcgtc gatgacataa cccgcgaagt caccgccgac cacaagcacg attccagcgt
     3901 aggccgggct tttctggccg catgactatt tgttcgcggt gcctcggcgc tagggtcgtg
     3961 ccgtgtccaa cggcaacacc gcatccgagt gggttcccac cgggtcggtc accgtgcggg
     4021 tgccgggcaa ggtcaatctc tatctggccg tcggtgatcg ccgcgacgac ggctatcacg
     4081 agctgaccac cgtttttcat gcggtatcgc ttctcgacga ggtcaccgtc agaaccgccg
     4141 acatgctctc gctggagata ctgggagagg gcagcgaggg cccgggggcg ctgcccaccg
     4201 acgagcgcaa cctggcctgg caggccgccg agctgatggc cgagcatgtc ggcagggggc
     4261 ccgacgtcgc gatcaccatc gagaagtcga tcccggtcgc gggcggaatg gcaggcggaa
     4321 gcgccgacgc ggcggccgtg ttggtcgcga tgaactcgct gtgggagctc ggtgtgccgc
     4381 gccgcgacct gcattcgatg gccgccgaat tgggcagtga tgttccgttc gccctgcacg
     4441 gaggcaccgc actgggcacc ggccgcggtg aggagctggc gaccgtgctg accagaaaca
     4501 cctttcactg ggtgctggcg ttcgccgacg gcggcctgtc cactccggcc gtgttcgccg
     4561 aggccgaccg gctgcgcgag gcgcaggacc ggaccctgcc gccgcgcctg gacgatgccg
     4621 aaccggtgct ggcggccctt gcctccggcg atcccgccag ccttgcgccg ctgctgggca
     4681 acgacctgca gcccgcgtcc ctgagcctcg atcccgggct gcgccggacg ctgcgggcag
     4741 gcgtcgaggc aggcgcgctg gccgggatcg tgtccggttc gggcccgacc tgcgcgtttc
     4801 tgtgcgcctc ggccacctcg gccgtcgagg tcggcgccga gttggccggc gccggggtgt
     4861 gccgcacggt gcgggtggca agcgggccgg tgcagggtgc ccgggtggtt cctgcgccca
     4921 ctccacaggt gtgacacaaa cctcaattac tgcatgagtt tggtagttac ttaagagttg
     4981 cataagatga ctagcggtga aaaatcgcgg tcaaggagtg gacgcaaata tcgccgctgc
     5041 cgggagtccc ggcgagcggg cgtgtcgccg taccgcatca ccgaatcgag acataactgc
     5101 gccacaaacg tatgcgcgcc gacgtgaaag cgcctatgag caggcggagc attacacctt
     5161 ggcatttgcg gggaccccga ggaggctgtc gtgagcagat tcaccgacaa gatgtaccgc
     5221 agtgcccgtg acgccactac cggcatggtc accggagaac cgcacgagcc catccgccac
     5281 acctggggcg aggtgcacga gagggcccgc cagatcgcgg gtggcctcgc cgccgcgggc
     5341 atcgggcgtg gcgacgccgt cggcgtgctg gccggcttcc ctgtcgagat cgccccgacc
     5401 gcccagggcc tgtggatgcg tggcgccagt atgaccatgc tccatcaacc cactccgcgc
     5461 accgacctcg tggtctgggc cgaggacacg atgaacgtcg tccagatgat cgaggccaag
     5521 gccgtcatcg tctccgaacc gttcatgatc gcgattcccg tgctcgagga gcagggcatc
     5581 aaggtgctga cggtgaccga tctgctcgcg tccgatccga tcgacccgat cgaggtcggt
     5641 gaggacgacc tggcgctgat gcagctgacg tcgggctcaa ccggatcgcc gaaagccgtg
     5701 cagatcaccc acagcaacat ccactccaac gccgaggcga tgttcatcgg cgccaagtac
     5761 gacgtcgaca gggacgtcat ggtcagctgg ctgccctgct tccatgacat gggcatggtc
     5821 ggcttcctga ccattccgat gtacttcggc gccgagctgg tcaaggtcac gccgatggac
     5881 ttcctgcgcg acaccctgct gtgggcgcgg ctgatcgaca aatacaaggg cacgatgacc
     5941 gcggcgccga acttcgcgta cgcgctgctg gccaagcggc tgcgcaggca ggcccagccg
     6001 ggtgaattcg acttgtcgac gctgcgcttc gcgctgtcgg gcgccgagcc cgtcgagccg
     6061 gccgacgtcg aggatctgct cgacgcgggt aagccgttcg ggctggagcc ctcggcgatt
     6121 ctgcccgcct acggaatggc cgagacgacg ctggcggtgt ccttctccga atgcaacgcc
     6181 ggcctggtgg tcgacgaggt cgaccgcgac ctgctggccg ctctgcggcg cgcggtgccc
     6241 gcgacgaagg gcaacacccg caggctggcc acgctcggcc cgctgctcga ggggctcgag
     6301 gcccgcatca tcgacgagca gggcaatgtg atgccgactc gcggcgtcgg catcatcgag
     6361 ttgcgcggcg aacctgtcac gcccggctac atcaccatgg gcggcttcat ccccgcgcag
     6421 gacgagcagg gctggtacga caccggcgac ctcggctatc tgaccgacca gggccacgtc
     6481 gtggtctgcg gtcgcgtgaa ggacgtcatc atcatggccg gccgcaacat ctatcccacc
     6541 gacatcgagc gggccgccgg ccgcgtcgag ggcgtgcggc cgggctgcgc cgtcgcggtg
     6601 cgcctggatg ccgggcactc ccgcgagacc ttcgccgtcg cggtggagtc caacgcgtgg
     6661 gaggacccgg ccgaggtgca ccgcatcgag cgtcaggtgg cccacgaggt cgtcggcgag
     6721 gtcgacgtcc ggccacgcaa tgtcgtggtg
//
LOCUS       DASYLX010000668         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3766400,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000668 DASYLX010000000
VERSION     DASYLX010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766400"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGP27_15855"
     CDS             <1..425
                     /locus_tag="VGP27_15855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852807.1"
                     /translation="NAYDILPDDGRMTIQSSTGYHPDDLRERGKKLTFDLARFIKFII
                     TEIFPGGRIPTAKMMVEHGEKAGFVVPECLSLQEHYVRTLRTWADTLEAKKDTVLQVT
                     TEEVYDRYMRYLRGCQNYFADEMLDCNLVTYLKPGAAA"
     gene            complement(440..2311)
                     /locus_tag="VGP27_15860"
     CDS             complement(440..2311)
                     /locus_tag="VGP27_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7852808.1"
                     /translation="MESAPVHSAARPRAGRPKTDPYLDENGRIALPDGMNLSALLDRN
                     IAVFGDVLAYRFLDYSHLPDGGKPLELTWEQVGTRSRAIGARLQQVTARGDRVALLCP
                     QGLDYVTSFFAAIQAGSIAVPLFAPELPGHAERLDAVLTDALPAVVLTTTSASDAVQR
                     FVRLMPREKRPRVIAVDAIPDSVGSTFAPVELDSDDIAYLQYTSGSTRVPTGVEISHR
                     AAGTNFLQMVFAIGLEGDFEINGASWLPLYHDMGLMMVGFPVLYGGNLTFMSPMAFVR
                     RPGRWIQMLHATSRYSHIVSPAPNFAFELVAQRGLPRDNEIDLSNVIFINGSEPVSLD
                     SITKFTDAFLPHGLSKTAVRPSYGMAEATLFVSSIRPYTEPTAVYLDREQLGAGRAVQ
                     VEPGTPNALPQVSCGQVSVSQWAAVVDPESGQELPDDCVGEIWLHGNNIGRGYWAHPE
                     ETEFTFGNKLQARLDTGSHADGADADATWLRTGDLGVYIDGELYITGRIKDMVIVDGR
                     NHYPQDIEATVAESSHAIRAGFVAAFSVPARELPGVSATDTSEKLVIVAERGPGAGRA
                     DPQPILDAVRRAVSRKHSVNVYDTRLVAAGVIPRTTSGKLARRACRAEYLDGSLGRH"
     gene            2589..3452
                     /locus_tag="VGP27_15865"
     CDS             2589..3452
                     /locus_tag="VGP27_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEV7852809.1"
                     /translation="MTKELTPHFGEVQHHYDLSDDFYRLFLDPTQTYSCAYFERDDMT
                     LQEAQLAKIDLALGKLNLQPGMTLLDIGCGWGATLLRAIERYDVDVVGLTLSENQAKH
                     LQRTFDEMESPRSRRVLLEGWERFDEPVDRIVSIGAFEHFGSDRYRAFFAKAYDLLPE
                     DGVMLLHTIVRATLEERRRRGVETTAELVHFSRFILTEIFPGGWLPTIPVVEEHAVRA
                     GFDLSRVQSLQMHYARTLDIWAAALESKRDEAITIQSLQVYDRYMKYLTGSAELFRRG
                     VTDVCQFTLAK"
     gene            3615..>3938
                     /locus_tag="VGP27_15870"
     CDS             3615..>3938
                     /locus_tag="VGP27_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7852810.1"
                     /translation="MHYFALLISPERSLTPEEGAAEMTAYQDFHATAAAAIRAGDALG
                     PAATGVRITGGPDSPTVTDGPFAEGAEVAGGYYVFEADNLDEALALAGDIPAAKYGAV
                     EVWPMV"
BASE COUNT          717 a         1224 c         1298 g          699 t
ORIGIN      
        1 agaacgccta cgacatcctg cccgacgacg gccggatgac gatccagagc agcaccggct
       61 accaccccga tgacttgcga gagcgcggaa agaagctgac gttcgatctg gcccggttca
      121 tcaagttcat catcaccgag atcttccctg gcggccgcat cccgaccgcc aagatgatgg
      181 tggaacacgg tgagaaggcc ggcttcgttg tgcccgagtg cctctccctg caggagcatt
      241 acgttagaac tctgcgtaca tgggcggaca ccctggaagc caagaaggac acggtcctgc
      301 aggtgacgac cgaagaggtc tacgacaggt atatgaggta cctgcgcggc tgccagaact
      361 acttcgccga cgagatgctc gactgcaatc tcgtcacgta cctcaagccc ggcgccgcgg
      421 cctgacgtgt ggcaggcgcc tagtgcctgc ccaggctccc gtcgaggtac tccgcccgac
      481 aggcgcgtcg agccagcttg ccgctggtcg tccgcgggat gacgcccgct gcgacgagcc
      541 gggtgtcata cacgttgacg ctgtgtttgc gcgacaccgc gcgacggacg gcatcgagaa
      601 tgggttgtgg gtccgcccgg cccgctccag ggccccgttc ggccacgatc accagttttt
      661 cgctggtgtc cgtcgcggat acgccgggta gctctcgtgc cgggaccgag aaggccgcca
      721 cgaagccggc gcgtatcgcg tgggacgact cggccacggt cgcctcgatg tcctgcgggt
      781 agtggttgcg gccgtcgacg atgaccatgt ccttgatgcg gccggtgata tacagctcgc
      841 cgtcgatgta gacgcctagg tcaccggtgc gcagccaggt cgcgtcggcg tcggcgccgt
      901 cggcatggct gccggtgtcg agccgagcct gcagcttatt accgaaggtg aactcggttt
      961 cctccggatg cgcccagtat ccgcgcccga tgttgttgcc gtgcaaccag atttcgccga
     1021 cgcagtcgtc cggcagttcc tgaccggact ccgggtcgac gacggccgcc cactggctca
     1081 ccgagacctg gccgcacgac acctgtggaa gggcattcgg cgtgccgggt tcgacctgca
     1141 ccgcccgtcc ggcgcccaac tgctcgcgat cgagatacac cgcggtcggc tcggtgtagg
     1201 gacggatcga cgagacgaac agcgtcgcct ccgccatgcc gtaggacggc cggacggcgg
     1261 ttttggagag gccgtgcggc aggaacgcgt cggtgaactt ggtgatggag tcgaggctca
     1321 ccggctccga gccgttgatg aagatgacgt tgctcaaatc gatctcgttg tccctgggaa
     1381 ggccgcgctg ggcaaccaac tcgaaggcga aattcggtgc aggcgagacg atgtgactgt
     1441 accgcgacgt ggcgtgcagc atctggatcc agcgccccgg ccggcggacg aacgccatcg
     1501 gcgacatgaa agtcaggttt ccgccgtaga ggaccgggaa gccgaccatc atcaggccca
     1561 tgtcgtggta gagcggcaac caactggcgc cgttgatctc gaagtctccc tcgagtccaa
     1621 tggcgaagac catttgcagg aagttcgttc cggcggcgcg gtgactgatc tccaccccgg
     1681 tcggcacgcg tgtggagccg gacgtgtact gcagataggc gatgtcgtcg gagtccagtt
     1741 cgaccggcgc aaacgtcgat cccaccgagt cggggatggc gtcgacggca atgacgcggg
     1801 gcctcttctc gcgcggcatc agccgaacga atctctggac tgcatcgctg gcggaggttg
     1861 tcgtcagcac gaccgcaggc agggcgtcgg tcagcacggc atcgaggcgc tcggcgtggc
     1921 caggaagctc cggcgcgaac aacggcaccg cgatgctgcc cgcctgaatc gcggcgaaga
     1981 agctcgtcac gtaatcaaga ccctgcgggc acagcagggc cacccgatca ccgcgagcgg
     2041 tcacctgctg aagccgggcc ccgatggctc tagagcgggt gcccacctgc tcccaggtga
     2101 gctcgagcgg cttccccccg tccggcagat ggctgtagtc gagaaagcgg taggcgagga
     2161 cgtcgccgaa gactgcgatg ttcctgtcga gcagcgcgga gagattcatg ccgtctggca
     2221 gcgcgatgcg accgttctca tccaagtagg ggtcggtttt gggccgaccc gctcttggac
     2281 gggcagcgct atgcacagga gcactttcca taagcgcagt ctaagagatg agatgcgtca
     2341 gtcgtgaatc ggcgcggtcg aatcgttgag attgaagtgt cccttcgccg agccgatctg
     2401 gggaaacatg acaattgtct ttacttctga cacatcggca ccaaagcggc agcttcagac
     2461 aaatagatga gacgttctgc catatttttg gggtggccgc gaaacggccg ctttcgcgtc
     2521 tcgacctggg tttcaaacca cgtgacctgg ctattaggtt gcgtatctcc ggggaggaag
     2581 ccgctcacat gacgaaggaa ctcacaccgc atttcggcga ggtgcagcac cactacgacc
     2641 tttccgacga cttctatcgg ctgttccttg accctaccca gacctacagc tgcgcatact
     2701 tcgaacgcga cgacatgacg ttgcaagagg ctcagctggc caagatcgac cttgcgctcg
     2761 gaaagttgaa ccttcagccc ggcatgacgt tgctcgacat cggctgcggt tggggcgcca
     2821 ccctgctgcg cgccattgag cggtacgacg tcgacgtagt cggcctcacg ttatccgaaa
     2881 accaggccaa gcacctgcaa cgcaccttcg acgagatgga aagcccgcgc tcgaggcggg
     2941 tgctgctcga gggctgggag cggttcgacg agcccgtcga ccgcatcgtg tcgatcgggg
     3001 cgttcgagca cttcggcagc gaccgctaca gggcgttctt cgccaaggcc tacgacctgt
     3061 tacctgagga cggcgtgatg ctgctgcata cgatcgttcg cgccacgctg gaggagcgca
     3121 ggaggcgagg cgtggaaacc accgcggagc tggtgcactt ctcccgcttc atcctgaccg
     3181 agatcttccc aggcggttgg ctgccgacga tcccggtcgt cgaggagcac gccgtgcgcg
     3241 ccgggttcga cctgtcccgc gtgcagtcgc tgcaaatgca ctatgcgaga acgctggaca
     3301 tctgggctgc ggcgttggag tccaagcggg acgaagccat cacgatccag tcgctccagg
     3361 tttacgaccg ctacatgaag tacctgacgg gcagcgcaga actgttccgg cggggtgtga
     3421 ccgacgtgtg tcagttcact ctggccaagt agcaccgcga gcgtggggcc ctggcaccgc
     3481 gagctggggg cctcgcgagg gtgcgtgagt gatcgctcgc cgagcgagct gttgtcggat
     3541 tccaagcgtc tcgttcgtcg gacgggtaga gagtggaaca cagggttccg ctcgctatgt
     3601 ggaaggtctc gacgatgcac tacttcgcct tgctgatcag ccccgaacgc tctctcaccc
     3661 cagaggaggg cgcggctgaa atgactgctt atcaagattt ccatgccacg gccgccgcgg
     3721 ccatccgcgc aggcgatgcg ctggggccgg cggcaaccgg tgtgcggatc accggtgggc
     3781 cggactcccc gacggttacc gacggtccgt tcgcggaggg cgccgaagtg gccggcggct
     3841 actacgtttt cgaggccgac aacctcgacg aggcgttggc gctggccggt gacatacccg
     3901 ccgcgaagta cggtgccgtc gaggtgtggc cgatggtg
//
LOCUS       DASYLX010000669         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1240635,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000669 DASYLX010000000
VERSION     DASYLX010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240635"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGP27_15875"
     CDS             complement(<1..390)
                     /locus_tag="VGP27_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV7852811.1"
                     /translation="MTVASGSNVAGHAAPDGRKLLRLEVRNAQTPIERKPSWIKTRMR
                     TGPEYTQLKNLVRREGLHTVCEEAGCPNIYECWEDREATFLIGGEQCTRRCDFCQIDT
                     GKPADLDRDEPRRVAESVAAMGLRYATV"
     gene            complement(387..1061)
                     /gene="lipB"
                     /locus_tag="VGP27_15880"
     CDS             complement(387..1061)
                     /gene="lipB"
                     /locus_tag="VGP27_15880"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEV7852812.1"
                     /translation="MVSAIRSSNTPIDVRRLGTIGYEDAWAVQRELANSRVDGGPDTL
                     LLLEHPSVYTAGRRTLPEERPVDGTAVIDTDRGGKITWHGPGQLVGYPIIGLAEPLDV
                     VNFVRRLEESLIRVCRELGLETGRVRGRSGVWVPGDAQGPARKVGAIGVRVSRATTLH
                     GFALNCDCDLSAFSSIVPCGIADAGVTSLTAELGRHVGVDDVIDRVTAAVCDALDGRL
                     RVALTS"
     gene            complement(1055..1957)
                     /locus_tag="VGP27_15885"
     CDS             complement(1055..1957)
                     /locus_tag="VGP27_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV7852813.1"
                     /translation="MATVAIAGSSGLIGAALVYALRATDHRVLRIVRRVPSNSDELFW
                     NPDTGEFDANALRGVDVAVNMCGVNVGSKRWSGAFKQSLRDSRIDPTEVLSEAVAEAG
                     VGTLINASAVGYYGDTANRIADETAPPGRGFLAQLCTDWEAATAAAQDAGARVVLTRT
                     GLVLSPRGGMLSRLRPLFTLGLGARLGRGRQYMPWISLEDQVRALLFAIFHDELSGPV
                     NFTGPAPVTNAEFTTAMGRAVNRPTPLMVPGFALRTLLGEFAGEGLLGGQRAIPAALE
                     RAGYQFRHNTVGEALAYATSTVES"
     gene            complement(1963..>3154)
                     /gene="sucB"
                     /locus_tag="VGP27_15890"
     CDS             complement(1963..>3154)
                     /gene="sucB"
                     /locus_tag="VGP27_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEV7852814.1"
                     /translation="VGGELAKIGDAEAAPAEKPAPEPEPKEEPKEEPKEEAKKEPEPE
                     PEPEPESKEEAKPEPKPEPKEEAKPEPKPEPQAAEEPSGDGAPYVTPLVRKLAGDNGI
                     DLASVKGTGVGGRIRKQDVLAAAEAKKAPAQEAPAAKEAPAAAAKVETASALAHLRGT
                     TQKANRIRQITAKKTRESLQQTAQLTQTHEVDMTRIVALRARAKATFAEREGVNLTFL
                     PFFAVAVVDALKAHPNVNASYNEETKEITYYDAEHLGFAVDTEQGLLSPVIHNAGDLS
                     LAGLARAISDIAARARSGDLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTG
                     AIVKRPRVVVDSTGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKRRLEEGAFEA
                     DLGL"
BASE COUNT          434 a         1106 c         1059 g          555 t
ORIGIN      
        1 gaccgtggcg taccgcagcc ccatggctgc gacgctctcg gcgaccctgc gcggctcgtc
       61 gcggtccagg tcggcgggct ttccggtgtc gatctggcag aagtcgcacc ggcgcgtgca
      121 ctgctcgccg ccgatcagga acgtcgcctc ccggtcctcc cagcactcgt agatgttggg
      181 gcaaccggct tcctcgcaga cggtgtgcag cccctcgcgg cgcaccaggt tcttcagctg
      241 cgtgtactcc gggcctgtcc gcatccgggt cttgatccac gacggtttgc gctcgatcgg
      301 agtctgcgca ttgcggactt ccaggcgcag cagcttgcgg ccatccggag ccgcatggcc
      361 ggcgacattg gatccagaag cgacggtcac gacgtcaacg ctactcggag ccggccgtcg
      421 agcgcgtcgc acacggcggc cgtgacacgg tcgatgacgt cgtcgacgcc gacgtgacgg
      481 cccagttcgg ccgtcagcga agtgactccc gcatcggcga tgccgcacgg cacgatcgag
      541 gagaacgcgc tcaggtcgca gtcgcagttc agcgcgaagc cgtgcagtgt cgtcgcccgc
      601 gacaccctca ccccgatcgc gccgaccttg cgcgcaggac cctgcgcgtc cccgggcacc
      661 cagacacccg agcggccccg cacgcgcccg gtctcgagcc cgagttcgcg gcacacgcgg
      721 atgagcgact cttcaaggcg ccgaacgaaa ttcaccacat cgagcggttc agccagccca
      781 atgatcggat agccgaccag ctggccgggt ccgtgccagg tgatcttgcc tccgcggtcg
      841 gtgtcgatga cggctgtgcc gtcgaccgga cgctcctcgg gcagcgtgcg gcggcccgcc
      901 gtatagaccg agggatgctc gagcagtagc agcgtgtcgg ggccgccgtc gacgcgtgag
      961 ttcgccagct cccgctgcac cgcccaggcg tcttcgtagc cgatcgtgcc caggcgccgg
     1021 acgtcgatcg gtgtgttgga cgaccggatg gcactcacga ttcgacggta ctcgtggcgt
     1081 acgccagcgc ctcgccaacg gtgttgtggc ggaactgata gcccgcgcgt tccagcgctg
     1141 cggggatggc ccgctgcccg ccgagcaggc cttccccggc gaactcgccc agcagtgtcc
     1201 gcagcgcgaa accgggaacc atcagtggcg tcggccggtt caccgcgcga cccatggccg
     1261 tcgtgaactc ggcgttcgtc accggcgccg gtccggtgaa gttcaccggg ccggacagct
     1321 cgtcgtgaaa gatcgcgaac agcagcgcgc ggacctggtc ttcgaggctg atccacggca
     1381 tgtactggcg gccgcggccg aggcgggcgc ccagaccgag cgtgaacagt ggccgcagcc
     1441 ggctcagcat cccgccgcgc ggcgacagca ccagcccggt ccgtgtcagc acgacgcggg
     1501 cgcccgcatc ctgcgccgcg gccgtcgcgg cctcccagtc cgtgcacaac tgggctagga
     1561 acccgcggcc gggcggcgcg gtctcgtcgg cgatccggtt tgcggtgtcg ccgtagtagc
     1621 cgaccgcact ggcgttgatg agcgtcccga cgcccgcctc ggccaccgct tcggacagca
     1681 cctcggtggg gtcgattcga ctgtcgcgca ggctctgctt gaatgcgccc gaccaccgct
     1741 tggagccgac gttgacgccg cacatgttga cggcgacgtc cactccgcgc agcgcattcg
     1801 cgtcgaactc gccggtgtcg ggattccaga acagctcatc ggaattcgac ggcacccggc
     1861 gcacaatgcg cagtacccgg tggtcggtcg cccgcagcgc atagaccagc gccgcgccga
     1921 tcagccccga ggaccccgca atcgcaacgg tggccatggt gcctacagcc ccaggtccgc
     1981 ctcgaacgca ccctcttcga gccgacgctt gatcgtggtc aggaagcgac ctgcgtccgc
     2041 gccgtcgatc agcctgtggt cgtacgtcaa gggcaggtag cagatcgagc gcacgccgat
     2101 cgactcgttg ccggtgctgt cgacgacgac gcggggacgc ttgacgatcg cacccgtgcc
     2161 cagcatcgcc gcctgcggcg gcaccaggat cggggtgtcg aacagcgcac cctggctgcc
     2221 gatgttggtg atcgtgaagg tgccgccgga caattcgtcc ggtttcaggt cccccgaacg
     2281 ggcccgcgcc gcgatgtcgg agatggcgcg cgccagtccg gccagcgaca ggtcgcccgc
     2341 gttgtgaatc accggcgaca gcaggccttg ttcggtgtcc accgcgaagc cgagatgctc
     2401 ggcgtcgtag taggtgatct ccttggtctc ctcgttgtag ctggcgttga cgttcgggtg
     2461 cgccttgagc gcgtcgacga cggcgacggc gaagaacggc aggaacgtca ggttcacgcc
     2521 ttctcgctcg gcgaaggtgg ccttggccct ggcccgcagc gccacgatcc tggtcatgtc
     2581 gacctcgtgg gtctgcgtca attgcgctgt ctgctgcaga gattcacgcg tcttcttcgc
     2641 ggtgatctgg cggatgcgat ttgccttctg cgtggtgccg cgcagatgcg ccaacgctga
     2701 agcggtttcg accttcgccg cggcggcggg cgcctccttg gcggcgggcg cctcctgtgc
     2761 gggtgccttc ttggcttccg cagcggccag cacatcctgc tttcggatgc ggccgccgac
     2821 cccggtgccc ttcacggaag caagatcgat gccgttgtcg ccggccagct ttcgtaccag
     2881 cggtgtcaca tacggtgcac cgtcaccgga cggctcctcc gcggcctgcg gttcgggctt
     2941 gggttcgggc ttggcctctt ccttgggctc gggtttcggc tcgggcttgg cctcttcctt
     3001 ggactcgggt tccggttccg gttccggttc gggctctttc ttggcttctt cctttggttc
     3061 ctctttcggc tcttccttgg gttccggttc gggcgcgggc ttctcggcgg gcgcggcctc
     3121 cgcatcgccg atcttggcca gttcgccgcc gacg
//
LOCUS       DASYLX010000670         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1791069,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000670 DASYLX010000000
VERSION     DASYLX010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791069"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VGP27_15895"
     CDS             complement(<1..143)
                     /locus_tag="VGP27_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HEV7852815.1"
                     /translation="MAGYVGRDAVIVGAVRTPIGKGKASGALHDVLPVDLLAHSLKEL
                     LNRT"
     gene            239..709
                     /locus_tag="VGP27_15900"
     CDS             239..709
                     /locus_tag="VGP27_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852816.1"
                     /translation="MTVLQGRLADRDAWSAVGECPIEKTMTLLGTKSAMLILREAYYG
                     TTRFDDFCTRVGITKAAASARLNELVDAGLMTKRPYREPGQRAREEYVLTSSGVEFMP
                     VVWAMFEWGRKHLPNRTPLRLAHAGCGAEAGVEIRCAKGHHVPADELVVRAGGR"
     gene            complement(713..2122)
                     /locus_tag="VGP27_15905"
     CDS             complement(713..2122)
                     /locus_tag="VGP27_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEV7852817.1"
                     /translation="MPATDPTFYRTPGAAATAAPEELAYVAAFDPAGRMKDAIAVVDC
                     DPASAGYGGVVGWSELPTAGNELHHFGWNACSSALCHEGHDQHNGLERRYLLVPGIRS
                     STTYVLDTKPDPRNPVLKHTIEAGELAAKAGYSRPHTVHCGPGGIFMSALGGANGEDG
                     PGGIALLDHDTFEVLEPWEKDRGEQFFAYDFWWHLQQDTVVTSEWASPSMIENGLDPE
                     NLLGRRFGHHLNFWSMSERKLSQRIDLGDEHQMVLELRPAHDPSKSYGFASVVISVED
                     LSASIWLWHRDGDQWAVQKVIVIPAEPADAADLPPLLKGFGAVPPLVSDIDLSVDDRW
                     LYVSCWGTGELKQFDVSDPFHPRETASVRLGGIVRREAHPAFPGQPLGGGPQMVEISR
                     DGRRVYVTNSLYASWDEVFYPDGVGSWMAKLDADGQSGGLTADVGFFPHGDDFRGLRV
                     HQTRLQGGDASSDSYCFTT"
     gene            complement(2254..2673)
                     /locus_tag="VGP27_15910"
     CDS             complement(2254..2673)
                     /locus_tag="VGP27_15910"
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEV7852818.1"
                     /translation="MKRRFPLAAALLVAVGVAGCSSPPAALGTRDAKVIIDGKATNAP
                     QPVTCYQTGQSYTIETLDKEPGFTATIQLGDTVTPQVVDIRNLGNFTGIYWDDNLGKA
                     QARVNQNKFSVSGEAQGAFADKPNQSTTATFDITTSC"
     gene            2725..>3107
                     /locus_tag="VGP27_15915"
     CDS             2725..>3107
                     /locus_tag="VGP27_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEV7852819.1"
                     /translation="MLHLRVITPAALRDAVIDALTGNPGVAHIVVHRDAALDPPGDEI
                     TADIARESANDVIEALKALGVKERGAITLDVVDTVLSTRAYRAEEEAAGDPADAVVWD
                     ELAGRTREESTLNVTFLTFLCLACL"
BASE COUNT          546 a          979 c         1027 g          555 t
ORIGIN      
        1 gtgcgattca ggagctcctt caggctgtgg gcgagcaggt cgaccggcag cacgtcgtgc
       61 agtgcgccgc tggccttgcc cttcccgata ggggtgcgta ccgctccgac gatgaccgcg
      121 tcccggccta cataaccggc catttctgcc tcctgcgact cgacgctgag taaagctatc
      181 tatatccagc tataccacct gggtatactg agaacaactc agaagcgagg tgaattccat
      241 gacagtgctg caaggcaggc tggccgatcg agacgcctgg tcagcggtgg gcgaatgtcc
      301 gatcgagaag acgatgacgc tgctcggcac caagtcggcg atgctgatcc tccgggaggc
      361 gtactacggc acaacgcggt tcgacgactt ctgcacccgg gtcggcatca ccaaggcggc
      421 ggcctcggcc aggctcaacg agctggtgga cgcggggctg atgaccaaac ggccctaccg
      481 cgagccgggc cagcgagccc gcgaggaata cgtgctgacg tcctcgggcg tcgagttcat
      541 gccggtggtg tgggcgatgt tcgagtgggg tcgcaagcac ctgccgaacc gaacgccgct
      601 gcggctggcg cacgcgggct gcggcgccga agccggcgtc gagatccgct gcgccaaggg
      661 gcatcacgta ccggccgatg agctcgtcgt gcgcgcaggc ggccgatgac gatcaggtgg
      721 tgaagcagta cgagtcgctc gacgcgtcac cgccctgcag ccgggtctgg tgaacgcgaa
      781 gtccgcggaa gtcgtctccg tgcggaaaga acccgacgtc ggccgtgagc ccaccgctct
      841 ggccgtcggc gtcgagcttg gccatccacg agccgacgcc gtcagggtag aagacctcgt
      901 cccaggacgc gtagagggag ttggtgacat agacgcgcct gccgtcgcgg ctgatctcga
      961 ccatctgcgg accgccgccc aggggttggc ccggaaatgc agggtgagct tcgcgcctga
     1021 ctatcccgcc gagcctcacc gacgccgtct cccgcgggtg gaacggatcg ctgacgtcaa
     1081 attgtttgag ttctcctgtg ccccaacatg atacgtacag ccatcggtcg tcgacagaca
     1141 agtcgatgtc gctgaccagc ggcggtaccg cgccgaaccc cttcaggagg ggcggcaggt
     1201 cggcggcgtc ggctggctca gccgggatga cgatcacctt ctgaaccgcc cactgatcgc
     1261 cgtccctgtg ccacaaccag atcgacgccg acagatcctc gacactgatg acgacgcttg
     1321 caaacccgta ggacttagac gggtcatggg ccgggcgcag ctccaacacc atttgatgct
     1381 cgtcgccgag gtcgattcgc tggctgagct tgcgctccga catgctccag aagttcaggt
     1441 ggtggccgaa tctgcggccc agcagatttt cggggtcgag accattctcg atcatcgacg
     1501 gcgacgccca ttcggaggtc accaccgtgt cctgctgtag gtgccaccag aagtcgtagg
     1561 cgaagaactg ttccccccgg tctttctccc acggttcgag cacttcaaac gtgtcgtggt
     1621 cgaggagcgc aatgccgcct ggtccgtcct cgccgttcgc gccacccaga gcagacatga
     1681 agatgccccc aggcccgcaa tgcacggtgt gcggacggga atagccggcc ttggctgcca
     1741 gctcaccggc ctcaatggtg tgcttcaaaa ccggattacg tggatcgggc ttggtatcga
     1801 ggacataggt ggtcgacgac ctgattcccg gcacgagaag gtagcgccgc tccagaccgt
     1861 tgtgctggtc atggccttcg tggcacaacg cgctcgagca tgcgttccaa ccgaaatgat
     1921 gaagctcgtt tccggccgtc ggcaattcgc tccatccgac cacgccgccg tagccggccg
     1981 aggcggggtc gcaatcaacc accgctatcg cgtccttcat ccggccggcg gggtcgaagg
     2041 cggcaacgta ggcgagttcc tcgggcgcgg cggtcgcggc agcgcccggt gtgcggtaaa
     2101 acgtggggtc tgtcgcaggc atggttcaaa tgatcctccg gcgcggagtc agaaggttct
     2161 gttcgccaga aacgccttga cgagtgcctt cggctactgc gccggccacg cgtcggcgac
     2221 gatgctggcc acgtccggga aggacgccac gtctcagcag ctggtggtga tgtcgaacgt
     2281 cgccgtcgtg ctctggtttg gcttgtcggc gaaggcgccc tgggcctcac cgctgacgga
     2341 gaacttgttc tggttcaccc gcgcctgggc cttgccgaga ttgtcgtccc agtagatgcc
     2401 ggtgaaatta cccaggtttc ggatgtcgac cacctgaggg gtcaccgtgt cacccagctg
     2461 gatcgtcgcc gtgaatcccg gctccttgtc gagtgtctcg atggtgtagc tctggccggt
     2521 ctggtagcag gtcaccggtt gcggcgcgtt ggtggcctta ccgtcgatga tcaccttggc
     2581 gtcacgggtg cccagtgcgg cgggaggact cgagcagccc gccacgccga ctgcgacgag
     2641 gagtgcggct gctaggggaa accggcgctt catgaatgtg acattagagc agcggccgcc
     2701 ggcgaagggc tagtgtcgct gaaaatgctg catctacggg tcatcacacc ggctgcgttg
     2761 cgcgatgccg tgatcgatgc gctgaccggc aatcccggcg tggcacacat tgtggtccat
     2821 cgcgacgccg ccctggaccc gccaggtgac gagatcaccg ccgacatcgc ccgagaatcc
     2881 gccaacgacg tcatcgaagc cctgaaggcc cttggagtaa aggagcgcgg ggcgatcacg
     2941 ctggacgtcg tcgacaccgt gttgtcgacg cgggcctacc gcgccgagga ggaagcggcc
     3001 ggtgatccgg ccgatgcggt ggtttgggac gagttggccg gacgaacccg ggaagagtcc
     3061 acgctcaacg tcacgttctt gacgttcctc tgcctggcgt gcctgat
//
LOCUS       DASYLX010000671         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3874428,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000671 DASYLX010000000
VERSION     DASYLX010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3874428"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VGP27_15920"
     CDS             <1..825
                     /locus_tag="VGP27_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEV7852820.1"
                     /translation="MRYTAQGLGRVRKSSKKFSAELTRPFTPPPTFMNHKVDADRKFA
                     TATLALADVKETAKHLGVTINDMVLAISAGALRGLCLKYDGRADHPLLASVPVSIDFS
                     RDRVSGNYFSGVMMVVPIELDDPLERVQAVHDAAVDAKETHHLMGPELVSRWASYFPP
                     APAEALFRWLAEKDGQNKVLNLPISNVPGPREPGRVGGALVTEIYSVGPLTTGSGLNI
                     TVWSYVDQLNISVLSDGATLADPHELTDAMIEAFVEIRLAAGLSEDLTVIEKAMAP"
     gene            complement(826..2061)
                     /locus_tag="VGP27_15925"
     CDS             complement(826..2061)
                     /locus_tag="VGP27_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7852821.1"
                     /translation="MGKRRPLLRASVELANAANAVRPFGRDGYPTVPAFFAGWPTTEM
                     APLYMAGSMLGAVWRWLRGDYSTRGGRISLLLKAVSWGLLYLIHRRNVASEPYFETPL
                     RDTLGDDYEAVAAESQPIGRRRLSHMFPNEFLRRRYVEKTDIVRYGPHRANFADIWRR
                     RDLPRDGKAPVLLQVPGGAWSIGMRRPQAYPLMSRMAERGWICVSIGYRVSPVHTWPD
                     HIVDVKRALAWVKEHIAEYGGDPDFVAITGGSAGGHLCALAALTPDDPQYQPGFEDAD
                     TSVVAAVPIYGRYDWFTTKGPGRWEFVLIMLQRLVVKKRLTKHLQVYRDASPITRVRP
                     DAPPFFVLHGVDDSLIPVPEARAFVAAMQDVSKEAVVYSEIPHAQHAFDFFGSPRGYY
                     TAHAVEKFLSWVHARRDER"
     gene            complement(2054..>2831)
                     /locus_tag="VGP27_15930"
     CDS             complement(2054..>2831)
                     /locus_tag="VGP27_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7852822.1"
                     /translation="RATGLAVVPVMFDRIMELPDGVRSRYSGRSLRFAAASGSRMRPD
                     VVTAFMDQFGDVIYNNYNATEAGLIASATPEDLRAAPDTAGKPAPGTEIRVLDKDFRE
                     VPTGEVGQIFCLSGTLFDGYTTGKTKDFHEDFMASGDMGYVDANGRLFVVGRDDEMIV
                     SGGENVYPIEVEKTLASHADVAEAAVIGVDDEQYGQRLAAFVVLNDGAGATPERLKQH
                     VRESLANYKVPRQITVLDELPRSITGKIVRKDLHALVDNG"
BASE COUNT          478 a          941 c          928 g          484 t
ORIGIN      
        1 atgcggtaca ccgcccaggg cttggggcgc gtgcgcaaaa gctccaagaa gttctcggcc
       61 gagctcaccc ggccgttcac accgccaccg accttcatga accacaaggt cgatgcggac
      121 cgtaaattcg ccaccgccac actcgccctt gccgacgtca aggaaaccgc caaacatctc
      181 ggcgtcacga tcaacgacat ggtgctcgcg atctcggccg gggcgctacg cgggctgtgc
      241 cttaagtacg acggtcgggc cgaccatccg ttgttggcct ccgtcccggt gagtatcgac
      301 ttttcgcgag atcgcgtctc gggcaactac ttcagcggtg tgatgatggt tgtaccaatc
      361 gagctcgacg atccgctgga gcgggtgcag gcggtgcatg acgccgcggt cgacgccaag
      421 gagacccacc atctgatggg gcccgagctg gtcagccgat gggcctccta tttcccgccg
      481 gctccggcgg aggcgctatt ccgctggctc gcggagaagg acggtcaaaa caaggtgctc
      541 aacttgccca tctcgaacgt gccgggaccg cgtgagcccg gcagggtcgg tggcgcgctg
      601 gtgaccgaga tctactccgt ggggccgctg acgaccggca gcggtttgaa catcacggtg
      661 tggagctatg tcgaccagct caacatctcg gtgctgtccg acggtgcgac gctcgccgac
      721 ccgcatgaac tgaccgacgc aatgatcgag gccttcgtcg aaatccgcct cgccgcaggg
      781 ctttccgagg acctgacggt gatcgaaaag gcgatggcgc cctaactacc tctcgtcccg
      841 ccgcgcgtgc acccacgaca ggaacttctc gaccgcgtgc gcggtgtagt aaccgcgcgg
      901 cgagccgaag aagtcgaacg cgtgctgtgc gtgcgggatc tcggagtaga cgacggcctc
      961 cttcgagacg tcttgcatcg ccgccacgaa tgcgcgcgcc tcgggcaccg gaatcagcga
     1021 gtcgtcaacg ccgtgcagca cgaagaacgg cggggcgtcg ggtcgcaccc gggtgatcgg
     1081 cgacgcatcg cggtagacct gcagatgctt ggtcagccgc ttcttgacga ccaaccgctg
     1141 cagcatgatc aaaacgaact cccagcggcc cggccccttg gtggtgaacc agtcgtatcg
     1201 gccgtagatc ggaaccgctg cgacgacaga ggtgtcggcg tcctcgaaac cgggctgata
     1261 ctgcgggtcg tcgggcgtca gcgcggccag cgcacacaga tgcccgccgg ccgagccgcc
     1321 ggtgatcgcc acaaagtcgg ggtcgccgcc gtactcggcg atgtgctcct tgacccaggc
     1381 caacgcgcgc ttgacgtcga cgatgtggtc gggccaggtg tgcacggggc tcacccggta
     1441 gccgatcgag acgcagatcc atccgcgttc ggccatccgg ctcatcagcg gataggcctg
     1501 cggacggcgc atgcctatcg accacgcccc tccgggaacc tgcagcagca ccggggcctt
     1561 accatcacgt ggtaggtcgc ggcgacgcca gatgtcggcg aagttggctc ggtgtggccc
     1621 gtacctgacg atgtcggtct tctccacgta ccggcgacga aggaactcgt tcggaaacat
     1681 atgactgagg cgtcggcgcc cgatcggctg cgactccgcg gcgacggctt cgtagtcgtc
     1741 gccgagagtg tcccgcagcg gtgtctcgaa gtacggttcc gacgccacgt tgcggcgatg
     1801 gatgagatac agcaatcccc aggagaccgc cttgagtagc aaggagattc gtccgccacg
     1861 ggttgagtag tcgcctcgca gccatcgcca cacggcgccg agcatcgagc ccgccatgta
     1921 cagcggtgcc atctcggtgg tcggccaacc cgcgaagaac gcggggacgg tggggtagcc
     1981 gtcgcgaccg aacggccgca cggcgttggc ggcgttggcg agttcgacgc ttgcgcggag
     2041 cagcggcctg cgtttaccca ttgtcgacca gggcgtggag gtctttgcgc acgatcttgc
     2101 cggtgatgct gcgcgggagc tcgtcgagca ccgtgatctg ccgcggcact ttgtaattcg
     2161 ccaggctctc gcgcacgtgc tgcttcaggc gctccggcgt cgcgcccgcg ccgtcgttga
     2221 ggacgacgaa cgctgccagc cgctggccgt actgttcgtc gtccactccg atgacggcgg
     2281 cctcggccac gtcggcgtgc gacgccaggg tcttctcgac ctcgatgggg tagacgttct
     2341 cgccgccgga gacgatcatc tcgtcgtcgc ggccgacgac gaacagcctg ccgttggcgt
     2401 cgacgtagcc catgtcgccc gaggccatga agtcctcgtg gaaatccttc gtcttgcccg
     2461 tggtgtaccc gtcgaacagc gttccgctga ggcagaagat ctggccgacc tcaccggtcg
     2521 gcacctcgcg gaagtccttg tccagcactc ggatctccgt gccgggagcc ggcttgcccg
     2581 ccgtgtcggg agcggcccgc aggtcttcgg gcgtggccga cgcgatcaat ccggcctccg
     2641 ttgcgttgta gttgttgtag atgacgtcac cgaactggtc catgaaggct gtcaccacat
     2701 ccggccgcat ccgcgacccc gatgcggcgg cgaaccgcag cgaccgtccg ctgtagcggc
     2761 tgcgtacccc gtccggcagt tccataatcc ggtcgaacat caccggcacc acggcgagcc
     2821 cggtcgcgcg g
//
LOCUS       DASYLX010000672         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1132052,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000672 DASYLX010000000
VERSION     DASYLX010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132052"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGP27_15935"
     CDS             complement(<1..387)
                     /locus_tag="VGP27_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852823.1"
                     /translation="MSAPVPAPSRRPRIIEAVFWLWLLAAIALVLLGLLMALTRGNLP
                     TVYRGAGILFAVAGLALGYLAGRTRAGALRPRRAAVGLSLALVVLLALFTLLTGGILW
                     LIPMILTMVGAVLIMRPSAQDWFHTEE"
     gene            complement(399..800)
                     /locus_tag="VGP27_15940"
     CDS             complement(399..800)
                     /locus_tag="VGP27_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852824.1"
                     /translation="MSEARPADVDTGFWLWLVAVPLMVAGYVVDLVDTHRPHPPAVVF
                     VFSGMFVFVLAAVVVTFLVLMRHGYRWARTVLTGGGVASVVYSVTSLFSTDRPTTSAV
                     LYAVSAIVGSVLIIGGVFLLHRKDAHAFFVR"
     gene            complement(832..1812)
                     /gene="hemB"
                     /locus_tag="VGP27_15945"
     CDS             complement(832..1812)
                     /gene="hemB"
                     /locus_tag="VGP27_15945"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV7852825.1"
                     /translation="MPYPSHRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEP
                     KPIASMPGVVQHTRDSLRKAAADAVNAGVGGLMLFGVPREEDKDAVGSAGVAEDGILN
                     VALRDLAKDLGDETVLMADTCLDEFTDHGHCGVLDGSGRVDNDMTNARYIELAVAQAD
                     SGAHVVAPSGMMDGQVAAIRAGLDTAGHTDVVILAYAAKFASAFYGPFREAVSSSLSG
                     DRRTYQQDPGNAREALHEIELDIAEGADIVLVKPAMGYLDVVAAAADISPVPVAAYQI
                     SGEYSMISAAAANGWIDGRAAALESLTGIRRAGADIVLTYWAADVADWLA"
BASE COUNT          320 a          631 c          608 g          254 t
ORIGIN      
        1 ctcctcggtg tggaaccagt cctgtgcgga tggccgcatg atcagcacgg cccccaccat
       61 cgtcaggatc atcggtatca gccacaggat gccgcccgtc aggagtgtga acagcgcgag
      121 caggacaacg agtgcgagcg agagcccgac ggccgcccgc cgcggccgaa gcgcgcccgc
      181 cctggtgcgg ccggccagat agccgagggc caatcccgcc actgcgaaca ggatcccggc
      241 gcctcggtag accgtcggca ggttgccgcg cgtcagcgcc atcagcaggc cgagaagtac
      301 cagcgctatc gccgccagca gccagagcca gaacaccgct tcgatgatgc gtggccggcg
      361 tgacggcgcg ggcacggggg ctgacatggt cggcaagcct aacgaacgaa gaaggcatgg
      421 gcgtctttgc ggtgcagcag gaacactccg ccgatgatca gcaccgagcc gacgatggcg
      481 ctgaccgcgt agagcaccgc ggacgtggtc ggccggtcgg tggagaacag gctcgtaact
      541 gagtacacca ccgaggcgac cccgccgccg gtcagcaccg tgcgggccca gcgatagccg
      601 tgccgcatca ggaccaggaa cgtgaccacg accgctgcga gcacgaacac gaacatcccg
      661 gagaagacga agacgaccgc gggcgggtgc ggccggtggg tgtccaccag gtccaccacg
      721 tagccggcca ccatcaaggg caccgccacc agccacagcc agaagcccgt atcgacgtcg
      781 gcgggccgtg cctcgctcac gctgtgccga tggttcgctc gcagggttcg ctcacgccag
      841 ccagtcggcc acgtcggccg cccagtacgt cagcacgatg tcggcgcctg cgcgccggat
      901 gccggtcagc gactccagtg cggcggcccg gccgtcgatc cagccgttgg cggccgcggc
      961 gctgatcatc gagtactcgc cggagatctg gtaggcggcc accggcaccg gcgagatgtc
     1021 cgccgccgcc gccaccacgt cgagataacc catcgcgggt ttgaccagca cgatgtcggc
     1081 gccttctgcg atgtccagtt cgatttcgtg cagagcctcg cgagcgttgc ccggatcctg
     1141 ttggtaagta cggcgatccc cgctcaagct ggaggacacc gcctcacgga acggaccgta
     1201 gaacgccgac gcgaacttcg cggcgtacgc cagaatcacc acgtcggtgt ggccggccgt
     1261 atccaggccg gcccggatcg cggccacctg accgtccatc atcccgctgg gagcgacgac
     1321 gtgagcacct gaatctgcct gcgccacagc aagttcgatg tatcgggcat tcgtcatgtc
     1381 attgtcgacg cgtccggatc cgtcgagcac cccgcagtgg ccgtggtcgg tgaactcgtc
     1441 gaggcaggtg tcggccatca gcacggtctc gtcgccgagg tccttggcga ggtcgcgcag
     1501 cgcgacgttg aggatgccgt cctcggcgac accggcggag ccgacggcgt ccttgtcctc
     1561 ttcccgcggg acgccgaaca gcatcagccc gccgacgcct gcgttcaccg cgtcggccgc
     1621 cgccttgcgc agcgagtcgc gggtgtgctg caccaccccc ggcatggacg cgatcggttt
     1681 cggctcgtcg atgccgtcgg cgacgaacat cggcagcacc agatgccttg gctccaaaga
     1741 cgtctgcgcg accagtcgac gaagcgccgg tgtcgaccgg agtcgacgcg gacggtgact
     1801 cggatagggc atg
//
LOCUS       DASYLX010000673         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_363834,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000673 DASYLX010000000
VERSION     DASYLX010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363834"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..727
                     /gene="gpgP"
                     /locus_tag="VGP27_15950"
     CDS             59..727
                     /gene="gpgP"
                     /locus_tag="VGP27_15950"
                     /EC_number="3.1.3.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate phosphatase"
                     /protein_id="HEV7852826.1"
                     /translation="MTIRRLVMLRHGQTDFNAGNRMQGQLDTELSDLGRDQAVAAAEV
                     LAKRQPLLIVSSDLRRALDTATTLGERSGMPLQVDARLRETHLGDWQGMTHIEVDAAA
                     PGARIAWRNDARWAPHGGESRVDVAARGMPLVRELVSDQPEWGTDEPERPVVLVAHGG
                     LIAALTAALLKLPVDNWPALGGMGNASWVQLSGHSDDDEEFDDIRWRLDVWNASAQVA
                     NDVL"
     gene            717..1448
                     /gene="octT"
                     /locus_tag="VGP27_15955"
     CDS             717..1448
                     /gene="octT"
                     /locus_tag="VGP27_15955"
                     /EC_number="2.3.1.273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016414 - O-octanoyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosylglycerate octanoyltransferase"
                     /protein_id="HEV7852827.1"
                     /translation="MSSDRRTLLVFCDSLAYYGPTGGLPSDDPRIWPNIVAAHLDWDV
                     ELIGRIGWTCRDVWWAATQDPRSWAALPRAGAVIFATGGMDSLPSPLPTALRELIRYV
                     RPSWLRRWVRDGYGWAQPRLSPIARAALPPHVSVEYLEMTRHAIDFNRPGIPVVASLP
                     SVHIAETYGKAHHGRPGTVKAITAWAEQHNVPLVDLKAAVAEHITSGRGNPDGIHWNF
                     EAHEAVAELMVKGLQEAGVVQEDAP"
     gene            1451..>2208
                     /locus_tag="VGP27_15960"
     CDS             1451..>2208
                     /locus_tag="VGP27_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV7852828.1"
                     /translation="MAVVVVTDSSSRLQAEDMQRWGIRQVPLHILLDGNDFRDGIDDV
                     PADAHQRHATTAGATPAELADTYRAALQQSDGDGVVAVHISAALSSTYSSAVSAAREF
                     GPAVRVVNSRSAAMGVGFVALAAAERAASGADLDAVETAARSAVPRGHSFIVVHRLDN
                     LRRSGRIPRGASLLGSALSLKPLLCLDVDGRLVLSQRIRTATKAHAALIDQIAEVVGD
                     TGARIAVHHVDNRDAADDIAKSLTVRLPQIAPPTV"
BASE COUNT          346 a          738 c          766 g          358 t
ORIGIN      
        1 cgctcgaccg gctttggcgg gactgcccgg tcgttccggt ggatctcgag gcggggccgt
       61 gacgatccgc cgcctggtga tgctgcggca cggacaaacc gacttcaacg cgggcaaccg
      121 catgcagggt cagctcgaca ccgagttgag cgacctgggc cgcgaccagg ccgtcgccgc
      181 ggccgaggtg ctggccaaac ggcagccgct gctgatcgtc tcctccgacc tgcgtcgggc
      241 gctcgacacc gcgacgaccc tgggggagcg atcgggaatg ccgctgcagg tggacgcccg
      301 gctgcgtgaa acgcacctcg gggactggca gggcatgacg cacatcgagg tggatgccgc
      361 cgcacccggc gcgcggatcg cgtggcgcaa cgacgccagg tgggcaccgc acggcggtga
      421 gagccgtgtc gatgtggccg cgcgcggcat gccgctggtc cgcgaattgg tttctgatca
      481 acccgagtgg gggacagacg aaccggagcg gccggtggtg ttggtggcac acggggggct
      541 gatcgccgcg ctgacggcgg cgctgctgaa gctgccggtg gacaactggc ccgcgctggg
      601 cggcatgggc aacgccagct gggtgcaact ctccggacac tccgacgacg acgaagaatt
      661 cgacgatatc cggtggcgcc tcgatgtctg gaacgcctcg gcgcaggtcg ccaacgatgt
      721 cctctgaccg gcggaccctt ctcgtcttct gcgactcgct tgcctactac gggccgaccg
      781 gcggcctgcc atccgacgat ccgcgaatct ggcccaacat cgttgctgct catctcgact
      841 gggatgtcga gctgatcgga cggatcggct ggacgtgccg ggacgtgtgg tgggcggcga
      901 cgcaggaccc gcgctcgtgg gcggcgctgc cccgagccgg ggccgtcatc ttcgcgaccg
      961 gggggatgga ttcgcttccg tcgccgctgc cgacggcgtt gcgtgaactc atccgctatg
     1021 tgcggccatc gtggctgcga cgctgggtac gcgatggtta cggctgggcg cagcctaggc
     1081 tctcgccgat cgcgcgggcc gcattgccac cgcacgtctc cgtcgagtac ctcgagatga
     1141 cccgtcacgc aatcgatttc aaccggccgg gtattcccgt cgtggcgtcg ctgccgtcgg
     1201 tgcatattgc cgagacgtac ggcaaggcgc accacggccg accgggcacc gtcaaggcga
     1261 tcactgcatg ggcggaacag cacaacgttc cgttggtcga cctgaaagcc gccgtcgccg
     1321 agcacatcac gagcggcaga ggcaatcctg acggcatcca ctggaacttc gaggcgcatg
     1381 aagcggttgc cgaacttatg gtcaagggcc tgcaggaagc cggagttgtt caagaggatg
     1441 caccctgatc atggccgttg tcgtggtgac cgactcgtct tcgcgcctgc aggccgagga
     1501 catgcagcgc tggggtatcc gacaggtacc gctgcacatc ttgttggacg gcaacgattt
     1561 tcgcgacggc attgacgacg tgcccgccga cgctcaccag cgccacgcca ccaccgcggg
     1621 cgcgacgccc gccgaactcg ccgacacata tcgcgcagcg ctacagcaaa gcgacggcga
     1681 cggggtggtg gccgtgcaca tctcggccgc gctctcgagc acctacagtt ccgcggtgtc
     1741 ggcggcgcgc gagttcggtc ccgccgttcg cgtcgtgaac tccaggtcgg ccgccatggg
     1801 cgtcgggttc gtcgcgctgg ccgccgccga acgtgccgca tcgggggcag acctcgatgc
     1861 cgttgagact gctgctcgtt cggccgttcc tcgcggccat tcgttcatcg tcgttcaccg
     1921 gctggacaac ctgcggcgca gcggcagaat ccccaggggg gcatccctat tgggatccgc
     1981 gttgtcgctg aagccgctcc tgtgccttga cgtcgacggc aggttggtgc tgagccagcg
     2041 gatccgcacc gccaccaagg cgcatgcggc gttgatcgat cagatcgccg aggtggtcgg
     2101 cgacaccggc gccaggatcg ccgtacatca cgtcgacaac cgcgacgccg cggacgacat
     2161 cgccaagtcg ctgaccgtgc ggctgccgca gatcgcgccg ccgaccgt
//
LOCUS       DASYLX010000674         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_803927,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000674 DASYLX010000000
VERSION     DASYLX010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803927"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VGP27_15965"
     CDS             complement(<1..858)
                     /locus_tag="VGP27_15965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV7852829.1"
                     /translation="MADQPIDLSEEALAKVVSAAEHAFDLAADLDTLARAKTEHLGDR
                     SPLALARQALGSLPKADRADAGKRVNIVRAKAQRSYDERLAALRAERDAAVLVAESID
                     VTLPSTRQPIGARHPITILAEHVADTFVAMGWELVEGPEVETEQFNFDALNFPPDHPA
                     RSEQDTFYIAPDGSRQVLRTHTSPVQIRTLLERELPVYIISVGRTFRTDELDSTHTPV
                     FHQVEGLAVDKGLTMAHLRGTLDAFARSEFGPHARIRFRPHFFPFTEPSAEVDVWFEN
                     KKGGPGFVEW"
     gene            complement(910..2343)
                     /locus_tag="VGP27_15970"
     CDS             complement(910..2343)
                     /locus_tag="VGP27_15970"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7852830.1"
                     /translation="MDDNRGARGLSRRDFIGATAAAASGLALSECGWTRETSGELPNP
                     EDSGIDHIIVLMMENRSFDHFLGWVPGADGMQAGTRLVDAEGTAHESYDLVPNFQNCY
                     FADPDHSYAGGRREINGGAMDGFLLTQPPGDTFPVGYYTADSLPFFKGCAEHWTICDR
                     YFSGILSSTTPNRIYMHAGQTDRLANTVDISALPTVWDRMLQIGRSVAYYYVDVPYTA
                     FWGDKYQAFSKNYSLQELADDIAAGRLANLTFVDNVGTEFEEMFGLSRDDHPLADIRD
                     GQAFLNEIYDAVRTGPQWERSLLAIVYDEWGGFYDHVAPPFAPVSAAEAALGNDGRLG
                     CRVPCVLIGPRARRGVVEHTQFDPNSILNLLSWRFGFEPLGVRGNSTNLALALDWGSR
                     PTVDAPAFDVPPGPFGGLCMPLPTLAQLAGIPMPNPPPDIDPVPLAQQIQLPPVPGLD
                     IPEPLRRLVDHERELDALRDLARRFGL"
     gene            complement(2303..3049)
                     /locus_tag="VGP27_15975"
     CDS             complement(2303..3049)
                     /locus_tag="VGP27_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HEV7852831.1"
                     /translation="MTTALAVLGPQVQNRVITHASTNLHNLSHGHLGTLFVSAFVIDA
                     GPIYIWLPGLVCLLAVAELLWRSGRLVVAFAVGHVGATLVVAVGLTAAVELGWLSADV
                     TRATDVGMSYGASAVLGALTAAIPRRWRPAWTGWWLAVAVSVVVLGGGFTDVGHAMAL
                     VSGMVISSRFGRPAAWTPVRYLLLVVASAFGFLMLANTGLSLVMATGLGAMGALVFEI
                     GAGILSPRGAGAAELPQHGRQPGRARAESA"
BASE COUNT          553 a          990 c         1033 g          474 t
ORIGIN      
        1 ccattccacg aagcccgggc cgcccttctt gttctcgaac cacacgtcga cctcggcgga
       61 cggttcggtg aacgggaaga agtgcggccg gaaccgaatg cgggcgtgcg gcccgaactc
      121 cgagcgggcg aaggcgtcca gggtgccacg caagtgggcc atcgtcagcc ccttgtccac
      181 tgccagaccc tcgacctgat ggaagaccgg ggtgtgggtg gagtcgagct cgtcggtgcg
      241 gaaggtccgg ccgaccgaga tgatgtaaac cggcagttcg cgttcgagca gcgtgcggat
      301 ctgcaccggc gaggtgtggg tgcgaagcac ctgccgcgat ccgtccggcg cgatgtagaa
      361 cgtgtcctgc tcgctgcggg cgggatggtc cggcgggaag ttgagcgcgt cgaagttgaa
      421 ctgctcggtt tcgacctccg gtccctccac aagctcccaa cccatggcga cgaacgtgtc
      481 ggcgacgtgc tcggcgagga ttgtgatcgg gtgacgggcg ccgatcggct gacgcgtcga
      541 gggcagtgtc acatcgatgc tctcggcgac cagcaccgcg gcgtcgcgtt cggcgcgcag
      601 cgccgcgagg cgttcgtcat agctgcgctg cgccttggca cggacgatgt tcacccgctt
      661 gcccgcgtcg gctcgatcgg ccttcggcag agaccccagc gcctgccgcg ccagcgccag
      721 cggtgagcgg tcgcccaggt gctcggtctt cgcccgcgca agggtgtcga ggtcggcggc
      781 gaggtcgaag gcatgctcgg ccgcgctgac caccttggcc agcgcttcct ccgacaaatc
      841 tatgggctga tcagccaccc ggcgatcata ggtgatcgac agatgagagc gacgtcacgc
      901 gtgattcgac tagaggccga accggcgtgc aaggtctcgc agcgcatcca attcccgttc
      961 gtggtcaacc agacggcgca gcggctccgg aatgtccagg cccggcaccg gaggcagctg
     1021 aatctgctgc gccagcggca cagggtcgat gtcgggcggc gggttcggca tcgggatgcc
     1081 tgcgagttgg gccagtgtcg gcagcggcat gcagaggccg ccgaaaggac cgggcggcac
     1141 atcgaatgca ggcgcgtcca ccgtcggccg cgatccccag tcgagcgcga gggccagatt
     1201 ggtcgagtta ccacgcactc ccagcggttc gaagccgaag cgccaggaga gcaggttcag
     1261 gatcgagttc ggatcgaatt gtgtgtgctc gacgacgccg cgccgcgcac gcggaccgat
     1321 cagcacacac ggaacccggc agccgaggcg gccgtcgttg ccgagcgccg cctcggcggc
     1381 ggagacgggg gcgaacggag gcgccacatg gtcgtagaaa ccgccccatt cgtcgtagac
     1441 gatggcgaga agcgagcgct cccactgggg cccggtgcgc accgcgtcat agatctcgtt
     1501 caggaacgcc tggccgtcgc ggatgtcggc gagaggatga tcgtcgcggg acaacccgaa
     1561 catctcctcg aactcggttc ccacgttgtc gacgaaagtc aggtttgcga gtctccccgc
     1621 ggcgatgtcg tcggccagct cttgcaagga gtaattcttc gaaaacgcct ggtacttgtc
     1681 gccccaaaac gccgtgtacg gcacgtcgac gtagtagtag gcaacgctgc ggccgatctg
     1741 caacatgcgg tcccagaccg tcggcaacgc agatatatcc acggtattcg ctaaccggtc
     1801 ggtctggccg gcatgcatgt agatgcggtt gggcgtcgtt gacgagagga tgccgctgaa
     1861 gtagcggtcg cagatggtcc aatgctcggc gcagccctta aagaacggca ggctgtcggc
     1921 ggtgtagtag ccgaccggaa aggtgtcccc tggcggctgc gtcagcagga agccatccat
     1981 cgccccgccg ttgatctcgc ggcgcccccc tgcatagctg tggtctggat cggcgaaata
     2041 gcagttctgg aaattcggga caagatcgta ggattcgtgc gctgtgcctt ccgcatcgac
     2101 aagtcgggtc ccggcctgca tgccgtcggc gccggggacc cagcccaaga agtggtcgaa
     2161 cgagcggttc tccatcatca ggacgatgat gtggtcgatg ccggagtcct ccgggttggg
     2221 gagctcgccc gaagtttctc tggtccatcc gcattccgac agtgccagac cacttgcggc
     2281 ggcggcggtg gcaccgatga agtcacgccg actcagcccg cgcgcgcccc ggttgtcgtc
     2341 catgctgtgg taattctgca gccccggcgc cgcggggact gaggattccg gccccaatct
     2401 cgaacacgag tgcgcccatg gcgccaagtc cggtcgccat gaccaacgac agcccggtgt
     2461 tggccagcat cagaaatccg aaggccgaag cgacgaccag cagcagatac cgcaccggcg
     2521 tccaagctgc gggccgaccg aagcggctcg agatgaccat gccgctcacc agcgccatcg
     2581 catgaccgac gtcggtgaaa cccccgccca gaaccaccac cgacaccgcg accgccagcc
     2641 accagcccgt ccacgccggt cgccatcgcc gcggaatcgc cgcggtgagc gcccccaaaa
     2701 cggcggaggc accgtagctc atgccgacgt cggtggcccg agtcacgtct gccgacagcc
     2761 agcccagttc gacggcggcc gtgagaccaa cggcgaccac aagcgtggcg ccgacgtggc
     2821 cgaccgcgaa cgccaccacc aggcggccgc tgcgccacag cagttcggcg accgcaagca
     2881 ggcacaccaa gccgggtagc cagatataaa tcggcccggc gtcgatgacg aacgcgctga
     2941 cgaacagcgt gccgaggtga ccgtggctga ggttgtgcag atttgtgctg gcgtgggtga
     3001 tgacgcggtt ctgcacctgg ggtccgagga ccgccaaagc ggttgtcaca
//
LOCUS       DASYLX010000675         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1573049,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000675 DASYLX010000000
VERSION     DASYLX010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573049"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGP27_15980"
     CDS             <1..270
                     /locus_tag="VGP27_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEV7852832.1"
                     /translation="SRVAGSARLELDLRLVDFFGTAGFAALHNINVICSRYEATWVLR
                     AGRQVRRLLAICDPDGSLPLEEPRSVLDDVHTSARDCELLVGRNN"
     gene            complement(205..1353)
                     /locus_tag="VGP27_15985"
     CDS             complement(205..1353)
                     /locus_tag="VGP27_15985"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV7852833.1"
                     /translation="MADKPTVLVINSGSSSLKYAVVQPDSGTVVVDGIVERIGDGPVA
                     DHGAALQEAFKRLAESGRPLESLGLLAVGHRIVHGGPDLYKPTLVDEALIAKVDALAP
                     LAPLHNPPALLGIQVARDAMPDLPHVAVFDTAFFHDLPAAAATYAIDRDIARQWQIRR
                     YGFHGTSHQFVSEQVATFLDEPLGSLNQIVLHLGNGASASAISGGRPVDTSMGLTPLE
                     GLVMGTRSGDIDPGIISYLSRTAGMRVEDIETMLNKRSGVRGLGGEIDFRVLHQRIES
                     GDAAAQLAYDVYIHRLRKYIGAYMAVLGNTDVITFTAGVGENDAVVRRDALAGLTTLG
                     IELDGRLNESSDKCARRISAETSRTTVLVVPTDEELAIARACVDVVED"
     gene            complement(1353..>1971)
                     /gene="pta"
                     /locus_tag="VGP27_15990"
     CDS             complement(1353..>1971)
                     /gene="pta"
                     /locus_tag="VGP27_15990"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV7852834.1"
                     /translation="MVSGAMHTTAHTVRPAFEIIKTEPGITTVSSIFLMCLADRVLAY
                     GDCAIVPDPTSEQLADIAISSARTAAQFGIDPRVAMLSYSTGTSGTGADVDKVRAATD
                     LVRERQPDLLVDGPIQYDAAVDPTTAVSKMPDSKVAGRATVLIFPDLNTGNNTYKAVQ
                     RSAGAIAIGPVLQGLNKPVNDLSRGALIEDIVNTVAITAIQAQDQ"
BASE COUNT          318 a          645 c          664 g          344 t
ORIGIN      
        1 agcagggtcg ccggatcggc gcgcctcgag ctcgacctgc ggctcgtcga cttcttcggc
       61 accgccggat tcgccgcgct gcacaacatc aacgtcatct gcagccgcta cgaagcgacg
      121 tgggtgctgc gggcgggccg gcaagtccgc cggttgttgg ccatctgcga tccggacggc
      181 agcctgccgc tcgaagagcc gcggtcagtc ctcgacgacg tccacacaag cgcgcgcgat
      241 tgcgagctcc tcgtcggtcg gaacaactag aaccgtcgtc cgcgacgtct ccgcggagat
      301 tcggcgtgcg catttgtcgg agctttcgtt gaggcgcccg tcgagttcga ttcccagcgt
      361 cgtcagcccg gccaacgcgt cgcgacggac gaccgcgtcg ttctcgccga cgcccgcggt
      421 gaaggtgatg acgtcagtgt tgccgagcac cgccatgtag gcaccgatgt acttgcgcag
      481 ccggtggatg tacacgtcgt acgccaactg tgctgccgca tcaccggatt cgatgcgctg
      541 gtgcagcacg cggaagtcga tctcgccgcc gagaccgcgg actccggaac gcttgttgag
      601 catcgtctcg atgtcctcga cacgcatccc tgcagtgcgc gacagatacg agatgatccc
      661 ggggtcgata tcgcccgacc gggtgcccat caccagaccc tccaacggcg tgaggcccat
      721 cgacgtgtcg acgggcctgc cccccgagat cgccgacgcc gatgcgccgt tgccgaggtg
      781 caggacaatc tggttcagcg aacccagcgg ttcgtccagg aaggtcgcca cctgttcact
      841 cacgaattgg tgcgaggtgc cgtggaaccc gtagcgccga atctgccact gccgcgcgat
      901 atcccggtcg atcgcgtatg tcgcggcggc ggcgggcagg tcgtggaaga aggccgtgtc
      961 gaaaacggcg acatgcggca ggtcgggcat ggcgtccctg gccacctgga tgccgagcag
     1021 cgcaggcggg ttgtgcagag gagccagggg tgcgagcgcg tcgaccttgg cgatgagcgc
     1081 ctcgtcgacc agtgtcggct tgtacaggtc aggaccgccg tgcacgatgc gatggcccac
     1141 cgccagcagg ccaaggcttt ccagcggtcg cccggattcc gcgaggcgct tgaaggcctc
     1201 ctgcagggcc gcgccgtggt ccgccaccgg gccgtcaccg attcgctcca cgatcccgtc
     1261 gaccacgacc gttcccgagt ccggctgtac aacagcatat ttgagtgacg acgagccgga
     1321 gttgatgacg agcacggtgg gcttgtcggc catcactggt cctgtgcctg aatggcggtg
     1381 atcgccacgg tgttgacgat gtcctcgata agcgcgcccc gcgacaggtc gttgaccggt
     1441 ttattgaggc cctgcagcac cgggccgatg gcgatggcgc ccgcgctgcg ctgcacggcc
     1501 ttgtaggtgt tgttgccggt gttgaggtcc gggaagatca gcaccgtcgc cctgcccgcg
     1561 accttcgaat cgggcatctt gctgaccgcc gtggtcggat cgacggccgc gtcgtactgg
     1621 atcggaccgt ccaccagaag gtcgggctgc ctctcacgca ccaaatcggt tgccgccctg
     1681 actttgtcga cgtcagcgcc ggttcccgag gtccccgtcg agtaggacag catggccacc
     1741 cgaggatcga tgccgaactg cgcggcggtg cgcgccgacg agatcgcgat gtcggccagt
     1801 tgctccgacg tcgggtccgg cacgatcgca cagtcgccgt aggcgagcac ccggtcggcc
     1861 aggcacatca ggaagatgct ggacaccgtg gtgatgccgg gttcggtctt gatgatctcg
     1921 aacgcgggcc ggacggtatg tgccgtggtg tgcatcgcgc cggagaccat t
//
LOCUS       DASYLX010000676         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3655835,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000676 DASYLX010000000
VERSION     DASYLX010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655835"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGP27_15995"
     CDS             <1..219
                     /locus_tag="VGP27_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HEV7852835.1"
                     /translation="AGAAVRWMRPFVPPIVASAIDTTTRPLRAARQVFEETEEIHVSI
                     RRSHRMSVTSEETADSDGQWRQLPSADA"
     gene            complement(232..846)
                     /locus_tag="VGP27_16000"
     CDS             complement(232..846)
                     /locus_tag="VGP27_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEV7852836.1"
                     /translation="MPELSAGYLGPLPDARPPGLRRRSVPAGTEIWRLDAQAPAKWTW
                     SGFPQPRYRFDPESGAFRTRYAGATLVGAFRERYRFSGLVIPADHATHRLVRLVAARD
                     LRVLDLRTEKNLDALQVDDQINTGQHPDVWHTCHRLADAVQRWWTDIDAIAYRSRTTP
                     ETSGNFAFFAIDGFDVESRALKGRADVLTDLVLNHDFTINWDID"
     gene            complement(839..1354)
                     /locus_tag="VGP27_16005"
     CDS             complement(839..1354)
                     /locus_tag="VGP27_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852837.1"
                     /translation="MTAAVAPLARQVAARAQRDKGFAQILDALLAAPGAPQGTLERIA
                     ARELNTQRRGALVQEFVDGSMRTSDVQALLGVKSPQAVHRLRTRKKLLGTAVGNQTWF
                     PAWQFHDDRVRTDLPRIIELLARFTDDVLIADRVMRTKHDDLGGKSIAEALRNTRTAE
                     TAWHVLASIGA"
     gene            complement(1412..1969)
                     /locus_tag="VGP27_16010"
     CDS             complement(1412..1969)
                     /locus_tag="VGP27_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family transcriptional regulator"
                     /protein_id="HEV7852838.1"
                     /translation="MLDSLWTAATRHPFLDAVRDGTISGSAFDRWLVQDALFVTDLLT
                     FQARLLARAPRPAQSVLVGGCAALVAELDWFEEQAARRRLDLSAPPLSATLAYRGLLQ
                     RLDAAPYEAAMTALWVVERVYLLAWSAAASDSSPFGEFVEHWSAPAFADYVGALGELA
                     VPDNHEALVVDVLSHEVAFWDMALV"
     gene            3006..>3200
                     /locus_tag="VGP27_16015"
     CDS             3006..>3200
                     /locus_tag="VGP27_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852839.1"
                     /translation="MSDPDTTQLWRMIVETRQLEDGTWRASIPARDWSERGDSEQEAR
                     DRAVEHAITDGEDADEIVRNA"
BASE COUNT          590 a          977 c         1109 g          524 t
ORIGIN      
        1 gcgggggcgg cggtgcggtg gatgcgtccg ttcgtgccgc cgatcgtcgc cagcgccatc
       61 gatacgacga cgaggccatt gcgggccgcg cgccaggtgt tcgaggagac agaggagatc
      121 cacgtcagca tcaggcgaag ccaccggatg agcgtgacct ccgaggaaac cgccgactcg
      181 gacgggcagt ggcgtcagtt gccctcggcc gacgcttagg ggtgatgtca cctagtcgat
      241 gtcccagttg atggtgaagt cgtggttcag cacgaggtcg gtcagcacgt cagcacggcc
      301 cttcagcgcg cgggactcga cgtcgaaccc gtcgatcgcg aagaacgcga agttgcccga
      361 cgtctcgggc gtcgtccgcg agcggtaggc gatcgcgtcg atgtcggtcc accagcgctg
      421 gaccgcatcg gccagccgat ggcaggtgtg ccacacgtcg ggatgctggc cggtgttgat
      481 ctggtcgtcg acctgcaggg cgtcgaggtt cttttcggtg cgcaggtcca ggacacgcag
      541 gtcgcgggcg gcgaccagcc gaaccaggcg gtgagttgcg tgatctgcgg gaatgacgag
      601 gccgctgaat cggtagcgct ccctgaatgc gcccacgagc gtcgcgcctg cgtagcgagt
      661 gcgaaaggcg cccgattcgg ggtcgaagcg atagcggggt tgcggaaagc cggaccaggt
      721 ccacttggcc ggagcttgcg cgtcgagtcg ccagatctcg gtgcctgcag gcactgaacg
      781 gcgtcgaagt ccgggtggcc tggcgtcggg gagtgggccg agataaccgg ccgagagctc
      841 aggcaccgat ggacgcgagt acgtgccacg ccgtttcggc ggtcctggtg ttgcgcagcg
      901 cttctgcgat cgactttccc ccgagatcgt cgtgcttggt acgcatgacg cggtcggcga
      961 tcagtacgtc gtcggtgaac cgggcgagta actcgattat cctcgggaga tccgtccgga
     1021 cgcggtcatc gtggaattgc catgcaggaa accatgtttg attgccaacg gcggtgccga
     1081 gcagcttttt ccgcgttcgg agccgatgga cggcttgagg cgacttcacg ccgagcagcg
     1141 cctgtacgtc ggacgtgcgc atcgagccgt cgacgaactc ttggaccagc gcgccacgcc
     1201 gctgcgtgtt cagttcgcgg gcggcgatgc gttcgagcgt gccctgtggg gcgcccggcg
     1261 cggccagcag cgcgtcaagg atttgggcga atcctttgtc tcgttgagcc cgggcagcga
     1321 cttgacgggc aagcggcgca acggcggctg tcatgttgtc agtctacgcc ggccactcgc
     1381 tattgaaaca atcgaaacaa gggtgacggc gtcataccag cgccatgtcc cagaacgcca
     1441 cctcatgcga cagcacgtcc acgacgagcg cctcatggtt atccggaacg gccagctccc
     1501 cgagcgcgcc gacgtagtcg gcgaaggccg gtgcggacca gtgctccacg aactcgccga
     1561 agggggacga gtccgacgcg gcggcgctcc acgcgagcag gtacacccgt tcaaccaccc
     1621 acagcgccgt catcgcggct tcgtagggcg ccgcgtccag tcgctgaagc agtccgcggt
     1681 aggcgagcgt cgccgacagt ggcggcgcgc tgagatcgag ccgacgacga gcggcctgct
     1741 cttcgaacca gtcgagttcc gcaaccaacg ccgcacatcc gccgacgagc acggactgag
     1801 ccggccgcgg cgcccgggca agcaggcggg cctgaaaggt cagcaggtcg gtcacgaaca
     1861 aggcgtcctg cacgagccac cggtcgaacg ccgagccgga aatcgtgccg tcgcgcaccg
     1921 cgtcgagaaa cggatgccgg gtggcggcgg tccacagtga atcgagcaca gtacgaaggc
     1981 tagtcccgcc ggcccgagcg gccgcccacc gaagcactgt cgcagttgcc cgcctcagag
     2041 cgacattgtc gctaaccgga taggcattca atgctttcag cgacaaagtc gctcgaaggc
     2101 gggcagaacg accgccgtcc ctttcgacaa ggtgaagaaa aatctccgcg aagaggagtc
     2161 ctgcaatggg cttatggtcg agatgtggaa acgattcggg ctgatgtcga cggccttttc
     2221 gcgttcggcg ccgcttgcaa gcgatcggca gaagcgttga gcgctgggca tcctgctcag
     2281 cagagcggcc caagttttca atcgtcgacc gcggctgtag aagctgtact gacgctggcg
     2341 ggtcgagccg aaaacctgat cagtatgcgg ctgcgcgtga ctggacatgc cgtcgtcagt
     2401 gccgggaacc ggctggccga tgcgacagcg cttccagcga gcgcatcgcc accgtcggcg
     2461 acgcgccaac agtgttgtga cgatggcagc ttcgaccgct ttgcccaggc tcagccagat
     2521 ccacggctgg gacgttagcc acctcgaagc ggccgcggat gcgtgggctg tacgcgcagc
     2581 agcgtgggag ggctgctatt ccgccggagc gccggattcg acgtcgaaga ggccctatcg
     2641 atcgtcgagg tcacgacgcc gtccccagcg acacgacatc aacgagaagc attggcggtg
     2701 acgcacgcat ggcgcgtctg gaactcggcc gacacgctgg cggccaccga tagagaggcg
     2761 gcaaggaaaa ttacccagac cgtagaaggt ttaggggatt tgaggtttcc gacagacccc
     2821 gatctacgat ggccgggcgc gatgatggct gattgcgccg gtgtagtcgg cccgcttcca
     2881 ggggcgccgc acctgatttc tgttacccga gtgctcgtcc cgatttctgg tggtgtgaag
     2941 gctatgacat cggtggcggt ccctatggat tcgattcccc catcgacgtg tcaggggtcg
     3001 gctgaatgag cgatcccgac acaactcagc tgtggcgaat gattgtcgag actcgacagc
     3061 tggaggacgg cacgtggcgc gcctccatcc cggcacggga ctggtcggag cgcggcgaca
     3121 gcgagcaaga ggcgagggac cgagccgtcg agcatgcgat caccgatggt gaggacgccg
     3181 acgaaatagt gcgcaatgcc
//
LOCUS       DASYLX010000677         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1022781,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000677 DASYLX010000000
VERSION     DASYLX010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022781"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VGP27_16020"
     CDS             <1..1026
                     /locus_tag="VGP27_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEV7852840.1"
                     /translation="YIVSEAMHALGIPTTRSLSVVATGRPVQRDTMLDGAVLARVAAS
                     HLRVGSFQYVAAAGELDVLRRLADHAIARHYPAAANADNPYLALLDAVITAQAELIAR
                     WMLVGFIHGVMNTDNMTISGETIDYGPCAFMDAFDPKTVFSSIDSWGRYAYGNQPTVA
                     GWNLARFAEALLPLFAEDHDRGIALATALLQDFPSRYASAWTAGIRAKLGLTEDVDDD
                     VVGQLADDLLALLERDRVDYTSFFRQLSGAARGEADSPFDEWVARWRALDPDADAMDR
                     VNPVYIPRNHLVEEALGAATAGDLVPLQTLLSAITSPYTERPGFERYAEPAPEDFGGC
                     YQTFCGT"
     gene            complement(1039..1941)
                     /locus_tag="VGP27_16025"
     CDS             complement(1039..1941)
                     /locus_tag="VGP27_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEV7852841.1"
                     /translation="MEAILEEFDDHLALERGRSDHTRRAYLGDLRSLFDFLSQRTPDA
                     QLSSLSLPALRSWLSAQAAAGVARTTLARRTSAVKTFTAWATRRGLLSSDPAARLQVP
                     KSRRTLPSVLRQDQALDAMAAANSGAEQGDPMALRDRLIVEMLYATGVRVSELCGLDV
                     DDVDMSRRLCRVLGKGNKQRTVPFGEPAQAALTAWLTDGRPALATGDSGPALLLGARG
                     RRLDQRQARTVVHQTVSAVEGAPDIGPHGLRHSAATHLLEGGADLRIVQELLGHSTLA
                     TTQLYTHVTVARLRAVHDQAHPRA"
     gene            complement(1973..3133)
                     /locus_tag="VGP27_16030"
     CDS             complement(1973..3133)
                     /locus_tag="VGP27_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HEV7852842.1"
                     /translation="MAFGDYQLEIYLQGLSGIVPNLPMAFADWEAKAQAAMPPSVWSY
                     VTGGAGDERTQRVNRTAFDNWGLMPRMFVGAKERDLSVDLFGLKLPSPVFMAPIGVIG
                     ICSQNGHGDVATARAAARTGVPMVISTLTEDPMENIAAEFGDTPGFFQLYTPANRDLA
                     ASFVQRAEAAGYKGIIVTLDTWVPGWRPRDLSTSNFPQLRGKCLSNYTSDPVFRAGLS
                     QPPEENPQAAVLSWVQTFGNPLTWEDLPWLRSLTKLPLLVKGICHPDDARRAIDGGVD
                     GIYCSNHGGRQANGGIPAIDCLPAVVEAANGVPVLFDSGIRNGADIVKALALGATAVG
                     IGRPYAYGLALGGTDGVVHVLRCLLAEADLIMAVDGYPTRKDLTPETLRRVT"
BASE COUNT          485 a         1095 c         1122 g          500 t
ORIGIN      
        1 tacatcgtca gcgaggcgat gcacgccctc ggcatcccga ccacacggtc gctctcggtc
       61 gtcgccaccg gccgcccggt tcagcgcgac accatgctcg atggcgccgt cctggcgcgg
      121 gtcgcggcga gccacctacg ggtcggcagc ttccaatacg tcgcggccgc aggcgaattg
      181 gatgtcctgc ggcgcctcgc tgatcacgcc atcgccaggc actaccccgc ggccgccaac
      241 gccgacaatc cctaccttgc cctgctcgac gcggtcatca ccgctcaggc cgagctcatc
      301 gcccgctgga tgctggtcgg gttcatccac ggcgtgatga acaccgacaa catgacgatc
      361 tcgggcgaga cgatcgacta cggtccgtgc gccttcatgg acgccttcga tcccaagacg
      421 gtgttcagct cgatcgactc ctggggccgc tacgcctacg gcaaccagcc gacggtggcc
      481 gggtggaacc tggcccgctt cgccgaggcg cttctgccgc tgttcgccga ggaccatgat
      541 cgcggaatcg cgctcgcgac ggcactgctg caggacttcc cgtcgcgcta cgcgtccgcg
      601 tggacggccg gcatccgcgc caagctcggg ctgaccgagg atgtcgacga cgacgtcgtg
      661 gggcagctcg ccgacgacct gctggcgctc ctcgagcgcg accgcgtgga ttacacatcc
      721 ttcttccgac agctgagcgg cgcagcgcgt ggagaggccg attcaccctt cgatgagtgg
      781 gtggcccggt ggcgggcgtt ggacccagac gccgacgcca tggatcgggt caacccggtc
      841 tacatcccgc gcaatcacct cgtcgaggag gcgctcggcg cggcgacggc cggcgatctc
      901 gttccgctgc agacgctgct gtcagccata acgtctccgt atacggagcg gccggggttc
      961 gagcgctatg ccgagccggc gccggaagac ttcggcggct gctaccaaac cttctgcggc
     1021 acttagcgat tcgctggttc aggcgcgtgg atgcgcctga tcgtgcaccg cacgcagccg
     1081 cgcgactgtg acgtgggtgt agagctgcgt ggtggccagc gtcgagtggc cgagaagttc
     1141 ctgcacgatg cgcagatccg cgccgccctc gaggagatgg gtggccgcgc tgtgccgcag
     1201 cccgtgcggt ccgatgtcgg gcgctccctc gacggcggag acggtctggt gcacgacagt
     1261 gcgggcctgg cgctggtcga gccgacggcc gcgggcgccg agcagcaacg cgggccccga
     1321 gtccccggtg gccagcgccg ggcgtccgtc ggtcagccac gccgtcaacg cggcctgggc
     1381 cggctcgccg aacggcaccg tgcgctgttt gttgccctta cccaggacgc ggcacagccg
     1441 tcgcgacatg tcgacgtcat cgacgtcgag gccgcacagc tcgctgacgc ggacgccggt
     1501 ggcgtagagc atctcgacga tgagccggtc ccgcaaggcc atcggatcgc cttgctctgc
     1561 accggaattg gcggccgcca tggcgtccag cgcctggtcc tggcgcagca ccgatggcag
     1621 cgtccgccgc gacttgggca cctgcagacg cgccgccgga tcgctggaca acaggccgcg
     1681 tcgcgtcgcc catgcggtga aggtcttcac cgccgaggtg cggcgggcca gcgtggtgcg
     1741 ggcgacacct gcggcggcct gagccgacag ccaggaccgc agcgccggca ggctcaggct
     1801 gctcagctgg gcatcgggcg tgcgctgcga aaggaagtcg aacagcgacc gcagatcgcc
     1861 caggtacgcg cggcgggtgt ggtccgagcg gccacgctcc agcgccagat ggtcgtcgaa
     1921 ctcctcgagg atcgcctcca cgcacctacc gtcgcagaga gcaggcgacc gctcaggtga
     1981 cgcgccgaag cgtttccgga gtgagatctt tgcgcgtcgg ataaccgtcg acggccatga
     2041 tcaggtcggc ttcggccaac aggcatcgca gaacgtgcac gacgccgtcc gtgccgccca
     2101 gcgcgagccc gtacgcgtac ggcctgccga tgccgaccgc ggtggcgccc agcgccagcg
     2161 ccttgacgat gtccgcgccg ttgcggatgc cggagtcgaa caggaccggc actccgttcg
     2221 ccgcttccac gacggcgggc aggcagtcga tggcggggat gccgccgttg gcctggcggc
     2281 cgccgtggtt gctgcagtag atgccgtcga cgccgccgtc gatcgcgcgt cgcgcgtcgt
     2341 cggggtggca gatgcccttc acgagcaacg gcagcttggt cagcgaccgc agccacggaa
     2401 ggtcctccca ggtgagtggg ttgccgaagg tctgaaccca gctcaggacg gcggcctgcg
     2461 ggttctcctc gggcggctgg gaaagtccgg cgcggaagac ggggtcgctg gtgtagttgc
     2521 tcaggcactt tccgcgcagt tgcgggaagt tcgaggtcga caggtcgcgg ggacgccagc
     2581 cgggcaccca ggtgtcgagt gtgacgatga tgcccttgta gccggcggcc tcggcccgct
     2641 gcacgaagct ggcggccagg tcccggttcg cgggcgtgta gagctggaaa aaccctggcg
     2701 tgtcaccgaa ttcggcggcg atgttctcca tcgggtcttc ggtcagcgtc gagatgacca
     2761 tcgggacgcc ggtgcgcgcg gcagcgcggg cggtggccac gtcgccatgg ccgttctgcg
     2821 agcagatgcc tatcacgccg atgggcgcca tgaagaccgg cgagggcagc ttcaacccga
     2881 agaggtcgac ggacaggtcg cgttccttgg cgccgacgaa cattcgcggc atgagccccc
     2941 agttatcgaa cgcggtgcgg ttgacccgtt gggtgcgctc gtcgcccgcc ccgccggtga
     3001 catacgacca caccgacggc ggcatcgcgg cttgcgcctt ggcctcccaa tcggcgaaag
     3061 ccatcggaag gttcggcacg atgccggaca ggccctgcag gtagatctcg agctggtagt
     3121 cgccgaatgc catggtctca gcgtgccagg ccgacggtca ggcgttggca ccttcctcgg
     3181 cttgagcgag ttcgcgcttc ca
//
LOCUS       DASYLX010000678         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3546010,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000678 DASYLX010000000
VERSION     DASYLX010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3546010"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /gene="asnB"
                     /locus_tag="VGP27_16035"
     CDS             complement(<1..1775)
                     /gene="asnB"
                     /locus_tag="VGP27_16035"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV7852843.1"
                     /translation="MLALLTDPSADVSAGLVEAVSGASHLMRHRGPDEPGTWHDDHIV
                     LGFNRLSIIDIAHSHQPLRWGPAESPDRYVLVFNGEIYNYLELREELASEHGATFHTD
                     GDGEAIIAAYHYWGTDALTRLRGMFAFALWDTVEGELLCARDPFGIKPLFIATGPAGT
                     AVGSEKKCLLDLAGELGIDVGIDERAVQHYTVLQYVPEPETLHRGVRRLESGSYARIR
                     PGGRPEVRRYFTPRFAAKPFTAGAEQSRYDEITAVLEDSVAKHMRADVTVGAFLSGGI
                     DSTAIAALAMRHNPRLITFTTGFEREGFSEVDVAVASAEAIGARHVTKVVSQAEFVSA
                     LPEIVWYLDEPVADPALVPLFFIAREARKHVKVVLSGEGADELFGGYTIYREPVSLKP
                     FNYLPRPLRRSMAKVSAPLPEGMRGKSLLHRGSQTLEERYYGNARSFSDAQLQAVLPG
                     FRSDWTHTDVTAPVYAESLGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLELRVP
                     FLDPEVFAVASRLPLDQKITRATTKYALRRALQPVIPSHVLNRPKLGFPVPIRHWLRA
                     GELLDWAYAMVASSGAGELVDLAAVR"
     gene            1943..3001
                     /locus_tag="VGP27_16040"
     CDS             1943..3001
                     /locus_tag="VGP27_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV7852844.1"
                     /translation="MTPRGRWVVARRAALAVVLGAAALLLSGCSWSEVLGLGWPNGIT
                     PEAHVNRELWIGSVIASLVVGVIVWGLIFWTSAFHRHKKGDTELPRQFGYNMPLELVL
                     TVVPFLIISVLFYFTVVVQEKVLHKDPNPEVVIDVTAFQWNWKFGYQKIAFRDGTFTY
                     DGADPARKAAVAGGPAGLEGPSGGEFGSGGEHGIGARKRDREYLNYDKVETLGTSNEI
                     PILVLPTGKRIEFQIASADVIHGFWVPEFLFKRDVIPDPAANNSDNKFQITEIMKTGA
                     FVGHCTEMCGTYHAMMNFEVRVVEPNDFKAYLQQRSAGKTNAEALMSINQPPTAVTTH
                     PFDTRRGEQAGATPQASK"
     gene            3009..3428
                     /locus_tag="VGP27_16045"
     CDS             3009..3428
                     /locus_tag="VGP27_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEV7852845.1"
                     /translation="MHIEARLFEFLTAFFILAAVVYAALTQLFAHGGVEWAGATALVM
                     TAGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFYSPHSWWPIIIALAASVT
                     AVGVALWLPWLIVAGVVFVLASAAGLVFEYYTGAEKH"
     gene            3595..4467
                     /locus_tag="VGP27_16050"
     CDS             3595..4467
                     /locus_tag="VGP27_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7852846.1"
                     /translation="MSGPNSPGPDSEGSESLDREPGEPTAEEDAVKDATGETEIHSRA
                     YSAPESEQFTAGPYVPADADLYEYDDYDKTELVDTGRPPRWPWVVGVVAIIAAISLVV
                     SVSLLVMGTESDNLNAAGGPTSTTSAPPVQDEITTTTPPPPPPPPSSEEPPPPPPPET
                     VTVTEPPPPAPAPPAEAPPPPAPEEPSASAAPPPTSTTRSGPRQVTYSVTGTKAPGDI
                     ITVTYIDASGRSRTQRNVYIPWSLTVTPISQSEVGSVQASSLFLVSRLNCSITTSDGT
                     VLSSNTGNSAQTSC"
     gene            4467..5162
                     /locus_tag="VGP27_16055"
     CDS             4467..5162
                     /locus_tag="VGP27_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2561 family protein"
                     /protein_id="HEV7852847.1"
                     /translation="MTYNTGAAARSRLDPTSGDAENVDRIMLAVCAAIWLAVLGSAVA
                     GTVALVDLGSGHRQSSTESGTPWLLYGVIGVSALVIVGAIPLLVRARRAALDDPRAGR
                     GGGPRPAATGRTQAVTRATDPRTQIIRSGQQRSPVAGRQRSTHPSAVGISTFGAGVPP
                     AMDRLWLRCAVATASAMGVALLAIGIATYLMAAGHDTFAWAAYVIAGLVTLAMPAIPW
                     IYLRQLHELVGTH"
     gene            complement(5193..>5631)
                     /locus_tag="VGP27_16060"
     CDS             complement(5193..>5631)
                     /locus_tag="VGP27_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEV7852848.1"
                     /translation="IGRIGMVVLPAIVYYLTYRWCIGLQRSDRAVLEHGIETGIIKRL
                     PHGAYIELHQPLGPVDDYGHPIPLEYQGAPVPKRLNKLGFGGSPGTGSFLRADPADQQ
                     EALVEAEHAGERKALTALREHQDRDGSSNGHGSSNGHGSSNGH"
BASE COUNT          948 a         1871 c         1884 g          928 t
ORIGIN      
        1 cggaccgccg cgaggtcgac cagctcgccc gcgcccgacg aggccaccat cgcgtaagcc
       61 cagtccagca gctcaccggc ccgcaaccaa tgccggatcg gcacagggaa gcccagcttg
      121 ggcctgttca gcacgtgcga cgggatcacc ggttgcaggg ctcggcgtag cgcgtacttt
      181 gtggtggccc tggtgatctt ctggtccaac ggcagccggg aggccaccgc gaacacctcg
      241 gggtcgagga acggcacccg cagttcgagc gagttggcca tcgtcatttt gtccgccttc
      301 accaggatgt cgccgcgcag ccaggtgaac aggtcgatgt gctgcatgcg cgcgaccgga
      361 tcccagccca acgactcggc atacacgggc gccgtcacat cggtatgggt ccaatcagag
      421 cggaatcccg gcagcacggc ctgcaactgc gcgtcggaga agctgcgcgc gttgccgtag
      481 taccgctctt cgagcgtctg gctgcccctg tgcagcaggc tttttccgcg catgccctcg
      541 ggcagcggcg ccgacacctt ggccatcgac cggcgcagcg gccgggggag gtagttgaaa
      601 ggcttcagcg acaccggttc ccgatagatc gtgtatccac cgaacagctc gtcggcgccc
      661 tcgcccgaca gcaccacctt gacgtgcttg cgtgcctcgc gggcgatgaa gaacagcggc
      721 accagcgcgg ggtcggcgac gggttcgtcg agataccaga cgatttcggg cagcgcggac
      781 acgaactcgg cctggctgac caccttcgtc acatgccgcg cgccgatcgc ctcggccgac
      841 gcgaccgcga cgtcgacctc cgagaaaccc tcccgttcga agccggtggt gaaggtgatc
      901 aggcgagggt tgtggcgcat cgccagcgcc gcgatggcgg tggagtcgat gccgccggac
      961 aggaatgcgc cgacggtgac atcggcgcgc atgtgcttgg ccaccgagtc ctcgagcacg
     1021 gcggtgatct cgtcatagcg gctctgctca gcacccgccg tgaacggctt ggcggcgaac
     1081 cgaggtgtga agtaccgcct cacctccggt cggccaccgg gccgaatacg ggcgtagctg
     1141 ccggactcca gccgtcgcac gccgcgatgc agcgtctcgg gttcgggcac gtactgcagc
     1201 acggtgtagt gctgcacggc gcgttcgtcg atgccgacgt cgatgccgag ttcgccggcg
     1261 aggtcgagca ggcacttttt ctcgctgccg accgccgtac ccgccggccc ggtggcgatg
     1321 aacaacggct tgatgccgaa cgggtcacgc gcacaaagca attcgccttc gactgtgtcc
     1381 cagagtgcga acgcgaacat gccgcgcagc cgggtcagcg cgtcagtgcc ccagtagtgg
     1441 taggcggcga tgatcgcctc accgtcgccg tcggtgtgaa acgtcgcgcc gtgctcgctc
     1501 gccagttcct cacgaagttc gaggtagttg tagatctcgc cgttgaagac cagcacatag
     1561 cggtccggag actcggccgg gccccatcgc agcggttgat ggctgtgcgc gatgtcgatg
     1621 atcgatagcc ggttgaagcc cagcacgatg tggtcgtcgt gccaggtgcc gggttcgtcc
     1681 ggaccgcgat gacgcatgag atgggaagcg cccgacaccg cctcgaccag accggccgag
     1741 acgtcggcgg acgggtcggt cagcagggcc agcagtccgc acaccgggcc agtatgccga
     1801 atcggtcacg cgggcgtgag cagcgatggg gagacgcacg cgaggaccgg agatagaggg
     1861 aggtgtgtcg agaccagcag ccgcgggtgg tctacgctgc gtagtattcg gcaggttcaa
     1921 ccgacttgta ggaggctgcg acgtgacacc tcgcggacgc tgggtggtgg cacggagagc
     1981 ggcattggca gtcgtccttg gtgcggctgc gctgctgctc agcgggtgta gctggtcgga
     2041 ggtgctcggc ctgggctggc cgaacggcat caccccggaa gcgcacgtga accgggagct
     2101 gtggatcggt tcggtgatcg cctccctggt cgtcggggtg atcgtatggg gcctgatctt
     2161 ctggacctcg gcgtttcacc ggcacaagaa gggcgacacc gagcttccgc gccagttcgg
     2221 ctacaacatg cccctcgagc tggtgctcac ggtggtgccg ttcctgatca tctcggtgct
     2281 gttctacttc accgtcgtgg tgcaggagaa ggtgctacac aaggatccca accctgaggt
     2341 cgtcatcgac gtgacggcct ttcagtggaa ctggaagttc ggctatcaga agatcgcgtt
     2401 cagagacggc acgttcacat acgacggcgc cgaccccgcg cgcaaggcgg ccgtcgcggg
     2461 ggggccggcg gggctggagg gcccgagcgg cggtgagttc ggctccggcg gcgagcatgg
     2521 catcggcgcc aggaaaaggg accgggagta cctcaactac gacaaggtcg agaccctggg
     2581 caccagcaac gagatcccca tactcgtgct gccgacgggc aagcgcatcg agttccagat
     2641 cgcctctgcc gacgtgatcc acggcttctg ggtcccggag tttctcttca agcgcgacgt
     2701 catccccgac ccggccgcca acaactccga caacaagttc cagatcaccg agatcatgaa
     2761 gaccggcgcc ttcgtgggcc actgcaccga gatgtgcggc acctatcacg cgatgatgaa
     2821 ctttgaggtg agggtggtcg aacccaacga cttcaaggca tacctgcagc agcgtagtgc
     2881 gggcaaaacc aatgccgaag ctctgatgtc catcaatcag ccgcccacgg cggtcaccac
     2941 tcacccgttc gacacccgcc gcggcgaaca ggcaggcgcg acgccccagg cgagcaagta
     3001 ggggatacat gcacatcgaa gccaggttgt tcgaattcct gaccgccttc ttcatccttg
     3061 ccgcggtggt ctacgcggcc ctgacgcagc tcttcgccca tggcggcgtg gagtgggcag
     3121 gcgctaccgc actggtgatg accgcaggcc tgacactcat caccggcacc ttcttcaggt
     3181 tcgtggcgcg caggctcgac accagacccg aggactatga ggacgccgag atcagcgacg
     3241 gagccggcga gctgggcttt tacagcccgc acagctggtg gccgatcatc atcgcgctgg
     3301 ccgcctccgt cacggccgtc ggcgtcgcct tgtggttgcc gtggttgatc gtcgcaggcg
     3361 tggtcttcgt gctcgcctcg gccgcaggcc ttgtcttcga gtactacacc ggcgctgaga
     3421 agcactgatc gtcggggtcc cacaccgacg gtcggcaaag gtcacatctg ggcttcagtt
     3481 gatcacctac cgcgctgcgg aattcgatcc gctcatcggc ttgggtagtg ttgccgaggc
     3541 aacgtcagcc gcttgctgtc gcgccgatga atctgtggag aaggataggc gtaaatgagc
     3601 gggccgaatt ccccaggacc ggactccgag gggtcggaga gcctggatcg ggaaccgggt
     3661 gagccgaccg ccgaggaaga cgccgtcaag gacgcgaccg gtgaaaccga gatccactcg
     3721 cgggcctatt cggcacccga gtcagagcaa ttcaccgcag gcccctatgt gcccgccgat
     3781 gccgacctct acgagtacga cgactacgac aagaccgaac tggtcgacac gggcaggccg
     3841 ccgcgctggc cctgggtggt cggcgtcgtc gcgatcatcg ccgccatctc cctggtggta
     3901 tccgtttcgc tgttggtgat gggcacggag tccgacaacc tcaacgctgc gggtggaccg
     3961 acctcgacga cgtcggcgcc cccggtgcag gacgagatca ccacgacgac tccgccaccg
     4021 ccgccgccgc caccgtcgtc tgaggaaccg ccgccgccac caccgccgga gaccgtcacg
     4081 gtgaccgagc cgccgccgcc cgctcccgca ccgcccgcag aggcgccgcc gccgcctgcg
     4141 cccgaggaac catcggcctc ggccgcgccg ccgccgacgt cgacgacgcg ctcgggtccg
     4201 cgccaggtga cctattcggt gacgggcacg aaggcgccgg gcgacatcat cacagtgacc
     4261 tacatcgacg cgtccggccg cagtcgcacg cagcgcaatg tctacatccc atggtcgctg
     4321 acggtcacgc cgatctcgca atccgaggtg ggttcggtgc aggcgtccag cctgttcctg
     4381 gtcagcaggc tcaactgctc gatcaccaca agcgacggca ccgtattgtc ctctaatacc
     4441 ggcaactccg cacagacaag ctgctgatga catacaacac tggggccgcg gcccgctcgc
     4501 ggctcgatcc gacgtccgga gacgcggaga acgtcgaccg gatcatgctc gcggtgtgcg
     4561 cggcgatctg gctcgcagta ctgggatccg cggtggcggg cacggtcgcg ctggtcgacc
     4621 tcggcagcgg acatcgacag tcctccaccg aatcgggcac gccctggttg ctgtacggcg
     4681 tgatcggcgt ttccgcactg gtcatcgtcg gcgccatacc gctgttggtg cgggcccgac
     4741 gcgcggcgct cgacgacccg cgcgcgggcc ggggtggcgg ccccagaccg gcggccacgg
     4801 gccggacaca ggccgttacg cgagcaaccg atccgcgcac ccagatcatt cggtctggcc
     4861 agcagaggtc gcccgtcgcg gggcgtcagc gcagtactca cccgtcggcc gtgggtatca
     4921 gcacattcgg cgccggggta ccccctgcga tggaccggct gtggctgcgc tgcgccgtgg
     4981 ccaccgcgtc cgccatgggt gtggcgctgc ttgccatcgg tatcgccacc tacctgatgg
     5041 cggccggcca cgacacattc gcatgggccg cttacgtcat tgcgggtttg gtgacgcttg
     5101 cgatgcccgc gattccctgg atttacctgc gtcagctgca cgaactcgtc ggcacccact
     5161 gaagggtgca cgacgcgctg cgtgcgtgtg cttcagtggc cgttcgacga gccgtgcccg
     5221 ttcgacgagc cgtggccgtt ggacgagccg tcgcggtcct gatgctcgcg cagtgcggtg
     5281 agcgccttgc gttctccggc gtgctcggcc tcgacgaggg cctcctgctg atcggcggga
     5341 tcggcgcgca ggaagctgcc ggttcccggc gagccgccga agcccagctt gttcaaccgc
     5401 ttgggcaccg gggcgccctg gtactccaac ggaatcggat gaccgtaatc gtcgactggc
     5461 ccgagcggtt ggtgcagctc gatgtaggct ccgtgcggca gccgcttgat gatgcccgtc
     5521 tcgatgccgt gctcgagcac agcgcggtcg ctgcgctgca gtccgatgca ccaccggtag
     5581 gtgaggtagt agacgatcgc gggcagcacc accatgccga tgcggccgat c
//
LOCUS       DASYLX010000679         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_364598,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000679 DASYLX010000000
VERSION     DASYLX010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364598"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VGP27_16065"
     CDS             complement(<1..1289)
                     /locus_tag="VGP27_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEV7852849.1"
                     /translation="MADAPAGLVRRVVGRIRQRDPEFDALRRALRAAAVVPLAAAVSF
                     AVAGGSQAPLFTIFGSFALMVLADFPGNTQTRAVAYAGLGLNGAVLITLGTLVSPHAW
                     LSVGLMFVLGVAVTFSGVLSETIAAGQRPTLLTFVLPACTPPGPIGERLLGWAIALAV
                     CVPAALFFLPPRHHGELRRHAAEAVAALADRLEGKATRDAVVDAMLALRENFLGADFR
                     PVGLSAGSRALVRVVEDLEWLADRVSDEAGPGMRDMQAPGVRVLRCSAAVLRHTRAAD
                     QTDARANLEAALIHLRSVARGRYRDDVESILGAPDDITASALGRQLLDRRTIAATIGA
                     TGRVIAAAAAADGRPVWARALGLRLPATTATDRLIPATTAITSTTSGFLTGRSVAVRN
                     ALRTGLGLALAVAVTHLFPVEHGFWVVLGALSVLRSSA"
     gene            complement(1282..1848)
                     /locus_tag="VGP27_16070"
     CDS             complement(1282..1848)
                     /locus_tag="VGP27_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852850.1"
                     /translation="MAGVVSREAYFETGLDVLSDLGYGGLKLAEVCNRLGVTTGSFYH
                     YFSGWPAYTRELVENWMKERTVLVIEFVKSETDPRRRIDSLIEVALQLPHGAEAAIRA
                     WSSIDAHVYDIQVQVDQQRYDFMYSSALELLANDHQAQMFARWAVYLLVGYEQATLPR
                     DTAALAWIAKQLTDALDSGRFASVPTDG"
     gene            2019..>2106
                     /locus_tag="VGP27_16075"
     CDS             2019..>2106
                     /locus_tag="VGP27_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7852851.1"
                     /translation="MPHTASRGPGRPPAAKAAETRERILRAAR"
BASE COUNT          365 a          738 c          719 g          284 t
ORIGIN      
        1 gcgctgctgc gcagcaccga cagcgcgccg agcaccaccc agaatccgtg ctcgacggga
       61 aacaggtgag tgaccgcgac ggcgagtgca agcccaaggc cggtgcgcag cgcgttgcgc
      121 acggccaccg accggccggt gagaaacccc gacgtcgtcg acgtgatcgc tgtcgtcgcc
      181 gggatcagcc ggtcggttgc ggtcgtggca ggtagccgta ggcccagcgc ccttgcccac
      241 accggccgcc cgtcggccgc ggcggcggcc gcgatcaccc tgccggtcgc tccgatcgtg
      301 gcagcgatgg tcctgcggtc cagcagctgg cgacccagtg cggacgcggt gatgtcgtcg
      361 ggcgcaccga ggatcgactc gacgtcgtcg cggtagcgtc cgcgcgccac cgaccgcaga
      421 tggatgagcg cggcttcgag gttcgcgcgc gcgtccgtct gatcggcggc gcgagtgtgc
      481 cgaagcacgg cggccgagca cctcagcacc cgcacgcccg gcgcctgcat gtcccgcatg
      541 cccgggcccg cctcatcgct aacccggtcg gcgagccatt cgaggtcctc gaccacccgc
      601 accagggcgc ggctgcccgc ggacagcccg accgggcgga agtccgcgcc gaggaagttc
      661 tcccgtaacg ccagcatcgc gtccaccacg gcgtcgcggg tggcctttcc ctcgaggcgg
      721 tcggccagcg ccgccacggc ttcggccgcg tgccgccgca gttctccgtg gtggcgcggc
      781 ggcaggaaga acaacgccgc gggcacacac acggccagcg cgatcgccca ccccagcagc
      841 cgctcgccga tcggcccggg cggtgtgcac gcgggcagca cgaacgtcag caatgtgggc
      901 cgctgaccgg ccgcgatcgt ctcgctgagc acaccagaga acgtcaccgc cacaccgaga
      961 acgaacatca ggccgaccga cagccaggcg tgcggcgaca ccagggtgcc cagcgtgatg
     1021 agaacggcgc cgttgagacc aaggcccgcg tatgccaccg cgcgggtctg ggtgttgccg
     1081 gggaagtccg ccagcaccat caacgcgaac gacccgaaga tcgtgaacaa cggcgcctgt
     1141 gaaccgccgg caacggcgaa gctgaccgcc gcggccaacg ggaccaccgc cgccgcgcgc
     1201 agcgcccgtc gcagcgcgtc gaactcggga tcgcgctgcc taatccggcc gaccacccgg
     1261 cgcaccaggc cggccggtgc gtcagccatc cgttggaacg gaggcgaatc ggccgctgtc
     1321 gagtgcgtcg gtgagctgct tggcgatcca tgccagcgcg gcggtgtccc gcgggagcgt
     1381 cgcctgctcg tacccgacca ggagatacac cgcccaccgc gcgaacatct gcgcctgatg
     1441 atcgttcgcc agcagctcca gagccgagga gtacatgaag tcgtagcgct gttggtcgac
     1501 ctgcacctgg atgtcgtaca catgggcgtc gattgagctc cacgcccgga tggcggcctc
     1561 ggcgccgtgc ggaagctgga gggcgacctc gatcaggctg tcgatccggc ggcgcggatc
     1621 ggtctcgctt ttgacgaatt cgatgacgag cacagttcgc tccttcatcc agttctcgac
     1681 gagctcgcgg gtgtaggccg gccagccgga gaaatagtga tagaaggatc cggtcgtgac
     1741 gccgaggcgg ttgcacacct cggccagttt caagccgccg tagcccaggt cggagagcac
     1801 gtccaggccg gtctcgaaat aagcctcgcg cgaaacgacc cccgccatgg gagggaacca
     1861 tagccgctgg cgcggatcac ggagcccggt ccgacatctg ctgcacggat ccgtagcaac
     1921 ggctggaaaa agggcggcat tccgcacagt ttgctgagct gtcagacaaa tcgggtgttc
     1981 tgcaaagccc cgtgatgtcg tatggcaaga tattgaccat gccgcatacg gcaagcaggg
     2041 ggccaggtcg tccaccagcc gcgaaggctg ccgaaacgcg tgagcgcatt ttgcgtgccg
     2101 cacgcg
//
LOCUS       DASYLX010000680         6737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3767536,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000680 DASYLX010000000
VERSION     DASYLX010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767536"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGP27_16080"
     CDS             complement(<1..205)
                     /locus_tag="VGP27_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7852852.1"
                     /translation="MLTRFVRNQLIIFTVAGVVGVVVMVLGYMQVPTLLGIGHLTVKL
                     ELPASGGLYRFSNVTYRGVQVGKV"
     gene            complement(205..1512)
                     /locus_tag="VGP27_16085"
     CDS             complement(205..1512)
                     /locus_tag="VGP27_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852853.1"
                     /translation="MIEKAVMRLIAVAGCVVAVATGCAFHGLNSMPLPGTVGDGSGAQ
                     IYHAEFANIGTLESNSPVMIDDVVVGSVRKMTFSDWHIDVDLSVEADVVVPANAAATI
                     GQTSLLGSLHVALDPPVGESPSGRLAPGATLPLQESASYPTTEQTLSSLSAVVNGGGL
                     GQIGDIIGNFNAALSGRQDDVRDVLTRLDKFVGVLDAQRADLITSIQALNRLAGTFAG
                     QRDVISDALLKIPPALDVLIAERPRITAALDKLRILSETSTRLVDDSQADLVTNLRNL
                     DPTIRALADVGDGLDQALATATVFPLGQNVIDRGARGDFMNLFAIIDLTIPRLKRSLL
                     LGTRWGEPGAALMPAPGEPYYLRYTYDPLMTSITGAATAPAPPGGAPQPVDGAPSLPE
                     GAPLTPGEAAPVLPQGPSPAIFAGPYGEPAAPAGAQAPPAGGG"
     gene            complement(1509..2951)
                     /locus_tag="VGP27_16090"
     CDS             complement(1509..2951)
                     /locus_tag="VGP27_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852854.1"
                     /translation="MNRRRRTAALAAAFVVLLVSGAGVLLRQTYFGPTRITAYFTSAT
                     GIYPGDEVRVLGVKAGTIAAVDPAGAQARMTIDIDHGVPIPHDAKAVIVAQSLVAARY
                     VQLTPAYVSSGPTMRDGAVIPVDRTAVPVEWDEVKAQLMRLATDLGPRTDVSGTSVAR
                     FVDSAADAMHGNGDKLRQTLAELSGVGRILADGSGNLVDIIKNLQTFVTALRDSNEQI
                     VAFQDRFATLTSVLNDDRSGLDDALTDLSAAIGDVQRFIAGSRHQTAEQVQRLASVTQ
                     NLVDHKSDLENILHVAPNAISNGYDIYNPDSGSMVGSFALNNFADPVQFVCGGIAAVA
                     DATSAETAKLCAQYLGPGLRLPNFNYPPFPINPYLTKSPSNVLYTDPAVAPGGEGPHP
                     GPTETPPAVSAYTGSADNPVPAPAEVPFPAVAPGPTAPDHLPAAPSPALYPGAPVPGP
                     PPIAAPQPESATAAPTLPQLLLPAEGSPPS"
     gene            complement(2948..4030)
                     /locus_tag="VGP27_16095"
     CDS             complement(2948..4030)
                     /locus_tag="VGP27_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852855.1"
                     /translation="MLKYRAANLIRPGIMGIILIVLVIAIGLQPERLWSWATAIRYQA
                     MFAEAGGLVLGNDVRASGMKVGSVTGVSLDAGKVLVTFSIDGKIRLGSESTAHIRTGT
                     LLGQRILAIESEGSGRLRPLDVIPISRTSSPYSLTQAVGDLTTNTAGTDTDTLNQSLD
                     TLSDTIDQIAPQLSTTFDGLTRLSRALNGRNDTLRQLLKSAGQVSGVLAERSQQLNTL
                     ILNANDLVGVLDDRREAIVGLIASTSAVARQLSGLVLDNEKELAPALDKLNSVTAMLE
                     KNRDNIAKALPGLAKYQITQGEAVANGFYYNAYEPNLSPAQFLQPFLDYAFGFRRGLN
                     AGRPPDNAGPRAELPLPYNAIPGGTR"
     gene            complement(4030..5055)
                     /locus_tag="VGP27_16100"
     CDS             complement(4030..5055)
                     /locus_tag="VGP27_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852856.1"
                     /translation="MSTHQRTGLKFGVFAVVMALLTATLFVIFGQYRTGATNGYSAVF
                     RDVSGLKVGDSVRAGGLRVGTVNELLMQPDNTVVATFDADRNVVLTTGSGAAVRYLNL
                     VGDRYLELIDGPGSTRLLQAGSQIPVDRTAPALDLDQLLGGLKPVIAGLNPQDVNALT
                     ASLLQIFQGQGGTLDSLLSSSASFSNDLADNSQVIQQLIDNLNTVLETLAKDGDKFSG
                     AIDRFEQLVSGLAADRDPIGAAIESLSTGTASIADLLAAARPPLSGTIDQLNRLAPLL
                     DQSKALVDTALQKAPNNYRKLIRLGAYGAWFNYYLCELSLRVSDLQGRTVVVPWVKQT
                     TGRCSAA"
     gene            complement(5052..6239)
                     /locus_tag="VGP27_16105"
     CDS             complement(5052..6239)
                     /locus_tag="VGP27_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7852857.1"
                     /translation="MNHRAVRPLAGVTTVLGIVTIVAIAVYLFQGGYAETVPVTVVSQ
                     RAGLVMNPDAKVKLLGAQVGRVASIEPVANGRALIHLEMDPSQLAEIPANVAVDIAST
                     TVFGAKSVNLVPPADPSPQKLKPGQVLDAGRVTVEFNTIFQQLTAVLSKIQPEKLNAT
                     LGAISSAVDGRGEKFGQALSNLDRLLASLDPSVPNLSHDVSASATALTAYADAAPDLL
                     ATADNATRISETIVDEQDNLDALLISAIGLGDIGNDVIGSNRQPLTDLLHLLVPTTEL
                     TNRYNEALTCTLSGLHRLALEPPLQEPGVVVSIGFMLGLERYRYPGNLPKVAASGGPQ
                     CLGLPVVPPNSRPPFLIADVGANPAQYGNQGILLNSDGLKQALFGPLDGPPRNTAQIG
                     QPG"
     gene            6557..>6737
                     /locus_tag="VGP27_16110"
     CDS             6557..>6737
                     /locus_tag="VGP27_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852858.1"
                     /translation="MSPTAAAGADDGSLNGRYVATSNGEWAMTNDQYRDETSVRSTWT
                     ITSSCADPTDCVGTMS"
BASE COUNT         1175 a         2090 c         2265 g         1207 t
ORIGIN      
        1 tcacctttcc gacctgcacg ccgcgatagg tgacgttgct gaaccggtag agaccaccgg
       61 aggccggcag ctcgagtttg acggtgaggt gcccgatgcc aagcagggtc ggtacctgca
      121 tatagcccag caccatcacc accacaccaa cgaccccggc gaccgtgaag atgatcagtt
      181 gattgcggac gaagcgcgtc agcatcagcc accgcccgct ggaggtgcct gcgcgccggc
      241 cggcgcagcg ggctcgccgt agggtccggc gaagatggct ggcgacgggc cttggggcag
      301 gacgggagcc gcttcgccgg gtgtcagagg agcgccctcc ggtagcgatg gcgcgccgtc
      361 gactggctgt ggcgcgccgc ccggcggagc cggggcggtc gctgcccccg tgatcgacgt
      421 catcagcggg tcgtaggtgt agcgcaggta atagggctct cccggcgcgg gcattagggc
      481 tgcgccgggc tcgccccacc gtgtacccag caacaacgag cgcttcagcc gcggaatcgt
      541 caagtcgatg atcgcgaaga gattcatgaa gtcgccgcgc gctccgcggt caatcacgtt
      601 ctggcccaac gggaacaccg tcgccgtggc cagcgcctgg tcgagcccgt ctccgacgtc
      661 ggcgagggca cgaattgtgg ggtcgaggtt gcgaagattc gtcaccagat cggcctggct
      721 gtcgtcgacc agtcgcgtgg aggtttcgct cagaatgcga agtttgtcga gcgcggcagt
      781 gatgcgcggg cgttcggcga tcagcacgtc cagtgcggga ggaatcttga gcagcgcgtc
      841 cgagatcaca tcgcgctggc cggcgaaggt accggcgagt cggttcagcg cctggatgga
      901 cgttatgagg tcggcccgtt gcgcatcgag gacgccgacg aatttgtcga ggcgcgtcag
      961 cacgtcacgg acgtcgtcct gcctgccgga cagtgcagcg ttgaagttac cgatgatgtc
     1021 gccaatctgg ccgaggcctc cgccgttgac gaccgccgac agcgacgaca acgtctgctc
     1081 ggtcgtggga tagctcgccg actcttggag tggcagcgtc gcgcccggtg caagccgccc
     1141 gctgggtgac tcccccaccg gtgggtcgag ggccacgtgc agggatccga gcagactcgt
     1201 ctgcccaatg gtggccgcgg cgttggcggg caccacgaca tcggcctcaa cggacaggtc
     1261 gacgtcgata tgccaatccg agaacgtcat ctttctgaca ctgccgacca cgacatcatc
     1321 gatcatcacc ggtgagttcg attccagcgt accgatattg gcgaactcgg cgtggtagat
     1381 ctgggcgcct gacccgtcac cgacggttcc gggcaggggc attgagttca ggccgtggaa
     1441 ggcgcatccg gtggcgaccg cgaccacgca gcctgccacc gcgatcagcc gcatcaccgc
     1501 tttctcgatc atgatggcgg cgaaccctct gcgggtaaca gcaactgcgg caacgtcggt
     1561 gccgcagttg ccgactcggg ttgcggcgcc gcgatcggtg gcggcccggg taccggcgct
     1621 ccggggtaca gggcaggcga tggagcggca ggcaaatgat cgggcgcggt cgggcccggc
     1681 gcgaccgccg ggaacggcac ctcggccgga gcggggacgg gattgtccgc cgaaccggta
     1741 tacgccgaca ccgccggcgg cgtttcggtt ggacccggat gcgggccttc accgcctggc
     1801 gccaccgccg gatcggtgta gagcacgttg ctgggcgact tcgtcaaata cgggttgatc
     1861 gggaagggag gatagttgaa gttgggcagt cgcagcccgg gcccgaggta ctgcgcacac
     1921 agcttcgccg tctcggccga ggtggcgtcg gcgacggcgg cgattccgcc gcacacgaac
     1981 tgcacgggat ccgcgaaatt gttcaacgcg aacgacccca ccatgctccc cgagtcgggg
     2041 ttgtagatgt cgtatccatt cgagatggcg ttgggcgcca cgtggaggat gttctccagg
     2101 tcgctcttgt gatcgacgag attctgggtg acgctcgcca gccgctgaac ctgttcggcg
     2161 gtttggtggc ggctgccggc gatgaatcgt tgcacgtcgc cgatcgccgc agacaggtcg
     2221 gtcaacgcat cgtcgagccc ggacctgtcg tcgttcagca cgctggtcaa tgtggcgaat
     2281 cggtcctgga aagcgacgat ctgctcattg ctgtcacgaa gggcggtgac gaaagtctgc
     2341 aggttcttga tgatgtcgac caggttgccg ctgccgtcgg ccaggatgcg cccaaccccc
     2401 gacagctctg ccagcgtttg gcgcagcttg tcgccgttgc cgtgcatcgc gtcggccgcg
     2461 ctgtcgacga accgggccac cgaggtgccg gatacgtcgg tccgcggccc caaatcggtt
     2521 gcgagccgca ttaattgggc cttcacctca tcccactcga cgggcacagc ggtcctgtcg
     2581 acgggaatca cggcaccatc gcgcatcgtg ggaccgctgg atacataagc aggggtcagc
     2641 tggacatacc gcgccgccac caggctctgg gcgacgatca ccgccttcgc gtcgtggggg
     2701 atcggtacgc cgtggtcgat gtcgatcgtc atcctggctt gggcgcccgc agggtcgacg
     2761 gcggcgatcg ttcccgcctt gaccccgagg acgcgcacct cgtctccggg atagattccg
     2821 gtggcgctgg tgaagtaagc ggtgattctg gtcgggccga agtacgtctg ccgcaaaagg
     2881 acgcctgcac cggaaacgag cagtacaaca aacgcggcag ccagcgcggc cgtacgccgt
     2941 ctccggttca ccgcgtgcct cccggaattg cgttgtaggg caacgggagt tcggcccgtg
     3001 gcccagcatt gtccggtggc cgtccggcgt tgaggccgcg ccggaacccg aacgcatagt
     3061 cgaggaacgg ctgcaggaac tgggcgggtg acaggttcgg ttcatacgcg ttgtagtaga
     3121 agccgttggc caccgcctcg ccctgcgtta tttggtactt tgccaacccg ggcagcgcct
     3181 tggcgatgtt gtcgcggttc ttctccagca ttgcggtgac cgagttgagt ttgtccagcg
     3241 cgggcgcaag ttccttttcg ttgtcgagaa ccagcccgga caactgccgc gccaccgctg
     3301 acgtgctggc gatcaagccg acaatcgcct cgcgtcggtc atcgagcacc ccgaccaagt
     3361 cgtttgcgtt gagaatgagt gtgttgagct gttggctgcg ttccgctagc acaccgctga
     3421 cctgacccgc gctcttcagt agctgccgca gggtgtcgtt gcggccgttg agcgcacgtg
     3481 acaagcgcgt cagcccgtcg aatgtcgtac tcagctgcgg cgcgatctgg tctatggtgt
     3541 ccgacaacgt atccaaggac tggttgaggg tgtcggtgtc ggtgccggcg gtgttggtgg
     3601 tcaggtcgcc gaccgcctgg gtcagcgaat acggcgacga ggtccgcgaa atcggaatga
     3661 catcgagcgg tctcaggcgc ccactgcctt ctgactcgat agccagtatg cgttggccga
     3721 gcagggtccc ggtgcggata tgcgcggtgc tctctgagcc gagccggatt ttcccgtcga
     3781 tgctgaacgt gacgaggacc ttgccggcat ccagcgaaac accggttacc gaaccgactt
     3841 tcatgccgga tgcacgtacg tcgttgccca ggacgagtcc acccgcctcg gcgaacatcg
     3901 cctggtatcg gatggccgtc gcccacgacc acagcctctc gggttgtagc ccaatggcga
     3961 tgacaagcac gatcaggatg attcccatga ttcccggccg aatgaggttg gctgcacggt
     4021 atttgagcat caggccgccg agcacctccc cgtcgtctgc ttgacccacg gaaccaccac
     4081 ggtgcggccc tgcagatcgc tgacccgcag agacagttcg cacaggtagt agttgaacca
     4141 ggcgccgtat gcgccgagcc gaatcagctt gcggtagttg ttcggagcct tctgcagcgc
     4201 ggtgtcgacg agcgccttgc tctgatcgag gaggggtgcc agccggttca gctggtcgat
     4261 cgtgcccgac aggggaggcc gcgcggcggc gaggaggtcg gcgatcgatg cggtcccggt
     4321 gctcagtgac tcgatggcgg caccgatcgg atcgcggtct gcagccagtc cgctgacaag
     4381 ctgctcgaag cggtcgatgg cgccggagaa tttgtcgccg tctttggcga gcgtttccag
     4441 caccgtgttg aggttgtcga tcagctgctg gatcacctga ctgttgtcgg ccagatcgtt
     4501 ggagaacgac gctgaactcg acagcagcga gtccagcgtc ccgccctgcc cttggaagat
     4561 ctgcaacagc gacgccgtca acgcgttgac gtcctgcgga ttgagcccgg cgatcaccgg
     4621 tttgagaccg cccaacaact gatcgaggtc caacgccggc gccgtccggt cgaccgggat
     4681 ctgagagccg gcctgcagga gtcgtgtcga tcccgggccg tcgatcaact cgaggtagcg
     4741 gtcgccgacg aggttgaggt aacgcaccgc ggctccgctg ccggtggtga gcacgacgtt
     4801 gcggtcggcg tcgaacgtcg ccaccacggt gttgtcgggt tgcatgagga gttcgttgac
     4861 cgtgcctacc cgcaggccgc ctgcccggac cgagtcaccc accttcagtc ccgagacgtc
     4921 gcggaaaacc gcggaatatc cgttcgtcgc gccggtccgg tactgcccga agataacgaa
     4981 caacgttgcg gtcagcagcg ccatcaccac tgcgaacact ccgaacttca gaccggttcg
     5041 ctgatgggtg ctcatcccgg ctgtccgatc tgggcggtgt tacgtggggg cccgtcgagg
     5101 ggtccgaaca acgcctgctt gagcccgtcg gagttgagca ggatcccctg attcccgtac
     5161 tgcgcgggat tggcaccgac gtcggcgatg aggaagggtg gacgggagtt cggcggaacc
     5221 accggcaggc ccaggcactg cgggccgccc gacgcggcca ccttcggcag gttgcccgga
     5281 taccgatatc gctccaggcc cagcatgaat ccgatcgaga ccacgacccc cggttcctgc
     5341 agtggcggtt ccaaagcaag acgatgtaaa ccggacagcg tgcacgtcag tgcctcgttg
     5401 tagcgattgg tcagctcggt ggtcggaacc aacaggtgca gtaggtcagt caacggttgg
     5461 cggttcgatc cgatgacatc gttgccgata tcgccgagtc cgatggcact gatcaggagc
     5521 gcgtcaaggt tgtcctgctc gtcgacgatg gtctcgctga tccgcgtcgc gttgtcggcc
     5581 gtcgccaaca ggtctggggc cgcgtcagcg taggcagtca gcgccgtcgc cgacgcagag
     5641 acgtcgtggc tcaagttggg cacgctcgga tcgagactgg ccagcagccg gtccaggttg
     5701 ctgagggcct gaccgaactt ctcgccgcgg ccgtccaccg ccgacgaaat tgcgccgagg
     5761 gttgcgttga gcttctcggg ctggatcttc gacaggacgg cggtcagctg ctgaaagatc
     5821 gtattgaact cgacggtgac ccgtccggcg tcgagcacct ggcctggctt cagcttctgc
     5881 ggtgagggat ccgcgggcgg caccaagttc acagacttgg cgccgaacac cgtcgtcgag
     5941 gcgatgtcga cagcgacgtt ggccggaatc tcagccagtt gagagggatc catttcgagg
     6001 tggatcaggg cccggccgtt ggctacgggc tcgatggagg cgaccctacc gacctgggca
     6061 ccaagcagtt tcaccttggc gtccgggttc atcaccaagc ccgcgcgctg cgacaccacc
     6121 gtgaccggga ccgtctccgc atagccgcct tggaataaat agacggcgat ggccacgatc
     6181 gtgacgatcc cgagaacggt tgtgaccccg gcaaggggcc ggacggcgcg gtggttcatc
     6241 gcacctcagt cgcgccgatt gagtccgtca aacgagccct cccaccggtc atgaaccttc
     6301 ggacgggtag tccgacgtcg aaccgctcct gcacggcagg ctacatcgta agggatacgt
     6361 gacgatagtt ttttggcaga tataaggtgc tactaattga cagataggcg tacaaaattg
     6421 tgtggagacc tggcgtgaaa accgcacgac gtatccaaga acgctttaac atgcggaaat
     6481 gatggttccg cctccccgag cgctcaggcg cggcgtgacg acggttggag tggccgcgac
     6541 gctagggtgc gttgccgtgt caccaaccgc tgcggccggc gccgacgatg gcagcctcaa
     6601 cggccgctat gtcgccacgt cgaacggcga gtgggcgatg accaacgacc aataccggga
     6661 cgagaccagc gtccgcagta cgtggacgat cacgtcctcg tgtgccgatc ccaccgattg
     6721 cgtcggaacg atgagca
//
LOCUS       DASYLX010000681         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1023034,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000681 DASYLX010000000
VERSION     DASYLX010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023034"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGP27_16115"
     CDS             complement(<1..291)
                     /locus_tag="VGP27_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEV7852859.1"
                     /translation="MSVTYTLLALLAFVVLTMGTAVFVAAEFSLTALERSTVDANART
                     GGRRDQIVQRAHKTLSFQLSGAQVGISVTTLITGYLAEPLIARLLEPVFAKLA"
     gene            complement(507..>1566)
                     /locus_tag="VGP27_16120"
     CDS             complement(507..>1566)
                     /locus_tag="VGP27_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HEV7852860.1"
                     /translation="FEGRPVGLVTEANCAGVDRFARVRDVAITDFVTAPVGTEPRKVF
                     DLLEHAPVDVAVMTDAAGNLAGVLTRTAAIRAGIYTPAVDARGRLRIAAAIGINGDVG
                     AKARALAEAGVDVLVVDTAHGHQVKMLDAIKAVSSLDLGLPLAAGNVVSAEGTRDLIS
                     AGASIVKVGVGPGAMCTTRMMTGVGRPQFSAVVECASAASQLGAHVWADGGVRHPRDV
                     ALALAAGASNVMIGSWFAGTYESPGDLMRDRENQPYKESYGMASKRAVAARTAGESPF
                     DRARKALFEEGISTSRMGVDPARGGVEDLLDHITSGVRSTCTYVGAHTLAELHEKVVL
                     GVQSVAGFAEGHPLPTGW"
BASE COUNT          236 a          545 c          540 g          245 t
ORIGIN      
        1 cgccagcttc gcgaagacgg gctccagcag ccgggcgatc agcggctcgg ccaggtaacc
       61 ggtgatcagc gtggtcaccg atatgcccac ctgcgcgccg gacagctgga aggacagcgt
      121 cttgtgggct cgctggacga tctgatcgcg cctaccgccg gtgcgggcgt tcgcgtcgac
      181 cgtgctgcgt tccagcgcgg tcagcgagaa ctcggccgcc acgaagaccg ccgttcccat
      241 tgtcaggacg acgaaggcca acagggccaa cagcgtgtag gtgacgctca ccgcgtcctc
      301 gtcaaatggt cgccggacca tgcgcccggc cgactagagg atggttcggc gtcgggttcg
      361 gctccggtca tgtctgttgg catcaccggg gctggcccct cgtcccggaa gctcgaggcc
      421 tcaacgggtg cctgcggcac ggattgcctt tctgtgaggg cggcgatgaa agtcctatgt
      481 tagccggtcg gaccggatcg cctcggtcac cagcccgtcg gcagtggatg gccctcggcg
      541 aacccggcca ccgactggac accgagcacg accttctcgt gtagttcggc gagcgtgtgc
      601 gccccgacgt aggtgcacgt gctgcgcacg cccgaagtga tgtggtcgag caggtcctcc
      661 acaccgccgc gggcggggtc gacacccatc cgcgacgtgg agatcccttc ctcgaacaac
      721 gccttgcggg cccggtcgaa cgggctctcg cctgcggtgc gcgcggccac cgcccgcttg
      781 gacgccatcc cgtagctctc cttgtacggc tgattctcgc ggtcgcgcat gaggtcaccg
      841 ggcgactcgt aggtgcctgc gaaccacgac ccgatcatca cgttggacgc accggccgcc
      901 agcgcgagcg cgacgtcgcg tggatgacgc acgccaccgt ccgcccacac gtgtgcaccg
      961 agctggcttg ccgccgaggc gcattcgacg accgccgaga actgtggacg gccgacgccg
     1021 gtcatcatcc tggtggtgca catggcaccg ggtccgaccc cgaccttgac gatggacgcg
     1081 cccgcgctga tcaggtcgcg ggtgccctcg gcggaaacca cgttgccggc cgccagcggt
     1141 agacccagat cgagcgagga cactgccttg atcgcgtcca gcatcttgac ctgatgcccg
     1201 tgcgcggtgt cgaccaccag cacgtcgact ccggcctcgg cgagcgcacg ggctttggcg
     1261 cccacatcgc cgttgatgcc aatcgccgcc gcgatccgca gcctgccgcg ggcgtcgacg
     1321 gcgggcgtgt agatgccggc cctgatcgcc gcggtgcggg tcagtacgcc ggccaggttg
     1381 cccgccgcgt cggtcatcac cgcgacatcg acgggggcgt gttcgagcag gtcgaagacc
     1441 ttgcgcggct cggtgccgac gggtgcggtg acgaagtcgg tgatagcgac gtcgcgcacg
     1501 cgggcgaagc ggtcgacgcc ggcgcagttg gcctcggtga ccaggccgac gggcctgccc
     1561 tcgaag
//
LOCUS       DASYLX010000682         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1573774,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000682 DASYLX010000000
VERSION     DASYLX010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573774"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1134)
                     /locus_tag="VGP27_16125"
     CDS             complement(136..1134)
                     /locus_tag="VGP27_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7852861.1"
                     /translation="MDNFQALVARKNDDRIDCAVEALAESDLPPGEVTIRVAYSSVNF
                     KDALAVTPNGGVVRDYPIVPGIDLAGEVVESQSPDFAVGDAVLAHGYEIGTGRHGGYA
                     EYARVPADWVVPLGSLSARDGAAIGTAGFTAAMSVQALIDWGIKPDDGPIVVTGATGG
                     VGSVSIDLLAAAGYEVVASTGKAEATERLKALGAADVIGRLPEDPDAKPRPLGKTRWA
                     GAVDCVGGKTLADVISSLRYGGAVAASGLTGGPGLSTTVMPFILRGVSLLGMDSVQMP
                     IGPRRDLWQKLGGSLRPQHLSDITNDVDVKDVVGVLDEIGAGRHSGRAVVKVTGGF"
     gene            complement(1164..2378)
                     /locus_tag="VGP27_16130"
     CDS             complement(1164..2378)
                     /locus_tag="VGP27_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852862.1"
                     /translation="MAINLELPRKLNAVIEMAHQGAAEMLRPISRKWDLREHEYPVEL
                     DTLATLFEGLSAANTIAFAGAEAFRDADEAKASNHNGGNMSAVLNVMEISWGDVALML
                     SVPRQGLGNAAISGVATDEQLQRLGKDVWAAMAITEPGFGSDSAAVSTTAKVDGDDYV
                     INGEKIFVTAGSRATHIVVWATLDKSLGRAAIKSFIVPREYPGVTVERLEDKLGIKAS
                     DTAVIRFDNVRIPKDNLLGSPEIQVEKGFAGVMETFDNTRPVVAAMAVGIARAALEEL
                     RKILDAAGVQISYDKPAHAQSAAAAEFLRMEADWEAGYLLTVRSAWQADNDIPNSKEA
                     SMGKAKAARVATDITLKAVEMAGTTGYSEETLLEKWARDAKILDIFEGTQQIQQLVVA
                     RRLLGLSSAELK"
     gene            complement(2416..3807)
                     /locus_tag="VGP27_16135"
     CDS             complement(2416..3807)
                     /locus_tag="VGP27_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7852863.1"
                     /translation="MTNTIDGKSARASKHNGRESAVGQHRHKRTPTDIGLALITPIVG
                     QEFLDRYNLRDPLNRGLKYGVKQVFSVAGATSRQFKKVSDSKSGPTRLKASGADYFDL
                     TPDDDQKMIVETVSEFAEEVLRPAAREADENAVYPSDLVAKATELGITAINIPEDFDG
                     IAEHRAAVTNSLVAEALAYGDMGLALPILAPAGVASALTHWGSAGQQATYLKDFAGEN
                     VPQACLAITEPRPLFDATALKTTAVRTPSGYRLDGVKSLVVAAKEAELLIVAAQLNGK
                     PALFIVESSTKGVTVKADPSMGIRAAALGQVELNNVTVPLSARLGEDAATDQDYSEAV
                     ALSRLGWASLAVGTAHAVLDHVIPYVKERQAFGEPIAHRQSVAFMCANIAIELDGLRL
                     ITWRGASRADQGLSFTREAALAKRLGTDKGMQIGLDGVQLLGGHGYTKEHPVERWYRD
                     LRAIGVAEGVVVI"
     gene            complement(3891..>4182)
                     /locus_tag="VGP27_16140"
     CDS             complement(3891..>4182)
                     /locus_tag="VGP27_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7852864.1"
                     /translation="TGWGDRRSRFLDLTDEVWRVRAYGDFWSHCLVAEGAVDIAIEPD
                     LKPWDLAPLDILVREAGGTFTNFHGEPGPHGVSAVSTNGLLHEAVLTRLNGM"
BASE COUNT          645 a         1398 c         1383 g          756 t
ORIGIN      
        1 gtccgcccga aatgacggtc ggcacaccgt cgtcgtaggg ccggtatccg gagtttccgc
       61 catcgggatc ggccgccgct gtcggcgcgg tggcgagtgc ggtggcgcat aggccgacca
      121 gaaccaaccg acgcatcaga accctccggt caccttcacc acggcccggc cgctgtgccg
      181 gcccgccccg atctcgtcga gcacgccgac cacgtccttg acgtcgacgt cgttggtgat
      241 gtccgagaga tgttgtggcc gtagcgaacc gcccagcttc tgccacaggt cgcggcgtgg
      301 cccgatcggc atctgcaccg aatccatgcc cagcagcgac actccacgca ggatgaaagg
      361 catcacggtg gtgctcaacc cggggccgcc ggtcaggccg ctggccgcca ccgcaccgcc
      421 gtagcgcaag ctgctgatca cgtcggccag cgtcttaccg ccgacgcagt cgacggcgcc
      481 cgcccatcgt gtcttgccca gcggccgcgg cttggcgtcc gggtcctcgg gcagccggcc
      541 gatgacgtcg gcggcgccaa gcgccttgag acgctcggtt gcctccgcct tgcccgtcga
      601 cgccacgact tcgtaacccg ccgcggcgag caggtcgatg ctgaccgagc cgacgccgcc
      661 ggtcgcgccc gtcacgacga tcggtccgtc gtccggcttg atgccccagt cgatcagcgc
      721 ctgcacgctc atcgcggcgg tgaacccggc cgtcccgatg gcggcgccgt cacgggcgct
      781 caacgagccg agtgggacca cccagtccgc gggcacccgt gcgtactcgg cgtagccgcc
      841 gtggcggccg gtgccgatct cgtagccgtg ggccagcact gcgtcgccga cggcgaaatc
      901 gggtgactga gactcgacca cctcgccggc aaggtcgatg cccggcacga tcggatagtc
      961 gcgcaccaca ccgccattgg gtgtcaccgc aagcgcgtcc ttgaagttga cgctggaata
     1021 ggccacccgg atcgtcacct cgccgggcgg caggtcggac tccgccaggg cctcgaccgc
     1081 gcagtcgatg cgatcgtcgt tcttgcgggc gaccagtgcc tggaaattgt ccattccccg
     1141 acactacgtc gcggcaatgg gagctacttc agttccgccg acgacaggcc aagcagcctg
     1201 cgcgccacca ccagctgctg gatctgctgc gtgccctcga agatgtcgag gatcttggcg
     1261 tcgcgtgccc acttctccag cagtgtttcc tcggaatagc ctgtggtacc agccatttcg
     1321 acggccttca gcgtgatgtc ggtggctacc cgggctgcct tggccttgcc catcgacgct
     1381 tccttcgagt tcgggatgtc gttgtctgcc tgccatgcgg accgcaccgt cagcagataa
     1441 ccggcctccc agtcggcctc catgcggagg aactccgcgg ccgcggcgct ctgggcgtgt
     1501 gcgggcttgt cgtaggagat ctgcacgcct gcggcatcga ggatcttgcg cagttcctcg
     1561 agcgcggcac gggcgatgcc gaccgccatc gcggcgacga caggccgggt gttgtcgaag
     1621 gtctccatca cgccggcgaa gcccttctcg acctggatct cggggctgcc gagcaggttg
     1681 tccttcggga tccgcacgtt gtcgaagcgg atgacggcgg tgtcggaggc cttgatgccg
     1741 agcttgtcct cgagcctctc cacggtcacg ccggggtact cgcgaggcac gatgaacgac
     1801 ttgatcgccg cgcgccccaa cgacttgtcg agcgtcgccc agaccacgat gtgggtggcg
     1861 cgcgaaccgg cggtgacgaa gatcttctcg ccgttgatca cgtaatcgtc cccgtcgacc
     1921 ttggcggtgg tcgacaccgc ggccgagtcg gagccgaacc ccggttcggt gatggccatt
     1981 gcggcccaca cgtccttgcc gagccgctgc aattgctcat cggtggccac gccgctgatg
     2041 gccgcgttgc caaggccctg acgcggcacc gacagcatca atgcgacgtc gccccaactg
     2101 atctccatca cattgaggac ggccgacatg ttgccgccgt tgtgattgct tgctttggcc
     2161 tcgtcggcgt cacggaaagc ctctgcaccg gcgaacgcga tcgtgttcgc cgccgagagg
     2221 ccttcgaaca acgtggccag ggtgtccagc tcgaccgggt attcgtgctc gcggaggtcc
     2281 cacttgcgtg agatgggccg cagcatctcc gcggcacctt gatgtgccat ctcgatcacc
     2341 gcattgagct tgcggggaag ttccaaattg attgccatga aagtgttttc cgttcctcga
     2401 ggagtgacga gtctgctaga tgacgacgac gccctcggcg acgccgatcg cgcgcaggtc
     2461 gcggtaccag cgttcgacgg gatgttcctt ggtgtagccg tggcctccga gcagctggac
     2521 gccgtccagg ccgatctgca tgcctttgtc ggtgccgagc cgcttggcga gcgcggcctc
     2581 acgtgtgaag gagaggccct gatcggcacg ggacgcgccg cgccaggtga tcagccgcag
     2641 tccgtcgagt tcgatcgcga tgttggcgca catgaacgcg accgactggc ggtgggcgat
     2701 cggctcaccg aacgcctgac gctccttgac gtagggaatc acgtggtcga gcaccgcgtg
     2761 agcggtgccg acggccagcg aggcccagcc gagtcgcgac agcgccacgg cctcggagta
     2821 gtcctggtcg gtcgcggcgt cctcgccgag acgcgcggac agcggcaccg tgacgttgtt
     2881 caactcgacc tggcccagcg cggcggcgcg gatccccatg ctcgggtcgg ccttgacggt
     2941 cacgcccttc gtcgacgact cgacgatgaa caaagcgggt ttgccgttca attgcgcggc
     3001 cacgatgagc aattcggctt ccttggcggc caccaccagg gacttgacgc cgtcgaggcg
     3061 gtagccgctc ggcgtgcgaa cggcggtggt cttcagcgca gtcgcgtcga acagcggccg
     3121 cggttcggtg atcgccaggc aggcctgcgg cacgttctcg cccgcgaagt ccttgaggta
     3181 ggtggcctgc tggccggcgc tgccccagtg agtgagcgcc gatgcgacgc cggcaggcgc
     3241 aaggatcggc agggccaagc ccatgtcacc gtaggcaagc gcctcggcga ccaacgagtt
     3301 cgtcacggcg gcacggtgtt cggcgatgcc gtcgaagtcc tcggggatgt tgatggcggt
     3361 gatgcccagc tcggtggcct tcgccacgag atcgcttgga tagacggcat tttcgtcggc
     3421 ttcgcgcgcg gcgggtcgca gcacctcttc ggcgaactcg ctgacggtct caacgatcat
     3481 cttctggtcg tcgtcgggcg tcaggtcgaa gtagtcggcg ccgctggctt tcagccgagt
     3541 cgggccgctc ttggagtcgg acaccttctt gaactggcgg ctggtggcgc ccgcgacgga
     3601 gaagacctgc ttgacgccgt acttgagtcc gcggttcagc ggatcgcgca ggttgtaacg
     3661 gtccaggaat tcttgcccga cgatcggcgt gatcaacgca aggccgatgt cggtcggggt
     3721 tcgcttgtgc ctgtgctgtc cgacagcgct ttcccgcccg ttgtgtttcg atgcgcgcgc
     3781 tgacttgccg tcgattgtgt tcgtcatttg tgtgcagcct cgctcagtag ggccagtgcc
     3841 gatcagtcta tcttactccg gagtaagtta gaagacgaat gtaacctgct tcacatcccg
     3901 ttcagcctgg tcaggaccgc ttcgtgcagc aatccgttcg tcgaaaccgc gctgacgccg
     3961 tgtgggcccg gctcgccgtg gaaattcgtg aacgtgccgc ccgcctccct cacaaggatg
     4021 tcgagaggag cgaggtccca cggcttgagg tcaggttcga tcgcgatgtc gacggcaccc
     4081 tcggccacca ggcagtggga ccagaaatcg ccgtaggcgc gtacccgcca gacctcgtcg
     4141 gtgaggtcga ggaaacgcga tcggcggtca ccccacccgg tg
//
LOCUS       DASYLX010000683         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_914693,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000683 DASYLX010000000
VERSION     DASYLX010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914693"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VGP27_16145"
     CDS             <1..513
                     /locus_tag="VGP27_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7852865.1"
                     /translation="GMSQRLGIAAALLGDPPVLLFDEPVNGLDPEGIRWVRTLMRTLA
                     GEGRTVFVSSHLLAEMSNTADRLVVIGRGRLIASTTVTEFVGRADSVRVRSPQLDTLR
                     QVLSQAGVEVDDDEAATALLVRGAASEVVGDLAARNGITLHELTSQQVSLEDAYMKLT
                     DEAVQYRAAT"
     gene            510..1229
                     /locus_tag="VGP27_16150"
     CDS             510..1229
                     /locus_tag="VGP27_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852866.1"
                     /translation="MSALAVLNAERIKLSTTRSALWSAVVVAVLSLGLAALAASTAYG
                     PGPVPPDKAAMGVAVFGVPVLMILSALTVTNEYRSAMIRTTFMAVPNRTLVLVAKAVV
                     AALFSGVYAAVMVMAAIVVAHSDPRNWRLVGAIALYAMLAAVLGVGVGALVRASAGAV
                     ALLLLWPLVAEPLLGNMPNLSSQIGPYLPFANAFTFVGVQWLYPVYAMPWGEVGSILY
                     FAAAVVVVFVAALITVNRRDA"
     gene            1264..>2756
                     /locus_tag="VGP27_16155"
     CDS             1264..>2756
                     /locus_tag="VGP27_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV7852867.1"
                     /translation="MGRKSRGVSAVLAAVVVVAAFGGCGSKPAKEADTPPSSPAASGA
                     ATEYAATLGDEVKADAMMAHLTKLQEIADAHDGNRALGSAGYDASVDYVVKTLRDKGF
                     DVQTPVFEVRLPFAEDPQLTVGGATITAKALEYTIGTPDQGVSGPLVAARVEDSPGCT
                     ASDYDGLPVAGAIVLVDRGTCQFSQKQSVAAERGAVALIVANNEDGDEMGGTLGEKTN
                     AKIPAVSVTKESGERLRGAPGESTLKLKAGVRVEKTRNVIAQTKTGSTENVVMAGAHL
                     DSVPEGPGINDNGSGVAAVLETALQLGPSPAVQNAVRFGFWGGEELGLKGSNNYVESL
                     NVDQLKDIALYLNFDMLGSPNPGYLTYDGDQSVPPNPREPNARVPEGSAGIERNFVVY
                     LKEAGETAQDTGFDGRSDYDGFTLAGVPAGGLFSGAEEKMTREQAGLWGGKADEPFDP
                     NYHKKTDTLDHVDRKALQINGSGVGYVVGLYAQDQRGRNGVPVRDDRT"
BASE COUNT          429 a          885 c         1012 g          430 t
ORIGIN      
        1 ggcatgagcc agcggctcgg catcgcggcc gccctgctgg gtgatccgcc ggtgctgctg
       61 ttcgacgagc cggtcaacgg gctggacccc gagggcatcc ggtgggtgcg cacgctgatg
      121 cgcacgctcg ccggcgaggg ccgcaccgtt ttcgtgtcga gccatctgct cgccgagatg
      181 tccaacaccg ctgaccggtt ggtggtgatc gggcgcggca ggctgatcgc gtcgacgacg
      241 gtgaccgagt tcgtcggccg cgccgacagc gtccgggtgc gcagcccgca gctggacacg
      301 ctgcggcagg tgctgtcgca ggcgggtgtc gaggtcgacg acgacgaggc ggccacggcg
      361 ctgctggtgc gcggcgcggc cagcgaggtg gtcggcgacc tggccgcgcg caacggaata
      421 accttgcacg agttgacctc acagcaggtg tcgctggagg acgcatacat gaagctcacc
      481 gacgaagcgg tgcagtaccg ggcggcgaca tgagtgcttt agcggtgctg aacgcggaac
      541 gcatcaagct gtcgaccacc cgatcggcgc tgtggtcggc ggtcgtggtg gcggtgctga
      601 gcctcgggct ggccgcgctt gcggcgtcga ccgcctatgg tcccggcccg gtgccgccgg
      661 acaaggcggc aatgggtgtc gccgtcttcg gggtgccggt gctgatgatc ctgtcggcgc
      721 tgacggtcac caacgagtac cgcagcgcaa tgatccgcac cacgttcatg gccgtgccga
      781 accgaacgct ggttctggtg gcgaaagcgg ttgtcgcggc gctgttctcg ggtgtctacg
      841 ccgcggtgat ggtgatggcc gccattgtgg tggcgcactc ggatccccga aactggcgtc
      901 tggtcggtgc gatcgcgctg tacgcgatgc tcgcggcggt gctcggcgtc ggcgtcggcg
      961 cactggtgcg ggcctccgcg ggcgcggtgg ctctcctgct gctgtggccg ctggtcgccg
     1021 agccgctgct gggcaacatg cccaacctga gttcgcagat cggcccctac ctgccgttcg
     1081 cgaatgcgtt cacgttcgtc ggcgtgcagt ggctctatcc ggtctacgcc atgccgtggg
     1141 gcgaagtcgg gtcgatcctg tacttcgcgg ccgcggtggt ggtcgtgttc gtcgcggcgc
     1201 tcatcactgt gaaccgacgt gacgcgtgag ccgaggcgga aagaaccgct aatgttggga
     1261 gcaatgggtc gcaaatcgcg cggggtgtcc gctgtgctcg ccgcggttgt cgtggtggct
     1321 gcgttcggcg gctgcggaag caagcccgcg aaggaggccg acactccgcc gtcctcgccc
     1381 gcggcgtccg gcgcggcgac cgaatacgcg gcgacgctgg gcgacgaggt caaagccgac
     1441 gcgatgatgg cgcacctgac gaagctgcag gagatcgccg acgcccacga cggaaaccgc
     1501 gcgctgggca gcgccggcta cgacgccagc gtcgactatg tcgtcaagac gctgcgcgac
     1561 aagggtttcg acgtgcagac gcccgtgttc gaggtgcgcc tgccctttgc cgaggatcca
     1621 cagctcaccg tcggcggcgc gacgatcacg gccaaggcgc tcgagtacac catcggcacg
     1681 cccgaccaag gcgtctcggg gccgctggtg gccgcccgcg tcgaggattc gccgggctgc
     1741 accgcatcgg actacgacgg gctgccggtt gcgggcgcga tcgtgctggt ggaccgcggc
     1801 acctgccagt tctcgcagaa gcagtccgtg gcggccgagc gcggagcggt cgcactgatc
     1861 gtcgccaaca acgaggacgg cgacgagatg ggcggcaccc tgggtgagaa gaccaacgcg
     1921 aaaatcccgg ccgtcagcgt caccaaggag tcgggggagc ggctgcgcgg cgcaccgggt
     1981 gagtcgacgc tgaagctcaa ggccggcgtc cgcgtcgaga agacccgcaa cgtgatcgcc
     2041 cagaccaaga ccggctcgac cgagaatgtg gtgatggccg gcgcccacct ggacagcgtg
     2101 ccggagggtc ccggcatcaa cgacaacggt tccggggtgg ccgccgtcct ggagaccgct
     2161 ctgcagctgg gtccgtcgcc tgcggtgcag aacgcggtgc ggttcggctt ctggggcggc
     2221 gaggagctcg gcctgaaggg ttcgaacaac tacgtcgagt cgctgaatgt cgaccagctc
     2281 aaggacatcg cgctgtacct gaacttcgac atgctgggct cgcccaaccc cggctacctc
     2341 acctacgacg gtgatcagtc ggtgccgccg aaccccaggg agcccaacgc gcgggtgccc
     2401 gaggggtcgg cgggcatcga gcgcaacttc gtcgtctacc tcaaggaggc gggcgagacg
     2461 gcgcaggaca ccgggttcga cggccgctcc gactacgacg ggttcactct ggcgggcgtt
     2521 cccgcgggcg gcctgttctc cggcgccgag gagaagatga cgcgcgaaca ggccggattg
     2581 tggggcggca aggccgacga gccgttcgac ccgaactatc acaagaagac cgacaccctc
     2641 gaccacgtcg atcgtaaggc gttgcagatc aatggttccg gcgtcggcta cgtcgtcggt
     2701 ctctacgcgc aggatcagcg cggccgaaac ggggtgccgg ttcgcgacga ccgcac
//
LOCUS       DASYLX010000684         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2668902,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000684 DASYLX010000000
VERSION     DASYLX010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668902"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..256
                     /locus_tag="VGP27_16160"
     CDS             2..256
                     /locus_tag="VGP27_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV7852868.1"
                     /translation="MLFELVLIARMVVDWIGVLSPVSGSSGLYQARRITHGITEPVIG
                     PVRRVLKPARLGSVSIDLAFTAVFVAVIVLRTVVVPLIPF"
     gene            complement(320..508)
                     /locus_tag="VGP27_16165"
     CDS             complement(320..508)
                     /locus_tag="VGP27_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852869.1"
                     /translation="MDRDFYRGVSAEAALITRFVERSARSNAALEHAARTSNVRMIRV
                     SGDYPPDTVCTRVLNAIT"
     gene            851..1702
                     /locus_tag="VGP27_16170"
     CDS             851..1702
                     /locus_tag="VGP27_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEV7852870.1"
                     /translation="MHNDNITPLIGIFDMYSASLAAEHYGGMFVSGFGFAASYYGLPD
                     IGFIAWPDMVSFVQRLRLAFPQQHLLVDIDDGYVDPEVACHVVEHLERIGASGVILED
                     QKRPRRCGHVTGKQILPIEEYLEKLNLVLETRTELVVVARTDATEESEILRRAEMLAA
                     TDADVLLVDGVRSAEWIRKVRGVVGNKPLLFNQIAGGLSPRLSLTELEELGVNVAIYS
                     TPCLFAAHTAIANALVELRANDGRLAEVKSDDVGVATSIGLLERNLSRHHPRRRLDSP
                     QQLRAAV"
     gene            1702..2754
                     /locus_tag="VGP27_16175"
     CDS             1702..2754
                     /locus_tag="VGP27_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEV7852871.1"
                     /translation="MFRLRVDPYILLLLTTVGVASLLPARGIVAVECRHATAIAIGLL
                     FFLYGARLSTRAALEGLRHWRLHGVVLLFTFVVFPVLGLICRVLVPDVLTPELYAGVM
                     FLCCLPSTVQSSIAFTSIAKGNVAAAICSASFSNLIGIVLTPVLVTVFLVTQTGGGVS
                     LTAVRDIVLQLLVPFLAGQVLQRWIGGFVSRHKKVLGYVDRGSILLVVYAAFSEGVVA
                     GIWGRLTIPSLVLLFLTNAAILGTVIGSTMWTARRLGFSTEDQIAIVFCGSKKSLASG
                     LPLASLLLPAATAGLMVVPLMMFHQMQLMVCAWLARRYGARRDPSTSQQISPANSRAR
                     TALAISAPGPSVDAGT"
BASE COUNT          520 a          931 c         1029 g          697 t
ORIGIN      
        1 cttgctgttc gaactcgtgc taattgcccg gatggtggtc gactggatcg gcgtgttgtc
       61 cccggtgagc ggcagcagcg ggctctacca ggctcgccgg atcacccatg gcatcaccga
      121 gcccgtgatc ggcccggtcc ggcgggtgct gaagccggcg cggctcgggt ccgtatccat
      181 tgatctggcg tttaccgcag tgtttgtcgc tgtgatcgtc ctacgaaccg tcgtggtgcc
      241 gttgattccg ttctgagcgc gcggcctcag gggctgaacg tcgcatcgtc cgccccgtgg
      301 aggagactgt cgatctgtgc tacgtgatcg cgttgagtac tcgcgtgcac accgtgtctg
      361 gtggatagtc tccgctgacc cgaatcatgc ggacgttgct tgtccttgcg gcgtgctcca
      421 gcgccgcgtt ggagcgtgcc gaacgctcga cgaacctcgt gatcagtgcc gcttcggcgg
      481 acacgccgcg gtagaagtct cgatccatac gtagccgccg cgtcagggtt cacggcgcgc
      541 acggcggcga caagatcgtc ggtgcagacg gtgtcatatc cgagtcctac gccaatcttc
      601 ctgcggccga actcttaccg gagcgcagcg ctccaccgag cagtaggtcg ccacgaagcc
      661 cagccgaacc atcggtgccg gtgccaggaa atgtgctgag gctgtctcag cgcactttca
      721 gcgcaactca cgcacagttt ctgtactggg ctgaaaccag ttcaaagcag gctgaggcgt
      781 ggatatttgg agacttcgcc tcgatgacta ggagtacaac atgggatatg gagatgcgct
      841 tcgcgacgag gtgcacaacg acaacatcac tccgctgata gggatattcg acatgtattc
      901 cgcttcattg gcggcggaac actatggcgg gatgttcgtg tcgggcttcg gtttcgcagc
      961 ttcctattac gggctgcccg acattgggtt catcgcctgg ccggacatgg tcagcttcgt
     1021 gcaacggttg cgtctcgcct tcccgcaaca gcacctattg gtggatatcg acgacggcta
     1081 tgtcgacccc gaggtggcct gccatgtggt ggagcatctg gaacgcatcg gtgcatccgg
     1141 tgtgattctg gaggaccaga agcggccacg ccgctgcggc catgtcaccg gcaagcagat
     1201 cctgcctatt gaggagtact tggagaagct gaacctggtg ctggaaacca ggaccgaact
     1261 tgtagtggtg gcccgcactg acgcgacgga ggagtccgaa attctgcgtc gggctgaaat
     1321 gctggcagcc accgatgccg acgtcttgct ggtcgatggc gtgcgaagcg cagagtggat
     1381 acgcaaggtg cgcggcgtgg tgggcaataa gccactgctg ttcaaccaga tcgcgggcgg
     1441 cttgtcgccc cgactctcgc tgaccgagct cgaggagctt ggcgtgaacg tggcgatcta
     1501 ttcgacaccg tgtttgttcg cggcgcacac cgcgattgcc aatgcattgg tcgagctgcg
     1561 cgccaacgat ggccgtcttg ccgaggtcaa gtccgatgac gtgggtgtgg cgacctcgat
     1621 agggttgttg gaacgtaatc tttcccggca tcacccacgc cgtcggctcg actcgcccca
     1681 gcagttgcgc gctgcggtct gatgtttcgg ctccgggtcg atccctacat tctgctgctg
     1741 ctcaccaccg tcggagtggc atcgctgttg cccgcacgag gcatcgtcgc tgtggaatgt
     1801 aggcacgcca cggccatcgc gatcgggcta ttgttcttcc tgtacggcgc tcggctgtca
     1861 acgcgtgctg cgctggaagg attgcggcac tggcggttgc atggcgtggt gttgttgttc
     1921 accttcgtgg ttttcccggt gctggggctg atctgtcgtg tattggtgcc ggatgtattg
     1981 acgcccgagt tgtacgcggg ggtgatgttc ttgtgctgcc tgccgtcgac ggtgcaatcg
     2041 tcgattgctt tcacctcgat tgcaaaaggc aatgtcgcgg cggcgatatg cagcgcgtcg
     2101 ttctccaacc tgatcggcat cgtgttgacg ccggtattgg tcacggtgtt cctcgtcacc
     2161 cagaccggag gcggggtgtc gctcacggcg gtccgcgaca tagtgctgca gttgttggtg
     2221 ccgttcctcg cgggacaggt gctacagagg tggatcggcg gattcgtttc ccggcacaag
     2281 aaggtgcttg gttacgtcga ccgcggatcg attctgctgg tggtgtacgc ggctttcagc
     2341 gaaggtgtgg tagccgggat ttggggccgc ctcaccattc cttcgctggt gttgttgttc
     2401 cttaccaacg ccgcaatcct gggaaccgtg atcggatcga ccatgtggac ggcacgccgc
     2461 cttggcttct cgaccgagga tcagatcgcg atcgtgttct gcggctcgaa gaaatcactg
     2521 gccagcggat tgcccttggc cagcctgttg ctgcccgcgg ccacggcagg gttgatggtc
     2581 gtgccgttga tgatgttcca ccaaatgcag ttgatggtgt gcgcgtggct ggcccgacgg
     2641 tacggcgcgc gccgcgaccc atccacgtcg caacagattt ccccggcgaa cagccgtgcg
     2701 cgaaccgccc tcgcgatcag tgcccctggt ccttctgtgg atgctgggac ctagccacgt
     2761 ccggggcatg tgccgacgac gggtgatagc tggacgccga cgtggagacg ccgactgcgg
     2821 atacagttca gccggaatca cgttgacgtc ggcggccgtg tagaggacga ggaaccaagg
     2881 agcacgacgc tttccgatgc tcctctggca gtcctccagc gcccccgcga gccggaattc
     2941 tcgcctgcct gcccgcgtac aaggcacggt aattggggac cggtccgggt gcttgcccct
     3001 aacggcgaat caccatttgc tccgaatctg tgcgggcctg catctctcat gtcggcggct
     3061 tgcagaagta tgaaccggtc tgcgctcgag atcagccttc cgattggtag cgggagcgcg
     3121 agcctgaact cagacatctc cattcggttc tcccatcgag gggttgcacg cgtttcg
//
LOCUS       DASYLX010000685         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2450308,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000685 DASYLX010000000
VERSION     DASYLX010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450308"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGP27_16180"
     CDS             complement(<1..485)
                     /locus_tag="VGP27_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218091.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7852872.1"
                     /translation="MSVLAESELGSEAQRERRKRILDATLAIASKGGYEAVQMRAVAE
                     RADVAVGTLYRYFPSKVHLLVSALGREFERIDAKTDRAALAGGTPYQRLNLMVGKLNR
                     AMQRNPLLTEAMTRAFVFADASAAGEVDHVGKLMDSMFARAMSEGEPTEDQYHIARVI
                     S"
     gene            760..>1953
                     /locus_tag="VGP27_16185"
     CDS             760..>1953
                     /locus_tag="VGP27_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7852873.1"
                     /translation="MSQASATATDEQFAARELVRSWAAGSTAVKAAREVEQGDAESWR
                     APYASLAQLGIFGVALPEEFGGAGGTVDDLCAMVEEAAAAMVPGPITGTAVATIVVPS
                     SRADLLEALASGRRTAGLTLSADVTFDDGRASGTAEYVSGATADGLLLLPAGEKFILV
                     DAAADGVAVEPLTATDFSRPLARVVLADAPAEALPVSQRRVEDLAATVQAAEAAGLAR
                     WTLHTATEYAKVREQFGKPIGSFQAIKHMCAEMLLRSEQAAVAAGDAARAANGSDESQ
                     LSIAAAVAAAAGIEFAKANAKDCIQVLGGIGITWEHDAHLYLRRAYGISQFLGGSSRW
                     LRRVAALTQQGVRRELHIDLASVEHLQPEIAAAVADVASQPAEKRQIALAEAGLQAPH
                     WPAPYG"
BASE COUNT          301 a          668 c          676 g          308 t
ORIGIN      
        1 tccgagatca ccctggcgat gtggtactgg tcctcggtcg gctcaccctc gctcatggcg
       61 cgcgcgaaca tcgagtccat cagcttgccg acgtgatcga cctcgccggc agccgatgcg
      121 tcggcgaaca cgaaggcccg ggtcatcgcc tcggtcagca gcgggttgcg ctgcatcgcc
      181 cggttcagct tgccgaccat gaggttgagc cgctgatacg gcgtgccgcc cgcgagcgca
      241 gcgcggtcgg tcttggcgtc gatgcgctcg aactcgcggc cgagggcgga caccagcagg
      301 tgcaccttgg acgggaagta gcggtacagg gtgccgacgg cgacgtcggc gcgctctgcc
      361 accgcacgca tctgcacggc ttcataaccg cccttggagg cgatggcgag ggtggcgtcg
      421 aggattcgct tgcggcgctc gcgctgggct tccgacccga gttcggactc ggcgagcaca
      481 gacaccggca tcacctgggg tggccgggac tctgaacccg aagacgggtt gggttgtgct
      541 ggctgtggca tcccggacat ccggcggaca gctccttcat catgcggtgt cgtaggaaac
      601 gatacgcatg ccgatccatg ttttctgact tcagtgccta ttgcccaccg atgtgtcctg
      661 ctgtaacggc gttcgacttg acgctcgatg gctggcacta ttagaacacg ttctagtgag
      721 aagtacgccc ttcccacgcg aaggctgagg agactccaaa tgtcgcaggc gtcagcgacc
      781 gccaccgacg agcagtttgc cgcgcgtgag ctggtccgca gctgggccgc ggggtccacc
      841 gccgtcaagg ccgcccgcga ggtcgagcag ggcgatgcgg aatcgtggcg tgcgccatac
      901 gcgagcctgg cccaactagg gatcttcggt gtggccctgc ccgaggaatt cggcggggcc
      961 ggtggcacgg tcgacgacct gtgcgcgatg gtcgaggagg ccgccgccgc gatggtgccc
     1021 ggcccgatca ccggcaccgc cgttgccacc atcgtcgtgc cgagttcgcg ggccgacctg
     1081 ttggaggcgt tggcctccgg ccggcgcacc gcaggactca cgctgagcgc cgacgtgaca
     1141 ttcgacgacg gccgggcctc cggcaccgcc gaatacgtgt ccggtgccac cgccgacggg
     1201 ctgctgctgc taccggccgg cgagaagttc atcctcgtcg atgccgccgc cgacggcgtg
     1261 gccgtcgaac cgctgaccgc gaccgacttc tcccgcccac tggcccgggt cgtcctcgcc
     1321 gacgcacccg ccgaggcgct gccggtttca cagcgacggg tcgaggacct cgctgcgacg
     1381 gtgcaggccg ccgaggcggc cgggttggca aggtggacgc tgcacaccgc caccgaatac
     1441 gcgaaggttc gcgaacagtt cggtaagccg atcggcagct tccaggcgat caagcacatg
     1501 tgcgccgaga tgctgctgcg ttccgagcag gccgcggtgg ccgcgggcga tgcggcgcgc
     1561 gcggccaacg gctccgacga atcacagctg tcgatcgccg ccgccgtcgc ggccgccgcg
     1621 ggcatcgagt tcgccaaggc caacgccaaa gactgcatcc aagtgctcgg cggtatcggc
     1681 atcacctggg agcacgacgc acacctgtat ctgcgccggg cgtacggcat ttcgcagttt
     1741 ctcggcggct cgtcgcgctg gttgcggcgg gtcgcggcgc tgacacagca aggcgtgcgc
     1801 cgcgaactgc acatcgactt ggcatccgtc gagcacctgc aacccgagat cgccgccgcg
     1861 gtggccgatg tcgcgtccca gcccgccgag aagcggcaga tcgcgctcgc cgaggccggg
     1921 ctgcaggcac cgcactggcc ggcgccgtac ggc
//
LOCUS       DASYLX010000686         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3109614,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000686 DASYLX010000000
VERSION     DASYLX010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109614"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2626)
                     /locus_tag="VGP27_16190"
     CDS             complement(<1..>2626)
                     /locus_tag="VGP27_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEV7852874.1"
                     /translation="GVFGLEDGALLMAERGRLFGSLPAGGRMVAVFAAAERVESLTDE
                     FPSLSVAAYNGANTALSGPADDLHRAVAGLTSDGVRCDWLDTSHAFHSALLEPILDEF
                     ESYAERLNYKTPQRILIDNRTGTALGRSVKLDGSYWRRHARQPVEFAKSVRTLAELNC
                     KVLLEIGPQPVLTAAALRAWPDPATAPRAIASLRRKTSDQRQITEAVADAYVLGHLPD
                     FGAFRQAHARKLDLPTYPFERRQYWYSDNRERATRQQHLDGPRTEAVRLLEDGRIEEL
                     AALLDGASGDLQTLDVLTKLAAQHNQQRTNQSLADDRYEFRWQKSTVPTSSADAGEDC
                     AWILVGDGPDAVQPLVDVLTARGHRYRILGLPVSDADEEELADALRTAAADDPALRIV
                     HVAALDSETAPSMRSLLQMQHRILGGTRRLFRAAAAAELRIPIWVVTRGAQRATDADT
                     VSPDQSCLWGFGRAAALELPQVWGGLADLSGGTADEWASFINRITPPRGSAIREDQLA
                     LRGQAVYVPRLVRRVGQPNTKPVELRDDATYLVTGGLGSIGLEIAGYLAARGANHLVL
                     TSRHAPSDAVQQRIDALGEQHGCEFRVVTADVADAHDVARLLATMRTELPPLAGIVHA
                     AGEIGTTPLSNLDDAEVDRVFAGKVWGAWHLSEAAADLQLDFFISTSSIASVWGGFGQ
                     TAYGAANAFLDGMAWRLRQQGICGISVNFGPWSAGMADEESRARLDQRGVRTLSPADA
                     LAGLADLMTGSAAQGVVARIDWARFLPLYQQAGRRAFLAELEREVPAQLAGVAPPATL
                     SGKTAFVERLTDAPVQQRRKLMTDYLRDAVAEVTRVDPAEIREDAGFFDLGMDSLMAV
                     ELRRRIEQGIGKEIPVTLV"
BASE COUNT          426 a          874 c          879 g          447 t
ORIGIN      
        1 caccagagtg accggtatct ccttgccgat tccctgttcg atgcggcgcc gtaactcgac
       61 ggccatcagc gaatccatgc cgaggtcgaa aaaccccgcg tcctcgcgga tctccgcggg
      121 atccacgcgc gtcacctctg ccaccgcgtc gcgcaggtag tcggtcatga gctttctgcg
      181 ctgctgcacc ggagcgtcgg taagccgctc gacgaacgcg gttttcccag acagagttgc
      241 cggcggcgcc actccggcca gctgggcggg gacctcgcgc tccaactccg ccaggaatgc
      301 gcgcctgccc gcctgctgat acagcggaag gaaacgggcc cagtcgatcc gggccacgac
      361 accctgtgct gccgagcccg tcatgaggtc ggccagaccc gccaatgcat cggcgggtga
      421 cagtgtccgg accccgcgct gatcgaggcg cgcacgggac tcctcgtcgg ccatgcctgc
      481 cgaccagggg ccgaagttga cgctgatccc gcagatgcct tgctgccgca gccgccaggc
      541 catgccgtcg aggaaggcat tggctgcgcc gtaggcggtc tgaccgaatc caccccatac
      601 cgaggcgatc gacgaggtgc tgatgaagaa gtcgagttgc aggtcagctg ccgcttcgct
      661 caaatgccat gcaccccaga cctttcccgc gaagacgcga tccacttcgg cgtcgtccag
      721 gttgctcagt ggggtggtac cgatctcgcc cgccgcatgg acgatgccgg ccaacggcgg
      781 tagctcggtc cgcatggtgg ccagtaggcg tgcgacgtcg tgcgcgtcgg cgacatcggc
      841 ggtgacgacg cggaattcac agccgtgttg ttcgccgagc gcatcgatgc gctgttgcac
      901 ggcatcgctg ggcgcgtgtc ggctggtcag cacgaggtgg ttggcgccgc gggcggccag
      961 gtagccggcg atctccagtc cgatcgagcc caggccgccg gtcaccaaat acgttgcgtc
     1021 atcgcgcagt tccaccggtt tcgtattcgg ctgcccaacc cgtcgaacca accgcggaac
     1081 atagaccgct tgaccgcgaa gcgcgagctg gtcttcccgg atggccgaac cgcgcggcgg
     1141 cgtgatccgg ttgatgaacg aggcccattc gtcggcggtg ccgccggaca gatccgccag
     1201 tccgccccac acctgcggaa gctccagtgc tgcggcccga ccgaatcccc ataggcaact
     1261 ctgatccggc gacacggtgt ctgcgtcggt ggctcgttga gcgcccctgg tcaccaccca
     1321 gatgggtatg cgcagttcgg cggccgcggc ggcacggaat agccgccgag ttccgcccag
     1381 gatccggtgt tgcatctgca acagagaccg catcgatggt gcggtctcgg agtcgagggc
     1441 cgcgacatgc acgatgcgta gcgccggatc gtctgctgcc gctgtgcgca gcgcgtctgc
     1501 gagttcttcc tcgtcggcgt cggacaccgg caacccgagg atccggtacc ggtggcctcg
     1561 tgcagtcagc acgtcgacca acggctggac cgcatcgggc ccgtcgccga caaggatcca
     1621 cgcgcaatcc tcgccggcgt ccgcgctgga ggttggaaca gtggatttct gccagcggaa
     1681 ctcgtagcgg tcgtcggcca gggactggtt tgtccgttgt tggttatgtt gtgccgcaag
     1741 cttggtcagc acgtccaagg tctgaaggtc gccgctggca ccgtcgagca aggccgcgag
     1801 ttcttcgatc cggccgtcct cgagcaggcg gacggcttcg gtacgcggtc cgtcgaggtg
     1861 ctgttgccgg gtggcccgct cccgattgtc gctgtaccag tactggcgac gctcgaacgg
     1921 ataggtcggt aggtcgagct ttcgcgcgtg cgcctgccgg aacgcaccga agtccggcag
     1981 atggcccaag acatatgcgt cggcgacggc ttcggtgatc tgtcgctggt cggaggtttt
     2041 ccggcgcagg gacgcgatcg cccgcggagc ggtggccggg tcgggccatg cccgcagagc
     2101 tgcggcggtg agcaccggtt gtgggccgat ctccagcaac actttgcaat tcagttcggc
     2161 aagggtgcgc acgctcttgg cgaactccac cggttggcgc gcgtgacggc gccaatagga
     2221 gccatcgagt ttaacgctcc taccgagcgc ggtcccagtg cggttgtcaa tgagaatccg
     2281 ttgtggcgtc ttgtaattca agcgctccgc atacgactcg aactcgtcga ggatcggttc
     2341 cagcagcgcc gagtggaacg cgtggctggt gtccagccag tcacaccgga cgccgtcgga
     2401 cgtcaacccg gccaccgccc ggtgcaaatc gtcagcgggt cccgacaggg cggtgttggc
     2461 gccgttgtaa gcggcgaccg acagactcgg gaactcgtcg gtgaggctct cgacgcgctc
     2521 ggcggcggcg aacaccgcga ccatccggcc gcccgcaggc aaactgccga agaggcggcc
     2581 gcgctcggcc atcagcagcg cgccgtcctc gagaccgaat acaccc
//
LOCUS       DASYLX010000687         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1023774,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000687 DASYLX010000000
VERSION     DASYLX010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023774"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..556
                     /locus_tag="VGP27_16195"
     CDS             167..556
                     /locus_tag="VGP27_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852875.1"
                     /translation="MRPGQARSRSDLLALQAVLRAHHPAARCPEAHGTREVKLRCDRC
                     WDMQIGQRARDYDLTTLRVHVDHRDASHPCARCGHHETELHHFSSAALFGWEESQRWP
                     TAWLCPRCHRRWHRVMASGASMSAAEA"
     gene            589..1014
                     /locus_tag="VGP27_16200"
     CDS             589..1014
                     /locus_tag="VGP27_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852876.1"
                     /translation="MISIRKNRDRLVGEPGPIAVELYAAGTPPMRGVAAKRPIDPRVS
                     TVRIGDELRVERRGDEWAALGDQPHRPFPQLRRVLPVRPGVTPSSSKTGVSGLAGAVH
                     SRLVGLRGTAPWDNANMLEFRPACENCNRPLAPDAMICS"
     gene            1481..>1851
                     /locus_tag="VGP27_16205"
     CDS             1481..>1851
                     /locus_tag="VGP27_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852877.1"
                     /translation="MARRGQRQTGTRRTALRNQRRVNDRDDVISVLARAVREVEAAAQ
                     RGSVTPAVRTRFQVVALLLREEHARLRADATSSDTHRAEQLKRLDGIATILARTAVRD
                     PALLALLADDAVVSDAARSLKR"
BASE COUNT          276 a          654 c          635 g          286 t
ORIGIN      
        1 gccatcgtcg cccgcgaccc tgccgtggtg gaacggatcg acgagtgggg gtggcaggag
       61 ggcctgaagc cgggtccgca ggcccccgtc tggcggatgg acgccttccg tgaccggttc
      121 ctgaccacct tcgagcacta gccgtgacca tcccgctctg cgggcagtgc ggccgggaca
      181 agctcggtcc cggtccgatc tgctggcact gcaagcagtc ctgcgagcac atcacccggc
      241 agcccgttgt ccagaagcgc acggcacgcg cgaggtgaag ctgcgctgcg accgctgctg
      301 ggacatgcag atcgggcagc gggcgcggga ctacgacctg acgaccctgc gggtgcacgt
      361 cgaccaccgg gacgctagcc acccatgcgc gcgctgcggt caccacgaga cggagctgca
      421 ccacttctcg tccgccgccc tgttcggctg ggaggaatcg cagcgctggc cgacggcgtg
      481 gctgtgccct cgctgtcacc ggcgttggca tcgggtcatg gcctcgggcg cttcgatgtc
      541 ggcggccgaa gcttgaccgg cgttcggcct gatccgaaca cgtaccccgt gatctcgatc
      601 aggaagaaca gggatcgatt ggtcggcgag cccggcccga tcgcggtgga gctgtatgcc
      661 gccgggacgc ccccgatgcg cggcgtcgcg gcgaagcgac ccattgatcc gcgcgtgtcc
      721 acagttcgca tcggtgacga actgcgcgtc gagaggcgcg gcgacgaatg ggcggcactc
      781 ggcgaccagc cgcaccgccc gttcccgcaa ctccggcggg tactgcccgt gcgtcctggc
      841 gtgactccat cctcctcaaa aactggagtc tccggactcg ccggggcggt tcactcccgc
      901 ctggtcggtc tgcgcgggac cgctccctgg gataatgcga acatgctaga gttccgaccc
      961 gcgtgcgaga actgcaaccg cccgctcgcg cccgacgcga tgatctgctc gtaggagtgc
     1021 acgttctgcc gggactgcgt cgacacggtc ctgtcgaatg ggtgcccgaa ctgtgatggc
     1081 ggcttcgaac ggcggcccgt ccggaccgcg cgcgtgggtc gaggcgcgac gccttcccgc
     1141 cctcgacgaa cgtggtccac aagccggtcg accccacggc tcacgaggca ctggtcgcct
     1201 tgatcggcgg gcggtcaccc tcgcagtgct gacaccgtgc gctgcgcaac cgaagtgagg
     1261 ctgcggaacg actcggtgcg ccgccattct cgggccgtag gtttccaccg tggtcgggct
     1321 gtcgggcccc gaggcgtcgg tctctcggag atccgggggg ccgagtcgcg tctcggagta
     1381 gccacctcag gccatctgct cgcgacatcc atcgacccga acgcgggctt gtgcggcttc
     1441 ccgccgctgc aaccgtgccg gtattccaga ggaggaatct gtggctcgac gaggccaacg
     1501 ccagactggc actcgccgta ccgccctacg gaaccagcga cgcgtcaacg accgcgacga
     1561 cgtcatctcg gtgcttgccc gcgccgtccg tgaggtggag gcagccgccc agcggggctc
     1621 cgtgacgccc gcggtgcgca cgaggttcca ggtggtcgcc ctgctgttgc gcgaggagca
     1681 cgccaggctt cgggcggatg cgaccagcag cgatacccac cgggccgagc agctcaagcg
     1741 cctcgacggg atcgcgacga tcctggcgag aaccgctgta cgcgaccccg cgctcctcgc
     1801 gctccttgcc gatgacgccg tcgtctccga cgcggccagg tcgctcaagc g
//
LOCUS       DASYLX010000688        10697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_696550,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000688 DASYLX010000000
VERSION     DASYLX010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696550"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGP27_16210"
     CDS             complement(<1..374)
                     /locus_tag="VGP27_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7852878.1"
                     /translation="MWHQRADVIVVGTGVAGLVAALAAHRRGRKVVVLSKAADTATFY
                     AQGGIAVVLPDTDDSIDAHVADTIAAGAGLCDPDAVRSIVSDGYRAVRELVDVGAQFD
                     ETETGRWSLTREGGHSRRRIIHS"
     gene            complement(374..1393)
                     /gene="nadA"
                     /locus_tag="VGP27_16215"
     CDS             complement(374..1393)
                     /gene="nadA"
                     /locus_tag="VGP27_16215"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV7852879.1"
                     /translation="MTVLDDRIINGPTGYSGVDGDEEWAAEVRRLANLRGATLLAHNY
                     QLPAIQDVADHVGDSLALSRIAAAAPEETIVFCGVHFMAETAKILSPDKTVLIPDQRA
                     GCSLADSITADELRAWKAEYPGAVVVSYVNTTAAVKAETDICCTSSNAVEVVASIPED
                     RDVLFCPDQFLGAHVRRVTGRENIHVWAGECHVHAGINGDELADKALAHPDAELYVHP
                     ECGCATSALYLAGEGAFPEDRVKILSTGGMLDAARETRARQVLVATEVGMLHQLRRAA
                     PEVDFRAVNDRASCKFMKMITPAALLRCLIEEADEVDVDPEVAARGRASVVRMIEIGT
                     PGGGE"
     gene            1440..2132
                     /locus_tag="VGP27_16220"
     CDS             1440..2132
                     /locus_tag="VGP27_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7852880.1"
                     /translation="MANTSTEHEVLAVVFQVRRLSTRNPQLSVLLWERAKEPQRGAWS
                     LPGGQLRHDEDMTSSVRRQLAEKVDLRELAHLEQLAVFSDPHRVPGARTIASTFLGLV
                     PSPATPELPPDTRWHPVNALPPMAFDHGTMIEYARTRLVAKMSYTNIGFALAPREFAL
                     STLRDIYGATLGHQVDATNLQRVLARRGVISPTGTTARSGRSGGRPAALYRFTESAYR
                     VTDEFAALRPPG"
     gene            2200..3564
                     /locus_tag="VGP27_16225"
     CDS             2200..3564
                     /locus_tag="VGP27_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV7852881.1"
                     /translation="MDLGSAAPAGSSSAPRVRTGAEWIGSVPHEDIVRGARPQLPRDD
                     PFYDAPQGFQHAAPGTVLRSRDVEVAFLGLIPQKFTATQLLYRSTDMNGEPEATVTTV
                     IAPAERAAGQVCPVLSYQCAIDAVTSRCFPSYALRRRALAPGALAQFEFFLIAAALAE
                     GWAVSVPDHEGAKGAWGTPYEPGYRILDGLRAALSTEKLGLSDAARIGLWGYSGGGLA
                     TSWAAEMAGSYAPELNIVGAVLGSPVADLGHAFRRLNGGVFSGLPAMVVAALTHAYPD
                     LDRVIQEHATDTGKAMLLRAEHMTTAHAVLRLVGMDMGKLVDRPLNEILDMPEVQKVF
                     DSIKLGTAVPSVPVLIVQAVHDPIVAVEDIDELIDIYESGGASVTYHRDLFSEHLSLH
                     PLSAPMALRWLTDRFAGKPVSENIVRTKWPTMLNPMTYVGMARLVRIVAKVVTGRTVE
                     RRPL"
     gene            complement(3530..4159)
                     /locus_tag="VGP27_16230"
     CDS             complement(3530..4159)
                     /locus_tag="VGP27_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2567 domain-containing protein"
                     /protein_id="HEV7852882.1"
                     /translation="MNEVAAPRISHERAAVTVVVGLAVAGAVIGALWAWIAPPVHGVI
                     ALTKSGDRVHAALGSESDNFFIAAVLLVGMAVVLAVVTAVLVWQWRPHRGPVLCAALG
                     LGSAAAFGVAAGVGALIVRARYDVIDIAGAAISPEHRVVYVTEAPPVFFSHSAWLIAA
                     SVLFPVALAMLAYALIAVSTSRDDLGGWPPEEPILPAPIRVDGVLPSAR"
     gene            complement(4156..4356)
                     /locus_tag="VGP27_16235"
     CDS             complement(4156..4356)
                     /locus_tag="VGP27_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852883.1"
                     /translation="MGPAKFNVYTGEPAGGAVPTAAQLGLEPPRFCAECGRRMVVQVR
                     PDGWTAKCSRHGTQNSKDLDKR"
     gene            complement(4371..5366)
                     /gene="bioB"
                     /locus_tag="VGP27_16240"
     CDS             complement(4371..5366)
                     /gene="bioB"
                     /locus_tag="VGP27_16240"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEV7852884.1"
                     /translation="MTDILTEAREQVLERGNGLTQDQVLTVLQLPDDRLDELLELAHD
                     VRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTA
                     KSGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQEQVERLSAMG
                     VHRYNHNLETAKSFFPEVVTTHSWEERWETLSMVRDAGMEVCCGGILGMGETLEQRAE
                     FAANLAELDPHEVPLNFLNPRPGTPFGDLDVLPASEALKAVAAFRLALPRTMLRFAGG
                     REITLGDLGAKQGILGGINAVIVGNYLTTLGRPAEADLELLDDLQMPIKALNATI"
     gene            5492..6079
                     /locus_tag="VGP27_16245"
     CDS             5492..6079
                     /locus_tag="VGP27_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV7852885.1"
                     /translation="MQLHKRDVVEAATAILDTYGIADLTMRRLGRELNVSAGALYWHF
                     ANKQELLGAIADRILEPVGDVSGDWRNRVVEVCGRLRDALLSHTDGAELVSASFAAGQ
                     SWAMKQIVARLGEAATDAGVADAHAALAARTVVYYVLGFTADEQSRLQWDAAGADLPD
                     EQSVLTDEAGDRFGFGLALLVDGIAARRAVVGGPD"
     gene            6090..6425
                     /locus_tag="VGP27_16250"
     CDS             6090..6425
                     /locus_tag="VGP27_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV7852886.1"
                     /translation="MITVADVRRITSTLPRSYEVVVGDRIKFRIGRIVYLAFSRDETV
                     MGFAFPKEERAGLVASEPDKFMLPRPSDMRYNWVHVYLDAVDAVELREIVVDAWRMCV
                     PKSVAATIS"
     gene            complement(6422..6958)
                     /locus_tag="VGP27_16255"
     CDS             complement(6422..6958)
                     /locus_tag="VGP27_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEV7852887.1"
                     /translation="MVHSVELLFDAETESAVRRLWDDLTDAGVRSLASSTSPTNRPHT
                     TLAVAEQMDDAVNNALRPLLAELPLACTLGAPMLFGRGPFTLVRLVIPSVQLLALQAE
                     VHAVCLPYMSPGGLPHANPGHWTPHVTLARRVPQDRLTDALSLRRMSHDQRATAVALR
                     HWDGNKRVEYPITERSID"
     gene            complement(6959..7639)
                     /gene="bioD"
                     /locus_tag="VGP27_16260"
     CDS             complement(6959..7639)
                     /gene="bioD"
                     /locus_tag="VGP27_16260"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEV7852888.1"
                     /translation="MSVLVVTGTDTGVGKTVATAALACHARLAGIDVAVCKPVQTGYA
                     DGDDDLAEIGRLSGVTELHGLARLPEPLAPAAAAERAGTRLPTRRDLASMVVSADRPD
                     RLTLVEGAGGLLVEIGEGGTTLRGLAVDLAAPVLVVARAGLGTLNHVALTMEALASHN
                     VWCAGLVIGAWPQSQGVIESGNREALSTMAPLRAVLPDGAGRYLPAQFESMSSEAFDT
                     QWIRSLVG"
     gene            complement(7636..8808)
                     /locus_tag="VGP27_16265"
     CDS             complement(7636..8808)
                     /locus_tag="VGP27_16265"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEV7852889.1"
                     /translation="MVGSARSTVTRVGLSPLAWLDEVERDRRAAGLRRSLRTRPPVGA
                     ELDLASNDYLGLSQHPDVIDGGIGALRTWGAGSSGSRLVTGNTELHEGFEAALADFVG
                     TESALVFSSGYTANLGAVVALSGPGSLLVSDALTHASLVDACRLSRARVAVTPHRDVA
                     AVEHALSSRDEDRAVVITDSVFSADGDLAPLRELHDVCRRHGALLIVDEAHGLGVRGT
                     GGRGLLEEVGLAGVPDVVMTVTMSKALGSQGGAVLGPAAVRAHLIDAARPFIFDTGLA
                     PAAVGAAWSALRVLTAEPWRARDVLRYAAELAKACDVSDQPESAVVSVVLGAPEVALA
                     AATACLERGVRVGCFRPPTVPAGTSRLRLTARASLSENEMALARTVLTEVLSTARQ"
     gene            complement(8844..10136)
                     /locus_tag="VGP27_16270"
     CDS             complement(8844..10136)
                     /locus_tag="VGP27_16270"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEV7852890.1"
                     /translation="MSALTPAEISAIDAAHVWHPYSTIGAEAMPPVVAVGASRAWLTV
                     IHDGKRVDVLDAMSSWWTAIHGHGHPALDYAIASQLARMNHVMFGGLTHEPAARLAQL
                     LVEITPDGLDTVFFSDSGSVSVEVAVKMALQYWRSLGRRNKHRLMTWRGGYHGDTFTP
                     MSVCDPDGGMHSLWTDVLAHQVFAPQVPTEYDPAYSAAFEAQLAEHADELAAVIVEPV
                     VQGAGGMRFHDPRYVSDLRDICTRHQVLLIFDEIATGFGRTGALFAAEHAGVDPDIMC
                     VGKALTGGYITLAATLCTTDIAHTISRHEPGALMHGPTFMANALACAVSVASVELLLG
                     QDWRARIAEIEAGLREGLEPARLLPGVADVRVLGAIGVIEMAEPVDMPVATAAALEHG
                     IWLRPFRNLVYAMPPYICTREEIGQITSAMVAVACALT"
     gene            10245..>10697
                     /locus_tag="VGP27_16275"
     CDS             10245..>10697
                     /locus_tag="VGP27_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7852891.1"
                     /translation="MMTLAPPRPAVVNASAAGKGASAADRKSVFYRHDLDGLRGIAIA
                     LVAVFHVWFGRVSGGVDVFLALSGFFFGGRLLRAALTPGASLSPVPEVTRLVRRLLPA
                     LVVVLAAAAVLTILIQPETRWETFADQSLASLAYFQNWELANTASDYLR"
BASE COUNT         1773 a         3690 c         3515 g         1719 t
ORIGIN      
        1 gaatggatga tgcggcgccg cgagtggccg ccctcgcgcg tcagcgacca gcggccggtc
       61 tcggtctcgt cgaattgcgc tccgacatcg accaattcac gaaccgcgcg gtaaccgtcc
      121 gacacgatgg agcgcaccgc atcgggatcg catagaccgg cgcctgcggc aatcgtgtcg
      181 gcgacatggg catcgatcga gtcgtcggtg tccggcagca ccaccgcgat gccaccctgc
      241 gcgtagaacg tcgcggtgtc ggcggccttg ctgagcacga ccaccttgcg gccgcgacgg
      301 tgtgcggcca gcgcggcgac caatcccgcg accccggttc cgacgacgat gacgtcggcc
      361 cgctggtgcc acatcattca ccgccgccgg gtgtgccgat ctcgatcatc cgcacaacgc
      421 tggccctgcc gcgggcggcg acctcgggat cgacgtcgac ctcgtcggct tcctcgatca
      481 ggcagcgcag cagtgcggcc ggggtgatca tcttcatgaa tttgcaggac gcacggtcgt
      541 tgacggcgcg gaagtcgact tccggtgcgg cccggcgcaa ctggtgcagc atgccgacct
      601 cggtggcgac cagcacctga cgggcacggg tctcgcgagc cgcgtccagc atgcccccgg
      661 tggacagtat cttcacccga tcctcgggga aagcgccctc gcctgccagg tacaaagccg
      721 atgtggcaca gccgcattcg ggatgcacgt agagctcggc gtcgggatgt gcgagcgcct
      781 tgtctgccag ttcgtcgcca ttgatgccgg catgcacatg gcattcgccc gcccagacgt
      841 ggatgttctc gcgaccggtg acccgtcgga cgtgcgcgcc gaggaactgg tccgggcaga
      901 acaggacgtc gcggtcctcg gggatggagg ccaccacttc gacggcgttg gacgacgtgc
      961 agcagatgtc ggtttccgcc ttcaccgctg cggtggtgtt gacgtaggac accacgaccg
     1021 cgccgggata ctcggccttc cacgcgcgca gctcgtcggc ggtgatcgag tcggccagtg
     1081 aacagccggc gcgctgatcg gggatcagca ccgtcttgtc cggagacagg atcttcgcgg
     1141 tctcggccat gaagtgaacg ccgcagaaga cgatggtttc ctcgggggcg gccgcggcga
     1201 tccgcgacaa cgccagcgag tcgccgacgt ggtcggcgac gtcctgaatg gcaggcagct
     1261 ggtagttgtg cgccagcagc gtcgcgccac gcaggttggc caggcgtcgc acctcggcgg
     1321 cccattcctc gtcgccgtcg acgccggaat agccggtcgg accgttgatg atccggtcgt
     1381 ccaaaaccgt catggccgcc tcctcgcaga ccgtaggttt tcgacttaca atcgaaaaca
     1441 tggccaatac tagcactgag catgaagtgc ttgccgtcgt ctttcaggtt cggcggctca
     1501 gcacccggaa cccacagctc agcgtgctgc tgtgggaacg ggcgaaagaa ccgcagcgtg
     1561 gggcctggtc gctgcccggt gggcagctgc gtcacgacga ggacatgacc agttcagttc
     1621 ggcgacagct cgccgagaag gtcgacttac gtgagttagc tcaccttgag cagctggccg
     1681 tgttttccga cccgcaccgc gttcccggcg cccgcaccat cgcgtcgacg tttctcggcc
     1741 tggttccctc ccccgccacc cctgagttgc cgcccgacac ccgctggcat ccggtcaacg
     1801 cgctgccgcc gatggcgttc gaccacggaa cgatgatcga atacgcgcgc acccgactgg
     1861 tggcgaagat gtcatacacg aatatcgggt tcgccctggc gccaagggaa ttcgccctgt
     1921 cgacattgcg ggatatctac ggtgcgacgc tgggtcatca agtcgacgcc accaacctgc
     1981 agcgcgtgct cgcacgacgc ggcgtcatct ccccgaccgg cacgaccgcc cgatcggggc
     2041 gcagcggcgg tcggcccgcg gccctgtacc gcttcaccga gtcggcctac cgcgtcaccg
     2101 acgagttcgc ggcgctccgg ccgcccggat gaggcgtact gaacggctgt tcacggacgc
     2161 gactggattc ttaatgtctt cttaagccac aatggccgga tggacttggg cagcgccgca
     2221 ccggccggat cttcttctgc tcctcgcgtg cgcaccggtg ccgaatggat tggttccgta
     2281 ccgcacgaag acatcgtgcg gggtgcccgt ccgcaactgc caagggatga cccgttctac
     2341 gacgccccgc agggcttcca gcacgccgcg ccgggaaccg tcctgcgcag ccgtgacgtc
     2401 gaagttgcct tcctcgggct gatcccgcag aagttcaccg ccacccagct gctgtaccgc
     2461 agcaccgaca tgaacggcga gccggaggcc accgtcacca cggtcatcgc ccccgccgag
     2521 cgcgctgccg ggcaggtctg ccccgtgctg tcgtatcagt gcgccatcga cgccgtcacg
     2581 tcgcgctgct tcccctccta tgcgttgcgc aggcgggcgc tggcgccggg agcgctcgcc
     2641 cagttcgagt ttttcctcat cgccgcggcg ctcgccgagg gctgggcggt gtcggtgccc
     2701 gaccacgagg gcgccaaggg ggcctggggc acaccgtatg agccgggata tcggatcctc
     2761 gacgggctgc gcgcggcgtt gagcacggag aagctgggcc tgtcggacgc tgcgcggatc
     2821 gggctgtggg gctactcggg cggcggcctc gcgacttcgt gggccgccga gatggccggg
     2881 tcgtatgcgc ccgagctgaa catcgtcggc gctgtgctcg gatctcccgt cgccgatctg
     2941 ggccatgcct tccgtcgtct caatggcggc gtcttctccg gactgcccgc catggtggtc
     3001 gcggcgctga cacacgcgta tccggacctc gaccgcgtca ttcaggagca cgcgaccgac
     3061 accggtaagg cgatgctgct gcgggccgag catatgacca ccgcacatgc ggtgctgcgg
     3121 ctcgtcggca tggacatggg taagttggtc gaccggccgt tgaacgagat cctcgacatg
     3181 cccgaggtgc agaaggtatt cgacagcatc aagctgggca cggctgtgcc gagcgtgccg
     3241 gtgctgatcg tgcaggcggt gcacgacccg atcgttgccg tcgaggacat cgacgagctg
     3301 atcgacatct acgaatccgg cggcgcctcg gtgacctacc accgggatct cttcagcgag
     3361 cacctatcgc tgcacccgtt gtcggcgccg atggccctgc gctggctgac cgaccggttc
     3421 gcgggaaagc cggtgagtga gaacatcgtc cggacgaagt ggccaacgat gctcaatccg
     3481 atgacctacg taggtatggc caggctggtg cggatcgtcg cgaaagtcgt taccgggcgg
     3541 acggtagaac gccgtccact ctgatcgggg ccggcaggat cggctcctcc ggaggccagc
     3601 cgccgagatc atcgcgtgac gtcgacaccg cgatgagggc gtaggccagc atcgccagcg
     3661 ccacgggaaa cagcaccgat gcggcgatca accacgccga gtgcgagaag aacaccggcg
     3721 gcgcctcggt cacgtagacc acgcgatgct caggcgagat cgccgcaccg gcgatgtcga
     3781 tgacgtcgta gcgtgcccgc acgatcaacg cacccacgcc cgcggccaca ccgaacgcgg
     3841 ccgcagaacc gagtcccagc gcggcgcaca acaccggacc gcggtggggc cgccactgcc
     3901 acaccaacac cgcggtcacc acggccagca ccaccgccat gccgacgagc aacaccgccg
     3961 cgatgaagaa gttgtccgat tcgctcccaa gagcggcgtg cacgcgatcg ccgcttttgg
     4021 tcagtgcgat gacgccgtgg accggcggtg cgatccacgc ccacagcgca ccgatcacgg
     4081 caccggccac cgcaagaccg acgaccactg tcacggcggc acgctcatgt gagatccgcg
     4141 gtgctgcaac ctcattcatc gcttgtcaag gtccttcgag ttctgggtgc cgtgccggga
     4201 gcatttggcc gtccacccgt cggggcgcac ctgcaccacc atccgcctgc cgcattccgc
     4261 gcagaaccgt ggtggctcga gtccgagttg ggccgcagtg ggcacggcgc cgcctgcggg
     4321 ctcgccagtg tagacgttga atttggcggg tcccatcaga gaagccacca ctagatcgtg
     4381 gcgttgagcg cctttatcgg catctggaga tcgtcgagca gctccaggtc cgcctcggcc
     4441 ggacggccca gcgtcgtgag gtagttgccg acgatgacgg cgttgatgcc gcccaggatg
     4501 ccctgcttgg cgccgaggtc gccaagagtg atctcgcggc cgcccgcgaa tcgcaacatg
     4561 gtgcgcggca gcgccagccg gaacgccgcg accgccttca gcgcctccga cgcgggcagc
     4621 acgtccaggt cgccgaaggg tgtgccgggg cgcgggttga ggaagttcag cggaacctcg
     4681 tgggggtcga gttcggccag attcgccgcg aactcggcac gctgctccag cgtctcaccc
     4741 atgccgagga tgccgccgca gcagacctcc atgccggcat cgcggaccat cgacagagtc
     4801 tcccagcgct cctcccagga gtgagtggtc accacctcgg ggaagaagga cttggcggtc
     4861 tccaggttgt ggttgtagcg gtgcacgccc atcgccgaca gccgctcgac ctgttcctgc
     4921 gtcagcatgc ccagcgagca ggcgatctgg atgtcgacct cgttgcggat cgcctcgatg
     4981 cccgcggcca cctgcgcgag caggcgttcg tcggggccgc gcacagccgc cacgatgcag
     5041 aactccgtcg caccggactt ggccgtctgc ttggcggctt cgacgaggct cggaatgtcg
     5101 agccatgcgc tgcgcaccgg cgaggcgaaa agcccggact gcgaacagaa atggcagtcc
     5161 tctgggcagc ccccggtctt gaggctgatg atgccctcga cctcgacctc cgggccgcac
     5221 cagcgcatcc gcacgtcgtg ggccagttcg agcaactcgt cgagccggtc gtcgggcagc
     5281 tgcagcacgg tcagcacctg gtcctgcgtc aggccgttac ctcgctcgag cacctgctcg
     5341 cgggcctcgg tcaggatgtc cgtcaaacca ctgctcccat cacaagcttg aacccctcgg
     5401 tcgagccgag aattaaacac ctcggcggtg ccgagaattg aacacctcgg ccgagccgag
     5461 aattgaacac cgttcaggtt atggtagcga ggtgcagctg cacaaacgcg acgtggtcga
     5521 ggcggcgacg gcgatcctgg acacctatgg gatcgccgac ctgacgatgc gcaggctcgg
     5581 gcgggagttg aatgtctccg ccggtgccct gtactggcat ttcgcgaaca agcaggagct
     5641 tctcggcgcg atcgccgacc gaatcctcga gcccgtcggc gacgtctccg gcgattggcg
     5701 aaatcgcgtg gtggaagtct gcggccgtct gcgggatgcc ctgctgtcgc acaccgacgg
     5761 tgcggagctg gtgtcggcca gcttcgccgc aggccagtcc tgggcgatga agcagatcgt
     5821 ggcgcgcttg ggcgaggccg ccaccgatgc gggcgtcgcg gacgcgcatg cggcgttggc
     5881 ggcccgaacc gtcgtctact acgtgctggg gttcaccgct gacgagcagt cccggcttca
     5941 atgggacgcc gcaggtgccg atctgccgga cgagcagtcg gtgctgacgg atgaggcagg
     6001 cgatcgcttc ggattcgggc tcgcgctgct tgtcgacggc atcgccgcgc ggcgcgccgt
     6061 tgtcggcggc cccgactaac gtcacaggcg tgatcaccgt cgctgatgtg cggcgcataa
     6121 cctcgaccct gccgcggtcc tacgaggtgg tggtcggcga ccggatcaag tttcgtatcg
     6181 gacggatcgt ctacctcgcg ttctcccggg acgagacggt gatgggtttc gccttcccca
     6241 aggaggagcg tgccggactc gtcgcctccg aacccgacaa gttcatgctg ccgaggccgt
     6301 ccgacatgcg gtacaactgg gttcacgttt acctcgacgc cgtcgacgcc gttgaattac
     6361 gcgagatcgt tgtcgacgca tggcggatgt gcgtgccgaa aagcgttgcg gcaactatct
     6421 cttagtcgat cgaccgttcg gtgatcggat attccacgcg cttgttgccg tcccagtgcc
     6481 gcagtgccac cgctgttgct cgctgatcgt gcgacatccg gcgaagcgac agcgcatcgg
     6541 tcagcctgtc ttgcggaacc cttcgcgcaa gcgtgacgtg aggcgtccag tgtccgggat
     6601 tcgcgtgcgg gaggccgccg ggcgacatgt acggcaggca cactgcgtgc acctcggctt
     6661 gcagcgccaa cagctgcacg gacgggatca ccagacgcac cagagtgaac ggcccgcgtc
     6721 cgaacagcat cggcgccccc agcgtgcagg ccaacggcag ctccgccagc agcggacgca
     6781 gcgcgttgtt gaccgcgtca tccatctgct cggcgaccgc cagcgtcgta tgtggccgat
     6841 tggtcggcga cgtcgagctc gccaggcttc gtacacctgc atcggtcagg tcgtcccaga
     6901 gtcgccggac cgcggactcg gtctcggcgt cgaagagcag ctcgacggaa tggaccattc
     6961 agccgaccag agacctgatc cactgcgtgt caaacgcctc tgagctcatc gactcgaact
     7021 gcgcaggcag gtaacggcct gcgccgtcgg gcaacacggc ccgtagcggc gccatcgtcg
     7081 agagcgcctc gcggttgccc gattcgatga ccccttggct ctgcggccat gctccgataa
     7141 ccaggccggc acaccaaacg ttatgcgaag caagggcttc catcgtcaat gcaacatggt
     7201 tcaacgtgcc gaggcccgct ctggcgacga cgagcaccgg agccgcgagg tcgaccgcga
     7261 ggccacgaag cgtcgtcccg ccctcaccga tctccacgag caggcctcct gcaccctcga
     7321 ccagggtcag ccggtcaggc cggtcggccg aaacgaccat cgacgccaaa tctctgcgtg
     7381 tcggcaggcg cgttcctgcc cgctccgccg ccgccgcagg agcaagaggc tcgggaagcc
     7441 ttgcaaggcc gtgtaattca gtgacaccgg acagccgccc gatctcggcc aggtcgtcat
     7501 cgccgtcggc gtagccggtt tgcaccggct tgcacaccgc gacgtcgata ccggccagcc
     7561 gcgcatgaca cgccagcgcg gcggttgcca ccgtcttgcc gacgccggtg tcggtgcctg
     7621 tgacgacgag aacgctcact ggcgagccgt cgacaacact tccgtgagca ccgtgcgcgc
     7681 cagtgccatc tcgttctcgg acagcgatgc ccgcgcggtc aggcgcagcc gcgacgtgcc
     7741 cgcgggcact gtgggcggac ggaaacatcc cacccgcaca cccctttcga ggcaagcggt
     7801 cgcggcggcg agcgcaacct cgggcgcacc gagaaccaca gacaccaccg ccgattccgg
     7861 ttggtccgaa acgtcgcacg ccttggccag ttcggcggcg tagcgcaaca cgtcgcgggc
     7921 ccgccacggt tcggctgtca acacccgcag ggccgaccac gctgcgccaa cggccgccgg
     7981 cgccagcccg gtgtcgaaga tgaagggcct ggccgcgtcg ataaggtgag cgcgcaccgc
     8041 agccggtccg agcaccgcac cgccctggct gccgagcgcc ttggacatcg tcacggtcat
     8101 cacgacgtcc ggcacaccgg ccaggccgac ttcctccagc aagccccggc cgccggtccc
     8161 ccgcacgccg agcccgtgcg cctcgtcgac gatcagcagc gcgccgtggc ggcggcagac
     8221 atcatgcaat tcgcgcagcg gagcgaggtc accgtcggcg ctgaagaccg aatcggtgat
     8281 caccacggcg cgatcctcgt cgcgcgacga cagcgcgtgc tccaccgcgg ccacatcgcg
     8341 atgcggcgtc accgccaccc gcgccctcga cagacggcag gcgtcgacca gtgacgcatg
     8401 ggtcagcgca tcggacacga gaagcgagcc cggcccggac aatgcgacca ccgcaccgag
     8461 attggcggta tagcccgacg agaacaccag cgccgactcg gtgccgacga agtcggcaag
     8521 cgcggcctcg aagccctcat gcagttcggt gtttccggtc accagccgtg aacccgacga
     8581 tcccgcgccc caggtgcgca gcgcgccaat gcctccgtcg atcacatcgg gatgctgcga
     8641 cagacccagg tagtcgttgg aggccaggtc gagttcagcg cctaccggcg gcctcgtgcg
     8701 cagcgaccgg cgcagtccgg ccgcccgccg gtcacgctcg acctcgtcga gccaggccag
     8761 tggggaaagg cctacccgcg tcacggtgct cctagctgag ccgacaattg aacacgtcgg
     8821 cggagccgac aattgaacag tgttcaggtt agtgcacagg cgacggcgac catcgcggag
     8881 gtgatctgcc cgatctcctc ccgggtgcag atgtagggcg gcatggcata caccaggttg
     8941 cggaaggggc gcagccagat gccgtgctcc aacgccgccg cggtggccac cggcatgtcg
     9001 accggttccg ccatctcgat gacaccgatg gcgccgagca cacggacgtc ggccacgcca
     9061 ggcagcaacc gggccggctc gaggccctcg cgcaggccgg cctcgatctc ggcgattcgc
     9121 gcccgccaat cctgaccgag cagcagctca accgatgcca ccgacaccgc gcatgcgagc
     9181 gcattggcca tgaacgtcgg gccgtgcatc agcgcacccg gctcgtgcct gctgatcgtg
     9241 tgtgcgatgt ccgtcgtgca cagcgtggcg gccagggtga tgtatccacc tgtgagcgcc
     9301 ttgccgacac acatgatgtc ggggtccacc ccggcgtgct ccgcggcgaa cagcgcgccg
     9361 gtgcggccga aaccggtggc gatctcgtcg aagatcagca gcacctggtg gcgcgtgcag
     9421 atgtcccgca ggtcgctcac ataccgcggg tcatggaatc gcatgccacc ggcgccctgc
     9481 acgaccggtt cgacgatgac ggccgcgagt tcgtcggcat gttcggccag ttgcgcctcg
     9541 aaggccgcgc tgtaggccgg gtcgtattcg gtgggcacct gcggggcgaa gacctgatgc
     9601 gccaggacgt cggtccacaa cgagtgcatg ccgccgtccg ggtcgcacac gctcatcggc
     9661 gtgaaggtgt caccgtgata gcccccgcgc cacgtcatca gcctgtgctt gttccgacga
     9721 cccagactgc gccagtactg cagcgccatc ttcaccgcga cctcgaccga gaccgatccc
     9781 gagtcgctga agaagacggt gtccagcccg tcgggcgtga tctccaccag cagctgggcg
     9841 agccgagccg ccggttcgtg ggtcagcccg ccgaacatga cgtggttcat cctggccagc
     9901 tgggaggcga tggcgtagtc gagagccggg tggccgtgcc cgtggatcgc ggtccaccac
     9961 gagctcatcg cgtcgagcac gtcgacgcgc ttgccgtcgt gtatcaccgt cagccacgct
    10021 ctgctcgccc ctacggcgac cacgggcggc atcgcctctg cgccgatcgt gctgtaggga
    10081 tgccagacgt gggcggcgtc gattgcgctg atttcagcgg gcgtcaacgc ggacatcgga
    10141 aacgagccta accctttgcc ggccgatacc cgttgccacc gaagcgggcg gcggcacggc
    10201 ggacatgagt cacacagcga ttttcggtag attgacccgc gaccatgatg accctcgccc
    10261 ctccccggcc ggcggtggtc aacgcgagcg ctgccggcaa gggcgcgtcc gcggctgacc
    10321 gcaagtctgt cttctaccgc cacgatctgg acggccttcg cggtatcgcg atcgccctcg
    10381 tcgcagtgtt ccacgtgtgg ttcggtcgcg tttcgggcgg cgtggacgtt tttctcgcct
    10441 tgtcgggatt cttcttcggc ggccgcctgc tgcgggccgc gctgacgccc ggcgcatcgc
    10501 tttcacccgt tcccgaagtg acccggctgg ttcgccggct gctgcccgca cttgtcgtgg
    10561 tgctggccgc ggcggccgtg ctgacgatcc tgatccaacc ggagacgcgt tgggagacgt
    10621 ttgctgacca gagcctggcc agcctcgcct acttccagaa ctgggaactg gcgaacactg
    10681 catcggacta cctccgc
//
LOCUS       DASYLX010000689         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1792994,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000689 DASYLX010000000
VERSION     DASYLX010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792994"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VGP27_16280"
     CDS             complement(<1..981)
                     /locus_tag="VGP27_16280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV7852892.1"
                     /translation="MTAPSALDTDKPVLQARDIVKTYGGVTALDGVSFDLRPGEVHCL
                     AGENGCGKSTLIKIISGAERPDSGEIIVDDVAHRAMNATAAITSGIQVIYQDFSLFGN
                     LTVAENIALTSAVAERRRLYSPATAQKIARRIVDDLRLNLDLDADVETLPVADRQLTA
                     ICRAVVHEARVIIMDEPTTALTHSEVDRLFELVETLRARGVGLVFVSHKLDEVLRVAQ
                     RVTILRSGRKVVTGPAADFTPQSIGEFMTGRKISEERIVSDLPADVPVVLDVEALTRT
                     GAFEGVSFAVREGEILGVTGLLGSGRTEIAEALFGVRPASSGTVKLDGKPV"
     gene            complement(1048..2094)
                     /locus_tag="VGP27_16285"
     CDS             complement(1048..2094)
                     /locus_tag="VGP27_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7852893.1"
                     /translation="MQHRRMALLGAVGLTMTLIAGCGSVGGGGGSSSSAAAGASQEPK
                     DMTMVNVVKIKGISWFDRMAVGDENFAKRTGVDTRQEGADDTSPEKQIQIIQDLIPQK
                     PTAITVVPNSPEALQNVLGQARGQGIKVVSHEATGIQNADIDIEAFDNAAYGRQIMQN
                     LAQCMGQQGKYVQFVGGLTAKTHMQWVGAAHDMQVSQFPGMTRVEDPIESTDNEQAAY
                     EKAKEVLAKYPDIKGFQGSAGNDVPGIARAVQEAGLQNKVCVMGTSIPSAASKYLADG
                     SIDKIFFWDPALAGEAQLQIALMLAQGKTIEAGTNLNIPGYEKLTKLDGYDNVYVGDA
                     ALEADAKTMSQYNF"
     gene            2086..3069
                     /locus_tag="VGP27_16290"
     CDS             2086..3069
                     /locus_tag="VGP27_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV7852894.1"
                     /translation="MLHVSLPCEPLHFTRTAIPACVTLNANCLIIKQLNARNRAYRAL
                     VCTKMSVGVCLRVLRPNAGGCIVGGGPVSMSRRADILNHLMDRGFQSVNALAQGFAVD
                     ASTIRRDLDKLEAQGLVQRTHGGALPVKPADAPAGGHGRHRAEMAAIGAAMAERVLEG
                     QTILLDSGETTLEVARHLHHSRLTVVTHDLRVGLEVATKRSINLVFIGGELLPSGFGM
                     WGPSSLQQVENLRVNVAIFGAGTVMDDGIYSTSSYEIELKRKMRSIASEAFFVADSSK
                     FGREALFKVFGFEHFTAGITDAMLDPIRAAQLPIPVIRVAVGTQDEDRHGR"
     gene            complement(3085..>3566)
                     /locus_tag="VGP27_16295"
     CDS             complement(3085..>3566)
                     /locus_tag="VGP27_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852895.1"
                     /translation="EWFAGRLPDWFDGDPAVVVDREEITVIGRLPEPEGEESEARASG
                     RASRFREETRAERMRIADEAQDKYGRKVSWGVEVGSGEAAERILFTHIAVPVMTRLRQ
                     PERQVLDTLVDAGVARSRSDALAWSVKLVGEHADEWLTKLRDAMKNVDDLRSEGPQA"
BASE COUNT          570 a         1134 c         1161 g          701 t
ORIGIN      
        1 taccggcttg ccgtcgagtt tgacggtgcc gctggaggcg ggtcggacgc cgaacagagc
       61 ctcagcgatc tcggtgcgcc ccgagccgag caggcctgtc acgccgagga tctcgccttc
      121 ccggacggcg aagctgacgc cttcgaaggc gccggttcgg gtcagtgcct cgacgtcgag
      181 cacgacgggg acgtccgccg gcaggtcgct gacgatccgt tcctcgctga tcttgcgacc
      241 ggtcataaat tcgccgatcg attggggggt gaagtcggcc gcaggcccgg tgaccacctt
      301 gcggccggat cgcaggatcg tcacccgctg cgcgacgcgc agaacctcat cgagcttgtg
      361 cgagacgaag accagcccga cgccgcgtgc ccgcagcgtc tcgacaagct cgaagaggcg
      421 gtcaacctcg ctgtgggtga gtgcggtggt gggctcgtcc atgatgatga cgcgggcttc
      481 gtgtaccacg gcgcggcaga tggcggtgag ttgccggtcc gcgacgggca gggtttcgac
      541 gtcagcgtcg aggtccaggt tcaggcgcag atcgtcaacg atgcggcgcg cgatcttctg
      601 cgcagtggcg ggcgagtaga gccttcggcg ttcggcgaca gcgcttgtca gagcgatgtt
      661 ctcagcgaca gtgaggttgc cgaacagcga gaagtcctgg tagatcacct ggatgccgct
      721 ggtgatcgcc gcggtggcgt tcatcgcgcg atgcgcgacg tcgtcgacga tgatctcacc
      781 ggagtccggc cgctccgccc cggagatgat cttgatcaga gtggatttgc cgcatccgtt
      841 ttcgcctgcg agacagtgca cttcgccggg acggaggtcg aaggaaacgc cgtccaaggc
      901 ggtgacgccg ccgtaggtct tgacgatgtc acgtgcctgc agcaccggct tgtctgtgtc
      961 caatgcgctc ggcgctgtca tggggaacgc tttccgggcc ggcccggcag gtcagcgcga
     1021 gccggcacct tgacctacgg tgcggactca gaagttgtac tgggacatgg ttttggcgtc
     1081 agcttccaac gccgcatcgc cgacgtagac gttgtcgtag ccgtctagct tggtcagctt
     1141 ctcgtagccc ggaatgttca gattggttcc ggcttcgatt gtcttgcctt gggcgagcat
     1201 caacgcgatc tgcaactgtg cttcaccggc cagcgccggg tcccagaaga agatcttgtc
     1261 gattgagccg tcggcgaggt acttcgaggc agcggacggg atcgacgtgc ccatcacaca
     1321 gaccttgttc tggaggccgg cttcctgcac agcgcgcgca atgcccggca cgtcgttgcc
     1381 ggccgacccc tggaagccct tgatatccgg gtatttcgcc agcacttcct tggccttctc
     1441 ataggcggcc tgctcgttgt cggtgctctc gatcgggtcc tcgacgcggg tcatgcccgg
     1501 aaattggctg acctgcatgt catgggcggc gccgacccac tgcatgtgcg tctttgcagt
     1561 caggcccccg acgaactgga cgtatttgcc ctgctggccc atgcattgcg cgaggttctg
     1621 catgatctgc ctgccgtagg cggcgttgtc gaaagcctcg atgtcgatgt cggcgttctg
     1681 aatgccggtg gcctcgtggg aaacgacctt gatgccctgg ccgcgggcct ggccgagaac
     1741 attttgcaga gcttccggag agttcggcac cacggtgatg gcagtgggtt tttgcgggat
     1801 cagatcctgg atgatctgga tctgcttctc cgggctggtg tcgtccgcgc cttcctggcg
     1861 ggtgtcgacc ccggtgcgct tcgcgaagtt ctcgtcgccg acggccatcc ggtcgaacca
     1921 ggagatgccc ttgatcttga cgacgttgac catcgtcatg tccttcggct cctgggaggc
     1981 cccggccgca gcgctgctgc tactgccgcc tccaccgccg accgatccgc agcccgcgat
     2041 cagcgtcatg gtcaagccca cggctcccag aagggccatc cgcctgtgct gcatgtgtca
     2101 ctcccttgtg aacccctgca cttcacccgg actgctatcc cggcttgcgt gacattaaac
     2161 gcgaactgcc tgataatcaa gcaattgaat gcgcgaaatc gtgcatacag agcgctcgtg
     2221 tgcacgaaaa tgagtgttgg tgtttgctta cgtgttctgc ggcccaatgc aggaggctgc
     2281 atcgtcggag ggggtccggt gtccatgtcg cgtcgcgcgg acatcctgaa ccatctgatg
     2341 gaccgcggtt ttcaaagcgt caacgcattg gcccaaggct ttgcggtcga cgcctcgacc
     2401 attcggcgcg atctggacaa gctcgaggcg caaggcctcg tccaacgcac gcacggcggc
     2461 gcgctgcctg tgaagcctgc cgatgcgccg gccggcggcc atggcaggca tcgcgcagag
     2521 atggcggcca tcggcgccgc gatggccgag cgggtgctgg aggggcagac gatactgctc
     2581 gacagcggcg agacgacgct cgaggttgct cgtcatctcc atcattcgcg actgaccgtc
     2641 gtcacccacg atctgcgtgt cggcctggag gttgccacca aacgatcgat caacctggtg
     2701 ttcatcggtg gggaattgct gccgtccggc ttcgggatgt ggggcccgtc gtccctccag
     2761 caagtggaga acctgcgggt caatgtggcc atcttcggcg caggcacggt catggacgac
     2821 ggcatatact ccacctcgag ttacgagatc gagctaaagc gcaagatgcg gtccatcgcg
     2881 agcgaggcgt tcttcgtcgc ggacagctcc aaattcgggc gcgaagcgct cttcaaagtc
     2941 ttcggcttcg agcacttcac tgccggcatc acggacgcca tgctcgatcc gatccgggcc
     3001 gcgcagcttc cgataccggt catccgggtc gccgtgggta cacaggatga agatcgacac
     3061 ggccgctgac ttttcgaaat cgactcaggc ctgcgggcct tctgacctga ggtcgtcgac
     3121 gtttttcatc gcgtcgcgca gcttggtcag ccactcatcg gcgtgttcgc cgaccagctt
     3181 gaccgaccag gccagcgcgt cggagcgcga ccgggcgacc ccggcgtcca cgagcgtgtc
     3241 gaggacctgg cgttccggct ggcgcagtcg cgtcatcacc ggaacggcga tgtgggtgaa
     3301 caggattcgc tcggccgcct ccccactgcc gacctcgacg ccccaggaca ccttgcggcc
     3361 gtacttgtcc tgcgcctcgt cggcgatccg catgcgctcc gcccgcgttt cctcgcggaa
     3421 tcgggacgcc cggcccgatg cacgggcttc gctctcttca ccttcgggct cgggtaaccg
     3481 cccgatcacg gtgatctctt cgcggtcgac gaccacggcg gggtcgccgt cgaaccagtc
     3541 gggcaggcgg cccgcgaacc actcgg
//
LOCUS       DASYLX010000690         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2998977,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000690 DASYLX010000000
VERSION     DASYLX010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998977"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="recR"
                     /locus_tag="VGP27_16300"
     CDS             <1..490
                     /gene="recR"
                     /locus_tag="VGP27_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV7852896.1"
                     /translation="LTAVLGKVRDGVKFCAVCGNVSDDDRCRICSDSRRDASLVCVVE
                     EPKDVQAVERTREFRGRYHVLGGALDPLSGVGPEQLRIRELLNRIGERVDGVDIAEVI
                     IATDPNTEGEATATYLVRMLRDIPGLTVTRIASGLPMGGDLEFADELTLGRALSGRRV
                     LT"
     gene            596..1486
                     /locus_tag="VGP27_16305"
     CDS             596..1486
                     /locus_tag="VGP27_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852897.1"
                     /translation="MAEVFIGSEELVAGRLTRHGLQRWYRPVYRGVYAPKDANLSLRD
                     RAIAAWLASGRKGVVAGVAASALHGAPWVDPDVPIELVGVKIRPQPGLVPRADRIADD
                     EITRAAGLPVTSRTRTAFDMGRHLERTDAVTRLDALMWNQEFSRADVRALGDRYPRAA
                     GLRRLLELLPLIDGGAESPPESRVRLWLSDAGFPRPETQIPVLQGSTAAVAFLDMGWR
                     DFMVAVEYDGDHHRKDRRQYVKDIARLRMLEALGWIVIRVNAEDKPSDVIARVEAALV
                     RRGCCIEIDELQRFTRTFAA"
     gene            complement(1493..>1820)
                     /locus_tag="VGP27_16310"
     CDS             complement(1493..>1820)
                     /locus_tag="VGP27_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV7852898.1"
                     /translation="GEVVSQPLPAGLTERLTGFENHRGGTVLGPDAQPLARVVSGAGN
                     REGDGVDGAVQGSVIATYLHGPCLARNPQLADHLLARVVGDLAPLDLPEVDKLRHERL
                     AAPRRV"
BASE COUNT          296 a          579 c          632 g          313 t
ORIGIN      
        1 gttgaccgcc gtgctgggga aggtgcgcga cggggtgaag ttctgtgcgg tgtgcggcaa
       61 cgtctccgac gacgaccggt gccggatctg ttcggattcg cgccgcgatg cgtcgctggt
      121 gtgtgtcgtc gaggagccga aagacgttca ggccgtcgaa cgaacccgcg aattccgcgg
      181 ccgctaccac gtcctgggcg gcgcgctgga cccgttgtcg ggcgtgggtc ccgagcagct
      241 gcgtattcgt gagttgctca acaggattgg cgagcgcgtc gacggcgtgg atatcgccga
      301 ggtgatcatc gcgaccgatc ccaacaccga aggcgaggcg acggcgacgt atctggtgcg
      361 catgctgcgc gacattcccg gactgaccgt cacgcgcatc gcctcgggcc tgccgatggg
      421 cggcgacctg gagttcgccg acgagctgac actgggtcgc gcgctgtccg gccgccgcgt
      481 gctgacctag gtgtactgag tcacatcgat caacgttccg cagagggttt ctcgcacttt
      541 ccctgccaaa cgttgaacgg tgtgacttgt cggtgcacgc gggcacgatg cgtgtatggc
      601 agaggtgttc atcggcagtg aagaactggt cgctggtcgg ctgacccgtc acgggctcca
      661 acgttggtac aggccggtct atcggggcgt gtacgcgccg aaggacgcga acttgtctct
      721 ccgcgaccga gcgatcgccg cctggctggc gtcgggccgt aaaggagtgg tggcaggcgt
      781 ggcggcttcg gcactgcatg gcgctccctg ggtggatccc gatgtgccca tcgaactcgt
      841 cggggtgaaa atccgcccac agcccgggct cgtcccacgg gccgaccgca tcgcagacga
      901 cgagataacg cgggcggcag gccttcccgt gacatcgaga acccgcacgg ctttcgacat
      961 ggggcgccac ctcgagcgga cggatgcggt cacccgcctc gacgcgctga tgtggaatca
     1021 ggagttctcg cgcgcggatg tccgggcact cggcgatcga tatccgcgcg ccgctggtct
     1081 tcggagactg ctcgaactgc ttccgctcat cgacggcgga gcggagtcgc cgcccgagag
     1141 cagggttcga ttgtggttgt cggatgccgg gttcccgcgg cccgaaacgc agattccagt
     1201 tctgcagggc agtacagctg cggtggcgtt cctcgacatg ggatggcgtg acttcatggt
     1261 cgccgtcgag tacgacgggg atcaccatcg caaagaccga cggcaatacg tcaaggacat
     1321 cgccaggctg cgaatgctcg aagcgctcgg ctggatcgtc atccgggtga acgccgagga
     1381 caaacccagc gacgtgatag cgcgagtcga agcggccctt gtccgccggg gctgctgtat
     1441 tgagatcgac gaattacagc gattcacccg gactttcgct gcctagcgcc ccctacacgc
     1501 ggcggggtgc ggcgaggcgt tcgtggcgta gcttgtcgac ctccggcagg tccagcggcg
     1561 cgaggtcgcc gacgacgcgg gccagcaggt gatccgcgag ctgagggttt cgcgccaaac
     1621 acgggccgtg cagataggtg gcgatgacgc tgccctgcac ggcaccgtcg acgccgtcac
     1681 cctcgcgatt gcccgcgccc gacaccaccc gtgccaacgg ctgcgcatcc ggccccaaca
     1741 ccgtgccgcc gcggtggttc tcgaagccgg tcagccgctc ggtgagtccg gcaggcaacg
     1801 gctgactcac cacctcgccg
//
LOCUS       DASYLX010000691         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1353256,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000691 DASYLX010000000
VERSION     DASYLX010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353256"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGP27_16315"
     CDS             complement(<1..618)
                     /locus_tag="VGP27_16315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7852899.1"
                     /translation="MRPRTLSVGLLAPPWFSVPPPAYGGTESVVNEIARGLVAAGHDV
                     VLYATGDSTAPVPLVSGLATGAWDRVGEGVVELPHVMHGYEALAGCDIVHDHTTLGPA
                     WALACGYDRVVTTCHGPFDGELRAIYRRYGKRLPVVAISRSQAASAPEIAVDRVIHHG
                     VDPDQYPLGRGDGGYLLFLGRMTPDKGVREAVAAARAAGRRLVIAA"
     gene            complement(983..1450)
                     /locus_tag="VGP27_16320"
     CDS             complement(983..1450)
                     /locus_tag="VGP27_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7852900.1"
                     /translation="MSLPVRRSAGQADNWRPVRGFEDLYAEMDRLVQSVIGSTGGDGT
                     WIPSADIFETDDAYDIEIELPGVRREDVDVRLNGNELVVTGELKERKREGLFRRRTRR
                     RGEFEFRVTLPGDLRADDVEASLAYGVLRVHVPKAQSGKPGNIQVTESGDGSR"
     gene            complement(1641..1982)
                     /locus_tag="VGP27_16325"
     CDS             complement(1641..1982)
                     /locus_tag="VGP27_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7852901.1"
                     /translation="MNIFTCALDIDEQHAVAYLNGELDMNTVHCLVDRLRPVATAGRD
                     IVIDLAGIDFFGTAGLMALDELDRHATAAGGSIRLSQLPAPVRRLLAVTRSANRFDIL
                     DHGSDLPAPHR"
     gene            complement(2188..>2320)
                     /locus_tag="VGP27_16330"
     CDS             complement(2188..>2320)
                     /locus_tag="VGP27_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852902.1"
                     /translation="HRSTLRYRLDRIRRITGCDLSVVDVRFNLHAATRAWRFLNPVD"
BASE COUNT          425 a          725 c          767 g          403 t
ORIGIN      
        1 ggccgcaatg accaggcggc ggccggccgc tcgcgctgcc gccactgcct ctcgaacccc
       61 cttatccggt gtcatccgcc ccagaaacag cagatagccg ccgtcaccgc ggcccagcgg
      121 gtactgatca gggtcgacgc cgtggtggat gacgcggtcg acggcaattt ccggtgcgct
      181 ggcggcctgg ctccgcgata ttgccaccac cggcagccgc ttgccgtagc ggcggtagat
      241 ggcccgcagt tcgccgtcga acggaccgtg gcatgtcgtg acgacgcggt cgtagccaca
      301 ggccaatgcc cacgccggcc cgagcgtcgt gtgatcgtgc acgatgtcgc agccagcgag
      361 cgcctcgtag ccgtgcatga catgtggaag ttccacgaca ccctcgccga cgcgatccca
      421 ggcgcctgtg gcgagcccgg agacgagggg caccggagcg gtgctgtcgc ccgtggcata
      481 gagcacgacg tcatgtcccg cggcgaccag gccgcgtgca atttcattta ccaccgactc
      541 cgtgccgccg taggccggtg gcggaaccga aaaccacgga ggcgccaata gtccaacgct
      601 tagagttcga ggtctcatcg accaatcgtc agaaacgagg ggtgcccacc gcaccggccc
      661 ggcggagcag aaaacaccga cgatggtagt cccgccgaaa ctagtctcgt gaccccctgt
      721 ggtacttcat gtttcgcttc gcagcggatg aaacgaacgg tgaggtcacc cgtcgcgatc
      781 gcgcggggac tcggccgttg ctccgtaaat agatggctgc cgcaatggga atggcaatga
      841 gcgctccgag ggtccccgtc gcgtcggagt gtcctggccc gccgggtgat gatgcggccg
      901 gcggtgcaaa gacaacgcct ctctccggcc gaatactgat cgaagaatcg acctggagag
      961 agagcgaaag tgcctggggc gctcagcgtg agccgtcgcc cgactcggtc acctggatat
     1021 tgccgggctt gccgctttgc gctttgggca catgaaccct cagcaccccg taagccagcg
     1081 aagcttccac gtcgtctgcc cgaaggtcac ccggcagcgt gacacggaac tcgaactcgc
     1141 cacgccgccg ggtacggcgg cgaaacaggc cctcgcgctt gcgctctttg agctccccgg
     1201 tcaccaccaa ttcattgccg ttgagcctga cgtcgacgtc ttcgcggcgc acgcccggca
     1261 attcgatctc gatatcgtag gcgtcatcag tctcaaagat gtcggccgag gggatccagg
     1321 tgccatcacc tccggtactg ccgatcaccg actgcacaag gcggtccatt tccgcataca
     1381 ggtcctcgaa cccacgaacg gggcgccagt tgtccgcctg gccggcggaa cgtcgcacgg
     1441 gcagagacat gtcaagtcct ctcatctgat ggttgcgccc ctagattgac ctatcgaagc
     1501 cgaccggcgc ttcgcgctga cgagatgaat cggaccccgt gaattcactc gccgggatca
     1561 acagccggca gaggaggtac gcggccgacg gaggcgccat cgaaacagat cgggtcaaag
     1621 tgactgaccg tccgagtgtt tcaccggtgc ggagctggta agtccgaccc gtggtccaga
     1681 atgtcgaatc gattcgccga tctggtcacc gcgagcagcc gccgcacggg ggcggggagt
     1741 tgagacaacc ggatcgagcc accggccgcg gtggcatggc ggtccagctc gtcaagcgcc
     1801 atcagtcctg cggtgccgaa gaaatcgatg ccggcaaggt cgatgacgat gtcgcggccg
     1861 gccgttgcta caggtcgtag tcgatcgacg aggcaatgca ctgtgttcat gtccagttcg
     1921 ccgttcaggt aggcgacagc atgctgctca tcgatgtcca gcgcgcatgt gaatatgttc
     1981 atcgtttcgc tccattcacc gggtcggttc atgctgaggg cacactctgc caatccgaac
     2041 ccgatcgctg catcgaccct ccagggcgaa agaaggtcaa ccagtcagac cttcgggtac
     2101 gggcatgcgc gcgtcggcac gtggcggtgg tgttggtttt tgtgggtggt gggtggtggg
     2161 gggtgggggt gggtggttaa tgtgtggtta gtcaaccgga ttgaggaagc gccaggcgcg
     2221 ggtcgcggcg tgcaggttga accggacatc gaccacgctg aggtcgcagc cggtgatgcg
     2281 ccggatgcgg tcgaggcggt agcgcagggt gctgcggtgg
//
LOCUS       DASYLX010000692         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2012677,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000692 DASYLX010000000
VERSION     DASYLX010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012677"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGP27_16335"
     CDS             <1..193
                     /locus_tag="VGP27_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7852903.1"
                     /translation="ALRLELQQDYEDTLVNPYIAAERGYVDAVIPPSHTRGYVATALR
                     LLERKITQTPPKKHGNIPL"
     gene            247..531
                     /locus_tag="VGP27_16340"
     CDS             247..531
                     /locus_tag="VGP27_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HEV7852904.1"
                     /translation="MSHDENHITEVSDPREMTIDAPPVQAVEQITILKGAPTDEELAA
                     LVAVLSAGTGGIVEPGPQELNLWGHPVDKLRFETTSWQRVTLLERTYMRR"
     gene            560..1198
                     /locus_tag="VGP27_16345"
     CDS             560..1198
                     /locus_tag="VGP27_16345"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEV7852905.1"
                     /translation="MTRVVLASASTGRRMVLRQAGIDPVVVVSGVDEDAVIAAQGPDA
                     APGDIANALASAKAEQVIANLDPSLAADCVVIGCDSMLFIDGALRGKPTSVEDARRQW
                     ESMSGRAGLLYTGHCVIRLRDGSVTDRATEFGRTTVNFGKPTGTELTAYLASGESLRV
                     AGAFTIDGLGGWFVDSIEGDPSNVIGLSLPTMRTLFTRAGLSIADLWAENPL"
     gene            1208..2095
                     /locus_tag="VGP27_16350"
     CDS             1208..2095
                     /locus_tag="VGP27_16350"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV7852906.1"
                     /translation="MPLPPDSDPSLAAYAHPERLVTSDWLSGNLGRPGLVVVESDEDV
                     LLYDTGHIPGAVKIDWHTDLNDRQVRDYINGEQFADLMNRKGISRDDTVVIYGDKSNW
                     WAAYALWVFTLFGHPDVRLLDGGRDLWISDGRETTLDVPTKQTSGYPVVVRDDAPIRA
                     FKDDVLPILGDQPLIDVRSPQEYTGERTHMPDYPEEGALRGGHIPTARSIPWGKAAQD
                     SGRFRSREELDELYSFLTPEDKTVVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTE
                     WGNAVRVPVAVGEVPGGAP"
     gene            2092..2505
                     /locus_tag="VGP27_16355"
     CDS             2092..2505
                     /locus_tag="VGP27_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEV7852907.1"
                     /translation="MTVMPAPLAEVVSDFKEMQGQDKLQLLLEFAGELPALPADLEEA
                     AMEPVPECQSPLFLHVDADDRDHVRLYFSAPPEAPTTRGFASILAQGLDNQPADDILA
                     VPDDFYSELGLAALISPLRLRGMSAMLARIKKHLR"
     gene            complement(2631..3086)
                     /locus_tag="VGP27_16360"
     CDS             complement(2631..3086)
                     /locus_tag="VGP27_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852908.1"
                     /translation="MPRFNADATRSGAEWLVHVPELEQSIIAQRRDEVIAKAYDLIQT
                     KSGLSEDQIELVLRSERDIVRSRAVRLGFEIVGESGSRTDTFQLPGKPRAVSVRYDDY
                     GAVVAVDASTQVAEFQAAREGAFDTLFYTAQYSDIRRRHLRTQVPPEAP"
BASE COUNT          630 a         1196 c         1115 g          598 t
ORIGIN      
        1 cgcgctgcgc ctggaactgc agcaagacta cgaggacacc ctggtgaacc cgtacatcgc
       61 cgccgagcga ggctacgtcg acgccgtcat cccgccgtcg cacacccgcg gctacgtcgc
      121 caccgcgctg cggctgctcg agcgcaagat cacccagaca ccgccgaaga aacacgggaa
      181 cattccgctg tgagcggccg agtgcagttc gcgcagcgaa cgtagcgaga gagcgaaccg
      241 acagccatga gccacgacga aaaccacatc accgaggtct ccgacccgcg cgagatgacc
      301 atcgacgcac cgccggtgca ggcggtcgag cagatcacga tcctcaaggg cgcgcccacc
      361 gacgaggagc tcgccgcgct ggtcgcggtg ctcagcgccg gaaccggcgg catcgtcgaa
      421 cccggcccgc aggagctcaa tctgtggggt cacccggtcg acaagctgcg cttcgagacc
      481 accagctggc agcgggtgac actgctggag cggacgtaca tgcggcgctg atgagccgct
      541 tgcgcgaaga atgtcgatca tgacgcgcgt ggtgctcgca tccgcctcta cggggaggcg
      601 aatggtgttg cgccaggcgg gaatcgaccc cgtcgtcgtc gtctccggcg tcgacgagga
      661 cgccgtcatc gcggcgcagg gccccgacgc tgcgccgggt gatatcgcca acgcactggc
      721 aagcgccaag gccgagcagg tcatcgcgaa cctcgatccg tcgctggccg cggattgcgt
      781 tgtcatcggc tgtgattcga tgctcttcat cgacggcgcg ctgcggggga aaccgacgtc
      841 ggtcgaggac gcccgtcgtc agtgggagtc gatgagcggc cgggcgggcc tgctgtacac
      901 cggacactgc gtgatccggc tgcgcgacgg ttccgtgacg gaccgcgcaa ccgagttcgg
      961 ccggaccaca gtcaatttcg gcaaaccaac cggcacggag ctgacggcgt atctcgccag
     1021 cggcgagtcg ctgcgggtgg cgggcgcctt cacgatcgac gggctcggcg gctggttcgt
     1081 cgacagcatc gagggcgatc cttcgaacgt gatcggtctt tcgctgccca cgatgcgcac
     1141 gttgttcacc cgcgcggggc tttcgatcgc cgatctttgg gctgagaacc cgctgtaagc
     1201 tcgtggagtg ccgcttcctc cagattccga tcccagcctc gccgcctacg cccacccgga
     1261 acgcctggtc acctccgact ggctatcggg caacctcggc aggccggggc ttgtcgtcgt
     1321 cgagtcagac gaggacgtgc tgctctacga caccggccac attcccggcg cggtgaagat
     1381 cgactggcac accgacctca acgaccggca ggtgcgcgac tacatcaacg gtgagcagtt
     1441 cgccgacctg atgaaccgca agggcatctc gcgcgacgac accgtggtca tctacggcga
     1501 caagagcaac tggtgggccg cctacgcgct gtgggtgttc actctcttcg gccacccgga
     1561 cgtgcgactg ctcgacggcg gccgcgacct gtggatctcc gacggccgcg aaaccaccct
     1621 cgacgtgccg accaagcaga ccagcggcta ccccgttgtc gtccgcgacg acgcgcccat
     1681 cagggcgttc aaggacgacg tgcttcccat ccttggcgac cagccgctga tcgacgtccg
     1741 ctccccccag gagtacaccg gcgaacgcac ccacatgccg gactaccccg aggagggcgc
     1801 gctgcgcggc ggccatatcc cgaccgcccg ctcgatcccc tggggtaagg ccgcacagga
     1861 cagcggccgg ttccgcagcc gcgaagaact cgacgagctg tacagcttcc tgacaccgga
     1921 agacaagacc gtggtctact gccgcatcgg cgagcggtcc agccacacct ggttcgtgct
     1981 gacccatctg ctcggcctgc ccggcgtgcg caactacgac ggctcgtgga ccgaatgggg
     2041 caacgccgtg cgggtgcccg tggcagtcgg agaggtcccc ggcggagcgc catgaccgtc
     2101 atgccggcgc cgctggccga agtcgtgtcg gacttcaaag agatgcaggg ccaggacaag
     2161 ctgcagctgc tgctggaatt cgccggtgag ctacctgcac tgcccgccga cctcgaggag
     2221 gcggcgatgg agccggtgcc ggaatgccag tcgccgctct tcctgcacgt cgacgccgac
     2281 gaccgtgacc acgtgcggct gtacttcagc gctccgcccg aggcgccgac gacgcggggt
     2341 ttcgcgtcca tcctggccca ggggctcgac aatcagcccg ccgacgacat cctcgccgtc
     2401 cccgacgact tttacagcga actgggcctg gccgcgttga tcagcccgct gcggctgcgt
     2461 ggcatgtcgg cgatgctcgc ccgcatcaaa aagcacctgc gatagcccag tgcatctgcg
     2521 ctcactttga gttggccgca cctttggccc gaagcatcgt cgagaagccg gttgcagcat
     2581 cgtcggcgag tcggtatcag gtgccgccag tgccattgaa gagcgcgtcg ctacggggct
     2641 tcaggaggaa cttgtgtccg taggtgacga cgtctgatgt cgctgtattg agccgtatag
     2701 aacaatgtgt cgaacgcccc ttcgcgcgcg gcttgaaact ccgcgacctg tgttgaagcg
     2761 tcgacggcga cgaccgcgcc gtagtcgtcg tatctgacgg acaccgctct gggttttccg
     2821 gggagctgaa acgtgtcggt acgcgatccg ctttcgccaa cgatctcgaa tcccagccgc
     2881 actgctcgcg atctcacaat gtcacgctcc gaacgcaaca ccagctcaat ctggtcctcc
     2941 gacagtccgg actttgtctg tatcaggtca tacgccttag caatgacctc gtcgcgacgt
     3001 tgggcgatga tggactgctc gagttcgggg acgtgaacca gccactccgc accgctgcgc
     3061 gtcgcgtcgg cgttaaaccg tggcaacttc cgtcccctag ggccaagtgt tgagtttgcg
     3121 tctctgcctt cagcggcgcg tcaattgcgc gtcacatcgt tcgcaaggga aagccgtctg
     3181 gtcggccgtc acccactgta tgtgtcggca gatagtgcac cgtacgttcg tgccctgaat
     3241 tgcgggctcg ctctgactca tggcggcaaa ggtacgaccc ctcacgcaag atcactgcag
     3301 attggccgct tgaaggaact ctttcttacg cgcgccgacg tcttcactcc atcactgacg
     3361 cggtcgccga ccgccgttcg cccatggccg gtgaagctag ccgtacggct accgagggtc
     3421 aattggggtc ggcacggcgg cggcgcttaa actgcccggg atacattctt aaagacgatt
     3481 cttagatacc tgtcgatcag gaggcgcagt gcccagtcac gccagctcac gcatccata
//
LOCUS       DASYLX010000693         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2560038,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000693 DASYLX010000000
VERSION     DASYLX010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560038"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGP27_16365"
     CDS             complement(<1..204)
                     /locus_tag="VGP27_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852909.1"
                     /translation="MYAEQHDEFWERLHDAERLSAAALMSVGPDGASRRARALEIFDR
                     VPELETWTYGPEAHCDDAVRAELL"
     gene            complement(666..2093)
                     /locus_tag="VGP27_16370"
     CDS             complement(666..2093)
                     /locus_tag="VGP27_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7852910.1"
                     /translation="MFTDVEGSTRRWEADADAMRAALQASAQRYAFAIAKGQEPPMPG
                     HRQHAVDLRVQQVAPDSQRTLLLGTLLQFALRAVLTVFVVVTLLCEPPNRYRWICVAV
                     LTVYVLVIGCWSAWALGTAARAVLPARPPVTLLVLAADVALVSVLSVLTGITSPDDWT
                     SDVMRNGFFLIPLIAAAQLNPIISGAVAVPTLSAFIATCWITRSSNQEPWASILLSST
                     VLAALAGGSVALSLIQRSKVEMIADLARQRTQLLEELLGLEKRERQAISERLHDGALQ
                     YVLVARLDMEDVRDGLKAAADRVESALVECSGLLRDVVRELHPDVLARLGLKSAISGL
                     TDSLCSRTELSVDLAAPTWPDGLHTEADYVLYSAAREALTNVIKHAGAHHIWIELEHH
                     EELASLRISDDGVGISEATIARKAEEGHIGMTSIRVKVLASGGQFDVRSTSPGTEVTM
                     SIPLRPAFFIPNAGELVNARVEHKR"
     gene            complement(2430..2542)
                     /locus_tag="VGP27_16375"
                     /pseudo
     CDS             complement(2430..2542)
                     /locus_tag="VGP27_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
BASE COUNT          536 a          838 c          796 g          485 t
ORIGIN      
        1 gagtagttcc gcgcggacgg cgtcgtcgca gtgggcctcg ggaccgtacg tccaggtttc
       61 gagttcggga acgcgatcga agatctcaag ggctcgggcg cggcgcgagg caccgtctgg
      121 gccgaccgac atcagcgccg cggcggagag gcgctcggca tcgtgcagcc gctcccagaa
      181 ctcgtcgtgc tgttccgcgt acacatctgg cctcctcacg tgcctggtgg agagccgatc
      241 atcgcgacat gatctacctg ccatttgaac tggacgaatg gtgaatgtca taacgggaca
      301 ggcgctgcag ctgccgggtt tgccgtactt caacacgaca gtgtcatcag tcgtcagcgc
      361 aggggtctag cgagacgtac gaatagcgat cgggtgtagc tggccccaca tagctgccat
      421 catgaaacaa cagcacgtgc cttggcgggg tttgctgttc ttcaccgaaa tccgtcgcgg
      481 gatcgatggc tatttcgtat gaatgatcca ccccgggcga gttctgccgc cggatagagc
      541 aggcggtagc tgcatacggg gtggccagac gtgccgtgca gctcccggca agcggtgacc
      601 ttagcgatat ggcgttcgcc cagcagcctg ctgggacggt gctgggatga gattggcctc
      661 gacagctatc tcttgtgctc gactctagcg ttcaccaact cgccagcatt cgggatgaag
      721 aatgccggcc gcagcggtat ggacatggtg acttcagttc cgggagatgt cgagcgcacg
      781 tcgaactgtc ccccggacgc gagtaccttg acgcgaatcg atgtcattcc gatatgacct
      841 tcttcagctt tccgcgcgat agtcgcctcc gagatgccca ccccgtcgtc ggaaatacgc
      901 aatgaggcca gctcttcgtg gtgttccaac tcaatccaaa tgtggtgggc ccctgcatgc
      961 ttgatgacgt tcgtcaacgc ctcacgcgcc gcgctgtaca gcacgtaatc cgcctcggtg
     1021 tgcaggccat caggccatgt aggtgcggcg agatcgaccg aaagctctgt acgcgaacag
     1081 agactatcgg tgaggccgga gatcgccgat ttcaatccca gccgcgcgag cacgtccgga
     1141 tgcagttcgc ggaccacatc gcgcagtagg cccgagcact cgacgagcgc cgattccacc
     1201 cggtccgccg cagcctttaa gccgtcccgc acgtcctcca tatctaggcg cgcgaccaag
     1261 acatactgaa gggcaccgtc gtgcagacgt tcagaaattg cctggcgctc acgcttttcc
     1321 agtcctagca actcctccaa caactgggtt cgttgccgcg ccaagtctgc gatcatttct
     1381 actttggacc gctggatgag cgataaggcg accgagccgc cggcgagtgc tgccaacacg
     1441 gtcgagctca acagaatcga cgcccacggc tcctgattgg acgatctggt gatccagcac
     1501 gtggcaatga atgcggataa ggtcggaact gcgacggccc cgctgatgat gggattcaac
     1561 tgcgccgccg cgatcaaagg gatcaggaag aaaccattcc tcatcacgtc cgatgtccag
     1621 tcatccggcg aggtgatgcc ggtcagaacc gacaggacgg ataccagcgc gacgtcggcg
     1681 gccagcacta gcagtgtcac cggcggtctg gcgggtaaca ctgcgcgcgc cgcagtcccc
     1741 agcgcccacg cgctccagca tccgatcacg agcacgtaga ccgtcaagac agccacgcag
     1801 atccatcggt agcggttcgg cggctcacac aacagagtca cgacgacgaa aacggtcagt
     1861 accgcgcgca acgcaaactg cagcagagtg cccaacaaca aagtccgctg ggagtccggc
     1921 gcaacttgtt gcactcggag gtcgaccgca tgctgacgat gtcccggcat gggcggctcc
     1981 tgtccctttg cgatggcgaa agcataacgc tgggcgctcg cttgcagggc cgcccgcatc
     2041 gcgtccgcgt cagcctccca ccgacgggtc gaaccctcga catcggtgaa caagaacgtc
     2101 accacccccg aaggagcagc cgccggatca gcggaaacgc acgcagaatc tcacaacaga
     2161 cacgccccta cctcgtacat cgtcggaatc cggcccggtc accagataca cgtgttttga
     2221 gacgaacttc cgcgagtcgc agatgtcgtc tatttcgaga ccgtgttcag ctctgctcgc
     2281 gcctgctcga tttggtcgta ggcatacgtc accatctcgg cggtggtcat cacctcgccc
     2341 ttgtgagcga gcgattcgta ggccgggtcg ccgaggacat cgcgcaggtg ggcgatggcg
     2401 gtattgatct ccgggaaggc gtcggcgtgg ttgatgttta tccagaaccc ccggattcga
     2461 tcagaccgcc cttgcggccg tcgatgcgcg cccgcgtctc acaccaccgg gtagaggtgg
     2521 ctgaacacca gcggcgcagc cgccatcgag cagacgatct ggacccgcca gcaggaagta
     2581 tcctgaattt gaaagacgtt gtccaccacc tggatcgggg atcgcagtgc acctcgatcc
     2641 gcttctccga acggc
//
LOCUS       DASYLX010000694         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1683512,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000694 DASYLX010000000
VERSION     DASYLX010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683512"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGP27_16380"
     CDS             complement(<1..602)
                     /locus_tag="VGP27_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7852911.1"
                     /translation="MSPANPLPPISRRRALTAFAVGVVAPGALAACAGKSKAPSEAAE
                     APSAPSLTYSPANAATDVTPTTPVRVEVRDGWFQRVSLTNADGKVVAGAINRERTAFN
                     VTEPLGYGVDYKWSGAVVGRDGKAVPVKGNFTTVNPSTQVNGQFQLADGQVVGVAVPV
                     ILQFDATIADEDRVTVEKALKVTANPPVEGSWAWLPDEVGG"
     gene            complement(618..1685)
                     /locus_tag="VGP27_16385"
     CDS             complement(618..1685)
                     /locus_tag="VGP27_16385"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV7852912.1"
                     /translation="MVSSLVGGVPVAEQVPLAPLTTLRVGPVAGRMITCETTDAIISV
                     VRALNGENTSGERVLVLAGGSNVVLGDEVPDLTVVRLANAGISVDGNILRAEAGAVWD
                     DVVVTALEHGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTIRRVRLLDRGTGDTRW
                     VDGEELGFGYRTSILRHSEAAIALEVEFALDAGGRSAPLRYGELTAALGAEPGQRIDP
                     AAVRDAVLALRKRKGMVLDEADHDTWSVGSFFTNPVVTAADFDAIQARSDVPVPNYPA
                     PNGVKLAAGWLVEHAGFGRGYPGDEAPARLSTKHALALTNRGGATAADVIALARRVHD
                     GVKATFAIELTPEPILIGASF"
     gene            1712..2218
                     /locus_tag="VGP27_16390"
     CDS             1712..2218
                     /locus_tag="VGP27_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEV7852913.1"
                     /translation="MPRSFDMAAEYDGSVEQVYRALRDQNYWHERLADSGADEATLDS
                     MTVDDGGIDVVTTQVLRRDRLPGVVTQFHRGDLSIVREEAWSPVRDGTATATVTGAIP
                     GAPVSLTGTAVLAPTDGGGSRLQFTASVEVRIPLVGGKVENFIGGQLVELLIAEQRFT
                     TAWIKENP"
     gene            2239..2754
                     /locus_tag="VGP27_16395"
     CDS             2239..2754
                     /locus_tag="VGP27_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEV7852914.1"
                     /translation="MARRMDYRICYDAPAEKIFGDFAGRDYWETLMDAYRFLTPQSEI
                     TTFTTNESGTDIVFKQNLPRMYLPPVARSVMPVDMIITREQHFDPYDHANRLGNGTYR
                     ASIPHGPGHFGGKYLLTETDSGSELRLVSVCKVYIPLIGGALEDLILHHIKALFDAEE
                     AFTADWIARHH"
     gene            2814..>3277
                     /locus_tag="VGP27_16400"
     CDS             2814..>3277
                     /locus_tag="VGP27_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852915.1"
                     /translation="MGAPVGQLTRGTTGHNRLRRCDRWLVHSPRVRTALQSAADPVVV
                     DLGYGALPVTTLELASRLRTVRIDVRVIGLEIDPARIVPPQGGVEFGLGGFELAGHRP
                     VLVRAFNVLRQYPEDAVDEAWSLMKTRLAPGGLIIDGTCDELGRRCCWVLIDG"
BASE COUNT          535 a         1141 c         1099 g          502 t
ORIGIN      
        1 ccgccgacct cgtcgggcag ccacgcccaa ctgccctcca ccggcggatt cgcggtcacc
       61 ttcagcgcct tctccacggt gaccctgtcc tcgtcggcga tcgtcgcgtc gaactgcagg
      121 atgaccggga ccgcgacccc gacgacctgg ccgtcggcga gttggaactg gccgttgacc
      181 tgagtcgacg ggttgaccgt ggtgaagttc cccttcacgg gcacggcctt gccgtcacgg
      241 ccaaccaccg cgcccgacca cttgtagtcg acgccgtagc cgagcggttc ggtgacgttg
      301 aaggcggtgc gctcgcggtt gatcgcgccg gcaaccacct tgccatcggc gttggtgagg
      361 ctgacccgct ggaaccagcc gtcacggacc tcgacgcgga ccggcgtcgt cggagtcacg
      421 tcggtagcgg cgttcgccgg gctgtaggtc agcgaaggcg ccgacggggc ctcggcggcc
      481 tcggacggcg ccttggactt accggcgcac gcggccaggg cgcccggagc aaccaccccg
      541 accgcaaatg cggtcagggc ccggcgccga gatatcggcg gcaacggatt ggctgggctc
      601 acgtgttcca aacataccta gaaagaggct ccaatcagaa tcggctcggg tgtgagctca
      661 atcgcgaacg tcgccttcac gccgtcgtgt acccgcctgg ccagggcaat cacgtccgca
      721 gcggtggctc cgccgcggtt ggtcagcgcc agcgcgtgct tggtggacag tcgcgccggg
      781 gcctcgtcgc cggggtagcc cctgccgaag ccggcatgtt cgaccagcca gccggcggcc
      841 agcttgacgc cgttcggcgc gggatagttg ggcaccggga catccgagcg cgcctggatc
      901 gcgtcgaagt ccgcggctgt gacgaccggg ttggtgaaga acgacccgac gctccaggtg
      961 tcatggtcgg cctcgtcgag caccatgccc ttgcgtttgc gcaatgccag cacggcgtcg
     1021 cgcaccgcgg ccggatcgat gcgttgaccc ggttcggcgc cgagcgcagc ggtcagctcg
     1081 ccgtaccgca acggcgcgct gcggcccccg gcgtcgagcg cgaactccac ctccaacgcg
     1141 atggcggcct cggagtgtct gagaatgctt gtccggtaac cgaatccgag ctcctcgccg
     1201 tcgacccacc gggtgtctcc agtgccgcgg tccagcaacc ggacgcgtcg gatcgtgtcg
     1261 gagacctccg ccccgtacgc gccgacgttc tgtaccggcg tcgcacccgc cgagcccgga
     1321 ataccggaca ggcattcgag accgccgagg ccgtgctcca gtgcggtgac gacaacgtcg
     1381 tcccacaccg cacccgcctc cgcgcgcaag atgttgccgt cgacgctgat gcccgcattg
     1441 gccagccgga ccaccgtcag gtcgggcacc tcgtcgccga gcacgacgtt ggagccgccc
     1501 gccaacacca gcacccgttc accggaggtg ttctcgccgt tcaaggcgcg gacgacggag
     1561 atgatcgcgt cggtggtctc gcaggtgatc atgcggcccg cgaccgggcc gacgcgcaac
     1621 gtggtcagcg gcgcgagcgg cacctgctcg gcgaccggta caccacctac cagcgaactg
     1681 accacgggcc gtaacggtag cctggccagc tatgccgcgt tcattcgaca tggccgccga
     1741 atacgacggc agcgtcgaac aggtctaccg ggcattgcgc gatcagaact actggcacga
     1801 gcggctggcc gattccggcg cagacgaggc gacgctggat tcgatgaccg tggatgacgg
     1861 cggcatcgac gtggtgacga cgcaggtgct gcgccgcgac cgactgcccg gcgtggtgac
     1921 gcagttccac cggggcgatc tgtccatcgt gcgcgaggag gcctggagcc cggtgcgcga
     1981 cggcacggcc acagccaccg tgaccggtgc cattcccggc gcgcccgtat cgctgaccgg
     2041 caccgcggtg ctcgctccca ccgacggcgg cggctcgcgc ctgcagttca ccgccagcgt
     2101 cgaggtgcgg atcccgctcg tcggcggcaa agtcgaaaac ttcatcggcg gccaactcgt
     2161 cgagttgctc atcgccgagc agcggttcac cacggcgtgg atcaaagaga acccgtaacc
     2221 ggccggtaac ctggtgttat ggcgcgccgg atggactaca ggatctgtta cgacgcgcct
     2281 gcggaaaaga tcttcggaga cttcgccggc cgcgactact gggaaaccct gatggacgcc
     2341 taccggttcc tcactccgca gtccgagatc accacgttca cgaccaacga gtcgggcacc
     2401 gacatcgtct tcaaacagaa cctgccgcgc atgtacctgc cgccggtcgc acgcagtgtg
     2461 atgcccgtcg acatgatcat cacccgcgag cagcatttcg acccgtacga ccacgccaac
     2521 aggctgggca acggcaccta ccgggcgagc atcccgcacg ggccgggaca cttcggcggc
     2581 aaatacctcc tcaccgagac cgattcgggc agcgagcttc ggctcgtcag cgtctgcaag
     2641 gtctacatcc cgctcatcgg cggagcgctg gaggatctga tcctgcacca catcaaggcg
     2701 ctcttcgatg ccgaagaggc gttcaccgcc gactggatcg ccagacacca ctagcgcctc
     2761 aggcgtacgg cgtggatcag ggcggacggg gtcggacgcc gtaaaatcgg atcatgggcg
     2821 ctcccgtcgg acagctgacg cggggcacca ccggtcacaa ccggctgcgg cgctgcgatc
     2881 gctggctggt gcactcgccg cgggtccgca ccgcgctgca atcggccgcc gacccggtgg
     2941 tcgtcgatct cggctacggc gcgctgccgg tgaccaccct cgagttggcc agccggttgc
     3001 ggacggtgcg catcgacgtg cgggtgatcg gcctggagat cgatcctgca cggatcgttc
     3061 cgccgcaagg aggcgtcgaa ttcggtctcg gcggcttcga gttggccggc caccggccgg
     3121 tgctggtgcg cgcgttcaac gtgctgaggc agtacccgga ggacgccgtc gacgaggcct
     3181 ggtcactgat gaagacgcgg ctcgcgccgg gcggcctgat catcgacggc acctgcgacg
     3241 agctgggcag gcggtgctgc tgggtgctga tcgacgg
//
LOCUS       DASYLX010000695         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3218394,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000695 DASYLX010000000
VERSION     DASYLX010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218394"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGP27_16405"
     CDS             complement(<1..239)
                     /locus_tag="VGP27_16405"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969879.1"
                     /note="catalyzes the formation of undecaprenyl
                     pyrophosphate from isopentenyl pyrophosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV7852916.1"
                     /translation="MKRAAKTPYPQLPPAPDDYPTFPDKSSWPVVFPALPPHSNGRFA
                     RPPQHTSKAAAPKIPADQVPHHVAVVMDGNGRWAT"
     gene            complement(190..1035)
                     /gene="recO"
                     /locus_tag="VGP27_16410"
     CDS             complement(190..1035)
                     /gene="recO"
                     /locus_tag="VGP27_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV7852917.1"
                     /translation="MRLYRDRAVVLRQHKLGEADRIVTLLTRDHGLVRAVAKGVRRTR
                     SKFGSRLEPFAHIDVQLHPGRNLDIVTQVQAIDAFASDIVSDYGRYTCACAVLETAER
                     LAGEEHAPVPALHRLTVGALRAVADGSRPWELVLDAYLLRAMGIAGWAPALTACARCA
                     APGPHRAFHIAAGGSVCVHCRPSGSTTPPQAVLDLMSALYDGDWDVAQASQQSHRNQA
                     SGLVAAHLQWHLERQLRTLPLVERVYRVDRAVAEHRVSLFGQDVTRGDETGGQDPVPS
                     AASGA"
     gene            1100..2536
                     /locus_tag="VGP27_16415"
     CDS             1100..2536
                     /locus_tag="VGP27_16415"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7852918.1"
                     /translation="MAQPSMPTLTGLLYRLASGRVTSVELTKQSLDAIEASQPTLNAF
                     RVVLRKQALADAARADRRRAAGQQAPLLGVPIAVKDDVDVAGVPTMFGAAGRVRSSVA
                     DAEVVRRLRTAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRNHTPGGSSGGSAAA
                     VAAGLVTAAIGSDGAGSVRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLART
                     VIDAALLLDAASGNVDGDRHKPAPIQAAEAVGRAPGPLKVALSTKFPFNVFRPKLNPD
                     IRAALHTVGEQLTELGHTIVAADPDYKIGMSWNFLSRSTSGLMDWADRLGHDVELDGR
                     TVENLRMGRLLSENVLRKARAKEAAAQRRVGYIFNLVDVILAPTTALPPPTVHHFDKR
                     GGFSTDRAMIRACPATWPWNLLGWPSINVPAGFTSEGLPIGVQLMGPADSEPLLISLA
                     AELEAVNGWVTRQPEPWWTESAAGVAGG"
     gene            complement(2561..>2717)
                     /locus_tag="VGP27_16420"
     CDS             complement(2561..>2717)
                     /locus_tag="VGP27_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV7852919.1"
                     /translation="IGKGGARLREVGTAARTQIEKLLGTKVYLDLRVKIAKNWQRDPK
                     QLGRLGF"
BASE COUNT          427 a          903 c          961 g          426 t
ORIGIN      
        1 tgggtggccc agcggccgtt gccgtccatg acaaccgcga cgtggtgggg cacctggtcg
       61 gccgggatct tgggtgcggc ggccttcgac gtgtgctgag gcggacgcgc gaacctgccg
      121 ttgctgtgcg gcggcagcgc cggaaagacg accggccacg acgacttgtc agggaaggtc
      181 gggtagtcgt caggcgccgg aggcagctga gggtacgggg tcttggccgc ccgtttcatc
      241 gccacgtgtg acatcctgcc cgaacagcga gacgcgatgc tcggcgaccg cgcgatcaac
      301 ccggtagaca cgctcgacca gcggcaatgt ccggagttga cgctcgagat gccactgcag
      361 gtgcgcagcc accagcccgc tggcctggtt gcgatgcgac tgttgcgagg cctgcgcgac
      421 gtcccagtcg ccgtcgtaga gcgccgacat cagatcgagc acggcctgcg gcggtgtggt
      481 tgatccggac ggcctgcaat gcacgcacac gctgccgcct gcggcgatgt ggaacgccct
      541 gtgcggaccg ggtgcagcgc aacgggcaca cgccgtgagt gcgggcgccc atccggcgat
      601 tcccatggcg cgcagcaggt aagcgtccag gacgagttcc cacggacggc tgccgtcggc
      661 caccgcacgc aacgcgccga ccgtcagccg gtgcagtgcg ggcaccggag cgtgctcctc
      721 gcccgccagt cgttcagcgg tttccagcac ggcgcacgca caggtgtagc ggccgtagtc
      781 gctgacaatg tcggaggcga acgcatcgat tgcctgaacc tgggtgacga tgtcgagatt
      841 gcggccgggg tgcagttgga cgtcgatgtg cgcgaacggc tccagccggg aaccgaactt
      901 gctgcgggtg cgccggacgc ccttggccac ggcacggacc aacccgtggt cgcgggtgag
      961 cagggtgacg atccggtcgg cctcgcctag cttgtgctgg cgaagcacca ccgcccgatc
     1021 ccggtatagg cgcatcgatc cagtttctca tcgtgttccg acaaggccgg taagcagcgc
     1081 cgggatattc tcgatatcga tggctcagcc ttccatgccc acacttacgg gcttgctgta
     1141 tcggctggcc agcggcaggg tgacgtcggt cgagctgacc aagcagtcgc tggacgccat
     1201 agaagccagc cagccgacgc tcaatgcgtt ccgcgtggtg ctgcgcaaac aggcgctcgc
     1261 cgacgccgcg agggccgacc gcagacgtgc cgccggacag caggctccgt tgctcggggt
     1321 gccgatcgcg gtcaaggacg atgtcgacgt cgcaggcgtg ccgacgatgt tcggcgcggc
     1381 gggccgggtc cggtcgtcgg tggccgacgc tgaggtcgtg cgccgcctgc gcaccgccgg
     1441 tgcggtcatc gtcggtaaga cgaatacgtg cgagctcggc caatggccgt tcaccagcgg
     1501 acccggcttc ggacacacac ggaacccgtg gtcgcgcaac cacactccgg gcggttcgtc
     1561 cggtggcagc gcagccgcag tggcagcggg actggtgacc gccgcgatcg ggtcggacgg
     1621 cgcgggcagc gtccggattc ccgcggcatg gacgcacctg gtcggcatca agccgcagcg
     1681 gggccggatc tcgacatggc cactgccgga ggcgttcaac ggcataacgg tcaacggcgt
     1741 gctggcccgc accgtcatcg acgcggcact gctgctcgac gccgcgtcgg gcaatgtcga
     1801 cggggaccgt cacaagccgg cgcccattca ggcggccgag gccgtcggcc gtgcgccggg
     1861 cccgctgaag gtcgcactgt cgacgaagtt cccgttcaac gtcttccgcc ccaagctgaa
     1921 ccccgacatc cgggccgcat tgcacaccgt cggcgaacaa ctcaccgaac tcggtcacac
     1981 catcgtcgcg gccgaccccg actacaagat cgggatgtcg tggaacttcc tgtcccggtc
     2041 gacgtccggg ctgatggact gggcagaccg cctgggccac gatgtcgagc tggacgggcg
     2101 caccgtcgag aatctgcgga tgggacggct gctgtcggag aacgtgctcc gcaaggctcg
     2161 cgccaaggag gccgcggcac agcgccgcgt cggttacatc ttcaatctgg tcgacgtgat
     2221 cctggcgccg acgactgcgc tgccgccgcc gaccgtgcac cacttcgaca aacgcggcgg
     2281 gttctccacc gatcgggcga tgattcgcgc ctgccccgcg acatggccgt ggaacctact
     2341 gggctggccg tcgatcaatg tgcccgccgg cttcacctcg gaggggttgc cgatcggcgt
     2401 gcagttgatg ggtccggccg acagtgagcc gctgctgatc tcgcttgccg ccgagctgga
     2461 ggcggtgaac ggctgggtga ccaggcagcc cgagccgtgg tggacggaat ccgcggccgg
     2521 tgtcgccggc ggttgattcg gggaagcggg cccggaatcg ctagaagccg agccggccga
     2581 gctgtttggg atcgcgctgc cagttcttgg cgatcttgac gcgcaggtcc agatacacct
     2641 tggtgccgag cagcttctcg atctgcgtgc gtgcggcggt gcccacctcg cgcagcctgg
     2701 caccgccctt gccgatc
//
LOCUS       DASYLX010000696         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3769480,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000696 DASYLX010000000
VERSION     DASYLX010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3769480"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGP27_16425"
     CDS             complement(<1..196)
                     /locus_tag="VGP27_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852920.1"
                     /translation="MTADERSREEQTSSKIQQIPDISATPEWQALARHHDQIRDVHLR
                     ELFDEDPDRGTELTLTVGDLY"
     gene            245..1693
                     /locus_tag="VGP27_16430"
     CDS             245..1693
                     /locus_tag="VGP27_16430"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7852921.1"
                     /translation="MDTTALLKSVPTGIWIGGEEREGSSTFDVLDPSDDSVLISIANA
                     TSEDAIAALDAAAAMQAEWAATAPRKRGEVLRSVFEKITERAEDLATLMTLEMGKVLR
                     ESMGEVTYGAEFFRWFAEEAVRIHGRYTESPAGTGRIVVTKQPVGPCYAITPWNFPLA
                     MGTRKIGPAIAAGCTMIVKPAQETPLTMLLLAKLMDEAGLPKGVLSVLPTTDPRAITT
                     ALIDDGRLRKLTFTGSTGVGKALVKQSSDALLRTSMELGGNAPFIVFDDADIDAAVEG
                     AILAKMRNGGEACTAANRFHVANSVREEFTEKLVKRMSEFQLGKGIDGSSTLGPLINA
                     KQVATVADLVSDAVSRGATVAVGGVAPGGPGHFYPATVLADVPADARILKEEVFGPVA
                     PITGFDTEEEGVAAANNTEYGLAAYIYTRDLDRALRAAEGIESGMVGVNRGVISDPAA
                     PFGGVKQSGFGREGGFEGIDEYLETKYIALTK"
BASE COUNT          321 a          616 c          652 g          290 t
ORIGIN      
        1 tgtagaggtc gccgaccgtc aacgtcagct cggtgccccg gtccgggtcc tcgtcgaaca
       61 gctcgcgcaa atgtacgtcg cgaatttgat catgatgtcg cgccaaggcc tgccattccg
      121 gagtggccga gatatccggg atctgctgga ttttgctgct tgtctgctcc tcgcgcgagc
      181 gctcgtcggc ggtcatactg cgacactagt gcggggaatt gaccaaaggt gtaagagagg
      241 aagtatggat accaccgcac tgctgaagtc agttcccacc ggcatctgga tcggcggtga
      301 ggagcgcgag ggatcgtcga ccttcgacgt gctcgacccc tccgacgact cggtcctgat
      361 ttcgatcgcc aatgccacca gcgaggacgc gatcgcggcg ctcgacgcgg cggcggcgat
      421 gcaggcggag tgggcggcca ccgcgccgcg caaacgcggg gaggtgctgc ggtcggtgtt
      481 cgagaagatc accgagcgtg ccgaggacct cgccacgctg atgacactgg agatgggcaa
      541 ggtcctgcgc gagagcatgg gcgaggtcac ctacggcgcc gagttcttcc gctggttcgc
      601 cgaggaggct gtgcgcatcc acggccgcta caccgagagc ccggcgggaa ccggccgcat
      661 cgtcgtcacc aagcagcccg tcggcccctg ctacgcgatc acgccgtgga attttccgct
      721 ggcaatgggc acccgcaaaa tcgggcccgc gatcgctgcc ggctgcacga tgatcgtcaa
      781 gcccgcgcag gagacgccgc tgacgatgct gctgctggcc aagctgatgg acgaggcggg
      841 cctgcccaag ggtgtgctgt ccgtgctgcc gacgacggat ccgcgcgcca tcaccaccgc
      901 gctcatcgac gacgggcggc tgcgcaagct gaccttcaca ggctcgacag gagtcggcaa
      961 ggcgctcgtc aagcagtcat cggatgcgct gctgcgcacg tcgatggagt tgggcggcaa
     1021 cgccccgttc atcgtgttcg acgacgccga catcgacgcg gctgtcgagg gcgccatcct
     1081 ggccaagatg cgcaacggcg gcgaggcctg cacggccgcc aaccgcttcc acgtcgccaa
     1141 ctccgtgcgc gaggagttca ccgagaagct ggtcaagcgg atgagcgagt tccagttggg
     1201 caagggcatc gacgggtcgt cgacgctggg gccactgatc aacgccaagc aggtcgccac
     1261 cgtcgcggat ctggtgtccg acgcggtgtc gcgcggcgcc acagtggccg tcggcggcgt
     1321 cgcgcccggc gggcccggcc atttctatcc cgcgacggtg ctggccgacg tgcccgccga
     1381 tgcccgcatc ctcaaggagg aggtgttcgg gcccgtcgcg ccgatcacgg gcttcgacac
     1441 cgaggaggag ggggtggccg ccgccaataa caccgaatac gggctggccg cctacatcta
     1501 cacccgcgat ctggaccgtg cgctgcgggc ggcggagggc atcgagtcgg gcatggtggg
     1561 cgtcaaccgc ggcgtgatct ccgatccggc cgcgccgttc ggcggcgtca agcagtccgg
     1621 cttcggccgc gaaggcggat tcgagggcat cgacgagtac ctcgagacga agtacatcgc
     1681 gctgaccaag tagctgggcc ggaaaagctc gcgagctggg cccacgcgaa gctagggaac
     1741 gcaaaatcgc acagacacgc cgtcgctaat gcgatcttgc gtctgctcgc gcgggaaaga
     1801 tcccatcggt gagtggccac cgatgggtcg cgccgccccc gcgcgccgtc gaccggggtg
     1861 cccccagggc gtctccccg
//
LOCUS       DASYLX010000697         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_806339,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000697 DASYLX010000000
VERSION     DASYLX010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806339"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VGP27_16435"
     CDS             <1..166
                     /locus_tag="VGP27_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7852922.1"
                     /translation="YHKKTDTLDHVDRKALQINGSGVGYVVGLYAQDQRGRNGVPVRD
                     DRTRHVLAKS"
     gene            190..1620
                     /locus_tag="VGP27_16440"
     CDS             190..1620
                     /locus_tag="VGP27_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV7852923.1"
                     /translation="MAGAVVVAMLLVGCSSAPQPSRNLARELADKIGVDGMYVHLRKL
                     QEIADANNRSRADGTPGFDATVDYVAQTLRDKGFDVQTPEYERLGVASPGQPTLTASG
                     RDYPVDQASLLTPTPKGGLRAVTQRPQKPAGCTAADYGTARMANAIAVVDDMGCSIVD
                     KQNTAVAEGALGLLVVSDAGRRGLFPPGYYRALTVPVAVIDAGADAALRRTSATVLLT
                     LDAKAAMVKSRNVLAQTKSGDPRNVVMAGAHLDTVAGSPGINDDGTGVAALLETAAAL
                     GPRPQIANAVRFGFWGAEEAVLAGSTKYVQSLNGDQLSDIALYLDFDMLGSPNAGFFT
                     YDGDQSGQANPDVPANTVPVGSAGIERTMAGYLNLAGIRPADLPLGKASDYSPFLDAG
                     VPIGGTTTGAAQRKTELQARLWGGVAGVAFDRNYHLPGDSVDNVNRDALAVTGPAVAF
                     AVGTYAQSIEGVNGVPTREQRRRHTP"
     gene            complement(1586..>2585)
                     /locus_tag="VGP27_16445"
     CDS             complement(1586..>2585)
                     /locus_tag="VGP27_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7852924.1"
                     /translation="GHASDLATVGAQKAVGTGANAVLLVRGEDTVRAFANTCRHRGHE
                     LLACNQTTRGRSIVCPYHSWSYRLDGSVRNAPGFKDVDGFDAGEFGLAELRLVNWHGW
                     LFVDPSGEDAEFSEHVAGWEDVVAPYRPEDLTVVDRHSYELATNWKVIAENYQECYHC
                     STIHPELSRISPPTSGENLDTEGSWMGGWMSIIEGAETMSLSGASGGVAIKGLSEHEL
                     RSVMYVVGYPNLLVSLHPDYVMTHLMTPLDVDRTHVECAWAFPPEVAEKPGFDPSYAI
                     DFWDLTNRQDWAACESVQRGLTSPHARPGPLAPDEDGVYQFVSRVAKAYAGGAARG"
BASE COUNT          421 a          879 c          895 g          390 t
ORIGIN      
        1 ctatcacaag aagaccgaca ccctcgacca cgtcgatcgt aaggcgttgc agatcaatgg
       61 ttccggcgtc ggctacgtcg tcggtctcta cgcgcaggat cagcgcggcc gaaacggggt
      121 gccggttcgc gacgaccgca cacggcacgt cctcgcgaag tcatgacggc aaggcggcga
      181 catcaggcca tggcgggcgc ggtcgtcgtc gcgatgctgc tcgtcggatg ctcgtcggcc
      241 ccgcagccgt cgcggaacct cgcccgcgag ctggccgaca aaatcggcgt cgacgggatg
      301 tacgtgcatc tgcgcaagct ccaggagatc gccgacgcca acaaccggtc ccgtgcggac
      361 ggcacccccg gtttcgacgc cacggtcgac tacgtcgctc aaacgctgcg ggacaagggt
      421 tttgacgttc agacgcccga gtacgaacgc ctcggcgtgg cgtcgccggg acagcctacg
      481 ctgaccgcat cgggccgcga ctaccccgtc gaccaggcgt cgctgctgac tccgaccccg
      541 aagggcggat tgagggccgt cacgcagcgt ccccagaagc ccgccggctg caccgccgcg
      601 gactacggaa cggcgaggat ggccaacgcc atcgcagtcg tcgacgacat gggatgctcg
      661 atcgtcgaca agcagaacac cgccgtcgcc gagggcgcac ttggacttct cgtcgtgagc
      721 gacgcgggaa ggcgggggtt gttcccgccg ggctactacc gggcgctgac ggtgccggtc
      781 gcggtgatcg atgcaggcgc cgatgcggcg ctgcggcgaa ccagtgcgac ggtgctgctg
      841 acgctcgacg ccaaggcggc catggtgaag tcacgaaacg tgctggcgca gaccaagagc
      901 ggtgaccccc gcaatgtggt gatggcgggc gcgcatctgg acaccgtcgc cggaagccct
      961 ggcatcaatg acgacggcac cggcgtggcg gcgctgttgg agacggccgc ggcgctgggc
     1021 ccgcggccgc agatcgccaa cgccgtgcgg ttcggtttct ggggcgctga ggaggccgtg
     1081 ctggccgggt cgacgaagta tgtccagagc ctcaacggtg accagctcag cgacatcgcg
     1141 ctctacctcg acttcgacat gctgggctcg ccgaatgccg gtttcttcac ttacgacggc
     1201 gaccagtcag gccaggcgaa cccagatgtc ccggcgaaca ccgtgcccgt cggctcggcg
     1261 ggcatcgagc gcaccatggc cggctacctg aacctggccg ggatccggcc cgccgacctg
     1321 ccgctgggaa aggcgagcga ctacagcccg ttcctcgacg cgggcgtgcc gatcggtggc
     1381 acgaccaccg gtgcggcgca gcgcaagacg gagctgcagg cgcggttgtg gggcggcgtc
     1441 gcgggcgttg cgttcgaccg gaattaccac ctgcccggcg attccgtgga caacgtcaac
     1501 cgcgacgcgc tcgccgttac gggccccgct gtggcgttcg cggtcggcac ctacgcccag
     1561 tcgatcgagg gcgtcaacgg ggtccctacc cgcgagcagc gccgccggca tacgccttag
     1621 cgacgcggct gacgaactga tacacgccgt cctcgtcggg ggcgagcggc cccggcctcg
     1681 cgtgcggcga cgtgagaccg cgctgcaccg actcgcacgc ggcccagtcc tgacggttgg
     1741 tgaggtccca gaagtcgatg gcatacgacg ggtcgaagcc cggcttctcg gcgacctccg
     1801 gtgggaacgc ccatgcgcat tcgacgtggg tgcggtcgac gtccagcggg gtcatgaggt
     1861 gggtcatcac gtagtcgggg tgcaggctga ccaacaggtt cgggtagccg acgacataca
     1921 tcaccgagcg cagttcgtgc tcggagagac ccttgatcgc gacaccgccg ctggcgccgg
     1981 acagcgacat cgtctcggcg ccttcgatga tcgacatcca gccgcccatc cacgagcctt
     2041 cggtatccag gttctcgcca ctggtcggcg ggctgatccg ggacagctcc gggtgaatcg
     2101 tcgagcagtg gtagcactcc tggtagttct cggcgatgac cttccagttg gtcgccaact
     2161 cgtaggagtg ccggtccacc accgtcagat cctcgggccg atacggcgcg acgacgtcct
     2221 cccagccggc cacgtgttcg gagaactcgg cgtcctcgcc gctcggatcg acgaacagcc
     2281 agccgtgcca gttgaccagc cgcagctcgg caagcccgaa ctcgcctgcg tcgaaaccgt
     2341 cgacgtcctt gaagccgggc gcgttgcgca cgctgccgtc cagccggtac gaccaggagt
     2401 ggtacgggca gacgatgctg cggccccgcg tggtctggtt gcatgccagc agttcgtggc
     2461 cgcggtggcg gcaggtgttg gcgaacgcgc gcacggtgtc ctcgccccgc accagcagca
     2521 ccgcgttggc gccggtgccg accgccttct gagctccgac ggtggccagg tcggaggcgt
     2581 ggccg
//
LOCUS       DASYLX010000698         6925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3440014,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000698 DASYLX010000000
VERSION     DASYLX010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440014"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VGP27_16450"
     CDS             complement(<1..1428)
                     /locus_tag="VGP27_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV7852925.1"
                     /translation="MTASGTVRVQTPIAGLVALALRDPCLQEVTSRAADRPADLTLIG
                     PASARLFAACALAEAGPLLVVTATGREADDLTAELRGVYGDSAALFPSWETLPHERLS
                     PGVDTVGARMMVLRRLANPDDERLGPPLRVVVTTVRSLVQPMAPDLADIEPVTLTRGE
                     DADFDGVIHRLVELAYTRVDMVGKRGEFAVRGGILDLFPPTAEHPVRVEFWGDEVSEI
                     RMFSVADQRSIPDIDVDTVFAVPCSELLLTDDVRVRAFELAAEHPRTGTEVPGSVPDM
                     LAKLGEGIAVDGMESLLPLLRPAELSLLSDHLPAHTPLLICDPEKIRTRAGDLIKTGR
                     EFLEASWSTAAVGGDAPIDIEALGASGYLNLEDVIAGARSGGHPWWTLSQLTAYAGGE
                     DEGETAIELDIRPSPSTRGQQFGVDEIFAMLRAHVATGGSAAVVTPGTGTAHRVCEQL
                     ADAETPASMLEPGETPKPGVVGVIKG"
     gene            complement(1503..2105)
                     /locus_tag="VGP27_16455"
     CDS             complement(1503..2105)
                     /locus_tag="VGP27_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7852926.1"
                     /translation="MTGAERRHQLIDIARSLFAERGYEGTSVEEIAQRANVSKPVVYE
                     HFGGKEGLYAVVVDREMSALLDGITSSLTRTTNNRSRLRIERVTLALLTYVDERTDGF
                     RILIRDSPAAITSGTYSTLLNDAVNQVSSILAGDFSRRGLDPEMAPLYAQALVGSVSM
                     TAQWWLDVREPKKEVVAAHLVNLCWNGLMHLEDDPPLLDE"
     gene            2192..2263
                     /locus_tag="VGP27_16460"
     tRNA            2192..2263
                     /locus_tag="VGP27_16460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Gln,seq:ttg)
     gene            2285..3757
                     /gene="glmU"
                     /locus_tag="VGP27_16465"
     CDS             2285..3757
                     /gene="glmU"
                     /locus_tag="VGP27_16465"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV7852927.1"
                     /translation="MSEHGQSAVLVLAAGAGTRMRSDIPKVLHTLAGRSMLAHVLHSI
                     AKVAPQQLVVVLGHDRDRITPAVAELAESLGRQIDIAVQDEQHGTGHAVGCGLTALPA
                     DFAGTVVVTSGDVPLMDADTLAELIAAHSAATAAATVLTTTLPDPTGYGRVLRTQDGE
                     VIGIVEEVDATRSQQAISEVNAGVYAFDIEVLRSALNRLRPHNAQQELYLTDVISIIR
                     QDGLIVRAQHVDDAALVAGINDRVQLSELAAELNRRIVAAHQRAGVTVVDPASTWIDV
                     DVTVGRDTVIQPGTQLLGTTRIGGRCEIGPDTTLTDVAVGEGASVVRTHGSVSAIGDG
                     AVVGPFTYLRPGTVLGDVGKLGAFVETKNATLGAGTKVPHLTYVGDADIGEHSNIGAS
                     SVFVNYDGETKRRTTIGSHVRTGSDTMFVAPVTIGDGAYTGAGTVVRDDVPPGALAVS
                     AGPQRNIEGWVERKRPGSKAAEAARRANESSEGADRDDDG"
     gene            3866..4846
                     /locus_tag="VGP27_16470"
     CDS             3866..4846
                     /locus_tag="VGP27_16470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV7852928.1"
                     /translation="MGTEWTDNRKNLMLFSGRAHPELAEQVAKELDVPVTAQTARDFS
                     NGEIFVRFDESVRGCDAFVLQSHPAPLNKWLMEQLIMIDALKRGSAKRITAILPFYPY
                     ARQDKKHRGREPISARLVADLFKTAGANRILTVDLHTDQIQGFFDGPVDHMRAQNLLT
                     GHIAENYHDHDMVVVSPDSGRVRVAEKWADALGGVPLAFIHKTRDPLVPNQVKSNRVV
                     GDVRGKTCILTDDMIDTGGTIAGAVDLLHQDGAKDVIIAATHGVLSDPAPQRLAECGA
                     REVIVTNTLPIGEEKQFPQLTVLSIAPLLASTIRAVFENGSVTGLFDGSA"
     gene            4846..5223
                     /gene="arsC"
                     /locus_tag="VGP27_16475"
     CDS             4846..5223
                     /gene="arsC"
                     /locus_tag="VGP27_16475"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922881.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEV7852929.1"
                     /translation="MTDERLARGNNSTESVIYHNPRCTTSRKTLELLRDNGIEPTIIQ
                     YLKTPPSRAELVKLINDAGIDVRTAVRRREPLYAELNLADADDDELLDAMAAHPILIQ
                     RPFVVTPRGTRLARPLDSVREVL"
     gene            5250..5867
                     /locus_tag="VGP27_16480"
     CDS             5250..5867
                     /locus_tag="VGP27_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEV7852930.1"
                     /translation="MSLAVAAAGCGAQSPDYTSLLTTTTSAKPTETTTPVPISQYLES
                     AGVVGQPVAPDKLTDLIVTMPSPKGWAQYSNPRFAPGTRVIAKGDTYPTAMLMVFQLS
                     GGDFDAKKAIEHGNADAELSENFKKLNASLDNFKGFPSSMIEGSYDLNGRRMHSYNRV
                     VIATGAAPANQRYLIQFTVTGYADKARDEASDIEAVISGFNVAKK"
     gene            5922..6791
                     /locus_tag="VGP27_16485"
     CDS             5922..6791
                     /locus_tag="VGP27_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7852931.1"
                     /translation="MSGWTAADLPSFTGRSVIVTGANSGLGEVTARELARVGAKVILA
                     VRNTGKGDAAASGMTGDVEVRKLDLQDLASVRAFADGVDAVDVLVNNAGIMAVPYAQT
                     VDGFESQIGTNHLGHFALTNLLLPKISDRVVTVSSMVHLMGYISLKDLNWKSRPYLAW
                     PAYGQSKLANLLFTSELQRRLSAAGSALKAHAAHPGYSETNLQGKTGNPIGTRFIGFV
                     TPLVGTDADFGARQTLYAASQELPGDSFIGPRFVSRGRTGPTLRSPLARDAKRAAALW
                     ELSEQLTGTKFAL"
BASE COUNT         1198 a         2382 c         2281 g         1064 t
ORIGIN      
        1 acccttgatg acgccgacga cacccggctt gggcgtctcg cccggctcca acatcgacgc
       61 gggcgtctcg gcgtcggcca gctgctcgca gacccggtgc gcggtgccgg tgccgggtgt
      121 gacgacggcc gccgatcccc cggtcgcgac gtgcgcgcgc agcatggcga agatctcgtc
      181 gacgccgaac tgctggccgc gcgtggacgg tgacgggcgg atatccagct cgatggccgt
      241 ctccccctcg tcctctccgc ccgcgtacgc ggtgagctgc gaaagagtcc accacgggtg
      301 accgcccgaa cgggcgcccg cgatgacgtc ctcgaggttc agatagcctg acgcgccgag
      361 cgcctcgatg tcgatgggtg cgtcgccgcc gacggcggcc gtcgaccacg aggcttcgag
      421 gaactcgcgg ccggtcttga tcaggtcgcc ggccctggtg cggatcttct cggggtcgca
      481 gatcagcagc ggcgtgtgcg cgggtaggtg gtcgctgagc agggagagtt ccgcgggccg
      541 cagcagcgga agcagtgact ccatgccgtc gacggcgatg ccctcgccca acttggccag
      601 catgtcgggc acgctgccgg gcacctcggt gccggttctg gggtgctcgg cggcgagttc
      661 gaacgcgcgg acccggacgt cgtcggtcag cagcagttcg ctgcacggca cggcgaacac
      721 cgtgtcgacg tcgatgtccg ggatcgagcg ctggtcggcg accgagaaca tccggatctc
      781 ggaaacctcg tcgccccaga actcgacacg cacgggatgc tcggcggtgg gcgggaacag
      841 gtcgaggatt ccgccgcgca cggcgaactc cccccgcttg ccgaccatgt cgacgcgggt
      901 gtaggccagc tcgaccagcc ggtggatgac gccgtcgaaa tcggcgtcct cgccgcgcgt
      961 gagcgtgacg ggctcgatgt cggccaggtc cggcgccatc ggctgcacca gcgagcggac
     1021 cgtggtgacc acgacccgca gcggcggccc gagccgctcg tcgtcggggt tggcaagccg
     1081 gcgcagcacc atcatccgcg cgccgacggt gtccaccccc ggggacagcc gttcgtgcgg
     1141 cagcgtctcc catgacggga acagcgccgc cgaatcgccg tagacgccgc gcagttcggc
     1201 ggtcagatcg tcggcctcgc ggccggtcgc ggtgacgacg agcagcggcc cggcctcggc
     1261 gagcgcgcag gcggcgaaca gcctggcgct ggcgggtccg atgagagtga gatcggcggg
     1321 tctgtcggct gcacggctag tgacctcctg tagacagggg tcgcgcagcg ccagggcaac
     1381 gaggcccgcg atcggggttt ggacacgaac ggtccccgat gcggtcatga tgattccatc
     1441 ttaggcactt gcgatttcgg cgcgctttcg tgtgtcgagc gccactgcgc acgccgattc
     1501 cgctactcgt cgagcagcgg tgggtcgtcc tccaggtgca tcagcccgtt ccagcacagg
     1561 ttgaccagat gcgcggcgac aacttccttc ttgggctcgc gcacgtcgag ccaccactgc
     1621 gcggtcatcg acaccgaccc gacgagcgcc tgcgcgtaca gcggagccat ttccgggtcg
     1681 aggccgcgcc gggagaaatc cccggccagg atcgacgaca cctggttgac ggcatcgttc
     1741 aacagcgtcg agtaggtgcc cgacgtgatg gcggccggtg agtcgcggat caggatgcgg
     1801 aatccgtcgg tgcgttcgtc gacgtaggtc agcagggcca gcgtgacccg ctcgatgcgc
     1861 agccgcgacc ggttgttggt cgtcctggtc agcgacgagg tgatgccgtc cagcagcgca
     1921 gacatctcgc ggtcgaccac caccgcgtag aggccctcct tgccgccgaa atgctcgtag
     1981 acgacgggct tggagacgtt ggcgcgctgg gcgatctcct cgaccgaggt gccctcgtat
     2041 ccacgctcgg cgaacaacga ccgagcgatg tcgatcagct ggtgacgccg ctcggcaccg
     2101 gtcatccggg cgcggggtac acgcagttcc ttctccggta ctgccacacc ggtcagccta
     2161 gttgcttcga ctaacatcac gggttgatca gtccgtcgtg gtgtaatcgg cagcacctct
     2221 gattttggtt cagatagttc aggttcgagt cctggcgacg gagctccccg atgaggacag
     2281 gcagatgagc gagcacggcc aatccgcggt tctggtgttg gccgcaggcg ccggcacccg
     2341 gatgcgatcc gacatcccga aggtgttgca cacgctggcg ggccggagca tgctcgcgca
     2401 cgtcctgcac tcgatcgcca aggtcgcccc gcaacagctc gtcgtcgtgc tcggccacga
     2461 ccgcgaccgc atcaccccgg ccgtcgccga gctggcggag tcgctcggcc gacagatcga
     2521 catcgcggtg caggatgagc agcacggcac cgggcacgcc gtcggctgcg ggttgaccgc
     2581 tctgcccgcc gacttcgcgg gcaccgtcgt ggtgacgtcc ggcgacgttc cgctgatgga
     2641 cgccgacact ctggccgagc tgatcgccgc ccacagcgcc gcgactgccg cggcgaccgt
     2701 gctgacgacg accctgcccg accccaccgg ctacggccgt gtgctgcgca cccaggatgg
     2761 cgaagtcatc ggcatcgtgg aggaagtgga cgccacccgg tcgcagcagg ccatcagcga
     2821 ggtcaacgca ggcgtgtacg ccttcgacat cgaggttctg cgatccgcgt tgaaccggtt
     2881 gcggcctcac aacgcccagc aggagctcta cctcaccgac gtcatctcga tcatccgcca
     2941 ggacggcctg atcgtgcggg ctcagcacgt cgacgacgcc gcgctggtgg cgggcatcaa
     3001 cgaccgggtg cagctgtccg agctggccgc cgaactgaac cgccgcatcg tggccgccca
     3061 ccagcgcgcg ggcgtgacgg tcgtcgaccc cgccagcacg tggatcgacg tcgacgtgac
     3121 cgtcggccgc gacaccgtca tccagccggg cacccagttg ctgggcacca cccgcatcgg
     3181 cggccgctgc gagatcggcc ccgacaccac cctgaccgac gtcgccgtcg gcgaaggcgc
     3241 gtcggtggtc cgcacccacg gctcggtctc cgccatcggc gacggcgccg tcgtcggccc
     3301 gttcacctac ctgcggcccg gcacggtgct gggcgacgtc ggcaagctcg gcgcgttcgt
     3361 cgagaccaaa aacgccacgc tgggcgccgg caccaaggtg ccgcacctca cctacgtcgg
     3421 cgacgcggac atcggcgagc acagcaatat cggtgcgtcg agcgtcttcg tcaactacga
     3481 cggcgagacc aaacggcgca ccacgatcgg gtcgcatgtg cgcacgggct cggacacgat
     3541 gttcgtcgcg cccgtcacga tcggcgacgg cgcgtacacc ggagcgggca ccgtggtgcg
     3601 cgacgacgtg ccgccgggtg cgctggccgt ttcggcgggc ccgcagcgca atatcgaggg
     3661 ctgggtcgag cgcaagcgcc ccggatcgaa agccgccgag gcggcccgaa gggccaacga
     3721 atccagcgaa ggcgccgacc gtgacgacga cggatagacg tgagccggtc ggtttccgtt
     3781 ctcgttctgg caacccggcc acgcgcgtcg cgcggtgata cgtacgatta ggccgatatc
     3841 gatccccgaa gggcgagggc acacagtggg taccgagtgg accgacaacc gcaagaattt
     3901 gatgctgttc tccggtcgcg cgcacccgga actggccgaa caggtcgcca aggagctcga
     3961 cgtaccggtc accgcccaaa ctgcccgcga cttctccaac ggcgaaatct tcgtccgctt
     4021 cgacgagtcg gtgcgcggtt gtgacgcgtt cgtgctgcag agccatccgg cgccgctgaa
     4081 caagtggctg atggaacagc tgatcatgat cgatgcgctc aagcgcggca gcgccaagcg
     4141 gatcaccgcg atcctgccgt tctatccgta tgcccgtcag gacaagaagc accgcggccg
     4201 cgaaccgatc tccgcgcgtc tggtcgccga cctgttcaag acggccggtg cgaaccggat
     4261 cctcaccgtc gacctgcata ccgaccagat tcagggtttc ttcgacggcc cggtcgacca
     4321 catgcgcgca cagaacctgc tgaccggcca catcgccgag aactaccacg accacgacat
     4381 ggtcgtggtg tcaccggatt ccggccgcgt gcgcgtggcc gagaaatggg ccgatgctct
     4441 gggcggcgtg ccgctggcct tcatccacaa gacccgcgac cccctggtgc ccaaccaggt
     4501 gaagtcgaac cgcgtggtcg gtgacgtgcg tggcaagacc tgcatcctca ccgacgacat
     4561 gatcgacacc ggcggcacga tcgccggggc ggtcgacctg ttgcatcagg acggtgcgaa
     4621 ggacgtcatc atcgccgcga cgcacggcgt gctgtccgat ccggcgccgc agcggctggc
     4681 cgaatgcggc gctcgggaag tcatcgtcac caacacactg ccgatcggcg aggagaagca
     4741 gttcccgcag ctgacggtat tgtccatcgc gccgctgctg gccagcacca ttcgcgccgt
     4801 gttcgagaat ggttcggtga caggtctttt cgacgggtcg gcttagtgac tgacgagcga
     4861 ctcgcgcgag gaaacaacag tacagaatcg gtcatctatc acaatccccg ctgcacgacc
     4921 tcgcgcaaga cactggaatt gctgcgcgac aacggcatcg agccgacgat catccagtat
     4981 ctgaagacgc cgccgtcgcg ggccgaactc gtcaagctga tcaacgacgc aggaatcgac
     5041 gtccggaccg cggtgcgcag gcgtgaacct ctctacgccg agctgaacct cgcggacgcc
     5101 gacgacgacg aactgctcga cgccatggct gcgcacccga ttctcatcca gcgcccgttc
     5161 gtcgtcaccc ccagaggcac gcgcctggcc aggccgctcg actcggttcg cgaggttctg
     5221 tgatccgtca ccgctttgcg gcgactctcg tgtcgctggc cgtcgccgcg gcaggctgcg
     5281 gcgcgcagtc ccccgactac acgtcgctgt tgaccaccac cacgagcgcc aagccgacgg
     5341 agaccacgac gccggttccg atctcgcagt acctggagag cgcgggcgtc gtcgggcaac
     5401 ccgtcgcgcc cgacaagctg accgacctca tcgtcacgat gccgagtccg aaaggctggg
     5461 cgcagtacag caatccgagg ttcgccccgg gcactcgcgt catcgccaag ggcgacacct
     5521 acccgaccgc gatgttgatg gtgtttcaac tcagcggcgg tgacttcgac gcgaagaagg
     5581 ccatcgagca cggcaacgcc gacgccgaac tgtcggagaa cttcaagaaa ctcaacgctt
     5641 ctctcgacaa cttcaagggg ttcccgtcgt cgatgatcga gggcagttac gacctcaacg
     5701 gcaggcggat gcactcctac aaccgggtcg tcatcgccac cggtgcggca cccgccaacc
     5761 agcgctacct gattcagttc accgtgaccg gctatgccga caaggcgcgc gacgaggcgt
     5821 ccgacatcga ggcggtcatc agcgggttca acgtcgccaa gaagtgatcc gcatcgccgc
     5881 gcccgcgggt cgcagaaaat gatccggata gggtgtccgt catgagtgga tggaccgccg
     5941 ccgacctgcc ctcgttcacc ggacgtagcg tcatcgtcac cggagccaac agcggcctcg
     6001 gcgaagtcac cgcacgtgaa ctcgcacgcg tcggcgccaa ggtgatcctg gccgtgcgca
     6061 acaccggcaa gggcgatgcg gcagcctcgg gcatgacggg cgacgtcgag gtccgcaagc
     6121 tggatctgca ggatctggcc tccgtgcggg cgttcgccga cggtgtggac gccgtcgacg
     6181 tgctggtgaa caacgcgggc atcatggcgg tgccctacgc gcagaccgtc gacgggttcg
     6241 aaagccagat cggcaccaat catctcggcc acttcgcgct gaccaacctg ctgctgccga
     6301 agatctccga ccgcgtggtc acggtgtcgt cgatggtgca cctgatgggc tacatcagcc
     6361 tcaaggacct caactggaag tcgcggccgt acctggcgtg gcccgcatac ggccagtcga
     6421 agctggccaa cctgctcttc accagcgagc ttcagcgccg cctgagcgct gccggatcag
     6481 cgctgaaggc acatgccgcc caccccggct actccgaaac caatctgcag ggcaaaaccg
     6541 gcaaccccat cggcacgagg ttcatcggtt tcgtgactcc gctggtgggc actgacgccg
     6601 atttcggcgc gaggcagacg ctatatgcgg cgtcccagga gctgcccggc gacagcttca
     6661 tcggtccacg gttcgtgagc cgcggacgca ccggacccac gttgcgcagc ccgcttgccc
     6721 gcgacgcgaa gcgcgccgcc gccctatggg aactatccga gcagctcacg ggcaccaaat
     6781 tcgcactctg agctgctgat gcgctaacct gtgtcggcgt cacggcgagg gtggcatctg
     6841 ccaccgttat cgacgggaac acgcattgcg tgatcgcgac cctgccgcgg acgaggaaca
     6901 accggcatag aggagcaaca cgcat
//
LOCUS       DASYLX010000699         4670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2999760,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000699 DASYLX010000000
VERSION     DASYLX010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999760"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VGP27_16490"
     CDS             <1..1302
                     /locus_tag="VGP27_16490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV7852932.1"
                     /translation="TVGDIESLPFLEAARQVRHEVGRENCFFLHCSLVPYMAPSGELK
                     TKPTQHSVAALRSIGITPDALILRCDRDVPEPLKNKIALMCDVDIDGVISTPDAPSIY
                     DIPKVLHREELDAYVVRRLNLPFRDVDWTQWNDLLQRVHEPRETVRIALVGKYIDLSD
                     AYLSVAEALRAGGFKHRAKVEMRWVASDDCETASGAAAVLDDVHAVLIPGGFGIRGIE
                     GKIGAIRYARKRGLPVLGLCLGLQCIVIEAARSVGITGANSAEFDPATSDPVISTMAD
                     QRDAVAGDADLGGTMRLGAYPAVLEPNSIVAQAYGTTKVSERHRHRYEVNNAYRDRIT
                     QGGLRVSGTSPDGHLVEFVEYAPDVHPFLVGTQAHPELKSRPTRPHPLFVALVGAALD
                     FKAAERLPVEIPEQRANGAEHSDDMEPVLQDGSREKSEARG"
     gene            1295..1918
                     /locus_tag="VGP27_16495"
     CDS             1295..1918
                     /locus_tag="VGP27_16495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7852933.1"
                     /translation="MAEHDFTTVHSETLYVGKIFALRADDVRMPGGNIARREVIEHYG
                     AVAVVAMDDDANIVMVYQYRHAVGRRLWELPAGLLDMGGEPPHVSAGRELEEEAGLSA
                     QNWRVLVDLVSAPGFSDESVRVYLATGLSDIGRPDAHDEEADLVVRWFSLDEAVQMAL
                     SGEIVNSSAVSGILAAHAARGDYDSLRPVDAEWVDRPTAFVRRQQQS"
     gene            1915..2865
                     /gene="xerD"
                     /locus_tag="VGP27_16500"
     CDS             1915..2865
                     /gene="xerD"
                     /locus_tag="VGP27_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV7852934.1"
                     /translation="MTTSVRPALDEQLQGYLDHLTIERGVAANTLSSYRRDLRRYAEH
                     LGQRGIDDLSKVTETDVSDFLAALRRGDSDSGAAPLSAVSAARSLIAVRGLHKFAAAE
                     GVTELDVARAVKPPTPSRRLPKSLTIDEVLALLEGAGGDREADGPLTLRNRALLELLY
                     STGARISEAVGLDIDDIDTHARSVLLRGKGGKQRLVPIGRPAVTALDVYLVRGRPDSA
                     RRGKGTPAIFLNARGGRLSRQSAWQVLQDAAERAGITAAVSPHTLRHSFATHLLEGGA
                     DVRVVQELLGHASVTTTQIYTLVTVHALREVWAGAHPRAR"
     gene            complement(2897..4240)
                     /locus_tag="VGP27_16505"
     CDS             complement(2897..4240)
                     /locus_tag="VGP27_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7852935.1"
                     /translation="MVSLPSGRQYLRDSEAYEEARRATVWNGLVPNRFPDVIVQAEGV
                     EDVVAAVRQANRDGHRISVCSGGHSWAASHLRDGGLLLDVSRIDHCAVDAEGMVAVVG
                     PGKIGSVLAAELDSQGLFFPAGHCEGICIGGYLLQGGYGWNSRSIGPACESVIGIDVV
                     TADGEQFFCDSETNEDLYWAARGSGPGFFAVVTAFHLRLHPRPPVCGSCLYIYPVEVA
                     DEVFTWARSISPQIDDRVELQIITSRNLPALGLDRPSMVVASPVFAQSEEDATQALSI
                     LGTCPVVDQSLIRVPYATTDLAGWYGAIMSNYPSGHRYVADNMWTSASAEELLPGIHR
                     IIDTMPPPPSHFLMFNWSPSAARESLLYGLEDEFNLALYAVWDDPADDERCIEWPRSN
                     MAAMSHLSSGVGLADENLGRRPAKFATDGNMARLDRIREKYDPEARFNSWMGRVS"
     gene            complement(4516..>4670)
                     /locus_tag="VGP27_16510"
     CDS             complement(4516..>4670)
                     /locus_tag="VGP27_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7852936.1"
                     /translation="ADNMKIPGAPDYRAYMREQQDQHWRSIEHRTHGEYQALLPDNLM
                     ESEYLG"
BASE COUNT          802 a         1536 c         1519 g          813 t
ORIGIN      
        1 accgtcggcg atatcgagtc gctcccgttc ctcgaggccg cgcgtcaggt gcgtcatgag
       61 gtgggccgcg agaactgctt tttcctgcac tgctcgctgg tgccctacat ggcgccgtcg
      121 ggtgagctca agaccaaacc cacccagcac tcggtcgccg cgctgcgcag catcggtatc
      181 acccccgacg cgttgatcct gcgttgtgac cgcgatgtgc ccgagccgct gaagaacaag
      241 atcgcgctga tgtgcgacgt cgacatcgac ggtgtgatct cgaccccgga tgcaccgtcg
      301 atctacgaca tccccaaagt gctgcaccgt gaggaactcg acgcctatgt ggtgcggcgg
      361 ctcaacctgc cgttccgcga tgtcgactgg acgcagtgga acgacctgct gcaacgggtg
      421 cacgagcctc gcgagaccgt gcgaattgcg ctcgtgggca aatacatcga cctttccgac
      481 gcatacctgt cggtggctga ggcgctgcgt gccggcggct tcaaacaccg cgccaaggtg
      541 gagatgcggt gggtcgcttc cgacgactgc gagaccgcca gcggcgccgc ggcggtgctc
      601 gacgacgtgc atgccgtctt gatccccggt ggcttcggca tccgggggat cgagggcaag
      661 atcggcgcga tccgctacgc gcgcaaacgc ggcctgccgg tgctcggtct gtgcctgggc
      721 ctgcagtgca tcgtcatcga ggcggcccgg tccgtcggga tcaccggcgc gaactcggcg
      781 gagttcgatc ccgccacctc cgatcccgtc atctccacca tggccgatca gcgcgacgcc
      841 gtcgccggcg acgcggacct cgggggaacg atgcggctgg gcgcctaccc ggccgtactc
      901 gagccgaatt caattgtggc gcaggcgtat ggaaccacga aggtgtccga gcgacatcgg
      961 caccgctacg aggtcaacaa cgcctaccgc gatcgcatta cccagggcgg gctgagggtc
     1021 tcggggacgt cgccggacgg gcacctggtg gagttcgtcg agtacgcccc tgacgtccat
     1081 ccgttcctgg tcgggactca ggcccatccc gagctgaaaa gccgcccgac gcgcccccat
     1141 ccgctgttcg ttgccctcgt cggtgcggcg ctggacttca aggccgccga gcggttgccg
     1201 gtggagatcc ccgagcagcg agccaacgga gccgagcatt cggatgacat ggagcccgtt
     1261 ctgcaggacg gctccaggga aaaatccgaa gcccgtggct gaacacgact tcaccaccgt
     1321 ccactccgaa accctctacg tcgggaagat tttcgcgtta cgcgccgacg acgtacgaat
     1381 gcccggcggt aacatcgccc gacgcgaagt catcgagcac tacggtgcgg tagcggtcgt
     1441 cgcgatggac gacgacgcca acatcgtcat ggtctatcaa taccgccacg cggtgggcag
     1501 acggctgtgg gaactgcccg cagggctcct ggacatgggc ggcgagccgc ctcacgtctc
     1561 ggcggggcgc gaactggagg aagaggccgg gttgtccgcg cagaactggc gcgtcctggt
     1621 cgacctcgtc agcgcacccg gcttcagcga cgagagcgtc agggtgtacc tcgccacagg
     1681 gttgtccgac atcggcagac cggacgccca cgacgaggag gctgatctgg tggtccggtg
     1741 gttctccctc gacgaggcgg tgcagatggc gttgagcggc gagatcgtca acagcagcgc
     1801 ggtgagcgga attctcgccg cacacgccgc tcgaggcgat tacgactcgt tgcgaccggt
     1861 cgacgccgag tgggtcgacc ggccgaccgc gttcgtccgc cggcagcaac agtcatgacg
     1921 acctcggttc gtcccgctct cgacgagcag cttcagggtt acctcgacca cctgacgatc
     1981 gaacgtggcg tcgccgcgaa caccttgagc tcctaccgcc gggatctgcg tcgctacgcc
     2041 gagcacctcg gccagcgcgg catcgatgac ctgagcaagg tcaccgaaac agacgtgagc
     2101 gacttcctgg cggcgctgcg tcgcggcgat tccgacagcg gcgccgctcc gctgtcagcc
     2161 gtgtcggccg cacggtccct gatcgcggtt cggggcttgc acaagttcgc cgccgcggag
     2221 ggcgtcaccg aactcgacgt ggcgcgcgcc gtgaagccgc cgacacccag caggcggttg
     2281 cccaagagcc tgaccatcga cgaggtgctg gccctgctgg agggggcggg cggtgaccgt
     2341 gaggcggacg gcccgctgac gctgcgcaac cgagcgctac tcgaactgct gtactcgacc
     2401 ggcgcacgga tctctgaggc cgtcggtctc gacatcgacg acatcgacac ccacgcccgt
     2461 tcggttttgt tgcgcggcaa gggcggaaag cagcggctgg tgccgatcgg ccgtcccgcg
     2521 gtcaccgcac tcgacgtgta cctcgtccga ggtcgtcccg actcggcccg acgcggtaag
     2581 ggcacgcccg cgatcttcct caacgcacgc ggcggacggc tgtcgcggca gagcgcatgg
     2641 caggtgttgc aggacgccgc cgagcgggcg ggcatcaccg ccgcggtgtc tccgcacacc
     2701 cttcggcatt cgttcgccac ccatcttctg gagggcggcg cggatgtccg cgtggtacag
     2761 gaactgctgg gacatgcgtc ggtcaccacc acgcagatct acacccttgt caccgtgcac
     2821 gcgctgcgtg aggtctgggc tggggcccac ccgcgggcgc gttagcgggt gcacaccgcc
     2881 agtgacgcga cccggttcac gacacgcggc ccatccagct gttgaagcgc gcttccgggt
     2941 catatttttc gcggatccga tctagccggg ccatgtttcc gtcggtggcg aacttcgccg
     3001 gacggcggcc aaggttctcg tccgcgagac cgactccgct ggaaaggtgc gacatcgcag
     3061 ccatattgga cctgggccac tcgatgcacc gctcgtcgtc agccgggtca tcccacaccg
     3121 cgtacagagc aagattgaac tcgtcctcca gtccgtacaa caaggattcc cgggctgctg
     3181 acgggctcca attgaacatc aggaagtgcg atggtggcgg cggcatggtg tcgatgatgc
     3241 gatggatgcc ggggagaagc tcctcggcgg acgccgacgt ccacatgttg tcggcgacgt
     3301 aacgatgacc gctcggatag ttgctcatga tggcgccgta ccacccggcc aggtccgtcg
     3361 tcgcatacgg aacccttatc aacgactggt cgacgaccgg acacgtgccc aggatcgaca
     3421 gggcctgcgt cgcgtcctct tcggactgag cgaagaccgg cgatgccacg accatgctcg
     3481 ggcgatcgag cccgagcgct ggcagattgc gcgaggtgat tatctggagt tcgacacgat
     3541 cgtcgatctg tgggctgatg gaacgtgccc aggtgaacac ttcgtcggca acttcgacag
     3601 ggtagatgta caagcagcta ccgcagacgg gcggtcgtgg gtgaagccgt agatggaagg
     3661 cggtgaccac ggcgaaaaat cccgggcccg aaccgcgtgc cgcccaatag agatcctcgt
     3721 tggtttcgct gtcacagaag aattgttcgc cgtcggcggt gacgacgtcg attccgatga
     3781 cgctctcgca cgccggtccg atcgatcggc tgttccagcc gtagccaccc tgcaggagat
     3841 atccaccgat acagatacct tcgcagtgac cggcgggaaa gaacagtccc tgactgtcga
     3901 gttcggctgc aaggacgctg ccgatcttcc cagggccgac gacggcgacc ataccttccg
     3961 cgtcgacagc gcagtggtcg atgcggctga cgtccaggag cagcccccca tcgcggaggt
     4021 ggctcgctgc ccaactgtga ccgcccgaac agacgctgat ccgatgccca tcacgattgg
     4081 cttggcggac ggcggccacg acatcctcga cgccttcggc ttgcacgatg acgtcgggaa
     4141 accggttcgg caccagccca ttccagacgg tggcgcgccg cgcttcctcg tacgcttcgg
     4201 aatcgcgtag gtactgtcgc cctgacggaa gtgacaccat tgactcgaac ccccagaaat
     4261 tcggctggat tgctgccaga tcgggaatct agcgtaattg tcgcgttgtg tagcggtcac
     4321 cggtgaaatc cgaccgcttg acgcgtctta gggtatccgc cgcagcgaac tcccgactgc
     4381 cgaccgagat tctcggccgt ccctgcgcgg cgggcaggtg ctggtctgtt cgagtcgttc
     4441 gccccacttg ccctcgccgg cgatcgttgg tcacgtattt cgcagcggcc gtttcgaata
     4501 gatccagccg tttcgtcatc ccaagtactc ggattccatg agattgtcgg gcaggagggc
     4561 ctgatattcg ccgtgcgtcc ggtgctcgat gctcctccag tgttggtcct gctgctcgcg
     4621 catgtaggcc cggtaatccg gggcgcccgg tatcttcatg ttgtctgcca
//
LOCUS       DASYLX010000700         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2013215,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000700 DASYLX010000000
VERSION     DASYLX010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013215"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..564)
                     /locus_tag="VGP27_16515"
     CDS             complement(34..564)
                     /locus_tag="VGP27_16515"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7852937.1"
                     /translation="MRLPTAVLAAAGSLCCALSGCSVNGGGLNVPTVSRTALQQDIAQ
                     RLADAGEKPESVTCRQDLVGEVGTTAHCEVVVSASNSFEPIVTVTAVDGAAIDYEMAP
                     AVSRRQLEDVVARLVADSRGPQVKAVSCESGLQGTIGAVAHCDLDADGVWTRHTVTVS
                     GVRGLMMTFDLLPPPA"
     gene            656..1537
                     /locus_tag="VGP27_16520"
     CDS             656..1537
                     /locus_tag="VGP27_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852938.1"
                     /translation="MGRQPRLARRFFDRFEPVHAVTYFSPECRAAFDGLGYRGFWMGY
                     FAGRSAPLGIVPAQVVTALFYNFAPSRVAKALPAAWEVAPPGAALRAREDSAVATLRR
                     YGLTDDGVRQAADLAAKAARSAPLDGRPLFAANSALPWPTEPLATLWHATTLLREQRG
                     DAHVAVLASSGLSGRECNVLHAAAGRVPPEMIMRGRDYDEDQWRHHANRLTERGLLDG
                     HGALTDAGRALKQHIEDVTDVLALSALEGLDDAEVEDLFRALTPLARAVVAGGDVPAT
                     TPMGLNRDELDDDSARL"
     gene            complement(1534..1872)
                     /locus_tag="VGP27_16525"
     CDS             complement(1534..1872)
                     /locus_tag="VGP27_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3140 domain-containing protein"
                     /protein_id="HEV7852939.1"
                     /translation="MPDDNSDTRKEFDDAVNMTATELEKWLGTDESKSVGQKSGGSGE
                     STGHESGRHIIKILKSKKSDLSDSDYSHMSKVVGYTKRHLAQRPSGGLEKSAWRYSLM
                     NWGHDPVKKS"
     gene            complement(1880..2089)
                     /locus_tag="VGP27_16530"
     CDS             complement(1880..2089)
                     /locus_tag="VGP27_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEV7852940.1"
                     /translation="MSKEFKKGDKVSWQSHGSTAEGTVEEKITSDTKAAKRTVRASKD
                     DPQYRVRSEQSGNDAVHKPESLKKK"
     gene            complement(2176..>3579)
                     /locus_tag="VGP27_16535"
     CDS             complement(2176..>3579)
                     /locus_tag="VGP27_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7852941.1"
                     /translation="DVCEKLVACFDAYLAGESAADRMHGLVHGDYRLDNMLFGQQGAE
                     RPLTVVDWQTVTWGPAMIDVAYFLGCALPDDIRRDNYDALLRTYHDALGAEPELSLQD
                     VRDGVRGQSFFGVMMAIVSSMLVERTERGDEMFMTMLRRHSQHVLDTDALATLPAPAA
                     PEALQPCAGDEGVHQPGDEPLWSESWYADFVDEGQGIGGWFRLGLTPNQQTAWVNALL
                     CGPDIPTVAVNDFHLALPDDPGVVCTDTVEFTQAATDPLRRYHVSLRGRGQAYDDPSA
                     LLRGESGRPVELSMDLCWTTAGTPYLYRLTPRYEIPCAVSGSVAFDGRTVEVHAVPGQ
                     RDHSWGVRDWWGMDWVWSALHLRDGTHLHGLDLRIPGAPPLGVGYIQPSEGALIELQA
                     VTARETFGDNDLPLTTELTLRPGDVTATVQIKGHAPALLTATDGRVSHFPRAWASVAT
                     VDGRTGVGWLEWNRNVR"
BASE COUNT          558 a         1213 c         1160 g          648 t
ORIGIN      
        1 ggccgtggcc gacgagttct ccgtcgacgc cgattaggcg ggcggcggca gcaggtcgaa
       61 ggtcatcatc agacctctga cgccggacac cgtgacggtg tgccgcgtcc acacgccgtc
      121 ggcatcgaga tcacagtggg ccaccgcccc gatcgtgcct tgcaggcccg actcacacga
      181 cacggctttc acctgcggcc ctctggagtc agcgaccagc ctcgcgacca cgtcctcgag
      241 ttgtcgtcgc gacaccgcgg gcgccatctc gtagtcgatc gccgcgccgt cgacggcggt
      301 gaccgtgacg atcggctcga agctgttgct cgcactcacg acgacctcgc agtgggcggt
      361 cgtgcccacc tccccgacca ggtcctgccg gcaggtcacc gactccggct tctcgccggc
      421 gtcggcgagc cgctgcgcga tgtcctgctg cagtgccgtc ctggagaccg tgggtacatt
      481 gaggccgccg ccgttcacac tgcagccgga cagcgcacag cacagcgacc cggccgcagc
      541 cagaacggca gtcggtagac gcacccagcc tcctggcgtt tgctaaattt gcatggctcg
      601 tggatttcga cagacggtat caggagaaac cgaacggagc taattaactg tgaccatggg
      661 caggcaaccg cggctggcac gtcggttctt cgatcggttc gaaccggtgc acgccgtgac
      721 ctacttctcg ccggaatgcc gggcggcgtt cgatggtctc ggctaccgcg gcttctggat
      781 gggttacttc gcgggacgat ccgctccgtt gggaatcgtg cccgcgcaag tcgtgaccgc
      841 gctgttctac aacttcgccc ccagtcgggt ggcgaaggcg ctacccgccg catgggaagt
      901 cgcccctccc ggcgcggcgt tgcgcgcccg tgaggactcc gcggtcgcga cgttgcggcg
      961 atacgggctg acggacgacg gcgttcggca ggccgccgac cttgccgcca aggccgcgcg
     1021 cagcgcgcca ctcgacggca ggcccttgtt cgccgccaac tcagcgctgc catggccgac
     1081 cgaaccgctg gccacgttgt ggcatgccac gacgctgctt cgcgagcagc gaggcgatgc
     1141 gcatgtggcc gtgttggcgt cgtccggact gtccggtcgc gagtgcaacg ttctgcacgc
     1201 cgcggcaggc cgggttccgc cggagatgat catgcgcggc cgggactacg acgaggacca
     1261 gtggcgccac catgcgaacc ggttgaccga gcgtggcctg ttagacggtc acggcgcact
     1321 caccgacgcc ggtcgcgcgc tcaaacagca catcgaggac gtcaccgatg tgctggccct
     1381 cagtgcactc gagggactcg acgacgcaga agtcgaagac ctctttcgag ctctcacccc
     1441 gctcgccagg gcggttgtcg cgggcgggga cgtccctgcc accacaccga tgggcctcaa
     1501 ccgggatgaa ctcgacgacg acagcgcgcg gctctagctc ttcttgacgg ggtcgtggcc
     1561 ccagttcatc agtgagtagc gccatgcact tttctcgagg ccgccagaag gtcgctgcgc
     1621 aagatgacgt ttcgtgtaac cgacgacttt gctcatatgc gagtagtcgc tgtcggacag
     1681 gtccgacttc ttcgacttga ggatcttgat gatgtggcgg ccgctttcat gccccgtcga
     1741 ctcgccgctg ccaccggatt tctgacccac cgacttcgac tcgtcggtgc ccagccattt
     1801 ctccagttcg gtcgccgtca tgttgacggc gtcgtcgaac tccttcctgg tatcgctgtt
     1861 gtcgtcgggc actgtcgggt cacttcttct tcagcgactc gggtttgtgc acggcgtcat
     1921 tgccactctg ctcgctgcgc acccggtatt gcggatcgtc cttggatgcg cgcacggtgc
     1981 gcttggccgc cttggtgtcg ctggtgatct tctcctcgac ggtgccctcg gccgtactgc
     2041 catggctctg ccagctgacc ttgtcgcctt ttttgaattc cttgctcatg cggtcggtct
     2101 acccgctcgc cggatggtcg attcatgtga cctagagcga acgcgaaatg tcataatttg
     2161 cggtgagact cgccgtcatc gcacgttgcg gttccactcc agccaaccga cgccggtccg
     2221 accgtccacg gtggcgaccg acgcccacgc gcgggggaag tggctgaccc ggccgtcggt
     2281 cgcggtgagc agagccgggg cgtgaccctt gatctgcacg gtggcggtga catcgccggg
     2341 cctgagggtc agttcggtgg tcaagggtaa atcgttgtcg ccgaacgttt cccgggccgt
     2401 aaccgcttgc agctcgatca gtgccccttc agacggctgg atgtagccga cgcccagcgg
     2461 tggtgcgccg gggatccgca gatcgagacc gtgcagatga gttccgtcgc gcaggtgcag
     2521 agcgctccac acccagtcca tgccccacca gtcgcgcact ccccacgaat ggtcgcgctg
     2581 tcccggtacc gcatgcacct cgaccgtcct gccgtcgaag gcgacactgc ccgacaccgc
     2641 gcacgggatc tcgtatctgg gtgtcaggcg gtagaggtac ggggtgcccg ccgtcgtcca
     2701 acacagatcc atcgacagct caaccggtcg ccccgactcc cctcgcagca gtgcggacgg
     2761 gtcgtcatac gcctggccgc gaccgcgcag gctgacgtga tagcgccgca acgggtcggt
     2821 ggccgcctgc gtgaactcga cggtgtcggt gcagacgaca ccgggatcgt cgggcagcgc
     2881 caggtggaag tcgttcacgg cgacggtcgg aatatcggga ccgcacagca gcgcgttcac
     2941 ccatgccgtc tgttggttgg gggtcaggcc caaccggaac cagccgccga tgccctgccc
     3001 ttcgtcgacg aagtcggcgt accagctctc gctccacagt ggctcgtcgc cgggttgatg
     3061 cacgccctcg tcgccggcgc agggctgcag cgcttcgggc gccgcgggag cgggcagcgt
     3121 cgccagcgcg tcggtgtcga gcacatgctg actgtgcctg cgcaacatgg tcatgaacat
     3181 ctcgtcgcca cgctcggtgc gctcgacgag catcgaggag acgatcgcca tcatcacccc
     3241 gaagaaactt tggccgcgaa cgccgtcgcg aacgtcctgc aggctgagtt cgggctccgc
     3301 gcccagcgca tcgtggtagg tgcgcagcag cgcgtcgtag ttgtctcggc ggatgtcgtc
     3361 gggtagcgcg cagccgagga aataagcgac gtcgatcatc gcggggcccc aggtcacggt
     3421 ctgccagtcg acgacggtga gcggccgttc ggcgccctgc tgtccgaaaa gcatgttgtc
     3481 gagccggtag tcgccgtgca ccagcccgtg catccggtcg gccgccgact ctccagcaag
     3541 gtacgcgtcg aagcaggcga ccagcttctc gcacacgtc
//
LOCUS       DASYLX010000701         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2670295,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000701 DASYLX010000000
VERSION     DASYLX010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670295"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGP27_16540"
     CDS             <1..492
                     /locus_tag="VGP27_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV7852942.1"
                     /translation="IVAPAPADELLGHYRDAEAASGVGWNYLAAINLIETRLGSVVGP
                     SSAGAQGPMQFLPSTFAGYGEGGDIYSPRDSIMAAGRYLAANGFATNRDQAIYRYNHA
                     AQYVRAVNDYAGVLAADPAAFTGFYRWDVYVKTTAGDLLLPIGYASDAPIPVAEYLAS
                     HPQ"
     gene            complement(511..1356)
                     /locus_tag="VGP27_16545"
     CDS             complement(511..1356)
                     /locus_tag="VGP27_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7852943.1"
                     /translation="MKPTDNHVHTRWSWDTASSSSMQLACARAVELGVPGLAFTEHVD
                     FTEWSLEDSDEHAGLKDQPPARVGHRPRVAPLDVEGYLADLARCRDEFVDLRILSGIE
                     AGEPHLFPASVAGVLAAGNFERVLGSLHGIVVDGTLTGISNGLFREIDAHEVMRRYFA
                     ELVVLVGGSSVFNVLAHCDYARRYWPTGRVGAYDESDFEEEYRTVFRSLASSGRALEI
                     NTRSPMASVSQLRWWWEEGGDAVSFGSDAHQPLRVGEHFDVAVDIAEAAGFRAGRDRF
                     DFWRR"
     gene            1435..>1717
                     /locus_tag="VGP27_16550"
     CDS             1435..>1717
                     /locus_tag="VGP27_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852944.1"
                     /translation="MAGSTDRVTRFAADLLDSAAAEGARQSRSAKQQLDHWARVGREV
                     SSQDTAARRRVEAALTGDLELLELNVEEGVVFNAEIAAAIQENLAESNYG"
BASE COUNT          329 a          590 c          539 g          259 t
ORIGIN      
        1 atcgtggctc cggctccggc ggacgaactg ctcggtcatt accgcgacgc agaagcggcc
       61 tctggtgtcg gctggaacta cctcgcagcg atcaacctga tcgagacgcg gctcggcagc
      121 gtcgtcggcc cgagcagcgc aggcgcccag gggcctatgc agttcctgcc gtcgacgttc
      181 gccggctacg gcgaaggcgg cgacatctac tcgccgcgcg acagcatcat ggccgccggc
      241 cgctacctcg ccgccaacgg ctttgccacc aaccgcgatc aagcgatcta tcggtacaac
      301 catgcggcgc aatacgtccg ggcggtcaac gactatgcgg gggtgctggc ggccgatccc
      361 gcggcgttca ccggcttcta ccgttgggac gtctacgtga agacgaccgc gggggatctc
      421 ctgctgccga tcggttacgc atcggacgcc ccgatcccgg tcgcggagta cctcgcgtcg
      481 cacccgcagt gacgccgtcg acgtccaggc tcaacgccgc cagaaatcga agcgatcgcg
      541 gccggccctg aagccggccg cttcggcgat atccaccgcc acgtcgaagt gctcgccgac
      601 ccgtaacggc tgatgcgcgt cgctgccgaa cgagaccgcg tcgccgccct cctcccacca
      661 ccaccgcaac tgggagacgg acgccatcgg actgcgcgtg ttgatctcca gcgcccgccc
      721 cgaagacgcc aacgaccgga acaccgtccg gtactcctct tcgaagtccg attcgtcgta
      781 ggcgcccaca cgtccggtcg gccagtagcg tcgtgcgtaa tcacagtggg ccagcacgtt
      841 gaacaccgac gaaccgccga cgaggaccac cagttctgcg aagtagcggc gcatcacctc
      901 atgcgcatcg atctccctga acagaccgtt gctgatgccg gtgagcgtgc cgtcgacaac
      961 gatcccgtga agactgccga gcacgcgttc gaaattgccg gcggccagca cgccggccac
     1021 gctggcaggg aagaggtgcg gctcaccggc ctcgatgccg gagagaatgc gcaagtccac
     1081 aaactcgtcg cggcagcgcg ccaagtcagc cagatatccc tcgacgtcga gcggcgcaac
     1141 gcgcgggcgg tgacctacgc gagccggagg ctggtctttc aaacccgcgt gctcgtcgga
     1201 gtcctcgaga ctccattcgg tgaaatcgac gtgctcggtg aacgccaaac caggaacacc
     1261 cagctcgacg gcgcgcgcgc aggcaagctg catcgacgag ctcgacgcgg tgtcccacga
     1321 ccaccgagtg tggacgtgat tatcggtggg cttcatcgtc accgatcgtg gcacatacaa
     1381 agggcgtagc acattgctac acttgtagca tattgctaca acctggaggt caagatggcg
     1441 ggaagcacgg atcgcgtcac ccgtttcgca gcggatctgc tcgatagcgc cgccgccgag
     1501 ggtgctcgcc aaagtcgatc ggccaaacag caactcgacc actgggcgcg ggtcggccgc
     1561 gaggtctcca gtcaggacac cgcggcgcgc aggcgggtcg aggcagcgct caccggagac
     1621 ctggagctgt tggagctcaa cgtcgaagaa ggcgtcgtct tcaatgccga gatcgccgcc
     1681 gcaatccagg agaacctggc ggagtccaac tacggcg
//
LOCUS       DASYLX010000702         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_916278,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000702 DASYLX010000000
VERSION     DASYLX010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916278"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VGP27_16555"
     CDS             complement(<1..492)
                     /locus_tag="VGP27_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEV7852945.1"
                     /translation="MNTELTPLLTAAVIFGWASGVLFTVVGVYLSIRHRRLHPLLLLC
                     ISAISFSWIEAPYDWAMYAQFPPALPRMPSSWPLNMTWGGLPSSVPVGYIGYFVLPAV
                     IGAALGRRLSGHFGWRRPITLLTVGLVVGFCWALVFNAGFGARLGVFHYAYVIPGLGL
                     FEGT"
     gene            693..1358
                     /locus_tag="VGP27_16560"
     CDS             693..1358
                     /locus_tag="VGP27_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852946.1"
                     /translation="MPQRQSPANRSVVKTSIHAARRPRGEPRRLLLDAARELFARKDY
                     RSTTTREIAESAGVTEYLLFRHFGSKAGLFREALILPFTRIVDEFGRTWQELVPEKTD
                     EDELARHFVEQVYDVIVEHRGLVLTLVASEAFSEEEIRDAGIADIRRALAVLSQISVE
                     GMQLRGLRSSRPDMPAHSTMAMIVGMVALRSTFFGTRPPSRDAIVDELVQATLHGFLH
                     RGD"
     gene            complement(1398..2834)
                     /locus_tag="VGP27_16565"
     CDS             complement(1398..2834)
                     /locus_tag="VGP27_16565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7852947.1"
                     /translation="MEQLSWTDDMMLRAEKPETPMQIQMLLIYDQSTAQGGSVTFKRI
                     LSEIDARLHLAPTFRRRLTELPGGLHMPYWVDDPDFDLEYHVRHIALPQPGDWRQLCI
                     QIARIHGRQLDLRRPPWEMTVIEGLNSVPGVPKGSFAVSLKLHHCVVDGMESVELMAA
                     MHDLASDSPRPAAPEKPWKAGALPSTTSLVSRTAINGVLHPMRAGKVLVPSAFNALRH
                     LAELPGRLAGGVAATLAPGSGSLFAPSTRFNNAVTPHRVFEARFHDLADFQRIKACVP
                     NATINDVALAYVGGTLRRYLDGHDELPDESLVAACPMSIRSSGDSGTGGNRLFGRLQA
                     LGTTIADPLERLAAIAAETSVFRNTSDSSRSARLMELVGTVPTTLLGLTVKAASAVPL
                     SGPTIANTTVSNVPGPADPVFFAGARLLRVTGLGPLIGGMNLFHVVASYNGTVSVGVT
                     ADRSALPDPSHYADCMQSAFQEMLSAVR"
     gene            complement(3135..3962)
                     /locus_tag="VGP27_16570"
                     /pseudo
     CDS             complement(3135..3962)
                     /locus_tag="VGP27_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            3948..>4770
                     /locus_tag="VGP27_16575"
     CDS             3948..>4770
                     /locus_tag="VGP27_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7852948.1"
                     /translation="MDHVVVTGAGRGLGRHHALLLASRGARVVVNDVGGSVTGGGSCG
                     DPANDVAQQIKDIGGEAVADTNSVTTPEGGQAIIDTALNAWGRVDILVNNAGTVADAA
                     FHDMTEERLDPLLDVHLKGAFFVTRPAWKVMRHQHFGRIVNTCSAAGILGAPRMSNYG
                     AAKTGLIGLTRVLAAEAAGLDIKVNAVAPVAATRMMNYSLDSAAEANNAPRARGTSTE
                     AEELMRPFLDRLDPGLVSPAVAFLAHAECPVSGEIYTVGGGQICRFFIGRTKGYYN"
BASE COUNT          904 a         1524 c         1536 g          806 t
ORIGIN      
        1 agtcccctcg aacagcccca gcccggggat cacgtaggcg tagtgaaata cgccgaggcg
       61 tgcaccgaaa cccgcgttga acaccaaggc ccagcagaag ccgacgacga ggcccacggt
      121 cagcagcgtg atcggcctac gccaaccgaa atgcccactc agccgtcggc cgagtgccgc
      181 gccgatcacg gcaggcagca cgaagtagcc gatgtagccc acgggcactg acgagggcag
      241 cccgccccat gtcatgttca acggccacga cgagggcatg cgcggcagtg cgggcggaaa
      301 ctgcgcgtac atcgcccagt cgtagggcgc ctcgatccac gagaacgaga tcgccgagat
      361 gcacaggaga agaagtgggt gcaggcggcg gtggcgaatg ctcagataca ccccgacgac
      421 ggtgaacagg acgccgctgg cccagccgaa gatcacggcg gccgttagca agggcgtcag
      481 ctcggtgttc atcgatcgcg ttcgtcactc gcgcggccgc gcgcttccgg gtccaagtat
      541 tggacgacgg cgaagatgac gacaatcagc ccgaacagcg tgccgagcat gatgagctga
      601 cccacagccg gcccctttcg cgacgtccgc cgatcggtaa tagtggacac tattaaaagt
      661 ggtttggtca aggccgacta tcgtcaagaa ccgtgccgca gcgacagtcg cccgcaaacc
      721 ggagcgtggt gaagacgtcg attcacgccg cgcgccggcc ccggggcgag ccgcgcagac
      781 tgctgctcga cgccgcccgc gaactgttcg cccgcaagga ctaccgcagc acaacgacgc
      841 gcgaaatcgc cgagagcgca ggcgtcaccg agtaccttct gttccggcac ttcggttcca
      901 aggcagggct gttccgtgag gcgctaattc ttccgttcac gagaatcgtc gacgagttcg
      961 gtcgcacgtg gcaggaactc gttcccgaga agaccgacga agacgaactg gcccgccatt
     1021 tcgtagaaca ggtctacgac gtcatcgtcg agcatcgggg tctggtcctg acgcttgtcg
     1081 cctcggaggc gttcagcgag gaggaaatcc gcgatgcggg catcgccgac atcaggcggg
     1141 cgctcgcggt actaagtcag atcagcgtcg agggcatgca gttgcggggg ctgcgatcga
     1201 gccggccgga catgcccgcg cactcgacga tggcgatgat cgtcggcatg gtcgcgctgc
     1261 gctcgacctt cttcggaacg aggccgccct cgcgtgacgc gatcgtcgac gaactcgtgc
     1321 aagccacgct gcacggcttc ctgcaccgcg gtgactgacg tcgagcatcg gacaaataag
     1381 aaccatgcgt gagcggatca cctgacggcg ctgagcatct cctggaacgc gctttgcatg
     1441 cagtcggcgt agtggctcgg gtcgggaagc gcactgcggt ccgccgtcac cccgaccgag
     1501 actgttccgt tgtagctcgc gacgacatgg aaaagattca ttccgccgat cagtgggccc
     1561 agaccggtga ccctcaaaag gcgagcaccg gcgaagaaaa cggggtcggc cggccccggc
     1621 acgttactca ctgtggtgtt cgcgatggtg gggcccgaca atggcacagc gctcgcagcc
     1681 ttcaccgtca gtcccagcag cgtggtgggt accgtaccta ccaactccat cagccgggcg
     1741 ctgcgtgagg aatcggaagt gttgcgaaag acagaagtct ccgcggcaat cgccgccaac
     1801 cgttcgagcg ggtcggcaat cgtcgtcccc agtgcctgca acctgccgaa cagtcgattg
     1861 ccgccggtac cgctgtcgcc gctggaccgt atcgacattg ggcaggcggc caccaggctc
     1921 tcatcgggca gctcatcgtg gccgtcgaga taacggcgga gcgtgccgcc aacataggca
     1981 agtgccacgt cgttgatggt cgcgttcggc acacaggctt tgatccgctg gaagtcggcg
     2041 aggtcgtgaa accgggcctc aaacacccgg tgcggtgtga ccgcgttgtt gaagcgcgtg
     2101 cttggagcga agagggagcc cgatccggga gccagcgtcg ctgcgactcc gccagccagt
     2161 ctgcccggaa gctccgccaa atgacggagc gcgttaaaag cactggggac caagactttg
     2221 cccgcgcgca tggggtgaag cactccgttg atcgcggtcc gcgagacaag gcttgtcgtt
     2281 gacggcagcg ctcctgcttt ccaaggcttc tccggcgcag cgggacgtgg gctgtcggag
     2341 gcgaggtcat gcatggcggc catcaactcg acgctttcca tcccgtcgac gacgcagtgg
     2401 tgcagcttga gtgagaccgc aaacgatccc ttcggcacgc ccgggacgga gttcagaccc
     2461 tcaatgaccg tcatctccca cggcggtcgg cgaaggtcga gctggcgtcc gtggattcgc
     2521 gcgatctgta tgcacagctg acgccagtcc cccggttgag gcaaggcgat atgtcgcacg
     2581 tgatattcga gatcgaagtc cgggtcatca acccagtacg gcatgtgcag gccgccaggc
     2641 agctcggtca ggcgccgccg aaaagtcggc gccaggtgca gtcgcgcatc gatctcactc
     2701 aggatgcgct tgaacgtgac gctgccgcct tgtgccgttg actggtcata aatcagaagc
     2761 atctggattt gcatcggggt ctcgggcttt tcggcgcgca acatcatgtc gtcggtccag
     2821 ctcagctgct ccatctccca ccccgttcgc cgcagtcccc cgaagtcgac acgcaggacg
     2881 cacctgttcg cagcttatcg gcgaacacgc gccggtcctt agacgaatcc ctcgggagcg
     2941 gcccgcgctg agcgccggtt aaccacagca aagccatgcg acttgggcga cgcactcagc
     3001 gccgcggcgg cgtcgagggc ctagtcgaaa gcgttccaag gtcggcttgc cgcctttagg
     3061 cattgccgaa cgcggccggg cggcggcccc gatgggttcg acatgaccaa gatcacctgg
     3121 caggttcaat ggaatcacca ggtgactggc agctcgtgaa tggtgtagat ctcccgctcg
     3181 tctccgaaac tcagctcctc ctgcgggacc gccaatcgca gtcccggcag tcgacgtacc
     3241 agcgtggcga gcgcgatctg cagctcgaca cgcgccaggt tctgaccgat gcattgatgg
     3301 accccgaagc cgaagccgag atgtccccgc ggattccggt cgatgtcgaa gatgtcgggg
     3361 ttatcgacga aggcggcgtc ccagtttccg gccggcaggt tcatcagcac gcgatcgccg
     3421 gcccgaatca gctgaccgtt gatcgtgagg tcctcgagag ccacccggtc gacctggctg
     3481 tgcacgatgc tcagataacg catgagctct tcgacgatgt tggcgatcag gacggggtcg
     3541 tccgtctccc tcagacgctg cagttgttcc ggatgctgca gcagcgcaac ggttcccagt
     3601 gcgatcatgc ccgctgcggt ttcgtgaccg gcctgcagta agatcacgcc gttcatgacc
     3661 gctgtctcgc gactgagctc acccttggcg acgtaatcgg tcatcagccg gctcatcaag
     3721 tcgtcgccgg gttcgcggtc cttgagttcg gcaagcttcc acatgtagtc gaacatggcg
     3781 ccgattgcgg cgagtttttc gtcgtcggtc gacctggagt cgaggccctt cgtgctctgg
     3841 tgctggaaga actcgtggtc ctcgtagggc actccgagca gcagggaaat caccagcgac
     3901 gggaccggca gggcgaagtc gtggaccagg tcggccggcg ggccgttgtg gatcatgttg
     3961 tcgtcaccgg cgcgggccgg ggtctcggcc gacaccatgc actgcttctc gcctcgcgtg
     4021 gtgcgcgcgt cgtcgttaac gacgtcggcg gctcggtgac agggggtgga tcgtgcggcg
     4081 accccgccaa cgacgtcgca cagcagatca aggacatcgg cggcgaagcg gtcgcggaca
     4141 ccaacagcgt caccacgccc gaaggcggcc aggccattat cgacaccgcg ctcaacgcct
     4201 gggggcgtgt cgacatcctt gtcaacaacg cgggaaccgt cgccgacgcg gcgttccacg
     4261 acatgaccga ggagcggctc gacccgctgt tggacgtcca cctcaagggt gccttcttcg
     4321 tcacccggcc ggcgtggaaa gtgatgcggc atcagcactt cggccgcatc gtgaatactt
     4381 gctcggcggc cggtattctc ggcgcgccgc ggatgagcaa ctacggggct gccaagaccg
     4441 gactcatcgg gctcactcgc gtgctggcag cagaagcggc cggtctcgac atcaaagtca
     4501 acgcggtcgc gcccgtcgcc gccacccgga tgatgaatta ttcgctggac agtgccgccg
     4561 aggccaacaa tgccccgcgc gcacgaggaa catcaacaga ggcagaggag ctgatgcgac
     4621 cgtttctcga tcgactcgac cctgggttgg tgtccccggc ggtcgcattc ctcgcccacg
     4681 ccgagtgccc ggtgtccggc gagatttaca ccgtcggagg cggacagatt tgccggttct
     4741 tcatcggcag gaccaaaggt tactacaacc
//
LOCUS       DASYLX010000703         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3111077,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000703 DASYLX010000000
VERSION     DASYLX010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3111077"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1186
                     /locus_tag="VGP27_16580"
     CDS             176..1186
                     /locus_tag="VGP27_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEV7852949.1"
                     /translation="MREIKRALVTGGSGFLGGHLCARLLAAGAEVTCVDDLSTSAPNA
                     ASVLQDHPGYTFVRHDISEPLDGLVSTATVDTVFHLASPASPIDYQRRPVETMRAGAL
                     GTANALDVAERWGARTVLASTSEVYGDPLEHPQTESYWGNVNPVGPRSVYDEAKRYAE
                     ALTFAYHRARSADVGVARIFNTYGPGMRSDDGRMVPTFCHQALGGEPITINGSGRQTR
                     SLCYVDDTVAALIALGRADCAGPVNIGNPDELTVLRVAELIRELAGSQSPIEFRAAVA
                     DDPQRRCPDIGLARRQLGWWPRVPHHQGLTETVEWFRLTERRQTCESSGSMRSSTTRQ
                     LH"
     gene            1135..>2504
                     /locus_tag="VGP27_16585"
     CDS             1135..>2504
                     /locus_tag="VGP27_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7852950.1"
                     /translation="MRILGINAVFHDSAAALVVDGQIVAAAEEERFTRRKHGKQAVPF
                     ATWELPVHAARWCLAHAGLQPSDIDAVGYSYDPRLMHGGTDDLEGLDRDWEYLRTLYA
                     ERAPRFLQTALPGIDPGALHYVRHHVAHAASASLASPYPDSAVLTTDGRGERTSMLAG
                     EYRDSKLDILATQELPHSLGLFYENLTEHLGFKRSSDEYKVMAMASYGKPRYLEELRE
                     RVYATGDGGFCTQPVDWESFSPRGEHNFDLACSVQRVLEEVLLDLVIWLRAHTDHRNL
                     CLAGGVALNCVANSRIYAESGFDDVWVQPAAGDSGTALGAALAIAGEAGEPIVPMSSA
                     ALGRGWTDDEIGAALTEAAVPHERPRDLAAAVGKALAENQLIGWFQGRSEYGPRALGN
                     RSLLADPRHAENLERLNTVKGREQFRPVAPMVLAEWASEIFSGGPVPSPYMLFVHDVA
                     ATWRSR"
BASE COUNT          415 a          831 c          865 g          393 t
ORIGIN      
        1 cgaattgtcc tcggatccgt tgtccacggc gatgatgggg cacgccgtcg tgttgagcag
       61 ccgcgtgatc gtcgtggaga gctctgccgc ccggttgcgg ctggcgatca cgaacgtcgt
      121 tcgggcggca gccataaccg aggtacgtac cccgttacac gccaaggaaa cgccgatgcg
      181 tgagatcaag cgtgcgctgg tcaccggcgg cagcggcttc ctcggcggcc acctgtgcgc
      241 acgcctgctc gcggcggggg cggaggtgac gtgtgtcgat gacctgtcca ccagcgcgcc
      301 gaacgcggca tcggttctgc aggatcatcc cggctacaca ttcgtcaggc acgacatcag
      361 cgagccgctc gacgggttgg tgtcgaccgc aacggtcgac acggtctttc atctggcgtc
      421 gcccgcgtcg cccatcgatt accagcggcg tcccgtggag acgatgcgcg cgggcgcgct
      481 cggcaccgcg aacgcactcg atgtcgcgga gcgatggggg gcgaggaccg tgctcgcgtc
      541 gaccagtgag gtgtacggtg atccgctcga acacccgcag accgaaagct attggggcaa
      601 cgtcaatccc gtcggcccgc gtagcgtgta cgacgaggct aagcggtacg ccgaggcgtt
      661 gacctttgcc tatcatcggg cgcgctcggc cgatgtcggc gtggcgcgaa tcttcaacac
      721 gtacgggccg gggatgcgct ccgacgacgg gcggatggtg ccgactttct gccatcaagc
      781 gctgggcggc gagccgatca ccatcaacgg cagcgggcga cagacccggt cgctgtgcta
      841 cgtggatgac accgtcgccg cgttgatcgc gctgggtcgc gcggattgcg ccggccctgt
      901 caacatcggc aaccccgatg agctgacggt gcttcgggtc gccgagctca tccgcgaact
      961 ggcgggcagc caatcaccca tcgagttccg cgccgccgtg gcggacgacc cacaacgccg
     1021 gtgcccggac atcggcctgg cacgtcggca gctcggctgg tggccgcgcg tgccacacca
     1081 ccagggcctg accgaaacgg tcgaatggtt ccggctcacg gagaggaggc agacgtgcga
     1141 atcctcggga tcaatgcggt cttccacgac tcggcagctg cactagtcgt cgacgggcag
     1201 atcgtcgccg ccgctgagga ggagcggttc acccggcgca agcacggtaa gcaggccgtc
     1261 ccgttcgcga cgtgggagct gcccgtgcac gccgcccgct ggtgcctggc acacgcaggc
     1321 ttacagccgt cggacatcga cgccgtcggc tactcctatg acccgcggct gatgcacggt
     1381 ggcacagacg atctggaagg tttggaccgc gactgggagt acctgcggac gctgtacgcc
     1441 gaacgggcgc cgcggttcct gcagaccgcg ctgccgggca tcgaccccgg cgcactgcac
     1501 tacgtccgcc atcacgtcgc gcatgcggcg tcggcctcgc tggcgtcgcc gtatccggac
     1561 agcgcggtgc tgacgacgga cggccggggc gaacgcacct cgatgctggc gggcgagtac
     1621 cgggacagca agctcgacat actcgcgacg caggagctgc cgcattcgct cgggctgttc
     1681 tacgagaacc tcaccgaaca tctgggcttc aagcggtcaa gcgatgagta caaagtgatg
     1741 gcaatggcct cctacgggaa gccgcgctac ctcgaggagt tgcgcgagcg cgtgtacgcg
     1801 acgggcgacg gcggcttctg cacgcagccg gtggactggg agtccttcag ccctcgcggc
     1861 gagcacaact tcgacctcgc atgcagcgtg caacgagtcc tcgaggaggt gctgctcgac
     1921 ctcgtaattt ggctgcgcgc ccacaccgac caccgaaacc tttgcctggc agggggagtg
     1981 gcactcaact gtgtggccaa ctccaggatt tacgccgaaa gcggcttcga cgatgtctgg
     2041 gtccagcccg ccgcgggcga ttcgggaacc gcgctcggcg ctgcactcgc catcgcaggc
     2101 gaggcgggcg aacccattgt gccgatgtcg tcggcggcgc tgggccgagg ctggacggac
     2161 gacgagatcg gcgcggcgct gaccgaggcc gccgtccctc atgagcgacc gagagacctg
     2221 gcggcagcag tgggcaaggc attggcggag aaccagttga tcggctggtt ccagggccgc
     2281 tcggagtacg gtccgcgggc gctgggaaac cgctcactgc tcgccgaccc gcgccacgcc
     2341 gagaacctcg aacggctgaa caccgtcaag ggacgcgagc agttccgccc cgtcgccccg
     2401 atggtgttgg ccgagtgggc gtccgagatc ttctccggcg gcccggtgcc cagcccgtac
     2461 atgttgttcg tccacgatgt cgcggcaacc tggcgctcgc gcat
//
LOCUS       DASYLX010000704         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3769859,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000704 DASYLX010000000
VERSION     DASYLX010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3769859"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGP27_16590"
     CDS             <1..663
                     /locus_tag="VGP27_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEV7852951.1"
                     /translation="DRNRPDLSATSRMSAHLKFGTIHPRTMVTDLGRGKGAQAYLREL
                     AFRDFYAAVVNEWPDSVWWNWNRNFDTMKVDEDADAKKVFEAWKVGRTGYPIVDAGMR
                     QLSETGWMHNRVRMIVASFLVKDLHLPWQWGARWFLEQLVDGDMANNQHGWQWAAGTG
                     TDAAPYFRVFNPTTQGAKFDPDGEYVRRWIPELDTDDYPDPIVDHKAERAEALRRYSA
                     IT"
     gene            complement(656..1489)
                     /locus_tag="VGP27_16595"
     CDS             complement(656..1489)
                     /locus_tag="VGP27_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7852952.1"
                     /translation="MAGQFDGKVVFITGAARGQGRSHAVRFAEEGADVIAVDMCGPID
                     SVAYPMATREDLDETVNLVEKTGRRIVAGQVDVRDFDRLKEFVATGVAELGRVDFILA
                     NAGIFPAAGRQRLDMAAFVDAVDVNLKGVYFTIEAALPAMLERGEGGAIVITSSAAGF
                     KSVSTTFATISHGAAGYTAAKHGVIGVMRHYATSLAEKDIRVNSVHPGGVATPMIINE
                     AMGMLVGDQPAFGEAQQPKLSMPPLEPAEVTDAIVYLCGHSGRHVTGVALPLDGGLTL
                     K"
     gene            1569..1823
                     /locus_tag="VGP27_16600"
     CDS             1569..1823
                     /locus_tag="VGP27_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2631 domain-containing protein"
                     /protein_id="HEV7852953.1"
                     /translation="MVSTELDKTDGVDVEDVPSAAWGWSKENHKYIQIGGIVSALFIL
                     AMLRGNHVGHVEDIFVIVFALLVLGVVARDWWLRRRGWTR"
     gene            complement(1827..>2982)
                     /locus_tag="VGP27_16605"
     CDS             complement(1827..>2982)
                     /locus_tag="VGP27_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7852954.1"
                     /translation="GRRELDQLIGEIASTPLDRSRPLWEMYVAEGLADNRIAIIHKVH
                     HVLADGTASANQMARAMAPQSPTEVVGMFEEPDIERTRTALLMAAGRDHVGLIRKLPR
                     LLSETATGVSRVRRRSKERGEHPDLAKNFSPPDCFINHVVTPGRRFATAPLALDDVRE
                     TAKHLGVTINDIVLAMASGALRRLLLRYDGKADAPLIAGVPVSTDPSPERLAGNEFTY
                     ITPSLPVHVSDALQRVELISTSTAIAKENHQLLGPTLLPAWLSYLPPALAPPVFRKQA
                     QRLESASVMNLTISNVRGPRERGVIEGATVSEIYSVGPVVVGSGMNITVWSYVDQLAI
                     SVLTDDRTLDDPHEATDAMLEAFNEIRAAAGITGDLTPVGAALPLAAAAT"
BASE COUNT          493 a          971 c         1006 g          512 t
ORIGIN      
        1 gaccgcaatc ggcccgacct gtctgccacc agccggatgt cggcacacct caagttcgga
       61 accatccacc cccgcaccat ggtcaccgac ctcggccgcg gcaagggcgc gcaggcgtat
      121 ttgcgcgagt tggcttttcg cgacttctac gccgcggtgg tcaacgagtg gcccgacagc
      181 gtgtggtgga actggaacag gaatttcgac acgatgaagg tcgacgagga cgccgacgcg
      241 aagaaggtgt tcgaggcatg gaaagtcggt aggacgggct atcccatcgt cgatgcgggc
      301 atgcgccagc tctccgagac cggctggatg cacaaccggg tgcggatgat cgtggcctcg
      361 tttctggtca aggatctgca cctgccgtgg cagtggggcg cgcgctggtt tctcgagcag
      421 ttggtcgacg gcgacatggc caacaaccag cacggctggc agtgggccgc gggcaccggc
      481 accgacgccg cgccgtactt ccgggtgttc aacccgacga cgcagggcgc aaagttcgat
      541 cccgacggcg aatacgtccg gcgctggatt cccgagttgg acaccgacga ctatccggac
      601 ccgatcgtcg accacaaggc cgaacgcgcc gaagcgctgc gccgctacag cgcgatcact
      661 tgagcgtcag tccgccgtcc aggggcagcg cgacacccgt cacgtgccgg ccggaatggc
      721 cgcacaggta gacgatggca tcggtgacct cggcgggctc cagcggcggc atggagagct
      781 tgggctgctg agcctccccg aaggcgggct ggtcgccgac cagcatgccc atcgcctcgt
      841 tgatgatcat cggtgtcgcc accccgcccg gatgcaccga gttcacgcga atgtccttct
      901 ccgccaatga cgttgcgtaa tgtcgcatca caccgatcac gccgtgcttg gcggccgtgt
      961 agccggccgc gccgtgactg atggtggcga aggtggtgct gaccgacttg aagccggccg
     1021 ccgaactggt gatgacgatg gcgccgccct cgccccgctc caacatcgcg ggtagcgcgg
     1081 cctcaatcgt gaagtacacg cccttcaagt tgacgtcgac ggcgtcgacg aacgcggcca
     1141 tgtccaggcg ctgcctccct gctgccggaa agatgcccgc attggccagg atgaagtcga
     1201 cccggcccag ttcggcgacg ccggtggcca cgaattcctt gagccggtcg aagtcgcgga
     1261 cgtcgacctg cccggccacg atgcgccgac cggtcttctc gacaaggttg accgtctcgt
     1321 cgaggtcctc acgggtggcc atcggatagg cgacgctgtc gatcggtccg cacatgtcga
     1381 cggcgatgac gtcggcgccc tcctcggcga accggaccgc gtggctgcgg ccctgacctc
     1441 gcgccgcgcc cgtgatgaag acgactttcc cgtcgaactg tcctgccatg acggaccaca
     1501 ctacggccgt gcgtagtagg agcatgtaag aatgtcggca gcgctaggcc cgaccgttag
     1561 gagcagccgt ggtgagcacc gagttggaca agaccgacgg tgtcgacgtc gaagacgtgc
     1621 cgtcggcggc ttggggttgg tccaaggaga atcacaagta catccagatc ggcggcatcg
     1681 tctccgcgct cttcatcttg gcgatgctgc ggggcaacca cgtcggacac gtcgaggaca
     1741 tcttcgtgat cgtcttcgca ctgctggttc tcggtgtcgt cgcgcgagac tggtggctgc
     1801 gtcgccgcgg ctggaccagg tagccgtcag gtagccgccg cagccagcgg caatgccgcc
     1861 ccgacgggcg tcagatcgcc cgtgatgcct gcggccgccc gaatctcgtt gaacgcttct
     1921 agcatcgcgt cggtagcttc atgcgggtcg tccagcgtgc ggtcgtcggt cagcacggaa
     1981 atggccagct ggtcgacgta gctccacacg gtgatgttca taccgctgcc cacgaccacc
     2041 ggtccgaccg agtagatctc gctgaccgtc gcgccctcga tgacgccgcg ctcgcgcggg
     2101 ccgcgcacgt tcgagatggt caagttcatg acgctggccg actcgagacg ctgtgcttgc
     2161 ttgcgaaaca ccggtggcgc cagcgcaggc ggaaggtagg acagccacgc cggcaacagc
     2221 gtcggcccga gcagctggtg gttctccttc gcgatggcgg tcgacgtcga gatcagctct
     2281 actcgttgga gtgcgtcgga gacgtgtacg ggcagcgacg gtgtgatgta ggtgaactcg
     2341 ttgcctgcca acctttccgg tgacgggtcg gtgctcaccg gcacgcccgc gatcaacggt
     2401 gcgtcggcct tgccgtcata gcgaagcagt agtcggcgca gcgcccccga cgccatcgcg
     2461 agcacgatgt cgttgatggt gacaccgagg tgcttggcgg tctccctcac gtcgtccagc
     2521 gccagcggtg ccgttgcgaa gcgacgccca ggggtgacga cgtggttgat gaagcagtcc
     2581 gggggcgaga agttcttggc gaggtcggga tgctcgccgc gctccttgga gcggcggcgc
     2641 acccgcgata cgccggtggc cgtctcgctg agcagccggg gcagcttgcg aatgagcccg
     2701 acgtgatcgc gccccgccgc catgagcagc gctgtcctgg tccgctcgat gtcgggctct
     2761 tcgaacatgc cgacgacttc ggtgggactc tgcggcgcca tcgccctggc catctgattg
     2821 gcagacgccg tgccgtcggc gagcacgtga tggaccttgt ggatgatcgc gatccggttg
     2881 tcggcgaggc cctcggccac gtacatctcc cagagcgggc ggctgcggtc cagcggcgtg
     2941 ctggcgatct cgccgatcaa ctggtcgagc tcgcgacgcc ca
//
LOCUS       DASYLX010000705         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2123968,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000705 DASYLX010000000
VERSION     DASYLX010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123968"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VGP27_16610"
     CDS             complement(<1..1008)
                     /locus_tag="VGP27_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7852955.1"
                     /translation="MSHNSLVTKPASGHWTDDYPELGRGPVSLEDCVSPEFYEKEREH
                     VFRKTWLYMGRVERVPKSGSYFTRELRFLNTSIIIVRGKDDVIRAFHNICPHRGNKML
                     WEDDPFEEVQGRAPLLYCRFHGWRYKLDGSLHSATRKDLLLDFDADSCRVPAIQCEVW
                     EGFIFVNLNPHNTEPVRSFLGELAHGIEGYPFQGPHQVYRFKAELQCNWKIFLDGFAE
                     SYHGPYLHASSFGALTAEAREAFDQPNPFTDALAYQLNGPHRMFSFSGEPSRKTPYSK
                     PIECVFEASAAGPWNKKVERGPMPPGLNPTRSDKYGFDSYQFFPNFVLIFGASGFTVH
                     TH"
     gene            1127..2044
                     /locus_tag="VGP27_16615"
     CDS             1127..2044
                     /locus_tag="VGP27_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7852956.1"
                     /translation="MEQNLPFDLDALLVFGKVVENRSLSKAAALLGMPKSTVSRKLTK
                     LESDLGIKLLRKNTHQLTVTDLGEQIYAHAVNILTEANGVRALVEGSRQEPQGELKVA
                     IPVFVGIDYASRVGATFLQRYPHSRLDIRLVDTMVDPIRDGFDVVFGTGPLQDSTLIA
                     RKVFDLELFLCASPDFVRDLDEPITVPTQLNTLPFIDFGFAGPRRLTVSKQKRQHELA
                     PIVRARANNFQVCKQYILGGLGIGAMPTQIICTEELRDGSLVPVLPEWKLEPLEVHMI
                     YPFELSFSTLISAFYEAACDIIVENIARA"
     gene            complement(2016..2645)
                     /locus_tag="VGP27_16620"
     CDS             complement(2016..2645)
                     /locus_tag="VGP27_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV7852957.1"
                     /translation="MSAARTAAGIWLAGATVYLISEAIAAASVPGYSYVADYISDLGS
                     VAIMNIGAFMLHGTLFLAGAVVIARARPTLGWAGWGFVLAAAANAIGNILVGTFRSGA
                     AGATSHINWHVIGAGMAIIGGNTAVIIAGIVSRRFGAPRRYRRASVLIGVVGIGCLLV
                     LVIDGVNGSRLLPVGVLERGSVYSIVIWEIMTGLAIRASRTTRVRCSRR"
     gene            complement(2642..3775)
                     /gene="nagA"
                     /locus_tag="VGP27_16625"
     CDS             complement(2642..3775)
                     /gene="nagA"
                     /locus_tag="VGP27_16625"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV7852958.1"
                     /translation="MLITTDTLLTGRELLRPAWIEVSDGLVAATGAGPAPRTADRELG
                     AVAVVPGFVDTHLHGGGGANFSTAVHTDTATAVDFHRRHGTTTLIASLVTAGPDDLLR
                     QVEALADDVRFGLLDGIHLEGPWLSTLRCGAHQPSLMRDADPAEIDRVLRAGGGAIRM
                     ITLAPERDGAVDAIRQIVDAGVVAAVGHTEATYDQTRAAIDAGATVATHLFNAMRPIN
                     TREPGPVIALLEDPRVTVELITDGVHVDPALYRHVCRSAGPDRVSLVTDSMAAAGMSD
                     GRYRLGPLAVDVVGGVARVAGTETIAGSTATMDHQFRFAVQHGGLPRDDALMAAVRQA
                     SINPARALGFSSSGLTQGAGADLVVLDAQLTVTGVMRRGSWVA"
     gene            complement(3785..>4057)
                     /locus_tag="VGP27_16630"
     CDS             complement(3785..>4057)
                     /locus_tag="VGP27_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV7852959.1"
                     /translation="DLDAVPTHCLTQGLATIMEAGHLILVATGRSKAEAVHHLVEGAV
                     SALWPATILQHHPHVTVLLDDAAARRLQLVDYYRETYLSKPKWQGI"
BASE COUNT          747 a         1334 c         1298 g          678 t
ORIGIN      
        1 gtgcgtgtgc accgtgaagc cggacgcacc gaagatcagc acgaaattgg ggaagaactg
       61 ataggagtcg aagccgtact tgtcggaccg ggtggggttg agcccgggag gcatcggccc
      121 gcgctcgacc ttcttgttcc aagggccggc cgcgctggcc tcgaatacgc actcgatcgg
      181 tttcgagtac ggcgtttttc gagacggctc gccggagaac gagaacatcc ggtgcggacc
      241 gttgagctga tatgcaagcg cgtcggtgaa tgggttcggc tgatcgaacg cctctcttgc
      301 ctcggctgtc agggcgccga atgaggacgc gtgcaggtac ggcccgtggt agctctccgc
      361 gaagccgtct aggaagatct tccagttgca ctgcaattcg gccttgaacc ggtagacctg
      421 gtgcggaccc tggaagggat agccctcaat gccgtgcgcc aactcaccga ggaacgaccg
      481 caccggttcg gtgttgtgcg ggttgaggtt gacgaagatg aagccctccc acacctcgca
      541 ctggatggca ggcacccggc agctgtcggc gtcgaagtcg agcagtagat ccttgcgggt
      601 ggcggagtgc agggagccgt ccagcttgta gcgccagccg tggaaacggc agtacagcag
      661 cggcgcgcgg ccctgcacct cctcgaacgg gtcgtcctcc cagagcatct tgttgccgcg
      721 gtgcggacag atgttgtgga aggcgcggat cacgtcgtcc ttgccgcgca cgatgatgat
      781 cgaggtattc aagaagcgga gctcgcgggt gaaatagctg cccgacttcg gcacccgctc
      841 gaccctgccc atgtagagcc acgttttcct gaagacgtgc tcgcgctcct tttcgtagaa
      901 ctccggcgat acgcagtctt cgagcgacac cgggccgcgg ccgagttcgg gataatcgtc
      961 ggtccaatgt ccgcttgcgg gtttggtcac caggctgttg tgactcatga ggcccacctc
     1021 ctggccgaac gtaacagctg gtgatttgct cgaaacagat gcaaaacctc aacaccctgt
     1081 tgcatatttg gaacgacgcc ggctgacgcg aggagcaaac agcccagtgg aacagaacct
     1141 gcccttcgac ctggacgccc tgctggtatt cggcaaggtg gtggagaatc gcagcctgtc
     1201 gaaggcggcc gcgctgctcg ggatgcccaa gtcgacggtc agccgaaagc tgacgaagct
     1261 ggaatccgac ctcggcatca agctgctgcg caagaacacg caccagctga ccgtcaccga
     1321 cctcggtgag cagatctatg cccacgcggt gaacatcctg accgaggcca acggtgtgcg
     1381 cgctctggtc gagggcagca ggcaggagcc gcagggtgag ctgaaggtcg cgataccggt
     1441 cttcgtcggt atcgactatg cgtcgagggt cggcgccacc ttcctccagc gctatcccca
     1501 ctcacgcctc gacatccggc tggtcgacac catggtcgac cccatcagag acggattcga
     1561 cgtggttttc ggaaccgggc cgctgcagga ctcgacgctg atcgcccgca aggtctttga
     1621 tctggagctg tttctctgcg cgtcaccgga cttcgtgcgg gacctggacg agccgatcac
     1681 tgttccgacg cagctgaata cgttgccctt catcgacttc ggcttcgcag ggccacgcag
     1741 gctgacggtg tccaagcaga agcgccaaca cgagctggca cccattgtgc gggcgcgcgc
     1801 caacaacttt caggtgtgca agcaatacat ccttggcggc ctcggcatcg gcgccatgcc
     1861 cacgcagatc atctgcaccg aagaactgcg tgacggcagc ctcgtgccgg tgctgccgga
     1921 gtggaaactc gaaccgctcg aggtacacat gatctatccc ttcgagctgt ccttttcgac
     1981 gctcatcagc gcgttctacg aggcggcatg cgacatcatc gtcgagaaca tcgcacgcgc
     2041 gtagtccgtg acgcgcgaat agcgagccct gtcatgatct cccaaatgac gatcgagtac
     2101 accgaaccgc gctccagcac tccgacggga agaagccgag acccgttcac accgtcgatg
     2161 accagaacaa gaaggcaccc gatcccgacc acgccgatga ggacgctcgc gcgacgataa
     2221 cgtcgtggtg caccgaaccg gcgactgacg attccggcga tgatcaccgc tgtgttcccg
     2281 ccgatgatcg ccatcccggc accgatcacg tgccagttga tgtgcgaggt tgctccggca
     2341 gcaccgcttc ggaacgtgcc gaccaggatg ttgccgatcg cgttcgctgc cgccgcaaga
     2401 acgaaacccc aaccggccca gcccagagtc gggcgcgcac gcgcgatgac aacggcgccc
     2461 gcgaggaaca acgtgccgtg cagcatgaac gccccgatgt tcatgatcgc cacggacccg
     2521 aggtcgctga tgtagtcggc gacgtagctg taaccgggaa cgctcgcggc tgcgatggcc
     2581 tcgctgatca ggtacacagt cgcgcccgcc aaccagatac cagcggctgt acgcgccgcg
     2641 ctcacgccac ccacgacccc cgccgcatca cgcccgtcac ggtaagttgc gcgtcgagca
     2701 ccaccaagtc ggcgccggcg ccttgtgtca atcccgaaga cgaaaagccc agcgcccgtg
     2761 cgggattgat cgaggcctga cgcaccgcgg ccatcaacgc gtcgtcgcgc ggtaatccgc
     2821 cgtgctgcac ggcgaaccgg aactggtggt ccatcgtcgc ggtgctgccc gcgatggttt
     2881 ccgtgcccgc gacccgcgcc actccgccga ccacgtcgac ggccagcgga ccaagccggt
     2941 atcgaccgtc ggacatgccc gcggccgcca tcgaatcggt caccaacgac acccgatccg
     3001 gtccggcgct gcggcagaca tggcgataca gagccgggtc gacatggaca ccgtcggtga
     3061 tcagttcgac cgtcaccctc gggtcttcca gtaacgcgat gaccgggccc ggttcgcggg
     3121 tgttgatcgg acgcatcgcg ttgaacagat gcgtggccac cgtggcgccc gcgtcgatcg
     3181 ccgcgcgggt ctggtcatag gtcgcctcgg tgtggccgac ggcggccacg acgcccgcgt
     3241 cgacgatctg ccggatcgcg tcgacagcgc cgtcccgttc gggcgccaaa gtgatcatgc
     3301 ggatcgcgcc gccgcccgcc cgaaggacgc gatcgatctc ggctgggtcg gcatcccgca
     3361 tcagggacgg ctggtgcgca ccgcaccgca gcgtggaaag ccacgggcct tccaggtgga
     3421 ttccgtcgag caggccgaag cggacgtcgt cggccaatgc ctcgacctga cgcagcaggt
     3481 catccggacc ggcagtcacc agggacgcga tcagcgtcgt ggtgccgtgt ctgcgatgga
     3541 agtccacggc tgtggccgtg tcggtgtgca ccgcggttga gaagttcgcg cctcccccgc
     3601 cgtgcagatg ggtgtcgacg aaacccggca ccaccgccac cgcgcccagc tcccggtccg
     3661 cggtacgggg cgccggaccc gcccccgtcg cagccaccag gccgtcggag acctcgatcc
     3721 acgcgggccg caacagctct cggcctgtca gcagagtgtc ggtggtgatc agcatgacaa
     3781 tgtctcagat gccctgccat ttcggcttcg agaggtaggt ctcgcggtaa tagtcgacga
     3841 gctgaagccg gcgggcggcg gcgtcgtcga gcagcacggt gacatgcggg tgatgctgca
     3901 ggatggtggc gggccacagc gcgctcaccg cgccctcgac cagatggtgc accgcctcgg
     3961 ccttgctgcg acccgttgcc accaggatca gatggcctgc ctccatgatg gtcgccagcc
     4021 cctgggtcag gcagtgggtg ggcaccgcgt ccaggtc
//
LOCUS       DASYLX010000706         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3440826,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000706 DASYLX010000000
VERSION     DASYLX010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440826"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..>1710)
                     /locus_tag="VGP27_16635"
     CDS             complement(270..>1710)
                     /locus_tag="VGP27_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEV7852960.1"
                     /translation="RFLRESTIFPVRTPHGSYRLAVADPSDTAAIRAAEIVFGTPVEV
                     AVASFEDITTLLDQRLETEGATADEAAKGSTRQSDDDIESLRDLASGAPVVRALNDLL
                     ERAVELRASDIHIEPFRTGLVVRMRVDGLLRAMPAPSGIPPQALISRVKILAGLNIAE
                     RRLPQDGAARVRVSRSELDVRVATMPTQAGESAVIRLLPRDRGLLEMGKLGLATRDEQ
                     IMTRLLALPHGMIVVTGPTGSGKTTTLATMLSILNQPTRKILTIEDPVEYEIPGVNQS
                     QVKPSIGLTFATAMRAFVRQDPDVIMVGEIRDAETAHIAIHAALTGHLVITTLHTETA
                     AAAVPRLIDLGIEGFLLKSTLRAVVAQRLVRVLCDRCKLPHALTVADIGKDPRFAVIG
                     FKAGDVIHEAGGCERCGGTGYRGRNGVFEILEMSDEVRKLVGPQTDSHTIDEAAMAAG
                     MTTMLADAVAKCRAGLTTVPEVFRVTTIR"
BASE COUNT          285 a          560 c          577 g          288 t
ORIGIN      
        1 gatctacatg tcgcgcgaga accgcaagca ccgcgagatc gtcgatgcca tcgagcgcca
       61 cggtccgccg cccggctggc ggggttagac gcgacgaacg cacgctgttg cggtaacggc
      121 ggtgcgctcc ctctccccag cgggagaggg ttggggtgag gggaactagc tctcttgata
      181 gaccgtaacc cctcacccgg attgcttcgc aatccgacct ctcccaatgg gagaggtaag
      241 agccgactgt gcgagctgct cccatcggtt caccggatcg tcgtgacgcg gaaaacctcg
      301 ggcacggtgg tgaggccggc gcggcacttg gcgaccgcat cggccagcat cgtggtcatc
      361 ccggcggcca tcgcggcctc gtcgatggta tgcgaatccg tctgcggccc caccagcttg
      421 cgaacctcat cggacatttc gaggatctcg aacacgccgt tgcggccgcg atagccggtt
      481 ccgccacagc gctcgcagcc gccggcctca tgaatcacgt cgccggcctt gaagccgatc
      541 acggcaaaac gtggatcctt gccgatgtcg gcgacggtca atgcgtgcgg cagcttgcag
      601 cgatcgcaca gtacccgcac caggcgttgc gccaccaccg cccgcagcgt cgatttcagc
      661 aaaaacccct cgatgccgag atcgatcagc cgcggcacgg ccgcggctgc ggtctcggtg
      721 tgcagggtgg tgatgaccag atgcccggtg agtgccgcgt gaatcgcgat gtgcgcggtt
      781 tcggcgtcgc ggatttcgcc gaccatgatc acgtcaggat cctggcggac gaaggcgcgc
      841 atcgcggtgg caaaggtcag gccgatcgac ggcttgacct gcgactggtt gacgcctgga
      901 atttcatatt cgaccgggtc ttcgatcgtc agaatcttgc gggtcggctg gttcagaatc
      961 gaaagcatgg tcgccagcgt cgtcgtcttg ccgctgccgg tcggccccgt caccacgatc
     1021 atgccgtgcg gcagcgccag cagacgggtc atgatctgct cgtcccgggt tgcgaggccg
     1081 agcttgccca tttcgagcag accgcggtcg cgcggcaaca accggatcac cgcgctctcg
     1141 ccggcctgcg tcggcatggt cgcgacgcgg acgtcgagtt cggagcggct gacgcgaacc
     1201 cgtgccgcgc cgtcctgcgg cagccgccgt tcggcgatgt tgaggccggc gagaatcttc
     1261 acccgcgaga tcagcgcctg cggcgggatt cccgacgggg ccggcatggc gcgaagcaat
     1321 ccgtcgacgc gcatgcggac cacgaggccg gtgcggaacg gctcgatgtg aatgtcgctg
     1381 gcgcgaagct cgaccgcgcg ctcgagcagg tcgttgagcg cgcgcaccac gggcgcgccg
     1441 ctggcaagat cgcgcaggct ctcgatatcg tcgtcggatt gccgggtcga tcctttggct
     1501 gcctcatccg cggttgcgcc ttcggtctcg aggcgctgat cgagcaaggt cgtgatatcc
     1561 tcgaacgacg ccaccgcgac ctcgaccggc gtgccgaaca cgatttcagc ggcgcggatc
     1621 gcggcggtat ccgacgggtc ggcgacggcc agccgataac ttccgtgggg cgtacggacc
     1681 ggaaagatcg tcgactcgcg cagaaagcgg
//
LOCUS       DASYLX010000707         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2342861,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000707 DASYLX010000000
VERSION     DASYLX010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342861"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGP27_16640"
     CDS             <1..541
                     /locus_tag="VGP27_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEV7852961.1"
                     /translation="ASPSGARTERGFSMSLDGTLEGRYPGVALIIARDKAGRVQGFHR
                     YATAGGGAEVTLDVPWRRREAPNGIDERLSVDMIANARQQGAQRLSLAFAAFPEIFTD
                     TDRDLPSRLVYTLVHLGDPLIALESLYRYLRKFHALGDRRYVMATLTQVIPLLIVLLS
                     LEFVPRRRHLTRASVQQAS"
     gene            complement(513..1040)
                     /locus_tag="VGP27_16645"
     CDS             complement(513..1040)
                     /locus_tag="VGP27_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEV7852962.1"
                     /translation="MSEPQPPQYPQYPQYPQYPPPPSPGGGYPGGYPPPPPQPYAGYA
                     PPPAAPKNGLGVAALVVAIVALISVVGGVVLGIVAIILGVLGWQRARRGEATNGGIAI
                     AGIVLGILSIIEALVVIALSVWVFNEVGGTDYMDCLSRAGSDQQAVQRCVDQFQNRVE
                     DQFSVTITTPAAPTP"
     gene            complement(1037..1627)
                     /locus_tag="VGP27_16650"
     CDS             complement(1037..1627)
                     /locus_tag="VGP27_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV7852963.1"
                     /translation="MLPHLWKSALLSGLSAVILGVLVLAWPGKSILVAAILFGAYLLV
                     TGISQVIMAFALHVTASGRVLLFISGAAALILAVLAFRHFGEGYAVLLLAIWIGVGFI
                     FRGVATTVAAVSDPALPARGWNIFIGVVSLIAGVVVIASPFESIVTLALVVGVWLIVI
                     GVFEVVSAFGIRKAAKSVSARTAPAEPTAPAEPSAS"
     gene            1791..2735
                     /locus_tag="VGP27_16655"
     CDS             1791..2735
                     /locus_tag="VGP27_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7852964.1"
                     /translation="MQKVEATVLGGREIRRGTMCRIAAVIAASATLAVSGCSAGGDSK
                     SSESTSATSAVSAEKVDSIANTVPEDIKSSGKLIVGVNIPYTPNEFKDPSGKIVGFDV
                     DLMNAVAGTLGLTPEYREADFAKIIPSIQGNTFNVGMSSFTDTKEREQTVDFVTYGSA
                     GILWAQRPDNLIDPNNACGKKVAVQATTTEETDELPAKSKACTDAGKPAIQIVSFDGQ
                     DAATNAVILGQADAMSADSPVTLYAIKQSNGKLTQAGEITDAAPYGWPVKKGSPLAQS
                     LKQALEHLIESGKYKEIATNWGVEKVMIDKPVINGAIS"
     gene            2749..>2852
                     /locus_tag="VGP27_16660"
     CDS             2749..>2852
                     /locus_tag="VGP27_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7852965.1"
                     /translation="MTDVDTPPTTAPAAIDAVPLRHPWRWVAAVVIVIL"
BASE COUNT          571 a          907 c          927 g          447 t
ORIGIN      
        1 cgcgtcgccg agcggtgccc gcacggagcg cgggttctcg atgtcgctcg acgggacact
       61 cgagggcagg taccccggtg tcgcgctgat catcgcgcgg gacaaagccg gccgcgtgca
      121 ggggttccac cgctacgcca ccgcgggcgg cggcgccgag gtgacccttg acgtgccgtg
      181 gcgacgccgt gaagccccca acggaatcga cgagcggctg tcggtcgaca tgattgcgaa
      241 cgccagacag caaggggcgc aacggttgtc gctcgccttc gccgcgttcc cggaaatctt
      301 caccgacaca gaccgtgatc tgcccagcag gctcgtctac accctggtcc atctgggcga
      361 cccgctgatc gcgctggagt cgctgtaccg ctatctacga aagtttcacg cgctcggcga
      421 ccggcggtac gtgatggcga cgctcaccca ggtgattccg ctgcttatcg tgttgctgtc
      481 gctcgagttc gttccgaggc gacggcacct gactagggcg tcggtgcagc aggcgtcgtg
      541 atcgtgacgc tgaactggtc ttcgacgcgg ttctggaact ggtcgacgca ccgctgaacg
      601 gcttgctggt ccgaaccggc cctcgagagg cagtccatgt agtcggtacc accgacttcg
      661 ttgaaaaccc acaccgaaag cgcaatgacg acgagtgcct cgatgatgct gagaatgccg
      721 agcacgatgc ctgcgatcgc gatgccgccg ttggtggctt cgccgcgtct ggcgcgctgc
      781 cagcccagga ccccgaggat gatcgcgacg atgccgagca ccactccgcc gacgacggaa
      841 atgagcgcga cgatcgcaac aaccagcgcg gcgaccccga gcccgttctt cggcgcggcg
      901 ggcggcgggg cgtatcccgc atacggctgg ggcggcggcg gcggataccc gcccggatac
      961 ccgccaccgg ggctcggcgg cggcgggtac tgcggatatt gagggtattg agggtattgc
     1021 ggcggctgcg gctcgctcat gatgccgatg gctcggcggg cgccgtcggt tctgccggtg
     1081 cggtgcgtgc agagaccgac ttggcggcct tgcggatgcc gaacgccgac acgacctcga
     1141 atactccgat cacgatcagc cagacgccga cgacgagggc cagggtgacg atcgattcga
     1201 acggtgaggc gatcaccacg acgccggcga tgaggctgac gacgccgatg aagatgttcc
     1261 accctctggc gggcagggcg ggatcgctca cggcggccac cgtcgtcgcg acaccacgaa
     1321 agatgaaccc gacgccgatc cagatcgcca gcagcaaaac cgcgtagccc tcgccgaaat
     1381 ggcgaaatgc gagcacggcg aggatcagcg ccgcggcacc gctgatgaac agcagcaccc
     1441 ggccactggc cgtcacatgc agggcgaacg ccatgatgac ttgcgagatg cccgtcacca
     1501 gcagatacgc accgaacaag atggcggcga ccagtatcga cttcccgggc cacgcaagca
     1561 cgaggacacc gaggatcacc gccgagagcc cggacaacaa cgccgacttc cagaggtgtg
     1621 gcaacagggc cggaggggct tcggtagtca tgaccgaagt gtttcacagc gcttaccgcg
     1681 cagataagag tttgcacgca acgcgcggga aattcggccc gacagcacaa taagcggaag
     1741 cgcggcgcaa cgccgtcgtc gccgttaacc ttccgtacgt taactgccca atgcagaaag
     1801 tagaggcaac cgtgttaggt ggacgtgaaa tccgtcgggg gacgatgtgt cgcatcgcag
     1861 ccgtaattgc tgccagcgcc accctcgccg tttcaggctg ttcggctggc ggcgattcca
     1921 agtcgagcga gtcgacgagc gcaacctcgg cggtgagcgc cgagaaagtt gattcgatcg
     1981 ccaacaccgt gcccgaggac atcaagtcgt ccgggaaact gatcgtcggt gtcaacattc
     2041 cgtatacgcc aaacgagttc aaggatccca gcggcaagat cgtcggcttc gacgtcgatc
     2101 tgatgaacgc ggtcgccgga acgctcggcc tgacaccgga ataccgcgag gccgacttcg
     2161 ccaagatcat cccgtcgatc cagggcaaca ccttcaatgt cggcatgtcg tcgttcaccg
     2221 acaccaagga gcgtgagcag accgtcgatt tcgtcaccta cggctcggcg ggaatcctgt
     2281 gggcgcagcg tcccgacaac ctcatcgacc cgaacaacgc ctgtggcaag aaggtggccg
     2341 tgcaggcgac aacgaccgag gagaccgacg agctgcctgc caagagcaag gcgtgcaccg
     2401 acgcgggtaa gcctgcgatt cagatcgttt cgttcgacgg ccaggacgcg gcgaccaacg
     2461 ccgtgatact tggtcaggcc gacgcgatgt cggcggactc cccggtgacc ctgtacgcga
     2521 tcaagcagag caacggcaag ctgacacagg ccggtgagat cactgatgcc gcgccgtacg
     2581 gttggccggt gaagaagggc tccccccttg ctcagtcgct gaagcaggct ctcgagcatc
     2641 tgatcgagtc cggcaagtac aaggagatcg cgaccaactg gggcgtcgag aaagtgatga
     2701 tcgacaagcc ggtgatcaac ggcgcgatca gctagttggg atcctccaat gactgatgtc
     2761 gacacgccgc caaccaccgc cccagctgcg atcgatgctg ttccactgcg tcatccgtgg
     2821 cggtgggtgg cggcggtcgt catcgtcatc ct
//
LOCUS       DASYLX010000708         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3440958,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000708 DASYLX010000000
VERSION     DASYLX010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440958"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..257
                     /locus_tag="VGP27_16665"
     tRNA            185..257
                     /locus_tag="VGP27_16665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Lys,seq:ttt)
     gene            560..835
                     /locus_tag="VGP27_16670"
     CDS             560..835
                     /locus_tag="VGP27_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV7852966.1"
                     /translation="MTRHRLSRSGDRAAKNALYRIALVRMSADPQTRDYVERQIGRVA
                     PRRFCGYSNEPSPAKYSDCCGCRLRSASSARLRRCSALVGRHFYANR"
     gene            876..>1688
                     /locus_tag="VGP27_16675"
     CDS             876..>1688
                     /locus_tag="VGP27_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEV7852967.1"
                     /translation="MTARPWSMAARSAFIAGAVVFIALAAAGAALTVALYHALLSGVD
                     DAAGRRVGDIVAGMKVDATADLDAPLLDTDQRIVAVQVLDGHGNIIAHSDGAPASPLV
                     APSRVGPTLREGLTDNAPPDNDERVSGSTVDTDVGRYTVLVAGDIESAEATVRTALFL
                     LAGACPFVVAVAAIATYRLMRRSLRSVDAIRARVAEISTSDLTERVPVPPTRDEISAL
                     ATTMNDMLARIESGHAAQRQFVGDASHELRSPLTAIISALDVAVVHPEIFDLQ"
BASE COUNT          289 a          549 c          544 g          306 t
ORIGIN      
        1 cgcgctaccg ccttgaaagg gcggcgtcct aggccgctag acgaaggggg ccagccgaat
       61 ctctccgggg tactcgcaac gccgtgacgc tgggagcctc gatagcttag ggtaccggga
      121 ccccaatgct caaacgagat gtccggcccg aggcagtatc ctttctgggg tccagattca
      181 caatgcccct atagctcagt tggtagagct acggactttt aatccgcagg tcctaggttc
      241 gagtcctagt gggggcacag atcgcaacgt tcgaccacgc ggcaaacgtc ggccgagggc
      301 ggggcaggca gtcaccgacc ccggtgtcgg cggcgacggc gacggcgacg gcgatcatgg
      361 ctggccgcag cgctccttcc acgaagttat gtactccggg cagcaggatg gcggctccta
      421 agtcagcgcc cagaatcccg tactgtacga gcctcgaccc ggtcgccccg caccgccggc
      481 actaacccgc accctgctgc gcaatgacgc ccccttcgcc gcgctgtgcg gggcggcccg
      541 gcgccggcgt cctcgcagaa tcacccgtca ccgcctgtcc cgcagtggcg atcgagcagc
      601 caagaatgcc ctgtaccgca tcgcgctggt gcgaatgtcg gccgatccgc agacccgcga
      661 ctacgtcgag cgccagatcg gaagggtcgc accaaggaga ttctgcggct actcaaacga
      721 gccatcgccc gcgaagtatt cagactgctg cggctgccgt ctacgctcag cgtcgtcggc
      781 gaggttgcgc cggtgttccg ctctcgttgg aaggcatttc tacgcgaatc ggtagcgacg
      841 ggtctcaggc cgccaagcgg cgcggggttt cacgggtgac cgcacggccg tggagcatgg
      901 cggcacgttc ggctttcatc gccggcgcgg tcgtgttcat cgcgctggcg gcagccggtg
      961 ccgccttgac cgttgcgctc tatcacgccc tcttatcggg cgtggatgac gccgctgggc
     1021 gtcgcgtcgg cgacattgtc gcgggcatga aagtcgacgc caccgcggac ttggacgccc
     1081 cgctgctaga cactgaccaa cggatcgtcg ccgtgcaggt actcgacggc cacggaaaca
     1141 tcatcgcgca ctccgatggt gcgccggctt ctccgttggt ggctcccagc cgggtgggac
     1201 cgaccctgcg cgaggggctc actgataacg ccccaccaga caatgatgaa cgcgtcagcg
     1261 gaagcacggt ggataccgac gttggccggt acacggttct tgtcgctggg gatattgaga
     1321 gcgcagaggc tacagtgcgg actgcgcttt tcttgctggc gggcgcgtgt ccgttcgtcg
     1381 tcgcggtcgc cgccattgcc acctatcggc tgatgcgacg ctctttgcgc tctgttgacg
     1441 caatccgggc tcgagtcgct gagatcagca catctgattt aacggagcga gtgccggttc
     1501 caccgactcg cgacgaaatc tcggcgttgg ccaccacgat gaacgatatg ttggctcgta
     1561 tagaaagcgg ccacgcggca cagcgccagt tcgtcggaga tgcgtcccat gagctacgaa
     1621 gcccgttgac ggcgatcatc tcggcacttg acgtcgcggt ggttcacccg gagatcttcg
     1681 atctccag
//
LOCUS       DASYLX010000709         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_148920,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000709 DASYLX010000000
VERSION     DASYLX010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148920"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..421
                     /locus_tag="VGP27_16680"
     CDS             26..421
                     /locus_tag="VGP27_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7852968.1"
                     /translation="MTVRRGLYGMFAGGLLGGVASAAMIMPSANAEPVTPVTPDTCTF
                     SNAANTSSTVSAQMSTYLTAHPQANKDLTEIAKQPMDEAQIAYRAYFANNPQVETELK
                     AINQPAADLVSQCGIVVRPTPVSEALQGV"
     gene            complement(521..1435)
                     /locus_tag="VGP27_16685"
     CDS             complement(521..1435)
                     /locus_tag="VGP27_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7852969.1"
                     /translation="MSREESSGRTSPPTERVVAVLEFLGRHPHDRFGVSELSRRLNLS
                     KPTCLGILTTLTDCDYLMRDFRDKSYRLGPALISLGHTAQEALRVDPAARAELRRLSA
                     ISNTTAALSAVIDDRITVLELVGPNGADVGVRVGQSYPFAPPVGLMFVLWDDAALRGW
                     LAKEPTIPLRTDGERLDRVIDECRRTGYLVERLTPGGRRLYALMAGVSSRLPDELRAL
                     LGELIADIGERVYLRSEIPAGSRQRHDISVISAPVFDHHLHQAMVVSLQIGRALTDAD
                     IAKSARALTATADVLTSRLGGMKPAFDR"
     gene            1525..2652
                     /locus_tag="VGP27_16690"
     CDS             1525..2652
                     /locus_tag="VGP27_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852970.1"
                     /translation="MYSQPLTDAIAEAERLVVDAPFIESEADLLEGLQYLAGCIAGCT
                     HLAFDYDRDHPFMHSGTGPFTKMGLDNPDTLYFGTRVQPGHEYVVTGKRGTTTDLSFQ
                     MLGGEYTDDNVPVSQAAFDDRELDIASDGTFQWRVTPNSPSQLLIREVYNDWSAQRGY
                     VSIARTDNAGTAPPPLTRALIEKRYAVAGKQLVQRVKTWLQFPQWFYNTLPVNTMVAP
                     RLTPGGLATQYSSVGHFDMDPEQALVITLPVTDAPYLGFQLGSLWYISLDYINHQTSL
                     NGTQAQADPDGKIRFVVSDANPGVTNWVETLGHRKGYLQFRWQRLSRELTEADGPTVE
                     LLDIDKVAAALPYYESNKISDEAWRARIALRQKQIGDRMVA"
     gene            2654..3439
                     /locus_tag="VGP27_16695"
     CDS             2654..3439
                     /locus_tag="VGP27_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7852971.1"
                     /translation="MVAELLKDRVVVISGVGPALGTTLARRCAEAGADLVLAARTVER
                     LDDVAKQITDLGRRAVSVGTDITDDAQVANLVSATLEAYGRVDVLINNAFRVPSMRPF
                     ANTTFDHMRDAIELTVFGALRMVQGFTPALAESKGSVVNVNSMVVRHSQAKYGAYKMA
                     KSALLAMSQTLATELGEQGIRVNSILPGYIWGGTLEGYFKHQAGKYGTTVEEIYNAAA
                     VNSDLKRLPTEDEVASAILFMASDLSSGITGQALDVNCGEFKA"
     gene            3525..4670
                     /locus_tag="VGP27_16700"
     CDS             3525..4670
                     /locus_tag="VGP27_16700"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7852972.1"
                     /translation="MSPRTDPGSVDDLLTSATKACGLDDFGSDDDNYREALGVLLESY
                     RRDADLTEAGSKMNRFFVRNALVARLMSEAAWKQHPQHVDVAIERPIFVTGLPRTGTT
                     ALHRLMSADPRHQGLELWLAEFPQPRPPRETWAENPVFRELDARFTQAHDENPDYTGL
                     HFMTADEVEECWQLLRQSLHSVSYETLAHIPTYSQWLAKQDWTRPYQRHRKNLQLIGV
                     NDAEKRWVLKNPSHLFALDALMAAYPDALVVQCHRPVETIMASMCSLAQHTTEGWSKS
                     FTGDLIGRDAMETWSRGLELFTAERANHDSAQFYDMDYFELIKDPIGTVEQIYRHFGI
                     EMTDDARAAMQASDEASKQGPRAPKHTYSLEDYGLTADEVKARFKGL"
     gene            complement(4999..>5372)
                     /locus_tag="VGP27_16705"
     CDS             complement(4999..>5372)
                     /locus_tag="VGP27_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV7852973.1"
                     /translation="SPACRADARSIPAGHRPSGRLPMRVESEYRRHGTLGYLAAYDVH
                     RAQVIGHCAPSTGIEPFTALVDKVMTRAPYASAQRVFWVVDNGASRRGWTAAARLRDA
                     FPIARMIHLPVHASWLNQIKI"
BASE COUNT         1025 a         1708 c         1741 g          898 t
ORIGIN      
        1 gtgttttctg atgaagatgt ctgaaatgac cgttcgacgc ggcctgtacg gcatgttcgc
       61 aggcggcctg cttggtggcg tcgcatccgc ggcgatgatc atgccgtccg ccaatgctga
      121 gcctgtgaca cccgtgacgc cggatacgtg cacgttcagc aacgctgcca acacgtccag
      181 cacggtatcg gcccagatga gcacgtatct cacggctcat ccgcaggcca acaaggacct
      241 gacggagatc gcgaagcagc cgatggatga ggcgcagatc gcctaccgcg cgtacttcgc
      301 gaacaacccg caggtcgaaa ccgaattgaa ggccatcaac cagcctgctg ccgacctggt
      361 cagccagtgc ggcatcgtgg tccggccgac accggtctcg gaggccctgc agggcgtcta
      421 accgtttggc aatgacacca ccgctcatcg cgtcggcgct cactgccgac gcgatgagtg
      481 cgtgttcggg acaccgcgcc tgcctcgggc agatgaccct ctagcgatcg aatgccggct
      541 tcattccgcc cagccggctg gtgagcacat cggccgtcgc cgtcagcgcg cgggcgctct
      601 tggcgatgtc ggcatcggtc agggcccggc cgatctgcag cgacacaacc atcgcctggt
      661 gcagatggtg gtcgaacacc ggcgcggaga tgacgctgat gtcgtggcgc tgccggctgc
      721 ccgccgggat ctcggaacgc aggtagaccc gctcgccgat gtcggcgatc aactcgccga
      781 gcagcgcgcg cagttcgtcg ggcagcctgc tcgatacccc ggccatcagc gcatacagcc
      841 gccgcccgcc gggagtgagt cgctcgacga ggtatcccgt gcgacggcat tcgtcgatga
      901 cgcggtccag tcgctcaccg tcggtgcgga gcggaatggt gggctccttg gccagccaac
      961 ccctcagggc ggcgtcgtcc cacagcacga acatcagccc gaccggaggg gcgaacggat
     1021 agctctgacc gacccgcaca ccgacgtcgg cgccgttcgg cccgacgagt tccagcacgg
     1081 tgatgcggtc atcgatcacc gcggacagcg cagcggtggt gtttgagatc gccgacagcc
     1141 tccgcaactc cgcgcgcgcg gccggatcca cccgcaacgc ctcctgggcg gtgtgcccca
     1201 acgagatcag cgcagggcca agccggtagg acttgtcgcg aaagtcacgc atcaggtaat
     1261 cgcagtcggt cagcgtggtg aggatgccca ggcaggtcgg cttgctcagg ttaagccgac
     1321 gagacagctc ggaaacaccg aatcggtcgt gcggatggcg tccgaggaat tcgaggacgg
     1381 cgaccacccg ctcagtgggc ggcgacgtcc gaccggacga ttcctcgcgg gacatatcaa
     1441 aaatgtacct ttgcgttcca aatatcgaac aataggtcgg tgccgaccgt tgacgagaat
     1501 gagaacacgt tctagtttct aggggtgtac tcccagccat tgaccgacgc gatcgcggag
     1561 gccgagcgat tggtcgtcga cgcacccttc atcgaatccg aggccgatct gctcgagggc
     1621 ctgcagtatc tggccggatg tatcgccgga tgcacgcacc tggccttcga ttacgaccgc
     1681 gaccatccgt tcatgcactc ggggaccggc ccgttcacca agatgggttt ggacaacccc
     1741 gacacgctct acttcggcac gcgggtgcag cccggtcacg agtacgtcgt caccggcaag
     1801 cgcggcacca ccaccgacct gagctttcag atgctcggcg gcgagtacac cgatgacaac
     1861 gtgcccgtca gccaggcggc gttcgacgac cgcgaactcg acatcgcgtc cgacggcacc
     1921 ttccaatggc gagtcacgcc gaacagcccg tcgcagttgc tgatccgcga ggtgtacaac
     1981 gactggtccg cccagcgcgg atacgtcagc atcgccagga ccgacaacgc gggcaccgcg
     2041 ccgccgccct tgaccagagc gctgatcgag aagcgatacg cggtggcggg aaagcagcta
     2101 gtgcagcggg tgaagacctg gctgcagttt cctcagtggt tctacaacac gctgccggtg
     2161 aacacgatgg ttgcgcctcg gctcaccccg ggcggcctgg ccacccagta ctcctcggtc
     2221 ggtcacttcg acatggaccc cgagcaggcg ttggtcatca cactgccggt caccgatgcg
     2281 ccgtacctcg gattccagct cggcagcctc tggtacatct cgttggacta catcaaccac
     2341 cagacatcgt tgaacggcac tcaggcgcaa gcggatcccg acggcaagat ccgtttcgtg
     2401 gtgtcggatg cgaaccccgg cgtgacgaac tgggtggaga cgctcgggca ccgcaagggc
     2461 tatctgcagt tccggtggca gcgattatcg cgtgagctca ccgaggctga cggtcccacc
     2521 gtcgaactgc tcgacatcga caaggtggcg gccgcgctgc cgtactacga gtcgaacaaa
     2581 atctccgacg aggcgtggcg agcgcggatc gcgctgcggc agaagcaaat tggcgacagg
     2641 atggttgcgt aagatggtag cggaactcct caaggaccga gtcgtggtca tcagcggtgt
     2701 cggtccggcg ctgggcacca cgctggcgcg gcgatgcgcg gaggcaggcg cggacctggt
     2761 gctggcagcc agaaccgtcg agcgcctcga cgatgtcgcc aagcagatca ccgatctcgg
     2821 caggcgtgcg gtgtcggtgg gcaccgacat cacagacgac gcgcaggtgg cgaacctcgt
     2881 ctcagccacc ctcgaggcgt acggcagggt tgatgtgctg atcaacaacg cgtttcgggt
     2941 gccgtcgatg aggcccttcg ccaacaccac gttcgaccac atgcgcgacg ccatcgagct
     3001 gaccgtcttc ggtgccttgc gcatggtcca gggcttcact cctgcgcttg ccgagtccaa
     3061 gggttcggtg gtcaacgtga attcgatggt ggtgcgccat tcgcaggcca agtacggcgc
     3121 ctacaagatg gccaagtcgg cgttgctggc catgtctcag acgctggcca ctgaactcgg
     3181 cgaacagggc atccgggtga attcgattct gcccggctat atctggggtg gcacgctgga
     3241 gggctacttc aagcaccagg ccggcaagta cggcaccacg gtcgaagaga tctacaacgc
     3301 ggccgcagtc aacagcgacc tcaaacggct gcccaccgag gacgaagtgg cctccgcgat
     3361 cctgttcatg gccagcgatc tgtccagtgg catcaccggc caggcgttgg acgtgaactg
     3421 cggagagttc aaggcatgag tccagcaatg agccggcgca gacgagcgcg agtgaggatc
     3481 gcagcgaggg acgagcgagg accggaacga gcgggagtcc agcaatgagc ccgcgcaccg
     3541 acccaggttc cgtcgacgac ctgctcactt cggcgaccaa ggcttgcggg ctcgacgact
     3601 tcggctccga cgacgacaac tatcgggaag ccctcggcgt gctgctggaa tcctatcgac
     3661 gtgacgccga cctgacggag gccggcagca agatgaaccg cttcttcgtc cgcaacgcgc
     3721 tggtcgcgcg gctgatgtcc gaggcggcct ggaagcagca cccccaacat gtggatgtgg
     3781 cgatcgaacg gccgatcttc gtcaccgggc tgccgcgcac cggaaccacg gctctacacc
     3841 ggctgatgag cgccgacccc cgacatcagg gtctcgagtt gtggctcgcc gagtttccgc
     3901 agccgcgccc gccgcgcgaa acctgggcgg agaaccctgt tttccgtgaa ctcgatgccc
     3961 gattcaccca agcgcacgac gagaatcccg actacaccgg gctgcatttc atgacagccg
     4021 acgaggtcga agagtgctgg cagctgctgc ggcagtcgct gcactccgtg tcctacgaga
     4081 cgctcgcgca tattccgacg tattcgcagt ggctggccaa gcaggattgg accaggccct
     4141 atcagcgaca ccgcaagaac cttcagctga tcggagtgaa cgacgcggag aagcgctggg
     4201 tgctcaagaa ccccagtcac ctgttcgcgc tcgacgcatt gatggccgcc tatcctgatg
     4261 cgctcgtcgt gcaatgccac cgacccgtcg agaccatcat ggcgtcgatg tgctccctgg
     4321 cccagcacac cactgaaggc tggtcgaaaa gcttcaccgg agacctgatc ggccgggatg
     4381 cgatggagac atggtcgcgg ggcctcgaat tgttcactgc cgaaagggca aatcacgatt
     4441 cggcccagtt ctatgacatg gactacttcg agctgatcaa ggatccgatc ggtaccgtcg
     4501 agcagatcta ccggcatttc ggcatcgaga tgaccgacga cgcgcgcgcc gcgatgcagg
     4561 ccagcgacga ggcgagcaag cagggaccgc gtgcgcccaa gcacacctac tcactcgagg
     4621 attacgggct gaccgccgac gaggtcaagg cccggttcaa agggctgtga cttctctgct
     4681 cgcgaggaaa actcagccgt cgcgtcttca gcaagtgccg atttccgcat gggggtcgtg
     4741 gctcatcggg ggaagatcac agccctcacg cagaattcgt tgattcgcga catgtgacta
     4801 gtggtctcgc cgttagttcg tcgcgggtca tgcggggggc gcgggtcagg aggcggttga
     4861 ggtcgtcgcg gttgcagcgc cagtcgaaag gtcgggccgc ggtgttgtag cgcgcttcga
     4921 acgcggtgag gcgattagcg aggacgtcaa ggtcggcaac gtcgtcgggc gtcaacagtt
     4981 tcgctgcaca acggcgaact agattttgat ctggttgagc caagatgcgt gcactggcag
     5041 gtggatcatc ctggcgatgg gaaacgcatc ccgcagccgt gcggcggcgg tccagccgcg
     5101 ccgtgaggcg ccgttgtcga cgacccagaa cacccgctgt gccgaggcat agggtgcgcg
     5161 ggtcatgacc ttgtcgacga gcgcggtgaa cggttcgatc ccggtactgg gtgcgcaatg
     5221 gccgatcacc tgagcgcggt gcacgtcgta ggcggccagg tagcccagcg tcccgtggcg
     5281 gcggtactca ctttcgaccc gcatgggcag tcggcccgac ggacggtggc cggctgggat
     5341 ggatctggca tcggccctgc acgccgggct tc
//
LOCUS       DASYLX010000710         7716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2343016,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000710 DASYLX010000000
VERSION     DASYLX010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343016"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VGP27_16710"
     CDS             <1..120
                     /locus_tag="VGP27_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEV7852974.1"
                     /translation="HGISNLRVADASVMPSPISANTNATVYAIAERAAELIRS"
     gene            150..692
                     /locus_tag="VGP27_16715"
     CDS             150..692
                     /locus_tag="VGP27_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852975.1"
                     /translation="MAESPIAVFDKPTRTDLPERTPVLFEAACVLGGSIAGLLAARVL
                     ADHARTVLIIERDQVNMEGRPRAGVPQGRQGHGLLPGGLAQIERWLPGFTRGARDLGG
                     VLVGPGQQVVYLDGHQQVRSGDAQILIGSRPFLESRIRSRVLALPNVSTLSAQATGLG
                     YRNDAVSAVRCGTWRMASRT"
     gene            1035..1442
                     /locus_tag="VGP27_16720"
     CDS             1035..1442
                     /locus_tag="VGP27_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852976.1"
                     /translation="MRRPVHERVVGVEGPLVGGFVMGNLARLAEPGPDCGAARGPDAV
                     DGSGALGVGESGGPGWCSRQPGQTTSSAVAGTAKVNLQRAQARSASRRTIAAAVVLKS
                     AAHTAHVTSTTWSGLMPPSSAGAYARPSGTAST"
     gene            complement(1384..1920)
                     /locus_tag="VGP27_16725"
     CDS             complement(1384..1920)
                     /locus_tag="VGP27_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852977.1"
                     /translation="MMRKLLEERIAQWKIHDPYLHTRVAAETYETATSVLRIRNEAHR
                     AARDSRVVVVPSDVALIYKPGKDFGGRTADKFKWVPIYHTDDRGEPHLLTMEVCVTRK
                     REKTTPSPLSTIIETLQDAPDLVSLGRTRMGSANRAPYAIGDDGHPTPRPSACSVRVV
                     KCWPFRSAWRTRPPTTAA"
     gene            complement(2243..3898)
                     /locus_tag="VGP27_16730"
     CDS             complement(2243..3898)
                     /locus_tag="VGP27_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852978.1"
                     /translation="MSAHAVRVGSIATPAHGSALAGTAYILRFMLRRDWVRLAVWSGS
                     LVAFCVYYVVAIAAVYPTAADRQNRAATMSNPGGVLLGGPGYGLEHYTIGAMFANEMT
                     LWLMVFLATFNILQITRNTRTEEATGRTELVRALPIGRHAASVAAFLVVVIADAVFAL
                     LGSVLLIGIGHLAVPDTVAMMAGLAMTALVFAGVTTVTCQLTAHGRGASGLAFAVLGA
                     TVLTRGIGDIQKLHGGWLSWLSPIAWTQQMRGYVDLRLWPLGLSVMAIIVALALGAFL
                     ASRRDLGGGLLRERTGRADAMPALASPFALALRQQRSTLLWWLVGCVAMFGLSGLFLG
                     KGAVDALRSIADQNSLTSTIFGDDPLSAFLAIMMLHNALAVAVFAIAVVLRVKPEEDE
                     GRFGLGLSRSVSRTSLLLSHLTVVALGASVLLFVGGALALWTGGLLSGGNVGIEVLLK
                     SAGAFAVGIAVLIAFTAALYAWIPKASPLAWVLFAVVVVQSFFGSLLKLPDAVSALSP
                     FWWIGDYPRSPLEQSHMIGLGTAAVVLLALAVTGFRRRDLTAG"
     gene            complement(3895..4854)
                     /locus_tag="VGP27_16735"
     CDS             complement(3895..4854)
                     /locus_tag="VGP27_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7852979.1"
                     /translation="MSENPIEIIGLHKSFGSVKALDGLHLTVRPGEVAGFLGPNGAGK
                     STTIRVLLGLQRSDSGTARIFGGDVWRDAVALHRRLTYVPGDVSLWPNLTGGEAIDYL
                     TRLHRRSSDNARQKARKAELLERFELDPTKKARTYSKGNRQKVALVAAFLVEPELYIF
                     DEPTSGLDPLMEAVFTEHVQEVKAAGKAVLLSSHILSEVEKLCDTVTIIRSGRTVESG
                     TIAELRHLTRTTISVLITQHPAALATLTGVHDLVASTSRNGTQVEFSVDNAKMGDVLI
                     ALTRLGIRGLTATPPSLEELFMRHYGGVSDTANSHRGSEVVHR"
     gene            complement(4984..5796)
                     /locus_tag="VGP27_16740"
     CDS             complement(4984..5796)
                     /locus_tag="VGP27_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7852980.1"
                     /translation="MSTATHTALARPQWFSDTLTMFRRALLHNVRNPTGPISAIAGGI
                     VILLLSVYVFGGTLGAGLVPGGTREDYLDYIAPAIVLMGAIGIMQMIAVWVATDMSEG
                     IIARFRTMAIARSSVLAGHAYSGVVLVIITIAPLLGFAVLLGYRPHADASQWLAVVGF
                     ALLLGIAVSWLSVAFGLVPRRVENASTLPLIVLILPMVSSGFVPVASFPAWVKGFAQY
                     QPFTPIINTLRGLLAGTPRASDVAWAIGWTIAIAIVGYVWSLWLYRRRAAGR"
     gene            complement(5793..6746)
                     /locus_tag="VGP27_16745"
     CDS             complement(5793..6746)
                     /locus_tag="VGP27_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7852981.1"
                     /translation="MSAPTQPAIQIRGLQKSFKDNRVLRGVDLDIPAGIVFALLGANG
                     AGKTTTINILTTLLAADAGTVRVAGFDVVSHAEQVRASIGVTGQFAAVDGLLTGQENL
                     RLMARLAHLGSETAHNRIAELLDQFGLTEAARKPASTYSGGMRRRLDLAMSLVLDPAI
                     IFLDEPTTGVDPRGRAVMWDIVRGLAAQGKTIFLTTQYLEEADQLASRVAVIDSGTII
                     AEGTPAELKRRVGTGHLVLDLPTPNALATASRVLPGATADMDALALRVDGDTDADSIR
                     DTLVRLDAAQVPISSLRIVTPDLDDVFFALTGTAKTKKNHE"
     gene            complement(6950..7444)
                     /locus_tag="VGP27_16750"
     CDS             complement(6950..7444)
                     /locus_tag="VGP27_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7852982.1"
                     /translation="MLTDEQLRFCDDLSRFFVREMAFPPVAGRVLAYLAVCNPAAQTI
                     NDLADALLASRSAITQAVVLLEGRNMARRTRARGERVDRVAAIFDVSLFERDFDAAGY
                     TQQAALIRRGVALLAEDDDSGRRQGLEQLAALNDFLAEKYPLLKEEWLARLRGTDAEE
                     MPKR"
BASE COUNT         1355 a         2389 c         2709 g         1263 t
ORIGIN      
        1 cacggcatca gcaacctgag agtcgccgat gcatcggtga tgccctcacc gatctccgcc
       61 aacaccaacg cgaccgtcta cgcgatcgcc gaacgagcag cagaactcat ccgttcgtga
      121 ccaatgatgt cagcaacgca gaagcagtcg tggctgagtc gccgattgca gtgttcgaca
      181 aaccaactcg gacggatctt cccgagcgca cgccggttct gttcgaagcg gcttgcgtgt
      241 tgggcggcag tattgccggc ctgttggccg cgcgggtcct cgccgaccac gcccgcacgg
      301 tcctgatcat cgagcgcgac caggtcaata tggagggacg cccgcgtgcg ggtgtccccc
      361 agggtcgtca ggggcatggg ctgctgccgg gcgggctcgc gcagatcgag cgttggctgc
      421 cgggcttcac acgtggggcc cgagacctgg gcggggtcct tgtgggtcct ggccagcaag
      481 ttgtgtacct ggacggccac cagcaggtgc gtagcggcga cgcgcagatt ctcatcggca
      541 gtcgaccctt cctggagtcc cggattcgta gccgcgtcct tgctctgccc aatgtgtcga
      601 cgttgtcggc acaggcaacc ggcctcggat atcgcaacga tgctgtgagt gcggtgcggt
      661 gcggtacgtg gcggatggcg tcgaggacgt gatgagcgtc ggcttcgtgg tggacgccat
      721 ggggcgggcg agcaggttgt cagaatgggt cgagcaggcc ggctacgacc ggccgcggct
      781 gcagcggctg cggaccggca tcgctcggcg gcccgctcct ccagcagccg ctcgatggcg
      841 gactggagga tcatcagcgg gtaagggtgc ctggactcga cctcgtcgaa ttccagatag
      901 aggccggaga agtggcccac cggctgtagt tctccctccg cgaggaagcg atccaggatt
      961 cccgagcaga ccccgaactc cagacgccgg ccggcgcggg cggatgtctc gcagtcgtgg
     1021 tggaacctga gctggtgcgc cgccctgtcc acgagcgggt cgtcggtgta gaagggccgc
     1081 tcgtcggcgg gttcgtcatg gggaacctgg cgcggctcgc ggagccgggc ccggactgcg
     1141 gcgcggcccg cggtccggat gcggtcgacg gcagcggcgc gctgggggtc ggcgagtctg
     1201 gggggccggg ctggtgcagt cggcagccgg ggcagaccac gtcatcggcg gtggccggca
     1261 cggcgaaggt caacttgcag cgggcgcagg cacggtcggc gtcgcgaagg acgatcgccg
     1321 cggcggtcgt gttgaagtcg gcggcgcaca ccgcacacgt cacctcgacg acgtggtcgg
     1381 ggcttatgcc gccgtcgtcg gcgggcgcgt acgccaggcc gagcggaacg gccagcactt
     1441 gacaacacgg acactgcagg cagacggtcg tggtgtcggg tgcccgtcgt cgccgatcgc
     1501 gtagggtgcc cggttcgccg agcccatgcg tgttcttccg aggcttacca gatcaggagc
     1561 atcctgcaac gtctcgatga tcgtgctcaa cggtgaaggt gtggtcttct cacgcttcct
     1621 ggttacgcac acctccatcg tgagcaggtg cggttccccc cggtcgtcgg tgtggtagat
     1681 cggcacccac ttgaacttgt cggcggtgcg gccgccgaag tccttgccgg gtttgtagat
     1741 caatgccacg tcggagggaa cgacgacgac gcgactatcg cgagcagcgc ggtgcgcctc
     1801 gttgcggatc ctcaggacgc tggtggccgt ctcgtacgtc tcggcggcga cccgggtgtg
     1861 caggtacggg tcgtgaattt tccattgcgc gatccgctct tccagcagct tcctcatcac
     1921 gaccccgact gtcacacgga atggtccaac ctcattgcac aaaacagtgg tgcccggcgc
     1981 gcacaaagat caactcagcg cagccatgaa cgaccggcac cgcggcagac caagtcgtcc
     2041 tgcttcgagc caccacgggt tcgaagcagg acgaccaacc agaatgctcc gcgatgtcca
     2101 agggcttcgg ccgagacgtc gccctacgcg gtgaggttcc ttcccgttgc cccctcaccg
     2161 gctccgttcg cggatcggcg atcggcacgc gagacgaaca tgccggtcag ccagccgcac
     2221 agaatcgccg caggacctct actcaacccg cggtgagatc ccggcgacgg aaaccggtca
     2281 cggctagggc caacaagacg actgctgccg taccgagacc gatcatgtgg gattgttcga
     2341 gagggcttct cggatagtcg ccgatccacc agaacggtga gagtgcgctg accgcgtcgg
     2401 gcagtttgag aagtgaaccg aagaagctct gcacgacgac gaccgcgaac agcacccacg
     2461 caagggggct cgccttggga atccacgcat agagcgctgc agtgaaagcg atcagcacgg
     2521 cgatgccgac cgcgaaggct ccagcgctct tcagcaggac ctcgatgccg acgttcccgc
     2581 cggacaagag cccgccggtc cacagcgcca gcgcgccgcc gacgaagagt aggacagacg
     2641 cgccgagcgc gacgaccgtc aagtgggaga gcagcaagct ggtccttgac accgagcgcg
     2701 agaggccgag accgaagcgg ccctcgtcct cctcgggctt cactcgcagg accaccgcga
     2761 tcgcgaatac ggccacggca agcgcgttgt gcagcatcat gatcgccaag aatgcggaca
     2821 gcggatcgtc cccgaagatc gtggacgtga gggagttctg atcggcgatc gatctgaggg
     2881 cgtcaacagc gccctttccg aggaacagtc ctgacaggcc gaacatcgcc acacagccca
     2941 cgagccacca cagcagcgtg cttcgctgct gtcggagggc gagtgcgaac gggctcgcca
     3001 aggccggcat ggcgtcggct cgcccggtcc gttcccgtag caggccaccg ccgaggtcgc
     3061 ggcgggaagc caggaaggct ccgagagcca gcgcgacgat gatcgccatc acgctcagac
     3121 cgagaggcca gaggcggagg tcgacgtacc ccctcatctg ctgggtccag gcgatgggtg
     3181 aaagccatga cagccagccg ccatggagtt tctggatgtc cccgatgcca cgggtcaaga
     3241 ctgtggcgcc gagtacggcg aaggcgaggc cggaggcacc gcgaccgtgc gcggtgagct
     3301 ggcaggtcac cgtcgtgacg ccggcgaaga ccagcgccgt catcgccaga ccagccatca
     3361 tcgcgacggt gtcggggacg gcgaggtgcc cgatgccgat caggagcacg ctgccgagaa
     3421 gggcgaagac cgcgtcggcg atcaccacca cgaggaacgc cgccacgctt gccgcgtgtc
     3481 gtccgatggg cagggcgcgg acgagctcgg tgcgcccggt cgcctcctcg gttcgggtgt
     3541 tgcgggtgat ctgcaggatg ttgaacgtcg ccaggaacac catcaaccac agtgtcatct
     3601 cgttggcgaa catcgcgccg atcgtgtagt gctcaagacc gtagcccggt ccccccagga
     3661 gcactccgcc cgggttcgac atcgtcgcgg ctcggttctg ccggtccgcg gcggtcgggt
     3721 aaaccgccgc gatagcaacc acgtagtaga cgcagaatgc cacgagcgac cccgaccaca
     3781 ccgccagccg gacccagtcg cgccgaagca tgaagcgcag aatgtacgcg gtgcccgcca
     3841 gcgctgagcc gtgcgctggg gtagcgattg agccgacccg tacagcatgt gcgctcatcg
     3901 gtgcaccacc tcagagccac gatggctgtt ggcggtgtcc gacacaccgc cgtagtgacg
     3961 catgaacaac tcctcgagcg acggcggcgt ggccgtgaga ccgcgtatgc ccaacctggt
     4021 cagcgcgatc aagacgtcac ccatcttggc gttatcgacc gagaactcca cctgggtgcc
     4081 gtttcggctg gtggaagcga cgaggtcatg gacgccggtg agtgtggcaa gggcggctgg
     4141 atgctgtgtg atcagtacgc tgatggtggt gcgcgtcagg tgacgcagct cggcgatcgt
     4201 gccggactcg acggtgcggc cgctcctgat gatggtgacg gtgtcgcaca gcttctcgac
     4261 ctcgctcagg atgtgcgagg acagcaacac cgccttcccc gcggccttga cctcttggac
     4321 gtgctcggtg aagaccgcct ccatcagcgg atccaaccct gaggtgggct cgtcgaagat
     4381 gtagagctca ggctcgacaa ggaatgcggc gacgagcgcg accttctgcc ggttcccctt
     4441 cgagtacgtc cgcgccttct ttgtcgggtc gagttcaaag cgctcgagca attcggcctt
     4501 gcgggccttc tgcctcgcgt tgtcggacga cctgcggtgc agccgggtga ggtagtcgat
     4561 cgcctcgccg ccggtgaggt tgggccacag gctcacgtca ccgggaacat aggtaagtct
     4621 gcggtggaga gccacggcgt cgcgccacac gtcgccgccg aagatccggg cggttccgga
     4681 gtcggaacgc tgcagcccga gcagcacccg gatcgtggtc gattttccgg cgccgttggg
     4741 gccgagaaat ccggcgacct ctccgggacg gacggtcaga tgaaggccgt cgagcgcttt
     4801 gacggagccg aaggacttgt gcaggcctat gatttcgatg ggattctcag acatggtcac
     4861 gtccaggtga gacgggatag ttgtcttgtc ctcgcgccaa ggtggagcgc gtcgcccaac
     4921 aaatgacccc ggcgtttccg cgtgacgtgc cacctcaagt gatcttgtga cggtgacggt
     4981 ggttcaccgg ccggccgcgc ggcgtcggta cagccacaga gaccacacat agcccacgat
     5041 ggcgatcgcg atggtccagc cgatcgccca cgcgacatcg ctagcccgcg gggtaccggc
     5101 gagcaggccg cggagggtgt tgatgatggg tgtgaagggt tggtactggg cgaatccctt
     5161 cacccaggcg ggaaaggagg ccaccgggac gaagccgctg ctgaccatgg ggaggataag
     5221 gacgatcagg ggtagggtgc tggcgttctc cacgcggcgg gggacgagtc cgaaggcgac
     5281 cgagagccac gacacggcga tgccgagaag cagggcgaat ccgacgacgg cgagccattg
     5341 cgaggcgtcc gcatgtggac ggtagccgag cagcacggcg aagccgagca gcggggcgat
     5401 ggtgatgatc acgaggacga cgccgctgta ggcgtggccg gcgagcacgg agctgcgggc
     5461 gattgccatg gtgcggaagc gggcgataat gccttcggac atgtcggtgg cgacccacac
     5521 agcgatcatc tgcatgatgc cgatcgcacc catcaggacg atggcgggag cgatgtagtc
     5581 gaggtagtcc tcgcgggtgc cgccaggaac gagtcctgcg ccgagtgtgc cgccgaagac
     5641 gtagaccgac agcaggagga tgacgatgcc tccggcgatt gctgagatcg gtccggtggg
     5701 gttgcggaca ttgtgcagca gtgcccggcg gaacatggtc agcgtgtcac tgaaccactg
     5761 tgggcgtgcg agcgcggtgt gcgtggcggt gctcattcgt ggttcttctt ggtcttggcg
     5821 gtgccggtga gggcgaagaa cacgtcatcg aggtcgggag tgacgattcg caagctgctg
     5881 atgggcacct gtgcggcgtc gagccggacg agtgtgtccc ggatgctgtc ggcatccgtg
     5941 tccccgtcca cgcgcagggc gagggcatcc atgtcggcgg tcgctccggg gagcacccgt
     6001 gacgcggtcg ccagcgcgtt cggggtgggc aggtcgagga cgaggtgccc ggtgccgacg
     6061 cggcgtttga gctccgccgg cgtgccttcg gcgatgatcg tgccgctgtc gatgacggcg
     6121 acgcgggagg cgagctggtc ggcctcttcg aggtactggg tggtcaagaa gattgtcttg
     6181 ccctgcgcgg ccaggccacg cacgatgtcc cacatcacag ctcggccgcg cgggtcgacg
     6241 ccggtggtcg gttcgtcgag gaagatgatc gccgggtcga gcacaagcga catcgccagg
     6301 tcgagtcgac gacgcattcc acccgaatag gtcgacgccg gtttccgcgc cgcctccgtg
     6361 aggccgaact gatcgagcag ctcggcgatg cggttgtggg cggtctcgct gccgaggtgg
     6421 gcgaggcggg ccatcagacg taggttctcc tggccggtga gcagaccgtc gacggcggcg
     6481 aactgtcccg tgacgccgat ggacgcccgg acctgctcag cgtgcgagac gacatcgaac
     6541 ccggcgacgc ggaccgtccc ggcatccgcc gcgagcaggg tggtgaggat gttgatcgtg
     6601 gtcgtcttgc ccgcgccgtt ggctccgagc agtgcgaaca cgataccggc ggggatgtct
     6661 aggtctacgc cgcgcagcac gcggttgtcc ttgaacgatt tctgcagacc cctgatctgg
     6721 atcgcaggct gagtgggagc agacatggaa ggaatccttt ccacttgtgc ctcggtgcgg
     6781 aggccgaccg ggacgagccc agcagtttcg gcgcccgaca gctcggtgat gtcggtcgcc
     6841 cctttcaggg cgcgctcgat taccgtgcct cgccacacag ggaggcagcg gttacacgaa
     6901 tgcagcgttc agatcagtac gacggggtgc cgtggcggtt cggggagtgt cagcgcttcg
     6961 gcatctcctc cgcgtcggtg cctcgcaggc gggcgagcca ctcctccttg aggaggggat
     7021 acttctcggc gaggaagtcg ttcagcgccg cgagttgctc cagaccctga cgtcgacctg
     7081 agtcgtcgtc ctctgcgagc agagcaacac cccgccggat gagggcggcc tgctgggtgt
     7141 acccggccgc gtcgaaatcg cgctcgaaca gggacacatc gaagatggct gcgacccgat
     7201 ccactcgctc cccgcgtgcg cgggtgcggc gtgccatgtt ccgaccttcg aggagcacga
     7261 ccgcctgggt gatcgcgctt cggctcgcca gcagcgcgtc ggcgagatca ttgatggtct
     7321 gcgcggccgg attgcataca gcgagatacg ccagcacccg tcccgcgacg ggagggaagg
     7381 ccatctctcg aacgaagaac cggctcaggt cgtcacagaa cctcagttgc tcatcggtca
     7441 gcacaccaca acgatagcac ggatgactaa catgacacaa aaaagtgtta gaccagtcgt
     7501 ctatggtgtc cgggtgtgtc ggccctatga tcggctgtga gcgggcggtc accgactgtc
     7561 gtcacggtgc cgtgcatgag gactccggca gttcggtgga ggaatgttcg agacctgtgg
     7621 atgggccggg aagggcgtac cttcccgagt gatcgtgtag gtctcgagca aggccgacgt
     7681 cggcgcgtcg gtcggcaggg ccattgcgta gtcggg
//
LOCUS       DASYLX010000711         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1135639,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000711 DASYLX010000000
VERSION     DASYLX010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135639"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGP27_16755"
     CDS             complement(<1..530)
                     /locus_tag="VGP27_16755"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV7852983.1"
                     /translation="MASVDLNADLGESFGAWQLGDDDAMLGLVTSANVACGFHAGDPT
                     TLRRSCEEAAKQGVRIGAQVGYRDLAGFGRRFIDISSADLTADVIYQIGALQALAHVA
                     GSSVTYVKPHGALYNTIVNNHDQARAVAQAVHAVDPDLPVLGLAGSVFFAEADRLGLR
                     TVPEAFADRSYRPDGQL"
     gene            560..1387
                     /locus_tag="VGP27_16760"
     CDS             560..1387
                     /locus_tag="VGP27_16760"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV7852984.1"
                     /translation="MDRRRRDAVLITATELAALQAAGDPVAILDVRWQLTEPDGRDAF
                     ERGHVPGAVYVSLEDELSDHSVAGRGRHPLPSGRAVEAAGRRWGIRKDVPVVVYDDWN
                     RAGSARAWWVLTAAGIENVRILDGGLAAWTGELESGPVTPEPGDVSVPHEDLYAGALP
                     TLTAETVMAADTLIDARAPERFLGHVEPIDPVAGHIPGACNAPSTGFLAGDGTFLEER
                     DLARLFGDIDDAGAYCGSGVTASVVVAALATLGIDAALYPGSWSEWSSDAARPVETG"
     gene            complement(1384..>1795)
                     /locus_tag="VGP27_16765"
     CDS             complement(1384..>1795)
                     /locus_tag="VGP27_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV7852985.1"
                     /translation="VVTVVVTVVLAVCYRPLLFSTVDPEVAAARGVPVRAMGIVFAAL
                     VGVVAAQGVQIVGALLVMSLLITPAAAAARVFVSPTANIAASVVFAEISAVGGILLSL
                     APGVPVSVFVTTISFAIFLLCWLVGRRQRTVPAY"
BASE COUNT          292 a          599 c          656 g          248 t
ORIGIN      
        1 agctgtccgt cggggcggta ggaccggtcg gcgaacgcct cgggcacggt gcgcaggccg
       61 agccggtcgg cttcggcgaa gaacaccgaa cccgccaatc ccagcaccgg cagatcaggg
      121 tcgacggcgt gcacggcctg cgcaaccgcg cgggcctggt cgtggttgtt gacgatcgtg
      181 ttgtacagcg cgccatgggg tttcacgtac gtgaccgacg acccggccac gtgtgcgagc
      241 gcctgtagcg cgccgatctg gtagatgacg tcggcggtga ggtcggcaga actgatgtcg
      301 atgaagcgtc tgccgaaccc ggcgagatcg cggtagccga cctgggcgcc gatccggacg
      361 ccctgtttcg cggcttcttc gcagctgcgg cgaagcgtgg tcgggtcgcc cgcgtggaac
      421 ccgcatgcga cgttggcgct ggtgaccagg cccagcatcg cgtcgtcgtc gccgagctgc
      481 catgccccga agctctcccc gaggtcggcg ttgaggtcga cggaggccat gcagccaccc
      541 tagatggcac gctggacgcg tggaccgacg acggcgtgac gcggtattga tcaccgcaac
      601 cgaactggcc gcgctgcagg cggcgggcga tccggtggcg atcctggacg tgcgatggca
      661 gctgaccgag ccggacgggc gcgacgcctt cgaacgcggc cacgtccctg gtgcggtgta
      721 cgtatcgctg gaggatgagc tcagcgacca ctcggtcgcc gggcgcgggc gtcacccgct
      781 gccctcgggc cgcgcagtcg aggccgccgg gcgtcggtgg ggtattcgca aggacgtacc
      841 cgtcgtcgtc tacgacgact ggaacagggc cgggtcggcg cgggcatggt gggtgctcac
      901 cgccgcgggt atcgagaacg tccggatcct cgacgggggg ttggccgcct ggaccggcga
      961 gctggagagc ggaccggtga cgcccgagcc cggcgacgtc tcggtgccgc acgaggacct
     1021 gtacgccggt gcgcttccca ccctgacggc ggaaacggtg atggccgccg acacccttat
     1081 cgatgcgcgg gcacccgagc gattcctcgg ccatgtcgaa cccatcgatc ccgtcgcagg
     1141 gcacattccc ggcgcgtgca acgcgccgag caccggcttt ctggccgggg acggaacgtt
     1201 cctcgaggag agggatctgg cgcggctgtt cggcgatatc gacgacgcgg gcgcctactg
     1261 cggctccggg gtcaccgcgt cggtggtcgt ggccgcactc gcgacgctcg gcatcgacgc
     1321 tgcgctgtat cccgggtcgt ggtcagaatg gtcttccgac gcggcacgtc ccgtcgaaac
     1381 gggttagtac gccggcacgg tgcgctgccg cctgccgacg agccagcaca acaggaaaat
     1441 cgcgaacgag atcgtggtga cgaagaccga caccggcacc ccgggcgcca gcgacagcaa
     1501 gatgccgccg acggcggata tctcggcgaa gaccacggag gcggcgatgt tggcggtggg
     1561 ggagacgaaa acccgcgcgg ccgctgcggc gggcgtgatc aacagcgaca tcaccagaag
     1621 cgcgccgacg atctgtacgc cttgcgccgc caccactccc accagcgcgg cgaacacgat
     1681 gcccatcgcg cggaccggca cgccgcgcgc ggccgccacc tccgggtcga cggtggagaa
     1741 cagcagcggt cggtaacaga cggcaagcac gacggtcacg acgacggtga ccacg
//
LOCUS       DASYLX010000712         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2671269,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000712 DASYLX010000000
VERSION     DASYLX010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671269"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGP27_16770"
     CDS             complement(<1..390)
                     /locus_tag="VGP27_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEV7852986.1"
                     /translation="MHEDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCPVRPPF
                     ARNVRFYPTAAAAQRAGFRACKRCRPDASPGSPEWNVRGDVVARAMRLIADGTVDREG
                     VTGLASRVGYTTRQLERMLQAEVGANPL"
     gene            complement(530..679)
                     /locus_tag="VGP27_16775"
     CDS             complement(530..679)
                     /locus_tag="VGP27_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852987.1"
                     /translation="MEDELIKMGVDFTRGEDWQPYTVVDRNLYTGQNPASAAPLAKQI
                     LSALA"
     gene            complement(664..1137)
                     /locus_tag="VGP27_16780"
     CDS             complement(664..1137)
                     /locus_tag="VGP27_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7852988.1"
                     /translation="MARILFVVTGASYWTLNDGTRHPTDYWAEEFAAPYQAMTEAGHE
                     IVVATPGAVVGHVDVMSLRPTMAGSEQIALELEGILRTAEELRRPIELANVRLEDNGA
                     VYYPGGHGPMEDLWKDADSGRLLTAALASGSLPTPIPKRTRSACARKPSSPWKTN"
     gene            1314..1817
                     /gene="tpx"
                     /locus_tag="VGP27_16785"
     CDS             1314..1817
                     /gene="tpx"
                     /locus_tag="VGP27_16785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV7852989.1"
                     /translation="MAQITLRGNPINTVGELPPVGSPAPGFSLIGTDLGSVSSEQFRG
                     KPLLLNIFPSIDTPVCATSVRTFNERAATSGLTVVCVSKDLPFAQKRFCGAEGIENVL
                     TASAFRDGFGEDYGVTLVDGPMAGLLARAVVVVGANGDVLYSELVPEIAQEPDYDAAV
                     AASNPSA"
     gene            complement(1825..>3035)
                     /locus_tag="VGP27_16790"
     CDS             complement(1825..>3035)
                     /locus_tag="VGP27_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7852990.1"
                     /translation="RNAFVAPWRVARTILILWGVGTALLTVLYGMHDTDFIPRFLFAV
                     SFPGVLVATGCYMITEFALRPVAAQALEAGPAPHRLTDGIMGRTMLVWLLSSGVPVLG
                     IAVTAIFALSLHNLTETQFAYAVLGIAAATLVFGAILMWLLSWLTATPVRVVRSALKH
                     VEEGDLDCNVVVFDGTELGELQRGFNSMVEGLKERERVRDLFGRHVGREVAAAAEKER
                     PKLGGEERHVAVIFVDIIGSTQLVTGRSATEVVDLLNRFFAVIVEEVDRHHGFVNKFE
                     GDAALAVFGAPNHLDDPEDEALAAARAIAERIRAEVPECEAGIGVASGQAVAGNVGAK
                     ERFEYTVIGEPVNEAARLCELAKSTPGHLLASACTVEKASESERAHWTLGETVTLRGH
                     DEPTRLAVPV"
BASE COUNT          538 a         1015 c          961 g          521 t
ORIGIN      
        1 cagggggttc gcgccaacct cagcctgcag catccgctcg agctggcggg tggtgtagcc
       61 gacgcgagac gcaaggcccg tcacgccttc gcggtcgacg gtgccgtcgg cgatcagccg
      121 catcgccctg gccaccacgt cgccgcgcac attccattcc ggggagcccg gtgaagcgtc
      181 cggacggcag cgcttgcacg cccggaagcc ggcacgctgg gcggcggccg cggtgggata
      241 gaaccgcacg ttgcgcgcga aaggaggccg gacgggacaa ctgggcctgc agtagatcct
      301 cgtggtcagc acggcggtga cgaaccagcc gtcgaaccgt gcgtctttcg actgaacggc
      361 acggtagcag cggtcgaaat cctcatgcac gtctacgaca attacacgtc gccaccgaca
      421 tcactggcgg aaaaacgaca tcgacgtcgt gagtgcgaca gtcgacgcct cggacgctac
      481 ccatccgttg gctggggcgg ggccggtctc cgcggcgccg agccccgcat caggccaacg
      541 cactcaggat ctgcttagcc agaggcgcgg ccgacgcggg gttttggccc gtgtagagat
      601 tgcggtccac caccgtatat ggctgccagt cttcgcctcg cgtgaagtcg acccccatct
      661 tgatcagttc gtcttccacg gtgaagatgg ctttctcgcg caggccgacc gcgtcctctt
      721 cggaattggt gtaggcagtg acccggatgc gagcgcggcg gtcagcagtc gaccggaatc
      781 ggcgtccttc cataggtctt ccatcggacc gtggccgccg gggtagtaga cggcgccgtt
      841 gtcctccagg cggacgttcg ctaattcgat tgggcggcgc agctcctcgg cggttcgcag
      901 aatgccctcc agctccaggg cgatctgctc acttccggcc attgtggggc gcagactcat
      961 gacgtcgacg tggcccacta cagcgccggg ggtggcgacc acgatctcat gaccggcttc
     1021 ggtcattgcc tggtacgggg cggcgaactc ttcggcccaa taatcggtcg ggtgtcgggt
     1081 gccatcgttg agcgtccaat aactcgctcc ggtcacgacg aagaggattc tggccatcgt
     1141 tgcccctacc tacccggagg cccgtgagcc ggtctgctca gataatcggc cttcgcgtga
     1201 cgatcgaaat cgtacgctcg cggcttcctc gcagcgacag cacagcaagc gcgcggatcc
     1261 tcagtgcccg atgcaaagac agcgggccga tccgcctttg gcagactgtg tgcgtggctc
     1321 aaataactct gcgtggaaat cccatcaata cggttggcga gttgccgccc gtcggttcac
     1381 cggcgcccgg cttttcgttg atcggcaccg acctcggctc ggtcagcagc gagcagttcc
     1441 gtggaaaacc gcttctgctc aacatctttc cgtccatcga cacaccggtg tgtgccacca
     1501 gcgtgcgcac cttcaacgag cgtgccgcca cgagcggact caccgtcgtc tgcgtatcca
     1561 aggatctgcc gttcgcgcag aagcgattct gcggcgcgga gggcatcgag aacgtgctga
     1621 cggcgtcggc atttcgagac gggttcggcg aggattacgg agtcactctg gtcgacggtc
     1681 cgatggccgg tctgcttgca cgggccgtcg tggtggtcgg cgcgaacggt gacgtgctct
     1741 actccgagct cgtgcccgag atcgcccaag aacccgacta cgacgcagcg gttgccgcgt
     1801 ccaacccgtc ggcgtagtcc aacactagac cggaaccgcc agccgcgtcg gctcgtcgtg
     1861 tccgcgcagc gtcacggtct cgcccagagt ccagtgagca cgctcggact cgctcgcctt
     1921 ctcgacggtg caagctgacg cgagcaggtg gcccggtgtg gacttggcca gctcacagag
     1981 ccgggcagcc tcattcaccg gctcgccgat gacggtgtac tcgaagcgtt ccttggcgcc
     2041 gacgttgccg gccactgcct gcccggacgc cacgccgatt cctgcctcgc actcgggcac
     2101 ctcggcccgg atgcgctcgg cgatggcgcg agctgcggcc agcgcctcgt cctcgggatc
     2161 gtccagatgg ttcggcgcgc cgaagaccgc cagcgccgcg tcgccctcga acttgttgac
     2221 gaagccgtgg tggcggtcga cctcctcgac gatcaccgcg aagaaccggt tcaacaggtc
     2281 gaccacctcg gtggcggacc ggccggtcac caattgggtg gaaccgatga tgtcgacgaa
     2341 tatcaccgca acgtgacgtt cctcgccgcc gagctttggc cgctctttct ccgctgccgc
     2401 ggcaacctcg cggccaacat gcctgccgaa cagatcccgg acgcgctcgc gttccttgag
     2461 tccctcgacc atcgagttga aaccgcgctg tagctcgccc agttcggtgc cgtcgaaaac
     2521 cacgacgttg caatccaaat cgccctcctc gacatgcttg agcgccgaac gcaccacccg
     2581 caccggcgtc gcggtaagcc acgacaacag ccacatcagg atggcgccga agacaagggt
     2641 cgcggccgcg atacccaata cggcgtaggc gaattgagtc tcggtgaggt tatgcaacga
     2701 caacgcgaat atcgcggtca cggcgatgcc gagcaccggt acaccggagc tgagcagcca
     2761 caccagcatc gtcctgccca tgattccgtc ggttaggcgg tgaggcgcgg gaccggcttc
     2821 cagtgcctgt gcggcaactg ggcggagagc gaattcggtg atcatgtagc agccggtagc
     2881 caccaacaca ccgggaaagc tgaccgcaaa caggaaccgc gggatgaaat ccgtgtcgtg
     2941 cattccgtat aggaccgtca gcagcgccgt gccgacgccc cagagaatca agatcgtgcg
     3001 ggccacccgc cacggcgcca caaaggcgtt gcggt
//
LOCUS       DASYLX010000713         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3331260,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000713 DASYLX010000000
VERSION     DASYLX010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331260"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="sufB"
                     /locus_tag="VGP27_16795"
     CDS             complement(<1..1127)
                     /gene="sufB"
                     /locus_tag="VGP27_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV7852991.1"
                     /translation="MTTTPEALTQEQTIESLGRYGYGWSDSDVAGASAQRGLSEAVVR
                     DISAKKSEPEWMLDVRLKALRVFDKKPMPNWGSNLEGIHFDNIKYFVRSSEKQAATWD
                     DLPADIKNTYDRLGIPEAEKQRLVSGVAAQYESEVVYHSIREDLEKLGVIFLDTDTAL
                     KEHPELFKQYFGTVIPAGDNKFSALNTAVWSGGSFIYVPKGVHVDIPLQAYFRINTEN
                     MGQFERTLIIVDEGAYVHYVEGCTAPVYKSDSLHSAVVEIVVKPGGRCRYTTIQNWSN
                     NVYNLVTKRARAEAGATMEWIDGNIGSKVTMKYPAVWMTGEHAKGEVLSVAFAGEGQH
                     QDTGAKMLHLAPNTSSNIVSKSVARGGGRASYRGLVQINKGA"
     gene            complement(1124..1876)
                     /locus_tag="VGP27_16800"
     CDS             complement(1124..1876)
                     /locus_tag="VGP27_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7852992.1"
                     /translation="MKFSPEAGAALSATSGPDRHTRGAIIQLLLESGSITASEIGESL
                     GISAAGVRRHLDALLEAGDAQSSAAAAWQHNGRGRPARRYRLTAAGRAKLGHAYDDLA
                     AAAMRQLREIGGDDAVRTFARRRIDTILSGVTEGSDDVESTVDRVAGALTKAGYAATA
                     TQVSGPLHGIELCQHHCPVSHVAKEFPELCETEREAFAEVLGTHVQRLATIVNGDCAC
                     TTHVPLNQGQQPTPRRTAHGAEAIKTKQGAST"
     gene            1953..>2193
                     /locus_tag="VGP27_16805"
     CDS             1953..>2193
                     /locus_tag="VGP27_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV7852993.1"
                     /translation="MSEQNRRLVLAQRPSGLVNEATTRRETETLPELQDGEALVKVRY
                     LSIDPTIRTWMDDVPSYLPPIQIGEVVRSGGVGEVI"
BASE COUNT          359 a          706 c          708 g          420 t
ORIGIN      
        1 gcacccttgt tgatctgaac gagaccacgg taggacgccc ggccgccgcc acgggcaacc
       61 gacttggaga cgatgttgct cgacgtgttc ggcgccaggt gcagcatctt ggcgccggtg
      121 tcctggtgct ggccctcgcc ggcgaacgcc accgagagca cctcgccctt cgcgtgctcg
      181 ccggtcatcc acactgccgg gtacttcatc gtgaccttgg acccgatgtt gccgtcgatc
      241 cactccatgg tggcgccggc ctccgcgcgt gcccgcttgg tgaccaggtt gtagacgttg
      301 ttcgaccagt tctggatcgt ggtgtaacgg caacggccac ctggcttcac gacgatctcc
      361 acgacagccg agtgcagtga gtccgacttg taaacgggag cagtgcagcc ctccacgtaa
      421 tgcacatacg cgccctcgtc gacgatgatc agcgtccgct cgaactggcc catgttctcg
      481 gtgttgatcc ggaagtaggc ctgcagcgga atgtcgacgt gcacgccctt gggtacatag
      541 atgaacgagc cgcccgacca gacagcggta ttcagcgcgg agaacttgtt gtcacctgcc
      601 gggatgaccg tgccgaagta ctgcttgaac agctcgggat gctccttcaa ggcggtatcg
      661 gtgtcgagga agataacgcc cagcttctcc agatcctcgc ggatggagtg gtagacgacc
      721 tcggactcgt actgagccgc gacgccggac accaggcgct gcttctcggc ttccgggatg
      781 ccgagcctgt cgtaggtgtt cttgatgtcg gcaggcaggt catcccacgt ggcggcctgc
      841 ttctcacttg accgcacgaa gtacttgatg ttgtcgaagt ggatgccctc gagattggag
      901 ccccagttcg gcatcggctt cttgtcgaag acgcgcagcg ccttgagtcg gacgtcgagc
      961 atccactccg gttcactctt cttggccgaa atgtcacgca caacggcctc cgacagcccg
     1021 cgctgcgcgc tcgcgcccgc aacgtcggag tcggaccagc catagccgta gcgtcccagc
     1081 gactcgatgg tctgttcctg ggtcaaggcc tcgggcgtgg tcgtcatgta gacgctcctt
     1141 gcttggtttt gatggcttcg gcgccgtggg ctgtacgccg aggggttggt tgctgtccct
     1201 ggttgagcgg cacgtgcgtg gtgcaggcgc agtctccgtt gacgatcgtg gccagccgct
     1261 gcacgtgagt acccagcacc tcggcgaacg cttcccgttc tgtctcgcac aactccggaa
     1321 actccttggc gacgtgcgac accgggcagt gatgctggca gagctctatg ccgtgcaggg
     1381 gtccgctcac ctgggtggcg gtagcggcgt acccggcctt ggtcagtgca cctgccacgc
     1441 gatcgacagt tgattcaacg tcatcggatc cctcggttac tcccgacagg atggtgtcga
     1501 tacggcgccg cgcgaaggtg cggacggcgt cgtcgcctcc gatttcacgt agttggcgca
     1561 tggctgcggc ggcaaggtcg tcgtaggcgt ggccgagctt ggcgcgcccc gcggcggtca
     1621 gtcggtaccg ccgagcgggc ctgccgcgac cgttgtgctg ccaggccgcg gctgcactgg
     1681 actgcgcatc gccggcctcc agcagcgcgt ccaggtgcct gcgaacgccc gccgcggaga
     1741 tgccgaggct ctcgccgatc tcgcttgcgg tgatggatcc ggactccagc aagagctgga
     1801 tgatcgcgcc acgcgtgtgg cggtcgggcc cggatgtcgc cgacagggcg gctccggcct
     1861 ccggactgaa tttcacaaca ccattgtgac ggaattccga atcccggtaa agcaaggcca
     1921 cccttatgtg ggcaacgtcc tacgatcgcc acgtgagcga gcagaatcgg cgactggtcc
     1981 tggcccaacg accctccggc cttgtcaacg aggccacgac acggcgggag accgagacct
     2041 tgcctgaact gcaggacggc gaggccctgg tcaaggtccg ctacctctcg atagatccga
     2101 cgatccgcac ctggatggac gacgtgccga gctatctgcc gccgatccag atcggcgaag
     2161 tcgtccgcag cggcggcgtc ggcgaggtca tcg
//
LOCUS       DASYLX010000714         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3000862,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000714 DASYLX010000000
VERSION     DASYLX010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3000862"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VGP27_16810"
     CDS             <1..913
                     /locus_tag="VGP27_16810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7852994.1"
                     /translation="MTRTDDDSWDITESVGATALGVAWARSQESTSQCPLFTDPFAEM
                     FVDEAIARGWQMPQRYVAERIRSIAGYTGSRTKWFDEFFIAAGAGGIDQAVILAAGLD
                     ARAWRLPWNDGSVVYEIDQPKVLAFKTETLHSRGIEPATRYVAVPGDLRHDWPKALRE
                     AGFDPSEPTAWAAEGLLPYLPAAGQDLLFERIADLSAQGSRVAIEAFGAGFFDPEYLA
                     GRRERMRKMREEAGVPDETPDVADLWFIEDRTEVTEWFSAQGWEVSAIDAADLMKRFS
                     RTTDDDTTPRTVFVEARLNAAAGRLRS"
     gene            complement(918..1553)
                     /locus_tag="VGP27_16815"
     CDS             complement(918..1553)
                     /locus_tag="VGP27_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV7852995.1"
                     /translation="MPTIPMFPLERAMLPGEELPLRIFEPRYSAMISDCLAMDDPAFG
                     VVLIAAGREVGGGDARSDVGTLAHITEVADFGDGRYRLNAVMAERIRVLEWQPDNPYP
                     RAAVEMWPDEPGEPVGPDAVRDVEDRMIALFERIASARGAEVSGRDIVAGADAAPDAA
                     MWLYALTTRLPMGQADRYSVLAAPTNAARVAALNDAVETVSALVEFQLSGE"
     gene            complement(1556..2686)
                     /locus_tag="VGP27_16820"
     CDS             complement(1556..2686)
                     /locus_tag="VGP27_16820"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV7852996.1"
                     /translation="MLSAPVSSRIDFAGSARPTVGVEWEFALVDAETRDLSNEAAAVI
                     AEIGENPRVHKELLRNTVEVVTGICDNAGQAMEDLRSTLLPARQVVHDRGMELFCAGT
                     HPFAKWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGVSSAHKVMPIVTSLLNQ
                     YPHLLALSSSSPYWDGVDTGYASNRAMMFQQLPTAGLPFHFETWAQWEGFVHDQKKTG
                     IIDHMNEIRWDIRPSPHLGTVEVRIFDGVSNLRELGALTALTHCLIVDLDRRLEAGEQ
                     LPTMPPWHVQENKWRAARYGLDAVIILDADSNERLVTEDLDALLNRLEPVAKSLGCAD
                     ELASVADIYRLGASYQRQRRVAEDSDGDLRAVVDALVGELDI"
     gene            complement(2667..>2993)
                     /locus_tag="VGP27_16825"
     CDS             complement(2667..>2993)
                     /locus_tag="VGP27_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEV7852997.1"
                     /translation="GEPGDFNSAGGHFQVPGHSGHPASGDLTSLQVREDGTAQLVTTT
                     DAFTSEDLLAGAKTSIIIHEKADNFANIPPERYQQINGTPPPDPTTLATGDAGKRVAC
                     GVIGTG"
BASE COUNT          497 a         1003 c         1004 g          489 t
ORIGIN      
        1 aatgaccaga actgacgacg actcctggga catcaccgaa agtgtcggcg cgactgcact
       61 cggcgtggcg tgggcgcgtt ctcaagagtc cacgtcgcag tgtccgctgt tcaccgatcc
      121 gttcgcggag atgttcgtcg acgaggccat cgcgcgcggt tggcagatgc cgcagcgtta
      181 cgtggccgag aggatccgct ccatagcggg atacaccggg tcgcgcacga aatggttcga
      241 cgagttcttc atcgcagcgg gcgcgggcgg catcgaccag gcggtgatcc tcgccgcggg
      301 actcgacgcc agggcgtggc ggctgccctg gaacgacggc agcgtcgtct acgagatcga
      361 ccagccgaag gtgctggcgt tcaagaccga gacgctgcac agccgcggca tcgaacccgc
      421 aacccgttac gtggcggtac ccggcgacct tcggcacgac tggccgaagg cgttgcgcga
      481 ggccggattc gacccgagcg agccgacggc atgggcggcc gaggggttgc tgccctacct
      541 gcccgcagcc ggccaggatc tgctgttcga gcgcatcgcc gatctcagcg cgcagggcag
      601 ccgcgtcgcg atcgaggcct tcggagccgg cttcttcgac ccggagtacc tggccggccg
      661 ccgggaacgg atgcggaaga tgcgcgaaga ggcgggtgtg cccgacgaga cacccgacgt
      721 tgcggatctg tggttcatcg aggaccgcac cgaggtcacc gaatggttct cggcgcaagg
      781 ctgggaagtc tcggcgatcg atgccgcgga tctgatgaag agattcagcc gcacgaccga
      841 cgacgacaca acgccgcgaa ccgttttcgt cgaggccaga ctcaatgccg ccgccgggcg
      901 gcttcgatcc tgaccggtta ctcgcccgac aactgaaact cgaccagcgc gctgacggtt
      961 tcgacggcgt cgttcagcgc ggccacccgc gccgcgttgg tcggcgcggc cagcaccgaa
     1021 tacctgtcgg cctgccccat cggcagccgg gttgtcaacg catacaacca catcgccgcg
     1081 tcgggcgccg cgtcggcgcc tgccacgata tcccgtccgc tgacctcggc accgcgggcg
     1141 ctcgcgatcc gttcgaagag cgcgatcatg cggtcctcga cgtcgcggac ggcgtcgggc
     1201 ccgaccggtt cacccggctc gtcgggccac atctcgacag cggcccgtgg ataaggattg
     1261 tcgggttgcc attccagcac cctgatacgt tcggccatca cagcgttgag ccggtaccgg
     1321 ccgtcgccga aatcggcgac ctcggtgata tgcgccagcg tcccgacatc gctgcgagcg
     1381 tcgccaccac cgacctcacg tccggccgcg atgagcacga cgccgaacgc cgggtcgtcc
     1441 atcgcgaggc aatcggagat catcgccgaa taccgcggtt cgaagatccg cagtggcaac
     1501 tcctcgcccg gcagcatggc ccgctccagc ggaaacatcg gaatggtggg cacggctaga
     1561 tgtccagctc accgaccaac gcgtcgacca ccgcgcgcag gtcgccgtcg ctgtcttcgg
     1621 cgacgcgccg ctgacgctga taggacgcac ccagccggta gatgtcggcg accgaggcca
     1681 gctcgtcggc acagccaagg gacttagcca ccggctcgag ccggttgagc agcgcgtcga
     1741 ggtcctcggt gaccaaccgc tcgttgctgt cggcgtccag gatgatcacc gcgtcaaggc
     1801 cgtaacgcgc tgcgcgccac ttgttttcct gcacatgcca cggcggcatt gtcggcagct
     1861 gttcaccggc ctccaaccgt cgatccaggt cgacgatcag gcagtgggtc agcgcagtca
     1921 gcgcgccgag ctcgcggagg ttggagacgc cgtcgaagat gcggacctcc accgtgccga
     1981 ggtgcggcga aggccggatg tcccaacgga tctcgttcat gtggtcgatg atgccggtct
     2041 tcttctggtc gtgaacgaag ccctcccact gcgcccaggt ctcgaagtgg aacggcaggc
     2101 ccgccgtcgg cagctgctgg aacatcatcg cccggttgct cgcatacccc gtgtccacgc
     2161 cgtcccagta gggcgacgac gacgacagcg cgagcaggtg cggatactgg ttcagcagcg
     2221 aggtgacgat cggcatgacc ttgtgcgcgg acgagacgcc gacgtgcacg tgcacgcccc
     2281 agatcagcat ctggcggccc caccactgtg tgcgcttgat cagctccgcg taacgcggag
     2341 cgtcggtcaa cttctgcgcc gaccacttcg cgaacggatg cgtgcccgcg cagaacaact
     2401 ccatgccgcg gtcgtgcacg acctggcgcg ccggcagcag ggtggaccgc aggtcctcca
     2461 tcgcctgacc cgcgttgtcg cagatgccgg tgacgacctc gacggtgttg cgcagcaatt
     2521 ccttgtggac gcgcgggttt tcaccgatct cggcgatcac cgcggcggcc tcgttactga
     2581 ggtcgcgggt ttcggcgtcg acgagggcga actcccactc gacgccgacg gtcggccggg
     2641 cggacccggc gaaatctatc cgactgctaa ccggtgccga taacaccgca cgcgacccgc
     2701 tttccggcgt cgccggtagc cagcgtggtt gggtcgggag gcggagtgcc gttgatctgc
     2761 tggtagcgct ccggcgggat gttggcgaag ttgtcggcct tctcgtggat gatgatcgac
     2821 gtcttggcgc ccgcgagtaa atcctctgaa gtgaaggcgt cggtcgtggt cacgagttgg
     2881 gcggtgccgt cctcgcgcac ctgcagcgac gtgaggtcac cgctggcggg gtgtccgctg
     2941 tggcccggca cctggaaatg tcctccggcg gagttgaagt cgcccggttc gcc
//
LOCUS       DASYLX010000715         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1354991,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000715 DASYLX010000000
VERSION     DASYLX010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354991"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGP27_16830"
     CDS             complement(<1..920)
                     /locus_tag="VGP27_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7852998.1"
                     /translation="MVAATLALTMAVLLVAAVLLGRSPEPAGKTVVTVRLWDEQVAAA
                     YRESFDEFTRLHPDIDVRVNVVAYATYFDTLRTDAAGGSADDIFWLSNAYFTQYADSG
                     HLMDIAKTLGRKAATGWDPSVAGQFTRNGVLWAVPQLTDAGIAVFFNADLVKAAGVDL
                     RQLLAMRWSPGDVDTLRPLLARLTVDADGRRADEPGFDAGRIRQWGYNAANDLQGIYL
                     NYIGSAGGMFSDGDRFAFDNPGSVAAFDYLVRLINDDHVAPPASDTNGNGDFSRNQFL
                     QGRMALFQSGTYNLAAIAEQSRFRWGVVPMP"
     gene            complement(944..1771)
                     /locus_tag="VGP27_16835"
     CDS             complement(944..1771)
                     /locus_tag="VGP27_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7852999.1"
                     /translation="MTSFSRAASAPVIYAGLAAGALITLAPFALGLLTSFTSAEQFNT
                     GTPLSWPSPPTVDNYVGLADAGFGRALVVTALMTAVILVGQLTFSVLAAYAFARLEFP
                     GRDALFWLYLATLMVPATVTVVPLYLMMAQAGLRNTFWALVLPFVFGSPYAIFLLREY
                     FRGIPADLIKAARIDGANTLDVLVHVVLPAGKPILVTLSLITVVSQWNSFMWPLVITS
                     GGKWQVLTVATAGLQSRYNAQWTLVLAATTVAIVPLVLLFIALGRHIVRSIVVTGLK"
     gene            complement(1758..2630)
                     /locus_tag="VGP27_16840"
     CDS             complement(1758..2630)
                     /locus_tag="VGP27_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7853000.1"
                     /translation="MAVNRVRTTAVGYALLAPSLFGVVTFLLLPMLVVVWLSVHRWDL
                     LGSIQYVGLDNWRSVLTDSSFATSLVVTALFIVIVVPAQAVLGLIAAAMLAPGLPGSG
                     FFRTLYVLPWISAPLAIAVLWRWLLAPTDGAVSTLLGHRVEWLTDPDLALPVVSAVTI
                     WTNVGYVTLFFLAGILAIPRDIQNAARTDGANAWQRFRRITLPMLRPTLFFVLVTGVI
                     SAAQVFDTVYALTAGGPQGRTDLIAHRIYAEAFGAAAVGRASVMAAVLFVLLVGVTVV
                     QHLYFRRRITYDVV"
     gene            2676..4193
                     /locus_tag="VGP27_16845"
     CDS             2676..4193
                     /locus_tag="VGP27_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEV7853001.1"
                     /translation="MSAPRQVDPDFLELPRRQLADAALSAALAAGASHADLRIHRVVN
                     EVLALRDGELETSVVDREVGFAVRVIVDGTWGFASNAELDAGVAAETARRAVAVARTL
                     APLNAETIALAPEPVYVEVSWVSDYRIDPFSIAAADKIGLLAEYSGRLLAADGVNHVS
                     ASLHAVKEQTFYADTAGSSITQQRVRVQPTLEAVTVDAAAGSFETMRTLAPPTGRGWE
                     AVEGDDVWDWSNELAELPTLLTEKVKAPSVTPRPTDLVIDPSNLWLTIHESIGHATEY
                     DRAIGYEAAYAGTSFATPDKLGTMRYGSRVMNVTADRTENFGLATVGWDDEGVQAQRW
                     DLVRDGTFVGYQLDRVFAPRLGIARSNGCSYADSPHHVPIQRMANVSLQPGVDGLSTD
                     DLVARVDDGIYIVGDKSWSIDMQRYNFQFTGQRFFRITDGKLDGQLRDVAYQATTTDF
                     WGAMEAIGGPSTWRLGGAFNCGKAQPGQVAPVSHGCPSALFRGINVLNTRTEGGR"
     gene            4196..>5177
                     /locus_tag="VGP27_16850"
     CDS             4196..>5177
                     /locus_tag="VGP27_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV7853002.1"
                     /translation="MISAQQVVDIVLAEAARLGKADETIVLVTDRSDAALRWANNSMT
                     TNGVSVQRRTTVISVVRRGDRGHVASLTSSEVDPAVIPALVAASQDAALSAPEARDSA
                     PPLPAGEAANDWDAPIPGTGVDVFGGVAETLTRGFARADRLYGYARHILETTFVATSY
                     GLRRRYTQPTGSVEINAKRDGASAWAGTSTPDFVDVPTDSMLDELSTRLGWARNFVEL
                     PAGRYETILPPSAVADLMIYMSWSMDGRGAQEGRTAFSAPGGGTRVGEKVTDLPLTVY
                     SDPAAAGLECLPFVATPSSSERVSVFDNGMDIARVDWIRDGTINALAYPRA"
BASE COUNT          919 a         1733 c         1774 g          751 t
ORIGIN      
        1 ggcatcggga cgacgcccca ccggaagcgg ctctgctcgg cgatcgccgc caagttgtag
       61 gtgccggact ggaacagcgc catcctgccc tgcaggaact gattgcggga gaagtcgccg
      121 ttgccgttgg tgtccgacgc cggcggcgcg acatggtcgt cattgatcag acgcacgagg
      181 taatcgaacg ccgcgacgga cccggggttg tcgaacgcga accgatcgcc gtcggagaac
      241 atcccgcctg ccgagccgat gtaattgagg tatatgcctt gaagatcgtt ggcggcgttg
      301 tacccccact gccgtatgcg gcccgcgtcg aagccgggtt cgtcggcccg tcgtccgtcg
      361 gcgtcgacgg tcaaccgggc gagcagcggc cgcagggtgt cgacatcgcc gggggaccag
      421 cgcatcgcga ggagttgccg cagatccacg cccgcggcct tgacgaggtc ggcgttgaag
      481 aaaaccgcaa tgcccgcatc tgtcagctgc ggcaccgccc acagcacgcc gttacgggtg
      541 aactggccgg ccaccgacgg atcccagccc gttgcggcct tgcgccccaa cgttttcgcg
      601 atgtccatca ggtgaccgct gtcggcatac tgcgtgaagt acgcattcga cagccagaag
      661 atgtcgtcgg cgctgccgcc cgcggcgtcg gtgcgcagcg tgtcgaaata ggtggcgtac
      721 gccaccacgt tgacccgtac gtcgatgtct ggatggaggc gggtgaactc gtcgaacgat
      781 tcgcggtatg cggccgcgac ctgttcgtcc cacagacgca cggtgaccac cgtcttgccc
      841 gcgggctccg gcgatcggcc cagcagcacc gccgccacca gcagcacggc catcgtgagc
      901 gccagtgtcg ctgcgaccat cgtggagtat ttcggacgga cgctcacttg agccccgtca
      961 ccacgatgga gcgcacgatg tgcctgccga gtgcgatgaa cagcaggacg agcgggacga
     1021 tcgccaccgt cgtcgcggcc agcaccaacg tccactgcgc gttgtagcga gactgcaggc
     1081 cggccgtcgc caccgtcagc acctgccact tgccgccgct ggtgatcacc agcggccaca
     1141 tgaagctgtt ccattggctg acaacggtga tcagcgacag cgtcaccaga atcggcttgc
     1201 cggcggggag cacgacgtgc accaggacgt cgagggtgtt ggctccgtcg atgcgtgccg
     1261 ccttgatgag atcggcaggg atcccgcgga agtactcgcg cagcaaaaag attgcgtacg
     1321 gcgagccgaa cacgaacggc agcaccaacg cccagaacgt gttgcgcaga ccagcctgcg
     1381 ccatcatcag gtacagcggc accaccgtca ccgtggcggg caccatcaac gtcgcaaggt
     1441 acagccagaa caacgcgtcg cggccgggga actcgagtcg cgcgaacgca tatgccgcaa
     1501 gcaccgagaa cgtcagttgg ccgaccagga tgacggcggt catcagcgcc gtcaccacga
     1561 gcgcgcggcc gaacccggca tcggccaggc cgacgtagtt gtccaccgtc ggcggactcg
     1621 gccacgacag cggcgtgccg gtgttgaact gctccgcgga cgtgaacgat gtcagcagcc
     1681 cgagggcgaa cggcgccagc gtgatcaacg cgcccgcggc caacccggcg tagatgaccg
     1741 gcgccgacgc ggcccggcta aacgacgtca taggtgatcc gcctgcggaa gtagagatgc
     1801 tggaccaccg tcacaccgac cagcaggacg aacaacaccg cggccatcac cgacgcccgc
     1861 ccgaccgcgg cggcgccgaa cgcctcggca tagatgcgat gagcgatcag gtcggtgcgg
     1921 ccctgcggtc cgcccgcggt gagcgcatac acggtgtcga agacctgcgc cgcactgatg
     1981 actccggtga cgaggacaaa gaacagcgtc ggccgcagca tcggcaatgt gatgcggcga
     2041 aagcgttgcc acgcgttggc gccgtcggtg cgtgccgcgt tctggatgtc gcggggaatc
     2101 gcgaggatgc ctgcgaggaa gaacagcgtc acgtaaccga cgttggtcca gatcgtcacg
     2161 gccgacacca cgggcagcgc caggtccggg tcggtcagcc actcgacccg gtgccccagc
     2221 agcgtgctca ccgcaccgtc ggtcggggcc agcaaccacc gccagagcac cgcaatcgcc
     2281 agtggcgcgc tgatccacgg cagcacgtat aacgtgcgga agaacccgct gccgggcagt
     2341 ccgggggcca gcatggcggc ggcgatgaga ccgagcaccg cctgggcggg cacgacgatc
     2401 acgatgaaca gcgccgtcac caccagcgac gtggcgaacg atgagtccgt cagcacagaa
     2461 cgccaattgt ccagtccgac atactgaata gaccccagca ggtcccagcg gtgcacgctg
     2521 agccagacga ccaccagcat cggcagcagc aggaaagtga cgacaccgaa caggctgggc
     2581 gccagcagcg cgtaccccac tgccgttgtg cgcacccgat tgaccgccac cgagccatta
     2641 aagcgggcgc ggcgcggtcg aactacggtg tagccgtgag cgcaccccgc caagtcgacc
     2701 ccgacttcct cgagctgccc cgccgtcagc tcgccgacgc cgcgctttct gctgcccttg
     2761 cggccggcgc gagccacgcc gacctgcgca tccaccgcgt cgtcaacgag gttctggcgc
     2821 tgcgcgacgg cgagctggag acctccgtcg tcgaccgcga ggtcggtttc gcggtgcggg
     2881 tgatcgtgga cggcacgtgg ggcttcgcgt cgaacgccga gctggatgcc ggtgtcgcgg
     2941 cggaaacggc tcgccgcgcg gttgcggtgg cgcgcacatt ggcaccgctc aacgcggaga
     3001 cgatcgcgct ggcccccgaa ccggtgtacg tagaggtcag ctgggtgtcc gactaccgga
     3061 tcgacccgtt ctcgatcgcg gcggccgaca agatcgggct gctcgcggag tattcgggcc
     3121 ggctgctggc cgccgacggc gtgaaccacg tgtcggccag cctgcacgcg gtcaaggagc
     3181 agacgttcta cgccgacacg gcgggctcct cgatcaccca gcagcgggtg cgggtgcaac
     3241 cgacgctgga ggcggtgacc gtcgacgcgg cggcggggtc gttcgaaacc atgcgcacgt
     3301 tggcgccgcc gaccgggcgc ggatgggagg ccgtcgaggg cgacgacgtg tgggactggt
     3361 cgaacgagct cgccgaactg ccgacgctgc ttacggagaa ggtcaaggcg ccgagcgtga
     3421 caccgaggcc gaccgacctc gtcatcgatc cctccaacct gtggctgacc atccatgaat
     3481 cgatcgggca cgccaccgaa tacgaccgcg cgatcggtta cgaggccgct tacgcgggta
     3541 cgtcgttcgc cacgccggac aagctgggca cgatgcgcta cggctcgcgg gtgatgaacg
     3601 tgaccgccga ccgcaccgag aacttcgggc ttgcgacggt cggctgggac gacgagggcg
     3661 tgcaggcgca gcggtgggat ctggtgcgcg acggtacctt cgtcggctac cagcttgacc
     3721 gggtgttcgc gccgcggttg gggatcgcgc ggtccaacgg ctgctcgtac gccgactccc
     3781 cgcatcacgt gccgatccag cggatggcca acgtctcgct gcagcccggc gtcgacggcc
     3841 tgagcaccga cgacctcgtt gcgcgggtcg acgacggcat ctacatcgtc ggcgacaaga
     3901 gctggtcgat cgacatgcag cggtacaact ttcagttcac cggacagcgg ttcttccgga
     3961 tcacggacgg caagctcgac gggcaactgc gcgatgtggc gtatcaggcc accaccaccg
     4021 acttctgggg tgcgatggaa gccatcggcg gtccgtcgac gtggcggctg ggcggcgcgt
     4081 tcaactgtgg gaaggcgcaa cccggacagg tggccccggt cagccacggc tgcccgtccg
     4141 cgttgttccg cgggatcaat gtgctcaaca cccgcaccga aggcggacgc taacggtgat
     4201 cagtgcacag caggtcgtcg acatcgtatt ggccgaggcg gcacggttgg gcaaggccga
     4261 cgaaacgatc gtgctggtca ccgaccgcag tgacgccgca ctgcggtggg cgaacaattc
     4321 gatgaccacc aacggtgttt cggtgcagcg caggaccacg gtgatctccg tggtgcgtcg
     4381 cggcgaccgc ggccacgtgg cgtcgctgac atccagcgag gtcgatccgg cggtcatccc
     4441 cgcactggtg gcggcctcgc aggacgcggc gctgtcggcg ccggaagccc gcgacagcgc
     4501 gccaccgctg cccgcggggg aggcggcgaa tgattgggac gcaccgattc cgggcaccgg
     4561 tgttgatgtc ttcggcggtg tcgcagaaac gctgacgcgc ggctttgccc gcgccgaccg
     4621 gttgtacgga tacgcacgcc acatcctgga gacgacgttc gtcgcgacgt cgtacggact
     4681 gcggcggcgc tacacccaac cgaccgggtc ggtcgagatc aacgccaagc gcgatggagc
     4741 cagcgcctgg gcgggcacca gcacgcccga cttcgtcgac gtgccaaccg attcgatgct
     4801 cgacgagctc tcgacgcggc tcgggtgggc gcggaatttt gtcgagcttc ctgcggggcg
     4861 ctacgagacg atcctgccgc cgtcggccgt cgccgacctg atgatctaca tgtcgtggtc
     4921 gatggacggc cgtggagcgc aggagggccg caccgccttc tcggcgcccg gcggcggcac
     4981 gcgggtgggg gagaaggtca ccgacctgcc gttgactgtg tactccgatc ccgcggccgc
     5041 aggcctggaa tgcctgccgt tcgtagccac gcccagttcc tcggagcggg tctcggtgtt
     5101 cgacaacggc atggacatcg cccgggtgga ctggatccgc gacggcacga tcaacgcgct
     5161 ggcctatcca cgggccg
//
LOCUS       DASYLX010000716         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_917339,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000716 DASYLX010000000
VERSION     DASYLX010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917339"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1111
                     /locus_tag="VGP27_16855"
     CDS             113..1111
                     /locus_tag="VGP27_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853003.1"
                     /translation="MRETAVREGHQIFTNELARFAEQTGDPRIIALVDEVQGPLRVAV
                     RGRDGVGRGTVESALAGAGITVADDATADVDVVVVAEALKPEDRAMPRGRPTLLVLNK
                     ADLAGFGAGGPIAVADRRAAEIRALTGVPTVSMVGLLAVAAMDDELVAALQTLTAEPA
                     DLTSTDRFLSAGHSLARDVRARLLDTLDLFGIAHCVLALRQGTDGAALPVVLRRLSRI
                     DPVVARIAATGAEVRYRRVRSALVELRTMAARGTPAVTQFLSGDDAVLAAMAAAVDVV
                     QAEGLTVDAEDQPSAHLRRALHWHRYSRGPVNPIHRDCGADICRGSLRLLRRTGPR"
     gene            1108..2559
                     /locus_tag="VGP27_16860"
     CDS             1108..2559
                     /locus_tag="VGP27_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853004.1"
                     /translation="MTDVDQAGDPIGAVDALVAGVDPGLSSPGVEHRDVVLVTGPWLA
                     GATGLITALRDRLPDVEFVESEEIRSADAPAAVVFVVSAVAPIAESDCALIDLAAQHT
                     DLVIGAVSKIDVHREWRDVLAEDRSRLAAHAGRYRDVPWVGVAAAPDLGEPNVEEIVE
                     LLRSPLDDADVKRRNRLRAWESRLQTVIGRHDKDGAGGDRQARVTALRERRDEVLRNR
                     RVSKSERTIALRSQIQQARVQLAYFARNRCTSVRSELQDDASAMTRRRLPEFEAYVGT
                     RVSEVVSEVEAGITRHLGDVATELELTAPVPPPPPTPPAVSPPPLKSRRLETRLMMVL
                     GAGFGLGVALGISRLFAGLAPGLTIAGLVAGGVVGLLLTVWVVGMRGVLSDRSVLDRW
                     ITELTGTLQSAVEERVATRVLAAEATLTAEVARRDEADAEAAAGQVDEIDAELREHAI
                     ATARAAALRDRRLPPLQKALETVRAQLAGGPQD"
     gene            2726..>3285
                     /locus_tag="VGP27_16865"
     CDS             2726..>3285
                     /locus_tag="VGP27_16865"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214725.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEV7853005.1"
                     /translation="MTSATIPGLDTAPTKHAGLLGWVQEVAELTQPDRVVFADGSDEE
                     NARLCEQLEAAGTFKKLDETKKPNSYLALSDPSDVARVESRTFICSEREIDAGPTNNW
                     MDPAEMRGIMTDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMT
                     RMGKAALEKMGEDGFFVKALHSIGAPL"
BASE COUNT          515 a         1036 c         1207 g          527 t
ORIGIN      
        1 aaagtgcgag aagcgatcgc catttcgtcg atctcggtgc gcgccgctgc ccgaaagatc
       61 cgttcgcggt gtacccacgg agatgccacc atttgcggtg gggggcagac acgtgcgcga
      121 aacggcagtc agggaaggcc accagatttt caccaacgaa ctggcacggt tcgctgaaca
      181 aaccggtgat ccgcggataa tcgcgctcgt cgacgaggtt caaggcccgc tcagggttgc
      241 ggttcgcggg cgtgacggcg tgggtcgggg gaccgtggaa agcgcgctcg caggtgcggg
      301 gatcaccgtc gcagacgacg ccacggcaga cgtggacgtg gtggtcgtcg ccgaggcgct
      361 caagccggag gaccgcgcga tgccgcgcgg gcgtccgacg ctgctggtgc tcaacaaggc
      421 ggatctggcc ggtttcggtg ccgggggacc gatcgcggtg gccgaccggc gggccgccga
      481 gatcagggcg ctgaccggcg tgccgacggt gtcgatggtg gggctgctgg ctgtcgcggc
      541 gatggacgac gaactggtcg ccgcgctgca gacgttgacg gccgagccgg ccgacctgac
      601 ctcgaccgac agatttctgt cagccgggca cagcctggcc cgtgacgtgc gcgcgcgact
      661 gctggacacc ctcgacctct tcggcatcgc gcactgcgtg ctggcgctgc ggcagggcac
      721 ggatggcgcg gcgctacccg tcgtgctgcg tcggctcagc cggatcgacc ctgtggtggc
      781 acggatcgcc gcgacgggcg cggaggtgcg ctatcggcgg gtgcgctcgg cgctggtcga
      841 actgcgcacg atggcggcgc gcggcacgcc ggccgtaacc cagttcctgt ccggcgacga
      901 cgccgtcctc gccgcgatgg ccgccgctgt cgacgtggtg caggcggagg gcttgacggt
      961 cgacgccgag gaccagccgt cggcgcatct ccggcgcgcg ctgcactggc accgctacag
     1021 ccgtggccct gtgaacccca ttcaccgcga ctgcggagcc gacatctgtc gcggctcgct
     1081 gcggctgctg cgacgtacgg ggccccggtg accgacgtcg accaggcggg ggatccgatc
     1141 ggtgcggtgg atgcactcgt ggcaggggtg gatccggggc tgagctcacc gggtgtggaa
     1201 caccgcgacg tcgtgctcgt caccgggccg tggttggcgg gtgcgaccgg tctgatcacc
     1261 gcattgcggg atcggctgcc cgacgtcgag ttcgtcgaat ccgaggagat caggtcggcc
     1321 gacgcgcccg ctgcggtggt gttcgtcgtc tccgcggtag ctccgatcgc cgaatccgac
     1381 tgtgccctaa ttgatctcgc tgctcagcac acagatctgg tgatcggtgc ggtgtcgaag
     1441 atcgatgtac accgcgagtg gcgcgacgtg ctggccgagg atcggtcccg gctcgccgcg
     1501 catgcggggc gatatcggga tgtgccgtgg gtgggtgtgg cggcggcgcc cgatctcggc
     1561 gaaccgaacg tggaggagat cgtcgagctg ttgcggtcac ctctcgacga tgcggacgtg
     1621 aaaaggcgaa acaggttgcg ggcgtgggag tcccggttgc agaccgtcat cgggcgtcac
     1681 gacaaggacg gcgcgggcgg cgaccggcag gcacgggtga cggctcttcg ggagcggcgg
     1741 gacgaggtgc tgcgtaatcg cagggtgtcc aaatcggagc gcaccatcgc gttgcgcagc
     1801 cagatccaac aggcgcgtgt gcaactggcg tacttcgccc gaaaccgctg cacgtctgtg
     1861 cgttcggagc tgcaggacga cgcgtcggcg atgacccgtc gccggttgcc ggagtttgag
     1921 gcctacgtcg gcacccgcgt cagcgaggtg gtcagcgagg tcgaagccgg tatcacccgg
     1981 cacctcggcg acgtcgcgac cgaactcgaa ctgaccgcgc cggtgccgcc cccgccgccg
     2041 acgccgcccg cggtgtcccc gccgccgctg aagtcccgtc gcctggaaac acggctgatg
     2101 atggtgctcg gtgccggctt cggcctcggc gtcgcgttgg gcatcagcag gctgttcgcc
     2161 ggccttgcgc ccggcctgac gatcgccggg ctggtggccg gtggcgtggt cggtctgctg
     2221 ctgacggtgt gggtggtcgg catgcgggga gtgctcagcg accggtcggt gctggaccgc
     2281 tggatcaccg agttgaccgg cacgcttcag tccgcggtcg aggagcgggt cgccacccgg
     2341 gtactagccg ccgaagccac actgaccgcc gaggtggccc gccgcgacga agctgacgcc
     2401 gaggcggccg ccggtcaggt cgacgagatc gatgccgaac tacgcgagca cgcgatcgcc
     2461 accgcacggg ccgccgcgct gcgggaccgg cggctgccgc cactgcagaa ggctctggag
     2521 acggtgcgcg cgcagttggc gggcggaccg caggactgac tcaacagcga tcgagctggg
     2581 ctttctgaat cgttcctgtg agtgatcgga catagatccg gttaaccacg ggtatgtcac
     2641 ccgcgttaac ctctataaga gggtgataag caccagcggc gctttcgacc gcgtcctggc
     2701 ggaaaccgtt acgcaggaga ttttgatgac ctcagcgacc attcccggtc tggacaccgc
     2761 accgacgaaa cacgcaggac ttcttggctg ggtccaggag gtcgcggagc tcacgcagcc
     2821 cgatcgggtg gtgttcgccg acggctcaga cgaggagaac gcccgcctgt gcgagcaact
     2881 ggaggcggca ggcaccttca agaagctgga cgagacgaag aagcccaatt cgtacctggc
     2941 gctgtctgac ccatctgatg tggcgcgggt ggaatcgcgc accttcatct gttcggaacg
     3001 cgagatcgac gccggtccca ccaacaattg gatggacccc gccgagatgc ggggcatcat
     3061 gaccgatctg taccgcggct gtatgcgcgg ccgcaccatg tatgtggtgc cgttctgcat
     3121 gggcccgctc ggcgccgagg atcccaagct cggtgtcgag atcaccgact cggagtacgt
     3181 cgtcgtgtcg atgcggacga tgacccggat gggcaaggcc gcactggaga agatgggcga
     3241 agacggcttc ttcgtgaagg cgttgcactc gatcggcgcc ccgct
//
LOCUS       DASYLX010000717         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_587966,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000717 DASYLX010000000
VERSION     DASYLX010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587966"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGP27_16870"
     CDS             <1..369
                     /locus_tag="VGP27_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7853006.1"
                     /translation="GYDYGTPAGVPGGGYGGGYGQPDYPAGGATITLQLDDGSGRTYQ
                     LREGANVIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLADLNSTNGTTVNNAPVQEW
                     QLADGDVIRLGHSEIIVRVH"
     gene            482..949
                     /locus_tag="VGP27_16875"
     CDS             482..949
                     /locus_tag="VGP27_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7853007.1"
                     /translation="MQGLVLQLTRVGFLLLLWLFIWSVLRILRTDIYAPTGAVMVRRG
                     LALRGSLLPSRQKRRIARTLVVTDGALAGTRITLGSQPVLIGRADDSTLVLTDDYAST
                     RHARLSQRGSEWYVEDLGSTNGTYLDRAKVTTAVRVPLGTPVRIGKTAIELRP"
     gene            1025..>2323
                     /locus_tag="VGP27_16880"
     CDS             1025..>2323
                     /locus_tag="VGP27_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEV7853008.1"
                     /translation="MALVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGE
                     VASQLVIAALAHLDDDEPGGDLLSKLDSAVRDGNSAIAAHVEADPELEGMGTTLTAIL
                     FDGNRLGLVHIGDSRGYMLRDGELAQITKDDTFVQTLVDEGRITAEEAHSHPQRSLIM
                     RALTGHEVEPTLIMREARAGDRYLLCSDGLSDPVSHDTILDALQLEDVAESADRLIEL
                     ALRGGGPDNVTVVVADVIDYDYGQTQPILAGAVSGDDDQAAPPDTAAGRASAFNPRRN
                     EAKRVLPQPEEIPRPPRSRRKIVIAVAVLVLILLAGLYVGREILRSNYYVTQHDGTVS
                     IMRGVQGSFLGYQLQEPYLLGCLNERNDLSLISAGQSRTDLNCRMLNVNDMRPSERAQ
                     IIAGLPSGSLDNAIGQIEELARSSVLPTCPTRTPPPPTPTV"
BASE COUNT          405 a          809 c          750 g          359 t
ORIGIN      
        1 ggatatgact acggcactcc cgcaggcgta ccgggcggcg gctacggcgg cggctacggc
       61 cagcccgact acccggcagg cggcgccacc atcacccttc agctcgacga cggcagcggc
      121 cgcacctatc agttgcgcga aggcgccaac gtgatcggcc gcggccagga cgcccagttc
      181 cggctccccg acaccggggt gtcccgtcgg catctggaga tccggtggga tggccaggtc
      241 gcgctgctgg ccgacctcaa ctccaccaac ggcaccaccg tgaacaacgc tccggtgcag
      301 gagtggcagt tggccgacgg cgacgtgatc cggctgggac actccgagat catcgtccgc
      361 gtccactgag ggtctcggcc gcgggtcaca gcacgggtac aagctgtctg cttcttactc
      421 acgctgatgg ccgtccaagt atcgtgacgt tgccgaaggg tggggacgga gaggacgtcg
      481 gatgcaggga ttagtgctgc agctgacgcg cgtcggattc cttctccttc tctggctctt
      541 catctggtcg gtgttgcgga ttctgcgcac cgacatctac gcgcctaccg gagcggtcat
      601 ggtgcggcgc gggctggcgc tgcgcggttc gctgctgccg tcccgacaga aacgccgcat
      661 cgcccgaaca ctggtagtga ccgacggcgc gttggccggt acccgcatca ctttgggcag
      721 ccagccggtg ctgatcggtc gcgccgacga ctcgaccctg gtgcttaccg acgattacgc
      781 gtccactcgg cacgcccggc tgtcgcagcg gggctcggaa tggtatgtgg aggacctagg
      841 atcgaccaac ggcacatacc tcgacagggc gaaggtgact acggcggtac gggttccatt
      901 aggcacgccg gttcgaatcg gcaaaacggc aatcgagctg cggccgtgac gccgcgcacg
      961 cgaggagcgc aattgacgcc gcgcacgcga ggagcacaat gacgccgcaa acgcgaggag
     1021 cacgatggcg ctagtgcttc gatacgccgc gcgcagcgac cgcggcctgg tccgagccaa
     1081 caacgaggac tccgtgtacg ccggcgcgcg cctgctggca ctggcagacg gcatgggcgg
     1141 tcacgcggca ggcgaggtgg cgtcccaact ggtgatcgcc gcgttggcgc atctcgacga
     1201 cgacgagccc ggtggcgatc tgctctccaa gctcgactcc gcggtcaggg atggcaactc
     1261 ggccatcgcg gcgcacgtcg aggccgaccc cgaactcgag ggcatgggca ccacgctcac
     1321 cgcaatcctc ttcgacggca acaggcttgg gctcgtgcac atcggcgact cccgcggcta
     1381 catgttgcgc gacggggaac tcgcacagat caccaaagac gacaccttcg ttcagacact
     1441 ggtcgacgag ggccgcatca ccgccgagga ggcgcacagt cacccccagc gctcgctgat
     1501 catgcgggcg ctcaccggac acgaggtcga gcccacgctg atcatgcggg aagcccgtgc
     1561 cggcgaccgc tacctgttgt gctccgacgg gctgtccgac cccgtcagcc atgacacgat
     1621 cctcgacgcc ttgcaactcg aggatgtggc cgaaagtgct gacaggctca tcgaactcgc
     1681 actgcgcggt ggcggtccgg acaacgtcac ggtggtcgtc gccgacgtca tcgactacga
     1741 ctacgggcag acccaaccga tcctcgccgg cgcggtgtcc ggggacgacg atcaagccgc
     1801 tccacccgac accgcggcgg ggcgcgcgtc ggctttcaac ccgcggcgca acgaggccaa
     1861 gcgcgttctg ccgcaacccg aagagatccc gcgcccgccg cgttcgcgac gcaagatcgt
     1921 catcgcggtg gcggtgctgg tcctcatcct gctggccggg ctctacgtcg gcagggaaat
     1981 cctccgcagc aattactacg tcacccagca cgacggcacg gtgtcgatca tgcgcggtgt
     2041 tcagggttcc tttctcggct atcagctgca ggagccgtat ctcctcggtt gcctgaacga
     2101 acggaatgac ctgtcgctca tcagcgctgg gcaatcccgc accgacctga actgccggat
     2161 gctcaacgtc aacgacatgc ggccgtcgga acgcgctcag atcatcgccg gcctgccgtc
     2221 gggcagcctc gacaacgcca tcggtcagat cgaggagctc gcccgcagct cggtcctgcc
     2281 gacctgcccc acgcgcaccc cgcccccgcc gactccgacc gtc
//
LOCUS       DASYLX010000718         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3549305,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000718 DASYLX010000000
VERSION     DASYLX010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549305"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="trpD"
                     /locus_tag="VGP27_16885"
     CDS             <1..805
                     /gene="trpD"
                     /locus_tag="VGP27_16885"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV7853009.1"
                     /translation="DVVGTGGDGANTVNLSTMAAIVVAAAGVPVVKHGNRAASSRSGG
                     ADTLEALGVRIDLGPEEVARSVAEVGIGFCFAQTFHPSYKYAGAARREIGVPTVFNLL
                     GPLTNPGSPRAGLIGCAWGDLAEVMAGVFATRRCSVLVVHGDDGLDELTTTTTSTIWR
                     VQAGTVEKLSFDPAGFGFARADISELVGGDAEANAAEAREVLAGAKGPVRDAVILNAA
                     GAMVAHAGLTSGAEWLSAWESGLNRAVEAIDTGAAEQLLARWVRFTQQV"
     gene            complement(743..2530)
                     /locus_tag="VGP27_16890"
                     /pseudo
     CDS             complement(743..2530)
                     /locus_tag="VGP27_16890"
                     /inference="COORDINATES: protein motif:HMM:NF005903.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
     gene            2634..2870
                     /locus_tag="VGP27_16895"
     CDS             2634..2870
                     /locus_tag="VGP27_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853010.1"
                     /translation="MDEADKQGTDTGAVTIDCDDCAVRGPGCNDCVVSVLLGVPDTLL
                     VDERAALEVLAEAGMAPRLRLVPIHRPGSSGVVA"
     gene            3073..>3464
                     /locus_tag="VGP27_16900"
     CDS             3073..>3464
                     /locus_tag="VGP27_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEV7853011.1"
                     /translation="MICAIASLMLVASVVAGNVHADPADDALAKLNELSRQAERTTEA
                     MHSAQLDLNNKLAAQQEADAKHTADQAALDKATADLATFQSAVDKFAAAQYMGGRTSG
                     MDAMLTADSPQRLIDQLSVQRVMASEMSA"
BASE COUNT          559 a         1148 c         1258 g          499 t
ORIGIN      
        1 cgacgtggtg ggcaccggcg gcgacggtgc gaacacggtc aacttgtcca cgatggcagc
       61 catcgtggtg gccgccgcgg gggtgccggt ggtcaagcac ggcaaccggg ccgcgtcgtc
      121 gcgcagcggc ggcgccgaca cgctggaagc cctcggggtg cgcatcgacc tcggtcccga
      181 agaggtcgcc cgcagcgttg ccgaggtcgg gatcggcttc tgcttcgcgc agaccttcca
      241 cccgtcctac aagtacgcgg gcgccgcccg gcgcgaaatc ggcgtgccca ccgtgttcaa
      301 tctgcttggg ccgctgacca atccaggttc gccgcgcgcg ggactgatcg gttgcgcatg
      361 gggcgatctt gccgaggtga tggcgggagt gttcgcgact cggcggtgca gcgttctggt
      421 ggtgcacggc gacgacgggc tcgacgaact caccaccacg acgacgagca caatctggcg
      481 ggtacaggcg ggcacggtcg agaagctgtc gttcgatccg gcgggattcg ggttcgcgcg
      541 tgccgacata tccgaattgg tgggcggcga cgccgaagcc aatgccgccg aggcacgcga
      601 ggtgctggcc ggcgccaagg gccccgtccg cgacgcggtg atcctcaacg ccgcgggcgc
      661 gatggtggcc cacgccgggt taaccagtgg cgccgaatgg ctgtcggcct gggagtcggg
      721 gctgaaccgg gccgttgagg cgatcgacac cggggcggcc gaacagctgc tcgcgcgttg
      781 ggtgcggttc actcaacagg tgtgacgagt cgagggtctg ttcggcggcc agccgcgccg
      841 acctcgccga ggcggcccat gtcgcccacc gtcccgcgca ccccaccgac gacgcccagc
      901 cgggctcggc ccgcacgatc cgaacaccgg gctgggcgag ccagcgcatg atcagcgcga
      961 cctcttccac cagcgcgcca cccagcgggg cgggctctgg caggatcgcc tgtgccgcag
     1021 cacttatcgc gtcgacgatg ggcatcggcg gaacgccgcg aggtgcgacg cctgcggacg
     1081 ccagctgtcc gcaccgaacc accgccaggt gccatccgcc gctgccgtcg gggcgtgcgg
     1141 cgacgagttc ggtgaccgcc gcgagtgcgc gcagccgctg accgcgccac agcacatcaa
     1201 tagcggcggc cgcgtgatcg cgcagccgtg cggcggtctc gtagcggtgg tgcccggcga
     1261 gttcgccgat gtgggactgc accgccgaca gcgcggaatc ctccagcccg tcgatcagcg
     1321 ccgccgcgcg ccggggtgcc tccgcgtatt ccgcggcggg gacaccgcga cgcgccgggc
     1381 acggcgacaa ctcgcgttcg gggcaggtgg ggccgtggtg cgcggagcgc ccgatccttc
     1441 tggcgcaggt gcgcagcccg gtgaaacgag ccagcaggtc ggccgtctcg acggcgtcgg
     1501 cgcgcgcccg aaacggccca acggcgctgt cgcccttcgg ccttcgcacg atcgacagcc
     1561 gcgggaacgc ctcatcggtc agcacgatcc accaccaccg ctgcgggaac ttcgaccggc
     1621 ggttgtacgg cggcgaatgc gcggcgagca gccgcagctc gcgcacgccc gcctccaggt
     1681 cgtgtgcgca ctcgacgtgg tccacagcgg tggccagcgc gaccatctcc ttcatgcgcg
     1741 gccgcgagtc ggagccgttg aagtactggc cggcccgcct gcgcagatcg accgccgtgc
     1801 cgacatagag cacctcgttg gccgggccgc ggaacaggta gacgccgggc cggtggggca
     1861 ggttcgtggc gagcactcgc ttgcggcgct gcgcctgtgt cacgtcgggc aggtaggagc
     1921 gcagttcggt gtaggtgtgc acgccctgat tgccgacccg ctcgatgagc gcgtgaagca
     1981 cgtcgacggt ggctcgcgcg tcgtcgagcg cacggtgtgt cggtgtggtg gcggcaccga
     2041 acaaccgggc cagtgccgag agccgaacgc tgggggcttc gtcgcggctg agcacccgcc
     2101 gggccaaccg cacggtgcag agcaccggcg ggcgcggcca ggcgatctgg caccgctccg
     2161 cggcggcccg caggaagccg atgtcgaagc ccgcgttgtg ggcgaccagg accgcgccac
     2221 gggcgaactc gaggaacgcg ggcagcaccg agtcgatctt gggcgcgtcg cacaccatgg
     2281 ccgaggtgat gccggtgagc gcgacgatct gcggtggaat gctgcggccg gggtcgatca
     2341 gcgtggccag ctcgcccaac accgccccgc cgcgcacctt cacggcgccg atctcggtga
     2401 tggcgtcgac gttgtggcca tcgccggtgg cgcggccgcc cgtcgtctcg aggtcgacga
     2461 cgacgaaggt ggtgtcccgc agcgagacgt cggccagcgc atcgacgtcg gcgaagctga
     2521 gctggcccat gcgctgaagg taggcactgc taccgacagc gttgaagtgc cacgcccggg
     2581 atgtcggtgg tgcccgttac cgtgcgacta actgtagatc gagaggacgg cagatggacg
     2641 aggccgataa gcagggcacg gatacgggag cggtcaccat cgattgcgat gactgtgccg
     2701 tgcgtggccc cggctgcaac gactgcgtcg tgagtgtgct gctcggcgtg ccggacaccc
     2761 tgttggtaga cgagcgcgcg gcgctcgagg tactcgcgga ggccgggatg gctccgcggc
     2821 tgcgactggt gccgattcac cggccgggca gttcgggagt cgtcgcctga cgacacgcct
     2881 ggcaatcgat cgcgtccgaa aaactctgag agactgttaa atttgcgtct cctgtttgga
     2941 caagggtgat gccgtttcgt aacctgtctg agacctagga cagttcgggg cggctcgcca
     3001 acggcagttt gaggacacaa aaccttgacg ctcgacctaa cgcagcggat cacgcgcgga
     3061 gttaagcgac caatcatatg tgcgatagcg agcttgatgc tggtcgcaag tgttgtcgca
     3121 ggcaacgtgc acgccgaccc cgccgatgat gcgctggcaa aactgaatga actgtcgcgg
     3181 caggccgaac ggaccaccga ggcgatgcat tcggcacagc tggacctcaa caacaagctg
     3241 gccgcgcagc aggaagccga cgcgaagcac accgccgacc aggcggcgct cgacaaggcc
     3301 acggcggatt tggcaacgtt ccagtcagcg gtcgacaagt ttgccgcggc ccaatacatg
     3361 ggcggtagaa ccagcggcat ggacgcgatg ctgacggccg actcaccgca gcggctcatc
     3421 gaccaactct cggtgcagcg ggtgatggcc tcggagatgt cggc
//
LOCUS       DASYLX010000719         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_477846,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000719 DASYLX010000000
VERSION     DASYLX010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477846"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGP27_16905"
     CDS             <1..400
                     /locus_tag="VGP27_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV7853012.1"
                     /translation="DAFRARGAKLVFWDCYRPHAVQAELFDAVPDQNWVARPGTSARS
                     HESGRSVDVTLADGHYGWPIDMGTGFDDFSAKAKAFATDGLTAEQQANRKWLRDGMSA
                     GGLSVYAGEWWHFDGPDADVGQPILDVPLS"
     gene            complement(511..1731)
                     /locus_tag="VGP27_16910"
     CDS             complement(511..1731)
                     /locus_tag="VGP27_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7853013.1"
                     /translation="MTHLDSVFRREWGSTVAALARWSGDLSVAEDAVQEAFAEAMRAW
                     PRDGVPDNPSGWIMTVARNRALDRLRRESARPGKELAAVVDDIVARTDRAGIPHPVRD
                     DELRMMFTCAHPALDRASQLALTLRLISGLTVAEIARALLQSEAAVGQRITRAKNKIR
                     HANIPLRVPPAELLPERTPHVLSCIYSVFTEGYWSTAGPSAIRDELCDEGVRLAGELC
                     KLAPATRDTHALAALVLLHDSRRRTRVTTDGALVPLDEQDRSKWDRGRIAAGLQHLRL
                     AAGAEGPYLPQAVITAIHATAPTWDQTDWATICAAYDRLLAITESPVVRANRALAIGF
                     RDGFPAGLAALDQVADDPRLARSSTAASIRADLLRRAGRTDDARFWYEKAVSLAGSDP
                     ARAFLTRRITECSR"
     gene            complement(1738..>2253)
                     /locus_tag="VGP27_16915"
     CDS             complement(1738..>2253)
                     /locus_tag="VGP27_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7853014.1"
                     /translation="TDGPFAEGAEVAGGYYVFEADNLDEALALAGDIPAAKYGAVEVW
                     PMVHFSPARVPLTSDSWLALLLEPAGNVVEPGGQDWDEGIKAHAEFGKIAGDRAVGGA
                     PLHPPTTATTVRVRDGEVLLTDGPYAEGAEVANGFYYINAADRDEAIKVASMIPATTV
                     ELRQLAGVSGL"
BASE COUNT          360 a          794 c          756 g          343 t
ORIGIN      
        1 cgacgcattc cgtgcccgcg gggcgaagct ggtgttctgg gattgctatc gcccgcacgc
       61 ggtgcaggcc gagttgttcg acgccgtgcc ggaccagaac tgggtggcgc gccccgggac
      121 gtcggcgcgc agccacgagt caggccggtc ggtcgacgtg acgctggccg acgggcacta
      181 cgggtggccg atcgacatgg gcactggatt cgacgacttc tcagctaagg caaaggcttt
      241 cgccactgac ggcctcactg ccgagcagca ggccaaccgg aagtggctgc gcgacgggat
      301 gagcgcaggc gggctgtcgg tgtacgcggg ggagtggtgg cacttcgacg gcccggacgc
      361 cgacgtcggc cagcccatcc ttgacgtccc gctctcctag cacctctcct agcaccgcga
      421 cggtgcgtgt cagcacacga cacgccgcca agattcctgc gactgcgcac actcgcggca
      481 cggtgagcgt gcagggtcga cgggaagccg ctatctggag cactcggtga tgcggcgggt
      541 caggaaggcg cgcgccgggt cggagccggc aagcgacacc gccttctcgt accagaagcg
      601 cgcatcgtcg gtgcggcccg cccgtcgcag aagatcggca cggatggagg ctgcggtgct
      661 cgaccgcgcc agccgcgggt cgtcggccac ctggtccagg gcggccaggc cggcagggaa
      721 gccgtctcgg aacccgatcg ccagcgcgcg gttggcgcgc accaccggtg actcggtgat
      781 cgccagcagc cggtcgtagg ccgcacagat cgtcgcccag tccgtctgat cccaggtggg
      841 cgccgtagcg tgaatcgcgg tgatcaccgc ctggggcaga tacggcccct ccgcacccgc
      901 ggccagccgc aggtgctgca gtccggcggc gatccgcccg cgatcccact tgctgcggtc
      961 ctgctcatcc aacggaacca gcgcaccgtc ggtagtcaca cgtgtgcgcc gcctcgaatc
     1021 atgcagcagg acaagggcgg ccagcgcatg ggtgtcacgg gtcgcgggcg ccagcttgca
     1081 cagctcaccc gcgagccgga ctccctcgtc gcacagctcg tcgcggatcg ccgagggccc
     1141 tgcggtcgac cagtacccct cggtgaacac cgagtagatg cagctcaaca catgcggggt
     1201 gcgctccggc agcagctcgg cgggcggaac ccgcagcgga atgttcgcgt gccgaatctt
     1261 gtttttggcc cgagtgattc gctgtcctac cgcagcttcg gactgcagca gcgccctcgc
     1321 gatctcggca actgtcaggc cggagatcag ccgcagggtc agcgccagct gagaggcccg
     1381 gtccagcgcc gggtgcgcac aggtgaacat cattcgcagc tcgtcatcgc gaaccggatg
     1441 cggaatgcct gcgcgatcgg tgcgggccac gatgtcgtcc accactgcgg cgagttcctt
     1501 tccgggccgt gcggactcgc gccgtagccg gtccaacgcg cgattacgtg cgaccgtcat
     1561 gatccatccg ctgggattgt ccgggacgcc gtcgcgaggc catgcacgca ttgcttccgc
     1621 gaaagcctcc tgcacagcgt cctcggcgac ggaaaggtca ccagaccagc gcgccagcgc
     1681 cgccacagtg gaaccccatt cgcgccgaaa gacgctgtcc aggtgtgtca tccagggtta
     1741 aagtccggag accccggcca gctgtcgcag ctcgacggtc gtcgccggaa tcatcgacgc
     1801 caccttgatc gcctcgtcgc ggtcggctgc gttgatgtag tagaacccat tggccacctc
     1861 tgcgccttcg gcatacggcc cgtcggtcaa aagcacctcg ccgtcgcgca cccgcaccgt
     1921 cgtcgcggtc gtcggcgggt gaagcggcgc gccgcccacc gcgcgatcgc cggcgatctt
     1981 gccgaattcg gcatgcgcct tgatgccctc gtcccagtcc tgaccgcccg gctcgaccac
     2041 gttgccggcc ggttccagca gcagcgcgag ccagctgtcg ctcgtcagcg gaacacgtgc
     2101 gggcgagaaa tgcaccatcg gccacacctc gacggcaccg tacttcgcgg cgggtatgtc
     2161 accggccagc gccaacgcct cgtcgaggtt gtcggcctcg aaaacgtagt agccgccggc
     2221 cacttcggcg ccctccgcga acggaccgtc ggt
//
LOCUS       DASYLX010000720         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_368081,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000720 DASYLX010000000
VERSION     DASYLX010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368081"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VGP27_16920"
     CDS             <1..175
                     /locus_tag="VGP27_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV7853015.1"
                     /translation="DAEELTRVLGELLDSPLELRRLGVNGRQRALDVFSWESVAAQTV
                     AVYEMAMARVAQC"
     gene            169..900
                     /locus_tag="VGP27_16925"
     CDS             169..900
                     /locus_tag="VGP27_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853016.1"
                     /translation="MLTVDYDRLGVGAGTKVIDVGCGAGRHSFEAFRRGADVVAFDQN
                     ASDLNDVDEILQAMQEQGQAPASAKAEAVKGDALDLPYGDGTFDCVIASEILEHVPED
                     DRVISELVRILKPGGALAITVPRWLPERVCWALSDEYHANEGGHIRIYRADALGDKVV
                     AHGLKMTHSHHAHALHSPFWWLKCAVGTSKSDHPAVAAYHKLLVWDMMSRPWLTRTAE
                     SLLNPLIGKSVALYFRKPEVPVASD"
     gene            920..1966
                     /locus_tag="VGP27_16930"
     CDS             920..1966
                     /locus_tag="VGP27_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEV7853017.1"
                     /translation="MPGVLTPAQCRQTAQSIANTQESSGAIPWSVGGHTDPWDHVENA
                     MALTAASLLEPARAAFDWCRANQRADGSWPIQLRNGIVEDANSDSNFCAYIATGVWHH
                     VLSTGDRRFAEDMWPVVVTAVDFVLDLQRPSGEIVWARSESGPEPDALLTGCASIYHS
                     LRCALALADYFDDPQPEWEVAVGQLGHAIAHHSDLFVCKDRWSMEWYYPVLGGGLRGA
                     AARIRIDERWDDFVVPGLGIRCVDDRPWVTGAETCELVLALDAIGQSARAREQFAAMH
                     HLREEDGSYWTGLVFADGKRWPEERTTWTGGAMILAADALSRTTAANGMFRGADLPRG
                     LVGEFDCECATSGR"
     gene            complement(1971..2642)
                     /locus_tag="VGP27_16935"
     CDS             complement(1971..2642)
                     /locus_tag="VGP27_16935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853018.1"
                     /translation="MSNTDTALPAPAARLFALADQVTGFMPADEGRRLYDTAVRYLGD
                     GIGVEIGTYCGKSTLMLGAAAQQSGGVLYTVDHHHGSEEHQPGWEYHDTSLVDPVSGR
                     FDTLPSLRRALDSADLDEHVVTIVGKSVIVARGWRTPLRFLFIDGGHTEEAAQRDFDG
                     WARWVDVGGALVIHDVFPDPDDGGQAPFHIYQRALATGAFREVSATGSMRVLERVWGT
                     PGEPL"
BASE COUNT          443 a          891 c          967 g          459 t
ORIGIN      
        1 cgacgccgaa gaactgacgc gggttctcgg tgaactgctc gactcgccgc tggagctgcg
       61 ccgtctcggc gtcaacggcc gccagcgcgc gctcgacgtg ttcagctggg aatccgttgc
      121 ggcacaaacg gttgcggtgt acgagatggc gatggcgagg gtcgcacagt gctgacggtc
      181 gactacgacc ggttgggggt cggcgcgggc acgaaggtga tcgacgtcgg ttgtggtgcg
      241 ggtcgccatt cgttcgaggc gttccggcgc ggagcggacg tggtcgcctt cgatcagaac
      301 gcgtccgatc tcaacgacgt cgacgagatc ctgcaggcga tgcaggagca gggccaagcg
      361 cccgcgtcgg ccaaggccga ggcggtcaag ggggacgcac tcgacctgcc ttatggcgac
      421 ggcacattcg actgcgtcat cgcttcggag atcctcgagc atgtccccga ggacgaccgg
      481 gtgatctctg aactggttcg cattctcaag ccgggcggcg cgctcgcgat caccgtgccg
      541 cggtggctgc cggaacgggt gtgctgggcg ctgtcggatg agtaccacgc caacgagggc
      601 gggcacatca ggatctaccg cgccgacgcg ctgggagaca aggtcgtcgc ccatggcctg
      661 aagatgaccc acagtcacca tgcgcacgcg ctgcactcac cgttctggtg gctgaaatgc
      721 gctgtcggaa cctcgaagtc ggatcatccc gcggtggcgg cctaccacaa gttgctggtg
      781 tgggacatga tgagccggcc ctggctgacg cgcaccgccg agtcgctgct gaacccgctg
      841 atcggcaaga gcgtggcgct gtacttccgc aagccggagg ttcccgttgc ttcggattga
      901 caagcctgac gttcccgcgg tgccgggcgt cctgacacct gcgcagtgca ggcagaccgc
      961 gcagtcgatc gcgaacaccc aggagtcctc cggcgccatc ccctggtccg tcggcgggca
     1021 caccgatccg tgggatcacg tcgagaacgc gatggcgctg acggccgcga gcctgctcga
     1081 acccgcgcgc gcagcgttcg attggtgccg ggccaaccaa cgggccgacg gatcgtggcc
     1141 gatccaactg cgcaatggca tcgtcgagga cgccaacagc gacagcaact tctgcgccta
     1201 catcgccacc ggagtctggc accacgtgtt gagcaccggc gaccggcgat tcgccgagga
     1261 catgtggccg gttgtcgtca cggccgtcga ctttgtcctc gacctgcagc ggccgagcgg
     1321 cgaaatcgtc tgggcccgta gtgaatccgg accggagccg gacgcgttgc tgaccggatg
     1381 cgcaagcatc taccacagcc tgcgctgcgc gctggcactg gcggattact tcgacgaccc
     1441 gcagcccgag tgggaggtcg ccgtcggcca actcggccat gcgatcgcgc accactccga
     1501 cttgttcgtc tgcaaggacc gctggtcgat ggagtggtac tacccggtac tcggcggtgg
     1561 cctgcgcggc gcggccgccc gtatccgcat cgacgaacgc tgggacgatt tcgtcgttcc
     1621 gggtctcgga atccgttgtg tggacgaccg gccgtgggtg actggcgccg aaacgtgcga
     1681 actggtcctg gctttggacg ccatcggcca gagcgcccgc gcccgcgagc agttcgcggc
     1741 catgcatcat ctgcgggagg aggacggctc ctactggacc gggctggtct tcgccgacgg
     1801 caagcgctgg cccgaggaac gcaccacgtg gacgggcggt gccatgatcc tcgccgcgga
     1861 tgcgttgtcg cgcacgaccg ccgccaacgg catgttccgc ggcgcggatc tgccgcgcgg
     1921 cctggtgggc gagttcgact gcgagtgcgc gaccagcggt cgctgaaaat tcagagcggc
     1981 tcgcccggtg tgccccagac gcgctccaac accctcatcg agccggtagc ggacacttcg
     2041 cggaaggcgc cggtggcgag tgcccgctgg tagatgtgga acggcgcctg gcctccgtcg
     2101 tcggggtcgg ggaagacgtc gtgaatgacc aacgcgccgc cgacgtcgac ccatctggcc
     2161 cagccgtcga agtcgcgttg tgcggcctcc tcggtgtgtc cgccgtcaat gaacagaaac
     2221 cgcaagggcg ttcgccagcc gcgggccacg atgaccgact tgccgacgat ggtgacgacg
     2281 tgctcgtcga ggtcggcgga gtctagtgcg cgacgcagcg acggcagcgt gtcgaatcgt
     2341 ccgctgaccg ggtcgaccag tgaggtgtcg tggtactccc aaccgggctg gtgctcttcg
     2401 gaaccgtgat ggtgatcgac ggtgtacagc acaccgccgc tctgttgcgc cgcggcgccc
     2461 agcatcagcg tcgacttgcc gcagtaggtt ccgatctcga cgccgatgcc gtcgccgaga
     2521 tagcggaccg cggtgtcgta gagcctgcga ccttcgtcgg cgggcatgaa gccggtgacc
     2581 tgatcggcga gtgcgaacag ccgtgcggcg ggcgcgggca gtgctgtgtc ggtgttgctc
     2641 atcgcgtcca acttaccgtt gctggtgaag ggtggttgta gtagcgtccg gacacgtgtc
     2701 cgagccggtc ctggagtcga cccggcgccg tctgaccgcc aagcaggccg acaccgtcga
//
LOCUS       DASYLX010000721         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_698914,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000721 DASYLX010000000
VERSION     DASYLX010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698914"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VGP27_16940"
     CDS             <1..745
                     /locus_tag="VGP27_16940"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV7853019.1"
                     /translation="QLRAMENARLERGLSPSPLLLENPGVEIIARYVPSRENTLLGGD
                     FYDFVQTPDRTVHVMIGDVAGHGPDEAALGVALRIAWRALTFAGLRGPDRMRELEQIL
                     CTERAGMGTFATVLSLAIPPEGSRFGAVRAGHPGMLVHGRGTVDWLEPPGGPALGLQS
                     SGWDYHQVEWPEATGLVLLTDGLFEGHSGRGNQRLGEDGLLELARKHAPLPGPEFVDA
                     LIGEAAQRAQAHGGLTDDIAVVRVQRTSR"
     gene            742..2370
                     /locus_tag="VGP27_16945"
     CDS             742..2370
                     /locus_tag="VGP27_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEV7853020.1"
                     /translation="MTKPNAGAPAGKPKPGRRLTVQGWQNLVLSAMGVVVLVGLVAGG
                     LLMHRTDSLSRELIDNIQPARVQAYAMQAALRDQETSVRGYVITGDKQFLDPYYTGLD
                     AERQAAQNVRERVGTRSDLMADLDEIEDAAAAWRMSYANPIIVDVSRGAPREASRVLT
                     DRGKIEFDRLRANFDRQNENLRTARTDGITELDHMRHWRDGLAIAMVVAFVVLAVVLA
                     LLVRAAVTRPLDALAAACRRITEGNFDERIVPRGPRDIRSIAAAVEDMRQRIVDELDA
                     SRSAKSTLDEQTEELRRSNAELEQFAYVASHDLQEPLRKVASFCQLLEKRYGDKLDER
                     GTEYIGFAVDGAKRMQLLINDLLTFSRVGRLNSTHIDTALDTALDTALANLSTAIEES
                     NADIVRPETRLPTVDGDPTLLAMLWQNLIGNAMKFHRDGLDPRIVIDARPPVGPEDRD
                     WLFSVTDNGIGIADDFAEKVFVIFQRLHGRDEYSGTGIGLAICKKIVEHHGGSIWVDT
                     SYSGGTRFKFTLPELSTAEPEPDILKPALEGTNQ"
     gene            2367..>2734
                     /locus_tag="VGP27_16950"
     CDS             2367..>2734
                     /locus_tag="VGP27_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7853021.1"
                     /translation="MTSAVRAIDVLLVEDDAGDELITREAFEHNKISNNLHVAHDGEE
                     GLDFLYKRGPYKDAPRPDLILLDLNLPKYDGRQLLEQIKNDNDLCHIPVVVLTTSSAE
                     EDILKSYKLHANAYVTKPVDL"
BASE COUNT          521 a          908 c          869 g          436 t
ORIGIN      
        1 tcaactgcgg gccatggaga acgcccggct cgagcgcggc ctttcgccgt cgcctctact
       61 gctcgagaac cccggtgtcg agatcatcgc gaggtacgtg cccagccggg agaacacgct
      121 gctcggcggc gacttctacg atttcgtcca gacgccggac cggacggtcc atgtgatgat
      181 cggcgacgtc gcaggtcacg gtcccgacga ggccgccctg ggcgtggcac tgcgtattgc
      241 ctggcgcgcg ctgacgttcg ccggccttcg tgggccggac cggatgcggg agctcgagca
      301 aatcctgtgt accgagcgcg cgggcatggg caccttcgcg acggtgctga gcctggccat
      361 tccgcccgaa gggtcccggt tcggcgcagt gcgcgcggga catcccggga tgctggtgca
      421 cggccgagga acagtggact ggctcgagcc gccaggcggc ccggcgctgg gactgcaatc
      481 cagcggatgg gactatcacc aggtggagtg gcccgaggcc acggggttgg tgctgctcac
      541 cgacggcctg ttcgagggcc actccgggcg gggcaaccaa cgcctcggtg aggacgggct
      601 gctcgagttg gcgcgcaagc atgccccgct gccagggccg gagttcgtgg atgccctcat
      661 cggtgaagcc gcacagcgcg cccaggcaca cggcgggctg accgacgaca tcgcggtggt
      721 ccgcgtgcag cgcacatctc gatgacgaag ccgaacgcgg gcgcgcccgc cggcaagccc
      781 aagcctgggc gcaggctgac cgtgcaggga tggcagaacc tggtgctgtc ggccatgggt
      841 gtggtggtgt tggtcggcct ggtggccgga gggctgctga tgcatcggac cgacagtctg
      901 tcgcgtgagc tgatagacaa catccagccg gcacgagtgc aggcctatgc gatgcaggcg
      961 gctcttcgcg atcaggaaac ctcggtgcgg ggctacgtca tcaccggcga caagcagttc
     1021 ctcgatccgt attacaccgg cctggacgcc gaacggcaag cggcacagaa cgtccgagag
     1081 cgagtcggta cacgatcaga tctgatggcg gacctcgatg agatcgagga tgccgcggcg
     1141 gcctggcgga tgtcctatgc aaaccccatc atcgtcgacg tctcccgcgg cgccccccgc
     1201 gaggccagtc gggtgctcac ggatcggggc aagatcgaat tcgaccgtct ccgagcgaat
     1261 ttcgatcgcc agaacgagaa tctgcgcacc gccagaactg acgggatcac cgagctcgac
     1321 cacatgaggc actggcgcga cggccttgcc atcgccatgg tcgtcgcgtt cgtcgtcttg
     1381 gcggtggtgc tggcgctgct ggtgcgcgcc gcggtcaccc gaccgctgga cgcccttgcc
     1441 gccgcgtgcc gccgcatcac cgagggaaat ttcgacgaac gcatcgtgcc ccgcggtcca
     1501 cgagacattc gttcgatcgc ggccgcggtt gaggacatgc ggcaacgcat cgtcgacgaa
     1561 ctcgacgcct cgaggtccgc caagtccacg ctcgacgaac agaccgaaga gttgcggcgg
     1621 tccaacgccg aactcgagca attcgcctat gtcgcctccc acgatttgca ggagccgttg
     1681 cgcaaggtcg cctcgttctg tcagctgctc gagaaacgct acggcgacaa gctcgacgaa
     1741 cggggaaccg aatacatcgg cttcgcggtt gacggcgcca aacgcatgca gctgctgatc
     1801 aacgatctgt tgacgttctc ccgcgtcggg cgtctcaact ccacccacat cgacaccgcg
     1861 ctggacaccg cgctggacac cgcgctggcc aacctgtcga ctgcgatcga ggaatcgaat
     1921 gccgacatcg tgcgccccga gactcggctt cccaccgtcg acggcgaccc gaccctcctg
     1981 gcgatgctgt ggcagaacct catcggcaac gcgatgaaat tccaccgcga cggactcgat
     2041 ccgcggatcg tcatcgacgc tcgacccccc gtcggccccg aggaccgcga ctggctgttc
     2101 agcgtcaccg acaacggcat cggtatcgcc gatgacttcg ccgagaaggt gttcgtcatc
     2161 tttcagcgtc tgcacggccg cgacgagtac agcggaacgg gcatcggtct tgcaatatgc
     2221 aagaagatcg tcgagcacca cggcggatcc atctgggtcg acacgtccta ctccggcggc
     2281 acacgcttca agttcaccct gcccgaattg tcgacagccg agcccgagcc cgacatactt
     2341 aagcccgctc tggaaggaac aaaccagtga cctcagctgt acgtgccatc gacgtcctgc
     2401 tcgtcgagga cgatgccggt gacgaactca tcacccggga agcattcgag cacaacaaga
     2461 tcagcaataa tctccacgtc gcccacgacg gcgaggaggg ccttgacttc ctctacaaac
     2521 ggggacccta caaggacgca ccgcggcccg acctgatcct gctcgacctg aacctaccga
     2581 agtacgacgg caggcaactg ctggagcaga tcaagaacga caacgatctg tgccacattc
     2641 cggtggtcgt gctgacgacg tcgtcggcag aagaggacat cctcaagagc tacaaactgc
     2701 acgccaacgc ctacgtcacc aagccggtcg atct
//
LOCUS       DASYLX010000722         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3220122,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000722 DASYLX010000000
VERSION     DASYLX010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220122"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGP27_16955"
     CDS             <1..381
                     /locus_tag="VGP27_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7853022.1"
                     /translation="IADRVLVMYAGQVVEDATLDEIFYEPKHPYTWGLLGSLARLDQP
                     RTTRLAQIPGAPPSLLSPPPGCRFAPRCSYVFDECIELPPLEPRTAGGHLDRCWLDAE
                     VKAQHGYRAREEQNGYRAREEKNG"
     gene            374..1363
                     /locus_tag="VGP27_16960"
     CDS             374..1363
                     /locus_tag="VGP27_16960"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEV7853023.1"
                     /translation="MGKSDVLLEVNDLVKHFPIRSGVVVDREVARVRAVDGVSFSLRE
                     GETLGLVGESGCGKSTLCRAILQLTTPTSGSVRFAGQELVGRSRRELRPLRRQMQMIF
                     QDPFASLNPRKRVGQIIGDPIELHGLARGAELKRRVQALLDRVGLNAEHYNRFPHEFS
                     GGQRQRIGIARALALRPKLIVADEPVSALDVSVQAQIINLLEDVQDEFGLSYLFVAHD
                     LGVVRHVSDRIAVMYLGKIVEDSGADELYDKPIHPYTNALLSAVPIPDPKRNSARERL
                     VLEGDVPSPINPPSGCRFHTRCPWATEICTSDEPALLDHRPQHFAACHHPQNV"
     gene            complement(1367..1771)
                     /locus_tag="VGP27_16965"
     CDS             complement(1367..1771)
                     /locus_tag="VGP27_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853024.1"
                     /translation="MSNNGPFGFDPDDFDRMFREAGEGLRDAVGRLFDMTGERAGLSV
                     LFDEFSRRQRHEPETTGETGDGAWAIYTVDDDGKAHVEEVYATELDALRANKNNTDPT
                     RKVRFLPYGIAVSVLDASPNVDEVSDAADDAE"
     gene            complement(1787..3640)
                     /locus_tag="VGP27_16970"
     CDS             complement(1787..3640)
                     /locus_tag="VGP27_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7853025.1"
                     /translation="MSDPLGLSVGTTNLVAARVGNQPVTRRSVLTLGQSGGTISGFVE
                     RVGDRVPLVTPDGTSYLAEQLLVEALDAMVELGGGPSSDVAIAVPAHWSPTTIWAFRS
                     ALVNSRVLHTSTPVRVVSDAVSALTALQANPGLTADGVVALIDFGGSGTSLTLANAAA
                     SFEPIGETVRYAEFSGDLIDQALLTHVVDNLADKVDPAGTTAVESLTKLREECRRAKE
                     RLSADTATQLVLDLSGYRADIRLTRDELQELIRRPLDGVVAALDELTRRNGTSPTDLA
                     ALAIVGGGAGIPFVTQRLSEHTQLPMVSPSQPALNAAVGAALFAAFGVDADAPTGANA
                     ATALDADAPTGAAAAATDVTVAGAVYEGPSSDAPGSASFRALAWSQDEGGEELVPYTG
                     ENPYLVTDSALRPAIEYLPTTGVIYKEPRRPWHRVAGLAIGIAAVIAIITVGGVAYAL
                     TSATESAKATEQSKVDPPKQPEPKQPQPEPPSPPKQESPPPPEPPPPSPEPPPPSAVT
                     ITSEAPPPPPPSPTWTPTTTVPTTTRTTTTTSPTTTTTSPTTTTTSPTTTTTTTTTST
                     TPSMTTSYIRVPFVPVPVPIQVPNQGPAYQEPPAYQPPAYQQPVYPQSPWG"
     gene            3777..>4504
                     /locus_tag="VGP27_16975"
     CDS             3777..>4504
                     /locus_tag="VGP27_16975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7853026.1"
                     /translation="MTSLWLADRANHPSPPSPLLEADRHADVVVVGAGITGLVTAVLL
                     ARAGKSVIVLEALSAGAGATGNTTAKISLLQGTKLSKIIAKHGPKRAEQYVEGNREGQ
                     EWLIGHCEAHGISVQREDAFTYAQSEDGVPSAKAELEACKAAGLAADWVDDAGVPFPF
                     HGGVRVADQAQFDPMPFLDSLIVELDERGGRLAQGIRVQKVSSEGDGLALQVRTTNAD
                     EFVVTAKQCVLATGIPILDRGGFFA"
BASE COUNT          719 a         1477 c         1522 g          786 t
ORIGIN      
        1 atcgccgacc gggtgttggt gatgtatgcg ggtcaggtcg tcgaggacgc gacgctcgac
       61 gaaatcttct acgagccgaa gcatccctac acctggggtc tgctcggctc actcgcccgg
      121 ctcgatcagc cgcgcaccac acggctggcg cagattccag gggcgccgcc gtcgctgctg
      181 tccccgccgc caggttgtcg cttcgccccc cgctgctcct acgtcttcga cgagtgcatc
      241 gagcttccgc ccctcgaacc acgcaccgcg ggcgggcacc tcgaccggtg ctggctcgac
      301 gcggaggtca aggcgcagca tggctaccgc gcacgcgagg agcaaaacgg ctaccgcgca
      361 cgcgaggaga aaaatgggta agagcgatgt tctgctggag gttaacgatc tcgtcaaaca
      421 cttcccgatc aggtcggggg tcgtcgtcga ccgcgaggtc gccagggtgc gagcagtcga
      481 cggcgtcagc ttcagtctgc gggaaggcga aacactgggc ctggtaggcg aatccggctg
      541 tggcaagtcg acgctgtgcc gggcgatcct gcaactgacg accccgacgt ccgggtcggt
      601 acggttcgcg ggccaggaac tggtcggccg ctcgcgccgc gagctgcgcc cgttgcggcg
      661 acagatgcag atgatcttcc aggatccgtt cgcgtccctc aacccgcgca agcgggtggg
      721 ccagatcatc ggcgacccga tcgaactgca cgggctggca cggggcgcgg agctcaaacg
      781 gcgcgtccag gccctgctcg accgcgtcgg cctgaacgcc gagcactaca accgcttccc
      841 gcacgagttc tccggcggtc agcgtcaacg catcggaatc gcccgcgcac ttgcgttgcg
      901 gcccaagctg atcgtcgccg acgaacccgt ctccgcactc gatgtctccg tgcaggcaca
      961 gatcatcaac ctgctcgagg atgtgcagga cgagttcggg ctgtcctacc tcttcgtcgc
     1021 ccacgacctc ggcgtcgtcc gtcacgtgtc cgaccggatc gcggtcatgt atctgggcaa
     1081 gatcgtcgag gactccggcg cagacgagct gtacgacaag ccgatccacc cgtacaccaa
     1141 tgcactgctg tccgccgtgc cgatccccga cccgaagcgg aactccgcac gggaacgcct
     1201 ggtgctcgag ggcgatgtgc cgagccctat caatccgccc agtggctgcc gcttccacac
     1261 gaggtgccca tgggcgaccg agatctgtac gtcagacgag ccggccctgc tcgaccaccg
     1321 accgcagcac ttcgcggcgt gtcatcatcc gcagaacgtc taaggactat tccgcgtcgt
     1381 ctgcggcgtc cgacacctca tcgacattcg gcgacgcgtc cagcacgctg accgcgatgc
     1441 cgtagggaag gaagcgcacc ttgcgcgtcg ggtcggtgtt gttcttgttc gcccgtagcg
     1501 cgtcgagttc ggtggcgtag acctcttcga cgtgggcctt gccgtcatcg tcgacggtgt
     1561 agatggccca cgccccgtcg cctgtttcgc cggtggtttc cggctcatgt cgctggcggc
     1621 gggagaactc gtcgaaaagg acgctcaacc cggcccgctc gccggtcatg tcgaagagtc
     1681 tgccgaccgc gtcgcgcagt ccctcgccgg cctcacggaa catccgatca aagtcgtcgg
     1741 ggtcgaaccc gaacggacca ttgttgctca tcaatccagt gtgccactaa ccccagggac
     1801 tctgcgggta caccggctgc tggtaggccg ggggctgata cgcagggggc tcctgatacg
     1861 ccgggccctg gttgggcacc tggatcggca ccggcaccgg cacgaacggg accctgatgt
     1921 agctcgtcgt catcgacggc gtggtgctgg tggtcgtcgt cgtggtcgtc gtcgtgggtg
     1981 atgtcgtcgt ggtggtcggt gacgtcgtcg tggtcgtcgg cgacgtcgtg gtggtcgttc
     2041 ttgtcgtggt gggcaccgtg gtggtgggcg tccacgtcgg cgacggcggg ggtggtggcg
     2101 gcgcttcgct cgtgatcgtc accgcactcg gcggcggtgg ctccgggctc ggtggcggcg
     2161 gctccggggg cggagggctc tcctgcttcg gcggcgacgg cggctcgggc tggggctgct
     2221 tcggttcggg ctgcttgggc ggatcgacct tcgactgctc ggtggccttc gcactttcgg
     2281 tggcgctggt cagcgcgtac gccacaccgc ccactgtgat gatggcgatg acggcggcga
     2341 tgccgatcgc aagcccggca acgcggtgcc acggacgtcg cggctccttg tagatcaccc
     2401 ctgtcgtcgg taggtactcg atcgccgggc gtagggcgga atccgttacc aggtaggggt
     2461 tttcaccggt gtaggggacg agctcctcgc cgccctcatc ctgcgaccac gccaacgcgc
     2521 gaaacgacgc cgagccgggc gcgtccgaac tcggcccttc gtagacggct ccggccactg
     2581 ttacatcggt ggcggcagcg gcggcacccg ttggggcgtc cgcgtcgagg gccgtcgccg
     2641 cgttggctcc ggtcggcgcg tcggcatcga ctccgaacgc cgcgaacagc gcggcaccga
     2701 cggccgcatt gagcgccggc tgtgaagggc tcaccatcgg aagctgcgtg tgctcggaaa
     2761 gccgctgggt gacaaacgga atgccggcgc cgccaccgac gatcgcgagc gcggcgagat
     2821 cagtcggcga cgtgccgttg cgtcttgtca gttcgtccag cgcggcgacg acgccgtcca
     2881 gaggccgccg gatcagttcc tggagttcgt ctcgggtgag ccggatgtcg gcgcggtaac
     2941 ccgaaagatc gagaaccagt tgagtggcgg tgtctgccga cagccgctcc ttggcgcgcc
     3001 tgcattcctc gcgcagcttg gtgagcgact cgaccgccgt ggttccggcg ggatcgacct
     3061 tgtcggcgag gttgtccacg acgtgggtca gcagcgcctg atcgatcaga tcaccggaga
     3121 actccgcata ccgcacggtt tcgccgatgg gctcgaagga cgccgcggcg ttggccagcg
     3181 tgagactggt gccgcttcca ccgaagtcga tcagcgccac cacgccatcc gcggtgagac
     3241 cgggatttgc ctgcagggcc gtcagtgcgg acaccgcgtc ggaaaccacc cgaaccggcg
     3301 tgctggtgtg caggaccctg ctgttgacga gtgcgctgcg aaacgcccat atcgtggtcg
     3361 gactccagtg cgcgggaacc gcgatcgcga cgtcggacga cggaccgcca cccaactcca
     3421 ccattgcgtc gagcgcttcg accaggagtt gttcggcgag atagctcgtt ccatcggggg
     3481 tcaccagcgg gacccggtcc ccgacacgct cgacgaagcc gcttatggtg ccgccggatt
     3541 gcccgagggt cagcaccgag cggcgcgtca caggttgatt gccgacacgc gccgcgacca
     3601 ggtttgttgt cccaaccgac aaccccaagg ggtcgctcat aacggcccac accatagccc
     3661 tgttgtgggc agatagcgct cagacactcc gtgcacagcc cgtttgaaca gcgttccgcc
     3721 accatgcttt tgagtgcttg cgttttatca ccgccacgta gggtactggc tgagacatga
     3781 cttcgttgtg gctggctgac cgggcaaacc atccatcacc gcccagtcct ttgctcgagg
     3841 ccgaccgtca cgccgacgtg gtggtggtcg gcgccggaat caccgggctc gtcactgccg
     3901 tgctgttggc cagggcgggc aagagcgtca ttgtgctcga agcgctttcg gcaggcgcgg
     3961 gcgccaccgg caacaccacc gcgaagatca gcctgctgca gggcaccaag ctgtcgaaga
     4021 tcatcgccaa gcacggcccg aaacgcgctg agcaatatgt cgaaggcaat cgcgagggcc
     4081 aggaatggct gatcgggcac tgcgaggcgc acggcatctc ggtgcagcgc gaagacgcct
     4141 tcacctacgc gcaatccgag gacggtgtgc cctcggcgaa ggctgaactc gaggcgtgca
     4201 aggccgccgg gttggcggcg gactgggtcg acgacgcggg cgtaccgttc cccttccacg
     4261 gcggcgtccg cgtcgccgac caagctcagt tcgatcccat gccgttcttg gacagcttga
     4321 tcgtcgaact cgatgagcgt ggcggacgcc tcgcgcaagg gattcgggtg caaaaggttt
     4381 caagtgaagg cgacgggttg gcgctgcagg tgcggacgac aaacgccgac gagttcgtcg
     4441 tcacggcgaa gcagtgtgtg ctcgccaccg gtattcccat tctggatcgc ggcgggttct
     4501 tcgc
//
LOCUS       DASYLX010000723         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2562127,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000723 DASYLX010000000
VERSION     DASYLX010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562127"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..499)
                     /locus_tag="VGP27_16980"
                     /pseudo
     CDS             complement(1..499)
                     /locus_tag="VGP27_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247422.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(499..1683)
                     /locus_tag="VGP27_16985"
     CDS             complement(499..1683)
                     /locus_tag="VGP27_16985"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7853027.1"
                     /translation="MSAAQRKESLDDWGPVPFEEPLAMLADSYSAADLNDIGVHILRS
                     SLVHSLRMRLRAEEWIRRHPEITDESIIAPIVVVGMMRSGTTLLQRLLAADSRFHCAY
                     GWEVVEVAPRLDYDFSAQPDPRVAISERREASSRELAPALFSIHPMYAREPEEEIVFL
                     SDAFLSHVPESGAHVPRYRSWIDEQDFAPAYIYLHRMLQFLQWQKRFADARSTTAGSG
                     RTAGRWVLKSPAHLGYLDTLRAHFPDLHIVHMHRDPKDTIASGASLNATLHAMHADEV
                     DRNRVGAEWLHRMGWTNDRAMGTRAMWIDETLRCTDIDFEDAVADLIGQVARVYDAIE
                     VPLTAEAESAMRHWLKERPREPARPPYAAADFGLSDAEIDERFAAYTSRFRSGATSSR
                     RN"
     gene            complement(1710..2162)
                     /locus_tag="VGP27_16990"
     CDS             complement(1710..2162)
                     /locus_tag="VGP27_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853028.1"
                     /translation="MRDDAREIENLVYTYAERLDAGDLDGVAALFAHGRICGMEDGPP
                     ETVFAGVARVRQMYEMATRLYEDGTPKTKHLTTNVALHVDEDAGTADGSAYYCVTQAT
                     PELPLQVIVTGRYRDTFHRVDGTWWFDTRIMYVDQVGDTSQHLKFSPP"
     gene            complement(2372..>2837)
                     /locus_tag="VGP27_16995"
     CDS             complement(2372..>2837)
                     /locus_tag="VGP27_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV7853029.1"
                     /translation="TATADPVTVTATVLKIVDGDTIDIRDDVRGRLRVRLLGIDTPET
                     KKPGYTVGCWGPEATEFAESTMLGQRVALVADPTQDKTDRYGRTLGYLVRQDGWDYSV
                     EAARAGAARSYIYGGVPVSRYDAIAGAEQQARTARRGLWGAPCNGDTASAKQ"
BASE COUNT          462 a          905 c          930 g          540 t
ORIGIN      
        1 gacggtgatg gtgaacgatc cggcgctgtc cacctccatg gtgcggccgt tgagcacagc
       61 aaccgtgccc atgtcggcat cccacagcgt gaagtagttc tccgtcatcc ggttcgcgcc
      121 cacccgaccg cggatctcgt agcgctcgtc ggcggagatg ggaatgacgc ggtagacgct
      181 gtcgggattg tcaatgcccc aacgagatcc gggaatggtg cgaccggcca ccgggtggcc
      241 gatccgggtg atgcagctga ccttcggccg cagcttgtcc tggtttggac gaccacaccg
      301 ccgcggagaa catcacctct gcgaaggcgt cgtcgaatcg ctcgcgcatc gcatcggagg
      361 ccttcgcccg gccgagccac gcctcgccga cgctgcggta cgccgccttg acggtcggat
      421 gatccatcag ttcgagagcg gcgagctcct gttcgtgctg tgactcggtg gctaccggat
      481 cgccggcgaa gtcggacatc agttcctcct cgaagaagtc gcgcctgagc ggaatcgaga
      541 ggtgtaggcg gcgaaccgct cgtcgatctc ggcgtcgctc agcccgaagt cggcggctgc
      601 ataaggcggg cgcgcaggct cgcgtggacg ttctttcagc cagtgccgca tcgccgattc
      661 ggcttcggcg gtgaggggca cttcgatggc gtcgtagacg cgagccacct gaccgatcag
      721 atcagcgaca gcgtcttcga aatcgatgtc cgtacaccgc agggtttcgt cgatccacat
      781 cgcgcgggtg cccatcgcgc ggtcattggt ccagcccatc cggtgcagcc attccgcgcc
      841 gacgcggttc cggtccacct cgtcggcgtg catcgcatgc agtgtcgcgt tgaggctggc
      901 gccggaggcg atggtgtcct tcgggtcgcg gtgcatgtgc acgatgtgca ggtcaggaaa
      961 gtgggcgcgc agtgtgtcca gatagccgag atgggcaggg gatttgagca cccaacggcc
     1021 tgcagttcgg ccgcttcccg cggttgtgct cctcgcgtcc gcgaaacgct tctgccactg
     1081 caagaactgc aacatccgat gtaggtagat gtacgcaggc gcgaagtcct gttcatcgat
     1141 ccaggaccgg tagcgcggca cgtgcgcgcc ggactcgggc acgtgcgaca ggaacgcgtc
     1201 ggagagaaag acgatctcct cttcgggttc gcgggcgtac atggggtgga tcgagaacaa
     1261 cgcgggagcc agctcgcgcg atgacgcctc acgtctttcg ctgatcgcga cacggggatc
     1321 cggctgcgca ctgaagtcgt agtcgagcct gggcgcgact tccaccactt cccagccgta
     1381 ggcgcagtgg aaccggctgt cggcggcgag cagtcgctgc agcagcgtgg tgccgctgcg
     1441 catcatgccg acgacgacga tcggcgcgat gatgctctcg tcggtgatct cggggtgcct
     1501 acgtatccat tcctcggcac gcaaccgcat ccgcaggctg tggaccagac tcgagcgcag
     1561 gatgtgcacg ccgatgtcgt tcaggtccgc ggcggagtag ctgtccgcga gcattgccaa
     1621 cggctcttcg aacggcaccg ggccccagtc atcgaggctc tccttgcgtt gcgcagcgct
     1681 catcagcccg ccggcgtcga aggcgtgtgt cacggtggtg agaacttcag gtgttggctg
     1741 gtgtcgccaa cctgatcgac gtacatgatg cgggtgtcga accaccacgt gccgtcgacg
     1801 cggtggaagg tgtcgcggta tcggccggtc acgatcactt gcaacggcag ttcaggcgtc
     1861 gcctgggtga cgcagtagta cgcgctgccg tctgcggttc cggcgtcctc gtcgacgtgc
     1921 agcgccacat tggtggtgag gtgcttggtc ttcggcgtgc cgtcttcgta gaggcgggtc
     1981 gccatctcat acatctgacg caccctggcg acgccggcga aaaccgtctc cgggggaccg
     2041 tcctccatgc cacagatgcg gccgtgagcg aacaacgccg ccaccccgtc caggtctccg
     2101 gcgtcgagac gttccgcgta ggtgtacacc aggttttcta tctcgcgggc gtcgtctctc
     2161 atcggcggta gacaaccagc cggaatgtgt catgtcaacg tatgcattca cgagtccgga
     2221 taggcgcagc actccggctc attgcgggat gcaattgttg ccgcccggat cactcgtcgc
     2281 atcccgcgat ggaccggccc aggtcgatgg tggctacgat ttccgaccgg gcttcgtcga
     2341 taaggtcatc cgcaccaatc gttccctgtc ctcattgctt ggccgatgct gtgtcgccgt
     2401 tgcacggcgc gccccataag ccgcgacggg cagttcgcgc ttgctgttcg gcccccgcga
     2461 tagcgtcata acggctgacc gggactccgc cgtagatata cgatctcgcg gcaccggcgc
     2521 gagcagcttc gaccgaatag tcccagccgt cctgccgcac gagatacccc agtgtgcggc
     2581 cgtagcggtc agtcttgtcc tgcgtagggt cggccacgag agccacccgc tggccgagca
     2641 tggtggattc cgcgaactcc gttgcttcgg gtccccagca gccgacggta tagcccggtt
     2701 tcttagtctc cggtgtgtca atccccaaga gccgcacgcg aagccggccc cgcacatcgt
     2761 cgcgaatgtc gatggtgtct ccgtcgacga ttttcagcac cgtcgctgtg acggtgacgg
     2821 gatcggcagt ggcggtc
//
LOCUS       DASYLX010000724         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2125168,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000724 DASYLX010000000
VERSION     DASYLX010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125168"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGP27_17000"
     CDS             complement(<1..310)
                     /locus_tag="VGP27_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7853030.1"
                     /translation="MTSAVGQPEIPVVDPSKLVPHKWKVLLGSGVIAIILGALMLVWP
                     GKTVLVATVLFGLYLLVVGGAQLFIAMASDLSGGGRALLFISGAVSVILAVLTFRYFG
                     E"
     gene            complement(443..1438)
                     /locus_tag="VGP27_17005"
     CDS             complement(443..1438)
                     /locus_tag="VGP27_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7853031.1"
                     /translation="MRGQEPGGPYFDDLEVGQAFDWAPSMTLTAGAAAVHQSIIGDRL
                     RLALDADLAIAVTGATTALAHPQLVCDVAIGQTTLATQRVKANLFYRGLSFHRFPVIG
                     DTLFTRTEVVALKQNAAKPGRPPTGLAALRMTTIDQIGRLVLDFHRCAMLPLRDGAAD
                     TGHADDVSSIGAEPALATDPTADWDADAFRARVPGTHFDSGIAGAVLHSTADVVTSAP
                     ELARLTLNIAATHHDSRAGGQRLVYGGHTIGLAFAQATRLLPNIATVLGWESCDHTGP
                     VHECDTLYSELHVDGAVPLPDHRGGVLKLRSLVYAAGEGADDRQVLDWRFTALQF"
     gene            complement(1441..>1978)
                     /locus_tag="VGP27_17010"
     CDS             complement(1441..>1978)
                     /locus_tag="VGP27_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827122.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7853032.1"
                     /translation="MMTPLHILDEYGNELPAGQAGEIYFEGGFDFEYLNDPEKTAKSR
                     DKHGWKTVGDIGYLDEDGYLYLTDRRHHMIISGGVNIYPQEAENMLVTHPKVMDAAVF
                     GIPDDEMGQSVKGVVQTVHSTDATGEFAAELLSWLRDRLSHYKCPRSISFEPQLPRTD
                     TGKLYKQELIAKYSHGKL"
BASE COUNT          345 a          634 c          656 g          343 t
ORIGIN      
        1 cctcgccgaa gtaccggaac gtcagcaccg caaggatcac cgacaccgcg ccgctgatga
       61 agagcagtgc ccgaccgcca cccgataaat cggatgccat cgcgatgaac agttgtgcgc
      121 cgccgacgac cagcagatac aggccgaaca ggacagtggc gacaaggact gttttacccg
      181 gccacaccag catcagcgcg ccgaggatga tcgcgatcac cccggacccg agcaacactt
      241 tccacttgtg cggcacgagt ttcgaggggt cgacgaccgg tatttccggc tgccctaccg
      301 cagaagtcac aagaacctcc cctaattgaa gtgattgtct ccgagaaagt cttctcggct
      361 gacgcggcca ccgcgtgcgc ccgtcagcac caaaatcgca cttccgactc gtcccggggg
      421 gatgactggc tcgggcgtcg ccctagaact gcagcgctgt gaaccgccag tccagtactt
      481 gcctgtcgtc ggcgccctca cctgccgcat aaaccagtga ccgcagcttg agcacaccgc
      541 cgcggtggtc gggcagtggc acagcgccgt cgacatgcaa ctcgctgtaa agggtgtcgc
      601 actcatgcac cggaccggtg tggtcgcacg actcccagcc gagcacggtc gcgatattgg
      661 gtagaaggcg agtggcctgg gcgaaggcca gcccgatggt gtggccgccg tagaccagac
      721 gctggccgcc tgcacgtgaa tcatggtgtg tcgctgcgat gttcaaggtg agccgggcca
      781 gttccggtgc gctggtgacg acatcggcgg tgctgtgcag cacggcgcct gcgatgccgg
      841 agtcgaagtg ggtgcccggc accctggcac ggaaggcgtc ggcgtcccag tcggcggtcg
      901 gatcggtcgc caatgcgggt tcggcgccga tggacgacac gtcgtcggcg tggccggtgt
      961 cggcggcgcc gtcgcgcagc ggcaacatgg cacagcggtg gaagtcgaga accagccggc
     1021 cgatctggtc gatcgtggtc atccgcagcg ccgcgagacc ggtgggcggc cggcccggct
     1081 tggccgcgtt ctgcttgagc gcgacgactt cggtgcgggt gaacagcgtg tcaccgatca
     1141 cggggaaccg gtgaaaagac aggccccggt agaacaggtt ggccttcacc cgttgggtgg
     1201 ccagcgtggt ctgcccgatg gcgacgtcgc ataccaattg cggatgagcg agcgcggtgg
     1261 tcgctccggt gaccgcgatc gcgagatcgg cgtcgagagc cagccgcaac cggtcgccga
     1321 tgatcgactg gtgcaccgcc gcagcgccgg ccgtcagcgt catcgacggt gcccagtcga
     1381 aggcctggcc gacttcgagg tcgtcgaagt agggtccgcc aggttcctga ccgcgcatcg
     1441 tcacagcttg ccatgcgagt acttcgctat cagctcctgc ttgtacagct tgccggtgtc
     1501 ggtgcgaggc agttgcggct cgaacgagat cgaccgcgga cacttgtagt gcgacaggcg
     1561 gtcccgcagc cagctgagga gctcagcggc gaactcgccg gtggcgtcgg tggagtgcac
     1621 cgtctgcacc acgcccttga cgctctgccc catctcgtcg tctgggatgc cgaagaccgc
     1681 ggcgtccatc acctttggat gggtgaccaa catgttctcg gcctcctgcg ggtagatgtt
     1741 caccccgccc gagatgatca tgtgatggcg cctgtcggtg aggtagaggt aaccgtcctc
     1801 gtcgagatac ccgatgtcgc cgacggtctt ccagccgtgc ttgtcgcgtg acttcgccgt
     1861 cttctccggg tcgttgaggt actcgaagtc gaagccgccc tcgaagtaaa tctcgcccgc
     1921 ctggcccgcg ggcaactcgt tgccgtactc gtcgaggatg tgcaacgggg tcatcatc
//
LOCUS       DASYLX010000725         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1795480,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000725 DASYLX010000000
VERSION     DASYLX010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795480"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1291
                     /locus_tag="VGP27_17015"
     CDS             68..1291
                     /locus_tag="VGP27_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853033.1"
                     /translation="MPARQLPYPVFDADNHFYEPKEALTQFLPDHRKGVIDYIDVRGR
                     TKIMVRNVVSDYIPNPTFEVVARPGAQEEYFRHGSGGKSYREVMGKPMKAIPAFRDPA
                     ARLEVLDALGLDYTLMFPTLASLVEERLKDDPDLILDIVHALNEWMYETWQFNYENRI
                     FSTPVINLGVVDRALEELDWCLERGAKTVLVRPAPVPGYRGTRSLGLEEFDPFWAACV
                     NAGIPVSMHASDSGYSEYLNDWEPGDEFKPFAPTSFRMVAMGKRPIEDTMAALVCHGA
                     LTRNPDLRILSIENGADWVPYLLKQFKSVYSKMPQGFLEDPIEAFRRCVYVAPFWEDD
                     FAQMADLCGVDRVIFGSDWPHPEGLAEPTKLVDDLHGLDEEGQRKVMGGNMIDLFKIP
                     NEVVHKPDVPALVLT"
     gene            1299..2432
                     /locus_tag="VGP27_17020"
     CDS             1299..2432
                     /locus_tag="VGP27_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853034.1"
                     /translation="MTEAANPEVLLFASTTQSFLEKEASLSRLRELHAAGIAFDAAWW
                     RRAAELGWTSLLVPESLGGGSVSGNGVKDLAVVAELAGRTVAPGPLHPVSTVLAGLVE
                     APENHEAAIESLVSGELVASWAVYEPGRPWAPLNPGMTATPTESGFRLDGAKDRVEAA
                     AESAVLLVVADCDGTVRQFLVPTDAPGVRVEPQRSVDLVKSYARVHFDGVEVDATAAV
                     GAAEQTAEMISRQAQIAQILQCAEVVGILDAVLTFTIQWGFDRHSFGRPLGSYQVLKH
                     RYADLKIWFEACKAATNAAVAEVAGRSPDAEMAVSVAKSYVGEYAPAMLQDCVQLHGG
                     IGVTWEHDLHLYLRRVMLDRSMFGTPEDHNLRVYALTADARSA"
     gene            2494..3747
                     /locus_tag="VGP27_17025"
     CDS             2494..3747
                     /locus_tag="VGP27_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853035.1"
                     /translation="MTETTSAPATTESVREFADRARVWLAENMPLIDPADPPDADRGE
                     EAPWLRARELQRKLYDGGFAGICFPREYGGLGLPIEYQRAFDEASRTYELPMILNTPT
                     FTICAATILDTGSEEQKKEHISGALRGDEVLVQLLSEPSGGSDLAGVITRADRHGDRW
                     VINGAKTWSTSAFAADYGLMLARTNWDVPKHEGLTMFLVPINSPGITLRRIKQVNGSV
                     EFCEEFFDNLELGDDAVVGEVNEGWHVASRQLYHERRAVGGGSEFASGIGAEGKTDVP
                     IDWVGLLEATGQADNERARERAGRAIVHKAVQERLIDHVYHGVLDGSLPPAAGSIIRI
                     THAETHQVGFDTAVALAGSAGVVEGDDDLLRFGERYLSRQTAALGGGTTEIARNIIGE
                     RVLGFPREAAADRGVPFKEVKRNKR"
     gene            complement(3782..>4388)
                     /locus_tag="VGP27_17030"
     CDS             complement(3782..>4388)
                     /locus_tag="VGP27_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV7853036.1"
                     /translation="MPGIIEALADSGTPFSILTKGTLLRRDLPLIADAAKRVDVSVAV
                     SLAVGDPALHRDVEPGTPTPQARLGLITAIRDAGLDCHVMVAPVLPKLTDTVEHLDNL
                     LNQIAAAGATGVTVFGLHLRGSTRGWFMSWLAHSHPELVADYRHLYRRGAYLPAEYRD
                     MLRDRATALINKHGLAPGRRSFRTKEASAALTADVAQPTLF"
BASE COUNT          777 a         1429 c         1458 g          724 t
ORIGIN      
        1 ttttcgctgg tcgtaaagtg gtaatgtcat taccactcca gcccaacgag ccttccagga
       61 ggcgaatatg ccggcccgcc agctcccgta tccggtgttc gacgccgaca accacttcta
      121 cgagcccaaa gaggcgctca cccagttcct gccggatcat cgcaagggcg tcatcgacta
      181 catcgacgtg cgtggccgca ccaagatcat ggtccgcaac gtggtcagcg attacatccc
      241 caacccgacg ttcgaggtcg tcgcccggcc aggcgctcaa gaggaatatt tccggcacgg
      301 cagcggtggc aagagctatc gcgaggtgat gggcaagccg atgaaggcga tccccgcatt
      361 ccgcgatccc gctgcgcgac tggaggtcct ggacgcgctc ggcctcgact acacattgat
      421 gttcccgacg ctggccagcc ttgtcgagga gcgtctcaaa gacgatcccg acctgatcct
      481 cgacatcgtt cacgcgctga acgaatggat gtacgagacg tggcagttca actacgagaa
      541 ccggatcttc tccaccccgg tcatcaacct cggcgtcgtc gaccgcgccc tcgaggaact
      601 ggactggtgc ctggagcgcg gcgccaagac cgtgctcgtc cggcctgcgc cggtacccgg
      661 ctaccggggc acccgctcgc tgggcctgga ggagttcgac ccgttctggg ccgcctgcgt
      721 caatgcgggg atccccgtgt cgatgcacgc ctccgacagc ggatattcgg agtatctgaa
      781 cgactgggag cccggcgatg agttcaagcc gttcgcgccg acctcgttcc ggatggtcgc
      841 gatgggcaag cgtcccatcg aggacaccat ggcggcactg gtctgccatg gtgcgctgac
      901 ccgcaacccg gatcttcgca tcctgtcgat cgagaacggc gcggattggg tgccctacct
      961 gctgaagcag ttcaagagtg tctactcgaa gatgccgcag ggttttctcg aggatccgat
     1021 cgaggcgttc cggcgctgcg tttacgtcgc gccgttctgg gaggacgact tcgcccagat
     1081 ggccgacctg tgcggtgtcg accgcgtgat cttcggttcc gactggcccc accccgaggg
     1141 cctggcggag ccgacgaagc tcgtcgacga cctgcacggc ctcgacgagg agggtcagcg
     1201 aaaggtgatg ggtggcaaca tgatcgacct cttcaagatt ccgaacgagg tcgttcacaa
     1261 gccagacgtg ccggccctcg ttctgacctg accgagccgt gaccgaagcc gcgaatcccg
     1321 aggtcctgct attcgcctcg accacccagt cgttcctgga gaaggaagcg tcgctgagcc
     1381 ggctgcgtga actgcacgca gccggtatag ccttcgacgc cgcctggtgg cggcgggcgg
     1441 ccgagctcgg ctggacgagc cttctcgttc ccgaatcgct tggcggcggt tccgtgtcgg
     1501 gcaatggcgt gaaggatctg gcggtggtgg ccgaactcgc aggcagaacc gtcgcgccag
     1561 gaccgctgca tccggtaagc accgtgctgg ccggactcgt cgaggcgccc gaaaaccacg
     1621 aggccgcaat cgaatcgctg gtgagcggcg agcttgtcgc atcgtgggcg gtgtacgaac
     1681 cgggcaggcc atgggcgccg ttgaatcccg gtatgacagc gacgcccaca gagtccggat
     1741 tccggctcga cggcgcgaag gaccgcgtcg aggcggccgc cgaaagcgcg gtgcttctgg
     1801 tggtggcgga ctgcgacggc acggtcaggc agtttctcgt gccgacggat gcgccaggcg
     1861 ttcgtgtcga accgcagcgg tcggtcgacc tcgtgaagag ctatgcccgt gtgcatttcg
     1921 atggcgtcga ggtcgatgcc acggcggccg tcggcgcggc ggagcagacg gcggagatga
     1981 tctcccggca ggcccagatc gcgcagatac ttcagtgcgc cgaggtcgtc ggcatcctcg
     2041 acgccgtgct gacgttcacc atccagtggg gcttcgaccg gcactcgttc ggccgcccgc
     2101 tcggctcgta tcaggtgctc aagcaccgct acgccgacct gaagatctgg ttcgaggcgt
     2161 gcaaggccgc caccaatgca gctgtggcgg aggtggccgg gcgctccccc gacgcggaaa
     2221 tggccgtcag cgtcgccaaa tcctatgtgg gcgaatatgc accggccatg ctgcaggact
     2281 gcgtccagtt gcacggcggc atcggcgtca cctgggagca cgacctgcat ctgtacttgc
     2341 gccgggtcat gctcgaccgc tcgatgttcg gcacacccga ggaccacaat ctgcgcgtct
     2401 acgcgctcac cgcggacgcg aggagcgcct gatgaccaac gagcgcttgc gcgtgggggc
     2461 acctcccacc cgcagggtag ggggagagcc gacatgaccg agacgacgtc ggcgcccgcc
     2521 acaaccgaat ccgtccgtga attcgccgat cgggcgcggg tctggctcgc ggagaacatg
     2581 ccgctcatcg acccggccga tccgccagac gcggatcgcg gcgaggaagc gccgtggctg
     2641 cgtgcccgcg aactccaacg caagctctat gacggcgggt tcgcaggcat ctgtttcccg
     2701 cgcgaatacg gcgggctcgg actgcccatc gaatatcagc gggcgttcga cgaagcctcg
     2761 cgaacctacg agctgccgat gatcctcaac acaccgacgt tcaccatctg tgccgccacg
     2821 attctcgaca ccggcagcga agaacagaag aaggagcaca tctcgggcgc gctgcgcggt
     2881 gacgaggtgc tcgtgcagct tctgtcggag cccagtggcg gatccgatct tgcaggtgtg
     2941 atcacccgcg ccgaccgaca cggcgacaga tgggtgatca acggcgcaaa gacctggagc
     3001 acaagtgctt tcgccgccga ctacgggctg atgctggccc gaaccaattg ggacgtgccc
     3061 aagcacgagg gcctgacgat gttcctggtt ccgatcaaca gtcccggcat caccttgcgc
     3121 cggatcaaac aggtgaacgg ctcggtcgag ttctgtgagg agttcttcga caacctcgaa
     3181 ctcggtgacg acgccgtcgt cggcgaagtc aacgagggct ggcacgtggc gtcccggcag
     3241 ctgtatcacg agcggcgcgc tgtcggcggc ggttcggagt tcgccagcgg gatcggcgcc
     3301 gagggcaaga ccgacgtgcc gatcgactgg gtcgggcttc tcgaggcgac gggccaggcc
     3361 gacaatgagc gggcccgcga gcgggccggt cgggcgatcg tgcacaaggc ggtccaggaa
     3421 cgcctgatcg accacgtcta ccacggcgtg ctcgacggct cgttgccgcc ggcggcggga
     3481 tccatcatcc ggataaccca cgctgagacc catcaggtgg gattcgacac cgcggtcgcg
     3541 cttgcgggca gcgcgggcgt cgtcgaaggc gacgacgatc tgctccggtt cggtgagcgc
     3601 tacctgtcga ggcagacggc cgcgctcggt ggcggcacga ccgagatcgc ccgcaacatc
     3661 atcggtgaac gggtgctcgg gttcccgcgt gaggcggccg ccgaccgcgg cgtgccgttc
     3721 aaagaggtca aacgcaacaa gcgctgaact ccatcgtcga gtctgatcgt cgagtctgaa
     3781 atcagaacag agtgggctga gccacgtcgg ccgtcaaggc cgcggacgcc tccttcgttc
     3841 gaaacgatcg ccggccaggc gcaagcccgt gcttgttaat gagcgcagtc gccctgtcgc
     3901 gcaacatgtc tcggtactcg gctggcaggt aggctccgcg ccggtacagg tgccggtagt
     3961 cggcgaccag ctcggggtgc gagtgtgcca gccacgacat gaaccagccc cttgtcgatc
     4021 cccgcagatg cagcccgaac accgtcacgc cggtcgcgcc cgcagcggcg atctggttga
     4081 gcagattgtc gagatgctcg accgtgtcgg tcagcttcgg cagcaccggc gcgaccatca
     4141 catggcagtc aaggccggca tcgcggatcg cggtgatcag gcccagccgc gcctgcggcg
     4201 taggcgttcc cggctcgacg tccctgtgca gcgcggggtc tcccacggcg agcgacaccg
     4261 cgacactgac gtcgacccgc ttggccgcgt cggcgatcag cggaaggtca cgcctcagca
     4321 gcgtgccctt ggtgagaatg gaaaacggtg tgccggaatc cgccagcgcc tcgatgattc
     4381 ccggcatc
//
LOCUS       DASYLX010000726         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3878628,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000726 DASYLX010000000
VERSION     DASYLX010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878628"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VGP27_17035"
     CDS             <1..172
                     /locus_tag="VGP27_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853037.1"
                     /translation="PVRWWHGDADNIVPLRDAQAAIDLLPDAELVLRREESHLGGFAT
                     ADEVLVFLRSHL"
     gene            complement(504..701)
                     /locus_tag="VGP27_17040"
     CDS             complement(504..701)
                     /locus_tag="VGP27_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853038.1"
                     /translation="MAVTVTLRDRATDKYMRFGDSYVKHNDGGLDVVRGGAKQPYSHA
                     PGDWADVEGNEKSWKKGLFWG"
     gene            complement(780..938)
                     /locus_tag="VGP27_17045"
     CDS             complement(780..938)
                     /locus_tag="VGP27_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6307 family protein"
                     /protein_id="HEV7853039.1"
                     /translation="MASPTMPRTPYDLRVQLVEDIIKRHSKIGNEAAGELAVQILLAL
                     NSIPEKIR"
     gene            complement(1044..>1610)
                     /locus_tag="VGP27_17050"
     CDS             complement(1044..>1610)
                     /locus_tag="VGP27_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV7853040.1"
                     /translation="GRDPTVLYRHVPGKAALLDGVAEIVLGQLRVDTADPDWASQLRT
                     VAHEFRRLAVTHPNVVPLLVTRPLTTPLGQRPPGMLRPLEDVVALLTSSGFSGADALH
                     IYRVLFGYLHGHILTELQEVIERPEETDDVLRLGLHRLAVTEFPQVRALASVLASYDG
                     EAELDRGLDLLFFGLTATLTTPDGSSRS"
BASE COUNT          301 a          532 c          479 g          298 t
ORIGIN      
        1 gccggtgcgg tggtggcacg gcgacgccga caacatcgtg ccgctgcgcg acgcgcaagc
       61 cgcgatcgac ctactgcccg acgccgaact cgtcttgcgc cgcgaggaaa gccacctcgg
      121 cgggttcgcc accgccgacg aagtcctggt cttcctccgc tcccatctct aatcggtgtg
      181 tgtccaccga acacctacac cacctgtcga aatgtcgtgg cacactccaa ttccgagccg
      241 cgcatcaaca tgcccggcga gaattggcag acgggctgga taaagttcgc tcacccgttg
      301 ccgcgaccgg cacgcgtcgc ggattagtcg cattgaagac ttcacgccct gcaggcacgt
      361 cacagcattt tgggctcagt ggcaattccg tgacacgcac ccgacacaaa taacttgtcg
      421 ctgcaatccg gcaccgcgtc acagcgaccc gccgagcccg tcgatcatcc ccggcgaagg
      481 gctccacgac cacgggtgat cggtcagccc cagaaaaggc ccttcttcca actcttttcg
      541 ttgccttcaa cgtcggccca gtcccctggc gcgtggctgt acggctgttt cgcaccgccg
      601 cggacgacat cgagcccgcc gtcgttgtgc ttgacgtagg aatcgccgaa ccgcatgtac
      661 ttgtcggttg cgcggtcgcg gagtgtcacg gtaactgcca ttgtcgaagc ctccagatgc
      721 gccggccaat ggcggcaagg gtcagatcaa aaatgtgagt gggatgccgc gggccgctgt
      781 cagcgaatct tctcggggat tgaattcagc gcgaggagga tctgcacggc caattcgcct
      841 gcggcctcgt taccaatctt cgagtgcctc ttgatgatgt cctcgacaag ctggacgcgt
      901 aggtcatagg gcgtgcgggg cattgttggc gaagccatga attttccttc atagtcgagc
      961 atcttcgctc gcaaccgagc ggcccgatag tcctgacggt acgccctttt cgtcacgtgc
     1021 gttgtgtagc ccgttgcccc ccatcacgat cgagacgacc cgtccggcgt cgtcagtgtc
     1081 gccgtgagtc caaagaacag cagatcgagg ccgcggtcga gctcagcctc accgtcgtag
     1141 gacgccagca ccgatgccaa ggcgcgcacc tgtgggaact cggtgaccgc cagccggtgc
     1201 aagcccagcc gcaacacatc atcggtctcc tccggtcgtt cgatcacctc ttgcagctcg
     1261 gtgaggatgt ggccgtgtag atagccgaag agcacccggt agatgtgcag ggcatctgcc
     1321 ccgctgaagc cggaggacgt gagcagggcg acaacatcct cgagaggccg cagcatcccc
     1381 ggcggccgct gtcctaatgg agtcgttagc ggccgagtca ccaacaacgg caccacgttt
     1441 gggtgtgtga cggccaaccg gcggaactcg tgggcgacgg tgcgcaactg actcgcccag
     1501 tctggatcgg ccgtatctac ccgtagttga ccgagcacga tctccgcgac accgtctagc
     1561 agcgccgcct tacccggtac gtgtcgatag agcactgtcg gatcccggcc
//
LOCUS       DASYLX010000727         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_478193,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000727 DASYLX010000000
VERSION     DASYLX010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478193"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGP27_17055"
     CDS             complement(<1..1193)
                     /locus_tag="VGP27_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7853041.1"
                     /translation="MRRLRLARSASLRTRVALAAATAAAAVVAVFTILTSLVLANNDA
                     AQLDRRLDSIVDASMYPDQLSNPRQGVLTTGRMESTGQVVFQRGLQLPRLQPGTADVV
                     VNGVDYRVRTIPVDQQGGVLMSIGIRADSILLNRARIPFYVAVGLVTVLIAAGVGWLL
                     SGPAIRPLRRLTEHTRRLGKGTEEMPEVRGIREAEELSEAMTGMLSRLAAAQQATQNS
                     LQAAQDFAANAAHELRTPLTAMRADLDTLRLHDLPEEERAEVVMDLSRAQRRVEAIIT
                     ALGQLASGELAQAEDREVIDVTDLLDRVARENMRPGGSMQVVVEADEHLGTIWGWPSG
                     LRLAVDNLVRNAVAHGSASTVLLTGHRIDHSITITVDDNGGGLPAEEHHIVLGRFARG
                     STAAPG"
     gene            complement(1333..>1648)
                     /locus_tag="VGP27_17060"
     CDS             complement(1333..>1648)
                     /locus_tag="VGP27_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV7853042.1"
                     /translation="NQSSDTMSVGPLTIDTARRLVFVDGERVDLTKREFDLLAVLAEN
                     AGVVLSRQRLLELVWGYDFDVDTNVADVFISYLRRKLEREGLPRVIHTVRGIGYVLRD
                     ET"
BASE COUNT          263 a          569 c          521 g          295 t
ORIGIN      
        1 ccgggcgcgg cggtggatcc gcgtgcgaag cggccgagca cgatgtgatg ttcctcggcc
       61 ggcagaccgc caccgttgtc gtcgaccgtg atcgttatcg agtgatcaat ccggtgacct
      121 gtcagcagca ccgtggacgc cgatccgtgt gccacggcgt tgcgcaccag gttgtcgact
      181 gccaggcgca ggccgctcgg ccagccccag atggtgccga gatgctcgtc cgcctcgacg
      241 acgacctgca tcgacccacc cgggcgcatg ttctcccgcg ccacccggtc gagcaggtcg
      301 gtcacgtcga tgacttcgcg gtcctccgcc tgcgccagct cacccgacgc cagctgaccc
      361 agcgccgtga tgatcgcctc gacgcgacgc tgcgcccgcg acaggtccat gaccacctcg
      421 gcccgctcct cttcaggtag gtcgtgaagc cgcagcgtgt ccaggtcggc acgcatcgcg
      481 gtcagcggtg tgcgcaactc gtgtgcggcg ttcgccgcga aatcctgtgc ggcctgcagg
      541 gagttctggg tggcctgctg cgcggcggcg agccggctga gcattcccgt catcgcctcg
      601 gacagttctt cggcctcgcg gataccgcgt acctccggca tctcctcggt gcccttgccg
      661 agccgtctgg tgtgttccgt gagccgccgc agcgggcgga tcgcgggacc cgacaacagc
      721 catcccaccc ccgccgcgat caacaccgtg acgaggccga ccgcgacgta gaacggtatg
      781 cgggcccggt tcaacaggat gctgtcggcc ctgatgccga tcgacatcag cacgccgcct
      841 tgctggtcca caggaatggt gcgcactcga tagtcgacgc cgttgacgac gacgtcggcc
      901 gtgcccggct gcaggcgcgg cagttgaagc ccgcgctgaa aaaccacctg gccggtcgac
      961 tccatccggc ctgtcgtcag cactccctgc cggggattcg acaactggtc ggggtacatg
     1021 ctggcatcga cgatggagtc gagccgccga tccagttgtg cggcatcgtt attggcgaga
     1081 accagcgatg tcagaatggt gaacaccgcg acgacggcgg cggccgcggt agcggcggcc
     1141 agcgccaccc tggtgcgcag cgaagcggaa cgcgccaaac ggaggcggcg cactacgttt
     1201 cgtctcggtg ctggatgctc gacttcacta cgtttcgtct cggtgctgga tgctcgactt
     1261 cactacgttt cgtctcggtg ctgggtgctc gacttcacta cgtttcgtct cggtgctgga
     1321 tgctcgactt cactacgttt cgtctcgcag cacatagccg atccctcgaa ccgtgtggat
     1381 cacccggggc aaaccctcgc gttccagttt gcgccgcaag tagctgatga agacatcggc
     1441 cacgttggtg tccacgtcga agtcgtaccc ccacaccagt tcgagcagtc gctgccggct
     1501 gagcacgacg cccgcattct cggccagcac cgccagcagg tcgaactccc gcttggtcag
     1561 atcgacccgc tcaccgtcga cgaacaccag tcgccgagcg gtgtcgatgg tcagcggacc
     1621 gaccgacatg gtgtccgacg actgattg
//
LOCUS       DASYLX010000728         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1026629,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000728 DASYLX010000000
VERSION     DASYLX010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026629"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /gene="purL"
                     /locus_tag="VGP27_17065"
     CDS             complement(<1..1605)
                     /gene="purL"
                     /locus_tag="VGP27_17065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEV7853043.1"
                     /translation="MVDSVERAAATPDQPQPFRELGLKDDEYERIREILGRRPTDSEL
                     AMYSVMWSEHCSYKSSKVHLRYFGETTTEKMRSAMLAGIGENAGVVDIGDGWAVTFKV
                     ESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTRRVVDGVV
                     RGVGGYGNSLGLPNIGGETVFDASYGGNPLVNALCVGVLRKEDLHLAFASGTGNKIIL
                     FGARTGLDGIGGVSVLASETFGGDEDGAGRKKLPSVQVGDPFMEKVLIECCLELYAAG
                     LVIGIQDLGGAGLSCATSELASAGDGGMAVELDKVPLRAANMTPAEILSSESQERMCA
                     VVAPENVDAFMAVCRKWEVLATVIGEVADGDRLTVTWHGETVVDVPPRTVAHEGPVYE
                     RPVQRPDTQDALNADTSDTLPRPKTGEELRATALALIGSPHLCSRAFITEQYDRYVRG
                     NTVLAENADGGVLRIDESTGRGIAISTDASGRYTQLDPYAGAQLALAEAYRNVAVTGA
                     TPIAVTNCLNFGSPEDPGVMWQFSEAV"
     gene            complement(1677..2885)
                     /locus_tag="VGP27_17070"
     CDS             complement(1677..2885)
                     /locus_tag="VGP27_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7853044.1"
                     /translation="MLASVIAVALVLVAWSLLARRLERWRITAPMFLVLAGIAVGLST
                     QDVLVESLNTDAAQRAAEIVLAVLLFVDATDIRGGLFGSQPRAAVRVLFIAMPLSLAL
                     SVALGLWLLPGLGWAVLLVIACVVVPTDFAPAPSVLRDERVPERVRDVLNVEAGYNDG
                     IVSPVFIFALVLAGDRSHAATPMEALGSAVPQAVKAILVGVAIGAALAVLTNAAERRG
                     EMTDQSKRLILVAAPVLAYGSSLAVGGNGFVCAFVCGFAFNYLRRSQAIRAELELLDD
                     IGFLLSAGMWFVFGATAVLTLTFGVSWGMLLFCLLALTVVRLLPVLVAMLGSRFSWAE
                     RLLIGWLGPRGTTSIVFGLLAFNVLAGEDERAVLMAMVIAVLGSVMLHGVGAPAVARG
                     YRSSQTRMGG"
     gene            complement(2919..>3179)
                     /locus_tag="VGP27_17075"
     CDS             complement(2919..>3179)
                     /locus_tag="VGP27_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7853045.1"
                     /translation="QLNPVAPPFFVVVSLPEGPGLAFQKVDDPTPGKNRVHVDFSTSD
                     MEAEVKRLVGLGASETGRGSFGDFEWVVLADPDGNAFCVAGG"
BASE COUNT          554 a         1110 c         1042 g          473 t
ORIGIN      
        1 gacggcttcg gagaattgcc acatgacacc tggatcctcc ggcgaaccga agttcaggca
       61 gttggtgacc gcgatcgggg tggcgccggt caccgcgacg ttgcggtatg cctcggccag
      121 cgcgagttgc gcgcccgcgt acgggtccag ttgggtgtag cggcccgagg cgtcggtgga
      181 tatcgcgatg ccgcgtcccg tggactcgtc gatacgcagc accccaccgt cggcgttctc
      241 ggccagcacg gtgttgcccc gaacgtagcg gtcgtactgc tcggtgatga atgcccggct
      301 gcacaggtgc ggactgccga tcagcgcaag cgcagtcgcc cgcaactcct caccggtttt
      361 cggccgcggc agcgtgtcgg acgtgtcggc gttgagcgca tcctgggtgt ccggccgctg
      421 cacgggccgt tcgtagacgg ggccttcgtg cgcgaccgtg cggggcggca cgtcgacgac
      481 cgtctccccg tgccaggtga ccgtcagccg gtcgccgtcg gcaacctcgc cgatgactgt
      541 ggccagcacc tcccacttgc ggcacaccgc catgaacgcg tcgacgttct cgggggcgac
      601 gacggcgcac atccgttcct gcgactcgct ggacaggatc tccgctggcg tcatgttggc
      661 cgcccgcagc ggtaccttgt cgagttcgac ggccatacca ccgtcgcctg ccgaagcgag
      721 ttccgatgtc gcgcaggata atccggcgcc accgaggtcc tggatgccga tcaccaggcc
      781 ggctgcgtag agctcgaggc agcactcgat gaggaccttt tccatgaacg ggtcgccgac
      841 ctgcaccgag ggcagcttct tacggcctgc gccgtcttcg tcgccgccga atgtctcgga
      901 agccagtacc gaaacaccgc cgatcccgtc gaggccggtg cgggccccga acaggatgat
      961 cttgttgccg gtgcccgacg cgaacgccag gtgcagatcc tctttgcgca acaccccgac
     1021 acacagggcg ttcaccagcg gattgccgcc gtaggaggca tcgaagactg tttcgccgcc
     1081 gatgttgggc agtcccaacg agtttccgta tccgccgacc ccgcgaacca cgccgtcgac
     1141 gacgcggcgg gtgtcggggg catcagcggc gccgaaccgc agctggtcca tcaccgccac
     1201 cggtcgggcg cccatcgcca tgatgtcgcg gacgattccg ccgacaccgg tcgcggcgcc
     1261 ctggtacggc tcgacgtatg acgggtggtt gtgcgattcg accttgaagg tgaccgccca
     1321 gccgtcgccg atgtcgacga caccggcgtt ctcgccgata ccggccagca tcgccgagcg
     1381 catcttctcg gtggtggtct caccgaagta gcgcaggtgc accttcgagg acttgtagct
     1441 gcagtgctcg ctccacatca ccgaatacat cgcgagttcg gaatcggttg gtctgcggcc
     1501 gaggatttcc cggatgcgct cgtactcgtc gtctttgagg ccgagctctc ggaaaggttg
     1561 cggctggtcg ggggtggctg ccgctctctc gacggagtcg acgatcgggc ttgggccgac
     1621 ggtgtcgacc aacgtagttg cgccgacgga gtcggcggtc tggctgagct ccgaggtcac
     1681 ccccccattc tagtttgaga gctgcgatat ccgcgggcga ccgcgggcgc gccgaccccg
     1741 tgcagcatca cactgcccag caccgcgatg accatggcca tcagcaccgc gcgctcgtcc
     1801 tcgccggcga gcacgttgaa cgccagcagc ccgaacacga tggaggtggt acctcgcggg
     1861 ccgagccagc cgatcagcag ccgctcggcc cacgagaatc tcgagccgag catcgcgacc
     1921 agcaccggca acaaccgcac caccgtcaac gccagcaggc aaaacagcag catgccccac
     1981 gacacaccga acgtcagggt cagcaccgcg gtggccccga acacgaacca catgccggcc
     2041 gacagcagga agccgatgtc gtcgagtagc tccaactcgg cgcggatcgc ctgggagcgg
     2101 cgcaggtagt tgaacgcgaa cccgcagacg aaggcacaga cgaagccgtt tccaccgacg
     2161 gccaggctcg acccgtacgc cagcaccggg gcggccacca gaatcaggcg cttgctctga
     2221 tcggtcatct ccccgcgccg ctcggcggcg ttggtgagca cagccagcgc cgcgccgatc
     2281 gcgacaccga cgaggatcgc cttgacggcc tgcggcacgg ccgaacccag cgcctccatc
     2341 ggtgtcgccg cgtgggaacg gtcgccggcg aggaccaggg cgaagatgaa caccggcgag
     2401 acgatgccgt cgttgtaacc ggcttcgaca ttgagcacgt cgcggacccg ctcgggcacc
     2461 cgctcgtcgc gcagtaccga cggggcgggg gcgaagtccg ttggcaccac aacgcacgcg
     2521 atgaccagca ggaccgccca cccgaggccc ggcagcagcc acagtcccag ggccaccgac
     2581 agcgccaggc tcagtggcat cgcgatgaac agcacacgca ccgccgcgcg cggctgactc
     2641 ccgaagagcc cgccgcgaat atcggtggcg tcgacgaaaa gtaggacagc gaggacgatt
     2701 tccgcggcgc gctgagcggc gtctgtgttc agcgactcga ccagcacgtc ctgggtggac
     2761 aagccgaccg cgatgccggc cagcaccagg aacatcggcg cggtgatacg ccagcgctcg
     2821 agccggcggg ccagcagcga ccacgcgacc aggacgagcg ctaccgcaat gaccgacgcc
     2881 agcacgggcg cgattgtgcc cggtgcgccg gtcgtccgct agccgcccgc cacgcagaac
     2941 gcgttgccgt cgggatcggc gagcaccacc cattcgaaat cgccgaagct gcctctgccg
     3001 gtttcgctgg ccccgaggcc gaccagccgc ttcacctcag cctccatgtc ggaggtgctg
     3061 aagtcgacgt gcacgcggtt cttacccggc gtgggatcat cgaccttctg gaacgccaga
     3121 cccgggccct ctggcagcga cacgacgacg aaaaacggtg gagccacagg gtttagctg
//
LOCUS       DASYLX010000729         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3550069,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000729 DASYLX010000000
VERSION     DASYLX010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550069"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGP27_17080"
     CDS             complement(<1..260)
                     /locus_tag="VGP27_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853046.1"
                     /translation="MPRPVALITGPTSGLGAGFARRYAVDGYDLVLVARDVERLERLA
                     AELHDEAGAAVEVLPADLADAADRGKVADRLSEGVRVLVNNAG"
     gene            complement(274..1074)
                     /gene="ttfA"
                     /locus_tag="VGP27_17085"
     CDS             complement(274..1074)
                     /gene="ttfA"
                     /locus_tag="VGP27_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose monomycolate transport factor TtfA"
                     /protein_id="HEV7853047.1"
                     /translation="MVPLWFTLSALCFVGAAVLLYVDIDRRRGMGRRRKSWAKSHGFD
                     YEHESDDLIKRWKRGVMSTVGDVSAKNVVLGQIRGEAVFIFDLEDVATVIALHRKVGT
                     NVVVDLRLKGIKEPRESDIWLLGAIGPRMVYSTNLDAARRACDRRMVTFAHTAPDCAE
                     IMWNEQNWTLVSMPVTSTRAQWDEGLRTVRQFNDLLRVLPPTPQTGAAGAAAAAQPAR
                     RAAAPGRPVKPPATDARYAQPRTDANRSDPAIRRQPPRNGRQSPHYQR"
     gene            complement(1167..1640)
                     /locus_tag="VGP27_17090"
     CDS             complement(1167..1640)
                     /locus_tag="VGP27_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853048.1"
                     /translation="MTTHETRPEDRPVQLGAPEDQAVPLGTTTSHPDDVGTVGPTGDF
                     GRAQEAGASPPQQAAESTATAGSADRTLFADGDLTDLRLRWTEVQSAFVDDPRDCVQK
                     ADGLVADVVDRLTAGFAQARSGLEEQWSRGEEVSTEDLRLALQRYRDFFERLLAV"
     gene            complement(1627..2220)
                     /locus_tag="VGP27_17095"
     CDS             complement(1627..2220)
                     /locus_tag="VGP27_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853049.1"
                     /translation="MSEWVWVLIAVLVLVVVAVAGWSVARRRRSERLKEHFGPEYDRT
                     VGDLGEQRAAEAELLAREKKRKKLDIVDLTPEVRQEHAVVWARVQTDFVDDPQDAVGR
                     AERLVTRVMRERGYPIDDFDHRAADISVDHPDIVENYRGAHAIYQSQHDGDISTEDAR
                     QAFVHYRALFDRLLGSDDDVDTHVRTNEQERQAHDHA"
     gene            complement(2403..>3689)
                     /locus_tag="VGP27_17100"
     CDS             complement(2403..>3689)
                     /locus_tag="VGP27_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7853050.1"
                     /translation="RRLEIEEMALAKETDAASVDRLEKLRAELADKREQLAELTTRWQ
                     NEKNAIDVVRELREQLEQLRGEADRAERDGNLEKAAELRYGRIPEVEKKLDAALPQAE
                     AREDVMLKEEVGPDDIADVVSAWTGIPAGRMLEGETAKLLRMEDELGRRVVGQKRAVQ
                     AVSDAVRRSRAGVADPNRPTGSFMFLGPTGVGKTELAKALAEFLFDDERAMVRIDMSE
                     YGEKHSVARLVGAPPGYIGYDQGGQLTEAVRRRPYTVVLFDEIEKAHPDVFDVLLAVL
                     DEGRLTDGQGRTVDFRNTILILTSNLGAGGSEEQVMAAVRSAFKPEFINRLDDVIVFD
                     GLNPEELVQIVDIQLQQLAKRLAARRLTIEVSLPAKKWLAERGFDPLYGARPLRRLIQ
                     QAIGDQLAKMLLSGDVHDGDIVPVNVSPDGDGLVLG"
BASE COUNT          555 a         1299 c         1193 g          642 t
ORIGIN      
        1 ccggcgttgt tcaccagtac ccgaacccct tcggacagcc ggtcggcgac tttgccgcgg
       61 tcggccgcgt cggccaggtc ggcggggagc acctcgacgg cggcgcccgc ctcgtcatgc
      121 agttcggcgg cgagccgttc cagccgttcg acgtcgcggg cgaccaatac caggtcgtat
      181 ccgtcgacgg cgtagcgccg ggcgaaaccc gccccaagcc cggatgtcgg tccggtgatc
      241 aaagcgacgg ggcgcggcat gcagacaggt tactcagcgc tgatagtgcg gcgactggcg
      301 gccgtttcgc ggtggttggc gacggatcgc cgggtcggag cggttggcat ccgtacgcgg
      361 ctgggcgtaa cgcgcgtcgg tcgcgggcgg cttgaccggc cggccgggcg ccgcggcccg
      421 gcgtgcgggc tgcgccgccg ctgcggcacc ggcggcgccg gtctgcggag tcggcggcaa
      481 cacccgcaga aggtcgttga actgacgcac ggtgcgcagc ccttcgtccc actgcgcccg
      541 cgtgctggtg acgggcatgc tgaccagcgt ccagttctgc tcgttccaca tgatctcggc
      601 acagtcgggc gcggtgtggg cgaacgtcac catccgtcgg tcgcaggcgc gccgcgccgc
      661 atcgaggttg gtggagtaca ccatccgcgg gccgatcgcc ccgagcagcc aaatgtcgct
      721 ttcgcgcggc tccttgatgc ccttgagccg caggtcgacc acgacgttcg taccgacctt
      781 gcggtgcagg gcgatcaccg tcgcgacgtc ttcgaggtcg aagatgaaca cggcctcgcc
      841 gcggatctgg cccagcacca cgttcttggc gctgacgtcg ccgacggtcg acatgacgcc
      901 gcgcttccag cgcttgatca ggtcatcgga ttcgtgttcg tagtcgaagc cgtgcgattt
      961 ggcccatgac ttgcgccgac gccccatgcc gcgtcgacgg tcgatgtcga cgtacaacaa
     1021 cactgccgca cccacgaaac agagtgcgga cagcgtgaac cagaggggaa ccattacccc
     1081 tagcctactt gcaggtaggt cagatgccag aactcatcca agtcacaacc ccgttactta
     1141 tggccgcagc gccgtcggcg tcgcactcag accgcgagga ggcgctcgaa gaagtcgcgg
     1201 taccgctgca gggccagccg gagatcctcc gtggagacct cctcgccgcg gctccactgc
     1261 tcctccagcc cggagcgggc ctgcgcgaac ccggccgtca gccggtcgac cacgtcggcc
     1321 accagcccgt cggccttctg cacacagtcg cgcggatcgt cgacgaacgc cgattgcacc
     1381 tcggtccagc gcaagcggag atccgtcagg tcaccgtcgg cgaacagcgt ccggtcggct
     1441 gaacccgcgg tcgccgtcga ctcggcagcc tgctgcggcg gcgaagcgcc ggcctcctga
     1501 gcgcgcccga aatcacccgt ggggccgacg gttccgacgt cgtcgggatg gctcgtcgtc
     1561 gtccccagag gcaccgcctg gtcctcgggt gcaccgagtt gcaccggccg atcctcgggt
     1621 cgagtctcat gcgtggtcat gggcctgcct ctcttgctcg ttggttcgga cgtgggtgtc
     1681 gacgtcgtcg tcggacccga gtagccggtc gaacagtgcg cgatagtgca cgaacgcctg
     1741 gcgggcgtcc tcggtgctga tgtcgccgtc gtgctgggat tgatagatgg cgtgcgcgcc
     1801 gcggtagttc tcgacgatgt cgggatggtc gacggagata tcggccgcgc gatggtcgaa
     1861 gtcgtcgatc gggtagccgc gctctcgcat cacccgggtc accaggcgct cggcccgccc
     1921 gaccgcatcc tgcgggtcgt cgacgaaatc ggtctgcacc cgcgcccaca cgaccgcgtg
     1981 ttcctggcgc acctcgggtg tcaggtcgac gatgtcgagc ttcttgcgct tcttctcgcg
     2041 tgcgaggagt tcggcttctg cggcccgttg ttctcccagg tcgccaaccg tgcggtcata
     2101 ttcgggaccg aagtgctcct tcagccgctc cgacctcctt cgccgggcga ccgaccaccc
     2161 cgcgacggcc acaacgacca gtaccagcac tgcgatgaga acccataccc actcggacat
     2221 ttcgttgacc tcctcgtcaa agagaggagt aaccgcgcaa tctccgacgg aaacgtcaat
     2281 ttgtgatccg cgtcgcagtg cgtctgttga cggccgacgt tgactctgcg cacatggcgc
     2341 gaaagtgcgc tcggaattcg cgcactcagc gcagagtcaa cgccgatcta tctagggcga
     2401 aatcagccca gcacgagccc gtcgccgtcc gggctgacgt tgaccggcac gatgtcgccg
     2461 tcgtgcacgt cgccggacag cagcatcttc gccaactggt cgccgatggc ctgctggatc
     2521 agcctgcgca gcggacgcgc gccgtacagc ggatcgaaac cgcgctcggc cagccacttc
     2581 ttggccggca gcgacacctc tatcgtgagc cggcgtgcag ccagccgctt cgccagctgc
     2641 tgcagctgga tatcaacaat ctggacgagc tcctccgggt tgagcccgtc gaagacgatc
     2701 acgtcgtcca gccggttgat gaactccggc ttgaacgccg agcgcaccgc ggccatcacc
     2761 tgctcctcgg agccaccggc gccgaggttc gacgtcaaga tcaggatcgt gttgcggaag
     2821 tcgaccgtgc ggccctgccc gtcggtcaac cggccctcgt ccagcacagc cagcagcacg
     2881 tcgaacacgt ccgggtgagc cttctcgatc tcgtcgaaca ggaccaccgt gtacggacgc
     2941 cgccgcaccg cctcggtcag ctgaccgccc tggtcgtagc cgatgtagcc gggaggcgca
     3001 ccgacgaggc gggccaccga gtgcttctcg ccgtactcgc tcatgtcgat gcggaccatc
     3061 gcccgttcgt cgtcgaagag gaactccgcc aacgcttttg cgagctcggt cttgccgacg
     3121 ccggtcgggc cgaggaacat gaaggagcct gtcggccggt tcgggtcagc gacgcctgcc
     3181 cggctgcggc gcaccgcatc ggacaccgcc tgtacggccc tcttctgacc gacgacgcgc
     3241 ctgccgagct cgtcttccat ccgcagcagc ttggccgtct cgccctcgag catgcgcccg
     3301 gccgggatgc cggtccacgc cgacaccacg tcggcgatgt cgtcgggccc gacctcttct
     3361 ttgagcatga cgtcctcgcg cgcctccgcc tgagggagcg ccgcgtcgag cttcttctcg
     3421 acctcgggga tacggccgta gcgcagctcg gccgccttct ccaggtttcc gtcgcgttcg
     3481 gcgcgatcgg cctcgccgcg cagctgctcg agctgttccc tgagctcgcg gaccacgtcg
     3541 atggcattct tctcgttctg ccaacgggtg gtcagctcgg ccagttgctc cctcttgtcg
     3601 gccagttcgg cgcgcagctt ctccagcctg tcgacggatg ctgcatccgt ctctttggcg
     3661 agcgccatct cctcgatctc gaggcggcg
//
LOCUS       DASYLX010000730         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3659943,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000730 DASYLX010000000
VERSION     DASYLX010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3659943"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1788
                     /locus_tag="VGP27_17105"
     CDS             25..1788
                     /locus_tag="VGP27_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007317772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853051.1"
                     /translation="MALSNTTLGVLMATMNSSIVLISLPAIFRGIHIDPLQPGNISYL
                     LWMIMGYMLVTAVLVVTLGRLGDMYGRAKIYNAGFAVFTLASILLAVEPLGGGGGAMW
                     LIIVRVLQGIGGAMLMANATAILTDAFSADRRGLAVGINAVAAIGGSFIGLLVGGVLS
                     EVDWRLVFWVSVPFGVIGTIWAYLSLHDTNVRRSHKIDWPGNITFAIGLILLLVGIVY
                     VIQPYGGHAEGWTNPFVLGCLGVGLLTLGGFCLIETRVNQPMFNLRLFRIPAFAFGNV
                     ATLMSSLSRGGLQFMLIIWLQGIWLPLHGYDYSETPLWSAIYMVPLTVGFLGAAPVAG
                     WLSDRFGARMFATGGLLVVAVTFGGLLLLPTDFIYWQFALILFVSGIGAGLFASPNMT
                     GVMNSLPPDQRGAGSGMLSTLQNSGMALSIGVFFSTLIAGLAATMPGALFAGLTQQHV
                     PATVATNVANLPPVGTLFAAFLGYNPMQQILGPQVLAQLPPSNAATVVGKQFFPQLVA
                     DSFHHGLVIVFLTAMAISLIGAIASLFRGGRFVYDEPPRRELDRSDVFSDRSVGPVEF
                     TTPDPRFLARVAERRRGVARR"
     gene            complement(1721..2278)
                     /locus_tag="VGP27_17110"
     CDS             complement(1721..2278)
                     /locus_tag="VGP27_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853052.1"
                     /translation="MDERRTDTREAIRSVALELFAEQGYDKTSLREISERLGVTKAAV
                     YYHFKTKEEILLSLIEDFLAQVDDVVDWAQSQPVTAATRHEVLRRYSELLGGRTTQLA
                     RFMQEGQAAIRDLAAGTEMRKRIDQLVGILATPGHPLPNQLRARVALMALHVGAFAGR
                     EIDATDEQRRDAALQLALEIVGPAS"
BASE COUNT          377 a          856 c          789 g          498 t
ORIGIN      
        1 ccggactcgc cgcgctacaa gtggatcgcg ctgtcgaaca ccaccctcgg cgtgctgatg
       61 gcgacgatga actcctcgat cgtgctcatc tcgctgcccg ccatcttccg cggcatccac
      121 atcgatccgc tgcagccggg caacatcagc tacctcctgt ggatgatcat gggttacatg
      181 ctggtcaccg cggtgctggt cgtcaccctc ggccggctcg gtgacatgta tggacgcgcg
      241 aagatctaca acgccgggtt cgccgtgttc accctggctt ccatcctgct ggcggtggaa
      301 ccgctgggcg gcggcggtgg cgcaatgtgg ctgatcatcg tccgggtcct gcaaggcatt
      361 ggtggcgcga tgctgatggc caacgccacg gcgattctca ccgacgcgtt ctccgccgat
      421 cggcggggcc tcgcagtagg catcaacgcc gtggccgcga tcggcggttc gttcatcggc
      481 ctgctcgtcg gcggcgtgct gtccgaggtc gactggcggc tggtcttctg ggtcagcgtg
      541 cccttcggcg tcatcggcac gatctgggct tacctgagcc tgcacgacac gaacgtccgc
      601 cgatcccaca agatcgactg gccgggcaac atcaccttcg ccatcgggct gatcctgctg
      661 ctggtaggga tcgtctacgt cattcagccc tacggcgggc acgccgaggg atggacgaac
      721 ccgttcgtgc tcggctgtct tggcgtcggc ctgctgaccc tcggcggctt ctgcctcatc
      781 gagactcggg ttaaccagcc gatgttcaac cttcgactgt tccggatccc ggccttcgcc
      841 ttcggcaatg tcgcgaccct gatgagctcg ctgagccgcg gcggcctgca gttcatgctc
      901 atcatctggt tgcagggcat ctggctgcca ctgcacggct acgactattc cgagacgccg
      961 ctgtggtcag cgatctacat ggtcccgctg accgttggat tcctcggcgc cgcaccggtg
     1021 gcgggttggc tgtccgaccg gttcggggcg cgcatgttcg caactggcgg cctgctcgtc
     1081 gtcgccgtca ccttcggcgg actgctgctg ttgccgaccg atttcattta ttggcagttc
     1141 gcgctgatcc tcttcgtcag cggcatcggc gcgggcctgt tcgcgtcgcc gaacatgacc
     1201 ggggtaatga acagcctgcc cccggaccag cgcggggcgg gcagcggcat gctcagcacg
     1261 ctgcagaact ccgggatggc gctgtcgatc ggcgtcttct tctccaccct gatcgccggc
     1321 ctcgctgcca ccatgcccgg ggccctgttc gccggcctga cccagcagca tgtcccggcc
     1381 accgttgcga ccaacgtggc gaacctgcca ccggtcggca ccctgttcgc cgcgttcctg
     1441 ggctacaacc cgatgcagca gatcctcggc ccgcaggtgc tcgcccagct ccccccgtcc
     1501 aacgccgcta cggtcgtcgg aaagcagttc ttcccgcaac tggtcgccga ttcgttccac
     1561 cacggcctcg tgatcgtgtt cctcaccgcg atggccatat ccctgatcgg cgcgatcgcg
     1621 tccctgttcc gaggcggccg cttcgtctac gacgaaccgc ctcgtcgtga actcgacagg
     1681 agcgatgttt tcagcgaccg aagtgtcggt cctgtcgagt tcacgacgcc ggacccacga
     1741 tttctagcgc gagttgcaga gcggcgtcgc ggcgttgctc gtcggtagcg tcgatctcgc
     1801 ggccggcgaa ggcaccgacg tggagcgcca tcagagcgac tctggcgcgg agctggttgg
     1861 gcagcgggtg gcctggggtg gcgagaatac cgacaagctg gtctatccgc ttgcgcatct
     1921 ccgtccccgc agccaggtcg cggatggctg cctggccttc ttgcatgaag cgggcgagct
     1981 gcgtcgtccg cccacccagc agctcgctgt agcggcgcaa cacctcatgg cgggtggccg
     2041 cggtgacagg ttgggattgc gcccagtcga ccacgtcatc gacctgggcg aggaagtcct
     2101 cgatcaggct gagcaggatc tcctctttgg tcttgaagtg gtaatagacc gcggccttgg
     2161 tcacccccag ccgctcggag atctcccgta gcgaggtctt gtcatagccc tgctcggcga
     2221 acaactccag agccaccgac cggatcgcct cccgagtatc ggtacgccgt tcgtccatcg
     2281 tgccagcctc cccgaatctt gccagccgaa cttacttgac gaccggctag taagtgatag
     2341 cgtactcctc ggcagccgct agccggccga caagtaagca gatcgagtga cggaggatca
     2401 gatgaccacg accactgcgg ggccggcccc ggtcgccctg gctgtggacc tgccgcggtc
     2461 caagcgggag atctacacgg tgatgagcgg gctgataatc gccatgctgc tcgcgatgct
//
LOCUS       DASYLX010000731         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1245505,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000731 DASYLX010000000
VERSION     DASYLX010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245505"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..562)
                     /gene="rpsO"
                     /locus_tag="VGP27_17115"
     CDS             complement(293..562)
                     /gene="rpsO"
                     /locus_tag="VGP27_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV7853053.1"
                     /translation="MALTAEQKKDILGSYGLHDSDTGSPEAQVALLTKRIADLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLLKYVAQVDVERYRSLIERLGLRR"
     gene            complement(718..1692)
                     /locus_tag="VGP27_17120"
     CDS             complement(718..1692)
                     /locus_tag="VGP27_17120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV7853054.1"
                     /translation="MQRWRGQDGIPTDWGRCVITIGVFDGVHRGHAELINHAVKAGRS
                     RGVPTVLMTFDPHPMEVVFPGSHPAQLTTLTRRAELVEELGIDVFLVMPFTSDFMKLT
                     PERYVHELLVEHLHAVEVVVGENFTFGKKATGNVDMLRKAGERFGFAVEAMSLVAEHN
                     RDETVTFSSTYIRSCVDAGDVVAAAEALGRPHRVEGVVVRGDGRGKVLGYPTANVAPP
                     MFSAIPADGVYAAWFTVLGHGPVMGTVAPGERYQAAVSVGTNPTFSGRTRTVEAFVLD
                     TEADLYGQHVGVDFVARIRGQERFDSVDDLITAMGGDTEKARTILAAK"
     gene            1774..2478
                     /gene="mntR"
                     /locus_tag="VGP27_17125"
     CDS             1774..2478
                     /gene="mntR"
                     /locus_tag="VGP27_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEV7853055.1"
                     /translation="MNPNGNPRDLTTVAQDYLKVIWTEQEWSLKKVSTKLLAERIGVS
                     ASTASESIRKLADQGLVNHEKYGAVTLTDAGRKAALAMVRRHRLMETFLVRMLGYSWD
                     EVHDEAEVLEHAVSDRMLDRIDAKLGYPKRDPHGDPIPAADGHVPTPDARQLSVCEDG
                     QTGIVARISDADPEMLRYFDSVGITLDSRLMVLARRDFAGMISVAIKSADAKENDPGT
                     TVDLGSPAAEAIWVVG"
     gene            complement(2475..>3617)
                     /locus_tag="VGP27_17130"
     CDS             complement(2475..>3617)
                     /locus_tag="VGP27_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853056.1"
                     /translation="WDWRAVFWVNVPVGVFGTIWAYRRLRDNGERNRGRIDWWGNITF
                     AFGLSAVLIAITTGIQPHGGHSTGWHSPFVVGTLALGVVLLIAFVIIESRVAEPMIRL
                     SLFKIRAFSAGNAATFAAALAQGGLQFMLIIWLQGIWLPLHGYDYADTPLWAGIFMLP
                     LTAGFLVAGPAAGALSDRLGARGIASAGMLLFGLSFVGLMLLPINFPYWAFAMLIALN
                     GIGSGMFAAPNTSAIMGSVLPSERGVVSGMRATFQNSGTALSIGVFFSLMIVGLAGSL
                     PGALSGGLQSHGVPHDVAAQVSELPPVSTLFAAVLGVNPLEHLLAPSGVLTQLPPDSQ
                     AVITGRDFFPSLISGPFHHGLTVVFGASIILAALAALASLARGRVR"
BASE COUNT          672 a         1225 c         1194 g          526 t
ORIGIN      
        1 tcaatacgtc gagcagcctg tcagaccacg ggccttgata cggctacggc cgtcgatcga
       61 agcggccgga ggctcaaccc aatttcttgg ggctcccggc cgccactacc gaagaccgcc
      121 cgatcaggcg gggtccgcca gggacgcgca ggagcggtgc acgcgaactg cgagaaccgc
      181 accgattgtt cgtgccgccc aattggatgt cccgcctaag cggctccaaa cggctgacga
      241 acgcatttat gttacaccgc agcaatgctg cgaccgacat cgtgggattc cgtcagcgac
      301 gcagcccaag gcgctcgatc aaggaacggt agcgctccac gtccacctgg gcgacgtact
      361 tgagcaggcg gcggcgccga ccgaccagca gcagcagtcc gcgccgcgag tggtgatcgt
      421 gcttgtgcac cttgaggtgc tcggtcaggt ccgcgatgcg cttggtcagc agcgcgacct
      481 gcgcctccgg tgaaccggta tcgctgtcgt gcaggccata ggagcccagg atgtcttttt
      541 tctgttcggc agtgagcgcc acgaaacaat ctccatcaat cggtccgcga gggttagggc
      601 tcgaaggccc tgtaaagcgc agccgccgcg aactgcagca cacgcttggt cggcgtgaag
      661 tttagcagcg gagaaggtgc ggcaccgaat cgtggccgcg gccgagccgc gaagacgtca
      721 cttggcggcc agaatggtgc gggccttctc cgtgtcgccg cccatcgccg tgatgaggtc
      781 gtccaccgag tcgaaccgct cctggccgcg gatgcgggcg acgaagtcca ccccgacgtg
      841 ctgaccgtac aggtcggcct cggtgtcgag gacgaacgcc tcgaccgtgc gggtccgccc
      901 ggagaacgtc ggattcgtgc caaccgaaac cgccgcctga taccgctcac ctggcgcaac
      961 ggtgcccatg acgggtccgt ggcccagtac ggtgaaccat gcggcgtaca cgccgtcggc
     1021 ggggatggcc gagaacatcg gcggcgcgac gttggccgtc gggtagccca gcaccttgcc
     1081 gcggccgtcg ccgcggacca ccacgccctc gacccggtgc ggccggccca gcgcctcggc
     1141 cgccgcaacg acgtcgccgg cgtccacgca ggacctgatg taggtggacg agaaggtgac
     1201 ggtttcgtcc ctgttgtgct cggcgaccag ggacatcgcc tcgaccgcga acccgaaccg
     1261 ctcgcctgcc ttgcgcagca tgtcaacgtt gcctgtggcc ttcttgccga aggtgaagtt
     1321 ctccccgacc accacctcga cggcgtgcag gtgctcgacc agcagctcgt gaacgtagcg
     1381 ctcgggcgtc agcttcatga agtccgaggt gaacggcata acgaggaaga cgtcgatgcc
     1441 caactcctcg accagctcgg cgcggcgggt caaggtggtc agctgcgcgg gatggctacc
     1501 agggaaaacg acctccatcg gatgcgggtc gaacgtcatc aacaccgtcg gcacaccgcg
     1561 ggaccggccc gccttgaccg catggttgat cagctcggca tgtccgcggt gaaccccgtc
     1621 gaacacgccg atggtgatga cgcatcgccc ccagtccgtg gggatcccgt cctgaccccg
     1681 ccagcgctgc acagcgccaa gcctacggcg cgccgacggg ccgggatgcc tcacatcccc
     1741 agatcagatg accattgcct aaactttgtc attgtgaacc cgaacggtaa cccgcgcgac
     1801 ctcaccacgg tcgcgcagga ttacctcaag gtcatctgga ccgagcagga atggtcgctg
     1861 aagaaggtga gcacgaaatt gctcgccgag cggatcggtg tgtcggccag cacggcgtcg
     1921 gagtccatcc gcaagctcgc cgaccagggc ctggtgaacc acgagaaata tggcgcggtg
     1981 acgctgacgg acgccggccg gaaggccgcg ctggcgatgg tgcgcaggca ccggctgatg
     2041 gagacgttcc tggttcgcat gctcggctac agctgggacg aggtgcatga cgaggccgag
     2101 gtgctcgagc acgccgtctc cgaccggatg ctggaccgca tcgacgccaa gctcggttac
     2161 ccgaagcggg acccgcacgg ggacccgatc ccggccgccg acggccacgt gccgacgccg
     2221 gatgcgcggc agctgtcggt ctgcgaggac ggccaaaccg ggatcgtggc gcggatatcc
     2281 gacgccgacc cggaaatgct tcggtatttc gacagcgtcg gaatcacctt ggattcccgg
     2341 ctgatggtgc tggctcggcg cgacttcgcg gggatgatct ccgtcgcgat caagtcggcc
     2401 gacgccaagg agaacgaccc gggcaccacc gtggatctgg gcagcccggc cgccgaggcg
     2461 atctgggtgg ttggttagcg aactcgaccc cgagccagcg aggcgagggc ggccaacgcg
     2521 gccaggatga tcgacgcacc gaagaccacc gtcagcccgt gatggaacgg gccggagatg
     2581 aggctcggga agaagtcgcg gccggtgatg accgcctggc tgtccggcgg cagttgcgtc
     2641 agcactccgc tcggcgccag caggtgctcg agcgggttga cgccaagcac cgcggcgaac
     2701 agcgtcgaca ccggcggcaa ctccgatacc tgagcagcga cgtcatgcgg cacaccgtgg
     2761 ctctgtagcc caccgctcag cgcgccgggc aggctgcccg ccaggccgac gatcatcagc
     2821 gagaagaaca cgccgatcga cagcgccgta ccgctgttct ggaacgtcgc ccgcatcccc
     2881 gacaccacgc cgcgttcgct cggcagcacg ctgcccatga tggccgaggt gttcggcgcg
     2941 gcgaacatgc cgctgccgat tccgttgagc gcgatcagca tcgcgaacgc ccagtacggg
     3001 aagttgatcg gcagcagcat caggccgacg aagctcagcc cgaacaacag catgcctgcc
     3061 gaggcgatcc cgcgggcacc gagccggtcc gacagcgcgc ccgccgccgg gccggcgacc
     3121 aggaatccgg ccgtcaaagg aagcatgaag atacccgccc acagcggggt gtcggcgtag
     3181 tcgtagccgt gcagcggcaa ccagatgccc tgcagccaga tgatgagcat gaactgcagc
     3241 ccgccctgcg ccagcgccgc tgcgaatgtc gcggcgttgc ccgcgctgaa cgcccgaatc
     3301 ttgaacaggc tcaaccggat catcggctcc gcgacccggg actcgatgat gacgaacgcg
     3361 atcagcagaa cgacgcccag tgccagcgtt cccacgacga acgggctgtg ccagcccgtg
     3421 ctgtggccac cgtgcggctg gatgccggtg gtgatggcga tgagcaccgc ggacagcccg
     3481 aacgcaaacg tgatgttgcc ccaccaatcg atgcggccgc ggttgcgctc gccgttgtcg
     3541 cgcaggcgtc gataggccca tatcgtgccg aacaccccga cgggcacgtt cacccagaac
     3601 accgcccgcc aatccca
//
LOCUS       DASYLX010000732         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_260248,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000732 DASYLX010000000
VERSION     DASYLX010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260248"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VGP27_17135"
     CDS             complement(<1..984)
                     /locus_tag="VGP27_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7853057.1"
                     /translation="MSDGAIRDDHAELLRRRALTEDAARPDAVDKRHAYGRTARENID
                     DLVDPGSFVEYGRFAIAAQKFRRDLDDLIARTPADGLVAGTARINGELVGDERSACAV
                     LSYDYTVLAGTQGYLGHRKKDRLFELIDRMRLPTVFFAEGGGGRPGDTDYPTVSSLET
                     RAFKLWAQLSGVVPRIAIVKGRCFAGNAVIAGASDLIVATRDASIGMGGPAMIAGGGL
                     GDVHPDEVGPISVQEPNGVVDVVVAGEAEAVAVTKTLIGYFQGPIAPADSGDQTALRT
                     MIPERERRAYDVGPVITTLADEGSVTFLRPRFAREMVTAFARIDGRAVGVIA"
     gene            1080..1574
                     /locus_tag="VGP27_17140"
     CDS             1080..1574
                     /locus_tag="VGP27_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853058.1"
                     /translation="MTVLDERLDPLALERQVCFALAITNRAVLAVYRPLLEPLGLTHP
                     QYLVMLALWDHHRAGRTGRALSVKEIAASLQLDSATLSPMLKRLEVLGLVTRTRSATD
                     ERATDIELTKAGIQLRRKALKIPSAVIERLGVGLDELEHLHTVLTRINGAALAAGALE
                     GERS"
     gene            1571..1981
                     /locus_tag="VGP27_17145"
     CDS             1571..1981
                     /locus_tag="VGP27_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 domain-containing protein"
                     /protein_id="HEV7853059.1"
                     /translation="MNRPKPAQFLGYVFGRRLPDEYRDWVRHDLAGPGAVRRHMIRYA
                     IPPLLVLAPFWLLPASLYVHLEMTAPIYIWSLLITLALNKVWRRHRLAQHGLDPNLVD
                     EINRKKNAHLHDAYVAKYGPRPDEARWQSNSSPF"
     gene            2051..2464
                     /locus_tag="VGP27_17150"
     CDS             2051..2464
                     /locus_tag="VGP27_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV7853060.1"
                     /translation="MTNWINTVSRDHVERGVRGRFTQANHGKPNMLRKMARGDWLVFY
                     SPRTAYPDGDPLQAFTAIGQVADDEPYQVEITDDSHPWRRNVDFLSCTETPIRPLIER
                     LGFIEDKSRWGYKFRFGVFRIDDDDLDVIRSAMTG"
     gene            2480..>2985
                     /locus_tag="VGP27_17155"
     CDS             2480..>2985
                     /locus_tag="VGP27_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7853061.1"
                     /translation="MSNTDASPAEVACAASAFTGVLHPREVPLGGPRAIRVRRTLPQR
                     ERSLVGAWCFADHYGPHDVRDGVGMDVPPHPHTGLQTVSWLFEGEVEHRDSAGVHAFV
                     RPGELNLMTAGAGICHSEVSTAATTVLHGVQLWVALPDSDRDTGRDFAHYAPEPVSVP
                     GAVLRVFLG"
BASE COUNT          476 a         1042 c          969 g          498 t
ORIGIN      
        1 ggcgatcacc ccgaccgccc tcccgtcgat gcgtgcgaat gcggtcacca tctcacgggc
       61 gaaccggggc cgcagaaacg tcaccgaccc ctcgtcggcc agcgtcgtga tcaccgggcc
      121 gacgtcatag gcgcgccgct cacgctccgg aatcattgtg cgcaaggcgg tttggtcgcc
      181 gctgtctgcg ggtgcgatcg gcccctggaa gtacccgatg agcgtcttcg tgacggcgac
      241 cgcttccgct tcgccggcga cgaccacgtc gacgacgccg ttgggctcct gcaccgagat
      301 gggtccgacc tcgtccgggt gcacgtcacc aaggccgcct ccggcgatca tggccgggcc
      361 gcccatcccg atcgatgcgt cacgggtggc gacgatgagg tcggaggcgc ccgcgatgac
      421 cgcgttgccc gcgaagcagc gccccttgac gatcgcgatc cgcggcacca cacccgacag
      481 ctgagcccag agcttgaacg cgcgcgtctc gagagaggac accgtcgggt agtcggtgtc
      541 gcccggcctg ccgccgccgc cctcggcgaa gaagacggtc ggcaagcgca tccggtcgat
      601 caactcgaac agccggtcct tcttgcggtg ccccagatag ccctgcgtcc cggcgagcac
      661 cgtgtagtcg taggacagca ccgcgcatgc gctgcgttcg tcgccgacga gctcgccgtt
      721 gatgcgcgca gtgcctgcca ccagcccgtc tgcgggcgtg cgtgcgatca ggtcgtcgag
      781 gtcgcggcga aatttctgag ccgcgatggc gaaccggccg tactcgacga acgaccctgg
      841 atcgacgagg tcgtcgatgt tctcgcgcgc ggtgcgaccg tatgcgtgcc gcttgtcgac
      901 ggcgtccggc cgggccgcgt cctcggtgag tgcccgccga cgaagcagct cggcgtggtc
      961 gtcacggatc gcaccgtcgc tcatgcggta acgctagccc gcggcctgag cctaacccga
     1021 cgacggttag cctcgtcacc atatggttag tgccctaact attaggttac gatcaagcca
     1081 tgaccgtgct cgatgaacgg ctcgacccgc tggcgctcga acgccaggtc tgcttcgcgc
     1141 tggccatcac caaccgcgca gtgctcgcgg tgtacaggcc gctgttggag ccgctcggtc
     1201 tgacacaccc gcagtatttg gtgatgcttg cgctgtggga ccaccaccgc gccggcagga
     1261 ccgggcgggc gctgtccgtc aaggagatcg cggcgtcgct gcagctggat tcggccactt
     1321 tgtcgccgat gctcaaacgc ctcgaggtgc tcggcctcgt caccaggacg cggtctgcca
     1381 ccgacgagcg ggccaccgac atcgaactca ccaaagcggg tatccaactt cggcgaaagg
     1441 ccctcaagat cccgtccgct gtcatcgagc ggctcggtgt cgggctcgac gaactcgagc
     1501 atctgcacac ggtgctgacc cgaatcaacg gcgctgcgct cgccgcaggc gccctcgaag
     1561 gagaacgctc atgaaccgac ccaaaccggc ccaatttctc ggctatgtgt tcggccggcg
     1621 gctgcccgac gagtaccgcg actgggtacg tcacgatctt gccggtccgg gggccgtccg
     1681 caggcacatg atccgctacg cgatcccacc gcttctcgtg ctggcaccgt tctggctgct
     1741 tccggcgtcg ttgtacgtcc acctggaaat gacggcgccg atctacatct ggtctctgtt
     1801 gatcacgctg gcgctgaaca aggtgtggcg ccgccaccgg ctggcacagc acggtcttga
     1861 tccgaatctc gtggacgaga tcaaccgtaa gaagaacgcc cacctgcacg acgcctacgt
     1921 cgccaaatac ggcccccgcc cggacgaggc tcgctggcag tccaacagca gcccgttcta
     1981 gccgcagggt tgtccacagg ctgttgatcg agcggtcgcg ggtgtcggac ccgcgactta
     2041 gcgtgcgggc atgacgaact ggatcaacac cgtcagccgc gaccacgtcg aacgtggtgt
     2101 ccgcggccga ttcacacagg cgaaccacgg taagccgaac atgctgcgga agatggcccg
     2161 aggcgactgg ctcgtcttct actcccctcg gaccgcctac cccgacggtg acccgctgca
     2221 ggcgttcacc gccattgggc aggtcgccga cgacgagccc tatcaggtgg agatcaccga
     2281 cgactcgcat ccgtggcggc gcaacgtcga cttcctgtcc tgcaccgaga cgcccatccg
     2341 gcccctgatc gagcggctcg gcttcatcga ggacaagagc cgctgggggt acaagttccg
     2401 cttcggggtc ttccggatcg acgacgacga cctcgacgtc atccggtcgg cgatgacggg
     2461 gtagcgatac tgtggccagg tgagcaacac cgacgcctcg cctgccgaag tggcctgcgc
     2521 ggcatcggcg ttcaccggtg tgctgcatcc gcgggaagtt ccgctgggcg gcccgcgggc
     2581 catccgcgtt cgccgcacgt tgccgcagcg ggagcgctcg ctggtcgggg cgtggtgttt
     2641 cgccgaccac tacgggcccc acgatgtgcg cgatggcgtc ggcatggacg tgccgcctca
     2701 tccgcacacc ggcctgcaga cggtcagctg gctgttcgag ggcgaggtcg agcaccgcga
     2761 cagtgcggga gtgcacgcat tcgtccgccc cggcgagctg aacttgatga ccgcgggcgc
     2821 gggcatctgt cattcggagg tctccaccgc ggccacgacg gtcctgcacg gggttcagtt
     2881 gtgggtcgcg ctgcccgact ccgatcgcga caccggccgc gacttcgccc actacgcacc
     2941 cgaaccggtg tccgtgcccg gcgccgtctt gcgggtgttc ctcgg
//
LOCUS       DASYLX010000733         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_918285,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000733 DASYLX010000000
VERSION     DASYLX010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918285"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VGP27_17160"
     CDS             <1..1175
                     /locus_tag="VGP27_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7853062.1"
                     /translation="ALMQLTSGSTGSPKAVQISHRNFVANAEAMFTGAEVDPDTDVIV
                     SWLPCFHDMGMTGFLAVPMYFGVELVKVTPMDFLRDTLLWAKLIDRYKGTMTAAPNFA
                     YILFAKRLRNQASPGQFDLSSLRWALSGAEQVDPADVAELCEAGAPFGLRPEAILPAY
                     GMAETTVAVSFTQCGKGLTVDEVDAHLLAVLRRAVPATHGRTRRFASLGRLLKGLEAR
                     VVDVDGAVLPARGVGVIEVRGEPVTLGYTTAAGFVSAQDDDGWYDTGDLGYLTEAGDV
                     VVCGRLKDVIIMAGRNIYPTDIERAACRVEGVRPGCAVAVRLDAGRSRESFAVAVESN
                     SYDDHEAVLRIQHQVAHEVVAEVNVRPRNVVVLEPGIIPKTPSGKLRRAHALALVD"
     gene            1272..2258
                     /locus_tag="VGP27_17165"
     CDS             1272..2258
                     /locus_tag="VGP27_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV7853063.1"
                     /translation="MTDVRGRVWRGGKAQDDFDFSSISDYLVEDDTLVWCDIFDPDES
                     TLSDLAQELGLNNWAVEDAIGEAERTKAVVYRSHTFFTVYAVSGREPADDSDSTLTTH
                     RISGFVLPQGLITVRLSPWYDIETVSRRFDELGGQEYGVGALVHGLLDVVVDGHFEVV
                     QALDDGIEGLEDDLFENNTPRGGLQRKTFRLRKDLVELRRVVLPMREVVNSIQHRRLD
                     AKTAPELDPLYADLYDHVLRVSEWTESLRDMVTTVFETNLSLQDARLNQVMKKLTGWA
                     AIIAVPTAVTGFYGQNVEYPGIDTVGGFITSTAVIVVLVLLLYWVFKRRDWL"
     gene            complement(2385..>3225)
                     /locus_tag="VGP27_17170"
     CDS             complement(2385..>3225)
                     /locus_tag="VGP27_17170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEV7853064.1"
                     /translation="VLASGATPVPLAVPGGEAALSLRSFADAVVLRMAARNSDSAVVV
                     GGGLIGCEAASSLAVRGVATTLVAPEPVPVHRRFGFDAGERVAKMLSNIGVRFIGGTA
                     VAAVEDRGVTLDSGATVKADLVVSATGVRPDSRLATAAGLVTEGGRVLADERMHTSAP
                     NVYAAGDVALAYNVTAGRRVASEHWHDATEQGRIAGTVAAGYSDSWKTVPEFSCQIGE
                     SVLKYRGWGDGCDLTRLVDRRDGFTAWYGRGGEIAGVLSLNSDDDYETGGRLLRSHAP
                     IPG"
BASE COUNT          548 a         1024 c         1088 g          565 t
ORIGIN      
        1 tggcgctgat gcagttgacg tccgggtcaa cgggctctcc caaggcggtc cagatcagcc
       61 atcgcaactt cgtggccaat gcggaggcga tgttcaccgg ggcggaagtc gacccggata
      121 ccgatgtgat cgtcagctgg ctgccatgct tccacgatat gggtatgacc ggtttcctcg
      181 cggtgccaat gtatttcggg gttgaactgg tcaaggtcac cccgatggac tttctgcgtg
      241 acaccttgtt gtgggccaag ttgatcgacc gatacaaggg cacgatgaca gccgccccca
      301 acttcgccta catcctcttc gccaagcgac tgcgcaatca agcgtccccg gggcagttcg
      361 acctgtcctc attgcggtgg gcgctgtcgg gggctgaaca ggtcgacccg gccgacgtgg
      421 ccgagctgtg cgaggcgggc gcgccattcg ggttgcgccc tgaggccatt ctgcccgcct
      481 acggcatggc agagacgacg gtggcggtgt cgttcaccca gtgcggcaaa ggccttaccg
      541 tcgacgaggt ggatgcccat ctgctcgcgg tgttacgccg ggctgtcccg gccacccacg
      601 gccgcacccg acggttcgcc tcactcggcc ggctgttgaa gggtctcgaa gctcgcgtcg
      661 tcgatgtgga cggcgccgtg ctgccggcac gcggcgtcgg tgtgatcgag gtgcgcggtg
      721 aacccgtcac gctcggatat accaccgcgg cgggattcgt gtcggcacag gacgacgacg
      781 gttggtacga cacgggcgat ctcggctacc tcaccgaggc gggcgatgtc gtggtgtgcg
      841 gtcgcctcaa ggacgtcatc atcatggcgg gccgcaacat ctatcccacc gacatcgagc
      901 gggccgcctg ccgagtcgag ggtgtgcggc cgggctgtgc cgtggcggtg cggctggacg
      961 ccggacgctc acgcgaatcc ttcgcagtgg ccgtggaatc caactcttac gatgatcacg
     1021 aagcggtgct tcgcatccag caccaggtcg cgcacgaggt ggttgccgag gtcaatgtcc
     1081 ggccccgcaa tgtcgtcgtg ctcgaaccgg ggatcattcc caagacgccg tcgggaaaac
     1141 tgcggcgcgc acatgcactg gcgctcgtcg actgattgcc cccaccggag aacgaccgcg
     1201 tgaggtccat tgccatctgc gatggacgga gccacaacaa ccacgtcatc ccgctgggag
     1261 atgatggtgg catgaccgat gtacggggcc gggtgtggcg gggcggcaag gctcaggacg
     1321 acttcgactt ctcctcgatc tccgactatc tcgtcgagga cgacaccctg gtgtggtgtg
     1381 acatcttcga cccggacgag tcgacgctgt cggatctcgc acaggaactc gggttgaaca
     1441 actgggccgt cgaggacgcc atcggtgaag ccgagcgcac caaggccgtc gtgtaccgtt
     1501 cgcacacctt cttcaccgtg tatgcggtct cgggcaggga acccgccgac gactccgact
     1561 cgacactgac aacccaccgg atctccgggt tcgtgctgcc gcagggcctc atcaccgtgc
     1621 ggttgtcacc gtggtacgac atcgagaccg tgtcgcggcg cttcgacgaa ctcggcggcc
     1681 aggagtacgg ggtcggcgcg ctcgtgcacg gcctgctcga cgtcgtcgtc gacggccact
     1741 tcgaggtggt gcaggcgctc gacgacggta tcgagggact cgaggacgac ctgttcgaga
     1801 acaacacgcc gcggggtgga ctgcagcgca agacttttcg gctgcgcaag gacctggtcg
     1861 agctgcgccg ggtggtgctg ccgatgcgcg aggtcgtgaa ctcgattcag caccgcaggc
     1921 tggacgcgaa gacagcgcct gaactggacc cgctatacgc cgacctgtac gaccacgtgc
     1981 tgcgggtgtc ggagtggacg gagtccttgc gggacatggt gacaacggtg ttcgagacga
     2041 atttgtcgtt acaggacgcc cggctgaacc aggtgatgaa gaaactgacg ggttgggcgg
     2101 cgatcatcgc ggtgccaacg gcggtcaccg ggttctacgg gcagaacgtg gagtacccgg
     2161 gcattgacac ggtcggcggc ttcataacga gcacggcggt gatcgttgtg cttgtgctgc
     2221 tgctctattg ggtgttcaag cgtcgcgact ggctgtagcc ctgtggcggc ggcgtgggcg
     2281 gcggaaatgt cgcatgtctg cccctttggt cgacggaccg tttcgatccg aagccggagt
     2341 tgcccttctc cacatcgaca aacgaccgtc gtcggcgaac gcggtcaacc agggatgggt
     2401 gcgtgcgacc ggagcagtcg cccgcccgtc tcgtagtcat cgtctgagtt gaggctcagg
     2461 acgccggcga tttcaccgcc gcgtccatac caagccgtga acccgtcgcg gcggtcgacg
     2521 agcctggtga ggtcacatcc gtcaccccag ccccggtatt tgaggacgga ttcgcctatc
     2581 tggcaggaga attcgggaac tgtcttccag gagtccgaat aaccggcggc tacggtgccc
     2641 gcgatacgcc cttgctctgt ggcgtcgtgc cagtgttcgg acgccactcg ccgtcccgcg
     2701 gtgacgttgt aggcaagggc gacgtcccct gcggcgtaga cgttcggcgc cgaggtgtgc
     2761 atgcgctcgt cggcgaggac ccggccgcct tcggtgacca gaccagccgc ggtggcgagc
     2821 ctgctgtcgg gtcgtactcc ggtagcggat acgacgaggt cggccttcac cgtcgcaccg
     2881 ctgtccagag tgactccacg gtcctcgact gcggccacag ccgtcccacc gatgaatcga
     2941 acaccgatgt tggacaacat cttcgcgacg cgctcaccgg catcgaaacc gaacctgcgg
     3001 tgcacgggca ccggctcggg cgcgacgagc gtggtcgcga caccgcggac cgcgaggctg
     3061 gacgccgcct cacaaccgat caggccgccg ccgaccacga ccgccgaatc ggaatttctg
     3121 gcggccatcc gaaggacgac ggcgtcggcg aacgaccgca gggacaacgc cgcctcgccg
     3181 ccgggaaccg ccagcgggac cggtgtcgcg ccggaggcca gcacc
//
LOCUS       DASYLX010000734         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2893246,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000734 DASYLX010000000
VERSION     DASYLX010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893246"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VGP27_17175"
     CDS             <1..1188
                     /locus_tag="VGP27_17175"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853065.1"
                     /translation="VVLASGPLPDHKLPDIRGVDTYAGKMIHSARWDHDYDFTGKRVA
                     VVGTGASAVQIIPELVKQADFVKVFQRTPGWVLPRLDVPMPAAAQGLFAKVPATQQLA
                     RQALYWGHEITATALVWDSPLTGFVARLSRAHLRRQVKDPWMRRQLTPDFTPGCKRML
                     VSSDYYPALQRENCKLIDWPIATLSPVGIRTSDGVEHHVDCIVFATGFDVHLTGPPYP
                     VTGIGGRSLAEEWADGAQAYKSINAHGYPNLYFMTGPNSGPGHNSLLVYVEGQIDYAV
                     RGITTIVGDGLHYLDVREDVQRRYNRRMQKRLGKTTWMSGCSSWYLTKDGFNASMYPG
                     FATQYLRQMRDFRFGDYIAAAHARSKSSTAHARSKSSIAHARSKSSTAHDVTTPPAVT
                     STA"
     gene            1201..>1647
                     /locus_tag="VGP27_17180"
     CDS             1201..>1647
                     /locus_tag="VGP27_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7853066.1"
                     /translation="MSGARQPRQPPGAISTILGGLRRAPRQIRRGSRDVIETAVSQLF
                     DAAVQPHGVAASGEYRIEELARIAGTTIRNIRVYRDRGLLHPPLRVGRIALFNDTHLT
                     RLRLITSLLDRGYNISHVHEMLSAWEQGKNVGDMLGLESAIAGSWAT"
BASE COUNT          306 a          567 c          523 g          251 t
ORIGIN      
        1 gtggtgttgg cctccggacc actgcccgat cacaagctgc ccgacatccg aggcgtcgac
       61 acctacgcgg gcaagatgat tcacagcgcg cgctgggacc acgactacga cttcaccggc
      121 aagcgtgtcg ccgtcgtcgg aacgggtgcc agcgccgtgc agatcatccc tgaactggtc
      181 aagcaggcgg acttcgtcaa ggtcttccaa cgcaccccgg gctgggtgct gccgcggctg
      241 gacgtgccga tgcccgcagc cgcacaaggg ctgttcgcga aagtgcctgc cacacaacag
      301 ctcgcccgcc aggcgctgta ctggggacat gagatcaccg ccaccgcgct ggtttgggac
      361 agtccgctga ccggtttcgt cgcccggctg agcagggcgc acctgcgccg tcaggtgaag
      421 gacccctgga tgcgcaggca actcacgccg gacttcaccc ccggctgcaa gcggatgctg
      481 gtctccagtg actactaccc cgccctgcag cgcgaaaact gcaaactgat cgactggccc
      541 atcgcgaccc tgagccctgt cggcatccgg accagcgacg gcgtcgagca ccacgtcgac
      601 tgcattgtct tcgccaccgg gttcgacgtg catctgacgg gaccgccgta tccggtcacc
      661 ggcatcggcg ggcgctcgct tgccgaggag tgggccgacg gtgcccaggc gtacaagagc
      721 atcaacgcgc acggttatcc gaacctgtac ttcatgacgg gaccgaactc cggacctggc
      781 cacaactcgt tgctggtgta tgtggaaggg cagatcgact acgcggtgcg cggcatcacc
      841 acgatcgtcg gcgacggtct gcactatctc gatgtccgcg aggacgtgca gcgacgctac
      901 aacaggcgca tgcagaagcg gctcggcaag acgacctgga tgtcgggctg cagcagttgg
      961 tatctgacga aggacggctt caacgcatcg atgtacccgg gcttcgccac gcagtatctt
     1021 cgtcagatgc gggacttccg attcggcgac tacatcgcgg ccgcgcacgc gaggagcaaa
     1081 agcagtaccg cgcacgcgag gagcaaaagc agtatcgcgc acgcgaggag caaaagcagc
     1141 accgcgcacg acgtgaccac cccgcctgcg gtcacctcca cggcctgaga tgccctagag
     1201 atgtccgggg ccaggcagcc acgacaaccg ccgggggcga tctcgaccat cctcggcggt
     1261 ctgcgccgcg cgccccgcca gatccgcagg ggctcccgcg atgtcatcga gaccgcggtg
     1321 tcgcagctgt tcgacgccgc cgtgcaaccc cacggcgtcg cggcctccgg cgagtaccgg
     1381 atcgaggaac tcgcgcggat cgccggcacc accatccgca acatccgcgt ataccgcgac
     1441 cgcggcctgc tgcatccgcc gctgcgggtg gggcgcatcg ccttgttcaa cgacacacac
     1501 ctgacgcggt tgcggctgat cacctctttg ctcgaccgcg gctacaacat ctcgcacgtg
     1561 cacgagatgt tgtcggcgtg ggagcagggc aaaaacgtcg gcgacatgct cggcctggag
     1621 tcggcgatcg cgggcagctg ggccacc
//
LOCUS       DASYLX010000735         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_40000, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000735 DASYLX010000000
VERSION     DASYLX010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40000"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..738)
                     /locus_tag="VGP27_17185"
     CDS             complement(154..738)
                     /locus_tag="VGP27_17185"
                     /inference="COORDINATES: protein motif:HMM:NF042716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6611 family protein"
                     /protein_id="HEV7853067.1"
                     /translation="MGQRKPDGMQEMMENRPGRLQSWCARLLDGENRWGFVRIQVDRF
                     GVTRYRLVVYPPGISDDQRRRLRIWRGAPIWGAVLWFVAEVFLQRVIGPWATLATSTG
                     TVIALVAVALMRADETRARVRAIGVVTMAGHKDVATVAARDNLLAKAMTLTYADERVD
                     EERMSPLDHEALWWQIYEQLAPAGSVASERKMPQ"
     gene            complement(788..913)
                     /locus_tag="VGP27_17190"
     CDS             complement(788..913)
                     /locus_tag="VGP27_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853068.1"
                     /translation="MVRMGCCILRTQELLRSIGQGDHFLTRAPILPAWTVSAVRR"
     gene            1024..1221
                     /locus_tag="VGP27_17195"
     CDS             1024..1221
                     /locus_tag="VGP27_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853069.1"
                     /translation="MTAAALRTFGDIDDTSRPLAVLVQCARAVLDVGPGSGVEILDGV
                     SHFLHLEQRLIPEKITARSAG"
     gene            complement(1218..1781)
                     /locus_tag="VGP27_17200"
     CDS             complement(1218..1781)
                     /locus_tag="VGP27_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853070.1"
                     /translation="MAARSSPDAELSTRRRILAATFVVLARDGRRKLQLSDVAAEAKV
                     SRPTLYRYFGSKEGLLEAFGLYEQDNFDSGIAAAMAGLSGAVRLDASAALHRGVSEHV
                     LTGFARDIEPEHVLNQMKRVLPIMHERIARIISDDENEITASAVVRITMCHYMIRSGS
                     PDQFLAELRHTAGLGTSRRRMKRAAAG"
     gene            1918..2205
                     /locus_tag="VGP27_17205"
     CDS             1918..2205
                     /locus_tag="VGP27_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853071.1"
                     /translation="MTKFMVDKIPWDFDASVPIMWQPANPGLGLFCNTFTSIAAPFER
                     CDENARELHQLRDLLVRVEDPVGGGTGLGNAETFALGAFPERGLTAENSFL"
     gene            2202..>2625
                     /locus_tag="VGP27_17210"
     CDS             2202..>2625
                     /locus_tag="VGP27_17210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853072.1"
                     /translation="MTVAVIGAGPGGIAMGIQLAAAGHDFTIFDRADGFGGTWRNNTY
                     PGAACDVPSHFYSYSFALNPRWSKTFAQQPEILAYLEEVASRYGLDGHFVGNTRVTEL
                     RWDDDAQKWSIETAEGATYEFDAVVSAVGMLDVPSIPKI"
BASE COUNT          465 a          873 c          834 g          453 t
ORIGIN      
        1 atgtcgtggt cctgggcccc ggcaccattc ccaagacgcc gtcgggcaag ctgcggcgcg
       61 caaactccgt ctcgctggtc acctagcccc gcgagcgagc tcgctggtca cctcaccccg
      121 cgagcgtgcg tgtctgtacc ccgacacgcc aagctactgc ggcattttgc gctcgctcgc
      181 gacggatcca gcgggcgcta actgctcgta gatctgccac cagagcgcct cgtgatcgag
      241 tggcgacatg cgctcctcgt cgacgcgctc gtcggcgtac gtcagcgtca tggccttcgc
      301 cagcagattg tcacgagcgg caacggtagc cacatcttta tggcctgcca tcgtcaccac
      361 accgatggcg cggacccgcg cgcgtgtttc gtcggcacgc atcagcgcga ccgcgaccag
      421 cgcgatgacc gtgcccgtgg aagtcgccag cgtggcccac ggtccgatca cccgctgtag
      481 gaagacctcc gccacgaacc acagcaccgc gccccagatc ggcgcgccac gccagatgcg
      541 gagccgacga cgctggtcat cgctgatgcc gggcggatag accaccagcc ggtagcgcgt
      601 cacgccgaag cggtcgacct ggatgcgaac gaaaccccac cggttctcgc cgtcgagcag
      661 ccgggcgcac cagctctgca gtcgaccggg gcggttttcc atcatctctt gcataccgtc
      721 gggtttacgc tggcccacct gctaaacgtg ttatccgaac gcttccttga cggtccgcga
      781 cgggttctta acgccggacg gcggagaccg tccatgcggg caggatcggc gctcgggtga
      841 ggaagtggtc gccctggccg atgctgcgca gcaattcctg cgtacggaga atgcagcagc
      901 ccatccgaac catggcgaac acctcgcagt cgaagcccgg cagccgatca aatatgcata
      961 cgcacctcca catgtaaagc cattatgtgt acgtaccgcg gtttataggt atacgcccgc
     1021 gaggtgaccg cggccgcact gcgcaccttc ggcgacatcg acgacacgtc tcgtccgctg
     1081 gccgttctcg tacagtgtgc tcgcgccgta ctcgacgtag ggccaggctc cggcgtcgaa
     1141 atcctcgacg gcgtaagcca tttcctgcat ctcgaacagc ggctgatccc ggaaaagatc
     1201 accgcccggt cggcaggcta gccggccgcg gcgcgtttca tgcgccgtcg agacgtcccc
     1261 aggcccgcgg tgtggcgcag ttcggcaaga aactgatcgg ggctgccgct gcgaatcata
     1321 tagtggcaca tagtgatacg gacgaccgcc gaggccgtga tctcgttctc gtcgtcggaa
     1381 atgatgcggg cgatgcgctc gtgcatgatc gggagaaccc gcttcatctg gtttaggaca
     1441 tgctcgggtt cgatgtcgcg agcgaaccca gtgagtacgt gctctgaaac tccacgatga
     1501 agcgcagcgc tggcgtccag gcgaacggcg ccgctcaatc ctgccatcgc cgcggcgatt
     1561 ccagagtcga agttgtcctg ctcgtagagg ccgaacgcct cgagcagtcc ctctttggaa
     1621 ccgaagtacc ggtacagcgt cgggcgcgag accttggcct cggcggcgac gtcggacagt
     1681 tgcagcttgc gtcgcccgtc tcgagccagc accacgaatg tggctgcgag gattcggcgc
     1741 cgcgtcgaca gttcggcgtc gggggacgag cgggctgcca caatcgcacc ctttacatct
     1801 aggggttcga atgtcacgct agcagctgcc gggcggctgt gtgcctgcat gacagtgcga
     1861 gacagatgtg cgcagaatgg taagccagcg acggccatcg actgggaggt ggcgacgatg
     1921 acgaagttca tggtcgacaa gatcccgtgg gacttcgacg cgtcggtgcc gatcatgtgg
     1981 cagccggcca accccggact cgggctgttc tgcaacacct tcacgtccat cgcggcgccg
     2041 ttcgaacgct gcgacgagaa cgcccgcgaa ctgcatcagc tgcgtgatct gcttgtgcgc
     2101 gtcgaggatc ccgtcggcgg cggcacgggt ctgggcaatg ccgagacgtt cgcgctcggc
     2161 gccttccccg aacgcgggct gaccgcggag aactcgttcc tgtgacggtc gcggtcatcg
     2221 gagccggccc cggtggcatc gcgatgggca tccagctcgc ggcggcaggc cacgacttca
     2281 cgatcttcga ccgcgccgac gggttcggcg gcacctggcg caacaacacc tatcccggcg
     2341 cggcatgcga cgtgccgtcg cacttctact cgtactcgtt cgccctcaac ccgcggtgga
     2401 gcaagacgtt cgcccagcag ccggagatcc tcgcgtatct ggaggaggtg gctagccgct
     2461 acgggctcga tgggcatttc gtcggcaata cccgagtcac cgagctgcgc tgggatgacg
     2521 acgcacagaa gtggtcgatc gaaaccgccg agggcgcgac ctacgagttc gacgccgtcg
     2581 tgagcgcggt cggaatgctc gacgtgccca gtatccccaa gattc
//
LOCUS       DASYLX010000736         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1904920,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000736 DASYLX010000000
VERSION     DASYLX010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904920"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..537
                     /locus_tag="VGP27_17215"
     CDS             157..537
                     /locus_tag="VGP27_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853073.1"
                     /translation="MKSIAVLALACGVTAAPMMAAGTAWADRGACDGVDCVPFVDRNI
                     VPTDYCSFATRYVYGVDAKGDTFACAATNQWVPVAPLIGVRTLRAPCDPNVPASAQSG
                     AGQPLNCEAGAFTSDFKTLYYAGS"
     gene            complement(573..785)
                     /locus_tag="VGP27_17220"
     CDS             complement(573..785)
                     /locus_tag="VGP27_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853074.1"
                     /translation="MGVASADPPPPPNPPWAAVGDPVPSWAPGKPAEFWLGEPVVWTT
                     GWGGRWCVWKNGQIITLSSNPVTNGG"
     gene            complement(848..1291)
                     /locus_tag="VGP27_17225"
     CDS             complement(848..1291)
                     /locus_tag="VGP27_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5078 domain-containing protein"
                     /protein_id="HEV7853075.1"
                     /translation="MLGKASIVAAAVSMVAMTIPAVASADATDDYPIPNRILKTTCTV
                     DQYMAATRDTDPVYYERYMIDYHNRPIDVQDAARDRIYWFFTLDYAGRRQYSEDTATN
                     VYYEQMATHWGNWAKLFFNNKGVAAHATDVCMNYPPNDPTVWDWT"
     gene            complement(1312..1713)
                     /locus_tag="VGP27_17230"
     CDS             complement(1312..1713)
                     /locus_tag="VGP27_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7853076.1"
                     /translation="MRGKQISSAVLTALALTVPALAVAAPAHSDPDTDFYNELHIYGI
                     YGEKDYNAWIGKIACKRLWNNLDHDANQSAQFVQRQLKKDTTTEQTYQFLGAAYRTYC
                     PDKLPILTQTAQPGPGAGTPPAPAGPPLAAE"
     gene            complement(1773..>3971)
                     /locus_tag="VGP27_17235"
     CDS             complement(1773..>3971)
                     /locus_tag="VGP27_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853077.1"
                     /translation="ATLGYYELIGLSTFAVNLLTTLAIAAGTDYAIFLVGRYHEARNN
                     GEDRETAFYTMYHGTAHVILGSGLTIAGATFCLHFTRLPYFQTLGIPLAIGMLVAVAA
                     ALTMAPALLTICSRFGLFDPKRRMQERGWRKIGAAVVRWPGAILVSTIALSLVGLLAL
                     PGYVPSYNDREFLPADIPANQGYQAADRHFPQARMNPEMLLIETNHDVRNSADMLVVD
                     RVAKAIFHIPGIGRVQTITRPLGTPIEHTSIPFQISMQGTTQQMNQDYMDKVMSNMLI
                     QANDMQTTIDTMTRMQSLTQQMADTTHAMVEKTKQMAVDIGEVRDNIADFDDFLRPIR
                     NFLYWEPHCYDIPVCHSIRAIFDTLDGIDVLTDDVQTLVPLLERLDTLMPQLVAIMPS
                     MIETMKNMKNITLTMYQSQKGQQDQTKALQENSTAMGQAFDASKNDDSFYLPPEVFNN
                     ADFKRGMKMFVSPDGKAVRFIISHEGDPATPEGISHIDQIRNAAFEAIKGTPLEGSKI
                     YLAGTASTYKDMAAGSNFDLLIAGIASLCLIFIIMLLLTRAVVAAAVIVGTVLLSLGT
                     SFGLSVLIWQYILGMHLHWMIIAMSVIILLAVGSDYNLLLVSRFKEEIHAGINTGIIR
                     SMGGTGSVVTSAGLVFAFTMMSMVVSDLRVIGQVGTTIGLGLLFDTLIIRSFMTPAIA
                     ALLGKWFWWPQRVRQRPVPEPWPEPKTEQDQVGVTPGRHSNDESPTGPIR"
BASE COUNT          719 a         1198 c         1267 g          787 t
ORIGIN      
        1 ccgaaacgcg agcgcgcgga tctgttcaag gcccgggcga ccgccgaggg cctgcgggtc
       61 gggtcgacgt gacgcaaagg cacgagaaga ccttatgccg tggcaacgcc ccggtacgct
      121 aaccgtcgaa gccggtccac agcggagggg acgaacatga agtcgatcgc ggtactggca
      181 ctggcctgcg gcgtgaccgc cgcaccgatg atggcggcgg gtactgcctg ggcggatcgc
      241 ggcgcctgcg acggcgtgga ctgcgttccc ttcgtggatc gcaacatcgt gcccaccgac
      301 tactgctcgt tcgcaacccg gtatgtgtat ggggtggatg cgaagggcga cacgttcgcc
      361 tgtgccgcga cgaaccagtg ggtgccggtg gctccgctga tcggagttcg caccctacgg
      421 gcaccgtgcg acccgaatgt gccggcttcg gcgcagtcag gcgccggtca gcccctcaat
      481 tgcgaggccg gagcgttcac gtccgacttc aaaacgctct attacgccgg cagttgaggg
      541 cccgcggcgc caggcggtaa cgagggaaag ggttaaccgc cgtttgtgac ggggttcgac
      601 gacagcgtga tgatctgccc gttcttccag acgcaccacc gtccgcccca accggtggtc
      661 cacaccacgg gctcacccag ccagaactcc gccggcttgc ccggcgccca ggacggcacc
      721 gggtcgccca cggcagccca cggcggattc ggcggcgggg gcgggtctgc gcttgccacg
      781 cccatgccga gaacgcccgc ggccagtccg catgcgaccg ccagcacagg tagggccctg
      841 ctgaaggtca cgtccagtcc cacactgtcg gatcgttcgg cgggtagttc atgcagacgt
      901 cggtggcatg tgcggcgaca cccttgttgt tgaagaacag cttcgcccag ttgccccagt
      961 gcgtggccat ctgctcgtag tagacgttgg tcgcggtgtc ttccgagtac tgccgcctgc
     1021 ccgcgtaatc cagcgtgaag aaccagtaga tgcggtcgcg ggcagcgtcc tgcacgtcga
     1081 tcggcctgtt gtggtaatcg atcatgtagc gctcgtagta caccggatcg gtgtcgcgcg
     1141 ttgcggccat gtactggtcg accgtgcagg tggtcttgag tatccggttc gggatcggat
     1201 agtcgtctgt cgcgtcggcc gatgcgacgg ctggaatcgt catcgccacc atgctcaccg
     1261 cggccgccac gatgctcgcc ttacccagca tcacttctcc ctttcccttt attactctgc
     1321 cgccaacggc ggacccgccg gtgcaggcgg cgtacccgca cccggacccg gttgcgccgt
     1381 ttgtgtgagg atcggcagct tgtcggggca gtaggtccga taggcggcac cgaggaactg
     1441 atatgtctgc tcggtggtgg tgtccttttt cagctgccgc tggacgaatt gggcggactg
     1501 attggcatcg tgatcgaggt tattccacag tcgcttacac gcgatcttgc cgatccacgc
     1561 gttgtaatcc ttttcaccgt agatcccgta gatgtgcagc tcgttgtaga aatcggtgtc
     1621 ggggtcggaa tgtgctggtg ctgccacagc cagcgcgggc accgtcaacg cgagggccgt
     1681 caatacggcc gaactaatct gtttccccct catgggcacc tatcttatct gattgacttt
     1741 tcaaattaac cccatcgggc tttcttcgtt ctctatcgaa ttggccccgt ggggctttca
     1801 tcgttcgaat ggcggccggg cgttacaccg acctgatctt gttctgtttt cggctccggc
     1861 cacggctcgg ggacaggacg ctggcggacc cgttgcggcc accagaacca tttaccgagc
     1921 agcgcggcga tcgccggcgt catgaacgac cggatgatca gcgtgtcgaa caacaggccg
     1981 agaccgatgg tggtgcccac ctgaccgatc acccggagat cgctgaccac catcgacatc
     2041 atggtgaagg cgaacaccag cccagccgaa gtgacgaccg agccggttcc acccatcgac
     2101 cggattatgc ccgtgttgat gcccgcatgg atctcttcct tgaaccggga gaccagcagc
     2161 aggttgtagt cggatcccac cgccaacagg atgatgaccg acatcgcgat gatcatccaa
     2221 tgcagatgca tgccgaggat gtactgccag atcagcaccg aaaggccgaa cgatgtgccc
     2281 agcgacagca gcaccgtacc gacgatcacc gcggcggcca ccaccgcacg ggtcagcaac
     2341 agcatgatga tgaaaatcag gcacagcgaa gcgattccgg cgatcagcag gtcgaaattc
     2401 gatcccgccg ccatgtcctt atatgtggat gcggtgcctg ccaggtagat cttcgagccc
     2461 tccagcggcg tgcccttgat ggcttcgaac gccgcgttcc tgatctgatc gatgtgcgaa
     2521 atgccctctg gcgtagcggg atcaccttca tgcgagatga tgaacctgac cgccttgccg
     2581 tccggcgaga cgaacatctt catcccgcgc ttgaagtcgg cgttgttgaa cacctcgggt
     2641 ggcaggtaga acgagtcatc gttcttggac gcgtcgaacg cctggcccat cgcagtcgag
     2701 ttctcctgca gggccttcgt ctgatcctgc tgacccttct ggctctggta catggtcagc
     2761 gtgatgttct tcatgttctt catcgtctcg atcatcgacg gcatgatcgc aaccagttgt
     2821 ggcatcaggg tgtccagacg ctccaacagg ggcaccagcg tctggacgtc atcggtcagc
     2881 acatcgatgc cgtcgagcgt gtcgaagatg gcccgtatcg aatggcagac ggggatgtcg
     2941 tagcagtgcg gttcccagta cagaaagtta cgaatggggc gaaggaagtc gtcgaagtcg
     3001 gcgatgttgt cccggacttc gccgatatcg acggccatct gcttggtctt ctcgaccatg
     3061 gcgtgggtgg tgtcggccat ctgctgggtc aggctctgca tacgtgtcat cgtgtcgatg
     3121 gtcgtttgca tgtcgttggc ctggatcagc atgttcgaca tgaccttgtc catgtagtcc
     3181 tggttcatct gctgggtggt gccctgcatg ctgatctgga acggaatgga cgtgtgttcg
     3241 atcggtgtgc cgagcggccg ggtgatggtc tgcacgcgac cgataccggg aatgtggaag
     3301 atggctttgg caactcgatc aacgacgagc atgtcggctg aattgcgcac atcgtggttg
     3361 gtttcgatga gcagcatctc cgggttcatc cgggcctgcg ggaaatggcg gtctgcggcc
     3421 tgatagccct gattggcggg gatgtcagcc ggtaggaact cgcgatcgtt atagctcggc
     3481 acgtagccag ggagcgcaag caggccgacg agtgaaagcg caatggtgga caccagaatc
     3541 gctccgggcc accgcaccac cgcggcaccg atcttgcgcc agccgcgctc ctgcatcctg
     3601 cgcttcgggt cgaagagccc gaagcgactg cagatggtca gcagtgcggg cgccatggtc
     3661 agcgcggccg cgaccgcgac gagcatgccg atggccaacg gaatgcccag cgtctggaag
     3721 tacggcagcc tggtgaagtg caggcagaag gtggcgcccg cgatggtcag gcctgagcca
     3781 aggatgacgt gcgccgtgcc gtgatacatg gtgtagaacg cggtttcgcg gtcctcaccg
     3841 ttgttacggg cctcgtgata tcggccgacg aggaagatcg cgtagtccgt ccccgccgcg
     3901 atcgccaacg tggtgagcag gttgacggcg aaggtcgaca gaccgatcag ctcgtaatag
     3961 ccgagcgtcg c
//
LOCUS       DASYLX010000737         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1686227,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000737 DASYLX010000000
VERSION     DASYLX010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686227"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGP27_17240"
     CDS             complement(<1..358)
                     /locus_tag="VGP27_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-4DMT family transporter"
                     /protein_id="HEV7853078.1"
                     /translation="MSKWLVRGLVFAALMVFIRLVQGAAINAWETKAGLISITLVVIF
                     TIIVFVWGFLDGRSDARTNPDPDRREDLSMTWLLAGLFAGLVSGAVAWLISLFYKGLY
                     VGGLINELTTFAAFTAL"
     gene            392..1507
                     /locus_tag="VGP27_17245"
     CDS             392..1507
                     /locus_tag="VGP27_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV7853079.1"
                     /translation="MTISQRRDRLRKRLESDGLAAMLVTRLVNVRYLSGFTGSNAALL
                     IRADDDTPVLATDGRYRTQASEQAPDAEIVIERACGPYLAGRAAADGVRRLGFESHVV
                     TVEGFDALTKQARDTELVRAPGMVEALREIKDAGEIALLRLACEAADAALADLVGQGG
                     LRAGRTEREVGRDLESLMLDHGADGVSFETIVAAGANSAIPHHRPTDAVLAAGDFVKI
                     DFGALVCGYHSDMTRTFVLEPAAQWQLDLYQLVADAQRAGREAVRPGVALRDVDRASR
                     QVIADAGYAENFTHGLGHGVGLEIHEAPAINASAAGTLLAGSTVTVEPGVYLPDRGGV
                     RIEDTLVVCEAAGEPLGLRHQTSDVLTQFPRELAILD"
     gene            1528..2088
                     /gene="efp"
                     /locus_tag="VGP27_17250"
     CDS             1528..2088
                     /gene="efp"
                     /locus_tag="VGP27_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV7853080.1"
                     /translation="MASTADFKNGLVLNIDGQLWSIVEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDKTFNAGVKVETATVDRRDTTYLYRDGSEFVFMDSRDYEQHHLPESLVGDDA
                     RFLLESLPVQVAFHNGTALYIELPVTVELEVTHTEPGLQGDRSSAGTKPATVETGAEI
                     QVPLFINTGDKLKVDSRDGSYLGRVN"
     gene            2085..>2488
                     /gene="nusB"
                     /locus_tag="VGP27_17255"
     CDS             2085..>2488
                     /gene="nusB"
                     /locus_tag="VGP27_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV7853081.1"
                     /translation="MTRPKRDRGRHQARKRAVDLLFEAEARSITPAEAAQARESLAQN
                     QSDVAALNPYTVTVAQGVTQHSAHIDDLISAHLQGWTLDRLPAVDRAILRVAVWELLH
                     AEDVPEPVAVDEAVELAKHLSTDESPGFVNGVL"
BASE COUNT          449 a          787 c          855 g          397 t
ORIGIN      
        1 gcagtgcggt gaacgctgcg aaggtggtga gctcgttgat cagcccaccc acatagaggc
       61 ccttgtagaa cagcgagatc agccacgcga ccgcgccgct gacgagtccc gcgaacaggc
      121 cggccagcag ccacgtcatc gacaggtctt cgcgacggtc gggatcgggg ttggtccgtg
      181 cgtccgagcg gccgtcgagg aaaccccaga cgaacacgat gatcgtgaaa atgacgacga
      241 gggtgatgct gatcagtccc gccttggtct cccaggcgtt gatcgccgcg ccctgcacca
      301 gtcggatgaa aaccatcagg gccgcgaaca ccagtccgcg caccaaccac ttactcatgg
      361 ggcatcacct tagcgagtag cgtcaagacc tgtgactata tcgcagcgcc gagaccggtt
      421 aaggaagcgg cttgagtccg acggactggc cgcgatgctg gtaacgcgcc tggtcaacgt
      481 gcgttacctg tccggtttca ccggttccaa cgcggcgttg cttattcgcg cggacgacga
      541 cactccggtg ttggccaccg acgggcgcta tcgcacccag gcgagcgagc aggctccgga
      601 cgccgaaatc gtgatcgagc gtgcatgcgg gccgtatctg gccggacggg ctgcggcgga
      661 cggggtgcgt cggttgggct tcgagagtca cgtcgtcacg gtggaagggt tcgacgcgct
      721 gacgaagcaa gcccgcgaca cggagttggt ccgtgcaccg ggcatggtcg aggcgctgcg
      781 ggaaatcaag gacgcaggcg agatcgcgct gttgcggctg gcctgcgagg cggccgatgc
      841 cgcgcttgcc gatctcgtcg gacagggagg gctgcgcgcc ggccgcaccg agcgcgaggt
      901 gggccgcgac ctggaatcgt tgatgctgga tcacggcgcg gacggggtgt cattcgagac
      961 gatcgttgcg gccggtgcga actcggcgat cccgcaccac aggccgaccg atgcggtcct
     1021 cgcggcgggt gatttcgtca agatcgactt cggcgcgctg gtgtgcggat accactcgga
     1081 tatgacgcgc accttcgtgc tcgagcccgc cgcgcaatgg cagctcgacc tctatcagct
     1141 ggtcgccgac gcccagcggg ccggacggga ggcggtgcgg ccgggtgtgg cgctgcgcga
     1201 cgtggaccgc gcgtcgcggc aggtgatcgc cgacgcggga tatgccgaga acttcaccca
     1261 tggtctcggg cacggcgtcg gcctcgaaat ccacgaagca ccggcaatca acgcctcggc
     1321 cgccggtaca ctgcttgctg gctccacggt gaccgtggaa cccggtgtct acttgcccga
     1381 tcgcggcggt gtccgcatcg aggacaccct ggtcgtatgc gaggcggccg gggagccgct
     1441 tggcctgcga catcagacat cagacgtgct tacccagttc cccagggaac tggccattct
     1501 cgattgagag acgaggagta gtaccgcgtg gcatcgaccg ccgacttcaa gaacggtctc
     1561 gttctgaaca ttgatggcca gctgtggtcc atcgtcgagt tccaacacgt caaaccgggc
     1621 aagggccccg cgttcgtgcg caccaagctg aagaacgtgc tgtcgggcaa ggtcgtcgac
     1681 aagacgttca acgcgggggt caaggtcgaa acggcgacgg tggaccgccg cgacaccaca
     1741 tacctctacc gcgacggctc ggaattcgtg ttcatggaca gccgggacta cgagcaacac
     1801 catctgccgg agtcgttggt cggtgacgac gcgcgcttcc tgctcgagag cctgccggtg
     1861 caggtggcgt tccacaacgg gacggcgctc tacatcgagt tgccggtgac cgtcgaactc
     1921 gaggtcaccc acaccgagcc cggcctgcag ggcgaccggt ccagtgcggg cacgaagccg
     1981 gccactgtgg agaccggggc cgagattcag gtgccgcttt tcatcaacac cggtgacaag
     2041 ctcaaggtcg actcgcgcga cggcagttac ctgggccggg tgaattgacc agacccaagc
     2101 gcgacagggg taggcaccag gcccgtaagc gggcggtgga cctgctcttc gaggcggagg
     2161 cccgcagcat cacgcccgca gaggccgcgc aagcgcggga gtcgttggcg cagaaccaat
     2221 ccgacgtggc ggcgctgaac ccgtacacgg tgacggtggc tcagggtgtc acgcagcatt
     2281 ccgcgcacat cgacgatctc atctcggcgc acctgcaggg ttggacgctt gaccgcctgc
     2341 ccgccgtcga tcgggcgatc ctgcgggtcg cggtctggga actgctgcac gccgaggatg
     2401 tgcccgaacc ggtggccgtc gacgaggccg tcgaactggc caagcatctg tccaccgacg
     2461 aatcgcccgg gttcgtcaac ggcgtgct
//
LOCUS       DASYLX010000738         5172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1356017,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000738 DASYLX010000000
VERSION     DASYLX010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356017"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGP27_17260"
     CDS             complement(<1..484)
                     /locus_tag="VGP27_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEV7853082.1"
                     /translation="MGTSTRVPETITISGDELSADDAVTALRRYGRWNLMKNSFIRFR
                     YADGFSHSRALALQLCLSFIPLIIAVVGLAGAVHQQKAGQVIIQTVAGVLPGKDQSDM
                     VQQAAQRTHDQGLTGGHLALWLGLLAAIVALTTAMGQIERGANRIYGIERDRPALRKY
                     G"
     gene            complement(503..1390)
                     /locus_tag="VGP27_17265"
     CDS             complement(503..1390)
                     /locus_tag="VGP27_17265"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV7853083.1"
                     /translation="MRAAIIINPAKSEVGAREQIDGALTAAGWSPSLWLETTPEDPGR
                     GMAEAAVAATVQLIVICGGDGTVMACLSGLADSGVPVAIIPVGSGNLLARNLGIPLGL
                     DESLATAVDGMDRRIDLGRLGDRPFAVMAGMGFDAAMMADTTEGMKRSAGWPAYVVSG
                     LRHLKDPVMQVRICIDDGPMLRRSARTVLVGNVGQLEGGLELLPDAAADDGLLDVAVV
                     APRTLRDWLRVAYRIARRHRHDRHLERFTGQTISIEADRVVPRQIDGEVIDEGRRIDV
                     HVEPGALVVRVPQSAAAAA"
     gene            complement(1390..1956)
                     /locus_tag="VGP27_17270"
     CDS             complement(1390..1956)
                     /locus_tag="VGP27_17270"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7853084.1"
                     /translation="MPGRLVEYRQQGGVSESRYITMLSATPTIIVATVLAAVVSRWMF
                     GRWRESLVVIYAVVGETAVFMATTLFIERPRPAVPHMDQAPPTSSFPSGHTAAAICFY
                     GSVAAIAVWHSRRRWLAVAAVILCAAVPLTIAASRVYRGMHYPTDVFAGAMLGVIWLS
                     IVVFYVRSHDAGGRRHAAVDADRRLVSG"
     gene            complement(2171..2920)
                     /locus_tag="VGP27_17275"
     CDS             complement(2171..2920)
                     /locus_tag="VGP27_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7853085.1"
                     /translation="MPNRLLRALHPHVSAADERFGVRLGAGLAGTLVAAAGFALLLVL
                     VRAGWKPLRSFDVGTAQAFRRIDESHPELVRTAETISRVFDPNVFRIALTVIALIYLI
                     RGERRHATWLVVTVFGGAGLGLGLKLIVGRARPVLSDPVSAAPGPSFPSGHALGASIA
                     CCLFLLLGLRLLSRRGRIAAVAAAVLVVCAVALARVVLGVHFASDVMAGVMLGICWVA
                     VTTWAYVAWRRETGQPVERPADVGSAEQPVR"
     gene            3055..4716
                     /locus_tag="VGP27_17280"
     CDS             3055..4716
                     /locus_tag="VGP27_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853086.1"
                     /translation="MTQELSNEGVAEVVEADLDTMTATKETLEDYTLRFAPRSYRKWS
                     TRVVGISALGGIAYLADFAIGANVGISYGTTNALWGILIFAVVIFLTGFPVAYYSARY
                     NIDLDLVTRGSGFGYYGSVVTNVIFATFTFIFFALEGSIMAQGLQLGLGIPLWLGYAA
                     STLIIFPLVIYGMKVLSTLQVWTTPLWLILMVAPFVYLVISHPESVSQFFAYQGENGK
                     GGFDLGSTLLAAGVCLSLIAQIAEQNDYLRFMPPQTPENKRSWWTWLILAGPGWVFFG
                     AIKQVVGLFLAVYLIANVADSAGIANQPVHQFLEIYENFLPGWLAMTLAVVLVVISQI
                     KINVTNAYSGSLAWTNSFTRVTKHYPGRIVFLGVNLLIALILMEANMFSFLNTILGFY
                     SNCGMAWVVVVASDIVFNKYLLKLSPEKPEFRRGMLYAFNPVGFGSMLIAAGLSVIAF
                     FGGLGEAIRPYSPLVAIGLGLVLPPIIAIATRGKYYLRRTDDGIDLPMYDDLGNPSGE
                     TLKCHVCHHDYERPDMMKSAVHEGHICSLCLSTDKSGEHVLPTQT"
     gene            complement(4713..>5172)
                     /locus_tag="VGP27_17285"
     CDS             complement(4713..>5172)
                     /locus_tag="VGP27_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7853087.1"
                     /translation="PETSSEGITSWDDVKVLDVRLDRLRRWHDKGVLCIGDAAHAMSP
                     AGGVGINLAIADAVATAGILAEPLRTHRLTTKKLAEVRRRRVLPTVATQTVQRIVHNR
                     LLLPALQGREVRPPAALTQLVERVPALTAIPAYVVGVGLLPQHAPDFARR"
BASE COUNT          944 a         1789 c         1590 g          849 t
ORIGIN      
        1 ggccgtactt ccgcagcgcg ggccggtcgc gttcgatgcc gtagatgcgg ttcgcgcccc
       61 gctcgatctg ccccatcgcc gtggtcagcg cgacaatggc ggcgagcaga cccagccaca
      121 acgcgagatg gccgcccgtc aagccctggt catgcgtgcg ctgcgcggcc tgctgcacca
      181 tgtcgctctg atctttgccg ggcaataccc ctgccacggt ctggatgatc acctgccccg
      241 ccttctgctg atgaaccgcg ccggccagcc cgacgacggc gatgatcaac gggatgaacg
      301 acagacacaa ctgcagcgcc agtgcccggg agtgggagaa gccgtcggca taccggaacc
      361 ggatgaacga gttcttcatc aggttccatc gcccgtatcg ccgcagcgcg gtcaccgcgt
      421 cgtcggccga gagttcgtca ccggagatgg tgatggtctc cggcacgcgc gtcgaagtgc
      481 ccattcggat cctcctaacc ggtcacgcgg ctgccgcggc cgactgcggc acgcgcacca
      541 cgagcgcgcc gggctcgaca tgtacgtcga tgcggcgacc ttcgtcgatg acctcgccgt
      601 cgatctgacg gggcaccacc cggtcggcct cgatcgagat cgtttgtccg gtaaagcgtt
      661 ccaggtgccg gtcgtgcctg tggcggcgcg cgatccggta ggccacccga agccagtcgc
      721 gaagcgtgcg aggcgcgacg acagccacgt cgagcagacc gtcgtcggcc gcggcgtcgg
      781 ggaggagctc gaggccgccc tcgagctgcc cgacgtttcc caccaacacc gtgcgtgcag
      841 accgtcgcaa catcgggccg tcgtcgatgc atatccgcac ctgcatcacc ggatctttca
      901 ggtgccgaag gccactgacc acataggccg gccatcccgc ggaccgcttc atgccttcgg
      961 tggtgtcggc catcatcgcc gcgtcgaaac ccatcccggc catcaccgcg aacggccggt
     1021 cgccgaggcg acccagatcg atgcgacggt ccatgccgtc gaccgcggtc gccagagatt
     1081 cgtcaagccc gagcggaatt ccgagattgc gggcgagcag gttgccgctg ccgacaggaa
     1141 tgatcgcgac gggcacaccg ctgtcggcca gcccactcag gcatgccatc acggtgccgt
     1201 cgccgccgca gatcacgatg agttggacag tcgccgcgac agcggcctcc gccattccgc
     1261 gtccggggtc ctcaggcgtc gtctccagcc acagcgacgg cgaccagcct gcggcggtca
     1321 gggcgccgtc gatctgctcc cgcgccccca cctccgactt agccggattg atgatgatcg
     1381 ccgcgcgcat cagccactca ccagcctcct gtcggcatcg acggccgcgt ggcggcggcc
     1441 gccggcgtca tggctccgca catagaagac gacgatgctc aaccagatca cccccagcat
     1501 ggcaccggcg aacacatcgg tcgggtaatg catgccccgg tacacacgac tggccgcgat
     1561 ggtcagcggt actgccgcac agaggatcac ggccgccacc gccagccaac gccgccggct
     1621 gtgccagacg gcgatcgctg cgaccgagcc gtagaagcag atcgccgcgg ccgtatgccc
     1681 cgaggggaac gacgacgtcg gcggcgcctg gtccatatgc ggcacagccg gtcgcggccg
     1741 ctcgatgaac agcgtcgtcg ccatgaagac ggctgtctca cccaccaccg catagatgac
     1801 gacaagggat tcacgccatc ggccgaacat ccagcgcgac accaccgccg caagcaccgt
     1861 cgccacgatg atcgtcggcg tagcggacag catggtgatg tacctgcttt ccgacacgcc
     1921 accctgctgg cggtactcga ccagccgccc cggcatgctc gcatcccatc tgccgaaggc
     1981 actagacggc attacgtgcg tgatgagata acccacggtc accatgaacg cgacaagaca
     2041 aacggccggg acgacaagcc acccgacatc tcgtgctacg cccccgacac tcttcgagcg
     2101 cacggatgag aagtacccgg tcgcgcgccg atttactcaa ctgtcaaatc ttgacgggtg
     2161 aagtcccagc tcagcgcacc ggctgctcgg cgctacccac gtcagcgggc cgctctaccg
     2221 gctgcccggt ctcgcgtcgc cacgccacat aggcccacgt cgtcacggcg acccagcaga
     2281 tgccgagcat caccccggcc atcacatcgc tggcgaaatg gacgccgagc accacacgtg
     2341 ccagcgcgac ggcgcagacg accaggactg ctgcggcaac ggcggcgatg cggccgcggc
     2401 gggacagcaa ccgcagcccc agcaacagga agagacagca cgcgatcgac gcgccgagcg
     2461 cgtgaccaga gggaaacgat ggacctggcg ctgccgaaac cggatcagaa agaacgggac
     2521 gggcccggcc cacgatcaat ttcaacccga ggccgaggcc cgcgccgccg aacacggtga
     2581 cgaccagcca tgtcgcgtgg cgacgttcgc cgcggatgag atagatcagc gcgatgaccg
     2641 tcagcgcgat ccgaaacacg ttcgggtcga agacacgact gatggtctcg gcggtgcgga
     2701 cgagttcggg atgagattcg tcgatccggc gaaatgcctg tgccgtgccg acgtcaaagc
     2761 tccgcaatgg cttccagccg gcacgaacca gcacgagcag tagcgcgaag cccgctgcgg
     2821 cgacgagcgt tccggccagt cctgcgccga gtcgcacccc gaaccgctcg tcggcggcgg
     2881 acacgtgcgg gtgcagagcc ctcaagagcc tgttcggcat gactgagtag ttcccccaac
     2941 ggcgacttgg tatgcccccg ggggtgcctg agctgattcc tctgacaagg gccatttcac
     3001 actgcggaac atcggcgtca cggttcggaa acaaacccgt tctacgttcg ccgaatgacc
     3061 caggagctca gcaacgaagg cgtcgccgag gtcgtcgagg ccgacctcga cacgatgacg
     3121 gcgaccaaag agacgctcga ggactacaca ctgcgcttcg ctccacggag ctaccgcaag
     3181 tggtcgacgc gcgtcgtcgg catctcggcg cttggcggaa tcgcctacct ggcggacttc
     3241 gccatcggcg ccaacgtcgg aatctcttac ggcacaacca atgcgctctg gggcatcctg
     3301 atcttcgccg tagtgatctt cctgaccggc ttcccggtgg cgtactactc ggcgcgctat
     3361 aacatcgacc tggacctggt gacccgcggc agcggcttcg gctactacgg ctccgtcgtc
     3421 accaacgtca tcttcgcgac gttcacgttc atcttcttcg cgctcgaggg ctcgatcatg
     3481 gcccagggcc tccaactcgg cctcgggata ccgctgtggc tgggttacgc ggcgtccacg
     3541 ctgatcatct tccctctggt catctacggc atgaaagtcc tttcgacact ccaggtatgg
     3601 acgactccgc tgtggctgat cctcatggtg gcgccgttcg tctacctggt catcagccat
     3661 cccgagtcag tcagccagtt cttcgcctac cagggcgaga acggcaaggg cggtttcgat
     3721 ctcgggtcga ctctgttggc cgccggtgtg tgtctgtcac tgatcgcaca gatcgccgag
     3781 cagaacgact atctgcgctt catgccgccg cagaccccgg agaacaagcg cagttggtgg
     3841 acatggctga tcctcgccgg ccccggctgg gtgttcttcg gcgccatcaa gcaggtcgtc
     3901 ggtctgttcc tggccgtcta cctgatcgcg aatgtggccg acagcgcggg catcgccaac
     3961 cagcctgtgc accaattcct cgagatctat gagaacttcc tgcccggctg gctcgccatg
     4021 acgctggcgg tggtgctcgt ggtcatcagc cagatcaaga tcaatgtgac caatgcgtac
     4081 tccggatcgc tggcgtggac caactcgttc acccgcgtca ccaagcacta ccccggacgc
     4141 atcgtcttcc tgggggtcaa tctgctgatc gcgctgatcc tgatggaagc caacatgttc
     4201 agcttcctga acaccattct cgggttctac tcgaactgcg gtatggcatg ggtcgtcgtg
     4261 gtggccagcg acatcgtatt caacaaatac ctgctgaagc tctcgcccga gaagcccgag
     4321 ttccgtcggg gcatgctcta cgcgttcaac cccgtcgggt tcggctcgat gctcatcgcc
     4381 gcgggtctgt cggtcatcgc gttcttcggc ggcctcggcg aagccatccg gccgtactcg
     4441 ccgctggtgg ccattggtct cggcctggtt ctgccgccga tcatcgcgat cgccacgcgc
     4501 ggcaagtact acctgcgccg gaccgacgac ggcatcgacc taccgatgta cgacgacctc
     4561 ggcaacccct ccggcgagac gctgaaatgc catgtctgcc accatgatta cgagcgtccg
     4621 gacatgatga agtcagccgt gcacgaggga cacatctgct cgctgtgcct ctccaccgac
     4681 aaatccgggg agcacgtgct gcctacgcag acctagcgcc gggcgaaatc cggcgcgtgc
     4741 tggggcagca aaccgacgcc gacgacgtag gcaggaatgg ccgtcagcgc agggacacgc
     4801 tcgaccagtt gcgtcaacgc cgcgggcggt cgcacctcgc gaccctgcag cgccggtagc
     4861 agcagacggt tgtgcacgat gcgctgcacg gtctgggtgg ccacggtggg tagcacccga
     4921 cgccgacgta cctcggccag cttcttggtc gtaaggcgat gtgtccgaag cggttcggcc
     4981 agaatgcccg cggtggccac tgcgtcggcg atcgccaggt tgattccgac gccgcctgca
     5041 ggcgacatgg catgtgcggc gtctccgata cacagcacgc ccttgtcatg ccaacggcgc
     5101 aggcgatcga ggcgcacgtc gagaactttc acgtcgtccc acgacgtgat gccctccgac
     5161 gaagtttcgg gg
//
LOCUS       DASYLX010000739         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2672626,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000739 DASYLX010000000
VERSION     DASYLX010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672626"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGP27_17290"
     CDS             <1..341
                     /locus_tag="VGP27_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853088.1"
                     /translation="MTWLLAGLFAGLVSGAVAWLISLFYKGLYVGGLINELTTFAAFT
                     ALLTFLAAIAGVAIGHWVVDRHAEDEPRQRHGSGAGDDERADTDVFAAVRHPDAPADE
                     TPTERRQEQT"
     gene            complement(385..816)
                     /gene="aroQ"
                     /locus_tag="VGP27_17295"
     CDS             complement(385..816)
                     /gene="aroQ"
                     /locus_tag="VGP27_17295"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEV7853089.1"
                     /translation="MTTVNVINGPNLGRLGRRETQVYGSTTHDDLVAMIEREAAELGL
                     KAVVRQSDSEAELLNWIHLAADAGEPVVLNAGALTHTSIALRDACAELRAPLIEVHIS
                     NVHAREEFRHHSYLSAIATGVIVGLGAQGYLLALRYLASRD"
     gene            complement(813..1901)
                     /gene="aroB"
                     /locus_tag="VGP27_17300"
     CDS             complement(813..1901)
                     /gene="aroB"
                     /locus_tag="VGP27_17300"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV7853090.1"
                     /translation="MTDLTAPVTVEVKTDPPYPVVIGTGLMGELGSVLEGRHKVAILH
                     QPTLAQTAEAIRNHLSDKGVDAHRVEIPDAEAGKELPVLGFIWEVLGRIGIGRRDAVV
                     SLGGGAASDVAGFAAATWLRGIDVVHVPTTLLGMVDAAVGGKTGINTDAGKNLVGAFH
                     QPAAVLVDLATLETLPRNEIVAGMAEIVKAGFIADPVILDLIEADPEAALDPTGQVLP
                     ELIRRAVTVKAEVVAADEKESQLREILNYGHTLAHAIERRERYQWRHGAAVSVGLVFA
                     AELGRLAGRLDDDTAERHRAVLTSLGLPVGYDADALPQLIEYMAGDKKTRSGILRFVV
                     LDGLAKPGRLEGPDPSLLAAAYAEVAQA"
     gene            complement(2056..2556)
                     /locus_tag="VGP27_17305"
                     /pseudo
     CDS             complement(2056..2556)
                     /locus_tag="VGP27_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            complement(2556..>2814)
                     /locus_tag="VGP27_17310"
     CDS             complement(2556..>2814)
                     /locus_tag="VGP27_17310"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV7853091.1"
                     /translation="STVPRALSTVDMATGDEAVAIHQRSDVCAVPAAGVVVETMVALV
                     LARAALEKFGGDSLAETRANLETYLRSLSAREPAQRSQASG"
BASE COUNT          436 a          971 c          942 g          465 t
ORIGIN      
        1 cgatgacgtg gctgctggcc ggcctgttcg cgggactcgt cagcggcgcg gtcgcgtggc
       61 tgatctcgct gttctacaag ggcctctatg tgggtgggct gatcaacgag ctcaccacct
      121 tcgcagcgtt caccgcactg ctgaccttcc tcgcggcgat cgccggggtc gcgatcggcc
      181 attgggtcgt cgaccggcac gccgaagacg aaccgcgtca gcggcacggc tccggcgccg
      241 gcgatgacga gcgcgcggac accgacgtgt tcgccgccgt gcggcatccg gacgcgcccg
      301 ccgacgagac gccgaccgag cgtcgccagg aacagaccta agccccacgt cacgccgcgg
      361 ccgcgcccga aaggcgctgg agcgctagtc gcgcgaggcg aggtagcgca gcgcgagcag
      421 gtagccttgc gcgccgagtc cgacgatgac gccggtggcg atcgcgctga ggtacgagtg
      481 gtgcctgaac tcttctctcg catgcacatt ggagatgtgc acttcgatca gcggggcgcg
      541 cagttcggcg cacgcatccc tcaaggcgat cgaggtgtgc gtcagcgcgc ctgcgttcaa
      601 cacgaccggc tcgcctgcgt cggcggcaag gtggatccag ttcaacagtt cggcttcgct
      661 gtccgattgc cgcacaaccg ctttgagccc gagctcggcg gcctcacgct cgatcatcgc
      721 gacgagatcg tcgtgcgtcg tgctgccgta gacctgcgtc tcacgccggc ctaggcggcc
      781 caggttcggg ccgttgatga cattcaccgt cgtcatgcct gtgccacctc cgcgtacgcc
      841 gccgcgagca atgagggatc cggaccttcc agccgtcccg gcttcgccag cccgtcgagg
      901 acgacgaagc gcaatattcc cgagcgagtc ttcttgtcac cggccatgta ttcgatcaac
      961 tgtggcagcg cgtcggcgtc atagccgacc ggaaggccga gcgacgtcaa cacggcgcgg
     1021 tgccgctcgg cggtgtcgtc gtcgagtcga ccggccagcc tgcccagttc agcggcgaac
     1081 accaggccga ccgacacggc ggcgccgtgg cgccactggt agcgctcgcg gcgctcgatg
     1141 gcgtgggcca aagtgtggcc gtagttcagg atctcgcgca actgcgattc cttctcgtcg
     1201 gcggcgacca cttccgcctt gaccgtcacg gcgcggcgga tcagttccgg caggacctga
     1261 cccgtcggat ccagtgcggc ctcgggatcg gcttcgatga gatcgaggat caccgggtcg
     1321 gcgatgaaac cggccttcac gatctccgcc atgcctgcga cgatctcgtt gcgcggcaac
     1381 gtctccaatg tcgccaggtc gacgagcacc gcggcgggct ggtggaatgc cccgacgagg
     1441 ttcttccccg catcggtgtt gatgccggtt ttcccgccga ccgcggcgtc caccatgccg
     1501 agcaacgtcg tcggcacatg cacgacatcg attccacgca gccaggtggc ggcggcgaat
     1561 cccgcgacat cggacgccgc tcccccgccg aggctgacga cggcatccct gcggccgatg
     1621 ccgatgcggc cgagcacttc ccagatgaag ccaaggacgg gcagttcctt gcccgcttcg
     1681 gcgtcgggga tctcgacgcg atgcgcgtcg actcccttgt cggacagatg gttacggatt
     1741 gcctctgccg tctgcgccag cgtcggctgg tgcagaatcg cgaccttgtg cctgccctcg
     1801 aggacgctgc ccaactcccc catcaagccg gtgccgatga ccaccggata cggcgggtcg
     1861 gttttcacct cgacggtgac gggcgccgtc aggtcagtca tggcgggcct cggcgcggcg
     1921 cgccaactcg gccggactgg cgggcgtggt ggcctccgat gcgctcggcg tcagcgatac
     1981 cggggtgcga cgccacgacg gacgccggcg ccgtcgcggc ttggttttgg gcggcgcatc
     2041 gtccgcttgc ggattctcca gtcgcgacgc gatgtagcgc accactgcgc cgggattgcg
     2101 tcggttggtg ttgacccgga tcgtcgccga tttgcggtac agcggaacac gctgggacat
     2161 caatgcgcgg aacttctctg cgcgatcgcc accggccagc aacggccgca cggtggtacc
     2221 gccggtgcgg cgaacccctt cggccgcgct gatctcgagg tagaccacag tgtggccgga
     2281 aagcgcctcg cgcacaccgg gactggtaat ggcaccgccg cccagcgaaa gcacgccttc
     2341 gtgtgatccc aaggcctcgc ggacgacttc ttcctcgatg cgccggaact cctgttcgcc
     2401 gtcgttggcg aagatgtcag gaatggcgcg gccggtcgtc tgctcgatcg ccgcatcggt
     2461 gtccaataat tcgacgccga gcgacttggc caaccggcga ccgatcgtgg acttgcccga
     2521 accgggcagc ccgatcagta ccgccttggg tgccatcagc ccgacgcctg agagcgttga
     2581 gccggttcgc gcgcagagag cgaccgcaga tacgtttcga ggttggctcg ggtctcggcc
     2641 aacgagtcgc caccgaactt ctccagcgcc gcgcgggcaa gcaccagcgc caccatggtc
     2701 tcgaccacga cgccggcggc aggcacggca cacacgtcgg agcgctggtg aatcgcgacg
     2761 gcctcgtcgc ccgtggccat gtcgacggtg gacagggccc gcggcaccgt cgag
//
LOCUS       DASYLX010000740         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2563017,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000740 DASYLX010000000
VERSION     DASYLX010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563017"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGP27_17315"
     CDS             <1..432
                     /locus_tag="VGP27_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEV7853092.1"
                     /translation="QGDVFLAERAYDVHLLNINNSGLSISGKNVLAFSSSLDWNIERV
                     KGGSIATGGLFNTTLRGTGWVALTTDGPPVVLNAAEAPTFADANAVVAWSAHLQTQLK
                     TSFKAGALIGRGSGEAVQVSFHGQGFVIVQPSEGAQVTATA"
     gene            complement(433..864)
                     /gene="dtd"
                     /locus_tag="VGP27_17320"
     CDS             complement(433..864)
                     /gene="dtd"
                     /locus_tag="VGP27_17320"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017206132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV7853093.1"
                     /translation="MRILVQRVTSASVTVDGETVGAIEPDGQGLLALVGVTHDDNTSK
                     AQRLAEKLWQLRILDDENSAADVNAPVLVVSQFTLYANTAKGRRPTWNAAASGAVAEP
                     LVTAFADALRGLGAHVETGVFGADMKVALVNDGPVTVQLEL"
     gene            complement(875..1519)
                     /locus_tag="VGP27_17325"
     CDS             complement(875..1519)
                     /locus_tag="VGP27_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV7853094.1"
                     /translation="MWKRAVLTGAGAVAAMVIAAGCSDGGAGTGHSAGQGSSASSSVT
                     AAPSSQDRNDADVMFVHHMIPHHQQAIEMSDVILGKRGIDPRVTELATEIKGAQGPEI
                     QQMQDWLGQWGNPPMPSMAASGGNGGHEMDGMSGDGMGMMSDEQMTALRSAEGVEASR
                     SFLIGMIKHHEGAIDMAQNEIGNGRFQPAVQLARAIVKSQQQEIDTMKGILETL"
     gene            1583..>2328
                     /locus_tag="VGP27_17330"
     CDS             1583..>2328
                     /locus_tag="VGP27_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV7853095.1"
                     /translation="MPPTADTTDRHKLTLAVSGMSCASCATRIEKRLNQLDGVVATVN
                     FATEQATVDFPDRITPDNLVDAVQETGYTATLPARSEDQTAETTAWKRRLLISAALTV
                     PVVLLSMVPALQFDYWQWVALALATPVAAWGAWPFHRAAVANLRHRAATMDTLISVGV
                     AAAYLWSVWALLFTHAGTPGMKMTLSLIANDDGGAHLYLEVAAAVTTFILAGRYFESR
                     AKRSSGAALRALLDAGAKDVAVLREGSTQTR"
BASE COUNT          368 a          786 c          749 g          425 t
ORIGIN      
        1 cagggcgacg tcttccttgc cgagcgcgcc tatgacgtgc acctgctgaa catcaacaac
       61 tccgggctgt cgatcagcgg gaagaacgtg ctggcgtttt cgtcgagcct ggactggaac
      121 atcgagcggg tgaagggcgg cagcatcgcg acaggcgggc tgttcaacac gacgctgcgc
      181 ggcaccggtt gggtggcgtt gacgacggac ggtccgcctg tggtgctgaa cgccgccgag
      241 gcaccgacgt tcgccgacgc caacgcggtc gtggcctggt cggcgcacct gcagacgcaa
      301 ctgaagacga gcttcaaggc gggtgcgttg atcggtcgcg gatccggtga agcagtccag
      361 gtgtcgttcc acgggcaggg tttcgtgatc gtgcagccct cggagggtgc tcaggtcacc
      421 gcgacggcgt agtcacagct ccagctgcac tgtgacgggg ccgtcgttga cgagtgcgac
      481 cttcatgtct gcgccgaata cgccggtctc gacgtgcgcg ccgagccctc gtagcgcgtc
      541 ggcgaaagcg gtcaccagtg gctcggcgac ggcgccggac gcggcggcgt tccacgtcgg
      601 gcgcctgccc ttggcggtgt tggcgtagag ggtgaactgg ctgacgacca ggacgggtgc
      661 gttgacgtcg gccgccgaat tctcgtcgtc gagaatgcgc aattgccaga gcttttcggc
      721 gagccgctgg gccttgctcg tgttgtcgtc gtgcgtgaca ccgaccagcg cgagcaagcc
      781 ttggccgtcg ggttcgatcg cgccgacggt ttcgccgtcg accgtcaccg aggccgaggt
      841 cacccgctga acgaggattc gcacggcgac gacgctacag cgtctcgagg atccccttca
      901 tcgtgtcgat ctcctgctgc tgggacttga cgatggcacg cgcgagctgg accgccggct
      961 gaaaccgacc gttcccgatc tcgttctgcg ccatatcgat tgcaccctca tggtgcttga
     1021 tcatgccgat caggaatgac ctgctcgcct cgacgccctc ggcgcttcgc agcgccgtca
     1081 tctgttcgtc ggacatcatg cccatcccgt cgccggacat gccgtccatc tcgtggccac
     1141 cgttgccccc gcttgcagcc atggacggca tcggcggatt gccccactgg ccgagccagt
     1201 cctgcatctg ctggatctcg ggaccctgtg cacccttgat ttcagtggca agttcggtga
     1261 cccggggatc gatgccccgc ttgcccagga tgacatcgct catctcgatc gcctgctgat
     1321 gatgcggaat catgtggtgg acgaacatga cgtccgcgtc gttgcgatcc tggctgctgg
     1381 gcgccgcggt taccgacgac gacgccgacg acccctgtcc cgccgaatgc cctgttcctg
     1441 ccccgccatc actacagccg gccgcgatca ccatcgccgc cacggcgcct gcacccgtca
     1501 gaactgctcg tttccacatc acgtctgcga tcccttcgtc gtcaccttta cctatacccc
     1561 ctacgggtat aggctggcaa ctatgccgcc gaccgccgac accaccgacc ggcacaagct
     1621 caccttggcc gtcagcggga tgtcgtgtgc ctcgtgtgcg acgcgcatcg agaagcggct
     1681 caaccagttg gatggcgtcg tcgcaaccgt caacttcgcg accgagcagg caacagtcga
     1741 cttcccggac agaatcaccc ccgacaacct ggtcgacgcg gtccaagaaa ccggttacac
     1801 cgcaacgcta cccgcgcgta gtgaagacca aaccgccgag acgacggcgt ggaagcgccg
     1861 cctgctgatc tcggccgcgc tgaccgttcc ggtggtgctg ctgtcgatgg tgcccgcgct
     1921 gcagttcgac tactggcagt gggtggcgct ggccctcgcc acgcccgttg cggcgtgggg
     1981 cgcctggccg tttcatcgcg cggcggtggc caacctgcgc caccgggcgg cgacgatgga
     2041 cacgctgatc tcggtcggcg tcgccgcggc ctatctctgg tcggtctggg cgctgctgtt
     2101 cacccacgca ggcaccccgg gcatgaagat gacactgagc ctgatcgcga acgacgacgg
     2161 cggtgcgcat ctctacctgg aggttgccgc agccgtcacc acgttcatcc tcgccggtcg
     2221 ctatttcgag tcacgcgcca agcggagttc tggcgcggcg ctgcgcgcat tgctcgacgc
     2281 gggcgccaaa gacgtggctg tgctgcgcga agggtcgacg caaacacg
//
LOCUS       DASYLX010000741         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1246205,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000741 DASYLX010000000
VERSION     DASYLX010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246205"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGP27_17335"
     CDS             complement(<1..434)
                     /locus_tag="VGP27_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7853096.1"
                     /translation="MSEIRPPRYLKPMNRVVRAMQRLGVRTGPAMILTVPGRKTGRPR
                     STPMTPFTYRGDLYTVAGYPGADWAANARAAGAGTLTRGRKSREIRIVELNADESRPI
                     LRAFPVQVPVGVAFAKRSGLVRQGTPDEFEALAGQLAVFRFDP"
     gene            complement(450..1289)
                     /locus_tag="VGP27_17340"
     CDS             complement(450..1289)
                     /locus_tag="VGP27_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7853097.1"
                     /translation="MNIILVTGATGNVGRPLVNQLAEAGVHVRAVTRRPDSAQFPVDV
                     EVVRSAAAGIDGASAVFLNSRALGDELATVVELARRAGVRRLVALSAINADDDFSRQP
                     SRFRGDRNKEVEQHAVDSGLEWVSLRPTVFATNFIGMWAPQIRAGDVVSGPYASASTA
                     PIVDADISAVALHALLHDDLLGRKVPLTGPQALTNTELVGVIGDVLGRPLRYQEVPPD
                     LVRRRFIDLGFSAEFADGYIALLEATVSKPALVTNEVEKILGRPPQTFADAIRPHRDL
                     FAD"
     gene            1370..2227
                     /gene="sigI"
                     /locus_tag="VGP27_17345"
     CDS             1370..2227
                     /gene="sigI"
                     /locus_tag="VGP27_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigI"
                     /protein_id="HEV7853098.1"
                     /translation="MTESPSVEAAWRSHRPYLVNLAYQMLGDVGEAEDVAQETFLRLS
                     RTDIDDIDDVRGWLTVVAGRLCLDQLRSARARHERPENVASDDMVGPKAPDPADRVTL
                     DDEVREAMLDLLRRLSPGERVTFVLHDVFQVPFEEIATTVGRPVGTCRQLARRARGKF
                     AAASPRLTEVADAEHQLVTEKFITACANGDLDGLTAVLDPAVWGVGTVLGEGAPPPQV
                     NHGPDDVGRNLLRYLGHGATLVSGPVGEPVLLAFAERRLFAVIVLTLRGNRVFKIEAS
                     VDVTAAARP"
     gene            complement(2267..2851)
                     /locus_tag="VGP27_17350"
     CDS             complement(2267..2851)
                     /locus_tag="VGP27_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEV7853099.1"
                     /translation="MTKMKNSIAAAGLAAAAAMGMSLTFAGTASAELVGPSCTAYAEK
                     VPTGPGSVAGMAMDPVAVAASNNPMLTTLTKAVSGQLNPNVNLVDTLNGGEFTVFAPT
                     DDAFAKLDPATIEKLKTDSDLLTSILTYHVVSGQMSPAQIVGTHKTVQGADLTVTGMG
                     NNLKVNGASVVCGGVATANAQVYMIDTVLLPPMN"
     gene            complement(2964..4712)
                     /locus_tag="VGP27_17355"
     CDS             complement(2964..4712)
                     /locus_tag="VGP27_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEV7853100.1"
                     /translation="MFTLVLIGLLGGLITGISPCILPVLPVIFFSGAQSTHSEPVAES
                     EGGIAVAVKSKPALSERLRPYRVIGGLVLSFSLVTLIGTSLLSLLHLPQDAIRWAGLI
                     ALTVIGLGLIFPRFEEILEKPFSHIPQLQMSEGRSGFGLGLALGVVYVPCAGPVLAAI
                     VVAGATGAVGIDTVALTISFAIGAALPLLFFALAGQRVAERVGTFRRHQRRIRVIGGV
                     VMLAFALALVFDVPATLQRAIPDYAGSLQDKVVASDEVQERLDLGGLVNDQNRELSKC
                     SSGGAELERCGTAPDIKGITGWLNTPDGAPVDLKSLRRKVVLVDFWAYSCINCQRAIP
                     HVVDWYGKYKDVGLEVVGVHSPEYAFEKIAGNVAGGAEDLGITYPVALDNNLSTWTNY
                     RNRYWPAEYLIDANGTVRHIKFGEGDYDVTENLIRELLVDANPDVQLPPPSDSADQTP
                     SAGQTPETYFGVGKMVNYGGPDTYSEGTAEFTYPTRLADDSFAYRGRWKLDFQGATAE
                     SDDSRISLRYNAKNVYLVVGGEGDVTVTSNEETVNVPISGAPTSHQLVSGDAVARGEL
                     EVRLSEGLQAFSFTYG"
     gene            complement(4842..5507)
                     /locus_tag="VGP27_17360"
     CDS             complement(4842..5507)
                     /locus_tag="VGP27_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEV7853101.1"
                     /translation="MRNVHRSTLTAATIAALAVLGLSACSSDSSTSERPAAAETTSSA
                     APATTMQAKADPAANLVGTGCAAYAEQVPEGPGSVAGMAQDPVTVAASNNPMLKTLTQ
                     ALSGKLNPNVNLVDTLNGGEFTVFAPTDDAFAKIDPATIEKLKTDSDLLTSILTYHVV
                     PGQASPSKVVGAHNTVQGGEVTVTGEGNGLKVNDAGLVCGGVSTANAQVYMIDTVLMP
                     PTS"
     gene            5714..>5963
                     /locus_tag="VGP27_17365"
     CDS             5714..>5963
                     /locus_tag="VGP27_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV7853102.1"
                     /translation="MSTAHARSTTDVRLRVLILGSTGSIGTQALDVIAANRDRFEVVG
                     LAAGGGSPDLLVRQRMETGVTNIAVADPDAADKVGDVTY"
BASE COUNT         1015 a         1928 c         1962 g         1058 t
ORIGIN      
        1 gggtcgaacc tgaacaccgc caactgtccg gcgagcgcct cgaactcgtc gggcgtcccc
       61 tgacgcacga gcccggagcg tttggcgaag gcgacgccga cgggaacctg caccgggaac
      121 gcacgcagta tcggccgtga ctcgtccgcg ttgagctcga cgatcctgat ctcccgcgac
      181 ttgcgcccac gggtaagcgt gcccgcaccg gcggcccgcg cgttcgccgc ccagtccgca
      241 ccgggatatc ctgcgacggt gtacaagtcg ccgcgatagg tgaacggtgt catcggggtg
      301 ctgcggggcc tgccggtctt cctgcccggc accgtgagga tcattgccgg accggtacga
      361 acgcccagcc gctgcatcgc cctcacgacc cggttcatcg gcttgaggta gcgcggtggc
      421 cgaatttctg acatgtcttc tctcctttgt taatcggcga acagatcgcg gtgaggtcga
      481 attgcgtcgg cgaaagtctg cggaggccgg ccgaggatct tctccacctc gttggtgacc
      541 agcgccggct tggacaccgt cgcctcgagc agcgcgatgt acccgtcggc gaactctgcc
      601 gaaaacccga gatcgatgaa gcggcggcgc acgagatcgg gtggcacctc ctggtagcgc
      661 agcggccgac cgagcacgtc gccgatgacg ccgacgagct cggtgttggt gagggcctgc
      721 ggaccggtca gcggaacctt gcgccccagc aggtcgtcgt gcagcagtgc gtgcaaggcc
      781 accgccgaga tgtcggcgtc gacgatgggg gcagtcgagg ccgacgcata cggtccgctg
      841 acaacatcgc ctgctctgat ctgcggagcc cacatgccga tgaaattggt ggcgaacacg
      901 gtcggccgca ggctgaccca ctccagacct gagtcgacgg cgtgctgctc gacctccttg
      961 ttgcggtcgc cgcggaagcg tgacggctga cgggagaagt cgtcgtctgc gttgatggcc
     1021 gacagcgcga caagccgtct gacgcctgcg cggcgggcca gttcgacaac cgtcgcgagc
     1081 tcatcgccca gcgcgcggga gttgagaaac accgcagatg cgccgtcgat gcccgcggcg
     1141 gccgaccgga ccacctcgac gtcgacggga aactgcgcgg aatcgggtcg acgggtgacg
     1201 gcgcgcacgt gcacgccggc ctccgccagc tggttgacaa gagggcgccc gacgttgccg
     1261 gttgcgcccg tgacgagaat gatgttcatg caaccaagga cggggcgacg agcgcagaag
     1321 ttacgtccgg ccctggtaac ttttcgcgcc gctatttcgt cgtgagtaca tgaccgaatc
     1381 accgtcggtg gaagccgcgt ggcgcagcca tcgcccctac ctggtgaacc tcgcctacca
     1441 gatgctcggg gatgtcggcg aggccgagga cgtcgcgcag gagacattcc tgcggctgtc
     1501 gcgaacagac atcgacgaca tcgacgatgt tcgcggctgg ctcacggtgg tcgcagggcg
     1561 gctgtgcctc gaccagctgc ggtcggcccg cgcccgccac gagcgccccg agaacgtggc
     1621 atcggacgac atggtcgggc caaaggcgcc ggacccagcc gaccgcgtca cgctcgacga
     1681 cgaggtccgc gaagcgatgt tggacctgtt acggcggctg tcgcccggtg agcgcgtgac
     1741 gttcgtgctg cacgacgtct tccaggtgcc gttcgaggag atcgctacga cggtcggtcg
     1801 gcccgtgggc acctgccgcc aactcgcccg acgcgcacgg ggcaagttcg cggcggcctc
     1861 accgcggctg accgaagtcg ccgatgccga acaccaactc gtcaccgaga aattcatcac
     1921 cgcgtgcgcc aacggtgatc tcgatgggct gacggccgtg ctggatcccg ctgtgtgggg
     1981 ggtcggcacc gtactgggcg agggagctcc gccgcctcag gtcaaccatg gcccggacga
     2041 cgtgggccgc aacctgcttc gctacctcgg acatggcgcg acgcttgtca gtgggccggt
     2101 cggcgagccg gttctgctgg ccttcgccga acggcgactg ttcgccgtca tcgtgctcac
     2161 gctgcgcggc aaccgggtgt tcaaaatcga agcaagcgtc gacgtaacgg cagcagcccg
     2221 accgtgatcg gtcgggctgc tccggggtat tctgttgtgg tctagatcaa ttcatcggtg
     2281 gcagcagcac ggtgtcgatc atgtacacct gtgcgttcgc cgtggcgact ccgccgcaca
     2341 ccaccgaggc gccgttgacc ttgaggttgt tgcccatacc ggtcaccgtc agatcggcgc
     2401 cctggacggt cttgtgcgtt ccgactattt gtgccgggct catctggccc gaaaccacgt
     2461 ggtaggtgag gatgctggtg agcaggtccg agtcggtctt gagcttctcg atcgtcgccg
     2521 ggtcgagctt ggcgaacgca tcgtcggtcg gcgcgaagac ggtgaactcg ccgccgttga
     2581 gggtatcgac caggttgacg ttcgggttga gttggcccga cacagccttg gtcagcgtgg
     2641 tcagcatcgg gttgttcgat gccgccaccg cgaccggatc catcgccatc ccggccactg
     2701 aaccggggcc tgtcggtacc ttctcggcgt aggcggtaca tgacggccca accagttcgg
     2761 cggacgcggt gcccgcgaag gtcagcgaca tgcccatcgc cgctgccgcg gccagaccag
     2821 cagccgcaat cgagttcttc atcttcgtca tatgttttcc acttcctgta atggaaggca
     2881 gcgctcgccg cctcacctcg ttgtgcttct catcagtcat tcggagcagg tgcgtgcgcg
     2941 gatgggtgat gtgggtggaa acatcagccg taggtgaatg aaaatgcttg cagtccttcg
     3001 ctcaaccgca cttcaagctc tccgcgcgcg actgcgtcac cggacaccaa ctggtgcgac
     3061 gtcggcgcgc cgctgatcgg cacgttcacc gtctcttcgt tgctcgtcac ggtcacatcg
     3121 ccctcgccac cgacgacgag gtagacgttc ttcgcgttgt atctcaatga aatccgcgag
     3181 tcgtccgact ccgccgtcgc gccctggaag tccaacttcc agcgtccgcg ataggcgaag
     3241 ctgtcgtcgg ccagtctcgt cgggtaggtg aactccgcgg tgccctcgct gtacgtgtcg
     3301 ggaccgccgt agttcaccat cttgccgacc ccgaaatacg tctcgggtgt ctgaccggca
     3361 gaaggcgtct ggtcggcgga atcactcggc ggcggcaact gcacatcggg gttcgcgtcg
     3421 accagcagtt ccctgatcag gttctcggtg acgtcgtagt cgccctcgcc gaactttata
     3481 tggcgcacag tgccattggc gtcgatgagg tattcggcgg gccagtagcg gttgcgatag
     3541 ttggtccagg tcgacaggtt gttgtccagc gcgaccgggt aggtgatgcc cagatcctcg
     3601 gcgccgccgg cgacattgcc cgcgatcttc tcgaatgcgt actcggggct gtgcacaccg
     3661 acgacctcga ggccgacgtc tttgtacttg ccgtaccagt cgaccacgtg cgggatcgca
     3721 cgctggcagt tgatgcacga gtacgcccag aaatcaacca gaaccacctt gcggcgcagc
     3781 gatttcaggt cgaccggagc accgtccggc gtgttcagcc aaccggtgat tcccttgata
     3841 tccggcgccg tgccgcaacg ttcgagctcc gcccccccgc tgctgcattt cgacaattcc
     3901 cggttctggt cgttgaccag gccgccgagg tcgagcctct cctgaacctc atcggacgcc
     3961 acgaccttgt cctgcaatga gcccgcgtaa tcggggattg ctcgttgcag cgtggcgggc
     4021 acgtcgaaca ccagcgcgag cgcaaacgcc agcatcacga cgccaccgat cacgcggatg
     4081 cgacgttggt ggcgtcggaa cgtgccaacc cgttcggcga cccgctgacc ggccagagcg
     4141 aagaacagca gcggcagcgc ggcgccgatg gcgaacgaga tggtcaacgc gacggtgtcg
     4201 atgccgacgg ctcccgttgc accggccacc acgatcgcgg cgagaaccgg tcccgcgcag
     4261 ggtacgtaca ccacgcccag cgccagtccc agtccgaaac cgcttctgcc ctcgctcatt
     4321 tgaagctggg gaatgtggct gaacggtttc tccagaatct cctcgaagcg ggggaagatc
     4381 aaaccgaggc cgatgacggt cagtgcgatg agcccggccc acctgatggc gtcctgcgga
     4441 agatgcaaaa gtgacagcag cgaggtcccg atgagcgtga ccaggctgaa gctgagtacc
     4501 agcccgccga tcacccgata cgggcgaagc cgctcagaca gtgcgggttt ggacttgacc
     4561 gcgacggcga tgccgccttc agactcggcg accggctctg agtgtgtgct ctgtgcgccg
     4621 gagaagaaga tcaccggcaa cacaggcagt atgcatggcg agatgccggt gatcagaccg
     4681 ccgagaaggc cgatcaggac gagtgtgaac atgtcagtta ttcggagccg taacgcattc
     4741 ggatgggtcg agtcgttcgc cgagctgggc ccacgcgcaa gctggggaac gcggaatcgc
     4801 acgaatcccg aaggatccgt gcgattccgc gtcttttcgc actagctggt cggcggcatc
     4861 aggacggtgt cgatcatgta gacctgtgcg ttggcggtcg agacgccgcc gcacaccagc
     4921 ccggcgtcat tgaccttgag gccgttgcct tcaccggtga cagtgacctc gccgccctgc
     4981 acggtattgt gcgctccgac gaccttcgac gggctcgcct ggccgggcac cacgtggtag
     5041 gtcaggatgc tcgtcagcag atccgagtcg gtcttgagct tctctatcgt cgccgggtcg
     5101 atcttcgcga acgcatcgtc ggtcggggcg aacaccgtga actcaccgcc gttgagggtg
     5161 tcgaccaggt tgacgttggg gttgagcttg ccggacaacg cttgggtcag cgtcttgagc
     5221 atcgggttgt tcgacgccgc caccgtcacc ggatcctgcg ccatgccggc caccgagccc
     5281 gggccctcgg gcacctgctc ggcgtaggcc gcacaacccg ttcccaccag attcgccgcc
     5341 gggtccgcct tggcctgcat cgtcgtggcc ggcgcggccg atgacgtcgt ttcggcggcc
     5401 gccggccgtt cagaggtcga gctgtcggaa gaacacgccg aaaggccgag aaccgcgagt
     5461 gccgcgatgg tggccgccgt caatgtgctg cggtgaacat ttctcattgg tgctctgact
     5521 ccttggtccg ggcgggcaag gccgcccatt ggcatgacag cggggattcg gcgtgacagc
     5581 gatcacggat gggcgcggcg ccatggcgtc actcttgtgt catttctcat ggcgagcaga
     5641 cccaaaatcg cgcgtatccg cctcgatctg tgcgagtttc cgtctgctcg gccgagagcg
     5701 gcacaatggg acggtgagca cagcgcacgc gaggagcacg acggacgtcc gccttcgcgt
     5761 gctgatcctc ggcagcaccg gttcgatcgg cacccaggcg ctcgacgtca tcgcggccaa
     5821 cagggatcgc ttcgaggtgg tcggattggc cgcagggggc ggaagccccg acctgctggt
     5881 gcgccagcgc atggaaaccg gcgtcaccaa catcgcagtc gccgatcccg acgccgcgga
     5941 caaagtgggc gacgtcacct atg
//
LOCUS       DASYLX010000742         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3550891,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000742 DASYLX010000000
VERSION     DASYLX010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550891"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1108)
                     /locus_tag="VGP27_17370"
     CDS             complement(170..1108)
                     /locus_tag="VGP27_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEV7853103.1"
                     /translation="MISLRSHAISLAAVFLALAVGVALGSGLLSDTVLSGLRDDKQEL
                     QNQIDGLTDQKNALNEKLSAAGEFDSQMSPRILHDSIAGKSVAVFRTPDAEDDDVDAL
                     TRMVEQAGGTVTGTVSLTEEFVDANSAEKLLSVVNSPIVPAGAQLSTTSVDQGSQAGD
                     LLGITLLINRDPRVPAVNDQQRDTVLTALRDTGFLTYADRVGAANTALLVTGGGLGDD
                     AGNKGATVARFAAGLAPHGSGTVIAGRGGAASGTAAVAVTRSDPALTSSVSTVDDVDA
                     ASGRITTVLALQDLINGGRPGQYGDGQGATSVTVPQ"
     gene            complement(1120..>1814)
                     /gene="steA"
                     /locus_tag="VGP27_17375"
     CDS             complement(1120..>1814)
                     /gene="steA"
                     /locus_tag="VGP27_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEV7853104.1"
                     /translation="LIDGIGIPDIDVDLHRRHVVIVAEEPDAAYDLKALKPFIKEYQP
                     VLIGVGTGADVLRKGGYRPQLIVGDPERISAEVLRSGAQVVLPADADGHAAGLERIQD
                     LGVGAMTFPAAGSAADLALLLADHHGASLIVTAGHTASIEEFFDRSRQHSNPSTFLTR
                     LKVGEKLVDAKAVATLYRSRVSGGAIALLVLAMLIAVIVALWVSRADGAVVDWVVDYW
                     NRFSLWVQSWVT"
BASE COUNT          303 a          602 c          618 g          291 t
ORIGIN      
        1 gcgggtgctt gcgtagcgct ggcaagagca acctccgtga cgacgggcag ggcgtgcaaa
       61 tggctaactg gggcctgccg acccacggaa ctccacatta acaccgacac cgacaagctg
      121 tcgctgacgc gccgtcgcac atccggggag cgagtcgggg atgcgcccgc tattggggga
      181 cagtcaccga cgttgcaccc tgcccgtcgc cgtattggcc gggccgtcca ccgttgatca
      241 ggtcttgcag ggccaggacc gtggtgatcc gcccggacgc ggcgtcgacg tcgtcgaccg
      301 tgctgaccga cgaggtcagc gcggggtcgg acctggtgac ggctaccgca gcggtgccgg
      361 aggcggcccc gccgcggcca gcgatgacgg tgcccgaacc gtggggagca aggcccgcag
      421 cgaagcgggc cacggtggcc cccttgttcc cggcgtcgtc gccgagccct cccccggtga
      481 cgagcagggc ggtgtttgcc gcgccgacgc gatcggcgta ggtgaggaac ccggtgtcgc
      541 gcagcgcggt cagcacggtg tcccgctgct gatcgttcac ggcgggcacc ctggggtccc
      601 ggttgatcaa gagagtgatc cccagcaggt cgcctgcctg ggagccctgg tccaccgatg
      661 tggtgctcag ttgcgccccg gcgggcacga tgggcgagtt caccaccgaa agaagcttct
      721 ccgcggagtt ggcgtcgacg aactcctcgg tcagtgaaac ggtcccggtc accgtgccgc
      781 cggcctgctc caccatgcgg gtgagtgcgt cgacatcgtc atcctcggcg tccggtgtgc
      841 gaaacaccgc cacggacttg cccgctatcg agtcatgcag aatccgcggt gacatctgcg
      901 agtcgaattc accggcggcg ctgagcttct cgttcagtgc attcttctga tcggtcaggc
      961 cgtctatctg gttctgcaat tcctgcttgt cgtcgcgcag gccggacagc accgtgtcgg
     1021 acagcagtcc ggagcccagg gcgacgccga cggcgagggc gagaaagacc gcggcaagcg
     1081 aaatcgcgtg tgagcgtagt gagatcacgc gtcaagctcc taagtaaccc agctctgaac
     1141 ccacagggag aaccgattcc agtagtcgac gacccagtcg accaccgcgc cgtcggcgcg
     1201 cgacacccac agcgcgacga tcactgcgat gagcatcgcg agcaccagca gggcgatggc
     1261 tccgcccgag accctgcttc ggtaaagagt ggcaacagct ttggcgtcca ccagcttctc
     1321 gcccaccttc aggcgcgtca ggaaagtcga gggattgctg tgctggcgcg agcggtcgaa
     1381 gaactcctcg atgctggcgg tgtggccggc ggtgacgatc agcgaggcgc cgtggtgatc
     1441 tgccagcagc agcgcgaggt cggcggccga tcccgcggcc gggaacgtca tcgccccgac
     1501 cccgaggtcc tggatgcgct cgaggcccgc cgcgtgaccg tccgcgtccg cgggcagcac
     1561 cacctgcgcg ccgctgcgca gcacctcggc gctgatacgt tcggggtcac ccacgatcag
     1621 ttgtgggcgg tagccgccct tgcgtagcac gtccgccccg gtgccgacgc cgatgaggac
     1681 cggctgatac tccttgatga acggcttcag tgccttcagg tcgtaggccg cgtcgggttc
     1741 ttccgccacg atcacgacat gacggcgatg aagatcgaca tcgatatcgg ggatgccgat
     1801 gccgtcgatg agca
//
LOCUS       DASYLX010000743         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3660673,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000743 DASYLX010000000
VERSION     DASYLX010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3660673"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGP27_17380"
     CDS             <1..769
                     /locus_tag="VGP27_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV7853105.1"
                     /translation="VGDLALSLVGAGVTADEFAADPARFDIPDSVIEFLRGELGDPPG
                     GWPEPLRTKALAGRPPAKPAEQLTADDEAALALAGAKRQAGLNRLLFPGPTKEFDAHR
                     DLYGDTSGLSANQFFYGLRSGDEHRVTLERGVELLIGLEAISEPDERGMRTVMCILNG
                     QLRPVLVRDRSIAGEVPAAEKADRSNPDHVAAPFAGVVTVAVSSGDKVDAGQTIATIE
                     AMKMEAAITAPKAGTVDRIAVSQTAQVEGGDLLVVVS"
     gene            766..1359
                     /gene="rsmD"
                     /locus_tag="VGP27_17385"
     CDS             766..1359
                     /gene="rsmD"
                     /locus_tag="VGP27_17385"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV7853106.1"
                     /translation="MTRIVAGDLGGRRIAVPPRGTRPTTDRVRESLFNVLTARMDLHG
                     MAVLDLYAGSGALGLEALSRGAATAVFVESDQRAAAVIGQNIKTLGVTGATLRRGTVA
                     SVLATGAQAAVDLVFADPPYDVGEAELNDMLAALSAHGWITAGSVVVVERGASGQQLS
                     WPDGWQRWQARRYGDTRLEMAVVDVAASAAPNGPELP"
     gene            1374..1850
                     /gene="coaD"
                     /locus_tag="VGP27_17390"
     CDS             1374..1850
                     /gene="coaD"
                     /locus_tag="VGP27_17390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV7853107.1"
                     /translation="MSGAVCPGSFDPVTLGHIDIFERAAAQFDEVVVAVLVNPNKKGM
                     FDVDERIAMIEESTEHLQNLRAEAGEGLVVDFVKARGLTAIVKGLRTGTDFEYELQMA
                     QMNKHIAGVDTFFVATTPTYSFVSSSLAKEVASLGGNVSELLPEAVNVRLRDKLKG"
     gene            1953..>2104
                     /locus_tag="VGP27_17395"
     CDS             1953..>2104
                     /locus_tag="VGP27_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV7853108.1"
                     /translation="MVETFVQSTDDAVRFLTDQHNLIKDLFEEVFAASSDEGRDKAFT
                     DLRQLLA"
BASE COUNT          347 a          698 c          721 g          338 t
ORIGIN      
        1 ggtcggtgac ctcgctctgt cgttggtcgg ggcgggtgtc accgccgacg agttcgccgc
       61 cgaccctgcg cggttcgaca ttccggactc ggtcatcgag tttctgcgcg gcgagttggg
      121 cgatccgcca ggcggctggc cggaacccct tcgcaccaag gcgctcgcag ggcggccgcc
      181 tgcgaagccg gctgagcaac tcacggccga cgacgaggcg gcgctcgcgc tggcgggtgc
      241 caagcgtcag gcgggactga accgcctgct gttccccggt cccacaaagg aattcgacgc
      301 gcaccgcgat ctctacggag acacctcggg cctgtcggcc aaccagttct tctacggact
      361 gcgcagcggc gacgagcacc gggtgacgct cgagcggggg gttgagctgc ttatcgggct
      421 cgaggccatc tccgaacccg acgaacgcgg tatgcgcacc gtgatgtgca tcctcaacgg
      481 ccagctgcgc ccggtgctgg tgcgcgaccg cagcatcgcc ggcgaggtgc ctgccgcgga
      541 gaaggccgac cggtccaacc ccgaccatgt ggccgcaccg ttcgcaggcg tcgtcaccgt
      601 cgccgtctcc tccggcgaca aggtcgatgc gggccagacc atcgccacca tcgaggcgat
      661 gaagatggag gccgccatca ccgctcccaa ggcgggcact gtcgaccgga tcgccgtatc
      721 gcagaccgcg caggttgagg gtggtgacct cctggtggtg gtgagctgac gcgcatcgtc
      781 gccggcgacc tcggcgggcg gcgtatcgcg gtgccaccgc gcggcacccg gcccaccacc
      841 gaccgggttc gcgagtcgct gttcaacgtg ctgaccgccc gcatggatct gcacggcatg
      901 gccgtcctcg atctgtacgc aggctcggga gcgctcgggc tggaggcgct ctcgcgcggc
      961 gcggccaccg cggtgttcgt cgagtccgat cagcgggccg cggccgtgat cggccagaac
     1021 atcaagacgc tcggcgtcac gggagcgacg cttcgccgcg gcaccgttgc gtcggtgctg
     1081 gccacgggcg cccaggccgc cgtcgacctg gtcttcgccg atccgccgta cgacgtcggc
     1141 gaggccgagc tgaatgacat gctcgccgca ctttccgcgc acggctggat taccgcgggc
     1201 agcgtcgtcg tcgtggagcg cggtgcgtcc gggcaacagc tgagctggcc ggacggctgg
     1261 cagcgatggc aggcccgccg ctacggcgac acccgcctcg agatggccgt cgtcgatgtc
     1321 gccgccagcg cggctccgaa cggacctgaa ctgccctgat agcgtcgtga tccatgagcg
     1381 gcgcggtatg cccgggatcc ttcgatccgg tgacgcttgg ccacatcgac atcttcgagc
     1441 gcgcggcagc ccagttcgac gaagtcgtgg tcgccgtgct ggtcaacccg aacaagaagg
     1501 gcatgttcga cgtcgacgaa cgcatcgcga tgatcgagga gtcgacggag catctgcaga
     1561 accttcgggc ggaggctgga gagggcctgg tcgtcgactt cgtgaaggcc cgcgggttga
     1621 ccgcgatcgt gaaggggctg cgcaccggca ccgacttcga atacgagctg cagatggcgc
     1681 agatgaacaa acacatcgcg ggggtggaca cgttcttcgt cgccaccacg ccgacgtact
     1741 cgttcgtatc gtcgtcgctg gccaaggagg tggcatcgct tggcggtaac gtctccgagc
     1801 tgctgcccga ggcggtcaac gtgcggctgc gcgacaagct caagggttaa tcgaagggtt
     1861 aatctccggc gtcctcgggt agccggtaga tggcgcgaca gcccgtcggc gcgcccaaaa
     1921 caagctcagg acaattcgga ggtgtatttc ctatggtcga gacattcgtc cagtcaaccg
     1981 atgacgcagt cagattcctc accgatcagc acaacctgat caaggatctg ttcgaagaag
     2041 tgttcgccgc atccagcgac gagggacgcg acaaggcgtt caccgacctt cgccagctgc
     2101 tggc
//
LOCUS       DASYLX010000744        24908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_479377,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000744 DASYLX010000000
VERSION     DASYLX010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24908
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479377"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VGP27_17400"
     CDS             complement(<1..1609)
                     /locus_tag="VGP27_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEV7853109.1"
                     /translation="MSIAPTDTPRLANRAERRVYQALLEQLQPNDLVVAGQRVTDHLK
                     DHEVDFVVAIEGAGIVCLEVKGGEVWHDGHGWRQKRGGRDHSIEPVRQAREACYALRD
                     YIEKDPRWSQGRLRWDHVVVLPNTELPDDFALSDCPRWKVIDRNDLPTMVAKLRDVLV
                     RQQLDRPLLTKHGIDQITTALSGRGLPQRDVVARALANEDAADALTEHQGVILDAIRL
                     LNRIEVRGGAGSGKTFLAMEQARRLAQRGQRVALVCYSHGLASYLERVTATWPRRQRP
                     AYVGEFHALGVQWGAPQGPDESLRTEETVQFWEHDLPRQMAQLAMELGPGHRFDSIVV
                     DEAQDFADSWWEPMLIALKDPDEGGLYVFTDEGQRVFNRYGSPPVPLVPLILDHNLRN
                     TRQIANAFQPLVDHPMRFLGGEGPAVSFVACNRDDALSAGDDQIEALLEQGWRPEDLA
                     LLTTGGRHPEQTERQKEGHTAYWDTFWDADQVFYGHVLGFKGLERRAVVLVVNEASQF
                     DRSRERLYVGLSRARDQLVVCGDPEFIR"
     gene            1779..2120
                     /locus_tag="VGP27_17405"
     CDS             1779..2120
                     /locus_tag="VGP27_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7853110.1"
                     /translation="MRGRGGIAVVLTVCGAVALSGPAQADEYDFISQLDSTGVYYESM
                     LDMIDIGKELCHDLRGGVTPPVVLGKLQNTGFAAAESAIVLMSAVNNMCSDAKAAVVG
                     WARDNGYSGPA"
     gene            2133..2420
                     /locus_tag="VGP27_17410"
     CDS             2133..2420
                     /locus_tag="VGP27_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853111.1"
                     /translation="MQTKTFDVIDLINLVAADHDWDLSFDSGPGDTRELIFSRTNRFD
                     TGLEVEIVFDFTGRIERSEYRRDGELFHRNAVDKWTSAADVHTLTLGLFKR"
     gene            2562..3059
                     /locus_tag="VGP27_17415"
     CDS             2562..3059
                     /locus_tag="VGP27_17415"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7853112.1"
                     /translation="MTGNVRLTPVDERTLEPLLCVAVTEAEPDEVMPPVDAPTGWSHA
                     RREAFRRFHRSNYGGLDGPAHTMMYAIVRDGDVVGMIRMARRDEPDTVETGMWLGRSV
                     RGQGIGVAALRLLLGEAAQAGACRVVAETTTQNTGALWVLRRCGAVLRDDGAGVRAEI
                     ALVGH"
     gene            complement(3091..4128)
                     /locus_tag="VGP27_17420"
     CDS             complement(3091..4128)
                     /locus_tag="VGP27_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7853113.1"
                     /translation="MSGRARWWSLIFFAIIVVALRGPLQGDIGLRAFESDMVEPGLVL
                     VNTDLGLQGVVGAGTGIVLSPDGEVLTNNHVVEGATQITATDSATGRTYPATVVGYDR
                     KHDMAVLQLQGASGLPTAPLGNSSQVRVGEPVLAIGNAGGKGLSRANGTVTAMGQTIV
                     ATDKLAASEQLTGMIETNADIQPGDSGGPLVNDKGQIVGIDTAASDSYRVSSHSGAEG
                     FAIPIDTALSIARQIESGSSAGTIHVGDSPMLGVGVGPGLRAGGVPVRQVLDGSPADQ
                     VGLAPGDVITGFDGKTVNSDTTLTDLLDQHYPGNVVSVTWLDQVGQPHNATVPLGAAP
                     SADSPPPAPLR"
     gene            4377..4835
                     /locus_tag="VGP27_17425"
     CDS             4377..4835
                     /locus_tag="VGP27_17425"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEV7853114.1"
                     /translation="MVLRPGPIADFYREVMGVLDELRLSTEIWPMPVEIEEAIPFGTD
                     KSHVAHHGGVMWEAYSHEVSSAGYWRGLDGEGVFYSYDCPEPPEYRDASVSAAPASFD
                     DGLGELTLPYTAVREADDPDALLLEFLQSTYDVAADTGNWNRAALERSLS"
     gene            complement(4901..5149)
                     /locus_tag="VGP27_17430"
     CDS             complement(4901..5149)
                     /locus_tag="VGP27_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853115.1"
                     /translation="MVKTHAEAAQYVKDAGGLKLVVFGADGHEVAAMFVQGSVRYDEE
                     EFTFLAVAEDGRTVGELFVDEMVDYDENIDAFVIRAAY"
     gene            5562..5873
                     /locus_tag="VGP27_17435"
     CDS             5562..5873
                     /locus_tag="VGP27_17435"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEV7853116.1"
                     /translation="MSSLPPAGWYPNPSGAPGQRYFDGRGWTAHHRAAPPQPVAPPAP
                     PPLPMQTSVVITGPNHALHAVLTLLTFWACGGWGWIWLIVALSNRRRVHVVTTPPPWA
                     R"
     gene            complement(5892..6860)
                     /locus_tag="VGP27_17440"
     CDS             complement(5892..6860)
                     /locus_tag="VGP27_17440"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7853117.1"
                     /translation="MNDGGYGGSAGRDDDELFGADPYPVPPVISAPPPPPRHETNTFA
                     TLSVVFAFVFAPVGAVLGHLGLSQIRRTGQRGHDRALIGLTLSYAFIVIAIVAVVIWA
                     VVRTGPGPSSTVASPPARATSPAAPDERLVKAAQLPQLLLSIDEVKQAVNAPALAKVE
                     EGSGLSGDKGINVTPRECMSALFGGGASAYERSSSRAAFTRALSGDGQDGMILLNEAA
                     STFESTAAATNFVSRTVGEWRGCAGKSVTLIVDGNPLVLDVGEPTQNGTVMMLRNTLR
                     GSKSGFSTDRAITSEANVVIDLDAQGFDLDDSLQTIANRIVDRVPS"
     gene            complement(6945..7118)
                     /locus_tag="VGP27_17445"
                     /pseudo
     CDS             complement(6945..7118)
                     /locus_tag="VGP27_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
     gene            complement(7289..8446)
                     /locus_tag="VGP27_17450"
     CDS             complement(7289..8446)
                     /locus_tag="VGP27_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853118.1"
                     /translation="MTKCPRCFSVLNSEQHLWMVPPRAGATRYFDEVGSAFNGAPAES
                     GPFYKWDRPAGYRGPPPPAAEASRALQGPVVEVCPVCHYPLPEGWHDGHAVCIAMAGA
                     RATGKSLYIAVLVKQLQLLCEKLGVSMEPATRATATAYSTHYETPLFVQRGLIPPTPT
                     VHTQASLQRQPLIFSIGIWHDVRRYLVLRDVAGEDLETGDLSAPPFRFFVNADAVFFM
                     FDPLRVKSIRDQLQDLLPAQTVSGGEPRSVLNNVLLAIGHGHPRLAVILSKFDALRIL
                     RDVQGSEWSLIMSNAGAAYLRDTSDWPSYDEAGGQLLHEEVRSLLTRLNAGTMVAAVE
                     NPSTGKRLTHRYFVVSSLGQPPSGSRLHPRGIAPFRCADPVRWITSGFGVL"
     gene            complement(8443..9351)
                     /locus_tag="VGP27_17455"
     CDS             complement(8443..9351)
                     /locus_tag="VGP27_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853119.1"
                     /translation="MSIPNEALAVSITGSVAEAELDVRGSAQQLVVRPTAHRAVLPEI
                     AGEVTVGVRPVGTARFGAGTVVHLEIAHDDPTEVDPVQVLFDPVDVSGESGVVFAALR
                     PQGSHVEVGVIAVADVPLSPLGSAARSSARQLIGRTPAQSDESVVVALDVTASMRPWF
                     ADGSAAAVTDIVVGVAAAAGIGNVSSVFVGAEVTPVVVGGSDARGTDSVSLADAVRKA
                     RPRWCAGARWLQLAPGPRTIACADFPTSAVQHRFPMIILSNDRRFDAAGARLPAPRQG
                     RDASAELLEHPQVLDRITGFLVGALT"
     gene            complement(9387..11879)
                     /locus_tag="VGP27_17460"
     CDS             complement(9387..11879)
                     /locus_tag="VGP27_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853120.1"
                     /translation="MNTRYGQLAYTSFDPPDSAGGWQIKQVAGALSAGETQTLLAGMR
                     TAFDAVEPLPAYPTPEQLERGPRRLAYRRIDARAAAYWHTVPAGPDSTGRPGNVFAHA
                     ILDRTADAEPLQRPIQWWRSPRWVCPYGAAAVVRAVLPDEVPGPAAAVTKDSVLTFAL
                     DTSTWRLATLFGLLDAVAAALDGGPPVVLGVESTDAAAQWIGLVSFLMSPGTAARLNF
                     STFDRADQVAAALQSKQHLTAVPVVDVTAVPAGVVVIDETATLSLGELGGEPHRTSAG
                     QPIAVTPWSAMAQVVLLEPGSARAVLDEIDRYAAQVEDTALPPAWPMAMAVASAGNYA
                     DAHTEARSVIAAHTPRGVDPASAVGATISGVMTQLVGTRTADAWEAVQHAPAGLAAEH
                     ADLTYACRAITDDTWLSQLGPIPLGPRQFDRSSVPAQLSDAIGPALEQARGVGPEQVL
                     RLVDFLMRAGVDDERLSAALSRDVVPSLTDPHSGPRLVHRLGQRIGTQARLALAAESL
                     EGTPGCTQLDEDVLGWLAENIAAPAPSELAQACPWDAAWTRAALRGARAQYAGAADAA
                     DRWLLLWWLRICGSPRFEQMAAAEVWEPAALLAAAGGAPPGLSALPTLMGAPDSEALR
                     ELASAVTEANADDTAVACAAVRLLEPRMWVNEGRIETYLSTFSAHWDAALSVAHDRLH
                     PDFTVRLLTLAVLATITGLPYPAGCAALAADPALGAQAVGQVVALTEHHVVFAPAVLA
                     AGLVRSAADEQSATSAGGVDGLLGRAARQLAATRVFTDREIDDAAILMAKMSGAEPDS
                     SSLRRNRKLVAKLLAHRAQAQPSLAARTTERR"
     gene            complement(11876..13906)
                     /locus_tag="VGP27_17465"
     CDS             complement(11876..13906)
                     /locus_tag="VGP27_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853121.1"
                     /translation="MPDPQYIDATDDPRLPALMAWRQRLIDAGAVSRTSFKEAHLRLV
                     LRSGRTDVAQIRAMLPPSVAEHAEDMARLLVELENQSGTGEAARRAGVVAADAPRTDA
                     IDVELHYRAGDFAPFRLGEQIGDLHPIGLRRRREEGRSGALELTWPPYPPAGDDSDTV
                     VIYRVVSDEDDPPYSPDRAHLVDATAATSALDDRPPRGAVRHYQVWVNSGPTRGQALG
                     AQPVKHAEAALVSPVRDFAIREDSGQVIGRWSVPSAVSEVFIYRIPAQEAGREGLEHR
                     ILRHSDTRRGFIDTEALRGQRYIYRVRCAVTVGGVTRLSEASEATVEVSAVLAPVTDL
                     SLGAHSPDGSRFELTWTPPPTGAVVIYRSQTAPSAGAEIVELPEATLEQIGLDPVQRL
                     TQPVSQRHDEHGRHRAVMSGVSWPERWSRAYFTPVTLLAGRALPGTTVSAVRTGVIHD
                     VNLAEYCNKQVLTFDWPAGAAAVVTHLAPKGHDPRNGLTGKSFEISLEEYEKYGGMQL
                     SRQELPAAGCSLHLAPVAFSAGRRVVGAIRSVEYAGLLRLQYAVRIARDPDGRPTHAT
                     VALRSQYELPGSPAFVLINNAQRIPLSMHDGQAVDAAPVNEHGQVIGEVSKELRWSAL
                     TTHGVDEMWAANVHGLHGWIRLFVNTPSPARLRTIALLDPSVENLRLLAVAP"
     gene            complement(13909..16560)
                     /locus_tag="VGP27_17470"
     CDS             complement(13909..16560)
                     /locus_tag="VGP27_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853122.1"
                     /translation="MSMPSTSQALTVLVAPHARTEEIFAVLADYSAVGLLAAFTWVDA
                     ADIGGASTPASVLRDGRAEPAVLQQLLTAKRYQRIRVVVLVPVEAPSTERVPLVAEQT
                     VEQIVRSSSMGARISLLRVLLTRGDDAAAAPDSTMVLEGWHNLLVAPEDSAGPGLGAA
                     PSGPITDPLDFAQRTAPVIAGVVGLWAGLEQTPFDTLEILPGQTVRAVRAFYRRLDSS
                     EVEERLRAQLFDPAGRLPLPRGGEVPVVYVDDVAAATQTMARGLWTKHRDLLRGPRLS
                     VSDSGAQAISIWAALKMFLKFMGAALRQAPGAWWSAVTGSVSSVLASTVQGTVFGGRD
                     SAFAVVTNTDVANWQHLGRSADTLNTALGGAPPQHLAQRDLSALWMDFVAGALTLADG
                     GRRAAGLDPVPVGSGVGVLAVASDVVPSAAESFSSIPTSLSAVIGVSSVEPADVLGVA
                     DLRERLQRAYSDPAAGVESRTAGTELEGWQRFVSKSYAWQVGSILADFLHRARGEVAR
                     LVGEIQQATNRVSADARLQARQRAIGVILKTFFWALVLILVVLAGIAAAGRVRWPFAL
                     WTGGVMLGLYILASLALFLFSQRDLFAETHLRQSQLGELETMQANLRTALQDVSRLST
                     AYGQLLSWCRVLGAVLRAPFGPAPAARPSPGQLFDGLPRSTQLGVADPDALQAGDAAH
                     AIQRRLYALGWLTQPWRDMINEVAAKLREEPEMLFRMPGIGTQSGLDQWSSAVASGQV
                     QPTGADALWGRVEQMFAEGQGEVADALTGTVLIPGEDRHAPARQFGAGITEQGDGRAA
                     PFDASLFTDAAMTAGRASVAVDEATVARAGLGYRAAVVQASDGLPPYDFALFAPRVDV
                     AADGEQDATSPDGRERRDVPPRQDMVF"
     gene            complement(16562..20107)
                     /locus_tag="VGP27_17475"
     CDS             complement(16562..20107)
                     /locus_tag="VGP27_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HEV7853123.1"
                     /translation="MRRFLIVGCGGSGGATLAFIMDQLRSELHSAGIETLLGGWQFVQ
                     IDVPSAPETGPEGLANVPEQGGTYIGCGPQGSSYAILDGALSQRLAAESALDTIATWA
                     PRNAQEVSIPISAGAGQYRAIGRMITLSKATEIHTRLQAAWDALFRVDTISQMSTADV
                     PGMGKFDPNEPPLVLVVSSMAGGAGASMALDVCRLLTLVSGLDPKLMGVFMVTPDIFD
                     SLPQSSIVGVRANSLAMLGEIVASQAGSAREHDVRLMRALGHQHGEGELIPFARVFPV
                     GRFIGADRTLFGDGSQWAVYRGLARGLAGLMMSGAASDQFVSYDLGNTASPEGDRDLL
                     GWGISAWDVLPWGTYGFASLSMGRDRYAEYAAQRLARSCVDKLLFGHLQAGNPASSTE
                     QLDSLLSSQWQAHCGELGLPANPGDEQTRTGILGRWIGSDAFAAESVAATVNGLIDRQ
                     LRGYLPNPEGMTAEQWVPIFRQALRNRGSELLRACDDAAYAMAFNWHREFAGRVEQMV
                     GGTMATMGLPYARELVDRLRRHIDDVLAPGMAQLGAMGPSDIVAVPPQVDAALRSMRG
                     VMTNADQVLATALDGFRAGVRRQLFADAAARIADVMRVVGAEMLRPLREALGEAMVLL
                     EQARAEPPTDIGLARLTTDQYAAWPADADELVPARFAEANNEVLLISSTAFQGRYELD
                     LPKAVAGANAMVPLRTAIDDATTQVILGQWRTTGGVSAPGGLTERTATWVTRALGTDP
                     ETSRPRVPSMAQFDVHTRPAELLRRARLYVGRPGESFDEFCKVSLRDFVQGAGAAESE
                     LVTRRRDITTKFAEALSLARPLASVNDEALQRVHPGQQTEYRYKFSEVPFAGQPVADA
                     LFDVLRTNPRIDQATKDNYRRSLSDEDSVTRVDIFGSYPNYSPLAFDSVLKPAAQQWA
                     RIAGPGRGTFWRYRRARPLPASLPMTDDERRAMTAGWFLGQIVGRIHIPEPPYTDPVR
                     IYDADAGHWLNFPSPLLTPPSAFIATYDWLPAVLESILIAIAQSHEPPVMHTLRPYGV
                     LRELYDAHSQDPASGIVELSASDVLREFLVGNAAVPGVTPRIETIAAARSPEERAAAA
                     QEWLTNVRDVAAQYLPGDMPGAAPGGVFTSVPTRSTASKTPIFRDLAPDVFWATEALT
                     RLLQREAAGLARGAATAGTGYLDDGPLVIPEGGTF"
     gene            complement(20108..22792)
                     /locus_tag="VGP27_17480"
     CDS             complement(20108..22792)
                     /locus_tag="VGP27_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEV7853124.1"
                     /translation="MSVAPRILLPVKQIGLLAQFFVAALMMVALAGWSIPSAHAEPAG
                     GGAKRFGACLASQKAGDLVLLFDESSSLQGTDPHAARVQAARYLLETLGRYADRVGAN
                     LEVATSGFSDTYTREQDWTKLTGSTADQVGAQLSSIATKNTGIDTDYWLALDGARQEL
                     ASRGQGSNGGQRCQAIAWFSDGKIDFTSRATSKPYADGITLDSPSGVEDTTKRAVESI
                     CRPGGVADQLRSSSIIMLGIGLGANKTPGDFDVMSAISTARGLNGMACGQITDPIPGD
                     FYPVSNIDDMLFGFDALNPDPGTTGQAPVCQLRVCPEARHNFTLDRSIKSVDILGSGG
                     VAGVVPYLISPSGKTLELPKKDGRNTADLDGVPVTYEWRSDSAQTISLQNTGGPQWPG
                     QWAIVYVDTTGQHPDAVSRVSIHITTDIFPALTNADKTPWRSGQVVKGLTFGLVDGSG
                     KPMQPTDLAGTATMSAALAPDGAAPIPLLDSVAKADIGNPVDADLTKVNPGQAKLRMS
                     LVITTAPATDPSGAQIAPGTQLSPQDVEVPVQILPKLGLPVPGERIDFGTVQAAKGAT
                     ASLNITGPGCAWIADTDAAKVVAAPDDIGTPRLSSAANSAQNCAKVQAGQTGQLSVTL
                     RTDRDGHGGLTGTVPIHVAALDNPGDAQVVDVPFVASLIKPLNTTNFVLVFLAALLLG
                     PGIPLALLYAAKWWVSKIPDTPMLADRIPVEVNNDVVLRDGEPFTLVDTDLIRPVPGL
                     AGGARKVSVQGVTLSTTVGRSPFGPGYVTVDADGLVSVGSALPATDRSGLRAVLPLAV
                     HNKWVVLHDPSGPANVAEVLLLVGGRTDTAARERLYEDVGRRLPELLNGLRLRAVEAG
                     LAVSDNEARQAVSPFGEPAKGGSAAHDPDPFDPFREGN"
     gene            complement(22793..23335)
                     /locus_tag="VGP27_17485"
     CDS             complement(22793..23335)
                     /locus_tag="VGP27_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853125.1"
                     /translation="MGQPNPFDFSDFGPPSGSPPPPRTGADSRPRGADFSTGFDPWAN
                     QPKTQPRARRQDASGQSGERDAFGGAITAGTLPTAGPPLKWFAVALVMAAAGVVLALV
                     SATGGGGVIAPALAGWLLAGPLAIGALAVYTRVDTRRRSESIYSAPRWASRLYWAVLV
                     VCLVGIALGAWQIALWAGRQ"
     gene            complement(23391..24077)
                     /locus_tag="VGP27_17490"
     CDS             complement(23391..24077)
                     /locus_tag="VGP27_17490"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEV7853126.1"
                     /translation="MQPSGNPFEPQSVDPFGTPYEGASAPPSAAPFGHGARIGAPYGA
                     PPSADYNTFAVLSPIFAVVVPPAGVVLGHLALPQIRRSGERGRNAAIAGLVIGYLMCL
                     VLIAALIWWLTSDSDSGDATASPSSSAAPYQVPPAPPRPSTVTSVAPPPTVPRIKLDL
                     ATVPIGTCVEIQLRNDEGNDALDLFKVDCAHKEGVYTVTARVPASYECRSVYVAAPPD
                     RSFALCLNPY"
     gene            complement(24195..>24908)
                     /locus_tag="VGP27_17495"
     CDS             complement(24195..>24908)
                     /locus_tag="VGP27_17495"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEV7853127.1"
                     /translation="QMTHGGHGGGQPDPFGSDPFGGPPSTQGYMPPLTPGSPPMTGRV
                     FPPSGRPRGEVNTFATLSIVFAFVFAPVGAALGHVALSQIKRSGQRGRERAIVGLTIS
                     YVIIVLAVIALVIWLLTANASKSPSSPGETTATPTVTPRASPPPPRTTVFTPPPAQRP
                     TVTVEQLRVGDCVEVQQTQPDPTRGPETNVILIFPVRCEVRDGVFRVDQILSTSACPG
                     QTLFNKQETVFACISDFRG"
BASE COUNT         4055 a         8621 c         8330 g         3902 t
ORIGIN      
        1 cacggatgaa ctctggatcg ccgcaaacga ctaactggtc acgggctctt gataatccga
       61 cgtagaggcg ttcgcgggag cgatcgaact gactggcctc gttgacgacc aagacgacag
      121 cgcggcgttc gagtcctttg aacccgagca cgtggccgta gaacacctga tcggcatccc
      181 agaaggtgtc ccagtacgcc gtgtgccctt ccttctgccg ttcggtctgc tcgggatgac
      241 ggccgccggt tgtcagtagc gcgagatctt cagggcgcca accctgttcg agcagtgctt
      301 cgatctggtc gtcgccggcg ctcagtgcgt cgtcgcggtt gcaggcgacg aacgacacgg
      361 ccggcccctc gccgccgagg aaccgcatgg ggtgatcgac cagcggctgg aaggcgttgg
      421 cgatctggcg ggtgttgcgc aggttgtggt cgagaatcag cggaaccagc ggcaccggcg
      481 gcgagccgta ccggttgaag acgcgctggc cctcgtcggt gaacacgtac aggccgccct
      541 cgtcgggatc cttcagcgcg atcagcatgg gttcccacca cgagtcggcg aagtcctgcg
      601 cctcgtcgac gacgatggag tcgaagcggt gtccgggtcc gagttccatt gccagctggg
      661 ccatttgccg cggcaggtca tgctcccaga attgaacggt ctcctcggtg cgcagcgatt
      721 cgtccggccc ctgcggcgct ccccactgca ctccgagggc gtggaactcg ccgacgtaag
      781 cgggccgctg gcggcgtggc caggtggcag tgacgcgttc caggtaggac gcgaggccgt
      841 gggagtagca gacaagggcg acacgctggc ctcgctgcgc cagccgccgc gcctgctcca
      901 tggccaggaa cgtctttccg ctgccggcgc cgccgcggac ttcgatgcgg ttgagcagtc
      961 ggatggcgtc gaggatgacg ccctggtgtt cggtgagcgc atcggcggcg tcctcgttgg
     1021 ccagggctcg ggcgacgacg tcgcgttgcg gcaatcccct gccgctgagg gcggtggtga
     1081 tttggtcgat gccgtgcttt gtgagcagcg gccggtcgag ctgctggcgg acgagaacgt
     1141 cgcgcagctt ggccaccatg gtcggtaagt cgttgcggtc gatgactttc cagcgcgggc
     1201 agtcagacag cgcgaagtcg tcgggtagct cggtgttggg caggacgacg acgtggtccc
     1261 agcgcagccg gccctggctc cagcgagggt ccttctcgat gtagtcgcgc agggcgtagc
     1321 aggcttcgcg ggcctgccgg acgggctcga tggagtgatc gcggccgccg cgtttctgcc
     1381 gccagccgtg gccgtcgtgc cagacctcgc cgcccttgac ctcgaggcag acgatgccgg
     1441 cgccttcgat cgcgacgacg aaatcgacct cgtggtcctt gaggtggtcg gtgacgcgct
     1501 gcccggcgac gacgaggtcg ttgggttgga gttgttcgag cagcgcctgg tagacgcggc
     1561 gctcggcgcg attggcgagt cggggggtat cggtcggggc gatgctcact ggcgcgtcgg
     1621 ctctctggtg cggagccacg gcagcatcaa gaaccctccc ccggtaagca tcgggtcgat
     1681 ggtagcgagg gggccgtccc gtcggttgac gcggccgtca cttccttgtc ggtggccggg
     1741 tttactgtcg gcttctcagc gcgaatctag gggggatcat gcgcggacgt gggggtatcg
     1801 ccgtcgtgct cacggtgtgc ggggcggtag cgttgtcggg tccggctcag gccgacgagt
     1861 acgacttcat tagtcagctc gacagcacgg gggtgtacta cgagtcgatg ctcgacatga
     1921 tcgacatcgg caaggagctc tgtcacgacc tgcggggtgg ggtgacgccg ccggtggtgc
     1981 tgggcaagct gcagaacacc ggcttcgcgg cagccgagtc ggccatcgtg ttgatgtcgg
     2041 cggtgaacaa catgtgctcg gacgccaagg cggcggtcgt cggatgggcg cgcgacaacg
     2101 gctacagtgg cccggcgtaa ggggttggga cgatgcaaac caagacgttc gacgtgatcg
     2161 acttgatcaa tctggtggcc gcggatcatg actgggacct gagctttgac tcaggccccg
     2221 gtgatacccg agagctgatc ttctcccgga ccaaccggtt cgacaccggc ctggaggttg
     2281 agatcgtgtt cgacttcacc gggcgaatcg agcgctcgga gtaccgccgc gatggcgagt
     2341 tgttccaccg caatgccgtg gacaagtgga cctcggctgc cgacgtccac accctgaccc
     2401 tcgggctgtt caagcgatag ggtgctgccc acctctgcgc gacaactgag ggacactacg
     2461 tcgacggaac gggcctttcg gagttccgca tcgcacggct tcggagggcg caaggtgggc
     2521 caaggcgatg agatgacgcc gacttcggca tactgcgcct cgtgacgggc aatgtgcggc
     2581 tgacgccggt tgacgagcgc accctggagc cgctgttgtg cgtggccgtc accgaggcag
     2641 aacccgacga ggtgatgcca cccgtcgacg cgccgacagg ctggtcgcat gcgcgccgag
     2701 aagcctttcg cagatttcac cggtccaatt acggtgggct cgacgggccg gcacacacca
     2761 tgatgtacgc gattgttcgt gacggcgacg tcgtgggcat gatcaggatg gctcgccgtg
     2821 atgagccgga caccgtggaa accgggatgt ggctcgggcg atctgtccgg ggtcagggca
     2881 tcggcgtggc cgcacttcgg ttgctcctcg gcgaggcggc ccaagccggt gcttgccgcg
     2941 tggtggccga gaccaccacg cagaacaccg gggcactgtg ggtgctgcgc cgctgtggtg
     3001 ccgtgttgcg cgacgacggt gcgggagttc gcgctgagat cgcactcgtc gggcactgac
     3061 cggcacgctt ggggtgggtt cgcggggggc tcatcgcagc ggcgcgggtg gtggcgaatc
     3121 agccgacggg gccgccccca gtggcacggt ggcgttgtgc ggctgaccga cttggtccag
     3181 ccaagtcacg ctgacgacgt tgcccggata gtgctggtcc agaagatcgg tcaacgtggt
     3241 gtcggagttg acggtcttcc cgtcgaatcc ggtgatcaca tcgccgggcg cgaggcccac
     3301 ctggtcggcc ggagagccat cgagcacctg acgcaccggc acgccccctg cacgcaggcc
     3361 gggtccgacg cccaccccga gcatcggcga atcccccaca tggattgtgc cggcggagga
     3421 tccggattcg atctgcctgg cgatcgacag cgcggtgtcg atcgggatcg cgaaaccctc
     3481 tgcgccactg tggctcgaga cccggtagct gtccgaggcc gccgtatcga tgccgacgat
     3541 ctgccccttg tcgttcacca agggcccgcc tgagtctccg ggctgtatgt cggcgtttgt
     3601 ttcgatcatg ccggtcagtt gctcggatgc ggccagcttg tcggtggcga cgatcgtctg
     3661 gcccatcgct gtcaccgttc cgttggcgcg gctcaatcct ttcccgccgg cgttgccgat
     3721 ggccaggacc ggctcgccga cgcggacttg cgacgagttg cccagcggcg ccgtcggcag
     3781 cccgctagct ccctgcagtt gcagaacggc catgtcgtgc ttgcggtcgt agccgacgac
     3841 ggtggccggg taggtgcggc cggtggcgct gtccgtcgcg gtgatctgcg tggcgccctc
     3901 gacgacgtgg ttattggtca gcacttcacc gtcggggctc aacacgatgc ccgtgccggc
     3961 gccgaccacg ccttgaagac ctaagtcggt gttgaccagg acgagacccg gttcgaccat
     4021 gtcgctctcg aaggcacgca ggccaatgtc gccttgcagc gggccgcgca gcgcgacaac
     4081 gatgatcgcg aaaaatatga gtgaccacca tcgtgcacgg cccgacatct atctgctccc
     4141 gattcctgct ttgtctgtga acttccgcct caaggttctc tctgtgaact gggaaatgcc
     4201 tgcgagacgg ctgttgcacc cggaagtgct gcttgcgccc gctcgccaag aacggcatga
     4261 tctgcctttg cgctctgggc ccgcgacacc ctccgccgac aaagggtttt cggtcgactt
     4321 caacttcatc gaccaccaac tgatcgtctc caccaccgtc ggaggatcgg cggaccgtgg
     4381 tgttgcgccc aggcccgatc gccgatttct accgcgaggt catgggcgtg ctcgacgaat
     4441 tgagactgtc caccgaaatc tggcccatgc cagtcgagat tgaagaggcg atccccttcg
     4501 gcaccgataa atcacacgtc gcacaccacg gcggcgtcat gtgggaggcc tactcccacg
     4561 aagtcagcag cgcgggctac tggcggggcc tcgacggcga aggcgtcttc tactcctacg
     4621 actgcccgga accacccgag tatcgggatg catccgtctc agcggccccg gcgtcgttcg
     4681 acgacggtct gggtgaattg acgctgccct acaccgccgt acgcgaggcc gacgaccccg
     4741 acgcgctgct gctcgagttc ctacagagca cctacgacgt cgccgccgac acgggcaatt
     4801 ggaaccgcgc cgcgctggaa cgcagtcttt cctgatcgcc gcttcatcgc ctcgttagcg
     4861 cagtgcagga gaacaacatc cgggtgccgg agcgaaaatg ctagtacgcg gcacggatga
     4921 caaaggcgtc tatgttctcg tcgtaatcga ccatttcatc gacgaacagt tccccaaccg
     4981 tgcgaccgtc ctcagcgacc gcgaggaagg tgaactcctc ctcgtcgtac ctcaccgatc
     5041 cttgcacgaa catcgccgcg acctcgtggc catccgctcc gaaaacgaca agcttcaatc
     5101 cgccggcatc cttgacgtac tgcgccgctt cggcatgtgt cttgaccatc gctgggattc
     5161 ctctcgcctg gcggctccgt tcgtcagcct tggctagcac gccgagctct gctcgcggag
     5221 tcgtgtcgga cccggattcg gattcgacga tgccacatgc cgcacgactg gttctcgaaa
     5281 ctcacaggtt tccgagaaga gagctacgag ctcacccgga gccgactcgt cgtggagggc
     5341 gacgagctgg tttcgacggt catcggtaca gcatcggtgc cctcggcgtg ccgacattgg
     5401 cagaacggcg tgcccgagtc gaggtttccc gcgccgaacg aagcgcggtg ctggccaaag
     5461 gtgcacaagg actcggcgtt cactcgtcgg tcggtccagc tagcgttgcg gtagtcgatt
     5521 cagttggctg aaggcaaact gcggtgatag gaggggcgga gatgagttcg ctgccgcctg
     5581 ccggttggta tcccaaccca agcggtgcac ccggacagcg ttacttcgac gggcgcgggt
     5641 ggacggcaca ccatcgcgcc gccccgccac agcccgtggc cccgcccgca ccgccgccgt
     5701 tgcccatgca gaccagcgtt gtgatcaccg gacccaacca cgcattgcac gccgtgttga
     5761 cgctgctgac cttctgggcc tgcggtggct ggggatggat atggctgatc gtcgccctga
     5821 gtaaccgtcg tcgcgtacac gtagtgacca caccgccgcc gtgggcccgg taagtgtcca
     5881 cccaccagcg ctcagctcgg caccctgtcc acgatgcggt tcgctatcgt ctgcaggctg
     5941 tcatcgaggt cgaaaccctg tgcgtccagg tcgatgacga cattggcttc ggatgtgatg
     6001 gcccggtcgg tactgaaacc ggatttgctc ccgcgcagtg tgtttcgcaa catcatgacc
     6061 gtaccgtttt gcgtcggttc gccaacgtcg aggacgagcg gattcccgtc gacgatcagc
     6121 gtcaccgact tgcccgcgca gccgcgccac tcgccaaccg ttcgtgacac gaaattggtt
     6181 gccgcggcgg tactttcaaa cgtcgatgcg gcctcgttca gcaagatcat gccgtcttga
     6241 ccgtcaccgg aaagcgcacg cgtgaaggcc gcgcgagagg agctgcgctc atatgccgac
     6301 gcgcccccac cgaacaacgc gctcatgcac tcgcggggcg tgacgttgat gcccttgtca
     6361 ccgctcaggc ctgacccctc ctcgaccttg gcaagagcgg gcgcgttgac cgcctgcttg
     6421 acttcgtcga tgctgagcaa cagctgcggc aactgggcgg ccttgacgag ccgctcgtcc
     6481 ggtgcggcgg gactcgtcgc acgagcaggg ggcgacgcga ccgtcgagga aggccccggt
     6541 cctgtgcgca ccaccgccca gatgaccacc gcaacgatcg cgatgacgat gaacgcgtag
     6601 gacagcgtca gcccgatcag cgcgcggtcg tggccgcgtt gcccggtgcg ccggatctgc
     6661 gacagcccga gatgacccag caccgcgccc actggggcga acacgaacgc gaacaccacc
     6721 gacagggtgg cgaatgtgtt cgtctcatga cgaggcggcg gtggcggggc cgagatgacc
     6781 ggaggcaccg gatagggatc ggcgccgaac aactcatcgt cgtcgcggcc agccgagccg
     6841 ccatacccgc cgtcgttcat ctgcagcgca cctcacctcg acattagcgg cccgggccgg
     6901 gcgaccgccc gttaccggac ggcaacgccg gcggccgaca cgatcagcgc caccaccgcc
     6961 accgtgatga acacatacga caacgtgacc ccaaccaacg cccggtcccg cccgcgttgt
     7021 ccggtctggt gaatctgcga caacgcaaca tggccgagaa tcaccccagc gggagcgaac
     7081 acgaacgcaa acaccaccga taacgtggcc aatgtatggg tctcctgctg ctgtggtgcc
     7141 ggcatttccg cggcaccggt ggcagcgccg gtgaagggat caaacggatc agcaccgaag
     7201 ggatctgccc tcgacgacgc ggtgggttcc ccgcggtaac cgccgtgcat cattcccgac
     7261 ctcctcgact cccacgcgcc gacacagctc acaacacccc gaaccccgat gtgatccaac
     7321 gtacggggtc cgcgcagcgg aacggggcga tgccgcgcgg gtgcaggcgg ctaccgctgg
     7381 gcggctgccc aagtgaggac accacgaagt accggtgcgt caaccgcttt ccggtggacg
     7441 ggttttcgac ggccgccacc atcgtccccg cattcaaccg cgtcagcagg ctgcggactt
     7501 cttcgtggag cagctgcccg ccggcctcgt cgtaggacgg ccagtcggac gtgtcgcgca
     7561 gatatgccgc gccggcgttg gacatgatca ggctccactc cgagccctgc acatcacgca
     7621 gtatccgcag cgcgtcgaat ttcgacagga tcaccgccag cctcgggtgg ccgtgcccga
     7681 tcgccagcag cacgttgttc agcacactgc gcggctcgcc cccgctaacc gtctgggcgg
     7741 gcagcagatc ttgcagctgg tcgcgaatcg acttgacccg cagcggatcg aacatgaaga
     7801 aaacggcgtc ggcgttgacg aagaatcgaa acggcggcgc gctcaggtcc ccggtctcca
     7861 ggtcctcgcc ggccacatca cgcagcacca ggtagcgccg gacgtcatgc cagatgccga
     7921 tgctgaaaat caggggctgc cgctgcagcg aggcctgcgt gtgcacggtt ggggtgggcg
     7981 ggatgagtcc gcgctgcacg aacagcggcg tctcatagtg cgtggaatac gccgtggcgg
     8041 tggctcgggt cgcgggttcc attgagacgc cgagtttttc gcacagcagc tggagttgtt
     8101 tcaccagcac ggcgatgtag aggctcttgc cggtggcgcg agcgcccgcc atggcgatgc
     8161 acaccgcatg accatcgtgc catccctccg gcaacgggta gtgacacacc gggcacacct
     8221 ccaccaccgg gccttgcagc gcccggctcg cctccgccgc cggcggtggc gggccgcggt
     8281 agcccgcagg ccggtcccac ttgtagaagg gcccgctctc ggcgggcgcg ccattgaacg
     8341 ccgatccgac ctcgtcgaaa tagcgggtgg ccccggctcg cgggggcacc atccacaagt
     8401 gctgctcgga gttcagcacc gaaaagcacc gcgggcactt cgtcacgtca gtgcccccac
     8461 cagaaagccg gtgatgcgat cgaggacctg cgggtgctcc agcaattcgg ccgacgcgtc
     8521 gcggccctgc cggggtgccg gcagccgcgc cccggcagcg tcgaaccggc ggtcattgga
     8581 caggatgatc atcggaaagc gatgctggac agccgaagtc ggaaagtcgg cgcaggcgat
     8641 ggtgcgggga ccaggcgcca gctgcaacca gcgcgcacca gcgcaccagc gcggccgcgc
     8701 cttccgcacc gcgtcggcca ggctcacgct gtcggtgccg cgcgcatcgg acccgccgac
     8761 gaccaccggc gtgacctccg ccccgacgaa caccgacgac acattgccaa tgcctgccgc
     8821 cgcggcgacg ccgaccacga tgtcggtgac cgcggcggcg ctgccgtcgg cgaaccacgg
     8881 ccgcatcgac gccgtaacgt ccagggccac aacaaccgac tcgtcggact gcgccggcgt
     8941 gcgaccgatg agctgtcgcg cggaactgcg cgccgccgac cccaacgggc tcagcggtac
     9001 atctgccacc gcgatgacgc ccacctcgac gtgggatccc tgcggccgca acgctgcgaa
     9061 caccacaccc gactcgccgc tgacgtcgac cgggtcgaag agcacctgta ccggatcgac
     9121 ctcggtgggg tcgtcatgcg cgatctcgag atgaacgacc gtcccagcac cgaatctcgc
     9181 cgtgccgacc ggtcgcacac cgaccgtcac ctcgccggcg atctccggca acaccgcgcg
     9241 atgtgcggtc gggcggacca ccagctgttg cgcactgccc cgtacatcca attccgcttc
     9301 ggcaacggaa ccggtgatcg acaccgcaag cgcctcattc ggaatgctca cacgctgatt
     9361 gcgctcgcca acaacataaa tcggcatcag cgacgctctg tcgtccgggc cgccagtgac
     9421 ggctgcgcct gcgcgcggtg cgccagcagc ttggccacca gctttcggtt tcggcgcagt
     9481 gatgagctgt ccggctcagc gcccgacatc ttcgccatca atatcgcagc atcgtcgatc
     9541 tcccggtcgg tgaagaccct tgtggcggcg agctgtcggg cggctcgccc caacagaccg
     9601 tcgacaccgc cggccgaagt cgctgactgc tcatcggccg cggaacgcac gagaccggcg
     9661 gccagcaccg ccggtgcgaa gacgacgtga tgctcggtca acgccaccac ctgccctacc
     9721 gcctgcgcgc cgagcgccgg gtccgcggcc agcgccgcac atcccgccgg gtacggcaga
     9781 cccgtgatcg tcgccagcac cgccaacgtc aacaggcgca ccgtgaagtc cggatgcaac
     9841 cggtcgtgcg cgaccgaaag cgcggcgtcc cagtgcgcgc tgaaggtgga caagtacgtc
     9901 tcaattcggc cttcgttcac ccacattcgc ggttcgagca gccgcaccgc cgcgcacgcc
     9961 acagcggtat cgtccgcgtt ggcctccgtc actgcagagg ccaactctct cagtgcctcc
    10021 gagtccggtg cgcccatcag ggtcggcagc gcggataggc cgggcggggc gccaccggcg
    10081 gccgccagca gcgctgccgg ttcccacact tctgcggcgg ccatctgctc aaagcgcggt
    10141 gacccacaga tccgcaacca ccacagcaac agccagcggt cggcggcgtc ggccgccccc
    10201 gcatattggg cgcgcgcgcc gcgcagcgcc gcacgagtcc acgcggcgtc ccaagggcag
    10261 gcctgcgcca gctccgatgg agcaggcgca gcgatgttct cggcgagcca gccgagcacg
    10321 tcttcgtcca gctgagtgca cccaggggtg ccctccagcg attcggcggc cagcgccagc
    10381 cgagcctgcg tgccgatgcg ctgcccgagc cgatggacca gccgtggacc agaatgcggg
    10441 tcggtcaggg agggcacgac atcgcggctc agcgccgccg acagccgttc gtcgtccacg
    10501 ccggcgcgca tcaagaaatc gaccagccgc agcacctgtt cggggccgac accacgcgcc
    10561 tgctccaggg ccggcccgat cgcgtcggac agctgggccg gcactgagct gcggtcaaat
    10621 tgcctcggac ccaatgggat tgggcccaat tgactcaacc aggtgtcatc tgtgatggcg
    10681 cggcacgcgt aggtcaggtc ggcatgctct gccgccagac cggcaggtgc gtgctgcacc
    10741 gcctcccagg catcggcggt cctcgtgccg accaactgcg tcatgactcc agaaatggtg
    10801 gcaccgaccg ccgaggcggg atccaccccg cgaggggtgt gcgccgcgat gacgctgcgg
    10861 gcttcggtgt gggcgtcggc gtagttcccg gcgctcgcca ccgccatcgc catcggccag
    10921 gccggtggca gcgcggtgtc ctccacctgc gcggcgtagc ggtcgatctc gtcgagcacg
    10981 gcgcgcgccg agcccggctc gagcagcacc acctgcgcca tcgccgacca cggcgtcacc
    11041 gcaatgggct gcccggccga ggtgcgatgt ggttctcccc cgagctcgcc caacgacaag
    11101 gtcgcggtct catcgatcac cacaaccccg gccggaaccg cagtcacgtc gacgaccggc
    11161 accgcggtca ggtgctgctt gctctgcagc gccgccgcca cctggtcggc gcgatcgaag
    11221 gtggagaagt tcaggcgcgc ggccgtgccg ggcgacatca ggaaactcac caggccgatc
    11281 cactgagcgg cggcgtcggt ggactcgacc cccagcacca ccggcgggcc gccgtcgagc
    11341 gcggcggcga ccgcgtcgag caggccgaac aacgtggcca gccgccatgt gctggtgtcc
    11401 agtgcgaacg tcagcacact gtccttggtc accgccgccg ccgggccggg cacctcatcg
    11461 ggcaagacgg cgcgcaccac cgcggccgcc ccgtacggac acacccatcg cggcgaccgc
    11521 caccactgaa tcggccgttg cagtggctcc gcgtccgcag tgcggtccag gatcgcgtgg
    11581 gcgaacacgt tgccgggccg gccggtgctg tccggtccgg cgggaaccgt atgccagtaa
    11641 gcggccgcgc gggcgtcgat gcgtcgatag gccagccgcc gcggaccccg ctcgagctgt
    11701 tcaggtgtcg ggtaggcggg cagcggttcg acggcatcga acgcggtacg catccccgca
    11761 agcagggttt gcgtttctcc ggcgctcaac gcgccggcga cctgcttgat ctgccagccg
    11821 cctgcggagt cgggcgggtc gaacgacgtg taagccagct ggccgtagcg ggtgttcacg
    11881 gcgccaccgc taagagccgc agattctcga ccgacggatc cagcagcgcg atcgtccgca
    11941 gtcgcgccgg cgaaggggtg ttcacgaaca gtcggatcca gccgtgcaga ccatgcacgt
    12001 tcgccgccca catctcgtcg acgccgtgcg tggtcagcgc cgaccaccgc agttccttcg
    12061 acacctctcc gatgacctga ccgtgctcgt tgacaggcgc cgcgtcgacg gcttgcccgt
    12121 cgtgcatcga cagcgggatg cgttgcgcat tgttgatcaa aacgaacgcc ggagagccgg
    12181 gaagctcgta ctgcgatcgc aacgcgaccg tggcgtgggt aggccggccg tcggggtcgc
    12241 gggcgatccg caccgcgtac tgcaggcgca gcagcccggc gtactccacg ctgcgaatcg
    12301 cgccgacaac gcggcgcccc gccgaaaagg ccaccggggc caggtgcagc gagcacccgg
    12361 cggcaggcaa ctcctgccgg gaaagctgca taccgccgta cttctcgtac tcctccagtg
    12421 agatctcaaa tgacttgccg gtcaacccgt ttcgcggatc gtggcccttg ggggccagat
    12481 gcgtgaccac cgccgccgca ccggcgggcc aatcgaaggt cagcacctgc ttgttgcagt
    12541 attcggccag attgacgtcg tggatgaccc ccgtgcgcac cgcggacacg gtggtgcccg
    12601 gcagcgcgcg tccggccagc agagtcaccg gggtgaaata ggcccggctc cagcgctccg
    12661 gccacgacac ccccgacatc accgcacggt ggcggccgtg ctcgtcgtgg cgctgcgaca
    12721 ccggctgcgt caaccgctgg accgggtcca gcccgatctg ctcgagtgtg gcttcaggca
    12781 actcaacgat ctctgcgccg gcgctgggcg cggtctggct gcggtagatg accactgcgc
    12841 cggtcggcgg cggcgtccac gtcagctcga agcgcgaccc gtccgggctg tgggcgccca
    12901 acgacagatc ggtcaccggc gccagcaccg ccgacacttc gacggtggct tcgctggcct
    12961 ccgacaaccg cgtgacgccg ccgacggtga ccgcgcagcg cacccggtag atgtagcgct
    13021 gcccacgcaa cgcctcggtg tcgatgaacc cgcggcgggt gtcgctgtgc ctcaggatgc
    13081 ggtgctccag tccctcgcgc cctgcctcct gcgccggtat gcgatagatg aacacctcgc
    13141 tcaccgccga cgggacgctc caccggccga tgacctggcc gctgtcctct cgaatcgcga
    13201 agtcgcgcac cgggctcacc agcgcagcct cggcgtgttt gaccggctga gcccccagcg
    13261 cttggccgcg ggtgggtccg ctgttgaccc acacctggta gtggcgcacc gcgccgcgcg
    13321 gcggccggtc gtccagcgcc gacgtagcgg cggtggcgtc gacgagatgc gcccggtccg
    13381 gtgagtacgg cgggtcgtcc tcgtcgctga cgacccggta gatgacgacg gtgtcagagt
    13441 catctccggc gggcgggtac ggcggccacg tcaactcgag ggcaccggac cggccttcct
    13501 cgcgccgccg ccgcagcccg atcgggtgca ggtcaccgat ctgctcaccc aatcggaacg
    13561 gcgcgaaatc gcctgcccgg tagtggagtt ccacgtcaat ggcatcggtc cgaggcgcgt
    13621 cggcggcgac gacacccgcc ctgcgggccg cttcgcctgt gccgctttgg ttttcgagct
    13681 cgaccagcag ccgggccatg tcctcggcgt gctcggccac cgagggcggc agcatggccc
    13741 ggatctgcgc gacgtcggtg cggcccgagc gcagcaccag acgcagatgg gcctccttga
    13801 agctcgtgcg cgacaccgca ccggcgtcga tgagccgctg gcgccaggcc atcagcgccg
    13861 gcagtcgcgg atcgtcggtc gcgtcgatgt actgcgggtc gggcatcgct agaacaccat
    13921 gtcctgacgg ggcgggacgt cgcggcgctc ccgcccgtcg ggcgacgtcg cgtcctgttc
    13981 gccgtccgct gccacgtcga cgcgaggcgc gaacaacgca aagtcgtagg gcggcaaccc
    14041 atcactggcc tgcaccaccg cggcgcgata ccccaagccg gcgcgcgcga ccgtggcctc
    14101 gtcgaccgca acgctcgcgc ggcccgctgt catggcggcg tcggtgaaca gcgacgcgtc
    14161 gaagggggcg gcccgcccgt cgccctgctc cgtgatgcca gcgccgaatt gccgggcagg
    14221 cgcatgccgg tcctcaccgg gtatcagcac cgtgccggtg agcgcatcgg cgacttcgcc
    14281 ctgcccctcg gcgaacatct gctcgactcg cccccacagc gcgtcggcac cggtgggctg
    14341 aacttgcccc gaggcgaccg cagacgacca ctgatccagc ccggattgcg tgccgatgcc
    14401 cggcatccgg aacagcatct cgggttcctc acgtagcttc gctgccacct cgttgatcat
    14461 gtcccgccac ggctgggtca gccagcccaa tgcgtacagg cgccgctgaa tcgcatgcgc
    14521 ggcgtcgccg gcctgcagcg cgtccggatc ggcgacaccg agttgggtcg agcgcggcag
    14581 gccgtcgaag agctggccgg gtgagggtcg cgccgccggc gcgggcccga acggcgcccg
    14641 cagcaccgcc cccagcaccc ggcaccacga caacagctgt ccgtacgcgg tggacagccg
    14701 gctgacgtcc tgcagcgccg tgcgcagatt ggcctgcatc gtctccaact cacccagctg
    14761 agactgtcgt aagtgcgtct ccgcgaacag atctcgctgc gagaacagga acaacgccag
    14821 cgacgccaga atgtacaggc cgagcatcac accgcccgtc cacagcgcga acggccagcg
    14881 gacccggccg gcggcggcga tcccggcgag aacgacgagt atcagaacca gtgcccagaa
    14941 gaacgttttg aggatgacgc cgatcgcccg ctggcgcgcc tgcagtcgcg cgtcggccga
    15001 cacccggttg gtggcctgct ggatctcgcc caccagtcgg gcgacctcgc cgcgggcgcg
    15061 gtgcaagaag tccgccagga tggaacccac ctgccaggca tagcttttcg acacgaaccg
    15121 ttgccagcct tccagttcgg tccccgcagt acgcgactcg acgccggccg cgggatctga
    15181 gtaggcgcgt tgcagccgct cccgcaggtc ggcaacgccg agcacatcgg ccggttccac
    15241 cgagctgacc ccgatgactg cggacagcga tgtgggaatc gacgaaaacg attcagcggc
    15301 actgggaacc acgtcggagg cgacggcgag caccccgacc ccggaaccca ccggcaccgg
    15361 atccaacccc gcggcgcgac ggcccccgtc ggccagggtc agcgccccgg cgacgaaatc
    15421 catccacagc gcgctgaggt cccgctgtgc aagatgttgc ggcggcgctc cgcccagcgc
    15481 ggtgttcagc gtgtcggcgc ttcgacccag gtgctgccag ttggccacat cggtgttggt
    15541 gaccacagcg aaagcggaat ccctcccgcc gaacaccgtg ccctgcaccg tgctggccag
    15601 caccgacgac accgaacccg taacggccga ccaccacgcc ccgggcgcct gccgcagcgc
    15661 ggcgcccatg aacttcaaga acatcttcag tgctgcccag attgaaatgg cttgggcacc
    15721 agaatcactg acagaaagtc gaggcccgcg gagcaggtct cggtgctttg tccacagccc
    15781 cctggccatg gtctgggtgg ccgccgcaac atcgtccaca tagacgacag gcacctcacc
    15841 gccgcggggc agcggaaggc gtccggcagg gtcgaacagc tgggcgcgca ggcgctcttc
    15901 gacctcgctg ctgtccagcc ggcggtagaa cgcccgcacc gcacgcaccg tctgcccggg
    15961 caagatctcc aacgtgtcga acggtgtctg ctccaatcct gcccacagtc cgacgacccc
    16021 ggcgatcacc ggtgcggtgc gttgcgcgaa atcgagcgga tcggtgatcg ggccgctcgg
    16081 cgccgcaccc aggccgggtc ccgccgaatc ctccggggcg acaagcaggt tgtgccatcc
    16141 ctccagcacc atcgtcgaat ccggtgccgc cgctgcgtcg tccccccggg tcagcaagac
    16201 acgcagcagg ctgatacgcg cacccatcga actgctgcgc acgatctgct ccaccgtctg
    16261 ctcggccacc aggggcaccc gctcggtgga cggcgcctcg acgggcacca ggaccaccac
    16321 ccggatccgc tggtagcgct tggcggtgag caactgctgc aacaccgccg gttcggcgcg
    16381 accgtcgcgc agcacgctgg ccggggtgga cgcgccaccg atgtcggcgg cgtcgaccca
    16441 cgtgaacgcg gcgagcagtc cgacagcgga atagtcggcc aggacggcga agatctcctc
    16501 tgtccgggcg tgcggagcga ccaacaccgt cagcgcctgc gaagtgctcg gcatcgacat
    16561 gtcagaacgt tccgccctcc gggatcacca ggggcccgtc gtcgagatag ccggtcccgg
    16621 cggtcgccgc gccccgcgcc agccccgcgg cctcgcgctg caacaaccgg gtgagggctt
    16681 ccgtcgccca gaacacgtca ggagcgagat cgcggaagat cggcgtcttc gacgccgtcg
    16741 accgtgtcgg gaccgacgtg aacactccgc cgggcgccgc accgggcatg tcccccggca
    16801 gatactgcgc ggcgacgtcg cgcacattgg tcagccactc ctgggcggcg gcggcgcgct
    16861 cctcggggct tcgggccgcg gcgatcgtct cgatccgcgg tgtcacgccc ggcaccgcag
    16921 cgttgccgac caggaactcg cgcagcacgt cgctggcaga caactcgacg atgccgctgg
    16981 ccgggtcctg cgaatgtgcg tcgtagagtt cccgcagcac gccgtagggc cgcagcgtgt
    17041 gcatcaccgg cggctcgtgg gactgggcga tggcgatcag gatgctctcc agcaccgcag
    17101 gcagccagtc ataggtggcg atgaacgccg acggcggtgt cagcagcgga ctggggaagt
    17161 tcaaccagtg ccccgcatcg gcgtcgtaga tgcgcaccgg gtcggtgtag gggggctccg
    17221 ggatgtgaat ccgcccgacg atctggccca ggaaccatcc cgccgtcatg gcgcggcgct
    17281 cgtcgtcggt catcggcagt gacgcaggca agggtcgcgc gcggcgatag cgccagaacg
    17341 tcccgcggcc cggcccggcg atccgggccc actgctgagc ggcgggcttg agcaccgagt
    17401 cgaacgccag cggcgagtag ttggggtagg acccgaagat gtccacccgt gtcacgctgt
    17461 cctcatccga cagcgaccgg cggtagttgt ccttggtggc ctggtcgatg cgcggattgg
    17521 tgcgcagcac gtcgaacagc gcgtcggcga cgggctggcc ggcgaagggg acctcggaga
    17581 acttgtagcg gtactccgtc tgctgcccgg ggtgcacccg ttgcagcgct tcgtcattca
    17641 cactggccag cgggcgggcc agcgacagcg cctcggcgaa cttggtggtg atgtctcggc
    17701 ggcgcgtgac caattccgat tccgccgcgc ccgcgccctg cacgaagtcg cgcagcgaca
    17761 ccttgcagaa ctcgtcgaac gattcccccg gcctgcccac atacagccgg gcccgcctca
    17821 gcagctctgc tgggcgggtg tgcacatcga actgcgccat cgacggcacc cggggccgac
    17881 tcgtctcggg gtcggtgccc agggcgcgcg tcacccacgt tgcggtgcgt tcggtgagac
    17941 ctccgggtgc ggacaccccg ccggtggtgc gccactgccc caggatcacc tgggtcgtcg
    18001 cgtcgtcgat tgcggtgcgc agcggcacca ttgcgttggc gcccgccacg gccttgggca
    18061 gatcgagctc gtagcggccc tggaaggcgg tcgagctgat cagcagcact tcgttgttgg
    18121 cctcggcgaa ccgcgcgggc accagctcgt cggcatcggc cggccacgcc gcgtactgat
    18181 cggtggtcag ccgcgcaagc ccgatatcgg tgggcggttc ggcacgcgcc tgctccagca
    18241 gcaccatcgc ttcgcccagc gcttcgcgca gtggccgcaa catttccgcg cccaccaccc
    18301 gcatcacgtc tgcgattcgc gcggcggcgt cggcgaacag ttggcgtcgc acgcctgcgc
    18361 ggaacccgtc cagcgccgtt gccagaacct gatcggcgtt ggtcatcacc ccgcgcatcg
    18421 agcgcagcgc cgcgtccact tgcggcggca ccgcgacgat gtcggacggc cccatcgccc
    18481 cgagctgcgc catcccgggg gccagcacgt cgtcgatgtg gcggcgcaac cggtcgacca
    18541 gttcccgggc atacggcagg cccatcgtcg ccatcgtccc gcccaccatc tgttcgaccc
    18601 gcccggcgaa ctcgcggtgc cagttgaacg ccattgcgta ggccgcgtcg tcgcatgccc
    18661 gcagaagttc gctgccccgg ttgcgaagag cctgacggaa gatcggaacc cactgctctg
    18721 cggtcatgcc ctcggggttc ggcagatacc ctcgcagctg ccggtcgatg agcccgttga
    18781 cggtggcggc caccgactcg gcggcgaagg cgtcggagcc gatccagcgg ccgaggatgc
    18841 ccgtgcgagt ctgctcgtcg ccggggttcg ccggcagccc cagctcgccg cagtgtgcct
    18901 gccactggct gctcaggagc gagtcgagtt gctcggtgct cgacgccggg ttcccggcct
    18961 gcagatgccc gaacagcagt ttgtccacgc agctgcgcgc cagccgttgc gcggcgtact
    19021 cggcgtagcg gtcgcgaccc atgctcaagc tggcgaaacc ataagtgccc cagggcaata
    19081 cgtcccaagc ggagatgccc cagcccagca ggtcgcggtc gccctcgggc gacgccgtgt
    19141 tgcccaggtc gtaggagacg aactggtcac tggccgcgcc gctcatcatc agtccggcca
    19201 ggccccgtgc cagtccccgg tagacggccc actgcgaacc gtctccgaac agcgtgcggt
    19261 ccgcgccgat gaagcggccc accgggaaca cccgcgcgaa cgggatcagc tccccttcgc
    19321 cgtgctggtg gcccagcgcc cgcatcagcc gcacatcgtg ttcgcgcgcc gagcccgcct
    19381 gactggccac gatctcccca agcatggcca gcgaattggc gcgcaccccg acgatggagc
    19441 tctgcggcag cgaatcgaag atgtcgggcg tcaccatgaa gacgcccatc agtttcgggt
    19501 caagacccga caccagggtc agcagccggc acacatccag cgccatcgac gcccccgcgc
    19561 cgccggccat cgacgacacc accagaacca gcggcggttc gttggggtcg aatttcccca
    19621 tgcccggcac gtcggcggtg gacatctgcg agatcgtgtc gacccggaaa agcgcatccc
    19681 acgcggcctg caggcgggtg tggatctcgg tggccttgct cagcgtgatc atccgcccga
    19741 tggcccggta ctggccggcg cccgccgaga tcgggatgga cacctcctgc gcgttacgcg
    19801 gcgcccaggt ggcgatggtg tccaacgccg attcggcggc cagccgctgc gacaaggcgc
    19861 cgtcgaggat cgcgtagcta ctgccctgcg gtccacagcc gatgtaggtg ccgccctgct
    19921 cgggaacgtt ggccagtccc tcagggccgg tctccggcgc gcttgggacg tcgatctgaa
    19981 cgaattgcca accgcccagg agtgtttcga tgcccgcgga gtgcagttcg gagcgcagct
    20041 ggtccatgat gaacgccagc gtggcaccgc cggaaccacc gcatcccact atgaggaatc
    20101 gtcgcattca gtttccttcc ctgaacgggt cgaatggatc tgggtcatgg gccgccgacc
    20161 cacccttcgc aggttcaccg aacggcgaaa ctgcttgtcg cgcttcgttg tccgagacag
    20221 ccagcccggc ttcaaccgcc cgcaaccgca gcccgttgag caactcgggc agtcgacgcc
    20281 ccacgtcttc gtagagccgc tcacgtgccg cggtgtcggt gcgtccgccg accagcagca
    20341 gcacctcggc gacgttcgcc ggtccgctgg gatcgtgcag gaccacccac ttgttgtgca
    20401 cggccagcgg tagcaccgcg cgcagtccgc tgcggtcggt tgcgggcagc gccgaaccga
    20461 cactgaccag accgtcggcg tcgacggtca catagccggg gccgaagggg ctgcggccga
    20521 cggtcgtgct cagcgtgaca ccttgcaccg agactttgcg agcgcccccg gccagaccgg
    20581 gcaccgggcg gatcaggtcg gtgtcgacca gcgtgaaggg ttccccgtcg cgcagtacca
    20641 catcgttgtt cacctcaacc gggattcgat cggccagcat cggcgtgtcg gggatcttgc
    20701 tgacccacca cttcgccgcg tacagcagtg ccaacgggat ccccgggccc agcaggagcg
    20761 cggccaggaa caccagcacg aagttggtgg tgttcaacgg cttgatcagc gacgcgacga
    20821 acggaacgtc gacgacctgg gcatcgcccg ggttgtcaag cgccgcaacg tgtatcggca
    20881 cagttccggt cagcccgccg tgtccgtcgc ggtcggtgcg cagcgtcacc gacaactgcc
    20941 cggtctgccc ggcctgcacc ttcgcacagt tctgcgcgga gttcgcggcg gaggacagcc
    21001 gcggggtgcc gatatcatcg ggtgcggcca ccaccttggc ggcatcggtg tcggcgatcc
    21061 aggcgcagcc cgggccggtg atgttgagcg acgcggtcgc gcccttggcc gcctgcacgg
    21121 tgccgaaatc gatccgctcg cccggcaccg gcaggccgag cttgggcagg atctgcacgg
    21181 ggacttcgac gtcctggggt gacagctggg tgccgggcgc gatctgcgcg cctgatgggt
    21241 cggtggccgg tgcggtggtg atcaccaacg acattcgcag cttcgcctgc ccgggattca
    21301 ccttcgtcag gtcggcgtct accgggttgc cgatgtcggc tttggccacg gagtccagca
    21361 gcgggatggg cgccgcgccg tcgggcgcca gcgccgccga catggttgcg gtccccgcaa
    21421 ggtcggtcgg ctgcatgggc ttgcccgacc cgtcgacgag gccgaacgtc aggcccttga
    21481 ccacctgccc gctgcgccac ggcgttttgt cggcgttggt aagcgccgga aagatgtcgg
    21541 tggtgatgtg gatgctgacc cgcgatacgg cgtcggggtg ctgaccggtg gtgtcgacgt
    21601 agacgatcgc ccactgaccc ggccattgcg gtcccccggt gttctgcagc gaaatggtct
    21661 gcgctgaatc ggatcgccac tcatacgtca ccggcacgcc gtcgaggtcg gcggtgttgc
    21721 ggccgtcctt cttgggcagt tccaatgtct tgcccgacgg cgagatcaga tagggcacca
    21781 caccggccac tccgccggaa ccgaggatgt ccaccgactt gatcgagcgg tccaacgtga
    21841 agttgtgccg cgcctccggg cacacccgca gctggcagac cggggcttgc ccggtggtgc
    21901 ccgggtccgg gttgagcgcg tcgaacccga acagcatgtc gtcgatgttg gagacgggat
    21961 agaagtcacc cggtatcgga tcagtgatct gtccgcaggc catgccgttg agtccgcggg
    22021 ccgtgcttat cgccgacatc acgtcgaagt caccgggtgt cttgttggcg cccaagccga
    22081 ttcccagcat gatgatgctg ctgctgcgca gctgatcggc gacgccgcca ggccggcaga
    22141 tcgactccac ggcccgtttg gtggtgtcct cgacaccgct cgggctgtcg agggtaattc
    22201 cgtctgcgta gggcttggac gtcgcccgcg acgtgaaatc gatcttgccg tcggagaacc
    22261 atgcgatcgc ttggcagcgc tgtccgccgt tggacccttg cccgcgcgac gccaactcct
    22321 ggcgggcccc gtcgagcgcc agccagtagt cggtgtcgat accggtgttc ttggtggcga
    22381 tgctcgacag ctgcgcaccc acctggtcgg ccgtggaccc ggtcagcttc gtccaatcct
    22441 gttcgcgcgt ataggtgtcg gagaacccgg aggtggcgac ctcgagattc gcgccgaccc
    22501 gatcggcgta tctgcccagc gtctctagca ggtagcgcgc cgcctgcacc cgggctgcgt
    22561 ggggatcggt gccctgcagg ctgctggact cgtcgaacaa cagcaccaga tcgccggcct
    22621 tctgcgaggc caggcaggcg ccgaaccgct ttgcgccgcc gccggccggc tcggcgtgcg
    22681 cggacggaat cgaccagccg gccagcgcca ccatcatcaa cgcggcgacg aagaactgcg
    22741 ccagcaggcc aatttgcttg accggcagca ggattcgtgg tgccacagac atctactgcc
    22801 tgcccgccca cagagcgatc tgccaggcac ccaatgcgat gcccaccaga cacacgacca
    22861 gcaccgccca gtacaacctc gacgcccacc gaggcgccga atagatcgac tcgctgcgtc
    22921 ggcgggtgtc gacccgggtg tagaccgcca gtgcgccgat cgcgagtggc ccggccagaa
    22981 gccagcccgc cagcgcgggc gcgatgacgc cgccaccgcc ggtggcgctg acgagagcca
    23041 gtaccacccc ggctgcggcc atcaccagcg ccacggcgaa ccacttcagc ggcggcccgg
    23101 cggtcggcaa cgtccccgcc gtgatcgccc caccgaaggc gtcacgctcg cccgactgac
    23161 cggaggcgtc ctggcgtcgc gcgcgcggtt gtgtcttcgg ctgattggcc cagggatcga
    23221 agccggtgga aaagtcggca ccccgcggtc gcgaatccgc gccggttcgc ggtggcgggg
    23281 gactcccaga aggcgggccg aagtcgctga aatcgaacgg attgggttga cccacgtcga
    23341 tcaccccggc cttcgctggt gggtggtcgc ttcgaggatg ttcatcggca tcagtacggg
    23401 ttcagacaca gggcgaagga gcggtcgggc ggcgcggcga cgtagacgga acggcattcg
    23461 tagctggcgg ggacccgcgc ggtgacggtg tacacccctt ccttgtgcgc gcagtcgacc
    23521 ttgaacagat ccaacgcgtc gttgccttcg tcgttgcgca gctggatctc cacgcaggtg
    23581 ccgatcggca ccgtggcgag gtcgagcttg atccgcggaa cggtgggcgg cggggccacc
    23641 gaggtcaccg tcgagggtcg cggcggagcc ggcggcacct gatagggggc ggcgctcgac
    23701 gagggtgagg cggtcgcgtc gccggagtcc gaatcactcg tcaaccacca gatgagggcc
    23761 gcgatcagca ccaggcacat cagatagccg atcacgagtc cggcgatggc agcgttgcgt
    23821 ccccgctcac cggagcgccg gatctgcggc aaagccaggt gccccagcac caccccggcc
    23881 ggcggtacca ccaccgcgaa aatcggcgac agcaccgcga aggtgttgta gtcggccgac
    23941 ggcggtgctc catagggcgc accgatccga gcgccgtgcc cgaacggagc tgcgctcggt
    24001 ggcgcggacg cgccttcata cggtgtcccg aacggatcca ccgactgcgg ctcgaacgga
    24061 ttaccgctgg gctgcatcag agccccccgt cggcggcggc cttggccgac cgcgagctgc
    24121 aggccagcga cccgcgagca tgcgcccgcg gtaatcgcga ctaaggccag tcagtgaaca
    24181 caacctcatc atcatcagcc tcggaaatcc gagatgcatg caaataccgt ttcttgcttg
    24241 ttgaacagtg tctgcccagg gcatgcactg gtcgacaaga tctggtctac ccggaataca
    24301 ccgtcgcgga cctcgcagcg caccggaaat atgaggatga cgttcgtttc gggccctctg
    24361 gtcgggtccg gctgcgtctg ctggacctcg acgcagtcgc ctacccgcag ctgctccacg
    24421 gtgaccgtcg gccgctgagc cggtggcggc gtgaagaccg tggtgcgcgg gggtggcggg
    24481 gaagcgcgtg gtgtcaccgt cggcgttgct gtcgtctcac cgggcgacga aggggacttg
    24541 gaggcgttgg cggtcagaag ccagatcacc agcgcaatca ccgccagcac gatgatcacg
    24601 taggagatgg tgaggcccac tatcgctcgt tcacgaccgc gttgcccgct ccgtttgatc
    24661 tgtgagagtg ctacgtggcc cagtgccgcg ccgacggggg cgaacacgaa cgcaaacacg
    24721 atggacaagg tcgcgaacgt gttgacttca ccgcgtggcc gacccgacgg cgggaagacc
    24781 cgccctgtca taggcggtga gccgggggtg agcggcggca tgtacccctg ggtgctcggc
    24841 ggaccaccga aggggtccga accgaacggg tcgggctgac cgcccccatg cccgccatgc
    24901 gtcatctg
//
LOCUS       DASYLX010000745         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1466571,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000745 DASYLX010000000
VERSION     DASYLX010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466571"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VGP27_17500"
     CDS             complement(<1..2000)
                     /locus_tag="VGP27_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7853128.1"
                     /translation="MTAALLDLDGHMVDRDDERTDLRDAVDAAGRTGGGCVLLSGAPG
                     VGKSALMQAFGFEITGRNCVFAYGRCREGAPAPYTALAEALRAIVRGMESTGPAERDR
                     WRSDLLSATSALTGILGDLVPELVEVFGETPQPADLDAADARRRLHRAVIRLISDTAA
                     YRPVVLAVDDLQWADRDTLLLLSELLTVSPRNVLVLGAHRAGDFDAAAAGLTSSSVHS
                     IELSPLADKDVEELLASVCGRSVELSHVTTEFQHRTGGNPLQIRQLLYRAQREGALTP
                     AESGGQSNWDLRVLSSIEVTATSAEFLGRYLEQLRPIDREVLSSLACIGGEFDLHDAT
                     SAAAKLADVVAMALWAGLEFRLIEAVDGSGRRIANAISHDAHYRFTHDRVAEAARADL
                     PDDAQREIHLRIARRLVKLGDGRLFEAARHISAGGKALTEDERVDFVQVLHKAARKAR
                     AQASFPLALEYSRTGLRLLGGRRWDTAFSLSRELQLSAAEAAVLVGDVVALHELLDEA
                     DQFLPEPADRARLAYLRLKGRVAENRIQDAIDTGLQALDEFGEPLPHNPGTPRLGIEL
                     VRMKMKMSGWSNERLLALPDCEDPRVIELLRILAEMHSLSYIGRPNLLPLIVRKQLEL
                     TLAHGHTPSLPVVLASYGAVLVFAGDRNGAQRFGEVALQLVER"
     gene            2793..4226
                     /locus_tag="VGP27_17505"
     CDS             2793..4226
                     /locus_tag="VGP27_17505"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HEV7853129.1"
                     /translation="MIIGVMQSLTTAVTASLGLTAFQALIVPGTGTPHAADDYLNNVL
                     NYYVVPGADPACSVTAPAGPCPTTQSIDYFATFWPIPLPGWGGLSGQKWNVSVASGVT
                     GLTKAYEALPSDSDVLIFGYSQGATVASNFKKAHPQDGVEPGDHTTDYFFIGNPQKPN
                     GGFFERFAFLGNVPILDAQFGDPSPTDACNGGDRVCTTDFALQYDGVVDFPQWLANPV
                     ADLNAVAGFAYVHGTYLAPNGDDPVTETPYGYSVKEVQDAVTAAQANCSVETYCQHVD
                     GSDTIYVVLPARYLPIYQPFIDLGDATGTSALVVPVTDFLSPFTQTIIETGYDRTDYS
                     KPTPGTLLPPANFNPVQTAVDLVHDVPEGINMALTPGRTPLPGSPPVTSASTLSDTGG
                     NQLVANSATPKPLDGTPLVRSGLFAKPGGGVTAVGANGSNANRPLSNALKDFHPVRDV
                     VKAVSGEVKKALGKDDSSAAGDNAESN"
     gene            4297..>5051
                     /gene="eccB"
                     /locus_tag="VGP27_17510"
     CDS             4297..>5051
                     /gene="eccB"
                     /locus_tag="VGP27_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEV7853130.1"
                     /translation="MARQPTTRLQVSGYRFLLRRMEHALLRGDIRMLDDPMRTQSLSL
                     IAGCVLAVVVVAVCAVLAFLRPNAGLGDAAIVVSRDSGALYVRIGDTLHPVLNLASAR
                     LATGNGDSPRSVTAADIDNSKRGPLLGIPGAPFAIGQPLSTRESAWTVCDDKTTTVIA
                     GPTDLNRHDHQPNVLVSPRTESAAVTYLLYDGRRAQVDLRNPSVLWALRLDGVEPRPV
                     SRALLDAVPEAPPITAPHIRGAGSPGAIAGLPVG"
BASE COUNT          926 a         1651 c         1550 g          924 t
ORIGIN      
        1 cgttcaacga gctgcaacgc cacctcaccg aagcgctgcg cgccgttgcg atcaccggcg
       61 aacaccaaga ccgccccgta actggcgagc accaccggca agctcggcgt gtggccgtgc
      121 gccaacgtga gctcgagctg cttacgcacg atcagcggta gtaggttcgg ccttccaatg
      181 tacgagaggc tgtgcatctc agcgaggatc cgtaagagct cgatgacgcg gggatcctcg
      241 cagtccggga gtgcgagtag ccgctcgttg ctccagccac tcatcttcat cttcatccgc
      301 accagctcga ttcccagccg cggcgtccct gggttatgtg gcagcggctc gccgaattcg
      361 tcgagtgcct gcagcccggt gtcaatcgca tcctggatac ggttctcagc gacgcgcccc
      421 ttcaatctga gataggcgag acgagcgcgg tcagccggtt cggggaggaa ttggtcggct
      481 tcgtccagca attcatgcag ggctaccacg tcgccgacca gtacggcggc ttccgctgcg
      541 ctgagctgca gttcgcgcga cagcgaaaag gctgtgtccc aacgccttcc gcctagaagt
      601 cgcaagccgg tgcgggagta ctcaagagcc aaggggaacg atgcctgcgc tcgcgccttg
      661 cgtgccgcct tatggagcac ctgaacaaag tcaacgcgtt catcttcggt caaggcctta
      721 ccgccggcgc tgatgtggcg ggccgcctcg aacagtcggc cgtcacccag cttgacgagc
      781 cggcgggcga ttcgcaggtg tatttcgcgc tgggcgtcgt cgggcaggtc ggcccgcgcg
      841 gcctcggcca ctcggtcgtg agtgaaccgg tagtgcgcgt cgtggctgat ggcgttggcg
      901 atgcgccgac cactgccgtc aacggcttcg atcagccgaa actcaagacc cgcccagagt
      961 gccatggcca ccacgtcggc cagtttcgcg gccgctgacg tcgcgtcgtg gaggtcgaat
     1021 tctccaccga tgcacgctaa cgagctcagt acctcacggt cgatgggccg cagttgctcg
     1081 aggtaacggc cgaggaactc ggcggaggtg gcggtgacct cgatagaaga cagaacgcgc
     1141 aggtcccaat tcgactgccc gccggactct gcaggtgtga gtgcgccttc gcgttgtgcg
     1201 cggtacagca gctgacggat ctgcagggga ttgcccccgg tccgatgctg gaattccgtg
     1261 gtcacgtggc tcagttcgac gcttcggccg caaaccgaag caagcagttc ctccacatcc
     1321 ttgtccgcca gcgggctgag ttcgatactg tgcacgctcg acgacgtgag acccgctgca
     1381 gccgcgtcga aatcgcctgc acgatgcgca ccgagaacca gcacattgcg aggcgacacc
     1441 gtcaaaagtt cggacaacag cagcagtgtg tctcgatcgg cccattgcag gtcgtcgacc
     1501 gccaagacca ctggccggta ggccgcagta tcggagatca ggcgaatgac cgcgcggtga
     1561 agtcggcggc gggcgtcggc cgcgtcgagg tctgccggct gcggtgtttc gccgaatact
     1621 tcgaccaatt cgggaacgag atccccgagg atgcctgtca atgccgacgt tgcactcaga
     1681 agatctgagc gccaacggtc gcgctcggcc ggacccgtcg actccatccc gcgaacgatc
     1741 gccctgagcg cttctgccaa agccgtatat ggagcaggcg caccctccct gcacctgccg
     1801 taggcgaaga cgcagttgcg tccggtgatt tcgaagccga aggcttgcat cagcgccgac
     1861 ttgccgacgc caggagcacc gctcagcagg acacatcctc cgccggtgcg tccggccgca
     1921 tccacggcgt ctcggaggtc ggtccgctca tcgtcgcggt cgaccatgtg gccgtcgaga
     1981 tcgagtaacg cggccgtcat cgttctggac gcacttcgac tcgcgccgtc cccccgacgg
     2041 cgcggctatc gaaccggatc tgctcgttca actgcaacat ctcgtctacg acgtcgtcct
     2101 cactatgcgc cgagtgcgta ctcgggttgc tgaaactgca atatctcgcg tcttcgttga
     2161 ggccgggaca tcccttgttc cctacattct ctgtagcacg cgaaggcttg tggcgcccga
     2221 cgccatgtaa gcacgggaaa aactctgacg gaattcactg atggccgtcg gcgactcgcg
     2281 ctgtcgtcag cgcccgtagc aacttccatc atcaacagcc ccccaccact cgagacggta
     2341 tcgagtgctg ataattttcg cacgcaataa tttgctcgtt gggaccttcg acgttcgact
     2401 actgcgctgt gcgcggcgcc gtcgatcgcg ccgagacagt ggattcgctg cgcgaagggt
     2461 gcattgattg ctccagtgcg cgaggatttc ggcctgcctg cgcttcgaag gcgacgtccc
     2521 ggtacagcgc agggcggttt cggaggcacg ccgccttaag gccttgatat tcagcccctc
     2581 agacaccttc tacctaagcg gtcgtcggac tcgacgcgaa ccgatcgtgc aataagcgga
     2641 gttatgccag tttgctcgat atcgccgcgc aaaccgcact ccggtgcgag gatccctcgg
     2701 gaacagaaca gcgaggagag gcgtacatgg gcatgtcggc tcaatcagtg gtgaagacgg
     2761 cggtcgtagc gatcttgacc gcactggccg ccttgatcat cggcgtgatg cagtcgctga
     2821 cgacggcagt caccgcgtcc ctcggattga cggcattcca ggctttgatc gtgcccggta
     2881 ccggcacgcc gcatgcagct gacgactacc tcaacaacgt gctcaactac tacgtcgtcc
     2941 ccggcgctga ccccgcttgt agcgtgaccg ctccggctgg cccgtgtccg accacgcaga
     3001 gcatcgacta cttcgcgacc ttttggccaa taccgctgcc gggatggggc ggcctatcag
     3061 gtcagaagtg gaacgtgtcg gtcgcaagcg gggtgaccgg cctcaccaag gcatatgaag
     3121 cgctcccgag tgatagcgac gtactgatct tcgggtactc gcagggtgcc accgtcgcca
     3181 gcaatttcaa aaaggcgcac ccgcaggacg gcgttgaacc cggcgaccac acaaccgact
     3241 acttcttcat cggcaacccg caaaaaccaa acggcggctt cttcgaaagg ttcgcctttt
     3301 taggcaacgt tcccatattg gatgcgcaat tcggcgaccc atcgccgacg gacgcctgca
     3361 acggcggcga tcgggtctgc acgacggatt tcgcgctgca atacgacggt gtcgtcgact
     3421 tcccacagtg gctggccaat cccgtggccg atctcaatgc ggtcgccggc ttcgcttacg
     3481 tacatggcac gtatcttgcg cccaacggcg acgatccggt taccgagacg ccgtatggct
     3541 acagcgtcaa ggaggtgcag gacgcggtca cggccgcgca ggccaactgc agcgtagaga
     3601 cgtactgcca gcacgttgac ggtagcgaca ccatctacgt ggtgttgccg gccagatatc
     3661 tgccgatcta tcagcctttc attgacttgg gagacgcgac tggcacctca gcgctggtgg
     3721 ttcccgtcac cgattttctt tcaccgttta cccagacgat catcgaaacg ggttatgacc
     3781 gaacggatta cagcaaaccg acaccgggca cactgctccc gcctgccaat ttcaacccgg
     3841 tccagacagc agtcgacctc gtgcatgacg tcccggaggg catcaacatg gcgctgacgc
     3901 ccggccgcac accgctgccg ggatcgcccc cggtgacaag tgcgagcacg ctgtcggaca
     3961 cgggcggcaa ccagctcgtc gccaactcgg cgacaccgaa gcccctcgat ggcacgccgc
     4021 tggttcggtc agggctgttc gccaaacccg gtgggggcgt cacggcagtc ggtgcgaacg
     4081 gatcgaacgc caatcgcccg ctgagcaacg cgctgaaaga cttccatccg gtgcgcgacg
     4141 tcgttaaggc ggtttcgggc gaggtgaaga aggcgctcgg caaggacgac agctctgccg
     4201 ccggcgataa cgccgagtcc aattagtcgc gcggcccgcg tcagattggt gaaaggtcat
     4261 acccccgagc gccattaggc tgggcgccgg gggagtatgg cgcgtcaacc gacgacccgg
     4321 ctgcaagtca gcgggtaccg atttctgctg cggcggatgg aacacgcatt gctgcgcggt
     4381 gacatccgca tgcttgacga tccgatgcgg acacagtcgc tttcgttgat cgcaggctgc
     4441 gtgcttgccg ttgtcgttgt cgccgtgtgc gcggtcctgg cgtttctccg gcccaacgcc
     4501 ggcctgggtg atgccgcgat cgtcgtgtcc cgcgactcgg gagcgctgta tgtccgcatc
     4561 ggcgacacgc tgcaccccgt cttgaatctg gcttcggcgc gcttggccac cggcaacggt
     4621 gatagtccgc gaagcgtcac agcggccgac atcgacaact cgaagcgcgg tccgctactg
     4681 ggcatccccg gtgcgccgtt cgccatcggc cagccattgt ccacccgaga atccgcctgg
     4741 acggtctgcg acgacaagac gacgacggtg atcgccggcc ccacggatct gaatcgacac
     4801 gaccatcaac cgaatgttct ggtcagtccg cgaacggaga gcgcggccgt aacctacctg
     4861 ctgtacgacg gccggcgtgc gcaagtcgat ctccgtaacc ccagtgttct atgggcccta
     4921 cggctcgacg gtgttgagcc gcgacctgta tcgcgggcgc tcctcgacgc cgttcccgaa
     4981 gcgccgccga tcactgcgcc acatatccgc ggtgccggtt ctcctggcgc catcgccggc
     5041 ctgccggtcg g
//
LOCUS       DASYLX010000746         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_809682,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000746 DASYLX010000000
VERSION     DASYLX010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809682"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(509..793)
                     /locus_tag="VGP27_17515"
     CDS             complement(509..793)
                     /locus_tag="VGP27_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853131.1"
                     /translation="MRMKLSYMTPLLGAGAAALAIIAAPIASAADTGQSCGASGTGTV
                     CQSPGNVQINDSPPSVGFYPYGGDAFLLGGYGGGGFHGGGGFHGGGGGHR"
     gene            complement(1286..2026)
                     /locus_tag="VGP27_17520"
     CDS             complement(1286..2026)
                     /locus_tag="VGP27_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV7853132.1"
                     /translation="MTLSDRAELRGEARHPATDPHASHVAKRLNWLRAGVLGANDGIV
                     STAGLVVGVAAATTTSSVIFAAGVAGLVAGAVSMALGEYVSVSTQRDTQRVLLQKERR
                     ELDEMPEAELEELAGLYAAKGLSPETARVVARELTDRDAFAAHVDVELGIDPDDLSNP
                     WQAAFSSAVAFTLGAVIPLIAILLPPVSARVPLAFLSVLIALVLTGTVSAILGGAPKR
                     RAVLRVVLGGALAMLVTYGIGQMVGTTI"
     gene            2463..2825
                     /locus_tag="VGP27_17525"
     CDS             2463..2825
                     /locus_tag="VGP27_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7853133.1"
                     /translation="MRCLIVDDSAVFVGAARGLLECEGITVVGVASTSADALRHFEQL
                     RPDVTLVDINLGSESGFELAEQLHRVGGPSPSPVILMSTHAAQDFADMIDTSPAVGFL
                     SKSALTCGAIHDLAGESA"
     gene            complement(2817..3012)
                     /locus_tag="VGP27_17530"
                     /pseudo
     CDS             complement(2817..3012)
                     /locus_tag="VGP27_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689576.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3320..3606
                     /locus_tag="VGP27_17535"
                     /pseudo
     CDS             3320..3606
                     /locus_tag="VGP27_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(3781..>4663)
                     /locus_tag="VGP27_17540"
     CDS             complement(3781..>4663)
                     /locus_tag="VGP27_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV7853134.1"
                     /translation="DVANARARAVENLERASESMEYFIGIAHAALGTVHLTTGDASAA
                     WEAYEAARERTVMDPMTSGIYTWAPLGPLASGDLAAARRWADDVVSATTNWSLAAALT
                     SRSRVEIAQGELDAADRDAYDALDLAARLGGDLVVPFALDCLAVVAGDTDNFVSSARL
                     FGAADAARQHMGLVRFKIFDEGDEARIAAVRDALGEKDFEAARAEGAALSIEEATAYA
                     LRGRRERKRASSGWASLTRAELDVVKLVSEGLSNKEVAARLFVSPRTVQAHLTHVYTK
                     LGLTSRVQLAQEAAGHA"
BASE COUNT          867 a         1554 c         1422 g          820 t
ORIGIN      
        1 tgggtgatcc ggcgagcctg gtagagcccg ctgctgccgc tcaccgggga caacacgccg
       61 atccagtcga ccaccatccg ggcaattagc acgagttcga acagcaagag aacgattcca
      121 agcaagcctg cgaccaccgc acactccttc cgacttcccc gattctccgc cgtccagctg
      181 agaagggact gagtccgtcg ccgcactgcc ccgcaggccg tgttcgggat cgagtcgctc
      241 gtaatcccac cgaaattggc tctcagcgtg agctcagcaa cgccgctcta ggtggcgcct
      301 ctcctttact ggctggcaca tctataagtc cagaattgcg gcgcggcgaa cgtctgtcat
      361 cgctgctgac agccatcaat tcttcccgcc agctggaata tcttccgtcg cagccgaaaa
      421 gggcgcccgt gtgagaacaa tgtcacaccg acgccccggt cagcacctgg atacggccaa
      481 aacagcttta cctcaacgtc attgaccgtt agcggtgtcc accgccgccg ccgtgaaagc
      541 cgccgccgcc gtgaaagccg ccgccgccgt acccgccgag cagaaaggcg tcaccgccgt
      601 agggatagaa gcctacagac ggaggtgagt cgttgatctg gacgttgccc ggtgactggc
      661 acacggttcc cgtgccgctc gcgccgcagg actgtccggt gtcagcagct gacgcaatcg
      721 gtgcggcaat aatcgcaaga gctgcagcgc ccgcacccaa caacggtgtc atgtaactca
      781 gtttcattcg catggtggcg ttccttcgat ggttggttgt gtcttccgtg ttccaggttt
      841 gtccgccgcc gaacccgcgt cgtcggtgct gacgtctatc agcccgcccg ccaccccgaa
      901 tctctgtcta cccgtcagca ggtattcgcg gcgatgcgcc ggctcggcct acgcccggac
      961 tgcaccaaaa atgatggcgg caccgatatc tggtgatacg gctttcgccg tcgtcatcga
     1021 caccgccagc ccgaaccccc caaccggccg aaaccaccag aacgccgagc aagaaacaac
     1081 accaaagggc accccggagg gatccgctcg agcccgtacg gcagaactgt ctccgcaacg
     1141 atgaaggacg cggcgaccgc gaggccgata gcgaacggta gggccgtggg cgcaccaaca
     1201 ggcggacccg gcgagcgctc aacaatggcg atagtgcccg tacatctgaa cttcgtgcca
     1261 cgcgcacctc aaccacaggt ttggatcaaa tcgtcgttcc gaccatctgt ccgatgccgt
     1321 aggtaacgag catcgcgagc gctccgccaa gcactacccg caagaccgcg cgccttttgg
     1381 gggcaccgcc caggatggcg ctcaccgtcc cggtcaacac cagcgcgatc aacaccgaca
     1441 gaaaggccag ggggacacgg gcgctcacgg gcggcagcag aatcgcgatg agtggaatga
     1501 cggcacccag ggtgaatgcg accgccgacg agaacgctgc ctgccacggg ttggacaaat
     1561 cgtccgggtc gatgccgagt tccacgtcaa catgggcggc gaaggcgtcg cgatccgtca
     1621 gttccctggc gacgacccgt gcggtttccg ggctgagtcc cttcgccgcg taaagccctg
     1681 ctaattcttc aagttcggcc tcgggcatct cgtctagctc tcggcgctcc ttctgcagca
     1741 gcactcgttg cgtatcacgc tgcgtgctca ccgacacata ttcgccgagc gccatcgaga
     1801 ccgcgccggc gaccagaccc gccactccgg cagcgaagat caccgagctg gtcgtcgtag
     1861 ccgcggccac gccgaccaca agacctgccg tggagacgat tccgtcgttc gctccgagca
     1921 cccccgcacg taaccagttc agtcgcttgg cgacgtgcga cgcatggggg tccgtcgcgg
     1981 gatgccgggc ctcaccgcgc aattcagcgc ggtcggaaag cgtcatgtcg cggcgatcga
     2041 caggcgactg ccgggtggcg acgcggttgt ggctgcggcg gtgacgccga gctcgcgagc
     2101 ccgacggatg gcagtcacgg ccgcctcttc gtcgaactgg ccccagtcgc cgccgagtag
     2161 ccatgtccca aaaaggcgat gcgcgagact ttcaggctgc ttcggcccaa tgtggtggtc
     2221 ttcatgctgt tccaatctca tcgagcatcg gcaggcctgc gtctgcgtcg ttgctgtcca
     2281 ggattgcctg cgcgcgagcc ggtgtcgtta atgctgatac caatcggtgc ccctagtagt
     2341 gggaatctgc atctaccggc cagctgtaaa tgctctatat tgtgggcaag attggcagct
     2401 agtaggaatg cgcacttatc cccgcactgc gaagctgatc caatgctgct gcgagtgacc
     2461 cgatgcgctg tctgatcgtc gacgacagcg cggttttcgt cggcgccgcg cgcggcctgc
     2521 tggagtgcga gggcatcact gtcgtcggcg tggcgtcgac gagtgccgac gcgcttcggc
     2581 atttcgagca gttgcggccc gacgtgacgt tggtcgacat caacctcggg agcgagagcg
     2641 gcttcgaact cgccgagcaa ctccaccggg tgggggggcc gtcaccgtca ccggtgatac
     2701 tgatgtccac ccacgccgcg caggacttcg cggacatgat cgacaccagt ccggccgtcg
     2761 ggttcctgtc caaatcggcc ctgacgtgcg gcgccatcca cgatctcgcc ggcgagtcag
     2821 cgtgactcga ggaaggtgat caccgccaac acccggcggt gatcgtcgcc ggcctccggc
     2881 aagttcagct ttgtcaatgc tgcgcacctg cttttcgacg gtgccctcgg tcgcccaaag
     2941 ccgatgggta ataccggcat tggaaagacc ctcggctatc aatgccagca cctcacgttc
     3001 ccgcgcgctc agccagccat ggccgaccca gtgcgggacc gtctgtggat acggtgacga
     3061 tttccatcgg ttgagccgac tggtcaccgc cgtgtcgtcg tagccattcc cttcctccgc
     3121 atcccactcg acgatgccag cgccgaactg atgaaggagt ccgaatatgc gtcgaacctg
     3181 ggcgtcggat tcgattgcgc cgcctacgaa cttgttgagt gccaccggaa aatactcgat
     3241 atcgccgctc atagctcacc atccacgact agaaagggct ggctgaccgc agaggtagaa
     3301 ccgcccgcga ccgaaaaggc tggtccaggg cagcgttgac ccagcgcgcg ccgatgccgc
     3361 gcgcaaaaag atgcacgacg ccgtccagtc ccagcgactc gctgatattc gtaaggcgca
     3421 cgggcacgcc cgtcagtctg acgttgccgc gctgatgggg tctcccaggc ccgggtgtcg
     3481 aagctggaga gtggggatct ttcgcacacc gagctcggca ctttgcagtc ctatgttgct
     3541 gcccgcggcg ggaatctaca gatcgtggcc gacttcgggg agagcaccgt ggaactgacg
     3601 gcgtgacgcc gactcgaccc catcgaaggg cccctgctac cgaagacgga caagacgccc
     3661 cagccattgg gtatccgaaa gtctgtggtg ttggtggagc tacggccgtc cgtcatggct
     3721 ggattcggag gggctggtgg cgtttaccac cgcaccgtca accattcccg gatgggggta
     3781 ttacgcgtgg cccgcggcct cttgcgcgag ttgcacccgc gaggtcaggc cgagcttggt
     3841 gtagacgtgg gtgagatggg cttgcacggt gcgcggggag acgaacaacc gtgcggcaac
     3901 ctccttgttg ctcaggcctt cactcacaag tttcacgacg tcgagctctg cgcgggtcag
     3961 cgaagcccag ccgctgctgg cgcgcttgcg ctcacgtcga ccacgcagcg cataggcggt
     4021 tgcctcctcg atcgacaaag ccgcgccctc ggcgcgtgca gcctcgaagt ctttctcgcc
     4081 cagggcatct cgaacggcgg cgatcctggc ctcgtcgcct tcgtcaaaaa tcttgaagcg
     4141 caccaggccc atgtgctgac gagctgcgtc cgctgcaccg aacagccgcg ccgaggacac
     4201 gaagttatcg gtatcaccgg ccactactgc caggcagtcg agtgcgaacg ggaccaccag
     4261 gtcgccccca aggcgggcgg ccaagtcaag tgcgtcgtac gcatcgcgat cggcagcgtc
     4321 gagctcaccc tgtgcgatct ccacacgtga ccgcgacgtc agggccgccg ctaggctcca
     4381 gttcgtggtc gccgacacga catcatccgc ccagcgacgg gcagcggcga gatcgccgga
     4441 tgccagcgga ccaagcggcg cccaggtgta tatccccgag gtcatgggat ccatcacggt
     4501 gcgctcgcgc gcggcttcat atgcttccca cgctgcggac gcatcgcccg tggttaggtg
     4561 aacggtgccc aaggctgcgt gggcaattcc tatgaagtac tccatggact cggatgcgcg
     4621 ttcgaggttc tcaaccgccc gtgcccgcgc gttcgcaaca tct
//
LOCUS       DASYLX010000747         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2563750,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000747 DASYLX010000000
VERSION     DASYLX010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563750"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /gene="cysC"
                     /locus_tag="VGP27_17545"
     CDS             <1..1381
                     /gene="cysC"
                     /locus_tag="VGP27_17545"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV7853135.1"
                     /translation="QVAARLGATDVTVIPIAAKHGDNVVHRSDRTPWYEGPTLLQYLE
                     GVELSAPQPEPGKLRLPVQWVSRPSTEQRRRYTGRLSGGTLQVGDTVVSLPAGTRSTV
                     TVVDTLDDDRTTAVAPLSVAIELADDIDVGRGDVFVSGADDAVLPVAARELDATVCWF
                     IDHPLRAGDRLALKQGARTVRATVQGLHTRLDPETLEELDNPVELVLNDIGSVTLRTS
                     SVVVSDAYTENRDSGAFILIDETTNDTVGAGTITEPREVKPGAQARNDIRWHPSALDR
                     SHRWTATGQRGATIWFTGLPASGKSTIAVAVERALVESGDVAYLLDGDNIRHGLSDDL
                     GFSAGDRAENIRRVGHLTRLFADAGVVALASLVSPLKSDRAIARTLSDAAKLPFIEVY
                     VSTSLAECEKRDPKGLYARARTGELKGLTGVDAPYEAPENPDLVLDTTGAEIDELVAQ
                     VIGLLNQRR"
     gene            complement(1378..>1928)
                     /locus_tag="VGP27_17550"
     CDS             complement(1378..>1928)
                     /locus_tag="VGP27_17550"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401538.1"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEV7853136.1"
                     /translation="GVDAQTGDVRSWEPKPDTDVVVSNATLQWVPEHVELLVRWAGAL
                     DAGSWIAVQMPGSFNTPSHEAVREVARREPFANRLRDMPFPEHDRVSPAAEYAGILTD
                     AGCRVDAWETTYIHELTGEHPVLDWMSGTALRPVIERLSDEAWQQFRQQLIPVLDETY
                     PRRPDGRTFYPFRRIFVVAQVA"
BASE COUNT          323 a          685 c          627 g          293 t
ORIGIN      
        1 ccaggtggcg gcgcgactcg gcgccaccga cgtcaccgtc atccccatcg ccgccaaaca
       61 cggcgacaac gtcgtgcacc ggtcggaccg cacaccctgg tacgaagggc ccacgctgct
      121 gcagtacctc gagggcgtcg agctgtccgc accgcagccc gagccgggca agctgcgact
      181 gccggtgcag tgggtgtcaa gaccatcgac cgagcagcgc aggcgctaca ccggccgcct
      241 gtcggggggc acactgcaag tcggggatac cgtcgtcagc cttcccgcgg gaaccaggtc
      301 gactgtcacc gtcgtcgaca ccctcgacga cgaccgcacg acggccgttg cgccgctgtc
      361 cgttgcgatc gagctcgccg acgacatcga cgtcggccgc ggcgacgtgt tcgtcagcgg
      421 cgccgacgac gcggtgctgc cggtggcggc ccgggaactg gatgccacgg tgtgctggtt
      481 catcgaccat ccgctgaggg cgggtgaccg gctcgcgctc aagcaaggcg caagaaccgt
      541 gcgcgccacc gttcaggggc tgcatactcg gctcgacccg gagaccctcg aggagctcga
      601 caacccggtt gagttggtgc tcaacgacat cggctcggtc acgctgcgca ccagctcggt
      661 cgtcgtctcc gacgcctaca ccgaaaaccg ggacagcggc gcgttcattc tcatcgacga
      721 gacgaccaac gacaccgtcg gcgcaggcac catcaccgag ccacgagaag tcaagccggg
      781 ggcgcaggct cgtaacgaca tccgttggca tccatcggcg ttggaccgct cacaccggtg
      841 gacggccacc gggcagcgcg gcgccacgat atggttcacc ggcctgcctg catcggggaa
      901 gtcgacgatc gcggtggcgg tcgagcgggc gttggtcgag tccggcgacg tcgcctacct
      961 gctcgacggt gacaacatcc gtcacggtct ttccgacgat ctcggcttct ccgcaggcga
     1021 ccgcgccgag aacatccgcc gcgtcgggca tctcacccga ctgttcgccg acgcaggcgt
     1081 ggtcgcgctg gcatcgctgg tgtcgccgct gaagtccgac cgcgcgatcg cccgcaccct
     1141 cagcgacgcc gcgaagctgc cgttcatcga ggtgtacgtg tcgacctcgc tggccgagtg
     1201 tgagaagcgc gaccccaagg gtctctacgc gcgggcgcgc accggagaac tcaaggggct
     1261 caccggcgtc gacgccccgt acgaggcgcc ggagaatccc gatctggtgc tcgacacgac
     1321 cggcgccgag atcgacgagc tggtcgcgca ggttatcggc ctgctaaatc agcgacgcta
     1381 agccacctgc gcgacaacga atatccgccg gaacgggtag aacgtcctgc cgtcgggtcg
     1441 acgtggatag gtctcgtcca gtaccgggat gagctgctgg cggaactgct gccaggcctc
     1501 gtccgacaag cgctcgatca ccggtcgcag tgcggtgccc gacatccaat ccagcactgg
     1561 atgctcgccg gtcaactcgt gtatgtaggt ggtctcccac gcgtccaccc ggcaacccgc
     1621 gtcggtgagt atgccggcat actcggcggc cgggctgacc ctgtcgtgtt cgggaaacgg
     1681 catgtcgcgc agtctgttcg cgaatggttc gcggcgagcc acctcgcgca ccgcctcgtg
     1741 tgacggcgtg ttgaagctgc cgggcatctg caccgcgatc cacgaacccg catccagcgc
     1801 ccccgcccat cgcacgagca actcgacgtg ctcgggcacc cactgcagcg tcgcgttgct
     1861 caccacgacg tcggtgtcgg gcttcggttc ccaggaccgg acatcgccgg tctgcgcgtc
     1921 cacacccc
//
LOCUS       DASYLX010000748         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1466870,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000748 DASYLX010000000
VERSION     DASYLX010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466870"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP27_17555"
     CDS             complement(<1..306)
                     /locus_tag="VGP27_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853137.1"
                     /translation="MGEEIRCLVTGATGYIGGRLVPRLLEAGLTVRAMARNPDKLAKV
                     PWHDEVEVVRGDLTDPQSMTEAFAGVDVVYYLVHSMGTSKDFVAEERQSARNVVEAAQ
                     "
     gene            complement(375..809)
                     /locus_tag="VGP27_17560"
     CDS             complement(375..809)
                     /locus_tag="VGP27_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEV7853138.1"
                     /translation="MTPIRSFALAAVAITAVSVAPPGTAAGTPPRDTEGTVLWQTTDD
                     GKDYVFREITIAPGGSTGWHSHDGQLFGVVKEGTLMHYRADCSMDGIYVADQSIAEKG
                     GPDYVHIGRNLGPGPLVLQVLYIDPAGAPLSDDQPDPGCGFA"
     gene            918..1991
                     /gene="purM"
                     /locus_tag="VGP27_17565"
     CDS             918..1991
                     /gene="purM"
                     /locus_tag="VGP27_17565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV7853139.1"
                     /translation="MTDHAAQHGISYASAGVDIEAGDRAVELFKPWASKATRPEVRGG
                     LGGFAGLFALRGGYREPVLAASTDGVGTKLAVAQAMDKHDTVGLDLVAMVVDDLVVCG
                     AEPLFLQDYIAVGRTVPERVSELVSGIADGCVIAGCALLGGETAEHPGLMAPDHYDIS
                     ATGVGVVEADDVLGPDRVKPGDVIIGMGSTGLHSNGYSLARHVLLEIDHMNLAGHVEE
                     FGRTLGEELLEPTRIYAKDCLALAAETQVRTFCHVTGGGLAGNLERVIPPGLAAEVDR
                     GTWTPAPVFGMIAQRGRVDRVEMEKTFNMGVGMIAVVAPEDTDRALAILTARHLNCWI
                     LGTVKKGSKEGPRAELVGQHPRF"
     gene            complement(2004..2195)
                     /locus_tag="VGP27_17570"
     CDS             complement(2004..2195)
                     /locus_tag="VGP27_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEV7853140.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPQTDFERLRQELAGSEATDTDDR
                     NGAGGLADDEWVPEDDWRR"
     gene            complement(2331..3404)
                     /locus_tag="VGP27_17575"
     CDS             complement(2331..3404)
                     /locus_tag="VGP27_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEV7853141.1"
                     /translation="MSAVPAPESGPDAGAVWHYGDPFGEQRAAIDEAVVVDRSHRAVL
                     ELSGSGRKSWLHSISSQHVSDLPDGAVTQNLSLDGQGRVEDHWIQTELGDATYLDTEP
                     WRGEPLLAFLRKMVFWADVVIEPVDMAVLSLLGPRLADERVLAALGLDSLPAENTATA
                     LPDGGFVRRMSGPGIELDLVVGRAHAADWLDRLTAAGVRRAGVWAYEAHRVVALRPRL
                     GVDTDERTIPHEVGWIGSAVHLDKGCYRGQETVARVHNLGKPPRMMALLHLDGSTDRP
                     SAGDPVLAGGRAVGRLGTVVDHVDLGPVALALLKRGLPADTALTTGGEAEVPAVVDAD
                     SLPTADVTGAGRLAVERLRGGAR"
     gene            3438..>4076
                     /locus_tag="VGP27_17580"
     CDS             3438..>4076
                     /locus_tag="VGP27_17580"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEV7853142.1"
                     /translation="MTSEPGVVVTLDGQLHDPRTPLLHADDLAVVRGDGIFESLLVRD
                     GRPCLLEAHLGRLTQSARLMELPEPDLPRWRAAVGAAVARWTEQGGGEGVLRLIYSRG
                     RESGGEPTAFAMIAAMPGRVEAARRDGVAAVTLARWLPAEGAGDVPWLLAGAKTLSYA
                     VNMAALRHAERQGAGDVIFISSEGRVLEGPRSTVVIAIEEDGRPCLLTPPPWY"
BASE COUNT          619 a         1379 c         1415 g          663 t
ORIGIN      
        1 ttgcgcggct tccaccacat tgcgcgccga ttgcctctcc tcggcgacga agtcttttga
       61 cgtgcccatc gaatgcacca ggtagtagac gacgtcgacg cccgcaaagg cctcggtcat
      121 cgactgggga tcggtcaagt cgccgcggac cacctcgact tcgtcatgcc acggcacctt
      181 cgcgagcttg tcgggattgc gcgccatcgc ccgcaccgtc aggcccgcct ccaggagtcg
      241 cggcaccagc cggccgccga tgtagccggt cgcgcccgtc accaagcagc ggatctcctc
      301 gcccacagaa cctccatcgt cgtgccccat gagttattcg gagccactcg ccgggcggat
      361 gaccatttcg cgccttaggc aaacccgcag cccggatcgg gctggtcgtc ggacagcggc
      421 gccccggccg gatcgatgta gagaacctgc aacaccaggg gccctggccc gaggttgcgg
      481 ccgatgtgca cgtagtcggg gccgcccttc tccgcgatgc tctggtcggc cacgtagatg
      541 ccgtccatcg agcagtcggc ccggtaatgc atcagggtgc cctccttgac gacaccgaac
      601 agctgaccgt cgtgcgagtg ccagccggtg ctcccgccag gggcgatggt gatctcacgg
      661 aacacgtagt ccttgccgtc gtcggtcgtt tgccacagga cggtgccctc ggtgtctcgc
      721 ggcggagtgc cggcggcggt gccgggcggg gcgacggata cggcggtgat tgcgacggcg
      781 gccagcgcaa atgagcgaat cggggtcacg gagggctacg ttgtcatacc gtcgtcgatc
      841 gtgctcgatg tcgaccagga aacgttcagc ggccggcgca taccgtgcag tcgcccagcc
      901 cggtagattt ggtcgcgatg acggatcacg ccgcacaaca cggcatctcc tacgcatcgg
      961 ccggggtgga catcgaagca ggtgaccgcg ccgtcgaact gttcaaaccg tgggcgagca
     1021 aggcgacccg acccgaggtg cgcggcggcc tcggtggctt cgcgggcctg ttcgcgctgc
     1081 gtggcgggta ccgcgagccg gtactcgccg catcgacgga cggcgtaggc accaagctgg
     1141 ccgtggcgca ggccatggac aagcacgaca ccgtcggcct cgacctcgtc gcgatggtcg
     1201 tcgacgacct ggtggtgtgt ggcgccgagc cgctgttcct tcaggactac atcgccgtcg
     1261 gccgcacggt tcccgagcgg gtgagcgaac tggtgtccgg catcgccgac ggctgcgtga
     1321 tcgccgggtg cgctctgctc ggcggggaga ccgccgaaca tcccgggctg atggcgcccg
     1381 accactacga catctctgcg acgggcgtcg gtgtggtgga ggctgacgac gtgctcggtc
     1441 ccgaccgggt caagccgggc gacgtgatca tcggcatggg atccaccggc ctgcactcga
     1501 acggctactc gttggcacgt catgtgctgc tggagatcga ccacatgaac ctggccggac
     1561 acgtcgagga attcggtcgc actctgggcg aggagctact ggagccgacg cgcatctacg
     1621 ccaaggactg cctggcgctg gccgccgaaa cccaggtgcg gacgttctgc cacgtcaccg
     1681 gcggcggatt ggcgggcaat ctggagcggg tgattccgcc cggtctggcg gccgaggtcg
     1741 atcgcgggac gtggacgccg gcaccggtgt tcggcatgat cgcccagcgc ggtcgcgtcg
     1801 accgtgtgga gatggagaag acgttcaaca tgggcgtcgg catgatcgcc gtcgtcgctc
     1861 ccgaggacac cgaccgggcg ctggcgatcc tcaccgcacg ccatttgaac tgctggatcc
     1921 tcggcactgt gaagaagggc agcaaggaag gcccacgagc cgagctcgta ggtcagcacc
     1981 cgcgctttta aggaaaaagg cgcctatcgg cgccagtcgt cctctggtac ccactcgtcg
     2041 tcggccagcc cgcctgcgcc gtttcggtcg tcggtatcgg tcgcttccga cccggccagc
     2101 tcttgccgga gccgttcgaa atcggtctgc ggtgagctgt atttgagctc acgagcaacc
     2161 ttggtctgct ttgccttagc ccggccgcgg cccatggggg gaccccctcg cgcaataacg
     2221 gagcggccca ataagaaggc ggctccgatc tgtgtgtgtc tattgtcctg ccaacacttt
     2281 accgtgcccg tccgcgctgc gctggcaggc ccttctgacc acgtatcggc tcaccgcgct
     2341 ccgccccgca gtcgctcgac ggccagccgc cccgcgccgg tgacatcggc ggtgggcagc
     2401 gagtccgcgt cgaccactgc gggaacctcc gcttcaccgc cggtcgtcaa tgctgtgtcg
     2461 gccggcagcc cccgcttgag tagcgccagc gcgacggggc cgaggtcgac gtggtcgacg
     2521 acggtgccga gtcggccgac cgcgcggccg cccgcgagta ccggatcgcc cgccgacggc
     2581 cgatccgtgg acccgtcgag atgcagcagc gccatcattc ggggcggttt gcccaggtta
     2641 tgaactcgcg cgacggtctc ctgcccgcga tagcagccct tgtccaggtg caccgcgctg
     2701 ccgatccagc ccacctcgtg cgggatggtc cgctcgtcgg tgtcgacccc gaggcgcggc
     2761 cgcagcgcga ccacgcgatg tgcttcgtag gcccagaccc cggcgcgcct gacgcctgcc
     2821 gcggtcagcc ggtccagcca gtcggcggcg tgcgcgcgtc ccacgacgag gtcaagctcg
     2881 atgcccggcc cgctcatccg gcgaacgaat ccgccgtccg gcagggccgt cgccgtgttt
     2941 tcagccggta gcgaatcgag gcccagcgcg gcgagcaccc gctcgtcggc cagccgcggg
     3001 ccgagcagcg acaacacggc catgtcgacc ggctcgatca ccacgtcggc ccagaacacc
     3061 atcttgcgca ggaacgccag caacggctct ccgcgccacg gttcggtgtc caggtaggtg
     3121 gcgtcgccga gctcggtctg tatccagtga tcttcgacac gaccttgtcc gtcgaggctc
     3181 aggttctgcg tgaccgcgcc gtcgggcaga tcgctgacat gctgcgagga aatgctgtgc
     3241 aaccagctct tgcggccgct gcccgacaac tcgagcacag cgcgatgcga gcggtccacg
     3301 accacggcct cgtcaatggc ggcgcgctgc tcaccgaacg ggtcgccgta gtgccagacg
     3361 gcgccggcgt cgggccccga ttcgggggcg ggaactgctg acatggctcc aactgtacgg
     3421 cgctacgctt cttgcccatg acgagcgaac ccggtgttgt ggtgacgctc gacgggcagt
     3481 tgcatgaccc gcgaacaccg ctgctgcacg ccgacgacct ggccgtggtg cggggcgacg
     3541 gcatcttcga gtcgctactc gtgcgtgacg ggcggccgtg tctgctcgag gctcatctgg
     3601 ggcggttgac gcagtcggct cggctgatgg agctgccaga accggatctc ccgcggtggc
     3661 gtgctgcggt gggcgccgcg gtggcccggt ggacggagca gggcggcggc gagggcgtgc
     3721 tgcggctcat ttacagccgg ggccgcgaaa gtggcgggga gccaacggca ttcgcgatga
     3781 tcgccgcgat gcccggcagg gttgaagccg cgcgaagaga cggagtcgcc gccgtgacgt
     3841 tggcccgctg gctgcccgcc gagggggccg gcgacgtgcc ctggctgctg gcgggcgcca
     3901 agacgctgtc gtacgcggtg aacatggcgg cgcttcggca tgccgagcgg cagggcgcgg
     3961 gcgatgtgat cttcatcagt tcggagggcc gggtacttga agggccgcgg tcgacggtgg
     4021 tcatcgcgat cgaggaggac ggacgacctt gtctcctgac cccgccaccg tggtat
//
LOCUS       DASYLX010000749         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1357165,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000749 DASYLX010000000
VERSION     DASYLX010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357165"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..632)
                     /locus_tag="VGP27_17585"
     CDS             complement(318..632)
                     /locus_tag="VGP27_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853143.1"
                     /translation="MTPARCFARWTGDRIGVVEVRAKQYKFGSATIGVGAAVAMAALG
                     VTFGDVSAAESPSQVLSPVAPQATGQTVTTTTPPSVPEVSVATPSVTATTPSGFAPAP
                     GP"
     gene            complement(923..>1763)
                     /locus_tag="VGP27_17590"
     CDS             complement(923..>1763)
                     /locus_tag="VGP27_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7853144.1"
                     /translation="NNYGPPYGSAEENFGRMLRRDFKPYRHELIISTKAGWDMWPGPY
                     GQLGGRAYLLASLDESLDRLGLDYVDIFYSHRIDPKTPLEETIGALDTAVRAGKARYI
                     GISSYSAAKTAEAAAIARRLGTPLVIHQPSYSMLNRWIEGELITELKNVGMGAIAFTA
                     LAQGLLTNRYLDKPATDVARATERPTFDDELVTDEVHARLQGLAEIAKRRGQSLAQLA
                     LAWVLRDPTVASALVGASSVAQLKENLGALDHLEFTPEELSEIDRFASDSGIDLWRES
                     SDL"
BASE COUNT          295 a          569 c          588 g          311 t
ORIGIN      
        1 ctgcgctgcc ctggttggcc ggggaactcg gcactcctgg gacgctcaag ccgcagttat
       61 cgcctcagag gagcggtacg tcagcaacgg caccctcgaa cactcgatga atgagcggcc
      121 gaatgacgcc gcggctttca gcttggtcgc aggctgatca tttcctgatt ggccaacgcc
      181 agtcgcagga attcgcgtgc gccgcgatgc ctctggccgg ggacgaaccg gcacgaccaa
      241 tcaccgctgc cagtagtcgc tagcgtcgcg catggcgccg cgcgaatgcg tatcgcggct
      301 ggggttagtc atcccaccta cggaccgggg gccggcgcga agcccgatgg cgtcgtcgca
      361 gtcacgctcg gcgtcgccac gcttacctcc ggaacggatg gaggagtcgt tgtggtgacc
      421 gtctgcccgg tggcctgcgg tgccaccgga ctcaggactt ggctgggcga ctcggcggcg
      481 ctgacgtcac cgaaggtcac accgagtgct gccatggcga ccgccgcgcc aacgccgatc
      541 gtcgccgacc cgaacttata ttgctttgcg cgcacctcga ccactccaat ccggtcgcct
      601 gtccaccggg cgaagcatcg tgccggagtc acccgcgacc ctctaccacc gcaacgcgat
      661 aacctcgtat aaccggtggg aacagcggtg aaacccgaag cgaacgctca tgctacttcg
      721 agtcctgagc tacgcagcgg cgcgccgccg gctggtctgc agacagtggt ggcagtcgac
      781 cgaaacccgg gccaggtgac ggcacaaccg ccgcggacta agtgcactgc ccacgcaggc
      841 tggtcaaccg ggtgatgggt gggaccttgc atttggcgat gtcgtagcgg ccggagctcg
      901 cagagtgttc gccggcatcc gactacaggt cggagctctc ccgccatagg tcaatgccgg
      961 agtctgaagc gaaacggtcg atctcgctca gctcctcggg cgtgaattcg aggtggtcga
     1021 gcgcgccgag gttttccttc agttgcgcga cgctcgatgc gccgacgagt gcggaggcga
     1081 cggtcgggtc gcggagcacc caggcgagtg ccagttgcgc cagcgactgc ccccgccgct
     1141 tggcgatttc ggcgagtccc tgcagccgcg cgtgcacctc gtcggttacc agctcgtcgt
     1201 cgaaggtcgg gcgctcggta gcgcgggcga cgtcggtagc gggcttgtcc agatagcggt
     1261 ttgtgagcag accctgggcg agcgcggtga aggcgattgc acccatgccg acgttcttga
     1321 gctccgtgat gagttcaccc tcgatccagc ggttcagcat cgagtacgac ggctggtgta
     1381 tcaccagcgg ggtgccgagc cgccgcgcga tcgccgccgc ctctgctgtc ttcgcggccg
     1441 agtacgaaga gatgccgatg tagcgcgcct tgcccgcccg caccgcggta tcgagtgcgc
     1501 cgatggtctc ttccagcggt gtcttcgggt cgatgcggtg cgagtagaag atgtcgacat
     1561 aatcgagccc aagccggtca agcgactcgt cgaggcttgc gagcagatag gcgcgaccac
     1621 ctagctgacc gtaggggcca ggccacatat cccatcccgc cttggtggag atgatgagct
     1681 cgtggcggta cggcttgaaa tcccgtcgca gcattcggcc gaagttttcc tccgcggagc
     1741 cgtagggcgg cccgtagttg ttc
//
LOCUS       DASYLX010000750         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3114420,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000750 DASYLX010000000
VERSION     DASYLX010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114420"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..989)
                     /locus_tag="VGP27_17595"
     CDS             complement(144..989)
                     /locus_tag="VGP27_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7853145.1"
                     /translation="MTNTVGRVAGKVAFITGAARGQGREHAVRLAEEGADIIAVDVCG
                     DIDGVGYPGASEADLDETAALVEKLDRRIVTAKADVRDLDGLRAALQRGVDELGRPDI
                     VVANAGISGAPVPAALIDESAWQTMLDINLTGVMHTTKVALPHMSDGRGGSIILVSSM
                     LGLRGGGYMAGYASAKHAVVGLMNSLANELAPQWIRVNSIHPGNILTPMLDNEWFHRT
                     LRPDLAEPTMDDAAAVIGHFHMLPAPLIEARAVSNAVLFLASDEAKYITGAALPIDAG
                     AVAKF"
     gene            1065..2444
                     /locus_tag="VGP27_17600"
     CDS             1065..2444
                     /locus_tag="VGP27_17600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7853146.1"
                     /translation="MKLMSPTDSLFLIGESREHPMHVAGLQLFRPPDGSGPEFIRELH
                     EAIVENDDFQSTFRKHPGKLLGGIANVAWAFDDDVDIDYHLRRSALPRPGRVRELLEL
                     TSRLHGTLLDRHRPLWEANLVEGLEDGRFAVYTKMHHSLLDGVSAQRLMIRSMTPDPD
                     DREVRVPWALGPKHRSRGSGQSRSALQSITGAVGSVASLAPSALSVARAALLEQQLTL
                     PFRAPKTMFNVPIGGARRVAAQSWPLERIRAIKSAAGVTVNDVVLAMCSGALRAYLIE
                     RDALPDTPLVAMVPVSLRTKDEQDAGGNMVGTILCDLATDTADPVKRLDAINTSMRDN
                     KKVFAELPRNQALALSAFLMSGIALGLVPGFVSSAPPPFNLVISNVPGAREPLYWRGA
                     RLDGNYPLSIALDGQALNITMTNNADNLDFGVVGCRRSVPHLQRLLIHLEDSLADLEK
                     AVGIGIRGR"
     gene            2494..3303
                     /locus_tag="VGP27_17605"
     CDS             2494..3303
                     /locus_tag="VGP27_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016933016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853147.1"
                     /translation="MDVDSVLRRNNVRFAGPQEGPVVMLAHGFGCDQNLWRLVARRLS
                     TAYRVVLFDHVGSGAADPAAWNPQRYSSLAGYVDDILDIVSALDLRDVTFIGHSVAAM
                     MGVLSANRDRERFAKLILVAPSPRYIDDDEGYRGGFSRRDIDELLDSLESNYLGWSRS
                     MAPVIMGAPDRPELSDELTESFCRTDPECARVFARTTFLSDNRTDLQEVAVPTLVIDC
                     AYDAIAPREVGQYVLEQIPACELVTVDTDGHCPQLSAPDATAEAIVAFLQR"
BASE COUNT          580 a         1121 c         1067 g          602 t
ORIGIN      
        1 ggggatggtg cgcgacaccg cgacggaccc gcggccggcg ttcgtcgtcg acgacggcac
       61 ttacatctct gcccgttggc cgggtgacac ccataccttc gccagcgccg tgtcggcgaa
      121 gctcagcgaa aagccatgag aattcagaac tttgccaccg ctcccgcgtc tatcggcagc
      181 gccgctcccg tgatgtattt cgcctcgtcg gaggccagga acagcacagc gttgctgacc
      241 gctcgcgcct cgatcagcgg cgcaggcaac atgtgaaaat gcccgatcac cgccgccgcg
      301 tcgtccatcg tgggctcggc aaggtcggga cgcagggtgc ggtggaacca ttcgttgtcg
      361 agcatcggtg tcagaatgtt gccggggtgt atcgagttca ctctgatcca ctgcggcgca
      421 agctcgttgg cgagggagtt catcaggccg acaacagcat gcttggccga ggcgtacccg
      481 gccatatagc cgccgcctcg cagtcccagc atcgagctca ccaggatgat cgatccgccg
      541 cgaccgtcgg acatatgcgg cagtgccacc ttggtggtgt gcatgacgcc ggtcaaattg
      601 atgtccagca ttgtctgcca cgctgactcg tcgatcagcg cggcggggac gggtgcaccg
      661 ctgatgcctg cattggctac gacgatgtcg ggccggccca actcgtcgac tccccgctgg
      721 agcgcggccc gcaggccatc gaggtccctg acgtctgcct ttgcggtcac gatgcgccgg
      781 tccaacttct cgaccagcgc cgcagtctcg tccagatccg cctcgctcgc ccctggatag
      841 ccgacgccgt cgatgtcgcc acacacatcg acggcgatga tgtcggcgcc ttcctccgcg
      901 agccggacgg cgtgttcgcg gccctggccg cgggcagcac cggtgatgaa tgcgaccttg
      961 cctgccactc tgccaaccgt gttcgtcatc gtcacctttc gcctgcgaag gattagcccc
     1021 tataccttgc acgaaccaca gacaaggtgg gtaccgtcac gtcgatgaag ctgatgtcgc
     1081 cgaccgactc actgttcctc atcggcgaat cccgtgaaca tccgatgcat gttgcaggcc
     1141 tgcagctctt ccggccgccc gacggcagcg gccccgagtt catccgcgag ctgcacgagg
     1201 cgatcgtcga gaacgacgac ttccaatcga cgttcaggaa acatcccggc aagcttctcg
     1261 gtggcatcgc caacgtcgcc tgggcgttcg atgacgacgt cgacatcgac taccacctac
     1321 gcagatcggc gttaccgcgg ccgggccgcg tccgcgagtt gctcgagctc acgtcacggt
     1381 tgcatggcac gctgctcgac cggcaccggc ccctttggga ggcgaatctc gtcgaggggc
     1441 tcgaagacgg acgcttcgcg gtctacacca agatgcacca ctcgcttctc gacggcgtgt
     1501 ctgctcagcg cctgatgatc cgctcgatga cgccggaccc cgacgatcgc gaagtcaggg
     1561 tgccgtgggc gctgggcccg aagcacagaa gcagggggtc gggccagtcg cggtcagcgc
     1621 tgcagtcgat caccggcgcg gtcgggtcgg tcgcatcgct ggccccctct gcgctttcgg
     1681 tggccagggc cgcgctactg gagcagcaac tgaccctgcc gtttcgcgcg ccgaagacga
     1741 tgttcaacgt gccgatcggg ggagcgcgaa gggtagcggc acagtcatgg ccgctggaac
     1801 ggattcgcgc catcaaatcc gcggcgggtg tcaccgtcaa cgacgtcgtg ctggccatgt
     1861 gctcgggagc actgcgggcc tatctcatcg aacgcgacgc gctgccggac acgcccttgg
     1921 tggcgatggt gccggtcagt ctccgcacaa aggacgagca ggatgcgggc ggcaacatgg
     1981 tcggcacgat tctgtgcgac ctggccaccg acaccgccga tcccgtcaag cgtctcgacg
     2041 cgatcaacac ctcgatgcgc gacaacaaga aggtattcgc cgagttgccg cggaaccagg
     2101 cgctcgcgtt gtcggcattc ctgatgtccg ggatcgcact cgggttggtg cccggtttcg
     2161 tgtcgtcggc gccaccgccg ttcaatctcg tcatctccaa cgttcctggt gcgcgcgaac
     2221 cgctgtactg gagaggcgcc cgactggacg gcaattaccc cctgtcgatc gcgcttgacg
     2281 gacaagccct gaacatcacg atgaccaaca acgccgacaa cctcgacttc ggcgtcgtcg
     2341 gctgccgccg cagcgttccg catctgcagc gcctcctcat ccacctggag gattccctgg
     2401 ccgatctgga gaaggccgtc gggatcggca ttcgaggccg ttgactcagg tggccgatac
     2461 cttaggctca gcgaagatga gggaaatagc gtgatggatg tcgacagtgt cctccgcaga
     2521 aacaacgtca gattcgccgg tccgcaggag gggccggtgg tgatgttggc gcacggcttc
     2581 gggtgcgacc agaatctgtg gcggttggtc gcccgacgcc tgtccaccgc gtatcgcgta
     2641 gtgctgttcg accatgtcgg ctcaggagcc gccgaccccg cagcctggaa tccgcagcgg
     2701 tacagctctc tggccggcta cgtcgacgac atcctcgaca tcgtctcggc ccttgaccta
     2761 cgcgacgtca cgttcatcgg gcactcggtg gcggcgatga tgggcgtgct gtcggcgaac
     2821 cgcgaccgcg aacggttcgc caagctgatt ctggtggcgc cctccccccg ctatatcgat
     2881 gacgatgagg gataccgcgg tggattctcc cgtcgtgaca tcgacgagtt gctcgactca
     2941 ctggagagca attacctcgg ctggtcgcgg tcgatggcgc cggtgatcat gggcgcgccc
     3001 gaccgcccgg agctctccga cgagctcacg gagagcttct gccgcaccga tcccgagtgc
     3061 gcgcgggtgt tcgcccgcac gaccttcctg tcggacaacc gcaccgacct acaggaagtg
     3121 gcggtgccga ccttggtgat cgactgtgcc tatgacgcga tcgcgccgcg cgaggtcgga
     3181 cagtacgttc tcgagcagat acccgcttgc gaactggtca cggtcgacac cgacggccac
     3241 tgcccgcagc tgagcgcccc ggatgccacg gcggaggcaa tagtggcatt cctgcaacgg
     3301 tgaacacgaa gtcaccgggg tggcgcgacg acggagacga cctcttcgaa aacgcgccgt
     3361 gcggttatct
//
LOCUS       DASYLX010000751         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2016860,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000751 DASYLX010000000
VERSION     DASYLX010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016860"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..910
                     /locus_tag="VGP27_17610"
     CDS             161..910
                     /locus_tag="VGP27_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853148.1"
                     /translation="MTVPLQRANRPISQSPLEEQISWFDSDTECTMVVSTPAADPALW
                     AEFVDGAARSYFKHGIEQALDIDSLADEANTQLFHVLVNDAGVIMGGIRCKGPLERPE
                     ESHAIVEWAGRPGLRTVRKMIGDRLPFGVVEMKTAWMSDDPCRSRALAGTLARTPFAA
                     MALLDAQFVVATAGSHVLNRWRSSGGVVATKIPAAPYPDDRYRTKMMWWDRSTFTNDA
                     APEQLSKIFDDMAIVARQLDHVGARALGSGL"
     gene            907..3039
                     /locus_tag="VGP27_17615"
     CDS             907..3039
                     /locus_tag="VGP27_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1355c family protein"
                     /protein_id="HEV7853149.1"
                     /translation="MTPAGEESFTATLLIEAQPNDRQLLSELRADSSIEIVDRSDEQL
                     DELRRLRPVPGPDVIAEPPIWAFYPWRRTVTSILGPRGFRAVRLNRNRNLITSEEQER
                     FGRLRIGIVGLSVGHAIAHILAQEGLCGRLRLADFDELELSNLNRVPATVFDVGVNKA
                     KVAARRIAELDPYLPVEVFDRGITTESVDRFLDGVDILVEECDSLDTKVLVRQAARAR
                     RIPVLMATGDRGTLDVERFDVEPERQILHGLLGDVDFAELSGLPSEDKVPYALRMMDG
                     GRLSARMAASLIEVGTTLGTWPQLVAEVAQSSALVAEAVRRIGLGEGLASGRVRTDIA
                     RDLDEIHDPSASTATAQAAGDRRGEVAPTDAIGRVVAAAVRAPSGGNVQPWRISTHNQ
                     AVTIALAPEYTSTMDVRYRGSAVAVGAAAFNARVAAAADGVLGPVSLQPGDDAHPLQA
                     VLRLADGSDRELAALYDAMLARETNRHHGRQVSLTEEVVERLHAVAEREGARLHLLTT
                     PNDVAKAAAILAESDRVRYLTPRLHSEMISEVRFPGDASQDTGIDVRSLELFPADLVK
                     LDVLRRGDVMAYLHKWGLGTALGTDTSERIRTSSCVAVVSTDGQALTDYARAGSAVEA
                     VWVVAQQNGLAVQPVSPVFLYAHDDEDLQTLSSTYAPTLRRMNDEFRRLSAIESGEAL
                     ALVLRFSDAPATSVRSQRRRVDASTLMA"
     gene            3108..>4175
                     /locus_tag="VGP27_17620"
     CDS             3108..>4175
                     /locus_tag="VGP27_17620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7853150.1"
                     /translation="MAVNAATSAAVSQRVLADLVGHFGLDVSFLRYNDHKIHATRLIA
                     EWPVRPDVPDPDPLGLVYFVDADPVFAAAEHAKEILLFRPEPATDDYQKTIEEARNIP
                     GVTMAVVPMLSGDVTTGCLGFVKFGDRAWTPKELNALKAIASLFAQLQARIAAEEQLR
                     FLAEHDDLTGLNNRRALIAHLDHRLAPGQPGPVSALFLDLDRLKAINDYLGHTAGDWF
                     IRVFSRRLREGAEGPNLIARLGGDEFVVVPVVPMSAVEAETLATRLQATLRERVSIDG
                     EMLTRTVSIGVALGVPGRDTTSDLLRRADQALLAAKTAGGNKIAVFSDDMSLKGEFRN
                     DIELHLQSAIENGALVLHYLPE"
BASE COUNT          731 a         1385 c         1355 g          704 t
ORIGIN      
        1 aatctttgcc ggacaagatc ggcccgctag ctggaataac ggtcttgaaa agagcaatac
       61 tcgatgtctg ggtattcata tagaccgcat gtgaatgccg cctcggtaac cgcgactact
      121 cgtccattct ggtcgccgtc acccgaacga tgggacagaa atgaccgttc ctctgcagag
      181 ggccaaccgg ccgatttccc agtcgccgct cgaggagcaa atcagttggt tcgattccga
      241 caccgagtgc acgatggtgg tttcaacacc tgccgccgac cccgcccttt gggccgaatt
      301 cgtcgacggc gcggcgcgca gctacttcaa gcacgggatc gagcaggcgc tggacatcga
      361 ctcgcttgca gatgaggcaa atacccagct gttccatgtc ctagttaacg atgccggagt
      421 gatcatgggc ggaatccgtt gcaaggggcc gctggagagg cctgaggagt cacacgccat
      481 cgtcgagtgg gcgggccggc ccggcctgcg cacggtgcgc aagatgatcg gcgaccgact
      541 gccgttcggc gttgtggaga tgaagaccgc ctggatgtcc gacgacccct gccggagccg
      601 agcactcgca ggcacgctgg cgcgcacccc gttcgccgcc atggcactgc tggacgcgca
      661 gttcgtggtc gcgacagccg gatcacacgt gctcaaccgc tggcgctcgt ccggcggcgt
      721 ggtggcaacg aagattcccg cggcgccgta tcccgacgac cgataccgta cgaagatgat
      781 gtggtgggat cgcagcacct tcaccaacga cgccgcgcct gagcagcttt cgaagatctt
      841 tgacgacatg gccatcgtcg cgcgtcagct tgaccatgtg ggtgcccggg cactcggcag
      901 cggcctgtga ccccagccgg cgaagagtcc ttcaccgcaa cgctgctcat cgaggcacaa
      961 ccgaatgacc gacaactgtt atcggagttg agagcagact ccagcatcga aatcgtcgac
     1021 aggtccgacg aacagctcga tgaattgcgc cgactccgtc cagtgcccgg tcctgacgtc
     1081 atcgccgagc cgccgatttg ggctttttac ccttggcgac gaaccgtcac aagcattctg
     1141 ggtccgcggg gcttccgggc cgtgcggctc aaccgcaacc gaaatctgat cacctcggag
     1201 gaacaggagc ggttcggccg cctccggatc ggcatcgtcg ggctcagcgt cggccatgcc
     1261 atcgcccaca ttctggcgca ggagggactc tgcggtcggc tgcgcctcgc ggacttcgac
     1321 gaactggagt tgtctaacct caaccgggtg ccggccaccg tcttcgacgt gggcgtgaac
     1381 aaggcgaagg tggcggccag acggattgcc gagcttgatc cctatctacc ggttgaggtg
     1441 ttcgaccgcg gaatcacgac ggagtcggtc gaccggttcc tcgacggtgt cgacatcctc
     1501 gtggaggagt gcgactcgct ggacaccaag gtcctcgtcc gccaggcggc ccgcgcgcga
     1561 cgcatcccgg tcctgatggc caccggtgac cgcggcacct tggacgtcga acgcttcgac
     1621 gtggagccgg agcgccaaat tctgcatggc ctgttgggcg acgtggactt cgctgagctc
     1681 tcggggctgc ctagtgagga caaggttccc tacgcccttc gcatgatgga cggtggacgg
     1741 ctgtcggccc gcatggcggc atccctcatc gaggttggaa cgaccctggg cacttggccg
     1801 cagctggtgg ccgaggtcgc ccagagctca gcgctggtgg ccgaggccgt caggcggatc
     1861 ggtctcggtg aggggctggc atcgggcagg gtgcgcaccg acattgcgcg cgacctcgac
     1921 gagatccacg acccttcggc gtcgactgcg acggcccagg ccgccggaga ccggcgcggc
     1981 gaggtcgcac cgaccgacgc catcggtcgg gtggtcgccg cggcagtgcg cgcgccgtcc
     2041 ggcggaaacg tccagccctg gcgcatttcg acgcacaacc aggcggtcac gatcgcgctc
     2101 gcgccggaat acacgtcgac gatggacgtc cgttatcgcg gaagtgcggt cgcggtcggc
     2161 gccgcagcct tcaacgcgcg ggtagcggcg gccgccgacg gagtcctcgg acctgtgagc
     2221 ctccaaccgg gagacgacgc gcatccgctg caggccgtct tgcgtctcgc cgatggatcc
     2281 gaccgcgaac ttgcggcgct gtacgacgcg atgctcgcca gagagaccaa ccgccaccat
     2341 ggcaggcagg tctcgctaac cgaggaagtc gtcgaacgcc tccatgccgt tgcagagcgc
     2401 gaaggcgccc ggctacacct gctgaccacg ccgaacgatg tcgccaaggc ggcggccatc
     2461 ctggccgagt ccgaccgcgt ccggtacctg acaccccggc ttcactccga gatgatctcc
     2521 gaagttcgtt tccccgggga tgcgtcgcag gacaccggaa tcgacgtccg aagcctggag
     2581 cttttccccg ccgacctggt caagctggac gttctgcggc gtggggacgt catggcttac
     2641 ctgcacaaat ggggactggg gactgcgctg ggcaccgaca cctccgaacg cattcggacc
     2701 agttcgtgtg ttgcggtggt gtcgacggac ggtcaggcgc tgacggacta cgcgcgggcg
     2761 gggtcggccg tcgaagccgt ctgggtcgtc gcccagcaga atggcctcgc ggtgcaaccg
     2821 gtgtcaccgg tgttcctcta tgcccacgac gacgaagatc tgcagacact ctcgtcgacg
     2881 tacgcgccga ctctgcggcg gatgaacgac gagttccgcc ggctgtcagc tattgagtcc
     2941 ggcgaagcac tcgcgctggt actcaggttc agcgacgcgc ccgccacgtc ggtgaggagt
     3001 cagcgtcgcc gggttgatgc cagtactctc atggcataac tacggacacc ggagcaaacg
     3061 tggctcgtac cctcgacgaa gtcgtcaccg ccgtcgcgac cgaactgatg gcggtgaatg
     3121 cggccacctc cgccgcggtc agtcagcgcg tgctggccga cctcgtcgga cacttcggcc
     3181 tcgacgtcag cttcctgcgg tacaacgacc acaaaatcca cgcgacgagg ttgatcgccg
     3241 agtggccggt ccgccccgac gttcccgacc cagacccgct cggtcttgtg tacttcgtcg
     3301 acgccgatcc cgtgttcgcc gccgccgagc atgcgaaaga aattctgctc ttccggcccg
     3361 agcccgcgac cgacgactac cagaagacca tcgaggaagc ccgcaacatc cccggggtga
     3421 cgatggcggt cgtgccgatg ctgtccggcg acgtcacgac gggctgcctg gggttcgtca
     3481 agttcggcga cagggcgtgg acaccgaagg agctcaacgc gctcaaggcg atcgcctcgc
     3541 tgttcgcaca gctacaagcc cgcatcgccg ccgaggaaca gctccgcttc ctcgccgagc
     3601 acgacgatct gacgggcctc aacaatcgtc gcgcgctcat cgctcatctg gatcaccggt
     3661 tggcgccggg ccaaccgggc cccgtttcgg cgctgtttct ggacctggac cggctcaagg
     3721 ccatcaacga ctacctgggc catacggcgg gcgactggtt catccgcgtg ttctccaggc
     3781 ggctgcgtga gggcgcggaa ggccccaacc tgatcgctcg cctcggcggt gacgagttcg
     3841 tcgtcgtgcc ggtggtgccg atgagcgccg tcgaagcgga aacgctcgcc acccgcctgc
     3901 aggcgacgct gcgtgagcgg gtgtccatcg acggcgagat gctcacccgc accgtgagca
     3961 tcggcgtcgc gctcggggta cccggtcgcg acactacgtc ggatctcctg cgccgcgcgg
     4021 accaggcgct tctggcggct aagaccgcag gcggtaacaa gatcgcggtg ttctccgacg
     4081 acatgtcgct caagggcgag ttccgcaacg acatcgagct gcatctgcag agtgcgatcg
     4141 agaacggcgc cctggtcctg cactatctgc ccgaa
//
LOCUS       DASYLX010000752         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3773392,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000752 DASYLX010000000
VERSION     DASYLX010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773392"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /gene="tet(V)"
                     /locus_tag="VGP27_17625"
     CDS             complement(<1..1256)
                     /gene="tet(V)"
                     /locus_tag="VGP27_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(V)"
                     /protein_id="HEV7853151.1"
                     /translation="MQKPVVDAGRWRVLAPFRSREYSLLMAAVSLSIFADGMFAVVLA
                     LQVIELDNNPVSLSIVTTCWGAAFVAFVLVGGLTADRIKQRTIIIATESVNVIASSAI
                     AVLGLVGALQVWHLAVVSAAMGAAAAFFFPAYSAILPRILPADQLLAANGVEGVVRPV
                     FQRAVGPAVAGIVVGATFPAMGATTVAGLFAVGLLLLVATRLTTKTTVAEPDQERPHM
                     LRDLRDGFAFMVRTPWLLWTLLFASMFVLVVLGPIEVLLPFIAKDRFTDGARTYGFIL
                     AFFGAGSALGAIAVSSRRLPRRYLSVMMAMWSLGSIPLVIVGVTSSFPMMAVATFVIG
                     VTDGAGMVIWGTLLQRRVPTEMLGRVSSLDFFVSLAFMPVSFAIVGPLSKVVSMETIF
                     LVAGLAPVAFAAVAMRAARMRADEVA"
     gene            complement(1398..>2141)
                     /locus_tag="VGP27_17630"
     CDS             complement(1398..>2141)
                     /locus_tag="VGP27_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7853152.1"
                     /translation="GVLSLTLNRPDSLNSLTATMLNGIADALDRAATDPQVKVVRLAG
                     AGRGFSSGAGISADDQAKKAGGLTVLDAANRAIRSIVALPRPVVAVVQGPAAGVGASL
                     ALACDVVLASDKGYFMLAFTKIGLMPDGGASALVAAAVGRIRAMRMALLAEKIPADEA
                     FRAGLVTAVYPVAELDAAVDKTIATLVRGPVVALAKTKDAINATTLTELDAALEREHS
                     GQAALLRGTDYREGIKAFQERRPASFTDS"
BASE COUNT          417 a          753 c          677 g          294 t
ORIGIN      
        1 ttggccactt cgtccgcacg catccgcgcc gcccgcatgg cgacggctgc aaacgcgacg
       61 ggcgccagac cggccaccag gaagatcgtc tccatcgaga cgaccttgga cagcgggccg
      121 acgatcgcga acgacaccgg catgaacgcc agcgagacga agaaatccag gctcgacacc
      181 cggcccagca tctccgtggg aacccgccgc tgcagcagcg tgccccaaat gaccatgccg
      241 gcaccgtcgg tgacaccgat gacgaatgtc gcgacggcca tcatcgggaa cgacgacgtg
      301 acgccgacga tcaccaacgg aatggagccg aggctccaca tcgccatcat caccgacagg
      361 tagcgcctcg gcaggcgcct cgatgacacc gcaatcgcac caagcgcgct gcccgcaccg
      421 aaaaaggcga ggatgaagcc gtacgtcctt gcgccgtcgg tgaatcggtc cttggcgatg
      481 aacggcagca ggacctcgat cggacccaac accacgagca cgaacatgct ggcgaacagc
      541 agcgtccaca acagccacgg ggtgcgcacc atgaaggcga agccgtcgcg caagtcgcgc
      601 agcatatgcg gccgttcctg atccggctct gcaacagtgg ttttcgtcgt cagccgggtt
      661 gccacaagga gcagtaggcc gacggcgaac aatcccgcca cggtagtcgc gcccatcgcg
      721 gggaacgtcg cgcccaccac gatcccggcg actgcgggac cgacagcgcg ctggaacacc
      781 gggcgcacaa ccccttcgac gccgttggcg gcaagcagtt ggtcggcggg caggatcctc
      841 ggcaggatcg cgctgtacgc gggaaagaag aacgccgccg cggcgcccat cgccgccgag
      901 acgaccgcga gatgccagac ctgcagtgca ccgaccagac caagcaccgc gatggccgac
      961 gacgcgatca cgttgacgga ctcggtggcg atgatgatcg tgcgctgttt gattcggtcc
     1021 gcggtgagcc cgcccaccag cacgaatgcg acgaaggccg cgccccagca ggtcgtcacg
     1081 atcgacaacg acacgggatt gttgtcgagt tcgatcacct gcagggcaag tactactgcg
     1141 aacatgccgt cggcgaagat cgacaacgac accgcggcca tcagcaggct gtactcgcgc
     1201 gaccggaatg gtgcgagcac gcgccatcga ccggcgtcga ccaccggctt ctggatctcg
     1261 aattgtgtgc tcacgccctc gatggtcgcc gaccggccat ggtcgcgtcc accgaatttc
     1321 cgttcgccga aaccgacgaa atggcgttac ctactcgcac aagtacgccc aaacgttggt
     1381 ttgggcgtac ttggtcgcta ggagtcggtg aagctggccg ggcgtcgctc ctggaaggcc
     1441 ttgatgccct cgcggtagtc ggtgccccgc aatagcgcag cctggccgga gtgctcgcgc
     1501 tcgagcgccg cgtcgagttc ggtcagcgtc gtcgcgttga tggcgtcctt ggttttcgcc
     1561 agcgccacca ctggaccgcg caccaacgtc gcgatcgtct tgtcgaccgc ggcgtccagc
     1621 tccgcgacgg gatacacggc ggtcaccaga cccgcccgga acgcctcgtc ggcggggatc
     1681 ttctccgcca acagcgccat ccgcatggcc cggatccggc cgacggccgc cgcgacgaga
     1741 gccgaagcgc cgccgtcggg catcagcccg atcttggtga aggcgagcat gaaatagccc
     1801 ttgtccgagg cgagtacgac atcgcacgcc agcgccagcg aggcgccgac acccgcggcg
     1861 gggccctgca cgaccgcgac caccgggcgc ggcagcgcca cgatcgaacg aatggcacgg
     1921 ttcgccgcat cgagcaccgt caacccgccg gccttcttgg cctgatcgtc ggcgctgatg
     1981 cccgcacccg agctgaagcc acggcccgcg ccggccagcc gaacaacctt gacctgcggg
     2041 tcggtggcgg cccggtccag cgcatcggca atgccattga gcatcgtcgc cgtcagcgaa
     2101 ttgaggctgt ccggccgatt cagcgtcagc gacagcaccc c
//
LOCUS       DASYLX010000753         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3661624,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000753 DASYLX010000000
VERSION     DASYLX010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661624"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VGP27_17635"
     CDS             <1..467
                     /locus_tag="VGP27_17635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7853153.1"
                     /translation="GVIHAGNSSQISDGSAALLFMSAEKAKSLGLKPLARVHTATLAG
                     ADPVIMLTAPIPATQKALKKSGLRLEEIGVFEVNEAFAPVPLAWLKDIGADEKKLNPN
                     GGAIALGHPLGGSGARIMTTMLYHMRDKGIQYGLQTMCEGGGQANATIIELL"
     gene            464..1258
                     /locus_tag="VGP27_17640"
     CDS             464..1258
                     /locus_tag="VGP27_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7853154.1"
                     /translation="MTETVAPAALTERRGNVLVITLNRPEARNAVNSALSTAVGDALQ
                     EAQDDAEVRAVVITGAGESFCAGADLKAISRRENLFHPEHGEWGFAGYVHHYIDKPTI
                     AAVNGTALGGGSELALASDLVVAEERAKFGLPEVKRGLIAAAGGVFRIVDHLPRKVAM
                     ELILTGEPMSSADALKWGLINQVVPDGTAVDAALALAERITVNAPLAVWASKRVAMGV
                     DDGVIAGDEPGWTRTMREIGTLIRSEDAKEGPLAFAEKRQPVWKAK"
     gene            complement(1270..1986)
                     /locus_tag="VGP27_17645"
     CDS             complement(1270..1986)
                     /locus_tag="VGP27_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEV7853155.1"
                     /translation="MKTRTRNAMGAAAATAAIVASVPLSINAYADPTPTPQPAVEIPD
                     PQGNCDPFKAAVPGWKGLNDAPVSKALASIPDISTFNSAVSGGLNPAVNITSVLDNGP
                     YVVFAPTNDAFAALEPGQLDALKADPAALTKLDYYHVFLGLLGPEDVHGQRPTQQGAD
                     IKVEGKNGDIKVNGTTKVICGGIQAQNARIYIIDTVLDPANPPEPLTPTVSSSSSTTT
                     TTTSPLPNAEVVPGAEAPAG"
     gene            complement(2066..3346)
                     /locus_tag="VGP27_17650"
     CDS             complement(2066..3346)
                     /locus_tag="VGP27_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853156.1"
                     /translation="MTLAAADKRVPTSRIHWAWVVAVVSFIAILGAAGFRSVPGVMMT
                     PLHHEFGWSHGVVGLAMSVNMTLFGLTAPFAAALMDRFGVRPVLSVALMLIATGSALS
                     VFMTTSWQLVLWWGVLVGVGTGSISMGFVATVATRWFEARRGLITGVLTAASATGQLI
                     FLPIVAEVTTRHGWRWASLIVAAAAVAVVPLVAVFMRNHPQDRGLTAYGATVAASPSA
                     VPASSFRAAFDGLLLGARVPAFWLLAASFAICGMTTNGLIGTHFIPAANDHGMPTTVA
                     AGLLATIGILDVAGTVFSGWLTDRVDPRLLLVVYYTGRGVSLLLLPSLLSPHAEPSTW
                     VFVIFYGLDWVATVPPTIVLCRNYFGMSTPVVFGWVFASHQLGAAIAAAGAGRLRDLQ
                     GDYDLAFHLAAGLCVVAAALCANVRKAQVSAPTA"
     gene            3430..4386
                     /locus_tag="VGP27_17655"
     CDS             3430..4386
                     /locus_tag="VGP27_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853157.1"
                     /translation="MHRVVVLLLPPVVGFDAAIAPTLFSSATGADGNPLYEVVTCGLT
                     VGPVAATSGFDVLPRSGPEALATADTVVIPGTRYRAARVDGVLSAEVTAALALIRPGT
                     RLVSICTGAFILAAAGLLDGRQATTHWKFADALRGLHPQVLVDENVLFVDDGDVLTSA
                     GLAAGIDLCLHIIRRDHGVQVANAVARYCVVPPWREGGQAQFIDRQVPTPDHYSTAPT
                     RDWALGHLDEELTVQRLARHAKMSARTFNRRFREETGESPGAWIRGRRVDRARELLES
                     RDMPIDEVARLSGLGTGGNLRHHLRRGLGMSPSRYRKVYQGA"
     gene            4541..>4978
                     /locus_tag="VGP27_17660"
     CDS             4541..>4978
                     /locus_tag="VGP27_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV7853158.1"
                     /translation="MKPMRILVTGATGYVGSRLVTELLSQGHDVVTTSRNVERLKDFG
                     WYDDVTALPLDAHDPASAQTALADAGRIDVVYYLVHGIGQPDFREADNAAATNVAEAA
                     KKAGVRRIVYLGGFVPEEGELSEHLTSRAEVAHALSIEGGPEVV"
BASE COUNT          860 a         1648 c         1701 g          769 t
ORIGIN      
        1 acggcgtcat ccatgccggg aactcctcgc agatctccga cggctcggcc gcgctactgt
       61 tcatgtcggc ggagaaggcg aagtcgttgg ggctcaagcc tcttgccagg gtgcacacgg
      121 ccaccctcgc gggcgccgat ccggtaatca tgctgactgc tccgatcccc gcgacgcaga
      181 aggcgctgaa gaagtccggc ctgaggctcg aggagatcgg cgtgttcgag gtcaacgagg
      241 ccttcgcgcc ggtgccgctg gcgtggctga aggatattgg cgccgacgag aagaagctca
      301 acccgaacgg cggtgcgatc gcactcggcc acccgctcgg cggatcgggc gcccgcatca
      361 tgacgaccat gctctaccac atgcgggaca aggggattca gtacggtctg cagaccatgt
      421 gcgagggcgg cggccaggcc aacgccacca tcatcgaact gttgtgacgg aaaccgtggc
      481 tcccgccgct ctcaccgagc ggcggggcaa cgtcctcgtc atcacgctga accggcccga
      541 ggcccgcaac gccgttaaca gcgcgctgag caccgcggtc ggtgacgcac tgcaggaagc
      601 gcaggacgac gccgaggtgc gcgccgtggt catcaccggc gccggtgaat ccttttgcgc
      661 cggagccgat ctcaaggcca tatcgcgacg cgagaacctg ttccaccccg aacacggcga
      721 gtggggtttt gcgggctacg tccaccacta catcgacaag ccgaccatcg cggcggtcaa
      781 cggcaccgcg ctgggcggcg gctccgaact ggccctcgcc agcgatctgg tggtggccga
      841 ggaacgtgcg aagttcgggc tgcccgaggt caagcgcgga ctcatcgccg cggccggtgg
      901 cgtcttccgc atcgtcgacc acttgccgcg caaggttgcg atggagctga tcctcaccgg
      961 tgaaccgatg agttcggccg acgcgctcaa gtggggcctg atcaatcaag tcgtgcccga
     1021 cggcaccgcg gtcgacgccg cgctggcgct ggccgaacgg atcaccgtca acgcgccgct
     1081 ggccgtctgg gcgagcaagc gggtggcgat gggcgtcgac gacggcgtca tcgccggaga
     1141 cgaacccggc tggaccagga ccatgcgcga aatcgggaca ctcattcgat ccgaggacgc
     1201 caaggaaggg cccttggcgt tcgccgagaa gcgccagccc gtctggaaag cgaagtaacg
     1261 cgctagccgc tatccggcgg gcgcctctgc cccgggcacg acctcggcat tcggcagggg
     1321 tgacgtggtc gtggtcgtcg tggacgacga gctactcacg gtcggggtca gcggctcggg
     1381 cggattcgcg ggatccagca ccgtgtcgat gatgtagatg cgggcgtttt gggcctggat
     1441 gccaccgcag atcaccttcg tggtgccgtt gaccttgatg tcaccgttct tgccctcgac
     1501 cttgatgtcg gcgccctgct gggtgggacg ctgcccgtgc acgtcctcgg ggcccagcag
     1561 gccgaggaac acgtggtagt agtccagctt ggtgagcgcg gccggatcgg ccttcaaggc
     1621 gtcgagctgg ccgggttcca gggcggcgaa cgcgtcgttg gtcggcgcga acaccacata
     1681 ggggccgtta tccagcaccg aggtgatgtt gaccgccgga ttcagcccac ccgacaccgc
     1741 ggagttgaag gtgctgatgt cggggatgct ggccagcgcc ttgctgaccg gcgcgtcgtt
     1801 cagccccttc caaccgggca cggccgcctt gaacggatcg cagttgccct gcggatcggg
     1861 gatctccacc gcaggctgcg gcgtcggcgt cgggtcggcg tacgcgttga tcgacagcgg
     1921 taccgatgcc acgatcgcgg ccgttgccgc cgctgcgccc attgcgttgc gggtgcgagt
     1981 cttcacttcg gctcctcagc tcgtcagttg ctaggcatgt taagggcagt gatgagccaa
     2041 tgccaaagtc aagatcagag tgatctcaag ccgtaggcgc gctgacctgc gctttgcgaa
     2101 cattcgcaca gagtgctgct gcaacgacgc agagacctgc ggcgaggtgg aaggcgaggt
     2161 cgtaatcgcc ctgcaggtca cgcaatctgc ctgcgcccgc ggccgcgatc gccgcgccga
     2221 gttggtgcga cgcgaagacc cagccgaaga ccaccggcgt actcatgccg aagtaattgc
     2281 ggcacagcac gatcgtcggc gggacggtcg ccacccagtc cagcccgtag aagatgacga
     2341 atacccacgt gctgggttcg gcatgcggtg agagcagcga cggcagcagc agcagcgaaa
     2401 cgccgcggcc cgtgtagtag acgacaagca gcagtcgcgg atccacccgg tcggtgagcc
     2461 aaccggagaa caccgtgccc gcgacgtcca ggatgccgat cgtcgccaac agtccggccg
     2521 cgacagtcgt cggcatgccg tggtcgttgg cggccgggat gaaatgcgtg ccgatcagcc
     2581 cgttggtcgt catgccgcag atcgcgaagc tggcggccag cagccagaac gcgggcaccc
     2641 tggcgcccag cagcagcccg tcgaacgcgg cgcggaagct cgaggcgggt accgcggagg
     2701 gcgaagcggc tacggtcgcc ccgtaagccg ttagccccct gtcctgcggg tgatttcgca
     2761 tgaacaccgc aaccagcggc accaccgcga cggccgcagc ggcgacgatc aacgaagccc
     2821 agcgccagcc gtgccgggtg gtgacctcgg cgacgatcgg caagaagatc aactggcccg
     2881 tcgcgctggc tgcggtgagc actccggtga tcaggccgcg tctcgcttcg aaccagcgcg
     2941 tcgccaccgt cgccacgaag cccatcgaga tcgacccggt cccgacgccg accagcacgc
     3001 cccaccacag aacgagctgc cagctggtcg tcatgaacac gctcagtgcc gatccggtcg
     3061 cgatgagcat cagcgccacc gacagcacgg ggcgcacacc gaaccggtcc atcagtgcgg
     3121 ccgcgaaggg ggccgtcagg ccgaacagcg tcatgttgac cgacatggcc agcccgacca
     3181 cgccgtgcga ccagccgaac tcgtggtgca gcggagtcat catcacgccg ggaaccgagc
     3241 ggaaacccgc ggctccgagg atggcgatga agctgacgac ggccaccacc cacgcccagt
     3301 ggatccggct ggtgggcacg cgtttgtcgg cggcggcgag cgtcaccaga gcactgtgtc
     3361 gcaacggcgt ggtgcgcgca tagtggcaag aatgacaaca cacgtcaaaa acatgccaaa
     3421 atgtcggtca tgcatcgcgt cgtcgtcctg ctccttccgc ccgtggtcgg tttcgatgcc
     3481 gcgatcgcgc cgacgctgtt ctccagcgcg accggcgccg atggcaaccc tctctacgag
     3541 gtggtcacct gcgggctgac cgtcggcccg gtcgcggcga cgtcgggctt cgacgtgctg
     3601 cccagatccg gaccggaggc gttggccacg gccgacaccg tggtgatccc cggcacgcgg
     3661 tatcgggctg cccgcgtcga tggcgtgctg tccgcggagg tgacggccgc gctggcgttg
     3721 atccggcccg gcacccgtct ggtgtcgatc tgcaccggcg ccttcattct cgcggcggcc
     3781 gggctgctcg acgggcggca ggcgaccacg cactggaagt tcgccgacgc gttgcgcggg
     3841 ttgcatcctc aagtgctcgt cgacgagaac gtgttgttcg tcgatgacgg cgacgtcctg
     3901 acgtcggcgg gtctggcggc cggaatcgac ttgtgcctgc acatcattcg gcgggaccac
     3961 ggcgtacagg tggccaatgc cgtcgccaga tattgcgtgg tgccgccgtg gcgtgaaggc
     4021 gggcaggcgc aattcatcga tcggcaggta ccgacgccgg atcactactc cacggcgccg
     4081 acgcgcgact gggcgctggg tcacctggac gaggagctga cggtgcagcg gctggcccgg
     4141 cacgcgaaga tgagcgcccg cacattcaac cgtcgcttcc gcgaggagac cggcgagtcg
     4201 ccgggtgctt ggattcgcgg cagacgcgtg gaccgggcac gtgaactcct cgagtcgcgg
     4261 gatatgccga tcgacgaggt cgcccggctg tcgggccttg gcaccggcgg caatctgcga
     4321 catcacctac gccgaggctt gggcatgtcc ccgtcgcgct atcgcaaggt ctatcagggc
     4381 gcttgacggg tcattgatcg ggccgccgct gcgccgagat cgacgaaatg gcggcgcgct
     4441 ctcgaccttt ctcgcccaaa tgtgagtttg ggcgacactg gccgcccgcg ctggaccaag
     4501 gctccgattc cgtgcaaccg agtgcgggta agaccttacg gtgaaaccaa tgcgaatcct
     4561 ggtcaccggg gcgaccggct acgtcgggtc ccggctcgtc accgaattgc tttcgcaggg
     4621 ccatgacgtc gtcaccacct cccgaaatgt cgagcggttg aaagatttcg gctggtacga
     4681 cgacgtcacc gccctgccac tcgacgccca cgacccggcg tcggcgcaga cggcattggc
     4741 tgacgccgga cggatcgacg tcgtctatta cctggtgcac ggcatcggcc agcccgactt
     4801 ccgggaagcc gacaacgcgg ccgcgaccaa cgtcgcagag gccgccaaaa aggccggcgt
     4861 gcgccgcatc gtctacctcg gcggcttcgt gcccgaggag ggcgagttgt ccgagcatct
     4921 gaccagccgg gcggaggtcg cgcacgcgct cagcatcgag ggcggcccag aggtggtc
//
LOCUS       DASYLX010000754         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3333832,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000754 DASYLX010000000
VERSION     DASYLX010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333832"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGP27_17665"
     CDS             complement(<1..444)
                     /locus_tag="VGP27_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727412.1"
                     /note="catalyzes the dehydration of
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid
                     to form O-succinylbenzoate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylbenzoate synthase"
                     /protein_id="HEV7853159.1"
                     /translation="MASGIIDPVPSVTEIVDRMHVVALPMRVKFRGITVRELALIDGP
                     AGWGEFGAFPEYQPPEAAAWLAAAVEAAYRPPPPTHRDRIPINATVPAVPAAQVPEVL
                     ARFPGARTAKVKVAEPGQTLADDVARVNAVREHVSTVRVDANGGWS"
     gene            643..1125
                     /locus_tag="VGP27_17670"
     CDS             643..1125
                     /locus_tag="VGP27_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEV7853160.1"
                     /translation="MATRTLASTTLAVTATGVLGGLASRPAQSAWYRALKKPPYQPPR
                     QVFPIVWPILYAEIAAVSASTLDDLDDRGQSGARRAYLAALAANLVLNGSWSWLFFTK
                     RRLGMSAIVAGILTVSSADLTRRAASVRGTRAAPLALYPLWCAFASVLSTHIWKLNRH
                     "
     gene            1125..1748
                     /locus_tag="VGP27_17675"
     CDS             1125..1748
                     /locus_tag="VGP27_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7853161.1"
                     /translation="MKITTLPGQIAESLLSIVGIRIGTEEPHYISAPLTDHVELRKYG
                     PRIAAETTVAADEARARNIGFRRLANYIFGGNHRDQEISMTAPVAQQSGGDDIAMTAP
                     VGQSRTSDNGWTIRFFMPSKWTMDTLPAPDDDNVALVTVPGETVAVLRFSGDRSSPAV
                     TAKTEELAKVLRDKGIESTGEPVAWFYDPPWTLPFRRRNEIAMPVKS"
     gene            complement(1752..>2052)
                     /locus_tag="VGP27_17680"
     CDS             complement(1752..>2052)
                     /locus_tag="VGP27_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853162.1"
                     /translation="LRNISVAATRTAPSGRVLGPEQLLTVEQAIRAQTIDAAWQLFSD
                     DEIGSLEVGKYADLVVLSADPRAVSAEKIADLEVRATYLAGRQVYGKLQSCIESA"
BASE COUNT          348 a          700 c          647 g          357 t
ORIGIN      
        1 gctccaaccg ccgttggcgt cgacccgaac cgtcgacacg tgctcgcgga cggcgttgac
       61 gcgggccacg tcgtcggcga gcgtctggcc cggctcggcg accttcacct tggcggtgcg
      121 ggcgccgggg aaccgcgcca gaacttcggg cacttgcgcg gcggggacgg ccgggaccgt
      181 cgcgttgatc ggaatccggt cgcggtgcgt cggcggcggc ggtctgtatg cggcctcgac
      241 ggccgcggcc agccaggccg ctgcttctgg cggctggtac tcggggaagg caccgaactc
      301 gccccagccc gccggcccgt cgatcagcgc cagctcgcga acggtgatgc cccggaactt
      361 cacccgcatc ggcagcgcga cgacatgcat gcgatcgacg atctccgtta ctgacggcac
      421 agggtcaatg attcctgacg ccatggcccg gtcatttttc ggtaatgact tcggggatta
      481 gaaagcgttg ccatcggata catttaagat acttaactac cgcagaaccc tgaggtgtgc
      541 catgaatgtg tccgttcgct cttccgccgt cgcaggcaat accggcgaca accatccgtt
      601 gggtccgcgc acaccagcgc gtagcggagg gcgtcgatag cgatggcaac tcgtactttg
      661 gcttcgacta ctttggcggt caccgcgacc ggtgtgctgg gcggcctggc cagccgcccg
      721 gcacagtcgg cgtggtaccg ggcgctcaag aagccgccgt atcagcctcc gaggcaggtc
      781 tttccgattg tctggcccat tctgtatgca gagatcgcgg ccgtgtcggc gtcgacactc
      841 gacgacctcg acgaccgcgg ccaatccggc gcgcgacgcg catacctcgc cgcactggcc
      901 gccaacctgg tgctcaacgg cagttggtca tggctgttct tcaccaaacg tcggctcggc
      961 atgtcggcga tcgtcgccgg aattctgacg gtcagcagtg ccgacctgac ccgccgcgct
     1021 gcgtccgttc gcggtacccg tgcagcaccg cttgcgctat acccgctgtg gtgcgcgttc
     1081 gcgagcgttc tgtccaccca tatctggaag ctcaatcgac actgatgaag atcaccacgc
     1141 tgcccggcca gattgcggaa tccctactgt cgatcgtcgg cattcgaatc ggcaccgaag
     1201 agccgcacta catcagcgca ccgctcaccg accatgtcga gttgcgcaag tacggtcccc
     1261 gcatcgcggc cgaaacgacg gtcgccgcag acgaggcccg cgcccgcaac atcgggttcc
     1321 ggcgcctggc caactacatc ttcggcggca accaccgcga tcaagagatc tccatgaccg
     1381 caccggttgc ccagcagtcc ggcggcgacg acatcgcgat gacggctcct gtcggccagt
     1441 cacggacgtc cgacaacggt tggacgatcc gattcttcat gccgtcgaag tggacgatgg
     1501 acacgcttcc ggcgcccgac gacgacaacg tcgcgctggt gaccgtaccg ggagagacgg
     1561 tggcggtact gcggttcagc ggcgaccgca gctctccggc cgtcacggct aagaccgagg
     1621 aactggcgaa ggttctgcgc gacaaaggaa tcgagtcgac gggcgagccc gttgcgtggt
     1681 tctacgatcc tccgtggacg cttccgtttc ggcgtcgcaa cgagatcgcc atgcctgtga
     1741 agagctgacg gtcaggcgga ttcgatacag gactgcagct tcccgtagac ctgtcggccc
     1801 gccaggtacg tcgcacggac ctccagatcg gcgatctttt ccgcggacac tgcgcgcgga
     1861 tctgccgaca gcacaaccaa atccgcgtac ttgccgacct ccagcgagcc gatctcgtcg
     1921 tcgctgaaca gctgccatgc cgcgtcgatc gtctgagccc ggatggcctg ctcgacggtg
     1981 agcagctgct cgggcccgag cacccggcca ctcggcgccg tcctcgtcgc cgcgacgctg
     2041 atgttgcgca gc
//
LOCUS       DASYLX010000755        10344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1467491,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000755 DASYLX010000000
VERSION     DASYLX010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467491"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1475
                     /locus_tag="VGP27_17685"
     CDS             354..1475
                     /locus_tag="VGP27_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853163.1"
                     /translation="MLRESSPVMRVDGIGVLVSTWELVDEVLHKPELYSSALTSGILK
                     NNRPLIPLQVDPPEHGKFRKILDPLFAPKRMKSLETSIAVLVNELIDGFIDRDEIDFA
                     KEFSVPFPTQVFLTMLGLPLEELPDLLAMKDGIIRPHEVVGEPLGHPDTDAYQAATAA
                     SIYAYFTETLQDRTAEPRDDMLSWFLEAEIDGDRLSGDEILDICFLFLIAGLDTVSAS
                     LDCFFSYLAQDPQLRRTIASDPSSIPFVVEELLRWETPVMAVAREARHDTELGGCPIK
                     AGEQVLAMIGSANTDENNSPDAGAVRFDRKVNRHFGFGGGVHRCLGSHLARIELRTAL
                     GLWHERIPDYHIKPGVELDFTPGVRAVQTFPMILHQGGE"
     gene            1516..2214
                     /locus_tag="VGP27_17690"
     CDS             1516..2214
                     /locus_tag="VGP27_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7853164.1"
                     /translation="MEFDRPQCLNAFTVAGYRGLREALDRYATDDTARVVVLTGRGRA
                     FSAGADRSLLNAALPDGQRRQAGEEFGQLLESLGGFPKPLFAAVNGLAVGFGATMLLY
                     CDVVILAETARLRFPFTALSIVPEAGSSVLLPARARWADTMWAMLSSEWIDAAEAVRT
                     GLALRCVPDVELVDEVTNSAAVIAAHDPRAVAATKRLLTRSAAGEVRSAIRRELEEML
                     AIQSAGEGRMTPGS"
     gene            2484..3932
                     /locus_tag="VGP27_17695"
     CDS             2484..3932
                     /locus_tag="VGP27_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7853165.1"
                     /translation="MPALREQILRRKPVEEMAAETGADTGQGELKRTIGLWSLSAIGI
                     GGTIGTGIFFILSQAVPMAGPAVIWSFIIAGAVAGLTAVCYAELAGAVPVAGSSYSYT
                     YATLGEFVAMGVGACLLLEWGVAGAAVAVGWSEYVNQFINNVFGVQIPEALSNAPEQG
                     GIINLPAVVLIMMCALLLIRGVSESAKTNAVMVVIKICVLIMFIVIGVQGWNADNLAD
                     FAPFGFSGITAAAGVIFFTFVGLDSVAAAGEEAKNPRRNLPLAIMIALGTVLVLYVLT
                     ALVAVAAQPSDQFDGQEAGLAQILENVVGAKWPGTVLAAGAIISIFSVTLVSIYGQTR
                     ILFAMSRDGMIPEIFHRVNPRTLTPVPNTIIVAVVIGLMAGLIPINFLAEMTSIGTLV
                     AFICVSIGVIVLRRRSPDLPRTFKVPLYPVTPILSVLGALAIVYTLRPITIYVFLVWI
                     AVAFAWYFFYSRSHSHLARGQHVGLAGESNQP"
     gene            3929..4807
                     /locus_tag="VGP27_17700"
     CDS             3929..4807
                     /locus_tag="VGP27_17700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7853166.1"
                     /translation="MTIVVGYPPNRKGKAVLNLAALLSRSSGEDLVVCIVAPLPWLPG
                     VVREDTAFQKQIDDMTDGALAQARADLPADLPATFTTVKARSTPTGLLDAAQQHDASL
                     IVVGSSAAGLFGRISLSSVADRLLHSSHVPVALAPRGFRSSHTAKVDCVTVAYTGTEQ
                     CDSLLRVAADLVSRLGTKMRLASFAVAPAPPVTAMFRTESAEVVAEWSATIQAAARRV
                     LGDTKVGGADVVIGQGSDWESALNDIDWHDGDLLVVGSSESGPIARVFLGSRAAKIIR
                     HTPVPVLVGPRGAVEK"
     gene            complement(4825..5523)
                     /locus_tag="VGP27_17705"
     CDS             complement(4825..5523)
                     /locus_tag="VGP27_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEV7853167.1"
                     /translation="MTEQRNRELAVRMAELARVSAAPRSVNDVLSDVTTAAKELIPGT
                     ESAGILMVAKGGKFESLAGTDDLPHQLDELQIKFSEGPCMQAALDETIVRTDDFRSED
                     RWPLYSPAVVEIGVLSGLSLKLYTANRTAGALNLFAFRPNAFDPADETIGTVLAAHAA
                     AAILASREGEELQSALSTRDRIGQAKGIIMERFGIDDIRAFEMLRRLSQDSNTRLVDI
                     AARVIDTRGDSAGN"
     gene            5763..6995
                     /locus_tag="VGP27_17710"
     CDS             5763..6995
                     /locus_tag="VGP27_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV7853168.1"
                     /translation="MGGSRNVWILGGYQSDFSRNLAREGLDFADLTSEVVESTLAAAR
                     LNANAIEVVHVADAFGELFARQAHLGAMPATVHEGLWDTPSTRHEAACASGSVATLAA
                     IADLRSGAYRTALVVGIELEKTVPGDTAAEHLGAAAWIGHEGTDAKFVWPNMFARVAD
                     EYDRRYGLDESHLRAIAQLNFANARSNARAQTRGWTVPDLLGGGDADELNPVIEGRLR
                     RFDCSQMTDGGAGVVLVTDDYLRGHPDARPIGLIDGWGHRTVGLGLQQKLDRAAADPY
                     VMPHVRGAVLDAFDRANVTLEDIDGFEVHDCFTPSEYLAIDHIGLTGPGESWKAIENG
                     EIEIGGALPINPSGGLIGGGHPVGASGVRMLLDAAKQVSDDAGGYQVEGAKTFGTLNF
                     GGSTSTTVSFVVGSAKES"
     gene            6995..8593
                     /locus_tag="VGP27_17715"
     CDS             6995..8593
                     /locus_tag="VGP27_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEV7853169.1"
                     /translation="MDVEVVGKYLSTLPEDDDHPYRTGPWRPQTTEWDADDLTAVEGE
                     IPRDLDGVYLRNTENPLHPSLKFYHPFDGDGMIHIVGFRDGKAFYRNRFVRTDGLLAE
                     DQAGGALWPGLAEPVQVAKRDYGWGARTLMKDASSTDVIVHRGTALTSFYQCGDLYRV
                     NPLTAETVGKEDFNGGFPFEWGVSAHPKVDDATGELLFFTYSKQAPYMRYGVVDADND
                     LVHLTDIPLPGPRLPHDMAFTTNYAILNDFPLFWDPKLLEADIHLPGFHPDMPSRFAV
                     IPRRGGPEDIKWFEADPTFVLHFTNAYEDGDEIVLDGFYEGDPAPADSLTGDNDNPPQ
                     AGGPPACEGSAPGEKAFRFLALDRLQTRLRRWRFNLVTGATTEEQLADSITEFGMINA
                     GYAGLPYRYTYAATGKPGWFLFDGLVKHDLRTGCEERFAFDDGVYGSETAMAPRSNVA
                     GRAAPGAGSAAEDDGYLVTLTTDMNDDASYCLVFDAARVADGPVCKLLLPERISSGTH
                     STWADGSSLRRWHEADSAPAAIGL"
     gene            8627..9472
                     /locus_tag="VGP27_17720"
     CDS             8627..9472
                     /locus_tag="VGP27_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV7853170.1"
                     /translation="MLGLLGDEWTLLVVQQSLMGATRYADFQSRLPISHSVLSNRLRS
                     LADAELLQRRIYQTNPPRSEYLTTPRSRSLWPMLVSIWDWERHWVAEHADDLPQMRHT
                     VCGAGFAPQLTCRRCGELVSEKDVVAQWGPSGSWPRSVPTAATRRRSASSQAGLFPQT
                     MSVLGNRWGFALLVAAFVGMSRFTDFQTQLAAPPGSIADRLSTFAANGVMVATDNRYR
                     LTEKGRALFPVLIPALQWAQRWFHAPEGPAVVLTHTACGGPFTAVMTCDQCARPVRGN
                     QVAAV"
     gene            9569..10015
                     /locus_tag="VGP27_17725"
     CDS             9569..10015
                     /locus_tag="VGP27_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7853171.1"
                     /translation="MLRFDPFSDLDAVTRGLLTSQTGSNRTPRFMPMDLCKLDDHYVL
                     TADLPGVDPGSVDVDVDNGTLTISAHRTARSDESVQWLTNERFFGSYRRQLSLGEGID
                     TSAISATYENGVLTVTIPLAERAKPRKIDIEHGAGQKSIPTTVDAE"
     gene            complement(10085..>10344)
                     /locus_tag="VGP27_17730"
     CDS             complement(10085..>10344)
                     /locus_tag="VGP27_17730"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7853172.1"
                     /translation="TETKLLMLGHAFDVLSCVAVEFRTHFFNFASRTAIERLGAKLDG
                     ILRSHQLVPDGTRRDTVVYSILDVEWPTVRTHLQFRLARHG"
BASE COUNT         1811 a         3392 c         3288 g         1853 t
ORIGIN      
        1 gcgcaggaag cgcaggcgac ggtccgctcc tgaggtccag aaaaatgcgc tgggcccttc
       61 tgcagcaggc aagacacgct gtggtgattc aggcaaggtc tctccatcct ttgttgcgcc
      121 cttgcgcaca gggactgaca cagactataa aaataacatt tggaaaatag tctcggctga
      181 ccaccatggg gcgtatccgc gttgcgccgt gaacaattcg cattcgggca atcacgcggt
      241 tcgccggcac aaaggattgc aaaggacgtt ctgtgacaca ggagtatgag acgtcgggcc
      301 tgaacttgtt ggcggcgccg gagttgaccc gacatcctca gcccacatac aagatgctgc
      361 gtgagtcctc accggtgatg cgggtcgatg gcatcggcgt gctggtctcc acgtgggagc
      421 tggtggacga ggtcttgcac aagcccgagc tttactcctc ggcgctgacg tcgggcatcc
      481 tcaagaacaa tcggccgctc atcccgctgc aggtagaccc gccagagcac ggcaagtttc
      541 gaaagatcct cgatcctttg tttgcgccca aacggatgaa atcgcttgag acgtctatcg
      601 ctgtgctggt caacgaactc atcgacggct tcatcgacag ggatgagatc gatttcgcca
      661 aggagttctc cgtcccgttc cccacgcagg tcttcttgac catgctcgga ctgccgctcg
      721 aggagttacc cgatttgctt gccatgaagg acgggatcat ccgaccgcac gaggtggtcg
      781 gcgagccgct cggccacccc gacaccgatg cctatcaggc ggcaaccgcg gcatcgatct
      841 acgcgtactt caccgagacc ctccaggacc gcacggccga gccgcgagat gacatgttga
      901 gctggtttct tgaagccgag atcgacggcg accgccttag cggtgacgag atcctcgaca
      961 tctgcttttt gttcctcatc gccgggctgg acaccgtatc cgcctccctt gactgctttt
     1021 tcagctacct ggcccaggat ccacaacttc gcagaaccat agccagcgac ccgtcgagca
     1081 ttccgttcgt tgtggaggaa ctgttgcggt gggaaactcc ggtcatggct gtggcgcgtg
     1141 aggccagaca cgacaccgaa cttggtggct gccccatcaa ggccggtgaa caagtgctgg
     1201 cgatgatcgg ttcggccaac accgacgaaa acaattcgcc tgatgcggga gccgtacggt
     1261 ttgaccgtaa ggtcaaccgg cactttgggt ttggtggcgg agtgcaccgc tgcctgggtt
     1321 cgcacctggc tcgcatcgaa cttcgcaccg cactggggct gtggcatgag cgcatccctg
     1381 attaccacat caagcccggc gtcgaactgg acttcacccc gggcgtccgc gccgtgcaga
     1441 cattcccgat gatcctgcac cagggaggcg agtagcgcag tgctgcaaga gcggcgtgac
     1501 ggctccacaa tgtggttgga gttcgaccga ccgcaatgcc tcaacgcttt cacagtcgcg
     1561 ggatatcgcg gcttgcgaga agcgctggac cggtacgcca ctgacgacac ggctcgtgtg
     1621 gtcgtactga ccggccgcgg acgcgcgttc tccgccggcg ccgaccggtc gttactcaac
     1681 gcggcattgc ccgacggtca gcgacggcaa gccggtgaag aattcgggca actcctcgaa
     1741 tcgcttggcg ggttccccaa gccgttgttc gccgccgtca acggcctggc cgtcggattc
     1801 ggcgccacga tgctgctcta ctgcgacgtc gtgatccttg ccgaaaccgc cagacttcgg
     1861 tttccgttca ccgcgttgag catcgtgccc gaggcgggca gctcggtcct tctgcccgcg
     1921 cgtgcgcgct gggccgacac gatgtgggcg atgctgtcca gcgagtggat cgatgccgcg
     1981 gaggccgtcc gtaccggcct cgccttgcgc tgcgttccag atgtcgaact ggtcgacgag
     2041 gtgactaatt cggcggcggt gatcgccgcc cacgatccgc gtgcggtggc ggccaccaaa
     2101 cggctgctga cacgcagtgc tgccggcgaa gtgcggagtg caatccgccg tgagctcgag
     2161 gagatgcttg ccattcaatc tgccggggaa ggtcggatga cgccgggctc ctgagattgc
     2221 ggacgacggt tgcggcctta aggctgtcgg cctcaccggc tcgcgggaca gaaccggtga
     2281 gacctgtgtt cgcatgacag ccggctatca cgtgaacctc acactctgct acccactgtc
     2341 gctccgcgga aacggcttgg ccacggtccg tccaacgtgt cggctacctc ggcgtggatg
     2401 cggcccgaac ggccggtgcg tcaaatgcac gaaagtaccg gccactctgg gccatgatcg
     2461 gcagtgagtt caaggaggag cccatgcccg cattgcgtga acagatattg cgtcgtaagc
     2521 cggtggaaga gatggccgcc gaaaccggcg ccgacaccgg acagggtgag ctcaagcgca
     2581 cgatcggcct gtggagcctg tcggctatcg gcatcggcgg cacgatcggc accggcatct
     2641 tcttcatcct cagccaagca gtcccgatgg ccgggcccgc ggtcatctgg tcgttcatca
     2701 tcgccggagc cgtcgcgggt ctgaccgcgg tctgctacgc cgaactggcc ggtgccgtac
     2761 cggtcgccgg ttcgtcgtac tcgtacacct acgccaccct cggagagttc gtggccatgg
     2821 gagtcggcgc ctgcctgctg ctcgaatggg gcgtagccgg tgcagccgtt gccgtcggat
     2881 ggtccgagta cgtcaaccag ttcatcaaca acgtcttcgg tgtccagata ccggaggcgt
     2941 tgtcgaacgc acccgagcag ggcggcatca tcaacctgcc cgcagtggtc ctcatcatga
     3001 tgtgcgcgct gctgctgatc cggggcgtca gcgagtcggc gaagaccaac gccgtcatgg
     3061 tggtcatcaa aatctgcgtc ctgatcatgt tcatcgtcat cggcgtccaa ggatggaacg
     3121 cagacaacct ggccgacttc gccccgttcg gcttctccgg catcaccgcg gcagcgggcg
     3181 tcatcttttt caccttcgtc ggcctggaca gcgtcgccgc cgccggtgag gaggcgaaga
     3241 acccacggcg aaacctgccg ctcgcgatca tgatcgcgct cggcacggtc ctggtgctct
     3301 acgtgttgac ggcgctcgtc gcggtagccg cccagccctc cgatcagttc gacggccagg
     3361 aggccggcct cgcacagatc ctcgagaacg ttgtcggcgc caaatggccg gggacggtcc
     3421 tcgccgccgg agccatcatt tcgatcttca gcgtgacgtt ggtgtccatc tacggacaga
     3481 cccggatcct gttcgcgatg tcacgcgacg gaatgatccc cgagatattc caccgggtga
     3541 acccgcgcac cttgaccccg gtgccgaaca ccatcatcgt tgccgtcgtc atcggtctca
     3601 tggccggcct gattccgatc aacttccttg ccgagatgac cagcatcggg acactcgtgg
     3661 ccttcatctg cgtgtcgatc ggcgtcatcg tgctacgtcg gcgctcgccc gacctgccgc
     3721 gcaccttcaa ggtcccgctc tatccggtga caccaattct gtctgtgctc ggcgcgctgg
     3781 caatcgtcta taccttgcgg cccatcacga tctacgtgtt cttggtctgg atcgccgtcg
     3841 ccttcgcctg gtacttcttc tactcgcgca gtcattctca tctcgccaga ggccagcacg
     3901 tcggcctcgc cggcgagagc aaccaaccgt gacgatcgtc gttggctatc ccccgaatcg
     3961 gaagggcaag gccgtactca acctggcggc gctgctgtcc cggtcgagcg gcgaggacct
     4021 cgtcgtatgc atcgtcgcgc cgctgccctg gctgcccgga gtggtccggg aggacaccgc
     4081 atttcagaag cagatcgatg acatgaccga cggtgcgctg gcccaggccc gcgccgacct
     4141 gcccgccgac ctgcccgcga cattcaccac ggtgaaggct cgctcgacgc caacgggctt
     4201 gctggacgcg gcccagcagc acgacgcgag cctgatcgtg gtcggctcgt cggcggcggg
     4261 actcttcggt cgcatatcgc tgagcagcgt cgccgaccgg ctgctgcaca gttcgcatgt
     4321 tccggttgcg ttggcgccgc gaggttttcg atcgagccac accgcgaagg tcgactgtgt
     4381 gacggtggcc tacacgggca ccgaacaatg cgattcgctg ctaagggtcg ccgcggatct
     4441 ggtcagccga ctcggcacca agatgcgtct ggcatcgttc gccgttgcgc cggcaccgcc
     4501 cgtgacggcc atgttccgaa cggagtccgc cgaggtggtc gccgaatgga gtgcgaccat
     4561 acaggccgcc gcgcggcgtg tgctgggcga cacgaaagtg gggggcgccg acgtggtgat
     4621 cggacaaggc agcgactggg agtcggcgct caacgacatc gactggcacg acggcgatct
     4681 gctggtcgtc gggtcgagtg agtcggggcc gattgcgcgg gtgttcttgg gttctcgggc
     4741 cgccaagatc atccgacaca caccggtgcc cgttctggtt ggtccccggg gcgccgtcga
     4801 gaagtaacat gccgcgccgg cacctcagtt gcctgcactg tctccgcgcg tatcgatgac
     4861 ccgcgcagcg atgtcgacca acctcgtatt gctgtcctgc gagagccgcc gcagcatctc
     4921 gaacgcgcgg atgtcgtcga tcccgaagcg ctccatgatg atgcctttgg cctgaccgat
     4981 gcgatcgcgc gtggacagag ccgattgcag ctcttcgcct tcgcggctgg ccaatatcgc
     5041 cgcagcggcg tgcgcagcca gcacggtgcc gatcgtttcg tctgccgggt cgaaggcgtt
     5101 gggcctgaag gcgaacagat tgagtgcgcc cgcagtgcgg ttggccgtgt acaacttgag
     5161 ggacaagccg ctgagcacgc cgatctcgac gactgccggc gagtagaggg gccagcgatc
     5221 ctcggagcgg aaatcgtcgg tccggacgat cgtctcgtcg agggctgcct gcatgcacgg
     5281 cccctcgctg aacttgatct gcagctcgtc cagctgatgc ggcaggtcgt cggtacccgc
     5341 aagcgattcg aacttgccac cctttgccac catcaagatc ccggcgctct ccgtaccggg
     5401 gatcagctct ttcgccgcgg tcgtgacgtc ggaaagcacg tcgttgacgc tgcgtggtgc
     5461 cgcggagacg cgggccaact cggccatccg cacagcgagc tcgcggtttc gctgttccgt
     5521 catacccgca gtgttcccaa tgaggccgcg ggaaacacca ccgggtcctc gacggatcaa
     5581 atcgcctgat caacgtttgt cttcaagcat gcttggcaat gctgccagtc aggtgtgcgt
     5641 ggcggtcgac caccgcgcac actgaggtct cgtcggttca gaccaagagc cgacggggcc
     5701 gccccccttc ggtgaacccg attccgcgcc agtcactctg ttagttgtag tatcgtccgg
     5761 ccatgggcgg ctcccgtaac gtctggattc tcggcggcta tcaaagcgat ttcagccgca
     5821 acttggcccg tgaaggtctg gatttcgccg acctgacgtc cgaagtggtc gagtccacgc
     5881 tggccgcggc gcggctaaat gccaacgcga tcgaagtggt gcacgtggcc gacgccttcg
     5941 gcgagctgtt cgcacgccag gcccacctcg gcgccatgcc cgccaccgtc cacgaaggcc
     6001 tgtgggacac gccgtcgacc cggcatgagg ccgcctgcgc ctcaggcagc gtcgcgaccc
     6061 tcgccgcgat cgcggatctg cgatccggcg cctatcggac cgcgttggtc gtcggcatcg
     6121 aattggagaa gacggtgccg ggcgacacgg ccgccgagca tctcggcgcc gccgcctgga
     6181 tcggacacga aggtaccgac gccaagttcg tgtggcccaa catgttcgcc cgcgtcgccg
     6241 acgagtacga ccggcggtac ggcctcgacg agagccacct gcgtgcgatc gcccaattga
     6301 acttcgccaa cgcgcgatcg aacgcgagag cgcagacccg cgggtggacc gtgccggacc
     6361 tgctcggcgg gggcgacgcc gacgagctca accccgtcat cgaaggcagg ctgcgccgct
     6421 tcgactgcag ccagatgacc gacggcgggg ccggcgtcgt cctcgtcacc gacgattacc
     6481 tgcgcggcca ccccgatgcg cgcccgatcg gactgatcga cggttggggc caccgcaccg
     6541 tcgggttggg gttgcagcag aagctcgacc gcgccgcagc cgacccgtac gttatgccgc
     6601 atgtgcgcgg tgcggtgctc gacgcattcg accgagccaa cgtgaccctc gaggacatcg
     6661 acggcttcga ggtgcacgac tgcttcaccc cgagcgaata cctggccatc gaccacatcg
     6721 gtctcaccgg tccgggcgaa tcgtggaagg ccatcgagaa cggcgagatc gagatcggtg
     6781 gggcgttgcc gatcaacccc agcggcgggc tcatcggcgg cggccatccc gtcggcgcct
     6841 ccggtgtgcg gatgctgctc gacgcggcca aacaggtcag cgacgacgcg ggcgggtatc
     6901 aggtggaggg cgcaaagaca ttcggcacgt tgaacttcgg cggcagcacc agcaccacgg
     6961 tgagtttcgt cgtcggatcg gcaaaggagt cctgatggat gtcgaggtcg tcggcaagta
     7021 cttgtcgaca ctgcccgaag acgatgacca tccctaccgg acgggaccat ggcgaccgca
     7081 gaccaccgag tgggacgccg acgatctcac cgccgtcgaa ggtgagatcc ctcgcgatct
     7141 cgacggggta tatctgcgca acaccgagaa cccgctgcac ccgtcgctca agttctacca
     7201 cccctttgac ggcgacggca tgatccacat cgtcggattc agagacggaa aggcgttcta
     7261 ccgcaacagg ttcgtccgca ccgacggcct gctggccgag gaccaagccg gtggtgcgct
     7321 gtggccgggt ctggccgagc ccgtgcaggt cgccaagcgc gattacggct ggggtgcgcg
     7381 gacgttgatg aaggacgcct cgagcaccga cgtcatcgtg caccgcggca ccgcgctgac
     7441 cagtttctac cagtgcggcg acctgtaccg cgtcaacccg ctgacagccg aaaccgttgg
     7501 gaaagaagac ttcaatgggg gcttcccgtt cgaatggggg gtttccgcgc acccgaaagt
     7561 cgacgatgca accggcgaac tgctgttctt cacctacagc aagcaggcgc cctacatgcg
     7621 ctacggcgtg gtcgacgccg acaacgacct ggtgcacctc accgatatcc cgttgcccgg
     7681 cccgcggcta ccgcacgaca tggcgttcac cacgaactac gccattctca acgactttcc
     7741 gctgttctgg gatcccaagc tgctcgaggc ggacattcat ctgcccggat tccatccgga
     7801 catgccgtcg cggttcgcgg tcatcccccg ccgcggcggc cccgaggaca tcaagtggtt
     7861 cgaggccgat ccgaccttcg tcctgcactt caccaacgcc tacgaggacg gcgacgaaat
     7921 cgtgctcgac ggcttctacg agggcgaccc ggcgccggcg gacagcctga caggtgacaa
     7981 tgacaatccc ccgcaagcgg gtgggccccc agcttgcgag gggagtgccc ccggggagaa
     8041 ggcttttcgg tttctcgccc ttgaccggtt gcagacccgg ctgcggcgct ggcgattcaa
     8101 cctggtcacc ggtgccacaa ccgaggaaca gctggccgac agcatcaccg agttcggcat
     8161 gatcaacgcg ggctatgcgg gtctgcccta tcgctatacc tacgcggcga cgggcaagcc
     8221 cggctggttc ctgttcgacg ggctggtcaa acacgacctg cgaaccggct gcgaggagcg
     8281 cttcgcgttc gacgacggcg tctacggcag cgagacggcg atggcgccca ggtcgaatgt
     8341 cgccggccga gcggctccgg gcgccggcag cgccgcagag gacgacggct atctggtcac
     8401 cctgaccacc gacatgaacg acgacgcgtc ctactgcctg gtgttcgacg ccgcccgggt
     8461 ggccgacggc ccggtgtgca aactgcttct gccggaacgg atctccagcg gaacgcactc
     8521 cacgtgggcc gacggatcgt cactgcggcg ctggcacgag gccgacagcg cgcccgccgc
     8581 gatcgggctg taggtggagc ggcctgaccc caacgccgtg gcccgcatgc ttgggctgct
     8641 cggtgacgag tggaccctgc tagtggttca acagagcctc atgggcgcga cccgctacgc
     8701 cgatttccag tctcgcctgc cgatctcgca ctcggtgttg tcgaatcgac ttcggtcgct
     8761 cgccgatgct gagttgttgc agcgtcggat ctaccagacg aacccgccgc gttcggaata
     8821 tctgaccacg ccgcgcagcc ggtcgctgtg gccgatgctg gtgtcgatct gggactggga
     8881 acgacactgg gtggccgagc acgccgacga cctgccgcag atgcgccaca ccgtctgcgg
     8941 cgccggcttc gctccacagc tcacctgccg caggtgcggt gaactcgtca gcgagaaaga
     9001 tgttgtcgcg cagtgggggc caagcggatc gtggccgcgg tcggtgccga cggcagcaac
     9061 gcgccggcga tcggcatcca gtcaggccgg cttgttcccg cagacgatga gcgtgctcgg
     9121 caatcggtgg ggcttcgcac tcctggtggc cgccttcgtc ggcatgagca gattcaccga
     9181 cttccagacc cagctcgcgg caccacccgg atccatcgcc gaccggctgt cgaccttcgc
     9241 ggccaacggg gtgatggtgg cgacggacaa ccgctaccgc ctcaccgaga agggccgggc
     9301 cctgttcccc gtgctgattc cggcgttgca gtgggctcag cggtggtttc acgcgccaga
     9361 gggtccggcg gtggtgctca ctcacacggc gtgtggcggg cccttcaccg cggtcatgac
     9421 ctgcgaccaa tgcgcccgac cggtgcgcgg aaaccaggtc gcagcggtct agcactcgca
     9481 catggagagt gctaatatcg ctggcgcaca gtgatttggt cgcccgccag ggtggcgggc
     9541 tctgagtcga cataggaggt gaattgctgt gcttcgtttt gatccgttca gtgaccttga
     9601 cgccgtgacc cgtggcttgc tgaccagcca gaccggatcg aatcgcactc cccggttcat
     9661 gccgatggat ctgtgcaagc tcgacgacca ctatgtgttg accgccgatc tgcccggcgt
     9721 cgatccgggc tcggtcgacg tggacgtcga caacggcacc ttgacgatct cggcgcatcg
     9781 caccgcccgc agcgatgaat ccgtccaatg gctgaccaac gaacgcttct tcggcagcta
     9841 ccgacgccag ctgtcattgg gtgaaggcat cgacaccagc gcgatctcgg ctacctacga
     9901 gaacggcgtt ctgacggtga cgatcccgtt ggccgagcgc gcgaagccac gcaagatcga
     9961 catcgaacac ggcgcaggcc agaagagcat tccgacgaca gtcgacgccg agtaagcacg
    10021 ccttcgagac tgcggacaga tcgcgactcg agcagatttt gcgatctggc cgcagtctcg
    10081 gcgctcagcc gtgccgcgcc aggcgaaatt gcaaatgcgt acgcacggtg ggccattcga
    10141 catcgagaat cgaatacacc acggtgtccc gccgcgttcc gtccggcacc agctgatggc
    10201 tgcggagaat cccgtcgagc ttcgcaccga gccgctcgat cgccgtgcgg ctggcgaagt
    10261 tgaagaagtg cgtgcggaat tcgactgcca cacaactcaa cacatcgaac gcgtggccga
    10321 gcatcagtag cttggtctca gtat
//
LOCUS       DASYLX010000756         6964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_41801, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000756 DASYLX010000000
VERSION     DASYLX010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41801"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VGP27_17735"
     CDS             complement(<1..708)
                     /locus_tag="VGP27_17735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7853173.1"
                     /translation="MTVKQLLAPLRWSVINTFLFPRTFARTGQFGDGREAAAAEYVLA
                     NARKGDIDDVLTNMDEFAYNKSFLISVGDEKGKLLDAAVKRADTRLALELGTYCGYGA
                     LCIARAAPTAKVYSVEFAEVNAEVARRIWAHAGVDDRITCVVGTIGDGGRTLQALADQ
                     GFNAGSLDLLFLDHVKSAYLSDLDSIMGRGWLHPGSIVVADNVRIPGAPKYREYMRQQ
                     QGKQFETIEHNTHGEYQT"
     gene            complement(749..1942)
                     /locus_tag="VGP27_17740"
     CDS             complement(749..1942)
                     /locus_tag="VGP27_17740"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853174.1"
                     /translation="MTAGVGDSFDAVVIGAGPSGLAVARELKHQHGIKTLTVDRAAAP
                     AISWRTRYDNFRLNTTGFLSHLPGQRIPFTAGRWPTKEDMVTYFDSYVRRQDITLRLG
                     CAVNRIDRAPGGWQLDTSSGEIRAPIIVLATGNYRTPSTPPWPGLRGFNGEFLHSGDF
                     SNAWPYRDRNVLVVGAGNSAADIAAQLADNCAGRVWLAVRTPPHLVRRAMGPLPSDLF
                     LELSAWIPASVVDPVIAHTLRLQWGGDLSGYGFQRPPLGLKATVQQKGRIPTMADELI
                     DAVRAGRVQVVAAVAAIESDRVVLIDGTVVAPDVIIAATGFSADLDGMVGHLGVLDRR
                     GNPRGGFSSHIGGGMFAIGYGIPPSGPLRAIRRHATPLAREIANYTATAPSKSVDPLT
                     VSAVD"
     gene            2321..4600
                     /locus_tag="VGP27_17745"
     CDS             2321..4600
                     /locus_tag="VGP27_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853175.1"
                     /translation="MLHRIARLAIAAPRRTVAVAALVTVAAAIFGIPVANSLSPTGFE
                     DPTSESATATHLLAERFGQGDQALLFTVSSPDGFDSSASRAVGTAIVDHLQRSSNVAT
                     VVSAWTVPPAAASQLISKDGRSGLVVAGIIGGERNAPKHADELSEQLVGERDGVSVRS
                     GGPAMVNWQITRQTQTDLLLMESIAIPLSFLVLVWVFGGLLAAVLPVMVAAMAIVGTL
                     AILRLITFSTDVSIFALNLSTAMGLALAIDYTLLIISRFRDELAHGATRHDALEHTMV
                     TAGRTVLFSATTVALSMAALTLFPIHFLKSLAYAGVVTVALAATVAVVVTPAALVLLG
                     DRLNSLDVRWLARRLLRRPAPAPVPVEQRYWYRSTKFVMRHALPIGLAGVTLLVLLGA
                     PFLGVKWGFPDDRVLPTSAPAHQVGDQLRSNFADDPPKAVTVVVPDANGLGPEQISRY
                     AADLSRVPGITSVSAPTGRFVGGMPTGLPSAPTGVAQGSIYLTASSTAPLFSSTSEAQ
                     LDRLHAVAGPGDRDVYITGTAQTNRDTVYAITSRLPTVLGLIAVIALGLLFVLTGSIL
                     LPVKALVLNVLSLTAAFGAMVWIFQDGHLGALGTTATGTLVTNMPVLLFCIAFGLSMD
                     YEVFLVARIREFWLSSGRTPADNDESVALGVAHTGRVITAAALVMSISFAALTAANVS
                     FMRMFGLGLTLAVLVDATLVRTVLVPAFMHLMGRWNWWVPAPLVWLHERLGIGGGEPV
                     RTAPKHRAPPARRHDSEAV"
     gene            4609..5322
                     /locus_tag="VGP27_17750"
     CDS             4609..5322
                     /locus_tag="VGP27_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV7853176.1"
                     /translation="MSRRHVVCLGDSITRGQLSVDYVKMLASRTSGKPFVFTNEGVNG
                     DLAENTLRRLDSVIDLRPDLVTVLIGANDANASMSEKNSVRATRMKKLSGRPTIEGFG
                     ENVAAIIDRLTKEASARVGLLSLPVLGEELGSESVQRSQQYSEVLKQIAETRGVAYLP
                     LNERQVEHLAAGDFTPRTRYRDGLGLLVGGAIQHVVLRRSLDDISRRRGLQLSTDLLH
                     QNTRGATMIADLIEEFVLQ"
     gene            5558..6391
                     /locus_tag="VGP27_17755"
     CDS             5558..6391
                     /locus_tag="VGP27_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7853177.1"
                     /translation="MRGVVRCVFAVTVIAASVVAEPAGVRLAAASRPYQPAIASVLPT
                     PGQKVGVAHPVVVTFTGPVADRHAAERAIGITSSPAMAGKFEWLGNNVVQWKPDRYWP
                     AHTTMALSVGGVSTSFQTGPAVVGVADIAEHTFTVSIDGVAAGPSTELPAPHHRPHWG
                     EQGVFPASMGRPEYATPVGTYTVLGKERSVTMDSSSVGIPVTDPDGYLLTVDYAVRIT
                     SRGLFVHSAPWAVNSLGYDNVSHGCISLSPEDAEWYFNTVDVGDPVIVLDSQHRVLPS
                     S"
     gene            complement(6420..6872)
                     /locus_tag="VGP27_17760"
     CDS             complement(6420..6872)
                     /locus_tag="VGP27_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853178.1"
                     /translation="MVIFDRLNIRVIAVGVGLCGAAVVALSPDAAADPSAAGGCSQGK
                     EVCAPLADMAGVPMALPGPVPAAPAVPVVPALPAAPAAPLGVPAPLAPPVPVIPAVPA
                     GAPVAAGAPVPAASLFGMSGNGGKGEPTGPAPVGAPVPGQPLPAGPQG"
BASE COUNT         1217 a         2266 c         2201 g         1280 t
ORIGIN      
        1 cgtctgatat tcgccgtgag tgttgtgctc gattgtctcg aactgcttgc cctgctgctg
       61 ccgcatgtat tcgcggtact tcggcgcgcc tggaatccgg acattgtcgg cgaccacaat
      121 cgaacccggg tgcagccaac cgcggcccat gatgctgtcc aagtccgaca ggtatgcgct
      181 tttgacgtgg tcgagaaaca gcaggtccag gctcccggcg ttgaacccct ggtcggctag
      241 agcctgcagt gtgcggccac cgtcaccgat tgtgcccacc acacaggtga tccggtcgtc
      301 cacgccggcg tgcgcccaaa tccgtcgcgc gacctcggcg ttgacctcgg cgaactcgac
      361 ggaatagacc ttggccgtcg gcgcggcccg agcgatgcac aaggcgccgt aaccgcaata
      421 tgtgccgagc tcgagagcaa ggcgggtatc ggcgcgcttg acggcggcgt cgaggagttt
      481 gcccttctcg tcgcccacac ttatcaggaa ggacttgttg tatgcgaatt cgtccatgtt
      541 ggtgagaacg tcgtcgatat cacccttgcg cgcattggct aatacgtact cagcggccgc
      601 ggcctcccgg ccgtcaccga attggccggt tctggcgaac gttcgcggga aaagaaacgt
      661 gttgatgacc gaccagcgca ggggtgcgag caactgcttc acagtcatgg ctgccagcct
      721 acgcggaacg acccggatgg acgagacatc agtccacggc tgaaacagtc agcggatcaa
      781 cggacttcga aggcgctgtc gccgtatagt tagcaatttc ccgggccagg ggggtggcgt
      841 gcctgcggat tgcccgcagc ggcccactgg gcgggatgcc gtatccgatg gcgaacatcc
      901 cgccgccgat gtgcgaggag aagccgccgc gcgggttgcc gcgccggtcg agtacgccga
      961 gatgcccgac catgccgtcg agatcggcac tgaagccggt cgcggcgatg atcacgtccg
     1021 gcgcaacaac ggtgccgtcg ataaggacaa cgcggtcgga ttcgatcgcg gcgaccgcag
     1081 ccacgacctg gacgcgtccg gctcgtaccg cgtcgatgag ttcgtctgcc atcgtcggga
     1141 tgcggccttt ctgctggacg gtcgctttca gcccaagcgg cggccgctga aagccgtagc
     1201 ccgaaaggtc gccgccccac tggaggcgaa gggtgtgtgc gatcaccgga tcaaccacgc
     1261 tcgcaggtat ccaggcagat agttcgagga aaagatcgga tggaagcggt cccattgccc
     1321 gccgcacgag gtgcggcggt gtgcgcaccg caagccagac ccggccggcg caattgtcgg
     1381 caagctgggc cgcgatgtcg gcggccgaat ttccggcgcc gacaaccaga acgttgcggt
     1441 cccggtacgg ccacgcatta ctgaagtcgc cagagtggag gaactcgcca ttgaaaccgc
     1501 ggagcccggg ccagggtggg gtgctgggcg ttcggtagtt gcccgtggcg agcacgatta
     1561 tcggtgctcg gatttcgcct gacgatgtgt cgagttgcca accacctggt gcccggtcga
     1621 tgcggttgac cgcacacccc agccggagcg tgatgtcctg ccgccggaca tagctgtcga
     1681 agtaggtgac catgtcctct ttggtcggcc aacggccggc cgtgaagggg atccgttgtc
     1741 ccggaaggtg cgacaggaaa ccggtggtgt tgaggcggaa gttgtcatag cgcgtacgcc
     1801 acgaaatcgc cggcgccgct gcgcgatcga cggtgagcgt tttgatgccg tgctgatgct
     1861 tgagctctcg tgcgacagca agacctgacg gccccgcccc gatgacaacg gcgtcgaaac
     1921 tgtccccaac cccggcggtc atccgccgtc cccgtccatc ctccgccggc ggcggtcatc
     1981 cgacgtcgtg atgcgaatgg cgccgcggca tcggcggcca gcacggctgg acatcatggt
     2041 ggcggaatca tttcccgcaa cgaatctgcg cgcactcagc attccccttc cctgactacc
     2101 ccctcaggga aaaacgatcc cgaaccgttt gtggttcatc ttcgccatcg aacgcggtgg
     2161 gcatgcgcaa cgacctgagc gttcggatgg cgtactgcgt gacagtaaca tcggccaatc
     2221 gcaaatttgc taaattgacg caggaacttg ccgtggaccc gactcttcgg tacctctgac
     2281 ccatccgggg gagccccgaa gtctgctcaa aggacatgac ctgctgcacc gaattgcccg
     2341 acttgccatc gccgcaccac ggcgaaccgt cgccgtggct gcgctggtga cggttgctgc
     2401 tgccatattc ggcatcccgg tggcaaatag cctctccccc accggatttg aggatccgac
     2461 ttccgaatca gcgaccgcga cgcatttgct cgccgagcgg ttcggccaag gagatcaagc
     2521 acttctgttc accgtctcct cgccggacgg tttcgacagc tcagcgtccc gtgcggtagg
     2581 tacagcgatc gtcgaccacc tacagcggtc atcaaatgtt gccaccgtgg tctccgcgtg
     2641 gaccgtaccg cccgccgccg cgtcccagtt gatcagtaag gacggcaggt ccgggttggt
     2701 cgtcgccggc ataatcggcg gcgaaaggaa cgcgccaaaa cacgccgacg agctgtccga
     2761 gcagttagtg ggcgagcgcg acggggtcag tgtacggtcg ggcggccccg cgatggtcaa
     2821 ttggcagata acccggcaga cgcagacaga cctgctcctg atggagtcga tcgcgattcc
     2881 gctcagcttc ctcgtgttgg tctgggtatt cggcgggctg ttggcggcgg tattgccggt
     2941 catggtcgcg gccatggcga tcgtgggcac gctggcgatc ctgcgtctga tcacgttcag
     3001 caccgacgtg tccatctttg cgctgaacct gagcaccgcg atgggccttg cgctggcgat
     3061 cgactacaca ttgctgatca tcagccgctt ccgcgacgag ttggctcacg gcgcgacccg
     3121 ccacgacgcg ttggagcaca ccatggtgac ggccggccgc acggtgttgt tctccgcgac
     3181 aaccgtcgcg ctgtcgatgg ccgccctgac cttgtttccg atccacttcc tgaagtcgtt
     3241 ggcctacgcg ggagttgtca cagtcgcctt ggccgcgact gtggcagtgg tggtgacacc
     3301 ggccgccctg gtattgctgg gcgaccgatt gaactcgctc gacgtgcgct ggctcgcgcg
     3361 gcgcttgctg cgtcgaccgg cgccggcgcc tgttcctgtc gagcagcggt attggtaccg
     3421 ctcaacgaaa ttcgtcatgc gccatgccct ccccatcggg ttggccggtg tcacgctgct
     3481 ggtgctgctc ggcgcaccgt tcctcggcgt caagtggggc ttccccgacg accgcgtgct
     3541 gccgacatcc gcccctgcac accaggtcgg agatcaactt cgcagcaact tcgccgatga
     3601 tcctccgaag gcggtgacgg tggtcgtccc cgacgccaac ggcctcgggc cagaacagat
     3661 aagccgatac gcggccgatc tctcccgcgt gcctggcatc acttcggtgt ccgcgccgac
     3721 gggcaggttc gtcggcggta tgccaacagg cctaccgtca gcgccgaccg gagtagcgca
     3781 gggcagcata tatctcaccg cctcgagcac ggcgccgttg ttctcatcca cttcggaggc
     3841 gcaactcgac cgacttcatg ccgttgcggg ccctggcgac agggacgtct acataacggg
     3901 gacggcacag accaaccggg acaccgttta cgccatcacc tcacggcttc caacagtgct
     3961 ggggctgatc gccgtgatcg cgctcggact gctgttcgtg ctgacgggaa gcatcctgct
     4021 gccggtgaag gctctggtgc tcaacgtttt gtcgcttacc gccgcattcg gtgccatggt
     4081 ttggattttc caagacgggc atcttggcgc tctcgggacg acggctaccg ggactctggt
     4141 caccaatatg cccgttctgc tgttctgcat cgctttcggt ttgtcgatgg actacgaggt
     4201 cttcctcgtg gcccggatcc gggagttctg gttgtcctcg gggcgcactc ccgccgataa
     4261 cgacgagagc gtcgccctgg gcgtcgcgca taccggtcga gtgatcacgg ccgccgcgct
     4321 cgtgatgtcc atctcgttcg cagcactgac cgccgcaaat gtgtcgttca tgcggatgtt
     4381 cggcctcggc ctgacgttgg cagtactggt tgacgccacg ctggtgcgaa cggtcttggt
     4441 accggccttc atgcatttga tgggccggtg gaactggtgg gtgccggcac cgctggtctg
     4501 gctgcacgaa cgcctcggaa ttggcggggg cgagcctgtc cgcacagcgc cgaagcaccg
     4561 cgcgccgccc gcgcgacggc atgacagcga ggcggtgtag tgacctcgat gtcccgacgc
     4621 catgtcgtct gcctcggcga cagcatcacc cgcggccaac tcagcgtcga ctatgtgaag
     4681 atgctggcca gccgaacctc tgggaagccg ttcgtgttca ccaatgaggg cgtcaacggt
     4741 gatctcgccg aaaacacgct gcggcgactc gattcggtga tcgacctgcg gcccgacctg
     4801 gtgacagtgc tcatcggcgc caacgacgcc aacgcgagca tgtcggagaa aaacagcgtc
     4861 agggcgacac gcatgaaaaa gctcagcggg cggcccacga tcgaggggtt cggtgaaaac
     4921 gtcgccgcga tcatcgaccg gctaaccaag gaagcctccg ctcgggtcgg gctgctctcg
     4981 ctgccggttc tcggagagga actcggctcg gagtcggtgc agcggtcgca gcagtacagc
     5041 gaggttctca agcagatcgc tgaaacgcgc ggtgtggcat accttccgct caacgagcgc
     5101 caagtggagc acttggcggc cggtgatttc acccctcgaa cccgctatcg cgatggcctc
     5161 ggtctattgg tcggcggggc gatacagcac gtcgtcctgc ggcgctcact ggacgacatc
     5221 tcccgacggc gcggtctgca gctgtcaacg gacctgctcc accaaaacac caggggcgca
     5281 acgatgattg ctgatctcat cgaggaattc gtcttgcagt gaccctagtt cccgcgtgtt
     5341 tgcccaccgg ccgcgcggac ctcgcacgtt attcgcttgg cagacttgat tcggcgtggg
     5401 ataaccagtg agcgtgcgag aaccaacgat tcgcaccccg ttgccgaaat gtgacccgat
     5461 cttgatcggg ttgccaacgc gcccttgccc atccgtctgt cgcaggactc tacgatccct
     5521 tattgacatc gttgtaattt cgagggatcg gattgccatg cgcggggttg ttcggtgtgt
     5581 gttcgcagtg accgtgatcg ccgcgagtgt ggtcgcggag ccggccggcg ttcgccttgc
     5641 ggcggcgagc cggccctacc agcccgccat cgcatcggtt ctgccgacac caggccaaaa
     5701 ggtaggtgtt gcgcatcccg tggtggtgac gttcaccggg cccgtcgctg accggcatgc
     5761 ggccgagcgt gccatcggca tcacatcgtc acccgccatg gccgggaagt tcgaatggct
     5821 cggcaacaat gttgtgcagt ggaaacccga ccggtactgg ccggctcaca ccacgatggc
     5881 gctttcggtg ggcggtgtgt caaccagctt ccaaaccggc cctgccgttg tcggtgttgc
     5941 cgatatcgcg gagcacacat tcaccgtgag catcgacggc gtcgcagcgg gaccatcgac
     6001 ggaactccct gcgccgcacc atcgacccca ctggggagaa caaggagtct ttccggcgtc
     6061 gatgggcagg cccgaatacg ctacgcctgt cggcacttac accgtcctgg gcaaagaacg
     6121 ctcggtgacg atggattcga gcagtgtcgg catcccagtg accgaccccg acggatatct
     6181 cctcacggtg gattacgcag tgcggatcac cagccgtggc ctcttcgtgc attcggctcc
     6241 gtgggctgtc aactcactcg ggtatgacaa cgtcagccac ggctgcatca gcctgagccc
     6301 cgaggacgcc gagtggtact tcaacacggt cgatgtcggc gacccggtaa tcgtgctgga
     6361 ttcgcagcac cgggtgctgc cgtcttcata atctcgaacg ccgacctcgg cgtgcgccgt
     6421 cagccctgag gacccgcagg taatggctga cccggcacgg gcgcgccgac cggcgcggga
     6481 cccgtcggct cccccttacc tccgttgccc gacatgccga agagagaggc agccggaacg
     6541 ggcgcacccg cagctaccgg cgcacctgcc ggtaccgccg gaatcaccgg caccggtggg
     6601 gccagcggcg ccggtacacc aagcggcgca gcaggcgcgg cggggagcgc cggcactaca
     6661 ggcaccgccg gggcggcagg caccggcccg ggcagagcca tcggaactcc cgccatatca
     6721 gcgagtggcg cacatacctc cttgccctgc gagcatccgc ctgccgccga tgggtcggcc
     6781 gccgcgtcag gactcaaggc caccaccgcg gcaccgcaca accccactcc gacagcaatc
     6841 accctgatgt tcaaacgatc aaagatcacc atcgacgtcg actccattca ctcgggctca
     6901 gacactgcgc catgccatct tttgaacgac cgcgtcaatt gtcatcagcg catgccgacc
     6961 acgc
//
LOCUS       DASYLX010000757        12104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2895128,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000757 DASYLX010000000
VERSION     DASYLX010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895128"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VGP27_17765"
     CDS             complement(<1..1899)
                     /locus_tag="VGP27_17765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV7853179.1"
                     /translation="MTFNTVLVANRGEIAVRVIRTLREMGIRSVAVFSDADANARHVG
                     EADVAVGIGPAPARQSYLNIDAVVEAARRTGAQAVHPGYGFLSENAAFAAALESAGIV
                     FIGPPVNAIQTMGDKIAAKTTVSAFGVPVVPGIARPGLTDDELVSAAGDIGYPVLVKP
                     SAGGGGKGMRVVHEASALQAALTSARREAGAAFGDDTLFLERFVLNPRHIEVQVLADG
                     YGNVVHLGERECSLQRRHQKVIEEAPSPLLDDASRARIGAAACDTARSVDYTGAGTVE
                     FIVSADRPDEFFFMEMNTRLQVEHPVTEMVTGIDLVEQQVRIAAGEKLPLGQDDITMT
                     GHAIEARVYAEDPARGFLPTGGVVLALAEPHGAGVRVDSGLADGTVVGSDYDPMLAKV
                     IAHADDRSAALRALDRALANTAVLGVTTNVEFLRFLLADPDVIAGRLDTGLLDRRTTD
                     FAAAEPGDDELVAAAAYKWLRSWPTPEGNLWEVPSGWRMGQHAATTVRLHAGERTDHV
                     HLTGTPRAAAAVIEEGESHTLAASLDSDRLVVTLDGLRTDYLVAATDGQLWLNRAGHT
                     AVIDEVREAPVRPDDEHSGDAELVSPMPGSVVAVGVENGQIVEAGAVVVAVEAMKMEH
                     ALTAPVDGV"
     gene            complement(1904..3457)
                     /locus_tag="VGP27_17770"
     CDS             complement(1904..3457)
                     /locus_tag="VGP27_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7853180.1"
                     /translation="MATTPQAGREQHLALVAQLRDKMAVAALGGSAKSRERHVGRGKL
                     LPRDRVNGLLDPGSPLLEVSPLAADGMYDDECPGAGMIVGIGRISGRECMIVANDATV
                     KGGTYYPITVKKHLRAQEIALQNRLPCVYLVDSGGAFLPRQDEVFPDREHFGRIFYNQ
                     ATMSARGVPQIAAVLGSCTAGGAYVPAMSDEAVIVRNQGTIFLGGPPLVKAATGEVVT
                     AEELGGGDLHSRTSGVTDHLAHDDRDALRIVRRIVSTLGPRLDPPWEVTATVDAVADQ
                     TELYDVVPVDARVPYDVHEVITRIVDGGEFAEFKAEYGSTVVTGFARIHGHPVGIIAN
                     NGVLFSESAMKAAHFIELCDKRMVPLLFLQNISGFMVGRDYEAGGIAKHGAKMVTAVA
                     CARVPKLTVVIGGSYGAGNYSMCGRAYSPRFLWMWPNARISVMGGEQASSVLATVRGE
                     MSPEQEEEFKAPIREQYERQGNPYYSTARLWDDGVIDPADTRTVLGLALSVVGQAPVE
                     PVSYGVFRM"
     gene            3632..4207
                     /locus_tag="VGP27_17775"
     CDS             3632..4207
                     /locus_tag="VGP27_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853181.1"
                     /translation="MLIAAAERLVAERGFLAVRLEDIGAAAGVSGPAIYRHFPNKEAL
                     LVELLVGISTRLLAGARAVVAYAHDTASSLDGLIDFHLDFALGESDLIRIQDRDLDHL
                     PEPAKRQVRKAQRQYVEIWVDVLLRLDRADSEADARLMAHAVFGLLNSTPHSVRPGAA
                     KSAGQGTGSDSRAVLREMTIAALSSAGRTGA"
     gene            complement(4218..4373)
                     /locus_tag="VGP27_17780"
     CDS             complement(4218..4373)
                     /locus_tag="VGP27_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853182.1"
                     /translation="MIVLGVILLILGILFGISILTYIGVVLIVIGAVFWILGSTGRAV
                     GGRARWY"
     gene            complement(4416..4625)
                     /locus_tag="VGP27_17785"
     CDS             complement(4416..4625)
                     /locus_tag="VGP27_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853183.1"
                     /translation="MRGGGILGVIVLIWLVIGVVAAYQRDYFKTDANNCATAGTIALT
                     VVAGPLNYAGVNPKVTNCNVPQPSQ"
     gene            complement(4718..6595)
                     /locus_tag="VGP27_17790"
     CDS             complement(4718..6595)
                     /locus_tag="VGP27_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853184.1"
                     /translation="MTQTVRTNYGASLSPDATAFAHLVDDGGYPRAVQRFLRGWRASS
                     SRDVELPVEGPVHRVMHSADGHWLACEVAPEGGSRSQIWVVTTDPDDRAARRIDMWLA
                     GSPEGTAELIGWDGTLVAAILTGDDGVGSSCLIDPADGTTTILDRRSGGRLVDAWAGA
                     ALVRVGPRGYRDLIMLRGLTEIALLPYDPGSTTDTGIILDDQHPRRLRSGLEGEMFEL
                     YQPAKTYGPNSTDGYVRALIRSENGADHARLLEVTTTANGVAYQVIAERAGHELDEFV
                     VSDDLSTVALLWNINGASELQILEYADYTLNNPIPLPGMVASELTISAGGSMVAMTVE
                     GPDMPRTVELVDPRTREWERIDREPSSGPLSAAPTLETITARDGLEFTGWLFRPPEGV
                     DSTGAMMFLHGGPEGQGRPGYNEFFPPLLEAGITVFLPNVRGSGGFGRSFMHADDREK
                     RFAAIDDVADAALFLVDRGLASADCIACCGWSYGGYLTQAALAFHPQLFAAGISICGM
                     SDLNTWYRNTEPWIAAAAYPKYGHPVSDRELLDQLSPLLRAHALTAPLLLVHGLNDTN
                     VPPSESHQMYDALRALGRNVEQLLFEDDGHEIDKRENRSVLVKAMCDWLTTAFAHVPD
                     F"
     gene            6697..7500
                     /locus_tag="VGP27_17795"
     CDS             6697..7500
                     /locus_tag="VGP27_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7853185.1"
                     /translation="MTNTPRGDGSDPAQRLGPSGPGYSDPAYAGQSPYGPGYQAPPPP
                     DPTREMPPYSPYGYDPYGTGQYATDPYGTGQYSTGQYGPPYPPGEPPEGTPSPKSPRW
                     LWVLAAVAVLLVVGLVIALVVVNSSKQDTVVAPPPMPEPSMTTTRTPSTTTSQRPLPP
                     IVPVPTPSTTPPSGSATPGATESVIYEVTGTGRAINITYVDTGSLLQTEFNVVLPWRK
                     EVELPSPAQDSASLTIINVGRQVSCTVTVNGVQVQSNTGNGLTICTAAR"
     gene            complement(7497..8816)
                     /locus_tag="VGP27_17800"
     CDS             complement(7497..8816)
                     /locus_tag="VGP27_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853186.1"
                     /translation="MDDPRPPEQTARRSQVLAWALWDVGSTGLNAIVVTFVFSVYLTR
                     TVADDLPSATTPQSWLFRALALSGLVVALLAPVTGIWVDAPWRRRRVLAVLTGLVVLL
                     TSAMSLIRDDYHYLWPGLALLAMAAACSELATVPYNAMLRQLSTPQTSGRVSGFGWAM
                     GYFGSVVLLFVVYLMFMSGDGATRGLVGLPVAEGQNVRAAMLLAAAWFALFALPVFIV
                     VPHPTPPPADRRQAVGFFGAYRRLWSEVVGEWRRDRNIVYYLLASAVFRDGLAGVFAF
                     GAVLGVSVYGISEADVLLFGVSASVIAAIGALAGGLLDDRIGSKPVIVGSLFSMIIAG
                     LVLMTVSGSVAFWICGLLLCLFIGPTQSSARTLMLRMSVDGKEGVAFGLYTTTGRAVS
                     FLAPTLISLFIDWFGAVRAGVAGLLVVIAFGMAAMVAVHAPHRAVAR"
     gene            8842..9648
                     /gene="cmrA"
                     /locus_tag="VGP27_17805"
     CDS             8842..9648
                     /gene="cmrA"
                     /locus_tag="VGP27_17805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856195.1"
                     /note="Catalyzes the final step in mycolic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycolate reductase"
                     /protein_id="HEV7853187.1"
                     /translation="MPVPAPSPDARAVVTGASQNIGEALAIELAARGHHLIITARRED
                     VLRTLADRITQKYGVVVEVRPVDLADPSARTKLAEELAGRNISILCANAGTATFGPVS
                     KLDPAEEKAQVQLNVLGVHDLVLAVLPGMVQRGAGGILISGSTAGNSPIPNNATYAAT
                     KAFVNTFSESLRGELKRAGVNVTVLAPGPVRETLPAEHEQSLVEKIIPDFLWISTEYT
                     AKLSLDALERNKMRVVPGVTSKAMSVASGYAPRAIVTPIVGAVYKKLGGD"
     gene            complement(9677..11275)
                     /locus_tag="VGP27_17810"
                     /pseudo
     CDS             complement(9677..11275)
                     /locus_tag="VGP27_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
     gene            11355..12002
                     /gene="orn"
                     /locus_tag="VGP27_17815"
     CDS             11355..12002
                     /gene="orn"
                     /locus_tag="VGP27_17815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEV7853188.1"
                     /translation="MRDELVWIDCEMTGLDLKSDRLIEIAVVVTDADLNILGEGIDVV
                     INAPDESLNGMIEVVAQMHDRSGLTEEVRASTIDVPAAEQMVLDYIRSHVKHAKTAPL
                     AGNSIATDRGFIARDMPALDDYLHYRMIDVSSIKELCRRWYPRIYFGQPEKGLAHRAL
                     ADIHESIRELQYYRRTAFVSPPGPSTSDIAAISAELGHEGSNGDGMDSAAERTSG"
     gene            12030..12102
                     /locus_tag="VGP27_17820"
     tRNA            12030..12102
                     /locus_tag="VGP27_17820"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12063..12065,aa:His,seq:gtg)
BASE COUNT         2131 a         4025 c         3963 g         1985 t
ORIGIN      
        1 cacaccgtcg accggagcgg tcagcgcgtg ctccatcttc atcgcctcga ccgcgacgac
       61 gaccgcgccc gcctcgacga tctggccgtt ctcgacgccg acggcgacga cggatcccgg
      121 catggggctg acgagttcgg cgtcgccgct gtgttcgtcg tcgggacgca cgggcgcttc
      181 ccgtacctcg tcgatgaccg cggtgtgccc cgcgcggttc aaccagagtt gcccgtctgt
      241 tgcggcgacg agatagtcgg tgcgcaggcc gtcaagtgtc accacgagcc ggtcactgtc
      301 gagactagct gccagagtgt gtgattcacc ctcttctatg accgccgccg cggcgcgcgg
      361 tgtgccggtc aggtgcacgt ggtcggtgcg ctcgcccgca tgcagccgga cggtggtggc
      421 agcgtgttgc cccatccgcc agcccgacgg cacctcccac aggttgccct ccggtgtcgg
      481 ccagctgcgc aaccacttgt atgccgcggc cgcgacgagc tcatcgtcac ccggttcggc
      541 ggccgcgaaa tcggttgtcc tgcggtccaa cagtccggtg tcgagtcgtc ccgcaatgac
      601 atccggatcg gcaagcagga agcgcaggaa ctcgacgttc gtcgtcacgc ctaacacggc
      661 ggtgttggcc agtgcgcggt cgagggcgcg cagcgccgcg gatcggtcgt cggcatgcgc
      721 gatgaccttg gccagcatcg ggtcgtagtc gctgccgacc acggtgccgt cggcgagccc
      781 ggagtcgacg cgaacgccgg caccgtgggg ctccgccagc gcgagcacca cgccgccggt
      841 cggcaggaac ccgcgcgccg ggtcctccgc atagacgcgg gcttcgatgg catgcccggt
      901 catagtgatg tcgtcctgcc cgagcggcag cttctccccc gcggcgatcc gcacctgttg
      961 ctccacgagg tcgatgccgg tgaccatttc ggtgaccgga tgctcgacct ggaggcgggt
     1021 gttcatctcc atgaagaaga actcgtcggg ccgatcggct gacacgatga actcgacggt
     1081 gcccgcgccg gtgtagtcga cactgcgcgc ggtgtcgcag gctgcggcgc cgatgcgggc
     1141 ccgcgacgca tcgtccagga gcggggacgg cgcctcctcg attaccttct gatggcgacg
     1201 ctgcaggctg cattcacgtt cgccgagatg gaccacgttg ccgtagccgt cggccagcac
     1261 ctgcacctcg atgtggcggg ggttcaacac aaatcgctcg aggaacagcg tgtcgtcacc
     1321 gaatgccgcc cctgcctcgc gccgtgcgct ggtcagtgcg gcctgcagcg cggacgcttc
     1381 gtggaccacc cgcatgccct tgccgccgcc accggccgac ggcttcacca gcacggggta
     1441 gccgatgtcg cctgcggccg aaaccagttc gtcatcggtg aggccgggcc gggcaatgcc
     1501 ggggacgacc ggcacaccga acgccgacac cgtcgtcttg gcggcgatct tgtcgcccat
     1561 ggtctgaatg gcgttcaccg gcggcccgat gaatacgatc cccgccgatt ccagcgcggc
     1621 agcgaaagcc gcgttctccg agaggaaccc gtagcccggg tgaacggctt gagcgccggt
     1681 gcggcgggcg gcttcgacca ccgcgtcgat attgagatag ctctgccgcg cgggagcggg
     1741 accgatgccg accgcgacgt ccgcctcgcc gacgtggcgg gcgttggcat cggcgtcgct
     1801 gaacacggcg accgagcgga ttcccatttc gcgcagcgtg cggatcacgc ggacggcgat
     1861 ctcgccgcgg ttggctacca gaacagtgtt gaacgtcatc acatcacatc cggaacacgc
     1921 cgtaggagac cggttcgacg ggcgcctgcc cgaccactga aagtgccagc ccgagaacgg
     1981 tcctggtatc ggcgggatcg atcaccccgt cgtcccagag ccgtgccgtc gagtaatagg
     2041 ggttgccctg cctctcgtac tgctcccgaa tcggcgcctt gaactcttcc tcctgctccg
     2101 gactcatctc gccgcgcacg gtggccagca ccgacgatgc ctgctcaccg cccatcaccg
     2161 agatcctggc gttcggccac atccacagga agcggggcga atacgcgcgc ccgcacatcg
     2221 agtagttgcc ggcgccgtat gaaccaccga tcacgacggt cagcttcggc acccgggcac
     2281 acgcgacggc ggtcaccatt ttggcgccgt gcttggcgat gccgcctgcc tcgtaatcgc
     2341 ggccgaccat gaagccggag atgttctgca ggaacagcag cggaaccatc cgcttgtcgc
     2401 acagctcgat gaaatgggcg gccttcatgg ctgattcgga gaacagcacg ccgttgttgg
     2461 cgatgatccc gaccggatgg ccgtggatcc gagcgaatcc ggtgaccacc gtcgaaccgt
     2521 attcggcctt gaactctgcg aattcgccgc cgtcgacgat tcgtgtgatg acctcgtgca
     2581 cgtcgtaggg cacgcgcgcg tcgaccggta cgacgtcgta gagctcggtc tggtcggcca
     2641 ccgcatcgac cgtcgcggtg acctcccatg gcgggtcaag gcgtggcccc agcgtcgaga
     2701 cgatacgccg cacgatcctc aaggcgtcgc ggtcgtcgtg agccaggtgg tcggtgacac
     2761 ccgaggtcct cgaatgcagg tcgccaccgc cgagttcttc ggcggtcacc acttcgccgg
     2821 tcgcggcctt caccagcggc gggccgccga ggaaaatggt gccctgattg cggacgatga
     2881 ccgcttcgtc gctcatcgcg ggaacgtatg cgcctccggc ggtgcacgag ccgaggacgg
     2941 cggcgatctg gggaacacct ctggcgctca tcgtcgcctg gttgtagaag atgcggccga
     3001 agtgttcgcg gtcggggaat acctcgtcct ggcggggcag gaacgcgccg cccgaatcaa
     3061 ccaggtagac gcacggcaac cggttctgca gcgcgatctc ctgggcccgc aggtgcttct
     3121 tgaccgtaat cgggtagtac gtgccacctt tgacggtcgc gtcattcgcc acgatcatgc
     3181 actcgcgccc cgatatccgc ccgatgccga cgatcatgcc cgcgccggga cactcgtcgt
     3241 cgtacatgcc gtcagcggca agcggtgaca cttcgagcag tgggctcccg gggtcgagca
     3301 gcccgttaac gcggtccctc ggcagcagct tgccccggcc gacgtggcgc tcgcgcgact
     3361 tggccgagcc gccgagagcg gcaacggcca tcttgtcgcg cagctgagcg acgagggcca
     3421 ggtgctgctc gcggccggcc tgcggcgttg ttgccatcgg tactgccgtt ccggttgagt
     3481 taatgacgac taactcgtag gatgttagtc acgattaact gaaatgtcca ccagcggaaa
     3541 cgggggtaat gcagtgtctc tcccggcgtc gcggtccaat ccgccgcagc ctggcatcag
     3601 ccgcagcaag gcgaaatccg accgtcgcga catgctgatc gccgcggcgg aacggctggt
     3661 cgccgagcgt ggcttcctcg ccgtgcgact ggaggacatc ggtgccgcgg caggtgtcag
     3721 cgggccagcc atctaccggc acttccccaa caaggaagcg ctgctggtcg aactgttggt
     3781 gggcatcagc acccgcctac tcgccggagc cagggctgtc gtggcctacg cgcacgacac
     3841 cgcatcttcg ctggacggat tgatcgactt ccacctcgat ttcgcgctgg gcgagtctga
     3901 tctgatccgc atccaggacc gcgatctcga ccacttgccc gagcccgcca agcgtcaggt
     3961 ccgcaaggcc caacggcagt acgtggagat ctgggtcgac gttcttcttc gactggaccg
     4021 cgcagacagc gaagccgacg cgcgcctcat ggcccacgcc gtcttcggtc tgctgaactc
     4081 gacgcctcac agcgttcggc ccggcgcagc caaatcggcc ggccaaggaa caggttctga
     4141 ctcgcgagcg gtcttgcggg agatgacgat cgcggccctg tcctcggccg gccgaaccgg
     4201 cgcctaggcg ttagttacta gtaccagcgc gctcggccgc cgacagcacg accggtcgag
     4261 ccgagaatcc agaacactgc gccgatcacg atcagcacga cgccgatgta ggtcaggatc
     4321 gagataccga aaaggatccc gaggatgagg aggatcactc caaggacgat catcgttcac
     4381 tccgttcgtt caatgaattg acttgagccc gttgactact ggctaggttg cggcacgttg
     4441 caatttgtca ccttcgggtt cacccccgcg tagttcaacg gcccggcaac gaccgtcaac
     4501 gcgatggtgc ccgctgtggc acagttgttt gcgtccgtct tgaagtaatc gcgctggtag
     4561 gccgcgacaa caccgatcac taaccagatc aacacaatta cgccgagaat tccgccacca
     4621 cgcatgctgg cctcctcctt ttgaacgccg acttggcgca ggagggtttt accccgcgga
     4681 taattccagt aaaccgctaa aactgatctt gagtggttca gaagtcgggt acgtgcgcga
     4741 aggccgtggt gagccagtcg cacatcgcct tgaccagcac ggatcggttc tcgcgcttgt
     4801 cgatctcatg gccgtcgtcc tcgaacaaca attgttcgac gttgcggccc agcgcccgca
     4861 aagcgtcgta catctggtgc gactcgctcg gcggcacgtt ggtgtcgttg agtccgtgga
     4921 cgagaagcag gggcgcggtc aacgcgtgcg cgcggagcaa cggagacagc tgatcgagca
     4981 gttcccggtc gctcaccggg tggccgtatt tggggtaagc cgcggccgcg atccacggct
     5041 cggtgttgcg gtaccaggtg ttcaggtcgc tcattccgca gatgctgatg cccgctgcga
     5101 acaactgagg gtggaacgcc agtgccgcct gtgtcaggta cccgccgtag gaccagccgc
     5161 aacacgcgat gcagtcggcg gacgccagcc cgcggtcgac cagaaacagg gccgcgtcgg
     5221 cgacgtcgtc gatggccgcg aagcgcttct cccggtcgtc ggcatgcatg aacgaccggc
     5281 cgaatccccc ggaaccgcgc acatttggca gaaagaccgt gatacccgcc tccagcagcg
     5341 gagggaagaa ttcgttgtaa ccaggcctgc cctgaccctc tgggccgcca tgcaggaaca
     5401 tcatcgcccc ggtcgagtcg acgccttcgg gtggccgaaa gagccagccg gtgaactcca
     5461 agccgtcgcg tgccgtgatc gtctccagcg tcggagccgc ggacagcggc ccgctgctgg
     5521 gctcgcggtc gatgcgctcc cattccctgg tacgcgggtc gacgagttcc acggtgcgcg
     5581 gcatatccgg gccctcgacc gtcatcgcca ccatcgaacc gcccgcgctg atggtcagct
     5641 cggaggcgac catgcccggc aacgggatgg ggttgttcag tgtgtagtcg gcgtattcga
     5701 ggatctgtaa ctcactggcg ccgttgatgt tccacagtag tgcgaccgtc gacagatcgt
     5761 cgctgacgac gaactcgtcg agttcgtggc ctgcacgctc ggcgatcacc tgataggcca
     5821 cgccgttcgc ggtcgtcgtc acctcgagca gccgggcgtg gtccgctccg ttctcgctgc
     5881 ggatgagcgc acgcacataa ccgtccgtgc tgttcgggcc gtaggtcttc gcgggctgat
     5941 acagctcgaa catctcccct tccagacccg accgaagccg tcgcgggtgc tggtcgtcga
     6001 ggatgatgcc ggtgtctgtg gtcgagccgg gatcgtatgg caacaaagcg atttccgtaa
     6061 ggccacgcag catgatcagg tcgcggtagc cgcgtggtcc gacgcgcacc agggccgcgc
     6121 cggcccacgc gtcgacgagc cggccgcccg accgccgatc aaggatcgtc gtggtgccgt
     6181 cagcgggatc gatcaggcaa gaactgccga cgccgtcgtc gccggtgaga atcgccgcga
     6241 ctagtgtgcc gtcccaaccg atcagctccg cggtgccctc cggcgagccc gcgagccaca
     6301 tgtcgatccg tcgtgcagca cggtcgtcgg ggtcggtggt gacgacccag atctggctgc
     6361 gactgccgcc ctcgggcgcg acctcgcacg ccaaccaatg gccgtcggcg gaatgcatta
     6421 cccggtggac aggaccctca acgggcaatt cgacatctcg tgacgagctc gcgcgccacc
     6481 cgcgaaggaa ccgctgaacc gcccgtggat acccgccgtc gtcgacgagg tgcgcgaacg
     6541 ccgtcgcgtc cggcgacagc gacgcgccgt agttggtgcg caccgtctgg gtcatcaata
     6601 tcagcttgcc acgagcctcc ggcctaagcg atgtgcttac ctgctgtccg cgggtcacct
     6661 ggcgcaacgc aggtaccctg cagccatgag gtgggtatga caaatacgcc acgaggcgac
     6721 gggtccgatc cagcgcagcg gttgggtccc agcggtcccg gatattccga cccggcgtat
     6781 gcgggtcaat cgccttacgg acccggctac caggcaccac cgccgcccga ccccacccgc
     6841 gaaatgccgc cctactcccc ctacggctat gacccgtatg gcacgggtca atacgccacc
     6901 gacccgtatg gcaccggtca atacagcacg ggtcagtacg gtccgccgta cccgcccggt
     6961 gaaccgccgg aaggtacgcc gtcgccgaag tcgccgcgct ggttgtgggt gctcgccgca
     7021 gtggcggtgc tcctggtcgt cggtctggtg atcgcgctcg tggtggtcaa cagctcgaag
     7081 caggacacgg tcgtcgcgcc gccaccgatg ccggagccct cgatgaccac cacacgtact
     7141 ccgtcgacga cgacatctca gcgtccgctg cccccgatcg ttccggtgcc gaccccgagc
     7201 accacgccgc ccagcgggtc ggcgaccccc ggcgcgaccg agtcggtgat ctacgaggtg
     7261 accggaaccg gcagggcgat caacatcacc tatgtcgaca caggcagcct gctccagacc
     7321 gagttcaacg tcgtgctgcc gtggcgcaag gaagtggaac tgcccagtcc cgcacaggat
     7381 tcggcgagcc tgacgatcat caatgtgggc cgccaggtgt cctgcacggt gacggtcaac
     7441 ggagtgcagg tgcagtccaa caccggcaac ggcctgacga tctgcaccgc ggcgcgctag
     7501 cgcgcgacag cccggtgcgg tgcgtgtacc gccaccatgg ccgccatgcc gaatgcgatg
     7561 acgaccagca gtccggcgac gcccgcgcgg acagcgccga accagtcgat gaacagcgag
     7621 atgagtgtcg gcgcaaggaa cgacaccgcg cgcccggtcg tcgtgtagag gccgaacgcg
     7681 acgccctcct ttccgtcgac tgacatgcgc agcatcagcg tccgcgccga cgattgcgtc
     7741 ggcccgatga acagacacag cagaagcccg cagatccaga aagcgacgga tccggagacc
     7801 gtcatcaaga ccagaccggc gatgatcatg gagaacagcg agccgacgat gacgggcttg
     7861 gaccctatgc gatcgtcgag aaggccgccg gccagtgcac cgatggcggc gataacgctg
     7921 gcgctgacgc cgaaaagcag cacgtcggcc tccgagatgc cgtagacgct gacgccgagt
     7981 accgcgccga atgcgaagac gccagccaaa ccgtcgcgga acacggcgct cgcgagcagg
     8041 taatacacga tgttgcggtc gcggcgccac tcgccaacga cctccgacca cagcctgcga
     8101 taggcaccga agaagccgac cgcctgccgt cgatctgcgg gcggcggagt cgggtgcggc
     8161 accacgatga acacgggcag tgcgaacagc gcgaaccacg ccgccgccaa gagcatcgcg
     8221 gcccggacgt tctgaccctc ggcgacgggt aggccgacaa gcccgcgggt ggcgccgtca
     8281 ccggacatga acatgagata gacgacgaaa agcaacacga cgctgccgaa ataacccata
     8341 gcccagccga atccggagac ccggccggac gtctgcggcg tggagagctg ccgcagcatc
     8401 gcgttgtacg gcacggtggc cagctcactg catgcggccg ccattgcgag cagggccagg
     8461 ccgggccaca gatagtggta gtcgtcgcgg atcaggctca tcgccgacgt cagcagcacc
     8521 acgaggccgg tgagcacggc cagcacccgg cgtcgccgcc acggcgcgtc gacccatatg
     8581 ccggtcaccg gcgcgaggag cgccaccacg agtcccgaca gcgccaacgc acggaacagc
     8641 caactctgcg gcgtggtggc actgggcagg tcgtcggcga cggttcgggt cagataaacc
     8701 gagaacacga acgtcacgac gatggcgttc aggccggtcg agccgacgtc ccaaagcgcc
     8761 caggccagta cctgcgatcg ccgagcggtt tgctcaggcg gccgcggatc atccatgatc
     8821 gcccacgata tagtccggcc catgccagta cccgcaccca gcccggatgc ccgcgcagtt
     8881 gtcacaggtg cctcgcagaa catcggcgag gcgttggcga tcgagctcgc cgcgcgaggg
     8941 catcacctga tcatcacggc caggcgcgag gacgtgctca ggacgctggc cgatcggatc
     9001 acgcagaagt acggagtcgt cgtcgaggtg cgcccggtcg acctcgccga tccaagcgcg
     9061 cggacgaagc tggccgagga gctggccggc cgcaacatct cgatcctgtg cgccaacgcc
     9121 ggcacggcga cgttcgggcc ggtgtccaag ctggatccgg ccgaggaaaa ggctcaagtc
     9181 cagctgaatg ttcttggcgt gcacgacctc gtcctcgcag tgctgccggg gatggtccag
     9241 cgcggggcgg gcggaatcct gatctccggt tcgacagccg gcaattcgcc gatcccgaac
     9301 aacgcgacct atgcggccac caaggcgttc gtcaacacgt tcagcgagtc gctgcgcggt
     9361 gagctgaagc gcgcgggcgt gaatgtgacg gtgctggcgc cgggcccggt gcgcgaaacc
     9421 ctgcccgccg aacacgagca gtcgttggtg gagaagataa ttcccgattt cctgtggatc
     9481 tcgacagagt acaccgcgaa gctgtcgctg gatgcgttgg agcgcaacaa gatgcgggtg
     9541 gtgccaggtg tgacatcgaa ggcgatgtcg gtggccagcg gctacgcacc ccgcgcgatc
     9601 gtgaccccga ttgtcggcgc ggtctacaag aaactgggcg gcgactagtc gtttctcgcc
     9661 gaagatggtg cttgggtcag cggcgacggc ggccggtgcc gaaaatgctg cgggtgatct
     9721 cgcggccgat cacagtgccc gccgaccgca ttgcgctctt gaaggccgga ctgctcatca
     9781 tcttgtcgaa cattccgggt tccaccgact tcttcgcggt gaccgtgcct tcggcactcg
     9841 cctcaccgcc cgtttgcggc ggcacgtaga ccggcggcgg cagcgacggg tcatcggcgg
     9901 gcgcttgctc cggcggggca agtcgcgcgt tgagtcgctc gtaggccgac tcgcggtcga
     9961 tcgtctggcc gtactcggcc tgcaacgggc tggcctgtgc ggcggccttg atggcgtccg
    10021 gcccgatggt gtccatcagc gagcgcgggg cacgcatcct ggtccacgcc accggcgtcg
    10081 gtgcccccct ttccgacagc accgtgacga tcgcctcgcc gatacccagc gatgtcagcg
    10141 ccgactccag gtcgtacaca gtggttttcg ggtaggtgcg aactgttttg gacaatgcct
    10201 tctggtcgtc tggggtgaat gcgcggaggg cgtgctggat ccgcgcgccg agctggctga
    10261 gcacattgtt ggggacatcg gtgggcagct gagtgcagaa aaacacgccg acgcccttcg
    10321 accggatcag cttgacggtc tgctcgacct gttcgaggaa cgccttggat gcgtcggtga
    10381 acagcagatg tgcctcgtcg aagaagaaga ccagcctcgg cttgtcgacg tcgccgatct
    10441 cgggcagcgt cgtgaacagg tcggcgagca cccacatcag aaacgtcgag aacatcaccg
    10501 gcctggcggc ctgatcgccc agctcgagca ggctgatgat gccgcgaccc tgggggtcga
    10561 gacgcatcaa atccttcggc tccagctcgg gctcgccgaa gaaggtgtcg gcgccttcgg
    10621 cctcgagatt gaccagcgcg cgcaggatca cgcccgcggt ggtcggcgat accgcgccga
    10681 ggttcttgag ctcgggcttg ccctcatcgc tggtgaggaa ctggatcacc gcacgcagat
    10741 ccttgaggtc cagcagcgac agacccttct gatcggccca gtgaaagatc agaccgagag
    10801 tcgattcctg ggtagcgttg agccccagca cctttgacaa cagaatgggt ccgaaactcg
    10861 agatggtggc acgcaccggc acgccgcttc ccgatgtgcc gagcgacagg aactcgacgg
    10921 ggaaggcggt cggcgtccag tcgtcaccag tgtccgtggc gcgctgctgc gtcttgtcgt
    10981 tcggttcccc ggggcgcgaa aggccggaca ggtcgccctt gacgtcggcc atcatcacgg
    11041 gaacgccggc ggccgaaagc tgctcggcga gcacctgcag cgacttcgtc ttaccggtgc
    11101 cggtcgcacc cgcgacgagg ccgtgacggt tgacggtggc cagcggaatg cgcacctgcg
    11161 cagtcggatc tacggcgccg tcaacgacga cagagcccag ttcgagcgcc tgaccatcga
    11221 cggcttaacc ggcggcgatc tgctgggcgg gggttgctgt cgactcggtg gtcatgaccg
    11281 aaccctactt tgagaacacc gtcgcggtgg gtgtgctggg gcatatacca ggtgtgggcc
    11341 tactgttaat cgctgtgcgt gacgaactgg tatggatcga ctgtgagatg accgggttgg
    11401 acctgaaatc cgaccggctc atcgagattg cggtcgtggt gaccgatgcc gacctcaaca
    11461 tcctcggcga gggcatcgac gtcgtcatca acgcccccga cgaatcgctg aacggaatga
    11521 tcgaagtggt cgcccagatg cacgaccggt ccgggctgac cgaggaggtg cgggcatcga
    11581 ccatcgacgt gcctgccgcc gagcagatgg tgctggacta catccgcagc cacgtcaagc
    11641 acgccaagac cgctcccctg gcgggcaact cgatcgccac cgaccgcggc ttcatcgccc
    11701 gcgacatgcc agccctggac gactacctgc actaccggat gatcgacgtc agctccatca
    11761 aggagctgtg caggcgctgg tatccgcgga tctacttcgg ccagccggag aagggtctgg
    11821 cgcaccgtgc gttggccgac atccacgagt cgattcgcga gctgcagtac taccggcgta
    11881 cggcgttcgt atcaccgccg gggccgtcga ccagcgatat cgcggcgatc tccgctgaac
    11941 tgggccacga aggctccaac ggggacggaa tggattcggc cgccgagcgc acgagcggct
    12001 agtatcgacg ggccgcatgt gcggcgatgg tggctgtagt tcagttggta gagcaccagg
    12061 ttgtgatcct ggctgtcgcg ggttcgagtc ccgtcagcca cccc
//
LOCUS       DASYLX010000758         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3003732,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000758 DASYLX010000000
VERSION     DASYLX010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003732"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGP27_17825"
     CDS             <1..370
                     /locus_tag="VGP27_17825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853189.1"
                     /translation="HTIVKPSDEEFKERQLPVTMTKLRFFKFIMDEIFPGGDLPQVNV
                     VENHATKAGFKVELVQPLRLHYAHTLDIWSAALEARKDEAIAIQSQEVYDRYMRYLTG
                     CADLFREGYTDVCQFTLTKA"
     gene            413..976
                     /locus_tag="VGP27_17830"
     CDS             413..976
                     /locus_tag="VGP27_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853190.1"
                     /translation="MTSGDGYWLRTSTAKALIFSALAMCLVLGTGFIVIDTSHRAGHG
                     PIGDSAPMTDDQSTDQVVDSTRQIVDAARLQSVSGSSIFLSCTSLRDPPFQAAVYLNF
                     KLPDANSVKHIREVAAAMLAHGWQEAPSMGEHFGFKLTKDGVTSTFHENPDKPGFGAM
                     RIYGECRNTSDHGNDNPAFTDITDRLS"
     gene            complement(969..1478)
                     /locus_tag="VGP27_17835"
     CDS             complement(969..1478)
                     /locus_tag="VGP27_17835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7853191.1"
                     /translation="MGTTEPIIRSATPDDLDAIGVIYAHHVLTGVATFEVDPPDGPEW
                     GRRFGTIVDSGLPFLAAEIGGVVVGYAYCVQWKLRPAYRHTVEDSVYLAPEAAGRGIG
                     GRLLDGLLVRCSDAGVRQVIAVIADSDAYASLALHRNRGFVDAGRLISVGFKHGRWLD
                     TLLLQRSLS"
     gene            complement(1484..1861)
                     /locus_tag="VGP27_17840"
     CDS             complement(1484..1861)
                     /locus_tag="VGP27_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEV7853192.1"
                     /translation="MSGVLFFDGACGMCTRSRDFLLRLNRTGNLRTEPLQAPGTAQRL
                     RISPDRLMEKVWWLEPSGQVYGGAEAANAAVSAALGTSLPLRVYRVPGMKALQDAVYR
                     WVADHRHRFPGTTPYCESHPVAC"
     gene            complement(1986..>2457)
                     /locus_tag="VGP27_17845"
     CDS             complement(1986..>2457)
                     /locus_tag="VGP27_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV7853193.1"
                     /translation="GTHFVQVGLRGYWPPQDTFEWMLEQGMTWHTMQEIWERGFKEVM
                     RDAVAEALAKADKLYVSVDIDVLDPAHAPGTGTPEPGGITSADLLRMVRQLCYEHDVA
                     GVDVVEVAPPYDHAELTVNAAHRVVFEALAGMAARRRDSAPDPTPPGPPARGPA"
BASE COUNT          444 a          813 c          792 g          408 t
ORIGIN      
        1 gcacacgatc gtcaagccca gcgatgagga gttcaaggaa cgccaactcc ccgtcacgat
       61 gaccaagctg cggtttttca agttcatcat ggacgagatc ttccccggcg gagacctgcc
      121 tcaggtcaac gtcgtcgaaa accacgccac caaggccggc ttcaaggttg agctcgtgca
      181 gccgctgcgc ttgcactatg cgcatacttt ggacatctgg tcggccgcac tggaggcgcg
      241 caaggacgag gcgatcgcca ttcagtccca ggaggtctac gaccggtaca tgaggtacct
      301 gaccggttgc gcggatctct tccgcgaggg ctacaccgac gtctgccaat tcactctgac
      361 caaggcctag ctcttacggt cttgctcatc gccgcccgcg tactttgacc gtatgaccag
      421 cggagacggt tactggcttc ggacttcgac cgccaaagcg ctgatatttt ccgcgctcgc
      481 catgtgcttg gtcctcggca ccggcttcat cgtcatcgac acgtcgcaca gggccggaca
      541 tggcccgatc ggcgactccg ccccgatgac tgacgatcag tccacggacc aagtggtgga
      601 ctcgaccagg cagatcgtcg atgcggcccg gctgcaatct gtcagcggca gctccatctt
      661 cttgtcctgc accagcctgc gcgacccgcc gtttcaggcc gccgtttatc tcaacttcaa
      721 gctgcccgat gccaattcgg tcaagcacat ccgtgaggta gcggccgcca tgttggcgca
      781 cggctggcag gaggcacctt cgatgggcga gcacttcggc ttcaagctca ccaaagacgg
      841 tgtcacgtcg acatttcacg agaaccccga caaaccaggt ttcggagcca tgcgcatcta
      901 cggcgaatgc cgcaacacct cggatcacgg caacgacaat ccggccttca ccgacatcac
      961 cgaccgcctc agctgaggct gcgctgaagc agcagggtgt ccagccagcg gccgtgtttg
     1021 aagccgaccg agatgagccg gcccgcgtcg acgaaacccc gattgcggtg cagcgcaagg
     1081 gaagcgtagg catcggagtc tgcgatgacg gcgatgacct gtcgaacacc ggcatctgag
     1141 caacgcacca acagaccgtc gagaaggcgg ccgccgatgc cgcggcccgc ggcctcgggc
     1201 gcgaggtaga ccgagtcttc gaccgtatgc cggtaggccg gtcgcaactt ccactgcacg
     1261 caatacgcgt agcccacgac gacgccgccg atctcggcgg cgagaaacgg cagcccgctg
     1321 tcgactatgg tgccgaaccg gcggccccat tccggaccgt ccggaggatc cacttcgaat
     1381 gtggcgacgc cggtcagcac gtggtgggcg tagatgaccc caatcgcatc gaggtcgtcc
     1441 ggcgtcgcag accggatgat cggttcggtc gtgcccaact cggtcagcag gcgaccggat
     1501 gcgactcgca gtacggcgtt gtgccgggga agcggtggcg atggtcggcg acccatcggt
     1561 agacggcgtc ctgaagagcc ttcatgccgg gaacccgata cacccgcagc ggaaggctgg
     1621 taccgagcgc cgccgacacg gccgcgttgg cggcctccgc accgccgtac acctggccgg
     1681 acggctcgag ccaccacacc ttctccatga gacggtcggg tgaaatcctc agccgctgcg
     1741 ccgtgcccgg cgcctggagc ggttcggttc gcaagttgcc cgtgcggttg agtctgagca
     1801 ggaaatccct ggatcgggtg cacatcccgc acgcgccgtc gaagaagaga actccactca
     1861 tgacctcatt gtcctccggt gacagctcag ccaccgcttg ccgggcggat gtgaccgaac
     1921 tcatccgcgg cgacgctttg gccgggatag caagcgccgc aggggttccg tcggggggca
     1981 gccggtcaag caggtccacg ggcgggcgga ccgggcggcg tcgggtcggg cgcggagtct
     2041 cgacggcggg cggccatgcc cgcgagggcc tcgaagacca cccggtgcgc ggcattgacg
     2101 gtgagctcgg cgtggtcgta ggggggcgcg acctcgacca cgtcgacgcc ggcgacgtcg
     2161 tgctcgtagc acagctgacg aaccatgcgt aggaggtcgg cgctggtgat cccgcccggc
     2221 tccggggtgc ccgtgccggg cgcgtgtgca gggtcgagga cgtcgatgtc gaccgacaca
     2281 tagagtttgt cggccttggc gagcgcttcg gcgacagcgt cgcgcatgac ctccttgaag
     2341 ccgcgctccc agatctcctg catggtgtgc cacgtcatgc cctgctcgag catccattcg
     2401 aaggtgtcct gcggcggcca gtatccgcgc aggccgacct ggacgaagtg ggtgccg
//
LOCUS       DASYLX010000759         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3222995,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000759 DASYLX010000000
VERSION     DASYLX010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222995"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGP27_17850"
     CDS             complement(<1..549)
                     /locus_tag="VGP27_17850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853194.1"
                     /translation="MKGFDGKVAVVTGAGSGIGQALAIELGRSGAKLAISDVDTEGLA
                     ATEERLTAIGAPVKTDQLDVTEREAFELYADAVKEHYGTVNQVYNNAGISFMGSIEAS
                     QFKDIERVMDVDYFGVVNGTKVFLPHLIASGDGHVINISSVFGLLAVPGQGAYNAAKF
                     AVRGFTEALRQEMLVAGRPVKVT"
     gene            complement(710..1776)
                     /locus_tag="VGP27_17855"
                     /pseudo
     CDS             complement(710..1776)
                     /locus_tag="VGP27_17855"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            1697..1876
                     /locus_tag="VGP27_17860"
     CDS             1697..1876
                     /locus_tag="VGP27_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853195.1"
                     /translation="MTDRGNRLLRWSGTHNESALAAWAVIMLLRTIGFGPNRLLIRYS
                     TTPTPNFAIAHHLRR"
     gene            complement(1944..>2148)
                     /locus_tag="VGP27_17865"
     CDS             complement(1944..>2148)
                     /locus_tag="VGP27_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853196.1"
                     /translation="EQFFWVQEEPANQGAWPTFGLTLPELLPDKLPGLKRISRRAMSA
                     PSSGSSKVHAVEQQEILDEAFAP"
BASE COUNT          361 a          716 c          691 g          380 t
ORIGIN      
        1 cgtcaccttg accggacggc ccgcgacgag catctcctga cgcagcgctt cggtgaaccc
       61 gcgcaccgcg aacttggccg cgttgtaggc gccctgcccc ggcacagcga gcaacccgaa
      121 gacactggag atgttgatga catgcccgtc gccggaggcg atcaagtgtg gcagaaagac
      181 tttggtgccg ttgaccacgc cgaaatagtc gacgtccatg acccgttcga tgtccttgaa
      241 ctggctggcc tcgatgctgc ccatgaagga gatgcccgcg ttgttgtaga cctggttgac
      301 cgtgccgtag tgctccttga ccgcgtcggc atacagctcg aacgcctctc gctcggtgac
      361 gtcgagctgg tccgtcttca ccggcgcacc gatcgcggtc agccgctcct cggttgcggc
      421 gaggccctcg gtgtcgacgt cactgatcgc cagcttggca ccggagcggc cgagctcgat
      481 ggccagcgcc tggccgatgc cggaaccggc acccgtcacg acggcgactt ttccgtcgaa
      541 gcccttcatg catgcacccc cagatgtcgg atagctcgac aaaaggctag ccggtacccg
      601 cggtcacggt ttcacggggc gggcctcgag cgtgccgatt ttgcactcag ggtcgtggtt
      661 tgacgcagct cacgaccctg gctgcaatat tggcgccgtc aactgagagc tacaactttc
      721 tgagcttgcg caggatctgc acgaacggcg cgcccgccgc cagaccgctc ttccagttcg
      781 cctgcaggaa gctggcatgg gcgaagactc cgtagtccag cccgaagtcc ttccattcgg
      841 caacctggtc gacgacctcg tcgggtgtgc cgttcaaaag gcatgaccgc agcatcgact
      901 ccggcacgtc ggccgtgtac gacagcacgg tccgttcgtc gagaacctgc ggaacgatgt
      961 cctggaagcc gccgaagtca tcgcccatcg ggtgccgccc accgtggcgc cgccactcgg
     1021 cggcgggagc cagtagcgcg aatgccttca gcggcacgga gtcgagcgct tcgtcgacct
     1081 ctccgcggct ccggcccgtg acgacgtaga ggaagttggc ggcgcggatc gagtccgggt
     1141 cccgtcctgc atccgaagcc gctgtacgta ccgcgtccag acccgccttc tagtcgggtc
     1201 ggtggaatat cgccgcggga aaccatccgt cggcgtagcg tccggtcgcg cgcagcatgc
     1261 gggggccatg cgaggcgatc cagatctgcg gccatttccc cttgtacggc gggaggtcga
     1321 aagtgacgtt gtgcagcggg aagaactcgg aatcgcgggt gaccggttgc ccgttggatt
     1381 gccacagcgc gcggatcgtc gcgaccgcct cgatgaatcg gcccaccggc ttcgaccagt
     1441 cgaccccgta gggctcgttg ccttctcgct cgcggtgccg atgccgagga tcgcgtggcc
     1501 cttcgacagc agatgcaggg tagccgccgc ctgagcggtg accgcggggt tgcgccgacc
     1561 ggtgtcggtt acggctacac caaggtccat ccgccgcagg cggttcttgg tggcgatgtg
     1621 cccgagcatc gtccacggct cgaggcacgc gtcaggcctc ggggtcagcg ccgcagcgcc
     1681 catgtatggc ttcttgatga ctgaccgagg caacaggcta ttgagatggt cgggcaccca
     1741 caacgagtcc gccctggccg cctgggccgt catcatgctg ctgcggacca tcggtttcgg
     1801 accgaaccga ctgttgatca ggtactcgac cacgccgacg ccgaacttcg ccattgcaca
     1861 ccaccttcgc cgatgagctc attgtcggcg aggggcgtct tgagaggttg agtagacggc
     1921 tacttcagtg caggcggcac aggttagggg gcgaacgcct cgtcgaggat ctcctgctgc
     1981 tcgacggcgt gcaccttcga cgaccccgac gacggcgccg acatcgcacg ccgcgaaatc
     2041 cgcttgagcc ccggcaactt gtccggcagc aactcgggca gcgtgaggcc gaacgtcggc
     2101 cacgcaccct ggttggcagg ctcctcctgc acccagaaga actgctcg
//
LOCUS       DASYLX010000760         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_262450,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000760 DASYLX010000000
VERSION     DASYLX010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262450"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VGP27_17870"
     CDS             complement(<1..1901)
                     /locus_tag="VGP27_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7853197.1"
                     /translation="MTTTAEHVRNTLDGRWRDVKNRMRQELSTEIFKPHYTPNTVIAR
                     TKVAEQLKIMANRGAAEDGFKKEHGGNGDVGAAVTQIEMLAMSDLSLMVKAGVQWGLF
                     GGAIENLGTERHHKKYVKKLIDLDLLGCFAMTETGHGSDVQAIETTATYDASTQEFVI
                     DSPTRTSRKDYIGGAAETARVAAVFAQLTTPDGQGHGVHCFVVPIRDDEGNDLPGVTT
                     SDCHYKGGLPGVDNGRIQFDQVRVPRENLLNKYADVAEDGTYSSPIENPNRRFFTMLG
                     TLIRGRVTVGGSAGAAARVALDIATRYALERRQFSAPGDEDEVLIMDYLVHQRRLFPL
                     IAKSYALQFAQNELVAKCHELQTSDDPDAEEQRELESRAAGLKAANTWHATRAVQEAR
                     EACGGAGYLAENRLIALKADTDVFTTFEGDNHVLTQLVAKELLTAYADDIKGMSPVEW
                     VRFAVNFAGERVLKRTAAETIMQTVLDTRQDNEEEGSLFNRGTQVKMFEDREEYMLAT
                     VARRLQGKSKEMSAFDAFNSVQDHVLHTARTHIDRIILEAFVAGIDSCEDDEARKILN
                     MVCDLYALSVIEEDRAWFMEHRFLSTERAKAVTRGINERCRDLRPYAELLVDGFGIPE
                     QLRYAEMLHP"
     gene            2003..2734
                     /locus_tag="VGP27_17875"
     CDS             2003..2734
                     /locus_tag="VGP27_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV7853198.1"
                     /translation="MAESNKAALSPTGMPHVGGHRHSDVSGGWLRAATFGAMDGLVSN
                     TALIAGVAAAASTQTVVISGVAGLLAGAFSMALGEYTSVTTANEQIDSEVRVERRAFE
                     KHPKAERAELVAMLVNLGMSRETAAQATDEIHRDETRAVNFHLVQELGVNPSEKPSPW
                     IAAISSFVMFAIGAVVPLLPYLLGFESLAAGLVCGGIGLAIAGGIAARFTSKPLWWAS
                     LRQLVFGGIAIAATYVVGTLVDSML"
     gene            complement(2740..>3001)
                     /locus_tag="VGP27_17880"
     CDS             complement(2740..>3001)
                     /locus_tag="VGP27_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853199.1"
                     /translation="PVERGVASAAYSFVRFSGAAVAPWLAGVLGEQVSVHLPFWVGAA
                     AVLLGAGVLAMTRRHLTGIDIEESELDGVTDHAESITVGSES"
BASE COUNT          473 a          972 c          987 g          569 t
ORIGIN      
        1 ggatgcaaca tctcggcgta gcgcagttgt tcggggattc cgaacccgtc gacgagcaac
       61 tccgcgtagg gccgcagatc gcggcaccgc tcgttgatgc cgcgggtcac cgccttggcg
      121 cgttcggtgg acaggaagcg atgctccatg aaccacgccc tgtcctcttc gatcaccgac
      181 agcgcataca gatcgcagac catgttgagg atcttgcggg cttcgtcgtc ctcgcaggag
      241 tcgatgccgg ccacgaacgc ttcgagaatg atccggtcga tgtgtgtgcg cgccgtgtgc
      301 agcacgtggt cctgcaccga gttgaacgca tcgaacgcgc tcatctcctt cgacttgccc
      361 tgcagccgac gggccacggt cgcgagcatg tactcctcgc ggtcctcgaa catcttcacc
      421 tgggtgccgc ggttgaatag actgccctcc tcctcgttgt cctgccgcgt atccaggacg
      481 gtctgcatga tcgtttcggc cgcagtgcgt ttcagcaccc gctcaccggc gaagttgacg
      541 gcgaaccgca cccattccac cgggctcatg cccttgatgt cgtcggcgta ggcggtcagc
      601 agctccttgg ccaccaactg ggtcagcacg tggttgtcgc cctcgaatgt ggtgaacacg
      661 tcggtgtcgg ccttcagtgc gatcaaccgg ttctcggcga gatatcctgc gccgccgcat
      721 gcttcgcgcg cctcctggac ggcgcgggtc gcgtgccagg tattggccgc cttcaggccc
      781 gcggcacggg actccagctc gcgctgctct tcggcgtcgg ggtcatccga cgtctgcagc
      841 tcgtggcact tggcaacgag ctcgttctgc gcgaactgca gcgcatacga cttggcgatc
      901 aacgggaaca accggcgctg gtgcaccagg tagtccatga tcagaacctc gtcctcgtcg
      961 ccgggggcgg agaactgcct gcgctccaac gcgtatcggg tcgcgatgtc cagcgccacc
     1021 cgagccgccg caccagcgct gccgcccacg gtgacccgac cgcggatcaa ggtgccgagc
     1081 atcgtgaaga acctgcggtt cgggttctcg atcggtgagg agtacgtgcc gtcctcggcg
     1141 acgtcggcgt acttgttcag caggttctcg cgcggaaccc gcacctggtc gaactggatg
     1201 cggccgttgt cgacaccggg taagccgccc ttgtagtggc agtcagaagt cgtcacgccc
     1261 ggcagatcgt tgccctcgtc gtcgcggatc ggcacgacga agcagtggac gccgtgacct
     1321 tgcccgtcgg gagtggtgag ctgcgcgaaa acagctgcca cacgcgcggt ttcggcagca
     1381 ccgccgatgt agtccttgcg ggaggtgcgg gtgggggagt cgatgacgaa ctcctgtgtc
     1441 gacgcgtcgt acgtcgccgt cgtctcgatg gcttgcacgt cgctgccgtg accggtctcg
     1501 gtcatcgcaa agcagccgag caggtcgagg tcaatgagct ttttgacgta cttcttgtga
     1561 tgccgctcgg tgccgaggtt ctcgatcgca ccgccgaaca agccccactg cacgccggcc
     1621 ttgaccatca gcgacaggtc ggacatcgcg agcatctcga tctgggtgac cgccgcgccg
     1681 acgtcgccgt tgccgccgtg ttccttcttg aagccgtcct cggcggcgcc ccggttggcc
     1741 atgatcttca actgctcggc caccttggtg cgggcgatga cggtgttggg cgtgtagtgc
     1801 ggtttgaaga tctcggtgga cagctcttgc cgcatccggt tcttcacatc gcgccagcgg
     1861 ccgtccagcg tgttgcgtac atgctccgca gtggtggtca tacccagacg gtagcccgtt
     1921 ggcgctcggc ggaaactgtg acttggcaaa cgtaggcaaa cctggctaga cctggcgaaa
     1981 ccgatgccgt tgaatcggtg ccatggccga gtcgaacaag gcggcgctat cgccgaccgg
     2041 gatgccccat gttgggggac accggcactc ggatgtgtcc ggtgggtggt tgcgcgcggc
     2101 caccttcggc gcgatggacg gtctggtcag caataccgcc ctgatcgcgg gggtagcggc
     2161 ggcggcaagt acccaaaccg tcgtcatcag cggtgtggcg ggcctgctgg ccggggcgtt
     2221 ctcgatggcg ctcggcgagt acacctcggt taccaccgcc aacgaacaga tcgactccga
     2281 ggtgcgggtg gagcggcggg cgttcgagaa gcatccgaaa gcggagcggg ccgagctggt
     2341 ggcaatgctg gtcaatctcg gcatgagccg cgagaccgcc gcgcaggcca ccgacgagat
     2401 ccaccgcgat gagacccggg cggtgaactt ccatctcgtt caggagctgg gggtcaaccc
     2461 gtccgagaag ccgtcgccgt ggatcgcggc catctcctcg ttcgtcatgt tcgcgatcgg
     2521 tgccgtcgtg ccactcctcc cgtatttgct cgggttcgag tcgcttgcgg ccggattggt
     2581 ctgcggcggt atcggcttgg caatcgcggg cggcatcgcg gcccggttca ccagcaaacc
     2641 cttgtggtgg gcatcactgc ggcagctggt gttcggcggg atagcgatcg ccgccacgta
     2701 tgtggtcggc acgctcgtcg attcgatgct gtagccggat cagctttcgc tgccgacggt
     2761 gatcgactcc gcgtggtcgg tgaccccgtc gagctcgctt tcctcgatgt cgattccggt
     2821 gaggtgacgt cgcgtcatcg cgagcacacc cgcgccgaga agaaccgcgg cggcgcccac
     2881 ccagaacggc aggtgcacgc tgacctgttc accgaggacg ccggcaagcc acggcgccac
     2941 cgcggctccg gagaaccgca cgaaactgta tgccgccgaa gccactccgc gttccaccgg
     3001 c
//
LOCUS       DASYLX010000761         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2783871,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000761 DASYLX010000000
VERSION     DASYLX010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783871"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGP27_17885"
     CDS             <1..556
                     /locus_tag="VGP27_17885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV7853200.1"
                     /translation="AALTLYPMGQLWWGSFAVSVFVLADMLDGAMARQSGGGSRFGAV
                     LDATCDRIGDGAVFCGLLWWAAFGMRSTSLVVALMICLVTSQVISYVKARAEASGLSG
                     AGGLIERPERLVIVLNGAGLSGISFLHIPWLLHVAAWLLAVASLITLGQRVHTVRTSP
                     GAMEPLQKADSEFTGNSGEQGDEP"
     gene            604..1533
                     /locus_tag="VGP27_17890"
     CDS             604..1533
                     /locus_tag="VGP27_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEV7853201.1"
                     /translation="MRVGGSRFSLGGQIADLGYATGWRVVRAMPELVARNAFDAGAIY
                     ASRNGGPAQLRKNLARVLGVPPARVPGGLIRASLASYARYWREAFRLPSMDKEKLRDR
                     LEPCLLGREHVRAALDAGRGVIMPLPHSGNWDMAGVWMVAHFGTFTTVAERLKPESLY
                     QRFIDYRESLGFEMLPLTGGERPPFEVLTERLRDNGIVALMADRDLTRSGVQVDFFGE
                     PTRMPAGAAKLAIETGAVVLPTHCYNTRDSWVFDVSSPLDTSSGDVTVITQAMADRFA
                     AGIAAHPEDWHMLQPQWLADLSEERQARLRGDS"
     gene            1533..>1961
                     /locus_tag="VGP27_17895"
     CDS             1533..>1961
                     /locus_tag="VGP27_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7853202.1"
                     /translation="MRIGMVCPYSFDVPGGVQSHVLQLAEVMRERGHEVSVLAPSSPH
                     VELPDYVVSGGKAVPIPYNGSVARLRFGPATHRKVKRWLTDGEFDVLHLHEPNAPSLS
                     MLALQAAEGPIVATFHTSTTKSLTLSVFQGILRPYHEKIIG"
BASE COUNT          291 a          626 c          694 g          350 t
ORIGIN      
        1 cgccgccttg acgctgtacc cgatggggca gctgtggtgg ggctcgttcg cggtgtccgt
       61 cttcgtactg gccgacatgc tcgacggcgc gatggcccga cagagcggcg gcggctcccg
      121 gttcggcgcg gtgctcgacg ccacgtgcga ccggatcggc gacggcgcgg tgttctgcgg
      181 gctgctgtgg tgggcggcgt tcgggatgcg cagcacatcg ctcgtggtgg ccctgatgat
      241 ctgcctggtc acctcgcagg tgatctccta tgtcaaggcc cgcgccgagg ccagcggact
      301 gagcggggcg ggtggactga tcgagcggcc cgaacgactg gtcatcgtgc tcaacggcgc
      361 ggggctgtcg ggcatttcgt ttctgcacat accgtggctg ctgcatgtcg ccgcgtggct
      421 gcttgcggtg gcgagcctga tcaccctggg ccaacgcgtg cacaccgttc gcacctcccc
      481 cggggcgatg gagccgctgc agaaggccga ttcggagttc acaggcaatt caggcgagca
      541 gggggatgaa ccgtgaccac caccccgacg ggaccgcagg cggaagaacc tgttgctcct
      601 cctttgcgcg tcgggggtag ccgcttctcg ctcgggggcc aaatcgctga tttgggctac
      661 gccaccggct ggcgagtcgt gcgcgcgatg ccggagttgg tggcgcgcaa cgctttcgac
      721 gccggcgcca tctacgcgtc ccgtaacggg ggcccggcgc agctccggaa gaacttggcc
      781 cgcgtgctcg gagtgccgcc ggccagggtg ccgggcgggc tgattcgtgc ttcgctggcc
      841 tcttacgccc gatattggcg ggaggcattc cgattgccgt cgatggacaa ggagaagctg
      901 cgcgatcggc tggagccgtg tctgttgggg cgcgagcacg tccgggccgc cctcgacgcc
      961 ggccgagggg tgatcatgcc gctgccgcac agcggcaact gggatatggc cggggtgtgg
     1021 atggttgcgc acttcggaac cttcaccacc gtcgccgagc ggctcaagcc ggaatccctg
     1081 tatcagcggt tcatcgatta ccgggaaagc cttggcttcg agatgttgcc gctcaccggc
     1141 ggtgagcgcc cgccgttcga agtgctcacc gagcggttgc gcgacaacgg aatcgtcgcc
     1201 ctgatggccg atcgcgacct cacccgatcc ggcgtgcagg tcgacttctt cggggagccc
     1261 acccggatgc ccgcgggggc ggcgaaactg gccatcgaga ccggggcggt cgtgctgccg
     1321 acgcactgtt acaacacccg cgacagctgg gtcttcgacg tgtcgtcgcc actggataca
     1381 tcgtcaggcg acgtcacggt gatcacccag gcgatggccg accggttcgc cgcgggtatc
     1441 gcagcccatc ccgaggactg gcacatgctg caaccgcagt ggttggccga cctgtccgag
     1501 gagaggcagg ccaggctcag gggcgattcg tgatgcgcat cggcatggtg tgcccatact
     1561 cgttcgatgt tcccggtggc gtgcaatccc acgtcctgca actcgcggag gtgatgcgcg
     1621 agcgcggtca cgaggtcagc gtgctggccc cgtcgtcgcc gcatgtcgag ctgcccgact
     1681 acgtcgtgtc cgggggaaaa gccgttccca tcccgtataa cgggtcggtc gcgcggctgc
     1741 ggttcggccc ggccacgcat cggaaggtca agcgctggct caccgacggg gaattcgacg
     1801 tgctgcattt gcacgagcca aacgcgccga gcctgtcgat gctggcgctg caggccgccg
     1861 aggggccgat cgttgcgacg tttcacacgt cgacgacgaa gtcgttgacg ctcagtgttt
     1921 ttcagggcat cctgcggccc taccacgaaa agatcatcgg t
//
LOCUS       DASYLX010000762         4435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1579468,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000762 DASYLX010000000
VERSION     DASYLX010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579468"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VGP27_17900"
     CDS             complement(<1..155)
                     /locus_tag="VGP27_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV7853203.1"
                     /translation="MIEETLFDAEEKMEKAVSVARDDLSSIRTGRANPGMFSRLSIDY
                     YGSSTPIT"
     gene            complement(198..953)
                     /gene="pyrH"
                     /locus_tag="VGP27_17905"
     CDS             complement(198..953)
                     /gene="pyrH"
                     /locus_tag="VGP27_17905"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV7853204.1"
                     /translation="MAEPTITNGAGATPIRPMYSRVLLKLGGEMFGGGQVGLDPDVVA
                     LVARQIAEVVRSGVQVAVVIGGGNFFRGAQLQQRGMERTRSDYMGMLGTVMNSLALQD
                     FLEKEGIATRVQTAITMGQVAEPYIPLRAQRHLEKGRVVIFGAGMGLPYFSTDTTAAQ
                     RALEIRAEVVLMAKAVDGVFTADPRTNPDAELITAITHREVIERGLAVADATAFSLCM
                     DNGMPILVFNLLTDGNIARAVAGEKIGTLVTTN"
     gene            complement(1072..1761)
                     /locus_tag="VGP27_17910"
     CDS             complement(1072..1761)
                     /locus_tag="VGP27_17910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853205.1"
                     /translation="MTTQPEQEMFESAYRGDAELFQGFRPPWSIGEPQPEIQELIDAG
                     KVHGEVLDAGCGEAATALRLAELGFTTVGLDSAETAVKLAREEAARRGLTNASFEVAD
                     ISNFTGYDGRFGTIIDSTLFHSMPVELREGYQQSIVRAAAPGASYFVLVFDAAGMPVD
                     SPINAVTEDELRDVVSRYWEVDEIRPARIHAYVPEQFAEFFAGFGGTEIRDEPTGRKS
                     MSAWLLSAHSR"
     gene            1807..2238
                     /locus_tag="VGP27_17915"
     CDS             1807..2238
                     /locus_tag="VGP27_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853206.1"
                     /translation="MNALEDDPPLGYLLYRAHNALRSEVTATVLDPLGLTFPQYICMR
                     ILARFPDRSNAELARDTNVSPQAMNMVLRGLVDRGLVTRPASVASGRSLPAQLTAKGM
                     ELLKRTDSGVRGAERRVMADLTNGQCREFKQILLAIGSDPD"
     gene            complement(2235..4211)
                     /locus_tag="VGP27_17920"
     CDS             complement(2235..4211)
                     /locus_tag="VGP27_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEV7853207.1"
                     /translation="MTMESTELHEGTMGDATPYPLDPLTGAEIESAAAIVVNSQYSTP
                     TTRFVMIQLAEPEKNPELTFEGATAVPRRAFVTMYDAAAKSIYEAVVDLKARVIDFWK
                     PIPGRFPSYLVEHMTGVEEKVRQDPRWQDAMRKRGVTDFSLAMIDPWPAGYYGAQDHY
                     DNSALICRPLTFMRAAPSEHGYARPVEGLIVTFDLDKMEVIGIEDHGVVPLPPTAGNY
                     SEQFMFDPNNRPAFTRFRDDVKSIEITQPDGASFTVDGWKVVWQKWSLRIGFNPREGI
                     TLHEVTYTDRGETRPIIYRASLSEMVVPYGDTAPTHWNKNVFDMGEVGMGFSANPLTL
                     GCDCLGEIHYFDGTVNDSSGNAVTIPNAICMHEEDFGISWKHTDFRTGEVEVRRSRRL
                     VVSMICTVGNYEYGFFWYFYNDASMEVEVKLSGVLTTGAVLDGEEPRWGKMVAPGIYG
                     PNHQHFFNFRLDMSIDGAGNSVYEVDSIPEPDPELNPHRNAWITQDTLVASEGEGARD
                     WNWSTGRYWKVTNPSKCNELGSPVAYKLVPKEIVPVMVQEGSYIYDRARFVQHNLWVT
                     KYDAAERFASGDYMYQSADTQGLPEFVADDAPLENTDVVLWYTLGAHHVVRPEDWPVM
                     PCAYTGFHLKPLGFFDGNPALDLPPSPRKACGHH"
     gene            complement(4218..>4435)
                     /locus_tag="VGP27_17925"
     CDS             complement(4218..>4435)
                     /locus_tag="VGP27_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7853208.1"
                     /translation="GGVYYSFAKSWLTNVGVGDAEWMRWVGSIALGMFVLGVVVYFFG
                     RRSAHKTSAEDALAHLAVLDLKKPEGV"
BASE COUNT          755 a         1470 c         1392 g          818 t
ORIGIN      
        1 gtgatcgggg tcgacgaccc gtagtagtcg atcgagaggc gggagaacat gccggggttg
       61 gcgcggccgg tccggatgga cgacaggtcg tcgcgcgcga cggacaccgc cttctccatc
      121 ttctcctcgg cgtcgaagag ggtttcctcg atcacggctt ctccattttc tgctcctcgt
      181 gcgcgtacca ctccaggtca gttggtggtg accagtgttc cgatcttctc acctgcaacc
      241 gcccgcgcga tattgccatc agtcagaagg ttgaacacca ggatgggcat gccgttgtcc
      301 atgcacaggc tgaatgcggt ggcatcggcg acggccaggc ccctttcgat gacctcgcgg
      361 tgggtgatcg cggtgatcag ttcggcgtcg gggttggttc gcgggtccgc ggtgaagact
      421 ccgtcgacgg ccttggccat caggacgacc tccgcgcgga tctccagcgc gcgctgtgcc
      481 gcagtggtgt cggtggagaa gtacggcagg cccatgcccg ccccgaagat cacgacgcgc
      541 cccttctcca ggtgtctctg ggcgcgcagc ggaatgtacg gctcggcgac ctggcccatc
      601 gtgatagcgg tctgaacccg ggtggcgatg ccttcctttt ccaggaagtc ttgcagcgca
      661 aggctgttca tcacggtgcc gagcatgccc atgtaatcgg aacgggtgcg ctccatgccg
      721 cgctgctgca actgggcgcc gcggaagaag ttgccgccgc cgatgacgac ggcaacctga
      781 acgccgctgc ggaccacctc cgcgatctgc cgcgccacca gggccaccac atcggggtcg
      841 aggcccacct gcccgccgcc gaacatctcg ccgccgagtt tcagcagcac ccgcgagtac
      901 atgggccgga tcggggttgc cccggcgccg ttggtgatgg tgggctccgc catccgactc
      961 ccttcggggg tcctcgcacg agatcgccat cccggacctc gtccaggacg gctacccctc
     1021 atcctgcctc atagcggcct ctcagccact atggggcggg ccgacggcgc gtcagcggga
     1081 atgcgccgac agcagccatg cgctcatcga cttgcgaccg gtgggctcgt cacggatctc
     1141 ggtgccgccg aaccccgcga agaactcggc gaactgctcc ggcacatatg cgtggatgcg
     1201 ggccggcctg atctcgtcga cttcccagta cctggagacg acgtcacgca gttcgtcctc
     1261 ggtgacagcg ttgatgggcg agtccaccgg cataccggca gcgtcgaaca ccaggacgaa
     1321 gtaggaggcg cccggcgccg ccgcccgcac gatcgactgc tggtaaccct cacgcagctc
     1381 gacgggcatc gagtgaaaca gcgtggaatc gatgatggtg ccgaaccggc cgtcgtagcc
     1441 ggtgaagttg ctgatgtcgg cgacctcgaa gcttgcgttg gtcaacccgc gcctggcggc
     1501 ctcctcgcgg gccagcttga cggcggtctc cgccgagtcg agcccgaccg tggtgaagcc
     1561 cagctcggca aggcgcaggg ccgtcgcggc ctcgccgcag ccggcgtcga gcacctcgcc
     1621 gtggaccttg cccgcgtcga tcagctcctg gatctcgggc tgaggttcac cgatgctcca
     1681 cggcggacgg aagccctgaa acaactcggc gtcaccgcgg taagccgatt cgaacatctc
     1741 ttgctctggt tgcgttgtca taccccgtgt ctatcaacta agttgatata tgtcaatata
     1801 gttgatatga acgcgctcga ggacgacccg ccgctcggtt atctgctgta ccgggcgcac
     1861 aacgcgctgc gttccgaggt gaccgccacc gtgctggacc cactcggtct cacgttcccg
     1921 cagtacatct gcatgcggat cctggcgcgc ttccccgacc gctcgaacgc cgagttggcg
     1981 cgtgacacca acgtatcccc gcaggccatg aacatggtgc tgcgcgggct cgtcgaccgc
     2041 gggctggtca ccaggcccgc gtcggtggcg tcggggcggt cgctgcctgc tcagctgacc
     2101 gccaagggca tggagcttct caagcgcacc gactccgggg tgcgcggcgc cgaacgtcgc
     2161 gtgatggccg acctgaccaa cgggcaatgc cgggagttca aacagatcct gctggcgatc
     2221 ggctccgacc cggactagtg gtgcccacag gccttccggg gtgacggtgg caggtccagc
     2281 gccgggttgc cgtcgaaaaa gcccagcggc ttgaggtgga agcccgtata ggcgcacggc
     2341 atcaccggcc agtcctccgg ccgcacgaca tggtgggcgc ccagcgtgta ccagagcacc
     2401 acgtcggtgt tctcaagcgg tgcgtcgtcg gcgacgaatt cgggcagacc ctgcgtatcc
     2461 gccgactggt acatgtagtc acccgacgcg aacctctccg cggcgtcgta cttggtcacc
     2521 cacaggttgt gctgaacgaa gcgggcgcgg tcatagatgt aggagccctc ctgcaccatg
     2581 accggcacga tctccttcgg caccagcttg taggccaccg gggagccgag ttcgttgcac
     2641 ttcgacgggt tggtgacctt ccagtaccgg ccggtcgacc agttccagtc ccgcgcgccc
     2701 tccccctcgg aggcgaccag cgtgtcctgc gtgatccatg cgttcctgtg cgggttcaac
     2761 tccggatccg gctcggggat cgaatcgacc tcgtacacac tgtttcccgc tccgtcgatg
     2821 ctcatgtcca ggcggaagtt gaagaagtgc tgatgatttg gaccatagat gccgggcgcc
     2881 accatcttgc cccagcgcgg ttcctcgccg tcgagcaccg caccggtggt cagcacgccg
     2941 gatagtttga cctccacctc catcgacgcg tcgttgtaga aataccagaa gaacccgtac
     3001 tcgtagtttc cgacggtgca gatcatcgac accaccagcc gccgcgagcg acgcacctcg
     3061 acctcgccgg tgcggaagtc ggtgtgtttc caggagattc cgaagtcctc ctcgtgcatg
     3121 cagatcgcat tcggaatggt caccgcattg ccgctggagt cgttgaccgt gccgtcgaag
     3181 tagtggatct cccccagaca gtcgcatccg agcgtaagcg ggttggccga gaaccccatc
     3241 ccgacctcac ccatgtcgaa cacgttcttg ttccaatgcg tcggtgcggt gtcgccatag
     3301 ggcaccacca tctccgagag cgacgcccga tagatgatcg gccgcgtttc accccgatcg
     3361 gtgtaggtga cttcgtgcag tgtgatgccc tcgcgcggat tgaagccgat ccgcagggac
     3421 cacttctgcc aaaccacctt ccatccgtcg acagtgaagc tcgcaccgtc gggttgggtg
     3481 atctcgatgg acttcacgtc gtcgcggaac cgggtgaacg ccgggcggtt gttcgggtcg
     3541 aacatgaact gctcggagta gttgcccgcc gtcggaggca gcgggaccac gccgtgatcc
     3601 tcgatgccga tgacctccat cttgtcgagg tcgaacgtca cgatcaggcc ctccacgggc
     3661 cgcgcatagc cgtgctcgga cggcgccgcc cgcatgaacg tcaacggacg acagatcagc
     3721 gcagagttgt cgtagtgatc ctgcgcgccg taataccctg cgggccatgg gtcgatcatc
     3781 gcgaggctga aatcggtgac gccacgcttg cgcatggcgt cctgccaacg cggatcctgc
     3841 ctgacctttt cctccactcc cgtcatgtgc tcgacgaggt aggacgggaa gcggccggga
     3901 atcggcttcc agaagtcgat cacgcgagcc ttcagatcga cgacggcctc gtagatcgac
     3961 ttcgccgcgg cgtcgtacat cgtgacgaac gctcgtcgcg gtacggctgt cgccccctcg
     4021 aaggtcaact cggggttctt ctccggctcg gcgagctgga tcatcacgaa cctcgttgtg
     4081 ggggttgagt actgagagtt cacgacgatc gcggcggcag attcgatctc cgcgccggtc
     4141 agtgggtcca aagggtacgg cgtcgcgtcg cccatcgtgc cctcgtgcag ctcagtgctt
     4201 tccatcgtca tggcgagtca gaccccctcg ggtttcttga gatcaagcac cgcaagatgc
     4261 gccagtgcgt cttctgcaga tgtcttgtgc gcggaccgtc ggccgaagaa gtacacgacc
     4321 actccgagaa cgaacatgcc caacgcgatg ctgccgaccc atcgcatcca ctcggcgtcc
     4381 cctactccga cgttggtcag ccaggacttg gcgaacgagt agtagacgcc gccga
//
LOCUS       DASYLX010000763         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3003813,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000763 DASYLX010000000
VERSION     DASYLX010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003813"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VGP27_17930"
     CDS             <1..171
                     /locus_tag="VGP27_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV7853209.1"
                     /translation="YRKHRNEMRKMLFILKGRPSTSPFCTWINDPLEPEELPPDWRPP
                     PEPAPSSDEPPF"
     gene            complement(172..480)
                     /locus_tag="VGP27_17935"
     CDS             complement(172..480)
                     /locus_tag="VGP27_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853210.1"
                     /translation="MKHVLLPAMVAGSIAGVALGSASAAYAAPTGPVSASETVNALQA
                     RGLHVILIKTGTAPLDECAVTSVRPGQTFTRTDSGVPGAGSSIVTTVTAKTVYVDVDC
                     "
     gene            complement(519..1034)
                     /locus_tag="VGP27_17940"
     CDS             complement(519..1034)
                     /locus_tag="VGP27_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV7853211.1"
                     /translation="MAEESSLATVRRARPPKDVRPPELDEVDRRILIALHRDARMPNS
                     ALADAVGIAPSTCHGRVRRLHELGVIRGFYADIDPTAIGLSLQAMISVSLQANARAKI
                     RSFIQQIRRKPQVMDVYFLAGADDFLLHVAARDTEDLRAFVVENLNADSDVAGTQTSL
                     IFEHLRGASPL"
     gene            1127..2242
                     /gene="ald"
                     /locus_tag="VGP27_17945"
     CDS             1127..2242
                     /gene="ald"
                     /locus_tag="VGP27_17945"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV7853212.1"
                     /translation="MRVGIPTEIKNNEFRVAITPAGVAELTHRGHEVLIQAGAGEGSA
                     ISDADFKRAGAQLINSADEVWAEAELLLKVKEPIEPEYSRMHEGQTLFTYLHLAASRP
                     CTDALMASGTTSIAYETVQTADGALPLLAPMSEVAGRLSAQVGAYHLMRSHGGRGVLM
                     GGVPGVAPAEVVVIGGGVAGYNAARIAKGMGAHVNVFDVNVNTLRKIDNESSGSIETR
                     YSSMLDLEDAVKDADLVIGAVLVPGAKAPKLVSNSTVAHMKSGAVLVDIAIDQGGCFE
                     DSHPTTHDDPTFAVHDTVFYCVANMPGAVPRTSTYALTNATMPYVVKLAERGWQAACQ
                     ADSALAKGLSTHEGALLSEQVASDLGLPFTDPATVLA"
     gene            complement(2247..>2544)
                     /locus_tag="VGP27_17950"
     CDS             complement(2247..>2544)
                     /locus_tag="VGP27_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEV7853213.1"
                     /translation="TAEELMRSRYSAYAVGDLDYVWRTWHPRTRPEALTPSDEVWTGL
                     EILDTVAGQQGDDGGEVEFCAHYRRDRRSGTLRERSHFAVRARRWFYVDGEVFG"
BASE COUNT          465 a          857 c          789 g          433 t
ORIGIN      
        1 taccgcaagc accgcaacga gatgcgcaag atgttgttca ttctcaaagg ccgacccagc
       61 accagcccgt tctgcacctg gatcaacgat ccgctggaac ccgaagagtt gccaccggat
      121 tggcggcctc cccccgaacc agcaccatca tcggatgagc caccgttctg atcagcagtc
      181 gacgtcgaca tacacggtct tcgccgtgac ggttgtgacg atgctgctgc cggctccagg
      241 cactcccgaa tccgtcctgg tgaaggtctg accgggccgc accgaggtga cggcacactc
      301 atcgagcgga gccgtgcccg tcttgatgag aatgacgtgt aatccacggg cctgcaacgc
      361 attcactgtc tccgacgccg acacgggacc cgtcggcgcc gcatatgcgg ctgaggcaga
      421 ccccagagcg acaccggcta tcgaaccggc gaccattgcg ggcaacaaga catgcttcat
      481 tgaacttcct ctctatcaat aagagataga gggcggggtt acagcggaga ggcgccccgc
      541 aggtgctcga agatcagcga tgtctgcgtg cccgcgacgt cggagtccgc gttgaggttc
      601 tcgaccacga acgctcgcag atcctcagtg tcacgggcgg ccacgtgcag gagaaagtcg
      661 tcggcacctg ccaggaagta gacgtccatc acctgcggct tgcgccggat ctgctggatg
      721 aagctgcgga tcttggcgcg cgcattggcc tgcaggctga ccgagatcat tgcctgcagt
      781 gaaaggccga tggcggtcgg gtcgatgtcg gcgtagaaac cccgtatgac accgagctcg
      841 tgcagccggc gaacccggcc gtgacacgtc gacggcgcga tacccacggc gtcggccagc
      901 gcgctgttgg gcatgcgggc gtcgcggtgc agggcgatca gaatccggcg atcgacctcg
      961 tcgagctcgg gtggccgaac atccttcggc ggacgtgcgc gacgtaccgt cgccaatgat
     1021 gattcttcgg ccacagctcg atgctatcga atgatcgaca agtctgttgc ttgattaccg
     1081 aatcttcttc acaatttacc tagtccatcg acctccagga gcgatcatgc gcgtcggtat
     1141 cccgaccgag atcaagaaca acgaatttcg agttgccatc accccggccg gtgtggccga
     1201 gctcacccat cgcggccatg aggtgctcat tcaggccggc gcgggcgaag gctctgcgat
     1261 ctctgacgcc gacttcaagc gcgcgggcgc ccagttgatc aacagcgccg acgaggtatg
     1321 ggccgaggcc gagcttctgc tgaaggtcaa ggagccgatc gagcccgagt acagccgcat
     1381 gcacgagggc cagacgctct tcacctacct gcatctggcc gcatccaggc cgtgcaccga
     1441 cgcgttgatg gcttcgggca ccacgtccat cgcctatgag accgtgcaga ccgccgacgg
     1501 tgcgctaccg ctgctggccc cgatgagcga ggtcgcaggg cggctctcgg cgcaggtcgg
     1561 cgcctaccac ctgatgcgca gccacggcgg ccgtggcgtt ctgatgggcg gcgtcccggg
     1621 tgtcgccccg gccgaggtcg tcgtcatcgg cggcggcgtg gccggctaca acgccgcccg
     1681 catcgccaag ggtatgggcg ctcacgtcaa cgtgttcgac gtcaacgtca acacgttgcg
     1741 caagatcgac aacgagagca gcggatccat cgagacccgc tactcgtcga tgctcgacct
     1801 cgaggacgcc gtcaaggatg ccgacctggt catcggcgcc gtgctggtgc ccggggcaaa
     1861 ggctcccaag cttgtgtcga attcgacggt cgcccatatg aagtcgggcg cggttcttgt
     1921 cgacatcgcg atcgaccagg gtggctgctt cgaggactcg cacccaacca cgcacgacga
     1981 cccgacgttc gccgtgcacg acaccgtgtt ctactgcgtg gccaacatgc ccggcgcggt
     2041 gccgcgcacc tcgacatacg cgctgacgaa cgccaccatg ccctacgtgg taaagctggc
     2101 cgagcgggga tggcaggccg cttgccaggc tgattccgct ttggccaagg gtctttcgac
     2161 ccacgagggc gcgctgctct ccgagcaggt ggcatccgat ctggggctgc ccttcaccga
     2221 cccggccacc gtgctggcct gaccggtcaa ccgaacacct ccccgtccac gtagaaccaa
     2281 cgacgggctc taacggcgaa gtgggaccgc tcccgaagcg tcccacttcg tcggtcccgg
     2341 cgatagtgcg cgcagaactc cacctctccc ccgtcgtcgc cctgctgccc cgccaccgtg
     2401 tcgaggatct ccagtcccgt ccacacctca tcggacggcg tgagcgcctc gggccgggtg
     2461 cgcggatgcc atgtccgcca gacgtagtcg aggtccccga ccgcgtacgc gctgtagcgc
     2521 gatcgcatca actcttcggc ggtc
//
LOCUS       DASYLX010000764         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3223062,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000764 DASYLX010000000
VERSION     DASYLX010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223062"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..633)
                     /locus_tag="VGP27_17955"
     CDS             complement(79..633)
                     /locus_tag="VGP27_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853214.1"
                     /translation="MGTIARLLGIAVVVVGAALSTAGPSSADDVMEGIYTFHADGSPA
                     AEWTIYPLCTPTVGDLRVPLELPVACRLKVVSATPAKNTFELNNLNWGGDATLTSGIW
                     ALTANKTDGFQCADGSTAPTVDTYTFDDVSLTGTHTSLHTAVCGAAPGMTKTPFTLAF
                     EKPLPIPVDRYPLVCEPAGNRLCR"
     gene            750..1451
                     /locus_tag="VGP27_17960"
     CDS             750..1451
                     /locus_tag="VGP27_17960"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7853215.1"
                     /translation="MTRPVPVFALCVAIAFGVLTTGCTSTVQGAAVKPRSSVPADNVP
                     ALEDADLEGLMLGNSALNKISGVELESFYSAEEMNDNADLVTDRDCLGAIYPGEKAVY
                     DNTDWTAIRDELLLEASGEEDGRLVEQTLVLFDTSDKAVEFFEASKDTWRKCAQIKDI
                     EVEGGSWVPDKVQGVDERTMSLKAAVSGSLEGTCQHAMGVVSNLIVEGFSCDADDHDD
                     AQKVASQILKDAAEQ"
     gene            1474..1944
                     /locus_tag="VGP27_17965"
     CDS             1474..1944
                     /locus_tag="VGP27_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853216.1"
                     /translation="MTAIVLLPSCATGDHGSAPPSTSSAAATTTVVLTTSPIPHPKNW
                     FDLQVGDCLTEIPAIEAGAVTTTVVDCATPHQAEVYLLAPLKVDTAIDQVAGEKCAKG
                     FVDYTGQPFNGSAFRVTYLIDSNQDRTTNNPTPSTAICFLQSADGGPLTKSARF"
     gene            complement(1941..>2402)
                     /locus_tag="VGP27_17970"
     CDS             complement(1941..>2402)
                     /locus_tag="VGP27_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853217.1"
                     /translation="VIQYHFGDRDGLLMAVVDKGFGQLLETLRGMEPELASTAGHARA
                     ELVVSAVADAFLSPTSIAALEILIATRSGRADVDQKHLLELFSTLTRLGRYVADGLEP
                     DRADAVGNLIWTALMGAMVAKMAVPGPVDVSRELRALTDVIAAYVDRYRTT"
BASE COUNT          427 a          790 c          775 g          410 t
ORIGIN      
        1 tatctggccg ggagcgacac ggggtacatc accggggcga actggaacgt ggacggcggc
       61 ttcaccgtat agcggtcatc acctgcacag gcggttgcct gccggttcgc acaccagcgg
      121 atagcggtcg acggggatcg gcagcggctt ttcgaatgcg agtgtgaacg gcgtcttggt
      181 catgcccggt gccgcgccgc agaccgcggt gtggagagac gtatgggtgc cggtgagcga
      241 cacgtcgtcg aacgtgtagg tgtcgacggt gggcgccgtg ctgccgtccg cgcactgaaa
      301 cccgtcggtc ttattcgccg taagggccca gatcccgctc gtcagcgtgg cgtctccgcc
      361 ccagttcaga ttgttcagct cgaatgtgtt cttcgccgga gtcgcgctga cgaccttcaa
      421 cctgcaagcc accggcaatt ccagcgggac ccgcaggtcg cccacggttg gtgtgcatag
      481 cgggtagatc gtccactccg cagcaggcga accgtcggca tgaaacgtgt agatgccctc
      541 catgacgtcg tccgccgacg acgggcccgc ggtgctcagt gccgcaccga ccacaacgac
      601 ggcaatgccc agcagacgag cgatcgtccc catgtcccct cctcaggcca ttgcaaggag
      661 tatcacagtg ccgcccaccg gcagcaagga tcggccaccg tctggccatc accgccgcag
      721 agagcctgta cgtcttctag actcccgcca tgaccaggcc ggtgccggtg ttcgcgctct
      781 gtgtggctat cgcgttcggc gtgctgacga cgggctgcac cagcaccgtg cagggcgccg
      841 ccgtcaagcc ccgctcctcc gtgcccgccg acaatgtgcc cgcgctcgag gacgccgacc
      901 tggagggtct gatgctcggc aacagcgccc tgaacaagat ctcgggcgtt gagttggagt
      961 cgttctattc cgccgaggag atgaacgaca acgccgatct ggtgaccgat agggattgcc
     1021 tcggcgcgat ctatccgggt gagaaggccg tgtacgacaa caccgactgg accgcgatcc
     1081 gcgacgagct gcttctggag gccagcggcg aggaggacgg gcggctggtc gagcagacgc
     1141 tcgtcctctt cgacacctcc gacaaggcgg tcgaattctt cgaggcgtcc aaggacacct
     1201 ggcgcaagtg cgcgcagatc aaggacatcg aggtcgaggg cgggtcgtgg gtgccggaca
     1261 aggtccaagg cgtcgacgag cgcacgatgt cactgaaagc cgcggtgagc ggctccctcg
     1321 aggggacgtg ccagcacgcg atgggagtgg tttccaacct catcgtcgag ggcttctcct
     1381 gtgacgccga cgatcacgac gatgcccaga aggttgcctc gcagattctc aaggatgcgg
     1441 ccgagcagta ggacggcgcg cgtgtgcgcg tcgatgaccg caatcgtcct gctgccgtcc
     1501 tgtgccacag gcgatcacgg cagcgcaccg ccttcgacat cttctgccgc cgcgacaacc
     1561 accgtcgtcc tgaccacctc gcccatcccg cacccgaaga actggttcga cttgcaggtc
     1621 ggtgactgtt tgaccgagat tcccgcgatc gaggccggcg ccgtgaccac cacggtcgtc
     1681 gactgtgcga cgccgcacca ggccgaggtg tacctcctcg cgccgttgaa ggtggacacg
     1741 gcgatcgatc aggtcgccgg tgagaagtgc gccaaggggt tcgtcgacta caccgggcag
     1801 cctttcaacg gcagcgcctt ccgggtgacc tatctcatcg actcgaatca ggaccggacg
     1861 acgaacaatc ccaccccgag taccgccatc tgtttcctgc agtccgccga cggcgggccg
     1921 ctgaccaagt cggcgcggtt ctaggtcgtc cgataccggt cgacataggc ggcgatcacg
     1981 tcggtcaggg cgcgcagttc tcggctgacg tcgacgggtc ccggcaccgc catcttggcg
     2041 accatcgccc ccatcaacgc cgtccagatc agattgccga ccgcgtcggc gcggtcgggc
     2101 tcgagcccgt cggcgacata tcggccgaga cgggtgagcg tggagaatag ttcgaggaga
     2161 tgcttttggt cgacgtcggc gcgtccggag cgtgtcgcga tcaaaatctc cagtgcggca
     2221 atcgatgtcg ggctcaagaa cgcgtcggcg accgcactga ccaccagctc ggccctcgca
     2281 tggccggccg tcgaggccaa ttccggttcc atgccgcgca gtgtttccag gagttggcca
     2341 aaccccttgt ccaccacagc catcagcagg ccgtctcggt cgccgaagtg atactggatc
     2401 ac
//
LOCUS       DASYLX010000765         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_701783,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000765 DASYLX010000000
VERSION     DASYLX010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701783"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1220)
                     /locus_tag="VGP27_17975"
     CDS             complement(264..1220)
                     /locus_tag="VGP27_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853218.1"
                     /translation="MNTHRVAVLALDDVIPLDLAIPIDVFSQSPYRTRLCGPTSPVRT
                     TAGFTINPAGGLRLLSRADTVVVPGYRPHNAPLDPAVLAALRRAHLRGARMISICIGA
                     FALAQAGILDGRPAATHWRFTDELAEQYPAIDVRADVLYVDDGDVLTSAGVSSGLDLC
                     LHVVRTDLGVEEARRFAQELVAAPHREGNQAQFIARPLQTSGVGPIGELCTWAKENLH
                     EQIALEDMAARCAMSPRTLTRHFREATGTTPLQWLLTMRIDEARQLLEATDLSIDEVA
                     ERAGFGTGLNLRVHFRRRLDTTPTTYRRNFRAPANAARREAV"
     gene            1377..2024
                     /locus_tag="VGP27_17980"
     CDS             1377..2024
                     /locus_tag="VGP27_17980"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7853219.1"
                     /translation="MTTTNRNALLATPDVPWASDAIELVFTQESRSVANHSARSWIFA
                     RMLRDHLNLTHEVDETLLFAATLLHDIGLRRDAREPVRFEIDGADRAAEFLTAHGVGA
                     PEVDKVWEAIALHTSPGIPERRGPLTFLTRAGAGMDFGKFTECISDDQAAAIHEAYPR
                     LAFARSIVDDIVEQIRAIPERGPMFSIGHTLVSERSTPPSVTQLERDAAASRWGE"
BASE COUNT          425 a          679 c          674 g          384 t
ORIGIN      
        1 tcaccgtgtt cgatttcgtg taggggtttc gccagagttc ccgatcgcca agtaaccctc
       61 ccagatcgtc cgtatcgggt acggggtctc gacatcggtt cgacggtgcg tcgtaccgcc
      121 gggtgcgccg tcgtggctga agcgagcctt gcccggctct acctcgccgc cgctcgtgat
      181 catcaccgaa gctgacacct tggcggtgtg ccggatggat tcgaagcgtc tctggagccg
      241 ccatcaggtg atcggtgctc gcgttagacc gcctcgcggc gggccgcatt ggcaggggcc
      301 cggaagttgc ggcgatacgt ggttggggtg gtgtcgagcc tgcgacgaaa gtgcacccgc
      361 agattcagcc cagtgccgaa tccggcgcgc tcggccacct cgtcaatgga cagatcggtg
      421 gcttccagca gctgccgcgc ctcatcgatg cgcatcgtca gcagccactg cagcggcgtg
      481 gtgcccgtgg cctcccggaa gtgtcgcgtt aacgtccgtg gcgacatcgc acaccgggcg
      541 gccatatctt ccaacgctat ctgctcgtgc aggttttcct ttgcccaggt gcataattcg
      601 cctattggac cgactccgga agtctggagc gggcgggcga tgaattgggc ctggttgcct
      661 tcacggtggg gggccgcgac cagctcctgc gcaaaacggc gagcctcttc gactccgagg
      721 tcggtgcgga ccacgtgcag acacagatca aggccagatg agacgccggc agacgtcagc
      781 acgtcaccgt catcgacgta gagcacatca gcacgcacat cgatcgcagg gtattgctct
      841 gcaagctcat cggtaaatcg ccagtgcgtt gccgccggcc gtccatcgag aatgccagct
      901 tgggcaaggg cgaaggcgcc gatgcagatc gagatcatcc gcgctccacg taggtgggca
      961 cggcgaagcg ctgccagcac tgcgggatcg agcggcgcat tatggggacg gtatccgggc
     1021 accaccacgg tatcggcgcg cgacaagagg cgaaggcccc ctgcgggatt gatcgtgaat
     1081 cccgccgtgg tgcgtacagg tgacgtgggg ccacaaagcc gcgtccggta cggcgattgt
     1141 gagaaaacgt caatgggtat ggctaagtca agggggatga cgtcgtccag agccagaaca
     1201 gccacccgat gggtgttcat gtcgcgattc taccgatgat gacaataccg ccgcgatcgc
     1261 agcggcccgt tccggcgcag gcaaaggcat cgttctggaa atccgttggc tgaaatttat
     1321 tgaacaatga cttatagaca gtggtcaggt gtgcctacgc gagtgaacat gagactatga
     1381 cgacgacgaa ccgcaatgcc ttgctcgcaa cgccggacgt gccgtgggcc tcggacgcaa
     1441 tcgaattggt gttcacccaa gagagccgct ccgtggctaa tcacagtgct cgaagctgga
     1501 tcttcgcccg tatgctccgc gaccacctga acttgaccca cgaggtcgat gagaccctgc
     1561 tctttgccgc gacactgttg cacgacatcg gtttgcgccg cgacgcgcgc gaacctgttc
     1621 gcttcgaaat cgacggagca gatcgtgccg ctgaatttct caccgcccac ggcgtcggcg
     1681 cccccgaggt cgacaaggtg tgggaggcca tcgcgcttca tacctcaccc ggaattcccg
     1741 aacgccgcgg gccgctgacc ttcctgaccc gcgcgggcgc cgggatggac ttcggcaagt
     1801 tcaccgagtg catcagtgac gaccaagcgg cggccatcca cgaggcctac cctcggttgg
     1861 cattcgcacg cagcatcgtc gacgacatcg tcgagcagat ccgggccatc ccggagcgcg
     1921 gaccgatgtt ctccatcgga cacacccttg tcagcgaacg ctcgacaccg ccaagcgtca
     1981 cgcagctgga acgcgacgcc gccgcgtcac gatggggcga ataacggtgg tatgcaacga
     2041 cagtcatatc aattatcgac gaccggggac gtaaccgagt ccgcagcacc cgcgcacttg
     2101 aacagtcatg ctgcgaaacc cacgccgaga cccacagcag acaaaggtgc cacatcgcat
     2161 ga
//
LOCUS       DASYLX010000766         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2017709,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000766 DASYLX010000000
VERSION     DASYLX010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017709"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGP27_17985"
     CDS             <1..270
                     /locus_tag="VGP27_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3153 domain-containing protein"
                     /protein_id="HEV7853220.1"
                     /translation="LSVSFPGEVTSTNGDQVATEVVEWKLKPGVVSTMSAQARYTDPS
                     ARSFTGAAIWLGLASFLVAGILAALAWVNRDQSPRFADPATTSRN"
     gene            364..1449
                     /gene="idsA2"
                     /locus_tag="VGP27_17990"
     CDS             364..1449
                     /gene="idsA2"
                     /locus_tag="VGP27_17990"
                     /EC_number="2.5.1.1"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004161 - dimethylallyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (2E,6E)-farnesyl/geranyl
                     diphosphate synthase"
                     /protein_id="HEV7853221.1"
                     /translation="MDTAAPSAVELADAVTDQLREYLETRRSDCAYIGTDYADLTAAL
                     EEFVLRGGKRLRPAFAYWGWRAVADPNEPVHPSTLRLFAALELLHACALVHDDVIDDS
                     ATRRGLPTVHRLFAQKHRDRSWRGSSEQFGLSAAILLGDLALVWADDIVATADLSPDA
                     QRRVQRVWSDIRTEVLGGQYLDIVAESSGATTVASAMNVNTYKTASYTITRPLQLGVA
                     AAADRPDVQAIFHDVGNDLGVAFQLRDDVLGVFGDPAVTGKPSGDDLRSGKRTVLLAE
                     AIELAAKSDAGAAELLRSSVGTELSDAQVRDLRRVIESVGALAAVEEHIELLTTRSLK
                     AIDAAPIDAQAKAGLAKLARLAANRSA"
BASE COUNT          273 a          580 c          604 g          267 t
ORIGIN      
        1 ttgtccgtgt cgttccccgg cgaggtcacg tcaaccaacg gtgaccaggt ggcgaccgag
       61 gtcgtcgaat ggaaactcaa gcccggcgtg gtgagcacga tgtcggcgca ggcgcggtac
      121 accgatccga gcgcccgctc gttcaccgga gcggcgatct ggttgggtct ggcgtcgttc
      181 ctggtggcag gcatcctggc cgcattggcc tgggtgaacc gggaccaatc cccccgcttc
      241 gccgatcccg ccaccacatc gaggaactag cagcgccctg gcgtgctacg aagcattggg
      301 aacgctctac tctgaaggca ctcgcgggcc agaacttcga atgaaagggg cggggcgctg
      361 agcgtggata cagcggcacc gtcagccgtc gagttggccg acgccgtgac cgatcagctg
      421 cgggagtacc tcgaaacgcg ccgcagcgac tgcgcctaca tcggcaccga ctacgccgat
      481 ctgaccgcgg cgctcgagga attcgtgctg cgtggcggca agcggctgcg gcctgccttc
      541 gcctactggg gctggcgtgc ggtcgccgac ccgaatgagc cggtgcaccc ctcgacgctc
      601 cggttgttcg cggcgctgga actgctgcac gcgtgcgcgt tggtgcacga cgacgtgatc
      661 gacgactcgg ccacccgtcg cggtctgccc acggtgcacc ggctcttcgc ccagaagcac
      721 cgcgaccgca gctggcgcgg atcctccgag caattcgggc tctcggcggc gatcctgctc
      781 ggggacctcg cgctggtatg ggccgatgac atcgtcgcga cggccgacct gagcccggac
      841 gcacagcgtc gagttcagcg ggtctggtcc gacatccgca ccgaggtgct cggcggccag
      901 tatctcgaca tcgtcgccga gtccagcggg gcgacgacgg tggcatcggc gatgaacgtc
      961 aacacctaca agacggcgtc gtacaccatc acccgaccgc tgcagctcgg cgtggccgcg
     1021 gccgccgacc ggcccgacgt gcaggcgatc ttccacgacg tcggcaacga cctcggggtg
     1081 gctttccagc tgcgtgacga tgtgctgggc gtgttcggcg acccggcggt taccggcaag
     1141 ccgtcgggtg acgacctgcg ctcgggtaag cgcaccgtgc tgctcgccga agccatcgag
     1201 ctcgccgcca agtcggacgc cggggccgcc gagctgttgc gctcctcggt tggcaccgaa
     1261 ctctccgatg cgcaggtgcg cgacctgcgc cgggtcatcg agtcggtcgg cgcgctggcc
     1321 gccgtcgaag agcacatcga actgctcacc acacggtcgc tgaaggccat cgacgccgca
     1381 ccgatcgacg cgcaggcgaa ggccgggctg gccaagctcg ccaggttggc cgccaaccgg
     1441 tccgcctgag ccggcggacg tgaggagcaa ttggggcacg cggacgtgag gagcaattgg
     1501 ggcacgcgga cgtgaggagc aattggggca cgcggacgtg aggagcgatt ggggcacggc
     1561 ggacgtgagg agcaattgag cgcagtgacg accttctcgc ggctgtcctc gttcagcaca
     1621 tctccggagg cccgtcccgc tctgctgggc ttcctcggtg ccgcgatgat caccgcgggc
     1681 gggcttggcg cgggcagcac ccgactgcac gatccgcttc tgga
//
LOCUS       DASYLX010000767         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2346821,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000767 DASYLX010000000
VERSION     DASYLX010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346821"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..679
                     /locus_tag="VGP27_17995"
     CDS             11..679
                     /locus_tag="VGP27_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7853222.1"
                     /translation="MRDVLVVDDDFMVAEIHRRFVEQVDGFRAVGVARTGTEALTAAR
                     ELQPQLILLDVYLPDMTGLDVLQQLRSGGDRVGVIMITAARELDTVTGALDGGAADYL
                     IKPFEFDQLKAKLEAFAVRADALESADGVDQSLIDSLFGNAGAPSLQASLPKGLGTET
                     GRLVLDAVTNAGEVSAAECAERVGISRVSARRYLEHYLGTGALEIRLQYGVGRPERRY
                     RVVK"
     gene            complement(754..4002)
                     /locus_tag="VGP27_18000"
     CDS             complement(754..4002)
                     /locus_tag="VGP27_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV7853223.1"
                     /translation="MVSAVSGLPTGTVTLLLADVEGSTRLWETQPQEMTAAIARLDAT
                     LTDLVAAHSGVRPVEQGEGDSFVVAFARASDAVACALALHRAPLAPIRLRIGVNTGEI
                     QLRDEGNYIGPTINRTARLRDLGHGGQTLLSGATEAMVVDHLPEDAWLNDLGTHPLRD
                     LPRPERVLQLCHPDIRNDFPALRRTESENAQRLPAQLTSFIGRERQISEVRELLTANR
                     LVTLTGAGGSGKTRLAIHVASDHLDDVGYVDLAPITDLDLVPVAVSRALGLPDQPGRS
                     TMDSVVDHIGDRRLLLVLDNCEHLLDASGDLIASVLEACPGLTVVTTSREPIGLDGEV
                     TWRVPSLSLTDEAVALFTDRARRVRPGFTVVDDDAVTVTEICKRLDGMPLAIELAAAR
                     VRALSLTEIRDGLHDRFRLLTGGSRRAVRRQQSLEASVDWSHALLTEPERVLFRRLSV
                     FMGGFDLDAAQQVCSGDALPRHQILDQLALLVDKSLVVAESASGRTRYRLLETVRQYA
                     MEKLGESGEADVVRDRHRDYYTSMAEQFDAPTPAGFETLLDEAETEIDNLRAALAWNQ
                     EHGETDRALKLACSLYPVWLVRNRPLEGLAWFDSIGLSDPDADVTAAVRSRSLADSAV
                     LGASTVDAQLIDKAEEALAIARDLDDPVLLARALTSCIAAAAFDVGSALPYIAEALAV
                     ARELGDRWRLSQILAWHSYTACLAGEPVATAAAGEEGEAHAEASGDRFIARMCRFWGR
                     GVASFQRGDVTEAVAVLSDLIVEADAAGDVFHGLLPRIALAHAMLNRGDMAEARQLSA
                     EAVELGSVLGPFVEMWALAPLAQAALGMGDVAAATEAADVAWQRVMAQPELVIANVMP
                     TVELAFVRGDLNEARRLADDSVAPMAGWHRARALTTRAHVAIAQGDLEQGESDAHDAL
                     TMAVDIHVYQVVPDALEILMELGCATGSDREAARFGAAAEAIRLQQGGTVRFTIYEPA
                     YAAAVTKLRDALGDEFDTAWAAGAALSLNEAISYAQRGHGERRRPSTGWASLTPTEHD
                     VVRLVAEGLGNKDIASRLFVSHRTVQTHLTHVYSKLGITSRVALAQEAARHG"
     gene            4154..4588
                     /locus_tag="VGP27_18005"
     CDS             4154..4588
                     /locus_tag="VGP27_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853224.1"
                     /translation="MTAPMTDAPEVAALGQAVHRVARRLRKRADLPLTASQTSALTTV
                     ERHGELRLGELARLEQVGKSTMTRLVSKLEDAGYLHRWVDPTDGRVSLVKVTAHGAAV
                     LREARSRQQAYLLRQFEALDAADQAALLASVTAFEKLLAVKA"
     gene            4585..>5266
                     /locus_tag="VGP27_18010"
     CDS             4585..>5266
                     /locus_tag="VGP27_18010"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853225.1"
                     /translation="MKPYLNATFISLRTRNYRLYFIGQSVSLAGTWMQKMAQAWLVLE
                     LTNSGGWLGITLAVQQLPLLFLAPWGGLLADRMNKRTVLIVTATAAMIPSLLLGVLTL
                     THHINVGLVLGLAVVGGLIDALDKPARQSFPSEMVSAPHLANAVILNNIIQDTGKVVG
                     PAVAGVLIAAVGLPYTLFVNSLSFLAVLAGLLLMRPVDLSAPDRVARGAGQLRAGLAY
                     VRATPRLFG"
BASE COUNT          874 a         1787 c         1707 g          898 t
ORIGIN      
        1 caggccgctc atgcgtgacg tgctggtcgt cgacgacgac ttcatggtgg ccgagatcca
       61 ccgcaggttc gtagagcagg tcgacggctt ccgcgcggtc ggcgtggccc gcacgggcac
      121 agaagcgctg acagcggccc gcgaactgca accgcagctg atcctgctcg acgtctacct
      181 accggatatg accggactgg acgtgttgca acagttgcga tccggcggtg acagggtggg
      241 cgtcatcatg atcaccgctg cacgcgagtt ggacacggtg accggcgccc tcgacggtgg
      301 tgcagccgac tacctgatca agccgttcga attcgaccag ctgaaagcca agctggaggc
      361 gttcgcggtg cgcgccgacg cgctggaatc cgccgatggc gtcgaccaat cactgatcga
      421 ttcgctcttc ggcaatgcgg gagcaccgtc actgcaggcg tcgctaccca agggcctcgg
      481 gacggagacc gggcgcctgg tgctcgatgc cgtcacgaac gccggtgagg tttccgcggc
      541 ggaatgcgcg gagcgcgtcg gtatctcacg ggtcagcgcg cgtcgctatc tcgagcacta
      601 cctcggcacc ggcgcactgg agatccggct gcagtacggc gttggtcggc cagagcggcg
      661 ctaccgcgtg gtgaaatgac gggacccttc gtcgagactg cagatacgca gggaaagttc
      721 gagaacgggc ctgcgtagtt tcagtctcga cggtcagccg tgccgggcgg cctcctgggc
      781 cagcgcgacg cgcgacgtga tgccgagctt tgagtagacg tgggtcaggt gcgtctgcac
      841 ggtgcgatgg gatacgaaca gccgcgaggc gatgtcctta ttgccgagtc cctcggcgac
      901 cagccgaacg acgtcgtgct ccgtcggcgt caaggacgcc caacccgtcg acggccttct
      961 ccgctcgcca tgcccgcgtt gggcgtagga gattgcctca ttcagcgaca gcgccgctcc
     1021 tgcggcccac gcggtgtcga attcgtcacc aagcgcatcg cgaagtttcg tgacggccgc
     1081 cgcgtaggcc ggttcgtaga tcgtgaaccg gaccgtgcct ccttgctgta gccggatcgc
     1141 ttctgccgca gcaccgaacc gcgccgcttc gcggtcactc cctgtcgcac aacccaattc
     1201 catcaagatc tcgagtgcat cgggcacgac ctgatagacg tgtatgtcga cggccatggt
     1261 gagcgcgtcg tgcgcatcgc tttcgccctg ttccagatcg ccctgtgcga tcgcgacgtg
     1321 ggcacgcgtt gtcaatgccc tcgcacggtg ccaacccgcc atcggcgcga ccgaatcatc
     1381 ggccaaccgc ctcgcctcgt tgaggtcacc gcgaacgaat gcgagctcca ccgtcggcat
     1441 gacgttcgcg atcaccagct ccggttgcgc catcacccgt tgccacgcaa cgtcggcggc
     1501 ttcggttgcc gcagcgacgt cgcccatgcc cagcgctgcc tgggccaaag gggccaaggc
     1561 ccacatctcc acaaagggcc cgagcaccga accgagctcc acagcttcgg ccgatagttg
     1621 cctcgcttcg gccatgtcgc cccggttcag cattgcgtgt gcgagggcaa tgcgcgggag
     1681 tagcccgtgg aagacgtcgc cggccgcatc cgcctcgaca atgaggtcac tgagcaccgc
     1741 gaccgcctca gtgacgtcgc cgcgctgaaa gctcgcgact ccgcgacccc aaaaacggca
     1801 catgcgggca atgaagcggt ctccggatgc ttcggcgtgc gcctcgcctt cctcgccggc
     1861 ggcagccgtc gcgaccggct cccccgccag gcacgccgtg tatgagtgcc acgcgaggat
     1921 ctgactcaac ctccaacggt cacccaattc tctggccacg gcgagcgcct cggcgatgta
     1981 cggcagtgcc gatccgacgt cgaatgcggc cgcggctatg caggaggtga gtgcgcgggc
     2041 gagcaggacg ggatcgtcga gatcgcgcgc gatggcgagc gcttcttccg ccttgtcgat
     2101 cagttgggca tctacggtgg aggctcccag cacagcgctg tcggcgagcg accgcgaacg
     2161 gacagccgca gtcacgtctg catccgggtc gctcaagccg atggagtcga accacgccag
     2221 gccctccagc ggtctgtttc gcaccagcca caccgggtac agcgagcacg ccaatttcaa
     2281 tgcgcgatct gtctccccgt gctcctgatt ccatgcgagc gcggcgcgga ggttgtcgat
     2341 ttcggtctcg gcctcgtcca gcagggtttc aaagccagcg ggcgtcggcg cgtcgaactg
     2401 ttcggccatc gacgtgtagt agtcgcgatg gcgatcgcga acgacatcgg cttcgcccga
     2461 ctcgccgagc ttttccatcg catactggcg caccgtttcc aacagccggt atcgcgttcg
     2521 tccgcttgcg ctttcggcga cgaccaacga cttgtcgacg agcaacgcca actgatccag
     2581 aatctggtgg cgcggcagag cgtcgccgct gcatacctgc tgcgcggcgt cgagatcgaa
     2641 cccgcccatg aacaccgaca gccggcgaaa gaggacgcgt tcgggttctg tcagcagcgc
     2701 atgtgaccag tcgacggagg cttcaaggct ctgttggcgc cgcaccgccc gccgcgagcc
     2761 tccggtcagt agccggaagc ggtcgtgtag gccgtcgcga atctcggtaa gcgacaacgc
     2821 gcgaacccgg gcggccgcca gttcgatggc aagcggcatt ccgtcgagcc gtttgcagat
     2881 ctcggtgacg gtgacggcgt cgtcgtcgac gacggtgaat ccgggccgca cccgccgcgc
     2941 ccggtcggtg aacagcgcga cggcttcgtc ggtcagcgac agcgacggca cccgccaggt
     3001 cacctcgccg tcgagaccga tcggttctct gcttgtcgtc accaccgtca ggccggggca
     3061 ggcctcgagc acggaggcga tcaggtcgcc gcttgcgtcg agaaggtgtt cgcagttgtc
     3121 gagcaccagg agcagcctgc gatccccgat gtggtcgacg acgctgtcca tcgtggatcg
     3181 gccgggctga tcgggaagcc cgagcgcgcg cgacaccgcg acgggcacca gatccaggtc
     3241 ggtgataggc gccaggtcga cgtatccgac gtcatcaagg tgatcgctcg cgacgtgaat
     3301 cgccaaacgc gtcttgcctg atccgcccgc gccggtgagt gtgaccagtc ggttcgcggt
     3361 cagaagctcg cgcacctccg agatctggcg ctcacgtccg atgaagctgg tgagctgcgc
     3421 cggaaggcgt tgcgcattct cggattccgt ccttcgcaat gccggaaagt cgttgcggat
     3481 gtccggatgg cacagctgca gaacgcgttc cggtcggggc agatcgcgca gcggatgtgt
     3541 gccgaggtcg ttcagccagg cgtcctcggg caggtgatcg acgaccatcg cctcggtggc
     3601 gccagacagc agcgtctgac cgccgtgacc aagatcccga agccgcgccg tgcggttgat
     3661 cgtcggcccg atgtagttgc cctcgtcacg caactggatc tcgcccgtat tcacgccgat
     3721 gcgcagccgg atcggcgcaa gcggcgcgcg gtgtagcgcc agcgcgcacg cgaccgcatc
     3781 gctggcacgg gcgaaggcga cgacgaagct gtccccctcg ccctgctcga ccgggcgcac
     3841 gccgctatga gcggcgacca ggtccgtcag cgtggcatcc agtcgggcga ttgcggccgt
     3901 catctcctgc ggctgggtct cccagagccg cgtcgacccc tcgacatctg cgagcagcag
     3961 cgtcaccgtt cctgtcggta gcccactcac ggcggagacc atactgctcc tcacatccgc
     4021 gggttcgctc cactcgagtt ggctcatgct agccagcatg cggccgtgac cgggggcgaa
     4081 acatcggcgc aagcgcgtat atctccatgc caccgagaaa gtacgctagg cgtactattc
     4141 ggggcgagac acgatgaccg ctccgatgac cgacgcccct gaggtggccg cccttggcca
     4201 ggcggtgcac cgagtcgcac gccgactgcg caagcgtgcg gacctgcctt tgaccgcttc
     4261 gcagacctcc gcgctcacca ccgtggaacg acacggcgag ctgcggctcg gagagctcgc
     4321 ccggttggag caggtcggca agtcgaccat gactcggctg gtcagcaagc tcgaggacgc
     4381 gggctacctg caccggtggg tcgaccccac agatggtcgg gtatccctgg tgaaggtcac
     4441 cgcccatggc gccgccgtgc tgcgggaggc gcgctcgcgc cagcaggcct acctgctgcg
     4501 gcagttcgag gcactcgacg ccgccgacca ggcggcactg ctggcttccg tcacagcatt
     4561 cgagaagctg ctggcggtca aggcgtgaag ccctacctga acgccacctt catctctctg
     4621 cggacccgca attaccggct gtacttcatc ggccagtcgg tgtcgctggc cggtacctgg
     4681 atgcagaaga tggcgcaggc ctggctggtg ctcgaactga ccaactccgg tgggtggctc
     4741 ggcatcacac tcgccgtgca gcaattgccg ctgcttttcc tcgcgccgtg gggcggtctg
     4801 ctggccgacc gaatgaacaa gcgaactgtt ctgatcgtca cggcgacggc ggccatgatc
     4861 ccgtcgctgc tgctcggcgt tctcacgctg acccaccaca tcaacgtcgg cctcgtgctc
     4921 ggccttgcgg tcgtcggcgg actcatcgac gcgctggaca agcctgcgcg acagtccttt
     4981 ccgagcgaaa tggtgtccgc gccacacctg gcgaacgcgg tcatactgaa caacatcatc
     5041 caggacaccg gaaaggtcgt cggtcccgcg gtcgccggag tcctgatcgc cgccgtcggg
     5101 ctgccgtaca cgcttttcgt caactcactg tcgttcctcg cggtactcgc cggcctgctc
     5161 ctgatgcgac ccgtggacct ttcggctccg gacagagttg cccgtggggc aggccaactg
     5221 cgtgcgggac tggcctacgt gcgcgccacc ccccgactgt tcggcc
//
LOCUS       DASYLX010000768         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1139145,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000768 DASYLX010000000
VERSION     DASYLX010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139145"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VGP27_18015"
     CDS             complement(<1..755)
                     /locus_tag="VGP27_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7853226.1"
                     /translation="MSCQTDGQFDVWRNNVRKSVLGSVDQFVADRCALDLREAGVDVA
                     ADVLVGFVGAGKCLRSTFMFLGWLCGAEPSHAALRAAASLELLHAFALLQDDVMDGSP
                     LRRGRASAHVQFADWHRGRGLSGSAERFGESAAVLLGDLCLVWAEQMMRDSGVDHRAL
                     TRAWPRYDAMRSELAVGQFADLVNDVGGLPTLEEVLDVARRKSGNYTVRRPLEIGAAM
                     AGCGDRLLIRLGRYGDAVGEAFQLRDDLLGIFGS"
     gene            915..1835
                     /locus_tag="VGP27_18020"
     CDS             915..1835
                     /locus_tag="VGP27_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEV7853227.1"
                     /translation="MSEVAVECSVEFPGPASPGHTLSPLRRGGGDPCYLTAPDGAVWR
                     TGLMPTGPVTARISRSGPRTIGCEAWGRGAAEFADGFPALLGADDDAAGFAPEEPTIA
                     EADRRVPHLRLGRTGRVLEALIPAVLEQRVSGKDAFRAWRLLVTKFGSPAPGPAPARM
                     RVPPPAEVWRRIPSWEFHLANVDPARARTVIGCAQRADSLERLVVRPTEQAREALTSL
                     PGVGIWTAAETAQRAFGDADALSTGDYHLAKVVGWSLLGHPIDDDAMVELLAPVRPHR
                     HRAVRLLEVSGLARNPRFGARQAIPNLADL"
     gene            1917..2474
                     /locus_tag="VGP27_18025"
     CDS             1917..2474
                     /locus_tag="VGP27_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV7853228.1"
                     /translation="MTQFTVPGLSEKQGGEVAELLQKALSRYNDLHLTLKHVHWNVVG
                     PNFIGVHEMIDPQVELVRGYADEVAERIATLGKSPLGTPGAIINDRTWDDYSVERDTV
                     QAHLAALDLVYTGIIEDTRKSIDRIEDLDPITEDMLIAHSAELEKFQWFVRAHLENAG
                     GQLANEGAKTEKAAASRAKKKAPVK"
     gene            complement(2467..3252)
                     /gene="aqpZ"
                     /locus_tag="VGP27_18030"
     CDS             complement(2467..3252)
                     /gene="aqpZ"
                     /locus_tag="VGP27_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEV7853229.1"
                     /translation="MTPPTMMHRLTAEFIGTFWLVLGGCGSAVFSAKFESSDGVSLGI
                     GFLGVALAFGLTVLTGVYAFGTISGGHFNPAVTLGAALARRVEWKALPGYWIVQVLGG
                     LVAGLVILVVANGKAGFDATGNMAANGYGAHSPGGYSLLAVAITEVVLTGVFLLVILG
                     STDDRAPASKGFAGLSIGLTLTLIHLISIPISNTSVNPARSTGVAFFNGDGAPAQLWV
                     FWLAPLIGAAIAGLAYPYLFGGGEELADQPVRDDALEDETGTT"
     gene            complement(3341..>3880)
                     /locus_tag="VGP27_18035"
     CDS             complement(3341..>3880)
                     /locus_tag="VGP27_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV7853230.1"
                     /translation="RVPALAKAAIIGAIVGFAMFVYPLAVGGGEDLTQRILGGQHIAV
                     SVVIGILAVRIVAGPLSYSAAVPGGLFAPLLAVGALWGLVFAGGFDAVWPGDARQLAI
                     PMALVGMAAFFGATVRAPVTGMVVVTEMTATTATLVPMMAATAAAVFAAYAAGSAPIY
                     ESLRERMPDETAIERRGNG"
BASE COUNT          714 a         1321 c         1264 g          581 t
ORIGIN      
        1 gagccgaaga tgccgagcag atcgtcgcgc agctgaaagg cttcgccgac cgcgtcgccg
       61 taacggccga gacggatcag caggcgatcg ccgcagcctg ccatcgccgc accgatctcc
      121 aacggccggc gcaccgtgta attgcccgac ttgcgtcgtg ctacgtccag cacctcttcc
      181 agggtaggca ggccgccgac atcattgacc aggtcggcga attgtccgac cgccaattcg
      241 ctgcgcatcg cgtcgtagcg gggccacgcc ctggtcagcg cgcgatgatc gaccccggag
      301 tcccgcatca tctgctccgc ccacaccagg cacaggtcac cgagcagtac cgccgccgat
      361 tcaccgaacc gctctgccga tccggagagt ccgcggccgc ggtgccagtc ggcgaactgc
      421 acgtgcgccg acgcccggcc gcgccgcagc ggcgagccgt ccatcacgtc gtcctgcagc
      481 agcgcgaagg cgtgcaacag ctcgaggctc gcggcggcac gcagcgcagc atgcgacggc
      541 tctgcgccac acaaccagcc caggaacatg aacgtcgagc gcaggcactt accggcgccg
      601 acgaatccga ccagcacatc ggcggccaca tcgactcccg cctcgcgcag atcgagggcg
      661 caacgatcgg cgacgaactg gtcgacagag cccagaaccg acttacggac attgttgcgc
      721 cagacgtcga actgaccgtc ggtttggcaa ctcaccgccg gagcggggtg aagcgcgggc
      781 gcgaaaagcc caccgttgac acccaacccg cacccctttc ccgacccacc agcaggccgc
      841 cagcctattt gcgtcccatc cataccccca gccgaggcgg ccgaaccggt atcaatgtca
      901 ctattacgtc atcaatgtct gaagtagccg tcgaatgcag cgtcgagttc ccgggacccg
      961 cgagcccggg tcacacgctg tctccgctgc gccgtggtgg tggcgacccg tgttatctca
     1021 ccgcacccga cggtgccgtc tggcgcaccg gtctgatgcc caccggaccg gtgacagcac
     1081 gcatcagccg gtcaggtccc cgcaccatcg ggtgtgaggc atggggccgc ggcgcagcgg
     1141 aattcgccga cggtttccct gcactgctgg gcgccgacga tgacgcggct ggattcgccc
     1201 ccgaggaacc gacgatcgcc gaggcggatc ggcgggtgcc ccacctacgg cttggccgca
     1261 ccggtcgggt gctggaggcc ctgattccgg cggtactcga acagcgggtg tcaggcaagg
     1321 atgcctttcg cgcctggcga ttgctggtca ccaagttcgg atcgcccgcg ccgggcccgg
     1381 cgcctgcccg gatgcgcgtt cccccgcccg ccgaggtgtg gcgccgcatc ccgtcatggg
     1441 agttccacct cgccaacgtc gaccctgcaa gggcgcgcac ggtgatcggc tgtgcgcagc
     1501 gcgccgactc gctggagcga ctcgtcgtac ggccgaccga acaggcgcgg gaggcgctga
     1561 cctcgctgcc cggcgtcgga atatggacgg cggcagagac ggcacagcgc gcgttcggcg
     1621 acgccgacgc actgtcgacc ggggattatc acctggcgaa ggtggtcggc tggagcctgc
     1681 tcggtcatcc catcgacgac gacgcgatgg tcgaattgct cgcaccggtc cgtcctcacc
     1741 ggcacagggc cgtgcgactt ctcgaggtca gcggtctggc ccgcaaccca cgcttcggcg
     1801 cccgtcaagc gataccgaat ctggccgatc tgtgacgtgc cccgcggcgt gattaccgca
     1861 ccgccgcccc gggtagtcgt aagcccgacg gttcatcatg tgcgaaggag ctttcgatga
     1921 ctcagttcac ggtcccaggt ttgtcggaga agcagggtgg cgaggtagcc gagttgctgc
     1981 agaaggcact cagccgatac aacgacctgc acctcacgtt gaagcacgtg cactggaacg
     2041 tggtcgggcc gaacttcatt ggtgtgcacg agatgatcga cccgcaggtc gaactcgtcc
     2101 gcggctacgc cgacgaggtc gctgagcgga tcgccacgct gggcaagtca ccgctgggca
     2161 cgcccggtgc catcatcaac gaccggacct gggacgacta ttcggtcgag cgggacaccg
     2221 tgcaggcgca cctggccgct ctcgatctcg tctacacggg aatcatcgag gacacccgca
     2281 agtccatcga ccggatcgag gatctcgacc cgatcaccga ggacatgctg atcgcgcatt
     2341 cggccgagtt ggagaagttt cagtggttcg tgcgtgcaca cctcgagaac gccggcggcc
     2401 aattggccaa tgagggtgcc aagaccgaga aggccgccgc gagccgagcc aagaagaagg
     2461 cgccggtcaa gtagtaccgg tttcatcctc cagggcgtcg tcgcgcacgg gctggtcggc
     2521 gagttcctcg ccgccgccga agagatacgg gtaggcgagg cctgcgatgg ccgccccgat
     2581 cagcggtgcg agccagaaga cccacagctg tgcgggtgcg ccgtcgccgt tgaagaacgc
     2641 gacgccggtc gaacgcgccg ggttcaccga tgtgttggag atcgggatcg agatcagatg
     2701 gatcagcgtc agggtcaagc ctatcgacag ccccgcgaag ccttttgacg cgggtgcgcg
     2761 gtcgtcggtc gagcccagga tgacgagcag gaacacgccg gtgaggacga cctcggtgat
     2821 cgcgacggcc aacagcgagt agccacccgg cgaatgcgcg ccgtacccgt tggctgccat
     2881 gttgcctgtc gcgtcgaagc ccgccttacc gttggccacc accaggatca ccaggccggc
     2941 caccagcccg ccaagcacct gcacaatcca gtagccgggc aacgctttcc actcgacgcg
     3001 gcgcgccagg gcggcgccca gggtgacggc tgggttgaag tggccgccgg agatcgtgcc
     3061 gaaggcgtag acgccggtga gcacggtcag gccgaatgcc agcgcgacac cgaggaaccc
     3121 gatgccgagc gagacgccgt cggacgactc gaacttcgcg gagaagaccg cgctgccgca
     3181 acctccgagg accaaccaga aggtgccgat gaattcggcg gtcaatcgat gcatcatggt
     3241 gggcggagtc atggctgtga gaatggcacg cagatactgc tcggcggatg gattgttgcc
     3301 gaatcgtttc gggaaagaaa ttcgtgatgg acgcgatctg ctaaccgtta ccgcgtcgct
     3361 cgatggctgt ttcgtcgggc atccgctctc gaagactttc atagatcggc gccgatcccg
     3421 cggcgtaggc ggcgaacacc gctgctgcgg tcgcggccat catcggcaca agcgtcgcgg
     3481 tagtcgcggt catctctgtg acgacgacca ttccggtcac cggcgcacga acggtggctc
     3541 cgaagaacgc cgccattccg accagcgcca tcgggatcgc taattgcctt gcgtctcctg
     3601 gccatacggc gtcaaacccg ccggcgaaga cgagtcccca cagcgcgccc accgcgagca
     3661 gtggcgcgaa cagtccgccc ggcaccgctg ccgaatatga cagagggcct gcgacgatcc
     3721 gcacggcgag gattccgatg acgaccgaga cagcgatgtg ctgtccgccg aggatgcgtt
     3781 gcgtcaggtc ctcgccgccg cccaccgcga gcggatacac gaacatggcg aacccgacga
     3841 tggcaccgat gatggctgcc ttcgccagcg ccgggactcg
//
LOCUS       DASYLX010000769         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1247882,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000769 DASYLX010000000
VERSION     DASYLX010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247882"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VGP27_18040"
     CDS             <1..1107
                     /locus_tag="VGP27_18040"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEV7853231.1"
                     /translation="AAMVGSGEQMDFGDLRRDDKPLALVDKHSTGGVGDKITIPLVPV
                     VMACGGAVPQAAGRGLGHTGGTLDKLEAIPGFTAEISKSQIRQQLCELGAAVFAAGEL
                     APADRKIYALRDVTGTTESLPLIASSVMSKKIAEGTRALVLDSKVGSGAFLKTEAESR
                     QLARTMVDLGAEHGLVTRALLTDMNRPLGRTVGNAVEVDESLEVLAGGGPADVVELTL
                     AFAAEMLDAAGLDDVDPAQTLRDGSAMDHFRRLVTAQGGDLAQPLPVAAHSETITAPR
                     DGTMGDIDAMAVGLTVWRLGAGRAVPGERVQHGAGLRIHRRPGEPVAAGEALFTLYTE
                     TPERFGGAMAEMDGAWSVGEAAPDIGPLIIDRIV"
     gene            1109..>2145
                     /locus_tag="VGP27_18045"
     CDS             1109..>2145
                     /locus_tag="VGP27_18045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV7853232.1"
                     /translation="MTSPLTLATVRQAPKALLHDHLDGGLRPATVLDLAEQTGYQDLP
                     ATDADGLAAWFRTAAHSGSLVRYLEPFAHTVGVMQTPEALYRVAYECVEDLAADNVVY
                     AEIRFAPELHIDRGLSLDAVVDAVLAGFADGEKAAGSEGRTITVRCLVTAMRHQARSQ
                     EIAQLAIQFRDKGVVGFDIAGAEAGYPPTRHLDAFEYMRNHNGHFTIHAGEAFGLPSI
                     HEAIAFCGADRLGHGVRIADDITVAPDGTVDLGRLASLLRDKRIPLELCPSSNVQTGA
                     VGSIAEHPFDLLAKCRFRVTVNTDNRLMSDTTMSQEMLRLVEAFGYGWSDLARFTINA
                     MKSAFIPFDQRL"
BASE COUNT          349 a          714 c          748 g          334 t
ORIGIN      
        1 gccgcgatgg tgggctcggg agagcagatg gatttcggtg atctgcggcg cgacgacaaa
       61 ccgcttgcgt tggtggacaa gcactccacc ggcggcgtcg gcgacaagat caccatccct
      121 ctggtgcccg ttgtcatggc gtgcggcggc gcggtgccac aggcagcagg tcgcggcctc
      181 ggacacaccg gaggcacact cgacaagctg gaggccatcc ccggcttcac cgccgagatt
      241 tcgaaaagcc aaattcggca gcaactttgc gaactcggcg cggcggtctt cgccgcaggc
      301 gagctggcac ctgccgaccg caagatctat gcgctgcgcg acgtcaccgg caccaccgag
      361 tcgctgccgt tgatcgccag ttcggtgatg agcaagaaga tcgccgaggg cacccgcgca
      421 ctggtgctcg actcgaaggt cggctcaggg gcgtttctga agaccgaggc cgaatcccgg
      481 caactggccc gaacgatggt cgatttgggc gccgaacacg gcttggtcac gcgtgcgctg
      541 ctgaccgaca tgaaccggcc gctggggcgg acggtcggta acgcggtcga ggtggacgag
      601 tccctcgagg tgctggcagg aggagggccg gccgacgtcg tcgagctgac gctggcgttt
      661 gccgccgaga tgctcgacgc cgcgggcctc gacgacgtcg acccggcgca gacgctgcgc
      721 gacgggtcgg ccatggacca cttccgccga ctggtcaccg cccagggcgg cgatttggcg
      781 caaccattgc ccgtggctgc gcactccgag accatcacgg caccccgcga tggcacgatg
      841 ggcgatatcg acgcaatggc ggtgggactg acggtctggc ggctcggcgc ggggcgtgcc
      901 gttccgggcg agcgcgtgca gcacggcgcc ggcctgcgca ttcatcgccg ccccggcgaa
      961 cccgtcgccg cgggggaggc gctgttcacg ctgtacaccg agacgccgga acggttcggc
     1021 ggcgcgatgg cggagatgga cggcgcctgg agcgtcggcg aggcggctcc cgacatcggt
     1081 ccgctgatca tcgatcggat tgtgtgacat gacgagcccc ctcaccctgg ccaccgtccg
     1141 gcaggcgcca aaggcgctgc tgcacgacca cctcgatggc gggcttcggc cagccaccgt
     1201 gctggacctc gccgagcaga ccggctacca ggatctaccg gccaccgacg cggacggctt
     1261 ggcggcctgg ttccgcaccg ccgcgcacag cgggtcgctc gtgcggtatc tggagccgtt
     1321 tgcgcacacc gtcggggtga tgcagacgcc ggaggcgctg taccgggtgg cctacgagtg
     1381 cgtcgaagac ctggccgccg acaacgtggt gtacgccgag ataaggttcg cccccgagct
     1441 gcacatcgac cggggtctct cgctggacgc ggtcgtcgac gctgtgctgg ccggcttcgc
     1501 cgacggtgag aaggccgccg gcagtgaggg tcgcacgatc acggtgcgct gcctggtgac
     1561 cgcgatgcgc caccaggccc ggtcgcagga gatcgcccag ctggcgatcc agttccggga
     1621 caagggcgtt gtgggtttcg acatagcggg cgcggaggcc ggttacccgc cgacgcgtca
     1681 cctcgacgcc ttcgagtaca tgcggaacca caacggccac ttcacgattc acgccggaga
     1741 ggcgttcggg ttgccgtcca ttcatgaggc cattgcgttc tgcggtgctg accggctcgg
     1801 ccacggcgtc cgcatcgccg acgacatcac cgtggcgccg gacggcactg tcgacctcgg
     1861 ccggctggca tcgctgttgc gcgacaagcg gataccgctc gagctgtgcc cgagctccaa
     1921 tgtgcagacg ggcgccgtcg gcagcattgc cgagcatccc ttcgaccttc tcgcaaaatg
     1981 ccgtttccgt gtcaccgtca acaccgacaa ccgactgatg agcgacacca cgatgagtca
     2041 ggagatgctg cgcctggtgg aggccttcgg ctacggctgg agcgatctgg cgcggttcac
     2101 catcaacgcg atgaaatcgg cgttcatccc gttcgaccag cggct
//
LOCUS       DASYLX010000770         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3662266,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000770 DASYLX010000000
VERSION     DASYLX010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662266"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1569)
                     /locus_tag="VGP27_18050"
     CDS             complement(166..1569)
                     /locus_tag="VGP27_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7853233.1"
                     /translation="MGRRLGRDALDLTIDAALQAIADARLTVAEIDGLATYPGGVSGF
                     GGFSGPGSPEVHDALRLSLRWHSGGAEGAGQLQAMINAMMAVSIGLARHVLVYRTVTE
                     STAQGTGRRSGIGVGGSDIDGLATYPGGVSGYGGFSGPGSPEVHDALRLSLRWHSGGA
                     EGAGQLQAVINAMMAVSIGLARHVLVYRTVTESTAQGTGRRAGIGVGSSQIDGPQQWL
                     IPFRAYSAANWLALYAQRHFHEFGTTQEQMAQIPLNGRRNAALNPRAVFRTPMTLDDY
                     LGSRMVSSPFRLYDCDVPVDGSTALVVSVVEHAACVDHPVAQVEAVGTALRGRPSWEQ
                     WQDLTTMPARTSAEHMWSRTELRPGDVDVAELYDGFSWLTMSWLEALGFCGHGESGPF
                     IEGGTRIALDGELPLNTNGGQLSGGRLHGYGYLHEAVLQLRGEAGARQVAGRPEVAVV
                     SNGGGPISGCMLLTRLA"
     gene            complement(1616..1732)
                     /locus_tag="VGP27_18055"
     CDS             complement(1616..1732)
                     /locus_tag="VGP27_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7853234.1"
                     /translation="MTNIVDVAPDDMRIGMPVEVVFEDHPPVYLPVFRPIAS"
BASE COUNT          305 a          619 c          559 g          268 t
ORIGIN      
        1 gaggcggacg ccgtaagcct gttcgccctg gccagcaccg ccgccacatc gccgcgcggt
       61 tatccccgcg accgaaacag ggcgcgagcg cacgtattac ggttcgcgtg gccggcgttg
      121 gccatctcag gcgacccagc aagcagtgct caatcgagtc agcggtcacg ccagcctcgt
      181 aaggagcatg catcctgaaa tagggccgcc gccattggat accacggcaa cctccggtcg
      241 tcccgcgacc tgtcgtgcgc ctgcttctcc ccgcagctgc aagaccgcct cgtggagata
      301 gccgtatccg tgcagccggc cacctgagag ctgacccccg ttggtgttca gcggcagttc
      361 gccgtcaagc gcgatgcggg tgccgccctc gatgaagggc ccgctctcgc cgtgcccaca
      421 gaagccgaga gcctccaacc acgacatcgt cagccagctg aatccgtcgt acaactctgc
      481 cacgtcgaca tcgccgggcc gcagctcggt tcgggaccac atatgctccg cggaggtgcg
      541 cgccggcatc gtcgtcaagt cttgccattg ctcccaggac gggcgtccgc gaagggcggt
      601 cccgacggct tcgacctgcg caaccggatg gtccacgcag gcagcgtgtt cgaccaccga
      661 cacgaccagc gcggtggagc cgtcgacggg aacgtcgcag tcgtacaggc gaaacggcga
      721 ggacaccatc ctcgagccca gatagtcatc gagggtcatc ggtgtgcgaa acaccgccct
      781 agggttgagc gcggcattgc gacggccatt cagcgggatc tgcgccatct gctcttgggt
      841 tgttccgaac tcatggaagt gccgctgggc gtacagcgcc agccagttgg ccgcagaata
      901 agcgcggaac gggatcaacc attgctgtgg cccgtcgatt tggctgctac ccacgccgat
      961 gccggcacgc cgccccgtgc cttgtgcggt ggactcggtg acggtgcgat acaccaggac
     1021 atggcgcgcc agcccgatcg acacggccat catcgcgttg atcaccgcct gcagctgtcc
     1081 cgcaccctcc gcgccgccgc tgtgccagcg aagcgacaag cgcaacgcgt cgtgcacttc
     1141 gggtgagccg ggaccggaga aaccgccgta tccgctgacc cctcccgggt aggtcgccaa
     1201 cccgtcgata tcgctgcccc ccacgccgat gccggaacgc cgccccgtgc cctgtgcggt
     1261 ggactcggtg acggtgcggt acaccaggac atggcgcgcc agcccgatcg acacggccat
     1321 catcgcgttg atcatcgcct gcagctgtcc agcaccctcc gcgccgccgc tgtgccagcg
     1381 cagcgacaag cgcaacgcgt cgtgcacttc gggtgagccg ggaccggaga aaccgccgaa
     1441 tccgctgacc cctcccgggt aggtcgccag cccgtcgatt tcggccacgg tcagccgcgc
     1501 atcggcgatg gcctgaagcg cggcgtcgat ggtgagatcc agggcgtcac ggccgagccg
     1561 ccgtcccacc tgcgactgac cgatgcggag atgacggctc ggcgctcggc tccggtcacg
     1621 aggcgatcgg ccggaacacc ggaagataga ccggcgggtg gtcctcgaag acgacctcga
     1681 ccggcattcc aatacgcatg tcgtcgggtg cgacgtcgac gatgttggtc atcagccgaa
     1741 gaccgggctg c
//
LOCUS       DASYLX010000771         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3444502,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000771 DASYLX010000000
VERSION     DASYLX010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3444502"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VGP27_18060"
     CDS             <1..447
                     /locus_tag="VGP27_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV7853235.1"
                     /translation="LARATHTQDASYLDVIHSGEGEWNPSDYAYHLTRRARGLPLWFS
                     LAVHGIEAYRAAIEVAVTLAHETAELIKAAPQLELIREPDLGVVLFRRPGWKPEDYDA
                     WAQRLHEDQVAFIPPTKWEGETVGRFAFLHPHTHLELVREVLARTE"
     gene            complement(452..1318)
                     /locus_tag="VGP27_18065"
     CDS             complement(452..1318)
                     /locus_tag="VGP27_18065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7853236.1"
                     /translation="MDLSGVGIWSHQLRYGDKAQSADAAAELEELGYSALWIPDVGGR
                     TPVLASVEHLLSATNDVVIATGILNLWMNDPADVAASYASLTAAHGERFLMGIGVSHA
                     PLIDSTEPGRYRKPLAATASFLDALDSATPPVPVEARVLAALGPKMLRLAADRTRGAH
                     PYLVTPQHTRRAREVLGEGPLLLPEQTVILTADAAEARAIGTRWIKNYLAMPNYRNNL
                     LRLGFSSDDVASVSGRLLDAIIAWGDEGAVIGRVNEHLSAGADHVCVQVLTANPNEYP
                     REEWRRIAAALG"
     gene            complement(1345..1737)
                     /locus_tag="VGP27_18070"
     CDS             complement(1345..1737)
                     /locus_tag="VGP27_18070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7853237.1"
                     /translation="MTAPKSLRELFDQIGLREVPSTDGTVVMEMPVDERVTNTAGGLQ
                     GGLIATMADVTAGQLAARSTPFGHAVATTDLFIRYLRPIKERNGPARAVARILRTGKR
                     SVVIQVDIYRGDDGELGATATVNFAAVG"
     gene            1775..2500
                     /locus_tag="VGP27_18075"
     CDS             1775..2500
                     /locus_tag="VGP27_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEV7853238.1"
                     /translation="MTLADRLESQLACVLPGDQLFRCLGVDEAAWQRFSSHWDDLKPD
                     SYAAECGTTRLRRYGHFIFTPADRRFRLLPHDTFAQPEDSNPLYLDRDRDFEPLTASF
                     IDDALLRGLLRLLGDVATALDDVADWSAKVTPFRVLASAGHVGNPTPEGLHRDGVTLV
                     TSLLIGRDNALGGESSVDDFAGNRLLATVLSEPGTMLLGDDRNTLHCVTPIRPLDPAR
                     PARRDVLVITFASHQRSDRRAPR"
     gene            2557..>2940
                     /locus_tag="VGP27_18080"
     CDS             2557..>2940
                     /locus_tag="VGP27_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_6728 family protein"
                     /protein_id="HEV7853239.1"
                     /translation="MQTFLPCPGFADSARVLDKRRLGKQRVETIQVLRALTVAGYGWR
                     HHPAAAMWAGYEEALVRYGLEMCAVWCESGHADTCAATLVEDLAQAVGQRSIRSQAEL
                     AAAGELPPWLGDPAFHLSHQSALVQK"
BASE COUNT          456 a         1004 c          980 g          500 t
ORIGIN      
        1 ctcgctcgcg cgacgcacac tcaggacgcg tcgtatctcg acgtcatcca cagcggggag
       61 ggcgagtgga acccgtccga ttacgcctac cacctgaccc gccgcgcgcg tggcctgccg
      121 ttgtggttct ccctcgcggt gcacggcatc gaggcctacc gcgccgccat cgaggtcgcc
      181 gtcaccctgg cgcacgagac agccgaactg atcaaggccg caccgcagct ggagttgatc
      241 cgggagcccg acctcggcgt ggtcttgttc cgcaggcccg gctggaaacc cgaggactac
      301 gatgcgtggg cgcagcggct gcatgaggac caggtcgcgt tcatcccgcc gacgaagtgg
      361 gagggagaaa ccgtcggccg gttcgcgttc ctgcatcccc acactcacct tgagctggtt
      421 cgcgaggtgc tggccagaac ggagtgagcg tctaaccaag cgcggcggcg atacgccgcc
      481 actcctcgcg cggatactcg ttcgggttgg cagtgagcac ctgcacgcag acgtggtcgg
      541 cgcccgcgga cagatgctcg ttcacccggc cgatcacggc gccttcgtcg ccccacgcga
      601 tgatcgcgtc gagcaacctg ccgctgaccg acgcgacgtc atcggaggag aagccgaggc
      661 gcagcaagtt gttcctgtag ttgggcatcg ccagatagtt cttgatccaa cgggttccga
      721 tggcacgggc ctcggcggcg tcggctgtca ggatgactgt ctgttcgggc agcagcaggg
      781 ggccctcacc cagcacctcg cgcgcccgac gcgtgtgctg gggcgtgacg aggtacgggt
      841 gggcgccgcg ggtccggtcc gccgcaagtc tcagcatctt cggccccagc gccgcgagca
      901 ccctggcttc gaccggtacg ggtggcgtgg cgctgtcgag cgcgtcgagg aacgacgcgg
      961 tcgcggccag cggcttgcga taccggccgg gctcggtcga gtcgatgagt ggcgcgtggc
     1021 tgacgccgat gcccatcagg aaccgctcgc cgtgagcggc ggtcagtgac gcgtaggacg
     1081 ccgccacgtc ggcggggtcg ttcatccaga ggttgaggat tcccgtcgcg atcacgacgt
     1141 cgttggtcgc ggagagcaga tgctccaccg acgccagcac cggtgtccgg ccgccgacgt
     1201 cgggtatcca aagcgccgag taacctaact cttccaattc cgctgcggcg tcggcggatt
     1261 gcgctttgtc gccgtatcgc agctgatgac tccagatgcc aacgcccgaa agatccatgt
     1321 tgtctcctat tccggctatc cgaactatcc gacggccgcg aagttgacgg ttgccgtcgc
     1381 accgagttcg ccgtcgtcac cgcggtatat gtcgacctgg atgacgacgg accgcttgcc
     1441 ggtacgcaaa atccgggcga cggctcgcgc cggaccgttg cgctccttga tcggccgcag
     1501 atagcggatg aagagatccg tggtggcgac ggcgtggccg aacggcgtgc tgcgggcggc
     1561 cagttgtccg gccgtcacgt cggccatcgt ggcgatcagc ccgccttgca ggccaccggc
     1621 ggtgttcgtc acccgctcgt cgacgggcat ctccatgacg acggtgccgt cggtcgacgg
     1681 cacctcccgc agcccgatct ggtcgaacag ctcccgcagg gatttcggcg ccgtcacggg
     1741 gaatgacctt acggcaggcg ggtactcgcg cctgatgacc ctggctgacc ggcttgagtc
     1801 gcagttggcg tgcgtgctgc ccggggacca actgtttcgt tgtctcggtg tggacgaggc
     1861 ggcgtggcaa cggttctctt cgcactggga cgacctgaag cccgacagct atgcggccga
     1921 gtgcggcacg acgcgccttc ggcgatacgg ccacttcatc ttcacgcctg ccgatcgccg
     1981 cttccggctt ctgccgcacg acacgttcgc ccaacccgaa gattccaacc cgctctacct
     2041 cgaccgagat cgcgacttcg aaccgctcac cgcgagcttc atcgacgacg cgttgctgcg
     2101 cggcctgctg aggctgctgg gtgacgtggc gaccgctctc gacgacgtcg ccgactggag
     2161 cgccaaggtg acgccgttcc gagtcctcgc gtccgccggc cacgtaggca acccgactcc
     2221 agaagggctg catcgcgacg gcgtcaccct cgtcacctcc ctgctgatcg gaagggacaa
     2281 cgcgctggga ggcgagagca gcgtcgacga tttcgccggc aatcgactgc tggccaccgt
     2341 gctgagcgaa cccggcacca tgctgctggg cgacgaccga aacacattgc actgcgtgac
     2401 gccgatccga cccctggacc cggcccgtcc cgcacgtcgg gacgtcctcg tcatcacttt
     2461 cgcttcgcac cagcgctcgg atcgtcgcgc accccgatga gcttgttgcc atcgtggctg
     2521 tgtgcctgcc tcggtcgccg ggtacccggc cctgcgatgc agaccttcct gccgtgccct
     2581 ggattcgcgg actccgcgcg ggtgctcgac aagcggcgtc tcggcaagca gcgcgtcgag
     2641 acgatccaag tgctgcgtgc gctgaccgtt gcggggtacg gatggcggca tcatcccgcc
     2701 gccgcgatgt gggccggcta cgaggaggcg ctggttcgat acgggctcga gatgtgtgcc
     2761 gtctggtgcg aaagcggtca cgcagacacc tgtgccgcaa ccctcgtcga ggatctggcg
     2821 caagcggtcg ggcagcggtc gatacgcagc caggccgaac tcgcggccgc cggtgagctc
     2881 ccaccctggc tcggtgaccc cgcgtttcac ctcagccacc agtcggcatt ggtgcagaag
//
LOCUS       DASYLX010000772         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1579730,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000772 DASYLX010000000
VERSION     DASYLX010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579730"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1120
                     /locus_tag="VGP27_18085"
     CDS             164..1120
                     /locus_tag="VGP27_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7853240.1"
                     /translation="MHERDVSVVLEAFLDSSLDEVLTWLSTEVPEVTAVEVGAGGYAP
                     HPHLDPDALLSDASARQAWLDKLVANGIRLDALNVWGNPLHPDKSIADNHDAALRRAV
                     RLAGELGTDTIVAMSGAAAGAMGDHAAVFGAGGWLPYLEGVHDRQWEESVVPYWSQLS
                     EFAAKENPDVKICIELHPGTTVYNVETFEKFITLGENLFANLDPSHFFWMQMDADRVV
                     ERIMPRIGHVHAKDVTFNDDALALNGLLDHRWPSSPEKMPWNFSVPGHGKDAQWWHQF
                     VARFDGSAVRAFSIEHEDPFVDSKTGVKESAALLKSALAESH"
     gene            1157..2281
                     /locus_tag="VGP27_18090"
     CDS             1157..2281
                     /locus_tag="VGP27_18090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7853241.1"
                     /translation="MTERLKVGVVGAGVIAQVMHLNYLRELSDRFDVVALCDISEENV
                     NNNADRYDVGKRYTDWQDMLDDADIDAVLILTSGSHAPIAIAAANAGKHILVEKPMCF
                     SVAEGLEMKSAADAAGVALMVAYPKRYDPAYERFRDIAVNVAEPRLMRVTTFESPFLP
                     YIGHYALAPVAPIPNDAIETLTAATQASISRAIGEANPFLREEYHLVLLDTLVHELNT
                     VRGVLGEPTSLEYVDMQPGQLTVILKFGDLSVPINWMDLPGITRYGMEFALYGPQQRV
                     TLSFPSPFLRSAPATVEIIEGEPGTTKSRSISEITSYDSAFKLELEAFHRCVTTGEAP
                     ATGAEDAIHDLALCEAIIRFVQDPRPISDPSAIRAVASAN"
BASE COUNT          491 a          768 c          730 g          422 t
ORIGIN      
        1 tacaaccagc tcaagttcgg tatcacgccg gaggtcaacg cgttggcgac gctgatgctg
       61 gtcggcacgt tggccgtcgc aggcatgacc ttcggggcgc tgcggctggt gaaggtactc
      121 gcatcggtca ccaagaccac caaacgatcg gaagaacccg ctagtgcatg agcgtgacgt
      181 ttctgtagtg ctcgaggctt ttctggactc ttcgctcgac gaagtgctga cctggttgag
      241 caccgaggtg cccgaagtca ccgccgtcga ggtcggcgca ggaggctacg caccgcaccc
      301 ccacctggac cccgatgcac tgctgagcga cgcgtcggca agacaggcgt ggctggacaa
      361 gctcgtcgcc aatggaatcc ggctcgacgc attgaacgtg tggggcaacc cattacaccc
      421 cgacaagtcg atcgccgata atcacgacgc tgcgttgcgc cgcgctgttc ggttggccgg
      481 tgaactcggc accgacacga tcgtggcgat gtcgggtgcc gccgccggcg ccatgggtga
      541 ccatgccgct gtgttcggcg ccggaggctg gctgccgtat ctggagggag tgcacgaccg
      601 ccaatgggag gagtccgtcg tgccgtactg gtcgcaactc tccgagttcg cggccaagga
      661 gaaccccgac gtcaagatct gtatcgagct gcaccccggg accaccgtct acaacgtcga
      721 gaccttcgag aagttcatca ccctgggcga gaaccttttc gccaacctcg accccagcca
      781 cttcttctgg atgcagatgg acgccgaccg ggtggttgag cgaatcatgc cgcgaatcgg
      841 tcacgtacac gccaaggacg tgacgttcaa cgacgatgcg ctggccctca acggcctcct
      901 cgaccacaga tggccgtcaa gcccggagaa gatgccgtgg aacttctcgg ttcccggtca
      961 cggcaaggat gcccagtggt ggcatcagtt cgtcgcccgc ttcgacggct cggcggtccg
     1021 cgcgttctcc atcgaacacg aggacccgtt tgtggactcg aagaccgggg tcaaggagag
     1081 cgccgctctt ctcaagtcgg cgctggccga atcccactga gccaatggcg cacacgaaat
     1141 acaggaggca tagccaatga ccgagcgact caaagtcggt gtcgtcggtg ccggagtgat
     1201 cgcgcaggtc atgcacctga actacttgcg cgaactgtcc gaccgcttcg acgtcgtcgc
     1261 actgtgcgac atttcggagg aaaacgtcaa caacaacgcc gaccgatacg acgtcggcaa
     1321 gcggtatacc gactggcagg acatgctcga cgacgctgac atcgatgccg tcttgatcct
     1381 gacttcaggt agccacgcgc cgatcgcgat cgcggcggcg aatgcgggta agcacatcct
     1441 ggtcgaaaag ccgatgtgtt tctcggtcgc cgaaggtctg gaaatgaagt ccgccgccga
     1501 cgcggcgggt gtggcgctga tggtggctta tcccaagcgt tacgacccgg catacgagcg
     1561 tttccgcgac attgccgtga atgtcgccga gccgcggctc atgcgggtca ccacattcga
     1621 gtcgcccttt ctcccgtaca tcggccatta cgcgctggcg cccgtcgccc cgatccccaa
     1681 cgatgcgata gagaccctaa cggcggcaac acaggccagc atcagccggg ccatcggcga
     1741 ggcgaatccg ttcctgcgcg aggagtatca cctggtgctg ctggacacac tcgtccacga
     1801 gctgaacaca gtgcgcggcg tgctcggtga gccgaccagc cttgaatacg tcgatatgca
     1861 acccggccag ctgaccgtca tcctcaagtt cggcgacctg tcggtaccga tcaattggat
     1921 ggatctacct ggaatcaccc gctacggaat ggaattcgca ctctacggcc cgcagcagcg
     1981 ggtcaccttg agcttcccgt cgccgtttct gcgcagcgca ccggcgaccg tcgagatcat
     2041 cgagggcgaa ccgggcacca ccaaatcgcg cagcatcagc gaaatcacct cgtatgacag
     2101 cgcgttcaag ctcgaactgg aagccttcca tcgctgtgtc acgacagggg aggcgccggc
     2161 aacgggtgca gaagacgcaa ttcacgacct cgcactgtgc gaggccatta tccggttcgt
     2221 acaagacccg cggccgatct ccgatccgag cgcgatccgc gccgtggcat ccgccaacta
     2281 gagagaatgc gaaacatgag caacgacagc gaaatcatcg tcgcgaacgc gcctgtcagc
     2341 tacggcgcct tcgaggtgac gatcggattc gatccgaacg ttcctgacgg agacgacatc
     2401 ctggaccagg t
//
LOCUS       DASYLX010000773         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1358236,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000773 DASYLX010000000
VERSION     DASYLX010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358236"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGP27_18095"
     CDS             complement(<1..567)
                     /locus_tag="VGP27_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853242.1"
                     /translation="MKRNRTLLNVSIFTVAMLLVAAMLVVVFGEFRFASESKYHATFT
                     DAARLKAGQDVRISGVPVGTVGSLKLNPDNTVDVAFQLDKKYQLYTSTRAVVRYENLV
                     GDRYLEITAGPGELRKIAPGSTIPKENTAPALDLDALLGGLRPVLKGLDGGKINEVSS
                     AVMELLQGQGGALSNLLSTTSAFTQNLAA"
     gene            complement(567..1769)
                     /locus_tag="VGP27_18100"
     CDS             complement(567..1769)
                     /locus_tag="VGP27_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853243.1"
                     /translation="MSDGGAKRSHVRIAAAILAALVAAAVVFTYLAYTAAFTSTDNVT
                     VTSPRAGLVMDRDAKVKYRGIQIGEVKAIDYAGDQAKLTLAIDSNEMRFIPSNAVVRI
                     ASTTVFGAKSVEFVPPEQPSGSPLRPGSQVQASSVQLEVNTLFQTLTDVLHKIDPVQL
                     NGTLSALAEGLRGHGDDLGALLTGLNYYLQQLNPKLPTLQQDFQKTAAVGNIYGDAGP
                     DLTRILDNVPAISNTIVDEQDNLNATLLAATGLANNGTDTLEPAQNDYIAAVNRLRAP
                     LKVAGDYSPEFGCILEGTGSVVDFFAPIIGGTRPGLFVSSNFLPGSPAYTYPESLPIV
                     NGSGGPNCRGLPHVPSKQLGGSWYHTPFLVTDNAYVPYQPNTELQFDAPSTAQFLFNG
                     AYAERDDY"
     gene            complement(1778..2575)
                     /locus_tag="VGP27_18105"
     CDS             complement(1778..2575)
                     /locus_tag="VGP27_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853244.1"
                     /translation="MPKVVDNIGEQALFYGETIRYIPNAVTRYRKETIRNIAEMTMGT
                     GALVMIGGTVGVAAFLTLASGGVIAVQGYSSLGNIGIEALTGFLSAFLNVRIVAPVIA
                     GIALAATIGAGTTAQLGAMRVAEEIDAVESMAVHSVSYLVSTRLMAGMVAIIPLYSLS
                     VLAAFFAARFTTVFINKQSAGLYDHYFNTFLVPTDLLWSFLQAIVMAIAVMLVHTYYG
                     YNASGGPVGVGIAVGQAVRTSLIVVVTITLFISLAVYGASGNFNLSG"
BASE COUNT          469 a          805 c          866 g          453 t
ORIGIN      
        1 cgccgcaagg ttctgggtga aggcgctggt ggtcgacaac aggttggaca gcgcgccgcc
       61 ctgaccctgg agcaactcca tgacggcgct gctgacctcg ttgatcttgc cgccgtcgag
      121 gcccttgagg accggacgta gcccgcccag aagtgcatcg aggtcgagtg cgggcgcagt
      181 gttctccttc ggaatggtgc tccccggcgc aatctttcgt aattcccccg gccccgcggt
      241 gatctcgaga taccggtcac cgacgaggtt ctcgtagcgg accaccgcgc gcgtcgacgt
      301 atagagctga tatttcttgt ccaactggaa ggcgacgtcg acggtgttgt cgggattcag
      361 cttgagcgaa ccgaccgttc ccaccggaac gccggagatg cgcacgtcct gtcccgcttt
      421 cagccgcgcg gcgtcggtga acgtcgcgtg gtatttgctt tcggacgcga agcggaactc
      481 gccgaacacc accaccagca tcgccgccac cagcagcatc gccaccgtga agatgctgac
      541 gttgagtagg gtccgattac gcttcatcag taatcgtccc tctccgcgta cgcgccgttg
      601 aacaagaact gcgccgtcga cggcgcgtcg aactgcaact cggtgttcgg ctggtatggg
      661 acatacgcgt tgtcggtgac gaggaacggc gtgtggtacc aggacccgcc caactgcttg
      721 ctcgggacat ggggcaaacc acggcaattg gggccgccgg acccattcac gatcggcagg
      781 ctctccggat acgtgtaggc cggcgaaccg ggcaggaagt tggaagacac gaacaggccc
      841 ggccgggtgc cgccgatgat gggcgcgaag aagtcgacaa cagagccggt gccttcgagg
      901 atgcatccga actcgggcga gtagtcaccg gccaccttca gcggcgcgcg cagccggttc
      961 accgcggcaa tgtagtcgtt ctgcgcgggc tccaacgtgt cggtgccgtt gttggccagg
     1021 ccggttgccg ccagcagcgt cgcgttgagg ttgtcttgtt cgtcgacgat ggtgttgctg
     1081 atggcgggca cgttgtcgag gatgcgcgtc aggtccggac cggcgtcgcc gtagatgttc
     1141 cccaccgcag ctgtcttctg gaagtcctgc tgcagcgtcg ggagcttcgg attcagttgc
     1201 tgcagataat agttcaggcc ggtaagcaaa gcgccgaggt cgtcgccgtg accgcgtaga
     1261 ccctccgcga gcgcgctcag cgtcccgttc agctgcaccg ggtcgatctt gtgcagcacg
     1321 tcggtcaacg tctggaagag cgtgttgacc tcgagttgca ccgacgacgc ctggacctgc
     1381 gacccggggc gtagcgggga tcccgaaggt tgttccggcg ggacgaactc caccgacttc
     1441 gcgccgaaaa ccgttgtgct ggcgatacgg acgaccgcgt tcgacgggat gaaccgcatt
     1501 tcgttgctgt cgatcgcaag cgtcaacttg gcctgatcgc ccgcatagtc gatcgccttg
     1561 acctcgccga tctggatacc gcggtacttc accttggcgt cgcggtccat gacaagacct
     1621 gctcgaggcg aggtgacggt gacgttgtcg gtcgacgtga atgcggccgt gtacgccagg
     1681 taggtgaaga cgacggcggc ggcgaccagc gccgcgagga tcgcggcggc gatccgcacg
     1741 tggctgcgct tggcccctcc gtcggacatc gccccaccta tccggaaagg ttgaaattgc
     1801 cggacgcgcc gtagacggcg agtgagatga acagggtgat ggtgacgacc acgatcagcg
     1861 aggtgcggac ggcctggccc accgcgatcc cgacgccgac cggaccgccc gacgcgttgt
     1921 acccgtaata ggtgtgcacc agcatcacgg ctatcgccat gacgatcgcc tgcaggaatg
     1981 accacaacag atcggttggt accaggaacg tgttgaagta gtggtcgtag aggcccgccg
     2041 actgcttgtt gatgaagacc gtggtgaagc gggcggcgaa gaacgcggcg agcaccgaca
     2101 gcgagtacag cgggatgatc gccaccatcc cggccatcaa ccgggtggac acgaggtatg
     2161 acaccgaatg cacggccatc gattcgaccg cgtcgatctc ctcggcgacc cgcatggcac
     2221 ccagttgcgc cgtggtaccg gcgccgatgg tggccgccag cgcgatgccg gcgatcacag
     2281 gcgcgacgat gcgcacgttg aggaacgcgg acaggaagcc ggtcaacgcc tcgatgccga
     2341 tgttgcccag cgacgagtag ccctgaacgg cgatgacacc gccggaggcc agggtcagga
     2401 acgccgccac accgacggtg ccaccgatca tcaccagcgc gccggtgccc atcgtcatct
     2461 cggcgatgtt gcggatggtc tccttgcggt agcgggtgac cgcgttgggg atgtagcgga
     2521 tcgtctcgcc gtagaacagc gcctgctcgc cgatgttgtc gacgaccttc ggcacgccgc
     2581 ggaaggcccg gcg
//
LOCUS       DASYLX010000774         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3004213,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000774 DASYLX010000000
VERSION     DASYLX010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004213"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="trpS"
                     /locus_tag="VGP27_18110"
     CDS             complement(<1..561)
                     /gene="trpS"
                     /locus_tag="VGP27_18110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV7853245.1"
                     /translation="MSKPADRVVFSGVQPTSDSLHLGNALGAVAQWAGLQDEFDAYFC
                     VVDLHSITLPQDPATLRRRTLATAAQYLAMGVDPFRATIFVQSHVPAHTELAWVLGCF
                     TGFGQASRMTQFKDKSQKEGSEATTVGLFTYPVLMAADVLLYDTDLVPVGEDQRQHLE
                     LARDVAQRFNSRFPDTFVIPEPMIPKA"
     gene            complement(587..982)
                     /locus_tag="VGP27_18115"
     CDS             complement(587..982)
                     /locus_tag="VGP27_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853246.1"
                     /translation="MLAPLLRELVAAEEPVLEAHGLTMWGYVVLLALDRSSMRTQAAL
                     AAAIGADKTRIIRTLDDLQDDGYIERRPDPEDRRVRLLAITDTGRRVKDAVQDEIQRG
                     EERWLGELGAEERRTFLRVLERMTPREWA"
     gene            1079..>1639
                     /locus_tag="VGP27_18120"
     CDS             1079..>1639
                     /locus_tag="VGP27_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEV7853247.1"
                     /translation="MPTKATNISQLHRIAVLKSIDIVDSVRLSDLDRTTPCEGWNLAD
                     LLAHMTVQHRGFAAAARGLGADHEVWHVDSVVGAVTADPQRAYSEAAHDVLEAFEDDG
                     TADAPFALPEFGEGAVFPGEMAMEFHFVDYVVHGWDVAASLGVPFELPADVVEAVLPL
                     VRAVPDGDFRTMAGAPFAPAVEQVGTD"
BASE COUNT          271 a          538 c          540 g          290 t
ORIGIN      
        1 ggccttcgga atcatcggct ccgggatgac gaaggtgtcg gggaatcgcg agttgaatcg
       61 ctgcgcgacg tcgcgcgcga gttcgaggtg ctgacgctgg tcctcaccga ccggtaccag
      121 gtcggtgtcg tagagcaaca catcggcggc catcagcacc ggataggtga acagccccac
      181 ggtggtggcc tcactgccct ccttctgcga cttgtccttg aactgcgtca tccgagaagc
      241 ctggccgaag ccggtgaaac agcccagcac ccaagcgagt tcggtgtgcg cgggcacgtg
      301 actctgcacg aagatggtcg cccggaaggg gtccacgccc atcgcgaggt actgcgctgc
      361 ggtcgcgagc gtgcggcgcc gaagggtggc cggatcctgc ggcaacgtga tggaatgcag
      421 gtcgacgacg cagaagtagg cgtcgaactc atcctgcagg cctgcccact gcgcgaccgc
      481 ccccagcgcg ttgccgaggt gcagggagtc ggaagtgggt tgcacgccgg agaacacgac
      541 gcggtcggct ggcttgctca tgatgggctc aatctttcca cccgcatcaa gcccattcgc
      601 gtggggtcat ccgctcgagg acacgcagga acgtccgccg ctcctcggcg ccgagttcgc
      661 cgagccaccg ctcttcgccg cgctggatct cgtcttgcac tgcgtctttg acacggcgcc
      721 ccgtgtcggt gatggccagt aaccgcaccc tgcggtcttc cgggtcgggt cggcgttcga
      781 tatagccgtc gtcctggagg tcgtcgaggg tgcggatgat ccgcgttttg tcggcgccga
      841 tcgccgcggc gagggcggcc tgggtccgca tcgatgacct gtccagagcc aacaggacga
      901 catagcccca catcgtcagg ccgtgcgctt cgagaaccgg ttcctccgcg gcgacgagct
      961 cacgcagaag cggcgccagc atcgcggcca ggtcggggcg ccgcggattc gacgacttcg
     1021 ctgccacccc gtcatgatag gcattgcact atcgtatgca tccgcttact atctacatat
     1081 gcctactaaa gccaccaata tctcccaact acaccgaatc gccgtgctga agtcgatcga
     1141 catcgtcgac tccgtccggc tgtccgatct cgatcggacg acgccctgcg aaggctggaa
     1201 tctcgccgat ctcttggcac acatgaccgt ccagcatcgc gggttcgcag ccgctgcaag
     1261 gggtttgggc gcagatcacg aagtctggca tgtcgattcg gtggtcggcg cggtgaccgc
     1321 cgacccgcag cgggcctact ccgaggcggc gcacgacgtg ctcgaagcat tcgaggacga
     1381 cgggacggcc gacgcgccgt tcgcattgcc cgagttcggc gagggcgcgg tgttccccgg
     1441 tgagatggcg atggaattcc acttcgtcga ctacgtcgtg cacggctggg acgtcgccgc
     1501 gtcgctgggt gtccccttcg aactacccgc cgacgtcgtc gaggccgtgc tgccgttggt
     1561 ccgtgcagtg cccgacggcg acttccgaac catggccggg gcgccgttcg cgccagccgt
     1621 cgaacaggtc ggcacggac
//
LOCUS       DASYLX010000775         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1907484,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000775 DASYLX010000000
VERSION     DASYLX010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907484"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="VGP27_18125"
     CDS             complement(<1..1943)
                     /locus_tag="VGP27_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV7853248.1"
                     /translation="MVPQHATPADESASAVQLNETAPVPYLSSSLRNPFPPIADYAFL
                     SDCENSCLISSTGSVEWLCVPRPDSPSVFGAILDRGAGHFRLSPYGVSVPSGRRYLPG
                     SLILETTWQTHTGWIIVRDTMVMGAWRDIETRSRTHRRVPMDWDAEHTILRTVRCVSG
                     TVELVMDCEPSFDYHRAGANWEYSASGYGEAVARANHNPDANPTLRLTSNLRIGLEGR
                     QARARTRLKEGDNVFVALSFSKHRAPQTYDEAVEKMWTTSECWRQWINIGNFPDHPWR
                     LYLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWIRDSTFALWGLYT
                     LGLDREADDFFSFIVDVSGANGHERQELQVMYGVGGERTLVEQELNHLSGYDNARPVR
                     IGNAAYNQRQHDVWGTMLDSVYLHARSRERIPEELWQVLKQQVEEAIKHWREPDHGIW
                     EVRGEPQHFTSSKIMCWVALDRGAKLAELHGWTKDTKEWHAIAEEIKADILANGVDAR
                     GVLTQRYGSDALDASLLLAVLTRFLPPDDPRIRATVLAIADELTDQGLVLRYRVEETD
                     DGLSGQEGTFTTCSFWLVSALVEIGEINRAKRLCERLLSFASPLHLYAEELEPRTGRH
                     LGNFPQAFTHLALINAVVHVIRA"
     gene            complement(2018..2509)
                     /locus_tag="VGP27_18130"
     CDS             complement(2018..2509)
                     /locus_tag="VGP27_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV7853249.1"
                     /translation="MTATDEYLKNNEEYAKNFSGPLPLPPSKQVAVVACMDARLDVYR
                     ILGLNEGEAHVIRNAGGVVTDDEIRSLAISQRLLGTKEIILIHHTDCGMLTFTDDDFK
                     ASIQKDTGIKPEWAAEAFPDLDEDVRQSIRRIEASPFVTKHESLRGFLFDVATGRLNE
                     VKA"
     gene            complement(2609..>3842)
                     /locus_tag="VGP27_18135"
     CDS             complement(2609..>3842)
                     /locus_tag="VGP27_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEV7853250.1"
                     /translation="MIVSAGWYIALVELWPADSRPYIGGSTSNSLLQLALGYNGIDRI
                     AGGGFPGGGSGGDGPGGHGGPGGAGNIFFGGSPGIGRMFGQSMGTEVSWLLPAALIGT
                     VAALWLTRKAARTDQLRASLLLWGGWLLVTAAVFSFMDGVIHPYYTVALAPAVAALAG
                     ISVRELWRVRDRLWSRVVLATMLAATGVWAYFLLGRTPEWLPALRWVVLAGSVIVALM
                     LAVGAQRRGPQVALLAAGAILFGVAAPAAYAIETVATAHNGPVSTSGPSKGDAFGHPG
                     GSGGPREGGPGGPGGPGGQMAQNAALAELVGGLDNRWAAATIGSMGASSLELKTGASI
                     MAIGGFTGSDDSPTLAQFQDYVTDHDVRYFIAGERFGPPGHRDTGAAAQITAWVKKNF
                     RPTDVGGATVYDLTATVS"
BASE COUNT          666 a         1331 c         1198 g          647 t
ORIGIN      
        1 tcggcgcgga tgacgtgcac cacggcgttg atcagcgcca gatgggtgaa cgcctgcggg
       61 aagttgccga gatgccgacc ggtgcgcggc tcgagctcct cggcgtacag gtgcagcggg
      121 ctcgcaaagg acagcagacg ctcgcacaga cgtttcgctc gattgatctc accgatctcc
      181 accagcgccg acaccagcca gaacgagcag gtcgtgaagg tgccctcctg gccggacagg
      241 ccgtcgtcgg tctcctcgac gcggtaacga agcaccaagc cttggtcggt caactcgtcg
      301 gctatcgcca gcaccgtcgc gcggattcgt gggtcgtcgg gcggcagaaa ccgggtcagc
      361 accgccagca acagcgacgc gtcgagtgcg tcgctgccgt aacgctgggt gaggacgcca
      421 cgggcatcca caccgttggc aaggatgtcg gccttgatct cctcggcgat ggcgtgccac
      481 tccttggtgt ctttcgtcca gccatgcagc tcagccagct tcgccccacg gtccagcgcg
      541 acccagcaca tgatcttgct ggacgtgaaa tgctgcggct caccgcgcac ctcccagatc
      601 ccgtggtcgg gttcgcgcca gtgcttgatc gcctcttcca cttgttgctt cagcacctgc
      661 cacaactcct ccggaatccg ttcgcgggac ctggcgtgca gatacaccga atccaacatc
      721 gtgccccaaa cgtcatgctg ccgctggttg taggcagcgt tgccgatccg caccggacgc
      781 gcattgtcgt agccggacag gtgattcagc tcctgctcga cgagggtgcg ctcgccgcca
      841 acgccgtaca tcacttgaag ttcctggcgc tcatggccat tcgcgccgga cacgtcgacg
      901 atgaaggaga agaaatcgtc ggcctcgcgg tccaggccca acgtgtacaa gccccatagc
      961 gcgaaggtcg aatcacggat ccaggagtag cggtagtccc agttacgttc gccctgaggg
     1021 gtttccggca gcgaggtggt cggcgccgcc agcagcgcac cggtcggcga atacgtcaac
     1081 cccttcaacg tcaacgcgct gcgctgcaag tataaccgcc acgggtgatc cgggaagttg
     1141 ccgatgttga tccactgccg ccagcattcg ctggtcgtcc acatcttctc cacggcctcg
     1201 tcgtacgtct gcggcgcgcg atgcttcgaa aaactcagcg cgacgaagac gttgtcccct
     1261 tctttcagcc tcgtgcgggc ccgcgcctgg cggccctcca gcccgatgcg caggttggac
     1321 gtcagccgca gcgtcgggtt cgcgtccggg ttgtggttgg ctcgcgcaac cgcttccccg
     1381 tagccactgg ccgagtactc ccagttcgcg ccggcccggt ggtagtcgaa cgacggctcg
     1441 cagtccatca ccagttcgac ggtgccgctc acacaccgca cggtgcgcag aattgtgtgt
     1501 tcggcgtccc agtccatcgg tacgcggcga tgcgttcgcg accgcgtctc gatatcgcgc
     1561 cacgcgccca tcaccatcgt gtcgcggacg atgatccagc cggtgtgggt ctgccaggtg
     1621 gtctccagaa tcaggctgcc cggcaagtaa cgcctgccgg aagggacgga gacgccgtag
     1681 gggctcagcc ggaagtgtcc tgcgccgcga tccaggatgg ccccgaaaac gctgggcgaa
     1741 tcgggccgcg gcacgcatag ccattccacc gaaccggtcg acgagatcag gcagctgttc
     1801 tcgcagtcgg agaggaaggc gtaatcggcg atgggcggaa aggggttccg caaactgctc
     1861 gacaagtatg gcaccggggc ggtctcgttg agctgcacgg ccgaggcgct ttcgtcggcg
     1921 ggcgtcgcgt gctgcggaac catacggcca tcattgaccg ctgccccctc ccgcgtccgc
     1981 gtttagtcgc tccccgatgg cgtgggggcg acctgcctca cgccttcacc tcgttgagcc
     2041 ttccggtggc aacgtcgaaa aggaaaccgc gcaacgactc gtgcttggtg acaaacgggc
     2101 tcgcctcgat acgccggatc gactgtcgca cgtcttcgtc cagatcgggg aaggcctcgg
     2161 ccgcccattc gggcttgatg ccggtgtcct tttgaatgga tgccttgaag tcgtcgtcgg
     2221 tgaaggtcag catcccgcag tcggtgtggt ggatgaggat gatctcctta gtgccgagga
     2281 gccgctggct gatcgccagc gagcggatct cgtcgtcggt gaccactccc cctgcgttgc
     2341 ggatgacgtg cgcctcgccc tcgttcaagc cgagtattcg gtacacgtct agccgggcgt
     2401 ccatgcacgc caccaccgcc acctgcttgc tcggtggcag cggcagtggg cccgagaagt
     2461 tcttcgcgta ctcctcgttg ttcttcaagt attcgtcggt cgcggtcatt tcgacctcct
     2521 tgtcggctcg caaacgtcga agacgataac aacacggcgc aggtctgcca cgagtcagaa
     2581 tcacgccgat tcttcctcgc cgaacgcgct aggaaacggt ggcagtcagg tcgtagaccg
     2641 tggcgccgcc gacatcggtc ggcctgaagt tcttcttcac ccacgccgtg atctgagctg
     2701 cggcacccgt gtcgcggtgg ccgggcggcc cgaagcgttc acctgcgatg aagtaccgca
     2761 catcatggtc ggtgacgtag tcctggaact gcgccagcgt gggcgagtcg tcgctgccgg
     2821 tgaacccgcc gatcgccatg atcgacgcgc cggtcttcag ctcgagactg ctcgcaccca
     2881 tcgacccgat agtcgctgcg gcccaacgat tatcgaggcc acccaccagc tctgccaacg
     2941 cggcgttctg ggccatctgg ccgcccgggc cacccggacc gccgggtcca ccctcgcgtg
     3001 gtccgccaga accgcctggg tggccgaacg cgtcaccctt gctcggaccc gacgtcgaca
     3061 ccggcccgtt gtgcgcggtc gcgaccgtct cgatcgcgta ggccgccggc gccgccacgc
     3121 cgaacaggat cgcccccgcg gccaacagcg cgacctgggg gccccgccgc tgtgcgccca
     3181 ctgcgagcat caacgcgacg atcaccgatc cggccagaac gacccaccgc agcgcgggca
     3241 gccactccgg agtgcggccc agcaagaagt acgcccacac gcccgttgcc gccaacatcg
     3301 tcgccagcac gacgcgcgac cacaggcggt ctctgacccg ccacaattcg cgcaccgaga
     3361 ttcccgccag cgctgcgacc gccggggcca gagcgacggt gtagtacggg tgaatgacgc
     3421 cgtccatgaa gctgaagaca gcggcggtca ccagcagcca accaccccac agcagcaggc
     3481 tcgcgcggag ctgatccgtg cgtgcggcct tgcgggtcaa ccacagggcc gccaccgtgc
     3541 cgatcaatgc ggcgggaagc agccaggaca cctcggtgcc catcgactgc ccgaacatcc
     3601 tgccgatgcc aggcgagccg ccgaagaata tgttgcccgc cccgccggga ccgccgtgtc
     3661 caccgggacc gtcgccgccg gagccgccac ccgggaaacc accgcctgcg atgcgatcga
     3721 tcccgttgta gcccaacgcc aattgcagca ggctgttcga cgtggagccg ccgatgtacg
     3781 gccgggagtc agcgggccac aactccacaa gagcgatgta ccagcccgcg gacacaatca
     3841 tc
//
LOCUS       DASYLX010000776         9237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2565184,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000776 DASYLX010000000
VERSION     DASYLX010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565184"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGP27_18140"
     CDS             <1..451
                     /locus_tag="VGP27_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853251.1"
                     /translation="VDELAAELQRVLSKLFSVLRRGDVKGSTAGGDLTLAQLSILLTL
                     LEKGPIRMTDLAAHERVRTPTTTVAIRRLEKLGLVKRSRDASDLRAVLVEVTPRGLVQ
                     HREALAARRDALAAMLGKLTNDDVETLTKALGPMSRLAGQESGHEES"
     gene            complement(448..1788)
                     /locus_tag="VGP27_18145"
     CDS             complement(448..1788)
                     /locus_tag="VGP27_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HEV7853252.1"
                     /translation="MAPDPSFAPTQLAARAAYLLRGNDLGVMTTAAPLLYPHMWSWDA
                     AFVSIGLAPLSVERAVVELDTLLSAQWSNGMIPHIVFANGVDGYFPGPARWATSALAV
                     NAPRTRHTSGITQPPVHAIAVQRILDHARSRGRSTRAVAEAFLDRRWDGLVRWHRWLA
                     EARDQNSHGRVTLYHGWESGMDNSPRWDAAYANVIPGNVPEYQREDNTIVTDASQRPS
                     DLEYDRYLWLVEEMKSAHYDDDLLPKVMSFAVEDVFVSAVLSVACTVLAEIGEDYKRP
                     HADVRDLYGWAERFRAGVIETTDERTGAARDCDVRAGRWIATETVAQFAPLLCGGLPH
                     DKERRLLRLLEGPRFCGHPDLRYALIPSTSPVSRDFRSREYWRGPVWPVMTWLFSWAF
                     ARRGWAERSLILRREGLRQASDGTFAEYYEPFTGEPLGSMQQSWTAAAVLDWLG"
     gene            1823..2488
                     /locus_tag="VGP27_18150"
     CDS             1823..2488
                     /locus_tag="VGP27_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853253.1"
                     /translation="MPTAFITGPAGGLGTAIAEALAPSHTLLLGGRPSDRLDALAERL
                     GATTWPLDLEDTDAIPAVVEPIDELDVLVHNAGAAFPGRVAESTVDEWRVTMEVNVVG
                     AVALTLALLPALRAARGQVLFINSGAGIKASPGLASYSASKFALRGFADSLRNDEPQL
                     RVTSIHPGRIATVMQEGLVAYEGGEYKSERFLRPQTVANIVAATVNAPPEADIQEVIV
                     RPR"
     gene            2541..2957
                     /locus_tag="VGP27_18155"
     CDS             2541..2957
                     /locus_tag="VGP27_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV7853254.1"
                     /translation="MSKMRETAEQFFDACETGKGWDGCAPYCHPGATFAGQTDALAGI
                     DSLEAYTEWMKNLLTPLPDGTAEVRSFAVDEDRNNVAAYGIFRGTHTGEGGPVPPTAK
                     SAAADYVYVMDFDGDKIGHMTKIWNDGYTLRQLGWS"
     gene            complement(2954..5821)
                     /gene="leuS"
                     /locus_tag="VGP27_18160"
     CDS             complement(2954..5821)
                     /gene="leuS"
                     /locus_tag="VGP27_18160"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV7853255.1"
                     /translation="MSNESVTDASDAGADTPQFRYTAKLAGEIERAWQQTWSQQGTFH
                     VPNPVGSLAPTDGTPVPADKMFVLDMFPYPSGEGLHVGHPLGYIATDVYARYFRMTGR
                     NVLHALGFDAFGLPAEQYAAQTGTHPRTRTEANIVNFRRQLGRLGLGHDARRSFSTTD
                     VDFYKWTQWIFLQIYNAWFDVDLNRARRIEELVGEFESGRRGLGDGRQWSALSSDERA
                     DVIDAHRLVYRADSMVNWCPGLGTVLANEEVTSDGRSDRGNFPVFRKRLRQWMMRITA
                     YSDRLLEDLDVLEWPEKVKSMQRNWIGRSTGASVSFATDAGDIEVFTTRQDTLFGATY
                     MVLAPEHPLVEELTAADWPDAVNPRWTFGAATPAEAVAAYRRSIAAKSDLERQENKSK
                     TGVFLGSYATNPVNGSQIPVFIADYVLVGYGTGAIMAVPGGDQRDWDFAREFDLPIIE
                     VVAGGDISQAAYNGDGEMVNSGFLNGLSVESAKEAITKRLEADGRGRARVEYKLRDWL
                     FARQRYWGEPFPIVYDENGRAHPLPESELPVELPDIEDYSPVLFEPDDADSVPSPPLN
                     KATDWVHVELDLGDGPKTYTRDTNVMPQWAGSSWYQLRYTDPHNSEAMCDKENEAYWM
                     GPRPSEHGPDDPGGVDLYVGGVEHAVLHLLYARFWHKVLYDLGHVSSREPYRRLVNQG
                     YIQAHAYTDARGTYVPAAEVVEREGRFYWEGPDGEIEVNQEFGKIGKSLKNSVSPDEI
                     GDNYGADTLRMYEMAMGPLEASRPWATKDVVGAYRFLQRVWRLVVDEQTGDLRVTDEE
                     PDDETLRALHRTIAGVTDDYASLRNNTAGAKLIEYTNHLTKEHSTAVPRAAVEPLVLM
                     VAPLAPHLAEELWQRLGHPKSLAHGPFPVADEAYLTVHTVEFPVQVNGKVRGHITVPA
                     DADADAIETAALADEKVQAFLDGAAPKKVIVVAGRLVNLVV"
     gene            6016..6894
                     /locus_tag="VGP27_18165"
     CDS             6016..6894
                     /locus_tag="VGP27_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEV7853256.1"
                     /translation="MKDNARRWRILAGGVAAAMAGVLGLAGNTAAADPLYPYLPQPAP
                     ATVAQTVPVMPGAAAAAQDTGPLGVASSAPAAQSLAGAGVMPAAAAVTPQTLVPAASV
                     PLADFFKEKGVKLEPQVAHDFKALNIVLPVPRGWSQVPDPNVPDAFAVLADRVGGDGI
                     YTSNAALVVYKLVGGSFDPKDAITHGHIDSQQLTAWRTTNASLADFGGMPSAMIDGTY
                     RDNNMTLNTSRRHTIATAGPDKYLVSLSVTTTAAEAVPTANATDAITNGFKISAVGAP
                     ASAAARAPALAQSPPR"
     gene            6929..7294
                     /locus_tag="VGP27_18170"
     CDS             6929..7294
                     /locus_tag="VGP27_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853257.1"
                     /translation="MLFAAVGCLSAAAATAALGAWTLSRPRSADMIRQVLRAVAPTQL
                     AAAAMLTVAGVVALSSQPDTGAVVVAVCVIGAVATVAAGCWQSAKFVAASAIATPACA
                     GVSAGCGTGACATCTLSRN"
     gene            complement(7301..7906)
                     /locus_tag="VGP27_18175"
     CDS             complement(7301..7906)
                     /locus_tag="VGP27_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7853258.1"
                     /translation="MAQSEDPEDFIAPTANRVRAGTLLLANTDLLEPTFRRSVIYIVE
                     HNDGGTLGVVLNRASETAVYNVLPQWAKLATKPKTMFIGGPVKRDAALCLATLRVGTD
                     PSGVPGIRHVQGRMVMVDLDAEPEAIAPLVEGVRIFAGYSGWTIGQLEGEIERDDWIV
                     LSALPSDVLVQARVDLWGRVLRRQPLPLSLLATHPIDISRN"
     gene            7985..>9237
                     /locus_tag="VGP27_18180"
     CDS             7985..>9237
                     /locus_tag="VGP27_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853259.1"
                     /translation="MVDAHAPMALWRSVRSLPEFWRLLELRTASQFGDGLFQAGLAGG
                     LLFNPDRPAGPWEVAGAFAVMYLPYSVLGPFAGALLDRWDRRQVLIGANCARVVLVLA
                     IAALLLAGASDLPILFGALVVNGFTRFVSSGLSAALPDVVPRDQVVTMNSVATATGGA
                     AAFLGAAFMLAPRGLIGADDTGAAAIIFIVVIPVSLALLLSVRFPAHVLGPHRTVRTI
                     HGSVAYAVATGWLHGARTALSVPTVAGTLAGLATHRMVLGINTMLVLVMVRHGDTQSV
                     AGIGTAALFLGAGGVGLFLATLITPGAVGRWGRYRTANGALLLAAAIQLGGVGLQLPV
                     MVLCGFLLGAAGQVVKLCADTAMQIDVDDALRGHMFAVQDAVFWISFIAALTAAAAVI
                     PADGRSPGLVVAGSALYLIGLAVHAG"
BASE COUNT         1409 a         3223 c         3086 g         1519 t
ORIGIN      
        1 ggtcgacgag ctggcagccg agctgcagcg cgtcctgtcc aagcttttct ccgtcttacg
       61 gcgcggcgac gtcaagggca gcaccgcagg aggcgatctg acgctcgcgc agctctctat
      121 cctgttgacc ctgctggaaa agggtcccat ccggatgacc gaccttgcgg cccacgaacg
      181 cgtgcgcact ccgaccacca cggtggcgat ccgccggctg gagaagctgg gcctggtgaa
      241 acggtcgcgc gacgcatcgg atctgcgcgc ggttcttgtc gaggtcacgc cgcgtgggct
      301 ggtccaacac cgggaggccc tcgccgcgcg gcgggatgcg ctggcggcga tgctcggcaa
      361 gctcaccaac gacgacgtcg aaaccctgac caaggcgttg ggtccgatgt cgcggctggc
      421 cggccaggaa agcggccacg aagaaagcta gcccagccag tcgagcaccg cggcggcggt
      481 ccatgactgc tgcatgctgc ccagcggctc cccggtgaac ggttcgtagt actcggcgaa
      541 ggtgccgtcg ctggcctgcc gcaggccttc tcgccgcagg atcagcgacc gctcggccca
      601 gccccggcgg gcgaaggccc aggagaacaa ccaggtcatc accggccaga ccggcccacg
      661 ccagtactcc cgagagcgga agtcgcgaga caccggcgac gtcgacggga tgagggcgta
      721 cctcaggtcc gggtgcccgc agaaccgcgg gccctccagc agccgcagca gcctccgctc
      781 cttgtcgtgc ggcagaccgc cgcacaagag cggggcgaac tgcgcgaccg tctcggtggc
      841 gatccacctg cccgcccgga cgtcgcaatc tcttgccgcg ccggttcttt catcggttgt
      901 ctcgatgacg cccgcccgga atcgctccgc ccacccgtac aggtccctga cgtcggcgtg
      961 cgggcgcttg tagtcctcgc cgatctcggc gaggaccgtg caggcgaccg acagcacagc
     1021 ggagacgaac acgtcctcga cggcgaagct catcaccttg ggcagcaggt cgtcgtcgta
     1081 gtgcgccgac ttcatctctt cgaccagcca caggtagcgg tcgtactcca ggtcggacgg
     1141 acgctggctg gcgtcggtga cgatggtgtt gtcctcgcgc tggtattccg gcacattgcc
     1201 cggaatcaca ttggcgtagg cggcatccca acgcggggaa ttgtccatcc ccgactccca
     1261 cccgtggtac agcgtcaccc tgccgtgcga gttctgatcg cgtgcttcgg ccagccagcg
     1321 gtgccagcgc accagaccgt cccaccggcg gtcgaggaac gcctcggcca ccgcgcgggt
     1381 ggagcgaccg cgcgagcggg catggtcaag gatgcgctgc accgcgatcg cgtgcaccgg
     1441 cggctgcgtg attcccgacg tgtgccgcgt gcgcggcgcg ttgacggcca gcgcagacgt
     1501 cgcccatcgc gcgggccccg ggaagtagcc gtcgaccccg ttggcgaaca cgatgtgcgg
     1561 gatcatcccg ttgctccact gcgccgacag cagggtgtcg agctcgacga cggcccgctc
     1621 gacgctcagc ggggccagcc cgatggacac gaaggcggcg tcccagctcc acatgtgcgg
     1681 gtagagcagc ggcgccgcgg tcgtcatcac acccaggtcg ttaccgcgca gcagatatgc
     1741 cgcgcgcgcc gcgagctgcg tgggagcgaa gctgggatcc ggagccatct cctcaatact
     1801 cgcagtaggt agggtgaccg gcatgccgac cgccttcatc accggaccgg cgggcgggct
     1861 cgggacggcc atcgccgagg ccctcgcacc cagccacacg cttttgctcg gcggccggcc
     1921 gtcggaccgg ctcgacgcgc tcgccgagcg gctcggcgcc acgacctggc cgctggatct
     1981 cgaggatacc gacgccattc ccgccgtcgt ggagcccatc gacgagctcg atgtgctggt
     2041 ccacaacgcg ggcgccgcct tccccggcag ggtcgcggag tcgacggtcg acgagtggcg
     2101 cgtcaccatg gaagtcaatg tcgttggcgc ggtggccctt acgctggccc tattgcctgc
     2161 cttacgcgcc gcccgcggtc aggtgctgtt catcaactcc ggcgcaggga tcaaagcgtc
     2221 gcccggactg gcgtcctata gcgccagcaa gttcgcactg cgtggcttcg cggactcgct
     2281 gcgcaacgac gagccgcagc ttcgggtgac gtccatccat ccggggcgga tcgcgacggt
     2341 gatgcaggag gggctcgtcg cctacgaggg cggcgagtac aagtcggagc gatttctccg
     2401 cccgcagacc gtcgcgaata tcgtggccgc caccgtcaac gccccgcccg aggccgacat
     2461 ccaagaggtc atcgtccggc cccggtgatt tcggtgtgat ttccggcgct cagcgtcggt
     2521 caacgcgtca agatcgctgt atgagcaaaa tgcgagagac cgccgaacaa ttcttcgacg
     2581 cgtgtgagac cggcaagggg tgggacggtt gtgcgccgta ctgccatccc ggcgcgacat
     2641 tcgcaggcca gaccgatgcg ctggccggca tcgacagcct cgaggcgtac accgagtgga
     2701 tgaagaacct gctgacaccg ctgcccgacg gcacggcaga ggtgcgctcg ttcgccgtcg
     2761 atgaagaccg caacaacgta gcggcttacg gaatcttccg cgggacgcac accggcgagg
     2821 gcggcccggt gccgccgacc gccaagagcg cagccgccga ctacgtgtac gtcatggatt
     2881 tcgacggcga caagatcggc cacatgacga agatctggaa cgacggctac accctgcgac
     2941 agctcggctg gagctagacg accaggttga ccaggcggcc ggcgaccacg atcaccttct
     3001 tcggtgccgc cccgtcgagg aacgcctgca ccttctcgtc ggccagcgcg gccgtctcga
     3061 tcgcatcggc gtcggcgtcg gcgggcaccg tgatgtggcc gcgcaccttc ccattgacct
     3121 gcaccgggaa ttcgacggtg tgcacggtca ggtacgcctc gtcggcgacc gggaacggac
     3181 cgtgcgccag cgacttcggg tggccgagcc gctgccacag ttcctcggcg aggtgcgggg
     3241 ccagcggcgc caccatcagc acgagcggtt cgaccgcggc gcgcggcacc gctgtgctgt
     3301 gctccttggt caggtggttg gtgtactcga tcagcttcgc gcccgcggtg ttgttgcgca
     3361 gcgacgcata gtcgtcggtc acccctgcga tcgtgcggtg caacgcccgc agagtctcgt
     3421 cgtccggttc ctcgtcggtg acgcggagat cgccggtctg ttcgtcgacg acaagtcgcc
     3481 acacccgctg cagaaaccgg tatgcgccga cgacgtcctt cgtcgcccac ggccgcgacg
     3541 cctctagcgg gcccatcgcc atctcgtaca tccgcaacgt gtccgcgccg tagttgtcgc
     3601 cgatctcgtc gggcgacacc gagttcttca ggctcttacc gatcttgccg aactcctggt
     3661 tgacctcgat ctcgccgtcg ggcccctccc agtagaacct gccctcccgc tcgacgactt
     3721 cggcggcggg gacgtacgtg ccgcgcgcgt cggtgtacgc gtgcgcctgg atgtaaccct
     3781 ggttgaccag ccggcggtac ggctcccgcg acgacacgtg cccgaggtcg tagagcacct
     3841 tgtgccagaa ccgcgcatag agcagatgca gcaccgcgtg ctcgacgccg ccgacgtaga
     3901 ggtcgacacc gccgggatcg tcaggaccgt gctcggaggg ccgcggcccc atccagtacg
     3961 cctcgttctc cttgtcgcac atggcttcgg agttgtgcgg atcggtgtag cgcagctgat
     4021 accacgagct gccagcccac tgcggcatga cattggtgtc gcgggtgtag gtcttggggc
     4081 cgtcgccgag gtccagctcg acgtgcaccc aatcggtcgc cttgttcagc ggcggcgacg
     4141 ggacgctgtc ggcgtcgtcg ggctcgaaca agaccggcga atagtcttcg atgtcgggca
     4201 gttcgacggg caactcggat tcgggaaggg ggtgcgcgcg cccgttctcg tcgtagacga
     4261 tcgggaacgg ctcaccccag taacgctgcc gggcgaacaa ccagtctcgc agcttgtact
     4321 cgacgcgggc ccggccccgc ccgtcggcct ccaaccgctt ggtgatcgcc tccttggccg
     4381 actcgacgct gaggccgttc aagaagcctg agttcaccat ctcgccgtcg ccgttatagg
     4441 cggcctgcga aatgtcgccg cccgcaacga cttcgatgat cgggaggtcg aattccctgg
     4501 cgaagtccca gtctcgttgg tcgccgccgg gaaccgccat gatggcgccc gtgccgtagc
     4561 cgaccaggac gtagtcggcg atgaagaccg ggatctgcga gccgttgacg gggttggtcg
     4621 cgtaactgcc caggaacacg cccgtcttcg acttgttctc ctgacgctcc agatcggact
     4681 tcgccgcgat cgagcgccgg taggcggcga ccgcctccgc gggggtggcc gccccgaagg
     4741 tccaccgcgg gttcaccgcg tcgggccaat cggccgcggt cagttcttcg acaagcggat
     4801 gctcgggagc cagcaccatg taagtggcgc cgaacagcgt gtcctgccgg gtggtgaaca
     4861 cctcgatgtc accggcgtcg gtcgcgaaag acaccgacgc gccggtggac cggccgatcc
     4921 agttgcgctg catgctcttg accttctccg gccattccag cacgtcgaga tcctcgagca
     4981 gcctgtctga gtaggcggtg atgcgcatca tccactgccg caaccgcttc cgaaacaccg
     5041 ggaagttgcc gcggtcgctg cgcccgtcag aggtgacctc ctcgttcgcc agcacggtgc
     5101 cgaggcccgg gcaccagttg accatcgagt cggcccgata caccaaccgg tgggcgtcga
     5161 tgacgtcggc gcgttcatcc gatgacaacg ccgaccactg cctgccgtcg ccgaggccgc
     5221 ggcgcccgga ttcgaactcg ccgaccagtt cctcgatgcg gcgggccctg ttgaggtcga
     5281 catcgaacca cgcgttgtag atctgcagga agatccactg cgtccacttg tagaagtcga
     5341 cgtcggtggt ggagaagctg cggcgggcat cgtggccgag tccgagcctg cccagctgac
     5401 gccggaaatt gacgatgttc gcctcggtcc tggtgcgcgg atgggtgccg gtctgcgccg
     5461 cgtactgctc tgcgggcagg ccgaacgcgt cgaaacccaa cgcgtgcagc acgttgcggc
     5521 cagtcatccg gaaatagcgg gcgtagacgt cggtcgcgat gtagccgagc gggtgcccga
     5581 cgtgcaatcc ctcacccgac gggtagggga acatgtccag gacgaacatc ttgtcggcgg
     5641 gaaccggcgt tccgtccgtc ggcgccagcg agcccaccgg gttgggcacg tgaaatgtgc
     5701 cctgctgcga ccacgtctgc tgccaggcac gctcgatctc cccggccagc ttcgcggtgt
     5761 agcggaactg cggggtgtcg gctccagcat cggaagcgtc ggtgaccgac tcgttactca
     5821 cgtgaacagg gtaaagggcc gccggggacc gcccgtcggc cacgggcagg tatcggttgc
     5881 attgcggagc ggtcagaggt tcattccgac tcggtcccag gcctggtggc cgactccgcc
     5941 ccggggttac ggtcgccccc tggccagggg catgtctcgc cccgcagccg aaaggactcg
     6001 ggaggggatc gggcgatgaa agacaatgcc cgccgctggc ggatcttggc aggcggtgtc
     6061 gccgcggcta tggccggcgt gctcggcctg gccggcaaca ccgccgcagc cgacccgctc
     6121 tatccctatc ttccgcagcc cgcgcccgcg accgtcgccc agacggtgcc cgtcatgccc
     6181 ggtgccgccg cggccgccca ggacaccggc ccactcggcg tcgcatcctc cgcacccgcc
     6241 gcccagtcgc tggccggtgc cggcgtgatg cccgccgcgg ccgcggtgac gccccaaacg
     6301 ctggtgcccg ccgcctcggt gccattggca gatttcttca aggagaaggg cgtcaagctc
     6361 gaaccccagg tggcccacga cttcaaggcg ctcaacatcg tgctgcccgt cccgcgcggc
     6421 tggtcgcagg tgccggaccc gaacgtgccc gacgcgttcg cggtgctcgc cgaccgggtc
     6481 ggcggcgacg gcatctacac ctcgaacgcg gcactggtcg tctacaaact cgtcggcggc
     6541 agcttcgatc ccaaggacgc catcacccac ggccacatcg attcccaaca gctgaccgca
     6601 tggcgcacca ccaacgcgtc gctggccgac ttcggtggca tgccgtcggc gatgatcgac
     6661 ggcacctacc gggacaacaa catgacgctc aacacgtcgc gcaggcacac cattgccacc
     6721 gccggtcccg acaagtacct cgtgtcgctc tcggtcacca ctaccgccgc tgaagccgtc
     6781 cccaccgcga acgccaccga cgcaatcacc aacggattca agatcagtgc cgtcggcgca
     6841 cccgcttcag cggcggcacg ggcgccggcg ctcgcgcagt caccgccccg gtagcgcgac
     6901 atgggcggtg ccccctaagg taggccgcat gctcttcgcg gcggtgggtt gtttgtctgc
     6961 cgccgccgcg accgccgccc tcggcgcgtg gacgttgtcg cgtccccgct ccgccgacat
     7021 gatccggcag gtcctgcgcg cagtcgcacc gacacagctc gccgccgcgg cgatgctgac
     7081 cgtcgcggga gtggtggcgc tgtcctcgca gccggacacc ggcgcggttg tcgtcgcggt
     7141 ctgcgtgatc ggcgcggtgg ccacggtagc cgccggttgc tggcagagcg cgaagttcgt
     7201 cgcggcgagt gcgatagcga cacccgcttg tgcgggagtg tcggcgggat gcggaaccgg
     7261 cgcctgcgcg acctgcaccc tgtcgcgcaa ctaggcgaaa ctagttgcgg ctgatatcga
     7321 ttgggtgtgt cgccagcaac gacaacggca gcggctggcg ccgcaacacg cgtccccaca
     7381 gatccaccct cgcctggacc agaacgtcgg atggcagcgc cgacaatacg atccagtcgt
     7441 cgcgctcgat ctcgccctcg agttggccga tcgtccagcc cgagtacccg gcgaagatgc
     7501 gcacgccctc gaccagcggc gcgatcgcct cgggttcggc gtccaggtcg accatcacca
     7561 tccggccctg cacatgccgg atgcccggca ctccgctcgg atccgtgccg acgcgcaatg
     7621 tcgcaagaca cagcgcggcg tcgcgcttca ccggaccgcc gatgaacatc gtcttcggct
     7681 tggtggcgag cttggcccac tgcggcagca cgttgtacac ggcggtttcg ctggcccggt
     7741 tcagaaccac cccgagtgtg ccgccgtcgt tgtgctcgac gatatagatg acgctgcggc
     7801 ggaaggtcgg ttcgagcaga tcggtgttgg ccagcagcag cgttccggcg cgcacccggt
     7861 tcgccgtagg agcgatgaag tcctccggat cttctgactg cgccacctga ccatcatgtc
     7921 accagcgaac catcaaggtg gcgaacaagc gcgggccgcc gggatatttg tactgtgaat
     7981 ccctgtggtt gacgctcacg caccgatggc cctgtggcgg tctgtgcgca gccttcccga
     8041 gttctggcga ctgctcgaac tgaggacggc gagccagttc ggcgatggcc tgtttcaggc
     8101 cggcctcgcg ggcggattgc tgttcaatcc cgaccgcccg gcaggcccat gggaagtcgc
     8161 cggcgcgttc gccgtcatgt atctgccgta ctcggtgctg ggtccgttcg caggcgcgct
     8221 gctggaccgg tgggacaggc gacaggtgct gatcggcgcg aactgcgcgc gggtcgtcct
     8281 cgtgctcgcg atcgccgccc tactgttggc cggcgccagc gatctaccca tcctgttcgg
     8341 cgcgctggtc gtcaacggct tcacccgctt cgtgtcctcg ggcctgtcgg ccgcactgcc
     8401 cgacgtggtg ccccgcgacc aggtggtcac gatgaactcc gtcgcgacgg ccaccggcgg
     8461 tgctgcggcg ttcctcggcg ccgccttcat gttggcgccc cgcggactga tcggggccga
     8521 cgacacgggc gccgcagcaa tcatcttcat cgtcgtgatt ccggtctcgc tggcgttgct
     8581 gttgtcggtg cgcttccccg ctcatgtact cgggccgcac cgaactgtgc gcacgattca
     8641 cgggtcggtg gcctacgccg ttgcgaccgg ctggctgcac ggcgcgcgca cggcgctgtc
     8701 ggtgccgact gtcgcgggca cgctcgccgg gctggccact caccggatgg tgctcgggat
     8761 caacaccatg ctggtgcttg tcatggtgcg acacggcgac acccagagcg tcgcgggcat
     8821 cggcaccgcc gcgctgttcc tcggcgcagg cggcgtcggg ttgtttctgg cgacgctgat
     8881 cacgccgggc gccgtgggcc gatggggccg ctaccggacc gccaacggag cgctgttgct
     8941 ggcggcggcc atccagctcg gcggcgtcgg tctgcagctg cccgtaatgg tgctgtgcgg
     9001 gttcctgctc ggcgcggccg gtcaggtcgt caagctgtgc gccgacaccg cgatgcagat
     9061 cgatgtcgac gacgcgctgc gcggccatat gttcgccgtc caggatgcgg tgttctggat
     9121 ctcgttcatc gccgcgctca cggcggcggc ggcggtcatc cccgccgacg gccggtcccc
     9181 cgggctcgtc gtcgccggtt cggcgctcta cctcatcggc ctcgcggtgc atgccgg
//
LOCUS       DASYLX010000777         4710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1798538,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000777 DASYLX010000000
VERSION     DASYLX010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798538"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGP27_18185"
     CDS             complement(<1..722)
                     /locus_tag="VGP27_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7853260.1"
                     /translation="MADPDVLLEVTDLVKHFPIRSGVVVDREVARVRAVDGVSFTLRE
                     GETLGLVGESGCGKSTLCRAILQLTTPTSGSVRFAGQELVGRSRRELRPLRRQMQMIF
                     QDPFASLNPRKRVGQIIGDPIELHGLARGAELKRRVQDLLDRVGLNAEHYNRFPHEFS
                     GGQRQRIGIARALALRPKLIVADEPVSALDVSVQAQIINLLEDVQDEFGLSYLFVAHD
                     LGVVRHVSDRIAVMYLGKIVEDS"
     gene            complement(715..1806)
                     /locus_tag="VGP27_18190"
     CDS             complement(715..1806)
                     /locus_tag="VGP27_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853261.1"
                     /translation="MNAILTVDDLRVSFATEDGVLTAVDGVSFELDAGEVLAIVGESG
                     CGKSVTAQTLTGLTQASNARITGSVNYRGREINGLSDNQLRDVRGEQIAMVFQDPMSA
                     LNPVYRVGDQITEMIRAHRDVSKKEARSQAVELLRSVGIPNPERRVRHYPHEFSGGMR
                     QRVMIAMALALEPDVLIADEPTTALDVTIQAQILQLIDRLNRERGLAVIIITHDLGVV
                     AEIADRVLVMYAGQVVEDATLDEIFYEPKHPYTWGLLGSLARLDQPRTTRLAQIPGAP
                     PSLLSPPPGCRFAPRCTHVFDQCAELPPLEPRSGGGHLDRCWLDAEVKAQHGRAREEQ
                     NGWRAREEQNGCRAREEQNGGRAREEQNG"
     gene            complement(1803..2765)
                     /locus_tag="VGP27_18195"
     CDS             complement(1803..2765)
                     /locus_tag="VGP27_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853262.1"
                     /translation="MARFVARRLLGMVAVLFAISVIVFLIFNVIPNSDPAARIAGKNA
                     DPALIARVNADLGLDRPLPIQYLTMMKQIFTGQLTSYAKDRNVVQQIWEGLPATFSLC
                     IGAAVLWMSLAILFGYLSAVHAGRFTDRALTILSLVGISMPVFWLAAMLLYFLSFKVQ
                     AFPTGSYVPFSDDPVDWAYHLILPWFTLAVLFVGIYSRVLRSNMLDAMNEDYVRTARA
                     KGLSERQVRIRHVLRNSMIPVITLFGLDFGMVVGGGAILTETVYNLDGVGLYAGEAIR
                     SLDLPPLMAVVMFGAFFIVLFNTIVDILYAVLDPRIRLGEAAPA"
     gene            complement(2769..3806)
                     /locus_tag="VGP27_18200"
     CDS             complement(2769..3806)
                     /locus_tag="VGP27_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853263.1"
                     /translation="MVSPIDSLEAPGEPPLPTGVPSAQIQGRSPWYLAWLRLRRNKVA
                     LVCAVLFVLIVLFCLAAPLWAEHVAHTGPNENHLTDKVMIDGQETDVVAPDGTPLGPG
                     LHGKFLLGADQNGRDVMVRLMYGGRTSIYVGLAAAAVTTILAVVVGLLAGYYRGWIDS
                     VLSRAMDVIWAFPVLLLGIALGTTLSIGGLKAGPIEIAGDSLWIPILIIGLVYVPYVA
                     RPIRGEILALREKEFIEAAVAQGKGPLRIMFSELLPNIVSTIVVFFTLNIANNMLLES
                     ALSFLGAGVRPPNASWGTMISDGYQVIFTAPHLTIVPGLMIVATVLSLNIFGDGLRDA
                     MDPRAKIRLEH"
     gene            complement(3813..>4710)
                     /locus_tag="VGP27_18205"
     CDS             complement(3813..>4710)
                     /locus_tag="VGP27_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7853264.1"
                     /translation="DKIVVNENKNNSAQVTDVEQNKVDFMLDPPVADRLGEVKAKFSD
                     RFRMEESINTYYFFMNTQRAPFNDIKVRQAINYAVDPEALNRIFGGRMHPTQQILPPG
                     MPGYQEYKLYPGPDMNKAKQLIAEANPADRDITVWTDDEPDRKRIGEYYHDLLTQLGF
                     NATLKVIAGDVYWTTIGNQSTPDVDTGFADWFQDFPHPDDFFRPLLNGASILPTNGNN
                     YSRVNIPANDAKMDELLTKLIGDGGVEDQYAALDKAYMEQAVWAPYGNEQFTTFLSER
                     MDFDKSYHHLLFNQDFTSLAVK"
BASE COUNT          851 a         1498 c         1536 g          825 t
ORIGIN      
        1 gagtcctcga cgatcttgcc cagatacatg accgcgatcc ggtcggatac gtgacggacg
       61 acgccgaggt cgtgggcgac gaaaagatag gacagcccga actcgtcctg cacatcctcg
      121 agcaggttga tgatctgtgc ctgcacggag acatcgagtg cggagacggg ttcgtcggcg
      181 acgatcagct tgggccgcaa cgcaagtgcg cgggcaattc cgatgcgttg acgctgaccg
      241 ccggagaact cgtgcgggaa gcggttgtag tgctcggcgt tcaggccgac gcggtcgagc
      301 aggtcctgga cgcgccgttt gagctccgcg ccccgtgcca gcccgtgcag ttcgatcggg
      361 tcgccgatga tctggcccac ccgcttgcgc gggttgaggg acgcgaacgg gtcctggaag
      421 atcatctgca tctgtcgccg caacgggcgc agctcgcggc gcgagcggcc gaccagttcc
      481 tggcccgcga accgtaccga cccggaggtc ggagtcgtca gttgcaggat cgcccggcac
      541 agcgtcgact tgccgcagcc ggattcgcct accaggccca gtgtctcgcc ttcccgcaga
      601 gtgaagctga cgccgtcgac tgctcgcacc ctggcgacct cgcggtccac gacgaccccc
      661 gacctgatcg ggaagtgttt gacgagatcg gtaacctcca gcagcacatc cgggtcagcc
      721 attttgctcc tcgcgtgcgc ggccgccgtt ttgctcctcg cgtgcgcggc acccattttg
      781 ctcctcgcgt gcgcgccacc cattttgctc ctcgcgtgcg cggccatgct gcgccttaac
      841 ctccgcgtcg agccagcacc ggtcgaggtg cccgcccccg ctgcgtggtt cgaggggcgg
      901 aagctcggcg cactggtcga agacgtgggt gcagcgggga gcgaagcgac aacccggcgg
      961 cggggacagc agcgacggcg gcgcccccgg aatctgcgcc agccgcgtgg tgcgcggctg
     1021 atcgagccgg gcgagtgagc cgagcagacc ccaggtgtag ggatgcttcg gctcgtagaa
     1081 gatttcgtcg agcgtcgcgt cctcgacgac ctgacccgca tacatcacca acacccggtc
     1141 ggcgatctcg gcgacgacgc ccaagtcgtg ggtgatgatg atgacggcca ggccgcgctc
     1201 gcgattgagc cggtcgatca gctgaaggat ctgcgcctgg atcgtcacgt ccagcgcggt
     1261 cgtcggctcg tcggcgatca gcacgtcggg ttcgagcgcg agcgccatgg cgatcatgac
     1321 gcgctggcgc atgcctccgg agaactcgtg gggatagtgc cgtacccgcc gctccggatt
     1381 cggaataccc accgaccgca gcagttcgac cgcctgcgag cgggcctcct tcttcgagac
     1441 gtcgcggtgc gccctgatca tctcggtgat ctggtcgccc acgcggtaaa cggggttcag
     1501 ggcgctcatc ggatcctgga agaccatggc gatctgctcg ccgcgcacgt cgcgcagttg
     1561 gttgtcactg agcccgttga tctctcgacc gcgatagttg accgagccgg tgatcctggc
     1621 gttcgacgcc tgggtcaaac ctgtcaatgt ctgcgcggta acgcttttgc cgcatccgga
     1681 ttcgccgacg atcgccagca cctcgcccgc gtccaactcg aacgagacgc cgtcgaccgc
     1741 cgtcaacacg ccgtcctcgg tggcgaagct gacgcgaagg tcgtcgaccg tcaggatcgc
     1801 gttcatgcag gcgccgcctc tccgagccga atgcgcggat cgagcaccgc gtacaggatg
     1861 tcgacgatcg tgttgaacag cactatgaag aaggccccga acatcaccac ggccatcagg
     1921 ggcggcagat ccagcgaccg gatcgcctct cccgcataca gaccgacgcc gtcgaggttg
     1981 tagacggtct cggtgaggat ggcaccgccg ccgaccacca ttccgaagtc cagcccgaac
     2041 agcgtgatga cggggatcat cgagttgcgc agcacgtgcc ggatccgtac ctgacgttcc
     2101 gacagaccct tggccctggc ggtacgtacg tagtcctcgt tcatcgcgtc gagcatgttg
     2161 gaccgcagca cgcggctgta gatgccgacg aacagcacgg ccagcgtgaa ccacggaagt
     2221 atcaggtgat acgcccagtc caccggatcg tcggagaacg gcacgtaact gccggtcgga
     2281 aacgcctgca ccttgaagct gaggaagtag agcagcatcg ccgccagcca gaacaccggc
     2341 atcgaaatgc ccaccagcga aaggattgtc agggcgcggt cggtgaacct gcctgcatgg
     2401 acagcgctca gatagccgaa caggatggcc agcgacatcc acagcaccgc cgcgccgatg
     2461 cacagcgaga acgtggcagg caggccctcc cagatctgtt gaacgacgtt gcggtccttg
     2521 gcatacgacg tcagttggcc ggtaaagatc tgcttcatca tcgtcaggta ctggatcggc
     2581 agcggcctgt cgaggccgag atcggcgttg acccgggcga tcagcgccgg gtcggcgttc
     2641 ttgcccgcga tccgggccgc cggatccgag ttcgggatga cgttgaagat caggaacacg
     2701 atcaccgaga tcgcgaacag cacggcgacc attcccagta gccgcctggc gacgaagcgg
     2761 gccatcggtc agtgctccag ccggatcttc gcacgcggat ccatcgcgtc acgcaggccg
     2821 tcaccgaata tgttgagcga cagcacggtt gcgacgatca tcaatccggg cacgatggtg
     2881 aggtggggcg cggtgaagat cacctgatac ccgtcggaga tcatggtccc ccacgaggcg
     2941 ttgggcggtc gcaccccggc gcccaggaac gacagcgcgg attcgagcag catattgttg
     3001 gcgatgttca gcgtgaagaa gaccacgatg gtggacacga tgttgggcag caactcggag
     3061 aacatgatcc gcagcggacc cttaccctgg gcgacagccg cctcgatgaa ctccttctcc
     3121 cgcagcgcaa ggatctcgcc gcgaatgggc cgggccacgt agggcacata caccagcccg
     3181 atgatcagga tcggtatcca cagggagtcg cccgcaatct cgatcggacc ggccttcagg
     3241 ccgccgatgg acaacgtggt gcccagggcg ataccgagca gcagtacggg aaatgcccag
     3301 atgacgtcca tcgcccgcga cagcacggag tcgatccagc cgcggtagta gccagccagc
     3361 aggccgacga cgacggccag gatcgtcgtc accgcggccg ccgccagtcc gacgtagatc
     3421 gatgtcctgc cgccgtacat caaccgcacc atgacgtctc ggccgttctg atcggcgccg
     3481 agcaggaact tgccgtgcag gcccggcccc agcggcgtgc cgtcaggggc gacgacgtcg
     3541 gtctcctgcc cgtcgatcat taccttgtcg gtcagatggt tctcgttcgg gccggtgtgc
     3601 gcgacgtgct cggcccacag cggcgccgcg aggcagaaca gcacgatcag cacgaacagg
     3661 acggcgcata ccaatgcaac cttgttgcgc cgcaaccgga gccacgccag ataccaggga
     3721 ctgcggccct gaatctgagc cgacggcaca ccggtcggga gcggcggttc accgggcgcc
     3781 tccagcgaat cgatcggcga gaccatgtcc gtctacttga cggcgaggga ggtgaagtcc
     3841 tggttgaaga gcagatggtg ataggacttg tcgaagtcca tgcgttccga gaggaacgtg
     3901 gtgaactgtt cgtttccgta cggcgcccag accgcctgct ccatgtaggc cttgtcgagc
     3961 gcggcgtact ggtcctccac gccgccgtca ccgatcagct tggtcagcag ctcgtccatc
     4021 ttggcgtcgt tggccgggat gttgacccgg gaataattgt tgccattggt gggcaggatg
     4081 cttgcaccgt tgagtagcgg acggaagaag tcgtccggat gcggaaagtc ctggaaccag
     4141 tccgcaaacc cggtgtccac atcgggggtc gactggttgc cgatcgtcgt ccagtacacg
     4201 tcgcccgcaa tcactttcag cgtggcattg aatccaagct gggtgagcag gtcgtggtag
     4261 tactcgccga tgcgcttgcg gtcgggctcg tcgtcggtcc agaccgtgat gtcgcggtcg
     4321 gccgggttcg cctcggcgat cagctgtttc gccttgttca tgtccgggcc gggatacagc
     4381 ttgtactcct ggtatccggg catgcccggg ggcaggatct gctgtgtcgg gtgcatgcgg
     4441 ccaccgaaga tccggttgag agcttcggga tcgaccgcat agttgatggc ctggcggacc
     4501 ttgatgtcgt tgaacggggc ccgctgggtg ttcatgaaga agtagtaggt gttgatcgat
     4561 tcctccatcc ggaaccggtc gctgaacttc gccttcacct ccccgagccg gtccgcgacg
     4621 ggcggatcga gcatgaagtc gaccttgttc tgctcgacgt cggtcacctg ggcgctgttg
     4681 ttcttgttct cgttgacgac gatcttgtcg
//
LOCUS       DASYLX010000778         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1029860,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000778 DASYLX010000000
VERSION     DASYLX010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029860"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VGP27_18210"
     CDS             complement(<1..172)
                     /locus_tag="VGP27_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853265.1"
                     /translation="MRLGFLLNEVLTGLRRNVTMTIAMILTTAISIGLFGGGLLVVRL
                     ADNSRAIYLDRVE"
     gene            complement(172..861)
                     /gene="ftsE"
                     /locus_tag="VGP27_18215"
     CDS             complement(172..861)
                     /gene="ftsE"
                     /locus_tag="VGP27_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV7853266.1"
                     /translation="MITLDHVSKHYKSSARPALDNVSLRIDKGEFVFLIGPSGSGKST
                     FMRLLLAEESPTNGDVRVSKFHVNKLPGRHIPSLRQVIGCVFQDFRLLQQKSVFENVA
                     FALEVIGKRPDVINRVVPDVLEMVGLSGKANRLPGELSGGEQQRVAIARAFVNRPLLL
                     LADEPTGNLDPETSKDIMDLLERINRTGTTVLMATHDHHIVDSMRQRVVELSLGRLVR
                     DEQRGIYGMDR"
     gene            complement(907..1362)
                     /locus_tag="VGP27_18220"
     CDS             complement(907..1362)
                     /locus_tag="VGP27_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853267.1"
                     /translation="MTSWFKAKLGGRFRVSTVVLLIAFIALFWVQQTFQPEPAPEAPA
                     SSVVPPGFVPDPNYTWVPRTQVRRPKEPNYTTTTTPTTTTTETTTPSPDTTTSSTETS
                     PTPTTTVVDPDGPFGPLGPQTLTQTPTVTAPTTAPPVPGTVPATPEAPR"
     gene            complement(1359..>1776)
                     /locus_tag="VGP27_18225"
     CDS             complement(1359..>1776)
                     /locus_tag="VGP27_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV7853268.1"
                     /translation="NGQIVKTLNLSKDWARAVVDIPVPTSADLNVVNDLLHDVAEKAM
                     EDPALKDLLLDAPQLMGVESIELDTVNLRMVARTLPGKQYEVGRRIRVLVVRALRRAG
                     IASTDGTSPMVAAIPHPAEAPAAEEAETQQAPEQRK"
BASE COUNT          292 a          571 c          598 g          315 t
ORIGIN      
        1 tctcgacccg atccaggtag atggcgcgcg agttgtcggc gaggcgcacc accagcagac
       61 cgccgccgaa caagccgatc gagatcgcgg tggtcaggat catcgcaatc gtcatggtga
      121 cgttgcgacg aaggccggtc aggacctcgt tgagcaggaa gcctaagcgc actagcggtc
      181 cattccgtag atgccgcgct gctcgtcacg aacgagacgg ccgaggctga gctcgacgac
      241 gcgctgccgc atcgagtcga cgatgtgatg gtcgtgagtc gccatcaaca cggtggtccc
      301 cgtgcggttg atgcgctcga gcagatccat gatgtccttg ctggtctcgg gatccaggtt
      361 tcctgtcggc tcgtcggcca gcagcaggag cgggcggttg acgaaggcgc gggcgatcgc
      421 cacccgctgc tgctcgccgc cggacagctc gccgggcaac cggttcgcct tccccgacag
      481 cccgaccatc tcgaggacgt cgggcaccac ccggttgatc acatcgggcc gcttgccgat
      541 cacctcgagg gcaaaggcca cgttctcgaa gaccgacttc tgctgcagca gccggaagtc
      601 ctgaaagaca cacccgatga cctgccgcag gctggggatg tggcggccgg gcagcttgtt
      661 gacgtggaac ttcgacaccc gcacgtcgcc gttggtcgga gactcctcgg caagcagcag
      721 tcgcatgaag gtcgacttgc ccgaaccgga tggaccgatg agaaaaacga actcaccctt
      781 gtcgatccgg agcgagacat tgtccagcgc agggcgtgcc gaggacttgt aatgcttgct
      841 tacgtggtcg agcgtgatca tcacggcacg ccagtgtagc ggtgagacaa ggcagggtcc
      901 gtcgagctat ctcggcgcct caggcgtggc gggcaccgtt cccggtacgg gcggggccgt
      961 cgtcggcgcc gtcaccgtcg gtgtctgagt cagcgtctgc gggccgagcg ggccgaaggg
     1021 accgtccgga tcgacaaccg tcgtcgtcgg ggtcggcgac gtctcggtcg aactggtcgt
     1081 ggtgtccggc gatggcgtcg tcgtctccgt cgtggtggtc gtcggcgtcg tcgtggtggt
     1141 gtagttcggc tccttgggcc gccgaacctg cgtgcgcggc acccaggtgt agttcgggtc
     1201 gggcacgaag cccggcggaa cgaccgagga agcaggcgct tctggggccg gttccggctg
     1261 gaacgtctgc tgcacccaga acagcgcgat gaatgcgatc aagagcacca cggtcgacac
     1321 ccggaagcgg ccgccgagct tcgccttgaa ccaggaggtc actttctctg ctccggtgct
     1381 tgctgggtct ccgcttcctc cgcggcaggc gcctccgcgg gatgtggaat cgctgcgacc
     1441 atcggtgacg tgccgtcggt tgacgcgatg ccggcccgac gcaacgccct gaccaccagc
     1501 acgcggatcc gccgacccac ctcgtactgc ttgcccggca gcgtgcgcgc caccatgcgc
     1561 aggttcacgg tgtccagttc gatgctttcc acacccatca gctgtggcgc atcgagcagc
     1621 aggtccttga gcgccgggtc ttccatcgcc ttctcggcca catcgtgcag caggtcgttg
     1681 acgacgttca ggtcggccga tgtcggaacc ggaatgtcga cgaccgcgcg cgcccagtcc
     1741 ttggacaggt tcagcgtctt gacgatctgc ccgttg
//
LOCUS       DASYLX010000779         6696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1688850,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000779 DASYLX010000000
VERSION     DASYLX010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688850"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGP27_18230"
     CDS             <1..417
                     /locus_tag="VGP27_18230"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV7853269.1"
                     /translation="AVGVFDPLAGALSYGLAGHPPALLRRARDGAVVRLVDAHGPVLG
                     PIRDATYTEGQVRLSPGDILLMYTDGLVECPGRDIETGIAQVQRLLAGWRSDTALAEG
                     CRQLTETLALPPRQDDVCVIAVRVRVADSTLPAAPS"
     gene            828..980
                     /locus_tag="VGP27_18235"
     CDS             828..980
                     /locus_tag="VGP27_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853270.1"
                     /translation="MATLAYAATQSLDVEQAQTFIDGLVASVPMLAGAVKRRVTPSPG
                     AEQPYL"
     gene            1485..2471
                     /locus_tag="VGP27_18240"
     CDS             1485..2471
                     /locus_tag="VGP27_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853271.1"
                     /translation="MRIITVKEHFQHQEAVARVLELSDPPPAQPDAGFGAFLRDFTPD
                     KDSTERLAGKRLKHMDEGGIDMQVVSHGANGPSTLDHPEAVYLCRKVNESLAEQISQH
                     PTRFRGFATIPLHDPRAAADEMRRCANELGFVGTLVMGTYGGLFLDDAPVLSAAEEVD
                     LPIYVHPGVPPNRVTTAYYAGSWPTAIQFLFSSPAFGWHAEAGIHVLRLVLSGALDRH
                     PGLKLLSGHWGELVAGWLDRLDETIGWAKYLDRPVSDYYRQHVWITPSGMYSQNQLKF
                     FVAEMGADRIIHSEDFPYVVRDNVSEFIEQADLSDDERTAIAHRNAEKVMRI"
     gene            2612..3937
                     /locus_tag="VGP27_18245"
     CDS             2612..3937
                     /locus_tag="VGP27_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853272.1"
                     /translation="MALLVAAAFFMEILDATIIAPAIPRIAESFGVAAVNINVAISAY
                     LITVAVLIPATGWMADRFGVRRVFVTAIAVFTIASVGCAASTSLPMLVGMRVLQGVGG
                     AMMVPVGRLAVLRHSGKSDLVRAIALLTWPALAAPVIAPALGGAIATFVSWRWIFLIN
                     IPIGVIGFLLALRLVHGEPVPSARPLDWRGMLMIGLGIASALIALEQLHVSGTDWALV
                     GVSGAAAVVLLGCAARHLLRSDAPLVQLRVLRERTLRITVTAGSMYRMVITAVPFLLP
                     LQFQFVFGWTPFSAGLMVAALFLGNLTIKPATTPLMRRFGIRNVLLANGMASVGCFGL
                     LALLAPGLPIVAMAAILYVSGALRSIGFTAYASLAYSGIEGDDLTHGNTLNSSVQELA
                     AGLGIAIAALLLSILSSYPYVYLVLGAVLATTLIETLRLPRDAGSHVSG"
     gene            complement(3942..4808)
                     /locus_tag="VGP27_18250"
     CDS             complement(3942..4808)
                     /locus_tag="VGP27_18250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7853273.1"
                     /translation="MRVGVTFPQTELGGDAGAVRAYAEGVEELGYRHILVYDHVVGAD
                     PKVHTGWNGPYDVHTTFHEPFVLYGYLAAVTRSLELVTGVIILPQRQTALVAKQAAEV
                     DLLSGGRFRLGIGVGWNAVEYEALGEDFGSRGKRSAEQIALLRRLWTEQSVTFDGEFD
                     KLTGAGIAPLPVQRPIPVWVGAASPPGYKRAGRLADGWFPMMGPGPALDEARTVVERA
                     AVAAGRDPASIGMEGRVDWRGDVDGVIERLKQWAEAGATHASVNTMGAGLRTVDDHLA
                     ALSRVAESLPAG"
     gene            4889..5650
                     /locus_tag="VGP27_18255"
     CDS             4889..5650
                     /locus_tag="VGP27_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV7853274.1"
                     /translation="MSDDRDYPQMAASRGRIEPAPRRVRGFLGHELVFDTTHARYVWE
                     VPYYPQYYIPLTDVRTEFLRDEDHPQKVQLGSSRLHSLIGAGHTHEKAARVFDDGPVA
                     GLVRFEWDPLSWLEEDEQIYGHPRNPYARVDALRSHRHVQVRLDDVVLADTVAPVLLF
                     ETGLPTRYYIDYSDVAFEHLEPSDTQTLCPYKGTTSGYWSVRTADTLHPDLAWTYHYP
                     LPAVAPIAGLMAFYNEKLDVVVDGVSLARPKTHFS"
     gene            5775..6362
                     /locus_tag="VGP27_18260"
     CDS             5775..6362
                     /locus_tag="VGP27_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7853275.1"
                     /translation="MMARITPADGFAAIPTTDIGQGDRINLGVAGIMSRLPDVAAAVG
                     GVTGALREAGALSPRLMELVRLRIAFHNQCRSCMSIRYQSAIDDGLTEDAVCSLERPA
                     EASDLTDPERSALRFADLFATDHLAIGDSVYDDLRRHFTEDQLVALGLHCALCVGLGR
                     LAATWHVVDDLPDEYRADSGIPLAPWSANSVVASG"
     gene            complement(6400..>6696)
                     /locus_tag="VGP27_18265"
     CDS             complement(6400..>6696)
                     /locus_tag="VGP27_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV7853276.1"
                     /translation="VPNTDDNSVSVVDLNSRAVIQTIAVGQQPSAGTVDSEAHTVLVT
                     NHGAASVSVIDSQKRVVTDSVGVGDGPQTVAADPATHTAWVVDFRANSISVIER"
BASE COUNT         1128 a         2211 c         2108 g         1249 t
ORIGIN      
        1 gccgtgggcg tcttcgatcc gctggccggc gccttgtcct acggcctggc cgggcatcca
       61 ccggcattgc tgcgtcgcgc gcgggacggg gcagtcgtgc gactcgtcga cgcccacggc
      121 ccggtgctcg ggcccatccg tgatgccacc tacaccgagg gccaggtgag gcttagtccc
      181 ggtgacatct tgctcatgta caccgacggc ctggtcgagt gcccaggccg cgacatcgag
      241 acaggcatcg cgcaggtgca gcggctattg gcgggctggc gatcggatac cgcgctggcc
      301 gagggctgcc ggcaactcac cgaaacgctt gccttaccgc ctcgccaaga cgatgtctgt
      361 gtgatcgccg tgcgcgtccg ggtcgccgat tccacgctgc cggcggcgcc gagctgagcg
      421 tcgcgacgtc tacgccggtc gaggatcgga ccgggtgagc gcatgacgtc gactaaaaag
      481 acaagtgttt cccgaatttt ccgccgaggc cgcagagcga gcgtgcctgc aatcacgtcg
      541 cggcgacgcg ccgtcgtgga acaggtggac atcgacgggg gtgtcgtctc agcgattccg
      601 tccgccgccc aggtcaccgt cggcatcatc ggagcggtgc tcgagataga gcaggactat
      661 atagatcagc gatcagcatg ctggacagcg atgttacggc cgtacacgaa aaatcacctg
      721 atcaccggac ggccttggcg gcaagagggc gtcaactatc gcgtcgaatt gttgactctc
      781 gagattcctt gctatgcaag ggtttccaat acgaagaggc gcgcaatgtg gccaccctcg
      841 catatgccgc aacgcaatcg ctcgacgtcg aacaggcgca aaccttcatt gacggcctgg
      901 tcgccagtgt gccgatgctg gcgggcgctg tgaaacgtcg cgtcactcct tctccgggag
      961 ccgaacagcc ttacctctga gtcggagacc cgcagacttc gatgcccggc acggcgcata
     1021 gtccgactgt tccaccgaac cgcacagtcg cctggttgac gtttgggtta aatgttgctc
     1081 tgaggtcgtt gagtcggcgt tcgactacgt agcgttgagg catggagggg gcagccgatc
     1141 tgcgcagcac atttcagaag tcgctgaact gtgcgatcgc gcatggtcag cgcccttcac
     1201 ctcggtgcgg actgggagac aggacgcaaa cagcgatcga agctgtcgcg caggagcatc
     1261 cagatgcgac ggtcgaccac atcgccgatg cctacgatgc gttcgctcgc gaacacgagc
     1321 cgtccagttc cacggcgccg caggcgaatg agctgaagcg attagaggca agtcgcgaca
     1381 ggttcaacca aagctgagac agccgctgtc cagtttccgg cgtacgaatg agggtgaggt
     1441 catcgaacct cgcccgttag acattcacgt cgtgaggaag tccattgcgc atcatcaccg
     1501 tcaaggagca tttccagcac caggaggccg tcgcccgcgt tctggaactg tcggacccgc
     1561 cgcccgctca gcccgacgcc ggcttcggcg cattcctgcg ggacttcacg cccgacaaag
     1621 actcgacgga gcggttggcg ggcaagcggc tgaagcacat ggacgagggc ggcatcgata
     1681 tgcaggtggt gtcgcacggc gcgaacggcc caagcactct ggatcacccc gaggctgttt
     1741 atctgtgccg caaagtcaat gagtcactcg ccgaacagat ttcacaacat cctactcgat
     1801 ttcgcggctt tgcgaccatt ccgctgcacg acccccgcgc ggctgccgac gagatgcggc
     1861 gatgcgccaa cgagctcggg ttcgtgggaa ccctcgtgat gggaacctac ggcgggctgt
     1921 tcctcgacga tgcccccgtc ctgtccgccg ccgaggaagt cgatctcccg atctacgtcc
     1981 accccggtgt gccgccgaac cgggtaacga ccgcttacta cgcgggaagt tggccgacgg
     2041 caatccaatt cttgttctcc tcgcccgcat tcggctggca cgcggaggcc ggaatccacg
     2101 tgctgcggct cgtcctgtcg ggcgcactgg atcgccaccc cggcctcaaa ctcctcagcg
     2161 gtcactgggg cgagttggtg gcggggtggc tcgaccggct cgacgagaca atcggctggg
     2221 ccaagtatct ggaccgcccc gtcagtgact actaccgaca gcacgtgtgg atcacgccat
     2281 caggcatgta cagccagaat cagctgaagt tcttcgtcgc cgagatgggc gccgaccgaa
     2341 tcatccattc cgaggacttc ccgtacgtgg ttcgcgacaa cgtgtcggaa ttcatcgagc
     2401 aggcagacct cagcgacgac gagcgcaccg cgatcgcaca tcgaaacgcg gagaaggtga
     2461 tgcgaatctg acgccagtgc ttgatcctgc cggtggccga ccggctcaga tcggccacca
     2521 atcgcggggt tgacttcgat ggcgggctag gttgacggca atgcctgacc cgacgcccgc
     2581 gcagtctcca cagtcgtcgt tcccgcgcgg catggcgtta ttggtggccg ctgcgttctt
     2641 catggagatc ctcgacgcca cgatcatcgc gccggccata ccgcgcatcg ccgagtcgtt
     2701 cggcgtcgcg gcggtgaaca tcaacgtggc gatctcggcg tacctgatca ccgtcgccgt
     2761 gctgatacct gcgacgggat ggatggccga ccggttcggc gttcggcgcg tgttcgtcac
     2821 cgcgattgcg gtgttcacga tcgcctcagt cggctgcgct gccagtacgt cgcttccaat
     2881 gctggtgggc atgcgcgtgc tgcaaggcgt cggcggcgca atgatggttc ccgtcggacg
     2941 actggccgtg ttgcggcact ccggcaagtc cgatctcgtc cgcgccatcg ccctgctcac
     3001 ctggccggca ctggccgcac cagtgatagc cccggccttg ggcggcgcga tcgccacgtt
     3061 cgtatcgtgg cgctggattt ttttgatcaa cattccgatc ggcgtgatcg gctttctcct
     3121 ggcgctcagg cttgtacatg gtgagccggt gccgtcggcg cgcccactgg actggcgcgg
     3181 aatgctgatg atcgggctgg gaatcgcgtc cgcgctgatc gcgctcgaac agcttcacgt
     3241 gagcggcacc gactgggccc tcgtcggagt gtccggagca gccgcagtgg tgctgctcgg
     3301 ctgtgcggcc aggcacctcc tgcgctcgga tgcgccgctc gtgcaattga gggtgctgcg
     3361 ggaacggaca ctgcggatca ccgtcacggc cggatccatg taccgcatgg tgatcaccgc
     3421 cgtcccgttc ctgctgccgt tgcagttcca gttcgtgttc ggctggacgc cattcagcgc
     3481 gggcctcatg gtcgccgcgt tgttccttgg caacctcacc atcaagccgg cgactactcc
     3541 gctgatgcgc agattcggca tccgtaacgt gctgctcgcc aacggaatgg cgtcggtcgg
     3601 atgtttcgga ttactggcgt tgcttgcgcc gggacttccc attgtcgcga tggccgccat
     3661 tctctatgtc agcggtgcat tgcgctcgat cggcttcacc gcatatgcca gcttggcgta
     3721 ctcgggtatc gagggtgacg acctcaccca cggaaacacc ctgaactcat cggttcagga
     3781 acttgccgcc ggcctcggaa tcgccatagc agcactgctt ctcagcattc tgtcgtcgta
     3841 cccttatgtt tatctggtgc tcggcgcagt gctggccacg acgctgatcg aaacgcttcg
     3901 cctgccccgc gatgccgggt cacatgtcag tggatgacgg ctcaacccgc aggaagcgat
     3961 tcggcgactc gtgacagcgc ggcgaggtgg tcgtcgacgg ttctcaggcc cgcgcccatg
     4021 gtattgaccg atgcgtgcgt cgcacccgct tcggcccatt gcttcaaccg ctcgatgacc
     4081 ccgtcgacgt cgccgcgcca atccacgcga ccctccatgc cgatcgacgc cggatcccgg
     4141 cctgccgcga ccgcggcgcg ttcgaccacg gtgcgcgctt cgtcgagcgc aggccccggc
     4201 cccatcatgg gaaaccagcc gtccgccagg cgcccggcgc gcttgtagcc cggcggcgac
     4261 gccgccccga cccataccgg gatcggacgc tggacgggca ggggcgcgat acccgcgccg
     4321 gtgagcttgt cgaactcccc gtcgaacgtc accgactgct cggtccagag gcgccgcagc
     4381 agggcgatct gctcggcaga ccttttgccg cgactgccga aatcctcgcc gagcgcctcg
     4441 tactcgacgg cgttccagcc gacaccgatg ccgagccgga accggccgcc gctgagcagg
     4501 tcgacctctg cggcctgctt ggcgaccaac gctgtctggc gctgcggcag gatgatgacg
     4561 ccggtgacga gctcgagtga ccgcgtgacc gcggcgagat agccgtacaa gacgaagggc
     4621 tcatggaacg tcgtgtgtac gtcgtacggt ccattccagc cggtatggac cttcgggtcg
     4681 gcgccaacga cgtggtcgta tacgagaatg tgacggtagc cgagttcctc gacgccctcg
     4741 gcgtacgcgc gtacggcgcc cgcatctcca ccgagttcgg tctgcggaaa cgtcactcca
     4801 actcgcatgt cctcgtcctt ccggcacgat ttcctcgaaa gctatgacac cgcgtgtgct
     4861 cggtgcattc ccgggacatc atggctgaat gagcgatgat cgcgattacc cgcagatggc
     4921 cgcctcgcgc ggacggatcg agccggcgcc acggcgggtc cgcggctttc tcggccacga
     4981 gctggtcttc gataccaccc acgcccgcta tgtctgggaa gtgccgtact acccgcagta
     5041 ctacatcccg ctcaccgatg tgcgtacgga gtttctgcgc gacgaggacc acccacagaa
     5101 ggttcagttg ggctcctcgc ggttgcactc actgatcgga gccggccaca cacatgagaa
     5161 ggcggccagg gtgttcgacg acggacccgt cgcgggcctg gtgcgcttcg agtgggaccc
     5221 gctcagctgg ctggaggagg acgaacagat ctacggtcat ccgcgcaatc cctacgcacg
     5281 cgtggacgcg ctccggtcac accgtcatgt ccaagtgcgg ctggacgacg tcgtgctcgc
     5341 cgataccgtc gctccagtcc tgcttttcga aaccggcttg ccgacaaggt attacatcga
     5401 ctattcagat gtcgccttcg agcatctgga gcccagcgac acccagacgc tctgccccta
     5461 caagggcacc acgtcgggct actggtctgt ccggactgct gacacgctgc acccggatct
     5521 ggcctggaca taccactacc cactcccggc ggtggcgccg atcgccggcc tgatggcctt
     5581 ctacaacgag aaactcgacg tcgtcgtcga cggtgtctcg ctggcacggc cgaagacgca
     5641 cttcagctga ctcagccccc gttcaggcgc tcgatctgcc gatgccgcga gacgccgtcc
     5701 tgaatcctga cgacgtgcgc gggctccatc cctgaaggat tacgctcgct tcgtccctcc
     5761 ttggcgatcg gagcatgatg gcgcgaatca caccggccga cggtttcgcc gcgataccga
     5821 ccaccgatat cggtcagggt gatcgcatca acctcggtgt tgcaggcatc atgtcgcgcc
     5881 tgcccgatgt cgccgccgcc gtcggtggcg tcaccggtgc gctgcgcgag gcgggcgccc
     5941 tctcgccgcg gctgatggag cttgtccggc tgcgcatcgc ctttcacaac caatgccgca
     6001 gctgcatgtc gatccgctac cagagcgcga tcgacgacgg cctcaccgag gatgcggtgt
     6061 gctcactcga gcgacccgcc gaggcgtcgg acctgacgga tcccgagcgc agcgcattgc
     6121 ggttcgctga cctgttcgcc accgaccatc tcgcgatcgg cgactcggtg tatgacgacc
     6181 tgcgccggca tttcactgag gaccagttgg tcgccttggg ccttcactgc gcgctgtgcg
     6241 tcgggcttgg gcgcctcgcc gcgacttggc atgtcgtcga cgatctgccc gacgaatatc
     6301 gggccgacag tggaatcccc ctcgcgccgt ggagcgcgaa ttcggttgtc gcgtcgggct
     6361 gaatctttac cttgaaggcg tcaacccaac gggtccgggc taacgctcga tgacggagat
     6421 cgagttggcc ctgaagtcga ccacccatgc ggtgtgcgtg gccgggtcgg cggcgacggt
     6481 ttgcggcccg tcgccgactc cgacgctatc ggtgacgaca cgcttctgag agtcgatcac
     6541 agacaccgat gccgcaccgt ggttggtgac cagaaccgtg tgggcttcgg agtcgaccgt
     6601 gcccgccgac ggctgctgtc ctacggcgat cgtctggatg acggcacggc tgttgaggtc
     6661 taccacagac accgagttgt cgtctgtgtt cggaac
//
LOCUS       DASYLX010000780         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3553254,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000780 DASYLX010000000
VERSION     DASYLX010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3553254"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VGP27_18270"
     CDS             complement(<1..450)
                     /locus_tag="VGP27_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEV7853277.1"
                     /translation="MRLVDLLPVPSLRVQRLIAAAVVLTQGGIAVTGAVVRVTASGLG
                     CPTWPQCFPGSFTPVPHAEVPGIHQAVEFGNRMITFLVVLTAILAVLAVTRARRRREV
                     LVYAWLMPASTVMQAVIGGITVRTGLLWWTVAIHLLVSMAMVWLAVLL"
     gene            complement(639..1412)
                     /locus_tag="VGP27_18275"
     CDS             complement(639..1412)
                     /locus_tag="VGP27_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853278.1"
                     /translation="MTNRFAPGTFTPDPRSATVPTMLAAQFALELKLLLRNGEQLLLT
                     LFIPITLLIGLTLLPFGSFGPNRAATFVPAIMSLAVISTAFTGQAIAVAFDRRYGALK
                     RIGATALPVWGIIAGKSLAVVTVVFLQSIVLGGIGIALGWRPHVVGLMLGATIIALGT
                     AGFTAMGLLLGGTLRAEIVLAVANLLWFVFAGLGALTLEGQVVPRAVQWAARLTPSGA
                     LTEALTQAMSLSVDWFGVAVLAVWGAVSALCAMRWFRFT"
     gene            complement(1409..2362)
                     /locus_tag="VGP27_18280"
     CDS             complement(1409..2362)
                     /locus_tag="VGP27_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853279.1"
                     /translation="MTSGGPLVERSSAVPVRLRGVRKRYGSTTAVSGLDLDVQSAQVL
                     ALLGPNGAGKTTTVEMCEGFIKPDAGTIEILGLNPITDNARVRERIGVMLQGGGAYPA
                     AQAGEMLNLVAAYSANPLDPQWLMETLGLTEAAKTTYRRLSGGQQQRLALACAVVGRP
                     ELVFLDEPTAGMDAHARIVVWELIDALRSDGVTVVLTTHQLTEAEELADRLVIIDHGV
                     AVASGTPAELMRSGAENQLRFKAPRMLDLTLLVSALPESYRATEASPGEYLVEGHIDP
                     QVLATVTAWCARLNVLATDMRVEQRSLEDVFLDLTGRELRS"
     gene            complement(2359..>3466)
                     /gene="mptB"
                     /locus_tag="VGP27_18285"
     CDS             complement(2359..>3466)
                     /gene="mptB"
                     /locus_tag="VGP27_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEV7853280.1"
                     /translation="LCHRLMVLLGVALIVWAVPRLARRCGVAEVSALWLGAANPLLIM
                     HLVAGIHNEALMLGLMLAGTEFALRGVYADRPLLPKPLAWPQSRDDWIRWRPMATLVF
                     GVVLITLSSQVKLPGLLAVGFIAMALACRWGGTFKAFLIAGGSLAAVSFAVMAVIGWA
                     SGLGFGWLFTLGTANVVRSWMSLPTLLALGTGQVGILLGLGDHTTAVLGLTRGIGVVI
                     IAILVTWLLLAVLRGRLHPVGGLGVALGMTVLLFPVVQPWYVLWAVIPLAAWATRRSF
                     RTTAIVVTLVVGIFGPTANGDRFTVFQILLATAASTVIVALLIAITYYRLPWRKLPDP
                     GPELDIVPLPEPVAAAASLPQPQAAKADAYAESP"
BASE COUNT          667 a         1214 c         1121 g          464 t
ORIGIN      
        1 cagcagcacg gccagccaca ccatggccat cgagaccaac aggtgaatcg ccactgtcca
       61 ccacagcagg ccggtccgca ccgtgatgcc gccgatgacc gcctgcatca ccgtcgacgc
      121 gggcatcagc caggcataga ccagaacctc gcggcgacgg cgggcgcggg tgaccgccag
      181 caccgcaagg atcgcggtca gcacgacgag gaaggtgatc atccggttgc cgaactcgac
      241 ggcctgatgg atgccgggca cttcggcgtg cggcacggga gtgaaactgc cggggaagca
      301 ctgcggccat gtggggcagc cgaggcccga tgcggtgacc cggacgacgg cgcccgtgac
      361 ggcgatgccg ccctgcgtca ggacgacggc ggcggcgatc aggcgctgca cgcgcaggct
      421 cggcaccggc agcaggtcca ccaagcgcat caagaatcgt ccgacggcca cggcccgatc
      481 gtagaggatc gcaactacag ctcgtagtag ccagcgtcag gtgaagggtt cggatattgc
      541 acacagggtc gtggttcgag ccgcgccagc gaccctcagt gcaatcttca ggagcagatt
      601 gcaaacagat gccgatggat tggccgtgtt cgtttgcgtc aggtgaagcg gaaccagcgc
      661 atcgcacaca acgctgacac cgcgccccag accgcgagca cggcgacgcc gaaccagtcg
      721 accgacaacg acatcgcctg tgtcagcgcc tccgtcagcg cacccgaggg cgtgagccgc
      781 gccgcccact gcactgcccg gggaacgacc tgtccttcga gggtgagtgc gcccagcccg
      841 gcgaacacga accacagcag gttggcgacg gccagcacga tctcggcgcg cagcgtgccg
      901 ccgagcaaca ggcccatcgc cgtgaaccct gcggtgccga gcgcgatgat ggtggcgccg
      961 agcatcagcc caacgacgtg tggccgccag cccagcgcaa tgccgatgcc gcccaacacg
     1021 atcgactgca ggaaaaccac ggtgacgacg gccagcgact tgcccgcgat gatgccccag
     1081 accggcaatg ccgtcgcgcc gatccgcttg agggcgccgt agcggcggtc gaacgccacg
     1141 gcgatcgcct ggccggtgaa cgccgtcgag atcactgcca gcgacatgat tgcgggaacg
     1201 aaggtggcgg cccggttcgg cccgaacgag ccgaacggca gcagtgtcaa cccgatcaac
     1261 aacgtgatcg ggatgaagag ggtgagcagt agctgttcgc cgtttcgcaa caacagcttc
     1321 agctcgagcg cgaattgcgc ggccagcatc gtcggcaccg tcgccgaacg cggatcgggc
     1381 gtgaacgttc caggcgcgaa gcgattggtc atgaccgcaa ctcccgcccg gtcaggtcca
     1441 ggaacacatc ttcaagactg cgctgctcca cccgcatgtc ggtggccagc acattgagcc
     1501 gggcgcacca tgccgtcacc gtcgcgagca cctgtgggtc gatgtgccct tccaccaggt
     1561 attcaccggg ggaggcttcg gtggcccggt agctttcggg cagtgccgag accagcagcg
     1621 tcaggtcgag cattcgcggg gccttgaacc tcaattggtt ctccgcgccg ctgcgcatca
     1681 gttcggcggg tgtaccgctg gccaccgcga ccccgtggtc gatgatcacc agccggtctg
     1741 ccagctcctc cgcttcggtc agctgatggg tggtcagcac gaccgtcaca ccgtcgctgc
     1801 gcagcgcatc gatcaactcc cacaccacta ttcgagcgtg tgcgtccatg cctgcggtcg
     1861 gctcgtcgag gaataccagt tccgggcggc cgaccaccgc gcacgccaat gcaaggcgct
     1921 gctgctggcc gccggacagc ctgcgatacg tcgtcttcgc cgcctcggtc aggcccagcg
     1981 tctccatcag ccactgcggg tccaacgggt tggcactata agccgcaacc agattgagca
     2041 tctcgcctgc ctgggccgcc ggatacgcgc cgccgccctg cagcatgacc ccgatgcgct
     2101 cacgcacccg cgcattgtcg gtgatcgggt tcaggccgag gatctcgatc gtccccgcgt
     2161 ccggcttgat gaagccctca cacatctcca ccgtggtcgt cttgcccgcc ccgttgggcc
     2221 cgagcagcgc cagcacctgt gcggattgca cgtcgaggtc gaggccggag acggccgtcg
     2281 tcgacccgta ccgtttgcgc actccgcgca ggcgtaccgg gactgccgac gagcgctcga
     2341 ctagtgggcc gccggaggtc acggagattc agcgtaggcg tccgccttcg ccgcctgtgg
     2401 ctgcggtagc gaggctgcgg cggcgaccgg ctccggcagt ggaacgatgt cgagctccgg
     2461 gccggggtcc ggcagcttcc gccagggcag ccggtagtac gtgatcgcga tgagcagcgc
     2521 gacgatcacg gtgctggccg cggtggcaag cagaatttgg aacaccgtga atcgatcacc
     2581 gttggccgtc gggccgaaga tgcccaccac gagcgtgacg acgatcgccg tggtgcggaa
     2641 cgaacgcctg gtcgcccatg ccgcaagcgg gatcaccgcc cacagcacgt accagggctg
     2701 gacaacaggg aagagcagca ccgtcatgcc gagcgcaacg ccgaggccac cgaccgggtg
     2761 caggcgaccg cgaagcaccg ccaacagcag ccacgtcacc aggatggcaa tgatgaccac
     2821 gccgatgccg cgggtcagcc cgagtaccgc agtggtgtgg tcgcccagcc cgagcaggat
     2881 gccgacctgt ccggtaccca gcgccaacaa tgtcggcaac gacatccaac tgcgcaccac
     2941 gttcgccgtg cccagggtga acagccagcc gaacccgagc ccgctggccc agccgataac
     3001 cgccatcacc gcgaacgaca ccgcggccag cgaaccgcct gcgatcaaaa acgccttgaa
     3061 ggtgccgccc caccggcagg ccaatgccat cgcgatgaag ccgacagcca gcagcccagg
     3121 cagcttgacc tgtgaggaca gggtgatcag cacgacacca aacaccagcg tcgccatcgg
     3181 tcgccagcga atccagtcgt cacgagactg cggccacgcc aagggcttcg gcagcaacgg
     3241 cctgtcggcg tacacgccgc gcagggcgaa ctccgtgccc gcgagcatga gaccgagcat
     3301 cagcgcctcg ttgtggatgc ccgccaccag atgcatgatc agcagcggat tagccgcccc
     3361 cagccacagc gcgctgacct cggcgacgcc gcaccggcgc gcgaggcgcg gcaccgccca
     3421 cacgatgagg gcgacaccga gcagcaccat caaccggtgg cacagc
//
LOCUS       DASYLX010000781         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_591962,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000781 DASYLX010000000
VERSION     DASYLX010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591962"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VGP27_18290"
     CDS             <1..1017
                     /locus_tag="VGP27_18290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV7853281.1"
                     /translation="FNETVYCLGVEKLLGVTDDIPERASIIRVMMMEMNRISSHLVAL
                     ATGGMELGSMGAMFYGFREREQILSIFETITGLRMNNSYIRPGGLAADLPDEALPQIR
                     DMLKLMPKRLKDLSDLLSENYIWKARTQGIGYLDLTGCMALGITGPVLRSTGLPHDLR
                     RAQPYCGYETYDFDVITDDGCDAYGRYLIRVKELHESLKIIEQCVERLEKTPGRVMID
                     DKKLAWPADLKLGPDGLGNSPEHIAKIMGHSMEGLIHHFKLVTEGIRVPPGQVYVAVE
                     SPRGELGVHMVSDGGTRPYRAHYRDPSFTNLQAVAAMCEGGMVADAISAVASLDPVMG
                     GVDR"
     gene            1046..1942
                     /gene="nuoE"
                     /locus_tag="VGP27_18295"
     CDS             1046..1942
                     /gene="nuoE"
                     /locus_tag="VGP27_18295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEV7853282.1"
                     /translation="MSLTHNGSPVFIELGQRPDEAGPPIAGESLYPADVTARLETSAK
                     DIIARYPRSRSALLPLLHLVQSEDGYLTKAGLAFCAAQLDLTEAEVTAVATFYSMYRR
                     TPTGEYLVGVCTNTLCAIMGGDAILDSLQDHLGVHAGETTEDGRVTLEHIECNAACDY
                     APVIMVNWEFFDNQTPSSARDLVDSLRAGEQVVPSRGAALCSFRQTARILAGLPDDRP
                     VTADGQVATATLAGLLVARELDMEAPSPDSEATAPPPKSDGTKAEAVETTKDEPAPGP
                     SATVPAEPATSETDPSKTDSHD"
     gene            1944..3269
                     /gene="nuoF"
                     /locus_tag="VGP27_18300"
     CDS             1944..3269
                     /gene="nuoF"
                     /locus_tag="VGP27_18300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV7853283.1"
                     /translation="MAPATPLTPVFSRYWDEPEPWSLDTYRRHDGYRALDKALAMDPD
                     EVIALVKDSGLRGRGGAGFPTGTKWSFIPQDDTGAGAKPHYLVINADESEPGTCKDIP
                     LILTTPHFLVEGAIIAAYAIRAHHAFIYVRGEVVPVLRRLQVAVAEAYEAGYLGTDIN
                     GSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYACPTVVNNV
                     ESIASVPPIMLNGIDWFRSMGSEKSPGFTLYSLSGHVTNPGQYEAPLGITLRELLEYA
                     GGVRAGHELKFWTPGGSSTPLLTAEHLDVPLDYEGVAGVGSMLGTKALQIFDETTCVV
                     RAVRRWTEFYAHESCGKCTPCREGTYWLSQIYERLETGRGTEEDIGKLLDISDTIFGK
                     SFCALGDGAASPILSSIKFFRDEYVEHLDGGCPFDPHASTLMATEGVGA"
     gene            3269..>5212
                     /locus_tag="VGP27_18305"
     CDS             3269..>5212
                     /locus_tag="VGP27_18305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEV7853284.1"
                     /translation="MTVTEPSQGAPAVEMVNLTIDDVPISVPKGTLVIRAAELIGVQI
                     PRFCDHPLLDPVGACRQCLVEVEGQRKPMASCTITCTPDMVVRTQMTSESADKAQHGV
                     MELLLINHPLDCPVCDKGGECPLQNQAMSNGRAETRFEDVKRTFPKPIPISSEVLLDR
                     ERCVLCARCTRFSQQIAGDPFIELLERGALQQVGIAPGEPFSSYFSGNTVQICPVGAL
                     TNTAYRFRARPFDLVSSPSACEHCASGCAQRTDHRRGKVLRRLAGDDPEVNEEWNCDK
                     GRWAFNYAGVGDRITTPLIREDDGKLRSASWSEALGVAASGLAAAAGRAGVLVGGRST
                     VEDAYAYSKFARMVLGTNDIDFRIRAHSQEEADFLASNVAGQPMTVTYADLEKAPAVL
                     LVGFEPEDESPIVFLRLRKAARENGLQVLAIAPFATRGLTKMSGTLIRTAPGGEAAAL
                     DELAEDALLNQPGAIILVGERLATSPGALSAASRLATATGARLGWVPRRAGDRGALEA
                     GALPNLLPGGRPVADASAREQTAIAWHISELPGTPGRDTAGILAAAADRQLDALLIGG
                     VELADLPDPDAALAAIDAVPFVVSLELRESAVTDLADVVFPVAPVVEKAGSFINWEGR
                     IRPFQPSLPSNATPDLRVLYTLAD"
BASE COUNT          882 a         1764 c         1756 g          810 t
ORIGIN      
        1 ttcaacgaga cggtgtactg cctcggcgtc gagaagctgc tcggggtcac cgacgacatc
       61 ccggaacgcg ccagcatcat ccgggtgatg atgatggaga tgaatcgcat ctcgtcgcac
      121 ctggtggcgc tggccaccgg cgggatggag ctgggctcca tgggcgcgat gttctacggc
      181 ttccgcgaac gcgagcagat tctgtcgatc ttcgagacca tcaccgggct gcggatgaac
      241 aactcctaca tccggcccgg cgggctggcc gccgacctgc ccgacgaggc ccttccgcag
      301 atccgcgaca tgctcaagct gatgccgaag cggctcaagg acctctcaga cctgctgagc
      361 gagaactaca tctggaaggc ccgtacccag ggcatcggct acttggatct gacggggtgc
      421 atggcactgg gcatcaccgg cccggtgctg cgctccaccg ggctccccca cgatctgcgc
      481 agggcgcagc cgtactgcgg gtatgaaacc tacgacttcg acgtcatcac cgacgacggg
      541 tgtgacgcct acggccgata cctgatccgg gtcaaggagt tgcacgagtc gctgaagatc
      601 atcgagcaat gcgtcgagcg tttggagaag acgccgggcc gggtgatgat cgacgacaag
      661 aagcttgcct ggccggccga tctgaagctg ggtcccgacg ggctcggcaa ctcgcctgag
      721 cacatcgcga agatcatggg ccactcgatg gagggcctga tccaccactt caagctggtg
      781 accgagggca tccgggtgcc accgggacag gtctacgtcg cggtggagtc gcccaggggc
      841 gaactcggcg ttcacatggt ctccgacggc ggcacaaggc cctaccgggc gcactaccgc
      901 gacccctcgt tcacgaacct gcaggcggtc gccgccatgt gcgagggcgg catggtcgcc
      961 gacgcgatct ccgcggtcgc gtcgctcgat ccggtgatgg gcggggtgga ccgatgagcg
     1021 cttgcgcgaa gagacgagag gacagatgag cttgacgcac aacggttctc cggtgttcat
     1081 cgagctgggt cagcgccccg acgaggccgg tccgccgatc gcaggcgaat cgctgtaccc
     1141 ggccgacgtg acggcccggc tcgaaaccag cgcgaaggac atcatcgccc ggtacccgcg
     1201 gtcgcggtcg gcgctgctgc cgctgctgca cctggtgcag tccgaggacg gctacctcac
     1261 caaggcgggc ctcgcgttct gcgccgccca actcgacctg accgaagccg aggtgaccgc
     1321 ggtcgccacg ttctattcga tgtaccggcg caccccgacc ggcgagtatc tggtcggcgt
     1381 ctgcaccaac acgctctgcg cgatcatggg cggcgacgcc atcctcgatt ctctgcagga
     1441 ccacctgggc gtgcacgcag gcgagaccac cgaagacggt cgcgtcaccc tcgaacacat
     1501 cgagtgcaac gcggcgtgcg actacgcacc ggtcatcatg gtcaattggg agttcttcga
     1561 caaccaaacg ccgtcgtcgg caagggacct ggtcgattca ctgcgcgcgg gtgaacaggt
     1621 cgtgcccagc cgcggcgcgg cgttgtgcag ttttcgccaa accgcccgca tcctcgcagg
     1681 gctgcccgac gatagaccgg tcaccgcgga cgggcaggtg gcgacggcca cgctggccgg
     1741 gctcctggtc gcccgcgaac tcgacatgga agcgccgtcg cctgacagcg aggccaccgc
     1801 gccgccgccg aagtccgacg gcacgaaggc ggaagccgtc gagaccacga aagacgagcc
     1861 ggcgcctgga ccgtcggcca ctgtgccggc cgaaccggcc acctccgaaa ccgacccgag
     1921 caaaacggat tcacacgact gacatggcac ctgctactcc cctcacaccc gtattcagcc
     1981 ggtactggga cgaacccgaa ccctggtcgc tggacactta ccgccgccac gacggatacc
     2041 gcgcactgga caaggcgctg gcgatggatc ccgacgaggt catcgcgctg gtaaaggact
     2101 ccggactgcg tggtcgcggc ggcgccggct tcccgacggg caccaagtgg tccttcatcc
     2161 cgcaggacga caccggcgcc ggcgcgaagc cgcactatct cgtcatcaac gccgacgagt
     2221 cggaacccgg cacgtgcaag gacattccgc tgatcctgac cacaccgcac ttcctcgtcg
     2281 agggcgcgat catcgctgcc tatgcgatcc gcgcccacca cgcgttcatc tacgtccgcg
     2341 gtgaggtggt gccggtgctg cggcggctgc aggtcgccgt cgcggaggca tacgaagccg
     2401 gctacctcgg aacggatatc aacggctcgg gcttcgacct cgaactggtg gtgcacgcgg
     2461 gcgcgggtgc ctacatctgc ggcgaagaga ccgcgctgct cgactcgctc gagggcaggc
     2521 gcggccagcc ccgacttcgg ccaccgttcc ccgcggtggc cggcttgtac gcgtgcccga
     2581 cggtcgtcaa caacgtcgag tccattgcca gcgtgccgcc gatcatgctc aacggcatcg
     2641 actggtttcg gtcgatgggc tcggagaagt cgcccggctt cacgctctac tcgctgtcgg
     2701 ggcatgtcac caaccccggg caatacgagg cgccgctggg catcactctg cgcgaactgc
     2761 tcgagtacgc gggcggcgtc cgcgcaggcc atgagctgaa gttctggacg cccggcggct
     2821 cgtcgacccc gctgctgacc gccgaacacc tcgatgtgcc actggattac gagggagtgg
     2881 caggtgtcgg gtcgatgctg ggcaccaagg cgctgcagat cttcgacgag accacctgcg
     2941 tggtgcgcgc ggtgcggcgc tggacggagt tctacgccca tgagtcgtgc ggcaagtgca
     3001 caccgtgtcg tgagggcacc tactggctga gccagatcta cgagcgactc gaaaccgggc
     3061 gcggcaccga agaggacatc ggcaagctgc tggacatctc cgacacgatc ttcggaaagt
     3121 cgttctgcgc gctgggcgac ggtgcggcaa gcccgatcct gtcgtcgatc aagttcttcc
     3181 gcgacgagta tgtcgagcat ctggacggcg gctgcccgtt cgatccgcac gcctcgaccc
     3241 tgatggcgac cgagggggtg ggtgcctgat gacggtgacc gagccgagcc agggtgcgcc
     3301 tgcagtggag atggtgaacc tgaccatcga cgacgtgccg atcagtgtgc ccaagggcac
     3361 gttggtgatt cgcgcggccg agcttatcgg cgtccagatc ccgcggttct gcgaccaccc
     3421 gttgctcgat ccggtgggcg cgtgcaggca gtgcctcgtg gaggtcgagg gccagcgcaa
     3481 gccgatggcg tcctgcacca tcacctgcac ccccgacatg gtcgtccgta cgcagatgac
     3541 ctcggagtcg gccgacaagg ctcagcacgg cgtgatggaa ctgctgctca tcaaccaccc
     3601 gctggactgc ccggtctgcg acaagggcgg cgagtgcccg ctgcagaacc aggcgatgtc
     3661 gaacggccgg gcggagaccc gcttcgaaga cgtcaaacgc accttcccca aaccgattcc
     3721 catctcgtcg gaggtgctgc tggaccgtga gcggtgcgtg ttgtgtgcgc gctgcacacg
     3781 gttctcccag cagatcgcgg gcgatccgtt catcgagctg ttggagcgcg gcgcgctgca
     3841 gcaggtgggc atcgcaccgg gtgagccgtt ctcgtcgtac ttctccggca acaccgtgca
     3901 gatctgcccg gtgggcgcac tgaccaacac ggcataccgg ttccgggcgc ggccgttcga
     3961 cctggtatcc agccccagcg cctgtgagca ctgcgcatcg ggatgcgctc agcgcaccga
     4021 ccatcggcgc ggaaaggtgt tgcgccggtt ggccggcgac gaccccgagg tcaacgagga
     4081 gtggaactgc gacaagggcc gatgggcgtt caactacgca ggagtcggcg accgcatcac
     4141 caccccgctg atccgcgaag acgacggcaa gcttcggtcc gcgtcgtggt cggaggcgct
     4201 cggcgtggcg gcgtccggtc tggccgcggc ggcagggcgg gcaggcgtgc tggtgggcgg
     4261 cagaagcacc gtcgaggatg cctacgccta ctcgaagttc gcccggatgg tgttgggcac
     4321 caatgacatc gacttccgga ttcgcgcgca cagccaggag gaggccgact tcctggcgtc
     4381 caacgtcgcg gggcagccga tgacggtcac ctacgccgat ctggagaagg cgcccgcggt
     4441 gctgctcgtc ggcttcgagc cggaggacga gtcgcccatc gtcttcctgc ggctgcgcaa
     4501 ggccgcccgc gagaacggtc tgcaggtgct ggccatcgcc ccgttcgcca cccggggcct
     4561 taccaagatg tcgggcacgc tcatccgcac cgcgcccggc ggcgaggcag cagcactcga
     4621 cgaactcgca gaagacgcgt tgctgaacca gccaggcgcg atcatcctgg tcggtgagcg
     4681 tctggcgacc tctcccggag ccctctccgc ggcaagcaga ttagccacgg cgaccggtgc
     4741 gcggctcggt tgggtgccgc gccgtgccgg tgaccgcggc gctctggagg ccggtgcgct
     4801 gccgaacctg ctgcccggcg gccgacccgt cgccgacgca tcggcgcggg agcagaccgc
     4861 catcgcgtgg cacatctccg agctgccggg caccccgggc cgcgacacgg cgggcatcct
     4921 ggccgcggcg gcggacaggc agttggacgc tctcctgatt ggcggggtcg agctcgccga
     4981 cctgcccgat cccgacgcgg ccttggccgc catcgacgcg gtacccttcg tcgtcagcct
     5041 ggagctgcgg gaaagcgcag tgaccgacct ggccgacgtg gtcttcccgg tcgcgccggt
     5101 cgtcgagaag gccggctcgt tcatcaactg ggagggcaga attcggccgt tccagccgtc
     5161 gctgcccagc aacgcgacgc ccgatctccg ggtgctgtac acgctggccg ac
//
LOCUS       DASYLX010000782         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2785045,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000782 DASYLX010000000
VERSION     DASYLX010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785045"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGP27_18310"
     CDS             complement(<1..120)
                     /locus_tag="VGP27_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853285.1"
                     /translation="MARIDLFAKIPLIGMSMVFLAMAIVAVTAYLHVGWWSIIG"
     gene            239..688
                     /locus_tag="VGP27_18315"
     CDS             239..688
                     /locus_tag="VGP27_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7853286.1"
                     /translation="MRGAVTIHMNAPAQRVWDLVADIRNTGRFSPETFDAEWLDGATE
                     PSVGARFRGHVRRNEIGPVYWTTCRVTACEPGREFGFEVLLGNRSVNNWRYEFAPNGD
                     GTDVTESFWAPVPAFLRPTSFLGFLRRRRNVRDMRTTLERIKVVVEA"
     gene            complement(788..>1655)
                     /locus_tag="VGP27_18320"
     CDS             complement(788..>1655)
                     /locus_tag="VGP27_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV7853287.1"
                     /translation="SATADAADRLALRIDEMRAGAAGAADAVAWARSRLRDIINRFEE
                     RATAMEARLDEPGTVEELRDEARRALEEAIAVVDQLRSELDGHAASVAAPTNPAGASG
                     APAAPAGFTAPAGSGASPSGMPPGGAALSGPTFGGAVPTDAELAALPLPDPEQFGDGI
                     AVSLPDGSTVTAPNATAANAVRYALTQLGVPYVWGGTTPGVGLDCSGLTQWAYHQAGL
                     DLPRLAQEQDLGAAVNQNSLRPGDLAVWDGHVAMVVGDGQMIEAGDPVHLSPIRTTNA
                     GQGFQGFWRPTA"
BASE COUNT          270 a          564 c          534 g          287 t
ORIGIN      
        1 gccgatgatc gaccaccagc cgacatgtag ataagcggtc accgcgacga tggccatggc
       61 caggaacacc atggacatcc cgatcagtgg gatcttggcg aaaaggtcga tacgtgccat
      121 gggactcacc cccaaaaccg acgtgaactt ctggtgaatc gagtagacac ccgattggtc
      181 ggcgaggcga ctcgaatgga aaagactctc aagtgggcgt gacacgtatc atctggcgat
      241 gcgcggagcg gtgacgattc atatgaacgc gcctgcgcaa cgggtttggg atctggtggc
      301 cgacatccgg aacaccgggc ggttctcacc ggagaccttc gacgcggagt ggctggacgg
      361 cgccaccgag ccttctgtcg gcgcgcggtt ccgaggccac gtgaggcgca acgagatcgg
      421 gccggtctat tggacgacgt gccgggtcac cgcatgcgaa ccgggccgcg agttcggttt
      481 cgaggtcctg ctcgggaacc gctccgtcaa taactggcgc tacgagttcg cgcccaacgg
      541 cgatggcacc gacgtgaccg aatcgttctg ggccccggtt ccggcgttcc tccgtccgac
      601 gtcgttcctc ggcttcttgc gcaggcgaag gaacgtgcgt gacatgcgca ccaccctcga
      661 acgcatcaag gtcgtcgtcg aagcctgagg ccctgcctcc gtcggcgacc atggctgcca
      721 ggaattcttt aagccagtgc ccggattctg gtctcgcccg ctaggccagc tcaccgatta
      781 ggggcgatta tgccgtcggc cgccagaagc cctgaaaacc ctgtcccgcg ttggtggttc
      841 ggattggtga caagtgcacg ggatcacccg cttcgatcat ctgaccgtcg ccgaccacca
      901 tcgcgacgtg tccgtcccat accgccaaat cgcctggccg caatgagttc tgattcaccg
      961 ctgcaccgag gtcctgctcc tgtgcgagcc tcggaagatc cagtccggcc tggtgatacg
     1021 cccattgcgt cagcccgctg cagtcgagcc cgacaccggg cgtcgtgcca ccccagacgt
     1081 agggaacgcc gagctgtgtc agcgcgtacc gcaccgcgtt ggctgccgtc gcgttgggtg
     1141 ccgtcaccgt acttccgtcg ggcaggctga cggctatacc gtcgccgaac tgttctggat
     1201 ccggcaacgg aagtgcggcc agttccgcgt cggtcgggac ggcgccgccg aacgtcggcc
     1261 ctgacagtgc ggccccgccc ggcggcatcc ccgaaggact tgcaccggag ccggcgggcg
     1321 cggtgaaacc cgccggggcg gccggggccc ccgatgcacc agccgggttc gtcggtgccg
     1381 cgacgcttgc ggcgtgcccg tcgagttctg agcgcaactg gtcgacgacg gcgatggcct
     1441 cttccagcgc ccgccgggcc tcgtcccgca gttcctccac cgttcccggt tcgtcgagcc
     1501 gcgcctccat ggcggtcgcg cgttcctcga accggttgat gatgtcccgc agccgcgagc
     1561 gggcccatgc cacggcatcc gcggcaccgg cagcgcctgc ccgcatttcg tcgatccgca
     1621 gcgccaatct gtcggctgcg tctgccgtgg cggac
//
LOCUS       DASYLX010000783         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3116839,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000783 DASYLX010000000
VERSION     DASYLX010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3116839"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGP27_18325"
     CDS             complement(<1..253)
                     /locus_tag="VGP27_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853288.1"
                     /translation="MTLTAEQTSARGLRESLPGLLSLALASCVAVTTEMLPVGLLPEI
                     GTTFAVADSVTGLLVSLYAVMVATLAVPLTVATSRFARKP"
     gene            307..1500
                     /locus_tag="VGP27_18330"
     CDS             307..1500
                     /locus_tag="VGP27_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV7853289.1"
                     /translation="MAQDQRQAGQVRWLGAAQLVDLVRSEPGVTRVAAAQRLGIGSGG
                     ATELVARMRQARLLDQTPAPAQGRGRPTTVLGAHAQGPLVLAADLRATDWRLALAGID
                     GVPDVVAQGRYAGGQLDPALNQLAEEIGRVYRRNSRRLRAIAVSVAGTISGAKLVQFT
                     SRGWHDVDLSVLAAKIPDRAGVSLLLGNDATLAGLAEARSGAARSAATALYLIVAQGI
                     GGVLVVNGEPLTGAYGAAGEYGHIPFGDPKLLCPCGARGCWDLTVDGRALARHLGEAP
                     PDDPVGYVHDLIGRPKRDAATRRAFEAVAASIGRGVSALVNLHDPEVVTLGGVAAQLR
                     AAAPTVFDDALRDGLMSFRKGSPPPVRDAEHGEDGPLHGGVAMALDHITTAAGLADWV
                     GRQRG"
     gene            complement(1508..>2087)
                     /locus_tag="VGP27_18335"
     CDS             complement(1508..>2087)
                     /locus_tag="VGP27_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV7853290.1"
                     /translation="VTEVPFDPDASGVALLLIDSRAPHRHAGGEYAARRRSCERAAAA
                     MGVGSLRDAQDWGMAALDSVKDQVDARRARHIFTENARVLDFVAALKEREWDRAGSIL
                     TASHTSMRDDFEITTPQIDLIADTAVGAGALGARMTGGGFGGCVIALVPEDALDAIMT
                     AIPGACREAGFNQPTLVRATAASGAGDVLPES"
BASE COUNT          333 a          711 c          715 g          328 t
ORIGIN      
        1 gaggctttct ggcgaaccga gacgtcgcaa cggtcaaggg gaccgcaagc gttgcgacca
       61 tgaccgcata gaggctgacc aacagtccgg tgaccgagtc tgccaccgcg aacgtcgtgc
      121 cgatctccgg tagcaagccg acaggcaaca tctcggtcgt tacggctacg cacgatgcga
      181 gcgccagcga cagcagtccc ggcaggctct cccgcaggcc ccgtgctgat gtctgctcag
      241 ccgtcaacgt catctccgtc gaatatactc gtggaccgag tttattcaag tgggatacta
      301 ccggcgatgg cacaggatca acggcaagcc ggacaggtgc gctggttggg cgccgctcaa
      361 ctggtcgatc tggtgcgatc ggagcccggc gtcactcgcg tcgccgctgc gcagcgcctc
      421 ggcatcggca gcggcggcgc caccgagttg gttgcgcgga tgcggcaggc ccgactgctc
      481 gaccagacac cggcccccgc gcaggggcga ggcaggccga cgaccgtatt gggtgcgcac
      541 gcgcagggcc cgctggtgct cgccgccgac ttgcgcgcaa cggattggcg cctcgcactg
      601 gcgggaatcg acggcgtgcc cgacgttgtg gcccagggcc ggtatgcggg tggacagctg
      661 gatcccgcgc tcaatcagct cgcagaggag atcggaaggg tctatcgacg aaactcccgg
      721 cgcctgaggg caatcgcggt gtcggtcgcg ggaaccatca gcggcgccaa gctcgttcag
      781 ttcacctccc gcggttggca tgacgtcgac ctgtcggtgc tggccgcgaa gatcccggac
      841 cgggcgggcg tttcgctgct gctcggtaac gacgccacgt tggccgggct ggccgaggcg
      901 cggtccggcg ccgcccgctc agccgccact gccttgtatc tgattgtcgc ccaagggatc
      961 ggcggcgtgc tagtggtgaa tggggagccc ctcacaggcg catatggcgc agcgggcgag
     1021 tacggtcaca tccccttcgg cgacccgaag ctgctatgcc cgtgcggcgc acgcggctgc
     1081 tgggatctca cggtcgacgg tcgtgcgttg gcccggcatc tcggcgaggc accccccgac
     1141 gatccggtcg gctatgtcca cgacctgatc ggccgcccca agcgcgacgc cgcaacacgg
     1201 agggcattcg aagccgtggc ggcctcgatt ggccgcggtg tcagcgctct ggtgaacctt
     1261 catgaccccg aggtcgtcac gctgggcggc gtcgcggcgc agctgcgggc tgccgccccc
     1321 accgtcttcg acgatgcact cagggacggc ttgatgtcct tccgtaaggg gtctccgcca
     1381 ccggtgcgcg acgccgagca cggcgaggac ggaccgctgc acggtggcgt cgcgatggcg
     1441 ctggaccaca tcaccaccgc agccggcctg gcggactggg tcgggcgtca gcgtggctga
     1501 ccgtcgatca cgactcgggt aaaacgtcac cggcacctga agcagccgtt gcccgcacca
     1561 gcgtcggttg gttgaagccg gcctcccgac aggcaccggg gatcgcggtc atgatggcgt
     1621 ccagagcgtc ttcgggtacg agcgcgatga cacaaccgcc gaatccgccg ccggtcatcc
     1681 gcgcgcccaa tgcgcctgcg ccgacggcgg tgtcggcgat gaggtcgatt tggggagtgg
     1741 tgatctcgaa gtcgtcacgc atcgacgtgt gcgacgcggt caggatcgat cccgcccggt
     1801 cccactccct ttccttcagc gcggcgacga aatcgagaac tcgggcattc tcagtgaaaa
     1861 tgtggcgggc ccgtcgcgca tcgacttgat ctttcacact gtccaacgcc gccatccccc
     1921 agtcctgcgc atcgcgcagt gagcccacgc ccatggcggc cgcggcccgt tcgcacgaac
     1981 gcctccgcgc cgcgtactca ccaccggcgt gccgatgcgg cgcgcgtgag tcgatcagca
     2041 gcagggcgac accggaggcg tcggggtcga acggcacctc ggtgacg
//
LOCUS       DASYLX010000784         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_482178,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000784 DASYLX010000000
VERSION     DASYLX010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482178"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..890
                     /locus_tag="VGP27_18340"
     CDS             609..890
                     /locus_tag="VGP27_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853291.1"
                     /translation="MDALDVFGIFDTACRGDTPSAKASLMPWRDLPTHLRQSNNAQVG
                     RTANLWSESRKKEIDTVEHLPALLAGADLYLRRLSATEHWPTRQMRQQC"
     gene            939..2021
                     /locus_tag="VGP27_18345"
     CDS             939..2021
                     /locus_tag="VGP27_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV7853292.1"
                     /translation="MTTTITVLEGDQTGQELLECALRVLDPATCGVELNLQRFDLSLS
                     NRRATNNTVVFAAADAMKECGLGLKAATITPEVAGDVGSPNAILRERVNGKVIVRTGR
                     RIPGVASIAGVSAPISVIRMAVDDAYGAKEWREGAGDDEVAYRTEKITRSTCRAVAEF
                     AFVQARRTRARVFGGPKYTVSPVYEGMLKEEMDTAAGRHSDVPYEPQLIDATYALLLT
                     QGGDPLVIPALNRDGDCLSDFVLQLFGSIAGAESILIGFQGNSTIPEVVMAEAPHGTA
                     PALEGKNIANPMAMMLACASLLGFVREPSAKRASRAIYEAIFETVRSGTKTADLGGHC
                     LTDEFTDTVIDGVRAKLEIWESIGVD"
BASE COUNT          387 a          647 c          659 g          360 t
ORIGIN      
        1 gcttgtccga ggggggacgt ggacactaac cacacgcctg gcgctcggta tctccggagc
       61 atgaagcgtt gtcacgtttc atccctagcc cggtgcggca gcgctggggc cagtgtgtat
      121 agcgttcttg ctatgccccg gcgtaggatg acacagtctg ctgtggcgcg tttcgaggcc
      181 ggatgcacga ttccgacatt gccagtcctg ggaaggatcg cacaggcctt ggatgcggac
      241 ctgaccgtcc aggtttcggc tcgaaccggc gcggcctgac cgacggtaac gtatcggccc
      301 gttcggccga accctggtgc gtgcgctcgt gcgccaacct cacagcgagg gctatcaccg
      361 ccgcaccgtt cttgtccgtg gcgaaccgcc gccgtagccg atactgcccg gcgcatcgac
      421 gcggcgggtc gcgggtgcac agctacgctc gcggaggacg atccgctgcc cgacggcacc
      481 actcgcgtct atgtgtgcct cgacgacgaa gacgccgcgg tggccacctc tctcctgctg
      541 gctcgcgtca ccaggcgcgg cgtggtcacg tacctgcatc gagccgcacc gttcgagcac
      601 acccctgggt ggacgcactg gacgtattcg gcatcttcga caccgcctgc cgcggcgaca
      661 cgccatccgc caaagcctcg ctcatgccgt ggcgggacct gcccacgcac ctgcggcagt
      721 ccaacaacgc acaggtcggt cgcacggcga acctgtggtc cgagagccgc aagaaggaaa
      781 tcgacaccgt cgagcacctt ccggcgctcc tcgcgggagc cgacctgtac ctccgccgcc
      841 tgtctgcaac agaacactgg ccgacgaggc agatgcggca acaatgttaa tattagacta
      901 gcgtagatgt ggagttcggc gtcgtatcct cttgcagcat gacgaccacg atcacagtcc
      961 tcgagggcga ccagaccggt caggaactgc tcgaatgcgc gctccgggtc cttgaccccg
     1021 ccacatgcgg agtcgagctc aacctgcagc gattcgacct ctcgctgtca aatcgccgcg
     1081 ctacgaacaa taccgtggtg tttgctgctg cagacgccat gaaggagtgc ggattggggc
     1141 tgaaggcagc gacgattaca cctgaggtag ccggtgacgt ggggagcccc aacgccatcc
     1201 tccgcgagcg ggtgaacggc aaggtgatcg tgcgaaccgg ccgaaggatt cccggggttg
     1261 cctcgatcgc cggagtcagc gcgcccatca gcgtgatccg aatggccgtc gatgatgcct
     1321 acggcgccaa agagtggcgt gagggagctg gcgacgatga ggttgcatac cgcacggaga
     1381 agatcacccg gtcgacgtgc agggcggttg cggagttcgc ctttgtacaa gcaagacgga
     1441 cccgtgcgcg ggtatttggt ggacccaagt acacggtgtc gccagtctac gaggggatgc
     1501 tgaaggagga gatggacacc gcagccgggc gccacagcga tgtgccgtac gaaccacaac
     1561 tgatcgacgc gacatacgca ctcctactca cacagggtgg ggatccgctg gtaattcctg
     1621 cactcaatcg cgacggcgat tgtctgagcg atttcgtgct gcagctgttt ggcagcattg
     1681 caggggccga gtcgattctg atcggcttcc aaggcaacag caccattccg gaagttgtga
     1741 tggcggaggc gccgcacggc actgcccccg ctttagaggg caagaacatc gccaacccaa
     1801 tggcgatgat gttggcgtgc gcgtcgctgt tgggatttgt ccgggaaccg tccgcgaagc
     1861 gcgcctcccg cgccatatac gaagcgatct tcgagacggt gcgcagcggc accaagaccg
     1921 ccgacctggg cggtcactgt cttaccgacg agttcaccga cacggtaatc gacggcgtcc
     1981 gggcaaagct ggaaatttgg gagtcgatcg gcgtcgattg accgtgctga tggcacgcgt
     2041 gtcccttgac ccc
//
LOCUS       DASYLX010000785         6621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1359744,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000785 DASYLX010000000
VERSION     DASYLX010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359744"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGP27_18350"
     CDS             complement(<1..440)
                     /locus_tag="VGP27_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7853293.1"
                     /translation="MKFYVSVAFRETKEAIEIARAADDLGYAGIGIPDHVVNLETLAT
                     PYPYTRDGERRWEPFTEWPDPWVLVGAIAQVTTRLKFVTTVYIPAMRDPYAAAKAIGT
                     AAYISDGRVELGIGVGWCEEEFALMGQKFERRGKRTDEMLELFRA"
     gene            complement(437..1060)
                     /locus_tag="VGP27_18355"
     CDS             complement(437..1060)
                     /locus_tag="VGP27_18355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7853294.1"
                     /translation="MEFSFEEISVAEHERLQVLYSPLTDSIRRLVQAAIQSGADEATI
                     REAQSLVDAAVEKLGGVGRPDQVPLAVGNRPVVSGNPVTGFRNAIAPPLVVHEDPDGL
                     WWSEFELGEQYQGPPGWVHGGVLAMVLDQLLGEAASDGLTKPLFTGTITLRYLRGTPL
                     GRLRAEAAIERTDGYKTFASGHMSDAAGKTVEAEGIFIKPAWARDAG"
     gene            complement(1065..4019)
                     /locus_tag="VGP27_18360"
     CDS             complement(1065..4019)
                     /locus_tag="VGP27_18360"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEV7853295.1"
                     /translation="MAKPATQRPKLPSVGRLGLVEPSASADLEQLGWNTDAHVELLWS
                     LSRAPDADVALKTMVRLAEAAGRDWDELNQAVLKDKALRGRLFGVLGSSLALGDHLVA
                     NPRSWHLLAGNVALPSREELRVELAAAAESASGTGDELSVLRTLYRDRLLVLAGFDLA
                     SLVENEPVLPFTEVGEHLSDLADAALAAALKVATRSVCGVDGEAPRLAVIAMGKCGAR
                     ELNYVSDVDVIFVAEEADAVTTRVAGEMMRFASEAFFEVDAGLRPEGKHGQLVRTLES
                     HVAYYQRWAKTWEFQALMKARPAAGDAELGREYIAALTPMVWAACEREDFVPEVQAMR
                     RRVESLVPADERARELKLGTGGLRDVEFAVQLLQLVHGRNDESLHVASTIDALAALSV
                     GGYIGRDDAANLTASYEFLRLLEHRLQLQRLKRTHMLPDEDDDEAMRWLARAAHMRPD
                     GQHDALGVLREELKRQSHRVSRLHAKLFYQPLLEGVGQPAMLTPDAAERQLAALGYEG
                     PHSALTHLAALTGQSGRRGRVQQVLLPTLLDWLSDTPDPDAGLLSYRRISDELSDHRW
                     YLSTLRDEGAVAKRMMQVLGTSAYVPDLLMRAPEVIRQYADGPGGPKLMEVEPDGVAR
                     ALVASAGRHADPVRAIAAARTLRRRELARVASADLLGMLEVVDVCKALTSVWVAVLQA
                     ALEAVIRGSTPPNGEAPARIAVIGMGRLGGAELGYGSDADVMFVCEPVPGVDESAAVR
                     WSITVAEQVRSLLGTPSVDPPLEVDAGLRPEGRNGSLVRTLSSYAMYYEKWAQPWEIQ
                     ALLRAHRVAGDEDLGERFLLMADKTRYPAGGVSAEAVREIRRIKARVDAERLPRGADP
                     NTHTKLGRGGLADVEWTVQLLQLRHAHKIPALHNTSTLQTLDTIGAAELIAAGDVELL
                     REAWLTVTRARNALVLVRGKPTDQLPGPGRQLNAVAVAAGWDTDDGGEFLDNYLRVTR
                     RAKTVVRKVFGD"
     gene            complement(4065..5405)
                     /gene="glnA"
                     /locus_tag="VGP27_18365"
     CDS             complement(4065..5405)
                     /gene="glnA"
                     /locus_tag="VGP27_18365"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV7853296.1"
                     /translation="MDRQKEFVLRTLEERDIRFVRLWFTDVLGYLKSVAIAPAELEGA
                     FEEGIGFDGSSIEGFARVSESDTVARPDPSTFQVLPWTSSAGQHHSARMFCDITMPDG
                     SPSWADSRHVLRRQLAKASDLGFSCYVHPEIEFFLLQPGPDDGSSPVPADNGGYFDQA
                     VHDSAPNFRRHAIDALEQMGISVEFSHHEGAPGQQEIDLRYADALSMADNVMTFRYVV
                     KEIALGEGVKASFMPKPFAEFPGSAMHTHMSLFEGENNAFYSPDDPLQLSDTGKSFIA
                     GILEHATEISAVTNQWVNSYKRLVHGGEAPTAASWGFANRSALVRVPMYTPRKASSRR
                     IEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWTLTPEERRAMGYKELP
                     GSLGVALSEMENSELVAEALGEHVFDFFLRNKHTEWENYRSHVTPFELRTYLSL"
     gene            complement(5469..>6621)
                     /locus_tag="VGP27_18370"
     CDS             complement(5469..>6621)
                     /locus_tag="VGP27_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853297.1"
                     /translation="RRFDLVGFDPRGVGHSTPELRCRTDAEFDAYRREPNADYSSAGV
                     AHIEDLYHQFTQSCADRVGKDFLANVGTATTARDMEVVRAALGENQISYLGFSYGTEI
                     GAAYAEAHPDRIRAMVLDGAVDPSLDPLEENLRQMAAFQQAFEDYAADCAQLAGCPLG
                     NDPAQAVNRYHQLIDPLVTQPAATSDPRGLSYQDAITGTVNALYSRQYWKFLTSGLLG
                     LQRGSDPGDLLLLADDYLNRLPSGHYQNSQDAFYAIRCVNSQFPTDPAVWAAADQRAR
                     QAAPFLSYGSFTGLAPRDICSTWPVPATAGPHTAVSPGPGKVVVVSTTHDPATPYQAG
                     VDLARQMDAGLITFDGTQHTVVFNGDACVDTAVVAFLIDGVQPPEGLRC"
BASE COUNT         1137 a         2412 c         1982 g         1090 t
ORIGIN      
        1 gcccgaaaca gctcgagcat ctcgtccgtt cgcttgcccc gccgctcgaa cttctggccc
       61 atcaacgcga attcttcctc acaccagccc acgccgatgc ccagctcgac ccgcccgtcc
      121 gagatatatg ccgccgtgcc aatcgctttc gccgccgcgt acgggtcccg catcgccggg
      181 atgtacaccg tcgtcacgaa cttcagccgc gtcgtcacct gcgcgatcgc cccgaccaac
      241 acccacggat ccggccattc cgtaaacggt tcccaccgcc gctcgccatc acgcgtatag
      301 gggtacggcg tcgcaagcgt ctccaaattc acaacatgat cgggtatccc gataccggca
      361 tagcccagat catcagcagc acgcgcgatt tcgatagctt ccttggtttc ccggaacgca
      421 acgctgacgt agaacttcat cccgcgtctc gcgcccacgc cggcttgatg aagatgccct
      481 ccgcctccac cgtcttaccc gctgcgtcgc tcatgtgacc gctcgcgaat gtcttgtatc
      541 cgtccgtccg ctcgatcgcc gcttccgccc tcaaccgacc cagcggcgtg cccctcagat
      601 accgcagcgt gatcgttccc gtgaacagcg gcttggtcag cccgtcgctc gccgcctcac
      661 ccagcagctg gtccaacacc atcgcaagca ccccgccgtg cacccatccc ggcggaccct
      721 ggtactgctc ccccaactcg aattcgctcc accacaagcc atccggatct tcgtgcacga
      781 cgagcggggg cgcgatcgca ttgcggaacc ccgtcaccgg gttgcccgac accaccgggc
      841 ggttacccac agcaagcggg acctggtccg gccgcccaac gccacccagc ttctcgacgg
      901 ccgcgtcgac caatgactgt gcctcgcgaa tcgtcgcctc gtcagctccg ctctgaatgg
      961 ccgcctgcac cagccgacga atcgaatcag tcagcggcga gtacagcacc tgcaatctct
     1021 cgtgctcggc aacactaatc tcctcgaagg agaactctat gccctcagtc accgaacacc
     1081 tttcgtacca ccgttttcgc acgacgggtc acccgcagat agttgtcgag gaattcgccg
     1141 ccgtcgtcgg tgtcccagcc cgcggccacc gcgaccgcgt tgagctgccg gcccggcccg
     1201 ggcaattggt cagttggctt gccgcgcacc aacaccaacg cgttgcgggc ccgcgtcacg
     1261 gtcagccacg cctcgcgcaa cagctcgacg tcacccgctg caatcaactc cgccgcgccg
     1321 atcgtgtcca gcgtctgcag cgtcgacgtg ttgtgcaacg ccggaatctt gtgcgcatgt
     1381 cgcagctgca gcagctgcac cgtccactcg acatcggcca gcccgccacg accaagcttg
     1441 gtgtgcgtgt tgggatctgc cccccgtggc agccgctctg catcgacccg ggctttgatc
     1501 cttcggatct cccgcaccgc ctcggccgat acgccacccg ccgggtaccg cgtcttgtcg
     1561 gccatcagca ggaaccgctc gccgagatcc tcgtcgcccg caacccgatg cgcccgcaac
     1621 agcgcctgaa tctcccacgg ctgcgcccac ttctcgtaat acatcgcata tgacgacaac
     1681 gtccgcacca gcgagccgtt gcgcccctcg ggccgcagac cggcgtcgac ctcaagcggc
     1741 ggatccacac tgggcgtccc caacaacgaa cgcacctgct cggcgaccgt gatcgaccaa
     1801 cgcaccgcag cggattcgtc tacgccaggc acgggttcgc agacgaacat cacgtcggca
     1861 tcggaaccgt agcccagctc ggcgccaccg agccggccca taccgatcac cgcaatccgc
     1921 gcaggcgctt caccattcgg cggcgtggag ccgcgaatca ccgcttccaa cgccgcctgc
     1981 agcaccgcaa cccacaccga cgtcaacgcc ttgcagacat caaccacttc gagcatgcct
     2041 agtaggtccg ccgacgcgac acgcgccagt tcccgacgcc gcaacgtccg cgccgccgcg
     2101 atcgcacgca ccggatccgc atgtcgcccc gccgatgcga ccagagcccg cgccaccccg
     2161 tccggctcga cctccatcag cttcgggcca ccaggtccgt cagcgtactg ccgaatcacc
     2221 tccggcgccc gcatcaacag atccggcaca tatgccgatg tccccaagac ctgcatcatc
     2281 cgcttggcca ccgcgccctc gtcgcgcagc gtcgacagat accaccgatg gtcggacagc
     2341 tcgtcgctga tccggcgata cgacagcagg ccggcatccg ggtcgggcgt atccgacagc
     2401 caatccaaca gcgtcggcag cagcacctgc tggacccgac cacgtcgtcc gctctgtccg
     2461 gtcagcgccg cgagatgagt cagagcactg tgcggtccct cgtatcccaa tgcggcgagc
     2521 tgacgttcgg cggcatcggg cgtcagcatc gccggctggc cgaccccctc cagcaacggc
     2581 tggtagaaga gtttggcatg caggcgcgac acccggtggc tctgccgctt gagctcttcc
     2641 cgcaacaccc cgagcgcgtc gtgctgaccg tcgggtcgca tgtgagccgc gcgtgccagc
     2701 caccgcatcg cctcgtcgtc gtcctcgtcg ggcagcatgt gcgtgcgctt gagccgttgc
     2761 agctgcagcc tatgctcgag aagccgcaga aactcatacg acgcggtcag gttcgccgca
     2821 tcgtcgcggc cgatgtaccc gcccacactc agggccgcca gcgcgtcgat cgtcgatgcc
     2881 acatgcagcg actcgtcgtt gcgtccgtgc accaactgca gcagctgcac cgcgaactcc
     2941 acatcgcgca gcccgccggt gccgagcttg agctcacgag cccgttcgtc ggcgggcacc
     3001 agcgactcga cgcggcgccg catcgcctgc acctcgggca cgaaatcctc gcgctcgcag
     3061 gccgcccaca ccatgggcgt cagcgccgcg atgtactccc tgcccaattc cgcgtcaccg
     3121 gccgcggggc gcgccttcat cagcgcctgg aactcccagg tcttggccca gcgctggtag
     3181 taggcgacgt gcgactcaag tgtccgcacc agctggccgt gcttgccttc cggccgcagc
     3241 cccgcgtcga cctcgaagaa cgcctccgac gcgaaccgca tcatctcgcc tgccacccgg
     3301 gtcgtgacgg cgtcggcctc ctcggcgacg aagatgacgt cgacgtcgct gacgtagttc
     3361 agctcacggg cgccgcactt gcccatcgcg atcaccgcca gccgcggcgc ctcgccgtcc
     3421 acaccgcaca ccgaccgcgt cgccaccttc agcgcggcgg ccagtgcggc gtcagccaga
     3481 tccgacaggt gctccccgac ttcggtgaac ggcagcaccg gctcgttctc gaccaacgat
     3541 gcgaggtcaa aaccggcgag caccagtagc cggtcgcggt acagcgtccg caacacagac
     3601 agctcatcgc ctgtgccact tgccgattca gcagctgcgg ccaactcgac gcgcagttct
     3661 tccctcgacg gcaacgccac gttgccggcc agcaggtgcc acgaccgcgg gtttgcaacc
     3721 aggtggtcgc ccaatgccaa tgatgagccc agcacaccga acagcctgcc gcgaagcgcc
     3781 ttgtctttga gcaccgcctg gttgagctcg tcccagtccc tgcctgccgc ctcggccagc
     3841 cgcaccattg tcttcagcgc gacgtcggca tcgggcgccc gggacagcga ccacagcagt
     3901 tcgacgtgcg cgtcggtgtt ccagccgagc tgttccagat cggcggaggc cgacggctcg
     3961 accagcccta gccggccaac gctgggcagc ttcggtcgct gcgtcgcggg tttggccaca
     4021 caaaacgtta tcgcagaccg tagcgcaggc ccgttcgcgg cccgctacag cgacagatag
     4081 gtcctcagct cgaacggtgt gacgtggcta cggtagttct cccactcggt gtgcttgttc
     4141 cgtaggaaga agtcaaagac gtgctccccc aacgcttcgg cgaccaattc cgagttctcc
     4201 atctccgaca gcgcgacgcc gaggctgccc ggcaactcct tgtagcccat cgcgcgccgt
     4261 tcctcgggag tgagggtcca gacgttgtcc tcggcctggg gaccgagcac gtagcccttc
     4321 tccacaccgc gcaggcctgc tgccagcagc acggcgaacg tcaggtacgg gttgcatgcc
     4381 gagtcgggac tgcgcacctc gatgcggcgc gacgacgcct tccgcggcgt gtacatcggc
     4441 accctgacca acgccgagcg gttggcgaag ccccacgacg ctgccgtcgg cgcctcgccg
     4501 ccgtgtacca gccgcttgta cgagtttacc cactggttcg tcaccgcact gatctccgtg
     4561 gcgtgctcca aaatgcctgc gatgaacgac tttccggtat cggacagctg cagcgggtca
     4621 tccgggctgt agaacgcgtt gttttcgccc tcgaacaggc tcatgtgggt gtgcatcgcc
     4681 gagccgggaa attccgcgaa cggcttgggc atgaacgacg ccttcacgcc ctcgcccagc
     4741 gcgatctcct tcacgacata ccggaacgtc atcacgttgt cggccatgga cagggcatcg
     4801 gcgtaccgca gatcgatctc ctgctgaccg ggcgcaccct cgtggtggct gaactccacc
     4861 gagatgccca tctgctccag cgcgtcgatg gcatgcctgc ggaagttcgg cgccgaatcg
     4921 tggacggcct ggtcgaagta accgccgttg tcggcaggaa ccggcgagga gccgtcgtcg
     4981 ggccccggct gcagcaggaa gaactcgatc tccgggtgga catagcagga gaagcccaag
     5041 tcactggcct tggccagctg cctacgcagc acgtgcctcg agtccgccca tgacggcgag
     5101 ccgtcgggca tggtgatatc gcagaacatt cgcgcggagt ggtgctggcc ggcgctcgag
     5161 gtccacggca gcacctggaa agtagatgga tccggcctgg cgacggtgtc ggattccgac
     5221 acacgggcga acccctcgat cgacgagccg tcgaagccga tgccctcctc gaaggccccc
     5281 tcgagctccg cgggtgcgat tgccaccgac ttgaggtaac cgagcacatc ggtgaaccac
     5341 aaccggacga atcggatgtc ccgttcctcc agcgtgcgca gcacgaattc cttctggcgg
     5401 tccataatcg cagcgtaggc accggctgtt aaatccgtgt tacggagccg atgcgacctg
     5461 acgccggctc aacagcggag cccttcgggg ggctggacac cgtcgatgag aaacgccaca
     5521 acggcggtgt cgacgcaagc gtcgccgttg aacaccacgg tgtgctgggt gccgtcgaag
     5581 gtgatcaggc cggcgtccat ctgccttgcg aggtcaacac cggcctgata cggcgtggcc
     5641 ggatcgtgcg tcgtggacac gacgacgacc ttgcccggac ccggcgacac cgcggtgtgc
     5701 gggcccgccg tcgcgggcac gggccacgtc gagcagatgt cgcggggagc aagacctgtg
     5761 aaggagccgt aactgaggaa tggggcggcc tgacgggctc gttggtcggc ggcggcccac
     5821 acggcggggt cggtcgggaa ctgcgagttc acgcagcgga tggcgtagaa ggcgtcctgc
     5881 gagttctggt agtggccgga cggaaggcgg ttgaggtagt cgtcggcgag cagcagcagg
     5941 tcgcccgggt cgcttccccg ctgcagcccg agcagcccac tcgtcaaaaa cttccagtac
     6001 tgccgggagt agagcgcgtt gacggttccg gtgatcgcgt cttgatagct caggccgcgg
     6061 ggatccgacg ttgcggcggg ctgcgtgaca agtgggtcga tcagttggtg gtaccggttg
     6121 acggcctgcg cggggtcgtt gccgagcgga catccggcca gctgcgcaca gtcggcggca
     6181 taatcctcga atgcctgttg aaaagccgcc atctggcgca ggttctcctc gagagggtcc
     6241 aggctcggat cgacggcgcc gtcgaggacc atggcccgaa tccggtccgg gtgtgcctcg
     6301 gcgtatgccg ctccgatctc cgtgccgtac gagaacccga gatagctgat ctggttctcg
     6361 ccgagcgccg ctcgcaccac ctccatgtcc cgcgccgtcg tcgcggtgcc gacgttggcc
     6421 aggaaatcct tgcccacccg gtcggcgcag ctctgcgtga actggtggta cagatcttcg
     6481 atgtgcgcga cgcccgccga gctgtagtcg gcgttcggtt cccttcggta agcgtcgaat
     6541 tcggcgtcgg tgcggcaacg cagctcaggc gttgaatgcc cgacgccgcg gggatcgaag
     6601 ccgacgaggt cgaaccggcg a
//
LOCUS       DASYLX010000786         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2348389,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000786 DASYLX010000000
VERSION     DASYLX010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348389"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VGP27_18375"
     CDS             complement(<1..627)
                     /locus_tag="VGP27_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853298.1"
                     /translation="MLHRTARLAIAAPRAVIALAALFTVALGVFGLPVANKLSASGFQ
                     DPTSESSRASDLLTGKFHQGDVQLLIVVSSPDGVDSQNATQAGLDIVDHLRRSPHVAD
                     ISSPWTSPPSAAHGLISKDGTAGLIVAGIVGDEADQQKYAKELSDQVAHDRGGATIRS
                     GGVAMVNIQITNQSQRDLLLMESFAIPLSFIVLVWVFGGLLAAALPVAV"
     gene            767..1513
                     /locus_tag="VGP27_18380"
     CDS             767..1513
                     /locus_tag="VGP27_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEV7853299.1"
                     /translation="MAGHVEVRVYAELNDFLPRESRGLTLRRPVRPHQTVKDVLEATG
                     IPHTEVDLIVVNGQAQGFTHRPAPGDRIAAYPMFEALDIGSTARLRPVPLRDTRFVVD
                     VNLGRLAQLLRLVGFDAWWTSNADDETLVDISLAQRRILLTRDRGLLKRRIVTHGVFV
                     HSDDPQAQTLEVIRRLDLGERLAPLTRCMRCNGELVAVRKEEVIDRLEPMTRRYYSVF
                     VRCADCDRIYWPGSHHAKLLRLVEQVRQQL"
     gene            complement(1623..>1860)
                     /locus_tag="VGP27_18385"
     CDS             complement(1623..>1860)
                     /locus_tag="VGP27_18385"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEV7853300.1"
                     /translation="ARFEFRWHDQFALSLDPDTARQFHDETLPAEPAKTAHFCSMCGP
                     KFCSMRISHEVRDAMAEKSREFAEHGNRVYLPLD"
BASE COUNT          323 a          566 c          656 g          315 t
ORIGIN      
        1 gacagcgacc ggaagggcag ccgcgagcag gccgccgaac acccagacca gcacgatgaa
       61 gctcagcggg atggcgaacg attccatcag cagcaggtcg cgctgcgact ggttggtgat
      121 ctggatgttg accatcgcga caccgcccga gcggattgtc gcaccgccgc ggtcgtgggc
      181 cacctggtcg gacagctcct tggcgtactt ctgctggtcg gcctcgtcgc cgacgatgcc
      241 ggcgacaatc agtccggcgg tgccatcctt gctgatcaaa ccgtgggcgg ccgatggcgg
      301 tgaggtccac ggtgagctga tatccgcgac gtgaggcgac cggcggagat ggtcgacgat
      361 gtcgagaccc gcctgcgtgg cgttctggct gtcgaccccg tcggggctgg acaccacgat
      421 gagcaactgc acgtcgccct ggtgaaactt gccggtcaac agatcggatg cgcgggacga
      481 ctcggacgtc gggtcctgga atccgctggc ggatagcttg ttggccacgg ggagcccgaa
      541 gacaccgagc gcgacggtga acagcgccgc gagtgcgatg acggcgcgcg gcgcggcgat
      601 agccagtcgg gcggttcgat gtagcagggt cgatcctccg ctctgtggac tggccaccac
      661 gaggcggaga cttccgcggt ctccaaaaga gtcacgaagt gaggaggcga acggttcaga
      721 tcgtccgctg attgcggccg gctgaggctg cgcgagaaga tgaggaatgg cgggtcacgt
      781 agaggtgcgg gtgtacgccg agctcaacga cttcttgcca cgcgagtcgc gcggcctcac
      841 cctgcgccgc cccgtccgac cgcatcagac ggtcaaagac gtgctggagg cgacgggcat
      901 tccgcacacc gaagtcgacc tgatcgtggt gaacgggcag gcgcagggtt tcacacaccg
      961 cccagcgcct ggcgaccgca tcgccgcgta cccgatgttc gaagcgctcg acatcggctc
     1021 gacggcccgg cttcgtccgg tgccgctgcg cgatacgcgt ttcgttgtcg acgtcaacct
     1081 tggtcgactg gcgcagctct tgcggctggt cgggtttgac gcgtggtgga cgagtaatgc
     1141 cgacgacgag accttggttg atatcagcct cgctcagcgg cgcatcctgc tgacgcgaga
     1201 ccgcggcctg ttgaaacgcc gcatcgtcac ccatggcgtg ttcgtccact ccgacgaccc
     1261 gcaagcccag acgctggagg tgatacggcg gctggacctc ggtgagcggc tcgcaccgtt
     1321 gacccgctgc atgcgatgca acggcgagct cgtcgcggtc aggaaggaag aggtcatcga
     1381 ccgcctcgag ccgatgaccc gccgctacta cagcgttttc gttcgttgcg cggactgcga
     1441 ccggatctac tggccagggt cgcatcacgc aaagctgctg cgtctcgttg aacaggtccg
     1501 ccagcagctg tgagcgtcgg atacgtcagc tacagcagag gttgtcgagt acttggtagg
     1561 cggcgttgca gccggggatg aaggtgatgc aggggtcgcg aatggcgggc agcccaggga
     1621 tgtcagtcga gcggcagata gacgcggttg ccgtgttcgg cgaattcccg ggacttctcg
     1681 gccatggcgt cgcggacttc gtggctgatg cgcatcgagc agaacttggg cccgcacatc
     1741 gagcagaagt gggccgtctt ggccggctcg gcgggaagcg tttcgtcgtg gaactgccgc
     1801 gcggtgtccg ggtcgagtga cagcgcgaac tggtcgtgcc accggaactc gaagcgcgcc
//
LOCUS       DASYLX010000787         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3446002,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000787 DASYLX010000000
VERSION     DASYLX010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446002"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VGP27_18390"
     CDS             <1..919
                     /locus_tag="VGP27_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853301.1"
                     /translation="RVQKVSSEGDGLALQVRTTNADEFVVTAKQCVLATGIPILDRGG
                     FFARLKPQRSYCMAYRVPGDITSGMYLSTDSPTRSVRYAPTVDGQRLIVGGAGHPVGH
                     EKHPAASVQELDAWAKLHYPGAMQTHYWSAQDYSPIDELPYVGPILPGNDQIFVATGF
                     DKWGMTNGTAAALALAGRILGGRIDWAEAFASWSPHELSGIPKAMQMNLEVALYLARG
                     WITPVTRLGDRTPEDGGVVSGPPWQLEGRSVVDGVERRVSPICPHLGGIVNWNDADES
                     WECPLHGSRFAPDGTRLEGPATRDLTAAQ"
     gene            931..1392
                     /locus_tag="VGP27_18395"
     CDS             931..1392
                     /locus_tag="VGP27_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853302.1"
                     /translation="MGDTDEIKRVKYRYLRALDTKHWDDFADTLTEDIVGDYGSSLGE
                     EHHFTDRDTLVEFMRASMPANVLTEHRVTHPEIDVDGDEATATWYLQDRVIVPDFNFM
                     LYGAAFYHDRYRRTPDGWRISATGYQRTYEVNMSTDAITNFKVTPGGALNI"
     gene            complement(1396..2019)
                     /locus_tag="VGP27_18400"
     CDS             complement(1396..2019)
                     /locus_tag="VGP27_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7853303.1"
                     /translation="MALGAVAAPMTLADYTPWFAPKVGDATQVISVVGAGGSSAKIDV
                     FQRTAAGWQAVRTGIPAFVGENGMAPETHDGQAKTPMGIFTLDFAFGTAPNPGGGLQY
                     VEVGPDHWWDGDMKSPTYNTMQVCKKSQCPFRTDPASGTENLQIPQYVHAVVMGVNKE
                     RVPGNGGAFFLHATDGGPTAGCVALDDGTLVEIVKWLQPGALIAVTQ"
     gene            2123..>3122
                     /locus_tag="VGP27_18405"
     CDS             2123..>3122
                     /locus_tag="VGP27_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7853304.1"
                     /translation="MRFRRKPEGLRGLTEIGQGLGTLDREVFEAVAESPSPLLDTTMP
                     RLSRAADHSKLWFVIAAGLIATRHKATVRGAIRGVVSLAVTSAFTNQFAKRVWKRPRP
                     NRMLVPLARQSRRFPTSNSLPSGHSASAAAFAVGVGLENPPVGLGLALLAGLVGLSRV
                     AVGAHYPGDVFAGFGIGAGIAVLGARIVPPITPTKIPTADPLRVTTLPRPDGAGVVLV
                     INPASGSGTGARVVDEVREALPKAEIVELGDGDDIEEALRDAAKRTEVLAVGGGDGTV
                     SCAAGIAVERGIPLAVFPGGTFNHFAKDIGCDSVAKTVEAIRRGDVSCVDLVCLNDE"
BASE COUNT          531 a         1039 c         1037 g          515 t
ORIGIN      
        1 tcgggtgcaa aaggtttcaa gtgaaggcga cgggttggcg ctgcaggtgc ggacgacaaa
       61 cgccgacgag ttcgtcgtca cggcgaagca gtgtgtgctc gccaccggta ttcccattct
      121 ggatcgcggc gggttcttcg cgcggctcaa accgcagcgg tcctactgca tggcctaccg
      181 ggtgccgggt gacatcacca gcggcatgta cctctcgacg gactccccga cccggtcggt
      241 ccgctatgca ccgaccgtcg acggccagcg cctgatcgtc ggcggcgcgg gacacccggt
      301 gggccacgag aagcatccgg cggcgtcggt gcaggaactc gatgcctggg cgaagttgca
      361 ctatccgggc gcgatgcaga cgcattactg gtcggcgcag gactattcgc cgatcgacga
      421 actgccgtac gtcgggccga tccttcccgg caacgaccag atcttcgtcg cgacgggctt
      481 cgacaagtgg ggcatgacga acggaaccgc cgcggcgctc gcgttggccg gccggatcct
      541 cggcggccgc atcgactggg cggaggcatt cgccagttgg agtccccacg agctgtccgg
      601 aattccgaag gcgatgcaga tgaatctgga ggtggccctg tacctcgcaa ggggctggat
      661 caccccggtg acacggctcg gcgaccggac gcccgaggac ggtggagtcg tcagcgggcc
      721 gccgtggcag ctggagggcc gcagcgtcgt cgacggcgtt gagcgtcgag tctcaccgat
      781 ctgcccacat ctgggcggca tcgtcaattg gaacgacgcc gatgaatcgt gggagtgtcc
      841 gttgcacggc tcaaggttcg cgccggacgg tacgcggctc gaaggtcctg cgacccggga
      901 cctgactgcg gctcagtagt atccactcgc atgggcgata ccgacgagat aaagcgggtg
      961 aagtaccgct acctgcgggc gctggatacc aagcactggg acgatttcgc cgacaccctc
     1021 accgaggaca tcgtcggtga ctacggcagt tcgctgggcg aggaacacca tttcaccgac
     1081 cgtgacacgt tggtcgagtt catgcgcgcc tcgatgcccg ccaacgtgct gaccgagcac
     1141 cgcgtgacgc accccgagat cgacgtcgac ggcgacgagg ccaccgccac ctggtatctg
     1201 caggatcgcg tgatcgttcc cgacttcaac ttcatgctct acggcgcggc gttctaccac
     1261 gaccgatacc ggcgcacgcc cgacgggtgg cggatcagcg caaccggata ccagcgcacc
     1321 tacgaggtga acatgtccac cgacgcgatc accaacttca aggtgacacc cggtggggcg
     1381 ctgaacattt gaggtttact gggtaaccgc gatcaacgca cccggctgca accacttcac
     1441 gatctcgacc agggtgccgt catcgagggc gacgcaacct gcggtcggac cgccgtcggt
     1501 ggcgtgcagg aagaacgcac cgccgttacc gggcacgcgt tccttgttga cgcccatcac
     1561 cacggcatgc acgtactgcg ggatctgcag gttctcggtg ccgctggcgg ggtccgtgcg
     1621 aaaagggcac tgcgacttct tgcacacctg catggtgttg taggtcgggc tcttcatatc
     1681 gccgtcccac caatggtcgg gcccgacctc gacgtactgc aggccgccgc cagggttcgg
     1741 cgcggtgccg aacgcgaagt cgagggtgaa gatgcccatc ggggtcttcg cttggccgtc
     1801 atgggtttcc ggcgccatgc cgttctcgcc gacgaaggcg gggatgccgg tccttaccgc
     1861 ttgccagccg gcggccgtgc gttggaagac gtcgatcttc gccgacgaac caccggctcc
     1921 cacaacggat atgacctggg ttgcgtcacc taccttcggg gcgaaccacg gcgtgtagtc
     1981 ggcgagggtc atcggtgcag caaccgcccc cagcgccata gtgagcgtgg ctatcagcga
     2041 taccaaccat gggcgcacgc gcccatggtt gcacaggagc aggccccggt tgggcagctg
     2101 gcctgctggg taagttggcg tcatgcgatt ccgtcgaaaa ccagagggcc ttcgcggcct
     2161 taccgagatc ggccagggcc tcggcaccct cgatcgcgag gtcttcgaag cggtcgccga
     2221 atcgcccagc ccgctccttg acaccacgat gccgcggctc agccgggccg ccgaccactc
     2281 caagctctgg ttcgtcatcg cggccggcct gatcgcgaca cggcacaagg cgaccgtacg
     2341 tggcgccata cgcggagtcg tgagcttggc cgtcaccagc gctttcacca accagttcgc
     2401 caagcgcgtc tggaaacggc caaggcccaa ccgcatgttg gtgccactcg cacggcagag
     2461 ccggcgcttt ccgacgtcga actcacttcc gtccggacac tcggccagtg ctgccgcatt
     2521 cgccgtcggt gtcggtctcg agaacccgcc cgtcggactg ggcctcgccc tgctcgcagg
     2581 tctggtgggc ctgtcgcgcg tcgccgtcgg cgcgcactat ccgggcgacg tgtttgcagg
     2641 cttcggcatc ggggcgggca tcgcggtgct gggcgcaagg attgtcccgc cgatcacgcc
     2701 cacgaagatc cccaccgccg atcctctacg ggtcaccacg ctgccgcgac ccgacggcgc
     2761 gggcgtcgtg ctggtgatca atccggcgtc gggcagcggc acgggggcgc gcgtcgtcga
     2821 cgaagtgcgc gaagcgctgc ccaaagccga gatcgtcgag ctcggcgacg gcgatgacat
     2881 cgaggaggca ttgcgcgacg cggccaagcg caccgaggtc ctcgccgtgg gtggcggcga
     2941 cggcaccgtg tcgtgcgcgg caggcatcgc cgtcgagcgc ggaattccac ttgcggtctt
     3001 ccccggcggc accttcaacc acttcgccaa ggacatcggc tgcgacagcg tggccaagac
     3061 ggtcgaggcg atccgacgcg gcgacgtgtc ctgcgttgac ctcgtgtgcc tcaacgacga
     3121 ac
//
LOCUS       DASYLX010000788         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1799798,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000788 DASYLX010000000
VERSION     DASYLX010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799798"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGP27_18410"
     CDS             <1..724
                     /locus_tag="VGP27_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217813.1"
                     /note="similar to the DNA and RNA helicase superfamily II
                     and eukaryotic DEAD family of helicases; longest known
                     protein in E. coli; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEV7853305.1"
                     /translation="LSRYSVAHAQTRASDPTVAGRWSALPAAELDSTVRAHFQAELLL
                     NRQGVLTKGAVAAEGLPGGFAMLYKVLTAFEDAGRCQRGYFVESLGGAQFAVASTVDR
                     LRSYLDSVDPERREYQTVVLAAADPANPYGAALPWPESAHRPGRKAGALVALVDGQLT
                     WFLERGGRTLLTFTDDAEAHHAAAGAVADLVGRGRVQSLLVEKVNGEPVLAPDAAGDQ
                     ASVVAALRDAGFARTPRGLRLR"
     gene            748..>1634
                     /locus_tag="VGP27_18415"
     CDS             748..>1634
                     /locus_tag="VGP27_18415"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7853306.1"
                     /translation="MGFRTALTEMFGIAHPVVLAPMGDVAGGVLAAAVSEGGGLGVVG
                     AGRGDPGWLTRECELARAGTRKPWGIGFLSWAIDSAVVDAAISQSPAAIMLSFGDPAP
                     FIDAIKAAGIPVIVQVTDLSEARRALDVGADVVVAQGSEAGGHGGGRATLPFVPAVVD
                     IAGPVPVLAAGGIADGRGLAAALVLGAAGALIGTRFEATHEALITAEAAKAITAASAE
                     DTTAGRALDVVRDSQWPARYPARTLRNRVTETWQGREAEIDDATKAAFRSGVDRGDLD
                     YLPIWAGEAVDMITGQDSAA"
BASE COUNT          231 a          560 c          599 g          244 t
ORIGIN      
        1 gctgagccgc tacagcgtcg cccacgcaca gacacgcgca tccgatccca ccgttgcggg
       61 acgctggtcc gcgcttccgg ccgccgagct ggattcgact gtgcgagccc acttccaggc
      121 cgagttgctg ctcaaccggc agggcgtgct gacaaagggg gccgttgcag ccgaaggcct
      181 ccccggtgga ttcgccatgc tctacaaggt gctcaccgcc ttcgaggacg cgggccgctg
      241 ccaacgcggc tacttcgtcg agtcgctggg cggcgcgcag ttcgcggtcg cgtcgacagt
      301 cgatcgactg cggtcgtatc tcgacagcgt cgatccggaa cggcgggagt accagaccgt
      361 ggtgctggcc gccgccgatc ccgccaaccc gtacggcgcg gcgctgccgt ggcccgaaag
      421 cgcccatcgg ccgggccgaa aggccggggc gctggtggcg ctcgtcgacg gacaactcac
      481 gtggttcctc gagcgcggcg gacgcacgct gctcacgttc accgacgacg ccgaagcgca
      541 tcatgccgcg gccggggcag tcgccgacct ggtcggccgg ggccgcgtgc aatccctgct
      601 ggtcgagaag gtcaacggcg agcccgtgct ggcaccggat gcggcgggcg accaggcctc
      661 cgtcgtggcc gcgctgaggg acgccggctt cgcccgcact ccacggggtc tccgactgcg
      721 ctgacagtcg gcttctagag tgctggcatg ggctttcgca cagcgttgac cgagatgttc
      781 ggcatcgccc atcccgtggt gctggccccc atgggcgacg tcgcaggcgg cgtgctggcg
      841 gcggccgttt ccgagggcgg cggccttggt gtggtcgggg ctggccgggg agacccggga
      901 tggctcacgc gtgagtgcga gttggcgcgg gccggcacgc gcaaaccatg gggtatcggt
      961 ttcctgtcct gggccatcga ttcggccgtc gtggacgcgg cgatttcgca gtcgcccgcg
     1021 gcgatcatgc tgtcgttcgg cgaccccgcg ccgttcatcg atgcgatcaa ggccgcaggc
     1081 attccggtga ttgtccaggt caccgactta tccgaggccc gtcgagcgct cgatgttggg
     1141 gcggacgtcg tcgtcgcgca gggctctgag gccggtggcc acggcggcgg acgcgccact
     1201 ctgcccttcg tgcccgcggt cgtcgacatc gcaggaccgg ttccggtgct cgccgcaggc
     1261 ggaatcgccg acggccgcgg actggctgcc gcgcttgtgc tcggcgctgc cggagccctc
     1321 atcggaacga ggttcgaagc cacgcacgaa gcgctgatca ccgcggaggc cgcgaaggcg
     1381 atcaccgccg ccagcgccga agacaccacg gcggggagag cgctcgacgt cgtccgcgac
     1441 tcgcagtggc cggcgcgata tccggcacgg acgctgcgca acagggtcac cgagacctgg
     1501 cagggacgcg aggccgagat cgacgacgcg acgaaggcgg cgttccgctc gggggtcgac
     1561 cgcggcgacc tcgactacct gccgatctgg gcgggtgagg cggtggacat gatcaccgga
     1621 caggacagcg ccgc
//
LOCUS       DASYLX010000789         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2676586,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000789 DASYLX010000000
VERSION     DASYLX010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676586"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGP27_18420"
     CDS             complement(<1..480)
                     /locus_tag="VGP27_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853307.1"
                     /translation="MAGEELDELYGVRPEEFTARRSELAAAANKRGDAAAAKRISAAR
                     KPTTAAWVVNQLVHANEDVKRSLTDLGERLRAAHAAMDGAAIRELSSEQRSLVDELAR
                     AAFEGVDVANPSAALREDVTGTLQAAVADPDVAGRLGHLAKAEQWSGFGDFGSATAVF
                     "
     gene            550..1467
                     /locus_tag="VGP27_18425"
     CDS             550..1467
                     /locus_tag="VGP27_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853308.1"
                     /translation="MVVAIDRPKLEGNVAVGEDRQIGFAEFGAPQGRAIFWLHGTPGA
                     RRQIPTEARAFAEEADIRLIGIDRPGIGSSTPHQYENVFEFTDDLRTIADTLGIDKMA
                     VVGLSGGGPYTLACAAAMPERVVAAGVVGGVAPPTGPDAIGGGVMGNLGTRVAPLLQV
                     AKVPIGVVATTLIRFIRPVAGPMADLYGLVSPPADRALLARPEIKAMFLDDLLNGSRR
                     QMAAPFYDIVAFARDWGFRLDEVKVHVRWWHGDADHIVPFAHGQHVVSRLPDAELLPM
                     PGESHLGGLGRAEEILRSMLELWDSAGAD"
     gene            1471..>1844
                     /locus_tag="VGP27_18430"
     CDS             1471..>1844
                     /locus_tag="VGP27_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853309.1"
                     /translation="MRAIVLLAQSAALDQAGTLHALGLGWTASSTPVPQHALVIFVEV
                     EWAELNKSFPIRAELVDSDGVRVAHTEHGVIKAKHKPVHPEGTPVTIPFTATIPPLKL
                     TAGQRYQWRVSIDDEMHEDWLAG"
BASE COUNT          305 a          635 c          612 g          292 t
ORIGIN      
        1 gaagaccgct gtcgctgaac cgaaatcacc gaagccggac cattgttcgg ccttggcaag
       61 gtgaccgagc cggccggcca catcggggtc cgcgacggcc gcctgcagcg ttccggtgac
      121 atcctcacga agcgctgccg atggattcgc cacatcgacc ccttcgaagg cggcgcgggc
      181 gagttcgtcg accagtgacc gttgctcgga ggacagctcg cggatggctg caccatccat
      241 cgcggcatgc gccgcgcgaa gccgctcccc caggtcggtg agcgaacgct tgacgtcttc
      301 attggcatgc acgagctggt tgacaaccca tgccgcggtg gtcggcttgc gggccgcgga
      361 gatccgcttc gccgcagcag cgtcaccccg cttattggcc gcggcggcga gctcgctgcg
      421 acgggccgtg aactcctcgg gcctgacgcc ataaagttcg tcgagctcct caccggccac
      481 aacgcaatga tgcgccacga tgtcgggaag ccggacaggg actcgagagc ggcatgcgaa
      541 actttcggca tggttgtcgc aatcgatcgc ccgaagctcg aaggaaacgt cgccgtcggc
      601 gaagaccgcc agatcggctt cgccgagttc ggcgcaccgc agggccgggc catcttctgg
      661 ttgcacggta cccccggcgc gcgcaggcag atccccaccg aggcgcgggc cttcgccgaa
      721 gaggcggaca tccgcctcat cggtatcgac cggccgggca tcggttcgtc gacgccccac
      781 caatatgaga acgtcttcga gttcaccgac gacctgcgga ccatcgcgga cacgctggga
      841 atcgacaaga tggccgtcgt cggcctttcc ggtggtggcc cctacacgct ggcatgtgcc
      901 gcggccatgc ccgagcgtgt ggtggcggcc ggcgttgtcg gaggggtcgc ccctccgacc
      961 ggaccagacg ccatcggggg cggcgtgatg ggcaacctcg gaacgcgcgt cgcgccactc
     1021 ctgcaggtgg cgaaggtgcc gatcggcgtg gtggccacca cgctgatccg gttcatccgc
     1081 ccggtggcgg ggcccatggc ggatctgtac ggactggtgt cgccgccggc cgaccgtgcg
     1141 cttctggccc gaccggagat caaggccatg ttcctcgacg atctgctcaa cggcagccgt
     1201 cggcagatgg cggcgccgtt ctacgacatc gtcgcctttg cacgcgactg gggattccgg
     1261 ctggacgagg tcaaggtgca cgtgcgttgg tggcacggcg acgccgacca catcgttccc
     1321 ttcgcgcacg gacagcatgt ggtgtcaagg ctgcccgacg ccgagctgtt gcccatgccg
     1381 ggtgagagcc atctcggcgg gctcgggcgc gccgaagaga tcctgcgctc gatgctcgag
     1441 ttgtgggaca gcgccggcgc cgattgacac atgagagcca tcgtgctgct cgcccagagc
     1501 gccgccctgg accaggcggg cacccttcac gcgctcggac tcggctggac ggcctcctcg
     1561 acacccgttc cgcaacatgc gctggtgatc ttcgtcgaag tcgaatgggc cgagctcaac
     1621 aagtcgttcc cgatccgcgc cgagctggtc gacagcgacg gtgtccgggt ggcgcacacc
     1681 gaacacggcg tcatcaaggc caagcacaaa cccgtacacc ccgagggcac cccggtcacg
     1741 attcccttca ccgccaccat ccccccgctc aagctcaccg ccggtcagcg ttaccagtgg
     1801 cgggtctcga tcgacgacga gatgcacgag gactggctgg ccgg
//
LOCUS       DASYLX010000790         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3005689,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000790 DASYLX010000000
VERSION     DASYLX010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005689"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VGP27_18435"
     CDS             complement(<1..1387)
                     /locus_tag="VGP27_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7853310.1"
                     /translation="MTTELVPEQVLPKVMTTFGLVSIYVFIICWITGSSVMAAGGWTA
                     IPMWVLGILTFLVPAGMAVAELGNLWPGEGGVYIWATRTMGETWGFIGGYLSWIPVIL
                     NAASSPAIVLQFLLLAFHAELGLTVSIILQLVILWGVVGLALAKLAANQRIMTTVFYI
                     YGVLTALIFVSGLVYALKNGSATPFTAHAALVPDFAGAGFLYGTVLLYLLGVETPYNM
                     GAEFLSVRKSGPKMIIWGSAALVVIYLLTTLGTMMALPSDQIDPVTGVIGMLGTAAPK
                     GVMEGAAIVLAVIVFVALMTYQVAYSRLIFVSGLERHLPRIFTHLNPRTRNPVTAVLI
                     QGVLSSLILVGLYSQSSMANVTIFLQGGLSTVWLISGFFFLFPVVIARKKYADRYASE
                     KFWRIPGGMVGVWITVIVGTLGTIGGVYYSFAKSWLTNVGVGDAEWMRWVGSIALGMF
                     VLGVVVYFFGRR"
     gene            complement(1481..2209)
                     /locus_tag="VGP27_18440"
     CDS             complement(1481..2209)
                     /locus_tag="VGP27_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853311.1"
                     /translation="MTAIEDRTDTTGLSLDHDRSPVIEISDVWKLHKVGDEVVKALVA
                     ADLTVMPGEFVCLMGPSGSGKSTLLNMIGGLDRPTKGTIKVAGRDTSTLTESQFAALR
                     HDTIGFIFQSYNLIPFLSAVENVELPLIFEPYDRKSLRKRALELLELVGLGHRVDHQP
                     TKMSGGEQQRTAIARSLISSPTLVLADEPTANLDHRTGEMVVRMLRDLCSTLGVTVVA
                     STHDPTVADEATRVVRMKDGQIAN"
     gene            complement(2211..2777)
                     /locus_tag="VGP27_18445"
     CDS             complement(2211..2777)
                     /locus_tag="VGP27_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV7853312.1"
                     /translation="MMRKGLSYGWLSARRRIAEMVLPVVTTATGAFLVVIVFGMSEGI
                     RGQSASLGHADEIGRAVVLIAVTVLLVGVAEVAVTTTRTVARRTRELGVLSANGVPRK
                     PVIAALLVEPVIAAVLGALAGAGLAVLTGVALGMSGVVATGVSAAGLMLGVAIAVGVS
                     IVAAFFTSIVPTWNAASRPPIRSLTTGG"
     gene            complement(2778..3875)
                     /locus_tag="VGP27_18450"
     CDS             complement(2778..3875)
                     /locus_tag="VGP27_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853313.1"
                     /translation="MCLVRFALANIRRRPERFALSVLGIALAIACVTVVRTISASFAI
                     TGADSVTDVLGGAALWAVPAAGVKYDADAQALVANGPAPTFAAPDGWTAARTLAGVTE
                     INGDTVSLRGNDETPAGQAILGIPVAQRLGLNSGDRVVVGGQSLTVDVSGGGQSITVA
                     SPLAQTVVGSNGWWLIFPPPGDEKRRDIGQTFGSAIGLESTSDPSVMPDPNGRGLIYD
                     TVGGSGPLTFEQKFSALFSGKVTGSTLGLISIIGLGLGFVIAVSSFLAAVAERRREFG
                     IMSSIGLADEVLYFFLVESAMVFVAAYVVGVLGAGAAVALVIPGIATPTAWLQAAGMV
                     AAFLPAMAIVGALVPVHRLLQQRPVALLGDR"
     gene            complement(4061..>4393)
                     /locus_tag="VGP27_18455"
     CDS             complement(4061..>4393)
                     /locus_tag="VGP27_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV7853314.1"
                     /translation="RLSASRRITERLSQTYDAVLMPTLASETPRIGHLDPTAPYEQVM
                     DRIIDWVGFTPLQNATGDPAISLPLAESASGLPVGMMLSTVRGGEATLLELAYELEQA
                     HPWPRIDG"
BASE COUNT          847 a         1446 c         1407 g          693 t
ORIGIN      
        1 accgtcggcc gaagaagtac acgaccactc cgagaacgaa catgcccaac gcgatgctgc
       61 cgacccatcg catccactcg gcgtccccta ctccgacgtt ggtcagccag gacttggcga
      121 acgagtagta gacgccgccg atggtgccca acgtgcccac gatgacggtg atccagaccc
      181 ccaccatgcc gccggggata cgccagaact tctcgctggc gtagcggtcg gcgtactttt
      241 tgcgcgcgat gacgaccggg aacaggaaga agaacccgga gatcagccag acggtggata
      301 ggccaccctg gaggaagatg gtgacgtttg ccatgctgct ctgcgagtac aaccccacca
      361 ggatcagcga cgagagcaca ccctggatga gcaccgcggt caccggattg cgcgtgcgcg
      421 ggttgaggtg ggtgaagatc cgcggcaggt gacgttcgag acccgacacg aagatcagcc
      481 gggagtaggc cacctgatag gtcatcagcg ccacgaacac gatcacggcc agcacgatcg
      541 ccgcaccttc catcacgccc ttcggcgcgg cagtgccgag catgccgatc acaccggtca
      601 ccgggtcgat ctggtccgac ggcagcgcca tcatggtgcc cagcgtggtc agcaggtaga
      661 tgaccaccag cgccgcggag ccccaaatga tcatcttcgg cccgctcttg cgcaccgaga
      721 ggaactccgc acccatgttg tacggcgtct cgacgcccaa caggtacagc agcacggtgc
      781 cgtaaaggaa gcctgcgccg gcgaagtcgg gtaccagtgc ggcgtgcgcg gtgaacggcg
      841 ttgccgaccc gttcttgagc gcgtagacaa ggcccgagac gaagatcagg gcagtcaata
      901 ccccgtagat atagaagacg gtggtcatga tgcgctggtt ggcagccagt ttcgccaacg
      961 ccagaccgac cacgccccag aggatcacca gctgcaagat gatgctgacc gtcagcccga
     1021 gttccgcgtg gaaggccagc agcaggaact gcagcacgat cgccggagac gacgcggcgt
     1081 tgaggatcac cgggatccac gacaggtaac cgccaatgaa gccccaggtc tcgcccatcg
     1141 tgcgggtggc ccagatgtac acgccgcctt cacccggcca cagattgccc agttcggcga
     1201 ccgccatgcc tgcgggcaca aggaacgtca ggatgccgag cacccacatg gggatggcgg
     1261 tccagccgcc cgccgccatc accgacgagc cggtgatcca gcagatgatg aacacgtaga
     1321 tggagacgag gccgaacgtg gtcatcacct tgggcagcac ctgctcaggt acgagctcgg
     1381 tggtcatcag cgcgtcgcgc ttagacggcg tcgcttcgcc ccgattgccc ctcgcgtcag
     1441 cggtgagttc ttgcgtcatt tctctcccaa agccgttcgg tcagtttgcg atttgtccgt
     1501 ctttcattcg gacgacacgg gtcgcttcat cggccaccgt ggggtcatgg gtgctggcga
     1561 cgaccgtgac gccgagcgtc gagcacaggt cgcgcagcat ccgcaccacc atctcgcccg
     1621 tacggtggtc cagattggcc gtcggttcgt cggcaaggac gagcgtcggg ctgctgatca
     1681 gggaacgcgc gatcgcggtg cgttgttgtt cgccacccga catcttggtc ggctggtggt
     1741 cgacccgatg gccgagcccg acgagctcga gaagctccaa cgcccgcttg cgcagagact
     1801 tgcggtcgta gggctcgaat atcagcggca gctcgacgtt ttccaccgcg gacaggaacg
     1861 gaatcaggtt atagctctga aagatgaagc cgatggtgtc gtggcgcagc gcggcgaact
     1921 gactctcggt cagcgtcgag gtatcccggc ctgccacctt gatggtgccc ttggtgggcc
     1981 ggtcgaggcc gccgatcatg ttcagcagcg tcgacttgcc gctgccgctt ggtcccatca
     2041 ggcagacgaa ttcgcctggc attacggtca agtcggcagc gacgagcgcc ttgaccacct
     2101 cgtcaccgac cttgtgcagc ttccacacgt cggagatctc gatgacggga gaccggtcgt
     2161 ggtcgaggga aagacccgtc gtgtcggttc tgtcttcgat ggcggtcatg tcaacctccc
     2221 gttgtcagag aacggatcgg tggccgcgac gccgcgttcc acgtgggcac gatgctggtg
     2281 aagaaggccg cgacaatgct gacgccgacc gcgatcgcga cgccgagcat caggcccgcg
     2341 gcggatacgc ccgtcgccac cacacccgac atcccgagcg cgactcccgt tagcacagcg
     2401 agtccggcac ccgccagggc gcccagtacc gctgcgatca ccggctccac gagcagcgcc
     2461 gcgatgacgg gcttacgcgg cacaccgttg gcactgagca caccgagttc acgggtcctg
     2521 cgggcgacgg ttcgagtggt ggtgaccgca acctccgcca ccccgacgag aagtaccgtc
     2581 accgcgatca gcacgacggc acggccgatc tcgtcggcgt gccccagcga cgcggactgt
     2641 ccgcggatgc cctcggacat gccgaacacg atcaccacca ggaacgcgcc ggttgcggtg
     2701 gtgacgaccg gcagcaccat ctcggcgatc cggcgccgcg cggacagcca cccgtaggac
     2761 aaccctttac gcatcatcta ccgatctccc aggagtgcga cgggacgttg ttgcagcagc
     2821 cggtgcaccg gaacgagtgc gccgacgatc gccatggccg gcagaaacgc ggcgaccatg
     2881 ccagcggcct gcagccacgc agtgggcgtc gcgattcccg ggatcaccag cgccacagcg
     2941 gcacccgcac cgagaacgcc gaccacgtag gcggcgacga acaccatcgc cgactcgacg
     3001 aggaagaagt acagcacctc gtcggccagg ccgatgctgg acatgatgcc gaactcacgc
     3061 cgccgctcag ccactgcggc gaggaacgac gacaccgcga tgacgaaacc gagcccgagc
     3121 ccgatgatcg agatgaggcc gagcgtggaa ccggtcacct tgccggagaa cagcgcagag
     3181 aacttctgct cgaacgtcag cgggccggag ccgccgacgg tgtcgtagat caagccgcgc
     3241 ccattgggat ccggcatcac cgacggatcc gatgtcgact cgagaccgat tgcgctgccg
     3301 aaggtttggc cgatatcccg ccgcttctcg tcacccggcg gcgggaatat caaccaccag
     3361 ccgttgctgc cgacgaccgt ctgcgccagc ggtgaagcga ccgtgatcga ctgaccgccg
     3421 ccggacacgt cgacggtcag gctctgtccc cccaccacga cgcggtcacc cgaattgagg
     3481 ccgagtcgct gcgccaccgg gatacccagg atcgcttgcc cggcaggcgt ttcgtcgttg
     3541 ccacgcaacg aaaccgtgtc accgttgatc tcggtgacgc cggcgagagt gcgggcggcg
     3601 gtccaaccgt ccggcgcggc gaacgtagga gccgggccgt tcgcaaccaa tgcctgcgca
     3661 tcggcgtcgt acttcacgcc cgcggcggga accgcccata gcgcagctcc gccgaggacg
     3721 tctgtcaccg aatcggcgcc ggtgatggcg aaactcgccg aaatggttcg gactactgtc
     3781 acgcaggcga tcgcgagcgc gatgccgagc acggacagcg cgaaccgctc gggtcgccgg
     3841 cggatatttg ccaacgcgaa ccggacaagg cacatgaatg tgttgccgga agtggtggcg
     3901 atcgtcgatg accgaagtga cgcgggagca gaggtctcca cgaacaagga tgttcggctc
     3961 gaaaagtttc gccgacttcc atgcccaatg tcggcgttgt taactcaggc ggttccgggt
     4021 tacggcgttg tgtcgaggcc gcaacggatt tcgttgccgg tcatccgtcg atgcgcggcc
     4081 aggggtgtgc ttgttcgagc tcgtaggcca actccagcag cgtggcctcg cctcctcgca
     4141 cggtcgacag catcatcccc accggcagac ccgatgcgga ttccgccagc ggcagcgaga
     4201 tcgccgggtc gccggtggcg ttctgcagcg gggtgaagcc gacccagtcg atgatccggt
     4261 ccatcacttg ttcgtagggc gccgtcggat cgagatgtcc gattcgtggt gtctcgctgg
     4321 ccagtgtcgg catcaacacg gcgtcgtagg tctgtgacag tcgttcggtg atgcgcctcg
     4381 atgccgacaa acg
//
LOCUS       DASYLX010000791         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2897233,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000791 DASYLX010000000
VERSION     DASYLX010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897233"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VGP27_18460"
     CDS             complement(<1..148)
                     /locus_tag="VGP27_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7853315.1"
                     /translation="MALLGPQATGLAAAAEDSSCSDVEVVFARGTFEAPGVGATGQAF
                     VDALS"
     gene            578..1369
                     /locus_tag="VGP27_18465"
     CDS             578..1369
                     /locus_tag="VGP27_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853316.1"
                     /translation="MTERSDRARSRTFVVTGTASGIGLAVAERLLAEGGTVVGADVNP
                     PPTLGERFSFVTADLSDESAVAEVFAALPDRLDGVVHAAGIASGGPVHLLDKAEWDRV
                     IAINLTATFLVAKAALSRMIEQPRVDSERGSIVTLASVEGLEGTAGGSGYNAAKGGVV
                     LLTKNIALDYGPIGIRANAICPGFIATPMLDSVFRMPGMEHTLRSITDEHALQRLGRP
                     DEIAAMAAFLVSSDASFVTGQAIAVDGGYTAGRDHGVVRLFGLPD"
     gene            complement(1398..2000)
                     /locus_tag="VGP27_18470"
     CDS             complement(1398..2000)
                     /locus_tag="VGP27_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7853317.1"
                     /translation="MTGPRDQRSRRPTSDDDTVEVPLRSARSADAPPPVEPWWKNANQ
                     RIPSPPRTPSPPPVPRPAKPPVDRNGRAPAPARNRHARPTSARQKSGQLALLIAVGVV
                     AISVAAVLLMVVLSRFDVLKHKVLNVSKAEAGVARILADPTDGYGATNISDVVCNNGD
                     DPEVKKGGTFTCEVVVGGQKRQVLVVFQDDKGTYEVDRPR"
     gene            complement(2013..>2886)
                     /locus_tag="VGP27_18475"
     CDS             complement(2013..>2886)
                     /locus_tag="VGP27_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEV7853318.1"
                     /translation="ADCLGGVYMRWVAEGHSPRFSVNTTRALDQVMAGAIAGRDPIST
                     YDTRSLDPPSHGTALDRVSAIQTGFDLGAQGCAAIDSAEIQQRRGNLPQSLFDPASPQ
                     SDIAINDQTLSTLLELLGQIFSPANPPTLAPEGGGCAVSRQQPVAYCPDTNQIAVDMS
                     TLQKVGAPADESDFVLLQGDNTALSMVTSRYVLALQHERGVSLSSAVAAMRTACLTGV
                     AQRKMADPIRRPSGNQLVLGAGDLDEAVSGLLTNGMVATDVSGTPVPSGFTRILAFRA
                     GLLGDAEDCYQRFA"
BASE COUNT          476 a          943 c          950 g          517 t
ORIGIN      
        1 cgctgagggc gtcgacgaac gcctgtccgg tcgctccgac gccgggagct tcgaaggttc
       61 ctcgggcgaa caccacctcg acgtccgagc atgacgagtc ctcggcggcc gccgccaatc
      121 ctgtcgcctg cggcccgagc aacgccatga tggaaacgaa gactgcacca agccagccca
      181 acgtccgagg gaatgtcgtg gcaacggatt cgcggctgcg catcgtcggc tcttttcggt
      241 tggtcagctc ttaatcgatg agaaccgcct gtacgcgcag attaattccg cagagcccga
      301 agaggttctg gcacacgttc gccgatggac tgcgccgctc cgaggttcct gttgtctcgg
      361 cgcggcgatc aggcaggccg aatccgcctg tgtgacaacg gaaggccatc cctgtgaaag
      421 gcttagccac cgggcatgcg gtaaaaaccg ttgggaaatg cagggtggct ccaatttcct
      481 tcgcacagga tctgcccgga gtcgttgatc cggacagcag agcggatagc gcttttctgg
      541 ccttcgacgc gcgaaccgtg acttagggtc ccgtgacgtg accgaaagaa gtgatcgagc
      601 ccgttcgcga acgttcgttg tgaccggaac ggcatcgggc atcggccttg ctgtcgctga
      661 acgcctactc gccgagggcg gcaccgtcgt cggcgccgat gtgaacccac cgccgaccct
      721 cggtgagcgg ttctctttcg tgacggccga tctttccgac gaatcagcgg tcgccgaggt
      781 tttcgccgcc ctgcccgacc gactcgacgg cgtcgtccat gccgcgggga tcgccagtgg
      841 cggtccggtc catctgctcg acaaggccga gtgggaccgg gtgatcgcga tcaacctcac
      901 cgctaccttc ctcgtggcca aggcggcgct gagtcggatg atcgagcaac cacgggtcga
      961 cagtgagcgc ggctcgatcg tcacccttgc cagcgtcgag ggcctcgagg gaacagcggg
     1021 cggcagcggc tacaacgccg cgaagggcgg ggtcgtgctg ctcaccaaga acattgcgct
     1081 cgactacggg ccgatcggca ttcgggcgaa cgcaatctgc cctggtttca tcgcgacgcc
     1141 gatgttggac agtgtctttc gcatgccggg aatggagcac accttgcggt cgatcaccga
     1201 cgaacacgcc ctgcagcggc tcgggcggcc cgacgagatt gcggccatgg cggccttcct
     1261 cgtgtcgtcc gacgcctcgt tcgtcaccgg ccaggcgatc gccgtcgacg gcggctatac
     1321 cgccggccga gaccacggcg ttgttcggct tttcggcttg cccgattgaa tctcgctgag
     1381 cgccgctgaa gaggtctcta gcgaggtcgg tctacttcgt atgtgccctt gtcgtcctgg
     1441 aacaccacca gcacctgacg tttctggccg ccgacgacca cctcgcaggt gaaggtgccg
     1501 cccttcttga cttccgggtc gtcgccgttg ttgcacacaa cgtctgaaat gttcgtggcg
     1561 ccgtatccgt cggtcgggtc ggcgagtatc cgcgcaacgc cggcttccgc cttggacacg
     1621 ttgagcacct tgtgtttcaa cacgtcgaac cgcgacagca ccaccatcag cagaactgcg
     1681 gcgaccgata tcgcgacgac gccgacggcg atcaacagcg ccaactggcc gctcttctgc
     1741 cgggcggaag tgggacgcgc gtggcgattg cgcgcggggg cgggagcccg accgttgcga
     1801 tcaaccggcg gcttcgcagg tctcggaacg ggtggcggcg acggcgtccg cggcggtgac
     1861 ggaatccgct ggtttgcgtt cttccaccac ggctccacgg ggggcggggc gtctgcactt
     1921 ctggccgacc gcagcggtac ctcgacggtg tcgtcgtcgg acgttggacg gcgcgaccgt
     1981 tggtcgcgcg gtcctgtcac gtgccgattc tctcaggcga accgttgata gcaatcctca
     2041 gcatcgccga gaagtccggc acggaacgcg agaatccggg tgaaccccga cggcacggga
     2101 gttccgctga cgtccgtcgc caccatgccg ttggtcagca ggccggacac cgcttcgtcg
     2161 agatcgcctg caccgagcac caactggttg cccgacggcc gccggatcgg gtcggccatc
     2221 ttgcgctgtg cgacgccggt caggcaggcg gtgcgcatgg ccgcgaccgc cgaactcagt
     2281 gacacgcccc gctcgtgttg cagtgcaagg acatagcgcg acgtcaccat cgacaaggcc
     2341 gtgttgtcgc cctgcagcag gacgaaatcg ctttcgtcgg caggcgcgcc gaccttctgc
     2401 aatgtggaca tgtcgacggc aatctggttg gtgtccgggc agtatgcgac gggctgctgc
     2461 cggctcaccg cgcatccgcc tccctcgggc gcgagtgtcg gcgggttcgc cgggctgaag
     2521 atctgcccca gcagttccaa cagtgtcgac aacgtctggt cgttgatcgc gatgtcgctc
     2581 tgcgggctcg ccggatcgaa aagcgactga ggaaggttgc cgcggcgctg ctggatctcg
     2641 gcggaatcga ttgcggcaca accttgggcg cccaagtcga agcccgtctg gatcgcgctc
     2701 acccggtcga gggcggtgcc gtgcgatggc ggatcgagcg accgtgtgtc ataggtggag
     2761 atcgggtcgc gccctgcgat cgcgccggcc atcacctgat ccaatgcacg ggtggtgttg
     2821 acgctgaacc gcggcgagtg cccctcggca acccagcgca tgtacacccc gcccaggcaa
     2881 tcggcc
//
LOCUS       DASYLX010000792         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3225179,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000792 DASYLX010000000
VERSION     DASYLX010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225179"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1892)
                     /locus_tag="VGP27_18480"
     CDS             complement(312..1892)
                     /locus_tag="VGP27_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853319.1"
                     /translation="MTALNDAERAAIRRDAEGGTDRALPEKVEHSAVAKTSRYPEWLP
                     SRRFIAAVVAIGGMQLLATMDSTVAIVALPKIQDELSLSDAGRSWVITAYVLTFGGLM
                     LLGGRLGDTIGRKRTFIVGVALFTIASILCGIAWDETTLVIARLLQGVGSAIASPTGL
                     ALVATTFPKGPARNAATAVFGAMTAIGSVMGLVVGGALTEVSWRWAFLVNVPVGLMMI
                     YLARTSLRETHKERMKLDASGAILATLGCTAAVFGFSMGPEQGWLTPVTLGSLAAACV
                     FLIAFIIVERSAQNPVVPFELFRDRNRLATFAAIFLAGGVLFTLTVTIGLYVQDILGY
                     SALRAGIGFIPFVLAMGVGLGLSAQLVSMFPPRVLTIAGGVLVLGAMLYGSTLNAGIP
                     YFPNLVAPIVVGGFGIGMIVVPLTISAIAGVGFDQIGPVSAIALMLQNLGGPLVLAII
                     QAVITSRTLYLGGTTGPVKNMDSVQLHALDQGYAYGLLWVAAVAVAVGAVALFIGYTA
                     EQVAHAQEVKDAIDHGDL"
     gene            complement(1926..3122)
                     /gene="cobA"
                     /locus_tag="VGP27_18485"
     CDS             complement(1926..3122)
                     /gene="cobA"
                     /locus_tag="VGP27_18485"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV7853320.1"
                     /translation="MTENAYLVGLQLAGKKVVVVGGGTVAQRRLPLLVATGADVHVVS
                     RSATPAVEAMSGITLTLRPFRDGDLEGAWYVIAATDDPDVNAAVVDEAERQRIFCVRA
                     DAGREGTAVTPATFEYEGLAVGVLAGGEHRRSAAIRSAIHEAFQQGLIAGDAPGVVHG
                     GVALVGGGPGDPELITVRGRRLLAHADVVVADRLAPPELLAELAPHVEVIDAAKIPYG
                     RAMAQDAINAVLIERAREGRFVVRLKGGDPFVFARGYEEVIACTDAGIPVTVVPGVTS
                     AIAVPAMAGVPVTHRSVTHEFVVVSGHVAPGHPESLVNWDALAAMSGTIVLLMAVERI
                     EQFAKVLLDGGRPADTPVLVVQQGTTSAQRTLRTTLTDAPEHIRSEGVRPPAIIVIGP
                     VAAFGG"
     gene            complement(3119..>3523)
                     /locus_tag="VGP27_18490"
     CDS             complement(3119..>3523)
                     /locus_tag="VGP27_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEV7853321.1"
                     /translation="AALAADGAPVHAECAGLTYLVDELEGHPMCGALAGTARFTDSLT
                     LAYRDAAATADSSLYAAGERAVGHEFHRTSVTFTDSYQPAWVYRGSAGAVVRDGAVHR
                     GVHASYLHTHPAARPQAISRFVEAAATSRLAQ"
BASE COUNT          614 a         1229 c         1158 g          522 t
ORIGIN      
        1 ggtgccgtcg agcgtcgaca tcaactgcat gccggtgatc gcgaagatcg cgatgcccag
       61 aacgtgcgtt gtcagattgc ccgcggagcg ctccgtcgac tcgctggaac cacccaccgc
      121 agacatggct tgccagccta cccagactgc ggcggacgac taacgggcgc tatgggtcga
      181 cctgcctaca ggtctcgccg ccccatgtcg acctgtcgac ctacaggtct cgccgcccca
      241 tgtcgacctg cctacaggtc tcgcgcccca tgtcgacctg cctacaggtc tcgcgcccca
      301 tgtcgacctg cctacaggtc tccgtggtcg atcgcgtctt tgacttcctg cgcatgcgcg
      361 acctgctcgg cggtgtagcc gatgaacagc gccacggcac cgacggccac ggccaccgcg
      421 gcgacccaca gcaggccgta cgcgtagccc tggtccagcg cgtgcagttg cacggagtcc
      481 atattcttga ccggccccgt ggtgccgccg agatacagcg tccgcgacgt gatcacggcc
      541 tggatgatgg cgagcaccag cggtccgccc agattctgga gcatcagcgc gatggccgac
      601 accgggccga tctggtcgaa gccgacgccg gcgatcgccg agatggtcaa cgggaccacg
      661 atcatgccga tgccgaatcc gcccacgacg atcggcgcaa cgaggttcgg gaagtacggg
      721 attcccgcgt tgagtgtgga gccgtacagc atggcgccga gcaccagcac gccgcccgcg
      781 atcgtcagca cccgcggcgg gaacatcgac accagctgcg ccgacaaccc gaggccgacg
      841 cccatcgcga gcacgaacgg gatgaagccg atgcccgcgc gtagcgcgct gtagccgagg
      901 atgtcctgca cgtacagccc gatggtcacg gtcagcgtga agagcacacc gcccgcaagg
      961 aagatcgcgg cgaaggtggc cagccggttg cggtccctga acaactcgaa cggcacgacg
     1021 ggattctgcg cggagcgctc gacgatgatg aaggcgatca ggaatacgca ggcggcggcg
     1081 agcgagccga gggtcaccgg cgtgagccag ccctgctcgg ggcccatcga gaagccgaac
     1141 accgcggcgg tgcagccgag cgtcgccagg atggcgccgg acgcgtcgag cttcatccgc
     1201 tccttgtgcg tctcgcgcag gctggtgcgc gccagataga tcatcatcag gccgaccggc
     1261 acgttgacga ggaacgccca ccgccaagaa acctcggtca gtgcgccgcc gaccacgagg
     1321 cccatcaccg agccgatggc ggtcatcgcg ccgaacaccg ccgttgcagc gttgcgggcg
     1381 ggtcccttcg ggaaggtcgt cgccaccagc gcgagacccg tcggcgaggc gatcgccgac
     1441 ccgacgccct gcagaagccg cgcgatcacc aacgtcgtct cgtcccatgc gataccgcag
     1501 aggatcgagg cgatggtgaa cagcgcaaca ccgacgatga aggtgcgttt gcggccgatg
     1561 gtgtcgccga gccgtccgcc cagcagcatc aagccaccga acgtcagcac ataggccgtg
     1621 atgacccaac tgcgacctgc gtcggaaagg ctgagctcgt cttgaatctt aggaagcgca
     1681 acgatcgcga ccgtgctgtc catggtggca agcagctgca tgccgccgat cgccacgacc
     1741 gcggcgatga agcgtcgaga gggcagccat tccgggtacc tgctggtttt cgctaccgcg
     1801 gagtgctcta ccttctccgg aagcgcccga tccgttccgc cttcagcgtc acgtcggatg
     1861 gcggcgcgct ctgcgtcatt gagagccgtc atagctggtt accttacagt aatcttaaga
     1921 tccctttaac cgccgaaggc cgccaccggc ccgatcacga tgatcgcggg cggtcgaacg
     1981 ccctcggatc ggatgtgctc cggcgcgtcg gtcagcgtcg tccgcaaagt tcgctgcgcg
     2041 gacgtcgtgc cctgctggac caccagcacc ggggtatccg caggtcggcc gccatccagc
     2101 agcaccttgg cgaactgctc gatccgctcg acggccatca gcaaaacgat cgtgcctgac
     2161 atcgcggcca gcgcatccca atttactaac gattcgggat gccccggcgc aacgtggccg
     2221 ctgaccacca caaactcgtg cgtcacggaa cgatgtgtga ccggaacgcc cgccatcgca
     2281 ggaaccgcta tggcacttgt cacacccggc accacagtga ccggaatccc ggcgtcggtg
     2341 cacgcgatca cttcctcgta gccgcgcgcg aacacgaacg ggtcgccgcc tttgagacgc
     2401 acgacgaacc ggccttccct ggcccgttcg atgagcacgg cgttgatcgc gtcctgcgcc
     2461 atcgcgcggc cgtaggggat cttcgcggca tcgatgacct cgacgtgcgg ggccagttcg
     2521 gccagaagtt ccggcggtgc gagccggtcg gcgaccacca cgtcggcgtg tgccagaagt
     2581 cgccgccccc gcacggtgat caactcggga tcgccggggc cgccgccgac cagcgcgaca
     2641 ccgccatgca cgacgccggg tgcgtcaccc gcgatcaggc cctgctggaa cgcctcatgg
     2701 atcgccgacc ggatcgccgc ggagcgtcgg tgttcgccgc cggccagcac gcccaccgca
     2761 aggccctcgt actcgaacgt cgcaggggtg accgcggtgc cttcgcggcc tgcgtcggcc
     2821 cgcacgcaga agatccgctg gcgctcggcc tcgtccacga cggccgcgtt gacgtcggga
     2881 tcgtcggtgg cggcgatgac ataccacgcg ccctcgagat caccgtctcg aaaaggcctc
     2941 agcgtcagcg tgattccgct catggcttcc accgcggggg ttgcggacct ggagacgacg
     3001 tggacgtccg caccggtggc gaccagcagc gggagccggc gctgggcgac ggtgccgccg
     3061 ccgaccacca cgaccttctt gccggcgagt tgcaggccga cgaggtaggc gttctcggtc
     3121 actgggcgag cctagaggtt gcggcggcct cgacgaagcg tgatatcgcc tgaggccggg
     3181 ccgccggatg cgtgtgcaga taactcgcgt gcacgccgcg gtgcacggcg ccgtcgcgga
     3241 caacggcgcc cgcggacccg cgatacaccc aggccggctg gtagctgtcg gtgaatgtaa
     3301 ctgaggtgcg gtggaattcg tgtccgacgg cgcgctcccc cgcggcgtaa agcgacgagt
     3361 cggcggttgc ggcggcgtca cggtaggcaa gcgtcaggct gtcggtgaac cgggccgtgc
     3421 ccgcgagcgc gccgcacatg ggatgcccct cgagttcgtc cacgaggtag gtcagacccg
     3481 cgcactccgc gtgcaccggc gcgccgtctg ctgcgagcgc cgc
//
LOCUS       DASYLX010000793         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1360127,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000793 DASYLX010000000
VERSION     DASYLX010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360127"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VGP27_18495"
     CDS             complement(<1..818)
                     /locus_tag="VGP27_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7853322.1"
                     /translation="MTITAPEAVGESLDPRDPVLRLKAFFDDDCTLEFLHERDRSGVL
                     AAAGTVNGVRTVAFCTDGTVMGGAMGVEGCAHIVNAYDTAIEEQSPIVGIWHSGGARL
                     AEGVRALHAVGLVFEAMIRASGYVPQISVVVGFAAGGAAYGPALTDVIVMAPDSRVFV
                     TGPDIVRSVTGEDVDMASLGGPDTHHKKSGVCHIVADDEPDAYARGRRLVGLFCQQGH
                     FDRSKAEAGDTDLHALLPDSPRRAYDVHPLVEALLDSDAPFEEFQAKWAPSMVIG"
     gene            complement(852..2105)
                     /gene="kasB"
                     /locus_tag="VGP27_18500"
     CDS             complement(852..2105)
                     /gene="kasB"
                     /locus_tag="VGP27_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasB"
                     /protein_id="HEV7853323.1"
                     /translation="MSGLATGNGFPNVVVTGVAMTTALATDAEATWKKLLEGQSGIRL
                     LGDAFVEEYDLPVRIGGHLLEDFDGELTRVELRRLSYLQKMSTVMSRRVWKNAGEPDV
                     DPKRLMVSIGTGMGSSEELLFAYEAMRAKGLRGVSPLAVQMYMPNSAAAAVGLERKAR
                     AGVITPISACASGSEGIAHAWRNIVLGEADVAICGGVETKIEAVPIAGFAQMRIVLST
                     TNDDPAGACRPFDKDRNGFVFGEAGALIVIETEEHAKARGANILARLMGASITSDGYH
                     IVAPDPNGEQAAHAMTRAIQLAGLQPSDIDHVNAHATGTSVGDVAEGKAINNALHGHK
                     PAVFAPKGALGHSVGAVGAVESILTMFALRDGVIPPTLNLKNLDPDIDLDVVAGSPRP
                     GNYNYAINNSFGFGGHNVALTFGKY"
     gene            complement(2137..>2733)
                     /gene="kasA"
                     /locus_tag="VGP27_18505"
     CDS             complement(2137..>2733)
                     /gene="kasA"
                     /locus_tag="VGP27_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasA"
                     /protein_id="HEV7853324.1"
                     /translation="RNDDPEGASRPFDKDRDGFVFGEAGALMVIETEEHAKARGAQPL
                     ARLLGAGITSDAFHMVAPAADGLRAGHAMKRSMELAGLEPKDIDHVNAHATATPIGDT
                     AEANAIRVAGVEHAAIYAPKSALGHSIGAVGALESVLTVLSLRDGVIPPTLNYETPDP
                     EIDLDVVAGEPRYGDYKYAINNSFGFGGHNVALAFGKY"
BASE COUNT          456 a          917 c          888 g          472 t
ORIGIN      
        1 ccgatcacca tcgacggggc ccacttggcc tggaactcct cgaagggggc atcggagtcg
       61 agcagcgctt cgacgagcgg gtgcacgtcg taggcacggc gaggcgaatc cggcagcagg
      121 gcgtgcaggt cggtgtcgcc cgcctcggcc ttgctgcggt cgaaatggcc ctgctgacag
      181 aacaatccga ccagccgacg gccgcgcgca taggcgtcgg gctcatcgtc ggcgacgatg
      241 tggcacactc ccgacttctt gtggtgggtg tcgggtccac cgagcgacgc catgtcgacg
      301 tcctcaccgg ttacgctgcg cacgatgtcc gggccggtga cgaacacccg gctgtccgga
      361 gccatcacga tgacgtcggt gagtgcgggg ccgtatgcgg caccgcccgc ggcgaagccg
      421 acgaccaccg aaatctgcgg gacgtaaccg gaagcccgga tcatcgcctc gaacaccaaa
      481 ccgactgcgt gcaatgcccg cacgccctcg gcgagccggg caccgccgga gtgccagatg
      541 ccgacgatcg ggctctgctc ctcgatggcg gtgtcgtagg cgttgacgat gtgcgcgcaa
      601 ccctccacgc ccatcgcgcc acccatcacg gtcccgtcgg tgcagaacgc caccgtccgc
      661 acaccgttca cggtgcctgc ggcggcgagc acgccggagc ggtcccgctc gtgcaggaat
      721 tccagggtgc agtcgtcgtc gaagaacgcc ttcagacgca gaaccggatc gcgcggatcc
      781 agcgattcgc cgactgcctc gggggccgtg attgtcatcg aaacctcctg tcgggacgga
      841 ttatcgggtg ttcagtactt cccgaaggtg agcgccacat tgtgcccacc gaatccgaac
      901 gagttgttga tcgcgtagtt gtaattgccg ggacgcggcg aaccggcgac gacgtcgagg
      961 tcgatgtccg ggtccaggtt cttcaagttg agcgtcggcg ggatgacacc gtcgcgcaac
     1021 gcgaacatcg tcaagatcga ctcgaccgcg ccgaccgcgc cgaccgaatg tccgagcgcg
     1081 cccttgggtg cgaagacggc aggcttgtgg ccgtgcagcg cgttgttgat cgccttgccc
     1141 tcggctacgt cgccaacgct ggtgccggtg gcgtgcgcgt tgacgtggtc gatgtcggag
     1201 ggctgcagac cggcgagctg gatcgcccga gtcatggcat gcgcggcctg ctcaccgttg
     1261 ggatcgggtg caacgatgtg gtagccgtcc gaggtgatgc tggcgcccat cagcctggcc
     1321 aggatgttgg cgccacgggc cttggcgtgc tcttcggtct cgatgacgat cagtgcgcct
     1381 gcctcgccga acacgaatcc gttgcgatcc ttgtcaaacg ggcggcaagc gccggccggg
     1441 tcatcgttgg tggtggacaa aacgattcgc atctgcgcaa atcccgcaat cggtaccgct
     1501 tcgatcttcg tctcgacgcc accacagatc gcgacgtcgg cctcgccgag cacgatgttg
     1561 cgccatgcgt gcgctatgcc ctcggagccg gacgcgcagg ccgagatcgg ggtgatgacc
     1621 ccggccctgg ccttgcgttc cagcccgacc gcggcggctg ccgaattcgg catgtacatc
     1681 tgtaccgcca acggagacac gccacgcagg cccttggccc gcatggcttc gtacgcgaag
     1741 agcagctctt cgctcgaccc catgccggtc ccgatcgaca ccatcagccg tttgggatcg
     1801 acgtcgggct ccccggcgtt cttccacacg cgccggctca tcaccgtcga catcttctgc
     1861 aggtacgaca gccgacgcag ttcgacccgc gtcagctcac cgtcgaaatc ctccagcaga
     1921 tgccccccga tgcgaacggg aaggtcgtac tcctcgacga acgcatcacc cagcaggcgg
     1981 atgccgctct gtccctcgag gagcttcttc cacgtggcct ccgcgtcggt cgccaaagcg
     2041 gtcgtcatgg cgacgcctgt gacgacaacg ttggggaagc cgttccccgt agccagccca
     2101 ctcatgttgc tgcgatggtt ccttcccccc aacgcctcag tacttgccga aggcaagagc
     2161 gacgttgtgt ccaccgaatc caaatgagtt gttgatcgcg tacttgtagt cgccgtaccg
     2221 gggctcgccc gcgacgacgt ccaagtcgat ttccggatcg ggtgtctcgt agttcagtgt
     2281 cggcgggatg acaccgtcgc gcagagacaa gaccgtcagc acggactcga gcgcaccgac
     2341 cgcaccgatg gagtggccga gcgccgactt cggcgcatag atcgcggcat gttccacacc
     2401 ggcgacccga atggcattgg cctccgcggt gtcaccgatg ggggtggccg tggcgtgggc
     2461 gttgacgtgg tcgatgtcct tgggctccag gccggcaagc tccatcgacc gcttcattgc
     2521 gtggccggcg cgcagaccat cggcggcggg cgccaccatg tggaaggcgt cggacgtgat
     2581 gcctgcaccc aagagccggg ccagcggctg tgcgccgcga gccttggcat gctcctcggt
     2641 ttcgatcacc atgagcgccc cggcctcgcc gaacacaaac ccgtcgcggt ccttgtcgaa
     2701 cggacgagaa gcgccctcgg ggtcgtcgtt gcg
//
LOCUS       DASYLX010000794         8343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3225276,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000794 DASYLX010000000
VERSION     DASYLX010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225276"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGP27_18510"
     CDS             <1..621
                     /locus_tag="VGP27_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853325.1"
                     /translation="GHPLEVSHYVSHLMAFFDAIGAERVHLSGESLGGWVASRAAIDH
                     PDRIDRLVLNTAGGSQADPAVMKRIITLSMAAVEAPTWETVQARIKWLMADKTKDYDD
                     IVASRQRIYRQPGFVNAMGDIMALQDPEIRARNLLGPSEYGAISAPTLVVWTSDDPTA
                     DVTEGKRIASMISGARFEMMPGCGHWPQYEDPKTFNRLHLDFLLGR"
     gene            624..2300
                     /locus_tag="VGP27_18515"
     CDS             624..2300
                     /locus_tag="VGP27_18515"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEV7853326.1"
                     /translation="MAERVDIVIVGAGPVGLTLANVLGLQGIRTLVVDERDTLIDYPR
                     GVGLDDEALRTFQSIGLVEQVLPHTVPNQILRFFDSKRKLLAEMAPPDARFGWPKRNG
                     FVQPMVDAELLVGLDRFDNIEVRWGHRMENCTETADGVTVEFADGRDPVSARYVVGSD
                     GGRSATRQLMGVSFDGTTSPTRWLVVDCANDPLGHPNSEVGADPNRPYVSISIAHGIR
                     RFEFMIHGDESDEEADSPAFVRRMLAQMVPHPDRVDIIRHRVYTHHSRIAGAFRHGRL
                     MIAGDAAHLMPVWQGQGYNSGIRDAANLGWKLAAVVNGQAEDALLDTYDVERRKHARA
                     MIDLSTMVGRVISPTNRRLAAVRDKVIHGASLVPTLKRYILEMRFKPMPRYEQGAVFH
                     SEPRAESSPTGTLFIQPRVDTRNRQNVLLDDVLGAGFAVLCWSNNPRALLGDEAFARW
                     KALGASFVAVRPMTQLCWTGHDDDDVVVVGDRTGALKAWFDAHTDSVLFLRPDRCIAG
                     ACIAQRAADVSASLFKVLYLTQGGGNDAADTVLYVPQPTTESSGTVAGSP"
     gene            2248..3168
                     /locus_tag="VGP27_18520"
     CDS             2248..3168
                     /locus_tag="VGP27_18520"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEV7853327.1"
                     /translation="MSHSPLLNLPGPSQDLLDDIDAALADAREFVRAYDPELVVIFSP
                     DHYNGVFYRMMPPFCISTKATGVGDYGTQEGPLDVPADIATDLAEAVLAADVDVAISA
                     SMDVDHGTVQPLQTLFGDAAARPVIPIFVNSVATPLGPLRRSRALGSAVGNYLASLDK
                     RVLVIGSGGLSHDPPVPTLSTAPPAALGRIVLGEPMSPEQRQARQVAVMEAAHNFAHG
                     ESRLQPLNPEWDHRFLEIVDSGHLSDLDDWSTSWMAHEAGHSAHEVRTWVAAFAALAA
                     QGPYTTDNRFYRAAPDLIAGFAVRTAAAAS"
     gene            3168..4928
                     /locus_tag="VGP27_18525"
     CDS             3168..4928
                     /locus_tag="VGP27_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV7853328.1"
                     /translation="MSNEFDTTVDVLVVGSGGGGMTAALTAEAAGLDTLVVEKSSHFG
                     GSTALSGGGIWVPGAPSQRKAGYVPDPDEVLQYLQTITDGLVSHARLRRYVAAAPKMM
                     EFLEGRSRWLEFVWKPGYADYYPELPGGSELGSTINVPAIDLRRLGPEEQNLLAPLAL
                     APKGIWFAPKDLRLFYQVRQNWRGKAVLLKLIWRMFRARVFGDRMAAIGQSLAARLRM
                     AMKEHDVALWLDSPMTELITDVDGAVVGAVVERDGKQVRIGARGGVILAAGGFDHDME
                     WRRQHLPVLEKDWSFGNPASTGDGIRAGEKAGGSTDLLDEAWWFPAMCWPDGRLQFML
                     NERMMPPQFVVNGDGNRFINEAAPYMDFAHAMIEGQNSGVAHIPCWLITDIRSFHRYV
                     VGGHLPIPKVPFAPVPTGRKVPKAWLESGIVKQANSFEELATQIGVPPAQLRRTADRF
                     NELAHKGHDDDFNRGDSKYDNYYGDPTLPNPNLHPLGKPPYYAFQIILGDLGTSGGLR
                     TDEFARVLRSDDSPVKGLYAVGNTSAAVMGRSYAGAGATIGPAMTFGYVAAKHIEDQL
                     PETGGSKTDSTLDSHRKVTK"
     gene            4925..6265
                     /locus_tag="VGP27_18530"
     CDS             4925..6265
                     /locus_tag="VGP27_18530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7853329.1"
                     /translation="MKISLFYEFPLPRPWSDDDEHKLFQDGLDEVEAADKAGFSTVWL
                     TEHHFLEEYCHSTAPEIFLSAASQRTKDIRLGFGIMHLPPQVNHPARVAERVSTLDLV
                     SNGRVEFGTGESSSVGELGGFGIDPADKRKMWEEALEVSIRCMIEEPFTGFKGEHVEM
                     PPRNVVPKPLQKPHPPVWVACTRPASVSMAAQKGLGALSFAYTGPGPLTERVNGYYKE
                     FEETAVPVTPLMNPNILAIGGDLSMMVAPTDEQAIERLGKGGGFFAFGIMHYYMTGMH
                     TPGRTGVWKRHLEEIEKDPSVVYGPDRGPIGSPATVREFLRGYEESGVDELILLLTPR
                     RHEETMESIELMGSEVLPEFIERHEKSAAEKAKRMEPILEKAEARRPASNAPTFDETY
                     SFGGLPTGRENYTANEVSLAMDEMNAGIEAAAAKLKSGAGWDSRNPTADARSTE"
     gene            6378..7220
                     /locus_tag="VGP27_18535"
     CDS             6378..7220
                     /locus_tag="VGP27_18535"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HEV7853330.1"
                     /translation="MAQIDDLWRYDGRRAVVTGCSSGIGAEVARQLTELGADVVGLGL
                     ERPALQLKEFHEVDLSDSASIDRAAAAIGGQVDALFNIAGVSSGIGNPLLVVTINFLG
                     MRHLTEALIPSMPPGSAIANVSSLAAAEYRANAEVTLGLLDTATMAEGIRWCEQNPEP
                     VADGAGYRLSKEATILYGMANVVALGAKGIRINCTAPGVTDTPILDQLRSAFGQEFVD
                     SFTTPLGRVAAPDEQASVLVFLNSAAARYITGEVICVDGGAVAESFFGDVSDRRKVER
                     ARAE"
     gene            7204..8193
                     /locus_tag="VGP27_18540"
     CDS             7204..8193
                     /locus_tag="VGP27_18540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV7853331.1"
                     /translation="MPGLSDFRRVADDVRNWGRWGDADELGTLNLITAEKVQQAASLV
                     RHGKVFPLGVDFGSSGPQGAFQYRQNPTHVMTVDGGDAATLLKFGPTWLKNPAAVQLS
                     SYSEAGPMRFNDDMIIMPLQAATQWDALSHVYYEDKLYNGFPADSVTSLGAFYCGIDK
                     VDGKGITSRGVLLDIVALRGVETYCELGDPITPAELDEAARAQGVTVERGDVVLVRTG
                     WWARFLQTGDGAEPGAGLDWTCASWLHDHEVAAVAADNLMVENPVSAVDGAILPMHML
                     CLRDMGLMLGEYWDLTALAEDCAADGVYEFQLIAPPLRVTGAVGSPVNPIAIK"
     gene            8203..>8343
                     /locus_tag="VGP27_18545"
     CDS             8203..>8343
                     /locus_tag="VGP27_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEV7853332.1"
                     /translation="MTATTTGGAPLVVGLGGTLRANSSTERALRYCLASVESQGGQTR
                     LFS"
BASE COUNT         1461 a         2705 c         2802 g         1375 t
ORIGIN      
        1 ggccacccgc tcgaggtcag ccactacgtc tctcacctga tggcgttctt cgacgccatc
       61 ggcgccgaac gggtgcatct gagcggggag tcgctcggcg ggtgggtggc gtcgcgcgcc
      121 gcgatcgacc accccgaccg catcgatcgg ctggtgctca acaccgcggg cggttcgcag
      181 gccgatcccg cggtgatgaa gcgaatcatc acgctctcga tggcagcggt cgaagcgccg
      241 acctgggaga ccgtgcaggc ccggatcaag tggctcatgg ccgacaaaac caaggattac
      301 gacgacatcg tcgccagcag gcagcggatc taccggcagc ccgggttcgt caacgccatg
      361 ggcgacatca tggcgctgca ggatccggag atcagggcgc gaaatctgct ggggccaagc
      421 gaatatgggg cgatcagcgc gcccacgctc gtcgtgtgga ccagtgacga cccgaccgcc
      481 gatgtcaccg aaggcaagcg catcgcgtcg atgatttccg gggcgcgctt cgagatgatg
      541 cccggttgtg gccactggcc gcagtacgag gatccgaaga cgttcaaccg gctccatctg
      601 gacttcctgt tggggcgctg acagtggctg agagggtgga catcgtcatc gtcggggcgg
      661 gcccggtggg gctgaccttg gcgaacgtcc ttgggctcca gggaatccgc acgctcgtgg
      721 tggacgagag ggacaccctc atcgactacc cgcgcggcgt cgggctcgac gacgaggcgc
      781 tgcggacgtt ccagtcgatc gggctggtgg aacaggtgct gccccacacg gtgcccaacc
      841 agatcctgcg tttcttcgac tcgaagcgga agctgcttgc ggagatggca ccgcccgacg
      901 cccgcttcgg gtggcccaag cgcaacgggt tcgtccaacc catggtggac gccgaactgc
      961 ttgtcggcct tgaccgcttc gacaatatcg aagtgcggtg gggccaccgg atggagaact
     1021 gcactgaaac ggccgacggc gtgacggtcg aattcgccga cggtcgtgac ccggtctccg
     1081 cgcgttacgt ggtcggcagc gacggcggtc gcagcgcgac ccggcagctg atgggggtgt
     1141 cgttcgacgg tactacctcc ccgacccgct ggctggtcgt cgattgtgcg aacgacccgc
     1201 tgggccatcc caacagcgag gtgggagccg atcccaaccg tccatacgtg tcgatatcga
     1261 tcgcccacgg tattcggcgg ttcgagttca tgattcacgg tgacgagtcc gacgaagagg
     1321 cggacagccc ggcgttcgtc cggcgcatgc tggctcaaat ggttccccat cccgaccggg
     1381 tcgacatcat ccggcaccgg gtctacacgc atcactcgcg gatcgcgggc gcgttccgac
     1441 acgggcggtt gatgatcgca ggcgacgccg cccatttgat gccggtgtgg cagggccagg
     1501 gctacaacag cggcatccgc gacgccgcca acctcggctg gaagctcgcg gcggtggtga
     1561 acggccaggc cgaggacgcg ctactcgaca cctacgacgt cgaacgacgc aagcatgcgc
     1621 gcgcgatgat cgacctgtcc acgatggtcg gacgggtgat ttcgccgacg aaccgacggc
     1681 tggcggcggt gcgcgacaag gtgattcatg gtgcgtcgct ggttcccacc ctcaagcgct
     1741 acatactcga gatgcggttc aagccgatgc ctcgctacga acagggcgcg gtcttccatt
     1801 ccgaaccgcg cgctgagtcc tccccgacgg gaacgctgtt cattcagccc cgcgtggaca
     1861 ctcgcaatcg gcagaatgtg ctactcgacg acgtgctcgg cgccggcttc gccgtgctgt
     1921 gctggagcaa caacccgcgc gcgctgctcg gcgatgaggc attcgcgcga tggaaggctc
     1981 tgggagccag cttcgttgcg gtacggccga tgacgcagct gtgctggaca gggcatgacg
     2041 atgacgacgt tgtcgtcgtc ggtgaccgca cgggtgcact caaagcctgg ttcgacgcgc
     2101 acaccgactc cgtgctgttc cttcggcccg atcggtgcat cgcaggcgcg tgcatcgctc
     2161 aacgcgcagc cgacgtgagc gcgtcgcttt tcaaggttct ctatctgacc cagggaggag
     2221 gcaacgatgc cgctgacact gtgctgtatg tcccacagcc cactactgaa tcttccggga
     2281 ccgtcgcagg atctccttga cgacatcgac gcagccctcg ccgacgccag ggagttcgtg
     2341 cgtgcctacg accccgagct cgtcgtcatc ttctccccgg accactacaa cggcgtcttc
     2401 taccgcatga tgccgccgtt ctgcatcagc acgaaggcga ccggggtcgg cgactacggc
     2461 acccaggagg gtccgctcga cgttcccgcc gacatcgcca ccgatctggc cgaggcggtg
     2521 ctcgccgcgg acgtcgacgt cgcgatctcg gccagcatgg acgtcgatca cggcacggtg
     2581 cagccgctgc aaaccctgtt cggtgacgcc gcggcccggc cggtcatccc gatcttcgtc
     2641 aactcggtcg ccacaccgct tgggccactt cgtcgttcgc gcgcgctggg cagcgcggtc
     2701 ggtaactacc tggcgtcgct ggacaagcgg gtgctcgtca tcggatcggg tgggctgtcc
     2761 cacgatccgc ccgtccccac gctctcgacg gcaccgcccg cggcgctcgg ccgaatcgtg
     2821 ctcggcgagc cgatgtcgcc ggagcagcgt caggcgcgtc aggtcgcggt gatggaggcg
     2881 gcgcacaact tcgcccacgg cgaaagcagg ctgcagcccc tcaacccgga gtgggatcac
     2941 cgcttcctgg aaatcgttga cagcggccac ctctccgacc tcgacgactg gtccacgtcg
     3001 tggatggcgc acgaggcggg ccactcggcg cacgaggtcc gtacatgggt ggccgcgttc
     3061 gccgcactcg ccgcgcaggg gccgtacacg accgacaacc ggttctaccg tgccgctccg
     3121 gacctgatcg cgggattcgc cgttcggacg gcggcggccg cttcgtgatc tccaacgaat
     3181 tcgacacgac ggtcgacgtc ctcgtcgtcg gctcgggtgg cggcggcatg accgccgcgc
     3241 tcaccgcaga ggcggccgga ctggacacgc tcgtcgtcga aaagtcttcc cacttcggcg
     3301 gatccaccgc actctccggc ggcggcatct gggtgcccgg agcgccgtcg caacgcaagg
     3361 cgggctacgt ccccgacccc gacgaggtcc tccagtacct gcagacgatc accgacggtc
     3421 tcgtcagcca cgctcggcta cgtcggtatg tggccgcagc gccgaagatg atggagttcc
     3481 tcgaggggcg cagccgttgg ctcgagtttg tctggaagcc gggctacgcc gactactacc
     3541 cggagctacc cggcggttct gagctcggca gcacgatcaa cgtgcccgcc atcgacctgc
     3601 gcaggctcgg cccagaagag cagaacctgc ttgcgccact cgcgttggca ccaaagggaa
     3661 tctggttcgc cccgaaggat cttcggctgt tctaccaggt ccgccagaac tggcgcggta
     3721 aagccgttct gctgaagctg atctggcgga tgttccgggc gcgggtgttc ggcgatcgga
     3781 tggcggccat cgggcagtcg ctcgccgcgc ggctccggat ggcgatgaag gagcacgacg
     3841 tcgcgctgtg gctcgactcc ccgatgacgg agttgatcac cgatgtcgac ggcgcggtcg
     3901 tcggcgcggt cgtggaacgt gacggcaagc aagtgcggat cggcgcacgc ggcggcgtga
     3961 tactcgccgc aggcgggttc gaccacgata tggagtggcg cagacagcac ctgcccgtgt
     4021 tggagaagga ctggagcttc ggcaatccag cctccacggg tgacggcatc cgcgcggggg
     4081 agaaggcagg cggatccacc gaccttctcg acgaggcatg gtggtttccc gccatgtgct
     4141 ggcccgacgg acgccttcag ttcatgctca atgaacggat gatgcccccg cagttcgtcg
     4201 tcaacggtga cggcaaccgc ttcatcaacg aggccgcgcc gtacatggac ttcgcccacg
     4261 cgatgatcga gggacagaac tcgggtgtcg cgcacatccc gtgctggttg atcaccgaca
     4321 tccggtcgtt ccaccggtac gtggtcggtg ggcatctgcc gattccgaag gtgccgttcg
     4381 cgccggtgcc cactgggcgg aaggtgccga aagcgtggct cgagtcgggt atcgtcaagc
     4441 aagccaacag cttcgaagaa ctcgcgaccc agatcggcgt gccgcccgca cagcttcgcc
     4501 ggacggccga ccgcttcaac gagttggcgc acaagggcca cgacgacgac ttcaaccggg
     4561 gcgattcgaa gtacgacaac tattacggcg accccacact gcccaatccc aacctgcacc
     4621 cgctaggtaa gccgccgtac tacgcgttcc agatcatcct cggcgacctc ggcacgtcgg
     4681 gcgggttgcg gaccgacgaa ttcgcccgcg tgctgcgcag cgacgacagc cccgtcaaag
     4741 gcctttatgc ggtgggcaac acctcggccg cggtgatggg tcgcagctac gcaggcgcgg
     4801 gtgcgaccat cggccccgcc atgaccttcg gctacgtggc cgccaagcac attgaagacc
     4861 agctgccgga aaccggtggc agcaagaccg attcgacact cgattcgcat cgaaaggtaa
     4921 ccaaatgaag atctcgctgt tctacgagtt cccgctgccg cggccgtgga gtgacgacga
     4981 tgagcacaag ctcttccagg acggcctcga cgaggtcgag gcggcggaca aggcagggtt
     5041 ctcgacggtg tggctgaccg agcaccactt cctcgaggag tactgtcact ccaccgcgcc
     5101 ggagatcttc ctgtccgcag ccagccagcg caccaaggac attcggctcg ggttcgggat
     5161 catgcacctg ccgccgcagg tcaatcatcc ggctcgggtc gccgagcggg tgtcgacgct
     5221 ggacctggtc tccaacggtc gcgtggagtt cggcaccggc gagtcgtctt cggtgggtga
     5281 gctcggcgga ttcggcatcg atcccgcgga caagcgcaag atgtgggagg aagcactcga
     5341 ggtctccatc cgctgcatga tcgaggaacc gttcaccggc ttcaagggtg agcatgtcga
     5401 gatgccgccg cgcaacgtcg ttcccaagcc gctgcagaaa ccgcacccgc cggtgtgggt
     5461 ggcgtgcacc aggcccgcgt cggtgtccat ggccgcgcag aagggcctcg gcgcactgag
     5521 tttcgcctac acgggccccg gcccgctgac ggagcgggtc aacggctact acaaggagtt
     5581 cgaggaaacc gccgtgccgg tcacgccgct gatgaacccc aacatcctcg cgatcggcgg
     5641 cgacctgtcg atgatggtgg cgcccaccga cgagcaggca atcgagcggc tcggcaaggg
     5701 cggcgggttc ttcgcgttcg gaatcatgca ctactacatg accggcatgc acactcccgg
     5761 ccggaccgga gtgtggaagc gccatctcga ggagatcgag aaggatccca gcgtcgtcta
     5821 cggcccggac cgtggcccga tcggcagccc cgccaccgtg cgtgagttcc tgcgtggcta
     5881 cgaggagagc ggggtcgacg agctgattct gctgctgacc ccgcgtcgtc acgaggagac
     5941 gatggagtcc atcgagctga tgggctccga agtcctgccc gagttcatcg agcgccacga
     6001 gaagtcggcc gccgagaagg cgaagcggat ggagccgatc ctcgagaagg cagaagcccg
     6061 caggccggcg tcgaatgcgc cgacgttcga cgagacgtat tcgttcggcg ggctgccgac
     6121 cggtcgcgag aactacaccg ccaacgaggt gtcgctggcg atggacgaga tgaacgcagg
     6181 catcgaagcg gctgcggcga agctcaagtc gggtgcaggc tgggacagca ggaaccccac
     6241 cgcggacgcg aggagcactg aatgacttcg cggacgcgag gagcactgaa tgactccgcg
     6301 gacgcgagga gcactgaatg acttccgcgg acgcgaggag cactgaatga cttccgcgga
     6361 caagagatag aggaaacatg gcgcagatcg acgacctctg gcgttacgac ggccgccgcg
     6421 cggtggtcac cggctgcagc tcgggcatcg gtgccgaagt agcgcgccaa ctgaccgagc
     6481 ttggcgccga cgtcgtcggg ttgggtctgg agcggcccgc gctgcagctc aaggagttcc
     6541 atgaagtcga cctgtccgac tcggcgtcga tcgacagggc cgccgccgcg atcggcggcc
     6601 aggtggatgc cctcttcaac atcgccggtg tgtcgtcggg catcggaaac ccgttgctgg
     6661 ttgtgacgat caatttcctc ggcatgcggc acctcaccga ggcgctgatc ccgtcgatgc
     6721 cgcccggatc ggcgatcgcc aacgtttcct cgctcgccgc ggcagagtac cgggccaacg
     6781 cagaagtcac cttggggctg ctggacaccg ccacgatggc agaaggcatc cggtggtgtg
     6841 agcagaatcc cgagccggtc gccgacggcg cgggctatcg gttgtccaag gaggcgacca
     6901 tcctctacgg gatggccaac gtcgtcgcac tcggcgccaa gggcattcgg atcaactgca
     6961 ccgcaccggg cgtcaccgac acgcccatcc tcgaccaact tcgctccgcg ttcggtcagg
     7021 agttcgttga ttcgttcacg acccccctcg gccgtgtcgc ggctccggac gagcaggcga
     7081 gcgtgctggt gttcctcaac agtgccgcgg cgcgatacat caccggtgag gtgatctgcg
     7141 tcgacggcgg tgccgtggcg gagagcttct tcggcgacgt ctcggacaga cgaaaggtgg
     7201 aacgtgcccg ggctgagtga ctttcgtcgc gttgccgacg acgtccgcaa ctgggggcgg
     7261 tggggtgacg ccgacgaatt gggcacgctg aacctgatca ccgccgagaa ggtccaacaa
     7321 gcggcgagcc tggtgcggca cggcaaggtc tttccgttgg gcgttgactt cggctcgtcg
     7381 ggaccgcagg gcgcgttcca gtaccggcag aacccgacgc acgtgatgac ggtcgacggc
     7441 ggcgacgcgg cgacacttct gaagttcggt cccacttggc tgaagaatcc ggctgccgtg
     7501 cagttgagca gttactccga ggcaggcccg atgcgcttca acgacgacat gatcatcatg
     7561 ccgttgcagg cggccaccca gtgggacgcg ctgtcccacg tctactacga ggacaagctg
     7621 tacaacgggt tcccggcgga ctcggtgacg agcctcggcg cgttctactg tggcatcgac
     7681 aaggtggacg gcaagggcat cacgtcgcgc ggtgtgctgc tcgacatcgt cgcgcttcgc
     7741 ggggtggaga cctactgcga actcggcgac ccgatcaccc ctgccgagct cgacgaggcg
     7801 gcccgcgcac agggcgtgac cgtcgagcgt ggtgacgtcg ttttggtccg aacaggttgg
     7861 tgggcaaggt ttttgcagac cggcgacggt gcggaaccag gtgcggggct ggactggacg
     7921 tgtgcgtcgt ggctgcacga ccacgaggtg gccgcggtcg ccgccgacaa cctgatggtc
     7981 gagaaccccg tctccgccgt agacggcgcg atcctgccca tgcatatgct gtgcctgcgc
     8041 gacatggggc tgatgctcgg cgagtactgg gacctgacag cactcgccga ggactgtgcc
     8101 gccgacgggg tgtacgaatt ccaactcatc gcgccgccgc tgcgggtaac cggggctgtc
     8161 ggatcaccgg tgaatcccat tgcaatcaag taggtgtcaa ctatgactgc aaccacaacg
     8221 ggtggtgctc cgctcgtcgt cgggctcggc ggaacgctgc gggccaactc gtcgacggaa
     8281 cgggcgctgc gctactgcct ggcctccgtc gaatcgcagg gcggacagac gaggctgttc
     8341 agt
//
LOCUS       DASYLX010000795         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_154543,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000795 DASYLX010000000
VERSION     DASYLX010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154543"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGP27_18550"
     CDS             complement(<1..434)
                     /locus_tag="VGP27_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887496.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7853333.1"
                     /translation="MEVLPGYTVGQVLEAFWTTIQLTVFSAVGALILGTALAAMRLAP
                     VPMLNWLGTTYVNVVRNTPLTLIILFCSFGLAQTLGVTFVDSNSPTSIEDSNFRLAVL
                     GLTVYTASFVCETVRSGVNTIPLGQAEAARSLGLSFGQNLRI"
     gene            complement(459..1274)
                     /locus_tag="VGP27_18555"
     CDS             complement(459..1274)
                     /locus_tag="VGP27_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7853334.1"
                     /translation="MASFSLRVAGAVALAMALPFAATACGGGGGGENSIVIGTKFDQP
                     GLGLKNPDGKMSGFDVDVATYVAKELGYPADKIEWKESPSGQRETLIQNDQVKFIAAT
                     YSITDARKEKVSFAGPYLVTGQSLLVKADNNDITGAESLQNNKKLCSVSGSTPAQRIK
                     DKYPGVQLQQYDTYSACVEALKNGAVDAVTTDEVILAGYAAQSPGAFKIVGKPFSQEN
                     YGIGLKKGDTELCTKITDAVKKMESEGAWKAAWDKNLGPAGIAAPAPPAVAPC"
     gene            complement(1308..>1761)
                     /locus_tag="VGP27_18560"
     CDS             complement(1308..>1761)
                     /locus_tag="VGP27_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853335.1"
                     /translation="TILENVALAPRKVRKASKEDARKKAMELLDRVGVANQADKYPAQ
                     LSGGQQQRVAIARSLAMNPKVMLFDEPTSALDPEMISEVLSVMTSLAKDGMTMIVVTH
                     EMGFARGAANRVVFMSDGAIVEAAEPKEFFENPRSDRAKDFLGKILKH"
BASE COUNT          277 a          567 c          554 g          363 t
ORIGIN      
        1 attattcgga ggttctggcc gaatgacaat cccagcgacc gcgccgcctc ggcctggccc
       61 aacgggatgg tgttgacccc ggatcgtacc gtctcgcaga cgaatgacgc tgtgtagacc
      121 gtcaacccga gtacggcgag ccggaagttg ctgtcctcga tcgatgtcgg tgaattcgag
      181 tcaacgaacg tgacgcccag cgtctgcgcc agaccgaacg agcagaacag gatgatcagc
      241 gtcagtgggg tgttgcgcac cacgttgaca taggtcgtcc cgagccagtt gagcatcggc
      301 accggtgcca gccgcatcgc ggccagcgcc gtaccgagga tcagcgcccc gacggcggag
      361 aacaccgtta gctgaatcgt cgtccagaag gcctcgagaa cctggcccac ggtgtatccg
      421 ggtaggacct ccacgtgcgg ttggtcagct ctgccgggtc agcagggggc gacagcgggc
      481 ggcgcggggg cggcgatacc cgcgggaccg aggttcttgt cccacgcggc cttccacgcg
      541 ccttccgact ccattttctt caccgcgtcc gtgatcttgg tgcacagttc cgtgtcaccc
      601 tttttcaggc cgatgccgta gttctcctgc gagaacggct tgccgacgat cttgaacgcg
      661 ccggggctct gtgcggcata accggcaagg atcacctcgt cggtggtgac cgcgtcgacg
      721 gccccgttct tcagcgcctc gacacaggcc gagtacgtgt cgtactgctg tagctgcaca
      781 cccgggtact tgtccttgat gcgctgcgcg ggcgtcgaac ccgacaccga gcacagcttc
      841 ttgttgttct gcagcgactc ggcgccggtg atgtcgttgt tgtcggcctt caccaggagg
      901 ctctggccgg tgaccaggta ggggcccgcg aagctgacct tctccttgcg ggcgtcggtg
      961 atcgagtagg tggcggcgat gaacttgacc tggtcgttct ggatcagggt ctcgcgctgg
     1021 cccgacggcg attccttcca ctcgatcttg tctgcgggat agccgagttc cttggcgaca
     1081 taggtggcga cgtcgacgtc gaagccgctc atcttgccgt cgggattctt caggccgaga
     1141 ccgggctggt cgaacttcgt cccgatgacg atcgagttct cgccgccgcc gccgccacca
     1201 catgcagtgg ccgcgaacgg cagcgccatg gccagtgcta cggctcctgc gacacgaagt
     1261 gagaaggacg ccattttgtt gttcctttcg ccggggcttt gttgctctca gtgcttgagg
     1321 atctttccga ggaaatcttt ggcgcggtcc gagcgggggt tttcgaagaa ttccttcggc
     1381 tcggcggctt cgacgatcgc gccgtcggac atgaacacca ctcggttggc cgcaccgcgg
     1441 gcgaacccca tttcatgggt caccacgatc atcgtcatgc cgtccttggc cagcgaggtc
     1501 atcaccgaca gcacctcgct gatcatctcc gggtccagcg cactggtcgg ctcgtcgaac
     1561 agcatgactt tcgggttcat cgccagcgac cgcgcgatcg ccacccgctg ctgctgcccc
     1621 cccgatagct gtgcggggta cttgtcggcc tggttggcca cgccgacgcg gtccagcagc
     1681 tccatcgcct tcttgcgcgc gtcctccttg gacgctttgc gcaccttgcg gggtgccagt
     1741 gcgacgtttt ccagaatcgt c
//
LOCUS       DASYLX010000796         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3776248,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000796 DASYLX010000000
VERSION     DASYLX010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3776248"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGP27_18565"
     CDS             <1..394
                     /locus_tag="VGP27_18565"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7853336.1"
                     /translation="GDAVMVTFNVSADQPDHAVRAARAAVDFQAAARRVADQHPGWPR
                     FRAGINTGSASVGVVGDGRQRAYTVLGDTVNVAAHIEASAPVGAIAISDATYRALTDA
                     DVTSLGIVTLKTRAEPVQIWRLDGLSSS"
     gene            complement(407..685)
                     /locus_tag="VGP27_18570"
     CDS             complement(407..685)
                     /locus_tag="VGP27_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEV7853337.1"
                     /translation="MKRYVIEREIPGASDLSHEELKAIACKSNDVVESLGVPYRWITS
                     YVAGDKIYCVHESDDEDAVREHARRGGFPANSVNVIANEFGPHTAGSQ"
     gene            846..3464
                     /locus_tag="VGP27_18575"
     CDS             846..3464
                     /locus_tag="VGP27_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7853338.1"
                     /translation="MVLAERDHQLGAIIARADDARAGRGGMVFVSGESGAGKTSFVES
                     FVERWVCDERVLWGACDPLPTPRPLGPIHDVAHRLAPATQAVLAQSEAPYDIFEAVYN
                     DLRTAPSVLVLDDLQWADQGSIDLLRFVLRRVAQTRSLAVGILRDDEVGVGHPMRVLL
                     GDAARSARAASLPLPPLSVAGVDRLVGERAVDSAWLHRITGGNAFFVCEMLEHQHAPE
                     MDLPTTVRDAVLARTTDLDASAWDALNLLTCSPGAVPDRLLTDLGVTLPALRALSDAG
                     LIRRSSRGVAFRHDLCRMAISSVIPPGAEAGLHRRLLDAYEATSAVDPAVLTHHALGA
                     GDIERIRRAAYDAGVASARSGAHTQAAEFFTIALDHGGLSDPHSEAELLELLAWEFYL
                     IDRLPEAISTCGRAMRIRRELGESAAVSANHHSLSVYHWYSANRELAEQHAAEASQVL
                     DVDTDDVDELVALGQAFAMQAYLAGHASLLDVATTFISRAHEIADRTGDPDLTVRVRL
                     IENYGVILMGDESGRDAILSILQSAPKHIDELYSGGWSCITYFDVEQRRLDLAAELLD
                     VSIPLMLEHDLPICRVWQMGSRARMHLMVGEWDDATADADRVLDAPSAPLARTWPLLI
                     RALVALRRRGQGADSLDEAWQLACRFGEPIRMLPAAAAIAESCWLTGVPDDRLMQCRK
                     LLADVPAEGLEWSRGELGAWLCRLGDTVDTADLAAPYRLLLQGSHEAAAEEFHRLSMP
                     YDAALALLDSDDLSLTGRALDILDRLGADAVAAKVRRGLRARGESAVPARRRTATLAN
                     PAGLTARQVEVLRLLDDGLTNVELAERLYLSVKTVDHHVSAILAKLEVNKRRDAVRKA
                     RKLGILADSAPVEVPL"
     gene            complement(3438..3902)
                     /locus_tag="VGP27_18580"
     CDS             complement(3438..3902)
                     /locus_tag="VGP27_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853339.1"
                     /translation="MIVERQSIVGAPTERVWARVVTPEGINDELRPWMTMSMPRGRES
                     LTVDAIPVGIPVGRCWMRLFGVLPFDYDDLLIAELWPGRGFHEESTMLSMRLWRHERT
                     LERDRDTKAVVRDRLTFELRWPLRLLTPIVAAGIRALFGQRQRRLQRYFDGR"
BASE COUNT          597 a         1318 c         1331 g          676 t
ORIGIN      
        1 cggggacgcc gtgatggtga ccttcaacgt ctccgccgac cagcccgacc acgccgtcag
       61 ggccgcgcgg gccgccgtcg acttccaggc cgccgctcgg cgcgtcgccg accagcatcc
      121 gggctggccg aggttccggg ccgggatcaa taccggttca gccagtgtcg gtgtggtcgg
      181 cgacggcagg cagcgggcct acacggtgct cggcgacact gtgaacgtcg ctgcccacat
      241 cgaagcgtcg gcgccggttg gcgcgatagc gatcagcgat gcgacgtatc gtgcgctgac
      301 cgacgccgac gtcacctcgc tgggcatcgt caccttgaag acgagggccg aacccgtaca
      361 gatctggcgg ctcgacggtc tgagttcttc atgaccccaa ggagattcac tgcgacccgg
      421 cggtgtgcgg tccgaactcg ttggcgatga cgttgacgct gttggccgga aagccgccgc
      481 ggcgggcgtg ctcgcggacg gcgtcctcgt cgtcggactc gtgcacgcag tagattttgt
      541 cgcctgcgac gtaactggtt atccagcggt acgggacgcc gagcgactcg acgacgtcgt
      601 tcgacttgca ggcgatggcc ttcagctcct cgtggctgag gtcactggct ccgggaatct
      661 cccgttcgat gacatagcgt ttcatgggtc tctcgctcct gtgtggcgtc tgccgcgacg
      721 atctgtcggg cagcaccaac ggtgcggcac cggttggacc ggcacatggg gagaaagtcc
      781 ccatcttggg tcgtggcgga tgtttcagct gatcgcaggg gtaaaccgca ctaccatcac
      841 ttctcgtggt gctcgccgaa cgggatcatc agctcggcgc gatcatcgcc agggccgacg
      901 acgcgcgggc cggccgcggc ggcatggtgt tcgtgtcggg cgaatccgga gccggaaaga
      961 cctcgttcgt cgagtcgttc gtcgagcgct gggtctgcga cgagcgcgtg ttgtggggtg
     1021 catgcgaccc gctgccgacc ccgcgaccgc tcggtccgat tcacgacgtg gcccaccggc
     1081 ttgcacccgc cacccaggca gtgctggccc agagcgaggc gccctacgac attttcgagg
     1141 ccgtctacaa cgatcttcgc accgcgccgt cggtccttgt cctcgacgat ctgcagtggg
     1201 ccgaccaggg ctccatcgac cttctccggt tcgtgctgcg gcgcgttgcg cagacccgct
     1261 cgcttgctgt cggcatcctg cgggacgacg aggtgggtgt cggccaccct atgcgcgtgc
     1321 tgctcggcga tgcggcgcgt tcggcccgcg cagcatcact gccgctgccg ccgctcagtg
     1381 tggccggcgt tgaccgactg gtcggtgagc gtgccgtaga ttccgcgtgg ctgcatcgaa
     1441 tcaccggagg caacgccttc ttcgtgtgcg agatgctcga gcaccaacac gcgccggaaa
     1501 tggatctgcc cacgaccgtc cgggacgcgg tgctggcccg caccaccgat ctcgacgctt
     1561 ccgcctggga cgcgctgaac ctgctgacgt gttcgccggg cgcggtaccc gaccgcctgc
     1621 tcaccgatct gggtgtgacg ctgcccgcgc tgcgcgcgct gtccgacgcc ggcctgatcc
     1681 gtaggagttc acgcggcgtc gccttccgcc acgacctctg ccgcatggcc atcagcagcg
     1741 tcatcccgcc cggcgccgag gcgggtctgc accggcggct gctggacgcg tacgaggcga
     1801 cgtcggccgt cgacccggcg gtgctcaccc accacgcgct cggcgcgggc gacatcgagc
     1861 gaatcaggcg ggccgcctac gacgccggcg tcgcgtcggc ccggtccggc gcacacaccc
     1921 aggccgccga attcttcacg atcgcactcg atcacggcgg actgtccgat cctcacagcg
     1981 aggcagagct gctggagtta ctcgcctggg agttctatct gatcgacaga ctgcccgaag
     2041 cgatcagtac ctgcggtcgc gcgatgcgca tccgacggga gctcggcgag tcggcggcgg
     2101 tcagcgcgaa tcatcactcg ctgtcggtct atcactggta cagcgccaac cgggagctgg
     2161 ccgagcaaca tgcggccgaa gcgagtcagg tgctcgacgt cgacaccgac gacgtggacg
     2221 aactcgtggc gctcggccag gccttcgcca tgcaggccta cctcgcgggc catgcgagcc
     2281 ttctggacgt tgccacgacc ttcatctccc gcgctcacga aatagccgat aggaccggcg
     2341 atcccgatct aacggtacgt gtccggttga tcgagaacta cggcgtcatc ctgatgggcg
     2401 acgagtcggg gcgggacgcg atcctctcga ttctgcagtc cgcgcccaag cacatcgacg
     2461 agctgtactc gggtgggtgg agctgcatca cttacttcga cgtcgagcaa cgccggttgg
     2521 atctggcggc cgagcttctc gatgtcagca ttccgctgat gctcgaacac gatctaccga
     2581 tttgccgtgt ctggcagatg ggttcgcgcg ccaggatgca cctcatggtg ggcgagtggg
     2641 acgacgcgac cgccgatgcc gatcgtgtcc tggacgcgcc gagcgcgccg ctggccagga
     2701 cgtggccgtt gctgataagg gcgttggtgg cattgcgccg gcgcgggcag ggggccgact
     2761 cgctcgatga ggcatggcaa ctcgcgtgcc gcttcggtga accgatccgg atgttgcccg
     2821 ccgcggcggc gatcgccgaa tcatgctggc tgacaggcgt acccgacgac cgattgatgc
     2881 agtgtcggaa actgctggcg gacgttccgg ccgaagggct ggagtggtcg aggggggagt
     2941 tgggggcctg gctgtgtcga ctcggcgaca ccgtcgacac ggcggacctg gcggcgcctt
     3001 atcggctcct tctgcagggc tctcacgagg cggcagctga agagttccac cgtctttcga
     3061 tgccttacga cgctgcgctg gcgctgctcg acagcgacga cctctcgctc acgggacgcg
     3121 cgctcgacat tctcgaccgg ctcggcgccg atgccgtcgc ggcgaaggtg cgccgcggcc
     3181 tgcgcgccag gggcgaatcc gccgtacctg cgcgcaggcg cacggcgacg ctggccaacc
     3241 cggccggtct gacggcccgt caggtcgagg tgctgcggct gctcgatgac gggctcacca
     3301 acgtggaact cgcggagcgg ctgtacctgt cggtgaagac cgtcgaccat catgtgtcgg
     3361 cgatcctcgc caagctggag gtgaacaagc gccgtgacgc ggttcgcaag gcgcggaagc
     3421 tgggaatcct ggccgattca gcgcccgtcg aagtaccgtt gtagccgccg ctgccgttgg
     3481 ccgaacagtg cgcggatgcc tgccgcgacg atcggcgtca gcaaccgcag cggccaccga
     3541 agctcgaagg tcaggcgatc gcgcacaacg gctttcgtgt cgcggtcgcg ttcgagcgtg
     3601 cgctcgtggc gccacagtcg catggacagc atcgtggact cctcgtggaa gccgcgaccg
     3661 ggccacaact cggcgatcag caggtcgtcg tagtcgaacg gcaggacgcc gaacagccgc
     3721 atccagcagc gacccaccgg gatgccgacc gggatggcgt cgacggtgag cgattccctt
     3781 cctcgtggca tcgacatcgt catccacggg cgcagttcgt cgttgatgcc ctccggcgtc
     3841 accacgcgcg cccacactcg ctccgttggt gcgcctacga tgctctgccg ctcgacgatc
     3901 atcgcctcga cgctatccgg gc
//
LOCUS       DASYLX010000797         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1470017,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000797 DASYLX010000000
VERSION     DASYLX010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470017"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..703)
                     /locus_tag="VGP27_18585"
     CDS             complement(392..703)
                     /locus_tag="VGP27_18585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV7853340.1"
                     /translation="MRGEVFQLHAPRGSRGHEQSGSRYAVVVQSDQLPLSTWLVAPTS
                     TSARAASFRPEIEIRGVNTRVLAEQAAAIDPGRLGKSVGFLSFDEMRRVDAALRIVLD
                     L"
     gene            complement(693..926)
                     /locus_tag="VGP27_18590"
     CDS             complement(693..926)
                     /locus_tag="VGP27_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853341.1"
                     /translation="MADTITFRPDEDTAKALEVLTKDGTAVSAAVRSALIDAARRKAG
                     AAIRAEAERLAEDESDRAEAIQVLRDMETLRAW"
     gene            1463..2359
                     /locus_tag="VGP27_18595"
     CDS             1463..2359
                     /locus_tag="VGP27_18595"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV7853342.1"
                     /translation="MHPGQLAVTPDMVRTLVAEQFPQWQHRRIAAVSSPGTVNAIFRI
                     GDQFVARFPLVPDDVAAVREQLEREAAAAEELTGKTPFATPQSVAIGEPGAGYPLPWS
                     INTWLPGEPATADSCGVSGSFAIDLGDFINAVRAIDTRGRTYDGNGRGGDLFSHDDWM
                     QQCLTNSEGLLDIATLRSIWAEMRDLPRGDTPDVMNHSDLIPPNTLVANGRLVAVLDV
                     GGLGAADPALDLVSAWHLLDTGRRQILRNHVGCDEAEWQRGRAWAFQQALGAVWYYVD
                     SSPTMSAGCRRTLERIVTDVRS"
     gene            2855..4504
                     /locus_tag="VGP27_18600"
     CDS             2855..4504
                     /locus_tag="VGP27_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7853343.1"
                     /translation="MPRSRPRRAATAACAALLFVAAGCSSDKPPQTTGAGDGKHVLLL
                     SIDGMHQSDLTGYVQAHPDSALAALVARGVSYTNAQTPVPSDSFPGLLAQVTGGNPRT
                     TGVYYDDSYANDLLEPGTTNCADAKPGGAVSFTEELDKDVTKIDGGQGLTNLPDGVLQ
                     MTATPTDVIDTAKLPVDPASCKPLMPGGYLKVNTVFAVAHDAGLRTAWSDKHPAYAIL
                     GGPGTSSIDDLFTPEVDSNPAGSKDADSWTADNALTRRYDTYKVDAVTNEIAGKDHSG
                     SRQVGVPSVFGMNFQAVSTAEKLSESGGQPGGYRPDGQTPGPVLASALAFVDTQIGRM
                     VSALNAAGMADTTTIIVSAKHGQSPMKPDTLTRIDDGQIIDGINAEWATTHPDVPELI
                     AHSVDDDAMIVWLADRSPAAAAFVADRLRATAGAGIDIAGKPKAFAASGTDRIYAGAE
                     AATFFGAIPGDPRVPDVYATTQPGTVYTGGTAKIAEHGGVTPADRHVPLIVAGPGVSA
                     RDDPSAVATAQIAPTILTRLHLDPNKLQAVVAENTAPLPGL"
     gene            4557..4775
                     /locus_tag="VGP27_18605"
     CDS             4557..4775
                     /locus_tag="VGP27_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853344.1"
                     /translation="MSGWVCCPRLVAALASTRHAGLPTAFTEPVIGPVRRVLKPVRLG
                     SVSVDLAFTAVFIAVVVLRTVVVPLIPF"
     gene            5504..5755
                     /locus_tag="VGP27_18610"
     CDS             5504..5755
                     /locus_tag="VGP27_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853345.1"
                     /translation="MGMKLRYITPLLAAGAAAVAIAAAPTAFATDQLSCNGSGSGTAC
                     LSPVNGPLKMSPSDVPHVAPGAMPDLTVRNFALALAMVT"
     gene            6157..>6428
                     /locus_tag="VGP27_18615"
     CDS             6157..>6428
                     /locus_tag="VGP27_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853346.1"
                     /translation="MYHTSRSCGAGEVATEHIEVNSLIEHDRAQLTFDPFHPMRASAR
                     PRPASAASVVMTLSLAIAVGVLLLVFYRPLESTAQGVAARSGISRLV"
BASE COUNT         1188 a         2109 c         1983 g         1148 t
ORIGIN      
        1 ctgtgcgctg tgcgccaata gccctgcgcc acagggcaat tcgtcactgt gacgtattgc
       61 cggcggcaca ggggagagcg aggatgggcg gtccaccggg gctgaccgcc gggggcatgc
      121 tcaaggcttc acctgacgcc cgaatcatct ataagtgatt gagctagatg gcaacctcca
      181 tgcacggagc ccgctggaga gtgagtaccc gaaatcactg tcgtgacgtg gtcagatcgg
      241 ccactcgtcg gggaggtgcc agcactttgc atctaatgca acgcgccgcc ttttgccgat
      301 aagccacatt atgtcaagtt acccccccat tagtggtggt gcgccaatcg ctttcattcg
      361 atccgattag gtggcggcgg cagtgcagcg ctcagagatc gaggacgatg cgcagtgccg
      421 cgtcaacgcg gcgcatctcg tcgaagctga ggaacccgac gctcttgccg agccggcccg
      481 ggtcgatcgc cgcggcctgc tcggcaagca cccgggtgtt cacgccgcgg atttcgatct
      541 cgggacgaaa gctggcggca cgagccgacg tagacgttgg cgcgaccagc caggtcgaca
      601 gaggcagttg gtctgactgg acgacgacgg cgtagcggga accggactgt tcgtgaccgc
      661 ggctcccccg cggggcatgc aactgaaaga cctcaccacg cacgcagcgt ctccatatcg
      721 cgcagcacct gtatggcctc agcgcgatcg gactcgtctt cggcaagcct ttccgcctcg
      781 gcgcgaatgg ctgcgccggc cttccgtcgt gcggcatcga tgagagcaga tcgaactgct
      841 gcggacacag ccgtgccatc tttggtcaaa acctccagcg ccttcgctgt gtcttcatcc
      901 ggccggaagg taatcgtgtc agccatagca acagtgtacg acgttttgta agacagttac
      961 cggatggtgt gaagcgacgt ttcgcagagc agcgacaccg ctggtaggcc tagccatcgc
     1021 gtcggagatc aaggcggtgt tgtgtgtccc ggacgctctc gagtgtcttg cccgactcac
     1081 cggggatgcc ggtggtgggc gcgacgctgt gcgcctcttc gatgccgcgg cagcccttcg
     1141 ccgacgcatg aaggcgccgc cggcatagag aactccaacg acatgggcta ctggcaggcg
     1201 ctcacaccgg acgagcttct cggccgagtc aacgccacca gacgtgcgat caatcgaacg
     1261 gccgccgcac tcggagctct cattgccggc ctcctcgtcg ggctgatcgg tgatcgcccg
     1321 acgctcatcg gcgtgatcat cgtttttcgc ggcgggcgcg ctccccacag cactatcacc
     1381 gctgcgagaa acacccaaca gagactgacc cggaaatcga gttgtcgata gcaacggggc
     1441 gtgcaacgct gcggccgtgc agatgcatcc cggccagtta gcggtcaccc ccgatatggt
     1501 gcggaccctg gtcgccgagc agtttccaca gtggcagcat cgacgaatcg cagctgtatc
     1561 cagtccaggc accgttaacg ccatcttccg gatcggcgac cagttcgtgg cacgcttccc
     1621 gctggtcccc gacgatgtgg cggccgtgcg ggagcaacta gagcgtgagg cagcagccgc
     1681 cgaagaattg accggcaaga caccgttcgc caccccgcaa tcggtcgcca tcggcgaacc
     1741 cggcgctggc tatcctctgc cgtggtcgat caacacctgg ctgcccggcg agcccgccac
     1801 agcagacagc tgcggcgtat ccggttcttt cgcaatagat ctgggcgatt tcatcaatgc
     1861 cgtgcgagcc atcgatacgc ggggacgaac ctacgacggc aacgggcgcg gcggcgacct
     1921 gttctcccac gacgactgga tgcagcaatg cttgaccaac agcgaagggc tgcttgacat
     1981 cgccactctt cgatccatct gggccgaaat gcgcgacctg cctcgcggcg atactcccga
     2041 cgtcatgaac cacagcgacc tcatcccgcc gaacacgttg gtcgccaacg ggcgccttgt
     2101 cgcggtactt gatgtcggag gcctcggtgc cgccgatccc gcgctcgacc tggtaagtgc
     2161 ctggcatctc ctcgataccg gacgccgcca aatactcagg aaccatgtcg gctgcgacga
     2221 agcggaatgg caacgaggac gggcctgggc ctttcagcaa gctctgggcg cggtctggta
     2281 ctacgtcgac agcagcccaa cgatgagcgc ggggtgccgg cgcaccctgg agcgcatcgt
     2341 caccgacgtc cgctcctgac accctcgaca gcaagacagc ggatctgatc atttccaggc
     2401 tgtcgaggtg ggcgaacgcc gcgtggcggt gtcattcaac gaaggatgtt cagcacgttt
     2461 tggagcggca ggcacgatgg ttgaacgtgg catctcatgc gttggttaaa cggctgccga
     2521 ggcggttgaa ttgttgctcg tgccgaatta cgccgcgatc ttggtgacgg ggatgtcggg
     2581 cgtggggaaa tcgacggcgt tggccgaact cacgcagcgc ggattcgcta ccgccgacaa
     2641 agacgagggg ccgtggtttc acgtcgtcga tgggagccgc tgtggcggag acgctgatcg
     2701 acgctcttct acatcggccc cgccagagcc aggcatcccc gcgaacctga aggatcgctg
     2761 aacgaccgcc cgcggacttc ttctgttcag tccacgttca tccccgccgg tcaggctgca
     2821 acggatacat tttccgtgac cagcagggag tcaagtgccc aggtcccgac ctcgacgggc
     2881 agctacggcg gcctgcgccg cgctattatt cgtcgccgcc ggctgttcga gcgacaagcc
     2941 ccctcagacc accggagccg gtgacggtaa gcacgttcta ctcctgtcaa tcgacggcat
     3001 gcatcaatcc gatctaacgg gatacgttca ggcgcatccg gattcggcac tcgctgcact
     3061 cgttgcacgc ggagtcagct acaccaacgc tcagacgccc gttccgtcgg actcgtttcc
     3121 cggtctcctc gcgcaggtca caggcgggaa cccgagaacg accggggtgt actacgacga
     3181 cagctacgcc aacgacctcc tcgagcccgg cacgacgaac tgtgccgatg cgaaaccggg
     3241 cggtgccgtc agtttcacag aggaactgga caaagacgtc accaagatcg acggcggtca
     3301 gggtttgacg aatctgcccg acggtgtcct gcaaatgacc gctacaccca ccgacgtgat
     3361 cgataccgcg aagctgcccg tcgatcccgc gtcctgcaag ccgctgatgc ccggtgggta
     3421 cctgaaggtg aacaccgtct tcgccgtcgc ccacgatgcc ggtcttcgga cggcttggtc
     3481 ggacaagcac cctgcctatg cgattctcgg tggcccgggc acctcgtcca tcgacgatct
     3541 gttcaccccg gaagtcgaca gcaatccggc gggaagcaag gacgccgaca gctggaccgc
     3601 cgacaacgcc ctcacccggc ggtacgacac ctacaaggtc gatgcagtca ccaatgagat
     3661 tgccggcaaa gatcattcag gcagcaggca ggtcggcgtc ccgtcggtgt tcggcatgaa
     3721 cttccaggcg gtctccaccg cggaaaagct gtcggagtcc ggaggtcaac cgggcggtta
     3781 ccgccccgac ggccagaccc ccgggccggt cctcgcctcg gcgctggcct ttgtcgatac
     3841 ccagatcggg cgcatggtgt ccgcgctcaa tgccgcgggg atggctgaca ccacgacgat
     3901 catcgtctcg gctaagcacg gtcagtcccc gatgaaaccc gacaccctca cccgcatcga
     3961 cgacggccag atcatcgacg gcatcaacgc cgaatgggcc actacccatc ccgacgttcc
     4021 ggagctgatc gcgcattcgg tcgacgacga cgcaatgatc gtgtggctgg ccgatcgatc
     4081 ccccgccgcg gcggcattcg tggccgaccg gttgcgcgcc acggccggcg cgggcatcga
     4141 catagctggg aagccaaagg cgttcgccgc ttctggtacc gatcggatct atgccggtgc
     4201 cgaggcggcg accttcttcg gcgcaatccc cggcgatccg cgggtgccgg acgtatacgc
     4261 gaccacccag cccggcacgg tctacaccgg cggaacagcc aagatcgccg aacacggcgg
     4321 cgttacccct gccgaccggc atgttccgct gatcgtggcg ggtccgggcg tcagtgctcg
     4381 cgacgaccca tccgcggtcg cgaccgcgca gatcgcgccg accatcctca cccggcttca
     4441 tctggatccc aacaagctcc aggccgtcgt cgccgagaac accgcaccgt tgccgggcct
     4501 gtaatgcgca ctcctacgtc tgtcagctga gctcataccg ctgacgataa ttcggggtgt
     4561 ctgggtgggt gtgttgtccc cggttggtgg ccgcactggc ctctaccagg cacgccggat
     4621 tacccacggc attcaccgag cccgtgatcg gcccggttcg ccgggtgctc aaacccgtgc
     4681 gactcgggtc cgtctccgtt gacttagcct tcaccgccgt ttttatcgcc gtggtcgtcc
     4741 tgcgaaccgt cgtggtgccg ttgattccgt tctgagtaca cagtcttgga gctgatcggg
     4801 gtggtgtgct cgccttcgcg gacgagacgg tcgacgtctg acctcgagca cggcacatca
     4861 ccgtcatcac cgtcaacgtc acggcacggt cctggcccat acgatgggcg cgcttacgag
     4921 ctcttctacc gtggttcgcg tcgtgtacac cgaaactgcg acttgcagtc gacgagtccc
     4981 agccgggcgg ggttcgcgct attgactcga tggccagcca gattcccccc caattcgttg
     5041 cgtcacgcca cagcgttttg atcgctcgat tccggtgatc gcgtgcggct ttgtcgattc
     5101 gcgaaacctg ctctggctta aggggactca ttggtcgcaa tcgtgagcga cagctacgac
     5161 cacaccgcga tgacgccgcg ccaggacgcc tgaaatcggc tcacgctatc tcagcgcgct
     5221 ttcagcatca cgcgggcaca ctttcggtat tgcgctgaca acggttcaaa gcaggctgag
     5281 gcgtggatat ttggcgacgt cacctcgatc actaggagga caatatgggt cgtggagatg
     5341 gcttcgcaac gaggtgcaca acgacaacat cactgcgctg atcggcgtgt tgatgggtgc
     5401 tgagcgggtt gtcggcgggc gtcaatggat tcacggcggt ggcggctaag gccagttctg
     5461 tacgggcaac tacacatcat cgagtacttc gaggagaacc accatgggca tgaaactgcg
     5521 atacatcacc ccactgctgg cggcgggcgc tgccgcggtc gcgatcgccg ccgcaccgac
     5581 ggcgttcgca acagatcagc tgtcctgcaa cgggagcggc tcgggaactg cctgcctgtc
     5641 gcccgtcaac ggtccgctca agatgtcccc gtctgatgtt ccgcacgtcg cccctggggc
     5701 gatgcctgat ctgacggtac gcaacttcgc gctggccctc gcaatggtga cgtgaacggc
     5761 ggcgccgcca cctggtcggg cgctcaggtg ccacgctgtt cagcagctcc atgctcgcga
     5821 accgcaagag ccgtttgcga cgacgcaact gcatgaagac gtagagcgtc accagcccga
     5881 caattgcgag caggaaggct gaagtgccac cgatgggtga aacctgaaag cgtcagtgat
     5941 cccgggatcg ccaaattcat gacgccgtcc accttcgcgc agtctgagca ccagcgccga
     6001 ccgacagcga ctcgcctcgg gtttggcgcg gatgattggc accgtacgaa ggcaggctta
     6061 gaaaccgctg aagacagggc ccgcgccgga agtaccacgc cgcacactgc gggtaggtct
     6121 aagccagtac ggcaacccag tacagggcac tcgagcttgt accacaccag ccgatcgtgt
     6181 ggcgccggcg aggtcgccac cgagcacatc gaagtcaata gcctcatcga gcacgaccgt
     6241 gcccagctga ccttcgaccc gttccacccg atgcgggcga gcgcgcggcc tcgtccggcg
     6301 tcggccgcct cggtggtgat gacgctcagt cttgccatcg cggttggtgt cctgctgctg
     6361 gtgttctacc gacctctgga gtccaccgcg cagggtgtgg ccgcgcgatc cgggatcagc
     6421 cgccttgt
//
LOCUS       DASYLX010000798         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3225297,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000798 DASYLX010000000
VERSION     DASYLX010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225297"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VGP27_18620"
     CDS             <1..140
                     /locus_tag="VGP27_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV7853347.1"
                     /translation="SKLAFARGYYNDSAATLNRLLRTMPWMFVAPLSGVSEREYYKIP
                     R"
     gene            143..1069
                     /gene="fmt"
                     /locus_tag="VGP27_18625"
     CDS             143..1069
                     /gene="fmt"
                     /locus_tag="VGP27_18625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV7853348.1"
                     /translation="MRIVFAGTPEPALPSLIRLIDSARHEVVAVLTRPDAASGRRGKP
                     APSPVAQLALEHGIPVLRPERPNSDEFVSQLAELAPEACAVVAYGAMLRDGLLAVPDH
                     GWINLHFSLLPAWRGAAPVQAAIAAGDTVSGATTFQIEPALDSGPVYGVVTETIRPTD
                     TAGDLLGRLSSSGAALLATTLDGIADGSLTPVPQPSEGVTMAPKVTVDDARVRWDLPA
                     HVVERRIRASTPNPGAWTVIGELRLKLGPVTVDEADPLAPGAIRVERNRVRVGTASQP
                     VLLGSVQPPGKKPMDAAAWARGARLDDSASAS"
     gene            1066..>1898
                     /locus_tag="VGP27_18630"
     CDS             1066..>1898
                     /locus_tag="VGP27_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV7853349.1"
                     /translation="MTKPRRPRRKPLDPARRAAFDVLRAVSERDAYANLVLPALLNER
                     GITGRDAAFATELAYGACRTRGLLDAVIAEAAGRPPDRIDPVLLDLLRLGAYQLLRTR
                     VEPHAAVSTTVEQAGIEFDSSRAGFVNAVLRTIAGREPQSWIDELAPPAGSDPVGHLS
                     FVHAHPRWIGQAFADALGARAGELKALLEADDARPLVHLAARPGVLTADELASVADGA
                     VGRYSPYAVYLAGGDPGRLDAVRDGAALVQDEGSQLVARALTLAPVDYDGGRWLDLCA
                     GP"
BASE COUNT          261 a          672 c          661 g          304 t
ORIGIN      
        1 agagcaagtt ggcgttcgcc cgcggttact acaacgactc ggccgccacg ctgaaccggc
       61 tactgcgcac catgccgtgg atgttcgtcg cgccgctgag cggcgtgtcc gaacgcgagt
      121 actacaagat cccgcggtag ctatgcggat cgtgttcgca ggcacacccg agccggccct
      181 gccgtcactg atccggctta tcgactccgc gcgccatgag gtggtcgcgg tgctcacacg
      241 tccggatgcc gcctccggtc ggcgcggcaa accggcgccc tcgcccgtcg cgcagctcgc
      301 gctcgagcac ggtatcccgg tgctgcggcc cgaacggccc aattccgacg aattcgtttc
      361 gcaactggcc gagttggcgc ccgaggcatg cgcggtcgtg gcgtatgggg cgatgctccg
      421 cgatggtctg ctggcggtgc ctgaccacgg ctggatcaac ttgcacttct ctctcctgcc
      481 tgcgtggcgt ggcgcggcac cggtgcaggc ggcgatcgcc gcaggcgaca ccgtcagcgg
      541 cgcgaccacg tttcagatcg aacctgcgct cgactcgggg cccgtgtacg gcgtcgtcac
      601 cgaaaccatc cgccccaccg atacagcggg tgatctgctt ggccggctgt cgagttcggg
      661 tgccgcgctg cttgcgacga cgctggacgg aatcgccgac gggtcgttga ctccggtacc
      721 gcagccgtcc gaaggtgtca ccatggcacc gaaagtgacg gtcgacgacg cccgcgtgcg
      781 ctgggacctg cctgcccatg tcgtcgagcg gcgcatccgc gcgtccactc cgaaccccgg
      841 tgcatggacc gtgatcggcg aactgcggct aaagctcggc cccgtgactg tcgacgaagc
      901 ggacccgttg gcgcccggcg cgattcgcgt ggagcgcaat cgcgtccggg tgggaacggc
      961 gtcgcaaccc gtgctgctcg gcagcgtcca gccgccgggc aagaagccga tggatgcggc
     1021 cgcatgggcg cgcggcgccc ggctcgacga ctccgcgtcg gcgtcatgac caaaccgcga
     1081 aggccgcgtc gcaagccgct cgacccggcc cgccgcgcgg cgttcgacgt cctgcgggcg
     1141 gtgtcggagc gcgacgccta cgccaacctc gtgctgcccg cgctgctgaa cgaacgcggt
     1201 atcacgggtc gtgacgccgc cttcgcgacc gaactggcct acggggcgtg ccggacgcgt
     1261 ggcttgctcg acgctgtcat agccgaagcc gccggacgcc cgccggaccg catcgatccg
     1321 gtgctgctgg acctgctccg gctgggggcc tatcaactgc tccgcactcg cgtcgagcca
     1381 cacgccgcgg tgtccaccac ggtggagcag gcggggatcg aattcgactc atcgcgagct
     1441 ggtttcgtca acgccgtgct gcgcacgatc gccgggcggg agccgcagtc ctggattgac
     1501 gaactcgccc cgcccgcggg gtcggacccg gtcggtcacc tctcgttcgt gcacgctcat
     1561 ccgcggtgga tcgggcaggc gttcgccgat gcgctcggcg ccagggccgg cgagctgaag
     1621 gccctgctgg aagccgacga cgcccgtccg ctggttcatc tcgccgcccg cccgggtgtg
     1681 ctgaccgcgg acgaacttgc atcggtcgcg gacggagcgg tcggccgtta ctcgccttat
     1741 gcggtctatc tcgccggcgg cgaccccggt cgactcgacg ctgtccgtga cggcgctgcg
     1801 ctggttcagg atgagggcag ccagctggtg gcaagggcgc tgacgctcgc gccggtcgac
     1861 tacgacggcg gccgttggct cgacctgtgt gcggggcc
//
LOCUS       DASYLX010000799         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1141312,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000799 DASYLX010000000
VERSION     DASYLX010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141312"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VGP27_18635"
     CDS             complement(<1..124)
                     /locus_tag="VGP27_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEV7853350.1"
                     /translation="MEAPELVALMQGRRVAVLTGAGMSTDSGIPDYRGPDSPPSN"
     gene            complement(261..800)
                     /locus_tag="VGP27_18640"
     CDS             complement(261..800)
                     /locus_tag="VGP27_18640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV7853351.1"
                     /translation="MSDTAVLVVDMLNSYRHKDAEKLVPSVAEVVEPIAGLINRTKKR
                     DDVDLIYVNDNYGDFTASRGDLVRQALEGERPDLVKPIVPDDDIRFLQKVRHSAFYAT
                     SLAYLLTQLGTKRLILTGQVTEQCILYTALDAYVRHFEISVPRDGAAHIDADLGKAAL
                     RMMAENMRAEIVSADECVR"
     gene            858..2912
                     /locus_tag="VGP27_18645"
     CDS             858..2912
                     /locus_tag="VGP27_18645"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEV7853352.1"
                     /translation="MDFYSAYGQGFVRVAACTLRTAIGEPATNADSVLRLARECHDEG
                     VGLAVFPELTLSGYSIEDILMQDALLGAVEDALLDIVAATVDLLPVLVVGAPLRYLHR
                     IYNTAVVIHRGQVLGVAPKSYLPTYREFYERRQMAPGDDVRGVIRIGRGSAVAEVPFG
                     PDLLFAATDLPGFVLHVEICEDMFVPIPPSAEAALAGATVLANLSGSPITIGRAEDRC
                     LLARSASSRCLAAYVYAAAGQGESTTDLAWDGQTMIYENGVQLAMSERFAKEPRRSVA
                     DVDLELLRAERLRMGTFDDNRAHHGLDADFFRRIEFELDPPAGDIGLRREVERFPFVP
                     SDPTRLEQDCYEAYNIQVAGLEQRLRALNYPKVVIGVSGGLDSTHALIVAARAMDREE
                     RPRSDILAFTLPGFATGDRTKGNAIELAEALGVTFEEIDIRATAELMLREMDHPFGRG
                     EKVYDVTFENVQAGLRTDYLFRVANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNV
                     NGGVPKTLIQHLIRWVVSSNQFDDRVNEVLTSVLDTEISPELVPQGEDEEIQSSEAKI
                     GPYVLQDFSLFQVLRFGFRPSKVAFLSWHAWRDPEHGDWPTGYPQDKRPSFSLKEIRH
                     WLQVFVQRYYSFAQFKRSALPNGPKVSHGGSLSPRGDWRAPSDMSAKTWLDEIERDVP
                     ED"
     gene            complement(3073..3678)
                     /locus_tag="VGP27_18650"
     CDS             complement(3073..3678)
                     /locus_tag="VGP27_18650"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV7853353.1"
                     /translation="MPAVGLLLFAVFGLLGFGWRSWLQHRHTGPPGFRGVAGCVGSVE
                     WFSGADFVVAMAAAFFAPVLQVFGVVSSLDLVYAPWIALTGIVLAVIGIVATVYAQLD
                     VGDSWRIGVDPSETTTLVRQGVFGLVRNPIFAAMFVFGLGIALVTPNVVAIAGFLLVL
                     TIEVQVRVVEEPYPATKHGDAYRDYLVSVGRFVPGVGLVSR"
     gene            complement(3693..>3852)
                     /locus_tag="VGP27_18655"
     CDS             complement(3693..>3852)
                     /locus_tag="VGP27_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014928165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HEV7853354.1"
                     /translation="LLLCSYLSAALLVGLVLNALVGWSLADPIAALAISALAVREGRN
                     AWRGDPCC"
BASE COUNT          685 a         1228 c         1266 g          673 t
ORIGIN      
        1 ggttgctcgg cggcgagtcc ggtccgcggt agtccggaat cccggagtcg gtggacatac
       61 ccgcgccggt gagcaccgct acccgccgtc cctgcatcag ggcgacgagt tcgggggctt
      121 ccaccccatc gagggtaggc ggtcggactt cctggtcgtg ttgtcgctga tgccgacagc
      181 agaattcggc tcagttggcc ggcgcgccca gccccggctt ttcgcgtcaa gccgaggtca
      241 ccagcagaag tccgcgtgga tcagcgcacg cactcgtccg ccgacacgat ttcggcgcgc
      301 atgttctccg ccatcatccg cagcgccgcc ttgccgaggt cggcgtcgat gtgtgcggcg
      361 ccgtcacgcg gcacgctaat ttcgaagtgg cgcacatagg cgtccagcgc ggtgtaaaga
      421 atgcactgct cggtgacctg cccggtgagg atcagccgct tggtcccgag ttgagtcagc
      481 aggtacgcca gtgaagtggc gtagaaggcg ctgtggcgga ccttctgcag gaaccggatg
      541 tcgtcatccg ggacaatcgg cttcaccaga tccggacgtt cgccttcgag cgcctggcgg
      601 acgaggtcgc cccggctggc ggtgaagtcg ccgtagttgt cgttgacgta gatgaggtcg
      661 acgtcgtcgc gtttcttcgt cctgttgatc aacccggcga tgggctcgac gacttcggcg
      721 acgctcggca ccagcttttc ggcgtccttg tggcggtagg agttgagcat gtcgacgacg
      781 agcactgcag tgtcactcac gactcactgg taccccgtag ccggtttatg acacgtcacg
      841 ggcgacaata gatcgtgatg gacttctatt ccgcttacgg ccagggtttc gtccgcgtcg
      901 cggcgtgcac cttgcgcacc gccatcggtg agcccgccac caacgccgat tcggtcttgc
      961 ggttggcgcg ggagtgccat gacgagggcg tcggcctcgc ggtttttccc gagctgacgc
     1021 tgtcgggcta ctccatcgag gacatcctga tgcaggatgc gctgctgggg gccgtcgagg
     1081 acgcgctgct cgacatcgtg gccgccacgg tggacctgct gccggtgctg gtggtcggcg
     1141 cgccgctgcg ctatctgcac cggatctaca acaccgcggt cgtcattcac cgcggacagg
     1201 tgctcggggt cgcgccgaag tcctatctac ccacatatcg ggagttctac gagcggcgtc
     1261 aaatggcgcc gggcgacgat gtgcgtggcg tcatccgcat tggccggggc agtgccgtcg
     1321 cggaggtgcc cttcggcccc gacctgctct tcgccgcaac cgatctcccc ggcttcgttc
     1381 tgcatgtcga gatctgcgag gacatgttcg tgccgatacc gccgagtgct gaggccgcgc
     1441 tcgctggtgc gacggtgttg gcgaacctgt cgggaagccc gatcacgatc ggccgggcgg
     1501 aggaccgctg cctgctggcc cgctcggcgt cgtcgcgttg cctggcggcc tatgtgtatg
     1561 ccgcggcagg acaaggggag tcgaccaccg acctggcgtg ggacggccag accatgatct
     1621 acgagaacgg cgtgcagctg gccatgtcgg agcgcttcgc gaaggagccg cggcgttcgg
     1681 tcgccgatgt cgatctcgag ctgctgcgcg ccgaacggct gcggatgggc accttcgacg
     1741 acaaccgggc acatcacggc ctcgacgcag acttcttccg acgcattgag ttcgagctcg
     1801 acccgcccgc aggcgacatc ggtctgcgcc gcgaggtcga gcggttcccg ttcgtcccat
     1861 ccgatcccac acggctggaa caggattgct acgaggccta caacatccag gtcgcaggat
     1921 tggagcagcg gctccgcgca ctgaactacc cgaaggtggt gatcggtgtc tcaggtggcc
     1981 tcgattccac gcacgcgctg atcgtcgccg cgcgcgcgat ggatcgggaa gagcggcccc
     2041 gcagcgacat tctcgcgttc acgttgcccg gcttcgcaac cggtgaccgc accaagggca
     2101 acgcgatcga gctcgccgag gcgctggggg tgacgttcga ggagatcgac atcagggcga
     2161 ccgccgagct gatgctgcgc gagatggacc acccgttcgg ccgtggcgag aaggtgtacg
     2221 acgtcacctt cgagaacgtg caggctggcc tgcgcaccga ctacctgttc agggtcgcca
     2281 accagcgcgg cggaatcgtg ctcggcaccg gcgatctgtc cgagttggcg ctcggatggt
     2341 cgacctacgg tgtgggtgac cagatgtcgc actacaacgt gaacggcggc gtgccgaaga
     2401 ccttgattca gcatctgatc cgctgggtgg tctcgtcgaa tcagttcgac gaccgggtca
     2461 acgaggtgct gacgtcggtg ctggacaccg agatcagccc cgagttggtg ccgcagggtg
     2521 aggatgagga gatccagagc agcgaggcga agatcggacc gtatgtgctg caggacttct
     2581 cattgttcca ggtcctacgc ttcggcttcc gtccgtccaa ggtggcgttc ctgtcctggc
     2641 atgcgtggcg cgatcctgaa cacggcgact ggccaaccgg atacccgcag gacaagcggc
     2701 cgtcgttctc gctgaaagag atccggcact ggctgcaggt cttcgtgcag cgctattatt
     2761 cgttcgccca gttcaagagg tccgcactgc cgaacggccc gaaggtgtcc cacggtgggt
     2821 cgctgtcgcc gcgcggtgac tggcgggcgc cgtcggacat gtcggccaag acatggctgg
     2881 acgaaatcga acgcgacgtt cccgaggatt agccgtctcg gcgccggcgc cactctcaac
     2941 gtgctgcggc gttcttggct gatgtgcagc gtggctctgg acagtccggc caggatcacc
     3001 gctcatatgt gccgatcaat ctgccgcgcg tgatgtgcag agctggagta tgtgtgaacg
     3061 cacgccggac ggtcaacgac tgactagacc gactccggga acgaacctgc caacggagac
     3121 cagatagtcg cggtaggcgt cgccgtgctt tgtcgccggg tagggctcct cgacgacacg
     3181 tacctggacc tcaatggtca gcaccagcag gaagccggcg atcgccacca cattcggcgt
     3241 taccagcgcg atacccagcc cgaagacgaa catggcggcg aagatcggat tgcgtacgag
     3301 cccgaaaacg ccttgacgca caagcgttgt cgtctcgctc ggatcgacgc cgatacgcca
     3361 ggagtcaccc acgtcgagct gcgcgtagac ggtcgcaacg atgccgatga ctgccagaac
     3421 aattccggtg agcgcaatcc acggcgcata gacaagatcc agcgacgaca ccacaccgaa
     3481 cacttgcagg acgggtgcga agaacgccgc cgccatggcg acaacgaaat ccgcaccaga
     3541 gaaccactcc accgagccga cgcatccagc caccccacga aagcccggcg gccccgtgtg
     3601 gcggtgttgc aaccaactgc gccaaccgaa tccaagcagg ccgaagacgg cgaacagcag
     3661 aagcccgaca gccggcatga tcgtttcttt ccctagcagc acgggtctcc ccgccaggcg
     3721 tttctgcctt cgcgaaccgc gagtgctgag atggccaacg cggcgatggg gtccgccaac
     3781 gaccatccaa caagggcgtt gagcacgagc ccaacgagca gggccgcgga cagatagctg
     3841 cacagcagca ac
//
LOCUS       DASYLX010000800         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3554843,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000800 DASYLX010000000
VERSION     DASYLX010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554843"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGP27_18660"
     CDS             complement(<1..256)
                     /locus_tag="VGP27_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7853355.1"
                     /translation="MQTVTHSDALSRFGCALSDPTRAEILLMLRDAPGYPSEFADKIG
                     VSRQILSNHLACLRGCGLVVAEPEGRRSRYRLADERIGHAL"
     gene            complement(376..1200)
                     /locus_tag="VGP27_18665"
     CDS             complement(376..1200)
                     /locus_tag="VGP27_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7853356.1"
                     /translation="MSTTETAPRPPDGDWLGTRFLSFRREGPFAVCTLDRPEARNAMT
                     PAMYFGIRYAVGLVEADPDLAGLLITGSGDVFAPGGDLGGGGDDNWLTFGAALGMDVT
                     PFEKLRQSAKPAVSAVNGLCQGGGLQIALCSDMAVVSERATFRVPELFRGIADTYYSQ
                     MLARLIGPVRTRDLMLTGRTLTAAEAEEWGMVSRVVPHAELMGTARDVLAQCCRTAPA
                     ARRVVKSSLDAYMGLFDRINMQASYSGEEAVEGFRAFKERRSPTWVHPDLRVDGRI"
     gene            complement(1212..>1703)
                     /locus_tag="VGP27_18670"
     CDS             complement(1212..>1703)
                     /locus_tag="VGP27_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV7853357.1"
                     /translation="ARAVIADSDVLLLQLEIPVTTAVEAARVARDAGTVVVVNASPAG
                     AKPHDLLALAQLADVVVVNEAEARDWHWPVTHLVITRGERGASYLGQDERFDVPAPAV
                     RPVDTTGAGDVFAGVLAAEWPAGHEQALRRACAAGALATLVAGAGDCAPFAEAIDGAA
                     ATG"
BASE COUNT          280 a          592 c          571 g          260 t
ORIGIN      
        1 cgagggcatg cccgatgcgt tcgtcggcca gccggtagcg gctgcgtctg ccctcgggct
       61 cggcgaccac caggccgcag ccacgcaggc aggccagatg gttggacagg atctgcctac
      121 tcacccctat cttgtcggcg aattcggagg ggtaaccggg cgcatcgcgc agcatcaaca
      181 gaatttcggc gcgagtggga tcggacaacg cgcatccgaa ccgcgacagc gcatcgctgt
      241 gagtcaccgt ctgcatgcgc aaacagtaca tcgagaaatg tattcagaaa aggctgaact
      301 actgcaatat ccgcgaacgt tccttaccag cccatatttc ggcggattct cgactggtaa
      361 ggaacgttcg cgcggtcaga tgcggccgtc cacccgcaga tcgggatgaa cccaggtcgg
      421 cgaccgtcgt tccttgaacg cgcggaagcc ttcgaccgcc tcttcgccgc tatagctggc
      481 ctgcatgttg atgcggtcga acagccccat gtaggcgtcg aggctggatt tcacgaccct
      541 cctggcggcg ggcgccgtcc ggcagcactg ggcgagcacg tcgcgggccg tgcccatcaa
      601 ttcggcgtgg ggcacgacgc gcgaaaccat gccccattcc tcggcctcgg ctgcggtcag
      661 cgtgcggcca gtgagcatca ggtcgcgggt acggaccggg ccgatcagcc gcgccagcat
      721 ctggctgtag tacgtgtcgg cgatgccgcg gaacagctcg ggcacccgga atgtcgcgcg
      781 ttcgctgacc accgccatat cgctgcacag tgcgatctgg aggccgccgc cctggcacag
      841 cccgttgacc gccgagaccg cgggcttggc cgactgccgc agcttctcga acggcgtgac
      901 gtccatcccg agtgcggcgc cgaacgtcag ccagttgtcg tcgccgcctc cgcccaagtc
      961 gccgccaggg gcgaagacgt cgccgctgcc ggtgatcagc aggcctgcga ggtctgggtc
     1021 cgcctcgaca aggccgacgg cgtacctgat gccgaagtac atcgcagggg tcatcgcatt
     1081 gcgcgcctcg ggccggtcga gggtgcaaac cgcgaacgga ccctcacgac ggaagctcag
     1141 aaaacgggtg cccaaccaat cgccgtcggg cgggcgaggg gcggtttcgg ttgtcgacat
     1201 gggagttacg cctacccggt cgcggccgcc ccgtcaatcg cctcggcaaa cggcgcgcag
     1261 tcgccggcgc ctgccaccag cgtggccagc gcgccggcgg cacacgcccg gcgcaacgcc
     1321 tgctcgtgac cggccggcca ctcggcggcc agcaccccgg cgaacacgtc gcccgccccc
     1381 gtggtgtcca ccggccggac cgcgggtgcg ggaacgtcga atcgctcgtc ctgacccagg
     1441 tagctggctc cgcgttcgcc gcgggtgatc accagatggg ttaccggcca atgccagtcg
     1501 cgagcctcag cctcgttgac gacgacgacg tcggcgagct gggcgagcgc caggaggtcg
     1561 tgcggcttcg cgcctgcggg cgatgcgttg acgaccacca ccgtgcccgc gtcgcgcgcc
     1621 actcgtgccg cctcgacggc ggtcgtgacg gggatctcca gctgaagcag cagcacgtcg
     1681 ctgtcggcga tgacggccct ggc
//
LOCUS       DASYLX010000801         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2237653,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000801 DASYLX010000000
VERSION     DASYLX010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237653"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1208..1561)
                     /locus_tag="VGP27_18675"
     CDS             complement(1208..1561)
                     /locus_tag="VGP27_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV7853358.1"
                     /translation="MTAIHQSRTGVSPGQRRPTPPTAVRAYASLLAITIVNPLTVVYF
                     AALVLGSQEGVAASTAEQTVFVAAVFIASASWQLALACGGALLGRFLTGPPGRLSTAL
                     ASSALIIALAVQQLV"
     gene            1700..>2302
                     /locus_tag="VGP27_18680"
     CDS             1700..>2302
                     /locus_tag="VGP27_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV7853359.1"
                     /translation="MLSYPSGMTVSSRALGVLSDALRAHRNQRRTRWRKLPPGRQALL
                     VVAHLRKGETYADLACGFTIGTSTVYRYIREALDLLAAMAPTLEQAIEVAQRKAFVIL
                     DGTLLRIDRVGMASGRDRAYYSGKHKAHGLNVQVIADPIGRLVWISPPLPGARHDMGA
                     AREHGIIDALTVHEIAAAADTAYQGAGPTVAVPQRRRRLDP"
BASE COUNT          386 a          729 c          776 g          411 t
ORIGIN      
        1 atgtagtcga gttcttcttc gctgcaatca acaggatggt caaacttgta tgaataggcg
       61 cctttgatgt tcgtccagag ctccggaccg ctagctatgg gcatgaatct cttggtccag
      121 tagtcgctgc cagcaagtat cttcattgga cgttgctccg gcggcaggct tcgcacccac
      181 tcttcgtggt ctcgctttgt tgtcaatagt cgctcaacgg gccatgcgtt tatctgcgaa
      241 tgaccatagt gtggtctttg ctacatagga gtatcaagtt gtcgtaatca tcgatcttcg
      301 tcgaatccgt gagaaattcc cgccgaggac ccctcgatga ttgcgcaata atgtgtgctt
      361 cctcgcccag gatggtatcg tagccggccg tcgtatcaaa caattcatct ctgcagatgg
      421 cgcacctgcc acccgaccga ccccaaagtc gtttccgtgt ctcgacgctg atcgacaacg
      481 ttccctccgc tccgcgaggg ttggagatcg ttgctccctc ggccgagtca tcgtccacgc
      541 cgcgacggcc gcgtcaaggg cggctgcgcc gtcgctgcgc gatgaactgc gctatgcctg
      601 cggcgccctt gactcacccg ccgctggcct ccgtgccacc gcggggtttg acctcaggtg
      661 gaacggctgc tcagagccag gctcacgccg cggcagggga ggcggctctg atatctcgtc
      721 gtgcagcgcg cctggtcgtt gccagctcga ccaaagcact gccgaggggg tatgcgatgc
      781 gcagaaggtg ccacatgacg atctggcgcg caggcgccag gtcgtcgctc ccgcgggtga
      841 gccgtaacca gttcaacgcg ggccgcaagt gccgctctca gcgatccgtc ccgggggaca
      901 tgttttcgtg gtgcgaaatg gaatgaacta gggtgggcac acgtcgctcg tgatgactcg
      961 cgcgcaactg ctggtgcagg tagaggctca gtgaggcgga acgccgccac cttttaatcc
     1021 gcgggttcag ggttcgatcc cttgggagcc ccacacgttg accagggctg acgttctcgg
     1081 tgacgcgtcg atggcgggcg gcgcgctgcg tgggatctgt gagtgccggc cggggttgga
     1141 atgccctggt ggcgggggtg agaccacgac gtcgcaagcg aggcagtgct ggcgaccgac
     1201 caccacttca tacgagctgc tgcacggcca acgcgatgat cagcgcgctg gatgctagcg
     1261 cggtcgacag acgaccgggt ggtccggtca ggaaccgacc gagtaatgcg ccgccgcacg
     1321 ccagggcgag ctgccagctg gccgacgcga tgaacacagc ggccacgaac acggtttgtt
     1381 cggcggtgga ggcagcaaca ccttcctgtg atccgaggac cagcgccgcg aagtagacga
     1441 cggtgagggg gttgacgatg gtgatcgcga ggaggctcgc gtaggcgcgg accgcggtgg
     1501 gcggcgtggg ccgccgctgt cctggcgaga caccggtgcg ggactggtgg atcgcggtca
     1561 ctgcaacccg caatgagcct ttgccaacct gacgttgggg ccggtcagcg ggtgtacggc
     1621 gtggcgtgac ctcgaacggg tgaagctcct ggtagacggg cgattgctga gatcaatccg
     1681 cctgaccagg agcttcaagg tgctgtccta cccgtccggg atgaccgtgt ccagccgcgc
     1741 gctcggcgtg ctctccgacg cgttgcgggc ccaccgcaac cagcgccgca cccggtggcg
     1801 caagctcccg cccggccggc aggcgctgct ggtcgtcgcg cacctgcgca agggcgagac
     1861 ctacgccgac ctggcctgcg gattcacgat cgggacctcg acggtctacc gctacatccg
     1921 cgaggccctc gacctgctgg cggcgatggc gccgaccctg gagcaggcga tcgaggtcgc
     1981 acagcggaag gcgttcgtga tcctcgacgg gacgctgctg cgcatcgacc gcgtcggcat
     2041 ggcgtcaggc cgagaccggg cctactacag cggcaaacac aaggcccacg ggctgaacgt
     2101 gcaggtcatt gccgatccga tcggcaggtt ggtgtggatc tccccgccgc tgcccggggc
     2161 gcggcacgac atgggtgccg cccgcgagca cgggatcatc gacgcgctga ccgtccatga
     2221 gatcgcggcg gccgccgaca ccgcctacca aggcgccggg ccgacggtcg cggtcccgca
     2281 acggcggcgg cgtctcgacc cg
//
LOCUS       DASYLX010000802         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_155028,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000802 DASYLX010000000
VERSION     DASYLX010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155028"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..798
                     /locus_tag="VGP27_18685"
     CDS             25..798
                     /locus_tag="VGP27_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7853360.1"
                     /translation="MVPRPIARGPWGSTISGTFIGGLLGRAIERAGGDPEFQPARLTV
                     DLLRPVALADPVRVDTTVERDGRRLRLMDASMRQNGTVVARASALFLRRTSQPDDPVW
                     ALPIDMPPPPAEAADTSGDFTMELWAYSANSDMAAPSMDLSEWRHGGQKYAWVREVKP
                     LVDGEPLTAFVRAAMAGDVTSSLTHSSASGLNFINADYTLTLSRLPDGEYIGLAALAH
                     YSHAGVATGTAAIFDHLGPIGSGVATGLANPGFRPPSRG"
     gene            complement(804..1283)
                     /locus_tag="VGP27_18690"
     CDS             complement(804..1283)
                     /locus_tag="VGP27_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7853361.1"
                     /translation="MDADKKPKQLNAPFVKTAMRYSSRAAAWLYRKSGGRIGGTWRVG
                     AGFKKPVPTLLLEHRGRKSGKLFASPLVYMTDGDNVVVVASMGGRDENPQWYHNLVAD
                     PDVHIEIGRDRRAVHAVLAGPEEKQQLWPKLVEAYADFDAYKAWTDRDIPVFILKPR"
     gene            complement(1285..1788)
                     /locus_tag="VGP27_18695"
     CDS             complement(1285..1788)
                     /locus_tag="VGP27_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7853362.1"
                     /translation="MGNNERAKIVMSDEEIAEFIERSRTATMATVLPDGRPHLVAMWY
                     GVIDGEVWFETKAKSQKAVNLRRDPTITVMIEDGQSYDTLRGVSIDGRAEIVDDPETN
                     LRVGISVWERYTGPYTDDMRPFVDQMMRNRICVRVVPGRIRSWDHRKLGMDPMPIGGS
                     TAQYLGS"
BASE COUNT          280 a          629 c          600 g          334 t
ORIGIN      
        1 ttcttcgccg tcgacgggca cgacctggtg ccaaggccga tcgcgcgagg tccatggggc
       61 tccacgatca gcggcacgtt catcggcggg ctgttggggc gggcgatcga acgcgccgga
      121 ggcgaccccg agtttcaacc cgcgcggttg accgtcgacc tgttgcggcc ggtcgcgctc
      181 gccgatccgg ttcgagtgga caccaccgtc gaacgcgacg gcaggcggct tcggttgatg
      241 gacgcgtcga tgcggcagaa cggcaccgtc gtcgccaggg ccagtgcact gttcctgcgg
      301 cgtaccagcc aaccggacga cccggtgtgg gcgttgccga tcgacatgcc gccgccgccg
      361 gccgaagccg cggatacgtc aggcgacttc accatggaac tgtgggccta tagcgccaat
      421 tcggatatgg ccgcccccag catggatctg tccgagtggc ggcacggcgg tcagaagtac
      481 gcgtgggtgc gcgaggtcaa gccgttggtc gacggcgaac ctctcacggc tttcgtccgt
      541 gccgcgatgg ccggtgacgt gacgagctct ctaacccatt ccagcgcaag cggattgaac
      601 ttcatcaatg ccgactacac gctgacgctg agccggctac cggacggcga gtacatcggt
      661 ctggccgcac tcgcccacta cagccatgct ggagtcgcca ccgggacggc cgcgatcttc
      721 gaccatctcg gccctatcgg cagcggggtg gcgacgggcc tcgcgaaccc gggctttcgg
      781 ccgccttcgc gcggctagcg gggctagcgc ggtttgagga tgaagaccgg gatgtcccgg
      841 tcggtccacg ccttgtatgc atcgaagtcg gcgtaggcct cgacgagctt cggccaaagt
      901 tgctgcttct cctcggggcc ggccagaacg gcgtgcaccg cgcgtcggtc gcgaccgatc
      961 tcgatgtgca cgtcggggtc ggcgaccagg ttgtggtacc actgcgggtt ctcgtcgcga
     1021 ccgcccatcg acgccacgac gacgacgttg tcgccatcgg tcatgtacac cagcggcgac
     1081 gcgaacagct tcccggactt gcggccccgg tgctcgagca acagcgtcgg cacgggcttc
     1141 ttgaaccccg cgcccacccg ccaggtgccg ccgatcctgc cgcccgactt gcggtacaac
     1201 caggcagccg cccggctcga atacctcatc gcggtcttga cgaacggcgc gttcagctgc
     1261 ttgggcttct tgtctgcgtc catgtcatga ccctaggtac tgtgcggtgc tgccgccgat
     1321 cggcatcggg tccatcccga gcttgcggtg atcccagctg cggatccggc cggggaccac
     1381 acgcacgcag atccggttgc gcatcatctg gtcgacgaaa gggcgcatgt cgtcggtgta
     1441 ggggccggtg tagcgctccc acacgcttat gcccacgcgc agattcgtct ccgggtcgtc
     1501 gacgatctcg gcgcggccat cgatcgagac cccgcgcaag gtgtcatagc tctgcccatc
     1561 ctcgatcatc accgtgatgg tcgggtcgcg gcgcaggttt accgccttct gcgacttggc
     1621 cttggtctcg aaccagacct cgccgtcgat gacgccgtac cacatcgcga ccaggtgcgg
     1681 tcgcccgtcc ggcagcaccg tggccatcgt ggccgtgcgg ctgcgctcga tgaattcagc
     1741 gatctcctcg tccgacatga cgatctttgc ccgctcgttg ttgcccacgt cgtcgatagt
     1801 ttcaggtccg cacccgggtc accgtcaccg ggatgccgtt cag
//
LOCUS       DASYLX010000803         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1141710,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000803 DASYLX010000000
VERSION     DASYLX010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141710"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGP27_18700"
     CDS             <1..373
                     /locus_tag="VGP27_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7853363.1"
                     /translation="NDPMVNIATLVANGTRVWVYCGNGKPGDVNGEIAGDNFNAKFLE
                     GFTLRTNKTFQEQYIAAGGKNGVFNFPSAGTHSWEYWGAQLQQMKPDIQRVLGATPQP
                     STPIATAESASTNTAAATTGG"
     gene            502..1380
                     /locus_tag="VGP27_18705"
     CDS             502..1380
                     /locus_tag="VGP27_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7853364.1"
                     /translation="MRGMIRAVLSVVLAAGVWSTVASVAKADGVEMLMVPSAAMGRDI
                     PVAFQGGGPHAVILLDAFNGGPDVSNWVGAGAFNTLAGKGISVAAPAGGAYSMYTNWE
                     QDGSKQWETFLADELPDWLAANKGLAPGGHGIVGAAQGGYGAMAMAAFHPNRYRYAGS
                     LSGFLTPSNTYMNGAITDGLARFGGVDIRNMWGLPQLGRWKWHDPDVHIQLLADNNTR
                     LWVFSPTTLGCADPAAMIGYCDQAQGSNRTFYQHYRGVGGHNGHFDFPQTGEHDWGTW
                     GPQLAAMSGELVTTIK"
     gene            1556..1915
                     /locus_tag="VGP27_18710"
     CDS             1556..1915
                     /locus_tag="VGP27_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7853365.1"
                     /translation="MSVQPPSTMQSRAEAKGGDPKADGTQPNGLTVTARQRLYLDALK
                     HAGVRPSTDLLALSIGSYVCQARAAKQSDQAVHDYVTPLVRGDVRNAQMNAVAPPPSE
                     VDAVTADYIRIATERLC"
     gene            1942..2949
                     /locus_tag="VGP27_18715"
     CDS             1942..2949
                     /locus_tag="VGP27_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7853366.1"
                     /translation="MATKNRRKRHRILALVAAGAMALVVALVVAVVWIMIRQPEKSQT
                     AVPPTAVPPTSAGPTTKPRPEFQDASCPDVQLMSIPGTWESSPQLDPFNPTQFPIALL
                     LNVTNPIRGQFGTDRVETYTVPYTAQFHNPLSADKQMSYNDSRAEGTQNAVKAITDMN
                     NRCPLTSYVLVGFSQGAVIAGDIASDIGNGRGPVDADLVLGVTLIADGRRQSSVGQDV
                     GPNPPGQGAEITLNEVPMLGEMGLTMTGPRPGGFGVLNDRTNEICATGDLICSAPEEA
                     FSIANLPRTMEILGGGAGQPVHALYATTQFWNLGGQPATQWTLDWTQKVLDNAPHPKH
                     G"
     gene            3244..>3702
                     /locus_tag="VGP27_18720"
     CDS             3244..>3702
                     /locus_tag="VGP27_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7853367.1"
                     /translation="MGFHNPFIKDGLIRFPDSGSLVLHVERWTKVRGDKVAYRFLDFS
                     TERDGVGRDLNWADFGRRNRAVGARLQQVTEPGDRVAILCPQNLEYLVAFFGTLYSGR
                     IAVPLFDPSEPGHVGRLHAVIDDCHPSAILTTTESAEGVRKFFRSRPAKER"
BASE COUNT          661 a         1207 c         1241 g          593 t
ORIGIN      
        1 caacgacccg atggtcaaca tcgccacgct ggtcgccaat ggcacccgcg tctgggtgta
       61 ctgcggtaac ggcaagcccg gtgacgtcaa cggtgagatc gcaggcgaca acttcaacgc
      121 gaagttcctc gagggcttca ccctgcggac gaacaagacg ttccaggagc agtacatcgc
      181 cgcgggcggc aagaacggtg tgttcaactt cccgtccgcc ggtactcaca gctgggagta
      241 ctggggcgca cagctgcagc agatgaagcc cgacatccag cgcgtgctgg gtgcgacgcc
      301 gcagccgtcc accccgattg ccactgctga gtcggcctcg accaacaccg cggcggcgac
      361 gacgggcggc tagccacccg atcacggata tgaggtctgg cggcggcgat ccttcggggt
      421 cgccgccgcc agccgtttcc ggcaacccag gtgatgtggt tcactgcatt gcgcggggac
      481 agcgaagata agggtgggta catgcgcggg atgattcgag cggtcttgtc ggtggtgctg
      541 gcggcgggcg tgtggtcgac cgtagcgtcg gtcgccaagg ccgatggcgt cgagatgctg
      601 atggtgccgt ccgccgctat ggggcgagac atccccgtcg ccttccaggg cggcggcccg
      661 catgcggtga ttctgctcga cgcgttcaac ggcggccccg atgtgagcaa ctgggttggc
      721 gcgggcgcct tcaacacgct ggcgggcaag ggcatctcgg tggccgcccc ggcgggcggc
      781 gcctacagca tgtacaccaa ctgggagcag gacggcagca agcagtggga gacgttcctg
      841 gccgatgagc tgcccgattg gctggccgcc aacaagggcc tggcgccggg cgggcacggc
      901 atcgtcggcg ccgcccaggg cggctacggc gcgatggcga tggcagcctt ccatccgaac
      961 cgctaccgct acgccggctc gctttcgggt tttctgacgc cgtcgaacac ctacatgaac
     1021 ggtgcgatca ccgacgggct ggcgcggttc ggtggtgtcg acatccgcaa tatgtgggga
     1081 ctgccacagc tgggccggtg gaagtggcac gacccggacg tccacattca gctgctggcc
     1141 gacaacaaca cccggctgtg ggtgttcagc cccaccacgc tcgggtgcgc tgatcccgcg
     1201 gccatgatcg gatactgcga ccaggcccag ggcagcaacc gcacgttcta tcagcactac
     1261 cgtggcgtcg gcgggcacaa cgggcacttc gacttcccgc agaccggcga gcatgactgg
     1321 ggcacttggg gcccgcagct ggccgcgatg tccggcgaac tcgtcaccac gatcaagtga
     1381 cctgcggacg cgaggagcac aggggcttcc gtagcgccgg taccttggag gagtgcagca
     1441 cacggttcgg cgttggccga ggtggtcgac cagcgtcgcc gtagcgacgt ctctcgccgc
     1501 cccccttact gccgtgctga ccggttgcac ctccggtgac ggtatgttgc cgaccatgtc
     1561 ggtgcagccc ccgtcgacga tgcagtcccg cgccgaggcg aagggtggcg acccgaaggc
     1621 cgacggcacc cagccgaacg gactgacggt gaccgcccgg cagcggctat atctggatgc
     1681 gttgaagcat gcgggcgtgc ggccgtcgac cgacctgctg gcattgagca tcgggtccta
     1741 cgtgtgccag gcgcgcgccg ccaagcaatc ggatcaggcg gtgcacgact acgtgacgcc
     1801 gttggtgcgc ggtgatgtgc gcaacgctca gatgaacgct gtggcccccc cgccctctga
     1861 ggtcgacgcg gtgaccgccg actacattcg gatcgctacc gaacgactct gctgaacaga
     1921 ctctcggcga aaaagggatc aatggccacc aagaatcggc ggaaacgcca ccgaatactc
     1981 gcgctcgtcg cagcgggagc gatggcgctc gtggtggcgc tcgtcgtcgc ggtggtgtgg
     2041 atcatgatcc gtcagcccga gaagtcgcag accgccgtac cgccgaccgc ggtgccgccg
     2101 acgagcgcag ggccgacgac gaagccgcgc cccgagtttc aggacgccag ttgccccgat
     2161 gtgcagttga tgtcgatccc cggcacctgg gagtcgtcgc cgcaactcga cccgttcaac
     2221 ccgactcagt tcccgattgc gttgctgctg aacgtgacca acccgatccg cgggcaattc
     2281 ggtaccgacc gggtggagac gtacaccgtt ccctacacgg cgcagttcca caatccgctg
     2341 tccgccgaca agcagatgtc ctacaacgac agccgcgccg agggcaccca gaacgcggtc
     2401 aaggcgatca cggacatgaa caaccgctgc ccgctgacca gttatgtgct cgtcggcttc
     2461 tcccagggtg cggtgatcgc cggtgacatc gcgagcgaca tcggtaatgg gcgcggtccc
     2521 gtcgacgcgg atctggtgct cggcgtgacc ttgatcgccg acggccgcag gcagtcgagt
     2581 gtcgggcagg acgtcggccc gaaccctccc ggccagggcg ccgagatcac gctgaacgaa
     2641 gtgccgatgc tcggggagat ggggctgacg atgacgggcc cgcgcccggg cggcttcggt
     2701 gtgctcaacg accgcaccaa cgagatctgt gcgaccggcg acctgatctg ctcggcgccg
     2761 gaggaggcgt tctcgatcgc gaacctgccg aggacgatgg agatcctcgg cggcggcgcg
     2821 ggccaacccg tgcatgccct gtacgccacg acgcagttct ggaatctggg cggtcagccg
     2881 gccacccagt ggaccctgga ttggacgcag aaggtccttg ataacgcgcc gcatccaaaa
     2941 catggctgac acgtgaccgc atagatttgg cctgcgcgat gcggtcgcct aacattaaga
     3001 aataactaag accaatgact tacagccgaa tggaaaacac tggccacagg ccatagcggc
     3061 gcgtgcttgc gggctgccga cctgtggctc gacagcggac accaatgcct cggtagcatc
     3121 ttggaggttt gggttcgcag gaacaacacg gacaaccaag ttcctcacga cgcccgccgc
     3181 ggcggcccga cggtgctcgt gagaacgagc gccgccatga gtgtgctttg acaggagagt
     3241 gcgatggggt tccataaccc gttcatcaag gacggactga tcaggtttcc ggacagcggc
     3301 agcctggttc ttcacgtcga acggtggacc aaggtgcgtg gagacaaggt cgcctaccga
     3361 ttcctggact tctcgaccga gcgcgacggc gtcgggcgag atctgaactg ggccgacttc
     3421 ggcaggcgta accgtgccgt cggtgcccgc cttcagcagg tgaccgagcc cggcgaccgg
     3481 gtcgccatcc tgtgcccaca gaatctcgag tacctcgtcg ccttcttcgg cactctgtac
     3541 tccgggcgca tcgcggtgcc gctgttcgat ccgagcgagc cgggccatgt cggccggctg
     3601 cacgccgtca tcgacgactg ccacccgtcg gccatcctga ccacgaccga gtcggccgag
     3661 ggtgtccgca agttcttccg cagcaggccc gccaaggagc gg
//
LOCUS       DASYLX010000804        13243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1470532,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000804 DASYLX010000000
VERSION     DASYLX010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470532"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VGP27_18725"
     CDS             complement(<1..1398)
                     /locus_tag="VGP27_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7853368.1"
                     /translation="MAGPRTEAEDLAPNGMPASIPELLAIRRGAPDGEFLITDHERLT
                     FAEADTASLELAEALLVFGVGKGTRVGILFPNCAQWLICWLAAARIGALTVPLSTFAP
                     GAELVRLLRHTDTQVLLMGRSIAGRDLVGRVADALPGLAEGDSAISLEAVPFLRRVHV
                     WPGCDLPWSMPWPGSTGSSAPLTVSAEQEVRPSDDLVLVTTSGTTALPKSVAHTHGSL
                     VRHAYILARHRGVTSADRIYSPMPFFWVGGLTMVVLQALSTGAAILAQDVFDAGSTLA
                     LLERERATFISCWAQASQAMVDHPDFSKRDLTSVRGGTMLEALPPERRPSEPGLTPNM
                     LGMTETGGPHTMVEVPDTPLPTERRGSFGIPLPGVVAHRIVDTAGLEAAPGQEGEIQV
                     RGQILMSGIYKQERHDVFTTDGWYSTGDRGWFDEAGHLHFTGRASALIKTAGSNVSPA
                     EVESVLDAVPGVLHSF"
     gene            complement(1398..2642)
                     /locus_tag="VGP27_18730"
     CDS             complement(1398..2642)
                     /locus_tag="VGP27_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853369.1"
                     /translation="MSTEQGTASAVQADGDGSRREFNPYSDSFFDDPYDTYRWMRDEA
                     PVYYSERWDFYALTRNADVVAAHRDWETFSSSYGVTLDALSMRRQFSDLNMLILLDPP
                     AHERLRKLVRQVFTKAAIANLEPLVTEVVTSHVRALDGKDDFDLVADFAALFPVEIIS
                     SMLGVPEGERQQIRHWTDAFLHREKNNPFATESGVAASMAMGEYFLELAREKRRNPDE
                     LIISRLVTTTYEDEAGVTHRLTDDDIATFSVLLAAAGSETVTKLIGNGVMAFHHNPGQ
                     WEQVLADPGVIPGAIEEMLRLNPPSQYQGRFTTRDVTLDGGTIPAGSPTLLVTGAATR
                     DPRAYDHPDEFDIKRGGATTLAFGYGVHSCLGSWLARLETRVALQQLRERWPRFEVDT
                     DGLQRVTMSNVAGYSHIPVHIG"
     gene            complement(2644..3828)
                     /locus_tag="VGP27_18735"
     CDS             complement(2644..3828)
                     /locus_tag="VGP27_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853370.1"
                     /translation="MTSRLGFPVFDGDNHMYETRDALTKFLPPDYHGAVRYVEVDGRT
                     KIATLGQISEYIPNPTFDKVAAPGAQEDYFHNGNPEGKSHREILGKAIESTPAMREPA
                     PRLALLDEQGIDKSMMYPTLASLVEERFREHPEATHTIIHALNQWLHETWSFDYKGRI
                     FTTPIITLPIVEKAIDELNWVVEHGAQAILIRPAPVPGLHGLRSFALPEFDPFWKRVV
                     ETDVLVIMHASDSGYSRYANDWEGAGEFRPFQPSPFRSYYSLARNPAEDAVAAMACHG
                     LLTRFPELRIGMVENGTTWLPRLLQNLSDTHKKMPQLYGEDPVAAISRCVYFNPFWEE
                     NVAELAKLIPVDHLFFGSDYPHPEGLADPLSYPERHPELSDDEIAMVMGGTLSRMMRA
                     DA"
     gene            4055..5083
                     /locus_tag="VGP27_18740"
     CDS             4055..5083
                     /locus_tag="VGP27_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7853371.1"
                     /translation="MDLGLTPEQRAVCDAFAALFKKQATPERVRRAESTGFDELLWRH
                     YADIGALGIGVPSDCGGGDGGLLELALVAEEAGGRLAPIPVAETSAAARTLGRLGEKE
                     LLEPVLTGSVIVSLATRAGPVSQQMLVDGAIADAVLVLDGDKLVYVARPVGLAKVARR
                     NLGALPLGKWDDAPGATSVLAEGSEARMAFDTAVDEVRVLRAAALVGLATEAVGIGSR
                     YAIVREAFGQPIGMYQAVAHPFADAIAARDGAQLLVRKACWALDSGHEDAGALAAMAF
                     VFAAETAHHATAHSLHVHGGYGFMEEYDIQLYYRRAKAWAAAFADPRRELLTVADRRF
                     GPATTGGR"
     gene            5088..6254
                     /locus_tag="VGP27_18745"
     CDS             5088..6254
                     /locus_tag="VGP27_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853372.1"
                     /translation="MDFTEPQNWKRIRREAEAFVAAHVTREVIDRERRTGDGVDRALT
                     RKLGERGWIASGWPVAEGGAGLDPFEAAALWDALRHGGVPTIGPGTTMLPANAIRATG
                     SEELKARILPGVAAGEILICLGYTEPAGGSDVFACATRSQRDGDEWIVNGQKIFTTFA
                     HLADYCFLMTRSQPGSAGTRGLTMLLVPLDSPGIEIQAVHTMGQERTNIVFYNDVRVA
                     DANRVGEAHEGFAVMRAALEAEQNVAPASRTGWVARDALEFARTHRGPDGAELIDDVL
                     VRERLARMAMEAEVADLLGLRVAFLDAEGERPGPVSALFGPESYVRASAAAIDIAGSA
                     GMLDWTDPQSAVDGSLDAHYRSAVATTIYGGSSEVLRSLIVENRLGFPRSRPRR"
     gene            complement(6283..7077)
                     /locus_tag="VGP27_18750"
     CDS             complement(6283..7077)
                     /locus_tag="VGP27_18750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853373.1"
                     /translation="MDLGFADATAVIAGGTRGIGLATAACVAEQGARVAIIGRDDNRL
                     AAAGTALAAAGSPEVLTLKADLGSPSDVEDAFATVGARWGALNVLVNAAGPAGAGRFE
                     DLDDMLWTAAFDSGVVSAVRTVRAALPLLRAAPWARVVNVTAMSVQHQSAGLVSYTAA
                     KSALLSVTKNLARSLAGDAILVNAVAPGPILTDWARPMLAAAGVEPTDPVGAFALLGR
                     DHGMSVDLGRFGLPQEVAAVIAFCASRANTYMTGAHLNVDGGSDFL"
     gene            complement(7088..8026)
                     /locus_tag="VGP27_18755"
     CDS             complement(7088..8026)
                     /locus_tag="VGP27_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853374.1"
                     /translation="MTVNSANWIDPEIAECLRGFPDFDFSAENLAAMRAGSTFEPQSA
                     SDIERTELITERGDVALSVLRPIELGDTPPAVYWMHGGGMVIGNRHMDDTRLMEWCRW
                     LGCVCVSVEYRLAPEAPYPAPLDDCDEGLRFIVDHAVDLQIDPRRVGIAGRSAGGALA
                     ASLAMRWRDGGQVPLLFQYLEYPMLDDRGLTVSSQLERLPVWSREANAFGWSAYLGDR
                     YDTDGVPADAASARATELCGLPPTFVGVGAVDCLRDESIEFAARLCRAGVPTELHVYA
                     GAVHGFDMFASTAVARTAARDSADWLARQFDRRVVA"
     gene            complement(8023..8862)
                     /locus_tag="VGP27_18760"
     CDS             complement(8023..8862)
                     /locus_tag="VGP27_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853375.1"
                     /translation="MAVDNILVITGGAGGMGLACARALADRGRLLLVDLREDLLDQAR
                     KALGAQGAVVDTLSCDVTSATDVAAVADRVSGLGRLRCLVHTAGVSPEMADAATVLDV
                     DLAGSVRITDALFPLVNPGSSAILIGSIAGYSDVPAAVEPFLDDPLADGFLAAIEQAL
                     GQPLDSATAYVLAKRGVIRLVERLATPWGKTGGRAVSISPGLIDTPMGRLELDRQPIV
                     PVMVEVTPVKRPDRPLPGRPGDIAAAVAFLESDGAAFISGCDIRVDGGLVGAGRHLMG
                     VGG"
     gene            complement(8897..10090)
                     /locus_tag="VGP27_18765"
     CDS             complement(8897..10090)
                     /locus_tag="VGP27_18765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7853376.1"
                     /translation="MNNAVVVDVVRLASGKGKPGGALSGCHPVDLLRQVLVEILTRND
                     LDPGCIDDVIAGCVEQVGQQSFNIARSAVLAAGFPESVPATTVDRQCGSSQQAVHFAA
                     QGVMAGAYDAVIACGVESMSRVPMGTASSGADTFGPSVAARYPQGLVKQGVAAELVAE
                     RWKLDQEALDSFAVTSHQRAAAAAADGCFANEIVAMPVVDATGRPGTHAVDETIRADS
                     TTQALSGLKPSFASTEAAERFPVLEWRITPGNSSPLTDGASAALIMSERMALASGLRP
                     RARFHAFSVAGDDPLYMLTAPIPATQRALHRGGLTLDDIDAYEVNEAFACVPLAWAQE
                     LGADPERLNPWGGAIALGHALGSSGTRLLATLVNHLEATGGRYGLQTMCEGGGMANAT
                     IIQRL"
     gene            10234..10428
                     /locus_tag="VGP27_18770"
     CDS             10234..10428
                     /locus_tag="VGP27_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7853377.1"
                     /translation="MKVTVDPDLCEANALCVAEAPEVFDLSDDEVVHILLAKPPTGLE
                     PSVIEAVSACPKQALRIVTD"
     gene            10561..11688
                     /locus_tag="VGP27_18775"
     CDS             10561..11688
                     /locus_tag="VGP27_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853378.1"
                     /translation="MLPDDAKIISVDDHVIEHPRVWLDRVPAKYAEVAPRIVKLPDGN
                     DTWLYEGKQSGNFALNAVAGKHPRDFGMDPRSYDDMLPGCHDIEDRIRDMDIEGVWAQ
                     LCFPNFGGFAGSTFHTATDKDFAKICISAYNDFILDEWCSYAPERQIPLMMVPFWDIE
                     ASVAEIERTAAKGAKSISFIEAPHKIGLPSYHTDHWDPIFHICEEAGLPLSMHFGSGG
                     MPQGLAPDGDLFIGIALFGLNSMMATVDLLMSDVFYKFPNLKVALSEGGIGWMPYLLE
                     RIDYSWGRHKYWCDINPDKLPSDLFREHIYGCFISDQSGIEQRHRIGIDNIMFESDYP
                     HSDSNWPHTRKLLAEHLVDVPDEEARKIVELNARTLYNFWA"
     gene            11695..12861
                     /locus_tag="VGP27_18780"
     CDS             11695..12861
                     /locus_tag="VGP27_18780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7853379.1"
                     /translation="MPDAFILSAARTPIGRARKGSLVSVDAYQLAEVAVGAAVQRSEI
                     AIAEFDDLILAESLQGGGVIARNIAVRLGMTSVPGVAINRHCASGATAVQLAAATIMA
                     GMADVIVAGGTESASTMPRLTKMAPGAQEPTPWSPQSHPDAPGVPAFDMSVTIGENTA
                     RLHHVTREQADAWSARSHQRALNAIAKGYFDDEIVAVPVGHGATTVMFGTDEHPRDTT
                     PEALAGLKVLHPEMPGAVVTAGNSAGINDAAAALVIGSADFAASHGLAPLARIRGWTS
                     VGVEVKHTGMAPVTAIPLALKRSGLTLDDVDLVEINEAFATMAVACTRDLGLDESIVN
                     VNGSGISLGHPIAATGARMVVSIIHELARRDSRIGVVAMCAGGGMGSAMVVERI"
     gene            complement(12877..>13243)
                     /locus_tag="VGP27_18785"
     CDS             complement(12877..>13243)
                     /locus_tag="VGP27_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853380.1"
                     /translation="DLEGRYLRTFGEAFLNSPSDFARWGDLLVVAEIYERLAVLDSED
                     HLVGYIGANPDPEAAQGWPTRPGWPNDLADDGRAEIPDLPQHDRFNSPHAVATDADGN
                     LYVTEWLLGGRYTKLAVRR"
BASE COUNT         2308 a         4346 c         4387 g         2202 t
ORIGIN      
        1 gaaactgtgc agcaccccgg gcaccgcatc gaggaccgac tcgacctcgg ccggtgagac
       61 attggaccct gcggttttga tgagcgcact ggcgcggccg gtgaagtgca gatgcccggc
      121 ttcgtcgaac cacccgcggt ccccggtcga ataccagccg tcggtggtga acacgtcgtg
      181 gcgttcctgt ttgtagatgc cgctcatcag aatctggccg cgtacctgga tctcgccttc
      241 ctgacccggc gctgcctcca ggcctgccgt gtcgacgatc cggtgcgcaa ccacgcccgg
      301 tagcgggatc ccgaaggaac cgcgccgttc ggtcggcagc ggcgtgtcgg gcacttccac
      361 catggtgtgg gggccgccgg tctcggtcat gccaagcata ttgggcgtca ggcccggctc
      421 gctcgggcgc cgctcgggcg gcaacgcctc cagcatcgtg ccgccccgga cgctggttag
      481 gtcgcgcttg gagaagtcgg ggtgatcgac catggcctga ctggcctgtg cccagcagga
      541 gatgaaggtg gcccgctccc gttcgagcaa cgccagcgtg gagccggcgt cgaacacgtc
      601 ctgagccagg atcgccgcgc ccgtcgacag cgcctgcagc accaccatgg tcaggccgcc
      661 tacccagaag aacggcatcg gggagtaaat ccggtcggcc gaggtgacac cgcgatgtcg
      721 cgcaaggatg taggcatggc gcaccaggct gccgtgggta tgcgcaacgg atttcggcag
      781 cgccgtggtt ccggacgtgg tgacgaggac gagatcgtcg gagggccgga cctcctgttc
      841 agccgagacc gtcagggggg cggacgaacc cgtgctgccg ggccacggca tggaccaggg
      901 caggtcgcag ccgggccaca cgtgtacacg gcgcaggaag gggacggcct caagcgagat
      961 cgcgctgtcg ccctcggcca gaccgggcag cgcgtcggcg actcggccga cgaggtcgcg
     1021 gccagcgatc gaacggccca tcagcagcac ctgggtgtcg gtgtggcgca gaagccggac
     1081 gagttctgct ccgggcgcga atgtcgacag cgggacagtc agcgcgccga tacgggcggc
     1141 cgccagccag cagatcagcc actgggcgca gttgggaaac aagatgccga ccctggttcc
     1201 cttgccgacg ccaaatacca gcagggcctc ggccagctcg agcgacgcag tgtcggcttc
     1261 ggcgaaggtc agccgctcat ggtcggtgat gaggaactca ccgtcgggtg ctccccgcct
     1321 gatagccagg agctcgggga tgctggcggg catgccgttg ggcgccagat cctctgcctc
     1381 cgtgcggggc ccggccacta gccgatgtga acggggatgt gcgagtagcc ggcgacgttc
     1441 gacatcgtca cccgctgcag accgtcggtg tcgacttcga agcgcggcca gcgttcgcgc
     1501 aactgctgca aagcgactcg agtttcgagg cgcgcgagcc aggaacccag gcagctgtgt
     1561 acgccgtatc cgaaggccag cgtggtggct ccgcctcgct tgatgtcgaa ttcgtccgga
     1621 tggtcgtagg cgcgcgggtc acgcgtcgcg gcaccggtca ccagaagggt cggcgagccg
     1681 gccgggatcg ttccgccgtc gagcgtgacg tcgcgggtgg tgaacctgcc ctggtactgc
     1741 gacggggggt tgagtcgcag catctcctcg atcgcacccg ggatcacccc ggggtcggcg
     1801 agcacctgct cccactgccc gggattgtgg tgaaacgcca tgaccccatt gcctatcagc
     1861 ttggtgaccg tttcactgcc cgccgcggcg agcagcaccg aaaacgtagc gatgtcgtcg
     1921 tcggtgaggc ggtgcgtcac ccccgcctca tcttcatagg tcgtggtcac cagccggctg
     1981 atgatcagct cgtcggggtt gcggcgcttc tcccgcgcca actccaagaa gtactcgccc
     2041 atcgccatcg acgccgcgac gccgctctcg gtggcgaacg ggttgttctt ctcgcggtgc
     2101 aggaacgcat cggtccagtg ccggatctgc tggcgttcgc cctcgggcac gccgagcatc
     2161 gacgagatga tctctaccgg gaacagcgcc gcgaagtcgg ccaccagatc gaagtcgtcc
     2221 ttgccgtcca gggcgcgaac atgggatgtc acgacttcgg tcaccagtgg ctcgaggttg
     2281 gcgatcgccg ccttggtgaa cacctggcgc accagcttgc gcagccgctc gtgcgcgggg
     2341 ggatccagca ggatcaacat attgaggtcg ctgaactgtc ggcgcatcga caacgcatca
     2401 agggtcacac cgtaagaact gctgaacgtc tcccagtcgc gatgcgcggc aaccacatcc
     2461 gcgttgcgtg tcagggcgta gaaatcccat cgctcgctgt aatacaccgg cgcctcatcg
     2521 cgcatccagc ggtaggtgtc atacggatcg tcgaaaaagc tgtcggagta cgggttgaac
     2581 tcccgtcggg atccgtcacc gtctgcttgg acggccgatg ccgtgccctg ttcggtcgac
     2641 atctcaggcg tcggcccgca tcatgcgcga aagcgtgccg cccatcacca ttgcgatttc
     2701 gtcgtcgctc aactcgggat ggcgttcggg gtagctgagt ggatcggcca ggccttcggg
     2761 atgcgggtag tccgagccga agaacaggtg atcgaccggg atgagcttgg cgagttctgc
     2821 gacgttctcc tcccagaagg gattgaagta cacgcaacgc gaaatcgccg ccaccggatc
     2881 ctcgccgtag agctgcggca tcttcttgtg ggtgtcgctg aggttctgaa ggaggcgggg
     2941 cagccaagtc gttccgttct ccaccatgcc gatgcggagc tcgggaaacc tcgtgaggag
     3001 tccgtggcag gccatcgccg cgacggcgtc ctcggccggg ttgcgagcca gggagtagta
     3061 ggagcggaat ggtgagggct ggaaggggcg gaactcgccg gccccctccc agtcgttggc
     3121 gtagcgggag tagccgctat cggaggcgtg catgatgacc aagacgtcgg tctcgaccac
     3181 tcgcttccag aaggggtcga actcgggcag ggcaaacgag cgcagaccgt gcaggccggg
     3241 gaccggcgcc ggccggatga gaatggcctg cgcaccgtgc tcgaccaccc aattcagttc
     3301 gtcgatggcc ttctccacga tcggcaaggt gatgatcggc gtggtgaaga tccggccctt
     3361 gtagtcgaaa ctccatgtct cgtgcagcca ctggttcagc gcatgaatga ttgtgtgtgt
     3421 cgcctcgggg tgttcgcgaa aacgctcttc caccaggctt gccagcgtcg ggtacatcat
     3481 tgatttgtcg atgccctgtt cgtcgagcaa ggccagccgg ggcgccggct cacgcatcgc
     3541 gggggtcgac tcgatcgcct tgcccagaat ctcgcggtgc gatttgcctt cgggattgcc
     3601 gttgtggaag tagtcctcct gggctcccgg cgcggcgacc ttgtcgaagg tcgggttggg
     3661 gatgtactcg ctgatctgac ccagcgtggc gatcttcgtg cggccgtcca cttcgacgta
     3721 ccggacggcg ccgtggtagt cgggcggcaa gaacttcgtc agtgcgtcgc gggtctcata
     3781 catgtggtta tcaccatcga agaccggaaa tcccaatcgc gacgtcattg aattcctcct
     3841 cgtgccgggt caagtctgcg atgcagcagg accactcgtt tatctcgtct ggacaatcgg
     3901 atgtgcgact gactctagca tcgagtgcgt taaaagagga catgccttta cttttctgtc
     3961 gtgtatcggt ctagctgagg gtaacgtggc cttctgctgc ggcacgatcg tcggtgaacg
     4021 gcgagtgctg tgcaacgtct ggaaggagga gtaagtggat ctgggtctta cgccggagca
     4081 acgcgcggtc tgcgatgcgt tcgcagccct tttcaagaaa caggccacgc ccgagcgagt
     4141 gcgccgagcg gagtcaactg gcttcgacga gctcctttgg cggcactacg ccgatatcgg
     4201 cgcgctgggc atcggggtgc cctcggactg cgggggcggc gacggtggtc tcctcgaact
     4261 ggcgttggta gccgaggaag ccggcggccg cctcgccccg atccccgtcg ccgagacttc
     4321 agcagcggcc cggacgctgg gtcgcctagg ggagaaggaa ctgctggaac cggtcctgac
     4381 cggatcggtc atcgtgtcgc tggccacccg tgccggcccc gtatctcagc agatgctggt
     4441 cgacggtgcg atcgcggacg cggtgctcgt cctcgacgga gacaagctgg tctatgtggc
     4501 gcggcccgtc ggcctagcca aggtggcgcg ccgcaacctc ggcgccctcc cgctgggaaa
     4561 gtgggacgac gccccgggcg ccacctctgt cctggccgag ggcagcgagg cgcgcatggc
     4621 gttcgacacg gctgtcgacg aggttcgcgt gctccgggcg gcggcgctcg tcggattggc
     4681 caccgaggcc gtcgggatcg gctcccgcta cgcgatcgtg cgcgaggcgt tcggccagcc
     4741 gatcgggatg taccaagcgg tcgcgcaccc cttcgccgat gccatcgcgg ctcgtgacgg
     4801 ggcgcaactg ctggtccgca aggcgtgttg ggcgttggac tccggtcacg aagacgcggg
     4861 cgcactggcg gcgatggcat tcgtgttcgc cgccgaaacg gcccatcacg caacggcgca
     4921 cagtctgcac gtgcacgggg gatacggatt catggaggag tacgacattc agctctacta
     4981 ccgacgagcc aaagcgtggg cggcggcgtt cgccgatccg cgacgcgagc tgctcaccgt
     5041 cgccgaccga cggttcggcc ccgcgacgac cggaggtcga tgagccgatg gacttcaccg
     5101 aaccccagaa ctggaagcga atccgccgcg aggccgaggc gttcgtcgcc gcccacgtca
     5161 cccgcgaagt gatcgaccgc gagcgcagga ccggcgacgg cgtcgatcgc gcgctcaccc
     5221 gcaagctggg ggagcgtggg tggatcgcct cgggatggcc cgtcgccgaa ggcggcgccg
     5281 ggctcgatcc gttcgaggcc gccgcgctgt gggacgcact gcgccacggc ggagtgccga
     5341 ccatcggacc cggcaccacg atgctgcccg ccaacgccat ccgcgccaca ggctccgagg
     5401 aactcaaggc ccgaatcctg ccgggcgtcg ctgccggcga gattttgatc tgcctgggat
     5461 acaccgaacc ggcgggcggc tccgacgtat tcgcgtgcgc gacccggtcg caacgtgacg
     5521 gagacgagtg gatcgtcaac gggcagaaga tcttcacgac gttcgcccac ctggccgact
     5581 actgcttcct gatgacccgc tcgcagcccg gatcggcggg aacgcggggc ctgacgatgc
     5641 tgctggtgcc gctggactca ccgggcatcg agatccaggc ggtgcacacg atggggcagg
     5701 agcgcaccaa catcgtgttc tacaacgacg ttcgggtcgc cgatgcgaac cgggtgggcg
     5761 aggcacacga gggttttgcc gtcatgcggg cggcgctgga agccgaacag aatgttgcgc
     5821 cggcctcgcg cacgggatgg gtcgcccgag acgcgctgga gttcgcccgc acgcatcgcg
     5881 gccccgatgg cgccgagttg atcgacgacg tcctggtccg cgaacgactg gcccggatgg
     5941 cgatggaagc ggaggtggcc gacctgctgg gcttgcgggt ggcattcctg gacgccgagg
     6001 gagaaagacc cggccctgta tcggcgttgt tcggaccgga gagttacgta cgggcgtcgg
     6061 cagcggcaat cgacattgca gggagcgcgg gcatgctcga ctggaccgat ccgcagagcg
     6121 cggtcgacgg cagcctggac gcgcactatc gcagcgccgt cgcgacgacc atttacggcg
     6181 ggtccagcga ggtactgcgc agtttgatcg tcgagaaccg gctcggattt ccgcgcagcc
     6241 gccctcggcg ctgacggccg gttcggcgaa agctatacgc gctcagagga agtcgctgcc
     6301 gccgtcgacg ttgaggtgag cgccggtcat gtaggtgttg gcgcgcgacg cacagaaggc
     6361 gatgacggcg gcgacctcct gcggcagtcc gaaccgaccc aggtccaccg acatgccgtg
     6421 atcgcgcccc agcagcgcga acgcacccac tgggtcagtc ggctcgaccc cggcggccgc
     6481 gagcataggc cgcgcccaat cggtgagaat cggacccgga gcgacggcgt tcacgaggat
     6541 ggcgtcgccc gcgaggctgc gggcgaggtt tttcgtgaca ctgagcaacg cgctcttggc
     6601 cgcggtgtag ctcaccaagc ccgccgactg gtgctgcacc gacatcgccg ttacattcac
     6661 cactcgggcc cacggcgcgg cgcgcaatag cggcagcgcc gctcgcacag ttcgcacagc
     6721 ggacaccacg cctgagtcga atgccgccgt ccacagcatg tcgtcgagat cctcgaagcg
     6781 accggcccct gctggcccgg cagcgttgac cagcacgttg agggcgcccc accgtgcgcc
     6841 gacggtcgcg aaggcgtcct cgacgtcgga tggcgagccc agatccgcct tcagcgtcag
     6901 cacctcggga ctgccggcgg ccgctagagc ggtgccggcg gcggccagtc ggttgtcatc
     6961 cctgccgatg atggcgaccc gtgcgccctg ttcggctacg cacgcagcgg tcgccagtcc
     7021 gataccgcgg gtgccgccgg cgatgacggc ggtggcgtcg gcgaatccaa ggtccacgag
     7081 gcccgctcta agcgaccaca cgtcggtcga actggcgggc aagccagtcc gcgctgtcgc
     7141 gtgccgcggt tcgagcgacc gcggtgcttg cgaacatgtc gaagccgtga actgcgcctg
     7201 cgtagacgtg cagttcggtg ggcacgcccg cccggcacag tcgagccgcg aactcgatgg
     7261 attcgtcacg caaacagtcc accgcgccca caccgacgaa tgtcggtggc agcccacata
     7321 attcggttgc ccgggctgac gccgcatccg ccggcacgcc gtcggtgtcg tagcggtcgc
     7381 cgagatatgc cgaccagccg aacgcattgg cttcgcgact ccaaaccggc aatctttcga
     7441 gttggctgga cacggtcagg ccgcggtcat caagcatcgg gtactccaga tactggaaca
     7501 gcagcggcac ctgcccgcca tcgcgccacc gcattgccag gctcgcggca agcgcaccgc
     7561 ccgcgcttcg gccggcgatc ccaacccgcc gcggatcgat ctgcagatca acggcgtggt
     7621 cgacgatgaa gcggagcccc tcgtcgcagt cgtccagcgg cgcggggtaa ggcgcttcgg
     7681 gtgccagccg gtattcgacg ctgacgcaca cacagcccag ccagcggcac cactccatca
     7741 gccgggtgtc gtccatgtgc cggtttccga tgaccatccc gccgccgtgc atccagtaca
     7801 cggcaggcgg cgtgtcgccg agctcgatgg gtcgaagaac agagagcgcg acgtctccgc
     7861 gttcggtgat cagctcggtg cgctcgatgt cggatgcgga ctgcggctcg aacgtgcttc
     7921 cggcgcgcat ggcggcgagg ttctcggcgg agaagtcgaa atccgggaaa ccgcgaagac
     7981 attcggcgat ctccgggtcg atccagttcg cgctgttcac cgtcatccac cgacacccat
     8041 caggtgcctg cccgcaccca cgagcccgcc gtcgacgcgg atgtcgcagc cggagatgaa
     8101 cgccgcgccg tcggactcga gaaaggccac agccgccgcg atgtctccgg gccgcccggg
     8161 tagcggcctg tccggacgtt tcaccggcgt cacctcgacc atgacgggca caatgggctg
     8221 acgatccagc tccagcctgc ccatcggcgt gtcgatcagg ccgggtgaga tcgagacggc
     8281 gcggccgccg gtcttgcccc acggcgtggc caggcgttcc accaaccgga tcacgccgcg
     8341 tttggcgagg acataggccg tggcactgtc cagcggctgg ccgagtgcct gctcgatcgc
     8401 cgcgagaaaa ccgtcagcca gcggatcgtc gaggaacggc tccaccgccg cgggaacatc
     8461 gctgtagccg gcgatggatc cgatcaggat cgcggagctg cccgggttga ccagggggaa
     8521 caaggcgtcg gtgatgcgaa ccgacccggc cagatccacg tcgagcaccg tggccgcatc
     8581 ggccatctcc ggtgaaacgc ccgcggtatg cactaggcag cgcaggcggc caagcccgct
     8641 cacccgatcg gcgaccgcgg cgacatcagt cgcagaggtc acgtcacagg acagcgtgtc
     8701 cacgaccgcc ccctgcgcgc ccagggcctt gcgcgcctgg tcgagcagat cctcgcggag
     8761 atccaccagc aacagacgcc cgcgatcggc gagtgcgcgg gcacaggcca gccccatgcc
     8821 gcccgcaccc ccggtgatca ccagaatgtt gtcaacagcc atcgtgccct ctcgttgttg
     8881 aaagtccttc gccgcctcag agccgttgaa tgatggtcgc gttcgccatc ccgcccccct
     8941 cacacatggt ctgcaggccg tagcggccgc cagtggcctc caggtgattg accagggttg
     9001 ccagcagccg ggttccagag gaccccagtg cgtgtcccaa cgcgattgct cctccccacg
     9061 gattgagccg ctccggatca gcaccaagct cctgcgccca ggccagtggc acacaagcaa
     9121 atgcctcgtt gacctcgtat gcatcgatat cgtcgagcgt caggcccccg cggtgcaatg
     9181 cccgttgagt ggccggaatg ggtgcggtca acatgtagag cgggtcgtcc cctgcgacgg
     9241 agaacgcatg aaaccgcgcg cggggccgca ggccagaggc gagtgccatc cgctcgctca
     9301 tgatcaacgc cgccgaagca ccgtccgtca gtggagaaga gttcccgggc gtgatccgcc
     9361 actcgaggac gggaaagcgc tcggccgctt cggtcgaggc gaaggacggc ttcagaccgc
     9421 tgagcgcctg cgtggtgctg tcggctcgaa tggtctcatc gacggcgtgc gtaccggggc
     9481 ggccggtcgc gtcgacgacc ggcatcgcga cgatctcgtt ggcgaaacat ccgtcggctg
     9541 ccgcggccgc ggcccgttga tgtgaggtga cggcgaaaga atccagcgct tcctggtcaa
     9601 gcttccatcg ctccgccacc agttcggcgg ccaccccctg tttgaccagg ccctgcggat
     9661 accgcgccgc gacgctcggc ccgaaggtat cggcgcccga tgatgctgtt cccatcggaa
     9721 cccggctcat ggactcgacg ccgcaggcga tcacggcgtc gtacgcgccg gccatgacgc
     9781 cctgggcggc gaagtgcaca gcttgctggc tggaaccgca ctggcggtca acggtcgtgg
     9841 ctggcacgga ctccggaaag cctgcggcca gaaccgcgct gcgggcgatg ttgaagctct
     9901 gctggcccac ctgctccacg catccggcga tgacatcgtc gatacagccg ggatccaaat
     9961 cgtttcgggt caggatctcc accagcacct ggcgcagcaa gtcgacgggg tggcagcccg
    10021 acagtgcgcc accgggtttg cctttgcctg aggccaaccg gacgacatcg acgacgacgg
    10081 cgttgttcat tctctaacct ccgctggcgt agacacctgc cgatggccga gtcgaccgca
    10141 ccggtagggc aagtgtgcca catggcgaac ccatgacatg cattcagaga gaacctaatt
    10201 gtagtagaaa gaaagtgatg gtctactgag tccatgaagg tgacagtcga tcccgatctc
    10261 tgtgaagcaa atgcattgtg cgtggcagaa gcccctgaag tgtttgatct gagcgacgat
    10321 gaggtcgtgc acatcctcct cgcgaaaccg cccaccggac tggagccatc ggtgattgag
    10381 gcggtcagcg cctgtccgaa acaggcatta cggatcgtta cggactgaat gagtagccca
    10441 gttccgctcg agccagcaca tctgcgttgc agaaaacgaa ctcgtcgttc tacgctgggg
    10501 aaggtcggtt cttacctctt gtgagagccc gcgtgcctat cttctaggag ggaacctcgc
    10561 atgctgcccg acgacgccaa gatcatctcg gtcgacgacc acgtcatcga gcacccgcgc
    10621 gtctggctgg accgggtgcc ggccaagtat gcggaggtag caccgcgcat cgtcaagctg
    10681 cccgacggca atgacacctg gctctacgag gggaagcagt ccggcaactt cgcgctcaac
    10741 gccgtcgcgg gcaagcaccc ccgggatttc gggatggacc cccgcagcta tgacgacatg
    10801 ctgccgggct gccatgacat cgaggaccgc atcagggaca tggacatcga gggtgtctgg
    10861 gcccagttgt gcttccccaa cttcggtggc ttcgccggaa gcaccttcca tacggcgacc
    10921 gacaaagatt tcgcgaagat atgcatcagc gcctacaacg acttcatcct cgatgagtgg
    10981 tgctcctatg cgcccgagcg ccagattccc ttgatgatgg tgccgttctg ggacatcgag
    11041 gcatctgtcg ccgagatcga gcggaccgcc gccaagggtg ccaaatccat ctccttcatc
    11101 gaggcgcctc acaagatcgg tctgccgtcc tatcacaccg accactggga tccgatcttt
    11161 cacatctgcg aggaggccgg cctaccgctg tcgatgcact tcggttcagg cggtatgccg
    11221 cagggacttg ctcccgacgg tgacctgttc atcggtatcg cgctgttcgg gctgaattcg
    11281 atgatggcca ccgtcgacct gctgatgtcg gacgtctttt acaagtttcc caacctcaag
    11341 gtcgcccttt ctgagggcgg tatcggatgg atgccatacc tgctcgaacg catcgactac
    11401 tcctggggtc gccacaagta ctggtgcgac atcaatcccg acaaacttcc ctcggatttg
    11461 ttccgggagc acatctacgg atgcttcatc tccgaccagt ccggcatcga gcagcgccac
    11521 cgaatcggca tcgacaacat catgttcgaa agcgattacc cccattcgga ttccaactgg
    11581 ccgcacacgc gcaagctgct cgccgagcac ctcgtcgatg tgcccgacga agaggcgcgc
    11641 aagatcgtcg agctgaatgc ccggaccctg tacaacttct gggcctgaga ttcgatgcct
    11701 gacgcattca tcctttcagc cgccagaaca ccgattggcc gggcccgcaa gggatccctg
    11761 gtttccgtgg acgcctacca actcgccgag gtcgccgtcg gcgcggcagt gcaacggtcc
    11821 gagatcgcga tcgctgagtt cgacgacctc atcctcgccg agtcgttaca gggtggcggc
    11881 gtgatcgcgc ggaacatcgc ggtgcgtctg ggtatgacca gcgtgcccgg ggtggccatc
    11941 aaccgacact gcgcctcggg cgccaccgca gtgcagttgg cggcggccac catcatggcc
    12001 ggcatggcag acgtgatcgt cgccggcgga acagaaagcg ccagcaccat gcctcggctc
    12061 acgaaaatgg cgcccggggc gcaggagccc accccctggt cgccgcagag ccaccccgat
    12121 gcccccgggg tgcctgcctt cgacatgtcg gtgaccatcg gagagaacac cgcccggctg
    12181 catcacgtga cccgcgagca ggccgacgcc tggtccgcac gctcacatca acgtgcgctc
    12241 aacgcgatcg ccaaagggta cttcgacgac gagatcgtcg ccgtccccgt cggtcacggc
    12301 gcgacgaccg tcatgttcgg caccgacgaa cacccgcggg acaccacgcc cgaagcgctc
    12361 gccgggctga aggtcctaca cccggaaatg cccggcgccg tcgtcacggc cggcaactcc
    12421 gccggcatca acgacgccgc cgcagcgctg gtcatcggga gcgccgactt cgcggccagt
    12481 cacggcctgg ccccgctggc gcggatacgc gggtggacgt ccgtcggcgt ggaggtcaag
    12541 cacaccggga tggccccggt gacggcaatc ccgctggcgc tcaagcgtag tggtctcaca
    12601 ctcgatgacg tcgacctcgt cgagatcaac gaagccttcg cgacgatggc ggtggcgtgt
    12661 acccgtgacc tcggtttgga cgaatcgatc gtgaacgtca acggaagcgg catcagtctc
    12721 ggccacccga tcgcagcgac cggcgcccgc atggtcgtat cgatcattca tgaactcgca
    12781 cgacgcgatt cgcggatagg cgtggtggcg atgtgcgccg gcggaggcat gggctccgca
    12841 atggtagtcg aacggatcta gggtcagccg aaccggtcag cgccgcaccg cgagtttggt
    12901 gtagcgcccg ccgagcaacc attcagtgac gtagaggttt ccgtctgcgt ctgttgccac
    12961 tgcatgtggt gaattaaagc ggtcgtgctg aggcagatcg ggtatctcag ctcgcccgtc
    13021 gtcggccagg tcattgggcc aacccggtcg ggtcggccat ccctgcgccg cttcgggatc
    13081 cgggttggcg ccgatgtatc cgacgaggtg atcctcggaa tccagaacgg ccaggcgctc
    13141 atagatctcc gcgaccacca gtaggtcgcc ccatcgcgcg aagtcgcttg gactgttcag
    13201 gaaggcctcg ccaaacgtgc gcaggtagcg gccctccagg tcg
//
LOCUS       DASYLX010000805         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3226017,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000805 DASYLX010000000
VERSION     DASYLX010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226017"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VGP27_18790"
     CDS             complement(<1..1372)
                     /locus_tag="VGP27_18790"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7853381.1"
                     /translation="MESTDLAFAGAAEQARLLAAGTVTAPALLDLYLERIARLDPELR
                     SYRVVLAESARREAAAAQARLDNGERLPLLGVPIAIKDDVDVAGQVTTYGTSAYGPAR
                     TADAEVVRRLRAAGAVILGKTTVPEMMIYSFTETLEFGATRNPWNTNRTPGGSSGGSG
                     AAVAAGLASMALGSDGMGSIRIPSTWCGLFGIKPQRDRVPLAPHDGAWNGLSVNGPMA
                     RTVEDAAVFLDVTSTTPAAEGSFVSAAGRDPGRLRIALSTKVPPLLTARVGKAQRAAV
                     DEAAALLRELGHQVVARDPDYPVGAVYGHLLPRYFRGVYDDVETLPRKERLERRTRAF
                     ARIGGLISERRMAAIRGAEAELAQRVLSIFDDVDVVITPGTAAGPSRIGAYQGRGAVS
                     TLTLVAQRVPFQAMFNGTGQPAAVVPWSLDGEGLPTSIQIVGRPFDEATLLSLGAQIE
                     KARPWAH"
     gene            complement(1391..>1681)
                     /locus_tag="VGP27_18795"
     CDS             complement(1391..>1681)
                     /locus_tag="VGP27_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853382.1"
                     /translation="IRDDDRLHRVEQVMGLSPWSARTTQRVFRRYVGVPVKWVLCRYR
                     LQQAALEIESHPGVDFADLAVRLGWYDQAHFINDFRSMLGSTPGEYASAHGC"
BASE COUNT          266 a          597 c          557 g          261 t
ORIGIN      
        1 ggtgcgccca cggtcgtgcc ttctcgatct gcgcgccgag ggacagcagc gtggcctcgt
       61 cgaacggcct gccgacgatc tgtatcgacg tcggcaggcc ctctccatcg aggctccacg
      121 gcacgactgc ggccggctga cccgttccgt tgaacatggc ctggaacggc accctctgcg
      181 cgaccagtgt cagcgtcgag acggcaccgc gtccctgata agcgccgatg cgcgacgggc
      241 ccgctgccgt gcccggggtg atcaccacgt cgacgtcatc gaagatcgac agaacacgct
      301 gcgccagttc agcttccgcg ccgcggatag ccgccatccg ccgctcggag atcaacccgc
      361 cgatgcgggc gaacgcgcgg gtgcgccgtt ccagccgctc cttgcgcggc agcgtctcca
      421 catcgtcgta gacaccgcgg aagtagcgcg gcagcaggtg tccgtagacc gcaccgaccg
      481 gatagtcggg gtcgcgcgcc acgacctggt gaccgagttc acgcagcagc gccgcggcct
      541 cgtcgacggc ggcccgctgg gctttgccga cccttgccgt cagaagtggg ggaaccttgg
      601 tgctcaacgc aattcgcaat cgtccgggat cgcggccggc cgcggagaca aaacttcctt
      661 cggcggcagg cgtcgtcgac gtcacgtcca ggaacacggc ggcgtcctcg acggttctcg
      721 ccatcggacc gttgacggac agaccgttcc acgcgccgtc gtggggtgcg agcgggacgc
      781 ggtctcgctg cggcttgatc ccgaacaggc cgcaccacgt cgacgggatc cgaatcgaac
      841 ccataccgtc ggaacccagc gccatcgatg ccagacccgc tgcgaccgcg gcgccgctgc
      901 cgccgctgct gccacccggg gtgcggttgg tattccacgg atttcgagtc gcaccgaact
      961 cgagcgtttc ggtgaacgag tagatcatca tctccggcac ggtggtctta ccgaggatga
     1021 ccgcgccggc cgcgcggagc ctgcgcacca cctcggcgtc cgccgttcgg gcgggcccgt
     1081 atgcgctggt gccgtacgtg gtcacctggc cggcgacgtc gacgtcgtcc ttgatcgcga
     1141 tcggcacgcc gagcagcggt agccgctcgc cgttgtccag ccgagcctgc gcggcggccg
     1201 cttcccgacg cgcggactcg gccagcacga cgcggtagga ccgcaactcc ggatccaatc
     1261 gcgcgatgcg ctccagatac aggtcgagca atgcgggcgc cgtgacggtg cccgcagcga
     1321 gtaggcgcgc ctgttctgcg gcgcctgcga acgccaggtc ggttgattcc actggcggca
     1381 gcgtatcggg ctagcagccg tgcgcgctgg cgtattcgcc cggcgtactg cccagcatcg
     1441 aacggaagtc gttgatgaaa tgtgcctggt cgtaccagcc gaggcggacc gccagatcgg
     1501 cgaagtcgac gccgggatgg ctttcgatct ccagcgccgc ctgctgcagc cggtaccggc
     1561 acagcaccca tttcaccggt acaccgacat agcgccgaaa cacccgctgc gtggtgcggg
     1621 cactccatgg cgagagcccc atcacctgct ccacccggtg cagacgatcg tcgtcgcgga
     1681 t
//
LOCUS       DASYLX010000806         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2567729,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000806 DASYLX010000000
VERSION     DASYLX010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2567729"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGP27_18800"
     CDS             <1..318
                     /locus_tag="VGP27_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853383.1"
                     /translation="PTELDYDGIAAKVAAVLGFPVRYEPVDIDRFAEVLRLEGHGDYF
                     VQHITNVAQDYRDGIFAGTNNLVEVITGTPALTVEQFAAANRDAFATAGQAPAWEKAR
                     VAR"
     gene            364..783
                     /locus_tag="VGP27_18805"
     CDS             364..783
                     /locus_tag="VGP27_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7853384.1"
                     /translation="MATMSIEDRERFLADLHVGVLAVERPDRAPLAVPIWYAYEPGGE
                     LSIWTSEGTLKDKLIRAAGRFSFTVQNEEPPYKYVTVDGDVTSVGPADETEARKIAIR
                     YLGEEAGNQFTDENHSPSSIVIRMRPKKWLSVDYSAE"
     gene            complement(907..1440)
                     /locus_tag="VGP27_18810"
     CDS             complement(907..1440)
                     /locus_tag="VGP27_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853385.1"
                     /translation="MGLFFLGVMIVAAYGYTPYIKIGRKVYALTIQNREIESDERSVQ
                     SSDTVASDKDDGLAPDSYSGLLTSTTMWWLLIPLLVISTGNIYAFAFSTGEWWVAAIG
                     LVFLVFLAVVLGFGDASWGHGIARGRRVQFAIAAVVTVGVFAALYVAAYQAGKHWPMR
                     RKQSMEYRTQPRHQKLD"
     gene            complement(1616..2134)
                     /locus_tag="VGP27_18815"
     CDS             complement(1616..2134)
                     /locus_tag="VGP27_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853386.1"
                     /translation="MPLTLERFATQLQNAGVSPQGAQQAINEIQNGASPGQVIRDVSA
                     GLASQAGNLGEGAKAHAGAMPGGGHWGTAPVWSNADAEALKGFGSKLGTAGTVIDGLL
                     TVNDVLHGAPAGEEAAQFGGRTLGSMGGGWLAGAAWGSLVGPEGTLVVGFLGALGGGI
                     GGEKFVNWMMGK"
     gene            complement(2193..2990)
                     /gene="istB"
                     /locus_tag="VGP27_18820"
     CDS             complement(2193..2990)
                     /gene="istB"
                     /locus_tag="VGP27_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEV7853387.1"
                     /translation="MATTKTAAARDVTAELGYLTRALKAPTLRDSVARLAERARAENW
                     SHEEYLAACLQREVSARESHGGEGRIRVARFPARKSLEEFDFDYARGLKRDTIAHLGT
                     LDFVTARENVVFLGPPGTGKTHLAIGLAMRACQAGHRVLFATAAEWVARLADAHHAGR
                     IQDELTRLGRYPLLVIDEVGYIPFEPEAANLFFQLVSSRYERASLIVTSNKAFGRWGE
                     VFGDDVVAAAMIDRLVHHAEVIALKGDSYRLKDRDLGRVPAAGTTEE"
     gene            complement(2990..4231)
                     /gene="istA"
                     /locus_tag="VGP27_18825"
     CDS             complement(2990..4231)
                     /gene="istA"
                     /locus_tag="VGP27_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEV7853388.1"
                     /translation="MLSVEDWAEIRRLHRAEGLPIKAVARVLGVSKNTVKAALAAEGP
                     PRYERSPKGSIVDEVEPRIRELLQGYPMMPATVIAERIGWTRSMRTLSGRVAELRPVY
                     LPPDPASRTAYVAGEIAQCDLWFPPITLPVGSGQTRRPAQLPVLTMVCGYSRWLSGRL
                     IPSRRSQDLFAGWWRVIEKLGAAPRVLVWDGEGAIGRWRGGRTELTAECQAFRGTLAA
                     KVIVCRPADPEAKGIIERAHDYLERSFLPGRVFASPADFNTQLEGWLAVVNTRNRRVL
                     GCAPTDRIAADRHAMLALPPVAPATGWRASVRLPRDHYVRLDGNDYSVHPSMIGRRVE
                     VIADLDRVQAVCDGRSVADHERIWATHQTISDPAHVEAAKVLRRSRIGTVRPAPELDV
                     QVRSLTDYDAALGLDTDGGVA"
BASE COUNT          837 a         1542 c         1377 g          749 t
ORIGIN      
        1 ccgaccgaac tcgactacga cggcatcgcc gcgaaggtcg cggcggtact cggcttcccg
       61 gtccgctacg aacccgtcga catcgacagg ttcgccgagg tgctgcgtct ggaaggccac
      121 ggtgactact tcgttcagca catcaccaac gtggcgcagg actaccgcga cggcatcttc
      181 gccggaacca acaacctcgt cgaggtgatt accggaactc cagcgctgac cgtggagcag
      241 ttcgccgcag ccaaccgcga cgccttcgcg accgccggcc aggcaccggc gtgggagaag
      301 gcgcgcgtag cccgctgacc cgaccgaggg aatgtgggcc gcgtcgctcg ggttggcacg
      361 gtcatggcga cgatgagcat cgaagaccgc gaacgcttcc tggccgacct tcatgtcggc
      421 gtactcgcag tggaacggcc cgatcgtgca ccattggcgg tgccgatctg gtacgcctac
      481 gagcccggcg gcgagctgtc catctggacc agcgagggga cgctgaagga caagctgatc
      541 cgcgcggccg gacgattctc cttcaccgtc cagaacgagg aaccgcccta caagtacgtc
      601 accgtcgacg gcgacgtgac gtccgtcggg cccgcagacg agaccgaagc ccgaaagatc
      661 gccatccgct acctcggcga ggaagcgggc aaccagttca ccgacgagaa ccactcgccg
      721 tcctcgatcg tcatccggat gcgacccaag aagtggttga gcgtcgacta ctcggccgag
      781 tgacgagctg acgacttaga tccagtctgg ctcgtcgcag cgccgttacc aaaagcgtcc
      841 gctcggccga agcgacgagc aacgtcgcga tcgacatcca ggtcgaggcg cgcatcgttg
      901 tggctgtcag tccagttttt ggtgccgcgg ctgggtgcgg tactccatgg actgtttgcg
      961 tcgcatcggc cagtgcttcc ccgcttgata agccgccacg tagagcgccg caaacactcc
     1021 cacggtgaca actgccgcaa tggcgaactg cacgcggcgg ccacgggcga tcccgtggcc
     1081 ccaactcgcg tcgccgaagc ccaagacgac ggccaggaat acgaggaata cgagcccgat
     1141 agctgcgacc caccattcgc ctgtgctgaa tgcaaatgca tatatgttgc cggtcgagat
     1201 gacaaggagt ggaatgagca accaccacat tgtcgtggat gtaagcagtc cgctgtaact
     1261 gtcaggagcg aggccgtcgt ccttgtcact cgcgacggtg tcggaactct gcaccgagcg
     1321 ttcgtccgat tcgatctctc tgttctggat ggtgagggca tacaccttgc ggccgatctt
     1381 gatgtagggg gtgtacccat acgccgcaac gatcatgaca cccaggaaga acaggcccat
     1441 cccgaatcct ttttcggaat cgggatagaa cgcgaggaac gccgcagtgg caccgagact
     1501 tgcgcagctc cagtagatgc gccgttgcaa gctgaggctg tacttgacgt actgctgtat
     1561 cagggcaaca accgcgcaag cgacgagaat aatgaaaaat acatttgaca ccgtcttatt
     1621 tgcccatcat ccagttcacg aacttttctc ctccgatacc cccgcctagc gcgccgagga
     1681 agccaacaac cagtgtgcct tcggggccaa cgagagaacc ccaagcagca cccgctaacc
     1741 acccgccgcc catcgatccc aaagtacggc caccaaattg cgcggcctct tccccggcgg
     1801 gagcgccgtg aagcacgtcg ttgactgtca gcagtccatc gataacggtg ccggccgttc
     1861 ccagtttgga accgaacccc ttgagtgcct ccgcatcagc gttcgaccac actggcgccg
     1921 tgccccaatg gcctccgccg ggcattgcac cggcatgcgc tttggcgccc tcgcccaggt
     1981 tgccggcctg ggatgcaagg cctgcgctca catcccttat cacctgcccc ggcgatgcgc
     2041 cgttctggat ctcgttgatt gcctgctggg ctccttgtgg tgacacgcca gcgttttgca
     2101 gctgcgtcgc gaatcgttcg agtgtcaacg gcaactgaag tctgacccct ttgcggcaag
     2161 tgaaaattga ccccctgggt cggtgttcat cttcattctt cggttgttcc ggctgcgggg
     2221 acgcggccga ggtcgcggtc tttgagccgg tagctatcgc ctttgagggc gatgacttcg
     2281 gcgtggtgga cgaggcggtc gatcatggcg gcggccacga cgtcgtcgcc gaacacctcg
     2341 ccccaccgtc cgaacgcttt gttggaggtg acgatcagac tcgcgcgttc gtagcgggac
     2401 gacacgagct gaaagaacag gttggcggcc tcgggctcga acgggatgta gccgacctcg
     2461 tcgatgacga gcagcgggta gcggcccagt cgagtgagtt cgtcttggat gcggccggcg
     2521 tggtgagcgt cggcgagtcg ggcgacccat tcggcggcgg tcgcgaatag cacccggtgc
     2581 ccggcctggc aggcacgcat ggccagcccg atcgccaggt gtgtcttgcc ggtgccgggt
     2641 ggcccgagga acaccacgtt ctcgcgggca gtgacgaagt ccagggtgcc caggtgcgcg
     2701 atggtgtcac gcttgaggcc acgggcatag tcgaagtcga actcctccaa cgatttccgt
     2761 gcggggaatc gtgcgacgcg gatacggccc tcgccgccgt gggattcgcg ggccgacact
     2821 tcgcgctgca ggcaggcagc caggtactcc tcgtggctcc agttctcggc gcgggcccgt
     2881 tcggccaacc gggccaccga atcgcgcagc gtcggcgcct tcaacgcccg cgtcaggtaa
     2941 ccgagctcag cagtcacgtc gcgagccgct gcggtcttgg tggtggccat cacgcgaccc
     3001 cgccgtcggt gtcgaggccc agcgcggcgt cgtaatcggt gagcgaccgc acctgcacgt
     3061 cgagttcggg tgcggggcgc accgtgccga tccggctgcg gcgcagtact ttcgccgctt
     3121 ccacgtgcgc ggggtcggag atcgtctggt gggtggccca gatccgttcg tgatcagcca
     3181 ccgaacgacc gtcacagact gcttgcaccc ggtccaggtc ggcgatcacc tcgacccggc
     3241 gcccgatcat gctcgggtgc accgagtagt cgttgccgtc cagacggacg tagtgatcac
     3301 gtggcaaccg caccgaggcg cgccaccccg tcgccggcgc taccggcggc aacgccagca
     3361 tcgcgtggcg gtccgcagcg atccggtcgg tcggcgcgca gcccaacacc cgccggttgc
     3421 gcgtgttcac caccgcaagc cacccctcga gctgggtgtt gaaatccgct ggggacgcga
     3481 acacccgccc gggcagaaac gaacgctcca gatagtcgtg cgcccgctcg atgatgccct
     3541 tggcctcagg atcggctggg cgacagacga tcaccttcgc cgcaagagtg ccgcgaaacg
     3601 cttggcattc cgcagtgagc tcggtgcgac cgccacgcca gcgtccgatc gcgccctcgc
     3661 cgtcccacac caacacccgc ggcgcagcac ccagcttctc gatcacccgc caccaaccgg
     3721 cgaacaaatc ctgcgatcgc cgcgacggaa tcaaccgacc cgacaaccac cgcgaatagc
     3781 cgcacaccat cgtcaacacc ggcagctgag ccggtcgccg ggtctgaccg gacccaaccg
     3841 gcagcgtaat cggcgggaac cacagatcgc actgcgcgat ctccccggcc acataggcag
     3901 tgcgcgaagc cggatccggc ggcagataca ccggccgcag ctcggccact cgccccgaca
     3961 acgtccgcat cgaacgggtc cacccgatcc gctcggcgat caccgtggcc ggcatcatcg
     4021 gatacccctg caacaactcc cggattcgcg gctcaacctc atccacgatc gaccccttcg
     4081 gtgaccgctc atatctcggt ggcccctctg cggccaacgc cgccttcacc gtgttctttg
     4141 aaacaccgag cacccgagcg accgccttga tcggcaggcc ctccgcccga tgcaatcgac
     4201 gaatctccgc ccagtcctcc acagacaaca tcccttcgtc ctcctggctc gactagaacc
     4261 aggtcaccgg accaaggggt caaaattcgg atgccacgca ggggtcagtt ttcacccgcc
     4321 gtcgacacaa ccgaagagca gagcgcaagg cagccctttc ggaggcctcc aagtcgggta
     4381 cttcgttcgt cggcaggggc gcacgctgac gggaatatcc cccgtatctc gccgctgcgg
     4441 ccctgcccca ggcggtcggg ttagccccaa aggccgggca agtggcctgc agctcgacct
     4501 ccgtg
//
LOCUS       DASYLX010000807         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_483593,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000807 DASYLX010000000
VERSION     DASYLX010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483593"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VGP27_18830"
     CDS             <1..726
                     /locus_tag="VGP27_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7853389.1"
                     /translation="ARDDNSGWDIETPAFETFYQSGVSVVMPVGGMSSFYTNWQSPAV
                     GNGQTYNYQWETFLTSELPAYLAANKGISQSGNAVVGLSMSGNSALILAAYHPNQFRY
                     AGSLSGFLTSSQGVWPTLIGFAMRDSGGFDATAMWGPGGGPAWQRNDPALQAGKLASN
                     GTRLWVYTGNGTPTELGGADLPATFLEGLTKDSNVAFQNAYTAAGGRNATFNFPPNGT
                     HSWGYWNAQLTAMKPDIQRTLGA"
     gene            complement(731..1858)
                     /locus_tag="VGP27_18835"
     CDS             complement(731..1858)
                     /locus_tag="VGP27_18835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV7853390.1"
                     /translation="MRVAIVAESFLPNVNGVTNSVLRVMEHLRHHCHEILVIAPDTPR
                     GDPPADRVHEGVRIHRVPSRMFPKITSLPLGVPRPRMVSVLKGFDPDIVHLASPALLG
                     WGGMHTARYLGVPTVAIFQTDIAGFAESYGIGFTAKAAWAWTRMVHSRVDRTLAPSTA
                     AMENLAAHRIPRVHKWARGVDIAGYAPSARDEQLRRQWSPAGKPVVGFVGRLAPEKHV
                     ERLAVLGRREDLQLVIVGDGVDRAKLEAALPTAVFTGALYGKELATAYASMDVFVHPG
                     EHETFCQTVQEAMASGLPVIAPDAGGPRDLVAPMHTGLLLPVPEFEDRLATSVDHLLA
                     ERQRYSLAARRSVLGRTWPAICDELIGHYQAVLGTRGVKAA"
     gene            1992..2699
                     /locus_tag="VGP27_18840"
     CDS             1992..2699
                     /locus_tag="VGP27_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV7853391.1"
                     /translation="MRIGRAAVAATAALLIGAVGCTNQIAGTATPDPTQPPLALSDDG
                     YGIVAGYPDAPVQIELYTEPQCNHCEELQSTYGQDIERSINLGELAVTYRPLTFLDTP
                     STDEHSARVANALFLAVGAPEATDEANMSSGLEFQRFVEDLWAHQEPGGPGPSDQQMA
                     KMAEDSGLPADVAGRVGDGEAAGNVNIGEMAAYNYGALIGIDPITTGTPTVYDLSTKQ
                     KVDLHDKDWLANLLSSV"
     gene            complement(2657..3196)
                     /locus_tag="VGP27_18845"
     CDS             complement(2657..3196)
                     /locus_tag="VGP27_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEV7853392.1"
                     /translation="MERVKRLVRRVVPQVIPHLYGEPGETHTQRTFRRIRIGVVLVAC
                     LVTLQSLLLVFGAWRNDRQIEGNMGVAAAEVLSAGPRRSTVEFVTPDRVTYRPELGVL
                     YPSELDTGMRIYVEYDKDNPDLVRVQDRNAALAIIPAGSIAVVGWLVAGAALTALTFF
                     ERRLSRQTEDNKLASQSLS"
     gene            complement(3196..4848)
                     /gene="menD"
                     /locus_tag="VGP27_18850"
     CDS             complement(3196..4848)
                     /gene="menD"
                     /locus_tag="VGP27_18850"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEV7853393.1"
                     /translation="MNPSTAQARVVVDELIRGGVRDIVLCPGSRNAPLAFALHDADRE
                     GRIRLHVRIDERTAGFLAIGLTLAEGAPVCVAMTSGTAVANLGPAVVEANYARVPMIV
                     LSANRPYELLGTGANQTMEQLGYFGTQMRENISLGLAPDLKSAAPGELDKFNAQWRSA
                     TCRVLVAATGSRTANAGPVQFDIPLREPLVPDRDSDPSTSYAPQGRPGGKPWTYTPPV
                     TFDQPLDIDLTPDTVVIAGHGAGAHPNLAALPTVAEPTAPPADNPLHPMALPLLRPQQ
                     VIMLGRPTLHRPVSALLADSSVPVYALTTGPRWPDVSGNSQATGTRAVITGEPNPAWL
                     RRCAAVNGQATEAVRGQLNAHPLTTGLHVAAAVADSLREGDQLVLGASNPVRDAALVG
                     LNAHGLHVRSNRGVAGIDGTVSTTIGAALAHDRTGGRTIALMGDLTFVHDSSGLLIGP
                     TEPTPRHLTIVVSNDNGGGIFELLEQGDPRFSDVSSRIFGTPHDVDVGALCRAYHVES
                     RQMEVGDLAAALNEPFDGMRVLEVKADRSSLRALHASIKAAL"
     gene            complement(4858..>5296)
                     /locus_tag="VGP27_18855"
     CDS             complement(4858..>5296)
                     /locus_tag="VGP27_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853394.1"
                     /translation="RHDRTRDFFRIAERELADAGIELPATYDAKLRLLESFSPKTLND
                     DHAVRDWIDMFTVWPQKPTPGLRAQLEVGPHDNRLPAYRSITTPVLVIGFSDDIALPP
                     HLGREVAEALPNGRYLEIADAGHLGFLEKPQEVSAAALKFFAE"
BASE COUNT          893 a         1778 c         1795 g          830 t
ORIGIN      
        1 gcccgcgacg acaacagcgg ctgggacatc gaaacgccgg cgttcgaaac cttctaccag
       61 tcgggtgtgt ccgtcgtcat gcccgtcggc ggcatgtcga gcttctacac caactggcag
      121 tcaccggcag tcggcaacgg ccagacctac aactaccagt gggagacgtt cctgacgtcc
      181 gagctgcccg cctacctggc ggccaacaag ggcatctcgc agagcggcaa cgcggtggtc
      241 ggactgtcga tgtcgggcaa ctcggcgctg atcctggcgg cctaccaccc taaccagttc
      301 cgctacgccg gctcgctgtc cggtttcctc acctcgtccc agggcgtctg gcccacgctg
      361 atcggcttcg caatgcgcga ctcgggcggc ttcgacgcca ccgcgatgtg gggtcccggc
      421 ggcggacctg cgtggcagcg caacgacccg gcgctgcaag ccggcaagtt ggcgtccaac
      481 ggcactcgcc tgtgggtcta cacgggtaac ggcaccccga ccgagctggg cggagcagat
      541 ctgccggcga cgttcctgga agggctgacc aaggacagca atgtcgcctt ccagaacgct
      601 tacacggcgg caggcggacg caacgccacc ttcaactttc cccccaacgg cacgcacagc
      661 tggggatatt ggaacgccca gctcaccgcc atgaagccgg acatccagcg cacgctgggc
      721 gcctgacgtt ctatgcggcc ttgacgccgc gggtgccgag cacggcctgg tagtggccga
      781 tcagctcgtc acagatggcc ggccaggtgc ggccgagcac gctgcggcga gctgccagcg
      841 agtagcgctg acgctcggcg agcaggtggt caaccgacgt tgcgagccgg tcctcgaact
      901 cgggtaccgg cagcagcagt ccggtgtgca tcggcgccac caggtctcgc ggcccacccg
      961 cgtcgggggc tatgacgggt aggcccgacg ccatcgcctc ctgcacggtc tggcagaagg
     1021 tctcgtgctc gccaggatgg acgaagacgt ccatgctggc ataggcggtg gcgagttcct
     1081 tgccgtagag ggcgccggtg aaaaccgcgg tcggcaaggc ggcttcgagc ttggctcggt
     1141 ccacaccgtc gccgacgatg accagctgaa ggtcctcgcg gcggcccagc acggcaaggc
     1201 gctcgacgtg cttctcggga gccagccgcc cgacgaagcc gacgaccggc ttaccggccg
     1261 gcgaccactg tcggcgcaac tgctcgtcgc gtgcggacgg tgcgtacccg gcgatatcga
     1321 cgccacgtgc ccacttgtgg acccgaggaa tgcgatgtgc ggcaaggttt tccattgcgg
     1381 ccgtcgacgg tgccagggtg cggtcgacac ggctgtgcac catccgcgtc catgcccatg
     1441 ctgctttcgc tgtgaagccg ataccgtagc tctccgcgaa acctgctatg tccgtttgga
     1501 atatagcaac ggtcggcacg ccgagataac gcgctgtatg cataccgccc caaccgagca
     1561 gcgcgggaga cgccaggtgg acgatgtcgg ggtcgaaacc ctttagcacg ctgaccatcc
     1621 gggggcgggg cactccgagc ggaagcgagg tgatcttcgg gaacatccgc gaaggcaccc
     1681 ggtgtatccg gacaccctcg tggacacggt ccgcgggcgg atcgccgcgc ggtgtgtccg
     1741 gggcgatgac aaggatctca tgacagtggt ggcggaggtg ctccatcacc cgaagcaccg
     1801 agttggtgac gccgttgaca ttcggaagga acgattctgc gacgatcgca acgcgcacat
     1861 caagatagtg gcatcggcgg ctgtctccaa ggttgcatcc ggggatacga cacgcaaagt
     1921 tctcgtgccg atggccacga ccatcgcggt cggcgggcgg gtagtctgcg caccaaggaa
     1981 ggacggttga catgcgaatt ggccgtgcgg ccgtggcggc gacggcggct ctgctcatcg
     2041 gcgctgtcgg ctgcaccaac cagatcgccg gcaccgcgac tccggatccg acgcagccgc
     2101 cgctggctct gtccgacgac ggatacggca tcgtggcggg ctatccggac gcacctgtgc
     2161 agatcgagtt gtacaccgaa ccgcagtgca atcactgcga agagttgcag tccacctacg
     2221 gccaggacat cgagcgctcc atcaacctcg gcgagctcgc ggtgacctac cgcccgctga
     2281 cttttctgga caccccgagt accgatgaac attcggcgcg cgtcgccaat gcgctgtttc
     2341 tcgcggtcgg agcgcccgag gcgaccgacg aagcgaacat gtcgtcggga ctggagttcc
     2401 agcggttcgt cgaagaccta tgggctcacc aggaacccgg cgggcccggg cccagcgacc
     2461 agcagatggc gaagatggcg gaggactccg gcctgcctgc cgatgtcgcg ggcagggtag
     2521 gcgacggtga ggccgcgggc aatgtcaaca tcggtgaaat ggcggcctac aactacggcg
     2581 cgctgatcgg gatagacccg ataaccaccg gtacgccaac ggtttacgat ctgagcacca
     2641 agcagaaggt cgaccttcac gacaaggact ggctggccaa cttgttgtcc tcggtctgac
     2701 gggacagcct gcgctcgaag aacgtcaacg ccgtcagcgc cgcccccgca accagccagc
     2761 cgaccaccgc aattgagccg gccgggatga tagccagcgc cgcgttgcga tcctgcaccc
     2821 ggacgaggtc ggggttgtcc ttgtcgtatt caacgtaaat tcgcatgccc gtgtccaatt
     2881 cggacggata cagcacgccg agctcgggcc ggtaggtgac gcgatcgggg gtgacgaact
     2941 cgaccgtcga gcgccgcggt cccgcgctga gcacctcggc cgcggccacg cccatgttgc
     3001 cttcgatctg ccggtcgttg cgccatgcgc caaacaccaa cagcagtgac tgcaacgtca
     3061 ccagacacgc caccagcacc acgccgatgc gtattcggcg gaaggtccgt tgggtgtgag
     3121 tctcgccggg ctcgccgtac aggtgcggga tcacttgcgg gacaacccgg cgcaccaagc
     3181 gcttcacccg ttcgatcaca gcgccgcctt gatcgatgcg tgcagcgccc gcagcgacga
     3241 ccggtcggcc ttcacctcca gtacccgcat gccgtcgaac ggttcgttga gggcggcggc
     3301 aagatcgccg acctccatct ggcgactttc cacgtgatac gcccggcaca gcgcgccgac
     3361 gtccacgtcg tgcggagtgc cgaagatgcg cgacgacacg tcggagaacc gggggtcgcc
     3421 ctgctcgagg agctcgaaaa tgccgccgcc gttgtcgttg gacaccacga tcgtcagatg
     3481 tcgcggcgtc ggctcggtcg gcccgatcaa cagccccgag ctgtcgtgca cgaaggtcag
     3541 gtcacccatc agcgcgatcg ttcgcccgcc ggtgcggtca tgcgccagcg cagcgccgat
     3601 cgtcgtggac accgtgccgt cgatgccggc gacaccgcgg ttggaccgca catggaggcc
     3661 gtgcgcgttc aaaccgacca gcgccgcgtc gcgaaccggg ttcgaagcgc ccagcaccaa
     3721 ctggtcgccc tcacgcaacg agtccgccac cgcggcggcg acgtgcagac ctgtcgtcag
     3781 cggatgcgcg ttgagctggc cgcgaaccgc ctcggtggcc tgcccgttga ccgcggcgca
     3841 gcgccgcagc caggccggat tcggctcacc ggtgatcacg gcgcgggttc ccgtcgcctg
     3901 cgagttgccc gagacgtcgg gccaccgggg gccggtggtc agcgcgtaca ccggcaccga
     3961 cgagtcggcg agcagtgccg ataccggccg gtgcagcgtc gggcggccca gcatgatcac
     4021 ctgctgcggg cgaagcagtg gcaacgccat cggatgcagc gggttgtcgg cgggtggggc
     4081 ggtcggctcg gccaccgtcg gaagggccgc caggttcggg tgcgcgcccg cgccgtgccc
     4141 ggcgatgacg acggtgtccg gtgtcaggtc gatgtcgagc ggctggtcga acgtcaccgg
     4201 cggggtgtag gtccacggct tgccgcccgg tcttccctgc ggcgcgtagg acgtcgacgg
     4261 gtccgagtcg cggtcgggca ccagcggttc gcgcagcggg atgtcgaact gcaccgggcc
     4321 ggcgttcgcg gtgcgagaac cggtggcggc gaccaacact cggcatgtcg ccgatcgcca
     4381 ctgcgcgttg aacttgtcca gctcacccgg cgccgcgctc ttcagatccg gggcgagccc
     4441 gaggctgatg ttctcccgca tctgggtgcc gaaatagccc agctgctcca tcgtctggtt
     4501 cgcgccggtg ccgagcagct cgtagggcct gttggccgag agcacgatca tcgggacccg
     4561 cgcgtagttg gcctcgacca ccgcgggtcc gaggttggcg accgcggtgc ccgacgtcat
     4621 cgccacgcac accggagcgc cttcggccag cgtcagcccg atcgcaagga atccggcggt
     4681 gcgctcgtcg atgcggacgt gcagccggat ccggccctca cggtcggcgt cgtgcagcgc
     4741 gaacgccagc ggcgcgttgc gcgatccggg gcacagcacg atgtcgcgca cgccgccgcg
     4801 aatcagttcg tcgacgacga cacgggcctg tgccgtcgat gggttcacca ctacagccta
     4861 ttcggcgaag aatttcagcg ccgccgcgct cacttcctgc ggtttctcga ggaacccgag
     4921 gtgtccggca tcggcaatct cgaggtagcg gccgttgggc agcgcctcgg cgacctcccg
     4981 gcccaggtgc ggcggcagcg cgatgtcatc gctgaacccg atgacgagca ccggggtcgt
     5041 aatacttcgg taagccggca gccggttgtc atggggtccg acctcgagct gagcccgcag
     5101 cccgggcgtc ggcttctggg gccacacggt gaacatgtcg atccagtcgc gcaccgcatg
     5161 gtcgtcgttg agcgtcttcg gcgagaagct ctccagtagc cgcagcttcg catcgtaggt
     5221 cgcgggcagc tcgatgccgg cgtcggccag ttcgcgttcg gcgatgcgga agaaatcacg
     5281 ggtgcggtcg tggcgg
//
LOCUS       DASYLX010000808         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_594050,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000808 DASYLX010000000
VERSION     DASYLX010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594050"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGP27_18860"
     CDS             complement(<1..195)
                     /locus_tag="VGP27_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853395.1"
                     /translation="MGRHSVARRRRISPSVVPAAVIAPYALLLAAGGDVSVPAPTTEA
                     DAPDQIVDVAARSVQVVASAP"
     gene            511..926
                     /locus_tag="VGP27_18865"
                     /pseudo
     CDS             511..926
                     /locus_tag="VGP27_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(895..2748)
                     /locus_tag="VGP27_18870"
     CDS             complement(895..2748)
                     /locus_tag="VGP27_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7853396.1"
                     /translation="MTQPPAGDDGVATISCGSCGTEVSAAAFCGACGAQMSERGGGRF
                     RVSAYAAAPGEHVVRLSVASSLFPHLPHRSRTAFRVAFAVLFLLLMALALLRWQAPLI
                     AISALGLPLVFVLYLYEADVYRDVTLRTLVPSAVLGIGLGVGWALATASLVSGFYDAS
                     LADADSRRLSLIVGIAVPVGGALLMTVPAVVMRLARPTRESLDGFVIGSVGAISFTAA
                     ATLIRLAPQFGTGVTAGDRPASVLLAQAVVQGVAVPLTAAAVGGSVGAALWFGRRKAI
                     MSSVLGALALYALLGLTEAIPALEGLHVGAHLLITVVALLGLRIALQYCLTHERHDPP
                     NPQAQVLCPHCDHVVPDMAFCPNCGVSAAAASRASRTARRTAVSAEAAAARPGYATPA
                     RLYEVAPLRHTTLRWLFATLSAGLAVVVASGVTASALATPPRPRYTCPPDCGRPPIGK
                     PIDTNPRFVSEDGEFSVQYPVPGAFYKATLYPDWVALEYLGGDTGSLDLWGEAAGKRT
                     PKQVVDQMIAEYYPDATIDYEIPNAMVGYEPGYGAVFDQYPQDSSGEYTRLRLVALCA
                     VKNDYALVASAIGPYHEFTPEFGSGHPSGANLGLALDMGKYVNSFRWRSRA"
BASE COUNT          537 a          937 c          915 g          417 t
ORIGIN      
        1 cggtgccgag gcgacgacct gcacgctgcg cgcggcgacg tcgacgattt ggtcgggcgc
       61 atccgcttcg gtggtgggcg ctggaactga aacgtcacca cctgcggcga gcaggagcgc
      121 gtacggcgcg atgacggccg cgggtaccac cgacggcgat atccgtcggc ggcgagcgac
      181 actatgcctc cccatcacgt agcaccatac gagcaaacga gctttttttc acatccctca
      241 aagttccctt aagaagggcg acaagagcgc cggccagcta atagtcacga gaaggtaacg
      301 tttccgcagc tcatcggcgt gccaacggcg gacagcactg gttgtagacc taccgtggag
      361 agtgcgtcga accggtcgag tgctaaccga gccggcagta ttcactcgac cggttcgatg
      421 tcgtccttcg gtggacttcg tcggtcacgc cgtcggccgc cgcgccatac caagctgggc
      481 tcactggtca gcaagggctt cacaccgcgt caggccgccc ggctcaccga tctcgagttc
      541 aacgtgttct ttctgctgct gatcaacgcc ggcggggaca ccacccgcaa cctggtcgcg
      601 gccggcatcc tcgcgctgat ggaaaacccg gccgagcacg cccggctcgc ggcggacccg
      661 tcgctgctgc cgaacggcga ccaaggacac cgaactgcga gggattcgcg tgaaggccgg
      721 cgaccgggcg gcgatgttct atccgtcggc ccaccgcgac gagacacggt tggccgaccc
      781 cggcggcttc gacatcggtc gagcccgaac ccgcacctgg ctttcggcgg tggcggaacc
      841 atttctgcgt gcggtcgaat ctgatgaacg gcatccagtc gatgccggtc acgttcaggc
      901 cctgctgcgc cagcggaagc tgttgacgta cttgcccata tcgagggcga gaccgaggtt
      961 ggcacccgaa ggatggccgg agccgaactc cggcgtgaac tcgtgatacg gaccgatcgc
     1021 cgaagccacc aacgcgtagt cgttcttcac cgcgcacagt gcgaccagcc ggagccgggt
     1081 gtactcgccg ctggaatcct gcggatactg gtcgaacacg gcgccgtacc caggctcgta
     1141 accgaccatt gcgttcggga tttcgtagtc gatcgtggcg tcggggtagt actcggcgat
     1201 catctggtcg acgacctgct tcggcgtgcg ttttccggcg gcctcgcccc acagatccag
     1261 cgagccggta tcgccgccga gatattccag cgcgacccag tccgggtaca gcgtggcctt
     1321 gtaaaaagct ccgggcaccg ggtactgcac cgagaactcc ccgtcctcgg acacaaaccg
     1381 cgggttggtg tcgatcggtt taccgatcgg cggacgcccg caatcgggcg ggcaggtgta
     1441 tcgggggcgc ggcggcgtcg ccagagctga cgcggtcacc cccgaggcga ccaccacagc
     1501 gaggccggcg ctcaacgtcg cgaacagcca acgcaacgtc gtatggcgca gcggtgcaac
     1561 ctcatacagc ctggccgggg tggcatatcc cggccgcgcc gctgctgctt cggcgctgac
     1621 agcagtccga cgcgcggtcc gcgacgcgcg cgacgccgcc gctgcggaga ccccgcagtt
     1681 cgggcagaac gccatgtcgg gaaccacgtg gtcgcagtga gggcagagca cttgcgcctg
     1741 cggattcggc gggtcgtgtc gctcatgggt caagcagtac tgcaatgcaa ttcgcagacc
     1801 aagcagggcg acgacggtga tcaagaggtg agcaccgaca tgcaggccct ccagtgccgg
     1861 aatggcttcc gtgaggccga ggagggcgta caacgccagt gcacccagca cgctggacat
     1921 gatcgctttt cgtcggccaa accacaaagc cgcaccgacc gagccgccca ccgctgccgc
     1981 ggtcaacggc acggcaacgc cttgcacgac cgcttgggcc aacagcacgc tggcgggtcg
     2041 gtcaccggcg gtcaccccgg ttccgaactg cggtgccagc cgaatgagcg tggcagcggc
     2101 ggtgaagctg atggcgccca ccgacccgat tacgaagccg tccaatgact cccgggtggg
     2161 gcgagccaac cgcatcacca cggcgggcac cgtcatgagg agcgctcccc cgaccggtac
     2221 agcgatgcca acgatcagcg acaaccttct ggagtcagcg tctgccagcg acgcatcgta
     2281 gaagccggac accaatgagg cggtcgccaa cgcccaaccg acaccgaggc cgataccgag
     2341 cactgcgctc ggcaccagcg tacgaagcgt cacgtcgcgg tagacgtcgg cttcgtacag
     2401 gtaaagcacg aacacaagag gaagtccaag cgcgctgatc gcaatcaggg gtgcctgcca
     2461 ccgcagcagg gcgagggcca tcaacaacag aaagagaacc gcgaatgcca ctcgaaatgc
     2521 ggtgcgggag cggtgcggaa gatgcgggaa gagcgagctg gccacagaca ggcgcaccac
     2581 gtgttcgccg ggcgccgccg cgtaggcact cacccgaaag cggcccccgc cccgctccga
     2641 catctgcgca ccgcacgctc cgcaaaacgc cgcggccgac acctcggtac cgcaggaacc
     2701 gcacgagatg gtggccacgc cgtcatcgcc ggcgggcggt tgtgtcattc gggcgctacc
     2761 tgcgactgta tcttgagaag gttcctggcg aggttgtcag tgttgg
//
LOCUS       DASYLX010000809         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2131330,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000809 DASYLX010000000
VERSION     DASYLX010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131330"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGP27_18875"
     CDS             <1..444
                     /locus_tag="VGP27_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEV7853397.1"
                     /translation="VLNSRHKSTGEVFPQAIELMTTLNADASVAALAAGVDCATDVTG
                     FGLLGHLYKLARASGVTAVVDAAAVPYLDGARDALKAGYVSGGTRRNLDWVRPHADLS
                     AIDEDEALLLADAQTSGGLLIAGEIPGAPVIGELVPRGEHTILVR"
     gene            488..1030
                     /locus_tag="VGP27_18880"
     CDS             488..1030
                     /locus_tag="VGP27_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7853398.1"
                     /translation="MEAAQFTDISLRVDGSQHDVYVDNRITLLDLLREHLGVTAPKKG
                     CDHGQCGSCTVLLDGRRATTCLAFAVAHDGAEIVTAAGLGDADALHPVAAAFLEHDGF
                     QCGYCTPGQICSAVGMLDEVKAGQPSAVTDDIDGSPELTDDEIRERMSGNLCRCAAYP
                     NIVAAIYHSVSSSRPHQAAR"
     gene            1027..2019
                     /locus_tag="VGP27_18885"
     CDS             1027..2019
                     /locus_tag="VGP27_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV7853399.1"
                     /translation="MIPFDYHRATSVADAVTAVADRPDAVFLAGGTNLVDHMKLGLVE
                     PGLVVDVGHLPLSDVDQLPEGGLRVGADVRNSDLAAHPIVRSRYPVLARALLSGASGQ
                     LRNLATTAGNLLQRTRCVYFQDVTTPCNKRNPGTGCSAIGGYVRYHAVLGASENCVAV
                     HPSDMAVAMSALDADVLFVDGDGEHRIPLTEFHRLPGDRPEDDTVLPPGALITAVEIP
                     APPQGARSTYRKVRDRASYAFALTSVAAEVVVDDGTVSSARIALGGIAHKPWRARRLE
                     QRLVGSPATEDAFRAAADAELEQAEPLPGNEFKVELTRRTIVATLTSLTEGQRR"
     gene            2016..4109
                     /locus_tag="VGP27_18890"
     CDS             2016..4109
                     /locus_tag="VGP27_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7853400.1"
                     /translation="MTALEPRVIGTAVTRLDGPAKVTGTAPYAFEHRVDHPLYVHPVQ
                     ATIARGRVTAMYTTEAHAVDGVVDVVTVFDAPQLTDTSDGDLTILQDDEVHYRGQLIG
                     GVIAESAETARHAAGLVTVHYDSATHDSELRADHPKLYAPEQVNPSFPTDTSEGDPEA
                     ALRDAEVAVDQTYTTPHQYNNPMEPHACIAMWDSGGGRPRLTMYDSTQGVHVVRQRMA
                     TIFGLDKEQVRVIAKHVGGGFGSKGAPHSHNTLAVMAAQRAGGRPVKLALTRQQLFDV
                     AGYRTPTIQHIRLGAQRDGTLTAISHEVVEQTATVKEYAEQAGVPTRMMYAGANRKTS
                     HRLAPLDVPVPFWMRAPGECPGMYALEVAMDELAVACDIDPIELRLRNEPDVDPDTGK
                     PWSDRRLVECLHAGAERFGWAPRDPTPGKRRDGEWFVGTGVASAMYPAMIQPGNSARI
                     EHVGQGRYAVQIGAVDIGTGTWTALSQIAADALSCEMGAVELQIGDTELPVASVEGGS
                     SGISSWGRTIVAAARRFRQEHGETPAVGAATTVDAPENEEAKQHAMYSFGAHFVEARV
                     SRHTGEIRVSRMLGVFSIGRVINPTTLRSQLIGGMTMGLSMALHEEGFRDHRFGHVVT
                     RDLAGYHISAHADIPHIDAIALDDIDRHSNPMGSRGGGEIGIVGSAAAVVNAVYHATG
                     VRVRDLPVTCDAFLT"
     gene            4106..4882
                     /locus_tag="VGP27_18895"
     CDS             4106..4882
                     /locus_tag="VGP27_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV7853401.1"
                     /translation="MTDVADLVDEWGLRVDGDVVRASRAAVIPVRTSDGVRAVLKIGN
                     SEHEHLVLRRWAGDGAVRLLRADPHRGAVLLERLDSETLHTMSDVDGCEVVARLYSRL
                     HVAAMPQLPSLASILGQWADDFAALPRSAAIPHRLVEQAVTLCRALVKEPADHVLHGD
                     LHYGHVLAADREPWLAISPRPVNGDRHYELAPMLWHRWAEIGDNVRLGVQRRFYALVD
                     AAGFDEDRARSYVIVRVVREATRDAANLTRYIALAKAVQD"
BASE COUNT          810 a         1805 c         1642 g          730 t
ORIGIN      
        1 gtgctcaaca gcaggcacaa gtcgacgggc gaggtgttcc cgcaggcgat cgagctgatg
       61 acgacgctca acgcagacgc ttctgtcgcg gcgctggcgg cgggtgtcga ctgtgcgacg
      121 gacgtcaccg gcttcggcct gctgggccac ctgtacaagc tggcgcgcgc cagcggcgtc
      181 accgccgtcg tcgacgccgc ggcggtgccc tacctcgacg gcgcacgcga cgcgctgaag
      241 gccggttacg tcagtggcgg cacgcgccgc aacctcgact gggtccgacc gcatgccgac
      301 ctctcggcga tcgacgagga cgaggcgctg ctgctcgccg acgcacagac ctccggcgga
      361 ctgctgatcg ccggggagat ccccggcgcg cccgtcatcg gcgaactggt gccgcgcggc
      421 gaacacacga tcctggtgcg ctgacgccgg cccgtttcca ccccctgata ccgggtacgc
      481 cgcgcggatg gaggcagccc agttcaccga catcagcctg cgcgtcgacg gcagccagca
      541 cgacgtatac gtcgacaacc gcatcaccct gcttgacctg ctgcgcgaac acctcggcgt
      601 gaccgccccc aagaagggct gcgaccacgg ccaatgcgga tcctgcacgg tcctgctcga
      661 cggcagacgc gcgaccacgt gcctggcgtt cgcggtcgcc cacgacggcg ccgagatcgt
      721 caccgccgcg ggtctcggcg acgccgacgc cctgcacccg gtcgcggcgg ccttcctcga
      781 gcacgacggc ttccagtgcg gctactgcac gcccggccag atctgctcgg cggtcggcat
      841 gctcgacgag gtcaaggccg gccagcccag cgcggtcacc gacgacatcg acggctcccc
      901 cgagttgacc gacgacgaga tccgcgagcg catgagcggc aacctctgcc gttgcgccgc
      961 ctaccccaac atcgtcgccg ccatttatca ctccgtcagc tcctcgcgtc cgcaccaggc
     1021 cgcccgatga tccccttcga ctaccaccgc gccaccagcg tcgccgacgc cgtgaccgcc
     1081 gttgccgacc gcccagacgc cgtgttcctc gcgggcggca ccaacctcgt cgaccacatg
     1141 aaactcggcc tcgtcgagcc gggcctcgtg gtcgacgtcg gccacctgcc cttgtcggac
     1201 gtcgaccagt tgcccgaggg cggcctacgc gtcggcgccg acgtccgcaa cagcgacctc
     1261 gccgcgcatc cgatcgtccg cagccgctac ccggtgctgg cgcgcgccct gctgtccggc
     1321 gcgtcgggtc agctgcgcaa cctcgcgacc acggcgggca atctgctgca gcgcacccgc
     1381 tgcgtgtact tccaggacgt gaccacaccg tgcaacaaac gcaatcccgg cacgggctgc
     1441 tcggccatcg gcggctacgt ccgctatcac gccgtcctcg gcgcctccga gaactgcgtg
     1501 gctgtgcacc cgtccgacat ggctgtcgcc atgtcagcgc tggacgccga cgtgctcttc
     1561 gtcgacgggg acggtgaaca ccgcattccg ctgacagagt ttcatcggct gcccggcgac
     1621 cggcccgaag acgacaccgt gctgccgccc ggcgcgctca tcaccgccgt cgagatacct
     1681 gcgccaccgc agggtgcccg ctcgacgtac cgcaaggtcc gcgatcgcgc ctcgtatgcg
     1741 ttcgcgctga cgtccgtcgc cgccgaggtc gtcgtcgacg acggcaccgt ctcctcggcg
     1801 cgtatcgcgc tcggcggtat cgcccacaaa ccctggcgag cccgacggct cgagcagagg
     1861 ctcgtcggct cgccagccac cgaggacgcc tttcgcgcag ccgccgacgc cgagctcgag
     1921 caggcggaac cgttgcccgg caacgagttc aaggtcgaac tcacccggcg aaccatcgtc
     1981 gccaccctga cctcactgac cgaaggacag cggcgatgac cgccctcgag ccccgggtca
     2041 tcggcaccgc ggtcacccgc ctcgacgggc ccgccaaggt caccggcacc gcgccgtacg
     2101 cgttcgagca ccgcgtcgac catccgttgt atgtgcatcc ggtgcaggcg accatcgccc
     2161 gcggtcgagt caccgcgatg tacaccaccg aggcgcacgc cgtcgacggc gtcgtcgacg
     2221 tcgtcacggt cttcgacgca ccgcaactga ccgatacgtc cgacggcgac ctgacgatcc
     2281 tgcaggacga cgaggtgcac taccgcggcc agctgatcgg cggcgtcatc gccgagagcg
     2341 ccgaaaccgc caggcacgca gcgggtctcg ttaccgtgca ctacgacagc gcgacgcatg
     2401 acagcgagct gcgcgccgat cacccgaagt tgtatgcgcc cgaacaggtg aacccgtcgt
     2461 ttcccaccga cacttccgag ggcgaccccg aggcggcgct gcgcgacgcc gaggtcgccg
     2521 tcgaccagac ctacaccacg ccgcaccagt acaacaatcc gatggaaccc cacgcgtgca
     2581 tagcgatgtg ggactccggc ggcggccggc cccggttgac gatgtacgac tcgacccagg
     2641 gtgtgcatgt cgtccggcag cggatggcga cgatcttcgg gctcgacaag gaacaggtgc
     2701 gggtcatcgc caaacacgtc ggcggcgggt tcggctcgaa gggcgcgccg cattcccaca
     2761 acaccctggc cgtgatggcc gcgcagcggg caggcggccg cccggtcaaa ctggcgctga
     2821 cccgccagca gctgttcgac gtcgccggct atcgcacacc gaccatccag cacatccgcc
     2881 tcggcgcgca gcgcgacggc acgctgaccg cgatcagcca cgaggtcgtc gaacagaccg
     2941 cgacggtcaa ggagtacgcc gagcaggccg gggtgccgac ccgaatgatg tacgcgggcg
     3001 ccaatcgcaa gacgtcgcac cggctggcgc cgctcgatgt gcccgttcca ttctggatgc
     3061 gcgcacccgg cgagtgcccg gggatgtacg ccctcgaggt cgcgatggac gagctcgcgg
     3121 tggcctgcga catcgatccg atcgagctgc gcctccgcaa cgagcccgac gtcgacccgg
     3181 acaccggcaa gccatggtcg gaccgccgcc tcgtcgagtg cctgcatgcc ggcgccgagc
     3241 ggttcggttg ggcgccaaga gaccccacgc cggggaaacg gcgtgacggc gaatggtttg
     3301 tcggcaccgg tgtcgcgtcg gcgatgtacc cggcgatgat ccagcccggc aactcggctc
     3361 gcatcgagca cgtcggacag gggcgctacg ccgtgcagat cggcgccgtc gacatcggca
     3421 cgggcacatg gacggcgctg tcgcagatcg ccgccgacgc gctgtcgtgt gagatgggcg
     3481 cggtcgagct tcagatcggc gacaccgagc ttcccgtcgc gtcggtcgaa ggcgggtcct
     3541 ccggcatcag ttcgtggggc cgcaccatcg tcgctgcggc acgacgattc cggcaggaac
     3601 acggcgagac cccggcggtc ggcgccgcaa cgaccgtcga cgcgccggag aacgaagagg
     3661 ccaagcagca cgcaatgtac tcgttcggcg cgcatttcgt cgaggcgcga gtcagccgtc
     3721 ataccggtga gatccgtgtg tcgcgcatgc tcggcgtgtt ctcgatcggc cgggtgatca
     3781 atccgacgac actgcgctcg cagttgatcg gcggcatgac gatgggactg tcaatggcgt
     3841 tgcacgagga gggctttcgc gaccaccgct tcggccatgt cgtgacgcgg gacctggctg
     3901 gatatcacat cagcgcgcat gccgacatcc cacacatcga cgccatcgcg ctcgatgaca
     3961 tcgacaggca ctccaacccg atgggctcgc gcggcggggg cgagatcggc atcgtcgggt
     4021 ccgcggcggc ggtggtcaac gccgtctatc acgccaccgg cgtgcgggtg cgagacctac
     4081 cggtgacgtg tgacgccttt ctgacgtgac cgacgtcgcc gacctcgtcg acgagtgggg
     4141 tcttcgcgtc gacggcgacg tggtgcgcgc ctcccgggcc gcggtcatcc ccgtccgcac
     4201 atccgatggg gttcgcgcgg tgctgaagat cggcaactcc gagcacgaac acctggtgct
     4261 gcggcgctgg gccggcgacg gcgcggtgcg gcttctgcgc gccgatccgc accgaggggc
     4321 tgtactgctg gagcgtctgg attcagagac gctgcacacc atgtccgacg tcgacggctg
     4381 cgaggtggtc gcacggttgt acagtcgcct tcatgtggcg gcgatgccgc aattaccctc
     4441 gctcgcatcg attctcggac aatgggccga cgactttgcg gcgctgccac ggagcgccgc
     4501 gataccgcac cggctcgtcg agcaggcggt cacactctgc cgcgcattgg tgaaagagcc
     4561 ggccgaccat gtgctgcatg gcgacctgca ctacgggcac gtcctggctg ccgaccgcga
     4621 gccttggctg gcgatctctc ccaggccggt gaacggcgac cggcactacg agctggcgcc
     4681 gatgctgtgg caccgctggg ccgagatcgg cgacaacgtc cgcttaggag ttcagcggcg
     4741 gttctatgcg ctggtcgatg ccgccggctt cgacgaggac cgcgcacgtt cgtatgtcat
     4801 cgtccgggtg gttcgggagg cgacccgcga cgccgccaac ttgacgaggt acatagcgtt
     4861 ggcgaaggcc gtgcaggact gaccaagtca catccgcgag taccgtgtca gcgccaagct
     4921 gtagaggccg tatgccgcga aacctactgc ggcggcgatc aatatcgccc cgccgagccg
     4981 agtagat
//
LOCUS       DASYLX010000810         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_44951, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000810 DASYLX010000000
VERSION     DASYLX010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44951"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGP27_18900"
     CDS             <1..576
                     /locus_tag="VGP27_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853402.1"
                     /translation="VGLEKADSGAVRVGETVKLSYVDQGRSNLDPEKSVWETVSDGLD
                     YINVGQTEMPSRAYIGAFGFKGPDQQKKAGVLSGGERNRLNLALTLKEGGNVILLDEP
                     TNDLDVETLGSLENALLEFPGCAVVISHDRWFLDRVATHILAWEGDDENSAKWYWFEG
                     NFEGYEKNKIERLGPEAARPHSVTYRKLTRD"
     gene            complement(637..1446)
                     /locus_tag="VGP27_18905"
     CDS             complement(637..1446)
                     /locus_tag="VGP27_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006769569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7853403.1"
                     /translation="MPFLGITVIIVYCLAPFYWMVVSSLRRPNDQFSNSLLPNPLSTE
                     NYTAALDPNNGFPRSILNSLIVAGTTTIVGLVIAIFTAYALARLDFRFKNLALAIIIA
                     TSMFPGISMIVPLFKLFVQIGWINGYQAMIVPSLSFALPLAVWNLTAFFRQMPYELEE
                     AAQIDGCTPGQAFRKVILPLAAPGVFTTAIITFIAAWNEYIIALSMVNDPARKTAPVI
                     VSQFAGSSGSGFDQPFGTQMAAGVIVTIPLVILVLLFQRRIVTGLTAGGVK"
BASE COUNT          280 a          498 c          556 g          222 t
ORIGIN      
        1 gtcggcctcg agaaggcgga cagcggcgcg gtcagggtcg gcgagacggt caagctgtcc
       61 tacgtcgacc agggccgcag caacctcgat ccggagaaga gcgtctggga gaccgtctcc
      121 gacggcctgg actacatcaa cgtggggcag acggagatgc cgtcgcgcgc ctacatcgga
      181 gccttcggct tcaagggccc cgaccagcag aagaaggccg gcgtgctgtc cggcggtgag
      241 cgcaaccgcc tgaacctggc gctgaccctc aaggagggcg gcaacgtcat cctgctggac
      301 gagccgacca acgacctcga cgtcgagacc ctcgggtcgc tggagaacgc gctgctcgag
      361 ttccccggct gcgccgttgt gatctcccac gaccgctggt tcctcgaccg agtcgccacc
      421 cacatcctcg cgtgggaggg cgacgacgag aactcggcga agtggtactg gttcgagggc
      481 aacttcgagg ggtacgagaa gaacaagatc gagcggctcg ggccggaggc ggcgcggccg
      541 cactcggtca cgtaccgcaa gctcacgcgc gactgatctg ccgacgacag caagcggccc
      601 ccggggaaac cccgggggcc gcttgctgtg caggggttac ttgacgccac ccgccgtcag
      661 gccggtgacg atccgccgct ggaacagcag gacgaggatg accagcggga tggtgacgat
      721 cacgccggcg gccatctggg tgccgaacgg ctggtcgaag cccgaacccg agctcccggc
      781 gaactggctg acgatgaccg gcgcggtctt gcgggccggg tcgttgacca tggacagcgc
      841 gatgatgtac tcgttccacg ccgcgatgaa ggtgatgatc gccgtggtga agacgccggg
      901 cgccgccagg ggcaggatca ccttgcggaa cgcctgcccc ggcgtgcagc cgtcgatctg
      961 cgcggcctct tccagctcgt acggcatctg ccggaagaag gcggtgaggt tccagaccgc
     1021 gagcggcagc gcgaaggaca ggctgggcac gatcatcgcc tggtacccgt tgatccagcc
     1081 gatctgcacg aacagcttga acagcggcac gatcatcgag atcccgggga acatcgacgt
     1141 ggcgatgatg atggcgagcg ccaggttctt gaaccggaag tccagccgcg ccagcgcgta
     1201 cgccgtgaag atggcgatca ccaggcccac gatcgtcgtg gtccccgcga cgatgaggct
     1261 gttcaggatg ctgcggggga acccgttgtt cgggtccagc gccgccgtgt agttctcggt
     1321 cgacagcggg ttcggcagca gggagttgga gaactggtcg ttgggccgcc gcaggctgga
     1381 gaccaccatc cagtagaacg gcgccaggca gtacacgatg atgaccgtga tcccgaggaa
     1441 cggcagccag cgccgccacc cggtgaccgt gttcgcactc ggcggcttct ggtcgcgccg
     1501 caggcggcgg gtcgcgtccc gttccgcctc ggcagcgagg gggccggcga cggccg
//
LOCUS       DASYLX010000811        11109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_814062,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000811 DASYLX010000000
VERSION     DASYLX010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814062"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGP27_18910"
     CDS             <1..219
                     /locus_tag="VGP27_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV7853404.1"
                     /translation="FARVLLAKPKAVFLDEATSALDEGQEFALYRALRTQLPDCIVVS
                     VSHSPTLDQHHDRRLELLGDGAWRLQPV"
     gene            complement(216..1703)
                     /locus_tag="VGP27_18915"
     CDS             complement(216..1703)
                     /locus_tag="VGP27_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV7853405.1"
                     /translation="MKQIPDIVAAAAVSSWSDDVDVVVIGFGIAGGCAAVSAAAAGAK
                     VLVLEKAAAAGGTSAMAGGHFYLGGGTAVQQATGHDDSADEMYSYLVAVSPDPDHEKI
                     RAYCDGSVEHFDWLEDLGFQFERSFYPGKIVVPPGTEGLSYTGNEKVYPFTEKAKPAP
                     RGHSVPVPGELGGAAMVIDLLVKRADELGVEIRYETGATNLVAEQGSPEDRVVGVRWK
                     HFTETGAVKAKAVIIAAGGFAMNPEMVAEHTPALGQKRRTKHHGVVEPYILGNPNDDG
                     LGIRMGVSAGGVAKNLDELFITAAAYPPEILLTGIIVNKDGKRFVTEDSYHSRTSAFV
                     LEQPDQIAYLIVDEDHMRMPEMPLIRFIDGWETVEEMAAALRIPEGNLVETLDRYNEH
                     AAQGEDPDFHKQPDYVAPQTKGPWAAFDMSLGRAMYSGFTMGGLAVTIDGQALRQDGS
                     VIPGLYAAGACASNIAQDGKGYSSGTQLGEGSFFGRRAGTHAARS"
     gene            complement(1732..2202)
                     /locus_tag="VGP27_18920"
     CDS             complement(1732..2202)
                     /locus_tag="VGP27_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7853406.1"
                     /translation="MHDQTPVQIAWVTKDLDATENALTSLLGAKKWIRVPDVHFGPET
                     CTYRGRPADFVAHISFSYAGDTQLELIAPVSGESVYSEFLDRAGAGLHHICVEVDDVD
                     EAVSGAEVVQRGVMPGGMEFAYVTAEAAGVPYIEIAHITPEIRAFFDYVKQEQQ"
     gene            complement(2248..2877)
                     /locus_tag="VGP27_18925"
     CDS             complement(2248..2877)
                     /locus_tag="VGP27_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7853407.1"
                     /translation="MIDVDYQVNAVRRTVGDRTLDAGEARVVTISQAYGTDADDLWDA
                     CTNIDRIPRWFLPITGDLRAGGRYELEGNANGTILTCDPPREFTATWECGEDVSWIEV
                     RICSEGSHRSRVELQHISHVDEHWEQFGPGATGMGYDGALVGLTVHLTTGGAVDPAAG
                     QEWMASEDGRRFMKLSGEAWYDASIAAGADRDWARAAADRCVAAYLGEN"
     gene            complement(2874..3212)
                     /locus_tag="VGP27_18930"
     CDS             complement(2874..3212)
                     /locus_tag="VGP27_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7853408.1"
                     /translation="MHVFDVLGDPVRRRILELLADGELTAGAIGADIQAEFEISQPAV
                     SQHLKVLRDNGFTSVRPDGQRRLYAVSGEALREADAWLAGFRRFWTPHLDALATEIAR
                     GKRQRRRSTT"
     gene            complement(3262..3957)
                     /locus_tag="VGP27_18935"
     CDS             complement(3262..3957)
                     /locus_tag="VGP27_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853409.1"
                     /translation="MILRRHWLRSLAAAAAASILALTIPALPAHATPGVLVYPGMEIH
                     QGTSVCTLGFVDPGARTAFTAGHCRGEGGVVDRDGNPIGTLTSFRDNTPDGATVATDQ
                     MIADWEAINLAPDVTVNDILPGGRVLVSDPTVVPTPGLGVCHFGVVTGESCGNIEAVN
                     NGWFTMANGVVSQKGDSGGPVYVVTPDGRAAIVGMFNSTWGNYPAAVSWQTASQQAHQ
                     DVVQSASGSAEPR"
     gene            complement(4077..4718)
                     /locus_tag="VGP27_18940"
     CDS             complement(4077..4718)
                     /locus_tag="VGP27_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV7853410.1"
                     /translation="MTDRLAKGEKLERGQSLTSRNGAYTLTLQDDGNLVLAAQGHAVW
                     ATGTNGQDAEHLDVQEDGNVALYTSDKPIWHTDTKGAKDIRLILQDDRNLVVYGFDGV
                     AWASGTNTDEAPPPPPGAGQAAPDVAPATAEEPLPPAAAEEPLPPAEAPAPPPPPPPP
                     PPAPRTYTVVSGDTLWAISERFYGDGSKYQRVADASGVSNPDLIHPGQVLTIP"
     gene            complement(4836..6242)
                     /locus_tag="VGP27_18945"
     CDS             complement(4836..6242)
                     /locus_tag="VGP27_18945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853411.1"
                     /translation="MQTVFDAHVDPDLVERSLAASAFHSMWLDMPRPSYGALSGSVTA
                     DLLVVGAGYTGLWTALHAAQRNPDQKIVLVDAERVGWAASGRNGGFVDASLTHGLANG
                     KARWPNEIEKLEAMGRDNLDGMQADIARLGLDAEWQRTGMLTVATEPHQVDWLSEAAD
                     DGEGRFLTQGEVRSEVSSPTYLAGLFSPESTAIVHPAKLAFELARACAEAGVRIYERT
                     NVTSMECAGAAIRFAAGNATITAGRAVLATNVFPSLIMRNRLRTVPVYDYVLATEPLT
                     DAQLDRIGWTNRQGIGDCANQFHYYRRSADNRIVWGGYDAVYHFGRRVKPAYEDRSAS
                     YRRLAAHFFITFPQLDDVRFSHRWAGAIDTSTRFCAHWGLAHAGRVAYVNGFTGLGVG
                     AARFAADVCLDLLDGRPTERTELEMVRKRPLPFPPEPLASIGIQATRWSLDRADHFAG
                     RRNVLLRTLDALGLGFDS"
     gene            complement(6325..7254)
                     /locus_tag="VGP27_18950"
     CDS             complement(6325..7254)
                     /locus_tag="VGP27_18950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV7853412.1"
                     /translation="MDWSKAWEDVLPALMHDPVTFAVPFFLILLIIEWAAARKLAHIE
                     DDRSPSGTYLRPDAWASIWMGVVSVGTSAVMNGIALLGYAALYVYVAPWQLPSNAWYT
                     WVIAILGVDLTYYWYHRMAHRVRLFWATHQAHHSSQYFNLATALRQKWNISGDVFLRA
                     LLPMFGVPPWMVFASFSINLIYQYWIHTERIGKLWRPIEFVFNTPSHHRVHHGMDEVY
                     LDKNYGGILIIWDRLFRSFQPEDFRPHYGLTKQVDTYNIWKLQTHEYVAIARDVRGAG
                     RWRDRLGFVFGPPGWEPPQAVQPDIPAPLTVSK"
     gene            complement(7296..8843)
                     /locus_tag="VGP27_18955"
     CDS             complement(7296..8843)
                     /locus_tag="VGP27_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV7853413.1"
                     /translation="MARDNTLVETVNGALRGIDDGRVTAWKGVRYAAPPVGDLRWRAP
                     QPVTPSTTVIDATRYGVVCPQPIDPRIPIDLGAPQGEDCLTLNVWASSDTTAGDRKPV
                     MVWVHGGAYILGSASQPLYDGRVLAGDGEVVVVTMNYRLGALGFLDLSEFDSPRRRYD
                     SNLGLRDVLAALQWVRDNIATFGGDPDRVTVFGESAGAGIITTLLTIPAAEGLFAGAI
                     AQSSPATSVYDISRARGITRQVMNRLGLSASDVERLPDVPVGAIVAASTDVFYETPVN
                     NPGTLAFAPNVDGDLVPDYPVKLAREGRSHPVPLIIGTNKHEAALFRWMKSPLMPITP
                     EAISAMFTEIAAEQPSLNLPTDYHTGAAYAGLRGKARGMGVARDLGFRMPSVWLAEGH
                     SAVAPVYLYRFDFATPMLRLLRIAAAHATELPFVWGNLESGPKDPTFKLGGIKAGRVV
                     SERVRARWLNFATQAKPAGLPGEPEWLPYQEPDRACLLIGREDTVVNDIDTDIRATWG
                     AQVLNFR"
     gene            8949..9857
                     /locus_tag="VGP27_18960"
     CDS             8949..9857
                     /locus_tag="VGP27_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV7853414.1"
                     /translation="MSALRRWIVDDFEKGAGRHDDPKIYGGPPGDPGLIGPGSMSWEI
                     NADLASVSQAGLPAIVLEVLHPSVIAGVQNLSDYRADPFQRARATLGYVLATTFGNTE
                     AAIAIIERVKHIHSFVNGTRPDGVPYRALDPELIVWVHTCIPWMILRVFEETKRPLSQ
                     HEKDRYLSEQAVIGRMSGADWVPETAAQLDDYVDSMRPRLSVNAQTREFVDFLMTSPF
                     FPDLPERLDRQLHRFILYAGMSRAPRWAQELIGYDRPPIATRALTGPMLRLDAQRLRW
                     AFGAPRYLQLAQQRAAGARQGKVAAS"
     gene            9857..10477
                     /locus_tag="VGP27_18965"
     CDS             9857..10477
                     /locus_tag="VGP27_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853415.1"
                     /translation="MDARLIEVVGGASVAGRIDEAMDRTSQRILDAALSEAAAVGLQR
                     ITVEDVVRRADVSRMTAYRRYPRRDDLVAALVRRETQRFLGAVADAIEGVEDSDDGVA
                     EAFIAAVSFAREHPILRRAAQNEPAPIADSVELLTMGSAFIADYIHGDATGAPSRQVR
                     WVADVFARLFVTYISMPPSDPDFTDDAELRRFAHEVLTPMVQQAVR"
     gene            10487..10825
                     /locus_tag="VGP27_18970"
     CDS             10487..10825
                     /locus_tag="VGP27_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEV7853416.1"
                     /translation="MRKWVLLGAAIAVEVAATLSLRASQDHSAWLIVVVAGYTGAFVL
                     LTLVLRAGLSIGVAYGIWGALGTVGTAVLAAAIFGDPLTWPIAVGIGLIIAGVLLVEL
                     GARHTEEAAA"
     gene            10825..>11109
                     /locus_tag="VGP27_18975"
     CDS             10825..>11109
                     /locus_tag="VGP27_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEV7853417.1"
                     /translation="MWATLTGAILFEVVATVALRASEGFRRRIWIVPVTIGYLVSFYL
                     LWLTLRLGMHVGIAYGVWTACGVAVVAVAAKVLFDEPLTWLMGLGIALIVG"
BASE COUNT         1753 a         3654 c         3735 g         1967 t
ORIGIN      
        1 ttcgcccgtg tgctgctggc gaaaccgaaa gcggtgttcc tcgacgaggc gacgtccgcg
       61 ctcgacgagg gtcaggagtt cgcgctctac cgagcgctac gcacgcagct gccggactgc
      121 atcgtggtca gcgtcagcca cagccccacg ctcgaccagc atcatgaccg gaggctggaa
      181 ctgctcggcg acggcgcctg gcggctgcag cccgtctaac tcctggccgc gtgcgttccc
      241 gcgcggcgcc cgaagaacga cccctcgccc aactgcgtgc cgctggagta gcccttgccg
      301 tcctgggcga tgttggacgc gcaggcgccc gcggcataca ggccgggaat cacactgccg
      361 tcctgccgca gggcctgacc gtcgattgtc accgccagcc cacccatggt gaacccggaa
      421 tacatcgcgc gacccagcga catgtcgaag gccgcccacg gtcccttggt ctgcggcgca
      481 acataatccg gctgcttgtg gaagtcggga tcctccccct gtgccgcgtg ctcgttgtag
      541 cggtccaggg tctcgaccag attgccttcg ggaatgcgca gcgcggccgc catctcctca
      601 accgtctccc agccgtcgat gaaccggatc agcggcatct ccggcatccg catgtgatcc
      661 tcgtcgacga tcaggtacgc gatctggtcg ggctgctcga gaacgaacgc ggaggtgcgc
      721 gagtggtacg agtcttcggt cacgaatcgc ttgccgtcct tgttgacgat gatcccggtc
      781 agcaggatct ccggcggata ggcggcggcg gtgatgaaca gctcgtccag gttcttcgcg
      841 actccgcccg ccgacacgcc cattcgaatg ccgaggccgt cgtcgttggg gttgcccagg
      901 atgtagggct ccaccacgcc gtggtgtttc gtgcgtcgct tctggcccag cgcaggtgtg
      961 tgctcggcga ccatctcggg gttcatcgcg aacccgcccg cagcgatgat gaccgccttg
     1021 gccttgaccg caccggtctc ggtgaagtgt ttccatcgca cgccgacgac ccggtcttct
     1081 ggcgaaccct gttcggcgac caggtttgtc gcgcctgtct cgtaccggat ctccacgccg
     1141 agctcatcgg cgcgtttcac caggaggtcg atgaccatcg ccgcaccgcc cagctcaccg
     1201 ggcaccggga cggaatggcc gcgcggcgcg ggcttcgcct tctcggtgaa cggatacacc
     1261 ttctcgttgc cggtgtagga cagcccctcg gtgccgggcg ggacgacgat cttcccggga
     1321 tagaaactgc gctcgaactg aaaccccagg tcttccaacc agtcgaagtg ttcgacgctg
     1381 ccgtcgcagt aagcgcggat tttctcgtgg tctggatccg gcgataccgc gacgaggtag
     1441 gagtacatct cgtcggcgct gtcgtcgtgg ccggtggcct gctgcaccgc ggtgccgccg
     1501 ccgagataga agtgcccgcc tgccatcgca ctggtccccc cggcagcggc ggccttctcc
     1561 agcaccagca ccttggcgcc tgcggccgcc gcgctcacag ccgcgcagcc gcccgcgatt
     1621 ccgaatccga tgacgacgac gtcgacgtcg tcggaccagc tcgacaccgc ggcggcggcg
     1681 acgatgtccg ggatctgctt catcgatttg tcctcgcgca agcgctgaac ctcactgttg
     1741 ctcctgtttg acgtagtcga aaaacgctcg gatctccggc gtgatgtgtg cgatctcgat
     1801 ataaggcacg cctgccgcct ccgcggtgac gtacgcgaac tccattccgc cgggcatgac
     1861 tccgcgttgt accacctcgg ccccgctgac cgcctcgtcg acgtcgtcga cctcgacgca
     1921 gatgtggtga agcccggcgc ccgctcgatc gaggaactcg gagtagacgc tctcgccgct
     1981 gaccggcgcg atcaattcga gctgagtgtc cccggcatag ctgaacgaga tgtgggcgac
     2041 gaagtccgcc ggccggccgc ggtaggtgca cgtctcgggg ccgaagtgca catccggcac
     2101 acggatccac ttcttggcgc ccaacagcga ggtcagggcg ttctcggtgg cgtcgaggtc
     2161 cttcgtcacc caggcgatct ggacaggtgt ctggtcatgc atcgcatcga gaatatctcc
     2221 ggtaggcccg gctggctaga acgtgttcta gttctccccc agatacgcgg cgacacaccg
     2281 atccgcggcc gctcgtgccc agtcccgatc tgcacccgcc gcgatgctgg cgtcgtacca
     2341 cgcctcgccg gacagcttca tgaatcgccg gccgtcctcg gaggccatcc actcctggcc
     2401 ggctgccggg tcgacggccc cgccagtcgt cagatggaca gtgagcccga ccagcgcgcc
     2461 gtcgtagccc atgccggtgg cgccgggacc gaactgttcc cagtgctcgt cgacgtgcga
     2521 gatgtgctgg agttcaacac gtgaccgatg cgaaccttca ctgcagatcc gtacctcgat
     2581 ccagctgacg tcctcgccgc actcccatgt cgcggtgaac tcgcgcggcg gatcgcaggt
     2641 caggatcgtg ccgttggcgt tgccctcgag ctcgtagcgg ccacccgcgc gcaggtcgcc
     2701 ggtgatcggc aggaaccacc gggggatgcg gtcgatgttg gtacaggcgt cccagagatc
     2761 gtccgcatcg gtgccgtagg cctggctgat cgtgacgacg cgggcctccc ccgcgtccaa
     2821 tgtccggtcg ccgacggtgc gccgcacggc gttgacctgg tagtcgacgt caatcatgtt
     2881 gtgctccttc gtcgttgtcg cttgccgcgc gcgatttcgg ttgcgagcgc atcgaggtgt
     2941 ggagtccaga agcgacggaa cccggcgagc cacgcgtcgg cttcccgaag cgcctcgccg
     3001 ctgacggcgt acagccgtcg ctgaccgtcc ggtcgtaccg aggtgaatcc gttgtcgcgc
     3061 aacaccttca aatgttgtga cactgcaggc tggctgatct cgaactccgc ctgaatgtcg
     3121 gcaccgatgg cgccggccgt cagttcgccg tcggcgagca gttcgaggat gcgcctacgc
     3181 accggatcgc cgaggacgtc gaacacgtgc accagccgac tttaaaagca ggcacttata
     3241 taagtcaagc cttaaggagg atcaccgagg ctctgcgctg ccggatgcgg actgcaccac
     3301 atcctggtgc gcctgctggc tggcggtctg ccaggacacc gccgctggat agttgcccca
     3361 tgtgctgttg aacatcccga cgatcgcggc ccgcccgtcg ggggtgacca cgtacaccgg
     3421 ccctccggag tcgccctttt ggctcactac accgttcgcc atcgtgaacc agccgttgtt
     3481 gaccgcttcg atgttgccgc aactctcgcc ggtgaccact ccgaagtgac agaccccgag
     3541 gccgggtgtc ggcacgacgg tcgggtcgga gaccaggacg cggccgccgg gcaggatgtc
     3601 gttcacagtg acatccggtg cgaggttgat ggcctcccag tcggcgatca tctggtcggt
     3661 cgccacggtc gccccgtccg gagtgttgtc gcggaacgac gtcagggtgc cgatgggatt
     3721 gccgtccctg tccaccacac ctccttcgcc gcggcagtga cccgcagtga acgccgtgcg
     3781 ggcacccggg tcgacgaacc cgagggtgca cacgctcgtg ccctggtgga tctccatgcc
     3841 cggatagacc aacacgccgg gagtggcatg tgcgggcaag gcggggatgg tcagcgcaag
     3901 aatcgacgcg gccgccgcag cggccagcga gcgcaaccaa tgtcggcgca agatcatctc
     3961 cgattggtag gggtcagtgg gctcctaccc taccggcgcg ggtgcgcaca gcaagccagc
     4021 ccggaccgtt cggcccgggc tggctttcgt cacatctgct cttttggtca cttctgctac
     4081 ggaatcgtca gcacctgccc cggatgaatc aggtcggggt tgctgacgcc actggcgtcg
     4141 gcgacccgct ggtacttgct gccgtcgccg tagaagcgtt ccgagatggc ccacagcgtg
     4201 tccccggaga ccacggtgta ggtgcgcgga gccggcggtg gtggcggtgg tggtggcggc
     4261 ggggccgggg cctcggcggg cggcaacggt tcctcggcgg ccgcgggcgg caacggttcc
     4321 tcggcggtgg cgggcgcaac gtcgggggct gcttgccctg cgcccggagg cggcggtgga
     4381 gcttcgtcgg tgttcgtgcc cgacgcccat gccaccccgt cgaatccgta caccaccagg
     4441 ttgcggtcgt cctgcaggat cagccgaata tccttggcgc ccttggtgtc ggtatgccag
     4501 atcggtttat cggacgtgta gagcgcgacg ttgccgtcct cctgcacatc gaggtgttcg
     4561 gcatcctggc cgttggtgcc cgtcgcccat acggcgtggc cctgcgcggc gagcaccagg
     4621 ttgccgtcgt cctgcagggt cagcgtgtag gccccgttcc gcgaggtcag cgactggcca
     4681 cgctcgagct tctcaccttt ggcgagcctg tccgtcacgt catcgaccct tcgttttccc
     4741 ggtaaccaac gcgggtgagc ttactgatct tggtaggcca tgtggccggt tcggaccggc
     4801 ccaatcgaaa cgtcgtgcaa acgctgcgtg gacattcagg aatcgaagcc gagcccgagg
     4861 gcatcgagcg tgcgcagcag gacattgcgg cgccccgcaa agtggtcggc gcggtcgagc
     4921 gaccatctcg tcgcctggat tccgatgctg gccaaaggtt caggcggaaa cggcaagggt
     4981 cgcttgcgca ccatttccaa ctcggtgcgc tcggtcggtc gaccgtcgag caggtcaaga
     5041 cagacgtcgg cggcgaaccg cgccgcgccg acgccgagcc cggtgaagcc gttgacgtac
     5101 gccacccgtc cggcgtgcgc cagcccccag tgcgcgcaga accgggtgct ggtgtcgatc
     5161 gctccggccc agcgatggct gaatcggacg tcgtcgagtt gggggaaggt gatgaagaaa
     5221 tgcgctgcga gccgacgata gctcgcggac cggtcctcgt aggccggctt gaccctgcgc
     5281 ccgaagtggt agacggcgtc atagccgccc cacacgattc ggttgtcagc gctgcgccgg
     5341 tagtagtgaa actgattggc gcagtcgccg attccctggc ggttcgtcca tccgatgcgg
     5401 tcgagctgag catcggtgag cggctcggtg gccagtacat agtcgtacac cggcaccgtc
     5461 cgtagccgat tgcgcatgat caggctcggg aagacgttcg tcgccagcac cgcccgccct
     5521 gcggtgatcg ttgcgttgcc cgccgcgaac cggatggccg cgcctgcgca ttccatcgat
     5581 gtgacgttcg tccgctcgta gatgcggaca cccgcctccg cgcatgcccg agccagttcg
     5641 aatgccagct tggcgggatg gacgatcgct gtgctctcgg ggctgaacag tcctgcaagg
     5701 taggtcggcg acgacacctc gctgcgcact tcgccctgcg tcaggaaccg gccctcgccg
     5761 tcgtcggcgg cctcgctcag ccagtccacc tggtgcggtt ccgtcgcgac cgtcaacatc
     5821 ccggttcgct gccattcggc gtcgagccca agccgcgcaa tatcggcctg catgccgtcg
     5881 aggttgtcgc gacccatcgc ctcgagcttc tcgatctcgt tgggccagcg tgcttttcca
     5941 ttcgccagac cgtgggtcag gctggcgtcg acgaagccgc cgttgcgacc tgacgccgcc
     6001 cagcccacgc gttcggcgtc gacgaggacg atcttctggt ccggattgcg ctgtgcggca
     6061 tgcaatgccg tccacagccc ggtgtatccg gcgcccacca ccaacaggtc ggccgtcacg
     6121 gaaccggaca gcgcgccgta ggacggccga ggcatgtcca gccacatcga gtggaacgcg
     6181 cttgccgcca gcgaccgttc gacgaggtct gggtcgacgt gggcatcgaa gaccgtctgc
     6241 acaccgcgac actacggtgt ggggcaccac cggcggcgct gattcagatg cgggcgccgt
     6301 gatcagcgat gggcgcaggc agcgctactt cgacacggtg agcggcgcgg ggatgtcggg
     6361 ctgcacggcc tggggcggct cccagcccgg agggccgaaa acgaagccaa ggcggtcacg
     6421 ccatcggccg gcgccgcgga cgtcgcgcgc gatcgcgacg tattcgtggg tctgcaactt
     6481 ccagatgttg taggtgtcca cctgtttggt aaggccgtag tgcggccgaa aatcctcggg
     6541 ctggaagctg cggaacagtc gatcccagat gatcaggatg ccgccgtagt tcttgtcgag
     6601 gtagacctcg tccatgccgt ggtgcacccg gtggtgcgac ggcgtgttga agacgaattc
     6661 gattggccgc cagagcttgc cgatacgctc ggtgtgaatc caatactggt agatcaggtt
     6721 gatggagaag ctcgcgaaca ccatccacgg cggtacgccg aacatcggca gcagcgcgcg
     6781 aaggaaaacg tcgccgctga tgttccactt ctgacgcagc gcggtggcga ggttgaagta
     6841 ctggctggag tggtgcgcct gatgcgtcgc ccagaacagg cggacgcggt gcgccatccg
     6901 gtgataccag taataggtga ggtcgacgcc gaggatcgcg atcacccagg tgtaccaggc
     6961 gtttgaggga agctgccagg gcgcgacgta gacgtagagt gccgcgtagc cgaggagcgc
     7021 tatcccgttc atcaccgcgc tggtgccgac cgacacgacg cccatccaga tgctggccca
     7081 ggcgtccggc ctgaggtagg tccccgacgg cgaacgatcg tcttcgatat gggcgagttt
     7141 gcgtgcggct gcccattcga tgatgagcag gatcagaaag aacgggacgg cgaacgtgac
     7201 cgggtcgtgc atcagtgccg gaagtacgtc ctcccaagcc tttgaccagt ccactcgcga
     7261 cacctcccgg gaaagtctag ccagcccggt ggcggttacc tgaagttgag tacctgggcg
     7321 ccccaggtgg cccggatgtc ggtgtcgatg tcgttgacga cggtgtcctc cctaccgatg
     7381 agcaggcacg cccggtcggg ctcctggtag ggcagccact ccggctcgcc gggcagtcct
     7441 gcgggtttgg cctgtgtcgc gaaattcagc cagcgggccc tcacccgttc cgagaccact
     7501 cgaccggcct ttatcccgcc cagcttgaag gtcggatcct tgggtccgga ctccagattg
     7561 ccccatacga aaggcaattc ggtggcgtgg gcggcggcga tacgtagtag ccgcagcatc
     7621 ggcgtcgcga agtcgaagcg gtaaaggtac acgggtgcca cggcgctgtg cccttcggcg
     7681 agccataccg acggcatgcg gaacccgagg tcacgcgcga cgcccatgcc gcgagccttg
     7741 ccgcgcaggc cggcatatgc ggcaccggtg tggtagtcgg tcggcagatt caggctcggt
     7801 tgttcggcgg cgatctcggt gaacatcgcg cttatcgcct cgggggtgat cggcatcagc
     7861 ggcgacttca tccacctgaa cagcgccgcc tcgtgtttgt tggtgccgat gatcagcgga
     7921 accgggtgag accggccctc ccttgcgagc ttcaccgggt agtcgggcac cagatcgccg
     7981 tcgacgttcg gggcgaatgc cagcgtgccg gggttgttca ccggcgtttc gtagaagacg
     8041 tcggtggacg ccgcgacgat ggcgccgacc ggaacgtccg gcaaccgctc gacgtcagac
     8101 gccgaaagtc cgaggcggtt catcacctgg cgcgtaatgc cgcgcgcacg gctgatgtcg
     8161 tagaccgacg ttgccggcga gctctgggcg atggcgcccg cgaacagccc ttcggccgcc
     8221 gggatggtga gcagcgtggt gatgatgccc gcgcccgccg attcgccgaa cactgtgacg
     8281 cggtccgggt cgccgccgaa cgtggcgata ttgtcgcgca cccactgcag cgctgcgagc
     8341 acgtcgcgca gaccgaggtt ggagtcgtag cggcgccgag ggctgtcgaa ctcggacaga
     8401 tcgaggaagc ccaacgcccc gagccgatag ttcatcgtga cgacgaccac ctcgccgtca
     8461 ccggccagta cgcggccgtc gtagagcggc tggctcgccg aacccaggat gtaggcgccg
     8521 ccgtgtaccc agaccatcac cggcttgcgg tcgcccgcag tcgtgtcgga cgacgcccag
     8581 acgttgagcg tgaggcagtc ctcgccctga ggcgcgccca gatcgatggg aatccgcggg
     8641 tcgatcggct ggggacagac gacgccgtag cgagtggcat cgatgacggt cgtcgacggt
     8701 gtcaccggct gcggggcacg ccacctcagg tcgccgaccg gcggggcggc gtagcgcaca
     8761 cccttccacg ccgtcacccg accgtcgtca atgccgcgca atgcgccgtt gacggtctct
     8821 accagggtgt tgtcgcgtgc catcctcatc gagattaccg gtcggtagcg gttgacaccg
     8881 tgaactgcga cacgaaatgg tagagatgag atggtatatg taacacccat accagaggaa
     8941 tgagaaggat gagcgcgttg cgccgctgga ttgtcgacga cttcgagaag ggcgccggcc
     9001 gccacgacga tccgaagatc tacggcgggc caccgggcga tcccgggttg atcggcccgg
     9061 gcagcatgtc gtgggagatc aacgccgacc tcgcctcggt ctcgcaggcg gggctgccgg
     9121 caatcgtgct ggaggtcctg cacccgtcgg tgatcgccgg tgtccagaac ctgtcggact
     9181 accgcgcgga tccgttccag cgcgcccgtg ccaccctcgg atacgtcctg gccaccacgt
     9241 tcggcaacac cgaggccgcc atcgcgatca tcgaacgggt gaagcacatc cattccttcg
     9301 tcaatggcac gcgtccggac ggcgtgcctt accgtgcgtt ggatcccgag ctgatcgtgt
     9361 gggtgcacac gtgcatcccg tggatgatcc tgcgggtgtt cgaggaaacg aaaaggccgc
     9421 tgtcgcaaca tgagaaggac cgctacctat cggagcaggc ggtgatcggc cggatgagcg
     9481 gcgccgactg ggtgccggag acggcggcac agctcgacga ctatgtcgat tcaatgcggc
     9541 cgcggctgtc ggtcaacgcg cagacacggg agtttgtcga cttcctcatg acatccccct
     9601 tcttccccga cctgcccgag cggctggacc ggcagctgca ccggttcatc ttgtatgcgg
     9661 gcatgagcag ggcgcctagg tgggcgcagg agctcatcgg ttacgaccgg ccgcccatcg
     9721 cgacgcgcgc gttgacgggt ccgatgctcc gtctggacgc ccaacggctg cgatgggcgt
     9781 tcggtgcgcc gcgctatctg caactggctc agcaacgggc ggcgggcgcg aggcagggaa
     9841 aggtcgccgc ctcgtgatgg atgcccggct catcgaggtc gtcggcggcg cgagcgtcgc
     9901 cggccggatc gacgaggcga tggaccgcac cagtcaacgc attctggatg ccgcgctgtc
     9961 cgaggccgca gccgttgggc tgcaacgcat cacggtagaa gacgtggtac gccgggccga
    10021 tgtctcccgg atgacggcgt accgccgcta cccgcggcgg gacgatctcg tcgcggccct
    10081 ggtgcgccgg gagacgcagc gcttcctcgg cgcggtcgcc gacgccatcg aaggcgtcga
    10141 ggactccgac gacggcgtgg cggaggcctt catcgccgcg gtgtcgttcg ccagggagca
    10201 tccgatcctg cgccgggctg cccagaacga gcccgcgccg atcgccgatt ccgtcgagct
    10261 gttgacgatg ggctcggcgt tcatcgccga ctacatccac ggcgacgcaa ccggtgcccc
    10321 gtcccggcag gtgcgctggg tcgccgatgt gttcgcacgg ctgttcgtca cctatatctc
    10381 gatgcccccg agcgaccccg acttcaccga cgacgccgaa ctgcgacgct tcgcccacga
    10441 ggtcctgact cccatggtgc agcaagccgt ccgttagctt gtagccgtgc ggaaatgggt
    10501 gctgctcggc gcggcgatcg ccgtcgaggt ggcggcgacg ttgtctttgc gtgcctcgca
    10561 ggatcattcg gcgtggctga tcgtggtcgt ggctggctac accggcgctt tcgtgttgct
    10621 cacgttggtc cttcgcgcgg gcctgtccat cggggtggcg tatggcatct ggggcgcgct
    10681 ggggaccgtg ggcaccgcgg tgcttgcggc agcgatcttc ggcgacccgt tgacgtggcc
    10741 catcgcggtc ggcatcggcc tgatcatcgc gggcgtcctg ctcgtcgagt tgggggcgcg
    10801 gcacaccgaa gaggccgccg cgtgatgtgg gcgacgctga ccggcgccat ccttttcgag
    10861 gtcgtcgcca cggtcgcgct gcgggcgtcg gagggcttcc gcaggcggat ctggatcgtc
    10921 cccgtgacca tcggctacct ggtgtcgttc tacctgctgt ggctgacact gcggttgggc
    10981 atgcacgtcg gcatcgccta cggcgtctgg accgcgtgcg gcgtggcggt ggttgccgtc
    11041 gccgccaagg tcctcttcga cgagccgctg acctggttga tggggctggg tatcgcgttg
    11101 atcgtggga
//
LOCUS       DASYLX010000812         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3884360,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000812 DASYLX010000000
VERSION     DASYLX010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3884360"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(714..848)
                     /locus_tag="VGP27_18980"
     CDS             complement(714..848)
                     /locus_tag="VGP27_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853418.1"
                     /translation="MVTASLEEYIASPLSPISWCDNTIDCESIVKFVLLAYAIVGGDV
                     "
     gene            1029..1640
                     /locus_tag="VGP27_18985"
     CDS             1029..1640
                     /locus_tag="VGP27_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853419.1"
                     /translation="MSPNRGRGRPRSEQHRDAVLKAAMELMQEDDLRRASIDRIAQRS
                     GVSKATIYKWWPNRTAVAIDAFLDRMMTDAPVPDTGSAAEDFRLTLRGMMGFYSSRSG
                     AMYAQLVGVSQFYPAERERIRTHQVNLRRAAVTKIWHRGVARSELDPGVDPEVVLDLI
                     FGAAMYRMATGHGGLTPSDADAIVATAMRALASRTVGAGGSSL"
BASE COUNT          361 a          561 c          565 g          332 t
ORIGIN      
        1 ctcggagcta cgacctcgac gaaagccaag acgcttaccg cgcggtggcc agcgggaccc
       61 cgggccggat cgtgatcaac ccatgaccgc aatcagccct agccgcacgc gggtcgattt
      121 ccaccatcac cttctgcccg ctggtcagct ggctgaatca gtgcgggggc gcttggcgcc
      181 gattccggct ggcagggcaa cgcgcagaac ctgctgctcc gcttgaatgc gaacaccgcg
      241 gccggagccg acccagccgc gacgaaagac gtctcatgcc gctcatcgct ttcgaagaac
      301 acgtgatccg ccggtgactc atcaactatg tatggtccac caccagctac cggaatgagg
      361 ccgaggttct atggctaact atgacggttc tggtccggaa ctgaccagcg gcgaccgtgt
      421 ccacaacgcg ttacgttcgc gcatgcactc agatgtcgtc ggccatctga cagcggctcc
      481 gccacaacta gccaacgctc tgggtgtggt tctggctgtg tctgcgtgag cgggcgtggt
      541 gcagaagaaa gcaaacccaa cggcgattgc ccgaattgac cgtggcggcc ttgtcccgcg
      601 ttcatgcgcg cacctcccgc ctgcctcggc ggcggtcaag gcgcagtcga gatgtgagcg
      661 cagcgaggtg gccctcgata gcggcggcca gagctgggag ttgatgcatg ccatcagacg
      721 tcaccaccca cgattgcgta ggccagcagg acgaacttca cgatggattc gcagtcgatc
      781 gtgttgtcgc accacgagat cggactaagt ggggaggcga tatactcctc gagactggcg
      841 gtcaccacgc cgatgagttc ggcgcgtgcc tcactctcct ttgatattga gccgctgtct
      901 gagcgcgaat ttgcttgcac agcagcatgt tgcgcgtccg cttagctggt ggcaactcac
      961 ggaggcatac tcacagcgag acgtctagta attacctttg taccaacgac gggtaggaca
     1021 ctggaggaat gagcccgaat cgagggcgcg gtcgaccgcg tagcgagcaa caccgcgacg
     1081 ccgtcctcaa agccgccatg gaattaatgc aggaggacga cctgcgcagg gcgtcgatcg
     1141 accggatcgc ccaacgcagc ggggtcagca aggcaaccat ctacaaatgg tggccgaaca
     1201 gaaccgcggt agccatcgac gccttcctgg atcggatgat gaccgacgcc cccgtgccgg
     1261 acaccggatc ggcggccgaa gacttccgcc tgacattgcg cggcatgatg gggttctaca
     1321 gcagccgatc tggcgcgatg tacgcccaac tcgtcggcgt gtcgcagttc tacccggctg
     1381 aacgggagcg aatacgaaca caccaggtga accttcgccg agcagccgtg acgaagatct
     1441 ggcaccgcgg tgtcgctcgc agtgaactcg atccgggcgt cgatcccgag gtggtgctgg
     1501 atctcatttt cggggcagcg atgtaccgga tggcgactgg acacggggga ttgacgccgt
     1561 ccgacgccga cgccatcgtg gcgacagcga tgcgggcgct ggcaagccgc acagttggag
     1621 cgggcggatc ctcactgtaa atctgttggg cggcccggcg ggatcgtctt cgagctcgat
     1681 ccgtatctag ttttgttcgg tcttcacgag aattgatgca ccgctcattg tttcggattc
     1741 tggcacgtgg gcgctaccgg ctagcctccc tatgaatttc ctggatggca gcaatgacac
     1801 agccagccct ccagtccaa
//
LOCUS       DASYLX010000813         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2568131,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000813 DASYLX010000000
VERSION     DASYLX010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568131"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VGP27_18990"
     CDS             <1..159
                     /locus_tag="VGP27_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7853420.1"
                     /translation="PGDLTFSWYAVDRAGQPYVVSFQLNWPKFRSQTAAAWLLSIVHQ
                     TFDLVHAA"
     gene            complement(160..711)
                     /locus_tag="VGP27_18995"
     CDS             complement(160..711)
                     /locus_tag="VGP27_18995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7853421.1"
                     /translation="MSETVRLTLVSHAMTDAMSAGRFPTDEPLNSVGHRQVDASIELG
                     ITDSAFCGPEKRTRQTAELLGLQAIIDQRLADLDCGRWRGDVLGGVLPAELAIWLTDP
                     GRAPHGGESVVDLVNRVRDWMDTLTTRRSRLVAVTHPALIRAAILVALDAPAKSFWRI
                     DIAPVSRTVMHFRGHAWNLRSTL"
     gene            complement(728..1459)
                     /locus_tag="VGP27_19000"
     CDS             complement(728..1459)
                     /locus_tag="VGP27_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEV7853422.1"
                     /translation="MEKRIIARGLLAGAAASVAAFVFARTFVEPVIARAIAFEEAQEA
                     GHEHGVELFTRGVQANIGMGFGVLAFGVAMGALFAVAYCVSYGRLGTSSPRLLSVLLA
                     AGMFLSLYVVPFLKYPANPPAVGLDETIRARTLLYLMMVVLSAVLLAAAVWLGRRLVE
                     RFDAWSATLIAAAAYVVAVAIVMLALPTIDETPDEFPADVLYDFRLYSLGTQFVLWAI
                     IAVVFGSMAHRLLGQPERQETFSPV"
     gene            complement(1482..1697)
                     /locus_tag="VGP27_19005"
     CDS             complement(1482..1697)
                     /locus_tag="VGP27_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEV7853423.1"
                     /translation="MTSSEARKSAAPALDISAAKAAVWLTATAFLALLVLYFVGMDQG
                     ATSVFGSNTYVHEFTHDARHLLGFPCH"
     gene            complement(1918..>3601)
                     /locus_tag="VGP27_19010"
     CDS             complement(1918..>3601)
                     /locus_tag="VGP27_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV7853424.1"
                     /translation="TLVGAGAAEGAIDAASILKPKLARGELQTIGATTLDEYRKYIEK
                     DAALERRFQPVQVGEPTVEHTIEILKGLRDRYEAHHRVSITDGAMVAAATLADRYIND
                     RFLPDKAIDLIDEAGARMRIRRMTAPPDLREFDEKIADARREKESAIDAQDFEKAASL
                     RDREKQLVAQRTEREKQWRSGDLDVVAEVDDNEIAEVLGNWTGIPVFKLTEEETTRLL
                     RMEDELHKRIIGQEDAVKAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKAL
                     ANFLFGDDDALIQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLF
                     DEIEKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTSDISKAVGLGFT
                     QGGGDNNYERMKQKVNDELKKHFRPEFLNRIDDIIVFHQLTREEIIKMVDLMIGRVEK
                     QLKGKDMAMDLTDKAKALLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVGPGQ
                     LVTVDVDNWDGEGTGEDARFTFVGTKKPAKPVEEADLANAGAAGTATTTATE"
BASE COUNT          617 a         1189 c         1144 g          651 t
ORIGIN      
        1 cccggcgacc tgacattcag ctggtacgcc gtcgaccgcg ccgggcaacc gtacgtggtc
       61 agcttccaat tgaactggcc gaagttccgc agtcagaccg cagccgcgtg gctgctctcg
      121 atcgtgcacc agacgttcga cctggtgcac gccgcgtagt cacaacgtgg aacgcaggtt
      181 ccatgcatga ccgcggaaat gcatgaccgt cctgctgacg ggtgcgatgt cgatgcgcca
      241 gaacgacttc gcgggtgcat cgagcgccac cagaatcgcg gcgcgaatga gcgcgggatg
      301 tgtgaccgcg accaaccggc tgcgacgggt ggtcagcgta tccatccagt cgcgcacccg
      361 gttgacaaga tcgacgacgg attcgccgcc gtgcggcgcc ctgcccggat cggtcagcca
      421 gatggcgagt tccgccggca gtaccccgcc gagcacatcg ccgcgccatc ggccgcagtc
      481 cagatcggcc agccgctggt cgatgatcgc ctgcaagcca aggagttccg cggtttgcct
      541 ggttcgcttc tcaggcccgc agaaagcgct gtcggtgatg ccgagttcga tcgatgcgtc
      601 gacctggcgg tggccgacag agttcagcgg ttcgtcggtg gggaatcgtc cggccgacat
      661 cgcgtcggtc atggcgtgcg acacaagtgt cagccggacg gtctcactca cgcctcgcct
      721 ccgggactca taccgggctg aacgtctcct gacgctcagg ctgcccgagc agtcggtgcg
      781 ccatcgagcc gaacaccacc gcgatgatcg cccacagcac gaactgggtg cccagcgaat
      841 acagccggaa gtcgtagagc acgtcagcgg gaaattcgtc cggtgtctcg tcgatggtcg
      901 gcagtgccag catgacgatc gcgacggcga cgacataggc cgcagccgcg atcagcgttg
      961 cgctccaggc gtcgaaccgc tccaccaggc gcctgcccag ccacaccgcg gcggcgagca
     1021 acaccgccga cagcaccacc atcatcagat agagcagagt gcgcgctcgg atggtctcgt
     1081 cgagaccgac cgcgggcgga ttcgccggat acttcaggaa cggaaccacg tacagcgaca
     1141 ggaacattcc ggccgccaac agaacggaca gcagtcgcgg tgagctggta ccgaggcggc
     1201 cgtaggagac gcagtacgcc accgcgaaca aggcccccat ggcgacgccg aatgccagca
     1261 cgccgaatcc catgccgatg ttggcctgca cgccgcgagt gaacagctcg acaccgtgct
     1321 cgtggccggc ctcttgtgcc tcctcgaagg cgatcgcccg ggcgataacg ggttcgacga
     1381 aggtgcgggc gaagacgaat gccgccacgc tcgcagcggc gccggccaac aggccgcgcg
     1441 caattattcg tttttccatt tgggtgtctg cttcgagcga atcagtggca ggggaagccc
     1501 agcagatggc gagcgtcgtg ggtgaactcg tgcacatagg tattgctgcc gaacaccgac
     1561 gtcgcaccct ggtccatgcc gacgaagtac agcaccaaca aggccaggaa tgcggtcgcg
     1621 gtcagccata cagccgcctt ggcggccgag atgtccagcg cgggcgctgc gctcttgcga
     1681 gcctcagaag acgtcatgaa ggattccttt cccgggatct cgcgtcccag tcttcaaggt
     1741 cggtggcggc cgtcgggtct gactgtcaca gtggcgcgac cgttctggat tcacaccaga
     1801 ttccttcgcc cgcctgatta catcgcacat cctaaacaac cgccccgcaa tcaggcccag
     1861 gcatagcaaa agcccccgaa tctttttgat tcgggggctt ttgcgcctac gcgttaatta
     1921 ctcggtggcc gtggtggttg ccgtaccggc cgcaccggcg ttggccagat cggcctcttc
     1981 gaccggtttg gcgggcttct tcgtcccgac gaaggtgaac cgcgcatcct cgccggtgcc
     2041 ctcgccatcc cagttgtcga cgtcgaccgt caccagctgg ccgggaccga cctcctcgaa
     2101 gaggatcttc tcggagagct ggtcctcgat ctcgcgctgg atggtgcgac gcagcggccg
     2161 cgcgccgagc acggggtcga acccgcgctt ggccagcagg gccttggcct tgtcggtcag
     2221 gtccatggcc atgtccttgc ccttgagctg cttttccacc cggccgatca tcaggtcgac
     2281 catcttgatg atctcttcgc gagtcaactg atggaagacg atgatgtcgt cgatgcggtt
     2341 gaggaactcg ggccggaagt gcttcttcag ctcgtcgttg accttctgct tcatccgctc
     2401 gtagttgttg tcgccaccgc cctgggtgaa gccgagcccg acggccttgg agatatccga
     2461 cgtgcccagg ttggacgtga aaatcagcac ggtgttcttg aagtcgaccg tgcggccctg
     2521 gccatcggtg agcctgccgt cctcgaggac ctgcaacagg ctgttgtaga tctcctggtg
     2581 agccttctcg atctcatcaa agagaacaac agagaacggc ttccttctga ccttctcggt
     2641 gagctgaccg ccctcttcgt agccgacata gccaggaggc gcgccgaaca gacgcgacgc
     2701 ggtgaagcgg tcgtggaact cgcccatgtc gatctggatg agcgcgtcgt cgtcaccgaa
     2761 caggaagttg gccagcgcct tggacagctc ggtcttaccc acaccggacg ggccggcgaa
     2821 gatgaacgag ccggacgggc gcttggggtc cttgaggccc gcacgggtgc ggcggatcgc
     2881 cttggacacc gccttgacgg cgtcctcctg gccgatgatc cgcttgtgca gctcatcctc
     2941 catgcgcagc agccgagtgg tctcctcctc ggtcagcttg aacaccggga tgccggtcca
     3001 gttgcccagc acctcggcga tctcgttgtc gtcgacctcg gcgaccacat ccaagtcacc
     3061 tgagcgccac tgcttctcac gctcggtgcg ctgtgcgacg agctgcttct cgcgatcccg
     3121 caggctggcg gccttctcga agtcctgcgc gtcgatcgcg gactccttct ccctgcgcgc
     3181 gtcggcgatc ttctcgtcga actcacgcag gtctggcggc gcggtcatcc ggcggatacg
     3241 catgcgggcg cccgcctcgt cgatcaggtc gatcgccttg tcgggcagga accggtcgtt
     3301 gatgtaccgg tcggccagcg tcgccgcggc caccatggcg ccgtcggtga tcgagacgcg
     3361 gtggtgcgcc tcgtagcggt cgcgcagacc cttcaggatc tcgatggtgt gctcgacggt
     3421 cggctcaccc acctgcacgg gctggaagcg gcgctcaagg gcggcgtcct tctcgatgta
     3481 cttgcggtac tcgtcgagtg tggtggcgcc gatggtctgc agctcgccgc gggccagctt
     3541 cggcttcagg atcgaagcgg cgtcgatggc gccctcggcg gcacccgcac cgaccagcgt
     3601 g
//
LOCUS       DASYLX010000814         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1361448,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000814 DASYLX010000000
VERSION     DASYLX010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361448"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VGP27_19015"
     CDS             complement(<1..1064)
                     /locus_tag="VGP27_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244827.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HEV7853425.1"
                     /translation="MLTVPHLDRDFDYLVAAEQSDDAQPGVRVRVRFHGRLVDAFILE
                     RRSETDHVGKLGWLDRVVSAERVLTPDVRRLVDAVAARYAGTRADVLRLAIPPRHANV
                     EKEVPGEPTPATVASVDTAGWAEYGRGEQFVEALRDGRAARAVWQALPGERWTERLAE
                     IAAVSVQAGRGVLAIVPDQRDVDALYDACVRLVDESRVVALSAGLGPAQRYRRWLSVL
                     RGSARLVIGTRSAVFAPVADLGLVLVWDDGDDSLSEPRAPYPHAREVGMLRAHQLRCA
                     AMIGGFSRTAEAQAMVRSRWAHDLVAPRPVVRSRAPRVIALEDSGYAHERDPAAHSAR
                     LPSMALQATRTALDAGHPVLV"
     gene            complement(1115..1843)
                     /locus_tag="VGP27_19020"
     CDS             complement(1115..1843)
                     /locus_tag="VGP27_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEV7853426.1"
                     /translation="MAGFSSVTNPHRRTRALWLSAVLVAAFYGIYLGATALRLPSGAE
                     LTGQFWLQPAVKAAPAVLLAAAAVTHPVIRERRWLVVALAGSAAGDFLLAMPWWQPSF
                     VLGLAAFLVAHLCFLAALLPLCSASAPRSTAAAVVVAACVALLVWFWPKLIAEGMAIP
                     VTAYIAVLGAMVCAALLARLPTPWTALGAVCFAVSDAMIGINKFVLGSEALAMPIWWA
                     YATSLTLITAGFFFGRTSDLSAKR"
     gene            1923..2345
                     /locus_tag="VGP27_19025"
     CDS             1923..2345
                     /locus_tag="VGP27_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853427.1"
                     /translation="MSTQSSVDIVHAFWDEVWNAHDPAAADRFVTEDFVIVTGGETIS
                     GRENFTKWIERFLAQVTDLHLEVTESFQNADGSRVMSRWLLTGKNNGLFGTEPDQQDI
                     AMTGTAVWAVRGDGKLLTNWVERAAWEQFQRLKLEASA"
     gene            2380..>3169
                     /locus_tag="VGP27_19030"
     CDS             2380..>3169
                     /locus_tag="VGP27_19030"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853428.1"
                     /translation="MKTAPDHDILIVGAGFSGIGTAILLDKAGMSDFSVIEAADGSGG
                     TWHWNTYPGVAVDIPSFSYQFSFEQSPDWTRTYAPGRELKDYAEHCVDKYGLRSRIRF
                     NTKVAGAGFDHDTDLWRIETDSGQCVTARYLINAGGVLTTPKPPDIDGVDSFAGETMH
                     TARWDHGQDLAGKRVGIIGTGASAVQVIPEIAPIVRHLTVFQRTPIWCFPKADVPLSP
                     TARRAMRLPLGRAVQRLLSQAYVELTFPLAAQYFTVNPMAKRAPE"
BASE COUNT          567 a         1112 c         1040 g          450 t
ORIGIN      
        1 acgagaacgg gatggccggc gtcgagcgcc gtgcgtgtcg cctgcagcgc catcgagggc
       61 agccgcgcgc tgtgtgcggc ggggtcacgc tcatgggcgt atccgctgtc ctcgagggcg
      121 atcacccgcg gtgcccgcga cctcaccacg ggccttggcg ccaccaggtc gtgcgcccat
      181 cggctgcgca ccatggcctg ggcctcggcg gtgcgcgaga atccgccgat cattgcggca
      241 caacgcaatt gatgggcgcg aagcatgccc acctcgcggg cgtgcggata gggcgcgcgc
      301 ggctcggaca aactgtcgtc gccgtcgtcc cacaccagca ccagcccgag atcggcgacc
      361 ggggcgaata cggcgctgcg ggttccgatg accagccgcg ccgaaccgcg cagcaccgac
      421 agccagcgcc ggtaccgctg tgcggggccg agccccgccg aaagcgccac cacccggctt
      481 tcgtcgacga gccggacaca ggcgtcgtac agtgcgtcga catcccgttg gtcgggaacg
      541 atcgccagca ctccccggcc ggcttgcacc gagaccgctg ctatctcggc caaccgctcg
      601 gtccagcgtt cgcccggcag agcctgccag accgctctgg cggcacggcc gtcgcgaagt
      661 gcctcgacaa actgctcgcc ccggccgtat tcggcccatc ccgcggtgtc gacggatgcg
      721 acggtggccg gtgtgggttc gccgggcact tccttttcga cattggcgtg ccgcggcgga
      781 atcgccagcc gtaacacgtc ggcgcgggtg ccggcgtagc gcgcggcgac ggcatcgacc
      841 agcctgcgca catcgggggt cagcacccgc tctgcggaga ccacccggtc cagccagccc
      901 agcttaccga catgatctgt ctccgaacgc ctttcgagga tgaacgcgtc gaccagccgg
      961 ccgtggaagc gcacccgaac gcggacaccg ggctgcgcgt cgtcggattg ttcggcggcg
     1021 acgaggtagt cgaagtcgcg atccaggtgc ggcaccgtca gcatcggcag cacccgggcg
     1081 atcggctcgt gctccgccgc ggtcttgctc acacctaccg tttagcagac agatcggacg
     1141 tccggccgaa aaagaagccc gcggtgatca gcgtcagcga cgtggcgtac gcccaccaga
     1201 tcggcatcgc gagcgcctcg gaccccagca cgaacttgtt gatgccgatc atggcgtccg
     1261 acaccgcgaa acacaccgcc cccagcgccg tccacggcgt cggcagccgc gccagcaggg
     1321 ccgcgcacac catcgcgccg agtaccgcga tgtaggccgt caccggaatc gccatgcctt
     1381 cggcgatcag cttgggccag aaccagacga gcagggcgac gcaggccgcc accaccacag
     1441 ccgccgccgt cgatctcggc gccgaagcgg agcacagcgg gagcagcgcg gccaggaagc
     1501 acagatgggc gacgaggaac gcggcgagcc ccaacacgaa cgacggctgc caccacggca
     1561 tcgccagcag gaagtcaccc gcggccgacc ccgccagtgc gacgacgagc cagcgtcgct
     1621 ccctgattac cgggtgagtg accgcagccg cagccagcag gaccgccggg gccgccttga
     1681 ccgccggctg caaccagaac tggccggtga gctcggcgcc cgacggcagt ctcagcgcgg
     1741 tggcgccaag gtatatgccg tagaaggcag cgaccagcac ggccgacagc cacagtgccc
     1801 gggtgcgccg gtgcgggttg gttaccgagg agaagccggc cattccggca tcggtggacg
     1861 ccattctgct gaaggtacac agacctcgga ctattcacat cggccagggc gttcgactgt
     1921 caatgagtac gcagagttcc gtcgacatcg tgcacgcctt ctgggacgag gtctggaatg
     1981 ctcacgaccc cgccgccgcg gaccggttcg tcaccgagga cttcgtcatc gtcaccggcg
     2041 gcgagaccat ctcgggacga gagaatttca cgaagtggat cgaacgattc ctggcacagg
     2101 tgaccgacct gcatctcgaa gtcaccgagt cgttccagaa cgccgacggt agccgtgtga
     2161 tgtcacgctg gctgctgacc gggaagaaca acgggctgtt cggcaccgaa cccgaccaac
     2221 aggacatcgc catgaccggc accgccgtgt gggcggtgcg tggggacggc aagctgctga
     2281 cgaactgggt cgagcgggcc gcgtgggagc agttccagcg gcttaagctc gaggcatccg
     2341 cgtaacaaag gggccgcgaa cgagaggagc tcgccgggga tgaagacagc ccccgatcac
     2401 gacatcctca tcgtcggtgc cggattctcc ggaatcggta ccgcgatcct gctcgacaag
     2461 gcgggaatga gtgacttttc ggtgatcgag gcagccgacg gctccggtgg gacatggcac
     2521 tggaacacct atcccggtgt cgcggttgac attccgtcgt tttcctacca gttctccttc
     2581 gagcagagcc ctgactggac acgcacctat gcacccggcc gcgagctgaa agactatgcg
     2641 gagcactgtg tcgacaagta cggcctgcga tcgcggatcc ggttcaacac caaggtggca
     2701 ggcgccgggt tcgaccacga caccgacttg tggcgcatcg agaccgactc cggtcagtgc
     2761 gtcaccgcgc ggtacctgat caacgcgggc ggcgtgctga ccactccgaa gccgcccgac
     2821 atcgacggcg tcgactcgtt cgcgggcgag accatgcaca ccgcccgctg ggaccacggt
     2881 caggacctcg ccggcaagcg cgtggggatc atcggcaccg gcgcctccgc cgttcaggtg
     2941 atccccgaga tcgcgcctat cgtcagacac ctcaccgtct ttcagcgcac gccgatctgg
     3001 tgcttcccga aagccgacgt cccactgtcg cccaccgccc ggcgcgcgat gcgcctgccc
     3061 ctcggcaggg ccgtgcagcg gctgctcagc caggcctacg tcgagctgac gtttcctctt
     3121 gccgcgcagt acttcacggt caacccgatg gccaaacggg cgccggagt
//
LOCUS       DASYLX010000815         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_814290,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000815 DASYLX010000000
VERSION     DASYLX010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814290"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1979
                     /gene="lepA"
                     /locus_tag="VGP27_19035"
     CDS             132..1979
                     /gene="lepA"
                     /locus_tag="VGP27_19035"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEV7853429.1"
                     /translation="MPITSFADQTFTAPAQIRNFCIIAHIDHGKSTLADRMLQLTGVV
                     AERDMRAQYLDRMDIERERGITIKAQNVRLPWNVDGTDYVLHLIDTPGHVDFTYEVSR
                     ALEACEGAVLLVDAAQGIEAQTLANLYLALDRDLHIIPVLNKIDLPAADPDRYAAEIA
                     HIIGCEPGDVLRVSGKTGEGVADLLDHVVREVPAPQGDADAPARAMIFDSVYDIYRGV
                     VTYVRVVDGKILPRERIKMMSTGTTHELLEVGIVSPDPKAAAGLGVGEVGYLITGVKD
                     VRQSKVGDTVTSARHGATDALTGYREPKPMVYSGLYPVDGSDYPDLRDALDRLQLNDA
                     ALVWEPETSVALGFGFRCGFLGLLHMEITRERLEREFDLDLISTSPNVVYRVEKDDGT
                     ELVVTNPSDWPEGKVRRVFEPVVKTTIIAPSEFIGTIMELCQSRRGELGGMDYLSPER
                     VELRYTMPMGEIIFDFFDSLKSRTRGYASLDYEEAGEQEADLVKVDILLQGEAVDAFS
                     AIVHRDSAAAYGNKMTTKLKELIPRQQFEVPVQAAIGSKIIARENIRAIRKDVLSKCY
                     GGDITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSSDAAGDKPKK"
     gene            1976..2671
                     /locus_tag="VGP27_19040"
     CDS             1976..2671
                     /locus_tag="VGP27_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7853430.1"
                     /translation="MTARVLLVGIVVAAATALSGCVSTVSGIAVRNANTVPSDVPRLD
                     KSALDKAVLGIDEINDIMGTTDLEVTSDIDNMTDSSDKVSDPDCLGAMFGAEEPVYKG
                     SGWTAVRDIVAREPDQDNDHWVEQTVVLYPQAANANRFLEKSRSIWQKCAGSSLAVNT
                     EDTSSLWQFGDVSGDDLVTQMATQEDADGWGCQHALVAVSNLTAETWACGYSVNDEAS
                     TMAVDIVNNAAEK"
     gene            2763..3833
                     /locus_tag="VGP27_19045"
     CDS             2763..3833
                     /locus_tag="VGP27_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEV7853431.1"
                     /translation="MIVHSSAPFNSEPSRSALAEADITALDTFYTRNHGQIPGVVERD
                     WRLSIAGLIDRAQTVTFDELTTRFEAHTLVATLQCAGNRRAGFNEIRNIEGEDPWGAG
                     ATSTAEWRGARLADVLESAGVRNEDGLHVAFEAPDIAEEATPPQPYGSSIPLSKALSS
                     EVLLAWEMNGGPLPSLHGGPVRVVVPGYIGARSVKWLTAMTVQDAPSANYFQAQAYHL
                     LPADADPDTAGPNTGIPLSSVAVTSDILVPDDGDSIPAGPFTIRGYAFAGDDRGIARV
                     DVSLDGGASWRQADLEPQRSPWSWRFWTLDVEIAPGPLHVTARAWDNTGALQPESVEA
                     LWNPKGYANNSWARMHATGISG"
     gene            3846..>4756
                     /locus_tag="VGP27_19050"
     CDS             3846..>4756
                     /locus_tag="VGP27_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7853432.1"
                     /translation="MKVAIIETSGLGDRSYIVTDGNTAVVIDPQRDVDRVLTAVDGAR
                     ITHVLETHIHNDYVTGGLELSRIVAADYVVPEGEDLGYSAHRVADGDTIESGPIVLRV
                     IHTPGHTQHHVSYELRDGTGETVGVFTGGSLLFGSTGRTDLVDEAETEALTRAQYHSV
                     QRLAEELPDGVEVYPTHGFGSFCSASSVDVDSSTIGRQRQSNPALTKDEQDFVDGLLA
                     ELSAYPSYYEHMGVINSEGPAAADLSLPKPIDPDDLRRRLESGEWVVDLRRRVAFAAG
                     HLKGSLGFELSGSFVTYLGWLHPWGTPL"
BASE COUNT          894 a         1525 c         1575 g          762 t
ORIGIN      
        1 gaaacgtcct ccattgcgac gccatattcg cagcataggt gaaggagaaa tcgcgcgatt
       61 gtgtcgccgt gccgccggct cgataccctg gaagggcata gcgcgcccgc tatacaaagc
      121 ctgaccagga gattcccata accagtttcg ccgaccaaac cttcaccgcg ccggcgcaga
      181 ttcggaactt ctgcatcatc gcccacatcg accacggcaa gtccacgctg gccgaccgga
      241 tgctgcagct caccggcgtc gtcgccgagc gtgacatgcg tgcgcagtac ctggaccgga
      301 tggacatcga gcgggaacgc ggcatcacca tcaaggccca gaacgtgcgg ctgccgtgga
      361 acgtcgatgg caccgattac gtgctgcatc tgatcgacac cccgggccat gtcgacttca
      421 cctacgaggt gtcgcgggcg ctcgaggcgt gcgaaggcgc agtgctgcta gtcgacgccg
      481 ctcagggcat cgaggcgcag acgctggcga acctgtacct ggcgctcgac cgcgacctgc
      541 acatcattcc ggtgctcaac aagatcgacc tgcccgccgc cgaccccgac cgctatgccg
      601 ctgaaatcgc ccacatcatc ggctgcgagc ccggcgacgt gcttcgggta tccggcaaga
      661 ccggcgaggg cgtcgccgac ctgctcgacc acgtcgtgcg tgaggtgccc gccccgcagg
      721 gcgacgccga cgcaccggcc cgggctatga tcttcgactc cgtctacgac atctaccgcg
      781 gcgtcgtcac ctacgtccgt gtcgtcgacg gcaagatcct gccgcgcgaa cgaatcaaga
      841 tgatgtctac cggcaccacc cacgaactac tggaggtcgg catcgtgtcg cccgacccca
      901 aggcagccgc cgggctcggt gtcggcgagg tggggtatct catcaccggc gtgaaggatg
      961 tccgccagtc gaaggtcggc gacaccgtga cttccgctcg ccatggcgcc acggacgcgc
     1021 tcaccggata ccgcgagccg aagccgatgg tctattccgg gctgtatccc gtcgatggct
     1081 ccgactatcc ggacctgcgg gacgcgttgg acaggctgca gctcaacgac gcggcactgg
     1141 tctgggagcc ggagacgtcg gtggcactgg ggttcggctt ccggtgtggc ttcctcgggc
     1201 tgctgcatat ggaaatcacc agggaacggc tggagcgaga gttcgacctc gacctgatct
     1261 cgacatcgcc gaacgtcgtg taccgcgtgg agaaggacga cggcaccgag ctcgtcgtca
     1321 ccaacccgtc ggactggccg gaaggcaagg tgcgcagggt gttcgagccg gtggtcaaga
     1381 cgacgatcat cgcgccgagc gagttcatcg gcaccatcat ggagctgtgc cagtcgcgcc
     1441 gcggcgagct cgggggcatg gactacctgt cgccggagcg ggtcgagctg cgctacacga
     1501 tgccgatggg tgagatcatc ttcgacttct tcgactcgct gaaatcgcgc acccgcggct
     1561 acgccagcct cgactacgag gaggcaggtg agcaggaggc cgacctggtc aaggtggaca
     1621 tcctgctgca gggtgaggcc gtcgacgcgt tcagcgcgat cgtgcacagg gactccgctg
     1681 ctgcgtacgg caacaagatg accaccaagc tcaaggaact gatcccacgc cagcagttcg
     1741 aggttccggt gcaggctgcc atcggctcga aaatcattgc ccgcgagaac attcgcgcca
     1801 tccgcaagga cgtgctctcg aagtgctacg gaggtgacat cactcgtaag cgcaagctgc
     1861 tggagaagca gaaggaaggc aagaagcgga tgaagaccat cggtcgcgtc gaggttccgc
     1921 aggaggcctt cgtcgccgcg ctgtccagcg acgccgcggg tgacaagccg aagaaatgac
     1981 agcgcgagtt ctgctcgtgg gaatcgtggt cgcggccgcg acggcgttat ccggctgcgt
     2041 gagtacggtt tccggcatcg cggtgcgcaa cgcgaatacg gtgccgtcgg atgtgccgcg
     2101 gctggataag tcggctctgg acaaggcggt cctaggcatc gacgagatca acgacatcat
     2161 gggcaccacc gacctcgagg tcaccagcga catcgacaac atgaccgatt catcggacaa
     2221 ggtgtccgac cccgactgcc tgggcgcgat gttcggcgcc gaggagcccg tctacaaggg
     2281 cagcgggtgg acggcggtgc gtgacatcgt ggcccgcgag cccgaccagg acaacgacca
     2341 ctgggtcgag cagaccgtcg tgctctaccc gcaggccgca aacgccaacc ggttcctgga
     2401 gaagtcgcgg tcgatctggc agaaatgcgc aggttcgtcg cttgcggtga acaccgagga
     2461 caccagctcg ctgtggcagt tcggcgacgt ctccggagac gacctggtca cccagatggc
     2521 gacgcaggag gatgccgacg gctggggatg ccagcacgcc ctcgtggcgg tgtccaacct
     2581 gacggcggaa acgtgggcct gcggctacag cgtcaacgac gaggcgtcga cgatggccgt
     2641 cgacatcgtc aacaacgccg cggagaagta acagagcaaa acttctgatc agccggagtt
     2701 caacgcgcgc gggctccgga cacaccgata gattgagcaa ggtgtggggc aagcgaccgg
     2761 agatgatcgt gcacagcagt gcgccgttca actccgagcc atcccggtcc gcgctggcgg
     2821 aagcggatat caccgcgctg gacacgttct atacgcgcaa tcacggtcag attccgggtg
     2881 ttgtcgaacg cgattggcgg ctgagcatcg ctggactcat agaccgggca cagaccgtca
     2941 ccttcgacga actcaccacc cgcttcgagg cccacactct ggtggcgacg ctgcagtgcg
     3001 caggcaaccg ccgcgcggga ttcaacgaga tccgcaacat cgagggcgag gatccgtggg
     3061 gggcgggggc gacttcgacc gctgaatggc gcggtgcgcg tctcgccgac gtgctggaga
     3121 gcgctggagt gcgaaacgag gacggtctgc acgtggcctt cgaagcgccc gacatcgccg
     3181 aggaagcgac gccgccgcag ccgtacggca gctccattcc gctgtcgaag gcattgtcgt
     3241 cagaagtact gctggcgtgg gagatgaacg gcggtccgct gcccagcctg cacggcggtc
     3301 cggtccgagt cgtggtcccg ggctatatcg gcgcccgcag cgtcaaatgg ctcaccgcca
     3361 tgactgtgca ggatgcgccg tccgccaact actttcaggc gcaggcgtat cacctcctgc
     3421 ctgcagatgc cgaccccgac accgccggtc cgaacacggg catcccgctg tcgtcagtcg
     3481 ctgtcacctc cgacattctt gtgccggatg acggtgactc gatacccgcg ggacctttca
     3541 cgatccgcgg gtacgccttc gccggggatg atcgcggcat cgccagggtc gacgtgtctc
     3601 tcgacggcgg agcgtcgtgg cgccaggcgg acctcgagcc gcagcgcagt ccgtggtcgt
     3661 ggcggttctg gacgctggac gtggagatcg cacctgggcc actgcacgtc acggccaggg
     3721 cctgggacaa caccggcgcg ctccaacccg agtcggtaga ggcgctgtgg aatccgaaag
     3781 gctatgcgaa caactcgtgg gcgaggatgc acgcgacggg gatttcgggg taatccggta
     3841 gcaccatgaa ggtagccatc atcgagacgt cgggcctggg cgatcgaagc tacatcgtca
     3901 ccgatggcaa caccgctgtg gtcatcgatc cgcagcgtga tgtcgaccgg gtgttgaccg
     3961 ccgttgacgg tgctcgcatc acccacgtgc tggaaaccca catccacaac gattacgtga
     4021 ccggcgggct ggagctgtcg cggatcgtgg ccgccgacta cgtggtgccc gagggggagg
     4081 atctcggcta ttcggcacac cgggtcgccg acggcgacac catcgagtcc gggccgatcg
     4141 tgctgcgggt gatccacaca ccgggccaca cgcagcacca cgtcagctac gaactgcgcg
     4201 acgggaccgg agagacagtc ggcgtgttca ccggaggatc gctgctgttc ggcagcaccg
     4261 gccgcaccga cctggtcgac gaggccgaga cggaagcact gacgcgcgcg cagtaccact
     4321 ccgtccagcg ccttgcggag gaacttcccg acggcgtgga ggtgtacccg acgcacgggt
     4381 tcggcagctt ctgctccgct tcgtcggtcg acgtggactc gtcgacgatc gggcggcagc
     4441 gccagagcaa tcccgcgttg accaaggacg agcaggactt cgtcgacgga ctgctggcag
     4501 aactttcggc gtacccctcc tactacgagc acatgggtgt gatcaacagc gaaggccctg
     4561 cggccgcgga tctttcgctg ccgaagccga tcgaccccga cgatctgcgt cgccggctgg
     4621 agtcggggga gtgggtcgtc gacctccgcc ggcgggtggc gttcgccgcc ggacacctca
     4681 agggcagtct cggattcgag ttatccgggt cattcgtcac atacttgggc tggctgcacc
     4741 cgtggggtac cccgct
//
LOCUS       DASYLX010000816         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2238615,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000816 DASYLX010000000
VERSION     DASYLX010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238615"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VGP27_19055"
     CDS             complement(<1..808)
                     /locus_tag="VGP27_19055"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV7853433.1"
                     /translation="MLQSGVGTVAACKLLGIGRKTGYRWRAENRGLPPARLAEAAHSS
                     RYLSQLERQRIATLRDRGLGVREIARRLGRSPSTISRELRRHLRPHDRGLYDGDLAHA
                     RARERLRRPGRPVLAREPELRQIVQDKLTEEWSAAQIAAHLRETYPDRPAWHLCHETI
                     YQALYHGGKGGLSTTLTKKPRTGRPLRKRRRTPMARTPRFIAPGRLIDARPGVVEERA
                     RIGDWEGDLIVGRSSRSAIGTLVDRRTGYLRLVHLPDGHSAEQLRVAMLPV"
     gene            1407..3131
                     /gene="argS"
                     /locus_tag="VGP27_19060"
     CDS             1407..3131
                     /gene="argS"
                     /locus_tag="VGP27_19060"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV7853434.1"
                     /translation="MTNLENLLIDRFAVAFEAVAGERVDPVVRRSQHADFQADGALPL
                     ARKLGRAPREIATDVLARVDLSGLVESATISGPGFINVTIEAAALAGLLTEMQSDDRL
                     GIPMAASPETVVVDYSAPNVAKEMHVGHLRSTVIGDAVARLLTWLGHDVPRANHVGDW
                     GTPFGMLIEHLLDLGETEAAHELSVGDLNGFYRAARVKFDADPGFADRSRARVVALQS
                     GDQTTLRLWKLLVAESERYFLTVYDTLDVMLTSDDFFGESFYNPMLAPVVDELDRLGL
                     LHESDGAKVVFPAGFTGRDGEPMPLIVRKRDGGYGYGATDLATIRYRTQDLKATRLLY
                     VVGSPQHQHLQMVYQTAREAGWLAPPARATHIGFGQVLGPDGKKFASRAGDTIKLADL
                     LDEAISRASAIITEKNPDLDEPTRAAVARAVGIGAIKYADLSSDWIKDYVFAWDRMLA
                     TTGDTAAYLQYTYARIQSIFRKGAVTPERSGAAIRVVEPAEHALALELLGFPAIIDEV
                     AETLQFHKLTVYLQGLAGAYTTFYDQCPVLKADDETRASRLVLCALTGRVITQGLALL
                     GITTPDRM"
     gene            complement(3141..3275)
                     /locus_tag="VGP27_19065"
     CDS             complement(3141..3275)
                     /locus_tag="VGP27_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853435.1"
                     /translation="MSFVEGWIKPMSAPTDGLAQPLPERLARLLGLDAVVETINGVRI
                     "
     gene            complement(3275..>3571)
                     /locus_tag="VGP27_19070"
     CDS             complement(3275..>3571)
                     /locus_tag="VGP27_19070"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV7853436.1"
                     /translation="LPGQPVDDWHIDYPYNEMPTLVTGSILGLQCVLALDRFDEVNGA
                     TQLLRGTHSAPRRPAEPFGMPVSFEAEPGTLLVMAAATCTARASTDPAIRARRC"
BASE COUNT          562 a         1146 c         1234 g          629 t
ORIGIN      
        1 gtaccggaag catcgcgacg cgcagctgtt cagcgctgtg cccgtcgggc aggtgcacga
       61 gccgcaggta gccggtgcgc cggtcgacca gggtgccgat cgccgagcga ctggatcgcc
      121 ccacgatcag gtcgccctcc cagtcaccga tccgggcgcg ctcctcgacc acgcccgggc
      181 gggcgtcgat cagccggccc ggcgcgatga accgtggcgt gcgcgccatc ggcgtgcgcc
      241 ggcgcttgcg cagcggtcgc ccggtgcgcg gcttcttggt caacgtcgtg ctcaggccac
      301 ctttcccgcc gtgatagagg gcctggtaga tcgtctcgtg acatagatgc caggccggcc
      361 ggtccgggta ggtctcccgc aggtgcgccg caatctgcgc cgcactccac tcctcggtga
      421 gcttgtcctg cacgatctgg cgcagctccg gctcgcgggc cagcaccggc cggcccggcc
      481 ggcgcagtcg ttcccgggcg cgagcgtgcg ccaggtcgcc gtcgtaaagg ccgcggtcgt
      541 gcggccgcag atggcggcgc agctcccggc tgatcgttga aggcgagcgg cccagtcgac
      601 gagcgatctc ccgaaccccc agcccgcggt ctcgcagggt ggcgattcgt tgtcgttcca
      661 gctgcgacag gtagcggctc gagtgggccg cctcggccag ccgtgccggt ggcagaccgc
      721 ggttctccgc tcgccaccga tagccggtct tgcgcccgat gcccaggagc ttgcacgcgg
      781 cgaccgtgcc tacgccggac tgcagcagct tccagttctc ggactcgagg tcaagttgtc
      841 atttgcggcc atgccgtccc actcgcgcac ccctcgctac caggagtgtt gcgacaagcc
      901 atggaacccg cccgccgcca aggggtcaga cttcgcccgc cgttgacatt cgccggctac
      961 cgcgtccgtg gcgagctatc caaccctgcg tccagcccca gaaactgatc atgatgaccc
     1021 gggcgggcta atgatcatgg cagcacccgg tatccagcta cggcacagag ggcttgctcc
     1081 gtcgccacga cgaatggccc gcatcgaccc aggcccccgc acggtgtggt ctggccaccg
     1141 tgcaggctga tgggtcgaca gggtggtgtg agcggcggcg acccggcgcc gttgatccgg
     1201 tatgcttgac actatgtttt cgcccgcgat gctcttcgaa cgcccgttgg gcgcttcgat
     1261 tgtgcaactc cgggctagcg ccgtcatgcg acgacggttc cggtcagcgc gctgagccag
     1321 tcgtactggt ggcgctgtta cggcccaaat tcttgaacga tcgtttgaat cctgcccgcg
     1381 cgcgtgttcc tagaaggcag tcagtcgtga cgaacctgga aaatcttctc atagatcggt
     1441 tcgcagtcgc ctttgaggcg gtcgccggtg agcgcgtgga cccggtcgta cgacgctcgc
     1501 agcatgccga cttccaggcc gacggcgcgt tgccgcttgc tcgtaagctc ggccgcgccc
     1561 ctcgggagat cgcgactgat gtgcttgccc gcgtcgacct gtccgggctg gttgagtcgg
     1621 cgacgatctc gggtccggga ttcatcaacg tcacgatcga agcggcggcg ctcgcgggac
     1681 tgctgacgga gatgcagtca gatgatcggc tcggtatccc gatggcggcg tcaccggaga
     1741 cagtggttgt ggactactcg gcgccgaacg tggcgaagga gatgcacgtt gggcatcttc
     1801 gctcgaccgt cattggagac gccgtagccc gtttgctgac ctggctgggt cacgacgtgc
     1861 cgcgggcgaa ccatgtcggg gactggggta cgccgttcgg gatgctcatc gagcacctac
     1921 tcgatcttgg cgagaccgag gccgcccacg agctgtcggt cggggatctc aacggcttct
     1981 accgggcggc gcgagtcaag ttcgacgccg atcccgggtt cgctgaccgg tcgcgggcgc
     2041 gggtcgtggc gttgcagtct ggcgaccaga cgacgctgcg gttgtggaag ctgctggtcg
     2101 ccgagagcga gaggtacttc ctgactgtct acgacaccct cgacgtcatg ttgaccagtg
     2161 atgacttctt cggggagagc ttctacaacc cgatgttggc gccggtcgtt gacgagctcg
     2221 atcggctggg actgcttcac gagagcgacg gagcgaaggt cgtcttcccc gctgggttca
     2281 ctgggcggga tggcgagccg atgccgctga tcgtgcgcaa acgtgacggc ggctacggct
     2341 atggggcgac ggaccttgcc acgatccggt accggacgca agacctcaaa gcgacccggc
     2401 tgctgtatgt ggtcggttcg ccgcagcatc aacacctgca aatggtgtac cagaccgcgc
     2461 gggaggctgg ctggctcgcg ccgccggctc gggcgacgca catcggcttc ggccaagtgc
     2521 tgggtcccga cggcaagaag ttcgcctcgc gggcaggcga cacgatcaaa ctcgcggacc
     2581 tcctcgacga ggcgatcagc cgggcatcgg cgatcatcac ggaaaagaat cccgatctgg
     2641 acgaaccgac ccgggctgcg gtggcccgcg cggtgggcat cggcgcgatc aagtacgccg
     2701 acctgtccag cgactggatc aaggactacg tgttcgcatg ggacaggatg ctggccacca
     2761 ccggggatac cgccgcctac ctccagtaca cctatgcccg tattcagtcg atcttccgta
     2821 agggcgcggt gacccccgaa cgcagcggcg cggccatacg ggttgtggag ccggccgagc
     2881 atgcgctcgc gttggagcta ctcggcttcc cggcgatcat cgatgaggtc gctgagacgc
     2941 tccaattcca caagctgacc gtctacctgc agggcctggc gggggcgtac acgacgttct
     3001 acgaccagtg cccggtgttg aaggccgacg atgaaacccg tgcgagtcgc ctcgttctgt
     3061 gcgcccttac tggccgggtg atcacgcaag gactggcgct gctgggcatc acgacaccgg
     3121 atcgcatgta ggcagctggt tcagattcgg acgccattga tggtctccac aacggcgtcg
     3181 agcccgagca ggcgcgcgag tcgttcgggg aggggttggg cgagcccgtc ggtgggagcg
     3241 gacatgggtt tgatccagcc ctcgacgaag ctcatcaaca tcgccgtgcg cggatcgctg
     3301 gatcggttga ggcccgagcg gtgcaggtgg cagcggccat gacgagcagc gtcccgggtt
     3361 cggcctcgaa gctgacgggc atgccgaagg gctcggcagg gcgccttggc gcgctgtggg
     3421 ttccgcgtag cagttgggtg gcgccgttga cttcgtcgaa gcgatcaagg gcgagcacgc
     3481 attgcaggcc caggatcgag ccggtgacga gagtgggcat ctcgttgtag gggtagtcga
     3541 tgtgccagtc gtcgacgggt tgcccgggaa g
//
LOCUS       DASYLX010000817        10026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3226842,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000817 DASYLX010000000
VERSION     DASYLX010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226842"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VGP27_19075"
     CDS             complement(<1..674)
                     /locus_tag="VGP27_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7853437.1"
                     /translation="MLSLARLSCFVAVAEELHFGRAAERLHMTQPPLSRQIQQLESEL
                     GVQLIERSTRAVTLTPAGVAFLPDARRILQLAEGAAQTVKRIPAGDLGTVVIGFTAAS
                     AHVVLPRLLAAAREKLPDVKLDLREMVTAVQLEGLMTGEVDLGMARPPIRRPGLVSRP
                     LLHEQLIAALPEAHPLVGLGRGLTLNDLDGQDVVMYSPVQARYFNELLISTFTIAGAT
                     PRYVQTV"
     gene            782..2416
                     /locus_tag="VGP27_19080"
     CDS             782..2416
                     /locus_tag="VGP27_19080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7853438.1"
                     /translation="MAKSVEKGCALVAAEHRQLSEGLTGEGYRRLVERSPDGICVHAN
                     GRVVFVNDAGVRLMLADSSDQIVSRPITDFVTRESIPPMQSGVARLRELGDCTPHYPA
                     QMVRVDGSLLAVEIVIAMTIWEDEPAHQIITRDVSARHASESALRYQAALVNHVSDAI
                     IGTTATGVVTSWNPAAEQIYGRPFAEAKGHPMSELVGAELDPTAVVASGGVVHSTHRS
                     ADGAPLDVRVSAAAMNDGYVFVCCDLTALHRAEQHFKAIVASMVEGVVLIGKDRRIKS
                     INPAAINIMGAGPEYVGSDFFAITDQFHFLDADGVNIPPARRPVRAVLRTGVPFFNQV
                     FGFDQFGGERVWLMCSCRLLNPEILGQSDMLMSFVDVTAERKTADKILFYATHDALTE
                     LPNRVSVLRRLKKALAAPEPGDQLRAVLFMDIDDLKSTNDTLGHTAGDDLLRVAAGCL
                     RRVVAADDVVGRLGGDEFVVLVARDVAEGELDDMVNRLRIELNTPALIGGTSTPIGAS
                     IGVVEVHRDEKRTPDEILRDADLAMYEAKRARRRDV"
     gene            complement(2429..3694)
                     /locus_tag="VGP27_19085"
     CDS             complement(2429..3694)
                     /locus_tag="VGP27_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase family protein"
                     /protein_id="HEV7853439.1"
                     /translation="MAPGPIRITGARITPIAFGDPPLLNTVGVHQPFALRAIIQLDTD
                     AGLVGLGETYADETHLDRLRAAADAITGLDVFGLNAIRAAIADRLTGDSRAVGTAGMI
                     TTASAVDQVLSPFEVACLDVQGHATGRPVSDLLGGKVRDAVPFSAYLFYKWAAHPGAE
                     PDAYGEALDPDGLVAQAKRIVDEYGFTAIKLKGGVFPPEEEVAAIEALHAAFPNHPLR
                     LDPNAAWTPQTSVKVASGLAGILEYLEDPTPGLDGMAEVAAQAPMPLATNMCVVAFDH
                     LAPAVARNSVHVVLSDHHYWGGLQRSRLLAGICEAFGLGLSMHSNSHMGISLAAMVHL
                     AGATPNLTYACDTHWPWKTEEIVKPGALEFLDGAVAVPSTPGLGVELDVDALGALHEQ
                     YLRCGIRDRDDTGYMQTVDPGFEASSPRW"
     gene            complement(3697..4644)
                     /locus_tag="VGP27_19090"
     CDS             complement(3697..4644)
                     /locus_tag="VGP27_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEV7853440.1"
                     /translation="MSAARPVFAGVLFFPVTPFTESGAVDLDALARHIANGVDAGPGG
                     VFIACGTGEFHALDPQEFEPVVRTAVEVVAGRVPVYAGAGGSIAQAKQFAKAAKDAGA
                     DGLLLLPPYLVEMPQAGLVAFVRDVAAATDLPVIVYNRNNARFTEASAIAVSKIPNVI
                     GFKDGTGDLDKVARIVRAVTDALEGEAKDFLFFNGMPTAETTQQAYRAIGVPLYSSAT
                     FAFAADIALAFYNALETGNEVLIDALSRAFFHPLVRLRDTVPGYAVSLVKAGVTLEGT
                     PAGPVRPPLVMPSADDLAELRSIIAAGRAVLADALAEAR"
     gene            complement(4670..6265)
                     /locus_tag="VGP27_19095"
     CDS             complement(4670..6265)
                     /locus_tag="VGP27_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEV7853441.1"
                     /translation="MTATMPETHLTGQMIIAGESVRGSGKQIRAFDPTAGHELEPAYS
                     YGGASDVDAASAAAAEAFAEYRCTTSEQRAQFLETIAANIETVKDALVERAVAESGLP
                     QARLNGEVGRTTGQLRLFAGVLREGSWNAARIDPALPDRTPLPRPDIRQRSIPLGPVA
                     VFGASNFPLAFSVAGGDTASALAAGCPVVVKAHDAHPGTSELVGRAISDAVAAAGLPA
                     GTFSLLYGFGPELGVTLVTDPRIKAVGFTGSRSAGMALVAAAAGRPEPIPVYAEMSSI
                     NPVFLLDGALAGHGADLGRAFVNSLTLGSGQFCTNPGLVIALDGKGLDTFVTAARDAL
                     AQSSATPMLTPGIADAYTNGVTELSAEADLIGRGEAGDSQTGCRAALFATDVKTFLSS
                     KTLQGEVFGSSSLIVRCSDPAEMRSVAEHIEGQLTATVHADESDYDEVRQILPLLELK
                     AGRILFDGWPTGVEVCHAMVHGGPSPATSDSRTTSVGARAIERYLRPVCYQDMPKSLL
                     PSVIADGNPDNLWRRIDGRLQQD"
     gene            6487..7719
                     /locus_tag="VGP27_19100"
     CDS             6487..7719
                     /locus_tag="VGP27_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853442.1"
                     /translation="MDLVLGLSVTSTAVRWVLVEGVTGDGDAVDRGVLDVVDASTFDA
                     EALLTVLLDANEAAVESGVHAVGVTWTPAGEAVAGAILEALQARGLSNAIAVSEVEAA
                     EALASGIADIAGYESVAVCVFEPDYTLVAMVNADGVSADPQNLPDDVELAGSVIALDL
                     NDRQLDAVFVVGSANLEPVVSAMDAVAAAPVISSAEADMAMARGAAVASAMAVNSMDG
                     GSTSLLGLRGMSRTTVLTSVVVAAAATLVISLSVVLGLQATSDSPSETKNANATGEQV
                     KEPTVPTEAKAALAAPKLQKRPAAAPAPKPAPAAVPPAPEPAAPPIVQTMVAAPPAPE
                     AVPDAGPPAANLPDTWVPPAATPPVPAYAPPVPAYVPPAQPQVPAVQPEPRLRDKIIE
                     RIPIINRFHDPQPMYPTN"
     gene            complement(7811..8509)
                     /locus_tag="VGP27_19105"
     CDS             complement(7811..8509)
                     /locus_tag="VGP27_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV7853443.1"
                     /translation="MAAGMLQCGRTPPTAPVSSAPPAATTTTPPTPSAPPPSSPTSAS
                     PAFAPAAVHPVTAAELGATWRPGCPVGPELLRRVNLDYVGFDGQTHRGELVVHQDVVA
                     DVIAIFEQLQVLRYPIATMRTVDHYPGAEDELSMEDNNTSAYNCRLMPSGRAWALHAY
                     GRAIDINPLLNPFVDREGTLEPKTAGPYLDRNRTDPGLLHAGDPVVRAFTDRGWRWGG
                     DWRTPKDYQHFEHS"
     gene            8840..9784
                     /locus_tag="VGP27_19110"
     CDS             8840..9784
                     /locus_tag="VGP27_19110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7853444.1"
                     /translation="MGERDTAHQPATRDVTALTGVKEPAFEAIVHMLDDGVIVLSADG
                     HLKYVNPAALRMYDLPSGRAAAELIKRVANSPCYDADGTRLESAMHPAALAFRSGRSF
                     TKAIYGMDLPGGNRRWLLISGRLLNADDRHSDVLVSFSDITAERDDLDRLVHQATHDP
                     LTALPNRAFVLRRITEALTSAGCRRLRGVLFIDLDDLKVTNDTLGHDAGDDLLNATAM
                     RLREVAGPVNVVGRHGGDEFVVLIYRNVTRDGLEGLVRRLRARLAEPVIIADTTIAIR
                     ASVGFVHVDGDDTRSAEEILRDADRAMYVAKRAGRGLR"
     gene            9836..>10026
                     /locus_tag="VGP27_19115"
     CDS             9836..>10026
                     /locus_tag="VGP27_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7853445.1"
                     /translation="MKRSVLVLASVLMVVAACASSRAQPNGGPEDIATSLAVPWGIAF
                     LGDGSALIAERDSAAVKRLA"
BASE COUNT         1666 a         3215 c         3490 g         1655 t
ORIGIN      
        1 acggtctgca cgtagcgcgg cgtcgcgccg gcgatcgtga acgtgctgat gagcaactcg
       61 ttgaaatagc gtgcctgcac cggcgagtac atcaccacgt cctggccgtc gaggtcgttc
      121 aacgtcagtc ctcgccccag cccgacaagc ggatgcgcct cggggagtgc ggcgatcaat
      181 tgctcgtgta acagcggacg cgacaccagg cccggtcgcc tgatcggcgg gcgcgccatg
      241 cccagatcga cctcgccggt catcaaccct tcgagctgaa ccgccgtcac catctcccgc
      301 agatccagct taacgtccgg aagcttctcc cgggccgcgg ccagcagccg tggcaacacc
      361 acgtgcgcgg aggccgccgt gaatccgatg acgacggtgc cgagatcccc ggccggaatc
      421 cgcttcaccg tctgcgcggc gccctcggcg agttggagga tgcggcgggc gtcgggcagg
      481 aacgccaccc cggcgggcgt cagggtgacc gcccgcgtgc tgcgctcgat gagctggaca
      541 ccgagctcgc tttcgagctg ctggatctgg cgactcaacg gcggctgtgt catgtgtagc
      601 cgctctgcgg ccctcccgaa gtgcagctcc tcggcgactg cgacgaaaca ggacagcctg
      661 gcgagtgata acaccgatgc actcctagca tcactgggcc attcgcgttg cgaatggtgc
      721 atcagtttag acctgtgtgg cagctgaatc gggcacccgg ggtatggccc aagcggtaac
      781 tttggctaag agcgtcgaga agggatgtgc gttggtagct gccgaacatc ggcaactctc
      841 tgaggggtta accggggagg gctaccgccg actggtcgag cgcagtcccg acggcatttg
      901 tgtccatgcg aacggacggg tggttttcgt caacgacgcc ggtgtgcggt tgatgctcgc
      961 tgattccagc gaccagatcg tcagccgtcc catcaccgac ttcgtcacac gcgaatcgat
     1021 acctccgatg caatccggcg tcgcccgact ccgtgagctc ggcgactgca ccccgcacta
     1081 cccggctcag atggtccggg tcgacggcag tctgctggcc gtcgagattg tcatcgcgat
     1141 gacgatctgg gaggacgaac ccgcccatca aatcatcact cgagacgtca gcgcgcggca
     1201 tgcctccgag tcagcgctcc ggtaccaagc ggccctcgtc aaccacgtca gcgatgccat
     1261 catcgggaca acggcgaccg gtgtggtgac aagttggaac cccgccgcgg agcagatcta
     1321 tggcaggccg ttcgccgagg ccaagggcca tccgatgagt gagttggtcg gcgctgagct
     1381 cgatccgacc gcggtcgtgg cctccggtgg cgtcgtacac agcacccacc gctcggccga
     1441 cggcgcgccg ctcgatgtcc gtgtttcggc cgccgcgatg aacgacggat acgtcttcgt
     1501 gtgctgcgac ctgacggctc tgcatcgcgc ggaacaacac ttcaaggcga tcgtggcgtc
     1561 gatggtcgag ggcgtcgtcc tcatcggcaa ggaccggcgc atcaagtcga tcaacccggc
     1621 cgccatcaac atcatgggtg cgggaccgga atacgtcggt agcgacttct tcgcgatcac
     1681 ggatcagttt cactttctcg acgccgacgg cgtgaacatc ccgcccgctc ggcgcccagt
     1741 tcgcgccgtg ctacgaaccg gtgtgccgtt cttcaatcag gtgttcggct tcgaccagtt
     1801 cggcggtgaa cgggtatggc tgatgtgcag ctgccgtctg ctcaacccgg agatcctggg
     1861 ccagtccgac atgttgatgt cgttcgtcga cgtcaccgcc gaacgtaaga ccgccgacaa
     1921 aatcctgttt tacgccacgc atgacgcact gaccgaattg ccgaaccggg tttcggtcct
     1981 tcgcagactc aagaaggcgc tcgcggcacc ggagccgggc gatcagttgc gcgcggtgct
     2041 gttcatggac atcgacgacc tcaagtcgac gaacgacacc ttggggcaca cggcgggcga
     2101 cgaccttctc cgcgtcgccg cgggctgtct tcgccgggtc gtcgcggccg acgatgtggt
     2161 cggacggctc ggcggcgacg agttcgtcgt gctggtggca cgcgacgttg ccgaaggtga
     2221 actcgacgac atggtcaacc ggttgcgcat cgaattgaac acgccggctc tgatcggtgg
     2281 gacaagcaca ccgatcggcg ccagcatcgg cgtcgtcgag gtgcatcgcg acgagaagcg
     2341 cacgcccgac gagatactgc gcgacgccga cctcgcgatg tatgaggcca agcgggcgcg
     2401 ccgccgggac gtgtagccga acccgtcgct accagcgcgg gctgctcgcc tcgaaaccgg
     2461 ggtcgaccgt ctgcatgtaa ccggtgtcgt cgcggtcgcg gataccgcag cgcaggtact
     2521 gctcgtgcag agcgccgagc gcgtccacgt cgagttcgac gccgaggcct ggtgtcgacg
     2581 gcacggccac cgcgccgtcg aggaactcca gcgcacccgg cttgacgatc tcctcagtct
     2641 tccacggcca gtgggtgtcg caggcatagg tgagattcgg cgtggcgcct gccaggtgca
     2701 ccatcgcggc aaggctgatg cccatgtggg agttggagtg catcgaaagc ccgaggccga
     2761 acgcctcgca aatgccggcc agcaggcgtg agcgctgcag gccaccccag tagtggtggt
     2821 ccgaaagcac gacgtgcacc gagtttctgg cgaccgcagg agcgaggtgg tcgaacgcga
     2881 cgacgcacat gttggtcgcc agcggcatcg gagcctgcgc agcgacctcg gccatcccgt
     2941 ccagcccggg cgttggatcc tcgaggtact cgaggatgcc tgccaagccg gacgcgacct
     3001 tgaccgacgt ctgaggcgtc caggcagcgt tggggtccag ccgcagcggg tggttgggga
     3061 aggccgcgtg cagggcctcg atcgccgcca cctcctcctc gggcggaaac acgccgccct
     3121 tgagtttgat tgcggtgaag ccgtattcgt cgacgatgcg cttggcctgc gcgacgaggc
     3181 cgtcggggtc gagcgcctcg ccgtaagcgt cgggctcagc gcctgggtgc gccgcccact
     3241 tgtagaagag gtaggcgctg aacggcaccg cgtcgcggac cttgccgccg agcagatcgg
     3301 agacgggcct gccggtggcg tggccctgca cgtcgaggca cgcgacttcg aacggtgaca
     3361 gcacctggtc gacggcgctg gccgtggtga tcatgcccgc cgtcccgaca gcgcgcgagt
     3421 cgccggtcag cctgtcggcg atggcggcgc gaatcgcgtt gagcccgaag acgtccagtc
     3481 cggtgatggc gtcagcggcc gctcggagcc ggtccaaatg agtctcgtcg gcgtaggtct
     3541 cgccgaggcc gacgagacca gcgtcggtat cgagttggat gatcgcccgc agcgcgaacg
     3601 gctggtgcac cccgacggtg ttcagcaggg gcgggtcacc gaacgcgatc ggcgtgatgc
     3661 gggcgcccgt gatgcggatc gggcccggcg ccatcgtcag cgcgcctcgg cgagcgcatc
     3721 ggcgaggacc gcgcgtccgg cggcgatgat cgagcgcagt tccgcgaggt cgtcggcgct
     3781 cggcatcacc agtggcggcc ggaccgggcc tgcgggcgtg ccctcgaggg tgacgccggc
     3841 cttgaccagc gagacggcgt agccgggcac ggtgtcgcgc aaccggacca gtgggtggaa
     3901 gaacgctcgc gacaacgcgt cgatgagcac ctcgttgccg gtctcgagcg cgttgtagaa
     3961 cgcgagagcg atgtcggcag cgaaggcgaa cgtggccgag gagtacagcg gaacaccgat
     4021 cgcgcggtag gcctgctgag tggtctcggc ggtgggcatg ccgttgaaga acaggaagtc
     4081 cttggcctca ccctccagcg cgtctgtgac cgcgcgaacg atacgcgcga ctttatcgag
     4141 atcgcctgtg ccgtccttga atccgatgac attcgggatc ttcgacaccg cgatcgcaga
     4201 cgcttcggtg aagcgggcgt tgttgcggtt gtagacgatg acgggcaggt cggtggcggc
     4261 ggctacatcg cggacgaagg cgacgaggcc ggcctgtggc atttcgacca ggtacggcgg
     4321 cagcagcagc aacccgtcgg ctccggcatc cttggcagcc ttggcgaact gcttggcctg
     4381 cgcgatcgag ccgcctgcgc ccgcgtaaac cggcacgcgg ccggcgacca cctcgacggc
     4441 ggtgcggacg acgggctcga actcctgagg gtcaagggca tggaattcgc ctgtgccgca
     4501 ggcgatgaag acaccaccgg gacctgcgtc cacgccgttg gcgatgtgcc gggccagcgc
     4561 gtcgaggtcg acggcgccgc tctcggtgaa cggcgtgaca gggaagaaca gaactccggc
     4621 aaaaacgggg cgtgcggcgc tcaaaacgac tccttgatga gtgggtgggt cagtcctgtt
     4681 ggagccggcc gtcgatgcgc cgccacaggt tatcgggatt gccatcagcg attacgctgg
     4741 gcagcaacga cttcggcatg tcctggtagc agacgggccg cagatagcgt tcgatcgcgc
     4801 gcgctcccac agatgtggtg cgcgaatccg acgtcgcggg cgaggggccg ccgtgcacca
     4861 tggcgtggca gacctcgacg ccggtgggcc agccgtcgaa caggatccgg cctgccttga
     4921 gctccagcag cggcagaatc tgccgcacct cgtcgtaatc ggactcgtcg gcgtgcaccg
     4981 tcgcggtgag ctggccttcg atgtgctcgg cgacgctccg catctcggcg ggatccgagc
     5041 agcggacgat caggctggag gatccgaaga cctcgccctg cagcgtcttc gagctcagga
     5101 aggttttcac gtcggtagcg aacagtgctg cccgacagcc ggtttggctg tcgccggctt
     5161 cgccgcggcc gatcaggtcg gcttccgcgc tgagttcggt gacgccgttg gtgtacgcgt
     5221 cggcgatgcc gggagtgagc atgggtgtag ccgatgactg cgccagcgca tcacgcgccg
     5281 ctgtgacgaa cgtgtcgagc cccttgccgt cgagggcaat gaccagaccg ggatttgtgc
     5341 agaactgtcc ggagcccagt gtcagcgagt tgacgaacgc gcgacccaga tcggcgccgt
     5401 ggccggccag cgctccgtcg agcaggaaca cggggttgat cgagctcatc tcggcgtaga
     5461 cggggatcgg ctcgggacgg ccggccgcgg cggcgacgag tgccattcca gcggaacgtg
     5521 atccggtgaa gccgaccgcc ttgattcgcg ggtcggtgac cagggtgacg ccgagttcgg
     5581 ggccgaatcc gtacagcagc gagaatgtgc cggccggcag acccgcggcg gcgacggcgt
     5641 cggagatggc gcggccgacg agctccgacg tgcccggatg cgcgtcgtgc gccttgacga
     5701 cgacggggca cccggcggcg agcgccgacg cggtgtcccc gcctgcgacc gagaacgcca
     5761 gtggaaagtt gctggcgccg aatacggcga ccgggccgag cgggatggag cgctgccgaa
     5821 tgtcgggtcg tggcaacggg gttcgatcgg gcagtgcggg gtcgatgcgg gcggcgttcc
     5881 agctgccctc gcgaaggacg ccggcaaata ggcggagctg tccggtcgtg cggccgacct
     5941 cgccgttgag gcgggcctgc ggcagtccgc tttcggcgac ggcgcgctcg acaagagcgt
     6001 ccttgacggt ctcgatgttg gcggcgatcg tttcgaggaa ctgggcgcgc tgttcggatg
     6061 tcgtgcagcg gtactcggcg aaggcctcgg cggcggccgc gctggcggcg tcgacatcag
     6121 aggcgccacc gtaggagtaa gcgggttcga gctcgtggcc tgcggtggga tcgaaggcgc
     6181 ggatctgctt accggaaccg cgaacggatt cgccggcaat gatcatctgt cccgtcagat
     6241 gggtctcggg catcgtggca gtcatggttt cctcggcgta agggcgtgca gaaggtccgg
     6301 caagtggtga tgtggctcaa cggtagaagc gacttcgatg cttgtccaag accaataagc
     6361 gcacgactga ttcacgattt agatcaatca accacctggt gcgagcgcct ggctatctcg
     6421 tcgatgtcca tcgacaggcc gcttcggtag tgtcagaccc ggatgacagg cagagggaga
     6481 tagctaatgg acctggtgct cggtctgtcg gtgacctcga cagccgtccg gtgggtgctc
     6541 gtcgaaggcg tgacaggcga cggcgacgcc gtcgaccgcg gagtgcttga tgtcgtcgac
     6601 gcttcgactt tcgatgctga ggccctgctc acggtgttgc tggacgccaa cgaggcggcg
     6661 gtggagagcg gtgtgcacgc cgtcggtgtc acgtggactc ctgctgggga ggccgtcgcg
     6721 ggcgcgatcc tcgaggcgtt gcaggcccgt ggcctgtcga acgccatcgc ggtttcggag
     6781 gtagaggccg ccgaggcact ggcgagcggc atcgccgaca tcgcgggcta tgaaagcgtc
     6841 gcggtttgcg tcttcgagcc cgactacacg ttggtcgcca tggtgaacgc cgacggagtg
     6901 agcgccgacc cccagaatct ccccgacgac gtcgagttgg cgggcagcgt catcgcgctg
     6961 gacctcaacg atcgacagct ggacgccgtc ttcgtcgtcg gctctgcaaa tctcgagccc
     7021 gtcgtgtccg cgatggacgc ggtcgcggcg gccccggtga tctcgtccgc cgaggccgat
     7081 atggcgatgg cccggggcgc cgcggtggca tccgccatgg cggtgaactc catggacggc
     7141 ggctcgacgt cgctgctggg gctgcgcggc atgtcgagaa cgaccgtgtt gacgtccgtc
     7201 gtcgtcgctg cggcagccac actggtgatc tcgctgtcgg tcgtgctggg tctgcaggcg
     7261 acgtcggatt cgccgtctga gaccaagaac gcaaacgcca ccggcgagca ggtgaaagag
     7321 ccgaccgtgc cgacggaggc gaaggccgcg cttgccgcgc ccaagctgca gaagcgcccg
     7381 gcggccgctc cggcgccgaa acccgcaccc gcggcagtcc cgcccgcgcc ggagcctgca
     7441 gctccgccga tcgtgcagac gatggtggcc gcgccgccgg cgcccgaggc ggtgccggat
     7501 gccggtccgc ctgctgcaaa cttgccggac acctgggtgc cgccggccgc gacgccgccc
     7561 gttccggcgt atgcgccgcc tgttccggcc tacgttccgc ccgcgcaacc gcaggttcct
     7621 gccgtgcagc cggaaccgcg tctgcgcgac aagatcatcg agcgcattcc gatcatcaac
     7681 cgattccacg acccgcagcc gatgtacccg acgaactagc tgggcaccac ctccgtacat
     7741 ggcgccgggg tgatccgtgt gagcatcatg cccaccccaa ccacttcgca agcggttgaa
     7801 acatcgcggc tcagctgtgc tcgaagtgct gatagtcctt cggtgtccgc cagtcgccgc
     7861 cccagcgcca accgcggtcg gtgaacgcgc gaacaacggg gtcgcccgca tgcagcagcc
     7921 ccggatcggt gcggttgcgg tcgaggtacg gtcctgcggt cttcggctcg agtgtgcctt
     7981 cccggtcgac gaacgggttg aggagcgggt tgatgtcgat cgcccggccg taggcatgca
     8041 atgcccatgc ccgacctgac ggcatcagtc ggcagttgta ggccgaggtg ttgttgtcct
     8101 ccatcgacag ctcatcctcg gcgccggggt aatgatcgac ggtccgcatc gtcgcgatcg
     8161 gatatcgcaa cacctgcagc tgctcgaaga tcgcgatgac atctgcgacc acgtcctggt
     8221 gcacgacgag ctcgccgcgg tgtgtctgcc cgtcgaagcc gacgtagtcg agattgaccc
     8281 gacgcagcag ttcggggccg acagggcagc cgggtcgcca ggtggcaccg agttcggcgg
     8341 cggtgacggg atggacggcc gcaggtgcga acgccggcga cgcgctggtc ggcgatgacg
     8401 gcggcggcgc tgaaggtgtc ggcggcgtcg tcgtcgtcgc agcgggcggt gcggacgaga
     8461 ccggtgcggt cggtggagtg cgcccgcatt ggagcatgcc ggcagccatg gtggtcagca
     8521 ccgccgcagt ggcgatcggt cgatatcccc taatgacctc acgataacga tggtcgcagc
     8581 cgggctgtcc tcgcaaatat ctagatcgtt gccggaattg cagatctagg accacggggt
     8641 tccgcgaagc tttggcgttg ctcagcgctg accggatacg cggggctcgc catgagggca
     8701 aacgtcaatt atggtggtgc catgcatgct caaacagccg ggcgcgccga aaaacgcacc
     8761 ggatcgtccg caacactgga aattatgctg atccgccaaa gagcaaactc tttcgcgagg
     8821 gacggcgcgg gcacctgaca tgggggagcg cgacaccgcc caccagcccg ccacccgcga
     8881 cgtcaccgcg ctgaccggcg tcaaggaacc ggctttcgaa gcgatcgttc acatgctcga
     8941 cgacggagtg atcgtgctga gcgcagacgg ccacctcaag tacgtcaatc ccgcggccct
     9001 gcgcatgtac gacttaccgt ccggacgcgc cgctgccgag ctcatcaagc gggtggcgaa
     9061 ttccccgtgc tacgacgccg acgggacccg gttggagtcc gccatgcatc cggcggcgct
     9121 ggcattccgc tccggccggt cgttcaccaa agcgatctac ggcatggatc tacccggcgg
     9181 gaatcggcgc tggctgctca tcagcgggcg cctgttgaac gccgacgacc gacactcgga
     9241 tgtgctggtg tcgttctccg acatcaccgc cgagcgcgat gatctcgatc gcctggtgca
     9301 ccaggcaact cacgatccat tgaccgcgct ccccaaccgg gcattcgtgc tccgcaggat
     9361 cactgaggcg ttgacgtcgg ccggctgccg acggttgcgt ggcgtgctgt tcatcgacct
     9421 ggacgacctc aaggtcacaa acgacacctt gggtcatgac gcgggcgacg acctcctgaa
     9481 cgccactgcg atgcgactgc gggaggttgc cggcccggtc aacgtcgtcg ggcgacacgg
     9541 tggtgacgag ttcgtcgttc tgatctatcg gaatgtaacg cgggacgggc tcgagggcct
     9601 ggtgcgccgg ctgagagccc gactggcaga gccggtgatc atcgcagaca ccaccattgc
     9661 gattcgtgcg agcgtcggct tcgtgcatgt tgacggcgac gacacgcgaa gtgccgaaga
     9721 gatcctgcgc gatgccgacc gggcgatgta cgtggctaag cgcgccggcc gcggcctgcg
     9781 gtgacccctg gcgttgccct ttttccgcgc tccacaacgg gggtataggg acgtcatgaa
     9841 acggtccgtc ctcgttctgg cgtcggttct gatggtggtc gccgcctgcg ccagttcgcg
     9901 cgcgcagccg aacggcggcc cggaggacat cgccacgagc ctcgccgtgc cgtggggcat
     9961 cgccttcctg ggcgacggca gcgcgctgat cgccgaacgc gacagcgccg cggtcaagcg
    10021 cctcgc
//
LOCUS       DASYLX010000818         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2787769,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000818 DASYLX010000000
VERSION     DASYLX010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787769"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..642)
                     /locus_tag="VGP27_19120"
     CDS             complement(34..642)
                     /locus_tag="VGP27_19120"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV7853446.1"
                     /translation="MTAKLPVDPVKAARRLLGAALTGRGVSAMIVEVEAYGGPPDGPW
                     PDAASHSFRGHGGRNTVMFGPAGRLYTYRSHGIHVCANVVCGSDGVAGAVLLRAAAVE
                     SGLAVAMSRRGESVRPAALARGPGNLCSAFGIVMDDNGIDLFDPHSPVQLTLGDERQW
                     ADGARVGVSQAADRPWRFWLAGRPEVSLYRRSPRAPAPGESD"
     gene            702..1466
                     /locus_tag="VGP27_19125"
     CDS             702..1466
                     /locus_tag="VGP27_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853447.1"
                     /translation="MMTSSSDELLRRGVEPVVVIDHLRVVRGKRPAIHDLSVRIARGT
                     ITGLLGPSGCGKTTLMRSIVGTQLITGGSVTVLGQPAGSAPLRKRVGYMPQAATIYDD
                     LRVVDNVRYFGSLYGMDATAADEAVSAVGLDDHRTALCGNLSGGQRTRVSLACALVAA
                     PEVLVLDEPTVGLDPVLRVDLWQQFHQLARRGTTLLVSSHVMDEADHCGDLLLMREGH
                     LLAHTTPGKLREDTGCQSLEEAFLSVIRHSTATAAS"
     gene            1400..2218
                     /locus_tag="VGP27_19130"
     CDS             1400..2218
                     /locus_tag="VGP27_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853448.1"
                     /translation="MSITGGSVSVRHPAQHRNRSKLSPQSYFATTARILRQLAADHRS
                     VAMILVVPSLIITLMYFMFQNVPHRPGAPSPFNNACLIMLGVFPLIVMFLITSITMQR
                     ERVSGTLERILTTPLRRLDLLAAYGTAFSIAAAAQATLACVVSFWLLGLDTKGSPILV
                     FVIAIINAVLGVGLGLLCSAFARTEFQAVQFMPVVIAPQLLLCGIIVPREMLPDWLQW
                     ISNALPASYALEALQQVGAYPDLTAIALRDIIVVIGFAVLALCLAAATLRRRTP"
     gene            2223..2837
                     /locus_tag="VGP27_19135"
     CDS             2223..2837
                     /locus_tag="VGP27_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7853449.1"
                     /translation="MTTNTERKRPGRPPGTSDTRERILNSARELFARNGIDKTSIRAI
                     AGDAGVDPALVHHYFGTKTQLFAAAIHIPIDPMAVIAPLREIPVERIGHVLPSILLPL
                     WDSEMGKGFIATLRSILAGNDVSLVRSFLQEVITAEVGSRVDDPPGSGRIRVQFVASQ
                     LVGVVMARYILELDPFRSLPVDQIAETIGPNLQHYLTGDLPGLG"
     gene            complement(2896..3105)
                     /locus_tag="VGP27_19140"
     CDS             complement(2896..3105)
                     /locus_tag="VGP27_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEV7853450.1"
                     /translation="MVDEKLLSILVCPEDRGSLLLIGDDEMLYNPRLRRAYRIEDGIP
                     VLLVDEAVAVTDDAEHERLLGRAGS"
     gene            complement(3116..3595)
                     /locus_tag="VGP27_19145"
     CDS             complement(3116..3595)
                     /locus_tag="VGP27_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853451.1"
                     /translation="MTSQNAWRRVAGAVAGGLIAGALIAGIGAPTAFAAPADDDTTGT
                     DAPRMTADQALAIIKQDYDTGAGGGQISNFVHEVLVLRNQGYRPSKGNQDAIIAALDK
                     RPNQAPLIDALKATIAYQRRLQAQADAQQQQGNPGFAIGGAPAPFDGGGAPVNLPLG"
     gene            complement(3686..6649)
                     /locus_tag="VGP27_19150"
     CDS             complement(3686..6649)
                     /locus_tag="VGP27_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7853452.1"
                     /translation="MDLSAITRPVGRLVATAQNGLEVLRYGGLETGAVPSPFQVVESV
                     PMYRLRRYFPPDTRPGAKPPGPPVLMVHPMMMSADMWDVTRDEGAVGILHTAGIDPWV
                     IDFGEPDKVEGGMQRNLADHVVALSEAIDTVKETTERDVHLAGYSQGGMFCYQTAAYR
                     RSKDLASIIAFGSPVDTLAALPMNLPAGLAAGAADFMADHVFSRIDIPSWLARTGFQM
                     LDPIKTAQSRLDFLRQLHDREALLPREQQRRFLASEGWIAWSGPAIAELLKQFIAHNR
                     MMTGGFSIHGDLVTLNDINCPVLAVVGEVDDIGQPASVRGIKRAAPQADVYEYLIRAG
                     HFGLVVGSKAATQTWPTVALWVKSIDGDADLPEGVAPMALQPAQHAETGVPLASRLAH
                     GATAAGEMAFTLARSAADAVVAANKSARALAVETVRTLPRLARLGQINDHTRISLGRI
                     MSEQARDVPNGEALLFDGRVHTYEAVDRRINNVVRGLIAVGVRQGAHVGVLMETRPSA
                     LVAIAALSRLGAVAVMIPPDADLATVVRLGAVSDIIADPSNLNAARGLSMRVLVLGGG
                     ESRDLHLPDDADVVDMEKIDPDEVDMPGWYRPNPGLARDLAFIAFSTVGGQLLARQIT
                     NYRWALSAFGTASAANLGRNDTVYCLTPLHHQSGLLVSLGGAVVGGARIALSRGLVSD
                     RFVQEIRQYGVTVVSYTWAMLREVIDDPSFALTGNHPVRLFIGSGMPAGLWKRVVDIF
                     APARVVEFFATTDGQAVLANVSGAKVGSKGRALPGGGEVELAAYDAEDDLILEDDRGF
                     VRKAGTNEVGVMLAHPRGPIDPTASVKRGVFAPADTWVSTEYLFRRDQDGDYWLVDNR
                     SLVIRTERGPVYAMPVNDALGAIDAVDMSVTYAIESGGQRVAVTAVALRPGGSVAAGD
                     LSEALADLPVGVAPDIVHVVPEMALSASYRPLISPLRAMGIPKPSRNAWYLDSDTDRY
                     KRLTAAVRNEIVGGE"
     gene            6757..>6912
                     /locus_tag="VGP27_19155"
     CDS             6757..>6912
                     /locus_tag="VGP27_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853453.1"
                     /translation="MANLINVERATLGYGTRTLLDAVSLGVDEGDAIGVVGRNGDGKS
                     TLLQVLTG"
BASE COUNT         1147 a         2359 c         2222 g         1184 t
ORIGIN      
        1 gttcgacgtt cccatgatct cgatcttccc gtactagtcg ctttcacccg gcgccggtgc
       61 ccgcggactg cggcggtaga gcgagacttc cggccgtccc gccagccaga accgccatgg
      121 tcgatcggcg gcctggctga ctcccacgcg cgcaccgtca gcccactggc gttcatcgcc
      181 cagcgtcagc tgcactggac tgtggggatc gaagaggtcg attccgttgt cgtccatgac
      241 gattccgaat gccgagcaca aattgcctgg accacgggcc aaggccgccg gccgaaccga
      301 ttcgccgcgt cgactcatcg ccaccgcgag gcctgactca actgccgcgg ctcgcagcaa
      361 taccgctccg gcgactccgt cggagccgca gaccacattg gcacatacgt ggatgccgtg
      421 gctgcgatag gtgtagagcc gacccgccgg tccgaacatg acggtgttgc gcccgccatg
      481 tccgcggaag gaatgggaag cggcgtccgg ccagggaccg tcaggcgggc cgccataagc
      541 ctcgacctcg acgatcatcg cgctcacccc gcggccggtc agcgccgcgc ccagaagcct
      601 gcgcgccgcc ttcacggggt cgacaggcag cttcgcggtc atcggggcga ttctgccctc
      661 cgttcttgac acgcccaact gcagagagca ctattcatca tatgatgact tcatcgagcg
      721 atgaattatt gaggcgaggc gtcgagccgg tcgttgtcat cgaccacctg cgcgtcgtgc
      781 gcgggaaacg ccccgccatc catgacttgt ccgtgcgaat cgcccgcggc accatcaccg
      841 gcttgctcgg gccctctggc tgcggcaaga ccacgctgat gcgcagcatc gtcggaacgc
      901 aactcatcac cgggggcagc gtcacggtgc tcgggcaacc ggccggctcg gctcccctgc
      961 gcaagcgcgt cgggtacatg ccccaggcgg caactatcta cgacgatctg cgcgtcgtcg
     1021 acaacgtccg atacttcggc tcgctttacg gcatggacgc caccgccgcg gacgaggccg
     1081 tgtccgccgt aggcctcgac gaccatcgga ccgccttgtg cggcaatctg tccggtggtc
     1141 agcggactcg cgtatcgctt gcctgcgcat tggtggcggc accggaagtg ctcgtgctgg
     1201 acgaacccac ggtcggcctt gaccccgtcc tgcgggtgga cctgtggcag cagtttcacc
     1261 aactcgcccg gcgcggaacg acattgctcg tttcgagtca cgtcatggac gaggccgatc
     1321 actgtggcga cctactgctc atgcgagaag ggcaccttct cgctcacacc acaccaggaa
     1381 aactacggga ggacacggga tgtcaatcac tggaggaagc gtttctgtcc gtcatccggc
     1441 acagcaccgc aaccgcagca agctgagtcc ccagagctac ttcgccacca cggcgcgaat
     1501 tctgcgccaa ctcgccgcgg accaccgcag tgtggcgatg attctcgtgg tgccgagcct
     1561 catcatcaca ctgatgtact tcatgttcca gaacgtaccg catcgaccgg gcgcgccttc
     1621 gccgttcaac aacgcctgcc tgatcatgct cggcgtgttc ccgctgatcg tcatgtttct
     1681 gatcacctcg ataaccatgc agcgggaacg ggtttcggga acgttggagc ggatcttgac
     1741 caccccgctg cgtcgactcg acctgctcgc cgcctacggc accgcgttct cgatcgcagc
     1801 cgctgcgcag gccacgcttg cgtgcgtcgt gtcgttctgg ctcctcgggc tggacacgaa
     1861 aggcagcccg atactggtgt tcgtcatcgc gatcatcaac gcggtgctcg gggtcggcct
     1921 cggcctgctg tgcagcgcat tcgcccgtac cgagttccag gcggtgcagt tcatgcccgt
     1981 ggtcatcgcg ccgcagctgc tgctgtgcgg gatcatcgtg ccccgcgaga tgctgcccga
     2041 ttggctgcaa tggatcagca acgcactgcc ggcgagttac gctcttgagg cgctgcagca
     2101 ggtcggcgct tacccggacc tgacggcaat cgctctgcgg gacatcatcg tggtgatcgg
     2161 cttcgcggtc ctcgccctgt gcctcgccgc cgcgacgcta cgacgaagga caccgtagac
     2221 ctttgacgac caacaccgag cgcaaacgtc ccgggcgccc gcccgggacg tccgacactc
     2281 gcgaacgcat tctgaacagc gctcgggagt tgttcgcccg caacgggatc gataagactt
     2341 cgatccgcgc catcgcaggc gatgcgggtg tggacccggc gctggtgcat cactacttcg
     2401 gcaccaagac gcaattgttc gccgcggcga tccatattcc catcgatccg atggcggtga
     2461 tcgccccgct aagggaaatc cctgtcgaac ggatcggtca cgtgttgccg tcgatcctgt
     2521 tgccgttgtg ggactccgag atgggcaaag gctttatcgc gacgctgcgg tcgatcctcg
     2581 cgggcaacga cgtgtcgctc gtccggtcgt tccttcagga agtgatcact gccgaggtgg
     2641 gctcgcgggt cgacgatccg cccggcagcg gtcggatccg cgtccagttc gtggcctcac
     2701 aactggtcgg cgtggtgatg gcccgctaca tcttggaact cgacccgttc aggtcgttac
     2761 cggtcgacca gatcgcggag acgatcggac cgaacctgca gcactatctc accggtgacc
     2821 ttccggggct tggctagcaa cccaaaggct tcgcgcagaa gtgtgttccc gatcgagacg
     2881 aaacgattac gagtgtcagg accccgcacg gcccagaagc cgctcatgct cggcgtcgtc
     2941 ggtcacagcg accgcctcgt cgaccagcag caccgggatg ccgtcctcga tccggtacgc
     3001 gcggcgcagc ctcgggttgt agagcatttc gtcgtcgccg atcaacagca gcgagccgcg
     3061 gtcttcgggg cagaccagaa tgctcagcag cttttcgtcg accaccacgg tagcctcagc
     3121 cgagcggcag gttgacgggc gcgccgcccc cgtcgaacgg cgcgggtgcg ccgccgatgg
     3181 cgaagccggg attgccctgc tgttgttgtg cgtcggcctg cgcctggagc ctgcgttggt
     3241 aggcgatggt cgccttcagc gcgtcgatca ggggtgcctg gttcggccgc ttgtccagcg
     3301 ccgcgatgat cgcgtcctgg ttacccttcg acggccggta gccctggttg cgcaggacga
     3361 gcacctcgtg gacgaagttg gagatctgac cgccgcctgc acccgtgtcg tagtcctgct
     3421 tgatgatggc aagcgcttgg tcagcggtca ttctcggcgc gtcggtgccg gtcgtgtcgt
     3481 catccgcagg tgcggcgaat gcggtcggcg caccgatacc cgcgatcaga gcgccggcga
     3541 tcaggccgcc ggccacggca cctgccacgc ggcgccaagc gttctgcgat gtcatcgaac
     3601 ctccagtgcg acggctcatc ttgatcgtgc tcctcgcgtg cgcgatggaa gcctaacagt
     3661 gtgccggacg cgcggcacgg tctcgctact cgccaccgac gatctcgttg cgaaccgcgg
     3721 ccgtcagtcg cttgtaccta tcggtatccg aatccaggta ccaggcgtta cgcgaaggct
     3781 tgggaattcc catcgcacgc agcggactga tcagcggtcg atatgacgcg ctgagcgcca
     3841 tctcgggcac cacatgcacg atgtccggcg ccacaccgac cggcagatcg gccaacgctt
     3901 cggacaagtc ccccgccgcg acgctgccgc ccggacgcaa cgccacggcg gtcaccgcga
     3961 cccgttggcc gccggattcg atcgcatagg tcaccgacat gtccacagca tcgatagcgc
     4021 cgagagcgtc gttgaccggc atggcataaa ccggtccacg ctcggtgcgg atgacaaggc
     4081 tgcggttgtc caccaaccag tagtcgccgt cctggtcgcg ccggaacaaa tactcggtgg
     4141 acacccaggt gtcggcaggg gcgaacacac cgcgcttgac cgatgccgtc gggtcgatgg
     4201 gtccgcgcgg atgcgccagc atgacgccca cttcgttcgt tccggccttc cgcacaaagc
     4261 cgcggtcatc ctcgaggatc agatcgtcct cggcgtcgta cgcggcgagt tcgacttcgc
     4321 cgccgcccgg aagtgctctg cccttgctgc ccaccttcgc accggacacg ttggccagta
     4381 cggcctgacc gtcggtggtg gcgaagaatt cgacgacgcg tgccggcgcg aagatgtcga
     4441 ccacgcgctt ccacagcccg gccggcatgc cggatccgat gaacagccgt accggatggt
     4501 tgccggtcag cgcgaacgac gggtcgtcga tgacctcccg cagcatcgcc caggtgtagg
     4561 acaccacggt cacgccgtac tggcggatct cctggacgaa ccggtcggac accagacccc
     4621 gcgacagggc gatgcgcgcg ccgccgacca ccgcgccacc gaggctcacc agaagccccg
     4681 actggtggtg cagcggggtc aggcagtaca cggtgtcgtt acggccgagg ttggccgccg
     4741 acgcggtccc gaacgccgac agcgcccagc ggtaattggt gatctggcgg gcgagcaact
     4801 ggccgccgac cgtgctgaaa gcgatgaacg cgaggtcgcg ggccaacccc gggttgggtc
     4861 ggtaccagcc gggcatgtcc acttcgtcgg ggtcgatctt ctccatgtcc acgacgtctg
     4921 cgtcgtcggg caggtgcagg tcgcgcgatt cgccgccgcc gagcaccagc acccgcatcg
     4981 acagcccgcg ggcagcgttc aggttgctgg ggtcggcgat gatgtcggac accgcgccca
     5041 gccgcacaac cgtggcgagg tcggcgtcgg gcggaatcat cacggccacc gccccgaggc
     5101 gcgacagcgc agcgatcgcc accaacgcgc tgggtcgcgt ctccatcagc acaccgacgt
     5161 gcgctccctg tcggacgccg accgcgatca ggccgcggac cacgttgttg atacggcgat
     5221 cgacggcctc ataggtatgg acacgtccgt cgaacaggag ggcctcgccg ttcggtacgt
     5281 cgcgtgcctg ctcggacatg atgcggccga gcgatatgcg ggtgtggtcg ttgatctggc
     5341 cgagccgcgc cagcctgggc agcgtgcgga ccgtctcgac ggcgagcgcc cgagccgact
     5401 tgttggcagc gacgaccgca tcggcggccg agcgggccag ggtgaacgcc atctcacctg
     5461 ccgcggtggc cccgtgtgcc agccgggagg cgagtgggac tccggtctcc gcgtgctgcg
     5521 ccggctgcag cgccatcggc gctacgcctt cgggcagatc ggcgtcgccg tcgatcgatt
     5581 tgacccagag cgccaccgtc ggccaggtct gggtcgccgc cttcgatccg acaaccagac
     5641 cgaaatggcc tgcgcgaatg agatattcgt agacatcggc ctgcggcgcg gcccgcttga
     5701 tgccgcgcac cgacgcgggc tgaccgatat cgtcgacctc accgacgacc gccagtaccg
     5761 ggcagttgat gtcgttgagc gtcaccagat cgccgtgaat ggagaatccg ccggtcatca
     5821 tcctgttgtg ggcgatgaac tgcttgagca gctcggcgat cgcgggcccc gaccaggcga
     5881 tccagccctc cgatgccaga aaccgccgct gctgctcccg gggcaacagc gcttcgcggt
     5941 cgtgaagctg ccgcaggaag tccagccgcg actgcgcggt cttgatcggg tcgagcatct
     6001 ggaagccggt ccgcgcgagc cagctcggaa tgtcgatgcg gctgaacacg tggtcggcca
     6061 tgaaatccgc ggcaccggcc gcgagtccgg cggggaggtt catcggcaat gcggccagcg
     6121 tgtccaccgg tgaaccgaac gcgatgatgc tcgcaaggtc cttcgaccgg cgatacgccg
     6181 cggtctggta gcagaacatg ccgccttggg agtagccggc cagatgaacg tcgcgctcgg
     6241 tcgtctcctt gacggtgtcg atggcctcgc tcaacgcgac gacgtggtcg gcgaggttgc
     6301 gctgcatacc gccctcgacc ttgtccggtt ccccgaagtc gatgacccat gggtcgatgc
     6361 cggcggtgtg caatatgccg acggcgccct catcgcgggt gacgtcccac atatcggccg
     6421 acatcatcat cgggtgcacc atgagcaccg gcggcccagg cggtttcgcg ccgggccggg
     6481 tgtcgggcgg gaagtagcgg cgcagccggt acatcggcac gctttccacc acctggaagg
     6541 gcgacggcac cgcgcccgtt tcgaggccgc cgtagcgcag cacttcgagg ccgttctgcg
     6601 cggtcgccac caaacggccg accggcctgg ttattgcgga aaggtccacc accggcggtt
     6661 cccctcttgt cacacctgtc ccccgattgc cgaggtcatc atggcacaga gcccgtggca
     6721 ccctcggcaa gatgccctac ctatcatcgg cggctgatgg ccaacttgat caacgtcgaa
     6781 cgcgcgacgc tcggatacgg cacccgcacc ttgctggacg ccgtcagcct cggcgtcgat
     6841 gaaggcgacg cgatcggcgt cgtcggccgt aacggcgacg gcaagtcgac gctgctccag
     6901 gtgctgaccg gt
//
LOCUS       DASYLX010000819         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3665880,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000819 DASYLX010000000
VERSION     DASYLX010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665880"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VGP27_19160"
     CDS             complement(<1..646)
                     /locus_tag="VGP27_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7853454.1"
                     /translation="MKHAPTGPVLSDGTPLSELIDKDFREVSLRVLTDPELYQLELKH
                     LFAKAWTVVAHEDEVPNVDDYVLRYVGEDQVIVSRAADDTFTVALNVCAHRAAKVCRF
                     EAGNAKTFQCAYHGWVYKSDGSFVGAPIAREDMHGLLRPKSELGLTKGRVETYCGMIF
                     ATFDENAPTLREYLGPMTWYWDLMFDRTPGGMTVLGHPQRFIIKANWKSAAEQFA"
     gene            complement(699..1520)
                     /locus_tag="VGP27_19165"
     CDS             complement(699..1520)
                     /locus_tag="VGP27_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853455.1"
                     /translation="MSRLDGKVAVVTGAGKGIGRGIARRFAHEGAKVIIAEFDADAGK
                     RVADDLVELGGEGLFVQTDVSQKADIEGALAAAQESFGGLDILVNNAISLTPDVPLEY
                     KTDEMLEQTMKVGLWSVWWGMQTAFPVMKAQGGGRIINFYSIDADNGNWVHGDYNTAK
                     GGVQALTRTAGFQWARHNICVNAIAPIAASAAFEQMVADNPALGEAIPTMIPMGRMGD
                     PEDDIAPVAAFLASEEARFITGATIPVDGGLHVPRLNTRPPDPSVFDSMSGSPGQ"
     gene            complement(1520..2086)
                     /locus_tag="VGP27_19170"
     CDS             complement(1520..2086)
                     /locus_tag="VGP27_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7853456.1"
                     /translation="MPAITDKFTGEPVLKLQRLGHGTLETVDIARARRFYEAVLGLEV
                     IQPSSRSMMIRLGTNHAYAVVETGKESTMSMLAHNGLDVGSVEEVHAAYEKLVAIKDE
                     WGIKQITEPRHMHGDTSFYFTDPDGNWWEIVAVRENGYAEDFTDPERDLTGLHEFDGE
                     GGNVNFNHTHDPDFRARVREVVNAKSDA"
     gene            complement(2123..2530)
                     /locus_tag="VGP27_19175"
     CDS             complement(2123..2530)
                     /locus_tag="VGP27_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853457.1"
                     /translation="MTDNHHDVLSTVDKFVEALSTGDAAGLDALFTDDAVIWHNYDQV
                     GQPAREALAALAGLGALQPRYEIAGRDVVDGACIQRHVVHVTLPGGQSASIPAIQRIA
                     MTDGRIRRIDEYMDSAQMAAAVQAMQASPQAGV"
     gene            complement(2558..3016)
                     /locus_tag="VGP27_19180"
     CDS             complement(2558..3016)
                     /locus_tag="VGP27_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853458.1"
                     /translation="MTTATTAATKTMQAMTVHWELVRLNNAFAFYLDNGVFESMIELF
                     TPDGLFDRAGIVHRGQDEIRDGMAERPKVTTRHLLTNFHLTSLDDDAAEAVVCAMVYH
                     GPVAENGEAVVYATDNGRVIEFHDRYVKTVDGWRIASRIARPIFTPKVWP"
     gene            complement(3026..4213)
                     /locus_tag="VGP27_19185"
     CDS             complement(3026..4213)
                     /locus_tag="VGP27_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853459.1"
                     /translation="MTAIDADTATTTVLDAVRALAPEIAERAGEIEAAKVVPADLIER
                     LTAAGAFRMFVPRQYGGEEMSLAAAMEVIEEVARADASTGWTVMIGADFAPVFGRFPQ
                     HVLDGQIYPDGPDTMARGAFAPKGVAIRTDGGYLVNGQWPLASGSYEHQWMIGSCVVL
                     RNGAPIMTDAGVPEMVLAMVPARDAQFVQTWDSVGLCGTNSNDFVLKDVFVSNDHTAQ
                     FFGPATVDTPLFRLPVRLALGPTHVAVVMGIAEGALADLRELAKTKRPAFNPTQRLAA
                     DPVFQYRLGQLEIRLAAVRALAEKEVNLMWDAALSREPISPLAATRQRAMVARAHTEC
                     VEIVNEAFGLAGSNAMYNTSTLQRRLRDVRTAAQHAAASAEIYTIVGALLVDEDVPPA
                     ALG"
     gene            complement(4359..>4592)
                     /locus_tag="VGP27_19190"
     CDS             complement(4359..>4592)
                     /locus_tag="VGP27_19190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7853460.1"
                     /translation="WMPTGTGAVMIGGASAWLECALFDEVPAGDHTLAILQIHRLCTH
                     PAVAPLVFHAGRFRRLEADAPAIAPGGHGQRSR"
BASE COUNT          745 a         1499 c         1472 g          876 t
ORIGIN      
        1 cggcgaactg ctctgcggcg ctcttccagt tggccttgat gatgaaccgc tggggatggc
       61 cgagtaccgt catgccaccc ggcgtgcggt cgaacatcag gtcccagtac caggtcatcg
      121 gaccgaggta ctcacgcaga gtcggggcgt tctcgtcgaa ggtcgcgaag atcatgccgc
      181 aataggtctc cacccgaccc ttcgtcaggc caagctcgga cttcggccgt aacagtccgt
      241 gcatgtcctc ccgagcgatg ggtgcgccga cgaagctacc gtcggacttg taaacccaac
      301 cgtggtaggc acattggaac gtcttggcgt tgccggcctc gaaccggcac actttggcgg
      361 cccggtgcgc acacacgttg agcgccaccg tgaaggtgtc gtctgcggcc cggctgacga
      421 tcacctggtc ctcgccgaca taccgcagca cgtagtcgtc gacattcggg acttcgtcct
      481 cgtgtgccac caccgtccac gccttggcga acaggtgctt gagctccagc tggtaaagct
      541 cggggtcggt caaaacccga agcgagacct cccggaagtc tttgtctatc agctccgaca
      601 gcggggtgcc gtctgacagg acaggtcccg tcggagcgtg tttcacctca tctatggtca
      661 cgccgaaatc tcctgttcct ctaaccgata ccttgttgtc attgcccggg actgccggac
      721 attgagtcga agaccgaagg gtccggcggc cgggtgttca gccgcggcac gtgcagaccg
      781 ccgtcaaccg ggatggtggc cccggtgatg aagcgggcct cctcgctggc cagaaatgcg
      841 gccaccgggg cgatgtcgtc ctccggatcg cccatccgtc ccatcgggat catcgtcgga
      901 atcgcctctc ccagtgcggg attgtcggca accatctgtt cgaaggcagc cgatgcagcg
      961 atcggagcaa tggcgttgac acagatgttg tgccgtgccc attggaaacc tgcggtccgg
     1021 gtcagcgcct gcacgccgcc ctttgcggtg ttgtagtcac cgtgcaccca gttgccgttg
     1081 tcggcgtcga tcgaatagaa gttgatgatc cggccaccgc cttgggcctt catgacgggg
     1141 aacgcggtct gcatgcccca ccagacgctc cacagcccga ctttcatggt ctgctcgagc
     1201 atttcgtcgg tcttgtattc cagcgggacg tccggggtca acgagatggc gttgttcacc
     1261 agaatgtcca ggccgccgaa gctctcctgc gcggccgcca gtgcgccctc gatgtccgcc
     1321 ttctgcgaca cgtcggtctg cacgaacagt ccttcaccac cgagttcgac cagatcgtcg
     1381 gcgacccgct tccccgcgtc ggcatcgaac tcggcgatga tgaccttggc gccctcatgg
     1441 gcgaagcggc gggcgatccc ccggccgatg cccttgcccg ccccggtgac gacggcgacc
     1501 tttccgtcga gacggctcat tacgcgtcgc tcttcgcgtt cacgacctcg cgcacccggg
     1561 cacggaagtc gggatcgtgc gtgtggttga agttcacgtt cccgccttcg ccatcaaact
     1621 cgtgcaggcc ggtgagatcg cgctcgggat cggtgaaatc ctccgcatag ccgttctcgc
     1681 gcaccgccac gatctcccac cagttgccgt cgggatcggt gaagtagaag gacgtgtcgc
     1741 cgtgcatgtg ccgaggctcg gtgatctgct tgatgcccca ctcgtccttg atcgcgacca
     1801 gcttctcgta ggcggcgtgg acctcctcga cgctgccgac atcgagtccg ttgtgcgcca
     1861 gcatcgacat cgtgctctcc ttgccggtct caaccaccgc gtacgcatgg ttggtgccga
     1921 gccggatcat catcgatctg ctgctcggct ggatgacctc gagcccgagc actgcctcgt
     1981 agaaccggcg tgccctggcg atgtcgacag tctcgagggt gccgtgcccg aggcgctgga
     2041 gcttgaggac aggttcaccg gtgaacttgt ctgtgattgc tggcattacg ggtccttttc
     2101 tcgttgtggg gttagagggc ggtcatacgc cggcctgtgg gctggcttgc atggcttgca
     2161 ccgcggcagc catctgggcc gagtccatgt actcgtcaat gcgccggatt cgaccgtccg
     2221 tcatggcgat ccgctggatg gcgggaatag aggccgactg tccgccgggc agagtgacgt
     2281 ggacgacgtg gcgttgaatg cacgcgccgt cgaccacgtc tcggccggcg atctcgtagc
     2341 gcggttgcag cgcgccgagg cctgcgagtg cagccagcgc ttcccttgcc ggctggccga
     2401 cctggtcgta gttgtgccag atcacggcgt catcggtgaa tagcgcatcg aggccggccg
     2461 catcgcccgt cgacaaagcc tcgacgaact tgtccacggt cgacagaaca tcgtggtggt
     2521 tgtcagtcac gtgactgccg ctccttgcct tggccggtca cggccatacc ttcggggtga
     2581 agatcgggcg ggcgatgcgc gaggcaatac gccatccgtc aacggttttc acgtaccggt
     2641 cgtggaattc gatgacgcgg ccgttgtcag tggcgtagac caccgcttcc ccgttctccg
     2701 ccaccggccc gtggtagacc atcgcgcaga ccaccgcctc ggccgcgtcg tcgtcgaggc
     2761 tggtgaggtg gaagttggtc agcaggtgcc gagtcgtcac cttcggccgt tccgccatgc
     2821 catcgcggat ctcgtcttgc ccacggtgga cgatgcctgc gcggtcgaac aatccgtcgg
     2881 gggtgaacaa ttcgatcatc gactcgaaaa cgccgttgtc gagatagaag gcgaaggcgt
     2941 tgttcagccg gacgagttcc cagtggaccg tcatcgcctg catggtcttc gtggctgccg
     3001 tcgtggccgt cgtcatggcg taccgtcagc ccagcgcagc cggcgggacg tcctcgtcga
     3061 cgagtagcgc gccgacgatc gtatagatct ccgcgctggc cgccgcatgc tgtgccgccg
     3121 tacggacgtc gcgcagacgt cgctgcaagg ttgaggtgtt gtacatggcg ttgcttcccg
     3181 ccaatccgaa agcctcgttg acgatctcga cgcattcggt gtgggcccgc gccaccatgg
     3241 cacgctggcg tgtcgcggcc agcggcgaga tgggctcgcg agagagcgcg gcatcccaca
     3301 tgagattcac ctccttctcg gccagcgctc gaaccgcggc aagtcggatc tcgagctggc
     3361 caagccggta ctggaacacg ggatccgcgg cgagccgctg ggtggggttg aacgcgggcc
     3421 gcttggtctt ggccagctcc cgcagatcgg cgagcgcacc ctcggcgata cccatcacca
     3481 ccgcgacgtg ggtgggcccc agcgccagcc ggaccggaag tcggaacagt ggcgtgtcga
     3541 cggtggccgg accgaagaac tgggcggtgt ggtcgttcga cacgaacacg tctttgagga
     3601 cgaagtcatt gctgttggtc ccgcacaatc cgactgagtc ccaggtctgc acgaactgtg
     3661 cgtcgcgcgc cggcaccatg gccagcacca tctccgggac gcccgcgtcg gtcatgatgg
     3721 gcgcaccgtt ccgcaacacc acgcagctgc cgatcatcca ctggtgctcg tagcttccgc
     3781 tggcgagggg ccactgtccg ttgacgagat agccaccatc ggtgcggatc gccaccccct
     3841 tgggtgcgaa tgccccgcgt gccatcgtgt ccggtccgtc gggataaatc tggccgtcga
     3901 ggacgtgctg cgggaatcgg ccgaataccg gggcgaagtc ggcaccgatc atcacggtcc
     3961 agcccgtgga cgcgtcggcc cgggcgacct cctcgatgac ctccatggcc gcggccaggc
     4021 tcatctcttc gccgccgtac tggcgaggga cgaacatgcg gaaagcccct gcggccgtga
     4081 ggcgttcgat cagatcggcg ggaaccacct tggctgcttc gatctcgccg gcgcgctcgg
     4141 cgatctcggg ggccagtgcc cggaccgcgt ccagcacggt ggtggtagcg gtgtcggcgt
     4201 cgatggcagt catgcatttc ctccagctga ccgcgcgtcc gatgcggcag ctgagccgtg
     4261 tctgaataaa tggatttgat tgaacacaag acgtattatg tgttcactac gctgccctgg
     4321 tcaagacctt cttcgaaact gttcgcggcc gcgctcgatc agcgcgaccg ttggccgtgg
     4381 ccgccaggcg ctatggccgg cgcgtcggcc tcgagtcgcc ggaaccggcc tgcgtggaac
     4441 accagcggag ccacggcggg gtgcgtacac aaccgatgaa tctgcaaaat ggcgagcgtg
     4501 tggtcgccgg cggggacctc gtcgaacagc gcgcactcca accacgcgga cgccccgccg
     4561 atcatcaccg cccctgtgcc ggttggcatc ca
//
LOCUS       DASYLX010000820         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3227124,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000820 DASYLX010000000
VERSION     DASYLX010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3227124"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGP27_19195"
     CDS             <1..732
                     /locus_tag="VGP27_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7853461.1"
                     /translation="VDYWFRMEMDGWENLPEPPVLLVGIHSGAPFVWDAWTVGVQWWR
                     RFGQDRLLHGTAHDALMAIPAIGRYFRAMGVLPAAPDSMATALAEGRDVAVWPGGEVD
                     SLRPWFDRDKANLAGRSGFVKMAIRAGVPIVPIATVGGADAMPVLIRGDRLSQTLRLD
                     KLLRLKVFPVAVSLPWGIAPAALPQFPLPAKIRTRLMPAIELDHDPQRVDDDQYVDAK
                     YHEVQSSIQSGMDALARKRALPLFG"
     gene            850..1692
                     /locus_tag="VGP27_19200"
     CDS             850..1692
                     /locus_tag="VGP27_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853462.1"
                     /translation="MALTQAIRVFSVVAALMFGLVFGPVVPTAGADTPAAQLKSATVK
                     KTTLTYTFALQSTEDVGGIWLTVRERDNPDNVIVNGQDVDVRPIPGAFRDITRTAGGM
                     PEGAAVCVSVVVWKYVHLGINDDPSPPSNQICTDPEKTASQADLALQNIRGKQDPLAP
                     ETYAYLILVDNNSANEAKDVTVDLATSGVATFAGDQGLVAPGWAAGGYSCAPRPPVGG
                     ESSAMTCRGGTLKAKDHTDPAVLVTFNSAGFGTIHAQVSGGGDTTPGNNGTALTLQPV
                     QKTG"
     gene            complement(1689..2528)
                     /locus_tag="VGP27_19205"
     CDS             complement(1689..2528)
                     /locus_tag="VGP27_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV7853463.1"
                     /translation="MRLATWNVNSIRARVDRVTDWLARADVDVLAMQETKCSDDQFPT
                     MPFAAIGYEVVHCGFNQWNGVAIASRVGIDDVQIGFDGQPTWSDSAAADVRSVRGPVA
                     EAAEARALGATCDGVQVWSLYIPNGRTVDSPHYGYKLAWLGALRDTAQQWLTDNPDAQ
                     IALTGDWNIAPTDEDVWSVEAYRGSTHVTGPERAAFNAIVGAGFSDVVRPFTPGPAVY
                     TYWDYTQLRFPKNRGMRIDFILGSPALAQRVSHAEIVREERKGKSPSDHAPVLVELGS
                     ARN"
     gene            complement(2538..3452)
                     /locus_tag="VGP27_19210"
     CDS             complement(2538..3452)
                     /locus_tag="VGP27_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7853464.1"
                     /translation="MSDLPAVGTRVSLRYRRPAGSVPPLTDVIGHLERTGPRIRVRTK
                     SGAVVEIAAGDVVAVRSLSAAPVRTSQIRATEHAAALAWPGTEQEWIDGWLLRGAGGH
                     THRANSAVPLGIGANVSSVPAIVDWYSGRGQTPWLAVADRLVDMPGHVAAHLETVVMV
                     RDLPAGQSDATVTLAPRPDARWLRLYERAVPVDVLSAVVDGVVVFATRDDAAVGRAAV
                     TTAPDGTRWAGMSAVRVADGRRREGHARALCLALMAWATEQGADRCYVQVLADNVPAI
                     GLYEQLGFTTQHRARYIDAAHVRSGVDP"
     gene            complement(3454..4047)
                     /locus_tag="VGP27_19215"
     CDS             complement(3454..4047)
                     /locus_tag="VGP27_19215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV7853465.1"
                     /translation="MAVVPIRIVGDPVLHTATTPVPVADDGSLPPDLADLITDLFETM
                     DAAYGVGLAANQIGVSKRVFVYDCAEERGKTARRRGVIVNPVLETSEVPETMPDPEND
                     DEGCLSVPGESFPTGRADWARVTGLDADGTPITLEGAGLFARMLQHETGHLDGLLYLD
                     RLVGRHARSAKRAVKSHGWGVPGLTWMPGEDPDPFGH"
     gene            4168..>4283
                     /locus_tag="VGP27_19220"
     CDS             4168..>4283
                     /locus_tag="VGP27_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEV7853466.1"
                     /translation="MDGAMARTEQSGDNSELTDGLTRREHDILAFERQWWKYA"
BASE COUNT          710 a         1462 c         1435 g          676 t
ORIGIN      
        1 gtcgactact ggttccgcat ggagatggac ggctgggaga atctcccgga accgccggtg
       61 ctgctcgtgg gcatccactc gggcgcaccg ttcgtctggg acgcatggac ggtcggcgtc
      121 cagtggtggc gtcgcttcgg ccaggaccgg ctcctgcacg gcaccgcgca cgacgcactg
      181 atggcgatac ctgcgatcgg caggtacttc cgcgcgatgg gcgtgcttcc cgccgccccg
      241 gactcgatgg cgaccgcact ggccgaaggg cgcgacgtcg ccgtgtggcc gggcggtgag
      301 gtcgactcgc tgcggccgtg gttcgatcgc gacaaagcca acttggcagg ccgcagcgga
      361 ttcgtgaaga tggccatccg cgcaggcgtt ccgatcgtgc cgatcgcgac cgtcggaggt
      421 gcagacgcga tgccggtgct gatccgcggc gaccggctct cgcagacgct gcgattggac
      481 aagctgttgc ggctgaaggt gttccccgtc gcggtatcgc tgccgtgggg gatagcgccc
      541 gcggcgctac ctcagttccc gttgcctgcg aagatcagga ctcgactcat gcccgcgatc
      601 gaactcgacc acgatccgca acgtgtcgac gatgaccagt acgtcgacgc gaaatatcac
      661 gaggtgcagt ccagcatcca gagcggaatg gatgcgttgg cgcgcaagcg ggccctgcca
      721 ctgttcggct gacggttgac ggttcgggcg cgtcgaaccg caaatcatag ggaaacacct
      781 gatttcttca cgggcccgca ctcaatagcg tccttggcag gaacctcaca gtcgaagggt
      841 gggtggctca tggcgcttac acaagcaatt cgcgtgtttt ccgtcgtcgc ggcgttgatg
      901 ttcggcctcg tgttcggccc tgtcgttccg acggccgggg ccgacacgcc cgcggcccag
      961 ctgaagagcg cgacggtcaa gaagacgacg ctgacctaca cgttcgcctt gcagagcacc
     1021 gaggatgtcg gcggcatctg gctgaccgtg cgggagcggg acaatccgga caacgtcatc
     1081 gtcaacgggc aggacgtcga cgtccgaccg atacccggag cgttccggga catcacccga
     1141 acggcaggcg gaatgccgga gggcgcagcg gtgtgtgtga gcgttgtcgt ctggaagtac
     1201 gtccacctcg ggatcaacga cgacccgtcg cccccgtcga atcagatctg cacggaccct
     1261 gagaagacgg ccagtcaggc cgacctcgcc ctgcagaaca tccgcggcaa gcaggatcct
     1321 ctcgcgcccg agacctacgc atacctgatc cttgtggaca acaacagcgc caacgaggcc
     1381 aaggatgtca ccgtcgatct cgccacctcg ggtgtcgcga cgttcgcggg ggaccagggg
     1441 ttggtcgcgc ccggctgggc ggcaggtgga tactcctgtg cgccaaggcc acccgtcggt
     1501 ggcgagtcct cggcgatgac gtgtagaggc ggcaccctga aggccaagga ccacaccgac
     1561 cccgccgtgt tggtgacgtt caacagtgcc ggcttcggga cgatccatgc acaggtgagc
     1621 gggggcgggg acaccacgcc gggcaacaac ggcaccgcgc tcacgctgca gcccgtccag
     1681 aagaccggtt agttccgcgc ggagccgagt tcgacaagca ccggggcatg atcgctgggg
     1741 gacttgcctt ttcgttcctc tcggacgatc tcggcgtgcg acacgcgttg cgccagcgct
     1801 ggggagccga ggatgaagtc gatgcgcatg ccccggttct tcgggaaccg cagctgggtg
     1861 tagtcccagt aggtgtagac cgcggggccc ggggtgaaag gtcgcaccac gtcgctgaac
     1921 cccgcgccga cgatcgcgtt gaacgcggcg cgctcgggcc cggtcacgtg tgtgctgccg
     1981 cggtacgcct ccacactcca cacgtcctcg tcggtgggtg cgatgttcca gtcgccggtc
     2041 aaagcgatct gcgcgtccgg gttgtcggtc agccactgct gcgcggtatc ccgcagcgcg
     2101 ccgagccagg ccagcttgta gccatagtgc ggggaatcca ccgtgcggcc gttcgggatg
     2161 tacaggctcc acacctgtac gccgtcacac gtcgcgccga gcgccctcgc ctcggccgct
     2221 tccgcgaccg gaccccttac gctcctcacg tccgcggcgg cgctgtcgct ccacgtcggc
     2281 tggccgtcga aaccgatctg cacgtcgtcg atgcccaccc gcgacgcgat cgccacgccg
     2341 ttccactggt tgaagccgca gtgcaccacc tcgtagccga tggcggcgaa cggcatggtg
     2401 gggaactggt cgtcggagca tttggtctcc tgcatggcca gcacgtcgac gtcggcgcgt
     2461 gccagccagt cggtgacgcg gtcgacgcgg gcccggatcg agttcacgtt ccaggtggcc
     2521 agccgcatgg ctacaggcta cggatctaca ccgctcctca cgtgcgcagc gtcgatgtag
     2581 cgcgcgcggt gctgcgtggt gaagccgagc tgctcgtaca aaccgatcgc gggcacgttg
     2641 tcggccagca cctgcacgta gcagcggtcc gcaccctgtt cggtggccca cgccatcagc
     2701 gcgaggcaca aggcgcgggc gtggccttct cgccgccgcc cgtcggcgac gcgcaccgcc
     2761 gacatgcccg cccagcgggt tccgtccggc gcggtggtca ccgccgcccg cccgaccgcg
     2821 gcgtcgtccc gagtcgcgaa caccacgacg ccgtcgacga cggcgctcaa cacgtcgacg
     2881 ggaaccgcgc gctcgtagag ccgcagccac cgcgcgtcag gacgcggcgc aagtgtcacg
     2941 gtcgcgtcgg actgacccgc aggcagatcg cggaccatga caaccgtttc aagatgcgcc
     3001 gcgacgtgtc ccggcatgtc gaccagccgg tcggccaccg ccagccatgg cgtctggccg
     3061 cgcccggagt accagtcgac gatcgcaggc accgagctta cattcgcgcc gatgccaagc
     3121 ggcacagcag aattcgctcg gtgagtgtgc cctcccgcac cgcgcagcag ccagccgtcg
     3181 atccactcct gctcggtgcc tggccacgcc agcgcggcgg catgctccgt cgcgcggatc
     3241 tgcgacgtcc gcaccggcgc cgccgacagc gaccgcaccg cgaccacgtc gccggccgcg
     3301 atctccacca ccgcaccgga tttcgtccga acacgaatgc ggggaccggt tcgttccaga
     3361 tgaccgatca cgtcggtgag cggtggcacc gaacccgcgg gccgccgata gcgaaggctg
     3421 acccgggtgc cgaccgcggg aaggtcggac acgtcagtgg ccgaacgggt cggggtcctc
     3481 acccggcatc cacgtcaggc ccggaacccc ccagccgtgc gacttcaccg cccgtttggc
     3541 gctgcgtgcg tgcctgccga ccaaccggtc caggtagagc aacccgtcca ggtgcccggt
     3601 ttcgtgttgc agcatccgcg cgaacaagcc ggcgccttcc aaggtgatcg gcgtgccgtc
     3661 ggcgtccagc cccgtcaccc gcgcccagtc ggcgcggccg gtcgggaacg actcgccggg
     3721 caccgacagg caaccttcgt cgtcgttctc gggatcgggc atcgtctcgg ggacctcgga
     3781 ggtctcgagc accgggttga cgatcacacc ccgcctgcgg gccgtcttgc cgcgctcctc
     3841 cgcgcagtcg tagacgaaca ctcgtttgga cacgccgatc tggttggccg ccaggccgac
     3901 cccgtacgcg gcgtccatcg tttcgaaaag atcggtgatc aagtcggcga gatcgggtgg
     3961 cagcgaaccg tcatcggcca ccggcaccgg agtggtcgcg gtgtgtaaga cgggatctcc
     4021 gacgattcgg atgggtacga ctgccacggt tggcaagctt aagtgagccg actcgcccgg
     4081 ttggctgacg gcccaacaag cgatgccgtg gttgaatatt cgccgtatcg ctagcgagtt
     4141 cgagtgcggg aaaggtccag gggcaacatg gacggcgcca tggcgcggac tgagcaatcc
     4201 ggggacaact ccgaactcac tgatgggctg acccgccgcg aacacgacat tctggcgttc
     4261 gaacgccagt ggtggaagta cgc
//
LOCUS       DASYLX010000821         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_484435,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000821 DASYLX010000000
VERSION     DASYLX010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484435"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VGP27_19225"
     CDS             complement(<1..207)
                     /locus_tag="VGP27_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853467.1"
                     /translation="MSEHESVWSDLQGVAFSQGYLDVAGVRTRYLHAGDTSRPAVVLL
                     HGSGGHAEAYVRNLEAHAEHFSTWS"
     gene            337..1041
                     /locus_tag="VGP27_19230"
     CDS             337..1041
                     /locus_tag="VGP27_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853468.1"
                     /translation="MPKTSAAGTGTNGAPGSQTLARGLAALQLVASAPNGLTAQQVAD
                     DIGVHRTIAYRLLTTLAQYRFVAKGEGGRYRSASALAVLGASFDNNVRQLSVPTLRSL
                     ADELGTTVSLLIAEGDQQVAIAVIVPTNVFYQLSFHEGSRYPLERGAAGIALLASMPP
                     RPGERELVPQTRKQGWVITHGEIEQNTYGLAVPVKRSPPSPPTCINLISHREDVVMGG
                     KPSVIKAANALSAILG"
     gene            1071..>1648
                     /locus_tag="VGP27_19235"
     CDS             1071..>1648
                     /locus_tag="VGP27_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853469.1"
                     /translation="MTGWDNEVDVVVLGSGGAGLTAALAAAANGASVEVYEKASTVGG
                     TTAVSGGIVWIPAHRRSPDGELTEADALRYLEAQSLGVMDRDLVETFVHTGPRMLDFV
                     EQHSELQFEIAEGFPDYKPELPGGRPTGGRSLNAKPFDLARLGEWKDRITSFPADFSN
                     VGIDAETRARIHAAVDSEAGDYCVAGTALIAG"
BASE COUNT          289 a          544 c          553 g          262 t
ORIGIN      
        1 cgaccaggtg gagaagtgct cggcatgcgc ctcgaggttg cggacatagg cctcggcgtg
       61 gccgcccgaa ccgtgcagca gcaccaccgc gggcctgctc gtgtcgcccg cgtgcagata
      121 ccgggtgcgc actcctgcga catcgaggta gccctgcgag aacgcgacgc cctggagatc
      181 gctccagacg ctttcgtgct ccgacacccc gtcacctctc ttgaggacga gaactgaatt
      241 ctcgtaattt gtaagcactc tgctcattta tcgcacatgc acgtgcacta tagtcagagc
      301 atcactctcg ggaatcggtc tgtcaaggag ggcgcgatgc cgaagaccag cgccgccggc
      361 accggaacca acggtgcgcc cggttcgcag acgctggcgc ggggtcttgc cgcgctgcag
      421 ctggtcgcca gcgcccccaa cggcctgacc gcccagcagg tcgcggacga catcggagtg
      481 caccgaacga tcgcctatcg cctgctcacc acgctggcgc agtatcggtt cgtggccaag
      541 ggcgagggcg gccggtaccg gtcggcgtca gcgctggcgg tgctgggggc atccttcgac
      601 aacaatgtcc ggcagctgag cgtgcccacg ttgcggtcgc tggccgacga actcggaacc
      661 acggtgtcgt tgctgattgc ggagggcgac cagcaggtcg ccatcgcggt gatcgtgccg
      721 acgaacgtgt tctatcagct gtcgttccac gagggcagcc gatacccgct ggagcgaggg
      781 gccgccggga tcgcactgct ggccagcatg cctccgcggc ctggcgagcg agagctcgtc
      841 ccgcagaccc gtaaacaggg ctgggtcatc acgcacggag agatcgagca gaacacctac
      901 ggtctggccg taccggtcaa acgcagtccg ccgtcgccgc cgacatgcat caacctgatc
      961 tctcatcgag aggacgtggt catgggtggc aaaccctcag tcatcaaggc ggccaacgca
     1021 ttatcggcga tcctcggatg agggaccgct ggcaggaagg gtgagcaacg gtgaccggtt
     1081 gggacaacga agtcgatgtc gtcgtgctcg gcagcggtgg cgcgggcctg acggccgccc
     1141 tggctgccgc agccaacggc gcatcggttg aggtgtacga gaaggcctcg accgtcggcg
     1201 gcaccaccgc ggtatccggc ggcatcgtct ggatacccgc gcatcgccgc tcgcccgacg
     1261 gcgagctgac ggaggccgac gcgctgcgct atctggaggc gcaatcgctc ggcgtgatgg
     1321 atcgcgacct cgtcgagacg ttcgtgcaca ccggcccgag gatgctcgac ttcgtcgagc
     1381 agcacagcga gctgcagttc gagatcgccg aggggttccc cgattacaag ccggagctgc
     1441 cgggtggacg gcccaccggt ggtcggtcgc tcaacgccaa gcccttcgac ctggcccgcc
     1501 tcggcgaatg gaaggaccgg atcacgtcgt ttcccgccga cttcagcaat gtcggcatcg
     1561 atgccgagac ccgggcccgc atccacgccg ccgtcgacag cgaagcaggc gactattgcg
     1621 tggcgggcac cgcgttgatc gccgggtt
//
LOCUS       DASYLX010000822         4155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2568828,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000822 DASYLX010000000
VERSION     DASYLX010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568828"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VGP27_19240"
     CDS             complement(<1..863)
                     /locus_tag="VGP27_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEV7853470.1"
                     /translation="MGVEEEFHLVDLKTRRLTARAPELLAELSDSYVAELQRCVVEMN
                     SEVVDTLDGLRADLQRQRKTLVDAATKLGIGVVAAGAVPLSVPAEMQVTQTPRYRQML
                     ADYQLLAREQLICGTQIHIGVDDRDKSVAVANRVAPFVPTLLALSASSPFWADGSDTG
                     YSSGRTLVWQRWPTTGLAAPVLSAAEFDQLVADLVSSGAIADKGMVYFDVRPAVEAPT
                     LELRVCDSCPSVDTIVLIAGLFRAMVEREMEGLRAGVPALAISAPLGRAALWRAARSG
                     LEGDLVDVTGP"
     gene            complement(1120..1192)
                     /locus_tag="VGP27_19245"
     tRNA            complement(1120..1192)
                     /locus_tag="VGP27_19245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Ala,seq:cgc)
     gene            complement(1256..2317)
                     /gene="glpR"
                     /locus_tag="VGP27_19250"
     CDS             complement(1256..2317)
                     /gene="glpR"
                     /locus_tag="VGP27_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HEV7853471.1"
                     /translation="MPSIPQSLLWISLVVLWLFVLVPMLISKRDAVRRTSDVALATRV
                     LNSTTSSRLLKRGGPASGHRSDPDWRPSEDDLDETYDDDVYDDDAYEDDDEEEEPQTA
                     VVLAAPVVEDAEPDYLDVDVIDEPVGALPVGATDTEQELGETGELSLNFEKPEETEEE
                     ENVDTAVYEIATDEYEEESDGTAEEYEYVEDSSGLEAPEDSDLRIADSMVAARRRRYD
                     PAKAAAISARKYKFRKRMLAFMTLVMMASAAAAYFVQPMGWYLCGGAGAVTLLYLGYL
                     RRQTRIEERLRRRRAKRAARARLGVENTEDEDLDLVPSRLRRPGSVVLEIDEEDPIFE
                     HLEYAPAARGYDLPRAAGQ"
     gene            2526..3401
                     /locus_tag="VGP27_19255"
     CDS             2526..3401
                     /locus_tag="VGP27_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853472.1"
                     /translation="MREELQWPFRGREAVAAGLLSDHRLRRSFAAVYPGVHVPRGAEL
                     SAAQRARAAWLWSRRRGVLAGLSAAATLGAKWIEPDLPAELVHTNRRPPPMLTVHTDD
                     LAAGETRRVGDMRVTTTARTAFDIGRRVDLVEGVQRVDALMNATDLKVSDIESVVALH
                     PGVRGLTQLRRTLDLVDSGAESPWESLTRLMFVRAGFPRPETQIHVYDGYGVLVAVID
                     MGWREYLVGVDFEGAHHWTNPRQRHWDVERFTRLPELGWQDFRVTSRMVRSAQRLVCN
                     RVGAALVARGCPKTW"
     gene            complement(3467..4120)
                     /locus_tag="VGP27_19260"
     CDS             complement(3467..4120)
                     /locus_tag="VGP27_19260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7853473.1"
                     /translation="MNLWHSSSAHPGWPMRVGPLRVPGGVVGLRPVRLRDGAVWSRIR
                     LADRRHLEPWEPSTDVNWVVRHSISQWPAVCSGLRSEARKGRMLPYVIEVDGEFGGQL
                     TIGNVTHGALRSAWIGYWVASSHTGGGVATAALALGMDHAFGPVRLHRLEATVRPENA
                     ASRAVLAKAGFREEGLLRRYLEVDGAWRDHLLVGITVEEVNGSVSSLLVREGHANWT"
BASE COUNT          693 a         1425 c         1318 g          719 t
ORIGIN      
        1 tgcggccccg tcacgtcaac gaggtcacct tcgagcccgg agcgggcggc gcgccacagc
       61 gcggcgcgac ccagcggtgc ggagatcgcc agggcgggca cccctgcgcg caatccctcc
      121 atctcacgtt cgaccattgc ccgaaacaga ccagcgatca gcacgatggt gtctaccgaa
      181 gggcagctgt cgcagacccg aagctcgagg gtgggcgcct cgaccgcagg ccggacgtcg
      241 aagtacacca tgcccttgtc ggcgatcgcg ccactgctga ccaggtcggc gaccagctgg
      301 tcgaattcgg cggccgacaa aactggtgct gccaggcccg tcgtcggcca tcgctgccac
      361 accaaggtcc gcccactgga atagccggtg tcggagccgt cggcccagaa gggtgagctt
      421 gcgctcaggg caaggagagt cggcacgaac ggcgcaaccc ggttggccac cgcaaccgac
      481 ttgtcgcggt cgtcgacgcc aatgtgtatc tgggtaccgc agatgagctg ttcgcgggcc
      541 agcagctggt agtccgccag catctgccgg tagcgcgggg tttgagtgac ctgcatctca
      601 gctggcaccg acaacggcac cgcccccgct gcgaccacgc cgatcccgag cttcgttgcg
      661 gcatcgacca gagtttttcg ctgtctttgc aggtcggccc gcagcccatc gagcgtgtcg
      721 acgacctcgc tgttcatctc gactacgcac cgctgaagct cggccacgta gctgtcggac
      781 aactcggcca agagctccgg cgctcgcgcg gtcaggcgtc tggtcttgag atcgacgaga
      841 tggaattcct cctcgactcc catcgtccgt agcgtcacgt ctggcaccta cccacgttaa
      901 ttgacggtca atacaaccgt gtaaagccgg acgccggtcc ttggaagtcc tacggactgg
      961 gcgtatcacc ctggcagcgc gatatgcgct tgaaccgtca atgcggtccg tgcggatgcg
     1021 aggagagggc aggaggccct tttggttcgc cagttgacgg gaaaagctga ggtgacgtgc
     1081 gcgcctacaa atagggattc tgaaatcggc caaaaactgt ggagctaagg ggaatcgaac
     1141 ccctgaccta ctcgatgcga acgagtcgcg ctaccaactg cgccatagcc cctggtcggt
     1201 agcacaggct accagtcaat ccgccgcggc agaaaccggc agacgtgacg ccggattact
     1261 gccccgccgc cctcggcagg tcgtagccgc gagccgccgg tgcgtactcc agatgctcga
     1321 agatcgggtc ctcttcgtcg atctccaaca ccaccgagcc tggcctgcgc aggcgcgacg
     1381 ggaccaggtc gaggtcctcg tcctcggtgt tctccacgcc gaggcgcgca cgtgccgctc
     1441 gtttggccct gcgtcgccgc agccgttcct cgatgcgcgt ctgcctgcgc agatatccca
     1501 ggtagagcag cgtcacggcg ccggcgccgc cgcagaggta ccaacccatc ggctgcacga
     1561 agtacgcggc tgccgccgac gccatcatca ccagcgtcat gaacgccagc atccgcttgc
     1621 ggaatttgta cttgcgggcg ctgatcgccg ctgccttcgc cgggtcgtag cggcgccgcc
     1681 gcgcggccac catcgagtcg gcgatccgca gatcgctgtc ttcaggggcc tcgaggcccg
     1741 aggagtcttc gacgtactca tactcctcgg cggtgccgtc cgactcttct tcgtattcgt
     1801 cggtagcgat ctcgtagacc gcggtgtcga cgttctcctc ttcttcggtt tcttcgggtt
     1861 tctcgaaatt tagtgacagc tcgccggttt cgccgagctc ctgctcggta tccgtcgcgc
     1921 cgaccggcag ggcacccacc ggttcgtcga tgacgtcaac gtcgaggtag tccggttcgg
     1981 catcctcgac gacaggcgcc gcgagcacca ccgccgtctg cggctcctct tcctcatcat
     2041 cgtcctcgta cgcatcgtcg tcgtacacgt cgtcgtcgta ggtctcgtcg aggtcgtcct
     2101 cggacggccg ccagtccgga tcactgcggt gacccgatgc gggtcccccg cgcttgagca
     2161 gccgggagct ggtcgtgctg ttgaggaccc gcgtcgccaa tgccacgtcg ctcgtgcgcc
     2221 gcacggcatc gcgcttgctg atcaacatcg gaaccagcac gaagagccag agcacaacga
     2281 gagagatcca caggagggat tgggggatgc ttggcatgac gccggctcct ttccccatca
     2341 ggctaggtcc gtgaccagcg catccatcgg tggcgcgccg agacaattac acacctgtaa
     2401 ttccctggtg acaagcacca acagtcacat atgtcacatt tgccacagca ctcttaaggc
     2461 gcgtctgtgc acagatcggc gcagtctggt ccgtgggtgt cggtcaccgt cggcaggctc
     2521 cggccatgcg ggaggaattg cagtggccgt ttcgcggccg cgaggccgtc gcggccggtc
     2581 tgttgtcgga ccatcgattg cggcggtcct tcgcggcggt ctatccgggc gtgcatgttc
     2641 cgcggggcgc tgagctttcc gcagctcaga gggctagagc agcgtggtta tggtcccgcc
     2701 gacgcggcgt gctggcgggt ttgtcagccg ccgcgactct gggcgcgaag tggatcgagc
     2761 ctgacctgcc cgccgagttg gtacacacga acaggcgccc cccgccgatg ctgacggtgc
     2821 acaccgacga cctcgcggcg ggcgagacgc gacgcgtcgg cgacatgcgc gtgacgacca
     2881 ccgcgcgcac cgcattcgac atcggcaggc gtgtcgatct cgtcgaaggc gtccagcgtg
     2941 tcgacgcact gatgaacgcg accgacctga aggtcagcga tatcgagtcg gtggtcgcgc
     3001 tgcatcccgg tgtgcgcggc ctaacgcagc tgcgtcggac gctcgatctt gtcgacagcg
     3061 gtgccgagtc gccatgggaa tcgctgaccc gactgatgtt cgtgcgcgct ggatttccgc
     3121 gaccggagac gcagatccac gtgtacgacg gatacggcgt gcttgtcgcg gtcatcgaca
     3181 tgggctggcg cgagtacctc gtcggcgtcg acttcgaggg tgcgcatcat tggaccaatc
     3241 cgcggcagcg gcattgggac gtcgagcgct tcacccgatt gcccgagctc ggctggcagg
     3301 actttcgggt gaccagtcgc atggtccgca gcgcgcagcg gctcgtctgc aaccgcgtcg
     3361 gcgcggcact cgtcgcccgc ggttgtccga agacgtggtg aatgtgcgga ttcggatgct
     3421 ttgcggtcga gatgtctccg aatccgcaca gtcgtgccgc cgaacgtcag gtccagttgg
     3481 cgtgtccttc gcggaccagc agcgagctca ccgagccgtt gacctcctcg accgtgatgc
     3541 ccaccaacaa gtgatcgcgc cacgcgccgt cgacctcgag atagcgcctc agcaggccct
     3601 cctcgcggaa cccggccttc gccaggacgg cgcggctcgc ggcgttctcc ggtcgcaccg
     3661 tcgcctccag ccgatgcagc ctgaccgggc cgaacgcatg gtccatgccg agcgccagcg
     3721 ccgcggtcgc caccccaccg cccgtatgcg agctcgccac ccagtagccg atccacgccg
     3781 accgcaacgc cccgtgggtg acgttgccga tcgtcagctg accaccgaac tccccgtcga
     3841 cctcaatcac gtagggcagc atgcgaccct tgcgggcctc cgaccgcagc cccgagcaga
     3901 ccgctggcca ttgcgaaatc gaatgccgca ccacccaatt gacgtccgtg ctcggctccc
     3961 atggttcgag atgcctgcgg tcggccagcc ggatgcggct ccacaccgcg ccgtcgcgca
     4021 gccgaaccgg gcgcagaccg accaccccgc ccggtacccg aagcgggccg acccgcatcg
     4081 gccagcctgg gtgagcggag gacgaatgcc acaggttcac gacgggattg tcgctcagcc
     4141 gcgctgcgcc aggaa
//
LOCUS       DASYLX010000823         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3227352,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000823 DASYLX010000000
VERSION     DASYLX010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3227352"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..707
                     /locus_tag="VGP27_19265"
     CDS             117..707
                     /locus_tag="VGP27_19265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV7853474.1"
                     /translation="MASQWRTFQRIAENLVFNGAPKVIRQLQRPENLPRNIQQGIKMG
                     LDALAGTTAAPPTAISAGRPVTNNSVPTAHRARKLVYAPDLDGRADPGEVVWTWVVYE
                     DDPSRGKDRPVLVVGRDRRTLLGLMLSSQERDQNERDWVGIGSGPWDYESRPSWVRLD
                     RVLDVPEEGIRREGAILERETFEIIAARLRRDYSWS"
     gene            complement(709..1314)
                     /locus_tag="VGP27_19270"
     CDS             complement(709..1314)
                     /locus_tag="VGP27_19270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7853475.1"
                     /translation="MAEIERRPIERADVRPLSKVLARAFYEDPLMLYMLPDDKARAKA
                     LPPMFAALTSHHFLSRGGSEVASRDRRIGAATLWDPPGQRKSSRWEELRMLPTLAWYF
                     RSRGKQTQALGKAMEDAHPEEPHWYLMVIGSDPAVRGAGFGQALMRSRLDRCDGERAP
                     AYLEASREELVGYYSRFGFEQIGEIQVPDGPKMWPMWRAPR"
     gene            complement(1307..1735)
                     /locus_tag="VGP27_19275"
     CDS             complement(1307..1735)
                     /locus_tag="VGP27_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV7853476.1"
                     /translation="MRIADVLRNKGAAVATITPETSVSGLLNELAVHNIGAMVVVSSD
                     GLVGIVSERDVVRKLHEHGAELLKLPVEAIMTTFVATCSPHDTVDHLTALMTTKRVRH
                     IPVLDNDRLVGIVSIGDIVKQRMEELESEHRALQDYITRG"
     gene            1871..2896
                     /locus_tag="VGP27_19280"
     CDS             1871..2896
                     /locus_tag="VGP27_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7853477.1"
                     /translation="MTNQSNDVPAVALGDELTVSAIGFGAMALTPVYGEVDDTESLAT
                     LHRCLDLGVTFIDTANVYGGGNNERLISKLLADRRDEVTLATKFGIASNPADRAAGTL
                     VARGDAEYVHKSIDESLSRLGTDVVDLYYMHRRDPSVPIEETVGAMAELVTAGKVRHL
                     GLSEVTADELRAAVAVHPIAAVQSEWSIWSRDVERNVVPAAAELGVGFVPYSPLGRGF
                     LTGTVRSAADLAASNDFRKTMPRFAEGALDANLVVVDVVTSVADALGATAAQVALAWL
                     RHRAETFGVASVPIPGTRRAARVEENLGSLWVTLTGDQLTALDAAADAVSGNRSRDLS
                     WVSAGRE"
     gene            complement(2901..3638)
                     /locus_tag="VGP27_19285"
     CDS             complement(2901..3638)
                     /locus_tag="VGP27_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853478.1"
                     /translation="MDANPAGTAATWLWQAGADIPKIVEFVAARQLREVYVAVPLSGV
                     DDNVAALTTALRANGIAVACLGGDPTWTVQHDTASNWAFRATTDAVFDAVHLDVEPWA
                     LPRWPQDARELMSSFATLVEEMTEVAPLVLDLVPWLVRDHREVLIRIARQCDSITVLA
                     YRDRASTIVAEAEPMLRLCETAGTRYRIGVETQPPSASVPTNTTFADDGEAVMRRELT
                     AVAARLQQPLFDGFAVHHLRSWQTMPP"
     gene            3708..>3828
                     /locus_tag="VGP27_19290"
     CDS             3708..>3828
                     /locus_tag="VGP27_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV7853479.1"
                     /translation="MIEVTLLGTGSPIPDPNRAGPSTLVRAGGQTFLVDCGRGV"
BASE COUNT          646 a         1255 c         1274 g          653 t
ORIGIN      
        1 ttatgggaat ctcctggtca ggctttgtat agcgggcgcg ctatgccctt ccagggtatc
       61 gagccggcgg cacggcgaca caatcgcgcg atttctcctt cacctatgct gcgaatatgg
      121 cgtcgcaatg gaggacgttt cagcggatcg cagagaacct ggtattcaac ggagcaccga
      181 aggtgatccg ccagctccaa cgacctgaga acctgcctcg caacatccag cagggcatca
      241 agatgggcct tgacgccctc gccggcacca ccgccgcccc cccgacggcg atcagcgccg
      301 ggcgcccggt gaccaacaac agcgtcccga cggcacatcg cgcccgcaaa cttgtgtacg
      361 cccctgacct cgacggccgc gcggatcccg gcgaagtggt gtggacgtgg gtggtctacg
      421 aggacgaccc gtcgcgagga aaggatcgcc cggtgctggt ggttggccgc gaccggcgca
      481 cgctgctggg cctgatgctg tccagccagg aacgcgacca gaatgagcgc gattgggtcg
      541 gcatcggttc cgggccatgg gactacgaat cccggccgag ctgggtgcgg ttggacagag
      601 tgctggacgt gcccgaggag ggcatccgac gcgaaggcgc gatcctggaa cgcgaaacgt
      661 tcgagatcat cgcggcgagg ctgcgccgcg actactcgtg gagctgacct accgcggcgc
      721 ccgccacatc ggccacatct tcgggccgtc gggaacctgg atctcgccga tctgctcgaa
      781 gccgaaccgg ctgtaataac cgaccagctc ttccctgctc gcttccaaat aggcgggcgc
      841 gcgttcaccg tcgcagcggt cgaggcgcga cctcatcagc gcctgcccga atccggcgcc
      901 gcgcacggcc gggtcgctac cgatgaccat cagataccag tgcggttcct cggggtgagc
      961 gtcctccata gccttgccga gggcctgcgt ctgcttgcca cgcgaacgga agtaccacgc
     1021 cagcgtcggc aacatccgga gctcttccca tcgcgacgac ttgcgttgcc ccggcggatc
     1081 ccacagcgtc gccgcgccga tccgccggtc gcggctcgcc acttccgacc caccgcggga
     1141 caggaaatga tggctggtca gcgccgcgaa catcggcggc agcgccttcg cccttgcctt
     1201 gtcgtcgggc agcatataca gcatcaacgg gtcctcgtag aacgcccggg ccagcacttt
     1261 cgacaaggga cgaacgtcgg cacgctcgat gggccgacgt tcgatctcag ccacgggtga
     1321 tgtagtcctg cagggccctg tgctcgctct ccagttcctc catgcgctgt ttcacgatgt
     1381 cgccgatgct gacgatgccg accagccggt cgttgtccag cacagggatg tgtcggaccc
     1441 gcttcgtggt catcagcgcg gtgagatggt cgaccgtgtc gtgcggcgag caggtcgcga
     1501 cgaacgtcgt catgatggcc tcgacgggca gtttcagcag ttcagcgccg tgctcatgca
     1561 gctttcgcac gacgtcgcgc tcggagacga tgccgacgag cccgtcggac gacaccacca
     1621 ccatggcgcc gatgttgtgc acagcgagct cgttgagcag cccggacacc gaggtctcgg
     1681 gggtgatcgt cgccaccgcg gcgcccttgt ttcgcaacac gtccgcgatt cgcatcgtcg
     1741 cctccatcgt gacctggttc acacaaggtt aggtcgaagt tggcccaggg gaaagcatct
     1801 tggtcggaac agtcacgcgc cggcgctggt tgcagcacca tgaaccaccg tcgacttgag
     1861 gaatgaaagc atgactaacc aatctaatga cgtgcccgca gtcgctctcg gcgacgaatt
     1921 gacggtcagt gcaatcggtt tcggagcgat ggcgttgacg cccgtatacg gagaggtcga
     1981 tgacaccgaa tcgctggcca ccctgcaccg ctgcctcgac ctcggcgtca cgttcatcga
     2041 caccgccaac gtgtacggcg gcggaaacaa cgaacggctg atctccaagc tgcttgccga
     2101 ccgacgtgac gaagtcaccc tggcaacgaa gttcggcatt gcgagcaacc cggccgatcg
     2161 agcggcaggg actctggtgg cgcgcgggga cgccgagtat gtccacaagt ccatcgacga
     2221 gagcctgtcg cggctgggca ccgacgtcgt cgacctctat tacatgcatc gccgcgatcc
     2281 gtcggtgccg atcgaggaaa ctgtgggcgc gatggccgag ctggtaaccg cgggcaaggt
     2341 gcgccacctc ggcctgtcgg aggtcaccgc cgacgaactg cgggccgcgg tcgcggtgca
     2401 cccgatcgcc gcggtgcaga gcgagtggtc gatctggagc cgtgacgtcg agcgcaatgt
     2461 cgtgcccgcg gccgccgagc tgggcgtggg cttcgttccg tactcgccgt tggggcgtgg
     2521 cttcctgacc ggcaccgtgc ggtcggcagc cgatctcgcg gcgagcaacg acttccgcaa
     2581 gacgatgccc cgcttcgccg agggtgcgct cgatgcgaac ctcgtggtgg tcgacgtcgt
     2641 cacgtcggtg gccgatgcgt tgggcgcgac ggccgcccag gtggcgctgg cctggttgcg
     2701 gcaccgcgcc gaaaccttcg gtgtggcgtc ggtgcctatt ccgggaaccc ggagggccgc
     2761 ccgggtcgag gagaatctcg gctcgttgtg ggtgacgctg accggggatc agctcaccgc
     2821 attggatgcc gccgcagatg ccgtgtccgg gaacaggtcc cgcgatttga gctgggtgtc
     2881 ggccggccgc gagtagccgg tcacggcggc atggtctgcc acgagcgaag gtggtggacg
     2941 gcgaacccgt cgaacaacgg ctgctgaagc cgtgcggcga cggcggtgag ctccctgcgc
     3001 atcaccgcct cgccgtcgtc ggcgaacgtc gtgttggtcg gtaccgaggc tgacggcggc
     3061 tgggtctcga cgccgattcg gtagcgagtt cccgcggttt cacaaagccg gagcatcggt
     3121 tcggcctccg cgacgatggt cgaggcacgg tctcggtagg ccagcaccgt gatcgaatca
     3181 cactgacgtg caatgcgaat gagtacttca cggtgatcgc gcacaagcca cggcaccaga
     3241 tccagcacca gcggagccac ctccgtcatc tcctccacca gagtggcgaa ggaactcatc
     3301 agttcgcgtg catcctgcgg ccaacggggc agtgcccacg gttcgacgtc gaggtggacg
     3361 gcgtcgaaca cggcgtcggt ggtggcccgg aacgcccaat tggacgcggt gtcatgctgg
     3421 accgtccacg tcgggtcgcc gccgaggcac gccaccgcaa ttccgttggc ccgcaacgct
     3481 gtggtgagcg ccgcgacgtt gtcgtcgacc ccgctgagtg gcaccgcgac gtagacctcg
     3541 cgaagctggc gcgcggccac gaactcgacg atcttcggga tgtctgctcc tgcttgccac
     3601 agccacgttg cggccgttcc cgccggattc gcgtccacag atctcacgtt aggcaatttc
     3661 tggcgatatg gcctgagctg ggccgttcga cggggtaggt atgtctgatg atcgaagtca
     3721 ccctgctcgg caccggcagt ccgattcccg accccaaccg ggcgggcccg tcgacgctgg
     3781 tgcgggccgg tgggcagacg ttcctggtcg actgcggtcg tggcgtgc
//
LOCUS       DASYLX010000824         6672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3666230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000824 DASYLX010000000
VERSION     DASYLX010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGP27_19295"
     CDS             complement(<1..367)
                     /locus_tag="VGP27_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV7853480.1"
                     /translation="MSSDIVTIGVYGFDLPDFLGALRSANVGVLADVRQRRGVRGSRY
                     TWANARRLQATLAEEGIAYRHHRELAPTTELRHLQYAADDRLGVGKRSRELLDPEYLH
                     RYITEILDRADLDALVAEIP"
     gene            393..746
                     /locus_tag="VGP27_19300"
     CDS             393..746
                     /locus_tag="VGP27_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEV7853481.1"
                     /translation="MLIDDRIDAIGRQPFRAKFRLRGRERAIVQLRGMGAVRRHAEEL
                     FAQRVAPAQPRKDGRQTPYRGHPVFVAQHATATCCRTCLERWHEIPKGHALDAREQAY
                     AVDVICRWIERELEP"
     gene            817..889
                     /locus_tag="VGP27_19305"
     tRNA            817..889
                     /locus_tag="VGP27_19305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:850..852,aa:Thr,seq:cgt)
     gene            complement(892..1602)
                     /locus_tag="VGP27_19310"
     CDS             complement(892..1602)
                     /locus_tag="VGP27_19310"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7853482.1"
                     /translation="MTDNPVSRVAKPRQPGGREVPPVPSATVEQLRARLGPVDALMVS
                     VLAYAGLRPQELLGLQWNDIHERAILVRPKNVDGQLYPYTKTRQNRRVKLLAPLATDL
                     AEWKLASGRRDGLVVPRTDGKPWRDHDYRNRRRRIYKPHAAAVGLTSGVPYDLRGSFV
                     SLLAWEGQTMLEVARQAGHSVAICERHYAGIFEDYDPAQRTSAEAAIRAAREPGVRGV
                     CALVDAGTEAEMQNAWKP"
     gene            complement(1852..2565)
                     /locus_tag="VGP27_19315"
     CDS             complement(1852..2565)
                     /locus_tag="VGP27_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEV7853483.1"
                     /translation="MSHAHQHRIRNSLLAGTMVMAIGLLGGTAAAQASPCVVGPGVTQ
                     TDTVVTGSPGNDTIDCGGTTPGKTINGNGGNDTITGTDFNDTINGGDGNDTLTGGTGD
                     DSLNGGLGIDTISGSAGNDTLVGASNDGSQDSLDGGLGTDTCQGPAPDPDIHANCENT
                     TTPPTSGPGSGTANATELCQATGGVFNLTVNPAGYACVFNILNPADRRVDEASKICTG
                     RSGTFVNLLPASYACVLPH"
     gene            2791..3291
                     /locus_tag="VGP27_19320"
     CDS             2791..3291
                     /locus_tag="VGP27_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853484.1"
                     /translation="MKEREHLRGNAVDSAVDDADEGDREATRNLRNGKHHFFFLESGY
                     LDTDDMLAAAGVGRRAGIVLWCCVTCVSGRQACGLVGDPSLCPRKLAKRSTANTAVAQ
                     SSRHTSRVGGPVSSWLHELVGSAREAAEERSQLGGDTKNGAFDDADDGGDETHENTSL
                     SAGAQL"
     gene            3444..3764
                     /locus_tag="VGP27_19325"
     CDS             3444..3764
                     /locus_tag="VGP27_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853485.1"
                     /translation="MAKWSFLTNHARVLVCIAHDPGARLREMAAALDITERSAFGIVT
                     ELVEAGYVVKDKEGRRNRYRIQGHLPLRDSVARERTIGEVLDVLVKASPLPTQTSADT
                     SPVP"
     gene            4353..4883
                     /locus_tag="VGP27_19330"
     CDS             4353..4883
                     /locus_tag="VGP27_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853486.1"
                     /translation="MTGQHNGAINVAGGQTQCLDNATQTGEITVDPNGTLSVRKSTIT
                     GAVTLFSGFSEFEFCGSKTVGGAISATGGKGPVTIGSIGDTGQPACDPNTVGGAVTLD
                     ANQTGVTLARNQVDEAVTASGNLGGTKIAGNQIVGALTCTDNIAAPDNIGLLAQPMPN
                     TVGGARFGQTCLNGGF"
     gene            5209..6387
                     /locus_tag="VGP27_19335"
     CDS             5209..6387
                     /locus_tag="VGP27_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853487.1"
                     /translation="MARRRERLVPTHHRALTPAPTASKREIGLLRLQRLAGNRAVRHL
                     LREAAPLLGGLDAGTKQKLQIATVNVPSDFADDETFARADPKELKDFDVQFGAKVPKD
                     QNLRKGVKVVAWDMFDPAGQPNDVESPFRNNSTVTLELDLKAFKGENGLWQFTIVTTG
                     KSPKKHLLIDYIGPAPKYDASDAAAKRFGELGMKLRSGTAGFRPDEKDAIYSAVSLLP
                     AVAVAKLPKNLSFVRDHTPHPGGKCTTATAKAGGAYCSADHTIQLFDRWKDSQVRYAR
                     ATSQTATVLHELGHAIDDANKDAHAAFAKAVASDGGTAISGYGGTSTFESFAECFFLF
                     IADPKMLEALRPNVYAYFVGTYGAAGSTTPPSGSGAAAGKTSATPVKAGSGSAAAPKH
                     "
BASE COUNT         1106 a         2165 c         2289 g         1112 t
ORIGIN      
        1 tcggaatctc ggcgacgagc gcgtcgaggt cggcgcggtc gaggatctcg gtgatgtagc
       61 ggtggaggta ctcgggatcg agcagctccc gtgagcgctt gccgacgcca agccggtcgt
      121 cggcggcgta ctggaggtgg cgcagctcgg tcgtcggcgc gagctcacgg tgatggcggt
      181 aggcgattcc ctcctcggcg agcgttgcct gcaggcgccg ggcgttcgcc caggtgtagc
      241 ggctcccgcg cacgccgcgg cgctggcgca cgtcggccag caccccgacg ttcgccgacc
      301 gcagcgcgcc gaggaagtcc ggcagatcga agccgtagac gccgatcgtg acgatgtcag
      361 aggacacgag ctacgactct aggctggacg cgatgctcat cgacgatcgc atcgacgcga
      421 taggccgcca accgttccgg gccaagttcc gcctgcgcgg gcgcgagcgc gcgatcgtcc
      481 agctccgcgg catgggagcc gtccgcaggc acgccgagga gctcttcgcg cagcgcgtcg
      541 ccccagcgca gccgcgcaag gacggccgcc agacgccgta ccgcggccac ccggtgttcg
      601 tcgcccagca cgcgaccgcg acgtgctgcc gcacatgcct cgagcgctgg cacgagatcc
      661 ccaagggcca cgccctcgac gcgcgcgagc aggcctacgc cgtcgacgtc atctgccgct
      721 ggatcgagcg cgagctggag ccatgagcac gcgcgggcgg cgggcgcccg ccgctggccg
      781 acctgaccgc accggcgcta gcattggcgg ccgcccgccg acgtagctca gctggtagag
      841 cacttcactc gtaatgaagg ggtccccggt tcgagtccgg gcgtcggctt ctcacggttt
      901 ccaggcgttt tgcatctcag cctcggtgcc cgcatcgact agcgcgcaca ctccgcgcac
      961 accaggttcg cgcgcggccc ggatagcggc ctcggcgctc gtgcgctggg ccgggtcgta
     1021 gtcctcgaag atcccggcgt agtggcgctc gcagatggcc accgagtggc ccgcctggcg
     1081 agcgacctca agcatcgtct gaccctccca ggccaacagc gacacaaacg agccgcgcaa
     1141 gtcgtagggc acgccggacg tcagcccgac ggctgcggcg tgcggcttgt agatccgtcg
     1201 gcgccggttg cggtagtcgt gatcgcgcca cggcttgccg tcggttcgcg gcacgacgag
     1261 cccgtcgcgg cggccgctgg cgagcttcca ttcggccagg tcggttgcga gtggtgcgag
     1321 cagcttgacg cgtcggttct ggcgggtctt ggtgtagggg tagagctggc cgtcgacgtt
     1381 cttgggacgc acgaggatcg cccgctcgtg gatgtcgttc cattgcagcc cgagcagctc
     1441 ttgtgggcgc agcccggcgt aggcgagcac ggacaccatc agcgcgtcga cgggcccgag
     1501 gcgtgcacgt agctgctcga ccgtggccga gggaacggga ggcacctctc gaccaccggg
     1561 ctgcctgggc ttggcgacgc ggctgacggg attgtcggtc accctgctcg cttgcaacgc
     1621 cgccactcgc gtgtatctgt cagtggagtg cctctctcgg cgagacgggg gcgcgctgcg
     1681 gagggtgtcg agttcgtggc tgcggtgttc ttgacgcggg acagtgtggc tgggctacga
     1741 actctggccc gatgagcacc gcgagtgtgc tgatggttac gagccggagg gccgctgtct
     1801 caagcggccc tccggtccaa ctgcgtgccg gctgtgtttg gtgaagctag atcagtgtgg
     1861 taggacgcag gcgtagctcg ccgggagcag gttgacgaac gttccgctgc gcccggtgca
     1921 gatcttgctg gcttcatcga cgcggcggtc agcggggttg aggatgttga acacgcaggc
     1981 gtatcccgcg gggttcacgg tgaggttgaa aactccgccg gtggcttggc acagttcggt
     2041 tgcgtttgcc gtgcccgagc ccggtccgct tgttggcggc gtggtcgtgt tctcgcagtt
     2101 ggcgtggatg tcggggtcgg gtgcgggtcc ttggcatgtg tcggtgccca gtccgccgtc
     2161 gaggctgtcc tggctgccgt cgttggaggc gccaacgagg gtgtcgttgc cggcgctgcc
     2221 ggagattgtg tcgatgccga ggccgccgtt gaggctgtcg tcgccggtgc cgccggtgag
     2281 ggtgtcgttg ccgtcgccgc cgttgattgt gtcgttgaag tctgtgccgg tgatcgtgtc
     2341 gtttccgccg ttgccgttga tcgtcttgcc gggtgtcgtc ccgccgcagt cgatggtgtc
     2401 gttgccggga cttccggtca cgaccgtatc ggtctgcgtc acgccggggc ctacgacgca
     2461 gggggaggct tgggctgcgg ctgtgccgcc gagcaacccg atggccatga ccatggtgcc
     2521 ggcgagcagg ctgttgcgga ttcgatgctg atgagcgtgg ctcatgaggt tctcctcttt
     2581 ggcacgtcga gttgacggac agttccgtgg cgcgcgtggc accaagaacg tcctgtgtga
     2641 ttggacatcg agactcggca gagcgccggg tacgccgtcg ggggtagcga cgaaacgcgt
     2701 cgcgctccgc cggcgggcag cggcgagcgt gttgtcagct cagccgccgg tgaactgttg
     2761 tgttcagacg gggggtgcga gcccgagcat gtgaaggagc gcgaacacct gcggggcaat
     2821 gccgttgata gcgccgttga tgatgccgac gagggagacc gcgaggccac caggaatctg
     2881 aggaacggga agcatcactt cttctttcta gagagcggct atttggatac ggatgacatg
     2941 ctcgcggcgg ccggtgtggg tcggcgtgcc gggattgtcc tgtggtgctg tgtgacgtgt
     3001 gtctcgggcc gccaggcgtg cggcctggtg ggcgatccgt cgctgtgccc gcgcaaactc
     3061 gcgaagcgct ctacggccaa cacggccgtc gcgcagtcca gtcgtcatac gagccgcgtg
     3121 ggtggccctg tgtcatcttg gctgcatgaa ctagtgggtt ccgcccggga ggccgccgag
     3181 gaacgcagcc agctgggggg cgataccaag aatggcgcct ttgacgatgc cgatgacgga
     3241 ggcgatgaga cccatgagaa cacttcgctt tctgcgggcg ctcagttgta gaaagttctg
     3301 ctcgtggccg ctacagcaac ctacagcaga aaccgcaagc tagcaaccaa ttgctagaat
     3361 ccgatacaag gtctaaaata acgcgaaata aactactact ttagcgagct acttctggaa
     3421 tgcctgtgct atagtcggcg tgtatggcga agtggagctt cttgaccaac cacgctcgcg
     3481 tgctggtttg catcgcgcac gaccctggcg cgcgtctgcg cgagatggct gctgcgctgg
     3541 atatcaccga gcgcagcgcc ttcgggatcg tcacggagct ggtcgaagcc ggctacgtcg
     3601 tcaaggacaa ggaaggtcgt cgcaaccgct atcgcatcca gggccatctg ccgttgcgcg
     3661 actccgtcgc tcgcgagcga actatcggcg aggtattaga cgtgctcgtc aaagccagcc
     3721 ccctgccaac gcagaccagc gcggacacga gcccggtgcc ttgacgcgtg gccgtcccgc
     3781 atgatcggtt gacctcgtac gccgccggta gcgtcgaatg tccaacgcaa cgacctgctg
     3841 cgtagagatc gaaggcaagt agcgcgcggc gccggctagt ccagtgagtt gccgtacgag
     3901 ctgcatgtgt gtcgacgttc agcgggcagc aggcagggcg agcttgccgc cgccgcgcga
     3961 cttcaggcaa atcgccaact ggcactcgcg tctcccggcc gccctggcgg ctcgggacgc
     4021 atccgaaacc cgcgggagcc aagcgtatgc accccacttg ccgatccctc ggcaagccgg
     4081 ggcgaagcca cgacagcatt tacgagccgt gcgatactag aaattagttg ctagtgccga
     4141 cctgctgata taggttgcct gggcggggta gccacgaacc tcctacaact cggcgccccc
     4201 aagaggagca tctcttcatg aaccgcctgt accgccgaca tatccgtcgg gggactgtcg
     4261 ccacgctcac cggagccctt acgctcttga ccgttggaac aattgctccg ctggccgctc
     4321 aggccgcgat cacgtgcacc acggcgaaca caatgaccgg tcaacacaac ggcgccatca
     4381 atgttgccgg cggccagaca cagtgcctcg ataatgccac tcagaccggc gaaataaccg
     4441 tggatcccaa cggaacgctt tccgtgcgca agtccaccat taccggggcc gtaaccctgt
     4501 tctcgggctt ctccgagttc gaattttgcg gcagcaagac cgttggaggc gccatctcgg
     4561 ccactggcgg caagggaccc gtcaccatcg gtagcatcgg ggacaccggt cagccggcct
     4621 gcgaccccaa caccgtcggt ggtgcagtca ccctcgacgc caaccagacc ggcgtgacgc
     4681 tcgcgcgaaa ccaggtcgac gaggcagtga ccgcgagcgg gaacctcggt gggacgaaga
     4741 tcgccggaaa tcaaatcgtc ggcgccctga cctgcaccga caacattgcg gcccctgaca
     4801 acatcggctt gctagcccag ccgatgccca acaccgtagg tggggcgcgg ttcggccaga
     4861 cgtgcctgaa cggcggcttc taaggaccat cagcggcctc gacccgcaca gcgcgggttg
     4921 aggccacact gctcccagcg cccgacgaag cctcggtgac tgccggagtg gtcgacctcg
     4981 cgcgcgcaac ccgggaggtg cataggctga gagggcctcg caggacggct accgcaaaga
     5041 ccgcagcgca acgccgacat cgacagcgcc caatcgtcat gggaaggggt ccccggttcg
     5101 agtccgggcg tcggcttccg gcttcctgca ctgcacgttc gaccgtccac tcgggcacaa
     5161 ctcaccggcg tccttgactg gccccacacc gcctgatggg ctcttcggat ggcgcgacgt
     5221 cgcgaacgac tggtcccgac ccaccatcga gcgctgactc cggcgccgac cgcgtccaag
     5281 cgcgagatcg ggttgctgcg actccagcgg ctcgccggca accgcgcggt tcgccacctg
     5341 ctgcgcgagg cggcgcccct gctcggtggc ctcgatgcgg ggacgaagca gaagctccag
     5401 atcgcaacgg tgaacgtccc gtcggacttc gccgacgacg agaccttcgc gcgcgcggac
     5461 ccgaaggagc tcaaggactt cgatgtgcag ttcggcgcca aggtgcccaa ggaccagaac
     5521 ctgcgcaagg gcgtcaaggt cgtcgcctgg gacatgttcg acccggcggg ccagccgaac
     5581 gacgtcgaga gcccgttccg caacaactcc actgtcacgc tcgagctcga cctgaaggcg
     5641 ttcaagggcg agaacggcct gtggcagttc accatcgtga cgacgggcaa gtcaccgaag
     5701 aagcacctgc tgatcgatta catcggtccc gctccgaagt acgacgcttc agacgcggcg
     5761 gcaaagcggt tcggagagct cgggatgaag ctgcggtcgg ggactgctgg cttccgtccc
     5821 gatgagaagg acgcgatcta cagcgcggtc agcctgctgc cggcggtcgc ggtggcgaag
     5881 ctgcccaaga acctctcgtt cgttcgcgac cacacaccgc acccaggggg aaagtgcacg
     5941 actgcaacgg ccaaggcggg cggtgcgtat tgctcggccg accacacgat ccaactgttc
     6001 gaccgctgga aggactcaca ggtgcggtac gcgcgcgcca cgagccagac cgcgacggtg
     6061 ttgcacgaac tcgggcacgc gatcgacgac gcaaacaagg acgctcacgc tgcgttcgcg
     6121 aaggccgtcg ccagcgacgg agggaccgcg atcagcggat acggtgggac gagcacgttc
     6181 gagagcttcg cggagtgctt cttcctgttc atcgccgatc ccaagatgct cgaggcactg
     6241 cgtcccaacg tgtatgccta cttcgtgggc acgtacggcg cggcggggtc aaccaccccg
     6301 ccgagcggga gcggggcggc cgccggcaag accagcgcca cacccgtcaa ggccggcagc
     6361 ggcagcgccg ctgcgccgaa gcactgaccg ctcccggggc aacggccacg gccgcgaccc
     6421 gagaatcgtg cccgatcgtt caccagtcgg ttcccgacgc gagccggtgc cggccgtgcg
     6481 cgccgcgcag gagtgctcga cgcccgcagc gccgatgccc actaggctgc ccgccgctgc
     6541 gccggtgctg gccccgccgc ggacgcgacg ggtccgccat tgtcgttgag cttgatctgc
     6601 cggatgcgtg acgtccgttc gcgggtggcg ttcgctggtc cgaagatgtc agcgacccgc
     6661 agcgtctgct cg
//
LOCUS       DASYLX010000825        12461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1362287,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000825 DASYLX010000000
VERSION     DASYLX010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362287"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..385)
                     /locus_tag="VGP27_19340"
     CDS             complement(143..385)
                     /locus_tag="VGP27_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853488.1"
                     /translation="MKLTNYTNNFRRYGYTDADFEGGGSDRLIDGLVLHGSADTVAAG
                     VHSHLDAGADHIGIQVLTPDGENPMPGYRELARVLL"
     gene            634..1209
                     /locus_tag="VGP27_19345"
     CDS             634..1209
                     /locus_tag="VGP27_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853489.1"
                     /translation="MRRSRAALFTAVAELVSTAGSTDLSLAAIAEAADVSRQVIYQQF
                     GDRDTLVVQTALGLLRHDVLPEIADRPTLAPRERALVTAKHFAAHHRFYRPVLAGSCA
                     AAFSRGLTELHLPPNRVLVDQILGEHRDPQTAEDLAVYLTGGGIAFLTAWVLNGSEHQ
                     DCERFVDRLGYLHAALIRTEGPLPRPEGTDS"
     gene            1206..2033
                     /locus_tag="VGP27_19350"
     CDS             1206..2033
                     /locus_tag="VGP27_19350"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019684141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7853490.1"
                     /translation="MSLDLAGRVAVVTGGASGLGKSIVERFVAEGMRVVIADVNDIDG
                     ERLANLHGSDVLFRPTDVAVPEQVTALVETAVSQFGGLDVMVNNAGVAGTRHWDFLDD
                     DLADFDRVMSVNLLGVMVGTRDAARHMADHGGGSIINMSSIGGIEASPGVMPYGASKA
                     AVIHFTKAAALALAQYEIRVNCIAPGHIRTPLLATSAPDLSPDDLAQYEAEIRAIMGS
                     QRPLQREGKAADIAGAAAYFAGDDSVYVTGTVLPVDGGVVAGNTAKPPRFTPKGDKQ"
     gene            2030..2224
                     /locus_tag="VGP27_19355"
     CDS             2030..2224
                     /locus_tag="VGP27_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7853491.1"
                     /translation="MKVTVESRKCIAAGMCVASAPEVFDQDDDNGIVVLLDERPGADQ
                     IDNVRDAADVCPAQVISLQD"
     gene            2252..3481
                     /locus_tag="VGP27_19360"
     CDS             2252..3481
                     /locus_tag="VGP27_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853492.1"
                     /translation="MSATSAHPTAHEQLVDYPMERSSACPFAPPEQLREVAARAPICR
                     VRIWDGSTPWLVSGYAQMKRLTSDPRVSVDDTLAGFPHWNAFVAQYHRPRSVFNSDGE
                     QHSHYRRMMTKPFTVKRVNALRPAIQQIVDTKIDELLAGPNPADLVTALAVPVPSLVI
                     SEILGVPEQDRAYFEQEAFHATDHEATVEEKMASARSLMDYLKNLVAAKMASPADDHV
                     SELSQRVEAGEIPLEEAAYLATGLLSAGHETTANMIGLGTAALLQHPEQLALMRDSIE
                     PEAIASAVNELLRYLSIIHNGQRRIALADIDIDGVHIAAGEGIILDLSPANWDETVFP
                     DAGRLDLRRPAGNHVAFGFGPHQCVGQQLARAELQIVYPALLRRVPSLRLAIPAEDIA
                     FMYRELAFGVYSLPVTW"
     gene            3508..4416
                     /locus_tag="VGP27_19365"
     CDS             3508..4416
                     /locus_tag="VGP27_19365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853493.1"
                     /translation="MTRTEHDTWDTTSSVGATATGCAVVRALASRQSHPLIDDPFAAA
                     LVQQAGVDAFTQVVDGQLAPSDVFGPLAPAIIDSMAVRTASHDYFALDRVAQGTAQVV
                     MLASGLDTRAYRLAWTSGTTVFDVDLESVTTFKSTTLAALGFSPSSDHRPVGADLRYD
                     WSTRLLAAGFDPGKRAVWLVEGLLPYLPPDAQDALLDNITGLSAPDSALIIESVTDAR
                     SPEHDEFQAELDQVSERQRQQGHDLDIGTLMYEGDRHETASYLNTAGWTTTQVSATEL
                     FASCGRPLAHGERPFPDGRTVLATKG"
     gene            5561..5785
                     /locus_tag="VGP27_19370"
     CDS             5561..5785
                     /locus_tag="VGP27_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853494.1"
                     /translation="MGIGRKYIAPMLGAMAAVAAIGTAPLAAGEEPSDAQQPPPPGQS
                     CVQLGTSQFKCESPGDVQLNDAPSVTNNPY"
     gene            complement(6144..6350)
                     /gene="thiG"
                     /locus_tag="VGP27_19375"
                     /pseudo
     CDS             complement(6144..6350)
                     /gene="thiG"
                     /locus_tag="VGP27_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740712.1"
                     /note="functions in thiamine (vitamin B1) biosynthesis; in
                     Bacillus subtilis this enzyme catalyzes the formation of
                     thiazole from dehydroxyglycine and
                     1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
     gene            complement(6990..8327)
                     /locus_tag="VGP27_19380"
     CDS             complement(6990..8327)
                     /locus_tag="VGP27_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7853495.1"
                     /translation="MDEPLNSADERPLAGRLDEHLDDVALHTEYRRLVAEQAALRRLG
                     TLVARGGEPSEVSRAVAEEMRRCLDAFTAGMWRFEPTGEVTLVAAAATPAALAKWPVG
                     TRISIEANTLATVVYRTGLPARIDSYENIAGSIPARVRAVGVRAAVGVPIIVDGSVRA
                     LAAVGSAEPGPMPADTEVRISRFAELVASVVVAGYRDEQKRQLLGDVSQRPFLVDSLL
                     EGRVTDRWSLSEAASWLRLPARGPFVVIAAEVPVLGSEALPAVESKLRGLDVYSAWRL
                     QPDLQVGIVHVKSERQLDEILGLVSRLATNRVGVSSCFDDLRDTPQALYFAKVMQRGR
                     PHHSSPVAVFDGSILATAAVADPEVIVKSIANALRGFEDLPEDERETLCETFRVWQDN
                     DASVRRTAEVLFCHPNTVRHRLRRIEKHSGRSLSRPKDVAELCLASEVHRRLM"
     gene            complement(8384..8887)
                     /locus_tag="VGP27_19385"
     CDS             complement(8384..8887)
                     /locus_tag="VGP27_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7853496.1"
                     /translation="MTAITDFDPAAFLVVPARTFEDLRIGEVFRAPSRTLTDAHAAAF
                     QTVSMDNHPVHCDAEWARKHGHVAPVVHGLQVLAFTAPGATLFPHFIGEVFISFLELS
                     CTFLAEVHSGDILYPALTITGLEPNGDDGIVVTAVTVHNQRGQLVLSGQHKYLLRRQR
                     AVQNGDD"
     gene            complement(8983..10044)
                     /locus_tag="VGP27_19390"
     CDS             complement(8983..10044)
                     /locus_tag="VGP27_19390"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEV7853497.1"
                     /translation="MYAMHDTTAPVLITGGSGFLATHIILRLLNEGYRVRTTVRSLDR
                     APEARTTLERAGADTTQLSFVTADLTIDDGWPPAVAGCEYVLHVASPFPPQQPRNADD
                     LVVPAREGTLRVLRAANDAGARRVVLTSSFAAIGYSPKPSGLPYDETDWTDPTDDVTP
                     YVKSKTLAERAAWDFVADPSVGLGLSVVNPVGIFGPALGPDLPTSLGLIQMLMQGKPP
                     LLPRASFAVVDVRDVADLHLRAMLKPEAAGQRFLASAGQPLKLLEIAAILRAALSDGE
                     ARIPTREVPDWVVRTVGRFVPALGTFADLLGPPKAVSAEKATKVLGWQPRSAAVTVAD
                     TGKSLLALRESVADEASPR"
     gene            complement(10028..10501)
                     /locus_tag="VGP27_19395"
     CDS             complement(10028..10501)
                     /locus_tag="VGP27_19395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV7853498.1"
                     /translation="MSIIAEHSDLTIQQVSRHTGLAESALRYYERIGLIGAVPRDESS
                     GHRRYPPDLVAAIEALSCLRGTGMSVQDMRTYVANMRRGLIAAADQRELFAGHADRLR
                     GEIARLRLRERYVSAKAELWAAREQGDAAAEAAVSATVSELAAQLLREEANVRHA"
     gene            complement(11128..11439)
                     /locus_tag="VGP27_19400"
     CDS             complement(11128..11439)
                     /locus_tag="VGP27_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853499.1"
                     /translation="MQPNDGGGSAASSAAGVIPFQRGAPSTSKQVGQIPANYSEGSGT
                     IVSGTEADRATEAALPAYPGGVVDRVVKLSDGEYEVHYIGVNWPHHVFVDQDFNVVGA
                     N"
     gene            complement(12334..12461)
                     /locus_tag="VGP27_19405"
                     /pseudo
     CDS             complement(12334..12461)
                     /locus_tag="VGP27_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         2270 a         4147 c         3792 g         2252 t
ORIGIN      
        1 accggtgtgg aggcggtgct gctgaccttg cgggaggcgg ttgaccggaa ggagttcaac
       61 gacttcatgt cgcagctccc tgtggagttc accaggctgc tcccgtcgcc taatccgcca
      121 cgggcggtat agagcctcca gttcaaagca ggaccctcgc gagctctcgg taaccgggca
      181 tcgggttctc gccgtcggga gtgagcacct gaattccgat gtggtccgcg cctgcatcga
      241 gatgggaatg cacgcccgca gcaacggtgt cggcggagcc atgcagcacg aggccgtcga
      301 tgagacgatc gctgccgccg ccttcgaaat cagcgtcggt gtatccgtac cgccggaagt
      361 tgttggtgta gttcgttagt ttcaaatagg gatcggacac gaaagcgcga ccgaccgtcc
      421 gcgcagtgtc cagctcaagt tgccctccgg acaacctact cggtgcttta tccaatcccc
      481 ttgaggggga acgagtcgag gatgttgcgc ttgcggtccg gcgttcctgg ctcgctcccc
      541 taaagagcat ttgttgcttt gattacaaat gtcacttagt atgaagccgt gctaactttt
      601 gagcaacggg gttcaacaca cgtcgatccc cgtgtgcggc gctcgcgtgc ggcgttgttc
      661 actgccgtgg ccgagctcgt cagcacggcc ggttcgacgg acctgtcgtt agcggccatc
      721 gccgaggccg ccgacgtcag ccgacaggtc atctaccagc agttcggcga ccgcgacacc
      781 ctggtcgtgc agacagccct gggcctgctg cgccacgacg tgcttccaga gatcgccgac
      841 cgtcccaccc tggcaccgcg ggagcgcgca ctggtgactg cgaagcactt cgctgctcac
      901 caccggttct atcgccccgt gttggcgggt tcttgcgcgg cggcgttcag ccgcggcctc
      961 accgagttgc acctgccacc caaccgtgtg ttggtcgacc agattctcgg cgagcaccgc
     1021 gacccgcaga ctgccgaaga cctcgcggtc tacctgaccg gtggcggcat tgcgttcctc
     1081 acggcgtggg ttctgaacgg atcggagcac caggactgcg agcgattcgt cgaccgcctg
     1141 ggctacctgc atgccgcctt gatccgcacc gagggaccgc taccgcgtcc ggaagggacg
     1201 gattcatgag cctggacctg gcgggaaggg tcgcggtcgt gaccgggggc gcctctgggc
     1261 tcggcaagtc catcgtcgaa cggttcgtcg ccgaaggcat gcgtgtcgtc atcgccgacg
     1321 tcaatgacat cgacggcgaa cgactcgcca acctgcacgg ctccgatgtg ttgttccgtc
     1381 ccaccgacgt cgcggtaccc gagcaggtga ccgccctggt agaaacggcg gtcagccagt
     1441 tcgggggttt ggacgtcatg gtcaacaacg ccggcgtcgc cggtacccgg cactgggact
     1501 tcctcgacga cgacctcgcc gacttcgaca gagtcatgtc ggtcaatctg ctgggtgtca
     1561 tggtcggtac ccgcgatgcc gcccgacaca tggccgacca cggcgggggc tcgatcatca
     1621 acatgtcctc catcggggga atcgaggcca gccccggagt gatgccctac ggcgcctcca
     1681 aggccgccgt catccacttc accaaggctg cggcactcgc tctggcgcag tacgagattc
     1741 gcgtcaactg catcgcaccc gggcacatcc gcaccccgct gctggccacc tcggcaccag
     1801 acctatcgcc cgacgacttg gcccagtacg aggctgagat ccgcgccatc atgggcagcc
     1861 aacggcctct gcagcgggaa ggcaaagccg ccgacatcgc aggtgccgcc gcctatttcg
     1921 ccggtgacga ctcggtgtac gtcaccggca ccgtgctacc cgtcgacggc ggagtcgtcg
     1981 ccgggaacac cgccaaacca ccacgattca cacccaaggg agacaagcaa tgaaggtcac
     2041 cgtcgaatcc cgcaagtgca tcgccgcggg catgtgcgtg gcatccgcgc ccgaggtctt
     2101 cgaccaggac gacgacaacg gcatcgtcgt gctgctcgac gaacgacccg gcgccgatca
     2161 gatcgacaac gtccgcgatg ccgccgacgt ctgccccgcg caagtcatct cactgcagga
     2221 ctagatctgc ccccgaggct caggagatcc catgtcggcc accagtgccc accccaccgc
     2281 ccacgaacaa ctcgtcgact atccgatgga acgatcgtcg gcctgcccgt tcgccccacc
     2341 cgagcagctc cgcgaagttg ccgcgcgggc accgatctgc cgcgtgcgga tctgggacgg
     2401 cagcacccca tggttggtca gcgggtacgc ccagatgaag cgactgacct cggatccccg
     2461 ggtcagcgtc gatgacaccc tggcgggctt tccgcactgg aatgccttcg tcgcgcagta
     2521 tcaccggccc cgatcagtgt tcaactccga cggggagcag cactcgcact accggcggat
     2581 gatgacgaag ccgttcaccg tcaagagagt caacgcgctg cgtccggcca tccagcagat
     2641 cgtcgacacc aagatcgacg aactgctcgc cggaccgaac ccagccgatc tggtgaccgc
     2701 gctggcggtt ccggtgccct ccctggtgat cagcgaaatc ctcggcgttc ccgaacagga
     2761 tcgcgcgtac ttcgagcagg aggcgttcca cgctaccgac cacgaagcca ccgtcgagga
     2821 gaagatggcc tccgcccggt ccttgatgga ctacctcaag aatctcgtcg cagccaagat
     2881 ggcgtccccg gccgacgatc acgtatccga actctctcaa cgcgtcgagg ccggggaaat
     2941 ccctctggag gaggcggcct acctcgccac tgggttactc agcgccggcc acgagaccac
     3001 ggcgaacatg atcggcctgg gcaccgcggc cctgctgcag catcccgaac agttggcgct
     3061 gatgcgagac agcatcgaac cggaggccat cgccagcgcc gtcaacgagt tgttgcgcta
     3121 cctcagcatc attcacaacg gccaacgtcg aatcgctctg gcagacatcg acatcgacgg
     3181 cgtgcacatc gccgcggggg agggaatcat cctcgacttg tcaccggcca actgggacga
     3241 gacggtgttc cccgacgccg gacgcctgga tctgcgtcgc cccgccggca accacgtcgc
     3301 cttcgggttc gggccgcatc agtgcgtcgg ccaacaactt gcccgcgccg aactacagat
     3361 cgtctacccg gcgctgctgc ggcgcgtccc ctcgctacgc ctggccatcc cggctgagga
     3421 catcgcgttc atgtaccgcg agctcgcctt cggcgtgtat tcactgccgg tcacctggta
     3481 accccacccc cataaaggac ttcatccgtg acacgcaccg aacacgacac ttgggacacg
     3541 acctccagcg tcggcgccac cgcgaccggc tgcgccgtgg tacgcgcgct ggccagccgg
     3601 cagtcacatc cgttgatcga cgaccccttc gccgccgcgc tcgtgcagca ggccggcgtg
     3661 gacgccttca cccaagtggt cgacgggcaa ctcgcaccct cggacgtctt cggtccgctg
     3721 gccccggcga tcatcgacag catggccgta cgcacggcat ctcacgacta cttcgctctc
     3781 gaccgcgtgg cccagggcac ggcccaggtc gtcatgctgg cttccggatt ggacacacgc
     3841 gcataccgat tggcctggac gtccggtacc accgtcttcg acgtcgatct agaatcggtc
     3901 accacgttca agtccacgac attggccgcg ctcgggttct caccatccag cgaccaccgc
     3961 cccgtcggag cggacctgcg ctacgactgg tccacacgac tcctcgctgc cggcttcgac
     4021 cccggcaagc gcgcggtgtg gctggtcgaa ggccttctcc cctacctccc accggacgct
     4081 caggatgccc tgctggacaa catcaccgga ctctcggcac cagacagcgc cctgatcatc
     4141 gaaagcgtca ccgacgcccg ctcacccgag cacgacgagt tccaggccga attggatcag
     4201 gtgtccgaac ggcaacgcca gcagggccac gacctcgaca tcggcacact catgtacgag
     4261 ggagaccgcc acgaaaccgc cagctacctc aacaccgcgg gctggaccac cacacaggtc
     4321 agcgccaccg aactcttcgc ctcgtgtggt cggcccttgg cacacggtga gcgaccattc
     4381 cccgacggac gcaccgttct cgccaccaag ggctgagatc ccctcagcga gagaccactt
     4441 aacgcctcac cgagcgaaga gggcacacgt ccacgccgcc gtgccgggtg cggtccgtga
     4501 ctggctcgtc acgccactat gggccgtgaa tacgttgtcg cagtacgtgc ccggcggccc
     4561 tgtatcccat accccgtgac caattggcca ttcagtggtc attccagatg ggatttggtc
     4621 acgaagtatg ggaagtcaca ccgccgccgc cggtgtaggt tctcacgttt ggcgtccgtt
     4681 cacagttcgc tgaactgcgc ttgtactccc tgtctcatgc gatgtccgct gcgattcgtc
     4741 tcatccaaat gtccaatttc aagacgtagc ctggcgtcga ctccccagcc ccatcgtccg
     4801 gctgaggagg tctagctgtg agctcaccgc cgtcgcgctg accgtagcgc ccccgccgcg
     4861 gtccctgcgc cgggcccagg cgtcgacgtc acagcggctg gcgtgatcgt gcacgccggc
     4921 gggccggagc ccgatccgtc acctcccgct gcttgacgga gccggcagta aggacattga
     4981 ccattcgaga acaaacgttt ggatatcagc taattattct gcaggatagc ctaattcgat
     5041 cctgtcgcca cccgagaccg gcggcgatcc gctcgagttt gatccatgcc tggtggctgt
     5101 aggaacgaag tcaacctcat gaaccggtgt gcggctggcc attcagcgtt atcaaaagtt
     5161 gctgatctgc attggcgcat cgtgtctcgt ttgacagtga cgggttcctg attgtcgccg
     5221 acggtggagg caggggcgga gagtccgcct tgggtataca tcgaaatgag caccaccatg
     5281 cgactcggat tgaaacacat cgcaccaatg ttgggcgctg cagctgtctc ggcggcaatc
     5341 gcgggcgccc cagttgccag tgccgatccc gaccagtcca atcagactca ggaatcgtgc
     5401 accaacccgg aagcgtcgtc gaccgtatgc caatcgccag gcgatgttga gatcaccgat
     5461 gcgcctccgc ctaggacctt ctatccgtac gggtcgccac cccctacggc tggctgaccg
     5521 cgagcgcgcc gaaacagctt gtgcgaagga aggatcaatg atgggaatcg gacggaaata
     5581 catcgcaccc atgctcggag cgatggcggc cgtggcagcg atcggcacag caccgctagc
     5641 ggccggcgaa gaaccgtcgg acgcgcagca gccaccgccg cctgggcagt cctgcgtgca
     5701 gttgggcacc tcgcaattca agtgcgaatc gcccggcgac gttcagctca acgacgctcc
     5761 atcggtaacc aacaacccgt actgagagct acgcccgtcg gtgggaacgg ttggcgacgc
     5821 cgtatggtgc gtgcgccagg cacgacggac ctggtgattc agcgggtgcc cgcgggtatc
     5881 ggactggcgt ggagcttgcc cgccagggtg tcgcagactc ccaaccgaga tggtgtggta
     5941 aagctcgtgt cacgagttta ccgttgccac aatgctgttt ccggttgcat acgctgccga
     6001 cggcacgcat tcggcgacgt agcggttcac gggcgggccg agggcttgcg cggccagggt
     6061 gacgggctgc cagttaacac ggcgtcgcag ccaatcacca ccgcacagcc gatgtccgca
     6121 agccgccgcg tgagcatcgg ttactggttg gtgtagggcg gccctgacca attcgatcgt
     6181 gtcgcgcagc acggcgtgct tgtcggcgat gacctcgagt ttgacccacg cggtgcccag
     6241 cgcctcgcgc gccagccgcg cgttcagcag cccctcggcg gcgttaggca gcgggtgatg
     6301 cccagccggt tcggcggatc cagcacgcct gtgccgccct cggcatcgag caccgccagg
     6361 ttggccgcac agcaatcgag ttgtccaaag gcagacgccg gcaccgcttc acgaaggcca
     6421 cacatcgact gcatacaagg cccgatgact gccgctactc gaatactcga acacgtaggc
     6481 ggtgtctgcc gtgaccgaca tcaacgtatt gatactcgtc ggactccgtg cggcttctat
     6541 cactcgcgaa ctcgcgaagg tcgcggtcga cagttcagcg ctggcgacgc gtcgaatccg
     6601 gtgtcatcgg ccagactgca gttcggatga cggctgctgg tgaagatcgg gttcagtggc
     6661 ggcctgtgcg cgccggaaca gggcgatggc aatctgattc accgcccccg cgacagagaa
     6721 cacaccaatc gcccacgccc ccatcatgat ctctgcagtg tgtgggcggc caggcagaag
     6781 ccgttcatcg tgctccagtg aatttgatgg atactgctac ggattcactt cgtctatgaa
     6841 ggaacggatt tgtttcatct atgaaggagt ggtcgcacca tcgacttcga cagcgccaca
     6901 cctcattgtg gctcttgctg cgatctcagg cgttgtcgtc cgatacaacg ctgttctggt
     6961 atcggtgtat tcaacaacca tccacatggt tacatgagcc ggcgatgtac ttcggaagcc
     7021 aaacatagtt cggcgacatc ctttggacgc gagagagatc ggccgctgtg tttctcgatc
     7081 cggcgcaatc gatgacgcac ggtgttggga tggcaaaaaa gcacctcggc ggtcctgcgc
     7141 acggatgcgt cgttgtcctg ccacacccga aacgtttcgc acagcgtctc gcgctcgtct
     7201 tcggggaggt cctcaaagcc tctcaaggca tttgcgatcg atttcactat cacctcaggg
     7261 tcagcgactg ccgcagtggc cagaatcgag ccgtcgaaca cggcaaccgg cgaggaatgg
     7321 tggggtcggc cccgctgcat caccttggcg aaatacagag cctgcggcgt gtcgcgcaga
     7381 tcgtcgaaac acgaactgac gccgactcga ttcgtcgcca accgcgacac caaaccgaga
     7441 atctcgtcaa gttgtcgctc agatttgacg tgcacgatcc cgacctgcaa atcgggttga
     7501 agccgccatg ccgaatacac gtcgaggccg cgcagcttgg attccaccgc tggcaatgcc
     7561 tcgctgccca gcaccggaac ctccgcggcg atgaccacga acggacccct ggccggcagc
     7621 cgcagccaac tagctgcctc cgacaagctc caccgatcag tgactcgccc ctcgagcaac
     7681 gaatcaacca ggaacggacg ctgagacaca tcgcccagga gctgcctttt ctgctcatcg
     7741 cggtagccgg ccaccacgac actcgcgaca agttcggcga agcggctgat gcggacctcg
     7801 gtatcggccg gcatggggcc cggttctgcg gaaccgacgg ccgcgagtgc ccggacgctg
     7861 ccgtcgacga tgatcgggac tcctaccgcc gcgcggacgc ccaccgcacg cacccgtgcg
     7921 gggatcgacc cggcaatgtt ctcgtagctg tctatcctcg cgggcagacc ggtgcggtac
     7981 accaccgttg ccaaagtgtt tgcctcgatc gagatgcggg tgcccacagg ccacttggca
     8041 agcgcggccg gagtagcggc agcggccacc agcgtaacct cgccggttgg ctcaaagcgc
     8101 cacattccgg cggtgaaagc atcgaggcac cggcgcattt cctcggccac agcgcggctc
     8161 acctccgacg gttcgccgcc gcgcgcgacc agcgtgccca gccgccgcag ggccgcttgc
     8221 tcggcgacta gccggcgata ctcggtatgc agcgcgacat cgtcgagatg ctcgtcaagt
     8281 cgcccagcaa ggggtcgttc gtcggcggag ttgagcggtt cgtccacgat ttcataccct
     8341 ttcctgctgt tttctggacc acgctaatcg cgcgactggt gttctagtca tcgccgtttt
     8401 gtacggcccg ctgacgccgc agcaggtact tgtgctggcc cgacagcacg agttgcccgc
     8461 gctggttgtg gacggtgacc gctgtgacca cgatgccgtc gtcgccgttc ggctccaggc
     8521 cggtgatcgt gagcgccggg tacaagatgt ccccggagtg cacctcggcc aggaaggtgc
     8581 acgacagctc aaggaagctg atgaacacct cgccgatgaa gtgcgggaac aaggtggctc
     8641 ccggtgcggt gaaagccagc acctggaggc cgtgtacaac cggcgcgaca tgaccgtgct
     8701 tgcgcgccca ctcggcgtcg cagtgcaccg ggtgattgtc catagagacc gtctggaaag
     8761 ccgcggcatg ggcgtccgtc agggtgcggc tgggggcccg gaagacctca ccgatgcgca
     8821 ggtcctcgaa cgtcctggcc gggacgacca ggaacgctgc gggatcgaag tcggtaatag
     8881 cagtcatcgc gctctttctg ctctggagat gattctggac aactcgttta ccaagatggg
     8941 gagcgctggc attcatccgc gttgatctgc gacgacattc gtctagcgtg gcgacgcctc
     9001 atcggcgacg ctttccctta gcgccagcag gctcttgccg gtatcggcca ccgtaacggc
     9061 ggcagaacgc ggttgccagc caagtacttt ggtggccttc tcggctgaca ccgccttagg
     9121 agggcctagc aggtcggcga acgtacccag cgctgggacg aaacgtccca cggtacgcac
     9181 cacccagtcc ggcacctctc gcgttggaat ccgcgcctcg ccgtcgctca acgcggcgcg
     9241 caggatcgcg gcgatctcaa gcaatttcag tggctgaccg gccgatgcca ggaatcgttg
     9301 ccccgccgcc tctggtttca gcatggcccg tagatgcaga tcggcaacgt cacgcacgtc
     9361 gacgacggcg aacgacgccc gcggcagcag cggcggtttg ccttgcataa gcatctggat
     9421 taggccgaga gaggtgggca ggtccggccc gagggccggc ccgaagatgc cgacagggtt
     9481 caccaccgac agtcccaggc cgaccgacgg gtccgcgacg aaatcccatg ccgcccgctc
     9541 cgcaagcgtc ttgctcttga cgtacggcgt cacatcatcc gtcgggtcgg tccagtccgt
     9601 ttcgtcgtat ggaagccctg acggcttcgg gctgtagccg atcgcggcga acgaagacgt
     9661 cagcaccacg cgccgcgccc cagcgtcatt ggcagcccgc aacacccgca gcgtgccttc
     9721 gcgtgcgggc acgacgaggt cgtccgcgtt ccgcggttgc tgcggcggaa acggcgacgc
     9781 cacgtgcagc acatattcgc agccggcaac cgcgggcggc catccgtcat cgatcgtgag
     9841 atcggcggtg acaaaagaca gttgtgtcgt gtcggcgccc gcgcgttcca gcgttgtgcg
     9901 cgcctctggc gcacggtcga gcgaccggac agtggtgcgt acccgatatc cctcgttgag
     9961 tagccgcaga atgatatgtg tggcaaggaa tccggagccg ccggtgatca acaccggcgc
    10021 ggtggtgtca tgcatggcgt acattcgcct cttctctcaa caactgggcg gcaagttcgc
    10081 tcaccgtcgc gctgacggcc gcctccgcgg cggcgtcgcc ctgttccctg gcggcccaga
    10141 gctcggcctt ggccgacacg taccgctcgc gtagtcgtag ccgggcgatc tcgccccgaa
    10201 gccgatccgc atggccggcg aagagctctc gctggtccgc cgccgcgatc aagccgcgcc
    10261 gcatgttggc gacgtaggtg cgcatgtcct gcaccgacat tccagtgccg cgtagacagc
    10321 tcagcgcttc gatggcggcg acgagatcgg gcggatagcg gcgatgacca ctgctctcgt
    10381 cacgggggac cgcgccgatt agcccgattc gctcgtaata tcgcagcgcg gactccgcca
    10441 gtccggtgtg ccgtgacacc tgctggatgg tgaggtccga atgctcggcg atgatgctca
    10501 tgccaccact atcccgaact tgaagcgctt caagtcaagg cgtggtcact cgcacttaca
    10561 cgctgatctc gcgtactccg atcagaatca aacgctagcg cggctagata ccatgctcga
    10621 taccaataaa gaacacctca cgcgggcttc gtgcggctga gaattttgcc gcgccaactg
    10681 agcgttactc catctcgatc ccgtagtcga gattgtggtc gcggctgcgg ctgctaccca
    10741 gattctcact ttcgtgtcca atcggacatc gggtggacat ttggtatttg atgtccaaac
    10801 gcgatgcgtt gaactgcact taggtagctc gtttcacgtg atgtccagcg cgaaacgtct
    10861 catcttcgtg tccacttcaa tcgcagctca tcgtcgctgc cggaggcccc gagcggtgct
    10921 cgaccgtgcg gtcgcgcaca ctttgacatt cgtcacggtg gcgcctggat gtcagtccga
    10981 aatctcaccc aaatgccctg agagtttggc cctattattc tggccctata gatttgatct
    11041 tgaagcctgt gcccatcagg cgagacgcgg cggtgcgaac cgcaacgaat cggcagcgga
    11101 tacgttggaa cccgcaagag caatagacta attggcaccg acgacgttga agtcctggtc
    11161 gacgaagacg tggtgcggcc agttgacgcc gatgtagtgg acctcgtact cgccgtcact
    11221 cagcttcaca acacggtcga cgacgccccc agggtaggcg ggcagcgcag cttccgtcgc
    11281 cctatccgct tccgttccgc tgacgatcgt ccccgacccc tcactgtagt tcgccgggat
    11341 ctgaccgacc tgcttcgacg tgctcggtgc accgcgctgg aaggggatca cccctgccgc
    11401 cgaggaagcc gcagatccgc cgccgtcatt cggctgcacg atgacctgcg tggccgtgat
    11461 ggtcgttccg ctggtcgtcc caagtacgag aacactttcc cctgttgtaa tcgcactcgc
    11521 tgaggtcgag ctcgtcccgt tcaggtatgt cgtagaggac gcctcgttga cggtcacctt
    11581 ctgacccgcc gatgtcgaca ttgtgaagct cgacgcagac acgctgtcga tcgtgccgga
    11641 tgcaccccct gaggccgctc cagaccgagc gttggctccc ccgctgccgg gcacgggctg
    11701 ccctgatgtt gccgcggagg acgcggtcgt tgccgccggg gacgcggtcg ttgccgaggg
    11761 ggacgtggcg caaccggcag aaaatccgat caccgcacac atcagcgcag cggccaagat
    11821 ccctccactt tctctgttcg acaccaatcg ccgtacccct ggccggcgtg cctgcttggc
    11881 catggttacc gaagccaacg agtctggcgg aggaggcggc gaaacggggc gagcggaaag
    11941 ggtctcgtgg ggacttgttg ggtcgtaatc gttcatgacg gcgctcctcc tacatgggca
    12001 acaacgcaga gcgtgccaac cgttcatttg agatcgatgt aggcggcctg tgctgggact
    12061 gtgactgctc ctgcggactc gccggtcttc acctaagaca agcaagccat ttcgagcagc
    12121 tgagcaatcg aaatcgtcgg atgtactgct cgtgcgtatt tctctgtggc acactgaggt
    12181 tcacccttgc gggcgagtga ttcgtaggtt tcgtcgccga ggacttcgcg caggtgggcg
    12241 atcgcagtgc ttagctcagg gatctaagcc ctgctgaggt ggccgaacgt agtcgaaccc
    12301 gttgcgccgg ttcacgtctt agccggtgtt cccgcggccg tgtgcacgcc aaccgccgcc
    12361 gtcgggtatg cggcccagct tcttgacgtc catgtgcacc agttcgcctg gccggggtcg
    12421 ttcataacga acggcggtgg ccttagaggc gcggatcacc t
//
LOCUS       DASYLX010000826         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_706074,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000826 DASYLX010000000
VERSION     DASYLX010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706074"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VGP27_19410"
     CDS             <1..1390
                     /locus_tag="VGP27_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853500.1"
                     /translation="SSGLQFSGSTATFVASLATLTMAAAVLSAGALGDLYGMRRMYLI
                     GLAGAIAFSALAAAAPMPAVLMIARAGVGITLAFLIGLSLAITNAVFPPGRRAAAIAA
                     YFGVGYAVATPLPALSGALAHAISWRACFFVVPLIAAVGLVITWRYVPETMRANRRLD
                     LPGLALFAIALLGLIFGTSRMETGIDNVGLASIAVGVAAGCGFVFQERRTPDPALDMC
                     VFRSGRFNAAVTAGVVFNIVLGGSMVLLAFYLVTIRKESHELFGLLLIPATAMAALSA
                     TAAGPAANRFGARTVLVGGLTTMLAGLLVLRSFDLHTSRPVVFVTTALIVVGGALVTT
                     PQATIMMGSAPARLGGAVSAVKSAVNEGGYSLGPALFALVGINLFLTDSVHDLSRSGI
                     TRAETREALLTVHGGRGAHLVNPEQARLVVDQAPQNAVDAIHTLSLIMAVGPIAAIIL
                     ALLLIKPSAETN"
     gene            1482..1619
                     /locus_tag="VGP27_19415"
     CDS             1482..1619
                     /locus_tag="VGP27_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853501.1"
                     /translation="MSDKSPRQAMTKKSGKSLKEKRAAKHAKTAAKASSAETLFHDKK
                     R"
     gene            1603..2796
                     /locus_tag="VGP27_19420"
     CDS             1603..2796
                     /locus_tag="VGP27_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /protein_id="HEV7853502.1"
                     /translation="MTRSADTQQLSLGVLARSHKPNERRLPIHPAHFSRIDPRVRRRI
                     ILEHGYGSDFGASDESLAGLVGGIRTREQVVADCDVILLPKVQAEDLTELTSGQVVWG
                     WPHCVQDFALTQEAIDRRLTLIAFEAMNHWQADGGFGLHVFHKNNELAGYCSVLHAMQ
                     LVGITGSYGRRLRAAVIGFGATARGAVTALNAHGVDDVRVLTNRDVAAVGSPIPSTQI
                     IQMGPDPDNPDRIWADTPDGSVPVARLLADNDIIVNCVLQNPAAPLVFVTEADLASFT
                     PGSLIVDVSCDAGMGFSWARPTSFTQPLIAVANGLHYYAVDHSPSYLWNSATWDISEA
                     LLPHIGPVLAGPASWEKTPTIARAIEIRDGVVLNPAILSFQHRDEAYPHAVRDPLNQP
                     VSALS"
     gene            complement(2812..>3582)
                     /locus_tag="VGP27_19425"
     CDS             complement(2812..>3582)
                     /locus_tag="VGP27_19425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7853503.1"
                     /translation="TPHQLSLLAWIGATADQVVVMSEVAKQRLCAGYGVDRRKVTTIP
                     YGAVVPSGPPTKRPSRPTVLTWGLMGPGKGIERVIEAMSSLNHLPGRPRYLVVGPTHP
                     KVLAEDGEAYRDALTEQVRRSGIADSVVLDSRYFSGPMLTALVQSANVVVLPYDSTDQ
                     VSSGALADAVASGRPVVATAFPHAVELLCNGAGVIVDHDDPGALVAALRSILTQPRLA
                     GSMAAEARRLAPEMAWPVVAAAYLRLAQRLIADRPVVI"
BASE COUNT          578 a         1225 c         1151 g          628 t
ORIGIN      
        1 ctccagcggc ttgcagttct ccggcagcac cgccaccttc gtcgccagcc tggccaccct
       61 caccatggcc gccgccgtgc tcagcgccgg cgcgctgggc gatctgtacg gcatgcgacg
      121 catgtacctg atcggactgg cgggcgcgat cgcgttcagc gcgctggccg ccgccgcgcc
      181 gatgccggcg gtgttgatga tcgcccgcgc cggtgtgggg atcaccctgg cgttcctgat
      241 cggcctgtcg ctggcgatca ccaacgcggt cttcccaccc ggccgacggg cggcggccat
      301 cgccgcgtat ttcggggtgg gatacgccgt cgccaccccg ctgcccgcgc tcagcggcgc
      361 gctcgcgcat gcaatcagtt ggcgcgcgtg ctttttcgtc gtgccgctga tcgccgcggt
      421 ggggttggtc atcacctggc gctatgtgcc cgagacgatg cgcgccaacc gccggctgga
      481 tctgcccggc ttggcgctgt tcgcgatcgc gctgctggga ctgatcttcg gtacctcgcg
      541 gatggagacc ggcatcgaca acgtcggtct cgcctccatc gcggtgggcg tggcggccgg
      601 ttgtggcttc gtattccaag agcgccgcac gccggatccg gcgctggaca tgtgcgtctt
      661 ccgctcgggc cggttcaatg cggccgtcac cgccggagtg gtgttcaaca tcgtgctcgg
      721 cgggtcgatg gtcctgctgg cgttctatct cgtcaccatt cgcaaggagt cgcatgagct
      781 cttcgggctg ctcttgattc ctgccaccgc gatggcggcg ctgtccgcga ccgccgcagg
      841 ccctgccgcg aaccgcttcg gcgctcgcac ggtgctcgtc ggcgggctca ccaccatgct
      901 cgcgggcctg ctggtactga ggtccttcga cctgcacacg tctcgaccgg tggtctttgt
      961 caccacggcg ctcatcgtgg tcggcggtgc actggtcacc acaccgcagg ccacgatcat
     1021 gatgggcagc gctccggctc gcctcggcgg tgccgtctcc gccgtcaaga gcgctgtcaa
     1081 cgaaggcggt tactcgctcg ggccggccct cttcgcactc gtgggcatca acctcttcct
     1141 caccgacagc gtgcacgacc tgtccagatc cgggatcacc cgagcggaga cgcgcgaggc
     1201 gttgctgacg gtccacggcg gccgcggtgc ccatctggtc aacccggagc aggcacgact
     1261 ggtggtcgac caggcgccgc agaacgcggt cgacgcgatt cacacgttga gtttgatcat
     1321 ggccgtcggg ccgattgcgg caatcattct ggcgctgttg ctgatcaagc cttcggccga
     1381 gaccaactaa cccgtgcacg cccgggtccg cagcgccgac gacaggagta gggtcagagt
     1441 gaatcgggac caaccaggcc cccacgatca aggggccctc gatgtcggat aagtctcctc
     1501 gacaagccat gaccaagaag tccggaaagt ctctcaagga gaagcgcgcc gccaagcacg
     1561 ccaagacggc ggccaaggcc tcgtccgccg agactctgtt ccatgacaag aagcgctgac
     1621 acacaacagc tttcgttagg cgttctggct cgctcgcaca agccgaacga acgcagattg
     1681 ccaatccatc cggcacattt cagccggatc gatcctcgcg tccggcggcg gatcatcctc
     1741 gagcatggct acgggtcgga tttcggcgcg tcggacgagt cgctggccgg tctcgtcggt
     1801 ggaatcagga ctcgggaaca agtcgttgcc gactgcgacg tcattctgct accgaaagtt
     1861 caggcagagg atctcacgga actcacttcg ggacaggttg tctggggctg gccgcactgc
     1921 gtgcaggatt tcgccctgac gcaggaggcg atcgatcgcc ggttgacgct gatcgctttc
     1981 gaggcgatga accactggca agccgacggc gggttcggcc tgcacgtctt tcacaagaac
     2041 aacgagctgg cgggctactg ttcggtcctg cacgcgatgc aactggtagg catcaccggc
     2101 agctatggcc gccggttgcg tgctgccgtc atcggattcg gcgcgacagc tcgcggcgcc
     2161 gtcaccgccc tgaatgccca cggcgtcgac gatgtgcgcg tactgaccaa ccgggacgtg
     2221 gccgccgtcg gttcgccgat tccttccacc cagatcatcc agatgggacc cgaccccgac
     2281 aatcccgacc ggatctgggc ggacaccccg gacgggtccg ttccggtggc acgcctgctc
     2341 gccgacaacg acatcatcgt caactgcgtc cttcagaatc cggcggcacc attggtcttc
     2401 gtcaccgaag cggatctggc ctccttcacc ccgggaagcc tgatcgtcga cgtctcctgc
     2461 gacgctggca tgggtttcag ctgggcgcgc ccgacctcgt tcacgcagcc cctcattgcc
     2521 gtggcgaacg ggctgcacta ttacgcggtg gaccacagcc cgtcgtacct gtggaactcg
     2581 gcgacctggg acatcagcga ggcattgtta ccccacatcg gtccggttct ggccggcccg
     2641 gcgagctggg agaagacccc gacgatcgcc agggcgatcg agatccgcga cggcgtcgtg
     2701 ctcaatcccg ccatcctgtc gtttcagcat cgcgacgagg cctacccgca cgcggtccgc
     2761 gacccgctca accagccggt gagcgccctc tcctagctga ggtggtatcg gtcagatcac
     2821 tacaggccgg tcggcgatga gccgttgcgc cagccgcaga tatgcggcgg ccaccacggg
     2881 ccaggccatt tccggcgcca accggcgcgc ctcggcggcc atcgacccgg cgaggcgcgg
     2941 ctgagtcagt atgctgcgca acgccgccac cagcgcaccg ggatcgtcgt gatcgacgat
     3001 gacgcccgct ccgttgcaga gcagctcgac agcgtgtggg aaagcggtgg ccacgactgg
     3061 ccggccgctg gcgacggcgt cggccaacgc gccagaactg acctggtcgg tggagtcata
     3121 gggcagcaca acgacgtttg cagactgaac cagggcggtc agcatcggcc cgctgaagta
     3181 gcgggagtcc aagaccacag aatccgctat tccgctgcgg cgcacctgct cggtcagcgc
     3241 gtcgcggtac gcctcgccat cctcggcgag gactttcggg tgcgtcggtc cgacgacgag
     3301 gtagcgcggc cggccgggca gatgattcag agaactcatc gcctcgatga cgcgctcgat
     3361 gcctttcccc ggccccatca ggccccatgt caagacggtt ggtctactcg gacgcttggt
     3421 cggaggcccg ctcggaacga cagcgccgta cgggatggtc gtgaccttgc ggcgatcgac
     3481 gccataaccg gcacacaacc gttgcttcgc aacctccgac atgacgacca cttggtcggc
     3541 tgtggcgccg atccaagcga gaagcgaaag ttgatgcggc gt
//
LOCUS       DASYLX010000827         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_267218,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000827 DASYLX010000000
VERSION     DASYLX010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267218"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGP27_19430"
     CDS             <1..571
                     /locus_tag="VGP27_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853504.1"
                     /translation="TLNIVLIEVGAGDLSGAGAALASVTQVGPVVTAIVVSGAGATAM
                     CADLGARTIREEIDAMEVIGVNPVQALVVPRVIAATFVALMLYSVVAVVGLFGSYFFV
                     VFVQHVTPGAFVAGMTLLTGLPQVVVSLVKALLFGLSAGLIACYKGLHVGGGPTAVGT
                     AVNETVVFAFMALFLINILATAFGVKVAP"
     gene            580..1416
                     /locus_tag="VGP27_19435"
     CDS             580..1416
                     /locus_tag="VGP27_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853505.1"
                     /translation="MDDSSPVEQAVAKAVGRIRTLGEQTAFYGQALWNTPDAVRRYST
                     MVLRQIAGMGMGTGALAVIGGTVVIIGFLTLSTGALIAVQGYNTLSNVGIEALTGFLG
                     AFLNVRFIAPATAGVALAATIGAGATAQIGAMRINEEIDALEVMGIRVITYLASTRIV
                     AGVVAVIPIYTVAVLMSFLATRFGTTIIYGQSRGVYDHYFDTFLQPTDLLWSFVEALA
                     MAAAVMAIHTYYGYTVTGGPAGVGEAVGRAVRLSITAGVFILLTITLSVYGQSGNFHL
                     SG"
     gene            1424..>2369
                     /locus_tag="VGP27_19440"
     CDS             1424..>2369
                     /locus_tag="VGP27_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853506.1"
                     /translation="MTNSTRREKIDPIWWAPVLLIVVAAVSTLTAMLFSGTLHDYQPL
                     TLVSDRSGLVMEDGAKVKLRGVQVGEVTNIGAETGAAGDVSKLTLKIDPEPFKYLPSN
                     LEAEIKSSTAFGAKYVDLIVPADGASPDRLKPGSVVRSRNVTVEVNTVFQSLQGVVQS
                     IDPAKLNSILSAVAESVRGKGDAIGAAITDSNNVLSAVNPRMETVRQDWQLFGRTTKA
                     YSDAAQDIVSILDSFSTTSTTISDNAQVLDTLLLSAVGFSDAGINTIGANQPNLVRAM
                     NILDPTTQLLMKYSPSYTCLFQGAQWFLEHGGRDAIGGN"
BASE COUNT          385 a          763 c          775 g          446 t
ORIGIN      
        1 cacgctcaac atcgtgctga tcgaagtagg cgcgggtgac ctgtccggtg ccggtgcggc
       61 gctggcctcg gtgactcagg tgggtcccgt ggtcaccgcg atcgtcgtct ccggcgcggg
      121 cgccaccgcg atgtgcgccg acctcggggc ccgcaccatc cgggaagaga tcgacgcgat
      181 ggaggtcatc ggggtcaacc cggtccaggc tctcgtcgtg cctcgggtga tagcggcgac
      241 tttcgttgcg ctgatgctct attcggtcgt cgcggtggtg ggcctgttcg gaagctactt
      301 cttcgtggtc ttcgttcagc acgtcacacc gggcgcgttc gtcgcgggga tgacgctgct
      361 gaccgggctg ccgcaggtgg tcgtctcgct cgtgaaggcg ctgctgttcg ggctctcggc
      421 cgggttgatc gcctgctaca agggtctgca cgtcggtggc ggcccaacgg cggtgggcac
      481 cgccgtcaac gaaaccgtcg tcttcgcctt catggcactg tttctcatca acattctggc
      541 caccgcgttc ggggtcaagg tggcaccgtg acctcagagg tggacgactc gtcgcccgtc
      601 gagcaagcgg tggcaaaggc ggtcggccgc atccgcaccc tcggggagca gacggccttc
      661 tacgggcagg cgttgtggaa cacccccgac gccgtccggc gctattcgac aatggtgctg
      721 cgccagatag cgggcatggg gatgggaacc ggggcgctgg cagtcatcgg cggcacggtc
      781 gtcatcatcg gatttctcac gctgtccacc ggtgccctga tcgccgtgca gggttacaac
      841 accctgtcga acgtcggtat cgaagccctc accggctttc tcggggcctt tctcaacgtc
      901 cggttcatcg caccggcgac cgcgggcgtg gcactggccg ccaccatcgg cgccggtgcc
      961 actgcccaga tcggcgcgat gcgcatcaac gaggagatcg atgcgctgga ggtgatgggc
     1021 attcgggtga tcacctacct tgcctccacc cgcatcgtgg ccggcgtggt ggcggtcatc
     1081 ccgatctaca ccgtcgcggt gttgatgtcg tttctggcca cccgattcgg caccacgatc
     1141 atctacgggc agtcgcgagg cgtatacgac cactacttcg acacgttcct gcaaccgacg
     1201 gacctgttgt ggtcgttcgt cgaggcgctg gcgatggccg ccgcggtgat ggccatccac
     1261 acctattacg gctacaccgt gaccggtggc cctgccggtg tcggcgaggc cgtcggccgt
     1321 gcggtgcggc tgtcgatcac cgcaggcgtc ttcatcctgt tgaccatcac gctgtccgtc
     1381 tacggacagt ccggcaactt ccatctttcg ggatgacggg ccgatgacca actcgacccg
     1441 tcgcgaaaag atcgatccca tctggtgggc gccggtcctg ctgatcgtcg tcgccgcggt
     1501 cagcacactc acggccatgt tgttctccgg cacgctgcac gactaccaac cgctgaccct
     1561 tgtttccgac aggtcgggtc tggtgatgga ggacggcgcg aaggtcaaac tgcgtggagt
     1621 gcaggtcggc gaggtcacca acatcggcgc cgagaccggc gcggccggcg acgtgtcgaa
     1681 actgacgctg aaaatcgacc ccgagccgtt caagtacctg ccgagcaatc tggaagcgga
     1741 gatcaagtcg agcaccgcgt tcggcgctaa gtacgtcgac ctcatcgtgc ccgcagacgg
     1801 cgccagcccc gatcggctca agcccgggtc ggtggtgcgc tcccgcaatg tgaccgtcga
     1861 ggtcaacacc gtgttccaga gcctgcaggg cgtagtgcag tccatcgacc cggccaagct
     1921 gaactcgatc ctgtcggcgg tggccgaatc ggtgcgcggc aagggcgatg cgatcggagc
     1981 agcaatcacc gattcgaaca acgtgctgtc cgccgtgaat ccgcggatgg aaaccgtccg
     2041 gcaggactgg cagctgttcg gccggacgac gaaggcgtat tcggatgccg cgcaggacat
     2101 cgtgtcgatc ctcgattcgt tctcgacgac gagcaccacc atcagcgaca acgcgcaggt
     2161 gctggacacc ttgttgttgt cggcggtcgg cttctccgac gcgggcatca acacgatcgg
     2221 cgcgaaccag ccgaacctgg tgcgcgccat gaacatcctc gacccgacga cccagttgct
     2281 gatgaagtac tcgcccagct atacctgcct gttccagggc gctcagtggt ttttggagca
     2341 cggtggccga gatgcgatcg gcggcaacg
//
LOCUS       DASYLX010000828         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1143495,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000828 DASYLX010000000
VERSION     DASYLX010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143495"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGP27_19445"
     CDS             <1..221
                     /locus_tag="VGP27_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEV7853507.1"
                     /translation="AINGCSQTLYHPVGTCRMGRDDASVVDPQLRVRGVDGLRVADAS
                     VMPAIVRGHTHAPSVMIGEKASDLIRGG"
     gene            complement(222..629)
                     /locus_tag="VGP27_19450"
     CDS             complement(222..629)
                     /locus_tag="VGP27_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEV7853508.1"
                     /translation="MNLEQFFQRATQAFEVLGVSSMVLGTGYAIGLAAFTWRRTGGAR
                     AFKTLRNAIGGAILLGLELLVAADIVKTVTSKPSLTDAAILGVIVLIRTVLSFTIEVE
                     IDGVAPWRKAATTGPEVLARTTRTARSPSGPGV"
     gene            691..1944
                     /locus_tag="VGP27_19455"
     CDS             691..1944
                     /locus_tag="VGP27_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853509.1"
                     /translation="MTMNAKRRRAHDKLAALPGVRPVRRPVSPDRDDEFDLYYVRSGR
                     KSMTPVVVIPGGPGAASVALYRGLRRHAAARGIDVIMIDHRGVGMSRHDDRGADLPPE
                     ALTIEQAVQDIAAVLDDAQVDKAVVYGTSYGTYLAAGIGVRHPNRVHAMVLDSPLLSA
                     HDIDAVRDETRRVLWDGNEPEAAELAPKVRKLVDAGILTPAAGQLTAAVYGLAGPALL
                     TRQLDLLLRGRRGLWTVLDQAARLLFERKAPYRHEPDLVGRIGFRELNYGAIPDGKPL
                     DPAVAFREFAAAPLEFEAEPYDLAAEMPKFRWPTAVISGGRDLITPRAVAEEIVSLIP
                     DSVLVELATAGHSALDVRERAALDIVEAVVDGDHSGLPARADELDAVPAGLGVRLLVG
                     ALGAAAVVESVLPAVIPRVVQRVTS"
     gene            1991..2614
                     /locus_tag="VGP27_19460"
     CDS             1991..2614
                     /locus_tag="VGP27_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853510.1"
                     /translation="MTNIPQAMESPVPLREFIVSPGFAGAAAVVAALIVFCAVLYASR
                     RAGKRLNEDLEQRERHHHEARDAEKQALASQQCWETLKWLVKTAGLEPAASEAATLGL
                     GPELAMATLRGLLRDAERLGDETLADAVTVYQNQFALILAQQGGPLSALAASASPAVG
                     GRSPQPRPASTEEPAKAAEVQPDRQSAPADESSTSTIEVASGRRRRQ"
     gene            2623..>3644
                     /locus_tag="VGP27_19465"
     CDS             2623..>3644
                     /locus_tag="VGP27_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853511.1"
                     /translation="MRHIADLSAEKWAALTRRAAAEAVAAAERRGKTPPPELVAVAAM
                     TERQLVEHRARNGPARQQLSPLMQLVEADHMRRVAEAGARQANQARLDAEAAAAAARA
                     EAEESSRTAAAAREHARTVHQQAAQKETERTTERAAAAEAHERGVQQLRSELEHVRAD
                     AEAELAAAAERATAAEARAQQRVTERTAASEAAEQAVQEWRAELEKVRADADAEVAAG
                     RERAAAAETRAQQRADERTAASEAAERAAAQLRGELVRVRADADAETAASRGWAAGEV
                     AAVREAADAEVARAYAAADEAIRQAQTRAARTLSMPVPPFEFRSETAHIENALNALHQ
                     IDYVLEV"
BASE COUNT          609 a         1220 c         1287 g          528 t
ORIGIN      
        1 cggcgatcaa cgggtgttcg cagacgctct accaccctgt cggcacctgc cggatgggtc
       61 gcgacgacgc gagtgtggtg gacccgcaac tacgggtgcg cggcgtcgac ggtctgcggg
      121 tcgccgacgc atccgtgatg cctgcgatcg tccgcgggca cacccacgct cccagtgtga
      181 tgatcggcga gaaggcttcc gacctcatcc gcggcggcta gctagacacc gggaccggac
      241 ggcgatcggg ccgtgcgtgt ggtccgcgcc agcacctcgg gaccggtcgt ggcggccttg
      301 cgccacggcg ccactccgtc gatctccact tcaatggtga agctgagaac cgtgcggatg
      361 agcacgatca ccccgaggat ggctgcgtcg gtgagcgacg gcttcgacgt gacggtcttg
      421 acgatgtcgg ccgcgaccag cagttcgagg ccgagcagga tcgcgccgcc gatcgcattg
      481 cgcagtgtct tgaaggcgcg tgctccgccg gttcgcctcc atgtgaatgc ggcgaggccg
      541 atggcatagc cggtgcccaa caccatcgac gaaacgccca acacctcgaa cgcctgcgtc
      601 gcccgctgga agaactgctc caagttcact gcgtccccat cgtcgacctg ttccgcgact
      661 ctattaccgg cgggggcaga atggtcgacg atgacgatga acgccaaacg tcgccgtgcg
      721 cacgacaaat tggccgcttt gccgggcgtg cggccggtgc ggcgacccgt atcgcccgac
      781 cgggatgacg agttcgacct ctactacgtc cgttccggcc gcaagtcgat gactccagtg
      841 gtcgtcattc ccggcgggcc gggggcggcg tcggtcgcgc tgtatcgagg gctgcgcagg
      901 cacgccgcgg ccaggggcat cgacgtcatc atgatcgacc accgcggcgt tggcatgtcc
      961 cgccacgacg accgcggcgc cgatctgccc cccgaagccc tcaccatcga gcaggccgtg
     1021 caggacatcg ccgccgtgct cgacgacgcc caggtcgata aggcggtcgt ctacggaacc
     1081 tcgtacggca cctatctggc cgcgggaatc ggtgtgcgcc atccgaatcg ggtacatgcg
     1141 atggttctcg actcgccgct gctttcggcg catgacatcg acgccgtgcg agacgaaacc
     1201 cgacgcgtgc tctgggacgg gaacgagccc gaggccgccg aactggcgcc gaaggtgcga
     1261 aagctcgtcg acgccggaat tctcactccc gcggcgggac agctgactgc agcggtctac
     1321 ggtctcgccg gaccggcgct gttgacccgc caactcgacc ttctgctgcg gggccgacga
     1381 ggattgtgga ccgtcctcga tcaggccgca agactgctgt tcgagcgcaa agcgccgtac
     1441 cgccacgagc cggacttggt cggccgcatc ggttttcgtg aattgaacta cggtgcgata
     1501 ccggacggca agccgctgga ccccgccgtc gcattccgcg aattcgccgc cgcccccttg
     1561 gaattcgaag ccgagcccta cgacctcgca gccgagatgc cgaagttcag gtggcccacg
     1621 gccgtcatct ccggtggccg ggacctgatc acgccgcggg cggtcgccga ggagatcgtg
     1681 tcgttgattc ccgattcggt tctggtcgag ctggcgacgg cgggtcatag tgcgctggac
     1741 gtccgcgagc gggcggcgct cgacatcgtc gaggccgtgg tcgacggcga ccattccggc
     1801 ctgcctgccc gcgccgacga actggacgcc gtgccggccg gcctgggcgt ccggctgttg
     1861 gtcggggcgc tcggcgcagc ggcggtcgtg gagtccgtat tgcctgccgt gattccgcgg
     1921 gtggtgcagc gggttacttc gtgaacccga tcgcgctggc cgaggtcgcc gatacggcag
     1981 aatgcttcgg atgaccaata ttccgcaggc aatggagtct ccagtgccgc tgcgcgagtt
     2041 catcgtctcc ccgggcttcg ccggtgcggc agcagtggtc gcggcactga tcgttttctg
     2101 tgctgtgctt tacgcgagca ggcgggcggg caagcggctc aatgaggacc tcgagcagcg
     2161 cgagcgccat catcacgaag cgcgcgatgc cgagaagcag gctttggcct ctcagcagtg
     2221 ctgggagacg ctgaagtggc tggtcaagac cgcaggcctg gaaccagcag ccagtgaggc
     2281 agccactctc ggcctcggtc cggaactcgc gatggcgact ctgcgaggat tgcttcgcga
     2341 cgccgagcgg ctgggagacg aaaccctcgc cgacgccgtg acggtctatc agaatcaatt
     2401 cgctctcatc ctggcgcagc agggcgggcc gctttccgca ctcgccgcga gcgcctcccc
     2461 ggcggtgggc ggacgaagcc ctcagccgcg gccggcttcg acggaggagc cggccaaggc
     2521 cgcggaggta cagcccgaca ggcagtccgc gccggccgat gaaagttcca cgtcgacaat
     2581 tgaagtggcg agcggacggc gtcgtagaca atgacgccga ccatgcggca tatcgcggac
     2641 ctcagcgccg agaagtgggc cgcgctgacc cgtcgggctg cagcggaggc ggtggcggcc
     2701 gctgaacgac gcggaaagac gcccccgcca gagttggttg ccgtcgcggc gatgaccgag
     2761 cgtcagctcg tcgagcaccg cgcacgaaat gggccggcgc gacaacagct ttcgccgctc
     2821 atgcaactcg tagaggccga ccacatgcgg cgcgtggcgg aagccggggc acgtcaggcg
     2881 aaccaggcga ggctggacgc cgaggcggca gccgcggcgg ccagagccga agccgaagag
     2941 tcatcacgca ctgccgctgc ggcgcgagaa cacgcccgca cggtgcacca gcaggctgcc
     3001 cagaaagaaa ctgaacgaac caccgagcgc gcggccgccg cagaagcgca cgaacggggt
     3061 gtgcaacagc tgcgcagcga gctggagcac gttcgcgccg atgccgaagc cgaactggcg
     3121 gcggccgccg agcgcgcgac ggcggcggag gcccgcgccc agcagagggt cacggagcgc
     3181 acggccgcaa gtgaggccgc cgagcaggcg gtgcaggaat ggcgcgccga gctcgagaag
     3241 gtgcgcgccg atgccgacgc cgaggtcgcg gcgggccgtg agcgggctgc cgcagcggag
     3301 acgcgtgccc agcagagggc cgatgagcgt acggccgcaa gtgaggccgc cgaacgggcg
     3361 gctgcgcagt tgcgcggcga gctcgtgcgc gttcgcgccg acgccgacgc cgagaccgcc
     3421 gcatcccgcg gatgggctgc cggtgaggtg gcggccgtcc gggaggccgc cgacgccgag
     3481 gttgcgcgcg cctatgcggc tgccgacgag gcgatccggc aagcacagac ccgggcggcg
     3541 cgcacgctgt cgatgccggt ccctcccttc gagttccgtt ctgaaaccgc gcatatcgag
     3601 aacgcgctca acgcgttaca ccagatcgac tacgtgctgg aagt
//
LOCUS       DASYLX010000829         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3448958,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000829 DASYLX010000000
VERSION     DASYLX010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448958"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGP27_19470"
     CDS             <1..348
                     /locus_tag="VGP27_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853512.1"
                     /translation="GYRLSGGQRQRLTIARLLLGRSRVVVLDEATASLDSASEAAVQQ
                     ALAEALAGRTSIVIAHRLSTVRAADTILVVEGGRIVERGDHEQLLSSRGRYAELYETQ
                     FGKQADAEDVVAA"
     gene            427..>1733
                     /locus_tag="VGP27_19475"
     CDS             427..>1733
                     /locus_tag="VGP27_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853513.1"
                     /translation="MRSAPAVARPPARRRTSSDLWRLLPYLMPYRVRWIAMISVALLS
                     LVATVAIPLMTKAVIDGPVRHQDQHGLWVLGAAAMGIGISEAVLWFIRRWLVARGTMG
                     VEADIRKDLYARLQILPMSFHGRWQSGQLLSRIMNDLSTIRRFMSFGMVFLLLNVIQI
                     TVVTAILLAMYWPLGVVVLISIVPIGATVLHFQQEYTRLSRLAQDQSGHVATHVEESA
                     LGLRVVKSFGREDYVYDRFDEQLTHLYDTQVRRVSVSAKFWTLLEIIPNLTLIVVLGL
                     GAYAAGHGYVTMGTLVAFITMMLSLVWPIASLGFLLSMTQESFTAANRIAEIFDAPRE
                     ITDGPEAQTPQGGRLELVDVGFRFPDAKVDDLALRHVNVTVEPGETLALVGATGSGKS
                     VLASLLSRLYDVTEGEIRIDGQDIRELSLPALRQAVATAFEDPT"
BASE COUNT          262 a          565 c          588 g          318 t
ORIGIN      
        1 ggctaccggc tctccggcgg ccagcgccag cggctgacca tcgcacggct gctgctcggc
       61 cgatcgcggg tcgtggtgct cgacgaggcc accgcgtcgc tggactcggc ctcggaggcg
      121 gccgtgcagc aggcactcgc cgaggcactc gccggacgga cctccatcgt gatcgcgcac
      181 cgcctgtcga ccgtccgtgc ggccgacacg atcctcgtcg tcgagggcgg ccgcatcgtc
      241 gagcgcggcg accacgagca gctgctgtcg tcgagggggc gctatgcgga gctctacgag
      301 acacagttcg gtaagcaagc ggacgccgag gatgtcgtag cggcgtgaga gcctggaacc
      361 aaccgatatt gatttgccgc tcagcggata attggactca ctttgactga accaaaagta
      421 gacgcgatgc gctcggcccc cgcggtggcg cggcccccgg ctcgcaggcg caccagctcg
      481 gatctgtggc ggctgttgcc ctacctgatg ccgtaccgcg tgcgctggat cgcgatgatc
      541 tccgtcgcgc tgctgagcct cgttgccacc gtggcgatcc cgctgatgac caaggcggtg
      601 atcgacgggc ccgtgcgtca tcaggatcag cacggcctct gggtgctcgg cgcggcggcg
      661 atgggcatcg gcatctccga ggcggtgctg tggttcatcc gtcggtggct ggtggcccgc
      721 ggcacgatgg gcgtggaagc cgatatccgc aaggatttgt acgccaggct gcagatcctg
      781 ccgatgtcgt tccacggtcg ctggcagtcg gggcagctgt tgtcgcgaat catgaacgac
      841 ctgagcacta ttcgtcggtt catgtcgttc ggcatggttt tcctgctgct caacgtcatc
      901 cagatcaccg tcgtgacggc gatcctgctg gcgatgtact ggccgctggg tgttgtcgtg
      961 ctcatctcga tcgtgccgat cggcgcgacg gtgctgcact tccagcagga gtacacccgg
     1021 ctgtcgcgat tggcgcagga ccagtccggt cacgtcgcga cgcacgtgga ggagtccgcg
     1081 ctcggcctgc gggtggtcaa gtcgttcggc cgggaggact atgtctacga ccggttcgac
     1141 gaacagctca cccacctcta cgacacgcag gtccgtcgtg tctcggtgtc ggcgaagttc
     1201 tggacgctgc tggaaatcat cccgaacctg acgctgatcg tcgtgctcgg tctcggcgcc
     1261 tacgcggcgg gccacggcta cgtcacgatg ggcacgctcg tcgccttcat caccatgatg
     1321 ttgtcgctgg tctggccgat cgcctcgctc ggctttctgc tgtcgatgac gcaggagtcg
     1381 ttcaccgccg ccaaccggat cgccgagatc ttcgacgcgc cccgcgagat caccgacggg
     1441 cccgaggcgc agacgccaca gggcggccgg ctcgaactgg tcgatgtcgg attccggttt
     1501 cccgatgcga aggtcgatga cttggcgctg cggcatgtca atgtcacggt ggaaccgggc
     1561 gagacgctgg ccctcgtcgg cgcaaccgga tccggcaaat cggtgctggc cagcctgctc
     1621 tcgcggctgt acgacgtgac cgagggcgag atccgcatcg acggccagga catccgcgaa
     1681 ctgtcgctgc ccgcgctgcg acaggccgtg gcgaccgcgt tcgaggaccc gac
//
LOCUS       DASYLX010000830         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_485159,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000830 DASYLX010000000
VERSION     DASYLX010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485159"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VGP27_19480"
     CDS             complement(<1..170)
                     /locus_tag="VGP27_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853514.1"
                     /translation="MSFSLELSEDVIHVRDWVHEFAESVLRPAAAEWDEREETPWPII
                     QEAAKVGLYSVD"
     gene            483..929
                     /locus_tag="VGP27_19485"
     CDS             483..929
                     /locus_tag="VGP27_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7853515.1"
                     /translation="MPRLSLASRLSRRWMLLVAVSVVAIAGFAVYRLNGIFGSHDVTS
                     TPSGAANDIVPFNPKHVVLEVFGPPGTVATITYLDVNAQPQRADDATLPWAYDTTTTQ
                     PAVFVNVSAQGDSDSIGCRIKIDDVVKDERTVNTLNAFTYCLDKSG"
     gene            926..>1530
                     /locus_tag="VGP27_19490"
     CDS             926..>1530
                     /locus_tag="VGP27_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853516.1"
                     /translation="MTHHVAERPTFTDRAATWIRWLCVPIVLFWVAVAALTNVLVPQL
                     EAVGEQHNVALSSPDSPSLQAFQRIGKVFGEFDTDSAAMVVLEGDKPLGADAHAYYDE
                     LVKRFDEDTKHVQHVQDFWGDPLTAAGSQSPDGKAAYVQVFLAGNQGEALSLESVDAL
                     RDIIAKTPAPQGIHAYVAGSAAQIADQFEVGNQSTVMVTALT"
BASE COUNT          289 a          510 c          463 g          268 t
ORIGIN      
        1 aagtccacgg agtagaggcc cactttggcc gcctcctgga tgatcggcca aggggtttcc
       61 tcgcgttcat cccactccgc tgccgccgga cgcagtacgg attccgcgaa ttcgtgaacc
      121 cagtccctga cgtgaattac atcctcggaa agctcgagag aaaacgacac agccgctcac
      181 ccttcttatg cacacgcacg ggagtagtac caacttcggt gaacaaccgt acactgatac
      241 ttaccaggga gtaagttcag ccactatgga gtaggtcact cgcggccggc cacgggcaag
      301 tctgatgcgc cgcactctcc tcaacgatcg aagggcgccg ttcccggcat cgcccggcac
      361 aatgcctgag gggtatttgt tgtggcgtcg ggtaccgtct cgtactgtca acctgattga
      421 tatagggccg acccgtagaa acattgccgt ctgcaggtcg ccgctcgtcg aaagggtcgg
      481 ggatgccgcg gctttcgctc gcaagccgat tgagccgccg ctggatgctt ctggtggcgg
      541 tgtccgtcgt cgcgatcgcg ggattcgccg tgtaccgcct caacggcatc ttcggctcgc
      601 atgacgtcac gtccaccccc agcggcgccg ccaacgacat cgtgccgttc aaccccaagc
      661 acgtcgtcct ggaggtcttc ggcccaccgg gcacggtcgc gaccatcacc tacctggacg
      721 tgaacgccca gccgcagcgg gccgacgacg ccacgctgcc gtgggcctac gacaccacca
      781 ccacccaacc ggccgtcttc gtcaacgtct ccgcccaggg cgacagcgac tccatcggtt
      841 gccgaatcaa gatcgacgac gtcgtcaagg acgagagaac ggtcaatacg ctgaacgcct
      901 tcacctactg cctggacaag tccggatgac ccaccacgtc gccgaacgcc cgacgtttac
      961 ggatcgcgcc gcgacgtgga ttcgttggct ctgtgtgccg atcgtattgt tctgggtggc
     1021 cgtcgccgcg ctgacaaacg tgctggtgcc gcagctcgaa gcggtcggtg aacaacacaa
     1081 cgtggcgttg agctcccccg attcgccctc gctgcaagcg tttcagcgca tcggcaaggt
     1141 tttcggtgag ttcgacaccg acagtgcggc gatggtggtg ctcgagggtg acaagcctct
     1201 cggcgccgac gcgcacgcct actacgacga gctggtcaag cgcttcgacg aggacaccaa
     1261 gcacgtccag cacgtacagg acttctgggg cgatccgctg acggccgcgg gctcgcagag
     1321 cccggacggg aaggccgcgt acgtccaggt gttcctcgca ggcaaccagg gcgaggcgct
     1381 gtcgctggag tcggtggacg cgctgcgcga catcatcgcc aaaacgccgg caccacaggg
     1441 tattcacgcg tacgtcgccg gctcggcggc ccagatcgcc gaccagttcg aggtgggcaa
     1501 ccagagcacc gtgatggtca ccgcgttgac
//
LOCUS       DASYLX010000831         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1252562,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000831 DASYLX010000000
VERSION     DASYLX010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252562"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGP27_19495"
     CDS             complement(<1..386)
                     /locus_tag="VGP27_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7853517.1"
                     /translation="MDELRKKGLEKMNEVYGWEMPDMPGEYFALTVDHLFGAIWSRPG
                     LSMRDRRMMLLGVLAAQGMEELIEVQANAILHNEEMTREELREVAIFLTHYVGFPLGS
                     KVDGAVKRVVAKRKKAEEKGSARDSK"
     gene            complement(392..1267)
                     /locus_tag="VGP27_19500"
     CDS             complement(392..1267)
                     /locus_tag="VGP27_19500"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853518.1"
                     /translation="MGDEQVKLGYIGLGDMGAPMAKRMVDWPGGLTVFDIRLEAMTPL
                     AEAGAALADSLADVAKADVISVTVLNDEQVRDVIAGEDGLARYAKPGTIVAIHSTISD
                     TTAVDLARKLAPDGIHVIDAPISGGKGAAEKGELAVMVGAQREVYERVKPVFKQFASV
                     VVHAGDVGAGTRMKLARNMLTFIGFAASCEAMKLAEQAGLDLQQLGRVVRHTDIQSGG
                     PGAVVVRENMDTLEPDNFLYGPMAHARDLGEKDLSLALALGESVGVDLPLAEIALKNL
                     AAGLGVPHAAASAKE"
     gene            complement(1278..>1749)
                     /locus_tag="VGP27_19505"
     CDS             complement(1278..>1749)
                     /locus_tag="VGP27_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853519.1"
                     /translation="GGMKLDFLITVDWDYYKKFMSVNLDGALVCTRAVYRKMAKRGGG
                     AIVNQSSTAAWLYSNFYGLAKVGINGLTQQLATELGGQNIRVNAIAPGPIDTEANRTT
                     TPKEMVDDIVRRIPLSRMGEPEDLVGMCLFLLSDQAKWITGQIFNVDGGQIIRS"
BASE COUNT          277 a          602 c          534 g          336 t
ORIGIN      
        1 tccttgctgt ctctggcgga acccttctcc tcggctttct tacgcttggc cacaacgcgt
       61 ttgaccgcac cgtcgacctt cgaccccagc gggaagccga cgtagtgcgt taggaagatc
      121 gcgacctcac gcaattcctc gcgggtcatc tcctcgttgt gcaggatggc gttcgcctgg
      181 acctcgatca actcctccat gccctgtgcg gccagcacgc cgagcagcat catccgccgg
      241 tcgcgcatcg acagcccggg gcggctccag atggcgccga agaggtgatc gaccgtcaac
      301 gcgaagtact cccccggcat gtccggcatc tcccagccgt agacctcgtt catcttttcg
      361 aggcccttct tgcgcagctc gtccatgact actactcctt cgcgcttgcc gccgcgtggg
      421 gcacgccgag gcctgccgcc aagttcttca gcgcaatctc ggcgaggggc aggtcgacgc
      481 cgaccgactc accgagcgcc agggccaggc tgaggtcttt ctcgccgagg tcgcgggcgt
      541 gcgccatcgg cccgtagaga aagttgtccg gctccagcgt atccatgttc tcccggacga
      601 cgacggcgcc cggcccgccg ctctggatgt cggtgtgccg caccacgcgg ccgagttgct
      661 gcaggtcgag tccggcctgc tcggcgagct tcatcgcctc acaggatgcg gcgaaaccga
      721 tgaacgtcaa catgttgcgc gccagcttca ttcgcgtgcc ggcaccgaca tcgcccgcgt
      781 gcaccacgac ggaggcgaac tgtttgaaga ccggcttcac ccgctcgtag acctcgcgct
      841 gcgcaccgac catcacggcg agttccccct tctccgcagc tcccttgccg ccgctgatcg
      901 gagcatcgat cacgtggatg ccgtccggtg cgagcttgcg ggccaagtcg acggcggtgg
      961 tgtcgctgat cgtcgagtgg atcgcgacga tcgtgcccgg ctttgcatag cgggccagcc
     1021 cgtcctcgcc agcgatgacg tcacgaacct gctcgtcgtt cagcacggtg acgctgatga
     1081 cgtcggcctt cgcgacgtcg gcaaggctgt cggcgagcgc ggcgcctgcc tcggcaagcg
     1141 gagtcatcgc ctcgaggcgg atgtcgaaaa ccgtcagacc gcccggccag tcgaccatgc
     1201 gtttggccat cggtgcgccc atgtcaccga gcccgatgta accgagcttg acctgttcgt
     1261 cacccattga attctcctca ggacctgatg atctggccgc cgtcgacgtt gaagatctgg
     1321 ccggtgatcc acttcgcctg atccgacagc aggaacagac acatcccaac cagatcctcc
     1381 ggttcgccca tgcgcgacag cggaattcgg cgcacgatgt cgtcgaccat ctccttgggc
     1441 gtcgtcgtgc ggttggcctc ggtgtcgatc ggcccagggg cgatcgcgtt gacacggatg
     1501 ttctggccgc cgagttcggt ggccaactgc tgggtcaggc cgttgatgcc gaccttggcc
     1561 agtccgtaga agttcgagta cagccacgcc gcggtcgacg actggttaac gatcgcgccg
     1621 ccgccgcgct tggccatctt gcggtagacg gccctggtgc acaccagcgc gccgtcgagg
     1681 ttcacgctca tgaacttctt gtagtagtcc cagtcgacgg tgatgaggaa gtcgagcttc
     1741 atgccgccg
//
LOCUS       DASYLX010000832         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_375791,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000832 DASYLX010000000
VERSION     DASYLX010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375791"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGP27_19510"
     CDS             complement(<1..318)
                     /locus_tag="VGP27_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEV7853520.1"
                     /translation="MPRIAYLGPEGTFTEAALIRMTDGGLIPGGGPSDGFTPLPADST
                     TAALAAVRSGDADHACVPIENSIEGSVLPTLDALASGAELQIFAELTLDVAFTIVVRS
                     GATA"
     gene            432..1223
                     /locus_tag="VGP27_19515"
     CDS             432..1223
                     /locus_tag="VGP27_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEV7853521.1"
                     /translation="MAAPAVTTTPTTAERIRSTCASAGAAMLAIEGAEPTASAVHHLL
                     DDGSFAITVPVDGGPSPASAAGVPAVLEMTDYAPLPLREPVRSLVWIRGLVQAVPHSE
                     ITGLLDLVAAADPNPALLQVNTTDTAGGDTRLTLVRLEVESVVVADSTGAEAVGVSTL
                     LQARPDPFCGMESCWLQHLEAAHRDVIDRLADRLPTTLRRGRVRPLGLDRYGVRLRVE
                     NDEGDHDVRLAFARPVDDVTGLSQAIRILMGCPFLNGLRARRPLP"
     gene            1229..1993
                     /locus_tag="VGP27_19520"
     CDS             1229..1993
                     /locus_tag="VGP27_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV7853522.1"
                     /translation="MTAPEELTEPDRRALRIEIAVILAVTFGLSAYTASLDLIESILL
                     NLSKQTVALNPRLSPFDLINLGLNLARVAQLVSWGALGAYLLWRSGIGLSAIGLGRIR
                     WRADVLGGVGLAALIGLPGLGLYAASRALGMSVSVVPSTHDNSWWHIPVLILLAFANG
                     WAEEVVVVGFLLTRLQQLRVNPVVALVASSLLRGAYHLYQGWGAGLGNAVMGLVFGYT
                     WRRTGRLWPLIIAHGLIDTAAFVGYILLAGHLGWLK"
     gene            2006..3202
                     /locus_tag="VGP27_19525"
     CDS             2006..3202
                     /locus_tag="VGP27_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV7853523.1"
                     /translation="MLPVTGDRRPPPPRRREPSQVIRRDPNRPPPAWPPNPPTPQAPP
                     RRAHPPPPPPPHRRPNPVVRQQSPPAQRPVGRRRLVRRVALTLFTVIVLAVVGLVGTA
                     VWIDTKLHRIPALADYPNRPAEGKGTTWLLVGSDSRQGLTPAQQAELGTGGDIGNGHT
                     DTILLVHIPALWSDSPTTMVSIPRDSYVEIPDYGTDKINAAFAVGGAPLLAQTVEQAT
                     GLRLDHYAEVEFDGFARLVDAIGGVTMCPPEAISDPLAGIDLSAGCQKLDGRTALGYV
                     RTRATPRADLDRMVNQRQFMSTLMHRAASPAVVANPLRWYPMAQAASDSLTVDTGVHV
                     TDLARLAWALRGDLTTTTVPIGEFTGSDSGSVVVWDEDVANQLFTALKSDTPVPQDVL
                     DAGAIH"
     gene            complement(3199..>4003)
                     /locus_tag="VGP27_19530"
     CDS             complement(3199..>4003)
                     /locus_tag="VGP27_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV7853524.1"
                     /translation="FDGHPFVVAHRGASAARPEHTRAAYELALQEGADGVECDVRLTR
                     DGHLVCVHDRRVDRTSNGTGLVSEMSLAELKALDYGSWHASWRADGTQGDTGLLTLDD
                     LVSMVLDWHRPVKIFIETKHPVRYGALVESKVLALLHRYGIASPASADLSRAVVMSFS
                     AAAVWRIRRAAPMLPTVLLGETSRYLGGSAATTVGATAVGPSIATLREHPELVDRAAA
                     QGRALYCWTVDHYEDVRYCRELEVAWVATNHPGRTKDWLQNGLTGAGRD"
BASE COUNT          595 a         1431 c         1351 g          626 t
ORIGIN      
        1 cgcggtggcg ccggagcgca ccacgatggt gaaagccacg tcgagggtga gctccgcgaa
       61 gatctgaagc tccgccccgg acgcgagcgc gtccagcgtc ggcagcaccg acccctcgat
      121 ggagttctcg atcggcacgc aggcatggtc ggcgtcgccg gaccgcactg cggccagcgc
      181 agccgtggtg ctgtcggccg gtagcggcgt gaacccgtcc gacgggccgc caccaggtat
      241 caagccgccg tccgtcatgc ggatcagcgc cgcctcggtg aaggttccct ccggtccgag
      301 gtaggcgatg cgcggcacga atcaacccta tcggttcggc tcctgctgcc ctgccgacgt
      361 cctcgcgtgc ctcttgcaga ccgagtaagg cttcgctaag ttagggtcac cttattcagg
      421 aaggcgatgc gatggcggca ccggcagtga cgacgacacc gacgacggcc gagcggatcc
      481 gcagcacctg cgcgagcgca ggggcagcga tgctcgcgat cgagggtgcc gaaccgaccg
      541 ccagtgcggt gcaccacctt ctcgacgacg gctcgttcgc gatcaccgtc ccggtcgacg
      601 gcggcccgtc ccccgcgagc gcagccggcg tgcctgcggt gctggagatg accgactacg
      661 cacctcttcc gctgagggag cccgtgcgat cgctggtgtg gatccggggc ctggtgcagg
      721 ccgtgccgca ttcggaaatc accgggctac tcgacctggt cgccgctgcg gatcccaatc
      781 ccgcattgct gcaggtgaac acaaccgaca ccgccggcgg cgacacccgg ctcaccctgg
      841 tccggctcga ggtcgagtcc gtcgtcgtcg ccgactccac cggggcggag gcggtcggtg
      901 tcagcacact gcttcaggcg cggcccgacc cgttctgcgg gatggaatcc tgctggctgc
      961 agcatctcga ggccgcccat cgcgacgtga tcgaccggtt ggcggaccgg ttgccgacga
     1021 ctttgcggcg cggacgcgtc cggccgctcg gcctcgaccg atacggcgtg cggttgcggg
     1081 tcgagaacga cgagggcgat cacgacgtgc ggttggcgtt cgccagaccc gtcgacgacg
     1141 tcaccgggct cagccaggcg attcgaatcc tgatgggctg cccgttcctc aacgggcttc
     1201 gggcgaggcg cccgctaccg tagcttcggt gaccgcgccc gaggagttga ccgaacccga
     1261 ccggcgcgcg ctgcgcatcg agatcgccgt catccttgcg gtcacgttcg gcctcagcgc
     1321 ctacaccgcg agccttgatc tgatcgagtc gatattgctc aatctgtcca agcagacggt
     1381 tgcgctcaac ccacggctgt cccccttcga cctgatcaat ctcgggctga acctggcccg
     1441 ggtcgcgcag ctggtctcat ggggagcgct gggcgcatac ctgctgtggc gcagcggtat
     1501 cgggctgtcg gcgatcggcc ttggccgcat ccggtggcgc gccgacgtgc tcggcggcgt
     1561 cggcctcgcc gcgctgatcg ggctgcccgg cctcgggctc tatgccgcgt cgcgcgcctt
     1621 gggcatgagt gtgtcggtgg tgccgtcgac gcacgacaat tcgtggtggc acatccctgt
     1681 cctgatcctg ctggccttcg ccaacggctg ggccgaggag gtggtcgtcg tcgggttcct
     1741 gctcacccgg ctgcagcagc tacgggtgaa tcccgtcgtc gcgctggtgg ccagcagcct
     1801 gctgcgaggc gcgtatcacc tctatcaggg ctggggcgcc gggctcggga acgcggtgat
     1861 gggcctggta ttcggctaca cgtggcggcg caccgggcga ctgtggccgc tgatcatcgc
     1921 gcacggcctg atcgacaccg ctgcgttcgt cggatacatc ctgctggcag gccatctcgg
     1981 ctggctgaag tgacgggccg cgtacattct tcctgtgacc ggcgatcggc ggcctccgcc
     2041 ccctcggcgg cgtgagccgt cgcaggtgat caggcgggac ccgaatcggc caccgcctgc
     2101 ctggccgccc aacccgccga cgccgcaggc gccaccgaga cgggcccatc cgccaccgcc
     2161 tccgccgcca cacagacgac cgaatcccgt tgtgcgccaa caatcgccgc cggctcagcg
     2221 gccggtcgga cgcaggcgcc ttgtccgtcg cgtcgccctc accctgttca cggtgatcgt
     2281 tctggcggtc gtgggactgg tcggcaccgc agtctggatc gacacgaagc tgcatcgcat
     2341 cccggcgctg gccgactacc cgaaccgtcc cgccgaaggc aagggcacca cctggctgct
     2401 cgtcggctcg gacagcaggc aggggctcac cccggcacag caggccgaac tcggcaccgg
     2461 cggcgacatc ggcaacgggc acaccgacac gattctgctc gtgcacatcc cagcgctgtg
     2521 gtcggacagc cccaccacga tggtttcgat accccgtgac tcgtacgtgg agatccccga
     2581 ctacggcacc gacaaaatca atgcggcgtt cgccgtcggc ggcgcgccac tactggccca
     2641 gaccgtcgag caggccacgg gtctgcgact cgaccactac gccgaagtcg aattcgacgg
     2701 tttcgcaagg cttgtcgatg ccatcggcgg ggtgacgatg tgcccgccgg aagccatctc
     2761 cgacccgctc gcgggcatcg acctttcggc ggggtgccag aagctggacg gccgcaccgc
     2821 actgggctac gtgcgcaccc gagcgacgcc gcgggcagac ctcgaccgga tggtgaacca
     2881 gcgccagttc atgtcgacgc tgatgcaccg cgcggccagc ccggccgtcg tggccaaccc
     2941 gctgcgctgg tatccgatgg cccaggcggc gtcggactcg ctgacggtcg acacgggtgt
     3001 tcacgtcacc gacctcgccc ggctggcgtg ggcgctgcgc ggtgacctca ccacgaccac
     3061 cgtgccgatc ggcgaattca ccggcagcga ctcgggttcc gtcgtcgtgt gggacgagga
     3121 cgtcgcaaac caattgttca ccgcgctgaa gtcggacacg cccgtcccgc aggatgtgct
     3181 cgacgctggg gcgatccact agtcgcggcc cgctcccgtc aggccgttct gcagccagtc
     3241 cttggtccgc ccaggatgat tggtggccac ccatgccacc tcgagctcgc ggcagtaccg
     3301 gacgtcctcg tagtggtcga cggtccagca gtacagcgcg cggccctggg cggccgcccg
     3361 gtcgacgagc tctgggtgtt cgcgcagcgt ggcgatcgac ggtccgacgg cggtggcgcc
     3421 caccgtggtg gccgcgctgc cgccgaggta acgcgacgtc tcgccgagca ggacggtggg
     3481 cagcatcggc gccgcgcgcc gaatccgcca gaccgccgcg gccgagaagg acatcaccac
     3541 cgcgcgggag aggtccgccg acgcaggcga tgcgatgccg taccggtgca acagcgccag
     3601 caccttgctc tccaccaacg cgccgtaacg caccggatgt ttggtctcga tgaagatctt
     3661 caccggccgg tgccagtcca gcaccatcga caccaggtcg tcgagagtca gtagaccggt
     3721 gtcaccctgc gtgccgtcgg cccgccaact ggcgtgccac gagccgtagt ccagggcctt
     3781 caattcggcc agcgacatct cgctgaccag cccggtgccg ttcgacgtgc ggtccacgcg
     3841 gcggtcgtgc acgcatacca gatgcccgtc ccgggtcagc cggacgtcgc actccacgcc
     3901 gtcggcgccc tcctgcaggg ccagctcgta cgcggcccgg gtgtgctcgg ggcgggccgc
     3961 cgacgcgccc cggtgtgcca ccacgaaggg gtgaccgtcg aac
//
LOCUS       DASYLX010000833         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_925565,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000833 DASYLX010000000
VERSION     DASYLX010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925565"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1239
                     /locus_tag="VGP27_19535"
     CDS             67..1239
                     /locus_tag="VGP27_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853525.1"
                     /translation="MTTAEVSDEDFHEILAQTRHFVRTAVVPREQEILAGDRVPDDLR
                     DQAAKMGLFGYAIPQEWGGLGLNLAQDVEMAIELGYTSLALRSMFGTNNGIAGQVLVG
                     FGTDAQKAQWLERLATGEVASFALTEPGAGSNPSGLRTKAVRDGAQDDADWIINGDKR
                     FITNAPIADLFVVFARSRPADDDGPGIAVFLVPADAAGVQVGAKDAKMGQEGAWTADV
                     SFTDVRVPAGALIGGSEDIGYRAAMTSLARGRVHIAALAVGIAQRALDESVAYAATAT
                     QGGKPIGSFQLVQAMLADQQTGVMAGRALVRDTARLWLSGEDRRIAPSAAKLFCTEMA
                     GKVADLAVQVHGGSGYMREVPVERIYRDVRLLRLYEGTSEIQRLIIGGNLVKGAQK"
     gene            complement(1315..2172)
                     /gene="purU"
                     /locus_tag="VGP27_19540"
     CDS             complement(1315..2172)
                     /gene="purU"
                     /locus_tag="VGP27_19540"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEV7853526.1"
                     /translation="MSKDVGRLLLRCADRPGLVAAVSSFLADSGANIVSLDQHATEQS
                     GGTFMQRTIFHMPGLTAARDSLEHHFREQVAGRFEMDFRLTEAAKPKRVAILVSKEDH
                     CLLDLLWRNRRGELDMSVSMVISNHPDLADAVRPFGVPFLHVPAANDKRTDAERRQVG
                     LLRGNVDLVVLARYMQILSQDFLTDVGCPLINIHHSFLPAFIGAAPYRRAKERGVKLV
                     GATAHYVTEELDDGPIIEQDVVRVDHRHSVEDLRRLGADVERVVLSRAVLWHCQDRII
                     RFGNQTVVF"
BASE COUNT          495 a          831 c          815 g          454 t
ORIGIN      
        1 atcatatttc atatatgatt tgcggcaccg cgcggcaccg attcgtcgaa caggagtacg
       61 cctgaaatga ccaccgctga agtcagcgac gaggatttcc acgagatcct tgctcagact
      121 cgtcatttcg ttcgcaccgc ggtcgtgccg cgtgagcagg agatcctcgc cggcgaccgg
      181 gtccccgacg acctgcgcga ccaggccgcg aagatgggcc tgttcggcta cgcgatccct
      241 caggagtggg gcggtctcgg cctgaacctg gcgcaggacg tcgagatggc gatcgaactg
      301 ggctacacct cgctcgccct gcgctcgatg ttcggcacca acaacggcat cgcgggccag
      361 gtgctcgtcg gcttcggcac cgacgcgcag aaggcccagt ggctcgagcg cctggcgacc
      421 ggcgaggtgg cgtcgttcgc cttgaccgag cccggcgcgg gatccaaccc atctggcctg
      481 cgcacgaagg cggtccgcga cggcgcacag gacgacgcgg actggatcat caacggagac
      541 aagcgtttca tcaccaacgc gcccatcgcg gaccttttcg tcgtcttcgc ccgcagcagg
      601 cccgccgacg acgacggccc cggcatcgcg gtcttcctgg ttcccgccga cgccgccggt
      661 gtccaggtcg gcgcaaagga cgccaagatg ggccaggagg gtgcatggac ggccgacgtc
      721 agcttcaccg acgtcagggt gcccgcaggc gcactgatcg gcggcagtga ggacatcggt
      781 taccgggcgg cgatgacgtc gcttgcccgc ggccgggtgc acatcgctgc gctggcggtc
      841 ggcatcgcac agcgagccct tgacgaatcc gttgcgtacg cggccacggc cacccagggc
      901 ggcaagccga tcggcagctt ccaactggtt caggcgatgc tggccgacca gcagaccggc
      961 gtgatggccg ggcgcgcact cgtccgcgac accgcccgac tttggctctc cggcgaggac
     1021 cgcaggatcg cgccgtcggc ggccaagctg ttctgcaccg aaatggcggg caaggtggcc
     1081 gatctcgcgg tgcaggtgca cggcggtagc ggctacatgc gagaggtgcc cgtggagcga
     1141 atctatcgcg acgttcggct gctgcggttg tacgaaggaa ccagcgaaat tcaacggctg
     1201 atcatcgggg gcaatctcgt gaaaggcgcc cagaagtgag ccagaggctt gtccgcagaa
     1261 gtcttttcgc tcctcgcgtc cgcagaagtc ttttcgctcc tcgcgtccgc agcatcagaa
     1321 caccacagtt tggttgccga accgaatgat tcggtcctga cagtgccaga gcaccgcgcg
     1381 cgacagcacc acgcgttcca cgtccgcgcc gagccgcctc aaatcctcga cggaatgccg
     1441 atggtcgacc cgcaccacgt cctgctcgat gatcggtcca tcgtcgagtt cctcggtgac
     1501 gtaatgcgcg gtcgccccga ccaacttcac accccgttcc tttgcccgtc ggtacggtgc
     1561 cgccccgatg aacgcgggca gaaacgagtg gtggatgttg atcagcggac aaccgacgtc
     1621 cgtcaggaag tcctgactga ggatctgcat gtagcgcgcc aggaccacca ggtcgacgtt
     1681 gccgcgcaga agaccgacct gacgacgttc ggcgtctgtc cgcttgtcgt tcgctgcggg
     1741 gacatgaagg aacggcaccc cgaaaggccg aaccgcgtca gcgagatcgg gatggttgga
     1801 gatcaccatc gagacggaca tgtccagctc accacgacgg ttgcgccaca gcagatccaa
     1861 taggcagtgg tcttccttcg ataccaggat cgcgacgcgt ttcggtttgg cggcttcggt
     1921 cagccggaag tccatctcga accggccggc gacctgctcg cgaaagtggt gctcgaggga
     1981 gtcgcgtgcg gctgtcagac ccggcatgtg aaagatcgtg cgttgcatga aggtgccgcc
     2041 cgattgttcg gtggcgtgct gatccagcga aacaatgttg gcgccggaat ccgccagaaa
     2101 gctgctcact gccgcaacca agccgggacg gtccgcgcac cgcagcaaca accggccgac
     2161 atccttggac atccctcagg gtctcactcc ggcacgggac ggacagccga ctgaggtgga
     2221 aaaatcagcg gtttggcggc acgatgggac ataggcgctc gctacctggc cgccgaggca
     2281 actgacgacg acctcggccc ggattttcgg caacgttggt tgctcgcggc agtttcgctg
     2341 actcgttcgc cgttcgatcc aaaaaacatc catagtctgc gtatatttac gtgcacgaaa
     2401 gttgcccagc aatttccttc tttcgtacgc gcaaacaaca tagctcgcca agggaatctt
     2461 tgccggacaa gatcggcccg ctagctggaa taacggtctt gaaaagagca atactcgatg
     2521 tctgggtatt catatagacc gcatgtgaat gccgcctcgg taaccgcgac tactcgtcca
     2581 ttctggtcgc cgtca
//
LOCUS       DASYLX010000834         5195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_485577,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000834 DASYLX010000000
VERSION     DASYLX010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485577"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4962
                     /gene="pks13"
                     /locus_tag="VGP27_19545"
     CDS             <1..4962
                     /gene="pks13"
                     /locus_tag="VGP27_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309915.1"
                     /note="Pks13 is a key enzyme in mycolic acid biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase Pks13"
                     /protein_id="HEV7853527.1"
                     /translation="RFPGDMNTPEEMWQALLAGRDAITDLPDGRWVEFLEEPTLAARV
                     HKARTRGGYLSDIKGFDAEFFALSKMEADNIDPQQRMALELTWEALEYARIPASSLRG
                     TSVGVYIGNSTNDYSFLAMADPSTAHPYAITGTASSIIANRVSYFYDFRGPSMAVDTA
                     CSSSLVAVHQGVQALRSGEADVVLAGGVNALVTPLVTIGFDEIGGVLASDGRIKSFSQ
                     DADGYARSEGGGMLVLKRVADARRDGDEIMAVIAGSAINHDGRSNGMLAPNPDAQAEV
                     LRKAYKDAGIDPRTVDYIEAHGTGTILGDPIEADALGRVVGRGRASDKPALLGAVKSN
                     VGHLESAAGAASLAKMTLALRNDKVPPSINYTGPNPYIDFDAIHLKVADTVTDWPRYS
                     GHAVTGVSGFGFGGANAHLVLREVLPSDLVEPEPETEDHSEGMHAAPRGDATYVGGVR
                     MDEYGEFIDDDENEGAEFFGQHAYDTDERDPGLPGLTDEARRLLEVAREEAEAEEPHT
                     PVVPLAVSAFMTSRKKVAAADLADWMDSEEGRGSSLESIGRSLSRRNHGRSRAVVMAH
                     DHDEAIKALRALADGKQSPNVYSADGPVTNGPVWVLAGFGAQHRKMAKSLYLRDEVFA
                     EWINTVDALVQDELGYSVVELILDDSQDYGIETTQVTIFAIQIALGELLKHHGAKPGA
                     VVGQSLGEAAAAYFSGGLSLADATRTICSRSHLMGEGEAMLFGEYIRLMALVEYSAEE
                     IKTVFSDYPDLEVCVYAAPTQTVIGGPPDQVDAIIERAEKEGKFARKFQTKGASHTQQ
                     MDPLLGELAAELQGIEPHPLTTGYFSTVHEGGYIRPGGEPIHDVDYWKKGLRHSVYFT
                     HGIRNAVDNGHTTFLELAPNPVALMQVGLTTAASGLHEGQLIATLARKQDEVDSMTAA
                     MAQLFVYGHDLDFTTMFSRGGSGVGDFANIPLTRFRRKPHWLEARFGADSSLMMPGNH
                     VAMPDGRHVWEYSPKGLMDLGELVKVAAAQVLPEAKLIASEQRAVPGDGARLVTTLTR
                     HPGGASVQVHARIDESFTLVYDAIVSRDGQAAALPAAVATGAVVAEPVETAQAAQPAE
                     DAEILSDYLTQGANLGAGFAKWTPDTGETISARLGAIVGGAMGYEPEDLPWEVPLIEL
                     GLDSLMAVRIKNRVEYDFDLPPIQLTAVRDANLYAVEKLIEYAIEHRDEVEDLHEHQK
                     TQTPEEIAAEQATLMSGATPATVEAPAPDPQAEPETQPEAPETPVSDLPIPPPPTDPS
                     GPKANGAQPSAAALAAQVLTQEAVTEALGADVPPREAAERVTFATWAIVTGKSPGGIF
                     NALPEIDDETAENIAARLTERAEGTITVEDVKNSPTIEALATIVREHLEAGELEGFVR
                     TLRAPSKDAAGPSKIPVFVFHPAGGSTVVYEPLLNRLPADTPVYGIERVEGSVEERAA
                     EYVPKLLQMHDGPFILAGWSLGGVLAYACAIGLKQAGADVRFVGLIDAVRAGEEVPQT
                     KDEIRARWDRYARFAERTFNVTVPAIPYEQLEELDDEGQVTFVLDAVKASGVAIPGGV
                     IEHQRTSYLDNRVIDTAEIRPYDGHVTLYMADRYHDDAIFFEPRYATRKPDGGWGDYV
                     ADLEVVPIGGEHIQAIDEPYIAKVGAHMSEAINRIEAEEMQAK"
     gene            4959..>5195
                     /locus_tag="VGP27_19550"
     CDS             4959..>5195
                     /locus_tag="VGP27_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxyltransferase"
                     /protein_id="HEV7853528.1"
                     /translation="MTNKTTAALLADLREKLEQAKEPGGEKAIAKRDKKGIPSARARI
                     HALLDPGSFLEVGALCKTPGDPDALFGDGVVTGHG"
BASE COUNT          932 a         1720 c         1775 g          768 t
ORIGIN      
        1 cgttttccgg gggatatgaa cacccccgag gagatgtggc aggcgctgct ggcgggccgc
       61 gacgcgatca ccgacctgcc cgatgggcgg tgggtggagt tcctcgagga gccgactctc
      121 gcggcacggg ttcacaaggc ccgcacacgt ggcggatacc tctccgacat caagggtttc
      181 gatgccgagt tcttcgcgtt gtcgaagatg gaggcggaca acatcgaccc acagcagcgg
      241 atggcgcttg agctgacgtg ggaagccttg gaatatgccc gcatccccgc ctcgtcgcta
      301 cgtggcacga gcgtcggggt ctacatcggc aactccacca acgactacag cttcctggcg
      361 atggccgacc cgtcgaccgc ccacccgtac gccatcaccg gcacggcgag ttcgatcatc
      421 gccaaccggg tgtcgtactt ctacgacttc cgcggcccat cgatggcggt tgacaccgca
      481 tgctcgagct cactggtcgc cgtgcaccag ggtgtacagg cgctgcgcag cggcgaggcg
      541 gacgtggtgc tggccggtgg ggtcaacgcg ctggtcaccc ccctcgtcac gatcgggttc
      601 gacgaaatcg gcggcgtgct ggcgtccgac ggccggatca agtccttctc ccaggacgcc
      661 gacgggtatg cgcgctccga gggtggcggc atgctggtgc tcaagcgcgt cgccgatgct
      721 cgccgcgacg gcgacgagat catggcggtc atcgcgggca gcgctatcaa ccatgacggc
      781 cgctccaacg gcatgttggc gcccaacccc gacgcgcagg ccgaagtgct gcgcaaggcg
      841 tacaaggacg cgggcatcga tccgcgcacc gtcgactaca tcgaggcgca cggcaccggc
      901 accatcctcg gtgacccgat cgaggccgac gcgctcggca gagtcgtcgg taggggacgc
      961 gcatcggaca agccggccct gctcggcgcg gtgaaatcca atgtgggaca cctggagtcg
     1021 gcagcgggcg ccgccagcct ggccaagatg acgctggcgc tgcgcaacga caaggtgccg
     1081 ccgtccatca actacacggg ccccaacccg tatatcgact tcgacgccat acacctgaag
     1141 gtcgccgaca cggtcaccga ctggccgcgc tacagcgggc acgccgtcac cggcgtctcg
     1201 ggcttcggct tcggcggcgc gaacgcgcac ctggtgctgc gcgaggtgct gccctcggat
     1261 ctcgtcgagc ctgaacccga aaccgaagac cactccgagg gaatgcacgc cgctccgcgg
     1321 ggcgatgcca cctatgtcgg cggcgttcgg atggacgagt acggcgagtt catcgatgac
     1381 gacgaaaacg aaggcgccga attcttcggc cagcatgcct acgacaccga cgagcgcgac
     1441 ccaggtctgc ccggactgac tgatgaggcc agacgcctgc tcgaggtcgc ccgcgaggag
     1501 gccgaagccg aagagcctca cacacctgtt gtgccgctgg ctgtttcggc gttcatgacg
     1561 tcacgcaaga aggtcgcggc cgccgacttg gccgactgga tggacagcga agaaggtcgc
     1621 ggctcctcgc tggagtcgat cggcagatcg ctgtcacggc gcaaccatgg ccggtcgcgc
     1681 gccgtggtga tggcgcacga ccacgacgag gcgatcaagg ccctgcgcgc gctggccgac
     1741 ggcaagcaga gcccgaacgt gtacagcgcg gacgggcctg tcaccaacgg gccggtgtgg
     1801 gtgcttgccg ggttcggagc ccagcaccgc aagatggcca agagcctgta tctgcgcgac
     1861 gaggttttcg cggagtggat caacacggtc gacgcgttgg tccaggacga gctcgggtat
     1921 tcggtcgtcg agctgattct ggacgactcg caggattacg gcatcgaaac cactcaggtc
     1981 acgatcttcg cgatccagat cgcgctcggc gagctgctca agcaccacgg ggcgaaaccc
     2041 ggtgcagtgg ttggtcagtc gctcggtgag gctgctgccg cctacttctc tggcggtctc
     2101 tcgctcgccg acgccacccg taccatctgc tcgcgctcgc acctgatggg cgagggcgag
     2161 gcgatgctgt tcggcgagta catccggttg atggcgctcg tcgagtactc cgcagaagag
     2221 atcaagacgg tgttctccga ctatcccgat ctcgaggtgt gcgtgtatgc cgcgccgacg
     2281 cagacggtga tcggcggacc gccggatcag gtcgacgcga tcatcgagcg ggccgagaaa
     2341 gagggcaagt tcgcccgcaa gttccagacc aagggcgcca gccacaccca gcagatggac
     2401 ccgctgctcg gcgaactggc cgccgagctg cagggcatcg aaccgcatcc gctcaccacg
     2461 ggctacttct ccaccgttca cgaaggcggc tacatccggc cgggcggtga gccgatccac
     2521 gacgtggact actggaagaa gggcctgcgg cacagcgtgt acttcacgca cggcatccgc
     2581 aacgccgtcg acaacgggca caccacgttc ctcgagcttg cgccgaatcc cgttgcgctc
     2641 atgcaggtcg gcctgacgac cgcggcctct ggcctgcacg aagggcagct gatcgcgacg
     2701 ttggcccgca agcaggacga ggtcgactcg atgaccgcgg cgatggccca gctgttcgtg
     2761 tacggccacg acctcgactt caccacgatg ttttctcgtg gcggttccgg agttggcgat
     2821 ttcgccaaca tcccgctgac ccggttccgt cgcaagccgc actggctgga agccaggttc
     2881 ggcgcggaca gctcgttgat gatgccgggc aaccacgtcg ccatgcccga cggccgccac
     2941 gtgtgggagt actcaccgaa gggcttgatg gacctcggcg agctggtgaa agtggcggcg
     3001 gcgcaggtgc ttccggaagc caagctcatc gcatccgaac agcgtgcggt gcccggcgac
     3061 ggcgcccgcc ttgtgacgac gctgacacgc catcccggtg gcgcgagcgt gcaggtgcat
     3121 gcccgcatcg acgagtcgtt cacgttggtc tacgacgcca tcgtcagccg cgacggccag
     3181 gcggccgccc tgcccgctgc agtggcgacc ggagccgtcg ttgcggagcc cgtcgaaaca
     3241 gcgcaagccg cgcagccggc cgaggatgct gagatcctct ccgactacct cacacagggc
     3301 gccaatcttg gtgcgggctt tgcgaagtgg acaccggaca cgggtgagac catcagtgcg
     3361 cgtctcggcg cgatcgtcgg cggcgcgatg ggctatgagc cggaggatct gccgtgggag
     3421 gtgccgctga tcgagctggg tctggactcg ctgatggcgg tgcggatcaa gaaccgtgtc
     3481 gagtacgact tcgacctgcc gccgatccag ctgaccgcgg tgcgtgacgc caacctgtat
     3541 gccgtcgaga agttgatcga gtatgcgatc gaacatcgcg acgaggtcga ggacctgcac
     3601 gagcaccaga agacgcagac gccggaggag atcgccgccg agcaggccac gctgatgagc
     3661 ggcgccacgc cggcgacggt ggaggcaccg gcgcccgatc cgcaggccga acccgaaacg
     3721 caaccggagg caccggagac cccggtgtcg gatctgccga ttccgccgcc gccgaccgac
     3781 ccgtcgggcc cgaaggccaa cggcgcgcag ccgagcgcgg ccgcgctcgc agcccaggtg
     3841 ctgacgcagg aggccgtcac cgaggcgctg ggcgccgacg tgccaccgcg ggaagccgcg
     3901 gagcgggtca cgttcgcgac gtgggcgatc gtcaccggca agtcaccggg cggcatcttc
     3961 aacgcgttgc ccgagatcga cgacgagacc gccgagaaca tcgctgcgcg gctcaccgag
     4021 cgcgccgaag gcaccatcac cgtcgaggac gtgaagaact ctccgacgat cgaggcgctg
     4081 gcgaccatcg tgcgcgagca cctggaggcg ggcgagctcg aggggttcgt gcggacgttg
     4141 cgggcgccat cgaaggacgc agcggggccg tcgaaaatcc ccgtcttcgt gttccacccg
     4201 gccggtggtt cgacggtggt ctacgagccg ctgctgaacc ggctgcccgc ggacaccccg
     4261 gtgtacggca tcgagcgggt cgagggctcc gtcgaggagc gcgccgccga atacgttccg
     4321 aagctgctgc agatgcacga cgggccgttc atcctggccg gctggtccct gggcggtgtg
     4381 cttgcttatg cctgcgcgat cgggctcaaa caggcgggcg ccgacgtccg cttcgtcggg
     4441 ctgatcgacg cggtgcgtgc gggcgaagag gtgccgcaga ccaaggacga gatccgggcc
     4501 cgctgggacc gctacgcgcg attcgccgag cgcaccttca acgtgactgt gcccgccatc
     4561 ccgtacgagc agctcgagga gctcgacgac gaaggacagg tgacgttcgt cctggacgcc
     4621 gtcaaggcga gcggggtggc gatccccggc ggcgtcatcg agcaccagcg cacgtcgtat
     4681 ctggacaacc gggtgatcga caccgcggag atccggccgt acgacggtca cgtcacgctg
     4741 tacatggccg accgctacca cgacgacgcg atcttcttcg agccgcgcta cgccacccgc
     4801 aaacccgacg gcggctgggg cgactacgtg gccgacctgg aggtcgttcc gatcgggggc
     4861 gagcacatcc aggcgatcga cgagccgtac attgccaagg tcggcgccca catgagcgag
     4921 gccatcaatc gcatcgaagc tgaggagatg caggcgaagt gaccaacaaa accactgcgg
     4981 cactgctggc cgacctccgc gaaaagctgg agcaggcaaa ggaacccggt ggtgagaagg
     5041 ccatcgccaa gcgcgacaag aagggcattc cgagcgccag ggcccgaatc catgcgctgc
     5101 tcgaccccgg cagcttcctg gaggtcggtg cgctgtgcaa gacgccaggg gaccccgacg
     5161 cgctcttcgg tgacggggtg gtcaccggcc acggc
//
LOCUS       DASYLX010000835         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2352204,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000835 DASYLX010000000
VERSION     DASYLX010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352204"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VGP27_19555"
     CDS             <1..177
                     /locus_tag="VGP27_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV7853529.1"
                     /translation="NVVHATVAALKLLQRPEEVAARRGLPIEDVAPARMLRARREADA
                     LAATANAAAREGSA"
     gene            180..368
                     /gene="rpmD"
                     /locus_tag="VGP27_19560"
     CDS             180..368
                     /gene="rpmD"
                     /locus_tag="VGP27_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV7853530.1"
                     /translation="MAELKITQVRSTIGARWKQRESLRSLGLRKIRQSVVREDNAQTR
                     GLINTVHHLIKVEAVEGD"
     gene            372..815
                     /gene="rplO"
                     /locus_tag="VGP27_19565"
     CDS             372..815
                     /gene="rplO"
                     /locus_tag="VGP27_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV7853531.1"
                     /translation="MTIKLHDLRPAPGSKTAKTRVGRGEGSKGKTAGRGTKGTKARKN
                     VPATFEGGQMPIHMRLPKLKGFRNRFRTSYEVVNVGDLNKLFPKGGDVGVDELVAAGA
                     VRKNSLVKVLGDGKLTVKVNVTAHKFSGSAKEKISAAGGSATEAS"
     gene            complement(875..1801)
                     /locus_tag="VGP27_19570"
     CDS             complement(875..1801)
                     /locus_tag="VGP27_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7853532.1"
                     /translation="MRFSISIPQLDYYGFDDAGVKEYLARAEELGFEGGWTMEQVVGD
                     SPQIAPLAMLAYAAACTTRLRLGVAVLITSLHDPLQLAWAIAAVDRLSHGRLDVGVGH
                     GGKRRPFAAFGVQKETFVSYFTEGIELMKAAWSDEPTVTFHGRFRDVEGLPIQPKPVQ
                     RPHPPLWIGGTAPKALARGVRIADHFLGAGSSTTATFAEAVKIVRRELDRQGKDATRF
                     TIAKRVYLTVDDDARRARKRVVAGLHRIYGDMPGIEDVPVSGTPDDVVRGVREVIDAG
                     AEMVLLNPVGVAVAEDREQMERLAAEVIPQLQ"
     gene            1846..2196
                     /locus_tag="VGP27_19575"
     CDS             1846..2196
                     /locus_tag="VGP27_19575"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853533.1"
                     /translation="MAGIVERYLDAIVSHDWDVVGECVADDIIRVGPYGDRFDGKGDY
                     LAYIADTMPKLEGYSMQLHRVTYVDDGVAFAELSETVAVGGNPMRTPEVLVFEFDGER
                     RISRVDIFIQTLPR"
     gene            2328..>2743
                     /locus_tag="VGP27_19580"
     CDS             2328..>2743
                     /locus_tag="VGP27_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEV7853534.1"
                     /translation="MFSFLPGIPGADDVRGLARKVDTARHHGVPHGCVLELDLLTAPP
                     ETTGFNPMAIISGNRPLVLREAVAAIHRAAEDKRVAGLIARVQIPAAAPGPVQELRDA
                     IAAFSDVKPSLAWAETYPGTLSYYLASAFREIWMQPS"
BASE COUNT          483 a          897 c          909 g          454 t
ORIGIN      
        1 aacgtggtgc acgccaccgt tgccgcgctg aagctgctgc agcgtcccga agaggtcgcg
       61 gcccgccgtg gcctgcccat cgaggacgtg gcgcctgcgc gcatgctcag ggcccgccgt
      121 gaggccgacg cgttggccgc aaccgccaac gccgccgcac gcgaaggatc ggcatagcca
      181 tggcagagct gaagatcact caggtccgca gcacgatcgg tgcccggtgg aagcagaggg
      241 aaagcctgcg ttcgttggga ctgcgcaaga ttcgtcagtc ggtcgtccgt gaggacaatg
      301 cgcagacccg cgggctcatc aacacggtgc accacctcat caaagtagaa gcggttgaag
      361 gcgactaaat catgacgatc aaactgcacg acctgcggcc ggcgccgggg tcgaagaccg
      421 ccaagacccg cgtgggccgc ggcgagggct ccaagggcaa gaccgccggt cgcggtacga
      481 agggtacgaa ggcccgcaag aacgtccccg cgacgttcga gggtggccag atgcccattc
      541 acatgcggct gccgaagctc aagggcttcc gcaaccggtt ccggacctcc tacgaggttg
      601 tcaatgtggg cgacctgaac aagctgttcc ccaagggcgg cgacgtcggt gtcgacgaac
      661 tggtggccgc cggtgcggtc cggaagaact cgctggtcaa ggtgctcggc gacggcaagc
      721 tgaccgtgaa ggtcaacgtc accgcgcaca agttcagtgg cagcgcgaag gaaaagatct
      781 cggccgcagg cggttcggcg accgaggcct cctaactccc gcgacaagac gcagaagccc
      841 ccgacacgtt agccgtgcgg gggctttcgc gatttcactg cagttgcgga atcacctcgg
      901 cggcaagccg ttccatctgc tcgcggtctt cggcaacggc aacgccgacc ggattcagca
      961 gcaccatctc cgcgcccgcg tcgatcacct cgcgaacccc gcgcacgacg tcgtccggcg
     1021 tacccgagac gggaacatcc tcgatccccg gcatgtcccc gtagatgcgg tgcagtccgg
     1081 cgacgacgcg cttgcgggcc cgtcgagcgt cgtcatcgac ggtcagatag acgcgcttgg
     1141 cgatggtgaa tcgcgttgcg tctttgcctt gtctatcgag ttcgcgacgg acgatcttga
     1201 cggcttcggc gaaggtcgcg gtcgtcgacg agcctgctcc cagaaagtgg tcggcgatcc
     1261 gtacgccgcg cgcaagagcc ttcggcgcgg tgccgccgat ccacagcgga gggtgtggac
     1321 gctgtaccgg cttgggttgg atcggcaggc cctcgacgtc gcggaatcgg ccgtggaacg
     1381 tcaccgtcgg ctcgtctgac catgccgcct tcatcagctc gatgccctcg gtgaaatagc
     1441 tgacgaaggt ctccttctgc acgccgaagg ccgcgaacgg cctgcgtttg ccgccatggc
     1501 cgacgccgac atccagccgg ccgtggctca ggcggtcgac cgcggcgatg gcccacgcca
     1561 gctgcagcgg gtcatgcagc gacgtgatga gcaccgccac cccgaggcgc aaccgggtcg
     1621 tacacgccgc ggcgtaggcg agcatggcca gtggtgcgat ctgcggcgag tcgccgacga
     1681 cctgctccat cgtccagccg ccttcgaagc cgagttcttc ggcgcgcgcg agatactcct
     1741 tcactccggc gtcgtcgaag ccgtagtagt ccaactgggg gatcgatatc gagaaccgca
     1801 cacaaccacg ttaggcctcg agtgcagacg tggtgtgatt ggccgatggc aggcatcgtg
     1861 gagcgctatc tcgatgcaat cgtctcgcac gattgggatg tagtgggcga gtgcgtcgcc
     1921 gacgacatca tccgcgtcgg cccgtacggc gatcggttcg acgggaaggg cgactacctc
     1981 gcctacatcg ccgacacgat gccgaagctc gagggctact cgatgcagct tcaccgggtg
     2041 acctacgtcg acgacggggt ggccttcgcc gaactcagcg agacggtcgc tgtcggaggc
     2101 aacccgatgc gcacgccgga ggtgctcgtt ttcgaattcg acggtgagcg gcgtatttcc
     2161 cgggtcgaca tcttcatcca gactctgccc cggtaacgct cagcgcgctg ggccgggcgg
     2221 tgtcaatgca gaacggcgtc gcccaggaat tatcgccgcc gtcaattacc cgttggtcac
     2281 ggcgagcgaa tcgtctggtg aggcgcgctg ctcggtaggc tcgaattatg ttcagtttct
     2341 tgcccggcat ccccggcgcc gacgatgtgc gcggtctagc ccgcaaagtg gataccgccc
     2401 gccatcacgg tgtgccccac ggctgtgtgc tcgagctcga tctactgacg gcgccgcccg
     2461 agaccacggg cttcaacccg atggcgatca tcagcggcaa caggccgctg gtgctacgcg
     2521 aggccgttgc ggcaattcac cgggccgccg aggacaagcg cgtcgcaggc ctgatcgcgc
     2581 gggtgcagat tcctgccgcg gcgcccgggc ccgttcagga actgcgggac gcgatcgccg
     2641 ccttcagtga cgtcaagccg tcgctggcgt gggccgagac ctatcccggc acgctgtcgt
     2701 actacctggc ttcggcattc cgtgagattt ggatgcagcc gtc
//
LOCUS       DASYLX010000836         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2789601,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000836 DASYLX010000000
VERSION     DASYLX010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789601"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VGP27_19585"
     CDS             <1..913
                     /locus_tag="VGP27_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7853535.1"
                     /translation="AWINLDDDAPPLTDCIEPFASKHDEWKVSALRVEWWKSCLLPVN
                     WKLATAAFMEGYHVPQTHPQLLPSSHKPDASTVHPLIETSLYFMRTLGSGMGGMTHEN
                     DVRIAEGLQNMSLPDDPAEAMATWRGAVNDAVTTWHRARGCDFADLNDLERRGVLEPI
                     GFCFPHYFLLPQYSSASSYRIRPLGPEQTLFEVWSLTRYPNDRSQGRPTAPEPLAPDD
                     PSWPMIPAQDFSNLPRQQKGLHAKGFEYMRLSDRIEGLISNYERVIDGFLTGLPYERL
                     VPAIQRTNTTIDVPVADLGLTETVPAS"
     gene            910..2568
                     /locus_tag="VGP27_19590"
     CDS             910..2568
                     /locus_tag="VGP27_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV7853536.1"
                     /translation="MNWDKTVDLLIAGSGGGGMVAALAAVDAGIEPLVVEKQDLVGGS
                     TGMSGGMVWLPNNPLMRAQGIPDSHEDGLAYFDDVIGDIGPASSAARRERFLTAGFEM
                     LDFLLRKGVRMVRCPGWSDYYPNHKGGNSAGRSVEGIPFDASVLGDWRDRVQPSMARN
                     YGFVVKTNELRAVQYFNRSPRAFAVAMRVFLRTNMAKLRRRDLMTNGASMIGQILKVL
                     MDSVGDPPIWTNAAMQDLIVEDGRVVGARVSHNGSPVNIQARRGVLLAAGGFSRNADM
                     RRRYSGDQPNDGSWSIANAGDTGEVLQTAMDLGAQTDLLDEAWWLPMVFIQDPGAASL
                     GSGRQRPGAIYVDGAGKRFCNESNSYVEVGKAMYANKAVPCWQVFDEGYVGRYVSGAN
                     PLKKRTLSEGLIEQGAVKRADTITDLARQIDVPPDALEETVKRFNGFAANGLDPDFGR
                     GQSAYNDCLGDPGYKPNAALGPLDTAPYYATRVFPADVGTCGGLITNEHAQVLDERDE
                     VIEGLYATGNITATVMGRTYLGAGGSIANTMVFGYVAALHAVGR"
     gene            complement(2548..3417)
                     /locus_tag="VGP27_19595"
     CDS             complement(2548..3417)
                     /locus_tag="VGP27_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7853537.1"
                     /translation="MERRELESVIYEREPPIARIILNRPDKANTKDASLVQEVDACLH
                     EADRDKEIKVVVLKANGKGFCGGHVARWGPDENPYPDFGDTFEDLYKGTADLFLWPTL
                     YLWEFPKPTISQIHGYCMGGGIYLGLLTDFSVASDDAYFQMPLAQSLGEPGGHTMIEP
                     WLMMNWHRTMDWLLLAPTLSAQEALEWGLLNKVVPREQLEDTVEEMARKISQIPLTTL
                     MAVKNNVKRAWELMGMRVHLQMSHVMTNMVGAASDVQARRTELIQSGLKPRDFVADGE
                     TSPGEGKSAADGV"
     gene            complement(3429..4649)
                     /locus_tag="VGP27_19600"
     CDS             complement(3429..4649)
                     /locus_tag="VGP27_19600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7853538.1"
                     /translation="MTQVLTGVRVIELASWTYVPSAGAALADWGADVIKVEDVKAGDP
                     GRALVIGGFTRQNARADVDFILEIGNRGKRSVAIDIKSDTGREYFGRLLASADVFLTN
                     WLPAPLERARLTIEEIRSFNPNIIIARGTGTGVRGPDRNKGGFDAATYLSRGGVSYTM
                     TPFGTETPAVQGPGFGDLQGGITLAGGVCAALFRRERTGEPSIVDSSLLAQAMWTIAP
                     SISVADLFGIDGIPGAPPGLAINPLVNRYKTRDGRWLQMVFLQPDRFWAGFCERIGLP
                     ELASDERFVPSSNLIANAEEACKLVGERLASEDLEYWRAALADEPGVWAALATPKETL
                     NDPQVIPNGYVIPNVDDNGVEYQVVAAPVQFDETPPTAARAPEHGQHTEEILLELDLD
                     WDDISAAKDSGAIL"
     gene            complement(4646..>5651)
                     /locus_tag="VGP27_19605"
     CDS             complement(4646..>5651)
                     /locus_tag="VGP27_19605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853539.1"
                     /translation="HVRPLLDDPTGTTDVVWRGLADLGATGVLVPSDHDGAGMTMVEA
                     GIVAEELGAALNPGPWLSSAVAATRALTRTGAGSDLLSEIACGTEVVTVGPLTGARPA
                     ANGEEVRGVLTAAPDVAAATVLLVLADEPAGTALYAVDPTSSDVTVTARPHVDPTRKL
                     FDVVLDDVQSRRLAEASAGAIEGLVDDVLIANAADALGAATAVMNLAVEYAKVRRQFD
                     QPIGSFQAVQHLCVDMYEAVELARSGVIHALWAADAAEPDERRRAALRAKAFAGRLTS
                     VGDTAIQVFGGIGFTWEHDAHLYLKRLLGWSAFLGAPDRYLEEVGAYLVSDLDRKVLP
                     "
BASE COUNT          941 a         1892 c         1835 g          983 t
ORIGIN      
        1 cgcgtggatc aacctcgacg acgacgcacc gccactgact gactgcatcg aaccgttcgc
       61 ctccaagcac gacgagtgga aggtctcggc gctgcgcgtc gagtggtgga aatcgtgcct
      121 gctgccggtg aactggaagc tcgcgacggc ggcgttcatg gagggctacc acgtcccgca
      181 gacccatccc caactgctgc cctcctcgca taagcccgac gcctcgacgg tgcatccgct
      241 catcgagacc agcctctact tcatgcgcac cttgggctcc ggcatgggcg ggatgacgca
      301 cgagaacgac gtgcggatcg ccgaggggct gcagaatatg tcgcttcccg acgatccagc
      361 cgaggcgatg gccacctggc gcggcgcggt caacgacgcc gtcacgacct ggcatcgcgc
      421 ccgcggctgc gacttcgccg acctgaacga cctcgagcgc cgcggtgttc tcgagcccat
      481 cgggttctgc ttcccgcact actttctttt gccgcagtac agcagcgcgt cgtcctaccg
      541 gatccgcccg ctcggacccg agcagacgct gttcgaggtc tggtcgctga cccggtaccc
      601 gaacgaccgc tcgcagggaa ggcccaccgc gccagagcca ctcgcaccgg acgatccgag
      661 ctggccgatg attcccgcac aggacttctc aaacctcccg agacagcaga aaggcctgca
      721 tgccaaaggg tttgagtaca tgcggttgtc ggatcggatc gaaggtctga tctcgaacta
      781 cgagcgggtc atcgacggct tcctcaccgg cttgccgtac gagcggctgg tgcctgcgat
      841 ccaacgcacc aacaccacca tcgacgtacc cgtcgccgat ctcgggctga ctgaaacagt
      901 gcccgcctca tgaattggga caaaactgtc gaccttctga tcgcaggcag cggaggcggt
      961 ggaatggtcg ccgcgctggc cgcggtcgat gcgggaatcg agccgctggt cgtcgaaaag
     1021 caggatctcg tcggcggttc caccggaatg tccggcggca tggtctggtt gccgaataac
     1081 ccgctgatgc gggcccaggg aataccggat tcgcacgagg acgggctggc gtacttcgac
     1141 gacgtcatcg gcgacatcgg tcctgcgtcg tcggctgccc gccgcgagag gtttctcacc
     1201 gccggcttcg agatgctcga tttcctgctg cgcaagggag tccggatggt tcggtgtccc
     1261 ggctggagcg actactaccc gaaccacaag ggcggcaatt cggcgggccg ctccgtcgag
     1321 ggcatcccgt tcgatgcctc cgtgctcggc gactggcgtg acagggtcca gccatcgatg
     1381 gccaggaact acgggttcgt cgtcaagacc aacgaactgc gtgctgtgca gtacttcaac
     1441 cggtcgccgc gcgcctttgc cgtcgcgatg cgggttttcc tgcgcaccaa catggccaag
     1501 ctgcggcgtc gggacctgat gaccaacggc gcgtcgatga tcggtcagat cctgaaggtg
     1561 ctgatggatt cggtgggcga tccgccgata tggacgaatg cggcgatgca ggacctcatc
     1621 gtcgaggacg gccgtgtcgt cggcgcgcgt gtcagtcata acggctcgcc ggtgaatatc
     1681 caggcccgca gaggagttct gctggcggcc ggcgggttca gccgcaacgc cgacatgcgt
     1741 cgccgttaca gcggcgacca gcccaacgac ggatcgtggt cgatcgccaa cgcgggcgac
     1801 accggcgagg tcctgcagac ggcgatggac ctgggtgcac agaccgatct gttggacgaa
     1861 gcttggtggc tgccgatggt tttcatccag gatcccggtg ccgcatcgct gggttccgga
     1921 cggcaacgcc ccggcgccat ctacgtcgac ggggcgggca agcggttctg caacgagtcg
     1981 aactcctatg tcgaagtcgg caaggcgatg tatgccaaca aggccgtgcc gtgctggcag
     2041 gtcttcgacg agggttacgt cggccggtat gtgtccggtg ccaacccgct gaagaagcgc
     2101 acgctgtccg aggggctgat cgagcagggt gccgtcaagc gagccgacac gatcaccgac
     2161 ctggcccgcc agatcgatgt gccccccgat gcgctcgagg aaacggtcaa gcgcttcaac
     2221 gggtttgcgg ccaacggcct cgaccccgac ttcgggcgcg gccagtcggc gtacaacgac
     2281 tgcctcggcg accccggata caagcccaac gccgcgctgg gtccgctcga cacggcaccg
     2341 tattacgcca cccgagtgtt ccctgccgac gtcggaacgt gcggcggcct gatcaccaac
     2401 gagcacgcac aggtcctcga cgagcgtgac gaagtgatcg aggggctgta cgcgaccggc
     2461 aacatcaccg cgacggtgat gggccgcacc tatctgggtg ccggcggcag catcgccaac
     2521 acgatggtgt tcggctacgt cgcggcgcta cacgccgtcg gccgctgatt tcccctcgcc
     2581 gggagaagtc tccccgtcgg cgacgaaatc ccttggcttc aagcccgact ggatgagctc
     2641 cgttcgacgt gcctgcacgt cggacgctgc gccgaccatg ttcgtcatga cgtggctcat
     2701 ctgcagatgc acgcgcatac ccatcaattc ccatgcccgc ttcacgttgt tcttcactgc
     2761 catcaacgtg gtcaacggga tctgcgagat cttgcgggcc atctcctcga cggtgtcctc
     2821 gagctgctcg cgcggcacca ccttgttgag caacccccac tcgagcgcct cctgcgcgga
     2881 cagcgtcggc gccagcaaca gccagtccat cgtgcggtgc cagttcatca tcagccacgg
     2941 ctcgatcatc gtgtgcccgc cgggttcgcc gaggctctgc gcgagcggca tctggaagta
     3001 cgcatcgtcg gaggcgacgg agaagtcggt caacagcccg agatagatcc cgccgcccat
     3061 gcagtagccg tgaatctggg agatcgtcgg cttgggaaac tcccacaggt acagcgtcgg
     3121 ccatagaaag aggtcggcgg tgcccttgta cagatcctcg aacgtatcgc cgaaatccgg
     3181 ataggggttc tcgtcgggcc cccacctggc aacgtggccg ccgcagaacc cttttccgtt
     3241 ggctttcagg accaccacct tgatctcttt gtcgcggtcg gcctcgtgga ggcacgcgtc
     3301 gacttcctgc acgaggctgg cgtccttcgt gttcgccttg tcgggccggt tcaggatgat
     3361 ccgggcgatc ggcggttcac gttcgtagat caccgattcg agctcgcgcc gctccatcat
     3421 tggcctccct agagaatcgc gccgctgtcc ttggccgccg agatgtcgtc ccagtcgaga
     3481 tcgagctcca acaggatctc ctcggtgtgc tgcccatgtt cgggagcccg cgcggcggtc
     3541 ggcggcgttt cgtcgaattg cactggtgcg gcgacgacct ggtactcgac accgttgtcg
     3601 tcgacgttgg ggatgacgta gccgttcggt atcacctgcg gatcgttgag ggtctccttc
     3661 ggcgtggcca gcgccgccca gacccccggc tcgtcggcga gtgctgcacg ccagtactcg
     3721 agatcctcgc tcgcgagcct ctctccgacg agcttgcacg cctcctcggc gttggcgatc
     3781 aagttgcttg acgggacgaa gcgctcgtcg gacgcaagct cgggcagtcc gatccgctcg
     3841 cagaagcctg cccagaatcg gtccggctgc aaaaagacca tctgcagcca cctgccgtcg
     3901 cgcgtcttgt agcggttcac caaagggttg atcgccagtc ccggcggtgc accggggatt
     3961 ccgtcgatgc cgaataggtc tgcgaccgag atcgacggcg cgatggtcca catcgcctgt
     4021 gcgagcagcg acgagtcgac gatggacggt tcgccggtgc gctcccggcg gaaaagcgcc
     4081 gcgcacacgc cacccgcgag ggtgatgccg ccctgcagat cgccgaaccc cggcccctgc
     4141 actgccggtg tctcggtgcc gaacggggtc atggtgtagg aaaccccgcc gcgcgacagg
     4201 taggtggccg cgtcgaaccc gcccttgttc cggtcgggtc cccgcactcc ggtgcccgtg
     4261 ccgcgggcga tgatgatgtt cgggttgaac gagcggattt cctcgatggt cagccgcgcc
     4321 cgttccagcg gggcgggcag ccaattggtc aggaacacgt ctgcgctggc gagcaggcgg
     4381 ccgaaatact cacggccggt gtcggatttg atgtcgatcg cgacgctgcg tttgccgcgg
     4441 ttgccgatct cgagaatgaa gtcgacgtcg gccctggcgt tctgccgggt gaagccgccg
     4501 atgaccagcg cccggcccgg gtcgcccgcc ttcacgtcct ccaccttgat gacgtcggcg
     4561 ccccaatccg ccagcgcggc gcctgcagat ggcacgtatg tccaggaggc tagctcgatg
     4621 acccgcactc cagtgaggac ttgtgtcatg gcaacacctt tcggtcgagg tcgctgacga
     4681 ggtacgcgcc gacctcctcg aggtatcggt cgggagcgcc gaggaacgcg ctccagccga
     4741 gcaggcgctt gagatagagg tgggcgtcgt gctcccaggt gaagccgatg ccgccgaaca
     4801 cctggatcgc ggtgtcgccc acggaggtga gtcggcccgc gaacgccttg gcgcgcagtg
     4861 cggcacggcg gcgttcgtcg ggctcggcgg cgtcggcggc ccacaatgcg tgaatgactc
     4921 cgctgcgggc gagttcgacc gcctcgtaca tgtcgacgca caggtgctgg acggcctgga
     4981 agctgccgat cggctgatcg aactgcctgc gcaccttcgc gtattcgacg gcgaggttca
     5041 tcaccgcggt ggcggcgccg agcgcgtccg cggcgttcgc gatcagtaca tcgtcgacga
     5101 gtccctcgat cgcgcctgcc gaagcttcgg ccagccgccg cgactgcaca tcgtcgagga
     5161 cgacgtcgaa gagcttgcgc gtcgggtcaa catgcggccg ggctgtcacc gttacgtcgc
     5221 tggaagtcgg gtcaacggcg tacagcgcgg tgccggccgg ctcgtcggcg agaaccagca
     5281 gcacggtcgc ggccgccacg tctggcgctg cggtcagcac tccgcgcacc tcctcgccat
     5341 tggcggccgg ccgcgcaccg gtcaacggcc caaccgtaac tacctctgtc ccgcacgcga
     5401 tttcactcaa caggtcactg cccgcgccgg ttcgtgtcag agcacgtgtc gcggcgaccg
     5461 cactcgacag ccaggggccg gggttgagcg cggcgcccag ctcttcggcg acgatgccgg
     5521 cctcgaccat tgtcatgccc gcaccgtcgt ggtccgacgg gacgagcacg cccgttgcgc
     5581 cgagatccgc cagaccacgc cagaccacgt cggtggtacc cgtcggatcg tccagcagtg
     5641 gccggacatg t
//
LOCUS       DASYLX010000837         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_47425, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000837 DASYLX010000000
VERSION     DASYLX010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47425"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGP27_19610"
     CDS             complement(<1..670)
                     /locus_tag="VGP27_19610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853540.1"
                     /translation="MRWGSDTRRTCHNHSVQSPINGHVSNWFDELPACRPALPGDRDA
                     DVCIVGAGYTGLWTAYYLKRADPSLRVAILEARFAGFGASGRNGGWLSGLVPGDRHRM
                     ATQHGRDGVVAWQRALNDAVDEVIDVAAREGIDAGIAKGGTLQIARNSAQAARLAAEA
                     EEELSWKVDGIAVLTRSEAAERIRFAHVVAAYHNPHCARIQPAQLARGLADTVAQLGV
                     DIYEQ"
     gene            702..1172
                     /locus_tag="VGP27_19615"
     CDS             702..1172
                     /locus_tag="VGP27_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HEV7853541.1"
                     /translation="MCWQCDHPGATTADYLVELRKTIRKCKWAVQYVEDDKKPFAYTI
                     GLHKRGFTEYLVTGVSPEEAGRLLNAVADYTIREVQPKPGDTMEIAGEVQLEFVKVEQ
                     PDAHLNLAIALYGPDVQALQAVWRDDHGHSPWCPGFDSGRGSQPVLGKRGPQKA"
     gene            complement(1160..1933)
                     /locus_tag="VGP27_19620"
     CDS             complement(1160..1933)
                     /locus_tag="VGP27_19620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853542.1"
                     /translation="MLATFYAKALDAGLDKPILGDRWAKDIVDRIDYDWSKTTITARN
                     SPSVTTRSAHFDNWARQFLAVHPEATVLHLGCGLDSRYYRLDPGPGVEWYDVDYPDVI
                     VLREQLFPPREHCHVVAASVTDPAWLSAVPADRPTLMIGEGLTMYLTEADGIALLRRV
                     VDHSPLGELQFDAFSRFGIKTQWINAVVKRAGATLHWGIDGSDDILQAVPGLRLLAWV
                     SPFDSPTFADVSLPYRVMARTMSLSSKLRYMAQYHRYAF"
BASE COUNT          388 a          717 c          671 g          344 t
ORIGIN      
        1 actgctcgta gatgtccacg cccagttggg ccacggtgtc ggcaagcccg cgggcgagct
       61 gggcgggttg gatgcgggca cagtgcggat tgtgataggc cgccaccaca tgagcgaatc
      121 gaatgcgttc ggccgcttcg gatctcgtga gcaccgcgat gccgtcgact ttccagctga
      181 gctcctcttc cgcctctgcg gccagccgtg cggcctgcgc ggagttccgg gcgatctgca
      241 acgtgccgcc cttggcgatg cccgcatcga tgccttcgcg tgccgcgacg tcgatcacct
      301 cgtcgacggc gtcgttcagc gcccgctgcc aagccaccac gccgtcacgg ccgtgctgcg
      361 tggccatccg gtggcggtcg ccggggacca ggcccgacaa ccagccaccg ttgcgccccg
      421 acgccccgaa accggcgaac cgagcctcga ggatcgctac ccgcagcgac gggtccgcac
      481 gcttgaggta gtacgccgtc cacagcccgg tgtaaccggc gccgacgatg cacacgtcgg
      541 cgtcgcgatc gcccggcagc gcgggccggc aggcgggtag ttcgtcgaac cagttggata
      601 cgtggccgtt gatcggggac tgcaccgagt gattgtgaca ggtccgtcgc gtgtcagacc
      661 cccagcgcat tgtggcggca agtactacga aaggacacgc catgtgctgg caatgcgatc
      721 accccggcgc aaccaccgcc gactacctcg tcgaattacg caagacgatc cgcaaatgta
      781 aatgggcagt gcagtacgtc gaagacgaca agaagccgtt cgcctacacg atcggtctgc
      841 acaagcgcgg cttcaccgag tacctcgtca ccggcgtatc tcccgaggag gccggccggc
      901 tactgaacgc ggttgccgac tacaccatcc gagaagtaca acccaagcct ggcgacacca
      961 tggagatagc cggggaagtg cagctggagt tcgtcaaggt cgaacagccc gacgcgcacc
     1021 tcaatctcgc gatcgctctc tacggtcccg atgtgcaagc gctacaggca gtttggcgtg
     1081 acgatcacgg gcattcgcca tggtgccccg gcttcgactc cggccgcggc agccaacccg
     1141 tgctcggaaa gcggggtcct cagaaggcgt agcggtggta ttgcgccatg tagcgcagct
     1201 tcgacgacaa cgacatcgtc ctggccatca cccggtaagg aagcgacacg tcggcgaagg
     1261 tcggcgagtc gaacggtgag acccaagcca gcagtcgcaa tccgggcacc gcctggagga
     1321 tgtcgtccga accgtcgatg ccccagtgca gcgtcgcgcc cgcccgcttc accactgcgt
     1381 tgatccattg cgtcttgatc ccgaaccggc tgaacgcatc gaactgcaac tcacccaatg
     1441 gcgaatggtc gacgacccgg cgcagcagcg cgatcccgtc cgcctcggtc aggtacatgg
     1501 tcaacccctc gccgatcatc agcgtcggcc gatcggcggg aacggcggag agccaggccg
     1561 gatccgtcac ggaggccgcg acgacatgac agtgttcgcg cggtgggaac aactgctccc
     1621 gcagcacgat cacgtcgggg tagtcgacgt cgtaccactc gacacccggc ccggggtcaa
     1681 gccggtagta gcggctgtcc aggccgcagc ccagatgcag cacagtcgcc tcgggatgca
     1741 ccgcgagaaa ttggcgtgcc cagttgtcga agtgcgccga acgagtggtg accgacggcg
     1801 agtttcgtgc agtgatcgtc gtcttggacc agtcgtagtc gatccggtcg acgatgtctt
     1861 tggcccaccg gtcgccgagg atcggcttgt ccaagccggc gtccagcgcc ttggcgtaga
     1921 acgtcgccaa catggtctgc ggcgccccgg acagatcgac gtgcagtttc tccgccacac
     1981 cccagaggct agtcccgcgg ccacctcacg ccgagaacct gaacggcagg ccgcgcgtcg
     2041 ttcagccggc ctgcttgctc ttgcgtttgg tgtttcgaag gttcacccag aacagcgcag
     2101 cctcgcggat cgactcctcc
//
LOCUS       DASYLX010000838         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_157844,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000838 DASYLX010000000
VERSION     DASYLX010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157844"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGP27_19625"
     CDS             complement(<1..365)
                     /locus_tag="VGP27_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853543.1"
                     /translation="MSDTRVHAGGGRLQMPRSRGAASGFLLILLGIWGALVPFVGPLF
                     DFAFSPDREWVWTAARGWLEVLPGAATAVGGFLLLSSRNRFTAMLGGWLTVLAGAWFV
                     VGRALAGPLALGDPGVPVAA"
     gene            527..1618
                     /locus_tag="VGP27_19630"
     CDS             527..1618
                     /locus_tag="VGP27_19630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7853544.1"
                     /translation="MGWLRQWWAQPDHFRWLSGYLGYRNLRGFTRRMMATVVIALGAA
                     PILTMWTPVGPRSTAGWVIGVLVAVCCGVMAMMWLIEWPSRRQSVQFVLLADACIAAS
                     CLVYASPGMALLGCTTFAALAGYVAFFHTSRYLAVILATAAATSTFCAVQLAMDSGVL
                     VAVAKLLIIAVGVLAVPFSAHVLVHLLGDEALKSHTDPLTGLRNRRGFYRSARELIGA
                     SVDAAEPYLTVIMIDLDRFKQVNDTRGHTTGDRILVAVADKLRHTTRGRAVVARVRGE
                     EFLIAETTQPGEAHAIAERLRREVAAIPRGATASVGVASITREGAAEVAARTVIEHLV
                     DAADTAMYEAKRAGGNQTRHAESGWIHST"
BASE COUNT          318 a          646 c          580 g          302 t
ORIGIN      
        1 gcggccaccg gtaccccagg gtccccgaga gccagcggcc cggccagcgc tcgccccacc
       61 acgaaccagg ccccggcgag cacggtcaac cagcccccga gcattgcagt gaaccggttg
      121 cgcgaactca gcagcaagaa accgccgacg gcggtggccg cgccgggcag cacctccagc
      181 cagccacgtg ccgccgtcca cacccactcc ctgtcggggc tgaacgcgaa atcgaacaac
      241 gggccgacga acgggacgag cgcgccccaa atgcccagca gaatcaacag aaagccactt
      301 gccgcgccac ggctgcgcgg catttgcaac ctgccgccac cggcatgaac acgcgtgtca
      361 ctcattgaag cctcctcgtc ccgacggctt agcccgaccc gccgcggaat accccaaccg
      421 gccggccgat aaccagttat gggccgattc gggctctgca accgctgtct cggccgctcc
      481 ggaattgctg agtcgcgctt cagcaaaggt cccttagacg gcttaagtgg gctggttgcg
      541 acagtggtgg gcgcagcccg accatttccg ctggctctcc ggctaccttg gataccggaa
      601 tctgcgcggt ttcacgcgcc gcatgatggc tacggtcgtc atcgcactcg gcgccgcgcc
      661 gatcctgacc atgtggacac cggtcgggcc acgcagcacg gccggctggg tgatcggcgt
      721 cctcgttgcg gtttgctgcg gcgtcatggc gatgatgtgg ctgatcgaat ggccgagcag
      781 gcggcagtcg gtacagttcg tactgctcgc cgatgcctgc atcgccgcgt cgtgcttggt
      841 gtacgcctcc cccggcatgg cgctgctggg ctgcaccacc tttgccgcac tcgccggcta
      901 cgtcgcgttc ttccacacca gccgctacct tgcggtcatc ctggcgactg ccgcggcgac
      961 gtccactttc tgcgccgtgc agttagcgat ggactcgggc gtactcgttg ccgtggctaa
     1021 gttgctgatc atcgccgtcg gagtgctcgc cgtgccgttc tcggcccacg ttctggtcca
     1081 cctgctcggc gatgaagcgc tgaagtcgca taccgatccg ctgaccggcc ttcgtaaccg
     1141 gcgcgggttc tatcgctcgg cccgcgaact gatcggtgcg tccgtcgacg cggcggagcc
     1201 gtacctgact gtgataatga tcgacctcga tcgcttcaag caggtcaacg acacccgggg
     1261 gcacaccacg ggcgatcgca tcctggtcgc ggtcgccgac aagctgcggc acaccacacg
     1321 cgggcgtgcc gtcgtcgccc gggtgcgcgg cgaggagttc ctgatcgcgg agacgacgca
     1381 gcccggcgag gcgcacgcga tcgccgagcg gttgcgaagg gaggtcgccg cgattccgcg
     1441 gggcgcgacc gcgagcgtcg gtgtagccag catcacacgc gaaggtgccg ccgaggtggc
     1501 tgcccgaacg gtgatcgagc acttggtcga tgccgccgat accgcgatgt acgaggcgaa
     1561 acgcgcgggc gggaaccaga cccggcacgc agagagcggc tggattcaca gcacatagac
     1621 agcctatcag tttgcgggaa taaagttagc tccgctaatg tagttccatg taggcgcacc
     1681 gtcgcgtcgc ccattcccgc tattctccga ggatttttga tgccgattga cacccgcgac
     1741 cagcgactcg aacgccgcat cgccaatctg aaaaactccg acgcgcagtt cgccgccgcc
     1801 acccccgacg acgcgatttc ggcggcgatc gaacggcctg aagccc
//
LOCUS       DASYLX010000839         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1363731,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000839 DASYLX010000000
VERSION     DASYLX010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363731"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGP27_19635"
     CDS             <1..444
                     /locus_tag="VGP27_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853545.1"
                     /translation="CTGALTNFAFVLGVITLVVGGFGLVAFISSTPSRALGGIFLAVG
                     IVFSVLTFLVARKIRSRRGRPLNQCRPVAVIDRKLGLFSYRGGALVQLDQVRFARKMQ
                     MGSSSPKLVALIPGGTLILKRGNPFGGGIGNVDEILTAVSAGSSV"
     gene            complement(646..1911)
                     /gene="hisS"
                     /locus_tag="VGP27_19640"
     CDS             complement(646..1911)
                     /gene="hisS"
                     /locus_tag="VGP27_19640"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV7853546.1"
                     /translation="MTSFQAPKGVPDYLPPDSAQFVAVRDGLLTAARRAGYADIELPI
                     FEDTALFARGVGESTDVVSKEMYTFTDRGDRSVTLRPEGTAGVIRAVIEHGLDRGALP
                     VKLCYSGPFFRYERPQAGRYRQLQQVGVEAIGVDDPMLDAEVIAVADAGFRSLGLDGF
                     RLEVTSLGDDSCRPQYRELLQDFLFKLDLDDETRRRAEINPLRVLDDKRPFVRELTAD
                     APVMLDHLSDSAKQHFDTVLAHLDAIGVPYAINPRMVRGLDYYTKTTFEFVHDGLGAQ
                     SGIGGGGRYDGLMGRLGGKDLSGIGFGLGVDRALLALKAEGKTIGDAARVDVFGVPLG
                     EQAKTRLSVLAGRLRAAGVRVDLAYGDRGMKGAMRAADRSGASIALVAGDRDIEAGTV
                     GVKDLSTGEQVDVAADAVVAEVLSRLGRR"
     gene            complement(1935..>2441)
                     /locus_tag="VGP27_19645"
     CDS             complement(1935..>2441)
                     /locus_tag="VGP27_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7853547.1"
                     /translation="HGHIDHIWSAQKVADTYGCPAYIHPDDRFMLSDPIKGFGPRLGQ
                     IAFGALFREPKQVLELDRDGDKLDIGGTSSITVTVDHTPGHTRGSVVFRVDGDGSQVA
                     FTGDTLFKQSVGRTDLPGGSGRDLLGSIVTKLMVLDDDTVVLPGHGEKSTIGFERRAN
                     PFLEGLTL"
BASE COUNT          399 a          837 c          802 g          403 t
ORIGIN      
        1 tgcacgggcg cgctcacaaa cttcgcgttc gtcctcgggg tgatcacact cgtcgtcggc
       61 ggcttcggcc tggtggcatt catttcgagc acgccgtcgc gagcattggg cggcatcttc
      121 ctcgccgtcg gaatcgtctt ctcggtactg acctttctgg tggcgcgcaa gatccgctcc
      181 cgccgcggcc ggccgttgaa ccagtgccgt ccggtggcgg tgatcgaccg caagctcggc
      241 ctgttcagct accgtggcgg cgcactggtg cagttggacc aggtgcgctt cgcgcgcaag
      301 atgcagatgg gttcgagctc accgaaactg gtggctctca tccccggcgg cacgctgatt
      361 ctcaagcgcg gcaacccgtt cggaggcggc atcggcaacg tcgacgagat cttgacagcc
      421 gtcagcgcag gttcgtcggt ttgagatcgt cggacatctg ttgaaccgcc tgcaccgcga
      481 gcgtgcgtgt ctgtgcgccg acacgccgcg ccacaggcgc actacgcgcg cgctcacccg
      541 tcgtcgagcg tgcacaaact gccccatttc accggcgcgt cggcgagcag acacgcacgc
      601 tcgcggggga aggtgaggtc agagggcagg gcgcacggcc ggctgctagc ggcgaccgag
      661 gcgcgacagc acctcggcga ccacggcgtc ggccgcgacg tcgacctgct cccctgtcga
      721 cagatccttc acgccgacgg tgcccgcctc gatgtcacgg tcacctgcca ccagcgcgat
      781 cgacgcgccc gaccggtccg cggcgcgcat cgcacccttc atcccgcggt cgccgtaggc
      841 gaggtccacc cgcacacccg ctgcccggag ccggccggcc agcaccgaca accgcgtctt
      901 ggcttgctcg ccgaggggca caccgaacac gtcgacgcgt gcggcgtcgc cgatcgtctt
      961 gccctcggcc ttcaacgcca gcagcgcccg gtccacgccg agcccgaacc cgatccccga
     1021 caggtcctta ccgccgagcc tgcccatcag cccgtcgtag cgaccaccgc cgccgatgcc
     1081 ggactgggca ccgaggccgt cgtgcacgaa ctcgaacgtg gtcttcgtgt agtagtccag
     1141 tccgcgcacc atgcgcggat tgattgcgta cggcaccccg atcgcatcga ggtgggccag
     1201 cacagtgtcg aagtgctgtt tggccgagtc ggagaggtgg tccagcatga ccggagcatc
     1261 agcggtcagc tcacgtacaa acggccgctt gtcgtcgagc acccgcagcg ggttgatctc
     1321 cgcgcgacgg cgggtctcgt cgtcgaggtc gagcttgaac aggaagtcct gcaacaactc
     1381 tcgatactgt ggccggcagc tgtcatcgcc gagcgaggtc acctccaacc ggaacccgtc
     1441 gagacccaac gaccgaaaac ctgcgtctgc gaccgcgatc acctccgcgt cgagcatcgg
     1501 gtcgtcgact ccgatggcct ccaccccgac ctgctgcagt tgccggtagc ggccggcttg
     1561 ggggcgttcg taccggaaga acggccctga gtagcagagc ttcaccggca gggccccgcg
     1621 atcgaggccg tgttcgatca ctgcgcgaat cacgcccgcg gtgccctccg gccgcagcgt
     1681 caccgaacgg tcgcctcggt cggtgaaggt gtacatctcc ttgctgacca catccgttga
     1741 ctcgcccacc ccgcgggcga acagcgcggt gtcctcgaag atgggcagct cgatgtcggc
     1801 atagccggcg cggcgcgcag ccgtcagcag gccgtcgcgc accgccacaa attgggcaga
     1861 gtccggcggc aggtagtcgg gcacgccttt gggcgcctga aaagaggtca cgcggacgtg
     1921 aggagcagaa gaagtcacag agtcagacct tcgaggaacg ggttggcgcg gcgttcgaag
     1981 ccgatggtcg acttctcgcc gtgcccaggt agtaccacgg tgtcgtcgtc gagcaccatc
     2041 agttttgtca cgatcgaccc cagcaggtcg cggccgctgc ctccgggcag gtccgtgcgg
     2101 ccgaccgact gcttgaacaa cgtgtcgccg gtgaaagcga cctgggagcc gtcaccgtcg
     2161 accctgaaaa cgaccgaacc cctggtgtgg cccggcgtgt ggtcgacggt caccgtaatg
     2221 ctgctggtgc cgccgatgtc cagcttgtcg ccgtcgcggt ccaattcgag tacctgtttg
     2281 ggctcgcgga aaagggcgcc gaacgcgatc tggccgagcc ggggaccgaa ccccttgatg
     2341 gggtcggaca gcatgaaccg gtcgtcgggg tggatgtatg cggggcatcc gtaagtgtcg
     2401 gcgaccttct gcgccgacca gatgtggtcg atgtggccgt g
//
LOCUS       DASYLX010000840        12242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3339904,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000840 DASYLX010000000
VERSION     DASYLX010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3339904"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VGP27_19650"
     CDS             <1..727
                     /locus_tag="VGP27_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7853548.1"
                     /translation="SLHRSMQELQLLSTEGESQEPAMAGVSASYNGHFVRCFDLTNEH
                     YVNALKGKNLADMSAIERMHLVPPPGMSPEMVDGLPNRFDDGQLKVLAGYPPQVGQLW
                     PNTAALCMPFQFPDGTPSAFLSWRVWVPRGPEHFELFHWSLVERDAPAELRDTVNLMT
                     AATFGISGFVEADDTDTWPMQTYASKGALGAQQKLRYQAITGENKPSDWPGPAQVYAG
                     FPKDDTQWNFWLRYSEFMEGRAW"
     gene            724..1320
                     /locus_tag="VGP27_19655"
     CDS             724..1320
                     /locus_tag="VGP27_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEV7853549.1"
                     /translation="MTTEATRDEVVPAVRATMGTVDYPSAKGARIKVTDPRHFEIVAW
                     LEDESTLLDDDNLTGWLALLAPDVIYRAPVRTTRDHRSKGVFEAEMYHFNENIMTLML
                     KLMRLTQTDSAWSENPLSRTQRIVHKVRAYETDKADEYEVFSSIVLIRSRYDEPALEF
                     LPARRVDTIRTGDEWKLAARTIYFDQATLGVQNLAVYL"
     gene            1332..1907
                     /locus_tag="VGP27_19660"
     CDS             1332..1907
                     /locus_tag="VGP27_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV7853550.1"
                     /translation="MSSATDEMQGYLPGIWSIDAVRSTLTFSVGHLGVHTVHGTLGVS
                     GQITMADDPLDSAVDASIDLGQVDTGSKGRDNAIRSAHLLNVASRPTATYRSRRIGIG
                     TMPGEFLLHGDLSLLGVTREVPLRIRLERSTDGDRHRPVVTGTGEFARRDFGFVYRVR
                     PRFLDRAISSTVGVEVRLEASPTDTTEGTGR"
     gene            1919..2746
                     /locus_tag="VGP27_19665"
     CDS             1919..2746
                     /locus_tag="VGP27_19665"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7853551.1"
                     /translation="MRHEDADVWGLVDVDTETVQPIRGGIEQWGPAAADGTADFEKVG
                     RPLAMTDVRWLPPITPTSTIVGVGMNYWSHLEKLGVTERPKSTLGFLKPQVAIIGHGD
                     TLANPAITEQLDFEVELVVVMAESVSRVGGVPTDAVLGYTVGNDVSARDAPAPMSGGL
                     DLFSIKALGGTTPVGPWITTKDEFGGSGQIDVGISLRVNGEERQHDRTGKMIFSIDEC
                     LEYVVARMALTAGDIVFTGTTHGVGFEDGRYLEEGDVVEAEVEGVGVLRNVVGARST"
     gene            complement(2751..3353)
                     /locus_tag="VGP27_19670"
     CDS             complement(2751..3353)
                     /locus_tag="VGP27_19670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853552.1"
                     /translation="MATRATTEARIFDGALHAIARQGPKKLAMSDIAHEAGVSVGTLY
                     RYFKNKDELLQSLGDHFVTKLQAVLQDAIEGNPEPADRLQIVIDVMLRFWLENPATVQ
                     IGQLEPGFVLGYIKRVTPRLSTVLRDALEPVLGDSAAVLGGSATLDEIADLIVRMAFS
                     HYFMPSRDYREFRDVLVVLGASAGLEPRKSRVRSDRKAVS"
     gene            4249..4959
                     /locus_tag="VGP27_19675"
     CDS             4249..4959
                     /locus_tag="VGP27_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853553.1"
                     /translation="MALDTLVQIPRRPFATRELVLQSWFVARVSLLPTLMLSIPFTVL
                     TVFTLNVLLLEFGAGDFSGSGAALAGVTQIGPLVSVLVVAGAGATAMCADLGSRTIRE
                     ELDALRVLGINPVQALVVPRVLAATLVSMLLSGLVIVTGLVGGFAFSVFVQHVTPGAF
                     AAGITLLTGAPELSIAIIKAGLFGLTASVIACYKGISVGGGPAAVGNAVNETVVYSFL
                     SLFVIDVIVTAVGIKATA"
     gene            4956..5813
                     /locus_tag="VGP27_19680"
     CDS             4956..5813
                     /locus_tag="VGP27_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853554.1"
                     /translation="MSYSVSSPRFPRLMRDVGGVVSGWKGIGAQARFFAQTLVAVGDA
                     LKDYRGETLRMIAQMGLGAGALAVIGGTVGIVGFLTLTAGSLVTIQGYSSLSDIGVEA
                     LTGFVSAYVNGRIVTPLTAVIALSATIGAGTTAQLGAMRINEEIDALEVMGIRSVAYL
                     ASTRVVAGVIVVIPLYCIAVWMSFMSARFATTVYYGQPTGVYDHYFSTFLQPTDILWS
                     LFQAVLMGLVVMLVHTYYGFTASGGPAGVGEAVGRAVRTSLVVAVIVILFASLAIYGR
                     TGNFHFSGG"
     gene            5895..7289
                     /locus_tag="VGP27_19685"
     CDS             5895..7289
                     /locus_tag="VGP27_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853555.1"
                     /translation="MRFMGGDDKYLGWWTTILVTVVVALIALSWALFNGSFRSSVPVT
                     LTSDRSGLVMEPGSKVRFRGVQVGQVDSVSAGTESVKLQLEIDPDVIDTLPANTSAQI
                     RATTVFGAKYVDLIYPDQPQAQRLAAGAVLQSRNVSTEVNTVFQNLLLVLKQVDPAKL
                     NATLTALAEGVRGQGEVIGAATTDANEVLLEINPRMDTVRQDLRSLSGFSRAYGGAAQ
                     DILRTLSAVSTTSATVAGRSADLDALLVNVIGFAQAGTNLIQPSQSDLVRAVNVLDPT
                     TNLLMKYSPSYTCMLLGATWFLDKGGRDALGAGDGRSGILDATFLLGDDEYKYPENLP
                     VVAAKGGPGGKPGCGSLPDASKNYPVRQLISDTGWGTGLDVRPNPGIGHPWYANYFPV
                     TRAIPEPPSFRGEGPPAIGPVPYPGAPPYGAPEYGPDGTPLYPAPPGTPPAPPLQGSP
                     PAVPTPPDVSGTGP"
     gene            7286..8314
                     /locus_tag="VGP27_19690"
     CDS             7286..8314
                     /locus_tag="VGP27_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853556.1"
                     /translation="MKDDLVGALWRLSIFVTVCLLTLFGLYAVFGQVRFQKEKSYNAE
                     FSNVGGLEEGNFVRIAGVEVGKVKKVAVKQNSFAVVEFTADDSVVLTQGTKAVIRYDN
                     LIGGRFLALEEGAGPLNRLEPGQTIPLSRTAPALDLDALIGGFRPLFRALDPEQVNAL
                     TGQLISAFNGQGATVGSFLAQTSALTNTLADRDDLIGQVIVNLNNVLASLGDQSDKFG
                     SAVDSLSGLVKGLAERRTDVSRGLANLDRGAASVADLLSEARPPLQNTVAQSDRATSI
                     VVADHAYFDDLLNTLPDAYQQLGRQGLYGDFFSFYLCDLVLKLNGKGGQPVFVKLAGQ
                     TTGRCAPR"
     gene            8311..9561
                     /locus_tag="VGP27_19695"
     CDS             8311..9561
                     /locus_tag="VGP27_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853557.1"
                     /translation="MRSFGERNPLVIGAVGCAVTVGIMVGATQYDKLPFFSGGEVYTA
                     YFAEAGGLRSGAAVQVFGARAGQVSSIKLDGPRVLITFDLSKDIELGDRTEAAVKTKS
                     LLGTKILEITPRGVGKLSGAIPLDRTTPAYQLPDALGDLTAAISGLNTDQVSQSLSTL
                     AETFAETPPELRAAVSGVARFSQAVDVRDGQLRTLLSNANKVTAVLSERSELIVNLVS
                     NTNALMAELQKESAALRTVSANISALSRQLSGLVADNRTQLRPALDKLNGVLTILDNR
                     KGRVQTSIKLLNSYITSLGEAVAAGPFFKAYVANLLPGQFIQPFVDAAFSDLGLDPSV
                     LAPSKRSDPEIGQPGTPALPVPFPRTGQGGEPNLTLPDAITGKPGDPRYPYDPPLPQP
                     APGGPPPGPPAGYVPDTAPTQATP"
     gene            9558..10874
                     /locus_tag="VGP27_19700"
     CDS             9558..10874
                     /locus_tag="VGP27_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853558.1"
                     /translation="MTALRKFRTGLAGALVLLLVAAVLTVIPEARHADNVTVTAYFDN
                     SNGIFVGDEVQILGVPVGEINTIEPQPGRAKVTFSYDGHFQVPADAQAAILSPALVTS
                     RAIQLTPAYTTGPTMADGAVIPEERTAVPVEWDDFRAQLDKLTQSLQPTQPGGVAPLG
                     SLINTAADNLRGEGANIRDTIIKLSQAISVLGDHSTDIFSTVKNLSILVSALQGSTDL
                     MRQVNRNVASVTALLAADPNAVGNAIKDLNVAVADVQGFVAENRDSLGTTSDRLASVS
                     KAVTESLDDVKQTLHVAPTALNNYNNIWQPAQGALSGALMLNNFANPITFICGAIQAA
                     ARLNAEQSAKLCVQYLAPIVKNRQLNFPPLGENLFVGAQARPNEVTYSEDRLRPDFKP
                     PTPEAPPTPEAPPVPEVNAPLLPAEAIPTHPDEGLSGLMVPQGGGS"
     gene            10871..12010
                     /locus_tag="VGP27_19705"
     CDS             10871..12010
                     /locus_tag="VGP27_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HEV7853559.1"
                     /translation="MRRPMRSVIAGGLVVFLAVTALSGCGWRGLNSLPLPGTAGSGPG
                     SFEVKAQLPDVTNIQQNSRVRLADVTVGNVVKIEREGWHSLLTMRINGNVELPANAVA
                     TIGQTSLLGSLHVELAPPTGVPPKGKLRNGSLIPLAAAGSYPTTEQTLAALSLVLNGG
                     GIGQVQDITRAFSTAFAGRESDLRSLIEQLDQFMGRLSAQSDDIIKATESVNDLVGQF
                     SDQRPVLDRALQTFPEALAVLKEQRENLVEVLDKLGKFSALTADTVDQTKEALIQELK
                     DLGPVLKSLADAGPSLTRSLSFLTTFPWNKETIPNWFRGDFANLTLVLDLTLSRLDSS
                     FLTGTRFEGGLTKLEMQWGRTIGQQPSPYTSVNPLTFPYQFDQGP"
     gene            12012..>12242
                     /locus_tag="VGP27_19710"
     CDS             12012..>12242
                     /locus_tag="VGP27_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7853560.1"
                     /translation="MHLERRVLIKLAVFAVISIIAVSVTVFGYVKVSNLIPGFGHYTV
                     TVQLPQSGGLYAGGNVTYRGTEVGRVEDVRVTG"
BASE COUNT         2222 a         3927 c         3871 g         2222 t
ORIGIN      
        1 gtcgctgcac cgctcgatgc aggaactgca actgctctcc accgagggcg agtcgcagga
       61 gccggcaatg gccggagtca gcgccagcta caacggccat ttcgtgcggt gcttcgacct
      121 gaccaacgag cattacgtca acgcgctcaa gggcaagaac cttgcggaca tgtcggcgat
      181 cgagcggatg cacctggtgc cgccgccggg aatgtcaccc gagatggtcg acggactgcc
      241 gaatcgattc gacgatgggc aactgaaggt cctcgccggg taccctccgc aggtcggtca
      301 gctgtggccg aatacggctg cgctctgcat gccgttccag ttccccgacg gcaccccgtc
      361 ggccttcctc agctggcggg tctgggtacc gagaggacca gagcacttcg agctgttcca
      421 ttggagcctc gtcgaacgcg acgcgcctgc cgaactccgc gacaccgtga acctgatgac
      481 ggccgccacc ttcggcatca gcggtttcgt cgaggctgac gacaccgaca catggcccat
      541 gcagacctac gcctccaagg gtgcgctggg ggcccagcag aagctgcgtt accaggcgat
      601 caccggagag aacaagccga gcgactggcc gggtcctgcg caggtctacg ccgggttccc
      661 caaggacgac acgcagtgga atttctggct gcgctacagc gagttcatgg aagggagagc
      721 gtggtgacca cagaagcaac tcgagacgag gtcgttcccg ccgttcgggc aaccatgggc
      781 accgtcgact atccgagcgc gaagggcgcg cgaatcaagg tgaccgaccc gcgtcacttc
      841 gagatcgttg cgtggctgga agacgagtcg accctgctcg acgacgacaa cctgaccggt
      901 tggcttgcgc tgctggcgcc cgacgtcatt tatcgggcac cggttcggac cacccgcgac
      961 caccggtcca agggggtgtt cgaagcggag atgtaccact tcaacgagaa catcatgacc
     1021 ctgatgttga agctgatgcg gttgacgcag accgatagcg catggtcgga gaatccgctg
     1081 tccagaacac agcggatcgt gcacaaggtg cgcgcatacg agaccgacaa ggccgacgag
     1141 tacgaggtct tcagctccat cgtgctcatc cggtcccgct atgacgaacc ggcgctcgag
     1201 ttcctgcctg ctcgccgggt cgacaccatc cgaacgggcg acgagtggaa gctcgcggca
     1261 cggaccatct atttcgatca agccacgctg ggtgtgcaga acctggccgt ctatctgtag
     1321 aaaggtctga attgtcttct gcaacagacg agatgcaggg ctacctgccc ggcatctggt
     1381 cgatcgatgc ggtgcggtcg accctgacgt tctcggtggg ccacctggga gtgcacaccg
     1441 tgcacggaac gctcggcgtg tcagggcaga tcaccatggc cgacgatccg ctcgattcgg
     1501 cagtggacgc gtcgatcgac ctcggccagg tcgacaccgg aagcaagggg cgcgacaacg
     1561 cgatccggtc ggcacacctc ctcaacgtgg ccagccggcc gacggcgacg taccggtccc
     1621 ggcgaatcgg catcgggacg atgcccggcg aattcctgct acacggggat ctctcgttgc
     1681 tgggcgtcac ccgggaggtg ccgctgcgga ttcgtctgga gcgctccacc gatggtgacc
     1741 ggcaccggcc ggtcgtcacc ggcacaggtg aattcgcccg tcgcgacttc ggtttcgtgt
     1801 accgggtgcg cccacggttc ctcgatcgcg ccatctcgtc gaccgtgggt gtcgaagtac
     1861 gactcgaggc gtcacccacc gacacaaccg aagggacagg acgctgaaga tcgcaagaat
     1921 ccgtcacgag gacgccgatg tctggggttt ggtggacgtc gacacggaaa ccgtgcagcc
     1981 gattcgcggt ggtatcgagc agtgggggcc ggcggcggcg gacggcaccg cggacttcga
     2041 gaaggtcggt cggccgttgg cgatgacgga cgttcgctgg ctgccaccga tcacaccgac
     2101 gtccaccatc gtcggcgtcg gcatgaacta ctggtcgcac ctggagaagc tgggcgtgac
     2161 cgagcgcccg aagagcaccc ttggcttcct caaaccgcag gtggcaatca tcggccacgg
     2221 cgacacgctg gccaatccgg cgatcacgga gcaattggac ttcgaggtcg aactcgttgt
     2281 ggtgatggcg gagtcagtga gtcgcgtcgg cggcgtgccc accgacgcgg tcctcggtta
     2341 caccgtcggc aacgacgtca gcgcgcgcga cgcccccgcg ccgatgagtg gtgggctgga
     2401 tctgttctcc atcaaggcgc tcggcggtac gacgccagtc gggccatgga tcaccaccaa
     2461 agacgagttc ggtggctcgg gccagatcga cgtcggcata tcattacgcg tcaacggtga
     2521 agaacgccag catgaccgca ccggcaagat gatcttcagc atcgacgaat gtctcgagta
     2581 cgtcgtcgcg aggatggcac tcaccgccgg tgacatcgtg ttcacgggca ccactcatgg
     2641 tgtcgggttc gaggacggcc gataccttga ggagggggac gtcgtcgaag cggaagtcga
     2701 gggagtcggc gtgctgcgca acgtggtcgg cgccagatcg acgtgagacg tcagcttacg
     2761 gctttgcggt ccgaacggac gcgggacttc ctgggttcga ggccggctga cgcaccgagc
     2821 acgacgagaa cgtctcggaa ctcccgataa tcgcgcgaag gcatgaagta atgcgaaaag
     2881 gccatccgca caatcaggtc cgcgatctcg tccagggtgg ccgatccacc gagtacggcg
     2941 gcgctgtcgc ccaaaaccgg ctccaaggca tcgcgtagca ccgtggacag ccgaggcgtt
     3001 acccgcttga tgtaacccag cacgaagccc ggttcgagct gaccgatctg caccgttgcc
     3061 gggttctcca gccagaaccg cagcatgacg tcgatgacga tctggaggcg gtcagccggc
     3121 tccgggttgc cctcgatcgc gtcttggaga actgcctgca gcttcgtgac gaaatgatcg
     3181 cccaatgact gaaggagctc gtctttgttc ttgaagtatc ggtacagcgt cccgaccgag
     3241 accccggcct cgtgcgcgat gtcgctcatg gcaagtttct tcggaccctg ccgcgcgatt
     3301 gcgtgcaggg cgccgtcaaa gatgcgggcc tcggtagttg ccctggtcgc caacggaagc
     3361 ctttcgtggt gaagcagccg agttgctcaa ttcatctctg attatatcgc aaatcgattg
     3421 attaaacgca tttagctatt tatgtattat tagttctatt cattttttat ccggcatttc
     3481 catgggattg acggattctc gcaagaatgc gtgaccgaag ccgccaggga gtgtgccggg
     3541 agctagcctc aaacagtcgg agcccctgcg aattccggcc ggatcaatgc catggcagcc
     3601 gggatgaatt gcacttgcga ccgccgcgcg gccgattcgg caatcgctac ggatttaaaa
     3661 gcggaattct ccatgtctgt ttccccatag cgcacgattc cggtccaacc gtcgcttcgc
     3721 ttccgcgctg caatcgattg gtcgcgagcc ctcggcgatt cgccaggatg gctgatgcgt
     3781 attcaacgag gcacgtacgc cagatccgtg ccgcggtggc gtcacggttt catcactggg
     3841 ccgcctgatc gacggcgcac cgcgccccga gaagacgccc cgaatgccat gaactccgta
     3901 ggcaagtgac acgcatcgat aggcgggttt gatgagttcg cgggtatcac tctgtcgcaa
     3961 tgcattacat cgcagaaccg cgtctcgacg actggaccgg cgccggcttc gccggacagc
     4021 ccgcggtcgg aagaaatcgg cccttgaccc attatagtta tgaaactaaa gtatctccgc
     4081 gatggcagtc catgcaagca cagtcggcgc ctcctcgcgt ggcggccgtc ggcatggatg
     4141 tccacgggtg tttgccggca ccggtgaagt gccgcgccgg aggagctcgc tttgatctcg
     4201 atcgatcgcg ccgccaagcc gctgcgcggg gtgggcgact tcttcgcgat ggcgttggac
     4261 acgttggtgc agattccccg gcgaccgttc gccacgcgtg agttggtgct gcagtcctgg
     4321 ttcgtggcca gggtttcgct cttaccgacg ctcatgttgt cgatcccgtt caccgtgctc
     4381 accgtgttca cgttgaacgt tctgctgctc gagttcggcg cgggagactt ctcgggaagc
     4441 ggcgcagccc tcgcaggtgt cacgcagatc ggaccactgg tgagcgtgtt ggtggtcgcc
     4501 ggtgccggcg cgacggcgat gtgtgccgat ctgggctcgc gcaccattcg cgaagaactc
     4561 gatgcgctgc gggtactcgg gatcaatccg gttcaggcgc tggtggtgcc cagggtgctg
     4621 gcggcgacgc tggtgtcgat gctgttgtcc ggtctggtga tcgtcaccgg cctggtcggc
     4681 ggcttcgcct tctcggtatt cgtccagcat gtgacacctg gcgccttcgc cgcaggcatc
     4741 acgttgctca ccggagcgcc cgagttgagc atcgcgatca tcaaggccgg tctgttcggc
     4801 ctgactgcca gcgtgatcgc ttgctacaaa ggcatttcgg ttggtggtgg cccggcggcc
     4861 gtcgggaacg cggtgaacga aaccgtggtg tactccttcc tgtcgctgtt cgtcatcgac
     4921 gtcatcgtga ccgccgtggg catcaaggca acggcatgag ctacagcgtt tccagcccgc
     4981 ggttcccccg actgatgcgc gacgtgggtg gcgtcgtctc gggctggaag ggcatcggcg
     5041 cgcaggcacg cttcttcgcg cagactctgg tcgcagtcgg cgacgccctc aaggactaca
     5101 gaggtgagac gctgcgcatg atcgcgcaga tgggcttggg cgccggagct ctcgcggtca
     5161 tcggcggcac cgtgggcatc gtcggcttcc tgacgctgac agccggctcc ctggtaacga
     5221 ttcagggcta cagctcgctc agcgacatcg gcgtggaggc cttgaccgga ttcgtctctg
     5281 cctacgtcaa cggccgcatc gtcacgccac tcaccgcggt gatcgcgttg tccgcgacaa
     5341 tcggcgcagg taccaccgcc cagctagggg cgatgcgcat caacgaggag atcgatgccc
     5401 ttgaggtcat gggcatccgg tcggtcgcat acctggcctc cacccgcgtg gtggccggcg
     5461 tgatcgtcgt gatcccgctt tactgcatcg cggtctggat gtcgttcatg tccgcccggt
     5521 tcgccacgac cgtctactac gggcagccga ccggtgtcta cgaccactac ttctccacct
     5581 tccttcagcc gaccgacatc ctctggtcgc tgtttcaagc ggtcctgatg ggtctggttg
     5641 tcatgcttgt ccacacctat tacggcttca ccgcctctgg cggtccggca ggagtcggag
     5701 aggcggtggg gcgggcggta cgtacttcct tggtggtcgc cgtgatcgtc atcttgttcg
     5761 cctcactggc catctacggg cggaccggca acttccactt ctccggcgga tagccatggc
     5821 gcccgcacac atcgcaccga gcgcaccgcg ttgctcctca gccgaaaacc cgagacgggt
     5881 ctgctggtag cgcaatgcga ttcatgggag gcgacgacaa gtacctgggg tggtggacga
     5941 cgatcctggt gaccgtcgtc gttgcgctca tcgcactgtc gtgggcgttg ttcaacgggt
     6001 ccttccggtc gtcggtgccg gtgaccctca cctcggaccg gtccggtctg gtgatggaac
     6061 cgggtagcaa ggtcaggttc cgcggagtgc aggtgggtca ggtcgattcg gtaagcgcgg
     6121 gaacggaatc ggtgaagctg cagctggaga tcgatcccga cgtgatcgac acacttccgg
     6181 ccaacaccag cgcgcagatc agggccacca cggtcttcgg ggccaagtac gtcgacctca
     6241 tctatcccga ccagccgcag gctcagcgtc tggccgcggg ggcggtcctg cagtcgcgca
     6301 atgtcagcac cgaggtcaac accgtcttcc aaaacctgct cctcgtgctc aagcaggtcg
     6361 atcctgcgaa gctcaacgcc acgctcaccg cgctggctga aggagtccgg ggccagggtg
     6421 aggtcatcgg cgccgccacg accgacgcca atgaagttct gctggagatc aatccgcgga
     6481 tggacacggt gcgtcaagac ctacgctcgc tgagtggatt cagccgcgcc tacggagggg
     6541 cggcccagga cattttgagg acgctgtcgg cggtctccac gaccagcgcg accgtcgccg
     6601 gtcgctcagc cgatctcgac gcactgctgg tcaacgtgat cggtttcgcg caggcgggga
     6661 cgaatctgat ccagcccagt caaagtgatc tggtgcgagc cgtcaacgtg ctcgatccca
     6721 ccacgaacct gttgatgaaa tacagcccgt cctacacctg catgctcctt ggtgccacgt
     6781 ggttcctcga caagggagga cgtgatgcac tgggcgcagg cgatggcagg tcgggcattc
     6841 tcgacgccac cttcctactc ggcgatgacg agtacaaata cccggagaac ctgcccgtcg
     6901 tcgcggcgaa gggtggaccg gggggcaagc ccggctgcgg atcgctaccc gacgcgagca
     6961 agaattaccc tgtccgccaa ctgatctcgg ataccggctg ggggaccggg ctcgacgtcc
     7021 gtccgaatcc gggcatcggc cacccgtggt atgccaacta cttcccggtc acccgcgcga
     7081 ttcccgagcc ccccagcttt cgtggcgaag gccctccggc catcggcccg gttccctacc
     7141 cgggggcccc gccgtacggt gcgccggagt acgggcccga cggaacgccg ctctacccgg
     7201 cgccgcccgg cacaccgccc gctccgccgt tgcaaggttc gccgccggcg gtgccaactc
     7261 cgccagacgt gtcaggaacg gggccgtgaa ggacgacctc gtcggtgcgc tctggcggct
     7321 gagcattttc gtgacagtgt gcctgctcac tctgttcgga ctgtacgcgg ttttcgggca
     7381 ggtgagattt cagaaggaga agtcctacaa cgctgagttt tccaacgtgg ggggactgga
     7441 agagggaaac ttcgtccgga tcgccggtgt cgaggtcggc aaggtgaaga aggttgccgt
     7501 caagcagaat tcgttcgcgg tagtcgagtt caccgctgac gattcggtgg ttctgaccca
     7561 aggcaccaag gcggtgatcc gatacgacaa cttgatcggt ggccggttcc tggccctgga
     7621 ggagggcgcc gggccgctga accggctcga gcccgggcaa accatcccgc tgagcagaac
     7681 cgctccggca ctcgatctgg acgcgctcat cggcggcttc cgaccgttgt tccgcgcact
     7741 ggaccccgaa caggtcaatg cacttacggg acaactgatc tccgcattca atggacaggg
     7801 cgcgaccgtg gggtcgttcc tggcgcagac gtcggcgctg acgaacaccc tggccgatcg
     7861 cgatgacctc atcggccagg taatcgtcaa cctgaacaac gtgcttgcct cactgggcga
     7921 tcagagcgac aagttcggct ccgcggtgga ctcgctctcg gggctggtca aaggattggc
     7981 cgagcgcaga accgacgtca gccgcggact ggccaacctc gacagaggcg cggcctccgt
     8041 cgccgacctg ctgtccgagg cacgccctcc gttgcagaac accgttgccc agtccgaccg
     8101 cgccacctcg atcgtcgtgg cggaccacgc gtatttcgac gatctgctca acacgctgcc
     8161 ggacgcctat cagcagctcg gccggcaggg actctacggc gacttcttca gtttttatct
     8221 gtgcgacctg gtgctcaagc tcaacgggaa gggcggccag ccggtcttcg tcaagctggc
     8281 cggccaaacc acaggaaggt gcgcgccgag atgaggtcct tcggagaacg caatcccctc
     8341 gtcatcggcg cggtcggatg cgctgtgacc gtcgggatca tggtgggtgc aacacaatac
     8401 gacaaactgc cgttcttctc aggtggcgag gtctacaccg cgtacttcgc ggaggcaggt
     8461 gggctgcggt cgggcgctgc cgttcaggtc ttcggcgccc gtgccggcca ggtgtcgagc
     8521 atcaagctgg acgggccccg ggtgctgatc acgttcgacc tgtcgaagga catcgaactc
     8581 ggagaccgga ccgaggcggc ggtgaagacc aagagcctgc tcggaaccaa gattctggag
     8641 atcacgcccc gtggagttgg caaattgtcg ggtgcgatcc ctctcgaccg gacgacgccg
     8701 gcgtaccagc tgcccgatgc gttgggcgac ctcacggccg cgatcagcgg cttgaacacc
     8761 gaccaggtgt cgcagtcgct gtcgactctc gccgagacct tcgccgagac gccgcccgag
     8821 ctcagggccg ccgtttcagg tgtcgcccgg ttctcccagg cggtcgacgt gagggacgga
     8881 cagctgcgca ccttgctgtc caacgccaac aaggtcacgg cggtgctgtc cgaacgcagc
     8941 gagctgatcg tcaatctggt ctcgaacacg aacgccttga tggcagagct gcagaaggaa
     9001 agtgccgcgc tgcgcaccgt gtccgcgaac atctccgcac tctctcgaca gctctccggt
     9061 ctggtcgccg acaatcgcac tcagctgcgg cccgcgctgg acaagctcaa cggcgtgttg
     9121 accatcctgg acaaccgcaa ggggcgggta cagacgtcca tcaagctgct caactcctac
     9181 atcacctcgc tgggagaggc agtcgccgcc gggccgttct tcaaggccta cgtcgcgaat
     9241 ctgctgcccg gacagttcat ccagccgttc gtggacgccg cgttctccga cctcggtttg
     9301 gatccgagcg tgctggcgcc gtcgaagcgc agtgacccgg agatcggcca gcccggaacc
     9361 cccgcgctgc cggtgccgtt tccgcgtacc ggtcagggcg gcgaacccaa cttgaccctg
     9421 ccggacgcca tcaccggcaa acccggcgat ccgcgctacc cctatgaccc accactcccg
     9481 cagccggcgc ccggcggccc tccgcccgga cccccggccg gctacgtgcc cgatacagca
     9541 ccgacgcagg caacgccgtg accgcactgc gcaaattccg cacgggcctg gccggcgcgc
     9601 tcgtcttgtt gctcgtggcg gccgtgctga ccgtgattcc cgaggctcgg cacgccgaca
     9661 atgtcaccgt cacagcgtat ttcgacaaca gcaacggaat cttcgtcggc gacgaggtac
     9721 agattctggg cgtgccggta ggcgaaatca acacgatcga gccccaaccg ggacgcgcga
     9781 aggtcacttt ttcctacgac ggtcatttcc aagtgcccgc cgacgctcag gcagccatcc
     9841 tgtcacccgc actcgtcacg tcacgagcca ttcagttgac ccccgcctac accaccggac
     9901 ccaccatggc cgatggcgcc gtcatccccg aagagcgcac agcggtcccg gtggagtggg
     9961 acgatttccg tgcacagctc gacaagctca cccagtcgtt gcagcccacc cagcccggcg
    10021 gtgtggcccc gctcggatcc ctcatcaaca cggcagccga caatctgcgt ggtgaaggag
    10081 ccaacatccg ggacaccatc atcaagttgt cccaagctat ttcggtactg ggtgaccaca
    10141 gcaccgacat cttcagcacc gtaaagaacc tgtcgatcct ggtttcggca ctccagggca
    10201 gcacggacct gatgcgtcag gtgaaccgaa acgtcgcctc cgtcaccgcg ttgttggccg
    10261 cagaccccaa cgccgtgggc aatgccatca aggatctcaa cgtagccgtc gccgacgttc
    10321 agggcttcgt cgccgagaat cgagattccc tcggcaccac ctcagacagg ctggcatccg
    10381 tctcgaaggc tgtgaccgag agcctcgacg acgtcaaaca gacactgcac gtcgccccta
    10441 cggcgctgaa caactacaac aacatctggc aaccggccca gggcgcgcta tcgggcgcgc
    10501 tgatgctcaa caacttcgcc aacccgatca ccttcatttg cggtgccata caggcggcgg
    10561 cacggctcaa tgccgaacaa tcggcgaagc tctgcgtgca atacctggcc ccgatcgtca
    10621 agaatcgtca gctgaacttc ccgccgcttg gcgagaacct cttcgtcgga gcccaggctc
    10681 gccccaacga ggtcacctac agcgaggacc ggctgcggcc ggatttcaag ccccctacgc
    10741 ccgaggcgcc gccgacgccc gaagcgccgc ccgtacccga agtgaatgcc ccactgctgc
    10801 ccgccgaggc gattccgacg catcccgacg agggcctgtc cggcctgatg gttccacaag
    10861 ggggtggctc atgagacgac cgatgcggtc ggtgattgcc ggcgggctgg tggtgttcct
    10921 cgcggtgacg gcgttgtctg gctgtgggtg gcgcggcctg aactcgttac cgctgcctgg
    10981 caccgcggga agtggtccag gttcgttcga agtgaaggca cagctgcccg atgtcaccaa
    11041 catccagcag aattcgcgcg tgcggttggc tgatgtcacg gtcggcaacg tggtcaagat
    11101 cgagcgcgaa ggttggcatt cgctgctgac catgcggatc aacggcaacg tggagttgcc
    11161 tgcgaacgcg gtcgccacga tcggccagac cagtctcctg ggctcgctgc acgtggagct
    11221 cgcgccgccg accggtgtgc cgccgaaagg caagctgcgc aacggatcac tgatcccgtt
    11281 ggcggccgcg ggcagctatc cgacgaccga gcagacgctg gcggccctgt cgctggtact
    11341 caatggcggc ggtatcggcc aggttcagga catcaccagg gcgttcagca ccgcgttcgc
    11401 cggccgggag agcgatctgc gtagcctgat cgagcagctg gaccagttca tgggccgact
    11461 cagtgcgcag agcgatgaca tcatcaaggc caccgagagt gtcaacgatc tcgtcggcca
    11521 attctccgat cagcgacccg ttctcgaccg ggctctacag acctttcccg aggccctggc
    11581 ggtgctcaag gagcagcgcg agaatctggt cgaggtgctg gacaagctcg gcaagttcag
    11641 tgcgctgacc gcggacacgg tcgatcagac caaggaggct ctgattcagg agctcaagga
    11701 cctgggacct gtgctgaagt cgctggctga cgcgggcccg tcgctcaccc ggtcactgag
    11761 cttcctgacg accttcccat ggaacaagga gacgatcccc aattggttcc gtggtgactt
    11821 cgccaacctg acccttgtcc tggatttgac gttgagtcgg cttgattcgt cgtttctcac
    11881 cggcacccga ttcgaggggg gattgaccaa gctggagatg cagtgggggc gcaccatcgg
    11941 ccaacagccc agcccctata cgtcggtcaa tccgctgacg ttcccgtatc agttcgacca
    12001 aggaccctag cgtgcacttg gagagacgtg ttctgatcaa actggctgtg ttcgcggtga
    12061 tctcgatcat tgccgtgtcg gtgaccgtct tcgggtacgt caaggtgtcg aacctgattc
    12121 ccggcttcgg gcattacacg gtgaccgtgc aactgcctca gtcaggaggt ctgtacgcgg
    12181 gcgggaatgt cacctaccgc ggaactgaag tgggtcgggt agaggacgtc cgagtaaccg
    12241 gg
//
LOCUS       DASYLX010000841         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1585602,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000841 DASYLX010000000
VERSION     DASYLX010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585602"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGP27_19715"
     CDS             <1..366
                     /locus_tag="VGP27_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEV7853561.1"
                     /translation="ERDAGESVERQVVAAARPALDAWHRRGLDGTVKFGDNEAPAQMM
                     AGILSLELLVHAWDYAKALGRNVSAPDSLSEYVMGVAQKVITPQGRTTAGFDDPVNVA
                     AEASALDRLVAYTGRNPHG"
     gene            complement(445..1503)
                     /gene="hisC"
                     /locus_tag="VGP27_19720"
     CDS             complement(445..1503)
                     /gene="hisC"
                     /locus_tag="VGP27_19720"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV7853562.1"
                     /translation="MPARLRPELADLPPYTPGRNVPGAIKIASNETVHGPLPSVRTAI
                     AKAADNINRYPDNGYVELKERLAKHVNFAPEHIAVGCGSVSLCQQLIQITSTVGDEVI
                     YGWRSFEIYPLQTRTAGATAVQVPLTDDTYDLDAMLAAVTDRTRLIFVCNPNNPTSTV
                     VDPDALARFIEAVPSDILIALDEAYVEYITDDMLPDSFGLVRAHSNVVVLRTFSKAYG
                     LAGLRIGYAVGDPDIITALGKVYVPFSATSISQAAAIASLDAADELLARTDAVIAERA
                     RVTAALRDAGYSVPPSQANFVWLPLPGRAQEFAGDSANDRIIVRPYGDDGVRVTVASV
                     QENDLFLDFAKRWIAERT"
     gene            1588..1676
                     /locus_tag="VGP27_19725"
     tRNA            1588..1676
                     /locus_tag="VGP27_19725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1622..1624,aa:Ser,seq:gct)
BASE COUNT          285 a          541 c          593 g          290 t
ORIGIN      
        1 gagcgcgacg cgggcgaatc ggtggagcgt caggtcgtcg cggccgccag gcctgcgctg
       61 gatgcctggc accgccgcgg gctggacggc accgtgaagt tcggcgacaa cgaggcaccc
      121 gcgcagatga tggccggcat cctgtcgctc gaactcctgg tacacgcgtg ggattacgcg
      181 aaggcgttgg ggcgcaacgt gagcgccccc gactccctgt cggagtatgt gatgggcgtg
      241 gcacagaagg tgatcacgcc gcagggccga accaccgccg gtttcgacga tccggtgaac
      301 gtggccgccg aagccagtgc actggaccgg ctggtggcct acaccggccg caacccgcac
      361 ggctagccac accgccggcg ccgacttctg cagctgggtc gtggatctcg cgcacctaca
      421 gccctcgtgc agaattcggc acgattacgt ccgctcggcg atccagcgct tcgcgaagtc
      481 aaggaacagg tcgttctcct gcaccgacgc gacggtcact cgcaccccgt cgtcgccgta
      541 aggccggacg atgatgcggt cgttcgccga atcaccagcg aactcctggg cccgacccgg
      601 caacggcaac cagacgaagt tggcctgcga cggcggcacc gaatagccgg cgtcgcgcag
      661 cgccgcggtg accctggccc gttcggcgat gacggcgtcg gtgcgggcca gcagttcgtc
      721 ggcggcgtcc aacgatgcga tcgccgcggc ctgcgaaatg ctcgtcgcgc tgaacggcac
      781 gtacaccttg ccgagcgcgg tgatgatgtc gggatcgccg acggcgtagc cgatacgcag
      841 ccccgcgagc ccgtatgcct tggaaaatgt gcgcagcaca acgacattgc tgtgcgcgcg
      901 gaccaatccg aagctgtcgg gcagcatgtc gtcggtgatg tactcgacgt aggcctcgtc
      961 gagcgcgatc aggatgtcgg acggcaccgc ctcgatgaag cgggccaacg cgtcgggatc
     1021 gacgacggtg ctcgtcgggt tgttcgggtt acagacgaag atcagccggg ttctgtcggt
     1081 gacagcggcc agcatcgcgt cgaggtcata ggtgtcgtcg gtcaacggca cctgcaccgc
     1141 ggtggcaccc gcagtgcgtg tctggagcgg gtagatctca aagctgcgcc agccgtagat
     1201 cacctcgtcg ccgacggtcg aggtgatctg gatgagctgt tggcacaggc tgaccgaccc
     1261 gcatccgacg gcgatgtgct cgggcgcgaa gttgacgtgc ttggcgagcc gttccttcag
     1321 ttcgacatag ccgttgtccg ggtaccggtt gatgttgtcg gcggcctttg cgatagcggt
     1381 ccgcacactc ggcagcggcc cgtgcaccgt ctcgttgctc gcgatcttga tcgcgcccgg
     1441 cacgttcctg ccgggtgtgt acggcggcag atcggccagt tcagggcgta ggcgggcagg
     1501 caccgaacca gtctatgggt ggcgattatg cgctttgcct ggtgccgtgg gggatgtgta
     1561 cgctgtcgga tcggcggtaa accgtcggga ggcgtgccag agcggccgaa tggggctcac
     1621 tgctaatgag ttgtcgccct caaaggcgac cggaggttca aatcctctcg cctccgccgc
     1681 ggctgacgcg tcagccacgc acaactgaa
//
LOCUS       DASYLX010000842         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2901394,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000842 DASYLX010000000
VERSION     DASYLX010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901394"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGP27_19730"
     CDS             <1..1111
                     /locus_tag="VGP27_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEV7853563.1"
                     /translation="AFLLAGYGVDLIIAAKLVCVAIWMGAATSKLNKHFPFVISTMMS
                     NNPLVRTKWLKRKFFERFPDDLRPGRVSRYIAHFSTAIEMLVPVALLFSHGGWPTAIA
                     AFVMVCFHFGILSAIPMGVPLEWNVFMMFSVIALFVGHAEVGLTDMTTPLPVLLFAVL
                     ATVVVLGNLFPRKISFLPGMRYYAGNWDTTWWCIKPSANEKIERGLVSIASMPASQLA
                     KFYGSEEQALIFLYKGYAFRGFNSHGKALLTLVHHALAGRNQDDYLITEGERLCSTAV
                     GWNFGDGHMHNEQLITAMQERCHFERDEVRVILLDAQPFHRQRQEYRLVDAATGEFER
                     GYVNVADMVTGQPWSDDIPVNVTWSAGGQIKAPQT"
     gene            complement(1121..1729)
                     /locus_tag="VGP27_19735"
     CDS             complement(1121..1729)
                     /locus_tag="VGP27_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV7853564.1"
                     /translation="MTTTLTRAAALLTCLVAVLFLSACAKSAPAGDAGTTQDQRPVIT
                     GKPADHNAADIAFASDMIPHHQQAVDMSALVPDRSADADVQKLASDISAAQTPEIQTF
                     KVLLVQWDENPDLDNGSGKGGQSGMHGMVDEATMARLTSLEGAEFDQLWLQSMIGHHQ
                     GAIEMAKTEIANGANVDAKSIAQNIVTTQQAEIDQMNQLLGG"
     gene            1883..>2560
                     /locus_tag="VGP27_19740"
     CDS             1883..>2560
                     /locus_tag="VGP27_19740"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920476.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7853565.1"
                     /translation="MAEAVIVEAVRSPVGKRNGGLSGVHPAELSAQVLNGLAERTGID
                     PGIVDDVIWGCVMQAGEQALDIGRTALLTAGWPESVAGVTIDRQCGSSQQSIHFAAAG
                     VVAGHYDAVVAGGVESMSRTPMGASLAGGGNPYPQGFKERYKTTPNQGIGAEMIAEQW
                     GFDRTAVDEFSLGSHEKAAAAQDSGAFDDQIVAIKDQDGNPVLKDEGIRRGTTIEKMA
                     TLKPAFKE"
BASE COUNT          438 a          790 c          835 g          497 t
ORIGIN      
        1 cgcctttctt ttggccggct acggagtcga cctgatcatc gccgccaagc tggtatgcgt
       61 cgcgatctgg atgggtgccg cgacgtcgaa gctcaacaag cacttcccgt tcgtgatctc
      121 gacgatgatg tcgaacaatc ctctggtccg aacgaagtgg ctgaagcgca agttcttcga
      181 gaggtttccc gacgatctgc ggccgggccg ggtctcgcgg tacatcgcgc atttcagcac
      241 cgcgatcgag atgctggtgc cggtggcgct gctgttctcc cacggcggct ggccgacggc
      301 gatcgcggct ttcgtcatgg tctgcttcca cttcggcatc ctgtcggcga tcccgatggg
      361 ggtgccgttg gagtggaacg tcttcatgat gttttcggtg atcgcgttgt tcgtcggtca
      421 cgccgaagtg ggcctgaccg acatgaccac cccgcttccc gtgttgctgt tcgccgttct
      481 cgccaccgtc gtggtgctcg gcaatctgtt cccgcgcaag atctcgttcc tgccgggaat
      541 gcggtattac gcaggcaact gggacaccac gtggtggtgc atcaagccgt cggccaacga
      601 gaagatcgag cgcggtctgg tttcgatcgc cagcatgccc gcctcccagc tcgcgaagtt
      661 ctacggaagc gaggagcagg cgctgatctt cctgtacaag ggatacgcct tccgcgggtt
      721 caactctcac ggtaaagccc tgctgaccct cgtgcatcac gccttggcgg ggcgcaatca
      781 ggacgattat ctgatcaccg agggagagcg gctgtgcagc accgccgtcg ggtggaactt
      841 cggcgacggc cacatgcaca acgagcaact gatcacagcg atgcaggagc gttgccactt
      901 cgagcgggac gaggtgcgtg tcatcctgct cgatgcgcaa ccgttccacc ggcaacgcca
      961 ggagtatcgc ctggtcgatg ccgcgacagg ggaattcgag cggggttatg tgaacgtcgc
     1021 cgacatggtg acgggtcagc cgtggtccga cgacattccc gtgaacgtca cgtggtcggc
     1081 cggtggtcaa atcaaagcgc cgcagacctg actcctcgga ttagcctccg agcagttggt
     1141 tcatctggtc gatctcggcc tgctgcgtgg tgacaatatt ctgggcaatg ctcttggcgt
     1201 cgacgttggc gccgttggct atttccgtct tcgccatctc gatggcgccc tggtgatgcc
     1261 cgatcatcga ctgcagccac aactggtcga actcagcgcc ctccaaggaa gtgagtcggg
     1321 ccattgtggc ctcgtcgacc atgccatgca ttccgctctg gccgccctta ccgctaccgt
     1381 tgtccaggtc cgggttctcg tcccactgca ccagcaggac cttgaaggtc tggatctcgg
     1441 gcgtctgtgc ggccgaaatg tccgaggcca gcttctgcac gtcggcgtcg gccgagcggt
     1501 ctgggacgag agctgacatg tcaacggcct gttggtgatg cggaatcatg tctgacgcga
     1561 acgcgatgtc ggcggcgttg tggtcggcgg gcttcccggt gatcacgggc cgctggtcct
     1621 gcgtggtgcc ggcgtcgccg gccggtgccg acttcgcgca ggcagacagg aacaagacgg
     1681 ctaccaaaca ggtcagcagc gccgccgccc gggtcagggt ggttgtcatg acgtcaccgt
     1741 accgagcacg gtcctggccg agaccacgct ggtgcacctg tgtgacctac caaccagtag
     1801 gttgacctag taaaccttgt tgctctacgt tcgataatgg agctctgtgg gagaacccac
     1861 caaattttga aggagaagtg tcatggctga agccgtcatc gtcgaggcag tgcggtcacc
     1921 cgtcggcaag cgcaatggtg gactgtcggg ggtgcacccg gcggagcttt cggctcaggt
     1981 cctcaacggt ctcgccgagc gcaccggcat cgaccccggc atcgtcgacg acgtcatctg
     2041 gggctgcgtc atgcaggcgg gcgagcaggc gctcgacatc ggccgcaccg cgctgctgac
     2101 cgcgggctgg ccggagagcg tggccggtgt gaccatcgac cgccagtgcg gttcgagcca
     2161 gcagtccatc cacttcgccg cagccggtgt ggtggcagga cactacgacg ccgtggtcgc
     2221 cggcggcgtc gagtcgatgt cgcgcacgcc gatgggcgcg tcgctggccg gcggcggcaa
     2281 cccgtacccg cagggtttca aggagcgtta caagacgacg ccgaaccagg gcatcggcgc
     2341 cgagatgatc gccgagcagt ggggcttcga tcgcacggcg gtcgacgagt tctccctcgg
     2401 ttcccacgag aaggccgccg cggcacagga ttccggtgcc ttcgacgatc agatcgtcgc
     2461 gatcaaggat caggacggca atccggtgct caaggacgag ggcatccgtc gcggcaccac
     2521 catcgagaaa atggccacgc tcaagcctgc gttcaaggag
//
LOCUS       DASYLX010000843        16262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1804404,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000843 DASYLX010000000
VERSION     DASYLX010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804404"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGP27_19745"
     CDS             complement(<1..819)
                     /locus_tag="VGP27_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853566.1"
                     /translation="MSEPDWLDVATPSVQLRALTWGRPDDPIALCLHGFPDTAHGWRK
                     VAPALVDAGWRVVAPFMRGYAPSSIPADGSYHVGTLMNDALQVLDAAGPTGRDVVVGH
                     DWGAATAAGLAAMPDSPFAASVIMSVPPFAAYQPWGRAPDQLRLVAQIPRQLLRSWYI
                     MYFQLPWLPEFSPSRVVPMLWRQWSPGYDQADDDVALVQDAIGAQENWLAAISMYRQN
                     FRGTKPPAEYADLHPLFLKPPLMPFLYLHGDDDGCMAPDYIAWVERILPSGSDAS"
     gene            complement(833..1507)
                     /locus_tag="VGP27_19750"
     CDS             complement(833..1507)
                     /locus_tag="VGP27_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853567.1"
                     /translation="MTDTPKDKTAEEETTVRVDEEPTEEATPSESDEKSDSNTLTDAT
                     DEPADATEAADVAEPAKRRRLDSRRVIAFAVLPALALLLALGAAFLKFQDSAVRGSDV
                     ARDESVQAAKDSTVALLSYKPDTVDKQLVAARDLLTGDFRDSYTSLTNDVVIPGAKEK
                     QISAVAKVPAAASVSANPGRAVVLVFVNQTVSVGNGVPTDTASSVRITLDRVGDRWLI
                     SKFDPV"
     gene            complement(1504..2178)
                     /locus_tag="VGP27_19755"
     CDS             complement(1504..2178)
                     /locus_tag="VGP27_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mce protein"
                     /protein_id="HEV7853568.1"
                     /translation="MAKHGVDPDAGAKEPTDAETAVTETADGTTTDAEAQSTAVEAEE
                     TEHLDADDADDVESKVGRPRMSPVRLATILGLVAVVALAALTGWLGFRAYESRKAADE
                     RNLFLQVGRQGALNLTTIDWEHADSDVQRILDSATGSFYDDFQNRSAPFVQVVKQAKS
                     KSVGSVTEAGVESESGDEAQVLVAVTVKTSNAGAAEQEPRSWRMRITVQKMGDQAKVS
                     NVEFVP"
     gene            2364..3185
                     /locus_tag="VGP27_19760"
     CDS             2364..3185
                     /locus_tag="VGP27_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853569.1"
                     /translation="MRVIVTGGNSGVGKATATAMAAAGHDVVIACRTPSKAHQAAASI
                     AGNVEVAELDLADLASVRKFADSVDFVDVLVNNAGVLGLPLTRTADGFEAHMGTNHLG
                     HFALTCLLGDRVRDRVVAVASSNHALAKMHFDDINWHRRRYNPWSAYGESKLANVLFV
                     QELARRNRTAYASDPGMTATDITRDGSGLLQWAGRTLSPHIAQSPADGARSTIEAVTT
                     DLPNGTYIAPRGLFHQWGKPKSTKLAAKARDPESARRLWDLSAELTGCDWQGAGA"
     gene            3182..3637
                     /locus_tag="VGP27_19765"
     CDS             3182..3637
                     /locus_tag="VGP27_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7853570.1"
                     /translation="MTTDHTYRRPPTRLAPTPTPESMAFWTGGRNGELLINRCHGCGR
                     FFHPPGPACWRCRSTDVAPEPVSGRGTVAAYTINRQPWIPGLDPPYIVAMVELADEPD
                     VRLITNVVGVSGDDIRVGLPVEVFFEDWTAMSGEEDSRVWLPLFRPSPD"
     gene            3735..4856
                     /locus_tag="VGP27_19770"
     CDS             3735..4856
                     /locus_tag="VGP27_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7853571.1"
                     /translation="MTGFAANVTLNSPADLTPEILTSLLRRHHPDVTVTDVRLRKSWQ
                     GTTSHLHLDVDYAEPESVLPQQLFMKTQLGTVHDLPTAVDESLSEGGGGTVLLDDETT
                     FYRDLRDDLDVETMTTYFAEHLDGPSQFVIIAEDITLRGAEVPDAVAGLSVEQVDQLL
                     ATLSRTHAPFWASPRLKEGGDLSWLQNPVTGRFADFLRTSGFAIIRAFLELPYKRELL
                     ESAGTDIDAMEAAFWALQDRVAVEPITLLHGDPHPRNTYVLPDGSVGLLDWQLVRRGS
                     WVHDVGYALIAALPPALRRDHERELLDNYRRRLIDSGVDAPPDREQMWTAYRRSPAWG
                     FCMWAITPDQMYSVEIVRAVLGRFAEAYSDLGTGALLTS"
     gene            complement(4883..5887)
                     /locus_tag="VGP27_19775"
     CDS             complement(4883..5887)
                     /locus_tag="VGP27_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV7853572.1"
                     /translation="MSNTIGQLSSGLSADSERTVQECPDIPAWTENLLFTPYDPVNDV
                     GMWLHLGTVADKWEMWEDRVLIALPQDQGVLSMWAYHRTIPERRPAGANLEFHCEEPF
                     RKWRITFDGFCLRSSYEQMRTAPLADGSKSHVRMDLNVECVTPVWDAATAGEGMAEQS
                     WAREHYEQLTRVTGQLVVDGVSIDFNGVGWRDHSRGPRGADTLKDWGGHVIAGAVLPS
                     GRAFGLCRYWARDGHTTLEGAYLVDDGVLRHVDITSVPPPAVHARHGETFSFELGGEH
                     EIACAVRSSMWMSMSDGLPYGVTDPVRAYTVSWAELQWAGESGHAYLERSAMTASSVQ
                     "
     gene            complement(5900..7513)
                     /locus_tag="VGP27_19780"
     CDS             complement(5900..7513)
                     /locus_tag="VGP27_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEV7853573.1"
                     /translation="MADDGTPAGLTDPIRGRSEPDRMWTLTNVGEATPDILSPLCWSL
                     WGDGVECASRAGIYDFGVLSRSALAVPDDPNQWATACFYGRQAMNVDRARELAGLLPG
                     TTGDDFERDLLGSVRADATPTKSDPLRIPFVAVKAPLAAIRQRWAPQRAYDDQMDWWR
                     SEVLPGDPVDPSTLLADSVRRFTEVMRVHIRTRMILNTLRAQIESIAGRLGHADLVPT
                     LLAGYGGVIETGLADDVWIVGQGRLSVAEFVARHGFHGPNEGNVIGHSWREDPEAVER
                     IAAVHADRPEAERPQARAKHAVAARKQAESELLAGLSRLRRVALRGLLQFTGFQVRSM
                     ELTKAAFLTAIDGTRAAAGALGRELVRAGKLDSVDDTFYLTTAELLGPLPYNARELVA
                     FRRQRREEYRALDIPVSFVGMPVPIEVNDTAGTDDEGVRGMPAGPGIVEGTVRVVLDA
                     DGDDVLDDGEVLVCKFVDPGWTALVSLAGALVTDIGSPASHGAIVARELGISCVVGTG
                     NGTKVLRTGDVVRVDGSTGEVAVLARSTG"
     gene            7652..9313
                     /locus_tag="VGP27_19785"
     CDS             7652..9313
                     /locus_tag="VGP27_19785"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEV7853574.1"
                     /translation="MRFTVTHGSANRGGSSTGLDVDPTSVALLDGSCPLSRQELGNKA
                     HGINLIRRLGLPVPPAFCLTTAVCTAFLSGDQRALDRVWPDVLESLRWLERETSRTFG
                     RGPRPLLLSVRSGAAQSMPGMLDTVLDLGMDDDVQDAMASSATPGFASDTRARFDSMY
                     RRIVLGSDESADIPDDPFLQLRGAIEAVFASWNSPRATAYRARHGLDGNAGTAVVIQA
                     MVFGNLGGHSGTGVLFSRNPVTGADEPLGEWLPCAQGEDVVSGTYDCLPLNALRDAQP
                     DVYGELLTAAAELERFGRDVQDVEFTVEEGRLWLLQTRVAKRSPQAAVRLALKFRSSG
                     VIDDLETAQRVTPDHVRALLSPSLQPDTCSAATLLAKGLPASPGVAFGTAHTDIDDAI
                     DAADAGEDVILVRISTSPDDVAGMLAAKAVVTEIGGATSHAAVVSRELGLPAVVGCGA
                     GVAAALEGRSVTVDGGAGEVYDGALEVAAWSERDSPELTELTAIALRVSPLRVHTAGA
                     HPKLSATTEAAVHEAVSAGRTDVVSDAPLIAMLTAVALAGRSEDG"
     gene            9306..9911
                     /locus_tag="VGP27_19790"
     CDS             9306..9911
                     /locus_tag="VGP27_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853575.1"
                     /translation="MDELALLQAIRLKGRVRPAGLAATLQQDEDVVATAVEDLAEAGL
                     VVRGTAVRLTPEGKDRLAEFLAEERNGIDGHAITRVCDDFRTVNREFKALVSDWQLRN
                     GGPNDHSDADYDAAVLGRLEGVHGAILPILACASEQLPRLNTYSAKLSTAFVKIQGGD
                     PTWLTRPLVDSYHTVWFELHEELIGAAGLTREDEAGDGHAG"
     gene            complement(9981..10805)
                     /locus_tag="VGP27_19795"
     CDS             complement(9981..10805)
                     /locus_tag="VGP27_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7853576.1"
                     /translation="MGRMDGRVAFITGVARGQGRSHAVRLAEEGADIVGIDICRQLDG
                     VLYSLASPEDLDETVNLVEKTGRRMVARQADVRDRDALAAAFADGVAEFGHIDTVVAN
                     AGILLNRVDEPDPVAAWELGIGVMLTGVWNTMQIATEHMIEAGRGGAMIVTSSMAGLR
                     ALTDGTGGSDAYAAAKIGVTGLVRAYAQRLAPNNIRVNAIAPTGVATTMIVDNPALFE
                     VIAKHEHLANAFKNSLPVQLIEPGDVSETVLFLAANDSGRYYTGSTLMVDAGMNIA"
     gene            complement(10815..11234)
                     /locus_tag="VGP27_19800"
     CDS             complement(10815..11234)
                     /locus_tag="VGP27_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7853577.1"
                     /translation="MTNPQLLPPIPVPDPDTAPFWDGLKDGRLMLCRCDDTGKWIHPP
                     LERSRFTGGPVHFEEVGGEGTVFSYIVIRQALVPGRTPPYVIALVELAEQPGLRISAV
                     LDLDPADVRIGQPVKLRIADLGESGFRIPEFVVKESA"
     gene            complement(11231..12367)
                     /locus_tag="VGP27_19805"
     CDS             complement(11231..12367)
                     /locus_tag="VGP27_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nonspecific lipid-transfer protein"
                     /protein_id="HEV7853578.1"
                     /translation="MTCAVVGIGRTTYSRSSGRTTRAMAVSACRDAIADAGLTSADID
                     GICTYMANDSEQPINVGWALGIDELAWANAMYGGGNLVADQIATAAAVIEAGMCKAVL
                     VYRSLNGRSGYRFGHVEGPMQVPNHDQFDAASGFMVPPQWIAMWARRHQHEYGSTCED
                     MGQIAITQRAHAGPNEHALRREPLGMDEYLAARWINEPFRVLDCTSEVDGAVAILITG
                     EDMARDCKQPPMWLVGSSNSQGGSGWSEWDDPTEMFSRTAGPKIWANTGLTPSDMDVA
                     CMYDCFTYTVMATMEGFGFCEKGEVGKFFSTGRATYGGDVVVNPHGGLLSEGYIHGLN
                     HHFEAALQLRHAAGVRQVENAQLALVTAGAGPFGGANVYSREKP"
     gene            complement(12381..14531)
                     /locus_tag="VGP27_19810"
     CDS             complement(12381..14531)
                     /locus_tag="VGP27_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7853579.1"
                     /translation="MSLAITEDHRALAEVAKAMVAGRSGTAGARRILLEPESKGRWWH
                     DDGLWKEIVSAGWLGMHVDAQFGGEGYGLPELTIVLEQLGRAAIGGPFLPTVAVSSVI
                     AEVGTDEQRKRWLPGLTSGDVVAGIGTTSDAAQSDSTVNADSVAALAESAADVFLVPV
                     GEDLVLVEAGSAVTTRSVDSVDQIMRPITVLGLSSAPVADVFPGAAGVAARVLRILAA
                     SEAVGGLAACTEMATAYAATREQFGRPIGSFQAIKHHCANMLLDTELAVAVTWDAARA
                     HGEEAELAATMAAGHVLPAYQRVALQNVQVHGGIGYTWEHDAHLYIRRATVLLAFVGG
                     QETLRDKVAELQSGGLRRRHSVDLPPEAEQYRRAAEEFRAELSRADAADQQKIWARSG
                     YLQPHWPEPYGRDADSVEQLIIEEALDGLEKPSLGLGGWVVPTLLQHGSDELIERLMW
                     PSLEGELRWCQLFSEPGAGSDAAAVATKAKKVDGGWSVTGQKVWTSDAMNCQRGLATV
                     RTDPNVPKHKGITAMVIDLADPDVRIRPLKEITGETLFNEVFFDDVFVPDRDVVGAVN
                     AGWTVAMATLGNERVSIGGGSVTMTADALLELLARHRPDDGGLVRDVGALLIEAYAAA
                     TLNLRQAARAVFDTGPGIEGNIAKLFGAEHAQRVAELALRIAGPALMLGDEGNVLHDY
                     LFSRCLTIAGGTSEVVRNLIAERILGLPREPAPK"
     gene            complement(14560..15168)
                     /locus_tag="VGP27_19815"
     CDS             complement(14560..15168)
                     /locus_tag="VGP27_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7853580.1"
                     /translation="MGELLSVMHAQRACRRFDPDGKVDDADLERILESAVHAPSAENT
                     QPWSFVVVRNEDTRAQLAEWWTETWNAGGGDFVRQSVDDKVLVEDLEYGFTRGGFAAA
                     PVVVVVCADTERVPEIYAPSSIYPAVQNLLLAAADLGYGSCLTTGLTTFGVDRVREKL
                     QLPESLQPMAAVYLGKPARKLSPPRRRPAVSVTYREQFGTPW"
     gene            complement(15168..15533)
                     /locus_tag="VGP27_19820"
     CDS             complement(15168..15533)
                     /locus_tag="VGP27_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853581.1"
                     /translation="MAPNIFVVLTNPIEGEDDAFNKWYDAQHVPEVLDVPGVVAAQRY
                     DIAEVNVPDDEELPAQLPPPTHRYLVIYELDNDPDVVMSEFLKRVMAGTLSLGEWLDL
                     STISLTGWVPRGDRVVAGS"
     gene            15685..>16262
                     /locus_tag="VGP27_19825"
     CDS             15685..>16262
                     /locus_tag="VGP27_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7853582.1"
                     /translation="MRFAVYLPNCMHVGAITQPWEHGLTGPDVALTAQTAERLGYSMV
                     FLPEHFLTPTSHVELSGDHYFDATTAQAFVAGATSSITIGSMVTILPLHNPIIAAKTI
                     STLDWLSDGRAQVTVGVGWLKEEYDAIGVPFSKRGRIADESLAAMFELWHSDTPSFDG
                     EFVKFDDIAFGPKPIREPHPVIWMGGDADAVLR"
BASE COUNT         2777 a         5535 c         5113 g         2837 t
ORIGIN      
        1 agaggcgtca cttcctgacg gaagtatccg ctcgacccag gcgatgtaat ccggtgccat
       61 gcagccgtcg tcgtcgccgt gcagatacag aaagggcatc aaaggcggct tcaggaacag
      121 cgggtgcagg tccgcgtact ccgcgggcgg cttggtgccg cgaaagttct gccggtacat
      181 gctgatcgcg gcgagccagt tctcctgggc gccgatcgca tcctggacaa gtgcgacgtc
      241 gtcgtcggcc tggtcataac cgggcgacca ttgccgccac aacatcggca ccacgcggga
      301 cggggaaaac tcaggaagcc agggcaattg gaagtacatg atgtaccagc tgcgtagcag
      361 ctgccgggga atctgcgcca ccagcctgag ttgatcgggc gctcgacccc acggttgata
      421 cgccgcgaac ggcggcaccg acatgatcac cgacgcggcg aacgggctgt ccggcatcgc
      481 ggccaacccg gcggccgtcg ccgcgcccca gtcgtggcct acgaccacat cgcgtccggt
      541 cgggccggcc gcatccagca cctgcagcgc gtcgttcatc agcgtgccga cgtgatagct
      601 gccgtccgca ggaatcgacg acggcgcgta gccccgcatg aacggagcca ccacgcgcca
      661 gcctgcgtcg acaagtgccg gtgcgacctt ccgccaaccg tgggcggtgt cgggaaatcc
      721 gtgcaggcac aacgcaatcg ggtcatccgg gcgcccccag gtcagggcgc gtaactgcac
      781 cgatggcgtg gcgacatcaa gccagtccgg ctcgctcacg atcgctgatt cgttagacgg
      841 ggtcgaattt cgagatcagc cagcggtcgc cgaccctgtc caaggtgatg cgaacgctag
      901 aggcggtgtc ggtaggcacg ccattaccga cactcacggt ttgattgacg aagacaagga
      961 ccaccgcacg gccgggattg gcggataccg aggccgccgc gggcaccttg gcgaccgccg
     1021 aaatctgctt ctctttcgct cccggaataa cgacgtcgtt ggtcagtgac gtgtaggagt
     1081 cccggaaatc gccggtcagc agatcacgag ccgcaacaag ctgcttgtcg acggtgtcgg
     1141 gcttgtacga cagcagagcc acagtgctgt ccttggccgc ctgcaccgac tcgtcgcggg
     1201 cgacgtcgct tccgcgtacc gcagagtcct ggaacttcag gaacgcggcg cccaacgcca
     1261 gcagcagggc cagggcagga agcacggcga aggcgatcac tcgtcgcgag tccaaccgcc
     1321 gcctcttcgc gggttcggcc acgtccgcgg cttcggtcgc atccgcgggt tcgtcggtgg
     1381 catctgtcag cgtgttgcta tccgacttct catccgactc gctgggcgtc gcctcctcgg
     1441 tcggctcttc atcgaccctc acggtggtct cttcctccgc ggtcttgtct ttcggggtat
     1501 cggtcacggc acaaactcca cattgctcac cttggcttgg tcacccatct tctgcaccgt
     1561 gatacgcatc cgccatgagc gcggctcctg ctcggccgca ccggcattcg atgtcttgac
     1621 cgtcaccgcg accagtacct gagcctcgtc gcccgattcg gactccacgc ccgcctcggt
     1681 gacgctgccg accgacttgg acttcgcctg cttgacgact tggacgaacg gcgccgaacg
     1741 gttctggaaa tcgtcgtaga aggagccggt cgccgagtcg aggatgcgct gcacgtcgga
     1801 gtcggcgtgt tcccagtcga tcgtggtgag gttcagcgcg ccctgcctgc ccacctggag
     1861 aaacagattg cgctcgtcgg cggccttgcg cgactcgtag gcgcgaaaac ccagccaacc
     1921 ggtcagcgcc gccagcgcca ccaccgccac cagcccgagg atggtagcca gccgaacggg
     1981 cgacatcctg gggcggccca ccttgctttc gacgtcgtcg gcgtcgtctg cgtcgaggtg
     2041 ctctgtctcc tctgcctcga ctgccgtgga ctgcgcctcc gcgtcggtcg tcgtgccgtc
     2101 ggccgtctca gtgacagccg tctcggcgtc tgtcggttcc ttcgcacctg cgtcggggtc
     2161 gacgccgtgt ttagccatgc cttgctcctc cgcggctctg cgagccaatc ggcctgtgta
     2221 tacaccagcg cattcgatcc aacgtacgtg gcccggcttg gacgtagccg aagggcactg
     2281 tcacctggcc ggcccgcgcc ggtattctga tcaaaccgat aaatagttta cggtttgatt
     2341 ttcaccagcc gggaggagcc cacgtgcgcg tcatcgtgac gggtggaaac agcggcgtcg
     2401 gcaaggcaac agcaaccgcc atggccgctg cgggccacga cgtggtgatc gcgtgccgca
     2461 cgccgagcaa ggcacaccag gccgccgcat cgatcgccgg aaacgtcgag gtcgcggagt
     2521 tggacctggc cgacctggcg agcgttcgca agttcgcgga ttcggtggac ttcgtcgacg
     2581 ttctggtcaa caacgccgga gtcctcgggc tgccgctgac caggacggcc gacggcttcg
     2641 aggcccacat gggcaccaat caccttggcc atttcgcgct gacgtgcctg ctcggggatc
     2701 gggtacgcga tcgagtcgtc gcggtcgcca gcagcaacca cgcgttggcg aagatgcact
     2761 tcgacgacat caactggcac cggcgtcgct acaacccgtg gtccgcctac ggggagtcga
     2821 agctggcgaa tgtgctcttc gtgcaggaac tggcgcgccg taaccggacc gcttacgcgt
     2881 ccgatcccgg aatgacagcc accgacatca cccgcgacgg gtccggactg ctgcagtggg
     2941 ccggccggac gttgtcgcct cacatcgccc agagtcccgc cgacggcgcc cgctccacca
     3001 ttgaggcggt gaccaccgat ctgcccaatg ggacgtacat cgcaccccgg gggcttttcc
     3061 accagtgggg caagccgaag tcgacaaagc tcgccgccaa ggcgcgcgat ccggagagcg
     3121 cccgcagatt gtgggatctt tccgccgaac tgaccggctg tgattggcag ggggccggcg
     3181 catgacgaca gatcacacgt accgcagacc cccgacccgg ctggcgccca ctccgacacc
     3241 cgagtcgatg gcgttctgga ccggcgggcg caatggcgag ctgctgatca atcgctgcca
     3301 cggttgcggg cgtttcttcc atccgccggg gccggcgtgc tggcgctgcc gaagcaccga
     3361 cgtcgcaccg gagccggtgt cgggcagggg caccgtcgcc gcctacacca tcaaccggca
     3421 gccatggata cccgggctcg accccccgta catcgtggcc atggtcgagc tcgccgacga
     3481 gcccgacgtc cggctcatca ccaacgtcgt cggggtttcg ggggatgaca ttcgggtcgg
     3541 cttgccggtc gaagtcttct tcgaagactg gacggcgatg tcgggcgaag aggacagcag
     3601 ggtatggctc ccgttgttcc gtccttcgcc tgactgatcg acgccatccg tcttcgtact
     3661 tccggattgg ctgcggcgcc ggtgttactg tgacagtcga ctgacatatt cagcgcaagt
     3721 ggacagggaa gagcatgacc ggatttgccg cgaacgtgac gctcaactcc cccgctgatc
     3781 tgactcccga gatactgaca tctctgctgc ggcgacatca ccctgatgtc accgttaccg
     3841 atgtccgact ccgcaagtcc tggcagggaa ccacctctca cttacacctc gacgtcgatt
     3901 acgccgaacc cgaaagtgtg ctgccccaac aactgtttat gaaaacgcag cttggcaccg
     3961 tgcacgacct gcccacagcc gtagacgaat cgttgtccga gggcggcggc ggcacggtgc
     4021 tcctggatga cgagacgacg ttctaccgag acctgcgcga cgatctcgac gtcgaaacca
     4081 tgaccacgta tttcgccgag cacctcgacg gtccgtcgca gtttgtgatt atcgctgagg
     4141 acatcacgtt gcgcggcgca gaagtgcccg acgcagtcgc cggcttgtcc gtcgaacaag
     4201 tcgaccagtt gttggcgacc ctgagccgga cgcacgcccc gttctgggcc agtccacgct
     4261 tgaaggaggg cggtgacttg tcgtggttgc agaaccccgt cacgggtcga ttcgccgact
     4321 tcttgcgcac cagcggattc gccatcattc gcgcgtttct ggaactgccc tataaacgag
     4381 aactcctgga gtccgcgggc accgatatcg acgcgatgga agcggcgttc tgggcgctgc
     4441 aggaccgggt ggccgtcgag cccatcacgt tgcttcacgg cgatcctcac ccccgaaaca
     4501 catacgtgct gcccgacggt tcggtcggac tgctggactg gcaactggtc cggcggggtt
     4561 catgggtgca cgatgtgggt tacgcgttga tcgcagcatt gccgcccgcc ttgcgtcgcg
     4621 accacgaacg agaattgctc gacaactacc gcagacgact gatcgacagc ggtgtcgacg
     4681 cgccgccgga ccgcgagcag atgtggaccg cctaccggcg gagcccggcg tggggtttct
     4741 gcatgtgggc catcacgccc gaccagatgt actccgtcga gattgtgcgc gcggtgctcg
     4801 gccgcttcgc cgaggcatac tcggatctcg gcacgggcgc gctgctcact tcgtagcctc
     4861 ccagaggagt tcaccgccgc cttcattgca cggatgaggc cgtcatcgcc gatcgctcga
     4921 gataggcgtg acccgactcg cccgcccact gcagctccgc ccagctgacc gtgtaggccc
     4981 gcaccgggtc cgtgactccg tagggcagac cgtccgacat ggacatccac atcgacgacc
     5041 gtacggcgca tgcgatctcg tgttccccgc ccagctcgaa ggaaaaggtc tcgccgtggc
     5101 gcgcatgtac cgcaggcggc ggtactgatg tgatgtcgac gtgccgcaga acgccgtcat
     5161 cgaccaggta tgccccctcg agtgtggtgt gtccgtcgcg agcccagtac cgacagagcc
     5221 cgaatgcgcg tccactgggc agcactgcgc cggcgatcac gtgtccgccc cagtccttga
     5281 gggtgtcggc accgcgaggt ccccgcgaat gatcgcgcca gccaacaccg ttgaagtcga
     5341 tgctcacccc gtcgacgacg agttggcccg tcacccgggt gagttgttcg taatgctcac
     5401 gggcccaact ctgctcggcc atcccctcgc ctgcggtcgc cgcgtcccac acgggggtta
     5461 cgcactcgac gttgaggtcc atgcgtacgt gactcttgga cccgtcggcg agcggggcgg
     5521 tccgcatttg ctcgtaggac gagcgtaagc agaagccgtc gaacgttatg cgccacttgc
     5581 gaaacggttc ctcgcagtga aactcgagat tcgcacccgc cggccggcgc tctgggatcg
     5641 tgcggtgata ggcccacatg ctcagtacgc cctgatcctg cgggagcgcg atgagaaccc
     5701 ggtcctccca catctcccac ttgtccgcga cggtgcccag gtgcagccac atgccgacgt
     5761 cattgacggg atcgtacgga gtgaacaaca ggttctcggt ccatgcggga atgtcggggc
     5821 actcctgcac cgttcgttca ctgtcggcac tcaacccgga ggacaactgc ccgatagtgt
     5881 tgctcatcga cgattccttt tatccggtcg aacgggccag tacggcgacc tcgccggtcg
     5941 aaccatcgac acgcacgacg tcgccggtgc gcagcacctt ggtgccgttg ccggtcccga
     6001 cgacgcagct gatgccgagt tcccgcgcca cgatcgcgcc gtggctggca ggactgccga
     6061 tgtcggtgac cagcgctcca gcgagcgaca ccagcgccgt ccatccggga tcaacgaact
     6121 tgcacaccag cacctcgccg tcgtcgagaa catcgtcgcc gtcggcatcg agcacgaccc
     6181 ggaccgtacc ctcgacgatg ccgggcccag cgggcattcc gcgcacgccc tcatcgtccg
     6241 tacctgcggt gtcgttcacc tcgatgggta cgggcatgcc cacgaaggac actggaatat
     6301 ccagtgcccg atactcttca cgccgctgcc tgcggaacgc gacgagttcg cgcgcgttgt
     6361 acggcagggg gcccagcagc tccgcggtag tgaggtagaa ggtgtcgtcg acggaatcga
     6421 gcttgcctgc cctgaccagc tctcggccga gcgcgcccgc ggccgcccgg gtgccgtcga
     6481 tcgcggtgag aaacgcggcc ttggtgagct ccattgaccg gacctgaaat ccggtgaact
     6541 gcagcagccc acgaagcgca acccggcgaa gcctggacaa cccggccagc agctcggatt
     6601 ccgcctgctt tcgagccgcc accgcgtgct tcgcacgagc ctggggtcgt tcagcctcgg
     6661 gccggtccgc atgtaccgca gcgatccgct cgactgcctc gggatcctcc cgccacgaat
     6721 gtccgatgac gttgccctca ttgggaccgt gaaatccatg ccgcgcgacg aactccgcca
     6781 ccgagagtcg cccctgcccc acaatccaca cgtcatcggc aaggccggtc tcgatgacac
     6841 cgccgtaccc ggcgagcaaa gtcggcacca gatcggcgtg tccgagtcgc ccagcgatcg
     6901 attcgatttg agcgcgaagg gtattcagga tcatccgcgt gcgaatgtgg acccgcatga
     6961 cctcggtgaa acggcggacg gagtcggcca gcagggtgct gggatccacc ggatcgcctg
     7021 gcagcacctc gctacgccac cagtccatct gatcgtcgta agcacgctgg ggcgcccagc
     7081 gctgccgtat cgcggccagc ggcgccttca ccgccacgaa cgggattcgg agcggatcgc
     7141 tcttggttgg ggtggcatcg gcgcgaaccg agcccaacag gtcgcgctcg aagtcgtcgc
     7201 cggtggtgcc gggtagcagc ccggccagct cgcgggcgcg gtcgacgttc atcgcctgcc
     7261 tgccgtagaa gcacgccgtc gcccactgat tcggatcatc gggaacagcc agcgcggacc
     7321 gagacagtac cccgaagtcg tagatgccgg cgcgactggc acattcgacc ccatcacccc
     7381 aaagcgacca gcacagtggc gacaggatgt ccggggtggc ctcgccgacg ttggtcaacg
     7441 tccacatccg gtccggctcg ctgcgccccc gaatagggtc cgtcaagccc gcgggcgttc
     7501 catcgtcggc cattgtcctc ctgcaccgcg ggtgacagtt cactgtcact ctcgatcccg
     7561 attctgacat gtctcgccaa gcctcgcgac gcttctggtc gaccgcccga tcggttctcg
     7621 gttgtgacag ttgcctgtca tactggccgg catgaggttc accgtcaccc acggttcggc
     7681 caaccgcggc ggcagttcga ccggactgga cgtggaccca acctcagttg cactgctcga
     7741 cggcagctgc ccgttgtccc gacaagagct gggcaacaag gcccacggca tcaacctgat
     7801 tcgacggctc ggcctgcccg tgccccctgc gttctgcctc acgacggctg tgtgcaccgc
     7861 gttcctgagc ggtgaccagc gcgcgctgga ccgcgtctgg cccgacgtgt tggagagcct
     7921 ccggtggctg gagcgcgaga catcgcgcac cttcggccgc ggcccgcggc cgctactgct
     7981 cagcgtgcgt tcaggcgcgg cacagtcgat gcccggcatg ctcgacaccg tgctcgacct
     8041 cggcatggac gacgatgtgc aagacgcgat ggcctcaagt gccacaccag ggttcgcgtc
     8101 tgacacccgc gcccggttcg actccatgta ccgccggatc gtgctcggtt ccgacgagtc
     8161 ggcggatatt ccagacgacc cgttcctcca actccgcggc gcaatcgaag cggtgttcgc
     8221 ctcctggaac agtccccgcg ccacggcata ccgtgcccgg cacgggctcg acggtaacgc
     8281 aggaaccgcg gtcgtgatcc aagcgatggt gttcgggaac ctcggtgggc actccggaac
     8341 cggcgttctg ttcagccgca atccggtgac gggcgccgac gagccactgg gggaatggct
     8401 gccttgcgcg cagggtgagg acgtcgtctc gggaacctac gattgcctgc cgctgaacgc
     8461 attgcgggac gcgcaaccag atgtgtacgg cgagctcctg actgcggccg ccgagctcga
     8521 acgcttcggc cgcgacgtcc aggacgtcga gttcaccgtc gaagaaggtc gactgtggct
     8581 gttgcagacc cgcgtcgcga agcgctctcc ccaggcggcc gtccggctcg ccttgaagtt
     8641 ccgcagttca ggcgtcatcg atgacctcga gaccgcgcaa agggtaaccc cagatcatgt
     8701 ccgcgcgctg ttgtcgccgt cgctacaacc cgacacctgc agcgccgcca cgctgttggc
     8761 aaaggggctg cctgccagcc ccggcgtcgc tttcggcacg gcgcacaccg acatcgacga
     8821 tgcgatcgac gccgcagacg cgggtgagga tgtgatcctc gtccgcatct cgaccagccc
     8881 cgacgacgtc gcgggaatgc tcgcggccaa ggccgtcgtg accgaaatcg ggggcgccac
     8941 atcccacgct gcggtcgtca gtcgcgaact gggcctgccc gctgtggtgg gttgcggcgc
     9001 tggggtggcc gcggcgctcg agggccgatc ggtcactgtc gacggcggcg cgggtgaggt
     9061 ctacgacgga gcgctggaag tcgcggcatg gtcggaacga gacagcccgg aactgaccga
     9121 gctgaccgcg atcgccctgc gggtgtcccc gctacgggtg cacaccgccg gtgcgcatcc
     9181 aaagctttcg gcgacaaccg aagccgcggt gcatgaggcg gtcagtgctg ggcgcacaga
     9241 cgtcgtcagt gacgctcccc tgatcgcgat gctcacggca gtcgcactcg cagggaggtc
     9301 tgaggatgga tgagctggcg ctactgcagg ccatccggct caaaggccgg gtgaggcccg
     9361 cgggcctggc cgcgactctc cagcaggacg aggacgtcgt cgccaccgcg gttgaggatt
     9421 tggcggaagc cgggctggtg gtgcggggca cggcggtgcg gctgacgccg gagggcaagg
     9481 accggttggc cgaatttctc gcagaggagc gcaacgggat cgacggccac gcgatcacgc
     9541 gggtctgcga cgactttcgc accgtgaacc gggaattcaa ggcgttggtc tccgactggc
     9601 agctccgcaa cggcgggccg aacgaccatt cggacgcgga ctatgacgca gcggtactgg
     9661 gcaggctcga aggcgtacac ggcgcgatcc tgccgattct ggcctgcgcc tctgagcagc
     9721 ttccgcggct gaacacgtac tcggcgaagc tgtcgacggc cttcgtgaag atccagggcg
     9781 gcgaccccac gtggttgacc cggccgctcg tcgactctta ccacacggtg tggttcgagc
     9841 tacacgagga actgatcggc gcggccggac tgacccgtga ggacgaggcg ggcgacggcc
     9901 acgcgggata gcgcgactca acacgaagcg tcgctggata tcgccaacct cacccgccag
     9961 tgatcagctg gaaacctacg tcaggcgata ttcataccgg catcgaccat caacgtgctg
    10021 ccggtgtagt accggccgct gtcgttggcg gcgaggaaca acaccgtctc gctgacgtct
    10081 cccggctcga tgagctgaac gggcagggag ttcttgaagg cgttcgccag gtgttcgtgc
    10141 ttggcgatca cttcaaacaa tgccgggttg tcaacgatca tcgtcgtcgc aaccccggtc
    10201 ggggcgatcg cgtttacgcg aatattgttg ggcgccaacc tctgtgcgta ggcccgaacc
    10261 aggccggtga cgccgatctt ggcggcggca taggcatcgg atccgccggt gccgtcggtg
    10321 agcgcccgca gccccgccat tgagctggtc acgatcatcg ctccaccgcg gcccgcctcg
    10381 atcatgtgct cggtggcgat ctgcatggtg ttccacacac ccgtgagcat cacgccgatg
    10441 ccgagctccc acgccgccac cggatcgggc tcgtcgacac gattcagcag aatgcccgcg
    10501 ttggcgacga cggtgtcgat gtggccgaat tcggcgacac cgtcggcgaa cgcggcggcc
    10561 aacgcgtccc ggtcacggac gtcggcctgt cttgcgacca tccgccttcc ggtcttctcg
    10621 accaggttca ccgtctcgtc gaggtcctcg ggggatgcca gcgaatacag cacgccgtcg
    10681 agttgcctgc agatgtcgat tccgacgatg tcggcaccct cctccgccag cctgaccgcg
    10741 tgcgagcgtc cctgtccccg tgcgactccc gtgatgaagg cgacgcgacc atccattctt
    10801 cccacgtgcg actcctaagc actttccttg actacgaact cggggattcg gaaaccgctc
    10861 tcccccagat ccgcgatgcg cagtttcacc ggctggccga tacggacatc cgcgggatcg
    10921 agatcgagga ccgcactgat ccgcagtccc ggctgctcgg ccaattcgac cagcgcgatg
    10981 acgtacggcg gcgtgcggcc cggaaccagc gcctggcgaa tcacgatgta gctgaagaca
    11041 gtgccctcgc cgccgacctc ctcgaagtgc acggggcctc cggtgaatcg gctgcgctcc
    11101 agcggcgggt ggatccattt accggtgtcg tcgcagcggc acagcatcaa ccttccgtcc
    11161 ttcagaccgt cccagaacgg cgccgtgtcg ggatcgggaa ccggaatcgg cggcagcagt
    11221 tgcggattgg tcatggcttc tccctgctat agacgttggc gccaccgaat ggacccgcac
    11281 ctgcggtgac aagtgccagt tgcgcgtttt cgacttggcg aacacccgcc gcgtggcgca
    11341 gttgcagcgc cgcttcaaag tggtggttca gcccgtggat gtagccctcg gacagcaatc
    11401 caccgtgcgg attgaccacg acgtcgccgc cgtaggtggc acgacccgtc gagaagaact
    11461 tcccgacttc gcccttctcg cagaagccga acccctccat cgtcgccatg accgtgtagg
    11521 tgaagcagtc gtacatgcat gccacgtcca tgtcgctggg cgtcagcccg gtgttcgccc
    11581 agatcttcgg tccggccgtg cgcgagaaca tctcggtcgg gtcgtcccac tcgctccagc
    11641 ctgagccgcc ctgggagttc gacgatccga ccagccacat cggcggctgc ttgcagtcac
    11701 gggccatgtc ctcacccgtg atcaggatcg ccaccgcacc atccacttcc gacgtgcagt
    11761 ccaagacccg gaacggttcg ttgatccagc gcgccgcgag atattcgtcc atacccagcg
    11821 gttcgcggcg cagggcgtgc tcgttgggtc ccgcatgcgc tcgttgtgtg attgcgatct
    11881 gtcccatgtc ttcgcacgtc gacccatact cgtgctggtg ccgacgcgcc cacatggcga
    11941 tccactgagg cggcaccatg aaaccggacg cggcgtcgaa ttggtcgtgg ttcggcacct
    12001 gcatcggccc ctcgacgtgg ccgaaccggt atccggagcg gccattgagg gaccggtaca
    12061 ccagaaccgc cttgcacatg cctgcttcga tgacggcggc cgccgttgcg atctggtcgg
    12121 cgaccaggtt tccgccgccg tacatcgcgt tggcccaagc caactcgtcg atgccgagcg
    12181 cccatccgac attgatgggc tgctcggagt cgttggccat atacgtacaa atgccgtcga
    12241 tgtcggcgga cgtcagtccg gcatcggcga tcgcatcgcg gcacgcggac acggccatcg
    12301 cacgtgtcgt gcgccctgaa ctacgtgagt aggtggttcg accgattccg actactgcac
    12361 aggtcataag actctccgat ctacttcggc gcgggctctc ggggcaggcc gaggattcgt
    12421 tcggcgatca gattcctgac gacttccgac gtccctcccg cgatggtgag gcatcgactg
    12481 aacagatagt cgtgcaagac attaccttcg tctccgagca tcagcgccgg tcctgcgatg
    12541 cggagcgcaa gctctgcgac gcgttgggcg tgttcggcgc cgaacagctt ggcgatgttg
    12601 ccctcgatcc ccgggccggt gtcgaaaacc gcacgggccg cctgccgaag gttcagcgtg
    12661 gcggcggcat acgcctcgat aagcagtgcg ccgacatcgc gcaccaggcc gccgtcgtcg
    12721 ggccggtggc gcgccagcaa ctcgagcaat gcatcggcgg tcatcgtcac cgatccgccg
    12781 ccgatcgaca cccgctcatt gccgagcgtc gccatcgcga cggtccagcc ggcgttcacg
    12841 gcgcccacga cgtcgcggtc gggtacgaag acgtcgtcga agaacacctc gttgaacaat
    12901 gtttcgccgg tgatctcctt gagcggccgg atccggacat ccggatcggc gaggtcgatg
    12961 accatcgcgg tgatgccctt gtgtttcggc acattcggat cggttcgcac ggtcgccagg
    13021 ccacgctgac agttcatcgc gtcgctggtc cataccttct gcccggtgac cgaccagccg
    13081 ccgtcgacct tcttggcctt ggtggcgacc gcggccgcgt cagagcccgc gcccggctca
    13141 ctgaacaact ggcaccaccg cagctcgcct tcgagactgg gccacatcaa gcgctcgatc
    13201 agctcgtcgc tgccgtgctg cagcagcgtg ggcaccaccc atccgcccag accaaggctc
    13261 ggcttctcaa gcccgtcgag cgcttcctcg atgatcaact gctcgaccga gtcggcgtcc
    13321 cgaccgtacg gctcgggcca atggggttga agataaccgc tgcgtgccca gatcttttgc
    13381 tgatcagcag cgtcggcgcg ggacagctcg gcacggaatt cctctgcggc cctgcgatac
    13441 tgctcggcct ccgggggcag gtcgaccgag tgacgccgac gaagtccgcc tgactgcagt
    13501 tcggctacct tgtcgcgcaa cgtctcctgg ccaccgacga aggcgagcaa caccgtcgcc
    13561 cgccggatgt agaggtgcgc gtcgtgttcc catgtgtagc cgatgccgcc gtgcacctgg
    13621 acattctgca gcgccactcg ttgataggcc ggcagcacgt gtccggctgc catcgttgcg
    13681 gccagttcgg cctcttctcc gtgggcccgc gcggcatccc acgtgaccgc cactgcgagt
    13741 tcagtgtcga gcagcatgtt cgcgcagtgg tgcttgatcg cttggaacga cccgatcgga
    13801 cgtccgaact gttcgcgtgt cgcggcatag gccgtcgcca tctcggtgca tgcggcgaga
    13861 ccgccgaccg cctccgaggc agccaggatt ctgagaacgc gggcagcgac tccggcggca
    13921 ccggggaaaa cgtcggccac cggagccgac gagaggccca acacggtgat cggtcgcatg
    13981 atctggtcca ccgagtcgac acttcgggtg gtgacggcgc tgcccgcctc gacgagcacc
    14041 agatcctccc cgaccggcac caggaagacg tctgcggcgg attccgccag cgcggcgacg
    14101 gaatcggcat tgacggtcga atccgactgg gccgcatcgc ttgtcgtgcc aatccccgcc
    14161 acgacgtcgc ccgatgtcag acccggtaac catcgcttgc gctgctcatc ggtgccgacc
    14221 tcggcgatca ccgacgacac tgcgaccgtc ggcagaaacg ggccgccgat ggccgcacga
    14281 ccgagttgct cgagcactat cgtcaactcg ggcagcccgt acccctcgcc accgaactgt
    14341 gcgtcgacgt gcatgccgag ccagccggcg gacacgatct ccttccacag gccgtcgtca
    14401 tgccaccagc ggcccttcga ctcgggctcg agcaggatgc ggcgggctcc cgccgtgccg
    14461 gagcggccgg ccaccatcgc tttcgcgacc tcggcgagtg ctcggtggtc ttcggttatc
    14521 gctaacgaca tcggcaacgc tccgttcgtg ttgagactct caccaggggg tgccgaactg
    14581 ttcgcgatac gtcaccgaga ccgccggacg acgacggggc ggcgagagct tcctggccgg
    14641 cttgccgagg tagaccgcgg ccatcggttg caacgactcg ggcaattgga gcttttcgcg
    14701 gacccggtcg acaccgaagg tggtcagccc ggtcgtcagg caggacccgt agcccaggtc
    14761 ggccgcggcg agcaggagat tctgcaccgc cggatagatc gaggacggcg cgtagatctc
    14821 cggcacccgt tcggtgtcgg cgcataccac gacgaccacc ggcgccgcgg cgaatccgcc
    14881 tcgggtgaaa ccgtactcga ggtcttcgac caacaccttg tcatcgacgc tctgccggac
    14941 gaagtcgcct ccgcctgcgt tccatgtctc ggtccaccat tcggccaact gcgccctggt
    15001 gtcctcgttt cgcaccacga cgaacgacca cggttgggtg ttctcggcgc tgggggcgtg
    15061 gacggcggac tccaggatgc gctcgaggtc ggcgtcgtca accttgccgt cagggtcgaa
    15121 tcgccggcac gcgcgctgcg cgtgcatgac ggacagcagc tcgcccacta cgagcccgcc
    15181 accacacggt cgccgcgtgg gacccatccc gtcaacgaga tcgtgctcaa atccagccac
    15241 tcgcccagcg acagcgtgcc cgccatcacg cgcttgagga attcggacat caccacatcg
    15301 gggtcgttgt ccagttcgta gatgacgaga tagcggtgag tcggcggcgg caactgcgcg
    15361 ggcaactcct catcgtcggg gacgttcact tcggcgatgt cgtagcgctg tgcggcgacc
    15421 acaccgggca catcgaggac ctccggcaca tgctgcgcgt cataccactt gttgaacgcg
    15481 tcgtcttcgc cctcgatggg gttggtcagc acaacgaaga tgtttggggc catgggaaac
    15541 tcgcctttca cgtcggccgg tgtcatcact gacactatga cagtcgactg tcacagtcaa
    15601 cgccgccggt cagctcattg actgtggcag tcgactgtca tagtcttcgg cccagtattc
    15661 cccggccatt ccggaaggca catcatgcga ttcgctgtat acctacccaa ctgcatgcat
    15721 gttggagcga tcacccagcc gtgggagcac ggcctgaccg gcccagacgt cgcactgacc
    15781 gctcagaccg ccgaacgcct cggatactcg atggtgtttc tgcccgagca cttcttgacg
    15841 cccaccagcc acgtcgaact ctccggcgac cactatttcg acgccaccac cgcacaagcc
    15901 ttcgtcgccg gcgccacctc gtcgatcacc atcggttcga tggtgacgat cctgccgctc
    15961 cacaatccga tcatcgccgc caagacaatt tccacactcg actggctcag cgatggacga
    16021 gctcaggtca ccgtcggggt gggttggctc aaggaggagt atgacgcgat cggtgtcccc
    16081 ttctccaagc gcggccgcat cgccgacgag tcattggccg ccatgttcga gctgtggcac
    16141 agtgacacac cgagttttga cggcgagttc gtgaagttcg atgacatcgc attcggcccc
    16201 aagccgatcc gcgaaccaca tcccgtcatc tggatgggcg gcgacgccga cgcggtgctg
    16261 cg
//
LOCUS       DASYLX010000844         6484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3010395,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000844 DASYLX010000000
VERSION     DASYLX010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010395"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGP27_19830"
     CDS             complement(<1..300)
                     /locus_tag="VGP27_19830"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7853583.1"
                     /translation="MAAPQVPNAYADFVNVLVLVGLPANPVNRAVSRLAIDSSPEGVV
                     VNVFGDLMCLPRYSEALESRGTPAAVVALRTAAIEADAVLVVTSYRGRIPSVAHNA"
     gene            complement(400..996)
                     /locus_tag="VGP27_19835"
     CDS             complement(400..996)
                     /locus_tag="VGP27_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853584.1"
                     /translation="MESVEYVLRWRMLRACGRNPLVRGIDRVELLIVALGTLVALVSV
                     AYAGALGTAVHDARSRLYIAQAQTRHTLTATATKDSAVVPRADDSTATRVTARWQVDG
                     TEHVGTFNWDHAVKLGDPLTIWVDRNGDRVDPPTPTSQAAEDAVGVAFVAWQTVVLAT
                     ACLVWLGRSYLDRRRDSAWERDIRCLIDGSGGRTNRKP"
     gene            complement(1024..1698)
                     /locus_tag="VGP27_19840"
     CDS             complement(1024..1698)
                     /locus_tag="VGP27_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV7853585.1"
                     /translation="MSETRQDTSTPAGTVGRFFADAVSKTRFWWLLLITGAAWIVLSI
                     VILRFNYTTVAAVAVLFGVYCLVAAVNEVTIGAVSSSTGWRIAHGLLAALLVVVGVVS
                     LANLGATFVTLAAIISFYFIFRGCFDIAMAFAGSAVPGWWVLLISGLIELGLGFWAAG
                     SWNVSVVVLVAWVAAGALVHGIGEIALAFQLHQGRSGITSMEGLADRLGVTNNHPAPD
                     AAVPVR"
     gene            complement(2032..2226)
                     /locus_tag="VGP27_19845"
     CDS             complement(2032..2226)
                     /locus_tag="VGP27_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853586.1"
                     /translation="MTEPVERIRQILERHQSYGESGHARHVAEDIVAQLGLQREIAHD
                     KMRYASAWFDGELTKVEGAE"
     gene            complement(2245..3429)
                     /locus_tag="VGP27_19850"
     CDS             complement(2245..3429)
                     /locus_tag="VGP27_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853587.1"
                     /translation="MITATITDSADFYGLESLLDDDERDFLHRVRDFMQSEVAPSINQ
                     HWQRGSFPFEIVPGFRQLGLAGLPYQGFECPGKSFLLDGMVAMELARTDSSIATFAGV
                     HGGLSMGSIYLCGSEDQKQRYLPGMARFDKIGAFGLTEPDVGSGASGGLTTTARRDGD
                     SWILNGQKKWIGNATFGDLTVIWAKDVTDGQVKGFVVANDSAGFSTTKLEDKIALRIV
                     QNALITLDDVRVSEDDRLANANSFRDTAEVLRMTRAGVAWMAVGCARGAYENALAYAI
                     SREQFGRPIAGFQLVQDLLVRMLGNITSSWALCARLSQLQDEGHAEDRHSSLAKAWCT
                     VRMRETVGYAREILGGNGILLEHNVGRFVADAEAIYSYEGTREMNTLIVGRAITGLSA
                     FV"
     gene            complement(3441..4271)
                     /gene="phaZ"
                     /locus_tag="VGP27_19855"
     CDS             complement(3441..4271)
                     /gene="phaZ"
                     /locus_tag="VGP27_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEV7853588.1"
                     /translation="MTTAAAAAGLSVERLIPVLGQQIRVNVRTGRGVPLVLCNGIGAG
                     LEVLDPLVERLDPDTTVVRFDVPGSGGSPNSRLPYAFPYLAAALGRLLTELGFDGRVD
                     VLGFSWGGALAQQFAFQNPRRCRRLILVSTGTGALMVPGGPAVLAKMLTPRRFLDHDY
                     ASVIAGALYGGTVRTDPTLVKQLFDRQLMAGSRVGYLHQLLAGAMWTSLFALPLIRQQ
                     TLIVAGLDDPIIPVVNAKVMARLLPHATLHLHAGGHVDMITNAAELAPVIESFRHQPD
                     "
     gene            complement(4268..4720)
                     /locus_tag="VGP27_19860"
     CDS             complement(4268..4720)
                     /locus_tag="VGP27_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7853589.1"
                     /translation="MTTVLEEPADLLKLVGQRLGTTAWMKVTQRQVDQFAEATGDRQW
                     IHTDPARAAKGPFRGTIAHGYLTLSLAAAVISQVLEIREQTAALNYGLNRVRFPSPVR
                     VGTRIRAAVTVMSAQQKTSGVESVFTLTYEIDGEDCPACVADVIVLYP"
     gene            complement(4738..6432)
                     /locus_tag="VGP27_19865"
     CDS             complement(4738..6432)
                     /locus_tag="VGP27_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853590.1"
                     /translation="MAVTTRAGANELAAPLDLLLTSSAVGMADRLVPNVAWSRFALNL
                     AKQPRTVASRAASLGRELMSIAEGRSELAPAKGDKRFADPAWRGNPLLHRTMQAYLAT
                     NSTVDQLFSDANLDWRDAERIRFALDFLTEGLAPSNNPILSPLGWKALIDTGGLSAVR
                     GLRHFVDDMMSAPRVPSMVDPDAFSLGEDVAVTPGSVVFRCEEFELIQYAPQTEKVLS
                     APLLMVPPVINKFYIMDIASGRSMIEYFVRQGVQVFAISWRNPSAEDRNWGFDTYGQA
                     ILNALEAVEKITQTARTHLQASCSGGILAAMTAAHLNSSGEGQRLASLTLMVTVLDQR
                     KAGFASAALDEEAANIAVALSARKGYLDGRSLAEVFAWLRPTDLVWRYWVNNYVQGKS
                     PAPFDVLYWNADTTRMAAALHRDMVMTGLHNSLATPGAASMLGMPVDLSQLTTDAYVV
                     AGIADHISPWQACYRSARLLGIKDLRFVLSSSGHIASLVNPPGNPRASYRLGGVDEPD
                     PEFWLESAEQQTDSWWPDFVAWLGERSGEKKDAPAALGGAGMAPLEPAPGTYVLER"
BASE COUNT         1221 a         2152 c         1956 g         1155 t
ORIGIN      
        1 tgcgttatgc gccaccgagg ggattcggcc gcggtagctc gtgacaacga gcaccgcatc
       61 agcctcgatt gccgccgtac gcaatgccac gaccgcggcg ggcgtcccgc ggctttccaa
      121 cgcttcgctg taacggggca ggcacatgag gtcgccgaac acgttgacca ctacgccctc
      181 gggcgagctg tcgatcgcca gtcggctcac cgcacggttg accggattcg caggaagacc
      241 gaccaacacc aacacgttaa cgaagtccgc atatgcattt ggtacttgtg gagccgccat
      301 cagttgcaac cttgtctgct ctcgagatga aatcctctgg ggtctccggc gattgcgcac
      361 aaagcgaatt cggacgcgcc gatggttctg gccgggccgt catggtttac ggtttgtccg
      421 cccgccgctg ccgtcaatga ggcatcggat gtcgcgttcc caagcggagt cgcgccggcg
      481 gtcaaggtat gagcggccca gccagaccaa acaggccgtg gcaaggacca ctgtttgcca
      541 tgccacgaat gcgacaccga ccgcgtcctc cgcggcttgc gatgtcggcg tagggggatc
      601 gacccgatca ccgttgcgat ccacccaaat cgtcagcggg tcaccgagtt tgacagcgtg
      661 atcccaattg aaggtgccga cgtgttcggt gccatcgact tgccatcggg cggtcaccct
      721 cgtcgctgtg ctgtcgtcgg ctctgggaac gacggcgctg tccttcgtgg ctgtcgcggt
      781 caacgtgtgc cgcgtctgcg cctgcgcgat gtacagacgg ctacgcgcat cgtgtacggc
      841 cgtgcccagc gctcccgcat aggcgacgga gaccagagcg accaaggttc ccaatgcgac
      901 gatcaacagt tcgacgcggt cgatgccacg caccagcgga ttgcggccgc acgcgcgcag
      961 catccgccat cggaggacat attcgacgct ttccatgacc actccctcgc cagcgcaaat
     1021 cagtcaccgg acggggacgg ctgcgtcggg tgcggggtgg ttgttggtga ccccgaggcg
     1081 atcggccagt ccttccatgg aggtgatgcc gctgcgtcct tggtggagct ggaaagccag
     1141 ggcgatttcg ccgatgccgt gcacgagggc cccggcggca acccacgcca ccaagaccac
     1201 cacggagacg ttccatgacc cggccgccca gaaccccaac cccagctcga tgagcccgga
     1261 gatcaacagt acccaccatc cgggcacggc gctgcctgcg aaagccatgg cgatgtcgaa
     1321 gcaaccgcgg aagatgaagt agaagctgat gatggcggcc agcgtgacga aggtggcgcc
     1381 gaggttcgcc aaggacacca cacccaccac caccagcagc gccgccagca ggccgtgcgc
     1441 gatgcgccag ccggtggatg aggacactgc cccgatcgtg acctcattca ccgcggccac
     1501 caggcagtac accccgaaca gcacggccac cgccgcgaca gtggtgtagt tgaagcgcag
     1561 gatcacgatg gaaaggacga tccaagcggc gccggtgata agcagcagcc accagaagcg
     1621 ggtcttggac acggcgtcgg caaagaaacg acctactgtc cctgccggtg tcgacgtgtc
     1681 ctgacgagtc tcgctcattg gaactcctta gcattcggtt gatgtagcct cgccaacccc
     1741 tcaccggcga gaatgagtca agagtgcgct cggccgtaaa cgatttcgat gtgttgtgac
     1801 acaacatctc tgtcgcaatg cggtgcctgc caacaacgcc atccaggcct gcttccggct
     1861 cgcgcccaat ctgacgcttc aggttcgtcg aaagaaaacg acttattcgt gcctcccatc
     1921 gggtttaccg tcgcggttgc ggtaggagcc aaggtcttga aagcccatga accgcgcttg
     1981 acggtgtcga ttcaggtcga cttgcgccga acccgactcc tctgaggtcc atcattccgc
     2041 gccttcgact ttcgtcagct cgccgtcgaa ccacgcactg gcatatcgca ttttgtcgtg
     2101 tgcgatctcc cgttgcagtc ccagctgcgc gacgatgtcc tcggcgacat gacgcgcgtg
     2161 gcccgactca ccgtatgact gatggcgttc aaggatctgc cgaatccgct cgaccggctc
     2221 ggtcattcta tgtctccctt ccacttacac gaacgcgctt agcccggtga tggcacgccc
     2281 gacgatcagc gtgttcatct cgcgggtacc ctcgtaggag tagatggctt cggcgtcggc
     2341 gacgaagcgt ccgacgttgt gttccaacag aattccattg ccgcccagta tttcccgtgc
     2401 atatccaacg gtttcccgca ttcgcaccgt gcaccaagcc ttcgccagcg acgagtgccg
     2461 gtcctctgca tgtccctcgt cctgcaactg cgacagcctg gcgcacagcg cccacgacga
     2521 tgtgatgttt cccagcattc gcaccagcag gtcctgcacc aactggaaac cagcgatcgg
     2581 cctgccgaac tgttcacggg atatcgcgta cgccaaggcg ttctcatagg ccccgcgcgc
     2641 acagccgacg gccatccagg ccacacccgc gcgggtcatg cgcagcactt cggcggtgtc
     2701 acggaatgaa ttcgcgttcg ccagtcggtc atcctcagac accctgacat cgtcaagggt
     2761 gatgagggcg ttctgcacga tgcgcagcgc gatcttgtct tccagcttgg tggtagagaa
     2821 cccagcgctg tcgttggcca cgacgaaccc cttgacctgg ccgtcggtga cgtctttggc
     2881 ccagatcacc gtgaggtcgc cgaaggtggc gttgccgatc cacttcttct gcccgttgag
     2941 gatccacgag tcaccgtccc gtcgagccgt cgtcgtcagc ccacccgagg cgccggaacc
     3001 gacgtcgggt tcggtcagcc cgaacgctcc gatcttgtcg aatcgagcca tgcccggcag
     3061 gtatcgctgc ttctgatcct cgctaccgca caggtagata gaacccatcg acaagccgcc
     3121 gtgcacaccc gcgaacgtcg cgatcgacga gtcggtacgg gcgagttcca tcgccaccat
     3181 gccgtcgaga agaaacgact ttcctggaca ttcgaatccc tgatacggca gtccagccag
     3241 cccgagttgc cgaaagccgg gcacgatctc gaaggggaag ctcccccgct gccaatgctg
     3301 attgatgctc ggcgcgacct cggattgcat gaagtcgcgt acacggtgca agaagtcacg
     3361 ctcgtcgtcg tcgagcagcg actccaatcc gtagaaatcg gcgctgtcgg taatggtagc
     3421 ggtgatcatc gggtgctccc tcagtccggc tgatgtctga aactctcgat caccggcgcc
     3481 aattcagcgg cattggtgat catgtcgacg tggccaccgg catgcaggtg cagcgtcgca
     3541 tgcggtagca accgcgccat gaccttggcg ttcaccacag ggatgatcgg gtcgtcaagc
     3601 ccggcgacga tcaacgtctg ttgccggatc agcggcaagg cgaacaggct ggtccacatc
     3661 gctccggcaa gcaactgatg aaggtagccc actcgcgaac cggccatcag ctggcggtcg
     3721 aacaattgct tgacgagggt cggatcggtg cgcacagtgc cgccgtagag cgcgccggcg
     3781 atgacggaag cgtaatcgtg gtccaagaac cggcgcgggg tcagcatctt cgccagcacc
     3841 gccggccccc caggcaccat gagagcacct gtgccggtgg acaccaggat cagccgtcgg
     3901 cagcgacgcg ggttctgaaa cgcgaactgc tgagccagcg caccgcccca cgaaaaccca
     3961 aggacgtcca ccctgccgtc gaatccaagc tccgtcagca gccggccgag cgcggctgcc
     4021 aagtacggaa atgcataggg gagccgggaa ttcggcgatc cgccgctccc cgggacgtcg
     4081 aaacgcacca cggtcgtatc gggatccaaa cgctccacga gtggatcgag cacttccagg
     4141 ccggctccta tgccattaca caacaccagc ggcacgcctc tgcccgtgcg tacattcact
     4201 cggatctgct ggccgagcac cggtatcagc ctttccacgc tcagccccgc cgcagccgcc
     4261 gcggtggtca tgggtacaac acgatgacat cggcaacaca tgcagggcag tcttcgccgt
     4321 cgatctcgta tgtcagcgtg aacaccgact cgacacctga tgtcttctgc tgggcactca
     4381 tcaccgtgac tgccgcgcgg atccgggtgc ccacccttac cggcgacgga aagcgcactc
     4441 tgttgagccc gtagttcaac gcagccgtct gctcgcggat ctccaacacc tgcgagatga
     4501 cggcggcggc gagtgagagc gtgagatagc cgtgcgcgat ggtgcccctg aagggaccct
     4561 tcgccgcgcg cgctgggtcg gtatggatcc actgacggtc gccggtggcc tcggcgaact
     4621 ggtcgacttg ccgctgggta accttcatcc acgcggtcgt gccgagtcgc tgtccgacca
     4681 gcttgagcag atcggccggt tcttctaaaa cggttgtcat acagactccc tcgttggtca
     4741 gcgctcgagc acgtaggtgc ccggggccgg ctccagcggc gccatccccg cgccgccgag
     4801 cgccgccggt gcatccttct tctcgccgct gcgctcgccg agccatgcga cgaagtccgg
     4861 ccaccacgaa tccgtctgct gctcggcgga ctccagccaa aactcgggat ctggctcgtc
     4921 aacgccgccg agccgatagc tcgcccgcgg gttgcccggc ggattcacca gggaggcgat
     4981 gtgtccgctg gaggacagca cgaagcgcag gtccttgatc cccagcaggc gggcactgcg
     5041 gtagcacgct tgccacggcg atatatggtc ggcgatcccc gcgacaacat aagcgtccgt
     5101 cgtcaattgg ctgaggtcta ctggcatacc cagcatgctc gcggcgcccg gtgtggcgag
     5161 cgaattgtgc agacccgtca tcaccatgtc ccggtgcagc gcagcggcca tccgtgtggt
     5221 gtcggcgttc cagtacagga cgtcgaaggg ggcaggcgac tttccctgga cgtagttgtt
     5281 cacccaatag cgccacacca ggtctgtcgg ccgcagccac gcgaagacct ccgccagtga
     5341 ccggccgtcc agatagccct tacgcgcaga caaggcgacg gcgatgttgg cggcttcctc
     5401 gtcgagggcg gccgaggcga aaccggcctt cctctgatcg agaaccgtga ccatcaacgt
     5461 caggcttgcg aggcgttgcc cctccccgct cgagttcaga tgagccgcgg tcatcgcggc
     5521 gaggatccca cccgaacagg aagcctgcag atgggtgcgg gccgtctggg tgatcttctc
     5581 gacggcctcc agggcgttca ggatcgcctg gccatatgtg tcgaatcccc aattccggtc
     5641 ttccgcagag ggattccgcc aggagatagc aaacacctgt acgccctgcc ggacgaagta
     5701 ttcgatcatg ctgcggcccg acgcgatatc catgatgtag aacttattga tgaccggtgg
     5761 caccatcagc agcggcgccg acaagacttt ttcggtctgc ggggcatact ggatcagctc
     5821 gaattcctcg catcggaaga ccaccgagcc cggtgtgacg gcgacatcct cgcccaacga
     5881 aaacgcgtcg ggatccacca ttgacggaac ccgtggcgcc gacatcatgt catcgacgaa
     5941 gtgccgcagg cctcgcacgg cgctgagtcc gccggtgtcg atgagcgcct tccaccccag
     6001 cgggctcagg attgggttgt tgctcggtgc gagcccttcg gtgaggaaat ccaacgcgaa
     6061 tcggatacgc tcggcgtcgc gccagtcgag gtttgcgtcc gaaaaaagtt ggtccacagt
     6121 gctgttcgtc gccagatagg cctgcatcgt gcggtggagc agcggattgc cccgccatgc
     6181 cggatccgcg aatctcttgt cgcccttcgc aggggccagc tccgagcggc cctcggcaat
     6241 cgacatcaat tccctaccga gcgacgctgc gcgcgacgcc accgtgcggg gctgcttggc
     6301 gaggttgagt gcgaatcggc tccacgccac gttgggcacc agccgatccg ccatgccgac
     6361 ggccgaactc gtcaacaaca aatccagcgg cgccgcaagc tcattggcgc ctgccctcgt
     6421 ggtgactgcc acgtgtcacg cctcctcggg tggtggtttc acttcacggc gcaagagctt
     6481 gccc
//
LOCUS       DASYLX010000845         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2901779,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000845 DASYLX010000000
VERSION     DASYLX010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901779"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VGP27_19870"
     CDS             complement(<1..1542)
                     /locus_tag="VGP27_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7853591.1"
                     /translation="MFEGLAPTELVVAIADSQRQESRLMARRLSAVAELLALRTAEVY
                     TEDPDPGYMIVTGFQRTTAEVAAACNLSPAAAGVTVSHAHTLTDRLPKVAAVFAAGET
                     DWRTVQLAITRTEFVSDSVIERLDWRLATRVAKWHCWSRKRIINAIDAIVRVMDPDAI
                     RERVRREGKRHVKVIAQADGTAKLDGVVAAEAGIAFDKRLTELASAVCRDDPRTVDQR
                     RADALKPLSERRALACQCDDPNCPNRADDTAPSSRIVVNIIAGSETVLGGGTEPGYLE
                     GYGVIDADLVRRMAEDATLRLVEEPTVSTAAALRYQPSAALERAVRLRDLTCRFPGCD
                     RPAVICDLDHTIPFDHADPSKGGLTVAHNLKCLCRQHHRLKTFHDGWRDEQLPDGTVL
                     WTSPTGEVYRTVPGGVALFPDIGSPPACREPKAVRRNRSRERASRIARIRNRNRIHRP
                     INEAHRRLEYARRQEVGYRKHRNEMRKMLFILKGRPSTSPFCTWINDPLEPEELPPDW
                     RPPPEP"
     gene            1587..2324
                     /gene="dapB"
                     /locus_tag="VGP27_19875"
     CDS             1587..2324
                     /gene="dapB"
                     /locus_tag="VGP27_19875"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV7853592.1"
                     /translation="MRVGVLGSKGKVGTTMVRAVQDADDLTLSAEVDAGDSLDLLTRG
                     DTETVIDFTHPDVVMDNLKFCIENGIHAVVGTTGFDDKRLGQVRSWLTANPDVAILIA
                     PNFAIGAVLSMHFAQQAARFFDSVEVIELHHPHKADAPSGTAARTARLIAEARKDMPP
                     NPDATSTGLDGARGADVDGIPVHSVRLAGLVAHQEVLFGTEGETLTIRHDSLDRTSFV
                     PGVLLAVRKVKEHPGLTVGIEPLLDLT"
     gene            2321..2788
                     /locus_tag="VGP27_19880"
     CDS             2321..2788
                     /locus_tag="VGP27_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7853593.1"
                     /translation="MSTDGGRALRLQLVIGFMCVALVVYFLLLGRIAWAFMTSGSAAA
                     VGLGLAVLVMPLIGIWVMVSTLQAGLAHQRLARLAREKRMELDVSDLPTLPSGRIQRD
                     AADALFETVRDELEQDRDNWLRWYRLARAYDYAGDRSRAREAMKKAVELQEAE"
     gene            2785..3240
                     /locus_tag="VGP27_19885"
     CDS             2785..3240
                     /locus_tag="VGP27_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEV7853594.1"
                     /translation="MTKKLLIVHHTPSPHTHEMFEAVVAGATDPEIEGVEVLRRPALT
                     VSATDMLEADGYLLGTPANLGYMSGAMKHAFDVCYYPCLDATRGRPFGVYLHGNEGTE
                     GAERGIDSITTGLGWTKAADTVVVSGRPSKDDLEACWNLGATVAAQLME"
     gene            3297..3773
                     /locus_tag="VGP27_19890"
     CDS             3297..3773
                     /locus_tag="VGP27_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853595.1"
                     /translation="MPGIAELALGAAPIAGGALLGAAAGSFKGPDVRAMIAKDMDLLE
                     RIPDDQPELKARLKASIDERISDLVDATEKSRALREAAISYGGNWRDIVVFICAILFT
                     IVWWNVSHSRSNWLVMFIVMIILSVAAAIYAGRGIARAIANFLHRKDRDGEPPKTQ"
     gene            complement(3767..4693)
                     /locus_tag="VGP27_19895"
     CDS             complement(3767..4693)
                     /locus_tag="VGP27_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV7853596.1"
                     /translation="MRVDNEVSTTQDDADVGSRINHVLARSWLLVNGAQYDRFAPAAR
                     SRADIVVLDIEDAVAPKDKVSARGNVIKWLGDGNGDWVRVNGFGTQWWQDDLEMLSGT
                     SVGGVMLAMVESVDHVTETAKRLPNIPIVALVETARGLERITEIAAAKGTFRLAFGIG
                     DFRRDTGFGDNPTTLAYARSRFTIAAKAAHLPGAIDGPTVGSSALKLSEATAVSAEFG
                     MTGKICLTPDQCGTVNEGLSPSLDEITWAKEFFLEFERDGGEIRNGSDLPRIARANKI
                     LDLARAYGIEPSDFDDVDDPAHIPAPSDTYHY"
BASE COUNT          805 a         1520 c         1605 g          844 t
ORIGIN      
        1 tggttcgggg ggaggccgcc aatccggtgg caactcttcg ggttccagcg gatcgttgat
       61 ccaggtgcag aacgggctgg tgctgggtcg gcctttgaga atgaacaaca tcttgcgcat
      121 ctcgttgcgg tgcttgcggt agccgacctc ctgccggcga gcgtattcga gtcgccggtg
      181 tgcctcgttg attggccggt ggatccggtt gcggttgcga atgcgtgcga tgcggctcgc
      241 tctctcacga gaacggttgc ggcgaacggc tttcggctct cgacacgccg gcggcgaccc
      301 gatgtcgggg aacagggcga ccccgccggg cacggtccga tagacctctc cggtcggcga
      361 tgtccacagc acagtgccgt cgggcagttg ttcgtcgcgc cagccgtcgt ggaaagtttt
      421 gagtcgatgg tgttgccgac acaaacattt caggttgtga gccaccgtca agccgccctt
      481 gctcggatcg gcatggtcga acggaattgt gtggtccaaa tcgcagatca ccgcgggccg
      541 gtcgcatcca gggaatcgac atgtcagatc gcgtaagcgc accgcgcgtt ccagggccgc
      601 cgacggctga tagcgcagcg ctgcggcggt gctcaccgtc ggctcttcga cgagacgaag
      661 tgtcgcgtcc tcggccatcc ggcggaccag gtccgcgtcg attacgccgt acccttcgag
      721 gtatccaggc tcggtaccac cgccgagcac ggtttccgac cccgcaatga tattgaccac
      781 gatccggctc gacggagcgg tgtcatcagc acgattggga cagttggggt catcgcattg
      841 gcatgccagt gcacgacgct ccgataaagg cttcagtgcg tcggcgcggc gctgatcgac
      901 ggtgcgcgga tcgtctcgac acacggcgct ggcgagttcg gtgagccgct tgtcgaaggc
      961 tattccggct tcggccgcga cgacgccgtc gagtttggca gtgccgtcgg cctgcgcgat
     1021 gactttgaca tgacgcttgc cctcgcgccg cacccgctcc cggatcgcgt cgggatccat
     1081 cactcgcacg atggcgtcga tggcgttgat gattcgtttg cgtgaccagc agtgccactt
     1141 cgcgactcgg gtggcaagcc gccaatcgag gcgttcgatc accgaatcgc tgacgaattc
     1201 ggttcgggtg attgccaact gcaccgttcg ccagtccgtc tcacccgccg cgaacaccgc
     1261 cgccaccttg ggcagtcgat ccgtcagagt gtgggcatgg gagaccgtga cgcctgcagc
     1321 cgcgggggac aggttgcacg ccgcggcgac ttcggcggtg gtgcgttgaa atcccgtcac
     1381 gatcatgtag cccgggtcgg ggtcctctgt gtagacctcg gcggtccgca gcgcgagcag
     1441 ttcggccacc gccgacagac gccgcgccat cagtcgcgac tcctgacgct gggagtcggc
     1501 aattgccacc accaactcgg tcggcgccaa tccttcgaac atgtgttcga cagtacgccg
     1561 ggggtgcgac aaactagggt gggctcatgc gggtcggagt gctgggcagc aagggcaagg
     1621 tcggcacgac catggttcgg gcggttcagg acgccgacga cctcactctg tcggccgagg
     1681 tcgacgcagg cgattcgctc gatctgctga cccgaggcga caccgagacc gtcatcgact
     1741 tcacccatcc cgacgtggtg atggacaacc tgaagttctg catcgagaac ggcatccacg
     1801 ccgtcgtcgg caccaccggg ttcgacgaca agcggctggg acaggtccgc agctggctga
     1861 ccgcgaatcc cgatgtcgcc atcctgatcg cgccgaactt cgcgatcggc gccgtgctgt
     1921 cgatgcattt cgcgcagcag gcagcccgat tcttcgattc cgttgaggtc atcgagctcc
     1981 accatccaca caaggccgac gcaccgtcgg ggaccgccgc ccgcacggcg cggctcatcg
     2041 ccgaagcgcg aaaagacatg ccgcccaatc ccgatgccac cagcacaggt ctggacggcg
     2101 cccggggcgc cgacgtcgac ggcatacccg tgcattccgt gcggctggcc ggtctcgtcg
     2161 cgcaccagga ggtgttgttc ggcacggaag gcgaaacgct gacgatccgg cacgacagcc
     2221 tggaccggac gtcgttcgtg cccggtgtgc tgctcgcagt ccggaaagta aaggaacacc
     2281 ctgggctcac cgtcggcatt gagccgctgc tcgacctgac atgagcaccg acggcggccg
     2341 cgcactgcgc ctccagctgg tcatcggctt catgtgcgtc gcgctcgtcg tctatttcct
     2401 gttgctcggg cggatcgcgt gggcgttcat gacctccggc agcgccgcag cggtcggcct
     2461 gggcttggcg gtattggtca tgccgctgat cggcatctgg gtgatggtga gcacgctgca
     2521 ggccggcctc gcgcaccaac ggctggcgcg gctggcccgc gagaagcgca tggagctcga
     2581 cgtcagcgac ctgccgacgt tgccgtccgg ccggatccag cgcgacgcgg ccgacgcttt
     2641 gttcgagacc gtgcgtgacg agctggagca ggatcgagac aactggctcc ggtggtaccg
     2701 gctggcgcgg gcgtatgact atgcgggcga ccgcagccgt gcgagagagg cgatgaagaa
     2761 ggccgtcgaa ctgcaggagg cggaatgacc aagaagttgt tgatcgttca tcacacgccg
     2821 tcaccacaca cgcacgagat gttcgaggcg gtggtagcgg gcgcgaccga cccggagatc
     2881 gagggcgtcg aggtgctccg gcggccggcg ctgacggtgt cggcgaccga catgctcgag
     2941 gccgacggct acctgctggg cacccccgcc aacctcggct acatgagcgg ggcaatgaag
     3001 cacgcgttcg atgtctgcta ctacccgtgc ctggacgcca cgcgcgggcg accgttcggc
     3061 gtgtacctgc acggcaacga gggcacggag ggggccgagc ggggaatcga ctcgatcacg
     3121 accgggctcg gctggacgaa ggctgccgac accgtcgtcg tgtcaggaag gccgagcaag
     3181 gacgatctcg aggcgtgctg gaatctcggc gcaaccgtcg cggcccaact gatggaatga
     3241 ccccgtctgg gtgataacgg ttccgccggc gccgtcgaca cgatagaaag ctagttatgc
     3301 cgggtatcgc cgaactcgct ttgggcgcag cgccgatcgc gggtggtgcg ctgctgggcg
     3361 ccgctgcggg aagcttcaag ggaccggacg ttcgcgccat gatcgccaag gacatggact
     3421 tgctcgagcg gattccggat gatcagcctg agctgaaagc ccgactgaag gccagcatcg
     3481 acgaacgcat cagcgacctg gtcgatgcaa cggagaagag ccgcgccctg cgcgaggccg
     3541 caatctcata cggcggtaac tggcgcgaca tcgtcgtgtt catctgcgcg atcctcttca
     3601 cgatcgtctg gtggaacgta agccacagtc ggagcaactg gctggtgatg ttcattgtga
     3661 tgatcattct gtcggtagcg gcggccatct atgcgggccg cggtattgcg cgcgcaatag
     3721 ccaatttcct gcacaggaaa gatcgcgacg gggaaccgcc gaagactcag tagtggtagg
     3781 tgtccgacgg agccgggatg tgcgcggggt cgtcgacatc gtcgaaatcg gacggctcga
     3841 tgccgtaggc ccgggcgagg tcgaggatct tgttcgcacg cgcgatgcgc ggcaggtccg
     3901 agccgttgcg gatctcgccg ccgtcgcgct cgaactcgag gaaaaactct ttggcccagg
     3961 tgatttcgtc caacgacggc gaaagcccct cgttcaccgt tccgcactga tcgggcgtca
     4021 ggcagatctt gccggtcatg ccgaactcgg ccgacaccgc cgtcgcctca ctgagcttca
     4081 aggcgctcga cccgacggtc gggccgtcga ttgcgcccgg caggtgcgcg gccttggccg
     4141 cgatcgtgaa ccgcgagcgc gcgtaggcga gcgtggtcgg gttgtccccg aaaccggtgt
     4201 cgcggcggaa gtcgccgata ccgaaggcca gccggaatgt gccctttgcc gcggcgatct
     4261 cggtgattcg ctcgagaccg cgggccgtct ccacgagtgc gacgatcggg atgttcggta
     4321 gtcgtttcgc cgtctccgtg acgtggtcga ccgattccac catcgccagc atcaccccgc
     4381 cgaccgaagt gccagacaac atctcgaggt cgtcctgcca ccactgcgtt ccgaatccgt
     4441 tgacacggac ccagtccccg tttccgtcgc cgagccactt gatcacattg ccgcgtgccg
     4501 agaccttgtc cttgggtgcg accgcatcct ctatgtcgag gacgacgatg tcggcgcggg
     4561 accgcgcggc gggcgcgaac ctgtcgtact gcgcaccgtt gaccagcagc cagctcctgg
     4621 cgagcacgtg gttgattcgc gagccgacgt cggcgtcgtc ctgggtggtg ctgacctcgt
     4681 tgtccacgcg aatatcgtcg cttaccgcgg ccaccgggcc aagcgcgggg tctatccagg
     4741 tcagggcagc gcgccgaaca gcttctccag cgcg
//
LOCUS       DASYLX010000846         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1804556,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000846 DASYLX010000000
VERSION     DASYLX010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804556"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGP27_19900"
     CDS             <1..657
                     /locus_tag="VGP27_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853597.1"
                     /translation="GGVMLTATVIIGLYVQDVMGYSALRAGVAFLPFAGAFGLGTLLA
                     TRTAPVISPRWLIIGCGTLVLAAMLYGSTLDRSISYFPDFVVLIVVGGFGIGVISVIL
                     PLCAVADVGPREIGPVSAIQLMVYNLGGPLVLVVIQAVQTSRTLYLGGTTGPVHEMTS
                     SQLDALDHGYTYSLLWVAGIAVVVGAAALGIGFTARQIAAAQHTREAFEAGKLEEGKA
                     "
     gene            701..2032
                     /locus_tag="VGP27_19905"
     CDS             701..2032
                     /locus_tag="VGP27_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7853598.1"
                     /translation="MSVGRVQYARHGDVRLAYRVMGEGEIPIVLVPGWVSNVDLYDDP
                     TTVYAGIAERLSRHARLIVWDKRGTGLSDPVTHVPPIDERMDDLRAVLDAADVEFPAL
                     FGISEGGPMSIMFAATYPDRVRSLVLAGTTVRFAQELPDFPWGYTQEQIAAGLEDIDK
                     HWGEGALAELFFGPIADVPGVREMFGKEQRACASPMMARMMWQAVFEIDVRAVLGSVR
                     TPTLVMARRGDRIANFEGAKALAEGIPNAEFLEMPPGEHYASDLLDLFPQTVLKFVGQ
                     QADATPAERVLSTVLFTDIVGSTELLAAQGDAHWRRHLDDHDRLVDTVLARYGGRRAK
                     HTGDGIFALFDGPTKAARCGLDLVPALATHGIPIRAGVHIGECEKRGDEWSGMAVHVG
                     ARIGAMAGAGEVLTSRTVRDLSAGSGLVFEGLGAHRLKGLPEDTEVFRVKA"
     gene            2071..>3567
                     /locus_tag="VGP27_19910"
     CDS             2071..>3567
                     /locus_tag="VGP27_19910"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV7853599.1"
                     /translation="MITRMSELFLRTLRDDPADAEVPSHRLLIRAGYVRPVGPGLYSW
                     LPLGLRVLRKVEQVVRREMNAIGGQEILFPALLPRAPYETTNRWTEYGDGVFRLKDRR
                     DNDYLLGPTHEELFTLTVKGEYSSYKDFPVLLYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDVDDAGLDAVYHRHRDAYQRIFDELGVRYVIVSAVSGAMGGSASEEFLAESEI
                     GEDTFVRCLESGYAANVEAVVTAVPGAVPFDGLPEAKVHDTPDTPTIATLVDWANTVD
                     LGREITAADTLKNVLLKVRRPGGEWELLAIGVPGDREIDEKRLGAALDPAEYALLDDA
                     DFAKNPFLVKGYVGPKALRDNGVRYLVDPRIATGTSWITGADAPNKHVVGLVMGRDFT
                     ADGTIEAAEVRDGDPSPDGAGELVSARGIEIGHIFQLGRKYTDAFSVDVLGEDGKPVR
                     PTMGSYGIGVSRLVAVIAEQHHDDLGLRWPSSVSPFDAHVVIANKDEAA"
BASE COUNT          575 a         1137 c         1237 g          618 t
ORIGIN      
        1 ggcggcgtga tgctgaccgc gaccgtgatc atcgggctct acgtccagga cgtgatgggg
       61 tactccgcgc tgcgggccgg cgtcgcgttc ctgccgttcg cgggcgcctt cgggttgggc
      121 accctgctgg cgacgcgtac ggcgccggtc atctcgccgc gatggctgat catcggctgc
      181 ggcacgctcg tgctcgccgc gatgctgtac ggctcgacgc tggaccggtc gatctcgtac
      241 ttccccgact tcgtggtgct gatcgtggtg ggcggcttcg gaatcggcgt gatctcggtg
      301 atcctgccgc tctgcgcggt ggccgacgtc ggcccgcgcg agatagggcc ggtctcggcc
      361 attcagctga tggtctacaa cctcggcgga ccgctggtgc tcgtggtgat ccaggcggtg
      421 cagacgtcgc gcaccctcta tctgggaggc accacgggac cggtccacga gatgacgtcg
      481 agccaactcg acgcgttgga ccacgggtac acctactcgc tgctgtgggt cgcgggcatc
      541 gcggtggtgg tgggcgccgc cgcgctcggg atcgggttca ccgcacggca gatcgccgca
      601 gcccagcaca cccgcgaggc cttcgaggcc ggcaagctgg aagaaggcaa ggcgtagaaa
      661 tcttcgtcgc cgcgcctcgg tcggtgtcac acttggcgac gtgagtgtcg gacgtgtgca
      721 atatgcgcga cacggagacg tccggctcgc ttaccgggtg atgggcgagg gcgagatccc
      781 gatcgtgctg gtccctggct gggtcagcaa tgtcgatttg tacgacgacc ccaccaccgt
      841 ctacgccggt atcgccgagc ggctgagtcg gcatgcgcgg ctgatcgtat gggacaaacg
      901 cggaaccggg ctgtcggatc cggtgaccca cgtgccgccg atcgacgagc gcatggacga
      961 tttgcgggcc gtactcgacg cggccgacgt cgaattccct gcactgttcg gtatctccga
     1021 gggcggtccg atgagcatta tgttcgccgc gacatatccg gatcgcgtcc gttcgctggt
     1081 gctggcgggc acgacggtcc ggttcgccca ggaattgccc gattttccat ggggatacac
     1141 gcaggaacag atcgccgcgg gcctcgagga catcgacaaa cattggggcg agggcgcttt
     1201 ggcggagttg ttcttcggcc cgatcgccga cgtacccggt gtgcgcgaga tgttcggcaa
     1261 ggagcagcgc gcgtgcgcga gcccgatgat ggcacgcatg atgtggcagg ccgtctttga
     1321 gatcgacgtc cgtgctgtgc tcggcagcgt ccgcacgccg accctggtga tggctcgcag
     1381 aggggatcgc atcgcgaact tcgaaggcgc caaggcgctc gccgaaggta taccgaacgc
     1441 cgaattcctg gagatgccgc cgggcgaaca ctacgccagt gatctgcttg acctgttccc
     1501 acagacggtg ctcaaattcg ttggccagca agccgatgcg acgcccgccg agcgggtgct
     1561 gagcaccgtt ctgttcaccg atatcgtcgg ttcgacggaa ctgctcgccg cgcagggcga
     1621 cgcgcactgg cgccgacatc tcgacgacca cgaccggctg gtcgacacag tgttggcgag
     1681 atacggcggc aggcgggcga aacacaccgg cgacggaatc ttcgcgctct tcgacggccc
     1741 gacgaaggcg gctcgctgcg ggctggacct cgtgcccgca ctggccaccc acggcatccc
     1801 gatccgagcg ggcgtgcaca tcggcgagtg cgagaaacgc ggcgacgagt ggagcggcat
     1861 ggcggtgcac gtcggcgcac ggatcggtgc gatggccgga gcgggtgagg tgttgaccag
     1921 ccggacggtg cgagacctat ccgcaggctc gggcctggtc ttcgaaggct tgggcgccca
     1981 ccggctcaag ggattgcccg aggacaccga ggttttccgc gtcaaggcct gactcggcgc
     2041 cgaatcgagg cgccgataag cttgccgact gtgatcaccc gcatgtccga gctgttcctg
     2101 cgcacgctgc gcgacgaccc ggccgacgcc gaagtaccca gccacaggtt gttgatccgc
     2161 gccggctacg tccgcccggt cggacccggc ctgtacagct ggctgccgct gggcctgcgg
     2221 gtgctgcgca aggtcgagca ggttgtgcgc cgcgagatga acgccattgg cgggcaagag
     2281 attctgtttc ccgcgctgct gccacgggct ccgtacgaga cgacgaaccg gtggacggag
     2341 tacggtgacg gcgttttccg gctgaaggac cgccgcgaca acgactatct ccttgggccg
     2401 acccacgaag agctcttcac gctgaccgtt aagggggagt acagcagcta caaggacttc
     2461 ccggtgctgc tgtatcagat ccagacgaaa taccgcgacg aggctcggcc gcgcgcgggc
     2521 atcctgcgcg gacgcgagtt cgtcatgaag gactcctact ccttcgacgt cgacgacgcc
     2581 gggcttgatg cggtgtatca ccgccatcgc gacgcctacc agcgcatctt cgacgagttg
     2641 ggcgtccgtt acgtcatcgt gtcggcggtg tccggggcga tgggcggcag cgcgtcggag
     2701 gagttcctcg ccgaaagcga gatcggtgag gacaccttcg tgcgctgcct ggagtctggg
     2761 tacgccgcca atgtcgaggc cgtcgtgacc gccgtgccgg gcgctgtgcc gttcgacggt
     2821 ctgcccgaag ccaaagtgca tgacacgccc gacaccccta ccatcgccac cctggtcgat
     2881 tgggccaaca ccgtcgatct ggggcgcgag atcaccgccg cggacaccct gaagaacgtt
     2941 ctgttgaagg tgcgccggcc cggcggcgaa tgggaactgc tggcgatcgg tgtgcccggt
     3001 gatcgtgaga tcgacgagaa gcggctcggc gccgcgctcg atcccgccga gtacgccctg
     3061 ctcgatgacg cggactttgc caagaacccg tttctggtga agggatatgt cggcccgaag
     3121 gcattgcggg acaatggtgt tcgttatctc gtcgatcctc ggatcgcgac tggcacctcg
     3181 tggatcaccg gtgcggacgc acccaacaag cacgtcgtgg gtctggtcat gggtcgtgac
     3241 ttcaccgccg atggcacgat cgaggccgcc gaggtacgtg atggcgaccc gtcgccggac
     3301 ggggcgggcg agctggtgtc cgcccgcggc atcgagatcg gccacatctt ccagctcggc
     3361 cgcaagtaca ccgacgcatt ctccgtcgac gtgctcggcg aagacggcaa gccggtgcgg
     3421 cccaccatgg ggtcctacgg catcggggtg tcgcggctgg tggcggtgat cgccgaacag
     3481 catcacgacg acctcgggtt gcgctggccg tcgtccgtct cgccgttcga cgcccatgtc
     3541 gtcatcgcca acaaggacga ggccgcc
//
LOCUS       DASYLX010000847         5181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_269566,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000847 DASYLX010000000
VERSION     DASYLX010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269566"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VGP27_19915"
     CDS             <1..954
                     /locus_tag="VGP27_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEV7853600.1"
                     /translation="VEFAIEMGRLGGLGVLNGEGLIGRHADVAAKIAQVVEAAEKEPE
                     PAAAIRLLQQLHSAPLDPELLGAAVTRIREAGVTTAVRVSPQNAQALTPSLVAAGIDL
                     LVIQGTIVSAERVAQDGEPLNLKTFISELDVPVVAGGVLDHRTALHLMRTGAAGVIVG
                     YGSTSGVTTSDEVLGISVPMATAIADAAAARREYLDETGGRYVHVLADGDIHSSGDLA
                     KAIACGADAVVLGTPLSIAAESLGDGWFWPAAAAHPSLPRGALLQVAIGERPSLKQVL
                     TGPSDDPFGSLNLVGGLRRSMAKAGYCDLKEFQKVGLTVDV"
     gene            1195..2940
                     /locus_tag="VGP27_19920"
     CDS             1195..2940
                     /locus_tag="VGP27_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV7853601.1"
                     /translation="MQPDYDVLIVGSGFGGSVSALRLTEKGYRVGVLEAGRRYADEEF
                     AKTSWNLRKFLWMPKLGMYGIQRIHLLRNVMILAGAGVGGGSLNYANTLYVPPDPFFN
                     DPQWKHITDWRAELLPHYEQAQRMLGVVQNPTFTDADKVIKEVADDMGVGDTFVATPV
                     GVFFGPDGTKTPGKTVPDPYFGGAGPERTGCIECGSCMTGCRYGAKNTLVKNYLGLAE
                     SAGAQVHPMTTVTGFKQRSDGLWEVKTAHTGRLLRRKRRTFTATHLILAAGTFGTQKL
                     LFKMRDRGNLPKLSDKLGVLTRTNSESIVGAGRLSVRDDLDLTHGVAITSSIHPTPDT
                     HVEPVRYGKGSNAMGLLQTLMTDGAGEGGTDTPRWRQLLDQAREDPKNMLRLLNPARW
                     SERTMIALVMQHLDNSITTYTKRTKLGFRRMVSKQGHGEPNPTWIPVGNEVTRRLAQK
                     IDGVAGGTWGELFNIPLTAHFLGGAAIGDSQEHGVIDPYQRVYNYPTMYVVDGASVSA
                     NLGVNPSLSITAQAERAASLWPNKGEDDLRPKQGEPYKRLAPIEPEHPVVPADAFGAL
                     RRISFEPGQPVSSAG"
     gene            3013..3672
                     /gene="phoU"
                     /locus_tag="VGP27_19925"
     CDS             3013..3672
                     /gene="phoU"
                     /locus_tag="VGP27_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV7853602.1"
                     /translation="MRTAYYKEISALCEQLSESCGLAADAMEHATSALLDADLSLAEQ
                     VIADHQHIVALSQRAEGTALKLLALQAPVAGDLRKIVGSIHISADVERMGALAVHVAS
                     ISRLRHPVCALPADVRSSFAEMGARSVELARTAREVLLSSDHGKAGRLQDEDDAVDAE
                     HRHLFTLLIDHKWQGGVCSAVDVALLGRYYERFADHAVEIGRRVVFEETGGLPVHKEL
                     A"
     gene            complement(3690..4601)
                     /locus_tag="VGP27_19930"
     CDS             complement(3690..4601)
                     /locus_tag="VGP27_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7853603.1"
                     /translation="MPEWVVVVLALAAAVASALGIVIRQRATLEIPQDEGVSTTMFKK
                     LLRNRLWWAGTAVAALGYGFQALALTWGSLILVQPLLVSALLFALPMSARMAHRRVTT
                     HDWVWALVLTFGLATFVLTAKVQAGNDRPLPAVWILATVLCVAVVLGCVVAGARTAGR
                     SRALLIATAVGVLFGVVSVLTKVTVQRLNEEGLWGTLVVPAPYLVVTLGVAATLLQQS
                     AFHAGALQASVPTMLVLEPLVAVSLGVIVLGEALAVTDPRTIATLALAVAAMATATIA
                     LGRDEGAFEEELEAQMARRSEHLPPEK"
     gene            4667..>5181
                     /locus_tag="VGP27_19935"
     CDS             4667..>5181
                     /locus_tag="VGP27_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853604.1"
                     /translation="MVWRVNTEGVLDGKVAIVTGTSRGVGVGIAHELLRAGATVVGCS
                     RSKLGAIPGADQYQGWLQRSAQLVCDQGDFHAIDDLVAEVADTYGRIDILVNNAGGTV
                     PAPNVEDVPELVQRIQGAPRADDDFERTALFHSFAIQMNLISPLWFALRVYRQMRGQD
                     GVGSIINISSGA"
BASE COUNT          911 a         1752 c         1694 g          824 t
ORIGIN      
        1 gtggagttcg ccatcgagat gggccgcctc ggtggtctcg gcgtgctcaa cggcgagggg
       61 ctgatcggaa ggcacgccga cgtcgcggcc aagatcgccc aggttgtcga ggccgcggag
      121 aaggagccag aacccgctgc ggcgattcgc cttctgcaac aactacattc ggcgccgctg
      181 gaccccgagc tgctcggtgc ggccgtcacg cgaatccgcg aagccggggt gacgaccgcg
      241 gtccgggtga gcccgcagaa cgcccaggcg ttgacgccgt cgctggtcgc cgcgggcatc
      301 gaccttctgg tgatccaggg caccatcgtg tcggccgaac gcgtcgcgca ggacggtgag
      361 ccgctgaacc tgaagacgtt catctccgag ctggacgtgc cggtcgtggc gggcggcgtg
      421 ctggaccacc gcaccgccct tcacctgatg cgcacgggcg cggcgggtgt gatcgtcggc
      481 tacggctcca ccagcggcgt caccaccagc gacgaagtgc ttggcatcag cgtgccaatg
      541 gcgaccgcga tcgcggacgc ggccgctgcc cgtcgggagt atcttgacga gaccggcggc
      601 cggtacgtgc atgtgctggc cgacggcgac atccactcgt ccggagacct ggccaaggcc
      661 attgcgtgcg gcgccgacgc cgtcgtgctc ggcaccccgt tgtcgatagc cgccgagtcg
      721 cttggcgacg gctggttctg gcctgccgcc gcggctcacc cgtcgctgcc ccgaggcgcg
      781 ctactccagg tcgcgatcgg cgagcggccg tcgctgaagc aggtactcac cgggccgtcg
      841 gacgacccgt tcggctcgct gaacctcgtc ggcggcctgc gccggtcgat ggccaaggcc
      901 ggctactgcg acctcaagga gttccagaag gtcggcctga ccgtcgatgt ctagcccggc
      961 agctcgtcgc cgcccggagc cgtcgccgcc cggcgccaac cgccccgccg atgacgagtg
     1021 ccgccattag cggcgcgagg tgacggatgt ccggtgtcat gggtgtgcct cttttattct
     1081 cgctggtgag ccacggatat gcccggtcgc cgggaacggc tgccgacgcg cttcgaggtg
     1141 gctgaggcgg acccgggcta gtggttactg gccggtaagt tcatactggg ttttatgcag
     1201 cctgactatg acgttctgat cgttggttcg gggttcgggg gcagtgtttc cgcgttgcgg
     1261 ctcaccgaga agggctaccg cgtcggtgtc cttgaagccg gtcgccgcta cgccgacgag
     1321 gagttcgcca agacctcgtg gaacctcagg aagttcctct ggatgccgaa gctcggtatg
     1381 tacgggatcc agcgaatcca cctgctccgc aacgtgatga tcctggccgg cgcaggcgtc
     1441 ggcggcggat cgctgaacta cgccaacacg ctctatgtgc cgccggaccc gttcttcaac
     1501 gatccgcagt ggaagcacat caccgattgg cgcgccgagt tgctgccgca ctacgagcag
     1561 gcgcagcgca tgctcggcgt ggtccagaac ccgacgttca ccgacgccga caaggtgatc
     1621 aaagaggtcg ccgacgacat gggcgtcggc gacacgttcg tggcgacgcc cgtcggggtg
     1681 ttcttcgggc ccgacggcac caagacgccg ggcaagaccg tgcccgaccc gtacttcggt
     1741 ggggcgggcc ccgagcgcac cggctgcatc gagtgcggtt cgtgcatgac gggatgccgc
     1801 tacggcgcca agaacaccct cgtcaagaac tacctgggcc tcgcggaatc cgctggtgca
     1861 caggtgcacc cgatgacgac ggtgacgggg ttcaagcaac ggtccgacgg gctgtgggag
     1921 gtcaagaccg cacacaccgg caggctgctg cgccgcaagc ggcgtacctt caccgccacc
     1981 catctcatcc tcgccgcggg cacattcggc acccagaagc tgctgttcaa gatgcgcgac
     2041 cgcggcaacc tgccgaagct gtcggacaag ctgggcgtgt tgacccgtac caactccgag
     2101 tcgatcgtcg gtgcggggcg cctctcggtg cgcgacgacc tcgacctgac ccatggtgtg
     2161 gcgatcacgt cgtcgattca cccgaccccc gacactcacg tcgagccggt tcggtacggc
     2221 aagggctcca acgccatggg gcttctgcag acactgatga ccgacggtgc gggcgaaggc
     2281 ggtaccgaca ccccgcgctg gcggcagctg ttggaccagg cccgtgagga tccgaagaac
     2341 atgctgcggc tgctgaaccc ggcccgctgg agcgagcgga ccatgatcgc gctggtgatg
     2401 cagcacctgg acaattcgat caccacgtac accaagcgca ccaagctcgg cttccgccgg
     2461 atggtcagca aacagggcca cggcgaaccc aacccgacct ggatcccggt gggcaacgag
     2521 gtcacccgac gcctcgcgca gaagatcgac ggagtggcgg gcggcacctg gggagagctg
     2581 ttcaatatcc cgctgaccgc gcatttcctc ggtggcgcgg cgataggcga tagccaggag
     2641 cacggcgtga tcgacccata ccagcgcgtg tacaactacc cgacgatgta cgtggtcgac
     2701 ggcgcttcgg tctcggccaa cctcggtgtc aacccgtcgc tgagcatcac tgcgcaggcc
     2761 gaacgcgcgg cgtcgctgtg gccgaacaag ggcgaagacg atctgcggcc caagcagggt
     2821 gagccctaca agcggctggc ccccatcgag ccggagcatc ccgtcgtacc ggccgacgcc
     2881 ttcggcgcac tgcgccggat ttcgttcgaa ccgggccaac ccgtcagctc cgccggttaa
     2941 gcaccccatt tccgggtatt tactctgtac gccgggctgc gatggtctcg gcgcacggaa
     3001 ggttttgggg ccatgcggac cgcgtactac aaggaaatat ccgcgctctg cgaacagctg
     3061 agtgaaagtt gcggcctggc cgcagacgcg atggagcatg ccacgtccgc tttgttggac
     3121 gccgatctgt cgctggccga acaggtgatc gccgaccacc agcacatcgt ggcgttgagc
     3181 cagcgcgccg agggaactgc gctcaaactg ctcgcattgc aggcgccggt agccggtgat
     3241 ctgcgcaaga tcgtgggttc gatccacatc agcgccgacg tggaacggat gggcgcactc
     3301 gccgttcacg tcgcgagtat ctcccggctg cgtcatcccg tctgtgcgct gcccgccgac
     3361 gtccgatcga gcttcgccga aatgggagcc agatcagtcg aattggcgag aacagcgcgg
     3421 gaagtgttgc tctcgagcga ccacggcaag gccggtcggc tgcaggacga ggacgacgcc
     3481 gtcgacgccg agcaccgaca cctgttcact ctgctgatcg accacaaatg gcagggtggg
     3541 gtgtgttccg cggttgatgt cgcgttgctt ggccgctatt acgaacgatt cgccgaccac
     3601 gccgtcgaga tcggcaggcg ggtggtcttc gaggagaccg gtggcctgcc cgtgcacaag
     3661 gaactggcct agaacgactg cccgcctccc tacttttcgg gcggcaggtg ctcagaccgc
     3721 cgcgccatct gcgcttcgag ctcctcttcg aacgcgccct cgtcacgacc cagggcgatg
     3781 gtcgccgtgg ccatcgccgc aacagcgagg gcgagcgttg cgatcgtccg cgggtcggtg
     3841 accgcgagcg cctcaccgag gacgatgacg cccaacgaca ccgcgaccaa gggttcgagc
     3901 accagcatgg tgggtaccga cgcctgcagc gcacctgcgt gaaaagccga ctgctgcaac
     3961 aacgttgccg cgacgccgag ggtcaccacg agatacggcg cgggcacgac cagcgtgccc
     4021 cagaggcctt cctcgttcag ccgctgcacg gtgactttgg tcagcaccga gaccacgccg
     4081 aacagcaccc cgacggcagt tgcgatcagc agagcacgac tgcgacccgc ggtgcgggct
     4141 ccggcgacca cgcatcccag caccacggcc acgcacagca ccgtcgccaa tatccagaca
     4201 gcgggtagcg gacgatcgtt tccggcctgc accttcgccg tcaagacgaa tgtcgccaag
     4261 ccgaacgtca gcaccaacgc ccacacccag tcatgggtgg tgacgcggcg atgggccatc
     4321 cgggcactca tcggcagcgc gaacagcagc gccgacacca acagcggttg cacgagtatc
     4381 agcgagcccc aggtcaatgc gagtgcctgg aagccgtagc ccaatgccgc caccgcggtg
     4441 cccgcccacc acagacggtt ccgcagcagc ttcttgaaca tcgttgtgct gacaccctcg
     4501 tcctgaggga tttccagcgt ggcgcgctga cggatcacga tcccgagtgc cgaggccact
     4561 gcggcggcca acgccagcac aacgaccacc cactcgggca tgaatctcct catctgctgt
     4621 cgacaagctg cttacgggta cccaatcaca cgtcgcacgc tcgtctatgg tttggcgggt
     4681 gaacaccgaa ggtgtcctcg acgggaaagt ggccatcgta acgggcacca gccgcggagt
     4741 cggggtgggc atcgcacacg aacttctccg cgccggcgcc accgtcgtcg gctgctcccg
     4801 gtcgaaactc ggcgccatcc ccggtgccga tcaataccag gggtggctgc agcgcagcgc
     4861 ccagttagtc tgcgaccaag gcgatttcca cgccatcgac gacttggtcg ccgaagtggc
     4921 cgacacctac ggtcgcatcg acatcctggt caacaatgcg ggcggcacgg tgcctgcgcc
     4981 caacgtcgaa gacgttcccg agttggttca gcggatccag ggcgcccctc gggccgacga
     5041 cgacttcgag cggacagcgc tgtttcactc cttcgccatc cagatgaacc tgatcagccc
     5101 gctgtggttc gccctgcgcg tctaccggca gatgcgcggc caggacggcg tcgggtcgat
     5161 catcaacatc tccagcggcg c
//
LOCUS       DASYLX010000848         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3887876,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000848 DASYLX010000000
VERSION     DASYLX010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887876"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGP27_19940"
     CDS             complement(<1..849)
                     /locus_tag="VGP27_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7853605.1"
                     /translation="MELGVYTAVLHDQPLASALRTIRSLGLRGAEINAGGFLPTPHLP
                     VDALLENRMEPAEYLADFESTDVKLTGLNVNGNPLHADPEVGPEDADDLRRAIRVAAK
                     LGVHRVVTMSGLPAPHAGGQWPAWHVNPWDSGYLDSLDYQWEDVAVPFWREIDALARD
                     HDVKVCIEMHPQNLVFNPPTLQRLVEKIGATHVGAEMDPSHLFWQGIDPVAAIAYLGD
                     LVFHAAAKDTRINPACAVYGVLDDRFTRTAAKDNPTSLGGRHTVNRWPKESSWDFVAV
                     GRGHDVQ"
     gene            949..1977
                     /locus_tag="VGP27_19945"
     CDS             949..1977
                     /locus_tag="VGP27_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7853606.1"
                     /translation="MTVTPAEHDRSRRRPTMADVAARAGVSRTLVSFILDGKPGASAK
                     TKQRVLAVAEEIGYQPDSAARLLARGRSRTLGVLMDVTQLFEAELVTGIYPAAEELGY
                     EVLLSANLPDRGESVPIDALLSHRCGGLILLGPSSQLGYLSSLAEKVPVVVVGRRLTA
                     QPGLATVRTDDAKGIRQAVDYLVGLGHRDIHHVDGGTDPGSSERRQAYEAAMRLHGLS
                     DRARVIAGAHNESAGADAARSMLAEPPLPTAVLAGNDRCALGLLDVFTRAGLDVPGDM
                     SLIGFDDSHLSDNPRIDLTTVHQDAAAMARHAVLLAVAMLEGRPADDHDVVLEPTLVI
                     RGTTAPPR"
     gene            complement(2053..>2439)
                     /locus_tag="VGP27_19950"
     CDS             complement(2053..>2439)
                     /locus_tag="VGP27_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEV7853607.1"
                     /translation="DEEVARELSLKDDREVVAIHRLRSANGEPLAVMTNHLPVGIAPD
                     AEELESNGLYQSLRARGVHIRLARQRIGARGATRAEAKLLDEKPGAPLLTMARTAFDD
                     SGAAVEFGTHVYRASRYYFDTTLVDR"
BASE COUNT          406 a          786 c          826 g          421 t
ORIGIN      
        1 ctggacgtcg tggccgcggc cgaccgcgac gaagtcccag ctcgattcct tcggccaccg
       61 gttgacggtg tgcctgccgc ccaaggacgt cgggttgtcc ttcgcggccg tcctggtgaa
      121 ccggtcatcg agcacgccgt atacggcgca tgccgggttg atgcgggtgt ccttggccgc
      181 ggcatggaag acgaggtcgc caaggtaggc gatcgccgcg acagggtcga tgccctgcca
      241 gaacaggtgg ctggggtcca tctcggcgcc gacgtgcgtg gcaccgatct tctcgacgag
      301 tcgctgcagt gtcggtggat tgaagaccag gttctgcggg tgcatctcga tgcagacctt
      361 gacgtcgtgg tcgcgggcca acgcgtcgat ctcccgccag aacgggacgg cgacgtcttc
      421 ccactgataa tccagcgagt cgagatagcc gctgtcccag gggttcacgt gccatgccgg
      481 ccattggccg cctgcatgcg gggcgggcag cccggacatg gtgactaccc ggtgcacacc
      541 gagcttggcg gcgacacgga tggctcgacg taaatcgtcg gcatcttcgg gacccacttc
      601 tgggtcggcg tgtagtgggt tcccgttgac gttgagaccg gtcaacttga cgtcggtgga
      661 ttcgaagtcc gcgaggtact ccgcgggctc catccggttc tcgagcagag cgtcgacggg
      721 cagatgcggt gtcggcaaga aaccgccggc gttgatctcg gccccgcgaa ggccgagaga
      781 gcggatcgtg cgcagggcgg acgccagtgg ttgatcgtgc agcacggcgg tgtagacgcc
      841 cagttccatc gaaatcgact ccctctaaag cgcgttagta acgcgcgtta gtgatactat
      901 gaaccggtcc tgcgcttgtg tcaaccgtca cagccgagga gaacgaggat gaccgtcaca
      961 ccggccgagc acgaccgctc gcggcgcagg cccacgatgg cggacgtcgc agcaagggca
     1021 ggcgtgtcgc gcacgctggt ctcgttcatc ctcgacggca agccgggagc gagtgcgaag
     1081 accaagcagc gggtgcttgc ggtcgccgag gagatcggct atcaacccga ttcagcggcc
     1141 cggctgctgg cccgtggtcg cagccgcacc cttggcgtgc tgatggacgt gacccagctg
     1201 ttcgaggccg agttggtcac cggcatctac cccgccgccg aagaactcgg ctatgaggtt
     1261 ctgttgtcgg ccaacctgcc cgatcgtggc gagtcggttc cgatcgacgc cctgctcagc
     1321 caccgctgcg gcggcctgat cctgctcggc cccagctcgc aactcggcta tttgtcgtcg
     1381 ctcgccgaaa aagtccctgt ggtggtggtc ggacgtcgac tcaccgccca acccgggctg
     1441 gccaccgtac gcaccgatga cgcgaaaggc atccggcagg cagtggacta cctggtcggt
     1501 ctaggacacc gggacatcca ccacgtggac ggtggcaccg atcccggttc gtcggagcgg
     1561 cgtcaggcct acgaggcggc gatgcgcctc cacgggctgt ccgatcgcgc gagagtgatc
     1621 gcgggcgcgc acaacgagtc ggcaggtgca gacgctgcgc ggtcgatgct ggccgaaccg
     1681 ccgttaccga cggcggtact ggccggcaat gaccgatgtg ctcttggcct gctcgatgta
     1741 ttcaccaggg ccggcctcga cgtgccgggc gacatgtcgc tgatcggttt cgacgacagt
     1801 catctgtccg acaatccgcg aatcgacttg acgaccgtgc accaggacgc cgccgcgatg
     1861 gcgcgccatg cagttctgct ggcggttgcc atgctcgagg gtaggcccgc cgacgatcac
     1921 gacgtagtgc tggaaccgac gctggtgatt cgcggcacga ccgcgccacc gcgatgaccg
     1981 tgaaacggaa ttccagcagc ggaagttgcc gagagtaaac ccctgctgga attccgtttc
     2041 gcgtagagaa agtcagcggt cgacgagtgt ggtgtcgaag tagtagcgcg atgcgcgata
     2101 cacgtgcgtg ccgaactcga cggcagcgcc ggagtcgtcg aacgccgtcc gcgccatcgt
     2161 cagcagcggc gcgccgggct tctcgtcgag cagcttggcc tccgcccggg ttgctccgcg
     2221 agcgccgatg cgttgccggg ccaggcggat gtgcaccccg cgggcgcgca gcgactgata
     2281 caggccgttg ctctccagct cctcggcatc cggggcgatg ccgaccggca aatggttcgt
     2341 catcaccgcc aacggctcgc cgttggccga gcgcaagcgg tgaatcgcca cgacttcgcg
     2401 gtcgtccttc aagctcaact cgcgcgcgac ctcctcgtc
//
LOCUS       DASYLX010000849         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_487230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000849 DASYLX010000000
VERSION     DASYLX010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VGP27_19955"
     CDS             <1..1705
                     /locus_tag="VGP27_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEV7853608.1"
                     /translation="ANVGDTVAYRYLDYTRSERGFAHELTWNQLGIRIRAIGAQVQRV
                     ASRGDRVAVLAPQGLDYVAGFFAAIKAGTVAVPLFAPELQGHAERLAIALHDSRPTVV
                     LTTSPASAAVEAALDEIGLTPRPHVITIDDVPDSLGDGLVPVHLDAGDISHLQYTSGS
                     TRDPVGVEITHRAVGTNLTQMILSIDLLDRNTHGCSWLPLYHDMGLSMIGFPTVYGGH
                     STLMSPAAFVRRPERWIRALAAGSRQGRVVTAAPNFACEYAAQRGLPAAGDDIDLSNV
                     VLILGSEPVSRRAIDVFNAAFAPYGLPTTAIKPSYGIAEATLFVSTIAPDAAPTVAHL
                     DRGMLEKGDAVRVAADSPDAAEHVSCGQIARGQWSVIVDPEDRTELSDGRVGEIWLHG
                     DNVGRGYWERPEETHQTFGITLLSDISEGSHACGAPADATWLRTGDLGFYLDGELYVT
                     GRIADVITVGDRLLYPEDVEATVADASPIVRRGYAAAFTAPSGAGEQLVIVAERASGT
                     RRADPAPAVDAIRAQVSRHHGLSVGDVRFVPAGAIPRTTSGKLARRACRTAYLGGEFG
                     N"
     gene            1789..2973
                     /locus_tag="VGP27_19960"
     CDS             1789..2973
                     /locus_tag="VGP27_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853609.1"
                     /translation="MTSMTEAADRVVGLARGMRDLVRAEAAESERNRTLTPAVVKEMW
                     TSGLMSAFNPRAAGGVEPAFREMIETWIEMAWQDGSFGWIGIANMPSSFAAAAYLPDD
                     GFAEVFTAHANRVTMGGQFFPNGQGRVIDGGYRLTGSWSFGSGTGHSQYVAAGFFPMD
                     DSEMRWISEGVPDMQVAVVPREEISFNDGWFVQGLKGTGSYDYSADDVFIPAHRTFAL
                     FSREPLRGSSPATRMGLMPATAAGHASWALGVAKSMLDDVQELAATKYRMSDMASLAS
                     RPTFQKGLAQHVSAWRAARLLVLDAFTTAESAVATGQDLGPMLRADMRAAAVYATDVA
                     RQCAEWAHLVAGTSSIREGSRLERAFRDIYTGTQHAFISEKVAIDAAQIWLGVIEDQF
                     GL"
     gene            3001..>3568
                     /locus_tag="VGP27_19965"
     CDS             3001..>3568
                     /locus_tag="VGP27_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7853610.1"
                     /translation="MRDRTAGLSVAARHAHEFLNGLDDRPVAARVDAAGVRDALGGPL
                     PEQGEDVDAVIDALAAGADPGLVGTAGPRHFGFVIGGALPAALAADWLVSAWDQNAAF
                     HALSPAAAAIEEVTSAWTLDLFGLPGDASVGFVTGGQGANTTCLAAARHAVLAAAGWN
                     VEDDGLIGAPPVRILCGEAAHATIYTALR"
BASE COUNT          554 a         1254 c         1193 g          567 t
ORIGIN      
        1 cgcgaacgtc ggcgacaccg tggcgtaccg ctacctggac tacacccgtt cagagcgtgg
       61 gttcgcccac gaactgacct ggaaccaact cggcatccgg attcgggcca tcggcgcgca
      121 ggtgcaacgg gtcgcgtccc gtggggaccg ggtcgcggtc ctggccccgc agggtctgga
      181 ttacgtcgcc gggttcttcg ccgccatcaa ggcgggcacc gtcgcggtac cgctgttcgc
      241 cccggagttg cagggtcatg ccgagcgcct cgcaatcgca cttcacgact cccgccccac
      301 cgtcgtcctg acgacatccc cggcatcggc cgccgtcgag gcggcgttgg atgagatcgg
      361 cctgacgccc agaccgcacg tcatcaccat cgacgatgtt ccggactcgc tcggcgacgg
      421 gttggttccc gttcacctcg acgccggcga catctcacat ctgcagtaca cgtcgggatc
      481 cacgcgggat cctgtcggcg tcgagatcac ccaccgggcg gtcggcacca acttgacgca
      541 gatgatcctg tccatcgatc tcctcgaccg aaatacccac ggctgcagtt ggttaccgct
      601 ctaccacgac atgggccttt cgatgatcgg ctttcccacc gtctacggcg gccactcgac
      661 gctgatgtca ccggccgcgt tcgttcgacg gcccgagcgt tggatccggg cgttagcggc
      721 cggctcccgg cagggccgcg tcgtcaccgc cgcgcccaac ttcgcttgcg agtacgccgc
      781 gcagcgcggc ctccccgcgg cgggcgacga catcgacctg agcaacgtcg tgctgatcct
      841 cggttcggaa ccggtgagca ggagggccat cgacgtcttc aacgcagcgt tcgcgccata
      901 cgggctgccg accaccgcga tcaagccgtc atacggcatc gccgaggcga ccctgttcgt
      961 ctctaccatc gcccccgacg ccgcgcctac cgtcgcccac ctggaccgcg ggatgctgga
     1021 gaagggtgac gcggtgcgtg tcgcggcgga cagtcccgac gccgcagaac atgtgtcctg
     1081 cggacagatc gcccgcggcc aatggtccgt catcgtggat cccgaagacc gcaccgagct
     1141 ttccgacggc cgcgtcggcg agatctggct gcatggcgac aatgtcggcc gtggctactg
     1201 ggagcggccc gaagaaacac accagacgtt cggtatcact ctgctgtcgg atatcagcga
     1261 gggcagccac gcgtgcggcg ccccggccga tgcgacctgg ctgcgaaccg gtgatctggg
     1321 gttctacctc gacggcgagc tctacgtcac cggccgcatc gcggatgtga tcaccgtcgg
     1381 cgaccgcctg ctctacccag aggacgtcga ggccaccgtc gcggacgcgt cgccgatcgt
     1441 gcgccgcggc tatgcggccg cgttcaccgc gccctccggc gcgggcgagc aactggtgat
     1501 cgtcgcggaa cgcgcgtcgg gcacgcggcg cgccgacccc gcaccggcgg tcgacgccat
     1561 ccgcgcgcag gtgtcacgac accacgggct gtccgtcggc gacgtcaggt tcgttcccgc
     1621 aggggcgatc ccgcgcacca cgagcggcaa actggcccga cgggcttgcc gtaccgcata
     1681 cctgggaggc gagttcggga actagctcga cggcgtcgcc gatcaggatc gcgcgaagtc
     1741 tgcggaagac agccgtccac cgaacggcgc gcggctaggg tcagccgaat gacgtcgatg
     1801 accgaagcgg ctgaccgggt tgtcgggttg gcgcgcggaa tgcgggacct cgtgcgggcg
     1861 gaagccgcgg aatccgaacg caaccgaacg ctgaccccgg cggtcgtgaa ggagatgtgg
     1921 acatccggtc tgatgtccgc gttcaaccca cgtgcggcgg gcggtgtcga accggcattc
     1981 cgggagatga tcgagacctg gatcgaaatg gcttggcagg acggctcgtt cggatggatc
     2041 ggtatcgcga acatgccgtc gtcgttcgcg gccgcggcgt atctccccga cgacggcttc
     2101 gccgaagtgt tcaccgctca cgccaaccgc gtcacgatgg gaggacagtt cttcccgaac
     2161 ggccagggca gggtcatcga tggtggctac cggctgaccg gatcgtggag cttcggttcg
     2221 ggcactggcc attcccagta cgtcgccgcc ggcttctttc cgatggacga ttcagagatg
     2281 cgctggatca gcgaaggtgt gcccgatatg caggtcgccg tggtgccgcg agaggagatc
     2341 tcgttcaacg acggctggtt cgtgcagggc ctcaagggaa ccggctccta cgactacagc
     2401 gccgacgatg tcttcattcc cgcgcaccgc accttcgccc tgttctctcg cgaaccgttg
     2461 cggggaagtt caccggccac ccgcatgggg ctgatgcccg ccaccgccgc cggtcacgca
     2521 tcgtgggcgc tcggcgttgc caagagcatg ctcgacgatg tccaggagct ggccgccacg
     2581 aaataccgga tgagcgatat ggcatcgctg gccagtcgcc ccaccttcca gaaaggcctt
     2641 gcgcagcacg tctcggcgtg gcgggcagcc cgcctcctgg tactcgacgc gttcaccacc
     2701 gctgaatccg ccgtcgcgac aggccaagac ctggggccga tgctgcgcgc cgacatgcgg
     2761 gccgccgccg tctatgccac agacgtggca cgccaatgcg ccgaatgggc gcacctcgtc
     2821 gcgggcacca gctcgatacg cgagggcagc cggctggagc gggcgttccg cgacatttac
     2881 accggcaccc agcacgcctt catcagcgag aaggtggcga tcgacgcggc gcagatctgg
     2941 ctcggcgtca tcgaggacca attcggcctg tgagcggcga gcgccgatag ggttttattc
     3001 atgcgcgatc gcaccgcggg cctgtccgtc gccgcgcggc atgctcacga attcctcaac
     3061 ggtctggacg accgcccggt ggccgcccgc gtcgacgccg cgggggtgcg cgacgcgctc
     3121 ggtggcccgc ttcctgagca gggggaggac gtcgacgccg tcatcgacgc gctcgccgcg
     3181 ggcgccgacc ccggtctggt gggcaccgca ggcccgcgcc acttcggctt cgtcatcggc
     3241 ggtgcgctgc ccgccgcact ggccgcggac tggctggtct cggcctggga ccagaacgcc
     3301 gccttccacg cgctgtcgcc cgcggctgcg gcgatcgaag aggtcacctc cgcatggaca
     3361 ctcgacctgt tcgggctgcc cggcgatgcc tctgtcggct tcgtcaccgg cgggcagggc
     3421 gcgaacacaa cctgtctggc cgccgcccgc cacgcggtgc tcgcggcggc cggctggaat
     3481 gtcgaagacg acgggctcat cggtgcgccg ccggtacgca tcctgtgcgg tgaggcggcc
     3541 cacgcgacga tctacaccgc gctgcgcc
//
LOCUS       DASYLX010000850         2481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1804897,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000850 DASYLX010000000
VERSION     DASYLX010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804897"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VGP27_19970"
     CDS             <1..870
                     /locus_tag="VGP27_19970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7853611.1"
                     /translation="TSTIELGTSIAVAFARNPMTVANIGWDLQAFSQGRFILGLGSQI
                     KPHIENRFSMPWGRPVERMREFVLALGEIWSCWQAGTKLAFEGDFYTHKLMTPMFTPE
                     PLAHALPKVFVAAVGEAMTEMCGEVADGLLAHAFTTKRYFDEVTTPALLRGMERSGRK
                     RSEFELSCPLFVVTGNDDAELTTNAVGTRKQIAFYASTPAYRRVLELHGWGDLQTELH
                     RMSKEGQWDAMGSLIDDDILNEFAVVAPLNDVAQVIRDRCDGVIDRVLVGFPASVDDA
                     AVATVLDEMRSRA"
     gene            882..2102
                     /locus_tag="VGP27_19975"
     CDS             882..2102
                     /locus_tag="VGP27_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853612.1"
                     /translation="MDEAAKVFATPKAYTDERKLHSALTHLRADAPVSWVDVPSYEPF
                     WAITKHADIMDIERANHVFTNYPRPVLVTAEDDERQAAVGVRTLIHMDDPQHRGVRAI
                     GADWFRPKAMRALKNRADELAKVYVDNMAAIGSECDFVQQVAVNYPLYMIMSLLGVPE
                     SDFPLMLKLTQELFGSDDDEFKRGTDGGDQMSALLEMFEYFTALTASRREDPTEDLAS
                     AIATATIDGEPLTDIDTISYYAIIAAAGHDTTSATISGGMLALIENPDQRRMLTADLD
                     LMPLATEEMIRWVTPVKAFMRTATEDATVRGVPIAAGQSVLLSYVSGNRDEDVFDDPF
                     RFDVTRDPNKHIAFGYGVHFCLGAALARMEVGSFFSELLPRLESVELAGDPQHVATTF
                     VGGIKHLPIRYSLR"
     gene            complement(2158..>2481)
                     /locus_tag="VGP27_19980"
     CDS             complement(2158..>2481)
                     /locus_tag="VGP27_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HEV7853613.1"
                     /translation="PDGPPPGDFNRGPGDYNRPGGHDWRPPWNPQDNDWRGRFRDAPW
                     GNDLPPWGWGAPPPPVWDGPLPAAWGPPPPPINYFGYNEQPVWDPGYNQWGFYFFGIW
                     IPLPF"
BASE COUNT          454 a          789 c          810 g          428 t
ORIGIN      
        1 acgtcgacga tcgagctggg taccagcatc gcggtggcgt tcgcgcggaa tccgatgacc
       61 gtggccaaca tcggctggga tctgcaggcc ttctcgcaag gccgcttcat cctgggcctc
      121 ggttcgcaga tcaagcctca catcgagaac cgattcagca tgccgtgggg ccggcccgta
      181 gaacgcatgc gcgaattcgt gctggcgctt ggtgagatct ggtcgtgctg gcaggcgggc
      241 acgaaacttg ctttcgaagg tgacttctac acccacaagc tgatgacgcc gatgttcacg
      301 cccgaaccgt tggcgcacgc gttgccgaag gtgttcgtcg ccgcagtggg tgaagcgatg
      361 accgagatgt gtggcgaggt cgccgacggc ctgctcgcgc acgcgtttac gacgaagcgc
      421 tatttcgacg aggtgacgac gccggccctg ctgcgcggca tggagcgctc cgggcgcaaa
      481 cgcagcgagt tcgagctgtc gtgtccgctg ttcgtggtga cggggaacga cgacgcggag
      541 ttgacgacca atgccgttgg cacgcgcaaa cagatagcct tctacgcctc gacgccggcc
      601 tatcgcaggg ttctggaact gcacggctgg ggagacctgc agaccgaact gcaccggatg
      661 tcgaaggagg gccaatggga tgcgatgggt tcgctcatcg atgacgacat cctcaacgag
      721 ttcgcggtgg tggcgccgtt gaacgacgtc gcgcaggtca tccgtgaccg gtgcgacggc
      781 gtcatcgacc gtgtcctggt gggttttccc gcctcggtcg acgacgccgc cgtcgccacc
      841 gtgctcgacg agatgaggag ccgcgcgtga gtacgcccat catagacgaa gccgccaagg
      901 tcttcgccac cccgaaggcg tacaccgacg agcggaaact ccattcggcg ctgacgcatc
      961 tgcgcgcgga cgccccggtg tcatgggtcg atgtgccgtc atacgaaccc ttttgggcca
     1021 tcaccaagca tgccgacatc atggacatcg agcgggcgaa ccacgtgttc acgaactacc
     1081 cccggccggt gctggtcacg gccgaggatg acgagcggca ggcggccgtc ggcgtccgca
     1141 ccttgatcca catggatgat ccgcagcatc gcggcgtgcg ggcgatcggc gcggactggt
     1201 tccggccgaa ggcgatgcga gcactgaaga atcgcgcgga cgaactggcc aaggtctacg
     1261 tcgacaacat ggcggcgatc gggtccgagt gcgacttcgt ccagcaggtg gccgtgaact
     1321 acccgctgta catgatcatg tcgcttctcg gcgtgcccga atcggacttc ccgctgatgc
     1381 tgaagctcac ccaggagctg ttcggcagcg atgacgacga gttcaagcgc ggcaccgatg
     1441 gcggagatca gatgtctgcg ctgctcgaga tgttcgaata cttcaccgcg ttgaccgcat
     1501 cgcgacggga agaccccact gaggatctgg cttccgcgat tgccaccgcc acgatcgacg
     1561 gtgagccact gaccgacatc gacaccatct cgtactacgc gatcatcgcg gccgcaggac
     1621 acgacaccac cagcgcgacc atctcgggcg gcatgctggc gctgatcgag aatcccgatc
     1681 agcgtcgaat gctgaccgcc gacctggacc tgatgccgct ggccacggag gagatgatcc
     1741 gctgggtcac ccccgtcaag gcgttcatgc ggaccgccac cgaggacgcc actgttcgtg
     1801 gagtgccgat cgcagccgga caatcagttc tgctgtccta cgtctcgggt aaccgcgacg
     1861 aagacgtctt cgacgatccg ttccgcttcg acgtcacgcg agatcccaac aagcacatcg
     1921 ccttcggcta cggagtgcac ttctgcctcg gcgcggcgct ggcccgaatg gaggtgggca
     1981 gcttcttctc cgagctgcta ccgcgactcg agtccgtcga actcgcagga gacccgcagc
     2041 acgtggcgac gacattcgtc ggcgggatca aacaccttcc gatccgttac tcgctgcgct
     2101 gaagcgttga cgaccgcccc gggattatcc gaggcggccg tctcgccagg ttgtctgtca
     2161 gaacgggagt ggaatccaga tcccgaagaa atagaaaccc cattggttgt agccgggatc
     2221 ccataccggc tgctcgttgt agccgaagta gttgatcggc ggcggcggag ggccccacgc
     2281 ggcaggcagc ggaccatccc acaccggcgg cggcggcgcg ccccagcccc acggaggcaa
     2341 atcgttgccc catggggcgt cacggaaccg cccgcgccag tcgttgtcct ggggattcca
     2401 tggcggtcgc caatcgtggc cgccggggcg gttgtaatcg ccgggtccgc ggttgaagtc
     2461 gccgggcggt ggaccgtcgg g
//
LOCUS       DASYLX010000851         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1036617,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000851 DASYLX010000000
VERSION     DASYLX010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036617"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGP27_19985"
     CDS             <1..877
                     /locus_tag="VGP27_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7853614.1"
                     /translation="APAVVESFGILDAFYPGRIDLGVGRSAKRRREAAKKPSSKPQPK
                     PEPPVEWREVDGVVVPPPFDVRTTITDKLRAQMSILQQPEAVSPDFADQVDDILAMLS
                     GCYRVRDYEVHAVPGERSRLTPWVFGSSKGQSAQVAGAKGLPFVASYHITPATALDAV
                     DAYRAAFRPSAMLSKPYVVVSADVVVADDTATAQHLASTYGHWVYSIRKGGGAVPYPD
                     PDVAEPLTVDQFEIVKDRTATQFVGNADEVAGKLEALQRVTGADELVVTSVAHRHADR
                     LHSHELIAKRWGLTG"
     gene            874..>2000
                     /locus_tag="VGP27_19990"
     CDS             874..>2000
                     /locus_tag="VGP27_19990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770302.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HEV7853615.1"
                     /translation="MTAVREGGQRKPIHLAAHFPGVNNTTVWSDPDSGSQVEFDSFVH
                     LARTAERGFFDFFFLAEGLRLREHRGRIHDLDVVGRPDTFTILAALAAVTDRLGLVGT
                     INTTFNEPFEVARQFATLDHLSDGRAGWNMVTSSDAFTGENFRRGGYLDHADRYKRAA
                     EFITVARAFWDSWGDDAVRDDVEAGVYVDPHRINTVQHQGSQFDVRGVATLPVSPQRH
                     PVLLQAGDSSDGRAFGAEYADALFTLHSALEAGQRYYADVKERAAQSGRNPDTLKVFP
                     AATFVLGDSSAEATERARHIRYQQVTGPTAIAMLEQVWQRDLSGYDPDGPLPDVDPVD
                     DPTITQGRVRHGDPKAIAASWRERAEADNLSIRDLVIAVTSR"
BASE COUNT          338 a          716 c          640 g          306 t
ORIGIN      
        1 cgcccctgcg gtcgtcgaga gtttcggcat cctcgacgcg ttctaccccg gccgcatcga
       61 cctaggtgtg ggcaggtcgg cgaagcggcg tcgcgaggcg gccaagaagc cctcctcgaa
      121 gccgcagccg aagcccgaac cgcccgttga gtggcgcgag gtcgacggcg tggtcgtgcc
      181 gccgcctttc gacgtgcgca ccaccatcac cgacaaactt cgcgcgcaga tgtcgatact
      241 gcagcagccc gaagcggtct cgcccgactt cgccgatcag gtcgacgaca tcctcgccat
      301 gctgagcggc tgctaccgcg tcagagacta cgaggtgcac gccgtgcccg gcgagcgaag
      361 tcgcttgacg ccgtgggtct tcggcagcag caagggacag agcgcgcagg tcgcgggagc
      421 caaggggctg cctttcgtcg ccagctacca catcacgcca gccaccgccc tggatgcggt
      481 cgacgcctat cgggccgcct tcaggccgtc agcgatgctg tcgaagccct acgtggtcgt
      541 ctcggccgac gtggtcgtcg ccgatgacac cgccacggcg caacacctgg catccaccta
      601 cggccactgg gtctactcca tccgcaaggg cggcggtgcc gttccctacc cggatccaga
      661 cgtcgccgag ccgctgacag tcgatcagtt cgagatcgtg aaggaccgca ccgcaacgca
      721 attcgtcggc aacgccgatg aggtggccgg caagctggaa gctctgcagc gggtgaccgg
      781 tgccgacgaa ctggtcgtca cctccgtggc ccaccggcac gccgatcggc tgcactcaca
      841 cgagctgatc gccaagcggt gggggttgac cggatgacgg ccgttcgaga aggcgggcag
      901 cgcaagccca ttcacctggc cgcccacttt cccggtgtca acaacaccac cgtctggtcg
      961 gatcccgact cagggagtca ggtcgagttc gactccttcg tgcacctggc ccgcaccgcc
     1021 gagcgcggct tcttcgactt cttcttcctc gccgagggcc tgcgcctgcg cgaacaccgc
     1081 ggccgcatcc acgacctcga cgtcgtcggc aggcccgaca ccttcacgat cctggcggcg
     1141 ctggcggcgg tcaccgaccg gctcggcctg gtcggcacca taaacaccac gttcaacgaa
     1201 cctttcgagg tggcaaggca attcgccacg ctcgatcacc tttctgacgg ccgggccggc
     1261 tggaacatgg tgacgtcgtc cgacgccttc accggggaga acttccggcg cggcggctac
     1321 ctcgatcacg ccgaccggta caagcgcgcc gccgagttca tcaccgtcgc gcgcgcattc
     1381 tgggacagct ggggcgacga cgcagtgcgg gatgacgtgg aagcgggggt ctatgtcgac
     1441 ccgcaccgca tcaacacggt ccagcatcag ggatcgcagt tcgatgttcg tggcgtggca
     1501 acgcttcccg tcagcccgca gcgacatccg gtgcttttgc aggcgggcga ctcgtcggac
     1561 ggcagggcct tcggcgccga atacgccgac gccctgttca cgttgcactc tgcgctcgag
     1621 gccggccagc ggtactacgc cgacgtcaag gagcgcgccg cacagtcggg ccgaaacccc
     1681 gacacgttga aggtctttcc cgctgcgacc ttcgtgctcg gcgacagctc cgcggaggcg
     1741 accgagcgtg ccaggcacat ccgctatcag caggtgaccg gcccgacggc gatcgccatg
     1801 ctggagcagg tttggcagcg cgacctttcc ggctacgacc ccgacggccc gctgcctgac
     1861 gtcgacccgg tcgacgaccc gaccatcacc cagggccggg tgcgccacgg tgacccgaaa
     1921 gccatcgccg caagctggcg ggagcgcgca gaggccgaca acctcagcat ccgtgacctg
     1981 gtgatcgccg tgaccagccg
//
LOCUS       DASYLX010000852         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_378182,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000852 DASYLX010000000
VERSION     DASYLX010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378182"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="fdxA"
                     /locus_tag="VGP27_19995"
     CDS             <1..269
                     /gene="fdxA"
                     /locus_tag="VGP27_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7853616.1"
                     /translation="ECPVDCIYEGARMLYIHPDECVDCGACEPVCPVEAIYYEDDVPD
                     QWSGYTQINADFFAELGSPGGASKVGQTDNDPQSVKDLPPQGED"
     gene            328..1416
                     /gene="dapC"
                     /locus_tag="VGP27_20000"
     CDS             328..1416
                     /gene="dapC"
                     /locus_tag="VGP27_20000"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEV7853617.1"
                     /translation="MSAGLPVFPWDTLADATAMARSHTDGIVDLSVGTPVDPVAPLIR
                     DALAAASPAPGYPATAGTPALRASAVTALNRRYGVTDLAEDAVLPAIGTKELIAWLPT
                     LLGLGPEDVVAVPELAYPTYEVGARLAGAQVLLADSLTQLGPLSPALIYLNSPSNPTG
                     AVLGADHLRKVVGWARERGVAVASDECYLGLGWDAEPVSLLHPSVCDGDHTGLLAIHS
                     LSKTSSLAGYRAGFVAGDRALIAELLAVRKHAGMMMPTPVQAAMVAALDDDAHEREQR
                     ERYQRRRDALLPAVRSAGLDVDHSEAGLYIWATRGEPCRDTVRWLAQRGILVAPGEFY
                     GPRGSQHVRIALTATDERIAAAVERLTA"
     gene            complement(1516..1737)
                     /locus_tag="VGP27_20005"
     CDS             complement(1516..1737)
                     /locus_tag="VGP27_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853618.1"
                     /translation="MHYYRQRAGTPLAGGERSEGEKHRNAKLTSANVRELRSLYADGA
                     TLRQLGEKFGVSNVSIYNAVAGKTWRHLQ"
     gene            2096..>2230
                     /locus_tag="VGP27_20010"
     CDS             2096..>2230
                     /locus_tag="VGP27_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853619.1"
                     /translation="MSDYSVEAVDRLPFTTPEKSARYRTEDYRGAAGLNWYATDPTLQ
                     A"
BASE COUNT          379 a          774 c          700 g          377 t
ORIGIN      
        1 aagaatgccc tgtcgactgc atctacgagg gcgcacgcat gctgtacatc caccccgacg
       61 aatgtgtgga ctgcggcgcg tgtgaacccg tctgccccgt cgaggccatc tactacgaag
      121 acgatgtgcc cgatcagtgg agcggataca cccagatcaa cgccgacttc ttcgccgaac
      181 tcggctcacc cggtggcgcg tcgaaggtcg gccagaccga caacgacccg cagtcggtca
      241 aggacctgcc gcctcagggt gaggactgac accgcggacg cgccgagcga tacggctggc
      301 gtaacacccg gcggcgttcg ccgcggcatc tctgcggggc tgcccgtatt cccttgggac
      361 accctcgccg acgcgacggc catggcgcgc tcgcacaccg acggcatcgt cgacctgtcc
      421 gtcggcacgc cggtcgatcc ggtcgcaccg ctgatccgcg acgcgttggc agcggcaagc
      481 cccgctccgg gttatccggc cacagcaggc acccccgcgt tgcgcgcgtc ggcggtcacg
      541 gcgttgaacc gccgctacgg cgtaaccgac ctcgccgagg acgccgtgct acccgcgatc
      601 ggcaccaagg aactcatcgc gtggctgccg acgctgctcg gcctggggcc agaggatgtg
      661 gtggcggtgc ccgaactggc ctatcccact tatgaagtcg gcgcgcgact ggcgggcgcg
      721 caggtccttc tcgccgattc gctgacccag ctcggtccgc tgtcgcctgc cctgatctat
      781 ctcaactcgc cgagcaatcc cacgggagcc gtcctgggcg ccgaccacct ccgcaaggtc
      841 gtcgggtggg cgcgcgagcg cggcgtggcc gttgcgtccg acgagtgcta tctgggtctt
      901 ggctgggacg ccgaacccgt gtcgctgctg catccctcgg tctgcgacgg cgatcacacc
      961 ggcctgctgg ccattcactc cctgtccaag acgtcctcgc ttgccggcta ccgcgccggc
     1021 ttcgtcgcgg gcgaccgcgc actgatcgcc gaactgcttg cggtgcgcaa gcatgcaggg
     1081 atgatgatgc cgacgcccgt gcaggccgcg atggtcgccg cgctagacga tgacgcgcac
     1141 gaacgggagc agcgtgagcg ataccagcgc cgccgcgatg cgctcctgcc tgcagtgcga
     1201 tcagcaggcc ttgacgtcga ccactcagag gccgggttgt acatctgggc cacgcgaggc
     1261 gaaccgtgtc gcgacaccgt cagatggctt gcgcagcgtg ggattctcgt cgcgccgggc
     1321 gagttctacg ggccacgtgg ttcgcagcac gtgcgaatcg ccctcaccgc caccgacgaa
     1381 cggatcgccg ccgccgtcga gcgactgact gcctgacggg tattggccca tcggcgcgga
     1441 gccggtgtgg accaccgcgg aggcacagcc aattccgatc cggcaacggg cctgcggcgc
     1501 ggcggttgac gccggctatt ggagatggcg ccaggtcttg ccggccaccg cgttgtagat
     1561 cgacacattc gacacgccga atttctcccc gagttgccgc aaagtggcgc cgtcggcata
     1621 aagcgagcgt aattccctca cgttggcgct cgtcagcttt gcgttgcgat gcttttcgcc
     1681 ttcagaccgc tcaccgccgg ccaggggcgt gccggcccgc tgtcggtagt aatgcattcc
     1741 gcaaagtccc cgcgctctgt atttacggtc gcatccgtct accgtacaga actcggggag
     1801 cccctttggc ctccccatac gactaaatat tacgaggagt atgggggtgt ttagcaaggg
     1861 ctagcccgca attcttcgaa atcaagagca acgacccggt ttggttgctt aacgcaaaca
     1921 aaggttcaat ctggccgcat taatccgggc aatagtttcg cgccaccgct gtcaatacaa
     1981 accgcggcta aaatgcggcc gataacgcac gcatttctgc tccgccaccg agtcgacgac
     2041 gactgcgggc tgccggtgct tcacccgacg ccgaacctgc aataccgtcg gcatcatgag
     2101 cgattacagc gtcgaggccg tcgaccggct gcccttcacg acgccggaga agtccgcgcg
     2161 ctaccgcacc gaggactacc ggggagcggc cggcctcaat tggtacgcca ccgaccccac
     2221 gctgcaggcc
//
LOCUS       DASYLX010000853         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3669203,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000853 DASYLX010000000
VERSION     DASYLX010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669203"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGP27_20015"
     CDS             <1..649
                     /locus_tag="VGP27_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853620.1"
                     /translation="NNDAIVVGLSRVGRIISSAALLLTITLLSFANGLSFMKMFGIGT
                     ALAIVIDATLIRGVVVPAFLRVADDLNWWAPRPLKWIHARIGLSEAPHDIESPPPALA
                     QEEDTQRLRPMRGAPEPDTIRRPRPTPAQDDVTQRLTPIRTPEPDTIRHPRPTPQHDS
                     TPQQEEDTQRLRPMGAPEPDTIRHPKPRPQQDETTQKLSPSRAPKPRPSGTQRPR"
     gene            831..2000
                     /locus_tag="VGP27_20020"
     CDS             831..2000
                     /locus_tag="VGP27_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853621.1"
                     /translation="MADDYARHALSQRASFEAIHPAVMAAAERLASEPVLCVADLGAA
                     DGVNSHGLIRELVAQRAGRPLIYAFVDLPTNAWRVAAAQLRNAFGAAENQGVLVVLPT
                     PAESGPGVEDEGTGANYCSPEAHGEACRRALGRDPRPATVVSMAGIPLQQAPSLPRGT
                     VHIAVTGTTMHWVAEAAGLASSGSVFPGYPDHVDDDERRGWRVAAERQWEHMLEMRAV
                     ELAPGGWFIGAIPASPDPCPDRMGVYVEIVGDMNLLLGEWRRAGRIGGATVAAAVVPV
                     WARTLDELRAPFEAGGGRFAGLELESIELFRLDNPYRHDDPAVFAREYVKSVTAWGGP
                     LLLRAFALEGEAGAAGLLADFLAELEQRVAQAPDRYRWDYVEALVICRQTSGAGV"
     gene            2002..2088
                     /locus_tag="VGP27_20025"
                     /pseudo
     CDS             2002..2088
                     /locus_tag="VGP27_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(2138..2287)
                     /locus_tag="VGP27_20030"
     CDS             complement(2138..2287)
                     /locus_tag="VGP27_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853622.1"
                     /translation="MSTPDHVENTGDNLRHAAEVQKQAQDDQSEEHVPPDQQASAGAP
                     PDFNT"
     gene            2409..2834
                     /locus_tag="VGP27_20035"
     CDS             2409..2834
                     /locus_tag="VGP27_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853623.1"
                     /translation="MAATRSVTLSALGAWVIKCNPHKTPIEPMRAAGEVKSSWCVADN
                     YRSRLIRPEHRVLFWVSAHPQRGLWGAGRVIGNVAGGDGQLRMPVHIRLFTEPVTAAQ
                     LFSLPELRSMEAFRSPQHSNPSWVSTAELALIDPLLPST"
     gene            complement(3109..>4048)
                     /locus_tag="VGP27_20040"
     CDS             complement(3109..>4048)
                     /locus_tag="VGP27_20040"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853624.1"
                     /translation="FALGGQWGGASLLLTENSPRSRRGFYGSFVQVGAQLGLIGGLSG
                     YLVLSLAFSDQQMMSWGWRIPFLSGIVMVALGFYIHRAIEETPIFRRLQAQGVSRQTG
                     ETRPETAPILRVIKTHWRTILLAGGAFVLPNAVAFIIVSGVLNYAVQTLGLRQGAVLG
                     VILAACVCPLFCIPYFAHLSDRYGRPRLFLIGALGVAIWAWPMFALIDTANLWLVLLA
                     LVVCFIGHSMMFGPQVALYTELFPTDLRYSGASLGYQVGTVFGGGLAPFVTASLLAAT
                     HTSWSVAGYIDVLAVISLTSIVLLRQQANAGAKELT"
BASE COUNT          739 a         1367 c         1292 g          650 t
ORIGIN      
        1 gaacaacgac gccatcgtcg tcggactaag ccgggtaggc aggatcatca gtagcgccgc
       61 actcctgctc accatcacat tgttgtcttt cgccaacgga ctgtccttca tgaagatgtt
      121 cggcatcggc acggcgttgg cgatagtcat cgatgccacc ttgatccgcg gtgtcgtggt
      181 gcccgcgttt ctgcgcgtcg ccgacgatct caactggtgg gcgccgcggc cgctgaagtg
      241 gatacacgcc cgcattggat tgagcgaagc cccgcacgac atcgagagcc caccgccggc
      301 gcttgcgcaa gaggaggaca cacagcggct ccgaccgatg cggggggcac ccgagcccga
      361 caccatccgg cgcccaaggc cgacacccgc acaagacgac gtgacacagc ggctcacacc
      421 gatccgcaca cccgagccgg acaccatccg gcaccccagg cccacacctc aacacgactc
      481 cacacctcaa caagaggaag acacacagcg actcagaccc atgggtgcgc cagagcccga
      541 caccatccgg caccccaagc ccagacctca acaagacgaa acgacgcaaa aactctcgcc
      601 cagccgcgca cccaaacctc gcccgtccgg cacgcaacgt ccgcggtgac gggcaatgtc
      661 gcgaccctcc ccggtcgcct tccaaacgaa cgcgcctctc gcgagtcgtt ttttgcagac
      721 gccgtccggc acgcctcccc tgtgggacga taagcgcgcg ccgtccggcc cgcggcggcg
      781 ctgtgcggga ggtaagcgcc acgccgtcac gtcgccccga gctcacgagc atggcggacg
      841 actacgcgcg gcacgccctc tcgcagcgcg catccttcga ggcgatccac ccggccgtga
      901 tggccgccgc ggagcggctc gcgagcgagc ccgtcctctg cgtggccgac ctgggcgccg
      961 ccgacggcgt gaactcacac ggcctcatcc gcgaactcgt ggcgcagcgc gccggccgac
     1021 cgctgatcta tgcgttcgtt gaccttccga ccaacgcgtg gcgggtcgcc gctgctcagc
     1081 tgcggaacgc cttcggcgcg gcagagaacc agggcgtgct ggttgttcta cctacgccgg
     1141 ccgagtccgg acccggggtc gaggacgagg gaacgggcgc taactattgc tcaccggagg
     1201 cgcacggcga ggcctgtcgc cgagcactcg gtcgcgaccc gcgtcccgcc acggtcgtct
     1261 cgatggccgg aatcccgttg cagcaggcgc catcccttcc acgcggcaca gtgcacatcg
     1321 cggtgaccgg gacgaccatg cactgggtgg ccgaagccgc gggtctggcc tcgagcggct
     1381 cggtcttccc cggctacccg gaccacgtgg atgacgacga gcgacgcggc tggcgtgtcg
     1441 ccgcggagcg gcagtgggag cacatgctcg agatgcgcgc agtggagctg gcgccgggag
     1501 ggtggttcat cggcgccatc ccggcctccc ccgatccatg cccggacagg atgggcgtct
     1561 acgtcgagat cgtcggcgac atgaacctgc tgctcggcga gtggcgccgc gcaggacgca
     1621 tcggcggcgc caccgtcgcc gcggccgtcg tcccggtctg ggcgcgcaca ctcgacgagt
     1681 tgcgggctcc cttcgaggcg ggcggcggca ggttcgccgg cctcgagttg gagagcatcg
     1741 agcttttccg actcgacaac ccgtaccgcc acgacgatcc cgcagtgttc gcgagggaat
     1801 acgtcaagag cgtcaccgct tggggaggtc ctctgctcct gcgtgccttc gcgctcgagg
     1861 gcgaggccgg cgccgccggt ctgctcgccg acttcctcgc agagctcgag cagcgcgtcg
     1921 cgcaggcccc cgatcgctat cgctgggact acgtcgaggc tctcgtcatc tgccgtcaaa
     1981 ccagcggcgc aggtgtctga cgcgtacaac ttcgacctcg agcttctcga gcaccggacg
     2041 ctggacggtc acatccaaga gctcatctac cgacccgcca tccactgatc ggcgcagctt
     2101 ggcacggcgc tcagatgaat gaagtcacgg cacgccgcta cgtgttgaag tcaggcggcg
     2161 cgccggcgga tgcctgttgg tcgggcggca cgtgctcctc ggactggtca tcctgcgctt
     2221 gcttttgaac ttcggctgcg tgacgaagat tgtcgcctgt gttctctacg tggtccggcg
     2281 tgctcacgaa acctccttgg ggtcgccact cggcataccc accgcggacg tggctttacc
     2341 aagtcggatt cccattaccc tggatgcaga ttcgcgcacg acccggacgc gaggatggtg
     2401 ttcttttcgt ggccgccaca cgtagcgtga ccctttcagc actgggggca tgggtcatca
     2461 agtgcaatcc gcacaaaacg ccgatcgaac ccatgcgtgc ggctggtgag gtgaagtcgt
     2521 cctggtgcgt ggctgacaac taccggtcgc gactcatccg gccggaacac cgcgtgttgt
     2581 tctgggtttc ggcgcacccg caaaggggtc tctggggagc gggccgggtc atcggcaatg
     2641 tggcaggcgg ggacggtcag ctgcgtatgc ccgttcacat ccggctgttc accgagcccg
     2701 tgaccgcggc gcaactgttc tcattgccgg agctgcgctc aatggaagcg ttccgttcgc
     2761 cccagcactc caacccgtcc tgggtgagca ccgcagaact ggcgttgatc gatccgctgc
     2821 tgccgtcgac ctgaccagac agcgcgcccg tcagctgtgc gatcgtcgac cttgagctgt
     2881 tctccggccg tggcgatgaa gacgaccagc catttcgccg cttcggtcgt gctggtgttg
     2941 tcgcgcagaa aagcacacag ggctgcgacg tcgtcccggg ttccgagtct tcctgcgggt
     3001 atgggccgta tcagcaggtc cgttcaacgg tattccgctg ttgcgcagac gaattcgccg
     3061 aacagtccac atcaatcgag cagatctcgt tggccacgtt cccggttgct acgtgagttc
     3121 cttcgctccg gcattggctt gctgccgcaa cagcacgatc gaggtgaggc tgatgacggc
     3181 cagcacgtcg atgtagccgg cgaccgacca actggtgtgg gtagccgcga gcagggatgc
     3241 cgtcacgaat ggagcgagcc caccaccgaa cacggtccca acctgatacc cgagggatgc
     3301 cccggaatag cgcaggtcgg tcgggaacag ctcggtgtaa agcgccactt gcggcccgaa
     3361 catcatcgag tgcccgatga agcagacaac gagcgcgagc aatacaagcc agaggttggc
     3421 tgtgtcgatg agggcgaaca tcggccaggc ccatatcgca accccaagag caccgatcag
     3481 aaacagtcgt gggcggccgt accggtcgct gaggtgagcg aagtacggga tacaaaacaa
     3541 ggggcagacg caggcggcga ggatcacgcc gaggacggca ccttggcgga gtccgagagt
     3601 ctgcaccgcg tagttcagca cgcccgagac gatgatgaac gcgacggcat tcggcaggac
     3661 gaatgcgccc ccggcgagaa gaatggttcg ccagtgagtc ttgatcacgc gcaggatcgg
     3721 cgcggtctcg ggtcgcgtct ccccggtctg gcgtgagaca ccctgcgcct ggaggcggcg
     3781 gaatatcggg gtctcttcga tcgcacggtg aatatagaag cccagcgcga ccatgacgat
     3841 tccgctcaga aagggaatgc gccatcccca ggacatcatc tgctgatcag agaaggcaag
     3901 cgacaggacg agatagcccg atagtccgcc gatgagtccc agctgcgccc cgacctgtac
     3961 gaagctgccg tagaaccctc gacgactacg aggagagttc tcagtcaaca gcagcgacgc
     4021 tccgccccac tggccgccga gcgcgaag
//
LOCUS       DASYLX010000854         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_818384,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000854 DASYLX010000000
VERSION     DASYLX010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818384"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VGP27_20045"
     CDS             complement(<1..735)
                     /locus_tag="VGP27_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853625.1"
                     /translation="MAGPVGLPVIDTMIGFPHEGSAQYDFIRRQTKDRESKESFEFPV
                     EYMFKDVPKGLPTDDPVSLVLQQMDRFGIEKAMIGVSEGSAELALKTFPDRFIPSGAI
                     ADPNDVMGSVNAIRREYEQYGIAATSVFPAGTFPQVPIDDPKMYPIYATCVELGIPIF
                     VCAGVPGPRVPFTSQEVSRIDIVMFDFPELVFVTRHGCEPWEDLAVKLMLKWPNLYYS
                     TSAFAPKYYPKAIIDYANSRGADKVLY"
     gene            963..1556
                     /locus_tag="VGP27_20050"
     CDS             963..1556
                     /locus_tag="VGP27_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7853626.1"
                     /translation="MSLADMTSGQLARRARIIEAVTDLIGAVGADAVQMRDVASRSGV
                     ALATVYRYFSSKEHLLAAALQDWQKRLTRRILAAGGPADEGPLPGVLDYLRRAQRAFH
                     RNPEMTALMLQAMTSTAPEAKAAIDHMGRTNVEMFDRVLEGIAPEEIADVSFGLNAAL
                     TSALTAVLTGALTLEESLSRVEWVARTLIGATTHRTV"
     gene            complement(1541..>1792)
                     /locus_tag="VGP27_20055"
     CDS             complement(1541..>1792)
                     /locus_tag="VGP27_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853627.1"
                     /translation="VFAFTMMSMAVSELTVIGQVGTTIGLGLLFDTLVIRSFMTPSIA
                     ALMGKWFWWPMRVRDRPVPSPWPTQPTAQRESRIGQTVR"
BASE COUNT          342 a          539 c          587 g          324 t
ORIGIN      
        1 gtagagcact ttgtccgcgc cgcgtgagtt cgcgtagtcg atgatggcct tcgggtagta
       61 cttgggcgcg aacgccgatg tcgagtagta caggttcggc cacttcaaca tcagcttgac
      121 ggcgaggtcc tcccacggct cgcagccgtg gcgcgtgacg aacaccagct cagggaagtc
      181 gaacatgacg atgtcgattc gtgaaacctc ttgcgacgta aacggaacac gggggccggg
      241 tacgccggca cagacgaaga tggggatgcc gagttcgacg caggtggcat agatggggta
      301 catcttcgga tcgtcgatcg gcacctgggg gaacgtgccg gccgggaaca ccgacgtcgc
      361 cgcgatgccg tattgctcgt actcgcgccg gatggcgttt accgaaccca tcacgtcgtt
      421 cgggtcggcg atggcgccgg agggtatgaa gcggtcgggg aacgtcttca gcgccagctc
      481 ggcagagccc tcactgacgc cgatcatggc cttctcgatg ccgaaccgat ccatctgctg
      541 cagcaccagc gacaccggat catcggtcgg caagcccttg ggcacatcct tgaacatgta
      601 ctcgacgggg aattcgaagc tctctttgga ctcccggtcc ttggtctgcc tgcggatgaa
      661 gtcgtattgc gccgagccct cgtgagggaa gccgatcatg gtgtcgataa ccggcagtcc
      721 gacaggtccg gccatggcat cctccgatcg ggtgcgagcg cgacagcacc gtcgtcgctg
      781 tccgatcaat attgaaacaa ggttctactt atgtcaagat tgcgtgtagc gttctagctg
      841 cgataacagc ggccagggcg aactgttgcc gcatatatcg aacagactcc agaaacgctg
      901 ttctcgatgt tggaggtcgg cccgtcaaca caaaaagccc tgaccacact caggccgaga
      961 ccttgtcgct ggcggatatg accagcgggc agttggcgcg gcgagccagg atcatcgagg
     1021 ccgtgaccga cctgatcggc gccgtgggcg ccgatgcggt acagatgcgc gatgtggctt
     1081 cgcgttcagg ggtcgcactg gccaccgtct accgatactt cagctcgaag gagcatctgt
     1141 tggcggcggc gttgcaggac tggcagaagc gattgaccag gcgaattctg gcggcaggcg
     1201 gtccggctga cgaaggaccg ttgcccggcg tactggacta cctgcgacgc gctcagcggg
     1261 cctttcatcg caaccccgag atgaccgcgc tgatgcttca ggcgatgacg tcgaccgccc
     1321 ccgaggcgaa agcggcgatc gaccacatgg ggcgtacgaa cgtggagatg ttcgaccgcg
     1381 tgctggaagg cattgcgccg gaggagatcg ccgatgtgag cttcggtctc aatgccgcgt
     1441 tgaccagtgc cctgactgcg gtgcttaccg gcgcgctgac tctggaggag tcgctgagcc
     1501 gggtcgagtg ggtggcgcgc accttgatcg gtgcgacgac tcaccgaacg gtctgaccga
     1561 tccggctttc gcgctgcgcg gtcggctgcg tcggccatgg cgagggcacc ggccggtcgc
     1621 gcacccgcat cggccaccag aaccacttgc ccatcagcgc cgctatggac ggcgtcatga
     1681 acgaccgaat caccagggtg tcgaagagca ggccgaggcc gatcgtggtg cccacctgcc
     1741 cgatcaccgt caactcgctg accgccatcg acatcatggt gaacgcgaag ac
//
LOCUS       DASYLX010000855         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3782221,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000855 DASYLX010000000
VERSION     DASYLX010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3782221"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..1207
                     /locus_tag="VGP27_20060"
     CDS             524..1207
                     /locus_tag="VGP27_20060"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV7853628.1"
                     /translation="MTFEELGEDFGIGTTTAWEYAHGMAKFLAEIIGCPVESLQGQVA
                     GKICLVDGTLVPTCNWRHRRDLHSGHRKRYGVNVQIICDIHGRVNASSKAFPGSWHDK
                     HCFDEAGLAAILAGSGGLIGDTGYQGIDGITPFKENHARKLTDDHHRFNAQVAAIRFV
                     VEQAIAHIKNWRIMTTRYRGHLDRIDNVILAAIGLQALNDQLSDRSLSLDPPEKFGCR
                     SCRDRANAL"
     gene            1266..2663
                     /locus_tag="VGP27_20065"
     CDS             1266..2663
                     /locus_tag="VGP27_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV7853629.1"
                     /translation="MRLLHSAANTSARFDDPNLVSCAGLVAVMRLAQRCDLAGLVGEH
                     LKVTVGVGANAHLKVPAIVAGMIAGADCFDDLDVLRHGGMAELFGGVRAPSTLGSFLR
                     GLHWGNVRQLEVVSRRLLADLAVTTPVLADAQVMAHLDIDSCQRRVYGHAKQGAGFGA
                     AKVGGYTVRLRGLNPLLAAVSTPTCAPVLVGTRLRGGTANSARGAESFTAEAITTARA
                     AGATGLLIARMDSAFYGGAPIAACRRADVRFSVTARTDPKIARAIAGIGEDAWTPIKY
                     PHAIFDEDEQRWISDAEVAEVPYTAFAGNKAHAVTARLIVRRVRRLAPPRQGELLPAW
                     RHHAIFTDSPLAMLAAETDHRDHAIIEQLIADLIDGPLAHLPSGHFAANAAWLTCAGI
                     AHNLLRAAATLASRRHGRARSSTLRRHLINVAARLAHRGRDQLILHLPQDWPWQDAFN
                     GLFDATHRAPPARAA"
BASE COUNT          608 a         1041 c          857 g          485 t
ORIGIN      
        1 gtcaaacaca agtttcacgg cgactggaac tacaccataa agaagtcgat ggacaaactc
       61 gatcaagaaa gtgagtaatt taaataccgg cccttaccgc agccaaccgc ccacttcacg
      121 agattggtca tacatcggaa ttgcaccaag ttgttccgga aggccgtcac gagggtagtc
      181 ccaattcgac cgcacgatgg acagataacc tgaagtgggt gccagtcttc cggttttacc
      241 gaaccattta cttcatagaa gtttttcgga aaccctctta atggggtcgg cgcgcctacg
      301 tagaatatgg gcccttcttg catagcatgg atctgccaga aacacacaac gcaagaaggg
      361 cagcccctag tgtaccgtct ccccggattg agttcgcgcg cggccgaatt ggtagtcaag
      421 tgggcgaccg aggaatttcc cgactggaca ccgaaagtcg ggagaccacc gaaagtcggg
      481 agaccaccga aactcgatgt gcctgtgctg gctgcgacgg aacatgacct tcgaagagct
      541 aggtgaagat ttcggtatcg gtaccacgac cgcctgggag tacgcccatg gaatggccaa
      601 gttcctcgcc gagataatcg gttgccccgt ggaatcactg caaggccaag tcgccgggaa
      661 aatctgcctc gtcgacggca ccctggtacc gacatgcaac tggcgccacc gccgtgacct
      721 gcactccggg caccgcaaac gctacggcgt caacgtccaa atcatctgcg acatccacgg
      781 acgcgtcaat gccagcagta aagcattccc cggcagctgg cacgacaaac actgcttcga
      841 cgaagcagga ttggcagcta ttctcgccgg ctccggtgga ctgatcggcg ataccggata
      901 ccagggcatc gacggcatca cccccttcaa agaaaatcac gcccgaaagc tcaccgacga
      961 ccatcaccgg ttcaacgcgc aagtcgccgc aatacgcttc gtcgtagaac aagccatcgc
     1021 acacatcaag aactggagaa tcatgaccac ccgctaccgc ggccacctcg accgcatcga
     1081 caacgtcatc ctcgccgcga tcggtcttca agcactcaac gaccaactct ccgaccgatc
     1141 cctcagcctc gatccaccgg agaaattcgg gtgtcgatcg tgtcgtgaca gggcaaatgc
     1201 cctctgagct gggatgatgg tgtttgcgaa gaccccatgc atccagaccg aagggcatct
     1261 gcgagatgcg attgttgcac agcgcggcga acacatcggc gcgattcgat gacccgaatc
     1321 tggtgtcgtg cgccgggttg gtagcggtga tgcgcctggc gcagcgctgt gacctcgccg
     1381 gcctggtggg tgagcacctg aaggtgactg tcggggttgg tgcgaacgcg cacttgaagg
     1441 tccccgcgat cgtggccggc atgatcgccg gagcggactg cttcgacgac ctcgacgtgc
     1501 tgcgccacgg tgggatggcc gagctgttcg gtggggtccg tgccccctcg accctggggt
     1561 cgtttttgcg cgggttgcac tggggcaacg tgcgccagtt ggaggtggtg tctcggcgtc
     1621 tgctcgcgga tctcgccgtg acaaccccgg tgctggccga cgcgcaggtg atggcgcatc
     1681 tggacatcga ctcctgccag cgccgggtct atggccacgc caagcagggc gcggggttcg
     1741 gcgcagcgaa ggtcggtggc tacacggtgc ggctgcgtgg gctcaacccg ttgctcgctg
     1801 cggtgtccac tccgacctgc gcgccggtgc tggtcggtac ccgactgcgt ggcgggaccg
     1861 cgaactctgc gcgcggtgcc gagtcgttta ccgctgaggc gatcaccacc gcccgcgctg
     1921 ccggggccac cgggctgctg atcgccagaa tggactccgc gttctacggc ggggcaccga
     1981 ttgcggcgtg tcgtcgcgcc gacgtgcggt tctccgtcac tgcccggacc gaccccaaaa
     2041 tcgcccgagc gattgcgggg atcggcgagg acgcctggac cccgatcaag tacccgcatg
     2101 cgatcttcga tgaggacgag cagcgctgga tctccgacgc cgaggtcgcc gaagtgccct
     2161 acaccgcctt cgccgggaac aaggcccatg cggtgaccgc taggctgatc gtgcgccggg
     2221 tccgccgcct cgccccaccc cgccaagggg aactgctgcc cgcctggcgc caccacgcga
     2281 tcttcaccga cagcccactg gcgatgctcg ccgccgagac cgaccaccgc gaccatgcca
     2341 tcatcgaaca actgatagcc gacctcatcg acggacccct cgcccaccta ccctcaggcc
     2401 atttcgctgc caacgccgcc tggctgacct gcgccgggat cgcccacaac ctgctgcgcg
     2461 ccgccgccac cctggcctcc cgccgccacg gccgggcccg cagctccacc ctgcgccgtc
     2521 acctgatcaa cgtcgcggcc cggctcgcgc accgcggacg cgaccagctg atcctgcacc
     2581 taccccaaga ctggccctgg caagacgcct tcaacggact cttcgatgcc acccaccgcg
     2641 ccccacctgc ccgcgcggcc tgaccgccat ctccactcca gcacaccccg acggcctccg
     2701 cggcccaccc cgcaccgacc gaccacaaca atccggacaa agccgcagaa ccgccagcgg
     2761 cggacccttc acaccccaaa acaacaaaac gatcaccgac acaaagaaac gatcaccaac
     2821 aattagccgg tggatcgagg ctgagttcgc ataaccggcc ggccgctccc cacgtccgca
     2881 cgcgcccatc ggcgagcctg ccaacaacgc ccactacctg cgaccatgac acccagaaaa
     2941 acgatctagc cctgtagtac taggctgatc gtgcgccggg tccgccgcct g
//
LOCUS       DASYLX010000856         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3231610,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000856 DASYLX010000000
VERSION     DASYLX010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3231610"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1586)
                     /locus_tag="VGP27_20070"
     CDS             complement(<1..>1586)
                     /locus_tag="VGP27_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV7853630.1"
                     /translation="TREPHSGRVTHTSGLSVGYLRQSDDFAVGTTVREVIVGGRPDHI
                     WAAEPETRAVVGHLLAEVELDSGVADLSGGERRRVALAAILIAGHDVLVLDEPTNHLD
                     VEVIGWLAQHLSGQRSNALVVVSHDRWFLDAVTTKTWEVHNGTVDAYDGGYAAYVLAR
                     AERMRLAAGTEARRRNLMRKELAWLRRGPPARTSKPKFRIQAANELIANEPPPRDSLV
                     LQRFATTRLGKDVFDLHRLRLDVGNRVILDKADWSIGPGARIGLVGVNGTGKTSVLRL
                     LAGELQPAAGTIKRGTTLQIGYLSQALAEMTDAHRVIDAVENRRRVSELAGGREITAD
                     TLLKDFGFTGDKLTARIADLSGGERRRLQFLRILLDEPNVLLLDEPTNDLDIDTLNVI
                     EDYLDDWPGTLIVVTHDRYFLERVTDVTYALTGGGRCDLLPGGIEQYLAERVAAETAP
                     TRQEPQRTESASARKRRTGKEMARIEGQLTKVDERITAMHEAMADAAADHVRLGELNA
                     ELNELLTLKESLEEAWLAAA"
BASE COUNT          235 a          537 c          521 g          293 t
ORIGIN      
        1 cggcggcggc caaccaggct tcttcgagcg attccttgag tgtcagcagc tcgttcaatt
       61 cggcgttgag ctcacccagc cggacgtgat cggctgccgc atcggccatc gcttcgtgca
      121 tggcggtgat gcgctcgtcc accttggtca gttgaccttc gatgcgggcc atctccttgc
      181 cggtgcgtcg ctttcgcgcg gatgccgatt cggtgcgctg cggctcctgc cgcgtgggtg
      241 cggtctcagc ggcgacccgc tcggcgagat attgctcgat gccgccgggc aacaggtcgc
      301 atcgccctcc gccggtgagg gcgtacgtca cgtcggtgac gcgctcgagg aaataccggt
      361 cgtgagtgac gacgatcagc gtgcccggcc agtcgtccag gtaatcctcg atgacgttga
      421 gcgtgtcgat gtcgaggtcg ttggtcggtt cgtcgagcag cagcacattc ggctcgtcga
      481 gcaggatgcg caggaattgc agccggcgcc gctcgccgcc cgacaggtcg gcaatccgcg
      541 cggttagctt gtcgccggtg aaaccgaagt ccttcagcaa ggtgtcagcg gtgatttccc
      601 ggccgcccgc caattcgctg acgcggcgcc ggttttcgac ggcatcgatc acccggtgcg
      661 cgtcggtcat ctctgccagc gcctggctga ggtagccgat ctgcagcgtg gtgccgcgct
      721 tgatcgtgcc ggctgcgggc tgcagttcac cggcgagcag ccgcaacacc gatgtcttgc
      781 cggtgccgtt gacaccgacc agcccgatcc gcgcacccgg gccgatggac cagtcggcct
      841 tgtcgaggat gacgcggtta ccgacgtcga gccgcagccg gtgcagatcg aatacgtctt
      901 tgcccagccg cgtcgtcgcg aagcgctgca gcacaagcga atcacgcggt ggcggctcgt
      961 tcgcaatcag ctcgttggcg gcctggatgc ggaacttcgg tttcgacgtc ctggccggcg
     1021 gaccgcgccg cagccacgcc aactccttgc gcatcaaatt tctgcggcgc gcctcagtgc
     1081 ccgcagccag tcgcatccgt tccgcccggg ccagcacata ggcggcgtag ccaccgtcgt
     1141 aggcgtccac ggtgccgttg tgcacttccc acgtcttggt ggtgaccgca tcgagaaacc
     1201 accggtcgtg gctgaccacc accagtgcat tggacctctg ccccgacaag tgctgggcca
     1261 gccagccgat cacctcgaca tcgagatggt tggtcggctc atcgagcacc agcacgtcat
     1321 gcccggcgat cagaattgcg gccagcgcca cccggcgccg ctcaccgccg ctgaggtcgg
     1381 ccacgccgct gtcgagttcg acttcggcca gcaagtgccc gacgaccgca cgtgtctcgg
     1441 gttcggccgc ccagatgtgg tcgggcctgc cgccgacgat gacctcgcgg acggtggtgc
     1501 cgacggcgaa gtcgtcgctt tggcgcaggt atcccaccga cagtcccgat gtgtgggtga
     1561 ctcgccccga atgcggctcg cgggta
//
LOCUS       DASYLX010000857         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2683384,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000857 DASYLX010000000
VERSION     DASYLX010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683384"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGP27_20075"
     CDS             complement(<1..398)
                     /locus_tag="VGP27_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEV7853631.1"
                     /translation="MVAAEDAPNFALRLGIQKDQVVQELGWDDDVDDDIRADIEDACG
                     GELLDEDADEVVDVVLLWWRDDDGDLVDRLMDAITPLADDGLIWVLTPKTGKPGHVMP
                     AEIAESAPTAGLMQTSSANLGDWSASRLVQP"
     gene            complement(480..1061)
                     /locus_tag="VGP27_20080"
     CDS             complement(480..1061)
                     /locus_tag="VGP27_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853632.1"
                     /translation="MQTGALTRSWRLRIVAQVVGGLAGLAMIIVGCSSVTEGSPKVNP
                     NDAPVYRASVSASSQESAASSSSKESERQKSSSTEAVHSSCEALSTSSVDSITAVNDY
                     VKAFNDSAPDAAAKAGPAIDSLNHSADLVAQSISDPLSPQLRDALNAWVDAARGVANA
                     IAGNYGPDDFNASVSKLNDAKTTALDLCDAAYR"
     gene            1196..>2473
                     /locus_tag="VGP27_20085"
     CDS             1196..>2473
                     /locus_tag="VGP27_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEV7853633.1"
                     /translation="MTTEFARQDLAQTSGTAAEPDRVRVIREGVASYLPDIDPEETTE
                     WLESFDQLLERQGPARARYLMLRLLERAGEQRVAIPSLTSTDYVNTIPTELEPWFPGD
                     EEIERRYRAWIRWNAAIMVHRAQRPGVGVGGHISTYASSAALYEVGFNHFFRGKSHPG
                     GGDQIYIQGHASPGIYARAFLEGRLTADQLDGFRQEHSHPGGGIPSYPHPRLMPDFWE
                     FPTVSMGLGPMNAIFQARFNHYLHDRGIKDTSNQHVWAFLGDGEMDEPESRGLIQVAA
                     NEALDNLTFVINCNLQRLDGPVRGNGKIIQELESFFRGAGWNVIKVVWGREWDALLHA
                     DRDGALVNLMNTTPDGDYQTYKANDGAYVRDHFFGRDPRTKALVDKMSDTEIWNLKRG
                     GHDYRKVYAAYRAATEHKGQPTVILAKTIKGYSL"
BASE COUNT          422 a          834 c          792 g          425 t
ORIGIN      
        1 ggctgcacca gcctgctggc gctccagtcg ccgaggttgg ccgacgacgt ctgcatcagc
       61 ccggccgtcg gcgccgattc ggcgatctcg gcgggcatca cgtggcccgg tttgccggtc
      121 ttgggagtca acacccagat caaaccgtca tcagccagcg gtgtgatggc gtccatcaac
      181 ctgtcgacca ggtcgccgtc gtcgtcgcgc caccacagca gcaccacgtc gacgacctcg
      241 tcggcgtcct cgtcgagcag ctcgccgccg cacgcgtcct cgatatcggc ccggatgtcg
      301 tcgtcgacgt cgtcgtccca gcccaattcc tgcacaacct gatctttttg gatgcccagc
      361 cgtagggcga agttcggggc gtcctccgcc gcgaccaccg tcggtcctcc ttcaactgct
      421 cgtagaatcg tcctatcgtc gcacgagccg catggttggc ggtgccgggc gctcaaggtt
      481 caacgatatg cggcgtcaca caggtccagc gccgtcgtct tggcgtcgtt caatttggac
      541 accgaggcgt tgaaatcgtc gggcccgtag ttgcccgcga tggcgttcgc gacgccccga
      601 gcagcgtcga cccaggcgtt gagcgcatcc cgcagctggg gcgacagcgg gtcgctgatg
      661 ctctgggcga cgaggtctgc gctgtggttg agggagtcga tggcaggacc ggccttcgcc
      721 gccgcatccg gagcactgtc attgaaagcc ttgacgtagt cgttgaccgc cgtgatcgag
      781 tccacgctgc tggtgctcag cgcctcgcac gagctgtgca ccgcctcggt ggacgacgac
      841 ttctggcgtt cggactcctt ggagctcgac gaggcggccg actcctggct cgacgccgaa
      901 accgatgccc ggtagaccgg tgcgtcgttc gggttcacct tgggtgagcc ctcggtgacg
      961 gagctgcatc cgacgatgat catggcgaga ccggccaggc cgccgaccac ctgagcgacg
     1021 atccgcagcc gccatgacct ggtaagcgcc cccgtctgca cagtctgcag acgttaccgg
     1081 tttggacggc tggtcggcgg ttcattccac tagccaccac cggcagtgca agtgcggcac
     1141 gatgggtgga cgataggggt tgtcgagacc tatacctgga gacgaggagt ggaagttgac
     1201 caccgagttc gcgcgccagg acctggccca aacttccggc accgcagccg aacccgaccg
     1261 ggtgcgcgtc atccgtgaag gcgttgcgag ctacctgccc gatatcgacc ccgaagagac
     1321 caccgagtgg ctggaatcct tcgatcagtt gctggaacgg cagggcccag ctcgggcgcg
     1381 ctatctgatg ctgcggctgc tcgagcgcgc aggcgagcag cgagtggcca tcccgtcgct
     1441 cacctcgacc gactacgtca acacgattcc gaccgaactg gagccgtggt tccccggcga
     1501 cgaggagatc gagcgtcgct accgcgcgtg gatccggtgg aacgcagcga tcatggtgca
     1561 ccgtgcgcag cggcccggag tcggtgtcgg cgggcacatt tcgacgtatg cctcctcggc
     1621 ggcgctctac gaggtgggct tcaaccactt cttccgaggc aagtcccacc cgggcggcgg
     1681 tgaccagatc tatatccagg gccacgcctc tcccggcatc tacgcacgcg cgttcctgga
     1741 gggtcggctc accgccgacc agctcgacgg cttccgccag gagcacagcc atcccggcgg
     1801 cggcatcccg tcgtatccgc atccgcggct gatgcccgac ttctgggagt tcccgacggt
     1861 gtcgatgggc ctcggcccga tgaacgcgat cttccaggcc cgtttcaacc attatctgca
     1921 cgaccgcggc atcaaggaca cctccaacca gcatgtctgg gcgttcctcg gcgacggcga
     1981 gatggacgag ccggagagcc gcgggctgat ccaggtggcc gccaacgagg cactggacaa
     2041 tctgaccttc gtgatcaact gcaacctgca gcgcctggac ggcccggtgc gcggcaacgg
     2101 caagatcatt caggagctcg agtcgttctt ccgcggcgcc ggctggaacg tcatcaaggt
     2161 ggtgtggggc cgtgagtggg acgcgctgct gcatgccgac cgcgacggcg ccctggtcaa
     2221 cttgatgaac accacccccg acggggatta ccagacctac aaggccaatg acggcgccta
     2281 cgtgcgcgac cacttcttcg gccgcgaccc gcgcaccaag gcgctggtcg ataagatgtc
     2341 ggacaccgag atctggaacc tcaagcgcgg cggccacgac taccgcaagg tctacgcggc
     2401 ctaccgcgcc gccaccgagc acaagggtca gccgacggtg attttggcca agaccatcaa
     2461 gggctattcg ctg
//
LOCUS       DASYLX010000858         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_929164,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000858 DASYLX010000000
VERSION     DASYLX010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929164"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGP27_20090"
     CDS             <1..613
                     /locus_tag="VGP27_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853634.1"
                     /translation="RLTRTEEDDVVRYVAPSALSRLQLLTGMVYANRPWRLVAGMSKV
                     MMAAFATGAVSLAYPTIWQLSATMGPWRLSAATILASAAMIAWLIVDHKLWERPRSAG
                     ARDRAVLYNASTVITLTIGVAIFHTGLFILLLLAAWWTLPPQLVAQNIGHPVGFRTLL
                     MMAWLVAAVATLGGALGSGMEDDDAVKAAAYGARQRQRFDNSN"
     gene            complement(663..1859)
                     /locus_tag="VGP27_20095"
     CDS             complement(663..1859)
                     /locus_tag="VGP27_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEV7853635.1"
                     /translation="MGAAAARRIIGVLVLVSPLEQVAFDIYTPALPRIAEHFAASNTL
                     VQNTVTAYVVGMALVVLPAGLIADAIGRKRVLLAGLGLMTLTSIGCALAVNLPMMIGL
                     RFLQGLGAGTCLLIAATVAADCFRDAKLVSVLGLLGAAWGSAPVVAPAIGGFISQFGS
                     WRLVFVVLTALTATVAILVALLLPETLQPERRSRVDLRAAAGVLGEALRHRAFVAFAL
                     MFGLVAAAQTVFGVVGPFLYQVGLGFSAATYGFIALGVGAANFLGAATCGALAQRTTT
                     RRLAIGGAAVLALAGVIMLASAQFVGLSALTITVGAAVALLSIGVLDPLTKGLAMGVF
                     SRNIGLITGLISTFCYGSIAVAMALMAWLPERSQAPLGWFYVAAAVGLVAVLMTTVKR
                     ASAASS"
     gene            complement(2037..>2266)
                     /locus_tag="VGP27_20100"
     CDS             complement(2037..>2266)
                     /locus_tag="VGP27_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(R)-hydratase"
                     /protein_id="HEV7853636.1"
                     /translation="AGFASAWLNDPGAVTRYAVRLSAPAIVSAAEGADIEFSGRIKSL
                     DPEKRTGVVLVGAKSNGRKIFGLATLDVRFR"
BASE COUNT          425 a          746 c          744 g          351 t
ORIGIN      
        1 gcggctgacg cggacagagg aggacgacgt tgtccgctac gtcgcgccgt cggccctgtc
       61 ccggctgcag cttctgacgg gcatggtgta cgccaaccgg ccgtggcgat tggttgccgg
      121 tatgtcgaag gtgatgatgg cggcgttcgc cactggggcg gtaagccttg cgtatccgac
      181 gatttggcag ttgtccgcca ctatgggccc ctggcggttg agcgcggcga cgattctggc
      241 gagcgcagcg atgatcgcgt ggctcatcgt tgaccacaaa ttgtgggagc ggccgcggtc
      301 ggccggcgcg cgtgaccgcg cggtgttgta caacgcatcc actgtcatca ccctgacaat
      361 cggcgtcgcc atttttcaca ccgggctgtt cattctgctg ctgctggcag cttggtggac
      421 gcttccgccg cagctggtcg ctcagaacat cggtcatccc gtcggattcc ggaccttgtt
      481 gatgatggcg tggttggtgg cggcagtcgc gacgttgggc ggcgccctgg gatcggggat
      541 ggaggacgac gacgcggtca aggcggccgc atacggggct cggcaacgcc agcgattcga
      601 caactcgaat tgacgcacgc ccccggcggt cagtcgccgc cgaacctcga agcgttgcgc
      661 ggctatgacg acgcagccga cgcccgcttc accgtggtca tcagcaccgc caccagcccg
      721 accgccgccg cgacgtagaa ccagccaagg ggtgcctgag accgctcggg cagccatgcc
      781 atcagcgcca tggccaccgc gatcgatccg tagcagaacg tgctgatcaa tccggtgatc
      841 aaaccgatgt tgcgggagaa cacgcccatc gcaaggccct tcgtcaacgg gtcgaggaca
      901 ccgatgctga gcagtgccac cgcggcgccg acggtgatcg tcagagcgga cagcccgacg
      961 aactgcgcgg aggccagcat gatcacaccg gccagcgcca gcaccgcggc tccgcctatg
     1021 gccaaccgcc gcgtagtggt ccgctgggcc aatgcgccac acgtcgccgc accgaggaag
     1081 ttggcggcgc ccacgccgag tgcgatgaaa ccgtatgtgg cagcggagaa tccgagtcca
     1141 acctggtaca agaaggggcc gacgacaccg aataccgtct gcgccgccgc caccaaaccg
     1201 aacatcagcg cgaacgcgac gaaggcgcgg tgccgcagcg cctcacccag aacgccggcc
     1261 gctgcgcgca gatcaacccg tgaacgacgt tctggctgga gggtttccgg caacaggagg
     1321 gccaccagga ttgcgaccgt tgcagtcaaa gccgtcagca ccacgaaaac cagtcgccac
     1381 gaaccgaact gcgatatgaa accgccgatg gccggcgcca ccaccggagc cgatccccac
     1441 gcagcgccga gcaagcccag aaccgaaacg agtttggcgt cgcgaaaaca atccgcggcg
     1501 acagtggcgg ctatcagcag acatgtcccc gcgccgagcc cttgcaggaa acgcagcccg
     1561 atcatcatcg gtaggttgac ggccagcgcg cagccgatac tcgtcagtgt catcaacccc
     1621 agtccggcca acagcacgcg cttgcgcccg attgcgtccg cgatcagtcc ggcgggcagc
     1681 accaccagcg ccatgcccac tacgtaggca gtgacggtgt tctgcaccag ggtgttggac
     1741 gccgcgaaat gctcggcgat cctgggcagc gcgggcgtgt agatgtcgaa ggccacctgc
     1801 tccaggggac tgaccagaac caggacgccg atgatgcgcc gggccgcggc agcgcccatc
     1861 tgggggctgc ttcgaaatgg cctggagagc agtgagattg gcatcgggtg ctccgtcttc
     1921 ggttggcacc tgcggtggtg cgacttcgaa attacggacg tcgagctatc gtgtcgtgct
     1981 tgtgcgcact agccgcgcaa gcagcgataa gcggccttgt gcgcattgtg ctcaggtcag
     2041 cggaagcgga cgtccagcgt cgcaaggccg aatatcttgc ggccgttgga cttcgcgccg
     2101 accaggacga cacccgtgcg cttttccggg tccagcgact tgattcggcc gctgaattcg
     2161 atgtccgcgc cctcggcggc cgacacgatc gcgggtgccg acagccgcac cgcgtagcgg
     2221 gtgaccgcac cgggatcgtt caaccacgcc gaggcgaatc cggcac
//
LOCUS       DASYLX010000859         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2573368,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000859 DASYLX010000000
VERSION     DASYLX010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2573368"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VGP27_20105"
     CDS             complement(<1..143)
                     /locus_tag="VGP27_20105"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV7853637.1"
                     /translation="MTLQRPAPATGRHVVIAPDKFKGSLTARQAARAMSLGVKRADPT
                     LTTV"
     gene            500..1351
                     /locus_tag="VGP27_20110"
     CDS             500..1351
                     /locus_tag="VGP27_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV7853638.1"
                     /translation="MMNIAYTVNCSILLTELPLLDRPRAARDAGFAAVEFWWPFQSPT
                     PTDAEVTAFTGAIQDAGVQLTGLNFAAGDMPAGDRGILSNPAMTQTFRESVDIAVDIA
                     GMLGTRAFNALYGNRIPQSTPAAQDYVGAENLAYAAQAADRIGATVLVEPVSGAPHYP
                     LKTAADAIHVIDRVQGEYGAANVRLLADLYHLHVNGDDVDAAIRHYCNRIGHVQIADA
                     PGRGEPGSGELPLNSYLQLLAALGYTGYVGLEYKATRSDTFDWLPPDQRGITAESARR
                     HAETRIQ"
     gene            1348..>1741
                     /locus_tag="VGP27_20115"
     CDS             1348..>1741
                     /locus_tag="VGP27_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7853639.1"
                     /translation="MSTIAFIGLGIMGGPMADHLVRAGHSVIGVDHSPERTARLVEAG
                     GKAADSIEQAVKGADVVAIMVPDSPDVHSVLLGDDGVFAHTQPGTLVIDFSSIRPDVT
                     AEFARVATERGIRIIDAPVSGGEPGAKNA"
BASE COUNT          313 a          596 c          532 g          300 t
ORIGIN      
        1 actgtcgtca atgtggggtc tgcgcgcttc acacccaggg acatggcgcg tgcggcttgc
       61 cgggcggtca gtgatccctt gaacttgtcg ggcgcaatca ccacatggcg acctgtcgca
      121 ggggcggggc gttgcaaggt catatccggt tcctctcagc tggaccgcac gacgtgacga
      181 cccaagcctg cgttccgtca tgcggaagag tcgtttcgca tcagctcgcc ccaaggctta
      241 cctactcgac cttgacaagc gagtggttca ggcgttcact ctactctata ttgatagctt
      301 gattccgcat tacggaatga gacagcatga acgcgctcta cctcgcactg gcgatccctc
      361 tggcgtgggc cgtcacgggt tatgccctcg tgtcggcgcc gtggcacaca gaagcggcgc
      421 aatcgaggtc ccgttctcgt tcgcgggggc ccgccaccgc gagcagtccc ctgtcgccga
      481 caggacagaa aggtatgccg tgatgaacat cgcctacacc gtcaactgct cgattctgct
      541 gaccgagctg ccactgctgg accggccgcg tgcggctcgc gacgccggct tcgcggcggt
      601 cgaattctgg tggcccttcc aatctccgac gcccaccgac gccgaagtca ccgcctttac
      661 cggcgccatc caggatgcgg gcgtgcagct gaccggtctc aacttcgccg ccggggatat
      721 gccggccggc gaccgcggga ttctctccaa tcccgcgatg acacagacat tccgcgaaag
      781 cgtcgacatc gccgtcgata tcgccgggat gctgggaacc cgcgccttca acgcgctcta
      841 cggcaatcgc atcccgcaat ccaccccggc ggctcaggac tatgtcggag ccgagaacct
      901 cgcctatgcc gcacaggcgg cggaccgcat cggggcgacg gtgctcgtcg agccggtgag
      961 cggcgcacca cactatccgc tcaagacggc cgccgacgcc atccacgtca tcgatcgggt
     1021 gcagggggaa tacggcgcgg ccaacgtgcg actgctcgcc gacctctatc acctccatgt
     1081 caacggcgac gatgtggacg ccgcaattcg ccactactgc aaccggatcg gccacgtaca
     1141 gattgccgat gcaccggggc gtggtgagcc cggaagcggt gaactgcccc tgaacagcta
     1201 cctccagctg ctcgccgcgc tgggctacac gggatacgtc ggcctcgaat acaaggcgac
     1261 gagatccgac accttcgact ggcttccccc agaccagcgc ggtatcaccg cggaatcagc
     1321 acgtcgacac gccgaaacga ggatccaatg agcaccatcg ctttcatcgg gctgggcatc
     1381 atgggcggcc cgatggccga ccatctagtc cgagccggcc attccgtcat tggcgtggac
     1441 cactcaccag aacgcacggc tcgtttggtc gaagcgggag gcaaggccgc cgactcgatc
     1501 gaacaggcgg tcaagggtgc cgacgtcgtg gcgatcatgg tgcccgactc tcccgacgtg
     1561 catagtgtgc tgctgggcga tgacggtgtt ttcgcccata cccagcccgg cacgctggtg
     1621 atcgacttct cttccatccg gccggacgtc accgcggagt tcgcccgcgt cgccaccgag
     1681 cgcgggatcc ggatcatcga cgcgccggtg tccggcggtg agccgggcgc caagaacgcc
     1741 a
//
LOCUS       DASYLX010000860         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2243654,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000860 DASYLX010000000
VERSION     DASYLX010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243654"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VGP27_20120"
     CDS             <1..1017
                     /locus_tag="VGP27_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEV7853640.1"
                     /translation="EAMAEHALVPVTFAEKEGLALINGTDGMLGQLVLALTDLDSLFR
                     LADIAAAMSVEGQLGTDRVFADDLQRLRPHPGQALSAANITRMLAGSAIVASHRGDDC
                     TRVQDAYSLRCSPQVAGAARDTAEHARAVAERELASVIDNPVITEDGRVESNGNFHGA
                     PVAYVLDFLSIVVADLASISERRTDRFLDVARNHGLNPFLADDPGVDSGHMITQYTQA
                     AIVSELKRLAAPASVDSIPSSAMQEDHVSMGWAAARKLRRGVDGLTQVLAIEVLNAAR
                     GIEMRLPLTPAPGTAAVIAAVRDHVQGPGEDRYLAPEISAVAELARTGALVGAAQSAA
                     GPLL"
     gene            1052..1675
                     /locus_tag="VGP27_20125"
     CDS             1052..1675
                     /locus_tag="VGP27_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEV7853641.1"
                     /translation="MTAASRERAALVDTMRAAGPDAPTLCGDWTTRDLAAHLILRERR
                     PDAAAGILIPKLAGYTARKQQQLAEGNQWPALLDMIASGPPLYSPLKLLDPLVNTTEM
                     FIHHEDVRRAAPGWQPRELDDDLATALRRQVRLAARMSLSRVPATVTLQDSDGTTLAS
                     VGKGPHCAVTGAAPELLLFLSGRDAAQIEFTGADDTVEQVRTARRRL"
     gene            1751..2641
                     /locus_tag="VGP27_20130"
     CDS             1751..2641
                     /locus_tag="VGP27_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HEV7853642.1"
                     /translation="MTLNTIALELVPPNVERGPEYAVEEARKVARISADSGLAGRIKH
                     VMIPGMIAEDDDRPVEMKPKLDVLDYWSLIQPELPGLRGLCTQVTAFMDEAALGERLT
                     TLGGAGFEGIVFVGVPRTLNDGEGSGVAPTDALSIYDRVVDNRGVIVIPTREGEHSRF
                     DFKCNQGANFGMTQLLYSDAIVGFLTDFAKNTDHRPEILLSFGFVPKVETRVGLIDWL
                     IQDPGNAAVADEQEFVRSLASRDPADKRQMMVDLYKRVIDGVADLGFPLSIHFEATYG
                     VSGAAFETFAEMLAYWSPEG"
     gene            complement(2638..3285)
                     /locus_tag="VGP27_20135"
     CDS             complement(2638..3285)
                     /locus_tag="VGP27_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEV7853643.1"
                     /translation="MLGELVPLALVVALSPVSIIPAVVLVLHTDHPRPTGLAFMTGWL
                     VGLLALTALLVQVPHVIDGLGASPPWMPLVRIGIGVLLIALAVGRWMTRKRTTRPPAL
                     LNSLSRITPAGAAAVGFGLVVANPKVLVMNATAGLIIGTAAAGARVWVAVALYTALAG
                     STVIAPVLAYVVAGGRVDQQLERVRNWMQREHAAVTAVILLAVGVLLAYTGIRAL"
     gene            complement(3385..3798)
                     /locus_tag="VGP27_20140"
     CDS             complement(3385..3798)
                     /locus_tag="VGP27_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7853644.1"
                     /translation="MYGNQAVDFDGAQMRAQCRQLATVVTVTGDLNDTNMDYVSAYAR
                     RFVLNEKPFVLDLTGVNSFTPECISLLYAIDENCHNAGVEWSVVASQPVHRTVRLFGE
                     GETFPTVGSVAEALHEFAISIGERRRLLPLLTKTA"
BASE COUNT          700 a         1326 c         1298 g          627 t
ORIGIN      
        1 gaagcgatgg ccgagcacgc gctcgtgccg gtgaccttcg ccgaaaagga aggcctggcg
       61 ctgatcaacg gcaccgacgg aatgctcggt caactggtgc tcgcgctcac cgaccttgac
      121 agcctgttcc ggctcgccga tatcgcggcg gcgatgagcg tcgagggcca gctcggcacc
      181 gaccgggtct tcgccgacga tctccaacgg ctgcgaccac accctgggca ggccctttcc
      241 gcggcgaaca tcacccggat gctggcgggg tcggcgatcg tggccagtca tcgcggcgac
      301 gactgcacgc gggtacagga cgcctactcg ctgcgatgct caccgcaggt cgccggcgcc
      361 gcgcgtgata cggccgagca tgcgcgcgcg gtggccgagc gtgaactggc aagcgtcatc
      421 gacaatcccg tgatcacgga agacgggcgc gtcgagtcga acggcaactt ccacggcgcc
      481 ccggtggcct acgtgctgga cttcctgtcg atcgtggtgg ccgatctggc cagcatcagc
      541 gaacgccgga ccgaccggtt cctcgacgtc gcccgcaacc acggcctcaa cccgttcctt
      601 gccgacgatc ccggcgtgga cagcggccac atgatcaccc agtacaccca ggccgcgatc
      661 gtgtccgagc tcaaacggct ggccgcgccc gcgagtgtcg actccatccc gtcgtcggcg
      721 atgcaggaag accacgtgtc gatgggctgg gcagccgccc gcaagctgcg ccggggcgtc
      781 gacggtctga cacaggtgct ggcgatcgag gtgctcaacg cggcccgcgg catcgagatg
      841 cgcctgccgt tgacgcctgc gccgggcacc gcggcggtca ttgccgcggt cagggaccat
      901 gttcagggac cgggtgagga tcgctatctg gcacccgaga tcagcgccgt cgccgagctt
      961 gcccgaaccg gcgccttggt gggagccgcg cagagcgccg ccggcccgct gttatgagtc
     1021 ggcagacgag ggcagcgggt atacggcgac catgacagca gcctcgcgtg aacgcgccgc
     1081 cctcgtcgac acgatgcgcg ccgccggacc cgacgcgccg acgttgtgcg gtgactggac
     1141 cacccgcgac ctcgccgcgc atctgatcct gcgcgaacgt cgtcccgacg ccgccgcggg
     1201 tatcctcatc cccaagctgg ccggctacac cgcccgcaag cagcagcagc tcgccgaggg
     1261 caaccaatgg cctgccctgc tggacatgat tgcgtcgggc ccgccgctgt actcgccgct
     1321 gaaactgctc gatcctctgg tcaacaccac ggagatgttc atccaccacg aagacgtccg
     1381 ccgcgcagcg cccggctggc agccacgtga gctggacgac gacctcgcga ccgcgctgcg
     1441 ccggcaggtt cggctggcgg cccggatgtc actcagccgc gtcccggcga cggtgacgtt
     1501 gcaggattct gacggcacca cgctggcgtc cgtcggcaaa ggcccgcact gcgcggtcac
     1561 cggggcggcg cccgaattgc tgctcttcct gtccggccgt gacgccgcac agatcgagtt
     1621 caccggcgcc gacgacaccg ttgagcaggt ccggacggcc cgccgccggc tgtagacggc
     1681 gatcggcgat ttcgaactac acagctgcgc tctgtatcgt gggtcggtcg tcttgatcag
     1741 gagtaacgct gtgaccctca acactattgc tctcgagctg gtgcccccga atgtggagcg
     1801 cgggccggag tatgcggtgg aagaggcgcg caaggtggca cgaatctccg cggactcagg
     1861 gctggccggc cgcatcaagc acgtgatgat tcccgggatg atcgccgagg acgacgaccg
     1921 gcccgtcgaa atgaaaccga agctcgacgt cctcgactac tggtcgctca tccagccgga
     1981 gctgcccggg ctgcgcgggc tgtgcaccca ggtgaccgcc ttcatggacg aggccgccct
     2041 tggcgagcga ctgaccaccc tcggaggcgc cgggttcgag ggcatcgtgt tcgtcggcgt
     2101 cccccgcacc ctgaacgacg gcgaaggatc cggtgttgcc ccaacggatg cgctgtcgat
     2161 ctatgaccgg gtggttgaca accgcggcgt gatcgtcatc ccgacccgcg aaggcgagca
     2221 cagtcgattc gacttcaagt gcaaccaggg cgccaacttc ggcatgacgc agctgctgta
     2281 ctccgacgcg atcgtcggtt tcctcaccga tttcgccaag aacaccgacc atcggcctga
     2341 aatcctgctg tcattcggct tcgtaccaaa ggtcgaaacc agggtcggcc tgatcgactg
     2401 gctcatccag gatcccggca acgctgcggt cgccgacgag caggagttcg tccggtcact
     2461 ggcgagccgc gatcccgccg acaagcgcca gatgatggtc gatctctaca agcgggtgat
     2521 cgacggtgtc gccgatctcg gcttcccgct gagcatccac ttcgaggcga cgtacggcgt
     2581 ctccggcgcg gctttcgaga cgttcgcgga gatgctcgcc tactggtctc cggagggcta
     2641 gagcgcgcga atcccggtgt aggccaacaa cacaccgacc gcgagcagaa tcacggccgt
     2701 cacggcggcg tgctcgcgct gcatccagtt gcgcacccgt tcgagctgtt gatcgacacg
     2761 tccgccggcc accacgtagg cgagtaccgg ggcgatgacc gtcgagccgg ccagtgcggt
     2821 gtagagggcg acggcgaccc acacgcgcgc gcccgctgcg gccgtgccga tgatcaagcc
     2881 cgcggtggca ttcatgacga gcaccttggg gttggccacc accagcccga agccgaccgc
     2941 cgccgcgccg gcgggcgtga ttctgctcag gctattgagc aaggcgggcg ggcgggtcgt
     3001 gcgcttgcgc gtcatccacc gcccgactgc gagggcgatc agcaggacac cgatcccgat
     3061 tcgcaccaag ggcatccacg gtggagacgc cccgagcccg tcgatgacat gcggcacctg
     3121 aaccaagagg gccgtcaacg cgagaaggcc cacaagccag cccgtcatga aggccaggcc
     3181 tgtgggcctt ggatggtcgg tgtgcagaac cagcacgacc gccggaatta tcgaaaccgg
     3241 tgacagtgcg acaacaagtg cgagcggtac cagctcgccg agcaagaagc cgggcggact
     3301 agcgcgcggg cgtgggtgtg ggggtgtgcc tgccgagcag gtaggtcaac cagcggagat
     3361 cgatgagcac gtatcgccct ttctttaggc ggtcttggtg agtagcggaa gcaggcggcg
     3421 acgttcaccg atgctgatcg cgaattcgtg cagcgcctcg gcgacggagc ccacggtggg
     3481 gaacgtctcg ccctccccga aaagccgcac tgtccgatgg accggttggc tggcgaccac
     3541 cgaccactcg acgccggcgt tgtggcagtt ctcgtcaatg gcgtagagca gtgaaatgca
     3601 ttccggcgta aacgaattca cgcccgtcag atccaacaca aagggctttt cgttgaggac
     3661 gaagcgccgt gcgtaggcac tgacgtaatc catgttggtg tcgttgagat cgccggtcac
     3721 cgtcaccacg gttgccagct gacgacactg cgcacgcatc tgcgcgccgt caaagtcgac
     3781 tgcttggttt ccgtacatga ggtcaaagct aagccgctga tctaaggcat tggggagcag
     3841 ccgctaatac tttgctaaga accgaaatcg gcgaactcgg ctcaacgatt gcccaattgt
     3901 gccccggagt agttctccca cgggctgtag tccgcgagca gcgcttcctg c
//
LOCUS       DASYLX010000861         3245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_271482,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000861 DASYLX010000000
VERSION     DASYLX010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271482"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGP27_20145"
     CDS             <1..262
                     /locus_tag="VGP27_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7853645.1"
                     /translation="RAFVFADASAAGEVDHVGKLMDSMFARAMSEGEPTEDQYHIARV
                     ISDVWLSNLLAWLTRRASATDVSKRLDLAVRLLIGDGEHPKI"
     gene            266..1009
                     /gene="otsB"
                     /locus_tag="VGP27_20150"
     CDS             266..1009
                     /gene="otsB"
                     /locus_tag="VGP27_20150"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEV7853646.1"
                     /translation="MLPVELQRALDALAAVPRLLVACDFDGTLAPIVNDPADARPLPA
                     AAESLAELARLPSTHVALVSGRALSVLKELARMPPEVNFVGSHGAEFDAGFAHQVDES
                     LLARITNALKDIATDRPGITVESKPASVALHVRNATQADGDAALREARAAATSWDAEL
                     TEGKAVLEFAVISTDKGEAVDILRERYDAAAVAFMGDDVTDEKAFRRLRDSDVGIKVG
                     EGETLAEYRVGSPDDVADVLEYLLRARRA"
     gene            complement(1011..2111)
                     /locus_tag="VGP27_20155"
     CDS             complement(1011..2111)
                     /locus_tag="VGP27_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7853647.1"
                     /translation="MNRGPTPRRRATLASLAAELKVSRTTISNAYNRPDQLSADLRER
                     VLATAKRLGYPGPDPVARSLRTRRAGAVGLVMTEPLNYSFSDPAALAFVAGLAESCED
                     AGQGMLLVAVGPNRSVSDGSAAVLAAGVDGFVVYSASDDDPYLQVVLQRHLPVVVVDQ
                     PKDVPAASRICIDDRASMAELAKYVVELGHREIGLLTMRLGRERPPGGGKPSVANPDR
                     LRTPNFHVQRERIHGVYDAMMDAGLDPASLTVVESYEHQSTSGGVAAEVALEANSRIT
                     ALICTADVLALSAMDYLRARGIYVPGQMTVAGFDGVPEALRRGLTTVKQPSLEKGVRA
                     GRLLHNPPRSGLPAIDVLDTELVRGRTSGPPG"
     gene            2199..3110
                     /locus_tag="VGP27_20160"
     CDS             2199..3110
                     /locus_tag="VGP27_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEV7853648.1"
                     /translation="MSVRATAIGLAVTLAAAVFAAAGCGQSASGHDHGGVDVVASTDV
                     WGSVAAAVAGPRISVKSIETGANADPHSFEATPADVAEITDASLLVYNGGGYDHWVDD
                     VLAGKPEANAVDAYSLRGTQGQANEHVFYDVGVAKAVADEIAKRLARIDSAHAGEYQA
                     NATAFGRRADDIAAAERAIGQAHPSESVVATEPVAFYLLRNAGIADRTPPGFASAVEE
                     GDDPSPADVAAMLDLINSHQVSVLLFNPQTETAATTQIEDAARRASLPVVTVTETLPR
                     GVDYLTWQRNTVDELASQFDKAPQTNR"
     gene            3118..>3245
                     /locus_tag="VGP27_20165"
     CDS             3118..>3245
                     /locus_tag="VGP27_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7853649.1"
                     /translation="MEAADVVDLKGGRLAFGDRVLWDELDLCVRAGEFIAVLGPNGT"
BASE COUNT          545 a         1084 c         1093 g          523 t
ORIGIN      
        1 ccgggccttc gtgttcgccg acgcatcggc tgccggcgag gtcgatcacg tcggcaagct
       61 gatggactcg atgttcgcgc gcgccatgag cgagggtgag ccgaccgagg accagtacca
      121 catcgccagg gtgatctcgg atgtctggct gtccaatctg ctggcctggc tgacgcgccg
      181 ggcgtcggcg accgatgtca gcaagcgact cgaccttgcc gttcgtctgc tcatcggcga
      241 cggcgagcac cctaagattt gacgcgtgct accagtcgaa ttgcagcgcg cgctcgatgc
      301 ccttgccgca gttcctcggc tgctggtcgc ctgtgacttc gacgggaccc tcgccccgat
      361 cgtcaacgat ccggccgacg cccgaccatt acccgccgcc gcggaatcgt tggccgagct
      421 cgcgaggttg ccatcgacac acgtcgcgct ggtgtccggc cgcgcgctca gcgtgttgaa
      481 ggagctcgcc cgcatgccgc cggaggttaa tttcgtgggc agccacggcg ccgagttcga
      541 cgccggattc gcacaccagg tcgatgaatc ccttttggct cgaatcacca acgcactcaa
      601 ggacattgcc accgaccgcc cagggatcac ggttgagtcc aaaccggcca gcgtggcgct
      661 gcatgtgcga aatgccaccc aggcggacgg cgacgcggca ctgcgcgaag cgcgcgcggc
      721 cgcgacatcc tgggatgctg aactcaccga gggcaaggcc gtgctcgaat tcgcagtcat
      781 ctccacggac aagggtgagg ccgtggacat cctgcgcgaa cggtatgacg cggcagcggt
      841 cgcgttcatg ggcgacgacg tcaccgacga gaaggcgttc cgccgactgc gcgattccga
      901 tgtgggtatc aaggtcggcg aaggcgagac gctggccgaa taccgggtcg gctcgcccga
      961 cgacgtcgca gacgtgctcg agtacctgct tcgcgcgcga cgcgcctgac tcagcccggc
     1021 gggcccgagg tgcgaccgcg gaccagctcc gtgtcgagca cgtcgatcgc cggtagcccg
     1081 gaccgcggcg ggttgtgcag cagtcgtccc gcccgcacgc ccttttcgag gctcggctgt
     1141 ttcaccgtcg tcaggccgcg gcgcagcgct tcgggcacgc cgtcgaatcc ggccaccgtc
     1201 atctggcccg gcacatagat tccgcgcgcg cgcagataat ccatcgccga gagggccagc
     1261 acgtccgcgg tacagatcaa cgccgtgata cgcgagttgg cctcgagcgc cacctctgcg
     1321 gccaccccgc ccgaagtcga ctgatgctca tagctttcca ctacggtcag cgacgccgga
     1381 tcgagtcccg cgtccatcat ggcgtcgtaa acaccgtgga tccgttcccg ttgcacgtga
     1441 aagttcggtg tccggagccg atccgggttt gcgaccgagg gcttgccgcc gcccggcggg
     1501 cgttccctgc ccagccgcat ggtcagcagc ccgatctcgc ggtggcccag ctccacaacg
     1561 tatttggcca gttcggccat gctcgcccga tcgtcgatac agatccggga agctgcgggc
     1621 acatccttgg gctggtcgac gacgacgacc ggcagatgcc gctgcagcac cacctgtaaa
     1681 tacgggtcgt catcggatgc ggagtacacc acgaagccgt cgacacccgc agcgagcacg
     1741 gcggccgatc cgtcgctcac cgaccggttc ggtccgaccg cgaccagcag catcccctgg
     1801 cccgcgtcct cacacgactc cgccagcccg gcgacgaagg cgagcgccgc gggatcgctg
     1861 aacgaatagt tcagcggttc ggtcatcacc aggccgacgg cgccggccct gcgggtgcgc
     1921 agcgagcggg ccaccgggtc gggcccggga tacccgagcc gcttggccgt cgccaacacc
     1981 cgttcgcgta gatctgctga cagttggtcg ggccggttgt aggcgttgga gatcgtggtc
     2041 cgcgaaacct tgagctcggc ggccagggaa gccagggtcg cccgacgccg cggcgtggga
     2101 cccctgttca tgctgcgacg ttagctgacg ttcctgttcg cagagcggca ccagttgcgt
     2161 tagagttatt ggaaacggtt ttcatttgta ttaggaggat gtcggtgcgc gctacggcga
     2221 tcggacttgc agtgacgctg gcagcggccg tgttcgccgc ggcgggttgt ggccagagcg
     2281 cgtcaggtca tgatcacggc ggcgtcgacg tcgtcgcaag caccgacgtg tggggcagtg
     2341 tggccgccgc ggtggcgggc ccgcgtatat ctgtgaaatc gatcgagacc ggcgcgaacg
     2401 ccgacccgca ctcgttcgaa gcgactcccg ccgatgtcgc cgagatcacc gacgcgtcgc
     2461 tgctggtcta taacggcggc gggtacgacc actgggtcga cgacgtgctg gcgggtaagc
     2521 ccgaggccaa tgccgtcgac gcctactcgc tgcgcggcac acagggacag gcgaatgagc
     2581 acgtcttcta cgacgtcggc gtcgccaagg ccgtcgccga cgagatcgcg aagcggctgg
     2641 cgaggatcga ctccgcgcat gcaggcgaat accaggcgaa tgcaaccgca ttcggacggc
     2701 gggccgacga catcgcggcc gcagagcggg ccatcggaca ggcacacccg tcggagtcgg
     2761 tggtggcgac cgaaccggtg gcgttctacc tgctgcgcaa tgccggcatc gccgatcgca
     2821 cgccgcccgg cttcgccagt gcggtggagg agggcgacga tccgtcaccc gccgacgtcg
     2881 ccgcgatgct cgacctcatc aacagccacc aggtatcggt gctgctgttc aacccgcaga
     2941 ccgagactgc ggccaccaca cagatcgagg acgcggcgcg gcgggcttcg ctgccggtgg
     3001 tcacggtgac cgaaaccctt ccccgaggcg tcgactatct gacgtggcag cgcaataccg
     3061 tcgacgagtt ggccagccaa ttcgacaaag cgccacagac gaatcgatag tgacctggtg
     3121 gaggctgcag acgtcgtcga cctgaagggt ggccggctcg ccttcggcga ccgggtgttg
     3181 tgggacgaac tggacctgtg tgtgcgggcg ggcgagttca tcgcggtgct cgggccgaac
     3241 gggac
//
LOCUS       DASYLX010000862         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2793229,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000862 DASYLX010000000
VERSION     DASYLX010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793229"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGP27_20170"
     CDS             <1..295
                     /locus_tag="VGP27_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853650.1"
                     /translation="PKPFDSIVPAELPGSARVYTHASGVAEMVTGGLLLAPRTRKLGA
                     LAAVALFVSVLPANVNMVRLWKDKPLPMRIGAIARLPLQIPMITQALKIYRES"
     gene            complement(273..887)
                     /locus_tag="VGP27_20175"
     CDS             complement(273..887)
                     /locus_tag="VGP27_20175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853651.1"
                     /translation="MASDDGGRPNQRSRTRKDLLAAASRLLKQGRRPTLEEVAEEALV
                     SRATAYRYFPSVDALLVEAPLDGATPNPDELFRDASSGDAVERLELVDAALDDMIGAN
                     EQALRLMLAHSLQRTANGEGPNGFPARQNRRSALIGAALESAGEDIEPALAQRLAHAL
                     ALIIGTESMVVLKDVLALDASEATEVKRWAIRALVDAAKTPGRS"
     gene            complement(892..1314)
                     /locus_tag="VGP27_20180"
     CDS             complement(892..1314)
                     /locus_tag="VGP27_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853652.1"
                     /translation="MALTPAEMDQKIDEHFDFEARDDIEGVLATLAPDAEHDIVGHPT
                     GPTVGRDAARAFYEGLFNDLSESSIETKRRLYGDGFIVDESLWCGRAPGTPFGLQGRN
                     RPLEFRLLHIVEFTDAGAIARENVWIDFAAIVAQLPQV"
     gene            complement(1364..>2900)
                     /locus_tag="VGP27_20185"
     CDS             complement(1364..>2900)
                     /locus_tag="VGP27_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7853653.1"
                     /translation="LPDLVAEVRRVLGVDAEARAARPPSAGWTGTEHLDAFADVVADY
                     SAARTGARVSDLLAYLDAAMVVENGLAPAVATVAHDRVQILTVHAAKGLEWQIVAVPH
                     LSGRVFPSTASMRTWLTDASDLPPLLRGDRATMGDHGVPVLDTSDVNDRKVLSDKIAE
                     HKRRLDQRRVDEERRLLYVALTRAEDTLLLSGHHWGASEGKPRGPSEFLLELKEIIDA
                     SATAREPCGVVEHWAPAPTEGDLNPLRDKAIEAMWPADPAGLRRGDVDRGAVLVRHAM
                     AGRLDGPDDDLDGWVADVDALLAERVRVPPQRPVTLPAALSVSSLVDLGRDPDAAVRR
                     LRRRLPVRPDPHALLGTAFHDWVQRFYGAERLFDLDDLPGAVDGQVARENAESLAELQ
                     AAFMASPWAARTPVDVEVPFDMVIGGTVVRGRIDAVFVDPDGATVVDWKTGDPPDTPE
                     AKRHAAIQLAVYRLAWAALRDVPVDSVRAAFHYVRSGQIVVPDALPDLDDLAVLLEVD
                     KIA"
BASE COUNT          459 a         1030 c          943 g          468 t
ORIGIN      
        1 ccccaaaccc ttcgactcga tcgtccccgc cgaactgccc ggctccgcgc gcgtctacac
       61 ccacgcttca ggggtcgcag agatggtcac cggcgggctg ctgctggcac cgcgcacccg
      121 caagctgggc gcactggccg ccgtggcgct gttcgtgtcg gtgttgccgg ccaacgtgaa
      181 catggtccgg ttgtggaagg acaagccgct gccgatgcgc atcggcgcga tcgcccggct
      241 gccgctgcag attccgatga tcacccaggc gctcaagatc taccgggagt cttagcggcg
      301 tcgaccagcg cacggatggc ccaccgtttc acctctgtgg cctctgacgc gtcgagggca
      361 aggacgtcct tgaggaccac catcgactcg gttccgatga tgagcgccaa cgcgtgcgcc
      421 agccgctgtg ccagtgcggg ctcgatgtcc tcaccggccg actccagggc tgcgccgatc
      481 aatgccgacc gccggttctg ccgcgccgga aacccgttcg gcccttcgcc attcgcagtg
      541 cgctgcaacg agtgcgccag catcaaccgc agcgcctgct cattggcgcc gatcatgtca
      601 tcgagggccg cgtcgacgag ttcgagacgc tcgaccgcat caccggaaga ggcatcgcga
      661 aacagctcgt caggattcgg tgtcgccccg tcgagtggcg cttcgacgag cagagcgtcg
      721 acgctcggga agtatcgata agccgtcgcc ctggagacca gcgcctcttc ggcgacctcc
      781 tccagcgtcg gccgacggcc ttgcttcagc agccgcgagg ccgcggcgag caggtccttt
      841 cgggtacggc tgcgctggtt gggcctaccc ccgtcgtccg aagccattgc gtcacacctg
      901 cggcagctgt gcgacgatcg ctgcgaaatc gatccagaca ttctcccgcg caatggctcc
      961 ggcgtcggtg aactcgacga tgtgcaacag ccggaactcg agtggccggt tcctgccctg
     1021 caatccgaac ggtgtgccgg gtgccctgcc gcaccagagc gattcgtcga cgatgaagcc
     1081 gtcgccgtag agcctgcgct tggtctcgat actgctttcc gacagatcgt tgaacaagcc
     1141 ctcatagaaa gcccgcgcgg catcgcggcc cacggtcggg cctgtgggat ggcccacgat
     1201 gtcatgttcg gcgtccggcg caagagtggc cagcacgcct tcgatgtcgt ctcgggcctc
     1261 gaaatcgaaa tgctcgtcga tcttctggtc catctcagca ggggtcaacg ccatgcccag
     1321 caccttaata agactattgt ctcatgtcaa tactgatatc tctctatgcg attttgtcga
     1381 cttccaacag caccgccagg tcgtcgaggt ccggcagcgc gtcgggcacg acgatctggc
     1441 cggaccgcac gtagtggaac gccgcgcgga cggagtcgac gggcacgtcg cgcagcgccg
     1501 cccacgccaa ccggtagacg gcgagctgga ttgccgcgtg gcgcttggcc tctggcgtat
     1561 cgggcgggtc gccggtcttc cagtcgacga cggtcgcccc gtcggggtcg acgaacaccg
     1621 cgtcgatgcg gccccgcacc accgtgccgc cgatgaccat gtcgaagggc acctcgacat
     1681 cgacgggggt ccgggcggcc cacggcgaag ccatgaacgc ggcctgtagt tcggccaggc
     1741 tttcggcgtt ctcacgggcc acctgcccgt cgaccgctcc cggcaggtcg tcgaggtcga
     1801 agagccgctc ggccccatag aaccgctgca cccagtcgtg aaacgcagtg cccagcaggg
     1861 catgtggatc tgggcgcacc ggcagcctgc ggcgcaagcg gcgcaccgcc gcgtcgggat
     1921 cgcggccgag gtccaccagg ctgctcaccg acagcgcggc gggcagcgtg acgggccgtt
     1981 gcggtggcac ccggacccgc tcggcgagca gagcatcgac atcggccacc cagccgtcga
     2041 ggtcgtcgtc cggcccgtca agccttcccg ccatcgcatg ccggacaagg acggcccccc
     2101 ggtccacatc gccgcggcgc agccccgccg gatcggcggg ccacatcgcc tcgattgctt
     2161 tgtcgcgcaa cgggttcaga tcaccttcgg tcggcgcggg cgcccagtgc tcgaccaccc
     2221 cgcacggttc tcttgcggtc gccgaagcgt cgatgatctc cttgagttcg aggaggaact
     2281 ccgagggccc gcgcggcttc ccctcggatg cgccccaatg atggcccgac agcagcaggg
     2341 tgtcctcggc gcgggtcagc gccacgtaca gcagcctgcg ctcttcgtcg acgcgacgct
     2401 gatcgagccg ccgtttgtgc tcggcgatct tgtcggacaa cacttttcga tcgttcacat
     2461 ccgacgtgtc gagcaccggg acgccgtggt cgcccatggt ggcgcggtcg ccgcgcagca
     2521 ggggcggcag gtcggacgcg tcggtgagcc aggtccgcat cgacgccgtc gacgggaaca
     2581 cgcgtccgct caggtgcggc acggccacga tctgccattc gaggcccttg gccgcgtgca
     2641 ccgtcaggat ctgaacgcgg tcgtgcgcca cggtggccac cgcaggagcc agcccgtttt
     2701 ccaccaccat tgcggcgtcg aggtaggcga gcagatcgga caccctcgcg cccgtgcgcg
     2761 ccgccgagta gtcggccacc acgtcggcga aagcgtcgag atgttcggtt ccggtccacc
     2821 ccgccgacgg cggccgggcc gcacgggctt cggcgtcgac gccgagcacc cgacgcacct
     2881 cggcaaccag atccggcagc
//
LOCUS       DASYLX010000863         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2026629,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000863 DASYLX010000000
VERSION     DASYLX010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026629"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VGP27_20190"
     CDS             <1..726
                     /locus_tag="VGP27_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7853654.1"
                     /translation="YRHIDTAEMYGNEKGVGQGVRDAGLDRADVFITSKLNNGFHKPD
                     DARRAFDNTLKALGSDYVDLFLIHWPLPTLYGGDFVSTWNVLEEFAKEGRARSIGVSN
                     FQPAHLDRLAAESDTVPAVNQIEAHPYFTNDEVRAYGRKHGIATEAWSPIAQGKVLGD
                     PVVERIADAIDRSPAQVVLRWHIQRGDIVFPKSVSPARMKSNFQLFDFELRNSAMDAI
                     SALDHGESGRTGPNPDKFDYIPK"
     gene            767..978
                     /locus_tag="VGP27_20195"
                     /pseudo
     CDS             767..978
                     /locus_tag="VGP27_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1215..1346)
                     /locus_tag="VGP27_20200"
     CDS             complement(1215..1346)
                     /locus_tag="VGP27_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853655.1"
                     /translation="MHTKLTYIAALFVATAAAVAVHAAPAAAAATENSCAAGASATV"
     gene            complement(1442..>1831)
                     /locus_tag="VGP27_20205"
     CDS             complement(1442..>1831)
                     /locus_tag="VGP27_20205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV7853656.1"
                     /translation="GLGFAIPVDEAKRIADELIHTGTASHGFLGARLADDGNANGARI
                     LEVSSGGPAAAADLPAGALVTKVDDQLIDNADALGSVVLTKAPGTTVTVDYLDPSGTV
                     RTAHVLLGTDQGQQSSVPLSGSGKGTS"
BASE COUNT          354 a          571 c          588 g          318 t
ORIGIN      
        1 taccggcaca ttgacaccgc agagatgtac ggaaacgaga agggggtcgg ccagggcgtc
       61 cgcgacgccg gattggaccg cgccgatgtc ttcatcacga gcaagctcaa caatggcttc
      121 cacaagccag acgatgcgcg ccgcgcgttc gacaacacgc tgaaagcgct cggatccgac
      181 tacgtggatc tgtttctcat ccactggccg ctgccgacgc tctatggcgg tgacttcgtc
      241 tcgacctgga atgtcctcga ggagttcgcc aaggagggac gcgcccgcag catcggagtg
      301 tcgaacttcc agcccgcaca tctggatcga ctggcagcgg agtccgacac cgtacccgcg
      361 gtcaaccaga tcgaggccca tccgtatttc accaacgacg aggtccgcgc gtacggacga
      421 aagcatggca tcgcgaccga ggcgtggtcg ccgatcgctc agggaaaggt gctcggtgac
      481 ccggtcgttg agcgaatcgc agacgccata gaccggtcac ccgcacaggt cgtgttgcgg
      541 tggcacatac aacggggcga catcgtgttc ccgaaatctg tatcgccggc tcggatgaag
      601 tccaattttc aactgttcga cttcgaactg cgcaactccg caatggacgc gatctcggcc
      661 ctcgatcacg gcgaatccgg tagaaccggc cccaaccccg acaagttcga ctacataccc
      721 aagtaacgct gatcgtcggc cgctcgagag ccacggcaac gcggatccct ctgccggttg
      781 gggcgcggcc cgcagcgcaa aggtgttggc gcagagccgg gagctggtcg cgggcacgcg
      841 ggcgacccag cggatgaacc acgagaacgg cggctttgac tgtccgggtt gcgcatggcc
      901 gacgacacca agggtctgca cctgtacatc tgcgagaacg ggatcaagca catcacttgg
      961 gtgatgaccc gcaagaggca agctcaaccg cagccacctg gtgcacggcc gcaaagcgct
     1021 gatcctgtcc tgcctggggc gcaccgagaa ggacattcaa cagaccggag cagggtgtca
     1081 ccgtcgagga ctcgatgagc atggtgcact tttcgtttgg gatgaagaag cccgagcgcg
     1141 agtccgtatc cgccgtaggg atggaagttt actggcggag tggcgtcatt gatctgcgca
     1201 ttgccgggcg actgtcacac ggttgccgat gcaccggcgg cgcaggagtt ttcggtggct
     1261 gcggcggcgg cgggtgcggc gtgaaccgcc acggcggccg cggtggctac gaacagcgct
     1321 gcgatgtatg tcagtttcgt atgcatgatg tgctcctgtc acgggggcct aatctggtct
     1381 gactccaggg tgatcctcga tcgaccgcat gtcgtccccg cggcccccat cggcgtaccc
     1441 gctaactggt accttttccc gagccggaca acggcactga cgactgctgt ccctgatcgg
     1501 tgccgagcaa gacatgcgcg gtgcggacag tccccgatgg gtcgagatag tccacggtga
     1561 ccgtggtgcc gggcgccttc gtcagcacca cgctgcccaa tgcgtcagcg ttgtcgatca
     1621 gttgatcgtc gaccttcgta accaatgcgc ctgcgggcag gtcggccgcc gccgcggggc
     1681 cgccgctcga gacctcgagg atccgggcgc cgtttgcatt cccgtcgtca gcaagccgcg
     1741 caccgagaaa cccatgagag gcagtgccgg tgtggattag ttcatcggcg atgcgtttgg
     1801 cctcgtcgac gggaatcgcg aaaccaaggc c
//
LOCUS       DASYLX010000864         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_819961,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000864 DASYLX010000000
VERSION     DASYLX010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819961"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VGP27_20210"
     CDS             complement(<1..948)
                     /locus_tag="VGP27_20210"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV7853657.1"
                     /translation="MPSDPGSIDIKPRSRDVTDGLEKAAARGMLRAVGMEDDDFAKPQ
                     IGVGSSWNEITPCNLSLDRLAKAAKDGVFAAGGFPMEFGTISVSDGISMGHEGMHFSL
                     VSREVIADSVEVVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGSILPGK
                     AKLSDGTEMDVTIIDAFEAVGACARGLMPREDVDAIERAICPGEGACGGMYTANTMAS
                     AAEAMGMSLPGSAAPPATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAV
                     VMAFGGSTNAVLHLLAIAHEADVKLSLEDFTRIGQKVPHL"
     gene            1026..1316
                     /locus_tag="VGP27_20215"
     CDS             1026..1316
                     /locus_tag="VGP27_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV7853658.1"
                     /translation="MTAPHGYTSNKDNYAKRLRRIEGQVRGIAKMIDDDKYCIDVLTQ
                     ISAVNSALQSVALNLLDEHLGHCVTQAVAEGGDEADTKLAEASAAIARLVRS"
     gene            complement(1317..1616)
                     /locus_tag="VGP27_20220"
     CDS             complement(1317..1616)
                     /locus_tag="VGP27_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV7853659.1"
                     /translation="MLVAFSVSPTGGDDTGGVSAAVAEAVRVVRASGLPNETNAMFTN
                     VEGEWDEVMAVVKQAVDAVAAVAPRVSLVLKADIRAGHSGQLTAKVESIERELGG"
     gene            complement(1619..2008)
                     /locus_tag="VGP27_20225"
     CDS             complement(1619..2008)
                     /locus_tag="VGP27_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7853660.1"
                     /translation="MVAIPEGYESLLEQPNFGHLATVRPDGSPQVNPMWFDWDGQLLR
                     FTHTTKRQKFRNFAANPNVAMSVVDSENPYRYLEVRGVVEEIVPDPTGAFYMQLNDRY
                     SGPLTEPPPDKADRVIIVVRPTGFTKQ"
BASE COUNT          329 a          690 c          662 g          336 t
ORIGIN      
        1 caggtgcggc accttctggc cgatccgggt gaagtcctcg agcgacagct tgacgtcggc
       61 ttcgtgcgcg atcgccagca gatgcagcac cgcattggtg gagccgccga acgccatcac
      121 caccgcgatc gcgttctcga acgcctcctt ggtcaggatg tcgcgggcgg tgatgccgcg
      181 gcgcagcagc tcgacgacgg cctgcccgct gcgccgggcg aacccgtcgc gccgccggtc
      241 ggtggcgggt ggggccgcgc tgcccggcag ggacatgccc atcgcctcgg cggccgacgc
      301 cattgtgttg gcggtgtaca tgccgccgca ggcaccctcg ccggggcaga tcgcccgttc
      361 gatcgcgtcg acgtcctcac ggggcatgag cccgcgtgca catgcgccga ccgcctcgaa
      421 cgcgtcgatg atcgtcacgt ccatctcggt gccgtcggag agcttggcct tgccgggcag
      481 gatcgaaccg gcatagagga agacacttgc gaggtcgaga cgggcggcgg ccatcagcat
      541 cccgggcagc gacttgtcgc agccggccag cagcaccgac ccgtcgaggc gctcagcctg
      601 catgacgacc tcgacgctgt ccgcgatcac ctcacgcgag accagcgaga agtgcatgcc
      661 ttcgtggccc atcgaaatcc cgtcggacac cgagatggtg ccgaactcca tcgggaagcc
      721 gccggcggcg aagacgccgt ccttggcggc cttggccagc cggtccagcg aaaggttgca
      781 cggcgtgatt tcgttccacg acgacccgac accgatctgc ggcttggcga agtcgtcgtc
      841 ctccatgcct acggcccgaa gcattccgcg ggcggcggct ttttcgaggc cgtcggtcac
      901 gtcgcggctg cggggcttga tgtcgatgga accggggtct gagggcatga ctgccagtat
      961 gctcgctgcg tttcgcccgg ccaaaccgat atgcataccc cggtggggta ccgtacgctg
     1021 gtggtatgac tgccccacac ggctacacgt ccaacaagga caactacgcc aagcggttgc
     1081 ggcgcatcga gggtcaggtc cgcggcatcg cgaagatgat cgacgacgac aagtactgca
     1141 tcgacgttct cactcagatc agcgccgtga acagcgcgct gcagtcggtt gccctgaacc
     1201 tgctcgacga gcacctcggc cactgcgtga cgcaggcggt cgccgagggc ggtgacgagg
     1261 ccgacacgaa gctggccgag gcgtcggccg ccatcgcgcg actcgttcgg tcgtagctac
     1321 ccgcccagct cccgctcgat actctcgacc ttggccgtca gctgaccgct gtggccggcc
     1381 ctgatgtcgg ctttgagcac caggctgacg cgcggcgcca ccgcggccac ggcgtcgacc
     1441 gcctgcttga cgaccgccat cacctcgtcc cactcgccct cgacgttggt gaacatcgcg
     1501 ttggtctcgt tgggcagacc agaagcgcga accacccgaa ccgcttcggc gacggctgcc
     1561 gacaccccac cggtgtcgtc gccacccgtc gggctgacgc tgaaggcgac cagcattgct
     1621 actgcttcgt gaacccggtc ggccgcacga cgatgatgac gcggtcggcc ttgtcgggcg
     1681 gcggctcggt cagcggaccg ctgtagcggt cgttcaactg catgtagaac gcgcccgtcg
     1741 ggtccggaac gatctcttcg acgacgccgc ggacctcgag atagcggtac gggttctccg
     1801 aatcgacgac cgacatcgcc acgttcggat tggccgcgaa gttgcggaac ttctgccgct
     1861 tcgtggtgtg ggtgaaccgc agcagttgcc cgtcccagtc gaaccacatc gggttcacct
     1921 gcgggctacc gtcgggtctg acggtggcga gatgcccgaa gttgggctgc tccagcagtg
     1981 attcatagcc ctcaggaatt gcaaccatcg cgagcct
//
LOCUS       DASYLX010000865         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1038313,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000865 DASYLX010000000
VERSION     DASYLX010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038313"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGP27_20230"
     CDS             <1..397
                     /locus_tag="VGP27_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7853661.1"
                     /translation="GTLDGTRVLSEESVRLMRTDRLTEEQRRFPFLGMPYWIGRGFGL
                     NLSVVTDPAKSRQLFGPGGKGSFTWPGAYGTWWLADPSADLILIYLIQNQPDMSADAG
                     SAVAGNTSLAKLQGVQPKFVRRTYQALGI"
     gene            483..1694
                     /locus_tag="VGP27_20235"
     CDS             483..1694
                     /locus_tag="VGP27_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7853662.1"
                     /translation="MAQPTVVISGAGIAGPALARWLTKNGYRVIVVEVAHGIRPGGQT
                     VDLRGAGGEVVERMGLMPEMRRRALEQHGVAWVASDGSRRAEMPVTAFDGNGLVSKLE
                     ILRGDLVDVLYQATKDSVEYRFNTRISALERDDDGVDVTLADGTSLRADLVVGADGPH
                     SAVRGLAFGPEHQFVEPLGGYNAWFTAPDHVGLAGWYLMYLAPGGLNASMRPTHDPAT
                     AKAGLAFASEPIVYDRHDLDGQRQLLVERFAGAGWQCDALLEAAQQADDFYFDSFLQV
                     KMPTWTSGRVTLVGDAGYCASPLSGMGTSLALVGAYLLAGELGPAESSDAQRIQEALQ
                     RYGSKMRPYVEKCQDIPNRVDRYAPNSPSDIATNIAVMKWMQRWPFRPIATRIWFTAA
                     DSIDLPTYQTL"
     gene            complement(1724..>1940)
                     /locus_tag="VGP27_20240"
     CDS             complement(1724..>1940)
                     /locus_tag="VGP27_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV7853663.1"
                     /translation="DRAILRVAVWELLHAEDVPEPVAVDEAVELAKHLSTDESPGFVN
                     GVLGQVMLVTPQIRAASAAIRGGSDNP"
BASE COUNT          326 a          660 c          623 g          331 t
ORIGIN      
        1 cgggacgctc gacgggactc gggtgttgtc cgaagagtcc gtgcgattga tgcgcacgga
       61 ccggctgacc gaggagcaga ggcggtttcc ctttctgggg atgccctatt ggatcggtcg
      121 cgggttcggc ttgaacttgt cggtcgtcac cgatccggcg aagtcgcggc agttgttcgg
      181 ccccggcggc aagggcagct tcacgtggcc gggtgcatac ggcacatggt ggctggccga
      241 tccgtcggcg gatctcatcc tgatctactt gattcagaac cagccggaca tgtcggctga
      301 tgccgggtcg gcggtcgccg gtaacacgtc gctcgccaaa ttgcagggcg tgcaaccgaa
      361 attcgtgcgc cgcacgtacc aggcgctcgg tatctgattt gtagcgcgcc gctccacctt
      421 tgcttagcga cccacaccga cacgtgacct gtgacgcgcg acgcgacccc tactctggtg
      481 cagtggcaca accgacggtg gtgatcagcg gcgcaggcat cgccggaccc gcactggccc
      541 gatggctcac caagaacggc taccgcgtga tcgtcgtcga ggtcgcgcac ggcattcgtc
      601 ccggcggcca aaccgtcgac ctacgcggcg ccggtggcga ggtcgtcgag cgcatgggcc
      661 tcatgccgga gatgcggcgc cgcgccctcg aacagcacgg cgttgcctgg gtggcgtccg
      721 acggcagccg ccgcgccgag atgccggtca ccgccttcga cggcaacggg ctggtgtcca
      781 agctggagat ccttcgcggc gacctcgtcg acgtgctgta ccaggccacg aaggactccg
      841 tcgaataccg cttcaacaca cgtatttcgg cgctcgagcg agacgacgac ggcgtcgacg
      901 tcaccctcgc ggacggcact tcgctgcgcg ccgatctcgt cgtcggcgcc gacggtccgc
      961 attccgccgt gcgcggcttg gcgttcgggc ctgagcacca gttcgtcgag ccgctcggcg
     1021 gttacaacgc atggttcacc gcgcccgacc acgtcgggtt ggccggctgg tacctgatgt
     1081 acctggcgcc cggcggtctc aacgcctcca tgcggcccac ccatgacccc gcaacggcca
     1141 aggccggtct ggcttttgcc tcggagccga tcgtctacga ccgtcatgat ctcgacgggc
     1201 agcgtcagct cctcgtcgaa cggttcgccg gcgccgggtg gcagtgcgat gccctgctcg
     1261 aagccgcaca gcaggccgac gacttctact tcgactcttt ccttcaggtg aagatgccga
     1321 cctggacatc gggccgcgtc accctcgtcg gcgatgccgg gtactgcgcc tcaccgctca
     1381 gcggcatggg caccagcctg gcactggtcg gcgcctacct ccttgccggt gaactcgggc
     1441 cagcggaatc gtctgacgcg caacggattc aagaagcgtt acagcggtat ggaagcaaga
     1501 tgcgtcccta cgtcgagaag tgccaggaca tccccaaccg cgtcgaccgc tacgcaccca
     1561 actcgccctc ggatatcgcg acgaacatcg cggtgatgaa gtggatgcag cgctggccgt
     1621 tccggccgat tgcaaccagg atctggttca ccgccgcaga ttcgatcgac ctgccgacct
     1681 accagacgct gtgacaagag agtagaagac gcggccgaac tggtcaggga ttgtccgaac
     1741 cgccccgaat ggcagccgag gccgcccgaa tctgcggcgt caccaacatc acctgaccga
     1801 gcacgccgtt gacgaacccg ggcgattcgt cggtggacag atgcttggcc agttcgacgg
     1861 cctcgtcgac ggccaccggt tcgggcacat cctcggcgtg cagcagttcc cagaccgcga
     1921 cccgcaggat cgcccgatcg
//
LOCUS       DASYLX010000866         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2137201,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000866 DASYLX010000000
VERSION     DASYLX010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137201"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGP27_20245"
     CDS             complement(<1..228)
                     /locus_tag="VGP27_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEV7853664.1"
                     /translation="MSAGLFGLLDDVAAIARMAAASVDDIGAAAGRATAKAAGVVIDD
                     TAVTPQYVHGIAAERELPIIKRIAIGSLRNKL"
     gene            complement(266..1111)
                     /locus_tag="VGP27_20250"
     CDS             complement(266..1111)
                     /locus_tag="VGP27_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEV7853665.1"
                     /translation="MRLLVAVFAGLLCCGFYDDARASASLGTDTAGMLAFGGLNRTYQ
                     LHVPGGVDHPAGLVINLHGAGMTGSAQAAATNYNAVADQYGFAVAYPDGIDMSWADGR
                     GASVPDRQGVDDVGFLVTLIDRLSRDYGVPSGRVFVTGMSAGAFMASRLACDRADVVA
                     AIAPVSGTLGAGTRCTPSRPVSVLEIHGTSDRVVPYAGGGMVGRGGPSDIEPAETMVS
                     GWRNVDGCPGAPVEDVVGGMHRFTSRGCADGTEVVLVRIDGGQHVWPGGASHDSAQFF
                     AAHGR"
     gene            1268..>2578
                     /locus_tag="VGP27_20255"
     CDS             1268..>2578
                     /locus_tag="VGP27_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7853666.1"
                     /translation="MSKYLYAVGGFSFRRRWLVLGTWLAILVGVMTAGLGLGGEPSDD
                     FSIPGTESQRAVEVLAQKLPAFSGAQTQITFAARGGARLTDPAVSSGIDQSIANLRSI
                     PQVAVAAGPAQTRLVSPNGQVGLGTVQWKAQPGEVDDPALQAVEAAMRPAQAAGVQVE
                     YSGSVFPGYKVSVPELPEIIGIIVAFGILLVTFGSVVAAGLPIVTACIGVGIGALGIF
                     AVAALVDMPSAALSLALMLGLSCGIDYALFILNRYRNNLILLKPKEESAALAVGTAGG
                     AVVFAALTVIIALCGLSIVGIPFLTYMGVAAAASVLIALMIAVTLLPALLGFAGHRVA
                     KFIPTPFDRAKETAQVAAFTPHRTMGARWGRFVVSMRKPLLLAGTVALLVLGLPAFSM
                     HLGLPSGASQPESNTSRRAYDLTSQHLGPGFNGPLLVVADLTRAN"
BASE COUNT          415 a          901 c          828 g          434 t
ORIGIN      
        1 cagtttgttg cgcagggaac cgatggcgat ccgtttgatg atcggcagct cacgctcggc
       61 ggcgatgccg tggacgtatt gcggcgtgac cgccgtgtcg tcgatcacga cacccgcggc
      121 cttggccgtc gcacgtcctg cggcggctcc gatgtcatcg accgaggcgg ccgccatcct
      181 cgcgatcgcg gcaacgtcgt caagaagccc gaaaagaccg gcgctcatcg cgtctccgtc
      241 attgcgttgc aggttaccgt gcgcactacc tgccgtgggc agcgaaaaac tgagcgctgt
      301 cgtgcgacgc accacccggc caaacgtgct gaccaccgtc gatccggaca agaacgactt
      361 cggtgccgtc agcgcaaccc cgcgacgtga agcgatgcat cccgccgacg acatcctcga
      421 cgggtgcacc ggggcagcca tccacgttcc gccacccgct caccatggtt tctgcaggct
      481 cgatgtcgct cggtccccca cgaccgacca ttccgccacc ggcatacggc actacgcgat
      541 ccgaggtgcc gtggatttca agcaccgaaa ccggacgaga gggagtgcat cgcgttcccg
      601 cgcccaacgt gccggagaca ggagcgatcg cggcgacgac atcggcgcga tcacacgcca
      661 gccgactggc catgaaggcg cccgccgaca tcccggtcac gaagactcgg cctgacggca
      721 cgccgtagtc gcgcgacagc cggtcgatga gcgtgacgag aaagccgaca tcgtccacgc
      781 cctgacggtc cggcaccgac gcgcctctcc cgtcggccca gctcatgtcg atgccgtccg
      841 ggtaggccac cgcgaatccg tattggtcgg caacggcgtt gtagttcgtc gccgcagctt
      901 gcgcgctgcc cgtcatcccg gcgccgtgca ggttgatcac caggccggcc ggatggtcga
      961 cgccgccagg cacgtgcagc tgataggtgc ggttcagccc gccgaacgcc agcatgcccg
     1021 cggtatcggt accgagactc gcggacgcgc gggcgtcgtc gtagaaaccg caacacagaa
     1081 gcccggcaaa cacagccacc agcaggcgca cgggcgtcag cttcgggggg ccagctggcc
     1141 aacgcgtgaa cggcaggtgc ccaacttcac agtcccgcgt gtcggttcgg cgcataagag
     1201 tccacccacc tgggaatcat tcgctacacg aagtagctca ttccagatgt actcccaggg
     1261 agatcagatg tcgaagtacc tgtatgcggt cggcggcttc agcttccgcc gtcgttggct
     1321 ggtgctcggc acctggctgg cgatcctcgt cggtgtcatg accgccggcc tcggtttggg
     1381 cggggaaccg agcgacgact tcagcattcc cggcaccgag tcgcagcgtg ccgtcgaggt
     1441 gcttgcgcag aaactgcccg cgttcagcgg cgcgcagacg cagatcacct tcgccgcccg
     1501 gggcggcgcc aggctgaccg atcccgccgt ctcgtccggc atcgaccagt ccatcgcgaa
     1561 tctgagatcg attccgcagg tcgccgtcgc cgcgggaccg gcgcagaccc ggctggtgtc
     1621 ccccaatggg caggtcggcc ttggcacggt gcagtggaag gctcaacccg gcgaggtcga
     1681 cgacccggca cttcaggcgg tcgaggccgc catgcggccc gctcaggcgg ccggtgtgca
     1741 ggtcgaatac tcgggcagcg tgtttcccgg ctacaaggtg agcgtccccg aactacccga
     1801 gatcatcggc atcatcgtcg ccttcggcat actgctggtc accttcggct ctgtggtcgc
     1861 cgccgggctc ccgatcgtca cggcgtgcat cggcgtcgga atcggcgcac tcggcatctt
     1921 cgccgtcgcg gcgctcgtcg acatgccgtc ggctgcgctg tccctcgcgt tgatgctcgg
     1981 cctgtcgtgc ggcatcgact atgcgctgtt catattgaac cggtaccgga acaatctgat
     2041 tctgctgaag cccaaggagg agtcggccgc gctggccgtc ggcaccgcag gcggcgcagt
     2101 ggtcttcgcc gcgttgacgg tgatcatcgc cctgtgcggg ctgtccatcg tcggcatacc
     2161 gtttctcacc tacatgggcg tggcggccgc ggcgtcggtc ctgatcgcct tgatgatcgc
     2221 ggtgacactc ctacctgcgc tgctcggctt cgccggacat cgcgtcgcga agttcatccc
     2281 gacgccgttc gaccgggcga aggagaccgc gcaggttgcg gccttcacgc cgcaccggac
     2341 gatgggggcc cggtggggac gcttcgtcgt cagcatgcgg aagccgctgc tgctcgcggg
     2401 taccgtcgca ctgcttgtgc tcgggctgcc cgcctttagc atgcacctcg gattgcccag
     2461 cggcgcttcg caaccggagt ccaacacgtc gcgcagggca tacgacctga cctcgcagca
     2521 cctgggcccg ggcttcaacg gcccgcttct cgtggtcgcc gacctcacca gggccaac
//
LOCUS       DASYLX010000867         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1477036,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000867 DASYLX010000000
VERSION     DASYLX010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477036"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGP27_20260"
     CDS             complement(<1..467)
                     /locus_tag="VGP27_20260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7853667.1"
                     /translation="MDEIRRIASREVYRNDWMTVREDQVRRPDGSDGIYGVVDKPWYA
                     LIIAYDRDRLRLVEQFRYPLGMRRWEFPQGTAPGRADLDPSELAHRELREETGLRAES
                     MVPVGLLDVAAGMSSQRGRVFVATGITEGDHEREHTEQDMHSEWFARSDVERM"
     gene            508..1920
                     /locus_tag="VGP27_20265"
     CDS             508..1920
                     /locus_tag="VGP27_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7853668.1"
                     /translation="MLLASLNPAAVAGGADLGDAVRIDGAVLSRSDLVGAATSVAERV
                     GGAQRVAVLARPTATTVLAVTGCLIAGVPVVPVPADVGAAERRHILTDSGAQLWLGEP
                     PEEPEGLPQIPVRLHARSWHRYPEPPPEATALIMYTSGTTGPPKGVVMSRRAIAADID
                     ALAEAWQWTSDDTLVHGLPLFHIHGLVLGLLGSLRIGNRFVHTGKPKPTLYAEAQGSL
                     YFGVPTVWSRVVDDLDVARALSSARLLVSGSAALPVPVFDRLAELTGHPPVERYGASE
                     TLITISTRAAGERRPGWVGLPLTGVRTRLVDDDGAPSPHDGETVGSLQIQTPTIFDGY
                     LNRADATAEAFDADGWYRTGDAAVIDGDGMHRIVGRESVDLIKTGGFRVGAGEIETVL
                     LAHPGVSEAAVIGVPDDDLGQRIVAFVVGDAEPRALIDYVAEELSVHKRPRQVRVVDS
                     LPRNAMGKVLKKGLAGEADK"
     gene            1917..>2056
                     /locus_tag="VGP27_20270"
     CDS             1917..>2056
                     /locus_tag="VGP27_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7853669.1"
                     /translation="MTLRFDEICIDAHDATALGAWWAGLLGWPQDIDDDGDVRLHPPP
                     GAG"
BASE COUNT          293 a          706 c          734 g          323 t
ORIGIN      
        1 atcatccgct cgacgtcgga gcgggcgaac cactcgctgt gcatgtcctg ttcggtgtgc
       61 tcgcgctcgt ggtcgccctc ggtgatgccg gtggcgacga acaccctgcc gcgctggctg
      121 ctcatgcccg ccgccacgtc gagcagcccg accgggacca tcgattcggc gcgcagccct
      181 gtctcctcgc gcaattctcg gtgcgcgagc tcgctcgggt cgagatcggc ccgcccgggc
      241 gcggtgccct gcgggaactc ccagcgacgc attccgagcg gataccggaa ctgctcgacg
      301 agccgtaacc ggtcgcggtc gtaggcgatg atcagcgcgt accacggctt gtcgacgacg
      361 ccgtagatgc cgtccgagcc gtcgggcctg cgcacctggt cctcgcgcac cgtcatccag
      421 tcgttgcggt acacctcgcg agacgcgatg cgccgtatct catccacgcg ccaagtatgc
      481 cgagcgccag cgggtagcct ctctgacgtg ctgctggctt cgttgaaccc cgcggccgtc
      541 gcgggcggcg ccgacctcgg cgatgcggtg cgcatcgacg gtgccgtgct cagccgcagt
      601 gacctcgtcg gcgcggcaac gtcggtggcc gagcgcgtcg gcggcgcgca gcgggtcgcg
      661 gtactcgcca ggcctaccgc caccaccgtg ctggctgtca ccggctgcct gatcgccggt
      721 gtgccggtgg tacccgtgcc cgcggacgtc ggcgcggccg aacgacggca catcctcacc
      781 gactccggtg cgcagctgtg gctgggggag ccgccggagg aaccggaggg gctgccgcag
      841 atcccggtgc ggttgcatgc gcggtcgtgg caccgctatc ccgagccgcc gccggaggcc
      901 accgcgctga tcatgtacac ctcggggacc acggggccgc ccaagggcgt cgtgatgagt
      961 cgccgggcca tcgccgcaga catcgacgcg ctggccgagg catggcagtg gacgtccgac
     1021 gacacactgg tgcacggact tcctctgttt cacattcatg gcctcgtgct cggcctgctc
     1081 gggtcgctgc ggatcggaaa ccgcttcgtg cacaccggaa aaccgaagcc gacgctgtac
     1141 gccgaagcgc agggctcgct gtacttcggg gtgcccacgg tgtggtcgcg ggtcgtcgac
     1201 gacctcgacg tagcacgggc gctgtcctcg gcccggttgc tggtgtcggg cagcgcggct
     1261 ctgccggtgc cggtgttcga ccggctcgcc gaactcaccg gccacccgcc ggtcgagcgg
     1321 tacggcgcca gcgaaacgct gatcaccatc agcacccgcg ccgccggcga gcgtcgaccc
     1381 ggttgggtgg gtctgccgct gaccggtgtg cggacccggc tcgtggacga cgacggcgca
     1441 ccgtcgccgc atgacgggga aaccgttggt agccttcaga ttcagacccc gacgatcttc
     1501 gacgggtatc tcaaccgcgc cgacgccacc gcggaggcct tcgatgccga cggttggtac
     1561 cgcaccggcg acgcggcggt catcgacggt gacggcatgc accgcatcgt cggccgcgaa
     1621 tcggtggacc tgatcaagac cggtggcttc cgggtggggg cgggagagat cgagacggtg
     1681 ctgctcgctc atccgggtgt gagcgaggcg gcggtgatcg gagtgcccga cgacgacctt
     1741 ggccagcgca tcgtcgcgtt cgtcgtcggc gatgccgagc cgcgtgcgct gatcgactat
     1801 gtcgcagagg agctttcggt gcacaagcgg ccgcggcagg ttcgtgtcgt cgacagcttg
     1861 ccacgcaacg cgatgggcaa ggtgctcaag aaggggttgg cgggggaagc cgataaatga
     1921 cgctgaggtt cgacgagatc tgcatcgacg cccatgacgc caccgccctc ggcgcgtggt
     1981 gggccgggct gctgggctgg ccgcaggaca tcgacgacga cggcgacgtg cggcttcacc
     2041 cgccgccagg cgcagg
//
LOCUS       DASYLX010000868         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_51125, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000868 DASYLX010000000
VERSION     DASYLX010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51125"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="VGP27_20275"
     CDS             <1..95
                     /locus_tag="VGP27_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853670.1"
                     /translation="EELAGAHISCIGVGPGREQTIVRRDILAGP"
     gene            92..748
                     /locus_tag="VGP27_20280"
     CDS             92..748
                     /locus_tag="VGP27_20280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7853671.1"
                     /translation="MTSARSEKDNDPRVVDPDYDRHGGFPNFEAAQPGPGFGRFLAAM
                     RRAQDLAVSADPDTDTWNEAADLAENLVKLLDPFEAPEGVGPANRVPALPGSGSLLMP
                     PYAVTKFESDGVELTVQFSRFHVGGNYAVHGGVLPLMFDSMFGMVIHASGRPISRTGF
                     LHVDYRRVTPIDTPLTMRGWVDRTEGRKAFVKAELRDPDGNLLAEANGLMIRLLPGQP
                     "
     gene            745..1404
                     /locus_tag="VGP27_20285"
     CDS             745..1404
                     /locus_tag="VGP27_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853672.1"
                     /translation="MTSLTPHPLTTPRAAALAGVLFGLLFGAALILIRTALPEGAEEG
                     AQWLDAGSHRMRIAAILMPFAGIAFLWFIGVVRDGFGRVEDKFFSTVFIGAGLTFLAM
                     MFVSSAIGAGLAASSRDVVDVSAHTEVATFGQMVLITLTKTYALRMGAVFMISLATMW
                     LKTGLMPRWLVIVTYVLALGILLGSDISMWMTLAFPIWVLIVSVLLLRRAGVIDLDRD
                     D"
BASE COUNT          231 a          470 c          516 g          290 t
ORIGIN      
        1 tggaagagct tgccggcgcc cacatttcgt gcatcggtgt gggacccggc cgcgagcaga
       61 ccatcgtgcg ccgcgacatt ctggcgggcc cgtgacttcc gcgcgcagcg aaaaggacaa
      121 tgatccccgc gtcgtcgatc ctgactacga caggcatggc gggttcccga atttcgaagc
      181 ggcccaacct ggtccagggt tcggcaggtt tctggcggca atgcgcaggg cgcaggatct
      241 cgcggtgtcc gccgaccccg acaccgacac ctggaacgaa gcggccgacc tcgcggaaaa
      301 cttggtgaag ctgctggacc cgttcgaggc gccggaaggt gtcgggcctg ccaaccgggt
      361 gcccgcgctg ccgggctcgg gcagcctgct gatgccgccg tatgcggtga cgaagttcga
      421 atccgacggc gtcgagttga ccgtgcagtt cagccgcttt cacgtcggtg gtaattacgc
      481 ggtgcacggc ggtgttctgc cgctaatgtt cgactcgatg ttcggcatgg tgatccatgc
      541 gtccggacgg ccgatcagcc gcaccggctt cctacatgtg gactaccgga gggtgacgcc
      601 gatcgacacc ccgttgacga tgcgcggttg ggtcgaccga accgagggcc gcaaggcgtt
      661 cgtgaaggcc gaactgcgag acccggacgg caacctgctc gccgaggcca acggcttgat
      721 gatccggctg ctgccggggc agccgtgacc agcctgacgc cgcacccgct gaccacccct
      781 cgcgcggcgg cgctggcagg cgtgttgttc ggactgctgt tcggggccgc gctgattctg
      841 atccgcaccg cgctgcccga aggcgccgag gagggcgcgc agtggctgga tgcgggcagc
      901 catcgaatga ggatcgcagc gatcctgatg ccgttcgcgg gcattgcctt tctgtggttc
      961 atcggcgtcg tgcgagacgg cttcgggcgc gtcgaggaca agttcttctc caccgtgttc
     1021 atcggtgcgg ggctgacgtt cctggcgatg atgttcgttt cgtcggccat cggtgcgggg
     1081 ttggccgcca gcagcaggga cgtcgtggat gtgtccgcgc acaccgaggt tgccaccttc
     1141 gggcagatgg tcctgatcac cttgaccaag acctacgcgc tgagaatggg cgccgtgttc
     1201 atgatctcgc tggccaccat gtggctcaag accggcctga tgccacgctg gttggtgatc
     1261 gtgacttatg ttctggcact ggggattctg ctcggcagcg atatcagcat gtggatgaca
     1321 ttggctttcc ccatctgggt gctcatcgtc agcgtgctgc tgctccgccg cgcgggcgtg
     1381 atcgacctgg accgcgacga ctagtcagtc gacgatcgtc agcgcctgcg acgggcagag
     1441 cttgaccgcc tcgcgcgcct tgtcttgctc gtcgtcgggg atgtcgtcgg cgagcaccac
     1501 cacgatg
//
LOCUS       DASYLX010000869         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3890233,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000869 DASYLX010000000
VERSION     DASYLX010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890233"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VGP27_20290"
     CDS             <1..913
                     /locus_tag="VGP27_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEV7853673.1"
                     /translation="EAVAETLVDDGLLRRRPSGYFPSPGVDPHPAVDIRGSSGGQIAI
                     LETETGRLLGSADVGRAPATVHPGAVYLHQGETYLVDSLDFEDGVAFVHADDPGYTTF
                     ARELTDIAVTGPGERNTFGPITIGLVPVSVSNTVIGYLRRERNGEVIDFVELEMPTRT
                     LDTKAVMCTITPEALQDNGVEPLRIPGALHAAEHAAIGLLPLVASCDRGDIGGLSTAV
                     GPVAGLPTIFVYDGYPGGAGFADRGFQQIATWWEATAAAIEACECPLGCPSCVQSPKC
                     GNGNDPLDKAGAVRVLRLVLRELGRQE"
     gene            1204..2307
                     /locus_tag="VGP27_20295"
     CDS             1204..2307
                     /locus_tag="VGP27_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7853674.1"
                     /translation="MSHDWLLVETLGSEPVVVADGRQAKNLIPVSVFLRRSPDLMAIQ
                     TAISESVHSGEAMSTITPRNDRVIRTEPVVMSDGRVHGVHVWIGPLGMEPPERTMPGP
                     LKWDLTAGVATDTPESLYNSGRDPSTEATHGRTFAEDLPMRTLNPNETKVLSMAIKAE
                     PGMKLCSTWNVTDHKGETITVGFVARANPETQDDGRERLICRAMNWRAQFDGPTLPPD
                     HLAQRILDGLPCPGVHRALVDPVNWTLLKWLDEPAPFFDWHARENGKESANPADALQM
                     ARMALEYANGATSGVLRLRTYDGGWTPVHVTINRVELEPDTFAALMTLRVPTDDELAV
                     MQFAETEEAASPDKKSRKGKKNKKDKKKSEDDG"
     gene            complement(2304..2630)
                     /locus_tag="VGP27_20300"
     CDS             complement(2304..2630)
                     /locus_tag="VGP27_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEV7853675.1"
                     /translation="MWDEHGSATVIAVAMIAALLAVTLGGSYLGSAVVARHRAQAAAD
                     LAALAAAARLPAGAESACGQASVIAHAMEVEVADCVVDDLDVVITIRVGQARAAARAG
                     PVDGPS"
     gene            complement(2620..2952)
                     /locus_tag="VGP27_20305"
     CDS             complement(2620..2952)
                     /locus_tag="VGP27_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEV7853676.1"
                     /translation="MTGDRGASTVEAAFAIAALVVVLVLCVAGLTTISMQVRCIDAAR
                     EAARLAARGDDGEAEQAAHSIAPLDAHLDLRREGRLVFATVTARSILLPGVAIRADTV
                     AVVEPTVG"
     gene            complement(2949..3152)
                     /locus_tag="VGP27_20310"
     CDS             complement(2949..3152)
                     /locus_tag="VGP27_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEV7853677.1"
                     /translation="MMGNVIRKLKVRMALLLVDDTGMSTVEYAIGTIAAAAFGAILYT
                     VVTGDSIVTALTNIINRALNTNV"
     gene            complement(3195..3734)
                     /locus_tag="VGP27_20315"
     CDS             complement(3195..3734)
                     /locus_tag="VGP27_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV7853678.1"
                     /translation="MLVAGSPSRARIRVAVPTPAVAVSLPGDSDALAVASTLDVFAAC
                     LSSGMAVSSAAAAAAPSAPRLLSTVLARAADMLALGADPVAAWSNSDLPLDVHGEALL
                     RLARRSATSGAALAQGVSDLADQSRHDAASAADARAQRASVLIAGPLGLCYLPAFVCL
                     GIVPIVAGLAGDVLRSGLA"
BASE COUNT          713 a         1242 c         1226 g          560 t
ORIGIN      
        1 cgaggccgtc gccgagaccc tggtcgacga cgggctgttg cgccgacgtc cgagtggtta
       61 cttccccagc ccaggcgtcg acccccatcc cgcggtcgac atccgcggct cgtccggggg
      121 ccagatcgcg attctggaaa ccgaaacggg acggcttctg ggtagcgcgg acgtcggccg
      181 tgcgcctgcc accgtccatc ccggggcggt gtatctgcac caaggcgaga cgtacttggt
      241 ggactcgctc gacttcgagg acggcgtggc gttcgtgcac gccgacgacc ccggatatac
      301 gacgttcgcg cgagagctca ctgacatcgc ggtgaccggg ccgggcgagc gaaacacgtt
      361 cggcccgatc accattggcc tggtgccggt gtcggtgagc aatacggtga tcgggtatct
      421 gcgccgcgag cggaacggcg aggtgatcga tttcgtcgag ctcgagatgc cgacccgaac
      481 tttggacacc aaggcggtca tgtgcacgat cactccagag gcattgcagg acaacggagt
      541 cgaaccgctg cgcattcccg gtgccctgca cgcggccgaa cacgcggcga tcggacttct
      601 tccgttggtt gccagttgcg accgcggcga catcggcggg ctgtccacag cggtggggcc
      661 tgttgccggg ttgccgacga ttttcgtcta cgacggttac ccgggtggtg cagggttcgc
      721 cgatcgcggc ttccagcaga tcgctacctg gtgggaagcc acagcagccg cgatcgaggc
      781 ttgcgaatgc cccctcggct gcccctcctg tgtgcagtca ccgaagtgtg gcaacggaaa
      841 cgaccccctg gacaaggcgg gcgccgttcg cgtgctgagg ctggtactgc gcgagctggg
      901 gcgtcaggaa taggaacacg ggcgacggcc gacccctcga cggccgcccc cgtggaccag
      961 ctatcgacgg tgccggatcg ggcgcgccga gcgccagacg gttgggacca aacgctcaca
     1021 acgcaattcg ttgttgtccc ggtaagtcag gcgtagacgt aaattacctc gtgggagccc
     1081 tttaggcaac ctgagctcgc attttcggtg ccgtcaaata agtggtctat accaagatgc
     1141 tacggatttc gttgccattt ccgcctgcgg gtgcgtaaag cgcagaccgg taagatttgc
     1201 cccatgagcc acgactggct gctggtggaa accctcggca gcgagcccgt tgtggtcgcc
     1261 gatggccgtc aagccaaaaa tctcattccg gtcagcgttt ttctgcgacg aagtccggac
     1321 ctgatggcga ttcagaccgc gatatccgaa tctgtgcact caggcgaggc catgagcacg
     1381 atcacgccgc gcaacgaccg cgtaatccgt accgagccgg tggtgatgtc ggacggccgg
     1441 gtgcacggcg tgcacgtctg gatcgggccg ctgggcatgg agccgccgga acggacgatg
     1501 cccggcccat tgaaatggga tctgaccgcc ggcgtcgcca ccgacacacc ggagtcgctc
     1561 tacaacagcg gccgggaccc gtcgacagaa gccacccatg gcaggacgtt cgccgaagat
     1621 ctgccgatgc gcaccctcaa tcccaacgag acgaaggtgt tgtcgatggc catcaaggcc
     1681 gaacccggca tgaagctgtg cagcacgtgg aatgtcaccg accacaaggg cgagacgatc
     1741 accgtcggat tcgtcgcccg cgcgaatccg gaaactcagg acgacggtcg cgagcgactc
     1801 atctgccgcg ccatgaactg gcgggcgcag ttcgatggtc ccacactgcc ccctgatcac
     1861 ctcgcgcagc gaatactcga cgggcttcca tgtcccggtg tgcaccgggc gttggtcgac
     1921 cccgtgaact ggacactgct gaagtggctc gacgagcccg ccccattctt cgactggcac
     1981 gcgagggaga acggcaagga gtcggccaac cccgcggacg cgcttcagat ggcgaggatg
     2041 gcgctcgaat atgccaatgg agccaccagc ggcgtgctgc ggctgcgtac ctacgacggc
     2101 ggatggacgc cggtacacgt caccatcaac cgcgttgagc tggaaccgga taccttcgcg
     2161 gcgctgatga cgctgcgcgt gccgaccgac gacgagctcg ccgtcatgca gttcgccgag
     2221 accgaagaag ccgcttcgcc ggacaagaag tcgcgaaaag gtaagaaaaa caagaaggac
     2281 aagaagaagt ccgaggacga cggctaactc ggcccgtcga ccggcccagc ccgtgcggcg
     2341 gcacgcgcct gcccgactcg gatggtgatc accacgtcga ggtcgtcgac cacacagtcg
     2401 gccacctcga cttccatcgc atgcgcgatc accgatgcct ggccgcaggc tgactcggca
     2461 cccgccggta accgcgccgc agccgccaac gcggcgaggt cggcggccgc ctgagcgcga
     2521 tggcgcgcaa cgaccgccga ccccaggtaa gacccgccga gggtgacagc gagcagcgcg
     2581 gcgatcatcg cgacggcgat caccgtggcc gaaccgtgct catcccacag tcggttcgac
     2641 cacggccacc gtgtccgccc gaatcgcgac gcccggcaac aagatcgagc gagccgtgac
     2701 ggtagcgaaa accaaccggc cttctctgcg gaggtcgagg tgtgcgtcga ggggagcgat
     2761 tgagtgcgcg gcttgctccg cctcaccgtc atcgccacgc gccgcgagtc gggccgcctc
     2821 ccgcgcggca tcgatacacc gcacctgcat cgatatcgta gtgagcccgg caacgcacag
     2881 caccagaacc accaccagtg cggcgatggc gaatgccgcc tcgacggtgc tggcaccacg
     2941 atcaccggtc aaacgttggt gttcagcgcg cggttgatga tgttcgtcaa cgcggtcacg
     3001 atggagtcgc cggtgacgac cgtgtagaga atcgcgccga acgccgcggc ggcgatcgtg
     3061 ccgatggcgt actcgacggt cgacatgcct gtgtcgtcga ccagcagcag cgccattcgc
     3121 acctttagtt tgcgaatcac attgcccatc aatacttttc ccttccatcg aatattcgga
     3181 cagttcgcgg gaggtcatgc cagacccgac cgcagtacgt cacccgccag gcccgcgacg
     3241 atcggcacga tgccgaggca cacgaaggca ggcagataac acaagccgag cggcccggcg
     3301 atcagcaccg aggctcgttg cgccctcgca tcggcggccg acgccgcatc gtggcgcgac
     3361 tggtcggcca ggtcggaaac gccctgggca agtgcagcgc ctgacgtggc agaccgccgg
     3421 gccagccgaa gaagggcctc cccgtgcacg tcgaggggca gatcggaatt cgaccacgcc
     3481 gcaacaggat ccgcacccag cgcaagcatg tccgcggccc gggcgagcac cgtggaaagc
     3541 aggcgcggcg ccgatggcgc ggcagcggcc gccgcggaag acaccgccat gcccgagctt
     3601 aggcaggcgg cgaacacgtc tagcgtggat gcgacggcca gcgcatccga gtcgcccggg
     3661 agcgaaaccg ccaccgccgg ggtgggcacc gccacgcgga tccgggcccg tgacgggctg
     3721 ccggcaacca gcagcgctgt c
//
LOCUS       DASYLX010000870         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_820139,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000870 DASYLX010000000
VERSION     DASYLX010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820139"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VGP27_20320"
     CDS             <1..1037
                     /locus_tag="VGP27_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853679.1"
                     /translation="AITEAVDSDLVRGVMATDALIGTFARLDDASLQQNICFLYHLLG
                     GGTGEWDVPIGGMGAVSGALAAAASGFGAEITTNAEVYRIDPDGEVCYLLDGQEHRVH
                     ADHILAGVTPTVLAELVGDPAPSAAPGAQVKVNLLLRRLPRLRDDTVTPEEAFGGTFH
                     INENYSQLEAAYSRATTGSIPDPLPCEIYCHSLTDPSILSDELRASGTQTLTVFGLHA
                     PHDLATGADPDRLREDLTSAVLHSLNSVLAEPIQDVLMEDASGRLCIEAKTTQDLEEI
                     LGMTSGNIFHGSLNWPFVEDDEPLDTPARRWGVATEHDRILLCGSGSRRGGAVSGLGG
                     HSAAMAVLES"
     gene            complement(1170..4295)
                     /locus_tag="VGP27_20325"
     CDS             complement(1170..4295)
                     /locus_tag="VGP27_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7853680.1"
                     /translation="MAISCGACGAGLRDGARFCDTCGSPVAADSHAEYKQVTVLFADV
                     VHSMDIAAAVGPERLREIMGEVFRRSSVIVQRYGGTVDKFTGDGIMAVFGAPIALEDH
                     ALRACRAALDIQTDAQHLAAEVQRRDDASLQLRIGLNSGEVITGEIGTGPMSYTAIGE
                     QVGMAQRMESIAPRGGVMMSDSTARLVDTSAMMGETELVHIKGARDPVPARRLFGMST
                     GRRKDRVEPTFVGRQWELDSLQRLFDRAVSGQGCVVGVVGPAGIGKSRIVRELTSLAD
                     GSGIEVSATYCESHTAEVAFHAAAGLVRSTTGLDGLDGASARKEVRERFAGADEEDLL
                     LIDDLLGIADPEAELPQIDPDARRRRVAAMVKAVALARISPVTYVIEDAHWIDGVSES
                     MLADFLSVIPATPSLVIITYRPEYDGLLAHSPHSQTIALEPLDENQMLALGTELLGGD
                     RSVTELAELIAERAGGNPFFAEEIVRDLSERDVLVGGRGCYLCTGPAADVHVPSTLQA
                     VIAARIDRLGPAAKRTLNAAAVIGSRFTSDMLKVLEIPAALDDLISAELIDQTATTPQ
                     HEYSFRHPLVRAVAYESQLKSARAQLHRRVATAIEQSDQNGALIAEHLEAAGDLLAAY
                     EWHMKAGAWSAHRDISAAQLSWERALQVADELPADNPNRMAMRIAPRTLICGNMFKRF
                     HPDISSRFEELRDLCTEADDKASLAVGMAGMTVEHVLHGRVQDASTLASETMALVESI
                     GDPALTIALSFAGIVAKHETGETVDALRWTQAVIDLTARDFDKGRLIFESPRATALAW
                     RGVARFRTGAPGFHEDFGEALAIAEQSDTLSKSTIITYKYAGIPRGVFLADDTVLREI
                     EAALKSVERSSDDMALFLLRLTFAIALIYHGADRSRGYELMRSLRETSIKERFAMNML
                     PVAETYLARERAEQGEEDLAIGQWRSLAEEMDRAGHYNNIDIPLIYTAQQLMSRGDYD
                     EAACEIERLMSLAADHQWASREIAALQLRAELALARGDETAYRELRDRYRAMANGLGF
                     EGHMKWAAGLP"
     gene            complement(4404..4820)
                     /locus_tag="VGP27_20330"
     CDS             complement(4404..4820)
                     /locus_tag="VGP27_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7853681.1"
                     /translation="MSGNALADEVWRAMASLVNDNRDSWKRAVIERTGLPFSRIRILR
                     RLARQPMTVKDIAQAATVDAPAATVAVNDLESRGLVVRETDPANRRCKVVSLTDAGRE
                     TVAAMRSVEDPAPEILSALDEAELRKLQAILAKLAR"
     gene            complement(4810..>5395)
                     /locus_tag="VGP27_20335"
     CDS             complement(4810..>5395)
                     /locus_tag="VGP27_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853682.1"
                     /translation="MMSLYLQGQRGFSAMHTGLIYLPVAVGALIFSPLSGRLVGRFGS
                     RPSLFVAGILITAATLMLTQLTATTAVWQLLVVFAVFGVGFSMVNAPITTAAVSGMPT
                     DRAGAASAVASTSRQVGVSLGVALCGSVAGAALATAGADFAVASQPLWYICAALGVAI
                     FVLGIFSTSARATRSAQRLAPLIDQTQWEGVRVR"
BASE COUNT          934 a         1805 c         1724 g          932 t
ORIGIN      
        1 gcgcgatcac cgaggccgtc gacagcgatc tggtccgcgg ggtgatggcc accgacgcgt
       61 tgatcggcac gttcgcccgt ctcgacgatg cgtcgctgca gcagaacatc tgctttctgt
      121 atcacttgtt gggcggcggc accggcgagt gggatgtgcc catcggcgga atgggcgcgg
      181 tcagtggagc gctggccgcg gccgccagcg gctttggcgc cgaaatcacc accaacgcag
      241 aggtttacag gatagatcct gatggtgagg tgtgttacct gctcgacggc caggaacacc
      301 gtgtgcatgc cgaccacatt ctggcgggtg tgacaccgac ggtgctggcg gagttggtcg
      361 gcgacccggc gccgtccgca gcgccgggtg cccaggtgaa ggtgaacctg ctgttgcgac
      421 gtctaccgcg acttcgagac gacaccgtca cgcccgaaga ggctttcggc ggcaccttcc
      481 atatcaacga gaactacagc caactcgagg cggcatactc acgcgcgacg accggctcca
      541 tacccgaccc cttaccgtgc gagatctact gtcactcgct gaccgatccg tccatcctgt
      601 ccgatgaact gcgagcgtcc ggcacacaga ccctcacggt cttcgggctg cacgcgccgc
      661 atgacctggc gaccggcgcg gatcccgatc gactgcggga agacctgaca tcggcggtac
      721 tgcattcgct caattccgtt ctggccgaac cgatccaaga tgtattgatg gaggatgcgt
      781 cgggtcggct gtgtatcgag gccaagacga ctcaggatct ggaggaaatt ctgggtatga
      841 cgtcgggcaa catcttccac ggctcgttga actggccgtt cgtcgaggac gacgaaccgc
      901 tggacacgcc cgcccgtcgc tggggcgtgg cgaccgagca cgatcggatt ctgttgtgcg
      961 gctcgggttc acgtcgcggc ggagcggtgt ccggcctggg cgggcacagt gcggcgatgg
     1021 cggtattgga gagctgaatc cttccgtacc cgtacccgta cccgtacccg tacccgtacc
     1081 cgcggggcct accgccgacg gcaccgcgag cgtgcgtgtt tgcgcccgac acgccgcgat
     1141 aatgtggcag tctgcgcacg ctcgcgacgt tagggcaacc ccgcggccca cttcatgtgg
     1201 ccctcgaagc cgagcccgtt tgccatggcg cggtagcggt ctcgcagttc ccggtaagcg
     1261 gtttcgtcgc cgcgcgccag cgccagttca gcgcggagct ggagcgccgc gatttcgcgt
     1321 gaggcccatt ggtgatccgc cgccaacgac atgagccgct caatctcgca ggcggcttcg
     1381 tcgtagtcgc cccgcgacat cagctgttgc gcggtataga tcaacgggat gtcgatgttg
     1441 ttgtagtgac cggccctgtc catctcctcg gccaacgagc gccactgccc gatcgcgagg
     1501 tcttcttcac cctgctctgc ccgctcccgg gctaggtagg tctcggcgac gggaagcatg
     1561 ttcatcgcga agcgttcctt gatacttgtt tcgcgcaggc tgcgcatgag ttcgtagccg
     1621 cgtgagcgat ccgcgccgtg gtagatgagg gcaatcgcaa acgtcaggcg cagtaggaac
     1681 aacgccatgt cgtcgctcga ccgctcaact gacttcagtg ctgcttcgat ctcgcggagc
     1741 actgtgtcgt ccgccaggaa aacgcctcgc ggtatgcccg cgtacttgta ggtgatgatg
     1801 gtcgacttgg acaatgtgtc ggactgctcc gcaatggcaa gcgcttcacc gaagtcctcg
     1861 tgaaagccgg gcgcacctgt ccggaatcgg gccactcccc gccacgccaa cgccgtggca
     1921 cgtggggatt cgaagatgag cctgcctttg tcgaaatctc gggcggtcaa gtcgattacg
     1981 gcttgggtcc aacgcagcgc atcgaccgtc tcacccgtct cgtgcttggc gacgattcct
     2041 gcgaacgaca gcgcgatggt cagcgcgggg tcgccgatcg attcgaccaa cgccatggtc
     2101 tccgacgcca gcgtggacgc atcctgaaca cgtccgtgca gcacatgttc gaccgtcatg
     2161 ccggccatcc ccaccgccag cgacgccttg tcgtcggcct cggtgcacaa gtcccgcaac
     2221 tcctcaaagc gcgacgagat gtcggggtgg aacctcttga acatgtttcc gcatatcagc
     2281 gtgcgtggcg cgatgcgcat ggccatccgg ttcgggttgt cggcgggtag ttcgtcggcg
     2341 acttgcagtg cacgctccca gctgagttgc gccgcggaaa tgtcgcggtg ggccgaccat
     2401 gcgccggcct tcatatgcca ctcataggcg gcaagcagat cgcccgccgc ctccaaatgc
     2461 tcggcgatca atgcgccgtt ctggtcgctc tgttcgatcg cggtcgcgac cctacgatgc
     2521 aattgcgcac gcgcggattt gagctgcgac tcgtacgcga cggcgcgtac gagcggatgc
     2581 cgaaaggaat attcgtgctg aggggtggtc gctgtctggt cgatcagctc cgcggaaatc
     2641 agatcgtcca gagccgctgg gatctcgagg actttcagca tgtccgacgt gaatcgcgac
     2701 ccgatcaccg ccgcagcgtt cagcgttcgc ttggccgcgg ggccgaggcg gtcgatgcgc
     2761 gcggcgatca ccgcctgcag tgtgctcggc acgtgcacgt ctgccgccgg accggtgcac
     2821 agatagcaac cgcgaccgcc gaccaagacg tcgcgctcgc tgagatcccg gacgatctct
     2881 tcggcgaaga aggggtttcc gccagcgcgc tcggcgatga gctcggccaa ctcggtaacc
     2941 gatctgtccc cgcccagcag ctctgtgccc aacgccaaca tctggttttc gtcgagcggt
     3001 tcaagggcga tcgtctgcga gtgaggtgaa tgggccagca agccatcgta ttccggccga
     3061 tacgtgatga tcaccagtga cggggtcgca ggaatcacgc tgaggaaatc cgccagcatg
     3121 gattcgctga ccccgtcgat ccaatgcgcg tcttcgatga cgtaggtgac cgggctgatc
     3181 ctggccagcg ccacggcctt gaccatcgcc gccacccggc gtcgacgagc gtcgggatcg
     3241 atctggggca gttcggcctc gggatcggcg atgccgagga ggtcatcgat gagcaggaga
     3301 tcctcctcgt ccgcccccgc aaatcgctct ctcacctcct tccgcgcaga agcgccgtcc
     3361 agaccgtcca aaccggtggt ggatcgcacc aggcctgcgg cggcatggaa ggccacctcg
     3421 gcggtgtgcg actcgcagta ggtggcggac acttcgatac cggaaccgtc ggcgagcgac
     3481 gtcagctcgc ggacgatgcg gctctttcca atcccagccg ggccgaccac gccgacaacg
     3541 catccttggc cgctgaccgc gcggtcgaaa aggcgctgga gcgagtcgag ctcccactgg
     3601 cgaccaacga acgtaggctc gacacggtcc tttcgccgac cggtggacat cccgaacagc
     3661 cggcgagccg gcaccgggtc tcgagcaccc ttgatgtgca caagctccgt ctcgcccatc
     3721 atcgcgctgg tgtccaccag gcgtgccgtc gagtcgctca tcatcacgcc gccgcgcgga
     3781 gcgatcgact ccatccgttg cgccatcccg acctgttcgc cgatcgcggt gtacgacatc
     3841 ggaccggtcc cgatctcacc ggtgatcacc tcgccggagt tcaacccgat ccgcaactgc
     3901 agggacgcgt cgtcgcgtct ttgcacctca gccgccaggt gctgcgcgtc ggtctggatg
     3961 tccagtgccg cgcggcacgc tcgcagggcg tggtcctcga gtgcgatcgg cgcgccgaag
     4021 accgccatga tgccgtcgcc ggtgaacttg tccaccgtgc cgccatagcg ttgcacgatc
     4081 accgacgacc ggcgaaacac ctcgcccatg atttcgcgaa gccgttccgg cccgacggcc
     4141 gccgcgatgt ccatcgagtg caccacgtcg gcgaacagca cggtgacctg cttgtactcg
     4201 gcgtggctgt cggcggcgac gggcgaaccg caggtgtcgc agaatcgggc gccgtccctc
     4261 agcccggcgc cacacgcacc gcacgatatg gccaccacct catcgtgccg tcgaaagaca
     4321 agcctgggct gttaatcgct gagttggcga ccgggaatgc ccccgcaagc gggaggtgcc
     4381 ctgctcgcgg ggagtgcccc ctattagcga gcgagcttgg cgaggatagc ctgcagcttc
     4441 ctcagttcag cctcgtcgag ggccgacagg atctccggcg cggggtcctc gacggagcgc
     4501 atggctgcca ccgtctcgcg gcccgcatcc gtcaacgaca ccaccttgca ccgccggttg
     4561 gccggatcgg tctcgcgaac gacgaggccg cgactctcga gatcgttgac cgcgacggtg
     4621 gccgcggggg cgtcgacggt cgcggcctgc gcgatatctt tgaccgtcat cggctgccgg
     4681 gccagccttc tcagtatccg aatgcggctg aagggcagcc ccgtccgctc gatcacggcg
     4741 cgcttccagc tgtcacggtt gtcgttgacg agcgacgcca tcgcccgcca aacctcgtcc
     4801 gccagtgcgt taccggacac ggacaccctc ccactgcgtt tgatcgatca acggcgcgag
     4861 gcgttgcgcc gaacgggtgg ctcgggcaga cgtggagaag atgcccagta cgaagatggc
     4921 gacaccgagg gctgcgcaga tgtaccacag cggctgggag gccaccgcga aatccgcgcc
     4981 ggccgtcgcc agtgcggcac cggccaccga accacacagc gcgacaccga gactgacgcc
     5041 gacctggcgg cttgtcgacg ccaccgccga ggccgcaccc gcgcgatcgg tcggcatgcc
     5101 gctgaccgcc gccgtggtga tcggtgcgtt caccatcgag aacccgacgc cgaacaccgc
     5161 gaagaccacc agcagttgcc acaccgccgt cgtcgcggtc agttgggtga gcatcagcgt
     5221 cgcggcggtg atcaagatac ccgcgacgaa caatgacggc cggctgccga atcggccgac
     5281 cagtctgccg gaaagcggcg aaaagatcag tgcgccaaca gcaacgggca gatagatcaa
     5341 gccggtgtgc atcgccgaga acccgcgctg gccctgcagg tacagcgaca tcatg
//
LOCUS       DASYLX010000871         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2904485,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000871 DASYLX010000000
VERSION     DASYLX010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2904485"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGP27_20340"
     CDS             complement(<1..449)
                     /locus_tag="VGP27_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853683.1"
                     /translation="MVAPGFIAIGSGPAGLTAAETFRRKHPKIPVRILASDPALPYAK
                     PPLSKAYLCGRNPELDLHSRAWFVQNAIQLHHGITVDHIDLKNREVVTAAGRRYGYWH
                     LVLASGATPVPLAVPGGEAALSLRSFADAVVLRMAARNSDSAVVVGGG"
     gene            complement(513..890)
                     /locus_tag="VGP27_20345"
     CDS             complement(513..890)
                     /locus_tag="VGP27_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7853684.1"
                     /translation="MIGNLRSVVLDTKDPAKLAKFYGDVLGGTIQDVDETWSVVTDPQ
                     GRRLAFQLSPEHEPPRFPDPRGSQQFHLDIAVDDIDSAEREVLALGATRVTDAEGEDR
                     FRVFRDPAGHTFCLVWGITPTDV"
     gene            909..1847
                     /locus_tag="VGP27_20350"
     CDS             909..1847
                     /locus_tag="VGP27_20350"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEV7853685.1"
                     /translation="MPDPQTVTCSCFRRFPVGVHNFCTFDEAVGPASGLWDHLRMNPE
                     DDPEARIRELERPLNDQAQASELGGGQPGSNAYLPPPTTPYTAPDYNTPAYGNQPPYG
                     TQPYGNQQPYGAQPYGAQPYGAQPYGAQPYGNQPYNAPYGEPPRKSSGGIPWVVFGLI
                     AVLFVGGIIAGAVVYTMSNSGGSSVSSSDGSINIPTFPSIEIPNMPSIPAPPGAPDTD
                     SNVLIGVPGQDLTVSGIDEKKTITCNDANVIVSGIRNTVNITGHCVKVGVSGMNNIVT
                     VDAADAIGASGFDNRVTFHSGSPDIDAPGSNVVEQG"
     gene            1947..>2531
                     /locus_tag="VGP27_20355"
     CDS             1947..>2531
                     /locus_tag="VGP27_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853686.1"
                     /translation="MSDNGSAPAEPAGHSRLAVVVTWLVSAGIPLLAIGFGSWLLAAN
                     FRAAGPEFLLRYVFGERDTEPLTRGRDVANAADPQADLASDIHDVISRDWFVITGYFL
                     ILFACSLIFVLIMLAFSNSARTLSFAIFGAVIVATVMDIAENVLVLMATNPPAFPNRL
                     HDEFWIVVPAAVATVKWCALIVAMCAVPAAIFAVW"
BASE COUNT          441 a          828 c          814 g          448 t
ORIGIN      
        1 ccgccgccga ccacgaccgc cgaatcggaa tttctggcgg ccatccgaag gacgacggcg
       61 tcggcgaacg accgcaggga caacgccgcc tcgccgccgg gaaccgccag cgggaccggt
      121 gtcgcgccgg aggccagcac caggtgccag tagccatagc gccggcccgc cgcggtgacg
      181 acttcgcggt tcttcagatc gatgtggtcg acggtgatgc cgtggtgcag ttggatggcg
      241 ttctgcacga accaggcacg gctgtgcagg tccagctcgg ggttgcggcc acagaggtag
      301 gccttgctca acggcggctt ggcgtagggc agtgcggggt cgctggcgag gatgcgaacc
      361 gggatcttgg ggtgcttgcg gcggaacgtt tcggcagcag tcaaccctgc cggaccactg
      421 ccgatggcta tgaaacctgg tgccaccaca cttcagtggt accaccgcgg cggctttgct
      481 actccaaacg gttagctttg gtcacaacca ggtcagacat cggtgggcgt gatgccccac
      541 accaggcaga acgtgtgccc ggccgggtct cgaaacaccc ggaaccggtc ttcgccttcg
      601 gcgtcggtga ctcgcgtggc gcccagtgcc agcacctcgc gctccgcgct gtcgatgtcg
      661 tcgaccgcga tgtcgaggtg aaactgctgc gagccacggg ggtccggaaa tcgcggcggc
      721 tcatgctcgg gcgacagctg aaacgccagc cgcctgccct gtgggtcggt aaccaccgac
      781 caggtctcgt cgacgtcttg aatggtgccc ccgagaacgt caccgtagaa cttcgcgagc
      841 ttggcgggat ccttggtgtc gagcaccacg gagcggagat ttccgatcat gaggtcggtg
      901 ttgccatctt gccggatcca caaacggtta cctgcagttg tttccgccgc ttcccggtag
      961 gcgtgcacaa cttctgcacg ttcgacgaag cagtcggtcc cgcgtccgga ttgtgggacc
     1021 atctgcgcat gaatcccgag gacgaccccg aggcccggat cagggaactc gagcgcccgc
     1081 tcaacgatca ggcgcaggcg agcgaactcg gtggcgggca gccggggagc aacgcgtacc
     1141 tgccgccgcc gacgacgcca tataccgcgc ccgactacaa cactccggcc tacggcaacc
     1201 agccgccata cggcacgcag ccctacggca accagcagcc ctacggggcg cagccctacg
     1261 gggcgcagcc ctacggggcg cagccctacg gggcgcagcc ctacgggaac cagccgtaca
     1321 acgcgccgta cggtgagccg ccgcggaaat cctccggcgg catcccctgg gtggtgttcg
     1381 ggctgatcgc ggtgctcttc gtcggtggga tcatcgccgg cgccgtggtt tacaccatga
     1441 gtaacagtgg cgggtcgagc gtctcgagta gcgacggatc catcaacatt ccgacgtttc
     1501 cgtcgatcga gataccgaac atgccgagca tcccggcgcc accgggggca ccggacaccg
     1561 attcgaacgt gctgatcggc gtgcccgggc aagatctcac cgtctcgggc atcgatgaga
     1621 aaaagaccat cacctgcaac gacgcgaacg tgatcgtcag cggcatccgc aacaccgtga
     1681 acatcaccgg gcactgcgtg aaggtcggcg tctccggcat gaacaacatc gtcaccgtcg
     1741 atgccgcgga cgcgatcggc gcgtcggggt tcgacaaccg ggtcaccttt cactccggtt
     1801 cgccggacat cgacgccccc ggatccaatg tggtggagca gggctgacgg tgaccctcgg
     1861 cccgtagcga ttgtgcgcaa tgcgccaata atcgcaacgg aagttgtcag actggcgaca
     1921 cgaggatttc gtcgctgggg gccacaatgt ctgacaacgg ttctgcgccc gccgaacccg
     1981 cgggtcactc ccgtcttgcg gtagttgtca cctggttggt aagtgcaggc atcccgctat
     2041 tggcaatcgg attcggaagc tggctgctgg cagccaactt ccgcgccgcc ggtccggaat
     2101 tcctgttgcg ctacgtattc ggcgagcgcg acacggagcc gctgacgcgc gggcgggacg
     2161 tggccaatgc cgctgatccg caggccgatc tggcctccga cattcacgat gtgatttcgc
     2221 gggattggtt cgtcatcacc ggctatttcc tcatcctctt cgcgtgctcg ctcatcttcg
     2281 tcctgatcat gctcgcgttc tcgaattcgg cgagaaccct cagcttcgcg atcttcggag
     2341 ctgtcatcgt cgcgaccgtc atggacatcg cggagaacgt cctggttctg atggcgacga
     2401 atccgcctgc ctttcccaac cggctgcacg acgaattctg gatcgtcgtt cccgccgcgg
     2461 tcgcgacggt gaagtggtgc gcgctcatcg tcgcgatgtg cgcggtgccc gcggcgatat
     2521 tcgccgtctg g
//
LOCUS       DASYLX010000872         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1257257,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000872 DASYLX010000000
VERSION     DASYLX010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257257"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..541
                     /locus_tag="VGP27_20360"
     CDS             125..541
                     /locus_tag="VGP27_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853687.1"
                     /translation="MSDSADDDAAGDDLRRYAFHMDPQVTLTGDTWTASYPGADWSVS
                     GPSSQEALRRLGEEFVRRQNDGDDPLAYADQLYRRHLREPVPGVYAVDNELYRELVHA
                     PSAERQRTVKEAERRRRLGQSYTLLDYQREQGTSPT"
     gene            complement(542..862)
                     /locus_tag="VGP27_20365"
     CDS             complement(542..862)
                     /locus_tag="VGP27_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV7853688.1"
                     /translation="MSETKITVIYDNPIDPDAFEAAYESEQLEAARRIPGHIRFETSK
                     VWPKEDGSPTPAYRSIDLYYPDYSAASDAVTTPEAGAFFEAMARLSTGGVRVLFSDIE
                     VPQS"
     gene            complement(945..1352)
                     /locus_tag="VGP27_20370"
     CDS             complement(945..1352)
                     /locus_tag="VGP27_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853689.1"
                     /translation="MGKRRKTIAGALLSAGVALVGIGLGVGTAQALPGAPVPLGAWPG
                     CPEDNPSGPCNWCPGDPPVQTGNLRVNPVVWDNNACHTYWYVYHGQGNVASNIFEGEA
                     PPAPPPPPPNLTPPLPPGWCWSLFIPTTSCPNG"
BASE COUNT          284 a          533 c          552 g          303 t
ORIGIN      
        1 aggccgaaag gggcattcgg cttctacaga tgttccatcc tgtattccga tctcggtgtc
       61 ggaacatact ggagcccaac cgacgacact ggcggatcgt tgccgtgatc tctgggagga
      121 ataagtgagc gactcagcag acgatgatgc ggcgggcgac gacctgcggc ggtatgcctt
      181 ccatatggat ccgcaggtga cattgaccgg ggatacgtgg accgcgtcgt atcccggtgc
      241 ggactggtcg gtttcgggtc ccagcagcca ggaggcgttg cgacggctgg gcgaggaatt
      301 cgtccggcga cagaacgacg gcgacgaccc acttgcctat gctgatcagc tatatcgtcg
      361 gcacctgcgt gaaccggtgc ccggtgtcta tgccgtcgac aacgagctgt atcgcgaact
      421 cgtacatgcg ccctcagccg aacggcagcg cacagtcaaa gaggctgagc gcagacggcg
      481 gttgggccag tcgtacacgc tgttggacta tcagcgtgag cagggaacct ctcctacctg
      541 atcagctctg gggcacttcg atgtcggaga acagcacgcg cacaccaccc gtcgacaatc
      601 gcgccatcgc ctcgaagaac gcgccggcct ccggtgtggt caccgcgtcg ctggcagcgg
      661 agtagtccgg gtagtacagg tcgatcgagc gatatgcggg cgtcgggctg ccgtcttcct
      721 tcggccacac cttggacgtt tcgaaccgga tgtgtcccgg tatccggcga gcggcctcga
      781 gctgctccga ctcgtatgcg gcctcgaagg cgtccgggtc gatgggattg tcgtagatga
      841 ccgtgatctt cgtctcggac aatggatttg cctcgcatcg ctgtcggcgg attgaaccag
      901 tggcgtggca agtctacgag gtacacgcag gcaccagttc cgtttcagcc gttggggcag
      961 ctggtcgtcg gaatgaagag cgaccagcac catcccggtg gaagcggtgg agtcaggtta
     1021 ggcggaggtg gcggtggcgc cggcggggcc tctccctcga agatgttcga ggcgacgttg
     1081 ccctgcccgt ggtacacgta ccagtacgtg tggcacgcgt tgttgtccca caccaccggg
     1141 ttcacccgaa ggttccccgt ctgcaccggc gggtcccccg ggcaccagtt gcacggacct
     1201 gatgggttgt cctccggaca gccgggccac gcgccgagag gaaccggagc gcccggcagg
     1261 gcctgggcgg tgccgacgcc cagcccgatc ccgacgagcg caacgccggc cgacaacagt
     1321 gctccggcga tggtcttcct tcgcttgccc acggtgtgtt cctttgcgct cgcgccgcct
     1381 gatgcggtgt tgccagcagt acacagaaac cggatcgcta ccgatcccaa agatcatgcg
     1441 gagccgggct cgtccacact ggtcacgact cccccgctgt tcccttcgtg cggcgaccgc
     1501 gctgaggctc aaccgccggc ggctgcgtcg atcgcccgac ggatccgctg ttcgctcacc
     1561 ggatgcgggg tgccgagctg ctgggcccac aggcttaccc gcaactcctc gatctgccag
     1621 gcgatgtcgc gcacgtcggc ggcttgctgc cggcccgacg gcaacgcgcg aa
//
LOCUS       DASYLX010000873         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1589038,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000873 DASYLX010000000
VERSION     DASYLX010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589038"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="lexA"
                     /locus_tag="VGP27_20375"
     CDS             complement(<1..594)
                     /gene="lexA"
                     /locus_tag="VGP27_20375"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV7853690.1"
                     /translation="MSEDRTDTQNSTTGPGNAGERRAPQSALTERQRTILQVIRASVT
                     SRGYPPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRAVDVRGSDDMVTPI
                     VGTDVAGSDALPEPTFVPVLGRIAAGGPILAEQAVEDVFPLPRELVGEGSLFLLKVVG
                     ESMIDAAICDGDWVVVRQQNVADNGDIVAAMIDGEATV"
     gene            805..1275
                     /locus_tag="VGP27_20380"
     CDS             805..1275
                     /locus_tag="VGP27_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV7853691.1"
                     /translation="MTVLDTREVLAGPVVRGPHGPTAARRPYRAPQSRRRPGGAPMRY
                     RGPGVVMSRASHRRRPITPATTVGLALLAALITAWLGVVAQFGGVVGTPSSSAPVPDT
                     LAVVQVQAGETLQQVAHRVAPAAPVAHVVDRIRELNQLDSASLDAGQTLIAPVG"
     gene            1399..1857
                     /gene="nrdR"
                     /locus_tag="VGP27_20385"
     CDS             1399..1857
                     /gene="nrdR"
                     /locus_tag="VGP27_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV7853692.1"
                     /translation="MHCPFCRHPDSRVVDSRETDEGQAIRRRRSCPECGRRFTTVETA
                     VLAVVKRSGVTEPFSREKVIKGVRRACQGRQVDDDALNLLAQQVEDTVRAAGSPEVPS
                     HEVGLAILGPLRDLDEVAYLRFASVYRSFSSADDFEREIEALRAHRTVSS"
     gene            complement(1871..>2020)
                     /locus_tag="VGP27_20390"
     CDS             complement(1871..>2020)
                     /locus_tag="VGP27_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEV7853693.1"
                     /translation="AAAVRITDYLSRDLTIVQGKGSVIRTQWSADGWVRVAGRVVDDG
                     TRQID"
BASE COUNT          304 a          689 c          689 g          338 t
ORIGIN      
        1 cacggtggcc tcgccgtcga tcatcgccgc gacgatgtcg ccgttgtcgg cgacgttctg
       61 ctgacgcacc acgacccagt cgccatcgca gatcgccgcg tcgatcatcg actcgccgac
      121 caccttgagc aggaacagcg agccctcgcc gacaagctcg cggggtagcg ggaagacgtc
      181 ttcgacggct tgttcggcca gtatcggccc gcctgccgcg atgcggccca gcaccggcac
      241 gaaggtcggc tcgggtagcg cgtccgagcc ggcgacatcg gtgcccacaa tcggcgtcac
      301 catgtcgtcg gagccgcgca cgtcgaccgc gcgcggccgg ttcggatcgc gccgcaggta
      361 gcccttccgc tccagggtcc gcaactggtg agcaaccgac gaggtcgacg tcagacccac
      421 cgcatcgccg atctccctga tgctcggtgg atagccgcgg ctggtcaccg aggcgcgaat
      481 gacctgcagg atcgtgcgct gccgctcggt cagtgcgctc tgcggcgcgc ggcgttcgcc
      541 tgcgttgccg ggccctgtag tgctgttctg agtgtcggtg cggtcctcgc tcatggcgct
      601 gaatgtagtc gccggacgcc cgataatcaa acatgtgttc gaggcgtgtc gcaatctgtg
      661 gatccgcgac ctgcggccga ccggttttgt cggtggcctg ccctatcgtt tcgcaacagt
      721 tcgatcacat gttctattaa tcgaacacct gatcgattag agttcgaaca taagagcgaa
      781 cgatcgaagc ggaaggcgag gcagatgaca gttctggaca cgagagaagt cctggcgggt
      841 cccgtcgtgc gggggcctca cggcccgacg gcggcgcggc ggccataccg cgcacctcag
      901 agccggcgac ggcccggcgg tgcgccgatg cgctaccgcg gtcccggtgt cgtgatgtcc
      961 cgcgcatcgc atcgcaggcg cccgatcacg cccgccacca ccgtcgggct ggcgctgctc
     1021 gccgcgctga tcacggcctg gctcggtgtg gtggctcagt tcggcggtgt cgtcggcacc
     1081 cccagttcgt cggcgccggt gcccgacacg ctggccgtgg tccaggtaca ggcgggggag
     1141 acgctgcagc aggtggcgca ccgggtggca cccgccgcgc ccgtcgccca tgtcgtcgac
     1201 cggatccggg aactcaacca actggactcc gcgtcgctcg acgccgggca gacgctgatc
     1261 gcaccggtcg gctgagccat gcgcggtgaa acgcgctgcc gcgcaacggc cagtcggtct
     1321 gcgggttttg ggcgggatac gaaggtacgc tcggaggcgt tcgagttggt tgtccgtcgg
     1381 cacggcgaag gagcagtcat gcactgtccg ttctgccgtc atcccgactc ccgtgtcgtc
     1441 gactcccggg aaaccgacga aggtcaagcg atccggcgcc gtcgctcctg cccggagtgc
     1501 ggccgacggt tcaccaccgt cgagaccgca gtactggccg tcgtcaagcg cagcggggtc
     1561 accgagccgt tcagccgcga gaaggtcatc aagggggtgc ggcgcgcctg ccagggtcga
     1621 caggtcgacg acgatgcgct gaacctgttg gcacagcagg tcgaggacac ggtgcgcgcc
     1681 gccgggtcgc ccgaagtgcc cagccacgag gtcgggttgg ccatcctggg gccattacgg
     1741 gatctcgacg aggtcgccta cctgcgcttc gcgtccgtat accggtcgtt ctcctcggcg
     1801 gacgacttcg agcgcgagat cgaggcgctt cgggcgcacc gtacggtctc gagctagggc
     1861 ccaccgttgc tcagtcgatc tgcctggttc cgtcgtccac cacgcggcct gcgacccgaa
     1921 cccacccgtc ggcgctccac tgcgtccgga tgaccgagcc cttgccctgc acgatggtga
     1981 ggtcgcggct gaggtagtcg gtgatgcgaa ccgccgccgc
//
LOCUS       DASYLX010000874         8978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1697374,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000874 DASYLX010000000
VERSION     DASYLX010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697374"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="VGP27_20395"
     CDS             complement(<1..1707)
                     /locus_tag="VGP27_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7853694.1"
                     /translation="MVALSDRLDFVIGRTNAKKLDEHFGIRTVNDLLRHYPRKYNEGM
                     TVRGEGEELDLEEGEHVTFVDTITEAKSGDMKPVWDKKRKKMRYPKWLRVTLGEHRPK
                     VTATFFNANWMIENLPAGTRIMLSGEVKYFKGTMALSHPAYLVLQSPTGKEIGTKSLK
                     TIATTSGATGDELLSVFEREFFPIYAASSKVQSWDIYACVRQVLDVLDPVADPLPESL
                     LRQADLIPEDEALRAIHLAETAAERERARARLTYDEALGLQWALVERRNSELGESGPP
                     APETDDGLLAALMQQLPFELTRGQHEVLATLSAELAGTRPMNRMLQGEVGSGKTIVSV
                     LAMLQMIDAGYQCALLAPTEVLAAQHARSVRDVLGPLAMAGQLGGCDSATRVALLTGS
                     MSAQQKKQVRDEVAAGEAGIVIGTHALLQDAVDFHNLGMVVVDEQHRFGVEQRDRLRA
                     KAPQGLTPHLLVMTATPIPRTVALTVYGDLETSTLRELPRGRQPIATNTIFIKEKPQW
                     LDRAWQRTIEEVRAGRQAYVVASRIDENDKKSNEKEDGPPATTVVELLERLRQGPLSG
                     LRL"
     gene            complement(1710..3362)
                     /locus_tag="VGP27_20400"
     CDS             complement(1710..3362)
                     /locus_tag="VGP27_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV7853695.1"
                     /translation="MAGSGRRLDASALRDWAHTSVGDLISHTDEINRLNVFPVADADT
                     GTNMLFTMRAAWAQADSSTAGGDVATVAAALATGALHGARGNSGVILSQILRGFADVA
                     AEAAHELGDVLADVDGRLLAASLRHALGLVVASMGKSVPGTIVSVLKAAAEAAEDAVA
                     DDGPLGEVVTAAADAAAIALERTTEQLDVLTDAGVVDAGGRGLLVLLDALSKTVSGHA
                     PVRQEYVPAPPQVDTTVAAPAPRFEVMYLLSGCRPDGVEHLRQRLDELGDSVAIAASA
                     ADGGGRYSVHVHSDDAGGAIEAALAFGTPSRIQITALTGGSGVRAPGGWARERAVLAV
                     VDGDGAAELFAGEGADILCPDPDANPTSAVTAKQLLRGLVDAGAAQIMVLPNGFVAAE
                     ELVAGCTAAIGWGIDVVPVPAGSMVQGLAALAVHDADRQAVDDGYTMARAAAGARHGS
                     VRLATEEALTWAGTCRPGDGLGIAGDEVLIVAEDITAAGAGLIDLLLVAGGELVTVLS
                     GVGVDPAVGEALKDHVHHEHLGTELVTYHTGHRGDALLIGVE"
     gene            3591..3785
                     /gene="rpmB"
                     /locus_tag="VGP27_20405"
     CDS             3591..3785
                     /gene="rpmB"
                     /locus_tag="VGP27_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV7853696.1"
                     /translation="MAAVCDICGKSPGFGKSVSHSHRRTSRRWDPNIQRVRAVTRPGG
                     NKKRINVCTSCLKAGKVTRG"
     gene            complement(3855..4538)
                     /locus_tag="VGP27_20410"
     CDS             complement(3855..4538)
                     /locus_tag="VGP27_20410"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV7853697.1"
                     /translation="MTARPLADQVDAGWARALEPVGDQVAQMGEFLRSEIAAGQRYLP
                     AGQNVLRAFTFPFDQVRVLIVGQDPYPTPGHAVGLSFSVASDVRPLPRSLENIYKEYS
                     SDLGYPAPANGDLSPWAERGVMLLNRVLTVRPGTPASHRGKGWEAVTECAIRALVARA
                     QPLVAVLWGRDASTLKPMLDGGDCVAIESPHPSPLSASRGFFGSRPFSRANELLEKMG
                     AEPIDWRLP"
     gene            complement(4563..5522)
                     /locus_tag="VGP27_20415"
     CDS             complement(4563..5522)
                     /locus_tag="VGP27_20415"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV7853698.1"
                     /translation="MANEERADSLAGLGEFAVIDRLVADRDQPAVVAVGPGDDAAVVT
                     AADGRTVVSTDMLVEGRHFRLDWSSPHDVGRKAIAQNAADIEAMGATATAFVVAFGAP
                     GDTSAAQTLELADGMWHEARKFGAGVVGGDLVSAPQWVISVTVLGDLAGRAPVLRSGA
                     RVGDILAVVGGLGRSAAGYDMWRNDIDGFDTLRRLHVAPEPPYGQGRVAADGGATAMT
                     DVSDGLAADLGHIADASGVGINVSTAALTADRDALADAAAAVDVDAWDWVLGGGEDHG
                     LVATFTGTPPQGWRAIGAVIDGPPRVLVDGDAWHGNPGWQSYR"
     gene            5698..5940
                     /locus_tag="VGP27_20420"
     CDS             5698..5940
                     /locus_tag="VGP27_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEV7853699.1"
                     /translation="MVEAFMLIQTDVGRAEVIAKQLAELTGVRCAEYVTGPYDVVVRV
                     EGDTIDDLQNRVVPSVQQITGITRTVTCPIAGGARP"
     gene            5951..6499
                     /locus_tag="VGP27_20425"
     CDS             5951..6499
                     /locus_tag="VGP27_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HEV7853700.1"
                     /translation="MAQTTDDGADGPPRSVLIAAIAVAVAAVVAVLAIAVLRQSPAQH
                     EPVPIATVPAPKAASRECTALTAALPEQLGDYRRAPAADPAPPGAAAWRAESDPDPVI
                     LRCGLDRPAEFVVGSPLQEVEPVQWFKLTDAGRSTWFAVDRPVYIALTLPSGSGPTPI
                     QEISEAISQTMPAKPLDPAPAR"
     gene            complement(6496..7626)
                     /locus_tag="VGP27_20430"
     CDS             complement(6496..7626)
                     /locus_tag="VGP27_20430"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEV7853701.1"
                     /translation="MTARQRPGSAGRVRVAVVYGGRSSEHAISCVSAGSILRHLDPER
                     FEVVAVGITPEGSWVLTNADPDTLAIADGRLPEVSGASGTALALAADPSRGGQLLSLR
                     QGAAGQLLASVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKK
                     LLVADGLPVGDHVVLRPDRETLDLEERERLGLPVFVKPARGGSSIGVSRVAAWDQLPA
                     AIADARIHDPKVIVEAAVSGRELECGVLELPDGRVEASTVGEIRVVGVRGREDGFYDF
                     ATKYLEDAAELDVPAKVEDKNADEVRRLAIRAFRAIDCQGLARVDFFLTDDGPVVNEI
                     NTMPGFTTISMYPRMWAASGVDYPALLAAMVDTALARGTGLR"
     gene            complement(7732..8841)
                     /locus_tag="VGP27_20435"
     CDS             complement(7732..8841)
                     /locus_tag="VGP27_20435"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEV7853702.1"
                     /translation="MGGVYGDSTRSVKASGAEAVPGSPIMPPPVPAAAYHIPASEDDS
                     LDSYGRYHNPTWRHLESALAELEGATAALTFGSGMAAITSALRALTRPGATLVVPADG
                     YYQVRRYAVEYIAPQGIRVIEAKCADMVDAADGADVVLAETPSNPGLDVVDLHRLAMT
                     CRQRGATLIVDNTTATPLGQQPLSLGADLVVASATKALSGHHDLLAGYVAGSRVEQMA
                     AIEHERRLAGPILGVFEAWLVLRSIGTAGLRFERQCQNALAIALALRAHPGVRFLRYP
                     GLPDDPSHEIANRQMKRFGGIVAIELESAAAVHAMLRNSNLLVSSTSFGGIHSSVDRR
                     ARWGDPVGEGFARISAGIEDTDDLVADIEQALAHP"
     gene            complement(8846..>8978)
                     /locus_tag="VGP27_20440"
     CDS             complement(8846..>8978)
                     /locus_tag="VGP27_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEV7853703.1"
                     /translation="VLALAQSYDVEMPLTDAVNRVCHKGLSVPEAVALLLGRSTKPE"
BASE COUNT         1394 a         3218 c         2932 g         1434 t
ORIGIN      
        1 gagccgcagt cccgacagcg gtccttgccg aagacgctcg agcaactcca ccaccgtggt
       61 cgcaggcggc ccgtcctctt tctcgttgct tttcttgtcg ttctcgtcga tgcgcgatgc
      121 gacgacgtag gcctgacgac ccgcccggac ctcctcgatg gtccgctgcc atgcccggtc
      181 gagccattgc ggcttctcct tgatgaagat cgtattagtg gcgatcggtt gccggccgcg
      241 cggcagttcg cgcaacgtcg aggtctccag gtcgccgtag accgtgagcg ccacggtgcg
      301 cgggatcggg gtggcggtca tcaccagcag gtgcggcgtc agaccctgcg gcgccttggc
      361 gcgcagtcga tctcgctgct cgacgccgaa gcggtgctgc tcgtccacca ccaccatgcc
      421 gaggttgtga aaatcgacgg catcttggag cagtgcgtgg gtgccgatga cgatccccgc
      481 ctcgcctgcg gcgacctcgt cacgcacctg cttcttctgc tgcgctgaca tcgatccggt
      541 cagcagcgca acccttgtcg cgctgtcgca gccgccgagc tggccggcca tcgccaacgg
      601 gccaaggaca tcgcgcaccg agcgcgcatg ctgggccgca aggacttccg tgggagccag
      661 cagtgcacat tgatagcccg cgtcgatcat ctgcagcatg gccagcaccg agacgatggt
      721 cttcccggag ccgacctcgc cctgcagcat ccggttcatc ggacgggttc cggcgagttc
      781 ggccgaaagc gttgcgagca cctcgtgctg gccgcgggtc agttcgaacg gcagctgttg
      841 catcaacgcc gcgagcagcc cgtcgtccgt ctccggtgca ggcggccccg attcgcccaa
      901 ttcactgttt cggcgctcca ccaacgccca ttgcagcccc agcgcctcgt cgtacgtcag
      961 gcgggctctg gcacgttcgc gttcggccgc cgtttcggcg agatggatcg cacgcagtgc
     1021 ctcgtcctcc ggaatcagat cggcttggcg cagcagtgac tcgggtagcg gatcggcgac
     1081 gggatccagc acgtcgagca cctgccgcac gcaggcgtag atatcccagc tctgcacctt
     1141 ggaactggcg gcatagatcg ggaagaactc gcgctcgaac accgaaagga gttcgtcgcc
     1201 cgtcgcaccc gatgtggtgg cgatcgtctt gagcgacttc gttccgatct ccttgccggt
     1261 cggcgactgc aacacgagat aggccggatg gctgagcgcc atggtgccct tgaagtactt
     1321 cacctcgccg gacaacatga tgcgggtgcc tgcgggcaga ttctcgatca tccagttggc
     1381 gttgaagaac gtcgcggtga ccttgggacg gtgctcgccg agcgtgacac gcagccactt
     1441 ggggtagcgc atcttctttc tcttcttgtc ccagacgggt ttcatgtcac cggatttcgc
     1501 ttcggtgatg gtgtccacga acgtcacgtg ttcgccctct tcgagatcga gctcctcccc
     1561 ctcgccgcgg acggtcatgc cctcgttgta cttgcgcggg tagtgccgca gaaggtcgtt
     1621 cacggtgcgg ataccgaagt gctcgtcgag cttcttcgcg ttcgtcctgc cgatgacgaa
     1681 atccagccgg tcagacagtg cgaccacggc tactcgaccc cgatcaacaa cgcgtcgccc
     1741 cgatgaccgg tgtggtaggt caccagctcg gtgcccagat gttcatggtg cacgtggtcc
     1801 ttgagtgctt cgccgacagc gggatccacg ccgacgccgc tcagcacggt gaccagctca
     1861 ccgcccgcga cgaggagcag atcgatgagg cccgcgcctg ctgcggtgat gtcctcggcg
     1921 acgatcagca cctcgtcgcc cgcgatgccg agcccgtcgc cggggcggca ggtgcctgcc
     1981 cacgtcagcg cctcctcggt ggcgagccga accgaaccgt gccgcgcgcc cgcggcggcg
     2041 cgcgccatcg tgtaaccgtc gtcgacggcc tgccggtcgg cgtcgtgcac ggcaagcgcg
     2101 gccaggccct gcaccatcga acccgcgggc accggaacca cgtcgatgcc ccacccgatc
     2161 gccgccgtgc agcccgcgac caactcctcg gcggcgacga aaccgttggg caggaccatg
     2221 atctgggcgg cacccgcgtc gacgaggccg cgcagcagct gtttggcggt cactgccgac
     2281 gtggggttcg cgtcgggatc gggacacagg atgtcggcgc cttcgccggc gaagagctcg
     2341 gccgcgccgt cgccgtcgac gaccgcgagc accgcacgct cacgcgccca gccgcccggc
     2401 gcgcgcactc cggagccacc ggtgagcgcc gtgatctgaa tgcggctggg tgtgccgaac
     2461 gccagcgcgg cctcgatggc gccgcccgcg tcatcggagt gcacgtgcac cgaatagcga
     2521 ccgccgccgt cagccgcgga cgcggcgatg gcgaccgaat cgccgagttc gtcgagccgc
     2581 tgacgaaggt gttcgacacc gtcgggcctg catccgctca gcaggtacat cacctcgaaa
     2641 cgtggagcgg gtgccgcgac ggtggtgtcg acttggggcg gggccggcac atactcctgt
     2701 cggaccggtg catgaccgga aacggtcttc gacagcgcat cgagcaacac cagcagtcca
     2761 cgaccgcccg cgtcgacgac tccggcgtcg gtcaacacgt ccagttgctc ggtggtccgc
     2821 tcgagtgcga tcgccgccgc atcggccgcc gccgtcacca cctcgccgag ggggccgtca
     2881 tcggcgacgg cgtcttcggc cgcttccgcg gcggccttca gcaccgacac gatggttccc
     2941 ggcaccgatt tgcccatcga ggccacgacc aggccgaggg cgtgccgaag cgatgcggcg
     3001 agcaggcggc cgtcgacgtc ggccagtaca tcgcccagct cgtgggcggc ttcggcggcg
     3061 acgtcggcga agccgcgcag aatctgcgaa aggatcaccc cggagttgcc cctggcgccg
     3121 tgcagggcgc cggtggccag ggccgcggcg accgtcgcca cgtctccgcc ggccgtcgag
     3181 gaatccgcct gcgcccacgc cgcccgcatg gtgaacagca tgttcgtccc ggtgtcggcg
     3241 tcggcaacgg ggaagacgtt cagccggttg atctcgtcgg tgtgggagat caggtcgccc
     3301 accgaggtgt gcgcccagtc gcgcagggct gacgcgtcca gccgccgacc agacccagcc
     3361 atcgatcacc caccttccac cgacgaccct tccaccgacg acaatggacc ccccggtggg
     3421 cgccagccta tccattgcgc cggacatcgc cggtcacatc cacccgcctg gtcggcaccc
     3481 ggtggacggc gttttggcgt tctctttgtg ggccggtatc ctggtcaggt tgtcgggtca
     3541 cccgcggttg cggtcgctgg cccccagtac tgattcaagg agttctcgat atggctgccg
     3601 tctgcgatat ctgcgggaag agccccggct tcggtaagtc ggtctctcac tcccatcgcc
     3661 ggaccagccg tcggtgggac cccaacatcc agcgcgtgcg cgccgttacc cgtcccggcg
     3721 gcaacaagaa gcgcatcaac gtctgcacgt cctgcctcaa ggcgggcaag gtcacccgcg
     3781 gctaactccc gcccaaaatc acgtttcggc gattttgtgc gagtagaacg cgccaatatg
     3841 tcgatctcgg cgcgttaggg cagccgccag tcgatcggct cggcgcccat cttctcgaga
     3901 agttcgttgg cgcggctgaa cggccgcgac ccgaagaatc cccgcgacgc cgacagcggc
     3961 gagggatgcg gcgactcgat cgccacgcag tcaccgccgt cgagcatcgg cttgagcgtg
     4021 gacgcgtcgc gcccccacaa caccgccacc aacggctgcg cccgcgccac caacgcccgg
     4081 atcgcgcatt cggtgaccgc ctcccaaccc ttaccgcgat gcgaagccgg cgtgccggga
     4141 cgcaccgtca gcaccctgtt gagcagcatc acgccgcgtt cggcccacgg actgagatcg
     4201 ccattggccg gggcgggata gcccaggtcg ctgctgtact ccttgtagat gttctccagg
     4261 ctgcgcggta gcggacgcac gtcggacgcg accgagaagc tgagcccgac cgcatgcccg
     4321 ggcgtcggat aagggtcctg cccgacgatc agcactcgca cttgatcgaa cggaaacgtg
     4381 aaggcgcgca acacattctg accagcgggc agataccgct ggcccgcggc gatctcgctc
     4441 ctgaggaact cccccatctg ggcgacctga tcgccgacgg gttccagcgc gcgcgcccag
     4501 cccgcatcga cctggtcagc gagggggcgt gcggtcacga caagtcaacc tagcggctcg
     4561 gttcaccgat atgactgcca gcccggattt ccgtgccacg cgtcgccgtc gaccagcacc
     4621 cgcggcggac cgtcgatcac tgcgccgatg gcccgccagc cctgcggcgg cgtgccggtg
     4681 aacgtcgcga ccaggccatg atcctctccg ccgccgagca cccagtccca tgcgtcgacg
     4741 tccaccgcgg cggcggcgtc ggcgagggcg tcgcggtcgg ccgtcagcgc cgcggtggac
     4801 acattgatgc ccactccgga ggcatcggcg atatggccca ggtcggccgc cagcccgtcg
     4861 gagacgtccg tcatcgccgt cgcaccgccg tcggccgcga cgcggccctg tccgtaaggc
     4921 ggctccggcg cgacatgcag ccgccgcagg gtgtcgaagc cgtcgatatc gttgcgccac
     4981 atgtcatatc cggcggcaga ccggcccaat ccgccgacga cggccaggat gtcacccacc
     5041 cgcgccccgg aacgaagcac cggcgcgcgc ccggcgagat cgccgagcac cgtcaccgag
     5101 atcacccact gcggggcact caccagatct ccaccgacga cccccgcgcc gaacttgcgt
     5161 gcctcgtgcc acatgccgtc tgcgagctcg agcgtctgcg ccgccgaagt gtctcccggt
     5221 gcgccgaacg caacgacgaa tgcggttgcc gttgcgccca tcgcctcgat gtcggcggcg
     5281 ttctgtgcga tggcctttcg gcccacgtcg tgcggcgacg accagtcaag gcggaagtgc
     5341 ctgccctcca ccagcatgtc ggtggacacc accgtcctgc cgtcggccgc cgtcaccacc
     5401 gcggcgtcgt cacccggacc gacggcgacc accgcgggct ggtcccggtc ggccaccaac
     5461 cggtcgatga cggcgaactc ccccagccca gccaggctgt cggcccgctc ctcgttggcc
     5521 atggcacctc cggttccgct cataactcga ctccggccct gcaagcgtgg gcccaccggt
     5581 cctcgctgca ctgcgatcct ctcgcgctcg tcttcgtgcg ctgaacccct ggcgatcctg
     5641 cggtactttt gggccctgcg gcgtggagtc tatgcagcgg aacggaggag acaccgggtg
     5701 gtcgaggctt tcatgctgat ccagaccgac gtgggccgcg ccgaggtcat cgccaagcag
     5761 ttggccgagt tgaccggtgt gcgctgcgcc gagtacgtca ccgggcccta tgacgtcgtg
     5821 gtgcgcgtcg agggcgacac cattgacgac ctgcagaaca gggtcgtccc gagcgttcag
     5881 cagatcacgg gcatcacgcg caccgtgacc tgccccatcg ccggtggagc acggccctag
     5941 agttgcccat gtggcacaga cgaccgacga cggtgcagac ggtccgccgc ggtctgtgct
     6001 gatcgccgcg atcgcggtgg ccgtcgcggc ggtggtcgcg gtgctcgcga tcgcggttct
     6061 gcggcagtcg ccggcacagc acgagccggt tcccatcgca acggtccccg ccccgaaggc
     6121 ggccagccgc gagtgcactg cgctgacggc cgcgctgccc gagcagttgg gcgattaccg
     6181 gcgagcaccg gcggccgacc ccgcaccgcc gggcgcggcc gcttggcggg cggaatcgga
     6241 tcccgacccg gtcatcctgc gctgcgggct ggaccgtccc gcggagttcg tggtcggctc
     6301 gcccctgcag gaagtcgaac ccgtgcagtg gttcaagctg accgacgcgg gcaggagcac
     6361 ttggttcgcc gtggatcgcc cggtatatat agcccttacg ctgccgagcg gatcggggcc
     6421 gacccccatc caggagatct ccgaggcgat atcgcagacg atgcccgcca aaccgctcga
     6481 cccggcgcct gcccgctagc gcaggccggt gccccgcgcc agcgccgtgt ccaccatcgc
     6541 cgccaacagg gccggatagt ccacaccgct cgccgcccac atcctcggat acatcgagat
     6601 cgtcgtgaag ccgggcatgg tgttgatctc gttgacgacc gggccgtcgt cggtcaggaa
     6661 gaagtcgacc cgggccaggc cctggcagtc gatcgcgcga aacgcgcgga tggccagtcg
     6721 gcgcacctcg tcggcgttct tgtcctcgac ctttgcgggc acgtcaagtt cggcggcgtc
     6781 ctcgagatat ttggtggcga agtcgtagaa cccgtcctcg cggccgcgca cgcctaccac
     6841 ccggatctcg ccgacggtgc tcgcctcgac cctgccgtca gggagttcca ggacgccgca
     6901 ttcgagttcg cgccccgata cggcggcctc gacgatcacc ttggggtcgt gtatccgggc
     6961 atcggcgatg gcggcaggca gctgatccca cgccgcgaca cggctgaccc cgatcgacga
     7021 tccgcctcgc gccggcttga cgaacaccgg caacccgagc cgctcgcgct cctcgaggtc
     7081 cagagtttcc cggtccggtc gcagcactac atggtcgccg accggcaggc cgtccgcgac
     7141 cagcagcttc ttggtgaact ccttgtccat gcccaccgca ctggccagca cgccggcgcc
     7201 gacgtagggc accccggcca gctcgagcag tccctggatc gtgccgtcct cgccgtacgg
     7261 tccgtgcagc accgggaaga ccacgtcgac ggaggccagc agctgccccg ccgcgccctg
     7321 gcgcagagac aggagctgcc caccgcgact cgggtcggcc gccagcgcca gggcggtacc
     7381 cgatgcaccg ctgacctcgg gcagccgtcc gtcggcgatc gccaaggtgt ccgggtcggc
     7441 gttggtcagc acccacgagc cctcaggtgt gatgccgacc gcgaccactt cgaagcgctc
     7501 tggatcgagg tggcggagga tgctgcctgc ggaaacgcac gagatcgcgt gctcggagct
     7561 gcgtccgccg tagacgacgg cgacacggac gcgaccggcg gagccgggcc gttggcgggc
     7621 agtcacaacc tagagaggct accgtccccg ccgaaagccg cacggtctac cgctgccggc
     7681 tgggcttttc tacgatcgtc gagcgcccgt cctcatccga gcgcttgatc gtcacgggtg
     7741 cgccagcgcc tgctcgatgt cggcgaccag gtcgtcggtg tcctcgatgc ccgccgagat
     7801 ccgggcgaag ccctcgccga ccggatcgcc ccagcgggcc cgccgatcca ccgagctgtg
     7861 gataccgccg aagctggtcg acgacacgag cagattgctg tttcgcagca tcgcgtgtac
     7921 ggcggcggcg ctctcgagct cgatcgccac gatcccgccg aagcgcttca tctgccgatt
     7981 ggcgatctcg tgcgacgggt cgtcgggcag tcccgggtag cggaggaacc gcacgccggg
     8041 atgtgcccgc aacgccaacg cgatcgcgag cgcgttctgg cactgccgct cgaaccgcag
     8101 acccgcggtt ccgatgctgc gcagcaccag ccaggcctcg aagacgccca ggatggggcc
     8161 tgcgagccga cgctcgtgct cgatcgcggc catctgctcg acgcggctgc ccgcgacgta
     8221 tcccgcgagc aggtcatggt gacccgacaa cgccttcgtg gcgctggcca ccaccaggtc
     8281 ggcaccgagc gacagcggtt gttggccaag gggtgtcgcg gtcgtgttgt cgacgatgag
     8341 ggtcgcaccg cgttggcggc atgtcatcgc gaggcgatgc agatccacca cgtcgaggcc
     8401 ggggttcgac ggtgtctcgg cgagcaccac gtcggcgccg tccgccgcgt cgaccatgtc
     8461 ggcgcatttc gcctcgatca ccctgatgcc ctgcggcgca atgtattcga ccgcatatcg
     8521 ccgcacctgg tagtaaccgt cggccggaac aaccagcgtg gctccaggtc tggtcaatgc
     8581 gcgaagcgcc gacgtaatcg ccgccatgcc ggaaccgaat gtcaaagccg ctgtcgcgcc
     8641 ttccaattcg gccagagccg actcgaggtg gcgccaggtc ggattgtgat agcgcccgta
     8701 gctgtcgagc gagtcgtctt cactcgctgg gatgtggtag gcggccgcag ggacgggcgg
     8761 cggcatgatg ggtgagcccg gaaccgcttc tgcaccactg gctttgacgc tccttgtgga
     8821 gtctccgtat accccgccca taccgctact ccggcttggt gctgcggccg agcagcagcg
     8881 cgaccgcctc cggaacggat aatcccttgt ggcagacccg gttgaccgca tcggtcaacg
     8941 gcatctcgac gtcgtagctt tgggccagtg ccagcacc
//
LOCUS       DASYLX010000875         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_600521,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000875 DASYLX010000000
VERSION     DASYLX010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600521"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGP27_20445"
     CDS             complement(<1..834)
                     /locus_tag="VGP27_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7853704.1"
                     /translation="MRGRVARSAVALGSAGLVVLSMAACQQSKPEEGTSQTDLKIVEQ
                     VQIDENGGEVKATAGAAAADPAGDGKANCPPVSIAMAGALNGPDAALGINIKNGVQLA
                     IDKHNAANPGCQIQLKPFDTEGDPQKATAIAPQIVDDQYTIGLVGPAFSGETKATGGV
                     FNQAGLVAVTASATNPTLSENGWKTFFRGLANDAVQGPSVANYMKNGLSYKKVCVIDD
                     STDYGLGLATAVRDTLGPLADSGCNISVKKGDKDFSAAVTQVKGVSPDAVFYGGYYSE
                     AA"
     gene            complement(957..1580)
                     /locus_tag="VGP27_20450"
     CDS             complement(957..1580)
                     /locus_tag="VGP27_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HEV7853705.1"
                     /translation="MTGTTTDDAKPHRVLIAEDEALIRLDLAEMLREEGYEVVGEAGD
                     GQEAVELAEKLRPDLVIMDVKMPRRDGIDAASEIADKRIAPIVVLTAFSQRELVERAR
                     DAGAMAYLVKPFSISDLIPAIELAVSRFGEISALEQEVATLSDRLETRKLVERAKGLL
                     QAKHQMSEPEAFKWIQRAAMDRRTTMRRVAEVVLETLDSPEDAAPPS"
     gene            1648..1721
                     /locus_tag="VGP27_20455"
     tRNA            1648..1721
                     /locus_tag="VGP27_20455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1682..1684,aa:Leu,seq:caa)
BASE COUNT          298 a          635 c          603 g          377 t
ORIGIN      
        1 agccgcctcc gagtagtagc cgccgtagaa cacggcgtcc gggctcactc ccttgacctg
       61 agtgaccgcg gcggagaagt ccttgtcgcc cttcttcacc gagatgttgc atccggagtc
      121 ggcgagcggg cccagcgtgt cccgcacggc cgtcgccagg ccgaggccgt agtcggtgct
      181 gtcgtcgatc acacagacct ttttgtaact cagcccgttc ttcatatagt tcgcgaccga
      241 cgggccctga acggcgtcgt tcgccagacc gcggaagaac gtcttccagc cgttctcgct
      301 gagggtcggg ttcgtcgccg aggcagtgac ggccaccagg cccgcctggt tgaacacgcc
      361 accggtggct ttcgtctcgc ccgagaacgc cgggccgacg aggccgatgg tgtactgatc
      421 gtcgacgatc tgcggtgcga tcgccgtggc cttctgcggg tcgccctcgg tgtcgaaggg
      481 cttcagctga atctggcaac cggggttggc ggcgttgtgc ttgtcgatgg ccagctgcac
      541 gccgttcttg atgttgatgc cgagtgccgc atcagggccg ttgagcgcac ccgccatggc
      601 gatcgacacc ggcgggcagt tcgccttacc gtcgccggcc gggtcggcgg ccgcagcgcc
      661 cgccgtggcc ttgacctctc cgccgttctc gtcgatctgt acctgctcga cgatcttcag
      721 atctgtctgt gatgtcccct cttcgggctt ggactgctga caggcagcca tgctgagcac
      781 cacaagtcct gcgctaccga gcgctactgc gctccgtgcc acgcgaccgc gcacgtttca
      841 cctccgactc gatgttttca tcggcatcga cgatccggct ctcctgagcc agggcgctga
      901 cgcttcggaa agaacatagc caacacatgg ccgtagtgcg cgttttccgg gtattgctag
      961 ctcggcggcg cggcgtcttc gggcgagtcc agtgtctcca gcacgacttc ggcgacccgc
     1021 ctcatcgtcg tccggcggtc catcgcggcc cgttgaatcc acttgaacgc ctcgggttcg
     1081 gacatctgat gcttggcctg gagcaaaccc ttcgcccgtt caacgagttt gcgcgtctcc
     1141 agtcgatccg acagcgtcgc aacttcctgc tccagcgccg agatctcgcc gaaacggctg
     1201 accgccagct cgatagccgg aatcaggtcg ctgatggaga acggcttgac gaggtacgcc
     1261 atggcaccgg catcgcgtgc gcgctcgacg agctcacgtt ggctgaaggc ggtgagcacg
     1321 acgatcgggg cgatgcgctt gtctgcgatc tcggacgccg cgtcgatccc gtcccggcgc
     1381 ggcatcttga cgtccatgat caccaggtcg ggcctgagtt tctcggccaa ttcgacggct
     1441 tcctgcccgt cgccggcctc gccgaccacc tcgtaaccct cctcgcgaag catctcggca
     1501 agatcgagcc ggatgagggc ttcgtcctcc gcgatgagaa cgcggtgcgg cttggcatcg
     1561 tcagtcgttg tgccggtcat gggtgccatt gtggcggtcg tgggccgagt cagcgacctc
     1621 gggggatcct ctatggtgga aggccgcgcc ctcgtatccc aattggcaga ggaactggat
     1681 tcaaaaccca gccagtgtga gttcgagtct caccgagggc accgtgccct acatctcgct
     1741 acagcagaaa gctcgtcggc accggcgcca gcaacgccat ctgtactgcc ttggcctggc
     1801 ggtagcccgc gaatgcaccg atgagcgtga atccggcgat acccgcgacg ccgggcacgg
     1861 ctgcgacgag cagatcggtg ttggtggctg atcgcaggta ctccggatag ccg
//
LOCUS       DASYLX010000876         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_51582, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000876 DASYLX010000000
VERSION     DASYLX010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51582"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="tig"
                     /locus_tag="VGP27_20460"
     CDS             complement(<1..1331)
                     /gene="tig"
                     /locus_tag="VGP27_20460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV7853706.1"
                     /translation="MKSTVEQLSPTRVRINVEVPFTELEPDFDRAFAQLAQQVRLPGF
                     RPGKAPRKLLEARIGRGAVLQQVVNDALPSRYSEAVTTTEVRPLGQPDIEITKLEDNE
                     ELVFTAEVDIRPDITIPDLEALTIEVDPIEVSDEDVDSELQNLRARFGTLTGVERAAE
                     DGDFVSIDLSASVDGRDVPDAKTEGLSHEVGSGQLIDGLDEAIVGLKAGETKVFSTTL
                     AAGEHAGEEAQVTVTVNSVKERELPEPDDEFAQLASEFDTIDELKASLKEQVERVKRV
                     QQAEQIRDKALETLLDQIEVPLPEKIVQAQIDDAVHNAIHGLDHDEDAFATALEAQGS
                     SREEFDNDNKTNAERAVKTQLVMDAIADQLDVQVGQNDLTERLVLMSRQYGLEPQQLL
                     QYLQENNQLPVMFADVRRGLAVAAVVHRATVTDTEGNVIDTDEFFGPPATT"
     gene            complement(1378..1454)
                     /locus_tag="VGP27_20465"
     tRNA            complement(1378..1454)
                     /locus_tag="VGP27_20465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Pro,seq:tgg)
     gene            1547..1620
                     /locus_tag="VGP27_20470"
     tRNA            1547..1620
                     /locus_tag="VGP27_20470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Gly,seq:tcc)
     gene            complement(1699..2106)
                     /locus_tag="VGP27_20475"
     CDS             complement(1699..2106)
                     /locus_tag="VGP27_20475"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV7853707.1"
                     /translation="MGVAENLALHTRWVQAENSQDLSHQEDYLHDNVEVVQPGAELVV
                     GVAAYRAMMEDAYTALPDFNSVLDDQFATDDRVVSRWRLSGTHIAESFGFPARGKHIE
                     CPGMSVWEFEDGKARRGWIYFDLPSLAAQLGTP"
     gene            2507..3700
                     /locus_tag="VGP27_20480"
     CDS             2507..3700
                     /locus_tag="VGP27_20480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7853708.1"
                     /translation="MIDHSRVTVNGSCFPDFARVRDAFEANFRERNEVGAAIAVWVDG
                     ELAVNLWGGNADAAGRRRWRHDTLASIFSGSKALTSTCVHLLADRGELDLNDPVADYW
                     PEFAQAGKQDITLATVMGHRSGVIGPRTRLRPEETLDWDVVCDRIAKAEPWWQPGTAQ
                     GYHMVSFGFILGEIVRRVTGRTLGEYLRKEIAEPLGIDVHIGLPAAEHHRCADMVNKP
                     HIRDVLATGSAPGDPTTLKEHPLAAFSIAMGFIPDDELGSSDITAWRTAEFPATNAHV
                     SARGLATFYNALAQGKLLTHEHMEKCRISQGGFDTDFVLGPRVADHGWGLGYMLNQRG
                     MNGPNTKIFGHGGSGGSFGFVDLEHRVGYAYVMNNFDATKCNADPRSVALSNEVYSAL
                     GVTRD"
BASE COUNT          630 a         1237 c         1196 g          702 t
ORIGIN      
        1 tccgtggtcg cgggcgggcc gaagaactcg tcggtgtcga tcacgttgcc ctcggtgtcg
       61 gtgacggtgg cgcggtgcac gacggcggcg acggccaggc cgcgacggac gtcggcgaac
      121 atcaccggca gctggttgtt ctcctggagg tattgcagga gctgctgcgg ctcgaggccg
      181 tactgccgtg acatcagcac gaggcgctcg gtgaggtcgt tctggccgac ctggacgtcg
      241 agctgatcgg cgatggcgtc catcacgagc tgggtcttga cggcgcgttc ggcgttggtc
      301 ttgttgtcgt tgtcgaactc ttcgcggctg ctgccctggg cctccagcgc cgtggcgaag
      361 gcgtcctcgt cgtggtcgag gccgtggatg gcgttgtgga cggcgtcgtc gatctgcgcc
      421 tgaacgatct tctcgggcag cggcacctcg atctggtcga gcagcgtctc gagggccttg
      481 tcgcggatct gctcggcctg ctggacgcgc ttgacgcgct cgacctgctc tttgaggctg
      541 gccttgagtt cgtcgatggt gtcgaattcg cttgcgagct gggcgaattc gtcgtcgggc
      601 tcgggcagct cgcgttcctt gacggagttg acggtgacgg tcacctgagc ttcttcgccg
      661 gcgtgctcgc ctgcggcgag cgtggtgctg aagaccttgg tctccccagc cttgaggccg
      721 acgatcgctt cgtcgaggcc gtcgatgagc tggccggagc cgacctcgtg ggacaggccc
      781 tcggtcttgg cgtcgggcac gtcgcggccg tcgacggaag cggacaggtc gatggagacg
      841 aagtcgccgt cttcagcggc gcgctcgacg ccggtgaggg tgccgaagcg ggcacggaga
      901 ttctgaagct cggagtcgac gtcctcgtcg gacacctcga tggggtcgac ctcgatcgtg
      961 agcgcttcga gatcggggat ggtgatatcg gggcggatgt cgacctcagc ggtgaagacg
     1021 agttcctcgt tgtcttcgag cttggtgatc tcgatatcgg gctggccgag cgggcggacc
     1081 tcggtggtgg tgacggcctc gctgtagcgg ctgggcaggg cgtcgttgac gacctgctga
     1141 aggacggcgc cgcgaccgat gcgggcctcg agtagcttgc ggggcgcctt gccggggcgg
     1201 aaaccgggca gacggacctg ctgcgcgagc tgggcgaagg cccggtcgaa gtcgggctcc
     1261 agttcggtga agggcacctc tacattgatg cgaacccggg tggggctcaa ctgctcgacg
     1321 gtgctcttca cgtctggtgc tcctcagtag atctagtaac ggctcgggta gtgcttctgg
     1381 tcggggtgac aggatttgaa cctgcggcct tccgctccca aagcggatgc gctaccaagc
     1441 tgcgctacac cccgcggacc accgcagata ctacggccca ggcacgccga acctttaatt
     1501 ggatttggtt ggatggccgc cggtacagtg tggtgctgct gtactcgcgg gcgtagctca
     1561 atggtagagc cctagtcttc caaactagct acgcgggttc gattcccgtc gcccgctcca
     1621 ccagaccagc gcaatcgctg gagaacaggc gttcgacggc tcgcgcggta cctactttgt
     1681 acctactggc gtcagttttt aaggcgtccc aagctgtgcc gcgagcgagg gcaagtcgaa
     1741 gtaaatccaa cctcgccggg ctttgccatc ctcgaactcc cagacactca tgcccgggca
     1801 ctcgatgtgc ttgcctctgg cgggaaaccc gaatgactcc gcgatgtgcg ttccgctgag
     1861 ccgccatctg gacacgaccc ggtcgtcagt ggcgaactgg tcatcgagga cggagttgaa
     1921 gtcgggaaga gcggtatacg cgtcttccat cattgcgcgg tacgccgcga cgccgacgac
     1981 cagctcggcc ccgggctgaa ccacttcgac gttgtcgtgc aagtagtcct cttgatggga
     2041 tagatcctgg ctattctcgg cttggaccca gcgcgtgtgg agtgcgagat tctctgctac
     2101 gcccatgcct tcaatcgtgt cgcacctgat tgcatggtgt gcgccattcc ggccagctag
     2161 cacgggcgaa tgagcccgct accggcggct aatagctgtg atgtccggcg acgttgttga
     2221 cggtctagac cctacggaag aggagcacct ccggccttag tggagcaatg cggtgcctag
     2281 cggggccccg ctaacgtccg cgacaagctg ggctcgtagg cctcgcgcag tgctttccgg
     2341 cacagccgcg acaaatcagg cgacgccgcc gagcaagcac ccggaacctc agccgaatcg
     2401 caatcgaacc gcaaccgact ctcagcctcg gactgacagc cttgacaggc tgcgcccata
     2461 ccgtcggcgc ttcaggccat ccttggccgt cgtcgtcagg aggctcgtga tcgaccattc
     2521 gcgagtcacc gtcaacggtt cctgcttccc cgacttcgcg cgcgtccgtg acgctttcga
     2581 agccaacttc cgcgagcgca acgaggtcgg cgcagccatc gccgtctggg tcgacggcga
     2641 actcgccgtc aacctctggg gcggcaacgc cgacgcagcg ggccgacgaa gatggcgaca
     2701 cgacactctc gccagcatct tctccggctc aaaggcattg accagcacct gcgttcacct
     2761 cctcgccgat cgcggcgaac tcgacctgaa cgaccccgtc gccgactact ggcccgaatt
     2821 cgcccaagcc ggaaagcaag acatcaccct cgctaccgtg atgggtcatc gctccggcgt
     2881 catcggccct cgcacccgcc tcagacccga ggagaccctc gactgggacg tggtctgcga
     2941 ccgcatcgcc aaggccgaac cctggtggca gcccggcacc gcgcaggggt accacatggt
     3001 cagcttcggc ttcatcctcg gcgagatcgt ccgccgggtc accggccgca ctctcggcga
     3061 atacctccgc aaagaaatcg ccgagccgct cggcatcgac gtccacatcg gccttcccgc
     3121 cgccgagcat caccgctgcg ccgacatggt caacaagccc cacatccgcg acgtcctcgc
     3181 gacaggcagc gcaccgggag acccgaccac ccttaaagag catccgttgg cggcattctc
     3241 cattgcgatg ggcttcatcc ccgacgacga actcggctcc agcgatatca ccgcttggcg
     3301 cacagcggaa ttccccgcca ccaacgctca cgtgtcggcc cgcggcctcg ccaccttcta
     3361 caacgcgctc gcccaaggca agctgcttac ccacgagcac atggagaaat gccgaatctc
     3421 gcagggcggc ttcgacaccg acttcgtcct cggtccacgc gtcgccgacc acggctgggg
     3481 ccttggctac atgctcaacc aacgcgggat gaacggcccc aacaccaaga tcttcggcca
     3541 cggcggctcg ggcgggagct tcggtttcgt cgatctcgaa caccgcgtcg gctacgccta
     3601 cgtcatgaac aacttcgacg ccaccaagtg caacgccgac ccccgtagcg tcgcactctc
     3661 caacgaggtc tactccgctc tgggcgtaac ccgcgattag ttcagggccc cgaaaccgca
     3721 atttcctgtg agtacgctgg tcacatgaac ggcgtcttgc tcgtc
//
LOCUS       DASYLX010000877         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1477519,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000877 DASYLX010000000
VERSION     DASYLX010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477519"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            749..964
                     /locus_tag="VGP27_20485"
     CDS             749..964
                     /locus_tag="VGP27_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853709.1"
                     /translation="MQQNSDVYDAEGGNRLGPDTYFLKAGRTLELVAPCADDWCNLVI
                     PDPEVPGGKGFVYAGIDSGQNFLKLNQ"
     gene            complement(1026..1856)
                     /locus_tag="VGP27_20490"
     CDS             complement(1026..1856)
                     /locus_tag="VGP27_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853710.1"
                     /translation="MTATVDQAVEEAPSGGKVPLVELKSIGKSYGNITALKGISLRVH
                     AGEVTGILGDNGAGKSTLIKIIAGYHPQTDGDLLVDGESTRFTSPKDALGKGIATVYQ
                     NLAVVPLMPVWRNFFLGQEIRQSRFPWPLDANAMRATTLSELSKMGIDLPDVDVPIGS
                     LSGGQRQCVAIARAVFFGARVLILDEPTAALGVKQSGVVLKYITAAKDAGFGVVFITH
                     NPHHAHMVGDHFVLLNRGQQKLDCSYDDITLEHLTQEMAGGDELEALSHELRSAAKDG
                     "
     gene            complement(1860..2921)
                     /locus_tag="VGP27_20495"
     CDS             complement(1860..2921)
                     /locus_tag="VGP27_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853711.1"
                     /translation="MTTQATDLDVASHKIIRDERVKERNRLQRLLIRPEMGAAIGAVA
                     IFIFFLVVAPPFRSPEALATVLYASSTIGIMACAVALLMIGGEFDLSAGVAVTTSSLA
                     ASLLAYNLHLNLWAGAGLALIVALAVGFFNGYLVMKTKIPSFLITLGTLLMLTGINLA
                     ATKLISGQVATPSVNDMQGWASAQKVFSSSFSVFGVNVRITVFWWLLFTAIATYVLFK
                     TRIGNWIFAVGGDQESARAVGVPVTAVKIGLFMLVGFAAWFAGMHLLFAFNTVQSGQG
                     VGNEFIYIIAAVIGGCLLTGGYGTAIGAAIGAFIFGMVQQGIVYAGWNPDWFKFFMGV
                     MLLFAVIANNAFRNYAAKR"
     gene            complement(2944..3933)
                     /locus_tag="VGP27_20500"
     CDS             complement(2944..3933)
                     /locus_tag="VGP27_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7853712.1"
                     /translation="MRFSRLAAIAGASVLVFGVASCSSTGGKPQETGGGMGAGQANTP
                     RATVAMITHEVPGDSFWDLIRKGAQAAADKDNIELRYSNDPEAPNQANLVQSAIDSGV
                     KGIALTLAKPDAMAPAVKAAIDKNIPVVAFNSGFDNWKAMGVQEYFGQDEKLAGVAAG
                     ERLNQDGAKKVICVIQEQGQVALESRCAGVKEGFKGQSEILNVNSKDMPSVESTITAK
                     LQQDKSVDHVVALGAPIALAAVQSVGNAGSSAQVVTFDTNAALVDAIQKGDVKWAVDQ
                     QPFLQGYLAIDSLWLFINNKNLIGGGQATLTGPSFIDETNIDSVSELAKSGTR"
     gene            4088..4861
                     /locus_tag="VGP27_20505"
     CDS             4088..4861
                     /locus_tag="VGP27_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV7853713.1"
                     /translation="MTDISLAVCSEMVFTDMPLVDRVRRIDELGFDVEIWSWHDKDLD
                     ALAATGARFSSMTGYTHGDLIDPATADEVVRTAELSIKAAEKLGVARLNLHTAELVDG
                     HAARPRHRACGEMWLTAQRALERIGKLGEAAGVTFCVENLNTIVDHPGVPLARAKDTL
                     ALVEGVGHPNVRMMLDLYHAQIGEGNLIELIRRCGDAIGEVQIADVPGRCEPGTGEIN
                     YPAIAKALRDMRFVGTVGMEAWASADSEAALLAFRAAFT"
BASE COUNT          922 a         1583 c         1519 g          904 t
ORIGIN      
        1 ggctcggctg acgtcgtggc cctggtctgc gggcgggctg gcctcccgcc ggagcgactg
       61 accctggccg aagacttcct cttacctggt ggcgtcgcag tgccgccggc atcggcgtga
      121 cgtgcgccaa cggtctcgaa acgtagggaa acacccgata ccttcccagc attcagacgg
      181 ctaacgttca gctgaattgg gtgagaaacc cgcatgcacg gaaaggaaac acgatgaaac
      241 gagagactgc tcgccgagga tcaggcatct gtgccgccgc cgcgacggcc gtgagcgcgg
      301 ttggcttctc cctggcgtcg gcgtcaatgc tcccggtcgc ccatgccgcc tgcgaaaact
      361 ggattctcgg gccgacggta tgggagatcg tgctggacaa cggcgcccag atcgaaacct
      421 atggctggtc gggcaaatcc atgacgaaga cacccggcgg taagcccgct ttcgccgagt
      481 ggtttcctcc aggcggcggg gataagacgg agggaccgtc ctccggaagc atcaacgggc
      541 gcgcgatcaa cttcaccgca aactgggtca ccggtcccgg tgccggaggc cccgacacct
      601 tcaacggcac catcgacgac aacggggtgg cgagtggcat attcaacggc atgggctggc
      661 actccactgc aaggtcaagt gcgcgccggc tccggccgca atcccaccac cggcaggacc
      721 ggcgacgccc gcgaaggcga ccgtcaccgt gcagcagaac tccgacgttt acgacgccga
      781 aggcggcaac cggctcggac ccgatacata cttcctcaag gccggccgca cgctggaact
      841 ggttgcacct tgcgcagacg attggtgcaa cctcgtcatc cccgatccag aggtaccagg
      901 cggtaagggg ttcgtctatg caggtatcga ctcgggccag aacttcctca agctgaacca
      961 gtgactcatc gagcagcgtc cgccggcctc atcggtcgac ggacactgct ccgtgcgtaa
     1021 tgcggttagc cgtctttggc tgcgcttcgc agttcgtgtg acaacgcctc cagctcgtcg
     1081 ccgcctgcca tctcctgggt caggtgctcc agggtgatgt cgtcgtagct gcagtccagc
     1141 ttctgctggc cacggttgag cagcacgaag tggtcgccga ccatgtgcgc gtgatggggg
     1201 ttgtgcgtga tgaacacgac gccgaacccg gcgtccttcg ccgcggtgat gtacttcagc
     1261 accacgcccg actgcttgac gccgagcgcc gcggtgggct cgtcgagaat cagcacccgc
     1321 gcaccgaaga acacagcgcg cgcgatcgcg acacactgcc gctggccgcc ggacagcgat
     1381 ccgatgggca cgtcgacgtc aggcagatcg atgcccatct tcgacagctc cgaaagggtg
     1441 gtcgcccgca tggcattggc atccaacggc cacgggaacc ggctctgccg aatctcctgg
     1501 ccgaggaaga agttgcgcca taccggcatc agcggcacga cggccaggtt ctggtacacg
     1561 gtggcaatgc ctttgcccag tgcgtccttc ggcgatgtga acctggtcga ctcaccgtca
     1621 accagaaggt caccgtcggt ctgcgggtga tagccggcga tgatcttgat cagcgtcgac
     1681 ttgcctgcgc cgttgtcacc gaggatgccc gtcacctcac cggcatggac tcgcagcgag
     1741 atgcccttga gcgcagtgat attgccgtag gacttgccga tgctcttcag ctcgacgagt
     1801 ggcaccttgc cacctgatgg agcctcttcc acggcttggt caacagttgc agtcatgggt
     1861 cacctcttcg cggcgtagtt gcggaatgcg ttgttggcga tcaccgcgaa cagcagcatc
     1921 acgcccatga agaacttgaa ccagtcgggg ttccacccgg cgtacacgat gccctgctgc
     1981 accatgccga agatgaatgc accgattgcg gcgccgatcg ccgttccgta gccaccggtg
     2041 agcaagcagc caccgatgac ggccgcgatg atgtaaatga actcgttgcc gacaccctga
     2101 ccggactgca cggtgttgaa cgcgaacaac agatgcatgc ccgcgaacca ggccgcgaac
     2161 ccgaccaaca tgaagagacc gatcttcacc gcggtcaccg ggacgccgac tgctcgtgcg
     2221 ctctcctggt ccccaccgac ggcgaaaatc cagttgccga tgcgcgtctt gaacaacaca
     2281 tacgtcgcga ttgcggtgaa gagcagccac cagaacacgg tgattcgcac gttgacaccg
     2341 aataccgaga atgacgacga gaagaccttc tgcgccgaag cccagccctg catgtcgttc
     2401 acgctcggcg tcgcgacctg acccgagatg agcttggtcg ctgcgagatt gatacccgtc
     2461 agcatcaaca aggtgcccag ggtgatcagg aagctgggaa tcttggtctt catcacgagg
     2521 tagccgttga agaacccgac agccagggcg acgatgagcg ccaaccccgc gcccgcccag
     2581 aggttcaaat gcaggttgta ggccaacagc gacgcggcca gcgaactcgt ggttaccgcc
     2641 acgcccgccg acaggtcgaa ttccccgccg atcatcagaa gcgcgaccgc acacgccatg
     2701 atcccgatcg tcgagctggc atacagcacc gtcgccaacg cttcgggcga ccggaacggc
     2761 ggagcgacga ccagaaagaa aatgaagatc gccactgcac cgatcgccgc gcccatctcg
     2821 gggcgaatga ggagacgctg caaacggttt cgttccttga ctcgctcgtc acgaatgatc
     2881 ttgtgactgg cgacatccag gtcagttgct tgtgtagtca ttattcgctc tcttctgcac
     2941 ggcttaacgg gttccggact tggccagttc cgagaccgag tcgatgttgg tctcgtcgat
     3001 gaacgacggg cccgtcagag tggcttgacc gccaccgatc aggttcttgt tgttgatgaa
     3061 cagccatagg gagtcgatcg ccaggtatcc ctgcaggaac ggctgctggt ccaccgccca
     3121 cttcacgtcg cccttctgga tcgcgtcgac gagcgccgcg ttggtgtcga aggtgaccac
     3181 ctgcgcggag ctgccggcgt taccaacgga ctgcaccgct gccaaggcaa tcggcgcgcc
     3241 aagtgcgacg acgtggtcga cggacttgtc ctgttggagc ttggcggtga tcgtcgactc
     3301 caccgacggc atgtccttgc tgttgacgtt caggatctcg gactgccctt tgaagccctc
     3361 tttcacaccg gcgcagcgtg attcgagtgc gacctggccc tgctcctgaa tgacgcagat
     3421 gaccttcttg gcgccgtcct ggttcagtcg ttcgcccgcc gcgactcctg cgagtttctc
     3481 gtcttggccg aagtactcct gaacgcccat cgccttccag ttgtcgaagc cggagttgaa
     3541 cgcgacgaca ggaatgttct tgtcgatcgc agccttgacc gccggtgcca tcgcgtccgg
     3601 cttggccagc gtcagggcga tgcctttgac gccactgtcg atcgcgctct gcaccaggtt
     3661 cgcctgattc ggcgcctctg ggtcgttgga gtagcgcagc tcgatgttgt ctttgtcggc
     3721 tgcagcctgg gcacccttgc ggatcaggtc ccaaaacgag tcgccgggca cctcgtgcgt
     3781 gatcatcgca acggtcgcgc ggggggtgtt ggcctggccg gcgcccattc cgccgccggt
     3841 ctcctggggc ttgcctccgg tggacgaaca cgacgcgact ccgaatacca gcacactcgc
     3901 cccggcgatt gccgcgagcc gactgaacct cattgtttct ccttgccgtt gagtgctacg
     3961 taacacgcct caccggtcga tgtaatacgg ctcacagcag aatgtcaata ctttgttcgg
     4021 acattaggac tgcatatcaa atgttagtgt tcatcggtcc aggtcgagac gagatgggtg
     4081 acaagcaatg actgacattt cgctggcggt ctgctcggag atggtgttca ccgatatgcc
     4141 gctggtcgac cgggtccggc gcatcgacga gctcggcttc gatgtggaga tctggagctg
     4201 gcatgacaag gacctcgacg cgctggcagc gaccggcgcc cggttctcgt cgatgaccgg
     4261 ctacacgcat ggcgacctta tcgaccccgc gaccgccgac gaggtcgtgc gtaccgccga
     4321 gctgagcatc aaggccgccg aaaagcttgg cgttgcgcgg ctcaacctgc acaccgccga
     4381 actcgtggac ggccatgccg cccggccccg tcaccgtgcc tgcggcgaga tgtggctgac
     4441 ggcgcagcgc gcactggaac ggatcggcaa actcggcgag gcggccggtg tcaccttctg
     4501 cgtggagaac ctgaacacca tcgtggacca tcctggcgtg ccactcgcca gggcgaagga
     4561 cactctggct ctcgttgagg gtgtcgggca tcccaatgtg cggatgatgc tcgacctgta
     4621 ccacgcgcag atcggcgagg gcaatctcat cgagctgatc cggcgctgcg gggacgcgat
     4681 cggcgaggta cagatcgccg acgtgccggg ccgttgcgag ccgggcacgg gtgagatcaa
     4741 ctaccctgcc atcgcgaagg cattgcgcga catgcggttc gtcggcacgg tcggcatgga
     4801 ggcctgggcg tccgccgaca gtgaagccgc cctgttggcg ttcagggcgg ctttcaccta
     4861 ggtacagaac ctcaggcggc agcgctcacc gccagccctt tgccgtcctg cgcctcgaat
     4921 cgctcttc
//
LOCUS       DASYLX010000878         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3233071,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000878 DASYLX010000000
VERSION     DASYLX010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233071"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGP27_20510"
     CDS             complement(<1..881)
                     /locus_tag="VGP27_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853714.1"
                     /translation="MVKPERRTRADLVAAAAIALVVVVGAALIWWTSDARATISRPAA
                     SPVKTLAPAKVVPATLRQLWTAPSPKTTKPVLAGGSVVTGDGHHVDGRDPASGTVLWT
                     YARDLDLCGVTSVYNYAVAVYPDSRGCGQVSTIDGQTGVRGPSRTAYADPEVALSSDG
                     TTVLAAGDSRLELWRSDMVRMISYGALDARIKPGVPGPVLCRLESAAASTAAVSVLES
                     CPEAPDLRLTVLRPAKEEDTPDLKYVPLPGVKADTGARVVAVSETTTAVYVPTPRPSL
                     MIIDETGSTTANLPLPKP"
     gene            complement(899..2365)
                     /locus_tag="VGP27_20515"
     CDS             complement(899..2365)
                     /locus_tag="VGP27_20515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7853715.1"
                     /translation="MTALNPSFAKLGVRDEIVRALLEDGKEHTFAIQELTLPLAMAGD
                     DLIGQARTGMGKTLAFGVPLLHEITTDTDRPLTGAPRALIVVPTRELCLQVYGDLAGA
                     AKYLTVGDRKLSVVSIYGGRAYEPQTDALRAGADVVVGTPGRLLDLAQQGHLQLGGIS
                     VLVLDEADEMLDLGFLPDIERILRLTPDTRQSMLFSATMPDPIITLARSFMNQPTHIR
                     AESPQSSAVHTTTDQFAYRAHALDKVELVSRILQVRGRGATMIFTRTKRTAQKVSDEL
                     AERGFNVGAVHGDLNQGAREKALKAFRTGEIDVLVATDVAARGIDIDDVTHVINYQCP
                     EDEQAYVHRIGRTGRAGKTGVAITLIDWDEVARWQMIDKALGLGHPDPAETYSSSAHL
                     YEELDIPAEVTGSIGPSRPAKAAKRAPRDAAEAPARTRTKSRRRTRGGKPASGHPETD
                     NAAPTADSADTPAAADGEQPTRRRRRRRPRKAAAATAN"
     gene            2600..>3279
                     /locus_tag="VGP27_20520"
     CDS             2600..>3279
                     /locus_tag="VGP27_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEV7853716.1"
                     /translation="MSSTQPAASAEHIATSAASGVSADHPGVNELFALLAYGEVAAFY
                     RLTEEARMAPNLAGRINMASMAAAEMAHYELLRDELQRRGIDTVAAMTRYAPALENYH
                     RLTTPSTWLEALVKTYVGDALAADFYLEIADVLPDDVSAVVRAVLAETGHSQFVVAEV
                     RAAVTGSQRQRHRLALWSRRLLGEAITQAQFVLADRDELVDLVVSGTDGLGQLTQFFD
                     RLQHTHSTR"
BASE COUNT          438 a         1100 c         1191 g          550 t
ORIGIN      
        1 ggcttgggca gcggaaggtt ggccgtcgtc gacccggtct cgtcgatgat catcagcgac
       61 ggcctcggtg tcgggacgta gacggcggtg gtggtctcgg ataccgcgac cacccgggcg
      121 ccggtgtccg ccttcacgcc cggcagcggc acgtacttga ggtcgggggt gtcctcctcc
      181 ttcgccggcc gcagcaccgt cagccgcagg tcgggcgcct cagggcagga ctcgagcacc
      241 gacaccgcgg ccgtgctggc cgccgcggac tccagccggc acagcaccgg gcccggcacg
      301 ccgggcttga tccgggcgtc cagcgcgccg tagctgatca tccggaccat gtcggagcgc
      361 cacagctcga gtctgctgtc gcccgctgcg agcaccgtgg tgccgtccga cgacagcgcg
      421 acctcgggat cggcgtatgc ggtgcggctc gggccgcgca caccggtctg cccgtcgatg
      481 gtgctcacct gaccgcatcc ccggctgtcg gggtagacgg cgaccgcgta gttgtacacc
      541 gacgtgacgc cgcacaggtc gaggtcgcgt gcgtaggtcc acagcaccgt gccgctcgca
      601 ggatcgcggc cgtcgacatg gtggccgtcc ccggtcacca cgctgccgcc cgccagcacc
      661 ggcttcgtcg tcttcgggct cggcgccgtc cacagctggc gcagcgtggc gggcacgacc
      721 ttcgccggtg cgagggtctt caccggcgac gcggccggtc tgctgatcgt ggccctggcg
      781 tcgctggtcc accagatcag ggcggcgccg acgacgacga cgagcgcgat cgcagcggcg
      841 gccaccaggt cggcccgggt gcgccgctcg ggtttgacca tcgggtggtt cggcccagct
      901 agttggcggt cgccgcggcg gccttgcgcg ggcgacggcg acgacgcctt cgggtcggct
      961 gctcgccgtc ggcggcggcc ggggtgtctg cgctgtcggc cgtcggcgcc gcattgtcag
     1021 tctccgggtg gccgctcgcc ggcttgccgc cgcgggtgcg gcgacgcgac ttggtgcgcg
     1081 tgcgggccgg cgcctcggcg gcgtcacggg gcgcacgctt ggccgccttg gccggcctgg
     1141 acgggccgat cgagcccgtc acctcggcgg ggatgtccag ctcttcgtac aaatgcgctg
     1201 agctggaata ggtttcggcc gggtcggggt ggccgagacc cagcgccttg tcgatcatct
     1261 gccagcgcgc gacctcgtcc cagtcgatga gggtgatcgc gacgcccgtc ttgcccgccc
     1321 ggccggtgcg gccgatgcgg tgcacgtagg cctgctcgtc ttcggggcac tggtagttga
     1381 tgacgtgggt gacgtcgtcg atgtcgatgc cgcgggccgc cacgtcggtg gcgaccagca
     1441 cgtcgatctc gccggtgcgg aacgccttga gggccttctc gcgggcgccc tggttcaggt
     1501 cgccgtgcac cgcgccgacg ttgaagccgc gctcggccag ctcgtcggag accttctgcg
     1561 cggtgcgctt ggtgcgggtg aagatcatgg tggcgccgcg accccggacc tgcagtatcc
     1621 ggctgacaag ttccaccttg tcgagcgcat gcgcgcggta ggcgaactgg tcggtggtgg
     1681 tgtgcacggc cgacgactgc ggtgactccg cgcggatgtg cgtcggctgg ttcatgaagc
     1741 tgcgggccag cgtgatgatc gggtccggca tcgtcgccga gaacagcatc gactgccgcg
     1801 tgtcgggggt caggcgcagg atgcgctcga tgtcgggcag gaagccgagg tcgagcatct
     1861 cgtcggcctc gtcgagcacc agaaccgaga tcccgccgag ctgtagatgg ccctgttgag
     1921 cgaggtcgag gaggcggccc ggagttccga cgacaacgtc ggcgcccgcg cgcagtgcgt
     1981 cggtctgagg ctcgtaggcc cgcccgccgt agatcgacac caccgacagc ttgcggtcac
     2041 ccacggtgag gtatttcgcg gcgccggcga ggtcgccgta cacctgcagg cacagctcgc
     2101 gcgtgggcac cacgatgagc gcacggggcg cgccggtcag cgggcggtcg gtgtcggtgg
     2161 tgatctcgtg cagtagcggc acgccgaacg cgagggtctt gcccatgccg gtgcgggcct
     2221 gcccgatgag gtcgtcaccg gccatcgcca gcggcagagt cagttcttga atggcaaatg
     2281 tgtgctcttt gccgtcttcg agcagtgcgc gcacgatttc gtcgcgtacg ccgagtttgg
     2341 caaatgaggg gttgagtgca gtcatgcttg ttaggcgaag cctttccggt ccgttttctc
     2401 gtcggtcata attcgcgtcc acgcgcgcac gagtatgacg agaaaacttc gcgggccctg
     2461 cgctgagcta ggcgggccga ctgcgtgcac gcacatttct taggtagcgg ctcaggtgtt
     2521 tgcagccacc gcctgcttac atgataccgg taggtttgcc ctccggtcgc ccagccggtc
     2581 gccgtctaca gttgggccca tgagttcgac gcagcctgcg gcatcagcgg agcacatcgc
     2641 cacctcggcg gcctcaggcg tgtcggccga ccatccgggc gtcaacgaac tgttcgcgtt
     2701 gctggcctac ggggaggtgg ccgccttcta ccggctcacc gaggaggcgc ggatggcgcc
     2761 caacctggcc ggccggatca acatggcaag catggcggcg gcggagatgg cgcactacga
     2821 gttgctgcgc gacgagctgc aacgccgtgg catcgacaca gtcgcggcga tgacgcggta
     2881 cgcgcctgcg ctggagaact atcaccggct gaccacgccg agcacctggc tcgaagcgct
     2941 ggtgaagacc tacgtcggcg atgcgctggc ggccgacttc tacctggaga tcgccgacgt
     3001 gctgcccgac gacgtctcag cggtggtgcg cgccgtgctg gccgagacgg gtcactcgca
     3061 gttcgtcgtc gcggaggtcc gtgcggcggt gaccggcagc cagcggcagc gtcaccgctt
     3121 ggcgctgtgg tcgcgccggc tgctcggcga agccatcacc caggcgcagt tcgttctcgc
     3181 cgaccgcgac gaactcgtcg acctggtcgt ctcgggcacc gacgggctgg gtcagctgac
     3241 gcagttcttc gaccgcctcc agcacacgca cagcacccg
//
LOCUS       DASYLX010000879         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3125311,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000879 DASYLX010000000
VERSION     DASYLX010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125311"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1099
                     /gene="ssd"
                     /locus_tag="VGP27_20525"
     CDS             32..1099
                     /gene="ssd"
                     /locus_tag="VGP27_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEV7853717.1"
                     /translation="MNGRAGVLAMLAETAMRDDVDRVAAAAGVAVVHVAQPPSRQAWT
                     GASAVLLDAAGAGSCSAPSLPRRTHVIMLSSAEPGAADWHGAIAVGADRVMTLPADEA
                     ELVAALSEAAAYGDRDADRRGAVLAVMGGRGGAGASLFATALAQSARDALLVDADPWS
                     GGIDLVAGSEDKPGLRWPDLALQHGRLDFAALRQALPRIGGLSVLSSVRGGNDVEPGP
                     LAAVVDAGRRGGATVVCDLPRRLTRAVEFAVDTADLVVLVTTADIRGCAATNAVAAAL
                     SAINPNVGLVVRGPSPAGLRPADVARTVELPLLAAMRAQPPLAAMLERGGLRIGHRSP
                     VTAAARRVLAVLARHPLADVA"
     gene            1096..2262
                     /locus_tag="VGP27_20530"
     CDS             1096..2262
                     /locus_tag="VGP27_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEV7853718.1"
                     /translation="MSVSLIDRVRERLAAESAPLHPKVVAAAIRAESGGVLGDTEVLS
                     NLRMLQTELTGVGILEPLLRAQGTTDVLVTAPDAVWVDDGNGLRRSSIRFADEDAVRR
                     LAQRLASSVGRRLDEAQPWVDGQLTGVTADRFTVRLHAVLAPIASGGTCLSFRVLRPA
                     TQDLASLTKAGAIAAEAAGLLDRIIAARLAFLVSGGTGAGKTTLLSAVLGEVSPAERI
                     VCVEDAAELEPQHPHLVKLVARCANIEGVGEITVRELVRQALRMRPDRIIVGEVRGAE
                     VVDLLAALNTGHDGGAGTVHANSPAEVPARLEALAALGGLDRFALHSQLAAAVQVLLH
                     VGRDRSGQRRLDEIAVLRREQNGLVGVATAWHAASGFGSGADDLMRLLDERNAL"
     gene            2259..3026
                     /locus_tag="VGP27_20535"
     CDS             2259..3026
                     /locus_tag="VGP27_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853719.1"
                     /translation="MTVAALALALALMVAPSRHRMHRAPATRRRLPALAMAVALAAVS
                     LVLPVAATLAVCILLGTLALRRRRSAARCARAEEAAFLQSALDVLIGELRVGAHPVVA
                     FDVAAGEADGTVAKGLRAVAARARLGADVAAGLRSFAASSRLPSHWERLALFWGLAQT
                     HGLAIATLMRAAHRDIVERERFSARVDAGMAGARTTAAVLAGLPVLGIGLGQLIGADP
                     LSFLLSRGAGGWLLVTGVTLACCGLVWSDRITGRVLS"
BASE COUNT          406 a         1064 c         1212 g          533 t
ORIGIN      
        1 cccggtcgga tagcgcccgc attgtgggca catgaacggt cgtgccggcg tactcgcgat
       61 gcttgccgaa acggcgatgc gcgacgacgt cgaccgtgtc gccgccgctg cgggcgtggc
      121 tgtcgtccat gttgcgcaac cgccgagccg gcaggcgtgg acaggggctt cggcagtgct
      181 gctcgacgcc gccggtgccg gcagttgttc ggcgccctcg ctgcctcggc gcacgcacgt
      241 gatcatgttg agcagcgcgg aaccgggggc cgccgactgg catggcgcca tcgccgtcgg
      301 cgccgaccgc gtcatgacgc ttcctgccga cgaggccgaa cttgtcgccg cactgtcgga
      361 ggcagctgct tacggcgacc gcgacgccga tcgcaggggc gcggtgctcg cggtgatggg
      421 aggccgcggc ggcgccgggg cgtcgctgtt cgccaccgca ctcgctcaat ccgcgcgtga
      481 cgcgctcctc gtcgacgccg acccgtggag cggcggcatc gatctggtcg caggcagtga
      541 ggacaagcca gggttgaggt ggcccgatct ggccttgcaa cacggccgtc tggactttgc
      601 cgcgttgcgg caggcactgc cgcgcatcgg tgggttgagc gtgttgtcca gtgtccgcgg
      661 aggcaatgac gtcgagcctg ggccgctggc cgcggtggtc gatgcagggc gacgtggcgg
      721 cgccacggtc gtctgcgatc tacccaggcg gctgacgcgt gcggtcgaat tcgccgtcga
      781 cacggcggat ctggttgtgc tggtcaccac cgcggatatc cggggctgtg ccgcgaccaa
      841 cgccgtcgca gcggcgttga gcgcaatcaa cccgaacgtc ggtctggtcg tgcgcggtcc
      901 ctcgccggcc gggctgcgac ccgcggacgt ggcgcgcacc gtcgaactgc cgcttctggc
      961 ggccatgcga gcacaacccc ctctcgcggc gatgctggag cgcggcggtc tgcggatcgg
     1021 tcatcgctca ccggtgaccg ccgcggcgcg gcgggtgttg gcggtgttgg cacggcatcc
     1081 actggcggac gtcgcatgag cgtctcgttg atcgatcgcg tccgtgagcg gttggcggcc
     1141 gaatcggcac cccttcatcc caaggtcgtc gccgccgcga tccgagctga gtcgggcggt
     1201 gttctcggcg acaccgaggt gctgagcaac ctgcggatgc tgcaaacgga gctgaccggc
     1261 gtcggcatcc tggagccgct gctacgcgcg caaggaacta cggatgtgtt ggtcaccgca
     1321 cccgacgccg tatgggtcga cgacggaaac ggattgcggc gcagttccat ccggttcgcc
     1381 gacgaggacg cggtgcggcg gctggcgcag cggctcgcgt cgagcgtggg ccgtcgactc
     1441 gacgaagcgc agccatgggt cgacggccag ctgaccggcg tgaccgcgga tcggttcacc
     1501 gtccggctgc acgcggtgct cgcgccgatc gcctccggcg gtacgtgtct gtcgttccgc
     1561 gtgctgcggc ccgccacaca ggatcttgcg tcgctcacca aggccggggc gatcgccgct
     1621 gaggcagccg ggctgctcga caggatcatc gcggcccggc tggccttcct ggtctccggc
     1681 ggcaccggcg caggcaagac aacgttgttg tccgcggtgc tgggcgaggt ctcacccgcc
     1741 gagcgcatcg tctgcgtcga ggacgccgcc gaattggagc cgcaacatcc gcatctggtg
     1801 aagctggtcg cgcgatgcgc gaatatcgaa ggggtgggcg agatcaccgt tcgcgaactg
     1861 gtgaggcagg cgttacggat gaggccggac cggatcatcg tcggtgaggt tcggggcgcc
     1921 gaggtggtcg atctgctggc cgctctcaac accggtcatg acgggggcgc ggggaccgta
     1981 catgcgaaca gtcccgcgga agtccccgca aggctcgagg cgctcgcagc gctcggaggt
     2041 ctcgaccgct tcgcgttgca cagtcaactc gcggcggcgg tgcaggtgct gttgcacgtc
     2101 ggtcgggatc gatccggtca gcgcaggctc gatgaaatcg cggtactgcg ccgcgaacaa
     2161 aacggcttgg tcggggtcgc gacggcgtgg catgccgcca gcggattcgg aagcggcgcc
     2221 gacgatctga tgcggttgct ggacgagcgg aacgcgctgt gacggtcgcg gcgctggcgc
     2281 ttgcgttggc acttatggtc gcaccgtcgc ggcatcggat gcaccgcgcg ccagccactc
     2341 gtcgccggct tcccgcactg gcgatggccg tcgcactcgc cgcggtgtcg ctggtgctgc
     2401 ccgtcgcggc cacgctcgcc gtgtgcatcc tcctcggcac gctggcgttg cgccggcgac
     2461 ggtcggccgc ccgatgcgct cgcgcggagg aagcggcctt cttacagagc gcgctggacg
     2521 tcctcatcgg tgagctgcgc gtcggcgcgc accccgtggt cgcgttcgac gtcgcggcgg
     2581 gtgaggcgga cggcaccgtg gcaaagggcc tgcgcgcggt cgcggcacga gcgcgcctcg
     2641 gtgccgatgt ggcggccggg ctgcgcagtt tcgccgcatc atcgcggctg ccctcgcact
     2701 gggagcggct ggcattgttt tgggggctgg cgcagacgca cggcttggcg atcgcgacgc
     2761 tgatgcgggc cgcccaccgc gatatcgttg agcgcgaacg gttctcggcc cgtgtcgatg
     2821 ccggaatggc gggtgcccgg acgacggcgg cggtgttggc cggactaccg gtgctcggca
     2881 tcggcctcgg ccaactcatc ggtgcggacc cgctgagctt cctgttgtcg cgcggtgccg
     2941 gcggctggct gctggtcacc ggggtgacgc tggcctgctg cgggctggtg tggtcggatc
     3001 gcatcaccgg ccgggtgctc tcgtgagcac tcgagcggga gccgaggcat gagcgcagcg
     3061 gcgttgttgc tggcgacagc gctgctggtt gccggcagcc cgtcacgggc ccggatccgc
     3121 gtggcggtgc ccaccccggc ggtggcggtt tcgctcccgg gcgactcgga tgcgctggcc
     3181 gtcgcatcca cgctagacgt gttcgccgcc tgcct
//
LOCUS       DASYLX010000880         7918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3562717,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000880 DASYLX010000000
VERSION     DASYLX010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3562717"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1600
                     /locus_tag="VGP27_20540"
     CDS             107..1600
                     /locus_tag="VGP27_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEV7853720.1"
                     /translation="MAYRVRDLLDTPHLRLTLKGGGRGLDTPVTWAQTSDLEEPWSYL
                     AGGELLMKNGQTLPESPRGQTALIRAVAENGMCGMVIGLDAATPALTPAALDIADTLG
                     FPIMVVPYSVGFSAIGRAVADAAGSGRVAVTERVYNVLRQSVTTPGPANVLAQLERDL
                     SCRIAVLDASTGTVALEASDPPPDELRRVITTEISGRRGAIPGVLHVSTGAQRALVVE
                     VPDEEPTVLATYAFRGPTTDVVHLQHLASAVAVLLAQQNARREHERRIGAEVMGQLCD
                     GRISDREGDRELGERGLDAATCCVVAADGVSAASERQLHLGLGRRSVPHLLLRRATLL
                     YALMPVTEWALDVLGQRLGGQATLGISEPLSRPSRAPGAVREATWAARVAVTTSRRTA
                     RFADATMFSVLRDPEEARVVVDRVLGRLIAYDETHSGNMVSTLDAYLRCERSWLRTAR
                     ELGIHRQTVVYRIQRVEEITGRKVTDTATLAEFWLALRARDLLAVPS"
     gene            complement(1565..2374)
                     /locus_tag="VGP27_20545"
     CDS             complement(1565..2374)
                     /locus_tag="VGP27_20545"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047789 - creatininase activity [Evidence
                     IEA];
                     GO_process: GO:0046449 - creatinine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HEV7853721.1"
                     /translation="MSTDTDPRVYQDMTWEEISAAARDDVPIVIPIGATEQHGRHLPV
                     CTDWVLPQRVLCDAARVRDIVVGPFLPFGYRSRPGSGGGQHFPGTVSLRATTFMSVLE
                     DVLSELKRGGFRNIVLYNWHYENSGFIYEPAFLVSEHNPELKIVVVEDVFPEFTDERK
                     KAIWPAGFPGLALEHAAVIETSLWMYHDESAVRTDRMAPDAPQRVVNHDVVPIDTRMS
                     TASGSLSSPMGASAEVGKLLNEWLVQRVVAIVDDEFSQRQSARDGQQVSRA"
     gene            complement(2511..3944)
                     /locus_tag="VGP27_20550"
     CDS             complement(2511..3944)
                     /locus_tag="VGP27_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7853722.1"
                     /translation="MTEPVSAPPSQSRLEKLGYSQQLVRRLNIPEVVGLAMADVSPTM
                     AVLFLSAGVFVIGGTFSIGAGLILSVVVVLISLCLAELAGMFPIAGGMYSLVRQVLPA
                     PFSWITMFNYLIQGVVIPASIALGMVGFLRDLFPGLGFPDTLIAIVLLALATGIALTK
                     IEVGAWATATMVIVECIVLGIVTVAALAHPHQNLAEVTFHPTVLSNGELVAVGFAVML
                     ATLAPAFNVINGYDAALGFSEELKGGERNIAKAVIISAVLACVLIMVPLISAVVAAPD
                     LKEFFSDPAPVVYSVQASLGDKARVIVDVGVSIALFNAMLSLLMYFGRGVYTTGRDGM
                     WPAAVNRAVGSINRFRVPGVGILCLAVPAAVLVFTSALNFLIIFAGTVIAAVYFCIGL
                     AAFWSRRSMPDEPRPYKMPLWPLPPLVVIVFTGIALATQETQYLVAEVILIVLALLAW
                     AGSKKWSPRPAPSGSEEPGTRPLDPIS"
     gene            complement(3941..4765)
                     /locus_tag="VGP27_20555"
     CDS             complement(3941..4765)
                     /locus_tag="VGP27_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853723.1"
                     /translation="MPEMGGRIALVTGAASGIGLASAEALARQGAQVALLSRPDDDLD
                     AAAHRVRAHGGKVMTVGADVADSAAVLDAFQRVESELGPLDAIHNNAGISIVSPLADT
                     TDEVFRRLVDVNLAGSFYVLREAARVMQPRRAGAIVNTASELAIIGQAGYVAYTATKG
                     AILAMTRSAAAELASWGIRVNAVCPGTTKTPMFLAEFDGAADPAAELADNAASVAMQR
                     IAEPSEIAEAVVFLLSDRAGYITGTHLIADGGRTACVAAGSIGVATPSPTTTGGLS"
     gene            complement(4758..5672)
                     /locus_tag="VGP27_20560"
     CDS             complement(4758..5672)
                     /locus_tag="VGP27_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7853724.1"
                     /translation="MTTLVTGGRGFVGRHLVDQLITEGGKVVSYNRDFSADERDGLTV
                     VQGELFDIPRLIETLRRYNVDRIIHTAGQSHPGVSIELPWTTFKANAEGTLAVYEAAR
                     ATGVRRVVNFSSECALGNTPLGAKATERVTPTPTTPYGVTKVAGELFGTVYNSLYDME
                     VVSLRITEVYGPGLWMPSLLGDMIRAGLRGDPFVLETGGEHPFQFVYVDDVATAARSA
                     STSTTLSQPVYNVSGGRQVTVSETARLLEQHIPGSRYAIGPGLLPNWDSQGPYDLTAS
                     RRDLGYTPAWSLELGIVKQIDWLRNDHA"
     gene            5780..7285
                     /locus_tag="VGP27_20565"
     CDS             5780..7285
                     /locus_tag="VGP27_20565"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV7853725.1"
                     /translation="MSLILRHVLEHPLLRRAQPSVLSGHDHLDHAVRWVHSADLYDIA
                     PLLRGDEMLLTNGVGLIGVDETARRLYVRRLAESGVAGLCFEVGRTFAEVPAEMVEEA
                     VDVGLPIVELQPVLRFTEVAEAVNSELIDRSVARLRHADEISRALSEALARGASLTEL
                     IERVAATLGTWARLSDYAGRIVVDAGQPVLDEEAPTSEAPVLVEGVAWGRLTVGTTGI
                     PRVLVEAIMDRAPTVLGLCLIREQKDVAGGLRAQQVLLHHLVDNDRADRTVLEAQLRA
                     AGVAVADHQYACIAVDPSRVESAAHIVDVIVRRAGSGVFGLIDGVLCGVVAGPASTTT
                     PNLAQLARHTALAELGSQSRLCATVSRTVRDIADLGRAMSETRATLGLGQELHMNEAV
                     ISVQALALERLLATHGDPDATRQFVEDQIGALTRADSAKNSQLSHTLEVLAACGGSKA
                     EAAKRLHVRRQSLYYRLDQISRLLDINVNDPKQLITLAVAITAERLMYAGK"
     gene            complement(7286..>7918)
                     /locus_tag="VGP27_20570"
     CDS             complement(7286..>7918)
                     /locus_tag="VGP27_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEV7853726.1"
                     /translation="GLAFDLLSSENEKVFTGHDNGVITLDLAEGDDVHREHLRISMDE
                     PYRTLLGHFRHEIGHYYYYRLVDPVPDYKKTFQGLFGDPDADYQDALDRHYQGGPPAD
                     WKKNYVSSYATMHAAEDWAETFAHYLHIRDTLDTAASFSIAPAAATFERRVLGPSGFD
                     QIIDMWLPLSWSLNMINRSMGKQDLYPFVLPPAVLEKMRFMHTVVEETTN"
BASE COUNT         1353 a         2578 c         2680 g         1307 t
ORIGIN      
        1 tctcaacgga catcgaacgg cttgccgtga gggccgcgaa ctggcgcttt gcgcccggcg
       61 gctttgcgag gcaacgacgt aaccacgtgt ttcgaacagg tgactgatgg cgtatcgagt
      121 tcgcgacctg ctggatacgc cgcaccttcg gctcaccctc aagggcggtg gccggggcct
      181 ggacacgccg gtcacctggg cgcagacttc ggacctcgaa gagccgtggt cgtatctggc
      241 cggcggcgaa ctgctgatga agaacggcca gacactgccg gaatccccgc gcggacagac
      301 cgcgctgatc cgcgcggttg cggagaacgg catgtgcggc atggtgatcg ggttggacgc
      361 ggccacccct gcgctcaccc cggccgcgct cgacatcgcc gacaccctgg gcttccccat
      421 catggtggtc ccctactccg ttggcttctc cgcgatcggg cgcgccgttg ccgatgccgc
      481 cggcagcggg cgcgttgcgg tgaccgagcg ggtgtacaac gtcctccggc agtcggtgac
      541 cactccggga ccggccaacg tcctcgctca actggaacgc gacctgtcgt gccggatcgc
      601 tgtcctcgac gcgtccaccg gcacggtggc actggaggcg tccgacccgc cgccggacga
      661 gctgcgccgc gtcatcacca ccgagatctc cggcaggcgg ggtgcgattc ctggagtgct
      721 gcacgtgtcg accggggccc agagggcgct cgtcgtcgag gtgcccgacg aagagccgac
      781 ggtgctcgcg acatacgcgt tccgtgggcc gacgacggat gtggtgcatc tgcagcacct
      841 ggcgtccgcg gtcgcggtgc tgctggcaca gcagaatgcg cgccgcgagc acgagcggcg
      901 catcggagcc gaggtgatgg gccagttgtg cgacggccgg ataagtgacc gcgaaggcga
      961 cagggaactc ggcgagcgcg ggttggacgc ggcgacctgc tgcgtggtgg cggcggacgg
     1021 cgtatcggcg gcgagcgagc gtcaactgca cctcggtctc ggcaggcgtt cggtgccaca
     1081 cctgctgttg cgccgggcga cgctgctgta tgccctcatg ccggtgacgg agtgggctct
     1141 cgatgtgctc ggtcagcggc tgggcggcca ggccacgctg ggcatcagcg agccgctgtc
     1201 gcggccgtcg cgcgcgccgg gcgcggtgcg cgaggcgacg tgggcggcgc gcgtcgccgt
     1261 cacgacatcg cgcaggaccg cccggttcgc ggacgcgacg atgttctcgg tgttgcgcga
     1321 tcccgaagag gcgcgtgtcg tggtcgacag ggtgctgggc cggctcatcg cctacgacga
     1381 aacgcattcc ggcaacatgg tttcgacgct cgatgcctat ctgcggtgtg aacggtcgtg
     1441 gctgcggacg gcgagggaac tcggcattca ccggcagacg gtggtctacc ggatacagcg
     1501 cgtcgaggag atcactggtc gcaaggtgac cgacaccgcg acgctcgccg agttctggct
     1561 ggcgctacgc gcgagagacc tgctggccgt cccgagctga ttgtcgttgc gagaactcgt
     1621 catcgacgat ggcaaccacc cgttgcacca gccattcgtt cagcagcttc cccacctcgg
     1681 cgcttgctcc catcggcgaa gatagggaac cgctcgccgt cgacatgcgg gtgtcgatcg
     1741 gcacgacatc gtggttgacc acgcgctgcg gcgcgtcggg cgccatccga tcggtccgca
     1801 ccgccgattc gtcgtggtac atccacagcg acgtctcgat caccgcggcg tgttcgagcg
     1861 ccaggccggg aaagccggcc ggccagatcg ccttctttcg ctcgtcggtg aactcgggaa
     1921 agacgtcctc gacgacgacg atcttgagct cgggattgtg ttcggacaca agaaaagccg
     1981 gctcgtagat gaatccgctg ttctcgtagt gccagttgta cagcacgatg ttgcggaagc
     2041 cgccacgctt gagttcgctc agaacgtcct cgagcacact catgaaggtg gttgcccgca
     2101 gcgacaccgt gccggggaag tgctggccgc cgccgctacc cggtcggctg cggtacccga
     2161 agggcaggaa tggcccgacc acgatgtcgc ggacacgggc ggcgtcgcag agcacccgct
     2221 gaggcagcac ccagtcggtg cacaccggca ggtggcggcc gtgctgctcg gtggcgccga
     2281 tgggtatcac aatcgggacg tcatctctcg cggcggccga gatctcttcc caggtcatgt
     2341 cctggtagac ccgcgggtcg gtgtcggtgc tcatctcaaa cttccctcct gtgaccggct
     2401 ttcggagccg tcgatgatct ccacgacgac gggaacccgg cacgtttcca tcaggtcccg
     2461 cgccgcagac ggcagtcggc gtacatgaaa ggtcgaccct tcggtagcgc tcaggagatc
     2521 gggtcgagcg gtcgtgtccc gggttcctcc gaacccgatg gcgcgggccg tggagaccac
     2581 ttcttggaac ccgcccatgc caacaacgcg aggacgatca gaatcacttc ggcgaccagg
     2641 tactgcgttt cctgggtggc gagcgcgatg ccggtgaaga cgatcaccac cagtggcggc
     2701 aacggccaca gcggcatctt gtagggccgt ggctcgtcgg gcatcgacct gcgactccag
     2761 aatgccgcca agccgataca gaaatacacc gcggcgatca ccgtgcccgc gaagatgatg
     2821 aggaagttca gcgcggacgt gaacaccagc acggccgcag gcacggcaag gcacagaatg
     2881 ccgacacccg ggacccgaaa cctgttgatg gagccgacgg cccggttcac cgccgcgggc
     2941 cacatgccgt cgcggccggt ggtgtagacg ccgcgcccga agtacatcaa cagcgacagc
     3001 atcgcgttga acagcgcgat gctcacgccg acgtcgacga tgacgcgggc cttgtcgccc
     3061 agcgatgcct gcaccgagta gacgacggga gcaggatcgg agaagaactc cttgaggtcg
     3121 ggtgcggcga ccaccgccga gatgagcgga accatgatca gcacacacgc cagcaccgcg
     3181 gagatgatga ccgccttggc gatattgcgc tcgccgccct tgagttcttc ggagaacccc
     3241 aacgccgcgt catagccgtt gatgacgttg aatgccgggg ccagcgtcgc aagcatcacc
     3301 gcgaaaccga ccgcgaccag ttcgccgttg ctcagcacgg tgggatgaaa ggtgacctcc
     3361 gccaggtttt ggtgcgggtg ggccaacgcc gcgaccgtca cgatgccgag cacgatgcac
     3421 tcgacgatca ccatcgtcgc ggtggcccac gcgccgacct cgatcttcgt cagtgcgata
     3481 ccggtggcca gcgccagcaa cacgatcgcg atcagggtgt cgggaaaacc gaggcccggg
     3541 aacaggtctc ggagaaaccc caccatgccc agcgcgatgc tggccggaat cacgacgccc
     3601 tggatgaggt agttgaacat cgtgatccag gagaacggtg ccggtagaac ctggcgcacc
     3661 agcgagtaca tgccgcccgc gatcgggaac atgcccgcca gctcggccag gcacagcgag
     3721 atgagcacca cgacgaccga aaggatcagg ccggccccga tcgagaacgt gccgccgatg
     3781 acgaacactc ccgcggacag aaacagcacc gccatggtcg gtgagacatc ggccatggcc
     3841 aggccgacga cctcgggaat gttcagccgc cgcacgagct gctgggagta gccgagcttt
     3901 tcgagccggg attgcgacgg cggcgctgac acgggttcgg tcatgagagt cctccggtgg
     3961 tggtgggcga tggtgttgcg acgccgatgg atccggcggc cacacaggcg gtgcggcctc
     4021 cgtcggcgat caggtgtgtg ccggtgatgt agccggcgcg gtccgacagc aggaacacga
     4081 cggcttcggc gatctcggag ggctcggcga tgcgctgcat ggccacgctg gccgcgttgt
     4141 ccgccagttc ggccgccggg tcggcggcac cgtcgaactc cgcgaggaac atcggtgtct
     4201 tcgtggtgcc cggacagacg gcgttgacgc ggatgcccca cgacgcgagt tcggcggccg
     4261 cgcttcgggt catggccagg atcgcgccct tggtggccgt gtaggcgacg tagccggctt
     4321 ggccgatgat cgccaattcc gaggcggtgt tgacgatcgc gcccgcgcga cggggttgca
     4381 tgacgcgcgc ggcctcccgc aggacgtaga acgacccggc gaggttgacg tccaccagcc
     4441 gccggaagac ctcgtcggtg gtgtccgcca gcgggctgac gatcgaaatg cctgcattgt
     4501 tgtggatggc gtcgagtggg cccagttcgg attcgacgcg ctggaatgcg tcgagaaccg
     4561 cggccgagtc cgcgacgtcg gcaccgaccg tcatcacctt gcctccgtgc gcgcgcaccc
     4621 gatgcgcggc ggcgtccagg tcgtcatcgg gccgggacag aagcgccacc tgggcgccct
     4681 gtcgggcgag cgcttccgcg ctggccaatc cgatccccga ggcggcaccc gtcaccaacg
     4741 cgatgcgacc gcccatctca ggcatggtcg ttcctgagcc agtcgatctg cttcacgatg
     4801 cccaactcca ggctccaggc aggcgtgtag ccgaggtccc gccttgaagc cgtcaaatcg
     4861 tagggcccct gactgtccca gttcggcagc agacccggcc cgatcgcgta gcggctgccg
     4921 gggatgtgct gctcgagcaa ccgcgcggtc tcgctgacgg tgacctggcg tccgcccgac
     4981 acgttgtaga ccggctggga cagcgtcgtc gaggtggacg ccgaccgggc cgcggtagcg
     5041 acgtcgtcga catagacgaa ttggaacggg tgctcgccac cggtctcgag aacgaaaggg
     5101 tcgccgcgca gtccggcgcg gatcatgtca ccgagcaggc tcggcatcca aaggccgggg
     5161 ccgtacacct ccgtgatgcg cagggagacg acctccatgt cgtagagcga gttgtagacg
     5221 gttccgaaca gctcgcctgc caccttcgtc accccgtagg gcgttgtggg agtcggcgtg
     5281 acacgctcgg tcgccttcgc gccgagcggc gtgttgccga gcgcacattc ggacgagaag
     5341 ttgacgaccc ggcgcacacc cgtggcgcgc gcggcttcat acacggcgag ggtgccctcg
     5401 gcgttcgcct tgaacgtcgt ccacggcagt tcgatggaca caccgggatg gctttggccc
     5461 gcagtgtgga tgatccggtc gacgttgtac cggcgcagcg tctctatcag acgcgggatg
     5521 tcgaacagct cgccttgaac gacggtcagg ccgtcgcgct catcggccga gaagtcgcgg
     5581 ttgtagctga ccaccttgcc gccttcggtg atcagttggt ccaccaggtg gcgcccgacg
     5641 aatccgcgcc cacccgtcac caaagtggtc atgtgcgctc ctcgagggtc ggcgtcgact
     5701 cagaatgcaa ccgactcgtc gcctgcggca acagacgcaa tgccaggtgt ttgacaccat
     5761 ttatgggtca caatgtccta tgagcttgat cctgcggcat gtgctcgaac atccgctgtt
     5821 gcgccgcgcg cagccctcgg tactcagcgg ccacgaccac ctcgatcacg ccgtccggtg
     5881 ggtgcacagc gccgacctct acgacatcgc gccactgttg cgcggcgacg agatgctgct
     5941 gaccaacggc gtcggcctga tcggtgtcga tgagaccgca cgtcggctct acgtccgccg
     6001 gcttgccgaa agcggcgtcg ccgggctgtg tttcgaggtc ggcaggacgt tcgccgaagt
     6061 cccggcggag atggtcgagg aagccgtcga cgtgggcctg ccgatcgtcg agttgcagcc
     6121 ggtgcttcgc ttcaccgagg tggccgaggc ggtcaacagt gagctcatcg acaggtcggt
     6181 ggcccggctg cgccatgccg acgagatctc gcgtgccctc tccgaggcgc ttgcccgcgg
     6241 cgcctcgttg accgagctta tcgaacgggt cgccgccacg ctcggcacct gggcccggct
     6301 cagcgattac gcaggcagga tcgtcgtgga cgcaggccag ccggtcctcg atgaggaagc
     6361 gccgacgtcg gaggcgcccg tcctggtcga gggcgtggca tggggtcggc tgaccgtggg
     6421 cactaccgga atcccacgcg tcctcgtcga ggcgatcatg gatcgtgcgc cgacggtgct
     6481 cggtctctgc ctgatccgcg aacagaagga cgtcgcgggc ggtctgcgcg cacaacaggt
     6541 cctccttcac cacctcgtcg acaacgatcg cgctgaccgg accgtcctcg aggcccaact
     6601 gcgcgccgcg ggtgtcgccg tggcggatca ccagtacgcc tgcatcgcgg tggacccctc
     6661 gcgcgtcgag tcggcggcgc acatcgtgga cgtgatcgtg cgtcgggcgg gcagcggggt
     6721 gttcggcctc atcgacggag tgctgtgcgg tgtggtggcc gggccggcct ccaccacgac
     6781 accgaatctc gcacagctgg cccgccacac cgctttggcc gaactggggt cacagtcacg
     6841 tctgtgcgca acggtcagcc gcacggttcg cgacatcgca gacttgggaa gggccatgtc
     6901 ggagacgcgt gccacgctcg gtctcggaca agagcttcac atgaacgaag cggtgatcag
     6961 cgtccaggcg ctcgctctcg agcgcctact ggccacgcac ggcgacccgg acgccacccg
     7021 gcagttcgtg gaggatcaga tcggcgccct cacaagagcc gattccgcga agaacagcca
     7081 actttcacac accctcgagg tgctggcggc ctgcggaggc agcaaagccg aagccgcgaa
     7141 gcgcctccat gtgcggcgcc aatccctgta ttaccgactc gaccagatat ccaggttgct
     7201 cgacatcaac gtcaacgacc cgaagcaact gatcaccctc gccgtggcga tcacggctga
     7261 acgattgatg tacgccggta agtgatcagt tggtcgtttc ctcgacgacg gtgtgcatga
     7321 accgcatttt ctccagcacc gccggcggca gcacaaacgg gtacaggtcc tgcttgccca
     7381 tcgacctgtt gatcatattg agcgaccacg acaacggcag ccacatatcg atgatctggt
     7441 cgaaaccact ggggcccagc actcttcgtt cgaatgtggc ggcagccggg gcgatgctga
     7501 atgatgccgc cgtgtccagt gtgtcgcgga tgtgcagata gtgcgcgaag gtctccgccc
     7561 agtcttcggc cgcgtgcatc gtggcgtagg acgacacgta gttcttcttc cagtcggccg
     7621 gtggaccgcc ctggtagtgg cggtccagcg catcctgata gtcggcgtct ggatcgccga
     7681 agagtccctg gaaggtcttc ttgtagtccg gcaccgggtc gacgagccgg tagtagtaat
     7741 agtggccgat ctcgtgccgg aaatgtccga gcagggtgcg gtaaggctcg tccatcgata
     7801 tgcgcaagtg ctcacggtgc acgtcgtcgc cttcggcgag gtccagtgtg atcacgccgt
     7861 tgtcgtggcc ggtgaacacc ttctcgttct cactggaaag caggtcgaac gccaagcc
//
LOCUS       DASYLX010000881         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1149157,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000881 DASYLX010000000
VERSION     DASYLX010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149157"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1135)
                     /locus_tag="VGP27_20575"
     CDS             complement(197..1135)
                     /locus_tag="VGP27_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEV7853727.1"
                     /translation="MTEPDDAGEVTAEPDDAGEVTAEPDDVDETTAEAEEPPADSGEG
                     PRRRARREREARREAQARAVAIEEARREAKRRVNGKSADDSKPLARGRVRGLKVLVWA
                     VVLVVLFVGLGLLLYFTPIMAARNVVITGVGTVTQEEVIAAAAVKDGTPLLQVNTDDV
                     AERVATIRRVASARVQREYPSTLRITVVERIPVVVKDYPDGPHLFDRDGVDFATAPPP
                     PNLPYLETENPGPGDPPTKAALEVMTALRPEVAGQVAKVSAPSVAAVTLTLVDGREVV
                     WGTNDRTEEKAQKLAALLTQPGHTYDVSSPDLPTVK"
     gene            complement(1132..2598)
                     /gene="murC"
                     /locus_tag="VGP27_20580"
     CDS             complement(1132..2598)
                     /gene="murC"
                     /locus_tag="VGP27_20580"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV7853728.1"
                     /translation="MSPGALPEELQRVHMVGIGGAGMSGIARILLDRGGLVSGSDAKE
                     SRGVAALRARGAEIRIGHDESSLDLLPGGPTAVVTTHAAIPKTNPELVEARRRGIPVI
                     LRPVVLAKLMDGYTTLMVTGTHGKTTTTSMLIVALQHSGFDPSFAVGGDLGEAGTNAH
                     HGSGDCFVAEADESDGSLLEYTPNVAVVTNIEADHLDFFGSAEAYGAVFDDFVERLAP
                     GGALVVCTDDSGAAALAERTAALGIRVLRYGSDDRPDLAGRLVSWEQQSTGAVAHIQL
                     AAEKHPRVMRMSVPGRHMALNALAALLAAMEIGAPAEKVLDGLAGFEGVRRRFELVGT
                     AHGVRVFDDYAHHPTEVRATLGAVRTVTEQSGRGRSVVVFQPHLYSRTATFAREFGEA
                     LDGADEVFVLDVYAAREQPMAGVSGATVAEHVGKPVHYVPDFSAVAELVANAVHSGDV
                     VVTMGAGDVTMLGPEIVAALQARANRSTPQRGPGAGAR"
     gene            complement(2595..3716)
                     /gene="murG"
                     /locus_tag="VGP27_20585"
     CDS             complement(2595..3716)
                     /gene="murG"
                     /locus_tag="VGP27_20585"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV7853729.1"
                     /translation="MNGPISVVLAGGGTAGHVEPAMAVADALKALEPDVRITALGTHR
                     GLENRLVPERGYHLELITPVPLPRKPSADLLRLPLRVRRAVKETRTVLDDVGADVVVG
                     FGGYVAVPAYLAARRRGVPVVVHEANASAGWANKLGARSARRVLSAVADPGLPRAEVV
                     GVPVRAAITSLDRIAERAKARAHFGFADDARVLLVFGGSQGARSINRAVAPAAKDLAA
                     AGVSVLHAHGPKNTLDLPEPADGDPPYVAVPYLDRMDLAYAAADLAICRSGAMTVAEV
                     SAVGLPAVYVPLPIGNGEQRLNALPVVNAGGGILVDDADLTPGFVGDTVAGLLGDAPR
                     LTAMTAAAAMAGHRDAAQKVAEVALEVARNGRSRRKSDR"
BASE COUNT          556 a         1367 c         1272 g          609 t
ORIGIN      
        1 ctcatcagcg atccccttca gccctggttg tctttcggag cgaagaaatt tgagcaaact
       61 ctaaacctca acgagagggt tatagttatg tcaagttact tcgcgcaaac agaacggtag
      121 ggcgaggtca agcactagcc atgcaggcgc gccgacggaa acgcccggaa tttcggtcag
      181 tgtcttggga caccgatcac ttgaccgtcg gcaaatcagg gctcgagacg tcgtaggtgt
      241 ggcccggctg cgtcagcagc gccgccaact tctgcgcctt ctcctctgtg cggtcgttgg
      301 tgccccacac cacttccctg ccgtcgacga gcgtcagcgt gaccgccgcc accgaaggcg
      361 ccgacacctt ggctacctgt ccggccacct ccggccgcag cgccgtcatc acttccagcg
      421 ccgccttggt cggcggatcg ccgggtccgg ggttttccgt ctcgaggtac ggcaggttgg
      481 gcggcggcgg cgcggtcgcg aagtccaccc cgtcgcggtc gaacagatgc ggcccgtcgg
      541 gatagtcctt gaccacgacg ggaatccgct cgaccaccgt gatccgtagc gtcgacgggt
      601 attcacgctg tacgcgcgcg ctggcgacac ggcggatggt ggccacccgc tcagccacgt
      661 cgtcggtgtt cacctgcaat agcggcgtgc cgtccttgac tgccgcggcg gcgatgacct
      721 cttcctgggt cacggtgccg acgccggtga tgacgacgtt gcgcgccgcc atgatcgggg
      781 tgaaatagag cagcaggccg agaccgacga acaggacgac cagcacgacc gcccacacca
      841 acaccttcag gccccgaact ctgccccgcg caaggggttt ggaatcatcg gccgacttgc
      901 cgttgacccg ccgcttggct tccctgcggg cttcctcgat cgcgacggcg cgggcctgtg
      961 cctcgcggcg cgcttcgcgt tcccgcctgg cccggcgccg cgggccctcc cccgaatctg
     1021 cgggcggttc ctcggcctcg gccgtcgtct cgtccacatc gtccggttca gccgtcacct
     1081 cgccggcgtc gtccggttca gctgtcacct cgccggcgtc gtccggctcg gtcaccgtgc
     1141 acccgctccc gggccccgct gcggcgtcga gcggttggct cttgcctgaa gcgccgcgac
     1201 gatttccggg ccgagcatcg tcacgtcgcc cgcgcccatc gtgacgacca catcacccga
     1261 atgcactgcg ttggccacga gttcggcgac ggcggagaag tccggcacgt aatgcaccgg
     1321 cttgccgacg tgttcggcaa ccgtcgcccc gctgacaccg gccatcggct gctcccgcgc
     1381 ggcgtacacg tcgagcacga agacttcgtc ggcgccgtcc agtgcctcgc cgaattcgcg
     1441 agcgaatgtg gctgtgcgtg agtacaaatg gggctggaac accacgacgg agcgaccgcg
     1501 gccactctgc tcggtgaccg tccgcacagc accgagggtg gcgcggactt cggtcgggtg
     1561 gtgggcgtag tcgtcgaaca cccgaacgcc gtgcgccgtg ccgacgagtt cgaagcgacg
     1621 ccgcaccccc tcgaacccgg ccaggccgtc gagcactttc tcggcgggcg cgccgatctc
     1681 catcgcggcc agcagcgcgg cgagcgcgtt gagtgccatg tgcctgcccg gcaccgacat
     1741 tcgcatcacc cgcgggtgct tctcggcggc cagttgaatg tgcgccacgg ctccggtgct
     1801 ctgctgttcc cagctcacca gcctgcccgc caggtcggga cggtcgtcac tgccgtagcg
     1861 cagcacccgt atccccaggg ccgcagtgcg ttcggcgagt gcggcggcac ccgaatcgtc
     1921 cgtgcagacc accagtgcgc cgcctggtgc tagccgctcg acgaagtcgt cgaacactgc
     1981 gccgtaagcc tcggcgctgc cgaagaagtc gaggtggtcg gcctctatgt tggtcaccac
     2041 cgcgacattc ggcgtgtatt cgagcagtga gccgtcgctc tcatcggcct cggcgacgaa
     2101 acagtcaccg ctgccgtggt gggcgttggt gcccgcctcg ccgagatcgc cgcccacggc
     2161 gaacgacgga tcgaatccgc tgtgctgcaa cgcgacgatg agcatcgacg tcgtggtggt
     2221 cttgccgtgc gtgccggtca ccatcagggt ggtgtagccg tccatcagct tggccagcac
     2281 caccggtcgc agaatcaccg ggatgccgcg acgcctggcc tcgacgagtt cggggttcgt
     2341 cttcgggatc gccgcatggg tggtgaccac cgccgtcggt ccgcctggca gcaggtccag
     2401 tgaggactcg tcatggccga tgcggatctc ggcgcccctg gcgcgcaatg ccgcgacgcc
     2461 acgtgactcc ttggcgtcgg agcccgacac cagcccgccg cgatccagca ggatccgcgc
     2521 gatacccgac atgcccgcgc cgccgatgcc gaccatgtgc acccgttgca actcctcggg
     2581 cagcgcgccg gggctcaacg gtcggacttt cggcgcgaac gcccgttgcg tgccacctcc
     2641 agggcgacct cggccacctt ctgcgcggcg tcgcggtggc ccgccatcgc ggcggcggcg
     2701 gtcatcgccg tcagccgcgg agcgtcgccg agcaacccgg ccaccgtgtc gccgacgaac
     2761 ccgggcgtga ggtcggcgtc gtcgacgagg attccgccgc ccgcattgac gacgggcagc
     2821 gcgttcagtc gctgctcgcc gttgccgatg ggcagcggca cgtacactgc cggcagtccg
     2881 acggcggaga cctcggccac cgtcatcgcg ccggaccggc agatcgccag atccgctgcg
     2941 gcgtaagcga ggtccatccg atcgaggtac ggcacggcca catacggcgg gtcgccgtcg
     3001 gcgggctcgg gcaagtcgag ggtgttcttg ggcccgtgtg cgtgcagcac cgaaactcca
     3061 gccgcggcaa ggtctttggc tgcgggtgcg acggcccggt tgatcgaccg cgcaccctgc
     3121 gagccgccga acaccagtag cacccgcgca tcgtcggcga agccgaagtg agcgcgggct
     3181 ttcgcccgct ccgcgatccg gtcgagcgat gtgatcgcag cgcgcaccgg cacgccgacc
     3241 acttcggcgc ggggcagacc gggatccgcg acggcggaga gcacccggcg cgccgaccgg
     3301 gcgccgagct tgttggccca gcccgcgctg gcgttggcct cgtgcacgac caccggcacc
     3361 ccgcgacggc gcgccgccag gtacgcgggc actgcgacat acccgccgaa cccgacgacg
     3421 acgtcggcgc cgacgtcgtc gagcaccgtc cgcgtctcct tgacggcgcg gcgcacccgc
     3481 agcgggagac gcagtaggtc agcggagggt ttccggggca gcggcaccgg cgtgatgagc
     3541 tcgaggtggt agccgcgttc gggcaccagc cggttctcca ggccacggtg ggtgcccaga
     3601 gcggtgattc gcacgtcggg ttccagcgct ttgagcgcat cggcgacggc catcgcgggt
     3661 tcgacgtggc ctgccgtccc gccgccggcg agaactacgg agatcggccc attcacccgt
     3721 gacgttgacc ttccagtgtg cgagcccgtc ggccctggtg gcgctgttgt ccagcggcgc
     3781 tctgcttgac acgctcctcg cgtc
//
LOCUS       DASYLX010000882         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2138345,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000882 DASYLX010000000
VERSION     DASYLX010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138345"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGP27_20590"
     CDS             <1..426
                     /locus_tag="VGP27_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853730.1"
                     /translation="ALMTVLTIPRLGAERADRQYRIRTIGESGAPLAFGSDWPVSSGA
                     PFDGIAVAVSRQTADGEPAGGWTPAEVVPIERALTAYTAGVAYQAFAENSWGRLVPGA
                     SADLLWCNRDPRTTAALELPALDVRATYLRGRPAYSATD"
     gene            440..1804
                     /locus_tag="VGP27_20595"
     CDS             440..1804
                     /locus_tag="VGP27_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7853731.1"
                     /translation="MSSTASADSTVPPPSQEDEGHLRRVLGTPSLVLFGMVYMVPLTV
                     FTTYGIVTETTGGRLSVAYLVTLAAMVFTARSYARMAAAYPVAGSAYTYTQKTFGAPL
                     GFLAGWSLLLDYLFLPMLNYLVIGIYLNAAIPAIPPWLIIVVTIAIVTVLNIVGIVSV
                     DRANFVIIAVQAIFIVVFIILACVTISGSGTVDFMAPLKGDGSAPGFNPILAGAAILC
                     LSFLGFDAVSTLSEEAKDPRRTVPRAIMIATVASGIIFVVLAYVSQLVFPSNKFADVD
                     SGSLDVMTTAGGQFLNTFFTAAYVAGALGSAITSQASVARILYAMGRDGILPRKIFGH
                     VSSRFSTPDYAILVVSVISLLAVVIDLATLASIVSFGALVAFSAVNLTVIKHYFIDQG
                     EKNVLNNLVLPAIGFLLTVYLWFSLSGLTLIVGLIWLAVGFLWLLVVTRGFQRPTPVL
                     ALEE"
     gene            complement(1834..2628)
                     /locus_tag="VGP27_20600"
     CDS             complement(1834..2628)
                     /locus_tag="VGP27_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853732.1"
                     /translation="MPVIKTVFITGAAAGIGRATALTFARNGYTVGGYDVDEVGLKSL
                     AAEIETLGGRAVTGHLDVTDPDEMAQRLGEFADAAGGRLNVMINNAGILRAGRFEDIA
                     LSAQLKEIDINTKGVVNGLHAAFPYLRATPNPVVVNLASASAIYGQPELATYSATKFF
                     VRGITEALDVEWDRYGIRVIAMWPLYVKTAMTENVRTGTTESLGIRLTAQDVADAIVA
                     AVEPSLLRRGIHQVHFPVGSQTKASALTARLPLWLQRLINKRIADM"
     gene            complement(2632..2823)
                     /locus_tag="VGP27_20605"
                     /pseudo
     CDS             complement(2632..2823)
                     /locus_tag="VGP27_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873309.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3136..3423
                     /locus_tag="VGP27_20610"
     CDS             3136..3423
                     /locus_tag="VGP27_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853733.1"
                     /translation="MSSTQRYLILGLATAAVPFTIVVASPAMADCTSAGSATVCAQGT
                     VRGGGPTAPIAGPAYPSYCSDPWYCDNGWDMDVIIGRPGRPGGGGGGIGPR"
     gene            3465..>3586
                     /locus_tag="VGP27_20615"
     CDS             3465..>3586
                     /locus_tag="VGP27_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
                     /protein_id="HEV7853734.1"
                     /translation="MFFRSVIGAGMIAGSMLVGTTAIAAADPPNCTAADLAGVMS"
BASE COUNT          555 a         1145 c         1167 g          719 t
ORIGIN      
        1 gccctgatga ccgtgctgac gattccgcgc ctcggcgccg agcgcgcgga caggcagtac
       61 cggatccgca ccatcggaga gtccggtgcg ccattggctt tcggctccga ctggccggtg
      121 tcctccggtg caccgttcga cggcatcgcc gttgcggtgt cgcggcagac cgccgacggc
      181 gagcccgcgg gcggatggac gcccgccgag gtggttccga tcgaacgggc gctcaccgcg
      241 tatacggcgg gagtggcgta tcaggcgttc gccgagaaca gctggggccg gctcgttccc
      301 ggtgccagtg cggatctgct ctggtgcaac cgcgacccca ggaccactgc ggcgctggaa
      361 ctgccggcgc tcgacgttcg tgccacgtac ctgcgcggca ggccggccta ctcggccacc
      421 gattagagga gagaacgaaa tgtcgtcaac agcgtccgcc gactccactg tgccgccgcc
      481 ctcacaggag gacgaaggcc accttcgccg ggtactcggc acaccgtccc tggtgttgtt
      541 cggcatggtt tacatggtgc cgctcacggt gttcaccacc tacggcatcg tcaccgagac
      601 gaccggcggc cgcctttcgg tcgcgtacct cgtcactctg gccgcgatgg tgttcacggc
      661 gcgttcgtat gcccgcatgg cggccgccta tcccgttgcc ggctcggcat atacgtacac
      721 gcagaagacc ttcggcgcac cgctgggctt cctcgccggc tggtcactgc tgttggatta
      781 cctgttcctg ccgatgctga actacctcgt catcggcatc tacctgaacg cggcgattcc
      841 ggcgataccg ccttggttga tcatcgtcgt caccatcgcg atcgtgaccg tgctcaacat
      901 cgtcggcatt gtgtcggtgg accgggccaa cttcgtgatc atcgcggtac aggcgatctt
      961 catcgtggtc ttcatcatct tggcgtgcgt gaccatctcg ggctcgggca ccgtcgattt
     1021 catggccccg ctgaagggcg acggcagcgc acccggtttc aatcctatcc tcgccggcgc
     1081 ggccatactg tgcctttcgt tcctcggatt cgacgctgtg tcaacccttt ccgaggaggc
     1141 gaaggaccca aggcgcacgg tgccgcgcgc catcatgatt gccaccgttg catcgggcat
     1201 catcttcgtc gtcctggcgt acgtctcgca gctggtcttc ccgtcgaata agttcgccga
     1261 cgtcgactcc ggctcgctgg acgtgatgac gaccgcgggc gggcagttcc tgaacacctt
     1321 cttcaccgcc gcgtacgtgg cgggcgcact cggatccgcc atcacctcgc aggcgtcggt
     1381 agcgcgcatc ctctacgcga tgggccgcga cggcatcctg ccgcgcaaga tcttcggcca
     1441 tgtgtcgtct cggttcagca cgcccgacta cgcgattctg gtggtgtccg tgatttcgct
     1501 actggccgtc gtgatcgatc tggcgaccct ggcgtcgatc gtcagtttcg gtgcgctggt
     1561 ggccttctcg gcggtgaacc tgacggtgat caagcactac ttcatcgatc agggcgagaa
     1621 gaatgtgctg aacaacctgg tgctcccggc gatcggcttc ctgttgaccg tctacctgtg
     1681 gttcagcctg tccggactca ctctgatcgt cggcctgatc tggctggcgg tcggcttcct
     1741 gtggctgctc gtggtgaccc gtggcttcca gcgtccgacg ccggtgctcg ccctggagga
     1801 gtagctcggt cagacggtga agtgaccaaa gggtcacatg tcggctatcc gcttgttgat
     1861 cagccgctgc agccagaggg gaaggcgcgc ggtgagcgcc gacgccttgg tctgcgaacc
     1921 gacgggaaaa tgcacttggt ggatcccgcg ccggagtagg gacggctcga ccgccgccac
     1981 aatcgcgtcg gcgacgtcct gcgcggtcag ccggatgccg agggattcgg tcgtgcctgt
     2041 cctgacgttc tcggtcatcg cggtcttcac atacagcggc cacatggcga tgacgcggat
     2101 gccgtaccga tcccattcga cgtcgagagc ctctgtgatg ccgcggacga agaacttcgt
     2161 cgcgctgtag gtggcgagtt cgggctggcc gtagatcgcc gaggcggacg cgaggttgac
     2221 cacgaccggg ttcggggtgg cccgcaggta ggggaacgcg gcgtgcagtc cgttcacgac
     2281 acccttggtg ttgatgtcga tctccttgag ttgggcggag agggcgatgt cctcgaagcg
     2341 gccggcccgc aggatgcccg cgttgttgat catgacgttg aggcgcccgc cggccgcgtc
     2401 ggcgaactcg ccgagccgtt gcgccatttc gtcgggatcc gtcacgtcga ggtgcccggt
     2461 gaccgcccgg ccgccgagcg tctcgatctc ggcggccagc gatttgagtc ccacctcgtc
     2521 gacgtcgtaa ccgccgacgg tgtagccgtt acgcgcgaac gtgagcgcgg tggcgcgtcc
     2581 gatccctgct gctgcgccgg tgatgaagac ggtcttgatg acgggcatag gtcatgatcg
     2641 catcgtggcc aggttgtgcc acaggccctt gaggctgtcg gtgccgccga acagggtggt
     2701 gaagtgggtc gagtcgatca ggacgatgtc gccggagcgc gctccgttcg gcggcatcca
     2761 gatcagcgcg ttgaactcgg tgttgccctc agcggtgaac ggatgcggcc atgatcagtc
     2821 gatcgtcata cccgcccctg cgttttggag acccgatggt gtgcgaacct actccggtgg
     2881 tccggcccca cggccgtttc gcgcccaaag tcgaagtgtc cagatcgcga acgacagcgc
     2941 acagcattgt tcacctagcg gtgcgaagat atttgcccgg tttcgccaga gttgatcgat
     3001 gacaggtatg tcggacaagg caattcagga agaaaatctc gctaagttgc taatctgccc
     3061 tctcgctgtg aaaggcagaa catactccgg tgtatgggtc gctcggccct accgaattgc
     3121 agagagggag cagccgtgtc atcaacgcag cgctacctga tattgggatt ggcgacggcg
     3181 gccgtcccgt tcacgatcgt cgtagcttcg ccggccatgg cggattgcac cagcgccggg
     3241 tcagccaccg tgtgcgcgca ggggacggtc cgcggcggtg gtccgaccgc tccgatcgcg
     3301 gggccggcat acccctcata ttgctccgac ccgtggtatt gcgacaacgg ctgggatatg
     3361 gacgtgatca tcggacggcc cggtcgtccc ggcggcggcg gaggtggtat cggtccgcgc
     3421 tgagcatcgc catgtcttgt aaattactta gtcaaggagc caccatgttc ttccgtagcg
     3481 tgattggcgc cggcatgatc gccggctcaa tgctcgttgg taccaccgca attgcggccg
     3541 ccgatccgcc gaactgcacc gccgccgacc tcgccggcgt catgtc
//
LOCUS       DASYLX010000883         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2685393,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000883 DASYLX010000000
VERSION     DASYLX010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685393"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..2081)
                     /locus_tag="VGP27_20620"
     CDS             complement(39..2081)
                     /locus_tag="VGP27_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7853735.1"
                     /translation="MRQGPLAGRYPAVAAMVTFALIPYLALSAAMDPLVPIISSQLHI
                     SAQTMSLSSGLGNAAYAVGTVLAVQFAQHLPQRRMLVLYAVLLVVGSVLAAAAANGAM
                     FVGGHVLQGLATSMLLIAAAPPLTIGFPRDKLRNTAVIMNMCVFGAVALGPFIGGAQA
                     EAQAWRPLFWIVAGIALVALILAALTFDDAPPADLDAPRDLPAIALATVGCTAAFIGA
                     AQLTSHSFGDAAATLPMLGGLILIVVLIVYQFRARRPLLTIRTMLTSAIPVAGVGVAL
                     FAAAASVAATALTANVLLQTYNPVRVGLLYLPELGGAIVMAVVFGFVISRRAMHYLPL
                     VGMALLAAGILVYRMALPANQPLALLGAGLTGLALGATVAPALFVAGFSLQSASLQRV
                     FAIIELLRAVAAFMVAPILAHIADALPGDLNAGTGITLWIGFGLAVGGAVFGVAVYAL
                     SGARPQTPDLDEFLDGESPAWYSPPLLARLRNLPTSIPVTKPTPVTASADAAFRPASA
                     GGPVLLAYDGTELAACAIRMAAAQLAPYRDALVVCVWQTVDVGFTPSDTTHFDADQAT
                     DVRRAAERTAACGADLAARAGLRSRSMAIEAAPTWKGIVRAADENAASMIVLGPHRRS
                     GLLGHLQGSVAAAVVTHTDIPVLLIPEQPCRAPSAQFGGPAESVTGTLRGLPARST"
     gene            2360..3220
                     /locus_tag="VGP27_20625"
     CDS             2360..3220
                     /locus_tag="VGP27_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV7853736.1"
                     /translation="MALWQREKGTSGEVGTVQTFKRDVGAEMEIAVTEASTLEFQIAL
                     ASQPGTQTIESLEFRCNGGQVDAQEIIGEHGTRIHRFAASVGTVTASYQATILGRAEP
                     AAVRDLDMSTYLRPSRYAEADKFFGFASTEFGQYADSVTILEMVSSWVGTRLHYVPGS
                     SDPIDGAADTLLAGSGVCRDYAHLVIALLRAVNVPARLVAVYAPGCDPMDFHAVAEAF
                     VEGAWHVVDATCLAPRQSMVRIATGRDAADTAFLDNHKGSITLNHMEVRAIVDGDLPR
                     DSVNELVELN"
     gene            3558..3947
                     /locus_tag="VGP27_20630"
     CDS             3558..3947
                     /locus_tag="VGP27_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853737.1"
                     /translation="MSTDDASGTTANDLGGADDMLSPMEATDSDEVHNDDGDEVVDPP
                     EGWSGVTKVGASTGEDRDGETLDGRLAQEVPDVTPDDVDPRDADEPGDRDEAVLGSDA
                     APDDPGVHRGQIDGAPEDGESLFPVVE"
     gene            4139..4879
                     /locus_tag="VGP27_20635"
     CDS             4139..4879
                     /locus_tag="VGP27_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7853738.1"
                     /translation="MGAAQRIQVREATQDGVTVLLVDGVLDSSTYLELRDRIVKAALD
                     ETLAVIVNISALRVPAPSAYAVFTSARWQVSRWPDVPVLLVCGHSTGRTALRRQGITR
                     YVPVHDSLDAALAALDDVGNWVRRRRSAELAAGPESVVLAGELVTEWLWDWGHSDKIA
                     TAKVIVIELVKNVYAHTDSTAELRVEANGSSVTVAVSDGSGRLASRIESTYETFRPSG
                     LHFVAALAHTWGCTPSTGGKTVWATIAR"
     gene            5138..5545
                     /locus_tag="VGP27_20640"
     CDS             5138..5545
                     /locus_tag="VGP27_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV7853739.1"
                     /translation="MAKYLLLKHYRGAPAAVNDVPMAQWAPEEIEAHIKYMNDFAARL
                     EGTGEYVDSQALAPDGAWVRYDGEGRPPVTDGPFAETKDLIAGWMILDVDTYQRAVEL
                     AGELSAAPGAGGKPIHEWLEVRPFLTASPTVTE"
     gene            5545..6720
                     /locus_tag="VGP27_20645"
     CDS             5545..6720
                     /locus_tag="VGP27_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV7853740.1"
                     /translation="MAAAAVDEQQLRDLIPGVLAALVHRGADFATAEDAVQEALVRAV
                     ETWPAQRPEDPKGWLITAAWRRFVDLTRSDIARHRRETRVSDQPAQGPTAAVDDTLSL
                     YFLCAHPSLTPASAVALTLRAVGGLTTRQIAQAYLVPEATMAQRISRAKRTVSAVRLH
                     RPGNLGTVLRVLYLVFNEGYSGDVDLAAEAIRLARQLALISDDPEVAGLLALFLLHHA
                     RRSARTRSDGSLVPLAEQDRCVWRRDLVAEGVAVLQTALARDRLGEYQAQAAIAALHA
                     DAQTAAETDWVQIVEWYDELVRLTDSPVVRLNRAVAVGEADGPQAGLAALADLDPTLP
                     RYTASAAYLHARAGDTAMAADLYAAAAAQAQNIAERNHLTLRAATLRQRLSSSQDGE"
     gene            6788..7012
                     /locus_tag="VGP27_20650"
     CDS             6788..7012
                     /locus_tag="VGP27_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853741.1"
                     /translation="MTSNAERGEVSQLAAQVGWQHRDAERSDYYLRRPERVHIVWQGN
                     QAISGGALYRDNILMSISRELDTVKGWLNR"
     gene            7023..>7426
                     /locus_tag="VGP27_20655"
     CDS             7023..>7426
                     /locus_tag="VGP27_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV7853742.1"
                     /translation="MMLSAQLWSDNADVASEVLGNPFVRGISDGSLPRELFAGYIAQD
                     AFFLESFARAYALALARSSDTATLLTLADLLGGVRQELALHASYAGSWGIDMAGVTPR
                     PATLAYTEFLLATASTQDLGVIYAAMTPCMRL"
BASE COUNT         1285 a         2456 c         2517 g         1168 t
ORIGIN      
        1 ctcgacgggc accaccccgg tgatgtcgtg acactgactt acgttgaccg cgcgggcaat
       61 ccgcgcagtg tgcccgtgac gctctctgcc ggccctccga actgagccga cggcgcccgg
      121 cacggctgct cggggatgag caggacggga atgtcggtgt gcgttacgac ggctgccgca
      181 acgctgccct gcaggtggcc gagcaggccg ctacggcggt gaggtccgag cacgatcatg
      241 ctggcggcgt tctcgtcggc ggccctgacg atgcccttcc acgtcggcgc cgcttcgatc
      301 gccatgctgc gggaccgaag gccggcccga gccgcgaggt cggcgccgca ggctgcggtc
      361 cgctcggcgg cgcgacggac gtccgtggcc tggtctgcgt cgaaatgcgt tgtgtcgctt
      421 ggcgtgaacc cgacatcgac cgtctgccat acgcacacga cgagggcatc tcgatagggc
      481 gccagctgcg ctgcggccat ccgtatcgca cacgcggcga gttcggttcc gtcgtaggcc
      541 aacagcaccg gcccgcctgc tgacgccggt cggaaagcgg cgtctgccga cgcggtgacc
      601 ggcgtcggtt tcgtcacggg aatcgaggtc ggcaggttcc gcagcctggc cagcagcggc
      661 ggtgagtacc aggcaggcga ttcgccgtcg aggaactcat ccagatcggg tgtctgcggg
      721 cgagcaccgc tcagcgcgta caccgcgaca ccgaagaccg ctccgccgac ggccagaccg
      781 aaaccgatcc acagcgtgat acccgtgccc gcattgaggt cacccggcaa cgcgtccgcg
      841 atatgggcga ggatcggcgc gaccatgaac gcggccaccg cgcgcagcag ctcaatgatc
      901 gcgaaaacgc gctgcaggct tgcggattgc agcgagaaac ctgccacgaa caacgcgggg
      961 gcgaccgttg cgcccagcgc aagaccggtc agcccggcgc cgagcagcgc caggggctgg
     1021 ttggcgggca gcgccatccg gtacaccagg atgccggccg ccaacagggc catgccgaca
     1081 agcgggaggt agtgcatcgc gcgccgtgag atcacgaacc cgaagacgac ggccatcaca
     1141 atggcgccgc cgagttcagg caagtagaga agcccgaccc ggacggggtt gtaggtctgc
     1201 aacagcacgt tggccgtcag ggcggtggcc gcgaccgacg cagcggcggc gaacagtgcc
     1261 actccgacac cggcgaccgg gattgcgctc gtcagcatcg tgcggatggt cagaagcgga
     1321 cggcgggcac ggaactgata gacgatcagc accacgatga ggatcaggcc gccgagcatc
     1381 ggcagtgtcg ccgcggcgtc accgaagctg tggctggtga gctgcgcggc gccgatgaac
     1441 gcggccgtgc agccgacggt cgccaacgcg atggcgggca ggtcgcgcgg agcgtcgaga
     1501 tcggcgggcg gtgcgtcgtc gaaggtcaat gccgccagaa tcagagccac cagcgcgatg
     1561 cccgccacga tccagaacag cggccgccag gcttgcgcct cggcctgcgc gccgcctatg
     1621 aatggcccga gtgcgacagc cccgaagacg cacatgttca tgatcacggc ggtattgcgg
     1681 agcttgtccc tcggaaagcc gatcgtcagc ggcggcgcgg ccgcgatcag aagcatgctc
     1741 gtggccaacc cctgcagaac gtgtccgccg acgaacatcg cgccgttggc cgcggcggcg
     1801 gcaagtaccg acccgacgac gagcagcacc gcgtagagaa ccagcatcct gcgctgcggg
     1861 aggtgttggg cgaactggac cgccagcacg gtgcccaccg cataggccgc gttgcccaac
     1921 cccgagctca gactcatcgt ctgagcgctg atatggagtt gagacgaaat gatcggcacc
     1981 agcggatcca tcgcggccga caacgccagg tagggaatca gagcgaaagt gaccatggcg
     2041 gccactgccg gataccgtcc ggcgagagga ccttggcgca tcaggcgtgc tcgacggtct
     2101 gggtgtggtt tgtcgcgcgg cgacgcgcag aatcgaggca gacggttagc gggatcgcgg
     2161 caggcacgtg tcccgggaac aggttcatac atggtcctat tcccattgac ggccgcaaat
     2221 ccttgattgt caggaagcgc acagcaacgg agcgacttcc gccggccccg attaggcttg
     2281 ggcatgtcat cgccaacggc tcagcacgac tgccgtcacg acctgtggtg acggcgtcct
     2341 tcaatgaaag tggttcggca tggctctgtg gcagcgcgag aagggtacct ccggcgaggt
     2401 cggtaccgtg cagacgttca agcgcgacgt cggggcggag atggagatag cggtcacgga
     2461 ggcgagcact ctcgagttcc agatcgcgct tgcgtcgcag cccgggacgc agacgatcga
     2521 gtcgctcgaa ttccgttgca acggaggaca agtggatgcg caggagatca tcggcgagca
     2581 tggcacgcgc atccaccgct tcgccgcctc tgtcggcacg gtcacggcgt cctaccaagc
     2641 cacgatcctg gggcgagccg aaccggcggc cgtgcgcgat cttgacatgt ccacctacct
     2701 gcggccgagc cggtatgccg aggcggacaa gttcttcggc tttgcctcca ccgagttcgg
     2761 tcagtacgcc gattcggtga cgatcctgga gatggtgtcg tcgtgggtcg gcacgcgcct
     2821 gcactatgtg cccggatcga gcgacccgat cgacggagcg gccgacacat tgctcgccgg
     2881 ttccggggtc tgccgcgact acgcgcacct cgtcatcgcg ctgctgcgcg cggtcaacgt
     2941 cccggcacga ttggtcgcgg tctacgcgcc gggttgtgac ccgatggatt tccacgccgt
     3001 ggcggaggcc ttcgtcgagg gagcgtggca tgtggtggac gccacgtgtc tggcaccgag
     3061 acagtcaatg gtccgcatcg ccaccggccg cgatgccgcc gacaccgcct tcctggacaa
     3121 ccacaaaggt tcgatcacgc tcaatcacat ggaggtgcgg gcaatcgtcg acggcgatct
     3181 tccccgcgat tccgtcaatg agctggtgga gctgaattag cggcagtgcc cggcgcggcg
     3241 tgcttcggca gtctcggcga agacgcgcgt cgacgacgta gtagcagcac ggttcccatc
     3301 gcaaccgtcg cgacgccaac ccaaaacgcc gcctgaagtg cggtcagcgt cagctcggcg
     3361 cgcacgatca gccgccgagc ctgcccaatc ctcgagacca ccgtgcgcaa cgcgcgggtc
     3421 atcctgcgcg atcgatccat cgtccacctc tcggttgtct ccagcgtggg tgacgcatac
     3481 ccctccgccg ccgggtttag cgaggcacca cgacggctac tgctttccct tcgaacgtgt
     3541 ccgattggag agaacgagtg agcaccgacg atgcgagcgg caccacggcc aacgacctcg
     3601 gcggcgctga tgacatgctg agcccgatgg aggcaaccga ttctgacgag gtgcacaacg
     3661 acgacggcga cgaggtggtc gacccgcccg agggctggag cggggtaacc aaggtcggcg
     3721 cgtcgaccgg cgaggaccga gacggcgaga cgctcgacgg ccgacttgct caagaagtgc
     3781 ccgacgtgac cccggacgac gtcgatccgc gagacgccga tgagccgggt gaccgggacg
     3841 aggcggttct gggctccgat gccgcccccg acgatcccgg cgtccaccgc ggccagatcg
     3901 acggcgcgcc cgaggacggc gaatcgctgt tcccggtggt cgaataggtc gcagcggcgc
     3961 cctccgcgcc ggttgatcgt gaaaagcctt gctcgatctt gactttcgcg atgggcgaag
     4021 ggcgccgacc gcgtatcgcc gtcacactgg gagccagggg cgggggccat cggcttggcg
     4081 ccgtcgtata cccggcggcc gaacgccggt gttacgacgg aacaggacgg tcattgccat
     4141 gggcgcggct cagcggatcc aggtgcgaga ggcgacccaa gacggagtga ccgtcttgct
     4201 cgtcgatggc gtgctcgaca gcagcaccta cctcgagttg cgggatcgta tcgtcaaagc
     4261 cgccttggac gagactctcg cggtcatcgt caacatcagc gccttgcgag tgcctgcacc
     4321 gtcggcgtac gcagtcttca caagtgcgcg ttggcaggtc agccgttggc ccgacgttcc
     4381 tgtgctgctg gtgtgcgggc attccaccgg tcgaacagcc ctgcgccgcc agggaatcac
     4441 ccgctacgtc ccagtccacg acagtctcga cgccgccctc gccgcactcg acgacgtcgg
     4501 gaattgggtg cgccggcgtc gctccgccga attggctgcc gggcccgaat cggtcgttct
     4561 tgctggtgaa ctcgtcactg aatggctgtg ggactgggga cacagcgaca agatcgctac
     4621 cgccaaggtc atcgtcatcg aactcgtgaa gaacgtctac gcgcacacgg acagcacggc
     4681 ggagctccgg gtggaggcca acggttcctc ggtcactgtc gcggtttccg acggcagcgg
     4741 ccgattggcg agcaggatcg aaagcaccta cgagacgttc cggccgtcgg gtctgcactt
     4801 tgtggctgcg ctggcgcaca catggggatg tacgccgagc accggcggca agacggtatg
     4861 ggcgaccatc gcccgctagc cctgcctgac ttacccgttg tcgtcgtcct gttcacccgc
     4921 ggtggtgctc ggcagcgcct gcgcacccgg agtggccggg gtcgtcaaca cttcttccgt
     4981 ttcgttctcg ggctccggag atgtcacgga cggtttcgta cccggacgac ggctgtgcta
     5041 accgttaccg atgtcgagat cgtgatgacg gttccgtctt atggatgagc gaccaactag
     5101 ggtcgcaggt cctagggacc agaacggaga agaaccgatg gcgaagtatc tactgctcaa
     5161 gcattaccgg ggcgcccccg cggcggtcaa cgatgtgccg atggcccagt gggcacccga
     5221 ggaaatcgag gcgcatatca agtacatgaa cgacttcgcg gctcgtcttg agggcaccgg
     5281 agagtacgtc gacagccagg cgttggcgcc cgacggtgcg tgggtccgtt acgacggcga
     5341 aggcaggccg cccgtgactg acggcccgtt cgccgagaca aaggacctga tcgcgggctg
     5401 gatgatcctc gacgtcgaca cttaccagcg tgccgtcgag ctggccggcg agttgtccgc
     5461 ggcgcccggc gcaggcggca agccgatcca cgagtggctc gaggtgcggc cgttcctcac
     5521 cgcttcgcca accgtcaccg agtgatggcg gccgctgcgg tggacgagca gcagctgcgg
     5581 gacctgatcc caggcgtgct ggcggccctc gtccaccgcg gggcggactt cgccaccgcc
     5641 gaggacgccg tccaggaagc gttggtgcgg gccgtggaaa cgtggcccgc gcagcgtccc
     5701 gaggatccga agggctggct catcaccgcg gcgtggcggc gctttgtcga tctcacccgc
     5761 tccgacatcg ctaggcaccg ccgcgagacg cgggtgtccg accagccggc gcaaggaccg
     5821 accgctgcgg tggacgacac gctgtcgttg tatttcctgt gcgcgcaccc gagcctcact
     5881 ccggcgtcgg ccgtcgcttt gacgctgcgg gccgtcggcg gcctcaccac ccgccagatc
     5941 gcacaggcct acctcgtacc ggaagccacg atggctcaac ggatcagccg ggccaaacgc
     6001 accgtgagcg ccgtccggct gcaccgtccc ggaaatctgg gcactgtgct tcgcgtgctg
     6061 tacctcgtct tcaacgaggg gtacagcggt gacgtcgatc tggccgccga agcgatccgg
     6121 ctcgcccggc agctggcgct gatcagcgac gacccggagg ttgccgggct actcgcgtta
     6181 tttctgctgc accacgccag gcgttccgcg cgaacgcgtt cggacggcag cctggtgcca
     6241 ctggccgagc aggaccggtg cgtctggagg cgtgacttgg tcgccgaggg cgtggccgtg
     6301 ctgcagaccg cgctcgcacg cgaccggctc ggggagtacc aggcgcaggc agcgatcgcc
     6361 gccctgcacg ccgacgcgca gaccgccgcg gaaaccgact gggtgcagat cgtcgagtgg
     6421 tacgacgagc tcgtccggct caccgacagc cccgtcgtgc ggctcaaccg cgccgtcgcc
     6481 gtcggtgaag cggacggccc gcaggcgggc ctcgcagcgc tcgccgacct ggacccgacg
     6541 ctgccgcgct acaccgcctc ggccgcgtat ctccatgcgc gggccggcga caccgcgatg
     6601 gcggcagatc tctatgccgc agcggccgcg caggcgcaga acatcgccga acgcaaccac
     6661 ctcacgctac gagccgccac tctccggcaa cggctgtcgt cttcccagga cggtgagtga
     6721 cctgcagcgt tatcgccggt ggacgagtat tcgataacgt atccgggtaa tagaggaggt
     6781 acgtcccatg acgtcgaatg ccgagcgcgg ggaggtgtcc cagctcgctg cccaggttgg
     6841 ctggcagcac agggacgccg aacgcagcga ctactacctg cgccgaccgg aacgtgttca
     6901 catcgtgtgg cagggaaacc aggccatcag cgggggcgcg ttgtaccggg acaacatcct
     6961 gatgtccatc tccagggaac tggatacggt caagggctgg ttgaaccgct gatcggtccg
     7021 acatgatgct gtccgctcag ctgtggtcgg acaacgccga tgtggcttcc gaagtcctcg
     7081 ggaatccgtt cgtccgcggt atcagtgatg gttcgctgcc gcgcgaactc ttcgcaggtt
     7141 atatcgcaca ggacgccttc ttcctcgaat ccttcgcccg cgcatacgct ttggcgcttg
     7201 cccgcagctc ggacacggca acgctgctga ccctggccga tctcctcggc ggtgtccgtc
     7261 aggagctcgc actgcacgcc tcgtacgccg gctcgtgggg catcgacatg gcgggtgtca
     7321 cgccgcggcc cgcgaccctg gcctacaccg aatttctgct tgccaccgca tcgactcagg
     7381 atctgggcgt gatctacgca gccatgacgc cgtgtatgcg gctgta
//
LOCUS       DASYLX010000884         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1368326,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000884 DASYLX010000000
VERSION     DASYLX010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368326"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VGP27_20660"
     CDS             complement(<1..726)
                     /locus_tag="VGP27_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7853743.1"
                     /translation="MLLVHPVHEVLRQIPLLVGAVVVGSATGNSWWTVAALGVTIGFG
                     VLRWFTTSYRIEPDEVQMRAGVLQRKVVSVPRNRIRSVQTDARLLHQLLGLTVLRVST
                     GQHTTGDAAFALDAVRAEEVPRLRSILLADSLAPADETGGQDRPQTRLLARWHPSWLR
                     YSPLSFTGLAMILAAVGIVYQAGLGAALEHSDVADSGRDAAQRFGVITSVAVVAAVVL
                     FASVALSVLRSLVTYGNLTLRRDA"
     gene            complement(759..1259)
                     /locus_tag="VGP27_20665"
     CDS             complement(759..1259)
                     /locus_tag="VGP27_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7853744.1"
                     /translation="MSDRAGVTLVDPANPPSRKAPLVWALGAAIPWLVLVVAQVVWFV
                     LDGRLAWLHIVAAAVTVLGIAVFVVVVPLWRYRVHRWDVSPQAVYTRTGWLVQERRIA
                     PISRVQTVDTYRGPLDRLLGLANVTVTTASSAGAVRIVALDSEVADRVVAQLTDIAAI
                     GAEDAT"
     gene            1298..>1963
                     /locus_tag="VGP27_20670"
     CDS             1298..>1963
                     /locus_tag="VGP27_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV7853745.1"
                     /translation="MSQKWTSTDVPDQSGRIAIVTGANSGLGYDTAAVLADKGAHVVL
                     AVRNVDKGKEAADRIKAASPNAVVAVQELDLSSLDSVRRAADDLRAAHPRIDLLINNA
                     GVMYVPKRETTKDGFEMQFGTNHLGHFALTGLLLDNMLAVDGSRVVTVSSNGHKFWFL
                     ADIYFDDLQFERGYNRVKAYGQSKLANLMFTYDLQRRLKGKGAPTVALAAHPGFSDTE
                     LMRY"
BASE COUNT          372 a          718 c          615 g          258 t
ORIGIN      
        1 tgcatctctg cgcagcgtga gattcccgta cgtgacaagc gaccgcaaca ccgaaagcgc
       61 caccgaggcg aacagcacga ccgccgcgac gacggccacg ctggtgatga caccgaaccg
      121 ctgggccgcg tcgcgacccg agtcggcgac atccgaatgt tcaagcgctg caccgaggcc
      181 cgcttgatag acgatgccca ccgcggcgag gatcatggcc agaccggtga agctgagcgg
      241 gctgtagcgc agccacgagg gatgccagcg agccagcagc cgggtctgcg gccggtcctg
      301 gccgccggtc tcgtcggcag gcgccagcga gtcggccagc aggatcgagc gcagccgcgg
      361 cacctcttcg gcgcgcacgg cgtccaacgc gaacgccgcg tcccccgtgg tgtgctggcc
      421 ggtgctgacc cgcaataccg tcaacccgag cagctggtgc agcagccggg cgtcggtctg
      481 caccgaacga atccggttgc gcggcaccga aaccaccttg cgctgcagga caccggcgcg
      541 catctgcacc tcgtcgggct cgatgcgata gctggtggtg aaccagcgca gcacaccgaa
      601 accgatcgtc acccccaacg ctgccaccgt ccaccaggag ttgccggtcg ccgaacccac
      661 cacgaccgcg cccaccaaca acggaatctg ccgcaacacc tcatgcaccg ggtgcaccaa
      721 caacattcgc gggctgagcc ggtgccacac ccggtccgtc acgtggcgtc ctccgcgccg
      781 atggcggcga tgtcggtgag ctgcgcgacg acccggtcgg ccacctcgga gtcgagcgcg
      841 acgatgcgca ccgcaccggc cgacgacgcg gtcgtgacgg tgacgttggc cagcccgagc
      901 agccgatcca acggtccgcg ataggtgtcg acggtctgca cccgagaaat cggcgcgatc
      961 ctgcgctcct gcaccagcca gcccgtcctg gtgtacaccg cctgcgggct gacgtcccag
     1021 cggtgcacgc ggtaccgcca cagcggcacg acgaccacga acaccgcgat gcccaacact
     1081 gtgacggcgg ccgcgacgat gtgcagccag gccagccggc cgtcgagcac gaaccacacc
     1141 acctgggcga ccaccaacac cagccacggg atggccgccc cgagcgccca caccaggggc
     1201 gccttgcggc tcggcgggtt ggcgggatca accaaggtca cccctgcgcg atccgacatg
     1261 ggaacgacgg taaccgtccc tgggtaatgt gctgatcatg agccagaagt ggacctccac
     1321 cgatgtaccc gaccagtccg gccgcatcgc catcgtcacc ggcgccaact ccggtctcgg
     1381 ctacgacacc gccgccgtcc tggcggacaa gggcgcgcat gtcgtgcttg ccgtgcgcaa
     1441 cgtcgacaag ggcaaggagg ccgccgaccg gatcaaggcc gccagcccga acgccgtggt
     1501 agccgtgcag gaactggatc tgtcgtcgct cgacagcgtc cgcagggccg ccgacgacct
     1561 ccgcgccgcc cacccgcgca tcgacctgct gatcaacaac gcgggcgtga tgtacgtgcc
     1621 gaagcgcgag accactaagg acggcttcga gatgcagttc ggcaccaacc acctcggcca
     1681 cttcgcgctg accggcctgc tgctggacaa catgctggcg gtcgacgggt cgcgggtggt
     1741 cacagtcagc agcaatggac acaaattctg gttcctcgcc gatatctact tcgacgactt
     1801 gcagttcgaa cgcggttaca accgggtcaa ggcctatggc cagtccaaac tggccaacct
     1861 gatgttcacc tacgacctgc agcggcgcct gaagggcaaa ggcgcgccga ccgtcgcact
     1921 cgcggcccat cccggcttct ccgataccga gctcatgcgc tac
//
LOCUS       DASYLX010000885         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_490418,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000885 DASYLX010000000
VERSION     DASYLX010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490418"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGP27_20675"
     CDS             complement(<1..271)
                     /locus_tag="VGP27_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7853746.1"
                     /translation="MKAPARAVRRLRRPVRREQMSDEVAGRLRAAIMSGTLLPGTFIR
                     LDETAAALGVSITPVREALRTLRGDGMVLLEPHRGHVVAPLSRRDI"
     gene            374..2023
                     /gene="fadD5"
                     /locus_tag="VGP27_20680"
     CDS             374..2023
                     /gene="fadD5"
                     /locus_tag="VGP27_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD5"
                     /protein_id="HEV7853747.1"
                     /translation="MPLTAPLAPTAQPYLARRQNWTNQLARHAHMQPDATALRHLGRT
                     TTWAELDRRVSALAGALSRRGVGFGDRVLILMLNRTEFIESFLAANLLGAIAVPVNFR
                     MTPPEIAFLVSDCDATVMIAESVLAKVATAVRDLEPKLETVVVAGGAADDGLLGYDDL
                     LVEEGEPAPPVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLAGQALTFLFTNGVDINN
                     DVGFIGVPLFHIAGIGNMITGLLLGRPTVVYPLGDFDPGALLDVLEAEKVTGIFLVPA
                     QWQAVCAMQQASPRRLRLRVLSWGAAPASDTLLRSMSETFPGTQILAAFGQTEMSPVT
                     CMLLGDDAIRKLGSVGQVIPTVDARVVDDDMNDVPIGSVGEIVYRAPTLMEGYWNNPT
                     ATAEAFAGGWFHSGDLVRRDEEGYIWVVDRKKDMIISGGENIYCAEVENVLAAHPAIV
                     EVAVIGRSDEKWGEVPVAVVAVRAVSDSSLKLVDLNEFLTQRLARYKHPKHLEVVDAL
                     PRNPAGKVLKTELRSRFAAERSVDADERVSAARVSAGKAKG"
     gene            2237..3037
                     /locus_tag="VGP27_20685"
     CDS             2237..3037
                     /locus_tag="VGP27_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853748.1"
                     /translation="MTATRSGIGDYVSKQTRPALEAVGGFVRMCVLTGRALFRRDFQY
                     REFVLQSWFLFKVSFLPTLAVSIPLTVLLIFTLNILLTEFGAADISGAGAALAAVTQL
                     GPLVTVLVVAGAGSTAICADLGARTIREEIDALEVLGIDPIHRLVVPRVVASTFVALL
                     LNGAVITIGLVGGFIFGVYLQNVSAGAYVSTLTLVTGLPEVLISVIKATTFGLIAGLV
                     GCYRGLTVAGGAKGVGTAVNETLVLCVIALFAVNVVLTTIGVRFGTGS"
     gene            3039..>3827
                     /locus_tag="VGP27_20690"
     CDS             3039..>3827
                     /locus_tag="VGP27_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853749.1"
                     /translation="MSTTNVLKARFPRGYARTERLAGAPARFLDSVGHVAWFVVTAIG
                     SIGHALRYYRKETLRLIAEIGMGTGAMAVVGGTVAIVGFVTLSGSSLVAIQGFASLGN
                     IGVEAFTGFFAAMINVRIAAPVVAGQALAATVGAGATAELGAMRISEEIDALEVMGIK
                     SISYLVSTRVMAGFVVIIPLYAMAIIMSFLSAQITTTFFYGQSIGTYEHYFRTFLRPD
                     DVFWSFVQAIIISVIVMLNHCYYGYFASGGPVGVGEAVGRSMRAS"
BASE COUNT          596 a         1253 c         1271 g          707 t
ORIGIN      
        1 cgatatcgcg cctgctcaag ggggccacga cgtggccgcg gtggggctcc agcagcacca
       61 tgccgtcacc acgcagggtg cgtagcgctt cgcgcaccgg ggtgatgctg acgcccagcg
      121 cagcggcggt ttcgtcgagc cggatgaagg tgcccggcaa cagcgtcccg gacatgatcg
      181 cggcccgcag acgcccggcg acctcgtctg acatctgttc ccgccgcacg ggccgacgca
      241 ggcgtcgaac ggctctcgcc ggtgccttca tcggcctcca ttccaggggt tgtcccttga
      301 ccgcggaggc catagtgtga ccgaggcaac cgcttgtttg atcaaatata aaacgccgtg
      361 caaccaggag ctcctgccgt tgaccgctcc actcgcgccg accgctcagc cttatctggc
      421 gagaaggcag aactggacca accagctggc gcggcacgcg cacatgcagc ccgacgcgac
      481 ggctctgcgc catctcggcc gcaccaccac ctgggctgaa ctcgaccgcc gcgtcagcgc
      541 gctggcgggt gcgctcagcc gtcgcggcgt cggtttcggc gacagggtgc tgatcctgat
      601 gcttaaccgg accgagttca tcgagtcctt cctggccgcc aacttgctcg gcgccatcgc
      661 ggtcccggtc aacttccgca tgacgccacc cgaaatcgcc ttcctggtca gcgactgcga
      721 cgccacggtg atgatcgccg agtcggtgct ggcgaaggtg gcgacggcgg tcagggatct
      781 cgaaccgaag ctcgaaacgg tggtcgtcgc gggcggggcc gccgacgacg gtctgctggg
      841 ctacgacgac ctgctggtcg aagagggtga gcccgcgccg ccggtcgaca tccccaacga
      901 ctcccccgcg ctgatcatgt acacctcggg caccacgggg cggcccaagg gcgcggtgct
      961 cacccacacc aacctggcgg ggcaggcgct gaccttcctg ttcaccaacg gcgtcgatat
     1021 caacaacgac gtcggcttca tcggcgtccc gctgttccac atcgcgggca tcggcaacat
     1081 gatcaccggt ctgctgctcg gccggccgac ggtggtgtat ccgctcggcg atttcgaccc
     1141 cggcgcgctg ctcgacgtgc tggaggccga gaaggtcacc ggcatcttcc tcgtacctgc
     1201 gcagtggcag gcagtttgcg ctatgcagca ggccagtccg cgtcggctgc ggctgcgcgt
     1261 gctgtcgtgg ggcgcggcgc ccgcatcgga cacgctgttg cgcagcatgt ccgagacctt
     1321 tcccggcacc cagattctcg cggcgttcgg acagacggag atgtcgccgg tgacgtgcat
     1381 gttgttgggg gacgacgcaa ttcgcaaact cggctcggtc ggacaggtca ttccgacggt
     1441 tgacgctcgc gtcgtcgacg acgatatgaa cgacgtaccg atcggttcgg tcggcgaaat
     1501 cgtctaccgc gccccgacgc tgatggaggg ctactggaac aacccgaccg cgaccgcgga
     1561 ggcgtttgcc ggtggctggt tccactccgg cgacctggtg cgccgggacg aagagggcta
     1621 catctgggtg gtcgaccgca agaaggacat gatcatctcc ggcggagaga acatctattg
     1681 cgcggaggtg gagaacgtcc tcgccgccca tccggcgatc gtcgaggtcg ccgtgatcgg
     1741 gcggagcgat gaaaagtggg gcgaagtccc cgtcgccgtt gtggcagtgc gggcggtgtc
     1801 cgattcctct ctcaaactgg tcgatctgaa cgaattcctc acgcagcggc tcgcccggta
     1861 caagcatccg aagcatctgg aggtcgtcga cgccctcccg cgcaaccccg caggcaaagt
     1921 cctcaaaacg gaactgagaa gtcggttcgc cgcggagaga tcggttgacg ccgacgaaag
     1981 ggtctctgct gcaagggttt ctgcaggtaa ggccaagggt taaagagggc gaggtggggt
     2041 tgctaacggt tataggctca atccgacgga tgcggtgcaa ccctccgggg gcatcgggta
     2101 cagtcctgtg gtccgtctta ctacccgcgg gtagggagac ggcgatcaca ggatcgggtt
     2161 cggtgcaagg agggggcgtg cgacaggtgc tgccgtaccg ccacgtcggc cgaagcgtcg
     2221 gcgcgggagt gcgctgatga ctgcgacacg atctgggatc ggcgactacg tctccaagca
     2281 gacccggccg gcgctggagg ccgtcggcgg cttcgtgcgc atgtgcgtgc tgaccggaag
     2341 ggcgctgttc cggcgggatt ttcaataccg tgagttcgtc ctgcagagct ggttcctgtt
     2401 caaggtctcc ttcctgccca cgctcgctgt ttccatacct ctgaccgtcc tgctcatctt
     2461 caccctcaac atcctcctca cggagttcgg cgccgccgac atctcaggcg ccggcgcggc
     2521 gctggccgcc gtcactcagt tggggcccct ggtgaccgtg ctcgtcgtcg ccggtgccgg
     2581 ctcgacggcg atctgcgctg acctcggtgc ccgcaccatc cgcgaggaga tcgacgcgct
     2641 cgaggtcctc ggcatcgatc cgatccatcg actcgtggtg cctcgcgtgg tcgcatcgac
     2701 attcgtcgcc ctactgctca atggtgcggt gatcaccatc ggtctggtcg gcggcttcat
     2761 cttcggcgtt tacctgcaga acgtgtccgc gggcgcctac gtctccacgt tgacgctcgt
     2821 caccggattg cccgaggtgt tgatctccgt catcaaggcc accaccttcg gcttgatcgc
     2881 cgggctggtc ggctgctacc gcggcctgac cgtcgccggt ggcgcgaagg gcgtcggcac
     2941 cgcggtgaac gagacgctgg tgctctgcgt catcgccctg ttcgcggtga acgtcgtgct
     3001 gaccacgatc ggtgttcggt tcggcacggg gagctgacat gagcacaacg aatgtcctca
     3061 aggcccgctt cccccgggga tacgcgcgca ccgagcggtt ggccggtgcg cctgcgcggt
     3121 tcctcgacag cgtggggcat gtcgcctggt tcgtcgtgac cgcgatcgga tcgatcggac
     3181 acgcgctgcg ctactaccgc aaggagacgc tgcggctgat cgccgagatc ggcatgggca
     3241 ccggcgcgat ggcggtggtc ggcggcacgg tcgccatcgt cgggttcgtc accctgtccg
     3301 ggtcctcgct ggtcgctatc caagggtttg cctcgctggg caacatcggt gtcgaggcgt
     3361 tcaccgggtt cttcgccgcg atgatcaacg tgcgcatcgc cgcacctgtc gttgcgggtc
     3421 aggccctggc cgccaccgtc ggcgcgggcg ccacggcgga actcggcgcc atgcggatca
     3481 gtgaggagat cgacgcgctg gaggtgatgg gcatcaagtc catctcctat ctggtgtcga
     3541 ccagggtgat ggcgggcttc gtggtcatca tcccgctcta cgcgatggcg atcatcatgt
     3601 cgtttttgtc cgcgcagatc acgacgacgt tcttctacgg ccagtcgatc ggaacctacg
     3661 agcactactt ccggacgttc ctgcggccgg acgacgtctt ctggtccttc gtgcaggcga
     3721 tcatcatctc ggtgatcgtc atgctcaacc actgctatta cggctacttc gccagcggcg
     3781 gaccggtcgg cgtcggcgag gccgtcggcc ggtcgatgcg cgcctcg
//
LOCUS       DASYLX010000886         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3233859,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000886 DASYLX010000000
VERSION     DASYLX010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233859"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGP27_20695"
     CDS             <1..556
                     /locus_tag="VGP27_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV7853750.1"
                     /translation="FVQSTDDAVRFLTDQHNLIKDLFEEVFAASSDEGRDKAFTDLRQ
                     LLAVHETAEEMVVHPRARNELSNGDAVVDARLAEEHEAKEQLSKLEKMDVGSKEFLTE
                     LTKFRDAVIEHAEHEETEEFSKLKRKLDADDLSRMAKALQAAEAIAPTRPHPGVESAK
                     MNFAVGPFASMLDRARDLIKQAVS"
     gene            799..1536
                     /gene="sepIVA"
                     /locus_tag="VGP27_20700"
     CDS             799..1536
                     /gene="sepIVA"
                     /locus_tag="VGP27_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepIVA"
                     /protein_id="HEV7853751.1"
                     /translation="MYRVFEALDELGAIVEEARGVPMTAGCVVPRGDVLELIDDIKDA
                     IPGELDDAQDVLDARDQLLNEAKDHSGSMVATANAEADSMINHARAEADRLLADAKSQ
                     ADRMVAEARQHSERMVGEAREEAARVSATAKREYEASAGRAKAEADRLIESGNLSYEK
                     AVQEGIKEQQRLVSQTETVQAANAEAARLIDTAHAEADRLRGECDIYVDSKLAEFEEF
                     LNGTIRSVGRGRHQLRTAAGTHDYVTR"
     gene            1577..2131
                     /locus_tag="VGP27_20705"
     CDS             1577..2131
                     /locus_tag="VGP27_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV7853752.1"
                     /translation="MATQRRSRSPLVINIARLGRRPGSMMTVQETVPSPMRIGVELVA
                     IDEGAPLELDLRIQSVSEGALVTGTMSAPTTGECARCLTPITGHVEIDLTELFAYPDS
                     TTEATTEADEVGHVVDNSVDLEQPIVDAVGLALPFSPLCGPDCQGLCPDCGIPMSTAE
                     PGHRHETIDPRWAKLASMLENDDK"
     gene            2128..>2517
                     /locus_tag="VGP27_20710"
     CDS             2128..>2517
                     /locus_tag="VGP27_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEV7853753.1"
                     /translation="MTGERAALVEALGVELPDDLLTMALTHRSYSYENGGLPTNERLE
                     FLGDAVLGLTITEELYHRHPDCSEGDLAKLRASIVNTQALADVGRTLSDGGLGAHLLL
                     GKGEQNSGGAGKASILADGVESLLGAIY"
BASE COUNT          502 a          809 c          835 g          371 t
ORIGIN      
        1 attcgtccag tcaaccgatg acgcagtcag attcctcacc gatcagcaca acctgatcaa
       61 ggatctgttc gaagaagtgt tcgccgcatc cagcgacgag ggacgcgaca aggcgttcac
      121 cgaccttcgc cagctgctgg ccgtgcatga gaccgccgag gagatggtgg tgcacccgcg
      181 cgcgcggaac gaactcagca atggcgacgc cgtcgtcgac gcccggctcg ccgaagagca
      241 cgaggccaag gagcagttgt cgaagctcga gaaaatggac gtcggctcca aagagttcct
      301 caccgaactc accaagttcc gcgatgccgt gatcgagcac gccgaacacg aggaaaccga
      361 ggaattcagc aaattgaagc ggaagctcga cgctgacgac ttaagtcgga tggcgaaagc
      421 actgcaagcc gccgaggcca tcgcgccgac ccgtccgcac ccaggtgtgg agtcggcgaa
      481 gatgaatttc gcggtgggcc cattcgcctc gatgctcgat cgggctcgcg acctgataaa
      541 gcaggccgtt tcctagcgaa ttcgttgagc aggcaagacc gcccaggcgc ttgaagtccg
      601 cgggcggtct tggtcattgc ccgatatgcc atttgtgcgg ccattgacat aatgttgccg
      661 atacgaagtc agccgatagt tgaccggcct ggggtggggc agctggaagc cgaccggcga
      721 cacaccggcg gcgctagcat agtcacaggg gtaacaccag gcacactggt aacaacaact
      781 acgcctggag ggtgtgccgt gtaccgagta tttgaagcgc ttgatgagtt gggcgcgatc
      841 gtcgaagagg cccgcggcgt cccgatgacg gcaggttgtg tggtgccccg cggcgatgtg
      901 ctcgagttga tcgacgacat caaggacgcg atccccggcg aactcgacga cgcgcaggac
      961 gtgctggacg cgcgtgacca actgctcaac gaggccaagg accattccgg ttcgatggtg
     1021 gcgacggcca acgccgaggc cgactcgatg atcaaccacg cgcgtgccga ggccgatcgg
     1081 ctgctggccg acgccaagtc acaggctgac cggatggtcg ccgaggcccg ccagcacagc
     1141 gagcgaatgg tcggggaggc ccgtgaggaa gccgcccgcg tcagcgcgac ggccaagcgc
     1201 gaatacgagg cgagcgcggg ccgggcgaag gccgaggcgg accgcctgat cgagagcggc
     1261 aacctctcct acgagaaggc cgtccaggag ggcatcaagg agcagcaacg cctggtgtcg
     1321 cagaccgaaa ccgtccaggc ggcaaacgcc gaggccgccc gtctgatcga cacggcccat
     1381 gccgaggccg accggctgcg cggcgaatgc gacatctacg tcgacagcaa gctcgcggag
     1441 ttcgaggagt tcctcaacgg aacgatccgc tcggtgggcc gcgggcgcca ccagttgcgc
     1501 accgccgcag gcacccacga ctacgtgacc cgctagccgc gctcacccgt cgagggcgtc
     1561 gccgtaccat cgaagtatgg cgacgcaacg tagatcccgc tcgccgctcg tcatcaacat
     1621 cgctcggctc ggccggcggc cggggtcgat gatgaccgtt caggaaacgg tgcccagccc
     1681 gatgcgcatc ggcgtcgagt tggtggcgat cgacgaaggc gcgcccctcg aactggacct
     1741 gcggatccag tcggtatccg aaggtgccct cgtcaccgga acgatgtcgg cgccgaccac
     1801 cggcgagtgc gcccgctgcc tgacaccgat caccggacac gtcgaaatcg acctcaccga
     1861 gctgttcgcc tatcccgaca gcaccacgga ggcgaccacg gaggccgacg aggtcggcca
     1921 cgtcgtcgac aattccgtcg acctcgagca gccgatcgtc gatgcggtgg ggctggcgtt
     1981 gccgttctcg ccgctgtgcg ggccggattg ccaagggctg tgccctgact gcggcatccc
     2041 gatgtcgacc gcagagcccg ggcatcgtca cgagacgatc gatccgcgct gggcgaagct
     2101 cgcgtcgatg ctggagaacg atgacaagtg acgggtgagc gcgccgcact tgtcgaggcc
     2161 ctcggagtcg aactgcccga cgacctgctc acgatggcgc tgacacaccg cagctactcc
     2221 tacgagaacg gcgggctgcc gaccaacgag cggctggagt ttctcggcga tgcggttctc
     2281 gggctgacca tcaccgagga gctctaccac cggcatcccg attgctcgga gggcgatctg
     2341 gccaagctgc gcgcgagcat cgtcaacacc caggcgctgg ccgatgtcgg ccgcacgctg
     2401 tccgacggcg gacttggcgc ccacctgtta ctgggcaagg gcgagcagaa ctccggcggc
     2461 gcgggcaagg cgagcatttt ggccgacgga gtcgaatcgc tgctcggcgc aatctat
//
LOCUS       DASYLX010000887         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2905820,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000887 DASYLX010000000
VERSION     DASYLX010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905820"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGP27_20715"
     CDS             <1..361
                     /locus_tag="VGP27_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7853754.1"
                     /translation="DLGEWVQADPRAGRPLFDQLRSQIIDGIRNGRLPPGTRLPTVRE
                     LAAELSMAVNTVARAYRELESAGMLETRGRFGTFVARTDPADAAMATAAHAYATAAKA
                     LGIPKGEALKYVEAAFG"
     gene            complement(364..1188)
                     /locus_tag="VGP27_20720"
     CDS             complement(364..1188)
                     /locus_tag="VGP27_20720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853755.1"
                     /translation="MSKADGSALTGVSETALLTLLARATEARRPDSLIDDPMAIKLVD
                     EIDFDGAKFGFARRQDMAPRALTFDKYTRRYLLDHPSATVVALAEGLQTSFYRLDASG
                     IGDQFRWLTVDLPPIIELRDQLLPRSDRVSMVAQSALDFSWMDRVDAADGVFITTEGL
                     LMYLQPDEALGLIAECAARFPGGQLMFDLPPMLFASWIRGGSRTSLRYRVPPMPFTLS
                     PSEVANLVNTVPGVRAVHDLPMVPGRGKVLNSLMWTLQRVALLDSVRPIVALLEFG"
     gene            complement(1185..1706)
                     /locus_tag="VGP27_20725"
     CDS             complement(1185..1706)
                     /locus_tag="VGP27_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV7853756.1"
                     /translation="MTRYAAFLRGVNVGGVNLKMAEVAAALENAGFTNVKTILASGNV
                     LLDSRSGVDTVRKNAEKALRDGFGYDAWVLAYDIDTVKAISDKYPFEREVEGRHSYVT
                     FVTDPDVLDELAALAKDAGADEKIKRGKGVIYWQVPNKGTLDTTIGKTMGKKRYKSST
                     TTRNLRTIDKVLK"
     gene            complement(1703..2527)
                     /locus_tag="VGP27_20730"
     CDS             complement(1703..2527)
                     /locus_tag="VGP27_20730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853757.1"
                     /translation="MAVISGDTLEGVSATSLWTLHNRGTEAKRSDGVIRDPWAVALLD
                     SIAYDYLKFGKPNQSHGLRAAAFDGVMRDYLTAHPRAAVVALADGLQTSFWRLQAAGC
                     TDELTWYSIDLPPVMALRAQLLPHDDRIVALGQSALDHGWMDRVDDSHGVFITAEGLL
                     MYLQPHDAIALIRECAARFPGGRMMFDSIPPFFSRRTLRGVKLSDRYTAPRMPFGMSP
                     DDALALADTLPGVRSARDIMLPPGRGLWKVAAWPPLDRIGLMRRIRPSITLLEFDR"
     gene            2587..3030
                     /locus_tag="VGP27_20735"
     CDS             2587..3030
                     /locus_tag="VGP27_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7853758.1"
                     /translation="MARQVFDDKLLAVISNNTLGVLATIKRDGRPQLSNISYHFDPRN
                     LAIQASITEPRAKTRNLRRDPRASLHVSSDDGWSYAVAEGNAILTPPAASPHDDTVEA
                     LIALYRNIAGEHPDWDDYRRAMVDDRRVMMTLPISHLYGMPPGMR"
     gene            3083..3250
                     /locus_tag="VGP27_20740"
     CDS             3083..3250
                     /locus_tag="VGP27_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HEV7853759.1"
                     /translation="MAEAPEDDNKKKFREALERKNAKSGGGAAHKDGGNKQPKAHGPL
                     ESRREFRRKSG"
     gene            complement(3289..>4043)
                     /locus_tag="VGP27_20745"
     CDS             complement(3289..>4043)
                     /locus_tag="VGP27_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV7853760.1"
                     /translation="GYLNLVAWLIPGMLGVAYRRHLLAGRAALMLAAVMFVVNLSLMW
                     VGPYELSLVGIETQQLKNMTPPSLLLASHAIILCALAIAAAPAIARWAKRPRVWWLAA
                     IGNSGAMTLYLWHIPALLAMHLVFDYLGHPRYDPSAAGFTALSILQLVIMACLVATVF
                     VALRPLEKNPLPLWDGGFVSAPGSRSAAVGALLCVAGAATLASVGWGLKGQGLYCVAV
                     MLAALIAARSLAGDRPVTTQRACAASLPASTR"
BASE COUNT          735 a         1344 c         1354 g          610 t
ORIGIN      
        1 cgatttgggg gaatgggttc aggctgatcc gcgggccggc aggccgttgt tcgaccagct
       61 caggagtcag atcatcgacg ggatccgaaa tggccggctg ccaccgggca cgcggctgcc
      121 gacggtgcgc gagctcgcgg ccgaactgag catggcggtc aacaccgtgg cccgcgcgta
      181 tcgcgagctg gaatcggcgg gaatgctcga aacacgcggt cgcttcggca cattcgtcgc
      241 ccgcaccgat ccggccgatg cggcgatggc cactgccgca cacgcctacg cgacggccgc
      301 caaggcgctc ggcatcccga agggtgaggc gctcaagtac gttgaggcgg cgttcggcta
      361 gtgctagccg aactccagca aggcgacgat cggccggacc gagtcgagca gcgcaacccg
      421 ctgcaatgtc cacatcaacg aattgagcac tttgccgcgg cccggcacca ttggcagatc
      481 atgcacggcc cggacgccgg gcacggtgtt gaccaggttt gcgacctcag aaggcgacag
      541 cgtgaacggc atcggcggca cccggtagcg cagcgatgtc cgtgaaccgc cccttatcca
      601 cgaagcgaaa agcatcggcg gcaagtcgaa catcagctgc ccgccgggga atcgcgcggc
      661 gcattcggcg atcaggccca gtgcctcgtc gggctgcaga tacatcagca gaccctcggt
      721 cgtgatgaat acgccatcgg cggcatccac ccgatccatc cagctgaagt ccagagcgga
      781 ctgcgcgacc atcgagaccc ggtccgagcg cggcaacagc tggtcgcgca gctcgatgat
      841 cggcggcaaa tcgacggtca accagcggaa ttggtcgccg ataccggatg cgtccagccg
      901 atagaagctg gtctgcagcc cctcggccag cgccacgacg gtcgccgatg ggtggtcgag
      961 caggtatcga cgggtgtact tgtcgaaggt gagcgcccgc ggagccatgt cctggcggcg
     1021 ggcgaagccg aacttcgctc cgtcgaagtc gatctcgtcg acgagcttga tcgccatcgg
     1081 gtcgtcgatc aacgagtccg gcctgcgcgc ttcggtagcc cgtgccagca gcgtgagcag
     1141 cgccgtctcg gagaccccgg tcagcgcgga gccgtctgcc ttgctcattt cagaaccttg
     1201 tcgatcgtgc gcaggttgcg tgtggtcgtc gacgacttgt agcgcttctt gcccatagtc
     1261 ttgccgatcg tggtgtccag ggtgcccttg ttggggacct gccagtagat gacgcccttg
     1321 ccgcgcttga tcttctcgtc cgcaccggcg tccttggcga gtgcggccag ttcgtcgagg
     1381 acgtcggggt cggtgacgaa ggtgacgtag ctgtggcgcc cctcgacttc gcgttcgaac
     1441 gggtacttgt cggatatcgc cttcacggtg tcgatgtcgt aggcaagcac ccaggcgtcg
     1501 tagccgaatc cgtcgcgcaa cgccttctcc gcgttcttgc gtacggtgtc gacaccagat
     1561 ctgctgtcca acaacacatt accgctcgcc aggatggtct tgacgttcgt gaagccggca
     1621 ttctccagcg cggcggccac ctcggccatc ttcaagttga ccccgccgac gttcactccg
     1681 cgcaggaacg ctgcgtatcg ggtcaccggt cgaactccag cagcgtgatg ctgggccgga
     1741 tgcggcgcat gaggccgatc cggtccagcg gcggccaggc ggccaccttc cacagtccgc
     1801 gaccgggcgg cagcatgatg tcgcgcgcag agcggacgcc gggaagcgtg tcggccagcg
     1861 caagggcgtc gtcgggtgac atcccgaacg gcattcgcgg cgccgtatag cggtccgaga
     1921 gcttgacgcc tctcaacgtt cggcggctga agaacggcgg gatgctgtcg aacatcatcc
     1981 ggccgccggg aaagcgagcg gcgcattcgc ggatcagcgc gatggcgtcg tggggttgca
     2041 ggtacatcag cagaccctcg gcggtgatga agaccccgtg cgagtcgtcc acccggtcca
     2101 tccagccgtg atcgagcgcc gactggccga gagcgacgat gcggtcgtcg tgcggaagca
     2161 attgcgcgcg cagtgccatc accggcggca gatcgatgga ataccaggtc aattcgtcgg
     2221 tgcagcctgc ggcctgcaga cgccagaaac tggtctgcaa cccgtcggcc agcgccacca
     2281 ccgccgccct cggatgtgcg gtcagatagt cgcgcatcac cccgtcgaac gccgccgccc
     2341 gcaggccgtg cgactggttg ggcttgccga acttcaggta gtcgtaggcg atcgagtcca
     2401 gcaacgcgac ggcccacggg tcacggatca ctccgtcgga ccgcttcgcc tcggtgccgc
     2461 ggttgtgcag ggtccacagc gaggtcgccg agacgccttc cagggtgtca ccgctgatta
     2521 ccgccattcg ctcatagtag gttcgaacag cggtggcccc ggctgtggcc gacgtaggct
     2581 caaggtatgg cacgccaggt cttcgacgac aagctgctcg cggtgatcag caacaacacg
     2641 ctcggcgtgc tggcaaccat caagcgggat ggcaggccgc agctgtccaa catctcctac
     2701 cacttcgacc cccggaacct cgccatccag gcgtcgatca ccgaaccgcg ggccaagacc
     2761 cgcaacctgc gccgtgaccc ccgggcgtcg ctgcatgtca gcagtgacga cggctggtcg
     2821 tatgcggtcg ccgaggggaa cgcgatcctg actccgcccg ccgcatcccc gcacgacgac
     2881 accgtcgagg cgctgatcgc cctgtaccgc aacatcgccg gtgagcatcc cgactgggac
     2941 gactaccggc gggccatggt cgacgaccgc agggtcatga tgacgctgcc gatctcgcac
     3001 ctgtacggca tgccgcccgg catgcgctga ggccgggcaa gaggcagcga cggcgccgga
     3061 cggctcgcgc taagctgcgg ctatggctga agcaccggaa gacgacaaca agaagaagtt
     3121 ccgggaagcg ctggagcgca agaacgcgaa gtcgggcggc ggtgccgctc acaaggacgg
     3181 cgggaacaag caaccgaagg cgcacggacc gctcgagagc cggcgggagt tccgtcgcaa
     3241 gagcggttag ggaaccgcac gcgaggagcg gcgcggcttc ggcgccacct acctcgtgct
     3301 ggctggcagg ctggccgcgc aggcccgctg agtggtgacg ggccggtcac cggccaacga
     3361 tctggccgcg atcagcgccg ccagcatcac cgcgacgcag tagagcccct ggcccttgag
     3421 gccccaaccg accgacgcca gcgtcgccgc acccgccacg cacagcaggg cacccaccgc
     3481 cgcactgcgt gaaccggggg cggacacgaa gccgccgtcc cacagcggca gcgggttctt
     3541 ctccaacggc cgcagcgcga cgaacaccgt cgccacgagg caggccatga tcaccagttg
     3601 caggatgctg agcgcggtga agccggcagc gctcgggtcg tagcgcggat ggcccaggta
     3661 gtcgaagacc aggtgcatgg ctagcagtgc cgggatgtgc cacaggtaga gcgtcatcgc
     3721 cccggagttg ccgatggcgg ccagccacca cacccgtggc cgcttcgccc accgcgcgat
     3781 cgcgggcgcg gccgcaatag ccaacgcgca caggatgatc gcgtgactcg ccaacagcag
     3841 cgacggcggt gtcatgttct tcagctgttg ggtctcgata ccgacgaggc tgagttcgta
     3901 gggccccacc cacatcagcg acaggttgac gacgaacatg accgcggcga gcatcagggc
     3961 cgcgcggcct gcgagaagat gccgacggta ggcgacgccg agcatgccgg ggatcaacca
     4021 ggcgaccagg ttgagatagc cca
//
LOCUS       DASYLX010000888         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2685700,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000888 DASYLX010000000
VERSION     DASYLX010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685700"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1677)
                     /locus_tag="VGP27_20750"
     CDS             complement(535..1677)
                     /locus_tag="VGP27_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV7853761.1"
                     /translation="MRSRAAPDGRCLDALGAVGALAHHAELADRFEQRSQARTDGSSV
                     VDQQPGSPPRRRWWSQGASSRRDGATSTAVLRVLERLLARSLVGHGISCRVAAPSRLQ
                     RPSGDRVKTDRRDARHLARLLHLDEIVSVTVPSVGQEAARDLVRAREDVRRDLLAARH
                     RLSKLLLRQGIVYYGGKPWTGNHDRWLQARRRLAVFDQPGLGLAFDIAYDTVLATLAR
                     RDRLDAAIAQMATSSEYAPVVTRLGCLRGVSTLTAFGLAVEIGDWQRLTGRTIGAYLG
                     LVPTESSSGGSRSQGSITKTGNAHARRLLIEAAWHHRRPDRPSVTLRRRQDLASPAAR
                     DRGQRANRRLRQRWARFDARHKLPVVANTAIARELAGWCWSLAVLD"
     gene            1677..1910
                     /locus_tag="VGP27_20755"
     CDS             1677..1910
                     /locus_tag="VGP27_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853762.1"
                     /translation="MDAAVPGIDGIAATSRLLPSRSRFPGPVPDILGGGRHDPSGVRR
                     RCRRLPVEVGHEFGLPYEHVCDLSQDLPEIVLN"
     gene            complement(2006..2419)
                     /locus_tag="VGP27_20760"
     CDS             complement(2006..2419)
                     /locus_tag="VGP27_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV7853763.1"
                     /translation="MTAPVAGLLDTSTVINLPRLTAATALPDVPLISTITLAELSVGP
                     LVATSERDRADRQAVLQQAEADFDPLPFDAAAARAFGRVAAALRRSGRKPQARAYDAM
                     IAAVALARGLPVHTSNPDDFAGIDGLTVVPVPLPG"
     gene            complement(2416..2652)
                     /locus_tag="VGP27_20765"
     CDS             complement(2416..2652)
                     /locus_tag="VGP27_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEV7853764.1"
                     /translation="MDEVTIRDLRNHGGEVMDRVEHGETLTVTRSGVPVGELRPLPRP
                     RLTASTLLARWRSVPAVDTQAFRVDVDAVLDPSL"
BASE COUNT          405 a          932 c          989 g          415 t
ORIGIN      
        1 ctgggtcacc gtcctggacg agcacgagcg ccggctgaac gtggtcggct ccgatcacct
       61 ggccgacgga gctcacgtgg tcgccgtcga cgacaccacg caccacagct actacccgct
      121 acccgccggc gcggacggcc atcccgcgct cctggaggca gcacccactg cctaaaaggg
      181 cgtgtcctgt aagggttctg acctgcggtc ggtgattatg ctcgggtggt agggcgtggt
      241 gagctgacgg acaaggcctg ggcgcgggca ccgcttcgtg tccggatggc gggctcgggt
      301 ggtggcgagc gtgtcggcgt tcgacgcgtt gtcagtctga tattcgcggg ttggttaccg
      361 caggacggac tgttcggctc gagcaggatc cgcggtctag cagcgagcgt cacctgggtt
      421 ccgctcccca gcgatgggcg ggcggaaggg gttgctcata gggcgttcgc gggtccaccc
      481 acgggtgcag ccgtcaccac cgcgtcccgg tccggacgat aaggagccgc cgcctcagtc
      541 gaggacggcg agtgaccagc accagccggc gagctcgcgg gcgatcgcgg tgttcgctac
      601 gaccgggagt ttgtggcggg cgtcgaaccg ggcccaacgc tggcgcagac gacggttggc
      661 ccgctgtcct cggtcccgcg cggccgggct tgccaggtct tggcggcgac gcagcgtcac
      721 gctgggccgg tcgggtcggc ggtgatgcca ggcggcctcg atcaacaggc gccgagcgtg
      781 agcgttgccg gttttggtga tcgagccctg tgaacgggac cctccggagg aggactcggt
      841 gggtaccagt ccgaggtagg ccccgatcgt gcggccggtc agccgttgcc agtccccgat
      901 ctcgaccgcg agcccgaacg ccgtcaacgt cgacaccccg cgcaggcacc ccagccgggt
      961 caccaccggg gcgtactcgc tgctggtcgc catctgggcg atcgcggcgt ccaaacggtc
     1021 gcggcgggcg agggtggcca gcacggtgtc gtaggcgata tcgaaggcca gacccagccc
     1081 cggctgatcg aacaccgcca gccgacgacg ggcctgcagc cagcgatcgt ggttaccggt
     1141 ccagggcttc ccgccgtagt agacgatccc ttggcgcagc agcagtttcg acaaccggtg
     1201 ccgggcagcg agcagatccc ggcggacgtc ttcacgggcc cggaccagat cgcgggcggc
     1261 ttcctgtccg acgctgggca ccgtcaccga gacgatctcg tccagatgca gcagtcgggc
     1321 cagatgccgg gcgtcgcggc ggtcggtctt gacccggtca cctgacgggc gctgcagccg
     1381 cgatggtgcg gccactcggc atgagatccc gtgcccgacc agggagcggg ccagcagacg
     1441 ttcaaggacg cgcagaacgg cagtcgaggt cgcaccgtcg cggcggctgg aggccccttg
     1501 gctccaccat cggcggcgcg gcggtgatcc gggctgctgg tcgacgacac tgctgccgtc
     1561 cgtgcgtgcc tgacttcgct gctcgaatcg gtcggcgagt tcagcgtggt gggccagtgc
     1621 gccgactgcg ccgagtgcgt cgaggcagcg accgtctggc gccgcacgtg atcgcaatgg
     1681 acgcggccgt gcccggcatc gacgggatcg ccgcgaccag tcggctcctg ccgtcgcgct
     1741 cccggttccc gggtcctgtt cctgacatcc tcggtggcgg gcgacacgat ccgtcgggcg
     1801 tacgccgcag gtgccgtcgg ctacctgtcg aagtcggaca cgagttcggg cttccgtacg
     1861 agcacgtctg cgatctgtca caagacttac cggaaatcgt tctcaactag gcagacgcgc
     1921 ggtgagtcgg taccgcgcca tcgtgtggca cacaatggcc agacgcgcgg gcgggccggc
     1981 ccgccggccg agctggttga acggatcatc caggaagcgg gacgggaacg accgtcagcc
     2041 catcgatgcc cgcgaagtca tccggattgc tggtgtgcac gggcaggccg cgggcgagcg
     2101 ccaccgcggc gatcatcgcg tcgtacgcgc gcgcctgggg cttgcgcccg ctgcgccgca
     2161 gcgcggcggc caccctgccg aacgcccgcg ctgccgcggc gtcgaagggc aacgggtcga
     2221 agtctgcctc ggcctgctgc aggaccgcct gtcggtcggc gcggtcccgt tccgaggtgg
     2281 cgaccagcgg gcctaccgac aactccgcga gcgtgatggt cgagatcagt ggcacgtcgg
     2341 gcagcgccgt cgcagcggtc agacgcggga ggttgatcac cgtgctggtg tccaggagcc
     2401 ccgccaccgg agcggtcaca gcgacgggtc cagcaccgcg tccacgtcga cgcggaaggc
     2461 ctgtgtatcg accgccggga ccgagcgcca ccgcgccagc agcgtgcttg ccgtgagccg
     2521 aggacgtggc agcggccgca gctccccgac cggcactccg gaacgagtca cggtcaatgt
     2581 ctcgccatgc tcgacccggt ccatcacttc cccgccgtgg ttgcggagat cgcggatcgt
     2641 gacctcgtcc atggcatgac tgtatcacgc gtgagacgca gctagccgtc gacgcttgta
     2701 ctgcggaaaa cgcggcacgt tccgcccgga ccgccggctc g
//
LOCUS       DASYLX010000889         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1590380,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000889 DASYLX010000000
VERSION     DASYLX010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590380"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VGP27_20770"
     CDS             complement(<1..1167)
                     /locus_tag="VGP27_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853765.1"
                     /translation="MTYDVILRNGLWFDGTGSRPQRRTLGIRDGIVAAVSAAPLDETG
                     CPDVIDAEGKWIAPGFIDVHTHYDAEVLLDPGLRESVRHGVTTLLLGNCSLSTVYADS
                     EDAADLFSRVEAVPRKFVLGALEAKRTWTSAAEYVQTLDDLPLGPNVSSMLGHSDLRT
                     SVLGLQRATSEVKPTGAELERMATLLDEALDAGLLGMSGMDAPIDKLDGDRFRSRALP
                     STFATWRERRRLIKVLRKRGRILQSAPNTKNPLEALNFFLTSSGMFGRRRGVRMSLLV
                     SADAKSNTGAVHVFGPGTRILNRLLNAFVRFQHLPVPFELYSDGIDLPVFEEFGAGTA
                     ALHLRDQMERNELLADPQYRRRFRASFDRKKLGPTLWHRDLHDATIVECPDKTLI"
     gene            1231..1617
                     /locus_tag="VGP27_20775"
     CDS             1231..1617
                     /locus_tag="VGP27_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853766.1"
                     /translation="MTPPVIARWIDIIENGRTSELESLLAEDAVFYSPAVFRPQEGRD
                     TTLAYLTAAAKVFGDTDFHYVEKWYGERSAILEFAATIDGKYVNGIDMIHWNDAEQIV
                     SVKVMVRPLKGLQALMPKMAELLATS"
     gene            1620..2126
                     /locus_tag="VGP27_20780"
     CDS             1620..2126
                     /locus_tag="VGP27_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7853767.1"
                     /translation="MRLLLIADTHVPKRARDLPDRVWDEVARADVVVHAGDWVEPALL
                     DALEARAARLVACWGNNDGEELRARLPERADVTLGGVRFTVVHETGGSGGRDSRMAKQ
                     YPNTDVLVFGHSHIPWDSTATTGLRLLNPGSPTDRRRQPFCTYMTANVDDGVLSDVTL
                     HRLERRGA"
     gene            2119..>2316
                     /locus_tag="VGP27_20785"
     CDS             2119..>2316
                     /locus_tag="VGP27_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853768.1"
                     /translation="MRDRPAKIVDVHEIASGMPHATRIEGPKGNPIYQVGGKSFVFFR
                     TPQPDAEDPDTGERYADVIMIW"
BASE COUNT          401 a          730 c          806 g          379 t
ORIGIN      
        1 gatcagcgtc ttgtccgggc actccacgat cgtggcgtcg tggaggtcgc ggtgccacag
       61 cgtcgggccg agttttttgc ggtcgaacga agcgcggaag cgtcggcggt actgcggatc
      121 ggcgagcagt tcgttgcgct ccatctgatc gcgaaggtga agggccgcgg tgcctgcgcc
      181 gaactcctcg aagaccggca ggtcgatgcc gtcggagtac agctcgaacg gcacaggcag
      241 atgctggaac cggacaaacg cgttgagcag tctgttgaga atccttgttc ccgggccgaa
      301 tacatgcacg gcaccggtgt tggacttggc atcggcggag acgagcaggc tcatccggac
      361 gccacgccga cggccgaaca tcccgctgct ggtgaggaag aagttcagcg cctcgagggg
      421 gttcttcgtg ttgggcgcgc tctggaggat gcggccgcgc ttacgcagca ccttgatcag
      481 cctccggcgc tcacgccagg tcgcgaaggt cgacggcagc gcgcgggacc ggaagcggtc
      541 accgtcgagt ttgtcgatcg gcgcatccat accggacatg ccgagaaggc cggcgtcgag
      601 cgcctcgtcg agcaacgtgg ccatccgctc gagttcggca ccggtcggtt tgacctctga
      661 ggtggcacgc tggagtccga ggaccgaggt gcgcaggtcc gagtggccga gcatggaact
      721 gacgttcggc ccgagcggca ggtcgtcgag ggtctgcacg tattcggctg ccgacgtcca
      781 tgtcctcttg gcttccaggg cgccgagcac gaacttgcgg ggcaccgctt cgacgcggct
      841 gaacagatcc gcggcgtcct cggagtcggc gtacaccgtc gacagcgagc agttgcccag
      901 caggagcgtc gtcacgccat gccgcacgga ctcccgcaag ccgggatcga gcagcacctc
      961 ggcgtcgtag tgcgtatgca cgtcgatgaa gccgggggcg atccatttgc cttcggcgtc
     1021 gatgacgtcc gggcagcccg tctcgtcgag tggtgccgcg gacaccgcgg caacgatgcc
     1081 gtcgcgaatg cccagcgtgc ggcgctgcgg gcggctgccg gtgccgtcga accacaggcc
     1141 gttgcgaagg atcacgtcgt aggtcatggc gacgaccgta ccaaagatag tgagtgctta
     1201 caatcttttt ctgacgctag ggtcgtaagc atgacgccac cggtcatcgc gcgctggatc
     1261 gacatcatcg agaacggccg cacttcggag ctggaaagcc tgctcgccga ggacgcagtg
     1321 ttctactcgc ccgccgtgtt caggccgcag gaaggccgcg acacgacgct ggcatatctg
     1381 accgcggctg caaaggtctt cggcgacacc gacttccact acgtcgagaa gtggtacggc
     1441 gagcggtcgg cgattctcga gttcgccgcg accatcgacg ggaagtacgt caacggcatc
     1501 gacatgatcc attggaacga cgccgagcag atcgtgtcgg tcaaggtgat ggtgcgcccg
     1561 ctcaagggac tgcaggcgct catgcccaag atggccgagc tgctcgcgac gagctgaccg
     1621 tgcggctgct gctgatcgcc gacacccacg tgccgaagcg ggcccgggac ttgccggacc
     1681 gggtgtggga cgaggtggcc agagcagacg tcgtcgtgca cgccggcgac tgggtagagc
     1741 ccgcactgct cgacgcgctg gaggccaggg ccgctcgcct cgtcgcgtgc tggggcaaca
     1801 acgacggtga ggagttgcgg gcgcgtctgc ccgaacgcgc cgacgtcacg ctcggcggtg
     1861 tgcggttcac ggtggttcac gagaccggtg gttccggcgg gcgggattcg cgaatggcga
     1921 agcagtatcc caacaccgac gtcctggtgt tcgggcacag ccacatcccg tgggacagca
     1981 cggccacgac cggactgcgc ctgctcaacc cgggatcgcc gaccgaccgc cgccgccagc
     2041 cgttctgcac ctatatgacc gcgaacgtcg acgacggtgt gttgtccgac gtcaccctgc
     2101 atcgcctgga gcgccgtggt gcgtgaccga ccggcgaaga tcgtcgatgt gcacgagatc
     2161 gcctccggca tgccgcacgc cacgcggatc gagggcccga agggcaatcc catctaccag
     2221 gtgggtggga agtcgttcgt gttcttccgc acgccgcagc ccgacgccga ggatcccgac
     2281 acgggggagc gctacgccga cgtgatcatg atctgg
//
LOCUS       DASYLX010000890         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1808881,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000890 DASYLX010000000
VERSION     DASYLX010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808881"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGP27_20790"
     CDS             <1..285
                     /locus_tag="VGP27_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEV7853769.1"
                     /translation="LHQYLIGLRRRHPWLHTAETSAVVLTNTQYVYRTRRGSDALLVA
                     LNIDDAPMSLSLSEFGYADGRIVAGSGAPPQDVITRAEIEPHGWLIIAPS"
     gene            complement(289..723)
                     /locus_tag="VGP27_20795"
     CDS             complement(289..723)
                     /locus_tag="VGP27_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEV7853770.1"
                     /translation="MDNMTDDPYELRRFVDAQVGIYDTVIDELTAGRKRSHWMWFVFP
                     QLRGLGSSPTAVRFGITSTDEARAYLAHDLLGPRLRECATLVAGIDGRSAEEIFGRPD
                     DMKLRSSMTLFARATEDNADFVAVLDKFYGGEEDPATLARLS"
     gene            958..3018
                     /locus_tag="VGP27_20800"
     CDS             958..3018
                     /locus_tag="VGP27_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7853771.1"
                     /translation="MLLFTSILSAAVVGFIGYQSGRSSLRAAVFDGLTEIRGSQARQL
                     EGQFRDLENSLVVWSRGSTTADAIDAFTAGFDQLGSATINPAQQQSIADYYTDNFAKS
                     EERQTGNKVDVATLLPASNAERYLQAYYTAPFNDWDVAINNDDAHDGSAWSAANVRFN
                     EFFREMVNRFEFEDVLMLDTRGNVVYSAYKGVDLGTNILTGPYREGELRDAYQKALAA
                     NVVDYVGITDFGDYLPADEPTAWLVSPVGPPGRVDGVLALQFPIFKINGLMTADQKWE
                     QAGLGKTGETFLVGPDDLMRSDSRLFLEDPDAYKREAIEAGTPPDVAEKSIRQHGTTL
                     VQPVATEATRLAQRGQSGTLVADDYLGRETLQTYAPVNLPGLHWSVVAKIDTAEAFEP
                     VTRFTRTLVLSTVVIIFVVCVAAMLLARLFVRPIRRLEAGAQRISTGDYEVTLPVQSR
                     DEFGDLTVAFNDMSRNLAIKEELLAEQRRENDRLLLSLMPEPVVQRYREGEETIAQDH
                     QNVTVIFADIVGLDELSSELTSDEGLSIVNKLVRQFDSAADNLGVERVRTLHNGYLAS
                     CGMSVPRLDNVRRTVDFAVEMQHIIDRFNSESGNDLKLRAGIDTGTVSSGLVGRASLA
                     YDMWGGAVNVAYQVQSGSARPGIYVTSRVYDVMRDSRHFTPAGEVSGEDGPEPIWQVT
                     EHKS"
     gene            3015..>4119
                     /locus_tag="VGP27_20805"
     CDS             3015..>4119
                     /locus_tag="VGP27_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV7853772.1"
                     /translation="MSVLDSSWFYWALVVAIGLPVGLVILTELQNALARRGSNLTRPV
                     HLVRNYLLPLGALLVLLTKATQVEAGATTVRIVATVFAFVVLVLLLSSMNATLFQGAP
                     EGTWRHRIPSIFLDVARFAVIAVGLALILAYIWGANVGGLFTALGISSIVLGLALQNS
                     VGQIISGLLVLFEQPFQLGDWVDTPSARGRVVEVNWRATHIDTGTGLQIMPNSVLAAA
                     SFSNLSRSPGGHTIAVTTVFSIADAPDEVCAVLTRVATALAQLRPDAVPSTVAAGGNN
                     YTTSIPLRSPADDMATRSVFLRWVWYASRRAGLHLDEAEDDFSTPDRMADALRAIAPT
                     LRLTHTDQQVLAAHARITRYGADEYIQFAGQVPE"
BASE COUNT          724 a         1340 c         1292 g          763 t
ORIGIN      
        1 ctgcaccagt acctgatcgg attgcgccga cgtcatccgt ggttgcacac cgccgagacg
       61 tcggccgtcg tgctgaccaa cacgcagtac gtgtaccgga cgcgccgcgg ctcggacgcg
      121 ctgctcgtcg cactcaacat cgacgatgct ccgatgtcct tgtcgctgag cgagttcggg
      181 tatgccgacg ggcggatcgt cgcgggttcc ggtgcaccgc cgcaggacgt gatcacccgg
      241 gccgagatcg agccgcacgg ttggctgatc atcgcgccga gctgaccctc aggacagccg
      301 cgccagcgtc gctgggtcct cctcgccgcc gtagaacttg tccagcaccg cgacgaagtc
      361 ggcgttgtcc tcggtcgctc tggcgaacag cgtcatcgac gagcgcagct tcatgtcgtc
      421 gggtcggccg aagatctcct cggccgagcg gccgtcgatc ccggctacca acgttgcgca
      481 ctcccgcagc cgtgggccga gcaggtcgtg ggcgaggtag gcgcgggcct cgtcggtcga
      541 cgtgatcccg aagcggaccg ccgttgggct gctgccgagg ccgcgcagtt gcgggaacac
      601 gaaccacatc caatggcttc tcttgcggcc tgcggtcaat tcgtcgatga cggtgtcgta
      661 aataccaact tgtgcgtcca caaaccggcg caattcatac ggatcgtcgg tcatgttgtc
      721 catcccaata tctcgcgatt tcctcactgt gatgatggct tcaagtttca ccaagtagtc
      781 ttcgcccggc agccgatttc ttgggaaagg acgcccatgc cgcctttgtc cacgggccgc
      841 aacggtgcgc aggcgacgac cgaggaacac accggggtgg agccggcttc cgggacgggc
      901 cgattccgca ggcgaaattt gctgtcgaga gtcagtatcc aatccaagct gttggtcatg
      961 ctgctgttca ccagcatctt gtcggcggcc gtcgtcggct tcatcggcta ccaatccggc
     1021 cgcagctcgc tgcgcgcggc ggtgttcgac ggactgaccg aaatccgggg ttcgcaggcg
     1081 aggcaattgg aggggcaatt cagagacctc gagaattcac tggtcgtgtg gtccaggggg
     1141 tccaccactg ccgacgcgat cgacgcgttc accgccggtt tcgatcagct cggcagcgcc
     1201 accatcaatc ccgcccagca gcagtccatc gccgactact acaccgataa tttcgccaag
     1261 tcagaggaac ggcagaccgg caacaaagtc gacgtcgcca cgctgctgcc ggcctccaac
     1321 gccgagaggt acctgcaggc ttactacacc gcgccgttca acgactggga cgtcgcgata
     1381 aacaacgacg acgcgcacga cggcagcgcc tggtcggcgg ccaacgtccg cttcaacgag
     1441 ttcttccggg agatggtgaa ccggttcgag ttcgaggacg ttctcatgct cgacaccagg
     1501 ggcaacgtgg tgtacagcgc ctacaagggt gtcgacctcg gcaccaacat cctcaccggt
     1561 ccgtatcgtg agggcgaact acgcgacgct taccagaagg cgctggccgc caacgtcgtc
     1621 gactacgtcg gcatcacgga cttcggtgat tacctgccgg ccgacgaacc gaccgcctgg
     1681 ctggtgtcgc cggtgggtcc gccaggacgc gtggacggcg ttctggcgct gcaatttccg
     1741 atattcaaga tcaacgggct gatgacggca gaccagaagt gggaacaagc cggcctgggt
     1801 aagacgggtg aaaccttcct ggtcggtccc gatgacctga tgaggtcgga ttcgcgattg
     1861 ttcctcgaag atcctgacgc ctacaagcgg gaggccatcg aggcgggtac acctccggac
     1921 gtggcggaga aatcgatccg ccagcacggc accacgttgg tgcaaccggt ggccaccgag
     1981 gccacccggc ttgcgcagcg cggccaaagc ggaaccctgg ttgccgatga ctacctcggc
     2041 cgcgagacgc tccagacgta tgcgccggtg aacctgcccg gtttgcattg gtcggtggtc
     2101 gcgaagatcg acaccgccga ggcattcgag ccggtgacga gattcacccg gaccttggtg
     2161 ctgtccacgg tggtgatcat cttcgtggtg tgtgtggcgg cgatgttgct ggcccgattg
     2221 ttcgttcggc cgatacggcg cctggaggcg ggggcgcaac gaatcagcac gggagattac
     2281 gaggtaactc tgcctgttca gtcccgcgac gaattcggcg atctcacagt cgctttcaac
     2341 gacatgagtc gtaacttagc catcaaggag gaattgctcg cggagcagcg cagagagaac
     2401 gaccggctgc tgctctcctt gatgcccgag ccggtggtgc agcggtaccg cgagggtgag
     2461 gagacgatcg ctcaggacca ccagaacgtc accgtgatct tcgccgacat cgtcggcctg
     2521 gacgagctgt cctcggagct gacttctgac gaagggttgt ccatcgtcaa caagctcgtt
     2581 cgccagttcg actccgccgc agacaatctc ggtgtggaga gggtacggac gctgcataac
     2641 ggctacctcg ccagctgcgg catgagcgtc ccgcggctgg acaacgtgcg ccgtacggtc
     2701 gacttcgccg tcgaaatgca acacatcatc gatcgcttca acagcgagtc cggtaacgat
     2761 ctcaagttgc gggccggaat cgataccggc accgtgtcga gcggactcgt cggtcgggcc
     2821 agcctcgcct acgacatgtg gggcggagcg gtgaatgtgg cctaccaggt gcagagcggc
     2881 tctgcacgac cgggcatcta cgtcacctcg cgggtgtacg acgtcatgcg tgactcccgc
     2941 cacttcaccc cggccggcga agtcagcggt gaggacggtc ctgagcccat ctggcaggtg
     3001 acggagcaca agtcatgagc gtgctggatt cctcgtggtt ctactgggcg ctcgtcgtgg
     3061 cgataggcct gcccgtcggc ctggtgatcc tcaccgaact gcagaacgca ttggcgcgcc
     3121 gtggcagcaa cctgaccaga ccggtacacc ttgttcgcaa ttaccttctg ccccttggtg
     3181 ccctgctcgt cctgctgaca aaggccactc aggtggaggc gggcgcgaca acggtgcgca
     3241 tcgtcgccac ggtgttcgcc ttcgtcgtcc tggtcctgct gctctccagt atgaacgcga
     3301 ccctgttcca gggcgctccg gaaggtacct ggcgccaccg cataccgtcg atcttcctcg
     3361 atgtcgcacg cttcgcggtc atcgcggtgg ggctggccct catcctggcc tatatctggg
     3421 gggccaacgt cggcggcctg ttcaccgccc tcggcatcag ctccatcgtt ctgggccttg
     3481 cgctgcagaa ctccgtcgga cagatcatct ccggactcct cgtcctgttc gagcagccat
     3541 tccagctcgg cgactgggtc gacaccccgt cggcgcgggg gcgtgtcgtc gaagtgaact
     3601 ggcgcgcaac tcatatcgac accggtaccg gtctgcagat catgcccaac tcggttttgg
     3661 cggcggcgtc gttcagcaac ctgagcaggt cacccggtgg ccacacgatc gcggtcacca
     3721 ccgtcttctc gattgccgac gcacccgacg aggtctgtgc ggtgctgacc cgcgtcgcca
     3781 ctgccttggc gcagctgcgg ccggatgcgg tgccctccac tgtggccgca ggaggcaaca
     3841 actacacgac gtcgatcccg ctccgttccc ccgccgacga catggcgacg cgttcggttt
     3901 tcctgcgctg ggtctggtat gcgtcacggc gcgccggctt gcacctcgac gaagcggagg
     3961 acgacttctc gacgccggac cggatggccg atgcgctgcg tgccatcgct ccgactctac
     4021 gattgaccca caccgaccag caggtgctcg ccgctcatgc gcgcatcacc cgctacggcg
     4081 ccgacgagta catccaattc gccggtcagg ttccggagc
//
LOCUS       DASYLX010000891         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_491093,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000891 DASYLX010000000
VERSION     DASYLX010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491093"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VGP27_20810"
     CDS             <1..886
                     /locus_tag="VGP27_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7853773.1"
                     /translation="NMLRTTVAAFGAGIGGADTVLVQPFDAAIPGGLPGTAASFARRI
                     ARNTQLLLLEESHLGRVLDPAGGSWFVEDLTEQLAQKAWEHFQQIEAEGGFAAARDFV
                     AAQIADVRDRRTDDINHRRKALTGVNEYPNLNEAPLPHGDSVSAVARYAAGFEALRDR
                     SDGWLDKTGSRPKVLLLPLGPLAEHNIRVTFAANLLASGGIEAVNPGPVNAAGVADAV
                     SAAGGPGVVVICGTDARYRTEAAGVVEAARKAGVSLVLLAGPDKAVADADHKPDDYLT
                     AKVDAVDALSDLLTRLGA"
     gene            889..3153
                     /gene="scpA"
                     /locus_tag="VGP27_20815"
     CDS             889..3153
                     /gene="scpA"
                     /locus_tag="VGP27_20815"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEV7853774.1"
                     /translation="MTVNDVAGKAAPEATGIGSFADVPLRSERAAEPATHAAVTAHVA
                     AAAEAHGYTPEQLDWSTPEAIDVKPFYIAADRDDAVKAGYPLDTFPGAPPYLRGPYPT
                     MYVNQPWTIRQYAGFSTAAESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGD
                     VGMAGVAIDSILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVPPEKLA
                     GTIQNDILKEFMVRNTYIYPPKPSMRIISDIFGYTSVRMPKFNSISISGYHIQEAGAT
                     ADLELAYTLADGVEYIKAGLDAGLSIDKFAPRLSFFWGIGMNFFMEVAKLRAGRLLWS
                     ELVAQFEPEDAKSLSLRTHSQTSGWSLTAQDPFNNVARTCIEAMAATQGHTQSLHTNA
                     LDEALALPTDFSARIARNTQLLLQQESGTIRPIDPWGGSYYVEWLTHQLTERVRAHIR
                     EVNEHGGMAQAIDDGIPKMRIEEAAARTQARIDSGQQPVIGVNKYKVAAGDGTDHEIE
                     VLKVENSRVRAEQLAKLEKLRAERDQAVVDAALADLTKAADGEGNLLELAINAARANA
                     TVGEISDALERVWGRHQAEIRTIAGVYRDEVGKGSNIASATELVEKFAEADGRRPRIL
                     VAKMGQDGHDRGQKVIATAFADIGFDVDVGSLFSTPEEVARQAADNDVHVVGVSSLAA
                     GHLTLVPALRDALAEVGRPDIMVVVGGVIPPGDFEDLYAAGATAIFPPGTVIADAAIG
                     LLHKLAERSGYDLG"
     gene            3154..>3723
                     /gene="meaB"
                     /locus_tag="VGP27_20820"
     CDS             3154..>3723
                     /gene="meaB"
                     /locus_tag="VGP27_20820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV7853775.1"
                     /translation="MADRIDDLAAAIRNRDRAALPRAITLVESTRADHREQAQQLLME
                     LMPEAGAAMHVGITGVPGVGKSTTIEALGMYLIGQGHRVAVLAVDPSSTRTGGSILGD
                     KTRMPRLAVQPDAYIRPSPTSGTLGGVARATRETIVLLEAAGFDVILVETVGVGQSEV
                     AVANMVDTFVFLTLARTGDQLQGIKKGVLE"
BASE COUNT          641 a         1260 c         1251 g          571 t
ORIGIN      
        1 gaacatgctg cgcaccaccg tcgcggcctt cggcgccggt atcggcggcg cggacaccgt
       61 gctggtgcag ccgttcgacg cggcgatccc cggcggactg cccggcaccg cagcgagttt
      121 cgcccgccgc atcgcccgca atacgcaact gctgctgctg gaggagtcac acctggggcg
      181 ggtgctcgac ccggcgggcg gctcgtggtt cgtcgaggac ttgaccgagc agctcgcgca
      241 aaaggcgtgg gagcacttcc agcagatcga ggcggagggc ggtttcgcag ccgcccgtga
      301 cttcgtcgcc gctcagatcg ccgatgttcg cgaccggcgc accgacgaca tcaaccaccg
      361 ccgcaaggcc ctcaccggtg tcaacgagta cccgaacctc aacgaagcgc cgctgccaca
      421 tggtgattcg gtgagcgccg tcgcgcgata cgccgcaggc ttcgaggcgt tgcgcgatcg
      481 ctccgatgga tggctggaca agacggggtc gcggccgaag gtgctgttgc tgccgctggg
      541 cccgctggcg gagcacaaca tccgggtgac gttcgccgcc aacctgctcg cctcgggtgg
      601 catcgaggca gtcaatcccg gtcccgtcaa cgccgcgggg gtggcggacg ccgtgtcggc
      661 agcgggcggc ccaggcgtcg tggtgatctg cggcaccgac gcccggtacc gcaccgaagc
      721 cgcgggggtg gtcgaggccg cacgaaaggc cggggtgtcg ctcgtcctgc tggccggacc
      781 cgacaaggcg gtcgccgacg ccgaccacaa gcccgacgac tacctgaccg ccaaggtcga
      841 cgcggtcgac gccctgtcgg atctgctcac tcgattgggg gcatgacgat gactgtcaat
      901 gacgtcgccg gcaaagccgc tccggaagca accggaatcg gcagcttcgc cgacgttcct
      961 ctacgcagcg agcgggccgc cgagcccgca acgcacgccg ctgtcaccgc acacgtggcc
     1021 gccgcggccg aggcgcacgg ctatacgccg gagcagctcg actggtccac ccccgaggcc
     1081 atcgacgtca agccgttcta catcgccgcc gaccgggacg acgccgtgaa ggcgggctac
     1141 ccgctcgaca ccttccccgg tgcgccgccg tacctgcgcg gcccctatcc gacgatgtac
     1201 gtcaaccagc cgtggaccat ccggcagtac gccggtttct ccaccgccgc ggagtccaac
     1261 gcgttctacc gacgcaacct ggccgccggg cagaagggtc tttcggtggc gttcgacctg
     1321 gccacccacc gcggctacga ctccgaccac ccccgcgtcg ccggtgacgt cggaatggcg
     1381 ggcgtggcaa tcgattccat cctcgacatg cggcagctgt tcgacggaat cgacctttcg
     1441 accgtgtcgg tttcgatgac gatgaacggt gcggtgctgc cgatcctcgc gctgtacgtg
     1501 gtggccgccg aggagcaggg cgtgccacct gagaagttgg ccgggaccat ccagaacgac
     1561 atcctcaaag agttcatggt ccgcaacacc tacatctacc cgccgaagcc gtcgatgcgg
     1621 atcatctccg acatcttcgg ctacaccagc gtgcggatgc cgaagttcaa ctcgatctcc
     1681 atctccggct accacatcca ggaggctggt gcgacagcag atttggagct ggcgtacacg
     1741 ctggccgacg gagtcgagta catcaaggca ggtttggacg cgggcctgtc catcgacaag
     1801 ttcgcgccgc ggctgtcctt cttctggggt atcgggatga acttcttcat ggaggtcgcc
     1861 aaactgcgcg ccggccgctt gctgtggagt gagctggtcg cccagttcga accagaggat
     1921 gccaaatcgc tttcgctgcg cacgcattcg cagacgtcgg gttggtcgtt gacggcgcag
     1981 gatccgttca acaacgtcgc ccgcacctgc atcgaggcga tggcggcgac gcagggacac
     2041 acgcagtcgc tgcacaccaa cgcgctcgac gaggccctgg cgctgcccac cgatttctcc
     2101 gccaggatcg cgcgcaacac gcagctgctg ctgcaacagg agtccggcac catccggccg
     2161 atcgacccat ggggcggttc gtactacgtg gagtggctca cccatcagct caccgagcgg
     2221 gtgcgcgccc atatccgtga ggtgaacgag cacggcggca tggcacaggc catcgacgac
     2281 ggcatcccca agatgcgcat cgaggaggct gccgcacgca cccaggcccg catcgattcc
     2341 ggccagcagc cggtgatcgg cgtcaacaag tacaaggttg ccgccggcga tggcaccgat
     2401 cacgaaatcg aggtgctcaa ggtcgagaac agccgggtcc gcgctgagca gctcgccaag
     2461 ctcgagaagc tgcgtgccga gcgcgaccag gccgtcgtcg atgcggcgct ggccgacctg
     2521 acgaaggcgg ccgacggtga gggaaacctg ttggagctcg ccatcaacgc ggcacgcgcg
     2581 aacgccaccg tcggcgagat ctccgatgcg ttggagcgag tctggggacg gcaccaggcc
     2641 gagatccgta ccatcgccgg ggtctaccgc gacgaagtcg gaaagggcag caacatcgcc
     2701 agcgccaccg agctagtcga gaagttcgcc gaggccgacg gccgacggcc gcgaattttg
     2761 gtggccaaga tgggtcagga cggtcacgat cgggggcaga aggtcatcgc gaccgcgttc
     2821 gccgacatcg gtttcgacgt cgacgtcggc tcactgttct cgacgccaga agaggtggcg
     2881 cgtcaggccg ccgacaacga cgtgcacgtg gtgggggtgt cgtcccttgc cgccggacat
     2941 ctgacgctgg tgcccgcgct gcgcgacgcg ctggccgaag tcgggcgacc cgacatcatg
     3001 gtcgtggtgg gcggggtgat cccgcccggt gatttcgagg acctgtacgc ggcgggtgcc
     3061 accgcgatct tcccgcccgg caccgtgatt gccgacgccg cgatcggctt gttgcacaag
     3121 ctggccgagc gctcgggata cgacctcggc tagatggccg accggatcga cgacctcgct
     3181 gccgcgatca ggaatcgtga tcgcgcggcc ctgccgcggg ccatcaccct ggtggagtcc
     3241 accagggccg atcaccgcga gcaggcccaa cagctgttga tggagttgat gcccgaagca
     3301 ggtgctgcga tgcacgtcgg catcaccggc gttcccggcg tgggcaagtc gaccacgatc
     3361 gaggcgctcg ggatgtacct catcggacag ggacatcggg tggcggtgct ggccgtcgac
     3421 ccgtcgtcga cgcgcaccgg cgggtcgata ctcggcgaca aaacccggat gccgcgcctg
     3481 gccgtgcaac ccgacgccta tatccggccc tcgcccacgt ccggcacgct gggtggcgtc
     3541 gccagagcca cccgggaaac catcgtgttg ctcgaggcgg caggcttcga cgtgattctg
     3601 gtggaaaccg tcggcgtcgg gcagtccgag gtggcggtcg ccaacatggt ggacacgttc
     3661 gtgttcctga ccctcgcccg caccggtgat cagctccagg gcatcaagaa gggtgtgctg
     3721 gag
//
LOCUS       DASYLX010000892         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2358074,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000892 DASYLX010000000
VERSION     DASYLX010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358074"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGP27_20825"
     CDS             <1..402
                     /locus_tag="VGP27_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853776.1"
                     /translation="VGLGLLLTRCPANRDGMPGQLAQAMEETTAAARSGALALDMWAQ
                     RRSSAQLTSVQLTDARDEVIKAYKTIAQLRAEDPVDIERQRELTVSMTTIIAELNAAS
                     GTVRDVTTEPSSDKARAELATSADALESEYR"
     gene            402..1631
                     /locus_tag="VGP27_20830"
     CDS             402..1631
                     /locus_tag="VGP27_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV7853777.1"
                     /translation="MGKLFSVALGILTAIGGFLDIGDLVTNAIVGSRFGLALAWVVPV
                     GVLGICLYANMAGRVAAVSGRATFEIIRERLGPRAAAANLSASFFINMMTLTAEIGGV
                     ALALQLATDVGRLMWIPVAAFAVWLVIWRVKFSIMENVTGLLGLCLIVFAVSVFALHP
                     NWGDLVHQATAPVIPQQESSATYWYYAVALFGAAMTPYEVFFFSSGAVEEHWTAKDLG
                     TSRLNVMIGFPLGGILSIAIAACATIVLLPNQIEVTSLSQTVMPVVAAGGKLALAFVI
                     IGIVAATFGAALETTLSGGYIVAQFLGWPWGKFRRPAQAARFHVVMFVTIVVGAAVLF
                     TGVDPIMVTEYSVVFSAIALPLTYLPILIVANDAQYMGDDTNGKVTNMFASVYLAIIL
                     AASLAAIPLMIVTGAGQ"
     gene            1628..2032
                     /locus_tag="VGP27_20835"
     CDS             1628..2032
                     /locus_tag="VGP27_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853778.1"
                     /translation="MSADELPVKGHRGSRLVDARLHLLDRQLLDEHDDPVGIVDDLEL
                     SGAEFGEEIAAGSEAPRVTALLSGRVVVTRIFGGAPPRSLLQEIPWRLVESVGVVVKL
                     KATDMTFDVNWVEQWLRDHVIRHLPGGRHAAE"
     gene            2019..2360
                     /locus_tag="VGP27_20840"
     CDS             2019..2360
                     /locus_tag="VGP27_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853779.1"
                     /translation="MQLSRFLGLRVVDAGNHPVGTVTDVRLAIGGDLQHDPSTPRVLG
                     LVISPRTRSSFLGYERSTATAPFLLAQLLRWRHRGTFLAGWEDVAKVGDDLVVLRPRY
                     TRYSAVLPAGD"
     gene            2476..2712
                     /locus_tag="VGP27_20845"
     CDS             2476..2712
                     /locus_tag="VGP27_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV7853780.1"
                     /translation="MTEKNSGPQEAVKGAVEDVKGKAKEAVGTVTGRNDLVDEGKAQQ
                     DKADAQQNAAKKEAEADAARGSAKAAEKRQESNQ"
     gene            complement(2780..3088)
                     /gene="usfY"
                     /locus_tag="VGP27_20850"
     CDS             complement(2780..3088)
                     /gene="usfY"
                     /locus_tag="VGP27_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein UsfY"
                     /protein_id="HEV7853781.1"
                     /translation="MKSPKDPVDHARTTRPHAGESMTDTKNMPALIVIGVALACFVGG
                     LAAHASNHHSTGLVFGSLSAAFLIVGLLWLYVEHRRVRGIEQRWYAEHPEAHRQRPSS
                     "
     gene            complement(3176..3802)
                     /locus_tag="VGP27_20855"
     CDS             complement(3176..3802)
                     /locus_tag="VGP27_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7853782.1"
                     /translation="MPDNSERPLTAVALVCSLKRSPAESSSELMAEHVCQQLRSHGVK
                     TDSLRCADYSIAPGVEADMGDGDEWPKIRQRVLDADILLLSTPVWLGHPSSITQRVLE
                     RLDAELSNTDDEGRPIMYGKVAIVSVVGNEDGAHKVFADCAQGLNDVGFSFAAQANTY
                     WNGEAMQTTNYNDLDEVPEPVAKTTASAARNAAHLAGLLRAHQYPPQQ"
     gene            complement(3818..4165)
                     /locus_tag="VGP27_20860"
     CDS             complement(3818..4165)
                     /locus_tag="VGP27_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853783.1"
                     /translation="MRHDEARGRDGVFDAVRFSVGVAVAAVVFLVVATVWVSTCGGAT
                     FDTAACGAPQRTLLALGAPLILLYGGVRAFVRTYRIWRQNGITWPWQGAGWFLMAAML
                     LALVGSVPSIAMP"
     gene            complement(4323..4721)
                     /locus_tag="VGP27_20865"
     CDS             complement(4323..4721)
                     /locus_tag="VGP27_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853784.1"
                     /translation="MLTTQRSPPERPSAFRCHHLIKTFYAGFAWTERQHADGTITWTP
                     RPGIATAPIPTAAPCFPRWPNPPPTLGDITAPDPSPHRAMMMPTRRQTREQGRRDRIT
                     NKRRERTDRNAQEQRERQAWLAANDQPPPF"
     gene            5162..5734
                     /locus_tag="VGP27_20870"
     CDS             5162..5734
                     /locus_tag="VGP27_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV7853785.1"
                     /translation="MIPTRAKVYAAVGVISGVAAHLSGISAPVSVAVGLVAPVLLAAA
                     PRFLLATVRGAFTASPREASPTSAMSGMEFEDHVARIARSCGVPVIMTAVTGDWGVDL
                     VLGQRPERLAVQCKRQSRPVGAGAVQEVVAGAPMHGCTKTMVVTNHEFTRAAIKLAEL
                     HGCELVGGSELPRLRSILRRLTEPSAPTAP"
     gene            complement(5731..7032)
                     /gene="lat"
                     /locus_tag="VGP27_20875"
     CDS             complement(5731..7032)
                     /gene="lat"
                     /locus_tag="VGP27_20875"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HEV7853786.1"
                     /translation="MREVLSRSILADGLDFVLDIERSTGSILVDARTGDRYLDMFTFF
                     ASSALGMNHPALADDEAFRAELASAAVNKPSNSDVYSVPMARFVDTFARVLGDPALPH
                     LFFVDGGALAVENALKVAFDWKSRLNDARGTASSSSSSRAGSELGTKVLHLRGAFHGR
                     SGYTLSLTNTDPNKVARFPKFDWPRIDAPYIRPDADMDAVEAESLRQARAAFEAHPHD
                     IACFIAEPIQGEGGDRHFRPQFFAAMRELCDEFDALLIFDEVQTGCGLTGTAWAYQQL
                     GVAPDVVAFGKKTQVCGVMAGRRVDEVADNVFAVSSRINSTWGGNLVDMVRSRRILEV
                     IEADGLMEHAAEAGRYLHARLEELAAEFPESVRDVRGRGLMRAFSLPSREQRDDLIRK
                     LWDRQVVMLASGADSVRFRPALIVSYAEIDEAVAAVREALG"
BASE COUNT         1183 a         2235 c         2364 g         1263 t
ORIGIN      
        1 gtcggcctgg gcctgttgct cacgcgctgc ccggccaacc gggacggaat gcccggccag
       61 ctggcgcaag ccatggagga gaccaccgct gctgcgcgaa gcggcgcctt ggcattggac
      121 atgtgggcgc aacgacgctc gagcgcgcaa ctcaccagtg tgcagctgac ggacgcacgt
      181 gacgaagtca tcaaggcgta caagacaatt gcccaactgc gcgccgaaga ccccgtcgac
      241 atcgagcgac aacgtgaact gaccgtgtcg atgacaacga tcatcgcaga gctcaacgcg
      301 gcgtcgggga cggtccgcga tgtcaccacc gaaccgtcgt cggacaaggc gcgagccgag
      361 ctggcaacgt cggcggatgc gttggagagt gaataccgct gatgggcaaa ctcttctcgg
      421 ttgcgcttgg catcctcacc gcgatcggcg gcttcctcga catcggcgat ctggtcacca
      481 acgcgatcgt cggttcacgc ttcggtttgg ctttggcgtg ggtggtgccg gtcggcgtgc
      541 tgggcatctg cctctacgcc aacatggcgg gtcgggtggc ggccgtcagc ggccgggcga
      601 cgttcgagat cattcgggaa cggctcggcc cgcgtgcggc ggcggccaac ctgtcggcgt
      661 cgttcttcat caacatgatg acgctgaccg ccgagatcgg tggtgtggca ttggctttgc
      721 agctggccac cgacgtcggc cggctgatgt ggatacccgt cgcggcgttc gccgtctggc
      781 tggtgatctg gcgggtcaag ttctcgatca tggagaacgt gacaggtctg ctcggcctgt
      841 gcctgatcgt cttcgcggtc agtgtgtttg cgctgcatcc gaattggggc gacctggtgc
      901 accaggccac cgctccggtg attccgcagc aggagtcgtc ggcgacctac tggtactacg
      961 cggtcgcgct gttcggcgcc gcgatgacac cgtacgaggt gttcttcttc tcctccggcg
     1021 cggtcgagga gcactggacc gccaaggacc tcggcacctc gcggctcaac gtgatgatcg
     1081 gcttcccgct gggcggcatc ctgtcgatcg caatcgccgc gtgcgcgacg atcgtgttgc
     1141 tgcccaacca gatcgaggtc acatcgctgt cgcagacggt gatgccggtc gtcgccgcag
     1201 gcggaaagct ggcgctggcc ttcgtcatca tcggcatcgt ggcggcgacg ttcggtgcgg
     1261 cgctggagac cacgttgtcc ggcggctaca tcgtggcgca gtttctcggc tggccgtggg
     1321 gcaagttccg tcgccccgcg caggccgccc ggttccatgt ggtgatgttc gtgaccatcg
     1381 tcgtcggagc cgcggtgctg ttcaccgggg tggacccgat catggtcacc gaatactcgg
     1441 tcgtcttctc ggcgatcgcg ctgccgctga catatctgcc gatcctcatc gtcgccaacg
     1501 acgctcagta catgggcgac gacaccaacg ggaaggtgac caatatgttc gcgtcggtgt
     1561 atctggccat cattctggcc gcttcgctgg ccgcgatccc gctcatgatc gtgaccggag
     1621 cggggcaatg agcgccgacg agcttccggt caagggccat cgcggaagcc ggctcgtcga
     1681 cgcacgcctg cacctgctcg accgtcaact gctcgacgaa cacgacgacc cggtcggcat
     1741 cgtcgatgac ctcgaactgt ccggcgccga gttcggcgaa gagatcgcgg cgggctccga
     1801 ggcaccacgg gtgaccgcgc tgttgtcggg ccgagtggtg gtcacccgca tcttcggcgg
     1861 cgccccaccg cgctcgctcc tgcaggagat cccgtggagg ctggtcgagt cggtcggggt
     1921 cgtcgtcaag ctcaaagcga ccgacatgac attcgacgtc aactgggtcg agcaatggct
     1981 gcgcgaccac gtcatccgcc acctgcccgg aggccggcat gcagctgagt aggttccttg
     2041 gactgcgtgt ggtcgacgcc ggcaaccacc cggtcggcac tgtgaccgat gtgcggctcg
     2101 cgatcggcgg tgaccttcaa cacgacccct ccaccccgcg ggtgctgggt ctggtgatca
     2161 gcccacggac gagatcgtcg tttctcggct acgagcgcag caccgcaacc gccccgtttc
     2221 tgctggcaca gctgctgcgc tggcgacacc gcggcacgtt tctggccggc tgggaggacg
     2281 tcgcgaaggt cggcgacgac ctggttgttc tgcgacctcg ctatacgagg tattcggcgg
     2341 tgttgcctgc cggcgactga gcggcgttta gccgttccga cactcgggaa ccccttgact
     2401 gcgcagggaa ttacctctgt gtggcccagg agatgggcag aacccatggc tgattcccct
     2461 aacggaaggt tgagcatgac cgagaagaac agtggtcccc aagaggccgt caagggcgcc
     2521 gtcgaagacg tcaagggcaa ggctaaggaa gccgtcggca ccgtgaccgg tcgcaacgac
     2581 ctggtcgacg agggcaaggc ccagcaggac aaggccgacg cccagcagaa tgcggccaag
     2641 aaggaagccg aagccgacgc agcccggggt agcgccaagg ccgccgagaa gcgccaagag
     2701 agcaaccagt aggacttagc ttcacagcaa aaggtcccag ccgttgaacc ggctgggatc
     2761 ttttgtgttc ggcggtctac tagctgctgg gtcgttgccg gtgcgcttcg ggatgttctg
     2821 cgtaccagcg ctgttcgata ccgcgcaccc gccgatgctc gacgtacagc cacagcaggc
     2881 cgacgatgag gaaggccgcc gagagcgacc cgaataccag gccggtgctg tggtggttcg
     2941 acgcatgcgc ggccaggcct ccaacgaaac atgccagcgc cacgccgatg acgatcagcg
     3001 cgggcatgtt cttggtgtcg gtcatcgact cgccggcgtg cggccgggtc gtcctcgcat
     3061 ggtcgacggg atctttagga ctcttcatgg gtgctccttt cgccacacct ctgcccgttt
     3121 tccagggata tagccacggt agaaccggcg actccacccg acggcgaatt ccccatcact
     3181 gctggggcgg gtattggtgg gcccggagca gaccggcaag gtgtgcggca ttgcgtgccg
     3241 cggaagccgt cgtcttggcc accggctcgg ggacctcgtc gaggtcgttg tagttggtgg
     3301 tctgcattgc ttcgccgttc caatacgtgt tcgcctgagc cgcgaacgaa aagccgacgt
     3361 cgttgagccc ctgggcgcag tcggcgaaaa ccttgtgcgc gccgtcttcg ttgccgacga
     3421 ccgagacgat ggcgactttg ccgtacatga tcggccggcc ctcgtcgtcg gtattggaca
     3481 gctcggcgtc gaggcgttcc agcacgcgct gggtgatgct ggacgggtgg cccagccaga
     3541 cgggcgtcga cagcagcagg atgtcggcgt cgaggacgcg ctgcctgatc ttgggccact
     3601 cgtcgccgtc gcccatgtcg gcctcgacgc cgggggcgat cgaatagtcg gcgcatcgca
     3661 gactgtcggt tttgaccccg tggctgcgca attgttggca gacatgttcg gccatcagtt
     3721 cactgctcga ttcggccggg ctgcgcttca ggctgcacac gagtgcaacg gcagtgaggg
     3781 gacgttctga attgtccggc attgcattgt ctttcggtca gggcatggcg atggatggca
     3841 cgctcccaac gagggccaac agcatcgcgg ccatcaggaa ccaacccgcc ccctgccacg
     3901 gccacgtgat gccgttctgc cgccagattc gataggtccg gacgaacgct cgaactccgc
     3961 cgtacaacaa gatcagcggt gcgccgaggg cgagaagggt acgttgcggt gcaccacagg
     4021 cagccgtgtc gaaggtcgcg ccgccacagg tgctgaccca gacggttgcg acaaccagga
     4081 acacgaccgc agccacggcc acaccgacgc tgaaccggac agcgtcgaat acaccgtcgc
     4141 ggccgcgggc ttcgtcgtgg cgcaagtctt cggtactgcg gtacacggat agctaattcc
     4201 cctggttaca gagggataaa ccttccgtat cgatgacttg gacgacggcc tgtcgtcggt
     4261 tcgccgcgac catgggctgt agctatgccc ggcagtcacc gccccatacg agttcgcgcg
     4321 cctcagaagg gtggtggttg gtcgttggcg gcgagccagg cttgtcgttc tcgttgttct
     4381 tgtgcgttgc gatcggtgcg ttcgcggcgt ttattggtga tgcggtcgcg gcggccttgt
     4441 tcgcgggtct gcctgcgtgt gggcatcatc atcgcgcggt ggggggatgg gtcgggcgcg
     4501 gtgatgtcac ccaaggtggg cggtgggttg ggccagcgcg ggaaacaggg tgccgccgtg
     4561 gggatcggtg ctgtagctat gcccggtcgg ggggtccagg tgatggtgcc gtcggcgtgc
     4621 tggcgttcgg tccacgcgaa gccggcgtag aacgtcttga tcaggtgatg gcaacgaaat
     4681 gccgaaggac gttctggtgg cgaccgctga gttgtcaaca tctgctagcc cttgccggtc
     4741 aatccaacgg tgacatttgc tccggcgggt taaatgaaca cgctcgggcc gcgaccttga
     4801 gcgcaatgag cacaccgtga gtgcggtagt acagcttggt gttcgacgga tgcgtcggcc
     4861 cgtagggata gggcgcggtg tggatgctcc aatgcagtgt caagtgccat ttcaggtcga
     4921 agtagctaga tcggcctgga gtgctcgata gagctcgcgg actactgctc gttgaggcag
     4981 cggatgatct ccttcttggt cttaacttct tgtgttcggc gggccacgta ttgctttgtc
     5041 ggttagcgcc gcatccgaag aagcctcgaa cacaccttcg gcgctactta gcatctccga
     5101 cacgccgagc gagttatcca caagccctgg tttgtcgggc cacggaccta ccattcgcct
     5161 cgtgatcccc accagggcca aggtgtacgc ggccgtcggt gtcatatcgg gagtggccgc
     5221 tcatctcagc ggcatcagcg cgcctgtgag cgtggccgtc ggactcgtcg cgccggttct
     5281 cctcgccgcc gcgccccggt tcctgctggc caccgtcagg ggcgccttca cggcgagccc
     5341 acgggaggcg agcccgacgt cggcaatgtc gggcatggag ttcgaagacc acgtcgcgcg
     5401 gatcgcgagg tcgtgcggag tcccggtgat catgacggcc gtgaccggcg actggggcgt
     5461 cgaccttgtg ctcggacagc ggcccgaacg ccttgccgtg caatgcaaac ggcaatcacg
     5521 accggtcggc gcaggcgccg tccaggaggt cgtcgcgggc gcgccgatgc acggctgcac
     5581 gaaaaccatg gtcgtcacca accacgaatt cacacgggca gcaatcaaac tcgcggagtt
     5641 acacggctgc gagcttgtcg gcggatcgga actgccgcga ctgcgatcga tcctccgacg
     5701 gcttacggag ccgtcggccc cgacggcacc ctagccgagc gcttcacgca ccgccgcaac
     5761 cgcctcgtcg atctcggcgt acgacacgat cagcgccggg cggaaccgga cactgtccgc
     5821 cccgctggcg agcatcacga cctggcgatc ccagagtttg cggatcaggt cgtcacgctg
     5881 ttctcgcgac gggaggctga acgcccgcat caaccctcgg ccgcggacgt cgcgcaccga
     5941 ctcggggaac tcggcagcca actcctccag ccgggcgtgc agatagcggc ccgcctcggc
     6001 ggcgtgctcc atcagaccgt cggcttcgat gacctcgagg atgcggcggg agcgaaccat
     6061 gtccaccaga ttgccgcccc aggtcgagtt gatccgcgag ctgaccgcaa acacgttgtc
     6121 ggcgacctcg tcgacgcggc gccccgccat cacaccgcac acctgcgtct tcttgccgaa
     6181 cgcgaccacg tcgggcgcga cgcccaattg ctgataggcc catgcggttc cggtcagccc
     6241 gcagccggtc tgtacctcgt cgaagatgag cagcgcgtcg aactcgtcgc acagctcgcg
     6301 catcgccgcg aagaactgcg gacggaaatg ccggtcaccg ccctcaccct ggatcggctc
     6361 ggcgatgaag caggcgatgt catgcgggtg agcttcgaaa gctgcccgcg cctgtcgcag
     6421 cgactcggcc tccacggcgt ccatgtcggc atcgggtcgg atgtacggag cgtcgatgcg
     6481 cggccagtcg aacttcggga accgcgcgac cttgttcggg tcggtgttcg tcagcgacag
     6541 cgtgtacccg ctgcggccgt ggaacgcgcc gcgcagatgc agaaccttgg tgccgagttc
     6601 ggagcccgca cgcgaggagg acgaagaaga ggctgtgccg cgtgcgtcgt tgagcctgct
     6661 cttccagtcg aacgccacct tcagcgcgtt ctctaccgcc agcgcacctc cgtcgacgaa
     6721 gaacaagtgc ggcagcgcgg ggtcaccgag cacacgggcg aaggtgtcga cgaagcgcgc
     6781 catcgggacg ctgtacacgt cagagttgct cggcttgttc accgccgcac tcgcgagctc
     6841 ggcgcggaac gcctcgtcgt cggccagcgc cggatggttc atgcccagcg ccgacgacgc
     6901 aaagaacgtg aacatgtcga ggtagcggtc gccggtgcgg gcgtcgacca ggatcgagcc
     6961 ggtcgagcgc tcgatgtcga gcacgaagtc cagaccgtca gcgagaatgc tgcggctcag
     7021 cacctcgcgc acgtcggcgg ggccg
//
LOCUS       DASYLX010000893         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1590832,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000893 DASYLX010000000
VERSION     DASYLX010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590832"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGP27_20880"
     CDS             complement(<1..345)
                     /locus_tag="VGP27_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HEV7853787.1"
                     /translation="MTSTDHRPQDLRQQAQRHAEEARAKMADRRAEQDGGLSGWLAER
                     AGEWDLRGPDEATMQRQKYLWNFLVDYWFRMEMDGWENLPEPPVLLVGIHSGAPFVWD
                     AWTVGVQWWRRFG"
     gene            complement(365..2119)
                     /locus_tag="VGP27_20885"
     CDS             complement(365..2119)
                     /locus_tag="VGP27_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853788.1"
                     /translation="MTTEIEMTKTPVVANADVAVAETARRRRRMDHDHPFYKWVVLSN
                     TTLGTLLATINASIVLISLPAIFRGIGLNPLAPGNVSYLLWMLMGYLVVTAVLVVPFG
                     RLGDMFGRVRIYNVGFVVFTFAAVALSFDPFHLGGGALWLIAWRVVQGIGGAMLMASS
                     SAILTEAFPSNQRGMALGVNMVAAVAGSFLGLLIGGFLSEWHWKAIFWVGVPIGILGT
                     IWSIRSLRELGVRTPGRLDWKGILSFGVGLTVLLTGITYGIQPYGVSTTGWTNPWVLG
                     AIAFGLLSLVGFCFIESRVADPMVDIRLFRSASFGMGNLAGLMSSMGRGGLQFMLIIW
                     LQGIWLPLHGYSFESTPLWAGIYMLPMTAGFLVAGPLAGSLSDRFGARPFTVGGMLLM
                     AATFVALVMIPVNFDYWLFAVLVFLNGLGGGIFTAPNTAAIMSSVPAAQRGAASGVRA
                     TFFNAGSSLSIGVFFSLMIIGLAHTLPTAMSSGLQAQGVPASVAHDVANLPPVGSLFA
                     AFLGYNPIAELLAPSGALHAPGVDAGVLTGKTFFPQLITEPFHTGLVVVFVAAAVMML
                     IGAVASMFNPGRYGTADD"
     regulatory      2221..2332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2322..>2642
                     /locus_tag="VGP27_20890"
     CDS             2322..>2642
                     /locus_tag="VGP27_20890"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV7853789.1"
                     /translation="MNFLPLTPGGQTPPRVMTIAGSDSGGGAGIQADMRTFALLGVHA
                     CVAVTAVTVQNSMGVKGFHEIPVDVIAGQIEAVASDIGIQAAKTGMLASSDIIGTVAD
                     TWRSQ"
BASE COUNT          523 a          872 c          863 g          384 t
ORIGIN      
        1 gccgaagcga cgccaccact ggacgccgac cgtccatgcg tcccagacga acggtgcgcc
       61 cgagtggatg cccacgagca gcaccggcgg ttccgggaga ttctcccagc cgtccatctc
      121 catgcggaac cagtagtcga cgaggaagtt ccacaggtac ttctggcgct gcatcgtcgc
      181 ctcgtcggga ccccgcagat cccactcgcc cgcgcgttcg gccagccagc cgctgaggcc
      241 gccgtcctgc tcggcgcgtc ggtcggccat cttggcgcgg gcctcttcgg cgtggcgctg
      301 tgcctgctgt cgaagatcct gaggccggtg atcggtgctt gtcatgcgca ccgagtaccc
      361 attttcagtc gtcggcggtg ccgtaccggc cggggttgaa catcgaggcg acggcaccga
      421 tgagcatcat caccgcggcc gcgacgaaca ccaccaccag cccggtgtgg aacggctcgg
      481 tgatcagctg cgggaagaac gtcttaccgg tcaacacgcc ggcgtcgacg ccgggggcat
      541 gcaacgcccc cgacggtgcc agtagctcgg cgatggggtt gtagcccaga aacgccgcga
      601 acagactgcc aacgggaggc aggttggcca cgtcgtgtgc gacggatgcg ggcaccccct
      661 gcgcttgcag gccactgctc atcgccgtcg gcagagtgtg cgccagcccg atgatcatca
      721 gcgagaagaa gacgccgatc gacagcgacg aacccgcgtt gaagaacgtc gcgcgcacgc
      781 cagaggccgc accgcgctgg gcggcgggca cgctggacat gatcgccgcg gtgttgggtg
      841 ccgtgaagat gccgccgccg agaccgttga ggaacaccag caccgcgaaa agccagtagt
      901 cgaagttcac cggaatcatc accagcgcga cgaaggtcgc cgccatcagc agcattccgc
      961 ccaccgtgaa cgggcgcgcc ccgaagcggt cggacagcga tcctgccagg ggtccggcca
     1021 ccaggaagcc cgccgtcatc ggcagcatgt agatgccggc ccacagcggt gtcgactcaa
     1081 agctgtagcc gtgcaacgga agccagatgc cctgcagcca gatgatcaac atgaactgca
     1141 ggccgccgcg tcccatcgac gacatcaggc ccgcgaggtt gcccatgccg aacgaggcgg
     1201 agcggaacag ccggatgtcg accattgggt cggccacccg cgactcgatg aagcagaacc
     1261 ccaccaggga cagaagtccg aacgcgatcg cccccagcac ccacgggttg gtccaaccgg
     1321 tggttgagac gccgtagggc tggatgccgt aggtgatgcc ggtgagcagc accgtcaggc
     1381 cgacgccgaa ggacaggatg cccttccagt ccagccggcc cggcgtgcgg acgccgagtt
     1441 ccctcagcga ccggatgctc cagatcgtgc ccaggatgcc gatcggcacg ccgacccaga
     1501 agatcgcctt ccaatgccac tcggaaagga agccgccgat gagcaggccg aggaacgatc
     1561 cggcgacggc ggccaccatg ttcacgccga gcgccatacc gcgttggttg gacgggaacg
     1621 cttcggtgag aatcgccgac gacgacgcca tcagcatcgc gccgccgata ccctggacca
     1681 cgcgccaggc gatcagccac agcgcaccgc cgccgaggtg aaacgggtcg aacgacagcg
     1741 cgacggccgc gaacgtgaac accacgaagc cgacgttgta gatgcgcacc cggccgaaca
     1801 tgtcgcccag ccggccgaac gggaccacga gaacggctgt gacgaccaga tagcccatca
     1861 gcatccacag cagatagctg acgttgccgg gcgccagcgg gttgaggccg atgccgcgga
     1921 agatcgcagg cagcgagatg agaacgatcg acgcgttgat ggtggccagc agcgtgccca
     1981 gggtggtatt ggacagcacc acccacttgt agaagggatg gtcgtggtcc attcgcctgc
     2041 gccggcgggc ggtttcggcg actgccacgt cggcgttcgc cacgactggg gttttcgtca
     2101 tctcgatctc ggtcgtcatc aacttcgctt acgtatcaaa tcggctgcaa aacacatatg
     2161 ttagctatac aaaccaaccg ggagcaattt gtgttccatg cgatagcgtg gagacgccct
     2221 gatcaaccac gcgggagcgc gtacagagcg cgctgagagg acggctgagc tgccgtcgac
     2281 cgtacgaacc tgaccgggta atgccggcgt agggagtgct agtgaatttc ctgcctctga
     2341 ctcctggcgg tcagacccca ccgcgggtga tgacgatcgc cggctccgac tccgggggcg
     2401 gcgccggtat ccaggccgac atgcgcacgt tcgcgttgct tggcgtgcat gcctgtgtcg
     2461 ccgtgaccgc ggtgaccgtg cagaactcga tgggcgtcaa aggctttcac gagatccccg
     2521 tcgacgtcat cgcgggccag atcgaagccg tggcgagcga tatcggaata caggcggcca
     2581 agaccggcat gctggcgtcg tccgacatca tcggaacggt cgccgacacc tggcgctcac
     2641 ag
//
LOCUS       DASYLX010000894         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2575987,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000894 DASYLX010000000
VERSION     DASYLX010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575987"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1291
                     /gene="mshC"
                     /locus_tag="VGP27_20895"
     CDS             53..1291
                     /gene="mshC"
                     /locus_tag="VGP27_20895"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEV7853790.1"
                     /translation="MQSWQAPSVPVLAGRGPQLRLYDTADRQVRPVSAGETASMYVCG
                     ITPYDATHLGHAATYLTFDLVHRLWLDANLRVNYVQNITDVDDPLFERAARDGIDWRE
                     LADRETALFSEDMTALRVIPPLAYVAATEAIGEVVELVEKMLASGAAYVVDDAQHPDV
                     YFRADATAQFGYESGYDRDTMLRLFGERGGDPGRPGKSDELDALLWRAERPGEPSWPS
                     PFGPGRPGWHVECAAIALNTIGTGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARH
                     YVHAGMISWDGHKMSKSRGNLVLVSGLRADGVDPGAIRLGLLAGHYRSDRSWSDAVLA
                     EALARLQRWRDATALQAGPDATDVIASVRRYLADDLDTPKALAALDGWATDALTYGGH
                     DTEAPRTVAAAVDALLGIKL"
     gene            1316..2194
                     /locus_tag="VGP27_20900"
     CDS             1316..2194
                     /locus_tag="VGP27_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853791.1"
                     /translation="MSAVNKKVVLITGGARGVGAEVARRLHEKGADLVLTDVDEAALA
                     DIGEELGGDRLLTAVADVRELAAMEQVASRAVDRFGGIDVVMANAGIATYGSILKVDP
                     EAFRTLIDVNVVGVFNTVRAALPSVMDRRGYVLIVSSAAAFAASPGLAPYNAAKAAVE
                     QFANALRLEVAHRGVDVGSAHMLWIDTPMVRDAKSDLPSFGKMLDALPGPLGRTTSVE
                     ACGKAFVKGIEARKRYVYCPDYVSVMRWLKPLLSTRLGESTTLKSVPELLPQMDAEVE
                     ALGRSMGARTKKVEKK"
     gene            2191..>2411
                     /locus_tag="VGP27_20905"
     CDS             2191..>2411
                     /locus_tag="VGP27_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope protein"
                     /protein_id="HEV7853792.1"
                     /translation="MIRRIGVVIAVLVVLAACSGKKADEADKSPAPPSPAAVTPEAAR
                     AIAKEAYIYGFPMVDSYRVQYSYFVNNQDP"
BASE COUNT          385 a          788 c          847 g          391 t
ORIGIN      
        1 atccggtcgg ccgtcagggg cggtccccgg cggaccttct agagtcgaag ccatgcagtc
       61 gtggcaggcg ccgagcgttc cggtcttggc ggggcgcggt ccgcagctgc ggctgtacga
      121 caccgccgac cggcaggtga ggccggtgtc cgcgggcgaa acggcaagca tgtacgtctg
      181 cggtatcacc ccgtacgacg ccactcatct gggccatgcc gcgacctacc tgacgttcga
      241 tctcgtgcac cggctgtggc tcgatgcgaa ccttcgggtg aactacgtgc agaacatcac
      301 cgacgtcgac gatccgctgt tcgagcgggc cgcgcgcgac ggcatcgact ggcgggagct
      361 ggccgaccgg gagactgcgc tgttctcaga ggacatgacg gcgttgcggg tcatcccgcc
      421 gctcgcctac gtggccgcga ccgaggcaat cggcgaggtc gtcgagctgg tcgagaagat
      481 gcttgcctcc ggcgcggcct acgtcgtcga cgatgcgcag caccccgacg tttacttccg
      541 ggccgacgcc accgcgcagt tcggctacga gtcgggctat gaccgggaca ccatgctccg
      601 gctgttcggt gagcggggcg gtgaccccgg ccgtccgggc aagagcgacg aactcgatgc
      661 tctgctgtgg cgggccgagc ggccgggcga gccgagctgg ccgtcgccgt tcggtcccgg
      721 caggcccggc tggcatgtgg aatgcgccgc gatcgcgctc aacacgatcg gcaccggcct
      781 cgacatccag ggtggcggca gcgacctgat cttcccgcac cacgagtttt ccgccgcgca
      841 tgccgaatcc gtcaccggcg agcggcgatt cgcccggcac tacgtgcacg cgggaatgat
      901 cagctgggac ggccacaaga tgagcaagag ccgcgggaac ctggtgctgg tttccggttt
      961 gcgcgccgac ggcgtcgatc ccggcgccat ccggctcggg ttgctggccg ggcactaccg
     1021 ctcggaccgg tcgtggagtg acgcggtgct ggcggaggcg ctcgcccggc tgcagcggtg
     1081 gcgtgacgcc acggcgctgc aggcaggccc ggacgccacc gacgttatcg ccagcgtgcg
     1141 ccgctatctg gccgacgacc tcgacacccc gaaagccctt gccgcactgg atggctgggc
     1201 cacggatgcg cttacttatg gcggtcacga caccgaagcg ccgcgaacgg tggccgcggc
     1261 cgtcgacgcg ttgctgggca tcaagctgta gaacgctttg cgaaagggat cacgcatgag
     1321 tgcggtgaac aagaaggtag tcctgatcac gggcggcgcc cgcggggtgg gcgcggaagt
     1381 cgcgcgccgg ctgcacgaga agggcgccga cttggtgctg accgacgtcg acgaggccgc
     1441 gttggctgac atcggcgagg agttgggcgg ggaccggctg ctcaccgccg tcgccgatgt
     1501 ccgcgaactg gcggcgatgg aacaggtggc gtcccgcgcg gtcgatcgat tcggcggtat
     1561 agacgtcgtg atggccaatg ccggcatcgc gacctacggt tcgatcctca aggtcgatcc
     1621 cgaagcgttt cgcacattga tcgacgtcaa cgtcgtcggc gttttcaaca ccgttcgcgc
     1681 cgccctcccg tcggtgatgg acaggcgggg ctacgtgttg atcgtgtcgt ctgcggcggc
     1741 cttcgcggcg tcgcccgggc tggctcccta caacgctgcc aaggcggccg tcgaacagtt
     1801 cgccaatgcg ctgcggttgg aggtggcgca ccgcggtgtc gacgtcgggt ccgcgcacat
     1861 gctgtggatc gacaccccga tggtgcggga cgccaaatcc gatctgccga gtttcggaaa
     1921 gatgctcgat gcgctgcccg gcccgctcgg caggacgacc tcggtcgagg cgtgcggcaa
     1981 ggcattcgtc aaaggcatcg aagcacgcaa gcgctatgtc tactgtcccg actacgtttc
     2041 ggtgatgcgc tggctcaagc cgctgctctc cacgcggctc ggcgagtcca ccactttgaa
     2101 gtccgttccc gaactgctgc cacagatgga cgccgaggta gaggcgctcg gccgatccat
     2161 gggcgcccgc accaagaagg tcgagaagaa gtgattcgcc gcatcggtgt agtgatcgcc
     2221 gtcctcgtcg tgttggcggc ctgcagcggc aagaaggccg acgaggcgga caagtcgccg
     2281 gcgccgccaa gcccggccgc tgtgacgccg gaggcggccc gtgccatcgc gaaggaggcc
     2341 tacatctacg gcttcccgat ggtcgacagc taccgggtgc agtacagcta cttcgtcaac
     2401 aaccaggatc c
//
LOCUS       DASYLX010000895         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3345114,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000895 DASYLX010000000
VERSION     DASYLX010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345114"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGP27_20910"
     CDS             complement(<1..435)
                     /locus_tag="VGP27_20910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7853793.1"
                     /translation="MPNSRSVAAQLNPVSGSLALRAAFGCFPSGVIAVCALEGDGIPV
                     GIAASAFVSLSLDPPLVAISIQRTSTSWPRLRQSERLGLSVLGHDQEHACARLSGKGD
                     RFGAIDWMPTGTGAVMIGGASAWLECALFDEVPAGDHTLAILQ"
     gene            572..796
                     /locus_tag="VGP27_20915"
     CDS             572..796
                     /locus_tag="VGP27_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7853794.1"
                     /translation="MAVTQPTLLVTVDRAKCCGYTLCAAEGPDVYSIDDAGYAVAMDG
                     VPADLEAQACRGAEACPDGAITVRRIGDGE"
     gene            complement(827..1405)
                     /locus_tag="VGP27_20920"
     CDS             complement(827..1405)
                     /locus_tag="VGP27_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853795.1"
                     /translation="MAANGETLARIFDATYTTLARSEARGLSMTDVSAQAGISRGALY
                     HYFNSKQDLLHAIGVKVEKLFEDAVIAAVEERPELDVRVRVVVEAMTDVGRSHPEVVQ
                     VLALEPAFGVDFLRQIFPDFVAVLEELLAPALVTSRAVRSMGLTSGQLCELILRITMS
                     AYVLPTRDLSDVSRAILALPCLAESTPDLYLV"
     gene            1779..2894
                     /locus_tag="VGP27_20925"
     CDS             1779..2894
                     /locus_tag="VGP27_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7853796.1"
                     /translation="MTTTQESAADLAEFDKKLDRLHLRGQWQYDAQLIQLTDGPVPAG
                     VPFRWSRELADRALAEMCTAVSGETARRNFTFINPAPDVNGTTHTLAMGMQITLPGEI
                     AWAHRHSINALRFVVDGSPDLYTAVDGERLAMEDGDLIITPAYTWHDHHNESAKRGVW
                     VDILDVPLMGYLRQLFFEPFGGSTQPLHDNAADHISSRASNIRPAWEARQLSRIPFRY
                     AWRDVRAALDTHRHNSGSAFDGVILHYAHPVTGGPTLPTIDCFAQMLRPGLHTERHRH
                     TSSAVYYVVEGEGTTLVGDEELEWSVGDSFVVPNWMWHAHVNRSGDTPAVLFSATDAP
                     VLEALGMYREEPYPSFGIAPGPIVPGDRARRGSAERT"
     gene            2896..>3726
                     /locus_tag="VGP27_20930"
     CDS             2896..>3726
                     /locus_tag="VGP27_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7853797.1"
                     /translation="MTISRADIRGVVGIVPTPATRDAGDWRCVNSVNVAETENMIEQV
                     VDAGIELVMATGTFGECASLTHEEWLLFSRCVADTVRGRVPFFAGVTTLNTRDTIARG
                     HEAIAAGADGLFVGRPMWLPLDDDGIVRFYRDITEALPRVPVVIYDNPAAFKGKISVA
                     AYLQLADLPEIVATKHAGGPSLESDIEAFGDKVAVLPLDSDWVRAAKRFPDRATACWS
                     GNVACAPSPLAALSRAVLSQDWAGADHISEQVNWALAPQFGGDMSRFMDYSIPVGRGR
                     F"
BASE COUNT          699 a         1223 c         1171 g          633 t
ORIGIN      
        1 ctgcaaaatg gcgagcgtgt ggtcgccggc ggggacctcg tcgaacagcg cgcactccaa
       61 ccacgcggac gccccgccga tcatcaccgc ccctgtgccg gttggcatcc agtcgatcgc
      121 gccgaagcgg tctcccttgc ctgacaacct tgcgcaggca tgttcctgat catgcccaag
      181 cacgctaagt ccgaggcgtt cgctctgccg taatcgtggc caggacgtgg acgtccgctg
      241 gatggagatc gccaccagcg gtggatcgag cgatagtgag acgaatgcac ttgccgcgat
      301 gccgaccggt atgccgtctc cttcgagcgc acagacggcg atgacaccac tggggaagca
      361 cccgaacgcc gcacgcagcg ccagcgagcc ggataccgga ttgagctgcg ccgcaacgga
      421 tctagagtta ggcatgatgc gccccgatgg atcgccccgc atcgctcagg aatgagacca
      481 tatatgcaaa taaaacatta tctggatatc ctgtctacaa tcgaggacat accgcagcca
      541 tcgcacacca gggtcaccag aggagccgat catggcagtc actcagccga cacttctcgt
      601 caccgtcgat cgcgcgaagt gctgcggata caccctgtgc gccgccgaag ggcccgacgt
      661 ttactcgatc gatgacgcgg gatacgccgt ggcaatggac ggggtgcccg ccgatctgga
      721 ggcgcaggcc tgccgcggcg cggaggcctg tccggatggc gccatcaccg ttcggcgcat
      781 cggtgacggc gagtagcggc acagagccgg ccgatcagta ggaacttcag accaggtaca
      841 ggtcgggagt cgactcggcc aggcagggca atgccaatat cgcgcgagaa acatcggaca
      901 ggtcgcgagt cggcagcacg taggcggaca tcgtgatgcg aaggatcaac tcacagagct
      961 ggccgctggt gagccccatg ctgcgaaccg ccctgctcgt caccagcgcc ggagccagca
     1021 gctcctcgag cacggcgacg aagtcgggaa agatctgccg gaggaagtcc acgccgaagg
     1081 cgggttcgag ggcgagcacc tgtaccacct cgggatgcga gcgtccgacg tcggtcatcg
     1141 cctcgacgac cacgcgcacc cgaacgtcga gttcgggccg ctcctcgacg gccgcaatga
     1201 cagcgtcttc gaagagcttc tcgaccttca ccccgatggc atggagcaga tcctgcttgc
     1261 tgttgaagta gtggtacagc gctccgcggg agattcccgc ctgggcgctg acatcagtca
     1321 tcgacagccc ccgggcttcg ctgcgtgcca gcgtcgtata cgttgcgtcg aagatccgtg
     1381 cgagggtctc accgttcgcc gccattggcc ctcctcgtcg ttcccgctct gcagagtgta
     1441 ttcccgctgt gcagagaaaa gaacacaaac gacaacaatg ctgtgatctg ttcaagcgga
     1501 aatgtctgca ccatgacgga ccgatcgagt gcacccttgg cttcgccggg tgccgggccg
     1561 ccgataaagg tattgaatag acaaaatcaa tactaagtgt cttgtagtcc gcacaagccg
     1621 gtgctagcgt cagcgctgcg tcgtcatcgt cggcagctgt ctctccgatc cagggatcga
     1681 cgctcgccgc caccctgggc ccttcgtcga ccggcgacgg cgcgaagtac gacgggcttc
     1741 cagagaaagc cagcggatcc ttcacgagga caagggatat gacgacgaca caggagtccg
     1801 ctgcagatct ggcggagttc gacaagaagc tcgaccgact ccaccttcgg ggccagtggc
     1861 agtacgacgc gcagctgatc caactcaccg acggtcccgt cccggccggc gtgcccttcc
     1921 ggtggtcacg ggaactcgct gatcgcgcgt tggccgagat gtgcaccgcg gtatccggtg
     1981 agaccgccag gaggaacttc accttcatca atccggcgcc cgatgtgaac ggcacgacac
     2041 acaccctcgc aatgggcatg cagatcaccc tgcccggtga gatcgcctgg gcgcaccggc
     2101 actccatcaa tgcactgcgc ttcgtcgtgg acggaagtcc cgacctctac acggctgtcg
     2161 acggtgagcg gctcgcgatg gaggacggtg acctgatcat caccccggcc tacacctggc
     2221 acgatcacca caacgagagc gccaagcgag gcgtctgggt cgatatcctc gacgtgccgc
     2281 tgatgggcta tctgcgccag ctgttcttcg aaccgttcgg cggtagcacc cagccattgc
     2341 acgacaacgc ggccgaccac atcagcagcc gcgcctcaaa catccggcct gcctgggaag
     2401 cacgccagct ttcccgcatt ccgttccgtt atgcctggcg cgatgtccgg gccgccctgg
     2461 acacccaccg gcacaactcg ggcagtgcgt tcgacggcgt catcctgcac tatgcccatc
     2521 cggtcaccgg cggccccaca ctgcccacca tcgactgctt cgcgcagatg cttcgtcctg
     2581 gtctgcatac ggagcgccac aggcacacgt ccagcgcggt gtactacgtc gtcgagggcg
     2641 agggcacgac actcgtcggc gacgaggagc tcgagtggtc ggtcggcgat tcgttcgtgg
     2701 tgccgaactg gatgtggcat gcccacgtca accggtccgg cgatacgccg gccgtgcttt
     2761 tctccgctac cgacgccccg gtgctggaag cgctcgggat gtaccgcgag gagccttatc
     2821 cctcgttcgg tatcgctccc ggtcccatcg tgccgggcga tcgggcccgg cgcggttcgg
     2881 cagagaggac atgacatgac gatcagccgt gccgacatca ggggtgtcgt cgggatcgta
     2941 cccacaccgg cgaccaggga cgccggtgat tggcggtgcg tgaacagcgt caacgtcgcc
     3001 gagaccgaaa acatgatcga gcaggtcgtc gacgccggaa tcgaactggt gatggcgacc
     3061 ggcacgttcg gtgagtgcgc gtcgctgaca catgaagagt ggcttctgtt ttcgcggtgt
     3121 gtcgccgaca ctgtccgggg tcgggtgccc ttcttcgccg gcgtgacgac actcaacacg
     3181 cgcgacacca tcgccagggg acacgaagcg atcgccgccg gtgcggacgg gctgttcgtc
     3241 ggtcgtccaa tgtggctccc actcgacgac gacggcatcg tgcgcttcta ccgcgacatc
     3301 accgaggcct tgccgcgggt acccgtggtg atctatgaca acccggctgc attcaagggc
     3361 aagatcagcg tagcggccta tctgcaactc gcggatcttc ccgagatcgt ggcgacgaag
     3421 cacgccggag gcccgtcgct ggagagcgac atcgaggcgt tcggcgacaa agtcgccgta
     3481 ctccctctcg acagcgactg ggtcagggcc gccaagcgct tccctgaccg ggccaccgcg
     3541 tgctggtcgg ggaacgtggc gtgtgccccg tcgccgctgg ccgccctgtc ccgcgcggtg
     3601 ctgtcccagg actgggccgg cgcggatcac atttccgaac aggtgaattg ggcgttggca
     3661 cctcagttcg gcggcgacat gtcgcgtttc atggactaca gcatcccggt cggtcgcggc
     3721 cggttc
//
LOCUS       DASYLX010000896         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3785631,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000896 DASYLX010000000
VERSION     DASYLX010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785631"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGP27_20935"
     CDS             complement(<1..920)
                     /locus_tag="VGP27_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV7853798.1"
                     /translation="MRAGIVVSVTLQDRLRLEAIVADRNTRQKHAARARVIVATADGC
                     GTTEIMRRSGLSKPVVWRWQERFMREGIDGLLRDKTRRPGKPPLPAETVQRVLDLTLA
                     DPPGEATHWSVRAMAKVAGIGAVSVQRIWQANGLTPHRMKTFKLSNDPKFAEKFSDVV
                     GLYVDPPAHAVVLSIDEKSQIQALDRTQPGLPMKKGRAGTMTHDYIRHGTTTLFAALN
                     VLDGTVIGRNMQRHRHQEFIRFLNQVEAAVPAGKTVHAVVDNYATHKHPKVRAWLVRH
                     PRWTFHFTPTSASWLNAVEGFFSALTRRRL"
     gene            1001..>1807
                     /gene="rsgA"
                     /locus_tag="VGP27_20940"
     CDS             1001..>1807
                     /gene="rsgA"
                     /locus_tag="VGP27_20940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV7853799.1"
                     /translation="MPLLIEQYGWSDALRLAFEPHARAGYLPGRIIVQQREANVVVTD
                     AGNLSARLSGRLRHEAREGGHPAAGDWVALSANAGESKATLHAVLPRRTAFVRRAADS
                     VQTLQVIAANIDVVFIVTSMNADLNPRRLERFLAAAWQSGARPVVVLTKADLCEHPGD
                     QAAEIAKLAAGCPVLIVSAREGLGLGGLMNHIAPGETCVLIGSSGVGKSTLVNALLGE
                     ARMATQDIRAGDARGRHTTSYRHLVLLPGGGLILDTPGIREVGLIDADAGL"
BASE COUNT          279 a          581 c          630 g          317 t
ORIGIN      
        1 ttgagccggc ggcgggtgag cgccgagaag aagccctcca cggcgttgag ccaggatgcg
       61 ctggtgggcg tgaagtggaa ggtccagcgt gggtggcgga cgagccaggc gcgcaccttg
      121 ggatgcttgt gggtggcgta gttgtcgacc acggcgtgaa ccgtcttgcc ggccggcacc
      181 gcggcctcga cctggttgag gaaacggatg aactcctgat gccggtgccg ctgcatgttg
      241 cggccgatca ccgtgccgtc cagcacgttg agcgcggcga acagcgtcgt cgtgccgtgc
      301 cggatgtagt cgtgggtcat cgtcccggcc cggcccttct tcatcggaag gccgggttgg
      361 gtgcggtcga gcgcctggat ctggctcttc tcgtcgatcg acagcacgac ggcatgggcg
      421 ggcggatcga cgtagaggcc cacgacatcg ctgaacttct cagcaaactt tgggtcgttg
      481 gagagcttga acgtcttcat ccggtgtggc gtcaggccgt tggcctgcca gatgcgctgc
      541 acgctgacgg cgccgatgcc ggccaccttg gccatcgcgc ggacgctcca gtgcgtagcc
      601 tcgccgggcg ggtcggccag cgtcaggtcg agcacgcgtt ggacagtctc agcgggcagc
      661 ggcggcttgc cgggcctgcg cgtcttgtcg cgcagcaggc catcgatgcc ttcccgcatg
      721 aagcgctcct gccaccgcca gaccaccggc ttggacaggc ccgagcggcg catgatctcg
      781 gtggtgccgc atccgtcggc ggtggcgaca atcacccgag cgcgggcggc gtgcttctgc
      841 cgggtgttgc gatcggcgac gatcgcttcg aggcgaaggc ggtcttgcag cgtgaccgag
      901 acgacgatgc ctgctctcat cccgagagct tgaacccatc tacccgttca gggaatcctc
      961 tgaatggggc aatccactag agtgcccatc ccctgtgaga gtgccgcttt tgatcgaaca
     1021 gtacggctgg tccgatgcgc tgcggctggc gttcgagccg catgcacgcg ccggctacct
     1081 ccctgggcgc atcatcgtcc aacagcgcga agcgaatgta gtggtcacgg atgccggcaa
     1141 cctaagcgcc agactgtccg gacgcctgcg tcacgaggct cgagaggggg gccatcccgc
     1201 cgccggcgac tgggtggctt tgtcggccaa cgccggcgaa agtaaagcca cactccatgc
     1261 cgtcctgcct cgccgcacgg ccttcgtgcg gcgagcggcc gacagcgtgc agacactgca
     1321 ggtcatcgcc gccaacatcg acgtcgtctt cattgtcacc tccatgaacg ccgatctcaa
     1381 tccgcgccgg ctcgagcgct tcctcgcggc tgcctggcag agcggtgcgc ggccggtggt
     1441 ggtgctgacc aaggccgacc tgtgcgagca ccccggggat caggccgccg aaatagccaa
     1501 gctggcggcg ggctgcccgg tgctcattgt ttcggcgcgc gagggacttg gcctgggcgg
     1561 cttgatgaat cacatcgcgc cgggcgagac ctgcgtgctg atcggctcat cgggcgtcgg
     1621 gaaatccacg ctggtgaatg ctctactggg tgaggcgcgg atggcgaccc aggacatccg
     1681 cgccggggat gcccgagggc gtcataccac cagctatcgc cacctcgtcc tgctgccagg
     1741 cggagggctg atcctcgaca cgcccggcat acgtgaagtc ggcctgattg acgctgatgc
     1801 gggtctc
//
LOCUS       DASYLX010000897         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2246423,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000897 DASYLX010000000
VERSION     DASYLX010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246423"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGP27_20945"
     CDS             <1..501
                     /locus_tag="VGP27_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7853800.1"
                     /translation="AQHWPQGNLSKAYAAYRTPFWRANGCSGEALSDEGPVFITFDVS
                     PGGDDNPGILLGFTDPRTFDPLPPAERRERALAGFAALFGDKALEPIDYVDHCWASEE
                     FAPGGPTAAVPPGSWTTYGPWLRKPVQGIFWAGTETADEWTGFLDGAVRSGRRAAAEV
                     HGELSG"
     gene            complement(498..1469)
                     /locus_tag="VGP27_20950"
     CDS             complement(498..1469)
                     /locus_tag="VGP27_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853801.1"
                     /translation="MPRGQRVNRTARRLAAADALPPSRPLNVRAGDGTALHAEVFGPE
                     DGYPIVLAHGITCALRVWAYQIADLACEYRVIAYDHRGHGRSGAPSRRANYGLNFLAA
                     DLDAVLEATLKPGERAVLAGHSMGGIAIASWSERYPRRVTERADAVALINTTTGDLLR
                     HVKLAAVPRPLTNARVRAAESLLRSFGAAPLLRAADRPSKRFVTMMAVGRDADPAVGE
                     FVYQLFTETPAASRGGWARKLIDSLGTHQHIGLQNLTVPTLVIGSRQDRLLPIVSSRH
                     IARTAPNLADIVELSGGHCAILEHPDEVNGHLRWLIESVAEMRRATS"
     gene            1533..2504
                     /locus_tag="VGP27_20955"
     CDS             1533..2504
                     /locus_tag="VGP27_20955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEV7853802.1"
                     /translation="MRAIQIPRLDGPAAAELVELEEPTDENAVIVEVHAAGVAFPDAL
                     QSRGLYQYKAELPFVPGAEIAGVVRSAPSGSGFAAGDRVAGLTMLNGGMAEVVALQPE
                     RVFKLPDNVSFEAGAGLLFNDLTMHHALRTRGRLTDGETVLVHGAAGGIGTSTLRLAP
                     VWGATRTIAVVGTEDKKAIAKSAGASDVVLADGFKDAVMGLTDGKGVDIVVDPVGGDR
                     FTDSLRCLAPGGRLLIIGFTGGEIPTIKANRLLLNNVDAVGVGWGAWTFTHPNYLREQ
                     WDDLEPLLASGAVSAPEPEIYPLERAGDAIASLENRTAKGKVVVKLR"
     gene            2667..>3055
                     /locus_tag="VGP27_20960"
     CDS             2667..>3055
                     /locus_tag="VGP27_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7853803.1"
                     /translation="MGPSEVLRRLATPRRRSTVPVGGFDAATGQWTDSVTADGVERDE
                     VTLTTFNIWNESKHAERRYQAIADLLSARGPDIMVFQEITRSALRVFLAQRWIRDSYV
                     RAAVSGFRVGNYGMLMLSRLPVTDVTYT"
BASE COUNT          492 a         1020 c         1089 g          454 t
ORIGIN      
        1 gcgcagcact ggccgcaggg aaacctgagc aaggcctacg ccgcctaccg gacaccgttc
       61 tggcgcgcga acggatgcag cggcgaggcg ctgtcggacg agggcccggt gttcatcacc
      121 ttcgacgtca gccccggcgg cgacgacaac cccgggatcc tgttgggctt caccgacccg
      181 cgcacgttcg atccgctgcc gcccgccgag cgccgcgagc gcgcgctggc agggttcgcg
      241 gcgctgttcg gcgacaaggc tctggaaccg atcgactacg tcgaccactg ttgggccagt
      301 gaggaattcg cgcccggcgg accgaccgcc gcggtaccgc ccgggtcatg gaccacctac
      361 ggcccgtggc tacgcaagcc ggtgcaggga atcttctggg cgggcaccga aaccgctgac
      421 gagtggaccg gcttcctcga cggcgccgtg cggtcgggtc gtcgcgcagc cgccgaggtg
      481 cacggcgaac tgtcgggcta agacgtggcg cgccgcatct cggcgaccga ctcgatgagc
      541 cagcgcaagt ggccgttgac ctcgtcgggg tgctcgagga tcgcgcagtg cccgccggaa
      601 agctcgacga tatccgcgag gttgggcgcc gtccgcgcga tgtgacgcga cgacacgatc
      661 ggcagaaggc gatcctgccg gctgccgatc accagcgtcg gcacggtgag gttctgcaga
      721 ccgatgtgct ggtgggtgcc cagggagtcg atcagcttgc gggcccagcc gccgcgactc
      781 gcggcgggcg tctcggtgaa cagttggtag acgaactccc cgaccgccgg gtccgcgtcc
      841 cgaccaacgg ccatcatcgt cacgaaccgc ttgctgggcc ggtccgcggc acgcagcagg
      901 ggcgccgcac cgaagctcct gagaaggctt tccgcggcgc gaacgcgggc gttggtgagc
      961 ggccggggaa cggccgccag cttcacatgg cgcagcaggt ccccggtggt ggtgttgatc
     1021 agggctacgg cgtcggcgcg ttcggtcacc cggcgcggat accgctcgga ccacgacgcg
     1081 atcgcgatgc cgcccatgga gtggccggcc agcacggccc gttcgccggg cttcagcgtc
     1141 gcctccagaa cggcgtcgag gtcggcggca aggaagttca gcccgtagtt cgcgcgccgc
     1201 gacggcgcgc cgctgcgacc atggccgcgg tggtcgtaag cgatgacgcg gtactcgcag
     1261 gccaggtcgg cgatctgata cgcccacacc cggagggcgc aggtgatgcc gtgcgccagc
     1321 acgatcgggt agccgtcctc gggtccgaag acctcggcgt gcagcgcagt gccgtcgccg
     1381 gcgcgcacgt tcagcggacg tgacggcggc agagcgtcgg ccgcagctaa ccgtcgcgca
     1441 gtgcggttga cccgctgacc tctcggcata gctggcagat attaccgtca ggcggcagcc
     1501 gtaccgtcga cccgaggctg ttaaatgggg cgatgcgcgc aattcagata ccccgcctcg
     1561 acggtcccgc ggcggcggag ctcgtcgagc tcgaagagcc caccgacgag aacgcggtca
     1621 tcgtcgaggt gcacgcggca ggggtggcct ttcccgacgc cctgcagtcg cgcgggctct
     1681 atcagtacaa ggcggagttg ccgttcgtgc ctggcgccga aatcgcgggc gtggtgcgca
     1741 gcgcaccctc cggcagcggc ttcgccgcgg gcgatcgggt cgcaggcctg acgatgctga
     1801 acggcgggat ggccgaagtg gttgcgctgc aaccggagag agtgttcaag ctgcccgaca
     1861 acgtgtcctt cgaagccggt gcggggctgc tgttcaacga cctcacgatg catcacgcgc
     1921 tgcgcacccg cggcaggctg accgacggtg agaccgttct ggtgcacggc gctgccggcg
     1981 gaatcggcac ctcgacactg cgtttggcgc cggtgtgggg cgcaacgcgg accatcgcgg
     2041 tggtcggcac cgaagacaag aaagcgatcg ccaagtccgc gggcgcatcc gacgtggtgc
     2101 tggccgacgg cttcaaggac gccgtcatgg ggctgacgga cggcaagggc gtggacatcg
     2161 tcgtcgaccc ggtcggcggt gaccggttca ccgactcact gcgttgcctg gcacccggcg
     2221 gtcggctgct gatcatcggg ttcaccggcg gagagatccc caccatcaag gcgaaccggc
     2281 tattgctcaa caacgttgac gcggtcggcg tcggctgggg cgcgtggacg ttcacccatc
     2341 ccaactacct gcgcgaacag tgggacgact tggagccgct gctggcgtcg ggcgcggtgt
     2401 ccgccccgga accggagatc tatccgctgg agcgcgcggg cgacgccatc gcatcgctcg
     2461 agaaccgcac cgccaaaggc aaagtcgtcg tcaagctgcg gtgaggccgg ctttctggcc
     2521 gttggcagag gcggccggtt accacagcgc ccgcggtgag atcgttgcga cgggacagag
     2581 cactgcgaac ggcccggatg caggacgacg cgccactagc ggggcaccca ccctcggtca
     2641 cgggcccatg ctggaaactc ttgggcatgg gcccttccga ggtgctgagg cggctggcga
     2701 cgcctcgtcg ccgcagcacg gtgcccgtcg gaggcttcga cgcggccaca ggtcagtgga
     2761 cagacagcgt caccgcagac ggtgtcgagc gtgacgaggt cacgctgaca accttcaaca
     2821 tctggaacga aagcaaacac gccgaacggc gctatcaggc gatcgcggac ctgctgtccg
     2881 ctcgcgggcc cgacatcatg gtgttccagg agatcacgcg ctcggctctg cgggtgttcc
     2941 tcgcgcagcg atggattcgc gacagctatg tccgcgcggc cgtcagcggc ttccgggtcg
     3001 gcaactacgg catgttgatg ctttcgcgtc ttcccgtcac cgacgtgacc tacac
//
LOCUS       DASYLX010000898         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2139489,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000898 DASYLX010000000
VERSION     DASYLX010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139489"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..940)
                     /locus_tag="VGP27_20965"
     CDS             complement(8..940)
                     /locus_tag="VGP27_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853804.1"
                     /translation="MNDVVDCAVVGAGAAGLSAALVLGRARRRTLVIDAGEQSNRASD
                     VIGGLLGYDQRPPAELYAAGGEELASYPSVECRGGRVLRGRRVEGGFLLQVDDGSEIC
                     ARRVLLATGMDYCPPQLPGLAQLWGTSVFQCPFCHGWEMRDKRLAALAAGGAAIHAGL
                     MLRGWTDDLVVLTDGPVEMSDESLRILRSADVTVDDRRIVELIGEQGQLTEIAFADGT
                     RLPRDGLLVEAPLRQRSPLAQQLGVADTAGSVGVDEMKVDALHCTTVDGVFAAGDNCT
                     KHPSLADAINAGSKAAMMIVQSLLADEYGLPYPP"
     gene            1125..1448
                     /locus_tag="VGP27_20970"
     CDS             1125..1448
                     /locus_tag="VGP27_20970"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEV7853805.1"
                     /translation="MVASTSRDELRKCLEDIDFPAGKDDLVNAASKKNCDEAARALRA
                     IPPETYSNLAEVLASVRLDDDELSEAEKASARRTHTKPGLAENAKEIGPVNPIVEELG
                     ENRYS"
     gene            1718..2419
                     /locus_tag="VGP27_20975"
     CDS             1718..2419
                     /locus_tag="VGP27_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7853806.1"
                     /translation="MAARASLEHMLGEDSDSLAGQLALVDFPSGHDVFVEGDTAETMY
                     IVVRGKVKIGCRTAEGRQKLTAVIGGGDIFGAVSVLDSGPRSANATAITDLCVATLHQ
                     HALTAWTREHPQCSERLLRVLARRLQRAEDELAELTVVDVAGRVAHRLLKLAQRFGHS
                     DGDGIVVTHDLTQDEIAQLVGATRESVNKALCEFARLGWITTNGRTMFIHRTQPLVQR
                     ARQTIDRHNSSDSER"
     gene            complement(2408..>3208)
                     /gene="nrfD"
                     /locus_tag="VGP27_20980"
     CDS             complement(2408..>3208)
                     /gene="nrfD"
                     /locus_tag="VGP27_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEV7853807.1"
                     /translation="GRPGLRKVTRIGSLVSIVASMYFLVADLGRPERFHHMLRVAKPS
                     SPMSMGTWILSAYGPGAAVAAIAELMPKRLRGTWLGRLVNWVARPAGLWAAGTAPGVA
                     SYTAVLLSQTAVPAWREAHPYLPFVFTGSAAASGAGLGMLFAPVDEIGPARRLAVTGA
                     VLEVAASRTLEHRLGLSAEAYTTGKPHRLRKWAEILTVGGAVGAAVGGRNRAVQAASG
                     AALLTGSALQRFGVFEAGVASTRDPRYVVVPQRERLDAGLPVRAQSAL"
BASE COUNT          589 a         1151 c         1016 g          452 t
ORIGIN      
        1 gaaaacgtca cggcggatag ggcagtccgt actcgtcagc cagcaaactc tgcacgatca
       61 tcatcgccgc cttcgaaccg gcattgatcg cgtccgcaag ggaagggtgc ttggtgcagt
      121 tgtcgccggc ggcgaagaca ccgtcgaccg tcgtgcaatg aagggcgtcc accttcattt
      181 cgtccactcc caccgatcca gcggtgtcgg cgacacccaa ctgttgcgcc aacggcgacc
      241 gctgccgcag cggcgcctcg accagtaagc cgtcccgcgg caaccgcgtc ccgtcagcga
      301 acgcgatctc ggtcagttga ccctgctcgc cgatcaattc gacgattcga cggtcatcca
      361 ccgtcacgtc agcggacctc aatatccgca ggctctcgtc gctcatctcc acgggcccgt
      421 cggtaagcac cacgaggtcg tcggtccacc cccgcagcat cagccccgcg tgaatggccg
      481 cgccaccggc ggccagcgcc gccagccgct tgtcccgcat ctcccagccg tgacagaacg
      541 gacactggaa caccgacgtg ccccacaatt gcgcgagacc gggcaactgc ggcgggcagt
      601 agtccatccc ggtcgccagc agcacccgcc gggcacagat ttccgagccg tcgtcgacct
      661 gaagcaggaa accgccttcg acacggcgac cgcgcagaac ccgcccaccg cgacattcga
      721 cgctcggata tgacgccagc tcctctccgc cggccgcata caactcggcc ggcggccgtt
      781 ggtcgtatcc gagcaggccc ccgatgacgt ccgacgcccg gttgctctgc tcgccggcgt
      841 cgatcaccag cgtccgccgc cgcgcccggc ccagcaccag ggcagcactc aaaccggccg
      901 ctcccgcgcc gacgacggcg cagtccacca catcgttcat ccggtcggtc gaagtcgtcc
      961 ttgcgccatg aggcgtgggt accccgcgtt ctggctaatc acacccgcga atcacgcatc
     1021 gacgtggcaa ctacgccggc ggttcggtgg tggttcgagt gccgcgtgaa gggtagcccc
     1081 acccatgaac tcagcttcca actgagaaga ctaggagaaa cgtcatggtc gcatccacat
     1141 cccgcgacga gctacgaaag tgcctcgagg acatcgactt tccagccggc aaggatgatc
     1201 tggtgaacgc cgcctcgaag aagaactgcg acgaggccgc ccgcgcgctg cgggcaatac
     1261 cgccggagac ctacagtaac ctcgccgagg tgctcgcctc cgtcaggctc gacgacgacg
     1321 aactcagcga ggccgaaaag gcctcggcgc gtcgcacgca caccaagccg ggcctcgccg
     1381 agaacgcaaa agagatcggg cccgtcaacc ccatcgtcga ggaactcggc gagaaccgct
     1441 actcgtagac tccagccggc ggcacggttc tgcggcccgg ccgcggtcaa accgatggag
     1501 cgcgcgcaaa cgtccgaagg acgaaaccgc gcgggccagt tggcgaaaca ctgtgtcgcg
     1561 gccggttgat cgacgtcgtt ggcgggtaaa tcgctgcgca tgtcgcaggc aggtcacatt
     1621 tcaccggtgc cacatggcgc gtatcgctga acccggacag cgccgggcaa ccggcgctcc
     1681 ggccgccgac gccagaagtc atcgacgcgc cgacatcatg gccgctcgtg cgtcactgga
     1741 gcacatgctc ggcgaggatt ccgacagcct tgccggtcaa cttgcattgg tcgatttccc
     1801 gtccgggcac gacgtcttcg tcgaagggga caccgcggag acgatgtaca tcgtcgtgcg
     1861 ggggaaggtg aagatcggct gccggacggc tgagggcagg cagaagctga cggctgtcat
     1921 agggggcggg gacatcttcg gtgcggtatc ggttctcgat tcggggccgc gctcggcgaa
     1981 cgcgacggcg atcacggatc tctgcgtggc gacgctgcac caacacgcct tgacggcctg
     2041 gactcgagag catccgcaat gcagcgagcg gttactgcgg gtactggcac gccgacttca
     2101 gcgcgccgaa gacgagctcg cggagctgac ggtcgtcgat gtcgccgggc gggtggcgca
     2161 ccgattgctg aaactcgcgc agcgcttcgg ccacagcgac ggtgacggca tagtcgtgac
     2221 tcatgacttg actcaggatg agatcgcgca gctggtcggg gccaccagag aatcggtgaa
     2281 caaggcgctg tgcgaattcg cgcgactcgg ctggatcacg acgaacggcc gaaccatgtt
     2341 catccataga actcaaccgc tggtgcagcg tgcccggcag acgatcgacc ggcacaactc
     2401 ttcggactca gagcgctgat tgcgcccgaa cgggcaggcc cgcgtcgagc cgctcgcgct
     2461 ggggcaccac gacgtagcgc gggtcgcgcg tcgacgccac gccggcctcg aagacgccga
     2521 agcgttgcag cgcgctgccc gtcagcagcg ccgcacccga cgcggcctgc accgcgcggt
     2581 tgcggccgcc caccgccgca ccgacggcac cgccgaccgt caggatctcg gcccacttcc
     2641 gcagccggtg cggcttgccg gtggtgtagg cctcggcgct gagcccgagg cgatgctcca
     2701 gcgtgcgcga tgcggcgacc tccagcacgg cgccggtgac ggcgagccgt cgcgccgggc
     2761 cgatctcgtc gacgggcgcg aacagcatgc ccaacccggc gccgctggcc gccgccgagc
     2821 cggtgaagac gaacggaaga tacgggtgcg cctcccgcca cgccggaacc gccgtctgcg
     2881 acaacagaac cgcggtgtac gacgcgacac ccggcgcggt gcccgccgcc cacagtcccg
     2941 ccggcctcgc cacccagttc accagccggc caagccatgt ccctcgcagg cgcttgggca
     3001 tcaactccgc gatcgcggca acggcggcgc caggcccata tgccgacagg atccacgtgc
     3061 ccatgctcat cggcgagctc ggcttggcca cccgcagcat gtggtggaat cgctccggcc
     3121 ggccgaggtc cgcgaccagg aagtacatac tcgccacgat gcttaccagc gaaccgatcc
     3181 gggtcacctt gcgcaacccc ggccgccc
//
LOCUS       DASYLX010000899        10090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3455673,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000899 DASYLX010000000
VERSION     DASYLX010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455673"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..630)
                     /locus_tag="VGP27_20985"
     CDS             complement(160..630)
                     /locus_tag="VGP27_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853808.1"
                     /translation="MIFSAVAASMVVSTPQAQADPGDGIALNGTYTAFSDGVWAKTND
                     SYHNERSVTQTWTISSTCSTYQDCTGRVTSDQGWSTDMIYMSGSWRVRRTFDNWEPCI
                     DGTATPGEQTFTFWKGRPDAFPMKGWDTTKGPSGACGRNKQLGVQLPFTLTPVT"
     gene            complement(642..2264)
                     /locus_tag="VGP27_20990"
     CDS             complement(642..2264)
                     /locus_tag="VGP27_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7853809.1"
                     /translation="MLTRFVRIQLAAFTIASIVGVLVMVFVYMQAPTLLGIGRITVKL
                     ELPASGGLYRFSNVTYRGVQVGKVTGMDVSRTQAIATLSLGSSPKIPADLDAEVRSVS
                     AVGEQYVELMPRTDSPPYLQDGSVIAMADTKTPQTVGPMLDQVNTLINSVPKKTLTGL
                     IDESFNAFNGAGYDLGSLFDSSAKISGDLNKVPDQSSALIEDTGPLLDSQAQTTDSLR
                     LWARSLAGITGQVVTNDPQVRTLLQEGPPAANEVSRLLDQVKPTLPVLLANLTTIGQV
                     AVTYHPSLEQVLVLLPPFVGDVLSSAPENNPTGIPLGDFRIQIGDGPPCTVGFLPPSQ
                     WRSPADLTEMDTPDGLYCKLPQDAPVVVRGARNSPCMGVPGKRAPTVQECYSDKPYTP
                     LAVRQHALGPYPFDPNLIAQGIPLDDRVDANERLYAPVEGTPVPPGAVPSGTPPDAPP
                     LGAPPPPPPADAPPPAPPAAVFPPMDSGGAVPAAPSSFSVNGSGPGPSVAVAHYDPQT
                     GQYAAPGGQVFRQEDLVDPAKSWQDMIYRADA"
     gene            complement(2264..3382)
                     /locus_tag="VGP27_20995"
     CDS             complement(2264..3382)
                     /locus_tag="VGP27_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853810.1"
                     /translation="MTVTGCAFQGVNSLPLPGAVGRGGDAAVYHVELANVGTLESNSP
                     VLIEDVVVGSVGTMRLHGWHIDLDLALKPDAIVPANAVATVGQTSLLGSMHVALDPPP
                     GEAPSGRLKPGATIGLNSSSTYPSTEHTLSSLAAVVNGGGLGQLGDIVHNFNIALNGR
                     QDVVRDLIARLDTFVGTLYRQRDNIIATIDELNRFSSTLGGQQEVLTRALNKIPPALD
                     VLMRERPRLTTALEKLGTFSDTVSGLVNDTQADLVTNLQNLEPTLKALADVGSQIDTA
                     LAYIPTFPLTQNLIDRGVRGDYVNLFVTIDLTNSRIKRGLLLGTRLGQDRASMIPAPG
                     DPGYDAYYTKFPLGVGTSPPFGPIPNADPAPWEPGGGG"
     gene            complement(3433..4848)
                     /locus_tag="VGP27_21000"
     CDS             complement(3433..4848)
                     /locus_tag="VGP27_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853811.1"
                     /translation="MTRNRLLKLALAPVLAVALVGGVAVVVQQTFFRPNTITAYFTKT
                     VAIYPGDEVRIAGVKVGTIDSIEPVGTQAKMTLRVNRRISVPIDAKAVIVAQNLISAR
                     YVQLAPAYESGPKMTDGAVIPLDRTAIPVEWDEVKEQLMRLATELGPDKGMSTGSVGR
                     FIDSAANALDGNGDKLRQTLAQLSGVGRILADGSGNIVDTISNLQTFVTALRDSNNQI
                     VQFQDRFATLTSVVDDSRSDLDAALKNLSEVVGETQRFIEGTRDKTAEQIRLLASVTQ
                     NLADHRMDLENVLHIAPHSIANAVNMFDPRTGAASGVFVLNNLSNPVWAVCTMMGALE
                     NVTSPETGKLCSQYVGPALRTLNFNNLPIPINPFLTSNPPDYMLRYSEDRLRPENEGQ
                     PPPPYTDPPAVSAYTGAGDISPPPGYGPPPGPPPAASLPMAPPPAPPAPTLQDMLLPA
                     EAPPPPGDAPTGPPPGDGTPR"
     gene            complement(4845..5948)
                     /locus_tag="VGP27_21005"
     CDS             complement(4845..5948)
                     /locus_tag="VGP27_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853812.1"
                     /translation="MLKYRGSKLVRTGFMGVVLAVLIVLVGLSPETLTSMATAIRYQA
                     QFSDAGGLTEGNDVTVAGIKVGSVSGVSLEGSNAVVTFAVDGTVELGSETTAHVRTGT
                     LLGERVLTLESAGEDSMHPMDLIPVSRTASPYSLTEAVSELTTNTAGTDTASLNQSLN
                     TLSETLNQVAPQLGPAFDGITKLSQAINDRDDTLGALLSDGADVTKILSQRSAQVNAL
                     ILNGNDLVGVLNERRQAIVEVLVHTSALAKQLSGLIHDNEEKLAPTLDKLNAVTAVLE
                     KNRDNIAKAIPGLAKFQTTLGETIGNGPYYQAYVPNLLISELFQPFFDYAFGFRRGTN
                     AGQPPDNAGPRAELPFPYNGIPQPGEQWGPPPP"
     gene            complement(5948..6973)
                     /locus_tag="VGP27_21010"
     CDS             complement(5948..6973)
                     /locus_tag="VGP27_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853813.1"
                     /translation="MRRSLRPTLIKFGIFGIVMAVLTACLFFIFGQYQTGSTTDYSAV
                     FKDASRLKVGQSVRVGGMRVGTVNSVKLQKDKTALVIFDADPAVQMTTGTQVAVRYLN
                     LVGDRYLELIDGPGDRRLPPGSQIPRERTQSALDIDLLLGGLKPVIRGLNPQDVNALS
                     ASLIQIFQGQGGTLQSLLSNTSGFTTALAEHNQTVQALIDNLRTLLSTLTKEGEKLSG
                     TIAKLETLVTQLAAEKDPIGTAIDSLNNGTASVADLLTDARPPLKGTVDQLNRLVTNM
                     DGQKDRLDTSLQKAPENYRKLIRTGSYGSFFNYYICSLTWRVTDLQGRTAEFPWIRQD
                     GGRCTEP"
     gene            complement(6970..8130)
                     /locus_tag="VGP27_21015"
     CDS             complement(6970..8130)
                     /locus_tag="VGP27_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7853814.1"
                     /translation="MGVATVVAIIAVFALSATLFRGGLTDSVPVTVMSPRAGLVMNPD
                     AKVQIRGVQVGKVASIEQLPNGEAALHLAMDPKRMSAIPANVLVDIASTTVFGAKYVQ
                     LVFPPDPSPESLRAGQVLAAKHVMVEINTVFENLTSVLSSIDPPKLNETLGAIASSVN
                     GRGKKIGQMLTDLDAYLAKIEPSLPALSHDLETAPQVLQAYGDAAPDFVTVADNAARL
                     SDTLVDQQQNLDTALVSVIGLADIGNDVIGTNRQPLTDVLHLLVPTTDLLNKYNEALW
                     CALSGMANASARPPLKEPGVQVLAGFLWGQERYRYPMDLPKAAATGGPQCTGLPNLPF
                     EGAPPYVVADTGTNPWRRTYPGIVLNSDGLKKILFGEIDGPPRNTYQIGQPG"
     gene            complement(8891..10072)
                     /locus_tag="VGP27_21020"
     CDS             complement(8891..10072)
                     /locus_tag="VGP27_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853815.1"
                     /translation="MYYDPFDFAIDDDPYSVWKRMRAEAPLYFNEKYNFYALSRYDDV
                     ARALPDWQTYRSGRGTTADILFSGIEVPPGILLFEDPPLHDLHRRLLSRVFTPRRMLA
                     VEELVREFCSRALDPLRGADGFDFVVDLGAIMPMRTIGYLLGIPEEGQQQIRDRNDKS
                     ITVSSRHGEDGDLSQTIIGESIAMFAEYIQWRATHPSDDLMTELLNAEIEEQDGTRRR
                     LERTELLAYTAMIAGAGNETTARLIGFMGQLLGDHPDQRRELVADSSLISSAVEETLR
                     YEPPSPVQARYVASDVEHYGRTVAEGSYMLLLNGSANRDETRFAEPDRYDIHRKGGHL
                     SFGQGLHFCLGSALARLEGRVALEEVLKRWTDWDVDYGNASRARTSSVRGWARLPVTT
                     G"
BASE COUNT         1694 a         3130 c         3335 g         1931 t
ORIGIN      
        1 accggtcgcg gaagactgtc aacgtaatcg ccgatttctg cgcgacggct gttgtcggca
       61 caaaatcgcg accctcatgc agaaatggcg gataagtcga aggcgtgccg agcgatttcg
      121 gcgtgctgcg ttgcgctgcg ccaaccagcc acgccgaaat cacgtgacgg gggtgagagt
      181 gaagggcagc tggacaccga gctgcttgtt gcgtccgcat gcaccgctcg gacccttcgt
      241 cgtgtcccag cccttcatgg gaaacgcgtc cgggcggccc ttccagaacg tgaaggtctg
      301 ctcccccggc gtcgcggtgc cgtcgatgca cggttcccag ttgtcgaagg tgcggcgaac
      361 tcgccaactg ccgctcatgt agatcatgtc cgtgctccag ccctggtcac tggtgacacg
      421 tccggtgcag tcctgatagg tgctgcacgt cgagctgatc gtccaggtct gggtcacgct
      481 gcgctcgttg tgataggagt cgttcgtctt cgcccacaca ccgtccgaga acgccgtgta
      541 ggtgccgttc aatgcgatgc cgtcgccggg atctgcctgc gcctgtggcg tgctgaccac
      601 catcgacgcc gccactgcac tgaagatcac gcgcgcgagc ttcatgcgtc cgcccggtag
      661 atcatgtcct gccacgactt ggcgggatca acgagatctt cctgccggaa gacctgacca
      721 cccggtgcgg cgtattgccc ggtctgcgga tcatagtgcg cgaccgcgac cgacggtccc
      781 ggcccggatc cgttgacgct gaacgaactc ggcgccgcgg gcacggcacc tccggagtcc
      841 atcggcggga agaccgccgc cggcggagct ggcggagggg cgtcggccgg cggtggtggc
      901 ggcggcgcgc ctagcggtgg cgcatcgggc ggggtgcccg acggcactgc ccctggcggc
      961 accggggtgc cctccacagg tgcgtagagc cgttcgttgg catcgacgcg atcgtccagc
     1021 gggatacctt gcgcaatcag attgggatcg aagggatacg ggcccagcgc atgctgccgg
     1081 accgccagcg gcgtatacgg cttgtcgctg tagcactcct gcaccgtcgg cgcgcgcttg
     1141 cctggcacac ccatgcacgg tgagttgcgc gctccgcgca caacgaccgg cgcgtcctgt
     1201 ggcagcttgc aatacaagcc gtccggagtg tccatctccg tcagatcggc gggggaccgc
     1261 cactgcgacg gtggcaggaa cccgacggtg cacggcggcc catcaccgat ctgaatcctg
     1321 aagtcgccca aggggattcc ggtcgggttg ttctcgggag ccgaggacag cacatctcca
     1381 acaaacggcg gaagcaacac cagaacctgc tccagtgacg ggtggtaggt gaccgcgacc
     1441 tggccgatcg tggtgaggtt cgccagcagt accggcagtg tcggcttcac ctgatcgagc
     1501 agtcgagaca cctcgttcgc cgcgggcggt ccttcctgca gcagtgtgcg cacctggggg
     1561 tcgttcgtca ccacctgccc cgtgatcccg gccaggcttc ttgcccatag tcgcagcgaa
     1621 tccgttgtct gtgcttgcga atcgagcagc ggcccagtgt cttcgatgag cgccgacgac
     1681 tgatccggca ccttattgag atcaccggag atcttcgccg aggagtcgaa cagcgagccg
     1741 aggtcatagc cggccccgtt gaaggcattg aacgactcgt cgatcaaccc ggtgagcgtc
     1801 ttcttcggca cgctgttgat cagggtgttg acttgatcga gcatcgggcc gaccgtctgc
     1861 ggggtcttgg tgtcggccat cgcgatcacc gagccgtcct gcaggtacgg cggcgaatcc
     1921 gtgcgcggca tcagttcgac gtactgttcg ccgaccgccg agacgctgcg cacctctgcg
     1981 tccagatcgg ccggaatctt cggtgagctc cccaatgaca gcgttgcgat cgcctgtgtg
     2041 cgcgacacgt ccatccccgt caccttgccg acctgaacgc cgcggtacgt cacgttggag
     2101 aaccggtaca gcccgcccga cgcgggaagt tccagtttga ccgtgattcg tccgatgccg
     2161 agcagggtcg gcgcctgcat gtagacgaag accatcacca gcacgccgac gatcgacgcg
     2221 atcgtgaagg cggccagctg gatgcggacg aacctggtca gcatcagcca ccaccgcccg
     2281 gttcccacgg tgccgggtcg gcattcggga tcggcccgaa cggtggcgac gtgccgacgc
     2341 cgagcggaaa cttggtgtag taggcgtcat atccgggatc gccgggcgcc gggatcattg
     2401 acgcccgatc ctggccgaga cgggtaccga gcagaaggcc ccgcttgatt cgggagttcg
     2461 tgagatcgat tgtcacgaag aggtttacgt agtcgccgcg gacgccccgg tcgatgagat
     2521 tctgggtcag cgggaacgtc gggatgtatg ccagggcggt gtcgatctgc gaacccacgt
     2581 cagccagcgc cttgagcgtg ggttcgagat tctgcaggtt ggtgacgagg tcagcctgcg
     2641 tgtcgttgac gagcccggag acggtgtcgc tgaaagtgcc gagtttctcc agcgcggtgg
     2701 tgagcctcgg tcgctccctc atcagcacat ccagcgcggg cgggatcttg ttcaatgccc
     2761 gcgtgagcac ttcctgttgg ccgccgagcg tcgaggagaa ccggttcagt tcgtcgatgg
     2821 tggctatgat gttgtcgcgc tgtcgataaa gggttccgac gaacgtgtcg aggcgagcga
     2881 tcaaatcgcg cacgacgtct tggcgcccgt tcaacgcgat gttgaagttg tggacgatgt
     2941 cgccgagctg ccccagtccg ccgccgttga ccacggcagc aagcgacgac aacgtgtgtt
     3001 cggtcgacgg gtaggtggac gaactgttca gaccgatcgt ggcgcccggc ttcaggcgac
     3061 cgctcggcgc ttcgcccggt ggcggatcga gcgccacgtg catcgaaccc agcaggctcg
     3121 tctgcccgac cgtcgccacg gcgttggcgg gcacgatggc gtccggcttg agcgctagat
     3181 ccaggtcgat atgccagccg tgcaaccgca ttgtgccgac gctgccgacg acgacatcct
     3241 cgatcagcac cggtgaattc gattccagcg tcccgacatt cgccagctcg acatggtaaa
     3301 cggcggcatc gccaccacgc ccgaccgcgc caggcagcgg tagcgagttc acgccctgga
     3361 atgcacaccc ggtgacggtc accacaatgc tgcagccgat agccagaagt cgacgcccgc
     3421 actccgcccc gatcaccgcg gtgtcccgtc acccggcggc ggccctgtcg gtgcgtcgcc
     3481 cggcggtggc ggcgcctctg ccggcagaag catgtcctgc agcgtcggcg ccggtggcgc
     3541 aggcggtggc gccatgggca gcgacgccgc gggcggtggg cccggcggcg gcccgtagcc
     3601 cggcggcggg gagatgtcgc cggcacccgt gtacgcggaa accgctggcg gatcggtgta
     3661 cggcgggggt ggctgtccct cgttctcggg cctgagcctg tcctcggaat atctgagcat
     3721 gtagtccggg ggattggacg tcaggaacgg gttgatcgga atcggcaggt tattgaagtt
     3781 gagggttcgc agcgcggggc cgacgtactg ggagcagagc ttgccggtct caggagacgt
     3841 cacgttttcg agcgcgccca tcatggtgca gaccgcccag accgggttcg acaggttgtt
     3901 gagcacgaac acaccgctgg cggccccagt ccgggggtcg aacatgttga cggcgttggc
     3961 gatcgagtgg ggtgcgatgt gcagaacgtt ctcgaggtcc atccggtgat cggccaggtt
     4021 ctgggtgaca ctggcgagca accgaatctg ttcggcggtt ttgtccctgg tgccttcgat
     4081 gaaacgctgc gtttcgccga cgacttcgga caagttcttc aatgcggcat cgaggtccga
     4141 tctgctgtcg tcgacgacgc ttgtcaacgt cgcgaaccgg tcctggaact gcactatctg
     4201 gttgttgctg tctcggaggg cggtcacgaa cgtctgcagg ttgctgatgg tgtcgacgat
     4261 gttcccgctg ccgtcggcaa gaattcttcc gaccccggac agctgggcca gcgtctgccg
     4321 caacttgtct ccgttgccgt ccagagcgtt ggcggcactg tcgatgaatc gcccgaccga
     4381 gcccgtcgac atacctttgt ccggccccag ttccgttgcc agccgcatca actgctcttt
     4441 gacttcgtcc cattcgacgg ggatcgccgt gcggtcgagt ggtatcaccg cgccgtcagt
     4501 catctttggg ccggattcgt aggcgggcgc gagctggacg tagcgggccg agatcaggtt
     4561 ctgggcgacg atcaccgcct tggcatcgat gggaaccgag attcggcggt tgacccgcag
     4621 tgtcatcttg gcctgtgtgc cgaccggttc gatcgagtcg atggtgccca ccttgacacc
     4681 ggcgatgcgg acctcgtcgc cggggtagat ggcgaccgtc ttggtgaagt acgcggtgat
     4741 cgtgttgggc cggaagaagg tctgctgcac gaccacggcg acgccaccca cgagcgccac
     4801 cgcaagcacc ggcgcgagag ccagtttcag gagccggttc cgagtcatgg cggtggcggc
     4861 ccccactgct cgccgggctg cggaataccg ttgtacggga acggaagctc agcgcgtgga
     4921 ccggcgttgt caggtggctg tccggcgttg gttccccgcc ggaagccgaa cgcgtagtcg
     4981 aagaacggct ggaagagctc gctgatcagg agattgggaa cgtaagcctg gtagtagggg
     5041 ccgttgccga tggtctcgcc gagtgtggtc tggaatttcg cgagccccgg tatcgccttg
     5101 gcgatgttgt cccggttctt ctccagcacc gcagtcaccg cattgagctt gtccagggtc
     5161 ggggccagct tctcctcgtt gtcgtggatg aggcccgaaa gctgcttcgc caacgccgat
     5221 gtatgaacca gcacttcgac gatcgcttgg cggcgctcgt tgagtacacc gacgagatcg
     5281 ttgccgttga gaataagcgc attcacctgt gcgctgcgct gcgacagaat cttcgtgacg
     5341 tcggcaccgt cggagagcag tgcgcctagc gtgtcgtctc ggtcgttgat ggcctgggag
     5401 agcttcgtga tgccgtcgaa cgccggaccg agttgtggcg caacctggtt caaggtctcc
     5461 gacagtgtgt tcaacgactg gttgagagaa gcggtgtcgg tgcccgcggt gttggtggtc
     5521 agctcgctga ccgcttcggt cagcgagtac ggcgaagctg tacgcgatac cgggatgagg
     5581 tccatgggat gcatgctgtc ctcgccggcc gattccaagg tcaggacgcg ttcaccgagg
     5641 agcgtcccgg tccgcacgtg tgccgtcgtc tccgagccga gttctacggt gccgtcgacg
     5701 gcgaatgtca ctaccgcgtt gctgccctcc agcgagacgc cggagacact gcctaccttg
     5761 atcccggcga ccgtgacgtc gttaccctcc gtcaggccac cggcatcgga gaactgcgcc
     5821 tgatagcgga ttgccgttgc catcgacgtc aaggtctcgg gggagagtcc gacgaggacg
     5881 atgagcaccg ccagcacgac tcccatgaat cccgttcgaa caagtttact tccacggtat
     5941 ttcaacatca gggctcggtg cacctccctc cgtcttgcct gatccagggg aactcggcgg
     6001 tgcggccctg aaggtcggtc actcgccagg tgagtgagca gatgtagtag ttgaagaagc
     6061 tgccgtacga gccggtgcgt atcagcttgc ggtagttctc gggcgccttc tgcagtgaag
     6121 tgtcgagccg gtccttctgg ccatccatgt tcgtgaccaa ccggttcaac tggtcgacgg
     6181 tgcccttgag tggaggccgg gcatcggtga gtaggtcagc cacggatgcg gtgccgttgt
     6241 tcagcgaatc gattgcagtg ccgatcggat ccttctccgc cgccagctga gtcaccaacg
     6301 tctccaactt cgcgattgtg ccggataact tttcgccttc cttggtcaag gtcgagagca
     6361 gcgtgcgcag attgtcgatc agcgcctgga ccgtctggtt gtgttcggcc agcgcggtgg
     6421 tgaaccccga tgtgttggac agtagcgact gcagggttcc gccctgcccc tggaagatct
     6481 ggatcagcga cgccgacaag gcgttcacat cttgcggatt gagccctcgg ataacgggtt
     6541 tgaggccgcc gagaagcaga tcgatgtcga gggccgactg ggttcgctcc ctgggaatct
     6601 gcgatccggg cggcaaccgc cggtccccgg gcccatcgat cagctccaga tagcgatcgc
     6661 ctacgaggtt gaggtaacgc acggccacct gggtgccggt ggtcatctgg acggcgggat
     6721 cggcgtcgaa tatgacgagc gccgtcttgt ctttctgcag cttgacgctg ttgaccgtgc
     6781 caactcgcat acccccgaca cggaccgact gtccgacctt gaggcgcgag gcatccttga
     6841 acaccgctga gtagtccgtc gtcgagccgg tttggtactg cccgaagatg aagaacagac
     6901 atgcggtcaa gaccgccatg acgattccga agatgccgaa tttgatcagc gtcggacgta
     6961 gcgagcgcct catccgggct ggccgatctg ataggtgttg cggggcggcc cgtcgatctc
     7021 accgaacagg atcttcttca ggccgtcgga gttcagcacg attccgggat aggtgcgccg
     7081 ccacgggttg gtccccgtgt cggcgacgac gtagggaggc gccccctcga acggcaggtt
     7141 gggcaggccg gtgcactgcg gaccgcccgt cgcggccgcc ttcggcaagt ccatcgggta
     7201 gcggtaacgc tcctgccccc acaggaagcc ggcgagcacc tggacccccg gttccttcag
     7261 cggcgggcga gccgaagcgt tcgccatccc gctgagggcg caccacagcg cctcgttgta
     7321 cttgttcaat agatcggtgg tcggcaccag taggtgcaga acatcggtga gcggctgccg
     7381 gttggtgccg atcacgtcgt tgcctatgtc ggccagcccg atgacgctga cgagtgcggt
     7441 gtcgagattc tgctgctggt cgacgagtgt gtcgctgagg cgcgcggcgt tgtcggccac
     7501 cgtcacgaag tcgggcgccg cgtcaccgta ggcctgcaga acctgaggtg cggtctccag
     7561 gtcgtgactg agtgcgggca ggctcggctc gatcttggcg aggtaggcgt cgaggtcggt
     7621 gagcatttgg ccgatcttct tgccccggcc gttcacggaa gaggcgatgg caccgagcgt
     7681 ttcgttgagt ttcggcgggt cgatgctcga cagcactgat gtgaggttct cgaacaccgt
     7741 gttgatctcg accatgacgt gtttggccgc cagcacctga ccggcgcgca gcgattcggg
     7801 cgacggatcc ggcgggaaga cgagctggac gtatttcgcg ccgaacaccg tcgtcgacgc
     7861 gatgtcgacg agcacgttcg ccggtatcgc actcattcgt ttggggtcca tcgccagatg
     7921 aagggcggcc tcgccgttgg gcagctgctc gatcgaggcg accttgccca cctgcacgcc
     7981 gcggatctgc accttggcgt ccgggttcat caccaacccg gcccgcggcg acatcaccgt
     8041 cacgggcacg ctgtcggtga ggccaccgcg aaacagggtc gcggacagtg cgaacaccgc
     8101 gatgatcgcg acgacggtgg caacccccat caaggggcgt actgctcgac ccgtcacccc
     8161 gccaccattc gcgctgtcac gcgactcccg gatggatgag tttcaccgtg cccccacagg
     8221 cgtgccgaca cccccgggcc gcgcggcgac tttaccggcg gaacaagtat ttggtcaact
     8281 gtccggggac tggcacagtt gcaacccccg cgccggtatc gatggcgctg cgggtatgcc
     8341 tccatcgatc cccctgaagt gagcggcgtc tgcctggggc gatggctatt atctaaaaat
     8401 agcaataatt ggtcaatagc gtgcgaatgc tcgatgccgt cccgacgggg tgtatcaatc
     8461 ggcgttcgcg ccgccatcgc gacctccctg aggcagccgt tacggattca ccgtaaaaga
     8521 cgcgcatagg gcccgtattc ggaacggcgg atttattaac tgtgcagcgg ctgtgctgac
     8581 ggtgggccgc gatgtcggcg ccgatcaacg agcgtcgcaa ttgcgctttg gccgagttcg
     8641 gacagcctgt aagcatccgc tcgaggatgg cggtgagccc gtaatcggct ccatctcagt
     8701 ggttttcgca ggcgaagtcg tgttgctcgt catcgatgca ttcatagcgg cagtgctttc
     8761 accgcccgct tgtcaggatg gcacacgccg ccactcgagg ttcgtcgaca attcttacgg
     8821 attcggtgaa tttgcgacgg cgcgccttct cgtgtcgcac cggtgcgaca attccgacgt
     8881 cgatgattcg ctagccagtc gtaaccggca gcctcgccca tccccgcaca ctggacgtcc
     8941 gcgctcggct ggcgttgccg tagtcgacgt cccagtcggt ccatcgtttg aggacctctt
     9001 cgagcgcaac gcggccttcg agtcgcgcca gtgccgagcc gaggcagaaa tgcagccctt
     9061 gaccaaagct caggtgacca cccttgcggt gaatgtcgta gcggtccggt tcggcgaatc
     9121 tcgtctcgtc gcggttggcc gaaccgttga ggagcaacat gtacgacccc tcggccaccg
     9181 tgcgtccata gtgctcgacg tcggaggcga cgtagcgcgc ctgcaccggc gacgggggct
     9241 cgtagcggag tgtctcctcc accgccgacg aaatcagcga cgagtccgcg acgagttcgc
     9301 gtcgctggtc ggggtggtcg cccagcagtt gccccatgaa accgatcagc cgagcggtgg
     9361 tctcgttgcc tgcaccggcg atcatcgcgg tgtatgccag taattcggtg cgttcgagac
     9421 ggcgacgggt gccgtcctgc tcttctatct ccgcgttgag cagctcggtc atcagatcgt
     9481 cggaggggtg cgtcgcccgc cactggatgt actcggcgaa catcgcgatc gactcaccga
     9541 tgatcgtctg gctgagatcg ccgtcctcgc cgtgccggga gctgaccgtg atgctcttgt
     9601 cgttgcggtc acggatctgc tgctggcctt cctcggggat gccgaggaga tagccgatcg
     9661 ttcgcatcgg catgatggcg ccgaggtcga caacgaagtc gaaaccgtcg gcgccacgta
     9721 gcggatccaa tgcgcgcgaa cagaattcac gcaccagctc ttccaccgcc agcattcgcc
     9781 gcggggtgaa cacccgtgac aacaggcgcc ggtgcagatc gtgcaggggc ggatcctcga
     9841 acagcaggat tcccgggggc acctcgatgc cgctgaacag aatgtctgcg gtggtgccgc
     9901 ggcccgaccg gtacgtctgc caatccggaa gtgcacgcgc gacgtcgtca tagcgactca
     9961 gcgcgtagaa gttgtacttc tcgttgaagt acaacggtgc ttccgcgcgc atccgtttcc
    10021 acaccgaata cgggtcgtcg tcgatcgcga agtcgaacgg gtcgtagtac acctcgaccg
    10081 tcgggccact
//
LOCUS       DASYLX010000900         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1040979,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000900 DASYLX010000000
VERSION     DASYLX010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040979"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGP27_21025"
     CDS             complement(<1..621)
                     /locus_tag="VGP27_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV7853816.1"
                     /translation="MSETTSRVLQLLGLLQSRRVWSGEQLAERLGVTTRSVRRDVERL
                     RDLGYPVLASKGHGGGYQLAAGAALPPLLLDPDEAVAVAVCLRLAAGGSVAGVGESAL
                     RALSKLDQVMPARLRSQVSAVHEATVTLTWDSSDSPVEPDLLMTLARACRDHEHVLAS
                     YVDRAGNATERRLEPYQLVTTGKRWYLLAFDRDREDWRSLRLDRMHD"
     gene            684..1229
                     /locus_tag="VGP27_21030"
     CDS             684..1229
                     /locus_tag="VGP27_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7853817.1"
                     /translation="MSAKANQKTVSQLADQLDFHWTHQLRPRLDGLTDDEYFWCPVPH
                     CWTVHSDGSIDFEHPAPEPAPFTTIAWRLAHVIVGVLAMRNHSHFAGPPADYQSWPYA
                     TDAATALRQLDDAYQNWIAGVRALGDDELDRACGPAEGPFADYPLMTLVLHINREMIH
                     HGAEIACIRDLYANSPEKKEK"
     gene            1232..1828
                     /locus_tag="VGP27_21035"
     CDS             1232..1828
                     /locus_tag="VGP27_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7853818.1"
                     /translation="MPGMPSPAADERQTLIEFLRFNQNAFFAVAYGLTDDQARSKPSV
                     SALSIGGLVKHATGVQHSWMARVTAAPGSPPRDDRPFDEQAAEYQDQYLMRDDETLAE
                     LLERLRAQNEETLRIFGEADLDAAVPVPHDVPWFPQDLDFWNVRWVAMHLVEELTRHA
                     GHCDIIRESIDGATMYELMAGIEDWPETDWIKPWKKST"
     gene            1850..2782
                     /locus_tag="VGP27_21040"
     CDS             1850..2782
                     /locus_tag="VGP27_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853819.1"
                     /translation="MQTRTGTAPCGDVELFYEDLRPSGSSRARCDPDAPPVLLIMGVG
                     AQLPMWPDGFCAQLVGQGYRVVRFDHRDTGLSTKMHGTKADGSVYRRVLRHVTGRRSE
                     VPYTLVDMADDVRGLLDHLGIERVHVVGASMGGMIAQVLAGNHPDRLASLGLLMSSSN
                     RLLSAVPRWRVIKLAFGGPAKDAPWEERLAAEMRSISIINGPNFLPSADVLRRRVEDL
                     RTRSDYPQGMLRQFDAILGTGSLLRYSRSITAPTIVIHGTDDPMVRPRNGRTLARVIA
                     GARFVVVDGMGHDLPEPVWRPVIEALTENFAMAD"
     gene            complement(2851..4152)
                     /locus_tag="VGP27_21045"
     CDS             complement(2851..4152)
                     /locus_tag="VGP27_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853820.1"
                     /translation="MAAILTEPRGSSMKRVAMASFVGSAIEYYDFYIYGTAAALVFPR
                     VFFPHLGTTMATVASMATFAAAFLSRPIGAAFFGHFGDRLGRKSTLIATLLIMGASTL
                     AVGLVPGAATIGMAAPLILLTLRLAQGFAVGGEWAGAALLSAEYAPAEARGRYGMFTQ
                     MGVGSGLVMSSLLFLVVNQTIGEGSRAFVDWGWRIPFLFSAVLIAIALYVRLSVAETP
                     VFAAQKARQSAPVTPLTALFRNQRREVVLAAGSMIGFFTLGYLANAYFMSYAHNHVGF
                     SPDLILAVGLLGGVIVVVFNALSAMASDIFGRRRVIIAALAVGIPWSFVVMPLIDTGN
                     AAWFALAMSGTYAVAASSYGPMAAFIPEIFGTRYRYSGAGLSLNLAGLVGGAVPTIIA
                     APLLATWGASAIGVMMAAVVLVSLVCTVALPETKGVALQPQ"
     gene            4358..>4551
                     /locus_tag="VGP27_21050"
     CDS             4358..>4551
                     /locus_tag="VGP27_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV7853821.1"
                     /translation="MSTTKHREVAKLDRVPLPVEAARIGATGWQVTRTGARVVTKLAG
                     RGSLQQKVIREIPQTFADLGP"
BASE COUNT          816 a         1562 c         1474 g          699 t
ORIGIN      
        1 atcgtgcatc cggtccagtc gcaggctgcg ccagtcctcg cggtcgcggt cgaacgccaa
       61 tagataccag cgcttccccg tcgtgacgag ctgatagggc tccagccgcc gctcggtggc
      121 gttgccggcg cgatcgacat aggacgccaa cacgtgttcg tggtcgcggc aggcgcgcgc
      181 gagagtcatc agcagatccg gctcgacggg cgaatcggaa gaatcccagg tgagcgtgac
      241 ggtggcctcg tgcaccgccg agacctgtga acgcagccgc gccggcatca cctggtccag
      301 cttgctcagc gcccgcaggg cggattcgcc gacaccggcg acgctgcccc ctgcggccaa
      361 ccgcaggcac accgccaccg ccaccgcctc gtcgggatcc agcagaagcg gtggcagcgc
      421 ggctcccgct gccagctgat agccgccgcc gtggcccttg ctcgcgagca ccggatagcc
      481 caggtcacgc agcctttcga catctcggcg cacgcttcgg gtggtcacac cgagccgctc
      541 ggcgagctgc tcccccgacc acacccgccg cgactgaagc agacctagca gttgcaagac
      601 ccggctcgtc gtctcggaca cgttttccag actcgcacag tcataggaca gaaactgtcc
      661 tctatggatg agaggttggt gtcatgagtg cgaaagcgaa ccagaaaaca gtcagccaac
      721 tcgccgacca gctcgacttt cactggaccc accagctccg gccacggctc gacgggctga
      781 ccgatgacga gtacttctgg tgcccggtgc cgcactgttg gaccgtgcat tccgacggtt
      841 cgatcgactt cgaacacccg gcgcccgagc ccgcaccgtt caccaccatt gcgtggcggc
      901 tcgcgcacgt catcgtcgga gtgctggcga tgcgcaacca ctcgcacttc gccgggccac
      961 ccgcggacta ccaaagctgg ccgtacgcca ccgacgcggc tactgcgctg cgtcagctcg
     1021 acgacgccta ccagaattgg atcgccggcg tgcgcgccct gggcgacgac gagcttgatc
     1081 gggcctgcgg acccgccgaa ggaccgtttg ccgactaccc gttgatgacg ctggtcctgc
     1141 acatcaaccg ggagatgatt caccacggcg ccgaaatcgc ctgcatccga gacctttacg
     1201 ccaacagccc cgagaagaag gagaagtagc cgtgccaggt atgccctcac ccgccgccga
     1261 cgagcgccag acgttgatcg agtttctccg gttcaatcag aacgcattct tcgcagtcgc
     1321 ctatgggctg accgacgatc aggcccggtc caagccgtcg gtgagcgcgc tgtcgatcgg
     1381 cggactcgtc aaacacgcca ccggtgtgca gcacagttgg atggcgcgcg tgacggcagc
     1441 gcctggttcg ccgccgcgcg acgaccgacc gttcgacgag caggcggccg aataccagga
     1501 ccagtacctg atgcgcgacg acgaaacgct tgccgaactg ctcgagcggc tacgcgcgca
     1561 gaacgaggag acgctacgga tcttcggaga agccgacctc gacgccgcgg tccccgttcc
     1621 gcatgatgtg ccgtggttcc cgcaggatct ggacttctgg aacgtgcgct gggtggcgat
     1681 gcatctcgtc gaggagctga cccggcacgc cggtcactgt gacatcatcc gcgaatccat
     1741 cgacggggca acgatgtacg aattgatggc cggcatcgag gactggccgg aaaccgactg
     1801 gatcaagccg tggaagaagt cgacgtagcg gcacggtaga aaggactccg tgcagacccg
     1861 aacgggcacc gcgccctgcg gcgacgtgga gttgttctac gaggaccttc gcccgtccgg
     1921 ctcctcgcgt gcgcggtgcg atcccgacgc cccgcccgtg ttgctgatca tgggcgtcgg
     1981 cgcccagttg ccgatgtggc cggacggatt ctgcgctcag ttggtcgggc agggctatcg
     2041 ggtcgtccgg ttcgaccacc gcgacaccgg cctgtcgacc aagatgcacg gcacgaaggc
     2101 cgacggctcg gtgtaccggc gagtgctgcg ccacgtgacg ggtcgccgca gcgaagtgcc
     2161 ttacacactc gtcgacatgg ccgacgacgt gcgaggcctg ctcgaccacc tcggcatcga
     2221 gcgagtccac gtcgtcggcg catcgatggg cggaatgatc gcgcaggtgc ttgcgggcaa
     2281 ccatcccgac cggctggcgt cgctcgggct gctgatgtcc agttcgaacc gattgctctc
     2341 ggcggtgccg cggtggcggg tcatcaagct ggccttcggc gggcccgcga aagacgcgcc
     2401 gtgggaggag cggctggccg ccgaaatgcg cagcatctcg atcatcaacg gccccaactt
     2461 cttaccgtcg gcggatgtgc tgcggcgccg cgtcgaagat ttgcgcaccc gaagcgatta
     2521 cccgcagggc atgctgcgtc agttcgacgc catcctcggc acgggcagcc tgttgcgcta
     2581 cagccgcagc atcaccgcgc cgacgatcgt catccacggc accgacgacc cgatggtgcg
     2641 gccgcgcaac ggccgcaccc tggccagggt gatcgcgggc gcgaggttcg tcgtcgtgga
     2701 cggtatgggc cacgacctgc cggagccggt gtggcgcccc gtcatcgagg cactcaccga
     2761 gaacttcgcc atggccgact gactgctagc cgggcagacc ttgctcctcg gacggcggcg
     2821 tcgtgcggtg cttgcattcg cgcctgccgc tcactgtggt tgcagcgcaa cgcctttggt
     2881 ctcgggcagt gcgacggtac acaccaggct gacgagcacg acggccgcca tcatgacgcc
     2941 gatcgcggac gcgccccatg tcgccagcag tggcgcggcg atgatcgtcg gcaccgcgcc
     3001 gccgaccagt ccggcgaggt tgagcgacag gcccgcaccg ctgtagcggt accgcgtgcc
     3061 gaagatctcg gggatgaacg ccgccatcgg cccgtatgag ctcgcggcga cggcatacgt
     3121 gcctgacatc gccagcgcga accaggccgc attgccggtg tcgatcagcg gcatgacgac
     3181 gaacgaccat ggaatgccca ccgccaacgc cgcgatgatg actcggcggc gaccgaagat
     3241 gtcgctggcc atcgcgctca gcgcgttgaa caccaccacg atcacaccgc ccagcagccc
     3301 caccgccaga atcaggtccg gcgaaaaccc cacgtggtta tgggcataac tcatgaagta
     3361 agcgttggcc aggtagccga gggtgaagaa gccgatcatg ctgcccgcag cgagaaccac
     3421 ctcgcgccgc tgattgcgga agagcgcggt cagcggtgtc accggcgcgc tctggcgcgc
     3481 cttctgtgcg gcgaacaccg gcgtctcggc cacgctcagc cgcacgtaca gcgcgatggc
     3541 gatgagcacc gcactgaaca ggaacggaat ccgccagccc cagtcgacga aggctcgact
     3601 gccttctccg atcgtctggt tcaccaccag gaacagcagg ctgctcatca cgaggccgct
     3661 gccgacgccc atctgggtga acatcccgta gcggccacgg gcttcggctg gtgcgtactc
     3721 ggcggacaac aatgcggcgc ccgcccattc gccgccgacc gcgaacccct gcgcgaggcg
     3781 cagcgtcagc aggatcagcg gcgcggccat gccgatcgtc gccgcaccgg gcaccagccc
     3841 caccgccagc gtggaggcgc ccatgatcag cagggtggcg atcagggtcg acttgcgacc
     3901 gagccggtcg ccgaagtggc cgaagaacgc cgcaccaatc ggccgggaca gaaacgccgc
     3961 ggcgaacgtg gccatcgacg cgacggtcgc catggtcgtg ccgagatgcg ggaagaacac
     4021 cctgggaaac accagcgccg ccgcggtgcc gtaaatgtag aagtcgtagt actcgatcgc
     4081 ggacccgacg aagctcgcca tggcgacgcg cttcatcgag ctgccccgcg gttcggtcag
     4141 tatcgctgcc atcttcgctc ctcggacgga ttggtttcat ccgaagctat ggagctgcgg
     4201 cggcactggc tcgcttgtgt gcactacccg cggtatccag cgatcttgag gttgtgcgca
     4261 ttgtgctcaa cggggcgcgg ccgccacaat cgcaggtcac tgtgaccggc tccacaggtt
     4321 ttcgcgggtc acggcggatt ttgagagact gctagctatg agtacgacta agcaccgcga
     4381 ggtggccaag cttgaccggg tcccgttgcc cgtcgaggcc gcccgcatcg gagcgacggg
     4441 ttggcaggta acccgcaccg gcgcacgggt cgtcaccaag ctcgccggcc gcggctcact
     4501 gcagcagaag gtcatccggg agattccgca gacgttcgcc gacctcggcc c
//
LOCUS       DASYLX010000901         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2358955,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000901 DASYLX010000000
VERSION     DASYLX010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358955"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="priA"
                     /locus_tag="VGP27_21055"
     CDS             <1..715
                     /gene="priA"
                     /locus_tag="VGP27_21055"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEV7853822.1"
                     /translation="VDGKAVRLVQGQAGSETDYGSALDAAMTWQRDGAEWIHLVDLDA
                     AFGRGSNRELLAEVIGKLDVAVELSGGIRDEESLSAALATGCARVNLGTAALENPEWC
                     AQAIAEHGDKVAVGLDVKIDPSVPGGGFRLRGRGWETDGGDLWPVLDRLDSEGCSRFV
                     VTDVTKDGTLQGPNLGLLSTVAERTDAPVIASGGVSSLDDLRAIATLTDSGVEGAIIG
                     KALYAGRFTLPQALAAVGE"
     gene            723..1541
                     /locus_tag="VGP27_21060"
     CDS             723..1541
                     /locus_tag="VGP27_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEV7853823.1"
                     /translation="MALDETDIEALVSAASAILDEAAKPFVSGHGAESAVKKKGDDFA
                     TEVDLAIERQVVEALVSSTGIEVHGEEFGGADINSPLVWVLDPIDGTFNYAAGLPTAA
                     ILLALVRDGQPIAGLTWLPFTDQRYTAVVGKPLYANNVAQPPLKSAGVDASVVGTGTF
                     DVDSRGRFPGRYRLAIVEQLSRRCSRMRMHGAIGVDLAYTAAGILGGAITFGGHVWDH
                     AAGVALVRAGGGVVTDLAGNDWTTDSKSALAGVPGVHDELLDIVRSAGDPDDYR"
     gene            1538..2320
                     /gene="hisF"
                     /locus_tag="VGP27_21065"
     CDS             1538..2320
                     /gene="hisF"
                     /locus_tag="VGP27_21065"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV7853824.1"
                     /translation="MNPKDLAVRIIPCLDVDDGRVVKGVNFENLRDAGDPVELAAAYD
                     REGADELTFLDVTASSSGRATMLEVVKRTAEQVFIPLTVGGGVRSVADVDVLLRAGAD
                     KVAVNTAAIARPELLAELSRKFGSQCIVLSVDARTVPADTEPTPSGWEVTTHGGRRGT
                     GIDAIEWAARGAELGVGEILLNSMDFDGTKAGFDLRMLRAVRGAVSIPVIASGGAGAK
                     EDFAPAVVAGADAVLAASVFHFGELTIAEVKAAMAAEGVVVR"
BASE COUNT          381 a          730 c          848 g          385 t
ORIGIN      
        1 cgtcgacggc aaggccgtgc gtctcgtgca gggccaggcc ggcagcgaaa ccgactacgg
       61 ctccgcactg gacgccgcga tgacctggca gcgcgacggg gcggagtgga tccatctggt
      121 ggacctcgac gccgcgttcg gccgcggatc aaaccgcgag ctgctcgccg aggtgatcgg
      181 caagctcgac gtcgccgtcg agttgtccgg cggcatccgc gacgaggagt cgctgtcggc
      241 cgcgctggca accggctgcg cccgggtcaa cctcggcact gcggcgctgg aaaaccctga
      301 gtggtgcgcg caagcgatcg cggagcacgg cgacaaggtg gcggtcggac tcgacgtaaa
      361 gatcgacccc tccgtgcctg gtggcgggtt ccgactccgc ggccgcggct gggagaccga
      421 cggtggtgat ctgtggccgg tgctggatcg tctggacagc gagggctgtt cgcgattcgt
      481 cgtgacagat gtcacgaagg acggcacgct gcaaggaccc aacctcggtc tgctgtccac
      541 cgtcgccgaa cggacagacg ctccggtcat cgcgtccggt ggggtgtcca gccttgacga
      601 tctgcgtgcc atcgcgaccc tgaccgacag cggcgtcgaa ggcgcaatca tcggaaaagc
      661 gctgtacgcc gggcgattca cgctgccgca ggcgcttgcc gcggtagggg agtagcccgg
      721 ccatggcgct cgacgagacc gatatcgaag cgctggtctc tgcggcctcc gcgatcctgg
      781 acgaggcggc caagccgttc gtgtccggcc acggtgccga atctgcggtg aaaaagaaag
      841 gcgacgactt cgccacagag gttgatctcg ccatcgaacg gcaggtggtc gaggcgctgg
      901 tgtcgagcac cggtatcgag gtgcacggcg aagagttcgg tggcgccgac atcaattcac
      961 cgctggtgtg ggtgctcgac ccgatcgacg gcacgttcaa ctatgcggcc ggtctgccga
     1021 cggcggcaat cctgctggcc ctcgtccgag acggccagcc gatcgcgggt ctgacatggt
     1081 tgccgttcac cgatcagcgg tacacggcgg tggtcggaaa gccgttgtac gccaacaatg
     1141 tcgcgcagcc accgctgaag tcagccggcg tggatgccag tgtggtcggc acgggtacct
     1201 tcgatgtcga ctcgcgggga cgcttcccgg gccgctatcg cctcgcgatc gtcgaacagc
     1261 tgagccgacg atgctcgcgg atgcgcatgc atggcgccat cggcgtcgac ctcgcgtaca
     1321 ccgccgccgg aatcctcggc ggcgccatca ccttcggtgg tcatgtgtgg gatcacgcgg
     1381 ccggcgtggc cttggtgcgg gctggcggag gcgtcgtcac cgatctggcg ggcaacgact
     1441 ggacgaccga ttccaagtcg gcactggcgg gcgtacccgg tgtacacgat gagctgctcg
     1501 acatcgtgcg ctcagccggc gatccggatg actaccgatg aatccgaagg acctcgcggt
     1561 tcgtatcatt ccgtgcctcg acgtcgacga cggccgggtg gtcaagggcg tcaacttcga
     1621 aaatttgcgt gacgcaggag atcccgtcga gctggcggcc gcgtacgacc gcgagggcgc
     1681 cgacgagctg accttcttgg atgtgacggc gagttcgtcc ggtcgcgcca ccatgctgga
     1741 ggtcgtcaaa cggaccgcgg agcaggtttt cattccgctc accgtcggcg gaggcgtgcg
     1801 gtcggtggca gatgtcgacg tgctgctgcg tgcgggcgcc gacaaggtcg ccgtcaacac
     1861 ggcggccatt gcgcgccctg aactgctggc cgaactgtcc cgcaagttcg ggtcgcaatg
     1921 catcgtgttg tcggtcgacg cccggaccgt gccggcggat acggagccga cgccgtcggg
     1981 atgggaggtc accacccacg gcgggcggcg cggtaccggc atcgacgcga tcgaatgggc
     2041 cgcccgcggt gcagaactgg gcgtcggcga gattctgctc aactcgatgg acttcgacgg
     2101 caccaaggcc ggattcgacc tcagaatgct acgggccgtg cgcggtgcgg tgagcatccc
     2161 ggtgatcgcc agcggtggcg ccggtgccaa ggaagatttt gctcccgcgg ttgttgcagg
     2221 agccgacgcc gtgctggccg ccagcgtgtt ccacttcggg gagctgacga tcgccgaggt
     2281 gaaggcggcg atggcggcgg aaggggtcgt ggtgcgatga gcgcttgcgc gaagagcagt
     2341 aggc
//
LOCUS       DASYLX010000902         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2687059,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000902 DASYLX010000000
VERSION     DASYLX010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687059"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGP27_21070"
     CDS             complement(<1..527)
                     /locus_tag="VGP27_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV7853825.1"
                     /translation="MAGNRVALALGSGGARGYAHIGVITELHARGYEIVGIAGSSMGA
                     LVGGLEAAGKLDEFADWAKALTQRAVLRLLDPSIAAAGVLRAAKILDAVRDILGDVTI
                     EDLPIPYTAVSTDLIAGKSVWLQRGPVDAAIRASIAIPGVITPHILDGRLLADGGILD
                     PLPMAPIAAVNADLT"
     gene            complement(649..1074)
                     /locus_tag="VGP27_21075"
     CDS             complement(649..1074)
                     /locus_tag="VGP27_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqV"
                     /protein_id="HEV7853826.1"
                     /translation="MRFYLLCVAATVTALAVAGCSSGGEGGQTSAAPTTTAATSSETH
                     TPAPGAVEIAPGGATTAVGADAESTEEEYSQACQAAKVWIDQQGGDRKSQVEPLLKML
                     QDPNTPPGPATYNTPWAQLSPARQAAVIVAVQAGADSLC"
     gene            1188..1745
                     /locus_tag="VGP27_21080"
     CDS             1188..1745
                     /locus_tag="VGP27_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEV7853827.1"
                     /translation="MVSALSVPSPAASPARSAPTRLRVPDLLQAVDQAADGVLSGRYD
                     HLLPADGLPVDDRWYTRIHGDGELDVWLISWVPDHATELHDHSGSLGALTLVSGALDE
                     FRWDGEALRRRRLNAGDQAAFPLGWVHDVVWAPGPAPAPAAPATPTLSVHAYSPPLTA
                     MSYYEVTDRNILRRNRTELTDGPEG"
     gene            1753..2127
                     /locus_tag="VGP27_21085"
     CDS             1753..2127
                     /locus_tag="VGP27_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7853828.1"
                     /translation="MASRIDKVLDAARARLDRMPAKAVPGALERGAVLVDIRPQAQRD
                     REGHVPAALVVERNVLEWRCDPTSDAKLPEAVGDDVEWVVLCSEGYTSSLAAASLIDL
                     GLHRSTDVVGGYHALKAEGVLV"
     gene            complement(2158..3858)
                     /locus_tag="VGP27_21090"
     CDS             complement(2158..3858)
                     /locus_tag="VGP27_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEV7853829.1"
                     /translation="MIDSAEATEEAPPQHEPPGKKTTWWQRHYTFTGTVLAVVFLWLS
                     LTPSLLPRGPLFQGLVSGAAGAIGYGIGVFLVWLVQYMLSKDASPHAPGWAWLAVVVV
                     GVIGQILMIIYFHVWQDEVRDLNGVPRMTFWDHPLTGVLAILVLFIFVEIGQLIGKLV
                     RFLVRQLNRFAPPRVSAVIVLVLLLALSIALLNGVVVRVGMNYLNKTFAAVNDETDPD
                     FPAPMSPLRSGGPQSLASWESLGHQGRVFVSAGPTVQELTKFNGSPATEPTRAYAGLH
                     SADGIKATAALAAEELRREGGLNRDVVAVATTTGTGWINEAEASALEYMYNGNTAIVS
                     MQYSFLPSWLSFLVDKENARQAGQALFEAVDALIREVPEGQRPKLVVFGESLGSFGGE
                     APFLSLNNLVARTDGALYSGPTFNNTIWADLTHNRDKGSPEWLPMFDKGENVRFVARP
                     DNLGRPADPWGKPRAVYLQHASDPIAWWNPDLLFQEPDWLREPRGYDLSGRMQWIPIV
                     TFLQVSADMAVAVDVPDGHGHVYVKDVANGWAAVLQPPGWTPEKTERLRPLLSNDENA
                     "
     gene            complement(3868..>4360)
                     /locus_tag="VGP27_21095"
     CDS             complement(3868..>4360)
                     /locus_tag="VGP27_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7853830.1"
                     /translation="TPLIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLP
                     GMGGSQRLTRAVGKAKAMDLILTGRTIDAEEADRSGLVSRVVPADELIREANAVATTI
                     SQMSLSATRMAKEAVNRAFESSLAEGLLYERRLFHSAFATADQTEGMNAFSEKRPPNF
                     THS"
BASE COUNT          705 a         1468 c         1475 g          712 t
ORIGIN      
        1 atggtgaggt cggcgttgac ggccgcgatc ggcgccatcg gcaggggatc gaggatcccg
       61 ccgtcggcga gcagacggcc gtcgaggatg tgcggtgtga tgacgcccgg aatggcgatg
      121 gatgcccgga tcgccgcatc gacgggacca cgctgcagcc agaccgactt gccggcgatc
      181 aggtccgtcg acacggccgt gtaggggatc ggcaggtctt cgatggtgac atcgccaagg
      241 atgtcgcgga ctgcgtcgag aatcttcgcc gcgcgcagca ctccggcggc cgcgatcgac
      301 gggtccagta ggcggagcac agcgcgctgg gtcaacgctt ttgcccagtc ggcgaattcg
      361 tcgagcttgc ccgccgcctc caaaccaccg acaagcgcac ccatcgacga cccggcgatc
      421 ccgacgatct cgtagccgcg ggcgtgcagt tcggtgatca cgccgatgtg ggcgtaaccc
      481 cgtgcacccc cgctgccgag agccagcgcc acgcgattgc cagccatgcg tccattctgc
      541 gccgtcgtcg tactgcgcat gcgcgtgctg gacgaggcgg tcgcgcgggg ccgggactgg
      601 atcgcgagcc gcagatagcc gactttctcg caaccgtgat tcgaattgtc agcagagcga
      661 gtccgcgccc gcttggaccg cgacgatgac ggccgcttgg cgggcagggg agagctgcgc
      721 ccacggggtg ttgtaggtgg ccggaccggg cggtgtgttg ggatcctgca acatcttcag
      781 caacggctcc acctgcgact tccggtcgcc gccttgctgg tcgatccaga ccttcgcggc
      841 ctggcaggcc tgcgagtact cctcctcggt cgactcggcg tccgccccga cggctgtggt
      901 cgcgccgccc ggcgcaatct cgacggcgcc gggtgccggg gtgtgggttt ccgagctggt
      961 cgcggccgtt gtggtggggg ccgccgacgt ctgaccgccc tcgccgcccg acgaacagcc
     1021 tgcaaccgcg agagccgtca ccgtggccgc gacgcaaagg aggtagaagc gcatgctgcc
     1081 aatctatgca acactcgcgg acgtgatgga ggggatctgg tctcggtggt gttgtccggc
     1141 gtagccgccg ccccccgacc cgtctgtctg tcacttctgg agtgttcatg gtctccgctc
     1201 tttctgtacc ctcccctgct gcttcgcccg cccgctccgc ccccacccgc ctgcgcgtcc
     1261 ctgacctgct gcaggccgtc gaccaagcgg ccgacggcgt gctcagcggc cgctacgatc
     1321 atcttctgcc cgcggacggg ctgcccgtcg acgaccgctg gtacacccgc atccacggcg
     1381 acggcgaact ggacgtctgg ctcatcagtt gggtccccga ccacgccacc gagctgcacg
     1441 accacagcgg ctcactcggt gcgctgacgc tggtgtccgg cgcactggac gaattccgtt
     1501 gggacggtga ggccttgcgg cgccggcggc tcaatgcagg cgaccaggcg gcctttccgc
     1561 ttggctgggt gcacgacgtc gtatgggcgc caggaccggc gcctgcgcct gccgctcccg
     1621 ctacgcctac gctgagcgtg cacgcctact cgccgccgtt gacggcgatg tcgtattacg
     1681 aggtgaccga ccggaacatc ctgcgtcgca accgaaccga actgaccgac ggcccggagg
     1741 gatgaagaac tgatggccag ccgtatcgac aaggtgctcg acgccgcccg cgcccggctc
     1801 gatcggatgc ccgccaaggc cgtgcccggt gcactggagc gcggcgcggt gctcgtcgac
     1861 atccgtccgc aggcgcagcg cgaccgcgag ggacacgtcc ccgcggcact cgtcgtcgaa
     1921 cgcaatgtgc tggagtggcg ctgcgatccg accagtgacg ccaaactgcc ggaggccgtc
     1981 ggcgacgacg tggaatgggt ggtgctgtgc tcggagggct atacctccag tctggccgcg
     2041 gcgtcgctga tcgatctcgg gttgcacagg tcgaccgacg tggtcggcgg gtatcacgcg
     2101 ttgaaggccg aaggcgttct ggtttagcgc acgcgagggc ccgaggagcg agagaggtta
     2161 agcgttctcg tcgttggaca gcagcgggcg caggcgctcg gtcttctccg gcgtccatcc
     2221 cggcggctgc agcacagcgg cccagccgtt cgcgacgtcc ttgacgtaga cgtggccgtg
     2281 cccgtcgggg acgtcgaccg ccaccgccat gtcggcggac acctgcagga acgtgacgat
     2341 cggaatccac tgcatccgcc cggacaggtc gtagccgcgc ggctcgcgta gccagtccgg
     2401 ttcttggaag agcaggtcgg ggttccacca cgcgatcgga tcggacgcgt gctgaagata
     2461 gaccgcacgc ggcttgcccc acgggtcggc ggggcggccg agattgtccg gtcgcgcgac
     2521 gaagcggacg ttctcgccct tgtcgaacat cggcagccac tccggcgatc ccttgtcacg
     2581 gttatgggtg agatcggccc agatcgtgtt gttgaacgtc ggaccggagt agagggcgcc
     2641 gtcggtgcgg gcgacgagat tgttcagcga caggaacggc gcctcgccgc cgaacgagcc
     2701 aaggctctcg ccgaacacga cgagcttggg ccgctgcccc tccggcacct cccggatcaa
     2761 cgcatccacg gcctcgaata acgcctgacc ggcctgtcgg gcgttctcct tgtcgacgag
     2821 gaacgacagc cagctcggca ggaacgaata ctgcatggac acgatcgcgg tgttgccgtt
     2881 gtacatgtac tccagcgccg atgcctcggc ctcgttgatc cagccggtgc cggtcgtggt
     2941 ggccaccgcg accacatcgc ggttcaggcc gccctcgcgc cgaagctctt ctgcggcaag
     3001 cgccgcggtc gctttgatgc cgtcggcgga atgcaagccc gcatacgcgc gggtcggctc
     3061 ggtcgcgggg ctgccgttga acttcgtcag ctcctggacc gtcggtcccg ccgacacgaa
     3121 caccctgccc tggtgtccca gcgactccca gctggccagc gactgcggcc cgccggagcg
     3181 cagcggcgac atcggcgccg ggaaatccgg atccgtttcg tcgtttaccg cggcgaaggt
     3241 cttgttcagg tagttcatcc cgacgcggac gacgacaccg ttgagcagcg cgatgctcag
     3301 cgcaagcagc aagaccaaaa cgatgaccgc cgaaacgcgt ggtggggcaa agcgattcag
     3361 ttgccgaaca aggaagcgca cgagcttgcc gatgagctga ccgatctcga cgaagatgaa
     3421 cagcacgaga atagcgagca ctccggtcag cgggtggtcc caaaacgtca tccgcggcac
     3481 accgttgagg tcgcgcacct cgtcctgcca gacgtggaag tagatgatca tcaggatctg
     3541 gccgatgacg ccgacgacga ccacagccag ccacgcccag cccggcgcgt gcgggctggc
     3601 gtccttcgag agcatgtact gcaccagcca gacgaggaag acgccgatcc cgtagccgat
     3661 cgcgcccgcc gcgccgctga ccagtccctg gaacagtgga ccgcggggca gcagggatgg
     3721 cgtcaacgac aaccaaagga acaccacagc caggacggtc ccggtgaacg tgtagtgccg
     3781 ttgccaccag gtcgtcttct ttcccggtgg ttcgtgctgc ggtggtgctt cttccgtcgc
     3841 ctccgcgctg tctatcacga aggcagatta gctgtgggtg aagttcggcg ggcgtttctc
     3901 gctgaaggcg ttcatgccct cggtctggtc ggccgtggcg aacgccgagt ggaacagcct
     3961 gcgctcgtac agcaggccct cagccaacga cgactcgaag gcgcggttga cggcctcctt
     4021 ggccatccgc gttgccgaca gcgacatctg agaaatggtg gtggcaacgg catttgcctc
     4081 gcggatcagc tcgtcggcgg gcacgacgcg cgacaccagg ccgctgcggt cggcttcctc
     4141 ggcatcgatg gtgcgaccgg tcaggatgag atccatcgcc ttggccttgc cgacggcacg
     4201 ggtcagccgt tgcgagccac ccatgcccgg aagcacgccg agcttgatct cgggctggcc
     4261 gaacttggcg gtgtccgcgg cgatcagcac gtcgcacatc atcgccagct cacagccgcc
     4321 gccgagtgcg taaccggcga ccgcggcgat cagcggcgtg
//
LOCUS       DASYLX010000903         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3456087,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000903 DASYLX010000000
VERSION     DASYLX010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3456087"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP27_21100"
     CDS             complement(<1..351)
                     /locus_tag="VGP27_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid C27-monooxygenase"
                     /protein_id="HEV7853831.1"
                     /translation="MTPSAPRVRQAAPNLPPGFDFTDPDVYAERLPTEEFAEVRRAAP
                     IWWNEQQRDVGGYGDGGFWVVSKHRDVREVSLRSDVFSSAAKTVVPHFKPSVDVKGQI
                     KASRLSMLMMDDPEH"
     gene            442..1383
                     /locus_tag="VGP27_21105"
     CDS             442..1383
                     /locus_tag="VGP27_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853832.1"
                     /translation="MDDGVQSFMRAADEGFPDLSTMTAQQARAFLAGRRLAATNLGDV
                     ASADDRTIAGQDGDVPVRVYRPHGDSVPRPAVVFAHGGGFVLCDLDSHDGFCRAMSRF
                     TETVVVSVDYRLAPEHRAPAAVQDVFAAYCWVTEHADELGVDSSRVLVAGDSAGGNLA
                     AVTTLMCREQSGLMPAGQVLIYPVIEPFFDTESYRKYSTGYVNTRDAMQFYWRQYLDD
                     KLPSPEYLVAPGRAETHEGLPPAVVVTAGFDVLHSEGVAYAQRLRTAKVPVVHRDYPG
                     LFHGFLTMMPFGAGAAARELLWADMRRLLAVDERATA"
     gene            1380..>2674
                     /locus_tag="VGP27_21110"
     CDS             1380..>2674
                     /locus_tag="VGP27_21110"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7853833.1"
                     /translation="MSSDVIVIGAGFAGLYAVHRCLSDGLTVRGVEAAPSVGGTWYWN
                     RYPGARCDVESVDYSYSFDEDLQSEWTWTERFAAQPEILAYLDHVADRFDLRRHYQFN
                     TEVVGAQFDGGEWALQTSDGQSRRARFLMCATGCLSAVNRPDIAGLGDFVGQVYYTAA
                     WPREDPHLRGEKIGVIGTGSSGIQVTPILAEQAASLVVFQRSPNYTIPMPNYPWSDED
                     TERIREEYPQRRQRSAYAPAGTPHGTYHKNALETDPEEREEALWKRWRDGGVLFSKTF
                     PDQGSVLEANDIARRFAEDRIRDIVDDPAVAADLIPTDHPIGTKRICTDAGYYATFNR
                     DNVRLVNLRREPIEAVTPDGVRTSEATYECDVLVFATGFDALTGAMTRIDPVGPDGDR
                     LSALWADGPVTFLGVMVPRLPNLFSFSGPGSPSVLSNMVL"
BASE COUNT          420 a          838 c          965 g          451 t
ORIGIN      
        1 gtgctcgggg tcgtccatca tcagcatgga cagcctggag gccttgatct gtcccttgac
       61 gtccaccgac ggcttgaagt gcgggacgac cgtcttggcc gccgacgaga acacgtcgct
      121 gcgcagcgat acttcgcgga catcgcggtg cttgctgacc acccagaagc cgccgtcgcc
      181 gtacccgccc acatcgcgtt gctgctcgtt ccaccagatc ggtgcggcgc ggcggacctc
      241 cgcgaactcc tccgtcggca gccgttcggc gtagacgtcg ggatcggtga agtcgaagcc
      301 gggcggcagg ttgggagcgg cttggcggac gcgaggagca gatggggtca tgcggacgcg
      361 aggagcagat ggggtcatgc ggacctcctc aggatggctt tcgtgatggt agccagcgat
      421 cgtgcattac ggttgccgat catggacgac ggcgtgcaga gcttcatgcg cgccgccgac
      481 gagggattcc ccgacctgtc gaccatgacc gcccagcaag cgcgggcgtt cctggccggt
      541 cggcgcctcg ctgccaccaa cctcggcgac gtggcgagcg ccgacgatcg cacgattgca
      601 ggccaggacg gtgacgtgcc ggtgcgcgtc taccgcccgc acggcgacag cgttccgcgg
      661 ccggcggtgg tgttcgcgca cggcggcgga ttcgtgctgt gcgatctcga ttcgcacgac
      721 gggttctgca gggcgatgtc gcgattcacc gagacggtgg tcgtgtcggt cgactatcgg
      781 ctggcaccgg agcaccgcgc gcctgcggcc gtgcaggacg tgttcgcggc gtactgctgg
      841 gtgacggagc acgccgacga actcggcgtc gactcgtcgc gggtgttggt cgccggggac
      901 agcgcgggcg gcaacctcgc cgcggtcacc acgctgatgt gccgagagca gagcggcctc
      961 atgcccgcgg ggcaggtgct gatctatccg gtcatcgaac cgttcttcga caccgagagc
     1021 tatcgcaagt actcgaccgg atacgtcaac acccgcgatg cgatgcagtt ctactggcga
     1081 cagtacctcg acgacaagct gccgtctccg gaatatctgg tggcgccggg gcgggcggaa
     1141 acgcacgagg gtctgccgcc cgcggtcgtc gtgacggccg ggttcgacgt gctgcacagc
     1201 gaaggcgtcg cgtatgcgca gcggctgcgg acggcgaagg tgcctgtggt gcatcgtgac
     1261 taccccggcc tgtttcacgg cttcctgacg atgatgccgt tcggcgcggg ggctgcggcg
     1321 cgcgaattgc tgtgggctga catgcggcgg ctgctcgccg tggacgagag ggcgacggcg
     1381 tgagcagcga cgtgattgtg atcggcgcgg gcttcgccgg gctgtatgcg gtgcatcggt
     1441 gcctttcgga tggcctgacg gtgcgcggcg tcgaggcggc gccgagcgtc ggcggcacct
     1501 ggtactggaa tcgctatccc ggcgcccgct gcgacgtgga aagcgtcgac tactcatact
     1561 ctttcgacga ggacctgcag agcgaatgga cgtggacgga gcggttcgcc gcgcagccgg
     1621 agattttggc gtacctcgac cacgtggcgg accggttcga tctgcgccgt cactaccagt
     1681 tcaacaccga ggtggtcggt gcgcagttcg acggcggcga gtgggccctg cagacgtcgg
     1741 atggccagag ccgtcgcgcc cggttcctga tgtgcgcgac gggttgcctg tcggcggtga
     1801 accggcccga cattgccgga ctcggcgact tcgtgggcca ggtgtactac accgctgcgt
     1861 ggccgcgtga ggatccgcac ctgcgtggag agaagatcgg tgtgatcggc acgggatcgt
     1921 cgggtatcca ggtgacgccg atcctcgccg agcaggccgc gtcattggtg gtgtttcagc
     1981 gctcgcccaa ttacacgatc ccgatgccga actacccgtg gtcggacgag gacaccgagc
     2041 gcatccgcga ggagtatccg cagcgcaggc agcgctcggc ctacgcgccc gccggcacac
     2101 cgcacggcac gtatcacaag aacgcgctcg agaccgaccc agaagagcgc gaagaggcct
     2161 tatggaagcg gtggcgcgac ggcggggtgt tgttcagcaa gacgttcccc gaccagggtt
     2221 cggtgctcga ggccaacgac atcgctcggc ggttcgcgga ggaccgcatc cgtgacatcg
     2281 tcgacgatcc ggccgtcgcc gcggacctga tccccaccga ccatccgatc ggcacgaagc
     2341 gcatctgcac cgatgcgggc tactacgcga cgttcaaccg cgacaacgtg cggctggtga
     2401 acctgcgccg cgaaccgatc gaggccgtca cgccggacgg tgtgcgcacg agcgaggcga
     2461 cgtacgaatg cgatgttctg gtgttcgcaa cgggtttcga cgcactcacg ggtgcgatga
     2521 cgcgcatcga cccggtgggt ccggatgggg atcggttgtc ggcgctatgg gcggacgggc
     2581 cggtgacgtt cctcggggtg atggtgccgc ggctgcccaa cctgttcagc ttcagcggac
     2641 cgggcagccc gtcggtgttg tcgaacatgg tgct
//
LOCUS       DASYLX010000904         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3235471,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000904 DASYLX010000000
VERSION     DASYLX010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235471"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGP27_21115"
     CDS             complement(<1..761)
                     /locus_tag="VGP27_21115"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEV7853834.1"
                     /translation="MPEPTILLLSTSDTDLIAARSSGARYRWANPSRLVDGELTEQID
                     GADLVVVRILGGYRSWQDGIDTVIGSGVPTVVVSGEQAPDAELMSHSTVPAGAAVQAH
                     IYLAQGGMDNLRQLHAFLCDTVLMTGFGFAPPAIIPNWGVLERASTNGDGPTIAVLFY
                     RAQQLAGNTGYVQALCAAIEDAGGRALPVYCASLRTAESDMLDLLKSADAMVTTVLAA
                     GGAVPAAVSAGGNDDTWNVAHLAALDIPILQGLCLT"
     gene            complement(1011..1994)
                     /locus_tag="VGP27_21120"
     CDS             complement(1011..1994)
                     /locus_tag="VGP27_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7853835.1"
                     /translation="MGALKRGMWTSLVAAVCAAVVTACGLGSGGAVPFMVAPGSIKPN
                     PALAGVKITVGSKEYTEQVIMGYILEYTLAAAGAEVRDLTGIVGSRSTREAQLRGQVD
                     VAYEFTGNAWINYLGHEKPIPDSREQYEAVRAEDLKRNDMVWLPPGPMDDTYALAASK
                     AVVERTGVRTLSQYADLVKKNPAAAKTCVDTEFRARQDGYPGMAAAYGFDPARAQTPV
                     LQVGIIYQATADATQCDFGEVFTTDGRIAALDLVTLTDDKQFFAHYNPSVTMKSAFFD
                     AHHEIADVTAPVTAALTNDAIIEMNKQVDVEGRDPAVVARDWMVSKGFVTA"
     gene            complement(1994..2761)
                     /locus_tag="VGP27_21125"
     CDS             complement(1994..2761)
                     /locus_tag="VGP27_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853836.1"
                     /translation="MTNAVDTTAPKTTPAERIRLFIQPVGVLVVGAAVIFWAFNRNLT
                     ATQKENINVGNVSTLIWQHLLITFAVTAIVLAIGVPLGILVTRPGAKLLRPLFVGVAN
                     IGQAAPAIGLLVLLFLWTSRTGFWIGVLPIALYSLLPVLASTILGIDQVNPALIDAGR
                     GQGMAKSTMLLRVELPLAVPYILGGLRTSLVIAVGTATLSFLVNAGGLGILIDTGYKL
                     QDNVTLILGSVLAVCLALLVDWLGGLAEFFLNPKGLR"
     gene            complement(2758..3912)
                     /locus_tag="VGP27_21130"
     CDS             complement(2758..3912)
                     /locus_tag="VGP27_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853837.1"
                     /translation="MTEQSGARILLDHVSKVYPGSSEPAVDDTSLEIPAGEIVIFVGP
                     SGCGKTTMMRMINRLSEPTSGRILIGEKDALSIKPTELRRSIGYAIQQAGLFPHMTIR
                     QNVGLVPGLLRWDRKRIADRVEELLDLVGLEPGQYADRYPRQLSGGQQQRVGVARALA
                     ADPPVLLMDEPFGAVDPITRSTLQDELLRLQSELHKTIVFVTHDFGEAIKLGDRIAVL
                     GQQSKVLQYDTPQAILAGPADETVAGFVGSGASLRQLSLVRVKDVELRQHPAVHRGDS
                     VKTVQNALADSEFDWVVVLDDRDRPVSWVRESRLRHAASLADASESLDVVSTQSTLED
                     ALEAILAEQHASAVVTGPGSRYAGVVTLDTLINTITRLRSDAQSAGEDDE"
     gene            complement(3909..4556)
                     /locus_tag="VGP27_21135"
     CDS             complement(3909..4556)
                     /locus_tag="VGP27_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7853838.1"
                     /translation="MGALWDYLIAHHSQLMFDSYQHVSAVVQSVVIATLIGVAIGVLT
                     YRNPLAANLATTTSSVILTVPAFALLGLLIPVFGLGVAASVAALVLYSLLPIIRNTIV
                     GLNAVDPALIDAARGIGMSRLSRLGRVELRLVWPSILSAMRIATQMSMGVLAIAAYVK
                     GPGLGNLIFAGLARVGSPTAIPMALSGTLLIVILALLLDAMLVLIGRLTTSKGLQ"
     gene            4747..>5031
                     /locus_tag="VGP27_21140"
     CDS             4747..>5031
                     /locus_tag="VGP27_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7853839.1"
                     /translation="MPSSVRVVKLGAHIGARVDGIRLADGVDATAAAQINAALLEHKV
                     IFFRDQHDLSDEGQLAFARTLGTPTTAHPTVTSRGTRLLPIDSRYDKANSW"
BASE COUNT          918 a         1677 c         1600 g          836 t
ORIGIN      
        1 gtcaagcaca gaccctgcag aatcgggata tccagtgcgg caaggtgtgc cacgttccag
       61 gtgtcgtcgt tgccgcctgc agacacggcg gcaggaaccg ctccgcctgc ggccagcacg
      121 gtggtcacca tggcgtcggc cgatttcagc aagtccagca tgtcgctttc ggcggtacgc
      181 aacgaggcgc agtagacggg cagcgcgcgc ccaccggcgt cttcgatcgc agcgcaaagc
      241 gcttggacgt agccggtgtt gcccgcgagc tgttgagccc ggtagaagag cacggcgatc
      301 gtcgggccat caccattggt cgacgcgcgt tcgagcacac cccagttcgg gatgatcgcg
      361 ggcggcgcga acccgaagcc ggtcatcagc acggtgtcgc acagaaatgc gtgcagctgg
      421 cggaggttat ccatgccgcc ctgagcgaga tagatgtgcg cctgcaccgc cgcccctgcc
      481 ggaaccgtgg agtggctcat cagctcggcg tccggggcct gctcaccgct gaccaccacc
      541 gtcggcacgc cgctgccgat cacggtgtcg atgccgtcct gccaggatcg gtagccaccg
      601 agtatccgca cgacgacgag gtccgcgccg tcgatctgct cggtcagctc accgtccacg
      661 agccgcgacg ggttggccca ccgataccgc gcgccactcg agcgggcggc gatcaggtcc
      721 gtgtcagacg tcgacagcaa caggatggtg ggctcaggca cccaccattc ctatcgcagt
      781 gggcggtcag tcgccgaagc ggccgtgcca ggtctggggg tagggcaggc cggtcacggc
      841 ggcaaggacg agaagtacag cgccgaagac gatcaatgag aacatcgttg gtccttccgt
      901 acgttgttgc acacacgtgc aactgcgagc accgtacgac gacaccggcg attaatgatc
      961 gacatgtgcc tgacggaact cgcagaccag cggtgtgacg acggtcacat tcacgcagta
     1021 acgaagccct tggagaccat ccagtcgcgc gccaccacgg cagggtcacg gccctcgacg
     1081 tcgacctgtt tgttcatttc gatgatcgcg tcgttggtca gggcggccgt taccggcgcc
     1141 gtcacatccg cgatctcgtg gtgcgcgtcg aagaatgcgc tcttcatcgt gaccgacggg
     1201 ttgtagtggg cgaagaactg cttgtcgtcg gtgagcgtca cgagatccag tgcggcgatg
     1261 cggccgtcgg tggtgaagac ctcgccgaag tcacactggg tggcgtcggc cgtcgcctgg
     1321 tagatgatcc cgacctgcag caccggcgtc tgcgccctcg ccgggtcgaa gccgtaggcc
     1381 gccgccattc ctggatagcc gtcctgccgc gcccggaatt cggtgtccac acacgttttc
     1441 gcggccgcgg gattcttctt cacgagatcg gcgtactgcg acagggtccg aacgccggtg
     1501 cgctccacca ctgccttgct cgccgcaagt gcataggtgt cgtccatcgg gcccggcggc
     1561 agccacacca tgtcattgcg cttcaggtct tccgcccgca ccgcctcgta ttgctcgcgg
     1621 ctgtcgggta tcggcttctc gtggccgagg tagttgatcc aggcgttgcc ggtgaattcg
     1681 taggcgacgt cgacctgccc tcgcagttgc gcttccctcg tgcttcgcga tccgacgatg
     1741 ccggtcagat cgcgcacctc ggcgcccgct gcggccaagg tgtactcgag gatgtagccc
     1801 atgatcacct gctcggtgta ctccttggac cccaccgtga tcttgacacc ggccagcgca
     1861 ggattcggct tgatcgaacc gggagcgacc atgaacggca cggccccgcc cgaaccgaga
     1921 ccgcacgccg tcaccaccgc cgcacacacc gccgcgacaa gcgatgtcca catcccgcgc
     1981 ttcaacgcgc ccatcagcga agtcccttgg gattgaggaa gaactcggcc agtccgccca
     2041 gccagtcgac cagcagcgcc aagcagacgg cgagcacact gcccaggatc agcgtgacat
     2101 tgtcttgcag tttgtagcct gtgtcgatga ggattccgag accgcccgcg ttgacgagga
     2161 acgacagcgt cgcggtgccg acggcgatca cgagcgaggt ccgcagcccg cccaggatgt
     2221 acggaaccgc cagtggcagc tcaactctga gcaacattgt gctcttggcc atcccctgac
     2281 cacggcctgc atcgatcagc gctgggttca cctggtcgat gccgaggatg gtgctggcca
     2341 gtacgggcag cagcgagtac agagcgatcg gcaacacccc gatccagaac cccgtcctgg
     2401 aagtccacag aaacagcagc accagcagac cgatggcagg cgcagcctgg ccgatgttcg
     2461 ccacgccgac gaacaagggc cgcaacagct ttgcacctgg ccgcgtcacc aggattccga
     2521 ggggaacacc gatcgccagc acgattgcgg tgaccgcgaa ggtgatgagc agatgctgcc
     2581 agatcaacgt cgagacgttg ccgacgttga tgttctcctt ctgtgtcgcc gtcaggttcc
     2641 ggttgaaagc ccaaaagatc accgccgccc cgacgacaag gacacccacc ggctgaatga
     2701 acagccggat acgctcagcg ggggtcgttt tcggcgcggt cgtgtcgacg gcgttcgtca
     2761 ctcgtcgtcc tcgccggccg attgggcgtc ggaacgcagc cgggtgatgg tgttgatcaa
     2821 ggtgtccagc gtcaccacac ccgcgtacct gctgcccggg ccggtgacga ctgccgacgc
     2881 gtgctgttcg gcgagaatcg cctccaacgc gtcttcgagc gtcgactggg tgctgaccac
     2941 atcaagcgat tcgctcgcgt cggcaaggga cgcggcatgg cgaagccggc tctcgcgcac
     3001 ccagctcacc ggccggtcgc gatcgtcgag cacgacgacc cagtcgaatt cgctgtcggc
     3061 caaagcgttc tgcaccgttt tgacgctgtc gccgcgatgg acggcgggat gctgccgcag
     3121 ctcgacatct ttgacccgca ccaggctgag ctgccgcagc gacgcaccgg aaccaacgaa
     3181 gccggccacc gtctcgtcag cggggccggc caggatcgcc tgcggcgtgt cgtactgcaa
     3241 caccttcgac tgctggccca gaaccgcgat acggtcgccc agtttgatcg cctcgccgaa
     3301 gtcgtgcgtg acgaacacga tggtcttgtg cagctctgac tgcagccgca gcagttcgtc
     3361 ctgaagggtg ctgcgcgtga tcgggtcgac ggcgccgaac ggctcgtcca tcagcaacac
     3421 cggcggatcg gccgccagag cgcgcgctac cccgacccgc tgctgctggc cgcccgacag
     3481 ctgacgcgga taccggtcgg cgtactggcc gggctcgagg ccgacgaggt cgagaagctc
     3541 ctcgacgcgg tcggcgatcc gcttgcggtc ccaccgcaac aggccgggga ccaggccgac
     3601 gttctgccga atagtcatgt gcgggaacaa accggcctgc tgaatcgcgt agccgatcga
     3661 acgtcgcaac tccgtcggct tgatcgacag cgcatccttc tcaccgatca ggatgcggcc
     3721 cgacgtcggc tcgctgagcc ggttgatcat ccgcatcatc gtggtcttcc cacaccccga
     3781 cggcccgacg aagatcacga tttcgcctgc cgggatttcg agcgaggtgt cgtcaacggc
     3841 gggctccgag gaccccgggt acaccttcga cacgtggtcc agcaggatcc gcgccccgga
     3901 ttgttctgtc attgaaggcc tttcgacgtc gtcagccgtc cgatcagaac caacatcgca
     3961 tccagcagaa gtgccaggat gacgatgagc agggtcccgg acagtgccat cgggatcgcg
     4021 gtggggctac cgaccctggc cagcccggcg aagatgaggt tgcccaatcc ggggcctttc
     4081 acgtaggccg cgatcgccag cacacccatc gacatctgcg tggcgatccg catcgcggac
     4141 aggatggacg gccacaccaa acgcagctcg actcggccaa gcctcgacag ccggctcatg
     4201 ccgatgccac gggccgcgtc gatgagcgcc ggatcgacgg cgttcaggcc gacgatcgtg
     4261 ttgcggatga tcggcagcag cgagtacagc accagcgcgg cgacgctggc cgcaacgccg
     4321 aggccgaaga cgggaatgag caggccgagc agtgcgaacg caggcacggt gaggattacg
     4381 ctcgacgtcg tggtggcgag gttggccgcc agcgggttgc ggtaggtcag cacaccgatc
     4441 gccacgccga tgagggtggc gatcaccacg ctctgcacga cggcgctgac gtgctggtac
     4501 gagtcgaaca tcagctgcga atgatgcgca atgaggtagt cccacaaagc gcccacattc
     4561 aactccccgc tacgcggcaa cctgtaagtg ccattgcgcc cactggccgg tacccgatcc
     4621 tggcaaatgc caaaccaaga tcaaaagatc tcgcacagtt gcgttctcgc tgagttgaaa
     4681 tcttggcggc gagccgacgg gccgccattc tgctacgggg tgttctgcgt ctgtggaggt
     4741 gaactcgtgc cctcgtccgt gcgtgtcgtc aagctcgggg ctcatatcgg tgcccgcgtc
     4801 gacggcattc gccttgccga tggtgtcgac gccaccgccg ccgctcagat caatgcggcg
     4861 ctgctcgagc acaaggtgat cttcttccgg gaccagcatg acctgagcga cgagggccag
     4921 ttggcgttcg cgcgcacgct gggaacgccg accaccgccc acccgacggt gacgtcgcgc
     4981 ggaacccgcc tgctgcccat cgactcgcgc tacgacaagg ccaacagctg g
//
LOCUS       DASYLX010000905         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3564895,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000905 DASYLX010000000
VERSION     DASYLX010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3564895"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VGP27_21145"
     CDS             <1..896
                     /locus_tag="VGP27_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7853840.1"
                     /translation="GDDVSIYTRSLDDVTARLPEVVEATLALPVKDLIADGEAIALRP
                     DGRPHRFQVTASRFGRGGGRGTKDRTVDISAARAAQPLSVFFFDVLHLDGADLVDEPT
                     RNRLAALDAIVPPRQRVDRLLTSDSEAAQRFLDVTLAAGHEGVMAKSPTAPYEAGRRG
                     AGWLKVKPVHTLDLVVLAVEWGSGRRTGKLSNIHLGARDPATGDFVMLGKTFKGMTDA
                     MLDWQTARFTELADGPTDGWVVKVRPEQVVEIAFDGVQTSARYPGGMALRFARVLRYR
                     DDKGPADADTVDTVRAFYERE"
     gene            999..>3285
                     /locus_tag="VGP27_21150"
     CDS             999..>3285
                     /locus_tag="VGP27_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEV7853841.1"
                     /translation="MASPTTESTERFEETDGDITYICTEKDLPPVAIIDRSPITTRHK
                     IIFGIIAILGAIAWAVIAFFRGETVNAVWFVIAAICTYVIGFRFYARLIEMKIVRPRD
                     DNATPAELFENATDYMPTDRRVLFGHHFAAIAGAGPLVGPVLAMQMGYLPGTIWIIIG
                     AVFAGCVQDYLVLAISTRRRGRSLGQMARDELGAIGGAAAIVGVLVIMVILLAVLALV
                     VVGALAESPWGVFSIAMTIPIAIFMGLYLRFLRPGRVSEVSLIGVGLLLLAVVSGGWV
                     AETAWGTDWFTLSKVSLSWCIIIYGFAASVLPVWLLLAPRDYLSTFMKVGTIALLALG
                     ILLARPIMEAPAISQFARTGTGPVFAGSLFPFLFITIACGALSGFHALIASGTTPKLL
                     EKESQMRLIGYGGMLTESFVGIMALITAAILNQHLYFAINAPSAQTGSTAATAAQYVN
                     GLGLSGAPITPEQLTQAAEDVGEDSIISRTGGAPTLAIGMSEVLHQVFGGTALKAFWY
                     HFAIMFEALFILTTVDAGTRVARFMLSDGLSNFGGPLRKLKDPSWRVGAWFCSIVVVA
                     AWGGTLLMGVTDPLGGINTLFPLFGIANQLLAAIALTVVTVVVIKRGLLKWAWIPALP
                     LFWDLIVTMTASWQKIFSADPKLGYWKQHSSCQAAKDAGKLCLTAKTPADVDKIVRNT
                     FIQGSLSILFAALVIVVFVAGVIVALRTIRGQGKPLTEDEPVPSRLFAPSGLVPTKAE
                     REVQKQWDAQPKSHTKSVGTGA"
BASE COUNT          525 a         1083 c         1060 g          617 t
ORIGIN      
        1 ggggcgacga cgtgtcgatc tacacccgca gcctcgacga cgtcaccgcc cgactgcccg
       61 aggtggtcga ggcaacgctc gcccttccgg tgaaggatct gatcgccgac ggagaggcga
      121 tcgccctgcg ccccgacgga cggccgcacc ggttccaggt caccgcgtca cgcttcggtc
      181 gtggcggggg ccgcggcacg aaggaccgca ccgtcgacat ctcggccgcc cgtgccgcgc
      241 agcccttgtc ggtgttcttc ttcgacgtcc tgcacctcga cggcgccgac cttgtcgacg
      301 agccgacgcg caatcggctc gcggcgctcg acgcgatcgt ccctccccgc cagcgcgtcg
      361 accgactgct gacctccgat tccgaagcgg cgcaacgatt tttggatgtg acactggctg
      421 ccgggcacga gggcgtgatg gcgaagtcgc caactgcgcc gtacgaggcg ggtcgccgcg
      481 gcgcaggctg gttgaaggtg aagccggtac acaccctcga cctggtcgtg ctggccgtcg
      541 agtggggatc gggccggcgc accggcaagc tgtcgaacat ccatctcggt gcgcgcgatc
      601 cggcgactgg cgatttcgtc atgttgggta agacgttcaa aggcatgacc gacgcaatgc
      661 tggactggca aaccgcccgg ttcaccgaat tggccgacgg cccgaccgac ggctgggtcg
      721 tcaaggtccg ccccgaacag gtggtggaga tcgccttcga cggcgtgcag acgtcggccc
      781 gctatcccgg tggtatggcg ctgcggttcg cgcgggtgct gcgctaccgg gacgacaaag
      841 gccccgccga cgctgacacc gtcgatacgg tgcgagcgtt ctacgagcgg gaatagcaag
      901 taattcgggc atcttcggca atcctcttca ggcccggcac cgcctttgaa aggatcacag
      961 tcggcaccca ggctgcactg cgatcgaggg agggcactgt ggcttcaccc accaccgaat
     1021 cgaccgaacg cttcgaggaa accgacggcg acatcaccta catctgcacc gagaaggatc
     1081 tcccgccggt ggcgatcatc gatcgatcgc cgatcaccac ccgccacaag atcatcttcg
     1141 gcatcatcgc gattctcggc gcgatcgcgt gggcggtgat cgccttcttc cgcggggaga
     1201 ccgtcaacgc cgtctggttc gtgatcgctg cgatatgcac ctatgtgatc gggttccgct
     1261 tctacgcccg gctcatcgag atgaagatcg tgcgtccgcg tgacgacaat gcgacgcccg
     1321 cagagctttt cgagaacgcc accgactaca tgccgacgga tcgccgggtg ctgttcggtc
     1381 accatttcgc ggcgatcgcg ggtgccggcc cgctggtcgg ccccgtgttg gccatgcaga
     1441 tgggatatct gccgggaacc atctggatca tcatcggcgc ggttttcgcc ggctgcgtcc
     1501 aggactacct ggtgttggcg atctcgacca ggcgacgagg gcggtccctc ggccagatgg
     1561 ctcgcgacga actcggggcg atcggcggcg ctgcggccat cgtcggcgtg cttgtcatca
     1621 tggtgatcct gctcgcggtg ctggccctgg tggtggtcgg cgccctcgcc gagagtccgt
     1681 ggggcgtctt ctcaatcgcg atgaccatcc ccatcgccat cttcatgggc ctttacctga
     1741 ggttcctgcg gcccggtcgg gtgtccgagg tctcgcttat cggtgtcggc ctgctgttgc
     1801 tcgccgtcgt ttcgggtggc tgggtggccg aaacggcctg gggcaccgac tggttcacgc
     1861 tgtcgaaagt gtctctttcc tggtgcatca tcatctacgg cttcgcggcg tcggtgctgc
     1921 cggtgtggtt gctgctggca ccgcgcgatt acctgtcaac cttcatgaag gtgggcacca
     1981 tcgccctgct ggcgctcggc attctgctcg cccgccccat catggaggcg cccgcgatct
     2041 cgcagttcgc ccgcaccggc accggacccg tgttcgcggg ctcgttgttc cccttcctgt
     2101 tcatcacgat cgcgtgcggc gcgctgtcgg gtttccacgc gttgatcgcg tcgggcacca
     2161 ccccgaagct gctcgaaaag gaaagccaaa tgcgactgat cggctacggc ggcatgctca
     2221 ccgagtcgtt cgtcggcatc atggcgctca tcaccgccgc gatcctgaac cagcacctgt
     2281 acttcgccat caacgcaccg tcggcccaga ccggctcgac ggctgccacc gcagcccaat
     2341 acgtcaacgg cctcggattg tcgggcgcgc cgatcacacc cgaacagctc acccaggccg
     2401 ctgaggatgt cggcgaggac tcgatcattt cgcgcaccgg cggcgcgccg accctggcga
     2461 tcggcatgtc cgaggtcctg caccaggtgt tcggcgggac agcgttgaag gcgttctggt
     2521 accacttcgc gatcatgttc gaggcgctgt tcatcctcac cacggtcgac gcgggaacga
     2581 gggtggcccg gttcatgctc tccgacgggc tgagcaactt cggcggtccg cttcgcaagc
     2641 tgaaggaccc gagttggcgc gtcggcgcgt ggttctgcag catcgtcgtt gtcgccgcgt
     2701 ggggcggcac cctgctgatg ggcgtcaccg acccactcgg cggcatcaac acgctgttcc
     2761 cgctgttcgg catcgccaac caactgctgg cggccattgc gctgacggtg gtcaccgtgg
     2821 tcgtcatcaa gcgcgggttg ctgaaatggg cctggatacc ggcacttccg ctgttctggg
     2881 acctgatcgt gacgatgacg gcatcgtggc agaagatctt ctcggcggat cccaagctcg
     2941 gctactggaa gcaacacagc agttgccagg ccgcaaagga cgccgggaag ctatgcctga
     3001 cggcgaagac tcccgccgac gtcgacaaga tcgtgcgcaa caccttcatt cagggatcgc
     3061 tgtcgattct gtttgcggcg ctggtgatcg tcgtgttcgt tgcaggcgtg atcgtggcgc
     3121 ttcggacgat acgcgggcaa ggcaaaccgt tgaccgagga cgaacccgtg ccgtcccgtt
     3181 tgttcgctcc gtccgggctc gttcccacca aggccgaacg ggaggtgcag aagcaatggg
     3241 acgcgcagcc gaaatcgcac accaaatcag ttggtactgg ggcac
//
LOCUS       DASYLX010000906         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2359161,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000906 DASYLX010000000
VERSION     DASYLX010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359161"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VGP27_21155"
     CDS             <1..117
                     /locus_tag="VGP27_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEV7853842.1"
                     /translation="PGNVFGAVVEGLDEMAEACQDLWMGGARGETLSFQRRD"
     gene            complement(287..1186)
                     /locus_tag="VGP27_21160"
     CDS             complement(287..1186)
                     /locus_tag="VGP27_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853843.1"
                     /translation="MARTDNDSWDLASSVGATATMVAAQRVLADREGLIDDPYAEPLV
                     RAVGLDVFTKALDGEITLEDIDPRFTIQRAAQGMAVRTRHFDRLFSDAAAAGVRQAVI
                     LAAGLDARAYRLPWPDGTTVFEVDQPEVIDFKSTTLADLGAQPTADRRTVAIDLRDDW
                     PKALRDNGFDPSQPTAWIAEGLLIYLPSEAQDLLFDRIDELSAPGSRVATEHIPDVSM
                     FSDERSQLISDRLKQYGHDFEMGDLIYHEERNDVIEYLEGHGWDVAARKMADAYADNG
                     FEFPEDGAIGFFTGMSYLSAIKR"
     gene            complement(1195..2088)
                     /locus_tag="VGP27_21165"
     CDS             complement(1195..2088)
                     /locus_tag="VGP27_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853844.1"
                     /translation="MARTDNDTWDLASSVGATATAVAASRAVASQGPDPLLDDPFAEP
                     LVRAVGVDNFNRIVDGEAFNDDPVMNRKAMSEQIAVRTRFFDDFFLAASEAGIRQAVI
                     LAAGLDTRGYRLPWPAGTVVFEVDQPQVIEFKTTTLDGLGAVPTATRKTVAIDLRDDW
                     PAALREAGFDPTRPTAWIAEGLLVYLPPDAQDRLLDNVTALSAPGSRLATEHMDMTSM
                     PDDWTEKLDERSRRAGSDIKLSELFYSGDRNTAREYLSSHRWQVSTISTAEAFAANGF
                     EVPDDELIRFIGDSGYLTATL"
     gene            complement(2096..>2646)
                     /locus_tag="VGP27_21170"
     CDS             complement(2096..>2646)
                     /locus_tag="VGP27_21170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7853845.1"
                     /translation="AGTVVYEIDQPKVIEFKNATMSAIGAAPTAERRTVSIDLRDDWP
                     AALRRNGFDETQPTAWIAEGLLVYLPPEAQDRLFDDITALSAPGSRLATEYHPNPGAS
                     IGERAQAIGDRWKKHGFDINLADLFYPGERNPVVDYLAGQGWQVTARARPEMFETYGR
                     KFPDGDELAPMRDSLAVTATRQ"
BASE COUNT          436 a          854 c          866 g          490 t
ORIGIN      
        1 cctggcaatg tcttcggcgc ggtggtcgag ggactcgacg agatggctga ggcgtgccag
       61 gacctgtgga tgggcggcgc acgcggcgag acactgagct ttcagcgccg cgactgacgg
      121 cgggggccac gtgctgcttc ccggctatga cctgcttacc cgcccacccg cccgttcgtc
      181 gccgcgcgag cgtgcgcact tgcacgaaat gtgacggcga gtcgcgtgca gacacgcacg
      241 ctcgcggtgc gaggttacag ccgcttttcg ggggcggggg agatccttag cgcttgattg
      301 cgctcaggta gctcattccg gtgaagaagc cgatggctcc gtcctcggga aactcgaaac
      361 cgttgtcggc atatgcatcc gccatctttc gcgccgccac atcccagccg tgaccctcga
      421 ggtactcgat gacgtcattg cgttcctcgt gatagatgag gtcgcccatc tcgaaatcgt
      481 ggccgtactg cttgagccga tccgagatca gctgcgaccg ctcgtcagag aacatgctga
      541 catcggggat gtgctcggtg gccacccgac tgcccggcgc gctgagctcg tcgatcctgt
      601 cgaacaacag gtcctgcgcc tccgacggaa gatagatgag caggccctcg gcaatccatg
      661 cggtgggctg cgacgggtcg aagccgttat cccgcaatgc tttgggccag tcgtcgcgaa
      721 ggtcgatcgc gacggtgcgt cggtcggccg ttggctgcgc gccgaggtcg gcgagcgtcg
      781 tggacttgaa gtcgatcacc tcgggttgat cgacctcgaa cacggtcgtt ccgtccggcc
      841 acggcaatcg ataggcccga gcgtccaagc ccgccgccag aatcaccgcc tggcgtacgc
      901 ccgcagccgc cgcatcgctg aacaacctgt cgaaatggcg ggtgcggacc gccatgccct
      961 gtgcggcgcg ctgaatggtg aatcgtggat cgatgtcctc cagcgtgatc tcaccgtcaa
     1021 gcgccttagt gaagacgtcc aggccgaccg cacgcactaa cggctcggcg tacggatcgt
     1081 cgatcaaccc ctcgcgatcg gccagcacgc gctgcgcggc gaccatcgtg gcggtggcgc
     1141 cgacgctgga cgcgagatcc cacgagtcgt tgtcggtgcg ggccatcatg ctccttacag
     1201 ggttgccgtg aggtagccgg agtcgccgat gaacctgatg agctcatcgt ccggcacctc
     1261 gaaaccgttg gcggcgaacg cctccgcggt ggagatagta gacacttgcc agcggtgcga
     1321 ggacaggtat tcacgcgccg tattgcggtc gcctgagtag aacaactccg acaacttgat
     1381 gtccgagccg gcccgacgcg atcgttcgtc cagcttttct gtccagtcgt ccggcatcga
     1441 cgtcatgtcc atgtgttcgg tggccaaccg actgccagga gcggacagcg cggtgacgtt
     1501 gtccaggaga cgatcctgcg catccggtgg cagatagacc aaaaggccct cggcgatcca
     1561 cgcggtgggc cgagtcgggt cgaagcccgc ctcgcgtagt gcggccggcc aatcgtcgcg
     1621 caggtcgatg gcgaccgtct tgcgcgtggc tgtcggaacc gcaccgaggc cgtcgagcgt
     1681 cgttgtcttg aactcgatga cttgcggttg atcgacttcg aagaccaccg tgcccgccgg
     1741 ccatggaagc cggtagccgc gggtgtccag tcctgccgcc agaatcaccg cctgccgtat
     1801 cccggcttcg gaagcggcaa ggaagaagtc gtcgaaaaac cttgtcctga ccgcgatctg
     1861 ctcactcatc gccttgcgat tcatcaccgg gtcgtcgttg aacgcctcgc cgtcgacgat
     1921 cctattgaaa ttgtccacgc cgaccgcgcg gaccagcggc tcggcgaacg gatcgtcgag
     1981 tagcgggtcg ggcccctgag aggccaccgc tcgagaggcc gccacagcgg tggcggtagc
     2041 gccaacgctc gaggccaggt cccaggtgtc gttgtctgtt cgtgccatcg aatgcctact
     2101 gtcgtgtcgc ggtgacggcc agcgaatctc gcatcggtgc gagctcgtcg ccgtcgggga
     2161 atttacggcc gtacgtctcg aacatctcgg gccgtgcgcg cgcagtcacc tgccaccctt
     2221 gtccggccag gtagtcgacg accggattgc gctcgcccgg atagaagagg tcggcgaggt
     2281 tgatgtcgaa cccgtgtttt ttccaccggt cgccgatcgc ctgtgcgcgc tcaccgatgc
     2341 tcgcccccgg attggggtga tactcggtgg ccagcctgct gcccggcgcg ctgagcgcgg
     2401 tgatgtcgtc gaacagccgg tcctgagcct ccggaggcag gtacaccaga agcccttcag
     2461 cgatccatgc cgttggctga gtctcgtcga atccgttgcg ccgcaacgcg gcaggccagt
     2521 cgtcgcgtag atcgatgctc acggtgcggc gctccgcggt gggcgccgca ccgatcgcgg
     2581 acatcgtcgc gttcttgaac tcgatcacct tcggctggtc gatctcgtag acgacggtgc
     2641 ctgcgg
//
LOCUS       DASYLX010000907         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_383078,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000907 DASYLX010000000
VERSION     DASYLX010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383078"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGP27_21175"
     CDS             complement(<1..486)
                     /locus_tag="VGP27_21175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853846.1"
                     /translation="MARTDNDTWDLASGVGATATMVAAARAIATKADNPLIDDRFADP
                     LVRAVGVDFFTRWAAGELDAADLDDNESPWRLEQMPDAMAARTRYFDSFFLDATRAGI
                     RQAVILASGLDARAYRLAWPADMTVFEIDQPRVIEFKTTALAGLRAAPTADLRTVSID
                     LR"
     gene            complement(522..1142)
                     /locus_tag="VGP27_21180"
     CDS             complement(522..1142)
                     /locus_tag="VGP27_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7853847.1"
                     /translation="MTLDLTPDQLLSTTRTVRKRLDLSRPVEQELLQECFDLALQAPT
                     GGNQQGWHFVVVTDADQKKALAELYRKAKNETEPPSAPPGQEAVMNSAAYLAEHLQEV
                     PVLVIPCIEGRYENAPHLWQAVAWASILPATWSFMLAARARGLGTAWTSLHLAHERDA
                     AAVLRIPYEQVTQAALIPVAHTIGTEFRPGPRSPQAEHIHWDSFTS"
     gene            1286..1933
                     /locus_tag="VGP27_21185"
     CDS             1286..1933
                     /locus_tag="VGP27_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7853848.1"
                     /translation="MSHLLHIDSSVRGDDSVSRRLTARAADRWRGAHPDGTVTYRDLG
                     TSPIPHLDAATGLARMVQAEQRTPAQDASFALSAELIDEIKQADTVLLGLPLYNFGPP
                     STVKAWVDHLIATGLSIDAETHTGLLGDTDFIVLASRGGGYGPGTPREGWDHAQTWLT
                     HALSVTGLQPQFITAELTTADANPTMAHLKPLAAESLNTALAAIDQLWTADEDAA"
     gene            complement(2081..2644)
                     /locus_tag="VGP27_21190"
     CDS             complement(2081..2644)
                     /locus_tag="VGP27_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853849.1"
                     /translation="MQRGARSQQVVEKVRAATMAELARVGFGGLTIEGVAKAANVNRT
                     TIYRRWPSKSALLLAVLEPGLERFDHDPDTGSLGGDLLALMLMIGQTAALPEGQAVHR
                     AVGSTSEELKELVEAVNDRGLGAFRRAFDRARARGEVEPTDDTEVMAHLAFFGVVLWG
                     ETRERAPTEEECRRILRVVLAPLAGSS"
BASE COUNT          508 a          975 c          897 g          497 t
ORIGIN      
        1 tcgcaggtcg atggatacgg tgcgcaggtc agccgtcggg gcggcgcgca gacccgccag
       61 tgcggtggtc ttgaactcga tgacgcgtgg ctggtcgatc tcgaacaccg tcatatcggc
      121 gggccacgcc agccggtagg ctcgcgcgtc gagaccggat gccagaatca ccgcctggcg
      181 gatgccggcc cgggtcgcgt cgaggaagaa cgagtcgaaa tagcgagtgc gggcggccat
      241 cgcgtcgggc atctgctcca gccgccacgg cgactcgtta tcgtcgaggt cggcggcgtc
      301 gagttcaccg gcggcccacc gggtgaagaa atcgactccg accgcacgga ccaggggatc
      361 ggcgaaccgg tcgtcgatca acggattgtc ggccttggtg gctatcgccc gggcggcggc
      421 gaccatcgtc gccgttgcgc ccacgccgct ggccaagtcc caggtgtcgt tgtctgtgcg
      481 cgccatgcgc tgtccttccc aatctcagcc aaataaaata cttagctagt aaaactatcc
      541 cagtgaatgt gttcggcttg gggactcctg ggtccgggcc tgaactcggt accgatcgtg
      601 tgtgcaaccg gtatcagcgc cgcctgggtc acctgctcat agggaatcct gagcactgcg
      661 gcggcgtcac gttcgtgggc caggtgcaat gacgtccacg ccgttcccaa gcccctggcc
      721 cgggcggcaa gcatgaaact ccaggtcgcg ggcaggatcg atgcccacgc tacggcttgc
      781 cacaggtgcg gagcgttttc gtagcggcct tcgatgcacg ggataaccag gaccggcacc
      841 tcctggaggt gctcagccag gtaggcggcg gagttcatca cggcctcctg cccgggtggg
      901 gccgagggtg gctcggtctc gtttttggct ttccggtaca gctcggcaag agccttcttt
      961 tggtcggcgt cggtgacgac gacgaagtgc cagccctgct ggttgccgcc ggtcggagct
     1021 tgcaaggcca ggtcgaagca ctcctgcaac agctcctgct cgacagggcg cgacaaatcc
     1081 aggcgtttgc gcacggtgcg cgtggtcgac aacaactgat ccggcgttag gtcaagcgtc
     1141 atgtgtgcac gctcctcgtc gtgtggtgcg tcgaactgtc cataagcccc cacgctagca
     1201 caaatggcaa cgctatgttg ccattcggcg atttctgcgc tagcttcacg ttggacggtc
     1261 aaccaagatc tatggaggtt tagcgatgtc ccatctgctg cacatcgatt cctcggtccg
     1321 aggcgacgac tcggtgagtc gtcgcctgac cgctcgggcc gccgatcggt ggcgaggcgc
     1381 ccatcccgac ggaacggtga cctaccgtga tctcggcacc agcccgatcc cgcacttgga
     1441 cgctgcgacc ggactggcgc gaatggtgca agccgagcag cgcaccccgg cacaagacgc
     1501 gtccttcgcg ctcagcgccg aactgatcga cgagatcaaa caggccgaca cggtcctgct
     1561 cggcctgccg ctctacaact tcggtccgcc cagcaccgtc aaggcctggg tcgaccacct
     1621 catcgccacc ggcctgtcca tcgacgccga gacccatacc gggctgctcg gcgacaccga
     1681 cttcatcgtg ctcgccagcc gcggcggcgg ttacggtccg ggcacgccgc gcgaaggctg
     1741 ggatcatgcc cagacctggc tgacacacgc cctgtcggtg accggactgc agccgcagtt
     1801 catcaccgcc gaactcacga cggccgatgc caacccgacg atggcccatc tcaaaccgct
     1861 tgccgctgaa agcctcaaca cagcgctcgc cgccatcgac cagctctgga cggccgacga
     1921 agacgccgcc tagtgcgcga agccagccca acaaaccggc aaccgaaaag gttgatagac
     1981 gtcacgatct ccaaagaagc ttcatagttg ccaccggcgc ggctcccaat cctgtgtgcc
     2041 gacagccact gattcacttg gctgagcgcg cagatggtgc tcaactactg ccggccagcg
     2101 gcgcgagcac gacgcgcaat atgcggcggc actcctcctc ggtcggcgcg cgctcgcggg
     2161 tctcccccca cagcacgacg ccgaagaacg ccaggtgcgc catcacttcg gtgtcatcgg
     2221 tcggttccac ctcgccccgg gcgcgggcgc gatcgaaggc gcgacgaaaa gcgccgagcc
     2281 cacggtcatt gacggcttcg acgagctcct tgagttcctc cgacgtcgac ccgaccgcgc
     2341 ggtggaccgc ctgcccctcg ggcaaggctg cggtctgccc gatcatcagc atcagggcca
     2401 gcagatcccc gcccagtgaa ccggtatcgg gatcgtggtc gaagcgctca agccccggct
     2461 ccaggacggc caacagcagt gcgctcttgg acggccaccg ccgatagatc gtggtgcgat
     2521 tgacattggc ggctttggca actccctcga tggtcagccc cccgaacccc acccgtgcca
     2581 gttcggccat ggtggccgcg cgcaccttct ccaccacctg ctggcttcga gcaccgcgct
     2641 gcacgcccct cgttcgccgc gtcgaaatgt ccatagccac caccctagcg ttaatggcat
     2701 cagattgttg ccatttggga tatctgcgct ggcttcctct cggacagtcg gccagagaaa
     2761 ggactggggt cgttgacgga gcacgcggcg aactgggcga ctgtcggctg gtcggcaggt
     2821 ttagctcaag accgccaggc ccgcggtgtc tcagatgatc tgcgagagct atcccgt
//
LOCUS       DASYLX010000908         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2468088,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000908 DASYLX010000000
VERSION     DASYLX010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468088"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VGP27_21195"
     CDS             <1..123
                     /locus_tag="VGP27_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853850.1"
                     /translation="VVLCRSIRNAEPLRQVACQMEFLIKSREFVRRNGLPTGDR"
     gene            complement(120..641)
                     /locus_tag="VGP27_21200"
     CDS             complement(120..641)
                     /locus_tag="VGP27_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7853851.1"
                     /translation="MATIYYTASSIDGYIVDDRDSLEWLISRDIDVDGPFGYRAFGDS
                     VGALVMGATTYEWIVANDPDGWQYKQPTWVMTHRPNIIRDGDPVRAFQGDVTELQPLL
                     LQAAGYQDIWVVGGGDVAAQFARAGLIDEMIVSYAPCTLGSGGRLMPLHTEWTLVESG
                     TNGDFICARWRKA"
     gene            complement(720..1055)
                     /locus_tag="VGP27_21205"
     CDS             complement(720..1055)
                     /locus_tag="VGP27_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853852.1"
                     /translation="MAHSDELPIPDYDQLSLPDLRHRVRSLEEGPLRRLFDHETEHGN
                     RIPVLEILHARLKELHDGAEPSGGDQSNAPGVTPTPAGSTVQEATAAEPGTPLRHGVA
                     SQTPSRGKP"
     gene            complement(1093..1872)
                     /locus_tag="VGP27_21210"
     CDS             complement(1093..1872)
                     /locus_tag="VGP27_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7853853.1"
                     /translation="MSEEANGESPVLVEQRDRILIITINRPKAKNAVNAAVSRGLADA
                     MDRLDGEDGLSVGILTGAGGSFCAGMDLKAFARGENVVVEGRGMGFTERPPAKPLIAA
                     VEGYALAGGTELALATDLIVASRDSAFGIPEVKRGLVAGGGGLLRLPQRIPYAVAMEL
                     ALTGENLPAERAHELGLVNLLAEPGSALEAAIGLAERISSNGPLAVAATKRIIVEARG
                     WSPDEMWTKQNKILAPVFTSNDAREGAVAFAEKRPPKWTGS"
     gene            1963..2772
                     /locus_tag="VGP27_21215"
     CDS             1963..2772
                     /locus_tag="VGP27_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7853854.1"
                     /translation="MATTDIEPSELTKWDPGLTARVMGLTRPFLKRYFRSEVRGLDNV
                     PAGGALLVSNHSGGMLPMDVPILSAHYYEQHGYDRPLYTLSHDMLMMGPTGDFFKKIG
                     YISANHENADEALRSGGLVIVFPGGDYDVYRPTLSENKIDFGGRTGYIKAALNAGVPI
                     VPTVGIGGQETQIFLTRGTWLAKRLGPIARLARAKIVPISFGFPFGLSLVVPPNIPLP
                     TKIVMEVLPPIDIVAEFGENPDIDEVDAHVRHVMQRALDELAAERRLPILG"
     gene            2778..>3775
                     /locus_tag="VGP27_21220"
     CDS             2778..>3775
                     /locus_tag="VGP27_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7853855.1"
                     /translation="MALLDRLNDTLGLVATMRRAGMIAPLRPDKYVKIAAAMRRENVS
                     AVSGFASAAQRCPDRSGLVDELGTLTWREIDERCDAFAAGLQALPGGQPRVIGVMCRN
                     HRGFIEAVVAANRIGTDLLLLNTSFAGPALAEVVAREGADAVVYDEEFTEIVGRALAD
                     APDATRIVAWTDDPSALGDTPTVEKMIAAHAGREPKRAAQKPRTILLTSGTTGTPKGA
                     KLTGGDPSALKAILDRTPWHTEEAIVIAAPMFHAWGFSQMIFAATMACTIVTRRKFDP
                     EATLELVDRHRATGLAVVPVMFDRIMELPDGVRSRYSGRSLRFAAASGSRMRPDVVT"
BASE COUNT          582 a         1333 c         1235 g          625 t
ORIGIN      
        1 gtggtgttgt gccgctccat ccgcaacgcc gagccattgc gccaggtcgc atgccagatg
       61 gaattcctca tcaagtcacg agaattcgtc cgtcgtaacg gactgccgac cggcgaccgc
      121 taggcctttc gccacctggc gcagatgaag tcgccgttcg tgccggactc caccaaggtc
      181 cattccgtgt gtaggggcat caatcgcccg ccgctgccca gtgtgcacgg cgcgtagctg
      241 acgatcatct cgtcgatcag gccggcgcgc gcgaactgcg cggccacatc gccgccgccg
      301 accacccaga tgtcctgata gcccgctgcc tgcaacagca gtggctgcag ttctgtcacg
      361 tctccctgga aggcccgcac cggatcaccg tcccgaatga tattgggccg gtgggtcatt
      421 acccatgtgg gctgcttgta ctgccagcca tcggggtcat tggccacgat ccactcgtat
      481 gtcgtggcac ccatcaccag cgcgccgacg gaatcgccga acgcgcggta cccgaacgga
      541 ccgtcgacat cgatgtcgcg ggatatcaac cactccaagc tgtctcggtc gtcgacgatg
      601 tacccgtcga tgctcgacgc cgtgtagtag atggtcgcca cgtcagttgc ctctcatcgg
      661 ttcgagcggc atgtgcgcgg ccaccctcgg ccatgtcacc agatgcaaca gttcggcggc
      721 tagggcttgc cgcgtgacgg cgtctgcgac gcgacgccgt gccgcagcgg agtgcccggt
      781 tcggcggccg tcgcctcctg caccgtcgag ccggcgggcg tcggagtcac cccgggagcg
      841 ttcgactggt cgccgcctga cggctcggcg ccgtcgtgca gttccttgag ccgcgcgtgc
      901 aggatttcca gcaccggtat gcgattgccg tgttcggtct cgtggtcgaa cagccgccgc
      961 agcgggcctt cctccagcga gcggacccgg tgccgcaggt cgggcaggga gagttggtcg
     1021 tagtccggga tgggcagttc gtcgctgtga gccatgccta ccggttgccc accgctgccc
     1081 cgcggaaaac ggtcagctgc ccgtccactt cggcggccgc ttctcggcga aggccaccgc
     1141 gccctccctg gcgtcgttgg atgtgaagac cggcgccagg atcttgttct gcttcgtcca
     1201 catctcgtcg gggctccaac cgcgcgcctc gacgatgatg cgcttggtcg cggccaccgc
     1261 gagcgggccg ttgctgctga tccgctcggc cagcccgatc gccgcctcca gcgcagaacc
     1321 cggctcggcc aacaggttca ccagcccgag ctcgtgagcg cgctccgccg ggaggttctc
     1381 accggtcagc gccagctcca tcgcgaccgc gtacgggatg cgctgcggca gccgcagcaa
     1441 cccgccgccg cccgcgacca ggccacgctt gacctcaggg atgccgaacg ccgagtccct
     1501 cgacgccacg atcaggtcgg tggccagcgc cagctccgtc ccgccggcaa gcgcgtagcc
     1561 ctcgaccgcc gcgatgagcg gcttggcggg cgggcgttcg gtgaacccca tcccgcgacc
     1621 ctcgacgacg acgttctcgc cgcgcgcgaa cgccttgagg tccatgccgg cgcagaacga
     1681 accacctgcg ccggtgagga tgcccaccga gaggccgtcc tcgccgtcca gccggtccat
     1741 tgcgtcggcg agccccctgc tgaccgcggc gttcaccgcg tttttggctt tggggcggtt
     1801 gatggtgatg atcaggatgc ggtcgcgctg ttcgaccagc acggggcttt cgccgttggc
     1861 ttcttcgctc acgtcggtga tgctagcgac cagagtttgg cgcgctacgg cgactgggta
     1921 ctatgaaaac tagaacacgt tctaattcac cgagggggcc ccatggccac gaccgatatc
     1981 gagccgtcgg aactcaccaa gtgggatccg ggcctgaccg cgcgcgtgat ggggttgacc
     2041 cggcctttcc tgaagcgcta cttccgctcg gaggtgcgcg ggctggacaa cgtgccggcc
     2101 ggcggcgcgc tgctggtgtc gaaccactcc ggcggcatgc tgccgatgga cgtgccgatc
     2161 ctctcggctc actactacga gcagcacggc tacgaccggc cgctgtacac gctgagccac
     2221 gacatgctga tgatgggacc gacgggggac ttcttcaaga agatcggcta catcagcgcc
     2281 aaccacgaga acgccgacga ggcgctgcgg tccggcggcc tcgtcatcgt gtttcccggc
     2341 ggtgattacg acgtgtaccg accgacgctg tcggagaaca agatcgactt cggcggccgc
     2401 accggctata tcaaggctgc gctcaacgcc ggggttccga tcgtgcccac cgtcggcatc
     2461 ggggggcagg agacacagat cttcctgacc cgcggtacct ggctggccaa gcggctcgga
     2521 ccgatcgccc ggctggcgcg cgccaagatc gtgccgatct ccttcggttt cccgttcggc
     2581 ctgagcctgg tggtcccgcc gaacatcccg ctgcccacca agatcgtcat ggaggtactg
     2641 ccgcccatcg acatcgtcgc cgagttcggt gagaatcccg acatcgacga ggtcgacgcc
     2701 cacgtccgcc acgtcatgca gcgggcgctc gacgagctgg ccgccgaacg ccggctgccg
     2761 atcctgggct gaccctgatg gcgctcctgg acagactcaa cgacaccctg ggcctggtcg
     2821 ccaccatgcg gcgggcgggc atgatcgcgc cgctccggcc cgacaagtac gtgaagatcg
     2881 ccgcggcgat gcgtcgcgaa aacgtctcgg cggtctcggg ttttgcctcc gccgcgcaac
     2941 gctgccccga ccggtccggg ctcgtcgacg agctcggcac gctgacctgg cgcgagatcg
     3001 acgagcggtg tgacgccttc gcggccggcc tgcaggccct gcccggcggg cagccgcggg
     3061 tcatcggcgt catgtgccgc aaccatcgcg gcttcatcga ggccgtcgtc gcggccaacc
     3121 gcatcggcac cgacctgctg ctgctgaaca cctcgttcgc cgggcccgcc cttgccgagg
     3181 tggtcgcccg cgaaggcgcc gacgccgtcg tctacgacga ggagttcacc gagatcgtcg
     3241 ggcgtgcgct cgccgacgcg ccggacgcca cccgaatcgt cgcctggacc gacgaccctt
     3301 ccgcgctcgg tgacacgccg acggtcgaga agatgatcgc cgcgcatgcc ggccgcgaac
     3361 cgaagcgtgc cgcgcagaag ccgcggacga ttctgctgac gtcgggcacc accggtacgc
     3421 ccaagggcgc caagctcacc ggcggcgacc ccagtgcgct caaggcgata ctggaccgca
     3481 ccccctggca caccgaggaa gccatcgtca tcgcagcacc gatgttccac gcgtggggct
     3541 tctcgcagat gatcttcgcc gcgacgatgg cgtgcacgat cgtcacccgg cgcaaattcg
     3601 atccggaggc gacgttggag ttggtcgacc gccaccgcgc gaccgggctc gccgtggtgc
     3661 cggtgatgtt cgaccggatt atggaactgc cggacggggt acgcagccgc tacagcggac
     3721 ggtcgctgcg gttcgccgcc gcatcggggt cgcggatgcg gccggatgtg gtgac
//
LOCUS       DASYLX010000909         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_54271, whole
            genome shotgun sequence.
ACCESSION   DASYLX010000909 DASYLX010000000
VERSION     DASYLX010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54271"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VGP27_21225"
     CDS             <1..1677
                     /locus_tag="VGP27_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV7853856.1"
                     /translation="HRDDDGDFPDLGKGGNFYLTVNGRDFLVRGAAYTPDLLFAYDPD
                     REDAILRYVKDLGLNMVRLEGKFPGEHIVEKADELGIPLMFGWMCCNQWEKWSQWDDE
                     DRQVAQDSLRSQILMLRSHPSAFIWANGSDGKPPPDVLAKYHAILDDSHWQNATVDTV
                     SSLARDATGERDWDGIHMAGPYSWRPPGYWFSGRYGATRGSTAEQGDNEHIPPFESLK
                     KFIPPDKLWPINDTWYFHAGSNPKNATLQSIKRAVDRRYGPSDDAEEFSRKAQLAHYE
                     STRAQFEAFAANGWASHKMTIYWMLNNHWPSFFGNIFDYYLRPGGAYYGAKKGLQPLS
                     VVFDSYATGDHTKANVTLVNQTPNDRSDLRVRVRVYDLQGRMREDRMTDDLNVGSGGS
                     VEAMTLPRVARDSGVFFVRCEVVDGDGSVLAENVYWQSQQRDDLGDPANDWAFELRQV
                     SWADMTALNWMPKVPLQVSAKRSDAAGENGVTIRLQNPTSRVAFFERAEILGTRDGDE
                     ILPIEYSDNYVTVFPGETVEVHGVVPTDRTVANWVRVGGYNSPPVVVPIE"
     gene            1690..>2727
                     /locus_tag="VGP27_21230"
     CDS             1690..>2727
                     /locus_tag="VGP27_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV7853857.1"
                     /translation="MHEVDQNTEQMVRSVLAYAENRLRMDPVPLDKGVLPAEDLYERL
                     DGLIRDTPRQPDEVLGVYTSVIAPSVISADSPRFLGFIPAAPTKASLLFDMLVSCASI
                     QGISWLEASGAIAAENSVLRVIAHEAGLPETSGGCFVSGGSAGNLSALAAARETAKRH
                     GAAGRLRVVVGSDAHSSITNTLRLLEMDALVVETPDHRLTAQTVRDAVPPDAAGIAAV
                     VCTSGTTNAGIIDDLAGVGALARQRGWWFHVDGAYGGSGIFARSLRPKYAGLEQADSF
                     ILDPHKWLFTPFDCCALLYREPELARATHTQDASYLDVIHSGEGEWNPSDYAYHLTRR
                     ARGLPLWFSLAV"
BASE COUNT          496 a          894 c          907 g          430 t
ORIGIN      
        1 caccgcgacg acgacggcga cttccccgat ctgggcaagg gcggcaactt ctatctgacg
       61 gtcaacggac gcgacttcct ggtccgcggt gcggcctaca cacccgacct tctgttcgcc
      121 tacgacccgg atcgcgagga cgcgatcctg cggtacgtca aggacctcgg cctcaacatg
      181 gtgcgactgg agggcaagtt cccgggagag cacatcgtcg agaaggcgga cgaactcggg
      241 atcccgctga tgttcgggtg gatgtgctgc aaccagtggg agaagtggtc gcagtgggac
      301 gacgaagacc ggcaggtggc ccaggacagc ctgcggtcac agatcctgat gctgcgctcc
      361 cacccgtcgg cgttcatctg ggcgaacggc agcgacggca agccgccgcc cgacgtactc
      421 gcgaaatacc atgccatcct tgatgattcg cattggcaga atgccaccgt cgacactgtc
      481 tcctccctgg caagggatgc gacgggtgag cgcgactggg acggcatcca catggcgggg
      541 ccgtacagct ggcgaccacc gggctattgg ttcagcgggc gctacggcgc caccaggggg
      601 tccaccgccg aacagggcga caacgaacac attccgccgt tcgagagcct gaagaaattc
      661 atcccgcccg acaagctgtg gccgatcaac gacacctggt acttccacgc aggctcgaat
      721 ccgaagaacg ccacgttgca gagcatcaag cgcgcggtcg accggcgcta cggcccgtcc
      781 gacgatgccg aggagttcag ccgcaaagcc caactggcac actacgagtc gacgcgggcc
      841 cagttcgagg cgttcgccgc caacgggtgg gcgagccaca agatgaccat ctactggatg
      901 ctgaacaacc actggccatc gtttttcggc aacatcttcg actactacct gcgccctggc
      961 ggcgcctact acggcgccaa gaagggcctc cagccgctgt cggtcgtgtt cgactcctac
     1021 gccacgggcg atcacaccaa ggcgaacgtc acgctggtca accagacgcc aaacgaccgg
     1081 tcagacctgc gggtgcgcgt ccgcgtgtac gacctgcagg gcaggatgcg cgaggaccgg
     1141 atgaccgacg acctgaacgt cggctcgggc ggaagcgtcg aggcgatgac gttgcccagg
     1201 gtggcccgcg actccggcgt cttcttcgta cgctgcgaag tggtcgacgg cgacggaagc
     1261 gttcttgccg aaaacgtgta ctggcagtcg cagcagcgag acgatcttgg tgatccggcg
     1321 aacgactggg cgttcgaact gaggcaggtg agttgggcgg acatgaccgc gctgaactgg
     1381 atgccgaagg tcccgttgca ggtgagcgcg aagcgatccg atgcggcggg ggagaacggt
     1441 gtcaccatcc gcctgcagaa ccccacttcg cgggtggcct tcttcgagcg ggccgaaatc
     1501 ctcggcacgc gcgacggaga cgagatcctg cccatcgagt acagcgacaa ctacgtgacg
     1561 gtgtttcccg gcgagaccgt cgaggtgcac ggtgtcgtgc ccaccgaccg cacggtcgcc
     1621 aactgggtac gggtcggcgg atacaactcg ccaccagtgg tggtgccgat cgaatagagg
     1681 tgaggtgccg tgcacgaggt tgaccagaac accgaacaga tggtgcgcag cgtcttggcc
     1741 tacgcggaga accggctgcg gatggacccc gtgccgctgg acaagggagt gctgcccgcc
     1801 gaagacttgt acgagcggct cgacggcctg atccgcgaca caccgcgcca acccgacgag
     1861 gtgctcggcg tctacacctc ggtgatcgca ccgagcgtca tctcggcgga cagcccgcgc
     1921 ttccttggct tcatcccggc ggcaccgacg aaagccagcc tgctgttcga catgctggtg
     1981 tcgtgcgcgt cgatccaggg catctcgtgg ctcgaggcgt ccggcgccat cgccgccgag
     2041 aacagcgtgc tgcgggtgat cgctcacgag gcgggcctcc ccgaaacctc cggcggctgc
     2101 ttcgtgtcag gcgggtcggc gggcaatctc tcagctctgg cggccgctcg cgagaccgcc
     2161 aagcggcacg gcgccgccgg ccgtttacgg gtggtggtcg gctccgatgc gcactcgtcg
     2221 atcaccaata cgctgcggct gctggagatg gacgcgctgg tcgtcgagac accggatcac
     2281 cggctcaccg cccagaccgt ccgcgacgcg gtgccgcccg acgcggcagg cattgcggcg
     2341 gtggtgtgta cgtcgggcac caccaacgca ggcatcatcg acgacctggc aggggtgggc
     2401 gccctggcac gacagcgggg ctggtggttc cacgtcgacg gggcgtacgg aggctccggg
     2461 atcttcgccc gctcgctgcg accgaagtat gccgggctgg aacaggccga ctcgttcatc
     2521 ctcgacccgc acaagtggct gttcaccccg ttcgactgtt gcgcgttgct gtaccgggaa
     2581 ccagaactcg ctcgcgcgac gcacactcag gacgcgtcgt atctcgacgt catccacagc
     2641 ggggagggcg agtggaaccc gtccgattac gcctaccacc tgacccgccg cgcgcgtggc
     2701 ctgccgttgt ggttctccct cgcggtg
//
LOCUS       DASYLX010000910         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2140412,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000910 DASYLX010000000
VERSION     DASYLX010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140412"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1383)
                     /locus_tag="VGP27_21235"
     CDS             complement(13..1383)
                     /locus_tag="VGP27_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7853858.1"
                     /translation="MFPTTSRRLTGWGRTAPSVAKVLSTPDQELIAKAVAQAADRGAR
                     GVIARGLGRSYGDNAQNGGGLVVDMTVLNEIHSVDRESHLVDVDAGVNLDQLMRAALP
                     LGLWVPVLPGTRQVTVGGAIACDIHGKNHHSAGSFGNHVRSMELLTADGEVRHLTPDG
                     ADSELFWATVGGNGLTGIILRATIAMTPTETAYFIADGDVTAGLDETIAFHSDGTESN
                     YTYSSAWFDAISAPPKLGRAVISRGSLATVDQLPEKLRGNPLKFDAPQYFTTPDIFPN
                     GLGNKLIFGAMTELWYRTGKTYRGKVQNLTQFYHPLDMVGEWNRAYGAVGFTQYQFVV
                     PTEAVEPFKGIMYDIQRSGHYSFLNVFKLFGPGNKAPLSFPIPGWNVCVDFQVKPGLN
                     EFLNELDRRVLEFGGRLYTAKDSRTTAEVFHAMYPRIDDWIAVRRKVDPDGVFASDMA
                     RRLELL"
     gene            complement(1415..1813)
                     /locus_tag="VGP27_21240"
     CDS             complement(1415..1813)
                     /locus_tag="VGP27_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV7853859.1"
                     /translation="MQPNLSLTTQIWRFIVTGGFSAVVDFGLYVVLYRIAGMQPDLAK
                     TIGWIAGTTTAYLLNRRWTFQAPPSTARLAAVWALYITTFVVQVGLNHLFLRLLDYTP
                     TAVVVAFVIAQGTATVINFIVQRMVIFRLN"
     gene            complement(1978..3525)
                     /locus_tag="VGP27_21245"
     CDS             complement(1978..3525)
                     /locus_tag="VGP27_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7853860.1"
                     /translation="MPDTLYAQCDGLSLAYQVFGDGPIELVFGGSFASHIELYWTMPE
                     FVAFFERLSTFCRILLFDKSGVGLSDPVPRIRTLDERAAEIEAVMDTVGFERAVLFSG
                     SEGGPGSIVFAATRPERTRALILTGTFAYSFIDGWDDLDRDPAEVRMRLVRELGEAYS
                     PSVKQLVRWQAWCHAAVSAWGSGEAGKALLPSVRSIRQLAMVERMSASPGMARATFEA
                     MFRIDVRPILPTISAPTLLIHSRDEVVPVQCGRYIADHIPGARLLEVEGRDHVPWLAD
                     PDRILTEVEEFLTGSHAAPSPSRRALRTVVFTDMVASTQHAAATGDERWRAVLHRFDE
                     ITTELTQRFGGTVVEATGDGHLLTFDGPTQAIRCAETLRAEAETLGYEIRAGIHTGEC
                     ELRDNRIGGIAVHIAARIVDQAGPGEILVSRTVGDLVVGSGTGFEDRGSVELRGVPGI
                     WQLLAVDRKGPRAGSAEAELISTPTPGPGTAMRRSDRAVAAMAKRTPWILRGLARLAP
                     ATGRKST"
     gene            complement(3585..>4578)
                     /locus_tag="VGP27_21250"
     CDS             complement(3585..>4578)
                     /locus_tag="VGP27_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7853861.1"
                     /translation="TETTAVWGRLPAADAAVMKHRVTAMVNGLCDSDPRSAGERRSDA
                     AGAIANNNDTLACRCGSPDCPTAGVTPQSQVVIRVIADQAAIDAATTPPTQPAEQPAA
                     AQPAEQPPAQPAESAAAEKPAQPAEQPAEAARRPAAPALLLGHGVLPNALLAEAIRAG
                     ATIAPIRIPGAEPEPHYRPSDELAEFVRMRDLFCRFPGCTVTADRCDLDHARPWPWGP
                     THPSNLNCKCRPHHLMKTFWTGTGGWKDIQLPDATVIWTSPSGATYTTQPGSRLFYPN
                     WNITTADLPPPIEQPSPTGDRGLMMPRRQRPRAADIAAAIQAERALNQHDTAPF"
BASE COUNT          741 a         1423 c         1616 g          798 t
ORIGIN      
        1 atcgaacacc atttacagaa gctccaaacg tcgggccatg tccgaggcga acacgccgtc
       61 gggatcgacc ttgcgccgca cggcgatcca gtcgtcgatg cggggataca tggcgtggaa
      121 aacctcggcg gtggtgcggg aatccttggc ggtgtagagc cgcccgccga attcgagcac
      181 gcggcgatcg agttcgttga ggaactcgtt gagtcccggc ttgacttgga agtcgacgca
      241 gacgttccag ccggggatcg gaaagctcag cggcgccttg ttgccgggtc cgaacagctt
      301 gaacacgttg aggaaggagt agtgccccga acgctggatg tcgtacatga tgcctttgaa
      361 gggctccacc gcttcggtgg gcaccacgaa ctggtactgg gtgaaaccca cggcgccgta
      421 agccctgttc cactcaccga ccatgtcgag tggatggtag aactgggtca gattctggac
      481 cttgccgcgg taggtcttgc cggttcggta ccacagctcc gtcatcgccc cgaagatgag
      541 cttgttgcca agcccgtttg ggaagatgtc gggtgtggtg aagtattgcg gcgcatcgaa
      601 tttcagggga ttgccgcgca gcttctcggg aagctggtca acggtggcga gcgagccgcg
      661 ggagatcacc gcgcggccca gcttcggcgg cgcgctgatc gcgtcgaacc aggcgctgga
      721 ataggtgtag ttggactcgg tgccgtcgct gtggaaagcg atcgtttcgt cgaggcctgc
      781 ggtcacgtcg ccgtctgcga tgaagtacgc ggtctccgtc ggggtcatcg cgatcgtggc
      841 acgcaggatg atgcccgtca gaccgttgcc gccgaccgtc gcccagaaca actctgagtc
      901 ggcgccgtcc ggcgtcaggt ggcggacctc gccgtcggcg gtcagcagtt ccatcgaccg
      961 cacatggttg ccgaagctgc ccgcgctgtg gtggttcttg ccgtgaatgt cgcaggcgat
     1021 ggcgccgccg accgttacct ggcgggtgcc gggcagcacg ggcacccaca gaccgagcgg
     1081 tagcgctgcc cgcatcagct gatcgaggtt cacgcctgcg tcgacgtcga ccaggtggct
     1141 ttcgcgatcg accgagtgga tttcgttgag caccgtcatg tcgacgacga ggccgccgcc
     1201 gttctgggcg ttgtcgccgt acgaccggcc gagaccccgg gcgatgacac cgcgggcgcc
     1261 cctatcggcc gcctgcgcga cggccttggc gatcagttcc tggtcgggtg tcgagagcac
     1321 cttggccacc gacggggcgg tgcggcccca gccggtcagg cgtcgtgagg tggtcggaaa
     1381 catcgtgacg agggtaccgt gcggttccat cggctcagtt caagcggaag atcaccatcc
     1441 gctggacgat gaagttgatc acggtcgcgg tgccctgggc gatgacgaat gccaccacca
     1501 ctgcggtcgg tgtgtagtcg agtagccgca ggaacagatg gttgagtccg acctggacga
     1561 cgaaggtcgt gatgtagagc gcccacaccg cggccaacct cgctgtgctg ggcggagcct
     1621 ggaaggtcca ccgccggttc aacaggtatg cggtcgtggt tccggcgatc catccgatgg
     1681 tcttggcgag gtcgggttgc atcccggcga tgcggtacag cacgacgtac agcccgaaat
     1741 cgacgacggc cgagaacccg ccggtgacga tgaaccgcca tatctgggtc gtgagactca
     1801 ggttcggctg catcgggggc tcggtcacgc gggcagccta ctggggccgg acaccgaaac
     1861 tcgacccgct gctcgactta cacttcggca atgttctccg agacgcgcta tgcgctgaac
     1921 ggggacttgc gcgccgcgta tcgcgcgtca cggtcgcatc tttttagctc aggttattta
     1981 ggttgatttg cgcccggtgg ccggcgcgag ccgggccaac ccgcgcagga tccacggtgt
     2041 gcgcttggcc atcgcggcca cagcacggtc ggagcggcgc atcgcggtgc cagggccggg
     2101 ggtcggcgtt gaaattagtt ccgcctccgc cgatcccgcg cgcggccctt tgcgatcaac
     2161 cgccagcagt tgccagatgc cgggcacgcc gcgaagctcg acgctgccgc ggtcctcgaa
     2221 ccccgtaccc gagccgacga ccaagtcgcc cacggtgcgg gagacgagga tttcaccggg
     2281 cccggcctgg tcgacgatcc tggccgcgat gtgtaccgcg atcccgccga tccggttgtc
     2341 ccgcagttcg cactcgccgg tgtgaatgcc agcgcggatt tcataaccca gtgtctcagc
     2401 ttcggcacgc aaggtctcgg cgcagcggat cgcctgagtc gggccgtcga atgtcaggag
     2461 gtgaccgtcg cccgtggcct ctaccaccgt gccgccgaat cgctgggtga gctctgtggt
     2521 gatttcgtcg aaccggtgca gcaccgcacg ccaccgctcg tcgccggtcg ccgcggcatg
     2581 ttgcgtcgac gcgaccatgt cggtgaacac cactgtgcgc agcgcgcgac gtgacgggga
     2641 cggcgcggcg tggctgccgg tgaggaactc ctcgacctcg gtcaggatcc ggtcgggatc
     2701 cgcgagccac ggcacgtggt ctctgccctc gacttcgagc aacctcgcac cggggatgtg
     2761 gtcggcgata taccggccgc actgcaccgg aacgacttcg tcgcgggaat ggatgagcag
     2821 tgtgggagcg ctgatcgtcg gcaagatggg ccgcacgtcg atccgaaaca tcgcttcgaa
     2881 tgtcgcccgc gccatccctg gactggcgct catgcgttcc accattgcga gctgacggat
     2941 cgaccgcaca gacggcagca gagccttgcc cgcctcgcca cttccccacg ccgaaacagc
     3001 ggcgtggcac caagcctgcc agcggacgag ctgcttcaca gagggcgagt aggcctcacc
     3061 cagctcgcgc acgaggcgca tccggacctc ggcggggtcg cggtccagat catcccagcc
     3121 gtcgatgaag gagtacgcaa acgtgccggt gagaatcaac gcacgcgttc gctccggtcg
     3181 tgttgccgcg aaaacgatag accccgggcc gccctcgctc cccgagaaca gcactgctct
     3241 ctcgaagccg acggtgtcca tgacggcctc gatctcggcc gccctttcat ccaacgtgcg
     3301 aatacgcggg accggatccg acagcccaac cccggacttg tcgaacaaaa ggatgcgaca
     3361 gaaagtggag agccgctcga agaaggccac aaactcgggc atcgtccagt acagctcgat
     3421 atggctggca aacgacccgc cgaaaaccag ctcgatcggt ccgtcgccga aaacctgata
     3481 cgccaggctt agaccgtcac actgtgcgta caacgtatcc ggcacgggcc tgagcgtact
     3541 gcggccgggc gctggcccca ccgtatccac cgcattgcaa ctgctcagaa gggcgcggtg
     3601 tcgtgttggt tgagtgcgcg ttcggcttga attgccgcgg cgatgtcggc ggctcggggg
     3661 cgttgtcggc gcggcatcat cagcccgcga tcgccagtgg gggatggctg ttctatcggt
     3721 gggggcaggt cggcggtggt gatgttccag ttggggtaga acagtcggct gccgggttgg
     3781 gtggtgtagg tggcgccgct gggcgaggtc cagatgacgg tggcgtcggg cagttgaatg
     3841 tctttccagc cgccggtgcc ggtccagaag gttttcatca gatggtgtgg gcggcatttg
     3901 cagttcaggt tggaggggtg ggtggggccc cacggccacg gccgggcatg gtcgaggtcg
     3961 cagcggtcgg cggtgacggt gcagccgggg aaacggcaga acagatcgcg catccgcacg
     4021 aactcggcca gctcgtcgct gggccgatag tggggctcgg gttcagcacc gggaatgcgg
     4081 atcggtgcga tggtggcgcc ggcgcgaatc gcctcggcca gcagcgcatt gggcagcacc
     4141 ccgtgaccta acagcagcgc cggcgccgcc ggccgccggg cggcctcggc gggctgctcg
     4201 gcgggttggg cgggcttttc agcggcggcc gactcggcgg gctgggcggg tggttgctcg
     4261 gcgggctggg cggcggcggg ctgctcggcg ggttgggtgg gtggggtggt ggcggcgtcg
     4321 atggcggctt ggtcggcgat cacccggatc accacctggg attggggggt caccccggcg
     4381 gtggggcagt ctggtgatcc gcatcgacag gccaaggtgt cgttgttgtt ggcgatcgcg
     4441 ccggcggcat cggagcggcg ttcgccggcg ctgcgcggat cgctgtcgca cagcccgttg
     4501 accatcgcgg tgacgcggtg tttcatcact gcggcgtcgg cggccggcag ccgcccccac
     4561 accgcagtgg tctcggtg
//
LOCUS       DASYLX010000911         6944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1700207,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000911 DASYLX010000000
VERSION     DASYLX010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700207"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGP27_21255"
     CDS             complement(<1..414)
                     /locus_tag="VGP27_21255"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV7853862.1"
                     /translation="MAIVNRTPDSFYDRGATFTDDAAKTAAHRAIADGADVVDVGGVK
                     AGPGEDVGVDAEIARVVPFIEWLRDSYPDQVISVDTWRSAVAKQACAAGADLINDTWG
                     GHDPGLAEVAAEFGAGLVCSHTGGATPRTRPFRVNY"
     gene            complement(539..2347)
                     /gene="fadD6"
                     /locus_tag="VGP27_21260"
     CDS             complement(539..2347)
                     /gene="fadD6"
                     /locus_tag="VGP27_21260"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase FadD6"
                     /protein_id="HEV7853863.1"
                     /translation="MTDEKSGTRSSVGLLDIASRVPGLIADAPTILRGVITGFGARPS
                     AKTSIGKVFQDRAAQYGKKVFLKFDDQEITYREANETVNRYAAVLAARGVGHGDVVGI
                     MLRNSPDPVMLMLAAVKCGAISGMLNYHQRGDVLKHSLGLLSATILIADPDFVEPIKE
                     SGADTDAMVTLGEFKKLAETAPTENPPTASAVLAKDKAFYIFTSGTTGMPKASIMTHY
                     RWLRALAGFGGLGMRLNSNDTLYCCLPLYHNNALTVALSAVLNGGSTLALGKSFSASK
                     FWDEVIRYGATAFVYIGEICSYLLNQPEKDTDRKHKVRVIAGNGLRPAIWDRFTERFG
                     IDRVCEFYAASEGNTAFVNVLNVDKSTGICPTPVAFVEYDEESGDPKRGDDGRVRKVK
                     NGEPGLLLSKVSSFQPFDGYTDKEESEKKLVRDAFKEGDVWFNTGDLMRSQGFGHAAF
                     TDRLGDTFRWKGENVATTEVEAAVSTDSQIEEATVFGVEVPDTGGRAGMVAIQLKDGQ
                     EFDGKALAKAAYDNLPSYAVPLFVRVVKELAHTSTFKSQKVDLRKEGYGGDSGEGDED
                     AGEIEDPIYVLAGRDEGYVEFYDDYLDEVKDGKKPK"
     gene            complement(2409..2969)
                     /locus_tag="VGP27_21265"
     CDS             complement(2409..2969)
                     /locus_tag="VGP27_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV7853864.1"
                     /translation="MSADPDSEWAVCVYCASGPKHPALLDLASRVGGAIAERGWTLVS
                     GGGNVSAMGAVATGARARGGRTIGVIPKALVHRELADVDADELVVTETMRERKQVMED
                     RADAFLALPGGIGTLEEFFEAWTAGYLGMHDKPVVMLDPFGHYDGLRAWLHGLIDSGY
                     VSQTALDRLVVVDDIDDALAACAPAR"
     gene            3028..5112
                     /locus_tag="VGP27_21270"
     CDS             3028..5112
                     /locus_tag="VGP27_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7853865.1"
                     /translation="MPVEWKTAPPQPAAERVESAVAHRGGAVVVAPVGAGKTTVAGLA
                     SERIGGPTAWVTGTVPGRVVPFAALRDLIEVPDDGKTAAVLRAARESVADGLLLIVDD
                     AHLLDHLSATLVYQLAVGGAVRLIVTVDPDEAAPDSISALQLHNVLPRIELDPPSVER
                     KRLDASVREFVGSLPSGARVALEFLAVADPISLADLSALAGEEAVRAAEAADAVTVVD
                     DAARAAHPLYTDAARAQMSDDDLHARRTALYEMLSARNPRDVVDRLRLAVLALDSGVS
                     QPVSEVVAAAADALRLGDLPLSERLGEAVLARDPDLDARLTLAQALAWQGRGREADEV
                     LAAVDPASLSDTELMAWALPRAANQFWMLSEPERATAFLQTTRHRVTSPAARTTVDAL
                     SATFAMNAGRPGRAMELAAEVLASPDADDTAIGWSAAAAALSCARMGRFADVDALAER
                     AAEAQHPGLLRFTSGFGQTTALLMAGELDRAQELAQQLADFAQMQQPGRAIGEILVAD
                     VLIARGESAAAAKLLREAAEALAPTGYSWGPLAWMLLAQALGQQGEMGEASKTLARAE
                     SRHGLKSMLFAPELQLARAWTTMARRDPAGARSAARDAGRAAERGGQSAIAVRAFHDA
                     VRLGDIRAVDSIARLIDDVDCVVGRLVLRHARAFVEGHADELEAVSAAFADIGMMAAA
                     ADASAQAKAAAR"
     gene            complement(5133..6728)
                     /locus_tag="VGP27_21275"
     CDS             complement(5133..6728)
                     /locus_tag="VGP27_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV7853866.1"
                     /translation="MSETSIVCSNLSFSWPGEKALLEELSFTVGDGRTGLVAPNGAGK
                     STLLKLIAGEYRPSAGSATVEGSLGYLPQTLPFDSRRTVAEVLGVAPILEALNALACG
                     DAGDAVFAAIGDDWDAEERGRAQLDRVGLGSVALDRRLGSLSGGEVVSLGLAAELFKR
                     PDVLLLDEPTNNLDAEARAKLYDVLDDYKGCLLLVSHDRMLLDRMDRIAELHRGEIAI
                     CGGNFAAYEESVREAQRVAESSVRNAEQQLRREKRQMQQARERAARRSRTAARNIKDA
                     GLPKIIAGAMKRRAQESAGRIDGVNAGRVDDARARLDDAEGALRDDSSIALDLPDTEV
                     PAGRDVFSGNGIRMSRGGRALFGDGLDVSIRGPERIALTGPNGAGKSTLLRIIAGELQ
                     PDDGTVRRADGRIAYLSQRLDLLDPDRTVLQSLVAYAPNLSETRRMHLLAQFLFRGNM
                     IHLPVSALSGGERLRVTLACVLFAEPAPQLLLLDEPTNNLDLASIGQLESALNAYRGA
                     FVVVSHDARFLEKVRLDRTMRLP"
     gene            complement(6828..>6944)
                     /locus_tag="VGP27_21280"
     CDS             complement(6828..>6944)
                     /locus_tag="VGP27_21280"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970904.1"
                     /note="catalyzes the formation of succinate and
                     diaminoheptanedioate from succinyldiaminoheptanedioate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEV7853867.1"
                     /translation="PAVNFGPGDPNLAHRVDERVEVRQITAATDMLRRYLTA"
BASE COUNT         1013 a         2327 c         2430 g         1174 t
ORIGIN      
        1 gtagttgacc cgaaacgggc gggtgcgcgg cgtcgcgcca ccggtgtggg agcacaccag
       61 cccggcaccg aactccgcgg cgacctcggc caggccgggg tcgtggccgc cccaggtgtc
      121 gttgatcagg tcggctcccg ccgcgcaggc ctgtttggcg acggcgctgc gccaggtgtc
      181 gacgctgatg acctggtcgg gatagctgtc gcggagccat tcgatgaacg gcaccacccg
      241 cgcgatctcc gcgtcgacgc cgacgtcctc gccgggtccg gccttgaccc cgccgacgtc
      301 cacgacgtcg gcaccgtcgg cgattgcgcg atgagctgcg gttttagccg cgtcatcggt
      361 gaaggtggcg ccccggtcgt agaacgaatc cggggtgcgg ttgacgatcg ccatgatcag
      421 cgcacgatcg ccggccaccg gacggccgca gaagatcgac tgcaccctta caggctacgg
      481 acagggtgat ttcggcgcgc tggcgtgcgc tctgcgtcga tgagcacgcc caaatcgcct
      541 acttcggctt tttgccgtcc ttcacttcgt cgaggtagtc gtcgtagaac tcgacataac
      601 cctcgtcgcg gccggccagc acgtagatcg ggtcctcgat ctcgccggcg tcttcgtcgc
      661 cttccccgga gtcaccgccg tagccctcct tgcggaggtc gaccttctgg ctcttgaagg
      721 tcgacgtgtg cgcgagttcc ttgacgacgc ggacgaacag tggcacggca taggacggca
      781 gattgtcgta ggcggccttg gccagggcct tgccgtcgaa ctcctggccg tccttgagct
      841 gaatggccac cattccggcg cggccgccgg tatcgggcac ctcgacgccg aacaccgtcg
      901 cctcctcgat ttgcgagtcg gtcgacaccg cggcctcgac ctcggtggtg gcgacgttct
      961 cgcccttcca gcggaaggtg tcgccgagac ggtcggtgaa cgccgcgtgg ccgaagccct
     1021 gcgagcgcat caggtccccg gtgttgaacc agacgtcgcc ctccttgaag gcgtcgcgga
     1081 ccaatttctt ctcggattcc tctttgtcgg tgtaaccgtc gaatggctgg aagctgctga
     1141 ccttcgagag caacagcccc ggctcgccgt tcttgacctt gcggacccgg ccgtcgtcgc
     1201 cgcgcttcgg gtcgccggat tcctcgtcgt actcgacgaa cgccaccggc gtcgggcaga
     1261 tgccggtgct cttgtcgacg ttgaggacgt tgacgaacgc ggtgttgccc tcgctggcgg
     1321 cgtagaactc gcacacccgg tcgatgccga accgctcggt gaaccggtcc cagatggccg
     1381 gcctcagccc gttgcccgcg atcacccgca ccttgtgctt gcggtcggtg tccttctccg
     1441 gctggttgag caggtaggag cagatctcgc cgatgtacac gaaagccgtt gcgccgtagc
     1501 ggatcacctc gtcccagaat ttcgacgccg agaacgactt gccgagcgcc agcgtcgagc
     1561 ctccgttgag caccgccgac agcgcgaccg tcagcgcgtt gttgtgatag agcggcaggc
     1621 agcagtacag ggtgtcgttg ctgttgagcc gcatgccgag gccgccgaag ccggcgagcg
     1681 cgcgcagcca gcggtagtgc gtcatgatgc tggccttcgg cataccggtg gtgcccgagg
     1741 tgaagatgta gaacgccttg tccttggcga ggacggccga cgcggtgggc ggattctcgg
     1801 tgggcgccgt ctccgcgagc ttcttgaact cgccgagggt caccattgcg tcggtgtcgg
     1861 cgccgctttc cttgatcggc tcgacgaagt cggggtcggc gataaggatc gtggcggaca
     1921 acaacccgag gctgtgcttg agcacgtcac cgcgctggtg gtagttcagc atgccggaga
     1981 tggcgccgca cttgaccgcg gcgagcatga gcatcaccgg gtcgggcgag ttgcgcagca
     2041 tgatgccgac gacgtcgccg tgtccgacgc cgcgtgcggc gagcaccgcg gcgtagcggt
     2101 tgacggtctc gttggcctcc ctgtaggtga tctcctggtc atcgaatttc aggaagacct
     2161 ttttgccgta ctgggcggcc cggtcctgga acaccttgcc gatggaggtc ttggccgacg
     2221 ggcgggcccc gaacccggtg atcacgccgc gcaggatcgt cggcgcgtcg gcgatgaggc
     2281 cgggtacgcg gctggcgatg tcgagcagcc cgacgctgct acgggtccca gatttctcgt
     2341 cggtcatgcg gcgatccccc tccggtgtcg tcttgcccga caccctaagt ccctgcgggt
     2401 gaccgccatc accgggccgg tgcgcaggcc gccagcgcat catcgatgtc gtccaccaca
     2461 acgagccgat ccaatgccgt ctggctgacg tagccgctgt cgataaggcc gtgtagccag
     2521 gcacgcagcc cgtcgtaatg cccgaacgga tcgagcatca ccaccggctt gtcgtgcata
     2581 cccagatagc ccgctgtcca cgcttcgaag aactcctcga gggtgccgat gcccccgggc
     2641 aacgcgagga acgcgtcggc gcggtcctcc atcacctgct tgcgttcccg catcgtctcg
     2701 gtgacgacga gttcgtcggc gtcgacgtcg gcgagctcgc gatgtaccag cgccttgggg
     2761 atcaccccga tggtgcggcc accgcgcgct cgggcgccgg tggccacggc acccatggcc
     2821 gagacgtttc ccccgccgga caccaacgtc cagccgcgct ccgcgatggc gccgccgaca
     2881 cggctcgcca ggtcgagcag cgcggggtgt ttcggccccg acgcgcagta cacgcagacg
     2941 gcccattcgg agtcgggatc ggctgacaca acgtcaacgt agacgagtcc ggcacggtcg
     3001 ccgcggccac accctaaact ccggcgggtg cccgtcgagt ggaagaccgc gccgccgcaa
     3061 ccggcggccg agcgagtcga gtcggcggtg gcgcaccgcg gcggcgcggt ggtcgtcgcg
     3121 cccgtcggtg caggtaagac gactgtggcc ggcctggcca gtgaacgcat cggcgggccg
     3181 accgcgtggg tgaccgggac ggtgccgggt cgcgtcgtcc cgttcgcggc gctgcgcgac
     3241 ctgatcgagg tccccgacga cggcaagacg gcggccgtac tgcgcgccgc ccgcgagtcg
     3301 gtggccgacg ggctgctgct gatcgtcgac gacgcgcatc tgctcgacca tctgtccgcg
     3361 acgctggtct atcagctggc ggtcggcggt gcggtgcggc tgatcgtcac cgtcgacccc
     3421 gatgaagccg caccggattc gatctcggcg ctgcagctgc acaacgtgct gccgcggatc
     3481 gagctcgacc cgccgagcgt ggagcgcaag cggctcgacg catcggtgcg ggagttcgtc
     3541 ggttcgcttc cttcgggcgc acgggtggcg ctggagtttc tggccgtcgc cgacccgatc
     3601 tcgttggccg acctgtcggc gttggcgggt gaagaggctg tgcgggccgc cgaggccgcc
     3661 gacgcggtta cggtcgtcga cgacgccgcc cgggcggccc atccgctcta caccgatgcg
     3721 gcccgtgcgc aaatgtcgga cgacgatctg cacgcgcggc ggaccgcgct ctacgaaatg
     3781 ttgtcggccc gcaaccctcg ggatgtcgtc gaccggttga gactggccgt gctggcgctg
     3841 gacagcggtg tttcgcaacc cgtttccgag gtggtggccg ccgcggccga cgcgctgcgg
     3901 ctgggcgacc tgccgttgag cgaacggctc ggcgaggcgg tgctcgcgcg cgaccccgac
     3961 ctggatgcgc ggctgacgtt ggcccaggcg ttggcgtggc aaggccgcgg ccgtgaggcc
     4021 gacgaggtgc ttgccgccgt cgatccggcc agcctgtcgg acaccgagtt gatggcgtgg
     4081 gccttaccgc gcgcggccaa tcagttctgg atgctgtccg agccggagcg ggcgacggcg
     4141 tttctgcaga ccactcgcca tcgcgtcacg tcgccggcgg cgcggaccac cgtggacgca
     4201 ctgtcggcga cgtttgcgat gaacgccggg cgtcccggac gggcgatgga gcttgcggcc
     4261 gaagtgcttg cctcgccgga cgccgacgac accgcgatcg gctggtcggc cgcggcggcg
     4321 gcgctcagct gtgcccggat gggccggttc gccgacgtcg acgcgctggc cgagcgggcc
     4381 gccgaggcgc agcatcctgg tctgctgagg ttcaccagcg ggttcggcca gacgacggcc
     4441 ctgctgatgg ccggcgagct ggaccgcgct caggaactcg cgcaacaact cgccgacttc
     4501 gcgcagatgc agcagccggg ccgggcgatc ggcgagattc tggtggccga cgtgctgatc
     4561 gcccgcggtg agtcagcggc ggcggcgaag ctgctgcgtg aggccgccga ggcattggcg
     4621 ccgaccggtt attcgtgggg gccgctggca tggatgctgc tggcccaggc gctcggtcag
     4681 cagggcgaga tgggcgaagc gtcgaagacg ttggcgcggg cggagtctcg acacgggctc
     4741 aaatcgatgc tgttcgcgcc cgagctgcag ttggcccggg cgtggacgac gatggcgcgc
     4801 cgtgacccgg caggggcgcg gtctgcggcc cgcgatgcgg gacgggcggc ggagcgcggc
     4861 ggccagtcgg cgatcgcagt gcgtgcgttc cacgatgccg tccgactcgg cgacatccgt
     4921 gcagtcgact ccatcgcccg gctcatagac gacgtcgact gcgttgtcgg caggctggtg
     4981 ttgcggcacg cccgcgcctt cgtcgagggc catgcggatg agttggaggc ggtgtcggcc
     5041 gcgtttgcgg acatcggcat gatggccgcg gccgccgacg cctcggctca ggccaaagcc
     5101 gccgcccgct gaccttctcc gcgaaagcga agtcagggca gtcgcatcgt ccggtccagg
     5161 cggacctttt cgaggaaccg tgcgtcgtgg ctgacgacga cgaacgcgcc gcggtaggcg
     5221 ttcagggcgg attccagttg tccgatgctg gcgagatcga ggttgttggt cggctcatcg
     5281 aggagcaaga gctgcggcgc cggttcggcg aacagcacgc acgccagtgt gacgcgcagc
     5341 cgctcaccac ccgacaatgc cgacaccggc agatgaatca tgttgcctcg gaacaggaat
     5401 tgtgccaaca ggtgcatccg tcgcgtctcg ctcagattcg gcgcgtatgc gacgagactc
     5461 tggagcacgg tccggtcagg atcgaggagg tcgagacgct gcgacaggta ggcgatccgc
     5521 ccgtcggccc gccggacggt gccgtcgtcc ggttggagtt ctcccgcgat gatgcgcagc
     5581 agcgtcgact tgcccgcgcc gttcggcccg gtgagcgcaa tgcgctcggg cccgcggatc
     5641 gagacgtcga gcccgtcgcc gaacaaggca cggccccctc ggctcatccg tattccgttg
     5701 cccgagaaca cgtcgcggcc ggcgggcacc tcggtgtcgg gcaggtcgag cgcgatcgac
     5761 gagtcgtcgc gcagcgcgcc ttcggcgtcg tcgagcctgg ccctggcgtc gtcgaccctc
     5821 cccgcgttca cgccgtcgat gcggccggcc gactcctgag ccctgcgctt catcgcgccg
     5881 gcgatgatct tcggcagccc ggcgtctttg atgttgcgtg ccgcggtgcg cgaccggcgg
     5941 gctgcgcgtt cgcgtgcctg ttgcatttgc cgcttctcgc gcctgagctg ttgctcggcg
     6001 ttgcggacgc tgctctcggc gacgcgctgc gcctcgcgca ccgactcctc gtacgcagcg
     6061 aaattgcctc cacagattgc gatctcgcca cggtgtaact cggcgatccg atccatccgg
     6121 tcgagcagca tgcggtcgtg gctgaccaga agcagacatc ctttgtagtc gtcgagtacg
     6181 tcgtagagct tcgcgcgtgc ttcagcgtcg aggttgttgg tcggctcgtc gagcagcagc
     6241 acatcgggcc gcttgaacaa ttcagcggcc agcccgagcg agaccacctc gccgccgctc
     6301 agcgatccga gcctgcgatc gagcgcgacg ctccccaggc cgactcggtc gagttgggcg
     6361 cggccgcgtt cctcggcgtc ccagtcgtcg ccgatcgcgg cgaagacggc atcgcctgcg
     6421 tcgccacagg caagcgcgtt cagcgcctcg aggatcggtg cgacgccgag cacctcggcc
     6481 acggtgcgcc gggaatcgaa cggcaaggtc tgtggcagat aaccgagcga tccttcgacg
     6541 gtcgccgaac ccgccgacgg ccggtattcg cctgcgatca gcttcagcag cgtgctcttg
     6601 cctgccccgt tgggcgccac caaacccgtg cggccgtcgc cgacggtgaa ggacaactcc
     6661 tccaacagcg ccttctcgcc gggccacgag aaggacagat tcgaacagac aatggatgtt
     6721 tcagacatgt gagggctctt cgtgtaggca cggaaaaagg cgacagcatc gatccggggt
     6781 cacccggaga tggcgccaac tcacatgcct tcaggctatg cgtcgagtca agccgttaaa
     6841 tatcgccgca gcatgtcggt ggccgcggtg atctggcgca cctcgacccg ctcgtcgacg
     6901 cggtgcgcga ggttcggatc gccgggtccg aagttgacgg cggg
//
LOCUS       DASYLX010000912         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1700293,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000912 DASYLX010000000
VERSION     DASYLX010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700293"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="glpR"
                     /locus_tag="VGP27_21285"
     CDS             <1..441
                     /gene="glpR"
                     /locus_tag="VGP27_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HEV7853868.1"
                     /translation="SMMAARRRRYDPAKAAAISARKYKFRKRMLAFMTLMMMASAAAA
                     YFMQPMGWYLCGGAGAVTLLYLGYLRRQTRIEERLRRRRAKRAARARLGVENTEDEDL
                     DLVPSRLRRPGSVVLEIDDEDPIFEHLEYTPVARGYDLPRAAGQ"
     gene            505..580
                     /locus_tag="VGP27_21290"
     tRNA            505..580
                     /locus_tag="VGP27_21290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:538..540,aa:Ala,seq:cgc)
     gene            complement(772..873)
                     /locus_tag="VGP27_21295"
                     /pseudo
     CDS             complement(772..873)
                     /locus_tag="VGP27_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917703.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            947..1324
                     /locus_tag="VGP27_21300"
     CDS             947..1324
                     /locus_tag="VGP27_21300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853869.1"
                     /translation="MAADKSHLVLLHGIGSSSESAWREVVPLLADHHQVYAPAALGHR
                     GGPPVERRPVTMTDLVEAAEQYLDERGLDRPHLAGSSMGAFVAIELARRGRAASVCAL
                     SPPGLWSSGDVYKLVPSKRFEGT"
     gene            1267..1731
                     /locus_tag="VGP27_21305"
     CDS             1267..1731
                     /locus_tag="VGP27_21305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7853870.1"
                     /translation="MVFRGCLQARAFKTIRRNLRLGRLMRPAYPLMFRSASLRRIAMR
                     DVAVHGDRLTPGQAVKRAKDSLDCEVPDVANDLSLDDFHIAPLTPLPCPITVAWSGED
                     ALLPMAEYASALSERLPEAAFSVLAGVGHEPMLDNPELVARTILHVTNAARK"
     gene            1851..2303
                     /locus_tag="VGP27_21310"
     CDS             1851..2303
                     /locus_tag="VGP27_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7853871.1"
                     /translation="MDVAMWFEKRVGMPLLNLHDRIYKSTNGRIGHRIPGAPPSLLLH
                     TIGAKTGQQRTNSLTYARDGDSYLVVASKGGDPKAPGWYHNAKANPGVEINVGPKRLP
                     VTARPVLPDDTSYDRLWEIVNKNNSNRYKNYQTRTSRPIPVVVLTPRV"
     gene            2498..2854
                     /locus_tag="VGP27_21315"
     CDS             2498..2854
                     /locus_tag="VGP27_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853872.1"
                     /translation="MTTPSPPPGWHPDPSGAPGSATSTAPSGQNTDPGHPLVSGYPAR
                     SVRAGWMPQSLSASAMAMKSVTEAIVKGDPNGWRAVAEMAENKSGRTTSALWFALQDR
                     RVGGPRHGNNKKEGDA"
BASE COUNT          588 a          878 c          919 g          513 t
ORIGIN      
        1 tcgatgatgg ccgcgcggcg gcgccgctac gacccggcga aggcagcggc gatcagcgcg
       61 cgcaagtaca aattccgcaa gcggatgctg gcgttcatga cgctgatgat gatggcgtcg
      121 gcggcagccg cgtacttcat gcagccgatg gggtggtacc tctgcggcgg tgccggcgcg
      181 gtgacgctgc tctacctggg ctatctgcgc aggcagacgc gcatcgagga acggctgcgg
      241 cgacgcaggg ccaaacgggc ggcacgtgcg cgcctcggcg tggagaacac cgaggacgag
      301 gacctcgacc tggtcccgtc gcggctgcgt aggccgggct cggtggtgtt ggagatcgac
      361 gacgaggacc cgatcttcga gcatctggag tacacaccgg tggcccgcgg ctacgacctg
      421 ccgagggcgg ccgggcagta attcggcgtc ccgtctgccg gtttctgccg cgggggattg
      481 actggtagcc tgtgctaccg accaggggct atggcgcagt tggtagcgcg actcgttcgc
      541 atcgagtagg tcaggggttc gattcccctt agctccacca gttttgatgc agttcagggt
      601 gctaaagccg attggctact gtcaaggctc aagatgggca acccgcagtc agcccgcagg
      661 agaacccgca gagctgacta gcggaggcca cgcagctgac cgaaacgccg ccggtagcaa
      721 actcattggc tcgcatcctt gcggggcgcc tcggcggtca tctgtcattg cacgcacgcc
      781 gcctggctgc cggaagctga gtgcggtttt gacctcgtcg acttttggcg acgtccggcc
      841 cgccaagtcg gccagcgtct aggcgactgc gagggcaaca aaatagcgga acttgcgaac
      901 taaactaact gcgccctccg cggcgagatt gtgcgaagct ccgcaaatgg cagctgataa
      961 gtcgcacctg gtgttgcttc atggaatcgg ttcgtctagc gaaagcgcgt ggcgagaagt
     1021 agttccgctt ctcgcggacc atcaccaggt gtatgcacct gctgctctag ggcatcgcgg
     1081 tggcccacca gtggagcgac gtcctgtcac gatgactgat ctcgtggaag ctgctgagca
     1141 atacctggac gagcgcggcc ttgaccgacc gcatctcgct ggcagctcca tgggtgcctt
     1201 tgtggctatt gagttggcgc ggcgcggccg ggcggcatcg gtgtgtgcct tgtcccctcc
     1261 cggcctgtgg tcttcagggg atgtctacaa gctcgtgcct tcaaaacgat tcgaaggaac
     1321 ctaaggttgg gccgccttat gcgacctgcc tacccgctga tgttcagatc ggcctctcta
     1381 cgcagaatcg cgatgcgcga cgttgccgtt catggggatc gcctgacgcc cggccaagcg
     1441 gtcaagaggg ctaaagactc gcttgactgc gaagtccccg atgtggccaa tgatctgtcg
     1501 cttgacgact ttcacatcgc cccgctgacc cccttgccgt gcccaattac tgtcgcatgg
     1561 tcgggggaag acgcgctgct tccaatggcg gaatatgcaa gcgcactttc ggagcgccta
     1621 cccgaggccg cgtttagcgt gctcgccggt gtcggccacg aaccgatgct tgacaacccc
     1681 gagttggtgg cgcgcaccat ccttcacgtc acaaacgcag ccaggaagta gtcccaagag
     1741 ggccgaaccc gggtcggagt acgccagcgt gctcgtcaca gccaacgtaa ccagatcgca
     1801 gatgtagggg aactgctgcg cactcgacgg gtgtcaacca gactgtcgac atggatgtcg
     1861 cgatgtggtt tgaaaaacgg gtcggcatgc cgctgctgaa cctccacgac aggatctata
     1921 agagcaccaa tggtcgaatc gggcaccgca tacccggcgc tccgcccagc ttgctgttgc
     1981 acaccatcgg agcgaagaca gggcagcagc gtacaaactc actgacttac gcccgagacg
     2041 gtgacagcta tcttgtcgtt gcatccaaag gtggcgatcc aaaggctccg ggctggtatc
     2101 acaacgccaa ggcgaatccg ggagttgaaa tcaacgtagg acccaagcgt ctgccagtca
     2161 cggcgcggcc ggtgctaccg gatgacacgt catacgaccg cttgtgggag atcgtcaaca
     2221 agaacaactc gaatcggtac aagaactacc agactcggac gtcccgcccg attcccgtcg
     2281 tcgttctaac gccgagagtc taaaagaagg cccgagcccc caactacgcg acggtgctgc
     2341 cctggcctta gtggtcgtcc aagcgacgac aaacacacca atttcgcagg tctgggattc
     2401 gttgggggca ttcgttgata cgtgattggc cgtcgcccat cagcggcaag atcatctcca
     2461 taatgaccaa tgcgggaaga taaccgccgg aggagccgtg accacaccat caccaccgcc
     2521 cggttggcat ccagacccca gcggagcgcc gggcagcgct acttcgacgg cacccagtgg
     2581 acagaacacc gatccggggc atccgctcgt ttcgggctat ccagcgagga gtgtgcgggc
     2641 aggctggatg ccacaatcgc tgagtgcgtc cgctatggcg atgaagtcgg tgaccgaggc
     2701 catcgtcaag ggtgacccga acggctggcg ggcggtggcc gagatggccg agaacaaaag
     2761 cggccgaact acttccgcgc tctggtttgc gctccaagat cgacgggtag gcggaccacg
     2821 tcacggcaac aacaagaagg aaggtgacgc atgagcacaa ccagcgaaac cggccatgtg
     2881 acgggcacca aggacaag
//
LOCUS       DASYLX010000913         8545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_823477,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000913 DASYLX010000000
VERSION     DASYLX010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823477"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VGP27_21320"
     CDS             complement(<1..107)
                     /locus_tag="VGP27_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7853873.1"
                     /translation="MKSVLLSRRDLDFLLFEWLRVHELTNRERFAEHSR"
     gene            complement(164..553)
                     /locus_tag="VGP27_21325"
     CDS             complement(164..553)
                     /locus_tag="VGP27_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853874.1"
                     /translation="MKEAAAQRYAEVQSLERQRAQVTEQARALSDFIDGMNRLGIRAR
                     RQPFQVLKGSPAENRYRASRLHTTVGWDIGASAVVTPSGSVYDMRNQDKEPCDLTRPQ
                     MFSMTSDRRQSLTELLREALRRAMADC"
     gene            811..1104
                     /locus_tag="VGP27_21330"
     CDS             811..1104
                     /locus_tag="VGP27_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEV7853875.1"
                     /translation="MAAEWVPSQISPDPQGGRMLDTALGILIGLRRCRSEAAFQELLV
                     AAQRHRVPIAAMAWGLVHLTCGGDRSPETVNAAQSAARHEWGQLLAQPAVLPC"
     gene            1137..1796
                     /locus_tag="VGP27_21335"
     CDS             1137..1796
                     /locus_tag="VGP27_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7853876.1"
                     /translation="MAAQLGCALLSLPLTAPAAADPATSSAGQPAQWIVVGVPTANAT
                     TGTLIAFQRVGQGWKPVLGPTAAEVGELGVGAPADGVHRTPVGTFAFDQAFGRQPNPG
                     TKMPYFQATAEDWWDEEADSPTYNTHVRGSGSPSSIAENLHDSGPVYDYAVNIAVNPQ
                     RIPGRLSGIFLHVTDGSPTWGCVAIGREEMRSVLNWLDPSASPRITIGVGSPSLAAMG
                     S"
     gene            2023..3690
                     /gene="sulP"
                     /locus_tag="VGP27_21340"
     CDS             2023..3690
                     /gene="sulP"
                     /locus_tag="VGP27_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEV7853877.1"
                     /translation="MPGVAQFRGYERGWLREDVIAGLTVTAYLVPQVMAYSTLAGLPA
                     VTGLWAAIVALTVYVLIGTSRQLSMGPESTTALMTAAVLAPLAVGDSVRYGMLAATLA
                     VLVGAVCFVGAVARLGFLANMLSRPVLVGYMTGIAILMIASQLGKITGAPVSGDEFVG
                     LMRSFAQNIQQCHWPTMALAASVLILLLALGRWAPRMPGPLIAVLAATVGVFALSLQA
                     KGIEVVGAIPTEWPATDLSRVAPSDVVALILPAVGIAIVAFSDNVLTARAFARGDGDE
                     IDASAELRALGAANVGVGLAHGFPVSSSGSRTAIGDAVGSRTQLYSMVAAVCVSAVVL
                     WGSSVLARFPVAALGALVVYAATRLINLAEYRRLARFRRSELILALATAAAVLGVGVL
                     YGVLAAVALSILDLLRRVAHAHDSVLGFVPGLAGMHDIEDYPAAQSVPGLVVYRYDAP
                     LCFANAQDFRRRAMAAVEQDHARVEWFVLNAEANVEVDLTALDALGQRRDELSRKGIT
                     FTMARVKQDLRDALAAAGMLTKIGEDRIFMTLPTAVEAFEKRQGPRS"
     gene            3662..4177
                     /locus_tag="VGP27_21345"
     CDS             3662..4177
                     /locus_tag="VGP27_21345"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV7853878.1"
                     /translation="MRSVKVLGARSALPQSSIRDAADAAVALEPLAGGAASTLFAVGL
                     IGAAFLAGSLLPLSTAYSVCEYAGVEAAVDDRFSEARLFYVTFGLVTVGGAIAVLIPN
                     APLVTILVATQVLNAVLLVPLLIVMVNIGRDRDLISRFTIRLRGTIAHWTTTAVVVGC
                     VAALAITAVTG"
     gene            4321..5325
                     /locus_tag="VGP27_21350"
     CDS             4321..5325
                     /locus_tag="VGP27_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7853879.1"
                     /translation="MLSPIAWRTPPRHYGPWEQFASLLTEGLVAAGHRVTLFATADSI
                     TEASLHATAPCGWSEDATIDAKVAECLHVASVFEHAGDFDIIHNGFDFLPLTYSGLVT
                     TPVVTTIHGFSSDRIVPVYRRYDSACAYVSISDSDRHPDLHYAATIHHGIDIDGFTVD
                     PNHGEHLLFFGRIHPDKGTAHAIEVARRCGRRLDIAGIIQDEDYFRNRIAPHVDGKKV
                     RYLGAVDASARAEVLAGAHALLHLIDFDEPFGYSVVEAMACGTPVIANARGSMGELIE
                     HEVTGFLVDDIESAVAAVAAAGDLDRRKIARLAAQRFTVTDMIDKYVHVYREVIGNRN
                     "
     gene            5322..7013
                     /locus_tag="VGP27_21355"
     CDS             5322..7013
                     /locus_tag="VGP27_21355"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV7853880.1"
                     /translation="MTPPQEVWWKSAVVYQIYPRSFADSNGDGVGDLGGIVGRLDYLR
                     ALGVDVIWLSPIYRSPQADNGYDVSDYRDIDPLFGTLVDFDKLLAEVHERGMKLVMDL
                     VVNHTSDEHPWFVESRSSRGGPKRDWYVWRDARDGGEPNNWGSFFGGSAWAWDRTTEQ
                     YYLHLFDRKQPDLNWENPQVREAVHEVMRWWLDRGVDGFRMDVINFISKTAGLPDAAA
                     IPGQRFVSAMDEFVDGPHVHAYLAEMTREVFTGRDGEFITIGEMPGVTIAHARLYTDP
                     ARSELDMVFQFEHVSVDQGPRGKFDYIGLDLVMLKRSLHRWQTALAETGWNSLYWNNH
                     DQPRVVSRFGDDHPDYRATSAKALATVLHGMRGTPFVYQGEELGMTNYPFRVPQDHKD
                     LEAINYYKSVLELGLDEGEALAGLAKISRDNARTPMQWDAGPNAGFTTGDPWQYVNPN
                     YTWLNAAAQIGRTDSVFAHYQALIRLRHELPILVDGDFAPLMAEDPQVWAFTRTTPSR
                     RLLVIANCGRNPMTVEIGREWIAADLLLGNLPETPAKSMSSTLDLAGWDARIYSL"
     gene            7080..7565
                     /locus_tag="VGP27_21360"
     CDS             7080..7565
                     /locus_tag="VGP27_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEV7853881.1"
                     /translation="MAERDRDECGRPQSDRPRDALGRPLPRGSEGIPRIPDYLDLPLA
                     ETLALAQNLLDRGLAFNAHEVLEAAWKNGPDDERTLWQALAQLAVGITHVQRGNGKGA
                     VTVLRRASAGLALDGSAPYDIDVSGLADYVDVLIDYLAANAAITPERLTPRLVVRPLR
                     R"
     gene            8124..8312
                     /locus_tag="VGP27_21365"
                     /pseudo
     CDS             8124..8312
                     /locus_tag="VGP27_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1485 a         2721 c         2695 g         1644 t
ORIGIN      
        1 tctcgcgagt gctctgcgaa gcgctcccgg ttcgtcaact cgtgaacgcg taaccactcg
       61 aacagcagaa aatccaagtc gcgccgcgac agcaacaccg atttcataac cgtcctctgc
      121 tcatcacaga cggggacatt cgctatgact ctgccgcatc gacctagcag tccgccattg
      181 cgcgcctcag tgcttcgcgg agcagttcgg tgagggattg ccggcgatcg cttgtcatcg
      241 agaacatttg aggtcgtgtc agatcgcagg gctccttgtc ctggttcctc atgtcgtata
      301 ccgagccact gggtgtgacg accgcgcttg ctccgatgtc ccaaccaacc gttgtgtgta
      361 agcgcgacgc gcggtagcgg ttctcggccg gagatccttt caacacctgg aagggctgac
      421 gccgcgcccg gattcccagt cgattcatgc cgtcgatgaa atcgctgaga gcacgcgcct
      481 gctcggttac ttgggctcgc tgacgctcaa gcgattgcac ttcggcgtag cgctgtgccg
      541 cggcctcctt caacgcctct tcccacacgc ccatcgttgc ttaccatcca tgcttctagt
      601 acgaccctga cgcggggtta ccactgccgg agccgtctga atcggacggg aatgtggatg
      661 tgatggccac ggtgccgtcg cagggcggtc acggctgcat accgatgcgc tccggtctct
      721 ggtttcaacc gtcggctggc acaaaatgga ctcttggtca ggctgccgat ctcgcgcgtc
      781 ggtgcggtcg tcagagcaga taggcaaggt atggcagcag agtgggttcc ctcccaaatc
      841 tcacccgatc cgcagggcgg gcgcatgctc gacaccgcgc tgggaatatt gatcggactt
      901 cggcggtgtc gttcggaagc agcgtttcaa gagctgctcg ttgccgccca gcggcaccgg
      961 gtccccatcg ccgccatggc gtggggcttg gttcatctga cctgcggagg cgacaggtca
     1021 ccggaaaccg tcaacgccgc gcaatcggcc gcgcgtcatg aatggggcca gctgcttgcc
     1081 cagcctgcgg tgttgccctg ctgagagcgg tccgcgcatc gaaactctcg acgttggtgg
     1141 cggcacaact cgggtgcgcg ctgttgagcc tgccgctcac cgcgccggcg gcggcggacc
     1201 ctgcaacgtc ctccgccggt cagcccgcgc agtggatcgt cgtgggcgtg ccgactgcga
     1261 acgcgactac aggaaccctc atcgcctttc agcgggtcgg tcaaggatgg aagcccgtac
     1321 tgggaccgac ggccgccgaa gtaggcgagc tcggcgtcgg cgcccccgcc gatggcgtcc
     1381 accggactcc ggtgggaacg ttcgcgttcg accaagcctt cggccgccaa cccaacccgg
     1441 gcaccaaaat gccctacttc caggccactg ccgaagactg gtgggacgag gaggccgact
     1501 cacctactta caacacgcac gtgcggggat ccggtagtcc ctcgtcaatc gcggaaaacc
     1561 tccacgactc cggtcccgtc tacgactacg ccgtcaacat cgcggtaaac ccgcaacgca
     1621 tcccgggacg gctgtcgggg attttcctcc acgtaaccga cggcagtcca acctgggggt
     1681 gtgtggctat cggccgcgaa gagatgcgtt ccgtgctgaa ctggctcgac ccttccgcct
     1741 ccccgcgaat caccatcggc gtcggtagcc catcgctggc cgcaatggga tcgtgagccg
     1801 gcttgcggcg acacgatcaa cggcgctctc cgcgtcgagc gactcaccga gacaccgacc
     1861 gcttcatcgg ttgacgccgg acatgaaatc catcttcctc cggcaagttt ttggaatgct
     1921 gtatcagtag gccggccgcg aacactgggc aggctcagcg tggccgcggc aaggtcttag
     1981 gaggctcgtc gatgatcgcg accaggagcc ttcgcgactg gggtgccggg ggtcgcacaa
     2041 ttccgtggtt atgaacgcgg gtggcttcga gaagatgtca tcgcgggact cacggtgacg
     2101 gcctacctcg tgccccaggt gatggcgtac tcgacgttgg cggggctgcc cgctgtcacc
     2161 ggtttgtggg cggccatcgt cgctctgacc gtgtatgtcc tgatcggcac gtcgcggcag
     2221 ctgtcgatgg ggccggaatc gacgaccgcg ttgatgacgg ctgccgtgct cgccccccta
     2281 gcggttggcg acagtgtgcg ctacggcatg ctggcggcca ctctggcggt gttggtcggt
     2341 gcagtgtgct tcgtcggcgc ggtcgcgcgg ttggggttct tggccaacat gctgtctcgt
     2401 ccggtgttgg tgggctatat gacaggcatc gcgatcctca tgatcgcaag ccagctgggc
     2461 aagatcaccg gcgcaccggt ttcgggcgat gaattcgtcg gtctgatgcg atccttcgcc
     2521 caaaatatcc aacaatgcca ctggccgaca atggccttgg cggcatcggt cctgattctg
     2581 ctgctcgcgc ttggacgctg ggcaccgaga atgccgggcc cgttgattgc cgtgctcgcg
     2641 gcgaccgtgg gtgtgttcgc gctgtcattg caggccaagg gaattgaagt cgttggtgcc
     2701 atccctaccg agtggcccgc gaccgacttg tcgcgcgtgg cgcccagcga tgtggtggcg
     2761 ctgatcctgc cggcggtcgg aatcgctatc gtcgcgttct cggacaacgt gctgactgcg
     2821 cgcgccttcg cgagaggtga cggcgatgaa atcgacgcga gcgcggagct gcgcgcactc
     2881 ggtgccgcca acgtaggtgt tggcctggct cacgggtttc cggtcagctc cagcggatct
     2941 cggactgcga taggggacgc cgtcggcagc cgcacccagt tgtactcgat ggtggcggcc
     3001 gtgtgcgtat cagccgtcgt gctatggggg agtagtgtgc tggcgcgctt tccggtcgca
     3061 gcgctaggcg ccttggtcgt ttatgccgca acgagattga tcaacctcgc cgagtaccgg
     3121 cgccttgccc gatttcgccg cagtgagctg atcttggcgt tggccaccgc cgctgccgtg
     3181 ctgggcgtcg gtgtgctcta tggggtgctg gcagcggtcg cactgtcgat acttgatctg
     3241 ctacgacgcg tcgcgcacgc gcatgacagc gttctgggtt tcgttccggg gctcgccggc
     3301 atgcatgaca tcgaggacta tcctgccgcg cagtcggtgc cgggacttgt cgtgtaccga
     3361 tatgacgcgc cgctgtgttt cgcgaatgca caggacttcc gccgtcgtgc aatggccgcc
     3421 gtggaacagg accatgctcg agtggagtgg ttcgtgctca acgccgaggc gaacgtggaa
     3481 gtcgatctga ctgcgttgga cgccctgggt cagcgtcgag acgagttgtc gcgtaaggga
     3541 attacgttca caatggccag agtcaaacag gacttgcgag acgccctcgc cgcggccgga
     3601 atgctgacca agatagggga ggaccggatc ttcatgacct tgcccactgc ggtcgaggcg
     3661 tttgagaagc gtcaaggtcc tcggagctag gagtgctctc cctcagtcat ccattcggga
     3721 tgccgccgac gccgcggtcg ccctcgaacc tcttgccggc ggcgctgcgt cgacgctgtt
     3781 tgccgtcggc ctcatcggcg cagcgttcct ggcaggctcg ttgctgcccc tgtcgactgc
     3841 gtactcggtg tgcgaatatg ccggcgtcga agcagcggtc gatgaccgct tcagcgaagc
     3901 gcggctcttc tatgtgacat tcgggctcgt gacagtcggc ggcgcaatcg ccgtgctcat
     3961 cccgaatgcg ccgctggtga cgatcctcgt ggctactcag gtgctcaatg cggtgctgtt
     4021 ggtaccactg ttgatagtga tggtcaatat cggacgagac cgtgatctga taagccgctt
     4081 caccatccgc ttgcgcggaa cgatcgccca ctggacgaca accgccgtag tggtcgggtg
     4141 cgtcgccgca ctggcgatca cagcggtcac gggctgagca agcgccgagc attcgtgagt
     4201 ccgggcgagg cgatgggcat ccggcccgcc catgaccaac aagccggtag cgccggtgtc
     4261 ggcgggtagc ctgagcacat tcccattcac agcgagactg gcggtcccat ccgagtcgct
     4321 gtgctgtcgc cgatcgcgtg gaggacaccg cctcgtcact acgggccgtg ggagcaattc
     4381 gcctcgttgc tcaccgaggg attggtggca gcaggccatc gtgtcaccct gttcgcgacc
     4441 gcagactcga tcaccgaggc cagcctgcac gcaaccgcgc cgtgtggttg gtctgaagat
     4501 gcgacgatcg atgccaaggt cgcagagtgc ctgcacgtcg cctcggtgtt cgagcatgcc
     4561 ggcgacttcg acatcattca caacgggttc gacttcttgc cgctcaccta cagcggcctc
     4621 gtcacgacgc cggtggtcac cacaatccac gggttctcgt ccgatcgaat cgttcctgtt
     4681 tacaggcgct acgacagcgc atgcgcgtac gtgtccataa gcgattctga tcgacacccg
     4741 gatctgcact acgccgccac aatccaccac ggcatcgata tcgacggctt caccgtcgat
     4801 cccaatcacg gcgagcatct gctgttcttc ggacggatcc atcccgacaa agggaccgcc
     4861 cacgcgatcg aggtcgccag gcgatgtggc cgtcggctcg acatcgccgg gatcatccaa
     4921 gatgaggatt acttccgcaa caggatcgcg ccgcacgttg acggcaagaa ggtgcgttac
     4981 ctcggggctg tcgatgcctc tgctcgtgcc gaggtactcg cgggcgcgca tgcgctgctc
     5041 cacctgatcg acttcgacga accgttcggt tacagcgtcg tcgaggcaat ggcctgtggc
     5101 acaccggtta tcgccaacgc gcgaggctcg atgggcgagc tgatcgagca cgaggtgacc
     5161 ggcttcctcg tcgacgacat cgagtcggcg gtggcagcgg tggccgccgc cggcgacctc
     5221 gaccggcgca agatcgccag gctcgccgcc cagcgcttca cggtcacgga catgatcgac
     5281 aagtacgtgc acgtgtaccg agaagtcatc ggcaaccgga attgacaccg ccccaggagg
     5341 tgtggtggaa gtcggccgtc gtctaccaga tctatccgcg gagctttgcc gactcgaacg
     5401 gcgacggcgt cggcgatcta ggtggcatcg tcgggcggct ggactatctg cgtgcgctcg
     5461 gggttgacgt gatctggttg tcaccgatct accggtcgcc gcaggccgac aacggctacg
     5521 acgtcagcga ctaccgcgac atcgatccgc tgttcggaac cctcgttgac ttcgacaagc
     5581 tgctcgccga ggtgcacgaa cgcggcatga agctggtgat ggacctggtc gtcaaccaca
     5641 cctcggatga gcacccgtgg ttcgtcgagt cgcgttcatc gcgcggcggt ccgaagcggg
     5701 attggtacgt ctggcgcgac gcccgtgacg gtggggagcc gaacaactgg ggctccttct
     5761 tcggcggatc ggcgtgggcg tgggaccgga ccaccgagca gtactacctg cacctgttcg
     5821 accgtaagca accggacctg aactgggaga acccacaggt tcgagaggcg gtccacgaag
     5881 tcatgcgctg gtggctcgat cgcggtgtcg acggctttcg catggatgtc atcaatttca
     5941 tctccaagac tgccggactg cccgacgcag cggcgatccc ggggcagcgt ttcgtcagtg
     6001 ccatggacga gttcgtcgat gggccgcacg tgcatgccta cctcgccgag atgacacgcg
     6061 aggtcttcac cggccgcgac ggcgagttca tcacgatcgg cgaaatgccg ggcgtcacaa
     6121 tcgcgcacgc gcggctgtac accgatccgg ctcgaagtga gttggacatg gttttccagt
     6181 tcgagcacgt gtccgtcgac cagggcccac gggggaagtt cgactacatc ggtctcgacc
     6241 tggtcatgct gaagaggtcg ttacaccggt ggcagactgc cttggccgaa accggatgga
     6301 acagcctgta ttggaacaac cacgaccagc ctcgcgtcgt gtcccgattc ggcgacgacc
     6361 atccggatta ccgggccacc tcggcgaagg cgctcgcgac cgttctgcac ggcatgcgcg
     6421 gcacgccgtt cgtgtaccaa ggtgaagaac tcggaatgac caactacccg ttccgtgttc
     6481 cgcaggacca caaggacctt gaggcgatca actactacaa gagcgtgctc gaactcggac
     6541 ttgacgaagg ggaggcgctg gccggtctgg ccaagatcag ccgcgacaat gcgcggacac
     6601 caatgcaatg ggacgctgga cccaacgccg gattcacgac cggtgatcca tggcagtacg
     6661 tcaaccccaa ttacacgtgg ttgaacgccg cggcccagat cggccggact gattcggtgt
     6721 tcgcgcacta ccaagcactc attcgcctcc gccacgaatt acccatcttg gtcgacggtg
     6781 acttcgcgcc gctgatggcc gaagacccgc aggtctgggc tttcacgaga accaccccga
     6841 gcaggcgact ccttgtcatc gccaattgcg gacgtaatcc catgaccgtc gaaattggcc
     6901 gagaatggat cgcggccgat ctgctactcg gcaacctgcc cgagactccg gcgaagtcga
     6961 tgtcatcgac cctcgacctg gccggctggg acgcacgcat ctattctctg tgattcccat
     7021 actgaatcgg caggtctccc ggttgggaag cgaatctgca ccgatgccat gatgagcacg
     7081 tggctgagcg tgatcgcgac gaatgcggcc ggccccaaag cgatcgtccc cgcgacgcac
     7141 tcggtcgacc gcttccgcgg ggcagcgaag gcattccacg cattccggat tacctcgatt
     7201 tgccgctggc cgaaaccctg gcccttgccc aaaatctgct ggaccggggc ctggcgttca
     7261 acgcgcacga agtgctcgag gccgcatgga agaacggccc ggacgacgag aggacattgt
     7321 ggcaagcact tgcccagctc gcggtcggca tcacccatgt ccagcgcggc aacgggaagg
     7381 gagcggtcac cgtccttcgc cgcgcgtcgg ccggtcttgc cctcgacggc tcggcgccgt
     7441 acgacatcga cgtctcaggt ttggccgact acgtcgacgt gctgatcgac tatctcgctg
     7501 caaacgccgc tatcacgccg gaacgcctga ctccccgtct ggtcgtccga cccctccgac
     7561 ggtgatgaga ttttcgatgg tcacgtacag cttgacgcgt attgtcatgg cttcgacctg
     7621 gtagcggcaa agccgctccg gcgagatcgc ttgtccagca ggcgagttcc agccgacacc
     7681 tagtcgggat gaactcttgc cttcatctcg cgaataagtt tctctacgct cttccgatga
     7741 tttgcgtacg agcccagttc ccgtgacggg cctagcggat gctctattgc gtacaaaatg
     7801 ctgtctagac gactattcat tattttcctg cttcagtagg tgcctaccat agcacgataa
     7861 gccgctgagc tgcgcttatc tggttctggc ccggccgatg gcgacccggc cgcgagatcg
     7921 ccccaggccg atattcgcca accgcagccg ttatggcggc agaggttcga gtcgcaccgc
     7981 gcagtaggtg tcgatgccac gggcggcccc aagcggggaa agatcttcgt agtgacgcat
     8041 cccatcgcgg cgatgcgatg tcaaacgtcg cattgcgcgt agcccctgca ccgcccgaac
     8101 gagcgcaaag cctcttcgca cggcacccgc aacgcatccc gggacgggcg tcggggattt
     8161 tcctccacgt ggccgacggc agtcccacct gggggtgtgt ggctatcggc cgcgaagaga
     8221 tgcgttccgt gctgaactgg ctcgaccctt ccgcctcccc gcgaatcacc atcggcgtcg
     8281 gtagcccatc gctgggccca tcggggtcgt gacctggctt gcggcgacac accggccgct
     8341 tcaccgagat gcgggccttc atttgcgccg actgtgtggt tttagcgcgc gacgcgccgt
     8401 acgccgcgta atccgcacac tcggtagccg gccggttagc ggcgcacagg ccatggcgtc
     8461 acgttcggca gcggccatct cgtcggcgcg accatcgcaa cggcatgggt gcggcaaaca
     8521 cgcataattg tcgctctaga cagtt
//
LOCUS       DASYLX010000914         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_383804,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000914 DASYLX010000000
VERSION     DASYLX010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383804"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGP27_21370"
     CDS             complement(<1..418)
                     /locus_tag="VGP27_21370"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEV7853882.1"
                     /translation="MTAPDDTPLWLFADQLGPATHGGEHAHRELLLIEAGSALRKRPY
                     HRQKLHLVLSALRHADRDFGDRATLLQTDTYTDALEQYGRRVLVYEPTSHAAERFVYR
                     LRDQGLVAEVLPTPTFALPRKDFTEWAGDRTRFRMED"
     gene            507..583
                     /locus_tag="VGP27_21375"
     tRNA            507..583
                     /locus_tag="VGP27_21375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:541..543,aa:Pro,seq:ggg)
     gene            complement(652..927)
                     /locus_tag="VGP27_21380"
     CDS             complement(652..927)
                     /locus_tag="VGP27_21380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7853883.1"
                     /translation="MFATEVGGWVDPRNLLRTVEIAADKAGIDDVGAHTLRHSAAVGW
                     LESGVHIKAAADLLGHASISITGDLYGHTSDDAARAAVNGLGDALGL"
     gene            961..1446
                     /locus_tag="VGP27_21385"
     CDS             961..1446
                     /locus_tag="VGP27_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853884.1"
                     /translation="MMLGETWQDQYRRMKRSYALVKSDASQKDIGLDALYHFCYDVLH
                     FRDWIKKSDLIDEQARSEVSLLISVQGRKGTSWAIQACADVANAAKHFELRRRDDPPA
                     EVVGHNRKSTLPFTFPATLGPAHFVIDIDGTRYPATTVADAAVSEWNDWLTSRGLDIP
                     E"
     gene            complement(1467..1676)
                     /locus_tag="VGP27_21390"
                     /pseudo
     CDS             complement(1467..1676)
                     /locus_tag="VGP27_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2310..2651)
                     /locus_tag="VGP27_21395"
     CDS             complement(2310..2651)
                     /locus_tag="VGP27_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEV7853885.1"
                     /translation="MTITIDVPLADPEAAALDLIVQAAETASGSTAFRAVLQDLAGAL
                     EGAFVGLVWDDARLAAAAIAYDACTEGDAVGSLRIRSASMRAGYWDRHCANPEGIAQA
                     LDVAATVLDVM"
BASE COUNT          666 a         1145 c         1182 g          652 t
ORIGIN      
        1 agtcctccat gcggaagcgc gtgcgatcac ctgcccattc ggtgaagtcc ttgcggggaa
       61 gcgcgaaggt cggtgtgggc agaacctcgg cgacgagtcc ctggtcgcgc agccggtaga
      121 cgaaccgctc ggcggcgtgc gaggtcggct cgtagacgag gacccgacgg ccgtactgct
      181 cgagcgcgtc ggtgtaggtg tcggtctgca gcagtgtggc gcggtcaccg aagtcgcggt
      241 cggcgtggcg cagcgccgag agcacgagat gaagcttctg gcggtggtag ggacgcttcc
      301 gcaacgccga gccggcctcg atgagcagca gctctcggtg agcgtgctca ccgccgtggg
      361 tggcggggcc gagttggtcg gcgaagagcc acagcggggt gtcgtcaggg gcggtcacga
      421 cacgggtgta cccgctggct gcggttggga ttgtgacgcc gcgctgcggt aggctcacgt
      481 gctgctgccg atcaaatcgg gggcaccggg ctgtggcgca gtttggtagc gcacttgact
      541 gggggtcaag tggtcgcagg ttcaaatcct gtcagcccga ccaaggtcag aggcggtttc
      601 cgaggtttgg aagccgcctt tttcatgccg taccgccttt gcataccgcc atcacaatcc
      661 gagcgcatcg ccgagtccgt tgactgcggc ccgcgcggcg tcgtcgctgg tgtgcccgta
      721 aaggtcgccg gtgatgctga tcgacgcgtg gccgagtagg tcggccgcgg ccttgatgtg
      781 cacgcctgat tccagccagc cgacggctgc cgaatggcgc agcgtatgcg cgccgacgtc
      841 gtcaatgccg gccttgtccg ccgcaatctc gacggtgcgc agcaggttgc gcgggtcgac
      901 ccagccgccg acctcggtgg cgaacactgc gcccgacgca ccgatcggtg agaaggtcga
      961 atgatgctcg gggaaacatg gcaagaccag taccgcagga tgaaacgctc gtacgcgctc
     1021 gtcaagagtg atgcgagcca gaaagacatc ggactcgatg cgctctacca cttctgttac
     1081 gacgtgctgc atttccgcga ctggatcaag aagtctgacc tgatcgacga gcaagccagg
     1141 agcgaagtgt cgcttttgat cagcgtccaa gggcgcaagg gcacgtcatg ggcaatccag
     1201 gcctgcgctg acgtcgcgaa cgcggcgaaa cactttgaac tgcggcgtcg tgacgaccca
     1261 cccgcggaag tcgttggcca caatcgaaaa tcgacgttgc ccttcacgtt ccccgcgact
     1321 ttgggaccgg cccattttgt gatcgacatc gacgggacgc ggtaccccgc cacaacggtc
     1381 gcagacgcag ccgtgtcgga atggaacgac tggctgacca gccgcgggct cgacattccg
     1441 gaatgagact ccccgcggag cgcgggctat accgttcccg ctgacatcat cgcagcccgt
     1501 atctcggcgt cgtcaatggc cgtgtagcgc tccgttgtcg cgacgctgga atgcccgagc
     1561 agcacttgca ccgcgcgcag ctgccgggat ccgcggtacg cccgggtcgt gaatcggtgg
     1621 cgcaaggtgt gcgccgtcca gtgatcgggc agcgcgccgg ccatcagctt cccgactcac
     1681 cgcggcaggc acctgaactc gtggtgcaac ggcatcacat cgcaccgcaa caggcgccgg
     1741 ccccgcgtcg atcaggtcaa cggtttcgca ttccgaagcg ccgccagcac tcgatccctc
     1801 gtcggaaggt ccaatcggca cgcatcagct gtcaattcag tgccccgctt catgacatca
     1861 acaccatcaa cgagcacggt cggcgatgga tattcaccga ccctttcaac caccacatct
     1921 tcagcgatat caacctcaga cagacagtcc ccgagcaagc gtcgagccgc cgccgcattc
     1981 ggacacgccc ggctcgtaag aagttccaca cgcatatacc gagcttcgct caatacgcac
     2041 ggctgccgca attaccaagt ggggccaatt gacaccgccc atgcgggagc caggtcaagt
     2101 tgacatagcc acctgctggc cgaaccacgt aagaatgccc gccgttgcga ccggcgctgg
     2161 tccgcacttc atcccgcgcg ccatgtgggc caggtggctt cggcgagtgg cgttcgtcgc
     2221 cggcgaccgg cccgcgccgg cttcggccgc gaggtacgcc gcaatcaggt cggcccatgc
     2281 cgacggaagc ggttgtggcg cgggcccgct cacatcacgt ccagcaccgt ggcggcgacg
     2341 tcgagcgcct gggcgatgcc ctcggggttt gcgcaatgcc ggtcccagta gccggcgcgc
     2401 atcgaagcgg agcggattcg cagcgagccg acggcatcgc cttccgtgca tgcgtcgtag
     2461 gcgattgccg cggcggcgag tcgagcgtca tcccatacca ggccgacgaa cgcgccttcg
     2521 agggcgccgg cgaggtcttg cagcacggcg cggaatgcgg tactgccgct ggcggtttcc
     2581 gcggcctgga cgatgaggtc gagtgcggcg gcttcgggat cggccagcgg aacgtcaatc
     2641 gtgatcgtca tgcaccgaag ctaacccaat ggatatcgaa ttgccaaggg ctgcaaagaa
     2701 agtcgtacat atgtcgcacc agcgcaaacg ccgcatagcg acatatgtcg gaatctgcac
     2761 agatgccgcc ggtggacggc gacctatgcg acactttggc gcgtggacgg ctttcttcta
     2821 ctgatggcga gcgtcggccc atggcagtta gcgggtgtgc tcgctgacct gggcctactt
     2881 ctgctagccg gaacattgtg ggcgtggaga cgacctacgg cacgttcact acgccaagga
     2941 aaaacggacc ccaagccgtg aacggactca ccttcgatcc cggtggatat gggccacatc
     3001 cacgccacga gcgatcgctc cgagcgtcct gccggccatt gtttccgggc tgcgggactg
     3061 tcggtccgta ccgccatccg taccgccatt aagtcgattt gaccgctgtt ttccgtgtcc
     3121 gtccgtagct gtgtctgtgt gcgggaatcc gttgtgctac agggtctata ggacacttct
     3181 acggatgcct gcggataccg ccgttatgcc tgggggtcta gtggtcgcag gttcaaatcc
     3241 tgtcagcccg accagaaaat acgcagttca caggcttcgg aggaatccgg agccgcctcg
     3301 attgcgtcag aagcgtcgtc gatcgccaac aaaccccgtg tgccgagatc ctatggtgca
     3361 ggcatgtttt cgcgggcctc cttgttcgcg tccgaatatg actgaatcga gtacggaggg
     3421 ttcccgctac ttccgccggt ctcaagatct ctgcgttgac cagcgccgac agacgctggg
     3481 tgagcgcgac gtcagtcgtt cgggagaaat cggccagcgc cgttcgcata cggcgttcgc
     3541 cgtaggacgg tcggcagctc ccgggctgat cggcggggcc agcacggaaa gtctaaactt
     3601 atagggctcg gtaaatagac tgtgcagcaa catattccgc ggcgg
//
LOCUS       DASYLX010000915         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3128833,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000915 DASYLX010000000
VERSION     DASYLX010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128833"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1237)
                     /locus_tag="VGP27_21400"
     CDS             complement(119..1237)
                     /locus_tag="VGP27_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEV7853886.1"
                     /translation="MDELHNNYGPEARYAVEAEAALPTTKWQQEITRGLELGLQGADS
                     IVDRRIPTFSRGELPHFAGINTFLKAPYVEDVRRCGEYDVAVLGAPFDGGTTYRSGTR
                     FGPQGIRKISALYGPYSFELGVDLRESITIADLGDVFTIPGNIEKTFDQISKAVGHVF
                     SSGAFPVVLGGDHSIGYPTTRAVAEHLDGGKLGIIHFDRHVDTQETDLDERMHTTPWF
                     HATDIPNVPPQNLVQIGIGGWQAPRPGVQVGRERGTTIMTVTDCVEMGIEEAAQRALD
                     VAWDGTDAVWLSFDVDCLDAAFVPGTGWPEPGGFLPREVLKFIQLVAARPLAGIEVVE
                     CSPPYDNAEITALIATRVICDTLGCLVRAGHLPRKAAS"
     regulatory      complement(1290..1394)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
BASE COUNT          352 a          596 c          652 g          361 t
ORIGIN      
        1 cccgcggcga aggaggagaa ggcgccgagc ctgcggtgca gctcctgccg gtagccgatg
       61 ctgcgcagat cgtcggcgtc cgggccgccg gtcgagctcg tgaggagagt cggagtattc
      121 atgaagccgc ctttcgtggc aggtgaccgg cgcgtaccaa acagccgagg gtgtcgcaga
      181 tgacccgggt cgcgatgagc gccgtgattt cggcgttgtc gtagggcggg gagcactcca
      241 cgacttcgat tcccgccagc ggacgcgcgg cgacgagctg gatgaacttc agcacctcgc
      301 ggggtaggaa gccgccaggc tcgggccatc ccgtacctgg gacgaatgcg gcgtcgaggc
      361 agtcgacatc gaaggacagc cagacggcgt cagtgccgtc ccatgcgacg tccagggcac
      421 gctgcgcggc ttcctcgatg cccatctcga cgcagtcggt gacggtcatg atcgtcgtgc
      481 cgcgctcccg gccgacttgc accccgggcc gtggcgcctg ccaaccgcct ataccgatct
      541 gcaccagatt ctgcggcggc acgttgggga tgtccgtggc gtgaaaccat ggggtggtgt
      601 gcatgcgctc gtcgaggtcg gtttcctggg tgtcgacgtg gcgatcgaag tggatgatgc
      661 cgagcttgcc gccatctagg tgttccgcga ctgcacgggt ggtcgggtag ccgatggagt
      721 ggtcgccacc caatacgacc ggaaaggcgc cggaggagaa gacgtgcccg accgctttcg
      781 agatctggtc gaaggtcttc tcgatgttgc ccggaatggt gaaaacgtcg ccaagatcgg
      841 caatggtaat ggattcccgc aggtcgacac cgagctcgaa gctgtacggc ccgtatagcg
      901 cggagatctt gcgaatgccc tggggaccga agcgggttcc ggaccgatac gtggtacccc
      961 cgtcgaaggg tgcgccgagc accgccacgt cgtattcgcc gcagcgccgg acgtcttcaa
     1021 cgtaaggagc cttgaggaag gtgttgatgc cggcgaaatg cggcaactcg ccacgagaga
     1081 aggtgggaat ccttcggtcg acgatcgagt cagcgccttg cagacccaat tcaaggcccc
     1141 gggtgatttc ctgctgccat ttcgtcgtcg gcagcgctgc ttccgcctcg acggcgtatc
     1201 gtgcctccgg gccgtagttg ttgtgcagct cgtccacggt gcgcctccgc gtccacggcg
     1261 cccggccgcg aggcagccgg gcgtggctga cctcccgggc ttctatcccg ccgtggagcg
     1321 ggcagtgtct gcccgcctgc acctctcggt ccagactcgc tgcagtcagc gacggaaccc
     1381 taggtgcgcc tcacccacac gagtgagtca caactatcac acggcatggc tatgacgagc
     1441 atgtaaccac tgagttgcgg tcacatcacc tatcagctgg tactgacgct ctccgctcgg
     1501 gcaattggca ccgcaccggg agacaagagg tatctcgctc gtcgcaacat caccgccggc
     1561 agggtagctg cacggctagc ccgactctca gtagatagca gccgtcgtcg gtcaatggtc
     1621 agtccgcaag cgacagacca ggaggggaac atcgtggcct atccaggtga cggtcggtta
     1681 tcggtgatct acgtgcgggg gtcacgggtt caggtatcga cagggtggtt gacgacccgt
     1741 tctacgggtt caacgaggtt cggtccatgt ccgcgtcgat ggcgccggcg agccgcggtt
     1801 gctccagttc gaaagtccct tactccggtt aatcaccgat gagggctatc agctgtaagt
     1861 ccacggcgat caacgtgcat acttggccgg ccaagatgac ggaaatgtcg atgtgaacac
     1921 aattgggtgc accggtttac gacgtgtcgg catccacatt c
//
LOCUS       DASYLX010000916         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1261301,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000916 DASYLX010000000
VERSION     DASYLX010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261301"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2200)
                     /locus_tag="VGP27_21405"
     CDS             complement(<1..>2200)
                     /locus_tag="VGP27_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV7853887.1"
                     /translation="DRIEGFTGELLCLDALSVPLQQDAVARPTATTSAHTAYVIHTSG
                     STGAPKGALNTHGGIRNRLLWMQQADPLSADDRVFQQTPVTFDVSVLEIFWPLIAGAQ
                     LVIAKPDGHKDIDYVVKTIAENSVTAAFFVPTMLRSFLAQPAVTDCAALRRVSCGGEL
                     VPYELTQRFHATLKAELWNEYGPAETAVTATYFHCKRGASGPTVPIGRPIANVRVHLL
                     NAHGRPVPVGAAGEMFIAGAGVGRGYLNQPDATAASFIPDPFAEHPDQLMYRTGDLAR
                     YLPDGNIEYLGRLDDQVKVRGVRIELGEVEAALAKHPDVRENAVVAGKDDRGNTRLVA
                     HVVAAGQPAPTTNELRRFLIEQLPAAMVPSVFCTAATLPRTPGGKVDRRALVAAAEAA
                     PSEAQEFVAPHTDAEKLLAKIWCEVLELERVGVHDDFFALGGCSTHSMEVTVKAEAAG
                     MPLTPEAVFVCSTIADLAAEYGRGADAVVEPAEGLDVVDASDEESPEAEEPQAAPTPA
                     VATPTVGPQTRNTVVESIGTYLPAEIVSTDAVLAGCTVEVGIPLERLTGIKNRRVVGR
                     GEYSIDLARQAMDDCLGRSTYGPEEIDLIIACNISRCDGPDHKFVFEPGTAARLRDRC
                     GLTNAIAFDISNACAGMWTGINVADAYLKTGLVQRAMVVSGEYITHLTETAQKEIEGP
                     MDSRLACLTLGDAGAAVILERGTGDRVGFHDIDMATLGAYSKLCVAKTTDG"
BASE COUNT          367 a          722 c          724 g          387 t
ORIGIN      
        1 ccatcggtcg tcttggccac gcagagcttg ctgtaggcgc cgagggtggc catgtcgatg
       61 tcatggaagc cgacacggtc acctgtgcca cgctcgagaa tcactgccgc cccggcgtcg
      121 ccgagggtca ggcacgcaag acgggagtcc atcggtcctt caatttcctt ctgcgcggtc
      181 tcggtcaggt gagtgatgta ctcgccgctc actaccatgg cgcgttgcac caggccggtt
      241 ttcagatagg cgtcggcgac gttgatgccc gtccacatgc ctgcacacgc gttgctgatg
      301 tcgaatgcga tcgcgttggt cagtccgcac cggtcgcgaa gacgcgccgc cgtgccgggt
      361 tcgaacacga acttgtgatc gggaccgtcg cagcgagaga tgttgcaggc gatgatcaga
      421 tcgatctctt cgggcccgta agtcgagcgg cccaggcagt cgtccatcgc ctgccgggcg
      481 aggtcgattg agtactcgcc tctgccgacc acccgccggt tcttgatgcc ggtcaggcgc
      541 tccaacggga tgccaacctc aacggtgcac ccggcgagga cagcatcggt cgagacgatc
      601 tctgcgggga gataggtccc gatgctctcc accaccgtgt tgcgcgtctg cgggccgacc
      661 gtcggggtcg caacggccgg agtgggtgcg gcttgcggtt cttcggcctc gggcgattcc
      721 tcatcagaag cgtcgacgac atcgagaccc tcagcgggtt cgacgactgc gtcggcaccg
      781 cggccgtact cggctgccag atcggcgatc gtgctgcaca cgaacaccgc ctccggggtg
      841 agcggcatac cggccgcctc ggccttcacg gtcacttcca tgctgtgcgt ggagcaaccg
      901 cccagcgcaa agaaatcgtc gtgcacgcca acacgttcca gctcgaggac ctcacaccag
      961 atcttcgcca gaagcttctc ggcgtccgtg tggggggcga cgaattcctg cgcttcggat
     1021 ggcgcggcct cggcggcagc aaccaatgca cggcgatcca ccttcccacc cggcgtgcga
     1081 ggcagcgtcg ccgccgtgca gaagacggac ggcaccatcg ccgcaggcaa ctgttcgata
     1141 aggaatcgac gcagctcgtt ggtcgtcggt gcgggctgcc ccgccgcgac cacatgggcg
     1201 accagccggg tgtttccgcg gtcgtccttg cctgccacca ccgcgttctc ccgaacgtcg
     1261 gggtgcttgg ccaacgcggc ctcgacctca ccgagctcga tccgcacacc gcggaccttc
     1321 acctggtcat cgagacggcc cagatactcg atgttgccgt ccggcaggta ccgtgccagg
     1381 tcgccggtgc ggtacatcag ctggtctggg tgctcggcga acgggtcggg gatgaagctc
     1441 gcggccgtcg catcaggctg gttgaggtat ccgcgtccga cgcctgcacc ggcgatgaac
     1501 atctcaccgg cagcaccaac cggaacgggc cggccgtggg cgttcagaag atgcacacgc
     1561 acattggcga tcggtcgccc gatcggaacc gtcgggccgg atgcgcctct cttgcaatgg
     1621 aaataggtgg cggtgaccgc cgtctcggcg gggccgtact cgttccacag ctccgccttc
     1681 agggtggcat gaaagcgctg ggtgagttcg tagggcacca gctcgccgcc gcacgacacc
     1741 cggcgcagag ccgcgcagtc cgtgaccgca ggctgggcga ggaaggaccg caacatggtc
     1801 ggcacgaaaa acgctgcggt gactgagttc tccgcgatgg tcttcaccac gtagtcgatg
     1861 tccttgtgtc cgtccggttt tgcgatgaca agctgcgcac cggcgatcaa cggccagaag
     1921 atctccagca ccgaaacgtc gaacgtcacc ggggtctgtt ggaaaacgcg gtcgtcggcg
     1981 ctcagcgggt cggcttgctg catccacaac agccggttgc ggatcccccc gtgcgtgttg
     2041 agcgcgccct tcggcgcacc ggtggacccg gatgtgtgaa tgacgtacgc ggtgtgtgcc
     2101 gacgtcgtgg cggtcggccg cgccaccgcg tcttgctgca gagggaccga cagcgcgtcg
     2161 aggcacaaga gttcgccggt gaatccttcg atgcgatcga
//
LOCUS       DASYLX010000917         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_934018,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000917 DASYLX010000000
VERSION     DASYLX010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934018"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP27_21410"
     CDS             complement(<1..306)
                     /locus_tag="VGP27_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7853888.1"
                     /translation="MTDPVQTPSGIPLQPVYGPGDRAGEPPAPGAYPFTRGNFSGGYR
                     GKLWTFRQYSGFGTAEDSNRRYRYLLDQGGTGLSVALDLPTQCGFDSDDPEVSEEVGR
                     "
     gene            493..1350
                     /locus_tag="VGP27_21415"
     CDS             493..1350
                     /locus_tag="VGP27_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853889.1"
                     /translation="MSRQSLTGRTMVISGASRGIGLAIALGAARRGADVVLLAKTAEP
                     HPRLPGTVHTAVAEIEAAGGKGVAVVGDVRNEEDVARTVDTAVERFGGVDICVNNASA
                     IATEPTERLAAKKFDLMMDINVRGTFLLTKAALPHLRTSGASTRGAQAAGAHVLTLAP
                     PMNMNPHWLGAHPSYTLSKYGMTLLSLGWAAEYADAGIGFACLWPETYIATAAVINLA
                     DGDALAARSREPQIMGDAAVEIFSRPAAEVNGKCFIDSHVLAGAGQTDLSRYGGGDDP
                     ILDIFVDKS"
     gene            1347..>1620
                     /locus_tag="VGP27_21420"
     CDS             1347..>1620
                     /locus_tag="VGP27_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7853890.1"
                     /translation="MSISLLLEMATSSDPDRTAVVCGDTRLTTDELSGLADGGSGVIA
                     TCGAQHVAYVGTGGAMLPALLFSSARAAVPVTPLNYRLSADGLRELI"
BASE COUNT          299 a          522 c          546 g          253 t
ORIGIN      
        1 cctgccgacc tcctcgctga cctccggatc gtcggagtcg aagccgcact gcgtcggcag
       61 gtccagcgcg accgaaaggc ccgtcccgcc ctggtcgagc agataccggt aacggcggtt
      121 ggagtcctcc gcggtgccga atcccgagta ctggcggaac gtccacagct tgccccggta
      181 tccgcccgag aagttgccgc gggtgaacgg ataagcaccc ggcgcgggcg gttcaccggc
      241 ccgatcgccc ggcccgtaca cgggctgcag cgggatgccg gagggagtct gaactgggtc
      301 ggtcatcaat tgataacgta cttgcaaaaa atgagaatgc caataccgca cggcggaaac
      361 gcgcacggga cagggcgtgt tgaagcagct ggaagcagag cgaagacccg gtacacagtg
      421 attatggcac ttgcctaaaa tgagaacgtc aataccgatt tgttagggtg aggtttctcg
      481 acaggaggct tgatgtctcg tcagtcgctc accggtcgca ccatggtcat ctcaggggcc
      541 agccggggta tcgggctggc catcgcactc ggcgcggcac gacgcggcgc ggacgtcgtg
      601 ctgctggcca agaccgccga accgcacccg aggcttcccg gcaccgtgca caccgcggtc
      661 gccgaaatcg aggcagcggg cggcaagggc gtggccgtgg tcggcgatgt gcgcaacgaa
      721 gaggacgtgg cgcgcaccgt cgacaccgcc gtggagcgtt tcggcggcgt cgacatctgc
      781 gtcaacaacg ccagtgccat cgccaccgaa ccgacggagc ggctggccgc caagaaattc
      841 gatctgatga tggacatcaa cgtccgcggc accttcctgc tgacaaaggc cgcgctgccg
      901 catctgcgga cgtcgggggc gagcacgcga ggagcacaag cagcaggagc gcatgtgctg
      961 acgctggcgc cgccgatgaa catgaacccg cactggctgg gcgcgcaccc gtcgtacacg
     1021 ctctcgaaat acggcatgac gctgctgtcg ctgggctggg cggctgaata cgccgacgcc
     1081 ggcatcggat tcgcgtgcct ctggccggag acctatatcg cgaccgccgc ggtgattaat
     1141 ctggccgacg gggacgcgtt ggccgcccgc tcgcgcgaac cgcagatcat gggtgacgcc
     1201 gcggtcgaga tcttctccag accggccgcc gaggtgaatg ggaagtgctt catcgattcg
     1261 catgtgctgg ccggggcagg ccaaaccgac ctgtcccgct atgggggcgg cgacgacccc
     1321 atccttgata tcttcgtcga caaatcatga gcatctcctt gctgctcgag atggctacct
     1381 cgagcgatcc ggaccggacg gcagtagtct gcggcgacac gcggttgacc acagacgaac
     1441 tgagcgggct cgccgacggc gggtccggag tcatcgcgac gtgcggtgcg cagcacgtcg
     1501 cctacgtcgg caccggcggg gccatgctgc cggcgctgct cttctcgtcg gcacgcgccg
     1561 ccgtaccggt cacgccgctc aactaccggt tgagcgccga cggactgcgg gaactgatcg
//
LOCUS       DASYLX010000918         8640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1371144,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000918 DASYLX010000000
VERSION     DASYLX010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371144"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGP27_21425"
     CDS             complement(<1..268)
                     /locus_tag="VGP27_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7853891.1"
                     /translation="MSSTAASFAAGVRPDERLEVLFDELAELTGQRNAIDARIVEIFA
                     EVDRDELCGATGARSVAALVAWKTGSSSTNAHTIATVANRLGEFP"
     gene            complement(328..693)
                     /locus_tag="VGP27_21430"
     CDS             complement(328..693)
                     /locus_tag="VGP27_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7853892.1"
                     /translation="MLSVSRHIDAPAEAVWKLLVDTDAWPQWGPTVAGGEIDGGEMTL
                     GASGRVHTPVGVSLPFRITEFIPGRRWAWSVAGVPATSHSVEPDGSGCRASMAAPWWA
                     PAYLPVLAIALQRIDRMVR"
     gene            complement(711..2066)
                     /locus_tag="VGP27_21435"
     CDS             complement(711..2066)
                     /locus_tag="VGP27_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853893.1"
                     /translation="MSTVMDDRRRQAEHGSPGKVKTALGAAAGTCVENYDFVAYGTAS
                     ALYFGDAFFPDSDPTTGTLLSFATLGVGFLMRPIGGSVGGYLADRFGRKPVLVGALLV
                     MGIATVAIGCLPTYAQVGILAPVLLVVIRVIQGLAFGAEWGGAVLMAFEHAPWRRRGR
                     FAAIPQAGNPLGIALANIAFLAAAPLDSDWAWRIPFLASAVLIAVGLFVRLRLSESPE
                     FEAVKAEGKVVRNPFLTVIREDWRNILRIVALRVVESCAYYLTATYLLSYIAKRDPAD
                     RTIALTGVVIASIIAVGTTLYAGRLTDRVGRRRVYLAGCLLAIAFGIPMYLMANTGQP
                     FLIVLLFIIGIGVIHAVLTGTQGAWFAELFNTSTRTSGASIGYQVAASIAGFAPFLAV
                     LLANSFGWIGPALFYVAVGLVGLGGVVATKETWGPKQRAEVDALIRGDANADSVEAAP
                     A"
     gene            complement(2063..2551)
                     /locus_tag="VGP27_21440"
     CDS             complement(2063..2551)
                     /locus_tag="VGP27_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV7853894.1"
                     /translation="MRIALGNDHAGYPLKAYVRAVLERLGHEVIDTGTQDDAPVDFPD
                     ITWDTCKLVLLGEADKAVLVCGTGQGAVMAANKIPGIRCALAHDVYSAHQSVEHDDAN
                     AVAMGAWVIGQATAGEVIEAFLNASFDDDDDTVRRVRKLDELERNAARQLADELSKEI
                     TQ"
     gene            complement(2548..3465)
                     /locus_tag="VGP27_21445"
     CDS             complement(2548..3465)
                     /locus_tag="VGP27_21445"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV7853895.1"
                     /translation="MTDNRRPTRVFVVGSLNADHRVHVARFPQPGETVLASDIVISAG
                     GKGANQAVAAARAGATTTIIGAIGDDPDGEILARALSEQDIGTRDVRVVHGAQTGRAL
                     ITVDAGGENNIVVSPGANGELTTEDVDAGLSDIGERDLLLLQLETPEPLVRHAARVAS
                     DAGGVVVLNPTPVPDATQDLFEHVDLLVVNEHELAQLANDIATLASEADATVICTAGS
                     RGVFVTRQDRVEHIAASTVDAVDTTAAGDTFIGYLAARLAVAPDDILGAVRVAVSAAG
                     LAVTRSGAIASIPYDYELESDKTTMEGSR"
     gene            complement(3458..4465)
                     /locus_tag="VGP27_21450"
     CDS             complement(3458..4465)
                     /locus_tag="VGP27_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7853896.1"
                     /translation="MAGRGATIRDVAAHAGVSIGTASRVLSGHPATSSDARARVTQAA
                     TALGYRPNAQARSLRLTRTHAIGLLVSDVRNPFFADVAHAAEQAALGADYVTLLGNAN
                     ENVEQQDRYLETFLTQRVDGVVLAPQGRGSGSLEALIESRMPVVFVDRTVDGFDVPSV
                     TTDSRVGLDEAVAHLVAQGHTRIAYIGGPQSISTGRERYDALLEAFPRSGLDVDPVLI
                     KFGDFQEESGTRAAEQLLSGPNHPTAIVAADNLMALGALAAIRERGLRLGADIDVVAF
                     DDIEWLAHFDPPISVIAQDAGVVGRCAVELLLSVISGDDPESIVLPTRFINRSAGRDR
                     D"
     gene            complement(4555..5253)
                     /locus_tag="VGP27_21455"
     CDS             complement(4555..5253)
                     /locus_tag="VGP27_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853897.1"
                     /translation="MKTAVVTGGASGIGRAIAHRLEADGHHVATIDLQRSDGDFDYTA
                     DVTDREQVDAALNKIRTQLGPVSVLVNAAGLDGFSRFLDISFDDWKRVIDVNLNGVFH
                     CCQAVLPDMVDAGWGRIVNISSSSTHSGVARMTHYVSAKSAVNGLTKSLALEYGPSGI
                     TVNAIPPGFIDTPMLRSAEERGFTNVEKTIAMTPVRRMGRPEDIAAACAFLVSEEAGY
                     ITGQILGVNGGRNT"
     gene            complement(5250..5834)
                     /locus_tag="VGP27_21460"
     CDS             complement(5250..5834)
                     /locus_tag="VGP27_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853898.1"
                     /translation="MQTVAVQAPGAADTRQRLIDVAVDLFTRHSFAGTSLQMIADEMG
                     FTKAAIYYHFRTREELLAAVVEPIFQQLSGVITAAEAQRSATARADRMLRGYAELAVA
                     NRALVSVLACDPSVTTLLRRQPHWAEMINRQLALLAGDEDDPGGLVKATVVLAGISSA
                     VGPAWIAVDDGDLLGHLVETGRRTLGLRRKGIRS"
     gene            5985..7985
                     /gene="malQ"
                     /locus_tag="VGP27_21465"
     CDS             5985..7985
                     /gene="malQ"
                     /locus_tag="VGP27_21465"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV7853899.1"
                     /translation="MANPQPIPDDLRRLAAAHGVATSYRNERREPVQVDADVVIRVLG
                     LLDVEAGTEADRNAELAKLAERDRAGVIAPTIAVRLDGRRRPLPGAASLVGEDGSEMA
                     VRDELPADLSPGWYRLHTHDGQEATVVAAPSQVPASPATWGWMLQLYALRSARSWGIG
                     DLGDLREFVNWTAGEHGAGAVLLNPLHAPGPTHPVQPSPYTPSSRRFANPLALRIEDI
                     DAYRRADPDTRAEVDALRVSATTGRIDHDLVWASKRDALELLWRAEGRPSPFDDPSAS
                     KGLRDWATYCALAERHGGRWSRWPEPLRDVAGPAVASARRELAPRLAFHAWVQQRCAE
                     QLTAVRAAAHEAGMSLGVLHDLAVGVDADGADAWALADVLASGVSVGAPPDNFTPRGQ
                     DWGLPPWRPDRLAATGYAALRDMLRAVLAHADGLRIDHVAGLWRLWWIPPGDSPDRGT
                     YVHYDADTMLAVLALEAHRAAATVVGEDLGTVEPEVTEALAGNEMLGCAVAWFTRDES
                     APGEPLLPPAEWPSRAAASLSTHDLPTAAGFLRGEHVQVRADLGLLDDVVAEQAKADK
                     ERVEWLTLLRSEGLLPDVDHGAPEPDETAIIAAMHRFLASTPSRLKLISPYDVIAETR
                     QPNLPGTVDEYPNWRLPLPVTLEQLKADPRVADITAAFRAAT"
     gene            complement(8002..>8640)
                     /locus_tag="VGP27_21470"
     CDS             complement(8002..>8640)
                     /locus_tag="VGP27_21470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7853900.1"
                     /translation="LDELFNRVLHTEDDALRAARESAAAADMPAIEVSAQHGRLLSLL
                     ARMANAKRVLEIGTLAGYSTISLARGVGPDGRVVTLEFEPRHAAVARKNFERAGVAER
                     VEVIVGTALDTLPRLAERGETFDMFFIDADKENNVAYVEWAIKLGRPGSVVVVDNIAR
                     MGRVLDPAPDDHQARAVRDMFDMIGENPRLDAAAIQTVGTKNWDGFAVALIK"
BASE COUNT         1406 a         2884 c         2914 g         1436 t
ORIGIN      
        1 ggggaaactc accgagtcgg ttggccacgg tggcgatggt gtgggcattc gtcgacgacg
       61 agccggtttt ccaggccacc aacgccgcga ccgaacgcgc gcccgtggcg ccacacagtt
      121 cgtcgcgatc cacctcggcg aagatctcca cgatgcgcgc gtcgatcgcg ttgcgctgac
      181 cggtcaactc agccaactca tcgaacaaca cctcaagccg ctcgtcgggg cgtaccccag
      241 cggcgaaaga tgctgcagtg gaagacatga cgccatcatc gcacccaggt acgacagatc
      301 cgggagcgtc gcacgccgtg cacccggtca gcgcaccatc cggtcgattc gctgcagggc
      361 gatcgccaac accggcagat atgcgggcgc ccaccaggga gccgccatgc ttgcgcggca
      421 accgctgccg tcgggttcca ccgagtgcga ggtggcgggc acgcccgcga ccgaccacgc
      481 ccaccggcgt ccggggatga actcggtgat cctgaacggc agcgagacac cgaccggcgt
      541 gtgtacgcgt ccagacgcgc cgagcgtcat ctcgccgccg tcgatctccc cgccggcgac
      601 ggtcggtccc cattgcggcc acgcgtcggt gtccacgaga agcttccaca cggcctcggc
      661 cggtgcatcg atgtgtctac tgacggagag cattggggcg gcatacccag ttacgcgggc
      721 gcggcctcca cggagtcggc gttcgcgtcg ccgcgaatca gcgcgtcgac ctcggcacgc
      781 tgcttcgggc cccaggtctc cttggtcgcg acgacaccac cgagtccgac gagcccgacc
      841 gcgacgtaga acaacgcggg tcctatccag ccgaagctgt tggcgaggag caccgccagg
      901 aacggcgcga atccggcgat tgacgcggcg acctgatagc cgatggatgc acccgaggtt
      961 cgcgtactgg tgttgaacag ttcggcgaac caagcgccct gcgttccggt cagcacggcg
     1021 tggatgacgc cgatgccgat gatgaacagc agcacgatca ggaacggttg gccggtattg
     1081 gccatgagat acatcggaat gccgaacgcg atggcaagca gacaacccgc cagatacacc
     1141 cgtcggcgtc cgacccgatc ggtcaggcgt cccgcgtaca gcgtcgtgcc gaccgcgatg
     1201 atgctggcga tgacgactcc ggtcagtgcg atcgtgcgat ccgccggatc gcgcttggcg
     1261 atgtacgaca acaggtatgt ggccgtgagg tagtaggcgc acgactcgac gacccgcagc
     1321 gcgacgatcc gcaggatgtt gcgccagtcc tcgcggatca cggtgaggaa cggattccgg
     1381 acaaccttgc cctccgcctt gacggcctcg aactccggcg actcactcag cctcaaacgc
     1441 acgaacaggc cgacggcgat gaggacggcg ctagccaaaa acgggatgcg ccaggcccaa
     1501 tcgctgtcga gcggtgccgc ggccaggaag gcgatgttgg cgagcgcaat accgagtgga
     1561 ttgccggcct gcgggatcgc cgcgaatcgg ccccttcgcc tccagggtgc gtgttcgaac
     1621 gccatcagta cggcgccgcc ccactccgcg ccgaacgcga gaccctggat cacccgaatg
     1681 accacgagta gcaccggggc cagaattccg acctgcgcgt aggtgggcag gcacccgatc
     1741 gccaccgtcg cgattcccat caccagcagt gctcccacga gcaccggctt gcggccgaat
     1801 cggtctgcga gatagccgcc gacactgcca ccgatcggac gcatgagaaa gcccacgccc
     1861 agggtggcga atgacaggag ggtgccggtt gtcgggtcgg agtctgggaa gaacgcatcg
     1921 ccgaagtaga gagcggaagc ggttccgtac gccacgaagt cgtagttctc gacgcaggtt
     1981 cccgccgccg cacccaatgc agtcttgacc ttgcccggcg agccgtgttc agcctggcgc
     2041 cggcgatcat ccatgacggt gctcactgtg ttatctcctt tgataattcg tcggccagct
     2101 gccgagccgc attgcgctcg agctcatcca gcttgcggac gcggcggacg gtgtcgtcgt
     2161 cgtcgtcgaa ggatgcgttg aggaaggcct cgatgacctc gcccgcggtt gcctgcccaa
     2221 tcacccacgc gcccatcgcg actgcattcg catcgtcgtg ttcgacactc tggtgtgcgg
     2281 aatacacgtc atgggcgagc gcacagcgaa tccccggaat cttgttcgcc gccatgacgg
     2341 cgccttgacc cgtcccgcag acgagcacgg ctttatcggc ctcaccgaga agtacgagct
     2401 tgcaggtgtc ccaggtgatg tcgggaaagt cgaccggcgc gtcgtcttgc gtgccggtgt
     2461 cgatgacctc gtgaccgagc cgttccagca ctgcgcgtac gtacgccttg agcgggtatc
     2521 ccgcgtgatc gtttccgagt gcgattctca tcgcgatcct tccattgtcg ttttgtcgct
     2581 ttcgagttcg tagtcgtacg ggatggaggc gatggcgccg gagcgtgtga cagcgaggcc
     2641 ggccgctgag acggcgactc tgaccgcgcc gaggatgtcg tcgggtgcga ccgcgaggcg
     2701 cgccgcgagg tagccgatga aggtgtcgcc tgccgcggtg gtgtcgacgg catcgaccgt
     2761 tgatgccgcg atgtgctcaa cgcggtcctg gcgtgtgacg aatacgccgc ggctgcctgc
     2821 ggtgcagatc accgtggcgt cggcttcgct tgccagcgtt gcgatgtcat tcgcgagctg
     2881 agcgagttcg tgttcgttga cgacgagcag gtccacatgt tcgaacaggt cctgcgtcgc
     2941 gtcgggcact ggggtcgggt tgaggaccac cactcccccg gcatctgatg cgacgcgcgc
     3001 ggcgtgcctc accagcggct cgggagtctc aagttgtagg agcagcaggt cgcgctcgcc
     3061 gatgtcgctc aggccggcgt cgacgtcctc ggtggtgagt tctccgttgg cgccgggtga
     3121 gaccacgata ttgttctcgc cgccggcatc caccgtgatg agcgcccgcc ccgtctgcgc
     3181 tccgtggacg acacgcacat ctcgggtgcc gatgtcctgt tcggacagtg cgcgcgccag
     3241 gatttctccg tcggggtcgt cacctattgc gccgatgatc gtcgtggtcg cgccggcccg
     3301 tgccgcagcg accgcctggt tagcgccttt gcctcccgcg gaaatgacga tgtcagacgc
     3361 cagcaccgtc tcgcccggct gcggaaacct ggcaacgtgg actcggtggt cggcgttgag
     3421 gctgccgact acgaacaccc gcgtgggtcg gcgattgtca gtcacggtcg cggccggccg
     3481 atctattgat gaatcgtgtt ggcagcacga ttgattcggg gtcatcaccg cttatgacgc
     3541 tcaacagcag ttcgacggcg catcgcccca cgacgccggc atcctgcgcg atcaccgaaa
     3601 tcggcggatc gaagtgcgcg agccactcga tatcgtcgaa ggccaccaca tcgatgtccg
     3661 cgcccagccg caggccacgc tcccggatgg ccgcgagtgc gcctagcgcc atcaggttgt
     3721 cggcagccac gatggcggtc gggtgattcg ggccggacag caattgctcg gccgcccgag
     3781 taccgctttc ctcctggaag tcgccgaatt tgatcagcac cgggtcgacg tccagaccgc
     3841 tccgcgggaa cgcctccagt aaggcgtcat agcgttcacg cccggtggag atcgactgtg
     3901 gaccaccgat ataggcgatg cgggtgtggc cctgcgcgac gaggtgtgcg acggcctcgt
     3961 caaggccgac acggctatcg gtggtcacac tcggcacatc gaatccgtcc accgtgcggt
     4021 cgacgaacac cacgggcatc cgactctcga tcagcgcctc gagacttccc gaaccgcgcc
     4081 cctgcggtgc cagcaccacg ccgtcaacac gttgggtgag gaaggtttcg aggtaacggt
     4141 cctgctgctc gacgttctcg ttggcgttcc cgaggagggt cacgtagtcg gctcccagag
     4201 cggcctgctc cgcagcgtgc gcgacgtcgg cgaagaaggg gttgcgcaca tcggagacca
     4261 gaagaccgat ggcatgcgtg cgggtgagcc ggagggaacg cgcctgcgcg ttggggcggt
     4321 agccgagcgc ggttgctgcc tgggttaccc gggcgcgcgc atcggaagag gtcgcggggt
     4381 ggccggataa cacgcgcgag gcggtgccga tcgaaacgcc ggcgtgtgct gcgacgtcac
     4441 gtatggtggc gcccctacct gccactatct gcacctcaat ccgtgggaac gtttccatgg
     4501 gaacgtttcc acgtaactta ggtcacatct ggcggccccg tcaaccgccc ctcgtcaggt
     4561 gttgcgcccg ccgttcacac cgagaatctg cccggtgatg tagccggcct cctcggagac
     4621 caggaaagcg cacgccgcgg cgatgtcttc gggcctgccc attcggcgca ccggcgtcat
     4681 cgcgatcgtc ttttccacgt tggtgaagcc gcgctcctcg gcgctgcgca gcatgggggt
     4741 gtcgatgaag ccgggtggta ttgcgttgac ggtgatgccg ctgggcccgt actccagcgc
     4801 cagcgatttg gtcagcccgt tcacggccga tttggcggac acgtagtgcg tcatgcgcgc
     4861 cactcccgag tgtgtgctcg acgaactgat gttcacgatg cggccccagc cggcgtcgac
     4921 catgtcgggc agcacggcct gacagcagtg gaagacgccg ttgagattga cgtcgatcac
     4981 ccgcttccag tcatcgaacg agatgtcgag gaagcgtgag aagccgtcca ggccagcggc
     5041 attgacgagg accgacaccg gaccgagctg tgtgcggatc ttgttgagtg cggcgtcgac
     5101 ctgttcacga tcggtgacgt cggcggtgta gtcgaagtca ccgtctgagc gctggaggtc
     5161 gatcgtggcg acgtgatgac cgtcggcttc cagtcggtgc gcgatggcgc ggccgatgcc
     5221 cgacgccccg ccggttacca cagctgtctt catgaccgga ttcctttcct gcgcaacccg
     5281 agggtgcgcc gtcctgtttc cacgagatgc cccagcaggt cgccgtcgtc gacggcgatc
     5341 catgccggac cgacggcgct cgagattcct gcgagcacca cggtggcctt gaccagcccg
     5401 ccgggatcgt cctcatctcc ggcgagcagc gccagttgcc ggttgatcat ctccgcccag
     5461 tgtggctgcc tgcgaagcag ggtcgtcacg ctggggtcgc acgcgagcac cgagacgaga
     5521 gcacggttgg ccacagccaa ttcggcgtag ccgcgcagca tgcggtcggc gcgtgcggtg
     5581 gcgctgcgct gcgcttccgc ggccgtgatg acgccgctga gctgctggaa gatcggttcg
     5641 acgacggcgg cgagcagttc ctcgcgggtg cggaagtggt agtagatggc ggcctttgtg
     5701 aagcccatct cgtcggcgat catctgcagc gaggtacccg cgaagctatg ccgggtgaac
     5761 aggtcgacgg cgacgtcgat caggcgctgc ctggtgtcgg ctgcaccggg cgcctgaacc
     5821 gcgacggtct gcatacttta cgaccgtaca ggagagtact tgccgatcgt aaagcgggcg
     5881 gcacagcacc ggcttgagaa atcacctcct gggtatagca gccgcgtgct tccacttcgc
     5941 cgggtcgtcg acgtccgttc cgcagcactt atgagggtcg ggtgatggcg aacccgcagc
     6001 cgattcccga tgacctgcgc cggttggccg ccgctcacgg ggtcgcgacc agctaccgga
     6061 acgagcgccg cgagccggtg caggtcgacg ccgatgtggt gatccgggtg ctcggactgc
     6121 tcgatgtcga ggccgggacc gaggcagacc gcaacgcaga gctggccaag ttggcggagc
     6181 gcgaccgcgc cggggtgatc gcgccgacca tcgccgttcg gctggatggg cgccgacgac
     6241 cgctgcccgg ggccgcttcg ctggtgggcg aggacggcag cgagatggcg gtgcgcgatg
     6301 agctgccggc tgacctttcg ccgggctggt accggctgca cacccacgac gggcaggagg
     6361 cgaccgtagt cgcggcgccg tcccaggttc cggcttcacc cgccacgtgg ggctggatgc
     6421 tgcagctcta cgcgctgcgc tcggcccgtt cgtggggcat cggcgatctc ggcgatctac
     6481 gcgaattcgt gaactggacc gcgggcgagc acggcgccgg agcggtgctg ctgaacccgc
     6541 tgcacgcgcc gggtcccacg catccggtgc agccctcgcc ctacacgccg tccagccggc
     6601 gcttcgccaa cccgctggcc ctgcgcatcg aggacatcga cgcctaccgc cgagccgacc
     6661 cggacacccg tgcggaggtg gacgctctgc gggtgtccgc gaccacgggt cgtatcgatc
     6721 acgacctggt atgggcgtcc aaacgcgatg cgctggaact gctttggcgg gccgagggcc
     6781 gacccagtcc cttcgacgac ccgtcggcgt cgaaggggct gcgggactgg gcgacatact
     6841 gcgcgctggc cgagcggcac ggcggccgat ggtcacgctg gcccgaaccg ctgcgcgacg
     6901 tcgcaggccc ggccgtggca tccgcgcgcc gggagttagc gccgcggttg gcgtttcacg
     6961 cctgggtgca gcagcgatgc gccgaacaac tcaccgccgt tcgggctgcc gcgcacgagg
     7021 cagggatgtc gctcggcgtg cttcacgacc tcgcggtggg cgtcgacgcc gacggtgccg
     7081 acgcgtgggc gctggccgac gtgctcgcgt ccggcgtcag cgtcggcgcg ccgccggaca
     7141 acttcacccc gcggggccag gactgggggc tgccgccgtg gcgtccggat cgcctggcag
     7201 ccaccgggta cgccgccctg cgggacatgt tgcgcgccgt actcgcccac gccgacgggt
     7261 tgcggatcga ccatgtcgcg ggcctgtggc gactgtggtg gatcccgccg ggcgacagtc
     7321 ctgatcgagg cacgtacgtg cactacgacg ccgacaccat gctcgcggtg ctggcgctgg
     7381 aagcgcaccg ggccgcagca accgtcgtcg gcgaggacct cggcaccgtc gaaccagaag
     7441 tcaccgaggc gctggccggt aacgagatgc tggggtgcgc ggtggcctgg ttcacccgtg
     7501 acgagtccgc acccggcgaa ccgcttctgc cgccggccga atggccgtcg cgagcggccg
     7561 ccagcctgtc cacgcatgac ctgccgaccg cggcgggttt cctgcgcggt gaacatgtcc
     7621 aagtgcgtgc cgacctcggg ctgctagacg acgtcgtggc cgagcaggcc aaagccgaca
     7681 aggaacgcgt ggagtggctg accctgttgc gttccgaggg gctgcttccc gacgtggacc
     7741 acggcgcgcc ggaaccggac gaaacggcga tcatcgccgc gatgcaccgg ttcctcgcgt
     7801 cgacgccgag caggctcaag ctgatctcgc cctacgacgt gatcgccgag acccggcagc
     7861 cgaatctgcc tgggacggtt gatgaatatc cgaactggcg actcccgttg ccagtgacgt
     7921 tggagcaatt gaaggccgac cccagggttg ccgacatcac tgcggcgttc cgggcggcga
     7981 cctgatcagc acgcgcggaa gctacttgat cagcgccacc gcgaagccgt cccaattttt
     8041 ggtgccgacc gtctgaatcg ccgccgcgtc caggcgcgga ttctccccga tcatgtcgaa
     8101 catgtcgcga acggctcgcg cctggtggtc gtcgggcgcc ggatccagca cccggcccat
     8161 gcgagcgatg ttgtccacca cgacgactga acccggcctc cccagcttga tggcccactc
     8221 gacataggcg acgttgttct ccttgtcggc gtcgatgaag aacatgtcga acgtctcgcc
     8281 gcgttccgcc aatcggggca atgtgtccag cgcggtcccg acgatcacct cgacgcgctc
     8341 ggcgacaccg gctcgctcga agttcttccg cgccaccgcc gcgtgccgcg gctcgaattc
     8401 gagtgtgacg acccgcccgt cggggcccac gccgcgcgcg aggctgatgg tgctgtaacc
     8461 ggccagcgtg ccgatctcca ggacccgctt cgcgttcgcc atcctcgcca acagcgacag
     8521 cagcctgccg tgctgggccg acacctcgat ggcgggcatg tccgccgccg cagccgattc
     8581 ccgcgcggcg cgcagcgcgt cgtcctcggt gtgcaacacc cgattgaaca gctcatcaag
//
LOCUS       DASYLX010000919         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1811034,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000919 DASYLX010000000
VERSION     DASYLX010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811034"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VGP27_21475"
     CDS             <1..692
                     /locus_tag="VGP27_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7853901.1"
                     /translation="WRRGNVFLLGDAAHLTPPFIGQGMGAGLRDAMNLSWKLAGVLAG
                     ELPSAVLDTYQQERKPHTLALIRLALNVGRSMTCGGDFGNLIRRIVVPRIHHIPGLRD
                     KVVDSTTPALRRSALVHRSRRRRRLTGTLCPNPLLPDGHRLDDAIGTGFALISAVPVE
                     AEHRARLKQHGAAVQIAETGTHLAEWLRRGRSTAAIVRPDRTVMRTGRRVDELCAAVP
                     VFTADGGRRQC"
     gene            686..1876
                     /locus_tag="VGP27_21480"
     CDS             686..1876
                     /locus_tag="VGP27_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853902.1"
                     /translation="MLTKAPSGVPAYRANIYTTDAIVDPYPHYARLREQGAVVWMSRH
                     RLYALPRYAECKSVLRDDKTFISGKGVALNPFSNRLSRGTTLTSDGAEHDQRRKLVAH
                     RMLPRALRAISDSVDRLAADVVAAALDRGEVDGVHDLAYALPLAVVPDLVGWPRDRRD
                     NLLRWGAATFDILGPLNRQAIRSALPSLQMLRFARRVVRTRSLVEGSMAHETLTAADE
                     GKISHAECAPLMIDYIAPSLDTTISSISSALYLFATHPEQWRILKDDPSLIPNAVNEV
                     IRFESPLRAFARHTRCDTDIADVRIPAGARVLVIYASANRDEREWHEPDMFDIRRDAN
                     RQLGFGQGAHACAGQGLARLETSAILQALVDRVDGIELTAEPTWAVNNIIRRHERMPL
                     KLIP"
     gene            1940..2431
                     /locus_tag="VGP27_21485"
     CDS             1940..2431
                     /locus_tag="VGP27_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7853903.1"
                     /translation="MDDAATLLEIEAIKQLKARYCRLLDTKDWQAWRSLFSDDFLSDT
                     SKAGGKVIRGADEFVAFTRKGVRSQATVHQVHAPEIELTSPTTARGVWALEDIVRFGP
                     AVNLRGYGHYTETYEKVDGQWTFTSSALTRLREDVGNGVVSVFVSDRFRRAIGKLSRR
                     VMK"
     gene            2428..3246
                     /locus_tag="VGP27_21490"
     CDS             2428..3246
                     /locus_tag="VGP27_21490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7853904.1"
                     /translation="MTDAPVLYPLNTALGRFGIANVVDSPDPFVSSMPAAGLVNPLTG
                     VPTAGPLAVLVDHVAGLVNHYRRQADEWTVSTELSLELTPDALTVIADRPEVPVVATG
                     RPVGATGPSAFGLCELTIDDTVIGVGTVRSVYIGHPGEFPQEWPTMPVDAPRPTELAD
                     LMAVRIGDGNGSPVLHQERDPVLNNNLAIVHGGVAAAGLELAASAALNADRPDQPLNT
                     ASLRVNFLRQFFSGDRSRYAGTALRIGRRSGVAEAQAVGTDGEVALVGRVTAYR"
     gene            complement(3250..4170)
                     /locus_tag="VGP27_21495"
     CDS             complement(3250..4170)
                     /locus_tag="VGP27_21495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7853905.1"
                     /translation="MRFTFTHPMHSHPYNPELVTGAGIATVAAAAEAAGFHGFGFTDH
                     PAPSQRWLESGGHDAVDPFVAMGFAAARTTTLRLIPNIVVLPYRNPFVVAKSGATLDF
                     LSNGRFTLAVGVGYLKREFAALGVDFDERAALFEEALEVIRGIWTTDDFSFEGRHFSA
                     KGITAHPRPVSKPHPPIWIGGNTSAARQRVAAFGDGWCPFPAPAVLAQTARTAALDSV
                     DALVDGISDLRRRCDAAGRDWSAIDITFSNIGGGSPGDQDFIADAYMAGLEKLAAVGV
                     TWVQVQVPGDSLQHAVDAIGRFGSSVIASA"
BASE COUNT          763 a         1453 c         1355 g          665 t
ORIGIN      
        1 aatggcggcg cggcaacgtc tttctgctcg gcgacgccgc ccacctcacc ccgccgttca
       61 tcggacaggg catgggcgcc gggctgcgcg atgcgatgaa cctgtcctgg aagctcgcag
      121 gcgtgctggc cggcgagctc ccatcggccg tcctcgacac ctaccagcag gagcggaagc
      181 cacacaccct tgcgttgatc cggctcgcgc tcaatgtcgg ccggtcgatg acgtgtggcg
      241 gcgacttcgg caatctgatc cgccgcatcg tcgtaccgcg gatacaccac atccccggcc
      301 tgcgcgacaa ggttgtggac agcacgacgc ctgcactgcg ccggtccgcg ttggtccacc
      361 gatcacgcag gcggcggcgc ctgacgggca cactctgccc caatcccctg ctgcccgacg
      421 gccaccggct cgacgacgcc atcggcaccg gcttcgctct catctcggcc gtcccggtgg
      481 aggccgaaca ccgagcacgc ctgaagcaac acggggccgc agtccagatc gcagagaccg
      541 gaacccatct cgccgagtgg ctgcggcgtg gccgctcgac ggcggcgatc gtgcgacccg
      601 accgcaccgt gatgaggacg ggccgacgcg tcgacgagct gtgcgccgca gtaccggttt
      661 tcacggcgga cggaggacga cggcaatgct gaccaaggcg cccagtgggg ttcccgcgta
      721 ccgggcgaac atctacacca ccgacgcgat cgtcgacccg tacccccact atgcgcgact
      781 acgtgagcag ggcgctgtgg tgtggatgtc gcgccaccgg ctctacgcgt tgccacggta
      841 cgccgaatgc aagtccgtgt tgcgcgacga caagacgttc atctcgggca agggggtggc
      901 actgaacccc ttcagcaacc ggctgtctcg cggcaccacg ctcaccagtg acggcgcgga
      961 acacgatcag cgccgcaagc tggtcgccca ccggatgctc ccacgggcct tgcgggccat
     1021 cagcgacagc gtcgaccggt tggctgccga cgtcgtcgcg gcggcactcg atcgaggtga
     1081 ggtcgatggc gttcacgatc ttgcgtacgc gttgccgctc gcggtggtcc ccgacctcgt
     1141 cggctggccg cgggatcgcc gggataatct gttgcgttgg ggggcagcga cattcgacat
     1201 acttggcccg ctgaacaggc aagcgatcag gtcggccctt cccagccttc agatgctgcg
     1261 attcgcccgc cgagtggtgc gtacccgcag ccttgtcgag ggcagcatgg cacacgaaac
     1321 tctgaccgcc gctgacgaag gcaaaatctc gcacgctgaa tgcgcgccgc tgatgatcga
     1381 ttacatcgcg ccgtcactgg acaccacgat cagctcgata tcgagtgcgc tgtacctgtt
     1441 cgccacccat ccggagcagt ggcggattct caaggacgac ccgtcgttga tccccaacgc
     1501 ggtcaacgag gtgatccgct tcgaatctcc gctgcgcgcc ttcgcccgcc acacccgttg
     1561 cgacaccgac atcgcagatg tccggatccc cgcaggcgcg cgggtgctgg tgatctacgc
     1621 atcggccaac cgagacgaac gcgaatggca cgaaccggac atgttcgaca tccgccgcga
     1681 cgccaaccgc cagctcgggt tcggccaggg cgcacatgcg tgtgccggac agggcctggc
     1741 acggttggag acctcggcga tcctgcaagc tctggtcgat cgcgtcgacg ggatcgaact
     1801 tacggcggag ccgacctggg cggtcaacaa catcatccgg cgccacgagc gcatgccgct
     1861 caagctcatc ccctagtgcc ctcgcttcag agtcaatcag caactttaca atcgttgacc
     1921 agaatgtcac gctctcggga tggatgacgc cgccaccctg ctggagatcg aggcgatcaa
     1981 acagctcaag gcccgctatt gccgactgct ggacaccaag gactggcagg cctggcgaag
     2041 cctgttcagc gacgacttcc tcagtgacac gtcaaaggcg ggcggcaagg tgatccgtgg
     2101 cgccgacgaa ttcgtcgcct tcacccgcaa gggtgtgcga tcgcaggcca cggtgcatca
     2161 ggtgcacgca cccgagatcg agctgacgtc accgacgacc gcgcggggcg tctgggcgct
     2221 cgaggacatc gtccgattcg ggcctgccgt gaacctgcgc ggctacggcc actacaccga
     2281 gacatacgag aaggtcgacg ggcagtggac cttcaccagc tccgcgctga cccgactgcg
     2341 ggaagatgtg ggcaatggcg ttgtctcggt gttcgtttcg gaccgattcc gacgggccat
     2401 cgggaaactg tcccgccggg tgatgaagtg accgacgcac ccgtcctgta cccgctcaat
     2461 acggctctgg gccgattcgg catcgcgaac gtcgtcgaca gccccgatcc cttcgtcagt
     2521 tcgatgccgg ccgcgggcct ggtcaatccg ctgaccggcg tcccgaccgc cggaccgctg
     2581 gcggtcctgg tcgaccacgt ggcgggcctc gtcaaccact accggcggca ggccgacgag
     2641 tggaccgtat ccaccgagct gtcgctggag ctgacccccg acgccttgac cgtcatcgcc
     2701 gacaggcccg aggttccagt tgtcgcgaca ggccggcccg tcggggccac gggcccatcg
     2761 gcattcggtc tgtgcgagtt gacgatcgac gacaccgtta tcggggtcgg aactgtccgg
     2821 tctgtctaca tcgggcaccc aggtgaattc ccgcaggagt ggcctacgat gcccgtcgac
     2881 gcccctcgac cgacggaact cgccgacctc atggcggtgc ggatcggcga cgggaacgga
     2941 tcgccggtgc tccaccaaga acgggatccg gtgctcaaca acaacctcgc catcgtgcac
     3001 ggcggagtgg cggccgcggg tctggagctc gcggcctccg cggcactcaa cgccgaccgc
     3061 cccgaccagc cgttgaacac cgcgtcgcta cgggtgaact ttctgcgcca gttcttcagc
     3121 ggcgatcgat cacgttatgc gggaaccgcc ctgcgcatcg gcaggcgcag tggtgtggcc
     3181 gaggcgcagg cggtcggcac cgacggtgag gtcgccctcg tcggcagggt taccgcatac
     3241 cggtgagcgt cacgcggacg cgatgaccga gctgccgaac cgcccgatcg catccaccgc
     3301 atgctgcagg ctgtcaccgg gcacctggac ctgaacccat gtgacgccca ccgccgcaag
     3361 cttttccaaa ccagccatgt acgcgtccgc aatgaagtcc tgatcgcctg gcgatccgcc
     3421 accgatgttg ctgaaggtga tgtcgatggc cgaccagtcc cggcccgccg cgtcgcagcg
     3481 ccggcgcagg tcggagatac cgtcgacgag agcatcgacg gagtccagtg ccgcggtgcg
     3541 ggcagtctga gcaaggaccg caggcgcggg aaaagggcac cagccgtcgc cgaaagccgc
     3601 cacgcgttgc cgggccgcgg acgtgttgcc gccgatccag atcggcggat gaggcttgct
     3661 caccggacgc gggtgagcgg tgatgccctt ggcggagaag tgacggccct cgaacgagaa
     3721 gtcgtcagtg gtccagatgc cgcgaatcac ctcgagcgcc tcctcgaaca gcgccgcccg
     3781 ctcatcgaaa tcgacaccga gagcggcgaa ttcacgcttc aggtagccga cacccacggc
     3841 gagggtgaac cggccattgg aaagaaaatc cagcgtcgca ccggacttcg cgaccacgaa
     3901 cggattgcga tacggcaaca cgacgatgtt ggggatcagt cgcagcgtcg tcgtacgggc
     3961 cgcggcgaag cccatggcga cgaacgggtc caccgcatcg tgaccgccgg actccagcca
     4021 gcgctgtgac ggtgccggat ggtcggtgaa accgaacccg tgaaagccgg ccgcctccgc
     4081 cgccgccgcg accgtcgcga tacccgcccc ggtcaccaac tccggattgt atgggtggct
     4141 gtgcatcggg tgggtgaacg tgaaccgcat caacggccgc cttccagaat taccttctcc
     4201 aaagttgaga atgtcgttac catgccactc atatga
//
LOCUS       DASYLX010000920         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3457473,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000920 DASYLX010000000
VERSION     DASYLX010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457473"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VGP27_21500"
     CDS             <1..772
                     /locus_tag="VGP27_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7853906.1"
                     /translation="YLRSASLDDRRYLLFNPVTKAKVTMEGENIMRALHDVVAAKGYE
                     KNTMFREVAQLIGALPRLEGTVHVNVGLVLKFMPNYMLNPTEYPEIPTRNDPADDEFF
                     WNQGPTRGAGKVQFADWAPAYEKHLDIPNVARFYEQAQAFKQLLLTATPDAEQQKDLD
                     FLLNVGHLFSLIVYGQLILEQATLTGVDDDIVDQIFDFQIRDFSTYAVALHGKSSSTQ
                     SQQDWALGAIRKPVSDAQRFDRVWERVKAYNGAYEMRP"
     gene            complement(783..1892)
                     /locus_tag="VGP27_21505"
     CDS             complement(783..1892)
                     /locus_tag="VGP27_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7853907.1"
                     /translation="MPEKPSAVPESSVVVRPEPIDSETYTVSSRLQAAGLRPAIALFE
                     QAADTVPIPRPPQPIVIADYGASTAHNSLLPISAALPVLRNRTRPDHSILVVHTDVPD
                     NNFSVMFDTLSDDPDTYLRKDPATFASAVGRSFYSQIIPSNSVHLGWSSWAIQWLSRV
                     PAPIPDHVQVDYSADDNVRTAYAKQAAHDWHEFVAFRGRELCPGGQLVVLTMALDEHG
                     EFGYRPLLDALVEVLGELTADGLLTEQEVRRMCLPAVGRRKADFLAPFAPSGRFERLS
                     IEHLEVFNAEDRFWMQYQVDHDARAFAALWAAFVRSAIFPTLVTALADGRNDPRSSQL
                     FDRLEAGISERLSANPEQMQIPFAKLVLTKQGRTK"
     gene            2011..2745
                     /locus_tag="VGP27_21510"
     CDS             2011..2745
                     /locus_tag="VGP27_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV7853908.1"
                     /translation="MTGRRTAPRIFILASILTVVVAVVGFIVTMVLNAFVFDEYDAYG
                     EVPIPGSSSLHLPAGQATVSFHTQTIGSPSGGGLPVPQLGLTISPPEGVAEPEVTQSW
                     GTTTSVNNDAHIRVWAVRIPAEGTYHVTTDGNVNGYINPQLAFGHDSSNSTLMWAFAG
                     LFVIGLLQLTAAITYAVKSGKRPTPAVPVQSTFDVGDSYGIRDPHKSADDGIRLEQLR
                     TLAGLRDSGALTEAEFQAEKRRILNT"
     gene            complement(2836..3063)
                     /locus_tag="VGP27_21515"
     CDS             complement(2836..3063)
                     /locus_tag="VGP27_21515"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEV7853909.1"
                     /translation="MPFINVKVIEGVFSAAQKQDIVRSVTNAMVEIEGENMRPVTWVV
                     VEEIASGDWGVGGTPLHTADVKALAAGHPVG"
     gene            complement(3080..3700)
                     /locus_tag="VGP27_21520"
     CDS             complement(3080..3700)
                     /locus_tag="VGP27_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7853910.1"
                     /translation="MTRYKINSARVDPASSPLPADIQDSIDAIMRGNDPLVLFTTLAR
                     DRRLFYKLFSAGLLDRGHLDIRQREIVIDRVTAHCGAEYEWGVHVAAYATKAGLDDRQ
                     IASLVRDHASEDLWADEDRVLIALCDSLRDFCTVDDDLWDSLTNYYSDEAIIELLMLA
                     GFYRMVSYLVNGLRLPLEPNTPRFADLPDVGQRDGSGQQFRWPAGE"
BASE COUNT          697 a         1233 c         1249 g          679 t
ORIGIN      
        1 ctacctgcgc agcgcgagcc tcgacgacag gcgctacctg ctgttcaatc ccgtcaccaa
       61 agccaaggtc acgatggagg gcgagaacat catgcgggcg ctgcacgatg tggtcgccgc
      121 caagggatat gagaagaaca cgatgttccg cgaggtggcg cagctcatcg gcgcgctgcc
      181 ccgactggag ggcacggtgc acgtgaacgt gggcctcgtg ctgaagttca tgccgaacta
      241 catgctcaac cccacggagt atccggagat cccgacccgc aacgatcccg ccgacgacga
      301 gttcttctgg aatcagggac cgacacgcgg cgccggcaag gttcagttcg ccgactgggc
      361 cccggcgtac gagaagcatc tcgatatccc gaacgtcgcg cggttctacg agcaggcgca
      421 ggcgttcaag cagttactgc tcacggccac cccggatgcg gagcaacaga aggatctgga
      481 cttcctgctc aacgtcgggc atctcttctc gctgatcgtg tacggccagc tgatcctcga
      541 gcaggcgacg ctgaccggcg tcgacgacga catcgtcgac cagatcttcg acttccagat
      601 tcgcgacttc agcacttacg ccgttgcctt gcacggtaag tcgagctcaa cgcagtcgca
      661 gcaggattgg gcgctcggtg cgattcgcaa accggtctcc gatgcacagc gcttcgaccg
      721 ggtgtgggag agggtcaagg cctacaacgg cgcctacgag atgcgtccct gacgggatcg
      781 cgtcacttgg tcctgccttg tttggtcagc accagcttgg cgaacgggat ctgcatctgc
      841 tccgggttgg ccgacagccg ttcactgatg cccgcctcca gccggtcgaa caactgcgac
      901 gaccgtgggt cgttgcggcc gtccgcgagc gcggtgacca acgtggggaa tatcgcagac
      961 ctcacgaagg cggcccacag cgcggcgaag gcgcgtgcgt cgtggtcgac ttggtactgc
     1021 atccagaacc ggtcctcggc gttgaacacc tcgaggtgtt cgatggagag ccgctcgaac
     1081 cggcccgacg gcgcgaacgg ggccaggaaa tccgccttcc gtcggccgac ggcaggcagg
     1141 cacatgcgac gcacttcctg ctcggtcagc agaccgtcgg cggtcagttc gccgagcacc
     1201 tcgaccaggg cgtcgagcag cggccggtaa ccgaactcgc cgtgctcgtc gagggccatc
     1261 gtcagcacca ctagttggcc tcccgggcac agctcgcgac cgcggaacgc gacgaactcg
     1321 tgccagtcgt gcgcggcctg cttcgcgtat gccgtgcgca cgttgtcgtc ggcgctgtaa
     1381 tccacctgca catggtcggg gatgggtgcg gggacccgac tcagccactg aatcgcccac
     1441 gacgaccagc ccaggtggac gctgttcgac gggatgatct ggctgtagaa cgaccgcccg
     1501 accgcggacg cgaacgttgc ggggtccttg cgcagatacg tgtcggggtc gtcggagagc
     1561 gtgtcgaaca tcacggagaa gttgttgtcc ggtacgtcgg tgtgcacgac caggatcgag
     1621 tggtccggcc gagtccgatt gcgcagcacc gggagtgccg ccgagatggg cagcaacgag
     1681 ttgtgtgcgg tcgacgcgcc gtagtcggcg atgacgatcg gctgcggcgg ccgggggatc
     1741 ggcaccgtgt cggcggcctg ctcgaaaagc gctattgcgg gacgcagtcc agcggcctgc
     1801 aaccgcgagc tgacagtgta ggtctcgctg tcgatcggct cgggtcggac caccacgctc
     1861 gactcgggta cggccgacgg cttctcgggc atggcgacaa cggtagccgt cgtcgtcgca
     1921 cagcggccag ttcggttacg gggtccgggc cggggtggcg acctggatga ttggcgccgc
     1981 ggcgcgaagg gagcgtgaca gactgcgccc atgactggcc gacggacggc gccgcgaatc
     2041 ttcatcctcg cgtcgattct gaccgtggtg gtggccgtcg tcggcttcat cgtgacgatg
     2101 gtgctgaacg ccttcgtctt cgatgaatac gacgcctacg gcgaggtgcc gatcccgggt
     2161 tcgagcagcc tgcatcttcc tgcaggccag gcgacggtca gcttccacac ccagaccatc
     2221 ggcagtccca gcggcggcgg cctacccgtc ccgcagctgg gcctgaccat ctctccgccc
     2281 gagggggtag cggagccaga ggtcacccag agctggggca ccacaaccag cgtcaacaac
     2341 gacgcccaca tccgggtttg ggcggtccgg attccggccg agggcactta ccacgtcacc
     2401 accgacggca atgtcaacgg ctacatcaac ccgcaactgg cattcgggca cgactcctcc
     2461 aacagcacgc tgatgtgggc cttcgccggc ctcttcgtca tcggcctgct ccagctgacg
     2521 gcggcgataa cctatgcggt gaaaagcggt aaacgcccca caccggccgt ccccgtccag
     2581 tcgacgttcg atgtcggcga ctcgtacgga atcagggatc cgcataagtc tgccgacgac
     2641 gggattcggc tggagcagct cagaaccctg gcgggactgc gtgattccgg cgcgctgacc
     2701 gaagcggagt tccaggccga gaagcgccgc atcctcaaca cctaacgggc ttctccctca
     2761 ccgcgagcgt gcgtgtctgc tccgcgacac gcggcagatc gtgtgcaagc gtgcacgctc
     2821 gcggggtgag gggagctagc cgacggggtg acccgcggcg agtgccttga cgtcggcggt
     2881 gtgcagcgga gtaccgccga ccccccagtc gccactggcg atctcctcga ccacgaccca
     2941 agtgaccggc cgcatgttct cgccctcgat ctcgaccatg gcgttggtca cgctgcgtac
     3001 gatgtcctgc ttctgagccg ccgagaacac tccttcgatg accttcacgt tgatgaatgg
     3061 catcgtcttc tccttctctt tattctccag ccggccagcg gaattgctgg ccggaaccat
     3121 ctcgctgacc gacatcggga aggtctgcga agcgcggtgt attcggttcg agcggcagtc
     3181 gaagtccgtt gacgaggtag ctcaccatgc ggtaaaagcc ggcgagcatg agcaattcga
     3241 tgatcgcctc gtccgagtag taattggtga ggctgtccca caggtcgtcg tcgacagtgc
     3301 agaaatcgcg caagctgtcg cacagtgcga tcagaacccg gtcctcgtcg gcccaaaggt
     3361 cttcgctcgc atggtctctc accagtgatg cgatctgtcg atcgtccaac ccggccttgg
     3421 tggcgtaggc cgctacgtga acaccccact cgtactcggc accgcagtgt gccgtcacgc
     3481 ggtcgatcac gatctcgcgc tgccggatgt ccaggtgacc ccgatcgaga agcccggcgc
     3541 tgaacagctt gtagaagagc cgccgatcgc gggccaacgt ggtgaagaga accagcgggt
     3601 cgttgccccg catgatggca tctatggagt cttggatgtc ggccggaagc ggcgaggatg
     3661 ccggatcgac ccgtgcgcta ttaatcttat agcgtgtcat ggttgccacc gtacccacac
     3721 gtgctacaaa atgtaaagca tggatagtcc gaaggtcggt cgacccgcac gaggctcgtc
     3781 cacagggcga ccgatcatgg tcgttctcga cgtgctgggc cgtcgtggag cgctgcgtgt
     3841 gttatgggaa cttcgcga
//
LOCUS       DASYLX010000921         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1042723,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000921 DASYLX010000000
VERSION     DASYLX010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042723"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGP27_21525"
     CDS             <1..528
                     /locus_tag="VGP27_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEV7853911.1"
                     /translation="DDGLRILRLGVDTHLITSHCLHPLLIERPGGLLVEVTDGTRRYN
                     DENFRLSVFYDLAKSSVNRLAFAHGHELAGYGGTAVAVTPGWLRSEMMLDNWGVSEAN
                     WHEALNPNRGGGLPTAPPGFAESESPRFVGRGIAAIAADENRARWNQGSMTSADLARE
                     YGFTDIDGRQPDGWV"
     gene            577..1017
                     /locus_tag="VGP27_21530"
     CDS             577..1017
                     /locus_tag="VGP27_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HEV7853912.1"
                     /translation="MAVEETVRGLWKALSDRDWDSVTSFLSDDCLYVDMPMPAASARG
                     RENVITRLKIGLESLSSYVNHDGLLLSNGVDVIYEHSETWGWHSGETVRLPFVSVHRV
                     EDDEITLWKDYLDFSTLLSAAPPSYLETLASADTSWVYDASDLV"
     gene            complement(1027..>2188)
                     /locus_tag="VGP27_21535"
     CDS             complement(1027..>2188)
                     /locus_tag="VGP27_21535"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV7853913.1"
                     /translation="TGIASSGALGVVGDQRIRIDLGALRILGDGLAWAPGSFYDQDGG
                     PDPYCCRGALSRIERRLTDAGIDALVGHECEFVLVGPDGSRLPAHLWAQYGLAGVLEY
                     EGFVRDVTNAATSSGVSIEQFHPEYGLNQFEVSLTPQSPTAAADQLLLIRMIVGRVAR
                     RYGLRVSLSPVPFAGGVGSGAHQHFSLKKGDTPLFSGGSGACGMTPEGESAVAGVVAG
                     LPEAEGILCGSILSGLRMAPGHWSGAFVCWGTENREAAVRFLVGGNSNPLGANVEVKV
                     VDPSANPYLATATILGLALDGIERKLTLPPETAVDPSTLTDQQRDDAGVGLLLTSQAE
                     AIDLLDQSALIRGILGDPAVDATVAVRRYEHETYGHLSDEELTDRFRMAWSV"
BASE COUNT          368 a          714 c          732 g          374 t
ORIGIN      
        1 gacgacggac tgcggatcct caggctgggc gtcgacaccc acctcatcac ctcgcactgc
       61 ctgcatccgc tgctgatcga gcggccgggc gggttgctcg tcgaggtcac cgacggcacc
      121 cgccggtaca acgacgagaa cttccggctc tcggtgttct acgacctcgc caagagctcc
      181 gtcaaccggc tcgcgttcgc gcacggtcac gaacttgcgg ggtacggcgg caccgctgtc
      241 gccgtgacac ccggatggct gcgctcggag atgatgctgg acaactgggg tgtcagcgaa
      301 gccaactggc atgaggcgct gaacccgaac cgcggcggtg ggctcccgac cgcgccgccg
      361 gggttcgccg agtcggaatc gccgcggttc gtcggccggg gtatcgccgc gatcgccgcc
      421 gatgagaacc gcgctcggtg gaaccagggg tcgatgacgt cggcggacct tgcgcgcgaa
      481 tacggcttca ccgacatcga tggccgccaa cccgacggct gggtgtagcc ggtgatctca
      541 ttgacagccg aatgtcgcgg actgttctat gtctgggtgg ccgtcgagga gaccgtgcga
      601 ggtttgtgga aggcgttgtc ggatcgcgac tgggattcag tgacgagctt tctgtccgac
      661 gactgtctgt acgtcgacat gccgatgcct gcggcgtcgg cgcgcggccg tgagaacgtc
      721 atcacgcgac tgaagatcgg gctggagtcg ctgtcgtcgt acgtcaacca cgacgggctg
      781 ctgctgagca acggcgtcga cgtcatatac gagcactccg aaacatgggg ctggcacagc
      841 ggggaaaccg ttcggctgcc cttcgtatcg gtgcaccgtg tcgaggacga cgaaatcacc
      901 ctctggaagg actacttgga cttcagcacg ttgttgtctg cggcgccgcc gtcatatctg
      961 gaaaccctgg cgtcggctga cacgtcatgg gtgtacgacg ccagcgacct ggtctgagaa
     1021 cggttttcac acgctccagg ccatgcggaa cctgtcggta agctcttcgt cgctgaggtg
     1081 tccgtaggtc tcgtgctcat agcggcgcac ggccaccgtc gcgtcgaccg ccggatcgcc
     1141 gagaatgccc cggatgaggg cggattggtc gagcaggtca atggcctcgg cttgactggt
     1201 aagcagcagg ccgacgccgg cgtcgtcgcg ctgttggtcg gtcagggtgg acgggtcgac
     1261 ggccgtctcc ggcggcaagg tcagcttccg ctcgatgccg tcgagcgcga gcccgaggat
     1321 agtcgccgtc gcgaggtacg ggttcgccga tgggtcgacg accttgactt cgacgttggc
     1381 tcccaatggg ttgctattgc cgccgacgag gaatcgcact gcggcctcgc ggttttcggt
     1441 gccccagcag acaaaggctc cagaccagtg tccgggcgcc attctcagtc cggacaggat
     1501 cgagccgcac aggatgccct ccgcttcggg cagaccggcc accactccgg ctaccgcgga
     1561 ctccccttcc ggcgtcattc cacatgcacc ggatccgccc gagaacagcg gtgtatcgcc
     1621 cttcttgagc gagaagtgct gatgggcacc ggaacccacg cctccggcga acggcaccgg
     1681 cgacagactc acccgcaggc cgtaccgcct ggcgaccctg ccgacaatca tcctgatcag
     1741 cagcagttga tcggcggcgg cggtcggcga ctgcggagtc agcgatacct cgaactggtt
     1801 cagcccatac tcggggtgga actgctcgat ggagacacca gagctcgtgg ccgcgttggt
     1861 gacgtcgcga acgaaaccct cgtactcgag cacgcctgcc aggccgtact gcgcccacag
     1921 gtgggcgggc agccggctgc cgtccggccc gacgaggaca aactcacact cgtgcccgac
     1981 gagcgcgtcg atccccgcgt cggtgagcct gcgttcgatg cgggacagcg ccccgcggca
     2041 gcagtatggg tccggtccgc cgtcctggtc gtagaaagag cctggcgccc aggctaatcc
     2101 gtccccgagg atgcgcagcg cgccgagatc gatcctgatg cgctggtcgc cgacgacgcc
     2161 gagggcgcca ctcgacgcga tacctgtc
//
LOCUS       DASYLX010000922         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_384458,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000922 DASYLX010000000
VERSION     DASYLX010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384458"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGP27_21540"
     CDS             complement(<1..569)
                     /locus_tag="VGP27_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853914.1"
                     /translation="MTVDNEIRRRPEPVAAKVPEITASFWVIKLLTTAMGEAASDYLL
                     STMSFVGLGLGAAGFAFTLWFQLRTRRYNAFTYWAAVMMIAVFGTMAADTLHHQLGVS
                     FGTSTLVCALAVAATLWAWYRVEHTLSIHSITTRRREVFYWLTVSFTFALGTAAGDLT
                     ASQLHFGFVGSIVLFAVVMAVPALGWWRFR"
     gene            901..1695
                     /locus_tag="VGP27_21545"
     CDS             901..1695
                     /locus_tag="VGP27_21545"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEV7853915.1"
                     /translation="MASKAHGGETPTSPVRIDSKRIRQDESDLLASTTSGASSPDRQF
                     MRDVLLVALTVASGAVDAISYFGLGKIFSAFMTGNIVFLGFGIAETEGPDVIPVLVAL
                     SMYALGAYLGLRFTSRRTQEPGLWPPAMTVLLGLVAIAEAFFLVGWLAATDHPSAQVA
                     YVFIALLSLAMGIQTAAVRSLGVQGIFTTAGTFTLVAFVGTFTGSRSRAEMPRLAGVL
                     LGLIVGALAGGLLFLHARSYAPVLPLVLTILVLLVGRSMHSARRIA"
     gene            complement(1830..>3489)
                     /locus_tag="VGP27_21550"
     CDS             complement(1830..>3489)
                     /locus_tag="VGP27_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEV7853916.1"
                     /translation="GGRPPADVAGKTVILVDDGLATGASMLAAVQALREREPAQIVIA
                     VPAAPESTWREFAGLVDDVVCASMPHPFLAVGESYWEFSQVSDEEVRELLETPTTPAP
                     LTAVVESPADQVRRIAVDAPDGIPPLQVLDELIGDARVVLIGESSHGTHEFYKARAEI
                     TKWLIDRKGFLGVAAEADWPDAYRVNRFVRGIGQDETAEEALRGFERFPTWMWRNTVV
                     RDFADWLRDRNKRVVARRQAGFYGLDLYSLHRSMQAVITYLQKVDPEAAARARQRYSC
                     FDHASAADDGQAYGFAAAFGAGSSCEQQAVEQLMEFQQNSVEYARRDGLLAEDEAFYA
                     ERNARLVRDAEAYYRAMFSGRVNSWNLRDQHMADTLDALLEHLDHEDAEQSRIVVWAH
                     NSHVGDARATEVYADGQTTLGALARDLYPGKTRLIGFSTHTGTVTAASEWGGIAERKI
                     VRPALPNSVEELFHETDKDAFYVKMRDDGRPSVEPLDDIWLGRAIGVIYLPATERQSH
                     YFHVRPDDQFDAMIHIDNTRALEPLELTSEWTAGELHETYPTGL"
BASE COUNT          609 a         1128 c         1102 g          650 t
ORIGIN      
        1 cggaaacgcc accagcccaa cgccggaacg gccatgacga cggcgaacag cacaatcgat
       61 ccgacgaatc cgaagtgcag ttggctggcc gtaaggtcgc cggcggcggt tccgagggca
      121 aacgtaaacg acactgtcag ccaatagaac acctcgcggc gtcgtgtggt gatgctgtgt
      181 atagacagtg tgtgctcaac gcgataccac gcccacaagg tggccgcgac cgcgagggcg
      241 cagaccaggg tggacgtccc aaaggagacg ccgagctggt ggtggagggt gtcggcggcc
      301 atggtgccga acacggcgat catcatcacc gccgcccagt aggtgaaggc gttgtaccgt
      361 cgtgtccgaa gctgaaacca cagggtgaat gcgaaacctg ctgcgccaag accgaggccg
      421 acgaagctca tcgtgctgag caggtagtcg gatgccgctt cgcccatcgc ggtcgtcagc
      481 agcttgatga cccagaacga cgccgttatc tcgggcacct tcgccgcgac gggctcgggt
      541 cgtctgcgga tttcgttatc gactgtcatg ctttcctctc gctcgccggt tgggccacgg
      601 caggtacttt cagaattccg ttgcaccaaa ccgatgccat caccggtgac acgacttccg
      661 gccccccgtg agccagaacc tcatctgcct gccaccgtga aatccgactg gttcgcacca
      721 gttcgcgact gttcaagagt tgcgttgcag gaattggctt ttcgaaagga gtacgacggc
      781 cggctccgat tagcggcgtt gccacccgcg ctggcaggta cgtgacgctc cagctggcgg
      841 gggctgcaga tgcgggacag ccgtgacgac gtttcgacgc cgagacggca aactgtcgtc
      901 atggccagca aagcccacgg cggggagacg cctacatcgc cggtccggat agacagtaaa
      961 cgaattcgtc aagacgaatc agacctgctc gcgtcaacca cctccggagc gagcagtccg
     1021 gatcgccaat ttatgcgcga cgtgctcctc gtagcgctga cggtcgcttc cggagcggtc
     1081 gacgccatca gttatttcgg cctgggaaag atcttctccg cgttcatgac cggaaacatt
     1141 gtgttcctgg gattcgggat cgcggagact gagggccccg atgtcatacc cgtgcttgtc
     1201 gcgctgtcta tgtacgccct gggcgcctat ctcggcctgc ggtttacatc gcggcgtacc
     1261 caagaacccg gattgtggcc gcccgcaatg acggtgttgt taggtctcgt ggcgatcgcc
     1321 gaggccttct tccttgtcgg gtggttggca gctaccgacc acccctcggc ccaagttgcc
     1381 tacgtgttca tcgccctgtt gtcgctcgca atgggtatcc agaccgcggc ggttcgatcg
     1441 ctcggcgtgc aagggatatt cacgacagcg gggaccttta ccctcgtcgc atttgtgggc
     1501 accttcacag gatcccggtc acgggccgag atgccccgtc tagccggcgt gctcctgggg
     1561 ttgatcgtag gcgccctcgc gggcggactg ttattcctgc acgcacgcag ctacgccccc
     1621 gtgctgccgc tcgtgctgac cattctggtg ctcctggtgg gacggtccat gcatagtgcg
     1681 cggcgaatcg cgtgaaccaa attgaactgt cccgccgggc gcctcacacc tgcgcgcggt
     1741 ggtcgcggcg ccgaaatgat cggcacagca acagaacgcc agaccggtgg gagccggtga
     1801 cagctgcaac gcgccgtagc ttgcgcgcgc tagaggcccg tcggatacgt ctcgtgcagc
     1861 tcgccggcgg tccactcact ggtcagctcg agcggttcga gggcgcgcgt gttgtcaatg
     1921 tggatcatgg cgtcaaattg atcgtccggc cgtacgtgga agtagtggct ctgccgctct
     1981 gtcgcgggta gatagatcac cccgatcgca cgcccaagcc agatatcatc cagtggctcg
     2041 accgatggtc ggccgtcgtc gcgcatcttc acgtagaacg cgtccttgtc ggtttcgtgg
     2101 aacagttcct cgacgctgtt gggcagggcg ggtcggacga tcttgcgctc tgcgatcccg
     2161 ccccattcgc tggccgctgt cacggttccg gtgtgggtgc tgaaaccgat caggcgcgtc
     2221 ttgccggggt agagatcgcg cgccaatgcg cccaacgtgg tttggccgtc cgcgtagacc
     2281 tcggtcgccc gggcatcgcc gacgtgagag ttgtgcgccc acaccacgat ccgggactgt
     2341 tcggcatcct cgtgatccag gtgctcgagc aaggcgtcca aggtgtcggc catgtgctgg
     2401 tcgcgcaggt tccaggagtt gactcgaccg ctgaacatcg cgcggtagta ggcctcggcg
     2461 tcgcgaacga ggcgcgcgtt acgctcggcg tagaaggctt cgtcctcggc gagcagaccg
     2521 tcgcgccgcg cgtactccac cgagttctgc tggaactcca tcaattgctc gacggcctgc
     2581 tgctcgcacg acgaccccgc accgaatgcc gcagcgaagc cgtacgcctg tccgtcgtcg
     2641 gctgcggacg catggtcgaa gcacgaatag cgttgacggg cgcgggccgc cgcctcaggg
     2701 tccaccttct gcaggtaggt gatcaccgct tgcatcgacc gatgcaggct gtagaggtcc
     2761 agaccgtaaa aacccgcctg ccgccgcgcc accacccgct tattgcggtc acgcaaccaa
     2821 tcggcgaagt cgcgaacgac ggtgttgcgc cacatccagg tcggaaaacg ctcaaatccc
     2881 cgcagcgcct cctcggccgt ctcgtcctga ccgatgccgc gtacgaagcg attcacccgg
     2941 tacgcatcgg gccaatccgc ttccgccgcg actccgagga aacccttgcg gtcgatcaac
     3001 catttcgtga tctcggcgcg ggccttgtag aactcgtgcg tgccatggga actctcgccg
     3061 ataagtacca cccgggcgtc gcctatgagc tcatccagca cctgaagcgg cggaatgccg
     3121 tcgggcgcgt caacggcgat gcggcgaacc tgatcggccg ggctttcgac cactgccgtc
     3181 agcggggcgg gagttgtcgg ggtctcgagc agctcgcgca cctcctcgtc gctgacttgg
     3241 ctgaattccc agtaggattc gccgactgcg aggaacgggt ggggcattga cgcgcatacg
     3301 acgtcgtcga cgagtccagc gaactcccgc cacgtggatt ccggagctgc cggtaccgcg
     3361 ataacgatct gcgcgggttc cctctcacga agcgcctgca ccgcggccag catgctggct
     3421 ccggtggcca gtccgtcgtc gacgagaatc accgtcttgc cggccacgtc cgccggcggc
     3481 cggccaccg
//
LOCUS       DASYLX010000923         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3894850,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000923 DASYLX010000000
VERSION     DASYLX010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3894850"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1021
                     /locus_tag="VGP27_21555"
     CDS             17..1021
                     /locus_tag="VGP27_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7853917.1"
                     /translation="MPAVTADYLQLPRLDVPPLDAIERPVRSVTTAPTGLEGEGFPVH
                     RAFAGVSHAVLDPFVHMDQMGAVQYAPGEPKGTPWHPHRGFETVTYMIDGRMQHQDSH
                     GGGGMIEDGATQWMTAGSGILHIETPPEDLVMSGGLFHGVQLWVNLPAEKKMIPPAYQ
                     GIGAAAVKLAVSADAGALVRVIAGGVGGHRGPGSTHTPITLIHATVSPGARLQLPWDP
                     SFNALIYVLAGAGRVGTEGRQVRDGQLVVLGRGDWFEVRGNDLQGSQSPALELLVLGG
                     RPIREPVAAYGPFVMNTKAELGRAMEDFQAGFFGQIPVDALMPHVPDPAPIRTENTGI
                     "
     gene            1049..1222
                     /locus_tag="VGP27_21560"
     CDS             1049..1222
                     /locus_tag="VGP27_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853918.1"
                     /translation="MADSTWGFDFVNYMLAIRLSDGRRQAVPLAAKPVAEVDGETLSA
                     LASNHPSRASPTR"
     gene            1481..>2898
                     /locus_tag="VGP27_21565"
     CDS             1481..>2898
                     /locus_tag="VGP27_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7853919.1"
                     /translation="MGSSTGVFDVGGQRLVTPPIRGRVGELKVIGALVSALVQGRGGV
                     LVIEGPPGIGKSRLLTEVMALADKAGVRTLFGEAFEYQQMVPFFSLFMATLRADPPVG
                     DAEALRRLGGSVDLRYWVVRDLADAIHDAAAQTPLAIVLEDIHWADTGTLLALRSLAT
                     ARPDVAVLWVLTARTGAGGPLLQETLSVLQRADAAVLRVAPMSPDAVADVVQDAVRAN
                     ADESLLSLAAKAHGNPFLVKELVGGLDEEGRLNVSGGRAVAIGHALPRRLGASMQQRL
                     DHLSTGAGEVVRVAAVLPDRFSAGLLAAMLERQPSSLMAALEEAVRADLLVEDDEQLR
                     FRHDLLREATRQSLPQSMRRAMERQSASIMLSMGAAPAEVATQLAHSAEPGDREAIDA
                     LRQAAQSVGHSDAGAAADLSKRALELLTADDADHGLLVAETVELLNRASRYAEAEELA
                     VAALSAVASSEVEAEIRLRLPTL"
BASE COUNT          456 a          868 c         1051 g          523 t
ORIGIN      
        1 ctgatgggag ttgatcatgc ctgctgtaac ggccgactat ttacagttgc cccggcttga
       61 cgtgccgccg ctggatgcaa ttgagcgtcc tgtgcggagc gtgaccacgg cgccgacggg
      121 gcttgaaggc gaaggctttc ccgtccaccg cgcctttgcc ggggtgtcac acgcggtgct
      181 tgatccgttc gtgcacatgg atcagatggg cgcggtgcaa tacgcgcccg gtgagcccaa
      241 aggcacaccg tggcatccac atcgggggtt cgaaaccgtg acctacatga tcgacggtcg
      301 catgcagcac caggactcgc atggcggcgg ggggatgatc gaggacgggg ccacccaatg
      361 gatgacggcc ggttcgggga tcttgcacat cgagacgccg ccggaggact tggtgatgtc
      421 gggcggcctg ttccatggcg tgcagttgtg ggtgaatctg cccgccgaga agaaaatgat
      481 tccgccggcc taccagggca tcggcgccgc cgcggtgaaa ttggcggtgt ccgccgatgc
      541 gggcgcgttg gttcgggtga ttgcaggtgg ggtcggtggc caccgaggcc cgggatcgac
      601 gcacacgccg atcacgctga tccatgcaac cgtttcgccg ggcgcgcgat tgcaactgcc
      661 gtgggatccg tcctttaatg cgctcatcta cgttcttgct ggcgccgggc gcgtgggtac
      721 cgaggggcga caggtacgtg acggtcagct ggtggtgtta ggccgcggcg actggttcga
      781 ggtgcgcggt aatgacctcc aaggctcgca aagtccggcc ttggagctgc ttgtcttggg
      841 tggtcgcccc attcgcgaac ctgtcgcggc atacgggccc tttgtgatga acaccaaagc
      901 tgaactggga cgggccatgg aggacttcca ggccgggttt ttcggtcaga ttccggttga
      961 tgccctgatg ccacacgtgc ccgacccagc gccgattcgc accgaaaata ccggaatctg
     1021 aggcttcgac ggctcattcc atcccggaat ggctgattcg acgtggggtt tcgacttcgt
     1081 caactacatg cttgcgatcc ggctcagtga cggtcgccgg caggcggtgc cattggcggc
     1141 caaacccgtc gcagaggtcg acggagagac gctctcagcg ctcgcgtcga accacccatc
     1201 ccgggcaagc ccaacgaggt agcgcccgct gcgccgttcg ctgaggacta tcagcacgct
     1261 gatcacgacc gcgaaatggc gaacacgttc tggtagcaac ttgtgcaggc ggaccgggtc
     1321 atgacgaact tttgggcgcc gttcatcgga aaggccagcc ccggtccacg cgtacgccga
     1381 gcgatcgacc gcaaatcggg ccgacgacgt gggcggatgc ttacaatgat ctgcactgca
     1441 agcggcagtc cagcaaagca aatggtagac aggacacccc gtggggtctt caaccggcgt
     1501 ttttgacgtc ggtggccagc ggctcgtaac tccgccgatt cggggccggg tgggcgagtt
     1561 gaaggtgatc ggcgcgctgg tgtcggcgct ggtgcagggc cgtgggggcg tgctggtcat
     1621 cgaggggccg ccgggcatcg ggaagagtcg gttgctgacc gaggtgatgg cactcgccga
     1681 caaggccggg gtccggacct tgtttggtga ggcattcgag tatcagcaga tggtgccgtt
     1741 tttctcgttg ttcatggcca ccctgcgcgc cgaccctccg gttggggacg cggaggcgtt
     1801 gcgccggctg ggcggctcgg tggatctgcg ctactgggtg gtgcgcgatc tggccgacgc
     1861 gattcatgac gcggcggcac agaccccgtt ggcgatagtg ctggaggaca tccactgggc
     1921 cgataccggc accctgttgg cgctgcggtc gttggccact gcgcggccgg acgtggcggt
     1981 gctatgggtg ctgactgccc gcaccggggc cggcggtccg ctgcttcagg agaccttgtc
     2041 ggtgctgcaa cgcgcggatg ccgcagttct gcgggtggcc cctatgtcgc cggacgcggt
     2101 cgccgatgtg gtccaggatg cggtgcgggc caacgccgat gagtcgctgc tgagtctcgc
     2161 cgccaaggcg cacggaaatc ccttcctggt caaggagctg gtcgggggtc tggacgagga
     2221 gggccggctc aatgtcagcg gcgggcgtgc ggtggccatc ggacacgcgc tgccgcgacg
     2281 tcttggcgcg agtatgcagc agcgactcga ccacctctcc accggtgccg gcgaggtggt
     2341 gcgggtggcc gcggtgctgc cggaccggtt ctcggccggc ctgctggccg cgatgctgga
     2401 acgccaaccg tcgtcgttga tggcggcgct cgaggaggcg gtgcgcgcgg atctgctcgt
     2461 cgaagacgat gagcagctga ggtttcggca cgacttgctg cgcgaggcca cccgacagtc
     2521 gttgccgcag tcgatgcggc gtgcgatgga gcgccagtcg gcgtcgatca tgctgagcat
     2581 gggcgcggcc ccagccgagg tagcgacgca gcttgcgcac agcgccgagc ccggcgaccg
     2641 agaggcgatc gacgcgctgc gccaagccgc gcaatcagtt ggccacagtg atgccggtgc
     2701 ggccgcggac ttgagcaagc gcgcgttgga gctgttgacc gccgatgacg ccgaccacgg
     2761 gctcttggtg gccgaaacgg tggagttgct caaccgggcc agccgctacg ccgaggccga
     2821 agagttggcc gttgcggcgc tctcggcggt ggcgtcgtcc gaagtggagg ccgagattcg
     2881 tctgcgcctg cccacgct
//
LOCUS       DASYLX010000924         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1482048,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000924 DASYLX010000000
VERSION     DASYLX010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482048"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..357)
                     /locus_tag="VGP27_21570"
     CDS             complement(202..357)
                     /locus_tag="VGP27_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853920.1"
                     /translation="MDQRNDPPENPEDAREATEDQRELQDELEHRNDDPNAPGGHQTR
                     AQVADET"
     gene            complement(368..>1721)
                     /locus_tag="VGP27_21575"
     CDS             complement(368..>1721)
                     /locus_tag="VGP27_21575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7853921.1"
                     /translation="RLQEPHVIPVHDFGDIDGTLYIDMRLVDGASLKDELRVNGPLEP
                     RRAASIIAQVAAALDAAHADGLVHRDIKPENVLLTPDDFTYLVDFGIAHGDGDASVTS
                     TGLIIGSCAYMAAERFSGGQVGPAADIYSLTCLLYECLTGRAPFETGGDLRQLMSAHM
                     FSPPPRPSVMRAGITRAFDEVVARGMAKKPSDRFATAGELAKAATAAAGSALPPRPPL
                     PTPPPPTGTQQFSAIYEDPSGTGYTPYPPQVHTPAPTPPPGRKPRFGRTQVILAAATL
                     LLLGVALILASMLVFGGGDTGTTPQTPLALPPSASTATETVTPSTTAESTTSTTPTTT
                     SSTSSAGLAGMTGMDGQGFVGHTTRCADGNPVVAAIQTALSMAVICETSPGNYQYRGE
                     RLRDGANIQLNNAAPDGGGFDVTNPADGARYEIRPDRLRILSNGGVDSDEPALQYGTR
                     "
BASE COUNT          240 a          601 c          575 g          305 t
ORIGIN      
        1 gcgacgatgc gatcggtctt ggtgtcgaag gccctgtaga cctcgcccat tccgccccga
       61 ccgatcagct cctgcagctg atagtgcccg aatggcgccg tatccaccgc gtttactcct
      121 cggccggcga acccgctttg atgtgtcgct tgaagctaca ccaggtttgc cgaggttgtc
      181 cgcgtgtgcg acaacacggg gtcaggtctc gtcggccacc tgagctcggg tttgatggcc
      241 gccgggcgcg ttcggatcgt cattgcggtg ctcgagttcg tcctgcagct cccgctgatc
      301 ctcggtggct tccctcgcgt cctccggatt ttccggcgga tcgttgcgct ggtccatcgg
      361 aactccccta ccgcgtcccg tactgcagcg ccggctcgtc ggaatcgacg ccgccattgc
      421 tgaggatcct gagccggtcc ggccggatct cgtatcgggc gccgtcggcc ggattcgtca
      481 cgtcgaaccc gccgccgtca ggtgccgcat tgttcagctg aatattcgcg ccgtcgcgca
      541 ggcgttcacc cctgtactgg tagttccccg gcgacgtctc gcaaatcacc gccatggaca
      601 acgccgtctg gatcgctgcc accaccgggt tgccgtcggc gcagcgggtg gtgtgcccga
      661 cgaacccctg gccgtccatc cccgtcatgc ccgccagccc cgccgagctc gtcgacgacg
      721 ttgtcgtggg cgtggtcgat gtcgtcgatt cggccgtcgt cgagggcgtc accgtctcgg
      781 tcgcggtgct cgccgacggc ggcagcgcca acggcgtctg gggcgtcgta ccggtgtcgc
      841 cacccccgaa gacgagcatc gacgcgagaa tcagggcgac accgaggagc agaagcgtcg
      901 ccgccgccag gatcacctga gtgcggccga accgcggctt gcggcccggc ggcggcgtcg
      961 gcgcgggtgt gtggacctgc ggcggatacg gcgtgtatcc ggtccccgac gggtcttcgt
     1021 agatcgccga gaactgctgt gtgccggtcg gcgggggcgg tgtcggcagc gggggacgcg
     1081 gcggcagcgc cgaacccgcg gcggccgtcg ccgccttggc cagctcgcct gcggtcgcga
     1141 acctgtccga cggcttcttc gccatacccc gcgcgaccac ctcgtcgaac gcccgggtga
     1201 taccggcacg catgacactc ggccgtggcg gcggcgagaa catgtgcgcg ctcatcagct
     1261 gtcgcaaatc tccgccggtc tcgaaaggcg ccctgccggt taggcattcg tacagcaggc
     1321 aggtcagcga gtagatgtcg gctgcgggcc cgacttgccc gcccgaaaac cgttcggcgg
     1381 ccatgtaggc acacgacccg atgatcagcc ccgtcgaggt cacgctcgca tccccgtcac
     1441 cgtgcgcgat cccgaagtcg acgaggtagg tgaagtcgtc gggcgtgagc aggacgttct
     1501 ccggcttgat atcgcggtgc accaggccgt cggcgtgcgc ggcgtccaaa gccgccgcca
     1561 cctgggcgat gatcgacgcc gcgcgcctgg gctccagcgg gccgttcacc cgcagctcgt
     1621 ccttcaggct cgcaccgtcg accagccgca tgtcgatgta aagcgtgccg tcgatatcac
     1681 cgaagtcgtg caccgggatg acgtgcggtt cctgcagtcg c
//
LOCUS       DASYLX010000925         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2361031,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000925 DASYLX010000000
VERSION     DASYLX010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361031"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGP27_21580"
     CDS             complement(<1..591)
                     /locus_tag="VGP27_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV7853922.1"
                     /translation="MRRWVVVASAALIALAACSTGDDAVAQGGTFEFVAPGGKTDIFY
                     DPPESRGRPGTLAGPSLMDPAKTLSLDDFTGKVVVINVWGQWCGPCRTEIGELQKVYD
                     ATKADGVAFLGIDVRDNNRDAARDFVTDRKVAFPSIYDPSMRTMIAFGGKYPTTVIPS
                     TVVLDREHRVAAVFLRELLAGDLQPIVQRVAAEPAPQ"
     gene            complement(588..1196)
                     /locus_tag="VGP27_21585"
     CDS             complement(588..1196)
                     /locus_tag="VGP27_21585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7853923.1"
                     /translation="MTEKTIVHVMRHGEVYNPDKILYGRLPDYHLSERGRAQAQRVAD
                     WLAGRDIVYVVASPLERAQETATPIAERHGLPIDTDDDLIESSNIFQGQRVSPGDGAL
                     RDPRNWWHLRNPRTPSWGEPYEQIAERMTAALDRARVKAAGHEAVCVSHQLPVETLRR
                     AVTGKSLHHFPTRRLCNLASLTSFYFHGDTYVGWGYSELAGQ"
     gene            complement(1193..>1709)
                     /locus_tag="VGP27_21590"
     CDS             complement(1193..>1709)
                     /locus_tag="VGP27_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7853924.1"
                     /translation="LFTFGKVMSGGLPAAAFGGRTEVMERLAPLGPVYQAGTLSGNPV
                     AMAAGLATLRAADDDVYEKLDANADRLADMLSRALADAGVAHGISRAGNMFSVFFTDA
                     PVTDFASAKATETWRYPAFFHALLDAGIYPPCSAFETWFVSAALDDLAFDRIAAALPA
                     AARAAAEARPA"
BASE COUNT          272 a          583 c          588 g          266 t
ORIGIN      
        1 ctgcggagcc ggctcggcgg ccacccgctg cacgatgggt tgcagatcac cggcgagcaa
       61 ctcccgcagg aacaccgccg cgaccctgtg ctcgcggtcc agcacgacgg tggaggggat
      121 gacggtggtg gggtacttgc ccccgaaggc gatcatcgtg cgcatcgacg ggtcgtagat
      181 ggacgggaac gcgaccttgc ggtcggtcac gaagtcgcgg gccgcatcac ggttgttgtc
      241 gcgcacgtcg atgccgagga acgcgacgcc gtcggccttg gtggcgtcgt acaccttctg
      301 cagttcgccg atctccgtgc ggcacggtcc acaccactga ccccacacgt tgatgaccac
      361 caccttgccg gtgaagtcgt cgagcgacag ggtcttggcg gggtccatca agctgggtcc
      421 ggccaatgtg cctgggcggc cgcggctctc gggcgggtcg tagaagatgt cggtctttcc
      481 gccgggggcc acgaactcga aggtgccgcc ctgcgcgacg gcgtcgtcac cggtggagca
      541 tgcggcgagc gcgatcagcg ccgcactggc aacgacgacc caccgcctca ctgcccggcc
      601 agctccgaat agccccagcc gacgtaggtg tccccgtgga agtagaagga ggtcagcgag
      661 gcgagattgc acagccgacg agtcgggaag tgatgcaacg actttccggt caccgcccgc
      721 cgcagcgtct ccaccggaag ctggtggctt acgcacaccg cttcgtggcc cgcggccttg
      781 accctggcgc ggtccagcgc ggccgtcatc cgctcggcga tctgttcgta gggttcgccc
      841 cacgacggcg tacggggatt gcgcaggtgc caccagttgc ggggatcccg cagggcgccg
      901 tcgcctggag aaaccctctg accctgaaag atattggatg attcgatcag atcgtcgtcg
      961 gtgtcgatcg gcaacccgtg ccgctcggcg atcggggtcg ccgtctcctg cgcacgctcc
     1021 agcggcgagg cgacgacata gacgatgtcg cggcccgcca accagtcggc gacccgttgc
     1081 gcctgggccc ggccgcgttc cgacaggtgg taatcgggca ggcggccgta gaggatcttg
     1141 tccgggttgt acacctcgcc gtgccgcatg acatgcacga tcgtcttctc cgtcatgccg
     1201 gccgggcctc cgccgccgcc cgggcggcag ccggtagggc ggccgcgatg cggtcgaacg
     1261 ccaggtcgtc gagtgcggcc gagacgaacc acgtctcgaa tgcgctgcac ggcgggtaga
     1321 ttcccgcgtc gagcagcgca tggaagaacg ccgggtagcg ccacgtctcg gtggccttcg
     1381 ccgaggcgaa gtcggtaacc ggtgcgtcgg tgaagaagac gctgaacata tttccagccc
     1441 gcgaaatccc gtgtgccaca ccggcatcgg cgagtgcgcg cgacagcatg tcggccagcc
     1501 ggtcggcgtt ggcatccaac ttctcgtaca cgtcgtcgtc ggcggcgcgc agcgtggcga
     1561 gccccgccgc catcgcgaca gggttacccg acagcgtgcc cgcctgatac accggcccga
     1621 gcggggccag ccgctccatc acctcggtcc ggccgccgaa cgccgccgcg ggtagcccgc
     1681 cgctcatcac tttgccgaag gtgaacagg
//
LOCUS       DASYLX010000926         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3348061,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000926 DASYLX010000000
VERSION     DASYLX010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348061"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGP27_21595"
     CDS             <1..1068
                     /locus_tag="VGP27_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011596428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7853925.1"
                     /translation="AGVFGRDPTASTRMAERLGVPADRAYRDYAEMAEGESARTDGVD
                     VVTIATPNDSHFAIASEFLRRGISVVCEKPLTQDSASSAELVELAASSGAILAVPHCY
                     SAYAMVRQAARMVRDGDLGAIRFVNVEHASGWASTPLELNGHKQASWRTNPEIGGEAS
                     VVGDLGTHALHLLRYITGLGATRVSAELSTLVPGRRVFDNAAIRLSLSNGATATLWAS
                     MAATGHEHGLRIRVFGDLASLEWRHADPQHLQVRDQHGGTTTLAHGQSTLSDDAKRLT
                     RPGLGHPEGFLEAFANFYTDLAEQLRARRDGTPYAHRELSIPTGIDGLIGVQFVEAAA
                     ASHADAGNWVVPAFASSNAVV"
     gene            1070..2065
                     /locus_tag="VGP27_21600"
     CDS             1070..2065
                     /locus_tag="VGP27_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7853926.1"
                     /translation="MHRFALIGAGFIGGVHARSLAAHPQVDFAMVYDVDPARASTVAE
                     QHGARVATPDEVFDPTAVDAVLIASSTDTHAGHLRRAAAANIPVLCEKPIDLDFARAA
                     EVVQYANDQGILAMVDFNRRFDRDYAELRRVVATGGVGSVHLIQMTTRGPSLPPIDYI
                     AVSGGQMRDQTVHFFDLARWIAQEEPEAVFATGSALTEPKLSDYGDVDLSTVALRLPS
                     GALVQIDSARQIGYGYDERIEVLGSTGMVEARRHRAGAVSRYTKDQVIDDGLHNSWFE
                     RVAASYRTALDHFVDALEGRGVIAPNLDEALKAQAIAEAATLSLTTGQMEPIKYG"
     gene            2058..2900
                     /locus_tag="VGP27_21605"
     CDS             2058..2900
                     /locus_tag="VGP27_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV7853927.1"
                     /translation="MADLVATAWTSAGATSPMITPSTSPVPVSERVAAVADAGYCGLG
                     LIADDLTVVRDTIGYPALKDLMTDYGLTHIEIELIERWWIPRGEPGHTYDTRELLLDA
                     ADVLSPAFIKIGSENGPAIDEPRQLVAPLRDLADEAQAHGTRVAIETMPFSAITTVPM
                     GVEIIEAANHPAAGLVIDAWHVFRAPTTLTELHSILKPEHIFGVELNDAAPEVIGTLF
                     EDTVQRRLLCGEGGFDLPGLVTLLRDKGFDGPWGVEILSSSFRQLPVPQAVKLAADSA
                     RTVL"
     gene            complement(3097..>3975)
                     /locus_tag="VGP27_21610"
     CDS             complement(3097..>3975)
                     /locus_tag="VGP27_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HEV7853928.1"
                     /translation="AKGAATSSKGKGTMIIGVDTLPELPTDPGDRNRTSPFAFTGNRF
                     EFRAPGSGQTINVPMIILNTIMADSLDYMATVLEKAVGEGEDFDKAVQQLLTEIITEH
                     GAVVFNGDGYSENWQIEAAERGLPNLKTTIDAIPQLVTPDAVEVFEKYGVFNERELRS
                     REEVRYETYALTVSVEAKLTLELGSTVILPAAIRYQTELAQNVATLKAAGVEADTALL
                     AAVSKPISELSSALTALKTAMETHGGDSAILEAKHAQSLLPLMDTVRTAADTLEGVVA
                     DDLWPLPTYQEMLYIL"
BASE COUNT          655 a         1362 c         1303 g          655 t
ORIGIN      
        1 gccggtgtct tcgggcgaga cccgacagcg tcgacgagga tggccgaacg actcggcgta
       61 ccggctgacc gtgcgtaccg cgactatgcc gagatggcgg agggcgaatc cgcgcggacg
      121 gacggcgtcg acgtcgtcac catcgcgact ccgaacgaca gccacttcgc catcgcgagc
      181 gaatttctgc gcagaggcat ctcggtggtc tgcgagaagc cgctgacgca ggactccgca
      241 tcgtcggccg agctcgtcga gctcgccgcg tcgtccggcg cgatcctcgc tgtcccgcac
      301 tgctattcgg cctacgcaat ggtccggcag gcggcccgga tggttcgcga cggtgatctc
      361 ggcgcgatcc ggttcgtgaa cgtcgagcac gcctcgggtt gggcctcgac gccgctggag
      421 ctcaacggcc acaagcaggc gtcctggcgg acgaaccccg agatcggcgg cgaggcaagt
      481 gtggtcggtg acctcggcac acatgccctc cacctgctgc gctatatcac cggcttgggc
      541 gccacccgcg tctccgccga actgagcacc ctggtgccgg gccgccgggt tttcgacaac
      601 gccgccatcc ggttgtcgct gtccaacggg gcgaccgcga cgttgtgggc cagcatggcc
      661 gcgacgggac acgaacacgg tctgcgcatc cgcgtcttcg gcgacctggc cagcctggaa
      721 tggcggcacg cggatcccca gcacctgcaa gtgcgcgacc agcacggcgg cacaacgact
      781 ctcgcgcacg gccagagcac actgtccgac gacgccaagc ggctcacccg tcccgggctg
      841 gggcatcccg agggcttcct ggaggcgttc gccaacttct acaccgacct cgccgagcag
      901 ttacgcgcca gacgcgacgg cacgccgtat gcccaccgcg agctgtccat cccgacgggc
      961 atcgacggcc tgatcggcgt gcagttcgtc gaggccgccg ccgcatcgca cgccgatgcg
     1021 ggtaactggg tcgtgccggc cttcgcttcg tcgaatgcgg tggtgtgagg tgcaccgctt
     1081 tgctctgatc ggcgcaggct tcatcggggg cgtgcacgcg agaagccttg ccgcccatcc
     1141 gcaagtggac ttcgccatgg tgtacgacgt cgatccggcg cgggcgagta cggtcgccga
     1201 acaacacggc gcgcgcgtag cgacgcccga cgaggtgttc gacccgaccg ccgtcgacgc
     1261 ggtcctcatc gcgtcgtcca ccgacacaca tgccggccat ctgcggcgcg cggccgcggc
     1321 caacatccct gtgctgtgcg agaagccgat cgacctcgac ttcgcacgcg ccgccgaggt
     1381 ggttcagtac gccaacgacc aaggcattct cgccatggtg gacttcaacc gccgtttcga
     1441 ccgcgactac gccgagcttc gacgggtggt ggcgacgggc ggcgtcggca gcgtccacct
     1501 gatccagatg acgacgcgcg ggccgtcgtt gccgccgatc gactacatcg ccgtctccgg
     1561 cggccagatg cgcgaccaga cggtgcactt cttcgacctg gcgcggtgga tcgcccagga
     1621 ggaacccgaa gccgtattcg cgacgggttc ggcgctgacc gagcccaagc tgagtgacta
     1681 cggcgacgtc gacctgtcga cggtggcgct gcgcctaccc agcggcgcac tggtccagat
     1741 cgacagtgcc cgtcagatcg gctatggcta cgacgagcgc atcgaggtgc tcggctcgac
     1801 gggcatggtc gaggcgcgcc gccaccgggc gggcgcggtg tcccgctaca ccaaggacca
     1861 ggtcatcgac gacggcctgc acaacagttg gttcgaacgg gtcgccgcga gttaccgcac
     1921 cgcccttgac catttcgtcg acgcactgga gggacggggc gtgatcgcgc ccaacctcga
     1981 cgaggcgctg aaggcccagg cgatcgccga ggcggcgacg ctttcgttga cgaccggtca
     2041 gatggaaccg atcaaatatg gctgacctcg tcgccaccgc gtggaccagc gcaggcgcca
     2101 catcgccgat gatcacaccg tccaccagtc cggtcccggt ctcggaacgt gttgcggcgg
     2161 tggccgatgc gggttactgc ggcctcggct tgatcgccga cgacctgacc gtcgttcgcg
     2221 acaccatcgg ctatccggcg ctgaaagacc tgatgaccga ctacggcctg acgcacatcg
     2281 agatcgaact catcgagcgt tggtggatcc cacgcggcga accgggccac acatacgaca
     2341 cccgtgagtt gctgctggac gcagccgacg tgctgtcgcc ggcgttcatc aagatcggct
     2401 cggagaacgg gccggccatc gacgaaccac gccaactcgt ggcgccgttg cgcgacctgg
     2461 cagacgaggc gcaggcgcac ggcacccggg tggccatcga aaccatgccg ttctcggcga
     2521 taacaacagt gccgatgggc gtcgaaatca tcgaagcggc caaccacccc gcggccggct
     2581 tggtcatcga cgcatggcac gtcttccgcg cgccgacaac gttgaccgaa ctgcactcga
     2641 ttctgaagcc ggagcacatc ttcggtgtcg aattgaacga cgcggcaccc gaagtcatcg
     2701 gcacgctgtt cgaggacacc gtccagcgcc gcctcctgtg cggcgagggc ggcttcgacc
     2761 tgcccggctt ggtcactctt ctgcgggaca agggattcga cggcccgtgg ggcgtggaga
     2821 tcctgtcgtc gtcgttccgg cagcttccgg ttccacaggc ggtcaagctg gccgccgact
     2881 ccgcccgaac agtcctgtag ccgccgccgc cccgcgagcg cccgcagagt gacggaattt
     2941 cgcggcgtgt cgcgcgcaga cgcgcacgct cgcgcggcag gggcaagggc ggaggcggaa
     3001 atgagttagc cgccgagtgt gggggctgcg ggttaatagc acctgcgatt gcgcaggcgt
     3061 ttacccccac actcgacgac gtcgtcagtg agttcgtcag agaatataga gcatctcctg
     3121 gtatgtcggc agcggccaaa ggtcgtcagc gacaacacct tccagcgtgt ctgcggcagt
     3181 acgcaccgtg tccatcaacg gcagcaggct ctgcgcatgc ttggcctcga ggatggccga
     3241 atcgcccccg tgggtctcca tcgccgtctt gagcgccgtc agcgcgctgc tcagctccga
     3301 gatcggtttc gacacagccg ccaacaacgc ggtgtcggcc tccacccccg ccgccttgag
     3361 cgtggcgacg ttctgcgcca gctcggtctg gtaccggatc gccgcaggca ggatcaccgt
     3421 ggacccgagc tccagggtga gcttcgcctc gacgctgacg gtcagggcgt acgtctcgta
     3481 ccggacctct tcgcgactgc gcagttcacg ttcgttgaag acgccgtact tctcgaacac
     3541 ctcgaccgcg tccggtgtga ccagctgagg gatcgcgtcg atggtggtct tgaggttcgg
     3601 cagtccgcgt tcggcggcct cgatctgcca gttctccgag tagccgtcac cgttgaacac
     3661 caccgcgcca tgctcggtga tgatctccgt cagcagctgc tgcacggcct tgtcgaagtc
     3721 ctcgccctcg ccgacggcct tctccagcac cgtcgccatg tagtccagcg agtccgccat
     3781 gatcgtgttg aggatgatca tcggcacgtt gatcgtctga cccgagccgg gcgcacggaa
     3841 ctcgaagcgg ttgccggtga atgcgaaggg gctggtgcgg ttgcggtcac ccggatcggt
     3901 cggcagctcg ggaagcgtgt cgacgccgat gatcatcgtg cccttgccct ttgacgacgt
     3961 ggccgcaccc ttggc
//
LOCUS       DASYLX010000927         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2249385,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000927 DASYLX010000000
VERSION     DASYLX010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249385"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGP27_21615"
     CDS             complement(<1..752)
                     /locus_tag="VGP27_21615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7853929.1"
                     /translation="MDGRPTDAAGLRLLVVGASSGIGHAVAVSAAGLGAKVAVAARRM
                     DLLSKLADSVGGTAFELDVEDASAINTVVGDAAEALGGLDAVVFTSTVVPFAHIEDTD
                     VTTWVHAFAVNAVGANNVLRAALPHLSDNGVVLIASSHDVGRPRAGVSAYSASKAALD
                     EILHSWRSEHPELSIMRVGIGPTADTEILRGADRDLLSLLFRSWVEHGQLPAQMSALT
                     DVANTLVSLVVTAHANPSVVPEIVQLSPRTRPT"
     gene            complement(755..1510)
                     /locus_tag="VGP27_21620"
     CDS             complement(755..1510)
                     /locus_tag="VGP27_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV7853930.1"
                     /translation="MTDDDGFPATFRRRHIVRLAVFAALLLGLFYLVAVTRVIDVETV
                     RRTVAATGPAAPLTYVAVSSVLGAIFVPGPILAAGSGVLFGPVLGLFVTLGATVGTAV
                     ITGLIGRRAGRQSARALLGAERADRVDRLIRRRGLWAVVGQRFIPGISDALASYAFGA
                     FGVPLWQMAVGAFIGSVPRAFVYTALGASVGDPSSPLTYAAIAIWCVTAIIGAFATHR
                     GYRHWRGRHRQPTDGPGTVPPAKGDLTPKKEGQ"
BASE COUNT          268 a          547 c          526 g          205 t
ORIGIN      
        1 gtcggcctgg tgcgcggcga caactgcacg atctcgggca ccacgctcgg attcgcatga
       61 gccgtgacga ccagggacac caacgtgttg gcgacgtcgg tgagggccga catctgcgcg
      121 ggaagctggc cgtgctcgac ccacgaccgg aacagcagcg acagcagatc ccggtccgcg
      181 ccgcggagga tctcggtgtc ggccgtcggg ccgatgccga cgcgcatgat cgacagttcg
      241 ggatgctcac tgcgccacga gtgcaggatc tcgtccaacg cggccttgct cgcgctgtac
      301 gcggacaccc cggccctggg gcggccgacg tcgtggctcg acgcgatcag caccacaccg
      361 ttgtcggaca gatggggcag cgcggcccgc agcacgttgt tggccccgac cgcattgacg
      421 gcgaacgcgt gcacccacgt cgtcacgtcg gtgtcctcga tgtgggcgaa cggcacgacg
      481 gtgctggtga acaccacggc atcgaggccg ccgagcgctt cggcagcgtc accgacgacg
      541 gtgttgatcg ccgacgcgtc ctccacgtca agttcgaacg cggtgcctcc gacggagtcg
      601 gccagcttgc tcagcaggtc catccgccgc gccgccaccg ccaccttggc gccgagtccg
      661 gcggcgctta cggcgaccgc atggccgatg ccggacgagg cgccgaccac gaggaggcga
      721 aggcctgcgg cgtccgtcgg tcgaccgtcc attgtcactg cccttccttc ttgggtgtta
      781 ggtcaccctt cgctggtggg acagtacctg gtccgtcggt cggctgacga tgacgcccac
      841 gccaatgccg gtagccgcgg tgggtggcga acgcgccgat gatcgcggtc acgcaccaga
      901 tcgcgatggc cgcgtaggtc agcggcgacg acggatcgcc gactgacgcg ccgagagcgg
      961 tgtagacgaa cgcccgcggc accgacccga tgaacgcccc gacggccatc tgccacaacg
     1021 ggacgccgaa cgcgccgaag gcatacgagg cgagcgcatc cgaaatcccg gggatgaacc
     1081 gttgaccgac caccgcccac agcccgcgcc gccggatcag ccggtccacg cggtcggcgc
     1141 gctcggctcc caacagcgcc cgcgcgctct gcctgcctgc acgcctgccg atcaggccgg
     1201 tgatgaccgc cgttccgacg gtggccccaa gcgtcacgaa caatccaagg acaggtccga
     1261 acaacacgcc actgcccgcc gccaggatcg gacccggcac gaagatcgcg ccgagaaccg
     1321 acgacaccgc gacataggtg agcggcgcgg ccggacccgt cgcggcgacg gtgcgtcgga
     1381 cggtttccac gtcgatcacc cgagtgacgg cgaccagata gaagagcccg agcagcaacg
     1441 ccgcgaagac ggcgagccgc acgatgtggc gtcgtcgaaa ggtggcgggg aagccgtcgt
     1501 cgtcggtcat gtcagacgcg attcttccgc acgccgcgta gggtcc
//
LOCUS       DASYLX010000928         9950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1594085,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000928 DASYLX010000000
VERSION     DASYLX010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594085"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="sppA"
                     /locus_tag="VGP27_21625"
     CDS             <1..1522
                     /gene="sppA"
                     /locus_tag="VGP27_21625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEV7853931.1"
                     /translation="PGPVQELRDAIAAFSDVKPSLAWAETYPGTLSYYLASAFREIWM
                     QPSGTVGLVGFATNALFLRDALDKAGVEPQYITRGEYKVATNRFTQTRYTDAHREADN
                     RLLESLHDQVWRAIATCRQIDSAAIDEMANRAPLLRDEAVAGRLIDRVGFRDEAYARI
                     GELVGSPGNSPQTGDADNDPDAPPRLYLSRYAKASASRPGPPLPSIPGRKPDPTIAVV
                     TVDGMIVSGRGGPQVLPLGNSSAGGDTIAAALREAAADDSVSAIVLRVDSPGGSVTGS
                     ETIWREVNRIRSSDTPIVASMGAVAASGGYYVAMCADAIVANAGTITGSIGVLTGKMI
                     ARELKERLGVSSDSLRTNDNADVWSIDKPFTDEQRARIEAEADLSYRDFIERVAAGRE
                     LSFEDVEEVARGRVWTGADALERGLVDELGGLRTAIDRAKVLAGHEKDADVRIVSFPG
                     SSFRDFLRPKPSSQPAAASLPEAVGALLGRSVKEVFVQAERSLTGVSALWLGDYRF"
     gene            1727..3115
                     /locus_tag="VGP27_21630"
     CDS             1727..3115
                     /locus_tag="VGP27_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853932.1"
                     /translation="MGSPDQPPQSTVSTEDVPPDVLKKAVAASAIGNATEWFDYGIYA
                     YGVTYISAAIFPGNTESATLLALMTFAVSFLVRPLGGLVWGPLGDRIGRRSVLAITIL
                     MMSGATLCVGLIPSYAMIGFWAPVLMVLLRMIQGFSTGGEYGGAATFMAEYAPPRRRG
                     FFGSFLEFGTLAGFSLGALLMLGFSLVLNDDQMNTWGWRLPFLVAAPLGLVGVYLRSR
                     LEDTPIFRELAESGETEERTATQFKDLLGRYWRPILRLGGVVVALNVVNYTLLSYMPT
                     YLEQSIGLSATMSLLVPIIGMLAMMVFLPIAGGLSDRVGRKPMWWFSLIGLFIAGIPM
                     FMLMGTNVVGAVIGFAVLGLLYVPQLATISAMFPAMFPTHVRYAGFAIAYNVSTSIFG
                     GTAPAVNEWLVNKTGDNLVPAYYMMAACVIGAIALIKVPETTRCPMNGTEMPGTPEAP
                     PQLEYEKQPGAV"
     gene            complement(3195..4460)
                     /locus_tag="VGP27_21635"
     CDS             complement(3195..4460)
                     /locus_tag="VGP27_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7853933.1"
                     /translation="MAASAQLSQVLKQATGVIAARGEGVLLYDEDDRRYLDFTAGIGV
                     TSTGHCHPRVVEAAQRQVGTLIHGQYTTVMHRPLLALVDRLGEVLPQGLDRIFFANSG
                     SEAVEAALRLSRQATGRPNVIVFHGGFHGRTVAAASLTTSGTKFRSGFSPLMAGVHVA
                     PFPDPTHFGWPVEQATDFALGQLDYILQTLTSPADTAAFLVEPVLGEGGYVPANDRFF
                     AGLRKRADQHGILLVVDEVQTGFGRTGRFWGGEHFNAVPDVLITAKGLASGFPLSAIA
                     ASSELMAKAWPGSQGGTYGANAVACAAALATLDVIRDERLVDNSAQRGAQLKEALQLV
                     ADKHDQIADVRGLGLMIGNEFRDADGKPDPVTAAVAQQEAARRGLLLLTCGAWGQVVR
                     FIPALVVTGDQVDEAASIWADAVGAVVPG"
     gene            4542..6008
                     /locus_tag="VGP27_21640"
     CDS             4542..6008
                     /locus_tag="VGP27_21640"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV7853934.1"
                     /translation="MSEHDEARVVQAVEKRLFIDGKWLDASEGRTFDVVDPATGRILC
                     SVAEASPADGRAALDAAVAAQPDFAAVSPRERANILTRSYELLHERIDDLALLMTLEM
                     GKPVTEARGEIAYAAEFFRHFAEEAVRIDGGYQTAPAGGARFLIARQPVGPCLLITPW
                     NFPMAMGTRKLGPAIAAGCTCVIKPAPQTPLSMLALMDILAEAGLPAGVVNCITVTEA
                     GPVMEPLIRSGLARKLSFTGSTQVGRLLLEQCAEKVLRTSMELGGNAPFIVFDDADLD
                     EAVEGAIAAKMRNMGEACTAANRMYVQSSVIDEFGRRLAARMASLSVGRGTNDGVAVG
                     PLIDKAALNKVETLVADAVGRGATVLTGGSAPGGDGFFYPPTVLTGVPRDAEMCDQEI
                     FGPVAPLMAFETEAEAVAAANDTEYGLVSYVFTNDLRRALRVAEALESGMVGLNQGVV
                     SNPAAPFGGIKQSGLGREGGLVGIDEFLETKYIGIAMR"
     gene            complement(6014..6691)
                     /locus_tag="VGP27_21645"
     CDS             complement(6014..6691)
                     /locus_tag="VGP27_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014361611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7853935.1"
                     /translation="MSSFIGPLVQESTPSIIAEKLRQAIAHGELKPGAQLGEAELARK
                     LGVSRGPLREGMQRLTQEGLLVSIRNRGLFVIDMTPDDVRDMYSAREAIERAAARRIL
                     DGDHRTAGKELLGIVDQMAEAETPADVSELDIAFHERLLKLAKSPRLSRMHQTFITET
                     RMCIHALDETYARSEVRDKEHRALANAIRKGDRELVDRLLAAHMDDAVNRLTLEKAVD
                     GAEHVGI"
     gene            complement(6688..7407)
                     /locus_tag="VGP27_21650"
     CDS             complement(6688..7407)
                     /locus_tag="VGP27_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase"
                     /protein_id="HEV7853936.1"
                     /translation="MPTVGLLYPGHSAEDDFAALEARIDGAVCLPVAITSVGEDAHRV
                     DALLDLGGQARLAEGAALLAVEKPDAVMWACTSGSFVFGPQGASAQASAIEAAAGVPA
                     SSTSIAFVDALRHLGIGRVAVAASYPDDVAQHFVAFLTAGGAEVVSMGSHGIYTAAEV
                     GTLRPEQVLAMVAAADHPKAEAVLVPDTAMHTLAIVDKLEAAVGKPVLTANQVTVWKG
                     LELLGSPPTLPGLGALFEARR"
     gene            complement(7537..8784)
                     /locus_tag="VGP27_21655"
     CDS             complement(7537..8784)
                     /locus_tag="VGP27_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV7853937.1"
                     /translation="MGVSAAEKSVLDKIDEHQLVELTRSLVRADGQNPPGEEAATVAV
                     LAAAAAELGLAVHEEPVAPGRNNLRVTLRTRGAGQAGRTRGAGQEGPPGEGPGLLLLG
                     HTDVVPVGEAWTRDPFGAAVEGGRIYGRGATDMKGGLAACLSAMAALRGAGLSGPVEL
                     AALVDEEENGTGIRAYVSSVQASFAGCITAEPTELQTIVGARGASYLRVDVHGTACHA
                     GNPGDGANAIYGAAAVVAEIERMHAQLADHPHPLLGPATWSVGQINGGNSGSVVPAEC
                     AVVADRRILPSESAATVLDDLRARIGALRLADRGLTVEVSMPMEMPAFETASESPLAR
                     AVGAAVADAGGPTLPPGGWTAACDGGFVARDLGVPVVVLGPGSVAEQAHRPDESVAIE
                     DLIVAARTYALTALRLLAHDKSA"
     gene            complement(8790..9947)
                     /locus_tag="VGP27_21660"
     CDS             complement(8790..9947)
                     /locus_tag="VGP27_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV7853938.1"
                     /translation="MVGSVIDSSTSLLQKQSHDVVRLAMGSPAAEAVPSEILAGIAAV
                     ELRSPDAYDYAATEGDPPLHAALLEMLRGTSDETTAERLTITAGGMQGLDLFCKIFVD
                     PGDLVTVESPTYTNGSATALSYGATLLEVPVDDDGMDIVELERLVGEAGRTPKAIYTI
                     PTFQNPSGVTLSLERRLRLLELAREWGSMVIDDDPYGLLRFAGEPLPTLRELGGGDPL
                     VFAVRTFSKIVAPGLRVGWVDTVPELQPLLINAKQAMDTCTNLPAQRLLAGFLTGGHL
                     DHHLVTQRAEYRRRKEAMHAALREHFSDRARWTDPQGGFFCWVTLDNVDSAALFEPAL
                     AEGVAFIPGSAFSPSGRFGDALRLCFASTPPDRIHEGVARLRRAVDLLAKA"
BASE COUNT         1607 a         3315 c         3356 g         1672 t
ORIGIN      
        1 gcccgggccc gttcaggaac tgcgggacgc gatcgccgcc ttcagtgacg tcaagccgtc
       61 gctggcgtgg gccgagacct atcccggcac gctgtcgtac tacctggctt cggcattccg
      121 tgagatttgg atgcagccgt ccggaacggt cgggctggtc ggcttcgcga ccaacgcgct
      181 gttcctccgc gacgcgctcg acaaggccgg cgtagagccg caatacatca ctcgcggcga
      241 atacaaggtc gccacgaatc gtttcacgca gacccggtat accgatgccc accgcgaggc
      301 ggacaaccgc ctgctggaaa gcctgcacga tcaggtgtgg cgggcgatcg cgacgtgcag
      361 gcaaatcgat tcggccgcca tcgacgagat ggcgaatcgg gcaccgctgc tgcgcgacga
      421 ggctgtggcc ggacgcctga tcgaccgggt cgggttccgc gacgaggcgt acgcgcgtat
      481 cggcgaactc gttggatcac cgggtaattc accgcagacc ggtgacgccg ataatgatcc
      541 cgacgcaccg ccgcggctct acttgtcgcg ttatgccaag gccagcgcga gccggccagg
      601 tccgccgctg ccgtcgatcc ccggccgcaa gcccgatccg acgatcgccg tcgtcaccgt
      661 ggacggcatg atcgtcagtg gccgcggcgg cccgcaggtg ttgccgctcg gaaactcgag
      721 cgcaggcgga gataccatcg ccgccgccct ccgcgaggcg gccgccgacg actccgtgtc
      781 ggcaatcgtg ctgagggtcg acagccccgg cggctctgtc accggatcgg aaaccatttg
      841 gcgggaagtg aatcggatcc gaagcagcga cacgccgatt gttgcatcga tgggtgcggt
      901 ggccgcctcc ggcggctact acgtcgcaat gtgcgccgat gcgatcgtcg ccaatgccgg
      961 cacgatcaca ggatccatcg gagtactgac cggcaagatg atagcccgtg agctcaaaga
     1021 gcggctcggt gtcagctcag attcgttgcg caccaacgac aacgcagacg tgtggtccat
     1081 cgacaagccg ttcaccgatg agcagcgcgc tcgtatcgag gcagaggccg acctcagcta
     1141 tcgagacttc atcgagcgcg tcgccgcggg ccgcgagctc agcttcgagg atgtcgagga
     1201 ggtggcccgc ggcagggtgt ggaccggtgc cgacgcgctc gagcggggcc tggtcgacga
     1261 actcggcggg ctgcggaccg cgatcgaccg ggccaaggta ctggccggtc acgagaaaga
     1321 cgccgacgtc cgcattgtca gcttccccgg ctcttcgttc cgggacttcc tccggcccaa
     1381 gccgtcgtcg cagccggctg cggcatcgct gcccgaggcc gtcggcgcat tgctcggccg
     1441 atcagtcaag gaggtgttcg tccaggccga gcggtcgttg accggcgtca gcgcgttgtg
     1501 gctgggcgac taccgcttct agcagcccag gcccccgttt ggcgaccagc tgagctgggt
     1561 agttcatttt cgtcatctct tgatgttcgt gatcgacttt gattagtgtc ggattgtcaa
     1621 caaaactacg acctgacccc gaggtcgtgc acaccctgcc cgtcgtgagt gtgggagccg
     1681 gcccggggaa gtgtccgaca gtgtcattga cacgaggagg ctcccgatgg gcagccctga
     1741 ccaacctcca caatcgaccg tcagcaccga agacgtaccg cccgatgtgc tgaagaaggc
     1801 cgtcgcggcc tcggccatcg gcaacgcgac ggaatggttc gactacggga tctacgcata
     1861 cggcgtcacc tacatctcgg cggcgatttt ccccgggaac accgagagcg cgacgttgtt
     1921 ggcgttgatg acgttcgcgg tgtcgttcct cgtcaggcct ctcggcgggc tggtgtgggg
     1981 tccgctgggc gaccggatcg ggcgtcggtc ggtgctggcc atcacgatcc tgatgatgtc
     2041 cggcgccacg ctgtgcgttg gtctcatccc gtcctacgcg atgatcggat tctgggcacc
     2101 ggtgctgatg gtgctgctgc gcatgatcca gggcttctcg accggcggcg agtacggcgg
     2161 tgccgccacc ttcatggcgg aatacgctcc gccccgcagg cgcggattct tcggcagctt
     2221 cctggagttc ggcacgctgg cgggcttctc gctcggtgca ctgctgatgc tcgggttctc
     2281 gttggtgctc aacgacgatc agatgaacac ctgggggtgg cggctgccct tcctcgtcgc
     2341 agcccctctc ggcctggtcg gtgtctacct gcggtcgcgg ctggaggaca caccgatctt
     2401 ccgcgaactg gcagagtccg gtgaaacgga ggagcgcacc gccacccagt tcaaggatct
     2461 gctcggccgt tactggcggc cgatcctgcg gttgggcggc gttgtggtcg cgctcaacgt
     2521 ggtgaactac accctgctga gctatatgcc gacctacctc gagcagtcga taggcctgtc
     2581 cgcaaccatg tcattgttgg tgccgatcat cggcatgctc gcgatgatgg tgttcctgcc
     2641 gatcgcaggc ggcctatcgg atcgcgttgg gcgcaaaccg atgtggtggt tctcgttgat
     2701 cggcctcttc atcgcaggca tcccaatgtt catgttgatg ggcacgaacg tggtcggcgc
     2761 ggtgatcggt ttcgcggttc tcggcctgct gtacgtgccg caactagcca cgatttcggc
     2821 gatgttcccg gccatgttcc ccacccatgt gcgctacgcc ggattcgcca tcgcgtacaa
     2881 cgtgtcgacg tcgatcttcg gcggcaccgc gcctgcggtg aatgaatggc tcgtcaacaa
     2941 gaccggcgac aatctggtgc ccgcctatta catgatggcc gcgtgcgtca tcggtgcgat
     3001 cgccctgatc aaggtgcccg agacgacgcg ctgcccgatg aacggaacgg agatgcctgg
     3061 caccccggag gcgccgccgc agctcgagta cgagaagcag cccggcgccg tctgagaggt
     3121 tcgcgcgaac gttccgtacc ggttcaaagt tcggccaact atcgagggtt gggaacgttc
     3181 gcgcgggctc gggctcagcc cgggacgacg gcgccgacgg cgtcggccca gatgctcgcg
     3241 gcttcgtcga cctgatcacc ggtcaccacc agtgccggga tgaagcgcac gacctggccc
     3301 cacgcgccac aggtcagcaa cagcaggccg cggcgagccg cctcctgctg ggcgacggcg
     3361 gcggtgaccg gatcgggctt tccgtcggcg tcacggaact cgttgccgat catcagcccg
     3421 aggccgcgta cgtcggcgat ctggtcgtgc ttgtcggcca ccagttgcag cgcctccttc
     3481 agctgcgccc cacgctgcgc tgagttgtcg acgagccgtt catcgcggat tacgtcgagg
     3541 gtcgccagcg cggcagcaca ggcaaccgcg ttggcgccgt aggtgccgcc ctgggacccc
     3601 ggccatgcct tggccatcag ctcagacgac gcggcgattg ccgacagcgg gaatccggag
     3661 gcaaggccct tggcggtgat gaggacatcg ggtactgcgt tgaaatgctc accgccccag
     3721 aaccttccgg ttcgtccgaa gccggtctgc acctcgtcga ccaccagcag gatgccgtgc
     3781 tggtcggcgc gtttccgcag tccggcgaag aaccggtcgt tggcgggcac atagccgccc
     3841 tcgccgagca ccggctcgac gaggaacgcg gcggtgtcgg cgggcgatgt cagcgtctgc
     3901 aggatgtagt cgagctggcc gagcgcgaaa tcggtggcct gctcgaccgg ccagccgaag
     3961 tgggtgggat cggggaacgg tgcgacgtgc acgcctgcca tcaacggaga gaagccggaa
     4021 cggaacttcg ttccggacgt ggtcagtgaa gcggccgcca ccgtgcggcc gtggaagccg
     4081 ccgtggaaga cgatgacatt cggccgccct gtcgcctgcc ggctcaatcg cagcgccgct
     4141 tcgacggcct cactgcccga gttcgcgaag aagatgcggt ccaagccctg cgggagcact
     4201 tcgcccagcc ggtcaacgag cgccagcagg gggcggtgca tgacggtggt gtactggccg
     4261 tggatgagcg tgccgacctg gcgttgtgcg gcctcgacca ctcgagggtg acagtgcccg
     4321 gtactggtga cgccgatgcc cgcggtgaag tcgaggtatc tgcggtcgtc ttcgtcgtac
     4381 agcagcacac cctctccgcg ggcagcgatg acgccggtcg cttgtttgag gacctgtgag
     4441 agctgtgcgg acgcggccac gcttctttcc cttgcctgtt gattggtaga ttgtcaacaa
     4501 tcagcgtatc ggacggaccg gcgtaaggcg aaggggtagc ggtgagcgag cacgacgagg
     4561 cgagggtcgt ccaggccgtc gagaagcgac tgttcatcga cggcaagtgg ctcgacgcat
     4621 ccgagggacg gaccttcgac gtcgtcgatc ccgccaccgg aaggatcctc tgttcggtcg
     4681 ccgaggcgtc cccggcagac ggcagggccg cactcgacgc ggccgtcgcc gcccagccgg
     4741 acttcgcggc ggtttcacca cgcgagcgcg caaacatact gacccgctct tacgagctgc
     4801 tgcacgagcg gatcgacgac cttgcgctgt tgatgacgtt ggagatgggc aaaccggtca
     4861 ccgaagcacg aggggagatc gcctatgccg cagaattctt ccgccacttc gccgaagagg
     4921 ccgtgcgcat cgacggcggt taccagacgg cgcctgcagg cggagcgcgc ttcctgatcg
     4981 cgcgccaacc cgtcggcccg tgcctgctga tcacgccgtg gaatttcccg atggcgatgg
     5041 gcacccgcaa gctgggcccg gccatcgccg ccgggtgcac ctgtgtgatc aagcccgctc
     5101 ctcagacacc gctgtcgatg ctggcgctga tggacatcct ggccgaggcc gggctgcccg
     5161 cgggcgtcgt caactgcatc accgtcacgg aagccggccc ggtcatggag ccgctgatcc
     5221 ggtcgggcct ggcccggaaa ctctccttca ccggctccac ccaggtgggg cggctgctgc
     5281 tggaacagtg cgccgagaag gtcctgcgga cctcgatgga actcggcggg aacgcgccgt
     5341 tcatcgtgtt cgacgacgcc gacctcgacg aggcggtcga gggtgcgatc gcggccaaga
     5401 tgcggaacat gggcgaggcg tgcaccgcgg ccaacaggat gtacgtgcaa tcttcggtca
     5461 tcgatgaatt cggacgccgg ctggccgcgc ggatggcttc gttgtcggtg ggccgtggca
     5521 ccaacgacgg tgtcgccgtc ggtcccctca tcgataaggc cgcgctcaac aaggtcgaga
     5581 cgctcgtcgc cgatgccgtc gggcgcggtg cgaccgtcct caccggggga tcggcacccg
     5641 gtggcgatgg cttcttctat ccgcccaccg tgctcaccgg tgtgccgcgc gacgccgaga
     5701 tgtgcgatca ggagatcttc ggtccggtgg cgccgctgat ggccttcgag acggaggccg
     5761 aggcggtcgc ggccgccaac gacaccgaat acggccttgt ctcttacgtt ttcaccaacg
     5821 acctgaggcg tgcattgcgg gttgcggaag ccttggagag cggcatggtc gggctcaacc
     5881 agggtgtggt gtccaatccg gcagcgccgt tcggcggcat caagcagtcg ggtctgggcc
     5941 gcgaaggtgg cttggtcgga atcgacgagt tcctcgagac caaatacatc ggcatagcga
     6001 tgcggtgacg gccctagatg ccgacgtgtt cggctccgtc cactgccttc tcgagcgtga
     6061 gccgattgac ggcatcgtcc atgtgggcgg ccagcaaccg gtcgacgagt tcgcggtcgc
     6121 ccttgcggat cgcgttcgcc agcgcccggt gctccttgtc gcgaacctcg gatctggcgt
     6181 aggtctcgtc gagggcatgg atgcacatcc gggtttcggt gatgaaggtc tggtgcatcc
     6241 gggagagccg cgggctcttg gcgagcttca acagccgctc atggaaggcg atatccagct
     6301 cgctgacatc ggcgggagtc tctgcttcgg ccatctggtc gacgatgccc agcagctcct
     6361 tgcccgccgt ccggtgatca ccgtcgagga ttctgcgggc ggccgctcgt tcgatcgctt
     6421 cccgcgcgct gtacatgtcg cggacgtcgt cgggggtcat gtcgatcacg aacaacccgc
     6481 ggttgcggat cgacaccagg agtccctcct gggtgaggcg ctgcatgccc tcccgcaacg
     6541 ggccgcggct gactccgagc ttgcgggcca actcggcttc gcccaactgt gcgccgggct
     6601 tcagctcgcc gtgagcgatc gcctggcgca gcttctcggc gatgatgctg ggcgtcgact
     6661 cctgaacgag aggcccgatg aagctgctca tcggcgcgcc tcgaacagcg cgcccagccc
     6721 gggaagcgtc ggtggggagc cgagcagttc gagtcctttc cacaccgtga cttggttcgc
     6781 cgtgagcaca ggcttgccga cggccgcttc cagcttgtcg acgatcgcca gcgtgtgcat
     6841 cgcggtgtcc gggaccagca ctgcctccgc cttcggatga tcggcggcag ccaccatcgc
     6901 gagcacctgc tcggggcgaa gagtgcccac ctcggctgcg gtgtaaatgc cgtggctacc
     6961 catcgagaca acctcggcgc cgcctgcggt caggaaggcg acgaagtgct gcgctacgtc
     7021 gtcggggtag gacgccgcga ccgcgacccg gccgatgccg agatgccgca gtgcgtcgac
     7081 gaacgcgatc gaggtcgacg acgcggggac cccggccgcg gcctcgatgg ccgacgcctg
     7141 cgccgacgcg ccctgcggac cgaacacgaa gctgcccgag gtgcacgccc acatcacggc
     7201 gtccggcttc tcgacggcca acagggccgc gccttcggcg agccgtgctt ggccgccgag
     7261 gtcgagcagg gcatcgacac gatgtgcgtc ctcgccgacc gacgtgatcg ccaccggcaa
     7321 acacacggcg ccgtcgatgc gcgcctcgag ggcggcaaag tcgtcctcgg cactgtggcc
     7381 ggggtagagg aggccaaccg ttggcatgtc atctcccttg gatcacgctg agcggaatag
     7441 gtagattgtt gacaatgtta acccgcgcta gctgcgacgc gggacacctc gccgaactgg
     7501 tcaccgacgc ttcatctcgg taactccctc cgtcgttcac gcggacttgt catgggccaa
     7561 cagccgtagc gccgtcagcg cgtaggtcct ggccgcaacg atcagatcct cgatcgccac
     7621 tgactcgtcg ggacggtgcg cctgctccgc gaccgaaccc ggcccgagga cgacgacagg
     7681 cacgccgagg tcgcgggcga cgaacccgcc gtcacacgca gcggtccaac cgccgggcgg
     7741 caaggtaggc cctcccgcat cggcgaccgc cgcgccgacg gcgcgcgcca gcggcgactc
     7801 cgacgccgtc tcgaaggccg gcatctccat cggcatcgag acctccacgg tcaagccgcg
     7861 gtcggccaga cgcagcgccc cgatccgcgc acgcaggtcg tcgagcacag tcgcggccga
     7921 ctcgctcggc aggatccggc gatcggcgac cacagcgcat tctgcaggca ccacgctgcc
     7981 gctgttccca ccattgatct gaccgacgct ccacgtcgcg ggacccagca aggggtgggg
     8041 atggtccgcc agttgggcgt gcatgcgttc gatctcggcc accacggcgg ccgcgccgta
     8101 gattgcgttg gcgccatcgc cgggattgcc tgcgtgacaa gcagttccgt gaacgtcgac
     8161 ccgcaggtac gaggctccgc gcgcgccgac gatggtctgc agctcggtgg gctcggcggt
     8221 gatgcatccg gcgaacgacg cctgtaccga ggacacatag gcgcggatac cggtcccgtt
     8281 ctcctcttcg tccaccagtg cagccagttc gaccggaccg ctcaaaccgg cgccgcgaag
     8341 tgccgccatc gccgaaaggc acgctgccaa accacctttc atatcggtgg caccacgccc
     8401 gtatatgcgg ccgccctcga cggcagcgcc gaacgggtcc ctggtccagg cctcgccgac
     8461 gggaacgacg tcggtgtggc cgagcagcag aagaccaggg ccctcgcctg gtggcccctc
     8521 ctgtcccgct cctcgcgtgc gccctgcctg tcccgctcct cgcgtgcgca gtgtgacgcg
     8581 caggttgttg cggcccggcg ccaccggctc ctcatgaacg gcgagcccca gttccgcggc
     8641 ggctgcggcg agtacggcga ccgtcgccgc ctcttcaccc ggcgggttct gcccgtcggc
     8701 ccgcaccaac gaccgggtca gctcgaccag ctgatgctcg tcgatcttgt ccagcacgga
     8761 cttctcggcg gcggacacgc ccacgccggt caggccttcg cgagcagatc gacggcgcgg
     8821 cgcagccggg cgacgccctc gtgaattcgg tcgggcggtg tcgacgcgaa acagaggcgc
     8881 agggcatcac cgaaccggcc ggacggtgag aacgccgagc cggggatgaa cgcgacgcct
     8941 tcggcaaggg caggctcgaa aagcgctgcg ctgtcgacat tgtcgagagt gacccagcag
     9001 aagaagccgc cttgtggatc agtccaccgg gcgcggtcgc tgaagtgttc gcgcagcgcc
     9061 gcgtgcatcg cctccttgcg ccgccggtac tcggcgcgct gggtcaccag atggtggtcg
     9121 agatgtccgc cggtgagaaa gcccgccagc aggcgttgcg caggcaggtt agtgcacgtg
     9181 tccatcgcct gcttggcgtt gatcagcagc ggctgcagct cgggaacggt gtcgacccag
     9241 ccgacacgca gccccggcgc gacgatcttc gagaaggtgc ggaccgcgaa caccagcggg
     9301 tcgccacctc cgagttcgcg cagcgtcggc agtggctcgc ccgcgaaccg caacaaaccg
     9361 taggggtcgt cgtcgatcac catcgacccc cattcacggg caagctcgag gagccgcagc
     9421 cggcgctcca acgacagggt cacccccgaa gggttctgga aggtcgggat cgtatagatc
     9481 gccttggggg tgcgcccggc ttcgccgacc aggcgctcga gctcgacgat gtccatcccg
     9541 tcgtcgtcga ccgggacctc cagcagtgtc gcgccgtagg acagagcggt cgcgctgccg
     9601 ttggtgtagg tgggcgattc gacggtgacg agatcgcctg ggtcgacgaa gatcttgcag
     9661 aacagatcga gaccctgcat gccgcccgcc gtgatcgtca ggcgctcggc agtggtctca
     9721 tcgctggtcc cgcgcagcat ctccagcagc gcggcgtgca gcggcggatc gccttccgtc
     9781 gcggcatagt cataggcgtc gggggacctc agttcgacgg ccgcgatgcc ggccaggatc
     9841 tccgacggca cggcctcggc cgccggactg cccatcgcga gccggaccac gtcgtgcgac
     9901 tgcttctgca gcagtgacgt gctcgagtcg atgaccgatc cgaccagccc
//
LOCUS       DASYLX010000929         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2361489,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000929 DASYLX010000000
VERSION     DASYLX010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361489"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VGP27_21665"
     CDS             <1..1318
                     /locus_tag="VGP27_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV7853939.1"
                     /translation="VEAARIGATGWQVTRTGARVVTKLAGRGSLQQKVIREIPQTFAD
                     LGPTYVKFGQIIASSPGAFGEQLSREFRSLLDRVPPADSGEIHKLFAEELGDKPENLF
                     NSFDEEPFASASIAQVHYATLHSGEEVVVKIQRPGIRRRVAADLQILKRGAQLVELAK
                     LGRRLSAQDVVADFADNLAEELDFRLEAQSMDAWVSHMHASPLGRNIRVPQVYWDLTS
                     TRVLTMERIQGVRIDDVAAIRKKGFDGTELVKALLFSLFEGGLRHGLFHGDLHAGNLY
                     VDDDGKIVFFDFGIMGRVDPRTRWLLRELVYALLVKKDHAAAGKIVVLMGAVGNIRPE
                     AEAATDLENFATPLTMQNLGDMSYAEIGKQLSTLADAYDVKLPRELVLIGKQFLYVER
                     YMKLLAPRWQMMSDPQLTGYFANFMVEVSREYEEPEESAERDGVEA"
     gene            1319..2221
                     /locus_tag="VGP27_21670"
     CDS             1319..2221
                     /locus_tag="VGP27_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853940.1"
                     /translation="MDVRTGTANSGDIDIYYEDMGDPNHPPVLLIMGLGAQLLLWRKG
                     FCEKLVDQGLRVIRYDNRDVGLSTKLTGRRTGAKLLPRMVRSFLGMRSPAVYRLEDMA
                     DDATALLDHLGIDGAHIVGGSMGGMIAQIFAARFPERTKTLGILFSSNNQAFLPPPGY
                     QQLRALLQRPADSTQEAIIANAIRVAGIIGSPGYPTPQERLRADAIEGYERAYYPAGI
                     ARHFAAVLGSGSLLHYDRQINAPTLVIHGKADRLMRPLGGRAIAHAIKGARLVLIDGM
                     AHDLPEELWDQIVGELKTNFAEAP"
     gene            2381..3256
                     /locus_tag="VGP27_21675"
     CDS             2381..3256
                     /locus_tag="VGP27_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEV7853941.1"
                     /translation="MADKGKATLKPHFEDVQAHYDLSDDFFALFLDPSRTYSCAYFER
                     DDMTLAEAQQAKVDLALGKLGLEPDMTLLDVGCGWGSTMMRAIERYDVNVVGLTLSKN
                     QAARVEKVFAESDSPRTKRVLLKGWEEFDEPVDRIVSIGAFEHFGFDRYDDFFTFAHE
                     ALPDDGVMLLHTITALTGRQMVEQGMPLTINFARFVKFMITEIFPGGRLPSIEKVSDH
                     SSKAGFTLTRRQSLQPHYARTLDIWAANLEAKKDEAIAIQSEEVYERYMRYLTGCADG
                     FRVGYIDVNQFTLEK"
     gene            3308..>4100
                     /locus_tag="VGP27_21680"
     CDS             3308..>4100
                     /locus_tag="VGP27_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEV7853942.1"
                     /translation="MVEKTAASTKKRQSVDEVRAHYDLSNEFFQLFTDPTVTYSCAYF
                     PRDDMTMEEAQIAKLDLSLGKLGLKPGMTLLDVGCGWGSMMKRAMERYDVNVVGVTLS
                     KNQHEYCQKVLAGVETNRSYKVLLQDWAEVKEPVDRIVMIEALEHFGFERYDDFFKFA
                     YDLMPDDGVMMVHSICGLTPQEMKDKGVPLTFSFARFIKFVLTEIFPGGRLPSVEKVK
                     EHSTKAGFKVTRIQSLQPDFAKTLDMWADGLESRKDEAIAIQSEEV"
BASE COUNT          768 a         1330 c         1323 g          679 t
ORIGIN      
        1 cgtcgaggcc gcccgcatcg gagcgacggg ttggcaggta acccgcaccg gcgcacgggt
       61 cgtcaccaag ctcgccggcc gcggctcact gcagcagaag gtcatccggg agattccgca
      121 gacgttcgcc gacctcggcc ccacctacgt caagttcggc cagatcatcg cgtccagccc
      181 gggcgcgttc ggcgaacagc tctcccgcga attccgcagc ctgctcgacc gggtaccgcc
      241 cgccgacagc ggcgagatcc acaagttgtt cgcggaggag ctcggcgaca agcccgagaa
      301 cctgttcaac agcttcgacg aggagccgtt cgcgtcggcg tcgatcgctc aggtgcacta
      361 tgcgaccctg cacagcggcg aggaggtcgt cgtcaagatc cagcggcccg gcatccgtcg
      421 ccgcgtcgcc gccgatctgc agatcctcaa gcgcggcgcg cagctggtcg agctggccaa
      481 gttaggccgc aggctgtccg cgcaggacgt tgtcgccgat ttcgccgaca acctcgccga
      541 agaactggac ttccgcctcg aggcgcagtc gatggatgcg tgggtgtcgc acatgcacgc
      601 ctcgccgctt ggccgcaaca tccgggtgcc gcaggtgtac tgggacctga ccagcacgcg
      661 ggtgctgacc atggagcgca tccagggcgt tcgcatcgac gacgtggccg cgatccgaaa
      721 gaagggcttc gacggcaccg agctcgtcaa ggcgctgctg ttcagcctct tcgagggtgg
      781 gctgcggcac ggcctgttcc acggcgacct gcacgcgggc aacctgtacg tcgacgacga
      841 cgggaagatc gtgttcttcg acttcggcat catgggccgc gtcgacccgc gcacccgctg
      901 gctgctgcgc gagctggtct acgcgctgct ggtgaagaag gaccatgccg cggcgggcaa
      961 gatcgtcgtg ctgatgggcg cggtcggcaa catcaggccg gaggccgagg ccgccacgga
     1021 cctggagaac ttcgcgacgc cgctgaccat gcagaatctc ggcgacatgt cgtatgccga
     1081 gatcggcaag cagctctcca cgctggccga cgcctacgac gtcaagctgc cgcgcgagct
     1141 ggtgctgatc ggcaagcagt tcctctatgt cgagcgctac atgaagctgc ttgcaccgcg
     1201 ctggcagatg atgagcgatc cgcagttgac gggctacttc gccaacttca tggtcgaggt
     1261 gagccgcgaa tacgaggagc ccgaggaatc cgccgaaaga gatggagtcg aggcttaagt
     1321 ggacgttcgc accggcaccg ccaactccgg cgatatcgat atctactacg aggacatggg
     1381 cgacccgaac catcctccgg tcctgctcat catgggcctc ggcgcgcaat tgttgttgtg
     1441 gcgcaaggga ttctgcgaga agctcgtcga ccagggtctg cgcgtcatcc ggtatgacaa
     1501 ccgcgacgtc gggctgtcga cgaagctgac cggccgccgc accggcgcaa agctgctgcc
     1561 ccgaatggtg cgctcgttcc tcggcatgcg aagtccggcg gtctacaggc tcgaggacat
     1621 ggccgacgac gccactgccc tgctcgacca tctcggcatc gacggcgcgc acatcgtcgg
     1681 cgggtccatg ggcggcatga ttgcccagat cttcgcggcc cggttccccg aacgcaccaa
     1741 gacgctcggc atcttgttct ccagcaacaa ccaggcgttc ctgccgccgc cgggatacca
     1801 acagctgcgg gcgctgttgc agcgaccggc cgactccacg caggaggcga tcatcgccaa
     1861 cgcgatccgg gtcgccggga tcatcggcag tcctggctat cccacgcccc aggagcggct
     1921 gcgggcggac gcgatcgagg gctatgagcg cgcctactac cccgcgggca tcgcacggca
     1981 cttcgccgcc gtgctcggca gcggcagcct gctgcactac gaccgccaga tcaacgcgcc
     2041 gacgctggtc atccacggca aggccgacag gctgatgcgc ccgttgggcg gccgcgccat
     2101 cgcccacgcc atcaagggtg cgcggctggt gctcatcgac ggcatggccc acgatctgcc
     2161 cgaggagctg tgggaccaga tcgtcggcga actcaagacg aacttcgccg aagcgccctg
     2221 atcagtcagg ttttgccgat tcggagctga ccccgaaatc ggctagcatt ccgatgcggg
     2281 gaggcatccg gggttgatct atggtgcgtc ccccggggcg ggtcctccac cacggctttt
     2341 cgggggatgc cttccgcaat agagaggcaa caacatcgac atggccgata agggcaaagc
     2401 aaccctgaaa ccgcacttcg aggacgtcca ggcgcactac gacctgtccg acgacttctt
     2461 cgcgctgttc ctggacccgt cgcggaccta cagctgcgca tacttcgagc gcgacgacat
     2521 gacgctggcc gaggcgcagc aggccaaggt cgacctggcg ctgggcaagc tgggtctcga
     2581 gccggacatg acgctgctcg acgtcggttg cggctggggc tccacgatga tgcgggccat
     2641 cgagcgctac gacgtcaacg ttgtcggcct gacgttgtcg aagaaccagg ccgcccgcgt
     2701 cgagaaggtc ttcgcggagt cggacagccc aaggaccaag cgtgtcctgc tgaagggctg
     2761 ggaggagttc gacgagcccg tcgaccgcat cgtgtcgatc ggcgcctttg agcacttcgg
     2821 cttcgaccgc tacgacgact tcttcacatt cgcccacgag gcgctgcccg acgacggcgt
     2881 catgctgctg cacacgatca cggcgctgac cggccggcag atggtcgagc aaggtatgcc
     2941 gctgaccatc aacttcgcgc gtttcgtcaa gttcatgatc accgagatct tccccggtgg
     3001 ccggctgccg tcgatcgaga aggtcagcga ccacagcagc aaggcgggct tcactctgac
     3061 gcgccgccag tcgctgcagc cgcactacgc ccgcacgctg gacatctggg ccgcgaacct
     3121 cgaggccaag aaggacgaag ccatcgccat ccagtccgaa gaggtctacg agcggtacat
     3181 gaggtacctg acgggctgcg cggacggctt ccgggtcggc tatatcgacg tcaaccagtt
     3241 caccctcgag aagtaagcag catcgccggc gccgtgtatg gtgcctgcga acaggacgga
     3301 gtccaagatg gtcgagaaaa cggccgcatc cacgaagaag cggcagagcg tcgacgaggt
     3361 gcgcgcgcac tatgacctct ccaacgagtt cttccagctt ttcaccgacc cgacggtcac
     3421 ctacagctgc gcgtacttcc ctcgtgacga catgacgatg gaagaggcgc agatcgccaa
     3481 gctcgatctg agccttggca agctggggct gaagccgggc atgacgctgc tcgacgtcgg
     3541 ctgcggctgg ggctcgatga tgaagagggc gatggagcgc tacgacgtca acgtggtggg
     3601 cgtgacgctg agcaagaacc agcacgagta ctgccagaag gtgctcgccg gcgttgagac
     3661 gaaccgctcc tacaaggttt tgctacagga ctgggcggag gtcaaggagc ccgtcgaccg
     3721 gatcgtcatg atcgaggcgc tggagcactt cggattcgag cgctacgacg acttcttcaa
     3781 gtttgcctac gacctgatgc ccgacgacgg cgtgatgatg gtgcattcga tctgtgggct
     3841 gaccccccag gagatgaagg acaagggcgt cccgctcact ttctctttcg ctcgattcat
     3901 caagttcgtc ctgaccgaga ttttccccgg cggccggttg ccgtcggtcg aaaaagtcaa
     3961 ggagcattcc acgaaggccg gcttcaaggt gacccgtatc caatcgctgc agccggactt
     4021 cgccaagacc ctcgacatgt gggccgacgg gctggagtct cgcaaggacg aggcgatcgc
     4081 gatacagtcc gaggaggtct
//
LOCUS       DASYLX010000930         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3895600,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000930 DASYLX010000000
VERSION     DASYLX010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3895600"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGP27_21685"
     CDS             complement(<1..881)
                     /locus_tag="VGP27_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV7853943.1"
                     /translation="MSAPHIALHPTALTETGIRGLVRVLGGPGTGKTRALIDAAAAHI
                     ADGAEPESVLLLTGSARLGAQARAAITAALLQAGSRAVVREPLVRTVHSYAFAVLRLA
                     AQRNGDPPPRLITTAEQDGIIRELLAGDLEDGDNAAVAWPRQLRPALSTVGFATELRD
                     LLARCTERGVDPSALQRIGRSSGSPEWLAAGQFAQQYEQTMLLRSAVGMAAPQATVPA
                     LGAAELVGAALEAFAVDAELLAAERARVTLLLVDDAQHLDPQAARLVRVLAAGAELTI
                     VAGDPNQSVFSYRGADPA"
     gene            888..1742
                     /locus_tag="VGP27_21690"
     CDS             888..1742
                     /locus_tag="VGP27_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7853944.1"
                     /translation="MTPRGSDKACRPDALSIGAVVSNPHFLNVHRFGPEQPAQILAIH
                     GLTGHGLRWRTLATQHLPGFSVLAPDLLGHGRSSWEAPWTLDSNVTALADLLASETAR
                     PVVVVGHSFGGAVALQLAVARPDLVASLVLLDPAVGLDGAWMANIADDMMASPYYLDR
                     DEARNEKATGSWADVDPIELDAELDEHMIERPDGRCGWRISIPAMMSYWSELARDFVV
                     PHKGIRTTLVRAARTRPPYVTDALITALDTKLGPRFSLVDFDCDHMVAQAKPAETAAV
                     IRDRLEHA"
     gene            1745..2041
                     /locus_tag="VGP27_21695"
     CDS             1745..2041
                     /locus_tag="VGP27_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEV7853945.1"
                     /translation="MAAITDEQVEAVRALVASIPSGRVSTYGDIASAAGLSSPRIVGW
                     IMRTDSSDLPWHRVVRASGRPAPHLRTRQLERLRAEGVLASDGRIDLAEARHEF"
     gene            complement(2044..>2557)
                     /locus_tag="VGP27_21700"
     CDS             complement(2044..>2557)
                     /locus_tag="VGP27_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02569 family protein"
                     /protein_id="HEV7853946.1"
                     /translation="PRFLTQPPVAPWADVDVFIAADRAAWEERPLQSLQAGARVSPGS
                     ADGQRSIDLINQLAGLRKPTKSPNQLVHGDLYGTVLFAGTAAPGITDITPYWRPSSWA
                     AGVVVVDALSWGEADDGLVERWTPLPEWPQMLLRALMFRLAVHALHPRSTAAAFPGLA
                     RTAALVRSVL"
BASE COUNT          396 a          908 c          900 g          353 t
ORIGIN      
        1 gccggatcgg cgccacggta ggagaacacg gattggttgg ggtcaccggc gacgatggtc
       61 agctcggcgc cggccgcaag cacccgcacc aggcgggcgg cctgagggtc gaggtgctgg
      121 gcgtcgtcga ccagaagcag cgtgacccgc gcgcgttcgg cggcgagcag ttcggcatcg
      181 acggcgaagg cctccagcgc ggcgcccacg agttcggctg cgccgagtgc gggcacggtg
      241 gcctgcggag ccgccatgcc gacggccgac cgcagcagca tcgtctgctc gtactgctgc
      301 gcgaactgac ctgcggcgag ccactccgga ctcccggacg accggccgat gcgttgcagc
      361 gcagaaggat ccacaccgcg ctcggtacag cgggccaaca ggtcgcgcaa ctcggtggcg
      421 aacccgacgg tgctcagcgc ggggcgcagt tgacgcggcc acgcgaccgc cgcgttgtcg
      481 ccatcctcca gatcgcctgc gagcagttcg cggatgatgc cgtcctgctc ggcggtggtg
      541 atcaggcgcg ggggcggatc accgttgcgc tgggcggcca gcctcagcac ggcgaaggcg
      601 tacgagtgca ccgtgcggac cagaggctcc ctgacgacgg cgcgactgcc cgcttgcagc
      661 agcgccgctg tgatggcggc gcgcgcctgc gcgcccagcc gcgccgaacc cgtcagcagc
      721 agaacagatt ccggctcggc accgtcggcg atgtgggcgg ccgcggcgtc gatcagcgcg
      781 cgggtctttc ccgtacccgg gccaccgaga acgcgcacca gaccgcgaat gccggtttcg
      841 gtcaacgccg tcgggtgaag cgcgatgtgc ggtgcggaca tgagggcatg acaccacgag
      901 ggtccgacaa ggcgtgccga ccggatgccc ttagcatcgg agccgtggtc agcaaccccc
      961 atttcctcaa tgtccatcgt ttcggtcccg aacagccggc gcagatcctg gcgatccacg
     1021 ggctgaccgg acacgggctg cggtggcgca cgctggcgac gcagcacctg cccggcttct
     1081 cggtgctcgc gccggacctg ctgggccacg gcaggtcgtc gtgggaagcg ccgtggacgt
     1141 tggactccaa tgtcaccgca ctggccgacc tgctcgcctc cgagacggct cgccccgtcg
     1201 tggtggtcgg gcactccttc ggcggtgcgg tcgcgctgca actcgccgtc gcgcgcccgg
     1261 acctcgtcgc ctcgctggtg ctgctcgacc cggcggtcgg cctcgacggc gcgtggatgg
     1321 ccaacatcgc cgacgacatg atggcctcgc cgtactacct cgaccgcgac gaggcgcgca
     1381 acgagaaggc gacgggttcg tgggccgacg tggatccgat cgaattggac gccgaactag
     1441 acgagcacat gatcgagcgg cccgacggcc gctgcggctg gcgcatcagc attccggcga
     1501 tgatgtccta ctggagcgag ctggcacgcg atttcgtcgt gccgcacaag gggattcgca
     1561 ccacactggt ccgcgccgcc cgtacccggc caccgtatgt caccgacgca ctgatcaccg
     1621 cgctggacac caagctcggc ccccggttct cgctcgtcga tttcgactgc gatcacatgg
     1681 tggcccaggc caagcccgcg gagacggccg cggtgatccg cgaccggctc gaacacgcct
     1741 gaccgtggcc gccatcaccg acgaacaggt cgaagcggtg cgcgcgctcg tcgcgtcgat
     1801 cccatcgggc cgggtctcga cctacggtga tatcgcctca gccgcagggc tttccagccc
     1861 gcgaatcgtc ggctggatca tgcggacgga ttcgtcggat ctgccgtggc accgggtggt
     1921 ccgggcctcc ggacggcccg cgccgcatct gcgcacgcgc caactcgagc gactgcgggc
     1981 agagggtgtg ctagccagtg acggccggat cgacctggcc gaagcgcgcc acgagttctg
     2041 acctcagagc accgaccgca ccagcgctgc ggtgcgggcc agtcccggga acgccgcagc
     2101 cgtcgagcgc gggtgcaggg cgtgcacggc cagccggaac atcaacgcgc gcaacagcat
     2161 ctgcggccac tccggcagcg gagtccaccg ctcgaccaga ccgtcgtcgg cctcgcccca
     2221 cgacagcgcg tcgacgacga ccacgccggc cgcccatgaa ctcgggcgcc agtagggcgt
     2281 gatatcggtg atgccgggcg ccgcggtgcc cgcgaaaagc actgtgccgt acaggtcgcc
     2341 gtgcaccagc tggttggggc tcttggtggg cttccgcagt ccggcgagct ggttgatcag
     2401 atcgatcgag cgctgaccgt cggccgatcc gggcgacacc cgcgcgcccg cctgcagcga
     2461 ctgcagcggc cgctcctccc acgcagcccg gtcagcggcg atgaagacgt cgacgtcggc
     2521 ccacggcgcg accggcggct gggtcagaaa ccgggga
//
LOCUS       DASYLX010000931         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1482825,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000931 DASYLX010000000
VERSION     DASYLX010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482825"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VGP27_21705"
     CDS             complement(<1..635)
                     /locus_tag="VGP27_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEV7853947.1"
                     /translation="MRDSRGFDFTGYKRASLIRRVQHRMDKAGQNSYDEYLDTLQAST
                     DEFAALFNTILINVTTFFRDPEAWDYVRTDIIPGLLASRGPTDPIRLWSAGCASGQEP
                     YSLAMLFAEELGLETYRQRVKIYATDVDEEALTQARAAVYDAKAVESVPAELRERYFD
                     RNNGNYIVNKDLRRAVIFGRNDLVKDAPISRVDLLSCRNTLMYMNGDTQRNV"
     gene            complement(743..1504)
                     /locus_tag="VGP27_21710"
     CDS             complement(743..1504)
                     /locus_tag="VGP27_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7853948.1"
                     /translation="MPNSQAPNLVVGSDTVGRTTVLTIEGVLDATTYLLLRDCIIKAA
                     LDDPWAVIVDMGELFVPRESALAVFTSASWHVERWPEVPILLVCGHHAGRDSLARNGV
                     TRYVPVYPSVESACDAADLIRKKPLRRRVRIELPSEVTSLGRSRRFVADTLSAWSMPD
                     WIPVTKIVVTTFVENVLAHTESQAGIRLETDGNTVTVAVADSNHSSANIREPAQATGV
                     PTGLGIVGSVCRMWGNAPVPSGKIVWAVIGSENIL"
     gene            complement(1494..2516)
                     /locus_tag="VGP27_21715"
     CDS             complement(1494..2516)
                     /locus_tag="VGP27_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV7853949.1"
                     /translation="MTTTNGHPNAPLRGVVAIGASAGGVEALIDLASHLPDDLPYAVL
                     VTLHLPPNAPSVLADIIDRSGPLPAVAASDGDKLEAGKIYVAVPDHHLLVDDHHVVLS
                     EGPTENGHRPAINALFRSVALSIGPNAIGVVLSGVLDDGVAGAAAIRARGGTTVAQRP
                     EDALFSAMPQNALGAGVIDHEAAATEIGPLLVRLADREIEERAMEPDASLELENRIAM
                     AKKFSTQFDGELLGPHSGYICPDCNGSLMVVNGNNFRCRVGHAWTGEALLDARDDEVE
                     QALWIALRSLQEKARLARRMADNVGSGVLHKRYTETAEEAEHAMSVLGKRLSGVSPMA
                     DEAGAQ"
     gene            complement(2583..>2886)
                     /locus_tag="VGP27_21720"
     CDS             complement(2583..>2886)
                     /locus_tag="VGP27_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7853950.1"
                     /translation="RRAVRCDGWMHGGSGRKELDRLLNRLNEIRNEEGCTKPFEIHVM
                     SADAFTTDGIKRLEDKGVTDAIVGFRNPYVMGNDDEPLDTKVRHLESFAEKVMAKV"
BASE COUNT          492 a          914 c          921 g          559 t
ORIGIN      
        1 acgtttcgct gcgtatctcc attcatgtac atgagcgtgt tgcggcagga gagcagatcg
       61 acccgggaga tcggcgcatc cttcaccagg tcgttgcggc cgaagatgac ggcgcgacga
      121 aggtccttgt tgacgatgta attgccgttg ttgcgatcga agtagcgctc gcgcagctcg
      181 gcgggcacgg attcgaccgc cttggcatcg tagacagcgg ctcgagcctg ggtcagcgcc
      241 tcctcgtcca catcggtcgc gtagatcttg actcgctgcc ggtaggtttc cagaccgagc
      301 tcctcggcga acagcatggc caggctgtag ggttcctgac ccgatgcaca gcctgcgctc
      361 cacagcctga tcggatcggt cggaccgcgg ctggccaaca ggcccggaat gatgtccgtg
      421 cggacgtaat cccacgcctc ggggtcacgg aagaacgtgg tgacgttgat cagaatggtg
      481 ttgaacagcg ctgcgaactc gtcggtgctg gcctgcagcg tatcgagata ctcgtcgtag
      541 gagttttggc ccgccttgtc catccggtgc tgcacccgcc ggatcagcga agcgcgtttg
      601 tatccggtga aatcgaatcc gcgcgagtcc cgcatatagc gcaagaggct ttcgaacgag
      661 tcgtcgagct gcccgtcgcc gtcgccgtcc aatgttcgcc cctatccgcc cactgttacc
      721 gcgaattcga acatactcgg gcctagagga tgttttccga gccgatgacc gcccagacga
      781 tttttcctga cggcacgggc gcattgcccc acatccgaca cactgatccc acgatgccca
      841 gaccagtggg cacgccggtg gcctgcgcgg gctcccggat gttggctgac gagtgattgg
      901 agtccgccac tgccaccgtc acggtgttgc catcggtttc gaggcggata cccgcttggc
      961 tttcggtgtg cgccagcacg ttctcgacga aggtcgtcac gacaatcttc gtcaccggga
     1021 tccagtccgg catcgaccat gcgctcagtg tgtccgctac gaaccggcgg gaacgtccga
     1081 ggctggtgac ttcggaaggt agctcgatgc gcacgcgtcg acgcaggggc ttcttcctga
     1141 tcagatccgc tgcgtcgcaa gcagattcga cactcggata cacgggcacg tagcgggtca
     1201 cgccgttacg ggcgagggaa tcccggccgg catgatgacc acacaccagc aggatcggca
     1261 cctccggcca gcgctcgacg tgccaactgg cgctggtgaa gacggcaagg gccgactctc
     1321 gggggacgaa cagttcgccc atgtcgacga tgacggccca tgggtcatcc agcgcggcct
     1381 tgatgatgca gtcgcgcagc agcaggtagg tggttgcgtc gagcactcct tcgatggtga
     1441 gtacggtcgt ccggccgacc gtgtcggacc cgacgacgag gttgggcgct tggctattgg
     1501 gcaccggcct catccgccat aggggagact cccgacaggc gtttgcccag aaccgacatc
     1561 gcgtgctcgg cttcttcggc tgtctcggtg taccgcttgt gcagaacgcc cgaaccgacg
     1621 ttgtcggcca tcctgcgcgc cagccttgcc ttctcctgca ggctgcgcag ggcgatccac
     1681 agcgcctgct cgacctcgtc gtcacgcgcg tccaggagtg cctcgcctgt ccaggcgtgc
     1741 ccgacccggc aacggaagtt gttcccgttg accaccatca gggagccgtt gcagtccggg
     1801 cagatatatc ccgaatgcgg tcccaataat tcaccgtcga actgggtcga gaactttttt
     1861 gccatcgcaa tccggttttc caattccagg ctggcgtcgg gttccatggc ccgctcctca
     1921 atctctcgat cggccagtcg cacaagtaat gggccgatct cggttgcggc ggcttcatgg
     1981 tcgatcacgc ctgcgcctaa ggcgttctgc ggcatcgccg agaacaaggc gtcctcggga
     2041 cgttgggcga cggtggtgcc gcctctggct cggatcgccg ccgcgcccgc gacgccgtcg
     2101 tccagcactc ccgaaagcac cacgccgatc gcattcgggc cgatgctgag agcgaccgat
     2161 cggaagaggg cgttgatcgc gggccggtga ccgttctccg tgggcccctc cgacagcacc
     2221 acgtgatgat cgtcgacgag aaggtggtgg tcgggcaccg ccacatagat cttcccggcc
     2281 tccagcttgt cgccgtccga ggccgcaacc gcaggtagcg gtccgctccg atcgatgatg
     2341 tcggcaagca cgctcggcgc gttcgggggc aggtgcaagg tcaccagaac cgcgtagggc
     2401 aggtcatccg gaagatggga ggcaaggtcg atgagagcct cgactccgcc tgcggacgcg
     2461 ccgatggcca cgacgccgcg gagcggggcg ttgggatgac catttgtagt cgtcatcatc
     2521 aagggggtac ccctgcctta agcaggtaat cgtacctccc cggtggcgcg acggttcgcg
     2581 gtctaaacct tggccatcac cttttcggcg aacgattcga gatggcggac cttggtgtcg
     2641 agcggctcgt cgtcgttgcc catgacgtag gggttgcgga agccgacgat ggcatcggtg
     2701 acgcccttgt cctcgagccg cttgatgccg tcggtcgtga aggcatcggc ggacatgacg
     2761 tgtatttcga acggcttcgt gcaaccttcc tcgttgcgga tctcgttgag ccgattgagg
     2821 agcctgtcga gctctttgcg accactgccg ccgtgcatcc agccgtcgca gcggaccgcg
     2881 cgacgc
//
LOCUS       DASYLX010000932        24322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_385645,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000932 DASYLX010000000
VERSION     DASYLX010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24322
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385645"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGP27_21725"
     CDS             complement(<1..843)
                     /locus_tag="VGP27_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853951.1"
                     /translation="MRRLVLEADHEAFRETVRQFIEQELVPNAEKWEADRLVDRSAYI
                     AAGKHGLIGFNMPEEYGGGGVEDFRFNAVIDEEIARYGGPAPSLSLQNDVVGPYFSSL
                     SNDEQKQRWLPGITSGELIVAVAMTEPGAGSDLAGIRTTAVRDGDDWIINGAKTFISS
                     GINCDLVVVVCRTDPDAGHKGFTLLVVEEGMEGFTRGRKLEKMGLHYQDTAELNFDNV
                     RVPSANLLGKEGRGFYHLMHNLPSERLSIAISAIAGARETWRQTLQYAKDRKAFGQPI
                     GSFQH"
     gene            complement(853..2148)
                     /locus_tag="VGP27_21730"
     CDS             complement(853..2148)
                     /locus_tag="VGP27_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7853952.1"
                     /translation="MTTAEKSDADTAAVEGRITDEDIERAKAQIGIAVNQKDEAWNKL
                     PSADAITHFAFGCGDDNPLFHDPQYGNTTRWHGQIASPTFPISTGLDQTPKFTDPERK
                     KLFRGLFRGTGKYYSGVKWTWYQPVYVGRPVLMETYTLAVDVKESRFSGGRSVKETYR
                     YLYVDADGNPIATRDESYINAERQGSKKSGKLAGIERQHWTEEDFAEVEAAYEAEERR
                     GAEPRWWEDVNVGDELPMVMKGPLTVVDIISMHMGWGWGGYGVGPLKFAHQLRKRMPA
                     FYQPDEYGVPDVVQRLHWDPNRAQALGIPAPYDYGQMRAAWVSHLLTNWIGDDGWLAE
                     MDLQMRGFNYHGDTHRCTGTVTAKGQGPGETVSIDVFATSQRDEATTKGSAKVLLPST
                     ETGAVVLPVPDVDLRRRGAQVVSRMSGKVGEEMRRLHGE"
     gene            complement(2159..3007)
                     /locus_tag="VGP27_21735"
     CDS             complement(2159..3007)
                     /locus_tag="VGP27_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7853953.1"
                     /translation="MGRVQDKVVLVTGGARGQGRSHAVKLAEEGADIILFDICHDIEN
                     NHYPLATPRDLEEAGLEVEKTGRKAYTAEVDVRDRAAVTREIANAVAEFGRLDVVVAN
                     AGICPLGSDVPIGGFVDAFDVDFVGVVNTVHAALQYLQAGGSVITIGSVAGLIAETAA
                     GSVGPMGPGGEGYNLAKQFIDRYTKALAKQLAPLSIRANVIHPTNVNSPMLNSEPMYR
                     TFRPDLENPTRDDALLAWPVMQAMPIPYVEPEDITNGVCFLASDESRYVTGLQFTIDA
                     GATLKF"
     gene            complement(3019..4527)
                     /locus_tag="VGP27_21740"
     CDS             complement(3019..4527)
                     /locus_tag="VGP27_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7853954.1"
                     /translation="MTSIQTALGRLWNAPDDARMIQRDGQWLTWGHVRELTERIDREL
                     SSAGCGPGGRVAVVLGNRAESVAAIIAIFRAGRTLVTISPLQPAERLSADLVASGADY
                     VLGPQDLWSEPLFADAVEELGASAFSVDGTEVAVRAKAAHEPRQGNPAIAIEMLTSGT
                     TGPAKRIPLGRRQLEVSLSAALQHNNRPQSSDKPPLTGSVGMVTLPIVHIGGLWSLLQ
                     SLVAGRPIVMLERFSVSGWHNAIKEHRPVLVGLPPPAIRAVLNSDIPREDLASVRAIT
                     SGTAPVDPAMVDAFLQKYGIPILITYGATEFAGAVAGWTAKDFRASWEAKKGSVGHPF
                     PGVRLQVVDEDGAELPVGQTGRLQVASAQVGDSADRWVTTSDLAHIDSDGYLFIDGRA
                     DDVIVRGGFKIAPETVVRALRTHDAVADAAVAGLADDRLGQIPVAAVELRSAMTATGD
                     ELRAHCRASLTPYEVPAKVFVVDELPRGAALKVDRRQLVATLEKLTAVAQPD"
     gene            4621..5610
                     /locus_tag="VGP27_21745"
     CDS             4621..5610
                     /locus_tag="VGP27_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7853955.1"
                     /translation="MDFGLTGEQEQLADAERSWLGRNDPIERLRQALDKAPITVDPAA
                     VAHAADSGLLALLTPDVGGTHVDLAVCSEEHGYAASSLPLADIAIACWLLERAGLPEA
                     ETAAGGELLVGLARAVDLVTSPVPMAAEMTGFAVVRRTDDGEFVALVREPKLSAMTTL
                     DLSRSWARLDLDGSALDWVALPAGTLRHVRDALAVHRAFDALGGASRLLEMTVSYALQ
                     REQFGAAIGSFQAVKHHCANMAVGVEAGRAALWAGALSLDEEPDDARSRACSAAVAYA
                     KSAASEVAGTALQVHGGIGFTWEHDLHLFLRRIKVDEAVDGTVAEHRAALVAV"
     gene            complement(5617..6801)
                     /locus_tag="VGP27_21750"
     CDS             complement(5617..6801)
                     /locus_tag="VGP27_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853956.1"
                     /translation="MEYGMGPELAEFRAEVRAFIAEHAPAIPPRAGVRSAENDAEFKA
                     LQEWTGRLYEAGYAGSDWPPEFGGRKDWSPEHAIVVGEELARAQAPGAQSGSVLASHA
                     LIHYGTDEQRRRHLPEIRAGRQLWCQLFSEPGAGSDLASLRTRAVRDGDTFTIDGQKV
                     WTTDGHWARYGYLLARTDSDAPKHKGMSAFILDMSAPGVSVRPLRELTGTSDFNEVFF
                     DGVEVPADAMIGAPGQGWAIANATLAHERTNVGGIVVKLRLAVDALVDLARRITVGGR
                     PAIDSDRVRDRIGEFTAEVEALSALTYANLTRWLRGGERMHDAAMGKLMFSELNLEMA
                     QFAVELSGEAGVLVEGDDNVLDDGRWQDEWLYARAYTIAGGSSEIMRNLIAERGLGLP
                     RK"
     gene            complement(6814..9075)
                     /locus_tag="VGP27_21755"
     CDS             complement(6814..9075)
                     /locus_tag="VGP27_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7853957.1"
                     /translation="MPSADWRSRLEGLLAGYRRDQRAETGKPNRIESARAWHARLVDN
                     ELAAPGWPKSVGGLELSLEDQLDYYRLTTAAGAPPHPCPLSFILAPTLIEHGTAEQKA
                     RFLTPLLRADEFWCQGFSEPGAGSDLASLSTRAVRDGNVYRVTGQKVWTTMADRADWM
                     FALVRTGPPGRSTEGITYLLIPMDSSGITVRPLRDISGAAHFAEVFLDDVEVAVENRV
                     GDEGRGWSIMRTSLGHERATAFLADEFKYRKTADRVIELVASGQLDADPQVRQDIARL
                     ESGVRTIAANSARALAAVLRAEDPGGVASVNRLVKSEFEQHMHALALRAVGPHAALGS
                     RAAAAIDKGRWTFGYLMSRATTIGAGTAEIQRNTIAESVLGLPSHRGEGSRAAAVVPG
                     APLAVADEDERELRDVLSQTLRARVDVETLLDPKRAHDACDSAAWSALVEFGLPGLAV
                     DEALGGEGARPQLLYAAIEEAGKALAPVPLVPTVTALGVAVAVGAKEAAQRIAAGAPA
                     AFAVPLDDSGWLTAGPQLPEWDGERLSGSVPIVAGAPSAEVLLVLARTTGDGEALVAV
                     DPAGDGVEVTPQQPLDLTATVGALTFTAAAGEVLSDGADLRNGLRDARRQALLAVAAD
                     SVGVASLALAMSVEWAGERQQFGRPIGSFQAISHRCADMLVALEGARSQVLAAAQADA
                     DEAEYLVDLAAAAALDAAVSATEGALQIHGGLGFTWEHPVHLLLRRAAANAVLVGRAD
                     ALRDRAAGAVLSR"
     gene            complement(9153..9347)
                     /locus_tag="VGP27_21760"
     CDS             complement(9153..9347)
                     /locus_tag="VGP27_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7853958.1"
                     /translation="MKVRLEQAKCVGHAQCYAVDPDLFPIDDSGYSILEAHDVKPGDE
                     QQTRDGVAACPELALILEED"
     gene            complement(9364..9843)
                     /locus_tag="VGP27_21765"
     CDS             complement(9364..9843)
                     /locus_tag="VGP27_21765"
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEV7853959.1"
                     /translation="MCRRISGRGTEVIVNNRFIAGASALVVACGVAGCSDPAPAARPA
                     GGLPAGTAEVSINGAGAGRSNAVNCSTVGPVTTINTGNESSGTTSTIDTRDGLAVQFA
                     QITDVGGFTGSYWAHLGPDAKVDMTGQTFVVTGSATGFNVENPSARATEDFSIRVAC"
     gene            complement(9925..11103)
                     /locus_tag="VGP27_21770"
     CDS             complement(9925..11103)
                     /locus_tag="VGP27_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005184679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7853960.1"
                     /translation="MPLRDHHQIVSVDDHLVEHPRVWQDRLPAKWREHGPRIVEMDGK
                     HLWSYDGQIFPTIGLNAVAGKPPEEWGMDPVRYEDMIPGCYDPVARVADMDLDGVQTA
                     LCFPSFPGFGGGTFHRAADKDLALLCVKAWNDFYIEEWCATAPDRYVPLAILPTWDVK
                     ACVDEAERVAAMGARTVSFPDSPVPLGLPSFHSDHWDPLWRVCSDAHMPLSLHFGSGS
                     YVPGFSFSSMAPVAGQMQMPDAPFAVAITLFSTNLMWSTVDWLFSGKLQQFPDLQISL
                     AEGGIGWVPYILERADYVWERHRYYQPIDFDARPSDLFRKHFWGCFIDDEHGLENRHS
                     VGIDRIMLEIDFPHSDSNWPNSRKRAAEVLAGVPDDECSLIVEENARRMLNFPRSTVA
                     "
     gene            11204..11518
                     /locus_tag="VGP27_21775"
     CDS             11204..11518
                     /locus_tag="VGP27_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7853961.1"
                     /translation="MSATEHTPDAEARGKLDEDDHDLLTFGEAGERLRLEITAAHDAV
                     TRLSECGDTAGLEKARHRLAALRAAADRNSAQPINDANFEKFFGYPGKAKRNLPQAPA
                     AE"
     gene            11515..12801
                     /locus_tag="VGP27_21780"
     CDS             11515..12801
                     /locus_tag="VGP27_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7853962.1"
                     /translation="MTTAADKLRRPGYAPPANAVVGRRGLHTRDRIVSCAAEVFLQNG
                     FHSTSIDTIAKSAAASRATVYQYFADKEAIFAELSRAAVRSVLGLGERLGPLGPTADG
                     LANLEAWMHEWADIYDAHAAVFAEYPGIGTVMGLTAVDAHSVPERFTRHVTDRLRSAP
                     VRGLEIADAVAALMRIPHMVNLYRHRGLFGLPSRQTVSGSLAIALQLMLFPDTADTMT
                     FAAVAPGLDVAVPVAHDGRKPSPIHQDVLSVSSQLFAQRGYYAVGMADIAAAADISRA
                     TLYRYFSTKDMILAELTRAAVLEAEHHAAALKLLTINGVDAEQFSRWTHDYVRFHRAY
                     SGVIRAWFDGTVAERLSEAAAGDGIASIRDAVAALLQTATLPAGVDPATAQAVFLAIL
                     GRMTEPTASGQSDAHAAEVIVALLGRSLLSQLGAAT"
     gene            complement(12780..13139)
                     /locus_tag="VGP27_21785"
     CDS             complement(12780..13139)
                     /locus_tag="VGP27_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7853963.1"
                     /translation="MKTYPLISLASAALFCTGGVIAGTPTASADAVAYLVNVTVKPGY
                     NFANADAALAYGHGVCDKIAQGRPYAQLVGDIKGDFNTNDEFQASYLISQAANELCPA
                     QIWQLRNSAAGYVAAPS"
     gene            13206..13697
                     /locus_tag="VGP27_21790"
     CDS             13206..13697
                     /locus_tag="VGP27_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV7853964.1"
                     /translation="MTRSKAGRFGLTIPLLVVVASATVIPTAHADNKRLNESVFVNID
                     TAQKQNGCPGEAHLDGRLVEAARLHTLDVLNNRALDGDIGSDGSTPQDRANAQGFVGK
                     ASETVAINPALAISGIEILGQWWYNPAYKAIMQDCSNLAIGVWSENSLDRTVVVAVYG
                     QPA"
     gene            complement(13694..15235)
                     /locus_tag="VGP27_21795"
     CDS             complement(13694..15235)
                     /locus_tag="VGP27_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7853965.1"
                     /translation="MTLGDVVTDNAVRFPDVPAYRSGSRMVTHAQLRDRAVRLVSALA
                     AVGVRRQDRVAVLSRNSIEFGEVMAASQLSGIIMATVNFRLSALEVLDVLDRVAPSVV
                     FCEDEFAAVITELAPRLPSMPLLVCIGGTARSDMVAFEEFITSGDCGQLAFAARPHDI
                     ACLLFTSGTTGASKCCILGQRELRRVAFTMNVEMRSGSADRGLINMPMFHVGAMAIIG
                     GLHARGGTVVLQQQFDTAEAVRIIAEHNITVLHLAPVMLRALLDVVTDARDLETVRTV
                     IYSAAPITADVLARAMSALPSAGFLNLYGQTEAIVSGLPRELHVLDGPDAGEALRSVG
                     FPFPGVRVRIVGDDGCDVPVGEAGEIVVRSDSVFRGYWDDPAATLATLRDRWCRTGDV
                     GRCDGRGLLYLVDRKKDVIITGGENVFSPEVEDVLSAHDAVAACAVIGAPDEKWGEAV
                     CAVVVPTAGATLTLEAVQDFVRSRLAGYKVPRRLVFVAELPVLASGKVDKKRLRADVA
                     GAAAG"
     gene            complement(15262..16455)
                     /locus_tag="VGP27_21800"
     CDS             complement(15262..16455)
                     /locus_tag="VGP27_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7853966.1"
                     /translation="MTRGPFDGKAVLTGAGKSQVGRRLGRTGLDLTVEAVLRAIEHAG
                     LAVDDVDGIASYPGPGVPDLGFSGASVQDVRNVLGLRSRWYMSGLETAGQIGPAIEAC
                     MAVTLGLANHVVVFRSVWESTAAEQAGGGRASVLFTGGKLPPQLEWVAPFGALSATNW
                     LAMPAQRYMHDFGLTREQLGWIALNARRNAGLNPDAVYRQPMTMDDYLSARMISEPLC
                     LYDCDAPCDGATAVIISRRDAASGLPRHPLTVESVGPGMFERATWDQRSDLTTMAAHD
                     SAATLWQNTALTPADVDMAQIYDGFSFLTVMWLEALGFCEHGKVGEFIEGGERIALDG
                     SLPINTSGGQLSGGRLHGMGFLHEACVQMWGEGGDRQVPQTPEVVAVGVGGGPVAGSM
                     LLSSR"
     gene            complement(16473..16931)
                     /locus_tag="VGP27_21805"
     CDS             complement(16473..16931)
                     /locus_tag="VGP27_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7853967.1"
                     /translation="MHTIGSIDSAIALIGAELGVSRWVDIDQSRIDAFAEVTMDHQWI
                     HVDVEKAKAESPYGATIAHGFLTLSLIPGVSKDNYRVENAKMGINYGLNKVRFLAPVT
                     AGSRVRVRSELADATKVADDTVNLTVRHTVEIDGVDKPAAVAELIARFIF"
     gene            17040..18227
                     /locus_tag="VGP27_21810"
     CDS             17040..18227
                     /locus_tag="VGP27_21810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7853968.1"
                     /translation="MATTTSRAAIVAAARTPIGTARRGTLANVPAIELAKPVVTAVVE
                     RSGLDAADFDDFVLAESMQGGGDSARYIAVDLGLTDIPGIAVNRQCASSLSAIAVGAG
                     QIAAGMSRAILAGGMESLSTGPIMQKRKPFTSGKSPEDYAQWFPESHPPTAEAPAMDM
                     SITVAHNCNVQYGITREDQDEWALRSHQRAIMAIDAGSFSDEIIPIQVPQADGTAITF
                     AEDEHPRRGSSMESLAGLKVLHPEIEGFTVTAGNSSGINDAAAVVALASPDTQQNALA
                     NVLSWTQVGLDPTRTGSGPIKAIPKALELAGRKLEDVALFEINEAFAAQAVACARELG
                     LDEEIVNVYGSGISLGHPIAATGARMVTSAIYELRRRGGGIGVLSMCAGGGMGAAMVI
                     EVV"
     gene            18229..18654
                     /locus_tag="VGP27_21815"
     CDS             18229..18654
                     /locus_tag="VGP27_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7853969.1"
                     /translation="MTALITEGLFRVDGERAVLFGSRRRSTGVVKFPAERPELFDGSP
                     DVQADIEPLELSTEGTLYTFTTQEFAPPLPYKGNRDPKVFKPYVVGFVELPEGLLVES
                     LIIDAAASDLRIGQRLVSATTTLDTADGESLLTFAFRAA"
     gene            18665..19684
                     /locus_tag="VGP27_21820"
     CDS             18665..19684
                     /locus_tag="VGP27_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEV7853970.1"
                     /translation="MTDLGTPEFALHPLHGIHQPTTGNPARRPGSARRTSSIDMTRDE
                     GSLDPVYLQGRARDLWTAADGSVSEVGAAELSATIELVARVVRHVEVVPPVAGVAGLS
                     GAPAMSGFRAAADKAAPELRQHRDLRYTLLDDVPVATLISGHSLSASNVLAHFTKSGY
                     LPVADQCAGFATGGLLMTSFEAGDPVIVTGPEAPDLDHDSDRLAWHPMAQLPRHGMRR
                     RRRIDVFDAGEDSRVGIDAMFRDTYVRADGAETIIHEYTLAAAVDADTGVIVESHATP
                     RVLPWQECPGAVASAERITGMTLGELHFRVRQELFGTSTCTHLNDLLRSVADAEVLIG
                     LCRGT"
     gene            19825..20772
                     /locus_tag="VGP27_21825"
     CDS             19825..20772
                     /locus_tag="VGP27_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853971.1"
                     /translation="MARTDDDTWDINESVGATALGVAGGRAAETDSADPLISDPFAKL
                     FLDAAGDGIWRIYLDAELPAELVDLDPELPVRMQAMMGYTACRTKFFDEYFVAAAGDG
                     IRQAVILAAGLDARAWRLAWPAGCVVYEIDQPKVLAFKTSTLESHQATPIASHVSVGI
                     DLRLDWPKALAKSGFDPSVPTAWLAEGLLPYLTADAQDVLFDRIQSLSAPGSRVAVEA
                     FTNDFFSQESFARREEQMDRYRAAAIKLGGRDITESGNLLYEEERTEVVVWLRAHDWA
                     VTTATSATDLMAANNRAVPPGLDDAVPESVFVEAISGAS"
     gene            complement(20753..21529)
                     /locus_tag="VGP27_21830"
     CDS             complement(20753..21529)
                     /locus_tag="VGP27_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014928328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7853972.1"
                     /translation="MDINGASAIVTGGASGIGAASARQLAARGARVVVADVQADRGTA
                     LADDIGGVFVAVDVTDTEQIAAAVNRATQLGPLRVLVNSAGIGGAQRTIGKDGQFASA
                     HNLDAYKKVIAVNLIGTFDCIRLAATAMSRNEPLDSGERGAIVSMASVAAFDGQIGQA
                     AYSSSKGGVVGMTLPVARDLSAVGIRVNTIAPGLIDTPIYGEGEKSEAFKAKLGESVL
                     FPHRLGNPEELASMVVELVTNSYMNAEVIRVDGGIRMPPK"
     gene            complement(21529..22800)
                     /locus_tag="VGP27_21835"
     CDS             complement(21529..22800)
                     /locus_tag="VGP27_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7853973.1"
                     /translation="MSADRCGLDFEPFKPGPHEQLPTVYAQLRERFPVYRTRSNIWVI
                     SRFDDVKTVQSTPDVFSSRPNPYEGESAASDAEMKPEVIERLMALAAGIPVDMNEMAS
                     AKTIAAADPPQHTRIRRIVSRGFTPPRIKEMGNAISSIVERCLAGINDVPSFDVVERL
                     AVPLPVEMICHILGIDRSEFGRAKRWSDAFAAAAGAEFSSPAERNELMLSTIKEFSTY
                     FVPLIEARRTEPRSDLVSAMVAAIDNESLSNVETLMVAITIMVAGNETSTNLIGNTVV
                     ELLANPDQLELLLDDPSLLPNAVDEANRLTAPIQFAFREATEDTEIAGTTIPKGAILA
                     LHMAAANRDPRRFDEPDRFLITRSAAKNLSFGHGIHFCLGAHLAGQEVRAAIGGLLPY
                     LDDLELTGAPLQRNPTALLNGWQRVELAWRS"
     gene            22988..23941
                     /locus_tag="VGP27_21840"
     CDS             22988..23941
                     /locus_tag="VGP27_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853974.1"
                     /translation="MFTAHVVEVAIATPRGDGLRAAVVRAADQWNAVGSRRHQIVLAP
                     SFGQASTRAAEDLDHADVSISVFDPMQPNLNEAIGDVVHSRRSGTLALVWLSAESPSD
                     IQNADDQARLRQVTQRLACEGVVPRFIGHRDSAAESRVYEAIAADLPYSDLSALIARF
                     ESRAAARRVATYRSPVPLLGPQICAVTVMNHSTFMATGLSVTVDAVDSHGNLLTDGAQ
                     RSTQALADVVAKLRVGPDAPAPTAGPSSPAGRMEILASHTALRYPRWLRPHQRASGLY
                     VLALNASLRVRIEFEDETGASWSRTNDAEPERVREGHWLCP"
     gene            23962..>24322
                     /locus_tag="VGP27_21845"
     CDS             23962..>24322
                     /locus_tag="VGP27_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853975.1"
                     /translation="MPSYEVVGDWADLPGVEGAESCWVHNAVVATDGGEIICFHAGQL
                     VAFDSDGRLLRVVETGLTEGHGITLVREGNAEYLWVSDPGFVFLCGAGDGDPNWTPLF
                     GKGVHCETRAPRVVKMSL"
BASE COUNT         4116 a         8137 c         7822 g         4247 t
ORIGIN      
        1 gtgctggaag cttccgatcg gttgcccaaa ggccttgcgg tccttggcat actgcaatgt
       61 ctggcgccag gtctcgcgcg cgcctgcaat cgcagagatt gcgatggaca gccgctccga
      121 gggcaggttg tgcatcaggt ggtagaagcc gcgtccttct ttgccgagca ggttggcaga
      181 cggcacccga acgttgtcga agttgagctc agcggtgtcc tggtagtgca gccccatctt
      241 ctcgagcttg cgaccacggg tgaaaccctc cataccctcc tcgaccacga gcagggtaaa
      301 gcccttgtgt cctgcgtcgg ggtcggtacg gcacaccacg acgaccaggt cgcagttgat
      361 tccggaggaa atgaatgtct tggcgccgtt gatgatccag tcgtcgccgt cgcgcaccgc
      421 cgtggtgcgt attccggcca gatcgctgcc cgcaccgggt tccgtcatcg cgacggcgac
      481 gatcagctcg ccgctggtga tgcccggcag ccaacgctgc ttctgctcgt cgttggacaa
      541 cgacgagaag tacgggccta cgacatcgtt ctgcaggctg agcgacggcg ccggtccgcc
      601 gtagcgggcg atctcctcgt cgatcacggc gttgaatcgg aagtcctcga cgccccctcc
      661 gccgtattcc tcgggcatgt tgaagccgat caaaccgtgc ttacccgcgg cgatgtacgc
      721 agaccggtcg accagccggt cggcctccca tttctcggcg ttcggaacga gctcctgctc
      781 gatgaactgc cgcaccgtct cgcggaatgc ctcgtggtcg gcctccagga ccagtcgcct
      841 cattggttcc tctcattctc cgtgcaagcg gcgcatctcc tcgccgacct tgcctgacat
      901 tcgcgagacg acctgcgccc cacgccttct cagatcaaca tcgggcacag gcagaactac
      961 cgcaccggtt tccgtggacg gcaacaagac cttcgcgctg cccttggtgg ttgcctcgtc
     1021 gcgctggctg gttgcgaata cgtcgatcga caccgtctcc cctggtccct ggcccttcgc
     1081 agtgacggtg ccggtgcagc ggtgagtgtc gccgtgatag ttgaatccgc gcatctgcag
     1141 gtccatctcg gccagccagc cgtcgtcgcc gatccagttg gtcagtagat ggctcaccca
     1201 ggcagcgcgc atctgaccgt agtcgtaggg cgccggaatg cccagcgcct gagcgcggtt
     1261 gggatcccag tgcagccgct ggaccacgtc cggcacccca tactcgtcgg gttgatagaa
     1321 cgcgggcatc cgcttgcgca gttggtgcgc gaacttcagc gggccgacgc cgtagccgcc
     1381 ccagccccaa cccatgtgca tgctgatgat gtccaccacc gtcagcggcc ccttcatcac
     1441 catcggcagc tcgtcaccga cgttgacgtc ctcccaccac cgcggttcgg cgccgcgccg
     1501 ctcctcggct tcgtaagcgg cctcgacctc ggcgaagtcc tcctccgtcc agtgctggcg
     1561 ttcgatgccc gccagcttgc cggacttctt cgaaccctgc cgctcggcgt tgatatagga
     1621 ctcgtcgcgc gtggcgatcg gattgccgtc ggcgtcgacg tacaggtatc ggtatgtttc
     1681 cttcaccgag cgtccgccgg agaaccggct ctccttgaca tcaacggcga gagtgtaggt
     1741 ctccatcagg actggtcgtc cgacgtaaac aggttggtac catgtccatt tcacgccgga
     1801 gtagtacttt ccggtgcccc ggaacaggcc ccgaaacagc ttcttgcgtt ccgggtcggt
     1861 gaacttcggg gtctggtcca gccccgtgct gatcgggaag gtcggcgaag cgatctggcc
     1921 gtgccagcgg gtcgtgttgc cgtactgcgg gtcgtggaac aacgggttgt cgtcaccgca
     1981 gccgaatgcg aagtgagtga tggcgtccgc gctgggcagc ttgttccatg cctcgtcttt
     2041 ttggttgacg gcgataccga tctgcgcctt tgcgcgttcg atgtcctcgt cggtgatacg
     2101 cccttcgaca gctgcggtat cagcgtcgga tttttctgct gtagtcacga tgtcctcctc
     2161 agaacttcag cgttgcgcct gcatcaatcg tgaactgcag gccggtgaca taccgagact
     2221 catcggaggc caggaagcat accccgttgg tgatgtcctc gggctccaca tatgggatgg
     2281 gcatcgcctg catcaccggc cacgccaaca gcgcatcgtc cctggtcgga ttctccaggt
     2341 cgggccggaa ggtccggtac atcggctcgc tgttgagcat cggcgagttg acgttggtcg
     2401 gatggattac gttggcccgg atagacagcg gcgcaagttg tttcgccagt gctttggtgt
     2461 agcggtcgat gaattgcttg gccaggttgt agccctcgcc gcccggaccc atcggaccca
     2521 ccgatcccgc agccgtctcc gcgatcagac ccgcgacgga cccgatcgtg atgaccgaac
     2581 caccagcctg caggtactgc aatgctgcat gcacggtgtt gaccacgccg acgaagtcga
     2641 cgtcgaaggc gtcgacgaat ccgccgatcg gcacatcgga tccgagtggg cagatgccgg
     2701 cgttggccac tacgacgtcc agtcttccga attctgcgac cgcattggcg atttcacggg
     2761 tcactgccgc gcggtcacgc acatcaacct cggcggtgta cgccttacgc ccggttttct
     2821 ctacttccag tcccgcctct tcgagatccc gtggagtggc cagtggatag tggttgttct
     2881 cgatgtcgtg gcagatgtcg aacaggatga tgtcggcacc ctcttcggcc agtttcaccg
     2941 cgtggctgcg gccctgaccg cgggcaccac cggtcaccag aacgactttg tcctgcacgc
     3001 gtcccatcac gtcacctttc aatcgggttg tgcaacagca gtgagcttct cgagcgtcgc
     3061 aacaagttgg cggcggtcga ccttcagtgc cgcacccctc ggcagctcgt cgacgacgaa
     3121 caccttcgcg ggtacctcgt atggcgtcag cgacgccctg cagtgcgcgc gcaactcgtc
     3181 accggttgcg gtcatcgcgg atctgagttc gactgcggcg accgggatct ggcccagtcg
     3241 atcgtcggcc agtccggcca cggcggcgtc tgcgaccgcg tcgtgtgtgc gcagggcccg
     3301 caccaccgtc tcgggcgcga tcttgaagcc gccccgcacg atgacgtcgt cggcgcggcc
     3361 gtcgatgaag aggtaaccgt cgctgtcgat gtgggcgaga tcgcttgtgg tcacccagcg
     3421 gtcggcggag tcgcccactt gagcagaggc cacctgcaac cgtccggtct gcccgactgg
     3481 tagctccgcg ccatcttcgt cgaccacctg caggcggacg ccggggaagg gatggccgac
     3541 actgcccttc tttgcctccc aagaagcacg aaagtccttg gcagtccagc cggcaacggc
     3601 gcctgcgaac tcggtggcgc cgtaggtgat gagtatcggg atgccgtatt tctgcaagaa
     3661 cgcatccacc atcgccgggt cgacaggcgc ggttcccgag gtgatggcgc gcacgctggc
     3721 cagatcctcg cgcgggatgt cggagttcag caccgcgcga atggcaggcg gtggcaggcc
     3781 taccagaaca ggtcggtgtt ccttgatcgc gttatgccag ccggataccg agaaccgctc
     3841 cagcatcacg atcgggcggc cggccaccag cgactgcaac agcgaccaca gccctccgat
     3901 gtgcacaatc ggcagagtga ccatgccgac ggatcccgtc aggggtggtt tgtcgctgct
     3961 ctgcgggcgg ttgttgtgct gtagcgccgc ggacagtgaa acctccaact gcctgcggcc
     4021 cagggggatc cgcttggcag gcccggtcgt gccggacgtc agcatttcga tggcgattgc
     4081 cggattgccc tggcgtggtt cgtgcgccgc ctttgcgcgg acggccacct cggttccgtc
     4141 gacgctgaat gccgaggcgc cgagttcttc gacggcatcg gcgaacagcg gctcgctcca
     4201 tagatcctgc ggccccagca cgtagtcggc gccggacgcg acgagatctg cgctgagccg
     4261 ctcggcaggc tgcagggggc tgatcgtcac cagcgtgcga ccggcccgga agatcgcgat
     4321 gatcgccgcg accgactccg cgcggttgcc caggacgacg gcaacgcggc cgccaggtcc
     4381 gcaccctgcg gaggagagtt cgcggtcgat ccgctcggtc agctcgcgga cgtgccccca
     4441 ggtcagccac tggccgtcgc gctggatcat ccgagcgtcg tccggggcat tccacagccg
     4501 tcccagcgcc gtttggatgc ttgtcatcgc cacttcctgc gtctgaaggc ccttgccaaa
     4561 ccgtttcgtc aaacgtagtc gacatggttt actaggtcaa cttactgaag ggggtcgcga
     4621 gtggacttcg gcctgaccgg tgagcaggaa cagctcgccg atgcggagcg gtcctggttg
     4681 ggccgaaacg acccgatcga acgtctgcga caggctctcg acaaggctcc gatcaccgtc
     4741 gatccagccg cggttgccca cgcagcggac tctggattgc tggcacttct gaccccggat
     4801 gtcggtggaa cacacgtcga cctcgcggtg tgcagcgagg aacatggata cgcggccagc
     4861 tcgttgcccc tcgcggatat cgcaatcgct tgctggcttc tcgagcgcgc cgggctgccc
     4921 gaggcggaga ccgccgcagg cggcgaactg ctcgtcggcc tcgcccgcgc ggtcgacctg
     4981 gtcacgagcc cagtcccgat ggctgccgag atgaccggct tcgccgtcgt gaggcggacc
     5041 gatgacggtg aattcgtcgc cctggtacgt gagccgaaac tgtcggcaat gacgacactg
     5101 gatctcagcc gatcctgggc ccggctcgac ctcgacggct ctgcgctcga ctgggtggca
     5161 ctgcccgcag gaacactgcg gcatgtgcgc gatgcgctcg cggtacaccg ggccttcgac
     5221 gcgctcggcg gtgcgtcgcg gctgctcgaa atgaccgtct cctatgcgct ccagcgcgaa
     5281 cagttcggcg cggcgatcgg ctcgttccag gccgtcaaac accactgcgc gaacatggcc
     5341 gtcggcgtcg aggccggccg cgcggcgctc tgggcgggag cactgtcgct ggacgaggag
     5401 cccgacgacg cccggtcgcg cgcctgctct gcggccgtcg cgtacgcgaa gtcggcggcc
     5461 agtgaggtcg cgggcaccgc gctgcaggtg cacggcggca ttggattcac ctgggagcac
     5521 gacctgcatc tgttcctgcg ccggatcaag gtcgacgagg ccgtcgacgg aacggtggcc
     5581 gagcaccgcg ccgcactggt cgccgtctag ccagaactac ttgcgcggaa ggcccaaacc
     5641 gcgctcggcg atgaggttgc gcatgatctc cgaactgccg ccggcgatgg tgtaggcgcg
     5701 ggcgtacaac cactcgtcct gccagcggcc gtcgtcgaga acgttgtcgt cgccctcgac
     5761 cagcacaccc gcctcgccgc tcaactcgac cgcgaattgc gccatctcca agttgagttc
     5821 gctgaacatc agcttgccca tcgccgcgtc gtgcatgcgt tcgccgcccc gcagccagcg
     5881 ggtcaggttg gcgtaggtca acgccgacaa cgcctccacc tcggcagtga actccccgat
     5941 tcggtcgcga acccgatcgc tgtcgatcgc cggacgaccg ccgacggtga tccggcgcgc
     6001 gagatcgacc agcgcatcga ccgcgagccg aagcttcacc acgatcccgc ccacgttggt
     6061 gcgttcgtgc gcgagcgtgg cgttcgcgat cgcccagccc tggcctggtg ctccgatcat
     6121 cgcgtccgcc ggcacctcga caccgtcgaa gaacacttcg ttgaagtcgg acgtaccagt
     6181 cagctcgcgc agcggccgaa cactcacacc cggcgcggac atatccagga tgaacgcgct
     6241 catgcccttg tgcttcggtg cgtcggagtc ggtgcgcgcc aacagatagc cataccgcgc
     6301 ccagtgcccg tcggtcgtcc acaccttctg accgtcgatg gtgaatgtgt cgccgtcgcg
     6361 cacggcgcgg gtgcgcagcg aggccagatc gctgcccgca ccgggctcgc tgaacagctg
     6421 gcaccacagt tggcggcccg cgcgaatctc gggcaggtgc cgtcgacgct gttcgtctgt
     6481 gccgtaatgg atcagcgcgt gtgaggccag cacgcttccg ctctgcgcgc cgggagcctg
     6541 cgcgcgggcg agctcctcgc cgacgacgat cgcgtgttcg ggcgaccagt cttttcggcc
     6601 gccgaactcg ggcggccagt ctgatcccgc gtagcccgcc tcgtagagac gccctgtcca
     6661 ctcctgcaac gccttgaact cggcatcgtt ttcagcgctg cgcacgccgg cacggggcgg
     6721 gatcgcaggc gcatgctcag cgatgaacgc ccgcacctcg gcgcgaaact ccgccaactc
     6781 cgggcccatg ccgtattcca cgattctcga cctctatcga ctcaggaccg cacccgcggc
     6841 ccggtcacgc agcgcgtccg cgcggccgac cagcacggcg ttggccgcgg cgcggcgcaa
     6901 cagcagatga acgggatgct cccaggtgaa gccgaggccg ccgtggatct gcagcgcgcc
     6961 ctcggtggcc gacaccgcgg catcgagcgc tgcggccgcg gcgaggtcga ccaggtactc
     7021 cgcttcgtcc gcgtcggcct gcgcggcggc cagcacctgg ctgcgggcgc cttccagcgc
     7081 caccagcatg tcggcgcagc ggtgcgagat cgcctggaag ctgccgatcg ggcggccgaa
     7141 ctgctgacgt tcgccggccc actccaccga catcgccagt gccagcgaag ccactccgac
     7201 cgagtccgcc gccaccgcga gcagtgcctg gcggcgggcg tcgcgcagcc cgttacgcaa
     7261 gtctgccccg tcggacagca cttccccggc ggccgcggtg aaggtcagcg cgccaacggt
     7321 cgcggtcagg tcgagcggct gctgaggtgt cacctcgacg ccgtcgccgg cagggtcgac
     7381 cgcgacgaga gcctcgccgt ccccggtcgt ccgcgcgagt accaacaaca cctcggcgct
     7441 gggtgctccc gcgacgatcg ggaccgagcc gctcagccgc tccccgtccc actcgggcag
     7501 ttgcggcccc gctgtcagcc agccggagtc gtcgagcgga accgcgaatg ccgcgggggc
     7561 gcccgccgcg atgcgctgtg ccgcctcctt ggctccgacc gcgaccgcca caccgagcgc
     7621 cgtcacggtt ggaacgagcg gcaccggcgc aagcgccttg cccgcctcct cgatggccgc
     7681 gtagagcagt tgtgggcgcg caccctcgcc gccgagtgcc tcgtcgacgg ccagtccggg
     7741 cagcccgaac tcgacgagcg cggaccaggc ggcggaatcg caggcatcgt gggctcgctt
     7801 ggggtcgagc agcgtttcga cgtcgacccg agccctcagc gtctgcgaga gcacgtcgcg
     7861 cagttcgcgt tcgtcctcgt cggcgacggc gagtggggcc cctggcacga cagcggcggc
     7921 ccggctgccc tcgccgcgat gcgatggcag gccgagcacg ctttcggcga tggtgttgcg
     7981 ctgaatctcg gcggtgcctg cgccgatggt ggtggctcga ctcatcaggt acccgaatgt
     8041 ccagcggccc ttgtcgattg cggcggctgc ccgactgccc agcgccgcgt gcggtccgac
     8101 ggcacgcagt gcaagtgcat gcatgtgctg ctcgaattcc gatttcacca ggcggttcac
     8161 cgaagcgacg ccgccgggat cctctgcgcg gagcaccgcg gccagcgcac gcgcgctgtt
     8221 ggccgcgatg gtgcgcacgc ccgattcgag acgggcgatg tcctggcgca cctgcgggtc
     8281 ggcgtccagt tgtccagacg ccaccagctc gatgaccctg tcggcggtct ttcggtattt
     8341 gaactcgtcg gccagaaacg cggtggcccg ctcgtggccg agcgaggtgc gcatgatcga
     8401 ccagccgcgc ccctcgtcgc cgacgcggtt ctcgacggcc acctcgacgt cgtcaaggaa
     8461 cacctcggcg aagtgggccg cgccgctgat gtcccgcagc ggccgaaccg tgatcccgga
     8521 actgtccatc ggtatcagca gataggtgat gccctcggtg ctgcggccgg gtgggccggt
     8581 gcgcaccagc gcgaacatcc agtcggcccg atcggccatc gtcgtccaca ccttctgacc
     8641 cgtgacccgg tagacgttcc cgtcacggac ggcgcgagtg gacagcgacg cgaggtcgct
     8701 gcccgcaccc ggttcggaga agccctgaca ccagaactcg tcggcacgca acagtggcgt
     8761 gaggaaccgg gccttctgct cggccgtccc gtgctcgatc agcgtgggcg cgaggatgaa
     8821 cgacagcggg cacgggtgcg gaggcgcccc cgcggccgtg gtgagccggt agtagtcgag
     8881 ctgatcttcc agcgacaact ccaggccacc gaccgacttc ggccagcccg gagcggcgag
     8941 ctcgttgtcg acgagcctgg catgccatgc gcgcgcggac tctatgcggt tcggcttgcc
     9001 ggtctcggcc cgctggtcgc gccgataccc ggcgagcagc ccctcgagtc gggatcgcca
     9061 gtcggctgac ggcatctgca ccccacaaag ttaacttggt tacttatgtg agcagtctgc
     9121 tgtgtcgggc gggcagttgg caagtgctgc ggtcagtcct cttcgagaat cagggccaac
     9181 tccgggcagg ccgcaacacc gtcacgcgtt tgctgctcgt ctccgggctt gacgtcgtga
     9241 gcctcgagga tgctgtagcc ggaatcgtcg atcggaaaca ggtccggatc caccgcataa
     9301 cactgcgcat gaccgacgca ctttgcctgt tcgagacgaa ctttcaccgc aactccttcc
     9361 gagttaacac gccaccctta tcgagaaatc ttcggtggca cgtgcgctcg ggttctcgac
     9421 gttgaaaccc gttgcgctgc cggtcaccac aaacgtctgc ccggtcatgt cgaccttcgc
     9481 gtcaggcccg agatgcgccc agtaactgcc ggtgaaaccc ccgacatcgg tgatctgagc
     9541 gaattgcacc gccagtccgt cgcgggtgtc gatggtcgaa gtggtgcccg aactctcgtt
     9601 cccggtattg atcgtggtca ccggtccgac ggttgagcaa ttgacagcat tcgacctgcc
     9661 cgcacctgcg ccgttgatac tcacttccgc ggtgcccgcc ggcaggccgc ccgccggtcg
     9721 tgcagcgggg gccgggtccg agcagccggc gacaccgcag gcaactacca acgcagacgc
     9781 gccggcgatg aatcggttat tcacgatcac ctctgttccc ctgccgctga tccgtcggca
     9841 cattgcgacg aatgtaacgg gagcgacgtc acgcgtcagg gtaatccgct aaatatctcc
     9901 ctcattcggc ccggggtacc ccggtcaggc tacggtcgac cgcgggaagt tcagcatcct
     9961 gcgcgcgttc tcctcgacta tcagggagca ctcgtcgtcg ggcacgccgg cgagcacctc
    10021 ggccgcccgc ttgcgggagt tgggccagtt ggagtcgctg tgcgggaagt cgatctccag
    10081 catgatgcgg tcgatgccga cgctgtgccg gttctcgagg ccatgttcgt cgtcgatgaa
    10141 gcagccccag aaatgcttgc ggaacagatc ggatggccgg gcatcgaagt cgatcggctg
    10201 gtagtagcgg tgccgctccc acacatagtc ggcccgctcg aggatgtagg gcacccaacc
    10261 gatgccgccc tcggccaggg agatctgcag atcgggaaac tgctgcagtt ttccggagaa
    10321 cagccagtcg acggtcgacc acatgaggtt cgtcgaaaac agggtgatcg caacagcgaa
    10381 cggcgcgtcc ggcatctgca tctggccggc aaccggcgcc atcgaggaga acgagaagcc
    10441 gggaacgtag gaccccgagc cgaaatgcag cgacagcggc atgtgcgcat ccgagcagac
    10501 ccgccacagc gggtcccagt gatcggaatg gaacgacggc aggcccagcg gcacggggct
    10561 gtccgggaac gacacggtgc gcgcgcccat ggcggccacg cgctcggctt cgtcgacgca
    10621 tgccttgacg tcccaggtcg gcaggatggc cagtggcaca taccggtcgg gcgcggtggc
    10681 gcaccattcc tcgatgtaga agtcgttcca cgccttcaca caaagcagcg ccaggtcctt
    10741 gtcggcggcg cggtgaaagg ttccaccgcc gaatccggga aaggacggga agcagagcgc
    10801 ggtctgcaca ccgtcgagat ccatgtcggc gacgcgggcc accgggtcgt aacagcccgg
    10861 gatcatgtct tcgtaccgca cggggtccat gccccactcc tccggcggct tgcctgcgac
    10921 ggcgttgagg ccgatggtcg ggaagatctg tccgtcgtag gaccagaggt gcttgccgtc
    10981 catttcaacg atccgcgggc cgtgctcacg ccacttcgcc ggcagtcggt cctgccacac
    11041 ccgtgggtgt tcgacgagat ggtcgtcgac cgacacgatc tggtggtggt cccgaagcgg
    11101 catggctgca ctcacatttc tgacaacttg tcttactttc tgacacttcg tctcagacgg
    11161 tatatgatgc aggccacagt tgtccagggt tgagaggtgg atcatgtcgg cgaccgaaca
    11221 cacgccggat gcggaggcac gcggcaagct cgacgaggac gaccacgacc tgctcacctt
    11281 cggagaagca ggcgaacggc ttcgcctcga gatcaccgcg gcccacgacg cggtcacccg
    11341 actcagtgag tgcggtgata ccgctggcct ggagaaggcc cgccaccgac tggcggcgtt
    11401 gcgcgccgcc gccgaccgca attcggcgca acccatcaac gacgccaact tcgagaaatt
    11461 cttcggctac cccggcaagg ccaagcgcaa cctgccgcag gcgccggccg cagaatgacc
    11521 actgccgccg acaagttgcg acggccggga tatgcgcccc cggccaacgc cgtcgtcggt
    11581 cggcgcggcc tccacacccg cgaccggatc gtgtcgtgtg cggcagaagt cttcctgcag
    11641 aacggctttc acagtacgtc gatcgacacg atcgcgaagt cggcagccgc gtcgcgggcc
    11701 accgtctatc agtatttcgc cgacaaggaa gcgatcttcg ccgaactgtc ccgcgctgcg
    11761 gtgcgatccg tgctcgggct cggcgagcgc ctcgggccgc tggggcccac cgcggacgga
    11821 ctggccaacc tcgaggcctg gatgcatgag tgggcggaca tctatgacgc acacgcggcg
    11881 gtattcgccg agtaccccgg catcggcacc gtgatgggac tgacggcggt cgacgcccat
    11941 tcggtgccgg aaaggttcac ccggcacgtc accgatcgac tccgctcggc cccggttcgc
    12001 ggactggaaa tcgccgacgc agtagcagcg ctcatgcgga tcccccacat ggtcaatctc
    12061 tatcgccacc gtgggctgtt cggactgcca tcccggcaga ccgtatcggg ctcactggcg
    12121 atcgcactcc agctcatgct tttccccgac acggcggaca caatgacgtt cgccgcggtc
    12181 gcgcctggcc ttgacgtggc cgtaccggtc gcgcacgacg ggcgcaaacc gtcgcctatt
    12241 caccaggacg tgctctctgt cagctcacag ttgttcgcgc agcgcggcta ttacgcggtc
    12301 gggatggcgg acatcgccgc ggcggccgac atcagccggg cgacgctgta tcggtacttc
    12361 agcaccaagg acatgatcct tgcggagctg acccgcgcgg cggtgctcga agccgagcac
    12421 cacgccgcgg cattgaagct gttgacgatc aatggcgttg atgccgagca gttttcgcgc
    12481 tggactcatg actacgtccg atttcaccgg gcctacagcg gcgtcatccg tgcctggttc
    12541 gacggcaccg tggccgaacg cctgtccgaa gcggcggccg gcgacgggat cgcatcgata
    12601 cgcgatgcgg tagccgcgct actccaaacc gcgacgcttc cggccggggt ggatcccgcg
    12661 acggcgcagg cagtgttcct ggcgatcctc gggcggatga ccgagccgac tgcgtcgggg
    12721 cagagcgacg cgcatgcggc cgaggtcatc gtcgcgttgt tgggccgctc gctgctgagt
    12781 cagctcggcg ccgcgacgta accggcagcg gagttgcgta actgccagat ctgcgccggg
    12841 cacaattcgt tggcggcctg actgatcaga taggaagcct ggaactcgtc gttggtattg
    12901 aagtcaccct tgatatcgcc caccagctgc gcgtacggcc tgccctgggc gatcttgtcg
    12961 cagaccccat ggccatacgc gagggcggcg tcggcgttgg cgaagttgta tcccggcttc
    13021 accgtcacgt tcaccagata cgcaaccgcg tcggcggacg ccgtcggcgt gcctgcgatg
    13081 accccgcccg tgcagaacag cgcggcagag gcgaggctga tgagcggata cgtcttcata
    13141 gaaccaaaag cgtaattcat ttagcttatt accgggtaat ctcttcgcag actggggagg
    13201 cacgcatgac acgcagcaag gctggtcgat tcggactgac gataccgttg ctggtcgtcg
    13261 tcgcgagtgc gactgtgatt ccgacggcgc acgccgacaa caagcggctg aacgagagcg
    13321 tgttcgtcaa catcgacacc gcacagaaac agaacggttg tccgggcgag gcgcacctgg
    13381 acggcaggct cgtagaggcg gctcgcctgc acacactcga cgtgctcaac aaccgggccc
    13441 tcgacgggga catcggctcg gacggctcga ccccgcagga ccgggccaat gcgcagggct
    13501 tcgtcggcaa ggcctccgag accgtggcga tcaacccggc acttgcgatc agcgggatcg
    13561 aaatcctcgg ccaatggtgg tacaacccgg cttacaaggc gatcatgcag gattgctcga
    13621 acctcgccat cggcgtgtgg tcggagaaca gcctcgaccg cacggtggtc gtcgctgtat
    13681 acggccagcc ggcctaaccg gccgccgccc ctgcaacatc cgcgcggagt cgcttcttgt
    13741 cgaccttgcc gctcgccaga accggtaatt cggcgacaaa cacgagtcgc cgcggcacct
    13801 tgtaacccgc gagcctggac cggacgaagt cctggaccgc ctccagcgtg agggtggcgc
    13861 cggccgtcgg caccacaacg gcgcagaccg cctcgcccca tttctcgtcg ggcgcgccga
    13921 tcaccgcgca ggccgcgacc gcgtcgtgcg cactgaggac gtcctccacc tccggtgaga
    13981 acacgttctc cccgccggtg atgatgacgt ccttcttgcg gtcgactaag tacagcagcc
    14041 cgcggccgtc gcatcggccg acatcgccgg tgcggcacca cctgtcccga agcgtcgcaa
    14101 gcgtcgccgc cggatcgtcc cagtacccgc ggaacaccga gtctgagcgc acgacgatct
    14161 caccggcctc gccgaccggc acatcgcagc cgtcgtcccc gacgatccgc acccgtacac
    14221 cggggaaggg gaacccgacg gagcgcagtg cctcgcccgc atccggaccg tcgaggacgt
    14281 gcagttcgcg cggcaacccg gacacgatcg cttcggtctg gccgtacaag ttcagaaatc
    14341 ccgcgctggg cagcgccgac atcgcgcgcg caaggacgtc ggccgttatc ggcgcggccg
    14401 aatagatgac ggtgcgcaca gtctcgaggt cgcgcgcatc ggtcactaca tcgagcaacg
    14461 cccgcagcat caccggtgcc aggtgcagca cggtgatgtt gtgctcggcg atgattcgca
    14521 cggcctcggc ggtgtcgaac tgctgctgga gcaccactgt tccgccgcga gcgtgcagtc
    14581 cgccgatgat cgccatcgca ccgacgtgaa acatcggcat gttgatcagt ccccggtctg
    14641 cactgccgga ccgcatttcg acgttcatgg tgaacgccac ccgcctcagc tcccgctgcc
    14701 ccaggatgca gcatttcgac gccccggtgg tgccgctggt gaacagcagg caggcgatgt
    14761 cgtgggggcg cgcggcgaac gcgagctgcc cgcaatcgcc ggaggtgatg aactcctcga
    14821 aggcgaccat gtcggagcga gccgttcctc cgatgcacac cagtagcggc atggacggca
    14881 atcgcggcgc gagttcggtg atcacggcgg cgaattcgtc ctcgcagaac acgacggatg
    14941 gggcgactcg gtccaacacg tcgagtacct cgagtgcgga aagccggaag ttgaccgtcg
    15001 ccatgatgat cccgctgagc tggctcgcgg ccatcacctc accgaactcg atgctgttgc
    15061 ggctcagtac ggcgacgcgg tcctgccgtc gcacgccgac tgctgccaac gccgacacca
    15121 accgcaccgc acgatcacgc agctgcgcat gggtgaccat ccgcgaaccc gaccggtatg
    15181 cggggacatc cgggaaacgc accgcgttgt cggtgacgac atcgccaaga gtcatgtcga
    15241 ggtcgtctcc ggtcacgggc tttacctgct actcaacaac atcgaaccgg ccaccggccc
    15301 gccgccgacg ccgacagcga ccacttccgg tgtctgcggc acctgccggt cgccgccctc
    15361 gccccacatc tggacgcagg cttcgtgcag gaagcccatg ccgtgcaggc gaccgcccga
    15421 gagttgaccg ccgctggtgt tgatcggcag cgagccgtcg agcgcgatcc gttcaccgcc
    15481 ttcgatgaat tcaccgacct tgccgtgctc gcagaatccc agtgcctcga gccacatgac
    15541 ggtcaagaaa gagaaaccgt catagatctg ggccatgtcc acgtcggcgg gcgtcaacgc
    15601 ggtgttctgc cacagtgtcg cggcggaatc gtgggcggcc atcgtggtga ggtctgatcg
    15661 ctggtcccat gtcgcgcgct cgaacatgcc cggcccgacg gactcgacgg tcagcggatg
    15721 gcgcggcaga ccgctggcgg cgtcgcgacg ggagatgatg accgccgtcg caccgtcaca
    15781 gggagcatcg cagtcgtata ggcatagcgg ttccgagatc atgcgggcac tcagatagtc
    15841 gtccatcgtc atcggctggc ggtagacggc gtccgggttg agcccggcgt ttcgcctcgc
    15901 gttcagcgcg atccagccga gttgctcacg ggtcaaaccg aagtcgtgca tgtatcgctg
    15961 ggcgggcatg gccaaccagt tcgtcgccga caaggctccg aacggcgcga cccattccaa
    16021 ttgcggcggc agcttgcccc cggtgaaaag cacggaggca cgtccgccgc cggcctgctc
    16081 cgcggccgtg gactcccaca ccgaccggaa caccacgacg tgattggcca atccgagggt
    16141 gacggccatg cacgcttcga tcgccggacc gatctgaccc gcggtctcga gcccggacat
    16201 gtaccaccgg ctgcgcagcc ccagcacatt gcggacgtcc tggacactcg cgccggaaaa
    16261 acccagatcg ggaactccgg gtcccgggta gctggcgatg ccgtccacgt cgtcgacggc
    16321 gagcccggcg tgctcgattg cccgcaacac cgcctcgacc gtcaggtcca gacccgtgcg
    16381 gcccagccgg cgcccgactt gagacttgcc tgcgccggtc agtacggcct ttccatcgaa
    16441 cgggcccctg gtcacgttgg cgagggtagt catcagaaga tgaaccgggc gatgagttcg
    16501 gcgacggcgg cgggtttgtc cacgccgtcg atctccacgg tgtgccggac ggtcaggttg
    16561 acggtgtcgt cggcgacctt ggtggcgtcc gcgagttcag accgcacccg gacgcgactg
    16621 cctgcggtga ccggggccag gaatctgacc ttgttcagtc cgtagttgat gcccatcttc
    16681 gcgttttcga cgcggtagtt gtccttgctc acgccaggga tgagcgacag cgtgaggaag
    16741 ccgtgcgcaa tcgttgcgcc gtagggactc tcggccttgg ccttctcgac gtcgacgtgg
    16801 atccactggt gatccatcgt cacttcggcg aaggcgtcga tacggctctg gtcgatgtcc
    16861 acccagcgac tgacgccgag ctcggcgccg atcaacgcga tcgcgctgtc gatggatccg
    16921 attgtgtgca cctgctgctc ccgtcgccgc cacccgaaat accgatatct atttaacggt
    16981 atagactggc cccgcccgca gggcaaccag ccccggagtc cgacgatctt gggagacaca
    17041 tggccaccac tacatctcgc gcagcgatcg tggccgctgc ccgcacaccg atcggaacgg
    17101 cgcgcagggg cacactggcc aacgtcccgg cgatcgaact ggcgaaaccc gtggtaaccg
    17161 ctgtcgtgga gcgctccgga ctggatgccg ccgacttcga cgatttcgtc ctggccgaga
    17221 gcatgcaggg cggtggcgac agtgcgcgct acatcgcggt ggacctcggc ctgaccgaca
    17281 ttcccggcat cgccgtcaac cggcagtgcg cgtccagcct gtcggcgatc gcggtgggtg
    17341 cagggcagat cgcagccggg atgagccgcg ccatcctggc cggcggcatg gagtcgctgt
    17401 ccaccggacc gatcatgcag aagcgcaagc ccttcacgtc cggcaagtct cccgaggact
    17461 atgcgcagtg gtttcccgag tcgcatcctc cgacagccga agcgcctgcc atggacatgt
    17521 ccatcactgt cgcgcacaac tgcaacgtcc agtacgggat cacccgcgag gaccaggacg
    17581 agtgggcgct gcgcagccat cagcgtgcca tcatggccat cgacgccggt tcgttctccg
    17641 acgagatcat cccgatccag gtgccccagg ccgacgggac ggcgatcacc ttcgccgagg
    17701 acgagcatcc acggcgcggc agttcgatgg agtcgctggc cggactgaag gtgctgcacc
    17761 cagagatcga gggcttcacg gtgacggcgg gcaactcgtc gggcatcaac gacgctgcgg
    17821 cggtcgtcgc gctggcgtca cccgacaccc agcagaatgc gctggccaac gtgctgtcct
    17881 ggacgcaggt gggactcgac ccgacccgca cgggtagcgg accgatcaag gccatcccca
    17941 aggcgctgga actggccggg cgcaagctcg aggacgtcgc actgttcgag atcaacgagg
    18001 cgttcgccgc gcaggctgtg gcgtgtgcgc gcgaactcgg actcgacgag gagatcgtca
    18061 acgtctacgg ctcgggcatc agcctcggtc atccgatcgc tgcgacgggc gcgcggatgg
    18121 tgacgtcggc gatctacgaa ctgcgcaggc gcggtggcgg tatcggtgtg ctgtccatgt
    18181 gtgcaggcgg cggtatgggc gcggcaatgg tcatcgaggt ggtctgacat gaccgcactg
    18241 atcaccgagg ggctgttccg agtcgacggt gagcgggccg tgttgttcgg atcgcggcgg
    18301 cgcagcaccg gtgtcgtcaa gttcccggcg gagcgcccag agttgttcga cggtagcccg
    18361 gatgtccagg ccgacatcga gcctcttgaa ctgtccacgg agggaacgct ttacacattc
    18421 accacccagg agttcgctcc gcccttgccg tacaagggca accgggaccc gaaagtgttc
    18481 aagccctacg tggtgggctt cgtcgagttg ccggagggct tgctcgtgga gtcgctgatc
    18541 atcgacgccg ccgcgagcga cctgcggatc ggccagcggc tggtttctgc caccaccacg
    18601 ctggacaccg cggacggcga atcgttgctc actttcgcgt tccgcgccgc ttagcatcga
    18661 tccggtgacc gacctcggca cgccggagtt cgcgctacat ccgctgcacg gcatccatca
    18721 gcccaccacc gggaatccgg cgcggcgccc cggctccgcg cgccggactt cgtccatcga
    18781 tatgacccgt gacgagggct cgctggaccc cgtctatctg caggggcgag cgcgcgatct
    18841 gtggacggcg gccgacgggt cggtgtcaga agtcggtgcc gcagaactct cggccaccat
    18901 cgagctcgtc gcccgcgtcg tccgacacgt cgaggtagtc ccgccggtcg cgggcgtggc
    18961 cggtctctcg ggcgcgccgg cgatgagcgg gttccgcgcc gctgccgaca aggccgcccc
    19021 agagcttcgg cagcaccgcg acctccggta cacgcttctc gacgacgttc cggtcgcaac
    19081 gctgatctcg ggtcattcgc tgtcggcctc gaacgtgctg gcgcacttca cgaaatcggg
    19141 ctatctacct gtcgccgacc agtgcgccgg attcgcgacc ggcggcctgc tgatgacatc
    19201 gttcgaagcc ggcgaccccg tgatcgtcac cggtccggaa gctcctgatc tcgatcacga
    19261 cagcgatcga ctcgcgtggc acccgatggc gcagttgccg cgtcatggca tgcgcaggcg
    19321 gcgtcgcatc gacgtcttcg atgccggtga agacagccga gtcggcatcg atgcgatgtt
    19381 ccgcgatacc tacgtgcgtg ccgacggcgc ggagaccatc atccatgaat acaccctcgc
    19441 ggctgcggtc gacgccgaca ccggcgtcat cgtcgaatca cacgcgacgc cgcgggtttt
    19501 gccctggcag gaatgtcccg gcgcggtcgc cagcgccgag cggatcaccg gaatgacgtt
    19561 gggcgagctg cacttccggg tgcggcagga actgttcggg accagtacgt gcacccacct
    19621 caacgatctg ctgcgcagcg tcgctgacgc cgaggtgctg atcggccttt gccgcggcac
    19681 ctagtcttct ctaggtggcc cgtggtcgcc ggcagcggtc cgttctgggc gaaaaggatc
    19741 agccggccct catttaataa gcagacctaa cattttcgta tacgctgtgt tcttccacgt
    19801 tcgagtggca gcgaagggac tccgatggcc agaacagacg atgacacttg ggacatcaat
    19861 gagagcgtcg gcgcgaccgc cttgggggtc gccggcggtc gggcagccga gaccgacagc
    19921 gctgatccgc tgatcagcga tccgttcgca aaactctttc tcgacgcggc gggcgacggc
    19981 atctggcgga tctacctcga cgccgaattg cctgccgagc tggtcgatct cgatccggaa
    20041 ctaccggtcc gcatgcaggc aatgatgggc tatacggcct gccggacgaa gttcttcgac
    20101 gagtactttg tcgcggcggc cggcgacggg atccgccagg ccgtgatcct cgccgccgga
    20161 ctggacgccc gtgcctggcg actcgcatgg cccgccggct gtgtggtcta cgagatcgac
    20221 cagcccaagg tgttggcttt caagaccagc accttggaat cccaccaggc caccccgatc
    20281 gcatcgcatg tcagcgtcgg catcgatctg cgtctggact ggccaaaagc attggcgaag
    20341 tccgggttcg acccatcggt tccgacggcg tggttggcag aaggcctgct gccatatttg
    20401 actgcggatg cccaagacgt gctgttcgac cgcattcagt cgctcagcgc cccgggaagt
    20461 cgcgtcgctg tggaggcctt caccaacgac ttcttcagcc aggagagctt cgcgcgtcgt
    20521 gaagagcaga tggatcgcta tcgggcagcg gcgatcaaac tcggcggcag ggacatcacc
    20581 gaatccggca acttgctgta tgaggaggaa cgcacggagg tcgtggtctg gctgcgcgcg
    20641 catgactggg ctgtcaccac agcgacgagc gcaaccgacc tcatggccgc caacaatcgg
    20701 gccgtcccgc ccggcctcga tgacgccgtc ccggaaagtg tattcgtcga agctatttcg
    20761 ggggcatcct gatcccgccg tccacgcgga taacttcggc attcatgtac gagttggtca
    20821 ccaattcgac gaccatggac gccaactctt cggggttgcc caagcggtgt ggaaatagca
    20881 cggactcgcc cagcttcgcc ttgaatgcct ccgacttctc gccttcaccg tagatgggag
    20941 tatcgatcag accgggtgcg atggtgttga cccggatccc gaccgctgag aggtcacgcg
    21001 cgaccggcag cgtcatgccc acgacaccgc ccttggacga cgagtaggcg gcctgcccga
    21061 tctggccgtc gaaggcggca acactggcca tcgacacgat cgctccacgc tctccgctgt
    21121 ccagtggctc gttgcggctc atcgccgtcg cggccagccg aatgcagtca aaggtgccga
    21181 tcagattgac ggcgatgacc ttcttatagg cgtcgaggtt gtgcgccgac gcgaactggc
    21241 catcctttcc aatcgtgcgc tgtgccccac cgatgccggc ggagttgacc aagacgcgca
    21301 gcgggccgag ttgagttgcc ctgttgacgg ccgccgcgat ctgctcggta tcggtgacgt
    21361 cgacggccac gaacacgccg ccgatatcgt cggcaagtgc agtcccgcgg tctgcctgca
    21421 catcggcgac gacgaccctc gcccccctcg cggccaactg ccgtgcagat gccgcgccga
    21481 taccggacgc gccaccggtg actattgcgc tggctccgtt gatatccact acgacctcca
    21541 cgccagttcg acgcgttgcc acccattcag cagtgccgtc ggattgcgtt gcagcggagc
    21601 gccagtcagt tccaggtcat cgagatatgg cagcagtcca ccgatcgctg cccgcacttc
    21661 ctgaccggca aggtgggcac caagacagaa atggatcccg tgtccgaacg agagattctt
    21721 ggcggccgac cgagtgatga gaaagcggtc gggctcgtcg aagcgtcggg gatcgcggtt
    21781 tgcggcggcc atgtgcagcg ccaggatggc ccccttcgga atcgtcgtgc ccgcgatctc
    21841 ggtgtcttcc gttgcctcgc gaaacgcgaa ctgtatcggc gcagtcagcc ggttcgcctc
    21901 atcgactgcg ttgggcagca aggacggatc gtcgagtagc agttcgagct ggtcggggtt
    21961 ggccagcagt tcgaccacgg tgttgccgat gaggttcgtg gaggtctcgt tgcctgcgac
    22021 catgatcgtg atcgccacca tcagcgtttc gacgttgctc aacgactcgt tgtcgatggc
    22081 cgccaccatc gcactgacta gatcactcct cggctcagtg cgccgcgcct cgatcagcgg
    22141 cacgaagtac gtgctgaact ccttgatggt gctcagcatc aactcgttac gttcggcggg
    22201 actgctgaac tcggcgccgg cggccgcagc gaacgcatcg gaccagcgtt tagcccgccc
    22261 gaactcactt cggtcgatgc cgaggatgtg acagatcatc tccaccggca gcggtacggc
    22321 cagccgctcg acgacatcga agctgggcac gtcgttgata ccggcgaggc agcgctcgac
    22381 gatcgaactg atcgcgttac ccatctcctt gatccgaggc ggggtgaacc ctcggctgac
    22441 gattctgcga atacgcgtgt gctggggcgg gtccgcggcg gcaatcgtct tggcggaggc
    22501 catctcgttc atgtcgacgg ggatcccggc tgccagggcc atgagtcgtt cgatgacttc
    22561 gggtttcatc tcggcatccg acgccgccga ttcaccctcg tacgggttgg ggcgcgatga
    22621 gaacacatcg ggggtcgact gcaccgtctt cacgtcgtcg aaccgcgata tcacccagat
    22681 gttcgaccgc gtccgatata cggggaagcg ttcgcggagc tgggcgtaaa ccgtcggcaa
    22741 ctgttcgtgc ggaccgggct tgaacggctc gaaatccaac ccacaccggt cggccgacac
    22801 agtctctcct gttcgttggc gaacgtaagc tgtccgcagc acctcgagcg gcaacgtaac
    22861 ggacaaggta tgtgcgcaat agtaggatgt cgtttgcgta aaaagcaagt atcggtacac
    22921 tttgctagca cctcatgtaa tcagacgcga ccactaggtg cagaagaaca cacgaggaga
    22981 tgagccagtg ttcaccgctc atgtcgtgga ggtggcgata gccacacccc gcggcgacgg
    23041 gttgcgcgcc gctgtcgttc gcgcagccga tcaatggaac gccgtcgggt cgcggcggca
    23101 tcaaatcgtg ctcgcgccct cattcgggca ggcctccacc cgggctgccg aggatctgga
    23161 ccacgctgat gtgtcgatct cagttttcga cccgatgcag ccaaatctga atgaagcgat
    23221 aggcgacgtc gtgcactcga ggaggtccgg cacgttggcc ttggtgtggc tatccgctga
    23281 atcgccttcc gacatccaaa acgcggacga tcaagccagg ctcaggcaag tgacgcagag
    23341 gctggcatgc gaaggagtgg ttccacgttt catcggtcac agagattctg ccgccgagag
    23401 ccgcgtgtac gaggcgattg cagcggactt gccctactcc gatctcagcg cgctgatcgc
    23461 gcggttcgaa agtcgcgcgg ctgcgcgccg agtcgcgacg taccgaagcc cggtgccctt
    23521 gcttggcccg cagatctgcg ctgtgaccgt gatgaatcac agcaccttca tggcgaccgg
    23581 cctttcggtg acggtggatg ccgtcgactc ccatggcaat cttctgaccg acggggcgca
    23641 acgttcaacg caggcgctcg ccgatgtcgt cgccaagttg agagtcggtc cggatgcccc
    23701 ggcaccgact gccgggccct catccccggc cggccggatg gagattctcg cctcgcacac
    23761 ggcgctgcgc tacccgcgct ggttgaggcc gcatcaacgt gcgtctggtc tttatgtctt
    23821 ggcgctcaac gcatctcttc gggtgcgcat cgagttcgag gacgaaacgg gagcatcgtg
    23881 gtcgaggacg aacgacgccg agcccgagag agtcagggaa ggccactggt tgtgtccata
    23941 gatatcccta gacccgccaa catgccgtcc tatgaggtgg tcggcgactg ggcagatctg
    24001 cccggcgtcg agggcgccga atcctgctgg gtgcacaacg ctgtcgtcgc aaccgacggc
    24061 ggagaaatca tctgttttca cgcaggtcaa ctcgttgcgt tcgattccga tggccggctc
    24121 ttgcgtgtgg tcgagacggg cctcacggaa ggccacggca tcaccctcgt ccgcgaaggc
    24181 aacgccgaat acctctgggt cagcgatccc ggattcgtct tcctctgcgg tgccggcgac
    24241 ggcgacccga attggactcc gctgttcggc aagggtgtgc actgcgaaac aagggcgcct
    24301 cgggtggtga agatgagcct cg
//
LOCUS       DASYLX010000933         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2799355,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000933 DASYLX010000000
VERSION     DASYLX010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2799355"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGP27_21850"
     CDS             <1..542
                     /locus_tag="VGP27_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7853976.1"
                     /translation="RDEIDEVAQRVYTEVNNGVYRCGFAGSQEAYDKAYDRLFTALDW
                     LNERLENQRYLVGDTITEADVRLFTTLARFDPVYHGHFKCNRSKLDEMPALWAYARDL
                     FQTPGFGDTIDFVQIKQHYYIVHTDINPTQIVPKGPDLSNWLTPHGREALGGRPFGDG
                     TPPGPTREAERVPAAHSVG"
     gene            complement(539..1021)
                     /locus_tag="VGP27_21855"
     CDS             complement(539..1021)
                     /locus_tag="VGP27_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2771 domain-containing protein"
                     /protein_id="HEV7853977.1"
                     /translation="MKKGLAVLAAVVVLASVATGVLVWRLSRTPPPKHPEISAYTRGQ
                     LVRIGPYQYCAVLNPTDCETPHTDGELTVDPRHPVQLSVPASIASAPWILVRTYEDSD
                     VVSEFRSGSTHAVTIPTTDPHRGRLFGFAVQLPTIVRDEQGNEFPWAHAEWSVKTVWP
                     "
     gene            complement(1018..>2124)
                     /locus_tag="VGP27_21860"
     CDS             complement(1018..>2124)
                     /locus_tag="VGP27_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7853978.1"
                     /translation="ALDRLQHGRRLALATSFALRTALVVVLIANYDGATGSFPSWVLY
                     PCALGMMVLSKSFSVLRSAVTPRVLPPTIDLVRVNSRLTVFGLLGGTIVGGAVAAGAE
                     YLFNLFHMPGALYVIVALTLAGAALSMRIPKWVEVTEGEVPATLSYHGGDGELRRHPQ
                     RVSDHARQPLGRNIITALWGNCTIKVMVGFLFLYPAFVAKSHDKSGWEQLLILGLIGA
                     AAAIGNFGGNYASARLQLGHPAQLVVRSSTAVTLAALATALTGKLMVAALAALVTSAA
                     SAIAKASLDASLQDDLPEESRASAFGRSESLLQLAWVAGGATGVLVYTQLHVGFTVIS
                     TMLILGLAQTLVSYRGDSLIPGLGGNRPVLAAQE"
BASE COUNT          390 a          723 c          695 g          316 t
ORIGIN      
        1 tgcgcgacga gatcgacgag gtcgcccagc gcgtctacac cgaggtgaac aacggcgtgt
       61 accggtgcgg cttcgccggc tcgcaggagg catacgacaa ggcctacgac cggttgttca
      121 ccgcgctgga ttggctcaac gaacggttgg agaaccagcg atatctggtc ggcgacacca
      181 tcaccgaggc agatgtccgg ctgttcacca cgctggcccg cttcgatccc gtttaccacg
      241 gccacttcaa gtgcaaccgg tccaagctcg acgagatgcc tgcgctgtgg gcctatgccc
      301 gcgacctgtt ccagactccg ggcttcggcg acaccatcga cttcgtccag atcaagcagc
      361 actactacat cgtgcacacc gacatcaacc cgacgcagat cgtgcccaag ggccccgacc
      421 tgtccaactg gctgacgccg cacggccggg aagccttggg cggcaggccc ttcggcgacg
      481 gcacgccacc cgggccgaca cgcgaagccg agcgggtacc cgcggcgcac tccgtcggct
      541 aaggccagac cgtcttcacc gaccattcgg cgtgcgccca cgggaactcg ttgccctgct
      601 cgtcgcggac gatcgtcggc agctgcaccg cgaagccgaa caacctgccg cgatgcggat
      661 ccgtcgtcgg gatggtgacg gcatgcgtgc tgcccgaacg gaattcgctg acgacgtcgg
      721 aatcctcgta ggtccgcacc aggatccagg gcgcactggc gatcgaggcc ggcacggaca
      781 attgcaccgg gtgccgcgga tcgacggtca gttcgccgtc ggtgtggggt gtttcgcaat
      841 ccgtggggtt gagcaccgcg cagtactgat acggaccgat ccgcaccaac tgcccgcgcg
      901 tgtaggcgct gatctccggg tgcttgggcg gcggggtgcg gctcaaccgc cagaccagca
      961 caccggtcgc aacagacgcc agcaccacga cggccgccag taccgccaga ccctttttca
     1021 ctcttgggcc gccagcaccg ggcggttgcc gccgagcccg gggatcagcg aatcgccacg
     1081 gtagctcacc agcgtctgcg cgaggccgag gatcagcatg gtcgagatca ccgtaaagcc
     1141 aacgtgcagc tgggtataca ccagcacacc ggtggcgcca ccggccaccc atgcgagttg
     1201 cagcagcgat tcggagcgcc cgaaggccga ggcgcgcgac tcctccggta gatcgtcctg
     1261 cagcgacgcg tccagcgagg ccttggcgat cgcgctggcg gcagaggtca ccaatgcggc
     1321 cagtgcggcg accatcagct tcccggtcag cgcggtggcc aatgcggcca acgtgaccgc
     1381 agtcgagctg cgtaccacca actgcgccgg atgcccgagc tgcagccgcg cgcttgcgta
     1441 gttaccgccg aagttgccga tcgccgcggc cgcaccgatg aggccgagaa tcaacaactg
     1501 ctcccagccg ctcttgtcgt gcgacttggc cacgaacgcg ggatagagaa acaggaagcc
     1561 caccatcacc ttgatggtgc agttgcccca cagtgcagtg atgatgttgc ggcccaacgg
     1621 ttgtcgtgcg tggtcggaga cgcgttgcgg gtggcgacgc agttctccgt caccgccgtg
     1681 gtagctcagt gtggcgggca cctcgccctc ggtgacctcc acccatttcg gtatgcgcat
     1741 cgacagcgcc gcgccggcca gcgtgagggc gacgatgacg tagagggcgc cgggcatgtg
     1801 gaacaggttg aacaggtact cggcccctgc ggccacggcg ccgccgacga tcgtgccgcc
     1861 gagcaggccg aagacggtca gccgcgagtt gactcgcacc aggtcgatgg tcggcggcag
     1921 cacgcggggc gtcacggcgc tgcgcaacac gctgaatgac ttggacagca ccatcattcc
     1981 caacgcacat gggtacagca cccacgacgg gaagctgccc gtcgcaccgt cgtagttcgc
     2041 gatcaacacc acgaccagag cggtgcgcag cgcgaacgat gtggccaggg cgagtcggcg
     2101 gccgtgctgg agacggtcca gcgc
//
LOCUS       DASYLX010000934         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_825650,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000934 DASYLX010000000
VERSION     DASYLX010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825650"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2751)
                     /gene="infB"
                     /locus_tag="VGP27_21865"
     CDS             complement(7..2751)
                     /gene="infB"
                     /locus_tag="VGP27_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV7853979.1"
                     /translation="MAGKARVHELAKELGVTSKEVLARLSEQGEFVKSASSTVEAPVA
                     RRLRESLGGAKSAADKVKADGNGAPAKVAAASAPAPAKAPVAKPAAPAAPAAPAQPAP
                     APAPAEPAAPAAEAMPAASASAASAPAPARPGPTPGPRPATPGPKPAPRTPRVGNNPF
                     SSAQPVERAIPRPQAPRPGAPRPGGGPRPGASPGNMPPRPSGATGRPARPGGGARPAP
                     GGRGPGGGGGRPGGPPGGGGNYRTGGPGGGGGGGGAPAGGGYRGRPGGGGGRPGQRGG
                     AAGAFGRPGGAPKRGRKSKRAKRAEYENMQAPVVGGVRLPHGNGETIRLARGASLSDF
                     AEKIDANPASLVQALFNLGEMVTATQSVGDETLELLGSEMNYVVQVVSPEDEDRELLE
                     SFDLSYGEDEGDESDLASRPPVVTVMGHVDHGKTRLLDTIRQANVREEEAGGITQHIG
                     AYQVAVDFDGGQRLITFIDTPGHEAFTAMRARGAKATDIAILVVAADDGVMPQTVEAI
                     NHAQAADVPIVVAVNKIDKEGADPSKIRGQLTEYGLVPEEFGGDTMFVDISAKQGTNI
                     EALEEAVLLTADAALDLRANPDMEAQGVAIEAHLDRGRGPVATVLIQRGTLRVGDSVV
                     AGDAYGRVRRMVDEHGEDIEAALPSRPVQVIGFTSVPGAGDNLLVVDEDRTARQIADR
                     RSARKRNALAARTRKRISLDDLDAALRETSQLNLILKGDNAGTVEALEQALLGIEIDD
                     EVELRVIDRGVGGVTETNVNLASASDAVIIGFNVRAEGKATELANRDGVDIRYYSVIY
                     QAIDEIESALKGMLKPIYEEKELGRAEIRAVFRSSKVGNIAGCLVTSGVIRRNSKARL
                     LRDSVVVNQQLTVSSLRREKDDVTEVRDGYECGLTLTYSDIKEGDVIETYELVEKERT
                     "
     gene            complement(2832..3191)
                     /locus_tag="VGP27_21870"
     CDS             complement(2832..3191)
                     /locus_tag="VGP27_21870"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV7853980.1"
                     /translation="MIQRETSASPHQSTEGPVRTCVGCRKRELAAGLLRVVARNSGNG
                     VYAVTVDRGGNLPGRGAWLHPDQQCLHAAIRRRAFARALRITGSPETSAVLEHFESLE
                     APRLPATEQAAKNMSTP"
     gene            complement(3240..4283)
                     /gene="nusA"
                     /locus_tag="VGP27_21875"
     CDS             complement(3240..4283)
                     /gene="nusA"
                     /locus_tag="VGP27_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV7853981.1"
                     /translation="MNIDMAALHAIESDKGISVDVVVDTIKSALLTAYRHTEGHEADA
                     RIEIDRKTGVVQVLARETDEDGNVLNEWDDTPEGFGRIAATTARQVILQRLRDAENEK
                     NYGEFSTREGEIVGGVIQRDSRANARGLVVVRMGSETKGSEGVIPSAEQVPGESYEHG
                     DRLRCYVVGVSRGAREPLITLSRTHPNLVRKLFSLEVPEIADGSVEIVAVAREAGHRS
                     KIAVTSRAPGLNAKGACIGPMGQRVRNVMSELSGEKIDIIDYDEDPAKFVANALSPAK
                     VVSVQVIDEAARAARVVVPDFQLSLAIGKEGQNARLAARLTGWRIDIRSDAAAAADTP
                     SKPDATHGAVHDR"
     gene            complement(4280..4837)
                     /gene="rimP"
                     /locus_tag="VGP27_21880"
     CDS             complement(4280..4837)
                     /gene="rimP"
                     /locus_tag="VGP27_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV7853982.1"
                     /translation="MASDPQLRSARLPSQQQVIELLDGEFARAGYEIEDVVINAGARP
                     PRITVIADGDEGLDLDSIARLSRSASELLDSADASKSDSPYVLEVTSPGVDRPLTEEK
                     HYRRARGRKVELTLSDGSQLMGRLGETRDGAVDLVVREGAGENYSVRELPLSGIAKGV
                     VQVEFSAPNQRELELAGQTGKGAGT"
     gene            4959..5450
                     /locus_tag="VGP27_21885"
     CDS             4959..5450
                     /locus_tag="VGP27_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853983.1"
                     /translation="MASVPSASPVVSRRRMLLGTTALAALGVTAAACGKPPPPPELGD
                     LTTQLDMARADSQLASDAAATAEPPLRPALAAVSADRSKHAEALTDEIARLTGEAATS
                     SSTTTTTAAQAKAPTTKDVVDSLRTSADSAGRLAAQMSGYRAGLLGSIAASCTAAYTV
                     ALA"
     gene            5447..>5568
                     /locus_tag="VGP27_21890"
     CDS             5447..>5568
                     /locus_tag="VGP27_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4439 domain-containing protein"
                     /protein_id="HEV7853984.1"
                     /translation="MTSAEPTGPARPPDPDAAALFDAVTTEHAVIYGYGLVSAHS"
BASE COUNT          769 a         1951 c         1885 g          963 t
ORIGIN      
        1 gaccatctat gtgcgctcct tttcgacgag ctcgtacgtt tcgatgacgt cgccttcctt
       61 gatgtcggaa taggtcagcg tcaaaccgca ctcgtagccg tcgcgcacct cggtgacgtc
      121 gtccttctcc cggcgcagtg aggacacggt gagttgctga ttgaccacca cgctgtcgcg
      181 cagcaggcgt gccttcgagt tgcggcggat gaccccggag gtgaccaggc agccggcgat
      241 gttgccgacc ttggacgacc ggaagaccgc acggatctcc gcccggccca gctccttctc
      301 ctcgtagatc ggcttgagca tgcccttgag cgcgctctcg atctcgtcga tggcctggta
      361 gatcaccgag tagtagcgga tgtcgacgcc atcgcggttg gccagctcgg tggccttccc
      421 ctcggcgcga acgttgaagc cgatgatcac cgcatccgat gccgacgcca gattgacgtt
      481 ggtttcggtg acgccgccga cgccgcggtc gatgacgcgc agttccacct cgtcgtcgat
      541 ctcgatgccg agcagggcct gctccagcgc ctcgaccgtg cccgcgttgt cgcccttgag
      601 gatcaggttc agctgactgg tttccctcag tgcggcgtcc agatcgtcga ggctgatgcg
      661 cttgcgcgta cgcgccgcca gggcgttgcg cttgcgggcg ctgcgacggt cggcgatctg
      721 ccgcgcggtg cggtcctcgt cgacgacgag gaggttgtca ccggcgcccg gcaccgacgt
      781 gaagccgatg acctgcaccg gccgtgacgg cagcgccgcc tcgatgtcct cgccgtgttc
      841 gtcgaccatg cgacggaccc ggccgtaggc gtcgcccgcc accaccgagt cgccgacccg
      901 cagcgtgccg cgctggatca gcacggtggc caccggaccg cgaccgcggt cgaggtgcgc
      961 ctcgatcgcc acaccctggg cctccatgtc cgggttggcc cgcaggtcga gcgccgcgtc
     1021 ggcggtcaac agcaccgcct cttccagcgc ctcgatgttg gtgccctgct tggccgagat
     1081 gtcgacgaac atggtgtcgc cgccgaactc ctcgggcacc agaccgtatt cggtcagctg
     1141 gccgcggatc ttcgacggat cggcgccctc cttgtcgatc ttgttgaccg ccaccacgat
     1201 cggcacatcg gccgcctgcg cgtggttgat ggcctccacc gtctgcggca tgacgccgtc
     1261 atcggccgcg accaccagga tcgcgatgtc ggtggccttg gcgccgcggg cacgcatggc
     1321 ggtgaacgcc tcgtggcccg gggtgtcgat gaaggtgatc agccgctggc cgccatcgaa
     1381 atcgacggcg acctggtagg caccgatgtg ctgggtgatg ccgccggctt cctcctcgcg
     1441 gacgttggcc tggcggatcg tgtccagcag tcgggtcttg ccgtggtcga cgtgacccat
     1501 cacggtgacc accggcggac gacttgccag gtcggactcg tcgccctcgt cctcgccgta
     1561 gctcagatcg aaggactcga gcagttcgcg gtcctcgtcc tctggcgaga ccacctgaac
     1621 gacgtagttc atctcgctgc ccagcagttc gagggtttcg tcgccgacgg actgggtggc
     1681 ggtcaccatc tcaccgaggt tgaacagcgc ctgcaccagc gaggccgggt tggcgtcgat
     1741 cttctcggcg aagtcgctca gcgatgcgcc gcgggccagc cggatcgtct ctccgttgcc
     1801 atgcggcaac cgaacgccgc cgacgacggg cgcctgcatg ttctcgtatt cggcgcgttt
     1861 cgcccgcttc gacttgcggc cgcgcttggg cgcaccaccg ggacgaccga acgcacctgc
     1921 ggcaccgcca cgctggccgg gacggccgcc accaccaccg ggacggccgc gatagccgcc
     1981 tccagcgggt gcaccgccgc caccgccacc accaccgggg ccgccggtgc ggtagttacc
     2041 gccgccgccg ggggggccac cgggacgtcc gccgccacca ccggggccgc ggccgccggg
     2101 agcgggccgc gcgccgccgc ccgggcgtgc cggacggcct gttgcgccac tgggacgggg
     2161 cggcatgttg cctggcgacg cgccgggacg tggaccgccg ccgggcctcg gagccccggg
     2221 tcgcggtgct tgcggccgcg gaatcgcccg ctcgaccggc tgcgccgagg agaacgggtt
     2281 gtttccgacg cgcggagtgc gcggcgcggg cttgggtccc ggcgttgcgg gccggggacc
     2341 cggtgtcggg cccgggcgtg ccggtgcggg agccgatgcg gccgacgccg acgcagcagg
     2401 cattgcctcc gcggcgggtg ccgcgggctc tgcgggtgcg ggtgccggcg ccggttgcgc
     2461 cggggctgcg ggcgctgctg gagccgccgg cttggcgacc ggcgccttgg cgggcgcggg
     2521 tgccgacgcg gccgccacct tggcgggcgc accgttgccg tcggccttga ccttgtccgc
     2581 cgcggacttt gctccgccaa gggattcgcg cagccgacgc gcgacggggg cctccacggt
     2641 ggaggatgcg gatttaacga attcgccctg ttcactcagt cgggcgagta cttccttgct
     2701 ggttacaccg agttccttag ccaactcgtg tacgcgggcc ttgcctgcca cttctctcct
     2761 gtcttagagg cgacagcggt ggtagggccg cgcctcgggt ttagctatga cgcatggtca
     2821 tcgtctggac ttcacggtgt gctcatgttc ttcgctgcct gttctgtagc cgggagccga
     2881 ggcgcctcga gtgattcgaa gtgctccagc accgcggatg tttccggtga gccggtgatg
     2941 cgcaacgctc gagcgaatgc tcgccgccgg attgctgcgt gcaaacactg ctggtcaggg
     3001 tgcaaccaag cgccccgccc aggcaggtta cctcctcggt caacggttac ggcgtaaacg
     3061 ccattcccgc tgttgcgagc aaccacccga agcagtccgg cggccagctc tcgctttcgg
     3121 cagccgacgc atgtccgtac gggtccttcc gtactttgat gcggcgaagc cgaagtctcg
     3181 cgctggatca cgcctgagtc taccgtcccg ccccccgcga aacgaaacgg ccgaatccgc
     3241 tatctgtcgt gcacagcgcc gtgtgtcgcg tccggtttcg acggggtgtc cgcagcggcg
     3301 gcggcatcgc ttcggatatc gatgcgccag ccggtcaacc gggccgcgag ccgggcgttc
     3361 tggccctcct tgccgatcgc cagcgacaac tggaagtcgg gtaccaccac tcgggccgcg
     3421 cgggcggcct cgtcgatcac ctgcacggac accaccttgg cgggcgaaag agcgttggcg
     3481 acgaacttgg ccgggtcctc gtcgtagtcg atgatgtcga tcttctcccc ggagagctcg
     3541 ctcatcacgt tgcgcacccg ctggcccatc ggccctatgc aggcgccctt ggcgttgagc
     3601 cccggcgccc gtgaggtcac cgcgatcttg gaccggtggc ctgcctcgcg ggccaccgcg
     3661 acgatctcga cggagccgtc ggcgatctcg ggcacctcca gcgagaacag cttgcggacg
     3721 aggttcgggt gcgtgcgcga cagcgtgatc aacggttcgc gggcaccgcg ggagacgccg
     3781 acgacgtagc aacgcagccg gtcgccgtgt tcgtagctct cgccggggac ctgctcggcg
     3841 gatggaatga cgccctcgga acccttcgtc tcgcttccca tccggacgac gacgaggccg
     3901 cgggcgttgg cgcggctgtc gcgctggatg acgccgccga cgatctcgcc ctcgcgagtg
     3961 gagaactcgc cgtagttctt ctcgttctcg gcgtcgcgca gccgctgcag gatgacctgg
     4021 cgcgcggtgg tggccgcaat gcggccgaat ccctctgggg tgtcgtccca ttcgttgagg
     4081 acgttgccgt cttcgtcggt ttcgcgcgcc agcacctgca cgacgccggt cttgcggtcg
     4141 atctcgatcc gagcgtcggc ttcgtggccc tcggtatggc gatatgcggt aagcaacgcg
     4201 gacttgatgg tgtcgacgac gacgtcgacc gagatgccct tgtcggactc gatcgcgtgc
     4261 aatgcggcca tgtcgatatt catgttccgg cccccttccc cgtttgaccg gccagctcga
     4321 gctcccgctg atttggagct gaaaactcca cttggacaac gcctttggcg atgccactga
     4381 gtggcagttc gcgcacggaa tagttctccc ccgctccttc gcgcaccacc agatcgactg
     4441 cgccgtcccg cgtttcgccg agcctgccca tcagttgcga cccgtcggac agcgtcagct
     4501 cgaccttgcg gccgcgcgcc cgccgataat gcttctcctc ggtgagcggg cggtccacgc
     4561 ccggcgacgt cacctcgagg acatagggcg aatcggattt cgacgcgtcg gcggagtcga
     4621 gtagttcgga tgcggaccgg gacagccggg cgatcgagtc gaggtcgagg ccttcgtcgc
     4681 cgtcggctat caccgtgatc cgtggcgggc gagcgccggc gttgatgacg acgtcctcga
     4741 tttcgtaacc tgcgcgcgcg aactcgccgt cgagtagctc gatcacctgc tgctgcgagg
     4801 gcaatctcgc agaccgcagc tgcggatccg atgccacggc gagctcctca tgttgaattg
     4861 tgccgacgtg gccaacgata cgccagtgct cagcaggcga accggattcc cgcagccctg
     4921 tccagcgaat acacagagcc gttttcggca atggcaggat ggcatccgtg ccgagtgcct
     4981 cacccgtcgt cagcaggcgg cgcatgctgc ttgggacgac ggcgctggcg gcgctgggcg
     5041 tcaccgccgc ggcatgcggc aagccaccgc cgccgccgga gctcggcgat ctgacgacgc
     5101 aattggatat ggcccgcgcc gacagccagc tggccagcga cgccgccgcg accgccgagc
     5161 cgccgctgcg gcccgcgctg gccgctgtct cggccgatcg gtcgaagcac gccgaggcgc
     5221 tgaccgatga gatcgcgcgt ctgaccggcg aagccgccac gtcgtcgtcg accacgacga
     5281 ccaccgccgc gcaggcgaag gcgcccacga caaaggacgt cgtggactcg ctgcgcacgt
     5341 ccgccgacag cgcgggccgg ctggcggccc agatgtccgg ttaccgggcc gggctgctcg
     5401 gctcgatcgc cgcatcgtgt accgcggcct acacggtggc gctcgcgtga cctctgccga
     5461 accgaccggt cctgccaggc cacctgaccc cgatgcggcc gcgctcttcg acgccgtgac
     5521 caccgagcac gccgtgatct acggctacgg cctggtgtcg gcgcactc
//
LOCUS       DASYLX010000935         6305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3677021,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000935 DASYLX010000000
VERSION     DASYLX010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677021"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VGP27_21895"
     CDS             <1..1459
                     /locus_tag="VGP27_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV7853985.1"
                     /translation="TATPDRPTPGQRPHVLREAAVSLLDLSRWQFAITVLFHMTFPSI
                     TVGLSILLCVLYGLFWRTGNPVYRQLFRFWRRIFAVGFALGVVAGIVITFEMGLNWGS
                     YAAETGPIIAPILGMEVVMAFAVEAGFIGVVLYGDGRVRQGTMFAATVMVSIGTILSS
                     TWIMAANSWMQTPTGFVMRNGRFEPTNWIDVIFNPSFGWRYPHMLVGVLVSASFFVAG
                     IGAYYLVKGRALRFARRSVSVALGVAAILLPVQVFLGDGVATHLFPYQPSKAEAAEGD
                     WTSGNTGWIIFAIPDQQAQRNIAQVSIPCLASAFYGDLSCTTGMPGLDLTPKADQPMM
                     APVFWGFRVMFYASLLMFGTVFYATILRFRRKLWTSPRFHRFLLWSTPMGIIAVLSGW
                     ILAETGRQPWVVFGHLRTSAAVSHLAPDEILFSVLGFSLLYLVMLAGYLAYIVRTMRI
                     GPEGDHPDLIGRSPATPVPVPAATGVIAPQPASGR"
     gene            1464..2468
                     /locus_tag="VGP27_21900"
     CDS             1464..2468
                     /locus_tag="VGP27_21900"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV7853986.1"
                     /translation="MATAWFVLMVLCLLMYVVLDGYDLGIGVATLFERDRQRRYDMLE
                     LVAPAWDGNETWLVLLVVSLWAGFPLAFGTILPHAYLPLIVVLLSLIVRGISVEMASQ
                     EYPAPRWERAFGVGSLVASVAQGVVLGTLTSKLTVVDGAFSGSPFGSIGWFSVLTAAA
                     VTCGYLALGYAYTKQKATGELRAIAGRRGTVSTVLASVLAVACLAALNGTAAPLNLHG
                     PGRAIAFTGLLLFAAASVVMALVTLRPAARDDSVPLAGLATATMALVIAVVVARYPVL
                     APPTLTIADTAAPHTTMVFLAVGIGLNVPLVLFYNWFAHHTFRGKLTSAPDGRDMPHA
                     "
     gene            2482..2727
                     /locus_tag="VGP27_21905"
     CDS             2482..2727
                     /locus_tag="VGP27_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7853987.1"
                     /translation="MTTEPDPARRPPRTAHRFFTRALLTVVGVIGGVLSYCICVGTFA
                     GYPQYHTDVLTIIGMLLIALIGGVAWIIDERRIDADG"
     gene            2714..4435
                     /locus_tag="VGP27_21910"
     CDS             2714..4435
                     /locus_tag="VGP27_21910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853988.1"
                     /translation="MPTAEQSGSASLSGQPRGWLASAAAPGRTLLRLAATCQALETVF
                     TVVQWAALAWAAQDVFARRTQPTWPTLGALFVGGLLAAGAAWSAARFQAAGGQRIAHA
                     IRQRLVAGLLPSGQRRGEPDTATAALATVELTDDVADYHAQALPQRLSAPASMAVVFL
                     ATAAVQWPAAGILLVSSLFIPLNMRLAGLFAQEGADERVTATTRLAAVVLDSFRGMRT
                     LHSIGAVARRRDELADAAADLDATTMAIVRRAFLSGSVMDVVITFSIAANATYIGLSL
                     LGYVRLDAAPTMTLFGGLFALLLCPMYFQPLRAMAAAYHAQERALSAVPTIIALLTEV
                     HAVPQIGERCAPRPAGSIAVVLDNITFRFPHSNDPVLHGVDLTVRPGRWTAIVGPSGV
                     GKTTLLSLIAGVRQPSTGTMRWVTATGASPPHLGGCAWIGQQTVLLPGTISDNIRIGR
                     PAASLADVEHAVAAAGLADLIARLPEGLDTPLSESSPGLSTGEARRIAIARAFLSDAE
                     LWVLDEPTAHLDLHAEAQVIDALHNATQGRTVIVATHSAALARSADAVLSLADGTIHA
                     TREAIPA"
     gene            4432..6036
                     /locus_tag="VGP27_21915"
     CDS             4432..6036
                     /locus_tag="VGP27_21915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7853989.1"
                     /translation="MNPNAQRRDRSQHSRSALVSVAGLALAVLAESCAVGLLGLSGWF
                     IATSAVAGASAYSAFSYLAPSGGVRAFALGRIATNYAHRVVLHSAALRRISAARLKFY
                     DRTAAEPSTHGTWSGQSLDRVMADADTKGMALIQATGPMIVAAAMTAGGCLAVVLAGY
                     PLVAVVLAAAAAVCAALAIVTARRMDDGSRTRSALRTELVTAVGAWTEMASLGAADQL
                     AQRTLRRLAIFEHHRLHHAMTSARTLGAARAVTAAALLITVTLAARSGATVSTLVFLA
                     LLTAGVIVNAERLVAATQAWTLAHQADERLTSVGSDEIRRPSRPPILRATYDRSGLTV
                     SDYWLPDTPTRNARPIEFAVAAGQTLIVTGSSASGKTTLLGAIATTLRQPAIQPAPGV
                     VTVVLADDYLFTGTASTNVRLANPTATDDDIADLLTSMLLDRSGLEPNTKIGLNGRSL
                     SGGEQRRLHIARALTTQPDVLLIDEPTTGLDTSTGTHVLMTIRRRLPLAVLVLAMHEL
                     PANPDALGPAWSTLSLDRRSLAPSYR"
BASE COUNT          957 a         2264 c         1958 g         1126 t
ORIGIN      
        1 cacagcaacc ccggaccgcc caacgccggg tcagaggccg cacgtattgc gggaggcagc
       61 ggtgagcctg ctcgatctct cacgatggca gttcgccatc acggtgttat tccatatgac
      121 gttcccgtcg atcaccgtcg ggttgtcgat cttgttgtgc gtcctgtacg ggctgttctg
      181 gaggacgggc aatcccgtat atcggcagct gtttcgtttc tggcggcgga tcttcgcggt
      241 cgggttcgcg ctcggcgtgg tcgccggcat cgtgatcacc ttcgaaatgg ggctcaactg
      301 gggtagctac gccgcggaaa ctgggccgat catcgccccc atcctgggga tggaggtcgt
      361 catggcgttc gccgtggagg cgggcttcat cggcgtcgtg ctctacggtg acggccgggt
      421 caggcagggc acgatgttcg ctgccacggt gatggtctcg attggcacga tcctgtcctc
      481 gacctggatc atggccgcca actcgtggat gcagacgccg acgggattcg tcatgcgtaa
      541 cgggcggttt gagccgacga actggataga cgtcatcttc aacccatcgt tcgggtggcg
      601 atacccgcac atgctggtcg gggtgttggt ctcggccagc ttcttcgtcg ccggcatcgg
      661 ggcctactac ctggtcaagg gaagagcgct gcggttcgct cggcgctcgg tgtccgtcgc
      721 gctgggcgtc gcggcgatcc tgctgccggt ccaggtgttt ctgggtgacg gggtcgccac
      781 tcacctgttt ccgtaccagc cctccaaggc tgaggccgcc gagggcgact ggactagtgg
      841 aaacactggc tggatcatct tcgcgatccc cgaccagcag gcgcagcgca acatcgcgca
      901 ggtctccatc ccctgcttgg ctagtgcgtt ctacggagat ctgagctgca cgaccgggat
      961 gcccgggctc gacctgacac cgaaagccga ccagccgatg atggcgccgg tcttctgggg
     1021 attccgcgtc atgttctatg cgtcgctgct gatgttcggc acggtcttct acgcgacgat
     1081 cctgcgtttc cggcgcaagc tgtggacctc gccacggttt caccgattcc tgctgtggtc
     1141 gacccccatg gggatcatcg ccgttctgag tggatggatc ctcgcggaga ccggtcgcca
     1201 gccatgggtg gtgttcggcc atctccgaac ctccgccgcg gtttcacatc tggcgccgga
     1261 cgagatcctg ttctcggtct tgggcttctc gctgctttac ctggtgatgc tggctggcta
     1321 cctcgcctac atcgtgcgca cgatgcggat cgggcccgag ggcgatcacc ccgacctgat
     1381 cggccgatcg ccagcaacgc ccgttcccgt cccggcagcg accggagtca tcgcgcccca
     1441 gccggcgagc gggaggtgag tcgatggcca ccgcttggtt tgtcctcatg gtgctgtgcc
     1501 tgttgatgta tgtcgtgctc gacggctatg acctgggcat cggggtcgcc accttgtttg
     1561 agcgtgaccg acaacgtcga tacgacatgc tcgaattggt cgccccagcc tgggacggga
     1621 acgagacgtg gctggtcttg ctagtcgtaa gcctctgggc cggttttccc ctggcgtttg
     1681 gcaccatcct tccgcacgcc taccttccat tgatcgtcgt gttgctctcg ttgatcgtcc
     1741 gcgggatcag cgtggagatg gcatcccagg aatatcccgc gcctcgctgg gagcgagcgt
     1801 tcggcgtcgg ctcgctggtg gcgtctgtgg cgcagggcgt cgtcctcggc accctgactt
     1861 cgaagctgac ggtggtggac ggcgcgttct ccggctcacc cttcgggtcc atcggttggt
     1921 tctccgtgct caccgcggcg gctgtcacgt gcggctacct ggcgctgggc tacgcctata
     1981 ccaagcagaa ggcgaccggc gagctgcggg caatcgccgg acgtcgcggc acggtcagca
     2041 ccgtcctggc gtcggtgctc gcggtggcct gtctcgccgc cctcaacggc accgccgcgc
     2101 ccctgaacct gcatggcccc gggcgggcga ttgccttcac cggactgctg ctgttcgcgg
     2161 cggccagcgt ggtgatggcc ctggtgacgc tgcggcccgc cgctcgggac gactctgtcc
     2221 ccctcgccgg gctggcgacc gccaccatgg cgctggtcat cgccgtggtc gtggctcgct
     2281 accccgtgct ggctccgccc acgctcacga tcgccgacac ggcggcgccg cacaccacga
     2341 tggtgtttct cgccgtcggg atcggactca acgtgccgtt ggtcctcttc tacaactggt
     2401 tcgcccacca caccttccgg ggcaagctca ccagcgcccc tgacggccgc gacatgcccc
     2461 acgcttagcc aggagccatc catgaccacc gaacccgatc ccgcgcggcg gccgccgcgg
     2521 acggcccacc gattcttcac ccgcgcgctc ttgaccgtcg tcggtgtcat cggcggcgtg
     2581 ctgagttact gcatctgtgt ggggacgttc gcgggctatc cccagtacca caccgacgtg
     2641 ctcaccatca tcggcatgct tctcatcgcc ctgatcggcg gcgttgcctg gatcatcgac
     2701 gaacgccgaa tcgatgccga cggctgagca gagtggtagc gcaagcctgt ccgggcagcc
     2761 gcgcggctgg cttgcctccg ccgccgcgcc tggccgcacc ttgctgcggc tcgctgccac
     2821 gtgccaggcg ctggagacgg tgttcaccgt cgtccaatgg gcggcgctgg cctgggccgc
     2881 gcaggacgta tttgcccggc ggacacaacc cacctggccc acgctgggtg ccctgttcgt
     2941 cggcggtctg cttgcggccg gggcggcatg gagcgcagca aggttccagg ccgcgggcgg
     3001 ccaacggatc gcccacgcca tccggcaacg tctcgtggcc gggctactgc cctcgggaca
     3061 gcggcgcggt gaaccagata ccgccacggc agccctggcg accgtcgagt tgaccgacga
     3121 tgtcgccgac taccacgccc aagcccttcc tcaacgcctg tccgctccag cgtcgatggc
     3181 tgtcgtcttc ctcgccaccg cggcggtgca atggccggcc gcggggatcc tgcttgtctc
     3241 aagcctgttc atcccgctga acatgcgcct cgccggcctg ttcgcccagg aaggcgcgga
     3301 cgagcgggtg acagccacca cgagactcgc agctgttgtc ctggacagct ttcgcggcat
     3361 gcgcacgctg cacagcatcg gagctgtggc gcgccgacgc gatgagctcg ccgatgcggc
     3421 cgccgacctg gacgccacca ccatggcgat cgtgcggcga gcgttcctct ccggttcggt
     3481 gatggacgtc gtcatcacct tctcgattgc agccaacgcg acgtatatcg gactgtctct
     3541 gctcggctac gtccggctgg acgccgcacc cacgatgacg ctcttcggcg gtctgttcgc
     3601 gctgctgctg tgcccgatgt acttccagcc gcttcgcgcg atggccgccg cctatcacgc
     3661 ccaggagcgg gcgctgtcgg cggtgccgac gatcatcgcg ttgctcaccg aagtccacgc
     3721 ggtgccgcag attggcgaac gctgcgcgcc gcgacccgct ggatccatcg cggtcgtcct
     3781 ggacaacatc acgttccgct ttccccactc gaacgatccg gtcctgcacg gggtcgacct
     3841 gacggtccgc cccggccgct ggacggcgat cgtcgggcca tccggcgtcg gcaagacgac
     3901 cctgctttcg ctgatcgccg gcgtgcgcca accgtccacc gggaccatgc gctgggtcac
     3961 cgccaccggc gcctcgccgc cacacctggg cggttgtgcc tggatcggcc aacaaacggt
     4021 gcttctcccc gggactatca gcgacaacat ccgcatcgga cgacccgctg cgagccttgc
     4081 cgatgtagag catgcggttg ccgcggcggg gctggccgac ctcatcgcac ggctgcccga
     4141 aggactggac acaccactga gtgagagcag cccgggtctg tcgaccggcg aggcccgtcg
     4201 catcgccatc gctcgtgcct tcctcagcga cgccgagctc tgggttctcg acgaaccgac
     4261 cgctcacctc gatctacacg ccgaggcgca agtgatcgat gcgctacaca acgccacgca
     4321 aggcaggacg gtcatcgtag ccacgcactc ggcggcgctg gcccgctccg ctgacgccgt
     4381 gctcagcctc gccgacggga ctatccacgc cacccgggag gcgatcccgg catgaacccg
     4441 aacgcgcaac gtcgcgaccg gagccaacac agccgtagcg ccctggtgtc cgtcgcagga
     4501 ctggccctcg ccgtcctcgc cgagagctgc gcggtcgggc ttctcgggct ctcgggatgg
     4561 ttcatcgcca cctcggccgt ggcaggagcc agcgcgtaca gcgcgttctc ctacctggct
     4621 cccagcggcg gagtgcgcgc cttcgccctg ggcaggatcg cgacaaacta cgcccaccga
     4681 gttgtgttgc actccgcggc actgcgccgg atcagcgccg ctcgactcaa gttctacgac
     4741 cgcaccgctg cagaacccag tactcacgga acctggtctg ggcagtctct ggatcgggtc
     4801 atggccgacg cggacaccaa aggcatggcg ctcatccaag ccaccggacc catgatcgtg
     4861 gccgcggcga tgaccgcggg cggctgcctt gcggtcgtgc tcgcgggtta ccccctcgtg
     4921 gcggtggtgc tggccgctgc cgctgccgtc tgcgccgcgc tcgcgatcgt cacggcgcgc
     4981 cggatggacg acggaagccg cacgcgcagc gcgctgcgaa ccgagctcgt caccgcggtc
     5041 ggggcctgga ccgagatggc atcactaggc gctgccgacc aactcgcgca gcgcacgctg
     5101 cggcgtcttg ccatctttga gcatcatcga ctccaccacg cgatgacgag cgcacgtacc
     5161 ctgggcgccg cccgcgccgt gaccgccgcc gcgttgctga tcaccgtcac actcgcggca
     5221 cgaagcggcg cgaccgtctc aacgctggtc ttcctcgctc tgctcaccgc tggcgtgata
     5281 gtcaacgccg aacgcctcgt cgccgccacc caagcgtgga ccctcgccca tcaggccgac
     5341 gagcgcctca cctcagtcgg cagcgacgag atccgccgac ccagccgccc cccgattctg
     5401 cgggcgacct acgaccgcag cggcctgacc gtgtccgact actggctgcc cgacacccca
     5461 acacgcaacg ctcgtcccat cgaattcgca gtcgccgccg gccagacgct gatcgtgacc
     5521 ggttcatccg caagcggcaa gaccacgctc cttggcgcaa tcgcgacaac actgcgacag
     5581 ccagcaatcc aacccgcgcc gggcgtggtc accgtcgtgc tggcagatga ctacctgttc
     5641 accggaacag cctccaccaa cgtacgtctg gccaacccca cggcaaccga cgacgacatc
     5701 gcagacctgc tcaccagcat gttgctggac cgcagcggac tggaacccaa caccaagatc
     5761 ggcctcaacg gacgcagcct ctcgggcggt gaacagcgcc gcctccacat cgcccgtgcc
     5821 ctcaccaccc aacccgacgt gctgctcatc gacgaaccca cgacgggtct ggacaccagc
     5881 accggcaccc acgtactcat gactatccgc cgtcgactcc cactcgcggt actcgtgctg
     5941 gcaatgcacg aattaccagc aaacccagac gccctaggcc ccgcctggtc cacactgtcg
     6001 ttagaccgaa gatcgctagc gccgtcatac cgctagtcat gccggaccat gcccagcgga
     6061 ctgggagtcc aagtggaccc cgaccggggc gagatcggga tgggcggtat cagtggctcc
     6121 tttgccttcg ctcaccccag gctttcatac tcgtctgcgt tcctcacccg cagcctccac
     6181 gaccaccaac gcgtcacgac cctcatcaac gccctcaacg cctgcctatc ctgaggcctc
     6241 caggtcggca gtatgcgtag ggaccaaggc tggcaggaac acacaggccg catttctgac
     6301 tatcc
//
LOCUS       DASYLX010000936         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2471260,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000936 DASYLX010000000
VERSION     DASYLX010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471260"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGP27_21920"
     CDS             complement(<1..239)
                     /locus_tag="VGP27_21920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7853990.1"
                     /translation="MYRGEPAVKGAPRPTGVPWDIRQAQPRLMELESLGGIGGEVLDI
                     GCGLGDNAIFLASRGHSVTGLDGSPTAIEEARNRAA"
     gene            complement(307..846)
                     /locus_tag="VGP27_21925"
     CDS             complement(307..846)
                     /locus_tag="VGP27_21925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7853991.1"
                     /translation="MTARLVHTADLDNEARDDARRMVIEAFDGGEIDFTDADWDHALG
                     GMHALIWHHGALIGHAAVVQRHLIYRNSALRCGYVEGVAVREDWRGQGLARALMDAVE
                     QVIRGAYPLGALSASEAGRAMYTPRGWLPWRGPTSVLAPDGVRRTPDDDGSIFVLPLA
                     FDMDTSESITCDWRDGDVW"
     gene            complement(933..1823)
                     /locus_tag="VGP27_21930"
     CDS             complement(933..1823)
                     /locus_tag="VGP27_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HEV7853992.1"
                     /translation="MTATLEILRTGPLALIEDLGRKGMAHLGVTRSGAADRGAHKLAN
                     RLVANPNDRATIEVTFGGFSARVRGGDVAVAVTGADTDPAVNGVPFGTNSIHYAHDGE
                     VISLGAPHSGLRSYLAVRGGIDVAPVLGSRSYDVMSSVGPLPLQPGDLLPIGDHTEEF
                     PELEQAPVAAIDEEIVELSVVSGPRDDWFTDPDMLVRTNWQVTNRSDRVGMRLVGMPL
                     EYRYGDRQLPSEGATRGAIQVPPNGFPVILGPDHPVTGGYPVIGVVIDEDIDRIAQVR
                     PGQTVRLHWSRPRRPFQNGS"
     gene            complement(1820..2491)
                     /locus_tag="VGP27_21935"
     CDS             complement(1820..2491)
                     /locus_tag="VGP27_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEV7853993.1"
                     /translation="MTVTTDAMVIGRVTDYGDAAVLLQFDSTAEVLAWADVVRDADLL
                     GVVDIVPASRTVLIKLDGPRYQGVARQQLSKLRLAPESLEQTAAPIDGRADVVIDVVY
                     EGADLGEVADLTGLTPREVVAAHTQTPWRVGFAGFAPGFGYLVGGDERLKVPRRSEPR
                     TKVPIGAVGLAGEFSGVYPRESPGGWQLIGHTSEEQSALWDVERDPPALLTPGQWVQF
                     REASA"
BASE COUNT          391 a          880 c          825 g          400 t
ORIGIN      
        1 gctgcgcggt tgcgggcctc ctcgatcgca gtgggcgagc cgtcgagacc ggtcaccgaa
       61 tgtccgcgcg acgcgagaaa gatcgcgttg tcgccgaggc cgcagccgat gtcgagcacc
      121 tcgccgccga tgccgccgag cgactcgagt tccatcagtc gtggctgcgc ctgccggatg
      181 tcccacggca ctcccgtggg ccggggtgcg cctttcacgg cgggctcgcc gcggtagatc
      241 ctgtcgaacg attctggtgt cggctgaaag ggtttgggcg tcgtcacctc atccaaccta
      301 cgcgcgtcac cacacgtcgc cgtcgcgcca atcgcacgtg atcgactcgc tcgtgtccat
      361 gtcaaaagcc agagggagca cgaagatcga accgtcgtcg tcgggagtgc gccgcactcc
      421 gtcgggcgcc agcaccgagg tcgggccgcg ccacggcagc cagcctctcg gggtgtacat
      481 ggctcgccca gcctccgagg cgctgagcgc cccgagcggg taggcgccgc ggatcacctg
      541 ttcgactgcg tccatcagcg cgcgggccaa cccctggccg cgccagtcct cgcgaaccgc
      601 gacgccttcg acatatccgc atcgaagagc cgaattccgg tagatcaggt ggcgctgcac
      661 cacggcggcg tggccgatca gtgcaccgtg atgccagatc agcgcgtgca tgccgccgag
      721 ggcgtggtcc cagtcggcgt cggtgaagtc gatctcgccg ccgtcgaagg cttcgatgac
      781 catgcgacgg gcgtcgtcgc gggcctcgtt gtcgagatct gcggtgtgga ccaggcgcgc
      841 tgtcattacc ccgcgcacgc gaggagcggc gtgactcccg cgcacgtgag gagcggcgtg
      901 actgccagta ggcacaccac gtgtctactc gctcaggaac cgttctggaa gggtcggcgc
      961 ggccgcgacc agtgcagccg gaccgtctgc cccggccgca cctgcgcgat ccggtcgatg
     1021 tcctcgtcga tcacgacgcc gatcaccgga tagccgccgg tgaccgggtg atcgggcccg
     1081 aggatcacgg gaaaaccgtt cggcggcacc tgaatcgcgc cgcgggtggc accctcactg
     1141 gggagttggc ggtcgccgta ccgatactcc agcggcatgc cgaccagtcg catgccgacc
     1201 cggtcgctgc ggttggtgac ctgccagttc gtccggacca gcatgtcggg atcggtgaac
     1261 cagtcgtcgc gagggcccga caccaccgac aactcgacga tctcctcgtc gatcgcggcc
     1321 accggcgcct gctccagttc ggggaactcc tcggtgtggt cgccgatcgg cagcagatcg
     1381 ccgggttgca gcggcagcgg gccgacggac gacatcacgt cgtaactgcg ggagccgagc
     1441 acgggtgcga catcgatgcc tccgcggacc gcgagatagg accgcagccc ggagtgcgga
     1501 gcgccgagcg aaatcacctc gccgtcgtgc gcatagtgaa tactgttggt gccgaacgga
     1561 actccgttga ctgccggatc ggtgtcggca cccgtcaccg caacggccac gtcccctccg
     1621 cggacccgtg cgctgaagcc gccgaatgtc acctcgatgg tggcgcggtc attcgggttg
     1681 gcgaccaatc ggttggccag cttgtgcgca ccgcggtctg cggcgcccga tcgcgtcaca
     1741 ccgaggtgcg ccataccctt gcggccgagg tcctcgatca acgccagcgg accggttcgc
     1801 aggatctcca acgtcgccgt catgccgacg cctcccggaa ttgaacccac tgacccggcg
     1861 tcagcagtgc gggcgggtcg cgctcgacgt cccacaacgc cgactgctct tcggacgtgt
     1921 ggccgatcag ctgccagccg cctggtgact cgcgggggta gacgccgctg aactcgcctg
     1981 ccagtccgac cgctccgatg ggaaccttgg ttcgaggttc cgaccgcctc ggtaccttga
     2041 gccgttcgtc gccgccgacc aggtaaccga aacccggtgc gaaaccggcg aaccccaccc
     2101 gccacggggt ctgcgtatgc gcggcgacga cttcgcgcgg cgtcagacct gtcaggtcag
     2161 cgacctcgcc gaggtcggcc ccctcgtaaa ccacatcgat caccacgtcg gcccggccgt
     2221 cgatcggcgc ggcggtctgc tcgaggcttt ccggtgccaa cctcagcttg ctgagctgtt
     2281 gcctggcgac gccctggtag cgcgggccat cgagcttgat gagcaccgtt ctcgaggccg
     2341 gaacgatgtc gacgacgccg agcaggtcgg cgtcccgaac gacgtcggcc cacgccaata
     2401 cctccgctgt gctgtcgaac tggagcagca ccgccgcatc gccgtagtcg gtgactctgc
     2461 cgatcaccat cgcgtccgtt gtcacggtca tctccc
//
LOCUS       DASYLX010000937         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2910573,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000937 DASYLX010000000
VERSION     DASYLX010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910573"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VGP27_21940"
     CDS             <1..1276
                     /locus_tag="VGP27_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV7853994.1"
                     /translation="TKPKSSAKDVVFPDQENSIWERDPKTNEWYLHHFYKHQPDLNIE
                     NPKVQEEISRTLGFWLELGVSGFRVDAVPFLFAKDAVPGSPDMFNPNQYLGDVRNFVT
                     RRVGDAVLLGEVNVPYKDQLAFFGGPDGDGLNMQFDFIGMQNIYLSLARGDARPIANA
                     LKQRPRLDVTSQWANFMRNHDELTLDKLSDDERQEIFDAFGPDKDMQLYGRGLRRRLP
                     SMLGGDEQRMKMAYSLAFSLPGTPVLFYGEEIGMAENLDIPGRLAVRTPMQWTGDLNG
                     GFSKAAKRRLCRPLPDGLYGPDRVNAADQRHDHQSFWWFMRNLIYTYRSQPEIGWSTL
                     DVLKQPHPAVLAHVCREESGWAMVALHNFGPDSVMVPIELPDAPAESTLVDLLDGLSE
                     HQFDAKGRIEVKLEPYGFRWLRLLRPQDDPII"
     gene            1300..1374
                     /locus_tag="VGP27_21945"
     tRNA            1300..1374
                     /locus_tag="VGP27_21945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1332..1334,aa:Thr,seq:tgt)
     gene            1419..1825
                     /locus_tag="VGP27_21950"
                     /pseudo
     CDS             1419..1825
                     /locus_tag="VGP27_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602231.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          422 a          671 c          617 g          354 t
ORIGIN      
        1 gaccaagccc aagtcgagcg caaaggacgt ggtcttcccg gaccaggaga acagcatctg
       61 ggaacgcgac cccaagacga acgagtggta cctgcaccac ttctacaagc atcaacccga
      121 tctcaacatc gagaatccca aagtgcagga agagatctcg cggacgctcg ggttctggct
      181 ggagctgggg gtgtccggct tcagggtcga cgccgtgccc tttctcttcg ccaaggatgc
      241 ggtgccggga agcccggaca tgttcaaccc gaaccagtac ctcggcgacg tccgcaactt
      301 cgtcacccgc cgcgtcggag acgccgtcct gctcggcgag gtcaatgtcc cgtacaagga
      361 ccaattggcg ttcttcggcg gccccgacgg cgacgggctc aacatgcaat tcgacttcat
      421 cggaatgcag aacatctacc tgtcgctcgc ccgcggcgat gcccgcccga tcgccaacgc
      481 cctcaagcag cgaccgcgcc tcgacgtgac cagccagtgg gccaacttca tgcgcaacca
      541 cgacgagctg acgctcgaca agctgagtga cgatgagcgg caggagattt tcgacgcgtt
      601 cggtcccgac aaggacatgc agctgtacgg tcgcggactg cgccgacggt tgccgtccat
      661 gctcggcggc gacgagcagc gcatgaagat ggcctactca ctggcattct cactcccggg
      721 tacccccgtc ctgttctacg gcgaagagat cggcatggcc gaaaacctcg atatcccagg
      781 caggttggcg gtgcgcacgc cgatgcagtg gacaggcgat ctgaacgggg gcttctccaa
      841 agcggccaaa cgtcgtctct gccgtcccct gcccgacggt ctctacggtc cggaccgggt
      901 caacgcggct gaccagcgac acgaccacca atccttctgg tggttcatgc gcaacctcat
      961 ctacacctac cggtcgcagc ccgagatcgg gtggtcaacc ctcgatgtgt tgaagcagcc
     1021 ccatcccgcg gtgctcgcgc acgtgtgccg cgaggaatcc gggtgggcca tggtcgccct
     1081 gcacaacttc ggtccggaca gcgtcatggt gccgatcgag ttgcctgacg cgccggccga
     1141 atcaacattg gtcgaccttc tcgacggcct atccgagcac cagttcgacg ccaagggccg
     1201 gatcgaggtc aaactcgagc cgtacggctt ccggtggctt cgcctgttgc gaccgcagga
     1261 cgacccgatc atctgacccg ataaactcgg agcccgcagg cctccttagc tcagtggtag
     1321 agcatcgctc ttgtaaagcg aatgtcatcg gttcaatccc gataggaggc tccacccagc
     1381 gtccgattcc tgtcggtggc cggtcgtagc ttgcatacat gaggcagtgc cgcggctgtg
     1441 gttcagagct ctcgaggcgc agccagaaga tttactgcgg caatgcctgt cagggttcgt
     1501 tccgacgcaa agccctgacg gaattgtggc tggagtcggg caaggcgcgc atcgacggcc
     1561 accaaggtca ctacatccgc gagtacctca ccgacgctca gtcgggctgc tgcgccatct
     1621 gtggtggggc cagtgtgtgg cttgacctgc ccctggcgtt ggtgttggac cacatcgatg
     1681 gggacccgac caacaatcga cgggggagaa cctgcggctg atctgtccga actgtgactc
     1741 acagttatcg acgtacaaaa gccggaaccg cggcaacggt cgcgccttcc gccgacagcg
     1801 atacgccaac ggtcagtcgt attgagcggc gaccagcgac gaaacgctgc cgacttaacg
     1861 cacaaggatg aaaccaccaa ccgcctagca ggaccagtac gcacctctcg ccagacggcg
     1921 cctgctagtc acgaggatgc ccgcggcgca atatgccaaa cctgggcacg ggccctgtct
     1981 cgatcggccc ccggaacaca cgatgattgc gtcggcacat acggggcaat atcaaccttc
     2041 acgaaggagg tgcagcaatg ggag
//
LOCUS       DASYLX010000938         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1154515,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000938 DASYLX010000000
VERSION     DASYLX010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154515"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VGP27_21955"
     CDS             <1..926
                     /locus_tag="VGP27_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV7853995.1"
                     /translation="NIHVEQVCANNLGLLELCRAPAIPVSKGADEPLCGRWPHHSKFH
                     GPTGLGYAKLPPTHRRLTDHDATAAWVAAARAHPGDLIGLVTGPLTNLALALRAEPAL
                     PRLLRRLVIMGGAFEYRGNTTPVAEWNISVDPEAAHEVFSVWSAAWGLDPPRHLPIVL
                     GLNLTENVAMTPALLGRLATEAGSATAPMSVLDERGTRSVAANPLIRVLEDAMRFYFE
                     FHFDQDEGYLAHLHDPLAAGVALDPELVTTRPATVDVELTGTLTRGMTIADWSGRWGR
                     EPNALIGIDVDPAAFFDRFIARVGHFARTLR"
     gene            1025..2512
                     /locus_tag="VGP27_21960"
     CDS             1025..2512
                     /locus_tag="VGP27_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEV7853996.1"
                     /translation="MRTLWQRPEPSPGPPSNPAEIADRALHRRAIETVIWGMPAVNYE
                     LMRRQMVKLGGAPNQVVFWSRPVSWKNQTLTPNPDTIYFMPFFDTRDVGPVVLEIPPA
                     DTGTIVGSIDDCWQTAIEDVGPAGADKGAGGKYLILPPNHDGGVPDGYLPCPSSTYQS
                     YALLRSNYRSVADADIAAAVAYGKRVRVYPLSAAGDAQQSTFIDAIDTLFDAKIPYDV
                     RFFDALNGIVQTEAWLVRDKAMIDTLKTIGIEKGLPFVPNDKTRRIFNLAAQEAHTWL
                     TNRLESSYFPPPYFDGGHWHVPASDEVLKGMMSNFADPDSYPVDNRGVTYSLAFFSAK
                     HLGTGQFYLMTLKDGKGNKLDGGTTYRLRVPASAPVRQYWSATAYDGKTHALMRDTPR
                     SSRASTSANLATNPDGSVDVFFGPTAPDGSSSNWIPTKACNTFEVIFRFYGPDQALFD
                     KTWKLPDIEKVPAHVRSEQEPAHVRSEQGPAHARSEQGPAHARSE"
     gene            complement(2542..>2852)
                     /locus_tag="VGP27_21965"
     CDS             complement(2542..>2852)
                     /locus_tag="VGP27_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEV7853997.1"
                     /translation="LPEPSPTVLLDDPVEVLDHEGNPVRVTNRGLFSADPARLAAPGR
                     EGVLSWWAGPWPVDERWWDPDRRAGRTARAQVLLDGDPGQALLLCYRQRRWYLEGIYE
                     "
BASE COUNT          524 a          975 c          891 g          462 t
ORIGIN      
        1 gcaacattca cgtggagcag gtttgcgcaa acaatctggg tttgctcgaa ttgtgccgag
       61 ccccagccat accggtgtcc aagggcgccg acgagccgct gtgcggccgg tggccccatc
      121 actcaaagtt ccacggcccc acgggattgg gctatgccaa gctgcccccc acccaccggc
      181 ggctcaccga ccacgacgcc accgcggcgt gggtggccgc ggcgcgcgcc catcccggcg
      241 acttgatcgg cctggtcacc ggcccgctga ccaacctggc gctggcgttg cgcgccgaac
      301 ccgcgctgcc gaggctgctg cggcggctgg tgatcatggg cggcgcgttc gagtaccgcg
      361 gcaacacgac gccggtcgcg gaatggaaca tcagcgtcga ccccgaggcc gctcacgagg
      421 tcttctccgt ctggtccgcc gcatggggcc tggatccgcc gcggcatctt ccgatcgtcc
      481 tgggactgaa cctgaccgag aacgtcgcga tgacacccgc gctgctgggc cggctcgcga
      541 cggaagcggg ctcggcgacc gcaccgatga gcgtgctcga cgaacgcggc acccggtccg
      601 ttgcggccaa cccgctgatc cgggtgctcg aagatgccat gcggttctac ttcgagttcc
      661 atttcgacca ggacgaaggc tatctcgcac acctgcacga cccgctggcc gcgggtgtgg
      721 cgctggatcc agagctggtg acgacgcggc ctgcgaccgt cgacgtcgag ttgaccggca
      781 cgctgacgcg cggcatgacg atcgccgact ggtcgggccg ctgggggcgg gaacccaacg
      841 cgctcatcgg cattgacgtc gatccggccg ccttcttcga ccggttcatc gcgcgggtcg
      901 gccatttcgc ccgaaccctc cggtgacgcc caaacgtacg tttgggcgga aaagtgcgag
      961 taaaagtctg caaaacgtcg atctcaagcg ttgaatagcg gcatcgcgac gtgggaggcg
     1021 ccccatgcgc acgctgtggc aacgaccgga accgtcgccg ggtccgccgt cgaacccggc
     1081 cgagatcgcc gaccgggcat tacatcggcg tgccatcgag acggtcatct ggggtatgcc
     1141 cgccgtcaac tacgagttga tgcgcaggca gatggtcaag ctcggcgggg cacccaacca
     1201 ggtggtgttc tggtcgcggc cggtgagctg gaagaaccag acactcaccc cgaaccccga
     1261 caccatttac ttcatgccat tcttcgacac cagggacgtc ggtccggtgg tgctggagat
     1321 tccgcctgcc gacaccggca ccatcgtcgg ctcgatcgac gactgctggc agacggcgat
     1381 cgaagacgtc gggcctgcag gcgccgacaa aggagcgggt gggaagtacc tcatcttgcc
     1441 gcccaaccat gacggcggtg tgcccgacgg ctaccttccg tgtccgtcgt ccacctatca
     1501 gtcctacgcg ctgttgcggt cgaactatcg cagtgtcgcc gacgccgaca tcgccgccgc
     1561 cgtggcatac gggaaacgcg ttcgggtgta tccgctttcg gcggccggcg atgcacagca
     1621 gtcgacgttc atcgacgcga tcgacacgtt attcgacgca aagatcccct acgatgtgcg
     1681 gtttttcgac gcactgaacg gcatcgtcca gaccgaggca tggctcgtcc gcgacaaggc
     1741 gatgatcgac actctgaaaa ccatcggcat cgagaaaggg ctgccgttcg tcccgaatga
     1801 caagacgcga cggatcttca atcttgcggc gcaagaagcg catacgtggc tgacgaatcg
     1861 gctggagtcg tcgtacttcc cgccgcccta cttcgacggt gggcactggc atgtgcccgc
     1921 ctccgacgag gttctgaagg gaatgatgtc gaacttcgcc gaccccgaca gctatcccgt
     1981 cgacaatcgc ggggtgacat actcactggc cttcttcagc gccaaacatc ttggcaccgg
     2041 gcagttctac ctgatgacgc tcaaggacgg caagggaaac aagctcgacg gcggaaccac
     2101 ctaccggctg cgggtacccg catcggcgcc cgtgcgccag tactggtcgg cgacggccta
     2161 cgacggtaag acgcatgccc tgatgcggga cactccgcgc tccagccggg cctccacctc
     2221 tgcgaacctg gcgacgaacc ccgacggttc ggtcgacgtc ttcttcggtc ccaccgcacc
     2281 ggacggttcg tcatcgaact ggataccgac caaggcctgc aacaccttcg aggtgatctt
     2341 ccggttctac gggccggatc aagcgctctt cgacaagacc tggaagcttc cggacatcga
     2401 gaaggttccc gcgcacgtga ggagcgaaca agaacccgcg cacgtgagga gcgaacaagg
     2461 gcccgcgcac gcgaggagcg aacaagggcc cgcgcacgcg aggagcgaat agggacccgc
     2521 gcacacgagt ccggccactg actactcgta gatcccttcc agataccacc gccgctggcg
     2581 gtaacacagc agcagcgcct gccctggatc tccatcgagc agcacctgag ctctcgccgt
     2641 gcgtccggcc ctgcggtccg gatcccacca ccgctcgtcg acgggccacg gccctgccca
     2701 ccacgacaac acaccttccc tgcctggggc ggccagccgc gccgggtcgg cggagaacaa
     2761 tcccctattg gtcacccgca ccggattgcc ttcgtggtca agcacttcga cgggatcgtc
     2821 gagcagcacg gtcggtgacg gttcgggcag tt
//
LOCUS       DASYLX010000939         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2800229,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000939 DASYLX010000000
VERSION     DASYLX010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800229"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGP27_21970"
     CDS             complement(<1..263)
                     /locus_tag="VGP27_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV7853998.1"
                     /translation="MFEGPVQDLIDELGKLPGIGPKSAQRIAFHLLSVEPPDIDRLTA
                     VLGKVRDGVKFCAVCGNVSDDDRCRICSDSRRDASLVCVVEEP"
     gene            complement(297..596)
                     /locus_tag="VGP27_21975"
                     /pseudo
     CDS             complement(297..596)
                     /locus_tag="VGP27_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
     gene            701..1450
                     /locus_tag="VGP27_21980"
     CDS             701..1450
                     /locus_tag="VGP27_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3717 family N-acetylmuramoyl-L-alanine
                     amidase"
                     /protein_id="HEV7853999.1"
                     /translation="MSAILRVGATAAACLVVAASTLAGPASAAPANIAGKIVFLDPGH
                     NGANDGSISRQVPTGRGGTKNCQESGTSTNDGYPEHSFTWETTLRIRQELSTLGVRTA
                     LSRGNDTALGPCVDERAAMANALKPNAIVSIHADGGPPTGRGFHVLYSSPPLNQVQAG
                     PSVQFAQTMRDQLRAAGLVPSTYIGTDGLNPRSDIAGLNLAQYPSILIELGNMKNPAD
                     AGLITTPEGRQKYADAVVRGIAGFLATQPVV"
     gene            complement(1454..1894)
                     /locus_tag="VGP27_21985"
     CDS             complement(1454..1894)
                     /locus_tag="VGP27_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7854000.1"
                     /translation="MGQVSAASSVLIDAAPEKVLAAVADYTTVRPKILSAHYRDYTVL
                     EGGQGAGTVASWKLQATKSRVRDVKADVDVAGHTVIEKDANSSMVTNWTVAPAGEGSS
                     VTVKTSWQGAGGIGGFFEKTFAPLGLKKIQAEVLHNLKKAVEAS"
BASE COUNT          325 a          782 c          626 g          350 t
ORIGIN      
        1 ttcggctcct cgacgacaca caccagcgac gcatcgcggc gcgaatccga acagatccgg
       61 caccggtcgt cgtcggagac gttgccgcac accgcacaga acttcacccc gtcgcgcacc
      121 ttccccagca cggcggtcaa ccggtcgata tccggcggtt cgacggacag cagatggaag
      181 gcgatgcgct gcgcactctt cggaccgatg ccgggcagct tgcccaactc gtcgatcaga
      241 tcctgaaccg ggccctcgaa caattacagc cccggtaaac ctaggtcgcc catgccaccg
      301 gccagcggcc cgagccgctc ctgggccagc aaggtgacct gacgcgccgc gtcggcgtgc
      361 gcacccacga tcagatcctg cagcgtttcg acgtcgtccg ggtcgacgac cttcggatcg
      421 atggacacgc ccaccacttc gccgctgccc ttcatggtga cttggaccag cccgccaccg
      481 gcctggccgt gcacctcggc gttggcgagc tgctcctggg cctccatcag cttctgctgc
      541 acctgttgcg cctgcgccag gagcgccgac atgtcgggtt ggcctccggg ttgcatgaca
      601 gtccccttgc gtattggtct cgacttggtt gcgctcgccg gcacgcggcg tttcggcctt
      661 tcaagcctag acgcccagcg gctaccttgg cccggtgcac gtgtcagcga tcctgcgtgt
      721 cggcgccacc gcggctgcct gcctagtcgt cgccgcgtcg acgctcgccg gccccgcatc
      781 cgccgcgccc gccaacatcg cgggcaagat cgtgttcctc gaccccggcc acaacggagc
      841 caacgacggc tcgatcagcc gccaggtgcc caccggccgc ggcggcacca agaactgtca
      901 ggaaagcggc acgtcgacca acgacggcta tcccgaacac tcgttcacct gggaaacgac
      961 gctgcggatc cgccaggagc tgtcgacgct gggcgtccgg accgcgctgt cccgcggcaa
     1021 cgacacggcg ctgggcccct gcgtcgacga gcgggcggcg atggccaacg cgctgaaacc
     1081 caatgccatc gtcagcatcc acgccgacgg cggtccgccg accggccgcg ggttccacgt
     1141 tctctactcg tcgccgccgc tcaatcaggt gcaggcaggg ccgtcggtgc agttcgccca
     1201 gaccatgcgg gatcagctgc gggcggccgg cctggtgccc tcgacgtata tcgggaccga
     1261 cgggctgaac ccccgctcgg acatcgccgg gctgaacctg gcgcagtatc cgtcgatcct
     1321 gatcgagctg ggcaatatga agaaccccgc ggacgcgggc ctgatcacca cacccgaggg
     1381 caggcagaaa tacgccgacg ccgtggttcg tggcatcgcg ggcttcctcg ccacccagcc
     1441 cgtcgtgtag ccgctagctt gcttccacgg ccttcttgag gttgtgcagc acctcggcct
     1501 ggatcttctt cagccccagc ggcgcgaatg tcttctcgaa gaagccgccg attccgcccg
     1561 cgccctgcca cgacgtcttg acggtgacgg acgagccctc tccggcaggt gcgacggtcc
     1621 agttggtcac catcgagctg ttggcgtcct tctcgatgac ggtgtggccc gcgacgtcca
     1681 cgtcggcctt gacgtcgcgc acccgagact tggtggcctg caacttccag ctcgccacgg
     1741 tgcccgcgcc ctggccaccc tcgagcactg tgtaatcgcg gtaatgcgcc gagaggatct
     1801 tcggccgcac cgttgtgtaa tccgccacag cagccaacac cttctccggc gctgcgtcga
     1861 tcaagacgct gctggccgcg ctcacctgtc ccatctcctg tgccttccct catcgtggtg
     1921 cggtcgcatc ggccgcaacc gcggactagc gtatatgcgt gtctgttccg gcaaccgacg
     1981 cacgtgctgc ccacgccgcc ggcgtgcagc ggctgcttgc cagctaccgt gccatccctc
     2041 cgacggccac cgtccgcctc gccaaaccga cgtccaacct gtt
//
LOCUS       DASYLX010000940         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2144033,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000940 DASYLX010000000
VERSION     DASYLX010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144033"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VGP27_21990"
     CDS             <1..867
                     /locus_tag="VGP27_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV7854001.1"
                     /translation="RTPFAYEGLFGHLAACAVPGVEEVRDGAYRRTLRLPAGNGIVSL
                     TPEPGQVRCTLVLDDFRDLATAIARCRRLLDLDADPEAVVDALSADAELSALVRKAPG
                     QRIPRTVDEAELALRVVLGQQVSVKAARTHAARLVTAYGRPLTDAGGGLTHVVPSIEH
                     LAEIDPIHMAFPRSRQRTLVSLVEALANRDVLLDAGCDWDRARHQLLALPGIGQWTAE
                     VIAMRGLGDPDAFPASDLGVLLAAKQVGLPEDPRALAQHSTRWRPWRSYAAQHLWTAL
                     DHAVNRWPPKEK"
     gene            867..1370
                     /locus_tag="VGP27_21995"
     CDS             867..1370
                     /locus_tag="VGP27_21995"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV7854002.1"
                     /translation="MSVIQFRSVDSPVGLLTLAGRDGRLMHLRMVDQTYEPSHNGWTP
                     DDGAFPDAVEQLESYFAGELFDFELNLDLVGTKFQRSVWAALLTIPYGQTRSYGEIAA
                     QIGSPGAFRAVGLANGHNPIGIIVPCHRVIGANGSLTGYGGGIDRKRALLGFERSRKP
                     VTPTLFD"
     gene            complement(1435..1548)
                     /gene="rrf"
                     /locus_tag="VGP27_22000"
     rRNA            complement(1435..1548)
                     /gene="rrf"
                     /locus_tag="VGP27_22000"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          254 a          526 c          502 g          283 t
ORIGIN      
        1 cgcaccccgt tcgcctacga gggtctgttc ggtcacctgg cggcctgcgc cgtgcccggg
       61 gtcgaggagg tccgcgacgg ggcgtaccgg cgaacgctgc ggttgcccgc aggcaacgga
      121 atcgtcagcc tgacaccgga acccggccag gttcggtgca ccctcgtgct cgacgacttc
      181 cgggacctcg ccaccgcaat cgcccgctgc aggcgcctac tggacctcga cgccgacccc
      241 gaagccgtcg tcgacgcgct gagcgccgac gcggagctga gcgcacttgt cagaaaggcg
      301 ccgggccaac gcatccctcg taccgtcgac gaagccgagc tggcgctgcg ggtggtgctt
      361 ggccagcagg tgtcggtcaa ggcggcccgc acccacgccg cccgcctcgt taccgcgtac
      421 ggccggccgc tgaccgatgc gggcggcggg ctgacacacg tcgtcccgtc gatcgaacat
      481 ctcgccgaga tcgacccgat tcacatggca tttcccagat cacgacagcg aaccctggtg
      541 tcgctggtcg aagcgctggc caaccgcgac gtgcttctgg atgccggctg cgactgggac
      601 cgcgctcgcc atcagctgtt ggcactgccg gggatcggac agtggacggc ggaggtcatc
      661 gccatgcgcg gcctggggga ccccgacgcg ttccccgcca gcgatctcgg cgtcctgctg
      721 gccgccaagc aggtgggctt gccggaggac cctcgtgctc tcgcccagca cagcactcgc
      781 tggcggccat ggagatccta tgccgctcaa cacctctgga ccgcccttga ccatgcggtg
      841 aaccgctggc cgccgaagga gaaatgatgt cagtgatcca gttccgatca gttgacagcc
      901 cggtcgggct gctcactctc gccggtcgcg acggtcgact gatgcatctg cgcatggtcg
      961 atcaaactta tgagccgagc cacaacggct ggacgcctga cgacggcgcg ttccccgatg
     1021 ccgtggagca actcgagtcc tacttcgccg gcgagctttt cgacttcgaa ctgaacctcg
     1081 acttggtcgg caccaaattt caacgcagcg tctgggcggc tttgttgacg atcccgtacg
     1141 ggcagacccg atcgtatgga gagatcgccg cacagatcgg atcgcccggc gcgttccgcg
     1201 cggtgggact ggccaacggc cacaacccga tcggcatcat cgtgccttgc catcgggtga
     1261 tcggcgcgaa tggcagcctc acaggatacg gcggcggcat cgaccgcaag cgtgcattgc
     1321 tcggattcga gaggagccgg aaaccggtga ctccgacgct attcgactga ctcggttgac
     1381 cccacaaatg cccttgcccc ccaatagaat tggggggcaa gggtctgatg ttgtgttcgg
     1441 cggtgtccta cttttccacc cgggttgggt agtatcatcg gcgctggcag gcttagcttc
     1501 cgggttcggg atgggtccgg gcgtttccct gtcgctattt ccgccgtaac tttttttcac
     1561 ttgtg
//
LOCUS       DASYLX010000941         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1264071,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000941 DASYLX010000000
VERSION     DASYLX010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264071"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGP27_22005"
     CDS             <1..504
                     /locus_tag="VGP27_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7854003.1"
                     /translation="LGSASKSLSQALRLGWMVLPDNLIDAVTDAAGGQQYYVDSLSQL
                     TMADFIGTGSYDKHIRRMRMRYRRRRDALVDALSEFDVNISGLSAGVNLLLTLPDDTE
                     HEVLRRAGEAGISVQGLSIMRHPLAGPEVPDPDGIIVGFAAPADHAFASAVDALLAVL
                     KASGLAQ"
     gene            535..1011
                     /locus_tag="VGP27_22010"
     CDS             535..1011
                     /locus_tag="VGP27_22010"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7854004.1"
                     /translation="MGVYERTLEKFARMPVGDWYMKRVAPRLDPPLLRLSGGRVSSVY
                     PVPIMLLTTTGAKSGQPRTLPLLYVTDGDRLILIASNYGNTSHPAWYRNLVANPKAEV
                     LAGKRSGTYSAAEITDPVERDRAWDLALDMYAGYGDYEGRAGDRRIPLVRLERTTG"
     gene            1093..>1700
                     /locus_tag="VGP27_22015"
     CDS             1093..>1700
                     /locus_tag="VGP27_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7854005.1"
                     /translation="MFDSMFAAASDEELVAAIEDGVRQEAIAGARRLSAIAELTRRRI
                     EDDERAMWAFDPWDSAAAEVAAAMAIGARRASGQMRIAEALREHLPQVAALHCKGALS
                     TRLVATITWGTRLVQGDEAWANIDAAIAASARTWERLSEDKLRAAVEVCVARYDPDAK
                     RRTDTNLRGRDFVIGACDDDTETTAVWGRLPAADAAVMKHRVT"
BASE COUNT          272 a          543 c          602 g          283 t
ORIGIN      
        1 ctcggatcgg ccagcaagag cctgtcgcag gcgttgcggc tgggctggat ggtattgccg
       61 gacaacctga ttgacgcagt gacggacgct gcgggagggc agcagtacta cgtcgactcg
      121 ctctcgcagc tgacgatggc ggacttcatc ggcaccggca gctacgacaa gcacatcagg
      181 cgcatgcgga tgcgctatcg cagacggcgc gacgccctcg tcgacgccct gtctgaattc
      241 gacgtcaaca tcagcgggct gtcggctggc gtgaacctgc tgctgactct gcctgacgac
      301 accgagcacg aggtgctccg tcgcgcgggt gaggcaggaa tttccgtgca gggtctgtcg
      361 atcatgcgtc atccgctggc cgggccggaa gtgcccgatc ccgacgggat catcgtcggc
      421 ttcgcggcgc cggccgacca cgcgttcgcc tcggccgttg atgcgctttt ggccgtgctg
      481 aaggcctccg ggctggcaca gtaatgcgct atgagtccag agcagcgtcg ccacatgggt
      541 gtgtacgagc gaacgctgga gaagttcgcg cgcatgcccg tcggtgactg gtacatgaag
      601 cgggtggcgc cccgcctgga tccgcctctg ctgcgcctct cgggcggacg ggtcagttcc
      661 gtgtatccgg ttccgatcat gctgctgacg acgacaggcg ccaagtcggg ccagccgcgg
      721 acgctgccgc tgctgtacgt caccgacggc gaccggctga tcttgatcgc gtcgaattac
      781 ggcaacacgt ctcacccggc gtggtaccgc aacctcgtcg cgaacccgaa ggcggaggta
      841 ctcgcgggca agaggagcgg tacctattcg gccgccgaaa tcaccgaccc cgtggagcgt
      901 gatcgcgcgt gggacctcgc actcgacatg tacgcgggct acggggacta cgagggcagg
      961 gcgggcgacc gccggattcc tttggtccgg ttggagcgca cgaccggctg agttattcac
     1021 agtcgtaatt tcatccacag gtacgggttg agctgctggt tccggtcggc ggtggtgggt
     1081 agcgtcgaac ctatgttcga ttcgatgttc gcggccgcct ctgatgagga gctggtggct
     1141 gccatcgagg acggggtccg gcaggaggcg atcgccggtg cgcgccggtt gtccgcgatc
     1201 gccgagctga cccggcgccg catcgaggac gacgagcgcg cgatgtgggc gttcgacccg
     1261 tgggattcgg cggccgccga agtggccgcg gccatggcga tcggtgcgcg ccgcgcgtcg
     1321 gggcagatgc ggatcgccga agcgctgcgc gagcatctgc cccaggtcgc cgccctgcac
     1381 tgtaagggcg cgttgagcac ccggctggtt gcgacaatca cctggggcac ccggctggtg
     1441 caaggcgacg aggcctgggc caacatcgac gccgcgatcg cggcaagcgc ccgcacctgg
     1501 gagcgcctgt ccgaggacaa gctgcgcgcc gccgtggaag tgtgtgtggc ccgctatgac
     1561 cccgacgcca aacgtcgcac cgacaccaat ctgcgcggcc gcgatttcgt catcggcgcc
     1621 tgcgatgacg acaccgagac cactgcggtg tgggggcggc tgccggccgc cgacgccgca
     1681 gtgatgaaac accgcgtcac
//
LOCUS       DASYLX010000942         5194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3460352,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000942 DASYLX010000000
VERSION     DASYLX010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3460352"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /gene="ettA"
                     /locus_tag="VGP27_22020"
     CDS             <1..1566
                     /gene="ettA"
                     /locus_tag="VGP27_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEV7854006.1"
                     /translation="VGPNGAGKSSVLRIMAGLDQANNGDAYLQPGATVGILMQEPQLD
                     ETKTVRENVEEGVAVKAKLNRFNEVAELMATDYTDELMEEMGVLQEELDAADAWDIDS
                     QLEQAMDALRCPPPDEPVTHLSGGEKRRVALCKLLLSKPDLLLLDEPTNHLDAESVQW
                     LEQHLAEYKGAILAVTHDRYFLDNVAEWILEIDRGRAYPYEGNYSTYLEKKAERVAVQ
                     GRKDAKLQKRLQEELAWVRSGAKARQAKSKARLGRYEEMAAEAEKTRKLDFEEIQIPV
                     GPRLGNIVVEVDHLDKGYDGRPLIKDLSFTLPRNGIVGVIGPNGVGKTTLFKSIVGLE
                     QPDSGTVKVGETVRLSYVDQSRGGIDPKKTVWEVVSDKLDYIEVGQTEIPSRAYVSAF
                     GFKGPDQQKPAGVLSGGERNRLNLALTLKVGGNLILLDEPTNDLDIETLSSLENALEN
                     FPGCAVVISHDRWFLDRTCTHILAWEGDDDNDAKWFWFEGNFGAYEENKVERLGIDAA
                     RPHRVTHRRLTRD"
     gene            1621..>5194
                     /locus_tag="VGP27_22025"
     CDS             1621..>5194
                     /locus_tag="VGP27_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEV7854007.1"
                     /translation="MTVNPGAGAIDGQTELMVAPTTLDKLARAYIATYHGPHGDAPTA
                     EATTGPVDMSAAGNLMSPNLLAAHYKLGRRRQVGETLVELYAKDDPGGFGPAIQIVTD
                     YATLLMDSVTVLLHRLGAAYKAIMNPILRVRRGPTGELVDIQPAPNAPSSRDTIDEAW
                     IHVQLSSSADPKVVAEVKRLLPGVLADARQVALDSRALNGTLLGLANEIDTDPEGRFP
                     GADRKDVAELLRWLADGHFVLLGYQRCPVHDGDSSVDMSSRLGILRFRDEILPQLTDS
                     DDLLVLAQATIPSFLRYGAYPYIVVVREHPGHHAIEHRFVGLFTVAAMNANVMDIPLI
                     ARRVGEAIAMAQRDPSHPAQLLLDIIQTIPRSELFALSARELLNMAMAVVDLGSRRRT
                     LLFLRADQLAHFVSCLVYLPRDRYTTAVRLDMQDILVRELGGESIEYTARVSESPWAT
                     VHFTVRMPEGSRRQSVDVSLQNETRIQDLLTEAARTWGDRLLGAVKTGSIDQVKAEHY
                     STAFPEVYKQAVTPEDAINDIAIIEDLQDNSVKLLFSEGGADQRSTTGDVKDGAAQLT
                     WYLGGRSASLSQLLPMLQSMGVIVLEERPFTVTRPDGLAVWIYQFKIAVLDDIPEDPE
                     GPERDATAVRFADAVTAIWHGRAEIDRFNELVLRARLSWRQVAILRAYAKYLRQAGFP
                     YSQTHIENVLNGNPGTSRSLVELFEALFDPAADPERKAAQAAVASVTADIDALTSLDT
                     DRVLRGFASMIQATLRTNYFVSRDDSARAQNVLSFKLNSALIDELPLPRPMFEIFVYS
                     PRVEGVHLRFGFVARGGLRWSDRKEDFRTEILGLVKAQAVKNAVIVPVGAKGGFVVKR
                     PTPLTGDPAVDRDTQRNDGIACYKLFISGLLDLTDNVDQTTGKVVTPADVVRRDGDDA
                     YLVVAADKGTATFSDIANEVAKSYGFWLGDAFASGGSVGYDHKQMGITAKGAWESVKR
                     HFREMGVDTQSEDFTVVGVGDMSGDVFGNGMLLSQHIRLVAAFDHRHVFLDPNPDAAT
                     SWEERKRMFELPRSTWDDYNKSLISEGGGVYSREQKSIPISPQVRTALGIENDVEELP
                     PPDLLRRILKAPVDLLWNGGIGTYIKAETESDADVGDRANDMIRVNGNQVRAKVIGEG
                     GNLGVTPLGRVEFDLAGGHINTDALDNSAGVDCSDHEVNIKILID"
BASE COUNT          957 a         1703 c         1688 g          846 t
ORIGIN      
        1 gtcggtccga acggggcggg aaagtcgagc gtcctgcgga tcatggccgg cctggaccag
       61 gccaacaacg gcgatgccta cctgcagccg ggcgcgacgg tcggcatcct gatgcaggaa
      121 cctcagctcg atgagaccaa gaccgtccgc gagaacgtcg aggagggcgt ggcggtcaag
      181 gccaagctca atcggttcaa cgaggtcgcc gagctgatgg cgacggacta caccgacgag
      241 ctgatggagg agatgggcgt acttcaggag gagctggacg ccgccgacgc ctgggacatc
      301 gactcacaac tcgagcaggc gatggacgcg ctgcgctgcc cgccgcccga cgaaccggtc
      361 actcaccttt ccggtggcga gaagcgacgc gtggcactgt gcaagctgct gctgtccaag
      421 cccgacctgc tgctgctcga cgagcccacc aaccacctcg acgccgagag tgtccagtgg
      481 ctcgagcagc acctcgccga gtacaagggc gccatcctgg ccgtcacgca cgaccggtat
      541 ttcctggaca acgtcgccga atggatcctc gagatcgacc gcggccgggc ctacccgtac
      601 gagggcaact actcgacgta cttggagaag aaggccgagc gcgtcgccgt ccagggccgc
      661 aaggacgcca agctgcagaa gcggctgcag gaggagctcg cctgggtgcg ttccggtgcg
      721 aaggcgcggc aggccaagag caaggcccgc ctcggccggt atgaggagat ggccgcggag
      781 gcggagaaga cgcgcaagct cgacttcgag gagatccaga ttccggtcgg tccgcgactt
      841 ggcaacatcg tcgtcgaggt ggatcacctc gacaagggct acgacggtag gccgttgatc
      901 aaggacctgt ccttcaccct gccgcgcaac ggcatcgtcg gcgtcatcgg tcccaacggc
      961 gtcggcaaga ccaccctgtt caaatccatc gtcggcctgg aacagcccga cagcggaacc
     1021 gtcaaggtcg gcgagaccgt caggctcagc tacgtcgacc agagccgcgg cggcatcgac
     1081 ccgaagaaga cggtgtggga ggtcgtctcc gacaagctgg actacatcga ggtcggccag
     1141 accgagatcc cgtcgcgggc ctacgtgtcg gcgttcgggt tcaaaggtcc cgaccagcag
     1201 aagccggcgg gcgtgctgtc cggcggtgaa cgcaaccggc tcaatttggc gctcacgctc
     1261 aaggtgggtg gaaacctcat tctgctcgac gagcccacca acgacctcga tatcgagacc
     1321 ctgagctcgc tggagaacgc gctggagaac tttcccggct gcgccgtggt gatcagccac
     1381 gaccgttggt tcctcgaccg cacctgcacg cacatcctcg cctgggaggg cgacgacgac
     1441 aacgacgcca agtggttctg gttcgagggc aacttcggtg cttacgaaga gaacaaagtg
     1501 gaacgacttg gtatcgatgc ggcccgtcct cacagggtga cccaccgccg gctgacacgc
     1561 gactagtgtc gccactgcgt gccaacgacg gcgtgcggcg gtgagtcagg agcgagcggc
     1621 atgacggtca atccaggggc gggggctatc gacgggcaga ccgaactgat ggtcgccccg
     1681 accaccctgg acaaactcgc cagggcctat atcgcgacct atcacggacc gcacggcgac
     1741 gctcctaccg ccgaggcgac caccggcccc gtcgatatgt ctgcggcggg caatctgatg
     1801 tcgccgaatc tgctggccgc gcactacaag cttggcaggc ggcggcaggt cggggagacg
     1861 ctcgtcgagc tctatgccaa agacgacccc ggcgggttcg gtccggcgat tcagatcgtc
     1921 accgactacg cgacgctgct gatggactcg gtgaccgtcc tgctgcaccg gcttggggcg
     1981 gcctacaagg cgatcatgaa tccgatcctg cgggtgcggc gcggtccgac cggtgagctg
     2041 gtcgacatcc agccggcgcc caatgctccc agttcgcggg acaccatcga tgaggcgtgg
     2101 attcacgtcc agttgtccag ttccgccgac cccaaggtcg tcgcggaggt caagcggctg
     2161 ctgccgggcg tgctcgccga tgcccgtcag gtcgcgctcg actcccgcgc gttgaacggg
     2221 acgttgcttg gcctcgccaa cgagatcgat accgacccgg aggggcggtt ccctggcgcc
     2281 gaccgcaaag acgtcgccga gctgctgcgc tggcttgccg acggccattt cgtgctgctc
     2341 ggctaccagc ggtgtccggt gcacgacggg gattcgtcgg tcgacatgtc gagccgactc
     2401 ggcatccttc ggttccgcga cgagatcctc ccgcagctga ccgacagcga cgacttgctg
     2461 gtgctggcgc aggccaccat tccgagcttc ctgcgttacg gcgcctaccc gtacatcgtc
     2521 gttgtccgcg agcaccccgg ccatcacgcg atcgagcacc gcttcgtcgg cctgttcacc
     2581 gtcgcggcca tgaacgccaa tgtgatggat ataccgctga ttgctcgccg agtcggcgaa
     2641 gccatcgcga tggcgcagcg cgatcccagc catccggctc aactgttgct cgacatcatc
     2701 cagaccatcc cgcggtcgga gctctttgcg ctgtccgcac gcgagctgct gaacatggcg
     2761 atggccgtcg tcgacctggg ctcgcgtcgg cgcacgctgc tattcctgcg tgccgaccag
     2821 ctcgcgcatt tcgtgtcctg cctcgtctat ctgccacgag accggtacac caccgcagtc
     2881 aggctcgaca tgcaggacat cctggtgcgc gagctgggtg gcgagagcat cgaatacacc
     2941 gcccgggtca gtgaatctcc ctgggcgacg gtgcatttca ccgttcgcat gcccgagggg
     3001 tcccgtcgcc agagcgtcga cgtctcgctg cagaacgaga ctcgcatcca agacttgctg
     3061 accgaggccg cgcggacgtg gggcgaccgg ttgctcggag cggtcaagac gggatcgatc
     3121 gatcaggtca aggccgagca ctattcgaca gcctttcccg aggtctacaa gcaggccgtc
     3181 acacccgagg acgcgatcaa cgacatcgcg atcatcgaag atctgcagga caattcggtg
     3241 aagctgttgt tctccgaggg cggcgcggac cagaggagca ccacagggga tgtcaaggac
     3301 ggtgcggcgc agctgacttg gtatctcggc gggcggtcgg cctcgctgag ccagttgctg
     3361 cccatgctgc agtccatggg cgtgatcgtg ctcgaggagc ggcccttcac cgtcacccgg
     3421 cccgacgggc tggcggtgtg gatttaccag ttcaagatcg cggtgctcga cgacatcccc
     3481 gaggaccccg aggggcccga gcgggatgcg accgcggtgc ggttcgccga cgcagtcact
     3541 gcgatctggc acggccgtgc cgagatcgac cggttcaacg agttggtgct gcgggcacgg
     3601 ctgtcgtggc ggcaggtcgc catcctccgc gcgtacgcca agtacctgag gcaggcggga
     3661 tttccctaca gtcagaccca tatcgagaac gtgctcaacg gcaacccggg cacgtcgcgg
     3721 tcactggttg agctcttcga agcccttttc gacccggccg ccgatcccga gcggaaggcc
     3781 gcgcaggcgg ccgtagcctc ggtgaccgcc gacatcgacg ccctgaccag tttggacacc
     3841 gatcgtgtgc tgcgcggatt cgcgtcgatg atccaggcca cgctgcgcac caactacttt
     3901 gtcagccgcg acgattccgc ccgcgcgcag aatgtgctct ccttcaagct gaactctgcg
     3961 ctgatcgacg aactgccgct gccgcggccg atgttcgaga tcttcgtcta ctcgccccgg
     4021 gtcgaaggcg tgcatctgcg gttcggcttc gtcgcccgcg gcgggctgcg atggtcggat
     4081 cgcaaggaag acttccgcac cgagatcctc ggcctggtca aggcgcaggc cgtgaagaac
     4141 gccgtcatcg ttcccgtcgg ggccaagggc ggttttgtcg tcaaacggcc cacaccactc
     4201 accggagatc ccgccgtcga tcgcgatacg cagcgaaacg acggcatcgc ctgctacaag
     4261 ctgttcatct cgggcctgct cgacctgacc gacaacgtcg accagaccac tgggaaagtc
     4321 gtcacgccgg cagatgtcgt gcgccgcgac ggcgacgacg cgtacctcgt ggtggctgcg
     4381 gacaagggca ccgcgacgtt ctccgatatc gccaacgagg tggcgaagtc ctacggcttc
     4441 tggctcggcg acgcattcgc gtccggcggt tcggtcggct acgaccacaa acaaatgggc
     4501 atcaccgcca agggcgcatg ggagagcgtc aaacggcact tccgcgagat gggcgtcgac
     4561 acccagtccg aggacttcac cgttgtcggt gtcggcgata tgagcggcga cgtgttcggc
     4621 aatggaatgc ttctgtccca gcacattcgg ctggtcgcgg ccttcgacca ccgacacgtg
     4681 ttcctcgacc cgaacccaga cgcggcgacg tcgtgggagg agcgcaagcg gatgttcgag
     4741 ctgccgcgtt cgacctggga cgactacaac aagtccttga tcagcgaggg cggcggcgtg
     4801 tacagccgcg agcagaagtc gatcccgatc agcccgcagg tgcgcaccgc cctcggcatc
     4861 gagaacgacg tcgaggaact gccgccgccc gacctgttgc gccgcatcct gaaggcaccg
     4921 gtggatttgc tatggaacgg cggcatcggc acctacatca aggccgaaac cgagtccgac
     4981 gccgacgtcg gcgaccgcgc caacgacatg atccgggtca acggcaatca agtgcgtgcc
     5041 aaggtgatcg gcgagggcgg caatctcggc gtcacaccgc tggggcgcgt cgagttcgac
     5101 ctcgccggcg gtcatatcaa caccgacgcg ttggacaact ccgccggcgt cgactgttcc
     5161 gaccatgaag tgaacatcaa gattctgatc gacg
//
LOCUS       DASYLX010000943         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3790634,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000943 DASYLX010000000
VERSION     DASYLX010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3790634"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGP27_22030"
     CDS             complement(<1..648)
                     /locus_tag="VGP27_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7854008.1"
                     /translation="MESLTVTANLTMLRVNGWQLYVWRDGDSATLIDTGAPASGAEIA
                     AAVPGIDRIVLTHGHVDHTGSAAELRESTGALVIAGTGDAAVIRGEAAMPPPAFEDWE
                     LPIHERVSADLPAVAPAVPVDLELNDGDVLDFGGGAHVLAIPGHTEGSIAVHLPEHGV
                     LFAGDTIANVGSVMLGVFNQDRARAVASFKRLAALDVATACFGHGDPIDTGAGDRI"
     gene            875..1315
                     /locus_tag="VGP27_22035"
     CDS             875..1315
                     /locus_tag="VGP27_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854009.1"
                     /translation="MNTNTSRGLIGFVAAPVTAAAVMAGAVGFASVAHAGVTPGVNVH
                     ASRMVLSRESLAQQKQVHGKSAAALDQQRQQEEKQSDSPAKQEASELKGDLKAEQVKQ
                     TALKTVESMANVQPAEPKAHTYALKTPIHKPVSLMRAGFSQMAR"
     gene            complement(1327..2973)
                     /locus_tag="VGP27_22040"
     CDS             complement(1327..2973)
                     /locus_tag="VGP27_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEV7854010.1"
                     /translation="MGRRTTAVDDVAERYLATYAALDPCAATEMGIVGHAVNYETDLT
                     DYSPDGATARADAARSALRELDGVKAEDDVDVVTLAAMRERLGVQLELHDAGLDVGEL
                     NVIASPLQSMRDVFDLMPDDTEDDWRVIAERLSRVPDRVAGYAESLRAAVAAGHSPAV
                     RQVGRGIQQAGQIQQLFCDMVAKAVPGNEVLHADLQARASAAADAYSRLAAVLRDEVG
                     PHARGEDAFGRDAYRLYSRLYLGAEVDLDETYEWGLRQLEAIVAEQESIAHRLYPGSS
                     VAEALSRLDEEPRYVVEGTDTLRAWMQELSDRVVDALAHKHFEIAEPLRNLECMIAPT
                     HTGGIYYTGPSEDLTRPGRMWWSVPPGVDTFHTWQETTTVFHEGVPGHHLQIGRAVVL
                     ADQLNRWRRLGCWVSGHGEGWALYAERLMAELGWLDDAGNRMGMLDAQRFRAARVVID
                     IGVHCGMTAPDGGTWDADKAWVFLQAHSAMKEAKLRFELDRYLGWPGQAPSYAVGQRI
                     WQQLRDELVCRGTSLKEFHRRALDLGGLPLDVLRAALSAS"
     gene            complement(2966..>3407)
                     /locus_tag="VGP27_22045"
     CDS             complement(2966..>3407)
                     /locus_tag="VGP27_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV7854011.1"
                     /translation="RTQFGSSGGPFAQEYVVAHEFGHHVQNLQGTLGRAQQGAQGATG
                     NGVRTELQADCYAGVWAHYASTTKQEGTDVPFLEPLSDKDIADALSAAASVGDDRIQK
                     QATGRVNPESWTHGSSEQRQHWFTVGYQTGDPNKCDTFAARDLG"
BASE COUNT          586 a         1149 c         1105 g          567 t
ORIGIN      
        1 gatccgatca cccgcgccgg tgtcgatggg gtcgccgtgc ccgaagcacg ccgtcgcaac
       61 gtccagcgcc gccaagcgct tgaacgatgc gaccgcgcgc gcccgatcct ggttgaaaac
      121 cccgagcatc accgatccga cgttggcgat ggtgtcgccc gcgaacagca cgccatgttc
      181 gggtaagtgc accgcgatgc tgccctcggt gtgcccgggt atcgcgagga cgtgtgcacc
      241 gccaccgaag tccagcacgt cgccgtcatt cagctcaagg tctaccggga cggcaggagc
      301 gacggcaggc aagtccgccg aaacccgttc gtggataggg agttcccagt cttcgaacgc
      361 gggtggcggc atggcggctt caccgcggat gactgcagca tctcctgtgc cggctatcac
      421 aagtgccccg gtcgactcac gcagttctgc ggccgaaccg gtgtggtcga cgtggccgtg
      481 cgtcaacacg attcggtcga tgccgggcac ggcggccgct atctcggcgc ccgatgccgg
      541 tgctccggtg tcgatgaggg tcgccgagtc gccgtcgcgc cagacgtaca gctgccaccc
      601 gttgacgcgc agcatggtca ggttcgcggt gacggttagt gattccatct ctcaacgcta
      661 ggtcaaggga acacgctggg ttgacgactt cgccgtcggc gaacttcgct gttgcaaaac
      721 agatttcgga accgacgtct ctgcgagcga aatgcgcaaa agtcggtgaa cagaggttat
      781 gcgggttgtg cgcacaagcg agccaccgcg ccgtgagagc catagcttcg tcgtaccagt
      841 aacagacaca caggagcaca tacaggagct agaaatgaac accaacacat ctcgtggcct
      901 catcggcttc gtcgccgccc ccgtcaccgc cgcggccgtc atggcgggtg cagtgggatt
      961 cgccagtgtc gcccatgccg gcgtcacacc cggcgtcaat gtgcacgcgt cccggatggt
     1021 gctgtcgcgg gagtcccttg cgcagcagaa gcaggtgcac ggtaagagcg cggctgcgtt
     1081 ggatcagcag aggcagcagg aggaaaagca gtccgacagt cccgccaagc aggaagcttc
     1141 cgaactcaag ggcgatctga aggctgagca ggtgaagcag acggcgctca agacggtcga
     1201 gtcgatggcg aatgtgcagc cggccgagcc caaggcgcat acgtatgcgc tgaagactcc
     1261 gatccacaag ccggtgagtc tgatgcgcgc cggcttctca cagatggctc gatgaaagcg
     1321 ccaaactcag ctcgccgaca gcgccgccct gagcacgtcg agcggcaggc cgcccagatc
     1381 cagtgcgcgg cggtggaact ccttcaacga ggttccgcga cataccaact cgtcacgcag
     1441 ctgttgccag atccgctggc cgaccgcata tgacggcgcc tggcccggcc agccgaggta
     1501 gcggtccagc tcgaagcgga gcttggcctc cttcatcgca ctgtgcgcct gcaggaacac
     1561 ccatgccttg tcggcgtccc atgtgccgcc gtcgggggcg gtcataccgc agtgcacgcc
     1621 gatgtcgatg accacgcggg cggcgcggaa tcgttgagcg tcaagcattc ccatgcggtt
     1681 gcctgcgtcg tcgagccagc cgagctccgc catcagccgc tccgcataca acgcccagcc
     1741 ctcgccatga cccgacaccc agcagcccag ccgccgccag cggttcagct ggtcggccag
     1801 cacgacggca cgaccgatct gaaggtgatg gccgggaacg ccttcatgga agaccgtcgt
     1861 ggtctcctgc caggtgtgga aggtgtcgac gcccggcgga accgaccacc acatccgtcc
     1921 cggccgggtg agatcctctg acgggccggt gtagtagata ccgccggtgt gcgtcggtgc
     1981 gatcatgcac tcgagattgc gcagcggctc ggcgatctcg aagtgcttgt gggccagcgc
     2041 atcgacgacg cgatcggaca gttcctgcat ccacgcccgc agtgtgtcgg tgccttccac
     2101 gacgtaccgc ggttcctcgt cgagccggct cagcgcctcg gccaccgacg aaccggggta
     2161 gagccggtgg gcgatggact cctgttccgc cacaatcgct tccagctggc gcagccccca
     2221 ttcgtaggtc tcatcgagat ccacctccgc accgagatag agcctcgagt acaggcggta
     2281 cgcgtcgcgg ccgaacgcgt cctcgccacg ggcgtgcggg ccgacctcgt cgcgcagcac
     2341 ggccgcgagc ctgctgtacg cgtcggcggc cgccgacgcc cgcgcttgca ggtcggcgtg
     2401 cagcacctcg ttgccgggca ccgccttggc caccatgtcg cagaacagct gctggatctg
     2461 gcccgcctgc tggatgcctc ggcccacctg tcgaaccgcg ggcgaatggc cggccgcgac
     2521 cgcggcgcga agcgactcgg catagcccgc aacgcgatcg ggtacccggg acagccgctc
     2581 ggcgatcacc ctccaatcgt cctcggtgtc atcgggcatc aggtcgaaca cgtcccgcat
     2641 cgactgcagc ggcgacgcga tcacgttcaa ctcaccgacg tcgaggccgg catcgtgcaa
     2701 ctcgagctgg actccgagtc gctcccgcat cgcggcgagt gtcacgacgt cgacgtcgtc
     2761 ctccgccttg acgccatcga gctcacgcag tgcgctgcgg gcggcatcgg cacgcgccgt
     2821 cgcaccgtcg ggggagtagt cggtgaggtc ggtttcgtag ttgacggcgt ggccgacgat
     2881 acccatctcc gttgccgcgc agggatccag cgcggcatac gtcgcgagat agcgctcggc
     2941 tacgtcgtcg acggcagtgg tgcgcctacc caaggtcgcg cgctgcgaaa gtatcgcact
     3001 tgttcggatc gccggtctga tatcccacag tgaaccaatg ctgccgctgc tcggatgatc
     3061 cgtgtgtcca tgactcgggg ttgacacgtc cggtggcctg cttctggatc cggtcgtcgc
     3121 cgaccgatgc tgcggcggac agcgcgtcgg caatgtcctt gtcgctcaac ggctccaaga
     3181 acggcacgtc agtgccctcc tgcttggttg tcgacgcgta atgcgcccag acgcccgcgt
     3241 agcagtccgc ctgcagttct gtgcggacgc cgtttcccgt ggctccctgt gcgccctgct
     3301 gggcgcggcc gagcgtgccc tgcagattct gcacgtggtg gccgaattcg tgggcgacga
     3361 catactcctg ggcgaacggt ccaccgctgg aaccgaactg ggtgcgc
//
LOCUS       DASYLX010000944         9042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1704431,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000944 DASYLX010000000
VERSION     DASYLX010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704431"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VGP27_22050"
     CDS             <1..388
                     /locus_tag="VGP27_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854012.1"
                     /translation="EGIKDPVKRLRALLEALSEITAMPARLSRGLSVLHLRLSESRPN
                     DLAYALEPLHSFIVELLGAVAEAGLLREDVPLATQAALLQELLLATSHSAVLAGGRST
                     SVDDLWAFCSAAILRPAAESQGAAVR"
     gene            complement(381..1592)
                     /locus_tag="VGP27_22055"
     CDS             complement(381..1592)
                     /locus_tag="VGP27_22055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854013.1"
                     /translation="MDGVKVLEVAQFTFVPAAGAVLADWGADVIKVEHAETGDAQRGL
                     SALMGMPVGSGSFAPLMEHPNRGKRSIGLALEHPDALEILHELIRDSDVFLTNFLPRA
                     RRRLGIDLDDVRKINPEIIYVRGSGFGAHGPDSERGGYDSTAFWARGGSALGTTPVEL
                     DGLCKMPAAAYGDSIGGMTIAGGIAAALLARQRTGETSVVDVSLLGVGAWAYALPVDF
                     ALLAGSAPPRVPRTNTAPTNPLVGNYRTADGRWLVLAMLQPGRYWPEFCARIDREDLI
                     ADARFATTDLLMSHTSEAAHIVAEVLATRTLADWTTAFSGMEGQWAVAQDPWEVGQDA
                     ALRANGLIAEVVGTDGTTHELVANPVQFDQKPVQLTRAPQFAEHTDEILRTLGKTDEA
                     LMDLKVSGAVT"
     gene            complement(1645..2394)
                     /locus_tag="VGP27_22060"
     CDS             complement(1645..2394)
                     /locus_tag="VGP27_22060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854014.1"
                     /translation="MQGRIAVVAGASMGVGRATAIRLAQDGATVVLLARRKLLLDEVV
                     EVIGENAVPIVTDVTDSHDVNAAFDQIAAEFGRIDVLINSAGASRIRLIEEAADEDIA
                     VTVNTNLVAPIYTTRSAIPLLRAAGGGDIVNISSEITLDDMPMMTLYAATKNGLNGFT
                     KAMSKELRRESIRVTLVVLGAVADTGIHENFGPGDLEKAWPLWEADGYLTRMSGTQRL
                     APATVADVLHDVVTRPREVMMDVVHVRPSSS"
     gene            2579..3577
                     /locus_tag="VGP27_22065"
     CDS             2579..3577
                     /locus_tag="VGP27_22065"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7854015.1"
                     /translation="MVTRVTGKEAKDWAAEHLRGNCSSLYTPFCGLDGDDIDYEALRT
                     LVRHCLVDLDQDGIWLTGGIAEFWSLTTDELKEVARVAVEEARRVKPGALVQVMSSTD
                     TAKQAVELTLNAQELGADICYLLTPYFECSGKPGVLEYLRYVADRTDMPLGFFNSHST
                     GLVLTPEECVELYHEIPALCGLKNGLLDAQHSLAVHKLAPEMVLWEASDEAGIKLGIP
                     HPGALGSALYLYEKPGAPLFSEWRELLVQGDFERADAFAADSGLTAMREASRRYQSHP
                     ARPGMFTHWGAGFKFGASLLGLPVGDHPARPPQTAFPDDLKPPIREAYAASGFIES"
     gene            3593..4711
                     /locus_tag="VGP27_22070"
     CDS             3593..4711
                     /locus_tag="VGP27_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854016.1"
                     /translation="MDVREFRAGVCAWIKECKEDVVPAIEDQSVDAQLSHQRRVQQLL
                     FDAGWMRWGWPTRLGGLGGSPILRAVLGEELTSRALVHTTSWSMHEVLGPAVAEFGRP
                     EVVEDIFPKLLRGEEFWCQGFSEPEAGSDLGSLRTTARPRDGGWVLNGEKLWTSWAHH
                     ATRCIVLARTGGPGSRGISAFLVDMETPGVTASPLKTMADVDEFCSTSFVDVEVPADR
                     LLGEVDNGWRVAQFILSCERGPIFWQRGAWLGHHLGMFVETAVDARSHAAIGEAYQLL
                     WAFRATSKTTQDRIGAGDMPGPEASVDKIMIAAADQAVFDAARDLLRGDLETDDSAVG
                     RYWRREWAYSRAATIYGGTSEVQKDIVASRLLGLPRSA"
     gene            4708..5700
                     /locus_tag="VGP27_22075"
     CDS             4708..5700
                     /locus_tag="VGP27_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854017.1"
                     /translation="MTAAAMIESERDMLSRTLRTLTEQFDGAALSERLNDFGFDELLA
                     EAPREAVSALFGGLGRAGSMCTALQAVLRQPLAGVLSERIADVDVALPWIGSDLVGDG
                     DDDTLAIRGLVIGHTAGAACLAPVAIRGELVWVHLADDATLNSRTVEGLDPELAMTEI
                     RGVTAPADVVVAGGSATAVWAAVCTAGRLALGYQILGAVDQMIDLAVEHARSRVQFGR
                     PIGSFQAVRNRLADAHVARQGAASALESAWGVDDAFLAGLLAKSLAGRAARIAATQCQ
                     QVLAGIGFTAEHPFHRFMARALVLDSVLGSAAELPREIGAHLNSVGTIPRLVDL"
     gene            5697..6509
                     /locus_tag="VGP27_22080"
     CDS             5697..6509
                     /locus_tag="VGP27_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7854018.1"
                     /translation="MSDAESSRVSAHPAVVVRRDGPVACVTLARPERANALDAEITDA
                     LLEAILGLRNDADVRAMVVTGQGSAFSAGGDIAAIRAMRDDRRLRERVLAAHQDLFWV
                     MARLPFPTVAAVNGAAVGAGVTVALLCDLVVMADDAFFSDPRVSLGLLDGAGGLVLWP
                     LLTSLSAAKEHLLLGDRVSGTEAHRLGLANRAVPASGVLEEALALAGRLAALPPQAVR
                     EARRLLNSHLDRAAVILADCARAESDCFDTEEQKVQLEQLSARVAAKTGGTT"
     gene            6509..7258
                     /gene="fabG"
                     /locus_tag="VGP27_22085"
     CDS             6509..7258
                     /gene="fabG"
                     /locus_tag="VGP27_22085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEV7854019.1"
                     /translation="MGAGNRVALVTGGTRGIGRAITERLTSSGVTVAAVYARDAAAAH
                     AVRDDAARCGATVTLHQADVGQPSSCQAVVDEVLEQHGRLDYLVNNAGMVLEQRLSEV
                     SATDWHRQVAVNLSSAFFLTQAALAHMTQERFGRVVNIGSVTALLGSPVQIAYGAAKG
                     GIVGLTRSSARAVARKGVTVNCVIPGSFDTDMSAGLTLTDRDAVTSMIPIGRWGRPEE
                     LAHAVAFLLDDLASYVTGSVVTVDGGMSMGG"
     gene            7258..8895
                     /locus_tag="VGP27_22090"
     CDS             7258..8895
                     /locus_tag="VGP27_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854020.1"
                     /translation="MKRAPVTAADIVAPAVAADRLAEYRRFGYVNDLTMSASVVRAAR
                     QWGSRPAIVEGGHQLTYQELLELVERASSWLVGAGVGPGDVVCWQTPNWWEAHVLGLA
                     VWHVGAVSCPIAPFYREHELRQAIEQVRPAAVVTAETFRGFAHAEAFDDVLAAAGLGD
                     AARVVLRGTRPGWSPFDVVVAQGRRQEFASTGADEPCLILFTSGTTSGAKAAVHSSRT
                     LLAETRQLADAWGLSWEDVAYMAAPLQHITGVLNAMTVPLLVGASAVLAERWEPDVAV
                     ADIMRHRVTYSAGATVFLQELTEAARAAGVHLPLRNFACGGATVPRAVMERSEEQGIP
                     ASRVYGMTELPTVTVMNRDYPFDLRAETDGAIAPGVEVRAVGTDGEPMATGCAGELLV
                     RGPEQMLGYLDPAANRAALDEAGWLSTGDVGIVDRAGFVTITGRLKDVINRGGEKFSA
                     RDIEDLLIAHPAVRDAAVVPGPDARFGEVPVAFVVFDQAGRASADDLSRHLHTGGLAR
                     QKTPVAWHFVDSLPMTPSGKVKKFELVARIDAAEGQG"
BASE COUNT         1424 a         2941 c         3105 g         1572 t
ORIGIN      
        1 cgagggcatc aaggatccgg tcaagcgatt acgggccctt ctggaggcgc tgtccgagat
       61 caccgccatg cctgcccggc tttcgcgcgg gctgagcgtc cttcatctgc ggctcagcga
      121 gtcccgcccc aatgacctcg cgtatgccct ggagcccctg cactccttca tcgtcgaact
      181 gctcggtgcg gtcgccgagg cgggcctgct gagggaggac gtgccgctgg ccacccaggc
      241 tgcgctgctt caggaactgc tgctggcgac gtctcactct gcggtgctgg ccggcggtcg
      301 gtcgaccagc gtcgacgatt tgtgggcctt ctgctcggcc gccatcctgc gcccggccgc
      361 cgaaagccag ggtgccgctg tcaggtgacg gcgccgctga ccttcagatc catgagcgcc
      421 tcgtccgtct tgccgagcgt gcgcaggatc tcgtcggtgt gttcggcgaa ttgcggcgca
      481 cgagtgagtt gcaccggctt ctgatcgaac tgcaccggat tcgcgacgag ttcgtgtgtg
      541 gtgccgtcgg tgccgaccac ctcggcgatc agcccgttgg cgcgtaacgc cgcgtcctgg
      601 ccgacctccc acggatcctg agcgacggcc cactggccct ccatgccgct gaacgcggtg
      661 gtccagtcgg ccaacgtgcg ggtcgccaag acctccgcga cgatgtgagc cgcttcggag
      721 gtatgactca tcagcagatc cgtggtggcg aagcgagcgt cggcgatcag gtcctcgcga
      781 tcaatcctcg cgcagaactc gggccagtac cgaccgggct gaagcatcgc gagcacaagc
      841 catcgaccgt cggcagtgcg gtagttgccc accagcgggt tcgtcggtgc cgtgttggtc
      901 ctcggtaccc ggggtggggc actccccgcc aggagcgcga agtcaaccgg tagcgcgtag
      961 gcccaggccc cgacgccgag cagggaaacg tcgaccaccg aggtctcgcc agtgcgttgc
     1021 cgggcaagca gcgcagccgc gattcctccc gcgatcgtca tcccgccgat cgagtcgccg
     1081 taggccgcgg ccggcatctt gcacaagccg tccaactcca ccggcgtcgt ccccaacgcg
     1141 ctaccgccgc gggcccagaa ggcggtgctg tcataaccgc ccctctcgct atctgggccg
     1201 tgcgcaccga agccggaccc gcggacgtag atgatctccg ggttgatctt gcggacgtcg
     1261 tcgaggtcga tgccgagtcg acggcgggcc cggggcagga agttggtcag gaacacgtcg
     1321 ctgtcgcgaa tcagctcgtg cagtatctcc agcgcgtcgg ggtgctccag tgccaggccg
     1381 atgctgcgct tgcctcggtt ggggtgctcc atcagcggag cgaacgaccc gctgccgacc
     1441 ggcataccca tcagtgcgct caggccacgt tgcgcgtccc ccgtttcggc gtgttcgacc
     1501 ttgatcacat cggcgcccca gtcggcgagg accgcaccgg ctgccggcac gaaggtaaat
     1561 tgcgcgacct ccagcacctt cacgccgtcc atcggtcgga tcacgttgtc tcctctagcg
     1621 tttcagatcg gtgtcggtgc cggttcagct gctcgagggc cggacgtgca ctacgtccat
     1681 catcacttca cgcggtcgag tgacgacgtc gtgcaggaca tcggcgaccg tggccggggc
     1741 gagccgctgg gtgccactca tccgggtcag gtagccgtcg gcctcccaga gtggccaggc
     1801 tttttcgagg tcgccggggc cgaagttctc atgaattccg gtatcggcga cggcaccgag
     1861 aacgaccaag gtcacgcgga tgctctctcg ccgcaactcc ttgctcatcg ccttggtaaa
     1921 gccgttgagt ccattcttgg tggcggcgta cagggtcatc atcggcatgt cgtccagcgt
     1981 gatctctgag gagatgttca cgatgtcgcc tccgcctgcc gcgcgcagca acggaatcgc
     2041 cgagcgtgtg gtgtagatcg gtgccaccag gttggtgttg accgtgacgg cgatgtcctc
     2101 gtcggcggct tcttcgatga gccggatgcg gctggcgcct gcggagttga tcaggacatc
     2161 gatacggccg aactccgccg cgatttgatc gaacgcggcg ttcacatcgt ggctgtcggt
     2221 gacgtcggtg acgatgggca cggcgttctc cccgatgact tcgaccacct cgtcgaggag
     2281 gagtttgcgg cgcgcgagca gtaccactgt cgcgccgtcc tgcgcgaggc ggatagcggt
     2341 ggcccggccg actcccatcg atgcgcctgc cacgacggcg atgcggcctt gcatgccttt
     2401 caacatcgca ctccctgtgt tcgtgtctgc agagctccaa aaatgtgcgg accaccctgc
     2461 attcccagtg cacctgttcc ttgcacggaa tgcagataac gttctaccat ggcgcgtacg
     2521 tgatagtcat cacctggccc gagaactcgt gccggcacag ttccgaggga ggtcgattgt
     2581 ggtcacgcgt gtcaccggca aagaggccaa ggactgggcg gcagaacatc tgcgcggcaa
     2641 ctgcagttcg ctgtacaccc cgttctgcgg cctcgacggc gacgacatcg attacgaggc
     2701 attacggacg ttggtccggc actgcctggt cgacctcgac caggacggca tctggctgac
     2761 cggcggcatc gccgaattct ggtcattgac caccgacgaa ctgaaagaag tagcccgggt
     2821 cgccgtcgaa gaggcgcggc gagtcaaacc cggcgccctg gtgcaggtga tgtcgagcac
     2881 cgacaccgcc aagcaggccg tcgagctcac gctgaacgcc caggagctgg gcgccgacat
     2941 ctgctacctc ctcacgccct acttcgagtg ctccggtaaa cccggcgtgc tggagtatct
     3001 gcggtacgtc gccgaccgaa cagacatgcc cttgggcttc ttcaactctc attcgacggg
     3061 cctggtgctg accccggagg aatgcgtcga gctctatcac gagatccctg cgctgtgcgg
     3121 cctgaagaac gggctgctcg acgcgcagca cagcctggcg gtgcacaagc tggccccgga
     3181 gatggtgctg tgggaagcgt ccgacgaggc ggggatcaag ctggggatac cgcacccggg
     3241 agcactgggt tcggcgctgt acctctacga gaagccgggg gcgccgctgt tcagtgaatg
     3301 gcgagaactg ctggtgcagg gagacttcga gcgcgccgat gccttcgccg ccgattctgg
     3361 tttgaccgcc atgcgggaag cgagcaggcg ctaccagtcc catccggccc gacccgggat
     3421 gtttacgcac tggggtgccg gattcaagtt cggcgcctca ttgcttgggc ttcccgttgg
     3481 cgaccacccc gcgcggccgc cgcagaccgc cttccccgac gacctgaagc cgccgattcg
     3541 cgaggcgtac gccgcatccg gattcatcga gtcctgagaa cggagggctg tcgtggacgt
     3601 gcgcgaattt cgcgcggggg tgtgcgcgtg gatcaaagag tgcaaagagg acgtggtccc
     3661 ggcgatcgag gaccagagtg tcgacgcgca gttgagccac cagcgccggg tgcagcagct
     3721 gctgttcgat gccgggtgga tgcgttgggg atggccgaca cggctcggtg gtttgggcgg
     3781 ctcgcccatc ctgcgggccg tcctcggcga ggagctcacc agccgggcac tggtgcacac
     3841 gacgtcctgg tcgatgcacg aggtgctcgg accggcggtg gccgagttcg gtcgacccga
     3901 agtcgttgag gacatctttc ccaaactgct gcggggcgag gagttttggt gccaggggtt
     3961 ctccgagcct gaagcgggaa gcgatctggg gtcgctgcgc acgaccgctc gtccgcgcga
     4021 cggcggttgg gtgctcaatg gcgagaagct gtggaccagc tgggcccacc acgccacccg
     4081 ttgcatcgtc ttggcccgca ccggcggccc ggggtcccgc ggcatcagtg ccttcctggt
     4141 ggacatggag acgccggggg tcaccgccag tccgctgaag accatggccg acgtcgatga
     4201 attctgctcg acgtcattcg ttgacgtcga agttcccgcg gatcggctgc tcggcgaagt
     4261 cgataacggt tggcgggtag cgcagttcat cctgtcctgt gaacgaggcc ccattttctg
     4321 gcagcgcggt gcgtggctgg gccatcacct cggaatgttc gtcgaaaccg cagttgacgc
     4381 tcggtcgcac gccgccatcg gcgaggccta tcaactgttg tgggcattcc gcgccacctc
     4441 gaaaaccacc caggacagga tcggcgcggg tgacatgccc ggacccgaag cctccgtcga
     4501 caagatcatg attgccgccg ccgaccaagc cgtgttcgat gcggcccgcg atctgctccg
     4561 tggcgatctg gagacggacg actccgccgt ggggcggtac tggcgtcgcg agtgggctta
     4621 ctcgcgcgcc gcaaccatct acggcggcac cagtgaagtt cagaaggaca tcgtggcatc
     4681 gcgcctgctc ggattgccga gaagcgcgtg accgcggctg cgatgatcga gtccgagcga
     4741 gacatgctct cgcggacgct gcggacactg accgagcagt tcgacggcgc agcactctcc
     4801 gagaggctca acgacttcgg gttcgacgaa ctactcgccg aggcaccccg cgaggcggtg
     4861 tcggcattgt tcggcggtct gggccgggcg ggttcgatgt gtacggcgct gcaggccgtg
     4921 ctgcggcaac cactggccgg cgttctgtcc gaaagaattg cggatgtcga tgtcgcattg
     4981 ccctggatcg gcagcgacct cgtcggcgac ggcgatgacg acacactggc gatacgcggc
     5041 ctcgtcatcg gacacacagc cggtgcggca tgtttggcgc cggtggcgat acgcggcgaa
     5101 ctcgtgtggg tacatctggc cgacgacgcc accttgaaca gccggaccgt cgaaggactc
     5161 gatccggagc tggcgatgac cgaaatcagg ggggtgactg caccggcgga tgtggtggtg
     5221 gccggcgggt ccgcgacggc ggtctgggcg gcggtgtgta ctgcgggaag gttggcgctg
     5281 ggctatcaga tcctcggggc tgttgatcag atgatcgacc tcgccgtcga gcacgcgcgc
     5341 agccgagtgc agttcggcag gcccataggc tcgtttcagg cggtgcggaa tcgactcgcc
     5401 gacgcacacg tcgcccgtca gggcgccgcc tctgccctcg agtcggcgtg gggcgtcgat
     5461 gacgccttcc tggcaggtct gctggccaag tcgctggcgg ggagggcggc gcgaatcgcc
     5521 gcgacccaat gtcagcaggt gctggccggc atcggcttca ccgccgagca tccgttccac
     5581 cgcttcatgg cccgcgcact cgtgctcgat tcggtattgg gttcagcggc cgaactgccc
     5641 agagaaattg gcgctcacct gaattcggta gggacgatcc cccggctggt cgacctgtga
     5701 gcgatgcgga atcgtcccgg gtatctgccc acccggccgt cgtggtccgc cgggacggcc
     5761 cggtggcatg cgtgaccctg gcccggcccg aacgagccaa cgccctggac gccgagatca
     5821 ccgatgccct tctcgaagcc atcctcggct tgcggaacga cgccgacgtc cgtgcgatgg
     5881 tggtcaccgg ccaaggcagc gccttcagtg ctggtgggga catcgcagcc atccgcgcca
     5941 tgcgcgatga ccgtcggctg cgggagcggg tgctggctgc gcaccaggac ctgttctggg
     6001 tgatggcgcg actgccgttc ccgaccgtgg ccgctgtcaa cggcgccgcg gtcggcgccg
     6061 gcgtcacggt ggcattgttg tgtgacctgg tggtgatggc cgacgacgct ttcttcagcg
     6121 accctcgagt gtcgctcggc ctgctcgacg gggccggagg tttggtgctc tggccactgt
     6181 tgaccagtct gtcggccgcg aaggaacacc tcctccttgg cgaccgggtc agcggcacgg
     6241 aagcccatcg cctcggtctg gccaaccggg cggtgcctgc ctcaggcgtg ctcgaggagg
     6301 cgttggcgct ggccggccgc ctcgctgcgc tcccgccgca ggcggtaagg gaggcccgtc
     6361 ggctgctgaa ctcccacctc gaccgggccg cggtgatcct ggcggactgc gcgcgcgccg
     6421 agagcgattg cttcgacacc gaagagcaaa aggtgcagtt ggagcaactg agcgcgcggg
     6481 tggcggccaa gaccggcggc accacctagt gggcgccggc aaccgggtgg cgctcgtgac
     6541 cggtggaact cgggggatcg gtcgtgccat caccgagcga ctgaccagct cgggtgtcac
     6601 ggtggccgcg gtgtacgccc gcgacgctgc cgcggcacac gcggtccgcg acgatgcagc
     6661 gcggtgcggt gccaccgtca cgctgcatca ggccgatgtc gggcagccgt cgtcatgcca
     6721 ggcggtggtc gacgaagtcc tcgaacagca tgggcggctg gattatctgg tcaacaatgc
     6781 cgggatggtg ctcgagcaac gcctcagcga ggtcagtgcc acggactggc accggcaggt
     6841 ggcggtgaac ctgtcttcgg cgttcttcct gacccaggcc gcgttggcgc atatgacgca
     6901 agagcgattc gggcgggtcg tcaacatcgg ttcggtcacg gcactgttgg gcagtcctgt
     6961 ccagatcgct tacggcgccg caaaaggcgg catcgtcggc ctgacccgct ctagtgcgcg
     7021 cgccgtggca cgcaagggcg tcaccgtgaa ctgcgtcatc cccggcagtt tcgacaccga
     7081 catgtccgcc gggctgacgt tgaccgaccg ggacgcggtg acgtcgatga ttccgattgg
     7141 acgctggggc cgacccgagg agctggcgca tgccgtcgcg ttcctgctcg acgaccttgc
     7201 gtcctacgtc accggatccg tcgtgaccgt cgacggcgga atgagcatgg gcggttgatg
     7261 aagcgagccc cagtcaccgc ggccgacatc gtcgcacccg ccgttgccgc agaccgcctc
     7321 gcggaatatc gacgattcgg gtacgtcaac gacctcacga tgtccgcgtc ggtcgtgcgc
     7381 gccgcgcggc agtggggctc acggccggcc atcgtcgaag gtggccatca gctgacctac
     7441 caggagcttc tcgagctggt cgaacgcgcc agttcgtggc tggtcggagc gggcgtcgga
     7501 cccggagatg tcgtctgctg gcagacgccc aactggtggg aggcccacgt gttggggctg
     7561 gcggtctggc atgtcggggc ggtgagctgc ccgatcgccc cgttctaccg ggaacacgaa
     7621 ctgcgccagg ccatcgaaca ggttcggcca gcagctgtgg tgacggcaga gacgttccgc
     7681 ggctttgcgc acgccgaagc tttcgacgat gtgctcgccg ccgccggtct gggcgatgcg
     7741 gcccgcgtgg tgctgcgcgg aacccggccg ggctggtcgc ccttcgacgt cgtcgtcgca
     7801 cagggccgtc gccaggagtt cgcctccacg ggggccgacg aaccctgtct gatcctcttc
     7861 acgtcgggga ccacctcggg cgccaaggcc gcggtgcact cctcgcgcac attgctggcc
     7921 gaaacccgcc agctcgccga tgcgtggggc ctgtcctggg aggacgtcgc ctacatggcg
     7981 gccccgctgc aacacatcac gggcgtgctg aacgcgatga ccgtcccgct actggtcggc
     8041 gcctcggcgg tgctggccga acgttgggaa cccgacgtcg cagtggccga tatcatgcgc
     8101 caccgggtga cctactcggc tggcgcgacc gtgtttctgc aggagctgac ggaagccgcc
     8161 cgcgccgccg gagtgcacct gccgctgcga aacttcgcct gcggaggggc gacggtaccg
     8221 cgcgcagtca tggaacgcag tgaggagcaa gggattcccg cgtcgcgcgt atacggcatg
     8281 acagagcttc caactgtgac tgtgatgaac cgcgactatc ctttcgacct gcgcgccgaa
     8341 accgacggcg cgatcgcacc gggggtcgag gtccgggccg tcgggacgga cggagagcca
     8401 atggcgacgg gatgtgccgg cgagctgctg gtgcgcggac ccgagcagat gctgggctac
     8461 ctcgaccccg cggccaaccg ggccgcgctc gacgaagcgg gatggttgtc cacaggggac
     8521 gtcggcatcg tcgatcgcgc cggcttcgtc accatcaccg gacgcctgaa ggacgtcatc
     8581 aaccgcggcg gtgaaaagtt ctctgcgcgt gacatcgaag acctgctgat cgcacacccc
     8641 gcggtgcgcg acgccgccgt ggtgcccgga cccgacgcga gattcggtga ggttcccgtc
     8701 gctttcgtgg tgttcgatca ggcgggccga gcgtcggccg acgacctgtc ccggcatttg
     8761 cacaccggcg gtctggcgcg ccagaagacc cctgtcgcat ggcatttcgt ggactcattg
     8821 ccgatgacgc cgtcgggcaa agtcaagaag ttcgaactcg tcgcacgaat cgatgccgca
     8881 gaagggcaag gatgaatctc gaccaatttc gcgccatgat gactctcggt gatgacgccg
     8941 agggtcgcac ggtgctgccg gtgccggacc cgaacgtcga gcggattttc ggcggccaga
     9001 tcatggcgca gctcattgcc gccgctgcgc cgcccggatc ga
//
LOCUS       DASYLX010000945         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_279874,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000945 DASYLX010000000
VERSION     DASYLX010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279874"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..750
                     /locus_tag="VGP27_22095"
     CDS             250..750
                     /locus_tag="VGP27_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEV7854021.1"
                     /translation="MTTTAIERPFRGVRLRYDSAKSYDELVTALLADIGDEPVPINDI
                     AAGSDSWESYEREVASHAGPSGFMLFSLIDHGSWLEKAGIGRKVLRVLLGNPLIAITM
                     LRHDATAGLFAPVELLITDEEDGTSALSYVVPSSLMVVEPNEPLRTAALKLDAKLAAL
                     AASVTA"
     gene            complement(747..1046)
                     /locus_tag="VGP27_22100"
     CDS             complement(747..1046)
                     /locus_tag="VGP27_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7854022.1"
                     /translation="MATPLSIATDRRDDGTFVVTATGEIDMSNVDSFAGALSTVKSDG
                     EATTVDLSGVEYLDSGAINVLFAHAEGMRLIANPILLPVLTISGLADVATVERAD"
     gene            complement(1046..>4003)
                     /locus_tag="VGP27_22105"
     CDS             complement(1046..>4003)
                     /locus_tag="VGP27_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV7854023.1"
                     /translation="VNTLLDFSRIEAGRTQAHYEPVDLAAVTAELASVFRSAVDRAGL
                     RFTVDCPALDEPVYVDRDMWEKVVLNLLSNALKFTFDGSISVRVYREGSDAVVAVSDT
                     GIGVAAEEMPRLFERFHRIESAHARSNEGSGIGLALVQELVNLHGGTITAESTEGAGT
                     TFEIRLRFGADHLPADAVTPTTGTRSGSGLAEPYVQEALRWIPGDAAAENTQDVVEPL
                     PDTTVVHAPEAATTAHVLIADDNADMREYLERLLTGAGYRVEAVTDGRHALEAVRADM
                     PDLVVSDVMMPRLDGLELVAALRSDPRTAAVPVLLLSARAGQEASIEGLQAGADDYLV
                     KPFAAAELLARMRGIIELARLRNHHARWRTALVDSLQEAFFVTDEQGAVIEINAAFSD
                     IMGYGSDPLPFRPPFPWWPSADADPVARRQIEAAFAQLVADEHGTFTVPVNHRDGHRL
                     WVTVNFNHADDPDTGRRLTVGTFRDVTAEHYTVQRESALASLNQHLAQADTLDDAVRA
                     AAEELRNVWRAHRVSAVTLSPDSAETGAPQFMCVGEPTQWSDLPPRTQQMVRSLADRD
                     LLTPDTSQPGMAGVALQHPRGVLVLHIELAERRLFTSEDNTLLTVLAGRLGQGLQRVH
                     QLDQQRETALALQNAILGPANLPTGFAVRYQPATRPLQVGGDWYDVVDLDDGRIALIV
                     GDCVGHGLAAATVMGQLRSACRALLLENASPSAALAAMDQFAARLPGARCTTAFCAVL
                     SPDTGELVYSSAGHPPPIMVLADGTTQFLDGARATPLGLSFDRSRPEQRETLPPRATL
                     MLYTDGLVERRRESLDTGIARAVELVAENRASALDDLAKQVMTRLAPGGGYQDDVALL
                     LYRQPAPLEVDFPAEASHLAPTRAALKGWLTRAGMDDDQTLKVLIATGEALANAIEHG
                     HRLLTEGNISLRAIALADRVHVTVVDAGSWKPPVAAAHRGRGIALMRALMQDVTIQQR
                     ATGTTVHMHTRIA"
BASE COUNT          652 a         1306 c         1365 g          680 t
ORIGIN      
        1 ggtcgagcac ggccagcttc ccgcgcagat gtcggccctc accgacgtcg ccaacacgtt
       61 ggtgtccctg gtcgtcacgg ctcatgcgaa tccgagcgtg gtgcccgaga tcgtgcagtt
      121 gtcgccgcgc accaggccga ctacctgagt tcagtccaca aaacccagga atacacaccg
      181 ccgaacgttc gttacccgtc gaagatctgc agaattttcg atcggtacgc aacgttcgcg
      241 ggaggtttga tgaccaccac tgccattgag aggccgtttc gaggggtgcg gctgcgatac
      301 gacagcgcca agagctacga cgaactggtc accgcgctgc tcgccgacat cggtgacgaa
      361 cccgtgccga tcaacgacat cgcggctgga tccgacagct gggagtctta cgagcgagag
      421 gtggcttccc acgccggacc cagcggcttc atgctcttct ccttgataga ccacggctca
      481 tggctcgaga aagcgggcat cggcaggaag gtgctgcgtg tcctgctcgg caacccgctg
      541 atcgcgatca cgatgctgcg acacgatgcg accgcgggac tctttgcgcc cgttgaactc
      601 ctgatcaccg acgaagagga cggcacgagc gccctgtcct atgtggtgcc gtcgtcgctg
      661 atggtcgtcg aacccaacga accgcttcgc actgcggcac tgaaactgga cgccaaactc
      721 gcggcgctgg ccgcgtcggt gacggcctag tcggcgcgtt cgacagttgc cacgtccgcg
      781 agaccactga tcgtcagcac cggcaacagg attgggttgg cgatcaatcg catgccctcc
      841 gcgtgggcga acaacacgtt gatggcgccg ctgtccagat actccacgcc gctgaggtcg
      901 accgtcgtcg cctcgccgtc ggacttcacc gtcgacaggg ctccggcgaa cgaatcgacg
      961 ttgctcatat cgatctcccc cgtcgcggtc accacgaagg tcccgtcgtc gcggcggtcg
     1021 gtcgcgatgc tcagcggcgt ggccatcacg cgatccgggt gtgcatgtgg accgtggtgc
     1081 ccgtggcgcg ctgctggatg gtgacgtcct gcattagggc gcgcatgagc gcgatgcccc
     1141 ggccgcggtg ggcggcggcg accggcggtt tccacgagcc ggcgtcgacg acggtgacat
     1201 gcacccgatc ggcgagtgcg atcgcacgca ggctgatgtt gccctcggtg agcaggcggt
     1261 gcccgtgctc gatcgcgttg gcgagcgcct ccccggtggc gatcagcacc ttcagcgttt
     1321 gatcgtcatc catgccggcc cgggtcagcc atcctttgag cgcagctctg gtgggggcga
     1381 gatggcttgc ttcggcggga aaatccacct cgagaggggc gggctggcgg tacagcagca
     1441 gggcgacgtc gtcctgatag ccaccgccgg gcgccagccg cgtcatcacc tgcttggcga
     1501 ggtcgtccag cgcgcttgcg cgattctccg ctacgagttc cacggcgcgg gcgataccgg
     1561 tgtccagcga ttccctgcgc cgttcgacca gaccgtcggt gtagagcatc agcgtcgccc
     1621 gcggcggcag ggtttcgcgt tgttcgggcc gagaccggtc gaaggacagc ccgagcggcg
     1681 tggcccgcgc accgtcgaga aattgcgtgg ttccgtcggc cagcaccatg atcgggggcg
     1741 gatggcctgc gctggagtac accagctcac cggtgtcggg gctgagcacc gcgcagaatg
     1801 cggtggtgca tcgcgcgccc ggcagccgtg cggcgaactg gtccatcgcc gccagtgcgg
     1861 cgctcgggct ggcattctcc aaaagcaatg cgcgacaagc acttcgcagc tgtcccatga
     1921 cagtcgccgc ggccaggccg tgcccgacgc agtcgccgac gatgagcgcg atgcggccgt
     1981 cgtcgaggtc gacgacgtcg taccagtcac cgccgacctg aagcggacga gtggctggtt
     2041 ggtagcgcac ggcgaatccg gtgggcaggt tcgccggacc gaggattgcg ttctgcagcg
     2101 ccagtgccgt ctcccgctgc tggtcgagtt gatgcacccg ctgcagtccc tggccgagtc
     2161 ggcccgcgag caccgtgagc agcgtgttgt cttcggacgt gaagagtcgt cgttcggcca
     2221 actcgatgtg cagcacgagg acaccgcggg gatgttgcag cgcgacccct gccattccag
     2281 gctgcgatgt gtcgggtgtg agcagatcac gatcggccag cgacctgacc atctgttgcg
     2341 tacgcggcgg cagatcagac cactgcgtcg gctcaccgac gcacatgaac tgcggcgcac
     2401 cggtttcggc gctgtcgggc gacaaggtga cggccgagac gcggtgggca cgccacacgt
     2461 ttcgcagttc ttcggctgcc gcacgtacag cgtcgtcgag ggtgtcggcc tgggcgaggt
     2521 gctggttgag cgaggccagc gcgctctcgc gttgcacggt gtagtgctcg gcggtgacgt
     2581 cgcggaacgt gccgaccgtg aggcggcgtc cggtatccgg atcgtcggcg tggttgaagt
     2641 tgaccgtcac ccagagccgg tgtccgtcgc ggtggttgac cggaaccgtg aatgtgccgt
     2701 gctcgtcggc caccagttgg gcgaacgccg cttcgatctg cctgcgcgcc acgggatccg
     2761 cgtcggccga cggccaccac ggaaagggag gccggaaagg cagcggatcg gagccgtacc
     2821 ccatgatgtc gctgaacgcg gcgttgatct cgatcaccgc gccctgctca tcagtgacga
     2881 agaatgcctc ttgcaacgag tcgacgagag ccgtgcgcca acgtgcgtgg tgattgcgca
     2941 accgcgccag ttcgatgatc ccacgcatgc gcgccagcag ttcggcggct gcgaacggct
     3001 tgacgaggta gtcgtcggcg ccggcctgca gcccttctat cgacgcctcc tgcccggcgc
     3061 gcgcggacag cagcagcacg ggcacggccg cggtgcgcgg atcggaccgc agcgcggcca
     3121 ccagttccag cccgtccagc cggggcatca tcacgtcgct gacgaccagg tcgggcatgt
     3181 cggcgcggac tgcctccaac gcatgcctgc cgtcggtgac cgcttcgacg cggtagcctg
     3241 cgccggtcag caggcgttcc aggtattcgc gcatatcggc gttgtcgtcg gcgatcagca
     3301 cgtgagccgt cgtcgcggcc tccggggcgt gaacgacggt ggtgtctgga agcggttcga
     3361 ccacgtcttg ggtgttctcg gcggcggcat cgccgggtat ccaccgcagc gcctcctgga
     3421 cgtacggttc ggcgagcccg gaacccgacc gcgttcctgt ggtcggggtg acggcgtcgg
     3481 ccggcaggtg gtcggcgccg aaacgcagac ggatctcaaa tgtggtgcct gcaccctcgg
     3541 tgctctccgc cgtgatggtg ccgccgtgca ggttgaccaa ctcctgcacc aacgccagac
     3601 cgataccgct gccttcattg gaacgcgcgt gcgcggactc gatgcgatga aatcgttcga
     3661 acagccgagg catctcctcg gcggccacac cgattccggt gtcggacacg gcgacgacgg
     3721 cgtcgctgcc ctcgcgatac acccgcacgg agatggaccc gtcgaaggtg aacttcagcg
     3781 cgttggacaa caggttgagg acaacctttt cccacatgtc gcgatcgacg tagacgggtt
     3841 cgtcgagcgc aggacagtcg acggtgaacc gcagcccggc gcgatcgaca gcagaccgga
     3901 agacgctggc caactcggcg gtgacggccg cgagatccac cggctcgtag tgcgcctggg
     3961 tgcggcccgc ctcgatgcgg gagaaatcca gcaacgtgtt cac
//
LOCUS       DASYLX010000946         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2251956,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000946 DASYLX010000000
VERSION     DASYLX010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251956"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGP27_22110"
     CDS             complement(<1..576)
                     /locus_tag="VGP27_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854024.1"
                     /translation="MANYPSDGVDRRRQRRSSTPSANRYLPPLEERPSSRGEPPPGSG
                     EGTGRRVNQGPRSAAQRGREVGSKMYGLFQRAATADGADKSGLTALTYPVMANFAVDA
                     AMAVALANTLFFAAASGESKSKVALYLLITIAPFAVIAPLIGPALDRLQHGRRLALAT
                     SFALRTALVVVLIANYDGATGSFPSWVLYPCA"
     gene            698..1687
                     /locus_tag="VGP27_22115"
     CDS             698..1687
                     /locus_tag="VGP27_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEV7854025.1"
                     /translation="MTAPCSRPVWLHARRSGTIKRVDSVTESTEDISATPTIERSPDL
                     EAVLMGAVDQARAAIAEHSGDTVGDYLGATFDDSTAATHRFLANLPGYQGWQWAVVVA
                     AHPGADHATISEVVLVPGPTALLAPKWVPWQERLQPGDLGPGDLVSAPTEDPRLVPGF
                     AATGDPAIDDVAYELGLGRRRLLSQLGRVDAAQRWHDGDFGPASTMAKSTRRVCRDCA
                     FYLPLSGALGLMFGACANELSADGHVVDAEYGCGAHSDTPAPAGTGSPLFDPYDDGML
                     DVVEPPVPSEQAEPEAAIGDEPEAATGDEPEAAVGDEQPIGDQSSADADQDPT"
     gene            complement(1701..2066)
                     /locus_tag="VGP27_22120"
     CDS             complement(1701..2066)
                     /locus_tag="VGP27_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV7854026.1"
                     /translation="MSDASKKIDARIDELGDWRGDTLARIRALIKRAEPDVVEEWKWR
                     GVPVWYRDGMICTGETYKQYVKVTFAKGASLQDPSGLFNASLDGNTRRAIDIREGDKI
                     DDKAFTALIQAAADLNSRR"
     gene            complement(2110..2520)
                     /locus_tag="VGP27_22125"
     CDS             complement(2110..2520)
                     /locus_tag="VGP27_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854027.1"
                     /translation="MDITIHSSFLPLDDPEESLTFYRDTLGFEVRLDVGKDKMRWITV
                     GPPNQPDTSIVLYPPAATPGMTDEERRVVTEMMAKGTFGTLLLATKDLDDTFERVQSG
                     DVEVVQEPTEQPYGVRDCALRDPAGNMVRIQELR"
     gene            complement(2526..>2840)
                     /locus_tag="VGP27_22130"
     CDS             complement(2526..>2840)
                     /locus_tag="VGP27_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7854028.1"
                     /translation="GHLSRQFKSAYGESPYSYLMTRRIERAMALLRRGDTSVTDVCFA
                     VGCSSLGTFSTRFTELVGVSPSAYREQTAGVTEGIPSCVEKQVTRPIRNREAPAARLH
                     LA"
BASE COUNT          441 a          944 c          994 g          461 t
ORIGIN      
        1 cgcacatggg tacagcaccc acgacgggaa gctgcccgtc gcaccgtcgt agttcgcgat
       61 caacaccacg accagagcgg tgcgcagcgc gaacgatgtg gccagggcga gtcggcggcc
      121 gtgctggaga cggtccagcg ccggaccgat cagcggggcg atgacggcga aaggggcgat
      181 cgtgatgagc aggtacagcg caaccttgct cttgctctcg ccggacgccg ccgcgaagaa
      241 cagggtgttg gccagtgcga cggccatcgc ggcgtcgacg gcgaagttgg ccatcacggg
      301 gtaggtgagc gcggtcagcc cggacttgtc ggcgccgtcg gctgtggcgg cgcgctgaaa
      361 caggccgtac atcttcgagc ccacctcgcg gccgcgctgg gccgccgagc ggggcccctg
      421 gttgacgcgg cgccccgtgc cttcgccgct gccgggaggc ggctcgccgc ggctggacgg
      481 ccgctcctcg agcggcggca ggtagcggtt ggcgctcggc gtggaggagc ggcgctgccg
      541 acgacgatcc acaccgtcgc tgggatagtt cgccattccc gggtgttcgt cggcggcggc
      601 gggcggtcgc ggcgggtagt agcggggatc gcgctgggca tcacggcctg gggggtcacg
      661 ccgcaatccg gtcacacaaa gattgtcccc catcgcgatg acggcgccgt gcagccgacc
      721 ggtgtggctg cacgcgcgcc ggtcaggcac tattaagcgc gtggacagcg tgacggaatc
      781 caccgaggac atctcggcga cgccgacgat cgaacggtcg ccggacctgg aggcggtgct
      841 gatgggcgcc gtcgaccagg cacgcgccgc gatcgccgag cacagcggcg acaccgtcgg
      901 cgactatctg ggcgcgacgt tcgacgactc cactgcggcc acccaccgtt tcctggccaa
      961 cctgcccggc tatcagggct ggcagtgggc tgtggtggtg gccgcgcatc cgggcgccga
     1021 ccacgcgacg atcagcgaag tggtgttggt gccggggccg acggcgctgc tggcgccgaa
     1081 gtgggtgccc tggcaggagc ggctgcagcc cggcgacctc ggtcccggtg acctggtctc
     1141 cgcgcccacc gaggatcccc gcctggtgcc gggtttcgcc gcaaccggtg atccggcgat
     1201 cgacgacgtc gcctacgagc tgggtttggg ccgacgtcgt ctgctcagcc agctgggccg
     1261 ggtagacgcc gcccagcggt ggcacgacgg cgacttcggc ccagcgtcca cgatggccaa
     1321 gtccacccgg cgggtgtgcc gggattgtgc cttctacctt ccgctgtccg gagcgctcgg
     1381 gctgatgttc ggcgcatgcg ccaacgagtt gtccgccgac ggccatgtgg tcgacgccga
     1441 atacggttgt ggcgcccatt cggatacccc ggcgcccgcc ggaaccggat cgccgctctt
     1501 cgacccctac gacgacggca tgctcgacgt cgtcgagccg ccggtgccca gcgagcaggc
     1561 cgagccggaa gcggcgatcg gagacgagcc agaagccgcg accggagacg agccagaagc
     1621 cgcggtcgga gacgagcagc cgatcggcga tcagtccagc gcagacgccg atcaggatcc
     1681 gacgtagccg gctgagcgct tcaccgtcgg ctgttcagat cggccgcggc ctggatgaga
     1741 gccgtgaagg ccttgtcgtc gatcttgtcg ccctcgcgaa tgtcgatggc gcggcgggtg
     1801 tttccgtcca ggctggcgtt gaacaggccc gacgggtctt gcagtgaggc gcccttggcg
     1861 aacgtcacct tgacgtactg tttgtatgtc tcaccggtgc agatcattcc gtcgcggtac
     1921 cacaccggca caccgcgcca cttccattcc tcgacgacgt cgggttcggc ccgcttgatg
     1981 agcgcgcgta tccgggccag cgtgtcgccc cgccaatcgc cgagttcatc gatccgcgca
     2041 tcgatcttct tcgaggcgtc gctcatgccc cggctccccc tcggctccca ctaatccttc
     2101 ggtcgctcat cagcgcagtt cctgaatgcg gaccatgttg cccgccggat cgcgcagggc
     2161 gcagtctcga acgccgtacg gctgctcggt cggctcctga acgacctcga catcgcccga
     2221 ctggacccgt tcgaaggtgt cgtcgagatc cttggtggcc aacagcaggg tgccgaatgt
     2281 ccctttggcc atcatctcgg tgacgacgcg gcgctcttcg tcggtcatgc cgggagtggc
     2341 ggcaggcggg tacaacacga tggatgtatc gggctggttg ggtggaccga ccgtgatcca
     2401 gcgcatcttg tccttgccga cgtcgaggcg gacctcgaag ccgagggtgt cgcggtagaa
     2461 cgtcagcgac tcctccgggt cgtcgagggg caggaagctc gagtgaatgg tgatatccat
     2521 ggcgatcacg ctaagtgcag cctggccgcg ggcgcttctc gattcctgat cggtctggtc
     2581 acctgcttct ccacgcacga cggaattccc tctgtcacgc cggcggtctg ttcccgatag
     2641 gcactcggcg atacgccgac caactcggtg aaccgggtgc tgaaggtgcc gagcgacgag
     2701 cagccaaccg cgaagcagac gtcggtgacc gacgtgtcac cgcgccgcaa cagagccatc
     2761 gcacgttcga tgcgccgcgt catcagatag gagtacggcg actcgccgta ggccgacttg
     2821 aactggcggg acaaatggcc
//
LOCUS       DASYLX010000947         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1704536,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000947 DASYLX010000000
VERSION     DASYLX010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704536"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..921)
                     /locus_tag="VGP27_22135"
     CDS             complement(133..921)
                     /locus_tag="VGP27_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV7854029.1"
                     /translation="MEIIPARLKEPMYRLYEMRLRQGLTASKSELPRHIAVLCDGNRR
                     WARDAGHDDVSYGYRAGATKIAEMMRWCQEAGIEMATVYLLSTENLQREPEELSSLID
                     IITDVVEEICAPANRWSVRTVGDLELLGEEPARRLRDAVESTNGHGGFHVNVAVGYGG
                     RQEIVGAVRALLGKEIANGANGEQLIEAVTADAISENLYTSGQPDPDLVIRTSGEQRL
                     SGFLLWQSAYSEMWFTEAYWPEFRRVDFLRALRDYTARHRRYGR"
     gene            976..1722
                     /locus_tag="VGP27_22140"
     CDS             976..1722
                     /locus_tag="VGP27_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV7854030.1"
                     /translation="MTASIDTEEPDRTQRAGDGAEDFPEAVVDRVAHAIGKPRARGWI
                     HVYAAVVALIAGAALVSVSWAMAGTRAGLATLIYTLTIVAMFAVSGAYHRINWKSETL
                     RKWMKRADHSMIFVFIAGSYTPFALLALPSESGMVLLWIVWSGALAGVLLKMFWPSAP
                     RWVGVPLYLLLGWVAAWFVGPILDGAGVAALVLLIVGGALYSVGGVLYGLKWPDPWPA
                     TFGHHEFFHACTAVAAICHYVAMWFAVFRG"
     gene            complement(1712..2104)
                     /locus_tag="VGP27_22145"
     CDS             complement(1712..2104)
                     /locus_tag="VGP27_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854031.1"
                     /translation="MSTPEDNAKTVKRYLELAAQGNVEAIVELYADDGTVEDPVGSEV
                     HIGRQAIHGFYSQIPDTDKSTELDSLRTLGNEAAFSWALIIDLGEGGVRIEIISTMTF
                     NEDGKIASMKAYWSPENIKPMAAGEVSP"
     gene            complement(2115..>3634)
                     /locus_tag="VGP27_22150"
     CDS             complement(2115..>3634)
                     /locus_tag="VGP27_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854032.1"
                     /translation="GLPGGGPEVQPALCDQAVAAVLDDEDVERGGFSTGSQGAPKFPP
                     SALLEALLRSHERTGEPAPLAAVERTAAAMARGGIYDQLAGGFARYSVDDSWVVPHFE
                     KMLYDNALLLRAYAHLARRTGNQLARRVVRETARFMIDELGVDGMFVSSLDADAAGVE
                     GLTYAWTPGQLTEVLGADDGAWAASLFGVTEAGTFEHGSSVLQLPADPDDPGRFDRVR
                     TALLAARSIRPQPGRDDKVITAWNGWAITALAEASVALDEPEFLDAATRCARVLLGLH
                     IADGRLRRASLAAHVGDSAAILEDHATLATGLLTLHQITGESAWLDAATGLLDTALEH
                     FADPERPGRWFDTADDAERLLVRPADPLDGATPSGASSIAEALLTAAHLVPAPRADRY
                     ATAAAESLATATLVLAKLPRSGGHWLAVAEAAVRGPIQIAVATHDSDSALLRAARELA
                     PGGSVVVGGAADSSELLIDRDRIDGADAAYVCRGRVCDLPVTTAGDLAAALGAAV"
BASE COUNT          549 a         1238 c         1245 g          602 t
ORIGIN      
        1 acggcggcca gcacgagcac cggtttgcgc ggatcacggg ccagcagata caggcccaac
       61 caccagctga gcgagaagac caacgccgac agcgccgcca tggctcaagt gtggcatgcc
      121 gctggggaac cttcagcggc cgtaccgacg gtgccgtgcg gtgtaatcgc gaagcgcacg
      181 caggaaatcc acccgccgga attccggcca gtaggcctcc gtgaaccaca tctccgaata
      241 cgcgctctgc cacagcagga atccggacag ccgctgctcc cccgacgtgc ggatcaccag
      301 atccggatcg ggctgaccgg aggtgtacag gttctccgag atcgcgtcgg cggtgaccgc
      361 ctcgatcaac tgctcgccgt tcgcgccgtt ggcgatctcc ttgccgagca gcgcccgcac
      421 cgcgccgacg atctcttggc gcccgccgta gccgacggcg acgttcacat gaaagccgcc
      481 gtggccgttg gtcgattcca cggcgtcgcg cagccgccgc gccggctcct cgccgagcag
      541 ttcgaggtcg ccgacggttc gcacactcca cctgttggcg ggcgcacaga tctcctccac
      601 cacgtcggtg atgatgtcga tcagcgacga cagctcctcg ggttcgcgct gcaggttctc
      661 cgtcgaaagc agatagacgg tggccatctc gatgccggcc tcctggcacc agcgcatcat
      721 ttctgcgatc ttcgtcgcac ccgcccgata gccgtaactc acgtcatcgt gaccagcatc
      781 acgggcccat cgccggttgc cgtcgcacag caccgcgata tggcggggca gttcggattt
      841 cgacgccgtg agaccctgcc gcagccgcat ctcgtagagc cggtacatcg gctccttgag
      901 acgagcggga atgatctcca cgaaaatcca gactactgtg gcggtcggaa atcaccgaga
      961 ggcaatgggg gtgacatgac cgcctcgatc gataccgaag aacctgatcg cacacagcgg
     1021 gccggcgacg gcgccgagga cttccccgag gccgtcgtcg accgggttgc ccacgccatc
     1081 ggcaagccga gggcgcgggg ctggatccat gtctacgcgg ccgtcgtcgc actcatcgcg
     1141 ggcgcggcgc tggtgtcggt gtcctgggcg atggccggaa cgcgagccgg gctcgcgacg
     1201 ctcatctaca cgctgacgat cgtggccatg ttcgccgtca gcggcgccta tcaccggatc
     1261 aactggaagt cggagacgct gcgcaagtgg atgaagcgcg ccgaccactc gatgatcttc
     1321 gtgttcatcg cgggcagcta cacgccgttc gccctgctgg cattgccgtc cgagagcggc
     1381 atggtgctgc tctggatcgt ctggagcggg gcgctcgccg gcgttctgct caagatgttc
     1441 tggccgtcgg cgcctcgctg ggtgggcgtg ccgctctacc tgctgctggg ctgggtggcg
     1501 gcgtggttcg tcggcccgat cctcgatggc gccggcgtgg cggcgcttgt cctgctgatc
     1561 gtcggcggcg cgctctacag cgtcggcggc gtcctgtacg gcctcaagtg gcccgacccg
     1621 tggccggcga cgttcggcca tcacgagttc ttccacgcct gcacggcggt ggcggcgatc
     1681 tgccactacg tcgcgatgtg gttcgccgtt ttcaggggct gacttcgccg gcagccatcg
     1741 gcttgatgtt ctccgggctc cagtaggcct tcatcgaggc gatcttgccg tcctcgttga
     1801 aggtcatggt gctgatgatc tcgatgcgca ctccgccttc gccgagatcg atgatgagcg
     1861 cccatgagaa cgccgcctcg ttgcccaggg tgcgcagcga gtcgagttcg gtcgacttgt
     1921 ccgtgtcggg gatctgcgag tagaagccgt ggatcgcctg gcgcccgatg tgcacctcgc
     1981 tcccgacagg atcctcgacg gttccgtcgt cggcgtagag ctcgacgatg gcctcgacat
     2041 tgccctgcgc ggcgagctcg aggtagcgct tgacggtctt ggcgttgtct tcgggcgtcg
     2101 acatgcggcg aacgctacac ggccgcaccc aaagcagctg cgagatcccc ggcggtggtg
     2161 accggcaggt cgcacacccg tccgcggcac acgtaggcgg catcggcgcc gtcgatgcgg
     2221 tcgcgatcga tcagcagctc cgaggagtcg gcggcgccgc cgacgaccac cgagccgccg
     2281 ggcgccagct cacgggcggc gcgcagcagt gccgagtcgg aatcgtgtgt cgcgacggcg
     2341 atttggatgg gaccgcgtac cgcggcctcg gcgaccgcca gccagtggcc gcccgatcgc
     2401 ggcaacttcg ccagcacgag tgtcgccgtc gccaacgact ccgccgcggc cgtcgcgtac
     2461 cggtctgctc gcggcgcggg caccagatgg gcggccgtga gcaacgcctc ggcgatcgac
     2521 gaggcacccg acggtgtcgc tccgtccagc gggtcggcag gccggaccaa gagccgctcc
     2581 gcgtcgtctg cggtgtcaaa ccaccggccg gggcgctcgg ggtcggcgaa gtgctcaagc
     2641 gcggtgtcga gcagtccggt ggcggcgtcc agccacgctg attcgccggt gatctggtgc
     2701 aatgtgagaa gtccggtggc gagcgtcgcg tgatcctcca ggatcgcggc gctgtcgccg
     2761 acgtgggcgg cgaggctggc gcggcgcagt cgaccgtctg cgatgtgcag gccgagcagc
     2821 acccgcgcgc atcgcgttgc ggcgtcgagg aattcgggct cgtcgagtgc gacgctcgcc
     2881 tcggccagcg cggtgatggc ccaaccgttc catgccgtga tgaccttgtc gtcgcggcct
     2941 ggctgcgggc ggatcgaccg ggcggccagc agggcggtcc tcacccgatc gaatcgcccc
     3001 gggtcgtcgg ggtcggcggg cagctgcagc acggagctgc cgtgctcgaa ggtgcccgct
     3061 tctgtcacgc cgaaaagcga cgcggcccaa gccccgtcgt ctgcaccgag cacctcggtg
     3121 agctggccgg gcgtccatgc gtaggtcagg ccttccacac ctgccgcgtc cgcgtccagc
     3181 gacgagacga acattccgtc gacgccaagt tcgtcgatca tgaagcgagc ggtctcgcgg
     3241 actactctgc gcgccaattg gtttccggtg cgccgcgcca agtgggcgta ggcccgcagc
     3301 agcagcgcat tgtcgtagag catcttctcg aagtgcggca ccacccagga gtcgtcgacg
     3361 ctgtaccggg cgaagccgcc tgcgagctgg tcgtagatgc caccgcgggc catcgcggcg
     3421 gcggtgcgtt cgacggccgc caacggcgcg ggctcgccgg tgcgttcgtg gctacgcagc
     3481 agcgcctcca gcaacgccga gggtggaaac ttgggcgcac cttgggaacc ggttgagaat
     3541 ccgcctcgtt caacatcctc gtcgtcaagc accgcggcga cggcctggtc gcacaaggcc
     3601 ggctgcacct cgggtccgcc gccgggtagc ccgg
//
LOCUS       DASYLX010000948         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2581956,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000948 DASYLX010000000
VERSION     DASYLX010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581956"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VGP27_22155"
     CDS             complement(<1..617)
                     /locus_tag="VGP27_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7854033.1"
                     /translation="MRAPLEPRIKPAQDPASVRTAGGHRLSVLLVEDDPGDALLVEEL
                     IADAGADFTCAWAQSMSAAEHELALTHPDCVLLDLNLPDANGIVAVERIKQMDPNIPI
                     VVLTGLSDEHFGTSAVASGAQDYLIKGRVEPEMLRRAVLYAIERKRAEITAVQLHASQ
                     LRAMENARLERGLSPSPLLLENPGVEIIARYVPSRENTLLGGDFY"
     gene            832..>1849
                     /locus_tag="VGP27_22160"
     CDS             832..>1849
                     /locus_tag="VGP27_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854034.1"
                     /translation="MSTVADPDRVADLARKVVADHDPKSVPVPEFLGACYDAGLSWVH
                     FPEGLGGLGVSRGLQAVADGILQGAGGPVPLGLNPMGYGMAAPTVREHAQTDDVKREL
                     LRPLATTEHIWCQLFSEPGAGSDLAGLATSAVPDGDEWVVNGQKVWTSLAHRARWGLL
                     LARTNPDLPKHKGLTYFVIDMHGPGVETRPLRQLTGHAEFNEVYMTDARIPDTHRLGG
                     VGDGWRVAMTTLMNERSALGASGSRRGAGTIADAVSLWASRPDLQTPVLRDRLTQLWL
                     RSEAQRLTSERSHAAATVGGPGPEGSIGKLVGAELNQHIYQWCMDLLGPEGILYHGYG
                     MSDGA"
BASE COUNT          298 a          609 c          637 g          305 t
ORIGIN      
        1 tcgtagaagt cgccgccgag cagcgtgttc tcccggctgg gcacgtacct cgcgatgatc
       61 tcgacaccgg ggttctcgag cagtagaggc gacggcgaaa ggccgcgctc gagccgggcg
      121 ttctccatgg cccgcagttg actggcgtgc agctggaccg cggtgatctc ggcgcgcttg
      181 cgttcgatcg catacagcac cgcccggcgc aacatctcgg gttcgacccg gcccttgatc
      241 aggtagtcct gggcgcccga ggccaccgcc gacgtaccga agtgctcgtc ggacaacccg
      301 gtgaggacga cgatcgggat gttggggtcc atctgcttga tgcgctcaac ggcgacgatc
      361 ccgttggcgt cgggcagatt gaggtcaagc agcacgcagt cggggtgggt gagcgcaagc
      421 tcatgctccg cggcggacat cgattgagcc catgcacagg tgaagtccgc gcccgcgtcg
      481 gcgatcagtt cctctaccag aagtgcgtcg cccgggtcgt cctcgacgag cagtaccgac
      541 agacgatgtc cgcctgccgt acggaccgat gcagggtcct gtgcaggctt gattcgcggc
      601 tcaagcggcg cgcgcatggg tcatcacgtc ctttacgtaa cgccacccgg gtcgcgctga
      661 ccttctgctt cagtgacaac gactacaaag gataccgagc cgccctgcaa gccgctgcgg
      721 tgtcccccga tacggttcga tgatcggcgt ttcctatcgt ggctgcatga gcgaagcttg
      781 cgagcgaata tcgcgcacgg aatgcggcgg ccgagcctgc gaggtcggcg catgagcacc
      841 gttgccgacc cggatcgcgt cgccgatctc gccaggaagg tcgtggccga ccacgacccg
      901 aagtccgttc ccgtcccgga gtttctcggc gcctgttatg acgccgggtt gtcctgggtg
      961 catttccccg aaggccttgg cggtctcggg gtttcgcgcg gtctgcaggc ggttgccgac
     1021 ggcatcttgc agggtgcggg cggtccggta ccgctcggcc tcaacccgat gggctacggg
     1081 atggcggcgc cgaccgtgcg tgaacacgcc cagaccgacg acgtgaagcg cgagctgctg
     1141 cgtccgctgg cgaccaccga gcacatatgg tgtcagctgt tctctgagcc cggcgcgggt
     1201 tcggacctcg ccgggttggc gacctcggcg gtgcccgacg gtgacgaatg ggtcgtcaac
     1261 ggccagaagg tgtggaccag cctggcacat cgcgcgcgat ggggtctgct gctcgcacgc
     1321 accaaccccg acctgccgaa gcacaagggc ctcacctact tcgtgatcga catgcacggg
     1381 ccgggcgtgg agaccaggcc gctgcgccag ctgaccgggc acgcggagtt caacgaggtc
     1441 tacatgaccg acgcccgcat ccccgacacg caccggctcg gaggcgtcgg cgacgggtgg
     1501 cgggtcgcca tgaccacgct gatgaacgag cgcagtgccc tcggcgccag cggaagcagg
     1561 cgcggtgcgg gcacgatcgc cgacgccgtc tcactgtggg cgtcgcgacc ggatctgcag
     1621 acacccgtac tgcgggaccg gctcacgcaa ctgtggctgc gctcggaggc gcagcgcctc
     1681 acgtcggagc gctcgcatgc cgccgccacg gtcggcggcc ccggccctga aggctccatc
     1741 ggcaagctgg tcggcgccga gctgaaccag cacatctacc agtggtgcat ggatttgctt
     1801 gggccggaag ggatcctgta ccacggctat ggcatgagtg acggcgcgg
//
LOCUS       DASYLX010000949         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1704748,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000949 DASYLX010000000
VERSION     DASYLX010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704748"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VGP27_22165"
     CDS             complement(<1..150)
                     /locus_tag="VGP27_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate synthase"
                     /protein_id="HEV7854035.1"
                     /translation="MTSPVPTPDEIASLLQVEHLLDQRWPETKLEPSTARISALLELL
                     GSPQRS"
     gene            complement(147..>1893)
                     /locus_tag="VGP27_22170"
     CDS             complement(147..>1893)
                     /locus_tag="VGP27_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7854036.1"
                     /translation="PTIMDAKGRIADTGTEFDGMDRFDARVAVREALAAQGRVVEEKR
                     PYLHSVGHSERSGEPIEPRLSLQWWVKVESLASAAGDAVRNGHTVIHPASLEPRWFAW
                     VDNMHDWCISRQLWWGHRIPIWHGPNGETVCVGPDETPPEGWEQDPDVLDTWFSSALW
                     PFSTMGWPAHTPELAKFYPTSVLVTGYDILFFWVARMMMFGQFVADDPAITLDGKRGP
                     QVPFENVFLHGLIRDEFGRKMSKSRGNGIDPLDWVEMFGADALRFTLARGASPGGDLS
                     IGEDHARASRNFATKLFNATRFALMNGAAPAPLPDAAELTDADRWILGRAEGVRAEVD
                     SAFDSYEFSRACEALYHFAWDEFCDWYVELAKVQLADGVTHTTAVLAEVLDTLLKLLH
                     PVMPFVTETLWKALTGGESLVIAQWPSPSGFAVDQVAAQRITDMQKLVTEVRRFRSDQ
                     GLADRQKVPARLTDVTAADLDSQVPAVTSLAWLTPASDGFSPTASVEVRLTHGTVVVE
                     LDTSGTVDVAAERRRLEKDLAAAEKELKGTTAKLDNEAFLAKAPADVVDKIRGRRSLA
                     QEEVERINSRLASLR"
BASE COUNT          312 a          634 c          631 g          316 t
ORIGIN      
        1 gctgcgctgc ggcgagccga gcagttccag cagcgccgaa atgcgggccg tgctcggttc
       61 gagcttggtt tccggccagc gctggtcgag aaggtgctcg acttggagca gcgaggcgat
      121 ttcgtccggc gtcgggacgg ggctggtcat cgcagagacg ccagcctcga gttgatccgc
      181 tcgacttcct cctgagccag cgatcggcgg ccgcgaatct tgtcgacgac gtcggcgggc
      241 gccttggcca gaaaggcttc gttgtccagc ttggcggtgg tgcccttgag ttccttctcc
      301 gcggcggcca gatccttctc cagccggcgg cgctctgccg cgacgtcgac agtccccgac
      361 gtatccaact cgaccacgac ggtgccgtgc gtcagccgca cctccaccga ggcggtcgga
      421 gagaaaccgt cggaggccgg cgtcagccac gccagcgatg tcaccgcagg cacctgcgag
      481 tcgaggtcgg ccgcagtgac gtccgtcagc cgtgccggca ccttctgccg gtcggcgagc
      541 ccttggtcgc tgcggaaccg ccgcacctcg gtgaccagct tttgcatatc ggtgatgcgc
      601 tgggccgcaa cctggtccac cgcgaaaccg gacgggctcg gccactgtgc gatgaccagg
      661 gactcgccgc cggtcagtgc cttccacaac gtctcggtga cgaacggcat cactggatgc
      721 aacagcttga gcagcgtatc gagcacctcg gcgagcaccg ccgttgtgtg ggtgactccg
      781 tcggcgagct gaaccttcgc aagctcgacg taccagtcgc agaactcgtc ccaggcgaag
      841 tgatagagcg cctcgcaggc gcggctgaac tcgtagctgt cgaacgccga atcgacctcg
      901 gcgcgaaccc cttcggctcg tccgaggatc caacggtcgg cgtcggtgag ttcggccgca
      961 tcgggcagcg gggctggggc ggccccgttc atcagcgcga aacgggtggc gttgaacagc
     1021 ttggttgcga agttacggga cgcgcgggcg tggtcctccc cgatggacag gtcaccgccg
     1081 gggctggcgc cgcgggcgag ggtgaatcgc aacgcgtcgg cgccgaacat ctccacccaa
     1141 tccagaggat cgatgccgtt gccgcgcgac ttactcatct tgcggccgaa ctcgtctcga
     1201 atcaggccgt gcagaaagac gttctcgaac ggcacctggg gaccgcgctt gccatccaga
     1261 gtgattgcgg ggtcgtcggc gacgaactgg ccgaacatca tcatcctggc cacccagaag
     1321 aacaggatgt cgtagccggt gacgagcacg ctggtcggat agaacttcgc cagttccgga
     1381 gtgtgggcgg gccagcccat cgtggagaac ggccacagcg ccgaggagaa ccacgtgtcc
     1441 agcacatcag ggtcctgttc ccagccctcg ggcggtgtct cgtccgggcc gacgcagacg
     1501 gtctcgccgt tggggccgtg ccagatcggg atgcggtgtc cccaccacag ctgccgggat
     1561 atgcaccagt cgtgcatatt gtcgacccag gcgaaccacc gcggctccag gctcgccgga
     1621 tgaatcacgg tgtgcccgtt gcgcaccgcg tcgcctgcgg ccgaggccaa cgactcgacc
     1681 ttgacccacc attgaagcga cagccgaggt tcgatgggct ccccgctgcg ctcggaatgc
     1741 ccgacgctgt gaagatacgg tcgcttctcc tcgacgaccc ggccctgtgc ggccagcgcc
     1801 tcgcgaaccg ccacgcgggc gtcgaagcga tccatgccgt cgaattcggt accggtgtcg
     1861 gcgatccgac ccttggcgtc catgatcgtc ggc
//
LOCUS       DASYLX010000950         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1155921,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000950 DASYLX010000000
VERSION     DASYLX010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155921"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VGP27_22175"
     CDS             complement(<1..539)
                     /locus_tag="VGP27_22175"
                     /EC_number="1.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family mycofactocin-dependent
                     oxidoreductase"
                     /protein_id="HEV7854037.1"
                     /translation="MAASDGPLEGRVAFITGAARGQGRAHAIRLANDGADIIAIDVCR
                     PVSDTVTYPLPTSEDLAETVRAVEATGRKVLAREVDIRDLAALQKVVADGVEQFGRLD
                     IVVANAGVLSWGRLFEMSEEQWDTVIDINLNGTWKTIRAAVPAMIEAGNGGSIVIVSS
                     SAGLKATPGNGHYSASKHGL"
     gene            complement(695..1939)
                     /gene="arcA"
                     /locus_tag="VGP27_22180"
     CDS             complement(695..1939)
                     /gene="arcA"
                     /locus_tag="VGP27_22180"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV7854038.1"
                     /translation="MTNPSASYGVHSEVGKLRKVLVCSPGLAHERLTPTNCDDLLFDD
                     VLWVQNARRDHFDFMDKMRDRDVEVVELHDLLTETMAIPEAKSWLLDRKIVANEVGLG
                     LVDDTRGFLDSLQPRRLSELLIGGMSTRDLPAEIQSGYRALAREDTGVTEYLMPPLPN
                     TLYTRDTTCWIYGGLTLNPLFWPARHDETLLMKAIYEFHPDYIGSAVWWGDPEKVWGL
                     ATLEGGDVLIPGNGVVLIGMSERSSRQAITQVAAELFKRGAAENVIVAGMPKLRAAMH
                     LDTVFTFADRDVVTIYPEIVDSIASFTLMPSDSGVGVDVIEETKPFVEVVAAALELSH
                     LRVVETGGSKYDYERQQWDSGNNLVAVEPGVVFAYDRNTHTNSLLRKAGIEVITIVGA
                     ELGRGRGGGHCMTCPIIRDPVD"
     gene            complement(1939..>2548)
                     /locus_tag="VGP27_22185"
     CDS             complement(1939..>2548)
                     /locus_tag="VGP27_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV7854039.1"
                     /translation="AQITGMGETGTRINDQPVVKLQLHIEGPGIAPFDSQDRVIASVT
                     RLGNLTNRKLVVLVDPTTQQYQIDWERSALINGLVPATFTVAEDNRTYDLSGQTGALM
                     EILQILKANGIGMNNMIDLRSNPAARQQVQAIVQRAAAQQAPVRPGPPPTSGAPPPPQ
                     YPAMAPEPSTSQRLQELETLRATGAVSEEEYNAKRRQILSDL"
BASE COUNT          448 a          806 c          793 g          501 t
ORIGIN      
        1 aggccgtgct tcgacgccga atagtggccg ttaccgggag tggccttcag acccgccgat
       61 gagctgacga tgacgatcga gccgccgttg ccggcctcga tcatcgcggg caccgccgcg
      121 cggatcgtct tccaggtgcc gttcagattg atgtcgatga cggtgtccca ttgctcctct
      181 gacatctcga acaaccgtcc ccagctcaac acaccggcgt tggcgaccac gatgtccagc
      241 cggccgaact gctcgacacc gtcggcgaca accttctgca acgcagccag gtcgcgaatg
      301 tccacttccc gggccagcac cttgcgcccg gtggcttcca ccgcccggac cgtttcggcg
      361 aggtcctcgg aagtgggtag cggataggtg acggtgtcgg aaactgggcg gcagacatcg
      421 atcgcgatga tgtcggcgcc atcgttggcc agccgaatcg cgtgagcccg gccctggccg
      481 cgcgcggccc cggtgatgaa tgccactcgc ccttccagag ggccgtccga cgccgccatc
      541 actgctcctt tcgtgcactg cggaccaggc taacagccga actggaacgt gttctaggaa
      601 ccgcggcgca tccgcttcac ttcggggcag ggcgccggag tgcggtcctt cgaagtgcca
      661 gcctcacagc gactaattcg ctcgtacaga cgcgctaatc gacggggtcg cggatgatcg
      721 ggcaggtcat gcagtgccct ccgccgcggc cgcggcccag ttccgcccca acgatggtga
      781 tgacctctat tcctgccttg cgcagcaggg aattggtgtg agtgttgcgg tcataggcga
      841 acacaactcc gggttcgacg gcgacgaggt tgttgccgct gtcccattgc tgacgctcgt
      901 agtcgtactt gctcccaccc gtctcaacga ccctcagatg cgacaactcc aatgcagccg
      961 caaccacttc gacgaacggc ttggtctcct cgatcacgtc aacaccgacg ccggagtcgc
     1021 tgggcatcaa cgtgaacgat gcgatgctgt cgacgatctc ggggtagatc gtgactacgt
     1081 cgcggtcggc gaaggtgaac acagtgtcca agtgcatggc cgcgcgaagc ttgggcatcc
     1141 cggcgacaat cacgttctcg gcggcacccc gtttgaacag ctcggctgcg acctgggtga
     1201 tggcctgccg tgaggatcgc tcgctcatgc cgatgagcac caccccgtta ccgggtatca
     1261 gtacgtcgcc accctccaaa gtagctaacc cccagacctt ttcgggatct ccccaccaca
     1321 ccgcggagcc gatgtagtca ggatggaact cgtagatcgc cttcatgagc agtgtttcgt
     1381 cgtgtcgggc gggccagaac aacggattga gggtgaggcc gccgtaaatc cagcacgtgg
     1441 tgtcacgcgt atagagcgtg ttcggcagcg gtggcatcag gtattcggtc actccggtgt
     1501 cctcgcgcgc cagcgcgcgg tagccggact ggatctcggc aggcaggtcg cgcgtcgaca
     1561 ttccgccgat cagcagctcg gagagcctgc gcggttgcag cgaatcaagg aatccgcggg
     1621 tgtcgtcgac gagtcccagg ccgacttcat tcgcaacgat cttgcggtcc agtaaccaac
     1681 tcttggcttc ggggatcgcc atggtctcgg tcaacaggtc atgtagctct acgacttcca
     1741 cgtcgcggtc acgcatcttg tccatgaagt cgaagtggtc gcggcgggcg ttctgcaccc
     1801 acagcacatc gtcgaacagt aggtcgtcgc agttcgtcgg ggtgagtcgt tcatgcgcaa
     1861 gccccggaga gcacaccagc accttgcgca gcttgccgac ctcggagtga acgccatagc
     1921 tggcggacgg attcgtcatc acagatcgga gagaatctgc ctgcgtttcg cgttgtactc
     1981 ctcttcggag acggcaccgg tcgcgcgcag ggtctccagt tcctgcaacc gctgtgacgt
     2041 ggacggctcc ggcgccatgg ccgggtactg aggtggaggt ggtgcgcccg aagtaggtgg
     2101 cggcccgggc ctaacaggtg cctgttgcgc cgccgctcgc tggacaatcg cctgaacctg
     2161 ttgacgcgca gcaggattgg accgcaaatc gatcatattg ttcatgccga tgccattcgc
     2221 cttgaggatc tgcaagatct ccatcaaggc gccggtctga ccgctgaggt cgtaggtcct
     2281 gttgtcctcg gcgactgtga acgtcgcagg caccagcccg ttgatcagtg cgctgcgttc
     2341 ccaatcgatt tggtattgct gggtggtcgg gtcgacgagc acgacgagct tgcgattggt
     2401 gaggttaccc agccgcgtca cactggcgat gacccggtct tgtgaatcga acggtgcgat
     2461 tccgggtccc tcgatgtgca gttgcagctt caccaccggc tgatcgttga tacgggtgcc
     2521 cgtctcaccc atcccggtga tctgggcg
//
LOCUS       DASYLX010000951         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1704847,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000951 DASYLX010000000
VERSION     DASYLX010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704847"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VGP27_22190"
     CDS             complement(<1..1395)
                     /locus_tag="VGP27_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854040.1"
                     /translation="MHRRRLVRALCTSTVVVSAFLTGCAPVLAADPRYATDSGARPQG
                     QPTTNNKAGGPPAIEAPKNDLSWRNCTSQVFSGSAVDPLPGVTLDCATYDADLDPING
                     ATGSVSIGVVRARSVQTPEDAGPLVMTTGSDLPTSVQLPIWLSRAGADVLKSHPIVAV
                     DRRGMGMSSPIECRDLFDRQEMLDQAQFQSGDDPVANLAAITMTATTSCTDTIAPGDS
                     AYDNSHAAEDIERLRSTWDVPSIALLGIGNGAQVALAYAGSHPGKVARLVLDSPLPLG
                     IAAEAATEQRVKGEQTALDAFTSQCAATNCPLGSDPKAAVDALLADARAGNGPGGASV
                     STVADAIATALAYPRGDRAAATNSLASAVATARSGDANQMNNLINQADSLRQTDGQFV
                     NSCSDALNRPTPDRVRELVVAWPKLYPQFGAVGALSMVKCLNWPSGSAPQDPKNLKIP
                     VLLMGVQNDPIVGNE"
     gene            complement(1421..2194)
                     /locus_tag="VGP27_22195"
     CDS             complement(1421..2194)
                     /locus_tag="VGP27_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HEV7854041.1"
                     /translation="MPDDAAGVRYTVLDSTDSVDENRLPDFYAYPDNLQSCWLRGNMI
                     ASLDGGATADGKSGSLAGPGDRAVFAEMRQLADVILVGAGTVRSENYSGAQLSPAQRQ
                     ARQRRGQDEVPPIAVVTNRAALHHDARIFTRTEVAPLILTSAEAVPDAQSRLGSVAEI
                     IDASGPHPDGVDAATVLKILAERRLLRVLAEGGPRFLGSLIEQDLLDELCLTIAPLLV
                     GGGARRIATGPGQVHTRMRRSHLLTDEAGYLYTRYVRGG"
     gene            2229..3302
                     /gene="zapE"
                     /locus_tag="VGP27_22200"
     CDS             2229..3302
                     /gene="zapE"
                     /locus_tag="VGP27_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEV7854042.1"
                     /translation="MQSAAHGRSSSAGGVGRLVDRHPRVTPERLIAQLHPPPTFADVS
                     FESYQPDPAEPSQSAAVQSCMQFCDDAVARRAGRKKLFGKREVLPGVGLYLDGGFGVG
                     KTHLLASSYYRISAASSAPTAFATFGELTQLASVFGFVECIDLLADYAVVCIDEFELD
                     DPGNTTLISRLLSQLVERGVSIAATSNTLPEQLGEGRFAAQDFLREINTLAAIFKAVR
                     IEGPDYRHRDLPPAPEPLSDKEVTARAAEVPGATLDDFDDLCAHLATMHPSRYLTLIE
                     GVSAVFLTGVHQIDDQNVALRLVSLTDRLYDAGVPVVASGSKLDTVFGEEMLAGGFRK
                     KYLRATSRLLALTTAGQTGRTIT"
     gene            complement(3278..3808)
                     /locus_tag="VGP27_22205"
     CDS             complement(3278..3808)
                     /locus_tag="VGP27_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7854043.1"
                     /translation="MTLEVTAAAAADLPELADVAAETFPLACPPSVTHQNIAAFIAEN
                     LTQEHFAGYLTDPDRLVLVARRDGRMIGYAVLIRGVPDDDDVNRAVTARPAVEISKMY
                     VLPDDHGAGASAALMIEAIRLAQELDAKSVWLGVNQRNQRAQRFYAKHGFTINGTKTF
                     RLGTRTENDYVMVRPV"
     gene            complement(3816..4307)
                     /locus_tag="VGP27_22210"
     CDS             complement(3816..4307)
                     /locus_tag="VGP27_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854044.1"
                     /translation="MRRWLVLLFSALTISGAVLAGPASAVDAPIGRLGETLRVELMGV
                     VADVTVHDILPSDVPPGFGYPPRAPRYQVYRANVTIHVVETPAPYAVGIAFTFKGVTP
                     TGDAYEPRSSDAPDALQSTMLNAPAGATVNGGVWWDCYRDLVSNVVLVDRKTGYHLAQ
                     WNV"
     gene            complement(4375..4794)
                     /locus_tag="VGP27_22215"
     CDS             complement(4375..4794)
                     /locus_tag="VGP27_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7854045.1"
                     /translation="MKLNDAAREFIGEGVDATLVTINPDGSPQATVVWVALESTPDGD
                     ELVSAHLAEYRKTRNVRRDPRVAVTIVRPTRGEQAPYLAVSGTARITEGGAPELLKRL
                     AKVMLGSDEHFPPPNSPPGLLTRIRIDKVGGNGSWTA"
     gene            complement(4823..>5228)
                     /locus_tag="VGP27_22220"
     CDS             complement(4823..>5228)
                     /locus_tag="VGP27_22220"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV7854046.1"
                     /translation="HDAVLTKLAAAVSPRVLRAPARLDATARQLDEYFAKRRTAFDVP
                     VDLRLAEGFRRSVIEHLRDIDYGRRQSYAEVAAAVGSPRAVRAVGTACAHNPLPVVIP
                     CHRVVRSDGSTGQYIGGAAAKSALLGLETTGE"
BASE COUNT          790 a         1694 c         1866 g          878 t
ORIGIN      
        1 ttcgttgccg acgatcgggt cgttctgcac gcccatcagc agcacgggga tcttgaggtt
       61 cttcgggtcc tgcggcgccg atccgctcgg ccagttcagg cacttgacca tgctgagcgc
      121 gccgacggcg ccgaattgcg gatagagctt gggccatgcg acgacgagtt cgcggacgcg
      181 gtcgggggta gggcggttca gcgcgtcgct gcacgagttg acgaattggc cgtcggtctg
      241 gcgcaggctg tcggcctggt tgatcaggtt gttcatctgg ttggcatcgc cgctgcgcgc
      301 cgtcgccacc gcggaggcca agctgttggt tgcggccgcg cggtcgccgc gcgggtaggc
      361 cagtgcggtg gcgatcgcgt cggcgacggt ggaaaccgac gctccgccgg gcccgttgcc
      421 tgcccgcgcg tcggcgagca gggcgtccac ggcggccttc ggatccgacc cgagagggca
      481 attggtcgcc gcgcattgcg acgtgaacgc gtcgagcgcg gtctgctcac ccttgacgcg
      541 ctgttcggtc gcggcctcgg cggcgatacc gagcggcagc ggcgagtcga gcaccagccg
      601 cgccaccttg ccgggatgcg agcccgcata ggccagcgcc acctgcgcgc cgttgccgat
      661 gccgagcagc gcgatcgacg gcacgtccca ggtgctgcgc agccgttcga tgtcctcggc
      721 ggcgtgcgag ttgtcatagg ccgagtcgcc gggggcgatg gtgtcggtgc agctggtggt
      781 ggccgtcatg gtgatcgccg cgaggttggc gacgggatcg tcgccggact ggaactgcgc
      841 ctggtcgagc atttcctgac ggtcgaacag gtcgcggcac tcgatcgggc tggacatgcc
      901 cataccgcgc cggtcgaccg cgacgatcgg atggctcttg agcacgtcgg cgcccgcccg
      961 cgacagccaa atcggcagct gcaccgaggt gggcaggtcc gaacccgttg tcatcaccag
     1021 cggtccggcg tcttcgggcg tctgcaccga ccgcgcacgc accacgccga tgctcaccga
     1081 tccggtggcg ccgttgatcg ggtcgaggtc ggcgtcgtag gtggcgcaat ccagcgtgac
     1141 gccgggaaga gggtcgacgg ccgagccgct gaacacctgt gaggtgcagt tgcgccagga
     1201 caggtcgttc ttgggggcct ctatcgcggg tggaccaccg gccttgttgt ttgtggtcgg
     1261 ctgcccctga ggtcgcgcgc ccgagtcggt ggcatagcgc ggatcggcgg ccagcacggg
     1321 cgcgcatccg gtcaggaagg ccgacacaac gacggtcgat gtgcacaggg cacggaccag
     1381 gcgacgccga tgcatggcca ccacagtact gatcaggctg tcagccccct cggacgtagc
     1441 gggtgtacag atagcccgcc tcgtcggtga gcaggtggct gcgtcgcatc cgggtgtgca
     1501 cctgaccggg gccggtggcg atgcgccggg cacccccgcc gaccagtagc ggcgcgatcg
     1561 tcaggcacaa ctcgtcgagc aggtcttgtt cgatcagcga gccgaggaac cgcgggccgc
     1621 cctcggcgag cacccgcaga agccggcgct cggcaaggat cttcagaacg gtcgcggcgt
     1681 cgaccccatc cggatgggga ccggatgcgt cgatgatctc ggcgaccgat cccagcctgc
     1741 tctgggcgtc tgggaccgcc tcggcggagg tgaggatcag cggggccacc tccgtgcggg
     1801 tgaagattct ggcgtcgtgg tggagcgcgg ctctgttcgt gacgacggcg atgggcggca
     1861 cctcgtcctg gccgcggcgc tgcctggcct ggcgctgcgc ggggctcaac tgtgcgcccg
     1921 aatagttctc gctacggacg gtgcccgcac cgaccaggat gacatcggcg agctggcgca
     1981 tctccgcgaa caccgcgcgg tcgccggggc cggccagact tcccgatttg ccgtcggcgg
     2041 tcgcaccgcc gtcgaggctg gcgatcatgt tgccgcgcag ccaacacgac tgcaggttgt
     2101 cggggtaggc atagaagtcc ggtaaccggt tctcgtcgac tgagtcggtg gaatcgagca
     2161 cggtgtatcg gaccccagca gcgtcgtcgg gcatgggcac gatctcagca cgtcgctaga
     2221 gttcgtgcat gcagtccgcc gcgcacggga ggagcagttc tgccggtggt gtcggccgtc
     2281 tcgtcgatcg gcatccacgc gtcaccccgg agcggttgat cgcccagctt cacccgccgc
     2341 cgacgttcgc cgacgtgagc ttcgagagct atcagccgga tccggccgag ccgtcgcagt
     2401 cggcggcagt gcagtcgtgc atgcagttct gcgacgacgc ggtggcgcgg cgggcaggca
     2461 ggaagaagtt gttcggcaag cgcgaggtgc tgccgggcgt cgggctctac cttgacggcg
     2521 gcttcggcgt cggcaagacc cacctgctgg cgtcgagcta ctaccggatc tcggccgcgt
     2581 cgtcggcgcc tacggcgttc gcgacgttcg gggaactcac gcagctggca agcgtcttcg
     2641 gtttcgtcga atgcatcgac ctgctggccg actacgcggt ggtgtgcatc gacgagttcg
     2701 agctggacga ccccggcaac accacgctga tctcacggct gctctcgcaa ctggtcgaac
     2761 gaggcgtttc gatcgccgcc acgtcgaaca cgctgcccga acagctgggg gagggccggt
     2821 tcgccgcgca ggatttcttg cgcgaaatca acacgctcgc agcgattttc aaggccgtgc
     2881 gcatcgaagg gccggactac cgtcaccgcg acctgccgcc cgcgccggag ccgctgtcgg
     2941 acaaggaggt caccgcccgc gccgcagagg tgcccggtgc caccctcgac gacttcgacg
     3001 atctgtgtgc gcacttggcg acgatgcatc cgtcgcgtta cctgacgttg atcgaaggcg
     3061 tttcggcggt gttcctcacc ggcgtgcatc agatcgacga ccagaacgtc gcgctgcggc
     3121 tggtatcgct caccgatcgc ctgtacgacg cgggtgtccc ggtggtggcg tcgggatcca
     3181 agctcgacac cgttttcggc gaggagatgt tggcgggcgg attccgcaag aagtacctgc
     3241 gggccacgtc gcggctgctg gccctgacga cggccggtca gaccgggcgc accatcacgt
     3301 agtcgttctc ggtgcgggtg ccgagccgga atgtcttggt gccgttgatc gtgaagccgt
     3361 gcttggcgta gaagcgctgt gcacgttggt ttcgttgatt gacgccaagc cacacactct
     3421 tggcgtcgag ttcctgcgcc agccgaatcg cctcgatcat cagtgcggcg gaggcgccgg
     3481 caccgtgatc gtcgggcagc acgtacatct tggagatctc cactgccggc cgggcggtga
     3541 ccgctcggtt gacatcgtcg tcgtcgggga caccgcgaat cagcacggcg tatccgatca
     3601 ttcggccgtc cctacgcgcg acgaggacca gtcgatcggg atcggtcagg tagccggcga
     3661 agtgttcctg tgtcaggttc tcggcgatga acgcagcaat gttctggtgt gtcaccgacg
     3721 gcgggcacgc caacggaaag gtttcggcgg cgacgtcggc cagttcgggt aggtccgcgg
     3781 cggcggcggc ggtgacttcg agcgtcaccg gccccctaca cgttccactg ggcgaggtga
     3841 tagccggtct tcctgtcgac gagcaccacg ttggacacca ggtcgcggta gcagtcccac
     3901 cacacgccgc cgttgaccgt cgctccggcg ggcgcgttca gcatggtgga ctgcagcgcg
     3961 tcgggagcgt cggagctccg cggctcgtat gcatcccctg tcggggtgac acccttgaag
     4021 gtgaaggcga tgccgaccgc gtacggggcg ggtgtctcga cgacgtggat cgtgacgttg
     4081 gcgcggtaca cctgatagcg cggtgcacgc ggcggatacc cgaatcccgg cgggacatcg
     4141 gagggcagga tgtcgtgcac ggtcacgtcg gcgacgacgc ccatgagttc gacgcgaagt
     4201 gtttcaccga ggcggccgat gggtgcatcg accgcagaag caggcccggc cagcacggca
     4261 ccggagatgg tcaaagcgga gaacagcagg accaaccagc gacgcatgtg aaatcccctc
     4321 cgtgtcgtct cgcatgatgg cacatgcccg ccacggcgga cagagggttg accgtcaggc
     4381 ggtccaggag ccgtttccgc ccaccttgtc gatccggata cgggtgagca gtcccggcgg
     4441 cgaattcggc ggcggaaagt gctcgtcgga gccgagcatc accttcgcca gcctcttgag
     4501 cagttcggga gcgccgccct cggtgatgcg tgccgtcccg ctgacggcga ggtagggcgc
     4561 ctgctcacca cgggtgggcc tgacgatggt taccgcaaca cgcggatcac ggcgcacgtt
     4621 gcgggtcttc cggtactcgg ccagatgtgc cgagacgagt tcgtcgccgt cgggcgtcga
     4681 ctccaacgcc acccatacga ccgtcgcctg cggactgccg tcgggattga tcgtcacaag
     4741 cgtggcgtca acgccctcgc cgatgaattc acgtgccgcg tcgttgagct tcatgcgttg
     4801 ttctaccgcc gcgcgggccg tttcattccc cggtggtctc caggccgagc agcgccgact
     4861 tggccgccgc ccccccgatg tactggcccg tcgacccgtc ggagcgaacc acgcggtgac
     4921 acgggatcac gacgggcaac gggttatggg cgcacgcggt gccgacggct cgcacggcgc
     4981 ggggactgcc gacggcggcg gccacctcgg cgtaactctg gcggcggccg tagtcgatgt
     5041 cgcgcaggtg ctcgatgaca ctgcggcgga agccctcggc gagtcgcaga tcgaccggca
     5101 catcgaaggc ggtgcgccgc ttggcgaagt actcgtcgag ttgacgggcg gtggcgtcca
     5161 accgggccgg cgcacgcagc acccgtgggc tcaccgcggc ggccagcttc gtcagcaccg
     5221 cgtcgtgg
//
LOCUS       DASYLX010000952         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1046437,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000952 DASYLX010000000
VERSION     DASYLX010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046437"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VGP27_22225"
     CDS             <1..1549
                     /locus_tag="VGP27_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854047.1"
                     /translation="ATSATFPDREALIECATGRRWTYSELHEATRRIATALIERGTKP
                     GDRIGIWSPNSAEWTMIQYATAQVGAILVNINPAYRTDELEYALNKASMCTVFAAPSF
                     KGSDYASMLDNVRARCPALSDVVVIGWEPWQEIAGTAPDADALARVQSGLTPSDPINI
                     QYTSGTTGFPKGATLTHTNILNNGYFVGELLGYTEQDRVCIPVPFYHCFGMVMGNLAC
                     TTHGAAMVIPAPGFDPEASLKAVEAQRCTSLYGVPTMFIAELGLPDFDGFDLSTLQTG
                     IMAGSTCPEQVMRNVIDRMHMTGVAICYGMTETSPVSTQTLPSDSLTRRVTTVGRVGP
                     HLEVQVVDDNGSQVQRGTPGELCTRGYSVMLGYWDDPEKTDEAIDADGWMHTGDIGVM
                     DDDGYLAITGRIKDMVIRGGENIYPREIEEFLYSHPDIVDAQVVGVADEKYGEELMAW
                     LRVKEGAEVPTPESLRAFCAGRIAHHKIPRYVQVIDEFPLTVTGKVRKVELRERGEAL
                     LATAPRV"
     gene            complement(1558..1770)
                     /locus_tag="VGP27_22230"
     CDS             complement(1558..1770)
                     /locus_tag="VGP27_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEV7854048.1"
                     /translation="MRLSTRNQLSGTITEVNLGAVMATVKVRLDGGDQVVTASITRDA
                     VEDLGLEVGKPATVLIKSTEVMIGVD"
     gene            1796..2368
                     /locus_tag="VGP27_22235"
     CDS             1796..2368
                     /locus_tag="VGP27_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854049.1"
                     /translation="MKIEGLRLRQRDSTTCGPSIAVVAGALVDPAYRKALTGSPHSPH
                     TRGAVKAWFDDEQLRIHTEVNRVWPRMLGTTPMGIARALTTRTQVRGVRYRWRLFRGR
                     RDRLTDVLHHVGTGWPVAMLVGRIIPRHWVLIVGVSGHTLSCYEPSSGELRPASLDAV
                     RRSRLTGVGYPRPFAFVLPSSKAATRSATS"
     gene            complement(2302..>2769)
                     /locus_tag="VGP27_22240"
     CDS             complement(2302..>2769)
                     /locus_tag="VGP27_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEV7854050.1"
                     /translation="HWHAEGAESVAIFAGPHAYISPSFYATKTDTGKVVPTWNYEILN
                     VYGSLVIHDDADWLLDLVTKLTNRHEAARPEPWQVTDAPESYTKSQLRGIVGIELAIA
                     KVEGKAKMSQNQPDSNRAGVIAGLRESDAPQDQLVADRVAAFEEGNTKANGRG"
BASE COUNT          486 a          878 c          933 g          472 t
ORIGIN      
        1 cgcaacgtcg gcgaccttcc ccgaccggga ggcgctcatc gagtgcgcga ccggccgccg
       61 ctggacctac agcgagcttc acgaagcgac cagaaggatc gccaccgcac tgatcgagcg
      121 cggcaccaag ccgggcgacc gaatcggcat ctggtcgccg aacagcgcgg agtggacgat
      181 gatccagtac gccaccgcgc aggtcggcgc gatcctggtc aacatcaacc ccgcgtaccg
      241 aaccgacgaa ctcgagtacg cactgaacaa agcctcgatg tgcacggtgt tcgcggcgcc
      301 gtccttcaag gggtccgact acgcgtcgat gctggacaac gtgcgagcac ggtgtcccgc
      361 gctcagcgac gtggtggtca tcgggtggga gccgtggcag gagatcgccg gcaccgcgcc
      421 cgacgccgat gcgctcgctc gggtgcagtc cgggttgaca ccgagcgatc cgatcaacat
      481 ccagtacacg tcgggcacca ccggatttcc gaagggcgcc acgctgactc acacgaacat
      541 cctcaacaac ggctacttcg tcggcgagct gctcgggtac accgagcagg acagggtctg
      601 catcccagtg cccttctacc actgcttcgg gatggtgatg gggaacctgg cctgcacgac
      661 gcacggtgcc gcgatggtga tccccgctcc cggattcgat cccgaagcaa gcctgaaagc
      721 ggttgaggcg cagcgctgta catcgttgta cggagttccg accatgttca tcgcggagct
      781 agggttgccc gacttcgacg gcttcgacct gagcacgttg cagaccggga tcatggccgg
      841 gtcgacatgc cccgaacagg tgatgcgcaa tgtgatcgac cggatgcaca tgacaggggt
      901 ggcgatctgt tacggcatga ccgagacgtc gccggtgtcg acccagacgc ttccgtcgga
      961 cagtttgacc cgaagggtga cgacggttgg acgggtcggg ccgcacctgg aggttcaggt
     1021 cgtcgacgat aacgggtcgc aggtgcagcg cggcaccccg ggcgaactgt gcacccgtgg
     1081 ctacagcgtg atgctcggct attgggacga cccggagaag acggacgagg cgatcgacgc
     1141 ggacggctgg atgcacaccg gcgacatcgg agtgatggac gacgacggtt acctcgcgat
     1201 caccggccgg atcaaggaca tggtgatccg cggcggcgag aacatctatc cccgcgagat
     1261 cgaggagttt ctgtacagcc atcccgacat cgtcgatgcg caggtggtcg gggtggccga
     1321 cgagaagtac ggcgaggagc tgatggcgtg gctgcgcgtc aaggaaggtg cagaagtccc
     1381 gacccccgaa agcctgcgcg cgttctgcgc cggccggatc gctcaccaca agattccgcg
     1441 gtacgtgcaa gtcatcgacg agttcccgct gaccgtgacg ggcaaggtca gaaaggtgga
     1501 gctacgcgag cgcggcgagg cgctgctcgc gactgctccg cgggtctgac cccggaacta
     1561 gtcgaccccg atcatcacct cggtcgactt gatcagcacc gtcgcgggct tgccgacctc
     1621 caagccgagg tcttcaacgg catccctggt gatcgatgcc gtgacgacct ggtcgccgcc
     1681 gtcgaggcgg accttgacgg tcgccatcac agcgccgagg ttgacctcgg tgatggtccc
     1741 gctcagctga tttcgagtgg atagccgcat atcgcaccct aacctttggg tggatatgaa
     1801 gatcgaaggt ctgcggttgc ggcagcgcga ctccaccacg tgcggtccca gcatcgcggt
     1861 ggtggccggc gcgctcgtcg acccggccta ccgcaaggcg ttgaccggct caccgcacag
     1921 cccgcacacg cgaggagcag tcaaagcctg gttcgacgat gagcagcttc ggatccacac
     1981 cgaggtgaac cgcgtctggc cgcgcatgct cgggaccacc ccgatgggga tcgcccgtgc
     2041 gctcaccacg cgcacgcagg tgcgaggcgt cagataccgg tggcggttgt tccgtggacg
     2101 gcgcgatcgg ctgaccgacg tgttgcatca cgtcgggacg ggctggccgg tcgcgatgct
     2161 cgtcggccga atcattccga ggcactgggt gttgatcgtc ggcgtcagcg ggcacacctt
     2221 gtcgtgttac gagccgtcgt cgggggagct acggcctgcg tccttggatg cagtgcggcg
     2281 ctcgcgcctg acgggagtgg gctacccgcg gccgttcgcc ttcgtgttgc cctcttcgaa
     2341 ggcggccacc cggtcggcga cgagctgatc ctgcggtgcg tcggactccc gcagaccggc
     2401 gatgacaccg gcgcggttcg agtccggctg attctgcgac atcttcgcct tgccttcgac
     2461 tttcgcgatg gcgagctcga ttccgacgat tccgcgcagt tgtgacttcg tatacgactc
     2521 gggcgcgtcg gtcacctgcc acggctcggg tcgggccgcc tcgtggcggt tggtcagctt
     2581 ggtgaccagg tcgagcaacc agtcggcatc gtcgtggatg accaggctcc cgtagacatt
     2641 gaggatctcg tagttccacg tgggaacgac cttgccggtg tccgtcttcg tcgcatagaa
     2701 actgggcgag atgtaggcgt gcgggcctgc gaagatcgca accgactcgg cgccctcggc
     2761 gtgccagtg
//
LOCUS       DASYLX010000953         6542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3679159,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000953 DASYLX010000000
VERSION     DASYLX010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679159"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VGP27_22245"
     CDS             complement(<1..212)
                     /locus_tag="VGP27_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HEV7854051.1"
                     /translation="MLFGLLRALARIRVTVVYAVILTCVTTALAVLGPQVQNRVITHA
                     STNLHNLSHGHLGTLFVSAFVIDAGP"
     gene            complement(224..1138)
                     /locus_tag="VGP27_22250"
     CDS             complement(224..1138)
                     /locus_tag="VGP27_22250"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7854052.1"
                     /translation="MDVESFDERSGAIGEIDQAPDLSASAQRGPGRWRSLRDSNHNPR
                     VVAFIRRARRALPGDPDFGDPLSAAGVGGPSAAARAADRLLERDAASREVSLAGLQVW
                     QALTERVSGKPANREVTLVFTDLVGFSSWALEAGDDATLKLLRRVAQVVEPPLLESGG
                     HIVKRMGDGTMAVFADPATAVRAVRTSLDAVKHVEVEGYTPRIRAGVHTGRPQRIGAD
                     WLGVDVNIAARMMERAARGGLVVSQATMDRIPEAEFEALGLTAKRIRKQVFSSKQDGV
                     PTDLAVYRVKTRRELPGGDEENGDIADS"
     gene            1291..2322
                     /locus_tag="VGP27_22255"
     CDS             1291..2322
                     /locus_tag="VGP27_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV7854053.1"
                     /translation="MTVSARTKRTTGADPLGPESLTWKYFGDLRTGMMGVWIGALQTM
                     YPELGAGVEEHSVLLREPLQRVARSVYPIMGVVYDGDRAGQTGRQIKGFHATIKGVDS
                     TGRRYHALNPETFYWAHATFFMLVIKVAEYFCGGLTDAEKRQLFDEHVQWYAQYGMSM
                     RPVPETWEEFCEYWDRVCSEDLEINRATLDILSIRIPKPWFVLMPTPIWDQLFKPMVA
                     GQRWIAAGLFDPVVREKAGMRWTAGDEVLLRLFGKLIEMAFVVVPDEIRLHPRALAAY
                     RRASGRIADDAPLVEAPAFMSPPRDRRDLPMHYVPRRKSLLDRAGSLVHTTFSLAGLR
                     PARGRSAAA"
     gene            2359..3612
                     /locus_tag="VGP27_22260"
     CDS             2359..3612
                     /locus_tag="VGP27_22260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854054.1"
                     /translation="MLEWSDVDIAVRDAVRDFVDKEVRPHVDALESGDMEPYPVIRKL
                     FTAFGIDALARESLEKRLARMREGADKSEGSGASGGMFGGDGSSAGMGFVVVSELCRV
                     CMGVVTGMGVSLGLTVPTIAGRGTLAQQERWLPGLVTYEKVGAWAITEPDSGSDAFGG
                     MKSYVVRDGDDYILNGQKTFITNGPDADVVVVYAKLDEGDGADRRDRKVLTFVLDRGM
                     EGFVQSKPLRKMGIHSSRTGELFFNNMRLGRDRLLGETEGDPSDSAGDGRASARSNFS
                     AERIGVAAMALGVIEECLKLCVDYAKTRKLWGQEIGQFQLIQLKLASMEVARINVRNM
                     LFRVIEAAQTGTQISLPEASAIKWYCSQAATDVAMDAVQLFGGNGYMTEYRVEQLARD
                     AKSLMIYAGSNEVQITHVARGLLSG"
     gene            complement(3691..4437)
                     /locus_tag="VGP27_22265"
     CDS             complement(3691..4437)
                     /locus_tag="VGP27_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV7854055.1"
                     /translation="MAAAIKLHRHVGRRRAARFLAEGPNLVEAALRRNLVIEVFVTEA
                     ASARFASLLTDVEVHIVSERAAKALSDTVTPVGLVAVCEVPSITLDDVLADEPRLVAV
                     AAEISEPGNAGTLIRIGDAMGADAVVMAGHSVDPYNGKCLRASAGSIFAVPVVSETDA
                     GAAVSALSEAGLRVLATTVDGSTSLDDTDLSGPTAWLFGPESHGLPPELAAMAAERIR
                     IPMPGAAESLNVASAAAICLYESAKAHRHH"
     gene            complement(4461..4880)
                     /gene="rplT"
                     /locus_tag="VGP27_22270"
     CDS             complement(4461..4880)
                     /gene="rplT"
                     /locus_tag="VGP27_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV7854056.1"
                     /translation="MARVKRAVNAQKKRRTVLEASKGYRGQRSRLYRKAKEQQLHSLN
                     YAYRDRRARKGEFRKLWISRINAAARANDITYNRLIQGLKAAGVEVDRKNLAEIAVSD
                     PAAFTALVDVARGALPEDVNAPGGAGRSATSDSGEAA"
     gene            complement(4930..5124)
                     /gene="rpmI"
                     /locus_tag="VGP27_22275"
     CDS             complement(4930..5124)
                     /gene="rpmI"
                     /locus_tag="VGP27_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV7854057.1"
                     /translation="MPKAKTHSGASKRFRRTGTGKIVREKANRRHLLEHKPSKRTRRL
                     EGRTVVSPNDIKRVKKLLNG"
     gene            complement(5131..5742)
                     /gene="infC"
                     /locus_tag="VGP27_22280"
     CDS             complement(5131..5742)
                     /gene="infC"
                     /locus_tag="VGP27_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV7854058.1"
                     /translation="MSTETRVNERIRVPEVRLIGPGGEQVGIVRIEDALRVAADADLD
                     LVEVAPDAKPPVCKIMDYGKFKYETAQKARESRKNQQQTVVKEQKLRPKIDPHDYETK
                     KGHVVRFLQAGSKVKVTIMFRGREQSRPELGYRLLQRLGADVADYGFVETSAKQDGRN
                     MTMVLAPHRGAKTRAKAAHDADAPAAQRASAQPSDEQTDTSKN"
     gene            5959..6348
                     /locus_tag="VGP27_22285"
     CDS             5959..6348
                     /locus_tag="VGP27_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEV7854059.1"
                     /translation="MTEVPASSDSTAQPSEPDSTAQPSVRELADIPAVEIITRAAVML
                     MSAAAEKLGLSAGHPDDSPHRDLDEARRLITALAGLVTASAEYLGPHAGPVRDGLKSL
                     QLAFRESSGAPDDPGQGPGEKYTGPVW"
BASE COUNT         1071 a         2140 c         2156 g         1175 t
ORIGIN      
        1 atcggcccgg cgtcgatgac gaacgcgctg acgaacagcg tgccgaggtg accgtggctg
       61 aggttgtgca gatttgtgct ggcgtgggtg atgacgcggt tctgcacctg gggtccgagg
      121 accgccaaag cggttgtcac acaggtcagg atcaccgcgt acacaacggt gacccgaatc
      181 cgggccagcg cccgcagcaa cccgaacagc atctcaccga ccactatgag tcggcgatgt
      241 ctccgttctc ctcgtcgcca cctggcaact ctctgcgagt tttcacgcgg tacaccgcca
      301 gatcggtggg caccccatcc tgcttcgatg agaagacctg cttgcgtatc cgcttggcgg
      361 tcaggccgag cgcttcgaac tccgcctcgg gaatgcggtc catcgtcgcc tgcgatacca
      421 ccaacccgcc ccttgcggca cgctccatca tgcgagcggc gatgttcacg tcgacgccga
      481 gccagtccgc cccgatccgc tgcggcctgc cagtgtgcac accggcacgt atacgtggtg
      541 tatagccctc cacctcaaca tgtttgaccg catcaaggct ggtgcgaact gcacggaccg
      601 cggtggcggg gtcggcgaaa accgccatgg tgccgtcgcc catcctcttg acgatgtgac
      661 cgcccgactc cagcagtggc ggctcgacga cctgggcgac ccggcgtaac agcttcagcg
      721 tggcgtcgtc accggcctcc aacgcccacg acgaaaaccc gaccaaatcc gtgaagacca
      781 gcgtcacctc gcgattggcg ggcttaccgg agacccgttc ggtgagcgcc tgccacacct
      841 gtagtcccgc gaggctcacc tcgcgggagg cggcgtcgcg ctccagcagt cggtcggccg
      901 cgcgggcggc ggcactcggc ccaccaacac cggcggcaga caacggatct ccaaaatcgg
      961 ggtcgccggg cagggcgcgg cgggctcggc ggatgaacgc gaccacccgg ggattgtgat
     1021 tgctgtcgcg cagcgaccgc caccgccccg ggccgcgctg ggccgacgcc gaaagatccg
     1081 gtgcctggtc gatctctccg atagcgccgg accgttcatc gaacgattcg acatccattg
     1141 cgcaaggtta gatgcccgtt ctttcagcgg gcaaacagct caacgtgcgg gactgctaag
     1201 tagctgacac cccgatatcc gcaagacgta cccgtcgcga cgcgcactga aatgatagac
     1261 aacgcgtgtt gtcaccctta tcgtggattt gtgacggtca gcgcgaggac gaagcgaacc
     1321 acaggggccg atccgctcgg cccggagtct ctgacctgga aatacttcgg cgaccttcgc
     1381 accggaatga tgggcgtgtg gatcggcgcg ttgcagacca tgtaccccga gctgggcgcc
     1441 ggcgtggagg agcattccgt cctgctgcgg gaaccgctgc aacgcgttgc ccgctcggtg
     1501 tacccgatca tgggcgtcgt ctacgacggt gaccgcgccg ggcagaccgg ccggcagatc
     1561 aagggcttcc acgccacgat caagggagtc gactccaccg gacggcgcta tcacgcattg
     1621 aaccccgaga ccttctactg ggcgcatgcc accttcttca tgttggtgat caaggtggcc
     1681 gagtacttct gtggcgggtt gaccgacgcc gagaagcgtc aactcttcga cgagcacgtg
     1741 cagtggtacg cgcagtacgg catgagcatg cgaccggtgc ccgaaacgtg ggaggagttc
     1801 tgcgagtact gggatcgggt ctgcagcgag gatcttgaga tcaaccgcgc gacgctcgac
     1861 atcctctcga tccggatccc caagccctgg ttcgtgctga tgccgactcc gatatgggac
     1921 cagctcttca agccgatggt ggccgggcag cgctggatcg ccgcgggatt gttcgacccc
     1981 gtcgtgcgag aaaaggccgg catgcgctgg accgccggcg acgaagttct gctgcggctg
     2041 ttcggcaaat tgatcgagat ggcgttcgtc gtagtgcccg acgagattcg cctgcacccg
     2101 cgcgcccttg ccgcctatcg ccgtgcatcc ggccgcatcg ccgatgacgc accgctggtc
     2161 gaggcgcccg cgttcatgtc gccgccgcga gaccgcaggg atctgcccat gcactacgtg
     2221 ccacgccgca agagcctgct ggatcgcgcc ggctcgctgg tgcacaccac gttctcgctg
     2281 gcaggcctgc gcccggcgcg gggccgcagc gccgccgcat gagctacgtg ttcctcgcgt
     2341 ccgcataggc tgacgggcat gctcgaatgg tctgatgtcg acatcgccgt acgcgacgct
     2401 gtgcgggatt tcgtagacaa agaggtccgc ccacacgtcg acgcgctgga aagcggcgac
     2461 atggagccct accccgtcat ccgcaagctg ttcaccgcct tcggcatcga cgcgctggcc
     2521 cgcgagtctt tggagaagcg gttggcccgg atgcgggaag gcgccgacaa atccgagggc
     2581 agcggcgcgt cgggcggcat gttcggcggc gacggcagtt ccgcaggcat gggcttcgtg
     2641 gtggtcagtg aactgtgccg ggtttgcatg ggtgtggtga ccggcatggg tgtcagcctc
     2701 ggcctgacgg tgccgaccat cgcgggccgc ggcaccctcg cgcagcagga acggtggctg
     2761 cccggtctgg tcacctacga gaaggtgggc gcgtgggcga tcaccgaacc cgactccggt
     2821 tccgatgcgt tcggcggcat gaagtcctac gtggtgcgtg acggtgacga ctacatcctc
     2881 aacgggcaga agacgttcat caccaacggg cccgacgccg atgtggtcgt ggtttacgcc
     2941 aagctggacg agggcgacgg tgccgacagg cgcgaccgca aggtactgac cttcgtgctg
     3001 gaccgcggca tggaaggctt cgtccagtcc aagccgttgc gcaagatggg aattcacagc
     3061 tcgcgcaccg gcgaactgtt cttcaacaac atgcggctcg gccgtgaccg gctgctgggc
     3121 gagaccgagg gcgaccccag cgattccgcc ggcgacgggc gggccagcgc gcggtcgaac
     3181 ttctccgccg aacgcatcgg cgttgccgcc atggcactcg gcgttatcga ggagtgcctc
     3241 aagctgtgcg tggactacgc caagacccgc aagctgtggg gtcaggagat cggccagttc
     3301 cagctgattc agctcaagct ggcgagcatg gaagtcgctc gaatcaacgt gcgcaacatg
     3361 ctgttccgcg tcatcgaggc ggcgcagacc gggacccaga tctcattgcc cgaggcgtcg
     3421 gcgatcaagt ggtactgctc gcaggcggcg accgacgtcg cgatggacgc tgtgcagctg
     3481 ttcggcggaa acggctacat gaccgaatac cgcgtcgagc aactcgcgcg tgacgccaaa
     3541 tcgctgatga tctacgcggg aagcaacgag gtgcagatca cccacgtcgc cagagggctg
     3601 ctcagcggct aaccggatct ggcttaccgg cgcgactgtg ccaatgtgtc ggccattgcg
     3661 ccgtgtctcc gaatgaatcc gcacagcgag ctagtggtgg cggtgcgcct tggcgctctc
     3721 gtagagacag atcgccgcgg cggaggcgac gttgaggctt tccgctgcac ccggcatggg
     3781 tatccggatc cgttctgccg ccatcgccgc cagctccggg ggcaggccgt gcgattcggg
     3841 cccgaacagc cacgcggtcg gcccggacag atcggtgtcg tccaggctcg tgctgccgtc
     3901 gacggtggtg gccagcacgc gcagacccgc ctcggagagc gcggaaaccg cggcccctgc
     3961 gtcggtctcg gacaccaccg gaaccgcgaa aatactgcct gcggaggcac gcagacactt
     4021 gccgttgtag gggtcaacgc tgtggccggc catgacgacc gcgtccgccc ccatcgcatc
     4081 gccgatccgg atcagcgtgc cggcattgcc gggctccgag atctcggcgg cgacggcgac
     4141 cagccgaggc tcgtcggcca acacgtcgtc cagggtgatt gacggaactt cacacacggc
     4201 gaccaggccc accggtgtca cggtgtccga caacgccttt gcagcgcgtt cggagacgat
     4261 gtggacttcg acgtcggtga gcagcgacgc aaagcgcgcc gatgcggcct cggtgacgaa
     4321 cacctcgatg accaggtttc gccgcagcgc cgcctctacg aggttcggtc cctcggcgag
     4381 gaagcgtgcg gcacggcgcc gtcccacatg gcggtgcagc ttgatcgctg ccgccacccg
     4441 gtctgagcgc tcggtgagcg tcaggcagcc tcgccggagt cggatgtcgc cgaccgaccg
     4501 gctccgccag gagcgttcac gtcctcgggc agcgcgcccc tggcgacgtc caccagcgcg
     4561 gtgaatgcgg ccgggtcgct gaccgcaatc tcggcgaggt tcttgcggtc gacctcgacg
     4621 cctgcggcct tgaggccctg gatgagccgg ttgtaggtga tgtcgttggc gcgggccgcg
     4681 gcattgatgc gggagatcca cagcttgcgg aattcgccct tgcgggcacg ccggtcacgg
     4741 tacgcatagt tcagagaatg cagctgctgc tctttggctt tgcgatacag ccgggaccgc
     4801 tgtccgcggt atcccttcga tgcctcgagt acggtccgcc gcttcttctg ggcgttgact
     4861 gcgcgcttca cgcgtgccat ggggtgttcc tattctcgtt cggtctaaaa ggtcagtggg
     4921 gcgcggaaac tacccgttga gcagtttctt gacgcgcttg atgtcgttgg gcgacaccac
     4981 ggtgcgcccc tcgaggcgac gggtgcgctt gctcggcttg tgttcgagca ggtgcctgcg
     5041 gttggccttc tcgcggacga tttttccggt gccggtgcgc cggaaacgct tggaagcgcc
     5101 gctatgggtt ttcgcctttg gcatgtgtcc tcagttcttt gatgtgtcgg tctgctcgtc
     5161 gctcggctga gcgcttgcgc gttgtgcagc cggtgcgtct gcatcatgcg ccgccttggc
     5221 gcgagtcttc gcgccgcggt gcggtgccag caccatcgtc atgttgcgcc cgtcctgctt
     5281 ggctgacgtc tcgacgaagc cgtagtcggc gacgtcggcg cccaggcgct gcaacagccg
     5341 gtagcccagt tcgggccgtg actgctcgcg tccgcggaac atgatcgtca ccttgacctt
     5401 cgaccccgct tgcaggaagc ggaccacgtg gccctttttg gtctcgtagt cgtgggggtc
     5461 gatcttgggg cgcagcttct gttccttgac gacggtctgc tgctggttct tgcgagactc
     5521 gcgcgccttc tgagccgtct cgtatttgaa cttgccgtag tccatgatct tgcagaccgg
     5581 cggctttgcg tccggggcta cttcgacaag gtcgagatcg gcgtccgcgg cgacgcggag
     5641 ggcgtcttcg atgcgcacga tgcctacctg ctcgccacct ggtccgatca ggcggacttc
     5701 aggtactcga atgcgctcgt tgacgcgggt ctcagtgctg atggggcctc ctatgttgtc
     5761 ctctaaggag cccaactacc agcacgctgc tcatctgaac agaaaagccc tgcataagca
     5821 gggcccgatg ccgaccagga caaccaccgc ggtggttgtg accggaccgc tgagccggtg
     5881 tggccaacgg tgggagcggg actccacttg ctgtccctga cgtacgccgg gacggtcgcg
     5941 catgccagtc taacaggcgt gaccgaggtt cctgcatcat ccgattcgac ggctcagccg
     6001 tccgagcccg attcgacggc tcagccgtcg gtccgcgaac tcgccgacat tccggcggtg
     6061 gagatcatca cccgcgccgc ggtgatgttg atgagcgccg cggcggagaa gctcggtctg
     6121 tcggccgggc atccggacga cagcccgcat cgcgatctcg acgaggcacg caggctgatc
     6181 accgcgctcg ccggattggt caccgcatcg gccgaatatc tgggcccgca cgcgggaccc
     6241 gtccgggacg ggctgaagag cctgcaactg gcgttccgcg aatccagcgg cgctccggat
     6301 gatccgggcc agggccccgg tgaaaaatac accggcccgg tctggtagct cctgctgttt
     6361 agctcctcgc gtgcgcgtca caccattttc tgagcttgac ggaatatcct cgcggcctat
     6421 gaccctcact agcccgccgc tggtacggcc cacctccaga tactcgtggg ttcccgcggc
     6481 cgcaggctgg accgtcggcg tcatcgcgac cttgtcgctg atcgcgagcc tctcaccggg
     6541 ca
//
LOCUS       DASYLX010000954         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3679345,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000954 DASYLX010000000
VERSION     DASYLX010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679345"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..573
                     /locus_tag="VGP27_22290"
     CDS             202..573
                     /locus_tag="VGP27_22290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV7854060.1"
                     /translation="MNLYLPILILGAIAAGFAVFSVVAALLGGPRRFNRAKLEAYECG
                     IEPMPELANPQAAGQRFPMKYYVTAMLFIVFDIEIVFLYPWAVAFDSLGVFALVEMLL
                     FMATVFVAYAYVWRRGGLNWD"
     gene            564..1118
                     /locus_tag="VGP27_22295"
     CDS             564..1118
                     /locus_tag="VGP27_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV7854061.1"
                     /translation="MGLEEKLPSGILLSTVEKLAGYVRKGSLWPATFGLACCAIEMMA
                     TAGPRFDISRFGMERFSATPRQADLMIVAGRVSQKMAPVLRQVYDQMAEPKWVLAMGV
                     CASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHDKIQEMPLGVNRE
                     EAVREAEKAALAIPPTIELKGLLR"
     gene            1232..>1550
                     /locus_tag="VGP27_22300"
     CDS             1232..>1550
                     /locus_tag="VGP27_22300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779122.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV7854062.1"
                     /translation="MFGPEGSGDTSGYGRLVREVAMPGSSPRPYGGYFDEVVDTLAAA
                     LGEQAFEAAVERVVVYRDQLTLEVRREHLRAVMQALRDDPGLRFELSLGVSGVHYPAD
                     SGRE"
BASE COUNT          247 a          499 c          529 g          275 t
ORIGIN      
        1 gtcgcgtcgc ttctgcgcgc cccggcgcag tagcaccttt ttgcgaaacc gcctgccgcc
       61 attggttctt cggcgcatgc caaagtgggc ggtttgcaac ctgaatcgat actaacccct
      121 tgttgcggca tgcatttcgg agatcgctag gctgtgggtc agctcacatc ggaaagccca
      181 cgagggagcg gtcacaactg catgaacctg tatctgccca tcctgatact cggcgcgatt
      241 gctgccgggt tcgccgtgtt ctcggtcgtt gctgcgctgc tcggcggacc acggcggttc
      301 aaccgtgcca agctcgaggc atacgagtgc ggcatcgagc cgatgccgga actggccaac
      361 ccgcaggccg ccggccagcg gttcccgatg aagtactacg tcaccgcgat gctcttcatc
      421 gtgttcgaca tcgagatcgt gttcctttac ccgtgggccg tcgcctttga cagcctcggg
      481 gtattcgcgc tggtcgagat gctgctgttc atggccaccg tgttcgtggc gtatgcctac
      541 gtctggcgca gaggaggctt gaattgggac tagaggaaaa gctgccgagc ggcattctgc
      601 tctcgacggt cgagaagctg gcgggctacg tccgcaaggg ctcgctgtgg cccgcgacgt
      661 tcggcctggc ctgctgcgcc atcgagatga tggccacggc gggcccgcga ttcgacatct
      721 cgcggttcgg catggagcgg ttctcggcaa cgcccaggca ggccgacctg atgatcgtgg
      781 ccgggcgggt gagccagaag atggcgccgg tgctgcggca ggtctacgac cagatggccg
      841 agcccaagtg ggtgctggcg atgggtgtgt gcgcgtcgtc gggcgggatg ttcaacaact
      901 acgccatcgt gcagggcgtt gaccacgtcg ttccggtgga catctacctg cccggctgcc
      961 cgccgcggcc cgagatgctg ctgcacgcga tcctgaaact gcacgacaag atccaggaga
     1021 tgccgctggg cgtcaaccgt gaggaggcgg tccgcgaagc cgagaaggcg gcgctggcga
     1081 tcccaccgac catcgagctc aaggggttgc tgcggtgatg cagtccaacg agcccggcca
     1141 gcacagcgag cacactgagg actccctcgc ccatcccgtc gacttcaccg acggcaaggg
     1201 cgagaaggag gtcgtcggcg tccgtcgcgg catgttcggc cccgaggggt ccggcgacac
     1261 ctccggctac gggcgactgg tccgcgaggt ggcgatgccc ggcagctcgc cgcgccccta
     1321 cggcggctac ttcgacgagg tcgtcgacac gctcgcggct gcgctcggcg aacaggcctt
     1381 cgaggcggcc gtcgagcggg tcgtcgtgta ccgcgaccag ctgaccctgg aagtccgacg
     1441 cgaacacctg cgcgcggtca tgcaggcgct tcgcgacgac ccgggcctgc ggttcgagct
     1501 gtccctcggc gtcagcggtg tgcactaccc ggccgacagc gggcgcgaac
//
LOCUS       DASYLX010000955         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1815428,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000955 DASYLX010000000
VERSION     DASYLX010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815428"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..193)
                     /locus_tag="VGP27_22305"
     CDS             complement(11..193)
                     /locus_tag="VGP27_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854063.1"
                     /translation="MSRGDHPERTPLYGVCLLAGAFLSLWLASSVSILWLSITIGVLA
                     VLASGAGFLMTFRDLG"
     gene            294..830
                     /locus_tag="VGP27_22310"
                     /pseudo
     CDS             294..830
                     /locus_tag="VGP27_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(900..2198)
                     /locus_tag="VGP27_22315"
     CDS             complement(900..2198)
                     /locus_tag="VGP27_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7854064.1"
                     /translation="MFSETCYARNGDLRVAYRASREGERDIVFVPNWFTCCEVLPEIP
                     SIQGWVEAMTTLGRLIFLDQPGTGASDPVTPGALPTLEQWGDSILAVLDDLGSREAVL
                     IAVDGAFAPATLFAATHPSRTSALIALDGYPDAAAVEHSEEFVAATVAMWGRGRLQHL
                     LNPDMPWNEEVRAAWARHERLAASPATVALMMPLLTELDIRALLPTVRVPTLVVQHTN
                     MMAVTGRYVADQIPAAEYVQLPGRNFYHFVEPDWRASFQEIARFLTGHRAEVADDRVL
                     ATLLFTDIVNSTRRAAEIGDREWHALLDAHDAVVRAQLARFRGREVNTSGDGFLATFD
                     GPQRAIRCALAIRDAVQALGIEVRAGLHTGECEIRGDDIGGIGVHIGARVSALAGPND
                     VLVSSTLRDLVIGSGLEFDDRGAHQLKGVPGDWHLFAVAG"
     gene            2341..2631
                     /gene="rpsF"
                     /locus_tag="VGP27_22320"
     CDS             2341..2631
                     /gene="rpsF"
                     /locus_tag="VGP27_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV7854065.1"
                     /translation="MRPYEIMVILDPTLDERTVSPSLETFLNVIRKDGGSVDKVDIWG
                     KRRLAYEIAKHAEGIYAIVDVKAEPATVSELDRQLNLNEAVLRTKVLRTDKH"
     gene            2736..3251
                     /locus_tag="VGP27_22325"
     CDS             2736..3251
                     /locus_tag="VGP27_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV7854066.1"
                     /translation="MAGDTIITVVGNLTADPELRFTPSGAAVANFTVASTPRIFDRQS
                     SEWKDGDALFMRCSIWREAAENVAESLTRGSRVIVQGRLKQRSYETREGEKRTVVELE
                     VDEIGPSLRYATAKVNKASRSGGGGGGFGSSGGGGGDRSGSRASEPKDDPWGSAPASG
                     SFSGGDDEPPF"
     gene            3288..>3520
                     /gene="rpsR"
                     /locus_tag="VGP27_22330"
     CDS             3288..>3520
                     /gene="rpsR"
                     /locus_tag="VGP27_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV7854067.1"
                     /translation="MAKATKRRPAPEKPVKTRKCVFCSKKGKGQTIDYKDTALLRTYI
                     SERGKIRARRVTGNCVQHQRDIAIAVKNAREVAL"
BASE COUNT          669 a         1151 c         1148 g          552 t
ORIGIN      
        1 gagcgcgtag ctagccgaga tcccggaagg tcatcaagaa cccggccccg gaagccagga
       61 ctgccagcac accgatcgtg atggacagcc acaggatcga caccgacgac gccagccaca
      121 gcgataagaa ggcgcccgcc agcagacaga cgccgtacaa gggcgtgcgt tctggatgat
      181 cgccacggga catgggcgtc gcgttcccgg cgcaccgccg cccgaaacat ccggactagc
      241 gagaccccgg ttcgcccgct gtcgccaact cgggctcctc ggccggcggc gcggtgaccg
      301 ccgtgctgtt cgtatcgtgt tgcgtcgcaa tcacgtacat cgcgctgacg gccaagcagc
      361 ggccgcggct catgcaggtg gcgttcttgg tggtggccac gttcctgttg acgaacaagg
      421 tgtggagccc gcagttctcg ctgtggctgg tgccgctggc ggtgctggcg ttgccgcacc
      481 ggcgaatcct gttggcgtgg atgacgatcg acgcgctggt gtgggtgccg cggatgctgt
      541 acctctacgg cgagcagaac cgcgggctgc ccgagcagtg gttcactatt actgttctgc
      601 ttcgtgacgt cgcggtgatt gcgctgtgcg cgttggtgat acagcagatc tatcgtcccg
      661 agctcgacct ggtgcgtaat cacggccagt tcgacgatcc aaccggaggc gtgttcgatg
      721 gcgcgccgga tcgtccgcct ggctggttgc cgaaacggtt acggcctgcg caaacggaaa
      781 ccgaaatcgc cattccggaa gagagaccgg cggctgttct caacgcttag gcgctgccgc
      841 gagcagacgc aaagtcaccc gacacgccgc gacgacacgc gcttgtgcgt ctgctcgcgc
      901 tagccggcga cggcgaacag atgccagtca ccgggaacgc ccttgagctg atgagcgccg
      961 cggtcgtcga actcgagccc cgacccgatc accagatcgc gcagcgtgct cgacaccaac
     1021 acatcgttcg gacctgcgag ggcactcacc cgcgcgccga tgtgcacgcc gatcccgccg
     1081 atgtcgtcgc cgcggatctc gcactctccg gtgtgcagcc cggcacgcac ctcgatgcct
     1141 aacgcctgca ccgcgtcgcg aatcgccagc gcacaacgga ttgcccgttg cgggccgtca
     1201 aatgtcgcaa gaaagccgtc gcccgacgtg ttgacttcgc gtccacgaaa gcgcgccaac
     1261 tgtgcgcgca ccacagcgtc gtgcgcgtcg agaagcgcat gccattctcg gtctccgatc
     1321 tccgctgccc gacgcgtcga attcacgatg tcggtgaaga gcaaggtggc cagaaccctg
     1381 tcgtcggcaa cctcggcccg gtgaccggtg agaaaccggg cgatttcctg aaaagacgcg
     1441 cgccagtccg gctcaacgaa gtggtagaag ttgcgccccg gcaactggac atactccgcg
     1501 gccggaattt ggtcagcgac gtatctaccc gttacagcca tcatgttggt gtgctggacg
     1561 acgagggttg gcacacggac tgtcggaagc agcgcccgaa tgtcgagttc ggtcagaagc
     1621 ggcatcatca gggcgaccgt cgccggacta gccgctaggc gctcgtgccg ggcccatgct
     1681 gcccgcacct cctcgttcca cggcatgtcc gggttcaaca ggtgctgcag tctcccgcgc
     1741 ccccacatgg cgaccgtggc cgcgacgaat tcctcgctat gttcgacggc cgcagcgtcc
     1801 gggtagccgt ccagcgcaat aagcgccgac gtacgggatg gatgcgttgc cgcgaacagc
     1861 gtcgccggcg cgaaagcgcc gtcgaccgcg atgaggaccg cttcgcggct gccgagatcg
     1921 tccaataccg cgaggatgct gtcgccccat tgctccaagg tcggcagcgc gcccggcgtc
     1981 actggatcgg acgctccggt gcccggctgg tcgagaaaga tgagccggcc gagcgtcgtc
     2041 atcgcctcga cccatccttg aatggatgga atctcgggaa ggacctcgca gcaggtgaac
     2101 cagttcggca cgaagacgat gtcgcgctcc ccttcacggg aagcgcggta ggcgacgcgc
     2161 aagtcaccgt tgcgggcgta gcacgtctcg gagaacacgg cggaagtgta tgcatcccga
     2221 gcagtgcgat ttcgcagatc agcaggggtt cctgtaacct gggccagttg ccgacgcagg
     2281 cgaccctcct gccacggaca ggccgtggcc gactagccca taggaggtga tgaggtcctc
     2341 atgcgtccat acgaaatcat ggtcatcctc gaccccaccc ttgacgagcg caccgtcagc
     2401 ccctcgctgg agacgttcct caacgtcatc cgcaaggacg gcggctcggt cgacaaggtc
     2461 gacatctggg gcaagcgccg gctggcatac gagatcgcca agcatgccga gggcatctac
     2521 gccatcgtcg atgtgaaggc ggagcccgcc accgtgtccg agctcgaccg tcagctcaac
     2581 ctgaacgagg ccgtgctgcg gaccaaggtg ctgcggacgg acaagcacta aaggctgccg
     2641 gtagtcgggg tggctgcgta ggctcgccca cgacaaccac cgccctgcga tgagcgctcg
     2701 cgcggagagc ggacactact gcccaggagg acttcgtggc tggtgacacg atcatcaccg
     2761 tcgtcgggaa tctgaccgcc gaccccgaac tgcggttcac cccgtccggc gctgccgtcg
     2821 ccaacttcac ggtggcgtcc actccgcgga tcttcgaccg gcagagcagt gagtggaaag
     2881 acggagacgc actgttcatg cggtgcagca tctggcgcga ggccgccgag aacgtggccg
     2941 agagcctgac ccgcggttcg cgggtgatcg tgcagggccg gctcaagcag cgttcctacg
     3001 aaacccgtga aggcgagaag cgcaccgtcg tcgaactcga ggtcgacgag atcgggccgt
     3061 cgttgcgcta tgcgaccgcc aaggtgaaca aggccagccg cagcggcggc ggtggcggcg
     3121 gattcgggag cagcggcggt ggcggaggcg accgcagtgg ctctcgcgcc tccgagccaa
     3181 aggacgaccc atggggcagc gctccggcgt ccggatcgtt ctccggcggc gacgacgaac
     3241 cgccctttta gaaccaacaa aatagcaatt gagaaagaga tagagacatg gccaaggcca
     3301 ccaagcggcg tccggctcca gagaagccgg tcaagacccg caagtgcgtg ttctgctcga
     3361 agaaggggaa ggggcagacc atcgactaca aggacaccgc tctgctgcgg acctacatca
     3421 gcgagcgagg caagatccgt gcccgtcgcg tcaccggcaa ctgcgtgcag caccagcgcg
     3481 acatcgctat cgcggtgaag aacgcccgcg aggtcgcgct
//
LOCUS       DASYLX010000956         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1705325,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000956 DASYLX010000000
VERSION     DASYLX010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705325"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP27_22335"
     CDS             complement(<1..351)
                     /locus_tag="VGP27_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEV7854068.1"
                     /translation="MPDAWGRERPQPVAVGPPRRRMSRADRMREARRRRRHRLVRSVT
                     LCVFIVIVIGAVFLGSRLWHNVFGGGSDYSGEGRSDVVIQVHEGDSTTTIGKTLEDQK
                     VVATSRAFVNAAQDN"
     gene            complement(344..841)
                     /gene="ruvX"
                     /locus_tag="VGP27_22340"
     CDS             complement(344..841)
                     /gene="ruvX"
                     /locus_tag="VGP27_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV7854069.1"
                     /translation="MPNPENRPPDRPGGDDPGRGRRMGIDVGSVRIGVAVSDPDCILA
                     TPVETVRRDRTDRHMRRLTQLVNELDAVEVVVGLPRTLADRTGPSAEDAISLAEDLAR
                     RVSPTPVRMADERLTTVSAQRSLREAGVRARGQKAMIDQAAAVAILQSWLDQRRVALN
                     EVGNA"
     gene            complement(846..>1668)
                     /locus_tag="VGP27_22345"
     CDS             complement(846..>1668)
                     /locus_tag="VGP27_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEV7854070.1"
                     /translation="QRTQIEEVTNEAVEADFEVHSFTTDLEKAKAMGAMALFGEAYPD
                     EVRVVEIGGPFSLELCGGTHVRNSAQIGPVTILGESSVGSGVRRVEAYVGLDSFRHLA
                     KERALMAGLASSLKVPSEEVPARVTQLVDRLKSAEKELDKMRLAGARAAAVNAAAGAE
                     RIGRVRVVAQRMAGGTSAGDLRSLVGDIRGKIGSEPAVIALIAEGDNDAVPFVVAVNP
                     AAQDLGLKANELVKELGAAVNGRGGGKADLAQGSGKGAAGIDAALAALRAEIGRS"
BASE COUNT          243 a          612 c          526 g          287 t
ORIGIN      
        1 gttgtcctga gcggcgttga cgaatgcccg cgatgtcgca acaaccttct ggtcttcgag
       61 cgtcttgccg atggtcgtgg tcgagtcgcc ctcgtggact tggatgacga cgtccgacct
      121 gccttcgccg gaatagtcgg accctccccc gaagacgttg tgccacaacc gggagccgag
      181 gaaaacggcg ccgattacga tgacgatgaa cacgcagagc gtgacactgc ggaccaaccg
      241 atgtctgcgg cgacggcgag cttcgcgcat ccggtcggcg cggctcatgc gacgccgcgg
      301 cggccccacc gctaccggct gcgggcgttc gcgcccccag gcgtcaggca ttgccgacct
      361 cgttgagtgc gacccggcgc tgatccagcc agctctgcag tattgcgacc gctgcggcct
      421 ggtcgatcat cgccttctgc cccctggccc ggacaccggc ttcccgcagt gagcgctgcg
      481 cgctgacagt ggtcagccgt tcgtcggcca tccgcaccgg agtcggcgaa acgcgacgcg
      541 ccagatcctc cgcgagagag atggcgtctt cggccgacgg accggtccga tcggcgagtg
      601 tgcgcggcag tccgaccacc acttcaacag cgtccaattc gttgacgagt tgggtcagcc
      661 gtcgcatatg gcggtccgtt ctgtcacggc ggacggtctc caccggtgtg gcgaggatgc
      721 agtcagggtc gctcaccgcg acgccgatcc gaacacttcc cacgtcgata cccatccgtc
      781 gtccccgtcc gggatcgtcg cctccgggcc gatccggcgg acggttttct gggttcggca
      841 cgtggttagc tccggcctat ctccgcgcgc agcgcggcga gcgcggcgtc gatgcctgcg
      901 gctcccttgc cggagccctg tgccaggtcc gccttgccgc cgccccggcc gttcaccgcc
      961 gcacccagct ccttgaccag ctcgttggcc ttcagcccga ggtcctgggc ggccgggttg
     1021 acggccacca cgaacggcac ggcgtcgttg tcgccctccg cgatcagcgc gatcaccgca
     1081 ggctcggagc cgatcttgcc gcggatgtcg ccgacgagac tgcgcaggtc ccctgccgat
     1141 gtgccgccgg ccatccgctg cgccacgaca cggacccgac cgatccgctc ggctcccgct
     1201 gcggcattca ctgcagctgc cctcgcaccg gccagccgca tcttgtcgag ttccttctcg
     1261 gcgctcttca atcgatccac cagctgggtc acccgtgcgg gcacctcttc cgagggcacc
     1321 ttcagcgacg acgccaggcc cgccatcaac gctcgttcct tggcgagatg gcggaacgag
     1381 tcgaggccga cgtaggcctc cactcgccgc acacccgagc cgaccgacga ttcaccgagg
     1441 atcgtgacgg gaccgatctg cgcggaattg cggacgtgcg tgccgccgca cagctcgagc
     1501 gagaacgggc cgccgatctc gaccacccgc acctcgtcgg ggtaggcctc tccgaacagc
     1561 gccatcgcgc ccatcgcctt ggccttctcg aggtcggtgg tgaaactgtg cacctcgaag
     1621 tccgcctcga cggcctcgtt ggtgacctct tcgatctggg tgcgctga
//
LOCUS       DASYLX010000957         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3241005,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000957 DASYLX010000000
VERSION     DASYLX010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241005"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGP27_22350"
     CDS             <1..270
                     /locus_tag="VGP27_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7854071.1"
                     /translation="HSAAYSGFEGTDESGTPLVDWLRQRAIDEVDIVGIATDYCVLAT
                     ATDAAAEGFTTRVLLDLTAGVAGESSAKAVEDMRAQGVEVIGSAA"
     gene            482..>1701
                     /locus_tag="VGP27_22355"
     CDS             482..>1701
                     /locus_tag="VGP27_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV7854072.1"
                     /translation="MTRAATPQSAAPEIWPGKAYPLGATFDGFGTNFAVFSEEAEKVE
                     LCLFDPDGVETRVTLPEVDGFVWHAFIPGIQPGQRYGYRVHGPYDPSAGQRCNPNKLV
                     LCPYSKAIDGTFDWNQSLFGYNFGDPDSRNDDDSAASMPKSVVINPYFDWGVDRPPGH
                     EYADSVIYEAHVKGLTQTHPGIPENIRGTYAAIGHPAMIEHMKSLGVTAIELMPVHHF
                     ANDSTLIEKGLSNYWGYNTIGFFAPDPKYSSNPAPGGQVQEFKAMVRALHEANIEVIL
                     DVVYNHTAEGNHMGPTLSMRGIDNAAYYRLVEDDKRYYMDYTGTGNSLNVGHPHSLQL
                     IMDSLRYWVTEMHVDGFRFDLASTLAREFYDVDKLSTFFELVQQDPTVSQVKLIAEPW
                     DVGPGGYQVGGFPP"
BASE COUNT          312 a          555 c          544 g          290 t
ORIGIN      
        1 cactcggcgg catacagcgg attcgagggc acggacgaat ccggcacgcc gttggtggat
       61 tggctccggc agcgcgcaat cgacgaggtc gacatcgtgg ggatagccac cgactactgc
      121 gtgctggcta ccgcgacgga cgcggccgcc gaaggcttca ccacccgtgt gctgctcgac
      181 ctgaccgccg gcgtcgcagg agagtccagc gccaaggcgg tcgaggacat gcgcgcccaa
      241 ggcgtcgagg tgatcggctc agcggcctga cgcacacatc gatgcccgcg gccggttcag
      301 cgtgacggtt gccacatcgc ggtcgatgtc gacgagcaga gtccggtaag agggcaggtc
      361 ggcactgttt ggcacgcgga ctgcctcccg tcggtcacgg tcgacgactt tggttgtgat
      421 gctcccccac tgggcatccc cgtatggtct cgttggtccc gtcaattttg gagtgtgttc
      481 gttgacccga gctgctaccc cgcagtccgc cgcgccggag atctggcctg gcaaggcgta
      541 tccccttggc gccaccttcg atgggttcgg taccaacttc gcggtgttca gcgaggaggc
      601 ggagaaggtc gaactgtgcc ttttcgaccc cgacggcgtc gagacccggg tgacgctgcc
      661 cgaagtcgac gggttcgtct ggcacgcgtt catcccgggc attcagccgg gtcagcggta
      721 cggctaccga gtgcacggcc catacgatcc gtcggccggt cagcggtgca atccgaacaa
      781 gctggtgctc tgcccgtact cgaaggcgat cgacggcact ttcgactgga accagtcgct
      841 gttcgggtac aacttcgggg atccggacag tcgcaacgac gacgactcgg cggccagcat
      901 gcccaagtcg gtggtcatca acccgtactt cgactgggga gtggaccggc cgccgggtca
      961 cgagtacgcc gactccgtga tctatgaggc gcacgtgaag ggcctcaccc agacgcaccc
     1021 cggtatcccg gagaacattc gcggcaccta cgccgcgatc ggacacccgg cgatgatcga
     1081 gcacatgaag tcgctcgggg tgacggccat cgagctcatg ccggtacacc acttcgcgaa
     1141 cgactccacg ctgatcgaaa aggggctgtc caactactgg ggttacaaca ccatcgggtt
     1201 ctttgctccc gaccccaagt acagcagcaa ccccgcgccg ggcgggcagg tgcaggagtt
     1261 caaggcgatg gtgcgcgcgc tgcacgaggc caacatcgag gtgatcctcg acgttgtcta
     1321 caaccacacc gccgagggca accacatggg cccgacgctg tccatgcgcg gcatcgacaa
     1381 cgccgcctac taccgcctcg tcgaggacga caagcgctat tacatggact acaccggcac
     1441 aggtaacagc ctcaacgtcg gacacccgca ctcgttgcag ctgatcatgg actcgctgcg
     1501 ctactgggtg accgagatgc acgtcgacgg cttccgattc gacctggcct cgacgctggc
     1561 ccgcgagttc tatgacgtcg acaagctgtc gacgttcttc gaactggtgc aacaggatcc
     1621 gacggtcagc caggtgaagc tgatcgccga gccgtgggac gtcgggcccg gcggctatca
     1681 ggtcggcggc ttcccgccgc a
//
LOCUS       DASYLX010000958         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2145969,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000958 DASYLX010000000
VERSION     DASYLX010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145969"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VGP27_22360"
     CDS             <1..1676
                     /locus_tag="VGP27_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854073.1"
                     /translation="GALVAVVVVVAAFILWRFFGDALSDRSSIASARCVDGQKAVAVI
                     ADPSIADEISTLAKKFNETANPVGDSCVEMDVKAADSDQVVNGFVGTWPAELGERPAL
                     WIPGSSISQARLEAVAGEETVDDSRSLVSSPVVLAIRPQLKSALAQQNWGTFPGLQSN
                     PTSLNGLNLPGWGSLRLALPLSGDSDASYLAAEAVAAASAPAGAPPSAGTGAVNTLMS
                     GQPKLADNKASTAMDALLKANNPAAAPVHAVVTTEQQLYQRGTSLSDAKNVVASWIPP
                     GPSATADYPTVLLKGTWLSQEQVTAASEFSRYLRKPEQLAELAKLGFRTDGGETPKSD
                     VTNFAPIPAPLSVGDSTMRATLANALTTPADNPAVTIMLDQSMSAQEGGKTRLANVVA
                     ALKAQLQTMPPTSAVGLWTFDGVEGRSEVATGPLSDPVDGKPRSAALTATLDGQTASG
                     GGAVSFTTLRIIYDDALAKFRQGQTNSLLVITAGPHTDQSLDSSGLQDYVRRTFDPAR
                     PVAINVIDFGGDPDRAAWEAVAQISGGDYQNLSTSASPDLSSAVAKFLG"
     gene            complement(1744..>2043)
                     /locus_tag="VGP27_22365"
     CDS             complement(1744..>2043)
                     /locus_tag="VGP27_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854074.1"
                     /translation="VGTEILPGVYQSAGPVEGSACYWKRSNNDGTVANAMTKKPQTVE
                     IAPGDTTFKTSQCQEWQKTDAAPPPPPNPGDLLGQLGSLVGPAVLGPSGGASSGG"
BASE COUNT          325 a          706 c          684 g          328 t
ORIGIN      
        1 tcggcgcact ggtcgcggtc gtcgtcgtgg tggccgcctt catcctgtgg cgcttcttcg
       61 gtgacgcgtt gtcggaccgg tccagcatcg cctccgcccg atgcgtcgac ggtcagaaag
      121 ccgtggccgt catcgccgat ccgtccatcg ccgacgaaat ctccacgctg gccaagaagt
      181 tcaacgaaac agccaacccg gtcggtgaca gctgcgtcga gatggacgtc aaggcggcgg
      241 actccgatca ggtggtgaac ggcttcgtcg gtacctggcc tgccgagctc ggcgaacgtc
      301 cggcgctgtg gattcccggc agttcgatct cccaggcccg cctcgaggcg gtggcaggcg
      361 aggaaaccgt cgacgacagc cgctcgctgg tcagctcacc cgtcgtgctg gcgatccgtc
      421 ctcagctcaa gagtgcgctc gcgcagcaga actggggtac cttcccgggt ctgcagagca
      481 accccacctc gttgaacggc ttgaatctgc ccggctgggg ttcgttgcgt ctcgcgctgc
      541 cgctgagcgg cgacagtgac gcgtcctacc tcgccgccga ggctgtcgct gcggcgtccg
      601 caccggccgg agccccgccg agcgcaggca ccggcgcggt caacaccctg atgtcgggcc
      661 agcccaagct cgccgataac aaagcgtcga ccgcgatgga cgcactgctg aaggcgaaca
      721 atcccgccgc ggcgccggtg catgccgtcg tcaccaccga gcagcagctg tatcaacgcg
      781 gtacctcgct ctcggacgcg aaaaacgttg tggcctcctg gattccgccg ggcccgagcg
      841 cgacggccga ctacccgacg gtgcttctca agggcacgtg gctgtcacag gaacaggtga
      901 ccgctgccag tgagttctcc cgctacctcc gcaagcccga acaactcgcc gagctggcca
      961 agttgggctt ccgcaccgac ggcggcgaga cgccgaagag cgacgtcacg aacttcgccc
     1021 cgatcccggc tcccctgtcg gtcggcgact cgaccatgcg ggccaccctc gccaacgcgc
     1081 tgaccacacc ggcggacaac ccagccgtca cgatcatgct cgaccagtcg atgagcgcgc
     1141 aggagggcgg caagacccgg ctcgccaacg tggtcgcggc gctgaaggcg cagttgcaga
     1201 cgatgccgcc gacctcggcg gtcgggctgt ggacgttcga cggtgtcgag gggcggtcag
     1261 aggtggcgac cggtcccctg tccgatcctg ttgacggcaa gccacggtcg gcggccttga
     1321 ctgcgaccct ggacgggcag acggcgtcgg gcggcggcgc cgtatcgttc acgacgctgc
     1381 ggatcatcta cgacgatgcg ctggcgaagt tccgtcaggg gcagacgaat tcgcttctgg
     1441 tgatcacggc gggtccgcat accgatcaaa gcctcgacag cagcggcctg caggattacg
     1501 tgcgccggac gttcgatccc gcacggccgg tcgcgatcaa cgtgatcgac ttcgggggcg
     1561 acccggaccg cgcggcgtgg gaggcggtcg cccagatcag cggtggcgac taccagaacc
     1621 tgtcgacatc ggcatcgccg gatctgtctt cggcggtcgc caagttcctc ggctagcgcg
     1681 ccggcgcagc tcaaaaggta aggggcccgc cgggtcccgc gtcagtcacc cgcgagcggg
     1741 gtatcagccg ccgctcgacg cgccgccgct cggcccgaga acggccggac cgaccaggga
     1801 gcctagctgg cccagcaggt caccgggatt cggcgggggc ggcggtgcgg cgtcggtctt
     1861 ctgccactcc tggcattggc tggtcttgaa tgtcgtatcg cccggcgcga tctccaccgt
     1921 ctgcggtttc ttggtcatcg cattggcgac ggtgccgtcg ttgttggacc gcttccaata
     1981 gcaagcgctg ccctcgaccg gaccggctga ctggtacacg ccgggcagga tctcggttcc
     2041 gac
//
LOCUS       DASYLX010000959         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_498101,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000959 DASYLX010000000
VERSION     DASYLX010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498101"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VGP27_22370"
     CDS             complement(<1..252)
                     /locus_tag="VGP27_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV7854075.1"
                     /translation="MVAPVASRPAAPVSRSETAAPPGPLVSATVAILVAATCIPLGYV
                     VWGAVSVGWTRAYELVVRPRVGELLFNTVALVVVTVPLCV"
     gene            complement(253..1296)
                     /locus_tag="VGP27_22375"
     CDS             complement(253..1296)
                     /locus_tag="VGP27_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEV7854076.1"
                     /translation="MRHGAREEHGKARWSRIAAFVSILAVVLGMTACSKSGDGDGLLV
                     YNAQHESLTKEWIDAFTKETGIKVTYRQGGDTELGNQLVAEGDASPADVFLTENSPAM
                     AAVEKAGLFADVNPDTLAQVPAQYRPATGKWTGVAARSTVFVYNKAKLAPDQLPKSMA
                     DLAQPAWKGRWGGAPAKADFQAIVAAYLALRGEAATAQWLAGMKANAKIYNDNIATMK
                     AVNAGEVDGGIIYHYYWFRDQANTKEGSGNTALHYFKNSDPGAFVSLSGGGVLKSSKK
                     TDQAQQFIKFVTAKAGQEVLEKGASFEYPVASGVPGNSALPPLDSLQAPKVDPSTLDA
                     QKVADLMTKAGLL"
     gene            1561..1947
                     /locus_tag="VGP27_22380"
     CDS             1561..1947
                     /locus_tag="VGP27_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7854077.1"
                     /translation="MTLSATTCRAVIGAFSAFAVAAAAAAIGGLPTADAAPCSASGLA
                     STASGVMADAGTYLAAHPGADDVLTKAGSQPTDEAKANVQSYFMGHPGEFLDLKGIAK
                     PLTDLRAQCGVAVSPGQLAALVESFQ"
     gene            complement(1957..>2544)
                     /locus_tag="VGP27_22385"
     CDS             complement(1957..>2544)
                     /locus_tag="VGP27_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEV7854078.1"
                     /translation="LFALGLWRVGEAIGGSKTGERSGRQQDDSPAWKRVKPLGLAIVN
                     FAIAFSAARFAMGTGKSSGQQNAGMSAQLMQSGWGKALLIAVGIGVAVVGGYHVYKGA
                     TKKFLKDLRVPGGTGITAVGVTGYVAKGLVLGGAGLLVVIATLQADPSKAAGLDAAVK
                     TLGQAPFGKILLILAALGIAAFGAYSFVRSRHGRM"
BASE COUNT          424 a          860 c          827 g          433 t
ORIGIN      
        1 cacacacaac ggcacggtga cgacgaccag cgcgacggtg ttgaaaagca gctcaccgac
       61 ccgcgggcgg accaccaact cgtaggcgcg ggtccacccc acagagacgg cgccccacac
      121 gacgtacccg agcgggatgc acgtcgctgc caccagaatc gcgacggtcg ccgagaccag
      181 cgggccgggc ggggccgccg tctccgaccg cgaaaccggt gccgcggggc gcgaggctac
      241 aggagcgacc acctacagca agcctgcctt cgtcatcaga tcggctacct tctgggcgtc
      301 cagcgtcgag gggtcgacct tgggcgcctg tagcgaatcg agcggcggca gagccgaatt
      361 cccgggaaca ccgcttgcca cggggtactc gaaagaggcg cccttctcga gcacttcctg
      421 tccggccttg gcggtgacga acttgatgaa ctgctgcgcc tggtcggtct tcttgctgga
      481 tttcagcaca ccgccgccgg acaggctgac gaacgcgcca gggtcggagt tcttgaagta
      541 gtgcaacgcc gtattgccgc tgccctcttt ggtattggcc tgatcgcgga accagtaata
      601 gtggtagatg atgccgccgt cgacctcgcc ggcgttgacc gccttcatcg tggcgatgtt
      661 gtcgttgtag atcttcgcgt tggccttcat gcccgccaac cactgcgcgg tggccgcctc
      721 gcccctgagc gcaaggtagg ccgccacgat ggcctggaag tccgccttgg cgggtgcgcc
      781 gccccatcgg cccttccacg cgggctgcgc aaggtccgcc atggatttcg gaagttgatc
      841 gggtgccaac ttggccttgt tgtagacgaa cacggtgctg cgcgcggcga cgccggtcca
      901 cttgccggtg gccgggcggt actgcgccgg aacctgggcg agggtgtcgg ggttcacgtc
      961 ggcgaacagg ccggccttct cgactgcggc catggcgggg gagttctcgg tcaggaaaac
     1021 atcggcgggc gaggcatcgc cctcggcgac cagttgattg ccgagttcgg tgtcgccgcc
     1081 ctggcggtag gtgaccttga tgccggtctc cttggtgaag gcgtcgatcc actccttggt
     1141 cagggactcg tgctgggcgt tgtagacgag cagtccatcg ccgtcgcccg acttcgagca
     1201 cgcggtcatt ccaagcacaa cggcaaggat cgacacgaag gcggcgatcc ggctccaacg
     1261 ggcttttcca tgctcttcgc gtgcgccgtg tcgcatccgg ccggtccttc ccgcgcctgc
     1321 cggcgcttta gctgagggtt acctaaatga acataccctt cccagtcccg tcaaatggtt
     1381 gcccccgggg aaggaacaag gcgcattcat aggctatgag gcgactggca taccggcccc
     1441 ctcccgtcag gcgcttccgc cgtagcgggg cgcgttcgag attcccttga aatactgcgg
     1501 gtacgtaggt tttcaaatcc ggacagatca cgcacgaccg acgacaccga ggacacatcg
     1561 atgacactgt cagctactac ctgccgcgct gtgatcggcg ccttctccgc ctttgcggtg
     1621 gcggccgcag cggcggccat cggcgggctg ccgacggccg acgccgcacc gtgttcggcc
     1681 agcggcttgg cctccacggc cagcggggtg atggccgacg ccggcacgta cctggccgct
     1741 caccctggtg ccgacgatgt gctgaccaag gcggggtcgc agccgacgga cgaggcgaag
     1801 gccaacgtgc agtcgtactt catgggccac cctggcgaat tcctggacct gaagggcatc
     1861 gccaaaccgt tgaccgacct gcgcgcccag tgcggtgtcg cggtgtcacc gggacagctg
     1921 gccgcgctcg tcgagtcgtt ccagtaggcc ggtgcgctac atccggccgt gccggctgcg
     1981 cacgaagctg tacgcaccga aggccgcgat cccgagcgcg gccagaatca acaggatctt
     2041 gccgaacggc gcctgaccga gcgttttcac cgcggcatcg aggccggccg ctttcgacgg
     2101 atccgcctgc agggtggcaa tgacgaccag caagccggcg ccaccgagaa ccagaccctt
     2161 ggccacgtaa ccggtcacgc cgacggcggt gatcccggtg ccgccgggca cccgcagatc
     2221 cttgaggaac ttcttcgtcg cgcccttgta gacgtgatac ccgccgacga cagcgacgcc
     2281 gattccgact gctatcagca gcgccttgcc ccagccggac tgcatcagct gagcggacat
     2341 cccggcgttc tgctgaccgc tcgactttcc ggtgcccatc gcgaatcgcg ccgccgagaa
     2401 tgcgatcgcg aagttgacga tggccaaccc gagcggcttg acgcgcttcc acgcagggga
     2461 gtcgtcctgc tgtcgtcctg atctctctcc ggtcttggag ccgccgatgg cctcaccgac
     2521 gcgccacagg ccgagggcga acag
//
LOCUS       DASYLX010000960        20586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2253108,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000960 DASYLX010000000
VERSION     DASYLX010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253108"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..9880)
                     /locus_tag="VGP27_22390"
     CDS             complement(323..9880)
                     /locus_tag="VGP27_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV7854079.1"
                     /translation="MTADATRLLSIDLFDDDEHEQLDEWANRAVLDMPVGASASIPEL
                     FAQHVTRSPEAVAVSFDGHSMTYRQLDEAADRLAHRLAAEGAGPGQCVAVLLNRSIEA
                     VVAVLAVLKAGAAYLPIDPAHPAVRIDFVLEDSGPVAALTTEALRSRLDGRNVAVVAV
                     EDAPAETRPSTGMTAPGPEDIAYLIYTSGTTGVAKGVAISHRNVAQLLESLDAGLPRP
                     GVWALCHSLSFDVSAWEILAPLVSGGRVVVVTEAVMSSPDDFHDLLVAERVTVLTNTP
                     SAMGVLSSEGLESLALVMAGEACPSEVVDRWAPGRVMINAYGPTETTMCVAISAPLEP
                     GTGAPPIGSPVPGAALFVLDKWLRTMPVGVVGELYVAGNGVGVGYVGRAGLTGSRFVA
                     CPFGRPGLRMYRTGDLVRWSADGQLQYLGRVDEQVKIRGYRIELGEVQSALAAFEGVE
                     QAAVIAREDRPGDKRLVGYVTGDPDPIRARSVLAERLPAYMVPAAVVVLEALPLTING
                     KLDVRALPAPEFQETAKRYRAPANPTEEILAEIYSQVLGVKRVGADDSFFDLGGDSIL
                     AMRAIAAVNRSLDAGLSVRVLFEAPSVAELASRVGVDGDVSVPLVAVERPAVVPLSFA
                     QNRLWFLDQLHGPSPIYNMPVALRLSGLLNTEALQVALADVVSRHETLRTLFPVVDGT
                     PQQSVLSADHIDFGWDIVDATAWSANQLSEAVEEIAGYAFDLAAEIPLQARLFHVNDT
                     EHVLVAVVHHIAADGLSASPLVRDLGAAYLARQQGKAPEWAPLPVQYVDYTLWQRGHL
                     GELEDSDSRIAGQVAYWQDALAGLPERLELPTDRPYPTVADYRGAHLAVEWPAALQQQ
                     IHATARTHNATSFMVVQAALAVLLSRLSANSDVAVGFPIAGRSDAALDDLVGFFVNTM
                     VLRIDLSGNPTTSELLAQVRQRSLAAFEHQDVPFEVLVDRLNPARSMAHHPLVQVLLG
                     WQNLAGAVNEGAGGLALGDVKASPLSAETHTARVDLTFSLAERWTDDGEPAGIGGLVE
                     YRTDVFDAASIETLIERLARVLAAMTVDPGRQLSSVDVLDAGELARLDGWSNRAVLAK
                     PAPTGVSIPVLFAAQAARTPQAVAVTFDDRSMTYRELDEASNRLAHLLIGQGARPGRC
                     VALLIPRSAEAVVAIMAVLKTGAAYLPMDPAHPDARIGFMLEDAAPIAAVTTTAELAM
                     RLHGSNAAIIGVNDAAVAAQPCTALPAPAPDDIAYIIYTSGTTGVPKGVAIPHRNVTQ
                     LLDSLDGDLELAGQVWTQSHSLAFDFSVWEIFGALLRGGRVVVISESAARSPEDFHAL
                     MVSERVGMLSRTPSAFYALQTADALHSERGQQLELQTVVFGGEALEPQRLRMWLDNHP
                     RSPRMINMYGITETTVHASFREIVDGDTDRNVSPIGVPLAHLGFFVLDKWLRPVPRGV
                     IGELYVAGGGLAIGYVGRPSLSSTRFVACPFGAPGARMYRTGDVVRWDADGQLQYLGR
                     ADKQVKIRGYRIELGEIVAALTDLDGVDQAAVIVREDHPGDKRLVAYVTGAADPAGMR
                     AVLAERLPNYMVPAAVLVLNDLPLTVNGKIDERALPAPEYQDFDRYRAPSNAVEELLA
                     DIYAQVLGVQRVGVDDSFFDLGGDSISAMRVIAAANKSLDAGLSVRSMFESQTVARLA
                     SRVGGESAGFEPLVPVERPATVPLSFAQNRLWFLDKLHGPSPVYNMPVALRLNGPLDA
                     QALGQALSDVVGRHESLRTRFVAHEGIPQQLVMPADSVDFGWHVVDATGWAADQLEEA
                     IGAAVRHTFDLTAEIPLRAQLFRVASDEHVLVAVVHHIAADGLSIVPLVGDLGVAYEA
                     RRQGRSPGWAPLAVQYVDFTLWQREQLGELDDSNSRIAAQLAYWMDALAGMPEQLQLP
                     TDRPYPPVADYRGSSVAVDWPVELQQRVREVARAHNATSFMVMQAALAVLLAKISASS
                     DVAVGFPIAGRSDPALDELVGFFVNTLVLRVDLDGDPTIADVLAQVRQRSLAAYEHQD
                     VPFEVLVDRLNPTRSLNRHPLVQVMLAWQNFAGQDFDSVLGDVRVSPLSADTDTARVD
                     LTFSLAELWGADGEPGGIGGAVEFRTDVFDAGSIETLIERLARVLAAMTADPGRRLSS
                     VDVVDADELVRLHEWGNRAMLTESTPAADSIPVLFEAQLGRTPNAVAITCEGRSMTYR
                     ELDEASNRLAHLLGEHGAGPGECVALLVPRSAEAVVAILAVLKTGAAYLPMDPAHPDA
                     RIGFMLGDSAPVAAVTTAELAERLVEFGIQIIRVNDLRIDVQPSTALPAPAPDDIAYI
                     IYTSGTTGVPKGVAISHSNATQLLETLPVDLPRAKVWSQWHSYAFDVSVWDIFAPLLA
                     GGRLVVVSEAAAASPDDLHALLVAEQVDVLSQTPSAVGMLSPEGLDTMALVVAGEACP
                     TDLMDRWAHGRTMINAYGPTEATVFSAMSAPLKAGSRVVPIGAPVPGAALFVLDKWLR
                     PVPTGVVGELYVAGAGVGCGYVRREGLTAARFVACPFGNAGQRMYRTGDLVRWGADGQ
                     LQYMGRADEQVKIRGYRIELGEIQAALVGLDGVEQAAVIAREDRPGDKRLVAYVTGTA
                     DPAWTRSVLAERLPRYMVPAAVMALETLPLTVNGKLDTHTLPAPKYDDADRYRAPATE
                     TEETLARIYAEVLGLERIGVDDSFFDVGGDSLKAMQLVAAVSTTLDVELGVQTLFDAP
                     SVAALSQRLDKHERGSGRQDPADENEGEGLRPTIASVHGRGAAEAHASDLTLDKFLDE
                     ATLQAAPALPRPSAEASTVLLTGATGFLGRYLALEWLRRMDQVDGKLICLVRGKSNED
                     AWRRLEETFNSGDPDLLRHFRQLAADHLEVIAGDKGRADLGLDADTWQRLADTVDLIV
                     DPAALVNGILPYRELFGPNVVGTAELIRLALTTKLKPFAYVSTANVGDHVEPSAFTED
                     ADIRVISPSRKIDNSYTNGYANSKWAGEVLLREAHDRCGLAISVFRCDMILADTSYAG
                     QLNVSDLVTRMLFSLVATGIAPRSFYELDAAGNRQRAHFDALPVEFVAEAVATLGARA
                     IGGYQTYHVMNPHDDGIGVDEYVDWLIEAGTPIQRVDDFGEWLHQFKAALLALPDRQR
                     EQSVLQVLQLRFDDLHAAEPVHGSLAPTDRFRAAVQEAKVGPDNDIPHVSATVITKYV
                     SDLKLRGLL"
     gene            complement(9877..>20586)
                     /locus_tag="VGP27_22395"
     CDS             complement(9877..>20586)
                     /locus_tag="VGP27_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV7854080.1"
                     /translation="LPVTVNGKLDKRALPAPEYQDGDGYSAPTTLTEEILAGLFAQVL
                     GVDRVGVDDSFFDLGGDSLSAMRLISAMNEALDAHVAVRTLFEAPTVARLVPRIDDLG
                     GGLEPLVAGERPAVVPLSFAQNRMWVLNQLQGPSAVYNMAVALQLRGALDLEVLEAAL
                     ADVVGRHESLRTLFPAVDGIPQQLVVPAQEAEFGWQVVDASGWSTSQIDRAIHDASSY
                     TFDLDTEVPLRTTLFRLDHDKHMLVGVVHHIAADGWSVTPLVADLAAAYQARQQGRAP
                     SWAPLPVQYVDYTLWQRAQLGDVDDSESRVGEQLAYWQDALSGLPDWLELPTDRPYPA
                     EADHRGASVAVEWPAELQRRVREVARKHNATTFMVVQAALAVLLSKLSASSDVAVGFS
                     IAGRRDPALDELVGFFVNTLVLRVETVGDPSVDELIEQVRQRSLAAFENQDVPFEVLV
                     DRLNPSRSLTHHPLVQVMLAWQNFAGQQSEDPAVGLALGNVEVGQVQLETRTARMDLA
                     FALGERWSEAGEPTGLCGTVEFRTDVFDAASIEALIERLQRVLVAMTADSAQPISSVD
                     VLTAQEHARLDECGNRDVLSHPARVSTSIPAVFAAQVARAPEAVAVTCDGQSMSYREL
                     DEASSRLAHLLSSKGAGPGQRVALLFNRSVKAIVAILGVLKTGAAYVPVDPAHPDARI
                     EFVLGDSAPIAVVTTAGLAERLNGHDLLVIDADDTRIHAQPSTALPAPAPEDIAYLIY
                     TSGTTGVPKGVAVAHRNVIELLESLDADLGLAGQVWTLAHSLAFDYSVWEIWGPLLGG
                     GRLVVVPESVARSPEEFHALLVSEQVSMLSQTPSAFYALQTVDAQRPESGGQLKLQNV
                     VFGGEALEPQRLRTWLDNHPRLPRMINMYGITETTVHASFREIVDSDVDRNLSPIGVP
                     LPHLAFFVLDKWLRPVPAGVVGELYVAGGGLAVGYVGRASLSSTRFVACPFGGPGMRM
                     YRTGDVVRWGSDGQLQYLGRTDEQVKIRGYRIELGEIQAALASLDGVEQAAVITREDR
                     PGDKRLVGYITGTADPAGLRAVLAERLPAYMVPAAVVVIDALPLTVNGKLDIRALPAP
                     EYQDVDHYRAPDNAVEEILAGIYAEVLGLERVGVDDSFFELGGDSILSMQVVARARLA
                     GLLCRPRDVFVEQTVARLASVAEAPDGAAGEMDEGVGPVLATPIMRWLRDGNGVVDQF
                     NQTMVLQAPAGATQADVVVLLQALLDRHAMLRLHADDDGAGGWALHVPEPGSVDVGKY
                     LQTVDAISDVELVGARSRLSPAAGVMLSAIWATETRQLAVLIHHLAVDGVSWRILLED
                     LNIAWAQRYSGNPVELPAGGTSFARWSKLLADHAQTPEVAGHAEAWRQVAAVPAVLPS
                     PQPELDTYSTAGQMSVELDSETTRLLLGEVPSAFHAGVQDMLLIAFGLAWTEFLGTAG
                     APIGIDVEGHGRAEELAADVDLSRTVGWFTAKYPVALSVDGLSWAQVMTGDAALGPAI
                     KAVKEQLRALPDGLTYGLLRYLNPDVELAGSNSSIVFNYLGRLGAAGDLSDDMWRMDQ
                     DGMGLTAEATAAPIPLPHTVELNAGILEGDSGPQLHATWTWATSALTYDELSRVGQLW
                     FDALAGICAHVRAGGGGLTPSDIAPARLTQQQIDELQSAGPVADILPLTPLQRGLLFH
                     ATDTHGDPDDVYAVQVELALAGRLDVDRLRGAVETLVKRHPHLTARFCEHFDEPFQVI
                     SAEPRMPWRYVEFDAGDDQDEQVQQVLAAERAAVCDLAAQSPFRAALIRTAEDRHRFV
                     LTNHHIVLDGWSTPILLQEIFAGYYGHRLPAATPYRRFVTWLAERDIDAAHEAWREAF
                     IDFDSPALVGPAGRTELGERRVESHRVAENITAALSELARSHHATVNTVLQSAWAQLL
                     TSLTGHDDVAFGTAVSGRPADVSGADSMVGLLINTVPVRATINPHATVSDLLSQLQGF
                     TNNTLEHQHLALSDIHRATGHDQLFDTLFVFENYPVDVGALSGADGLAVTDFVAREQN
                     HYPLSVQAVPGTELLLRVEYDTDVFSSADIEALIGRYTVLLAAMTADPRRRLSSVDVL
                     DAVEHSRLDEWGNRAILAQPETPVSIPAVFGAQVARTPEAVAMTVGKSSWTYRELDEG
                     SNRLAHLLAENGARPGESVALLLPRSAEAIVAILAVLKSGAAYLPIDPAVPSARIEFM
                     LEDAAPIAAITTTGLTGRLAGQNLQVFDVTDAAIDEQPSTPLPTPQPDDLAHIIYTSG
                     TTGKPKGVAVTHRNVTRLFDSMDVGVEMAPGQVWAQCASYAFDYTVWEIWGALFHGGR
                     LVVVPESVMASPDDFHALLVTERVTVLSQTPTAVRMLSTEGLDFTSLMVAAEACPAEV
                     VDRWAPGRVMLNGYGPTETTVYATISAPLKASAGVVPIGVPVPGAALFVLDGWLRPVP
                     VGVVGELYVAGRGVSAGYIRRAGLTASRFVACPFDVPGGRMYRTGDLVRWGADGQLHY
                     LGRGDDQVKIRGYRIELGDIEAALAALEEVEHAAVILREDRPGDKRLVGYITESVSGT
                     VDPAETRGQLAERLPGYMVPSAVVVLEAFPMTVNGKLDKRALPAPEYGDTGGYREPAD
                     AVEGILAGIFSQVLGVERIGVDDSFFELGGDSILSMQVVAAARAAGLRCRPRDIFVEQ
                     TVARLARVVEVADSDGPADEGVGPVTATPIIRWLHSLGGADEFNQTVVVQAPAKVTAD
                     DVVVVLQALMDRHAMLRLRAGDDGAAGGWSLTVPEPGSARAGECLHTVDALTDEALLA
                     ARSRLNPATGAMLSALWVASTSQLVLMIHHLAVDGVSWRILLGDLNIAWAQHHSGQPV
                     ALPKAGTSFGRWASLLAEHAKSAQVVGQADAWRQVLATPSALPAAQPDMDTLATAGRL
                     SMSLDVQTTRMLLGEVPAAFHAGVQDVLLIAFGLALTAFLGGSTTPIGIDVEGHGRAE
                     EIADDVDLSRTVGWFTTKYPVALRVGGLSWAQVVAGEAALGAIVKDAKEQLRALPDGL
                     TYGLLRYLNADVDFGVPDPTIGFNYLGRLGGAAVDASEELWQVSVDGMSSIDAAAAVP
                     MPLAHSIELNAITMETDAGPALHANWSWARSALDHAQVSRLGQMWIDALAGICAHVRS
                     GGGGLTPSDIAPARLSQQEIDELQTQYEIADILPLTPLQQGLLFHSSAMRDSGSLGEL
                     YAVQVEFAITGPLKGRRLRDAVQAVLNRHPNLAARFIQQFDEPVQIIPADPAPVWRYI
                     DLEDLQAANLQPDEQVQRICADERAAVGAFTDDPAIRFVLIRTADERHRCVLTFHHIV
                     LDGWSMPILLREIFASYLGERLPAPGSYRDFVTWLADRDVDAARAAWRKLFAGFDTPT
                     LVGPPDRSALGQRGVERFSVPEETTRALDALARSHHTTINTVLQGAWALLLTSLTGQH
                     DVAFGVIVSGRPDEVAGADSMVGLAINTVPVRATLTAETTAADLLHHLQAAHNDTIEH
                     QHLALREIHRATGHDELFDSLIVYQNYPIDAGAQLSVDDLAITDFTSRESNHYPLTVQ
                     VHPGRELDVRVEYDTAVFSAARIDALMRRFTRVLVAMTADQESQS"
BASE COUNT         4037 a         6991 c         6106 g         3452 t
ORIGIN      
        1 tttgccaata gcaaattggc tttcgcgaga cggtcagcgt gttttgcccg gcagccagct
       61 tctataggtg ccgaagaagt ggcagatttc ctctgtgcga aaacggcgtt gccgaatagg
      121 tcagtaccga tacaaaccta cggctgcgtc agcaatctat gggagacgac caggtgtctc
      181 aacaaagcca tcacccatcg acagctatca cgatcagtac ggcaattgtt cttaaaagtg
      241 tggcctcaac cgtcgaggcg gcggcatcgt ctgaccaacg cgcacgttga cagcggccca
      301 cgcatcgggt tcccacgagt ggctacagca gtccacgaag tttcaggtcg ctgacgtact
      361 ttgtgataac ggtcgccgac acgtgcggga tatcgttgtc ggggccgacc ttcgcttctt
      421 gcaccgcagc acggaatcgg tcggtaggcg ccaaggatcc atgtacgggc tcggctgcgt
      481 gtaaatcgtc gaaacgcaac tgcaacacct gcaggaccga ttgctcacgc tgtcggtccg
      541 gcagggcaag cagcgccgct ttgaactgat gcaaccactc cccgaagtca tcgacgcgct
      601 gtatcggagt gccggcctcg atcagccagt ccacgtactc gtcgactccg atcccgtcat
      661 cgtgcgggtt catcacgtgg taggtctgat acccgccgat cgcacgagca ccaagagtgg
      721 caacggcttc ggcaacgaat tcgactggta gcgcgtcgaa gtgcgcgcgt tgacggttgc
      781 cggcagcgtc cagctcataa aacgagcggg gcgcgatgcc ggtggccacg aggctgaaca
      841 gcatccgggt gaccaagtct gaaacattga gctggcccgc gtaggaggta tcggccagga
      901 tcatgtcaca acggaacact gaaatcgcaa ggccgcaccg gtcatgagcc tcgcgcaaca
      961 gcacttcgcc agcccactta ctgttggcgt acccgttcgt gtagctgttg tcgatcttgc
     1021 gactggggct gatgactcgg atatcggcat cttctgtgaa cgccgacggt tcgacgtggt
     1081 cgcccacatt ggcagtagac acgtaagcga acggcttcaa tttcgtcgtg agcgcgagcc
     1141 ggatcagctc ggcggttcct acgacgttgg gtccgaacag ttcccgataa ggcaggatgc
     1201 cattgactaa cgccgcggga tcgacgatca gatcgacggt gtcggctagc cgctgccagg
     1261 tgtccgcgtc caagcccaga tccgcgcgac ctttgtcgcc cgctatgact tccaaatgat
     1321 ctgccgctag ttgccggaag tgccgcagta ggtcggggtc accgctgttg aaggtctctt
     1381 cgagacggcg ccaagcatcc tcgttggact tgccccgcac gaggcagatt agtttgccgt
     1441 ctacctgatc catccgccgc agccattcca aagccagata acgcccgagg aagcccgtcg
     1501 cgccggtgag cagaaccgtg ctcgcttcag cgctggggcg cggcagcgcg ggggcggctt
     1561 gcagcgtcgc ttcgtcgagg aatttgtcca gcgtcaggtc gctggcgtgc gcttccgcgg
     1621 cgccacggcc atggacggat gcaatggtgg gccgcagacc ctcaccctcg ttctcatcgg
     1681 cgggatcctg cctgcccgac cctcgctcgt gtttatccaa acgttgactc aaggccgcaa
     1741 cagatggagc gtcgaataac gtctggactc caagttcgac atcaagagtc gtgctgactg
     1801 ccgcgacgag ctgcatcgcc ttcaaagaat ctccgccgac atcgaagaag gagtcgtcca
     1861 cgccgattcg ttccagcccg agcacctcgg cgtaaatccg ggcgagcgtc tcctcagtct
     1921 ccgtggcggg ggcgcgatac cggtcggcat catcgtattt cggtgccggc agggtgtggg
     1981 tgtcaagttt gccgttgacc gtcaacggca gcgtctccaa cgccatcact gccgccggca
     2041 ccatataacg cggcaaccgc tcagccagca cgctgcgcgt ccaggccggg tctgccgttc
     2101 cggtgacgta ggcgaccagc cgcttatcac ccggccgatc ttcgcgagcg attaccgccg
     2161 cctgttccac gccatccaag ccgactagtg ccgcttgaat ctcaccgagc tcgatgcgat
     2221 acccgcggat cttgacctgc tcgtcggcgc ggcccatgta ctgcagctgc ccatcggcac
     2281 cccagcgaac caggtccccg gtgcgataca tccgctggcc cgcattgccg aacggacaag
     2341 ccacaaaccg tgcggccgtc agtccctccc ggcgcacata cccacatccg acgcctgcac
     2401 ccgcgacata cagctcgcca accacacccg tcggcactgg gcgcaaccat ttgtccaaaa
     2461 caaagagtgc ggccccgggc accggcgctc cgatcggcac cacccttgat cccgccttca
     2521 gcggggcgct catcgcgcta aacaccgtcg cctcggtcgg gccgtaggcg ttgatcattg
     2581 tccgaccgtg ggcccatcga tccatcagat cggtcgggca ggcttcgccg gccaccacta
     2641 gtgccatcgt gtccaagccc tcgggcgaaa gcatccccac cgcggatggg gtctgactca
     2701 agacatcaac ctgttcggcg accagcaggg cgtggaggtc gtccggtgat gccgcggccg
     2761 cttcggacac caccacgagt cgccccccag cgagcaatgg agcaaagatg tcccaaacag
     2821 agacgtcaaa agcgtaggag tgccactgtg accacacttt cgcgcgaggc agatccacgg
     2881 gcagcgtctc cagtaattga gtggcgttgc tatgtgagat agcaacacct ttggggacgc
     2941 cagtcgtacc ggaggtgtag atgatatagg cgatgtcgtc gggcgcgggc gccggcaagg
     3001 cggtgctggg ttgcacgtca atgcggagat cgttcactcg gatgatctga attccgaatt
     3061 cgacgagtcg ctcggctagt tccgctgtgg tgaccgcggc gacaggagcg gagtcgccga
     3121 gcatgaaccc tattcgtgcg tcggggtgcg ccggatccat cggcagatac gccgccccgg
     3181 ttttgagcac cgccaagatc gctacgaccg cctcagcgga gcgaggaaca agcagcgcga
     3241 cacactcacc cggaccggcc ccgtgctcac ccagcaggtg cgccagccga ttcgacgcct
     3301 cgtcgagttc ccggtaggtc atcgagcggc cttcacaggt gatcgccacc gcgtttggag
     3361 tgcgtcccag ttgggcctcg aacaacaccg gaatcgagtc agccgccggt gtcgactccg
     3421 tcaacatggc tcggttgccc cactcatgga gccgaaccaa ctcatcggcg tcgaccacgt
     3481 caaccgacga caaccgccga cccggatcag cggtcatcgc ggccaacaca cgcgcaagcc
     3541 gctcgatcag cgtctcgatg ctgcctgcgt cgaacacatc ggtgcggaac tccaccgccc
     3601 cgccaatccc gcccggctca ccgtcggctc cccagagttc ggccaacgaa aacgtcaaat
     3661 ccactcgtgc ggtgtcggtg tcggccgaca gaggggacac tcgcacatca cccaacaccg
     3721 aatcgaagtc ctgtccggcg aagttctgcc aggccaacat cacctgcacc agtggatgac
     3781 gattcaggct tcgggtcggg ttcagccgat cgaccaacac ttcgaagggc acatcctgat
     3841 gctcataggc ggccagactg cgctgacgca cctgcgccag cacgtcagcg attgtcggat
     3901 ccccatccag atccacccgc aacaccagcg tgttgacgaa gaacccgacc aactcatcga
     3961 gcgctggatc gctgcgcccg gcgattggga accccaccgc cacatcagaa ctggcgctga
     4021 ttttagccaa caggaccgcc agagcggcct gcatcaccat gaaactggtc gcgttgtgcg
     4081 cacgggccac ctcgcgcacc cgctgctgca actccaccgg ccaatccacc gccacacttg
     4141 aaccgcggta atcggctacc ggcggatacg gccgatcggt cggcaactgc agctgctcag
     4201 gcatcccggc cagcgcatcc atccagtagg ccaactgcgc ggcgatacgg ctattgctgt
     4261 cgtcaagctc gcccagctgc tcccgctgcc acaacgtgaa gtcgacgtac tgcaccgcca
     4321 acggcgccca gcccggggaa cggccctgcc gacgcgcctc ataagccaca ccgaggtcgc
     4381 cgaccaacgg gacgatcgac aagccgtcag cagcgatgtg gtgcactacc gccaccaaga
     4441 cgtgctcatc tgaagccacc cggaaaagtt gtgcccgcag cgggatctcg gctgtcagat
     4501 caaaggtgtg acgaaccgcc gcaccaatgg cctcctcgag ctggtctgcc gcccagccgg
     4561 tggcatcaac aacgtgccag ccgaagtcga cgctatcggc aggcatcacc agctgttggg
     4621 gtatcccttc atgggccacg aaccgggtcc gcaggctttc atgacggccc accacatcac
     4681 tgagcgcctg tcccaacgcc tgggcatcaa gcggcccgtt cagccgcaac gccactggca
     4741 tgttgtacac cggcgacggt ccgtgcaact tatccaagaa ccacaaccgg ttctgggcga
     4801 acgacaacgg caccgtagcc ggccgctcaa caggcaccaa gggctcaaaa ccagccgact
     4861 ccccacccac acgagaggcg agccgggcaa cggtttggga ctcgaacatc gaacggaccg
     4921 aaagacccgc atccaacgac ttgttcgccg cggcgatcac gcgcattgcg gagatactgt
     4981 ccccgcccag atcgaagaac gagtcgtcaa ccccgactcg ttgcacaccg agaacctgag
     5041 catagatgtc ggccagaagc tcttccaccg catttgacgg ggcgcgatac cgatcgaagt
     5101 cctggtactc cggtgcaggt aaggcgcgtt cgtcgatttt gccgttgacc gtcaatggca
     5161 gatcgttgag caccaataca gcagcaggca ccatataatt tggcagccgc tcagccaata
     5221 cggcacgcat ccccgcggga tcagcggcac cggtgacgta ggcaaccaga cgcttgtcgc
     5281 cgggatggtc ttcacggacg attaccgccg cctgatcgac tccgtccaga tccgtgagcg
     5341 ccgctacgat ttcgcccaac tcgatgcgat agccacggat cttgacctgc ttatccgcgc
     5401 ggcccaaata ctgcagttgg ccatcggcat cccagcgcac cacatcgccg gtgcgataca
     5461 tccgcgcacc cggagcaccg aacgggcatg ccacgaaccg cgtggacgac aacgacggcc
     5521 gccccacata tccgatcgcc aggccaccgc cggcgacgta caactcgccg atcacacccc
     5581 tcggcaccgg ccgcaaccac ttgtccagca cgaagaagcc gagatgggcc aacggcacgc
     5641 cgatcgggct gacgttgcgg tcggtgtcgc cgtcgacgat ctccctgaac gaggcatgca
     5701 ccgtcgtttc ggtgatgccg tacatgttga tcatccgcgg cgatctcgga tggttgtcca
     5761 gccacatccg aagtcgttgc ggttcaagcg cttcgccacc gaaaactacc gtctgaagct
     5821 cgagctgttg ccctcgctcg gagtgcagcg catcggcggt ttgcaacgca tagaacgccg
     5881 aaggggtccg gctcaacatg ccgacccgtt cactgaccat caaggcgtgg aagtcctctg
     5941 gtgagcgggc cgccgactcg gagatcacga cgacacggcc gccacgcagt agcgcaccga
     6001 agatttccca gaccgagaag tcgaacgcca acgagtgaga ctgtgtccag acctgcccgg
     6061 ctaattccaa gtcgccgtcc agagagtcca gcaactgagt cacgttgcgg tgtgggatcg
     6121 caacaccttt ggggacaccg gtggtgccgg acgtgtagat gatgtaagcg atatcgtctg
     6181 gtgccggcgc cggcagtgcc gtgcacggct gcgcggccac tgcagcatcg ttgacaccga
     6241 tgatggccgc attcgacccg tgcaaccgca ttgccagctc tgcggtagtg gtgaccgccg
     6301 cgatcggggc ggcgtcttcg agcatgaagc cgatgcgagc gtcgggatgc gctggatcca
     6361 tcggaaggta ggccgccccc gtcttgagca ctgccattat ggctacgacc gcctcagcgg
     6421 aacgcggaat cagcagtgcc acacacctgc cgggcctcgc tccttgtccg atcagcaagt
     6481 gggccaaccg gttcgatgcc tcatcgagtt cgcgataagt catcgagcga tcatcgaaag
     6541 tcaccgctac tgcctgcggg gtacgcgccg cctgtgcagc gaacaacacc ggaatcgata
     6601 ctcctgtcgg cgcaggcttg gccagcaccg cccggttact ccacccatcg agccgggcca
     6661 actcaccggc atcgagcacg tccaccgacg acaactgccg gcccggatca accgtcatcg
     6721 ccgccaacac ccgcgccaac cgctcaatca gcgtctcgat gctggctgcg tcgaacacat
     6781 cggtgcgata ctccaccagc ccgccgatcc cggccggctc accgtcatcg gtccagcgct
     6841 cggccaacga aaacgtcaga tctacccgcg cggtgtgggt ttcggctgac agcgggctgg
     6901 ccttcacatc gcccagtgcc aatccaccgg caccctcgtt gacagcgccg gcgaggttct
     6961 gccagcccaa caacacctgc accagcggat ggtgagccat cgagcgggcc gggttgagcc
     7021 ggtccaccag cacctcaaac ggcacatcct gatgctcgaa cgctgccagg ctgcgctggc
     7081 gcacctgggc cagcaactct gacgtggtgg gatttccgct cagatcaatc cgtaacacca
     7141 tggtgttgac gaagaacccc accaagtcgt caagggctgc gtcgctccgt ccggcgattg
     7201 ggaatcccac cgccacatcg gaattggcgc tcagccgaga taggaggacc gccagcgccg
     7261 cctgcaccac catgaagctg gtcgcattgt gcgtgcgagc cgttgcatgt atctgctgct
     7321 gcaacgccgc cggccactcc accgccaagt gcgcgccacg ataatccgct accgtcggat
     7381 acggacggtc cgttggcaac tccaaccgct ccggcaaacc ggccaacgcg tcctgccaat
     7441 aggccacctg tccagcaatg cggctatcgc tgtcttccag ttcccccaag tgcccacgct
     7501 gccacaacgt gtaatccacg tactgcaccg gcaacggggc ccactccggc gccttccctt
     7561 gttgccgggc cagatacgcc gcacccaggt ccctcaccag tggtgacgcc gacaaaccgt
     7621 cagcagcgat gtggtgcacc accgccacga gcacgtgctc cgtgtcgttg acatggaaaa
     7681 gccgtgcttg cagcggaatc tcggctgcca ggtcaaatgc gtagcccgcg atctcttcga
     7741 cggcctcgct cagttgattc gccgaccacg cggtggcatc gacgatgtcc caaccgaagt
     7801 caatgtgatc cgccgacaga accgattgct gcggagtccc gtcaaccaca gggaacagcg
     7861 tgcgcaaggt ttcatggcgg ctcaccacgt cggccagcgc cacttgtagc gcctcggtgt
     7921 tgaggagccc gctcagccgc aacgccacgg gcatgttgta aatcggtgaa ggcccgtgca
     7981 attgatctaa gaaccacaac cggttctgag cgaaagacag cggcaccacc gctggccgct
     8041 cgacagccac caatggcaca gacacatcac cgtctacacc gactcgagac gccagctcgg
     8101 cgaccgaagg tgcctcaaac agcacccgca ccgacagtcc ggcatctaga gacctgttga
     8161 ccgccgcgat cgcccgcatc gccagaatgc tgtccccgcc gaggtcgaag aatgagtcat
     8221 cagctccgac ccgcttgact ccgagcacct ggctgtatat ttcggccaga atctcctcgg
     8281 tggggttggc cggcgcacga tagcgtttag ccgtctcctg gaattccggt gccggcaggg
     8341 ctcgaacgtc gagtttgccg ttaatcgtca gaggcaacgc ctccaggacc acaaccgccg
     8401 cgggcaccat gtacgccggc aaccgctcgg ccagaaccga gcgcgccctg atcgggtcgg
     8461 ggtctccggt gacatagcca accagccgct tgtcaccggg gcggtcctcg cgggcgatga
     8521 ccgccgcttg ctccacacct tcgaacgcgg ccaacgcgga ctgcacctca ccgagctcga
     8581 tccggtaacc gcggatcttg acctgctcgt caacgcgccc taagtactgc agctgcccgt
     8641 cagcactcca gcgcaccagg tcaccggtgc gatacatccg cagcccagga cgtccgaacg
     8701 ggcacgccac gaacctcgac ccggtcaacc cggcccggcc cacatatccg acgcccacgc
     8761 cgttgccggc gacatacaac tcaccgacca caccgaccgg cattgtccgc aaccacttgt
     8821 ccaacacaaa caatgccgcc cctggcaccg gcgagccgat cggaggcgca ccggtgcccg
     8881 gttcaagtgg cgcactgatc gccacacaca tcgtcgtttc cgttgggccg taggcgttga
     8941 tcatgacccg accgggggcc cacctatcca ccacctcgga agggcaggcc tcaccggcca
     9001 tcaccaacgc gagcgattcc aacccttctg acgacagcac gcccatggca gacggggtat
     9061 tggtaagcac cgtgacgcgc tctgcgacca gcaggtcgtg gaagtcgtcg ggtgagctca
     9121 tcaccgcttc ggtgaccacc accactcgcc cgccgctaac caagggggcg aggatctccc
     9181 acgccgagac atcgaaggac agggaatgac aaagcgccca cacgccgggg cgcggcaggc
     9241 cggcgtccag tgactccaat aactgggcca cgttgcgatg gcttatcgca actcctttgg
     9301 ccacaccagt cgtccccgag gtgtagatga ggtaggcgat gtcttccggt cccggtgccg
     9361 tcatcccggt actcggacgg gtttcagcgg gggcgtcctc gactgcgacg accgcgacgt
     9421 tccggccgtc caagcgcgac cgcaacgcct cggtggtgag cgcggcgacc ggccccgagt
     9481 cctcgagcac gaaatcgata cgcacggccg ggtgcgctgg gtcgatcggc agatacgccg
     9541 ccccggcctt cagcacagcc agcaccgcca caaccgcctc gatggagcgg ttgagtagaa
     9601 ccgcgacgca ctgaccgggt cccgctccct cggcagccaa ccgatgcgcc aaccgatcag
     9661 cggcttcgtc cagctgccga taggtcatcg agtggccgtc gaaggacacc gccaccgcct
     9721 ccggggagcg agtcacgtgc tgggcgaaca actccgggat cgacgccgat gcgcccaccg
     9781 gcatatcaag caccgcccga ttggcccact catcaagctg ctcgtgctcg tcgtcgtcga
     9841 acaggtcgat cgacagcaac cgcgttgcgt cggcggtcat gattggctct cctgatcggc
     9901 ggtcatggca actaataccc gcgtgaaccg tcgcatcagc gcgtcgatgc gcgccgcact
     9961 gaacacggcg gtgtcgtact cgacgcgcac atcgagttcg cgtcctggat ggacttgcac
    10021 tgtcaggggg tagtgattgg attcacggct ggtgaaatcg gtgatggcca ggtcatcgac
    10081 gctcagctgt gcacctgcgt cgatcgggta gttctggtag acgatgaggc tgtcgaacaa
    10141 ctcgtcatga cctgtggcgc ggtggatttc gcgtagcgcc aagtgctggt gctcgattgt
    10201 gtcattgtgt gccgcctgca ggtggtgcaa cagatcggcg gccgtggttt cggcggtaag
    10261 ggtcgcccga accggcacgg tgttgatcgc cagaccgacc atcgagtcgg cacccgccac
    10321 ctcgtccggt cgtcctgaca cgatgactcc gaacgcgacg tcgtgctggc cggtgagcga
    10381 ggtcagcaac agcgcccatg cgccttgaag gaccgtgttg atagtggtgt ggtgcgagcg
    10441 cgcaagtgcg tcaagtgctc gagtggtctc ctcaggaacc gaaaaccgtt ctacaccacg
    10501 ctgacccagc gctgatcgat ccggcggccc aaccaaggtg ggggtgtcga agccagcgaa
    10561 aagctttcgc cacgcggcgc gcgcagcatc gacgtctcgg tcggccagcc aggtcacaaa
    10621 gtcgcgatag gaaccaggcg ccggaagccg ctcccctagg tagctcgcga agatttcgcg
    10681 cagaaggatc ggcatcgacc aaccatcgag aacgatgtgg tggaaagtga ggacacagcg
    10741 gtgccgctcg tcagcagtgc gtattagaac gaaccggatg gcagggtcgt cggtgaaagc
    10801 gccgacggcg gctcgctcat cggcacagat ccgctgaacc tgctcatcgg gttgcagatt
    10861 cgccgcctgg agatcctcga ggtcgatgta tcgccatacc ggcgccggat cggcgggaat
    10921 gatctgcacc ggctcatcga actgctggat gaagcgggcg gcgaggttcg gatggcggtt
    10981 cagtacagcc tgtaccgcat cacgtagcct gcggcctttc aaaggtccag tgatggcgaa
    11041 ctcgacctgc actgcataga gctcgcccaa gctgccagag tctcgcatgg cgctggaatg
    11101 gaacagcaga ccttgctgca gcggagtgag cggcaggatg tcggcgatct cgtattgcgt
    11161 ttggagctcg tcgatctcct gctggctgag gcgcgccggg gcgatgtcag acggcgtcag
    11221 ccctccgccg ccgctgcgta cgtgcgcgca aataccggcc agggcgtcga tccacatctg
    11281 gcccagccgg ctgacctgag catgatccag cgccgaacgc gcccacgacc agttggcgtg
    11341 cagcgcggga ccggcatcgg tttccatagt gattgcattg agttcgatgc tgtgcgccaa
    11401 cggcattggc actgccgccg cggcatcaat cgacgacatt ccgtcaacac tgacctgcca
    11461 tagctcttcg gatgcatcca ccgccgcccc gcccagacgc ccaagatagt tgaagccaat
    11521 cgtgggatcc ggaacgccga agtcgacatc ggcgttgagg tagcgcagca ggccataggt
    11581 cagcccgtca ggcagcgcac gcaactgttc cttcgcgtcc ttgacgatcg cgcccagggc
    11641 cgcctcaccg gccaccacct gtgcccagga cagcccgcca actctcaacg ccaccgggta
    11701 cttcgtcgtg aaccatccga ccgtgcggga caagtcaacg tcgtcggcta tctcctcggc
    11761 gcgcccgtgt ccctcgacgt cgataccgat cggtgtcgtt gagccgccga ggaatgcggt
    11821 cagtgctaag ccgaacgcga tcagcaacac gtcttgcacc ccggcatgga acgctgcggg
    11881 cacctcgccc agcagcattc gagtcgtctg cacgtcgagc gacatcgaca ggcgaccggc
    11941 ggtggccagg gtatccatat cgggctgtgc cgcaggcagc gccgacgggg tcgccaagac
    12001 ctgccgccac gcgtcggcct ggcccactac ctgcgctgat ttggcatgct cggccagcag
    12061 cgatgcccac cggccaaacg aggtgcctgc cttgggtaac gcgaccggct gcccgctgtg
    12121 gtgctgcgcc cacgcgatat tgaggtctcc caacaggatt cgccacgata cgccgtcgac
    12181 ggccaagtgg tggatcatca gcaccaactg gctcgtcgaa gcgacccaca gcgcactcag
    12241 catcgccccg gtagccgggt tgagtcgcga tctggccgct aacagcgcct cgtccgtcag
    12301 cgcatcaacg gtatgaagac actcgccagc tcgagccgac cccggctcgg gcacggtcaa
    12361 cgaccagccc ccagcggcgc cgtcgtcgcc ggcacgcagt cgcagcatgg catgccggtc
    12421 cattaacgcc tgcaacacca ccacgacgtc atctgcagtc accttggccg gcgcttgcac
    12481 caccacggtc tggttgaact catccgctcc gccgaggcta tgcagccagc ggatgatcgg
    12541 tgtcgctgtc accggaccga cgccctcgtc ggccggaccg tcggaatcgg cgacttcgac
    12601 cacacgggcc agtcgggcga cagtctgctc gacgaaaatg tcgcgcggac ggcaccgcag
    12661 accagcagcc cgcgccgcag ccaccacttg catcgacaag atgctgtcgc cgcccagttc
    12721 gaagaacgag tcgtcgacgc cgatccgctc cacacccaac acctggctga atatgcccgc
    12781 caggattccc tcgaccgcat cggcaggctc ccgatagccg ccggtgtcgc cgtactccgg
    12841 cgctggcagc gcacgcttgt cgagcttgcc gttgaccgtc atcggaaacg cctcgagcac
    12901 caccaccgcg ctcggcacca tatagccggg cagccgctcg gccaactgcc cacgcgtttc
    12961 ggccgggtcg actgtgccac ttaccgattc ggtgatgtag ccgaccagtc gtttatcacc
    13021 gggccgatcc tcgcggagga tgacagcagc gtgctcaact tcctccaagg cagcgagcgc
    13081 cgcctcgatg tcgcccaact cgatgcgata accgcggatc ttgacctggt cgtcaccgcg
    13141 ccccaggtaa tggagctgtc catcggcacc ccagcgcacc agatcgccgg tgcgatacat
    13201 ccgcccgccg gggacatcga acgggcacgc cacgaaacgc gatgcagtca acccagcccg
    13261 acgaatatat ccggcgctca cgccgcgtcc ggccacatac aactcaccga ccacacctac
    13321 cggtaccggc cgcaaccacc cgtcgaggac gaacaatgcc gccccgggca ccggcacgcc
    13381 gatcggcacc acaccggcgg acgccttgag cggcgcactg atggtggcgt acacagtcgt
    13441 ttcggttggg ccgtagccgt tgagcatcac tcggccggga gcccatcgat cgaccacctc
    13501 tgccgggcag gcttcggccg caaccatcaa tgatgtgaag tccagaccct cggtcgaaag
    13561 cattcgaacc gctgtggggg tttggctcaa tacagtgacc cgttctgtca ccagcaaggc
    13621 gtggaagtcg tcgggtgacg ccatcacgga ctcggggacc accacaagcc gcccaccatg
    13681 gaacagcgcg ccccagatct cccacactgt gtagtcgaaa gcgtaggacg cgcattgcgc
    13741 ccaaacttgt ccgggcgcca tttcgacacc gacatccatc gaatcgaaga ggcgggtcac
    13801 gttgcgatgg gtcaccgcaa cgcctttcgg tttacccgtc gttcctgagg tgtagatgat
    13861 gtgagcgaga tcgtcaggct gcggcgtcgg cagtggcgta ctgggctgct cgtcgatggc
    13921 cgcgtcagtg acatcaaaaa cctgtaggtt ctgcccggcc agtcggccgg tcaacccagt
    13981 tgtcgtgatc gcggcgatcg gggcggcgtc ttcgagcatg aactcaatac gcgccgacgg
    14041 cacagccgga tcgatcggca agtaagcggc accgctcttg agcacagcca gaatcgccac
    14101 gatcgcctca gctgaccggg gaagcagcag cgcgacgctt tcgccaggtc gcgcaccatt
    14161 ctccgccagc agatgcgcta gccgattcga gccctcatcg agctcacgat aggtccacga
    14221 agactttcca acagtcatcg cgacagcctc gggggtccgg gctacctgtg cgccgaaaac
    14281 cgcagggatc gataccggtg tctcgggttg ggccagtatg gcgcggttgc cccactcgtc
    14341 gagtcgcgag tgttccacag cgtcaagcac atccaccgac gacagccgcc gccgcgggtc
    14401 tgcggtcatc gccgccagaa gcacggtgta gcggccgatc agcgcctcga tgtccgctga
    14461 agagaagaca tcggtgtcgt actcgactcg caggaggagc tcggttcccg ggactgcttg
    14521 gacgctcagc gggtagtgat tctgttcgcg ggcgacgaag tctgtaacgg ccaacccgtc
    14581 cgcgcccgac agcgcgccga cgtcgacggg gtagttctcg aatacgaaga gggtgtcgaa
    14641 caactggtcg tggccggtgg cgcgatggat atcactcagc gccaagtgct ggtgctcgag
    14701 cgtgttgttg gtgaagccct gcagttggct caacaggtcg gagacggtgg catgcggatt
    14761 gatggtggct ctgaccggca ccgtgttgat caacaagccg accatcgagt cggctcctga
    14821 tacatcggct ggccggcccg acaccgcggt gccgaaggcg acatcatcgt gacccgttag
    14881 tgaggtgagc aattgcgccc aggcgctctg cagcacggtg ttgacggtgg cgtgatgcga
    14941 ccgcgcgagc tcactgagcg cggcggtgat gttctcggcc acgcgatgtg attcgacgcg
    15001 tcgctcgccc aactccgttc ggcccgcggg gccgaccagt gcgggactgt cgaagtcgat
    15061 aaacgcctct cgccaagcct cgtgagcagc gtcgatgtcg cgttcggcga gccatgtcac
    15121 gaacctgcgg tagggcgtgg ccgcaggcaa ccgatgcccg taatagccgg cgaatatttc
    15181 ctgcagcaat atcggcgtcg accaaccgtc gagcacgatg tggtggttcg tcagcacaaa
    15241 ccgatgccgg tcttcagcgg tgcggatcaa cgcagctcga aacggcgatt gagcggcgag
    15301 gtcacagacc gcggcacgtt cggcggcgag cacttgttga acttgctcgt cctgatcgtc
    15361 gccggcgtcg aattcgacat agcgccatgg catccgcggt tcggcagaaa tcacctggaa
    15421 cggctcgtcg aaatgctcgc agaaacgagc cgtcaagtga ggatgacgct tgaccagcgt
    15481 ctctactgca ccgcgaagcc ggtcgacgtc gagtcggccg gccagggcga gttccacctg
    15541 gaccgcatac acgtcgtcgg ggtcgccatg cgtgtccgtg gcgtgaaaaa ggagtccccg
    15601 ctgcaagggt gtgaggggca ggatgtcggc gactggcccc gcagactgca gctcgtcgat
    15661 ctgctgctgg gtgagccggg ccggagcgat atcggatggg gtcaacccgc ccccaccggc
    15721 tcgcacatgt gcacaaatgc cggccagtgc gtcgaaccac agctggccga cacggctcag
    15781 ctcgtcatag gtcagcgccg acgtcgccca cgtccacgtc gcgtgcagtt gtggaccgct
    15841 gtcaccttcc agaatgccgg cgttcagttc cacggtgtgc ggcagcggta ttggggccgc
    15901 tgtggcctcg gccgtaaggc ccataccgtc ttggtccatc cgccacatat cgtcggaaag
    15961 gtcgcccgcg gcgccgagac gtccgaggta gttgaagacg atcgacgaat tggagccggc
    16021 gagttcgaca tcgggattca ggtagcgcag caatccgtag gtcagcccgt ccggcagggc
    16081 gcgcagttgc tctttgacgg ccttgatggc tgggccgagc gcggcatcgc cggtcatcac
    16141 ctgcgcccag gagagcccgt cgacgctcag cgccacaggg tatttggcag tgaaccaacc
    16201 cacggtgcgt gacagatcca catcggcggc caattcttcg gcccggccat ggccttccac
    16261 atcaatgccg atcggcgcgc cggctgtgcc caagaattcc gtccacgcca acccgaaggc
    16321 gatcaacaac atgtcctgta ccccagcgtg gaaggccgaa ggcacctcac cgagcagcag
    16381 ccgggttgtt tcggagtcga gctctaccga catctgccct gcagtggaat aggtatccag
    16441 ttcaggttga ggcgaaggca ataccgctgg aaccgccgct acttgccgcc acgcttcggc
    16501 atgacccgcc acctcggggg tttgcgcatg gtcggccagc aacttcgacc accgggcgaa
    16561 cgacgtgccc cctgccggaa gctcgaccgg attcccgctg taacgctgcg cccacgcgat
    16621 gttcaagtct tccaacagta tccgccacga cactccgtca accgccagat ggtggatgag
    16681 gaccgctaat tggcgcgtct ctgtcgccca tatcgccgaa agcatcactc cggctgccgg
    16741 gctcaaccgc gaccgagcgc ctaccaactc gacatctgat atcgcatcga ctgtctgcag
    16801 gtattttcca acatcgaccg aaccgggttc gggcacgtgc agcgcccaac ccccggcgcc
    16861 gtcgtcgtcg gcatgcagcc gcagcatggc atgtcgatcc agcagggcct gcaacagaac
    16921 caccacgtcg gcttgggtcg ctcctgcggg ggcctgtagc accatcgtct ggttgaactg
    16981 atcaaccacg ccgttcccgt cacgaagcca gcgcataatc ggggtggcca atactggccc
    17041 gacaccctca tccatctcgc cggccgcgcc gtcgggcgcc tcggccacac tcgccagccg
    17101 ggccacagtc tgctccacga agacgtcccg cggccgacat aacaggccgg cgagccgcgc
    17161 ccgcgcgacc acctgcatcg acaggatgct gtcgccgccg agctcgaaga acgagtcatc
    17221 aaccccgacg cgttccaacc ccagaacctc ggcatagata ccggccaaga tttcttcgac
    17281 tgcattgtcg ggggcacggt aatgatcgac gtcttggtac tcaggtgccg gcagggctcg
    17341 gatgtcgagt ttgccgttga ccgtcagcgg cagcgcgtcg atgacgacca cggccgcggg
    17401 caccatatag gcaggcaacc gctcggcgag cactgcgcgc aatccggccg ggtctgccgt
    17461 tccggtgata taaccgacga ggcgtttgtc gccggggcgg tcttcgcggg tgatgaccgc
    17521 ggcctgctcc actccatcga ggcttgcaag cgccgcctgg atctcaccca attcgatgcg
    17581 atagccgcgg atcttgacct gttcatcggt tcggcccaga tactgcaact ggccatctga
    17641 tccccaacgc accacatccc cggtgcggta catccgcatt cctggcccac cgaacggaca
    17701 agcaacaaac cgcgtggagg acaacgatgc ccgacccacg tatccgactg cgaggccgcc
    17761 gcccgcgacg tacaactcac ctaccacacc cgccggcacc gggcgcaacc acttgtcaag
    17821 cacgaaaaag gccaaatggg gcagcgggac accgattggg ctcagattgc ggtcaacgtc
    17881 actgtcgacg atctcacgga atgaggcatg cacggtggtc tcggtgatgc catacatgtt
    17941 tatcatccgc ggtagtcgcg gatgattgtc gagccacgtc cgaaggcgtt ggggttcaag
    18001 ggcttcacca ccgaatacca cattctgaag cttcagctgc ccgccagact cgggccgttg
    18061 cgcatcgacg gtttgcaacg catagaacgc cgacggcgtc tgactcaaca tgctgacctg
    18121 ttcgctgacc agcagcgcgt ggaattcctc gggagagcgc gctaccgact cgggtaccac
    18181 caccagccgc ccgccaccga gcagcggtcc ccagatctcc cacaccgaat agtcgaacgc
    18241 caacgaatgg gccaatgtcc acacctggcc cgccagcccc aggtcggcgt ccaacgactc
    18301 caacaactcg attacgttgc ggtgggccac cgcaacgcct ttgggcacac ctgtcgtacc
    18361 cgaggtgtag atgaggtaag cgatatcctc cggcgcgggt gccggcaatg cggtactggg
    18421 ctgggcatgg atgcgcgtgt cgtcggcgtc gatgaccaac aaatcatggc cgttcagccg
    18481 ctcggccagc cctgcggtgg taacaaccgc gatcggagcg gagtcgccaa gtacaaattc
    18541 gatccgcgca tcagggtgcg ctggatcgac cggtacgtag gcggcaccgg tcttgagcac
    18601 tcccagtatc gccacgatcg ctttgacgga acggttgaac agcaacgcaa cccgctgtcc
    18661 cggtccggcg cccttactcg acagcaggtg tgccagacga ctggacgcct catcaagttc
    18721 gcgatagctc atcgactggc catcgcacgt caccgccacc gcctccgggg cacgggccac
    18781 ctgcgcggcg aatacagctg gaatcgaggt cgacaccctt gccgggtgac tcaatacgtc
    18841 cctgttaccg cactcgtcca ggcgcgcgtg ctcttgcgct gtgagtacat ccaccgacga
    18901 aatcggctgc gccgaatcgg cagtcatagc caccagcacc cgctgcagcc gttcgatcag
    18961 cgcctcgata ctggccgcgt cgaagacgtc ggtgcgaaac tccaccgtcc cgcaaagacc
    19021 cgtcggctca ccggcctcac tccagcgctc gccaagcgcg aacgccaggt ccatgcgagc
    19081 ggtacgagtc tccaactgca cctggccgac ctcaacgttc cccaatgcca aaccgacagc
    19141 gggatcttcg gattgctgcc cggcgaagtt ctgccaggcc agcatcactt gcaccagcgg
    19201 atggtgagtc aagcttcggc tcgggttcag ccgatcgacg agcacctcga acggcacatc
    19261 ctgattttca aacgcggcca agctgcgctg gcgcacctgc tcgatcaatt catcgaccga
    19321 cggatctccg actgtctcga ctcgaagcac taaagtgttg acgaagaatc ccacgagctc
    19381 atcgagggcc gggtcgcggc gtcccgcaat cgagaagcca accgctacat cggaactggc
    19441 gctgagcttc gacaacagca ccgcaagagc agcctggacc accatgaagg tcgtagcgtt
    19501 gtgcttgcgg gcgacctcgc gcacccgccg ctgcaactcg gctggccact ccaccgccac
    19561 gctggctcca cggtgatcgg cctccgccgg atacggtcga tccgtgggca actctaacca
    19621 gtcagggaga cccgacagcg catcttgcca gtaggcgagc tgctcaccga cacgactctc
    19681 gctgtcgtct acatccccca actgcgcacg ctgccacagc gtgtaatcga cgtactgcac
    19741 cggcagcggc gcccaactcg gagcccgacc ctgttgacgg gcttgatacg ccgcggccag
    19801 gtcggccacc agcggggtga ccgaccagcc gtcggcggcg atatggtgca ccacccccac
    19861 cagcatgtgc ttgtcatggt cgagccggaa cagcgttgtg cgcaagggaa cttcggtgtc
    19921 gagatcaaac gtgtagctgc tggcgtcgtg gattgctcga tcgatttgcg acgtcgacca
    19981 tccgctcgcg tcgacgacct gccaaccgaa ttcggcttcc tgggccggca ccaccagctg
    20041 ctgcgggatc ccgtcgaccg ccgggaacag cgtccgcaag ctctcgtggc ggccaacgac
    20101 atcggccagc gccgcctcta gcacctcaag atcgagtgcg ccgcgcagct gcagcgccac
    20161 cgccatgttg tataccgctg aggggccctg cagctgattc agaacccaca ttcggttctg
    20221 tgcgaacgac aacggcacca ccgctggccg ctcaccagcc accagcggct ccaacccgcc
    20281 acccaggtca tcgatccgag gcaccagtcg cgcaaccgtc ggtgcctcga acagagtgcg
    20341 caccgcaacg tgggcgtcga gggcttcgtt catcgcggaa atcagccgca tggcagacag
    20401 cgaatctccg cccaagtcga agaatgagtc atctactccg actcgatcca cgccgagcac
    20461 ctgagcgaag agtccggcaa ggatctcctc ggtcagggtg gtcggggcgc tgtacccatc
    20521 gccgtcctgg tactccggtg ccggcagggc gcgcttgtca agcttgccgt tcaccgtcac
    20581 cggcaa
//
LOCUS       DASYLX010000961         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3022379,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000961 DASYLX010000000
VERSION     DASYLX010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022379"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VGP27_22400"
     CDS             <1..1384
                     /locus_tag="VGP27_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEV7854081.1"
                     /translation="ARNHRTPAGAVKPTVVIIGAGSVEFTRELLGDILSFPELSSVRV
                     VLHDIDDERLETAEAIARATARAAGADPEVVATRDRRRALDGADYVINVIAVGVHEAT
                     VRDFEIPARYGLNQTIGDTIGVGGIFRGLRTFPVLADIARDMAEVCPDAWLLNYTNPM
                     AMNISFLHAVAPRLKVLGLCHSVYWTMVGLCELIDVPYDEVSYWSAGVNHQAWVLRWE
                     RRGENLYPLLDERIAADPELRRRVRVDMYRRLGYYPTETSEHSSEYVPWYLHHPDEIE
                     RLRINVGEYVSISEANVAEYHRVRTELADTQSLAIDTGSTEYAPQVIHSMETGTPRVI
                     SANVVNDGLITNLPDGVAVEVPTLLDALGAHPMKVGDLPPQCAALNRNFLGPVDLTVR
                     AAVDGDPRLVRAAAMVDPNTAATLTVDQIWHLCDDLTAAHGDLLPPALRSRSDVAAGA
                     APNLARDFAN"
     gene            1397..>2292
                     /locus_tag="VGP27_22405"
     CDS             1397..>2292
                     /locus_tag="VGP27_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7854082.1"
                     /translation="MIRRKLCIPLVAVLAFVVAACGGGGDSSSGPTEIAVWHGYQDTE
                     GDVFKGLIDQYNKDHPDVKVSELYSSNDLVLQKVLTAVRGNSAPDVAYMFGSWSPNIA
                     KIPQVVDMKDEVSKPDWKWDDFYQAERDATKVGDKVVGVPALVDNLAIVYNKKLFADA
                     GVAPPTPDWTWDDFRAAAAKLTDPAKGQFGWLIPADGSEDTVWHYIPMLWEAGGNILT
                     PDNTKAAFNSEAGVKALTTLQQMAVDDKSLYLDTTNENGPKLMNSGKIAMLVTGPWDL
                     SSLSDIDYGVQVMPTYAGSNGG"
BASE COUNT          413 a          796 c          725 g          358 t
ORIGIN      
        1 cgcccgcaac caccgaacac cggccggtgc agtgaaaccc accgtcgtca tcatcggcgc
       61 gggcagcgtc gagttcaccc gcgaactgct cggcgacatc ctgtccttcc ctgagctgag
      121 ttcggtccgg gtggtgctgc acgacatcga cgacgaacgg ctggagacgg ccgaggccat
      181 cgcccgcgcc accgcccgcg cggcgggtgc cgacccggag gttgtcgcca cccgcgatcg
      241 ccgccgggca ctcgacggcg cggactacgt catcaacgtc atcgcggtcg gcgtgcacga
      301 ggccacggtc cgcgacttcg agatcccggc gcgatacggg ctgaaccaga ccatcggcga
      361 cacgatcggc gtcggcggaa tcttccgcgg cctgcgcacc tttcccgtcc tggccgacat
      421 cgcccgcgac atggcggagg tgtgccccga cgcctggttg ctcaactaca ccaacccgat
      481 ggccatgaac atctcgttcc tgcacgcggt cgccccgcgg cttaaggtac tcggcctgtg
      541 tcactccgtg tactggacaa tggtcggcct gtgcgaactc atcgacgtgc cctacgacga
      601 ggtgtcctac tggtctgccg gtgtgaacca ccaggcgtgg gtgctgcggt gggagcgccg
      661 cggcgagaat ctctatccgc tgctggacga gcgcatcgcc gccgatccgg agctgcgcag
      721 gcgtgttcgc gtcgacatgt accggcggct gggctactac cccaccgaaa ccagcgagca
      781 cagcagcgaa tacgtgccct ggtatctgca tcatcccgac gagatcgagc ggctgcggat
      841 caacgtcggc gagtacgtgt ccatcagcga agccaacgtg gccgaatacc accgggtgcg
      901 aacggaattg gcggacacgc agtcgctggc gatcgacacc ggttccaccg agtacgcccc
      961 ccaggtgatc cactccatgg agaccgggac accgcgggtc atctcggcca acgtcgtcaa
     1021 cgacgggctg atcaccaatc tgcccgacgg cgtggcggtc gaggtgccca cactgctcga
     1081 cgcgttgggc gcgcacccga tgaaggtggg cgaccttccg ccgcagtgcg cggcgctcaa
     1141 caggaatttt ctcgggccgg tcgacctcac ggtgcgcgcg gccgtcgacg gcgacccgcg
     1201 tttggtgcgc gcggcggcca tggtcgatcc gaacaccgcc gcgacgctga ccgtcgatca
     1261 gatctggcac ctgtgcgacg acctgaccgc cgcgcacggc gacctgctac cgccagcgct
     1321 gcgctcccgg tccgatgtcg ccgccggcgc ggctccgaac ctcgcccgag atttcgccaa
     1381 ctaaggagtg tccgccgtga ttcgacgcaa gttgtgcatc cccctggtcg ccgtcctcgc
     1441 cttcgtcgtc gccgcgtgcg gtggcggcgg cgactcgtcg tccggcccca ccgagatcgc
     1501 ggtgtggcac gggtaccagg acaccgaggg cgacgtgttc aagggactga tcgaccagta
     1561 caacaaggac catccggacg tcaaggtgtc cgagttgtac tcctccaacg acctcgtgct
     1621 gcagaaggtt ctcaccgcgg tccggggcaa cagcgcaccg gacgtcgcct acatgttcgg
     1681 gtcctggtcg ccgaacatcg ccaagatccc gcaggtcgtc gacatgaaag acgaagtgtc
     1741 gaaacccgac tggaaatggg acgacttcta tcaggccgag cgggatgcca caaaggtcgg
     1801 cgacaaggtc gtcggcgtcc ccgcactggt ggacaacctc gcgatcgtct acaacaagaa
     1861 gctgttcgcc gacgccggtg tcgcgccgcc gactcccgac tggacctggg acgacttccg
     1921 cgcggcggcc gcgaaactga ccgatcccgc caagggccag ttcggctggc tgattcccgc
     1981 cgacggcagc gaagacaccg tctggcacta cattccgatg ctctgggagg ccggcggcaa
     2041 catcttgacc cctgacaaca cgaaggccgc gttcaactcc gaggccggtg tcaaagcgct
     2101 gacgactctg cagcagatgg cggtcgacga caagtcgctc tacctcgaca ccaccaacga
     2161 gaacgggccg aagctgatga acagcggcaa aatcgcgatg ttggtcaccg gcccgtggga
     2221 tctcagctcg ctgtccgaca tcgactacgg cgtgcaggtg atgcccacct acgccggctc
     2281 gaacggcgga ca
//
LOCUS       DASYLX010000962         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2253323,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000962 DASYLX010000000
VERSION     DASYLX010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253323"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..319)
                     /locus_tag="VGP27_22410"
     CDS             complement(8..319)
                     /locus_tag="VGP27_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7854083.1"
                     /translation="MTITGVITAILIGIVVGVLGRLLVPGKQPIGMLVTVLIGIVGAF
                     LGTAIARALGIPTVTNGIDWLELLVQVIVAALGVALVSAMMGRRRTGVTGGRRRSSLF
                     R"
     gene            442..1227
                     /locus_tag="VGP27_22415"
     CDS             442..1227
                     /locus_tag="VGP27_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854084.1"
                     /translation="MTDAKLALITGASSGIGLELAKLFAQNGYDLVVAAEDDGILGVP
                     QKLSEFGAEIHPVQVDLRTPEGVEHLYQSTIEGGRAIDVAALNAGAGKGGMFLETELS
                     DDLAVVDLNVRSTVHLAKLVLRDMANREQGKVLFTSSVASMMPGSYQTVYNASKSFIQ
                     SFAEALQDELRDTAITITSLMPGPTDTNFFARAGMLNTLVGKMPKDDPAQVARQGFEA
                     LMSGDKKVIGGSVPSKVMGTVSRVLPDTVKAVGSRLISKPRGS"
     gene            1880..2323
                     /locus_tag="VGP27_22420"
     CDS             1880..2323
                     /locus_tag="VGP27_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854085.1"
                     /translation="MSKETTPSPKDKRAVALGIVGAPTAAASPAPSSVESDYIGLLDA
                     SGMHIHGQDANELKLGYLLCALSQRNGIPPAGAAAYMTAARNSKLCYYVSTTGGPTGA
                     EVQQGLDLWQQQQNQPGIDAWNDTDQDNDGISDADDNAEYDPGHY"
     gene            2439..>2725
                     /locus_tag="VGP27_22425"
     CDS             2439..>2725
                     /locus_tag="VGP27_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEV7854086.1"
                     /translation="METDLLDVDTAKRRIVDLTDEVRSFCAGHRDGLCNVFVPHATAG
                     LAIIETGAGSDDDLVETLENLLPRDDRYRHAHGSPGHGADHVLPALVAPSVT"
BASE COUNT          561 a          845 c          836 g          483 t
ORIGIN      
        1 aatgcggtta ccggaacaag ctggaacgcc tgcgccctcc cgtgacgccg gtgcgcctgc
       61 gtcccatcat cgccgagacc agtgcgacac cgagggccgc gacgatcacc tgcaccagca
      121 gctccaacca gtcgatgccg ttggtcacag tggggatgcc gagtgctcgg gcgatggccg
      181 taccgaggaa ggcgccgacg atgccgatca gaaccgtcac gagcatgccg atgggctgct
      241 tgcccggtac cagcagccgg ccgagcacgc cgactacgat gccgatgagt attgcggtga
      301 taacgccggt gatggtcatc tctactcctc cagggcggta agtggtgcag aggaaatacc
      361 ctcggcctcg gagaaataaa cgccagcgtc aatccaccga gagttggttt ggtggtcatg
      421 tcggcgggta gccccgcgcg catgacggat gcaaagcttg ccctgatcac cggtgcctcc
      481 agtggaattg ggctcgaact ggccaaactg ttcgcacaga acggctacga cctggtcgtc
      541 gcggccgaag acgacggaat cctcggcgtg ccgcagaagc tgtctgagtt cggcgcggag
      601 attcatcccg tgcaggtgga cctgcggacc cccgagggcg tcgagcacct ctatcagagc
      661 accatcgagg gcggacgggc gatcgacgtg gccgccctca acgcgggagc gggtaaggga
      721 gggatgttcc tcgaaaccga gctctccgac gatctggctg ttgtcgacct gaacgttcgc
      781 tcgacggttc atcttgccaa gttggtgttg cgtgacatgg ccaaccgcga acagggcaag
      841 gtgttgttca cttcatcggt ggcatcgatg atgcccggct cgtaccaaac cgtttacaac
      901 gcgtcgaagt cgttcatcca gtcgttcgcc gaagcgctgc aggacgaact gcgcgatacc
      961 gccatcacga tcacgtcttt gatgccgggt cccaccgaca cgaacttctt cgcgcgcgcc
     1021 ggaatgttga acacgctcgt cggcaagatg cccaaggacg acccggcaca ggttgcgcgt
     1081 cagggattcg aggcgttgat gagcggtgac aagaaggtta tcggtggatc cgtgccgtcg
     1141 aaggtcatgg ggactgtgag ccgcgtactg cccgacacgg tgaaggcagt cggcagccgg
     1201 ttgatctcca agcctcgggg cagctaacgc atacccgtcg gcgtcggcgg cggccagcag
     1261 ctcagaacgt tctgatgatc attccgacta gtgccagcag cgcgtcgatg gcgaagaaaa
     1321 tcggcacgat ggcagggtca taaaagaact ggtcgagcgt gaacaacata cgcaatgatc
     1381 ccaacccagc acacttcaga caagcctttt cagcgtgtct aactgagcaa atcctagacg
     1441 tgggctattg atagttacat ttcacctccg cagcggagtc cgtggcagcg agtgcctgaa
     1501 gcggtcggca ggcgtggcgc cggtagagca catcgcggca ccgtcgtttc gcgagagctg
     1561 gccggcgcga ggcttacgga taacgaagct gtacgtttgc tgaagatcga attcgtttcg
     1621 tggatgaccg gctggtcgtg caatttgggt caattctggt cgccgcccgg ccgcaatata
     1681 tgcagttgaa ctggcatttc gcgggaaatt gacctcaacg ccgccaattt ctatttgccg
     1741 cgtgaagtaa cttgcgcaag tttgccatcg agatctgcgc cgtagctgtc tgtccattta
     1801 tgttcggctc accacgaggg tcctgagagc ggcctgtgca ccgggcacgc gctcagccga
     1861 caccatccgg aaggaccaca tgagcaaaga aacgacaccc tccccgaagg acaaacgcgc
     1921 cgtcgcgctg gggatcgtcg gcgcgccgac agccgcggcc agccctgccc cgtcgtctgt
     1981 cgaatccgac tacatcggtc tccttgacgc cagtggcatg cacatccacg ggcaggacgc
     2041 gaacgagctg aagcttggct atctgctctg tgcgctgagc cagcgcaatg ggataccacc
     2101 tgccggcgcc gcggcgtaca tgaccgccgc gagaaactcc aaactctgct actacgtgtc
     2161 cactaccggc ggaccgaccg gagccgaagt tcagcagggc ctcgacctat ggcagcaaca
     2221 gcagaatcag ccgggtatcg acgcctggaa cgacaccgat caggacaacg acggcatcag
     2281 cgatgccgac gacaacgcgg aatacgaccc aggtcactac tgagaaggcg aaataccaca
     2341 ggttttcggt cgatcgagcg gcgacgccta accgtcgagc gggttggctg cgactcgcgg
     2401 caactccctg gcggactcct gctcgtacgc tcgaattcat ggaaaccgat ctactcgacg
     2461 tcgacaccgc gaagcggcgc atcgtcgacc tgaccgacga agtccgttct ttctgcgccg
     2521 gtcaccggga tggcctctgc aacgtcttcg tgccgcatgc caccgcaggc ttggcgatca
     2581 tcgaaaccgg cgcgggctcc gacgacgacc tcgtcgaaac gctggagaac ctgctgcccc
     2641 gcgatgaccg ttaccgccac gcgcacggct cgccaggcca cggcgcagac catgtgctgc
     2701 ccgctcttgt ggcgccctcg gtgac
//
LOCUS       DASYLX010000963         4857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3462807,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000963 DASYLX010000000
VERSION     DASYLX010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462807"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..436
                     /gene="crgA"
                     /locus_tag="VGP27_22430"
     CDS             173..436
                     /gene="crgA"
                     /locus_tag="VGP27_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEV7854087.1"
                     /translation="MPKSKVRKKNDFTANSVSRTPVKVKAGPSSVWFVVLFLGLMVVG
                     LAWLLVFQLASTSIPFLADLGPWNYAIAFAFMITGLLLTMRWR"
     gene            592..1008
                     /locus_tag="VGP27_22435"
     CDS             592..1008
                     /locus_tag="VGP27_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7854088.1"
                     /translation="MQQTNWSPPAAGVAACGGIGLVMAIGAVTLVTDTPGRILAIIAA
                     AGLLLFATLSWRARPKLAISDGGLSIRGPLHTHRLAKSDIKIIRITEFRRIARKVRLL
                     EIDTIDDRLFVLTRWDLGADPLDVLDALTAAGLAGR"
     gene            complement(1027..1554)
                     /locus_tag="VGP27_22440"
     CDS             complement(1027..1554)
                     /locus_tag="VGP27_22440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7854089.1"
                     /translation="MTSPIQTATATLHTNRGDIKIALFGNHAPKTVANFVGLSEGSKD
                     YGTENAAGGTSGPFYDGAVFHRVIDGFMIQGGDPTGTGRGGPGYQFDDEFHPELQFDK
                     PYLLAMANAGPGTNGSQFFITVGKTPHLNRKHTIFGEVVDQESQKVVDGIASTTVDRS
                     DRPTEPVVIESITVS"
     gene            1661..2083
                     /gene="cwsA"
                     /locus_tag="VGP27_22445"
     CDS             1661..2083
                     /gene="cwsA"
                     /locus_tag="VGP27_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthesis protein CwsA"
                     /protein_id="HEV7854090.1"
                     /translation="MTLNTDARLTPGQRLSRGLRYTALGPVDLTRGTVGLSWQGAHSA
                     AGGLRQRFEKSRLAKEIEEAQGTIAGEIAAAQEVLANLPQIIADARKPKRRPRPLVLA
                     AAGVALIAVGGVVFSIVRRSMQPEPSPRPPSVDVTPKP"
     gene            2183..2719
                     /locus_tag="VGP27_22450"
     CDS             2183..2719
                     /locus_tag="VGP27_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV7854091.1"
                     /translation="MHSDTINGIPRVDLQARPLWKRNLSWALGGPLFASKKGLALWAR
                     VAAPVEAPLMKATGGRVRLAFSIPIVVLTSTGARSGQRRDIPLAYFTDGDDVVLIASN
                     SGGARHPAWYHNLLAHPDCELHIGQRGGSFMAREVEGPERDRLYELAVERLAKVFALH
                     EKRCGARTIPVMRLTPAA"
     regulatory      complement(2885..2999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            3204..3461
                     /locus_tag="VGP27_22455"
     CDS             3204..3461
                     /locus_tag="VGP27_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEV7854092.1"
                     /translation="MKAKVGDFLVVKGTTTERHDQHAEIIEVRSQDGSPPYVVRWLAT
                     DHEATVYPGCDAVVLTAAEHTQAAERASARARRMEAQLPVE"
     gene            complement(3897..4160)
                     /locus_tag="VGP27_22460"
     CDS             complement(3897..4160)
                     /locus_tag="VGP27_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854093.1"
                     /translation="MAETHRSGWAMNVLAAMAGVQALDFAQLSADRLGGDVQKWLDEL
                     TWWQPLRQGGIFLGQEATGVVLDSDELTRLIDIAREIRRDWWR"
     gene            complement(4239..4478)
                     /locus_tag="VGP27_22465"
     CDS             complement(4239..4478)
                     /locus_tag="VGP27_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854094.1"
                     /translation="MFCNALIFAPAWIAGDAAVCRNSWSLKPYTVVCSPAASTIVRGY
                     SSAAATACDHLSTARTATTMSTVPLTATVTQVVSV"
     gene            4390..4584
                     /locus_tag="VGP27_22470"
     CDS             4390..4584
                     /locus_tag="VGP27_22470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV7854095.1"
                     /translation="MYGFKLHELRHTAASPAIQAGANIKALQNMLGHESAGLTLEPLR
                     PPVRVGRRGGRCRDRRSFNS"
     gene            complement(4668..4740)
                     /locus_tag="VGP27_22475"
     tRNA            complement(4668..4740)
                     /locus_tag="VGP27_22475"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4705..4707),aa:Ala,seq:tgc)
BASE COUNT          839 a         1523 c         1569 g          926 t
ORIGIN      
        1 cccggccagg aggcagacca acggcacgcc gaagcgccac gccggccgtt ccgactctgc
       61 catcggctgc tcccagtcga attctctgcc tgaggcccgc ctacgttaat ctcttaggca
      121 gatcccgctt catcgtcgca ccaatggcgc cgactgtttt gtgaaggtac cgatgcccaa
      181 gtccaaggtc cgcaagaaga acgatttcac cgccaactcg gtgagccgca caccggtcaa
      241 ggtgaaggcc ggtccgtcga gcgtctggtt cgtggtgctg ttcctcggct tgatggtcgt
      301 cggccttgcc tggctgctgg tgttccagct ggcgtcgacg tcaattccat ttctggccga
      361 tctcggcccg tggaactacg cgatcgcctt tgctttcatg attaccggat tgttgctaac
      421 tatgcgttgg cggtgacagt cgcatcagtc ccaatgaatt tatcttggac ttggcgcgtt
      481 acccgttccg caacctgatg gtcacccaat cacatcgttg tgattcatcc ccattgggga
      541 tagcacttgt ggataaccct aaaaaccggg gtaaaagggt atggattgac cgtgcagcaa
      601 actaattgga gcccaccggc tgcgggcgtc gcagcgtgcg gcgggatcgg tctggtgatg
      661 gctatcggcg ctgtgaccct agtcacagac acccccggcc gcatactcgc aatcattgcc
      721 gctgccggac tccttttgtt tgcaaccctt tcctggcgcg cgcggccaaa gctggctatc
      781 agcgacggcg gtctatcgat tcgcgggccg ctgcatacgc accggcttgc taagagcgac
      841 atcaagatca tccggatcac tgaattccgg cgcatcgccc gaaaagtccg gctgctcgag
      901 atcgacacca tcgacgaccg gttattcgtc ctgaccaggt gggatctggg cgccgaccca
      961 ctcgacgtgc tcgacgccct gacggcggcg ggactggccg gccgctagtc gaccgctagg
     1021 agagcgtcag gagacggtga tcgactcgat caccaccggc tcggtcggcc ggtcgctgcg
     1081 gtccaccgtc gtggacgcga tgccgtcgac gaccttctgc gactcttgat cgacgacttc
     1141 accgaagatg gtgtgcttgc ggttcaggtg cggggtcttg ccgacggtga tgaagaactg
     1201 cgagccgttg gtgcccggtc ccgcattcgc catcgccagc agatacggct tgtcgaattg
     1261 cagctccggg tggaactcgt cgtcgaactg gtagcccggg ccgccgcggc cggttccggt
     1321 cgggtcgcca ccctggatca tgaagccgtc gatcacccgg tggaacaccg cgccgtcgta
     1381 gaacgggccg gaggtgccgc ccgcggcatt ctcggtgccg tagtccttgc tgccctccga
     1441 cagaccaacg aagttggcga cggttttggg cgcgtggttt ccgaacagtg cgatcttgat
     1501 gtcaccacga ttggtgtgca gggtcgcggt cgctgtctga atagggctcg tcacgagaac
     1561 tcagtctgcc acgcgcggtt gacgccgaac cgggcaccgt tgtgttgcag ttgatggcag
     1621 gctggtatcg attgcaacag ggccgcgaga gaggtgggag atgacgttga acacggatgc
     1681 ccggctgacc cccggacagc gattgtcgcg cggcctgcgc tacaccgcgc tgggtccggt
     1741 cgacctgacg cgaggcaccg tcgggctgag ctggcagggg gcacactccg cggcgggcgg
     1801 cctgcgtcag cgcttcgaga agagcaggct ggccaaggag atcgaagagg cgcaggggac
     1861 catcgccggt gaaatcgccg cggcgcagga ggtcctcgcg aaccttccgc agatcattgc
     1921 cgacgcacgc aagcccaagc ggcggccccg gcccttggta cttgcagccg ccggcgtggc
     1981 gctgattgcc gttgggggag tggtgttttc gattgtccgt aggtccatgc agcccgagcc
     2041 gtcgccgcgg ccaccgagcg tagacgtgac accgaagccg tagggtcgcg cagccgaagc
     2101 cgtcgcgact tctgcaaggc ggtcgtcgat tctgtcgaat tacggccttc gcgcagggtt
     2161 tcggcgtgaa ggtgggctca tcatgcacag cgatacgatc aacggaatcc ctcgcgtcga
     2221 cctgcaagcg cgtccgctgt ggaagcgcaa tctcagctgg gcgctgggcg gcccgctgtt
     2281 tgcaagcaag aaagggctcg ccttatgggc ccgcgtcgcg gcgcctgtgg aagctccgct
     2341 catgaaagcc acgggcgggc gcgtccgact cgcattctcg atccccattg tcgtgctgac
     2401 gtcgaccggg gcgcgcagcg gacagcgtcg cgatatcccg ctcgcatatt tcaccgacgg
     2461 tgacgacgtg gtgttgatcg catcgaattc cggcggcgcg cgacaccccg cctggtatca
     2521 caacctgctc gcccatcccg actgtgaact gcacatcggg cagcggggag gctccttcat
     2581 ggcccgtgaa gttgaaggcc cggaacgtga ccggttgtat gagctcgccg tcgaacgtct
     2641 cgcgaaggtg ttcgcgctac atgagaagcg ctgcggcgcc cggacgattc cggtcatgcg
     2701 gctgacgccg gcagcctaga gagaaaattg tggagcctag gggattcgaa cccccgactt
     2761 ctgcagtccg tcaccaggcc acccgaacaa gtcggctgtc atggcagcct ggtttgcgcc
     2821 gttgtcatcg ttggaatgcg cgttctcggt cattgcgcgt gccttactcg ggcctccacc
     2881 gcgggaggcg ctcttgccga caccgggttg gtgccaaccg cttcgttcat ccgagccacc
     2941 cagcgagcgt ggggttggcg tgccgtccag tcggagcttg gcgacggcaa ctcgtgaagc
     3001 aacttgattc tacgaggcgc ggcctaggca cgtgcacatt ttcatcggcg gtgtactcgc
     3061 agccgttagg gcaagcggcg cggtgtacgc cgtcgataac ggactcttgg actcaccttg
     3121 tcagatccgc ttccgccgag ttcgaatcgc gcgcacgatg gaggagtctg cgggtctcac
     3181 aaggtggagg aagggagcgg gcgatgaaag cgaaagtggg cgacttcctg gtggtcaagg
     3241 gcacgactac cgaacggcat gatcagcacg cggaaatcat cgaggtccgc tcgcaagacg
     3301 gctcgccgcc gtatgtggtg cgctggctcg cgaccgacca cgaggccacg gtgtatccgg
     3361 gctgcgatgc ggtggtgctc accgccgccg agcacaccca ggcggccgag cgggcgtcgg
     3421 cgcgggcgag acgtatggag gcccagcttc cagtggagta ggcggccaga aacaccagtt
     3481 cactttggtg gcgtcgtggt agtcgaggaa cgcaatggcg tcaaaggctt cacgcgatcg
     3541 gaggtcgcgc ctgcgtattc tcacagcacc ctcttggctt tgcggggcat gctgagacct
     3601 atgactgaac ctgaacatcg gactgagtcg acaactgtgg ctcaggagcc aagtgccgcc
     3661 gccgaatcgc catacgggcg ggaccggtgg caccgacgtg gcaggccatt tcggtttgcg
     3721 gctatggtcg tcgccttggc cggcatcgtt ttcatcatcg ccgtgatctt ttggagcgga
     3781 ttcgtcctgg gagcctgcag cggtggccat cacgggcatc actgggacgg ttcccgtcac
     3841 tcatcgatgt cttcgctcta cgcgccggag ttggcttcag cgggcacttt caccgcctag
     3901 cgccaccaat cgcgccggat ctctcgcgct atgtcgatta gccgcgtcag ttcgtcgctg
     3961 tcaagcacca cacctgtcgc ttcttggccc aggaagatgc cgccttggcg tagcggttgc
     4021 caccacgtca actcgtcgag ccacttctgg acatcgccgc cgagacggtc cgcggacagc
     4081 tgggcgaagt cgagggcctg tacccccgcc atcgctgcca agacgttcat ggcccatccg
     4141 ctgcggtggg tctccgccat cagcgcgccg agtgttcttg gcgggtgccg caggccgcag
     4201 aggcttcacg tcacgagcgt ccggcgccca cagctccatc agacggacac cacctgcgtc
     4261 accgtcgccg tcaggggcac cgtgctcatc gtcgtggctg tcctggccgt gctgaggtgg
     4321 tcgcaagccg tcgcggccgc cgagctgtac ccgcggacga tcgtcgacgc ggccggtgag
     4381 caaactaccg tgtacggctt caagctccac gagttgcggc acaccgcggc gtcgccggcg
     4441 atccaggcgg gcgcgaatat caaggcgttg cagaacatgc tcggacacga gtcggcgggc
     4501 ctgacgctgg aaccgctacg gccacctgta cgggtcggac gtcgaggcgg tcggtgtcgc
     4561 gatcgacgat cttttaactc gtgattgtgg gccaaatgag ggcgcggacc ttcttaggac
     4621 gatgcctgaa aaggtgcgag ataccgcttg agcagcggtt tttgttgtgg agcctagggg
     4681 attcgaaccc ctgacttctg ccttgcaaag gcagcgctct accaactgag ctaaggcccc
     4741 gtttcaggaa ggttggtcgg cagctgcgtg ccacacctcc acgccgtgtc gcgaccgcac
     4801 cacgaaaacc gccaacgcca ccgccgcgac cagtaacacc agcctcatgg tgaccct
//
LOCUS       DASYLX010000964        10725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2146494,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000964 DASYLX010000000
VERSION     DASYLX010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146494"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2782)
                     /locus_tag="VGP27_22480"
     CDS             complement(134..2782)
                     /locus_tag="VGP27_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV7854096.1"
                     /translation="MPAPSGVVTFLFTDIEGSTRRWESDAGTMRSALAAHDAVLRRAI
                     ESHGGFLFKHTGDGVIAAFASPTSAVDAAIAAQRELDLPVRMGIATGEAALADGDYFG
                     AVLNRAARVMTAGHGGQILVADSTASLVSGLDLADLGPRRLRDVPTPVGLFQVRAPGL
                     REEFPPLRALDTSPGNLRQATTSFIGRDAEVAEIKDAVRTHRLITLTGVGGVGKTRLA
                     LEAATQLGDEFPDGIWLFELAAVTDPAAVPDAVAAVLGITQQPAKTLTESVAIALEGR
                     VRLLVLDNCEHVLDAAADLIEAVLRQSTTVKVLATSREGLGVANEQAWRVRSLDADTA
                     VDLFIERARAVAQAFSADQPEAISEICRRLDGIPLAVELAASRMASMTAGEVRDRLDH
                     RFRLLVGARRGLERHQTLRHAVQWSYDLLDEPERKLLEICSVFTGGFSLESVCAVTGS
                     DDDFVTLDLLDALVRKSLIAADRSSDHTRFSMLETIRQFAEEKLVARGGASRLRTAHA
                     RYFAGREAIMMSVWDGPRQPEAYYWFSTELANLRSGFRWSADQGDLDAATAIVNVAGW
                     LGVLVENVEPITWAEELFESARQEDHPRLAAMCGIASLCFLFGRVDDALYYSQVGQEA
                     LSRRPARLRFGAEQLWLGAVYSAIPGSDRYVAFYQQQLEHSVDIYGLGQASLVTALRW
                     SGRIDEAISAASGLTAVAEASLNPFVASFALMSYGMACSAHNPIRALDALRKGAKIAQ
                     ETGNRSNESYIAMTIAMTLNRIEGEAADHLSSLDYLTLAIRNYHDSGNLTQLRASLGL
                     LATFLDSRGKVVSAATIAGFASVLPTAAPSVPEFEAGITHLRDALTAEIYEALAREGG
                     AMSMAEIVAYAYDQIDHVRAELEQLP"
     gene            complement(2866..3843)
                     /gene="whiA"
                     /locus_tag="VGP27_22485"
     CDS             complement(2866..3843)
                     /gene="whiA"
                     /locus_tag="VGP27_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV7854097.1"
                     /translation="MTAEVKDELSRLVVNSVSARRAEVASLLRFAGGLHIVSGRVVVE
                     AEVDLGIIARRLRKDIFDLYGYNAVVHVLSASGIRKSTRYVVRVAKDGEALARQTGLL
                     DLRGRPVRGLPAQVVGGSVADAEAAWRGAFLAHGSLTEPGRSSALEVSCPGPEAALAL
                     VGAARRLGVSAKAREVRGADRVVVRDGEAIGALLTRMGAQDTRLTWEERRMRREVRAT
                     ANRLANFDDANLRRSARAAVAAAARVERALAILGDSVPDHLAAAGKLRVEHRQASLEE
                     LGRLADPVMTKDAVAGRIRRLLSMADRKAKQEGIPDTESAVTPDLLDDA"
     gene            complement(3840..4871)
                     /gene="yvcK"
                     /locus_tag="VGP27_22490"
     CDS             complement(3840..4871)
                     /gene="yvcK"
                     /locus_tag="VGP27_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEV7854098.1"
                     /translation="MNSRVVALGGGHGLYATLSAARRLTPHVTAVVTVADDGGSSGRL
                     RSELDVVPPGDLRMALAALASDSPHGRLWATILQHRFGGSGALAGHPIGNLLMAGLNE
                     VLADPVAALDELGRILGVKGRVLPMCPIALQIEADVAGLESDPRMSRVIRGQVAIATT
                     PGKVRRVRLLPSDPPATRQAVDAIMNADLVVLGPGSWFTSVIPHVLVPELTAALQATS
                     ARRALVLNLVAEPGETAGFSVERHIHVLSQHAPGFTVNEIVVDASRVPGEREREQISR
                     TATILGAAVQFAEVSRPGTPLHDPARLAAALEGVRLRGTGPGGNLQQPTTSAPARTVY
                     GPRGDDQWR"
     gene            complement(4868..5800)
                     /gene="rapZ"
                     /locus_tag="VGP27_22495"
     CDS             complement(4868..5800)
                     /gene="rapZ"
                     /locus_tag="VGP27_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV7854099.1"
                     /translation="MTDQGAGDDLRGQDDATRGTGTESGIDVVLVTGLSGAGRGTAAK
                     VLEDLGWYVADNLPPELIARMVDLGLAAGSRITQLAVVMDVRSRGFTGDLDWVRKDLA
                     TRDIAPRVLFLEASDDILVRRYEQNRRSHPLQGNQTLAEGIAAERAMLAPVRATADLI
                     IDTSTLSVPALRESIESAFGGETVAYTSVTVESFGYKYGLPMDADTVMDVRFLPNPHW
                     VDELRRHTGQHPAVREYVLGQPGAAEFLETYHRLLDLVIEGYRREGKRYMTVAIGCTG
                     GKHRSVAMAEALAGRLQGGDQLTVRVLHRDLGRE"
     gene            complement(5797..7779)
                     /gene="uvrC"
                     /locus_tag="VGP27_22500"
     CDS             complement(5797..7779)
                     /gene="uvrC"
                     /locus_tag="VGP27_22500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV7854100.1"
                     /translation="MPDPATYRPAPGSIPVEPGVYRFRDPHGRVIYVGKAKSLRSRLN
                     SYFADISGLAPRTRQMVMSAASVEWTVVSTEVEALQLEYNWIKEFDPRFNIRYRDDKS
                     YPVLAVTLNEEYPRLMVYRGPRRKGVRYFGPYSHAWAIRETLDLLTRVFPARTCSAGV
                     FKRHKQIDRPCLLGYIDKCSAPCIGRVSAEEHRKIVLDFCDFLAGKTDRLARDMEREM
                     NKAAEQLDFERAARLRDNIGALKRALEKQTVVFGDGTDADVVAFADDDLEAAVQVFHV
                     RGGRVRGQRGWIVEKSGDPEDSGQGHLVEQFLTQFYGDQAELGGAADESTNPVPRQIL
                     VPVLPPNADELETWLCGLRGSRVALRVPRRGDKRALAETVKRNAQGALAQHKLKRAGD
                     FTARSAALQSIQEALGLADAPLRIECIDISHVQGTDVVASLVVFEDGLPRKSDYRHYA
                     IREAAGGGRSDDVASIAEVTRRRFARHVSDLQHTSVLTAEGKSRKFAYPPNLYVVDGG
                     APQVNAAAAVLDELGVSDVAVIGLAKRLEEVWVPSEPDPVIMPRNSEGLFLLQRVRDE
                     AHRFAITYHRSKRSKRMTASALDSVPGLGEHRRKALVTHFGSVARLKQASVEEITTVP
                     GIGLTTARAVLEALGAAAESSAPASAIGNDQTRASG"
     gene            7815..9752
                     /locus_tag="VGP27_22505"
     CDS             7815..9752
                     /locus_tag="VGP27_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV7854101.1"
                     /translation="MLYRRDVRTPSLQKLAAPLAGVVLVLAGCGAEDHAPTSAAGPCA
                     IVENGSPAPKTSQAAGSQPELATGYRKDMTAVRTGHYAVATANPLATQKACEVLKEGG
                     TAADALVAAQAVLGLVEPQSSGIGGGGFLLYYDAAANSVQAYDGRETAPAAAGENYLR
                     WISDTDRTEPKPDARASGRSIGVPGILRMLMDAHRQHGKAAWRDLFAPAVALADDGFD
                     ISPRLAAAIADAAPQLKLDREAAAYFLDPDGGPKAAGTKLTNPAYSKTLGVIASDPQS
                     FYTGDIAKAVVAAATDTSGGRTPSLMTTEDLAGYTAKQREPICMPYRGKEICGMPPPS
                     SGGIAVAATLGILEHFPMSDHKPTDMDRNGGRPTAMGVHLIAEAERLAYADRDKYVAD
                     TDFVPLPGGTPNTLLNSNYLAQRAALVSLQRSMGTAKPGEFGASPSTPVPVPEHGTSQ
                     ISVVDSQGNAASLTTTVESQFGSFHMVDGFILNNQLTDFSAEPVGPDGVPLANRVEPG
                     KRPRSTMAPTLIFEKSGAARGPLFATLGSPGGAVIIQFVVKTVVGMLDWGMDPQQAVG
                     MVDFGAANSPKTNVGGEHPDIDSTDNGDHDPLVQGLRELGHQVDLADQSSGLSAIVRD
                     SPGWTGGADPRREGLVMGDPR"
     gene            9749..10216
                     /locus_tag="VGP27_22510"
     CDS             9749..10216
                     /locus_tag="VGP27_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7854102.1"
                     /translation="MTALVTRLTESDWRMFAGVRLRALTDSLGADDPQYRQESTFTAA
                     QWRRRLRDHAQFVALLNDRAVGLIGAQQEDAETVYLYSLWLDPAARGHGLARPLVAAA
                     VEWARDQRARLVTLRVAADNTVARGVYQSLGFTEVAGERADARHEVGMSLTVQ"
     gene            complement(10203..10658)
                     /locus_tag="VGP27_22515"
     CDS             complement(10203..10658)
                     /locus_tag="VGP27_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV7854103.1"
                     /translation="MSDGWDLDVRPYLTPFFAYGAAALIVAAHVTVGVLLKISSTGVI
                     FQTADQVAIALLGVVIAGAVLMFARPRLRAGASGVAVRNLLGYRLIPWADVVGVSFPV
                     GARWARIDLPDDEYIPVMAIQAVDKERAVEAMDDVRGLLARYRPDLTAP"
BASE COUNT         1853 a         3720 c         3404 g         1748 t
ORIGIN      
        1 gatgaattcg acgcgcatac ccagcttgtc ggcgaccgcg gtcatcaggt cgacgtcaag
       61 tccaccgccg tccggcatgc cgttgaatgg aggatcgggg taggccgcgc cgactctcag
      121 tgtctgcatg cgctcacggt agttgttcga gctccgctcg gacgtgatcg atttggtcgt
      181 acgcataggc gacgatctcg gccatggaca tggccccgcc ctctcgagcc agcgcctcgt
      241 aaatctctgc ggtcagggcg tcgcggagat gggtgattcc tgcttcgaat tccggcacac
      301 tcggtgccgc cgtcggaagg accgacgcga aacccgcgat agtagcggcc gataccactt
      361 tgccgcgact atcgagaaat gtcgccagca gacccagcga cgcacgaagc tgagtgaggt
      421 tgcccgagtc gtggtagttg cgaatcgcca aggtcaggta gtcgagcgag gagaggtggt
      481 ccgcggcttc gccttcgatc cgattcagag tcatggcgat tgtcattgcg atataggact
      541 cattgcttcg gttacccgtc tcctgagcga tcttcgcgcc tttccgaagc gcgtcgagtg
      601 cccgaattgg attatgcgcg ctgcacgcca tcccgtagct catcaaagcg aaggaagcga
      661 caaaaggatt gaggctggcc tcggccacgg cggtcaggcc agacgcagca gagatcgcct
      721 catcaattcg accgctccac ctcaaggcag taaccagaga agcctggccg aggccatata
      781 tgtccacact gtgctcgagt tgttgctggt aaaacgcgac atatcgatca gacccgggga
      841 tggctgagta cacagcgccg agccatagtt gttcagcgcc gaatcgcaac cgtgccgggc
      901 gtcgacttaa cgcctcttga ccgacttggc tgtagtagag cgcgtcgtcg acgcggccga
      961 acaagaaaca caaagacgcg atcccgcaca tagccgcgag ccttggatga tcctcctgac
     1021 gagccgattc aaaaagttct tcggcccaag tgatcggctc gacattctcg acgagaacac
     1081 ctagccagcc cgccacgttc acgatggcag ttgcggcgtc aagatcacct tggtcggcgg
     1141 accaccgaaa cccactgcgc aggttcgcca gttcagttga gaaccagtaa taagcctccg
     1201 gttgccgagg accgtcccac acgctcatca tgatcgcttc acgcccggcg aagtagcggg
     1261 cgtgcgcggt acgcagcctg ctggctcctc cgcgagcgac cagcttctcc tcggcgaatt
     1321 ggcggatcgt ctccaacatt gagaagcggg tgtgatccga tgaccggtcg gccgcgatca
     1381 gcgacttgcg gacgagcgcg tccaacaggt cgagggtgac gaaatcgtcg tcggaacctg
     1441 ttacagcgca gacgctttcg aggctgaagc cgccggtgaa aaccgaacag atctccagta
     1501 gctttctctc cggctcatcg agcaggtcgt acgaccattg caccgcgtgc cgcagtgtct
     1561 gatggcgctc caggccgcgc cgtgcaccca ccagcagccg gaaccggtgg tcgagtctgt
     1621 cccgcacctc ccctgcggtc atcgaggcca tccgggacgc cgccaactcc acggccaacg
     1681 ggatgccatc gagacgacgg cagatctccg agatcgcctc cggctgatcg gctgaaaacg
     1741 cctgtgccac cgcgcgggca cgctcgatga acagatccac agccgtgtcg gcgtccagcg
     1801 atcgcacgcg ccatgcctgt tcgttcgcaa ctcccagtcc ttcgcggctg gtggccagga
     1861 ccttgactgt cgtcgactgc ctcaagacag cttcgatcag atcggccgcc gcgtcgagga
     1921 cgtgttcgca attgtccaat accaacaacc ggactctgcc ctcgagcgct atcgcgaccg
     1981 actcggtcaa ggtcttcgca ggttgttgtg tgatgccgag gaccgcagcg accgcatcag
     2041 gaaccgcagc cggatccgtc actgctgcca attcgaaaag ccatatgccg tctggaaatt
     2101 cgtcacccaa ctgggtggcg gcttccaggg ccagtcgggt cttgccgacg ccgccgacac
     2161 cggtcagggt aatcagccga tgagtgcgga cggcgtcttt gatctcggcg acttccgcat
     2221 cgcgtccgat gaagcttgtc gtcgcctgcc gtaggttgcc cgggctggtg tcgagtgcgc
     2281 gcagcggcgg gaactcctcg cgtagacctg gtgcgcggac ttgaaacaag ccgaccggtg
     2341 tcggtacatc gcgaagtcgc cgggggccca gatcggcaag gtccaggcca ctgaccaagg
     2401 aagccgtcga gtcggccacc aagatctgtc cgccatgccc tgccgtcatc acccgggccg
     2461 cgcggttgag caccgcgccg aagtaatcgc cgtccgcaag cgccgcctca ccggtggcta
     2521 tccccatccg caccggcaaa tccaactctc gctgcgccgc aatcgccgca tccactgcgg
     2581 acgtgggcga tgcaaacgcc gcgatcacac cgtcaccggt gtgcttgaac aggaaaccgc
     2641 cgtgcgattc gatcgctctg cgcagaacgg catcgtgtgc ggcaagcgcc gagcgcatcg
     2701 tgcccgcgtc ggactcccac cgacgggtcg aaccctcgat gtcggtgaac aggaacgtca
     2761 ccaccccgga tggagccggc attcgcacag gatcgcacaa aagattgcgc ctatggtcgt
     2821 gctcgggcac gaaatcacga ccgcaagtgc aatttcaagc gtgtgctagg cgtcgtcgag
     2881 cagatccggg gtgacggccg actcggtgtc gggaattccc tcttgtttgg ccttgcggtc
     2941 ggccatcgac agcagccgcc gaatacggcc tgccacagcg tctttcgtca tcaccgggtc
     3001 ggccagcctg ccgagctcct cgagcgacgc ctgccggtgt tcgacccgca atttgccggc
     3061 ggcggcgagg tggtccggaa cggagtcgcc gaggatggcc aacgcccgct cgacccgggc
     3121 ggcagccgcc accgcggccc tggccgatcg ccgcaggttc gcgtcgtcga agttcgccag
     3181 ccggttcgcg gtcgctcgca cctcgcggcg catccggcgc tcttcccacg tcagcctggt
     3241 gtcctgggcg cccatcctgg tcagcaacgc gccgatggcc tctccgtcgc gcacgacgac
     3301 gcggtcggcg ccgcggacct cgcgcgcctt ggcgctcaca ccgagccgtc gcgcggcgcc
     3361 gaccagggcc agcgcggcct ctggtcccgg gcaactgact tccagtgcgg atgaccggcc
     3421 cggttctgtc agcgaaccgt gagccaaaaa cgcgccgcgc caggccgcct ccgcgtcggc
     3481 cacgctgccg ccgaccacct gtgcgggcag accgcgcacc ggacggccac ggagatcaag
     3541 cagtccggtc tggcgcgcca gcgcttcgcc gtccttggcg acacggacca cataccgcgt
     3601 gctcttgcga atcccgctgg cggacaacac atgaacgacg gcgttgtacc cgtacaggtc
     3661 gaagatgtcc ttccgaagcc gacgcgcgat gatcccgaga tccacctcgg cctcgaccac
     3721 gacacggcca gaaacgatgt gcaggcctcc ggcgaaccgc agcaacgacg ccacctcggc
     3781 acggcgcgcg ctgaccgaat tgacaacaag acggctcagc tcgtccttga cctcggctgt
     3841 catcgccact ggtcgtcacc tctcggtccg taaactgttc gggcgggtgc ggaggtcgtc
     3901 ggctgctgga gattcccacc gggtcccgtc ccacgcagac ggaccccctc caacgccgcg
     3961 gccaacctcg ccgggtcatg taaaggtgta ccaggtcgag acacctcagc aaactgaact
     4021 gcagcaccca gtatcgtggc ggtccgactg atctgctcgc gctcccgctc accaggcact
     4081 cgagatgcgt cgacgacgat ctcgttcacc gtgaaacccg gcgcatgctg ggacagcaca
     4141 tggatatgcc gttcgaccga gaaacccgcc gtctcccccg gctctgccac caggttcagc
     4201 accagtgcac gtcgtgcgga ggtggcttgc aacgcggccg tcagctccgg caccaggacg
     4261 tgcggaatca cactggtgaa ccacgagccg gggcccagca ccacgaggtc ggcgttcatg
     4321 atggcgtcca ccgcctggcg agtggccggc gggtcgctgg gcagcagccg caccctgcgc
     4381 accttgcccg gcgtggtggc gatcgccacc tggccgcgga tcacccggct cattcgcgga
     4441 tccgattcga gccctgcgac atccgcctcg atctgcagtg cgatcgggca catcggcagc
     4501 acccggccct tgacaccgag aatgcggccc aattcgtcaa gcgctgcaac gggatcggcg
     4561 agcacttcgt tgaggcccgc catcaacagg ttgccgatgg gatggcctgc cagcgcgccg
     4621 ctgccgccga acctgtgctg aagaatcgtc gcccacagcc ggccgtgcgg gctgtcggac
     4681 gccagagccg ccaatgccat tcgcagatcg ccgggcggca ccacgtcgag ctcgctgcgc
     4741 agccgccccg acgaaccgcc gtcatcggca accgtgacca cggcggtgac atgcggggtg
     4801 agcctgcggg cggccgacag cgtggcatag agcccgtggc cgccgcccag tgcgacgacg
     4861 cgcgaattca ttcgcggccc aggtctcgat gcagcactcg caccgtgagt tggtcaccgc
     4921 cctgcagtcg tccggccagc gcctctgcca tcgcgacgct gcgatgcttg ccgccggtgc
     4981 agccgatggc aacggtcatg tagcgcttcc cctcccggcg gtagccctcg atgacgaggt
     5041 ccagtagtcg atggtaggtc tcgagaaact ccgccgcgcc cggctgcccc aacacgtact
     5101 cgcgcacggc gggatgctgc ccggtgtgcc tgcggagttc gtccacccag tgcgggttcg
     5161 gcaggaaccg gacatccatc accgtgtcgg catccatcgg taatccgtac ttgtagccga
     5221 aggactccac ggtcacgctg gtgtaggcga cggtctcgcc gccgaacgcg ctctcgatgc
     5281 tctcccgcag cgcaggtacg gacaacgtcg aggtgtcgat gatcaggtcg gcggtcgccc
     5341 gcaccggcgc cagcatggcg cgctcggcgg cgatgccctc ggcgagtgtc tggttgccct
     5401 gcagcgggtg gctgcgccgg ttctgctcgt agcgccgcac cagaatgtcg tcggaggcct
     5461 ccaagaacaa gactcgcggt gcgatgtcac gcgtcgccaa atccttgcgc acccagtcca
     5521 agtcgccggt gaagccacgc gatcgcacgt ccatcaccac ggcgagctgg gtgattcgcg
     5581 agcccgccgc aagacccagg tcgaccatgc gggcgatgag ctcgggcggc agattgtctg
     5641 cgacatacca cccgaggtct tcgagcacct tggccgcggt gccgcgaccc gcaccggaaa
     5701 ggcccgtgac gaggaccacg tcgatgcccg attccgtgcc cgtaccgcgg gtcgcatcgt
     5761 cctgcccccg caaatcgtca cccgcgccct ggtccgtcat cccgatgccc tggtctgatc
     5821 attgccgatc gccgaggcgg gcgctgacga ttcggctgct gcgcccagcg cctccaggac
     5881 ggctcttgcg gtcgtcagac cgatgcccgg cacagtggtg atctcctcga cgctggcctg
     5941 cttcagccgg gccaccgacc cgaagtgcgt gaccagcgcc tttcgccggt gctcccccaa
     6001 cccgggcacc gagtccagcg ccgacgccgt catccgcttg gaccgcttgc tgcggtggta
     6061 ggtgatcgcg aagcggtgcg cctcgtctcg cacccgctgc aacagaaaaa gcccttcgct
     6121 gttgcggggc atgatcaccg ggtcgggttc cgacggcacc cacacctcct ccagccgctt
     6181 ggccagcccg atgacggcca cgtcggagac gccgagctcg tcgaggactg ccgccgccgc
     6241 gttgacctgc ggagcgccgc cgtcgaccac atacaggttc ggcggatagg cgaacttgcg
     6301 cgacttgcct tccgcggtaa gcacactggt gtgctgcaga tcggatacgt gccgcgcgaa
     6361 ccgacgccgc gtcacctccg cgatggatgc cacgtcgtcg gagcgcccgc cgcctgccgc
     6421 ctcccggatc gcgtagtggc ggtagtcgga tttgcgtggc aggccgtcct cgaacaccac
     6481 gagtgacgcc accacatcgg tgccctggac gtggctgatg tcgatgcatt cgatgcgcag
     6541 cggcgcatcg gccagcccga gagcctcctg aatgctttgc agtgcagcag atctcgcggt
     6601 gaagtcgcct gcccgcttga gcttgtgctg cgccagcgca ccttgcgcgt tgcgtttgac
     6661 cgtttcggcg agcgcccgct tgtcgccgcg cctcggcacc cgaagcgcca cccgtgatcc
     6721 gcgcagcccg cacagccagg tctcgagttc gtcggcgttg ggcggcagca ccggaaccag
     6781 gatctgacgt ggcaccgggt tggtcgactc gtcggccgcg ccgcccaact cagcctgatc
     6841 cccgtagaac tgggtgagga actgctcgac caggtgccct tggccggaat cctcgggatc
     6901 gccggacttt tcgactatcc agccgcgctg gccgcgcact cgcccgccgc gaacgtggaa
     6961 cacctgcacg gccgcctcga ggtcgtcgtc ggcgaaggcc accacgtctg cgtcggtgcc
     7021 gtcgccgaag acgaccgtct gcttctccag tgctcgtttg agggcgccga tgttgtcccg
     7081 cagccgtgcc gcccgctcga agtccagctg ctcggcggcc ttgttcatct cgcgttccat
     7141 atcgcgcgcc agccggtcgg tcttgcctgc caggaagtcg cagaagtcga gcacgatctt
     7201 cctgtgttct tctgcggaca ccctcccgat gcacggcgcc gagcacttgt cgatgtatcc
     7261 caacaggcac ggccggtcga tctgcttgtg gcgcttgaac actccagccg aacacgttcg
     7321 agccggaaac acccgggtga gcagatcgag tgtctcgcgg atggcccagg catgcgagta
     7381 gggcccgaag taccggacgc ccttgcggcg cggcccgcgg tagaccatca gccgcggata
     7441 ctcctcgttg agcgtgacgg ccagcaccgg gtaggacttg tcgtcgcggt aacggatgtt
     7501 gaaccgcgga tcgaactcct tgatccagtt gtattcgagc tgcagggcct cgacttcggt
     7561 ggacaccacc gtccactcga cgctggccgc ggacatcacc atctggcgcg tccggggcgc
     7621 caacccggag atatccgcga agtaggagtt cagccggctg cgcaggctct tggccttgcc
     7681 gacgtagatg acccggccgt gcggatcgcg gaaccggtaa acgccgggtt cgacggggat
     7741 cgaccctggc gcgggtcggt acgtcgctgg atcgggcacg gattccaggt tactgccggt
     7801 gtcggacgga cctgatgctc taccgtcgag atgtgcggac accgtcactg cagaagctgg
     7861 cggccccgct ggcgggggtc gtcttggtgc tggccggatg cggcgccgag gaccatgcac
     7921 caacctcggc ggccggtccg tgcgccatcg tcgagaacgg cagcccggcg ccgaagacct
     7981 cgcaggccgc aggctcgcaa cccgagctgg ccaccggtta ccgcaaggac atgaccgcgg
     8041 tgcgcaccgg gcactatgcg gtcgccacgg ccaatccgct ggccactcag aaggcctgcg
     8101 aggtgctcaa ggagggcggc acggcggccg atgcgctggt cgcagctcag gccgtgctgg
     8161 gcctggtgga gccacagtcg tccgggatcg gcggcggcgg gttcctgctc tactacgacg
     8221 ccgcggccaa ctccgtgcag gcctacgacg gcagggagac cgctcccgcc gcggcaggcg
     8281 agaactacct gcgctggatc tccgacaccg atcgcaccga gcccaagccg gatgcccggg
     8341 cgtccggccg ctcgatcggc gtgcccggca tcctgcgcat gctgatggat gctcaccgtc
     8401 agcacggcaa ggccgcgtgg cgcgacctct tcgctcccgc cgtggcgctg gccgacgacg
     8461 gcttcgacat cagcccccgg ttggccgctg ccatcgccga cgccgcacca cagctgaagc
     8521 tcgacaggga ggctgccgcc tacttcctcg atcccgacgg cggaccgaag gccgccggca
     8581 ccaagctcac caacccggcg tactcaaaga cgttgggcgt catcgcatcc gacccgcaat
     8641 cgttttacac cggcgatatc gccaaagccg tcgtcgcggc ggccaccgat acatcgggcg
     8701 gccgcacacc gagcctgatg acgaccgagg atctggccgg ctacaccgcc aaacagcgcg
     8761 agccgatctg catgccctat cgcggcaagg agatctgcgg catgccgcca ccctcgtcgg
     8821 gtggcatcgc ggtcgccgcg acactcggca tcctcgagca ctttccgatg agcgaccaca
     8881 aacccactga tatggaccgc aacggcggca gaccgacggc aatgggggtg cacctcatcg
     8941 cggaggccga gcgcctggcg tacgccgacc gggacaagta cgtcgccgac acggatttcg
     9001 tgccgttgcc aggtggaaca ccgaacacac tattgaacag caactacctg gcccagcggg
     9061 ccgcgctcgt ctccctgcag cgcagcatgg gcaccgcgaa acccggcgaa ttcggggcgt
     9121 cgccgtcgac gccagtgccc gtgcctgaac acggcaccag ccagatcagc gtcgtggact
     9181 cgcagggcaa cgccgcttct ttgaccacca ccgtcgagtc gcagttcggc tcgttccaca
     9241 tggtcgacgg tttcatcctg aacaatcaac tgaccgactt ctccgccgaa cccgtcggcc
     9301 cggacggggt gccgctggcc aaccgggtcg agccgggcaa gcggccgcga agcacgatgg
     9361 cgccaactct gatcttcgag aagtcaggtg ccgcccgcgg tccactgttc gcgacgctgg
     9421 gatcgcctgg cggtgcggtc atcattcagt tcgtcgtcaa aactgtcgtc ggaatgctcg
     9481 attggggtat ggatccgcag caggcggtgg gcatggtcga cttcggggcg gccaactctc
     9541 ccaaaaccaa tgtcggcggc gagcatcccg acatcgacag caccgacaac ggagaccacg
     9601 acccactcgt gcagggcctg cgcgaactcg gccatcaagt cgacctcgcc gaccagtcca
     9661 gcgggttgtc ggcgatcgtg cgcgactcgc ccggctggac cggcggggcg gacccgcgcc
     9721 gcgaggggct ggtgatgggc gaccctcgtt gaccgcactg gtcacccggc tgacggagtc
     9781 ggactggcgg atgttcgccg gtgtccgtct gcgtgcgctc accgattcac tcggtgccga
     9841 cgacccgcag taccggcagg aatccacgtt caccgccgcg cagtggcgca ggcggctccg
     9901 cgaccatgcg cagttcgtcg cgctgttgaa cgatcgcgcg gtgggactga tcggtgcaca
     9961 gcaggaggat gccgaaacgg tttacctgta ttcactttgg ctcgatccgg ccgcccgcgg
    10021 gcacggcctg gctcgaccgc tggtggctgc cgccgtggag tgggcacgcg atcagcgagc
    10081 gcgcctcgtg accctgcggg tggcagccga caacaccgtg gcgcgagggg tataccaaag
    10141 cctcggcttc accgaagtgg ccggcgaacg cgccgacgcc cgccacgagg tcgggatgtc
    10201 gcttacggtg cagtgaggtc gggacgatag cgggccagca ggccgcgcac gtcgtccatc
    10261 gcctccacgg cacgctcctt gtcgacggcc tgaatcgcca ttaccgggat gtactcatcg
    10321 tcgggcaggt cgatacgagc ccagcgcgcg ccgaccggaa agctgacgcc gacaacatca
    10381 gcccacggaa tcagccggta gccaagcaga ttccgcaccg cgacgcccga cgcgccggcg
    10441 cgcaaccgcg gccgcgcgaa catcagcacg gcgccggcga tgacaacgcc gagcagggcg
    10501 attgccacct gatctgcggt ctggaagatg acgcccgtag acgagatctt cagtagcaca
    10561 ccgacggtca cgtgggccgc gacgatcaac gccgcggcgc cgtaggcgaa aaacggtgtc
    10621 agataggggc gtacgtcgag atcccagccg tcgctcatga ccgcttgcgg tcgttctctt
    10681 tgctcctcgc gtgcgcgaag tcctctttgc tcctcgcgtg cgcga
//
LOCUS       DASYLX010000965         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2365604,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000965 DASYLX010000000
VERSION     DASYLX010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365604"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VGP27_22520"
     CDS             <1..643
                     /locus_tag="VGP27_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854104.1"
                     /translation="GSAGADVVPVQADLRRPEEVQRLYRTATEGGRHLTAAALNAGTG
                     GAGKFVERDLESDLDVVDLNVRSTVLLSKLVLQDMAAQGSGKVLYTSSIVAQMPGSHQ
                     AMYAASKSFVQSFAEALHDEMRDTGVTVTSLMPGPTDTNFFARLGMADTVVGRMPKDD
                     PGEVAKQGYDAMMRGEQKVVAASPMSKAMGLVNRFLPDSVKAVAHRLISQPPP"
     gene            640..>1866
                     /locus_tag="VGP27_22525"
     CDS             640..>1866
                     /locus_tag="VGP27_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854105.1"
                     /translation="MIRQTADAVVIGAGHNGLVAAAMLADAGWDVLVLEAQPEPGGAV
                     KSAELFPGFVSDLYSAFYPLSVVSPALRALNLEDHGLQWTHAPGVVGHARSADDDDAP
                     VIYRDIDRTIADLNRRDPGDGDQWGELFEQWQRISQPLLSTLFSPFPPLRGPVRLLRE
                     LGTAEALRFAHLLLLPAGVMAEKLFTGESARLLLLGNAMHADVPIDAPGSAVMGYMLI
                     MMAQDGGFPVPVGGAGKLAEALVNRARSAGAHIECGREVEAIEVHGGRAVAVHTTDGS
                     TVKVRRAVVADTSAPRLFCDLLPSHAVPNGLKRSLDHFVWDTPVLKVNYALDSAIPWQ
                     SKSLNDAGTVHLGADHDGLVRWMADLNTKTVPRWPFMLFGQMTTADPTRSPAGTESAW
                     AYTHLPRGVADDESADR"
BASE COUNT          316 a          592 c          644 g          314 t
ORIGIN      
        1 cggctccgcc ggggcggatg tcgtcccggt gcaggccgac ctgcgtcggc cggaggaggt
       61 gcagcggctg taccgcaccg caacggaagg cggccgtcat ctgacggcag cggcgctcaa
      121 tgcgggcacc ggcggagcgg gcaaattcgt ggagcgagat ctcgagagcg acctcgacgt
      181 cgtggacctc aacgtccgat ccacggtgct tctttccaaa ctcgttctgc aggacatggc
      241 cgcacagggg tccggcaagg tgctgtatac gtcatccatc gtcgcccaga tgccgggttc
      301 ccatcaggcg atgtacgcgg catccaagtc gttcgtgcag tccttcgccg aagcccttca
      361 tgacgagatg cgcgacaccg gcgtgaccgt cacgtcattg atgccgggcc cgaccgacac
      421 caatttcttc gcgcgcctcg gtatggccga caccgtcgta ggccgcatgc cgaaggacga
      481 tcctggcgag gtcgccaagc agggctatga cgcgatgatg cgcggcgagc agaaggtcgt
      541 cgcggcgtct ccgatgtcca aggcgatggg actggtcaat cggttcctgc cggactcggt
      601 caaagccgtt gcccaccgat tgatttcgca gccgccgcca tgattcgaca aaccgccgat
      661 gccgtggtga tcggcgccgg acacaacggc ctggtggccg cggcgatgct cgccgatgcc
      721 gggtgggatg tgttggtgct cgaggcacag cccgagcccg gcggcgcggt gaagagcgct
      781 gagttgtttc ccggcttcgt cagcgatctg tacagcgcgt tctatccgct gtcggtggtc
      841 tcgccggcgc tgcgggcact gaacctcgaa gaccacggct tgcagtggac gcatgcgccc
      901 ggggtggtcg gtcatgcgcg atcggccgac gacgatgacg caccggtgat ctaccgcgac
      961 atcgaccgga cgatcgccga cctgaatcga cgcgatccag gcgacggcga ccagtggggc
     1021 gaactgttcg aacagtggca gcggatcagc cagccgctgc tgtcgacgct gttctcgccg
     1081 tttccgccgt tgcggggacc cgtccggctg ctgcgcgagc tgggcaccgc cgaggcgctg
     1141 aggttcgccc acctgctgct gctgcccgca ggagtgatgg cggagaagct gttcaccggc
     1201 gaatcggcgc gattgttgtt gttgggcaac gcgatgcatg cggacgtgcc catcgacgcg
     1261 cccggcagcg cagtgatggg ttacatgctc atcatgatgg cccaggacgg cggcttcccg
     1321 gtacctgtgg gcggtgcggg caagttagcc gaggcactgg tcaaccgggc gcgatcagcc
     1381 ggcgcgcaca tcgaatgcgg acgtgaggtc gaggccatcg aggtgcacgg cggacgcgcg
     1441 gtcgccgtgc acaccaccga cggctcgacg gtgaaggtgc gccgagccgt ggtggccgac
     1501 acgtcggcgc cgaggctgtt ctgcgacctg ctgccatcac atgccgtgcc gaacggactg
     1561 aagagatcgc tggaccattt cgtctgggac acaccggttc tcaaagtcaa ctatgcgctg
     1621 gattcggcga taccgtggca gtccaagagc ttgaacgatg cgggcaccgt gcacctcggc
     1681 gccgaccacg acggcctcgt acggtggatg gcggacctga acacaaagac ggttccgcga
     1741 tggccgttca tgctgttcgg ccagatgacc acggcggacc cgacccgctc accggcggga
     1801 accgaaagcg cttgggctta cacccatctg ccgcgcggtg tggcagacga tgagtccgcc
     1861 gatcgg
//
LOCUS       DASYLX010000966         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2474328,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000966 DASYLX010000000
VERSION     DASYLX010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474328"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VGP27_22530"
     CDS             complement(<1..789)
                     /locus_tag="VGP27_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854106.1"
                     /translation="MTGPGPLAGIRILEVGVMLAGPYATMLLADLGAEVIKIEPPDGE
                     ISRQVSDSYFASLNRNKQSICVDLRSEAGQTELGELVANSHALLVNMKPSAIRRLGLT
                     YEALRRFNEKIVCVALTGFGMDGGDDPAFDYVIQAGTGVAAMTGNPDGPPTLPGYSAA
                     DNSTGLTAALGLLAQIVSGRGGQVEVSLRDVVMSQLNYRASAYLNDGIEPQRYPNGAH
                     SYYVPAQLFPTADGYLALFITHDGFWRSFSAEAGISGFPTMAERA"
     gene            complement(786..>1629)
                     /locus_tag="VGP27_22535"
     CDS             complement(786..>1629)
                     /locus_tag="VGP27_22535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854107.1"
                     /translation="PQVPDDFDALTMVALMAAHTSRIELGTAVVPLQAQHPIALARQA
                     LSVHATASGRLALGVGPSHHWIVRDMLGIPYDKPAAYTRDYLEVLNAAIGGPGPVDVE
                     NDSFTVHNPTVLAAETPMPVLVAALGPVMLQIAGEHADGTVLWMADEKAIGDHIAPKI
                     TKAAADAGRPAPRIVAGIPVCLCANSEIDAAKERANRILAEAETSPNYQRLLDRGEAR
                     DVGDLCAAGDQDAILARFRQFADAGVTDLSVRLLPIGDNREELIASKYRTREVIADLA
                     KAVR"
BASE COUNT          272 a          559 c          533 g          265 t
ORIGIN      
        1 agcccgctcg gccatggtcg ggaaccccga tatgccggct tcggccgaga acgatcgcca
       61 gaaaccgtcg tgggtgatga acagcgcgag atagccatcg gccgtcggaa acaattgagc
      121 aggaacataa tacgagtgcg caccgttcgg gtatcgctgc ggctcgatcc cgtcgttcaa
      181 atacgccgag gcgcggtagt tcagctgcga catcaccacg tcgcgcagcg acacctcgac
      241 ctgcccgccg cggcccgaca cgatctgggc caacaggcca agcgcggcgg tcagcccggt
      301 ggagttgtcg gccgccgaat agccgggcag tgtcggcggg ccgtcgggat tccccgtcat
      361 cgccgccacc ccggtccccg cctggatcac gtagtcgaac gccggatcgt cgccgccgtc
      421 cattccgaaa ccggtcagcg cgacacatac gatcttctcg ttgaagcgcc gcagcgcctc
      481 ataggtaagg ccgagccgcc gaatcgccga cggcttcatg ttgaccagca gcgcatggga
      541 attcgcgaca agctcgccga gttccgtctg cccggcctcc gaccgcagat ccacacagat
      601 gctctgcttg ttgcggttca ggctggcgaa atagctgtcg ctgacctgac gggagatctc
      661 gccgtcgggc ggttcgatct tgatcacctc ggcgccgagg tcggcgagca gcatcgtcgc
      721 gtacgggcct gccagcatga cgccgacctc gagaattcgg atgcccgcca gcggtccggg
      781 gccggtcacc gtaccgcctt ggcgaggtcg gcgatcacct cgcgggtgcg gtatttcgac
      841 gcgatcagct cttcgcggtt gtcaccgatc gggagcagcc gcaccgacag atcggtgacg
      901 cccgcatcgg cgaactgacg gaaccgcgcc aggatagcgt cctgatcgcc cgcggcgcac
      961 agatctccga cgtcccgcgc ttcgccgcga tccagcagcc gttgatagtt cggcgacgtc
     1021 tccgcctcgg ccagaatgcg attcgcgcgt tccttggccg catcgatctc cgagttggcg
     1081 cacagacaca ccggaatacc cgcgacgatg cgcggcgccg gccgccccgc gtcggcggcg
     1141 gccttggtga tcttcggtgc gatgtggtcg ccgatggcct tctcgtcggc catccacagc
     1201 actgtgccgt cggcgtgctc gcccgcgatc tgcagcatca ccggcccgag cgccgcgacc
     1261 agcaccggca tcggcgtctc ggcagccagc accgtcgggt tgtgcacagt gaacgagtcg
     1321 ttctcgacgt ccaccggacc cggtccgccg atcgcggcgt tgagcacctc gagatagtcg
     1381 cgggtgtagg ccgccggctt gtcgtagggg atgccgagca tgtcccgcac gatccagtgg
     1441 tgcgaaggac cgacgccgag agccaaccgt ccggatgccg tcgcgtggac cgaaagcgct
     1501 tgacgagcaa gggcgatcgg atgttgagcc tgcagcggca ccaccgcggt gccgagttcg
     1561 atgcgcgagg tgtgtgcggc catcagcgcc accatggtca gcgcgtcgaa gtcgtcgggc
     1621 acctgcggc
//
LOCUS       DASYLX010000967         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2583916,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000967 DASYLX010000000
VERSION     DASYLX010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583916"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VGP27_22540"
     CDS             complement(<1..298)
                     /locus_tag="VGP27_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEV7854108.1"
                     /translation="MAGSAGRSEATRGRGRIPDRDIAAIRERVQIEDVVGDYVQLRRA
                     GADSLKGLCPFHDERSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKMEHVSFVEAV"
     gene            complement(303..>1555)
                     /locus_tag="VGP27_22545"
     CDS             complement(303..>1555)
                     /locus_tag="VGP27_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEV7854109.1"
                     /translation="NDAYDEFDRQRVVVEPAKSAALPGTDTEHRTDFARDRARVLHCA
                     ALRRLADKTQVVGPRQSDTPRTRLTHSLEVAQIGRGMALGLGCDPDLVDMAGLAHDIG
                     HPPYGHNGERALNEIADAHGGFEGNAQNFRILTRLEPKVLDAHDRSAGLNLTRAALDA
                     VTKYPWQRSPQRSKFGFYADDTAAADWMRQRAPQFRPCLEAQVMDWADDVAYSVHDVE
                     DGVVSGRIDMRVLADDDAAAELGRLGESTGMGGGLSAEDLVSAAGRLSELPMVAAVGK
                     YDATLAASVALKKLTSELVGRFASAAIAATWAEAGPGPLVRYATDLRVPALVRAEVTL
                     LKVLALQFIMSDPRHLDLQGRQRDLIHRVAEWLSAGAPATLDPVFVPAFNAAADDGER
                     QRVVVDQIASFTEGRLERLEPALD"
BASE COUNT          248 a          569 c          495 g          243 t
ORIGIN      
        1 cgaccgcctc gacgaagctg acgtgctcca tcttctgcac gaacgcatac acgtcgccgc
       61 cctcgccaca gccgaagcag tggaagtggc cgtggttcgg ccgcacatga aacgacgggg
      121 atctctcgtc gtggaacggg cacagaccct tcagcgaatc agccccggcg cggcgcaact
      181 ggacatagtc gccgaccacg tcttcgattt gcacacgttc acgaatggcc gcgatatcgc
      241 gatcagggat ccttccccga ccccgagtcg cttcgctcct acccgccgaa ccggccaccg
      301 aatcagtcta gagcgggttc caaccgctcc aatcggcctt cggtgaacga cgctatctgg
      361 tcgacgacca cccgctgccg ctcaccgtcg tcggccgccg cgttgaacgc gggcacgaag
      421 accgggtcga gcgtggcggg cgcaccggcc gacaaccact ccgcgactcg gtggatcagg
      481 tcacgctggc ggccctgcag gtccaggtgc cgcggatcgg acatgatgaa ctgcagcgcc
      541 agcaccttga gcagcgtcac ctcggcgcgc acgagagccg gtacccgcag atcggtggca
      601 tagcggacca gggggccagg ccctgcctcg gcccaggtgg cggcaatcgc ggccgacgcg
      661 aaccgcccga ccagctcact ggtcagcttc ttcagcgcga ccgacgcggc caacgtggcg
      721 tcgtacttgc cgacggcggc aaccatcggc aactcggaca gccgccccgc cgccgacacc
      781 aggtcctcgg cggacagccc cccgcccatg ccggtcgact cccccagtcg acccagctcg
      841 gccgcggcgt cgtcgtcggc gagcacgcgc atgtcgatcc ggcccgacac cactccgtcc
      901 tcgacgtcgt gcaccgagta cgccacgtcg tcggcccaat ccatcacctg agcctccagg
      961 cacgggcgga actgcggcgc ccgctgccgc atccagtcgg cggcggccgt gtcgtcggcg
     1021 tagaagccga acttgctccg ctgcgggctg cgctgccacg gatatttcgt gacggcgtcc
     1081 agcgccgccc gcgtcaagtt caaacctgcg ctgcggtcat gtgcgtcaag aactttgggc
     1141 tccaggcgag tcaggatcct gaagttctgc gcattgcctt cgaagccacc atgtgcgtcg
     1201 gcgatctcgt tcagggcgcg ctcgccgttg tgcccgtacg gcggatgccc gatgtcatgg
     1261 gcgagtcccg ccatgtcgac gagatcgggg tcacacccca gcccgagggc catgccgcgg
     1321 ccgatctggg ccacctcgag ggagtgcgtc agccgggtgc gcggagtgtc gctctggcgg
     1381 ggtccgacca cctgggtctt gtcggccagt cgacgcagcg ccgcacagtg cagtacccgc
     1441 gcccggtcgc gggcgaagtc ggtgcggtgt tcggtgtccg tccccggtaa cgcggcactc
     1501 ttcgccggct cgaccaccac acgttgccgg tcgaactcgt cgtatgcgtc gttgt
//
LOCUS       DASYLX010000968         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1598615,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000968 DASYLX010000000
VERSION     DASYLX010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598615"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VGP27_22550"
     CDS             complement(<1..1161)
                     /locus_tag="VGP27_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HEV7854110.1"
                     /translation="MSTNRFNAVVRWPRALAYAMAAVVMGVVVGELAAMLIFAGSSDR
                     RLDEQAARAADSAPAVVAASGDLEKTRQARTTLDDAVVDAGRRRDEALVVARCEFNPS
                     PACPQNHITGQPGAGPENRTANGFLANTQQQLDRAVAERDRLAPSLDAEAAAGQQKLT
                     TSRDAAMATADRGFGARWLAMNAYTLDSPGATVLRALLAGLFIVLFLFPLILKLSRAK
                     TTEDRRTLARAQQERANLEADTAIAVKRAEVRAAIETMRAEQELASTRLAIEAQNEID
                     RAQQRRRVAAAIEAPTPVTSERVVEPVAELTAEPVLEESDNLPALTESADAGEKRSLI
                     PTVPGVAGAAVRWMRPFVPPIVASAIDTTTRPLRAARQVFEETEEIHVSIRRS"
     gene            1327..>1518
                     /locus_tag="VGP27_22555"
     CDS             1327..>1518
                     /locus_tag="VGP27_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854111.1"
                     /translation="MGSRLISLTAVIAAMTVTAGCGLTGTEPTEPSPGRVTVGDESRQ
                     TQSVACTQNDWDLTIVARTD"
BASE COUNT          237 a          543 c          492 g          246 t
ORIGIN      
        1 gcttcgcctg atgctgacgt ggatctcctc tgtctcctcg aacacctggc gcgcggcccg
       61 caatggcctc gtcgtcgtat cgatggcgct ggcgacgatc ggcggcacga acggacgcat
      121 ccaccgcacc gccgcccccg cgacaccggg aaccgtcggg atcaacgagc gcttctcgcc
      181 cgcatccgcc gactcggtca acgcgggcag gttgtccgac tcttcgagca ctggttcggc
      241 cgtcaattcg gcgacgggct cgacgacccg ctccgaggtc accggcgtcg gcgcctcgat
      301 cgccgcggcg acgcgacggc gctgctgtgc ccgatcgatc tcgttctgcg cctcgatcgc
      361 caggcgcgta ctggccagct cctgctcggc gcgcatcgtc tcaatcgcgg cgcgcacctc
      421 ggcccgcttc accgcgatcg ccgtatccgc ctccagattc gcccgttcct gctgcgcgcg
      481 cgccaacgtg cgccgatcct cggtcgtctt ggcccgcgac agcttgagga tcagcgggaa
      541 gaggaacagc acgatgaaca agccggccag caacgcgcgc aacaccgtcg ctccggggct
      601 gtcgagcgtg tacgcgttca tcgccagcca ccgcgcgccg aacccacggt cggcagtcgc
      661 catcgcggca tcgcgagacg tggtgagctt ctgctgcccg gcggcagcct cggcgtccaa
      721 agacggggcg agccggtcgc gctcggccac cgccctgtcc agctgctgtt gggtgttcgc
      781 gagaaaccca ttggccgtgc ggttttcggg cccggcgccg ggctgtccgg tgatgtggtt
      841 ctgcgggcag gccggcgacg ggttgaattc gcagcgcgcg acgaccaacg cttcgtcgcg
      901 tcggcggccc gcgtccacca cagcgtcatc gagagtcgtg cgtgcctgac gcgtcttctc
      961 gaggtctccc gacgcggcca cgacggcggg tgcggaatcg gcggcgcgcg ccgcctgctc
     1021 gtcgagtcgg cgatcgctcg aacccgcgaa gatcagcatc gcggccagct cgccgacgac
     1081 gacacccatg acgaccgcgg ccatcgcata cgccaatgcg cgcggccatc tgacaacagc
     1141 attgaaacga ttggtagaca tcggaactcc ataaactgga ttacgttgga cgcggcggaa
     1201 ttctcacgcc ggcgtgggtt gaccagatct tctcataccc gccgtcgctc ccgcacctcc
     1261 tggcggtgct gctcgacgtg tagccgacac cacatagtgg gtaaccccgc cgcaagggag
     1321 gtgctcgtgg ggagtcggtt gatcagcctg acagcagtga ttgcggcaat gaccgtgacc
     1381 gccgggtgtg ggctgacggg cacagaaccg accgaaccgt ctcccggccg cgtcaccgtc
     1441 ggcgacgagt cgcggcagac gcaatccgtc gcctgcactc agaacgattg ggacttgacg
     1501 atcgtcgcgc gcaccgac
//
LOCUS       DASYLX010000969         9578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_610085,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000969 DASYLX010000000
VERSION     DASYLX010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610085"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..819)
                     /locus_tag="VGP27_22560"
     CDS             complement(169..819)
                     /locus_tag="VGP27_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEV7854112.1"
                     /translation="MLTELIPLALVVALSPLSIIPAVLVLHTPRPRPTGLAFLAGWLL
                     GLTALTAIFLELSSLAGGMRDKPPTWASWVRIIVGAALIVFGLYRWFARKRSAHTPGW
                     MNSMSKFTPPRAILAAAALTVVNVKVLFMCAAAGLAIGSAGLRAPGAWVAVAWFVLVA
                     GSTVAIPVLAYAVAGDRLDQPLARLKDWMERQHATLVAVILVVIGLLVLYKGIHGL"
     gene            962..1456
                     /locus_tag="VGP27_22565"
     CDS             962..1456
                     /locus_tag="VGP27_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854113.1"
                     /translation="MTRFTIAGCAAVVTLVAVPAAVATVHPQATARADSCSDAASNMP
                     LAMDSGPCADVLTQERRWLTAITEGDRATVESILAPSFKHINAEGKLLDRAQEVTGVE
                     KVSFTMNPSEQLVDIAGDTAVIHGLNTLIDNGKVLARERFTDVFQLQNGTWIALSAQE
                     TTLG"
     gene            1545..4727
                     /locus_tag="VGP27_22570"
     CDS             1545..4727
                     /locus_tag="VGP27_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV7854114.1"
                     /translation="MRRLRTKSSIVIALTVVLSMTAAMTACSKPNTAPVPQAGVNASP
                     DGRPAPFKEPVRLSSKDGVLEVRLSAHQSTVNLDTVKEPVTNFLTYGFDVIKGTSSDG
                     SAKGDNLYPGPTLRVEPGEKLIVHYDNDLAGLTIQDYYDPAFTPKGGEVPVYPPTLKE
                     SPLNLHTHGLHVSPSGNADNVLLSIPAGEGNTYTYDVPKTMPNGLYWYHSHRHTMTSQ
                     QTYFGLAGLLEVGRPDGNLPLVTKNNIPVRDMAIQYNFVFDRNGKGHQLNNYSWPQFV
                     STFKPPEGDQLGDGTYQPSLAPVNMADTTEGAKYITPWWSGPLSPKNNRGQTQFMPSN
                     LIGFDSPTEKVAENPALPENQRDVQFTVNGQFQPELKIKPGQTEIWVVANISDIAYMT
                     VRLTETATGNHPKFSIVGQDGNPYTQVGRPVYGDGSTLDIPPASRYAIAVTMPKDGDL
                     VLEFPPDPHAREIVDPGVLYTNNGTKNPPAVLGTLSVDPKYISYADGFFVFPTQTLIR
                     ATPDTSGQGQTTAFEPGQSLDAYTSFVDTSVMTPAVKRALTINDNIGGDKASNNDPKA
                     VIYQFDDNAFPNIPLIQPRLNSVEEWLITNFNNDAHPMHIHVNDFQVTQIDDPNRGRT
                     GVQPWGLDNVNVPAPIFDDQHVVSTPASLTLRQEFLEFVGTYVIHCHRLNHEDNGLMA
                     LINVIPEVSTYAVAIPGSNGKPASVQVRDANGDRVLQTVYPFPDFEGTPSVAMADVNG
                     DGILDLVAGTGRGASPEVVAYDGNNTVDGVFKTEIARFAPFDASFTGGVTVAGADIDG
                     NAMADNIIVGSGPGMESQVKVFASKLPGESGKAPDVFSTFTPYPGSQSGVALATGMVE
                     AGSGRETVVTAPGPGDAPLVKSFRWDLFTPTARAQASGTATQGHLGKPNDPKMTSQFM
                     AYDENYKGGIGLSTGWVAGAEGGAKSIVTSQLAGEGTVKVWSNGSRLDGNPPIYLDNP
                     NHHDEDVKYAEIASFVPFPGAKPGVTVATSSTAYGADLLVAGITPGGQEVRKYTLERP
                     APDAKTVAPKQIANLPVIPGVAAAAPLAGR"
     gene            complement(4724..5695)
                     /locus_tag="VGP27_22575"
     CDS             complement(4724..5695)
                     /locus_tag="VGP27_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV7854115.1"
                     /translation="MDIRNPTATAATIIIGQKVRAGSELRFEEWQKDTNREASKYPGF
                     IGAEINPPTAVQPEWVVVYRFDSVPHVMAWLNSAARQERLTAGQEFFDGPGTQQILGG
                     GARTADPLVTVAVSHRVTPEDVEDFLAWQERLRLAESQFPGFRGTELFRPVAGLQEEW
                     TALYRYDSAADLDRWLVSDERKQLLAEGERFADFKSRTIDNSFGSWFAFDDHGNEAPP
                     PSDIKTSVAVWVGLYPTVVFLSLLFWPLKMPLWLNLLVGNLCSSFAMTFVTMPYYVNP
                     LLRRWLRPPPNVPAAKNNRRGITIAVCAMTFWTVVIYLVTRVFWHLP"
     gene            complement(5695..8439)
                     /locus_tag="VGP27_22580"
     CDS             complement(5695..8439)
                     /locus_tag="VGP27_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7854116.1"
                     /translation="MTYTVNGKAFDEEPGPGQCLRTFVRSLGWHGVKKGCDAGDCGAC
                     TVWLDGDPVHSCITPAFRAEGREVTTIEGLGTPEALHPLQRQFRDAPGFQCGFCTAGM
                     IMTSATFTDEQRQNLPRALKGNLCRCTGYRAIEDAINGVVEIEQAAPGQAVGASVGAP
                     AGTDVVTGRAEYTMDTEMDGMLHLKVLHSPHAHARIVSIDTTAALVVPGVHRVYTWQD
                     VPQKRFNTAIHTDHLVDPDDTLILDNVVRFVGQRVVAVVADSVGAAEEGCRRVEVAYD
                     VLPAVFDPEEAMTDGAPQLHGTHDPFIHDPAHNILIELHSEIGDAAAGFAEADVIHEG
                     TYFSPRVQHAHLETHGSIAWMEDGRLNVRTSSQSPSICKLKLSHLFDLRPDQLRVFCK
                     RVGGGFGGKQEVISEDLVSLATIDIGRPVCWEYTREEEFTTASPRHPMKITVRLGAKS
                     DGTLTAFHFRNVSNTGAYGNHGGETLFAGGAAVMQYRCPNKKFDGYSVYTNTVPGGAL
                     RGYGMTQPTFAVESAMTELALALNLDPMELRRRNVIRPGDALLAIGEHPHDVTFTEDG
                     LTACIDMVDAALQRSANGHALGEDWLIGTGTASSIHETAPPTEHISDAWASLRDDGIY
                     EIAVGTVEFGEGTSTAHVQIAASVLGTTPSRVHLVQSDTDRTGFDTGAFASAGLFVSG
                     NAVQKASTALRDGVLRFAANHTGVEVASCTLLHDAVQCGDVTLPMAELLAAARERRIR
                     FSVSRKAYGSPRSVTSNAHGFRIAVHRITGEIRILYSVHAADAGVVINPAQVRGQIEG
                     GVAQAIGFALTENFHVDRNGVMTNPNLRNYRIPTFADIPRTEVIVVETGDSVGPMGAK
                     GIAESNVNPVAPALANALQDATGVRYRELPFTPERIYRRLVDNALAVSASHVREIPAN
                     "
     gene            complement(8436..9242)
                     /locus_tag="VGP27_22585"
     CDS             complement(8436..9242)
                     /locus_tag="VGP27_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7854117.1"
                     /translation="MDINTVTEVVRRPSGQPGSDWRDGDAWLAGGTWLFSDQQPDVRR
                     LIDLVPLGWDGLTATDAGLEIGAMCTIRRLYEFPAPSDWPAAPLLRTSCEAFLASFKV
                     WNAATVGGNIAMSLPAGPMITMTVALQATYTLQSTDGSERIVDAADFVTGDHENVLRP
                     GELLRKIDIPAGALRKRHAHRRFTLTKLGRSTIFIVATQGAGDLLLTITAGTTRPVRF
                     AFDSVPDADTLAHSIDSIPEDVWFDDPNGTPDHRRHLARHFAEEIRGELA"
BASE COUNT         1753 a         3129 c         3106 g         1590 t
ORIGIN      
        1 cgaaccattt tgtgtagaac tggcggatca gttcttcgcc cccgaggcgt gggtcgaccg
       61 cgtcgcgcag catccgcagc ccgtcacggt catcgagtcc cacgccgctc gagtctaggt
      121 gagtcgagcg gatgcgcggc gcggagcacc aggccggagc tgcccgggct atagcccgtg
      181 gatgcccttg tacagcacca gaagaccgat gacgacgagg atcacagcga ccaatgtcgc
      241 gtgctgacgt tccatccagt ctttgagccg agcgaggggt tggtcgagac ggtcgcccgc
      301 caccgcgtag gcgaggacgg gaatcgcgac agtcgacccg gctaccagga cgaaccacgc
      361 cacggcgacc cacgcgcccg gtgctcgcag tcctgcggaa ccgatcgcca gacccgctgc
      421 ggcacacatg aacaggacct tcacgttgac caccgtcaac gccgcggcgg cgaggatcgc
      481 gcgtggcgga gtgaacttgc tcatgctgtt catccagcca ggggtgtgcg ccgagcgctt
      541 gcgggcgaac cagcggtaga gaccgaagac gatcagcgcc gcaccgacga tgatgcgcac
      601 ccaagaagcc caggtcggcg gcttgtcacg catgccgccg gcaaggctgg acagctcgag
      661 gaagatggcc gtcaacgccg tgagcccgag cagccagccc gcgagaaacg ccagtcccgt
      721 cggcctcggc cgcggagtgt gcagcaccag aaccgccgga atgatcgaca acggcgacag
      781 cgcgacgacc agcgccagcg ggatgagctc ggtcagcacg gtgagaaggt agcaagccgg
      841 gcagccgaaa tggaggcgaa atgtcggttt acgatttgcc gctggccggc ttccacgccg
      901 agggagattc cgctaccgat ttgccgtcgg acgtcctgcg cggctgacat actcgcgttc
      961 catgacgcga ttcactattg ccgggtgcgc ggcggtggtc acgctcgtgg ccgttcctgc
     1021 cgccgtcgca accgtgcacc cgcaggctac cgctcgagca gacagctgct ccgacgccgc
     1081 ctccaacatg ccgctggcga tggattccgg tccctgcgca gacgtgctca cgcaggagag
     1141 gcggtggttg acggcgatca ccgagggcga ccgggcgacc gtggaatcga ttctggcacc
     1201 gagcttcaag cacatcaacg ccgaaggaaa gctgctcgac cgggcacagg aagtgactgg
     1261 agtagagaaa gtctcgttca cgatgaaccc gtcggagcag ctcgtcgaca tcgcgggcga
     1321 caccgcggtc attcacggtc tgaacaccct catcgacaac ggcaaggtgc tggcgcgcga
     1381 gcgcttcacc gacgtattcc agctgcagaa cggcacctgg atcgcactgt ccgcccagga
     1441 gacgacgctc ggataattcg ttgcggctga tttgccgcgg atgtaagccc agctatgtag
     1501 tgtttttgct gcattcccac ccgcgtcctg aaatggggag cgctatgcgg cgtcttcgca
     1561 ccaagtcctc aatcgtcatc gcgctgactg tcgtcctatc gatgaccgcg gcgatgacgg
     1621 cctgcagcaa acccaacacg gcacccgtcc cgcaggccgg cgtcaacgcc agtccggacg
     1681 gcaggcctgc gcccttcaag gagccggtcc ggctctccag caaggacggc gttctcgagg
     1741 tccggttatc ggcgcaccag tcgaccgtca acctcgatac ggtcaaagag cccgtcacca
     1801 acttcctcac ctacggcttc gacgtgatca agggcaccag ttcggacggc tcggccaaag
     1861 gcgacaacct ctacccgggc ccgacgctgc gcgtcgagcc cggcgagaag ctgatcgtgc
     1921 actatgacaa cgaccttgcg ggtctgacca tccaggacta ctacgacccc gccttcacgc
     1981 cgaagggtgg cgaggtaccc gtctacccgc cgacgctcaa ggagtcgccc ctgaacctgc
     2041 acacccacgg cctgcacgtc agcccctcgg gcaatgccga caacgtgctg ttgtcgattc
     2101 cggcgggcga gggcaacacc tacacctacg acgtgcccaa gacaatgccc aacggtctgt
     2161 actggtatca cagccaccgc cacacgatga cttcccagca gacctatttc ggcctggccg
     2221 gcctgctcga ggttgggcgc cccgatggga atctgccgct ggtgaccaag aacaacattc
     2281 cggtgcgcga catggccatt caatacaact tcgttttcga caggaacggt aagggtcatc
     2341 agctgaacaa ctacagctgg ccgcagttcg tcagcacgtt caagccgcct gagggcgacc
     2401 aactaggcga tgggacatat cagccaagtc tggctccggt gaacatggcc gacaccaccg
     2461 aaggggcgaa gtacatcacg ccatggtggt cgggcccgct gtcgccgaag aacaatcgcg
     2521 gtcagaccca gttcatgccg tcgaacctga tcggcttcga cagcccgacc gagaaggtcg
     2581 ccgagaaccc ggcgctgccg gagaaccagc gcgacgtgca gttcaccgtc aatggccagt
     2641 tccagccgga gctgaagatc aagccgggcc agacggagat ctgggtggtt gccaacatca
     2701 gcgacatcgc ctacatgaca gtcaggttga ccgagaccgc aacggggaat cacccgaagt
     2761 tctcgatcgt cggtcaagac ggcaacccct atacgcaggt cggcaggccc gtctacggcg
     2821 acggcagcac cctcgacatc cctccggcgt cacggtacgc aatcgcggtc accatgccca
     2881 aggacggcga tctggtactc gagttccctc cggatcccca tgccagggaa atcgtggatc
     2941 ccggtgtgct ctataccaac aacggcacca agaacccgcc tgccgtactc ggcacgctgt
     3001 cggtggaccc gaagtacatc agctacgccg acgggttctt cgtcttcccg acccagaccc
     3061 ttatccgcgc gacaccggac accagcggcc aagggcagac cacggccttc gagccggggc
     3121 agagcctcga cgcgtacacg tcgttcgtgg acacctcggt gatgacgccc gccgtgaaac
     3181 gcgcgttgac catcaacgac aacatcggtg gtgacaaggc cagcaacaac gaccccaagg
     3241 cagtcatcta ccagttcgac gacaacgcct tcccgaacat cccgctgatc cagccgcgac
     3301 tgaactcggt cgaggagtgg ctgatcacca acttcaacaa cgatgcccac ccgatgcaca
     3361 tccacgtcaa cgacttccag gtgacgcaga tcgacgaccc gaaccggggc aggacgggcg
     3421 ttcagccgtg gggattggac aacgtcaacg tgcccgcacc aattttcgac gaccaacatg
     3481 tcgtcagcac gcctgcgtcg ctcaccctgc ggcaggagtt cctcgagttc gtcggcacct
     3541 acgtcattca ctgccaccgg ctcaaccacg aggacaacgg gctgatggcg ctgatcaacg
     3601 tgatccccga ggtgtcgacg tacgcggtgg cgatacccgg gtccaatggc aagccggcct
     3661 cggttcaggt ccgggatgcg aacggggaca gggtgctaca aaccgtgtac ccgttccccg
     3721 acttcgaggg cacgccgtcg gtggcgatgg ccgacgtgaa cggcgacgga atcctcgatc
     3781 tcgtcgccgg cacgggtagg ggtgcatctc ccgaggtggt cgcctacgac ggcaacaaca
     3841 ccgtggacgg agtcttcaag accgagatcg cccgcttcgc gcccttcgac gccagcttca
     3901 ccggcggggt gaccgtcgcg ggcgccgaca tcgacggaaa cgcgatggcc gacaacatca
     3961 tcgtcggctc cggtcctggg atggaatcgc aggtgaaggt gttcgcctcg aagctgcccg
     4021 gcgaatcggg caaggcgccc gacgtgttct ccacgttcac gccgtacccc ggttcgcagt
     4081 ccggtgtcgc gctcgccacc ggcatggtcg aggcgggttc gggccgggaa accgtggtga
     4141 ccgcgccggg tcccggtgac gcgccgctgg tcaaatcctt ccgatgggat cttttcaccc
     4201 cgacggcgcg ggcccaggcc agtggcactg cgacacaggg gcatttgggc aagccgaacg
     4261 atccgaagat gacgtcgcag ttcatggcgt acgacgagaa ctacaagggc ggcatcgggt
     4321 tgtcgaccgg gtgggtcgcg ggtgccgagg gcggcgcgaa gagcatcgtc accagtcagc
     4381 tcgcgggtga gggcacggtc aaggtgtggt ccaacgggtc gcgactcgac gggaacccgc
     4441 ccatctacct cgacaacccg aatcatcatg acgaggacgt caagtacgcc gagatcgcgt
     4501 cgttcgtccc gttccccggt gccaagcctg gcgtgaccgt ggcgacgagc agcactgcct
     4561 atggcgctga cctgttggtg gcgggcatca cgcccggcgg tcaggaggtc cgcaagtaca
     4621 ccctggagcg cccggcgccg gacgcgaaaa ctgttgctcc taagcagatt gcgaaccttc
     4681 cggtgatacc gggtgtcgcc gccgccgcac cgcttgccgg ccgctagggc agatgccaga
     4741 agactctggt caccaagtag atgacgacgg tccagaacgt catcgcacag accgcgatgg
     4801 tgattcctcg tcggttgttc ttggctgcgg gcacatttgg gggcggtcgg agccagcgcc
     4861 tcaatagcgg gttgacgtag tacggcatcg tcacgaaggt catggcgaag ctggaacaca
     4921 agttgccgac gagcaggttc agccatagcg gcatcttcaa cggccagaac aaaagcgaca
     4981 ggaacaccac cgtcgggtag aggcccaccc ataccgcgac ggacgtcttg atgtccgagg
     5041 gcggaggcgc ctcgttgccg tggtcgtcga aagcgaacca actgccgaac gagttgtcga
     5101 tggtgcggga cttgaagtcg gcgaatcgct caccctccgc caacagctgc ttgcgttcgt
     5161 cggagaccag ccacctgtcc aggtcggcgg cgctgtcgta gcggtagagg gcggtccact
     5221 cctcctgcag cccggcgacc ggccggaaca actccgtgcc gcggaagccg gggaactggc
     5281 tttccgccag gcgaagtcgt tcctgccatg cgaggaagtc ctcgacgtcc tccggagtca
     5341 cgcggtggct gaccgccacc gtcaccagcg ggtctgcggt acgtgcaccg cctccgagaa
     5401 tctgctgggt gcccgggccg tcgaagaact cctggcctgc ggtgaggcgc tcctgacgcg
     5461 cggcgctgtt gagccaggcc atcacatgag gcacggagtc gaagcggtac accaccaccc
     5521 actccggctg taccgcggtc ggcggattga tctcggcgcc aatgaaaccc ggatacttcg
     5581 acgcctcgcg gttggtgtcc ttctgccact cctcgaagcg cagttccgat ccggctcgca
     5641 ccttctgccc gatgatgatg gtggcagcgg tcgcggtggg attcctgatg tccatcagtt
     5701 cgctgggatt tcgcgcacgt gagaggcaga gactgccaga gcgttgtcga cgagcctgcg
     5761 gtagatccgc tcgggcgtga acggcaattc gcggtagcga acgcctgtgg cgtcctgcag
     5821 cgcgttggcc agcgccgggg ccaccgggtt gacgttcgac tcggcaattc ccttggcgcc
     5881 catcggaccg acggagtcgc ccgtctcgac gacgatgact tcggtgcggg gaatgtcggc
     5941 gaaggtcggg atacggtagt tgcggaggtt ggggttggtc atcaccccgt tgcggtcgac
     6001 gtggaagttc tcggtcagcg cgaagccgat tgcctgggcc accccgccct cgatctggcc
     6061 ccgcacctgc gccgggttga tcacgacgcc agcgtcggca gcatgaacac tgtagaggat
     6121 gcgaatttca ccggtgatcc ggtgcaccgc gatgcggaag ccgtgcgcgt tcgaggtcac
     6181 gctccgcgga gatccgtatg ccttgcggga caccgagaag cggatccgtc gttccctggc
     6241 ggccgccagc aactcggcca tcggcagtgt gacatcgccg cactggacgg cgtcgtgcag
     6301 cagcgtgcag gacgctacct cgacacccgt gtgattggcg gcgaagcgca gaacgccgtc
     6361 tcgcagcgcc gtcgaggcct tctgcacggc gttgccggag acgaacaggc ccgcactggc
     6421 gaaagccccg gtgtcgaagc cggtcctgtc ggtgtcggac tgcacaaggt gcactcgcga
     6481 cggcgtcgtg ccgagcacgc tggcggcgat ctgcacgtgc gccgtcgagg tgccctcgcc
     6541 gaactcgacc gtgccgaccg cgatctcata gatgccgtcg tcgcgcaggg acgcccaagc
     6601 gtcggagatg tgctcggtgg gcggcgcggt ctcgtggatg gaactcgcgg tgccggtccc
     6661 gatcagccag tcttcgccga gcgcgtgacc gttggcgctg cgctgcaacg ccgcgtcgac
     6721 catgtcgatg catgcggtca gcccgtcctc ggtgaacgtg acgtcgtggg gatgctcgcc
     6781 gatggccagc agcgcgtcac cgggacggat cacattgcgc cgcctcagct ccatcggatc
     6841 gaggttcagc gccagcgcca gctcggtcat ggcggattcg acggcgaacg tcggctgcgt
     6901 catcccgtat ccgcgcagcg caccgccggg caccgtgttg gtgtacaccg agtaaccgtc
     6961 gaacttcttg ttgggacaac ggtattgcat caccgcggca ccacccgcga acaacgtctc
     7021 gccgccgtgg ttgccgtacg cgccggtgtt cgacacgttc ctgaagtgga acgctgtcag
     7081 cgtcccatcg gacttggcgc ccagcctgac ggtgatcttc atcgggtgcc tcggcgaggc
     7141 ggtggtgaac tcctcctcgc gggtgtactc ccagcacacc ggccggccga tgtcgatcgt
     7201 cgccagcgaa accaggtcct cggagatcac ctcctgcttg ccgccgaacc cgccgccgac
     7261 ccgcttgcag aacacccgaa gctggtcggg tcgcaggtcg aacaggtgcg acagcttgag
     7321 cttgcagatc gacggcgact gtgagctggt ccgcacgttg agccggccgt cctccatcca
     7381 ggcgatcgag ccgtgcgtct ccaggtgggc atgctggacg cggggagaga agtaggtgcc
     7441 ctcgtgaata acgtcggcct cggcgaaacc cgctgccgca tcgccgatct cactatgcag
     7501 ctcgatgagg atgttgtgcg cgggatcgtg gatgaacggg tcgtgggtgc cgtgcagctg
     7561 tggtgcgccg tccgtcatcg cctcctcggg atcgaagacg gcgggcaaca cgtcgtaggc
     7621 gacctcgact cggcggcagc cctcctcggc ggcgccgacc gagtcggcga ccacggcgac
     7681 gacacgctgg ccgacgaacc gaaccacgtt gtcgaggatc aaggtgtcgt cggggtcgac
     7741 gaggtgatcg gtgtgtatcg ccgtattgaa acgcttctgt gggacgtctt gccaggtgta
     7801 gacgcggtgc acaccgggca ccaccagcgc ggcggtcgtg tcgatcgaca cgatgcgcgc
     7861 atgtgcgtgc ggcgaatgca gcaccttgag gtgcagcatg ccgtccatct cggtgtccat
     7921 cgtgtactcg gccctgccgg tgaccacgtc ggtgcccgcg ggggctccga cactggcgcc
     7981 caccgcttgg cccggcgcgg cctgctcgat ctcgaccacg ccgttgatcg catcctcgat
     8041 tgcccgatat cccgtgcagc ggcacaggtt tcccttcagc gcacgaggca ggttctgcct
     8101 ctgctcgtcg gtgaacgtcg ccgaggtcat gatcatgccc gcggtgcaga aaccgcactg
     8161 gaagccgggg gcgtcgcgga actgtcgttg cagcgggtgc agggcctccg gggtgccgag
     8221 accttcgatc gtggtgacct cgcgtccctc ggcgcggaat gcgggtgtga tgcagctgtg
     8281 caccgggtcg ccgtcgagcc acaccgtgca ggcaccgcaa tcgcctgcgt cgcagccctt
     8341 tttgacgcca tgccagccca gcgagcggac gaatgtgcgg aggcactggc cgggccccgg
     8401 ctcctcgtcg aaagccttgc cgttgacggt gtaggtcatg ccagctctcc cctgatttct
     8461 tcggcgaagt gcctggccag gtgccgccgg tggtccggtg tgccgttcgg gtcgtcgaac
     8521 cacacgtcct cgggtatcga atcgatgctg tgtgcaagcg tgtccgcatc gggtacggag
     8581 tcgaacgcga accgcacagg acgggtggtg cccgccgtga tggtcagcag gaggtccccc
     8641 gccccctgtg tggcaacgat gaagatggtg gaccgcccca gttttgtgag cgtgaagcgc
     8701 ctgtgggcgt ggcgttttcg caaagcaccg gcaggaatgt cgatcttccg cagaagctcg
     8761 ccgggccgca ggacgttctc gtggtcgcca gtcacgaagt ccgctgcgtc gacgatgcgt
     8821 tcggacccgt ccgtcgactg cagcgtgtag gtggcctgga gtgcgacggt catggtgatc
     8881 atcggccccg cgggcagcga catggctatg ttgccgccga ccgtggcggc gttccacacc
     8941 ttgaacgatg cgaggaaggc ctcgcaactg gttcgcaaca acggcgccgc aggccagtcg
     9001 ctcggtgccg gaaattcgta gagcctgcga atcgtgcaca tcgcgccgat ctcgaggccc
     9061 gcgtcggtgg ccgtcagccc gtcccagccg agcggaacca ggtcgatgag gcggcgtacg
     9121 tccggttgct gatcggaaaa cagccaggtc ccgcccgcca accacgcatc gccgtcgcgc
     9181 cagtcgctgc ccggctgtcc ggaaggccgc ctgacgacct cggtgacggt attgatgtcc
     9241 acgtcaatga cccttcgagc gacgggaggc ggaatgtcgg ggccggcgca gccggcgtag
     9301 ctgactctag ccactggcgt cggtgaccgt cggtaattga gaggcgattc aggcatttgc
     9361 caggggcgat gccgcaattg cgcgcgacaa gctaaagttg agcggaacag actcaacctt
     9421 gacggcgttg aggctagcga caagcatttt ctcatccctt tgaggacgga acggaggtag
     9481 tcgtggattc tttcaacccg acgaccaaga cccaggctgc gctgaccgcg gcgctgcagg
     9541 cggcgaccac cgcaggcaac ccgcagatca tgccggcc
//
LOCUS       DASYLX010000970         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3571939,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000970 DASYLX010000000
VERSION     DASYLX010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3571939"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGP27_22590"
     CDS             complement(<1..642)
                     /locus_tag="VGP27_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEV7854118.1"
                     /translation="MNRKNVIRTLTVIAVVLLLGWSFFYFSDNTRGYKPVDTSVAMAQ
                     INGDNVKSAQIDDREQQVRLDLKNGNGDTGDSAKIITKYPTGYGVDLFNALNAKNAKT
                     NTVVNQGNIFGSLLIYLLPLLLLVGLFVMFSRMQTGGRMGFGFGKSKAKQLGKDMPKT
                     TFADVAGVDEAVEELYEIKDFLQNPTRYQALGAKIPKGVLLYGPPGTGKTLLAR"
     gene            864..1757
                     /locus_tag="VGP27_22595"
     CDS             864..1757
                     /locus_tag="VGP27_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854119.1"
                     /translation="MLTTTERLVHVNGMRLRVIEAGDRGAPVIVLAHGFPELAYSWRH
                     QIPVLADAGYHVLAPDQRGYGGSSRPDAVEDYDIHALTGDLVGLLDDVDVPQAVFIGH
                     DWGAMVVWHTALLHPDRVRAVAGLSVPPIPRARSRPTERWREKFGDDFYMLRFQEPGL
                     ADAEMEADVAVTMRGMFAGLIAGDAPLPGWISPGEFEHYVTAFSRTGFTGALNWYRNY
                     DRNWESTPQLADAKITAPAVFIGGTADPVGPTMNPARAREVAAGPYTAHTIEGAGHWV
                     QQERPEEVNRLLLGFLREVDR"
     gene            1757..2776
                     /locus_tag="VGP27_22600"
     CDS             1757..2776
                     /locus_tag="VGP27_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7854120.1"
                     /translation="MRASVLREGRMVLRDDVAEPVPAPGQVLVAVKACGICGSDLHFA
                     AHGDQMVRLTSEITGMPFGGGLRVDLADDVFMGHEFSAEVLEAGPDTDAPPPGTVVTS
                     LPVLLSPKGFEPILSSNTTIGGYAERMLLSAPLLLKIPNGLDPRLASLTEPMAVGLHA
                     VNKSGIRPGDDALVVGSGPIGLAIIGALRHRGVEHIVASDYSKTRRELATTMGAHQVV
                     DPAQGSPFDSVKPAVVFEAVGVPGIINDVMRRAPTGTRLVVAGVCMESDPIVPLFGIA
                     KEITVQFVNTYDATEFAESLRIIAEGEIDVAPLITGEVGLDGVGAAFEDLANPDTHCK
                     ILVTP"
     gene            2885..>4524
                     /locus_tag="VGP27_22605"
     CDS             2885..>4524
                     /locus_tag="VGP27_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854121.1"
                     /translation="MTNVEAKAADLYANDPQFRNARPIAEVIDAASHHNLRLTEVLQT
                     LVEGYADRPALGQRARELVTDIATGRTSVRLLPTFESISYREVWDRVAAIATALRNNA
                     TAPVKPGDLVATIGFASPDYFTVDLACAYLGLVSVPLQHNAPVSQLRPIIAETQPKIV
                     AVGAEYLDLAVESASASDSLKQLVVFDFNPAIDDHREAIERARIRFRDAGKPVVVETL
                     GRVIEQGSGLPTEPSFNGGSDDRLAMIMYTSGSTGAPKGAMITERTLATLFTSNFTPS
                     TGHPVFNVNFMPLNHIGGRLPIMSAFLAGGTSYFVAEPDLSTLFEDWALVRPTDMPLV
                     PRVVDMLFQRYRSAVDRVVAEGATAEDAEAEATTQLREHVLGGRVLAGVVATAPLAAE
                     MKTFIDTVLGAHITDGYGTTEVGGVTRDGVVMRPPVIDYKLVDVPELGYFHTDKPYPR
                     GELLVKTQTMMPGYYKRPDVTAEVFDADGYYRTGDVMAEVGPDHLAYVDRSKNVLKLS
                     QGEFVAVSNLEAVFAGAPLVRQIFVYGNSERPNVLAVIV"
BASE COUNT          792 a         1507 c         1436 g          789 t
ORIGIN      
        1 gcgggccagc agcgtcttgc cggtgccggg cggcccgtag agcagcacgc ccttggggat
       61 cttggcgccc agcgcctgat accgcgtcgg gttctgcagg aagtccttga tctcgtagag
      121 ctcctcgacc gcctcgtcga cgcctgccac gtcggcgaag gtggtcttcg gcatatcttt
      181 gcccagctgc ttggccttcg acttgccgaa cccgaagccc atccggccgc cggtctgcat
      241 gcgggagaac atcacgaaca ggccgaccag cagcagcagc ggcaacaggt agatcagtag
      301 cgacccgaag atgttgccct ggttgacgac ggtgttggtc ttggcgttct tggcgttcag
      361 cgcgttgaac aggtcgaccc cgtacccggt cgggtatttg gtgatgatct tggcgctgtc
      421 gccggtgtcg ccgttgccgt tcttcaggtc caggcgcacc tgctgctcgc ggtcgtcgat
      481 ctgagcgctc ttgacgttgt cgccgttgat ctgggccatc gccaccgacg tgtcgacggg
      541 cttgtagccg cgagtgttgt cactgaagta gaagaacgac cagcccagca acagcaccac
      601 cgcgatgacg gtgagcgtgc ggatcacatt tttgcggttc atcaagcatc ggccctatta
      661 ggccaagtcc ttccatatgc gcagctggaa tagtcaaggt taccgctaca ccaacgtccg
      721 gcggttcccg acggttctcc agcgccgttc gccagacgat gaagtcgagc gtgcggattc
      781 gtacgggaca cgccgctgcg ggtgtacgaa actgcacagt gggggctctt tcgagccagg
      841 tgggcctgtg cttgggtgaa gccgtgctca ccactaccga gcggctggtc cacgtcaacg
      901 gcatgcggct gcgggtcatc gaggccggtg atcgcggagc gcccgtgatc gtgctggcgc
      961 acggcttccc ggagctggcc tactcgtggc gacaccagat cccggtgctc gcggacgccg
     1021 gctaccacgt actcgcccca gatcaacgcg gctacggcgg ctccagccga cccgacgccg
     1081 tcgaggacta cgacatccac gccctgaccg gcgacctcgt cggcctgctc gacgacgtgg
     1141 acgtgccgca ggccgtgttc atcggccacg actggggtgc gatggtggtg tggcacaccg
     1201 cgctgctgca tcccgaccgc gttcgtgccg tcgcggggct cagcgttccg cccatccccc
     1261 gcgccaggtc ccggcccacg gaacgctggc gcgagaagtt cggcgacgac ttctacatgc
     1321 tgcgcttcca ggagccgggc ctcgccgacg ccgagatgga ggccgacgtg gccgtcacca
     1381 tgcgaggaat gttcgccggg ctgatcgcag gagacgcccc gctgcccggc tggatcagcc
     1441 ccggcgaatt cgaacactac gtaaccgcgt tctccaggac cgggttcacc ggtgcgctga
     1501 actggtatcg caactacgac cgcaactggg agtcgacgcc gcaactcgct gacgcaaaga
     1561 ttactgcgcc ggccgtcttc atcggcggta cggccgaccc ggtgggcccg acgatgaacc
     1621 cagccagggc tcgcgaggtg gccgcaggcc cctacaccgc gcacacgatc gagggagcgg
     1681 gtcactgggt gcaacaggag cgccccgagg aggtcaaccg actcctgctc ggcttcctcc
     1741 gggaagtcga ccgctgatgc gcgcctcggt cctgcgcgag gggcgaatgg tgctgcgcga
     1801 cgacgtcgcc gaacctgtgc ctgcaccggg tcaggtgctc gtcgccgtca aggcatgcgg
     1861 catctgcggc tcggatctgc acttcgccgc ccacggcgac cagatggtca ggctgaccag
     1921 cgagatcacg ggcatgccgt tcggaggggg tcttcgggtc gatcttgccg acgacgtctt
     1981 catggggcac gagttcagcg cagaggtgct cgaagccgga cctgacaccg acgcgccgcc
     2041 acccggaacg gtcgtcacgt cactccctgt attgttgtcg ccgaagggtt ttgagcccat
     2101 cctgtccagc aatacgacga tcggcggcta tgccgagcgg atgctgcttt cggcaccgct
     2161 gctgttgaag attcccaacg gtctcgaccc gcggctcgca tcgctcaccg agccgatggc
     2221 cgttggtctg catgcggtga acaagtccgg gatccggccc ggcgacgacg ccctcgtggt
     2281 cggatcgggt cccatcggcc tcgcgatcat cggcgccttg cgccatcgcg gtgtggaaca
     2341 cattgtggcg tcagactatt cgaagacccg gcgggagctg gcgacgacga tgggcgcaca
     2401 ccaagtcgtg gatcctgcac agggatcacc gttcgacagt gtcaagcctg cggtggtgtt
     2461 cgaggccgtc ggagtgcctg gaatcatcaa cgacgtgatg cggcgggcac cgacgggtac
     2521 ccgccttgtc gttgcgggag tgtgcatgga atccgatccc atcgtgcccc tgttcggcat
     2581 cgccaaggag atcaccgtgc agttcgtgaa cacctacgac gcaacagagt tcgccgagtc
     2641 actgcgcatc atcgccgagg gtgaaatcga cgtcgcgcca ctgattaccg gcgaggtggg
     2701 gttggacggc gtcggggccg cattcgagga cctcgccaac ccggatacac actgcaagat
     2761 cctggtaact ccctgagcgg agctggaatt ccgctgtgtg cggagcctca cagccgcgca
     2821 gcggaattcg ataacgccgc caggcgctgc ccctttagcg atgtggcgcg aaaggggccg
     2881 gatgatgacc aatgtagaag cgaaagcggc cgatctctac gccaacgacc cacagttccg
     2941 aaatgcccgg cccattgccg aagtcatcga tgcggcaagc caccacaacc ttcgcttgac
     3001 cgaggtcctg cagaccctcg tcgagggtta tgcagacagg ccggcgctcg gtcagcgcgc
     3061 acgggaactc gtgacagaca tcgccaccgg tcgcacctcg gtgcgcctgc tgcccacgtt
     3121 cgaatcgatc agctaccgcg aggtatggga ccgggtcgcc gcgatcgcca ccgcactgcg
     3181 caacaacgcc accgcaccgg tgaagcccgg tgatctcgtc gccacgatcg ggttcgcgag
     3241 ccccgactac ttcacggtcg atctcgcatg cgcatacctc ggcctggtgt cggtgcccct
     3301 gcagcacaac gcgcccgtct cccagctgcg cccgatcatc gccgaaaccc agccgaagat
     3361 cgtcgccgtc ggcgccgagt acctcgatct cgccgtcgaa tccgcatcgg caagcgactc
     3421 gttgaagcaa ctggtggttt tcgatttcaa ccccgccatc gacgatcacc gagaagccat
     3481 cgagcgggct cgcatccggt tccgcgacgc cggcaaaccc gtggtcgtcg aaacgctcgg
     3541 tcgtgtcatc gagcaaggct cgggtctgcc gactgagccg agcttcaacg gcggtagcga
     3601 tgaccggttg gcgatgatca tgtacacctc cggaagcacc ggcgcgccca agggcgcgat
     3661 gatcaccgag cgcaccctgg ccacgctctt cacctcgaac ttcaccccgt caaccggcca
     3721 cccggtgttc aacgtcaact tcatgccgct caaccacata ggcgggcggc tgcccatcat
     3781 gtcggcgttc ctggccggtg gtaccagcta tttcgttgcc gagcctgatc tttccaccct
     3841 gttcgaggat tgggcgctcg tgcgcccgac cgatatgccg ctggtgccga gggtggtcga
     3901 catgctcttt cagcgctacc gcagcgccgt cgaccgagtc gtcgccgaag gcgcgaccgc
     3961 cgaagacgcg gaagcggagg ccacaaccca actgcgcgag cacgtgttgg gcggacgggt
     4021 gctcgccggc gtggtcgcca ccgccccatt ggcggcggag atgaagacgt tcatcgacac
     4081 tgttctcggc gcgcacatca cagatggtta cggcacaacc gaagtgggcg gcgtgaccag
     4141 ggacggcgtg gtgatgcgcc cgcccgtcat cgactacaag ctcgtcgacg tccccgaact
     4201 cggctacttc cacaccgaca agccttaccc gcgcggcgaa cttctggtga agacgcagac
     4261 gatgatgccc gggtactaca agcgccccga cgtaacggcc gaagtgttcg acgccgacgg
     4321 ctactaccgc accggcgacg tgatggccga ggtcggaccg gatcacctgg cctatgtcga
     4381 ccgaagcaag aacgtactca agctctcgca gggcgagttc gtcgccgtct cgaacctcga
     4441 ggcggtcttc gccggagcac cattggtgcg gcagatcttc gtctacggca acagtgagcg
     4501 tcccaacgtg ctcgcggtga tcgt
//
LOCUS       DASYLX010000971         9519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3023574,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000971 DASYLX010000000
VERSION     DASYLX010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023574"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VGP27_22610"
     CDS             complement(<1..299)
                     /locus_tag="VGP27_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854122.1"
                     /translation="MSTVRAGADEGVTVNAEPTRRGQPDERQAREFQSDSLQRHAIEF
                     GEWMVRASNSRAHALGHRGLRPAHARLMVFLGWDGSRITDIAKAQDVSKNAVGQLV"
     gene            355..1275
                     /locus_tag="VGP27_22615"
     CDS             355..1275
                     /locus_tag="VGP27_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854123.1"
                     /translation="MVFAADELIPPATADPSGVWFFNWGIPIAGSIFIVLAIADVIRR
                     RRLTWGFLFLFNSIAVYWMETIGDWGQMLFYSPTFAEHHLLDWLPLKTPHDPLFMPFA
                     YAVYWGVHALLVLWLSQWVSSRTGWSMLKSMVVLAIPVNYIWDFLVEGTATAMGWWTY
                     DPGMGPVLEWDSGGRVTLLWTIGIMCTWPNLIAYWAGKPPIRGLNHLERFCRLDRLTV
                     PKKPVSTGAATDAAGLGSLAVATKPETLSKQQEFDAYLNYDVTIPRWRFELMRLGAWF
                     IGFQVSFAFLLVLPLVALRVVTGADSPYVP"
     gene            1272..1478
                     /locus_tag="VGP27_22620"
     CDS             1272..1478
                     /locus_tag="VGP27_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854124.1"
                     /translation="MSRKDVTDVNQNPPRHINMKDLPAEFFLPPQTNAALWSETVKLV
                     GGCVAFIVILGIVVALTQGIVVTL"
     gene            complement(1488..3074)
                     /locus_tag="VGP27_22625"
     CDS             complement(1488..3074)
                     /locus_tag="VGP27_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854125.1"
                     /translation="MPVDYRALRSRWYREGWFSERTCLHAFEAGAREYGLTPVVFATA
                     DAVDTVTVGDIHDRARALAAALQDLGTRSGDAVAVQLTNRFECAVAYQAVLLCGAVLV
                     PIVHIYGVAEVGFILAQSGARVLIVPERFRTTSYIERIPEFSRIDTLHHIIVLDADEG
                     DGYLSWSHLRASDEYRRPSVSADAVCLLLYTSGTTSAPKGVQHSHNTVLAEQRTLPAL
                     IAGAADDVSLVTFPPGHIAGVGNMFRPLMSGTRTVFLDGWNPARAVQLGHDYGVTSTA
                     GAPIHLQDLLDLGATGVPLPALREFLVGAAPVSEELGRRAAAAGIATFRSYGATEHPT
                     VTGEHAGEPEWARLATDGKPLPGSSVRILTSDGIEAPVGADGEVVVQGPEQFVGYRDA
                     ALNADAFTADGWFRTGDLGHVDPVGRLTITDRIKDVIVRGGETISSGQVEDVLNKHPA
                     VAEATAVAAPDARLGEVVAAVVVLKPGAALDIETLREHFAAAGLAKQKTPERLVFVDE
                     LPRTALGKVRKAELRRMHFG"
     gene            complement(3228..3701)
                     /locus_tag="VGP27_22630"
     CDS             complement(3228..3701)
                     /locus_tag="VGP27_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854126.1"
                     /translation="MPAITPSLWFDNNLEEAAEFYLSIFPNSSIESFERYTEAGPGTP
                     GHVVSGTFVLDGARFIGINGGPHFKFSEAVSFMVHCKDQAEVDYYWDRLVDGGEESQC
                     GWLKDRFGLSWQIVPDRLFELTSDPDPARAAAATKSMLGMRKIIVAELEEAVTNV"
     gene            3955..4266
                     /locus_tag="VGP27_22635"
     CDS             3955..4266
                     /locus_tag="VGP27_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854127.1"
                     /translation="MTIVSLVTIHPNPGVGWGDVQKQLKKACDIARKHGAENVTVLAT
                     MIGGAATNTIGLLSSAEDWEKFGKIQQSMMADPDMQAAMVDAGQIATWETYVSQTIPD
                     M"
     gene            complement(4281..4886)
                     /locus_tag="VGP27_22640"
     CDS             complement(4281..4886)
                     /locus_tag="VGP27_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854128.1"
                     /translation="MQPESTNADDRQEIIDAAYGCLSEPHSGPVPVAAILARAGLSTR
                     AFYRHFASKDELFLAMLRQEADALIGRLDGIADELPDTPVKQLTAWVEMMFDLAHDAE
                     LRTHFTVIDSDEVRAAKGYREMRERLHTDRERSLVRILERGRDGGSFPLAKPQQDAVA
                     ISAVVSRELATCAPATSEDLRRARTRVLDFALRALGARRSA"
     gene            4994..5881
                     /locus_tag="VGP27_22645"
     CDS             4994..5881
                     /locus_tag="VGP27_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854129.1"
                     /translation="MSTRRSEMQTVKFRGVDGLTLIADEWNRGAATAATSDTDIESDR
                     PSVLFLHGGGQNRYSWKGTGQILADEGLHVVALDSRGHGDSDRAADANYSVDALCADT
                     LAVLDQIGRPTILIGASMGGMTALPAAYEAGPEKVTKLVLVDVVPRYEKDGSARIREF
                     MSSGVNGFESLDEAAEAVAAYLPHRTKPRSTEGLKKNLRYRDGRWYWHWDPAFLTAPI
                     DDRFVRVEKLERAAINLSIPILLIRGRLSDVVSEEGVKHFLETVPRAEFVELSGAGHT
                     AAGDDNDAFSEVVVQFVNR"
     gene            5878..8835
                     /locus_tag="VGP27_22650"
     CDS             5878..8835
                     /locus_tag="VGP27_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEV7854130.1"
                     /translation="MNTARRTVGFNFLEEPELPSPQVTEGQAEQIVAQHYGLTARAKL
                     LGSNQDCNFLIYDTEDAVVGVLKVANPAFNATELEAQDAAADLVADAEPSLRIAVPMP
                     NLAGDKCTVITGLLDKPAYIRLLRFLPGGTLYESGYLPPEAVAGLGEVAGRVSRALAG
                     FEHPGLDRALQWDLRYGSDVVAQLLCHVADPSLRATVAEVTEAAWSRIRPLAESLPRQ
                     ATHLDLTDANVVATRRVGEAAHPDGVIDFGDLSHTWAVSELAITVSSVLGHVGSDPTS
                     ILPGIRAFHAIRPLSAEEAAVVWPLVVLRTAVLIVSGAQQAALDPDNAYVTEQTDGEI
                     RMFEQAASMPIDVMTAVIKADLGLADEPRDVVVDTALIEGLDPASVVTLDLASQSDAF
                     DSAFVPGGWLRADVESEFARAAVDDGAALVITRFGQPRLSRTTPLSHDSPEVVPTGIS
                     LWPAADIRAVAPWDGDVVEGSDDSVTLRAADYELTLTGVAPAPTPGTRLRAGDPLADA
                     RSGRWAEIGVRPVGAPRAPALTTAELSRGWLALSRDPRQVLGLPGLPETRAADDLLTR
                     RDESFAQVQEHYYAKPPQIERGWRHFLISTAGRSYLDMVNNVTVLGHAHPRVAETAAR
                     QLRKLNTNSRFNYEAVVEFSERLAAMLPEPLDTVFLVNSGSEASDLALRLAMTATGRR
                     DVVAVREAYHGWTYATDAVSTSVADNPNALATRPDWVHTVESPNSFRGKFRGADAGRY
                     ASEAVTQIEGLIADGRAPAAFIAEPVYGNAGGMALPDHYLEQVYAAVRAGGGLAVADE
                     VQVAYGRLGNWFWGFQQQNVVPDIVSVAKSTGNGSPLGAVITSRKIADRFRAQGYFFS
                     STGGSPLSCAVGITVLDALHDEGLQENAARVGAHLKARLQALQEKHQIIGTVHGIGLY
                     LGVEMVRDRSTLEPAVGETAAICNRMLELGVVIQPTGDQMNILKTKPPLCIDREAADF
                     YVDTLDRVLTEGF"
     gene            complement(8859..>9519)
                     /locus_tag="VGP27_22655"
     CDS             complement(8859..>9519)
                     /locus_tag="VGP27_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEV7854131.1"
                     /translation="DKSQLMAMTFALRHRWWVVLGGITVATTGVHLISVAVGHYLGAA
                     LPTHLLGILAGVAFVLFGLWTLRGDTLSDDEATRAQRSSAPAFFAVTSAFLLAELGDK
                     TMLATITLAADNDWVGVWIGSTLGMVAADALAILVGAIAGKHLPERLIQIAAAALFVV
                     FGVYMLVEGIFPVVPAVAVAAIALGAVLIIGAVLRALPDRLRPPVMRAKQSPTATSVD
                     V"
BASE COUNT         1713 a         3111 c         3096 g         1599 t
ORIGIN      
        1 accaattggc cgaccgcgtt cttcgagacg tcctgagcct tcgcgatgtc ggtgatacgg
       61 ctgccgtccc aaccgagaaa aaccatcaga cgggcatgtg cgggccgtaa acccctgtga
      121 cccaacgcgt gtgcgcggct attgctggca cgcaccatcc attcaccgaa ttcgatcgca
      181 tgccgctgca gcgagtccga ctggaattcg cgggcctgcc gctcgtcggg ctggccgcga
      241 cgggtgggct ctgcgttcac tgtcacccct tcatcggccc ccgcacgcac ggttgacatc
      301 acggcggatt ttagtgaagc tatcttcact atatcgcgtc gggaaaggcg gcaaatggtc
      361 ttcgcagcgg acgaactgat cccgcccgcc accgcggacc cgtccggcgt gtggttcttc
      421 aactggggga tcccgatcgc cgggagcatt ttcatcgtgc tggcgatcgc cgacgtaatt
      481 cgccgcagac gtctcacctg ggggttcctg ttcctgttca acagcatcgc cgtgtactgg
      541 atggagacga tcggcgactg gggccagatg ctcttctaca gcccaacgtt cgccgagcac
      601 cacctcctcg actggttgcc gctgaagacg ccccatgacc cgctgttcat gccgtttgcg
      661 tacgccgtct actggggtgt gcacgccctg ctcgtgctgt ggctgagcca gtgggtgtca
      721 tcgcgcacgg ggtggagcat gctgaaatcg atggtcgtgc tggccatacc ggtcaactac
      781 atctgggact tcctggtcga gggcaccgca actgcgatgg gctggtggac ctatgacccg
      841 ggcatgggtc ccgtgctcga atgggacagt ggtggccggg tcaccctgct gtggaccatc
      901 gggatcatgt gcacctggcc caatctgatc gcctactggg ccggcaagcc cccgatccgg
      961 ggtctcaacc acctggaaag gttctgccga ctcgaccgcc tcacggtgcc caagaagccc
     1021 gtttcgaccg gtgccgccac cgacgcggcg ggtctgggaa gcctcgctgt cgccaccaag
     1081 ccggagaccc tgtccaagca acaggagttc gatgcctatc tcaactacga cgtcacgatt
     1141 ccgcggtggc ggttcgaact gatgcgactg ggcgcctggt tcatcggatt tcaggtgagc
     1201 ttcgccttcc tgctggtgct gccgctggtc gcgttgcgcg tggtgaccgg tgccgacagc
     1261 ccgtacgttc cgtgagccga aaggacgtga ccgacgtgaa ccaaaatccg ccacgacaca
     1321 tcaatatgaa agacctcccc gccgagttct ttctgccgcc gcagaccaac gccgcgctgt
     1381 ggagcgaaac cgtcaaactc gtcggcggat gcgttgcgtt catcgtgatc ctcggcatcg
     1441 tcgtcgctct cacccagggc atcgtcgtca cgctgtagcg gacgtcgtca gccgaagtgc
     1501 atgcggcgaa gctcggcctt gcgcaccttg cccagcgccg tacgcggcaa ctcgtcgacg
     1561 aagacgagcc gctccggtgt tttctgtttg gccagcccgg cagccgcgaa gtgttcccga
     1621 agcgtttcga tgtcaagtgc agcaccgggt ttgaggacaa cgacggccgc gaccacctcg
     1681 cccagccggg catcgggagc ggccaccgcc gtggcttcgg ccaccgcagg gtgcttattg
     1741 aggacgtctt cgacctgacc cgatgagatc gtttcgccgc ctcggacgat gacgtctttg
     1801 atgcggtcgg tgatggtcag ccggccgacc ggatcgacgt gaccgagatc gccggtgcga
     1861 aaccagccat cggcggtgaa cgcgtcggcg ttcagggcgg catcgcggta accgacgaac
     1921 tgctcgggcc cctggacgac gacctcgcca tcggcgccca ccggcgcttc gatcccgtcg
     1981 gatgtaagga tgcgcaccga cgaaccgggc agcggtttgc cgtccgtggc caaccgcgcc
     2041 cactcgggtt cacccgcgtg ttcgcccgtc acagtgggat gctcggtggc gccgtaggaa
     2101 cggaacgtgg cgataccggc cgcggccgcc cgcctgccga gttcctcgct gaccggtgcc
     2161 gcaccgacga gaaactcgcg cagcgcgggc agcggcacac ccgtagcacc gagatcgagc
     2221 aagtcctgca ggtggatggg cgcacccgca gtagaggtga cgccgtaatc gtgccccagc
     2281 tggaccgcgc gagcgggatt ccatccgtcc aaaaacacgg tccgagtgcc cgacatcaga
     2341 ggccgaaaca tgttgccgac tccggcgatg tgccccggcg ggaacgtcac cagcgacacg
     2401 tcgtcggctg ccccggcgat cagcgcaggc agggttcgct gctcggcgag caccgtgttg
     2461 tggctgtgct gcacaccctt tggcgccgac gtggttcccg acgtgtacag cagcaggcag
     2521 acggcgtcgg cggatacgga tggccgccga tattcgtccg atgcccgcag atgcgaccac
     2581 gaaaggtatc cgtcgccctc gtcggcgtcg agcacgatga tgtggtgcag ggtgtcgatg
     2641 cgcgaaaact ccggtatgcg ttcgatatag gaggtcgtgc ggaagcgctc gggcacgatg
     2701 agcacccgtg cgccggactg ggcgaggatg aacccgacct cagccacgcc gtagatgtga
     2761 acgatcggaa ccagcaccgc accgcacagc agcactgcct ggtaggcgac ggcgcactcg
     2821 aaccggttgg tcaactggac cgcgacggca tccccgctgc gggtaccgag gtcctgcagt
     2881 gccgcggcca gcgcacgcgc gcggtcgtga atgtcaccga ccgtcaccgt gtcgaccgcg
     2941 tcggcggtgg cgaacaccac cggagtcaac ccgtattcgc gtgcgccggc ttcgaacgcg
     3001 tgcaggcagg tccgctccga gaaccagccc tcgcggtacc acctgctccg cagcgctctg
     3061 taatcgacgg gcatagatcg atgctataga tcctccgcag tgagcctctg acagaggcat
     3121 accttgcctt gtacgcccag ctatttacaa tgagatccat tgtgcatcag gatctttggc
     3181 ggcgctggag ccactacgcg tcgagaacgt gtgaaaggca gcccctctca cacgttcgtg
     3241 accgcttcct ccaactcggc gacgatgatc ttgcgcatcc cgagcatcga cttggtggca
     3301 gcggcggcgc gggccggatc tggatcgctg gtcagttcga acaaccgatc cggcacgatc
     3361 tgccagctca acccgaagcg gtccttgagc caaccgcact gcgactcctc gccgccgtcg
     3421 accagacgat cccagtagta gtcgacctcg gcctggtcct tgcagtgcac catgaacgac
     3481 accgcttcgg agaacttgaa gtgcggcccg ccgttgattc cgatgaaccg cgcgccgtcg
     3541 agcacaaagg tgccggagac gacatggccg ggcgttccgg gccccgcttc ggtgtagcgc
     3601 tcgaacgact cgatcgacga gttcgggaaa atcgaaaggt agaactctgc ggcttcctcg
     3661 agattgttgt cgaaccacag tgacggggtg atggcgggca ttggggcgac tccttcgcat
     3721 gggtgctact cgatacagac tctgccgccc gccaaaactc atccagggga agatgctgcg
     3781 ccggtcggcg aatggtcacg tcccagaact gtttgctatg tcaatcaaat ttgaatataa
     3841 aacctgatta tggatttttg gtgattttgc tgccaagtcg gtctggcggc gagaccagcg
     3901 gcctacagtc agcacgtcag acagccaacc tttaggcaag acgggggcac caccatgacc
     3961 atcgtttcgt tagtgacgat tcacccgaac ccgggcgtcg gctggggcga cgtgcagaag
     4021 caactcaaga aagcatgtga catcgcccgc aagcatggcg ccgaaaacgt caccgtcctg
     4081 gcgaccatga tcggcggggc agccaccaac accatcggcc tcctctcgag cgccgaggac
     4141 tgggagaagt tcggcaaaat tcagcagtcg atgatggccg accccgacat gcaggccgcc
     4201 atggtagatg ccggtcagat cgccacctgg gagacctacg tcagccagac gatcccggat
     4261 atgtagcgat gatccggaca tcaggctgac cgtcgggcgc ccaacgcccg cagtgcgaag
     4321 tcgagcacgc gggttcgtgc gcgccgcaaa tcctcagagg tggcaggtgc gcatgtcgcc
     4381 agctccctgc tgacgaccgc gctgatggcg acggcgtcct gctgcggctt cgccagcgga
     4441 aaggagccgc cgtcgcggcc gcgctcgagg atccgcacca gcgagcgttc gcggtcggtg
     4501 tgcaggcgtt cgcgcatctc ccgatagccc ttggctgccc ggacttcgtc ggagtcgatg
     4561 acggtgaagt gcgttcgcag ctcagcgtcg tgcgccaggt cgaacatcat ctccacccac
     4621 gccgtcagct gcttgaccgg agtgtcgggc agctcatcgg cgatgccgtc cagccggccg
     4681 atcaacgcat cggcctcctg ccgcagcatc gcgaggaaca actcatcctt cgatgcgaag
     4741 tgccgataga acgccctggt ggacaggccg gcgcgggcga ggatggcagc gacaggaacg
     4801 gggccgctgt gtggctcgga cagacagcca tacgcagcgt cgatgatttc ctggcggtca
     4861 tcggcattcg tcgactcggg ctgcacgctc gcagattgta gggataggcg ttctcagccg
     4921 tttggccacc atgtgccaac ggccgtattt gccctgggta acgtgaggcc cgggattggc
     4981 cgagaggacg ttggtgagca ctaggcgcag cgaaatgcag acagtgaagt ttcggggtgt
     5041 cgacgggctc acgttgatcg ccgacgagtg gaaccgcgga gccgccacgg cggcaacatc
     5101 ggacacggac atcgaatccg accgtccgtc cgtattgttc ctgcacggag gagggcagaa
     5161 tcgctactcc tggaagggca ccggccagat cctggccgac gagggactac acgtcgtcgc
     5221 gcttgactcc cgcggacacg gcgacagcga tcgtgccgcc gacgcgaact actcggtgga
     5281 cgcgctgtgc gccgacacgc tggcggtgct ggaccagatc ggcagaccca cgatcctgat
     5341 cggcgccagc atgggcggga tgaccgcact gcccgccgcg tacgaagcgg gtcccgagaa
     5401 ggtgacgaag ctggtgctcg tggatgtggt accgaggtac gagaaggacg gcagcgcgcg
     5461 gatccgcgaa ttcatgtcca gcggcgtgaa cgggttcgag tcactggacg aagcagccga
     5521 agcggtggcc gcataccttc ctcaccgaac caagccacgc agtacggagg ggctgaagaa
     5581 gaatctccgg taccgcgacg ggcgctggta ttggcactgg gatccggcgt ttttgaccgc
     5641 gcccatcgac gaccgcttcg tccgggtgga gaagctcgag cgggccgcga tcaacctgag
     5701 cattccgatc ctgctgattc gcggccggct atccgatgtg gtcagcgaag agggcgtgaa
     5761 gcatttcctg gagacggttc cccgcgccga gttcgtcgaa ctgtccggcg ccgggcacac
     5821 cgctgccggc gacgacaatg acgcattcag tgaagtcgtg gtccagttcg tcaaccggtg
     5881 aacactgcgc gccgcaccgt cggcttcaac ttcctcgaag aacctgagct gcccagccct
     5941 caggtgaccg aagggcaggc cgagcagatc gtcgcccagc actacgggtt gacggcacgg
     6001 gcgaagctgt tgggcagcaa ccaggactgc aacttcctca tctacgacac cgaggacgcc
     6061 gtcgtgggtg tgctcaaggt cgccaatcca gctttcaacg caacggaatt ggaagcgcag
     6121 gacgcggcgg ccgacctcgt cgccgacgcg gagccgtcgc tgcgcatcgc ggtgccgatg
     6181 cccaacctcg ccggcgacaa gtgcaccgtg atcaccgggc tgctcgacaa gcccgcctac
     6241 atccggctat tgcgtttcct gccgggcggc acgctgtacg aatcgggcta cctaccacct
     6301 gaggctgtcg cgggcctcgg tgaagtggcc ggtcgagtca gccgcgcgct ggccggattc
     6361 gagcatcccg gactcgatcg cgcgctgcag tgggatttgc gatacggctc agacgttgtg
     6421 gcgcaacttc tttgccacgt cgccgaccct tcgctgcgcg ccacggtcgc ggaggtgacg
     6481 gaggccgcct ggtcacgcat ccgcccgctg gccgagagcc tgccccggca agcgacgcat
     6541 ctcgatctga ccgatgccaa cgtcgtcgca acccgtcgcg tcggtgaagc tgcgcacccc
     6601 gatggcgtca tcgacttcgg cgacctgtcg cacacgtggg cggtgtccga attggccatc
     6661 accgtgtcgt ctgtgctcgg acacgttggc agcgatccga cgtcgatcct tcccggcatc
     6721 cgggcgtttc acgccatccg tccgttgagc gccgaggagg cagcagttgt ctggccgctg
     6781 gtggtgctgc gcaccgcggt gctgatcgtc agcggggcgc aacaggccgc cctcgaccct
     6841 gacaacgcct acgtcaccga gcagaccgac ggcgagatac ggatgttcga gcaggcggca
     6901 tcaatgccga tcgacgtgat gaccgcggtg atcaaggcgg acctcgggct ggcggatgag
     6961 ccccgcgacg tcgtcgtcga cactgcactg atcgaagggc tcgacccggc gagtgtggtg
     7021 acgctggact tggcgtcgca gtccgacgcg ttcgactcgg cgttcgtgcc cggcggctgg
     7081 ctccgcgccg acgtcgagag cgaattcgca agagccgctg tggatgacgg cgccgctctg
     7141 gtgatcaccc ggttcgggca gccccgcctg agccggacca ccccgctgag ccacgacagt
     7201 cccgaagtgg tgccgacggg catcagcctc tggcccgcgg cggacatccg cgccgtggcg
     7261 ccgtgggacg gtgacgtggt cgagggcagt gacgacagcg tcacgctccg cgctgccgac
     7321 tatgagctga cgctcaccgg tgtcgccccg gcgccgacac cgggcacccg gctgcgcgca
     7381 ggcgatccgc tcgccgatgc gcggtcgggc cgatgggccg agatcggtgt gcggcccgtc
     7441 ggtgcaccgc gtgcacctgc gctgacgacc gcggaactat cgaggggatg gctggctctg
     7501 agccgcgacc cgcgccaagt gctcggtctg cccggcctgc ccgaaacgcg tgccgccgac
     7561 gaccttttga cgcgacgcga cgaaagcttc gcgcaggtgc aggagcacta ctacgccaag
     7621 ccgccgcaga tcgagcgagg ctggcggcat ttcctgatat ccacggcggg ccgttcgtat
     7681 ctcgacatgg tcaacaacgt cacggtgctg ggtcacgctc atccgcgcgt cgccgaaacg
     7741 gcggcgcgtc agctacggaa gctgaacacc aactcccgct tcaactacga ggcggtggtc
     7801 gagttcagcg agcggctggc ggccatgctg cccgaaccgc tggacaccgt cttcctggtc
     7861 aattcgggtt cggaggcaag cgatctcgcg ctgcgcctgg cgatgacggc caccggaagg
     7921 cgcgatgtcg tcgcggtgcg cgaggcgtac cacggctgga cctacgcaac ggatgccgtc
     7981 tcgacgtcgg tggctgataa ccccaatgcg ctcgccacgc gcccggactg ggtgcacacg
     8041 gtggaatcgc ccaacagctt ccgcggcaag ttccgcggcg ccgacgccgg acgttatgcg
     8101 agcgaggcgg tgactcagat cgaaggtttg atcgccgacg gccgcgcgcc ggccgccttc
     8161 atcgcggaac ccgtgtacgg caacgccggc ggcatggcgc tacccgatca ctacctcgag
     8221 caggtgtacg cggcggtgcg cgcggggggc gggctggcgg tcgccgacga ggtccaggtc
     8281 gcttacggcc ggcttggcaa ctggttctgg ggctttcagc agcagaacgt cgtccccgac
     8341 atcgtgtcgg tcgccaaatc cactggcaac ggttctccgc tcggcgcggt gatcaccagc
     8401 cgcaagatcg ccgacaggtt ccgcgcccag ggctatttct tctcgtccac cggcggaagc
     8461 ccattgtcgt gtgcggtcgg catcacggtc ctcgacgcgc ttcacgacga gggattgcag
     8521 gagaacgccg cgcgggttgg cgctcatctc aaggcgagat tgcaagcgct gcaggaaaaa
     8581 caccagataa tcgggaccgt gcacggcatc gggttgtatc tcggtgtgga gatggttcgc
     8641 gacaggagca ctctcgaacc ggccgtcggg gaaacggcgg cgatctgcaa tcgcatgctc
     8701 gaactcggcg tggtgattca gcccaccggc gaccagatga acatcctgaa gacgaagccg
     8761 ccgctgtgca tcgaccgcga ggcggccgat ttctacgtcg acaccctgga ccgcgttctc
     8821 acagaggggt tctagggcgc accctttcag agggagcgct acacgtcgac agaagttgcc
     8881 gtcggcgact gcttcgcccg catcacaggc gggcgcagtc ggtccggcag tgcccgcagc
     8941 accgccccga tgatcaacac cgcaccaagc gcgatcgcag cgaccgccac agccggtacg
     9001 accgggaaga tgccctcgac gagcatgtac acaccgaaga cgacgaacag cgcggctgcg
     9061 gcgatttgga tgagccgctc gggaaggtgt ttacccgcga tcgcaccaac caggatggcg
     9121 agcgcatcgg cggccaccat gcccagcgtc gaaccgatcc acacacctac ccagtcgttg
     9181 tccgcggcaa gggtgatcgt cgcgagcatg gtcttgtcgc ccagttcggc gagcaggaac
     9241 gcggacgtga cggcgaagaa tgccggtgcg ctggatcgtt gcgcgcgcgt cgcctcgtca
     9301 tcggacagag tgtcacctcg cagcgtccac aatccgaaca ggacgaacgc cacaccggcg
     9361 aggatgccca gcaggtgggt cggcagcgcg gcacccaggt agtggcccac cgcgaccgag
     9421 atcaggtgta cccccgtggt ggcgaccgtg atcccgccga gcaccaccca ccagcggtga
     9481 cgcagcgcaa acgtcatcgc catcagctgc gacttgtca
//
LOCUS       DASYLX010000972         5341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2584823,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000972 DASYLX010000000
VERSION     DASYLX010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584823"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VGP27_22660"
     CDS             <1..945
                     /locus_tag="VGP27_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7854132.1"
                     /translation="VQGRAPLLYCRFHGWRYKLDGSLHSATRKDLLLDFDADSCRVPA
                     IQCEVWEGFIFVNLNPHNTEPVRSFLGELAHGIEGYPFAGPHQVYRFKAELQCNWKIF
                     LDGFAESYHGPYLHASSFGALTAEAREAFDQPNPFTDALAYQLKGPHRMFSFSGEPSR
                     KTPYSKPIECVFEASAAGPWNKKADRGPMPAGLNPTRSDKYGFDSYQFFPNFVLIFGA
                     SGFTVHTHWPTGPHSHIFETEAYYQPPTTHKERLGQELTMTFLNDIILEDASPSEGLQ
                     AMLNSGALTHFTMNDEEILLRHLHKTVADYVQAGEVGR"
     gene            942..1265
                     /locus_tag="VGP27_22665"
     CDS             942..1265
                     /locus_tag="VGP27_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854133.1"
                     /translation="MNATLPPDFSHLEHLVEQWAIEDGHQRYVKRVNSSMDQIKAFYD
                     EVFPVAEEAVAYIDKFDYSEPLPDDVANLRNLLYSLITVSLAVELWNQPRVKHSATTI
                     LTRVS"
     gene            1270..2100
                     /locus_tag="VGP27_22670"
     CDS             1270..2100
                     /locus_tag="VGP27_22670"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7854134.1"
                     /translation="MGLTSARLDIVDLTPRIGSEIRTDLETLLSGREAENIRATLEQR
                     GVVFFRGMEIDDEQQVAIANTLGAIPDNEGKGGIYKISLDKSVNQRAEYLKGSMFWHF
                     DGSLQPYPNLATLLRAITLSDTGGDTEFCNTYTAYDDLPQSDKDYIADLRVVHSAERS
                     QYYVRPEMCYDEITFWQKSPTKSCPLVWAHQSGRKSLLLGATADYVVGLPVEESRALL
                     ARLRDWATQPQYVYRHEWQPGDLLIWDNTGTMHRVLEYSVDSGRLMHRTILAGEEPLQ
                     "
     gene            2097..2951
                     /locus_tag="VGP27_22675"
     CDS             2097..2951
                     /locus_tag="VGP27_22675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7854135.1"
                     /translation="MKLAIATPVVTNVAGAPLTWERDASIEEVGRVAETADRLGYHHL
                     TCSEHIGIPATEAGRRGSRYWDPLATLGYVSARTTQIRLATMTLVLGYHHPLAIVKRY
                     GTLDHVSGGRVILGVGVGTLKQEFDLLGAPFDDRGDRADDALRALRAALSSNEPVYAG
                     KYYSFGGLTVDPCALQERMPIWIGGRTKRSLRRAVTLAEGWCPFGIPLDTAAEWLATR
                     ELPPGFEVVLPADQPFDPIKEPEATTETLAAMAAARTTIVSARFVHHSLEHYLEQIHA
                     LAEVHGSV"
     gene            complement(2948..3520)
                     /locus_tag="VGP27_22680"
     CDS             complement(2948..3520)
                     /locus_tag="VGP27_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854136.1"
                     /translation="MAIEERRERERAARRRLIITTARGLAEAEGWDAVTTRRLSTEIE
                     YSQPVLYKHFAGMEQLADAVAIDGFGELADVIRAACSATDTAGDTLVRVARAYLDFAR
                     DNPAVYDAMFTRATTLRFAAQDTPPELMAAFAALRQAVGLVADGQDADALTEVFWGAV
                     HGLVTLSRTGRLRPGYESERLQLLVDRFTG"
     gene            3621..4097
                     /locus_tag="VGP27_22685"
     CDS             3621..4097
                     /locus_tag="VGP27_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEV7854137.1"
                     /translation="MTLDLLTRAAAVIAVLGTAVVYGTDVFSAVVLRPALASVDDRAL
                     VSVMGGVHRYGDRRMPVPGVLGIVAAATSAVLAAVAAHWAQAIAAAAALVLLLVWVAL
                     YARVSAPINRQLTAAAEVDRPLPNGRALQAKWDRIIGARAILQGLGVAALCVVLAI"
     gene            4174..4413
                     /locus_tag="VGP27_22690"
     CDS             4174..4413
                     /locus_tag="VGP27_22690"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEV7854138.1"
                     /translation="MATFKAFGVTVHGVWTERSDGAHRLVALVSYPPSADPERHTRHI
                     MTSPEFAADMAGFDVNAIVDVQSLLLEPTPFSPIR"
     gene            4433..4813
                     /locus_tag="VGP27_22695"
     CDS             4433..4813
                     /locus_tag="VGP27_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEV7854139.1"
                     /translation="MTATTIAYILAGLIAAAIIFIGARFIVAPRAAAAGYGVQPDLGQ
                     RSAGAYLSVKGVRDIASGLFVIILMAAGASDLLGWVILAATIIPLADAAIVLGNGGPK
                     SIAWGVHGLTAAIMLTTSALLLVF"
     gene            complement(4810..>5341)
                     /locus_tag="VGP27_22700"
     CDS             complement(4810..>5341)
                     /locus_tag="VGP27_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854140.1"
                     /translation="DKTTIVDIARAADVSRSTVYEYFRDKAQIVEACAEGASQKFYRE
                     MASAMRKGETLEDQLVLAAVFVTRARRVIEPEDYFDEDEVSLLLTKNAAVLLRECTEF
                     LAPYLAAAKLTGEVRKDLDAVAAGEWYARMLFSLFSTPSASLDMDDARVVGEFVRAHA
                     VRGFAPDRPARGRLVP"
BASE COUNT          964 a         1776 c         1673 g          928 t
ORIGIN      
        1 gtgcagggcc gcgcgccgct gctgtactgc cgtttccacg gctggcgcta caagctggac
       61 ggctccctgc actccgccac ccgcaaggat ctactgctcg acttcgacgc cgacagctgc
      121 cgggtgcccg ccatccagtg cgaggtgtgg gagggcttca tcttcgtcaa cctcaacccg
      181 cacaacaccg aaccggtgcg gtcgttcctc ggtgagttgg cgcacggcat cgagggctat
      241 cccttcgcgg gtccgcacca ggtctacagg ttcaaggccg aattgcagtg caactggaag
      301 atcttcctcg acggcttcgc ggagagctac cacgggccgt atctgcacgc gtcctcattc
      361 ggcgccctga cagccgaggc gagagaggcg ttcgatcaac cgaacccgtt caccgacgcg
      421 cttgcatatc agctcaaggg tccgcaccgg atgttctcgt tctcgggcga gccgtctcga
      481 aaaacgccgt actcgaaacc gatcgagtgc gtattcgagg ccagcgcggc cggcccttgg
      541 aacaagaagg ccgaccgcgg gccgatgcct gccgggctca accccacccg gtccgacaag
      601 tacggcttcg actcctatca gttcttcccc aatttcgtgc tgatcttcgg tgcgtccggc
      661 ttcacggtgc acacgcactg gccgaccgga ccgcattcgc acatcttcga aaccgaggcg
      721 tattaccagc cacccacgac ccacaaggag cgattaggcc aggagctgac gatgacattc
      781 ctcaacgaca tcatcctgga ggacgccagc ccgtccgaag gtttgcaggc gatgctgaac
      841 agcggagccc tcacgcattt caccatgaac gacgaggaga tcctgctccg ccatctgcac
      901 aagacggtgg ccgactatgt ccaggcgggg gaggtgggac gatgaatgcg actctgccgc
      961 ctgacttctc gcacctcgag cacctcgtcg aacagtgggc gatcgaagac ggccatcaac
     1021 gctatgtgaa gcgcgtcaac agctccatgg accagatcaa ggcgttctat gacgaagtct
     1081 ttcctgtcgc ggaggaagcc gtggcctaca tcgacaagtt cgactattcc gaaccgcttc
     1141 ccgacgacgt ggcgaatctg cgcaacctgc tctactcgct gatcaccgtt tcgctggcgg
     1201 tcgaactgtg gaatcaaccg cgcgtgaagc attcggccac cacgattctc acgagagtga
     1261 gctgacgtca tgggactgac ctccgcacgg ctggacatcg tcgatctcac cccgcggatc
     1321 ggcagtgaga tcagaaccga cctcgaaacc cttctgagtg ggcgagaagc cgagaacatc
     1381 cgggcgacgc tcgagcagcg cggcgtggtc ttctttcgtg gcatggagat cgacgacgaa
     1441 caacaggtcg ccatcgccaa caccctcggt gcgattcccg acaacgaggg caagggcggc
     1501 atttacaaga tctccctcga caagagcgtc aaccagcgag ccgaatatct gaagggctcg
     1561 atgttctggc acttcgacgg ctctttgcag ccctacccga atctcgcgac gctgttgcgg
     1621 gccatcacgc tgtcggacac cggcggtgac accgagttct gcaacacgta cacggcatac
     1681 gacgacctgc cccaatcgga caaggactac atcgccgacc tgcgcgtcgt ccacagcgcg
     1741 gagcggtcgc agtactacgt ccgccctgag atgtgctacg acgagatcac gttctggcag
     1801 aagtcgccga cgaagtcgtg cccgctggtc tgggcacacc agtccggccg taagtcactg
     1861 cttctcggcg ccaccgcgga ttacgtggtc gggctgccgg tggaggagag ccgggccttg
     1921 ctcgcgcgcc tgcgtgactg ggccacgcag ccgcagtacg tctaccggca cgagtggcag
     1981 cccggcgacc tgctcatctg ggacaacacc ggcacaatgc accgggtgct ggagtattcc
     2041 gtcgacagcg gccgtctgat gcaccgtacg attctcgcgg gcgaagagcc gctgcagtga
     2101 aactcgccat cgccacaccg gtcgtcacca acgtcgccgg ggcgccactg acatgggagc
     2161 gagacgcgtc gatcgaggaa gtcggccgtg tcgccgagac tgcggatcgt cttggctacc
     2221 accatctcac gtgcagcgag cacatcggga tccccgccac cgaggcgggc agacgaggtt
     2281 cgcgctactg ggatccgctc gccacgctgg gctatgtatc ggcgcgcacc actcagatcc
     2341 ggctggcgac catgaccctt gtgctcggat atcaccatcc gctcgcgatc gtaaagcgct
     2401 acggcacact cgatcacgtc agcggcggcc gagtcattct cggcgtcggg gtgggcacgc
     2461 tcaagcagga gttcgacctc ctcggggcac cgttcgacga ccgcggcgac cgcgccgacg
     2521 atgcgctgcg ggcactgcgc gcggcactat cgagcaacga gccggtttat gccggaaagt
     2581 actactcatt cggcggtctc accgtcgacc catgcgcact gcaggagcgc atgccgatct
     2641 ggatcggcgg ccgaaccaaa cgctcgctgc ggcgcgcggt cacgctcgcc gaggggtggt
     2701 gcccgttcgg cattccgctc gacaccgccg cggagtggct tgcgacgcgg gaacttccgc
     2761 ccgggttcga ggtggtactg cccgccgacc aaccgtttga ccccatcaag gaacccgagg
     2821 cgacgacaga gacactggcg gccatggcag ccgcgcggac gacgatcgtc tccgcccggt
     2881 tcgtccacca ctcgctcgag cactacctcg agcagatcca cgcactggcc gaggtgcacg
     2941 ggtcggtcta accggtgaac cgatcgacga gcagttggag acgttcggac tcatagccag
     3001 ggcgtagccg gccggtacgg ctgagggtga ccagtccgtg cactgcaccc cagaacacct
     3061 cggtgagggc gtccgcgtcc tgcccgtcgg cgacgaggcc gaccgcttgg cgcaacgcgg
     3121 cgaaggccgc catcagctca ggcggtgtgt cttgggcggc gaagcgcagg gtggtcgcgc
     3181 gggtgaacat cgcgtcgtaa accgccgggt tgtcgcgggc gaagtcgagg taggcgcgtg
     3241 cgacccggac cagggtgtct cctgccgtgt cggtggcgga gcaggctgcc cggatcacat
     3301 cggccagctc gccgaagccg tcaatggcga cggcgtcagc gagttgctcc atgcccgcga
     3361 agtgtttgta caagacaggc tggctgtatt cgatctcggt ggacaaccgg cgggtggtga
     3421 cggcgtccca gccttcggcc tcggccagac cgcgggccgt tgtgatgatc agccgccggc
     3481 gggcggctcg ctcacgctca cggcgctctt cgatggccac agctcatgat agcaccgcta
     3541 taagtgctag cgctgctatt gacaccgtcg atcttagggg ttagcgttgc tagcaaccta
     3601 agccaaaagg gagtcaaaac atgacgctgg acctgcttac gcgtgccgcc gcagtgatcg
     3661 cggtgctggg taccgcggtg gtctacggca ccgacgtgtt ctccgcggtc gtgcttcgac
     3721 cggcattggc gtcggtcgac gaccgtgccc tggtctcggt catgggaggc gtgcatcgat
     3781 acggagaccg acggatgccc gtgccggggg tgctcggaat tgtggccgcc gcgacaagcg
     3841 cagtgctggc agcggtcgcc gcacactggg cacaagcgat cgccgcggcc gccgccttgg
     3901 tcctgctgtt ggtttgggtc gcgctctacg cacgggtgag cgcaccgatc aacaggcaat
     3961 tgacggcggc tgccgaggtc gatcgaccgc tgccgaatgg gcgtgcgctg caggcgaagt
     4021 gggaccggat catcggcgcc cgcgccattt tgcaaggtct gggagtggcc gccctgtgcg
     4081 tggtgctggc gatctgatgt ttcagctgag gatctacacc ctccgatcgc gcgaggctct
     4141 acagcagtac gcggtcattc attgggatcg cccgtggcaa ccttcaaggc attcggggtc
     4201 accgtccacg gcgtgtggac cgagcgaagt gacggcgcgc accggctcgt cgcgctggtc
     4261 agctacccac cgagcgccga ccccgagcga catacgcggc acatcatgac cagcccggaa
     4321 ttcgccgccg acatggccgg tttcgacgtc aacgcgatag tcgacgttca gtcgctgctg
     4381 ctcgagccga caccattctc accgattcgc taacaaccca ggagtgttcg acatgaccgc
     4441 cacgaccatc gcctacatac tcgccggatt gattgccgcg gccatcatct tcatcggtgc
     4501 acggttcatt gtcgcgccgc gcgccgccgc cgccggctac ggcgtgcaac cagatcttgg
     4561 tcagcgatcc gcaggcgcct acttgagcgt caagggtgtc cgcgacatcg catcggggct
     4621 gttcgtcatc atcctgatgg cggctggtgc ctcggacctg ctcggctggg tgatactggc
     4681 cgccaccatc attccgctcg ccgacgcggc catcgtgctc ggcaacggcg gccccaagtc
     4741 aatcgcctgg ggggttcatg gcctcaccgc cgcgatcatg ctgacgactt ctgcgctcct
     4801 gctcgttttc tagggaacaa gacggccgcg cgcaggccgg tcgggcgcga acccgcgcac
     4861 agcatgcgcg cgcacgaact cgccgacgac gcgcgcgtcg tccatatcca acgacgccga
     4921 cggggtgctg aacaacgaga acaacatccg cgcataccac tcgcctgcgg cgacagcatc
     4981 cagatctttt cgcacctcgc cggtgagctt cgccgcggcg agataggggg caagaaactc
     5041 cgtgcactcg cgcagcagca cggcggcgtt cttggtgagc agcaggctca cctcgtcttc
     5101 gtcgaagtag tcttccggct cgatcacgcg tcgggccctc gtcacgaaca ctgccgccag
     5161 aaccagttgg tcctccagcg tctcgccctt gcgcatcgca gaggccatct cgcgatagaa
     5221 cttctgcgaa gcgccctccg cgcaggcctc gacgatctga gccttgtcgc ggaagtactc
     5281 gtacaccgtg ctgcgcgaga catcggcggc ccgtgcgatg tcgacgatgg tcgtcttgtc
     5341 c
//
LOCUS       DASYLX010000973         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1600027,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000973 DASYLX010000000
VERSION     DASYLX010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600027"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VGP27_22705"
     CDS             complement(<1..651)
                     /locus_tag="VGP27_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854141.1"
                     /translation="MVRAQPTHSTRHDQPLRRPRALIVGLSIVVLTVAVLQTAVVPVL
                     GIIADQLNSSTVGVSWAVTANLLAAAAATPLIGRLADLHSKKRVLLVVLAVVLVGSIL
                     AATTSSLTLLIVGRILQAASYALYPVGVAILREELTEDRMVSAMSVLSGTLGFGGGTG
                     LVVVGLLVNHGADYHRVFWLTTAFTVIVIAIVVLAVPARPRSATGSIDWLGAAGLAA"
     gene            complement(691..>1671)
                     /locus_tag="VGP27_22710"
     CDS             complement(691..>1671)
                     /locus_tag="VGP27_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV7854142.1"
                     /translation="VTAILPRGRAGVGEPCAAGWYSLNGNGEMTGTTWIEEAGSFDLP
                     VVLSNTHAVGACHTGVIRWVNRVDPRLARQWLLPVCAETWDGYLNDINGGHVRPEHVE
                     AALDAATTGAVGEGSVGGGTGMNCYGYKGGNGTASRLVRYGEATFTVGTFVQANFGSR
                     AELTVAGRLIGPELLDDNPLDGDWFEVDLGLKPPPGAGSVIAVVATDAPLLPGQCKAL
                     ARRVPLGLARTGTAGSHFSGDIFLAFSTADVPGLASAFPVGPAGVDEFGTISFVPWGR
                     IDDLYTAVVQSVEEAVLNALIVNDDMVGRDGHRSPRLPLERLSALMDART"
BASE COUNT          293 a          561 c          568 g          249 t
ORIGIN      
        1 ggccgccagg ccggcggcgc cgagccagtc gatcgacccc gttgcgctgc gcggccgtgc
       61 gggcaccgcg aggaccacga tggcgatcac gatgacggtg aaggcggtgg tcaaccagaa
      121 gacgcggtga tagtcggcgc catggttcac cagcaggccg acgaccacga gtccggtgcc
      181 gccgccgaag ccaagcgttc ccgacagcac cgacatcgcc gaaaccatgc ggtcttcggt
      241 caactcctcg cggaggatcg cgacgccgac cggatagagc gcgtacgagg cggcctgcag
      301 tatccgcccc acgatcagca gcgtcagcga tgaggtggtg gcagcgagta tcgaacccac
      361 cagcaccacg gcaagcacga ccaacagcac gcgtttcttg ctgtgcaggt cggcgaggcg
      421 gccgatcaac ggcgtggccg ccgcagcggc gagcaggttc gccgtgaccg cccagctcac
      481 tccgacggta gagctgttga gttggtcggc gatgatgccg agcacaggaa cgaccgcggt
      541 ctgcaggacg gcgaccgtca ggacgacgat gctgagcccg acgatcagcg cccgtggtcg
      601 tcgaagaggc tgatcgtgac gtgtggagtg tgtcggttgc gccctgacca ctccatgctc
      661 cgatcgttag ctcgtcttcg gttccgtcga ttatgtcctt gcgtccatca gcgccgacaa
      721 ccgctcgagt ggaaggcgcg gcgagcggtg gccgtcgcgg cccaccatgt cgtcgttgac
      781 gatcagcgcg ttgagcaccg cttcctcgac ggactgcacg acggcggtgt agagatcgtc
      841 gatccggccc cacggaacga aagagatggt gccgaactcg tcgacgccgg ccggaccgac
      901 cggaaacgcg ctcgccagac cgggcacgtc cgctgtcgag aaggccagga agatgtcacc
      961 ggagaaatgg ctgcctgcgg ttccggttcg ggccagtccc agcggaacac gtctggcgag
     1021 tgccttgcac tgaccgggca acaggggcgc atcggtggcg acgacggcga tgacagatcc
     1081 cgcgcccggt ggtggcttga gtccgaggtc gacctcgaac cagtcgccgt ccagcggatt
     1141 gtcgtcgagc agttcggggc cgataagcct tcctgcaacc gtcaattcgg cgcgagaacc
     1201 gaagttggcc tggacgaagg tgcccacggt gaaggtggcc tcgccatagc ggaccaacct
     1261 cgatgcggtg ccgttgccgc ccttgtaccc atagcagttc atgcccgtgc cgccgccgac
     1321 cgagccctcg ccgaccgcgc ccgtggtggc ggcgtccagc gccgcctcga cgtgctccgg
     1381 gcggacgtgg ccgccgttga tgtcgttgag atagccgtcc caggtctcgg cgcacacggg
     1441 cagcagccac tggcgcgcga gccgcgggtc gacccggttg acccaccgaa tgacgccggt
     1501 gtggcaggcg cccaccgcat gcgtattcga cagcacaacc ggcagatcga acgacccggc
     1561 ctcctcgatc caggtggtgc cggtcatctc gccgttgccg ttgagcgaat accagccggc
     1621 cgcacacggt tcgccgaccc cggcgcggcc tcgcggcaat atcgcggtga c
//
LOCUS       DASYLX010000974         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2695389,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000974 DASYLX010000000
VERSION     DASYLX010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695389"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..457
                     /locus_tag="VGP27_22715"
     CDS             2..457
                     /locus_tag="VGP27_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV7854143.1"
                     /translation="MKWSFAQIGLLIIAAFHVIQAVIGLIVEPSFATGPDAPTAQVLG
                     MDYNGWHAVAGLALFAPGLVFALRKSWAVLYLLIAAVAGALPGIWAFFSHQVAFVFTF
                     PNNTTDAVVHLVSAAVMIAVAAIQIRIDGGLRNSLADLHRDVQTTRNTG"
     gene            complement(433..816)
                     /locus_tag="VGP27_22720"
     CDS             complement(433..816)
                     /locus_tag="VGP27_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854144.1"
                     /translation="MGAIAFAAGVGPRHAATLEVRGHKTGRTIGFPVVVADYRGGRYL
                     VAMLGQKTKWAANVRADDHAVLLMRRRRENVLLVEDFSGDRAEILRRYLDLAPGARPF
                     FPIDRRAAVRDFERIVNDYPVFRVV"
     gene            complement(902..1549)
                     /locus_tag="VGP27_22725"
     CDS             complement(902..1549)
                     /locus_tag="VGP27_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV7854145.1"
                     /translation="MLSVNLARVRPNPDAPSRSTGIDKAAAHDDVMVRPPGSMRGGLG
                     SGLVGDTIGNRKLHGGDDQAVYAYAREDLDEWESVLARRLINGMFGENLTTSGADVTG
                     ARIGERWRIGSDGPLLEVSAPRTPCRTFSAFLGLHNWIKTFTEAAKPGAYFRVISPGT
                     VRAGDAISIDYRPDHNVTIGLVFRARMSDPALLPHLLEVDALSSELKAYARRRVG"
     gene            1700..>2109
                     /locus_tag="VGP27_22730"
     CDS             1700..>2109
                     /locus_tag="VGP27_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7854146.1"
                     /translation="MKVRFGVGLGADPGPEQLAEIVDHLEAVDIDSLWFSELVYSPLV
                     EPFVGMAFALARTTRLKVGTSVAVLPGRHPVLVAKQLASLSALAPKRVLPVFGLRSAL
                     PAERDLFVVPDGKRAAVFDESLRLLRIALREDDVT"
BASE COUNT          340 a          687 c          701 g          381 t
ORIGIN      
        1 gatgaagtgg agtttcgccc agatcggcct gctgatcatc gcagcgtttc acgtcattca
       61 ggcggtcatc ggattgatcg tcgaacccag cttcgcaacc ggacccgacg cgccgaccgc
      121 ccaggtgctg gggatggatt acaacggctg gcatgcggtc gccggtctcg cgttgttcgc
      181 acccggcctg gtgttcgcgc tgcgcaagtc gtgggcggtg ctgtacctgc tcatcgccgc
      241 ggtggccggc gcgctgcccg gcatctgggc cttcttctca caccaggtgg ctttcgtctt
      301 cacctttccg aacaacacca ccgacgcggt cgtccacctg gtgagcgcgg cggtcatgat
      361 cgcggtcgcc gcgatacaga tccgcatcga cggcgggctg aggaattcgc tcgccgacct
      421 tcaccgggac gttcagacca cccgaaacac gggatagtcg ttcacgatcc gctcgaagtc
      481 gcgcactgcc gcgcggcggt cgatcgggaa gaaggggcgc gcaccgggag cgaggtcgag
      541 ataccggcgc aggatctcgg cccgatcgcc cgagaagtcc tcaaccagta agacattctc
      601 gcgccggcgg cgcatgagca ggacggcgtg gtcatcggcg cgaacgtttg ccgcccactt
      661 cgtcttctgg ccgagcatgg ccaccagata gcggccgccc cggtaatcgg cgacgacgac
      721 ggggaatccg attgtgcgcc cggttttgtg accgcgaact tcaagggtgg ccgcatgacg
      781 gggaccgacg cccgccgcaa aggcgatggc gcccaaccga ttcgagaccc gcgccgtgaa
      841 gttcggccga ccgccgcgat agttccaccg ctgcagggcg cttagtgcat cgcgcagcat
      901 gtcatcccac ccggcgccgc gcataggcct tgagctcgga ggaaagtgcg tcgacctcca
      961 acaggtgcgg cagcagagcc ggatccgaca tccttgcccg gaacaccagg ccgatcgtca
     1021 cgttgtggtc gggccggtag tcgatggata tcgcgtcgcc tgcgcgcacc gttccaggtg
     1081 agatgacacg aaaataggcg ccgggtttcg ccgcctcggt aaaggtcttg atccagttgt
     1141 gcagacccag gaaggccgag aaggtcctgc atggggttcg aggtgccgac acctcgagca
     1201 gcgggccgtc gctgccgatg cgccaccgtt cgccgatccg cgccccggtg acgtcggctc
     1261 ccgacgtggt gaggttctca ccgaacatgc cgttgatcag ccggcgtgcg agcaccgact
     1321 cccattcgtc gaggtcctct cgggcgtagg cgtaaacggc ttgatcgtcg ccaccgtgaa
     1381 gtttccggtt gccgatggtg tctccgacga gtccgctgcc cagaccaccg cgcatggatc
     1441 ccggcggccg gaccatcaca tcgtcgtgcg ctgcggcttt gtcgataccc gtcgacctcg
     1501 acggtgcgtc gggattcggg cggacgcggg cgaggttgac cgacaacaga gatgccacgc
     1561 gtcaaaggat attggttggg caacgaggcc gcttttcgac tgcatccgac cggctccgcg
     1621 caatccgtgg ccgcctgcgg cacaaacggc gcccctcgcg tgttgcgatg gggtagaccg
     1681 ttgatgcgag gaggcgcgag tgaaggtccg tttcggagtg gggcttggag ccgatccagg
     1741 tcccgagcaa ctggccgaga tcgtcgatca cctcgaggcc gtcgacatcg actcgctgtg
     1801 gttctccgaa ctggtctact cacctttggt cgagccgttc gtcggcatgg cgttcgcgct
     1861 cgcgcggacg acgcgattga aggtgggcac ctcggtggcg gtgctgcccg gtcgtcaccc
     1921 cgtgctggtc gccaaacagc tcgcatcgct gtccgcgctc gcgcccaagc gggtactgcc
     1981 cgtcttcggc ctgcggtcgg cgttgcccgc cgaacgtgac ctgttcgtcg tgccggacgg
     2041 aaagcgcgct gccgtcttcg acgagtcact gcgcttgttg cgcatcgcgt tgcgggagga
     2101 cgatgtgac
//
LOCUS       DASYLX010000975         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3024577,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000975 DASYLX010000000
VERSION     DASYLX010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024577"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGP27_22735"
     CDS             <1..811
                     /locus_tag="VGP27_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854147.1"
                     /translation="NGVRFHVTEAGPPDGRPVLALHGWPQHHWEYRDLLADPPPGLRI
                     IAPDLPGYGWSGPATHKWAKDDVATDVLALLDALGLDRVLLVAHDWGAAVGYFMVLRE
                     PERFDGYLVCNMAHPWLSTRDVLPHLWRFCYQLPLATFGIAVQRRTPYVERVIYTLGS
                     RMDRASAKVFAERFRDPVCAKAGRDTYRTFLTREIPQRARNPETRRITIPIRVLYGTG
                     DFAVARSFVDPETAHADDYTLETVDASHFVVDERPDLVRKKLIALAEETAH"
     gene            complement(827..1507)
                     /locus_tag="VGP27_22740"
     CDS             complement(827..1507)
                     /locus_tag="VGP27_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7854148.1"
                     /translation="MTRVLVVDDEPQILRALRINLSVRGYEVVTAASGSGALRAAADD
                     HPDVVILDLGLPDMSGIDVIGGLRGWLTAPIIVLSARTDSADKVEALDAGADDYVTKP
                     FGMDEFLARLRAAIRRGAAAAETDEPVIETASFTVDLASKKASKNGTEVHLTPTEWGM
                     LEMLVRNRGKLVGREELLKEVWGPAYAKETHYLRVYLAQLRRKLEDDPSHPVHLLTEA
                     GMGYRFQE"
     gene            complement(1504..>2074)
                     /locus_tag="VGP27_22745"
     CDS             complement(1504..>2074)
                     /locus_tag="VGP27_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7854149.1"
                     /translation="LATIEESIDQLTALVGNLLDSSRLAAGVVRPALRRVYLEETVQR
                     ALVGISQHSTGFRSLALDRVKVEVDDAVAMADSGLLERVLANLIDNALRYAPDSPVRV
                     TAGPVGDRVLIAVIDEGPGIPRGTEEQLFAPFQRLGDQDNNTGVGLGLSVAKGFVEAM
                     GGTVQATDTPGGGLTVLVDLAAPPRSSAT"
BASE COUNT          316 a          721 c          699 g          338 t
ORIGIN      
        1 caacggtgtg cgcttccatg tcacggaggc gggcccgccg gacggccgcc cggtcctcgc
       61 gctgcacggt tggccgcagc accactggga ataccgcgac ctgctggccg atccgccgcc
      121 agggctgcgg atcatcgcgc cggacctgcc cggttacggg tggtcggggc cggcgacgca
      181 caaatgggcc aaggacgacg tcgccaccga tgtgctggcg ctgctcgacg cactcggact
      241 ggaccgggtg ctgctggtgg cccacgactg gggtgccgct gtcggctact tcatggtgtt
      301 gcgcgagcca gagcgattcg acggttatct cgtctgcaac atggcccacc cgtggctgtc
      361 cacccgcgat gtgctgccgc acctgtggcg cttctgctac cagctgccgc tcgccacgtt
      421 cgggatcgcc gtgcagcggc gcactcccta cgtcgagcgg gtgatctaca ctctcggatc
      481 caggatggat cgggcatcgg ccaaggtctt cgccgaacgg ttccgtgacc cggtgtgcgc
      541 gaaggcgggc cgcgacacct atcgcacgtt tctgacacgt gagatcccgc aacgggcaag
      601 gaatcccgag acccggcgca tcacgattcc gatccgtgtc ctttacggga cgggcgactt
      661 cgctgtcgcg cggtcattcg tcgacccaga gaccgcccac gccgacgact acacgctcga
      721 gacggtcgat gcctcccact tcgtcgtcga cgagcggccg gatctggtgc gcaagaagct
      781 gatcgccctc gccgaggaga cggctcacta acaccggcgg agaaagttac tcctggaagc
      841 ggtagcccat gccggcttcg gtcagcaggt gcacggggtg cgacgggtca tcctccagct
      901 tgcgccgcag ctgcgcgagg tagacccgca gatagtgcgt ctccttggcg tacgcgggcc
      961 cccacacctc cttgaggagc tcctcgcggc cgaccagctt gccgcggttg cgcaccagca
     1021 tctccagcat gccccactcg gtcggcgtga ggtggacctc ggtgccgttc ttggaagcct
     1081 tcttcgatgc cagatccact gtgaacgagg ctgtttcgat gacgggctcg tcggtttccg
     1141 cggcggcggc accacgccgg atggctgcgc gcagacgcgc gaggaactcg tccataccga
     1201 atggcttggt gacgtagtcg tcggccccgg cgtcgagagc ctcgaccttg tcggcggagt
     1261 cggtgcgcgc cgacagcacg atgatcggcg cggtcagcca accccgcagg ccgccgatga
     1321 cgtcgatgcc cgacatgtcg ggcaacccga ggtcgaggat gacaacgtcg gggtggtcgt
     1381 ccgcggcggc gcggagtgca ccgctgccgc tcgcggcggt gacaacttcg tagccccgca
     1441 cggacaggtt gatgcgcagg gcgcgcagga tctgcggttc gtcgtcgaca accaaaaccc
     1501 ttgtcatgtc gccgaactcc tcgggggagc ggcgaggtcg acgagcacgg tcaatccgcc
     1561 gccgggcgtg tcggtggcct ggacggtccc gcccatcgcc tcgacgaatc ccttcgcgac
     1621 cgagaggccg aggccgacgc cggtgttgtt gtcctgatcg ccgagccgct ggaacggcgc
     1681 gaacaactgc tcctcggtgc cgcgcgggat gcccgggccc tcgtcgatca cggcgatcag
     1741 cacgcggtcg cccactgggc ccgccgtgac ccgcacgggg ctgtcgggcg cgtagcgcag
     1801 ggcgttgtcg atcaggttcg ccagcacccg ctcgagcagg ccgctgtcgg ccatcgcaac
     1861 cgcgtcgtcc acctccacct tcacccggtc gagcgccagg ctccgaaaac cggtggagtg
     1921 ctgactgatt ccgaccagcg cccgctgcac cgtctcttcc aggtagaccc gccgcagcgc
     1981 cgggcggacc actccggccg ccagtcgcga cgagtcgagc aggttgccga cgagcgcggt
     2041 gagctggtcg atcgactcct cgatcgtggc gagc
//
LOCUS       DASYLX010000976         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2804889,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000976 DASYLX010000000
VERSION     DASYLX010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804889"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VGP27_22750"
     CDS             complement(<1..178)
                     /locus_tag="VGP27_22750"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777222.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7854150.1"
                     /translation="MPEEAFIYEAIRTPRGKNKNGALNEVKPINLVTGLVDELRRRFP
                     DLDENMISDLVLGVV"
     gene            complement(361..1467)
                     /locus_tag="VGP27_22755"
     CDS             complement(361..1467)
                     /locus_tag="VGP27_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEV7854151.1"
                     /translation="MAFTLSENSALLRPVTVGDVPVANRLFMAPLTRSRAEPDATPSE
                     LAGLYYSQRAAAGLIISEATAVSAAANGAYLNTPGMFTDRHQRRWAEIADAVHESGGR
                     MFVQLWHVGRMGHPEISGVETVAPSAVAADLSAHTPTGRRSLPVPRALRTAEVTGIVA
                     DFRAAARRAVDAGMDGVEIHSANGYLLHEFLSDVTNRRDDAYGGSAHNRARLTAEVVE
                     AVADEVGAGRVGLRISPGNGAGDMREVDEIAAYEALLNRIAPLGLAYLHLLIEPTQPA
                     FAVVRAVWEGTLVLNTPREIQTNFCELENLVDWGVIGAAAVGRSYLANPDLIDRLTLG
                     ADLNEPDGATLYSSGPAGYTDYPTLPDAPSLEVA"
     gene            1522..>2228
                     /locus_tag="VGP27_22760"
     CDS             1522..>2228
                     /locus_tag="VGP27_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7854152.1"
                     /translation="MTVPNVAGRAATVKRLQPYAVTVFAEMSALAARVGAVNLGQGFP
                     DEDGPPAMLKTAENAIAEGVNQYPPGMGIAPLREAIAAQRNRQFGTEYDPDTEVLVTV
                     GATEAIAASVLGLVEPGSEVLLIEPFYDSYSPVIAMAGCERRAVPMRPDGRGFTIDVE
                     GLRRAVTPRTRALILNSPHNPTGMVASDAELRALAKLAVDADLLVITDEVYEHLVFDG
                     RRHLPLANYPGMAGRTVT"
BASE COUNT          355 a          740 c          769 g          364 t
ORIGIN      
        1 acaccacgcc gaggacgagg tcgctgatca tgttttcgtc gagatcgggg aatcggcggc
       61 gcagctcgtc gaccaggccg gtgaccaggt tgatgggctt gacctcgttg agggcgccgt
      121 tcttgttctt gccgcgaggc gtgcgaatcg cctcgtagat gaaggcttct tcaggcatga
      181 agatgttcct gtccagacgg ggttggttgg gagacctgcc cacatgggcg gttgtcctct
      241 acgcgccagc ctaacagcgc ctcccaactc gttggttggg aggttcgtca cgcgcccaaa
      301 ctcacatttg ggcgagaaag ttcgagtgga acccgccaat atgtcgatct cgacgcggcg
      361 tcaggcgact tcgagcgacg gcgcgtccgg cagcgtcgga tagtcggtgt agccggccgg
      421 ccccgaggag tacaacgttg cgccgtcggg ctcgttgagg tcggcaccga gcgtgaggcg
      481 gtcgatgaga tcggggttgg cgagatagga gcggccgacg gcggctgcgc cgatgacgcc
      541 ccagtccacg aggttctcca gctcgcagaa gttggtctgg atctcgcgcg gagtgttgag
      601 cacgagcgtg ccctcccaca cagcgcgcac gacggcgaac gccggctgcg tcggctcgat
      661 caacagatgc aggtatgcca accccagcgg cgctatccga ttgagcaacg cctcgtaggc
      721 cgcgatttcg tcgacttcgc gcatgtctcc cgcgccgttt ccgggcgaga ttcgcaggcc
      781 gacccgtccg gcaccgacct cgtcggcgac ggcttcgacc acttcggcgg tcaacctggc
      841 gcggttgtgg gccgacccgc cgtaggcgtc gtcgcgccgg ttggtcacat cggaaaggaa
      901 ctcgtgcagc agatagccgt tggccgagtg gatctcgacg ccgtccatgc ctgcgtccac
      961 ggcgcggcgc gcggccgcgc gaaagtcggc gacgatgcct gttacctcgg cggtgcgcag
     1021 agcacgcggc accggaagcg atctcctgcc cgtcggcgtg tgtgccgaca ggtcggcggc
     1081 gacggcagac ggggcgaccg tctcgacacc gctgatctcg ggatgtccca tccgcccgac
     1141 gtgccacagt tgaacgaaca tccggccgcc ggactcgtgc acggcgtcgg cgatctcggc
     1201 ccaccgccgc tgatgccgat cggtgaacat gccgggcgtg ttgaggtatg cgccgttggc
     1261 tgccgctgac accgcggtcg cctcgctgat gatgaggccc gccgcggcgc gctgcgagta
     1321 gtagagcccg gccagctccg acggcgtcgc gtcgggttca gcgcggctgc gggtcaacgg
     1381 agccatgaac agtcggttgg caacggggac gtcgccgacg gtgacgggac gcagaagtgc
     1441 cgagttctcg ctgagggtga acgccatgtc gcactcagcg cagcgaaggc ggctttcatt
     1501 cctggcctac gctcacaacc catgacggtg cccaatgtcg caggccgcgc ggcgacggtg
     1561 aagcggctgc agccctatgc cgtgacggtc ttcgccgaga tgtccgcgct cgcggcccgc
     1621 gtcggcgcgg tgaatctggg tcagggtttt cccgacgagg acggcccacc cgcgatgctc
     1681 aagaccgccg agaacgcgat cgccgaaggc gtcaaccagt acccgcctgg catgggcatc
     1741 gcgccgctgc gcgaagcgat cgccgcacag cgaaaccggc agttcggcac ggagtacgac
     1801 ccggataccg aggtgctggt caccgtcggc gccaccgagg cgatcgccgc gtcggtcctc
     1861 gggctcgtcg agccgggctc cgaggtgctg ctgatcgaac cgttctacga ctcctactcc
     1921 cccgtcatcg cgatggcagg ctgcgagcgc cgcgccgtgc cgatgaggcc cgacggccgc
     1981 ggcttcacga tcgacgtcga aggcctgcgg cgggccgtga ctccccgcac cagggccttg
     2041 atcctcaact cgccgcacaa cccgacgggg atggtggcct ccgacgccga actgcgggcg
     2101 ctggcgaagc tcgcagtgga cgccgaccta ctggtcatca ccgacgaggt ctacgagcat
     2161 ctggtgttcg acggccgccg gcatctgccg ctggccaact atcccggcat ggcgggccgg
     2221 accgtcac
//
LOCUS       DASYLX010000977         8582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1159313,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000977 DASYLX010000000
VERSION     DASYLX010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159313"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGP27_22765"
     CDS             <1..406
                     /locus_tag="VGP27_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mammalian cell entry related domain protein"
                     /protein_id="HEV7854153.1"
                     /translation="FPSFADAMIDAGYYATHGTNKLNKGTWNASEAEYQTDILPFMQY
                     VSDSLFGAIGRLEYTHIDDLTPMVDSVRALTDVVPPLMRPQGMSEMLVELRSRLEKMY
                     GGTPEQRALQVRIVLDSLPGVAAPLGAVGGAG"
     gene            403..1455
                     /locus_tag="VGP27_22770"
     CDS             403..1455
                     /locus_tag="VGP27_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854154.1"
                     /translation="MKPRAALWRLAISGIVAIALFIALANVIKQPTAAETRSYTAEFT
                     DASGLHPDADVRVRGVRVGKVQSVDLERRNGQSIAVVGLTCDQRYGVVSGTRLAIKFE
                     ALTGLRYIDVVDPAERYSKADLVTDVTTAMTKPSFDVTELFNGLQPVIATLSPDDLNT
                     FTANAATYLSGDGGGLAPMLESIHKLTRFVADREQVIATLMRNLKDISDTMGGHGKDL
                     IQIVDWLNKGPVDGVLGILDEFRKAHLYGEFTDAAAQVIANMGFPAGINNGDYFVYGP
                     ESNKNATDVDDALDRALTVFDDYTDAFKLVPVMWENIPPPPENGAPLECSRGRAQLPE
                     QMDVLLNGRKVVLCNR"
     gene            1452..2411
                     /locus_tag="VGP27_22775"
     CDS             1452..2411
                     /locus_tag="VGP27_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854155.1"
                     /translation="MKVLRNPTAWGAGALALMTIIALVTAMVYVSPPGQKTITFYTDD
                     AAAIRPGDPVRIAGINVGKVKDLALESNQVRVQARVDSSAFVGDRSQVQVRMLTVVGG
                     YYVSISPIGDTPLDAQPIPLERVTMPYSLMRTLADATKITDNVAPKPINESLNQVQQG
                     LTGTNVDSVSAVIDAGNKVMSTIENQRGQVSAILKLSNEYIGDVNGYKEELKDLVRKI
                     SIIEQSLTLYSKGLAGAMTGFGNILDGLSPIFIFYQNHRDEALAKIRDWLEKARMWSE
                     RNGVIVRSLHLARTKIERVLGAQQAAPELLATDLCMPIPGSPC"
     gene            2381..3505
                     /locus_tag="VGP27_22780"
     CDS             2381..3505
                     /locus_tag="VGP27_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016890074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854156.1"
                     /translation="MYANTGKSVLMAASRWPHLFRIAAIALTAAAVVTLSTSCGGPQK
                     SQPNAEYCAVMPDSIGLYVGNPVTQMGYQIGKVTSITPGTTDVRVNFSVTERRLLPVD
                     VKAIVRSTSILADRSLELVGNSASGPRLRAGECIPLNRSWTPKSLSEVIGSATTFVNG
                     INPAESTNVGDVVRQVDQALHGNGAGVNQLLTTSSAVVDSPDQTISDIGSVITNLAEL
                     TTAVTEIRGPLKEALLNAEQNMSDVAVTLDGGNHMVGGFIGAVKAVVDVEENLGGEFQ
                     FTLDATSVALRKLSAHAPWLANLLNPVPWWVNTLANHFNNREFNIGYRPPLYRIRTPD
                     GLALCGFMNASTPGSCADVAGQPYAVDVALLQYVLTQASR"
     gene            3502..4503
                     /locus_tag="VGP27_22785"
     CDS             3502..4503
                     /locus_tag="VGP27_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005073656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854157.1"
                     /translation="MSRRMWRLPVAVATVVTVSSCASLNVSSLPAPGSSYRGGYDIVI
                     EFANALNLPDSAKVVLDGTTVGVVAKVALSTDAVDVTSTIDRNVAVPSNIRAVLQQDT
                     VLGDIYVALERPQTDEAAAPPLSPGGRIPLSQTTSPPQLEDTIAHMANFVASGSIQRI
                     QNTIIGINRIQPPGGEDGIRKMASQVAADLGDLSDNMNLVDLWLNGVSETVDVAHRRI
                     PVLQGMFSEQGMTAFRRFLEIFSYVGTILPSTGSVYAGGFWLVPLLHSLRDALGATQQ
                     SKRTFEAEAPAWRRLLNDFFLPEDKYPAINITSIIGPDGREISGNVQDVLRILGATP"
     gene            4500..5453
                     /locus_tag="VGP27_22790"
     CDS             4500..5453
                     /locus_tag="VGP27_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854158.1"
                     /translation="MRGRDVLSFMAFAVMIVFALSYIGSLGVRVRPPSDRTNLSMTVA
                     DINGIVVDSNILLRGVPVGKVSHIESSVAGATIDFYVDGRFPIPADCDVRLDNLSALG
                     ETYIGLFPRRQGGPMLHDGQHIATELVEQPPSITELAASVTRLLNQLDPDALERIIGE
                     ADAALPDPNSVLPNLGHASELLRNTAANMHGQGRVLLSNFETLLQNAGWVGPVLASTA
                     PELSDSADKAAQVLQGFSVGALEIKPGGMKKFGFFLDRIKKLLDANGGDLKVLGEAFR
                     PRVRAIAGALLNFDPSQILANILATVPEDGAITLRVTIPPN"
     gene            5582..6154
                     /locus_tag="VGP27_22795"
     CDS             5582..6154
                     /locus_tag="VGP27_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854159.1"
                     /translation="MEESASSDEPQGKKPKRVQLSVSLRSVVIAVVITALLAAVGVLA
                     WLYVGARGRLDEQARQSENNVHAEKVALQYAGEAAAMNYQDLNAWKGRLVAGTSPELK
                     DKLSEAATSMEQILVPLQWNSTAHPLAAKVRTKTGGVYVVDAFVSVLTKTAQASEPLQ
                     STATYSVTVDSSKDWQITDVGGVGDALEPK"
     gene            6234..6845
                     /locus_tag="VGP27_22800"
     CDS             6234..6845
                     /locus_tag="VGP27_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854160.1"
                     /translation="MTCVFLPLSGAAVATGPASRADPSDYVRPPEPLPVVIPTASDWV
                     PKFPFPYDQTKGSVTDADINAERDMCQWFNAQYDELTRQINRFQFNRITQNGPGVISG
                     AGSDWDYSIGDLQRQADIVTTNIDQSVDFLAPRAQALTQSTDHSGDVYFPLYQGESFY
                     LLWQHLSNVNAGIKSRQPDWFTGPSVQRALRWGSRIHRSHVCE"
     gene            6980..7537
                     /locus_tag="VGP27_22805"
     CDS             6980..7537
                     /locus_tag="VGP27_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854161.1"
                     /translation="MYHRKSRWKWLAAIASTVGLAFATVAPGHADPPGDVPANPFPEM
                     GHVLAWYDKFEPDQLFIADHPGVWFLSPSGLTCGIWDRGSFGCTGNIPGLPPGSNHIA
                     WFNGNRSVHYGWTAAIQFPPGQPNVTLPPRSYVTYNSTTCAIEPDSTLYCGHGEFKFL
                     ISATQTVFKGYDEPSTRVCLSYGSC"
     gene            7698..8126
                     /locus_tag="VGP27_22810"
     CDS             7698..8126
                     /locus_tag="VGP27_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854162.1"
                     /translation="MCALLVSGGMVVAPSALADSVTRVQDVMATARAGTSCPPLNYNP
                     VVEKAAAIINQSTDDYIDHLSTYQPITDPMPGLKDLGYGGNRAALLQGALSNEADAIK
                     GALLQGHAAIPDCSYTDLGIDVRRNEATDNLLVVYLLAGA"
     gene            8211..>8582
                     /locus_tag="VGP27_22815"
     CDS             8211..>8582
                     /locus_tag="VGP27_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854163.1"
                     /translation="MIRNRLMMISLITGISAISLAPSAYAQDIVTYEVVSNDVAEATV
                     EYFDSSERKVLAAVPLPWRASVTVINARDPSTDGAEVRADWRPRVCDGRAWCPEARPN
                     NWVTVRIYLHGKVICESTLDVG"
BASE COUNT         1837 a         2527 c         2528 g         1690 t
ORIGIN      
        1 atttccctcg tttgccgacg cgatgatcga cgccggctac tatgccacgc acggtactaa
       61 taagctcaac aaagggacgt ggaacgcaag cgaagccgaa tatcaaaccg acattctacc
      121 gttcatgcaa tatgtgtcgg atagcctttt cggtgctatc ggcaggctgg agtacacgca
      181 tatcgacgat ctcacgccga tggtcgacag cgtgcgagcg ctgactgatg tggtgccgcc
      241 attgatgagg ccccaaggca tgtcggagat gctcgtggaa ctgcggtcca ggctcgagaa
      301 aatgtacggg ggtacccccg agcaacgtgc gcttcaagtt cgaatagttc tcgacagcct
      361 tccgggtgtg gcggcgccac ttggtgctgt ggggggtgcc ggatgaagcc tcgcgcggcc
      421 ctgtggcgac tcgcgatcag cggaatagtc gcgatcgccc tgttcatagc gttggcgaac
      481 gtcatcaagc agcccacggc agcagaaaca cgatcgtata cagccgagtt cactgacgca
      541 tcgggattgc accccgacgc tgacgtgcgg gtccgtggag ttcgcgtcgg caaggttcag
      601 tcggtcgacc tcgagcgaag gaacggtcaa agcatcgccg ttgtcggcct gacgtgtgac
      661 cagcgatatg gcgtggtgtc cggcactcgg cttgccatca aattcgaggc gcttaccggc
      721 ttgcgttata tagatgtcgt agatccggcg gaacggtatt cgaaggcgga tctcgtcacc
      781 gatgtgacga cagcgatgac gaagccgtct ttcgacgtca ccgaattatt caatgggttg
      841 caacccgtga tcgccaccct gagtcctgat gacctgaaca cattcacagc gaatgcggca
      901 acatatctct cgggtgatgg tggcggcttg gcaccgatgc tggaaagtat tcacaagctc
      961 accagattcg tggccgaccg tgagcaagtc atcgcgacgt tgatgcgtaa tctgaaggat
     1021 atttccgaca ccatgggcgg acatggaaaa gatctaatcc agatagtgga ctggcttaac
     1081 aagggcccgg tggatggcgt tcttggaata ctcgacgagt ttcgaaaagc ccacctctac
     1141 ggcgaattca cagatgctgc cgcacaggtg atcgccaaca tggggttccc agccggtatc
     1201 aacaatggcg actacttcgt ctacggccca gagtccaaca agaatgccac cgacgtggac
     1261 gacgccctgg accgtgcact caccgtgttc gacgactaca cggacgcctt caagctagtt
     1321 cccgtcatgt gggaaaacat cccgccgccg cctgaaaacg gtgcacccct ggaatgttca
     1381 cgggggcgcg cccaactgcc ggagcaaatg gacgtactcc taaatggccg aaaagtggtg
     1441 ttgtgcaacc gatgaaggtg ttacgcaatc cgactgcgtg gggcgccggc gcccttgcac
     1501 tcatgaccat cattgcgctg gtgaccgcga tggtctacgt cagcccgccg gggcagaaga
     1561 cgatcacctt ctataccgac gacgccgcgg cgattcgccc aggagaccca gtacgaatcg
     1621 ccggaatcaa cgtcggcaag gtgaaggatc tggccttgga atcgaaccaa gtgcgggtcc
     1681 aggcacgggt cgacagtagt gcatttgtcg gtgaccgctc gcaggtgcaa gtccgcatgc
     1741 tgaccgtggt tggcggctat tacgtaagca tctcgccaat aggtgacacc cctttggacg
     1801 ctcagcccat tccgttggaa cgggtgacga tgccttacag tttgatgcgg accctggccg
     1861 acgcgacaaa gatcaccgat aatgtcgccc caaaaccaat caatgaatcg ctcaatcagg
     1921 tgcagcaggg acttaccggc accaatgtgg attcggtgtc ggcggttatc gatgcgggca
     1981 acaaggtcat gtcgacgatt gagaaccaac gcggccaagt gagtgccatc ttgaaactct
     2041 cgaatgaata tatcggggat gtgaatggct acaaagaaga gctcaaagac ttggtccgca
     2101 agatctcgat cattgagcag tcacttactt tgtatagcaa gggcctcgcc ggggccatga
     2161 caggcttcgg caatattttg gatggtcttt caccaatctt catcttttac caaaatcatc
     2221 gcgacgaggc tctcgccaag atccgtgact ggttggagaa agcacgaatg tggtcggaac
     2281 gaaacggtgt aatagtgcgg tccctgcacc tggcgcggac aaagatcgaa cgggtgctgg
     2341 gcgcccagca ggcggcacca gagctattgg ccaccgacct atgtatgcca ataccgggaa
     2401 gtccgtgctg atggccgcgt cgagatggcc acaccttttc cggatcgccg caatcgcgct
     2461 cacagcagcg gcggtggtga cgctgagtac ctcgtgcggg gggccgcaga aaagtcagcc
     2521 aaacgccgaa tattgcgcgg tcatgcccga cagcatcggt ctctacgtcg gtaatccggt
     2581 cacgcagatg ggctaccaga tcggcaaggt cacgagcatc acccccggca cgaccgacgt
     2641 ccgtgtcaac ttcagcgtca ccgagcggcg tctattgccc gttgatgtca aggcaatcgt
     2701 caggtcgaca tcgatccttg ctgaccgatc actggaactt gtcggtaact ctgcttcggg
     2761 gcctcgcctc cgtgcggggg aatgcattcc gctcaaccgt tcatggaccc cgaagagtct
     2821 ctcggaagtg attggttcgg cgactacctt cgtcaacgga atcaatccgg ccgagtcgac
     2881 gaatgtcggc gacgtcgtgc gtcaggtcga tcaagcgttg cacggcaatg gagccggcgt
     2941 aaaccaactg ttgacgacgt catcggcggt agtggatagc ccggatcaaa cgatcagcga
     3001 catcggatcc gtgatcacca accttgccga actgactaca gcggtcaccg agatcagggg
     3061 accgctgaag gaggcattgc tcaacgccga gcagaacatg tctgacgtcg ccgtgacctt
     3121 ggatggtggc aaccatatgg tcggcggatt catcggggct gtgaaagcag tagttgacgt
     3181 ggaagagaac ctcggtggtg aatttcagtt cacgctcgac gcgacgtcgg ttgcgctgag
     3241 gaagctgagt gcacacgcgc cgtggctggc aaacctcctg aaccccgtcc cgtggtgggt
     3301 caatacgctg gccaatcact tcaacaaccg tgaattcaac atcggttacc gtcccccgct
     3361 gtatcgcatc cgtacccccg atgggcttgc gctgtgcggg ttcatgaacg cttcgacgcc
     3421 aggaagctgc gccgatgttg cggggcagcc gtacgcggtc gatgttgcgc tgctgcagta
     3481 cgtcctcaca caggcgagcc gatgagccgc cggatgtggc ggttgcccgt ggccgtcgcc
     3541 accgtcgtga cagtctcctc gtgtgcatcg ctcaacgtga gctcgttgcc tgcgccgggg
     3601 agtagttatc gcggtggata tgacatcgtc atcgaattcg ccaacgcgct caatctgccg
     3661 gatagcgcaa aggtagtttt ggacggcacc acggtcggag tcgtcgccaa ggtggcactc
     3721 tccaccgatg cggtcgacgt cacgtcgaca atcgaccgca acgtcgcggt gccgtcgaac
     3781 atccgtgcgg ttctgcagca ggacacggtc ttgggcgaca tctatgtcgc cctcgagcga
     3841 ccgcaaaccg atgaggcagc ggcacccccg ctgagcccgg gcgggcgcat ccccttgtcg
     3901 cagaccactt ccccgccaca acttgaagac acgatcgcgc acatggcgaa cttcgtggcg
     3961 agcgggtcca tccagcgcat acagaacacg atcatcggta tcaaccgaat ccaaccgccg
     4021 ggaggcgaag atgggatccg caaaatggct tcccaagtgg cagccgacct cggtgacctc
     4081 tcggacaaca tgaatctggt cgacctatgg ctcaacggcg tgtccgaaac ggtcgatgtc
     4141 gcgcacagaa ggatcccggt gttgcaggga atgttctcag agcaaggcat gaccgctttt
     4201 cgtcggttct tggagatctt ttcatatgtt gggacaatcc tgccgtctac cggcagtgtc
     4261 tacgccggtg gtttctggct ggttccccta ctgcattcct tgcgagatgc gctgggcgct
     4321 acccagcaat ccaagaggac gttcgaagcg gaggcgcccg catggcgccg gctactcaat
     4381 gatttcttcc ttccggaaga caaatatccg gcgatcaaca tcacgtcgat aatcggaccc
     4441 gatggtcggg aaatatcggg caacgtgcag gacgtgctgc ggatcttggg tgcgacacca
     4501 tgaggggccg agacgtcttg tcgttcatgg catttgccgt gatgatcgtg tttgcgctga
     4561 gctacatcgg ctcgctcggt gtccgggtca ggccgccctc tgaccgaacc aatctctcca
     4621 tgacggtggc agacatcaat ggcatcgtcg tcgactcgaa catcctgctg cgcggcgtgc
     4681 cggtcggcaa agtcagccat atcgagtcat ccgtggccgg agccaccatc gacttctacg
     4741 tggacggccg cttccccatc cccgccgact gtgatgtgcg gctcgacaac ctatctgcgc
     4801 tcggtgagac ctatatcggc ctgtttccgc ggcgtcaggg cggtccaatg ttgcacgacg
     4861 gccagcacat cgccaccgag ttggtagagc aaccaccatc gatcacggag ctcgcggcaa
     4921 gtgtcacgcg gttgctcaat cagctcgacc ccgatgcgct cgaacgcatc atcggtgagg
     4981 cagatgccgc actgccagat ccaaattcgg tgctacccaa cctgggtcac gctagcgagc
     5041 tgctcagaaa caccgcagcg aatatgcacg gccaaggccg agtgctgctg agcaacttcg
     5101 agacactgtt gcagaacgct gggtgggtcg ggcccgtgct cgcttcgacg gcacccgaac
     5161 tctcggatag cgccgacaag gctgcacagg tcctgcaggg tttttcggta ggcgccctcg
     5221 agatcaagcc gggcggcatg aagaaattcg gattcttcct cgatcggatc aagaagctat
     5281 tggatgccaa cggcggcgac ctcaaagtgc tgggcgaagc atttcggcca cgcgtgcgag
     5341 ccatcgccgg cgcgctgttg aacttcgacc ccagtcagat cctggccaac atcctggcca
     5401 cggtgcccga ggacggcgcc attacgctgc gcgtcaccat ccccccgaac taactaggca
     5461 ggtatggccg cccgaaacca cgaattgagg aaagtacgtg attgtcgata gcaaacaaga
     5521 cgctggacca gatgccgccg tgaccgattc aagcgggtcg gcgtcagagg acgaaacccc
     5581 cattgaggag tcggcgtcaa gcgacgaacc tcaaggcaag aagcccaaaa gagtgcagct
     5641 ttcggtttct cttcgcagcg tggtgatcgc cgtggtgatc accgcccttc tcgccgccgt
     5701 tggcgtgctg gcctggctct atgtcggagc gcgaggccgg ctcgacgagc aggcccgtca
     5761 atccgaaaac aatgtgcacg ccgagaaggt ggctctgcag tacgcgggag aagcggcggc
     5821 gatgaactac caagatctca acgcctggaa gggcaggctc gtggcaggca ccagccccga
     5881 actgaaagac aagttgtccg aggcggccac gtcgatggaa cagatcttgg tacccctcca
     5941 atggaattcg acggcacatc cgctggcggc aaaggttcgc accaaaaccg gcggagtcta
     6001 tgtcgtcgac gctttcgtca gcgtccttac caagaccgcc caagcttcgg agccactgca
     6061 gtcgacggca acatacagcg tcacggtcga cagtagtaag gattggcaga tcaccgatgt
     6121 tggcggagtt ggcgacgcgc tagaaccaaa atagacctct cgaaaggtga ctcatgatgg
     6181 gtcgaaatag gtggctcgcg gtgcgaattc ccttgcgccg ctgcgtcatc gcgctgacat
     6241 gcgttttcct cccgttgtcc ggagcggccg tcgcgacggg gcccgcctcg cgagccgatc
     6301 catccgatta cgtgcggccc cccgaaccat tacctgtggt cattccaacg gcttcggatt
     6361 gggtgccgaa gtttccattc ccttatgacc agaccaaagg cagcgtgacc gatgcggaca
     6421 tcaacgcgga acgggacatg tgccagtggt tcaatgcgca atatgacgaa ttgacgcgcc
     6481 agatcaacag gttccaattc aaccggatca cacagaacgg acctggcgtc atctcgggtg
     6541 cgggttccga ctgggactac agcatcggcg accttcaacg gcaagccgac atcgtcacca
     6601 caaatatcga ccaatcggtg gacttcctcg ccccccgcgc ccaagcgctc actcagtcca
     6661 ctgaccattc gggtgacgtc tactttccgc tctatcaagg tgagagcttc tacttgctgt
     6721 ggcagcactt gtccaacgtc aacgccggga tcaagagccg ccaacccgac tggttcacag
     6781 gcccatcggt gcagagagca ttgcgttggg gtagcagaat tcaccgttcg cacgtatgcg
     6841 agtagaggac gcagtccgaa tacctgacag caggcggcga ccgtgcgact cgtctcgatt
     6901 ctcgagtgac gacgtcgcga agttatcggc atcgacctgg cgcgccggct cgtgggctgc
     6961 tcagaggagg aggatcggaa tgtatcaccg taagagtcgc tggaaatggt tggcggccat
     7021 tgcttcgacc gtcggactgg cgttcgccac cgtggccccg ggacacgcgg acccgcctgg
     7081 tgacgttccg gcgaatccgt ttccagagat gggtcatgtg ctcgcgtggt atgacaaatt
     7141 cgagccagac cagctgttca ttgctgacca tcctggtgtc tggttcttgt caccctccgg
     7201 actcacctgc ggcatctggg acaggggaag ctttggttgc accggcaata tcccagggct
     7261 gccgccgggg agcaatcaca tcgcttggtt caacggaaat cgctccgtgc actacggctg
     7321 gaccgcggcc attcagtttc ccccgggcca acctaatgtg accctcccgc cgcgcagcta
     7381 cgtcacctac aactcaacga cttgtgcgat agaaccagac agcacccttt attgcgggca
     7441 cggcgagttc aagttcttga tttccgcgac acagaccgta ttcaaaggct acgacgagcc
     7501 ctccacgcgt gtgtgcctga gctacgggtc ttgttgacgg aaactctctt gctaccgatg
     7561 agtccggccg gcatcaaacc gcacgacgag atgtcttaga gaaggagttg taagtgtcaa
     7621 ggcaatcacc tgcttcaccc gcaggtcgca gatcgaggtc gatactttcc tggcggaggt
     7681 cgatccgtcc ggtagccgtg tgcgcgctgc tggtttccgg tggcatggtc gtggcaccgt
     7741 cagccctcgc cgattcggta acccgcgtac aggatgtgat ggcgaccgcc cgcgccggaa
     7801 cgtcatgccc gccgttgaac tacaacccag tagtcgagaa ggcagccgcg atcatcaatc
     7861 agtcgaccga tgattacatc gaccatctct cgacatacca accgatcaca gacccaatgc
     7921 ccgggctaaa ggatctcggc tacggcggca atagggcagc cttgcttcaa ggcgccctga
     7981 gcaacgaagc tgatgcgata aagggcgcac tgctacaagg ccatgccgca atccctgatt
     8041 gctcctatac ggacttggga atagacgtac gacgaaacga ggccaccgat aatctcttgg
     8101 ttgtgtacct gttagcgggg gcttagcaat tccggattgc agcggtgaca tcgataggct
     8161 tttaaatggc tcggcgcatt cataccaccc aataccaacg aggggctcat atgattcgta
     8221 accggctcat gatgatttcg ttgattaccg gcatttccgc gatctcgttg gccccgtccg
     8281 cttacgccca ggatatcgtt acatacgagg tggtctcgaa cgacgtcgcc gaagcaactg
     8341 tcgagtactt cgactcctct gagcgaaaag tgttggctgc ggtgccgctg ccatggcgag
     8401 ccagcgtgac ggtgatcaac gctcgcgatc cgtcaaccga cggcgcggaa gttcgcgccg
     8461 actggcggcc ccgcgtgtgt gatggacggg cctggtgtcc agaggccagg ccgaataact
     8521 gggtaacggt ccggatttat cttcacggga aggtcatctg cgaaagcacc cttgatgtgg
     8581 gc
//
LOCUS       DASYLX010000978         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1268468,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000978 DASYLX010000000
VERSION     DASYLX010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268468"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGP27_22820"
     CDS             <1..192
                     /locus_tag="VGP27_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEV7854164.1"
                     /translation="PRGKRQFRRGVLDADKGEVSSYGPPASHHLRWLASANCLLEIEE
                     DVAELAAGSPVQVWDLSSG"
     gene            215..916
                     /locus_tag="VGP27_22825"
     CDS             215..916
                     /locus_tag="VGP27_22825"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEV7854165.1"
                     /translation="MARRPDLRTGPARLVALARTTIPPMHPAGLPFVGAGLALAAAGR
                     RYRWLRNAGLLAAGANAAFFRHPPRVPPARPGVVVAPADGLICLVEDVVPPAELNLPD
                     RPLPRISIFLSIFDAHVQRIPISGEVVSVVHRRGLYKSAELAAASEDNERNSVRIRTP
                     EGHEVVAVQIAGLVARRIVCDIKPGDKVTIGDTYGLIRYGSRLDTYLPEGTEVLVLPG
                     QRAVGGETILAELPR"
     gene            913..1779
                     /gene="pssA"
                     /locus_tag="VGP27_22830"
     CDS             913..1779
                     /gene="pssA"
                     /locus_tag="VGP27_22830"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEV7854166.1"
                     /translation="MKPRVKRPNVSLRMLPSAMTVAAICLGLSAVKFALDDRPTEAMA
                     FLAVAAILDALDGRIARLLKATSKMGEEIDSLADAVNFGVAPAFIVYGTLLSHSRIGW
                     IVVLLYAVCIVLRLARYNTMLAEDQPPYTKEFFVGMPAPAGAIGAIGPLAAKMQFGDG
                     WWTSEPAVVIWMVGVSLLVVSTIPMRKVHTFAISQNMVVPLLAALAILVAASILYGYI
                     VILLIILGYVIHIPFAVRTEAWLSEHPEVWGDKPKEQRQARRAVRRVRRAQPRTHRRS
                     MARLGLRRPPGP"
     gene            1781..>2367
                     /locus_tag="VGP27_22835"
     CDS             1781..>2367
                     /locus_tag="VGP27_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV7854167.1"
                     /translation="MTGFDVADRPVPRQQLTLTARLNTSALDSRRGVVRLHPEAIAAL
                     GIREWDAVALMGSRTTAAVAGVAPDGTPAGTALLDDVTLSNAGLRENTTVLVAPVTVY
                     GARSVTVRGSNLATRSIPSATLRQALLGKVMTVGDTVSLLPRELGPDISTTAASSALA
                     SAVGITWTSELLTVTGVDPAGPVSVQPNSVVKWGDG"
BASE COUNT          349 a          827 c          822 g          369 t
ORIGIN      
        1 ccacgcggca agcggcagtt ccgccgcggt gtgctggacg ccgacaaggg cgaggtctcc
       61 agttacgggc cgcccgcgtc gcaccatctg cgctggctgg catcggcgaa ctgcctgctg
      121 gagatcgagg aggacgttgc cgagctggcg gcgggatccc ctgtccaggt ttgggacctc
      181 tcgagcgggt agccacccgt agaatcgcca cacgatggcc agacgccccg acctcagaac
      241 aggccccgcg cgactggtgg ccctggcgag aaccacgatt cccccgatgc atccggcagg
      301 cctaccgttc gtcggcgcag gcctggcgct cgccgcggca ggacgccgct atcggtggct
      361 gcgcaacgca ggcctgctgg ccgcgggcgc caacgctgcg ttcttccgcc acccgccccg
      421 agtgccgccc gcccgccccg gcgtggtcgt cgcgcccgcc gacgggctga tctgcctcgt
      481 cgaggacgtg gtgccgcccg ccgaactgaa cctgccagac cgtccgctgc cccggatcag
      541 catcttcctg tcgatcttcg acgctcacgt ccagcgcatc ccgataagcg gtgaggtggt
      601 gtcggtcgtg caccgtcggg ggctgtacaa atccgccgag ctcgccgcgg ccagcgagga
      661 caacgaacgc aacagcgtgc gcatccgcac gcccgagggc cacgaggtgg tggccgtcca
      721 gatcgcgggt ctggtcgcgc gccgcatcgt ctgcgacatc aagcccggcg acaaggtgac
      781 gatcggcgac acctacggcc tcatcaggta cgggtcgcgg ctggacacct acctgccgga
      841 gggcaccgag gtgctcgtgc tgccgggcca acgggcggtc ggcggcgaga cgatccttgc
      901 ggagctgccg cgatgaagcc ccgcgtcaag aggcctaacg tcagcctgcg catgctgccg
      961 agcgcgatga cggttgcagc gatctgcctc ggcctttcgg cggtgaaatt cgcgctggac
     1021 gaccgcccga ccgaggcgat ggcgtttctg gcggtggccg cgatcctcga cgcactcgac
     1081 ggtcgcatcg cgcggctgct caaggccacg tcgaagatgg gcgaggagat cgactcactc
     1141 gccgatgcgg tgaacttcgg tgtcgcaccg gcattcatcg tctacggcac gctgctctcg
     1201 cactcgcgca tcggctggat cgtcgtgctc ctctacgcgg tgtgcatcgt gctgcggctg
     1261 gcgcggtaca acacgatgct ggccgaggac caaccgccct acaccaagga gttcttcgtc
     1321 ggcatgcccg caccggcggg cgcgatcggc gcgatcggcc cgctggcggc caaaatgcag
     1381 ttcggcgacg gctggtggac ctcggagccc gccgtggtga tctggatggt cggcgtctcg
     1441 ctgctggtgg tcagcaccat cccgatgcgc aaggtgcaca ccttcgccat ttcccagaac
     1501 atggtggttc cgctgctggc ggcactggcg atcctggtcg ccgcctcgat cctctacggc
     1561 tacatcgtga tcctgctgat catcctgggc tacgtgatcc acatcccgtt cgcggtacgg
     1621 accgaggctt ggctgtcgga acacccggag gtgtggggag acaagccgaa ggagcaacgt
     1681 caggcgcggc gggcggtccg ccgggtgcgc cgggcccagc cgcgcaccca ccgtcgttcg
     1741 atggcccggc tcgggctccg cagaccgccg ggcccctgac atgaccgggt tcgacgtcgc
     1801 ggaccggccg gtgccacggc agcagctcac acttaccgcg cggctgaaca cctcggcgct
     1861 ggactcgcgc cgcggtgtcg tccgccttca ccccgaagcc attgcggcgc tgggaatccg
     1921 ggaatgggat gcggttgcgc tgatgggttc gcggaccacc gccgcggtcg cgggtgtggc
     1981 accggacgga accccggcgg gcacggcgct gctcgacgac gtgacgctgt ccaacgccgg
     2041 gctgcgggag aacaccaccg tgctcgtcgc accggtgacg gtgtacggcg cgcggtcggt
     2101 gacggtgcgc ggttcgaacc tggccacgcg gtcgattccg tcggcgacac tacgccaggc
     2161 cctgctcggc aaggtgatga cggtcggcga caccgtgtcg ctgctgccgc gcgagctggg
     2221 ccccgacatc tccacgaccg ctgccagcag cgcgctggcg tcggccgtcg gcatcacctg
     2281 gacgtccgaa ctgctgacgg tgacgggcgt ggacccggcc ggacccgtta gcgtgcaacc
     2341 gaattcggtc gtcaaatggg gtgacgg
//
LOCUS       DASYLX010000979         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2255839,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000979 DASYLX010000000
VERSION     DASYLX010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255839"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VGP27_22840"
     CDS             <1..631
                     /locus_tag="VGP27_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV7854168.1"
                     /translation="EYCALTDHSPRLRIANGLSPERLRKQLEVIDELREAVAPLRILT
                     GIEVDILEDGSLDQEPELLERLDVVVASVHSKLAMDKPAMTRRMLRAVENPHADVLGH
                     CTGRLVTGGRGMRPESKFDAEKVFTACRDNGTAVEINSRPERRDPPTRLLDLALEIGC
                     LFSIDTDSHAPGQLEFLGYGAQRALEAKVPVDRIVNTWTAEKLLDWTAS"
     gene            complement(657..1907)
                     /gene="dinB"
                     /locus_tag="VGP27_22845"
     CDS             complement(657..1907)
                     /gene="dinB"
                     /locus_tag="VGP27_22845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV7854169.1"
                     /translation="MFVSGVRLDTPAQSATILHADLDSFYASVEQRDDPALRGRPVIV
                     GAGVVLAASYEAKAYGVRTAMGGRQARQLCPHALVVPPRMQAYSEASDAVFDVFHDTS
                     PVVEPLSVDEAFLDVSGLRRVSGTPVEIAARLRAQVLDRAGLPITVGVARTKFLAKVA
                     SQEAKPDGLLLVPPDRELAFLHPLPVRRLWGVGAKTAEKLHAHGIETVADVAELGQST
                     LATMVGGAMGHQLFALSHNIDRRRVVTGVRRRSVGAQRALGRRGNTMSAAEVDAVVIN
                     LIDRITRRMRTAGRTGRTVVLRLRFDDFGRVTRSHTMPRATASTDVVLEAARDLVAAA
                     APLIAERGLTLVGFAVSNIDRHGAQQLELPFEVGPLACWRHVDTVALDDAVDEVRKRY
                     GNSAVTRGVLVGRDPGLEMPHLPD"
     gene            complement(1931..2575)
                     /locus_tag="VGP27_22850"
     CDS             complement(1931..2575)
                     /locus_tag="VGP27_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854170.1"
                     /translation="MTTASRPRAARGRRAGRPSGDDRELAILETAERLLEERPFAEIS
                     VDDLARGAGISRPTFYFYYPSKDAVLLTLLDRIIAEADDRADAALGPMDTAVDPAGVW
                     RAINALFESFSNHRAVMIAGSAARPTNAEMHSMWSKFMQKWIDYTAHSIQAERDRGAA
                     PVTIPARDLAIALNLMNERTMFAVHAAEKDSVARDRIVDTLAHIWVSSIYGKAP"
     gene            2652..>3358
                     /locus_tag="VGP27_22855"
     CDS             2652..>3358
                     /locus_tag="VGP27_22855"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854171.1"
                     /translation="MTEFVDVVIVGAGISGISAAWHLQNRCPDKSYMILERRENLGGT
                     WDLFKYPGLRSDSDMYTLGFRFKPYTDEQAIAEGPSILNYLKEAAAENGIDKHMRFGQ
                     TVVAADWSDADNRWTLRIESAGEQREISCAFLFGCTGYYNYDEGYSPDFPGAEDFDGT
                     IVHPQHWPEDIDYQGKKIVVIGSGATAVTLIPALIRSGSGHVTMLQRTPTYIAGMPDT
                     DPFAAKATKLLPEKAAYT"
BASE COUNT          591 a         1094 c         1141 g          532 t
ORIGIN      
        1 cgagtactgt gcactgaccg accactcgcc gcgcctgcgg atcgccaacg ggctgtcgcc
       61 ggagcggctg cgcaagcagc tcgaggtgat cgacgaactg cgcgaagccg ttgcgccgct
      121 gcgcatcctc accggtatcg aagtcgacat cctggaggac gggtcactcg atcaggagcc
      181 cgagttgctc gagcgactcg acgtcgtggt ggccagcgtg cactcgaagc tggcgatgga
      241 caagcccgcg atgacgcggc ggatgctcag ggccgtggag aacccgcacg ccgacgtcct
      301 cggccactgc acgggccgac tggtgacggg cggccgcggg atgcggccgg agtcgaagtt
      361 cgacgcggag aaggtgttca ccgcatgccg cgacaacggc acggcggtgg agatcaattc
      421 gcggccggag cgccgcgacc caccgaccag gttattggac ctggcgctgg agatcggatg
      481 cctgttctcg atcgacaccg actcgcatgc gccggggcag ctggaattcc tcggttacgg
      541 cgcgcaacgc gcgctggagg cgaaggtgcc agtggaccga atcgtgaaca cgtggacagc
      601 cgagaagctg ctcgactgga cggcctcgta acgtagccgt ggaccgctgc gcttgttcag
      661 tcgggcaggt gcggcatctc cagaccaggg tcgcggccga cgaggacgcc gcgcgtgacc
      721 gccgagttgc cgtagcgctt gcggacttca tcgacggcgt catcgagtgc cacggtgtct
      781 acgtgtcgcc agcacgcgag tgggcccact tcgaacggca actccaactg ctgcgcgccg
      841 tggcggtcga tgttggagac tgcaaaaccg accagcgtca agccccgctc ggcgatcaac
      901 ggggcggccg ccgctacgag gtctcgggca gcttcgagaa cgacgtcggt cgatgccgtt
      961 gcgcgcggca tggtgtgcga acgggtgacg cggccgaagt cgtcgaaccg cagccgcagc
     1021 accacggttc ggcccgtccg ccctgccgtg cgcattcgtc gggtgatcct gtcgatgagg
     1081 ttgatcacga cggcgtcgac ctcggccgcc gacatggtgt tgccgcggcg tccgagagcg
     1141 cgctgcgcgc cgacggagcg tcgccgcaca cccgtcacca cgcggcgtcg atcgatgttg
     1201 tgcgacaacg cgaacagctg atggcccatc gcaccgccga ccatggtggc gagcgtcgac
     1261 tggcccagct cggcgacgtc ggcgacggtc tcgatgccgt gcgcgtgcag tttctccgcg
     1321 gtcttggcgc cgacgcccca cagtcggcgc accggcagcg ggtgcagaaa ggccagctcg
     1381 cggtcgggcg gaacgagcag cagaccgtcg ggtttggctt cctgactggc caccttggcg
     1441 agaaatttgg tgcgcgcgac gccgacggtg atcggcagcc cggcgcggtc gagcacctgg
     1501 gcccgtagcc gggcggcgat ctcgaccggc gtgccggaga cccggcgcag gcccgacacg
     1561 tcgaggaaag cctcgtcgac cgagaggggc tccactaccg gtgaggtgtc atggaagaca
     1621 tcgaacaccg catcgctggc ctccgagtag gcctgcatac gcggcggcac cacgagcgcg
     1681 tgcgggcaca gctgccttgc ctggcgtccc cccatcgcgg tgcgcacgcc gtaggccttc
     1741 gcttcgtaac tcgccgcgag cacgacgccc gcgccgacga tcacgggccg gccgcgcagc
     1801 gcgggatcgt cacgctgttc gaccgatgcg tagaacgagt cgagatcagc gtgcaggatc
     1861 gtcgccgact gggccggcgt atcgagccgc acacctgaca cgaacatatg ttcgcatcag
     1921 tgtccgacag ttacggcgct ttgccgtaga tgctgctcac ccagatgtgc gcgagcgtat
     1981 cgacgatgcg gtcgcgggcc accgaatcct tctcggcggc atggactgcg aacatcgtgc
     2041 gttcgttcat cagattgagt gcaatcgcga gatccctggc gggaatggtc acgggcgccg
     2101 caccgcggtc gcgttcggct tggatggaat gcgccgtgta gtcgatccac ttctgcatga
     2161 acttcgacca catcgagtgc atttcggcgt tcgtcggccg agcggccgaa cccgcgatca
     2221 tgacggcccg gtggttgctg aacgactcga acagggcgtt gatggcgcgc cagacgccgg
     2281 ccggatcgac tgctgtatcc atcgggccga gggcggcgtc ggcccgatcg tcggcctcgg
     2341 cgatgatccg atcgaggagc gtcagcagta ccgcgtcctt ggacgggtaa tagaagtaga
     2401 aggtcggccg tgagatcccg gctcccctgg ccagatcgtc aaccgaaatc tcggcaaacg
     2461 ggcgctcctc gaggagacgc tctgcggttt cgagaattgc cagctcacgg tcgtcgccgg
     2521 acggccgacc ggcacggcgg ccgcgggccg cgcggggtcg gctggcggtt gtcacccccg
     2581 tcactttaca cagcgtcgag agtgtcaaca tactgttgac taattcgaca cggcgttgat
     2641 agctttacac catgactgaa tttgtcgacg tcgtcatcgt cggggcgggc atctcgggaa
     2701 tcagcgcggc ctggcatctg cagaaccgct gccccgacaa gagctacatg atcctggagc
     2761 gccgcgagaa cctcgggggc acctgggatc tgttcaagta cccgggcctg cgctcggact
     2821 ccgacatgta cacgctgggg tttcgattca agccgtacac cgacgagcag gcgatcgccg
     2881 aggggccttc gatcctcaac tatttgaagg aagccgccgc cgagaacggc atcgacaagc
     2941 acatgcggtt cggccagacc gtggtggccg ccgactggtc cgacgccgac aaccgctgga
     3001 cgctgcgcat cgagagcgcc ggggagcagc gcgagatcag ctgcgcgttc ctgttcgggt
     3061 gcaccggcta ctacaactat gacgagggct actcgcccga ctttccgggc gcggaagact
     3121 tcgacggcac gatcgtgcac ccgcagcact ggccggagga catcgactac caaggcaaga
     3181 agatcgtcgt gatcggcagc ggcgcgaccg ccgtgacgct gatcccagct ctcatcaggt
     3241 cggggtccgg ccacgtgacc atgctgcagc gcacgccgac gtacatcgcc gggatgcccg
     3301 acaccgatcc gttcgctgcg aaagccacca agctactacc ggaaaaggcg gcctacac
//
LOCUS       DASYLX010000980         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_611684,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000980 DASYLX010000000
VERSION     DASYLX010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611684"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGP27_22860"
     CDS             <1..180
                     /locus_tag="VGP27_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEV7854172.1"
                     /translation="LRLGMHVGIAYGVWTACGVAVVAVAAKVLFDEPLTWLMGLGIAL
                     IVGGVLTIEMAGAAG"
     gene            complement(152..1354)
                     /locus_tag="VGP27_22865"
     CDS             complement(152..1354)
                     /locus_tag="VGP27_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854173.1"
                     /translation="MALGRIDDVDVAARDRRARVAVAALFLTNGALFANLLPRYPEIK
                     ADLALSNAHYGIAIAAFSGGALVAGLAAGALIRRFRSARVAVAGSIGIAVFVFVAALA
                     PSAVALASALFVAGASDAITDVAQNAHALRLQRIYGRSIINSSHAVWSAGAVLGGAMG
                     AAAIALHIPRTTHLAVSALLFSAVVLAAYPFLLRGPDEEDHPSSRTERAGRPGLAVYV
                     VLLALVGIAISGAVVEDAGSSWAAVYLRDSLGTPGAIAVSGYIALVGFQFVGRMIGDR
                     LVDRFGERAVARVGGLIAAAGMGAALAFPGVVATIAGFGAAGFGLATVIPAAMHAADQ
                     LPGLRPGSGLTVVTWLLRIGTISSAPIVGLVADATSLRVGLLVVPIAGLSVMILAVAL
                     SPRRRPSR"
     gene            1353..2741
                     /gene="lysA"
                     /locus_tag="VGP27_22870"
     CDS             1353..2741
                     /gene="lysA"
                     /locus_tag="VGP27_22870"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV7854174.1"
                     /translation="MVTDVTDESPTTASAEWDAEAMRSEATAEELINLFPPGTRTDGD
                     GTLVVGGCRLDDVAAAFGTPAIVVAEDALRNRARMYLGAFRSRWPRSDLAFASKSFPC
                     TAVQRVMVEEGLHLDVAGGGEILTAIKAGVDPDRLVLHGNAKTDEELGLAVKHGLGLV
                     VVDNFDDIDRLERIVPHGSRQRCLVRVIPGVEAATHASQATGHAGSKFGMLPDDARDA
                     IARIERSSVLQLDGVHTHVGSQLLNVEQLAAAVEPIAKLGTFETYDLGGGLGVRYTYD
                     EHPPSVDDYAEAMVAQARALLPDGCRIIVEPGRSMVATSACTLYRVTTVKRGAITHVA
                     VDGGMGDNLEVSLTGQRFEAALVDRVGGDGETVTVVGRHCESGDQLVDGIALRDPAVN
                     DLLAVPVTGAYCYTMSNQYNGARRVPVVFARDGEARLVVRRDTWGDLLSRDVDADARS
                     TTWDGGGERRAR"
     gene            2747..>3864
                     /locus_tag="VGP27_22875"
     CDS             2747..>3864
                     /locus_tag="VGP27_22875"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV7854175.1"
                     /translation="MVETPPSLGELARRYGVATEYQDWTGRHVDVPDTTLVAVLAALG
                     VSATTEDERAAALAAHDRDHWSRSLPPTIVARAETESSFWVHVTHGSPVGLWIRLEDG
                     SVRAGLRQLENHTPPYDLGDRLVGEATFELPGDLPLGYHRLYIQAGSSESSTPLIVVP
                     AKLELPPRLRAKRTWGLATQLYSVQSERPWGVGDLTDLADLAVWSGAQHGAGYILVNP
                     LHAAAPVAPMEPSPYLPTSRRYFNPLYLRPEVIIEYAEVRGRGRIRKARADLRHRARR
                     EELIDRDAAWKAKRAALQTVYSVQRSAGRELAYAAYREREGRSLEDFAVWCALAEKHG
                     PDWHQWPDELAHPASARVAEFAVKHADAVDFHRWLQWQL"
BASE COUNT          638 a         1356 c         1326 g          544 t
ORIGIN      
        1 ctgcggttgg gcatgcacgt cggcatcgcc tacggcgtct ggaccgcgtg cggcgtggcg
       61 gtggttgccg tcgccgccaa ggtcctcttc gacgagccgc tgacctggtt gatggggctg
      121 ggtatcgcgt tgatcgtggg aggcgtgctg actatcgaga tggccggcgc cgcgggctga
      181 gcgcgaccgc caggatcatc accgacagcc ccgcgatcgg gacgaccagc agccccaccc
      241 gcaagctggt cgcgtcggcg acgagaccga cgatcggggc cgacgatatg gtgccgatcc
      301 gcagcaacca cgtcaccacc gtcagcccgg atcccggacg gagtcctggc agctgatctg
      361 ccgcatgcat cgcggccggt atcaccgtcg cgaggccgaa accggcggcg ccgaaccctg
      421 cgatcgtggc gacaacgcca gggaacgcca acgccgcacc catgcccgcc gctgcgatga
      481 gtccgccgac tctggccacg gcccgttcac caaaccggtc caccaggcgg tcgccgatca
      541 tgcgcccgac gaattggaag ccgaccagag cgatatagcc cgataccgcg atcgcgccgg
      601 gggtgccgag gctgtcgcgt aaatacactg ccgcccaaga actgccggcg tcctccacga
      661 ccgctcctga gatcgcgatg ccgaccagcg caagcagtac cacgtagacc gccaacccgg
      721 gtctccccgc cctttcggtt cgcgaagacg ggtgatcctc ctcgtccggc ccgcgcagca
      781 ggaacgggta ggcggcgagc actaccgcgc tgaacagcaa cgccgatacc gccagatgag
      841 tagtgcgtgg gatgtgcaac gcgatcgcgg ccgcacccat ggcgccgccg agtaccgcac
      901 ccgccgacca caccgcgtgc gaagaattga tgatcgagcg cccgtagatc cgctgcaggc
      961 gcagcgcgtg ggcattctgc gcgacgtcgg tgatcgcgtc ggatgcgccg gcaacgaaga
     1021 gcgccgaggc cagtgcgacg gcggagggcg ccagcgcggc gacgaacacg aacacggcga
     1081 tgccgatgct gcccgcgacg gccacccggg ccgaacggaa gcggcggatg agcgcgcccg
     1141 ccgccaaccc agccaccagc gctccgcctg agaacgccgc gatcgcgatt ccgtagtgcg
     1201 cgttggacag cgcgaggtcg gccttgatct cggggtagcg cggcagcagg ttggcgaaca
     1261 gcgccccgtt ggtgaggaac aacgccgcga ccgccaccct ggcccggcgg tcgcgcgcgg
     1321 cgacgtcgac gtcgtcgatt cgtcctagcg ccatggtcac cgacgttacc gacgagtcgc
     1381 ccacgaccgc gtcggcggag tgggacgctg aggccatgcg cagcgaggcg accgccgagg
     1441 aactgatcaa tctgttcccg ccgggcacgc gaaccgacgg cgacggcacc cttgtggtcg
     1501 gcggctgtcg tctcgatgat gtcgccgccg cgttcggcac ccccgccatc gtcgtcgcag
     1561 aggacgcgct gcggaaccgg gcgcggatgt acctcggcgc gttccgcagc aggtggccgc
     1621 gcagcgatct ggcgttcgcg tcgaagtcgt tcccgtgtac cgcggttcag cgggtgatgg
     1681 tcgaggaggg cctgcacctc gacgtggcgg gcggcggcga gatcctcacc gcgatcaagg
     1741 cgggcgtcga cccggaccgg ctggtgcttc acggcaacgc caagaccgac gaggaactcg
     1801 ggctggccgt caagcacggc ctcgggctcg tcgtcgtcga caacttcgac gacatcgacc
     1861 ggctggagcg catcgtgccg catgggagcc ggcagcgctg cctggtgcgg gtgattcccg
     1921 gtgtcgaggc cgccacgcac gcctcgcagg cgaccgggca tgccggctcc aagttcggca
     1981 tgctgcccga cgatgcccgg gacgcgatcg cccggatcga gcgaagctcg gtgctgcaac
     2041 tcgacggcgt ccacacccat gtcgggtcac agctgctcaa cgtcgagcag ctggccgccg
     2101 ccgtcgagcc gatcgccaaa ctgggcacct tcgagaccta cgacctcggc ggcggcctcg
     2161 gcgtgcgcta cacctacgac gagcacccac ccagcgtcga cgattacgcc gaagcgatgg
     2221 tggcgcaggc ccgcgctctg ctgcccgacg gctgccgcat catcgtcgag ccgggccgca
     2281 gcatggtcgc taccagtgcg tgcacgctgt accgcgtcac gacggtcaag cgcggggcga
     2341 tcacccatgt ggcggtcgac ggcggcatgg gcgacaacct cgaggtctcg ttgacggggc
     2401 agcgcttcga ggcggccctg gtcgacaggg tgggcggcga cggcgagacg gtcaccgtgg
     2461 tcgggcggca ctgcgaatcc ggcgaccagc tcgtcgacgg catcgcgctg cgcgatcccg
     2521 cggtcaacga cctgctcgcg gttccggtca ccggcgccta ctgctacacg atgtccaacc
     2581 agtacaacgg cgctcgccgg gtgcccgtgg tgttcgcccg cgacggcgag gcgcgactgg
     2641 tggtccgccg cgacacctgg ggcgacctat tgagccgcga tgttgatgcg gacgcgagga
     2701 gcacgacatg ggatggagga ggcgaacggc gggcaagata agcgacatgg tcgaaacccc
     2761 gccgtcgcta ggcgaacttg cccgccgcta cggcgttgcc actgaatacc aggactggac
     2821 gggtcgccac gtcgacgtgc cggataccac cttggttgcc gtgctggcgg cgctcggtgt
     2881 ctccgcgacc acggaggacg agcgggcggc ggcactcgcc gcccacgacc gcgaccactg
     2941 gagtcggtcc ctgccgccga ccatcgtcgc ccgcgccgag accgaatcgt cgttctgggt
     3001 gcacgtcact cacgggagcc cggtgggcct gtggattcgg ctggaagacg gcagcgtgcg
     3061 cgccggcctc cgccagctcg aaaatcatac tcccccatac gatctcggcg accggctcgt
     3121 cggcgaggcc acattcgagc tgcccggcga tcttccgctt ggctatcacc ggttgtacat
     3181 ccaggccggc tcatcggaat cgagcacacc gctgatcgtc gtgccggcca agctggagct
     3241 gccaccgcgg ctccgagcca agcgaacctg gggccttgcc actcagctct acagcgtgca
     3301 gtccgaaaga ccctggggcg ttggcgattt gaccgacctc gccgatcttg cagtgtggtc
     3361 cggcgcacag cacggcgcgg gatacatcct ggtcaatccg ctgcacgccg ccgcgccggt
     3421 cgcgccgatg gagccgtcgc cctacctgcc aacctcgcgg cgctacttca atccgctcta
     3481 cctgcggccc gaggtgatca tcgaatacgc cgaggtccgc ggtcgcggcc gcatccgaaa
     3541 ggcacgggcc gacctccgcc atcgcgcgcg gcgcgaggaa ctcatcgatc gcgacgctgc
     3601 gtggaaagcc aagcgtgccg cattgcagac ggtgtactcc gtccagcgat ccgcgggcag
     3661 ggagctggcg tatgcggcgt accgcgaacg ggaggggcga agcctcgagg acttcgcggt
     3721 ctggtgtgcg ctcgccgaga agcacggccc cgactggcac caatggccgg acgaactcgc
     3781 gcatccggcg agcgcccgtg tcgccgaatt cgccgtgaag cacgccgacg cagtcgattt
     3841 ccaccgctgg ctgcagtggc agct
//
LOCUS       DASYLX010000981         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1489273,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000981 DASYLX010000000
VERSION     DASYLX010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489273"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGP27_22880"
     CDS             complement(<1..551)
                     /locus_tag="VGP27_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854176.1"
                     /translation="MAEPLDVAARLAEGRPAVDNIGEYVWACHLLGYQNPDLTLHTAQ
                     VRDWYGSEDGLDLRALDADRAALDAVAAAADSARQLQEQQVGALAGAWQGRGGDASRE
                     FLLRHGAASAAAASAVRDAVDALAVLRDDLWHAVDDKVAGAVTIDDRRQAQRAEWLTA
                     TRTVTTGAGDRAAASELIDQQVKP"
     gene            complement(555..860)
                     /locus_tag="VGP27_22885"
     CDS             complement(555..860)
                     /locus_tag="VGP27_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEV7854177.1"
                     /translation="MGEADIARVDVAAVLGAARQYDAAADILDTAIRTHLTGLRFDGA
                     VAGRIHTAQGDSLRLAIDDVAQRLRQWARAATEIAAELRSCADRYVEVDARGARRIG"
     gene            complement(906..>1697)
                     /locus_tag="VGP27_22890"
     CDS             complement(906..>1697)
                     /locus_tag="VGP27_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV7854178.1"
                     /translation="GAASAAAPRAYRAAGANVIAINAEPNGLNINDGCGSTHMRTLQQ
                     AVVTHGADLGLAHDGDADRCLAVDGDGEIVDGDAIMVILALAMREAGELASNTLVATV
                     MSNLGLHLAMRSAGIEVRTTGVGDRYVVEELRAGEYSLGGEQSGHIVMPALGPTGDGI
                     VTGLRLMSRIAQTRMPLAALASTMQQLPQVLINVEVHDKATVADAPSVRSAVAEAEAE
                     LGDTGRILLRPSGTEQMVRVMVEAADEDTARQLAIRVAESVSEQR"
BASE COUNT          226 a          611 c          583 g          277 t
ORIGIN      
        1 ggcttcacct gctgatcgat cagctcgctc gccgccgcgc ggtcccccgc tccagtggtc
       61 accgttcgcg tggcggtcag ccactcggca cgctgcgcct gacggcggtc gtcgatcgtc
      121 acggcgccag cgaccttgtc gtcgaccgca tgccagagat cgtcgcggag tacggccagt
      181 gcgtcgacgg catcccgcac ggccgatgcc gcggccgcgg atgctgcacc gtgccggaga
      241 aggaactcgc gcgaagcgtc gccaccgcgg ccctgccatg ccccggccaa tgcgccgact
      301 tgctgctcct gcagttgacg ggcgctgtcg gccgccgccg cgaccgcatc caacgccgct
      361 cgatcggcgt ccagcgctcg caagtccagg ccgtcttcgc tgccgtacca gtcccgaacc
      421 tgtgcggtgt gcaaggtgag gtccgggttc tggtagccga gcagatggca tgcccacacg
      481 tattcgccga tgttgtcgac tgccgggcgc ccctcggcca gccgggcggc gacgtcgagc
      541 ggttcggcca tggctcagcc gatccgccgg gcgccgcgtg cgtcgacctc gacgtagcgg
      601 tccgcacacg aacgcagttc ggcggcgatc tctgtggcgg cgcgcgccca ttgccgcagt
      661 cgttgcgcca cgtcgtcgat ggccagtcgc agcgagtcgc cctgtgcggt gtgtatgcgt
      721 cccgcgacgg caccgtcgaa ccggagaccg gtcagatgtg tccgaatggc cgtgtcgagg
      781 atgtcggccg ccgcgtcgta ctggcgtgca gcgccgagca ccgccgcaac atcgacacgg
      841 gctatgtcag cttctcccac actggctacg acggcgccgc aaccctgcgg gttccacccg
      901 atccctcagc gttgctcgct caccgattcg gcgacgcgga tggccagctg gcgtgcggtg
      961 tcttcgtcgg ccgcttcgac catcacccgc accatctgtt cggttccgga gggccgcaac
     1021 aggattcggc cggtgtcacc caactcggcc tcggcctcgg caacggctga gcggaccgaa
     1081 ggcgcgtcgg cgacggttgc cttgtcgtgc acctcgacat tgatcagcac ctggggcagc
     1141 tgttgcatcg tcgacgccag cgcggcaagc ggcatgcgcg tctgcgcgat ccgggacatc
     1201 agccgcagcc cggtgacgat gccgtcgccg gtcgggccca gcgcgggcat cacgatgtgg
     1261 ccggactgtt cgccgccgag cgagtactcg cctgcgcgca gttcctccac gacgtagcgg
     1321 tcgccgacgc cggtggtgcg aacctcgatg cccgccgagc gcatcgccag atgcagcccg
     1381 aggttgctca tcacggtggc gaccagggtg ttggacgcca actcgccggc ctcgcgcatc
     1441 gccagcgcca ggatcaccat gatggcgtcg ccgtcgacga tctcgccgtc gccgtcgacg
     1501 gcaaggcagc ggtcggcgtc gccgtcgtgg gccaggccga ggtcggcgcc gtgcgtcacg
     1561 acggcctgct gcagcgtccg catgtgggtg gatccgcagc cgtcgttgat gttcagcccg
     1621 ttgggctcgg cgttgatggc gatgacgttg gcgcctgcgg ccctgtacgc ccgtggcgcg
     1681 gcggccgaag cggcgcc
//
LOCUS       DASYLX010000982         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1489328,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000982 DASYLX010000000
VERSION     DASYLX010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489328"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VGP27_22895"
     CDS             <1..1218
                     /locus_tag="VGP27_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7854179.1"
                     /translation="AIAPQVMFTDLGLKYVGPIDGHDEHAVEVALRNARGFNAPVIVH
                     VVTRKGMGYGPAEADEAEQMHSTGVIDPQTGLATKVPGPGWTSTFADALISYAAERRD
                     IVALTAAMAGPTGLTAFGKRFPDRLFDVGIAEQHAMTSAAGLAMGGLHPVVAIYSTFL
                     NRAFDQIMMDVALHKLPVTLVLDRSGVTGPDGASHNGMWDLSMLNIVPGMRVAAPRDA
                     ARLYEELGEALQVSDGPTTLRFPKGDVGEDIPALRRRDGIDILAVPADGLSDDVLLVA
                     VGAFASMAKSVAERLRNQGIGVTVIDPRWVLPVPAAIGGLAEAHKLVVTLEDNGVNGG
                     VGSAVSAALRRAEIDVPCRDVGVPQEFQAHASRGEVLADVGLTDQVIARQVTGWVAAL
                     GSAVSEHDVSQLD"
     gene            complement(1202..>2383)
                     /locus_tag="VGP27_22900"
     CDS             complement(1202..>2383)
                     /locus_tag="VGP27_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEV7854180.1"
                     /translation="PPDEQRRVRGRRRSRHGDRPPDEVVSFAEYERLYRHSWGDPEIR
                     WLMSTVPTAMIFDDHDIRDDWNTSASWRAEMAKLPWWRDRIRAGLASYWVYQHLGNMS
                     PDELAGNDDYRRLVSTDGDTWCHLAELAERADREVDCNKGIRFSYRWDLGRTRLIMID
                     SRNGRILESGERMMIGDNEFRWLEAQAEEHPETLDHLLLASSVPWLMPPAIGDLETLN
                     ERAADRTGWRGTLGEKLREAGDFEHWPAFYKSFVRLGEMIARIANHPDGPSTISVLSG
                     DVHHSYAARAEVDGVSPTGATVHQLVCSPVHNYVPLFIKPVFTLSWTRPAAAVTRWWV
                     RRRGVPSPPMTWTDVCGPLFGNTIATVEVNDRTAEVFFEQPRKSTTLEEAARVSISRA
                     G"
BASE COUNT          358 a          814 c          827 g          384 t
ORIGIN      
        1 gcgatagccc cgcaggtgat gttcaccgac ctgggcctca aatacgtcgg accgatcgac
       61 ggccacgacg agcacgctgt cgaggtggcg ctgcgcaacg cccgcggctt caacgcgccg
      121 gtgatcgtgc acgtcgtcac ccgcaagggc atgggctacg gccccgccga ggccgacgag
      181 gccgagcaga tgcactcgac gggcgtcatc gacccgcaga cgggactggc caccaaggtg
      241 ccgggtcccg gctggacctc cacgttcgcg gacgcgttga tctcctacgc cgccgaacgc
      301 cgcgacatcg ttgcgctcac cgccgccatg gccggcccga ccgggctgac cgcattcggg
      361 aagaggtttc ccgacaggtt gttcgacgtc ggcatcgccg agcagcacgc gatgacctcg
      421 gccgccggtc tggcgatggg cgggctgcat ccggtggtgg cgatctactc gacattcctg
      481 aaccgggcgt tcgaccagat catgatggac gtcgcactgc acaagctgcc ggtcacgttg
      541 gtgctcgacc gttccggtgt caccgggccc gacggcgcca gccacaacgg catgtgggac
      601 ctgtcgatgc tgaacatcgt ccccggcatg cgggtggcgg caccgcgcga cgcggcgcgc
      661 ctctacgagg agctcggcga ggcactgcag gtcagcgacg gcccgaccac cctgcggttc
      721 cccaaaggtg atgtcggcga agacattccg gcgctcaggc ggcgagacgg catcgacatc
      781 ctcgccgtcc cggccgacgg cctttccgac gatgtgctgc tggtggcggt cggagcgttc
      841 gcgtcgatgg cgaagtcggt cgccgagcgg ctgcgcaacc agggcatcgg tgtgacggtg
      901 atcgatccgc gctgggtgct gccggtgccc gccgccatag gtgggctcgc ggaggcgcac
      961 aagctggtgg tcacgttgga ggacaacggc gtcaacggcg gcgtcggctc ggccgtgtcg
     1021 gccgcgttgc ggcgcgccga gatcgacgtg ccgtgccgcg acgtcggggt gccgcaggag
     1081 ttccaggcgc acgcgtcacg cggtgaggtg ctcgccgacg tcggcttgac cgatcaggtg
     1141 atcgcacgcc aggtgaccgg gtgggtggcc gcactgggca gtgcggtctc ggagcacgac
     1201 gtcagccagc tcgactgatc gaaacccgcg ccgcctcctc caacgtcgtc gacttgcgcg
     1261 gctgctcgaa gaacacttcc gctgtgcggt cgttgacctc gacggtggcg atcgtgttcc
     1321 cgaacagcgg gccgcagaca tcggtccacg tcatcggggg cgagggcact ccgcgccgac
     1381 gcacccacca ccgggtgacg gcggcggccg gacgggtcca gctgagtgtg aacaccggct
     1441 tgatgaacag cggtacatag ttgtgcacgg gcgagcacac cagctgatgc acggtcgctc
     1501 ccgtcggtga caccccgtcg acctcggcgc gtgctgcgta gctgtggtgc acgtcgcccg
     1561 acagcacgct gatcgtcgac ggcccgtcgg ggtggttcgc gatccgcgcg atcatctccc
     1621 cgagccgcac gaaggatttg tagaacgccg gccagtgctc gaaatcgcct gcctcgcgca
     1681 gcttctcgcc tagcgtgccg cgccagccgg tgcggtcggc cgcccgctcg ttgagggtct
     1741 cgagatcgcc gatcgccggt ggcatcaacc atggcaccga tgacgcgagc agcagatggt
     1801 cgagggtctc cgggtgctct tcggcctgag cctccaacca ccggaactcg ttgtcgccga
     1861 tcatcattcg ctcgccggac tcgaggatcc ggccgttgcg agagtcgatc atgatcagcc
     1921 gggtccgccc gaggtcccac cgatagctga accggatgcc cttgttgcag tccacctcgc
     1981 ggtcggcgcg ctcggccagc tccgcgaggt ggcaccaggt gtcaccgtcg gtggacacga
     2041 gacgccggta gtcgtcgttg cctgcgagct cgtccgggct catgttgccc aggtgttgat
     2101 acacccagta ggacgccagc ccggcccgta tccggtcgcg ccaccagggc agtttcgcca
     2161 tctctgcgcg ccaggacgcc gaggtgttcc agtcgtcgcg gatgtcgtgg tcgtcgaaga
     2221 tcatcgccgt cgggacggtc gacatcagcc accggatctc gggatccccc caggagtgcc
     2281 ggtagagccg ttcgtactcg gcgaagctga ccacctcgtc ggggggacgg tcgccgtgcc
     2341 tgctgcgccg ccgacctcgg acgcgccgct gctcgtcggg cgg
//
LOCUS       DASYLX010000983         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_722916,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000983 DASYLX010000000
VERSION     DASYLX010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722916"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VGP27_22905"
     CDS             complement(<1..686)
                     /locus_tag="VGP27_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV7854181.1"
                     /translation="MPDEPALQRRLGIADAVTIGLGSMIGAGIFVALAPAAAAAGSGL
                     LIGLAVAAVVAYCNAMSSARLAALYPQSGGTYVYGRERLGEFWGYTAGWSFVVGKTAS
                     CAAMALTVGIYAWPSHAHAVAVAAVVALTAINYAGIQKSALLTRVIVAVVLAVLAAVV
                     VAVAFGAGDADISRIFGGDTSVRGVLQAAALLFFAFAGYARIATLGEEVRDPARTIPR
                     AIPIALGITLA"
     gene            786..2051
                     /locus_tag="VGP27_22910"
     CDS             786..2051
                     /locus_tag="VGP27_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854182.1"
                     /translation="MARRAREQRIGLGRPTVDTGVRHRPKDRKAQIARASAEAFSALG
                     YHAVSMEAIASRVGISATALYRHYPSKYDLFRDAVLALGEQLVGCTEFVDDARTDAET
                     LRRLIAAITDTALVNRESGGLYRWEARYLRGDDQTTLDGQMRTVNRRIQQPLTALRPG
                     LSSRQRWMLSSSILSAIGSIVDHHAKLPAVHIRAVLAEVATAVSTADMPDLPDESVVE
                     EPERRRVGVEPSKYEALLTESVRLFNRKGYRDTTMEEIASTVGMPTSGIYRYFSGKND
                     ILAAIYRRAADRLSSEASSILVSVTDPEQALTGLVDAYVKRSFDHPELAYVYYTERLN
                     MAPADQKILGNLQRATVESWVRLVTAIRSDWTMGQARFAVHAAMALVIDVGRLMHYDN
                     SVASRETVGRLLDLTLLGRYRLRTTLPAR"
     gene            complement(2018..2293)
                     /locus_tag="VGP27_22915"
     CDS             complement(2018..2293)
                     /locus_tag="VGP27_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854183.1"
                     /translation="MPERKSGGSRAAALVGLGLAGAGVAHFVKPEVFDAVTGQAFPRE
                     TRKHVYIDGGVETAIGLGLTSRKTRPLAIIGLIGYGAYLAGNVVRNR"
     gene            complement(2312..2980)
                     /locus_tag="VGP27_22920"
     CDS             complement(2312..2980)
                     /locus_tag="VGP27_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEV7854184.1"
                     /translation="MPPGDYPPPPPPPGGYPPPPYYPAPPYYPGPGAYPGPAPGTNWW
                     AVISLVFGLIGGVLISVVCGIVGLNRARKGQGGRGMAIAGLVLSGLWALGIVALVVFL
                     VLFDKGTVSATEVKAGDCLKELPASGLVVTVDTAPCGEPHAGEIFSVMTMPDGDFPGQ
                     FAIEEYQNKCAPELADYSPEAAEDADVGLFVLYPSEDSWGQGDRTVTCIATTDSPRTG
                     ALKK"
     gene            complement(2983..3600)
                     /locus_tag="VGP27_22925"
     CDS             complement(2983..3600)
                     /locus_tag="VGP27_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV7854185.1"
                     /translation="MTLDDIAGIAHEPDGKPRGAVVLTHGAGGSRESALLKKICDEWA
                     RRGWLAVRYNLPYRRRRPKGPPSGSAATDQAGIVEAIELVRSMAKGPVVAGGHSYGGR
                     MTSMVVADGAAGVDALTLFSYPLHPPGKPERARTEHLPRIEVPTVFTHGTSDPFGSID
                     ELRAAAALIPASADVVEITGARHDLGAKSLDVPVLAVDAALRLLS"
     gene            complement(3597..>4265)
                     /locus_tag="VGP27_22930"
     CDS             complement(3597..>4265)
                     /locus_tag="VGP27_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEV7854186.1"
                     /translation="GFHEIPVDVIAGQIEAVASDIGIQAAKTGMLASSDIIGTVADTW
                     RSQGLAGSVPLIVDPVCASMHGDPLLHPSALDSLRAELFPLATLVTPNLDEVRLIVDI
                     DVVDDASQRDAARALHALGPQWTLVKGGHLRSSSESPDLLYDGTNFHEFGASRIDTGD
                     DHGAGDTLGSAVACALAHGYTVPDAVAFAKSWVTECLRAAYPLGHGHGPVSPLFRLSS
                     PQRS"
BASE COUNT          713 a         1463 c         1454 g          635 t
ORIGIN      
        1 gccagggtga tgcccagtgc gatcggaatc gcccgtggga tcgtgcgcgc ggggtcgcgc
       61 acctcctcgc cgagggtggc gatgcgtgcg taaccggcga acgcgaagaa caacagggcc
      121 gccgcctgca gcacgccacg cacgctggtg tcgccgccga agatccgcga gatgtcggcg
      181 tcccccgcac cgaacgcgac ggccacgacc accgcggcga gcacagcgag gacgaccgcg
      241 acgatgacgc gggtcagcag cgccgacttc tggatgcccg catagttgat cgccgtcaac
      301 gcgacgaccg cggcgaccgc gaccgcgtgc gcgtgcgacg gccacgcgta gatcccgacc
      361 gtcagggcca tggccgcgca cgaggcggtc ttgccgacca cgaagctcca ccccgcggtg
      421 taaccccaga actcgcccag tcgctcgcgg ccgtagacat aggtgccgcc ggactgcgga
      481 taaagcgccg ccagccgtgc cgatgacatc gcattgcagt acgcgacgac agcggcgacc
      541 gccagcccga tcagtagtcc cgaacccgcc gccgcagcag cgggtgccag cgcgacgaag
      601 atcccggcac cgatcatcga gccgaggccg atcgtcacgg cgtctgcgat gccgagccgc
      661 cgttgcagcg ccggctcatc aggcatgctc accggagccg atactaggtc aggcggcggg
      721 atcttcctcg aaccggctcg tggcccccgt tggaccggcc tacgcaggcg attagggtga
      781 tcgccatggc gcgccgcgca cgcgagcagc ggatcggtct cggccggccc actgtcgata
      841 cgggcgttcg gcatcggccg aaggaccgca aggcgcagat cgccagggcg tcggcagaag
      901 cgttcagcgc gctgggctat cacgcagtga gcatggaggc gatcgcatcg cgcgtcggca
      961 tctcggccac cgcactgtat cggcactacc cgagcaagta cgacctcttc cgcgatgcgg
     1021 tgctggccct cggcgagcag ttggtgggtt gcacggagtt cgtcgacgac gcgcggaccg
     1081 acgcggagac actgcggcga ctcatcgccg cgatcaccga caccgccctt gtcaatcgcg
     1141 aatcgggcgg gctctaccgt tgggaggcac gttatctgcg cggcgacgac cagaccacgc
     1201 tcgacggaca gatgcggacg gtcaaccgcc ggattcaaca gccgctcaca gcacttcggc
     1261 cggggctctc ctcgcgtcag cgctggatgc tgtcctcgtc gatcctgagc gccatcggca
     1321 gcatcgtcga ccatcacgcg aagcttccgg cggtccacat tcgtgccgtt ctggccgagg
     1381 tcgcgacggc cgtctcgacc gccgacatgc ccgatctgcc cgacgagtcc gtcgtggagg
     1441 agcccgagcg caggcgggtc ggcgtcgaac cgtcgaaata cgaagcgcta ctgacggaat
     1501 cggtgcggct gttcaaccgg aagggctacc gcgacaccac gatggaggag atcgcgtcga
     1561 cggtcggcat gcccacatcg ggcatctacc ggtacttctc cggcaagaac gacatcctgg
     1621 ccgcgatcta tcggcgggcg gccgaccggt tgtcgtcgga ggcctcgtcg atcctcgttt
     1681 cggtcaccga tccggagcag gcactgaccg ggttggtcga cgcgtacgtg aagaggtcgt
     1741 tcgaccatcc ggaactggca tacgtctatt acaccgagcg gctgaacatg gcgcctgccg
     1801 accagaagat cctgggcaac ctgcaacggg ccacggtcga atcgtgggtg cggttggtga
     1861 ccgcgatccg ctcggactgg acgatggggc aggcccgctt cgccgtacac gcggcgatgg
     1921 ctttggtgat cgacgtgggc aggctgatgc actacgacaa ctccgtggca tcgcgtgaga
     1981 cggtcggtcg actgctcgac ctgacgctgc tcggccgcta ccgattgcgg acgacgttgc
     2041 ccgccagata ggcgccgtag ccgatcaacc cgatgatggc caacggccgg gtctttcgcg
     2101 atgtgagacc gagaccgatc gcggtttcga cgccgccgtc gatgtagacg tgcttgcggg
     2161 tttcacgcgg gaacgcctgc ccggtgacgg cgtcgaacac ctcgggcttg acgaagtgcg
     2221 caaccccggc gcctgcgagt cccagaccta cgagcgccgc tgcgcgcgac cctccggact
     2281 tccgctctgg cacgtgaaat tcctttcgcc gttacttctt cagggccccg gtgcgagggc
     2341 tgtcggtggt cgcgatgcag gtcacggtgc ggtcgccctg gccccacgag tcctcgctcg
     2401 gatagaggac gaacaacccg acgtcggcat cctcggcggc ctcaggggaa tagtcggcca
     2461 actccggcgc gcacttgttc tggtactcct cgatcgcgaa ctggccggga aaatcaccgt
     2521 cgggcatggt catcaccgag aagatctcgc ctgcatgcgg ttcgccgcac ggcgcggtgt
     2581 cgacggtgac caccaagccg gaggcgggca gctccttgag gcagtcacct gccttcacct
     2641 cggtggcgct gacagtgccc ttgtcgaaga gcaccaggaa gaccacgagc gcgacgatcc
     2701 cgagcgccca caggcccgag agcaccagac ccgcgatggc catcccgcgc ccgccctgtc
     2761 ccttcctggc ccggttcaac ccgacgatgc cgcacacgac gctgatcagg acaccgccga
     2821 tgagaccgaa gaccagggag atgaccgccc accagttggt gccgggagcc ggccccgggt
     2881 acgcccccgg ccccgggtag tacggcggcg ccgggtagta cggcggcggc ggatatcccc
     2941 ccgggggtgg cggtggcggg ggatagtcgc ccggcggcat ggtcacgaga gcaaccgtag
     3001 cgccgcgtcg accgcgagaa ccggcacatc gagtgacttg gcgccgaggt catggcgggc
     3061 gccggtgatc tcgacgacgt ccgcggaggc cggaatcagc gccgcggcgg cgcgcagttc
     3121 gtcgatggaa ccgaacgggt ccgacgtgcc gtgggtgaac accgtcggca cctcgatccg
     3181 cggcaggtgc tcggtgcggg ccctctcggg cttgcccggc ggatgcagag ggtaggagaa
     3241 cagcgtcagc gcgtcgacgc ccgccgctcc gtcggcgacc accatcgacg tcatccggcc
     3301 gccgtatgag tggccgcccg cgacgacggg ccccttggcc atgctgcgga cgagttcgat
     3361 cgcctcgacg atgcccgcct ggtcggtggc cgccgacccc gacggcgggc ccttcggcct
     3421 gcgcctgcgg taaggcaggt tgtagcgcac tgcgagccaa ccgcggcgcg cccattcgtc
     3481 gcagatcttc ttcagcagcg cggactcccg gcttccgcca gcaccgtggg tgagcacaac
     3541 ggcgcccctc ggcttgccgt cgggctcgtg tgcgatcccc gcgatgtcgt cgagggtcac
     3601 gatcgctggg ggctcgacag ccggaaaagc ggtgacaccg ggccatgccc gtggcccaac
     3661 gggtaagcgg cgcgcagaca ttcggtcacc cacgacttcg cgaacgccac tgcgtccggc
     3721 acggtgtagc cgtgtgcgag cgcgcaggcc acggcggacc cgagcgtgtc gcctgcgccg
     3781 tgatcgtcgc cggtgtcgat tcgtgaggca ccgaactcgt ggaagttcgt gccgtcgtac
     3841 aacagatcgg ggctttccga cgacgaccgc agatggccgc ccttgaccag tgtccactgc
     3901 ggccccaacg catgcagcgc gcgggcggca tcgcgttggg aggcgtcgtc gacgacatcg
     3961 atgtcgacga tcagccgaac ctcgtccagg ttcggcgtca ccagcgtcgc gagcggaaac
     4021 agctcggcgc gaagggaatc cagcgcgctc gggtgcagta gagggtcgcc gtgcatcgat
     4081 gcgcacaccg ggtcgacgat gagcggcacg ctgcccgcaa ggccctgtga gcgccaggtg
     4141 tcggcgaccg ttccgatgat gtcggacgac gccagcatgc cggtcttggc cgcctgtatt
     4201 ccgatatcgc tcgccacggc ttcgatctgg cccgcgatga cgtcgacggg gatctcgtga
     4261 aagcc
//
LOCUS       DASYLX010000984         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3573580,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000984 DASYLX010000000
VERSION     DASYLX010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3573580"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGP27_22935"
     CDS             <1..300
                     /locus_tag="VGP27_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854187.1"
                     /translation="PGVYCTVELSIPRKTTSMIIPADAVVFDQNGLHVAVVESGIAHL
                     QKITIARDFGKEVEVHDGVRSGDQVILNPMIGLADGSKVAVRKSPTASQISSAEE"
     gene            complement(434..955)
                     /locus_tag="VGP27_22940"
     CDS             complement(434..955)
                     /locus_tag="VGP27_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854188.1"
                     /translation="MLPTIDVGMVPSGVDGIVVVDGVIVAVVPGMYVATVPGAVDGTS
                     TGTAVIEGNGGGGTADGADTGMVEPGKTVRADVSGCWENVSGGIAIGGSAAVAGAAET
                     DGVVPMVVLVSDVEGVAGITVAVGVPGVICPVGVEQLTTVPGVVGSEASGTGASVVPG
                     VPGCTVAENGLGP"
     gene            complement(1067..1240)
                     /locus_tag="VGP27_22945"
     CDS             complement(1067..1240)
                     /locus_tag="VGP27_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854189.1"
                     /translation="MALAGAIIPGVARFIVMLSAHADVFGVGITDVVESRLVATSCGM
                     SGWIVPGDGLDVI"
     gene            1777..>2125
                     /locus_tag="VGP27_22950"
     CDS             1777..>2125
                     /locus_tag="VGP27_22950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7854190.1"
                     /translation="MTKVLLIEDDSETAEEITAELADRGFEVEWSANGIEGLDKARSS
                     RPDAMIVDRLLPGMDGLTVIEALRKDQVRTPVLVLSALGAVDDRVRGLRMGGDDYLTK
                     PFAIVELVARVEAL"
BASE COUNT          419 a          679 c          632 g          395 t
ORIGIN      
        1 cccggggtct attgcaccgt cgaactgtcc attccgcgca agacaacatc gatgatcata
       61 ccagccgacg ccgtcgtatt cgatcagaat gggttgcatg tcgcggtcgt cgaaagcggc
      121 attgcccatc tgcagaagat cacgatcgcg cgtgacttcg gcaaggaagt cgaagtgcat
      181 gacggagtca ggtcgggcga tcaggttatc ctcaatccca tgatcggcct ggctgacggc
      241 agcaaggtcg cggttcgcaa atcgccgacc gcaagtcaga tcagttcggc ggaggaatga
      301 cgatgcagcg cttgctgttg gcaaccgcga cactgctgtt gggcgctcat gcctgcctgg
      361 cgcaagtttc caccatgggc actaccgcca tgggccttcc gtcgacgccg gcagcgatcg
      421 tgacctcacc cctttatggc ccgagtccgt tctccgcgac cgtgcagccg ggcacgcctg
      481 gaacgacgct ggcgccggtg ccgctggctt ccgacccgac aacgccgggg acggtcgtta
      541 gctgttccac accaacagga caaatcacgc cggggactcc gacagcgacc gttattcccg
      601 cgactccttc tacgtcggaa acgagcacca ccatgggaac gacgccatcg gtttccgcgg
      661 caccggcaac agcagcacta ccgccaattg cgattccgcc actgacattc tcccaacagc
      721 cagaaacatc cgccctgacc gtttttcccg gctcgaccat cccggtatcg gccccgtcgg
      781 cagtaccacc tccgccgttg ccttcgatta cggcggtgcc cgtactagtc ccatcgacgg
      841 caccgggtac ggttgccacg tacatgccgg gcaccacagc cacgatgacg ccatcgacca
      901 caacgatgcc gtcaaccccg ctggggacca ttcccacgtc aatcgtcggc agcacgaccg
      961 gaactatttc gccaattgct ccgctcggta gttcgtctac tactgtatgc agttccacgc
     1021 cgggaggccc accgacaaac ggcgcagctt tgccgctctc gacacctcaa attacgtcaa
     1081 gcccgtcccc cggcacgatc cagccggaca tgccgcagct tgtcgccacc agtctcgatt
     1141 cgacgacgtc cgtgatgcct accccgaata cgtcggcgtg cgccgagagc atgacgatga
     1201 atctcgctac gcccgggatg atggcgccgg ccaacgccac aggcgccgcg gcaacgccag
     1261 gggtatcacc gccgggctgc taaccagagc gggatgagat caggttgggc cgtgacgaca
     1321 ggccattctt cttttcacac cagggacaca acatatcgtg ggtgctgcga gccggggccc
     1381 attccatact gagcatgggg ttgttttcgc gatttctcgt ccggaccctg cggtgcgcca
     1441 gaagagcgtc ctcgccatgt tgcaagcagc gcctcaggtc acggcaattc taaagtgtaa
     1501 tttataaagt gctatttaat ggtgcagccc aacgatcagg cctatttcgt tgtagagcgg
     1561 ttctaccgcg cttgcccctc aggcggcggg tggccgctga gcggacggcg acgccacacc
     1621 ctttgccccc gaacggcggg cggcgctgtt cggctctcac agggaaaacc catggcagtt
     1681 caggtcgttg atatgacaat ggtctcgggc aagcgtgcag tcgttggggc aggaacctca
     1741 agtcctgcgg ccgtggccgc agccgggaat cgtttgatga caaaagtgct gctgatcgag
     1801 gacgacagcg agacagctga ggaaataaca gccgaactgg ccgatcgcgg ctttgaagtc
     1861 gaatggtcag ccaacggcat agagggcctc gacaaggcaa gatcgtcgcg gcccgacgcc
     1921 atgatcgttg atcgactgtt gcccggaatg gatggcctta cggttatcga ggcgctccgc
     1981 aaggaccagg tgcgcactcc ggtgctggtg ctgagtgcgc tcggcgcggt ggatgaccgg
     2041 gtgcgcggat tgcggatggg tggcgacgac tatctcacca aaccgttcgc gatcgtggag
     2101 cttgtcgcca gggtggaggc gttgc
//
LOCUS       DASYLX010000985         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_941933,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000985 DASYLX010000000
VERSION     DASYLX010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941933"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGP27_22955"
     CDS             <1..685
                     /locus_tag="VGP27_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV7854191.1"
                     /translation="EWRHRETRSITGATALVVGTGGIGREIAKLLRAAGMVVRGAGRR
                     AVADDPDFGEIVDSADLAAEAGWCDHLILAAPLTAATRGLVDARVLSAMKPDAHLVNI
                     ARGPMVDEVALLDALTQGSIGGATLDVFDTEPLPAGHPLWDAPNVTVTAHMSADAVGW
                     RESLAGQFATNVRNWLAGEPMINVVDKKLGYVSGAGGPATSRPGAGGPATSRPGAGGP
                     ATSRPGAAR"
     gene            682..2088
                     /locus_tag="VGP27_22960"
     CDS             682..2088
                     /locus_tag="VGP27_22960"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7854192.1"
                     /translation="MIASATELVEGYRKKLISPVEAAEAALDAIDAYDGRVNAFVLVD
                     RDGALAAAKDSEERWQSGEPLGPGDGVPTSIKDALWTCGWPTLRGSELIDAAGPWDED
                     APCVARLRETGAVILGKTTTPEYSWKGVTDSRRHGATGNPWDPAMTSGGSSGGSATAV
                     GLGMGPWSVGTDGGGSVRIPAGFTGTVALKPTYGLIPFYPPSPFGTLSHAGPMTRTVR
                     DAAALMDVITGFDARDWAAMPTPVTSFLSGLDDGVSGLRVGYSSDLGFVRNDPEVDVA
                     VRAAVDVLASAGAEVEEVDPGFSDPVDAFHVLWFAGEAKVLSSYGAIDDLADRIDPGL
                     HRTALIGLRYSASDYLDAMAVRMSLGGLMGRFHQRYDVLVTPTLPLPAFPVGMDVPGG
                     SVSPDWTSWTPYTYPFNLTQQPALSVPCGFTGAGLPIGLQIVGARHTDALVLRVGQAY
                     QSATGWHQRTPALLAEEV"
     gene            2090..2590
                     /locus_tag="VGP27_22965"
     CDS             2090..2590
                     /locus_tag="VGP27_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEV7854193.1"
                     /translation="MSRLITVSLDKRGVSCVARMLDDEAPRTCAAVWDALPLSAPVFH
                     GKYARNEIYTLLPAFAAADPGKENTTITPIPGDLCWFSFDSDVLGNPAYGYENTTGTG
                     TTGAIVDLALFYGRNNLLINGDQGWVPGNVFGAVVEGLDEMAEACQDLWMGGARGETL
                     SFQRRD"
BASE COUNT          410 a          837 c          935 g          411 t
ORIGIN      
        1 tgagtggcgg caccgcgaaa ctcgcagcat caccggcgcc actgctctgg tggtgggcac
       61 tggcggcatc gggcgcgaga tcgcgaagct tctgcgcgcg gcaggcatgg tcgttcgggg
      121 cgcgggccgc cgtgccgtcg ccgacgaccc cgacttcggc gagatcgtgg acagcgccga
      181 cctggcggcg gaagcgggct ggtgtgatca cctgatcctg gccgcgccgc tgaccgcagc
      241 gacgcgtggc ctcgtcgacg cgcgcgttct atctgccatg aagcccgacg cgcatctggt
      301 caacatcgcc aggggcccca tggtcgacga ggtggctttg ctggacgcgc tgacacaggg
      361 aagcatcggc ggggcgaccc tcgacgtgtt cgacaccgaa ccgctgcctg ccggtcatcc
      421 gttgtgggac gcgccgaatg tgaccgtcac cgcccacatg tcggccgacg ccgtcggctg
      481 gcgcgagagc ctcgccggcc agttcgcgac caacgtccgg aactggctgg ccggcgaacc
      541 gatgatcaat gtggtggaca agaagctcgg gtacgtgtcc ggagccggag ggccggcgac
      601 atcaagaccc ggagccggag ggccggcgac atcaagaccc ggagccggag ggccggcgac
      661 atcaagaccc ggagcggcgc gatgatcgcg tcggcgacgg aactggtcga gggctaccgg
      721 aaaaagttga tctcgcccgt cgaggcggcg gaggcggcac tcgatgccat cgacgcctac
      781 gacggccggg tgaatgcctt cgtccttgtc gatcgcgacg gtgcactggc cgccgcgaag
      841 gattcggagg agcgttggca gtccggagag ccgctcggac ccggcgacgg tgtgccgacc
      901 tcgatcaagg acgcgttgtg gacgtgcgga tggccgacgc tgcgcggcag cgagctgatc
      961 gatgcggcgg gaccgtggga cgaggatgcg ccgtgtgtgg cgcgtctgcg cgagacgggc
     1021 gccgtcatcc tcggcaagac cacgacgccg gagtactcgt ggaagggcgt caccgactcc
     1081 cgacgacacg gcgcaacggg taacccgtgg gatccggcga tgacgtcagg tggatccagc
     1141 ggaggcagcg ctacggcggt cgggcttggc atgggcccgt ggtcagtcgg cacggacggc
     1201 ggcggctcgg tccgcatccc tgcggggttc accggaacgg tcgcgctgaa gccgacatac
     1261 ggcctgattc cgttttatcc gccgagcccg ttcggcacgc tgtcgcacgc cggcccgatg
     1321 acccggaccg tgcgggacgc cgccgctctg atggacgtga tcaccggatt cgacgcccgc
     1381 gactgggcgg ccatgccgac gccggtgaca tcctttctgt ctggactcga cgacggggtg
     1441 tccggactgc gcgtcggata ctcatccgat ctcggcttcg tccgaaacga tcccgaggtc
     1501 gatgtcgccg tccgcgcagc cgtcgacgtc ctggcgagcg ccggggctga ggtggaggag
     1561 gtcgacccgg ggttcagcga cccggtggat gccttccacg tcctgtggtt cgccggcgag
     1621 gccaaggtgt tgtcgtccta cggggccatc gacgacctcg ccgaccggat cgatcccgga
     1681 ctgcaccgca ccgcgctgat cggattgcgc tactcggcgt cggactattt ggacgcgatg
     1741 gcggtccgga tgtcgctggg cgggctgatg gggcggttcc accagcgcta cgacgtgctc
     1801 gtgacgccaa cgctgccact gcccgcgttc cccgtcggca tggatgtgcc gggtgggtcg
     1861 gtgtcgccgg actggacgag ttggacgccg tacacctacc ccttcaatct gacacagcag
     1921 cccgccctca gcgtgccgtg cgggttcacc ggcgccggat tgccgatcgg attgcagatc
     1981 gtcggcgccc ggcacaccga cgcactggtg ttgcgtgttg gccaggccta ccagtcagcg
     2041 accggctggc accagcgcac accggcactt ctggccgagg aggtttgaga tgagcaggct
     2101 gatcacggtg tcgctggaca agcgcggagt gagttgcgtt gcccggatgc tcgacgatga
     2161 ggcaccccgg acctgcgccg cggtgtggga cgcgcttccg ctgtcggcgc cggtgtttca
     2221 cggcaagtac gcccgaaacg agatctacac gctgctgccc gcgttcgcgg ccgccgaccc
     2281 cgggaaggag aacaccacga tcacgccgat tccgggcgac ctgtgctggt tctcgttcga
     2341 ctccgatgtc ctcggcaatc cggcgtacgg ctacgaaaac accaccggca cagggacgac
     2401 cggtgccatc gtcgacctcg cgttgtttta cgggcgaaac aacctattga taaacgggga
     2461 ccagggctgg gtgcctggca atgtcttcgg cgcggtggtc gagggactcg acgagatggc
     2521 tgaggcgtgc caggacctgt ggatgggcgg cgcacgcggc gagacactga gctttcagcg
     2581 ccgcgactga cgg
//
LOCUS       DASYLX010000986         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2586340,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000986 DASYLX010000000
VERSION     DASYLX010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586340"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGP27_22970"
     CDS             <1..373
                     /locus_tag="VGP27_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854194.1"
                     /translation="TMSLREREFVVAARYMGVPNWRIIVRHILPNVASLLIIDTTLNV
                     GTAIIGETGLSFLGFGIQPPDVSLGTLIADGTNSATTFPWVFLFPAGVLVLIVLCTNL
                     IGDGIRDALDPSARPLRWRGK"
     gene            370..2208
                     /locus_tag="VGP27_22975"
     CDS             370..2208
                     /locus_tag="VGP27_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7854195.1"
                     /translation="MSLLEVRDLTVTFPTETERVAAVRGLNYHVDKGEVVALVGESGA
                     GKSAGAMAVVGLLPEYAEVSGSVRLHGDELLGLSDQRMSRVRGKAIGTVFQDPMSALT
                     PVYTVGDQIAEAIAVHNDETTRRAARTRAVELLELVGIGQPEQRARAFPHELSGGERQ
                     RVVIAIAIANDPDLLICDEPTTALDVTVQAQILEVLKTARDVTGAGVLIITHDLGVVA
                     EFADRALVMYAGRAVEVASVGGLYRDRRMPYTAGLLGSVPRLDAPQGARLVPIPGAPP
                     SLAALPPGCPFAPRCPLAIDDCRAAEPELITVAPNHTAACIRTEHVSGRSAADIYGVS
                     TAPRANDRAPQDPPVVLRVRNLVKTYKLTKGVVLRRTIGEVRAVDGVSFELQQGRTLG
                     IVGESGSGKSTTLHQILELSPPQEGSIEVLGLDVATLTTKTRRALRGDVQVVFQDPVA
                     SLDPRLPVFEVLAEPLAANGFDKKRIDDRVSELLGIVGLRREDASRYPAEFSGGQKQR
                     IGIARALALQPKILALDEPVSALDVSIQAGIINLLLDLQDRFGLAYLFVSHDLSVVKH
                     LAHRVAVMFKGKVVEQGDGDQVFTDPRNDYTRRLLAAVPRPEPDRG"
     gene            2219..>3307
                     /locus_tag="VGP27_22980"
     CDS             2219..>3307
                     /locus_tag="VGP27_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEV7854196.1"
                     /translation="MMLRRLAATLLVVGLTITACSSGDRGAPPAGGNAEVGKTNDINP
                     QDPATLRQGGTLRLAMGGYPSNFNSLHVDGNEADTGAMLRTTMPRAFRVGSDGSTTVN
                     TDYFTNIELTSESPQVVTYTVNPKAVWSDGAPITWEDIASQINATSGKNKDFRIASTN
                     GADRVGKVTRGVDDRQAVVTFAKPFSDWRGMFAGNTMLLPKSTTSDPEVFNKGQLDRP
                     GPSAGPFIVTDLDRTAQRITLKRNPKWWGTPPLLDGITYLVLDDKARIPALQNNTIDA
                     TGLVSLPELTTARNSSGISIRRAPGASWYHFTFNGAPGSILADKALRLAVAKGIDRQA
                     IANVTQRGLVDNPVPLNNHIFVAGQEGYQ"
BASE COUNT          601 a         1105 c         1063 g          538 t
ORIGIN      
        1 gacgatgagc ctgcgggaac gcgaattcgt cgttgcggca cggtacatgg gcgtgcccaa
       61 ctggcgcatc atcgttcggc acatccttcc gaatgtcgcg tcgctgctca tcatcgacac
      121 cacgctgaat gtcggcaccg ccatcatcgg cgaaaccggg ttgagctttt tgggattcgg
      181 catacagccg ccggatgtgt cactgggcac gctgatcgcc gacggcacca actcggccac
      241 gacgttcccg tgggtgttcc tgttccccgc gggtgttctg gtattgatcg tcctgtgtac
      301 caacctcatc ggtgacggaa tccgcgacgc gctcgacccg agcgcgcggc cactgcgatg
      361 gagaggcaaa tgagcctcct cgaggtccgc gacctcaccg tcaccttccc gaccgaaacc
      421 gagcgggtgg cggcggtccg cggactgaat taccacgtcg acaaggggga agtggtcgcc
      481 ctcgtcggcg aatccggtgc gggcaagtcg gcgggagcga tggccgtcgt cggactgctg
      541 ccggagtacg ccgaggtgag cggatcggtg cggctgcacg gcgacgagtt actcgggttg
      601 tccgaccagc ggatgtcccg ggttcggggc aaggccatcg gcacggtgtt ccaggacccg
      661 atgtcggccc tgacgccggt ctataccgtc ggcgaccaga tcgcggaggc catcgcggtg
      721 cacaacgacg agaccacccg cagggcggcg cgcaccagag ccgtcgagct gctcgagctc
      781 gtcggcatcg gccagcccga acaacgcgcc agggccttcc cgcacgagct gtccggcggc
      841 gagcgtcaac gtgtggtcat cgccatcgcc atcgccaacg atcccgacct actcatctgc
      901 gacgaaccga ccaccgcact ggacgtcacc gtgcaggcgc agatcctcga ggtgttgaaa
      961 accgcgcggg acgtcaccgg tgcaggcgtg ctgatcatca cccacgatct cggggtggtt
     1021 gccgagttcg ccgaccgcgc gctggtgatg tatgccggcc gcgccgtcga ggtggcctcg
     1081 gtcggcggac tgtaccgcga tcgccggatg ccctacaccg cagggctcct cggctcggta
     1141 ccgcgactcg atgctccaca gggtgcgcgg ctggtgccga ttccgggtgc cccgccgtcg
     1201 ctggcggcgc tgccgccagg atgcccgttc gcaccgcgct gcccgctggc gatcgacgac
     1261 tgccgtgcag ccgagcccga actgatcacg gtcgccccga atcacaccgc ggcctgcatc
     1321 cggaccgaac atgtcagcgg ccgcagcgcc gccgacatct atggcgtctc caccgcgccg
     1381 agagcgaacg accgggcgcc acaggaccct cccgtcgtgt tgcgcgtccg aaacctggtc
     1441 aagacataca aactgaccaa aggtgtggtg ctgcgccgca ctatcggcga agtgcgggcg
     1501 gtcgacggcg tgagcttcga gcttcaacag ggccgcacat tgggcatcgt cggcgagtcc
     1561 gggtcgggga agtcgacgac gctgcaccag atactggaac tgtccccgcc gcaggagggt
     1621 tccatcgaag tgctcggctt ggacgtggcc accctgacca cgaaaacccg acgggctttg
     1681 cgcggcgacg tgcaggtggt gttccaggat ccggtggcat cgctggaccc acggctaccg
     1741 gtcttcgagg tactggcaga gccattggcc gccaacggct tcgataagaa acgtatcgat
     1801 gaccgggtat ccgaactgtt ggggatcgtc gggttgcgtc gcgaggatgc cagccgttat
     1861 ccggccgagt tctccggcgg ccagaaacaa cgcatcggca tcgcccgtgc gttggcgctg
     1921 cagcccaaga tcctggcgct cgacgaaccg gtgtcggcgc ttgatgtgtc gatccaggcg
     1981 ggcatcatca atctgctgct ggacctgcag gaccggttcg gacttgcgta tctgttcgtc
     2041 tcccacgatc tgtcggtggt caaacatctc gctcaccgag ttgcggtgat gttcaagggc
     2101 aaggtggtcg aacagggtga cggtgaccag gtgttcaccg acccgcgcaa tgactacacc
     2161 cgcaggttgc tggccgcagt acctcgaccg gagcccgacc gaggctagag tctgtcggat
     2221 gatgctccga cgcttagctg caaccctcct ggtcgtcggt ctcaccatca ccgcctgctc
     2281 gagcggtgac cggggtgcac cgccggccgg cggtaacgcc gaagtcggaa agaccaacga
     2341 catcaacccg caggatcctg cgacgttgcg ccagggcggc actctgcgac tggcgatggg
     2401 cggttatccg tcgaacttca actcgttgca cgtcgacggc aacgaagccg ataccggcgc
     2461 gatgttgcgg acgacgatgc cgcgcgcatt ccgggtcggc tcggacggat ccacaacggt
     2521 caacaccgac tacttcacca acatcgagtt gaccagcgag agcccccagg tcgtcaccta
     2581 caccgtcaat cccaaggcgg tctggagcga tggcgcgccg atcacatggg aagacatcgc
     2641 ctcccagatc aacgcgacca gcgggaagaa caaggacttc cgcatcgcca gtaccaacgg
     2701 cgccgaccgg gtcgggaagg tgacgcgcgg cgtcgacgac cgccaggccg tcgtcacgtt
     2761 cgccaaaccc ttctcggact ggcgggggat gttcgccggg aacaccatgc tgctgcccaa
     2821 gagcacgacc tccgatccgg aagtgttcaa caagggacaa ctggaccgcc ccggaccctc
     2881 cgccggtccg ttcatcgtca ccgacctgga ccgcacggca cagcggatca cgttgaagcg
     2941 caacccgaaa tggtggggca cgccaccgct gctggacggc atcacctatc tggtactcga
     3001 cgacaaagcg cgcatccccg cactgcagaa caacacgatc gacgccaccg gattggtttc
     3061 gctgcccgag ttgaccaccg cgcggaacag ctcgggtatc tcgatccggc gtgcgccggg
     3121 ggcgagctgg tatcacttca cgttcaacgg tgcgccggga tcgatactcg cggacaaggc
     3181 gctgcggctg gcggtcgcca agggcatcga ccggcaggcc atcgccaacg tcacccaacg
     3241 cggcctcgtc gacaacccgg tgccgctgaa caaccacatc ttcgtcgccg ggcaggaggg
     3301 ttatcag
//
LOCUS       DASYLX010000987         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1601154,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000987 DASYLX010000000
VERSION     DASYLX010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601154"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGP27_22985"
     CDS             <1..248
                     /locus_tag="VGP27_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854197.1"
                     /translation="GGDSDWIHGTWKGDKFTERLTYDMTDPAIIGRAGFGVIDHVGRA
                     VCIDGNGTPAEGWGLFEHGALGRHDPSGFADWLTVAP"
     gene            complement(490..2088)
                     /locus_tag="VGP27_22990"
     CDS             complement(490..2088)
                     /locus_tag="VGP27_22990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854198.1"
                     /translation="MADERLREEQTDADERLREVGAPPARRAGGQTDADERLREEQTD
                     AEYSDVLIIGAGISGIGAAYRIHEKNPKLSYTVLERRERIGGTWDIHRYPGIRSDSDI
                     FTLSYPFEPWTRPEYVAEGADIREYLAQTARKHGIDDHIRFSTRVVSADWDSSTDTWT
                     VHTDVDGAPKTYRGRFLFFGTGYYNYDEPYRPDFPGLQDFAGEVVHPQQWPESLDYSG
                     KKLVVIGSGATAVSMTPSLTEKAGHVTMLQRSPSYVMSMPRINPVLEFIRRVLPLRAG
                     NAAARLYGKVVTVLIYAIARRAPKFSRRFIRNAAKRNLPENYPVDVHFNPRYDPWDQR
                     LCLILDNDFYQAIDNGRLDVVTDQIDHIDATGIVLRSGERIDADVIVTATGIQLQALG
                     GVALSIDGAEVKPQDRFVYKEHLLEDVPNAAWCVGYINASWTLRADLTARAVANLLAY
                     MGSHGYTHAYPHLGDVPMPEKPAWNINAGYVQRAAHALPKSGTHRPWNVRHNYVLDAI
                     DHRFDRIEESMVFGRVEANSAQSA"
     gene            complement(2129..>2392)
                     /locus_tag="VGP27_22995"
     CDS             complement(2129..>2392)
                     /locus_tag="VGP27_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854199.1"
                     /translation="YRPVLEIHGWGDLQPELNALSKQGRWAEMGTLINDEVLHTIAAC
                     GTAAEVAAHIRDRVDGISDQVCLYQPGPIAPEMLGEIVDALRG"
BASE COUNT          405 a          785 c          741 g          461 t
ORIGIN      
        1 acggcggcga ctcggactgg atccacggca cgtggaaggg cgacaagttc accgagcggc
       61 tcacctacga catgaccgat ccggcgatca tcgggcgcgc ggggttcggc gtgatcgacc
      121 acgtcggccg tgcggtgtgc atcgacggca acggcacacc cgccgagggt tggggtttgt
      181 tcgaacacgg tgcgctgggc cggcacgacc cgtcgggctt cgcggactgg ctgaccgtcg
      241 cgccgtagac cgctgtctaa cagcgatcgt caccgcgtca cagcggtaaa agcgatcaac
      301 tttttgcagg tgtcttctcg aacttccccc gcgaaacgtc gatcgatgtg acacctgtgg
      361 ctggggccaa gttttggaat tcgccttcgt cgcccttgac gcgccgagat cgacgttttg
      421 gagcgttctt ctcgcacttt ctcgcccaaa cgttggtttg ggcgaattag gtggtcgacg
      481 cgggctctcc taggccgact gcgcggagtt cgcctcgacg cgaccgaaca ccatcgactc
      541 ctcgatccgg tcgaagcggt gatcgatcgc gtccagcacg taattgtgcc gcacgttcca
      601 cggtcgatgc gttcccgact tcggcaacgc gtgcgcggcc ctctgcacgt agcccgcgtt
      661 gatgttccac gcaggcttct ccggcattgg cacgtcaccg agatgcgggt aggcatgcgt
      721 atagccgtgg gaacccatgt acgccaacaa gttcgccacc gctcgtgcgg tcagatccgc
      781 acgaagcgtc catgaggcgt tgatgtaccc gacgcaccat gccgcattcg gcacgtcctc
      841 gagcaggtgc tccttgtaga cgaatcggtc ctgcggcttg acctccgcac catcgatact
      901 caacgcgacg ccgccgagcg cctgcaactg gataccggtc gcggtgacga tgacatcggc
      961 atcgatccgc tcaccggagc gcagaacgat gccggtcgcg tcgatgtgat cgatctgatc
     1021 ggtcaccacg tccaatcggc cattgtcgat cgcctggtag aagtcgttgt cgaggatcag
     1081 gcacaatcgc tgatcccacg ggtcgtagcg cgggttgaag tgcacgtcga ccggatagtt
     1141 ctctggcaga ttgcgtttgg cggcattgcg gatgaaccgt ctgctgaact ttggcgccct
     1201 gcgggcgatc gcgtagatca acaccgtcac gacttttccg tacagccggg ccgcggcgtt
     1261 tccggcgcgc aacggaagca cccgccggat gaactcgagg accgggttga tccgcggcat
     1321 cgacatcaca taactcggcg aacgctgcag catcgtcaca tgaccggcct tctccgtcag
     1381 cgacggggtc atgctgaccg ccgttgcgcc gcttccgatc accaccagtt tcttgccgct
     1441 gtagtccaat gactccggcc actgctgggg atgcacgact tcgccggcga aatcctggag
     1501 cccgggaaag tcgggacggt agggctcgtc gtagttgtag tagccggtgc cgaagaacag
     1561 gaacctgccg cggtacgtct tcggcgcgcc gtcgacatcg gtgtgcaccg tccaggtatc
     1621 ggtggacgaa tcccagtcgg ccgacaccac ccgggtgctg aaccggatgt ggtcgtcgat
     1681 gccgtgcttg cgcgcggtct gcgccaggta ctcgcggatg tcagcgcctt cggcgacgta
     1741 ctccggtcgc gtccacggct cgaacggata gctcaacgtg aagatgtcac tgtcagaccg
     1801 gataccggga tagcgatgga tgtcccaggt gccgccgatc cgttcccgac gctccagcac
     1861 cgtgtaggac agcttcgggt tcttctcgtg gatgcgatac gccgcgccga tcccagagat
     1921 tcccgcgccg atgatcagaa cgtccgagta ctcggcgtca gtctgctctt cgcgcaagcg
     1981 ctcatcggcg tcagtctgtc cccctgccct tcgggcggga ggtgccccca cttcgcgcaa
     2041 gcgctcatcg gcgtcagtct gctcttcgcg caagcgctca tcggccatct agacctccct
     2101 tggtccgccc cggtaccacg atatgtggtc agccgcgcag cgcgtcaacg atctcgccca
     2161 acatctccgg cgcgatcgga cccggctggt acaggcagac ctggtcggat atgccgtcga
     2221 cccggtcccg gatatgggcc gccacctcgg ccgccgtgcc gcaggcagcg atggtgtgca
     2281 gcacctcgtc gttgatgagc gtgcccatct ccgcccaccg gccctgcttg gacagcgcat
     2341 tgagctcggg ctgcaggtca ccccacccgt ggatctcgag caccgggcgg ta
//
LOCUS       DASYLX010000988         5996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3574087,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000988 DASYLX010000000
VERSION     DASYLX010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574087"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGP27_23000"
     CDS             complement(<1..424)
                     /locus_tag="VGP27_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854200.1"
                     /translation="MTSDGVAPMILLSEPGQPAAEELSAIDRIRNAAMKILATHGASG
                     TSLRAVAAAAGVSLGSVQHHFATKAGLVKAVDDWALAVVVTAITQPIPDTTPDSVAEI
                     GDRVTRMIAEQPEIADYIGRALIDGRPVGDMIFDSLLNS"
     gene            521..790
                     /locus_tag="VGP27_23005"
     CDS             521..790
                     /locus_tag="VGP27_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7854201.1"
                     /translation="MVLDESATFACTRDPDRWTTRADDGAKALCRSCPRRWACAQEAC
                     RTQGAEGVWAGILIPEAGRGRRHALKQLRELAEQNGFPVVQVTQI"
     gene            876..1112
                     /locus_tag="VGP27_23010"
     CDS             876..1112
                     /locus_tag="VGP27_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854202.1"
                     /translation="MGERPAPTDTTTSEALATTALFLNVASVIALAICLASWGASNAA
                     LATAAGVGALLTFAASLVCFAKQASEAEPQPVTA"
     gene            complement(1131..2603)
                     /locus_tag="VGP27_23015"
     CDS             complement(1131..2603)
                     /locus_tag="VGP27_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7854203.1"
                     /translation="MPDGLPQRANLQHLQRGNVPDRLLASWQRSEDYGIPLDAVEPAF
                     TGTDDLSSLFFLCGNEVLVDLHRTLANEPVSLMLTDAEGVVLSRLSGDRTLLKSLDDV
                     HLAPGFAYSEREVGTNGLGLALADRTPTLVRADQHYARSLCTFTCAAVPVLDPVSGRL
                     EGSVNLTTWSRSSSDLLLALARSAASNTTALMLARSSGRLPRQMPRGQVFRVEMPRLE
                     PGSGTLHSLSDAWNDAVAHAERGARAGGVVAAVGEPGSGRATALAQAARHLFPRDRIL
                     SASAPAPTDVQTWLGLWAPELGKDHTAVIVRDVDMLPAWVAEQLRDLIARSADSGSVP
                     VALTAERFEDIPAALAGLVDTVVPVPSLRDRPDDVLPLANHIAMRIRGREIGFSTTAA
                     RAMRCYDWPGNVDELSRVVSAAAGRADVVDIGMLPPEVLAGNTRRLSRIQAFERGEIV
                     RVLNSDGLTMAEVARELGMSRATLYRKVAQYNIQVPRERA"
     gene            2759..3796
                     /locus_tag="VGP27_23020"
     CDS             2759..3796
                     /locus_tag="VGP27_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7854204.1"
                     /translation="MYQKDNDSYFIVDAHVALWDARPENQRNIHGKQFIDCFYDYHRN
                     LSPESQVWGYDEYLYQGGERLMKDLFADGYVDHAIFQPAALGEFYYRGFGQTDEASDL
                     AAKHPDKLTYNHHWDPRNGEVGMDQLRVDAERFGLKGVKLYTAEWHGDSRGWRLDDPW
                     AYRYFELCRELGIRNIHVHKGPTIRPLDRDAFDVADVDHVATDFTDLNFIVEHCGLPR
                     LEDFCWIATQEPNVHAGLAVAMPFIHTRPRYFAQIVGELLYWIGEDRIQFASDYAIWT
                     PRWLVEAFVDFQIPEDLGEYAPITTEQKKKILGLNAAKMYDIAVPAELQIPSADEPAV
                     GPRGADNLVGA"
     gene            3798..4535
                     /locus_tag="VGP27_23025"
     CDS             3798..4535
                     /locus_tag="VGP27_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEV7854205.1"
                     /translation="MGRYETAYSALDAVVDPELDEPITTLGFVRSLVVSPGGNVKVHL
                     RLPTSFCSPNFAYLMASDAKDALTKLDWTRRVVVELDDHHDSPIINAGLAADAGYRGT
                     FGPETEDSLDELRVTFRRKAHTAAMERCLTELLRADTQRSESSLGEVQLGDLPDGATT
                     EALLQRRRALGLPTQPDALVMVDHLGGGYARDDVPLALRRARSTRISIDGNAHFCRGL
                     LRTRYGETEANTFIPLNTLMTKEGALR"
     gene            4541..5566
                     /locus_tag="VGP27_23030"
     CDS             4541..5566
                     /locus_tag="VGP27_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7854206.1"
                     /translation="MRAVQVVGYHQNLEMKEAPVPTPTGPFDVVVKIGGAGVCRTDLH
                     ILEGQWAEKSQVELPYTIGHENAGWVDAVGSAVTNVREGDKVIVHPLITCGLCRACRS
                     GDDVHCEANAFPGIDTDGGYADYLKTSARSVVKIDDSLEPSDVAALADAGLTAYHAAA
                     KAARRLTPRDKCVVIGAGGLGHIGIQVLKALSPAELIVVDRNPEALKLAEAIGADHGV
                     AADGTQVDQVLSLTGGNGAEVVVDFVGEGGATSEGVAMLRRAGDYHVVGYGENINVPT
                     IDIVSTEINFIGNLVGSYNDLCDLMALAASGAVNLHTQKYALDDFQSAITDLDNGNVR
                     GRAILTP"
     gene            5586..5915
                     /locus_tag="VGP27_23035"
     CDS             5586..5915
                     /locus_tag="VGP27_23035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7854207.1"
                     /translation="MIFIVVKFKPKAEYVESFPDFVAEFTEATRNEPGNMFFDWSRSL
                     DDPSEYVLVEGFRDGDAGNKHVTSEHFKKFVAEAPKVLAATPLIISHTIDADGWSEMG
                     EMKVGQR"
BASE COUNT         1092 a         1986 c         1955 g          963 t
ORIGIN      
        1 cggagttcag gagggagtcg aagatcatgt ctccgaccgg cctgccgtcg atcaaggcgc
       61 ggccgatgta atccgcgatc tcgggttgct cggcgatcat ccgggtcacc cggtcgccga
      121 tctcggcgac cgagtccggc gtggtgtccg gaatcggttg cgtgatcgcg gtgaccacca
      181 ccgccagtgc ccagtcgtcc accgccttga cgagcccggc cttcgtggcg aaatggtgtt
      241 gcaccgatcc gagtgacacg ccggcggccg cggcgaccgc ccgcagcgac gtaccggagg
      301 cgccgtgtgt ggccaggatc ttcatcgccg cattgcgaat ccgatcgatg gccgaaagtt
      361 cctccgcggc cggctgcccg ggctccgaca gcaggatcat cggcgcaacg ccatcgctgg
      421 tcacatccgt aggttagaca atacagtcgt atcgtgtcta cgatacaagc gtattgtcga
      481 tcccaatgga caacggaagg tcagagactc cgtggaccta atggtgttag acgagtcggc
      541 tacgttcgcg tgcacgcgcg accccgatcg atggacaacg agggcagacg acggcgccaa
      601 agccctgtgc cgctcgtgcc cacgtcgctg ggcatgcgcg caggaggcct gccggaccca
      661 gggcgcagaa ggcgtatggg caggcattct gattcccgag gccgggcgtg gccggcgcca
      721 cgcgctcaag caactgcgcg aacttgctga gcagaacggt ttccctgtcg tgcaggtgac
      781 ccaaatctga gctgtcgacg ggcggtgagg tttgcttgcc gccggaacgg ggcaacatat
      841 gtccgtcaga ttcggttagc cacgaggaga ccgtcatggg cgagcgcccc gctcccacag
      901 acacgactac gtccgaggcc cttgcgacca ccgcgctttt cctcaacgtc gcgtcggtga
      961 tcgcgcttgc catctgcctc gccagctggg gcgcttcgaa cgccgcgctc gccacggccg
     1021 ccggcgtcgg tgcattgctg accttcgcag ccagcctggt ctgtttcgcc aagcaggcaa
     1081 gcgaagccga gccgcaaccc gtcacggcat aacgacgacg gcccacccga tcaggcccgc
     1141 tcgcgcggaa cctggatgtt gtactgagcg actttccggt agagggtagc gcggctcatg
     1201 cccagctcgc gggcgacttc cgccatcgtc aacccgtccg agttgagcac ccggacaatc
     1261 tctcctcgtt caaacgcttg gatgcgcgac agacgacggg tgttgccggc caacacttct
     1321 ggcggcagca tcccgatgtc caccacatct gcccggccgg ccgccgccga gaccacccgc
     1381 gacagctcgt cgacgttgcc cggccagtcg tagcacctca tcgcgcgtgc cgcggtcgta
     1441 ctgaagccga tctcccggcc gcggatacgc atcgcgatgt ggttggccaa cggcagcacg
     1501 tcgtcgggcc ggtcgcgaag agatggcacc gggacgaccg tgtcgaccag tccggccagc
     1561 gcggcaggga tgtcttcgaa ccgctcggcc gtcagcgcga ccgggacact gcccgagtcg
     1621 gcgctgcgcg caatcaggtc acggagctgc tcagccaccc aggccggcag catgtcgacg
     1681 tcgcgcacga tgactgccgt gtggtccttg cccagctcgg gcgcccatag ccccaaccaa
     1741 gtctgcacgt ccgtcggcgc cggtgcgctg gccgacagaa tgcggtctcg cgggaacagg
     1801 tggcgggccg cctgcgccaa cgccgtcgcc cggcccgagc ccggctcgcc gacggccgca
     1861 acgacgccgc cggcccgcgc gccgcgttcg gcgtgtgcca ccgcgtcgtt ccacgcgtcc
     1921 gacaacgaat gcagcgttcc cgagccgggt tccagacgcg gcatctccac ccggaatacc
     1981 tgtccacggg gcatctgtcg cggaaggcgg ccgctcgacc gggccagcat cagcgcggtg
     2041 gtgttgctcg ccgcagaacg cgcgagcgcg agcaggagat cgctcgacga gcgcgaccac
     2101 gtcgtcaggt tgacgctgcc ctccagccgg ccggacaccg ggtccagcac cgggacggcg
     2161 gcgcaggtga atgtgcacag gctacgggcg tagtgctggt cggcgcggac cagcgtcggc
     2221 gtccggtccg ccaacgccag cccgaggccg ttcgtgccga cctcgcgttc ggaataggcg
     2281 aaccccggtg cgaggtggac atcgtcgagc gatttgagca gcgtgcgatc gcccgacaac
     2341 cgcgacagca ccacgccttc ggcgtcggtg agcatcaggc tgaccggttc gttagccagc
     2401 gtgcggtgaa ggtcgaccag gacctcgttg ccgcagagaa aaaacagcga actcaggtcg
     2461 tcggtgccgg tgaacgccgg ctccaccgca tccagtggaa tgccgtaatc ctcgctgcgt
     2521 tgccacgacg cgagcaatcg atccggcacg ttgccgcgct ggagatgctg caggtttgct
     2581 cgttggggca gcccgtccgg catgtgatcc acgttacaaa cagtggcaag acccgcaggt
     2641 caaaacaccc ccaccggtgt ctcagattga gacatcacgc ccctcggctg ccgcgcccca
     2701 cgctcctagg ctcgccaata ggttgtgaca gtggtcacac gagagaagga cggcccgcat
     2761 gtatcagaag gacaacgaca gctatttcat cgtcgacgca cacgtagccc tgtgggacgc
     2821 caggcctgag aatcaacgca acattcacgg caagcaattc atcgactgct tctacgacta
     2881 ccaccgcaac ctctcgccgg aatcccaggt gtggggctac gacgagtacc tctatcaggg
     2941 cggcgaacga ctgatgaagg acctgttcgc cgacggctac gtcgatcacg ccatctttca
     3001 gcccgccgct ctcggcgagt tctactacag gggtttcggt cagaccgacg aagcatccga
     3061 cctggccgcc aagcaccccg acaagctgac ctacaaccac cattgggacc cccgcaacgg
     3121 cgaggtcggc atggaccagc tgcgggtgga cgccgaacgg ttcgggctga agggggtcaa
     3181 gctctacacc gccgaatggc acggcgactc ccgcggctgg cggctcgacg acccgtgggc
     3241 ataccggtat ttcgagctgt gccgcgagct ggggatccgc aacatccacg tgcacaaagg
     3301 gccgaccatc cggccgctgg accgagatgc gttcgacgtc gccgacgtcg accatgtcgc
     3361 caccgacttc accgacctga acttcatcgt ggagcactgc gggctgccgc ggctggagga
     3421 cttctgctgg atcgcaaccc aggagccgaa cgtgcacgcc ggccttgccg tggcgatgcc
     3481 gttcatccac acccggccgc gatacttcgc gcagatcgtc ggcgagctgc tgtactggat
     3541 cggcgaggac cggatccagt tcgcgtcgga ctacgcgatc tggacgccgc gctggctcgt
     3601 cgaggcgttc gtcgacttcc agatccccga ggatctcggc gagtacgcgc cgatcaccac
     3661 cgaacagaag aagaagatcc tcggcctcaa cgccgccaag atgtacgaca tcgcggtacc
     3721 cgccgagctg cagatcccct cggccgacga gcccgccgtg gggccgcgcg gcgccgacaa
     3781 cctcgtgggc gcgtgacatg ggccgctacg aaaccgctta cagcgcattg gatgcggtgg
     3841 tcgatcccga actcgacgag ccgatcacca cgctcgggtt cgtccgctcg ctggtggtgt
     3901 cgcccggggg caacgtcaag gtgcacttgc ggctgccgac gtcgttctgc tcgccgaact
     3961 tcgcgtatct gatggcgtcg gacgccaaag acgctctgac aaagcttgac tggacgagac
     4021 gggtggtcgt cgaactggat gatcaccatg actccccgat catcaacgcc ggtctggccg
     4081 cggacgccgg ttatcgaggc acgttcggcc ccgagaccga ggacagcctc gacgagttgc
     4141 gggtgacgtt ccggcgcaag gcccatacgg cggcgatgga gcgctgcctg accgagctgc
     4201 tccgagcaga cacgcaacgg tccgagtcgt cgttgggcga ggttcaattg ggggatctgc
     4261 ccgacggcgc gacgacggag gcactgctgc aacgccgccg cgcactcgga ttgccaacgc
     4321 aaccagatgc tttggtgatg gtcgatcacc tgggcggtgg ctatgcgcgt gacgatgtgc
     4381 cgttggcttt gcggcgggcc cgttcgaccc gcatctccat cgacggcaac gcccacttct
     4441 gccgcggtct gttgcggacg cgctacggcg aaaccgaggc gaacactttc attccgttga
     4501 acacgctcat gaccaaagaa ggagcactgc gatgagcacc atgcgagccg tccaggtcgt
     4561 cggctaccac cagaacctcg agatgaaaga ggcacccgtc ccgacgccga ccgggccctt
     4621 cgatgtcgtg gtcaagatcg gcggcgccgg ggtttgtcgc accgaccttc acatcctcga
     4681 gggccaatgg gcagagaagt cgcaggttga actgccgtac acgatcggcc acgagaacgc
     4741 cgggtgggtc gatgcggtcg ggtccgcggt gaccaatgtg cgcgagggcg acaaggtgat
     4801 cgtgcacccg ctcatcacct gtgggctgtg ccgcgcgtgc cgctcgggtg acgatgtgca
     4861 ctgcgaggcc aacgcgtttc cggggatcga caccgacggc ggctacgccg actacctgaa
     4921 gacctcggcg cgcagtgtcg tgaagatcga cgactcgctg gagccgtccg atgttgcggc
     4981 gctggccgat gctgggttga cggcgtacca cgcggcagcc aaggcggcgc ggcgcctgac
     5041 gccccgggac aaatgcgtgg taattggcgc gggcggcctt ggccacatcg ggattcaggt
     5101 gctcaaggcg ctctcccccg ccgagttgat cgtcgtcgac cgaaatccgg aggcgctcaa
     5161 actcgccgag gcgatcggcg ccgaccacgg cgtggcggcc gacgggacgc aggtggatca
     5221 ggtgctgtcg ctgacgggcg gtaacggcgc cgaggtcgtc gtcgacttcg tcggcgaagg
     5281 cggcgcgacg tcggaaggtg tggcgatgct gcggcgggca ggcgactatc acgtcgtcgg
     5341 ctacggcgag aacatcaacg tgccgacgat cgacatcgtc tccaccgaga tcaacttcat
     5401 cggtaacctg gtcgggtcct acaacgacct gtgcgacctg atggcgctcg cagcgagcgg
     5461 cgcggtcaac ctgcataccc agaagtacgc tctcgacgac ttccagtcgg cgataaccga
     5521 tctggacaac ggcaacgttc gtggccgcgc cattctcacg ccttagagag aaggaatgat
     5581 ctgcagtgat tttcatagtg gtcaagttca agccgaaagc agagtacgtc gagagcttcc
     5641 ccgacttcgt cgccgagttc accgaggcga cccgcaacga gccgggcaac atgttcttcg
     5701 actggtcgcg cagtctcgac gacccgtcgg aatatgttct ggtggaggga ttccgcgacg
     5761 gcgatgcagg gaacaagcac gtcaccagcg agcacttcaa gaagttcgtc gccgaggcac
     5821 cgaaggtcct ggccgccact ccgctgatca tcagccacac gatcgacgcg gacgggtggt
     5881 cggagatggg cgagatgaag gtcggccagc gttaggcagg cctcgcgagc tccatggccg
     5941 tgggcacggc ggtgctcgcg gacgaaaaac ctgcggcacg gcggatttcg ccgaac
//
LOCUS       DASYLX010000989         4550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2368698,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000989 DASYLX010000000
VERSION     DASYLX010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368698"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGP27_23040"
     CDS             complement(<1..258)
                     /locus_tag="VGP27_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854208.1"
                     /translation="MPDDERTGDDLAEDDGPVFSGYGVASAALGLLSVAAIVLALMIW
                     SGHRSDADERRYRTEVLKVAADWTTVLINMNSGDVDTSLQRL"
     gene            468..626
                     /locus_tag="VGP27_23045"
     CDS             468..626
                     /locus_tag="VGP27_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854209.1"
                     /translation="MTANTTTSTRRVAAAAVAQHDCADRGCLAAMLGIELGDATAGNA
                     EEGSSSSR"
     gene            673..2667
                     /locus_tag="VGP27_23050"
     CDS             673..2667
                     /locus_tag="VGP27_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEV7854210.1"
                     /translation="MTVEATRSGIDLSHVDQQARPQDDLFGHVNGRWLADYTIPADRA
                     TDGAFRSLYDRAEEQIHDLITGAAAAADAEKGTDQQRIGDLYASFMDEDTVQRIGVQP
                     LLDELATIDNASDADALAAVIGGLQHTGVGGGTGAYIDTDSKDSNRYLLHLSQSGIGL
                     PDESYYRDEQHADILAAYPRHIAAMFGLVYGEEPQPGEWQATAERIVALESKLAAAHW
                     DVVKRRDADLTYNLRKFADLPAEAPGFDWAGWITAMGTTPETAAELVVRQPDYLTAFA
                     ALWAGEDIEDWKRWARWRLIHARASLLTDDLVAEDFDFYGRTLSGTESIRERWKRGVS
                     VVESLMGDALGKLYVERHFPPGAKARMDELVANLREAHRVSINQLEWMTPETRQRALA
                     KLDKFTPKIGYPPKWRDYSTLVIERDDLYGNYRRGYALSSEREFAKLGGPVDRDEWFM
                     TPQTVNAYYNPGMNEIVFPAAILQPPFFDADADDAANYGGIGAVIGHEIGHGFDDQGA
                     KYDGDGNLVDWWTDDDRTEFGARTKALIEQYDQYVPRALSNGYHVNGAFTVGENIGDL
                     GGLSIALLAYQLSRNGEPAPVIDGLTGVQRVFYGWAQVWRTKSRDAESIRRLAIDPHS
                     PPEFRCNGVIRNMDAFYEAFEVDEDDALYLEPERRVRIWS"
     gene            complement(2651..3133)
                     /locus_tag="VGP27_23055"
     CDS             complement(2651..3133)
                     /locus_tag="VGP27_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854211.1"
                     /translation="MTDVLRKAHLLSTAFGLVMVAAAGFQTSGPALVLAMIAAGTVVA
                     GIVLRVAATPAILLAAAAIVVSDAPPMPAALAGLSAACYLVLRYTEGGSARVVGASAT
                     AMLAALGFTFIGVLAASFPFELPWLPLLAPLAVFGIYVLATRPFFQAGEGKLEISSRS
                     "
     gene            complement(3130..4440)
                     /locus_tag="VGP27_23060"
     CDS             complement(3130..4440)
                     /locus_tag="VGP27_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7854212.1"
                     /translation="MIETRRTEVDLRWHASPLTRAVATCAGVVLVAALIGSRWQLIAF
                     VAPLLGVLCSIGWQRGLPKVFVHAAPGLQRCFEGEQTQVSVWATAEPADLTVALGITG
                     VDGMRLDVIPEPSGRQTVTAAADRWGRYPIRATVDVVAPGGLLSGTATVDVADAFVFP
                     LAPPQSTAIPKTELLDRLGTHLTRHIGPGVEYADIRPYVPGDQLRTVNWPVSARRGSL
                     HITQRLTDRAADVVVLIDMYAQPPGPATEATERALRGAVQVVQSALRSGDRAGVVALG
                     GGRPRWLGADIGRRQFYRVLDTLLGAGDEFEPTTGSLAPRAALPPGAIVVAFSTMLDT
                     EFALSLIDLRKRGHTVVAVDVLEGAPFGEGTAHARSGKGTAHARSGEEIDPLFTRMWM
                     MQRSFMYRDMGTVGVDVVSWPSENTLDQAMRLVPEHRRPVRRRR"
     gene            complement(4437..>4550)
                     /locus_tag="VGP27_23065"
     CDS             complement(4437..>4550)
                     /locus_tag="VGP27_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV7854213.1"
                     /translation="TMAHRISLKPEMWVRRVHGADVVEELLRRLPVPRART"
BASE COUNT          810 a         1578 c         1527 g          635 t
ORIGIN      
        1 caacctctgc aggctggtgt cgacgtcgcc ggagttcatg ttgatcagga cggtggtcca
       61 gtcggcggcg accttgagca cctcggtccg gtagcggcgc tcgtcggcgt ccgaccggtg
      121 gcccgaccag atcatcagtg caaggacgat tgccgccacc gagagcaggc cgagcgcggc
      181 ggaggccacg ccgtacccgg agaagaccgg gccgtcgtcc tcggcgaggt cgtcgccggt
      241 gcgctcgtcg tcaggcatgg ccgtgagggt acctaaccgt gattccggca cgatcgccgt
      301 cgccgcgacc gccgtccact ccttgagcac aatgcgcaaa aggcggagat cgagtctttt
      361 gcggtttgcg cgcacaaggg agccacttcg accgcggaca catagcttgt ggtcgccgga
      421 gcgaaccggc gaacacccga atcgaaaacg caaacaggag aattgacatg acagccaaca
      481 ccaccacctc gacccgtcga gtggccgccg cagcggtcgc gcaacacgac tgcgcggacc
      541 gcggctgcct ggccgcgatg ctcggaatcg agctgggtga cgccactgca ggcaacgctg
      601 aggaaggttc ctcatcgagc cggtgatcac cttgcagagg aggcactctc gcacttctgg
      661 aagaatgtgg gggtgacggt agaagcaact cgctcgggca tcgacctgag ccacgtcgat
      721 cagcaagccc gcccgcaaga cgacctgttc ggccatgtga acggccgctg gctggccgac
      781 tacacgatcc ccgccgaccg ggccaccgac ggcgcgttcc gctcgctgta cgaccgcgcc
      841 gaggagcaga tccacgatct gatcaccgga gcggctgcgg cggcagacgc cgagaagggc
      901 accgatcagc agcgcatcgg cgatctgtac gcgagcttca tggacgagga cacggtgcag
      961 cgcatcggtg tgcagccgct gctcgacgag ctggcgacga tcgacaacgc ctccgacgcc
     1021 gatgcgctgg ccgcggtgat cggcggtctg cagcacaccg gtgtcggcgg cggcaccggc
     1081 gcctacatcg acaccgattc caaggactcc aaccgctacc tgctgcacct gagccagtcg
     1141 gggatcgggc tgcccgacga gtcgtactac cgcgacgagc agcacgccga catcctggcc
     1201 gcctatccgc gccacatcgc cgcgatgttc gggctcgtct acggcgagga gccgcagccg
     1261 ggagagtggc aggccaccgc ggagcgcatc gtggcgctgg agtcgaagct ggcggccgcg
     1321 cactgggacg tcgtcaagcg gcgcgacgcc gacctcacct acaacctgcg caagttcgcc
     1381 gacctgcccg ccgaggcgcc gggcttcgac tgggccggct ggatcacggc gatgggcacc
     1441 acaccggaga cggcagccga actcgtcgtg cgccaacccg actacctcac cgcgttcgcc
     1501 gcactgtggg caggcgagga catcgaggac tggaagcgct gggcccgctg gcggctgatc
     1561 cacgcccgcg cttcgctgct caccgacgat ctcgtcgccg aggacttcga cttctacggc
     1621 cgcacgctga gcggcaccga gtcgatccgc gagcggtgga agcgcggcgt ctcggtggtg
     1681 gagagcctga tgggtgacgc actgggcaag ctctacgtcg agcggcactt cccgcccggc
     1741 gccaaggccc gcatggacga actcgtcgcc aacctgcggg aggcccaccg ggtcagcatc
     1801 aaccagttgg agtggatgac cccggagacc cgtcagcgcg ccctggccaa gctcgacaag
     1861 ttcaccccga agatcggcta ccccccgaag tggcgggact actcgacact ggtcatcgaa
     1921 cgcgacgacc tctatggcaa ctaccgtcgc ggctatgcgc tgagctccga gcgagagttc
     1981 gccaagctgg gcggcccggt cgaccgcgac gaatggttca tgacgccgca gacggtgaac
     2041 gcctactaca acccgggcat gaacgagatc gtctttcccg cggcgattct gcagccgccg
     2101 ttcttcgacg ccgacgccga cgacgcggcc aactacggcg gtatcggggc ggtgatcggc
     2161 catgagatcg gccacggctt cgacgaccag ggcgccaagt acgacggcga cggcaacctg
     2221 gtggactggt ggaccgacga cgaccgcacc gagttcggcg cgcgtaccaa ggcgctgatc
     2281 gagcagtacg accagtacgt gccccgcgca ctgagcaacg ggtatcacgt gaacggtgca
     2341 ttcacggtcg gcgagaacat cggcgatctc ggcggcctgt cgatcgctct gctggcctac
     2401 cagctttcgc gcaacggtga gccggcaccc gtcatcgacg ggctgaccgg tgtgcagcgg
     2461 gtgttctacg gctgggcgca ggtgtggcgc acgaaatcac gtgacgccga gtcgattcgg
     2521 cggctcgcga tcgacccgca ctcgccgccg gagttccgtt gcaacggcgt gatccgcaac
     2581 atggacgcgt tttacgaggc cttcgaggtc gacgaggacg acgccctcta cctcgagccg
     2641 gagcgacggg tcaggatctg gagctgatct ccagcttgcc ctcgcccgcc tgaaagaacg
     2701 ggcgggtggc gagcacatag atcccgaaca ccgccagcgg cgcgagcagc ggcaaccacg
     2761 gcagctcgaa cgggaacgat gccgccagca cgccgatgaa cgtaaaccca agcgctgcaa
     2821 gcatcgccgt tgccgaggcg ccgaccaccc tggccgaacc accctcggtg tagcgcagca
     2881 ccaggtagca cgctgccgac aggccggcga gtgcggccgg catcggtggc gcgtcggaga
     2941 cgacgatcgc cgcggcggcc aacaggatcg ccggcgtcgc cgcaacgcga agcacgatcc
     3001 cggccaccac cgtgcccgcc gcgatcatcg cgagaaccag tgcgggcccg gaggtttgga
     3061 atcccgccgc tgccaccatc accaacccga aggccgtcga gagcaggtgg gctttccgaa
     3121 ggacgtccgt catcgtcgcc tccgcaccgg ccgccgatgc tcgggaacca gtcgcatcgc
     3181 ctggtcgagg gtgttctccg acggccacga cacgacgtcg acgccgacag tgcccatgtc
     3241 gcggtacatg aacgatctct gcatcatcca catccgggtg aacaacgggt cgatttcttc
     3301 tccgctcctc gcgtgcgcgg taccttttcc gctcctcgcg tgcgcggtac cttctccgaa
     3361 cggggcccct tcgaggacat cgaccgcgac gacggtgtgc ccgcgcttgc gcaggtcgat
     3421 cagcgagagt gcgaactcgg tgtcgagcat cgtggagaac gcgacgacga tcgcgcccgg
     3481 cggcaatgcg gcgcgcggcg ccaaactgcc tgtggtgggc tcgaattcgt caccggcacc
     3541 gagcagcgtg tcaagtaccc gatagaactg ccggcgcccg atgtcggcgc cgagccagcg
     3601 gggcctgccg ccgccgagcg cgacgacgcc ggcacggtca ccgctgcgca atgcgctctg
     3661 caccacctgc acggctccgc gcagcgcgcg ttcggtcgct tccgtcgccg ggccaggcgg
     3721 ctgggcgtac atgtcgatga gcaccaccac atcggcggcc cggtcggtca accgctgggt
     3781 gatgtgcagg ctgccgcgac gcgcgctcac cggccagttg acggtccgca attggtcacc
     3841 ggggacgtag ggccgaatgt cggcgtattc gacaccgggc ccgatgtgcc gagtcaggtg
     3901 cgtgccgagc cggtcgagca gttccgtctt gggaatggcg gtggactgtg gaggcgccaa
     3961 cgggaacaca aacgcgtcgg cgacgtcgac ggtcgcggtg cccgacagca gcccgccggg
     4021 cgcgacgacg tcgaccgtcg cgcggatcgg gtagcgaccc cacctgtcgg cggcggcggt
     4081 aaccgtctgc cgcccagagg gttcgggtat gacgtcgagt cgcataccgt cgacgccggt
     4141 gatccccagc gcgacggtga ggtcggcagg ctccgcggtg gcccacacgc tgacctgcgt
     4201 ctgctcaccc tcgaagcagc gctgcaggcc cggcgccgcg tgcacgaaga ccttgggcag
     4261 gccacgctgc cagccgatcg aacacaacac gccgagcagc ggcgccacga acgcgatcag
     4321 ctgccaccgc gacccgatca acgcggcgac gaggaccacg ccggcacagg tggccaccgc
     4381 gcgcgtcaac ggtgaggcgt gccagcgcaa gtcgacctcg gtgcgacggg tctcgatcac
     4441 gtacgggccc gcggaaccgg cagccgccgc aacagctcct ccacgacgtc ggcgccgtgc
     4501 acacggcgca cccacatctc cggcttcagg ctgatccggt gggccatggt
//
LOCUS       DASYLX010000990         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_723575,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000990 DASYLX010000000
VERSION     DASYLX010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723575"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGP27_23070"
     CDS             <1..634
                     /locus_tag="VGP27_23070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7854214.1"
                     /translation="LGGIGGEVLDIGCGLGDNAIFLASRGHSVTGLDGSPTAIEEARN
                     RAAQAGVSLTFGVADATDLTGYEGRFDTVVDSALYHCLDDDGRQAYAASLYRATRPGA
                     RWHLYCFSDGNVNGLVAPMGSVPESNLRDTLTANGWRIDFLGPTTCLASASGFGTGDE
                     DFPGAMAEQMPAEALEQMASVRARFSTIADLLDGDRVHLPFTAVHAHRVD"
     gene            780..1352
                     /locus_tag="VGP27_23075"
     CDS             780..1352
                     /locus_tag="VGP27_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEV7854215.1"
                     /translation="MNVHRILQSVEAVEALDGPADRSAAVASRLVGRGRFASLVRGSW
                     LGHPVHPLLVVLPLGAWISSVVLDVAFGDRTAARRLVGLGLVATPPTIIAGWADYPLL
                     TKRQRRVGIVHAASNAIGVAFFALSYRARRRERHRTATWLSVLGLLTVGVGGALGGHL
                     SYAQGAGVYRWQTARAIGHLELAERARAAA"
     gene            complement(1359..1586)
                     /locus_tag="VGP27_23080"
     CDS             complement(1359..1586)
                     /locus_tag="VGP27_23080"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEV7854216.1"
                     /translation="MSQNPAARPGRSVRRYGRTDVIHDATSLLMERFGLDAGQAVAQL
                     VKVSKRHSESIEAAARMCVDAQLRGDRLLAA"
     gene            complement(1697..2191)
                     /locus_tag="VGP27_23085"
     CDS             complement(1697..2191)
                     /locus_tag="VGP27_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854217.1"
                     /translation="MTMSTRVTAVAMSGLGVVGLGTGSAAAEVGDGGMVALSSVYRAC
                     DFTPSAYVNATGFGSGHVFIGTGGTDTVTADVHLAIGRPNTPYQVRLIQGPRPGSQKC
                     NAGDPGVVSGVLNTDGNGTGAVTIRDAQRPGATTAWVFIEGPPDFGQIRGEFYTSDVL
                     TKLK"
     gene            complement(2201..2566)
                     /locus_tag="VGP27_23090"
     CDS             complement(2201..2566)
                     /locus_tag="VGP27_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854218.1"
                     /translation="MPHDHDPVHWSNRPRTILAAGAAAVLLIGVLILLVMRTSGDSSA
                     PPDSFVPPSSSTPSSTYTTSTTTTTSYTTPSAQTSDQNLPPAGPPPPDDGGTSTPTTE
                     DTPNSGTVTETATLSPPPS"
     gene            complement(2602..3363)
                     /locus_tag="VGP27_23095"
     CDS             complement(2602..3363)
                     /locus_tag="VGP27_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV7854219.1"
                     /translation="MADKQVLEQRAAALLEMHRPGNPVVLPTVWDAWSAKLAVDAGFS
                     ALTVGSHPLADSIGKADGEVMTFDDVVARVSQITDAVDVPVSVDIESGYGEAPTRLIN
                     GLLDAGAVGLNIEDTVHKEGGRLRSSAEHAELVRALRAAADGAGVHVVINARTDLFLR
                     GDGDESDRVDRAIARLTEAADAGADVLYPVGRHDPDTLRRLASELPLPVNAIAYPDQS
                     DPASFGPLGVGRISFGPFLQAALSARAEELLQRWR"
BASE COUNT          527 a         1214 c         1210 g          590 t
ORIGIN      
        1 gctcggcggc atcggcggcg aggtgctcga catcggctgc ggcctcggcg acaacgcgat
       61 ctttctcgcg tcgcgcggac attcggtgac cggtctcgac ggctcgccca ctgcgatcga
      121 ggaggcccgc aaccgcgcag cgcaggccgg cgtctcgctg accttcggcg tcgccgacgc
      181 gaccgacctg accggctacg agggccggtt cgacaccgtc gtcgacagcg cgctctatca
      241 ctgcctcgac gacgacggcc gccaggccta cgccgcgagc ctgtaccgcg cgacgcgtcc
      301 cggtgcccga tggcatctgt actgcttctc cgacggaaac gtcaacggcc tcgtcgcgcc
      361 gatgggatcg gtgcctgagt caaaccttcg cgacacgttg acggcgaacg gctggcgcat
      421 cgatttcctc gggccgacaa cgtgtttggc gagtgcttct ggcttcggca ctggtgacga
      481 ggactttccg ggggcgatgg cggagcagat gcctgctgag gcgctggaac agatggccag
      541 cgtcagggcg cggttctcca ccatcgccga cctcctcgac ggcgaccggg tgcacctgcc
      601 gttcacggcc gtgcacgccc accgggtcga ctgacggcgg acctcacaca ggcccatcct
      661 ggccggtgag acggaattca cggcgcggcg agcggcgaca tgcccggggc cagactgatc
      721 gccagcaggt tccgccgaac acgctgattg acggtgccga gggcgggtac ctcgcagcga
      781 tgaacgttca ccggattttg cagagtgtgg aggcggtcga ggcgctcgat ggccccgcgg
      841 accgtagcgc cgccgtggcg agtcggctgg tcggcagagg taggttcgcg tcacttgtcc
      901 gcggctcgtg gctcgggcac ccggtccatc cgctgcttgt cgtgcttccg ctcggcgcct
      961 ggatcagctc cgtcgtcctc gacgtcgcct tcggcgaccg gaccgccgcc cggcggctgg
     1021 tcgggctcgg gctggtcgcg acgccgccga ccatcatcgc cggctgggcc gactacccgc
     1081 ttctgacgaa gcgccaacgg cgggtcggca tcgtgcacgc cgcgagcaac gccatcggtg
     1141 tggcgttttt cgcgttgtcg tatcgggcgc ggcggcgtga acggcaccgc accgcgacat
     1201 ggctgagcgt gctggggttg ctgacggtcg gcgttggcgg cgccctcggc gggcacctct
     1261 cgtatgcgca gggcgcgggc gtgtatcgct ggcaaaccgc ccgcgcgatc ggacatctcg
     1321 aactggccga gcgtgctcgc gcggccgctt agatacggtt acgccgcgag caggcggtcg
     1381 ccgcgcagtt gggcgtcgac gcacattctg gcggccgcct cgatcgactc gctgtgccgc
     1441 ttggacacct tcaccagctg agcgacggct tggcctgcgt cgaggccgaa ccgctccatg
     1501 agtagcgaag tcgcgtcgtg gatgacatcc gtccgcccat acctacgtac ggaccgcccg
     1561 ggccttgcgg cggggttctg ggacatgcgt gctggttacc acatagaacg cacgggcaaa
     1621 ccgccgcatc cgctatccgc agatttcgac gaagccgtcc gcgggacatc accgcggcac
     1681 ggtgttctcg ccgcggctac ttcagtttgg tgaggacgtc cgaggtatag aactcgcccc
     1741 ggatctggcc gaaatccggc ggtccctcga tgaacaccca cgcggtggtg gcacccggac
     1801 gttgggcgtc gcgaatcgtc accgcacccg ttccgttgcc gtcggtgttc agcacaccgc
     1861 tcacgacgcc cgggtcgccg gcgttgcatt tctgcgaccc gggccggggt ccctgaatca
     1921 gccgcacctg atagggggtg ttcggcctgc cgatggcaag gtgcacgtcg gcggtcaccg
     1981 tgtcggtgcc cccggtgccg atgaacacgt ggcccgaacc gaagcctgtc gcgttgacgt
     2041 acgcggatgg ggtgaaatcg cacgccctgt agacggagct gagcgcgacc atcccgccgt
     2101 cgcccacctc cgccgccgcc gagcctgtcc ccaacccgac cactcccagc ccggacatag
     2161 cgactgccgt gacgcgcgtc gacatagtca ttccgccagc ttacgacggc ggcggtgaca
     2221 gcgttgccgt ctcggtgacg gtgcccgaat tcggtgtgtc ctcggtcgtc ggcgtcgatg
     2281 tcccaccgtc atcgggcggc ggcggaccgg caggcggcag gttctggtcg ctggtctgcg
     2341 cgctcggcgt ggtgtaactg gtcgtcgtcg tggtcgacgt cgtgtaggta ctcgacggcg
     2401 tgctgctcga tggcgggacg aacgagtcgg gcggtgccga cgaatcgccg gaggtccgca
     2461 tgaccaggag tatcagcacc ccgatcagga gcaccgcggc cgcgcctgcg gccaatatcg
     2521 tccggggccg gttggaccag tgcacgggat catggtcatg aggcacgccg gaatgctagc
     2581 cgcagccgtc cgccagacgc tctacctcca gcgctgcagg agctcttcgg cacgcgccga
     2641 gagcgccgcc tgcaggaagg ggccgaagct gatccgtccg acgcccagtg ggccgaagga
     2701 cgcgggatcc gactgatcgg gatacgcgat ggcgttgacc ggaagcggca gttcggaggc
     2761 caggcgccgc aacgtatccg ggtcatggcg cccgaccgga tacaacacgt ctgcgcccgc
     2821 atcggcggct tcggtcagtc gcgcaatggc gcggtcgacg cggtcggact cgtcgccgtc
     2881 cccgcgaagg aagagatcgg tgcgcgcgtt gatcaccaca tgcacgcctg cgccgtcggc
     2941 cgcggcccgc agcgcgcgaa cgagttcggc atgctcggcc gacgaccgca gccgtccacc
     3001 ctccttgtgc accgtgtcct cgatgttcaa cccgaccgcc ccggcgtcga gcagtccgtt
     3061 gatcagccgc gtgggcgcct cgccgtagcc ggattcgatg tcgaccgaca ccggaacgtc
     3121 gaccgcgtcg gtgatctgcg aaacgcgggc gacgacgtcg tcgaatgtca tgacctcgcc
     3181 gtcggccttg ccgatcgagt cggcgagcgg gtggctgccg acggtcagcg ccgagaagcc
     3241 ggcgtcgacc gcgagcttcg ccgaccacgc gtcccacact gtgggcagca ccacgggatt
     3301 gccgggtcgg tgcatttcga gcagggcggc tgcccgctgc tcgaggacct gcttatccgc
     3361 catgtcgccc ttccgccgtg cacgtgacgg gtctcacgca ctatcaccat gatgtagcta
     3421 gcatcgactg tcgtactgtg attcccgaca cccttcagcc caaggaggtc tgatgtcgag
     3481 cactaccgaa tttgccgaac tgcacgagct catcggtgga ctccgccggt gcgtgacttc
     3541 g
//
LOCUS       DASYLX010000991        23353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1819422,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000991 DASYLX010000000
VERSION     DASYLX010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23353
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819422"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3462)
                     /locus_tag="VGP27_23100"
     CDS             complement(<1..3462)
                     /locus_tag="VGP27_23100"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEV7854220.1"
                     /translation="MSVELSPNRQPIAVVGVSALFPGSTDDAGFWRDILAGSDLIRDV
                     PESHWLLEDFYDEDPAAPDKTYARRGSFLDPIDFDALGWGVPPSTIPATDTAQLLALV
                     LAQQVLQDAAQGQFETMDRSRISVILGVTGATELVSTMAGRLQRPVWVKGLREAGIPE
                     DQVQAACERISANFVPWQEATFPGLLGNVVAGRIANRLNLGGTNCVTDAACASSLSAL
                     SMAVSELQLGNSDLAISGGVDALNDIFMFMCFSKTPALSPTGDCRPFSDNADGTMLGE
                     GLAMVALKRLADAERDGDRIYAVIRGIGAASDGRSKSVYAPVSEGQARALRRAYDLAG
                     YGPDTVELVEAHGTGTKAGDAAEFDGLRKVFEESGRTDRQWCALGSVKSQIGHTKAAA
                     GAAGLFKALMALHHKVLPPTIKVAAPNPNLRLPETPFYLSTKTRPWIRDSKHPRRASV
                     SSFGFGGSNFHVTLEEYTGPAPRAGRLRTAARELVLVCGASITEVAQQARDLAQCRDS
                     LDTIARRSQHTFDAGSPARLAVLASSPTDLAGKLVTAASRVEAQAEEFALPDGTAFGL
                     GPCESKVAFLFPGQGSQYLGMGADLAMGFEAARGPWDRAADLELEAGVPLHEVVFPRP
                     QFGVDAERLLTERLTATQWAQPAIACASVAQLAVLRGLAIDADMVGGHSFGEVTALFA
                     ADVLNESDLLRVARRRGELMAEAATVPGAMTALSLPVEKVQELLACQAPRLVVANHNG
                     PTEVVVSGPTDAVEELETKLEGEGITARRLPVATAFHSDLVSGSCAPFAEFLTAVDFA
                     PARIAVYSNALAARHDLSPDALRRALAAQIASPVRFMEMVHSMYAAGARTFVEVGPAS
                     VLTRFVERILGSQPHLAVSLDRKGKDDVDSLLLALARLAAAGVPMKLGELFAEYRERT
                     ESPSDAKLAVSLNGSNYGKLYPPPGGAKALPQPNPVRPATADQADLDHDGLAAVPLAS
                     PRLAPTVASTPVGVEPSLASPSPWIHAFLDSQRQTAEAHAVYQRAMAESYGSFLQTAE
                     TGFRGLTALLGGEAATADARSTDASVPQPLPTPVASPVEAPAAVPMPDIVATTLAVEV
                     MAVPIPVAVLAPTPVTVPASVDLHALMLAVVAEKTGYPAEMLNLDLDLEGDLGIDSIK
                     RVEILAAI"
     gene            complement(3485..5215)
                     /locus_tag="VGP27_23105"
     CDS             complement(3485..5215)
                     /locus_tag="VGP27_23105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HEV7854221.1"
                     /translation="MHGTGNITGRNCIVNIGKTSAVPSSVQSGNVAHRLGQTMLLSDV
                     SAREARWLPSDRPAAFTPAELVTAAQTVRTTAYVVQDEAGRRGLGLGGKLVDDSVPGL
                     PVLASLPPLYPEWLGDRSFCETHGTRFPYVTGAMANGIATTRLVAAIARNGCLGFFGA
                     AGLPLSRLEAALDELQRELNPLGLPWGSNLIHSPNEPSLEEAVVDLYIRRGVQRVEAS
                     AFMGLTTALVRYACTGLTTDRSGQIVRRHHVFAKVSREEVARYFLEPPPARILHSLVE
                     RGQLTENEAKLAGHLPLAEDITTEADSGGHTDNRPLTTLFPTILALRDKITATRAYTR
                     PVRVGAAGGLGTPSAVAAAFALGAAYVLTGSINQACLESGLDSSGKAMLAQAGMADVG
                     MAASADMFEMGVQVQVLRGSTLFAVRSRKLYQLYKSYPSLQAIPADEREKVETQMLRT
                     SFEGAWKSTRAFWTERDPREVQKAEADPRHQMALVFRSYLGLASRWAIAGQADRTSDY
                     QIWCGPAMGAFNAMAQGSFLESPENRSVGQVAKNLLEGAAVITRAQQLRTFGLAVPAA
                     GFHFRPRPFA"
     gene            complement(5365..7104)
                     /locus_tag="VGP27_23110"
     CDS             complement(5365..7104)
                     /locus_tag="VGP27_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854222.1"
                     /translation="MTTSSISAVLRERASLQPDDTAFTFLDYENDWDGVAESLTWLEV
                     YRRTLNVASELILCASPGDRAVILAPQGLDYIATFLGALQAGLIAVPLSVPQVGVHDE
                     RVRSVLRDTAPSVILTTSAVVGDVTEYVQPQPGHCVPSIVEVDLLDLKSPKRFDAALD
                     GCPDTAYLQYTSGSTREPTGVVVSHRNILANFEQKSSNYFVDHGRVGPADATTVSWLP
                     FYHDMGLIFGICAPVLWGCHTVLMSPVSFLQRPARWMQLLASNFRAWTGAPNFAFELA
                     TRKTSDADMAGLDLANVLGIISGSERVHPATLRRFTERFARFNLRESAIKPSYGLAEG
                     TAFVATRRPGNPPEFVHFDSEKLSAGHAERCRSGGGTALVSYGVPWTPMIRIVDRDIR
                     TECPAGTIGEIWVHGDNVAKGYWRKPEETQCTFNAQLVGPSPGTPAGPWLRTGDLGFS
                     CDGELFIMGRIKDLLIVYGRNHYPDDIEATIQEITGGRVAAISVPGDGTEKLVAIIEF
                     KKLGDTEEDAMHNLDLVKSEVTSAISQSHGLSVADLVLVARGSIPITTSGKVRRAACV
                     QRYRQGAFTRIDA"
     gene            7653..13952
                     /gene="pks2"
                     /locus_tag="VGP27_23115"
     CDS             7653..13952
                     /gene="pks2"
                     /locus_tag="VGP27_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016810361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolipid-1 biosynthesis
                     phthioceranic/hydroxyphthioceranic acid synthase"
                     /protein_id="HEV7854223.1"
                     /translation="MVEPPVTPVAVIGLACRLPGGIDSSEQLWDALLRGDDLVTEIPP
                     ERWDAEQYYDPHPGVPGRSVSKWGAFVDDIAGFDAEFFGLDEREAISIDPQHRLLLET
                     SWEAVEHAGLNPAAIAGSRAGVFIGLTHADYSLIAANAHALEGPYGLTANNFSLASGR
                     IAHTMGVHGPAATVDSACSSGLLAVHLACRSLHEGESDLALAGGASLMLEPRKFASGS
                     AQGMLSPTGRCHAFDVAADGLVYAEACAVVLLKRLPDALRDKDRILAVVRGTAANQGG
                     RTVDIGTPSTDAQVAVYRAALAAAGVDASTVGLVEAHGTGSPIGDPIEYGSLAKVYGS
                     DGPCVLASVKTNFGHAQSASGVLGLMKTILALQHGVVPRNLHFTGLPDEMARIETELF
                     VPQAVTPWPTNGRLPRRAAVSSYGLSGTNVHAIVEQAPAPESRENLDNSRTGTAMAPP
                     LLFPLSSTSADGLRRTSRRLAGWVAKHADGLASGDGNQSGNASGTRLVDLAYTLARRR
                     AHRPVRTVVMAGSVEELTERLGEVADGETPYQPTVGQDDRGPVWVFSGQGSQWAGMGA
                     RLLVTEPVFAAAVEEAEPLIAREAGFSVTGSMSAPVTVTGADRIQPTLFAIQVALAAA
                     MRSYGVCPAAVIGHSEGEVAAAVVAGALSLEDGVRVICRRSRLMSRIAGAGAMASVEL
                     PAQRVLSELAAGGVSDVALSVVSSPQSTVVGGTAQSVRDLVAGWEQRGVMARVLAVDV
                     ASHSPQVDPILADLADALADLTPMEPAVPYYSATLYDPRDRPAWDARYWVDNLRYTVR
                     FAAAVQAALQDGHRVFGELAPHPLLADAVEQTARNLDIPLASLAGMRSDSVQPRGLRG
                     LVADLHSAGAAVNFAVLYPYGRLVGAPLPTWTHRQLLLTRDGQDHEAHRAYTVSVHPL
                     LGPHVCLHEEPERHIWQGQAGTAALPWLTDHRIHNVAALPESAYCEMAVAAARAVLGE
                     ASEVRDLSFGPVLLLEEETPVSAVASVASPGVVDFIVETSQEDGHSRRAAAVLHALED
                     EDPAPSYDMPALLAAHPCRLEGAEIRNWFDTLGVQYGPAFAGLAAAHTANGHVGSVLA
                     EVALPGPIRSTQAAYGIHPALLDACLQSVVAYSRARGEDSGGLLLPMGVRRLRAYGRT
                     ANARYCYARVTGTGAAEVEADLDLLDERGTVLLTVQGLRLGTGVSESERRDRLLNERL
                     LTIQWQRRELSDVPPVDAGKWLLISTSDAADLLASELTDAVKKSGAEATTMRWSRHAD
                     HRVDTERLRAGAGESDGVVVLAGQGNGDSGDESAWRAREQVELLVGIARELSDIPGES
                     PRLYLVTRKAQSVLSDDLPDLQHAGLRGLMRVIGVEHPHLRATQIDVDDETDADQVAL
                     QLLNGSEEDETAWRDGEWFTARLCPGALRPDERRTTVVDHEHDGMRLRIRTPGDIESM
                     ELIAFDRIPPGPGQIEVAVTASDINFADVLVAFGRYPISDGRMPPLGTDFAGVVTAVG
                     PGLTEHRIGDHVGGLSSDGCWGTFVTSDARLAVTLPPGVPDERAAAVPTAYATAWYGL
                     HDLARISSRDKVLIHSATGGVGQAAMAIARAAGAEIFATAGSQQRRELLHGWGIERVY
                     DSRSIEFAEQIRRDTDGYGVDIVLNSVTGAAQRAGLELLSFGGRFIEIGKRNIHGDTR
                     LALFPFRRNLAFHGVDLALLTISHPERFRDLLATVYRQIADGALPLPECTHYPLRDAA
                     TAVRVMSAAEHTGKLVLDVPRIGHSTVVVPPERARVFRADGSYIVTGGLGGRGLFLAA
                     KLAEAGCGRIVLISRSAPKPEAREAIERIRGTGADIAMECGDISEPESADRAVAAAIA
                     TGLPVRGVLHAAAVVEDATLTNITDELVERIWAPKVYGAWNLHHATAGQPLDWFCSFS
                     SEAALVGTPGQGAYAAANSWLDAFTRWRRAQGLPAISIAWGAWAEIGCATVLAESTGT
                     AISPDEGAYAFETLLRHDRPYCGYAPIMGTPWLTALAQRSCFAEVFQSRADTTMFRAE
                     LNEVPREEWPTTLRRLVSEQISLILHRAIDPDRPLFEYGLDSLRNLELRTRIETRTGV
                     RISPTDITTVRALAGHLCEKLTAQA"
     gene            14002..15435
                     /locus_tag="VGP27_23120"
     CDS             14002..15435
                     /locus_tag="VGP27_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV7854224.1"
                     /translation="MGLIDEWVPTPGSVVSWHPSPAAIAKAQLAPISAVPASYMQAQH
                     LRGYSEQAARGLDYSRLVIATWTLAGRCDTRAMSYVINAHLRRHDTYRSWFEYKDADH
                     IVRRTLPDCSDIEFVPIDHGEMTAAELRRHIVSTPDPLHWDCFRFGVIQATDCFTVYV
                     SVDHLNMDATFVAVAHMEFYLMYASLVGGGAPVVLPEAGSYDDFCVREHQYLSTLTSE
                     SPLVRAWTEFAENNNGSFPDFPLPLGDPAVPCTAGMVTETLMKEHQMIRFESACVEAG
                     ARFVGGVLACAALAEHELTGSDTYYGLTPRDTRSAPADFTTAGWFTGLIPITVPIAPA
                     FGDTARAAQASFDSGTDLANVPFYRVLELAPRLTWPRPNYPVVNYFDAGVPPLSAFFT
                     ARLDATNVGVYTDDRYSYQMSIFVVRVEKETRVMVMFPNNEIARASVGRYVDAMKTVC
                     ARVAEGRAAVPPRSAAQSGIALGTIKC"
     gene            15569..18583
                     /locus_tag="VGP27_23125"
     CDS             15569..18583
                     /locus_tag="VGP27_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HEV7854225.1"
                     /translation="MRRLADFMVRWPWAVIGLWVAIAIALPLTLPSLNEMAQRHPLAI
                     LPSDAPSSVAARQMTEAFHESGTDNLLLVALINENALKPADEAAYRKVVDALRDDVTD
                     VVMLQDFVTTPQLRPFLTSEDKTTWVLPVGLAGELGTPRAYDAYNRVADIVKQNVVAD
                     GPLTVHVTGPAATVADLTVAGEQDRLPIEIAIGVLVLAVLLLVYRNPVTMLLPLAAIG
                     MSLLTAQGVVAGVSQLFGLGVSNQSIIFLTAMIAGAGTDYAVFLISRYHDYLRLGADL
                     DDAVRRTLISVGKVITASAATVGITFFGISFAKMGVFSTIGASSAIGIGVAYLAAMTL
                     LPAILVLAGPRGWVKPRRELTARFWRRSGIRIVRRPKAHLVGSLLVLALLASCATLVH
                     FNYDDRKALGPSASSSVGYAALERHFDVSQAMPEYILVKSPRDLRTPHALADLEQMAQ
                     RVSQLPNIGLVSGLTRPTGVVPQEFRATYQAGIVGDRMAAGSAAISENSKNLNRLTNG
                     AGTLAASLGDVRAQINRIAPSMQRLIDAFSSMRSQSGGDKLVKQVDTAAQLVQSINAL
                     GNAMGVNFRTVRDMFAWVGPVLAALDGNAICDANPSCSDTRMQFQKLVAAGDDGRLDQ
                     INTLAGQLQGFGDRQTLNATVKQLNDALSSFSSAVRSMGLDQPGGLEAGMTRVRQDAN
                     RLADGSQQVAGGVDQLVGQVKQMGAGLDESAAFLLTMKDDAGDPSMAGFNLPAQILQL
                     DEFKKAAKIFISPDGHSVRYLVQTKLDPFSTEAMDQVNAIGDTARGAQPNTTLSDASI
                     SMGGFPAALRDTRDYYEHDIRFIILVTFVVVLLTLMVLLRAIVAPLYLVVSVVISYFA
                     ALGISVLVFQYALGQQLHWSVPPLAFVVLVAVGADYNLLFVSRMRDESPHGMRYGVIR
                     TLTSTGGVITAAGLIFAASMSGLLFSSITTVVQGGFVIGVGVLLDTFLVRTVTVPAMA
                     TLVGRANWWPSRLSSQRPVRQPQSQSPAGQAE"
     gene            18596..19732
                     /gene="pe"
                     /locus_tag="VGP27_23130"
     CDS             18596..19732
                     /gene="pe"
                     /locus_tag="VGP27_23130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase PE"
                     /protein_id="HEV7854226.1"
                     /translation="MKRVLAFVTALTTFGVAGGFGFGIAAADEGPVTPTDPTPTDGPP
                     PLGTPGRGYALGGAHVLGIPYDEYIKRTGADWFPGLDRQIVDYPAGQVQGHTLERMFP
                     GIGRLDDNLPGLGVDGPSIGESVDEGEGNLEAAIRNGGPGTTIGLSEGAMVLNAVQAR
                     LANDPTAPPPDQLSFATYGDPVARHAFGESFLTQMFPVGSVVPSLDYRIPPPVESQYD
                     TYHFVSAYDSIADWPDRPDNWIALANAVVGLATGHTAVAFTDPSMVPPQNIRTTVNSR
                     GAKTTTYMIPEQHLPLVLPFKYFGVDEGTLNQLDGVLKPMVDAAYSRNDDPLTAPITV
                     DPVRGYDPATVTAPATQAAFGGAATDPVSQLLAGFQYVLNHQQP"
     gene            20049..21359
                     /locus_tag="VGP27_23135"
     CDS             20049..21359
                     /locus_tag="VGP27_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854227.1"
                     /translation="MSRPGADSHTTSRSRVVAWALWDCGATGVNAVVVTFVFSVYLTE
                     QVGSGLPGPATPASWLGRVLTIAGLCVALLAPATGIWVNAPNRRRPALALLTGVVVAM
                     TGAMSLIRADYHYLWPGLVLLALTAACSDLATVPYNAMLRQLSTGETSGRISGLGSAA
                     GYVGSVLLLLIIYFGFIQGDGDTRGLLGLAAVDGGNVRAAMLLAAAWLALFAWPLLVC
                     VRTPAFVDPPPTVGFLGAYRALWDEVKGEWRRDRNVVYYLLASAVFRDGLTGVFAFGA
                     VLGVSVYGVSQADVLVFGVCASIVAAVGAVLGGLLDDRVGSKPVIVGSLAAMIAVGLL
                     LLTLSGPLAFWICGLLLCMFVGPILASARALMLRMSAEGKEGVAFGLYTTTGRAVSFL
                     APWMFFTFIDLFGTGRAGLGGLCVVLVAGLLAMMIAVRVPREAN"
     gene            complement(21462..22904)
                     /locus_tag="VGP27_23140"
     CDS             complement(21462..22904)
                     /locus_tag="VGP27_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854228.1"
                     /translation="MAGHPLCRRIAAVLDLAPDASAIEYDGQWFTYGQIGVLARRVAT
                     MAAGRGQVGMLLRNRPTHVAAFLGVLLAGGSVVVINPSRGDDRTKADIAALDLPLIIG
                     DPDDLTKLVGPSAGSTVISLSVLDAEPQLVAVGDGHRATRSGVAVRMLTSGTTGPPKR
                     IDLTYDMLAHSVMGRDPGRSPSRSEVRGGVAIVNAPLVHIGGVFRVLQCVAEARPFAL
                     LDRFELGTWVDAVRRHRPRAVSLVPAALRMVLHSAVRPEDLESIRAVTSGTAPLSAED
                     ADAFQQKFGIPVLTSYAATEFGGGVAGWTLADHQKYWQAKRGSVGRANPGAELRVVDD
                     DGKPLGADQPGLLQVKPGQLGPSADWMRTTDMARIDEDGFLWILGRADQAIIRGGFKV
                     MPDDVRAALESHPAVQGAAVVGRPDGRLGETPVAVVELRDAASADASELLEFLRNRLA
                     RYEIPTEIRIVDAIPRTPSGKPDLSAIRGG"
     gene            complement(22904..>23353)
                     /locus_tag="VGP27_23145"
     CDS             complement(22904..>23353)
                     /locus_tag="VGP27_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7854229.1"
                     /translation="GAFYFVNESDVVICSQDATFFDSHVSAGLVCALEPIGLMRRVGL
                     GETLRIALMGNDERVSADTALRIGLVSEVVLRDRLWERAHAIAATIAAKPPSATQGTV
                     KAIWESLDKPYRAALEQNLIYTRLGNPIGAAELAQRGGPVKSEPRIR"
BASE COUNT         3866 a         7550 c         7830 g         4107 t
ORIGIN      
        1 gatggcggcc aggatttcga cccgtttgat cgagtcgatc ccgaggtcgc cttcgaggtc
       61 caggtcgagg ttgagcatct cggcgggata gccggtcttt tcggccacca cggccagcat
      121 cagcgcgtgc aggtcgacgg acgcaggcac agtgaccggc gtgggtgcga gcacggcaac
      181 cggtatcggc acagccatga cctcgacggc gagtgtagtg gccacgatgt cgggcatcgg
      241 cacggcggcc ggggcttcga ccggcgaggc caccggcgtc gggagtggct gcggaacaga
      301 agcgtctgtg ctcctcgcgt ccgcagtggc cgcctcgccg cccaggagcg cggtcagacc
      361 gcggaagccg gtctcagcgg tctgcaggaa agaaccgtag ctctcggcca tcgcccgctg
      421 gtagacggcg tgagcctcgg ccgtctgccg ctggctgtcc agaaatgcgt ggatccacgg
      481 cgacgggctg gcgagcgacg gctccacccc gaccggcgtg gacgcgacag ttggggcgag
      541 ccgcggggac gcgagtggca ccgcggcaag gccatcgtgg tccagatctg cctgatccgc
      601 cgttgccggc cgcacggggt tcggctgtgg caacgccttt gcaccgccgg gcggcgggta
      661 cagcttcccg tagttgctgc cattgagcga cacggcgagc ttggcgtccg acgggctttc
      721 ggtgcgctct cggtattcgg cgaacagctc gccgagtttc atcggcacac ccgccgcggc
      781 cagccgggcg agcgccagca acaacgaatc gacgtcgtcc ttgcccttgc ggtcgaggct
      841 gactgcgaga tgcggttggc tgccgaggat ccgctccacg aagcgggtca gcactgatgc
      901 cggacccacc tcgacgaacg tccgggctcc ggccgcatac atcgagtgca ccatctccat
      961 aaagcggacg gggctcgcga tctgcgcggc cagtgccctg cgcaaggcat cgggcgagag
     1021 gtcgtgccgg gcggccagcg cgtttgaata gaccgcaata cgggccggcg cgaagtcgac
     1081 tgcggtcagg aattcagcga acggcgcgca cgaaccagac accaaatccg agtggaaggc
     1141 ggtggccacc gggagacgac gggctgttat gccctcgccc tcgagcttcg tctcgagctc
     1201 ctcaaccgcg tcggtggggc cggagacgac gacctcggtc gggccgttgt ggttcgcgac
     1261 gaccaatcgc ggcgcctggc acgccagcag ttcctggacc ttctccacgg gtaaggaaag
     1321 agccgtcatg gcaccaggca ccgtggccgc ctcggccatc agctcgccgc ggcggcgggc
     1381 cactctcagg agatcgcttt cgttcagcac gtcggccgcg aacagcgcgg tcacttcgcc
     1441 gaagctgtgg ccgcccacca tgtcggcatc gatggcaagt ccgcgcagca cggcgagctg
     1501 ggcaacagag gcacaggcga tcgcgggctg tgcccactgg gttgccgtga ggcgctcggt
     1561 gagcaaccgc tcggcgtcga ctccgaactg tggacgcggg aacaccacct cgtgtagcgg
     1621 cacgcccgcc tccaactcca gatccgcggc gcgatcccag ggaccgcggg cggcttcgaa
     1681 gcccatcgcc aggtcggcgc ccatgccgag gtactggctg ccctggcccg ggaacaggaa
     1741 ggcgaccttg ctctcgcacg ggcccagccc gaacgcggtg ccgtcgggca gcgcgaactc
     1801 ctcggcctgc gcctccacgc gggaagcggc ggtgacgagc ttgccggcaa ggtcggtggg
     1861 gctggatgcg agcaccgcca gacgggcagg cgagcccgcg tcgaaggtgt gctggctgcg
     1921 ccgggcgatg gtgtcgaggg agtcgcggca ctgagccaga tcgcgggcct gctgcgccac
     1981 ttcggtgatg gacgcaccgc acaccagcac cagctcgcga gcagccgtcc gcagccgccc
     2041 ggcgcgcgga gcgggacccg tgtactcctc caacgtcacg tggaagttgg acccaccgaa
     2101 gccgaaggag ctgactgacg cgcgccgggg gtgcttgctg tcgcggatcc agggccgcgt
     2161 cttcgtcgac aggtagaacg gcgtctcggg cagtcgcaga ttgggattgg gcgcggcgac
     2221 cttgatggtc ggcggcagca ccttgtggtg cagtgccatc agagccttga agaggccggc
     2281 tgcgccggcg gcggccttgg tgtggccgat ctggctcttg accgagccaa gcgcgcacca
     2341 ctgtcgatcg gtccggccgc tctcctcaaa gaccttgcgc aggccgtcga attcggcggc
     2401 gtcgcctgcc ttcgtgccgg tgccgtgggc ttcgaccagc tcgaccgtgt ccggcccgta
     2461 gccggccaga tcgtaggctc ggcgcagggc gcgggcctgg ccctcggaga ccggcgcgta
     2521 gacactcttc gatcggccgt ccgaggcggc accgatgccg cgaatgacgg cgtagatgcg
     2581 gtcgccgtcg cgctctgcgt cggcgaggcg tttgagcgcg accatggcca ggccctcgcc
     2641 cagcatggtg ccgtcggcat tgtcgctgaa gggccggcag tcgccggtgg gggagagcgc
     2701 cggcgtcttc gaaaagcaca tgaacatgaa gatgtcgttg agcgcgtcga ccccgcccga
     2761 tatcgccagg tcgctgttgc ccagttgcag ttcggacacc gccatggaca gggcgctgag
     2821 cgaggaggcg caggccgcgt cggtcacgca gtttgtgccg cccaggttca gccggttggc
     2881 gatccggccg gccaccacgt tgccgagcag tcccgggaac gtcgcctcct gccacgggac
     2941 gaagttggcg gagatccgct cgcacgcggc ctgcacctga tcctcaggaa ttccggcctc
     3001 ccgcagcccc ttcacccaga ccgggcgctg taggcgacca gccatggtgg aaaccagttc
     3061 cgttgccccg gtgacgccga ggatcaccga tatgcgggag cggtccatgg tttcgaattg
     3121 gccctgcgcc gcgtcctgca gcacctgctg ggccaagacc agggccaaca gctgggcggt
     3181 gtcggtggcg gggatcgtcg aggggggaac cccccacccg agcgcgtcga agtcgatcgg
     3241 gtcgaggaag gagccgcggc gcgcgtacgt cttgtcaggg gcggcgggat cctcgtcgta
     3301 gaaatcctcg agcagccagt ggctttccgg aacgtcgcgg atcaggtccg atccggcgag
     3361 aatgtctcgc cagaacccgg cgtcgtcggt ggagccgggg aagagggcgc tcaccccgac
     3421 caccgcgatg ggctgacggt tgggcgatag ttcgacggac atgcttactc ccacgaaggt
     3481 tcagtcaggc aaatggacgg ggccggaagt ggaagccggc cgcagggacg gccagcccga
     3541 aggtgcggag ttgctgcgca cgggtgatca cagcggcacc ttcaaggagg tttttcgcca
     3601 cctgcccgac gctccggttc tcaggtgact cgaggaagct gccctgggcc atggcgttga
     3661 acgcgcccat ggccgggccg caccagatct gatagtcgct ggtgcggtcg gcctgtccgg
     3721 cgatggccca gcggctggcc agacccaggt aggagcggaa gacgagcgcc atctggtgtc
     3781 tgggatcggc ctctgccttt tgcacttcgc gagggtcgcg ctcggtccag aacgcccgtg
     3841 tcgatttcca ggccccctcg aaactggttc gcagcatctg ggtctcgacc ttctcgcgct
     3901 cgtccgccgg aatggcctgc agcgaggggt acgacttata caactgatag agcttccggc
     3961 tgcgcaccgc gaacaacgtg ctgccgcgca gtacctggac ctggacgccc atctcgaaca
     4021 tgtcagcgga cgcggccatc cccacgtcag ccataccggc ctgggccagc atcgcctttc
     4081 ctgacgaatc taggccgctc tccaggcagg cctgattgat ggagccggtg agcacgtagg
     4141 cggcgccgag cgcgaaggcc gcggcgaccg ccgacggggt acccagaccg cccgccgcgc
     4201 cgacgcgtac cggcctggtg taggcgcgcg tcgccgtgat cttgtcgcgg agcgccagga
     4261 tggtcgggaa cagcgtggtc agcggtcggt tgtcggtgtg gccgccggag tcggcctcgg
     4321 tggtgatgtc ctcggcgagc ggtaagtgcc cggcgagctt cgcttcgttc tcggtgagct
     4381 gcccgcgctc caccaggcta tgcaggatcc tggccggagg cggctccagg aagtagcgcg
     4441 ccacctcttc acgggagacc ttcgcgaaca cgtggtgcct gcgcacgatt tggcccgatc
     4501 ggtcggtggt gagcccggtg cacgcgtatc gcaccagggc cgtggtcagg cccatgaagg
     4561 ccgaggcctc gacgcgctgt acgccacggc ggatatagag gtcgaccacg gcctcttcca
     4621 gcgacggctc gttcgggctg tggatcaggt tcgagcccca gggaagaccc agcgggttca
     4681 attcgcgctg caattcgtcg agcgcagctt ccagtctgga gagcgggaga ccggcggccc
     4741 cgaagaagcc caggcagccg ttgcgggcga ttgcggctac cagccgagtg gtggcgatgc
     4801 cgttcgccat ggcgccggtc acgtagggga agcgggtgcc gtgggtctcg cagaacgagc
     4861 gatcgccaag ccattccggg tagagcggcg gcagcgaggc gagcaccggt agccccggca
     4921 cggagtcgtc cacaagcttg ccccccaatc ccaagccgcg ccggcccgcc tcgtcctgga
     4981 ccacgtaggc cgtggttcgc accgtctggg cggcggtcac caactcggcg ggcgtgaatg
     5041 cagccggccg atcggaggga agccaccgag cctctcgcgc tgaaacgtca ctcagcaaca
     5101 ttgtctgccc caatcggtgt gcgacattgc cgctctgaac gctactcgga acggcggaag
     5161 ttttaccgat attgacgatg cagttacggc cggtaatgtt gcccgtgccg tgcatggcat
     5221 cggcaaatcc ttgagctagc aacaactttg acgacacaag acgttgcgcc gtgctgcacc
     5281 gcggcgcgac cacgcgtgcc ggtgggcagt gtcagcccgc acgtcacccg gccggacgac
     5341 acgcgatgct ggctatgtgc ccgcctaggc gtctatgcgt gtaaatgcac cctgtctgta
     5401 ccgctggaca caggcggctc gtctgacctt gccgcttgtc gttatcggaa tcgatccgcg
     5461 cgccaccaga acaagatccg cgacgctcag accgtgtgat tgtgagatcg ccgaggtgac
     5521 ttcactcttg accaagtcga ggttgtgcat ggcatcttcc tcggtgtcgc ctagcttctt
     5581 gaattcgatg atggcgacga gcttctccgt gccatcacct gggacggaga tcgccgccac
     5641 ccggcctcct gttatctcct gaatagtcgc ctcgatgtcg tcggggtagt ggttacgccc
     5701 gtagacgatc aggagatcct tgatccggcc catgatgaac agttcgccgt cacagctgaa
     5761 gcccaagtct ccagttctca gccaggggcc tgccggcgtg ccaggtgagg gcccgacgag
     5821 ctgtgcgttg aacgtgcact gtgtctcttc gggcttccgc cagtaaccct tggcgacgtt
     5881 gtccccgtgc acccagattt caccgatggt tcccgctgga cattcggttc tgatgtcgcg
     5941 atcaacgatc cgtatcatcg gtgtccacgg aacgccatag ctgaccagag ccgtaccgcc
     6001 tccacttctg caccgctccg cgtggccggc cgacagtttc tccgaatcga agtggacgaa
     6061 ttctggtggg tttcccggcc tgcgggtcgc gacgaatgcc gttccttctg cgagtccata
     6121 tgagggcttt attgccgatt cgcgaagatt gaagcgcgcg aaccgctcgg tgaagcgtcg
     6181 cagcgtcgcg gggtgtaccc gttcgctacc gctgatgatg cccagcacat ttgcgagatc
     6241 cagtccggcc atgtcggcat ccgatgtctt tcgggtagcc aattcgaaag cgaagttcgg
     6301 tgctcccgtc catgcgcgaa aattgctggc caacaattgc atccacctgg ccggccgctg
     6361 gaggaacgac accggactca tgagcacggt gtggcatccc cacagaacgg gtgcgcaaat
     6421 tccaaaaatc aatcccatat cgtgatagaa cggcagccac gacaccgtcg tggcgtctgc
     6481 cgggccaact cttccgtgat ccacgaaata attggacgac ttctgctcga agttggccag
     6541 aatgttcctg tgcgacacca caactccggt cggctctcgc gtcgaccctg aggtgtattg
     6601 cagatacgcg gtgtcagggc aaccatcaag cgcggcgtcg aagcgtttcg gtgacttcag
     6661 gtccagcaaa tcgacttcga cgattgacgg aacacaatgt cccggctgcg gctgaacgta
     6721 ctcggtgacg tcgccaacga cagcggacgt ggtcagaatg acactgggcg ccgtatcgcg
     6781 cagcaccgaa cgaacacgct catcgtggac accgacctgt ggcaccgaaa gcggaactgc
     6841 aataagtccg gcctgcaatg cccctagaaa cgtcgcgatg tagtcaagcc cctgtggagc
     6901 caatatcact gcccggtcac cgggggacgc gcaaagtatc agctccgacg ccacgttcag
     6961 cgttcgccga tacacctcca accacgtcag gctttcggca acgccatccc aatcgttttc
     7021 gtaatcaagg aatgtgaacg ctgtgtcatc gggttgcaga ctcgcacgct cgcgtagcac
     7081 agcagaaatg gaagacgtag tcatggcggc gtgtacctcc cttgagatgt ccctgccgat
     7141 ctgtcaccgc cgggaagcaa gtacttccct tgtgtaagaa cttgtttgac atcgtggcgg
     7201 ttcgggtgag aaatgaggca gtgagtgccc gcgtggtgaa cggaagtgag cttaggcaca
     7261 tccagcacgc agtgtgcgct tacggcaaaa gtcggtgaga ctccgtgata tttcaaaggt
     7321 gtccatcggc ctgaatgttg ttgcagatat tgaaaaggca agctttcggc gccgtcgccg
     7381 accgcttgtg tggccttgtc gactgtctcg cgatgtgggc gtacatcgcg gcgtcaggtt
     7441 cggttgttga cctgaggtag gtcacggcac ggaattgggc agttgcgaat cgccattatc
     7501 tgcccagtca tgacgcagag cgatacgctg atgcgcacta ccctcgcttg gctgcgcgaa
     7561 agaacggttg acagacctgg ggcggaacct ccttcccctg agatgaatgc aattggcttt
     7621 cagcatcacc gggggaaaag aggtgaacgg acatggtcga accacctgtc actcccgttg
     7681 cagtcatcgg actggcctgc cgacttccgg gcggcatcga ctcgtcggaa cagctgtggg
     7741 acgcgttgct gcgtggcgac gatctggtca ccgagatccc cccggagcga tgggacgccg
     7801 agcagtatta cgacccccac ccgggtgtgc ccggccgttc ggtgtcgaag tggggtgcgt
     7861 tcgtagacga catcgccggc ttcgatgccg agttcttcgg tctcgatgag cgtgaggcga
     7921 tctcgatcga tccccagcac cggttgttgc tcgaaacctc ttgggaggcc gttgaacatg
     7981 caggactgaa tccggccgcg atagccggct cgcgcgccgg cgtcttcatt ggattgactc
     8041 acgccgacta ttcgctgata gccgccaacg cgcacgccct ggaggggccg tacggcctga
     8101 ccgccaacaa tttcagcttg gcgtccggac gcatcgccca cacgatgggg gtgcacggtc
     8161 ccgcggccac ggtggactcg gcatgttcgt ccggcctgct cgccgtccat ctggcatgtc
     8221 gcagcctgca cgagggcgaa agcgatctcg cgctcgccgg aggagcctcg ctgatgctgg
     8281 aaccgcggaa gttcgcctcc ggatccgcgc agggcatgct ctctccgacc gggcgctgtc
     8341 atgcgttcga cgtcgcggcg gacgggttgg tgtatgccga ggcttgtgca gtggtgttgc
     8401 tcaagcggtt gccggatgcg ttgcgggaca aggaccggat cctggctgtc gtgcgcggta
     8461 cggcggctaa tcagggtggc cgtaccgtgg acatcgggac gccttcgaca gacgcacaag
     8521 tcgccgttta ccgggcagcg ctggcagcgg cgggtgtgga cgccagcacg gtcggcctgg
     8581 tggaggctca cggcaccggt agcccgatcg gtgacccgat cgaatacggg agcctggcca
     8641 aggtgtacgg cagcgacggc ccctgcgtgc tcgcgtctgt gaagacgaat ttcggacacg
     8701 cccagtccgc gtccggagtg ctggggctga tgaagacgat cctcgcgctg cagcacggtg
     8761 tggttccgcg gaatcttcac ttcaccggtt tgcccgatga aatggcccga atcgagacgg
     8821 aactgtttgt gccgcaagcg gtcacgccat ggcccacaaa cggccggctg ccacgacgcg
     8881 ccgcggtgtc ctcgtacgga ttgtcgggaa ccaacgtcca cgcgattgtt gagcaagccc
     8941 cggcgccgga gagccgggag aacctggaca actcgcgaac cgggacagcg atggcaccgc
     9001 cgctgctgtt cccgctgtcg tcgacctctg ccgacgggtt acgccggacc tctcggcggt
     9061 tggctggctg ggtggcgaag catgcggacg gccttgcaag cggagacgga aaccaaagcg
     9121 ggaacgcaag cggcacaagg ctggtggatc tggcctacac ccttgcgcgt cggagagcgc
     9181 accggccggt gcggacggtg gtgatggccg gcagcgtcga ggagctgacg gagagattgg
     9241 gcgaggtggc cgacggtgag actccgtatc agcccaccgt cggtcaggac gaccgcggac
     9301 cggtgtgggt gttctccggg cagggttcgc aatgggccgg gatgggcgcc cgcctgctcg
     9361 tcacggaacc ggtcttcgcc gcagccgtcg aggaggcgga gccgctcatc gcccgcgagg
     9421 ccgggttctc ggtgaccggg tcgatgtcgg cgcccgtgac ggtgaccggt gctgaccgaa
     9481 tccaaccgac cctgtttgcg atacaggtgg cactggccgc ggcgatgcgc tcgtacgggg
     9541 tgtgcccagc ggcagtcatc gggcactcgg agggcgaggt cgcggcggct gtcgtggcgg
     9601 gagcgctgtc gctggaagac ggcgtgcgcg taatctgccg gcggtcgcgg ctgatgtccc
     9661 gtatcgccgg tgccggcgcg atggcatcgg tggaactgcc cgcccagcgg gtgctgtcgg
     9721 agctggccgc aggcggcgtg agcgatgtcg ccctgtccgt ggtctcctca ccgcagtcga
     9781 ctgtcgtcgg cggcaccgcc caatcggttc gcgacttggt ggcaggctgg gagcagcgtg
     9841 gcgtcatggc gcgggtgctc gccgtagacg tcgcttcgca ttcgccgcag gtcgatccga
     9901 tcctcgccga cttggccgac gctctggccg atctcacccc gatggaaccc gcggtcccgt
     9961 actactccgc aaccctgtat gacccccgcg accgtccggc ttgggatgcc cgttactggg
    10021 tggacaatct gcgatacacg gtgcggttcg ccgcggcggt gcaggcggcg ctgcaggacg
    10081 gccaccgagt cttcggggaa ctggcgccgc accccctgct ggccgacgcc gtcgagcaga
    10141 ccgctcgcaa cctcgacata ccgctggcct cgcttgcggg catgcggagc gactcggtgc
    10201 agccgcgggg actgcgcggc ttggtggcgg atctgcacag cgccggcgct gcggtgaact
    10261 ttgccgtgct gtacccctat gggcgcctgg tgggcgcgcc gctgccgacc tggacccacc
    10321 gtcagctgct cttgacccgc gatggtcagg atcacgaggc gcatcgcgca tacaccgtct
    10381 cggtgcatcc gctgttgggg ccgcatgtgt gcttgcacga ggagccggag cgccacattt
    10441 ggcaggggca ggccggcacc gcggccctgc catggttgac cgatcaccgg atacacaacg
    10501 tggcagcgct gcccgagtcc gcgtactgcg agatggcagt cgctgcggct cgtgcggtgc
    10561 tcggcgaggc atccgaggtc cgcgacctct ccttcgggcc ggtcctgttg ctcgaggaag
    10621 agaccccggt ctccgccgtc gcatctgttg cgtcgcccgg cgtcgtcgac ttcatcgtcg
    10681 agacctcgca ggaggacggg cacagccgcc gggccgctgc ggtcctacac gcccttgaag
    10741 atgaggaccc ggcaccgtcc tacgacatgc ccgccctgct cgcggctcac ccgtgccgcc
    10801 tggaaggggc ggagatccgc aactggttcg acaccctcgg cgttcagtac ggaccggcgt
    10861 tcgcaggcct cgccgcagcg cacaccgcga acggacacgt cgggtccgtg ctggccgaag
    10921 tcgcgctgcc cgggcccatc cgctcaacac aggctgccta cggcattcac cctgcgctgc
    10981 tggatgcatg tctccagtcg gtcgtggctt actcccgcgc acggggtgaa gacagcggag
    11041 gtctgctgct gcccatgggt gtgcgtcggc tgcgggccta cggccgtacc gcaaacgccc
    11101 gctactgcta tgcgcgggtg accggtaccg gcgccgcgga ggtcgaggcc gacctcgatc
    11161 tgctggacga gcgcggcaca gtgctgctga ccgtgcaagg cctgcggctg ggaaccgggg
    11221 tctccgagag cgaacgacga gatcgtctgc tgaacgagcg tctcctgacc atccaatggc
    11281 agcggcgaga gctgtccgac gtcccgcccg tcgacgcggg aaagtggctt ctgatcagca
    11341 cttcagatgc tgccgatctg ctggcaagcg agttgaccga tgcggtgaag aagtccggcg
    11401 cggaagccac gaccatgcga tggtcacggc acgccgatca tcgggtcgac actgaacggc
    11461 tgcgtgccgg cgccggcgaa tccgacggtg tcgtagtgct tgcggggcaa ggaaacggcg
    11521 actccgggga cgagtctgcg tggcgagccc gggagcaggt agagctactc gtcgggatcg
    11581 cacgggagct ttcggacatc ccgggcgagt cgccgcgcct gtatctggtg acgaggaaag
    11641 cccaatcggt gctgtccgac gacctgcccg acctgcagca cgctgggctg cgtggcctga
    11701 tgcgagtgat cggcgtcgaa cacccccatt tgcgcgctac ccagatcgac gtagacgacg
    11761 agacggatgc cgatcaggtg gcgctgcaac tgctgaatgg atcagaggag gacgagaccg
    11821 cctggcgaga cggcgagtgg tttaccgcac ggttgtgccc cggtgcgctg cgccccgacg
    11881 agcggcgaac caccgtcgtg gaccacgagc acgacggaat gcgcctgcgc atccgcacgc
    11941 ctggtgacat cgaatcgatg gaactcattg cgttcgaccg cattccgccg gggcccgggc
    12001 agatcgaggt cgcggtcacc gcctccgaca tcaacttcgc ggatgtgctc gtcgccttcg
    12061 gccgataccc catctccgat gggcggatgc cgccgctggg gacagatttc gccggcgtgg
    12121 tgaccgcggt cggaccagga ttgaccgagc atcggatcgg tgaccatgtc ggcgggctgt
    12181 cgtccgacgg gtgttggggc accttcgtca cctccgacgc ccgcctcgcg gtcacgcttc
    12241 caccaggggt accggatgag cgggcggccg cggtaccgac cgcgtacgcc actgcctggt
    12301 acggcctaca tgatctggcc cggatctcct cgcgcgacaa ggtgttgatc cactccgcaa
    12361 ccggcggtgt ggggcaggcc gcaatggcga tagcgcgtgc cgcgggggcc gagatattcg
    12421 cgacggccgg cagccagcag cgtcgagagc tactgcacgg ctggggtatt gagcgcgtct
    12481 acgactcgcg cagcatcgag ttcgccgaac agatccgccg tgacaccgac ggttatggcg
    12541 ttgacatcgt gctcaattcg gtcactggcg cagcgcaacg cgccggtttg gaactgctgt
    12601 ccttcggggg acggttcatc gagatcggca agcgaaatat tcacggcgac actcgcttgg
    12661 cgctcttccc gttccgtcgc aacctcgcgt tccatggcgt cgacctggcg ttgctgacca
    12721 tcagccaccc ggagcgcttc cgggatctgc tggccacggt atacaggcag atcgcagacg
    12781 gtgccctgcc gctgccggaa tgcacgcatt acccgcttcg cgacgcggcc accgccgtcc
    12841 gagtgatgag cgcggccgag cacaccggga aactcgtgct cgacgttccc cgcatcgggc
    12901 acagcaccgt ggtggtgccg ccggagcggg cgcgggtctt ccgcgccgac ggctcctaca
    12961 tcgtcaccgg cggcctgggt ggccgtggcc tcttcctcgc ggccaagttg gccgaggcgg
    13021 gctgcgggcg gatcgtgctc atctcgcgct ctgcgcccaa acctgaagcg cgcgaggcca
    13081 ttgagcgcat ccgcggcacg ggagccgaca tcgcgatgga gtgtggcgac atctccgaac
    13141 ccgaatcggc ggaccgcgcg gtggcagcgg cgatcgccac cgggcttccc gtgcgggggg
    13201 tgctgcacgc ggcggcggtg gtcgaggacg ccacattgac caacatcacc gatgaactcg
    13261 tcgaacgcat ctgggcgcca aaggtatacg gtgcctggaa cctgcatcac gccacggccg
    13321 ggcagccact ggactggttc tgctcgtttt cctcggaagc cgccctggtg ggcacgccgg
    13381 gacagggggc atacgccgca gccaacagct ggctggacgc cttcactcga tggcgccggg
    13441 ctcaaggcct tccggccatc tcgattgcgt ggggagcatg ggcagagatc ggctgcgcga
    13501 ctgtcctggc cgaaagtacc ggcaccgcca tcagtcccga tgagggcgcc tacgcgttcg
    13561 agacgctgct gcgccacgac cgcccgtact gcggctatgc gccgataatg ggaacaccgt
    13621 ggttgacggc cttggcgcaa cgcagctgct tcgctgaagt gttccaaagc cgtgcggaca
    13681 caacaatgtt ccgcgccgag ctgaacgaag tgccccggga ggagtggccg accacgctcc
    13741 gccgtctggt gtccgagcag atcagcttga ttctgcatcg cgccatcgat ccggaccgtc
    13801 cgctgttcga gtacgggctc gactcgctgc gcaacctcga actgcgcacc cgcatcgaaa
    13861 ctcgaacggg cgtgcgcatc tcacccacgg acatcaccac cgttcgtgca ttggcggggc
    13921 acctgtgcga aaagttaacg gcccaggcct gaaccaggcg tggagaagcg aggagagaaa
    13981 gtgatcatcg gagcggtggc aatgggattg atcgacgagt gggtgccgac tccaggttca
    14041 gtcgtatctt ggcatccgtc acccgccgcg atagcgaaag cacagctagc accgataagc
    14101 gcggtaccgg ccagctatat gcaagcccaa caccttcgtg gctacagcga acaagcagcc
    14161 cgcggactcg actattcacg cctggtcatc gccacgtgga ctcttgccgg ccgctgcgac
    14221 acccgtgcca tgagttacgt catcaacgcg caccttcgtc ggcacgacac gtaccgcagt
    14281 tggttcgagt acaaggatgc ggatcacatc gttcggcgga cccttcccga ctgctcggat
    14341 atcgagttcg tcccaatcga ccacggcgag atgacggcgg cggaactgcg gcgccatata
    14401 gtctccacgc cggatccgtt gcattgggat tgcttccgat tcggggtgat tcaggccacg
    14461 gactgcttca cggtttatgt gagtgtcgat catctcaaca tggacgcgac gttcgtcgct
    14521 gtggcgcaca tggagttcta tttgatgtat gcctccctgg taggaggcgg cgcccccgtc
    14581 gtgttgcccg aggcgggcag ctatgacgat ttctgcgtcc gcgagcacca gtacctttcc
    14641 acgctgacct cagagtcccc gctggtacgc gcctggaccg agttcgccga gaacaacaac
    14701 ggaagctttc cggatttccc gctgccactc ggtgatccgg cggtgccctg taccgccgga
    14761 atggtcaccg aaacgctgat gaaagagcac cagatgattc ggttcgaatc cgcctgcgta
    14821 gaggcggggg cccgtttcgt cggtggtgtg ttggcatgcg ccgcactggc cgagcacgag
    14881 ttgactggca gcgacaccta ttacggactc actccccgtg acacccggag cgcgccagcc
    14941 gatttcacga cagcggggtg gttcaccggc ctgattccga tcaccgtccc tatcgcaccg
    15001 gcgttcggcg atacggcccg tgccgcccag gcgtcctttg actccggtac cgaccttgcc
    15061 aatgttccgt tctatcgggt actggagttg gcgccccggc tgacctggcc tcgacccaac
    15121 taccccgtcg tgaactactt cgatgcaggc gtgcctccgc tctctgcttt cttcaccgcg
    15181 agactggacg caacgaacgt gggcgtctac accgacgacc ggtattccta tcagatgtcc
    15241 atcttcgtgg ttcgggtgga gaaagagacc agggtgatgg tgatgttccc gaacaatgag
    15301 atcgcccgcg catcggttgg ccgctatgta gatgcgatga aaaccgtctg tgcccgcgtc
    15361 gccgagggtc gcgccgcagt gcctccgcgc agcgccgctc agtccggaat tgcgttggga
    15421 accatcaagt gttagggctg agggacaccg ggcattgggg cgcgttgcgt aggcctggta
    15481 cgaatttccg cggtttgaca cgccagcaac gagtgtgtcg gtggcgggcg cacgcgatgc
    15541 gcatagataa accagaggtg gcggcgacgt gcgacggcta gccgatttca tggtgcggtg
    15601 gccctgggcg gtgatagggc tctgggtcgc gattgcgatc gcactgccgc tgacgttgcc
    15661 gagcctgaac gagatggccc agaggcatcc gctggccatc ctgcccagcg acgccccgtc
    15721 gagcgtcgcc gcccgacaga tgaccgaggc gtttcacgaa tcgggtacgg acaacctcct
    15781 gttggtggcg ttgatcaacg agaacgcgct caagcccgcc gacgaagccg cctaccgcaa
    15841 ggtggttgac gcgctgcgcg acgacgtgac agatgtcgtg atgttgcagg atttcgtcac
    15901 cacaccgcaa ttgcgcccgt tcctgacgag tgaggacaag acgacctggg tgctgccggt
    15961 cggccttgcg ggtgagttgg gcacgccgcg tgcctacgac gcctataacc gagttgccga
    16021 catcgtcaaa cagaatgtcg tcgccgacgg cccacttact gtgcacgtca ccggacccgc
    16081 ggccaccgtc gccgacctca ccgtcgcggg cgaacaggat cgcctgccga ttgagattgc
    16141 gatcggcgtc ctggtcctcg ccgttctgtt gctggtgtac cgcaacccgg tcaccatgct
    16201 gctgccactg gcggcgatcg ggatgtcgct tttgacggcc cagggcgtgg tggctggggt
    16261 ctcccaactc ttcggactgg gggtttcgaa ccagtccatc atatttctga ctgcgatgat
    16321 cgcgggcgcg ggaacggatt acgcggtctt tctcatcagc cgctatcacg attatctccg
    16381 actcggagcg gatctcgatg acgcggtgcg gcggacgttg atctcggtcg gcaaggtgat
    16441 cactgcatcg gccgcgacgg tgggaatcac ctttttcggg atcagcttcg ccaaaatggg
    16501 ggtcttttcc accatcggtg cgtcgtcggc tattgggatc ggggtggcgt acctcgccgc
    16561 gatgacgttg ctgcctgcca ttctggtcct ggccgggcca cgcggctggg tcaagccgcg
    16621 gcgcgaactc accgctcggt tctggcgacg gtcggggata cgcattgtgc ggcggccgaa
    16681 ggcccatctg gttggcagcc tgctcgtcct ggcccttctc gccagctgcg cgaccttggt
    16741 gcacttcaac tacgacgatc gcaaggccct cggaccctcg gcgtcgagtt cggtcgggta
    16801 tgccgcgctg gaacgccatt tcgacgtcag ccaggccatg cccgaataca tcctcgttaa
    16861 gtcaccgcgc gacctgcgca caccgcacgc cctcgcggac ctggagcaga tggcacagcg
    16921 ggtcagccaa ctgccgaaca tcggtcttgt cagcggcctc acccgaccca ccggagttgt
    16981 gccgcaggag ttcagagcca cctatcaagc cggcattgtc ggcgatcgga tggccgccgg
    17041 ctcggctgcg atcagcgaga acagcaagaa tctcaaccga ctgacgaacg gggccggcac
    17101 gctggccgcc agcctcggtg atgtgcgcgc tcaaatcaac cgaatcgctc ccagcatgca
    17161 gcgtctgatc gacgccttca gctcgatgcg aagccaatcg ggcggagaca agttggtcaa
    17221 gcaggtcgat accgccgccc agctcgtgca gagcatcaat gcgctcggca atgccatggg
    17281 cgtgaacttc aggaccgtca gggacatgtt cgcctgggtg ggtccggtgc tggcggcgct
    17341 ggacggcaac gcgatctgcg atgccaaccc ctcgtgcagc gatacccgca tgcagtttca
    17401 gaaactggtc gccgcgggtg acgacggacg cctcgaccag atcaataccc tggccgggca
    17461 actacagggg ttcggagacc gacagaccct caacgcaacg gtgaaacaac tcaatgatgc
    17521 gctctcgagc ttcagctcgg cggtgcgctc catgggtctg gaccaacccg gtggcctcga
    17581 ggcgggcatg acccgggtca ggcaggacgc caaccgcttg gcagatggga gccagcaggt
    17641 ggccggcgga gtcgaccaac tcgtcggcca agtcaaacag atgggtgccg gactcgatga
    17701 gtcggccgcg ttcctcttga cgatgaaaga tgacgcggga gatccgtcga tggcggggtt
    17761 caatcttccg gcccaaattc tgcaactcga cgagttcaag aaggccgcga agatattcat
    17821 ctcgccagat ggccactcgg tgcgctacct ggtgcaaacc aaactcgatc ccttcagcac
    17881 cgaagcgatg gatcaggtca atgcgatcgg tgacaccgct cggggagccc aaccgaatac
    17941 cacgctgtcg gacgcatcga tatcgatggg cgggtttccc gccgcacttc gggatacccg
    18001 cgactattac gagcacgaca tccgtttcat catcctcgta accttcgtcg tcgtgctact
    18061 gaccttgatg gtgctgctgc gtgcgatcgt cgcaccgctc tatctggttg tttccgtggt
    18121 gatttcgtat tttgcggcac tcggtatcag cgtcctggtg tttcaatacg cactcggcca
    18181 gcaattgcat tggagtgtgc ccccactggc cttcgtggta ttggtcgcgg tgggcgcgga
    18241 ctacaacctg ctcttcgtgt cgcgaatgcg cgacgagtcc ccgcacggta tgcgttacgg
    18301 cgtcattcgc accctgacct cgaccggcgg ggtgatcacc gcagcgggtt tgatcttcgc
    18361 cgcttcgatg agtggtctgc tgttttccag catcaccacc gtggtccagg gcgggttcgt
    18421 gatcggtgtc ggcgtcttgc tggacacctt cctggtgcgg accgtcacgg tgcccgccat
    18481 ggcgacgctg gtggggcgag cgaactggtg gccgtcacga ctgagctcgc agcggccagt
    18541 gaggcagcca cagtcacagt caccggccgg gcaggcggag tagggagtga agaaaatgaa
    18601 gcgagttctc gcattcgtca ctgcgctgac aaccttcggc gtcgcagggg gtttcggctt
    18661 cgggatcgcg gcagccgacg aagggccggt tacgccgacc gacccaactc cgaccgatgg
    18721 gccgccgccg ctggggacgc cggggcgggg gtacgcgctg ggaggcgcgc acgttctggg
    18781 cattccctat gacgagtaca tcaagcgcac gggcgccgac tggttcccgg gactggatcg
    18841 gcaaatcgtc gactaccccg caggccaggt tcagggccac accctggaac ggatgttccc
    18901 aggcatcggc cggctggatg acaaccttcc gggcctgggt gtcgatggcc ccagcatcgg
    18961 cgagtcggtc gacgagggtg agggcaacct cgaagcggcg atccgcaacg gtggtcccgg
    19021 caccacgatc ggcttgtccg agggagcgat ggtgctcaac gccgtacagg ctcgactcgc
    19081 caacgatccg accgcgccgc caccggatca gttgagcttc gcaacctacg gcgacccggt
    19141 agcccggcat gccttcggtg agagtttcct gacccagatg tttccggtcg gcagcgtcgt
    19201 accctcactc gactaccgca taccacctcc ggtggagagc cagtacgaca cctaccactt
    19261 cgtctccgcg tacgacagca tcgctgactg gccagaccgg ccggacaact ggattgcgct
    19321 cgccaacgcg gtcgtgggcc tcgcgaccgg ccacaccgcg gtggcgttca ccgacccgag
    19381 tatggtgccg ccgcaaaaca tcaggacgac cgtcaactcc aggggcgcga agacgacgac
    19441 gtacatgatt cccgagcagc accttccact ggtgctgccc ttcaaatact tcggggtgga
    19501 cgaaggcacg ctgaatcagc ttgacggggt gctgaaaccg atggtggatg cggcatattc
    19561 gcgaaacgac gatccgctga ccgctccgat tacggtggat ccggtgcgtg gctatgaccc
    19621 ggcgaccgtc accgcaccgg ccacgcaggc cgcctttggc ggcgccgcca ccgatcctgt
    19681 ttcgcagctc ctcgccggct tccagtatgt gttgaatcat cagcagccat gacccgagac
    19741 ggctgcaatg agcccgggcc agcaatcggt cccgagaggg ccttgtcggc gccgagcggc
    19801 tcccgaacag tcgtcctcgg tcgggttacc catgggcgca gttacggaat ccgactgtcg
    19861 cacatccggg cgccagttcg cgtctgaatc ttggggcgcc cggcacgggg cgtcgtggaa
    19921 cccgctgccc gtaggcggca gacgaagcgt tgcgccgctg gcccgatgaa accgtgctga
    19981 ccacggagaa ccctgaccga tgaccaagcc gcagtgggtg attggcgagt gacgactgga
    20041 gtgcagccgt gagcaggccc ggagcggatt ctcatacgac ctcgcgatct cgggtggtgg
    20101 cgtgggcgct gtgggactgt ggcgcgacgg gggtgaacgc cgtcgtcgtc acgtttgtct
    20161 tctccgtcta cctcaccgag caggttggtt cgggtttgcc cggccccgcc acgcccgcaa
    20221 gctggctggg ccgggtgctg acgatcgcgg gattgtgcgt cgcgttgctc gcgccggcga
    20281 ccggtatctg ggtgaacgcg cctaaccgcc gccgcccggc cctcgcacta ctcaccggcg
    20341 tggtcgttgc gatgacgggt gcgatgagcc tgatccgggc cgactaccac tatctctggc
    20401 ccggtctcgt cctgctggcc ttgaccgcgg catgcagcga tttggcaacg gtgccgtata
    20461 acgccatgtt gaggcagctg tcgacggggg agacctcggg tcggatttcc gggcttggtt
    20521 cggccgcggg atacgtcggc agtgtgctgt tgctgctgat tatctatttc ggcttcatcc
    20581 agggcgacgg cgacacccgc ggcctgctgg gccttgcggc ggtcgacggc gggaatgtgc
    20641 gggcggcgat gctgctcgcc gcggcgtggt tggcgctgtt cgcctggccg ctgctcgtct
    20701 gcgtgcgcac cccggccttc gtcgaccccc cgccgactgt cgggttcctc ggcgcctatc
    20761 gcgcgctgtg ggacgaggtg aagggggagt ggcgacgcga ccgcaacgtt gtctactacc
    20821 tcttggccag cgcggtattc agggacgggc tcaccggtgt gttcgccttc ggcgcggtgc
    20881 tcggcgtcag cgtgtacggg gtgtcgcaag ccgacgtgct ggtgttcggc gtgtgtgcca
    20941 gcatcgtcgc cgcggtcggc gcggtgctgg gcggcctgct cgacgaccgg gtgggctcca
    21001 agcccgtcat cgtcggctcg ctcgccgcga tgatcgccgt cgggctgctc ctactcaccc
    21061 tctcggggcc gctggcgttc tggatctgcg gactgcttct gtgcatgttc gtcggcccca
    21121 tcctggcgtc tgcgcgggcg ctgatgctgc ggatgtcggc ggagggcaag gaaggcgtgg
    21181 ccttcgggct gtacacgacc accgggcgcg cagtatcgtt cctggcgccg tggatgttct
    21241 tcaccttcat cgacctgttc gggaccggcc gggccggttt gggtggccta tgcgtggtgc
    21301 tggtggcggg cctactggcg atgatgatcg ccgtgcgggt gccccgcgag gcgaactagt
    21361 acttgccgca tgtactcggt gaagacgggt ctgtgccggc agcatcacga tcgcccctcg
    21421 cgttcgcctg ccgtcgcagt actccggcga cggtgtctgc ctcagccgcc acggatggcg
    21481 ctcagatcgg gcttgccgga cggtgtccgg gggatcgcgt cgacgatcct gatttcggtg
    21541 gggatctcgt agcgcgccag ccggttccgc aggaactcga gcagctcgct cgcgtcggcg
    21601 gaggcggcgt cgcgcagctc gaccaccgcg acgggtgttt cgccgagccg gccgtcagga
    21661 cgacccacca ccgccgcgcc ctgcacggcg ggatgacttt ccagggcggc gcggacatcg
    21721 tcgggcatca ccttgaagcc gccgcggatg atcgcctggt cggccctacc gagaatccac
    21781 aggaagccgt cctcgtcgat gcgtgccatg tccgtggtgc gcatccagtc cgccgacggc
    21841 ccgagctgac ccggtttgac ctggagcagg cccggttgat cggcgcccag cggcttgccg
    21901 tcgtcatcga cgacacgcag ttcggcgcct gggtttgcgc gtccgacact gccgcgcttg
    21961 gcctgccagt acttctgatg atcggccagc gtccagccgg ccacaccacc gccgaattcc
    22021 gtcgcggcat aggacgtcag cacagggatg ccgaacttct gctggaaggc gtcggcgtcc
    22081 tccgcggaaa gcggcgcggt acctgatgtg acggcacgaa tgctttcgag gtcttcgggg
    22141 cgcacggcgg agtgcaacac catccgcagt gcggcgggca ccagcgagac cgcgcggggg
    22201 cggtgcctgc gcacggcgtc gacccatgtg ccgagttcga accggtccag cagcgcgaac
    22261 ggtcgggcct cggcgacgca ttgcagcacc cggaacaccc cgccgatgtg cacgaggggc
    22321 gcgttcacga tcgcgacgcc gccgcgaact tcgctccgtg acggcgaccg gcccgggtcg
    22381 cgacccatca cgctgtgcgc cagcatgtcg taggtgaggt cgatgcgctt cggtggtcct
    22441 gtcgtcccgc tggtcaacat ccgcaccgcg acaccggatc tcgtcgcccg gtggccgtcg
    22501 ccgaccgcca ccagctgcgg ctcggcgtcg agcaccgaaa gagaaatcac cgtcgacccg
    22561 gcgctcggtc cgaccagttt cgtcaggtcg tcggggtcgc cgatgatcag cggcaggtcg
    22621 agcgctgcga tgtccgcctt ggtgcggtcg tcgccacggg acgggttgat gaccaccacc
    22681 gacccgccgg ccagcagcac gccgaggaac gcggcgacat gcgtcggccg gttgcgcagc
    22741 agcatcccga cctgaccacg gccggcggcc atcgtggcaa cccgccgggc cagcacgccg
    22801 atctgcccgt aggtgaacca ctggccgtcg tactcgatcg ccgacgcgtc gggcgccaga
    22861 tcgagcacgg cggcgatgcg ccggcacagc ggatgtccgg ccatcaccgg atccgtggct
    22921 cggatttcac cggcccgccg cgctgcgcga gttccgccgc gccgatcggg ttgcccagcc
    22981 gggtgtaaat caggttctgt tcgagcgcgg cccgatacgg cttgtccagc gactcccaga
    23041 tcgccttcac ggtgccctgc gtcgccgacg gtggcttggc ggcgatcgtc gccgcgatgg
    23101 cgtgcgcacg ctcccatagc cggtcacgca gcaccacctc ggataccagc ccgatccgca
    23161 gcgcggtgtc ggcgctgacg cgttcgtcat tgcccatcaa cgcgattcgc agggtctcac
    23221 caaggcccac gcgccgcatc aggccgatcg gttcgagcgc acacaccagc ccggcggaca
    23281 cgtgtgagtc gaagaacgtg gcgtcctgcg aacagatcac gacgtcggat tcgttgacga
    23341 agtagaacgc gcc
//
LOCUS       DASYLX010000992         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2039537,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000992 DASYLX010000000
VERSION     DASYLX010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039537"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGP27_23150"
     CDS             complement(<1..725)
                     /locus_tag="VGP27_23150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7854230.1"
                     /translation="MSNPFRFGLIGGGRMGRTHLRGLEGSTDVEVVAVTEPAETAAAA
                     LRERGLSVFSTADDMFAGSELDGVLIAVPTPQHIDTTRRALEAGLPVLCEKPFGLVPD
                     DARAAGVLARDKGLALQIAYWRRFVPRLSTLRGDLADGRYGTVHFLVCAQWDEAPPPL
                     SFRNDSGGIYIDMGVHEIDQTLWLLGQDFDEVTAQAYPTTEDAGAQNDVDSTQALVML
                     SGGASAVISLGRFYKGGDVVSVAL"
     gene            complement(722..>1688)
                     /locus_tag="VGP27_23155"
     CDS             complement(722..>1688)
                     /locus_tag="VGP27_23155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7854231.1"
                     /translation="RQLERMRNMSNDSEIIVANAPVSYGAFEVTIGFDPNVPDGDDIL
                     DQVSAAGYAGIDLGPVGYLGSTENLGAKLAKRGLGLAGAYLELPYADPEMLEKTLPEL
                     DAMLDIFDAIKPLGLGPDPRPTLADLGVGDGADFRRNNPGSGARNPASGYTEEQWAEF
                     ARGMAIVVDRCRSRGYQPVFHNETGTFVEAPEEVSRMLAVSEIGLCMDAGHFLVGGGD
                     PVEYVKKWSNRIDHVHIKTADLPRFQQIVDKGLPTNAIWEHEVFPKLGEGALDVDAFL
                     AALREIDYAGWLVVEQDIFPTTAERFAQAAADQRANRQFLLERGL"
BASE COUNT          289 a          582 c          521 g          296 t
ORIGIN      
        1 agcgcaacgg agacgacatc gccgcccttg tagaaccgcc ccagcgatat caccgccgat
       61 gcgccgccgg acagcatcac cagcgcctgc gtgctgtcca cgtcgttctg agcaccagca
      121 tcttctgtcg tcgggtacgc ctgcgcggtg acctcatcga agtcctgacc gagcaaccag
      181 agcgtctggt cgatctcgtg cacacccata tcgatgtaga ttccgccgct gtcgttgcgg
      241 aaagacagcg gcggcggggc ctcgtcccac tgtgcacaca ccagaaagtg cacggtgccg
      301 tatctgccgt cggccagatc gccgcgcagc gtggacaacc gagggacgaa tcgacgccag
      361 taggcgatct gcaaggcgag gcccttatcg cgcgcgagaa cgcccgccgc cctcgcgtcg
      421 tccggcacca gaccgaatgg cttctcgcac aacaccggca ggcccgcttc cagcgccctg
      481 cgagtggtgt cgatgtgttg cggtgtcggc acggcgatca acacgccgtc gagctccgac
      541 ccggcgaaca tgtcgtctgc ggtggaaaac accgacagcc cgcgctcgcg cagcgcggct
      601 gccgcggtct ctgccggctc tgtgacggcc accacctcga cgtcggtcga cccttccagg
      661 ccccgcaggt gggtgcggcc catccggccg ccaccgatca ggccgaaccg aaaaggattg
      721 ctcacaggcc ccgctccagg aggaattgac ggttggcccg ctgatcggcg gccgcctgcg
      781 cgaaccgctc cgcagtcgtc gggaagatgt cctgctcgac gacgagccaa ccggcgtaat
      841 cgatctcacg cagagcggcc aagaacgcgt cgacgtccag cgcgccctcg cccagcttcg
      901 ggaagacctc atgctcccag atcgcgttgg tgggaagtcc cttatcgacg atctgctgga
      961 aacgtggcag gtccgcggtc ttgatatgga catggtcgat gcggttggac catttcttca
     1021 cgtactcaac cggatcgccg ccgccgacca ggaaatggcc cgcgtccatg cacaggccga
     1081 tctccgaaac cgccagcatt cggctcacct cctcgggcgc ttcgacgaat gtgcccgtct
     1141 cgttgtggaa caccggttgg tacccgcgcg accggcaccg gtcgaccacg atggccattc
     1201 ccctggcaaa ctcggcccac tgttcctcgg tgtatccgct ggccggattg cgtgctcccg
     1261 aacccgggtt gttgcgacgg aaatccgcgc cgtcgcccac tccgaggtcc gccaacgtcg
     1321 gacgtgggtc cgggcccagt ccgagcggct tgatggcgtc gaagatgtcg agcatcgcgt
     1381 cgagttcggg gagggtcttc tccagcatct ccgggtcggc gtagggcagc tcgagatatg
     1441 cacccgctag acccaagccc cgcttggcga gcttcgcgcc gaggttctcg gtgctgccca
     1501 gatagccgac cgggccgaga tcgatgcctg catagccggc agcgctgacc tggtccagga
     1561 tgtcgtctcc gtcaggaacg ttcggatcga atccgatcgt cacctcgaag gcgccgtagc
     1621 tgacaggcgc gttcgcgacg atgatttcgc tgtcgttgct catgtttcgc attctctcta
     1681 gttggcgg
//
LOCUS       DASYLX010000993         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3574286,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000993 DASYLX010000000
VERSION     DASYLX010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574286"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1162
                     /gene="carA"
                     /locus_tag="VGP27_23160"
     CDS             35..1162
                     /gene="carA"
                     /locus_tag="VGP27_23160"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV7854232.1"
                     /translation="MVLDDGRIFTGTSFGALGETLGEAVFSTGMSGYQETLTDPSYHR
                     QIVIATAPQIGNTGWNHEDAESRGDKIWVAGYAVRDPSPRASNWRATGTLDDELVRQG
                     IVGIAGIDTRAVVRHLRSFGSMKAGVFSGPALAATSGSGDAAMDELLNRVRSQPSMLG
                     ADLAGQVSTDGGYVVETDGPQRFTVAAIDLGIKTNTPRNFAQRGIRSHVLPSSTTFEQ
                     IADLKPDGVFLSNGPGDPATADHVVDVTREVLGAGIPLFGICFGNQILGRALGRSTYK
                     MVFGHRGINVPVIDHITGRVAVTAQNHGFALEGEAGEKFDTPFGAAEVSHTCANDGVV
                     EGIRLVDGRAFSVQYHPEAAAGPHDANYLFDQFIDLMSEER"
     gene            1440..>1751
                     /locus_tag="VGP27_23165"
     CDS             1440..>1751
                     /locus_tag="VGP27_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854233.1"
                     /translation="MPRRTDLNHILVIGSGPIIIGQACEFDYSGTQACRVLRAEGLQV
                     SLVNSNPATIMTDPEYADHTYIEPITPAFVERVIAQQAERGNKIDALLATLGGQTALN
                     TA"
BASE COUNT          318 a          607 c          553 g          273 t
ORIGIN      
        1 gacgagcgct ggtcgcgagc agcgcagggc acagttggtc ctcgacgacg gcaggatctt
       61 caccggcaca tcgtttgggg cgctgggcga gacgctcggc gaggcggtgt tctccaccgg
      121 catgtcgggc taccaggaga cgctgaccga ccccagctat caccgccaga tcgtcatcgc
      181 caccgcgccg cagatcggca acaccggctg gaatcacgag gacgcggaga gccgcggcga
      241 caagatctgg gtcgccggct acgcagtacg agacccgtcg ccccgcgctt ccaactggcg
      301 ggccaccgga accctcgacg acgaactcgt caggcagggc atcgttggca tcgctggcat
      361 cgatacccgc gcggtggtcc ggcacctgcg cagcttcggt tcgatgaagg ccggggtgtt
      421 ctccgggccc gcgttggcgg cgacatcagg ctcgggtgac gcggccatgg acgagctgct
      481 aaatcgggtc cgcagccagc cgtcgatgct cggcgccgac ctggcgggcc aagtcagcac
      541 cgacggcggt tacgtcgtcg aaaccgacgg gccgcaacgg ttcaccgtcg ccgcgatcga
      601 tctcggcatc aaaaccaaca cgccgcgaaa cttcgcccag cgcggcatcc gcagccatgt
      661 gctgccgtcc tcgacgacct tcgagcagat cgccgatctc aagcccgacg gtgtgttcct
      721 gtccaacggg ccgggagacc ccgcgaccgc cgaccacgtc gtcgacgtca cccgcgaggt
      781 gctgggcgcc ggaatcccgt tgttcggcat ctgtttcggc aaccagattc tcgggcgcgc
      841 actcggccgc tcgacctaca agatggtgtt cggtcatcgc ggcatcaacg ttccggtcat
      901 cgaccacatc accggccggg tcgccgtcac cgcgcagaac cacgggttcg cgctcgaggg
      961 cgaagcgggg gagaagttcg acacgccgtt cggcgccgca gaggtgagcc atacctgtgc
     1021 caacgacggg gtcgtcgagg gcatcaggct cgtcgacgga cgcgcgttct cggtgcagta
     1081 tcaccccgag gcggcggcag gaccgcacga cgccaactac ctcttcgacc agttcatcga
     1141 cctgatgtcg gaggaacgct agcgatgcca gtcatctcac ccctcaccgc gacggtgcgt
     1201 gtctgcgcca cgacacgccg caatattcgt gcaaacgcgc acgctcgtcg tacgtcgaca
     1261 ctcaacacga ctgtgccaat tcataccgtc agccggcgtg tcgcgtcagt aaccgcacag
     1321 tcgccgaacc acgggttttt tgcgcccgct cacttgggcg cgagcgtgcg caaacgctcg
     1381 aaattcgtcg gcgtgtcgtg tgcagacacg cacgctcgcg gtgcaaggcg ggccgtgtaa
     1441 tgccgaggcg gacggatctc aaccacatcc tggtgatcgg ctccgggccg atcatcatcg
     1501 gacaggcctg cgagttcgac tattccggca cgcaggcctg ccgcgtgctt cgcgcggagg
     1561 gtctgcaggt cagcctggtc aactccaacc cggcgacgat catgaccgat cccgaatacg
     1621 ccgaccacac ctacatcgag ccgatcaccc cggccttcgt cgagcgggtc atcgcacagc
     1681 aggccgagcg cggcaacaag atcgatgcac tgctggcgac tctcggcggc cagaccgcat
     1741 tgaacaccgc g
//
LOCUS       DASYLX010000994        22729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1379774,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000994 DASYLX010000000
VERSION     DASYLX010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22729
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379774"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="dcd"
                     /locus_tag="VGP27_23170"
     CDS             complement(<1..453)
                     /gene="dcd"
                     /locus_tag="VGP27_23170"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV7854234.1"
                     /translation="MLLSDRDLRSEIAAGRLGIDPFDESLVQPSSVDVRLDNLFRVFN
                     NTRYTHIDPSVRQDDLTTLVEPKEGEPFVLHPGEFVLGSTLERCTLPDDLAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCL"
     gene            512..585
                     /locus_tag="VGP27_23175"
     tRNA            512..585
                     /locus_tag="VGP27_23175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:544..546,aa:Gly,seq:ccc)
     gene            755..2086
                     /locus_tag="VGP27_23180"
     CDS             755..2086
                     /locus_tag="VGP27_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7854235.1"
                     /translation="MHYARNGDVRLAYRVFGTSGPVVVWIPGWVVGNVDNIDEPGSPY
                     APILDLASRDIRLVVWDRRGTGLSDPSTHLLSLDETVTDLRAVLDAVGDDQPSLFGTG
                     EGGPVSILYTATYPDRIGSLALYAAAARFSQELPDFPWGFTPDEIDAQLEDIDNHWGE
                     GALIELFHGAASDSPGVRELFGKLQRSVMSPSSAKLWWRATMEVDVRAVLGTVRVPTL
                     VLARQGDGLVPIESAIALATGIPNAQFHELSAGAHTPFDILHELAEQTRRFFIDKPDV
                     PVEERLLKTVMFTDIVGSTEQLSASGDANWRHQLDTHDKVVDWLLEKYGGHRAKHTGD
                     GVFALFDGPTKAARCALDLVPALATRGIRIRVGIHTGECERRGDEWSGLAVHTGARIG
                     ALASTGEVLASRTVRDLSAGSGLTFEGLGQQRLKGLPEEVDVYRVTAGSAR"
     gene            complement(2064..2291)
                     /locus_tag="VGP27_23185"
     CDS             complement(2064..2291)
                     /locus_tag="VGP27_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854236.1"
                     /translation="MRHTYASLCLAAGIRPIDIAELMDHRDVKTTLTVYAHLINTDDH
                     TGNMAALGALATPTTKADNVILLRANAQNLQ"
     gene            2773..3621
                     /locus_tag="VGP27_23190"
     CDS             2773..3621
                     /locus_tag="VGP27_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854237.1"
                     /translation="MTDEVRPDPVPRERRRRGAAGLLDSFLDSPISGLAPWIVLSVFA
                     GPGHFKEAISAVFGFSLLMLWASRRRGDRMHSLSVFGVVYFGALLLVRVLAGPGTESW
                     LSSWTGTMSNIALAVFATGTLLIRRPFTLSYAKETTPEEFWGLPGFYRTNLVISAVWA
                     ASFAVGGVLGVIGNVVLHDSDNFWTGWILQLGATFFAMAVTGVYPDYAGVKMEREAGI
                     TMEPLPSLLPFIDWLPMYVLITGIIGWVIDEVPDWLGITLIVVGAIGVAALRKFAPSA
                     EREPSG"
     gene            3689..3761
                     /locus_tag="VGP27_23195"
     tRNA            3689..3761
                     /locus_tag="VGP27_23195"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3721..3723,aa:Ala,seq:tgc)
     gene            4132..5403
                     /locus_tag="VGP27_23200"
     CDS             4132..5403
                     /locus_tag="VGP27_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV7854238.1"
                     /translation="MQDHLPAPAISRSVRVLGPVVLLVALALYLAAYLRWPSLAGQVD
                     LLVYRFGAVRAWDGQDLYSVGMTGDSHTMLFDYTPFAALCFLPLIGLSQPALLTLGLL
                     VNIACVGYIVRRMLTAAGITTVTGLWGLTALLMAPIIWLEPVRLSLQLGQINLIMMAI
                     VLADVLSQPSRRWSGIGIGIAAGIKLTPALFIIFLVATGRRREAVIAGSTFISTVAVG
                     FVLLPADSNEFWLRGRFHDVRRISSDPLANTSLAGLLARLNWATRWETAAAVVFAIVA
                     IAIAAVAWRRGHLLLGVAIAGMTSAAASPFSWSHHWVWFAPLLVHLGLLGSVHRSRVA
                     ALAMWTLAAVFGGWFIASRSSPPQAGLMSLRHPGTWNQLLPAAYLFALTIVLVCSMYT
                     LWRARQHPAAATIGIDAMVEGSRPRASIGPL"
     gene            complement(5744..6541)
                     /locus_tag="VGP27_23205"
     CDS             complement(5744..6541)
                     /locus_tag="VGP27_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854239.1"
                     /translation="MLFLYHLPNWLMAVCIVGAVVALVFPGYVLIHRLWRRSFTEAET
                     NVSMAVLTVIATIYALLLAFVAVSVWQSFGAAETAVVNESNSVGQLARALSLFDSPQA
                     REARGTLREYADVVVTVEWPDMRRGQSSTEAWDAFDRMFHAVGQLEPDTPRRAALMPE
                     IWSAANDVLSERRTRLHAADAKVPFTLWMVVLIGSALTIGTTAVLSPSRFSFWIIGLL
                     AMSMGLVFFLIVVMDRPFTGEESIGPAPFHTAIENMDRWDAEFAHSK"
     gene            6851..7591
                     /locus_tag="VGP27_23210"
     CDS             6851..7591
                     /locus_tag="VGP27_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854240.1"
                     /translation="MLLLSAAPLLAISAHVFGLAPQNTTALLVIFPLIVGLAVLIAFS
                     PHRIDMVLGRGLLAGMVACLFYDAFRLFCVHVLALMDDFIPVMGTWITGGSHGAASAV
                     VGYMWRYLGDGGGLGVAFFAIALAVGLGRRSTRPVRVVLAAVGFAVFVWAGLVATVVL
                     APRGEELMFRLTVSTLAVTLIGHVIFGLVLGLGFLQARRGWYGPELEWPLILSVPTRL
                     RAHNVAQAHSRAHSRVVRTVEADTPKPL"
     gene            complement(7625..8416)
                     /locus_tag="VGP27_23215"
     CDS             complement(7625..8416)
                     /locus_tag="VGP27_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854241.1"
                     /translation="MSRWLVNDVPSWLLLTGLIVLIVGGSVLVQVYVRKRFPRLKDGA
                     HNDVTKFVYGVVGFVYAFFIGFVVSAMWGQVNVADEETRSEGAAGVQLARDIYVFDKA
                     DGDRIRRSLLDYEKAAIAEWPLLANGQRVSDADEALHRLYVAYTDVKPQTDTQKTFLA
                     TSFSNLNDLSKARTERVLLASTDTGPPWSLWVVIFLTSGLVLGCSIIYGVGNAAMHYP
                     MVATVSALVAANTFLIVQLSHPLVGDIATSPAPLSEVVQVLSAPR"
     gene            8447..8647
                     /locus_tag="VGP27_23220"
     CDS             8447..8647
                     /locus_tag="VGP27_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854242.1"
                     /translation="MRAASTMVGTDDTVTPGPLSRHVELFSGGESPTSFSFDRTMIIA
                     DNRNGVGNEKHYARVSPRGDLL"
     gene            8952..11054
                     /gene="glgX"
                     /locus_tag="VGP27_23225"
     CDS             8952..11054
                     /gene="glgX"
                     /locus_tag="VGP27_23225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV7854243.1"
                     /translation="MTPPRRDDAPEKGVAQPLGATVTPTGVNFSVYSEHATGVTLLLF
                     DSHEARTPAREVTLTARNNKSFHFWHCHLSGVGPGQVYAYRMDGPFEPGSAGLRFNRN
                     KVLIDPNAFGHIDNLWDRETAVGPEDNVATSMRSVVIDPTGYDWEGDRPLNIPLTETI
                     IYEVHVRGFTKSPTSKVEHPGSFRGVVEKIPYLKSLGITAVELLPIFDFDESQVLRSG
                     PTGQSLTNYWGYDPYCHWAPQSTFCISPHLGTHLDEFRDMVKALHRAGIEVILDVVFN
                     HTGEGNQNGPTISFRGQANEAYYMLSKDDRQYYMDYTGVGNTFNANHPVVTKYIVECL
                     EYWVREHHVDGFRFDLGSALSRGPDGAEMAVPPVLWNIELSRVLWDVKLIAEAWDAGG
                     LYQVGRFPGNRWSQWNGPFRDCVRKSVKSDPGLVATLATRIAGSSDLFGPSGERPTNS
                     INFVTCHDGFTLNDLVSYDAKHNEANGEGNRDGADDNNSWNSGFEGPTADGDLERLRE
                     RQIRNFAAILLLSRGVPMFVAGDEFRRTQLGNNNAYCQDNELSWIDWTLVERHADLVR
                     FWTKMIGFRKRFPTFRQPDFFTGEVNVRGLADVSWHGSDLGEPDWHDPGSAVLSFTLA
                     GLEGADDVHVILNMSSQGIDFALPGIGGRRWARALDTTLASPDDIVDPGEEVVVESDK
                     YHAGERSVVALISQEVQS"
     gene            11051..13195
                     /locus_tag="VGP27_23230"
     CDS             11051..13195
                     /locus_tag="VGP27_23230"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEV7854244.1"
                     /translation="MKDMPFTVSDTIAGYVVSYDDDENSFVLRTTDGREYPVHLTPGT
                     FASVIRNFGEDYHDAGSQFEELLYPGRYLYAYGIYYPEGRERRFEVKSIIFPGVAGQE
                     YMFEKPDWWVNQARAVADFYLRGQFPDHNYDWRNYRTKLTLSGTHTPDYVAPDFRQET
                     DTISRMVYGLATAFLLTGEDRFLEAAEGGTEYLRKNMREVRADEGVVCWFHAIDVSPK
                     GQRKISASEFGDDFEAIAMYEQIYALAGPTQTYRVTGDPLILDDIEKTIALFDRYFRD
                     PDQGGYFSHLEPVTLDPRSELLRHNRARKNWNSVGDHAPAYLVNLLLALGRKEHEDFL
                     ATTGETIAARFPNEDSPFVQERFHEDWTSDRHYKWQQDNAVVGHNLKIAWNLMRINNI
                     RPNAAYVELAKKIAETMPAVGSDQQRGGWYDVVERTLKPGQAWHRFVWHDRKAWWQQE
                     QAILAYLVLAGTFGDGEYLRHGRESASFYNAMFLDHDDGGIYFNVLANGLPYLVGDER
                     LKGSHSMSAYHSIELCYLAAVYTNLLIRDQAMELHFKPKASSFPDGILRVAPDLLPAG
                     RVMLSNVWVDDEPYAEFDKVNLTVKLPKDKAVRVRVRLVSTNAEFDSHVQQREGEYHL
                     TLIGKIPRKRVNQLIAELERALLADATRLVLIVNELESLSEDALSALFLFRSKVKLEQ
                     EVVIVGASDAVMSTFTNCDSSTGGEFVYQETL"
     gene            complement(13211..13786)
                     /locus_tag="VGP27_23235"
     CDS             complement(13211..13786)
                     /locus_tag="VGP27_23235"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7854245.1"
                     /translation="MRLQGRKIAILVESDFYEPEIYYYQHRFAEEGAEAHFLSRLWGQ
                     PSIEFKGHELRAPLRCDESFEGIGDENLGQFAAVIVPSGMVSDRLRYTDDVEKLPPAT
                     EFMRRVFARPDIVKGIICHGMWLLAPTPELVRGRRVVVHNNLFGDVKNMGGIYVDADV
                     VVDGDLVTGRAGTVFHLFARKIIDMLAGDTA"
     gene            complement(13857..14660)
                     /locus_tag="VGP27_23240"
     CDS             complement(13857..14660)
                     /locus_tag="VGP27_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854246.1"
                     /translation="MVRWIVTNIPSTLILVILVACICAATIAGLTFVRRRFPGLAEGK
                     HNDVTNVGMSVVGPVYGFFIGFIVAFLLSQLATEDEWARTEGTAAISMTRELNVFGKA
                     DGDRIRQSLMEYEQAALAEWPQAANGRPTAEAETALSGLYSAVQNVEPGDDRQRVVLM
                     SALRALKELSDARTERLLSADAGFSPPLALWAVIFLSSGLVLAFAVAFGAKQQRWHYA
                     VVTAVSVLVAANLFLVIELAYPYLGVFAPTPEPLHAVIKMVQGQLPQPS"
     gene            complement(14852..16393)
                     /locus_tag="VGP27_23245"
     CDS             complement(14852..16393)
                     /locus_tag="VGP27_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854247.1"
                     /translation="MVAAGTVVPAPTAADTSAPDTYVDQLAEAADTPTLDWVECADGF
                     DCATAQVPLDYHRPGDAQITLAVIKLAAPDPSKRIGTLFINFGGPGQSGVDRLRQRAR
                     WPWLFSDALRSRFDLVSWDQRGVSRSAGVRCFSNAAEQSQFLRPSPGLPADADGEQKL
                     FAWSGELADRCRQQAGPILDHVSSANTARDLELLRRAVDDPALSYHGMSYGSVLGATY
                     ANLFPGRVRAMVLDGSIDFDGNANGHGSEGTTVPLNTRQDVAGGTEAAFDQFLRDCRA
                     AGPKCAFSHGDPVAKWAALAEHTRGTTILVEDHMWTVPEMIVATLAKPSTYPQLAQLL
                     QEISDTGAAAPELLDAVTGSGPPETDVEDPYLSNREEAYDAIQCVDSTVPTDPSSYSL
                     AAVTADESSPDFGRISVFDVMPCAFWQGYDPDRYTGPWNRRTSAPILVLNTRNDPATP
                     LPGAYAGASQLSEARVVVTEGAGHTSMYVASTCTERVKRDYLFTGVLPPSGTGCRRDH
                     SPFQG"
     gene            complement(16461..17729)
                     /locus_tag="VGP27_23250"
     CDS             complement(16461..17729)
                     /locus_tag="VGP27_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV7854248.1"
                     /translation="MVIVFAQETRDVVNALTWARQNDVALRVRSGRHCLEGWNNVDNG
                     IVIDVSEMKGAKIDTVAGTAIVGAGLNQMEAVTALGKEGLAAPTGTEGTVGLVGATLG
                     GGFGLLSRPFGMASDNLMAAEIVVASGADGAKAISVDEKNDPDLMWALRGAGNGNFGI
                     VTSLTYKVHPLPQVAYVTATWPGLNDVQAVFDAWQTSAPNTDDRLTSQMEVHPDSVLL
                     FGVLASGTEAEARQLLAPLLSIGKPEVAVKIGPWAEIYSGFQIPTPREPANLKFFSQY
                     ITESFPAEAIDIVRSFMAKAPSPDCNYFIQPFGGRVKDSEPSGGSAYAHRAANFYGEI
                     GAGWGTRGGGVPAHSDPVTITAQAWIAEFSQALRPYVNGAYVNVPNVGMSDWEKAYWG
                     PNVKRLRGIKARYDPTNVFNFEQSIPPASD"
     gene            18319..19134
                     /locus_tag="VGP27_23255"
     CDS             18319..19134
                     /locus_tag="VGP27_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854249.1"
                     /translation="MRTTMTGIGDPPLIFVHGFACDGTDWQAQRESLEKKTTVIVCEL
                     PGHGFSPGTSAECTIEAYGAAVAKTLRELPSRPAILVGHSMGCRVVLEANRVQSDAVA
                     ALVLVDGSRISSGDPIAAEQAMADELAGDGYRRFVRQFFESMFFPSSNPALAMAIVER
                     ALRFPTGLGRTLMTDLAGWDAREMESALDSVHVPLLAIQSTTLDTARQRVSLEPGLSS
                     PWLELVSAHVPQASIEMLHGYSHFPQIELADEVTALIADCAWPASHRYVRGRA"
     gene            19380..19910
                     /locus_tag="VGP27_23260"
     CDS             19380..19910
                     /locus_tag="VGP27_23260"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEV7854250.1"
                     /translation="MPITSFTAFSDGLEPQQRSRAHFVADTDLDDDRLWVPWGETEGV
                     WFQPCCFNVTAGGFSIVVKALPGAVLGAHYHVGDVYGFTMRGHWGYLEHDFLATPGTF
                     IYEPAGEAHTLFVAKDSPGPMMTFFVVGGGLLFLDKPVDGTFESYEDGFTFLEMARKH
                     YHDMGLDAAQLDERIR"
     gene            19913..20914
                     /locus_tag="VGP27_23265"
     CDS             19913..20914
                     /locus_tag="VGP27_23265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854251.1"
                     /translation="MTVTSNGFARHMLCGLRILVAIFGLTSMASCTSSSSAPDPNAGG
                     SEIIGNQLPSALLGVWTREWIRRPGVAVTDQNAVQNSPLVVRYLQTPEFFGDMRFPVD
                     RPDLSRASSFNDLTDEELRALSKQQGFFGYTTVVKDSNSGEPSRPPSGPTVQWHRQMD
                     FEPPTSNLDIGRLDFLSADLMNESAPDDSYTERWVSITNGENRFFVVYTEHIGRLDRM
                     LLVAGDQFFYGRNRAKDLPPAPSLDSLLSGASRGQMIEYLDLELSVGRVRGGLFPWEI
                     QYSTLPWREGKPAEFVRSIHVDDSTNKVTLSVGRDDTWKMPTNTLKPTDLRLLFPEK"
     gene            complement(20966..22291)
                     /locus_tag="VGP27_23270"
     CDS             complement(20966..22291)
                     /locus_tag="VGP27_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854252.1"
                     /translation="MGARDGGYLAKLCAVPQLEVKNENQAAVCITPQGDAVSIVGDRT
                     FTTTHKADAYRQEVHKCAGEALPPVWFRFYDGPNMFAKANYDEISFSMPVTKSALPPI
                     HNSQSLWDQMTILQPIPVFDDVKPTYCGFLGGKTLWANGGFNFELTAGSTGFDPGDAP
                     PQTPWNSPWQSGFNSTTYDGWTAYPTMALGNGAGGPPPGFAIRTLGSPDDTLTAPDSS
                     VHAKYSLDNDKQVVTLELSYNGATSTYSVPQQLFKDKFLPKAIADGSLSADDKQVGQV
                     NNFDWRWQLFTNTLTIQKPTAPDGKPITLNELAQYFPKTTSYNNIQAKVKGYSGFNNP
                     DAFENTLVCAKGFEMYGSIIDGKLFTDLDEYSKWVETEADKTDPRYSYWRCIEAIARS
                     REQNCVASGSEKDAAQQSWNVGGGYMNGTIPTQCDENDPETKAVIAGRR"
     gene            complement(22586..>22729)
                     /locus_tag="VGP27_23275"
     CDS             complement(22586..>22729)
                     /locus_tag="VGP27_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7854253.1"
                     /translation="NVRIPGAPKYREYMRQQQGKQFETIEHNTHGEYQTVVRDLVLES
                     VLL"
BASE COUNT         4221 a         7032 c         7040 g         4436 t
ORIGIN      
        1 taggcagagt tggccgatct tcatgcccgg ccacagggtg atcggcaggt tggcgacgtt
       61 agacagctcg agggtgatgt gcccgctgaa gcccggatcg atgaagcccg cggtcgagtg
      121 tgtcagcagg ccgagccggc caagcgatga cttgccctcg aggcggccgg cgaggtcgtc
      181 gggaagggtg caccgttcca gggtcgagcc gagcacgaac tcgccgggat gcagcacgaa
      241 gggctcgccc tccttcggct ccaccagggt ggtgaggtcg tcctggcgca ccgacgggtc
      301 gatatgggtg tagcgggtgt tgttgaatac ccgaaacagg ttgtccagcc ggacatcgac
      361 gctcgacggc tgcaccaggc tctcgtcgaa tggatcgatg ccaagacgtc ccgcggctat
      421 ctcgctgcgc aggtcgcgat cggagagcag cacccgacga gcgtagccgc tggagtgtta
      481 gccttcgtga tcaccggcac cagccggaca ggccgatgta gttcaatggc agaacatcag
      541 cttcccaagc tgaatacgcg ggttcgattc ccgtcatcgg ctccactccg agcaggacaa
      601 acccgcacgg tcgcccgtcc gcttggggca gtggtcccgt aacggtcccg tacccagact
      661 cgacaacgct gcggcggtgt gggagcagca cgagttcaaa tctgaggcgt tcggacctct
      721 gtgatgccac gatgttcacg tgtctgtcgg acgtgtgcac tacgcgcgca acggcgacgt
      781 gcgcttggcg tatcgggtgt tcggcacctc ggggccagtg gtcgtatgga tacccggctg
      841 ggttgtcggc aacgtcgaca acatcgacga gcctggtagc ccatacgcac ccatcctcga
      901 tttagcttcg cgcgatatcc ggttggtggt gtgggacagg cggggcaccg gcctatcaga
      961 cccctccacg cacctgcttt cactggacga aaccgttacc gatctacgtg ccgtactcga
     1021 tgcagtcggt gacgatcagc cctcactatt tgggactggc gaaggcggcc cagtatcaat
     1081 tctgtacacc gcgacgtatc ccgaccgcat tggttcgctg gcgctctacg cggcggcagc
     1141 tcggttctca caggaattac cagattttcc atggggtttc acacccgacg aaatcgacgc
     1201 gcaactcgaa gatatcgaca accactgggg tgagggcgca ctgatcgagc tatttcatgg
     1261 cgcagcctct gactcgccgg gagtgcggga gctgttcggc aagctccagc ggtcggtgat
     1321 gagtccttcc tcggccaagc tctggtggcg cgccaccatg gaggtcgacg tccgcgcggt
     1381 tctgggcacc gtacgcgtcc ccaccttggt ccttgcgcgc caaggcgatg gactggtccc
     1441 gatcgaatcc gccatcgcgc tggcgacggg catcccaaac gcgcagtttc atgaactctc
     1501 ggcaggcgcg cacactccgt tcgacattct tcacgagctc gccgagcaga cgcggcggtt
     1561 ctttatcgac aagccagatg tcccggtcga agaacgtctc cttaaaacag tgatgttcac
     1621 cgatatcgtc ggctcaaccg aacaactcag cgcatcgggt gacgccaatt ggcgtcacca
     1681 gcttgatacc catgacaaag tcgtcgactg gctgttggag aagtacggcg gccaccgtgc
     1741 gaagcacacg ggagacgggg tcttcgcgct gttcgatggg ccgaccaagg cagctcgctg
     1801 cgcgctggat ctggtcccgg ccctcgccac gcgtggtatc cggattcgag tcggtattca
     1861 cactggcgag tgcgagcggc gcggcgatga gtggagcggg ctggctgtac acaccggcgc
     1921 tcgcatcggc gcattggcca gcacaggtga ggtgctggcc agccgcacgg tacgtgatct
     1981 ctctgcagga tcgggactga cgttcgaagg ccttggccaa caacgcctca aaggcctgcc
     2041 cgaggaagtc gatgtgtacc gagtcactgc aggttctgcg cgttagcgcg cagcaggatc
     2101 acgttgtcgg ccttggtcgt cggcgtcgcc aacgcgccga gggcggccat gttgcccgtg
     2161 tggtcgtcgg tgttgatcag gtgcgcgtag acggtcagcg tcgtcttcac gtcgcggtga
     2221 tccataagct ccgcgatgtc gatgggccgg atgcccgccg cgagacacaa gctggcgtac
     2281 gtgtggcgca gcgagtgcga ccgctcccag cggcacgccc caccagaaac ccggctcggt
     2341 caagaagtcg gacaccgcgc tcatgccccg cattctccca gcggctaccg acacaacccg
     2401 gcgtgtcgac gcgcgtgtcc gtttgccaat attgccctgc ggatagcgca cctgggtcag
     2461 ggtagttatt cgagaagtct tcacaacccc tcctcgacca tcacaatcgt gtagcgggac
     2521 acgttgccgt agttgggtgt tggtcgcgct gccaaagctc ccaaggcggc catgtcttcg
     2581 gtcgcgtcgt cgtcggaaaa caagtggacg tagacgctcg cctcagatca cggatgcatg
     2641 ggtagtcctg cggtcagtaa aatcacacag ccgacagtgg gtgggcggcc cggatcgcag
     2701 cggaactgca gtggccgcca cgcgcgaaac ggctccgatc gggccgagag tgtgcataaa
     2761 gttggcgacg acatgactga cgaagtccgg ccggatccag tgccgcgaga gcggcgacga
     2821 cgcggcgcgg ccgggttgtt ggacagcttc ctggactcgc cgatatcggg tttggcgccg
     2881 tggatcgtac tgtcggtgtt cgctggaccc ggtcacttca aagaagcgat ctctgcggtc
     2941 ttcggctttt cgttgctcat gttgtgggcg agccgtcgtc gtggggaccg gatgcattcg
     3001 ctgtcggtct tcggagtggt gtacttcggt gcgctgctac tggtcagagt gttggccggg
     3061 ccgggcaccg agtcctggct gagctcgtgg acgggcacga tgtccaatat cgcgttggct
     3121 gtgttcgcga cagggacgct gctgattcgt cgcccattta ccttgtcgta cgccaaagag
     3181 actacgcccg aagagttttg gggactgccc ggcttctatc ggaccaactt ggtcatctca
     3241 gcggtatggg cggcgtcgtt cgcggtcggc ggggtactcg gcgtcatcgg gaacgtggtg
     3301 ctgcacgact cggacaactt ctggaccgga tggattcttc agctcggagc caccttcttc
     3361 gcgatggctg ttaccggggt ctatcccgac tacgctggcg tcaagatgga gcgagaagcg
     3421 ggaatcacga tggaaccgct gccctcgctt ctgcccttca tcgactggtt gccgatgtac
     3481 gtgctcatca ccggcatcat cggctgggtc atcgacgaag ttcccgactg gctcgggatc
     3541 acgctcatcg ttgtcggcgc gataggtgtg gcagcgctgc gaaaattcgc gccatctgcc
     3601 gagcgcgagc cgtctggatg accacgccac gccgtcacga acgtccgtgt tcagtacagc
     3661 tggccttcct gacgaccttg gcgacgacgg cgatgtagtt caatggcaga acatcagctg
     3721 tgcacgctga atacgcggtt cgattcccgt catcggctcc acgaatccgc agttcggcgg
     3781 catattccgc gaccgccggc gcacatcaga attgatcttt tgaccgtctg accgtggcgc
     3841 ggggagtcag ccactgcaaa accgtttgcc gggcgctgag ccgggtggca ggtgtcgtac
     3901 gtctcccaga cttcgctgct tagttctcgg ccaggtatcg agagcactcg aaatccggct
     3961 cagacggatt ggctcgggat tgtgacgatc gtcacttcta acgcccgtct atgggatgcg
     4021 tagtcacgga agcctccccc gtactggggc gctttcaggg ttgaccgcta tcctcgcgca
     4081 ccgtaattgc cacggcaaga gtacggacac cgacgaagga gtcgcctcat cgtgcaggac
     4141 catctccccg cgcccgccat ctctcgatcg gtccgcgtgt tggggccggt cgttctattg
     4201 gttgcgttgg cgttgtacct ggccgcatat ctgcggtggc ccagcctcgc cggccaggtc
     4261 gacctgttgg tgtaccggtt cggagctgtg cgcgcctggg atggtcaaga cctgtactcg
     4321 gtggggatga ccggagattc acacacgatg ctgttcgact acacgccgtt cgcggcgctg
     4381 tgctttctcc cgctgatcgg gttgagccag ccggcccttc tcacccttgg tcttttggtg
     4441 aacattgcct gcgtgggtta catcgtccgc cggatgctca ctgctgcagg aatcacgacc
     4501 gtaaccggtc tgtgggggtt gacggcgctt ctgatggctc cgatcatctg gctggagcct
     4561 gtccggctat cgcttcagct cggccagatc aatttgatca tgatggcgat cgtcctcgct
     4621 gatgtgctgt cgcaaccgtc ccgcaggtgg agtgggatag gtatcgggat cgccgccggg
     4681 atcaaactga ctccggcgct cttcatcatc tttctcgtcg cgaccgggcg acgtcgcgag
     4741 gcagtcatcg ctggatcaac gttcatctcg accgtcgccg tcggtttcgt tctgctgccg
     4801 gccgattcga acgagttctg gctgcggggg cggtttcacg atgtacgacg gatctccagc
     4861 gatcctctgg cgaacaccag tctggccgga ctcctcgcgc ggctcaactg ggcgacgcgg
     4921 tgggagaccg cggccgcagt cgtattcgcg atcgtcgcca tcgcaatcgc cgcggtggcg
     4981 tggcgtcgcg gtcatttact cttgggcgtc gccatcgccg ggatgacctc ggccgccgct
     5041 tcacccttca gctggagcca ccactgggtg tggttcgcac cgcttcttgt ccacctcggg
     5101 cttctcgggt ccgtacaccg ctcgcgcgtc gcggcgctgg caatgtggac gcttgcggcg
     5161 gtcttcggcg ggtggttcat cgcatcgcga agctcgcctc cacaagcagg tctgatgtct
     5221 ttgcgccacc cggggacgtg gaaccagctt cttccggcgg cttacctttt cgcgcttact
     5281 atcgtgctcg tctgcagcat gtacacgtta tggcgggcac gacagcaccc agcggccgca
     5341 acgatcggca ttgacgcgat ggtggagggc agtcgaccaa gagcgagcat cggcccgctc
     5401 taaccgcgat catcagccgt tgtcgggctt ttcctggacg gcggcgctgc cccaatcgac
     5461 tacccaagct ggttcgcggg tgtcacatgt ttcgcggcgg cttgccgtgg acgctccgac
     5521 ccgattcaca aacgcggata tgcatttgag cccttcgcct cgccttgtgg ttcgatctcg
     5581 acgacaacgg gatgttattg gtggtcaacg caatacggaa tggacgggaa tcactcagtt
     5641 gcgccatgta tagcgtggca tcagcggcat tgtggcacag gaggatagcg gtgcttagcg
     5701 gcatgatcgc aggggtaggg ccgaccgtgg tgggatatcg gcatcacttc gaatgcgcga
     5761 actcagcgtc ccatcgatcc atgttctcta tcgcagtgtg aaacggtgcc ggaccgatgc
     5821 tttcctctcc cgtgaacggg cggtccatca cgacgatgag gaagaagacc agtcccatcg
     5881 acatcgcgag caacccgatg atccagaagc tgaagcggga cggcgagagc accgcagtcg
     5941 tcccgatcgt caacgccgag ccgatcaata cgaccatcca tagcgtgaag gggaccttcg
     6001 cgtcggcggc gtgaagccgc gttcgacgct cactcaggac atcatttgcc gctgaccaga
     6061 tctcgggcat gagcgcggcg cggcgcggcg tgtcgggttc gagttggccg acggcatgaa
     6121 acattcggtc gaacgcgtcc caggcctcag tgcttgattg tccccgacgc atatcgggcc
     6181 attccaccgt cacgacgacg tcggcgtact cgcggagagt cccgcgtgcc tctcgcgctt
     6241 gcgggctgtc gaacagcgac aaggctcgtg ccagttggcc gacgctgttg gattcgttga
     6301 ccacggccgt ttcggccgcg ccgaacgact gccagactga tacggcgacg aacgcgagca
     6361 gcagagcata gatggtcgca atgacggtca ggacggccat cgacacattc gtctccgcct
     6421 cggtgaacga ccgccgccag agccgatgga tgagaacgta tccggggaag acgagcgcga
     6481 cgaccgcgcc gacgatgcac acggccatca gccagttggg gaggtgataa aggaacagca
     6541 ttgcgcgggg gagcgtatca agagaccacc ccccgccgtg cccatctgac cggcagactc
     6601 gaaagccggt gacgcaacga gaaccggcac tcctcacgcg gccagtccgc tgatttgagc
     6661 tggaaaccgc tcttatcgag tggcagcggc cctttgcggg gatactcgga ctgtcgtctc
     6721 ggaaacgaga atctgcggcg taaccgagag gtccgagatg cgtaccccag gaccggatgc
     6781 acaagacggc aaccgcaacc gggcggctgc ccgctggcgc agcccctggc ttattgcgcg
     6841 gatcttattg gtgcttctgc tgtctgccgc gccgttactg gcgatctcgg cgcacgtgtt
     6901 cgggctcgca ccacagaaca ccacggcact gctggtgatc ttccctctca tcgtcgggct
     6961 cgcagtgctg atcgcgttct cgccgcatcg gatcgacatg gtcctcggtc gaggacttct
     7021 cgcgggaatg gtggcctgct tgttctatga cgcattccga ctcttctgcg tccacgtcct
     7081 cgcactgatg gacgatttca ttccggtgat ggggacgtgg atcaccggtg gttcgcacgg
     7141 cgcggccagc gctgtggtcg gctacatgtg gcgctacctc ggcgatggcg gagggcttgg
     7201 tgtcgccttc ttcgccatcg cactggccgt cggcttgggt cgacgctcga cgcggcccgt
     7261 cagggtggtg ctcgctgcgg tcggcttcgc tgtcttcgtg tgggcaggcc tggtagcgac
     7321 ggtcgtattg gctccgcggg gcgaggaact gatgttccgt ctgacggtat ccacgcttgc
     7381 ggtcacactg atcggacacg tgatcttcgg cctcgttctc gggctcggct ttctgcaagc
     7441 acgacgtgga tggtatggcc cggaattgga atggccgctg attctctcgg taccgacgcg
     7501 gttgagagcg cacaacgtcg cgcaggcaca ttcccgcgca cattctcgcg tggtgcgcac
     7561 ggtcgaagcc gatacgccca agccgctctg aggccgaaga ggattcgtcg acgctgatgt
     7621 tcggctaacg gggagctgag agaacctgaa cgacctcgct cagcggagca ggtgacgtcg
     7681 caatgtcgcc gacgagcgga tgcgaaagct gcacgatgag aaacgtgttg gcggccacca
     7741 gcgcgctcac ggttgccacc atcggatagt gcatggcggc attccccacc ccgtagatga
     7801 tcgagcagcc gaggaccagc ccgctcgtca ggaagatgac cacccacaat gaccaagggg
     7861 gtccggtgtc ggtgctggcg agcagcaccc gctccgtacg agccttactg aggtcgttca
     7921 ggttgctgaa tgaggttgcc aggaaggtct tttgagtgtc tgtctgcggc ttgacgtccg
     7981 tgtacgcgac atacaagcgg tgcagtgcct catccgcatc ggagacgcgc tggccgttgg
     8041 ccagtagggg ccactcggcg atcgccgctt tctcgtagtc cagcaggctg cgtcggatcc
     8101 ggtcgccgtc ggctttgtcg aatacataga tgtccctcgc cagctgcacg ccggccgctc
     8161 cctcgcttcg ggtctcctca tccgcgacgt tgacctgccc ccacatcgcg gaaacgacga
     8221 agccgatgaa gaacgcgtag acgaatccca cgacgccgta cacgaatttc gtgacgtcat
     8281 tatgggcccc atccttcagc cgcgggaacc gctttcggac gtagacctga acgagaaccg
     8341 atccgcccac gatgagaacg atgagaccgg tcaggagcag ccacgagggg acgtcgttga
     8401 cgagccagcg gctcaccggc cacactcctt gcgcttcgcc ctgccaatgc gcgcagcgag
     8461 caccatggtc ggcaccgacg acaccgtcac acctgggcca ctaagccgcc acgtcgagct
     8521 gttctccggt ggcgagtcgc ccacgtcgtt ctccttcgac cgcacgatga taatcgctga
     8581 caaccgaaac ggagttggaa acgagaagca ctacgcccgg gtgtcaccac gaggcgatct
     8641 gctgtagtgc caccggtggc acccccgcat cgcgcgccgc gaggtcattg cgccgattgt
     8701 tctgagtagc cgcggcgcag ggcaaagggc tgctagcgtc tgctgcagcg tgtcggcgtc
     8761 tctcgagcaa acgggaagcc accctggctc cgccgatgtc gaacgcaacg gatgaccggg
     8821 ggttcattga ggaatgccac cggcggtccg ccccgctcga cgcaggtgcc gcggccgacc
     8881 acaaacccgc aacgctcccg agaccttctg ccgcagtggt ggtttcacgc gagtcagttt
     8941 tcggtgcagg cgtgacaccg ccgcggcgcg acgacgcccc tgaaaagggc gttgctcaac
     9001 cgctcggtgc caccgtcact cccaccggtg tcaacttttc ggtgtattcc gagcatgcga
     9061 ccggtgtcac gctcctgctc ttcgactcgc acgaggcgag gacgcccgcc cgtgaagtga
     9121 cactcaccgc gcggaataac aagagctttc acttctggca ctgtcacctc agcggggtgg
     9181 gccccggcca ggtatatgcc tatcggatgg acgggccgtt cgaacccggc agcgccggcc
     9241 tgcgtttcaa tcgcaacaag gtgctgatcg acccgaatgc cttcggccac atcgacaacc
     9301 tctgggaccg ggagaccgca gtcgggcccg aggacaacgt cgccacgtcg atgcgaagcg
     9361 tggtgatcga cccgaccggc tacgactggg agggcgaccg cccgctcaac atcccgctga
     9421 ccgagaccat catctatgag gtacacgtgc ggggattcac caagtcgccc acgtcgaaag
     9481 tcgagcatcc cggttccttt cgcggcgtcg tcgagaagat cccgtacctg aagtcgctgg
     9541 gaatcaccgc ggtcgaactc ctaccgatct tcgacttcga cgagtcgcag gtcctccgca
     9601 gcgggccgac aggtcagtcc ctgaccaact actggggata cgacccgtac tgccattggg
     9661 ccccgcagtc gactttctgc atctccccac acctcggcac gcatctcgac gagttccgcg
     9721 acatggtcaa ggcgctccac cgcgccggca tcgaagtcat cctggacgtg gtcttcaacc
     9781 acacgggcga gggaaatcag aatgggccga ccatcagctt tcgtggtcag gccaacgagg
     9841 cctactacat gctctcgaaa gacgaccggc agtactacat ggactacacc ggggtgggca
     9901 acaccttcaa tgccaaccac ccggtggtca ccaagtacat cgtcgaatgc ctggagtact
     9961 gggtacgcga gcaccacgtc gacggtttcc gattcgacct cggctctgcg ctgtcccgtg
    10021 gtcctgacgg ggccgagatg gccgtgccac cagtgctgtg gaacatcgag ctgtcccgag
    10081 tcctatggga tgtcaaactc atcgccgagg cgtgggatgc gggcgggctg tatcaggtcg
    10141 gccggttccc cggcaatcgt tggtctcagt ggaacggtcc gttccgggac tgcgtgcgta
    10201 agagcgtcaa atccgatccc gggctcgtcg ccacactcgc gacacgtatc gcgggcagta
    10261 gcgacctctt cgggccctcc ggtgagagac caaccaacag catcaatttc gtcacatgcc
    10321 atgatggctt cacgctcaac gacctcgtca gctacgacgc caagcacaac gaagccaatg
    10381 gcgaaggcaa ccgggacggc gccgacgaca acaacagctg gaactccggt ttcgaggggc
    10441 cgaccgcgga tggcgatctg gaacgcctcc gagaacgtca gatccgcaac ttcgccgcga
    10501 ttttgttact cagccgcggt gtacccatgt tcgtggcagg cgacgagttc cgccgtacgc
    10561 agttgggcaa caacaatgct tactgtcagg acaacgagct gagctggatc gactggaccc
    10621 tggtagagcg gcacgccgac ctggttcggt tctggacgaa gatgatcggc ttccgcaagc
    10681 ggttccccac cttccgccag ccggatttct tcaccggcga ggtcaacgtg cgcggactcg
    10741 ccgatgtgtc ctggcacggc agcgatttgg gcgagcccga ctggcacgat cccggttctg
    10801 cggtgctctc cttcaccctc gcgggattag aaggcgccga cgacgttcac gtcatcctca
    10861 acatgtcgtc acagggcatc gacttcgcgc tgcctggcat cggtggtcga cggtgggcac
    10921 gggcgctcga caccacgctc gcgtctccgg acgacatcgt cgatccaggt gaggaagtcg
    10981 tcgtcgaatc cgataaatat cacgccggcg aacgcagtgt cgtcgccctc atctcacaag
    11041 aggttcagtc atgaaggaca tgccgttcac cgtgtcggac accatcgccg gatatgtcgt
    11101 gtcatacgac gatgacgaaa actcgttcgt cctacggacg accgacgggc gtgaataccc
    11161 tgtccatctc accccgggca cgttcgcgag cgtcatccgc aacttcgggg aggactatca
    11221 cgatgcgggc agccaattcg aggaacttct ctacccgggc cgctacctat acgcctacgg
    11281 catctactac ccggagggcc gggaacgccg attcgaggtg aagtccatca tcttccccgg
    11341 tgtcgccggc caggagtaca tgttcgagaa acccgactgg tgggtcaacc aggcccgggc
    11401 cgtggcggac ttctacctgc gcggccaatt ccccgaccac aactacgatt ggcgcaacta
    11461 ccggaccaaa ctgacgcttt ccggcacgca cacacccgac tacgtcgccc ccgacttccg
    11521 tcaggagacc gacaccatat cccggatggt gtacgggctg gccaccgcct tcctccttac
    11581 cggggaggac cgcttcctcg aagcggccga aggaggcacc gaatacctgc gcaagaacat
    11641 gcgggaggtg cgggcggacg agggtgtggt gtgctggttt cacgccatcg acgtctcgcc
    11701 gaagggccaa cgcaagatct ccgcctcaga attcggcgac gacttcgaag cgatcgcgat
    11761 gtacgagcag atctacgccc tggcaggccc gacccaaacg tatcgcgtca cgggcgaccc
    11821 actgattctc gacgacatcg agaagaccat cgcactattc gatcgctact tccgcgaccc
    11881 tgaccaaggt ggctacttct cccacctgga accggtgacc ctggatccac gttcggaatt
    11941 actccggcac aaccgcgccc gcaagaactg gaactccgtg ggcgaccatg caccggcgta
    12001 tctggtcaac ctgctcttgg cgttgggccg caaggagcat gaggactttc tggccaccac
    12061 gggggagacg atcgccgcgc gattccccaa cgaggacagc cccttcgtcc aggagcgttt
    12121 tcacgaggac tggacgtcgg accgccacta caagtggcaa caggacaatg cggtggtcgg
    12181 ccacaacctg aagatcgcct ggaatctcat gcggatcaat aacatccgtc ccaatgcagc
    12241 ctacgtcgag ctggccaaga agatagccga gaccatgccc gccgtcggca gcgaccagca
    12301 gcgcggtggc tggtacgacg tcgtcgagcg cactctcaag cccgggcagg catggcatcg
    12361 attcgtgtgg catgaccgga aggcgtggtg gcagcaagag caggccatcc tcgcctacct
    12421 agttctggcg ggcacgttcg gggacggcga gtacctccgg cacgggcgcg aatccgcgtc
    12481 cttctacaac gctatgttcc tcgatcacga cgatggtggc atctacttca atgtcctcgc
    12541 caacgggctg ccctacctgg tcggggatga gcggctgaag ggcagccact cgatgtcggc
    12601 ctaccattcc atcgagttat gctatctcgc agcggtttac accaatctgc tcatccgcga
    12661 ccaagccatg gaattgcact tcaaacccaa ggcctcatcg ttccccgacg ggatcctgcg
    12721 ggtggcaccc gaccttcttc cggcggggcg agtgatgctg tcgaacgttt gggtggacga
    12781 cgaaccctac gcggaattcg acaaggtcaa tctcaccgtg aaactaccca aagacaaagc
    12841 ggtacgcgtc cgggtcaggc tggtgtcgac gaacgccgag ttcgattcgc acgtccagca
    12901 gcgggaaggc gagtaccacc tgacgctgat aggcaagatt cccagaaaac gggtcaacca
    12961 gttgatcgca gaactcgaaa gggcgctgct cgccgatgcc acccgactgg tcctgatagt
    13021 caatgagttg gaatcgctct ccgaggacgc cctcagcgca ctcttcttgt tccggtccaa
    13081 ggtgaagttg gaacaggagg tcgtcatcgt gggcgccagt gacgcggtca tgagcacctt
    13141 cacgaactgt gattcgtcga cggggggaga gttcgtctac caggagacgc tctgaacacg
    13201 ggccggtgcg ctacgcggtg tctccggcca gcatgtcgat gatcttgcgg gcgaacaggt
    13261 ggaatacggt gcctgcgcgt cctgtcacga ggtcaccgtc gacgacgacg tctgcgtcga
    13321 cgtagatccc gcccatgttc ttcacatcgc cgaacaggtt gttgtgaacc accacacgcc
    13381 ggccacggac gagttcaggt gtcggtgcca gcagccacat tccgtggcag atgatgccct
    13441 tcacaatgtc aggtcgggcg aacacgcgcc gcatgaattc tgtagcaggc ggcagcttct
    13501 caacgtcgtc ggtgtagcgg agccgatccg acaccatccc cgacggcacg atgacggcgg
    13561 cgaactggcc cagattctcg tcgccgatgc cctcgaagct ctcgtcgcag cgcaacggcg
    13621 ccctcagttc gtgccccttg aactcgatcg agggctgtcc ccacaatcgg ctgagaaaat
    13681 gggcttcggc cccttcctcg gcgaaacgat gttggtagta atagatttcg ggctcataga
    13741 agtcgctctc cacgaggatc gcgatctttc tgccttgcag cctcatcgag tctccctgct
    13801 tccggaggcg ggtggcaccc tccgattctg cgcctccggt cgaatcgggg gctgtctcaa
    13861 ctcggctgtg gcaattggcc ctgcaccatc ttgatgaccg cgtgcagagg ctcgggcgtc
    13921 ggggcgaaca cgccgagata ggggtacgcc agttcgatga cgaggaagag gttcgcggca
    13981 acgaggacgc tgaccgccgt gaccaccgcg taatgccatc gctgttgctt cgcgccgaag
    14041 gcgacggcga aagcgagaac cagcccactg ctcagaaaga tgaccgccca taacgcaagt
    14101 ggcgggctga atcccgcgtc tgccgacagc agccgctccg tgcgcgcgtc gctcaactct
    14161 ttcaaggcgc gtagtgcgct catcaagacc acccgttgcc ggtcgtcgcc tggctcgaca
    14221 ttctggaccg cgctgtacag gccggacaag gcggtctccg cctccgccgt cggacggccg
    14281 ttggccgctt gcggccactc ggcaagtgcc gcttgctcgt attccatcag gctctgccgg
    14341 atccggtcac catcggcctt gccgaagacg ttgagttccc gcgtcatgga gatcgcggcg
    14401 gtcccctctg tgcgggccca ctcgtcctcg gttgccagct ggctcagcag gaacgcgacg
    14461 atgaacccga tgaagaagcc gtagacggga ccgaccacag acattccgac gtttgtgacg
    14521 tcgttgtgct tgccctcggc gagaccgggg aagcgacgcc ggacgaacgt caggccggca
    14581 atcgtcgcgg cgcaaatgca ggcgacgagg atcaccaaga tcagtgtcga cggaatattg
    14641 gtgacgatcc accggaccac tgcttcacac ctctcggcaa acgccccagc agactcgaca
    14701 gccgcatagc tgcgcctcgc tcatgtggcg gctgaagtcc gacgagcata acgcagccat
    14761 aaccgcgatt gcgcaaatca ccgtcatcac ttcgagatcc gttactgtcc gcgatcgtgt
    14821 cgcgcgtcga tgaacgcggg ctcgacgtcg cctacccctg gaacggcgag tggtctctgc
    14881 ggcagccggt gccgctcggt ggcaaaacgc cggtgaacag gtaatcgcgt ttgacgcgtt
    14941 cggtgcaggt actcgcgacg tacatgctgg tgtgccccgc gccttctgtc accaccacgc
    15001 gggcttcaga cagctgagac gcgcctgcgt acgctcccgg cagcggcgta gccgggtcgt
    15061 tcctcgtgtt gagcaccaga attggcgccg aggtccgacg attccatggg ccggtatagc
    15121 ggtcagggtc gtagccctgc cagaaggcgc acggcatcac gtcgaagacg gagatgcgcc
    15181 cgaagtccgg tgacgactca tcggccgtta ccgctgccag gctgtagctg gagggatccg
    15241 tcggcaccgt actgtcgacg cactggatgg cgtcgtaggc ttcttcgcgg ttgctcaggt
    15301 acgggtcttc gacgtcggtt tccggaggtc cgctgcctgt gacggcgtcg agcagttctg
    15361 gtgctgcggc accggtgtca gagatctcct gcagcaactg ggcaagctgc ggataggtgg
    15421 acggcttcgc cagcgtggcg acgatcatct ccggaacggt ccacatgtgg tcctcgacca
    15481 gaatcgttgt gcctcgggtg tgttcggcca acgctgccca tttggccacg ggatccccat
    15541 gcgagaaggc acatttcggg ccggccgcgc gacaatccct caagaactgg tcgaatgccg
    15601 cctccgtgcc gccggcgacg tcttgccggg tgttcaacgg gaccgtggtg ccctctgatc
    15661 cgtgaccgtt ggcgttgccg tcgaagtcga ttgatccgtc caggaccatc gcccgcacgc
    15721 gaccgggaaa caagttggcg taggtcgcac ccagcacaga gccgtaggac atcccgtggt
    15781 agctcaatgc cgggtcatcg acggcgcggc gcaacaactc gagatcgcgg gccgtgttcg
    15841 cagacgagac atggtccaga atcgggccgg cctgctgtct gcaacggtcg gcgagttcgc
    15901 cggaccaggc gaacagtttc tgctcaccgt cggcatcggc cggcagcccc ggactcggtc
    15961 tgaggaactg tgactgttcg gccgcgttcg aaaagcaccg cacccctgcg ctacgcgaca
    16021 ccccccgttg atcccacgaa acgaggtcga atcgcgaccg cagcgcgtcg gagaacagcc
    16081 acggccacct cgcgcgctga cgaagccggt caacgccgga ctgacccggc cccccgaagt
    16141 tgatgaacag ggttccgatc cgcttgctcg gatcgggcgc ggcaagtttg atcaccgcga
    16201 gagttatttg tgcgtcaccg gggcggtgat agtccaaagg gacttgtgct gtcgcgcagt
    16261 cgaatccatc agcacactcg acccagtcca aggtcggcgt gtccgccgct tcggcgagct
    16321 gatcgacata ggtgtcaggt gcgctggtgt cggcggcggt cggcgcaggc accaccgtgc
    16381 ctgcagcgac catgatggcc accgccagca ggcttgcgcg acctgagcga gatgagttca
    16441 ctggctgcca ggagatcttg tcagtccgaa gcgggcggga tgctctgctc gaaattgaac
    16501 acgttggtgg ggtcgtacct cgccttgatg ccgcgtagcc gcttgacatt gggcccccag
    16561 taagccttct cccagtccga cataccgacg ttcggcacgt tgacgtatgc gccgttcaca
    16621 tagggccgca acgcctggct gaattcagca atccacgcct gcgccgtgat ggtgacgggg
    16681 tcgctgtgag ccggcacccc gccgcccctg gtcccccagc cggcgcctat ttcgccatag
    16741 aagtttgcgg cgcggtgcgc atatgctgag ccgccggaag gctcgctgtc tttgaccctc
    16801 cccccgaacg gttggatgaa gtaattgcaa tcgggtgatg gtgccttcgc catgaaagac
    16861 ctgacgatgt cgatcgcctc agccgggaac gactcggtga tgtactgcga gaagaacttc
    16921 aagttcgcgg gctcccgcgg cgtcggaatc tggaagccag agtagatttc ggcccagggc
    16981 ccaattttca ccgccacctc cggcttaccg atcgacagaa gcggcgccaa cagttgcctc
    17041 gcctccgctt ccgtccccga tgcgagaact ccgaagagaa ggaccgagtc ggggtggacc
    17101 tccatctggc tagtgagacg atcgtcggta ttcggcgccg acgtctgcca ggcgtcgaac
    17161 acggcctgca catcgttgag tcccggccac gttgcggtca cgtaagcgac ctgcggcagc
    17221 ggatggacct tgtaggtcag cgaagtgacg atcccgaaat tcccatttcc ggctccgcgc
    17281 agagcccaca tgagatccgg atcgtttttc tcgtcaacgg agattgcctt ggccccatcg
    17341 gcacccgatg cgacgacgat ttcggcggcc atcagattgt ctgaggccat gccgaaggga
    17401 cggctgagga ggccgaagcc gccgccgagc gtagcgccaa ccaggccgac ggtgccctcg
    17461 gttcccgttg gtgcggcaag gccttccttg ccgagcgctg tcaccgcttc catctggttg
    17521 agaccggcgc cgacgatcgc cgtgccggcg accgtgtcga tcttggcgcc tttcatctcg
    17581 ctgacgtcga tcacgatgcc gttgtctacg ttgttccagc cttcgaggca gtggcggcca
    17641 ctgcgcacgc gaagagcgac gtcgttctgt cgcgcccacg tcagggcgtt gaccacgtcg
    17701 cgagtctcct gggcgaaaac gatcaccatg gggtagtgcg tgaagagctg atcccagccg
    17761 gcgctggacg ttacgtagtc cgggtcgccg ggacgaacga gacggccagt cagtttcgcc
    17821 ggcggactct tctcagcgac ggggtccgta ctcgaagacg tggcgccgga ggacgaattc
    17881 gaaccgctcg gctctgcgga cttggaagac gagtccgtgc tgcacgctgc gacgcccatc
    17941 agggctgcgg ccgcgccggt tgtgccgctg actataaggt tacggcgatt gagggtcatg
    18001 gcgcgcacgg tatcaagatc tcgaggccga attttcatgt tgctgaaatt gattcgtgtt
    18061 ccgctctgcg ttgtctcgca tgttcagtgt cgagccccgt ccccctctcg acgagaaagg
    18121 ctattcggaa cttgttgaaa gaaaatgcga ttcgcatctg ctgctcgcca cagcaggaca
    18181 agaagcccgc ggagtttcca gacccctcgc cattctccac attcggctcc actcggggag
    18241 cagccgtgaa aggattcaca cacaccgcga tcccgccggg cggtcgtggg ttcacggcca
    18301 gcccaaagga atccgattat gcgaacgacg atgaccggta tcggcgaccc tcccttgatc
    18361 ttcgtgcacg ggttcgcctg cgatggcacg gattggcagg ctcagcgcga gtcgctcgag
    18421 aagaagacca ccgtcattgt gtgcgagttg cccgggcatg gatttagccc gggcacatcg
    18481 gctgaatgca ccatcgaggc atacggcgct gcggtagcga aaacgttgag ggaactgccg
    18541 tcgcggccgg caatcctcgt gggccacagc atgggatgcc gtgtggtcct ggaggccaac
    18601 cgggtgcagt ccgacgccgt ggccgcgctg gtactcgtgg atggcagccg aatcagcagc
    18661 ggtgatccca tcgcggccga gcaggccatg gccgacgaac tggcagggga cggctaccgg
    18721 cgtttcgtgc gacagttctt cgaatcgatg ttcttccctt cgagcaaccc cgctctcgcc
    18781 atggcgatcg tcgagcgcgc gctgcgtttt cccacggggt tgggcaggac cctgatgacg
    18841 gacttggccg gctgggacgc ccgcgaaatg gagagcgccc tcgattcggt ccatgttccg
    18901 ctgctcgcta tccagagcac gacgctcgac accgcacgcc agcgggtatc actcgaaccg
    18961 ggcctgagct caccgtggct agaactggtc agcgcgcacg tgccgcaggc cagcatcgag
    19021 atgctgcacg ggtacagcca cttcccgcag atcgaactgg ccgatgaggt tacggccctc
    19081 atcgccgact gcgcttggcc ggccagccac cgatacgttc gtggacgagc ctgactcgcg
    19141 ggccgagcgc ccagtagtgg acccggcgtc gttcgaccct ggcgactgag cgagagctcg
    19201 gcacgagatt caacgaattc ataacgcgag aatcgtgcgt ggaaaccgcc gaaaatgaca
    19261 gcgccccccg ctcgaaagaa tcgaacccat caatgtggcg gaaacggctc acagtgggtg
    19321 gtcagggtat ttgattgagg tgctaactct caattattcg ttcttctcat ggagtaaata
    19381 tgccgattac aagtttcaca gctttttccg acgggctcga gccgcagcaa agaagccgcg
    19441 ctcacttcgt cgccgacacc gatctagacg atgatcgcct atgggttcca tggggagaaa
    19501 cagaaggcgt ttggtttcag ccgtgctgtt tcaatgtgac cgccggcggt ttcagtattg
    19561 tcgtgaaggc gcttccgggc gctgtgctcg gcgcccatta ccacgtgggc gacgtttatg
    19621 ggttcaccat gcgcggacat tggggatatc tcgaacatga cttcctcgcg acgccgggaa
    19681 catttatcta tgaacctgcg ggcgaggcgc acacgctctt tgtcgccaag gactccccgg
    19741 gaccgatgat gacattcttc gtcgtcgggg gtggcctcct cttcctggac aaaccggttg
    19801 atggcacctt cgagtcatac gaggacggtt tcaccttcct ggaaatggcg cgcaagcact
    19861 atcacgatat gggattggat gccgctcaac tggacgaacg tatccggtaa tcgtgaccgt
    19921 cacgtcgaac gggtttgcgc gacacatgct atgcggcttg cgaatcctag tggcgatctt
    19981 cggactgacc tcaatggcaa gctgcacgag cagttcgtcc gcaccagacc ctaatgcggg
    20041 aggttctgaa atcatcggca atcaacttcc ctccgctttg ttgggagtat ggactcgaga
    20101 atggatccga aggcccggtg tagcggttac tgatcaaaat gccgttcaga acagtccgct
    20161 agttgtcagg tatcttcaaa cgccagaatt tttcggagat atgcgcttcc cggtggaccg
    20221 gccggatctt tcccgcgcgt cgtcattcaa cgatttaaca gatgaggaac ttcgggcgct
    20281 gtcaaaacag caaggctttt ttggatatac cactgttgtc aaggattcta attccgggga
    20341 accttcgcga ccgccctcgg gtccaaccgt ccagtggcac cgtcaaatgg acttcgagcc
    20401 gccgacgagc aatctcgaca taggtcggct tgattttctt tcagcggatc taatgaatga
    20461 gagcgccccc gatgattcct acacggaacg ctgggtttct ataacgaacg gtgagaaccg
    20521 gttctttgtc gtctataccg agcatattgg gcggctcgac cgtatgttgc tggtagcggg
    20581 tgaccaattc ttctacggac gcaatcgcgc gaaggatctg ccgcccgccc cctcgcttga
    20641 ttcattgctg tccggagcca gtcgagggca gatgatcgag tacctggatc tagagttatc
    20701 ggtcgggcga gttcgcggag gattgtttcc gtgggagatt cagtattcga cacttccgtg
    20761 gcgggaggga aagcccgctg aattcgtcag atcgatccat gtcgatgatt ccaccaacaa
    20821 ggtgacgtta agtgtagggc gggacgacac ctggaagatg cccaccaaca ctttgaagcc
    20881 gacagactta agactgttgt ttcccgaaaa gtagcagccg gcagtagaag ggttttcggc
    20941 ggcggcacac tcacgctcgc cgtccctacc gccggccggc gatgaccgct ttcgtctccg
    21001 gatcgttctc gtcgcattgc gtgggaatcg tcccgttcat gtacccgccg ccgacattcc
    21061 aggactgctg cgcagcgtcc ttctccgaac cgcttgccac gcagttctgt tcgcgcgacc
    21121 gggcgattgc ttcgatgcac cgccaatagg agtaccgcgg atcagtcttg tctgcctcgg
    21181 tctccaccca cttgctgtat tcgtcgagat cggtgaagag cttgccgtcg atgatcgagc
    21241 cgtacatctc gaaacccttg gcgcacacca gtgtgttctc gaaggcgtcc gggttgttga
    21301 aaccggagta gcccttgacc ttggcttgga tgttgttgta cgaagtggtc ttgggaaagt
    21361 actgcgccag ttcattgagc gtgatgggct tgccatccgg cgcggtgggc ttctggatcg
    21421 tgagcgtgtt ggtgaacagc tgccaccgcc agtcgaagtt gttcacctgc cccacctgct
    21481 tgtcgtcggc gctcagcgaa ccgtcggcga tcgccttcgg caagaacttg tccttgaaca
    21541 gctgctgggg cacggaatac gtcgaagtcg ctccgttata ggacagttcg agcgtgacga
    21601 cctgcttgtc gttgtccaat gagtacttgg cgtgcaccga ggagtccggc gccgtcagcg
    21661 tgtcatccgg actacccagc gtgcgtattg cgaagccggg tggcgggccg ccggcgccgt
    21721 tgccgagcgc catcgtcgga taggccgtcc acccgtcgta ggtcgtgctg ttgaatcccg
    21781 actgccaagg cgagttccac ggtgtctgtg gaggcgcatc gccggggtcg aaacccgtac
    21841 tgccagcggt gagttcgaag ttgaatccgc cgttggccca cagcgtcttc ccgcctagaa
    21901 acccacagta agtcggcttg acatcgtcga atacgggtat cggctgcagg atcgtcattt
    21961 gatcccagag gctttgcgag ttgtggatcg gcggcaatgc actcttggtg accggcatgc
    22021 tgaacgagat ctcgtcgtaa ttggccttgg cgaacatgtt tggaccgtca tagaagcgaa
    22081 accacacggg cggcagcgct tcaccggcgc acttgtgcac ctcctgccgg tacgcatcgg
    22141 ccttgtgcgt agtggtgaag gtcctgtcgc cgacaatgct gaccgcgtcg ccctgagggg
    22201 tgatgcagac ggcggcctga ttctcgttct tcacctcgag ctgcggcact gcgcagagct
    22261 tggccaaata gccgccgtcc ctggcgccca acggctcaga agacggcgac ggagaggacg
    22321 actgcctaga tgaggatggc agcccggacg aggaacagct cactaatgtg aaggtcagaa
    22381 atggaatagc gaggaaagcc acgctggaac gtggggagaa ccggaaacgc atgaggtttc
    22441 ctcctgtccg gagtcgcctc cgccccgaat gtaacagccg gttgaatgcg cggatgagct
    22501 tttcatgaca tgtcgggagc tggatgtcga aaagtaagca gttgtgaacg gattgggtcc
    22561 ggctgacgat cggtaatacc gattattaaa gtaataccga ctcgagcacc aagtcgcgca
    22621 caaccgtctg atattcgccg tgagtgttgt gctcgattgt ctcgaactgc ttgccctgct
    22681 gctgccgcat gtattcgcgg tacttcggcg cgcctggaat ccggacatt
//
LOCUS       DASYLX010000995         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3137522,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000995 DASYLX010000000
VERSION     DASYLX010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137522"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGP27_23280"
     CDS             <1..601
                     /locus_tag="VGP27_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein serine/threonine phosphatase 2C family
                     protein"
                     /protein_id="HEV7854254.1"
                     /translation="VEGMLRALTASSRPPSAVLAGLTDAAMAAASAGLGSTDPNMAPS
                     CTYAAAVVVPTPDGVEITVGNVGDSRVYWLPEPPAAAQCLTVDDSLAQELISAGIAAD
                     SEAVQAGAHTLTRWLGADAEPTPGSDSSVWSITTAEPGLLLLCTDGLWNYLPDADDIA
                     RICAGDDADAAARALVDYALSAGGHDNVTVAVIPIGGPS"
     gene            598..1893
                     /locus_tag="VGP27_23285"
     CDS             598..1893
                     /locus_tag="VGP27_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7854255.1"
                     /translation="MSTAEQQSLSGIAVDVDHNPHLAEGTGTVDAIVSIAVGGDVVVD
                     KPPERVEAIIIDCSTSMLSPRDKFEEAKRATKAALDEMVDGTTFTIVAGTGKAGPVYP
                     IDGRPVQADDATRAAAAHAVDGLEPNGGTAIGTWLAYVRAIAEQHPGALTHAILLTDG
                     KDEHETPEELGEQIGLSEGEFTCDCRGVGTDWRVEELRAISSALLGTVDIVADPADLA
                     DDFAAMMRDSMNKSIPDLALRLWTPAGARVAFVKQVAPTVQDLTARRVEAETQTGEYP
                     LGAWGHEERDYHIQVEVEPADAGREKLAARVTVVAGDESLGQGLVTAMWTSDTALSAR
                     ISDRVAHYTGQAELAQAIQEGLAARNDGDTATATAKLGRAMTLARESGNDGTAKLLNR
                     VIEVDEHTGTARLRRDVAAADEMALDARSTRTARVRKEA"
     gene            1893..2510
                     /locus_tag="VGP27_23290"
     CDS             1893..2510
                     /locus_tag="VGP27_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV7854256.1"
                     /translation="MPTCPEGHDTAATDYCDVCGSPVTAAAPPATSKACPACGRAISG
                     RFCEACGHDSALAEPVAPKSADTEASVKWIAVVTADREYYKRVIARGGPDTVEFPEVF
                     PERRITLYDSTLIGRNNRKQGVEPGIDLGIHPIDRGVSTQHAVLQIRDSGPTITDLGS
                     TNGTSLNDSDHLLGNGEEIALSDGDRIHVGAWTTITVNKSEKRRT"
     gene            2507..>3949
                     /locus_tag="VGP27_23295"
     CDS             2507..>3949
                     /locus_tag="VGP27_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV7854257.1"
                     /translation="MTVDDRELHDAREQLAASREVLAALGRHAFDPGAVLDTVLEYAA
                     RLCDAAAAQLFLRDGEVFRLSRVTDETPDEYRSYLMAHPIARNRVSTVGRAAEDKQTV
                     QIPDVLADADYGRLDLQRLTGFRTLLSTPMILQDEVVGVLAMWRTDVAPFDERERRVL
                     EEFAVQGAIVLRQVDLMRSLESRGAELSDKVEQLEALREVDDAVGSSLDLDEVLERIV
                     TNAVRLTNLGFGDITLSTDGGSILEYDEATDSFHIRGKYGSGPALWERLRDITIDRRS
                     SLLGRAVLADQPLEIPDLAAAERDEFLDTVFQDGWRSVLAVPMIAGEEMIGVLVIRRR
                     GTGDFPSDVIELLETFASQSAVAIVNARLYRELETQSRELEIASNHKSEFLASMSHEL
                     RTPLNAVIGFSEVLLERMFGDINERQEEYLRDIWNSGRHLLELLNEILDLSKVEAGQM
                     MLDPSTFSVPSALEYTLAMVRERAAAHAIKI"
BASE COUNT          716 a         1345 c         1316 g          572 t
ORIGIN      
        1 agtggaaggg atgctgcgtg cgctcaccgc gtcgagccgg ccgccatcag cggtgctcgc
       61 gggcctgacc gatgccgcga tggcggcggc gagcgccggc ctcggcagca cagatccgaa
      121 catggcgccg tcgtgcacct atgcggcggc ggtcgtcgtc ccgactccgg acggagtcga
      181 gatcaccgtc ggcaacgtcg gcgacagcag ggtgtactgg cttcccgaac cgcctgcggc
      241 ggcacaatgc ctcacggtcg acgattccct tgcgcaggaa ctgatctccg cgggtatagc
      301 cgccgattcg gaagcggtgc aggcgggcgc ccacaccctt acccggtggc tgggcgccga
      361 cgccgaaccg acgccggggt cggactccag cgtgtggtcg atcacgaccg ccgagccagg
      421 cctgctgctg ctgtgcaccg acggtctgtg gaactacctt cccgacgccg acgacatcgc
      481 acggatatgc gcgggagacg acgcggacgc ggcggcgcgc gcactcgtcg actatgcgtt
      541 gagcgcaggc ggccacgaca acgtcaccgt cgcggtgatt ccgatcggag gaccttcgtg
      601 agtaccgctg agcagcagag cctctcaggc atcgccgtcg acgtcgacca caacccccac
      661 ctcgccgagg gaaccggaac ggtcgacgcg atcgtgagca tcgccgtcgg cggcgatgtg
      721 gtggtggaca agccgccgga gcgggtcgag gccatcatca tcgactgctc gacatcgatg
      781 ctctcaccgc gcgacaagtt cgaggaagcg aaacgggcca ccaaggctgc gctcgacgaa
      841 atggtcgacg gcaccacttt cacgatcgtg gcaggcaccg ggaaggccgg gcccgtctac
      901 ccgatcgacg gacgaccggt gcaggccgac gacgcgacga gggcggcggc cgcgcacgct
      961 gtggacggcc tcgagcccaa cggtggaacg gcgatcggca cctggcttgc ctatgtgcgt
     1021 gctatcgccg aacagcaccc gggagcgctc acccatgcga ttctgctgac cgacggcaag
     1081 gacgagcacg agaccccgga agagctgggc gaacaaatag ggctcagcga aggcgaattc
     1141 acctgcgact gccgcggagt cggcaccgac tggcgcgtcg aggagctacg cgccatttcg
     1201 tctgcgctgc tgggcactgt cgacatcgtc gccgatccgg ccgatctcgc cgacgacttc
     1261 gcggcgatga tgcgcgactc gatgaacaag tcgattcccg acctggcact gcggctatgg
     1321 actcctgccg gggcccgcgt cgcattcgtc aagcaggtgg cgccgaccgt gcaggatctc
     1381 actgcgcgac gagtcgaggc ggaaactcaa accggcgaat acccgcttgg cgcatggggt
     1441 cacgaggagc gtgactacca catacaggtc gaagtcgaac ccgcggacgc cggacgcgag
     1501 aagctggccg cccgcgtcac tgttgtggcc ggtgacgaat ccctcggtca ggggctcgtg
     1561 acggcgatgt ggacctccga caccgcgctg tcggctcgta tcagtgaccg cgtcgcgcac
     1621 tacaccggtc aggcagagct cgcgcaggcc attcaggaag gcctggcggc acgcaacgac
     1681 ggcgataccg cgaccgcgac cgcgaaactg ggccgcgcga tgacgctcgc cagggaatcg
     1741 ggcaacgacg gcacggccaa gctgctcaac agggtcatcg aggtcgacga gcacacgggc
     1801 accgcgcggc tgcgacgcga cgtcgccgcg gccgacgaga tggccctcga cgcgcgatcc
     1861 acccgaaccg cacgcgtccg gaaggaggcc tgatgccgac ctgccccgaa ggccacgaca
     1921 ccgcggccac cgactactgc gacgtctgcg gctccccggt caccgcggcc gcaccacccg
     1981 cgacttccaa ggcttgcccg gcatgcggca gggcgatcag cggccgcttc tgcgaggcgt
     2041 gcggccacga ttccgcgctg gccgaacccg tcgctccgaa gtcggcggat accgaagcgt
     2101 cggtcaaatg gatcgccgtt gtcaccgcgg accgggagta ctacaaacgt gtcatcgccc
     2161 gcggcggacc cgacaccgtc gagtttcccg aggttttccc ggagcgccga atcacgttgt
     2221 acgacagcac attgatcggc cgcaacaacc gcaagcaggg cgtcgagccc ggcatcgacc
     2281 tcggtatcca cccgatcgac cgcggcgtct ccacgcagca cgccgtcctg cagatccggg
     2341 attcgggtcc gacgatcacc gacctcgggt cgaccaacgg caccagcctc aacgacagcg
     2401 accatttgct gggcaacggt gaggagatcg cgctttctga cggcgaccgc atccacgtcg
     2461 gcgcctggac cacgatcacg gtcaacaagt ccgagaagag acggacatga ccgtcgacga
     2521 ccgcgaactg cacgacgcac gcgagcagct ggccgcgagc cgggaggttc tggcggcgct
     2581 cggccggcac gccttcgatc cgggcgcggt gctggacact gtgctcgaat acgccgcacg
     2641 gttgtgcgac gcggcggccg cccagttgtt tctgcgcgac ggcgaggtgt tccggctctc
     2701 ccgggtcacc gacgagaccc ccgacgaata ccggagctac ctgatggcgc atccgatcgc
     2761 gcgcaaccgg gtgtcgaccg tcggccgggc ggccgaggac aaacagaccg tgcagattcc
     2821 cgacgtgctg gccgatgccg attacggacg cctggacctg cagcgcctca ccggctttcg
     2881 caccctgctg tccacgccga tgatcctgca ggacgaggtg gtcggtgtac tggcgatgtg
     2941 gcgcaccgat gtggcgccct tcgatgagcg cgagcgccgg gtcctcgagg agttcgccgt
     3001 gcagggcgcc atcgtgttgc gccaggtgga cctgatgcgc tcgctggagt cacgaggcgc
     3061 cgagctgtcg gacaaggtcg aacaactcga agcgctccgg gaagtcgacg atgcggtcgg
     3121 gtcgagcctc gacctcgacg aggtcctcga gcgcatcgtc accaatgccg tgcggctgac
     3181 caacctgggc ttcggcgaca tcacgctgag caccgacggc ggatccatcc tcgaatacga
     3241 cgaggccacc gactcgttcc acatccgagg caagtacggc agcggccccg ccctgtggga
     3301 gcgactgcgc gacatcacca ttgaccggag gtccagcctg ctgggccggg ccgtgctggc
     3361 cgaccagccc ctcgagattc ccgacctcgc cgccgccgag cgcgacgagt ttttggacac
     3421 ggtgtttcag gacgggtggc ggtcggtgct cgccgtgccg atgatcgccg gcgaagagat
     3481 gatcggtgtg ctggtgatcc gccgtcgcgg caccggcgac ttcccgtccg acgtcatcga
     3541 gctactcgaa acgttcgcca gccaatcggc ggtcgccatc gtcaatgccc ggctgtaccg
     3601 cgagctggag acgcagagcc gagaactcga aatcgccagc aatcacaaat cggagttcct
     3661 cgcgagcatg tcgcacgaac tgcgaacccc gctgaacgcg gtgatcggct tctcggaagt
     3721 gttgctggag cgcatgttcg gggacatcaa cgaacgtcaa gaggagtacc tgcgcgacat
     3781 ctggaactcc gggcgccatc tgctggagct gctgaacgag atcctcgacc tgtccaaggt
     3841 cgaagcgggc cagatgatgc tcgacccgag caccttctcg gtgccgagcg ctctcgagta
     3901 cacgctggcg atggtccgcg agcgcgccgc cgcgcacgcc atcaagatc
//
LOCUS       DASYLX010000996         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1709778,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000996 DASYLX010000000
VERSION     DASYLX010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709778"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGP27_23300"
     CDS             complement(<1..596)
                     /locus_tag="VGP27_23300"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7854258.1"
                     /translation="MQIAIVLYPGFTALDFIGPYESLRMLPDTEVRFVWHQPGPITAD
                     SGVLVVGATHSFDETSSPDVVLVPGGFSTMEHARDEKVLDWLRSAHETATWTASVCSG
                     SILLAAAGLLQGKRATSHWAALSALKALGVDAVGDERIVHEGDVVTCAGVSAGIDLGL
                     WLAGQIGGEGRAKVIQLSMEYDPQPPFDSGHMSKASATT"
     gene            complement(607..>1540)
                     /locus_tag="VGP27_23305"
     CDS             complement(607..>1540)
                     /locus_tag="VGP27_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEV7854259.1"
                     /translation="VQALDVVGPFDVFTGATLNLAAQNRADDGYDVTIASVDGQQVAT
                     GTGLVLVAQPLPDPDGPIDTVVLPGGWGLDEARKNPALVDWVKAVYGTARRVVSVCTG
                     AFLAAEAGLLDGCRATTHWAFADDLAREFPSVTVDPDPIFVRSSPTVWTAAGVTAGID
                     LALALVEEDHGTEVAQTVARYLVLYLRRPGGQSQFAAPVWMPRAKRAPIREVQESIEA
                     EPAAAHSICDLARRASMSPRHFTRVFTGEVGEAPGAYVERIRTEAARRQLEETDDTVT
                     VIATRCGFGTAETLRRNFVRRMGISPDQYRKAFA"
BASE COUNT          250 a          544 c          507 g          239 t
ORIGIN      
        1 gtcgtcgccg acgccttcga catgtggccg gagtcgaacg gcggctgcgg gtcgtactcc
       61 atcgacaact gaatgacctt ggcacggccc tcgcccccga tctggcccgc cagccacagc
      121 ccgagatcga tcccggccga cacacccgcg cacgtgacta catcgccctc gtggacgatg
      181 cgctcgtcgc cgaccgcatc gacgcccaag gccttcagcg ccgacagcgc cgcccagtgg
      241 ctggtcgcgc gcttgccctg caacagaccg gcggcggcga gcagtatcga accggagcac
      301 accgacgccg tccacgtcgc ggtctcgtgc gcgcttcgca gccaatcgag caccttctcg
      361 tcgcgggcat gctccatcgt cgagaatcca ccgggcacga gaacgacgtc gggagaggat
      421 gtttcgtcga acgagtgcgt cgccccgacc accagcacgc cggagtccgc tgtgatcggg
      481 cccggctggt gccagacgaa gcgcacttcg gtgtcgggca gcatgcgcag gctctcgtat
      541 ggtccgatga agtccagcgc ggtgaagccg gggtacagca cgatggcgat ctgcatgatt
      601 gctcctctag gcgaatgctt tgcggtactg gtcgggcgaa atgcccattc ttcggacgaa
      661 attgcggcgc agggtctctg cggttccgaa gccgcaacgt gtggcgatga cggtgacggt
      721 gtcgtcggtc tcctccaatt gccgacgcgc cgcctcggtg cggatgcgtt cgacgtatgc
      781 gcccggcgcc tcgcccacct ccccggtgaa cacccgggtg aaatgccgcg ggctcatcga
      841 cgcgcgacgc gccaaatcac aaatgctgtg cgcggcagcg ggttcggcct cgatagactc
      901 ctgaacctcg cggatcggcg cccgcttcgc gcggggcatc cacaccggcg ccgcgaactg
      961 gctctgccca ccgggccgcc gcaggtacag cacgaggtac cgcgccaccg tctgcgccac
     1021 ctcggtgccg tggtcttcct cgaccagtgc cagcgccagg tcgattccgg cggtgacccc
     1081 ggccgccgtc cacacggtcg gcgaactccg tacgaagatc ggatcgggat ctactgtaac
     1141 ggaggggaat tcgcgggcga gatcatccgc gaaggcccag tgcgtggtcg cccggcagcc
     1201 gtccagcaat cccgcctccg ccgccaggaa cgcaccggtg cagacgctga cgaccctgcg
     1261 ggcggtgccg tacacggcct tcacccagtc gacgagcgcg gggttctttc gtgcctcgtc
     1321 caggccccaa ccaccaggga gcacgacggt gtcgatcggc ccgtccgggt cgggaagggg
     1381 ttgtgcgacg agcacgagcc cggtgccggt ggcgacctgc tgaccgtcga cggacgcgat
     1441 ggtcacgtcg taaccgtcgt cggcgcggtt ctgggcggcc aggttcagcg ttgcgccggt
     1501 gaacacgtcg aacggcccga ccacgtccag cgcctgaaca
//
LOCUS       DASYLX010000997         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1928641,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000997 DASYLX010000000
VERSION     DASYLX010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928641"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VGP27_23310"
     CDS             complement(<1..575)
                     /locus_tag="VGP27_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7854260.1"
                     /translation="MTRTDDDSWDITESVGATALGVAWARSQESTSQCPLFTDPFAEV
                     FVDEAIARGWQMPQRHVAERIRSIAGYAASRTKWFDEFFIAAGAGGIDQAVILAAGLD
                     ARAWRLPWNDGSVVYEIDQPKVLAFKTETLHSRGIEPATRYVAVPGDLRRDWPKALRE
                     AGFDPGEPTAWAAEGLLPYLPAAGQDLLFER"
     gene            complement(647..988)
                     /gene="mnhG"
                     /locus_tag="VGP27_23315"
     CDS             complement(647..988)
                     /gene="mnhG"
                     /locus_tag="VGP27_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEV7854261.1"
                     /translation="MNMPDIVTAVLVLSGSMLALTAAVGVVRFPDTLSRMHAATKPQV
                     LGLLLVLIGAAIRLRGNVDVGMLILTGMFTLITAPVVANRVGQLAYREQNVHDMLTTD
                     ELHEYTEGERE"
     gene            complement(985..1290)
                     /locus_tag="VGP27_23320"
     CDS             complement(985..1290)
                     /locus_tag="VGP27_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEV7854262.1"
                     /translation="MTPIWIIAAVLLTAAAAIVMFRILAGPSTLDRLVALDTLVAVTM
                     CAIGTWAAFSLDTTVTYGLTALALISFVGSVSVARFRVPGVGVGAHRYKRGESRYPR"
     gene            complement(1287..1820)
                     /locus_tag="VGP27_23325"
     CDS             complement(1287..1820)
                     /locus_tag="VGP27_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEV7854263.1"
                     /translation="MRRQVLRVWVLCWLTLVWVLLWGKLSAANILGGLAIALVITVLL
                     PLPAVPIEGRLHPLSLLRLVVVVAYQLVLSSVQVAWLAMKPGPPLLSAVLRARLAIKS
                     DLVLALAVNIMSLIPGSIVLEVDQERRLVYVHVIDVGNEKAVARFYRQVEQIERLLVA
                     AFERDADWQPSTDKETA"
     gene            complement(1817..>2810)
                     /locus_tag="VGP27_23330"
     CDS             complement(1817..>2810)
                     /locus_tag="VGP27_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV7854264.1"
                     /translation="ELATRLDGVASGTRSALFAVLLVAFGIKAAVFPLSTWLPDSYPT
                     APAPVTAVFAGLLTKVGVYAIIRAHTLLFPGGGLDRMLMVAAFLTMLVGIFGAIAQSD
                     IKRLLSFTLVSHIGYMVFGVALSSELGLSGAIYYVAHHILVQTTLFLVVGLIERQAGA
                     STLQRLGGLAAASPVLAFVFVVPALNLGGIPPFSGFIGKVALLEAGAQNGSVLAWLLV
                     GGSVVTSLLTLYVVARVWTLAFWRARADAPEGHLAAAAPSALLDDAEDIALNERGDVG
                     RMPVGMLAPTGALIVVGLTLTVLAGPIVSYSERAAAEVIDRNQYISAVLSEVRP"
BASE COUNT          514 a          998 c          889 g          409 t
ORIGIN      
        1 atgcgctcga agagcagatc ctgtccggcc gcgggtaggt acggcagtaa cccctcggcc
       61 gcccatgccg tcggctctcc cgggtcgaat ccggcctcgc gcaacgcctt cggccagtcg
      121 cgccgaaggt cgccgggtac cgccacataa cgggttgcgg gttcgatgcc gcggctgtgc
      181 agcgtctcgg tcttgaacgc cagcaccttc ggctggtcga tctcgtagac gacgctgccg
      241 tcgttccacg gcagccgcca cgccctggcg tcgagtcccg cggcgaggat caccgcctgg
      301 tcgatgccgc ccgcgcccgc cgcgatgaag aactcgtcga accatttcgt gcgcgacgcc
      361 gcgtatcccg ctatggagcg gatccgctcg gccacgtgcc gctgcggcat ctgccagccg
      421 cgcgcgatgg cctcgtcgac gaacacctcc gcgaacgggt cggtgaacag cggacactgc
      481 gacgtggact cttgagaacg cgcccacgcc acgccgagtg cagtcgcgcc gacactttcg
      541 gtgatgtccc aggagtcgtc gtcagttctg gtcattcgtt ccgccttcgg tgtactttgc
      601 tcggctcccg ctcgctccgg tcctcgctcg ttcctcgctg cgatcctcac tcccgctcgc
      661 cttcggtgta ctcgtgtagc tcatctgtgg tcagcatgtc gtgcacgttc tgttcgcggt
      721 atgccaactg cccgactcga ttggcgacga ccggcgcggt gatcaaggtg aacatgccgg
      781 tcaggatcaa catgccgaca tccacattgc cacgcagccg gatcgccgcg ccgatcagca
      841 ccagcagcag gccgagcacc tgaggtttgg tggctgcgtg catgcgcgac agagtgtccg
      901 ggaaccggac gacgccgacc gcggcggtga gcgccagcat cgacccgctg agtacgagca
      961 cggccgtgac gatgtcgggc atgttcaccg ggggtacctg ctctcgcccc tcttatatct
     1021 gtgcgcgccg acgccgacgc cgggaacgcg gaaccgcgcc acgctgaccg agccgacgaa
     1081 gctgatcaac gcaagcgcgg tgagtccgta ggtgacggtg gtgtccaggc tgaacgcggc
     1141 ccaggttccg atggcgcaca ttgtcaccgc caccaaggtg tccagcgcta cgaggcggtc
     1201 aagcgtgctc ggcccggcga ggatgcggaa catcacgatc gccgcggccg cggtcagcaa
     1261 gacggcggcg atgatccaga tgggggtcat gcggtctcct tgtcggtcga cggctgccag
     1321 tccgcatcgc gctcgaacgc cgccaccagc agccgctcga tctgctcgac ctgccggtag
     1381 aagcgggcaa cggccttctc gttgccgacg tcgatgacgt gcacgtagac cagtcgccgc
     1441 tcctggtcga cctccaacac gatcgagccg ggtatcagcg acatgatgtt gacggccagt
     1501 gccagcacca gatccgactt gatcgccagc cgggcgcgca gcaccgcgct gagcagcggc
     1561 ggtcccggct tcatcgccag ccaggccacc tgcaccgacg acagcaccaa ctgataggcg
     1621 acgacgacga ccaaccgcag cagcgacagc ggatgcagcc gcccctcgat cggtaccgcg
     1681 ggaagcggca acagcaccgt gatcaccaag gcgatcgcca ggccacccag aatgttggcg
     1741 gcggagagct tgccccacag cagaacccac accagtgtca gccagcacag cacccacact
     1801 cgcagcacct gacgtctcat ggcctcacct cggagaggac cgccgaaatg tattggttgc
     1861 ggtcgatcac ctcggcggcc gcgcgttcgc tgtaggacac gatcgggccc gccagcacgg
     1921 tcagcgtcaa cccgaccacg atcagcgctc cggtcggggc cagcatgccg accggcatcc
     1981 gcccgacatc gccacgctcg ttcaacgcga tgtcctcggc gtcgtcgagc aacgccgaag
     2041 gtgcggccgc cgcgagatgt ccctccgggg cgtcggcgcg ggcccgccag aacgccagcg
     2101 tccacactcg ggccaccacg tacagcgtca gcaagctggt caccacgctg ccgccgacca
     2161 gcagccacgc cagcaccgaa ccgttctgcg cgcccgcctc caacagcgca actttcccga
     2221 tgaacccgga aaagggcgga atcccaccca gattcagcgc gggtaccacg aagacgaacg
     2281 ccagaacagg actcgcggcg gccagtccgc cgagccgttg aagcgtggac gcgcccgcct
     2341 gacgctcgat caggccgacg acgaggaaca acgtcgtctg caccagaatg tgatgcgcca
     2401 catagtagat cgcgccggac agcccgagct cgctggacag cgcgaccccg aacaccatgt
     2461 agccgatgtg gctgacgagg gtgaacgaca gcagccgctt gatatcgctc tgcgcaatgg
     2521 cgccgaagat gccgacgagc atggtgagaa acgctgcgac catcaacatc cgatccagtc
     2581 cgccgccggg gaacagcagc gtgtgcgccc ggatgatcgc gtacacaccg actttcgtca
     2641 gcaggccggc gaacaccgcc gtgacggggg cgggcgcggt gggataggag tcgggcagcc
     2701 acgtcgacag cgggaacacc gccgccttga tgccgaacgc gacgagcagc accgcgaaca
     2761 gcgcgctgcg ggtacccgag gcgaccccgt ccagccgggt ggccagctcg
//
LOCUS       DASYLX010000998        12081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2477742,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000998 DASYLX010000000
VERSION     DASYLX010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477742"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGP27_23335"
     CDS             complement(<1..230)
                     /locus_tag="VGP27_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854265.1"
                     /translation="MAPRTNSLDPRAERVRGKLREAAFALAHERPVDRLTVGDIVARA
                     AVSRQVFYQHFDDRDDAIAYAFTAAFASAAGG"
     gene            complement(321..668)
                     /locus_tag="VGP27_23340"
     CDS             complement(321..668)
                     /locus_tag="VGP27_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7854266.1"
                     /translation="METATVVVTLLLAALFTFSSSIKLLGVPKSLAIRDHLGVSPIQW
                     RAIGVLELAGVAGVLIGLLWAPIGIAAAIGLALLSIGAVAFHMRAADRLSDTAPAVIG
                     IALAAATAVLHTI"
     gene            complement(739..1050)
                     /locus_tag="VGP27_23345"
                     /pseudo
     CDS             complement(739..1050)
                     /locus_tag="VGP27_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            1056..1187
                     /locus_tag="VGP27_23350"
                     /pseudo
     CDS             1056..1187
                     /locus_tag="VGP27_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            complement(1197..3164)
                     /locus_tag="VGP27_23355"
     CDS             complement(1197..3164)
                     /locus_tag="VGP27_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEV7854267.1"
                     /translation="MTKSLGLRLNLPDNGAVARSLLGVLVLTAAALLWGPAGAATSTA
                     AAGAIAGAIALQDNPLGRIPLVVVISVELGIAVLAGGLTAGYSLVFVIVIALWCFVAG
                     MQWAVGAPAGLLAGASGLLLVIQHPHPALAAITSALFAVVAGLTQAALIAAWPPRRWR
                     MEREALTKVYRSLSADARKLAAEPAAEVDTSQLLECKEAFSASDTQSGRRTSAYRDWH
                     ALPDQIAKTLTALGAKATGHDAIGDLLTATADMLAAIATQSRTARRDAEYALKGVDAA
                     VAAVAISDTPLAQRLSRQLHRADALRFGRRHLQDWTGTLKMAFEVAYGHLNLTSPISR
                     HAVRLAGAAASGTAIARFADVPAGYWIPLTVLLVTRPETAHTYTRCVGRVAGICVGVV
                     VASALTLLWEPSGLAAAILAVVFLVITYAVSMFGYIAVTAAFGATIVFLLDITDASSS
                     VSDLLFAVVIGGALAVLFHVLVPDDALIRLHQRSGELLKTEIDYAATVIKAFVHDLDH
                     STDALAAAWQRAFRARAAFEAAAGATRVESRELRRWLRSFRAALNAVTSSCTALEQSL
                     PPHPSAALSREFVLAVDDYVEALRGDPPTPATPWSIDTAQLAIADQQLRDAATHIVSD
                     DGAARVLVAEVGTITRSLAGIAADTEDVATP"
     gene            3431..4267
                     /locus_tag="VGP27_23360"
     CDS             3431..4267
                     /locus_tag="VGP27_23360"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEV7854268.1"
                     /translation="MSTAEERATQLDLVARLKSSYPELPDAPTPDMLDHHRITAYLKP
                     VHDVGGEPCAPMKYENKQYEQWEEETYIICETLGWRGIWLSEERRRMGNVDVGRAEYL
                     GLPYYGRWLLAVARILVEKHHIGLTELSERMAEVQERYAGGLDGKKLEAKPKREGDGS
                     GVKRNNHAEEAAGKGDPQMFAGQAGTPQFKVGDPVVVRELPVVFYTRTPAYVRGATGE
                     IESVAYESPAAEDETWDRPAKAEWFYVVRFIMADLWYGYTGTADDIISTEIPEHWLQA
                     AE"
     gene            4289..5008
                     /gene="scnC"
                     /locus_tag="VGP27_23365"
     CDS             4289..5008
                     /gene="scnC"
                     /locus_tag="VGP27_23365"
                     /EC_number="3.5.5.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase subunit gamma"
                     /protein_id="HEV7854269.1"
                     /translation="MSDHGHDHDHDHDRTVAPMVDEITDFEVLEIALRELCIEKGIFT
                     AEDHRRMTEFAEHIGITPAAHLVAKAWLDPEFKKLALAEPMTASKEVGVDWLHPTGWG
                     TPSDFTAFQILADTPTVHNVIVCALCSCYPRPILGNSPEWYRTPNYRRRLVRWPRQVL
                     AEFGLYLPDEITVHVQDSNQKHRFMVMPMRPEGTEDWTEEQLVEILTRDCLIGVALPK
                     AGVTTNAIVDTRPALHPGTGG"
     gene            5005..5370
                     /locus_tag="VGP27_23370"
     CDS             5005..5370
                     /locus_tag="VGP27_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase"
                     /protein_id="HEV7854270.1"
                     /translation="MTEADATTRRSRIAPLDEIVERNQVWSEMAAKWGVENPVPPWKT
                     SLDGMCDALDKAACDADIPDFKQRRDEEDELSATIYADLPYPESQLVSLAHSLVARGL
                     IDEAELRQRLASIRERLEA"
     gene            5397..6119
                     /locus_tag="VGP27_23375"
     CDS             5397..6119
                     /locus_tag="VGP27_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854271.1"
                     /translation="MEKVILTLRGADADGAWCARLRTQVAADLLDIGLPGLAINVRDA
                     EVRDSLMTLTTMDPPVVAVVSVWTQQSYGAQLIATTARLRKECEDVAAYLVTESVPLP
                     PPATVPGQRTAGLANVALLRRPGDIDEAIWLSRWHIDHTPVAIETQATFGYTQNAVVR
                     PLTDGAPAISAIVEELFAAEAISDLHAFFGAADDDDLRDRMERMIASTAAFGANQNVD
                     TVPTSRYVYRSPFDGGRQKVNR"
     gene            6116..6559
                     /locus_tag="VGP27_23380"
     CDS             6116..6559
                     /locus_tag="VGP27_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEV7854272.1"
                     /translation="MTTLDEAVALARGESGLTVVSTLRADNTIQASLVNAGVLPHPGS
                     GSPVLAFVTYGRVKLANLRARPQLAATFRNGWQWATVEGYAELAGPDDPQPWLSDADQ
                     IRLLLRDVFAAAGGTHDDWDTYDRTMAEQRRTAVLVAPTRVYSNG"
     gene            6576..7370
                     /locus_tag="VGP27_23385"
     CDS             6576..7370
                     /locus_tag="VGP27_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7854273.1"
                     /translation="MAGAREECKSVLQISDANRVRTLTLNRPDVLNAFDEALYQATAV
                     AIREAAEDAEVAVVLLTGNGRAFSAGNDLTEMQARITDPSMAEAGSHFTAMIEELTNF
                     PKPLICAVNGLGVGIGATILGYADLVFMSAQARLKCPFTSLGVAPEAASSYLLPKLVG
                     RQNAAWMLMSSEWVTADEALRMGLAWRVCEPDDLLAEARRHAEILSARPIASLMAVKQ
                     TIADPFRAGITAATERENIHFAALMGAAANADALAAFIDRRGSAGT"
     gene            complement(7410..7604)
                     /locus_tag="VGP27_23390"
     CDS             complement(7410..7604)
                     /locus_tag="VGP27_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7854274.1"
                     /translation="MFVCLCTGATSHAVSECVANGASTSKEVAAACGAGSDCGRCKRT
                     VRAIIEGHFANNGTPSAASH"
     gene            complement(7616..8248)
                     /locus_tag="VGP27_23395"
     CDS             complement(7616..8248)
                     /locus_tag="VGP27_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HEV7854275.1"
                     /translation="MSEFNLHSLILACDFRVGDIDRMWNWLKRHHDNLASIGAHHVVL
                     YTSIWEPGRVLVTIGIRHARSIRDVIRSPAVFEWFDIAGLDDIPPIFGGQVVEKIDLD
                     APSSEEHVGRIVVGVMSSVDDVSALMEKVHDGLDRFKRGGVRKIWVYRALDDGQEVMI
                     LQEIDNETNARQWIDHPDAAAEWMSNAGLGAYPTRFVGKFAHLMSIDSKN"
     gene            8487..8981
                     /gene="bfr"
                     /locus_tag="VGP27_23400"
     CDS             8487..8981
                     /gene="bfr"
                     /locus_tag="VGP27_23400"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEV7854276.1"
                     /translation="MQGDPDILKLLNEQLTSELTAINQYFLHAKMQENWGFTELASHT
                     RAESFDEMRHAETITDRILLLDGLPNYQRLFSLRVGQTVKEQFEADLAVEYEVVARLR
                     PGIIMCREKQDATSANIFEKILADEENHIDYLETQLELMGKLGEELYLAQCVSRPPGG
                     TAAP"
     gene            9107..11164
                     /locus_tag="VGP27_23405"
     CDS             9107..11164
                     /locus_tag="VGP27_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV7854277.1"
                     /translation="MTSPGTTAAVSDPESSSTGISFQRRNFIFVAVLLGMLLAALDQT
                     IVATALPTVVADLGGAGHQSWVVTSYLLASTIVTAIVGKLGDLFGRKTVFQASVLFFL
                     AGSVLCGLSGSMTMLVASRALQGIGGGAIMVTAMAVIGEVIPMRERGRYQGALGAVFG
                     VTTVIGPLLGGFFTDHLTWRWAFWINIPVAVVVLLVGAAAIPSLARTTKPVIDYAGIL
                     FVGLGASGLTLATSWGGGEYPWGSPVIIGLFVASVASLAIFVWVESRAAEPILPIRLF
                     ASPVFTVCCILGFIVGFAMLGALTFLPTFMQFVDGVSATTSGLRTLPMVAGMLITSIG
                     SGIIVGRTGRYKIFPVAGTAIMAVGFVLLSRMGAATPTWQQSVFLFVLGAGIGLCMQV
                     LVLVVQNTASFADLGVATSGVTFFRTIGSSFGAAIFGSLFANFLADRIGPALAASGAP
                     PRAAQSPQALHALPPEMAAPIVNAYADSLDMVFLCAVPVAFVGFVVSLFLKEIPLREM
                     EAVSATDLGEGFGMPSTESPDKILEVAIGRLMRNSPEIRLRSIAGQAGCESDIALLWG
                     LLQIYRQSQVFGSARLTDIAERLRVPREVIEPTFDRLIDGGYALRTGNQLWLTQAGVR
                     QVNCASAALANRIVEKLATSSAFDGRPDREQVEEALERIGHRVLVQRDWDDDKAVPVA
                     AAS"
     gene            11236..11718
                     /locus_tag="VGP27_23410"
     CDS             11236..11718
                     /locus_tag="VGP27_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7854278.1"
                     /translation="MSRIGDFADDDAAAWAVMSPDIGAGMLKFTRAVYTKNRLPMRVR
                     ELARMVIALDNECVVCQNTRDSEGIAAGVDEELYDHAAEWRTWPGYSAEERIAAEFAE
                     RFAADHTGLRDDEDFWRRAREQFSDELMADLALSCAMWLGMGRMLRTLDIGQSCKLTL
                     "
     gene            11741..>12081
                     /locus_tag="VGP27_23415"
     CDS             11741..>12081
                     /locus_tag="VGP27_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV7854279.1"
                     /translation="MAAMAAKPLAAAAIAQLESDGVATLIGTVVNPAGLTHAKTVPLR
                     KMGAFADPGLGASPVWHVFTIDQTGIASSGALGVVGDQRIRIDLGALRILGDGLAWAP
                     GSFYDQDGGPDP"
BASE COUNT         2209 a         3895 c         3929 g         2048 t
ORIGIN      
        1 atacccccgg cggccgacgc gaacgccgcc gtgaatgcgt acgcgatcgc gtcgtcgcga
       61 tcgtcgaagt gttgatagaa gacctggcgg ctgaccgcgg cgcgcgccac gatgtcgccc
      121 accgtcaacc ggtcaaccgg acgctcgtgc gccagcgcga acgccgcctc gcgaagcttg
      181 ccgcgaaccc gctcggcgcg gggatccaaa ctgttggtgc gtggtgccat acctgcacca
      241 taatccttgt cgtagacagt tggccacgat agcttaggta acctaagaca ccttgccgga
      301 ctggtgtgac ggcggtgact tcagatggtg tgcagcacgg cggtcgccgc ggccaatgcg
      361 atgccgatca cggctggagc ggtgtccgag aggcgatcgg cggcccgcat gtggaaggcg
      421 acggcaccga ttgacaacag ggccagtccg atcgccgcag cgattccgat gggcgcccac
      481 agcaagccga tcagcactcc ggcgacgcct gcgagctcga gcaccccgat ggcgcgccac
      541 tgaatcggtg agaccccgag atggtcccgg atcgccaggg acttggggac gccgagcagt
      601 ttgatgctcg acgagaaggt gaacagggcc gcaagcagca gcgtcacgac gacggtggcg
      661 gtctccacca acgcacccta cgactgacga gcacgcgagg agcagcgaat gccctttgcg
      721 gtcgtcggac catgccactc agtgacccgc gacgacgtcc gcctcgtcga tctcgaccgg
      781 atccttcgca cccggtagga acgagtaggc cacacacacg atcccgaaga acagatagcc
      841 gagcgcgacc tccggattcg ccgcccatgc gacctggggc gcgtggatca gaccgatgaa
      901 cgacaacgct gcgcccacgg cggacgcgat cgccgagtag aggaacttct tctcgaggat
      961 gaatgtcacc atggtgccga ggatgaggcc taccaggacg gcgccctcac cgagtgtctg
     1021 caggccttcg tagacgaccc cggcaccgtt gacacgccga ggcgcgaatc gaccgcacgg
     1081 agggtgtgaa gacctatgtc gtcggccacc tcgccgacga cgagggcgtc acggtcgaag
     1141 ccgagggagt gttcatcaca cccaagtggg ctcgcgacga gaactgagcc gctatctcag
     1201 ggcgttgcga catcctcggt gtcggcggcg atgcccgcaa ggctgcgggt tatcgtgccg
     1261 acctctgcga caagtacccg agccgcgccg tcgtcggaga cgatatgcgt tgcagcgtcg
     1321 cgtaattgct gatcggcaat cgccaactgt gctgtgtcga tgctccacgg ggtggcgggt
     1381 gtcggtgggt caccgcgcaa ggcctcgacg tagtcgtcca cggccagcac gaactcgcgg
     1441 ctcagcgccg cggacgggtg gggaggcagg ctctgctcga gcgccgtgca cgagctggtg
     1501 acggcgttca acgcggcgcg gaaagacctc agccagcgcc tcaactcccg cgactcgacg
     1561 cgggttgccc cggctgcggc ctcaaacgcc gcacgggcgc ggaaggcgcg ctgccacgcc
     1621 gcggcaagtg cgtccgttga atggtcaagg tcgtgtacga acgccttgat gaccgttgcg
     1681 gcataatcga tttcggtctt caacagctct cctgaacgct ggtgcagccg gatcagggcg
     1741 tcatcgggca ccaggacgtg gaacagcacc gcgagagcac cgccgatcac cacggcgaat
     1801 agcaggtcgc tgaccgacga tgatgcatcg gtgatgtcga gaagaaacac gatcgtcgcc
     1861 ccaaaggccg cagtcaccgc gatgtacccg aacatcgata ccgcgtaagt gatgaccaga
     1921 aagacaaccg ccaagatcgc tgcggccagg ccactgggtt cccacagcag cgtcagcgct
     1981 gacgcgacta cgacgccgac gcagataccc gcgacccggc ccacacagcg ggtgtaggtg
     2041 tgggccgtct ccggccgcgt cacgagcagc accgtcagcg gaatccaata gcccgcaggc
     2101 acgtcggcga agcgggcgat cgcggttccg gaggcggcgg cgcctgcgag ccggacggca
     2161 tggcgcgata tcggcgacgt caggttgaga tgcccatagg ccacctcgaa cgccatcttc
     2221 agcgtgcccg tccagtcttg gagatgacgc cgaccgaagc gcagtgcatc tgcgcggtgg
     2281 agttggcgtg aaaggcgctg cgccagtggg gtgtccgata tcgccaccgc ggcaaccgcc
     2341 gcatcaacgc ccttgagtgc gtattccgcg tcccgtcgcg cggttcggct ttgggtggcg
     2401 atcgctgcga gcatgtccgc cgtcgccgtc aacaggtcac cgatcgcgtc atgtccggtc
     2461 gccttggcgc ccaacgccgt caacgtcttt gcgatctggt caggcagcgc gtgccagtcc
     2521 cggtatgccg atgtacgacg acccgactgg gtgtcgctgg cgctgaacgc ttccttgcac
     2581 tccaacagct gcgacgtgtc gacctccgcc gcgggctcgg cggccagctt tcgagcgtcc
     2641 gcactcaacg accggtagac cttggtcagt gcttcacgct ccatccgcca ccgccgcggt
     2701 ggccaagccg cgatgagtgc ggcttgtgtc agaccggcga cgacggcgaa gagggcagag
     2761 gtgatcgcag ctagggctgg gtgtggatgc tggatgacca acagcaggcc tgacgccccg
     2821 gcaaggaggc ctgcgggtgc accgactgcc cactgcatgc ctgcgacgaa acaccacagc
     2881 gcaatcacga tcacgaatac caggctgtag cccgcggtca gcccgccggc cagcactgcg
     2941 atgcccagct cgacggagat cacgacaacg agcgggatcc gaccgagcgg gttgtcctgc
     3001 aatgcgatgg cgcccgcaat cgcaccggcg gcggctgtgg aggtcgcggc gccggccggg
     3061 ccccaaagca gggcagccgc cgtcagaacc agcacgccga gcaggctcct tgcgaccgcg
     3121 ccgttgtccg gcaggttcag tcgcaggccc agactcttgg tcacccgacc attctcaccc
     3181 gtccgaggtc acatccgggt caggctggca cggcgaggtc tcggcgatcg ggcccgattg
     3241 aaccgctccg caatgacatc ggaccttgcc ggccggaact cacccgaccc attcgatttt
     3301 cttccaccag caaccattcg gcgatagagt cagctaaccc cttcagatta caaagggaca
     3361 ctccacgaaa gtttgcaact gatgtcgcgt aagccggctc gacgtcggcc gcgcggacga
     3421 agggagtcgc atgagcacgg ccgaggagcg ggcaactcaa ctcgacctgg tggcccggct
     3481 gaaatcgtct tatccggagt taccggatgc accgacgccc gacatgcttg atcatcaccg
     3541 gatcaccgcg tacctcaaac ccgtccatga cgtcggcggc gagccgtgtg cgccgatgaa
     3601 gtacgagaac aagcagtacg agcaatggga ggaagagact tacatcatct gcgagaccct
     3661 cggctggcgc ggcatctggc tgtcggagga acgccgccgc atggggaacg tcgacgtggg
     3721 ccgggccgaa taccttgggc tgccgtacta cgggcgctgg ctgctggccg tcgcgcggat
     3781 cctggtcgaa aagcaccaca tcggcctgac tgagttgagc gagcggatgg ccgaagtaca
     3841 ggagcgatac gcgggcgggc tcgacggcaa gaaactggaa gccaagccga agcgtgaagg
     3901 ggacggatcg ggggtcaagc gaaacaacca cgccgaggag gcggctggca aaggtgatcc
     3961 gcaaatgttt gcggggcagg ccggtacgcc gcagttcaag gtcggcgatc ccgtcgttgt
     4021 gcgcgagctt ccggtggtct tttacacccg caccccggct tatgtccgtg gcgcgaccgg
     4081 cgagatcgag tcggtggcct acgaaagtcc ggccgcggag gacgaaacgt gggaccggcc
     4141 cgcgaaggcg gagtggtttt acgtcgtccg cttcatcatg gccgacctgt ggtacggcta
     4201 cacgggaacg gccgacgaca ttatctccac cgaaattccc gaacactggc tgcaagccgc
     4261 cgaatgatcc gtccggagag gagtctgtat gtctgaccac ggtcatgacc atgaccatga
     4321 tcacgaccgc accgtcgcgc cgatggtcga cgagatcacc gacttcgagg tgctggagat
     4381 cgccctgcgt gaactgtgca tcgaaaaagg catcttcacc gccgaagacc accgccggat
     4441 gaccgaattt gccgagcaca tcgggatcac accggcagcg catctggtcg ccaaagcatg
     4501 gctggaccct gaattcaaga agctggcact ggccgaaccg atgacggcga gcaaggaggt
     4561 cggcgtcgac tggctgcacc ccaccgggtg gggcacccca agcgatttca ccgcgttcca
     4621 aatcctcgcc gacaccccga cggtgcacaa cgtcattgtg tgcgcgttgt gctcgtgcta
     4681 cccgcggccg atcctcggca actccccgga gtggtaccgg acaccgaatt atcgtcggcg
     4741 tctggttcgc tggccgcgtc aggtgttggc ggagttcgga ctctatctgc ccgacgaaat
     4801 caccgtccat gtccaggatt cgaaccagaa acaccggttc atggttatgc cgatgcggcc
     4861 cgagggtacc gaggactgga ccgaggagca gttggtcgaa atcctcaccc gggactgcct
     4921 cattggcgtc gcgctgccga aagcgggcgt gaccaccaac gcgatcgtcg acactcggcc
     4981 tgcactgcac ccgggaaccg gcggatgacg gaagccgacg ccacgacgcg ccgctccagg
     5041 atcgcgccgc tggacgaaat tgtcgaacgc aatcaggtct ggtcagagat ggccgccaag
     5101 tggggcgtcg aaaaccctgt gccgccgtgg aagacgagcc tggacgggat gtgcgatgcg
     5161 ctcgacaagg cggcctgtga cgcggacatc ccggacttca aacaacggcg cgatgaagag
     5221 gacgagctgt cggcgaccat ctacgcggat ctgccctatc cggagagcca actcgtctca
     5281 ctggcccact ctctggtggc ccgcggtctc atcgacgaag ccgagttgag gcaacggctt
     5341 gccagcatcc gcgagcggct cgaggcctga gagccccgat ccattaggtt cgggtggtgg
     5401 agaaagtcat cctcactctg cggggagccg acgccgacgg cgcctggtgc gccaggctgc
     5461 gcacgcaggt tgccgcggac cttctcgaca tcggcctgcc gggactggcg atcaatgtcc
     5521 gcgatgccga ggtgcgcgat tcgctgatga cactgacgac gatggacccg ccggtggtcg
     5581 ccgtcgtcag cgtctggacc cagcagtcct acggggcgca gctcattgcc acgacggcgc
     5641 ggctgcgcaa ggagtgtgag gacgtcgcgg cttatctcgt cacagaatcc gtgccgctgc
     5701 cgccgccggc gaccgtgccc ggtcagcgca cggccggtct tgccaatgtg gcactgctgc
     5761 gccgccccgg cgacatcgac gaagcgatct ggctgtcgcg ctggcacatc gaccacacac
     5821 cggtggcgat cgaaacccag gcaacgttcg gctacaccca gaacgcggtg gtgcggccgt
     5881 taaccgatgg cgcgccggcc atctcggcga tcgtcgagga actgtttgcg gccgaggcga
     5941 tctccgatct gcacgcgttc ttcggagccg ccgacgacga cgacctgcgc gatcggatgg
     6001 agcggatgat cgcttccacc gcagcgttcg gcgccaacca gaacgtcgac accgtgccca
     6061 ccagtcgcta cgtctaccga agcccgttcg acggaggaag gcagaaggtg aaccgatgac
     6121 cacactcgac gaagcggtgg ccctggcgcg cggcgagagc ggcttgacgg tggtgtcgac
     6181 cctgcgagcc gacaacacga ttcaggcgtc cctggtgaac gcgggcgtgc ttccgcatcc
     6241 cggaagcgga agcccggtgc tggcgttcgt cacctacggc agggtgaagc tcgcgaatct
     6301 acgggcccgc ccgcagctgg cggcgacctt ccgcaacggc tggcagtggg cgaccgtcga
     6361 gggctatgcc gaactcgccg gacccgacga tccgcagccc tggctctccg acgccgacca
     6421 gattcggctg ctgctgcgtg acgtgttcgc cgccgcaggc ggcacccatg acgattggga
     6481 cacctacgac cggaccatgg ccgaacaacg ccgcacggca gtgctcgtcg cacccactcg
     6541 ggtgtacagc aacggctgag ccttacgctt ctgctgtggc aggcgcacgc gaggaatgca
     6601 agtcggtgtt gcagatcagc gacgccaatc gggtccggac gctcacgctc aaccggcccg
     6661 atgtgctcaa cgcgttcgac gaggcgctct accaggcgac agcggtcgcg atccgcgagg
     6721 ccgccgagga tgccgaggtc gctgtcgttc tgctgaccgg caacggacgg gcgttcagcg
     6781 cgggcaacga cctcacggag atgcaggcgc ggatcaccga tccttcgatg gcggaggcgg
     6841 gcagccactt cacggcgatg atcgaggagt tgaccaactt cccgaagccg ttgatctgtg
     6901 ccgtcaatgg gctcggcgtc ggcatcggcg caacgattct cggctatgcc gatcttgtct
     6961 tcatgtcggc gcaggcgcgg ttgaagtgtc cattcaccag tctcggcgtc gcaccggaag
     7021 cggcatcgtc gtatctgctg cccaagttgg tcggcaggca gaacgcggcg tggatgctga
     7081 tgtcctcgga gtgggtcacc gccgacgagg cgctgcgcat gggattggca tggcgggtgt
     7141 gtgagccgga cgacctgctg gccgaggccc gtcggcacgc cgaaatcctc tcggccagac
     7201 cgattgcgag cctgatggcg gtcaagcaga cgattgcaga tccgttccgg gcgggaatca
     7261 ccgcggccac cgaacgcgag aacattcact tcgccgcgtt gatgggggca gcggccaacg
     7321 ccgacgcgct cgccgcgttc attgacaggc gggggagtgc gggcacctag cggcgggtgc
     7381 gtcgaacgat cgccgattac tgaactggac tagtggctgg cggccgacgg ggtgccgttg
     7441 ttggcgaagt gaccttcgat gatcgcccgg accgtgcgct tgcatcgtcc gcagtcggat
     7501 cctgccccgc acgcggccgc cacttccttc gatgtcgagg cgccatttgc gacgcactcg
     7561 ctcaccgcgt ggctggtggc tccggtacag aggcagacga acatctactg acccatcagt
     7621 tcttactgtc gatgctcatc aggtgagcga acttgccgac gaatcgcgtc ggataggcgc
     7681 cgagcccagc gttcgacatc cactccgcgg cggcgtcggg gtgatcgatc cactgccggg
     7741 cgttggtctc gttgtcgatc tcctgcagga tcatcacttc ctgcccgtcg tcgagggcgc
     7801 ggtaaaccca gatcttgcgc acgcctccac gcttgaagcg gtcgagaccg tcgtgcacct
     7861 tctccatcag cgccgacaca tcgtcgaccg acgacatgac gccgaccacg atccggccca
     7921 cgtgttcttc cgacgatggc gcatcgagat cgatcttctc gaccacctga ccgccgaaga
     7981 tcggcgggat gtcgtcgagt ccggcgatgt cgaaccattc gaataccgcg ggcgaacgga
     8041 tgacgtcgcg aatcgagcgc gcatgccgaa ttccgatcgt caccagcact cggccgggtt
     8101 cccaaatgga cgtgtagagg acgacgtgat gcgcccctat cgatgccaaa ttgtcgtgat
     8161 gacgtttgag ccaattccac atgcggtcga tgtcgccgac gcgaaaatcg caagcgagaa
     8221 ttagtgagtg caaattgaac tcactgatcg ttgtccttcg ttccgatgcg ctcgcaatca
     8281 tgtaagcgca gtctaacctt agttaggcca ggcagtccac cgccgcggcc cccgcattgt
     8341 caccgggctc cgaagatgtt gccggaaagc gcgaaacgcg ccgtacacgc tgcaggcgta
     8401 gttccgatcc gtcatgacat tgcactagat tggaggcgtc aagacagccc atcgaccgga
     8461 tgacgcgacc ggcgcaagga gccagaatgc aaggggaccc cgacattctc aaattgctca
     8521 acgagcagtt gacgagcgag ttgacggcca tcaaccagta tttcctgcac gcaaaaatgc
     8581 aggaaaattg gggattcacc gaattggctt ctcacactcg tgcggaatcc tttgatgaaa
     8641 tgcggcacgc cgagaccatt accgaccgga tcttgctcct ggacgggctg ccgaattacc
     8701 aacggctttt ctccttacgt gtcggtcaga ccgttaaaga acaattcgaa gccgacctcg
     8761 ccgtcgagta cgaggtggtg gccaggctga ggccggggat catcatgtgc cgcgagaagc
     8821 aagacgcgac gagcgcgaac atcttcgaga agatcctcgc cgacgaggag aatcacatcg
     8881 actacctgga aacgcagctc gagctgatgg gcaagctcgg cgaggagttg tacctggctc
     8941 agtgtgtttc caggccgccg ggcggtaccg ctgccccgta atgcgttgcg gaccgcgcgt
     9001 ttcgcgggtc gcccaagcgt gtaaccctgc cgagggtcgc cgtaacattc ctagcctgtc
     9061 taacgatatg gctgtggaaa acggttgtga tcgacgaaag gcaggcatga cgagtccggg
     9121 aacgacggct gccgtctccg atccggagtc gagcagcacc ggcatcagct ttcagcgccg
     9181 caacttcatc tttgttgcgg tactgctcgg catgctgctc gccgcgctgg accagacgat
     9241 cgtggcgacc gcgctgccca cggtggtagc cgatctcggc ggagccgggc accaatcctg
     9301 ggtcgtgaca agctatctgc tggcgtcgac catcgtcacc gccatcgtcg gcaagctcgg
     9361 cgacctgttc ggccgcaaga ccgtcttcca ggcgtcggtg ttgttcttcc tggccgggtc
     9421 ggtgctgtgc ggcctgagcg gttcgatgac gatgctggtc gcatcccgcg cgctgcaggg
     9481 catcggcggc ggcgccatca tggtgacggc catggccgtc atcggcgagg tcatcccgat
     9541 gcgcgagcgg ggccgctacc agggtgcgct cggtgcggtg ttcggcgtca ccacggtgat
     9601 cgggcccttg ctcggcggct tcttcaccga ccacctcacc tggcgatggg cgttctggat
     9661 caatattccc gtcgcggtcg tcgtgctcct cgtgggtgcc gcggccatcc cgtcccttgc
     9721 gcggacaaca aaaccggtca tcgattacgc aggcatcctg ttcgtcggcc tcggcgcatc
     9781 ggggctcacc ctggctacga gttggggggg tggagagtac ccatggggtt cccctgtgat
     9841 catcggtttg ttcgtcgcct ccgtcgcctc cctcgccatc ttcgtctggg tggaaagccg
     9901 tgcggcggag ccgattctgc cgatccgcct gttcgccagc ccagtgttca ccgtgtgttg
     9961 catcctcggc tttatcgtcg gatttgcaat gctcggcgcg ttgaccttcc ttccgacgtt
    10021 catgcagttc gtcgatggcg tctcagcgac cacctcgggc ctgcgcaccc ttccgatggt
    10081 ggcgggcatg ctcatcacgt cgatcggcag cggaatcatc gtcgggcgca ccggcaggta
    10141 caagatcttt cccgtcgcag gcaccgcgat catggcagtg ggattcgtgc tgctctccag
    10201 aatgggcgcc gccacgccga cctggcagca gtccgtgttc ctgttcgtgc tgggcgccgg
    10261 aatcgggctg tgcatgcagg tgctcgtgct tgtcgtgcag aacaccgcca gcttcgccga
    10321 tctcggtgtg gcgacgtctg gcgtgacttt tttccgcacg atcggaagct ccttcggcgc
    10381 ggccatcttc gggtccttgt tcgccaactt cctggcggac cgaatcgggc ctgcgctggc
    10441 cgcaagcggc gcgccgccgc gcgctgcaca gtcgccgcag gcgctgcacg cgctgccgcc
    10501 ggagatggcg gcgccgatag tgaacgccta cgcggactcg ctcgacatgg tgttcctgtg
    10561 tgctgtcccg gtcgcgttcg tcggcttcgt cgtctcactc ttcctcaaag agatcccgct
    10621 tcgcgagatg gaggcggtgt cggccaccga cctcggcgaa ggtttcggca tgccgagcac
    10681 cgaatcgccc gacaagatac tcgaggtcgc gatcggccgg ttgatgcgca actcaccgga
    10741 gatcaggctg cgcagcatcg ccgggcaggc ggggtgcgaa tccgacatcg ccctgctgtg
    10801 ggggctgctt cagatctacc gccagagcca ggtgttcggc tcggcgcggc tgaccgacat
    10861 cgccgagcga cttcgagtgc cgcgcgaggt tatcgagccg acgttcgacc ggctgatcga
    10921 cggcggctac gcgctgcgca ccgggaacca actctggctc acccaggcag gagtgcgaca
    10981 ggtcaactgt gcgtctgccg cactcgcgaa tcggatcgtc gagaagctcg cgacgtcgtc
    11041 cgccttcgat ggcaggcccg atcgcgaaca ggtcgaggag gcgttggaac ggatcggcca
    11101 cagggtcctc gtgcagcggg attgggacga cgacaaggcg gtcccggtcg ctgccgccag
    11161 ctgacctagc tgggggttcg atgccttttc tagaacgtgt tccactttct gtgtccccgg
    11221 cgtagcattc cggccatgag ccgcattgga gatttcgccg acgacgacgc cgccgcgtgg
    11281 gcggtgatgt cgcccgatat cggtgcaggc atgctcaagt tcacgcgcgc ggtttacacc
    11341 aagaaccggc tgccgatgcg ggttcgtgag ctggcgcgca tggtcatcgc gctcgacaac
    11401 gaatgcgtgg tgtgtcagaa cacccgcgac tccgagggca tcgcggcagg cgtcgacgag
    11461 gaactctacg accacgccgc cgaatggcga acctggccgg gctacagcgc cgaggaacga
    11521 atcgccgccg agttcgccga acgcttcgcg gccgatcaca ccggcctgcg cgacgacgag
    11581 gatttctggc ggcgggcacg cgagcagttc agcgacgaac tgatggccga tctggcgttg
    11641 tcgtgtgcga tgtggttggg catgggccgc atgctgcgca ccctcgacat cggccaaagc
    11701 tgcaagctca cgctatagct ctgcgcgcca cggaggcaga atggctgcca tggcagccaa
    11761 acccctcgcc gcagcggcaa tcgcccaact cgaatccgac ggggtcgcca ccctcatcgg
    11821 gacagtcgtc aaccctgccg gcctcactca cgccaaaacc gtcccgctga ggaagatggg
    11881 cgcgttcgcc gatccgggat tgggcgccag cccggtgtgg catgtgttca ccatcgacca
    11941 gacaggtatc gcgtcgagtg gcgccctcgg cgtcgtcggc gaccagcgca tcaggatcga
    12001 tctcggcgcg ctgcgcatcc tcggggacgg attagcctgg gcgccaggct ctttctacga
    12061 ccaggacggc ggaccggacc c
//
LOCUS       DASYLX010000999         8092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1709862,
            whole genome shotgun sequence.
ACCESSION   DASYLX010000999 DASYLX010000000
VERSION     DASYLX010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709862"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="ureG"
                     /locus_tag="VGP27_23420"
     CDS             <1..618
                     /gene="ureG"
                     /locus_tag="VGP27_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEV7854280.1"
                     /translation="TAGEPLRIGVGGPVGSGKTALVAALCRELRDELSLAVLTNDIYT
                     TEDADFLRRHAVLPDDRIAAVQTGGCPHTAIRDDITANLDAIEDLIDAHAALDLILVE
                     SGGDNLTATFSSGLVDVQVFVVDVAGGDKVPRKGGPGVTFSDLLVVNKTDLAPLVGAD
                     LGVMRRDAAAARGDRPTVLISLTDDPSAGPVKSWVRDQLRVAQVV"
     gene            619..1254
                     /locus_tag="VGP27_23425"
     CDS             619..1254
                     /locus_tag="VGP27_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEV7854281.1"
                     /translation="MHSDVLIVARPDRHPLIECAGGVAARATEPDTVHLVSTAATPLG
                     GDTIALRVIVEPGARLRVRSAAATVTLPGRSTAESTVHWHLEAAGDLDVDTEPTVVAG
                     ASRHAASTQLAVSGSGRVRLRERVQIGRTDECQGFWSGSLYADVDGKPLLRHRIELGE
                     ESAGHDLISAPLASVSELRYPDVGTDALGTVLELARGGSLATWQGQRLGAR"
     gene            complement(1335..2705)
                     /locus_tag="VGP27_23430"
     CDS             complement(1335..2705)
                     /locus_tag="VGP27_23430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854282.1"
                     /translation="MSHPGATPSDRHKVVIIGSGFGGLTAAKALKHADVDIKIIAKTT
                     HHLFQPLLYQVATGIISSGEIAPPTRMILRKQRNAQVLLGEATHVDLARKSVRSQLLG
                     HTYVTPYDTLIVAAGAGQSYFGNDHFAEFAPGMKSIDDALELRGRIIGAFEQAERSSD
                     PARREKLLTFTVVGAGPTGVEMAGQIAELSEYTLKGAFRHIDSTKTRVILLDAAPAVL
                     PPMGEKLGKMAQARLEKMGVEIQLNAMVTDVDRNGITVKDSDGTTRRIECATKVWSAG
                     VSASPLGKQMADQSTAEVDRAGRLKVAPDLSVPGYPSVFVIGDMAAVEGVPGMAQGAI
                     QGAKYVANTIKAELRGADPFEREPFEYFDKGSMATVSRFSAVAKIGPLEFGGFIAWLA
                     WLALHLVYLVGFRRKITTLLSWTVTFLGRARGNLYITEQQAYARTRIEELQEIAAAVE
                     GEKAAS"
     gene            complement(2814..3746)
                     /locus_tag="VGP27_23435"
     CDS             complement(2814..3746)
                     /locus_tag="VGP27_23435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854283.1"
                     /translation="MTIKLGLQINNFSYGTDVAELYPTVIAQAQEADTAGFDSLFVMD
                     HFYQLPGIGTPDQPMLEAYTALGGVATATSNLQLGTLVTGNTYRNPTLLAKAVTTLDV
                     MSQGRAILGIGTGWFELEHDQLGYDFGTFTDRFNRLDEALQVILPMIKGERPTFSGKY
                     YRTQDAMANPRLRDHIPLLIGGSGEKKTIPLATRFFDHLNVIAGFDELTRKVDVIKQN
                     CEKIDRDPATLETSMLIGAIFGDGVSADVIPAGMEQRIVAGASADDVAEQIKTKVLDA
                     GITGAIVYLPTQITGYQPGQITQLGKALKPLVEG"
     gene            complement(3813..4487)
                     /locus_tag="VGP27_23440"
     CDS             complement(3813..4487)
                     /locus_tag="VGP27_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854284.1"
                     /translation="MAMEVLVTGGDTDLGRTIAETFRDAGHRVVITGARRDDLEVAAK
                     ELDVDAIVCDNIDPASLEEARSLFPHHLDTIVNVPAPRWDAGDPRTYTLADHAAAWRH
                     ALDATILSTVLTMQVVGDHLRSGGSVISVVPENPPEGGADAAIKAAVANWTEGQAVHF
                     GTRGITVNAVASGRSAQEGYAGLGSSAPVAAEISRLALFLSTPAARHITGQTMHVSNG
                     ALAHLG"
     gene            4636..5403
                     /gene="modA"
                     /locus_tag="VGP27_23445"
     CDS             4636..5403
                     /gene="modA"
                     /locus_tag="VGP27_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7854285.1"
                     /translation="MTRSSPLAFVFALLVLVVGLVGCGSSDKGSANQSITVFAASSLK
                     KPFTEIGERFKADNRGSSVELNFAGSADLVVQLVQGASADVFASADSNNMVKAEQAEL
                     VEGKPVAFASNTLTIVTAPGNPKQVTSFADLTKPELSVVVCAAEVPCGAATEKVEKAT
                     NTVLQPVSEESSVTDVLNKVTSGQADAGVVYVTDALAAGDKVAAVNFPESSAAVNTYP
                     IAVLKGAPKPDVAKKFVDLVTGEYGQKVLAEAGFAKP"
     gene            5427..6236
                     /locus_tag="VGP27_23450"
     CDS             5427..6236
                     /locus_tag="VGP27_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854286.1"
                     /translation="MHPGPALPRWMYFPAAVGALFVMLPLVAIVAKVDLPQFWTLITS
                     ESSRAALLLSLKTAAASTLLCVLFGVPMALVLARNDHRAVQAIRPLLLLPLVLPPVVG
                     GIALLYAFGRLGLIGHYLDAAGIRIAFTTTAVVLAQTFVSLPFLVIALEGAARTSGCD
                     YDVVAATLGARPTTVWWRVSLPLLAPGLISGAVLAFARSLGEFGATLTFAGSREGVTR
                     TLPLEIYLQRETDADAAVALSVLLVAVAAVVVLALGTRRLRSGSAIAGGAP"
     gene            6248..6733
                     /locus_tag="VGP27_23455"
                     /pseudo
     CDS             6248..6733
                     /locus_tag="VGP27_23455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            6755..7318
                     /locus_tag="VGP27_23460"
     CDS             6755..7318
                     /locus_tag="VGP27_23460"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEV7854287.1"
                     /translation="MRRLLRDNLTREGRSAVLITHDVLDVLTLADRVLVLESGRIVET
                     GSAASVLAAPRSHFGARFAGVNLVSGTAGPDGALNTEWGAAWHGNPAAEVVVGRPAVA
                     VFPPSAVAVYRDKPHGSPRNTVEVVVAELDSRGPALRVRADEQPDGAPGLAADITAES
                     AAELRVAPGERVYFTVKTQEITVHPVP"
     gene            7505..>8092
                     /locus_tag="VGP27_23465"
     CDS             7505..>8092
                     /locus_tag="VGP27_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0050840 - extracellular matrix binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APA family fibronectin-binding glycoprotein"
                     /protein_id="HEV7854288.1"
                     /translation="MHEPDAIPEHRKRTRRVKTLAMATAAGATVVALALPSVAQADPE
                     PSPSPAPPPPAGNTFLQGPPADQNAPPPPADPAAPAPAAADPNAPAPAAADPNAPAPP
                     PAPEPGRVDNAAGGFSYVVPAGWKVADATRLSYGQALLTKAGPEGTTPAPATQPGQQG
                     AQPANDTSVLLGRLDMKLFAGAEADNGKAATRLASD"
BASE COUNT         1255 a         2681 c         2725 g         1431 t
ORIGIN      
        1 acagcgggcg agccgttgcg gatcggcgtc ggcggtcccg tcgggtcggg taagacggca
       61 ctggtggcgg cgctgtgtcg cgagctgcgt gacgaactgt ccctggccgt gctgaccaac
      121 gacatctaca ccaccgagga cgccgacttc cttcgcaggc atgcggtgct gcccgacgac
      181 cgcatcgccg cggtgcagac cggtggatgt ccgcacaccg cgatccgcga cgacatcacg
      241 gccaatctcg atgccatcga ggacttgatc gacgcgcacg ccgcactgga cctgatcctg
      301 gtggagtccg gcggcgacaa tctcaccgcc acgttttcgt ccggcctggt cgacgttcag
      361 gtgttcgtcg tcgacgtcgc cggtggcgac aaggtgcccc gaaagggtgg tcccggtgtc
      421 accttctccg acctgctcgt ggtgaacaag accgacctgg cgccgttggt gggggccgac
      481 ctgggcgtga tgcgccgcga cgccgcggcc gcgcggggcg accgtcccac ggtgttgatc
      541 tcgctgaccg atgacccttc cgccgggccg gtcaagtcgt gggtccgcga tcagctccgt
      601 gtcgcccagg tcgtctagat gcattccgac gtcctgatcg tcgcgcggcc cgatcggcac
      661 ccgctgatcg aatgcgcggg cggtgtggcc gcgcgcgcca ccgagccgga caccgtgcac
      721 ctggtgtcga cggcggcgac ccccctcggc ggtgacacca tcgcgctgcg ggtgatcgtc
      781 gaaccaggtg cccggttgcg tgtccgcagc gccgccgcga cggtgacgct gcccggtcgc
      841 agcaccgcag agtcgaccgt gcactggcat ctcgaggcgg cgggtgacct cgatgtcgac
      901 accgagccca cggtggtcgc aggcgcctcc cggcatgcgg catcgacaca attggcggtg
      961 agcgggtccg gccgggtcag actgcgcgag cgtgtgcaga tcggcagaac cgatgagtgc
     1021 caaggctttt ggtcaggatc gctatacgcc gacgtcgacg gcaaaccgct gttgcgacac
     1081 cggatcgagc tcggtgaaga atcggccggt cacgatctga tcagcgcgcc gctggcgtcg
     1141 gtgagcgaac tgcgttatcc agacgtcggc accgacgcac tcggcaccgt gctcgaactc
     1201 gcgcgcggcg gcagcctcgc gacgtggcag gggcagcgac tgggcgcccg ctagacagat
     1261 ggatcgtcgc ccggctagac agatggccga tctcctcaac gcggctcgta cctcaccgct
     1321 tgatcgtcgc ccggctagct cgccgctttc tcgccttcga cggccgcagc gatctcctgc
     1381 agttcctcta tccgcgtgcg ggcgtaggcc tgctgctcgg tgatatagag atttccacgc
     1441 gcgcgaccga ggaacgtcac cgtccacgac agcagcgtgg tgattttgcg ccggaatccg
     1501 acgagataga ccaggtgcag cgccagccac gccagccacg cgatgaaacc accgaattcg
     1561 agcgggccga tcttggccac ggcagagaat cgcgacaccg tcgccatcga ccccttgtcg
     1621 aaatactcga acggctcccg ctcgaaagga tctgcgcccc gcagctcggc cttgatggtg
     1681 ttggcgacgt actttgcacc ctggatggcg ccctgcgcca tccctggcac gccctccacc
     1741 gcggccatgt cgccgatcac gaagacgctg ggatagccgg gaaccgacag atcaggcgcc
     1801 accttgaggc ggcctgcccg gtccacctcg gcggtggact ggtcggccat ctgtttgcca
     1861 agggggctgg ccgacactcc ggccgaccac accttggttg cgcattcgat acggcgagtg
     1921 gtcccgtcgg aatccttgac cgtgatgccg ttgcggtcga cgtcggtgac cattgcattg
     1981 agctggatct cgacgcccat cttctccagc cgcgcctgcg ccatcttgcc gagcttctcg
     2041 cccatgggag gcagcaccgc gggcgcggcg tcgagcagga tgacccgggt tttggtcgag
     2101 tcgatgtggc ggaacgcacc cttgagggtg tattcggaaa gctcggcgat ctggccggcc
     2161 atttccacgc ccgtcggacc ggccccgacg acggtgaagg tgagcagctt ttcccgtcgc
     2221 gccggatcgc tggatcgctc ggcctgttcg aacgcgccga tgatgcggcc gcgcagctcg
     2281 agtgcgtcgt cgatcgactt catacccggc gcgaactcgg cgaaatggtc gttaccgaag
     2341 tacgactgcc cggcgcccgc ggcgacgatc agggtgtcgt aaggggtgac ataggtgtgc
     2401 ccgagcaatt gcgaccggac agacttcctc gccaagtcga cgtgggtggc ttcgcccagt
     2461 agcacctggg cattcctctg cttgcgcagg atcatccggg tgggcggggc aatctcaccg
     2521 gaggagatga tgccagtagc cacctggtag agcagcggtt ggaacaggtg gtgtgtggtc
     2581 ttggcgatga tcttgatgtc gacgtcggcg tgtttgagag ccttggcggc agtcaacccg
     2641 ccgaatcccg acccgatgat gactaccttg tgccggtcag acggcgtcgc tccggggtgg
     2701 ctcatcagct gttgctcctc gaagggcttc acgttttata cagcgtctac cgtagtcgcc
     2761 cccatcccgt cgagagcggc catacgggtg agatttgcca cgtaagagca tgtttaaccc
     2821 tcgacgagcg gcttcagcgc ctttccgagc tgggtgatct gccccggctg gtagccggtg
     2881 atctgggtgg ggaggtagac gatcgccccg gtgattccgg cgtcgagcac cttggtcttg
     2941 atctgttcgg cgacgtcgtc ggcgctcgcg cccgcgacta tccgctgctc catgcctgcg
     3001 gggatcacat cggcgctgac accgtctccg aagatcgcgc cgatcagcat gctggtctcc
     3061 agggtggccg gatcgcggtc gatcttctcg cagttctgtt tgatgacgtc gaccttgcgg
     3121 gtcagctcgt cgaatccggc gatcacgttg agatggtcga agaaccgggt tgcgagcggg
     3181 atggtcttct tctcgccgct gccaccgatc aacagcggaa tgtggtcgcg taggcgggga
     3241 ttggccatcg catcctgggt gcggtagtac ttgccggaga acgtcgggcg ctcgcccttg
     3301 atcatcggca ggatgacctg caacgcctcg tccaacctgt tgaaccgatc ggtgaacgtg
     3361 ccgaagtcat agccgagctg atcgtgctcg agttcgaacc agccggtgcc gatgcccaga
     3421 atggcgcggc cctgactcat cacgtccagc gtggtgacag ccttggccag cagcgtcggg
     3481 ttgcggtagg tgttcccggt gaccagggtg ccgagctgaa ggttcgacgt cgcggtcgcc
     3541 acgccgccca gtgcggtgta ggcctccagc atgggctgat caggcgttcc gatgccgggc
     3601 agttgataga agtggtccat cacgaacagc gagtcgaagc cggccgtgtc ggcttcttgc
     3661 gcttgcgcga tgacggtggg atacagctcg gcgacatcgg tgccgtagga gaagttgttg
     3721 atctggagcc cgagtttgat ggtcacgcct tcacgtaacc acaactaacc ggggcggtca
     3781 ccccgcttta gcttcgggcg aacgcgggaa tatcagccca ggtgagcgag cgcgccgttg
     3841 ctgacgtgca tcgtctgccc ggtgatgtgc cgagcggcgg gtgtgctcag gaacagcgcc
     3901 aatcgggaga tctcggccgc caccggcgcc gaactgccga gccctgcata gccctcctgg
     3961 gcactgcggc cggaggccac cgcgttgacc gtgatgccgc gggtgccgaa gtggacggcc
     4021 tggccctcgg tccagttcgc cacggccgcc ttgatcgccg cgtcggcgcc gccctcgggc
     4081 gggttctccg gaacgacgct gatgactgag ccgcccgacc gtaggtgatc gccgacgacc
     4141 tgcatggtca gcaccgtcga gaggatcgtc gcgtccaacg cgtggcgcca ggccgcggcg
     4201 tgatcggcca gcgtataggt gcgcgggtcg cccgcatccc accgcggggc gggcacgttg
     4261 acgatggtgt cgaggtggtg cgggaacagg ctgcgggcct cttcgaggct ggccgggtcg
     4321 atgttgtcgc agacgattgc gtcgacgtcg agctccttgg ccgccacctc gaggtcgtca
     4381 cgacgagcgc cggtgatcac cactcgatga cccgcatcgc ggaaggtctc cgcaatcgtg
     4441 cggcccaaat ccgtatcacc gccggtgacc agcacttcca tcgccattcc tcctgtcagc
     4501 gcgggatctc cgcgcgtcgt ggcggtgatc cgccgacgat gttactggac ggtagctatt
     4561 aggcgaaacc tgacgcgcgt atcggttgcg cgatgacatc gacccgtgct ggatcggtga
     4621 cctagggttt gaccggtgac gagatcctcc ccgctcgcgt tcgtcttcgc cctgctggtg
     4681 ctcgtggtgg ggcttgtcgg atgtggctcg tcggacaagg gctcggcgaa ccagtcgatc
     4741 actgtctttg cggcgtcctc gctgaagaag cccttcaccg aaatcggcga gcggttcaag
     4801 gcggacaacc ggggcagttc ggtggaattg aacttcgcag gctctgccga tctggttgtt
     4861 cagctggtcc agggcgcgag cgccgatgtg ttcgcgtcgg ccgactccaa caacatggtc
     4921 aaggccgagc aggctgagct ggtggagggt aagccggtcg ccttcgcgtc caacacgctg
     4981 acgatcgtta ccgcaccggg gaacccgaag caggtcacgt cgttcgcgga tctgaccaag
     5041 ccggagcttt cggtggtggt ctgcgccgcg gaggtgccgt gcggcgcggc aaccgagaag
     5101 gtcgagaagg ccaccaacac ggttctgcag cccgtcagcg aggagtcgtc ggtgaccgat
     5161 gtgctgaata aggtgaccag cggccaggcc gacgcgggcg tcgtttacgt caccgatgct
     5221 ctcgccgcag gcgacaaggt ggcggcagtg aacttccccg agtcctcggc cgcggtgaac
     5281 acctatccca tcgcagtgct gaagggcgcc cccaaacctg acgtggcgaa gaagttcgtc
     5341 gacctggtga cgggggagta cggccaaaag gtgctggccg aggcgggttt cgccaagcct
     5401 tgaccaccgg atttcgcgct cctcgcgtgc accccgggcc cgcgctgccg cgctggatgt
     5461 acttccccgc cgcagtcggg gcgctcttcg tgatgctgcc gctcgtcgcg atcgtcgcga
     5521 aggtggactt gccgcagttt tggactttga tcaccagcga atcgtcgcgc gccgcactgc
     5581 tattgagcct gaagacggca gcagcaagca cgctgctgtg cgtgctgttc ggcgtcccga
     5641 tggccctcgt gctggcccgc aacgaccatc gggcggtgca ggcgatccgg ccgctgcttc
     5701 tcctgccgct cgtactgccg ccggtggtgg gtggcatcgc gctgctgtac gcgttcggcc
     5761 ggctcgggtt gatcggccac tacctcgatg cggcgggcat ccggatcgca ttcaccacga
     5821 ccgcggtggt attggcgcaa acctttgtgt ctcttccgtt tctggtcatc gcgctcgagg
     5881 gtgcggcacg cacatccggc tgcgactacg acgtggtggc ggcaacgctc ggcgcccggc
     5941 cgaccacggt gtggtggcgg gtgtccctgc cacttctggc acccgggctg atctcgggtg
     6001 cggtgttggc cttcgcccgt tcactcggcg agttcggcgc gacgctgacg ttcgcgggct
     6061 cgcgcgaagg cgtcacccgc accctgccat tggagatcta cctgcagcgt gaaaccgacg
     6121 ccgacgcagc cgtcgccctt tcggtgttgc tggtcgccgt cgccgctgtc gtggtgctcg
     6181 cgctgggcac ccgaagactt cgatcggggt ccgcgattgc cgggggcgca ccgtgaccgg
     6241 cgtgcacgtg cgcgcggtcg tcgccgatcg tgacgtcgac atcgaattca ccaccactgc
     6301 aggcgaagtg ctggccgtcc tcgggccgaa cggcgccgga aagtcgacga cgctacatgt
     6361 gatcgccgga ttggtgcggc ccgacgaggg acatgtacga ctcggtggcc gcgtactcac
     6421 cgacacggcg tccggcgtat tcgtgccgac acacgctcgc cgcgtcggca tgctgttgca
     6481 ggacgcactg ctgttcccgc acctgaatgt cgcggccaac gtggcgttcg caccgcgcag
     6541 tggccatcgt gggcgacccc gccgagaagc ccgcgcaacg gcggagcact ggctggcgaa
     6601 ggtggacgcc gccggactcg cggcgcgcat gcctcggcag ttgtccggcg gccaggcgca
     6661 gcgggtggcg ctggcacgcg ctcttgcggc agaccctgac gtgttgctgc ttgacgaacc
     6721 cctcgcaggc ctcgacgtcg cgaccgcgac cgcgatgcga aggctgttgc gtgacaactt
     6781 gacccgagaa gggcgttcag ccgtgttgat cacgcacgat gtactcgacg tgctcaccct
     6841 tgccgatcgg gtactcgtct tggagtccgg ccggatcgtt gaaaccggtt cggcggcgtc
     6901 cgtgttggcc gcgccgcgga gtcatttcgg tgcgcggttc gcgggcgtga acctcgtcag
     6961 cggcaccgcc ggaccggacg gcgccctcaa caccgagtgg ggtgccgcct ggcacggcaa
     7021 ccccgctgcg gaggtcgtcg tcgggaggcc tgccgttgcg gtgttcccgc catccgccgt
     7081 cgcggtctat cgcgacaagc cacacggcag tcctcggaac accgtcgagg tggtggtcgc
     7141 cgaactggac agcaggggac cggcccttcg cgtgcgcgcc gacgagcaac ccgacggcgc
     7201 gccgggcctc gccgccgaca tcaccgccga atcggcggcc gaactgaggg tggcgccggg
     7261 ggagcgtgtt tacttcacgg tcaagacgca ggaaatcacc gttcaccccg ttccgtaaca
     7321 acggccgggc gcgcatcgat caaatcatcg aagctcgcgt tgcaccgcgc gagcagcttg
     7381 gcctgccagc aaatttcgtt gagggcgcta gcgtgccttt tgccgctccc agcaatgtca
     7441 cagttaaaca ctctaagacc gccccacact tgcgtcacgg cagcaacact gtagtttcgt
     7501 ctgcatgcat gagccggatg cgatccctga acaccgcaag cgcacgcgcc gtgtgaagac
     7561 gctggcgatg gccacggcgg ccggtgccac ggtggtggcc ctcgcgctgc cgtccgtggc
     7621 tcaagccgat ccagaaccgt cgccgtcgcc cgccccgccg ccacctgcag gcaacacctt
     7681 ccttcaaggc ccgccggccg atcagaatgc accgccgcct ccggccgatc ccgccgcgcc
     7741 tgcgcctgcg gccgccgatc ccaatgcacc cgcgcctgcg gccgcggatc cgaacgcgcc
     7801 cgccccgccg cccgcgccgg agcccggtcg cgtcgataac gccgccggtg gcttcagcta
     7861 tgtggtgccc gcgggctgga aggtcgccga cgccacccgg ttgtcctacg gccaggctct
     7921 gctgaccaag gcagggccgg aggggaccac cccggccccg gcgactcagc ccggacagca
     7981 gggtgcgcag cccgccaacg acaccagcgt tctgctcggc aggctggaca tgaagttgtt
     8041 cgcaggcgcc gaagccgaca acggcaaggc ggccacccgg ctggcctccg at
//
LOCUS       DASYLX010001000         5464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3026627,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001000 DASYLX010000000
VERSION     DASYLX010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026627"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..469
                     /gene="trxA"
                     /locus_tag="VGP27_23470"
     CDS             17..469
                     /gene="trxA"
                     /locus_tag="VGP27_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7854289.1"
                     /translation="MESEIVTCPHCGKRNRVPAAGPGKPRCANCHQWLPWIAAAGDED
                     FAEVVETSPVPVLVDVWATWCGPCRMVSPALEQLATERAGQLKLVKVDVDKAPAIAQQ
                     FAIQAVPTLLVLKNHEVLGRQAGAAPVAALRNWLDQALSASNAKEARS"
     gene            466..729
                     /locus_tag="VGP27_23475"
     CDS             466..729
                     /locus_tag="VGP27_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV7854290.1"
                     /translation="MTSATIDPHVAAIREVSPRSDGCEECLRLGTPWVHLRLCLTCGH
                     VGCCDSSPMRHARAHAHTVGHPIVQNMEPGPTWRWCYVHENYV"
     gene            722..2416
                     /locus_tag="VGP27_23480"
     CDS             722..2416
                     /locus_tag="VGP27_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854291.1"
                     /translation="MSEVSTDQRASSQLVETRDIDGAYPRLADDQIATLEAGGTRRPV
                     DTGETLVREGERSHDFFVILSGKVAVTTTDDAGNRRVIRVHGPGRFLGELGDLEGQPA
                     FYTAEVVEPGEVLVVPTEQVRSLVAHDPALSDLILRAYLSRRSLLIQDETGFRIIGSC
                     FDPDTVRLREFASRNRLPHRWIDLERDKFAERLLQRFGVSPQDTPVVIWAGEVLRNPT
                     NTELARRVGLSVPDTVPDEADVVVVGAGPAGLASAVYGSSDGLITAAMERMAIGGQAG
                     TSSRIENYLGFPGGISGADLAERAGLQAGKFGARIMVSAEAIGLESDSGHHLLRLADQ
                     ASVVARAVVLATGARYRKLAVPRIESFEGNGVYYAATFQEALMCGTGPVVVVGGGNSA
                     GQAAIFLATRVFRVYVMIRGDDLNKDMSRYLVDQIHQHPRVTVQLCTEVREVHGDDEL
                     TAVVAEDKRTGERHTIQTRALFVFIGADPNTSWLAGAIELDDHGYVSTGSAALYSDTH
                     GNQERTKRQPMILETSRPGVFAAGDVRSGSVKRVASAVGEGSMAIRQIHEYFGSRP"
     gene            2713..3249
                     /locus_tag="VGP27_23485"
     CDS             2713..3249
                     /locus_tag="VGP27_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854292.1"
                     /translation="MRISDRVEAMAVALAVTVALMAAPAAGAVGTAVHDARSRLYAEA
                     AQQRYRATAIVTSTGQTARAPQYAIITTTVHAHWHAKGVEHTGTFSWDRAVATGNLIE
                     IWVDTQGRRVNPPPRPWRAAFDAVFTAVMIWLGVVAAAAALVAVVRWWLDRLHDAAWE
                     REIRSLCGNDGGRTGTQP"
     gene            complement(3356..4270)
                     /locus_tag="VGP27_23490"
     CDS             complement(3356..4270)
                     /locus_tag="VGP27_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7854293.1"
                     /translation="MSLDMQSSLGGQGLDELVPSRYAVQVGDIEVLVISDGVLPITAT
                     TLATNADATEFGRWLDDRFLPPDVLDWPLNVVVVRSGGRTILVDAGLGVEFPGFPRAG
                     QTVHRLEAAGIDPAAVTDVVLTHLHMDHVGGLLTEGLKERLRPDLRVHLATAEAEFWE
                     SPDFSRTDMPQPVPDALRSIATRFLEEYRGQMRTFETEYEVAPGVLVARTGGHTPGHS
                     IVRLASGDDRLTFAGDAVFAPGFENPEWHNGFEHDPEEAARVRVRLLRELAATGEWLV
                     ATHLPFPSVCRVAAARDVFRCVPADWDY"
     gene            4464..5222
                     /locus_tag="VGP27_23495"
     CDS             4464..5222
                     /locus_tag="VGP27_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854294.1"
                     /translation="MKIVVVGGTGLVGSKVMHALMDHGHDAVAAAPSTGVDAVTGRGL
                     TGVMTGAAVVVDVSNSPSFEETAATDFFRASTTNLLDAEASAGVGHHVALSVVGTERL
                     APQSGYFRAKLLQEQLIGAGPIPHTIVRATQFFEFLGTIADSATVGDTVRLPAALIQP
                     MASIDVAAAAAGAATRDPAGGSIEVGGPEAFRLPDLITTALTARGDARHVRADPTAQY
                     WGVDVDERTLVPGDGAVLFDTRFEDWLVEAAARS"
     gene            5335..>5464
                     /locus_tag="VGP27_23500"
     CDS             5335..>5464
                     /locus_tag="VGP27_23500"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7854295.1"
                     /translation="MNLLGRDRECQTLDELIAEVRSGSKVLVLRGEAGVGKSALLDH"
BASE COUNT          951 a         1804 c         1822 g          887 t
ORIGIN      
        1 tgacggagga ggcgccatgg aatcagaaat cgtgacatgc ccgcactgcg gaaaacgcaa
       61 ccgggtcccc gcggcgggcc cgggaaagcc gcggtgcgcc aactgtcacc agtggttgcc
      121 ctggatcgcc gcggccggcg acgaggactt cgccgaggtc gtcgagacct ccccggtacc
      181 ggtgctcgtc gatgtgtggg ccacctggtg cggaccctgc cggatggtga gcccggcact
      241 tgaacagctc gccaccgaac gtgcaggcca gctgaaactc gtaaaggtgg atgtggacaa
      301 ggcgcccgcg atcgcgcagc agttcgcaat ccaggcggta ccgacacttc tggtcctcaa
      361 aaaccacgaa gtccttggac gccaggccgg cgctgctccc gttgccgcac tgcgtaattg
      421 gctagatcag gcattgtcag ccagcaacgc gaaggaggca cggtcatgac atcggcgact
      481 atcgacccac acgtcgcggc gatccgcgaa gtcagcccgc gctcggacgg ctgtgaggaa
      541 tgcctgcggc tcggcacacc gtgggtgcac ctgcggctgt gcctgacgtg cgggcacgtc
      601 ggctgctgcg actcctcacc gatgcggcat gcccgggcgc acgcgcacac ggtgggccac
      661 ccgatcgtgc agaacatgga gccgggcccg acatggcgtt ggtgctacgt ccacgaaaac
      721 tatgtctgag gtcagcaccg accagcgcgc ctcaagccag ctggtggaaa cccgcgacat
      781 agacggcgcg tacccgcgac tggccgacga ccagatcgcc accctggagg ccggcgggac
      841 ccggcggccc gtcgacacag gcgaaacgct ggtccgcgag ggtgagcgtt cccacgactt
      901 cttcgtcatt ctgtccggca aggtcgccgt caccacaacc gacgacgcgg gcaaccggcg
      961 cgtgatccgg gtacacggcc cagggcggtt cctgggggag ctgggcgatc tcgaaggtca
     1021 acccgcgttc tacaccgccg aggtggtgga acccggcgaa gtactcgtgg tacccaccga
     1081 gcaggtgcgg tcgctggtgg cccacgatcc ggcactcagt gacctcatct tgcgcgcata
     1141 cctgtcacgc cgctcacttc tcatccagga cgaaaccgga tttcggatca tcggctcctg
     1201 ctttgacccc gataccgtgc ggctgcgaga attcgcatcg cgaaatcggt taccacacag
     1261 atggattgac ctcgagcgtg acaagtttgc cgagcggctg ctgcagcggt tcggagtgtc
     1321 gccccaagac accccggtgg tgatctgggc cggtgaggtg ctgcgcaacc cgaccaacac
     1381 cgagctggcc cgccgggtcg ggctgtcggt acccgacaca gtgcccgacg aagccgacgt
     1441 cgttgtggtg ggggccggtc cggccggact cgcatcggcg gtgtatggct cctcggacgg
     1501 tctgatcacc gcggccatgg agaggatggc aatcggcgga caggccggga cgtcatcgag
     1561 aatcgagaac tatctcggat tcccgggcgg gatttcgggt gccgacttag cagaacgggc
     1621 aggtctgcag gccgggaagt tcggcgcgcg gatcatggtc tccgcggagg ccatcgggct
     1681 ggaatccgac agcggacacc atctgctccg actcgccgat caagcgtcgg tggtcgcccg
     1741 tgccgtcgtg cttgccacgg gagcgcgcta tcgcaagctc gccgtcccgc gaatcgaatc
     1801 gttcgagggc aacggggtgt attacgccgc cacctttcaa gaggcgttga tgtgcggcac
     1861 ggggcccgtc gtcgtcgtcg gaggtggtaa ctccgcgggg caggcggcga tcttcctcgc
     1921 cacgcgggtt tttcgggtct atgtgatgat tcgcggcgac gacctgaaca aggacatgtc
     1981 gagatatctc gtcgaccaga tccaccagca cccgcgggtg accgtgcagt tgtgtaccga
     2041 ggtccgggag gtgcacggag acgatgaact cacggcggtg gtggccgagg acaaacggac
     2101 cggcgagcgt catacgatcc agacccgcgc tctgttcgtc ttcatcggtg ccgatccaaa
     2161 cacctcctgg ctcgctggtg ctatcgagct cgacgatcac ggctacgtgt cgaccggctc
     2221 ggccgcactc tactcggaca cccacggcaa tcaggaacga acaaaacggc agccgatgat
     2281 cctggagacc agccgaccgg gtgtcttcgc agcgggcgac gtgcgtagcg gctcggtcaa
     2341 gcgggtggcg tccgccgtcg gcgaaggatc tatggcaatc cgacaaattc acgaatactt
     2401 cggctccagg ccctgaccct tgagtccctt gcgccagtat gcaaggacgg ctggcgaagg
     2461 caatgcgccg gcgtcgccgc tcgctcgagc taatcgctga acccgcgggg ccgaaaacga
     2521 taggcgggat ttgtatcgcc agaaccattc tcacttcggc ccgtttggct ctggtacccg
     2581 cggaaacacc aagcgacggt agacgtttcg tttcgagaag tgtcaccgaa tgttcgagga
     2641 ctggtaatgg agatattcac cctcgcccca gcgcgatggc ggacagtccg ggccttgggg
     2701 agtagccctc ttgtgcgcat cagcgaccgc gtcgaagcga tggcggtcgc cctcgcggtc
     2761 acggtcgcgc tcatggccgc gcccgccgca ggcgccgtcg gcacagcagt tcacgacgcg
     2821 cgcagccggt tgtacgccga agcggcacag cagcggtatc gagccaccgc aattgtgacc
     2881 tcgaccgggc agacagcgcg cgccccgcaa tacgccatca tcaccaccac ggtgcacgct
     2941 cactggcatg cgaaaggcgt cgagcacacc ggcaccttca gctgggaccg tgcggtcgcg
     3001 accggcaacc tgatcgagat ttgggtggac acccagggac ggcgagtcaa cccaccgccc
     3061 aggccttggc gagcagcgtt cgacgccgtc ttcaccgctg tgatgatctg gttgggtgtg
     3121 gtcgccgcgg cggctgcgct ggtggctgtc gtgcgatggt ggctggaccg ccttcacgac
     3181 gccgcttggg aacgtgagat caggagcctt tgcggcaacg acggcgggcg aaccggcact
     3241 caaccatgag gatcggtatg tcgacaggtt gccgctgatc ccagttcaag gccttgagcg
     3301 cggcgcgcgg cggcggaacg accaccgccg cgcgccgccg cattcgggcg aggagtcagt
     3361 aatcccagtc ggcggggacg caccggaaga cgtcgcgtgc ggccgccacc cggcatacgg
     3421 acgggaatgg caggtgggtg gcgaccagcc actcgccggt cgccgccagt tcccgcagga
     3481 gacgaactcg gacgcgggcc gcctcctcgg gatcgtgttc gaatccgttg tgccactcgg
     3541 ggttttcgaa tccgggcgcg aacacggcgt cgccggcaaa ggtcagccgg tcgtcgccgg
     3601 acgccaggcg gacgatgctg tgcccggggg tgtgaccgcc ggtgcgggcg acgagcacgc
     3661 ccggtgccac ctcgtactcc gtctcgaacg tccgcatctg gccgcgatac tcctcgagga
     3721 atcgggtggc gatcgatcga agcgcatcgg ggaccggctg cggcatgtcg gtgcgggaga
     3781 agtcgggtga ttcccagaac tccgcttcgg cggtcgccaa gtgcacccgc aggtccgggc
     3841 gcagccgttc cttcaggccc tcggtgagta gcccgcccac gtggtccatg tgcaggtggg
     3901 tgagcaccac gtcggtcacg gctgcggggt cgatgcctgc ggcctccagt cggtggaccg
     3961 tctgcccggc ccgcgggaag ccggggaact ccacgccgag tccggcatcg acgaggatcg
     4021 tccggccgcc gctgcgcacc acgaccacgt tcagcggcca gtcgagcaca tcgggcggta
     4081 gaaacctgtc atccagccac cgtccgaatt cggtcgcatc ggcgttggtg gccagtgtcg
     4141 tggccgttat cggtagcacc ccgtcgctga tcaccagcac ctcgatgtcg ccgacctgca
     4201 ccgcgtagcg cgacggaacc aactcgtcga ggccctgacc gccaagcgat gactgcatgt
     4261 ccaaactcat gtttctcctt agggccgccg taactgacgg catcgtgaac agacctttcc
     4321 tcgcgatcga acgcgggcgg acccgcgaac tcatccatct ccaagagaac tccggctaat
     4381 ggatgaattc gcgaccggcc atccgttctc gcttgcagga accgctgcac aatgcactcg
     4441 gtcaacacct gaaaggggta gagatgaaga tcgtcgtcgt gggaggcacc gggctggtgg
     4501 gctcgaaggt gatgcacgcc ttgatggatc acggtcacga cgccgtcgcg gcggcgccgt
     4561 caacgggcgt ggacgccgtt acgggcaggg gactcactgg cgtgatgacc ggtgccgccg
     4621 tcgtcgtcga tgtctcgaac tcgccgtcgt tcgaggagac cgcggccacc gacttcttcc
     4681 gcgcgtccac gacgaacctg ctcgacgccg aagccagtgc cggtgtggga catcacgttg
     4741 cgttgtccgt cgtgggcacc gagcggctgg caccgcagag tgggtacttc cgggcgaagt
     4801 tgctgcaaga acagctcatt ggcgccggac cgattcccca caccatcgtg cgtgccacac
     4861 aattcttcga attcctcggt accatcgccg attccgcgac cgtcggtgac acggtgcgac
     4921 tgcccgccgc gctcatccag ccgatggcgt ccatcgacgt tgccgcggcc gccgccggcg
     4981 ccgcgacgcg cgatccggcg ggcgggagta tcgaggtcgg cggacccgaa gccttccggc
     5041 tgcccgacct catcacgacg gctctgaccg cgcgcggtga cgcccgtcac gtccgcgccg
     5101 accccacggc gcagtactgg ggggtcgacg tcgacgaacg caccctggtt cccggtgacg
     5161 gcgccgtcct gttcgacacg aggttcgagg actggctcgt cgaggccgcc gccaggtcgt
     5221 agtaactcgc gtgtcggcgc ccggccccac ctgccaccgg gcggacgtcg agcactggcg
     5281 gccggtgccg gccaacactc gtggaagtca ccgccgtcac tatcatcgac gtccgtgaac
     5341 ctgcttggtc gcgaccgcga atgccaaacc ctcgacgagc tcatcgccga ggtgcggtcc
     5401 ggctcgaagg tgttggtgct ccgcggcgag gcgggcgtcg gaaagtccgc actactcgac
     5461 cacg
//
LOCUS       DASYLX010001001         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3026676,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001001 DASYLX010000000
VERSION     DASYLX010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026676"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGP27_23505"
     CDS             complement(<1..983)
                     /locus_tag="VGP27_23505"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HEV7854296.1"
                     /translation="MCGIVGYVGTRDARGVVVDALRRMEYRGYDSSGVALLDGAGGLT
                     VRRRAGRLANLEAALAETDSASLAGSTGLGHTRWATHGRPTDRNAHPHRDASGKIAVV
                     HNGIIENYTVLRQELEADGVEFASDTDTEVAVHLVAREYRKGDTASDFTQSVFAVLPR
                     LEGHFTLVFANADEPGTIIAARRSTPLVLGVGDGEMFVGSDVAAFIEHTRNAVELGQD
                     QAVVVNADGYRITDFEGNDADPSTDYREFRIDWDLSAAEKGGYEYFMLKEIAEQPAAV
                     SDTLLGHFVDGRIVLDEQRLSDQELREIDKVFIVACGTAYHSGLLAKYAIE"
     gene            complement(1069..1368)
                     /locus_tag="VGP27_23510"
     CDS             complement(1069..1368)
                     /locus_tag="VGP27_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854297.1"
                     /translation="MNKKLIGFATGVGVAAAMAVAALGMGTATANAAPSPATQAGFAE
                     WGPGWGPGPGPWRPGPPPPPPPAWGGGYDYGGWNNYGPPPPPCVSGPLGFVQVCA"
     gene            1560..2405
                     /locus_tag="VGP27_23515"
     CDS             1560..2405
                     /locus_tag="VGP27_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7854298.1"
                     /translation="MAATKKLFAALRRRGPHRVLRGDLSFAGLPGVVYTPESGYNLPG
                     IAFGHEWLTGVEHYEGTLEHLASWGIVAAAPDTEKGIAPSPLNLAFDLGTTLDIIAGV
                     RLGEGKISVHPNKLGLVGHGFGASTAVFAAAGMPAKPKAVVAAFPTVTTPRAEEAAAT
                     LKVPGLILTDPGDPMSLRSNAVELARVWDAATLRAMPKAKAGGLIEGRRLARVVGLPG
                     ADRGTQKMVRAVMTGYLLHVLAGDKTYRDFADPEAALPKATTMDPFADDPVDLENKVV
                     ALLKP"
     gene            2459..3499
                     /locus_tag="VGP27_23520"
     CDS             2459..3499
                     /locus_tag="VGP27_23520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854299.1"
                     /translation="MRTGIFLNYAGGFLEAVDEVVELEKVGVDIALVAEAYSFDAISQ
                     LGFLAAKTSRIELGSGVVPIYVRTPSLLAMTAAGLDYVSDGRFRLGIGTSGPQVMEGF
                     HGVPFDAPLGRTREVVEICRQVWRRENVEFDGKHYQIPLPHHRGTGLGKPLHLINHPV
                     RERIPITIAALGPKNVELTAEIAEGWQPVFFYPEKADDAWGDSLRAGMAKRDPQLGPL
                     DIMVSATLAIGDDVEDRLAWAKPQLALYIGGMGARGKNFYHNLATRYGFGEVADRIQD
                     LYLSGRKTEAIDAVPDDLVRHVSLIGPKGFVKERLAAYAEAGVTTLLVHPLTGDRRES
                     VRFVEELRELLP"
     gene            complement(3512..>4502)
                     /locus_tag="VGP27_23525"
     CDS             complement(3512..>4502)
                     /locus_tag="VGP27_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854300.1"
                     /translation="DKVAGAVTIDDRRQAQRAEWLTATRTVTTGAGDRAAASELIDQQ
                     VKPFVDNDIRADWLAAMQRAMAAVSAAFDAATSALTGDPTSPFEVPGDLGPTSVPPPP
                     SSASPAHLSGTEPAVWSSPAPAAAPAVGPSSAPVAPVPAAPVAAAPDLPAAALPAAAA
                     PATGPASATPAMPTMPPLGELGGGLPGAAGGLAGIGSQLADAISGLLDQPHDALPDPS
                     EVTNDAVDEDKPAPDENEPAKDEEDEQTEGGPDVETAELAEEGEPTAEPVDACGHDEP
                     VDEAPQPDPVPTPPPAPMPPPPEPLVESPPDAAPVGAETPCEIAADELPQAGP"
BASE COUNT          680 a         1514 c         1554 g          754 t
ORIGIN      
        1 tgctcgatcg cgtacttggc cagcaggccg gagtggtatg cggtgccgca ggcgacgatg
       61 aagaccttgt cgatctcgcg cagctcctgg tcggagaggc gctgctcgtc gagcacgatg
      121 cggccgtcga cgaaatgccc cagcagggtg tcggagacgg cggcgggctg ctcggcgatc
      181 tccttgagca tgaagtactc gtagccaccc ttctcggcgg ccgacaggtc ccagtcgatc
      241 cgaaactcgc ggtagtcagt ggaaggatcg gcgtcgtttc cttcgaaatc ggtgatccgg
      301 tagccgtcgg cgttgaccac cacggcctgg tcctggccga gctcgacggc gttgcgggtg
      361 tgctcgatga atgccgccac atcggagccg acgaacatct cgccgtcgcc gacaccgagc
      421 accagcggcg tcgaccggcg ggcggcgatg atcgtgccgg gctcgtcggc gttggcgaac
      481 accaacgtga agtggccctc cagccgcggc agcacggcga acaccgactg cgtgaaatcg
      541 cttgccgtgt cacctttgcg gtattcgcgg gcgaccaggt gcacggcgac ttcggtgtcg
      601 gtgtcgctcg cgaactccac gccgtcggct tccaactcct ggcgcagcac ggtgtagttc
      661 tcgatgatgc cgttgtggac gacggcgatc ttgcccgacg catcgcggtg cgggtgggcg
      721 ttgcggtcgg tcggcctgcc gtgggtggcc cagcgggtat ggccgaggcc ggtgctaccc
      781 gcgaggctcg cggaatccgt ctcggccagc gccgcctcga ggttggccag gcgaccggcg
      841 cgccgccgga ccgtcaggcc gcctgccccg tcaagcaggg cgacgcccga agagtcgtag
      901 ccgcggtact ccattcggcg tagcgcgtcg acgacgactc cgcgagcgtc ccgcgtgcct
      961 acgtagccga cgattccaca cataggtatc cagcgtagac gactacccag ctaactgcat
     1021 aggcaccaca acccccgctg tgtagtgcgc tttgtaccta agcggtgact aagcgcaaac
     1081 ctggacgaat cccagggggc cgctgacgca cggcggcggc ggtggtccgt aattgttcca
     1141 cccgccatag tcgtaacccc caccccacgc cggaggcggt ggtggcggcg gaccgggccg
     1201 ccacgggccc ggcccaggcc cccagcctgg accccattcc gcgaagccgg cttgggtggc
     1261 cggcgacggc gccgcgttgg cggtggccgt tcccattccg agagcggcga cggccattgc
     1321 tgccgctacg ccgactcccg ttgcgaatcc aatcagtttc ttgttcatcc tcgatacccc
     1381 aaagtcgtta tcggacatcg ctcctcgtat cccgacccga cttcgacgct acctcggact
     1441 acatgtgaag ggcctgtgcg ggggttatgt atctcgacgc gccgggctcg gcgacgcgcc
     1501 aacgcagttt cacgacgacg accctaacgc cgcagttacc ggtgggttac ggtcgtcaag
     1561 tggccgccac caagaagctt ttcgccgctt tgagacgccg cggtccgcac cgcgtgctgc
     1621 gtggcgatct gtcgttcgcc ggtctacccg gcgtggtcta caccccggag tcgggctaca
     1681 accttcccgg tatcgccttc ggccacgaat ggctgaccgg tgtggagcac tatgagggca
     1741 cgctcgagca cctcgcctcg tggggcatcg tcgcggcggc acccgacacg gagaagggca
     1801 tcgcgccgtc gccgctgaac ctggcgttcg acctcggcac cacgctggac atcatcgccg
     1861 gcgtgcggtt gggcgagggg aagatcagcg tgcacccaaa caagctgggc ctcgtcggcc
     1921 acggtttcgg cgcgtcgacg gcggtgttcg ccgccgccgg tatgcccgcg aagcccaagg
     1981 ccgtggtggc ggcctttccg accgtcacca cgccgcgcgc cgaggaggcc gccgcgacgc
     2041 tcaaggtgcc cggcctgata ctcaccgatc cgggtgatcc gatgtcgctg cgatccaacg
     2101 cggtcgagct ggcgcgggtc tgggacgcgg cgacgctgag ggcgatgccg aaagccaagg
     2161 caggcgggtt gatcgagggg cgaaggctgg ctcgagttgt cggtctgccc ggtgcagacc
     2221 gcggcaccca gaagatggtg cgggcggtga tgaccggcta tctgttgcac gtacttgccg
     2281 gtgacaagac ctaccgcgac ttcgccgacc ccgaggcggc cctccccaag gcgacaacca
     2341 tggatccgtt cgccgacgac cccgttgacc tggagaacaa ggtcgtcgcg ctgttgaagc
     2401 cctgagtggc cgaccaaccg gtcgggcgta tgacccgtct gggtctacag tgccctccat
     2461 gcggacggga atctttctga actacgcagg cggcttcctg gaagccgtcg acgaggtcgt
     2521 cgagctggag aaggtcggcg tggacatcgc gctggtggcc gaggcgtact cgttcgacgc
     2581 gatcagccag ctgggtttcc ttgcggcgaa gacatcgcgg atcgagctgg gctccggagt
     2641 cgtcccgatc tacgtccgca ccccgtcgct gctggcgatg acggccgcgg gtcttgacta
     2701 cgtgtccgac ggccggttcc ggttgggcat cggcacgtca gggccgcagg tgatggaggg
     2761 ttttcacggc gttccgttcg acgccccgct cggccgcacc cgcgaggtcg tcgagatctg
     2821 caggcaggtg tggcgtcgcg agaacgtcga gttcgacggc aagcactacc agataccact
     2881 tccccaccac cggggcaccg ggttgggcaa gccgctacac ctgatcaatc accctgtgcg
     2941 ggaacgtatt ccaatcacga tcgccgcgct cggtcccaag aacgtcgagt tgaccgcgga
     3001 gatcgccgag ggctggcagc cggtgttctt ctatccggag aaggcagacg acgcctgggg
     3061 tgactcgttg cgcgcaggta tggccaaacg tgacccgcag ctcggaccgc tcgacatcat
     3121 ggtcagcgcc accttggcca tcggtgacga cgtcgaggac cggctggcgt gggcgaaacc
     3181 gcaactggcg ctgtacatcg gcggcatggg cgctcgcggc aagaacttct atcacaacct
     3241 ggcgacgcgg tacggattcg gcgaggtggc cgatcgcatc caggacctct acctgtccgg
     3301 ccgcaagacc gaggcgatcg atgccgtgcc cgacgatctg gtacgacatg tatcgctcat
     3361 cgggccaaag ggtttcgtca aggaacgtct cgccgcctat gcggaagccg gcgtgacgac
     3421 gctgctggtg catccgctga ccggcgaccg tcgcgagtcg gtgcgcttcg tcgaggagct
     3481 ccgggagctg ctcccctgat tagtcgcctc gtcacggccc ggcctgcggc agttcgtcgg
     3541 cggcgatctc acacggcgtt tcggccccga ccggcgcggc atccggcggc gattcgacga
     3601 gcggttccgg tggcggcggc atcggcgcgg gcggcggcgt cgggaccggg tcgggttgtg
     3661 gcgcttcgtc gacgggctcg tcgtgcccgc acgcgtcgac gggttcggcc gtcggctcac
     3721 cttcttctgc gagttcggcc gtctccacgt cgggcccgcc ttctgtttgc tcgtcctcct
     3781 cgtcttttgc gggctcattc tcatcgggcg ccggcttgtc ctcgtccacg gcatcgttgg
     3841 tcacttccga cggatcgggc agcgcgtcgt ggggttggtc gagcaggccg ctgatagcgt
     3901 cggcgagctg cgagccaatg cctgcgagtc cgcctgcggc gccgggcaat ccgccaccga
     3961 gctcaccgag tggcggcatg gtgggcatcg caggggtcgc tgacgctggg ccggtcgcag
     4021 gcgccgcggc cgcgggaagg gccgccgcgg gcagatcggg cgcggcggcc acaggcgcgg
     4081 cgggcacggg cgctacgggg gccgacgacg gacccaccgc cggcgccgcc gccggagcag
     4141 ggctggacca gacagccggc tccgtgcccg acaggtgggc cggtgatgca ctgctcggcg
     4201 gaggcggcac cgacgtcggc ccaagatcac ccggtacctc gaacggcgaa gtcggatcac
     4261 cagtcagggc gctggttgca gcgtcgaacg ccgcggagac ggcggccatc gcgcgctgca
     4321 tcgccgccaa ccagtccgct cgaatgtcgt tgtccacgaa cggcttcacc tgctgatcga
     4381 tcagctcgct cgccgccgcg cggtcccccg ctccagtggt caccgttcgc gtggcggtca
     4441 gccactcggc acgctgcgcc tgacggcggt cgtcgatcgt cacggcgcca gcgaccttgt
     4501 cg
//
LOCUS       DASYLX010001002         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_724585,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001002 DASYLX010000000
VERSION     DASYLX010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724585"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VGP27_23530"
     CDS             <1..1035
                     /locus_tag="VGP27_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV7854301.1"
                     /translation="CFHNLRDLDVDLPLGVYTAVTGVSGSGKSTLVVKVLGDVVKRHL
                     GRAPGQTESDTDAEDSDVEIVDLDHDASIGVTAENVDVIDRLVAVDQRPIGRTPRSTL
                     ATYTGLFDAVRREFAATPAARRHGWTAGRFSFNVAEGRCKTCQGEGFVSVELLFLPGT
                     YATCPTCHGARYNEATLRVRYRDRTIADVLAMTVDEASEFLADIPGAARSLTTLQEVG
                     LGYLRLGQPATELSGGEAQRIKLASELQRPRRGHTLYVLDEPTTGLHPADVDALDAQL
                     HRLVESGNTVVVAEHDMRMVAGSDWVIDLGPAAGNDGGRVVAAGTPQKVSQAKGSRTA
                     PYLAARLITR"
     gene            complement(1025..1534)
                     /locus_tag="VGP27_23535"
     CDS             complement(1025..1534)
                     /locus_tag="VGP27_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7854302.1"
                     /translation="MSLWDPTKVPPYPPPRYTRDEPEISAWLRRGTDPPDYDSSGLVQ
                     YHYLANQKQTDGDYGLYRVEIAPHGGGPGPHFHRAMSEAFFVLSGTVKLYDGTDWVDG
                     HPNDFLYVPPGGIHGFRNEADAPTSILMLFAPGAPREHYFEGLVRLGDMTDDERREWF
                     IRNDNFFIE"
     gene            1600..2262
                     /locus_tag="VGP27_23540"
     CDS             1600..2262
                     /locus_tag="VGP27_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005177071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV7854303.1"
                     /translation="MLLDAQDKAAQLFAEIERTGMVRAGVREKELSDEIRDLAADMFG
                     VNRHWHKRIVRAGANTLQPYRQNPPDRLIADDDIVFCDFGPIFERWEADFGRTFVLGD
                     DAGKHALCDDLPRVWAKGREYFDAHRDVTGAELFDHVVRLSREAGWEFGGPHAGHLVG
                     EFPHETIDGDSIESYIAPGSDHPMRRTDRTGCVCHWILEVHLIDPQRRFGGFYEELLD
                     LA"
     gene            complement(2235..>2576)
                     /locus_tag="VGP27_23545"
     CDS             complement(2235..>2576)
                     /locus_tag="VGP27_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEV7854304.1"
                     /translation="TNTIAAAAHPGGSRTELTRNLPWAVGAVSGLLAPLFQGADMGAL
                     PTLRAATDPGVIGGQYFGPDGFGEQRGYPKVVASSQDSHDIDAQRRLWTVSEQLTGVE
                     FPVYAKSSSSS"
BASE COUNT          435 a          836 c          890 g          415 t
ORIGIN      
        1 tgctttcaca acctgcgcga cctggacgtc gacctgccgc tgggcgtcta caccgcggtc
       61 accggtgtgt ccggctcggg gaagtcgacg ctcgtcgtca aggtgctcgg tgacgtggtg
      121 aaacgccatc tgggccgcgc gccgggtcag accgaaagcg acaccgacgc agaggattcc
      181 gacgtcgaga tcgtcgacct cgatcacgac gccagcatcg gagtgacggc ggagaacgtg
      241 gatgtgatcg accgactggt cgccgttgac cagcggccga tcgggcggac gccgcgttcg
      301 acgttggcca cctatacggg attgttcgac gccgttcggc gggaattcgc cgcgacgcct
      361 gcggcgcgcc gacacggctg gacggccggc cggttctcgt tcaacgtcgc cgagggtcgc
      421 tgcaagacct gtcagggtga aggcttcgta tcggtcgaat tgcttttcct gcccggcaca
      481 tacgcaacct gcccgacgtg ccacggcgcg cgctacaacg aggccacgct gagggtgcgg
      541 tatcgcgacc gcaccatcgc cgacgtactg gctatgacag tcgacgaggc ttcggagttc
      601 ctcgccgata tccctggcgc ggcgcgcagc ctgacgacgc tgcaggaggt ggggcttggc
      661 tacctgcggc tcgggcagcc cgcgaccgaa ttgtccggcg gcgaggcgca gcggatcaag
      721 ttggcatcgg aactgcaacg accccgccgc ggccacacgc tctacgtgct cgacgaaccc
      781 accaccggcc tgcatccggc cgatgtggac gcactcgatg cgcagctgca tcgcctcgtc
      841 gagtcgggca acaccgtagt ggtcgccgaa cacgacatgc ggatggtcgc cgggtcggac
      901 tgggtgatcg atctcgggcc tgccgcgggc aacgacggcg gccgggtggt cgcggcggga
      961 acaccgcaga aagtgagtca ggccaagggc agccgcaccg cgccgtacct ggccgcgagg
     1021 ctgatcactc gatgaagaag ttgtcgttgc ggatgaacca ttcccggcgc tcgtcgtcgg
     1081 tcatgtcgcc gagccggacc aggccttcga agtagtgctc ccgcggcgcg ccgggagcaa
     1141 acagcatcag gatcgacgtc ggcgcgtcgg cttcgttgcg gaagccgtgg atgccgcccg
     1201 gcgggacata gaggaagtcg ttgggatgcc cgtcgaccca gtccgtgccg tcatacagct
     1261 tgactgttcc cgacaacacg aagaacgcct cggacatcgc gcggtgaaag tgcgggccgg
     1321 gcccgccgcc gtgcggggcg atctcgacgc ggtacagccc gtagtcaccg tcggtctgct
     1381 tctggttggc cagatagtgg tactgcacaa gaccggacga gtcgtagtcc ggcggatccg
     1441 tgccgcgccg cagccaggca ctgatctccg gctcgtctct ggtgtaccgc ggcggcgggt
     1501 agggcggcac cttcgtcgga tcccacagtg acatcaacgc tccattgcag tcgtaacatc
     1561 ggcggagtga cggtcgcgaa cgaagatctc cgcaccgaga tgctcctcga tgcccaggat
     1621 aaggcggcgc agttgttcgc cgagatcgag cgcaccggca tggtccgcgc cggcgtccgt
     1681 gagaaggagt tgtccgacga gatccgcgac ctggcggccg acatgttcgg cgtgaatcgg
     1741 cactggcaca agcgaatcgt gcgggcgggc gcgaacaccc tgcagccgta ccggcagaat
     1801 ccaccggacc ggctcatcgc cgacgacgac atcgtgttct gcgacttcgg cccgatcttc
     1861 gagcggtggg aggccgactt cggccgcacg ttcgtgctgg gcgacgacgc gggcaagcac
     1921 gcactgtgcg acgacctgcc gcgggtctgg gcgaagggcc gggagtactt cgacgcgcac
     1981 agggacgtga ccggcgccga gctgttcgac catgtcgtgc ggctcagccg tgaggcgggc
     2041 tgggagttcg gcggacctca cgccggccat ttggtcggcg agttcccaca cgagacgatc
     2101 gacggcgact cgatcgagtc gtatatcgcg ccggggtcag accacccgat gcggcgcacc
     2161 gaccgcaccg ggtgtgtctg ccactggatc ctggaagtcc acctgatcga tcctcaacgg
     2221 cggttcggtg gcttctacga agagctgctc gacttggcgt agaccgggaa ctcgacaccg
     2281 gtgagctgtt cggacaccgt ccacagccgg cgctgcgcgt cgatgtcatg ggaatcctgg
     2341 ctggacgcaa caactttggg gtagccgcgc tgttcaccga agccgtcggg gccgaagtac
     2401 tgcccgccga tcacgcctgg atccgttgcc gcgcgcaatg tcggcagcgc acccatgtcc
     2461 gcgccctgga acagcggcgc cagcaggccg gaaaccgcac cgacggccca gggtaggttg
     2521 cgcgtcagct ctgtgcgcga gccgcccggg tgtgctgcgg cggcaatggt attcgt
//
LOCUS       DASYLX010001003         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1929147,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001003 DASYLX010000000
VERSION     DASYLX010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929147"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..394
                     /locus_tag="VGP27_23550"
     CDS             263..394
                     /locus_tag="VGP27_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854305.1"
                     /translation="MWIDDTSADVIKVDFEALYHGDVLVEGDTSEQLDDDQQLPYAV"
     gene            complement(488..1486)
                     /locus_tag="VGP27_23555"
     CDS             complement(488..1486)
                     /locus_tag="VGP27_23555"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEV7854306.1"
                     /translation="MSIRTKFTETFGVEHPVVQGGMQWVGRAELVAAVANAGALGFIT
                     ALTQPTPADLAKEIAKCRDLTDKPFGVNLTILPTINPPPYDEYRQVIVDAGIKIVETA
                     GSNPAPHLPMFHENGIKVLHKCTSVRHAVKAQGLGVDGISIDGFECAGHPGEDDIPGL
                     VLIPAAAAKIDIPMIASGGFADGRGLVAALALGADGINMGSRFMCTAESAIHQKVKEA
                     IVAGTELDTEMIFRPLRNTARVASNAVSREVVEILNNGGQFEDVKDLVAGSRGVKVYE
                     IGDLDAGIWSVGTAMGLIDDIPTCDELISRIVADAEELIIERLADMVDTTAKENVA"
     gene            complement(1539..2291)
                     /locus_tag="VGP27_23560"
     CDS             complement(1539..2291)
                     /locus_tag="VGP27_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEV7854307.1"
                     /translation="MEIKDAVAVVTGGASGLGLATTKRLLDRGASVVVVDLRGEEAVA
                     ELGDRAQFVETNVTDPDAVTAALDAAEKMGPLRINVNCAGIGNAIKTLGKDGPFPLDG
                     FKKVIEVNLIGTFNVLRLAAERIAKTEPINGERGVIVNTASVAAFEGQIGQAAYSASK
                     GGVVGMTLPIARDLSRDLIRVVTIAPGLFKTPLLGSLPEEAQASLGKQVPHPARLGDP
                     DEYGALAVHIVENPMLNGEVIRLDGAIRMAPR"
BASE COUNT          379 a          798 c          713 g          469 t
ORIGIN      
        1 gtactcggtc gacgcgaagg tgctcgggag ggtgcgttgg tcgcccagaa tcgcggccgg
       61 gttgctcgct ctggcgttcg ccgtggcgat cctgacctgg acactgctga atggcacaaa
      121 tccgattcat ttcagctcac cggcataatc ccaggtcagc tactcgtcag taaccgccga
      181 ccctaccggg cggtaggaat tgccacgatt ttccgaatcg tgactcaaag aatccttaaa
      241 agtcacccat aggctcagtg tcatgtggat cgacgacacc agtgctgatg tcatcaaggt
      301 tgatttcgag gctctctacc acggcgatgt tctcgtcgag ggcgacacct cagagcagct
      361 tgacgacgac cagcagctgc cctacgcagt ctgagtcacc gcgctcgcac cgagcgcgct
      421 gacccgtcag acagtttgcc cctcgcgggg ccttcgttga ttctttgttg cttccgaaac
      481 cgctggctta cgcgacgttc tctttcgccg tcgtatccac catgtcggcc agtcgttcga
      541 tgatcagctc ctcggcatct gccacgatgc gtgaaatcag ctcatcgcac gtcggaatgt
      601 cgtcgatcaa tcccatcgcc gtgccgaccg accagatacc ggcgtccaga tcgccgatct
      661 catagacctt gacgccgcgg gagcccgcga cgaggtcttt gacgtcctcg aactggccgc
      721 cgttgttgag aatctccacc acctcgcgcg acaccgcgtt gctcgcgacc cgcgcggtgt
      781 tgcgcagcgg ccggaagatc atctcggtgt cgagttcggt gcccgccacg atcgcttcct
      841 tgactttttg gtggatggcc gactcggcgg tgcacatgaa ccgcgagccc atgttgatgc
      901 cgtcggcgcc aagggccagc gcagcgacca gtccgcggcc gtccgcgaag ccgcccgagg
      961 cgatcatcgg gatgtcgatc ttggcggccg ccgcggggat caacaccaag cctgggatgt
     1021 cgtcctcgcc cggatggcct gcgcattcga aaccgtcaat gctgatgccg tcgacaccga
     1081 ggccctgcgc cttcaccgca tggcgcaccg aggtgcactt gtgcagcacc ttgatcccgt
     1141 tctcatgaaa catcggtaga tgcggcgccg ggttcgagcc tgcggtctcg acgatcttga
     1201 tgccggcgtc gacgatcacc tgccggtact cgtcataggg cggcgggttg atcgtcggca
     1261 ggatggtcag gttcacgccg aacggcttgt ccgtgagatc gcggcacttg gcgatctcct
     1321 tcgccagatc cgcaggagtc ggctgcgtca acgcggtgat gaaacccagc gcccccgcat
     1381 tcgctactgc cgcaacgagt tccgcgcggc ccacccactg cataccaccc tgcacaacag
     1441 ggtgctcgac cccgaacgtc tcggtgaact tcgtcctgat gctcatcgga attttctctc
     1501 ccctgcactt cctctgtggg cccacttcgc gcaagcgctc atcggggcgc catcctgatc
     1561 gcgccgtcga ggcggatgac ctccccgttc agcatcgggt tctccacgat gtgcacggcc
     1621 agcgcgccgt actcgtcggg gtcgccgagg cgcgcggggt gcggcacctg cttgccgagc
     1681 gaggcctgcg cctcctcggg cagcgagccc agcagcgggg tcttgaacag acccggcgca
     1741 atcgtcacca cgcggatcag gtcgcgcgac agatcgcggg cgatcggcaa cgtcatgccg
     1801 acgacgccac ccttggacgc cgaatacgcc gcctgcccga tctggccttc gaacgccgcc
     1861 accgacgcgg tgttgacgat gacgccgcgc tcgccgttga tcggttcggt cttggcgatc
     1921 cgctccgcgg ccaatcgcag cacgttgaag gtgccgatca ggttgacctc gatgaccttc
     1981 ttgaacccgt ctagcgggaa cgggccgtcc ttgccaagcg tcttgatcgc gttgccgatg
     2041 cccgcgcagt tcacgttgat gcgcagcggg cccatcttct cggcggcgtc gagggctgcg
     2101 gtgacggcgt cgggatcggt gacgttggtc tcgacgaact gggcgcgatc gccgagttcg
     2161 gccaccgcct cctcacctct gaggtcgacg acgaccaccg aggcacccct gtcaagcagc
     2221 cgcttggtgg tcgccaggcc gagacctgag gctcctccgg tgacgacggc tacggcgtct
     2281 ttgatctcca cttagtgcgt tccttccttt gtgccgtgct tctcctcggg ccgttgcggc
     2341 ccgcatcgtc gcgtggcgt
//
LOCUS       DASYLX010001004         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1380707,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001004 DASYLX010000000
VERSION     DASYLX010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380707"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGP27_23565"
     CDS             complement(<1..288)
                     /locus_tag="VGP27_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEV7854308.1"
                     /translation="MSEAESHPPGRVEVHRVRKMSGRDPEETHRVATPLELLFDLTFV
                     IGFGISASEFAHALAEDHVGAGLAGFAFATFAICWAWINFSWFASAYDTDDW"
     gene            452..958
                     /locus_tag="VGP27_23570"
     CDS             452..958
                     /locus_tag="VGP27_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV7854309.1"
                     /translation="MSEQFTITLLGDFEVYRDGQLLDLPPSCQRLIALAVLKRRAVPR
                     SWLCRTLWPTTRPDRATARLRTTLWRLRPMGAEALLTITPQSIGISPDVSVDWYDSVD
                     LIGELLGHCGPSRPDRDLAAELLPLLGGGALLDGWTDDWNAYARDTYRELRLDALETL
                     MGACAQNG"
     gene            1170..>1500
                     /locus_tag="VGP27_23575"
     CDS             1170..>1500
                     /locus_tag="VGP27_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV7854310.1"
                     /translation="MDGLTDDVVAFISEGTRTGKLGYTASDGRPLVAPVWFVVDDGEL
                     AFSTGKNTAKGRALARDSRSVICVDDDRPPYSFVQVQGTVSISEDPQVLVDIATRTGA
                     RYMGADRA"
BASE COUNT          256 a          489 c          500 g          255 t
ORIGIN      
        1 ccagtcgtcg gtgtcgtagg ccgacgcgaa ccagctgaaa ttgatccacg cccagcagat
       61 ggcgaaggtc gcgaatgcga acccggccag ccccgcgccc acgtggtcct cggcgagcgc
      121 atgcgcgaac tccgaggccg atatgccgaa gccgatcacg aacgtgagat cgaaaagcaa
      181 ctccagcggc gtggcgaccc ggtgcgtctc ttcggggtcg cggccggaca tcttgcggac
      241 gcggtgcacc tcgacccttc cgggtggatg tgactcggcc tcactcatga cctgcagagt
      301 ttgcccagcc ggcgcggcac tgtccaggca ctcgcccgtg ggatctgcgc agagggtgcg
      361 cagaaagagg tagtcgacta ctcacgccgt cgcgtcccgc gctttgcatc atcgacctca
      421 gagcgtcgga tgagatggaa ggcaggctgc gatgtcggaa cagttcacga tcacgctgct
      481 gggtgatttc gaggtctatc gcgacggaca gctgctcgac ctgccgccgt cgtgtcagcg
      541 actgattgcg ctcgccgttc tgaaacgccg ggcggtgccc cgcagttggc tgtgccggac
      601 gctgtggccc accacgcggc ccgatcgtgc gacggccagg ttgcgcacga cgttgtggcg
      661 gctgcgtccc atgggcgccg aagccctgct gaccatcaca ccgcaatcga tcggcatctc
      721 gcccgacgtg tcggtggatt ggtatgactc ggtggacctg atcggcgaac tgctcggtca
      781 ttgcggacct tcgcggccgg acagggacct cgccgccgag ctgctgcccc tgctgggagg
      841 gggtgccctg ctcgacgggt ggaccgacga ctggaacgca tacgcccgcg acacgtatcg
      901 cgagctgcgg ctcgacgccc tcgaaacact catgggggca tgcgcccaga acgggtagcg
      961 cactactcgt gcgtgtcgct gtgaacgatt cctacgctta cgtcgtcaaa tagatacaca
     1021 cagatgggaa atgaagaagg ccatgactcc cctactcgtg atctgcctga gcctcgcggc
     1081 gacgctcgtc gcgggcggcg tgcacgagtt gcagtcgcgg ctcgagcgtt gggactacga
     1141 gcggcacgca gaggactagc ctcggctgag tggatggact caccgacgac gtcgtcgctt
     1201 tcatctccga gggcacccgg accggaaagc tcggttacac cgcatccgac ggccgcccgc
     1261 tggttgcgcc cgtctggttc gtcgtggacg acggcgagct ggccttcagc accggcaaga
     1321 acaccgccaa aggccgtgca ctggctcgtg actcgcggtc ggtgatctgc gtggacgacg
     1381 accggccgcc gtattcgttc gtgcaggtgc agggcaccgt gtcgatcagt gaggacccgc
     1441 aggttctggt cgacatcgcc acccgcaccg gcgctcgcta catgggggcc gaccgggccg
//
LOCUS       DASYLX010001005         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1052381,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001005 DASYLX010000000
VERSION     DASYLX010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052381"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGP27_23580"
     CDS             complement(<1..362)
                     /locus_tag="VGP27_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme A pyrophosphatase"
                     /protein_id="HEV7854311.1"
                     /translation="MTSESERSPLAPDAAPAWLKPLVDNAHTVKRAYRRRVPAELLAM
                     VTAANAKAALTGAGRDAAVLVLFSGPQDSLNGGLPDDADLLVTVRASTLRHHAGQAAF
                     PGGAADPGDDGPIHTALR"
     gene            complement(359..1015)
                     /locus_tag="VGP27_23585"
     CDS             complement(359..1015)
                     /locus_tag="VGP27_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV7854312.1"
                     /translation="MSTSTRWTVAVLAVVVALGVGLWVQLDRSGPTGGPGQAPGQAPA
                     REQRDADTSEALAEPRARAHLPPCPADGPGEGPPKLRGVTVECAGDGADVNVAKALAG
                     RAVVLNLWAYWCGPCADELPAMAEYQRRMGSAVTVVTVHQDENETAGLLRLAELGVRL
                     PTLQDGGRLIAAALQVPNVMPATVVLRPDGSVAEILPRSFASADDIAAAVGPQIGVPG
                     "
     gene            complement(1033..1713)
                     /gene="nth"
                     /locus_tag="VGP27_23590"
     CDS             complement(1033..1713)
                     /gene="nth"
                     /locus_tag="VGP27_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV7854313.1"
                     /translation="MNRELAQAFPHVYCELDFTNPLELTVATILSAQSTDKGVNLTTP
                     ALFKKYRTAKDYAQADRTELEEMVRPTGFYRNKANSLIRLGQELVERFDGEVPATMAE
                     LVTLPGVGRKTANVILGNAFDIPGITVDTHFGRLVRRWRWTEFEDPVKVEHAVGELIE
                     RREWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGEGPTDPLVAAPLVKGPETE
                     HLLALAGL"
     gene            1964..2266
                     /locus_tag="VGP27_23595"
     CDS             1964..2266
                     /locus_tag="VGP27_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854314.1"
                     /translation="MLATFGLERLESGLDHDTVSAADVAEFLEQAQADDVRTLALDGM
                     PTALDGMHRRLHERFADAEVARPLSIEVDWPNLPTPVYAHNSENPEFQPSRHANRV"
     gene            2368..>2864
                     /locus_tag="VGP27_23600"
     CDS             2368..>2864
                     /locus_tag="VGP27_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7854315.1"
                     /translation="MDEILARAGIFQGVEPTAVSALTKQLQPVDFPRGHTVFAEGEPG
                     DRLFIIISGKVKIGRRSPDGRENLLTIMGPSDMFGELSIFDPGPRTSSATTITEVRAV
                     SMDRDALRAWIADRPEIAEQLLRVLARRLRRTNNNLADLIFTDVPGRVAKQLLQLAQR
                     FGTQEG"
BASE COUNT          463 a         1005 c          919 g          477 t
ORIGIN      
        1 tcacgcagcg cggtgtgtat cggaccgtcg tcgccgggat cggccgctcc gccgggaaac
       61 gcggcctggc cggcgtggtg gcgcaatgtc gaagcccgga cggtcaccag caggtcggca
      121 tcgtcgggca gtccgccgtt cagggagtcc tgtggacccg agaagagcac cagcaccgcg
      181 gcgtcgcgac ccgcgccggt cagcgcggcc ttcgcgttgg ccgcggtcac catcgccagc
      241 agttccgcgg gcacccgtcg ccgatacgcc cgtttcaccg tgtgagcgtt gtctaccagc
      301 ggcttcagcc aggccggagc ggcgtcggga gccagcggac tccgttcgct ctccgaggtc
      361 accccggcac tcctatctgt ggaccgaccg cagcggcgat gtcgtcggca ctggcgaacg
      421 accgcggcaa gatctcggca acgctaccgt ccgggcgcag caccacggtt gcgggcatca
      481 cattggggac ctgcagcgct gcggcgatca aacgcccgcc gtcctgcagc gtcggcagcc
      541 gaacacccaa ctcggcaagc cgcagcagcc ccgcggtctc gttctcgtcc tggtgcaccg
      601 tcacgactgt caccgccgag cccatccggc gctgatactc ggccatggcg ggcagttcgt
      661 cggcgcacgg cccacaccaa tacgcccaca ggttgagcac caccgcgcgg ccggcaaggg
      721 ccttcgcgac gttgacgtcg gcgccgtcac cggcacactc gaccgtgact ccccgaagtt
      781 tcggcgggcc ctcaccgggg ccgtcggccg ggcatggcgg caggtgtgcc cgcgcccgcg
      841 gctcggcgag cgcctccgag gtgtcggcgt cgcgctgctc cctggcgggc gcttggcccg
      901 gtgcttggcc cggcccgccc gtcggtccac tccggtccag ctggacccac agcccgactc
      961 cgagcgcgac gacgacggcc agcaccgcca cggtccatcg ggtcgacgtg ctcatgacgc
     1021 gggcgaggtc ggctacagcc cggccagcgc cagcaggtgt tcggtctccg gccccttcac
     1081 cagcggcgca gcaacaagcg gatccgtcgg tccttccccg aacgacgggc agtccttggc
     1141 gagtacgcac acgccgcacg ccggcttgcg ggcatggcag acgcggcggc cgtgaaagat
     1201 cacccggtgg ctgagcaagg tccactcgcg gcgctcgatc agctcaccga ccgcgtgctc
     1261 gaccttcacc gggtcctcga actcggtcca ccgccaccgc cggaccagcc tgccgaagtg
     1321 tgtgtcgacc gtgatgccgg gaatgtcgaa tgcgttgccg agaatcacgt tggcggtctt
     1381 ccggccgacg ccgggcagtg tcacgagctc cgccatggtc gcaggcacct cgccgtcgaa
     1441 ccgctcgacg agttcctggc ccagccggat gagcgagttc gccttgtttc ggtagaagcc
     1501 cgtcggccgc accatttcct cgagctcggt gcggtccgcc tgcgcgtagt ccttcgcggt
     1561 ccggtacttc ttgaacaagg caggggtggt caggttgacg cccttgtcgg tgctctgcgc
     1621 cgaaaggatg gtcgctaccg tgagctccag cgggttggtg aagtccagct cgcagtacac
     1681 atgcggaaac gcctgggcca gttcgcgatt catccgccgc gcccgtcgga ccaagccgat
     1741 atgcgtctcc gaatcccatt tgcgcgcagc ggcctgcccg cgcggcttgg cctgcgccgc
     1801 tttctcggcc gaaggcccac caacagtcac gctgccagcg tactgagcgc gccgacaatc
     1861 cgaggtgcgg accggtgaca attcgtgatc cgaccgagat gttcggcgtg tttactacct
     1921 ccttgtgtcg tcgttgctcg tggtattaat cccggggttg ctgatgctcg cgaccttcgg
     1981 gctggagcgc ctcgaatctg ggctcgacca cgacaccgtt tcggccgccg atgtcgccga
     2041 atttctcgag caggcgcagg ccgacgacgt tcgcactctg gcgctggacg gcatgccgac
     2101 ggcgctggac ggcatgcatc gccgtttaca cgaacgcttc gccgatgcag aggttgccag
     2161 gccacttagc atcgaagtcg actggccgaa tttgccaacg ccggtctatg cacacaacag
     2221 cgaaaatcct gaatttcagc cgtctcggca cgccaatcgt gtgtagcgtg actaggcacg
     2281 aaatggccag cctctagact gatctcacaa accgtttaga ggtcggcttg cgcatgccat
     2341 atcactcaac agcttaagag gggcaacgtg gacgagatcc tggccagggc cggaatcttc
     2401 cagggagtcg agccgaccgc ggtatccgca ctgacgaagc aactgcagcc cgtcgacttc
     2461 ccccgcggac acaccgtgtt cgctgaaggt gagcctggcg atcggctatt catcatcatc
     2521 tcgggcaaag tgaagattgg tcgccggtcc ccggacggac gcgagaatct gctcaccatc
     2581 atgggcccgt cggacatgtt cggtgaattg tcgatcttcg accccggacc gcggacgtcg
     2641 agtgcgacca ccatcaccga ggtgcgcgcg gtctcgatgg atcgcgacgc gctgcgcgcc
     2701 tggatcgccg atcgaccgga gatcgccgag cagctgctgc gggtgctcgc ccgccggctg
     2761 cgtcgcacga acaacaacct cgccgatctg atcttcaccg acgtccccgg ccgcgtcgcc
     2821 aagcagctgc tgcagctggc acagcgcttc ggcacgcaag aggg
//
LOCUS       DASYLX010001006         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1052445,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001006 DASYLX010000000
VERSION     DASYLX010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052445"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGP27_23605"
     CDS             <1..1033
                     /locus_tag="VGP27_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEV7854316.1"
                     /translation="DFDGTLTGGYTAHPKRDPDTGELHAVSYSMYRGNTVQYSVIGTD
                     GRARRTVDIEVTGSPMMHDMSLTEKHVVFYDLPVTFDTHQAAAMTVPRSLRLPARLLL
                     SALIGRVRIPDPVSARQPGGNASDRRFPYSWNPKYPARVGVMPRDGDNSDVRWFDVEP
                     CYVFHPMNAFDDGDTIVLDVVRHPKMFATEQLGPNEGPPTLDRWTVDLADGKVRESRV
                     DDRGQEFPRVDERLVGKRHRYGYAPTVGEGVSGNDVLLKHDFLGNGTVSRSFGADKAL
                     GEFVFHPSAPDAPEDDGVLMGYVYDRAADRSELAILDAHTLQDVASIKVPHRIPSGFH
                     GNWVPAPAA"
     gene            complement(1176..1415)
                     /locus_tag="VGP27_23610"
     CDS             complement(1176..1415)
                     /locus_tag="VGP27_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854317.1"
                     /translation="MAHSVRRLTAAAAMATLPMAGVVVFTPAISSACLPGETAGPAAC
                     APFCLPGKALDVQTGLCVPVPDPAPNGVNPPIKDY"
     gene            complement(1421..>1739)
                     /locus_tag="VGP27_23615"
     CDS             complement(1421..>1739)
                     /locus_tag="VGP27_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV7854318.1"
                     /translation="PEELLLASLSQCHLLWYLDLAVRAGVVVTDYADDPIGSMVVNGD
                     GSGQFERVVLRPTVTVAEPVHTAAARRLHADAHRMCFIARSVNFPVDCEPTEPRVADA
                     ALP"
BASE COUNT          294 a          569 c          595 g          281 t
ORIGIN      
        1 tgacttcgac ggcacgctga cgggtggtta caccgcgcac ccgaagcgcg accccgatac
       61 cggtgagctg catgctgttt cgtacagcat gtaccgcggc aacacggttc agtactcggt
      121 gatcggcacc gacgggcggg cgcggcgcac cgtcgacatc gaggtgacgg gcagcccgat
      181 gatgcacgac atgtcgctga cggagaagca cgtggtgttc tacgacctgc cggtcacctt
      241 cgacacacac caagcggccg ccatgacggt gccgcgaagc ctcaggcttc ccgcgcgact
      301 tctgttgtcc gccctgatcg gccgcgtccg catccccgat cccgtttccg ctcgacaacc
      361 gggtggcaac gcgtccgatc ggcgcttccc gtactcgtgg aacccgaaat atccggccag
      421 ggtcggggtg atgccgcgcg acggcgacaa ctcggacgtc cgctggttcg acgtcgagcc
      481 gtgctacgtc ttccacccga tgaacgcctt cgacgacggc gacacgatcg tgctcgatgt
      541 cgtgcggcac ccgaagatgt ttgcgaccga gcagctggga ccgaacgagg ggccgccgac
      601 gcttgatcgg tggacggtgg acctcgccga cggcaaggta cgcgaatcgc gggtcgacga
      661 ccgcggccag gagtttccgc gcgtcgacga gcgactggtc ggcaaacggc accgctacgg
      721 ctacgcgccg acggtcgggg agggcgtcag cggcaatgac gtgcttctca agcacgactt
      781 cctcggtaac ggcaccgtgt cacgctcgtt cggcgccgat aaggcactcg gcgagttcgt
      841 cttccatccg tccgcgcccg atgcgccgga ggacgacggt gtgctgatgg gctacgtgta
      901 cgaccgtgca gccgaccgca gcgaactggc catcctcgat gcgcacacgt tgcaggatgt
      961 cgcgagcatc aaggtgccgc accgaattcc gtcaggattc cacggcaatt gggtgccggc
     1021 gcctgcggcg taacgcacta ttacctgatg ggctccgtcg ccggtgctgt cgagcgcgcg
     1081 gctatgcgga cggtgcgtac gccagcggtg cgtgcggcga aatggcttgt ggctcagcag
     1141 gcctaaggca cacagttctg tgccctgcgc cgggcttagt agtctttgat cggcgggttg
     1201 acgccgttgg gtgccgggtc cggcaccgga acgcagaggc cggtctgtac gtcgagcgcc
     1261 ttgcccggca ggcagaacgg cgcgcaagcg gcggggcctg ccgtctcgcc gggcaggcac
     1321 gccgagctga tcgcaggcgt gaagacgacg acccccgcca tcggcagggt cgccatcgcc
     1381 gctgcggccg tcagacgtcg cactgagtga gccatggtgg ctagggtaac gccgcgtcgg
     1441 ctacccgagg ttctgttggt tcacagtcga cgggaaaatt caccgaccgg gcgatgaagc
     1501 acatccggtg ggcgtcggca tgcagtcgcc gtgccgccgc cgtgtgaacg ggctcggcga
     1561 ccgtgacggt gggccgcagg accacccgct cgaactgtcc acttccgtcg ccgttgacga
     1621 ccatcgagcc gatcggatcg tcggcgtaat cggtcaccac gacgccggcc cgcacggcca
     1681 ggtcgagata ccagagcaga tggcactgcg acagcgaggc gagcagaagt tcctcgggg
//
LOCUS       DASYLX010001007        37109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2258081,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001007 DASYLX010000000
VERSION     DASYLX010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 37109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258081"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGP27_23620"
     CDS             <1..361
                     /locus_tag="VGP27_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854319.1"
                     /translation="VAGVVLAIPLYCLGLMAAYLATRFGTVLGYGQSTGVYDHYFDTF
                     LNPTDVVLSFTQAIGMATIVMLIHTYYGYTASGGPAGVGEAVGRAVRASLVAVIVVLL
                     FVALGIYGQGGGLNISG"
     gene            366..1838
                     /locus_tag="VGP27_23625"
     CDS             366..1838
                     /locus_tag="VGP27_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854320.1"
                     /translation="MAAAARTKKRAGESRIHPGWWTLGLILVVVGLVVLDAVLFTGSY
                     RSSIPVTVMSDRSGLIMETGNDVKLRGVVVGRVEGISKGNPPVGLNLAIDSAQVKYIP
                     ANVEAEIKATTAFGNKFVDLVVPDDPSAKRLSAGEVLRARNVATEVNTVFENLVGVLQ
                     QVDTAKLNAVLSALAEGVRGQGERLGEAITAGNEVLMQLNPRSETIRQDWKAVKGFSD
                     TYSAAAQNIITVLDAASTTSTTITNHAKQLDSLLLNVIGLSNSGVNVLGPSKDNLIHA
                     VNVLDSTTSLLMKYNPELTCLFTGGKNVIDFGFENVAGGSTGKSVILDVALLFGDDQY
                     KYPDNLPINGAKGGPGGKPGCGSLPDVAQNWPQRQLITNTGWGTGVDIRPNPGIGFPG
                     YGNYFPFTRGVPQPPTMNRIGPPAPGPIPYPGAPPYGAQQYAPDGTPLYPGLPPAPPP
                     GQPKEPGPRAGEEPFVVPYPAQVQPNPPPPIPPGPVTPAP"
     gene            1883..2911
                     /locus_tag="VGP27_23630"
     CDS             1883..2911
                     /locus_tag="VGP27_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854321.1"
                     /translation="MMDSLRGTLVRIAIFLVVCTLGWFVTVAVFGQLRFDEQNKYNAV
                     FKNVSGLETDDFVRIAGVEVGKVKGIKVRDDATVDVEFAADKSVVLTEGSRVAIRYEN
                     LTGDRFVALEEGAGGTRKLNAGDTIPLERTEPALDLDALIGGFRPLFNALSPDQVNAL
                     TGQLIEAFQGQGATISSFLSQTASLTNTLADRDQLIGQVIVNLNTVLGSLGDQKEQFG
                     RAVGSLSQLVQGLAERKQDITNGVAYANAAAGSVADLLQQARPPLKKVVTEADRLAGN
                     IVADADYVNNLLDTLPDSYRILNRQGLYGDFFSFYLCDLVLKLNGKGGQPVYVKLAGQ
                     DTGRCTPR"
     gene            2908..4368
                     /locus_tag="VGP27_23635"
     CDS             2908..4368
                     /locus_tag="VGP27_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854322.1"
                     /translation="MKSFQERNPLIIGAIGLTLSVAVALGAVNYDKLPGLSVGKEYSA
                     YFAEAGGLLSGADVRVSGFAAGKVEDITLDDSRVLVTFKVDDDIRLGERSEAAVKTRS
                     ALGSKILEITPRGDGQLSGPIPLERTTPAYQLPDAIGDLTTAISGLDTNQLSDSLKML
                     SETFADTPPDVRAAVEGVGRFTQTLDERDAQLRSLLSNANKATAVLSERSDQIVTLVR
                     DTNALLAALRSESASLDQIFNNISAVSQQLKGFIAENRTQLKPALDKLNGVLAIVDNH
                     KEQVKLALQGLNRYALGLGESVSSGPFFKAYLVNLFPGQFVQPFIDAAFSDLGLDPNV
                     LLPTQRSDPQVGQPGTPALPIPYPRTGQGGDPKLTIPDAITGNPGDQGCGPPGVPLPG
                     PTGCYPYREPLPAPPPGGPPPGPPAAASPGQESTATPPLPIYAPAPGEQVPAGAQSRP
                     PGDPAVVGPGAPGAADVTPVPLGPPPGPPAPAGDGQ"
     gene            4365..5822
                     /locus_tag="VGP27_23640"
     CDS             4365..5822
                     /locus_tag="VGP27_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854323.1"
                     /translation="MNRLNSRTGKIGLAVALVVLLVLAGVTIFRSGAASKTTIVGYFD
                     NSNGIYTGDDVVILGVPVGSIDKIEPEPFRTKITFSVDDKYKVPADAKAVILSPALVT
                     ARAIQLTPTYKSGPKMQDGAVIPQERTAVPVEFDAFRQQLERLADTLQPTTPGGVSTL
                     GAFINTAADNLRGQGANIRDTLIKLSQAFSALGDNSKGLFGTVKNLAILVSALRDSSD
                     LLRQLNGNLASVTGLISNDPNEVGNAVKDLADVAGDVRSFVAENRETLGTTSDKLASV
                     TQNLADNIDEVKQLLHVAPNAFQNFLNIYQPAQGTLTGALALNNFANPIQFLCGAVQA
                     ASRLGAEQSAKLCVQYLAPIIKNRQYNFLPFGLNPFVGATARPNEVTYSEDWMRPDYI
                     PPAGAGPALPAEAPGSQAPSTPPSGGPLPPPGSVFTNFGEPADPNHGQPPFVPARPPD
                     PPPLNPGDAPAVPAPVSTDPGTGLTGMMVPPGGGQ"
     gene            5819..6958
                     /locus_tag="VGP27_23645"
     CDS             5819..6958
                     /locus_tag="VGP27_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HEV7854324.1"
                     /translation="MSGNHLRRILLGVVVVVVMAVPGCANWRGLNSITLPGTAGGEAG
                     AYTIKAQLPEVVNLQPNSRVRVGDVTVGNITKIERQDWHALVTMTIDGNVDLPANATA
                     KLGQTSLLGSLHIELAPPKEVPPQGKLKNGSLIPLSSAGTYPTTEETLSALSLLLNGG
                     GVGRIQEITKELSTAFSGRADDLRSLLSQLDVFVKYLNDQKDDILAAADSLNNLVGQI
                     ADQKPVVDKALKTVPQALAILKDERITLADALDQLGKFSALAADSVNQTKENLVKELK
                     DLGPVLESLANAGQDLTRSLDFYATFPFPKPTLNKWLRGDYANLTAVIDLTLSRLDAS
                     FLTGTRAEGELTELELQWGRTIGQMPSPYTARNPLVAPYRFDQGP"
     gene            6960..8435
                     /locus_tag="VGP27_23650"
     CDS             6960..8435
                     /locus_tag="VGP27_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854325.1"
                     /translation="MNITRRIWIQLGVVSLVSLAGVLLMFFPVLHLQERWFGVGRYTV
                     TVQLPQTGGLYERGNVTYRGYQVGQVKSVQLTNIGAEAVLSLNSDVKIPSDVNAEVHS
                     VSAIGEQYIDLLPRSADGPPLKDGSVIPVGQTSVPPNISSLLDATNKGLEAIPRDNLK
                     TVVDESYIAFGGLGPELARFVKGATALAIDARANIDDLTTLVDQSKPILDSQIDSSDS
                     IQAWASNLATVTSELRAQDGNLATVLQDAPGAVDEARALFDRLQPTLPIVLANLVTVG
                     DVAAVYRDNLEQLLVLLPQGAAAVQASGVANRNTKQDYNGAFLSFNLNLNLPPACTTG
                     FLPIQQQRAAAFEDYPPPPVGDLYCRVPQDSPLNVRGARNSPCVEKPGKRAPTAKMCE
                     SDENYVPLNDGYNWKGDPNATLSGQGIPQPRDPSAPGSGPVEGVPPAPAPAPPPVAFA
                     EYDPATGTYVGPDGQKHTQANLGEAKGKQTWQDMLTPPTGG"
     gene            8435..8938
                     /locus_tag="VGP27_23655"
     CDS             8435..8938
                     /locus_tag="VGP27_23655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854326.1"
                     /translation="MSESAPQTGRAQQAIGGGRVQIVELIGPVVRQAGCKDFVISCQH
                     PSPVAVKVPLAGDAGMMTSAFAHPGIFLQCPGLLGVDKHTVTSVGCAAGSQVFGLRVA
                     LTALAPPGSTTSSGGVNMYWQPAASDDSSTGTADAIAVAPPKALAKVAAAAADANIER
                     STIIGIV"
     gene            complement(8935..9549)
                     /locus_tag="VGP27_23660"
     CDS             complement(8935..9549)
                     /locus_tag="VGP27_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7854327.1"
                     /translation="MPSTPQDHDLAATAVVCVECQNGVLGPDSVLPALAADSAGLVAN
                     LRRLLDGARERGVRVVHATYEGSLGGDPTGTARLWRALGPATAAWAPGRVETQVLPEL
                     LAPTDMVLPRHHGLFPTLDTELLPVLKGLGVRTVVLTGVSLNLALPTTAGHTTQAGFR
                     LVVPRDAVGGTPMDYANGVLDNTIAVLGRITTVDELVAEWASKD"
     gene            9630..11147
                     /gene="fadD4"
                     /locus_tag="VGP27_23665"
     CDS             9630..11147
                     /gene="fadD4"
                     /locus_tag="VGP27_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD4"
                     /protein_id="HEV7854328.1"
                     /translation="MQIREHMDSAKPAIILSPSQTVIGFADLEARANRLAHCFREAGL
                     REGDTVAAIMENNEHVHAVMWAARRCGLYYALINTHLTAAEAAYIVDNSGARAVIGSR
                     ALRNVCEQLAEHLPDGLPDLLLIADDDLEGWRRYPDCVADQPSEPISDELEGDLLQYS
                     SGTTGRPKGIRRELPHLAPADAPNILAPLMTAVGITGDSVYLNPAPLYHTAPSYWSMV
                     VQSMGGTTVVMEKFDPERALECIQRYRITHGQFVPAMFVRMLKLPGAVRDPYDVSSLR
                     RVVHAAAPCPVEIKKQMIDWWGPIVDEYYASSEAVGASFIRAEDWLTHQGSVGRPLVG
                     IPHILDERGNELPPGEVGEIYYEGGHSFEYLKEREKTEASRDRHGWVTVGDVGYLDSD
                     GFLFLTDRRHHMIISGGVNIYPQEAEDLLVTHPKVLDAAVFGVPDDEMGQSVKGVVQT
                     VDPEDATEPFGEDLLIWLRGRLAHYKCPRSISFEPQLPRTDAGKLYKQELVKKYS"
     gene            complement(11155..12336)
                     /locus_tag="VGP27_23670"
     CDS             complement(11155..12336)
                     /locus_tag="VGP27_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854329.1"
                     /translation="MTTEQEDQVDTYGAPKLEWAKLPMAADRSVGWAAARDAGPVVYM
                     DGWFYLTRRDDVLDALRKPEVFSSKKAFDMLGSPLPLVPIAFDPPEHTRFRHVLQPFF
                     SPHTLGEMLPALQQQAVDIVGAVAARRECEVMADIAIPYPSQVFLTLFGLPLEDRDRL
                     IAWKDGVINLADRPSLEGVDLTPALELFSYLTDAINERRQNPDRDILSQVLTGEHALD
                     DAEAIGLSYLFVLAGLDTVTSSMGSALLELARNPELQAALRGDPEQIKVFIEEIVRLE
                     PPAPLVPRMTTEAVTIGDVTLPAGAQVRLLLGAINRDDSDEISTNHVVMDGKVHRHWG
                     FGGGPHRCLGSHLARMELTLIVDEWLKRVPAFELAPGYTPEIVFPANTFALASLPLRL
                     G"
     gene            complement(12351..13148)
                     /locus_tag="VGP27_23675"
     CDS             complement(12351..13148)
                     /locus_tag="VGP27_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854330.1"
                     /translation="MDYGISGTSALVVGGSKGIGFEVAKMLAAEGCRVAVLARTKADV
                     DAAVDTIRGAGGDAMGVCADVGQQEALENALAEMRTQIGPPLIVVGQAKYQRPGDFAD
                     IDDLDTYRESFESHTMSQIFLLRAVLPAMRDAGWGRFVHIGSATAKEPAGNIHHVVAN
                     TSRPSTIGLLKTVSDEYARYGITVNTVAPGWIETANSVAYLEKNLGAGTEEARREFML
                     GEARVPAARMGKADEIASLVAYLCSDHAGYITGSWIEVDGGLHRSAF"
     gene            complement(13165..13989)
                     /locus_tag="VGP27_23680"
     CDS             complement(13165..13989)
                     /locus_tag="VGP27_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854331.1"
                     /translation="MGSLDGRVVFITGAARGQGRSHAVTCAEQGANIVGIDICEDLDV
                     VPYKLGTHEDLEETARLVEKTGQEMLFRKADVRDKAALQEAFDGGVEQFGHIDTVIAN
                     AGVILTNADERDAEEALRIGIDIMQIGVWNAFQIAVPHLREQGGGNLIATSSMIALLD
                     MTDGRGGSDAYLMAKVAVLGLVRAYASLLAKDRIRVNAVVPTNCATPMITENPALFKV
                     IEEMPHLANAMQTALPDLPLIEPRDVSNAILFLISDAGRSFTGSHLKVDAGMDVRR"
     gene            14162..14989
                     /locus_tag="VGP27_23685"
     CDS             14162..14989
                     /locus_tag="VGP27_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7854332.1"
                     /translation="MSDTLEGGPRPPDGDWLGSPYLRFEREGAFAICTLDRPAARNAM
                     TPAMYFGIRYAINRVNADPELAGLLITGTGDVFAPGGDLGQRGEDNWMDFAAVMGMDV
                     TPFDVLRQSAKPVVAAVNGLCQGGGLQIAMCSDLAVVSERATFRVPELFRGIADTYYS
                     QMLARMIGPVRTRDLMFTGRTLSAAEALDWGMVARVVPHDELFDVSRELLAQCCRTAP
                     AARGVIKASLDNYMGLYDRIGMQTSLFGPETGEGFVAFKEKRSPSWVHPDLRVDGRL"
     gene            complement(15054..15833)
                     /locus_tag="VGP27_23690"
     CDS             complement(15054..15833)
                     /locus_tag="VGP27_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854333.1"
                     /translation="MTVQQFEGASAIVSGGAGGLGEATVRRLHADGLGVVIADLAADK
                     GKALADELGSKAIFVSTDVTSDDSVLGAIDQAQQLGQLRYAVVAHGGFGVAQRIVQRD
                     GSPADMGGFLKTIDLYLNGTYNMTRLVAASVATADPREDGERGAIVMTASVAGYEGQI
                     GQTAYAAAKAGVIGLTIAAARDLSSVGIRVNTIAPGTMKTPIMESVGEEAIAKFAANI
                     PFPKRLGAPSEYADAAAFLLSNGYVNGEVMRLDGAQRFTPK"
     gene            complement(15844..16938)
                     /locus_tag="VGP27_23695"
     CDS             complement(15844..16938)
                     /locus_tag="VGP27_23695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854334.1"
                     /translation="MTALAGGVFAASAAQDDDAELRSLVDELGQRSYDARLGRRGVPD
                     HFDAELWRHLDETGLTRLTSTPDMGAGPHELAIVLYGVARHAGAVPLAETDALAGWLG
                     QEAGIELPDGPLTVAIAEAETTDERVVGTAQGVPWARACSTVLAVRGADGLRVGVVDA
                     ATGDRQENHNLAGEPRDAIAFDVPTRELHSVDPALEAQLARRGAWARCIQIIGALDAA
                     AALTVAHTRGRVQFGRPLSAFQSVQQSLAGMAGDIERARAAVELAVAAAAEHGFDSPR
                     TDYAVTVAKIAVGRAVGPVTTTAHQLHGAIGVTSEHPLWLFTLRAQSWAGDYGTTAHF
                     ARRLGRLALAADDPWHLVTGDVTHLENNNG"
     gene            complement(16967..18115)
                     /locus_tag="VGP27_23700"
     CDS             complement(16967..18115)
                     /locus_tag="VGP27_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854335.1"
                     /translation="MSSFDELSANGPELVKLRASVRDFLQADRAEFAWKPAVDAWLGQ
                     WDEGFSARLGAAGFLGLTIPQRYGGRELGHLHRYVVTEELIAAGAPVAAHWTADRQVA
                     PSLLSYGSEEQRARLLPKIVAGKLYSSIGMSEHGAGSDLAAVSTKAVRAEGGWVLSGT
                     KVWTSGAHYAHQVVVLARTSPPDPEHRHDGFSQFLVPCDAEGVRVEPILLMSGAHHFN
                     EVIFDEVFVPDGDVLGEVGNGWHQVTAELAFERSGPERILSTGPLLLSAIRMLGEGPA
                     PDDQTAAAVGDLLARLISLRQLSLSVARTLTDGGDAANLAALVKDLGTRFEAESVELI
                     ADLLEGSTPNPELSAMLTTAWLHKPMFTLRGGTNEVLRGVVARGMGLR"
     gene            complement(18128..18985)
                     /locus_tag="VGP27_23705"
     CDS             complement(18128..18985)
                     /locus_tag="VGP27_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854336.1"
                     /translation="MLGPRTLKDRVVVVTGASRGIGREIAVRGASDGASVVLLAKTET
                     PNPKIAGTLTETAEAVRDAGGHAFPFAVDVRDADGVANAIAAAAEEFGGIDVVVNNAG
                     ALDLRPTPKLPPKSFDRLLAINVQGPFAVVQAAFTYLRNSDNPHIVNVSPPLNLDPRW
                     VGAHVGHTVGKYAESLLTLGWAAEFASIPIAVNSLWPATTVASTGMMVAMGEDVVRAQ
                     ARDPRIMADALHALVTRPAADCSGHFYTDEEVLRAEGVTDLSGYRLAATDDDLTPNFY
                     LPSTPLPAV"
     gene            19101..20327
                     /locus_tag="VGP27_23710"
     CDS             19101..20327
                     /locus_tag="VGP27_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7854337.1"
                     /translation="MTHVVSIGTYLPPWTVRERRVKGPDEDALTMAVAAGRAADPDAT
                     AARVVLVSRDFPLLEGGNGAVLLAALSLPADTPVTEVLGGAPAALDQLVSASPGTLVI
                     GADDNNLAAGAAAALTGGDGATVTGVARQTRSLPLTARGEDGTRHAYVDPRLQREVGV
                     RSTLARLGLTGTPTVAAVAGAPPGQLGTGFDTSDAAGDATPSAGAIIGLLAEAIEAGA
                     QGLLLAVEQASVSAAQLTLGAAKPRISRDEFSPQELPKTRVADGVGIPISMPAYARAF
                     EPKIRWEAAVFEESPGIDSAPQFPPRLRVNNAGELVDTYRLEALPRTGTVYTHTTVRI
                     PVPDLPSPYSLAVVQLDDSPVRVLLKVTGVPPGETTIGQHGAVVLRKIATRAGVPDYG
                     YAFWPGRTLSPEGVNA"
     gene            20401..21561
                     /locus_tag="VGP27_23715"
     CDS             20401..21561
                     /locus_tag="VGP27_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7854338.1"
                     /translation="MRNVAIVGAGMTPFAEHFELGVKDLVPMAYAEAVAGVDKGIDKS
                     EIQAAWFGELSTTDGFPSGILADTLDLTDIPVTRVENACATGNDAIRNGTMAIASGLY
                     DVVLVVGADKVRETSSNTTFWDWAAMTRDNAWDYPLGLVAPANFALHVIRYLHESPAT
                     KEHMAMVAVKNHFHALKNPKAQLRYEITVEQALAAPIVVEPFGLYDCTPQSDGAAAVI
                     LAAEEVVDRYTDRPVWVRGVGLGMDRVMHQHKSDMTTFPPTVRAAKSAMTMAGVTPRD
                     IDVAEVHDCFTGVELISYEDLGFANRFEAYKLVEGREHYVGGSIPVNPSGGLKAKGHP
                     PGATGVAQCYELFNQLRGQAENQVDGARIALAHNIGGPTAVSAVTILSTEKN"
     gene            21572..22381
                     /locus_tag="VGP27_23720"
     CDS             21572..22381
                     /locus_tag="VGP27_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7854339.1"
                     /translation="MTTSQVATLAGYEQFAPWLLVEKRDNVHVVSINRPEALNAVTEE
                     VHHAFATIWKVLDADNDVKAVVTTGVGKAFSAGGDMVMFGRLIEDEVARTFQIHEART
                     VFLEVINFPKPLVSAVNGPAVGLGCSIALLSDLLVMGESSYLADPHVAVGLTAGDGGA
                     AMLPLLIGMMKAKEYVLLGDRITAPIAEKLNLVTKVVSDDTVLDEALALGERLAALPP
                     QALRSSKVALNMHLARAAQGVLEYALAEELTSFSTPEFKERVAAFRARSKK"
     gene            22389..22670
                     /locus_tag="VGP27_23725"
     CDS             22389..22670
                     /locus_tag="VGP27_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HEV7854340.1"
                     /translation="MVTMKMRAAIVVTGMIAAAFGATAPAGADPTDDPCQLAVTFLCR
                     FLPMAPDLDHNVDLTKEPAIVDGQPLPEMPAGTQSQDTSPADICVDGCA"
     gene            complement(22725..23561)
                     /locus_tag="VGP27_23730"
     CDS             complement(22725..23561)
                     /locus_tag="VGP27_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEV7854341.1"
                     /translation="MTGVDEREVQQDIETPAAIQIRFGIEFLAASLADATVKMSMPMS
                     GMRNPFTGAPAVGPLAILVDAAAGMVNHYRRRTDQWTVSSELALEVSPNVAEALDDLA
                     EPVVASAGAVGSVGTTALSICTLTCGATVIGTGTVRSFFIPADGVTDKRPPQTLLGGT
                     PTLADLMAAQAEPTGDDSRVLKQHADPDLNNDIGIVHGGMASAALELAASAAINPDPS
                     AGFLKTASLRVNFQRPFLAGTASRYVGTPVRVGRSTGIADAVAIGDDGKIALSARITA
                     YR"
     gene            complement(23558..24298)
                     /locus_tag="VGP27_23735"
     CDS             complement(23558..24298)
                     /locus_tag="VGP27_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEV7854342.1"
                     /translation="MEVTSLREPKMADRVATVLRRMFIRGEITEGTMLPPESELMERF
                     GVSRPTLREAFRVLESESLIEVQRGVRGGARVTRPRRKTLARYAGLILEYEGVTLKDV
                     YDARVTLETPMVVQLAKDRNPGVIAELEAIVEREDQLSPGGDTVDQLTDFHAAIARLS
                     GNRTLQIVSDMLHHIIEKANRSLQPTEGTRAAGAVTRSVKTHRMVLDLIKAGEAEQAG
                     ELWKKHLQKAEAFVLSGAELSTVVDLLE"
     gene            complement(24467..25609)
                     /locus_tag="VGP27_23740"
     CDS             complement(24467..25609)
                     /locus_tag="VGP27_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854343.1"
                     /translation="MRRNIFEEIHDDFRATARSFFERECVPNVEKWERDGKVSREAWL
                     AAGEHGLIGWEFDEKYGGLGVKDFRFNQIISEEMFLTGSVGIGLGVQNDILMPYMNNL
                     TTDEQKERWLPKFVAGEYIGSIAMSEPAAGSDLAGIKTTARDEGDHWVVNGQKTFISN
                     GLLSKLVLTAVKTDPAARHKGISLLMIEDGMEGFTRGRKLDKIGQYSADTAELFFEDV
                     VVPKENLVGELNRGFYHLVGNLPSERVGVACYALPAARRALDLTKAYALERTAFGQPI
                     GKFQVNRHFLAEMQTKLDAAQSYLDQCVLSVNDGTLSDQDAAGLKWWTSEVQWEIIDR
                     CLQLHGGYGYINEYEIARLWRDSRVQRLYAGTTEIMKDLMGRAMGY"
     gene            complement(25618..26610)
                     /locus_tag="VGP27_23745"
     CDS             complement(25618..26610)
                     /locus_tag="VGP27_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854344.1"
                     /translation="MRTRGANPQDEAWPMVEEAVFRLFEELAAKQEHTVVGPRLTEMG
                     WAEIESEYPVEACALLFRAQGHSLALTDALDRVMLAELASLLDAPADTIVLPGLSHGY
                     APSSSEYQVKGIVLGPLRGRVVVPVSRAMGTVSIAVVDADELRGEPMDTFDRSVVWTR
                     VEGTTDDALIDASTEWGRAIAAAHRSIATELIGLADQALRIAVEHISVRVQFGSPIGS
                     LQSPRHALADAAAQLEGARALLNESWCYGGILSAQTAKIAAGRAHRTVSDAVLQVCGA
                     IGLTAEHDLHRYVARGFQIDALCGSYLQLESLLAERLFDTYMPGRALPAMVNWN"
     gene            complement(26610..27830)
                     /locus_tag="VGP27_23750"
     CDS             complement(26610..27830)
                     /locus_tag="VGP27_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854345.1"
                     /translation="MSFPVNNGVSGGRRDAYLSQVAEYRQDLREYLRTLDCAEQWRSA
                     ASMTAEQGLEHDASVMARLHADGWNRYGWPEAAGGFGGNEVHRAVYFEELGHAMLPIP
                     AQAWTLEVMGPAVLEFAPQLAAEYLPGYLDGSEWWGQGFSEPESGSDLASLRTRAADD
                     GAGGFVVNGQKIWTSQGGTAKRVCVLARTGSPESRHRGLTMLLIDTDTPGLTIRPIAL
                     ASGRRELAEMYFDDVRVPRERLVGDVDKGWAVTMFLMQYERGMYGYAVLNKVLTELGR
                     LREYMVTSNATAAQRERFARVYVDVIAAQARAATTVRKLAAGEPVGPESSIDKLLCSR
                     AEREVNDLILDVARDYLLAGSPPGVNREAELDTARAEWWYSRAATIMGGTAEVQRGIV
                     ADHILGLPKERRAS"
     gene            27942..29444
                     /locus_tag="VGP27_23755"
     CDS             27942..29444
                     /locus_tag="VGP27_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854346.1"
                     /translation="MTASPVAATTVQFNPESRLYIDGELREASTGKTADNINPATEEV
                     LGVTADADAVDIDEAIAAARRAFDTTDWSTNHEFRQRCLMQLHEALQEEKEDARAELV
                     AEAGAPLGMTYIAQLDWPLADAVRWPAQHISEFAWERMLDVDAKMGEPYNRVVVKEPM
                     GVVGAITPWNFPVEIISNKVGQVLATGNTMVLKPAIETPWSALRWGRIIAEKTDIPAG
                     VVNIVTASDNDVAQRLVTDPRVDMISFTGSTAVGELIGRLSAATMKRNLMELGGKSVY
                     LLLEDADLATTVPGCIGALMHSGQGCALTTRMLVPRDRYDEAVELATATFASVTVGDP
                     TDPANFCGPLVSAKQRDRVLGYIEVAKEQGARVTVGGGKPEGLDRGYFVAPTVLADVA
                     PDHRVFQEEIFGPVITITPYDGGDDGAVEMANNSIYGLAGTILGSNQRAMAVARRIRT
                     GSVCVNSGIFYGADAPFGGYKMSGLGRQNGVEGFEQHLQTKVIAYPLETA"
     gene            29441..30652
                     /locus_tag="VGP27_23760"
     CDS             29441..30652
                     /locus_tag="VGP27_23760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854347.1"
                     /translation="MSDILSGIRVVELAAWTFVPAAGAVLADWGADVIKVEHPETGDP
                     QRGLISSGLVTGAGGVNHFIEQPNRGKRSIGLDTSTPEGLELLMKLIETADVFVTNLL
                     PDSRQRMGIDVEQVRARNPKIIYARGHGYGTRGDLASQGGFDLAAYWARGGIGEGYAA
                     GDGSYPPIQRPAFGDSYGGLAIAGGIAAALVKRERTGEPSVVDVSLLNAAIWQLGPDI
                     VGSGVTGQDIPKFNLDEMPNPAASIYKTRDSRFIAFVLLQADRFWADFCTRVGRPDLI
                     DDERFANAAVRFENRKDCIAELRKAFAAEDLSHWEKAFAGFDGVWDVMRTAHELHADP
                     QVIKNGYLPRVTNADNNEFALAASPVQFDETPLDLTCAPGHGEHTDALLAELGYNEDE
                     IIDFKIKSVVL"
     gene            30652..31602
                     /locus_tag="VGP27_23765"
     CDS             30652..31602
                     /locus_tag="VGP27_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854348.1"
                     /translation="MVLEQLARRFLHVNLNCDSLDATEGLYVDKLGLSARMRTDPEVA
                     TDGSILGLDGETFCATSFLYDSRGGRGGCALEAIEYRSPTLKRDPSSDPARPGIRSTL
                     MTVEDVGDTVSKLREAGVTVSDPVDGLISGTKSVLASDPDGAVIELAESAPDVKASDG
                     ALFGGIRISAINAVATGEFLTAIGFAEVEAPTLVKVAGDQMDPGGSSDAVECVVSRYA
                     VPEDAHQFSLVVVQHPETRSQDPVPWGGNHQGLYRCAMRVDSAEQALAAVPDSVEVMG
                     DPVWCPLPGTKIEGLYIAFVRSPDGVVFEFVERPLAHFTG"
     gene            31616..32437
                     /locus_tag="VGP27_23770"
     CDS             31616..32437
                     /locus_tag="VGP27_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854349.1"
                     /translation="MQQLSGKVALVTGAARGQGRSHAVSLADEGADIIAVDIAADIES
                     IPYALGTKEDLDETAQLIRNTGRRAATYVTDVRHLGELQACVQAGIAELGDIDIVVAN
                     AGVVATGRTDPFDEQVYRDIVETNLFGTWNTIVAAVPSIIRKGRGGSVILTSSTQGLV
                     GRGGDGSAAAFGYASSKHGVVGLMRSAAYAYAEHNIRVNTVHPTGVATPMVLNEHMGR
                     VFQENPSAPKLATNLLPVPFIEAQDVTNLVVWLASDKARYVTGATMPVDAGYTAL"
     gene            32446..32868
                     /locus_tag="VGP27_23775"
     CDS             32446..32868
                     /locus_tag="VGP27_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7854350.1"
                     /translation="MTQAAGLIEFLRHNRRAFLFCRDESGRPIGYAMQSVGCDRTSLY
                     FSTYTNSPKVKHLRADPEAACIVLSGQDVMDQPWVSLRGSAEVYRPSVDEIDEMIGAG
                     TPDGRVPHAVVAKVRDRLISGKRCFIRLVIDEVCAHAT"
     gene            32891..33220
                     /locus_tag="VGP27_23780"
     CDS             32891..33220
                     /locus_tag="VGP27_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854351.1"
                     /translation="MSADELASFLEQRPTGAICVTDSAGRLVALPAAVLGQEDDTLKV
                     EIDGLDEPLVPGTAVCVVADRFPSYEAIRGIITQGSITSTEHVRPPSVAVAVTRTVTF
                     SFANVIT"
     gene            33299..34147
                     /locus_tag="VGP27_23785"
     CDS             33299..34147
                     /locus_tag="VGP27_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEV7854352.1"
                     /translation="MLATLTDVLNLFDLSTLAPDIFEGNQPDPPNHHIVGGQIAAQAL
                     MAASRTAEGRPPHSLHVYFLRRGDARWPVRFEVTRLHDGGRFSTRRVAAVQDGAVLME
                     GLASFTAGVEAADYQRAMPDAPEPESLVPVDEQLAPYADEFGDWWGTERPFDVRYVDP
                     PPRVAADRKQPAGPVSRIWLHANGSVPADPVVNSCVLTLLSALTLLEAALGPMRKSQR
                     DVSALLDHTVWFHRPADFSDWLFYEQNSPSGIAGRALSTGTIYNRRGDLVCTAGQEGY
                     FPPARV"
     gene            complement(34156..35067)
                     /locus_tag="VGP27_23790"
     CDS             complement(34156..35067)
                     /locus_tag="VGP27_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854353.1"
                     /translation="MTGPLSGRVVLVTGGGRGIGRGHCLALSAQGASVVVNDPGVSRD
                     GTAGDGAGPAADVVAEIEAAGGKAVSHTGSVSSWDDVADMIGTAVEAFGSLTGVVNNA
                     GIVRDAMVATSTEDDWDAVIAVHLKGTFAVTKHACEYWRAQAKAGNQIDARIVNTVSG
                     AGLWGNVGQSAYGAAKAAIANLTVVTAMEAGRYGVTVNAISPLAVTRISEDFFSGERA
                     DDPALDPARSSDVVAWLQSPDSSWLTGQILRVNGDTLSRIEGFTEAAGRYHAKDGVSL
                     RFSEIGQAVSWLYGTSPRGLRGPLPTA"
     gene            complement(35076..36251)
                     /locus_tag="VGP27_23795"
     CDS             complement(35076..36251)
                     /locus_tag="VGP27_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7854354.1"
                     /translation="MSTQLAAALRTRVRPGGTVAIGDGVGALRCLDDGTSVGAAISAL
                     ARDVGSVRLILGWLPTSLDGIEADAFAETVALMPGWGVRTVLASPTARFVPTRLSAIP
                     SLLTGMLRPDVLVTRVVERAGRLHFGTEVAWQRGLVDAGVPVLAVVDNTSPCADAGPP
                     LDPAAVHVCGRSSDGPARMPQRDPEPIHDALADAVLRFVPEDARLQYGPGQLGTALLR
                     RAAVPLRIDTGMLTDAVVDLDKRGMLVDMPSATYLFGSEVLYDWADGKPILRGIDYTH
                     DLSRLSRGEPFVAVNTAIEIDPFGQVNVEGRGEKVVGGIGGHPDYCAAGRMSHGGLSI
                     IAVPTTVNGRSPLVDQLSRPVSTPAHDVDLIVTESGHADLRGADWPQRRRLIATLFQ"
     gene            complement(36320..>37109)
                     /locus_tag="VGP27_23800"
     CDS             complement(36320..>37109)
                     /locus_tag="VGP27_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854355.1"
                     /translation="PIRLGDDIWTQLFSEPGAGSDLAGVRTKAERTPTGWRINGQKVW
                     SSAANSADYGLLLARTGPDKHRGLSMFVIPMDTPGVSVRPLTQMDGESKFNEVFFDDA
                     ELEDDALIGEVDQGWAVAIMTLGRERLTLGSQAVGMFRLHERMVDAARDHDLLDPVLA
                     RSMTKLWARMWLLRYTWQRAIDAGDTSSSAFSVLKLMTSETDRDLGDLATEVLGTDAC
                     ADPADNELVQHMLVGRAQTILGGTSEIQRNILGERVLGLPKEPR"
BASE COUNT         6554 a        12163 c        11982 g         6410 t
ORIGIN      
        1 ggtggccgga gtcgtgctgg cgatcccgct gtactgcctc gggctgatgg ccgcctacct
       61 cgcgacccgc ttcggcacag ttcttggtta cgggcagtcg acgggcgtct acgaccacta
      121 cttcgacact tttttgaacc ctacggacgt agtgctgtcc ttcacccagg caatcggaat
      181 ggcgaccatc gtgatgctga tacacactta ttacggttac accgcatcgg gcgggcccgc
      241 gggggtgggc gaggccgtcg gtcgcgcggt tcgcgcatcc ctcgttgccg tcatcgtcgt
      301 tctcctgttc gtcgcgctgg gcatttacgg ccaaggcggc ggcctgaata tttccgggta
      361 gcgggatggc ggcagcagcg cgaaccaaaa aacgagcggg ggaatcacgc atccacccgg
      421 ggtggtggac cctcggtttg atcctggtgg tcgtcggcct tgttgtgctc gacgccgtcc
      481 tgttcaccgg cagctaccgg tcctccatcc cggtcacggt gatgtccgat cgttcgggac
      541 tgatcatgga aaccggtaac gatgtgaagc tgcggggcgt cgtggtcggc cgggtggagg
      601 gtatctcgaa gggcaaccca ccggttggcc tcaacctcgc tatcgattcc gctcaggtga
      661 aatacattcc ggccaacgtc gaggcggaga tcaaggcgac gacggcgttc ggcaacaagt
      721 tcgtcgacct cgtcgtcccg gatgacccca gcgccaagcg gctctcggcg ggtgaggtgc
      781 tcagggcgcg gaacgtcgcg accgaggtca acaccgtgtt cgagaatctc gtcggagtgc
      841 tccagcaggt ggacaccgcc aagctgaacg ccgtgctgtc cgcgctggct gagggcgtgc
      901 gtggccaagg cgagaggctc ggcgaagcga tcaccgccgg taatgaggtg ctgatgcaac
      961 tcaatccccg cagcgagacg atccgccagg actggaaggc ggtcaagggg ttcagcgaca
     1021 cctacagcgc ggcggcgcag aacatcatca ccgtgctcga cgcggccagc accaccagca
     1081 ccacgatcac caaccacgcg aagcaactcg actcgctgtt gctcaacgtc atcgggctgt
     1141 cgaacagcgg cgtcaacgtc ctcggtccca gcaaggacaa tctgatccac gcggtcaacg
     1201 tgctcgattc cacgaccagt ctgctgatga aatacaaccc ggaactgacg tgtctgttca
     1261 ccggcggcaa gaacgtcatc gacttcgggt tcgagaacgt cgcgggcggc agcaccggta
     1321 agtcggtcat cctcgacgtg gcgttgctgt tcggtgacga ccagtacaag tacccggaca
     1381 acctaccgat caacggcgcg aagggcggtc ccggcggcaa gccaggctgc ggttcgctac
     1441 ccgatgtcgc tcagaactgg ccgcaacgcc aactgatcac caacaccggg tggggcaccg
     1501 gcgtggacat tcggccgaat ccgggcatcg gcttcccggg ctacggcaat tacttcccgt
     1561 tcacccgtgg ggtgccgcaa ccgccgacca tgaatcgcat cggaccgccg gcaccgggac
     1621 cgatcccgta ccccggggcc ccgccgtacg gtgcgcagca atatgcaccg gacggcaccc
     1681 cgctctaccc gggtttgccg cccgcgccac cgccggggca accaaaggaa ccgggacccc
     1741 gggcgggaga agagccgttc gtggtgccct atcccgcaca ggtgcaaccg aatccaccgc
     1801 cccccatacc accggggccg gtcaccccgg cgccctgacc agaccagcca gatgagaaag
     1861 cagacgatga gagaccaccg ccatcatgga tagcttgcgc ggcaccctcg tgcgcatcgc
     1921 catattcctg gtggtgtgca cgctgggctg gtttgtgacg gtggcggtct tcggacagtt
     1981 gcgcttcgac gaacaaaaca agtacaacgc cgtcttcaag aacgtctcgg gactggagac
     2041 cgacgacttc gtgcgtatcg ccggagtcga ggtcgggaag gtcaagggca tcaaggtccg
     2101 agacgacgcc acggtggacg tcgagttcgc cgctgacaag tcggtggtgc tcaccgaggg
     2161 cagcagggta gcgatccgct acgagaacct caccggggac cggttcgttg cgctcgagga
     2221 aggcgcaggc ggaacgagga agctgaacgc gggcgacacg ataccgctgg agcgaacgga
     2281 acccgcactc gatctcgacg ccttgatcgg aggattccgt ccgctcttca acgccctgag
     2341 ccccgatcag gtcaacgcgc tgaccggcca gctcatcgag gcgtttcaag gccagggcgc
     2401 gacgatcagc tcgtttttga gtcagactgc ttcgctgacc aacactctgg ccgaccggga
     2461 tcagctgatc ggacaggtca tcgtcaacct caatacggta ttgggatccc tcggcgacca
     2521 gaaagagcag ttcggcaggg ccgtcggctc gttgtcacag ctggtgcaag gcctggccga
     2581 acgaaagcag gacatcacca acggtgtggc gtacgccaat gcggccgcgg ggtctgtcgc
     2641 ggacctcctg cagcaggcgc ggccgccctt gaagaaggtc gtgaccgaag cggaccggct
     2701 ggcaggcaac atcgtcgcgg acgccgacta cgtcaacaat ctgctcgata cgctgccgga
     2761 ctcatatcgg attctgaacc gtcagggcct ctacggcgac ttcttcagct tctacctgtg
     2821 tgatctcgtg ctcaagctca acgggaaggg cggccaaccg gtgtacgtga agttggccgg
     2881 ccaggacacg gggaggtgca ctccgcgatg aagtcctttc aagagcgcaa cccactcatc
     2941 atcggtgcca tcggcctcac tctgtccgtc gctgtggctc tcggcgcggt taattacgac
     3001 aagctgccgg gcctgtcggt gggcaaggag tattcggcgt acttcgcaga ggccggtggt
     3061 ctgctgtcgg gcgccgatgt gcgggtgagt gggttcgccg cgggcaaggt cgaagacatc
     3121 acgctcgatg actcgcgcgt gctggtgacc ttcaaggtcg acgacgacat ccgcctcggc
     3181 gagcgcagcg aagcggcggt gaagacacgg agcgcattgg gcagcaagat cctcgagatc
     3241 accccgcgcg gggacggcca gctgtccggt ccgatccccc tcgagaggac gacgcccgcc
     3301 tatcagctgc ccgatgccat cggagacctc accaccgcca tcagcggcct ggacacgaac
     3361 cagttgtcgg attcgctgaa gatgttgtcg gagacattcg ccgacacacc accggacgtg
     3421 cgcgccgcgg tggaaggggt gggtcggttc acgcagaccc tggacgagcg tgacgcgcag
     3481 ctacgaagct tgttgtccaa tgccaacaag gcgactgcag tgctctcgga gcgcagcgac
     3541 cagatcgtca ccctcgtccg agacacgaac gcgctgctcg ctgcattgcg aagcgagagc
     3601 gcgtcattgg accagatctt caacaacatc tcggccgtga gccagcagtt gaagggtttc
     3661 atcgccgaaa accggacaca gttgaagccg gctctggaca aactcaacgg cgtgctcgcg
     3721 atcgtcgaca accacaagga acaggtcaaa ctggcgctcc agggactcaa tcggtacgcg
     3781 ctgggccttg gtgaatcggt ttcgtcggga cccttcttca aggcttacct ggtcaacctg
     3841 ttccccggac agttcgtcca accgttcatc gacgcggcgt tttccgacct cggcctcgac
     3901 cccaacgtgc tgttgccgac acaacgcagc gacccacagg tggggcaacc gggcacgccg
     3961 gcactgccga tcccgtaccc acgaaccggc cagggtggag atccgaaatt gacgattccc
     4021 gacgccatca ccggaaatcc cggcgatcag ggttgcggtc cgccgggagt gccgttgccc
     4081 ggcccgacgg gctgttaccc gtaccgcgag ccgttgcctg cgccgccgcc gggcggcccg
     4141 ccgccaggac ctcccgcagc ggcatccccg gggcaggagt ccaccgccac gccgccgctt
     4201 ccgatctacg cgcctgcacc cggtgagcag gtaccggcag gcgcccaaag ccggccaccc
     4261 ggcgacccgg cggtggtagg acccggcgca cctggagcag ccgacgtgac tccggtgccg
     4321 ctcggcccgc cgccagggcc gcccgcaccg gcgggagacg gccagtgaac aggctcaact
     4381 cgcggacggg aaagatcggg ctcgccgtag ccctggtggt gttgttggtc ctcgcaggcg
     4441 tgacgatctt tcgaagtgga gcagcgagca agaccacgat cgtcggatac ttcgacaaca
     4501 gcaacggcat ctacacgggc gacgacgtgg tgattctcgg cgtgcctgtc ggctccatag
     4561 acaagatcga accggagccg ttccgcacca agatcacctt ctcggtcgac gacaagtaca
     4621 aggtgcccgc cgacgccaaa gccgtcattc tctcgccggc gttggtgacg gctcgcgcca
     4681 tccagctcac gccgacttac aagtccgggc cgaagatgca ggacggcgcg gtgattccgc
     4741 aggagcgtac cgccgtaccg gttgagttcg acgcgttccg ccagcagcta gaacgactcg
     4801 ccgacacgtt gcagcccacc acgccgggcg gcgtgagcac ccttggcgcg ttcatcaaca
     4861 ccgctgctga caatctgcgc ggtcagggcg cgaacatccg tgacacgttg atcaaactgt
     4921 cgcaagcgtt ttcggcgctc ggtgataaca gcaagggcct tttcggcacg gtcaagaact
     4981 tggcgatatt ggtgtcggca ctgcgcgaca gtagcgacct gctacggcag ctgaacggaa
     5041 acctggcctc cgtgacgggg cttatctcca acgaccccaa cgaggtcggc aacgcggtga
     5101 aggacctcgc tgacgtcgcc ggcgatgtca gaagcttcgt cgccgagaac agggagaccc
     5161 tcggcaccac ctcggacaag ctcgcgtcgg tgacgcagaa cctggccgac aacatcgacg
     5221 aggtcaagca gttgctgcat gtcgcaccca acgcgttcca gaacttcctg aacatctacc
     5281 agcccgcgca gggcaccctg accggagcgt tggcgttgaa caacttcgcc aaccccattc
     5341 aattcctctg tggggcagtg caggccgcct ctcggttggg cgcagagcag tccgcgaagc
     5401 tgtgcgtgca gtatttggcg ccgatcatca agaaccgcca gtacaacttc ctcccgttcg
     5461 gcctgaaccc attcgtcggt gcgaccgctc gacccaacga ggtcacctac agcgaagact
     5521 ggatgcggcc cgactacatc ccgcccgccg gtgcggggcc ggcgctgcct gcagaagcgc
     5581 ccggatcgca agcaccttca acgccaccga gcggtggacc cctgccgccg ccgggcagtg
     5641 ttttcaccaa cttcggtgag cccgccgatc ccaatcacgg tcagccgccg ttcgtgccgg
     5701 ccaggccacc cgatccaccg ccactcaacc cgggcgacgc tccggccgta cccgcgccgg
     5761 tctccactga tccgggcacc gggctgaccg gcatgatggt tccgcctggg ggtggccaat
     5821 gagcggaaat cacctgcggc ggatactgct tggcgtcgtc gtcgtcgtgg tgatggcggt
     5881 gccgggctgc gccaactggc ggggcctcaa ctcaataacg ttacctggga cggcgggcgg
     5941 cgaggccggg gcctacacga tcaaggcgca actacccgag gtcgtcaatc tgcagccgaa
     6001 ttcccgggtt cgggtcggtg acgtgacggt cggcaacatc accaagatcg agcgccagga
     6061 ctggcatgcg ctggtcacga tgacgatcga cgggaacgtc gacctgcctg cgaacgccac
     6121 cgcaaagctc gggcagacaa gcctgctggg atcactgcac atcgagctcg ccccgcccaa
     6181 agaggttcca ccgcaaggca aactcaagaa cgggtcgctg atcccgctgt cgagtgcggg
     6241 cacctatccg accaccgaag agacgctcag tgcgctgtcg ttgctgctga acggcggcgg
     6301 tgtcggccga atccaagaaa tcacgaagga actgagcacc gcattctccg gccgtgccga
     6361 cgacctcagg agcctgctgt cgcaattgga tgtgtttgtc aaatacctca acgaccaaaa
     6421 ggacgacatc ctggccgcgg ccgacagcct gaacaatctg gttggtcaga tcgccgacca
     6481 gaaacctgtg gtcgacaagg cgctgaagac ggttccgcag gcgctcgcga ttctcaaaga
     6541 cgaacggatc accctcgccg atgcgctcga ccagctcggt aagttcagcg cactggctgc
     6601 cgattcggtc aaccaaacga aggaaaatct ggtcaaggag ctcaaagacc ttgggccggt
     6661 actggaatcg ctggccaacg cgggccagga tctcacccgt tcgctcgact tctatgccac
     6721 cttcccgttc cccaagccga cactgaacaa gtggttgcgc ggcgattacg cgaacttgac
     6781 cgccgtcatc gacctgaccc tcagccgcct ggacgcgtcg ttcttgaccg gcacgcgcgc
     6841 cgaaggcgaa ttgaccgagc tggaattgca gtggggccgc accatcggcc agatgcccag
     6901 cccgtatacg gcccgcaatc cgctggtcgc tccctaccgc ttcgatcagg ggccgtgaca
     6961 tgaacatcac gaggcgaatc tggattcagc tgggcgtggt gtccctggtg tcactcgccg
     7021 gtgtcttgct gatgtttttc ccggtcctgc atctgcagga acggtggttc ggggtcgggc
     7081 gctacacggt gacggtgcag ctgccgcaga cgggcgggct gtacgagcgg ggcaacgtca
     7141 cctaccgcgg gtatcaagtc ggccaggtga aatccgtgca gctgaccaat atcggtgcgg
     7201 aggcggtgct ttcgctcaac tcggacgtga agattccctc ggacgtgaac gccgaggtgc
     7261 acagcgtgtc agcgatcggc gagcagtaca tcgatcttct tccgcgcagt gccgacggtc
     7321 caccgttgaa ggacggctct gtcataccgg tcggacagac gtcggtgccg ccgaacatca
     7381 gctcgctgtt ggacgccaca aacaaagggt tggaggcgat tccacgcgac aacctgaaga
     7441 ccgtggtcga cgagtcgtat atcgcgttcg gtggtctggg acccgagctg gcacggttcg
     7501 tgaagggcgc cacggctctg gccatcgacg cgcgggccaa catcgacgac ctgacgaccc
     7561 ttgtcgacca atccaaaccg atcctggatt cgcagatcga ctcatcggac tccattcagg
     7621 catgggcgtc gaacctcgcg acggtcacca gtgagctgcg cgcccaggac ggcaatctcg
     7681 cgacagtgct gcaggatgcg ccgggggctg tcgacgaagc tcgtgcgctc ttcgatcggc
     7741 tgcagcccac gctgcccatc gtgttggcga accttgtcac cgttggtgac gtcgccgccg
     7801 tgtatcgcga caacctcgag cagctcttgg ttctcctgcc acagggcgcg gcggctgttc
     7861 aggcgtccgg ggtggccaac cggaacacca agcaggatta caacggcgcc tttctgagct
     7921 tcaatttgaa cctcaacctg ccgccagcat gcacgacagg gtttcttccc atccagcaac
     7981 agcgggccgc cgcgttcgaa gactacccac cgccgccggt cggcgatctc tattgccgcg
     8041 tgccccagga ctcgccgttg aacgtgcgtg gggcgagaaa ctcgccatgc gtggagaagc
     8101 ctggcaagcg cgcgccgacg gcgaagatgt gcgagagcga cgagaactac gtgccgctca
     8161 acgacggcta taactggaag ggcgacccca acgccacgct ctcgggtcag ggtattccgc
     8221 agccgcgtga tccgtcagcg ccgggtagcg gcccggtcga gggcgtaccg cccgcgccgg
     8281 cacccgcacc gccgccggtg gctttcgccg agtacgaccc cgccaccggt acctatgtcg
     8341 gacccgacgg tcaaaagcat acccaagcaa acctgggcga agccaaagga aagcagacgt
     8401 ggcaggacat gctgacaccg ccgacggggg gctgatgagt gagtcagccc cacaaacagg
     8461 gcgcgcccag caggcgattg gtggagggcg ggttcagatc gtcgaactga tcggaccggt
     8521 ggtgcggcaa gccggctgca aggacttcgt tatctcctgc cagcatccct cgccggtggc
     8581 cgtgaaggtg ccgctggcgg gggatgccgg gatgatgacc tccgcttttg cccatcccgg
     8641 catcttcttg caatgccccg gactgttggg tgtcgacaag cacaccgtga cgtcggtcgg
     8701 ctgcgccgcc gggtcacagg ttttcggatt gagggtggct ctgaccgctt tggcgccgcc
     8761 gggtagcaca accagttcgg gtggcgtcaa tatgtattgg cagcctgcgg cgtccgacga
     8821 ttcgtcgacc ggcacggctg acgctatcgc cgtggcaccg cccaaggcac ttgcaaaggt
     8881 cgcggccgcg gcagcagatg cgaacatcga acggagcaca atcatcggca tagtttagtc
     8941 cttcgaggcc cattcggcga cgagttcgtc gactgtggtg atccgaccaa gcaccgcaat
     9001 cgtgttgtcc agcacgccat ttgcgtagtc cattggtgtc cctccgactg cgtcgcgtgg
     9061 aacgacgagg cggaatccgg cttgggtggt gtggccggca gtggtcggca acgcaagatt
     9121 cagcgacact ccggtgagga ccacggtgcg cacgcccagt cccttgagca ctggcagaag
     9181 ctcggtatcc agggtgggga acagcccgtg atggcgtggc agcaccatgt cggtcggcgc
     9241 gagaagctct ggtaacactt gtgtttcgac gcggcccggt gcccaggccg cggtagcggg
     9301 ccctagcgcg cgccacagtc gcgcggtgcc cgtcgggtca ccgccaagcg atccctcata
     9361 ggtggcgtga accactcgga cgcctcgctc acgggctccg tcgagaagac ggcgcagatt
     9421 tgcgaccagg cccgcgctgt cggcggctaa tgcgggcaac acagaatcgg ggcccagcac
     9481 gccgttctgg cactccacac agacaacggc ggtggcggcg agatcatggt cttgcggtgt
     9541 tgatggcatc gactatcact ctaggggaaa gctgttagca ggaacagggt ttcgcttcga
     9601 tgacggccgc caactcgtac agtggaggag tgcagattcg tgagcatatg gactcggcca
     9661 agccggccat catcctgtct ccgtcacaga ctgtgatcgg cttcgccgac ttggaggcac
     9721 gggccaaccg gctggcgcac tgcttccggg aagcaggtct gcgtgagggc gacacggtcg
     9781 cggccatcat ggagaacaac gagcacgttc atgcggtgat gtgggcggcg cggcgctgcg
     9841 ggctgtacta cgcgctgatc aacacccatc tcaccgcggc ggaggcggcc tacatcgtcg
     9901 acaacagcgg cgccagggca gtgatcggct ctcgcgcgtt gcgaaatgtc tgcgaacaac
     9961 tcgccgagca tctgcccgat ggcctcccgg acttgctgct gatcgccgac gacgatctcg
    10021 aaggctggcg gcgttacccg gactgtgtgg ccgaccagcc gtcggagccg atcagcgacg
    10081 agctggaagg cgatctgctg cagtactctt cgggcaccac cggtcgtccc aagggcatcc
    10141 gccgtgaact cccgcacctc gccccggccg atgcgccgaa catattggcg ccgctgatga
    10201 ccgcggtcgg aatcaccggc gattcggtgt atttgaaccc ggcgccgctt tatcacactg
    10261 caccgtccta ttggtcgatg gtcgtgcagt cgatgggcgg caccactgtg gtgatggaga
    10321 agttcgaccc cgaacgtgcg ttggagtgca ttcagcgata tcgcatcact cacggtcagt
    10381 tcgttccggc gatgttcgtc cggatgctca aactgcctgg tgccgttcgt gacccgtacg
    10441 acgtatccag tctgcgcaga gtggtgcatg ccgccgctcc gtgccctgtg gagatcaaga
    10501 agcaaatgat cgattggtgg ggcccgatcg tcgatgagta ctatgcctcc tccgaggcgg
    10561 tcggtgcgtc attcattcgc gccgaggact ggctcacaca tcagggatcc gtgggtcgcc
    10621 ccttggtcgg catccctcat atcctcgatg agcgtggcaa tgagctgccg ccgggcgagg
    10681 tcggcgaaat ctattacgaa ggcggccact ccttcgagta cctcaaggag cgggagaaga
    10741 cggaagcgtc gcgcgaccgg cacggctggg tgaccgtcgg tgacgtcggt tacctggatt
    10801 ccgacggctt tctgtttctg actgaccgcc gccaccacat gatcatctcc ggtggtgtga
    10861 acatctaccc gcaggaggcc gaggacctgt tggtcacgca tccgaaggtg ctcgacgccg
    10921 cggtgttcgg cgtccccgat gacgagatgg gtcagagcgt gaagggcgtt gtgcagacgg
    10981 tggatcccga agatgccacc gaaccgttcg gcgaagacct actgatctgg ctgcgaggac
    11041 gcttggcgca ctacaagtgt ccgcgttcca tctcgttcga gccgcagctg ccccgcaccg
    11101 acgcaggcaa gttgtacaag caggaactgg tgaagaagta ctcctgatcg ccggtcagcc
    11161 caaccggagc ggcagactgg cgagcgcgaa ggtgttagcc ggaaagacga tttcgggagt
    11221 gtaacccggc gccagttcga acgcggggac ccgcttcagc cattcatcga cgatcaaggt
    11281 cagctccatg cgggccaggt gcgaacccag gcagcggtgc gggccgccgc cgaatcccca
    11341 gtgccggtgc accttcccgt ccatcacgac gtggttggtg gagatctcat cgctgtcgtc
    11401 gcggttgatc gcacccaaaa gcagtcgaac ctgcgctccg gcaggcagtg tcacgtcgcc
    11461 gatggtgacg gcctccgtcg tcatcctggg caccagtggc gctggcggtt ccaaacgcac
    11521 gatctcctcg atgaagacct tgatctgttc gggatcgcca cgcagcgccg cctgcaattc
    11581 ggggttgcgg gcgagttcca gcagtgcgga acccatcgag gacgtgacgg tatcgagtcc
    11641 ggccagtacg aacaggtagc tcaggccgat tgcctcggcg tcgtcgaggg cgtgctcccc
    11701 ggtgagcacc tgcgacagga tgtcgcggtc ggggttctgt cggcgttcgt tgatcgcgtc
    11761 ggtgaggtag gagaacagtt cgagcgcggg ggtgaggtcc acgccctcca gcgacggccg
    11821 gtcggccagg ttgataacgc cgtctttcca cgcgataagg cggtctcgat cctccagcgg
    11881 gagcccgaac agcgtcagaa acacctgcga tggataggga atcgcgatgt cggccatcac
    11941 ttcgcactcg cgtctggcgg cgacggcacc cacgatgtcg accgcctgtt gctgcagtgc
    12001 cggcagcatc tcgcccaacg tatgggggct gaagaacggc tgaaggacgt ggcggaatcg
    12061 ggtgtgctcg ggcgggtcga aggcgatcgg tacgagcggc agtggacttc cgagcatgtc
    12121 gaacgccttc ttcgacgaga agacttcggg cttccgcagt gcatccagca cgtcgtcgcg
    12181 acgggtcaga tagaaccagc cgtccatgta cacgacgggc ccggcgtcgc gtgcggccgc
    12241 ccacccaacg cttcggtcgg ccgccattgg caacttcgcc cattcgagct tcggggcgcc
    12301 gtatgtgtcg acctgatctt cttgctcggt ggtcatcgat tctcccgttg tcagaaggcc
    12361 gacctgtgca ggccgccgtc gacctcgatc cagcttccgg tgatgtaacc ggcatgatcc
    12421 gaacacagat acgcgacgag cgaggctatt tcgtctgcct tgcccatccg tgccgcggga
    12481 acgcgcgcct cgccgagcat gaattcgcgc cgcgcctctt ccgtccctgc gccaaggttc
    12541 ttctcgaggt acgcgacgga attcgcggtc tcgatccagc ccggcgccac ggtgttcacg
    12601 gtgatgccat agcgggcgta ctcgtcggac accgtcttga ggagtccgat ggtcgacggc
    12661 ctgctggtgt tggccaccac gtgatgaatg tttccggccg gctccttggc cgtggccgaa
    12721 ccgatgtgta cgaagcggcc ccaccccgcg tcccgcatcg cggggagaac ggcgcgcagc
    12781 aagaagatct ggctcatcgt gtgagactcg aaggattcgc gataggtgtc cagatcgtcg
    12841 atgtcggcga agtcgccggg ccgctgatat ttcgcctgac cgaccacgat caagggcggt
    12901 ccgatctgcg tgcgcatctc ggcaagtgcg ttctcgagcg cttcctgttg gccgacgtct
    12961 gcgcatacac ccatcgcgtc cccgccggct ccgcggatgg tgtcgacggc tgcatcgaca
    13021 tcggccttcg tccgtgcaag caccgccacc cggcagcctt cggcggcaag cattttggcg
    13081 acttcgaacc cgatgccctt actgccgccg acgaccagcg ccgacgtgcc agagattccg
    13141 tagtccacga atcggcctcc ggcgctagcg ccgtacgtcc atccccgcgt cgaccttgag
    13201 gtggctgccg gtgaaggacc gcccggcgtc ggagatgagg aacaggatgg cattgctgac
    13261 atccctgggt tcgatcagcg gcagatccgg cagcgctgtc tgcatcgcgt tggccaagtg
    13321 cggcatctct tcgatcacct tgaacagggc cgggttctcg gtgatcatcg gcgtggcgca
    13381 gttggtcggg accaccgcgt tcacccggat gcgatccttg gcaagcagac tcgcgtacgc
    13441 acgcaccagg ccaaggaccg cgaccttcgc catgaggtag gcgtccgaac caccgcgacc
    13501 gtcggtcatg tccaataacg cgatcatgga gctggtcgcg atcagattcc cgccgccttg
    13561 ctccctcaag tgtgggaccg cgatctggaa ggcgttccac acaccgatct gcatgatgtc
    13621 gataccgatg cgcagtgcct cttcagcatc gcgctcgtcg gcgttggtga ggatgacgcc
    13681 cgcgttggcg atgaccgtgt cgatatggcc gaactgctcg acgcccccgt cgaacgcttc
    13741 ctgaagcgcg gccttgtcac gcacgtccgc cttccggaaa agcatttcct gaccggtctt
    13801 ttcgacaagg cgagcggtct cttcgaggtc ttcgtgggtg cccagcttgt agggcacaac
    13861 gtcgaggtct tcacagatgt cgattccgac gatgtttgcg ccctgctcgg cacacgtcac
    13921 ggcgtgcgac cggccctggc cgcgcgcagc cccggtgatg aacacgacgc gcccgtcgag
    13981 tgaacccatt ggtgcattcc tccggtgatg atcggcagct gaccgcgctg gcgcgcggcg
    14041 ctcaggatgg aggcgcgtgt gaagtgcgcc acgacggatg ttacgccgcc ggtttggaat
    14101 catacaacta tttggctaat ttgaatcgac aggctgatcc atgctccgga agggaatcca
    14161 catgtccgac accctcgaag gcggtccccg cccacccgac ggcgactggc tcggaagccc
    14221 ctacctgagg ttcgaacgcg aaggggcgtt cgcgatctgc accttggacc gtccagcggc
    14281 ccgcaacgcg atgacaccgg cgatgtattt cggcattcgc tatgcgatca accgggtaaa
    14341 cgccgatcca gagctcgcgg gtctgctcat cacgggtacg ggtgacgtct tcgctcccgg
    14401 tggcgacctg ggtcagcgcg gtgaagacaa ctggatggac ttcgccgcgg tgatgggtat
    14461 ggacgtgacg ccgttcgacg tcctgcgaca gtcggccaag ccggtggtcg cggccgtcaa
    14521 cggtctctgc cagggcggtg gtctgcagat cgccatgtgc agtgacctcg ccgtcgtcag
    14581 tgaacgagcc acgttccgcg tccctgagct gttccgggga atcgccgaca cctattacag
    14641 ccagatgctg gcgcgcatga tcgggcccgt ccgtacccgc gacctgatgt tcaccggccg
    14701 cacgttgtcg gctgccgagg cgctggactg gggcatggtg gcacgcgtcg ttccccacga
    14761 cgagttgttc gatgtttcgc gggagctcct cgcgcagtgc tgccgaaccg cgccggccgc
    14821 acggggagtg atcaaggcca gcttggacaa ctacatgggt ctctacgatc ggatcggtat
    14881 gcagacgagc ttgttcgggc ccgagacggg cgaagggttc gtcgcgttca aggagaagcg
    14941 gtcgccgtcg tgggtccatc cagacctgcg agtcgacggc cgcctttagc ttgtgagccg
    15001 ccgcggtctg gcctgcgggg gtgtcgacca gaccgcggcg gttgtctcgg tgcctatttc
    15061 ggcgtgaagc gctgtgcacc gtcgaggcgc atgacctcac cgttgacgta accgttgctg
    15121 agtaggaagg cggccgcatc ggcgtattcg gacggcgcac caagccgctt cgggaacggg
    15181 atgttggcgg cgaacttcgc gatggcttcc tcgccgaccg actccatgat cggcgtcttc
    15241 atggtgccgg gcgcgatggt gttgacgcgg atccctaccg agctgaggtc acgggcggcc
    15301 gcgatggtca aaccgatgac accggctttg gctgcggcgt aagcggtctg gccgatctga
    15361 ccttcgtagc ccgcgaccga ggcggtcatc acgatggcac cccgttcacc gtcctcgcgc
    15421 ggatccgcgg tggccaccga cgccgccacc agacgagtca tgttgtaggt gccgttcagg
    15481 tagaggtcga tggtcttgag gaagccgccc atgtcggccg ggctgccgtc gcgctgcacg
    15541 atccgttgtg cgacaccgaa accgccatgc gccacgaccg cgtagcgcag ctgaccgagc
    15601 tgctgggcct ggtcgatggc gccgagaacg ctgtcgtcgc ttgtcacgtc cgtgctgacg
    15661 aaaatcgcct tgctgccgag ctcgtcggcc agtgccttgc ctttgtcggc ggccagatcg
    15721 gcgatcacca cacccagacc atcggcgtga agccgacgca ccgtcgcctc gcccaggccc
    15781 ccggcgccgc cgctgacaat tgccgacgca ccttcgaatt gctgtaccgt caccggttct
    15841 cccttatcca ttgttgtttt ccagatgagt aacatcgccg gtcaccagat gccacggatc
    15901 gtcggcggcc agcgccaagc gccccagccg gcgtgcgaag tgtgcggtag tgccgtagtc
    15961 cccggcccaa ctctgggcac gcaaggtgaa cagccagagc gggtgctcgc tggtgacccc
    16021 gatggcgccg tgcagctgat gagcggtggt ggtaacagga ccaacggctc gcccgaccgc
    16081 gatcttggcg acggtgacgg cgtagtcggt gcgtggcgag tcgaagccgt gttcggcggc
    16141 tgcggccacg gccagttcga ctgcggcgcg tgcccgttcg atgtcgccgg ccatacccgc
    16201 gagggactgc tggacggatt gaaatgcgct gagcggacgg ccgaactgga cgcgcccgcg
    16261 ggtgtgcgcc acagtcagcg ccgcggcggc atcaagggcg ccgatgatct gtatgcaccg
    16321 cgcccacgca ccgcgtcgcg caagctgggc ctccaacgcc gggtccacgg agtgcaactc
    16381 ccgggtcggc acgtcgaacg caatagcatc gcgcggctcg ccggccaggt tgtggttttc
    16441 ttgccggtcg cccgttgccg cgtcgacaac tccgacccgt agcccgtcag caccgcggac
    16501 ggcgagcacc gtgctgcacg cacgcgccca cggcacgccc tgcgcggttc cgacgactcg
    16561 ctcgtccgtg gtttccgcct cagcgattgc gacggtcagc ggtccgtcgg gaagctcgat
    16621 gccggcctcc tgcccgagcc agccggcgag cgcgtcggtt tcggcgaggg gcaccgcgcc
    16681 ggcgtggcgt gccacaccgt agaggacgat ggccagctcg tgcggccccg cgcccatgtc
    16741 gggggtgctc gtcaatcggg tgaggccggt ctcgtcgagg tggcgccaca actcagcgtc
    16801 gaagtggtcg ggcacgccgc ggcggcccag ccttgcgtcg tacgagcgct gtccgagttc
    16861 gtcgacaaga gaacgcaatt cggcatcatc gtcttgagcc gcactcgcag cgaagacacc
    16921 accggctagc gcggtcatct gggccctccg ctcttcgcgc aagcgctcat cgcaacccca
    16981 ttccgcgagc taccactccc cgcaacacct cattcgtgcc gccgcgcagg gtgaacatcg
    17041 gcttgtgcag ccaggccgta gtgagcatcg ccgacagctc cgggttcggc gtcgacccct
    17101 cgagcaggtc ggcgatcaac tcgaccgact ccgcttcgaa gcgggtccct aggtctttca
    17161 ccagcgccgc gaggttcgcc gcgtcgccgc cgtcggtgag tgtgcgagcc accgacagcg
    17221 acagctggcg caaggagatg agccgcgcaa gcagatcgcc gacggcggcg gcggtctggt
    17281 cgtcgggggc gggaccttcg ccgagcattc ggatcgccga cagcagcaga gggccggtgg
    17341 acaggatgcg ctcggggccg ctgcgctcga aggccaattc ggcggtgacc tggtgccagc
    17401 cgttgccgac ctcgccgagc acgtcaccat cgggcacgaa gacctcgtcg aagatgacct
    17461 cgttgaagtg gtgcgccccc gacatcaaca ggatgggttc aacgcgcacg ccctcggcat
    17521 cgcatggcac caggaactgg ctgaacccgt cgtgtcggtg ctcgggatcc ggtgggctgg
    17581 tgcgcgccag caccaccacc tgatgagcgt agtgcgctcc gctggtccat accttcgtgc
    17641 cggacagcac ccaaccgcct tcggcccgaa ccgctttcgt cgaaacggcg gcgagatcag
    17701 atccggcgcc gtgctcgctc atgccgatcg aggagtacag cttgcccgca acgatcttcg
    17761 gcagcagcct tgcgcgctgc tcttcggatc cgtatgacag cagactgggc gccacctgac
    17821 ggtcggcggt ccagtgcgcc gccacgggtg ctcccgcggc gatcagctcc tctgtcacga
    17881 cgtagcggtg caggtgcccc agctcgcggc cgccgtagcg ctgcgggatc gtcaggccca
    17941 ggaaacccgc cgctcccaag cgggccgaga acccctcgtc ccactggccc aaccacgcgt
    18001 cgaccgcggg tttccaggcg aactcggcgc ggtcggcctg taggaaatct cgcaccgagg
    18061 cccgcagctt caccagttcg ggaccgttgg cgctcaactc gtcaaatgaa ctcaccggag
    18121 gagactccta caccgcgggc aacggcgtcg acggcaggta gaagttcggg gtcaggtcgt
    18181 cgtcggtggc ggccagccgg taacccgaca ggtcggtgac accctctgcg cgcaacacct
    18241 cttcatcggt gtagaagtgg ccgctgcagt cagcggccgg gcgggtcacc agagcgtgca
    18301 gcgcgtcggc catgatgcgc gggtcgcggg cctgggcgcg caccacgtcc tcacccatcg
    18361 ccaccatcat tccggtgctc gcgacggtgg tagcaggcca cagcgaattg acggcgatcg
    18421 ggatcgacgc gaattcagcg gcccatccca acgtcagcag actttcggcg tacttgccga
    18481 ccgtgtgtcc cacgtgcgcg ccaacccacc gcgggtccag attcagcggt ggtgacacgt
    18541 tgacgatgtg ggggttgtcg gagttccgca gatacgtgaa cgccgcctgc accaccgcga
    18601 acggaccttg gacgttgatg gcgagcaggc ggtcgaagct cttcggcggc agtttcgggg
    18661 ttggtcgcag gtccagggcg cccgcattgt tgaccacgac atcgatgccg ccgaactcct
    18721 cggcagcagc cgcgatcgca ttcgccaccc cgtcggcgtc gcggacatcg actgcgaatg
    18781 ggaaggcatg acctccagca tcgcggaccg cctcggcggt ctcggtcagg gtgcccgcga
    18841 tcttcgggtt cggcgtttca gtcttggcca gcaacaccac cgaggcgccg tcggacgccc
    18901 cccgaaccgc gatttcgcgg ccgatgccgc ggctggcacc ggtgacgacg acgacacgat
    18961 ccttcagggt gcgaggaccg agaattccca cgacgccgtc ctctccctgg cttagatgcc
    19021 gagctactgg aatgctataa ctatatagct cattaacgta ggcggagaat cgcgataccc
    19081 cggtggagga gaagcacacc atgacgcacg tcgtctcgat cggcacctac ctgccgccgt
    19141 ggacggtgcg cgagcgacgg gtcaagggac cggacgagga tgcgctgacg atggcggtcg
    19201 ccgccggccg ggccgccgat cccgacgcga ctgcagcacg cgtcgtgctg gtgtcgcgcg
    19261 acttcccgct gctggaaggc ggcaacggcg ccgtgctgct ggccgcgctg tcgctgcctg
    19321 cggacacccc ggtgaccgag gtgctgggcg gtgcgcccgc cgcactcgac caactcgtga
    19381 gcgcttcacc gggcaccctt gtcatcggtg cggacgacaa caatcttgcg gcgggcgcgg
    19441 cggccgcgct caccggtgga gacggcgcga ccgtaaccgg cgtcgcccgc cagacccgga
    19501 gcctgccgtt gaccgcccgt ggcgaggacg gcacccggca cgcctacgtc gacccgcgac
    19561 tgcagcgcga ggtcggtgtg cgctcaacgc tcgcccggct tggcctgacc ggaacgccca
    19621 cagtcgccgc ggtggcgggc gcgccgccgg gtcagctcgg taccggcttc gacacctccg
    19681 acgccgcggg cgacgcgacg ccctccgccg gtgccatcat cggcctgctg gccgaggcga
    19741 tcgaggcagg tgcacaggga ctgctgctcg cggtcgaaca ggccagcgtc agcgcggccc
    19801 agctcacgct gggagcagcg aagccacgga tatcccgcga cgaattctcg ccacaggagc
    19861 tgcctaagac ccgcgtggca gacggcgtcg ggatcccgat cagcatgccc gcctacgctc
    19921 gagcattcga gccgaaaata cgttgggaag cagcggtttt cgaggagagc cccggtatcg
    19981 actccgcccc gcagtttccg ccgcggttgc gcgtgaacaa cgccggggag ctggtggaca
    20041 cctaccggct cgaagcactg ccgcgtaccg gcacggtcta cacccacacc acggtgcgga
    20101 ttccggtgcc cgacctgccc agcccctact cgctggccgt cgtccagctc gacgacagcc
    20161 cggttcgcgt gttgctcaaa gtcaccggcg tgccccccgg cgagacgacg atcggtcagc
    20221 acggtgccgt ggtgctacgc aagatcgcca cccgcgcagg cgtccccgat tacgggtatg
    20281 cgttctggcc ggggcgcaca cttagtcccg aaggagtcaa cgcatgagga cccgaaggcg
    20341 atcgcgagtg aggatcgcag cgcacaacga gcgaggaccg gagcgagcgg gagccgaggc
    20401 atgaggaacg tcgccatcgt cggcgccggg atgacgccct tcgccgaaca cttcgagctc
    20461 ggtgtcaagg atctggtgcc gatggcgtac gcagaagcgg tagccggtgt cgacaagggc
    20521 atcgacaagt ctgaaatcca ggcagcatgg ttcggcgagc tgtccaccac cgacggcttt
    20581 ccttctggca ttctggcaga cacccttgac ctcaccgaca tcccggtcac ccgtgtcgag
    20641 aatgcttgtg ccacaggcaa tgacgcaatc cgcaatggga ccatggctat cgcctcaggg
    20701 ctctatgacg tggtgctggt ggtgggtgcc gacaaagtcc gcgaaacgtc gtcgaacacc
    20761 acgttctggg actgggcggc gatgacacgc gacaacgcat gggactaccc gctcggactg
    20821 gtggcgcccg ccaacttcgc gctgcacgtc atcaggtacc tgcacgagtc gcctgccacg
    20881 aaggaacaca tggcgatggt ggcggtgaag aaccacttcc atgcgctgaa gaacccgaaa
    20941 gcccaactgc gctacgagat caccgtcgag caggctctgg cggcgccgat cgtggtggag
    21001 cccttcggcc tgtacgactg caccccgcag agcgacggcg ccgccgcagt gatcctggcg
    21061 gctgaggagg tcgtggaccg ctacaccgat cgcccggtct gggtgcgtgg tgtgggactt
    21121 ggcatggatc gcgtgatgca ccagcacaag agcgacatga cgacgttccc gccgacggtg
    21181 cgcgcggcca agtccgcgat gacgatggcg ggcgtcacac ctcgcgacat cgacgtcgcc
    21241 gaggtgcacg actgcttcac cggtgtggaa ctcatcagct acgaagacct cggcttcgcc
    21301 aaccggttcg aggcctacaa gcttgtcgag ggtcgcgagc actacgtcgg gggctcgatc
    21361 cccgtcaacc ccagcggtgg tctgaaggcc aagggtcacc cgcccggtgc aacgggggtg
    21421 gcccagtgct acgaactgtt caaccaattg cgcggccagg ccgagaacca agtagacggc
    21481 gcccggatcg cgttggccca caacatcggt ggtccgaccg ctgtctcggc ggtcaccatc
    21541 ctctcgaccg agaagaactg acaggagtcc catgaccacc tcacaagtcg cgacgctggc
    21601 cggctatgag cagttcgcgc catggctatt ggtcgaaaag cgcgacaacg tccacgtggt
    21661 gagcatcaac cgccccgagg cgctcaacgc tgtcaccgaa gaggtgcatc acgccttcgc
    21721 caccatctgg aaggtgctgg atgcagataa tgacgtgaaa gcagtcgtga ctaccggtgt
    21781 gggcaaggcg ttttcggccg gcggtgacat ggtgatgttc ggcaggctga tcgaggacga
    21841 ggtggcccgc accttccaga tccacgaggc ccggactgtt ttcctcgagg tgatcaattt
    21901 cccgaagccg ctggtgtccg cggtcaacgg ccccgcagtt ggattgggct gttcgattgc
    21961 cctgctttcc gacctgctgg tgatggggga gagcagctac ctggctgacc cgcatgtcgc
    22021 ggtcggactg accgcgggtg acggtggtgc tgccatgctg cctctcctga tcggcatgat
    22081 gaaggcgaaa gaatatgtgt tgcttggcga tcgaatcacc gcacccatcg cggagaagct
    22141 gaacctggtc accaaggtgg tcagtgacga caccgttctc gacgaggcgc tggctctcgg
    22201 cgaacggctg gcggcccttc cgcctcaggc gttgcggtca tcgaaggtgg cgctcaacat
    22261 gcacctggcc cgcgccgcac agggtgtgct cgagtatgcg ctcgccgaag agctgacgtc
    22321 gttttcgacg ccggagttca aggagcgggt cgcagccttc cgtgcccgtt ccaagaagta
    22381 gggcggtcat ggtcacgatg aagatgcggg cagccatcgt tgtcacgggc atgatcgcag
    22441 cggcgttcgg cgcgaccgcg cccgcgggcg ccgatcccac ggacgatcct tgccaactcg
    22501 ccgtcacttt tctgtgtcga ttccttccga tggcacccga cctggaccac aacgtcgacc
    22561 tgacgaagga acccgccatc gtcgacggcc aaccgcttcc cgaaatgcct gctgggacac
    22621 aatcgcagga cacatcaccg gctgacattt gcgtcgacgg ctgtgcctag tcggctttga
    22681 aaccggtgct gaaagcaccc ttgcccgtgc gacgaagtgg gctgctagcg atacgccgtg
    22741 atgcgggcgc taagcgcaat cttgccgtcg tcgccgatgg ccactgcgtc cgcgattccg
    22801 gtgctgcggc cgactcgcac cggcgtcccg acgtagcggg aggcggtgcc cgcgaggaac
    22861 gggcgctgga agttgacccg cagcgatgca gtcttgagaa agccggcgga cgggtcgggg
    22921 ttgatcgccg ccgacgccgc caattccagg gccgccgagg ccatcccgcc gtggacgatg
    22981 ccgatgtcgt tgttgaggtc cggatcggcg tgctgcttca acacccgtga atcgtctccg
    23041 gtcggttcgg cttgtgccgc catgagatcc gccaaggtgg gtgtcccgcc cagcagcgtc
    23101 tgcggcggac gcttatcggt gacgccgtcg gcaggaatga agaacgagcg cacagttccg
    23161 gtgccgatca cagtggcgcc acaggtcagt gtgcagatcg acagtgccgt cgtcccaacg
    23221 gaacccactg cgcctgcgct ggccaccacg ggctcggcaa ggtcatcgag cgcctcggcg
    23281 acgttggggc tgacctcaag cgccagctcg ctggacaccg tccactgatc ggtacggcgc
    23341 cggtagtgat tgaccatgcc agcggccgcg tcgacgagga tggccagcgg cccgacggcc
    23401 ggcgccccgg tgaacggatt gcgcataccc gacatcggca tcgacatctt taccgtcgcg
    23461 tcggccaggc ttgccgcgag aaactcgatc ccgaaccgaa tctgtatcgc ggccggagtt
    23521 tcgatgtcct gctgaacttc tcgctcgtct actccggtca ttcgagaagg tctaccaccg
    23581 ttgacaattc ggcccccgac agtacgaagg cctcggcctt ctgaagatgc ttcttccaca
    23641 gttcgccggc ctgctcggcc tcgcctgcct tgatcaagtc gagcaccatg cggtgggtct
    23701 tcaccgagcg ggttaccgcc ccggccgcgc gcgtgccttc ggtcggttgc aacgatcggt
    23761 tggctttctc gatgatgtgg tggagcatgt cgctgacgat ctgcagggtc cggttacccg
    23821 acaaccttgc gattgcggcg tgaaaatcgg tcaactggtc gacggtgtca cccccgggtg
    23881 acaattggtc ctcgcgttcg acgatcgcct cgagttcggc gatcaccccc gggttccggt
    23941 cctttgcgag ttggacgacc atcggggtct ccagggtcac cctggcgtcg tagacgtctt
    24001 tgagcgtcac accctcatac tcgaggatga gtcccgcata gcgggccagg gtcttgcgcc
    24061 gcggccgggt aacgcgggca ccgccgcgaa caccgcgctg gacctcgatc agcgactctg
    24121 actccagcac ccggaatgct tcgcgcagcg tcggccggga caccccgaaa cgctccatga
    24181 gttcggattc gggcggcagc atggtgcctt cggtgatctc accgcggatg aacatccggc
    24241 gaagcacggt ggccacacga tcggccatct tcggttcgcg aagactggtg acctccatat
    24301 acccgacctc caccacttaa tgttgcaaca attaactaat tatttaaggt tatccgaatc
    24361 cctgacccga gcctggcatg cgcgccggtc agtgactaat gtcgccgccg gcgtggctca
    24421 gtgaccaata tcgccgccgg gagcggctca gtgaccaagc ccaccgtcaa taccccatcg
    24481 ctcggcccat caggtccttc atgatctcgg tggtgcccgc gtacaaccgc tggacgcggg
    24541 agtcccgcca cagccgggcg atctcgtatt cgttgatgta gccgtagccg ccatgcagct
    24601 gcaggcagcg gtcgatgatc tcccactgca cctcggaggt ccaccatttc agccccgcgg
    24661 catcctggtc cgacaacgtg ccgtcgttga ccgagagcac gcactggtcc agataactct
    24721 gcgcggcgtc cagtttggtt tgcatctcgg ccagaaagtg ccggttcacc tggaatttgc
    24781 cgatcggctg tccgaacgct gtccgctcga gcgcataggc cttcgtcagg tccagcgccc
    24841 gccgtgctgc gggaagcgcg tagcaggcaa cgccgacgcg ctcgctgggg aggttgccca
    24901 cgaggtgata aaaaccgcgg ttgagctcgc cgacgaggtt ctccttgggc accacgacgt
    24961 cttcgaagaa cagttcggcg gtgtcggcgg agtactggcc gatcttgtcg agcttgcggc
    25021 cgcgggtgaa gccctccatg ccgtcttcga tcatcagcag gctgatgccc ttgtgccggg
    25081 ccgccgggtc ggtcttgacg gcggtgagca ccaacttcga gagcagcccg ttgctgatga
    25141 aggtcttctg gccgttcacc acccagtggt cgccctcgtc gcgagcggtg gtcttgatgc
    25201 ccgccagatc ggaaccggct gcgggctcgg acatcgcgat ggagccgatg tactcgccgg
    25261 caacgaattt gggaagccag cgctccttct gctcgtcagt ggtgaggttg ttcatgtacg
    25321 gcatcaagat gtcgttctgg acaccgagac cgatgccgac cgatccggtc aagaacatct
    25381 cctcggagat gatctgattg aaccggaagt ccttgacccc gaggccgccg tatttctcgt
    25441 cgaactccca gccgatcagc ccgtgttcgc ccgcggccag ccaggcctcg cggctcacct
    25501 tgccgtcgcg ctcccacttc tcgacattcg gcacgcattc gcgttcgaag aagcttctgg
    25561 cggtggcgcg gaaatcgtcg tggatttcct cgaatatgtt gcggcgcata cgtcccccta
    25621 attccagttg accatcgccg gtagcgcgcg cccgggcatg taggtgtcga acagacgctc
    25681 ggcaagcagc gattccagct gtagatacga gccgcacagc gcgtcgatct gaaatccacg
    25741 cgccacgtag cgatgcagat cgtgttcggc ggtgagtccg atcgcgccgc acacctgcag
    25801 caccgcgtca ctgactgttc gatgggcccg gcccgcagcg attttcgcgg tctgcgccga
    25861 caaaatcccg ccgtagcacc atgattcatt gagcagtgcg cgcgcgccct cgagttgagc
    25921 cgcggcgtcg gcaagtgcgt ggcgaggcga ctgcagcgat ccgatgggcg atccgaactg
    25981 gacccgaacg ctgatgtgct caacggcgat ccgcagcgcc tggtcggcga gcccgatgag
    26041 ttcggtggct atcgaccgat gcgccgccgc gatcgcgcgt ccccattcgg tcgaggcgtc
    26101 gatcagcgcg tcatcggtcg tgccctctac gcgggtccag acgacggacc gatcgaaggt
    26161 gtccatcggt tcgccgcgta gttcatcggc atcgacgacg gctatggaaa cagttcccat
    26221 cgctcgcgag accggcacga cgacgcggcc gcgaagaggg ccgagcacaa tgcctttgac
    26281 ctgatattca ctcgaactag gcgcatagcc gtgggacagt cccggcagca cgatggtgtc
    26341 ggccggtgcg tcgagtaacg acgccagttc ggccagcatg acccgatcca acgcgtcggt
    26401 gagggccagc gaatgacctt gcgcccgaaa cagcaacgcg catgcttcaa ccgggtattc
    26461 agactcgatc tcggcccagc ccatttccgt gagccgcgga ccgactacgg tgtgttcctg
    26521 tttggcggcc agttcttcga agagccggaa gaccgcctcc tcgaccatcg gccaggcttc
    26581 gtcttgcgga ttcgctcctc gcgtgcgcat cacgatgcgc gcctctcctt cggaagcccg
    26641 aggatgtggt cggcgacgat cccgcgctgc acttctgcag tgccgcccat gatggtcgcg
    26701 gcgcgtgagt accaccactc cgcacgtgcc gtgtccagtt cggcctctcg attgacgccc
    26761 ggtgggctgc ccgccagcag ataatctcgt gcgacgtcca ggatgaggtc gttgacctct
    26821 ctctcggccc tgctgcacag cagcttgtct atgctgcttt ccggacccac aggttcgcct
    26881 gccgcgagtt tgcgcaccgt cgtcgcggca cgggcttggg cggctatcac gtcgacgtag
    26941 acgcgggcga accgctcacg ctgcgcggcg gttgcgttgc tggtgaccat gtactccctg
    27001 agccgtccca actcggtgag caccttgttc aagacggcat agccgtacat tccccgctcg
    27061 tactgcatga ggaacatcgt cacggcccag cctttgtcga cgtcgccgac cagccgctcg
    27121 cggggcacgc gtacgtcgtc gaaatacatc tcggcaagct cgcgtcggcc gctggccaat
    27181 gcgatcggcc ggatggtcag gcctggggta tcggtgtcga tcaacaacat cgtcaaaccc
    27241 cggtggcggc tttccggcga cccggtacgc gccagcacac agactcgttt ggccgtcccg
    27301 ccctggctgg tccagatctt ctggccgttg acgacgaagc cgccggcacc gtcgtcggcc
    27361 gctcgcgtac gcagggacgc gaggtcgctg ccggactctg gctccgagaa gccctgcccc
    27421 caccattccg agccatcgag gtacccgggt aggtactccg cagccagctg cggtgcgaat
    27481 tcgagcacgg ccgggcccat cacttccagc gtccacgcct gggctgggat gggcagcatg
    27541 gcgtggccga gttcctcgaa gtagacggcc ctgtgcacct cattgccgcc gaagccgcct
    27601 gccgcctcgg gccacccgta ccgattccag ccgtccgcat gcagccgtgc catcacagag
    27661 gcgtcgtgct cgaggccctg ctcggctgtc atactcgcag ccgatcgcca ctgctccgcg
    27721 cagtccagcg tgcgcaggta ctcccggaga tcctggcggt actctgcgac ctgcgacagg
    27781 taggcgtctc ggcggccgcc gctgacgccg ttgttgacag ggaacgacac cgctacctct
    27841 tcgctactcc tggaccctgc ccctgggggg tgaatccgct aaacaattat ctataacggg
    27901 tacactaacg cacgacttca acttcgccat agggagcgcc aatgacggca tcaccggtcg
    27961 cagcaacaac ggtccagttc aacccggaaa gccggctcta tatcgacgga gaactgcgcg
    28021 aagcgtcgac cggcaagacc gccgacaaca tcaacccggc caccgaggaa gtgctcgggg
    28081 tgaccgccga tgcggatgcc gtggacatcg acgaggccat cgccgccgcg cggcgcgcct
    28141 tcgacaccac cgactggtcg acgaaccacg agttccgtca gcgctgcctc atgcagttgc
    28201 acgaggcgct gcaggaggag aaagaggacg cacgcgcgga gctggttgcc gaggcaggcg
    28261 ccccgctggg catgacctac atcgcacagc tggactggcc gctggccgac gccgtccgct
    28321 ggcctgcaca gcacatctcg gagttcgcct gggagcggat gctcgacgtg gacgccaaga
    28381 tgggcgagcc ctacaaccgc gtggtggtca aggagccgat gggcgtcgtc ggcgcgatca
    28441 cgccatggaa tttcccggtc gagatcatct ccaacaaggt gggccaggtc ctggccaccg
    28501 gcaacacaat ggtgctcaag ccggccatcg agacgccgtg gagcgcgctg cgctggggtc
    28561 gcatcatcgc cgagaagacc gacattccag cgggtgtcgt gaacatcgtc accgcgtccg
    28621 acaacgacgt ggcccagcgg ctggtcaccg atccgcgggt ggacatgatc tccttcaccg
    28681 gctcgaccgc cgtaggtgaa ctgatcggcc gcctgtccgc cgcgaccatg aagcgcaacc
    28741 tgatggagtt gggcggcaag tccgtatacc tgctactcga ggacgccgac ctggccacga
    28801 ccgtccccgg gtgcatcggc gcactgatgc attcgggtca gggctgcgcg ctgaccacgc
    28861 gaatgttggt gccgcgcgat cggtatgacg aggcggttga gctggctacg gccaccttcg
    28921 cgtcggtcac cgtcggcgat ccgaccgacc ccgccaactt ctgcggccca ctcgtctcgg
    28981 ccaagcagcg cgaccgggtg ctgggctaca tagaggtggc caaggaacag ggcgcccgtg
    29041 tgacggttgg cggcggaaag ccggagggac tcgaccgcgg gtacttcgtt gcgccaacgg
    29101 tcctggccga cgtcgcaccc gaccatcgcg tcttccagga ggagatcttc ggtccggtca
    29161 tcacgatcac gccctacgac ggcggtgacg acggtgcggt cgagatggcc aacaactcga
    29221 tctacgggtt ggcgggcacc atcctgggtt ccaaccagcg cgcgatggcc gtggcgcgcc
    29281 gcatccgtac cgggtcggtc tgcgtcaaca gcggcatctt ctacggcgcc gatgcaccct
    29341 tcggcggcta caagatgagc gggctcggca ggcagaacgg agtcgagggc ttcgaacagc
    29401 acctgcagac caaggtcatc gcctaccccc tggagacggc gtgagcgaca tcctgagcgg
    29461 tatccgcgtt gtcgaactcg cggcgtggac gttcgtcccg gcggcggggg ccgtgctggc
    29521 cgactggggc gccgacgtca tcaaggtcga gcaccccgaa acgggtgatc cgcagcgggg
    29581 gctcatcagt tccggactcg tcaccggcgc agggggagtc aaccacttca tcgagcaacc
    29641 caaccgcggc aagcgcagca tcggactcga cacgtccact cccgaaggac tcgagctctt
    29701 gatgaagctg atcgagaccg ccgacgtgtt cgtcaccaac ctgctgccgg attcccgtca
    29761 gcggatgggc attgacgtcg agcaggtccg ggcgcgcaac ccgaagatca tctacgccag
    29821 ggggcacggc tatggcacca ggggcgacct ggcatctcaa ggtggtttcg acctcgcggc
    29881 gtactgggcg cgcggcggca tcggcgaagg gtatgccgca ggcgacggtt cctatccgcc
    29941 gatccagcgg ccggccttcg gcgactccta cggcgggctg gccatcgcag gtggaatcgc
    30001 cgcggcactg gtgaagcggg aacgcaccgg cgagccgtcg gtggtggacg tctccctgct
    30061 caacgcggcc atctggcagt tgggccccga catcgtcggc tcgggtgtca cgggccagga
    30121 catcccgaag ttcaacctcg acgagatgcc caaccccgcc gccagcatct acaaaacccg
    30181 cgacagccgg ttcattgcat tcgtgctgct gcaggccgac cggttctggg ccgacttctg
    30241 cacgcgcgtg ggacggcccg acctcatcga cgacgagcgg ttcgccaacg cggcggtgcg
    30301 gttcgagaac cgcaaggatt gcattgccga gctgcggaaa gcttttgccg cagaggatct
    30361 ctcgcattgg gagaaggcat tcgccggatt cgacggggtt tgggacgtca tgcggaccgc
    30421 ccacgagctg cacgccgatc ctcaggtgat caagaacggc tatctgcctc gggtgacgaa
    30481 tgccgacaac aacgagttcg cgcttgccgc cagccccgtg cagttcgacg agacaccgct
    30541 ggacctgacc tgcgctcccg gccatggcga acacaccgac gcgctgctgg ccgaactggg
    30601 gtacaacgag gacgagatca tcgatttcaa aatcaagtcg gttgtgctgt agtggttttg
    30661 gagcagctgg cccggcggtt cctgcacgtc aatctgaact gcgactcgct cgatgcgacc
    30721 gagggactgt acgtggacaa actcgggctc tccgcgagga tgcgaacgga ccccgaggtg
    30781 gctacagacg gctccatcct cgggcttgac ggtgaaacct tctgcgctac atcgtttctc
    30841 tatgattcgc gcggtggccg gggcggctgt gcgctggagg cgatcgagta tcgcagcccg
    30901 acgctgaagc gcgacccaag cagtgatccg gcacgccccg gcatcagatc gacattgatg
    30961 accgtagagg acgtggggga cacggtaagc aagctgcgcg aagcgggtgt caccgtcagc
    31021 gatcccgttg acggtctgat ctccggcacg aaatcggtgc tggcgtccga ccccgacggc
    31081 gcggtgatcg aactcgccga gagtgcgcct gatgtcaaag cgtccgacgg tgcgctgttc
    31141 ggcggcatac gcatttcggc gatcaatgcc gtggccaccg gggagttcct gaccgcaatc
    31201 ggattcgccg aggtggaagc cccgaccctg gtcaaagtcg ccggtgatca gatggacccc
    31261 ggtggatcgt ccgatgcggt ggaatgtgtg gtgtcgcgat acgccgtacc ggaggacgcc
    31321 caccagttca gcctcgtcgt cgtgcagcat cccgagaccc gaagccagga tccggtgccg
    31381 tggggcggca atcatcaggg gctgtaccga tgtgcgatgc gggtcgacag cgctgagcaa
    31441 gcgctcgccg ccgtaccgga ttccgttgag gtgatgggag atccggtctg gtgcccactg
    31501 ccgggcacga agatcgaggg cctctacatt gcgttcgtgc gttcacccga cggagtggtt
    31561 ttcgagttcg tcgaacgccc gctggcacac ttcaccggct agcaggcgga cccggatgca
    31621 acagttatcg ggcaaggtgg cgctggtcac cggcgcggcg cggggtcagg gacgtagcca
    31681 cgccgtctcg ctggccgacg aaggcgccga catcatcgcg gtcgatatcg ccgcagacat
    31741 cgaatcgatt ccctacgcgt tgggaaccaa agaagatctc gacgagaccg ctcagctgat
    31801 tcggaacacc ggccgtcggg ctgcaaccta tgtcaccgat gtacgccacc tcggtgagct
    31861 gcaggcgtgc gtgcaggcgg gcatcgccga actcggcgac atcgacatcg tcgtggccaa
    31921 tgccggtgtg gttgccaccg gccggaccga cccgttcgac gaacaggtgt atcgggacat
    31981 cgtcgaaacc aatttgttcg gcacgtggaa cacgatcgtg gccgcggtgc cctcgatcat
    32041 ccgtaaagga cgtggtggat cggtcatctt gaccagttcg acacagggat tggtgggacg
    32101 cggtggtgat ggttcggcgg cggcgttcgg ctacgcctcg tccaaacacg gcgtcgtggg
    32161 cctgatgcgg tcggcggcat acgcctacgc ggagcacaac attcgggtca acacggtgca
    32221 tcccaccggg gtggcgactc ccatggtgct caacgaacac atgggccggg tcttccagga
    32281 gaatccgtcg gcaccgaaac ttgccaccaa tctgctgccc gtcccgttca tcgaggcaca
    32341 ggatgtcacg aaccttgtgg tgtggctggc gagcgacaag gcgcgctatg tgaccggcgc
    32401 gacgatgccg gtcgacgccg gttacaccgc gttgtagcgg gcgcaatgac acaagcagca
    32461 gggctgatcg aattcctccg ccacaatcgc cgcgccttcc tcttctgccg cgacgagtcg
    32521 gggcggccga tcggttacgc gatgcaatcc gtcggatgcg atcgcacgag cctgtacttc
    32581 tcgacgtata ccaacagccc gaaagtgaag cacctgcgtg ccgatcctga agctgcctgc
    32641 atcgtgctga gcgggcagga cgtcatggat cagccgtggg tgtcgctgcg cggtagcgcc
    32701 gaggtctatc gcccctccgt cgatgagatc gacgaaatga tcggtgccgg aacgcctgac
    32761 gggcgcgtgc cccacgctgt ggtcgccaag gtgcgcgatc ggctgatcag cggcaagcgc
    32821 tgcttcatcc gattggtgat cgacgaggtg tgtgcgcatg ccacgtgagc gcagacgcgg
    32881 cccggttgcc atgtcggcgg acgaactggc ctcgtttctc gaacagcgac cgacgggggc
    32941 gatctgtgtg accgactccg ccggtcggct ggtggccttg cccgcagccg tccttggcca
    33001 ggaggacgac acgctaaagg tcgaaatcga tggcctcgac gagccgctcg tgccgggcac
    33061 ggctgtgtgc gtcgtcgccg accgttttcc ttcttacgag gcgatacgtg ggatcatcac
    33121 acaaggttcg atcacgtcca ccgagcatgt ccggccgccg tcggttgcgg ttgcggttac
    33181 tcgtacggtc acattttcct tcgccaatgt catcacgtaa gtaccgtggc ctcgtcaacg
    33241 ccgcagctct cgcaatccgt aagcctctgg cttgggcgaa acgtcggtag ggcgaaaggt
    33301 gttggcgact ctgactgatg tgctgaacct gttcgacctg tcgacgctgg cacccgacat
    33361 cttcgagggc aaccaacccg acccgccgaa tcaccacatc gtcggcggtc agattgctgc
    33421 gcaggcactg atggcggcta gccggaccgc cgaggggcgg cctccgcaca gcctgcacgt
    33481 ctacttcctg cgccgcggcg acgcgcgctg gcctgtccgg tttgaggtca cccggctgca
    33541 cgacggcggc aggttctcca cccgcagggt cgccgccgtc caggacggcg cggtgctgat
    33601 ggaggggctc gcgtccttca ccgccggcgt cgaagcagcc gactatcagc gcgcgatgcc
    33661 cgacgcgccg gagccggagt ccttggttcc cgtcgacgag cagctggcgc cctacgccga
    33721 cgaattcggc gactggtggg ggaccgagcg cccattcgac gtccgctacg tcgatccgcc
    33781 gccacgcgtc gccgcggacc gaaaacaacc ggcggggccg gtgagccgga tctggctgca
    33841 cgccaacggc tccgttccgg ccgatccggt agtgaacagc tgcgtgctga cccttctcag
    33901 tgccctcacg ctgttggagg ccgcgctggg gccgatgcgc aagtcgcagc gcgacgtctc
    33961 ggcgctgctc gatcacacgg tgtggttcca tcggccggcg gatttctcgg attggctctt
    34021 ctatgaacag aattcgccaa gcggtatcgc cggacgcgcc ctatcgacgg gcaccatcta
    34081 caaccggcgc ggggatctgg tgtgcacggc cggccaggag gggtatttcc cgcccgccag
    34141 ggtctagggg gcagatcacg ccgtcggcaa gggacctctg aggccgcgcg gggacgtgcc
    34201 gtacagccag ctgaccgcct ggcctatctc ggaaaatcgc agcgacacac cgtctttggc
    34261 atggtagcgg ccagccgcct cggtgaaacc ctcgatgcga ctcagcgtgt ccccgttgac
    34321 gcgcaggatc tggcccgtca accaggaaga gtccggcgac tgcagccacg cgaccacgtc
    34381 cgagctgcga gcggggtcca gcgcggggtc atcggccctc tcgccactga agaagtcctc
    34441 actgatgcgg gtgacggcca gcggcgaaat cgcgttcacc gtaaccccgt agcggcctgc
    34501 ttccatcgcg gtcacgacgg tcaggttggc gatggccgcc ttggcggcac cgtaggcgct
    34561 ctggccgaca ttgccccaca gccccgcgcc cgacacggtg ttcacgatgc gcgcatcgat
    34621 ctgattgccg gccttggctt gtgcgcgcca gtactcgcag gcgtgtttgg tgacggcgaa
    34681 cgtgcccttg aggtgaaccg cgatcaccgc gtcccagtcg tcctcggttg acgtcgccac
    34741 catcgcgtcg cggacgatgc ccgcgttgtt gaccactccg gtgaggctcc cgaacgcctc
    34801 gacggccgtc ccgatcatgt cggcgacatc gtcccaggac gagacggatc ctgtgtgcga
    34861 caccgccttc ccgcccgccg cctcgatctc ggcgaccacg tcggctgcgg ggcccgcccc
    34921 gtcgccggca gtgccgtcgc gactcacacc cggatcgttg accacgaccg aggcaccctg
    34981 agcggacaac gccaggcagt gtccccgccc gatgccgcgt ccgccgccgg tgaccaggac
    35041 gacacgtccg gaaagtggac cagtcattgc actccttact gaaacagcgt ggcgatgagc
    35101 cgacgtcgct gcggccaatc cgccccgcgc aggtccgcgt ggccggattc ggtgacgatc
    35161 aggtcgacat catgggctgg ggtggacacc gggcggctca actggtcgac cagcggcgag
    35221 cgcccgttga ccgtcgtcgg gaccgcgatg atcgaaaggc cgccgtggct cattcggcct
    35281 gccgcgcagt agtcgggatg tccaccgatg cccccgacca ccttttctcc gcggccctcg
    35341 acgttgacct ggccaaacgg gtcgatttcg atggcggtgt tgactgcgac gaacggctcg
    35401 cccctcgaca gtcgcgaaag gtcgtgggtg taatcgatgc cgcgcaggat cggtttgccg
    35461 tctgcccagt cgtagagcac ttccgacccg aagaggtaag tggccgaagg catgtcgacc
    35521 agcattccgc gcttgtcaag gtcgaccacc gcgtcggtga gcatgccggt gtcgatgcgc
    35581 agcggcaccg cggcacgccg tagcagtgcg gttccgagtt ggccgggtcc atactgcagc
    35641 cgcgcatcct ccggaacgaa ccgcaacacc gcgtcggcga gtgcgtcgtg gattggttcg
    35701 gggtccctct gtggcatgcg tgccggtccg tcgctgctgc ggccacacac gtgcacggcc
    35761 gcgggatcca gcggcggacc cgcgtcggcg cacggcgagg tgttatcgac gacggcgagc
    35821 accggcacgc cggcatccac gagtccccgt tgccacgcaa cctcggtgcc gaagtgcaaa
    35881 cgcccggcgc gttcgacgac tcgggtcacg agcacgtcgg ggcgcagcat gccggtcagc
    35941 agtgaaggta ttgcgctgag tcgggtcggt acgaaacgcg ctgtcgggct ggccaatacg
    36001 gtccgcacac cccagcccgg catcaatgcg acggtttcgg cgaaggcgtc ggcttcgata
    36061 ccgtcgagag acgtcggtaa ccaccccagg atcagccgca ccgagccgac atcgcgggcg
    36121 agcgccgaga ttgccgcccc caccgacgtg ccgtcgtcga gacagcgcaa tgctccgacg
    36181 ccgtcgccga tcgcgacggt gccgcctggg cgcactcggg tgcgcaatgc cgcggcgagc
    36241 tgcgtcgaga tcgacgtttt ggcgctcgct actcgcactt ttgcgcccaa gtgttggttt
    36301 gggcgagacg gtggagggct caccgtggct ctttggggag tcccagcacc cgttcaccca
    36361 gaatgttgcg ctgaatctcg ctggtaccgc cgagaatggt ctgggcgcga cccacgagca
    36421 tgtgttggac gagttcgttg tccgccggat cggcacacgc atccgtgccc agcacctcgg
    36481 tggcgagatc gcccaggtcg cggtcggtct ccgaggtcat caacttgagc accgaaaacg
    36541 ccgacgacga cgtgtcgccg gcgtcgatgg cacgttgcca ggtgtaccgc agcagccaca
    36601 tccgcgccca cagcttggtc atcgaccgcg caagtaccgg atccagcaga tcgtgatcgc
    36661 gagccgcgtc gaccatccgt tcgtgaagcc ggaacatccc gaccgcctgc gaaccgagcg
    36721 tcagcctctc gcgtcccagc gtcatgatcg cgacggccca accctgatcg acttcgccga
    36781 tcagtgcgtc gtcttccaac tcggcgtcat cgaagaaaac ctcgttgaac ttgctctcgc
    36841 cgtccatctg cgtcagcggc cggacgctga cacccggcgt gtccatcgga atgacgaaca
    36901 tcgacaggcc gcggtgcttg tcgggcccgg tacgggcgag cagcaaaccg tagtcggcgg
    36961 agttcgcggc ggaactccag actttctgac cgttgatccg ccagccggtc ggcgtccgtt
    37021 ctgccttggt gcggacgccc gcgaggtcgg agccggcgcc cggttcggag aacaactgcg
    37081 tccagatgtc gtccccgagc cgaatgggt
//
LOCUS       DASYLX010001008         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1602789,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001008 DASYLX010000000
VERSION     DASYLX010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602789"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGP27_23805"
     CDS             complement(<1..527)
                     /locus_tag="VGP27_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEV7854356.1"
                     /translation="MTSPEQPDETDIPEQFRIRQAKRERLLAEGREPYPVEVARTHTL
                     AEIRAAYPDLPVDTETGDFVGVAGRVAFARNSGKLCFATMQEGDGTQLQTMISLAKVG
                     QESLDRWKSDVDLGDIVFVHGEVISSRRGELSVLADSWQIISKALRPLPVAHKEMSEE
                     SRVRQRYVDLIVRPEA"
     gene            551..1792
                     /locus_tag="VGP27_23810"
     CDS             551..1792
                     /locus_tag="VGP27_23810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV7854357.1"
                     /translation="MTAAIDAAGLAALADEPLDWRFKGLPASWWGRTPAQVCAVGPIL
                     GVGDMPGPLCVLRAGALSDNLQTMAGWCRDRGVELAPHGKTHMAPQLLARQFESGACA
                     VTVATISQARVYRAFGVRDFIVANELVDAAALRWVAAELAGDPAFALTCWVDSVAGVE
                     QMESHMAAAGRPVDVCVEIGMAGGRTGCRDASTADEVARAAAASPALRLVGVAGYEAA
                     LGHDVTPAAAARVTSYLSELRSAVERLADRFQTESVIVTAGGSTYFDAVAEVLTIGSV
                     AGRDVRTIVRSGCYLTHDHGLYARTSPLREGLRPALEVWAQVTSRPEHDLALLTMGRR
                     DVSFDQDMPVPISLSDSRVAKLNDQHAYLRLGPDDEPTVEVGDWLRFGISHPCTTFDK
                     WQMIPVLDDDDRVVELIRTFF"
     gene            complement(1789..2604)
                     /locus_tag="VGP27_23815"
     CDS             complement(1789..2604)
                     /locus_tag="VGP27_23815"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV7854358.1"
                     /translation="MLLAIDVRNTHTVVGLITGSGDHAKVAQHWRIRTETEVTSDELA
                     LTLDGLIGDDSERLTGAAGLSTVPSVLHEVREMLDQYWPSVPHVLIEPGVRTGIPLLV
                     DNPKEVGADRIVNCLAAFHKYGKAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSS
                     DAAAARSAALRRIELTRPRSVVGKNTVECMQAGAVFGFAGLVDGLVSRIRADIDGFGG
                     EDVAVVATGHTAPLLLPDLHTVADYDRHLTLDGLRLVFERNRDSQRGRLKQAR"
     gene            complement(2609..3016)
                     /gene="panD"
                     /locus_tag="VGP27_23820"
     CDS             complement(2609..3016)
                     /gene="panD"
                     /locus_tag="VGP27_23820"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEV7854359.1"
                     /translation="MLRTMLKSKIHRATVTQADLHYVGSVTIDADLMDAADLLEGEQV
                     TIVDIDNGARLVTYAITGERGSGVIGINGAAAHLVHPGDLVILISYGTMQDAEARQYQ
                     PRVVFVDAENKLTDFGHDPAHVPADAAELLSPR"
     gene            complement(3016..3966)
                     /gene="panC"
                     /locus_tag="VGP27_23825"
     CDS             complement(3016..3966)
                     /gene="panC"
                     /locus_tag="VGP27_23825"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV7854360.1"
                     /translation="MSGTVRKPPKFAAGNLNVYGSPRDVSDVTRALRQTGRRVMLVPT
                     MGALHDGHLTLIRAAKRVQGAVVVVSIFVNPLQFGANEDLDKYPNTLDDDLALLREEG
                     VQVAFTPTAAEMYPDGPRTSVQPGPLGAELEGAARPTHFAGVLTVVLKLLQTVRPDRA
                     FFGEKDYQQLVMIRQMVADLNVDAAIVGVPIVREPDGLAMSSRNRYLSDVEREQAGAL
                     SAALLAGMYAASGGTPAAVDAARAVLDEVPAIEVDYLEVRDVALGPAPTEGAARMLLA
                     ARLGATRLLDNIAIDIGSSAGAEGQWSAGSDDNHELPWRN"
     gene            complement(3963..4931)
                     /locus_tag="VGP27_23830"
     CDS             complement(3963..4931)
                     /locus_tag="VGP27_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEV7854361.1"
                     /translation="MEQSPSYPLGADPTHAWGAQDGPMRPARLSVGIISAGRVGTALG
                     VALERAGHVVVACSAISRASRQRAERRLPDTAVLPVHEVAIRAELLLLAVPDSELASV
                     VSGLAATHAVRPGTIVAHTSGANGIAVLSPLTEQDCIPLAIHPAMTFTGADEDIARLA
                     DTCFGITAADDVGYAVAQSLVLEIGGEPFRVREDARTLYHAALAHASNHVVTVVLDAV
                     EALRSALTGQELLGQELVGDDPGGIAERVIGPLARAALENALQRGQAALTGPVARGDA
                     GAVADHLRALSEANPELAQAYRANSLRTAQRAHAPEEVFAVLEEAG"
BASE COUNT          801 a         1725 c         1698 g          845 t
ORIGIN      
        1 gcctccggtc gcacgatcag gtcgacgtaa cgctgccgga cacgcgactc ctcactcatt
       61 tccttgtgcg cgacggggag tggcctaagc gccttggaaa ttatttgcca ggagtcggca
      121 agcacagaaa gttcaccgcg acgggaactg atcacttcgc cgtggacgaa gacgatgtcg
      181 cccaggtcga catccgactt ccatcggtcg agggactcct gtcccacctt ggccaggctg
      241 atcatcgtct gcagctgggt gccgtcgcct tcctgcatgg tggcgaagca cagcttgccg
      301 gagttgcgcg cgaaggcgac acggcccgcg accccgacga agtcgccggt ctccgtatcg
      361 acgggcaggt cgggataggc cgcccggatc tcggcaaggg tgtgcgtgcg cgcgacctcg
      421 accgggtagg gctcgcggcc ctcggcgagc agacgctcgc gcttggcctg acgaattcgg
      481 aactgctcgg ggatgtctgt ctcgtcgggc tgctcaggtg atgtcacgaa gtgccagctt
      541 aaatgaactc gtgaccgcag cgatcgatgc agccggcctc gccgccttgg cggacgaacc
      601 gctggactgg cgtttcaagg gtctgcccgc ttcctggtgg ggccgcaccc ccgcgcaggt
      661 ctgcgcggtt ggccccattc tcggcgtcgg tgatatgccc ggcccgctgt gtgtgctgcg
      721 tgccggggcc ctgtcggaca atctccagac catggccggg tggtgtcgcg accgcggagt
      781 cgagcttgcg ccccacggca agacccatat ggctccgcag ctgctggccc gccagttcga
      841 gtcgggagcg tgtgcggtca cggtcgcgac gatcagccag gcgcgtgtgt accgggcctt
      901 cggtgtgcgc gatttcatag tcgccaacga gttggtcgat gccgccgcac tgcgctgggt
      961 ggccgccgag ctggccggcg accccgcctt cgcgctgacg tgctgggtgg attcggtcgc
     1021 cggagtagag cagatggagt cgcatatggc ggccgctggt cgacccgtcg atgtctgcgt
     1081 ggagatcggc atggccggag gccgcaccgg ctgccgcgat gcttcgaccg ccgacgaggt
     1141 ggctcgggcc gcggcagcga gccccgccct gcggctggtc ggtgtggcgg ggtacgaggc
     1201 tgctctcggc catgacgtca ctccggcggc ggcggcgcgc gtcacctcat atctgtcgga
     1261 gctacggtcg gccgtcgaaa ggctggccga ccgtttccag accgagagtg tcatcgtgac
     1321 agccggcggc agtacgtact tcgacgcggt cgccgaggtg ctgacgatcg gctcggtcgc
     1381 ggggcgcgat gtgcgcacca tcgtccgaag cggctgctat ctcacccacg accacgggct
     1441 gtatgcgagg acatcaccac tacgcgaggg gctgcgaccg gcgctcgaag tgtgggcgca
     1501 ggtcacctca cggccagagc atgatctggc gctgctgacg atgggccgcc gcgacgtctc
     1561 gttcgaccag gacatgccgg tgcctatctc gctgtcggac agcagggtcg ccaagctcaa
     1621 cgaccagcac gcatacctgc ggctgggacc ggacgacgaa ccgaccgtcg aggtgggcga
     1681 ctggctgcgg ttcgggatct cgcacccgtg taccaccttc gacaagtggc agatgatccc
     1741 cgtgctcgac gacgacgacc gcgtcgtcga gctcatccgc acgttcttct aacgcgcctg
     1801 cttcagccgt ccgcgttggc tgtcgcggtt gcgttcgaac accagccgca acccgtcgag
     1861 cgtcagatgg cggtcgtagt cagccaccgt atgaaggtct ggcagcagca gcggggcggt
     1921 gtgaccggtc gcgacaacgg cgacatcctc accgccgaac ccgtcgatgt cggcacggat
     1981 ccgcgacacc agaccgtcga ccaggcccgc gaagccgaac accgcgccgg cctgcataca
     2041 ctcgacggtg ttcttgccga ccaccgaccg cggccgggtc agctcgatac ggcgcagcgc
     2101 cgccgaccgc gccgccgccg cgtcggacga gacctgtacg ccgggtgcaa ttgcaccgcc
     2161 gaggaactcc cctttggccg agaccacgtc gacgcatatt gacgagccga agtcgacgac
     2221 gatcgccgcc ttgccgtact tgtggaatgc cgcaagacag ttgacgatcc ggtcggcgcc
     2281 gacttccttc gggttgtcga ccagcagtgg gatgccggtt cgcacccccg gctcgatcaa
     2341 gacgtggggc accgacggcc agtactggtc gagcatctcc cgcacctcgt gcaacaccga
     2401 cgggaccgtc gacagaccgg ctgcgccggt gaggcgttcc gagtcgtcac cgatgaggcc
     2461 gtcgagcgtc aacgccagtt catcggacgt cacctcggtc tcggtccgaa tccgccagtg
     2521 ctgcgccacc ttggcgtggt cacccgatcc cgtgatcagg ccgaccacgg tgtgggtgtt
     2581 gcggacatct attgccaaca gcacgaggtc accgcggcga gagcagttcg gccgcgtcgg
     2641 cggggacgtg ggcggggtcg tggccgaaat cggtcaattt gttctcggcg tcgacgaaaa
     2701 cgacccgagg ctgatattgg cgggcctccg cgtcctgcat cgtgccgtag gagatcaaga
     2761 tgacgagatc accgggatgc acgagatgcg ctgccgcgcc gttgatcccg atcaccccgc
     2821 tgccgcgttc gccggtgatc gcataggtga ccagccgcgc gccgttgtca atgtcgacga
     2881 tggtcacctg ctcgccctcg agcaggtcgg cggcgtccat caggtcggcg tcgatcgtca
     2941 ccgatccgac gtagtgcagg tctgcctgag tcaccgtggc gcggtggatc ttcgacttca
     3001 gcatcgtccg taacatcaat tcctccatgg caattcgtgg ttgtcgtccg atccggccga
     3061 ccattgccct tcggcaccgg cggatgaccc gatgtcaatc gcgatgttgt ccagcagtcg
     3121 ggtggcgcca agtcgcgcgg ccagcagcat gcgcgccgcg ccctccgtcg gcgcaggccc
     3181 gagcgcgacg tcgcgcacct ccagatagtc gacctcgatg gcgggcacct cgtcgagtac
     3241 cgcgcgcgcg gcgtcgacgg cggcaggggt gccgcccgat gcggcgtaca tccctgccag
     3301 cagggccgcc gacagtgcgc ccgcctgttc gcgttccaca tcgctgaggt accggttgcg
     3361 cgacgacatc gccagcccgt cgggttcgcg aacgatcggc acaccgacga tcgcggcgtc
     3421 gacgttcagg tcggcgacca tctggcggat catcacaagc tgctggtagt ccttttcacc
     3481 gaagaatgcc ctgtccggcc ggacagtctg aagaagcttg aggacgacgg tcagcacccc
     3541 ggcgaaatgc gtcggccggg ccgcgccttc cagctccgcg cccagcgggc ccggctgtac
     3601 cgatgtgcgg ggtccgtcgg gatacatctc cgcggcagtc ggcgtgaagg cgacctggac
     3661 cccttcttcc cgcagcaatg ccaggtcgtc gtcgagggta ttggggtact tgtccagatc
     3721 ctcgtttgcg ccgaactgca gtgggttgac gaagatcgac accacgacga ccgcgccctg
     3781 cacccgcttg gcggcccgta tcagcgtcag gtgtccgtcg tgcagcgcgc ccatggtggg
     3841 caccaacatc acccgacgtc cggtctgccg caatgcgcgc gtcacatcgg acacgtcgcg
     3901 cggcgagcca tagacgttga ggttgcctgc ggcgaacttc ggcggcttgc gaacggtccc
     3961 gctcaccccg cctcctctag cacggcgaag acttcttcag gtgcgtgggc ccgctgcgcg
     4021 gtgcgcaacg agttcgctcg atatgcttgg gccagttcgg gattcgcttc gctcaaggcg
     4081 cgcaaatggt cggctaccgc acctgcatcg ccgcgggcca ccgggccggt cagcgccgcc
     4141 tgtccccgct gcagtgcgtt ctccagcgcc gcccgtgcca gtgggccgat cacccgctcg
     4201 gcgattccac cagggtcgtc accgaccagc tcctgaccga gcagctcctg acccgtcagc
     4261 gcggaccgca gagcttccac cgcgtcgagc accacagtga cgacgtgatt gctcgcatgc
     4321 gcgagcgccg cgtgatacag cgtgcgggcg tcttcgcgga cccggaacgg ctcaccaccg
     4381 atctcgagta ccagcgactg cgcgacggcg tagccgacgt cgtcggcggc ggtgatgccg
     4441 aagcaggtgt cggccagtcg ggcgatgtcc tcgtcggcgc cggtgaacgt catcgccgga
     4501 tgaatcgcca aagggatgca gtcctgttcg gtcagtggac tcagcaccgc gatgccgttg
     4561 gcgcccgacg tgtgcgccac gatggttccc ggtcgcacgg cgtgggtggc ggcgagaccc
     4621 gagaccacag acgccagctc ggagtccgga accgcgagca gcagcaactc cgcgcggatc
     4681 gcgacctcat gaacgggcag caccgcggtg tcgggcagcc ggcgctcggc ccgctgccgg
     4741 gaggcccggg atatcgcgct gcacgccacg actacgtgac cggcccgctc cagggccacg
     4801 ccgagcgcgg tgccgacccg gcctgccgag atgattccga ccgagagccg agccggacgc
     4861 atcggtccgt cttgggcacc ccacgcgtgc gtagggtcgg ctcccaacgg atacgaaggg
     4921 gactgctcca ttgcagaggg cctcgcgaga tctgtgtttc gttccggtcc tgcgtcgcgg
     4981 gtaccgaacg gtcgctaaga gactagctca ctcgtcgcgg cgtcgacgac gcccgccgcc
     5041 cgacgaaccg acttccagac gcgccagca
//
LOCUS       DASYLX010001009         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3027571,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001009 DASYLX010000000
VERSION     DASYLX010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027571"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1450
                     /locus_tag="VGP27_23835"
     CDS             101..1450
                     /locus_tag="VGP27_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7854362.1"
                     /translation="MAQAPRTRYAKCGDLDIAYQVFGDGPVDLVVLPGPMISIDSIDL
                     EPSLYRFHRRLGSFARVIRFDHRGMGLSSRMTDENAMGPRFWADDTLAVMNAAGSEQA
                     TIFGSGFTAMSGFVLAADHPERVRSLVMVNGVARTLWASDYQAGAEVSYADPFRTIVT
                     DPDAVERGFDALELIGPTVAADEAFRAWWDLAGNRAASPSMARAVSTVLLNSDVREYL
                     PRVTVPTLILHRDDSRFIPVGHGRYLAEHISGARYVELLGADALHWVGETGPMLNEIE
                     EFVTGAPSKTDAERVLTTIVFTDIVGSTQRAAGLGDMRWHDLLDNHDRIVRQQLLRFS
                     GREVNTAGDGFVATFTSPSVAIDCAKAIVEAVRPLGIEIRAGVHAGEVEVRGADIAGM
                     AVHIGARVGALAGPSEVLVSSTVREIVTGSRRLFAERGEHDLKGVPGRWRVYAVTGT"
     gene            complement(1456..2433)
                     /locus_tag="VGP27_23840"
     CDS             complement(1456..2433)
                     /locus_tag="VGP27_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854363.1"
                     /translation="MAIEKVSALVLDRLAVFEFGVICEVFGIDRSADGVPNFDFKVCG
                     PEPGKPIPTSVGATLTPDHGLDDLVGADLVAIPAIGGSDYRPEALQAVKAAADAGAII
                     LTVCSGAFVAGEAGLLDGRPCTTHWMHADDLARRFPTARVDRNVLFVDDGNLITSAGT
                     AAGIDACLHLVRRELGSEITNKIARRMVVPPQRDGGQRQYIDQPIPVRCSERFAPQLD
                     WILGNLDKPHTVATLARRANMSARTFARRFVDETGTTPMQWVTDQRVLYARRLLEESD
                     MDIDQIADRSGFGTATLLRHHFRRIIGVTPSDYRRSFACNMDGDRAQTA"
     gene            2505..2759
                     /locus_tag="VGP27_23845"
     CDS             2505..2759
                     /locus_tag="VGP27_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854364.1"
                     /translation="MTTALTFLILIAPFAVAAWLSWAAHRTDSLRIRFDQFRWSAPMV
                     GRLFEDDRDGFRIAHDVDAVRTRFEKQPSWPSSGATGERR"
     gene            complement(2733..>3477)
                     /locus_tag="VGP27_23850"
     CDS             complement(2733..>3477)
                     /locus_tag="VGP27_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854365.1"
                     /translation="GGHGDPLVLVHGLMGRGSTWTRQLPWLAEHGEVYTYDAPWHRGR
                     DVADPHPISTERFVDDLGDAVATLNRPATLIGHSMGALHSWCLAASRPDLVNALVVED
                     MAPDFRGRTTGPWEPWLHALPVEFESSKQVYDEFGPVAGQYFLEAFDRTATGWRLHGR
                     TKVWIDIAAQWGTRDYWVQWRNARVPALLIEAGNSVTPPGQMREMSETGRRATYLHVP
                     GAGHLVHDEQPQVYRDAVQSFLATLAGRA"
BASE COUNT          558 a         1169 c         1159 g          591 t
ORIGIN      
        1 cgcgccgtcg ggtcgtcccg gacgcggtcc aacgccgaac gcgcataggc cgcagcagaa
       61 ttgcccgcca catgccgatt cttccacgta gccttgacct gtggcgcagg caccgcgaac
      121 ccgctacgcc aagtgcggcg acctggacat cgcctaccag gtattcggag acggtcccgt
      181 tgatctggtg gtgctgcccg gaccgatgat ctcgatcgat tcgatcgacc tggagccatc
      241 gctgtaccgc ttccaccgcc gcctcggctc gtttgcccgg gtgattcggt tcgatcaccg
      301 cgggatgggt ctttcctccc gaatgacgga cgagaacgca atggggccgc ggttctgggc
      361 cgacgacacc cttgcggtga tgaacgccgc cggatccgag caggcgacca tcttcggctc
      421 cggcttcacc gcgatgagcg ggttcgtgct ggccgccgac catcccgagc gggtgcgcag
      481 cctggtgatg gtcaacgggg tggcgcgcac gctgtgggcg tccgactacc aagccggcgc
      541 cgaggtcagc tatgccgatc catttcgcac cattgtcacc gaccccgacg ccgtcgaacg
      601 tggcttcgac gccctggagt tgatcggacc gactgtggct gccgacgaag ccttccgggc
      661 ctggtgggac ctcgcaggca accgtgccgc gtcgccgagc atggcccgtg ccgtcagcac
      721 cgtgctgttg aattccgatg tgcgcgaata tctgccgcgc gtcacggtgc cgacgctgat
      781 cctgcaccgc gacgactccc gcttcatccc ggtagggcat ggccgctacc tcgccgagca
      841 catctctggc gcccggtatg tcgagctgct cggcgccgac gcgctgcact gggtgggcga
      901 aacgggaccg atgctgaacg agatcgagga gttcgtcacc ggagccccca gcaagactga
      961 tgccgaacgt gtgctcacca caatagtttt caccgacatc gtgggatcga cccagcgcgc
     1021 agccggactc ggggacatgc ggtggcacga cctgctcgac aaccacgacc gcatcgtccg
     1081 tcagcaactc ctgcggttct ccggccgcga agtcaacact gcgggcgacg ggttcgtcgc
     1141 gacattcacc agcccaagcg tggcgatcga ctgcgccaag gcgatcgtcg aggccgttcg
     1201 ccccctcggt atcgagatca gggccggggt acacgccggg gaggtcgaag tgcgtggtgc
     1261 cgacatcgcc ggaatggcgg tgcacatcgg tgcgcgggtc ggcgcactcg ccggtcccag
     1321 tgaagtgctt gtctcctcga ccgtacgcga gatcgtcaca ggatcacgac ggctgttcgc
     1381 cgagcgcggc gaacacgacc tcaaaggcgt gcccggccgg tggcgggtat acgccgtgac
     1441 cggaacctga ttcggctagg ccgtctgagc tcgatctcca tccatgttgc aggcgaagct
     1501 gcgacggtag tccgagggtg tcacgccgat gatccggcga aagtggtgac gcaacagcgt
     1561 tgcggtgccg aagcccgagc ggtcggcgat ctggtcgatg tccatgtcgc tctcttcgag
     1621 caaccgcctg gcgtagagca cacgttggtc ggtcacccac tgcatcggag tcgtgcccgt
     1681 ctcgtcgacg aagcgccgcg cgaaggtgcg cgccgacatg ttcgcgcgcc tggccaaggt
     1741 ggcgacggtg tgtggcttgt cgaggttgcc cagaatccaa tccagttgcg gtgcaaatcg
     1801 ttccgaacat cggaccggaa tcggctggtc gatgtactgg cgctggccgc cgtcgcgctg
     1861 cggcgggacg accatccggc gcgcgatctt gttggtgatc tcgctgccca gttcgcgacg
     1921 gacaaggtgc aggcacgcat cgatgcccgc cgccgtccct gcactggtga tcaggttgcc
     1981 gtcgtcgacg aagagcacgt tgcggtccac ccgggcggtc gggaaccggc gagccaggtc
     2041 gtcggcgtgc atccagtgcg tggtgcaggg ccgcccgtcc agcagccccg cctcgccggc
     2101 aacgaaggca cccgagcaca cggtcaggat gatcgcgccc gcgtcggccg cggccttgac
     2161 ggcctgcagc gcctcgggtc gatagtcgga tccgccgatc gccgggatcg cgacgaggtc
     2221 ggccccgacg aggtcgtcca acccgtggtc cggggtgagg gtggcgccga ccgacgtcgg
     2281 gatcggcttg cccggctccg gcccgcacac cttgaagtcg aagttcggga caccgtcggc
     2341 ggaccggtcg attccgaaga cctcgcagat cacgccgaac tcgaataccg caaggcggtc
     2401 gagcaccagc gccgacacct tttcaatggc catggcagta tcttaacgca cgctgtcact
     2461 gctgccactg ttggcgggat atttccccat ggagcattac tgccatgact acggcactaa
     2521 cgttcctcat tctgatcgca ccgttcgcgg tggccgcgtg gctgagctgg gccgcacacc
     2581 gcaccgactc acttcgcatc cggttcgacc agttccggtg gtccgctccg atggtcggaa
     2641 ggctgttcga ggacgaccgc gacgggttcc gcatcgcgca cgacgtcgac gccgtgcgca
     2701 cgcgcttcga gaagcagccc tcctggccgt cctcaggcgc gaccggcgag cgtcgctagg
     2761 aatgactgca ccgcatcgcg atagacctgc ggctgttcgt cgtggacgag atgaccggca
     2821 ccgggcacgt gtaaatacgt tgcccggcgg ccggtttcgc tcatttcacg catctgccca
     2881 ggcggcgtga cggagttgcc cgcctcgatg agaagtgcgg gcacccgcgc gttgcgccac
     2941 tgaacccagt agtcacgggt cccccactgc gcggcgatgt cgatccagac cttggttcgg
     3001 ccgtgcagcc gccacccggt tgcggtgcgg tcgaacgcct ccagaaagta ctgcccggcg
     3061 acgggtccga attcgtcgta aacctgtttg ctcgactcga attcgaccgg gagagcatgc
     3121 agccacggct cccacgggcc ggtggtgcgg ccgcggaagt ccggcgccat gtcctcgacg
     3181 acgagggcgt tcaccaggtc gggtctcgaa gccgccaagc accaggagtg cagggcgccc
     3241 atcgagtggc cgatcagggt ggcgggccgg ttcagcgtgg ccaccgcgtc gccgaggtcg
     3301 tcgacgaatc gttccgtgct gatcgggtgc ggatcggcga cgtcgcggcc gcggtgccac
     3361 ggcgcgtcgt aggtgtagac ctcaccgtgc tcggccagcc acggcagttg gcgcgtccat
     3421 gtgctgccgc gccccatcag gccgtgcacg agcaccagcg ggtcgccgtg accgcct
//
LOCUS       DASYLX010001010         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_614285,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001010 DASYLX010000000
VERSION     DASYLX010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614285"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VGP27_23855"
     CDS             <1..670
                     /locus_tag="VGP27_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEV7854366.1"
                     /translation="LTAKPVALTSVLPHQDSSAADQQWQRVTATGRYLADRQVLARLR
                     VVEGDPAFEVLVPFAVDGGPTVLVDRGYVKPEQGSRVPPIAPAPSDKVTIIARLRDSE
                     TAPTGKAPFRQDGAQQVYAIDTAQVSTLVGVPLAGSYLQLVDKQPGGLGVVPLPHLDA
                     GPFLSYGIQWIAFGIIAPIGLGYFIVSEIKARRREKAAAEARPASAPLTTEEKLADRY
                     GKRR"
     gene            complement(612..1589)
                     /locus_tag="VGP27_23860"
     CDS             complement(612..1589)
                     /locus_tag="VGP27_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEV7854367.1"
                     /translation="MCLGRAAGIAAGYAADLTFGDPRRGHPVALFGGAAARLERLTYA
                     DDRWAGAVHTGVLLGALTMAGVSAERAAARRGPAWTVVLTAATTFVALGGTSLARTGE
                     RMADLLACDDVDAARRLLPSLCGRDPAALDVDGLTRATLESVAENTSDAQVAPLLWAA
                     TGGVPALLLYRGANTLDAMIGHRSPRYRRFGWTAARFDDLMNIVAARVTGVLVALCAP
                     VAGGSPSDAVRAWRRDAGRHPSPNAGVAEASFAGALGVRLGGPTQYAHELEIRPALGD
                     GPAPRIVDLKRAVQLSRAVQFASAVLATVAVSVACRSGRRASLRWSKAR"
     gene            1637..2464
                     /locus_tag="VGP27_23865"
     CDS             1637..2464
                     /locus_tag="VGP27_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV7854368.1"
                     /translation="MRLGPPRRPTRVVDLINPAAVLAPAANVIMQLSLPGVGYGVLES
                     PVDSGNVYKHPFKRARTTGTYLAAATLGTDADRALIRDEVDKVHVLVRSKKSSPLSYN
                     AFDPRLQLWVAACLYRYFVDQHEFLYGPLDDEAADAIYLDARKLGTTLQVRDDMWPVD
                     RIAFDEFWKRMLDDLHIDPPVREHLHGVATMEFLPAPLRLLTGRFNLFATKGFLPPEF
                     RAHMKMSWTATQQRQFERLLTALRLADRVIPREVWIFGYRTYLWNMRARARRGKRVV"
     gene            2461..>2828
                     /locus_tag="VGP27_23870"
     CDS             2461..>2828
                     /locus_tag="VGP27_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854369.1"
                     /translation="MTALNHVAVTVRDIDASAPWYQALFDVDPVLDEKTDAGFRHVVW
                     AFDNGTLFGIHQHDRPLEGGRFTEFQAGLDHVGFGCADRAELDTWVEKLNKLGIEHGG
                     IVDAHYGSGLSFRDPDGIAL"
BASE COUNT          485 a          964 c          928 g          451 t
ORIGIN      
        1 gctgaccgcc aaacccgttg cgctgacaag cgttctgcca catcaggatt catcggcagc
       61 cgaccagcag tggcaacggg tgacggctac cgggcgctat cttgccgacc gccaggtgct
      121 ggcccggctg cgggtggtcg aaggcgatcc ggcgttcgag gtgctggtgc cgttcgcggt
      181 ggacggcggc ccgacggtgc tggtcgaccg cggatacgtg aagcctgaac agggttcgcg
      241 cgtcccgccc atcgcgccgg caccgagcga caaggtgacg atcatcgcgc ggctgcgcga
      301 ttccgagacg gctcccacgg gcaaggcgcc gttccgccag gacggcgctc agcaggtgta
      361 cgcgatcgac accgctcagg tatccacgct ggtgggtgtt ccgctggccg ggtcgtatct
      421 ccaactggtc gacaagcagc cgggtggcct cggcgtggtc ccgttgccgc acctggacgc
      481 cggccccttc ctttcctacg gcatccagtg gatcgcgttc ggcatcatcg cgcctatcgg
      541 gttgggttac ttcatcgttt ccgagatcaa ggctcgccga cgggagaagg ccgcggcgga
      601 ggccaggccc gctagcgcgc ctttgaccac cgaagagaag ctcgccgacc gctacggcaa
      661 gcgacgctga ccgccaccgt tgccaatacc gcgctcgcga attgaacggc acgcgacagt
      721 tgcaccgccc gtttcagatc gacgatccgc ggcgcaggcc cgtcgccaag ggcgggccgg
      781 atctccaact cgtgcgcgta ctgcgtcggc ccaccgagcc ggacgccgag cgcacccgcg
      841 aaagaagctt ccgctacgcc cgcgttgggg ctcggatgcc gaccggcgtc gcgccgccac
      901 gcccgtaccg catccgaggg tgacccgccg gccacgggcg cgcagagcgc caccagcaca
      961 cctgtgacac gggcggccac gatgttcata agatcgtcga atcgtgctgc ggtccaaccg
     1021 aatcgacgat agcgaggcga gcgatgaccg atcatcgcgt cgagggtgtt cgcgccgcga
     1081 tagagcagca gcgcaggcac gccaccggtc gcggcccaca gcagcggcgc cacctgggcg
     1141 tccgacgtgt tctcggcgac ggattcgagc gtagcgcgcg tcagcccgtc gacgtcaagc
     1201 gccgccgggt cgcgcccgca cagcgacggc agcaggcgcc tggcggcatc gacgtcatcg
     1261 cacgccagca ggtctgccat ccgttcaccc gtgcgcgcca gcgacgtgcc gccgagtgcc
     1321 acgaatgtcg tcgccgccgt gagaacgacc gtccatgcag gcccgcgtcg agccgcggcc
     1381 cgctctgcgc tgacgcccgc catcgtcagc gcaccgagca gcacgcccgt gtggactgcc
     1441 ccggcccacc ggtcatcggc gtaggtgagt cgctcgaggc gggcggcggc gccaccgaac
     1501 agtgccaccg gatgcccgcg ccgcgggtca ccgaacgtca ggtcggctgc gtagccggcc
     1561 gcgataccgg ccgcacggcc cagacacatc ggcgcaaaca cgctgcgagc gtctcacaaa
     1621 gtgatgtact ccaaagatga ggctgggccc accgcggcgg ccgacccggg tggtcgatct
     1681 gatcaatcct gcggcggtgc tggctcccgc agccaacgtc atcatgcagc tgtccctgcc
     1741 cggcgtcggc tatggcgtgc tggaaagccc ggttgacagc gggaatgtct acaagcaccc
     1801 cttcaagcgc gcgcgcacga ccggcaccta tttggccgcg gccacgctcg ggacggacgc
     1861 cgaccgggcg ctgatccgtg acgaagtcga caaggtgcat gtgctggtcc ggtcgaagaa
     1921 gtcaagcccg ttgtcttaca acgcattcga tccacgactg cagctctggg tggccgcctg
     1981 tctgtaccgg tacttcgtcg atcaacacga attcctgtac ggcccattgg acgacgaggc
     2041 cgccgatgcg atctacctgg acgccagaaa gctgggcacc acgctgcagg tccgcgacga
     2101 catgtggcct gtcgaccgca tcgcgttcga cgagttctgg aagcggatgc tcgacgacct
     2161 gcacatcgac ccgccggtgc gcgaacatct gcacggcgtg gccacgatgg aattcttacc
     2221 cgcgcctctg cggttactga caggtcgctt caatctcttt gccaccaagg gttttctgcc
     2281 gccggaattc cgggcgcaca tgaagatgtc atggactgcg actcaacagc gccagttcga
     2341 acggctgctg acggcgctgc gcctcgccga ccgggtcatt ccccgcgagg tatggatctt
     2401 tggctatcga acgtatctgt ggaacatgcg tgctcgcgca cgccgaggaa agagagtggt
     2461 atgacagcac ttaaccatgt cgcggtcacc gtgcgcgata tcgatgcgag cgcaccctgg
     2521 taccaagcgc tgttcgatgt cgatccggtt ctcgacgaaa agaccgatgc cggcttccgg
     2581 cacgtggtgt gggcgttcga caacggcacc ctgttcggta tccaccagca cgatcggccg
     2641 ttggagggtg ggcgattcac cgagtttcag gcgggcctcg accatgtcgg cttcggctgt
     2701 gcggaccgcg cggagttgga cacgtgggtc gagaagttga acaagctcgg tatcgagcac
     2761 ggcggcatcg tcgacgccca ttacggcagc gggctgagct tccgcgaccc cgacggcatc
     2821 gcgctcga
//
LOCUS       DASYLX010001011         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2370604,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001011 DASYLX010000000
VERSION     DASYLX010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370604"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGP27_23875"
     CDS             complement(<1..741)
                     /locus_tag="VGP27_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7854370.1"
                     /translation="MHRPKAILIAVVALLGISIVFGGAVAQKLGVGGFTDPAAESAQV
                     ADYLDQNFPTTPNLVLEVIARDGAINSPDVAVAADRVRQIVESESAARVVGLFNQQSA
                     ADLRSKDGHSGLVLVHVGGSLDEATKTAVRIIDKLPTDDPAVDVRAGGPLGVSVEIED
                     RVNHDLVISESIALPILLAVLVIVFGGLVAAFLPLAIGITSIVTTLLVLTILVSVTDV
                     SVHALTVATAFGLGLSIDFGLLMVSRFRE"
     gene            complement(762..1454)
                     /locus_tag="VGP27_23880"
     CDS             complement(762..1454)
                     /locus_tag="VGP27_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854371.1"
                     /translation="MRLFCFHHAGGGGAMFNAWNKALGPRVEVVGVETVNRERFATLR
                     QLVDEVNDQLGPALDGPHAFFGHSFGALLAYRLACLRVASGSPSPRALMVSSFAPPHL
                     PAPIPAVDHVDDHQLTALLSDLGGIPPELSEWPTLRDNAVAAARIDLRLCSTDEEDEP
                     VVLSCPIHAFGGSDDPLVTEADLQEWRSRTSGEFSVHVLRGGHFYLSDGPQLFATLLP
                     LLSRLPAVSEKC"
     gene            complement(1469..>1663)
                     /locus_tag="VGP27_23885"
     CDS             complement(1469..>1663)
                     /locus_tag="VGP27_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEV7854372.1"
                     /translation="SATALKLSNALLGRGINTNPIIYPAVPEDKARLRFFVTSCHTDE
                     QIRYTVKALAEELKLLTEAS"
BASE COUNT          328 a          530 c          522 g          283 t
ORIGIN      
        1 ttcccggaag cgcgacacca tcagcaatcc gaagtcgatg gacagcccga gaccgaacgc
       61 cgttgcgaca gtgagcgcgt gcaccgacac atcggtgacg ctcaccaaga tcgtcaacac
      121 caacagtgtc gtgacgatcg atgtgatgcc gatggccaga ggaaggaagg ctgcgacgag
      181 gccaccgaag acaatcacga gcacggcaag caagatgggc agcgcgatgc tctcgctgat
      241 cacgagatcg tgattgaccc ggtcctcgat ctccaccgac acgccgaggg gaccgccggc
      301 acgcacatcg accgccggat cgtcggtggg caacttgtcg atgatccgga ctgccgtctt
      361 tgtggcctcg tcaaggctgc cccccacgtg cacgaggacg aggcccgagt gcccgtcctt
      421 actacgcaga tcggctgcgg attgctggtt gaacaatccg accaccctcg ccgccgattc
      481 cgattccacg atctgccgca ctcgatctgc ggcgaccgcg acatccggac tattgatggc
      541 gccgtcgcgc gcgatgacct ccaacaccag gtttggcgtc gtggggaagt tttggtcgag
      601 gtagtcggcg acctgtgccg attccgcggc cgggtcggtg aaaccgccga caccgagctt
      661 ctgggccacc gcgccaccga atacgatgct gatgccgagg agagccacga cggcgatgag
      721 gatcgccttc ggtcggtgca ccgtgaaagt ggctaaccgg ctcaacattt ttcgctcacg
      781 gccggcagcc gggacaacag tggtagcagt gtggcgaaca attgcggccc gtcactgaga
      841 tagaagtggc cgccccgcag gacgtgcaca gagaactctc ccgatgtccg cgaacgccat
      901 tcctgaagat cggcttctgt caccagcggg tcgtcgctgc caccgaacgc gtggatgggg
      961 caggacagga ccaccggttc gtcctcctcg tccgtcgagc acaagcgcag gtcgatccgc
     1021 gccgctgcaa cggcgttgtc gcgcagggtg ggccactcgc tcagctcggg tgggatgccg
     1081 cccagatccg acagcaacgc cgtcagctga tgatcgtcaa cgtgatccac agcaggtatc
     1141 ggcgccggaa gatgtggtgg tgcaaaggaa gacaccatca gtgcgcgcgg cgaaggcgaa
     1201 cccgatgcca cccgcaggca cgccaacctg tatgccagca gtgcgccgaa gctgtggccg
     1261 aagaacgcgt gtggaccgtc aagtgccggt cccagttgat cgttcacctc gtccaccagc
     1321 tggcgcagcg tggcgaatcg ctcgcggttg acagtctcca ccccaacgac ttcgactcgg
     1381 gggccgagcg ccttgttcca tgcgttgaac atcgccccgc caccgccggc atgatggaag
     1441 cagaacaacc gcaccgtttc acccgccttc aggaggcttc cgtgaggagc ttgagctcct
     1501 cggcgagcgc tttcacggtg taccggatct gctcgtcggt atggcagctg gtgacaaaga
     1561 accgcagtcg cgctttgtct tccggcacgg ctggataaat gatcgggttg gtgttgatgc
     1621 cgcgtccgag aagcgcgttg gacagtttca gtgccgtcgc cga
//
LOCUS       DASYLX010001012         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1491882,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001012 DASYLX010000000
VERSION     DASYLX010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491882"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGP27_23890"
     CDS             <1..1068
                     /locus_tag="VGP27_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEV7854373.1"
                     /translation="NEDAADALTEHQAVVLDAVRLLNRVEVRGGAGSGKTFLAMEQAR
                     RLAQRGQRVALVCYSHGLASYLERISATWPRRQRPAYVGEFHALGVQWGAPQGPDESL
                     RTDETVQFWEHDLPRQMAELAMELEPGHRFDSIVVDEAQDFADSWWEPMLIALKDPDE
                     GGLYVFTDEGQRVFNRYGSPPVPLVPLILDHNLRNTRQIANAFQPLVDHPMRFLGGEG
                     PAVSFVACSRDEALIVGDDQIEALLEQGWRPEDLALLTTGGRHPEQTERQKEGHTAYW
                     DTFWDADQVFYGHVLGFKGLERRAVVLVVNEVSQFDRSRERLYVGLSRARDQLVVCGD
                     PDFVRGVGGPDLARRLNLPSS"
     gene            1196..2002
                     /locus_tag="VGP27_23895"
     CDS             1196..2002
                     /locus_tag="VGP27_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854374.1"
                     /translation="MAQTPIERLFIVEATNWKQAVISLLEPESSYTSWCCVADDAEEG
                     DAVAFVLNTDPTSVLTGLGYVGSDGDPTQAQVELSPIAPLGLVDLETLRMITGFRWNG
                     DPRHEWVLHGETAIRMTLALEDCRYRGDQYTRFGHNSMAAARVLLHSHGRCDGCDQRM
                     DLHFDDAGKYVHIHTVDAYHRGRPPEGAADWPGALCARCSARMRKDGYVSLVDYRLAQ
                     HPACPSCGAQQTRRALFGLLTSRDYPPWLVARGCCVTNDDWTCWQCGLAW"
     gene            complement(2044..3483)
                     /locus_tag="VGP27_23900"
     CDS             complement(2044..3483)
                     /locus_tag="VGP27_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV7854375.1"
                     /translation="MTSYHDRVEGVLLGTAAGDALGAPYEFLPPRGPELEVAMVGGGG
                     WERGEWTDDTAMAIAIAEVAATGADLRAAATQDTLVTRWLEWSHVTKDIGVQTSSVLR
                     AASRGGRITAADARAASAAFHQQTGRTSGNGALMRTAPVALAYLDDEDAMVEAARAIS
                     ELTHYDPDVGDACMLWCCAIRHAVLTGELDVRVGLRHIMSDRRAVWIKRLDDAEASAP
                     ASFPNNGWVVTAMQAAWSAIATTPVPVDDPDAGVFKADHLRLALDAAVRAGYDTDTVA
                     AIAGGLLGAAYGASAIPGAWRRVLHGWPDITAHDLVALATAIERKGKPDLFDYSYRGS
                     AIDTCARHPYDDRVLLGGIGVLRTLPAGVDAVVSLCRVSDDDMRDDMPHVEVRLTDRT
                     SEDENPHLDFVLLHTVRLIEQLRNEGRTVLVHCVAAYSRTPAVGALYGARLCSVDVDE
                     AVRDVTAVLPGASSNHAFRESLRRLQFAT"
     gene            3558..4211
                     /locus_tag="VGP27_23905"
     CDS             3558..4211
                     /locus_tag="VGP27_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7854376.1"
                     /translation="MSANKYRDPRGRRLTDYPRPSVAVDTAVLTLDPELGLVVLEVRR
                     PAGKGWALPGTFIHEGETLADAVDRSLRVKANVRGLHPRQLHVFDDPKRDDRGWVLSV
                     AHVQVVRREQLADRFAETTRLVSIDAPGRLPFDHADIIELAVDDVRMRYIDKPDPDGL
                     LGDEFTLRELRIAHETIAGRELQRDTFRRAMEPHLVATGATNVSGRGRPAELYRRAE"
     gene            complement(4331..>4956)
                     /locus_tag="VGP27_23910"
     CDS             complement(4331..>4956)
                     /locus_tag="VGP27_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854377.1"
                     /translation="VAYTTDDLRTLLREKLPCWRWAAFASVLVQRRAAVQGRLRDSAL
                     GFVGASGARLSSGPEVRYFVTERIEEIAALVGQVESFMLTPAFVDVFGDPGDEATADG
                     DGIAHAANRLMDYHDRFLDLSERCRSVSVPNQYSDLMRDVTAFADIPLQGYRTFIDDF
                     VERVGEMPELLRYANGTVEVDPVLLHMESDNQLLDRIFKRLKAIRGG"
BASE COUNT          872 a         1725 c         1510 g          849 t
ORIGIN      
        1 aacgaggacg ccgccgacgc gctcaccgaa caccaggccg tcgtcctcga cgctgttcgc
       61 ctcctcaacc gtgtcgaggt ccgtggcggt gccggcagcg gcaagacctt cctggcgatg
      121 gagcaggccc gccgactcgc ccagcgcggc cagcgcgtcg ccctcgtctg ctactcccac
      181 ggccttgcct cctacctgga acgcatcagc gccacctggc cgcgccgcca acggcccgcc
      241 tacgtcggtg agttccacgc cctcggagtg cagtggggag cgccgcaggg acccgacgaa
      301 tccttgcgca ccgacgagac cgttcagttc tgggagcatg acctgccgcg gcaaatggcc
      361 gagctggcaa tggaactcga acccggacac cgcttcgact ccatcgtcgt cgacgaggcg
      421 caggacttcg ccgactcgtg gtgggaaccc atgctgattg cgctgaagga tcccgacgaa
      481 ggcggcctgt acgtgttcac cgacgagggc cagcgcgtct tcaaccggta cggctcgccg
      541 ccggtgccgc tggttcccct gattctcgac cacaacctgc gcaacacccg ccagatcgcc
      601 aacgcattcc agccgctcgt cgatcacccg atgcggttcc tcggcggcga ggggccagcc
      661 gtgtcgtttg tcgcctgcag ccgcgacgag gcgctgatcg tcggcgacga ccaaatcgaa
      721 gcactcctcg aacagggttg gcgccctgag gatctcgcgc tgctgactac cggcggccgc
      781 catcccgagc agaccgaacg acagaaggaa gggcacacgg cgtactggga taccttctgg
      841 gacgccgacc aggtgttcta cggccacgtg ctcggcttca aaggactcga acgccgcgcc
      901 gtggtcctgg tcgtcaacga ggtcagtcaa ttcgaccgct cccgcgagcg cctctacgtc
      961 ggattgtcgc gagcacgcga ccagctagtg gtctgcggcg atcctgactt cgtccgtggc
     1021 gtcggtggtc ccgatctggc acgccggttg aacctgccat cgtcgtgatc gcaagaacta
     1081 tcggagtcga ccactagctt ccgactcaac ggctcacatc ggtcgggacg tgtaatcacc
     1141 cggcagctaa ctctcagccg tcccaaatgt catactgctg tggaagaatt gccttgtggc
     1201 gcaaactccc atcgaacggc tgttcatcgt cgaggcgacg aattggaagc aggcggtgat
     1261 ctccctactc gaacccgagt cctcatacac atcctggtgt tgcgttgcgg acgacgccga
     1321 agagggcgat gccgtcgctt tcgtgctcaa taccgacccg acctctgtgc tgaccgggct
     1381 tggctatgtc ggctccgacg gtgacccaac tcaagcccaa gttgagttgt caccgatcgc
     1441 cccgctcgga ctggtcgacc tcgagacact gaggatgatc accgggttcc gttggaatgg
     1501 agatccgcgt catgaatggg tgctacacgg cgagacggcg attcggatga cgctggcgct
     1561 ggaagactgc aggtatcgag gtgaccaata cactcggttc ggccacaact cgatggccgc
     1621 cgccagggtc ctgctgcact cgcacgggcg ctgtgacggc tgcgatcagc ggatggatct
     1681 tcacttcgac gacgccggta agtacgtcca cattcacacc gtcgacgcct atcaccgcgg
     1741 caggccgccc gagggcgcgg ccgactggcc aggcgccctg tgcgcacgat gctcagcacg
     1801 gatgcgaaag gacggatacg tcagcctcgt cgattatcgg ctcgcgcaac atcccgcctg
     1861 cccgtcgtgc ggcgctcaac agactcgccg ggcgctgttc gggttgttga ccagtcgtga
     1921 ttacccaccg tggctcgtcg cccgcggatg ctgcgtcacc aacgacgact ggacgtgctg
     1981 gcagtgcggg ctcgcctggt gagtgctgac acgactagcg cccgcggcga gtagctcgtc
     2041 gcgctacgtc gcgaactgga gacgccgcag cgactctctg aacgcatggt tggaagatgc
     2101 accgggcagc accgccgtca cgtcgcgcac ggcttcatcc acgtcgacgc tgcagagccg
     2161 cgcgccgtac agcgcgccca ctgccggcgt ccggctgtat gccgcgacgc agtgcaccag
     2221 cactgtgcgt ccctcgttcc gcaactgctc gatcagccgt actgtatgca gcaggacgaa
     2281 gtccaggtgc gggttctcgt cttcgctagt gcggtcggtc agccgtacct cgacatgcgg
     2341 catgtcgtcg cgcatatcgt catcgctcac ccggcagagc gacaccacag catcgacgcc
     2401 tgccggtagc gtccgcagta cgccgatgcc gccgagcagg acgcgatcgt cgtacgggtg
     2461 ccgtgcgcat gtgtcgatgg ccgagccacg gtacgagtag tcgaacagat ccggcttgcc
     2521 tttgcgctcg atcgccgtgg ccagtgccac caggtcgtgc gccgtaatgt ccggccagcc
     2581 gtgcagcacc cgccgccagg cgcccggaat cgccgacgcg ccgtacgccg cccccagcag
     2641 gccaccggcg atcgccgcca cggtgtcggt gtcatatccg gcgcggacgg ccgcgtccag
     2701 tgccagccgc aggtgatccg ccttgaacac accggcgtcg ggatcatcaa ccggcaccgg
     2761 cgttgtcgcg atcgccgacc acgccgcctg catcgccgtc accacccagc cgttgttggg
     2821 gaacgacgcg ggtgctgacg cctcagcgtc atccaagcgt ttgatccaca cggcgcgtcg
     2881 gtcagacatg atgtgccgca acccgactcg aacgtcgagt tcaccggtga gtaccgcatg
     2941 gcggatcgca caacaccaca gcatgcaggc gtctccgacg tccgggtcgt agtgggtcaa
     3001 ctcgctgatg gcacgggcgg cctccaccat ggcgtcttcg tcgtcgaggt aagcaagggc
     3061 caccggggca gtccgcatca acgcgccgtt gcccgaggta cggccagtct gctggtggaa
     3121 ggccgccgac gccgcccgcg cgtccgccgc cgtgatccgg ccaccgcgcg acgcagccct
     3181 gagcaccgag ctcgtctgga cgccgatgtc tttggtgacg tgcgaccact ccagccatcg
     3241 ggtcacgaga gtgtcctgcg tggccgccgc gcgcagatca gcgcctgtcg ccgccacctc
     3301 agcgatcgcg atcgccatcg ctgtgtcatc ggtccattcg ccgcgctccc acccgccgcc
     3361 gccgaccatg gccacctcaa gctccggccc gcgcggtggc aggaactcgt atggcgcgcc
     3421 cagcgcatct cctgccgcgg ttccgagcag cacaccctca acgcgatcgt ggtaagacgt
     3481 catgtctttg ctcctcaggt cggcaggttt gcgcatttct gcgctaattg cctatcacgg
     3541 tacgctatga ctccgacatg agcgcgaaca agtaccgaga cccccgcggg cggaggctaa
     3601 ccgactaccc acggccgtcg gtcgccgtgg acaccgcggt cctcacgctg gatcccgaac
     3661 tgggattggt ggtcctagag gtgcgtcgtc cggccggtaa ggggtgggcg ctgcccggca
     3721 ccttcatcca cgaaggggag acactcgccg acgccgtcga ccggtcactg cgcgtcaaag
     3781 ccaacgtgcg cggtctgcac ccgcggcaac ttcacgtctt cgacgacccg aagcgtgacg
     3841 accggggatg ggtgctgtcc gtggcccacg tacaagtggt ccggcgggag cagttggcgg
     3901 atcgcttcgc cgagacaacc cggctcgtgt caattgacgc gcccggccgg ctgccattcg
     3961 accacgccga catcatcgag cttgcagtcg acgatgttcg catgcgttac atcgacaagc
     4021 cggatcccga cggacttctc ggtgacgagt tcacgcttcg ggagctgcgc atcgcacatg
     4081 aaacgatcgc tggacgagag ctgcaacgcg acacctttcg acgtgcgatg gaaccacacc
     4141 ttgtcgccac cggcgcgacc aacgtcagcg gacgaggccg tcccgccgag ctgtaccgtc
     4201 gcgccgagtg accgaccgtc gcagccgccg gcacgggttc ttcggcacac cagggatgta
     4261 cagcgcagaa gttggctcta cggaccttga gtgaagggac tctacggcgc gaccgtcggt
     4321 caatctgccc tcagccgcca cgtatcgcct tcaaccgctt gaagatccga tcgagaagtt
     4381 gattgtcgct ttccatgtga agcagcacag ggtccacctc cactgtgcca ttcgcgtagc
     4441 gaagcagctc tggcatctct ccgactcgct ccacgaagtc gtcgatgaaa gtgcgatacc
     4501 cctgcagcgg aatgtcggcg aacgccgtca cgtcacgcat caggtccgag tactggttcg
     4561 gcaccgatac acttctgcat cgctccgaga ggtcgagaaa tcggtcgtga taatccatca
     4621 gacgattggc ggcgtgcgcg atcccgtcgc catcggccgt cgcctcgtct ccaggatcgc
     4681 cgaatacgtc cacgaatgct ggtgtcagca tgaagctttc gacctgacca accagcgcgg
     4741 cgatctcctc gatccgctcg gtgacgaagt accgcacttc tgggccagaa ctcagccttg
     4801 cgcccgacgc tccgacgaaa cccagcgcac tgtcccgtaa ccgcccctgc accgctgccc
     4861 gccgctgaac gagcaccgac gcgaacgcgg cccatcgcca acacggtagc ttctccctca
     4921 acagcgtcct cagatcgtct gttgtgtacg ccaccg
//
LOCUS       DASYLX010001013         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1381671,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001013 DASYLX010000000
VERSION     DASYLX010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381671"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VGP27_23915"
     CDS             complement(<1..217)
                     /locus_tag="VGP27_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEV7854378.1"
                     /translation="MNITVYTKPACVQCNATYKALEKQGVAYQKVDITLDSEARDYVM
                     ALGYLQAPVVVAGNEHWSGFRPDRIKSL"
     gene            801..2009
                     /locus_tag="VGP27_23920"
     CDS             801..2009
                     /locus_tag="VGP27_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEV7854379.1"
                     /translation="MSQTRFWKHDHNPVARCRWRDRKRYLWLLAPIVPSLAVGSWLLV
                     LLTGQAVFWWSCPIVAFVVIPVLDHLVGADAENPPEGALAQLKDDRFYRWVNFLYLPG
                     QYLSLVFACWIWANDWPDISLVDKLGLMVTVGIICGIAISSAHELGHQRALLERRLSK
                     LALAQSCYGHFFVEHNRGHHVRVATPEDPASSLLGESLYAFIRRSVTGGVRSAWQIER
                     ARLSRIGTSHWSLRNDVLNAWLLSLALFTVLAAWFGVVVLPWLIGQAVIGFCMLETVN
                     YLEHYGLRRQKLADGRYEPIRPSHSWNSNTIVANVCLFHLQRHSDHHAHPLRRYQTLR
                     HADEAPQLPSGYATMMLIAMIPPLWWRIMDPRVIDHYGGHLHRAALRQGYAPRARSGP
                     FGGKLADDVA"
     gene            complement(1968..2510)
                     /locus_tag="VGP27_23925"
     CDS             complement(1968..2510)
                     /locus_tag="VGP27_23925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEV7854380.1"
                     /translation="MADVKVLALVGSLRAASINRQLAELAAETAPDGVTVSLFEGLAD
                     LPFYNEDIDNDDVPNSVVALRAAAADADAALVVTPEYNGSIPGVLKNAIDWLSRPFGN
                     GALKGKPAAVVGGSFGRYGGVWAHDETRKTLGLAGPRVVEDLKLSVPFKSLDGKHPRE
                     NSEVAANLRDIVGKLAAEGA"
     gene            2600..>2794
                     /locus_tag="VGP27_23930"
     CDS             2600..>2794
                     /locus_tag="VGP27_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854381.1"
                     /translation="MSLGGLPVSVPQERGDAARNRTLLLEAARRLIAERGADAVSMDD
                     IAAAAGVGKGTVFRRFGSRAG"
BASE COUNT          474 a          919 c          872 g          529 t
ORIGIN      
        1 gcaggctctt gatgcgatcg ggacggaagc ccgaccagtg ctcattgccg gccaccacga
       61 ccggcgcctg caggtacccc agtgccatga cgtagtcgcg tgcctcggag tcgagggtga
      121 tgtccacctt ctggtaggcg acgccctgct tctccagcgc cttataggtg gcgttgcact
      181 gcacacatgc cggcttcgtg tagacggtga tgttcatctg cggtacggct cctctgcgga
      241 cggaactaaa acacggactg gcaactggtc aagtactgct cttcgcttag gttcagcgat
      301 ccgcctgccc tcaaaattcc ggccgcccgg gctgctgagc tgacgggtcg gaagaaccga
      361 ctctgccgga ttcagagctc cggccgaccc tcgaaagctt gggaattgtg ggggtctgtc
      421 ggtgtgccaa acactacacc tggtgtccga caatcagaag ggataccaca tcttcggaac
      481 aacattcatg aaattccctg gtcgtaagcc cagcggtggc ggatctctcg gcgtgtcgca
      541 catcacaatc cacgcgtggc gcccgccatc tagtggtgta ccaccgacca ccgacaggga
      601 tctgcggacg gatcgtcacg gtgacgcaac agcaaagccg ctgttggctc acgggagcgt
      661 ccggcttcga cgaccccaag actgacggtt cgcgcccgca acaatttgcc cgactcaccg
      721 agaaaccgcc gcgtgggagc cgttgatttg cgatagtgga ggtggtgtcg aagcagtaat
      781 cgggggggtc ggtgccgaga atgtcgcaga ccaggttctg gaagcatgat cacaaccctg
      841 tggcgcggtg ccggtggcgc gacaggaaac ggtacctctg gctactggcc ccgatcgtgc
      901 cgtcgctcgc cgtcggctcg tggttactgg tcctgctgac cgggcaggcc gtgttctggt
      961 ggtcgtgccc gattgtcgcg ttcgtcgtca ttccggtgct cgatcatctg gtcggggccg
     1021 acgccgagaa ccctccggag ggtgcactcg cgcagctgaa ggacgaccgc ttctatcggt
     1081 gggtcaactt tctctattta cccggccaat acctctccct tgtcttcgcc tgctggatct
     1141 gggccaacga ttggcccgac atctcgctgg tcgacaagct cggtctgatg gtcaccgtcg
     1201 ggatcatctg cgggatcgcg atcagtagcg cgcacgaact cggccaccag cgggccctcc
     1261 tggagcgcag gctgagcaag ctcgcactcg cgcaatcgtg ctacgggcat ttcttcgtcg
     1321 agcacaaccg cggacatcat gtcagggtcg ccacacccga ggatcccgcg agttcccttc
     1381 tcggagaatc cctttacgcg ttcatccggc gatcggtgac cggcggtgtg cgatctgcgt
     1441 ggcaaatcga gcgcgcccgg ctctcccgga tcggtacatc gcactggagc ctgcgcaacg
     1501 acgtgctcaa cgcctggctg ttgagcctgg cgttgttcac ggtgctggcc gcgtggttcg
     1561 gcgtcgtcgt gctgccgtgg ctcatcggcc aggccgtcat cggcttctgc atgctggaaa
     1621 ccgtcaacta tctcgagcac tacggcctgc ggcggcagaa gctggccgac ggccgctacg
     1681 aaccgatccg cccttcacat agttggaaca gcaacacgat cgttgccaac gtgtgcctgt
     1741 tccatctaca gcgtcactcc gaccatcacg cccatccgct gcgccgctac cagacgctgc
     1801 ggcacgcaga cgaggcgccg cagctgccgt ccggttacgc aaccatgatg ctgatcgcca
     1861 tgatcccgcc cctgtggtgg cgcatcatgg acccccgcgt catcgaccac tacggcggcc
     1921 acttgcaccg cgcggcgctg cgccagggct acgcgccgag ggcgaggtca ggccccttcg
     1981 gcggcaagct tgccgacgat gtcgcgtagg ttcgcggcga cttcggaatt ctcccgtggg
     2041 tgcttcccgt cgagcgattt gaacggcacc gacagcttga ggtcctccac cacacgcggg
     2101 cccgcgagtc ccaatgtctt gcgggtctcg tcgtgcgccc acacgccgcc gtaccggccg
     2161 aaggacccgc cgacaaccgc ggccggcttg cccttcagcg caccgttgcc gaacggtcgc
     2221 gacagccagt cgatggcgtt cttcagcaca ccgggaatgc tgccgttgta ttcaggggtg
     2281 acgacgaggg cggcgtcggc gtcggcggcg gcagcgcgca acgcgacgac ggaattcggc
     2341 acgtcgtcgt tgtcgatgtc ctcgttgtag aacggcagat cagccagccc ctcgaacaat
     2401 gacactgtga cgccgtccgg cgcggtctcc gcggccagtt ccgccagttg acggttgatc
     2461 gatgcggcac gcaggctacc cacaagcgcc aagaccttga cgtcagccat ttctcaatcc
     2521 cttcgatggg taatgtccat actcgcacga cacaaccgga ctgtggtccg ctttattccg
     2581 gctgcgttaa agtgcagaaa tgagtctcgg cggcctgccg gtgtccgtcc cgcaggagcg
     2641 gggcgatgcc gcacgcaacc gcacgttgct cctcgaggcc gcccgccggc tcatcgccga
     2701 acgtggtgcg gatgcggtgt cgatggacga tatcgccgcc gccgccggag tcggcaaggg
     2761 cacggtgttc cgccggttcg gcagccgggc cggg
//
LOCUS       DASYLX010001014         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_176248,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001014 DASYLX010000000
VERSION     DASYLX010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176248"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..627
                     /locus_tag="VGP27_23935"
     CDS             88..627
                     /locus_tag="VGP27_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007471758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tat pathway signal sequence"
                     /protein_id="HEV7854382.1"
                     /translation="MDLDRRSLLVAAFLTAAVAGCEKNAGRPAIHSISTTDPYPPDPN
                     ETFVLPHDKMAFKAWGGLPPRSGEMAMLYGDLNKQGPYLVMMRWHPGWFSAPHTYATD
                     RVCVVVSGTWWVNSGNDFLPNDAVPVGPGGYVKRTARTPHYDGVLAGRRNPAVIAIFG
                     LGPVDIQLVDPTEPSWHQV"
     gene            662..1540
                     /locus_tag="VGP27_23940"
     CDS             662..1540
                     /locus_tag="VGP27_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854383.1"
                     /translation="MSKSSVSRPLAGRVAVVTGAARGIGRAAAVALSGAGADVAGIDI
                     ASPVSDILDYEPATVDDLCETGRLVTSAGGRWSQHVADQRRIDEIRAAAEAVARERGG
                     VDIVFANAGIQAFKPLLEMSDPDWRDQIEVNLDGTANVLRVFAPLLVSRGGGRIIVTS
                     STQGQHGTKFGSSYSASKWGLLGLMKSAALELGVHNITVNAVIPGLVNTALTRHEDRY
                     AQAISDAGGTPSGDPVQDERTTIELMKKKTPLGVPWVEPEDVASLVVFLATDQAAMVS
                     GTAFAATAGDSAHVTA"
     gene            complement(1537..2313)
                     /locus_tag="VGP27_23945"
     CDS             complement(1537..2313)
                     /locus_tag="VGP27_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7854384.1"
                     /translation="MTSLTTARLGLRGTLNTHWYTTEIEAEVAVAKRPSRGSRRSAKD
                     RALDYVKNQILSGAFPGGELISEGEVADTLSMSRTPVREAFLRMEAEGLLRLYPQRGA
                     LVISVSPQEVRAVMEARLVLEQFAAGKVAGQSAEARGAVSDRMTSELARQRQTAAAGD
                     LREFSDADRAFHAILIEATGNSILHDVYNSLRDRQMRMVGESAVREPARMETILAEHM
                     QIAEALRDGDRNRSLDAIKAHLAGTQRTLGFAADAGDPAD"
     gene            2390..>3102
                     /locus_tag="VGP27_23950"
     CDS             2390..>3102
                     /locus_tag="VGP27_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEV7854385.1"
                     /translation="MSTSTELEPVDVGEDGLDPTVPAPMTNVAEYGFEGRFEDWAEDA
                     RYFEYSKAANPIGSGHTTQVPIKSFGRDLYLDGPTRIIPLDLSAELGISGGPATSPAL
                     VAHFVRIRGGDQIATSPNATSQLYYVISGRGFTAVNGRLARWEKGDFLTLPANSPATF
                     YADPGSDAAMYWVHDEPLLRHLGAEATEPRFRPTKFRRTEAVARLDEIASRPGANDKS
                     RVSVLLANAEEEQTLTITHV"
BASE COUNT          584 a          979 c          968 g          571 t
ORIGIN      
        1 tagtgccggc catcgggcgc cgacccgctc aaccgcgtcg tcatggctga ttggtaccaa
       61 gcagtccacg taccggaagg cggccccatg gatctggaca gacgctcctt actggttgcc
      121 gcattcctga cggcggcggt cgccggctgt gaaaagaacg ccggccgtcc ggctatccac
      181 tccatatcca cgactgaccc ctatcccccc gatcccaacg aaaccttcgt gctaccccac
      241 gacaaaatgg cattcaaagc ttggggcggc ttgccgccac gaagcggcga aatggcaatg
      301 ctctacggcg atctcaacaa gcaaggcccg tatctagtaa tgatgagatg gcatcccggt
      361 tggttcagcg caccacatac ctatgcgacc gatcgagttt gcgtcgtcgt gtccggcacc
      421 tggtgggtca acagtgggaa cgatttcttg cccaacgacg cagttcccgt tgggccaggc
      481 ggatatgtga aacgtactgc ccgcacaccg cactacgacg gggtgttggc cggtcgacgc
      541 aatcccgccg tgatcgcaat ctttggtttg ggccccgtcg acatccagtt ggttgacccg
      601 acagagcctt catggcacca agtttgagcg ctacctgtcc actgtcgctt cggagaaaat
      661 gatgagtaaa tcgagcgtgt cccggccatt ggccggtcga gtcgccgtgg tgacgggtgc
      721 ggcccgcgga atcggacggg cagccgctgt cgctcttagc ggtgccggcg cggacgtggc
      781 cgggattgac atcgcaagcc cggtgagcga catccttgac tacgagccgg ccaccgtcga
      841 cgacctgtgt gagaccggcc gactcgtgac gtccgcgggt ggccggtggt cacagcacgt
      901 cgccgatcag cgccggatcg atgagattcg cgctgcggcc gaggcggttg cacgggagcg
      961 gggaggcgtc gatatcgtct tcgcgaacgc cggcattcag gctttcaagc cgctactcga
     1021 gatgtctgac cccgactggc gggaccagat cgaggtgaat ctcgacggca cggcgaacgt
     1081 gttgcgggtt ttcgcgccgc tgctggtcag ccgcggcgga gggcggatca tcgtcacgtc
     1141 atcgacacag ggtcagcacg gaaccaagtt cggatcgtcg tactcggcct ccaaatgggg
     1201 actgctcggc ttgatgaagt ccgcggcact agagctgggc gtccacaaca tcacggtgaa
     1261 cgccgtcatc ccgggtctgg tcaacacggc gctgacccgg catgaggacc gctacgcaca
     1321 ggccatcagc gatgccggag gcacaccgag cggcgatccg gttcaggatg agcgcaccac
     1381 catcgaattg atgaaaaaga agacgccgct tggagttccg tgggttgaac ccgaggatgt
     1441 tgcttcgctg gtcgtcttct tggcaaccga tcaggcagcc atggtcagcg gtaccgcatt
     1501 cgccgccacc gccggtgaca gcgcacacgt caccgcctaa tcagccggat cccccgcatc
     1561 ggccgcgaaa cccagtgtcc tttgagtgcc cgcgaggtgt gccttgattg catcgaggga
     1621 tcgattacgg tcgccatctc gcagcgcttc ggcgatctgc atgtgttcag cgagaatcgt
     1681 ttccattcgc gcgggctctc gaacggcgga ttcaccgacc atgcgcatct ggcggtcgcg
     1741 aagcgagttg tagacgtcat gcagaatgct gttcccggtc gcctcgatga gaatggcgtg
     1801 gaaggcgcga tctgcatccg agaattcgcg caggtcccca gctgcggcag tctgacgctg
     1861 tcgagcgagc tcggaggtca tccgatcgga cacagcaccg cgcgcttcag cagattgccc
     1921 ggcgaccttc ccggcagcga actgttcgag aaccagcctg gcctccatga cggcgcgcac
     1981 ctcttgtggg gaaacggaga tcaccagggc gccgcgctgc ggatacagcc gcagcagtcc
     2041 ttcggcctcc atgcgcagga acgcctccct cactggggtg cgcgacatgc tcaatgtgtc
     2101 ggcgacctcg ccctcgctga tcagctcgcc ccccgggaag gcgccactga ggatctggtt
     2161 cttgacgtag tcaagcgctc gatccttcgc actgcgtcgg ctcccgcgac tgggcctttt
     2221 tgccacggcg acctctgctt caatctcagt cgtataccag tgcgtgttga gcgtaccgcg
     2281 caatcccaat ctcgcggtgg tgagcgacgt cactggccgg ctgacgtact actaactaag
     2341 atacaagttg tatctattaa caaagttgca ttctttgtag gaggagacaa tgtccaccag
     2401 cacagagcta gagccagtcg acgtcggtga ggacgggttg gatccaacgg ttccggcgcc
     2461 gatgaccaac gtggccgagt acgggttcga ggggcgcttt gaggattggg ccgaggacgc
     2521 gcgttacttc gagtactcca aggccgcaaa cccgatcggg tccgggcaca ctacgcaggt
     2581 tcctatcaag tccttcggcc gcgaccttta cctcgacggt ccgactcgaa tcatcccgtt
     2641 ggacctgtcc gccgaactgg gcataagtgg cgggcccgcg accagcccgg cgttggtggc
     2701 gcacttcgtg cggatccgcg ggggtgacca gatcgccacc agcccgaacg ccacctctca
     2761 gctgtattac gtgatcagtg gccgaggctt cacggccgtg aacggtcggc ttgcgcggtg
     2821 ggagaagggt gacttcctga cgctgcctgc caacagcccg gcgacgttct acgcggaccc
     2881 cggctccgac gccgcgatgt actgggtgca cgacgagccg ctgcttcgcc acctgggcgc
     2941 agaggccacc gaaccacgtt tccgcccaac gaaattccgc cgcactgagg ccgtggctcg
     3001 gctggatgag atcgcctcgc ggccgggcgc aaacgacaag agcagagtaa gcgtcctgtt
     3061 ggccaatgcc gaggaggagc agacactgac gatcacgcac gt
//
LOCUS       DASYLX010001015         6051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1603425,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001015 DASYLX010000000
VERSION     DASYLX010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603425"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /gene="leuA"
                     /locus_tag="VGP27_23955"
     CDS             <1..1416
                     /gene="leuA"
                     /locus_tag="VGP27_23955"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218227.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV7854386.1"
                     /translation="AIPDDVTIQVLTQCRPELIERTFTACEGAPRAIVHFYNSTSILQ
                     RRVVFRADREAVKAIATDGARMCVEQAKKYPGTLWRFEYSPESYTGTELEYAVDVCNS
                     VADIIAPTPDVPLIVNLPATVEMATPNVYADSIEWMHRHLNPRDSIVLSLHPHNDRGT
                     AVAAAELGYQAGADRIEGCLFGNGERTGNVCIVTLGMNLFSRGVDPQIDFSNIDEIRR
                     TVEYCNQLPVPERHPYGGDLVYTAFSGSHQDAINKGLDAMKVSADEQDADVDDLLWQV
                     PYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLALPRRLQIEFSQAIQKITD
                     GEGGEVSPKEMWDVFADEYLTPLRPLERMRQKVIASEVDGGTDTIEAVVKVNGDECEI
                     VGSGNGPLAAFVDALSTIGFDVSVLDYSEHALSAGEEAQAAAYVEASVGGKTVWGVGI
                     ATSITTASLRAVVSAVNRAAR"
     gene            complement(1435..1887)
                     /locus_tag="VGP27_23960"
     CDS             complement(1435..1887)
                     /locus_tag="VGP27_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854387.1"
                     /translation="MPDHRTVFNHVGLCVADRERSRRFYEGLLGFQFWWDLELPDEGT
                     AALLALDPPIGVRATYLIRDGFVLELIDYSKRDARTGAQRVMDEVGLTHVSLSVSDLA
                     GVLAQVESFGGTVIKESTTEHFAMIRDPDGQLIELLPDTWLANLPPRP"
     gene            1922..3184
                     /locus_tag="VGP27_23965"
     CDS             1922..3184
                     /locus_tag="VGP27_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854388.1"
                     /translation="MGLPDVSTDAIVDFDHHSDDYHRNELEINAELRRRCPVAWNENY
                     GGFWFVSDYASVAEVARDGETFAHKYEPDAADGVSYQGEMGVPRPEGQPPLGVGEVDG
                     PYHLALRRALTPFFSTGAVEKMRPFMEQSVHWFLDQRIADGQMDLVLDYASPVPAILT
                     MRLMGLPYDNWQRYANLFHSVMAAAGGTEYNEAIAEVPAMLDGLLEFIAARRADPRDD
                     LTSFLVQFDIDGKRLDDSQLLDILWNLIGGGVDTTTSLTALSLLHLGTHPDLRGQLID
                     RPELYRPAAEEFLRYFSVNKQLSRTVSRDTVLAGQRLRRNDRVLISWIGANHDEQEFE
                     CPGAVMFDRSPNRHVAFGLGPHRCIGAPLARVMFEVMVKAVLERMPDYEIDLVGVRRY
                     AGNPTMDGVGRLPVTFTAGRSLGTPRPE"
     gene            complement(3188..4177)
                     /locus_tag="VGP27_23970"
     CDS             complement(3188..4177)
                     /locus_tag="VGP27_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDDh family exonuclease"
                     /protein_id="HEV7854389.1"
                     /translation="MSHSWGRPAAEPGAGWAVVDVETSGFRPGQARIVSVAALAVGDD
                     GNVERSVSSLLDPGVDPGPTHVHGLTAEMLAGKPSFADVAPDLIEVLRGRTLVAHNVG
                     FDYSFLVAEAELAAVELPIDSVMCTVELARRLALDCDNMRLETLAEHWGVSQMRPHDA
                     LDDALVLAGILKPALLRARERKVWLPLRPVQRRQWPSGLVTHDELRPLKMIASRLPCP
                     YLNPGRFVRGRPLVQGMRVALSAEVARTHEELVERILHAGLAYTDAVDPETSLVICNE
                     ARPEQGKGYQANELGVPLVSDADFMDCLDGVIGGTGVEEFTDHTLDGGQYALF"
     gene            4252..5529
                     /locus_tag="VGP27_23975"
     CDS             4252..5529
                     /locus_tag="VGP27_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV7854390.1"
                     /translation="MVTARGRIALAAGAAARWSSRVTGRGAGAMIGGLVAMTLDKSIL
                     RQLAESRRTAIVTGTNGKSTTTRMTAAALGTLGPSEPRGRRHQTVATNAEGANMDAGL
                     VAALAGARAAQLAALEVDEMHVPHVADAVDPKVIVLLNLSRDQLDRVGEINHIERTLR
                     AGLARHPDTVVVANCDDVLMTSAAYDSPNVVWVAAGGSWANDSVSCPRSGEIIVRDGT
                     HWYSTGTDFKRPTPQWSYDDTTIYGPDGFSVPMRLALPGTVNRGNATQAVAAAVAMGA
                     DPAAAVTAASGVDEVAGRYRTVQLGAHTVRMLLAKNPAGWQEALSMVDTDAGSVVISV
                     NGQVPDGEDLSWLWDVNFEHFVKHSQTHPVIAAGERGTDLAVRLGYAGVEHTLVHDAV
                     EAITSCPPGHVEVVANYTAFLALNRTFGRGGRG"
     gene            5567..>6051
                     /locus_tag="VGP27_23980"
     CDS             5567..>6051
                     /locus_tag="VGP27_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV7854391.1"
                     /translation="MGTYGDGGNSVVLRKRLQLRGFDAEVVNITLDDPVPSTLDIYAL
                     GGAEDYAQRLATRHLIRYPGLQEAAANGAPVLAICAAIQVLGHWYETSAGERVDGVGL
                     LDLTTSPQPRRTIGEVVSQPLPAGLTERLTGFENHRGGTVLGPDAQPLARVVSGAGNR
                     EG"
BASE COUNT         1021 a         1990 c         2098 g          942 t
ORIGIN      
        1 gccatccccg acgacgtcac catccaggtg ctcacccagt gtcggcccga attgatcgag
       61 cggacgttca cggcgtgtga gggcgcaccc cgtgccatcg tgcacttcta caactcgacg
      121 tcgatcctgc agcgccgcgt cgtgttccgc gccgaccggg aggccgtgaa ggcgatcgcc
      181 accgacggcg cgcgcatgtg cgtcgagcag gccaagaagt atccgggcac gctgtggcgc
      241 ttcgaataca gcccggagtc ctacaccggc acggaactgg agtacgccgt cgacgtctgc
      301 aactccgtcg ccgacatcat tgcgcccact ccggacgtgc cgctgatcgt caacctgcct
      361 gccaccgtcg agatggccac cccgaacgtg tacgccgact cgatcgagtg gatgcaccgg
      421 catctgaacc cgcgggactc gatcgttttg agcctgcatc cgcacaacga tcgcggaaca
      481 gccgtcgcgg cagccgaatt gggctatcag gccggcgccg accgcatcga gggctgcctg
      541 ttcggcaatg gcgagcgcac cggcaatgtc tgcatcgtga cgctgggcat gaacctgttc
      601 tctcgcggcg ttgacccgca gatcgacttc tccaacatcg acgagatccg ccgcaccgtc
      661 gagtactgca atcaactgcc ggtacccgaa cgtcatccgt acggcggcga tctcgtctac
      721 accgcgttct ccggcagcca ccaggatgcg atcaacaagg ggctcgacgc gatgaaggtg
      781 tcggccgacg aacaggacgc cgatgttgac gacctcctgt ggcaagtccc gtatctgccg
      841 atcgatccgc gcgacgtcgg tcgcacgtac gaggcggtca tccgggtgaa ctcgcaatcc
      901 ggcaagggcg gcgtggccta catcatgaag gctgaccacg ggctggcgct gccgcggcga
      961 ctgcagatcg agttcagtca ggcgatccag aagatcaccg acggcgaggg cggcgaggtg
     1021 tccccgaagg agatgtggga cgtcttcgcc gacgagtacc tgacgccgct gcggccgctc
     1081 gagcggatgc ggcaaaaggt gatcgcctcc gaggtcgacg ggggcaccga caccatcgag
     1141 gccgtcgtca aggtgaacgg tgacgagtgc gagatcgtcg ggtcgggcaa cggtccgctt
     1201 gctgcgttcg tcgatgcgtt gagcaccatc ggtttcgacg tctcggtact ggactactcc
     1261 gaacacgcgc tctcggcggg tgaagaggcg caggcggccg catatgtgga ggcgtccgtc
     1321 ggcggcaaga ccgtatgggg cgtcgggatc gcgacgtcga tcaccacagc gtcgcttcgc
     1381 gcggtggtgt cggcggtcaa ccgcgccgca cgctaggccg cgctcaacag gccgctaggg
     1441 ccgcggcggc agattggcca gccaggtatc gggcagcagt tcgatgagct ggccgtcggg
     1501 atcgcggatc atcgcgaagt gctcggtcgt cgactccttg atgacggttc cgccgaagct
     1561 ctcgacctgc gccagcacgc cggccagatc cgataccgac agcgacacat gggtcaggcc
     1621 cacctcgtcc atcacccgct gggctccggt tcgggcgtcg cgcttcgagt agtcgatgag
     1681 ctccagcacg aagccgtcac ggatcagata cgtcgcacgg actccgatcg gcggatcgag
     1741 ggccagaagc gcggcggtgc cctcgtcggg cagttcgagg tcccaccaga actggaatcc
     1801 gagcagaccc tcgtagaacc ggcgcgaccg ttcgcggtcg gcgacgcaca ggccgacgtg
     1861 gttgaaaacc gttcggtgat cgggcacgac tccacctctc taaggatgca aagatagcgc
     1921 aatgggcctt ccggacgtca gcaccgacgc aatcgttgac ttcgaccatc attccgacga
     1981 ttaccaccgc aacgaactcg agatcaatgc cgaactacgc cgccgctgtc cggtcgcgtg
     2041 gaacgagaac tacggcgggt tctggttcgt cagcgactac gcctcggtcg ccgaggtggc
     2101 gcgcgacggc gagaccttcg cccacaagta cgaacccgac gctgccgacg gcgtgagtta
     2161 ccagggcgag atgggcgtgc cgcgcccgga aggccaacca ccgctgggcg tcggcgaggt
     2221 cgacggcccc tatcacctcg cactgcggcg cgcgctcacg ccgttcttct ccaccggcgc
     2281 cgtcgagaag atgcgcccgt tcatggagca gtccgtgcat tggttcctcg accaacggat
     2341 agccgacggc cagatggatc tggtgctcga ctacgccagc ccggtgcctg ccatcctgac
     2401 aatgcggttg atgggtctgc cctacgacaa ctggcagcgc tacgcgaacc tctttcactc
     2461 ggtgatggcc gcggccggtg gcaccgagta caacgaggcg atcgccgagg ttccggcgat
     2521 gctggacggt ctgctggagt tcatcgcggc ccgacgggcc gaccctcgcg acgacctgac
     2581 gagcttcctg gttcagttcg acatcgacgg caagcggttg gacgacagcc aactgctcga
     2641 catcctctgg aatctcatcg gcggcggggt cgacacgacg acgtcgttga ccgcgctgag
     2701 cctgctgcat ctcggcactc atccggatct gcgcggccag ctcatcgacc gcccagaact
     2761 gtatcggcct gcggcagagg agttcctgcg atacttctcc gtcaacaagc agctgagccg
     2821 gacggtcagc cgtgacacgg tgctcgccgg ccaacgactg cggcgcaacg accgggtcct
     2881 catcagctgg atcggggcga accacgacga gcaggagttc gagtgtcctg gcgccgtgat
     2941 gttcgaccgc tcacccaacc ggcacgtcgc gttcgggctc gggccgcatc gctgcatcgg
     3001 ggcgccgctg gcacgcgtca tgttcgaggt gatggtgaag gcggtcctcg agcgcatgcc
     3061 cgactacgaa atcgacctcg tcggcgtccg cagatatgcg ggcaacccaa cgatggacgg
     3121 cgtgggcagg ctgcccgtga cgtttacggc cggacggtcg ctggggacgc cgcggcccga
     3181 gtgagcgtca gaagagggcg tactggccgc cgtcgagcgt gtggtcggtg aactcctcga
     3241 cgccggtgcc gccgatcacg ccgtcgaggc agtccatgaa gtcggcgtcg gagaccagcg
     3301 gcacgcccag ttcattggcc tggtagccct tgccctgctc gggtcgggcc tcgttgcaga
     3361 tcaccaacga ggtctccggg tcgacggcgt cggtgtaggc cagcccggcg tgcaggatcc
     3421 gctcgacgag ttcctcatgg gtgcgggcca cctcggccga cagcgcgacg cgcatgccct
     3481 gcaccagggg ccgcccgcga acgaagcggc ccgggttcag atacgggcac ggcagccgcg
     3541 acgcgatcat cttgagtggg cgtagctcgt cgtgggtgac gagcccgctg ggccactgtc
     3601 gccgctgcac gggtcgcagc gggagccaga ccttgcgttc acgcgcgcgc aggagtgccg
     3661 gtttgaggat ccccgccagc accagggcgt cgtccagtgc gtcgtgtggc ctcatctggc
     3721 tgactcccca gtgctcggcc aacgtctcga gccgcatgtt gtcgcagtcg agggcgagtc
     3781 gccgggccaa ttcgaccgtg cacatgacgg agtcgatcgg cagttccacc gcggccagct
     3841 ccgcctcggc gaccagaaac gagtagtcga accccacgtt gtgcgcgacg agggtgcgcc
     3901 cgcgcagtac ctcgatcagg tcgggggcga cgtcggcgaa ggacggctta ccggcgagca
     3961 tctccgcggt cagaccgtgt acatgggtgg gcccgggatc gacaccggga tccagcaggc
     4021 tggagacgct gcgctccacg ttgccgtcgt cgccgaccgc cagcgcggcg acggagacga
     4081 tgcgggcctg ccccggtcgg aagcctgacg tttccacgtc gacgaccgcc cagcccgcgc
     4141 cgggttcggc agccggccgg ccccaactgt ggctcacatc gtcaggatgg cacggccatc
     4201 cgacaaggct cggccggtgg cgccgcgtgt cgggcaccta aaatgccggt catggtcacc
     4261 gctcgtggac gcatcgcgct ggcggccgga gccgccgccc gctggtcgtc gcgggtgacg
     4321 ggccgcggcg caggcgcgat gatcggcggt ctcgtcgcga tgacgctgga caaatccatc
     4381 ctccgtcagc tggccgaaag ccggcgaacg gccatcgtga ccggcaccaa cggcaagtcg
     4441 acgaccacca ggatgaccgc tgcggcactg ggcacgctgg ggccttcgga gccgcgaggg
     4501 cggcgacatc agacggtcgc gacgaacgcc gagggcgcca acatggacgc cggactggtc
     4561 gccgcgctgg caggcgcgcg cgcggcgcaa ctggccgcgc tcgaggtcga cgagatgcat
     4621 gtgccgcacg tggccgatgc ggtcgacccg aaggtgatcg tgctgctgaa cctgtcgcgg
     4681 gaccagctgg accgggtcgg tgagatcaat cacatcgagc gcacactgcg ggccgggttg
     4741 gcccgccacc ccgacaccgt cgtcgtcgcc aattgcgacg acgtcctgat gacgtcggcg
     4801 gcgtacgaca gcccgaacgt ggtctgggtc gccgcaggcg gcagttgggc gaacgattcg
     4861 gtgagctgtc cgcgcagcgg cgagatcatc gtccgcgacg gcacgcactg gtactcgacg
     4921 ggaacggatt tcaagaggcc gacgccgcag tggtcatacg acgacacgac catctacggg
     4981 cccgacggat tctcggtgcc gatgcggctg gcattgccgg gaacggtcaa ccgcggcaac
     5041 gcgacgcaag cggtggccgc ggcggtcgcg atgggagccg accccgcggc ggcggtgacg
     5101 gcggcgtcgg gtgtcgacga ggtcgcgggc cgctacagga ctgtccaact cggcgcgcac
     5161 accgtgcgga tgctgctggc gaagaacccg gcgggctggc aggaagcgct gtcgatggtc
     5221 gacaccgacg cgggctcggt ggtgatctcg gtcaacggcc aggtgcccga cggggaggac
     5281 ctgtcgtggc tgtgggacgt caacttcgag cacttcgtga aacactcgca gacccatccg
     5341 gtgatcgccg ccggtgagcg cggcaccgat ctggccgtgc gactcggcta cgcgggcgtc
     5401 gagcacacgt tggtgcacga cgccgtcgag gccatcacgt cgtgtccgcc cggtcacgtc
     5461 gaggtggtcg cgaactacac ggcgtttttg gctttgaacc gcaccttcgg caggggcggc
     5521 cgtggctgac tcgacggtgc ggatcgggct ggtgctgccc gatgtgatgg ggacatacgg
     5581 cgacggcggc aactcggtag tgctgcgaaa gcggttgcag ctgcggggtt tcgatgccga
     5641 agtcgtcaac atcacgctgg acgacccggt gccctcgacc ctggacatct acgcgctcgg
     5701 cggagcagag gattatgcgc agcggctcgc gacccgccac ctgatccggt atcccggtct
     5761 acaggaggcg gcggcgaacg gcgctccggt gctggcgatc tgcgcggcga tccaggtgct
     5821 cgggcactgg tatgagacat cggcgggtga gcgggtcgac ggcgtggggc tgctggacct
     5881 gacgacctca ccgcagccga ggcgcaccat cggcgaggtg gtgagtcagc cgttgcctgc
     5941 cggactcacc gagcggctga ccggcttcga gaaccaccgc ggcggcacgg tgttggggcc
     6001 ggatgcgcag ccgttggcac gggtggtgtc gggcgcgggc aatcgcgagg g
//
LOCUS       DASYLX010001016         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1053288,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001016 DASYLX010000000
VERSION     DASYLX010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053288"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VGP27_23985"
     CDS             <1..945
                     /locus_tag="VGP27_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEV7854392.1"
                     /translation="SATLVIKPGWGFGFNYQPGQYVGIGLLVDGRWRWRSYSLTSSPI
                     KAGPGRQVTSKTSRTITITVKAMPEGFLSTHLVGGVEPGTIVRLAAPQGNFVLPDPAP
                     ASVLFITGGSGITPVMSMLRTLVRREQITDVVHLHSAPTESDLMFAEELAELQQSHPG
                     YRLLLRMTREHGRLDLSRIADEVPDWRDRQTWACGPEGMLSATESAWSTAGLKEQLHL
                     ERFAAARTAVHGQGGTVEFARSGKTVTVDAATPLMEAGEGAGVQMPFGCRMGICQSCV
                     VGLLDGHVRDLRSGVEHEPGSRVQTCISAASGDCVLDA"
     gene            1041..>1758
                     /locus_tag="VGP27_23990"
     CDS             1041..>1758
                     /locus_tag="VGP27_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV7854393.1"
                     /translation="MAITDVPEFAHLTEADIEHLAVELDAIREDIEDSRGARDARYIH
                     RTIAAQRALEVAGRVMLAAGSKRSAWWAGTVTLGLSKIIENMEIGHNVMHGQWDWMND
                     PEIHSSTWEWDMSGASKHWRFTHNFMHHKYTNILGMDDDVGYGVIRVTRDTKWKPFNL
                     YGNLLFNTLLAIGFEWGVGLQHLELGKISKGRHDRKATLERVGEFGVKAGHQVAKDYI
                     AYPALTSLSPGATFFSTLKAN"
BASE COUNT          315 a          554 c          595 g          294 t
ORIGIN      
        1 tccgcgacgc tggtgatcaa gccgggctgg ggcttcggct tcaactacca gcccggccag
       61 tacgtcggaa tcgggttgtt ggtggacggc cgctggcggt ggaggtcgta ctccctgaca
      121 tcgagcccga tcaaggccgg acccggtcgg caggtgacat ccaagaccag tcgcaccatc
      181 acgatcaccg tcaaggccat gcccgagggg ttcctgtcga cgcatctcgt cggcggggtg
      241 gaacccggca cgatcgtgcg gctggccgcg ccgcagggca atttcgtgct gcccgatccg
      301 gcgcccgcgt cggtgttgtt catcaccggc gggtcgggaa tcacgccggt gatgtccatg
      361 ctgcgcacgt tggttcggcg cgaacagatc accgacgtcg ttcatttgca ttccgcgcca
      421 acggaatccg acctgatgtt cgccgaggag ctggccgagt tgcagcagag ccaccccggc
      481 taccggctgc tgctgcgcat gacgcgcgag cacggccgcc tcgacctgtc gcggatcgcc
      541 gacgaggtgc ccgactggcg cgaccggcag acctgggcct gcgggcccga gggcatgctg
      601 agtgccaccg aaagcgcatg gtcgacggcc ggcctcaagg agcagttgca tctggagcgg
      661 ttcgcggccg cacgcacggc cgtgcacgga cagggcggca cggtggaatt cgcccgaagc
      721 gggaagacag tgacggtgga tgccgccacg ccactgatgg aggccggtga aggcgcgggc
      781 gtgcagatgc cgttcggctg ccggatgggc atctgtcagt cctgtgtggt cggactgctc
      841 gacgggcacg ttcgcgacct gaggtcgggc gtcgaacacg agccgggcag ccgggtgcag
      901 acctgcattt ccgcagcttc cggcgattgt gtgctggacg cttagcggcc gcgaggagca
      961 tggacaccag aactttactg gctggtaacc tacgacgccg taggttacgg tagcgtaggt
     1021 atcgaggaag gaggcagaca atggctatta ccgacgttcc ggaattcgca catctgaccg
     1081 aagccgacat cgagcatctc gctgttgagc tggatgccat ccgcgaggat atcgaggact
     1141 cccgtggtgc gcgcgatgcg cgctatatcc accgcaccat cgccgcccag cgtgcgctcg
     1201 aggtggcggg ccgggtcatg ctggccgccg gttcgaaacg ctccgcctgg tgggcaggga
     1261 cggtcacgct gggcctctcc aagatcatcg agaacatgga gatcggccac aacgtcatgc
     1321 acggccaatg ggactggatg aacgatcccg agattcactc ctcgacatgg gagtgggaca
     1381 tgagcggggc gtccaagcac tggcgcttca cccacaactt catgcaccac aagtacacca
     1441 acatcctcgg catggacgac gacgtgggct acggcgtcat ccgggtcacc cgcgacacca
     1501 agtggaagcc gttcaacctc tacggcaatc tgctgttcaa caccctcctc gcgatcgggt
     1561 tcgagtgggg cgtgggcctg cagcacctcg agctcgggaa gatctccaag ggccgccacg
     1621 atcgcaaggc gacgctggag cgggttggtg agttcggcgt gaaggccggc catcaggtgg
     1681 ccaaggacta catcgcctat ccggcgctga cgtcgctgtc gcccggcgcg accttcttct
     1741 cgacgctcaa ggccaacg
//
LOCUS       DASYLX010001017         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1492158,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001017 DASYLX010000000
VERSION     DASYLX010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492158"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGP27_23995"
     CDS             <1..444
                     /locus_tag="VGP27_23995"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEV7854394.1"
                     /translation="VGSDDAGYEYKEALKGDMRGDSRVAEVTDVGVGADEDTAYPHVA
                     VAAARMVAEGKADRALLVCGTGLGVAISANKVPGIRAVTAHDSFSVERSVLSNDAQVL
                     CLGQRVIGLELARRLAREWLGYEFDPESKSADKVEAIRGYEPVVP"
     gene            525..1358
                     /locus_tag="VGP27_24000"
     CDS             525..1358
                     /locus_tag="VGP27_24000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEV7854395.1"
                     /translation="MSTASVLIAADPPAVAGTAPRYTLLLSTDPALIEAAQRLRHDVF
                     TSEPGFALDGATDGKDADRFDEFCDHLLVREDDTGELVGCYRMLPPPGAIAAGGLYTA
                     TEFEVSGLDPLRPSLVEMGRAVVRDDHRSGAVVLLMWAGILAYLDRCGYDYVTGCVSV
                     PVDVSDGAPGSQIRGVRDFVLRRHAAAGEYTVYPYRPVTIGGKGLDDIDPPARLGVPP
                     LMRGYLRLGAQVCGEPAHDLDFDVGDFPALLDKRRADTRYLKRLRSVAAASEMADGIA
                     S"
     gene            1355..>1666
                     /locus_tag="VGP27_24005"
     CDS             1355..>1666
                     /locus_tag="VGP27_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7854396.1"
                     /translation="MTAAQDASPWVPRATCDASCVAAGGAASGRPVVVAVRVAVRVTL
                     AVIALSAVPLLAIPLPGRSHIQRGYCRMMLRALGVRITVSGGPIRNLRGVLVVSGHVS
                     WV"
BASE COUNT          239 a          549 c          593 g          285 t
ORIGIN      
        1 gtcggatcgg acgacgccgg ctatgagtac aaggaggcgt tgaagggtga catgcgcggc
       61 gactcgcgcg tcgccgaggt caccgatgtc ggcgtcggcg ccgacgagga caccgcgtac
      121 ccgcatgtgg ccgttgcggc ggccaggatg gtcgccgaag gaaaggcgga ccgcgcgctg
      181 ctggtgtgcg gcacgggcct cggtgtggcc atcagcgcga acaaggttcc cggcatccgg
      241 gccgtgaccg cccacgacag cttctccgtc gaacggtcgg tgctgtccaa cgatgcccaa
      301 gtgctgtgcc tcgggcaacg ggtgatcggg ctggagctgg cccgccggtt ggcacgtgag
      361 tggctgggtt atgaattcga cccggaaagt aagtcggcgg acaaggtcga ggccatccgc
      421 gggtacgaac cggtcgttcc gtagcgcaaa tcgtcacctg gcgtgcacag acacgtcatg
      481 tcccggatgc cgtttcgctg aacgactccg cgaacgtgca ggctatgagc actgcatctg
      541 tactcatcgc cgctgatccg cctgctgtcg cgggcaccgc tccgcgctac accctgctgc
      601 tgtccaccga ccctgcgctc atcgaagccg cgcagcgcct tcgtcatgac gtattcacct
      661 ccgaaccggg gttcgcgctc gacggcgcca ccgacggaaa agacgccgac cggttcgacg
      721 agttctgcga tcaccttctg gtccgcgagg acgacaccgg cgagctggtc ggttgctatc
      781 gcatgcttcc gccaccgggc gcgatagccg ccggaggcct ctacaccgca accgaattcg
      841 aggtctccgg ccttgacccg ctgcggccgt ccctggtcga gatgggccgc gccgtggtcc
      901 gcgacgacca ccgcagcggc gccgtcgtgt tgctgatgtg ggcgggcatc ctggcctacc
      961 tggaccggtg cggttacgac tacgtcacgg gttgtgtgtc ggtgcccgtt gatgtttcgg
     1021 acggggcacc gggcagccag atccgcggtg tgcgcgactt cgtgctgcgc aggcatgccg
     1081 ccgccggcga gtacacggta tacccgtacc ggccggtgac gatcggcggc aagggcctcg
     1141 acgacatcga tccgcctgcg cggctgggtg ttccgccgct gatgcgcggg tatctgcggc
     1201 tcggcgctca ggtgtgcggt gagcccgccc acgatctgga cttcgatgtc ggcgacttcc
     1261 cggcgctgct cgacaaacgc agggccgaca cgagatatct caaacggctg cggtcggtcg
     1321 ccgcggcgag cgagatggcc gacgggatcg cgtcatgacc gccgcacagg acgccagccc
     1381 ctgggtccct cgcgcgacgt gcgacgccag ctgcgtggct gcgggaggtg cggccagcgg
     1441 caggccggtc gttgtggcgg ttcgggtcgc cgtccgggtg acgctcgcgg tcattgcgct
     1501 ctcggcggtt ccgctgctgg cgataccgct gcccggccgc tcgcacatcc agcgcgggta
     1561 ttgccggatg atgctgcggg cgctcggcgt acggatcacg gtgtcgggcg gcccgattcg
     1621 aaacctgcgc ggcgtgctgg tcgtcagcgg gcacgtgtcg tgggtc
//
LOCUS       DASYLX010001018         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2152094,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001018 DASYLX010000000
VERSION     DASYLX010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152094"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VGP27_24010"
     CDS             complement(<1..1797)
                     /locus_tag="VGP27_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV7854397.1"
                     /translation="METFTPSLDWGDELVASALWVAKAWVFAAIGMVVVCALLARHTT
                     WGRQFWRVTGDYFTGRQSISVWGLLGVLLVLVMIDVRLVVLFTYQSNDQFSAMQAAFE
                     GEGVAKGAAIHGFWFAILILAALVVAKVVRTLLDTYLMRRFIIRWRVWLTHRLTGDWL
                     DGDAFYRGRFIDQPIDNADQRIQQDIDIFTTGTGPETNTPTVGTAQTLLFGTIFAIVS
                     VVEFTPILWGLSGPLTVLGLTLPKALFWIALLYVFLTTVVAFWIGRPLIRLSFRNELT
                     NAAFRYALIRLREAGEAVGLYRGEDAERGELMTRFAAVIANYRAFVRRGIAFLGWNRS
                     MNEIVDPLPTIIQAPRLFAGQIQLGDVTQSSSAFNQLQGSLSFFRAVYDAFAGYRAAI
                     IRLHGLVTANETARALPSLTALPSNDGSLELNNVEVRTPAGIRLIDPLDVRLDPGESL
                     VISGTSGSGKTTLLRSLAQLWPFTSGTLRRPQDDTMFLSQLPYMPLGDLRSTLSYPAG
                     AGSYPDDAVNSALDAVSLAHLGTRLNEVADWAKVLSPGEQQRIAFARVLLAKPKAVFL
                     DEATSALDEGQEFALYRALRTQLPDCIVVSVSH"
     gene            complement(1821..3755)
                     /locus_tag="VGP27_24015"
     CDS             complement(1821..3755)
                     /locus_tag="VGP27_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV7854398.1"
                     /translation="MESEMWAPTLDWGSELWVSLIWLAKAWAIAAVATLVICVLIGRF
                     TVWGRQFWRITGAYFSGAESVKVWLWLGLILFVVMLGVRVDVLLSYYSNDMSNAFQAV
                     AGGLSTNNEALKQSGKHGFYFSMAVFCVLAALFIFQLLVDMFMVQRFCLAWRAWLTDR
                     LTGDWLDGKAYYRSRFIDDKIDNPDQRIQSDIDIFTANNGPLPNVPYYSSQNTLLFGA
                     ISAVASIVSFTAILWGLSGPLTLPLIHFSLPRAMFWIGIVFVLFASVIAFWVGRPIIW
                     LSFSNEKFNAAFRYALVRLRDSSEAVAFYRGEVAERTGLRKLFRPVVDNYKRYVLRSL
                     GFNGWNWSMGQLIVPLPYLLQFPRFYSGDILLGAMSQSASAFGSIQTGLSWFRNAYDL
                     FAGYRAAIIRLEGLVTANEEGRALPTITTTPCVDGTVQLTGVEVRKPNGTQLINPLDL
                     RLEVGDTMVVTGQSGVGKTTLLRSLAELWPFTSGTLTRPCGPNETMFLSQMPYVPLGD
                     LRAVVSYPNEEGDIDDETLKRTLEKVALPHLVSRLDEVLDWAKVLSPGEQQRIAFARV
                     LLTKPKAVFLDEATSALDEGLEFLVYSLVRTELPDTILVSVSHRRTVEQHHTHELELL
                     GEGDWRFGQVQGDEKEPAPV"
     gene            complement(3801..>4799)
                     /locus_tag="VGP27_24020"
     CDS             complement(3801..>4799)
                     /locus_tag="VGP27_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7854399.1"
                     /translation="RGENVERRLLGGRLIEVMGNYGNWLNRMVRFTGWNLTVSQAINP
                     LPYIVQAPRLFAHEISFGDVIQSATAFVTIHNALSFFREAYDEFASYRAAVIRLDGLV
                     EENARARMFSQVTTANSVDGALDVEGVEVRTPDGANLIRALGLRLDPGDTLLVSGPSG
                     IGKTVLLQSLAGLWPFVTGSVRLPAGRQAAMFLPQLPYLPLGDLRAVASYPNEGTIGD
                     REIQQALIKVALPHLAIRLNDVGDWGKVLSVGEQQRIAFARILLTMPQAVFLDESTSA
                     LDEGLELTLYELIRVELPEMILVSVSHRATLEQFHARQLALVGGGEWRLEPLTARS"
BASE COUNT          947 a         1539 c         1535 g          778 t
ORIGIN      
        1 gtggctgacg ctgaccacga tgcagtccgg cagctgcgtg cgtagcgctc ggtagagcgc
       61 gaactcctga ccctcgtcga gcgcggacgt cgcctcgtcg aggaacaccg ctttcggttt
      121 cgccagcagc acacgggcga acgcgatgcg ttgttgctca cccggcgaga gcaccttcgc
      181 ccagtcggcc acctcgttga gacgggtgcc gaggtgggcg agcgagacgg cgtcgagcgc
      241 cgagttgacc gcgtcgtcgg gatacgagcc cgccccggcg ggataggaaa gcgtgctgcg
      301 taggtcgccc aacggcatgt agggaagctg cgacaggaac atggtgtcgt cctgcggacg
      361 gcgcagcgtg cccgacgtga acggccacag ctgagcgaga ctacgcagca gcgtggtctt
      421 accgctgccc gacgtcccac tgatcaccag cgactcgccg ggatcgagcc gaacgtcaag
      481 cgggtcgatg agccgtatac ccgcgggcgt gcgcacctcc acattgttca gctccagcga
      541 cccgtcgttg ctcggcagtg cggtcaaaga gggtagagct cttgccgttt cgttcgcagt
      601 gaccagaccg tggagacgga ttatggccgc gcggtagccg gcgaaggcgt catagaccgc
      661 gcggaagaac gacagcgagc cctgtagttg attgaaggcg ctcgacgact gcgtgacgtc
      721 gccgagctga atctgacccg cgaacaaccg cggcgcctga atgatcgtcg gcagcggatc
      781 gacgatctcg ttcatcgacc tgttccagcc gaggaacgcg atgccgcggc gaacgaaggc
      841 ccggtagttg gcgatgacgg ccgcgaaccg ggtcatcagc tcgccgcgtt cggcatcttc
      901 gccccgatac agtccgacgg cctcacccgc ctcgcggagc cggatgagcg cataccggaa
      961 cgcggcgttg gtgagttcgt tacggaacga cagccggatc agcgggcgac cgatccagaa
     1021 cgccaccacg gtcgtgagaa acacgtacaa cagcgcgatc cagaacagtg ccttgggcag
     1081 cgtcaaaccc agcaccgtca gcggaccgga cagcccccac aggatagggg tgaactcgac
     1141 gaccgagacg atcgcgaaaa tcgttccgaa caacaaggtc tgggccgtgc cgacggtcgg
     1201 cgtgttcgtt tccggtccgg tgccggtagt gaagatgtcg atgtcctgct gaatgcgttg
     1261 gtctgcattg tcgatcggct ggtcgatgaa ccgcccgcgg tagaaggcgt caccgtccag
     1321 ccagtcgccg gtcagccgat gggtgagcca cacccgccac ctgatgatga agcgccgcat
     1381 cagataggtg tcgagcagag ttcggacgac cttcgcgacg accaaggcgg ccaggatcag
     1441 tattgcgaac caaaagccat gtatcgcagc acctttcgca acaccttcgc cctcgaaggc
     1501 ggcctgcatc gccgagaact ggtcattgga ctgataggtg aagagcacaa cgaggcggac
     1561 gtcgatcatc acaaggacca agagcacgcc gagcagtccc cacaccgaaa tgctctggcg
     1621 acccgtgaag taatcgccgg tgacccgcca gaactgcctg ccccaggtgg tatgtcgcgc
     1681 cagcagcgcg cacacgacca ccatgccgat cgcggcgaac acccaggcct tggccaccca
     1741 cagcgccgac gcgacgagct cgtcgcccca gtcgagggac ggcgtgaacg tttccatgcc
     1801 gggaagctac cgcggcatcg tcacaccggt gcgggttctt tctcgtcgcc ctgaacctgg
     1861 ccgaatcgcc agtcaccctc gccgagcagt tcgagttcgt gcgtgtggtg ctgctcgacc
     1921 gtcctgcgat ggctgacgct gaccaggatg gtgtccggca gttcggtgcg caccaggctg
     1981 tagacgagga actcgagacc ctcgtcgagc gccgacgtcg cctcgtcgag gaaaacagct
     2041 ttgggtttgg tcaacagcac gcgggcgaac gcgatgcgct gctgctcgcc gggcgacagc
     2101 accttggccc agtcgagcac ctcgtcgagc cgcgagacga gatgcggtag cgccactttc
     2161 tccagcgtgc gcttgagcgt ctcgtcgtcg atgtcgcctt cttcgttcgg gtagctcacg
     2221 acggcacgca ggtctcccaa cggcacgtag ggcatctgcg acaggaacat cgtctcgttg
     2281 gggccgcacg gccgggtcag cgtgcccgat gtgaacggcc acagttcggc aaggctgcgc
     2341 agaagcgtcg tcttcccgac gcccgactgg ccggtgacga ccatggtgtc gcccacttcc
     2401 aggcgcagat cgagcggatt gatgagctgc gtgccgttgg gtttgcggac ctcgacacct
     2461 gtgagctgca ccgtgccgtc cacacagggt gtcgtcgtga tggtcggcag cgccctgccc
     2521 tcctcgttgg ccgtcaccag accttcgaga cgtatgatcg ctgcccggta tccggcaaac
     2581 aggtcgtagg cgttacggaa ccacgacaga ccggtctgga tggagccgaa cgccgacgcg
     2641 gactgcgaca tggcgccgag caggatgtca ccgctgtaga accgcgggaa ctgcagaagg
     2701 tagggcagcg gcacgatcag ctgacccatc gaccagttcc agccgttgaa accaagagac
     2761 cgcagcacgt aacgcttgta gttgtcgacg acgggccgga acagcttgcg cagcccggtg
     2821 cgctcggcga cctcgccgcg gtagaaggcc accgcctccg acgagtcgcg gagtcggacc
     2881 aaggcatacc ggaacgccgc gttgaacttc tcgttgctga acgacagcca gatgatcggc
     2941 cggccgaccc agaacgcgat cacggacgca aagagcacga agacgatgcc gatccagaac
     3001 atcgcgcgcg gaagcgagaa gtgaatcagt ggaagtgtga gaggtcctga cagaccccac
     3061 aggatcgcgg tgaacgaaac tatcgatgcg acggcgctga tcgcgccgaa cagaagggtg
     3121 ttctgcgacg agtaatacgg gacattgggc aacggcccgt tgttggccgt gaagatgtca
     3181 atgtcggatt ggatgcgctg gtccggattg tcgatcttgt cgtcgatgaa ccgagaccgg
     3241 tagtacgcct tgccgtcgag ccaatccccg gtgagccgat cggtgagcca ggctcgccac
     3301 gcgaggcaga accgttgcac catgaacatg tcgaccaaca gctgaaaaat gaaaagggcg
     3361 gcgagcacac agaaaacggc catcgagaaa tagaagccgt gcttacccga ttgcttgagc
     3421 gcttcattgt tggtgctgag acccccggcg acggcctgga aggcgttcga catgtcattg
     3481 ctgtagtagc tgagtagcac gtcgacgcgc acgccgagca tcacgacgaa cagaatcaga
     3541 ccgagccaca gccagacctt cacgctctcc gcgccgctga agtaggcgcc ggtgatgcgc
     3601 cagaactggc gcccccacac ggtgaagcgg ccgatgagga cgcagatcac caaggtcgcc
     3661 accgctgcaa tcgcccaggc tttcgcaagc cagatcagcg atacccagag ctcgcttccc
     3721 cagtcgagcg taggagccca catttccgat tccatgcggg caacgtacct cgccgagctt
     3781 gggccagcgc ttcaggcgcc tcaactgcgc gcggtgagcg gctccagccg ccattcgccg
     3841 ccgccgacga gtgcgagctg acgagcgtgg aactgctcca acgttgcccg gtggctcacg
     3901 ctgaccaaaa tcatctccgg tagctccacc cggatcagtt cgtacagcgt cagctccagg
     3961 ccctcgtcca gcgccgatgt cgactcgtcg aggaacaccg cctgcggcat ggtcagcaga
     4021 atgcgggcga acgcgatccg ctgttgctcg ccgaccgaga gcaccttgcc ccagtctccg
     4081 acgtcgttga gccggatggc caggtgcggc agagccacct tgatcagcgc ctgctggatc
     4141 tcgcggtcgc cgatcgttcc ctcgttgggg tatgacgcca cggcgcgcag gtcaccgagc
     4201 ggaagatacg gcagctgcgg aaggaacatg gccgcctgtc gtcctgcggg cagccgcacg
     4261 cttcctgtga cgaacggcca cagccccgcg aggctctgca gcagcacggt cttgccgatg
     4321 cccgacggtc cgctgaccag cagcgtgtcg cccgggtcga ggcgcaagcc aagcgcccga
     4381 atgagattcg caccgtcggg tgtgcgcacc tcgacaccct cgacgtccag tgctccgtcc
     4441 acagagttcg ccgtcgtcac ctggctgaac atgcgggcgc gcgcgttctc ctcgaccagt
     4501 ccgtcgaggc ggatcaccgc cgcccggtag ctcgcgaact cgtcgtaggc ctcccggaag
     4561 aacgacaacg cattgtgaat cgtgacgaac gcggtggccg actggatgac gtcaccgaac
     4621 gagatttcgt gggcgaaaag tcgtggcgcc tgcacgatgt agggcagggg gttgatcgcc
     4681 tggctcaccg tcaggttcca gccggtgaat cgcaccatgc ggttgagcca gttgccgtag
     4741 ttacccatca cctcgatgag tcgcccgccc agcagtcggc gttcgacgtt ctcgcctcg
//
LOCUS       DASYLX010001019         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1821914,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001019 DASYLX010000000
VERSION     DASYLX010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821914"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..662)
                     /locus_tag="VGP27_24025"
     CDS             complement(420..662)
                     /locus_tag="VGP27_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854400.1"
                     /translation="MNTHIARRVARCMALPVVAAGIIGGAALGLASTANAAEGTWSYD
                     PQVRSGIVAEPKTTAEPPAEAYPGAWWHRHNAWFLG"
     gene            995..1171
                     /locus_tag="VGP27_24030"
     CDS             995..1171
                     /locus_tag="VGP27_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854401.1"
                     /translation="MAVDGGAGVTVADSAVVGVVVVGVEVAVFDDEAAEVIGADGDAG
                     ATSLRCSRNVSRAG"
     gene            1303..1911
                     /locus_tag="VGP27_24035"
     CDS             1303..1911
                     /locus_tag="VGP27_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854402.1"
                     /translation="MIRRADSFQCAMEAANGLIDAAEATGNPFALSWALLAYGLAVHD
                     ADPVRSLAALRRGLVIAQDSGNSFVESTTAYTLGRDEAEHGDPLAAFDYFTVAIGNYH
                     DSGNTYMLRAPLGFLATFFDRLGRYEPAATIAGFVVSSPLAALPVMAEFGTAIAHLRN
                     VLGEATYESLARKGETMTTAEIVTYAYDQIDQTRAELNAVSK"
BASE COUNT          380 a          709 c          722 g          414 t
ORIGIN      
        1 gcggcgctga tgtcggtcgg cccagacggt gcctcgcgcc gcgcccgagc ccttgagatc
       61 ttcgatcgcg ttcccgaact cgaaacctgg acgtacggtc ccgaggccca ctgcgacgac
      121 gccgtccgcg cggaactact cgcttaagcc ttgagcccac gacgcgccgt gccctccttg
      181 ttcggcgagc ttcaggtgcg gcgaccgttg tcagatctag atgccgcggg ctgctggttg
      241 gggcctgcga acggcaatct ttagaagtgc gcgcttgcct gcccgagtcg ccgtcataca
      301 tgtcagtccc tgacggcgta ttccaagcat gcgaaccctg acccgattcg tcgccgcggt
      361 ggtggtgaag cctccccgca agttaggtcg gggagggcct caccgtaggt gcacgcacgc
      421 tatccgagga accaagcgtt atgccggtgc caccaggcgc cgggatatgc ctcggcaggt
      481 ggctccgccg tggttttcgg ctccgccaca atgcccgaac ggacctgagg gtcgtacgac
      541 caagtgccct cggcggcatt ggccgtgctg gccaggccca gggcagctcc gccgatgatc
      601 ccggcggcca caaccggcag cgccatgcag cgggcgacac ggcgagcaat gtgtgtgttc
      661 atttcgtgaa ttctcctgtc agagttgggt tttcatgtgt tgagtcggat tgttccggcc
      721 ctgcaataac tgtgccgccg cgaacccgct ttgtatgtcg ggcgaatggc gggtgaagcg
      781 gatgaattgg ttgtccaccg atcgggggaa gctgagaccg ccggtatgga gtgcggcgag
      841 gagcccggcg aggccgtaca cggcatgacg cgccagccaa accagttcgg cgggacggcg
      901 atggtggacc cacagcgcgc tgttttgccg ttatcaccga gtgccgcggg ttgcctgcag
      961 catccggcgg tggcggcgta tacggggcag accgatggct gtggacggtg gtgcgggcgt
     1021 gaccgtggcc gactccgctg tggtcggagt cgtcgttgtt ggtgtcgaag tggcagtgtt
     1081 cgacgatgaa gcggcagaag tcatcggggc ggacggcgat gctggcgcga cgtcgttgcg
     1141 ctgctcccgg aacgtcagca gggccgggtg atgtactggg cgacggctga ccggccgcac
     1201 tggtcgctga gcaggcgagc cgctcatgac ggcgcgaagt ttctctaccc gagcatccat
     1261 ttgtcgaccg gtcgccgtgc cggatggcgt ggtcgaaatc gcgtgatccg gcgcgcggat
     1321 tcgttccagt gcgcgatgga ggccgcgaac ggtctgatcg acgccgccga agcgaccggc
     1381 aacccgttcg cgctgtcgtg ggcgcttctg gcctacggtt tggccgtcca tgacgccgac
     1441 ccggtccgct cgctcgcggc cctgcgccgg ggcctggtga tcgcccaaga cagcgggaac
     1501 agcttcgtcg agtccacgac tgcgtacacc ctgggccgcg acgaggccga acacggggac
     1561 ccgctggctg ccttcgatta cttcaccgtg gcgatcggca actatcacga ctcgggcaac
     1621 acctacatgc tccgcgcccc cctggggttt cttgccacgt ttttcgaccg gctcggacga
     1681 tacgagccgg cggccaccat cgccgggttc gtcgtctcaa gccctctcgc tgcacttccc
     1741 gtcatggcag agttcggcac cgcgattgct caccttcgca atgtgctcgg tgaggcgacc
     1801 tacgaatcgc tcgcgcgcaa gggcgaaacg atgaccaccg cagagattgt gacgtacgca
     1861 tacgaccaaa tcgaccagac ccgagcagaa ctgaatgccg tctcgaaata gaccacattc
     1921 gagacgtccc agaccgacac ccacggcgta ggttctgcca ggtcgttgcg tctcaaaagt
     1981 tcgtctcaga agaacccatc gcaatattgc gacacagcgc tcatgcgaca tacgtttcgg
     2041 aatcagtgca ctgaagcgaa cttggagccc gtatccgata ctccgacaac gtgtccggtc
     2101 cgcttcccac ttcaaccgtc gtgttgccta actgcgtgca cagagccgta catcaggttg
     2161 gcatcagcat acctaggggc tcaacggacg ggccgcttcc ggtatcgcac gccgcgcgcg
     2221 atccg
//
LOCUS       DASYLX010001020         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1382171,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001020 DASYLX010000000
VERSION     DASYLX010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382171"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGP27_24040"
     CDS             <1..445
                     /locus_tag="VGP27_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEV7854403.1"
                     /translation="VLKVGPGLTFAMREALFALALIENELVPRPSRSNLIEVVERRML
                     ANPEYWQGYYESDPLTQRTARRYSYSDRLRYYWADDEVDAARRTLLQNLDRTGIPMPL
                     ISQFLPYQYDRIRAGDLEPSPQALVIDRIRDAMRPYARAVSGVTE"
     gene            442..1575
                     /locus_tag="VGP27_24045"
     CDS             442..1575
                     /locus_tag="VGP27_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV7854404.1"
                     /translation="MTDALPAQKDGGATILEIGQQPDVWREVAQSADGAVAEFLGAVT
                     SRLDIRIILTGAGSSAFIGDIAAPALRRHLGRRVEAIPTTSIVASPLDYLEPNTPTLM
                     VSFGRSGNSPESLATTALADELVDDIWHLVLTCDPDGELGRAHSGRANSRVVYMPERT
                     NDRGFAMTSSLTSMLLTSLLLLGPATADSGEALAAAAEHVATLQPDVAALAQTKKQRF
                     VYLGSGPLEGLAHESALKMLELTAGEAVTYFDSPLGFRHGPKSALDADTLVVVYVSTD
                     PYTRRYDLDIIAEIRAQLGQDAVAVISSEPIPETHGPALILPGLDGLDDALVALPYLV
                     FAQYLALFTALEHGKTPDNPFPSGEVNRVVQGVTIYPMAKSGE"
     gene            1578..2546
                     /locus_tag="VGP27_24050"
     CDS             1578..2546
                     /locus_tag="VGP27_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEV7854405.1"
                     /translation="MAFYLGVDGGGSKTALALIDDTGGVIARATAPTSYYFNDGFDVV
                     EKALAQGVTDICTQAAIAPADIEAAFFGLPGYGEASGDIEQLDAVPAGVLGHDRYSCD
                     NDMVCGWAGSLAGEDGINVISGTGSMTYGERQGVGHRVGGWGELFGDEGSAYWIATQG
                     LNAFSRMSDGRLPHGPLLQLIKDRLQVAGDLDVVSLVIDTWKGNRSKIAALATTVCEA
                     ARTGDEASARILSAAADELVTLIETTRTLVGFTDQETVPVSYSGGMFSDDGFRTLFVT
                     ALQGAPAKYDLQRPRLDPALGAALYAAKHSGHPLSPDAVQHLSDTN"
     gene            2562..3524
                     /locus_tag="VGP27_24055"
     CDS             2562..3524
                     /locus_tag="VGP27_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7854406.1"
                     /translation="MTETTAATGRTRSWIFYAALLIVFWGVWGAFSALPSTKYGYPDE
                     MIYSIWALTMIIPAAFILRGQRWDRRPAATIYGLIIGLTGAGGQLILFQALTMGPAYL
                     IFPIVSISPAITVIMAMVLLRERISPLAVVGLVLALAAIVMFTITGGESEGSHGPWLL
                     LAILICVAWGVQAYFMRKTATIGVNEATTFGWMTISGLLLIPVALISLGGIPTDFPWQ
                     APALTAITQVLNAVGALFLVMALARGKASIVAPTTNALAPALTVVISLIAYQTLPTPY
                     GAIGIVLALVGSTLMVYSDEKRGEALTEAVVADAPATTEHRPVQ"
BASE COUNT          583 a         1286 c         1090 g          575 t
ORIGIN      
        1 cgtcctgaag gtcggacccg gcctgacctt cgcgatgcgc gaagcactgt tcgcgttggc
       61 gctcatcgaa aacgagctcg taccgcggcc gtcgcggtcc aacctgatcg aggtcgtcga
      121 gcgcaggatg ctcgccaacc cggagtactg gcagggctac tacgagagcg acccgctcac
      181 ccagcgcacc gcccgtcgct acagctacag tgaccggctg cgctactact gggccgacga
      241 cgaggtcgac gccgcgcgcc ggaccctcct gcagaatctc gaccgcaccg gcatcccgat
      301 gccactcatc agccagttcc tgccctacca gtacgaccgg atccgagccg gtgacctcga
      361 gcccagcccg caggccctgg tcatcgaccg gatccgcgac gcgatgcgcc cctacgcccg
      421 agctgtgtct ggagtgaccg aatgaccgat gctctgcccg cccagaaaga cggcggcgca
      481 acgattctcg agatcggcca gcagcccgat gtctggcgcg aggtcgcaca gagcgccgat
      541 ggcgccgtcg ccgagttcct gggcgccgtc acctctcgcc tcgatatccg gatcatcctg
      601 accggggcgg gtagctccgc cttcatcggc gacatcgcgg cgcccgcgct gcggcgtcat
      661 ctcgggcgac gcgtcgaggc cataccgacc accagcatcg tcgccagccc gctcgactac
      721 ctggagccca ataccccgac actgatggtg tcgttcggcc gatcgggcaa cagcccggaa
      781 agcctcgcca ccaccgcact ggccgacgaa ctcgtcgacg acatctggca cctcgtcctc
      841 acctgcgacc ccgacggcga actcggccgg gcgcacagcg gccgggcgaa ctcgcgcgtg
      901 gtgtacatgc cggaacgcac caacgaccgc gggttcgcga tgacgtcgag cctcacctcg
      961 atgctgttga caagcctgct gctgctcggg ccggccactg ccgacagcgg tgaggcgctc
     1021 gccgccgcgg ccgagcacgt cgccactctt cagcccgacg tcgccgccct ggcgcagacc
     1081 aagaagcagc ggttcgtcta cctcggcagc ggaccgctgg aagggctcgc gcacgagtcc
     1141 gccttgaaga tgctcgaact gaccgccggt gaagcggtca cctacttcga ctccccgctg
     1201 gggttccggc acgggccgaa gtccgcgctc gacgccgaca cgctcgtcgt cgtctacgtg
     1261 tccacggacc cgtacacccg ccgctacgac ctcgacatca tcgccgagat ccgcgcccag
     1321 ctcggccagg acgcggtcgc ggtaatcagc agcgagccga tccccgaaac ccacggcccc
     1381 gctctgatat tgccgggcct cgacggtttg gacgacgctc ttgtcgcgct gccgtatctg
     1441 gtgttcgcgc aatacctcgc gctgttcacc gcgctggaac acggcaagac gccggacaac
     1501 ccctttccct ccggtgaggt caaccgcgtc gtgcagggcg tcaccatcta tccgatggcg
     1561 aagagcggag agtaggcatg gccttttatc tcggagtcga cggcggcggc tcgaagaccg
     1621 ccttggcgct catcgacgac accggaggcg tcatcgcccg cgcgaccgcg ccgacgtcct
     1681 actacttcaa cgacggcttc gatgtcgtcg aaaaggcgct cgcgcagggt gttacggata
     1741 tctgcaccca ggccgcaatc gcgccggcgg acatcgaggc ggccttcttc ggacttcccg
     1801 gctacggcga ggccagcggc gacatcgagc aactcgacgc cgtgcctgcc ggtgtgctcg
     1861 gccacgaccg gtacagctgc gacaacgaca tggtgtgcgg ctgggccggc tcgctcgccg
     1921 gcgaggacgg catcaacgtc atcagcggaa ccgggtcgat gacctacggc gagcgccagg
     1981 gcgtcggcca ccgggtcggc ggctggggtg agctcttcgg cgacgagggc tcggcgtatt
     2041 ggattgccac tcaagggctc aacgcgttct cccggatgag tgacggtagg ctgccgcacg
     2101 gtccgctgct tcaactcatc aaggaccgcc tgcaggtcgc cggtgacctg gacgtggtca
     2161 gcctggtcat cgacacgtgg aaaggcaacc gcagcaagat cgccgccctg gccaccacgg
     2221 tttgcgaagc cgcgcgcacc ggcgacgagg cgtcggcgcg catcctgtcc gcggcggccg
     2281 atgagctcgt caccctgatc gaaacgaccc gcacgctcgt cgggttcacc gaccaggaaa
     2341 ccgtcccggt gtcgtattcg ggcggaatgt tcagcgacga cggcttccgc accctgttcg
     2401 tcaccgcact gcagggcgca cccgccaagt acgacctgca acgcccgcgg ctcgacccgg
     2461 cgctgggcgc cgcgctgtac gccgccaagc acagcggaca tccgctgagt ccggatgccg
     2521 tgcaacatct ttccgacacc aactagacga ggtgaatcat cttgactgag accactgccg
     2581 ccacagggag aacccgcagc tggatcttct acgccgctct gctcatcgtg ttctggggcg
     2641 tctggggcgc gttctccgcc ttgccctcca cgaagtacgg ctatcccgac gagatgatct
     2701 acagcatctg ggcgctgacg atgatcatcc cggccgcgtt catactgcgc ggccaacgct
     2761 gggaccgtcg tcctgccgca acgatctacg gcctgatcat cgggctcacc ggtgccggcg
     2821 gccagttgat cttgttccag gcgctgacga tggggcccgc ctacctgatc ttcccgatcg
     2881 tctcgatctc ccctgcgatc accgtgatca tggcgatggt gcttctgcgc gagcggattt
     2941 caccgctggc cgtcgtcggc ctcgtcctgg cgctggcggc catcgtgatg ttcaccatca
     3001 ccggcggcga atccgagggt tcgcacgggc cttggcttct gctggcgatc ctgatctgcg
     3061 tggcgtgggg cgtgcaggcg tacttcatgc gcaagaccgc gacgatcggc gtcaacgaag
     3121 caaccacgtt cggatggatg accatcagcg ggctgctcct gatcccggtc gccctgatct
     3181 cgctcggcgg catcccgacc gacttcccct ggcaggcacc cgcgttgacg gcgatcacgc
     3241 aggtgctcaa cgccgtcggc gccctgttcc tcgtgatggc cctcgcccgc ggcaaggcct
     3301 ccatcgtggc gcccaccacc aacgccctcg cacccgcgct caccgtcgtc atctcgctga
     3361 tcgcctacca gaccctgccc actccctacg gcgcgatcgg catcgtgctc gcgcttgtcg
     3421 gctcgacgtt gatggtctac agcgacgaga agcgcggtga ggccctcacc gaggcggtcg
     3481 tcgccgacgc gcccgcaacc accgaacacc ggccggtgca gtgaaaccca ccgt
//
LOCUS       DASYLX010001021         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2589317,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001021 DASYLX010000000
VERSION     DASYLX010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589317"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VGP27_24060"
     CDS             <1..946
                     /locus_tag="VGP27_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7854407.1"
                     /translation="PAPQAPVDAAAAVAPAPPNPADPFAPPPAADPLAVPPADGAAPA
                     APPNPSAPAADPLAQPTTIPEGTPAGQNPTPYTGDPVFVPPSFNPLNGSMVGVGKPII
                     INFARPIADRPMAEQAIHISSNPPVPGKFYWMSATQVRWRPLNLWPANTTVNIDASGA
                     KSSFRTGESLVATVDDKTHQMTITRNGKVEKTFPVSMGKPDGKHETKNGTYYVLEKFA
                     SIVMDSATYGVPNTSPEGYKLTVQNAVRIDNSGSFVHSAPWSVGDQGKRDVSHGCINL
                     SPDNAKWFYDNFGSGDPVVIKNSAGTYNQNDGGQDWQI"
     gene            989..1912
                     /locus_tag="VGP27_24065"
     CDS             989..1912
                     /locus_tag="VGP27_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV7854408.1"
                     /translation="MAHRPRVVAVIVLLVVAAAALHGYLPGAETPPHDRPTTSFGSLF
                     AVVAMLVVSMAIIVIAVVTQSRHRPVSYGPGEPPREMTGQRRPLTWRMILFAVAVVIA
                     WLLLVALLSRMNAHLDVEQPPPDAGTPPSGPPRPPADQPQDPEPSSNVFSLLAGSTVL
                     LMMLVFVAAAIIGRRQRRRVTPVASSVDEYRPAAAVLGSDPLASAAEVGLAEAGDLSR
                     EPREAIIACYAAMERTLENSPDIVPRESDTPSEVLARAVEHHALRADSATELVDLFEE
                     ARFSPHVMTEDHRETAMRVLRLVLAELRSVA"
     gene            1915..2400
                     /locus_tag="VGP27_24070"
     CDS             1915..2400
                     /locus_tag="VGP27_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854409.1"
                     /translation="MKKLVAAGLCLVLGVQVLAMLMPDRRFVLVIVGVAVALTLLALR
                     GYLAHLSEEQPAAPMINDAEVSLRRWLSRTETLISWSESTRSDWDRHLRPMLARQFGI
                     ATGQKQNKNREGFRATGRMLFGDELWAWVDPENIARTDKHVPGPGREALDEILRRLEQ
                     V"
     gene            2397..3365
                     /locus_tag="VGP27_24075"
     CDS             2397..3365
                     /locus_tag="VGP27_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7854410.1"
                     /translation="MTSSSLPAATTTANCEAVLDEIARVVVGKRWALTLILTSVLARG
                     HVLIEDLPGLGKTLIARSFAAAFGLEFTRVQFTPDLLPADLLGSTIYDMQSGRFEFRS
                     GPIFTNLLLADEINRTPPKTQAALLEAMAEGQVSIDGVTHRLPDPFIVLATDNPIEYE
                     GTYPLPEAQLDRFAIRLELRYLSEQEETSMLRRRLERGSTEPTVNQVVDAHDLIAMRE
                     SVEQVTVHDDVLAYVVSLANATRQHPQVAVGASPRAELDLVQHARARALLLGRDYVIP
                     EDVKSLAIPTMAHRISLKPEMWVRRVHGADVVEELLRRLPVPRART"
BASE COUNT          551 a         1159 c         1137 g          545 t
ORIGIN      
        1 ccccgcgccg caggcgccgg tcgacgcggc cgcggcggtg gcaccggccc cgcccaatcc
       61 ggcggacccg tttgcgccgc cacccgcggc ggacccgctg gccgtgccgc cggctgacgg
      121 cgctgctccg gccgcgccgc cgaacccgtc cgcgcctgcc gccgatccgc tcgcgcagcc
      181 gacgaccatt ccggagggca ccccggccgg ccagaacccg acgccctaca cgggcgaccc
      241 ggtgttcgtt ccgccgtcgt tcaacccgct caacggctcc atggtcggcg tgggcaagcc
      301 gatcatcatc aacttcgcgc ggccgatcgc cgaccgcccg atggccgagc aggcaattca
      361 catctcctcc aacccgcccg tgcccggcaa gttctactgg atgagcgcca cccaggtgcg
      421 ctggcgtccg ctgaacctct ggccggcgaa tacgacggtc aacatcgatg cgagcggcgc
      481 caagtccagc ttccgcaccg gtgagtcgct ggtcgcgacg gtcgacgaca agacccacca
      541 gatgaccatc acccgcaacg gcaaggtgga gaagacgttc ccggtgtcga tgggcaagcc
      601 cgacggcaag cacgaaacca agaacggcac ctactacgtg ctggagaagt tcgccagcat
      661 cgtgatggac tccgccacct acggcgtgcc gaacacctcg cccgagggct acaagctgac
      721 cgttcagaac gcggtccgaa tcgacaacag cggctcgttc gtgcacagcg cgccgtggtc
      781 ggtcggcgat cagggcaagc gcgacgtcag ccacggctgc atcaacctca gccccgacaa
      841 cgccaaatgg ttctacgaca acttcggcag tggcgacccg gtcgtcatca agaactccgc
      901 aggcacgtac aaccagaacg acggcggcca ggactggcag atctgacgtt cgcttgcgac
      961 cttgatgccg gcgcggccag gatgaaatat ggcccatcgg ccccgcgtcg tcgccgtgat
     1021 cgtgcttctg gtcgtggccg ccgccgcgct gcacggatat ctgccagggg cggagacccc
     1081 gccgcacgac cggccgacga ctagtttcgg cagcctgttc gccgtcgtcg ccatgctggt
     1141 ggtgtcgatg gcgatcatcg tgatcgcggt cgtcacgcaa tcgcggcacc ggcccgtctc
     1201 atacggccct ggcgaaccgc cgcgcgagat gacgggccag cggcggccgc tgacatggcg
     1261 gatgatcctt ttcgctgtgg ctgtggtgat cgcctggctg ctgcttgttg cgctgctgtc
     1321 gcggatgaac gcgcacttgg acgtcgagca gccgccgcca gacgccggca cgccgccgtc
     1381 gggcccgccc cggcctccgg cggaccagcc gcaggaccct gagccgtcgt ccaacgtctt
     1441 ctctctgctc gccgggtcga cggtgctgct gatgatgctg gtgttcgtcg cggcggcgat
     1501 catcggccgc cggcagcggc gtcgtgtcac accggttgcg tcgtccgtcg acgaataccg
     1561 tcccgccgca gcggtattgg ggtccgatcc gcttgccagc gcggcggagg tcgggctggc
     1621 ggaggccggc gacctgagcc gcgagccacg ggaagcgatc atcgcatgct atgcggcgat
     1681 ggagcggaca ctggagaatt cgccggatat cgttccgcgc gaatcggata cgccgtcgga
     1741 agtgttggcc cgcgcggtcg aacaccacgc gctgcgcgcg gacagtgcga ccgaactggt
     1801 cgacctgttc gaagaagcgc ggttcagccc tcacgtgatg accgaggatc accgggagac
     1861 cgcaatgcga gtgctgaggc tcgtcctcgc cgaactgcgg agcgtcgcat gaacctgaag
     1921 aagcttgtcg ccgccggcct gtgcctggtg ttgggcgtgc aggtactggc catgctgatg
     1981 cccgaccgac ggttcgttct cgtcatcgtc ggggttgccg tggcgctgac attgctcgcc
     2041 ctgcgcggct atctggcgca cttgagcgaa gagcagcccg cggccccgat gatcaacgac
     2101 gcagaagtgt cgttgcggcg ctggttgtct cgaaccgaga cgctgatctc gtggtcggag
     2161 tccacccgat cggactggga tcggcacctg cgaccgatgc tggcgcggca gttcggcatc
     2221 gcgacgggcc agaagcagaa caagaaccgt gaagggttcc gcgccaccgg ccggatgctc
     2281 ttcggcgatg aactgtgggc gtgggtggat cccgagaaca tcgcgcgcac ggacaagcat
     2341 gtgcccggcc ccggccggga ggcgctcgat gaaatccttc ggaggctgga gcaggtatga
     2401 cgagttcttc actgccggcg gcgaccacca cggccaactg cgaagcggtg ctcgacgaga
     2461 ttgcgcgcgt cgtggtcggc aagcgatggg cgctgacgtt gatcctgacg tcggtgctcg
     2521 cgcgcgggca tgtgttgatc gaagaccttc ctggcctcgg caagacgctg atagcgcggt
     2581 cgtttgcggc ggcattcggc ctggagttca cccgtgtgca gttcaccccc gacctgcttc
     2641 cggccgacct gctcggttcg acgatctacg acatgcagtc gggccgtttc gaattccgct
     2701 cggggccgat attcacgaac ctgctgctgg ccgacgagat caaccggacg ccgccgaaga
     2761 cgcaggccgc gttgctcgag gcgatggccg aaggccaggt cagcatcgac ggcgtcaccc
     2821 accggctgcc cgacccgttc atcgtgctgg cgaccgacaa ccccatcgag tacgagggca
     2881 cctatccgct gcccgaggcg cagttggacc gcttcgcgat ccggctcgaa ctgcgctacc
     2941 tgtccgagca ggaggagacg tcgatgttgc gccgccgcct cgagcgcggc tcgacggagc
     3001 cgacggtcaa tcaggtggtg gacgcccatg atctgatcgc catgcgcgag tcggtggagc
     3061 aggtgaccgt gcacgacgat gtgctggcct acgtcgtgtc gctggccaac gccacccgtc
     3121 agcatccgca ggtggccgtc ggcgccagtc cgcgagccga actcgacctg gtgcagcacg
     3181 cgcgtgcgcg ggctctcctg ttgggtcgcg actacgtgat acccgaggac gtgaagtccc
     3241 tcgccattcc caccatggcc caccggatca gcctgaagcc ggagatgtgg gtgcgccgtg
     3301 tgcacggcgc cgacgtcgtg gaggagctgt tgcggcggct gccggttccg cgggcccgta
     3361 cgtgatcgag acccgtcgca ccgaggtcga ct
//
LOCUS       DASYLX010001022         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1053821,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001022 DASYLX010000000
VERSION     DASYLX010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053821"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VGP27_24080"
     CDS             complement(<1..773)
                     /locus_tag="VGP27_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7854411.1"
                     /translation="MSQVPNIELNDGTKIPQLGFGVYQIDPGETAKAVRTALDIGYRH
                     IDTAEMYQNEAGVGHGIRDAGIDRGEVYVTSKLNNGFHKPDDARRAFDGTLSALGFDY
                     VDLFLIHWPLPTLYDGDFVSTWQTLEEFKRDGRARSIGVSNFQVGHLERLAKETDTVP
                     AVNQIEVHPYFTNDEVRAYGRQHGIATEAWSPIAQGAVLDDQVVEGIAKATGKSPAQV
                     VLRWHIQRGDIVFPKSVTPERIKENFEIFDFELSPDDAEA"
     gene            988..1221
                     /locus_tag="VGP27_24085"
     CDS             988..1221
                     /locus_tag="VGP27_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854412.1"
                     /translation="MTKNTIARYIALPIVSAGIVGGAALGLAGMANAATPTVASGPGY
                     SYAPSVKAKPAPEAKPGWRNHKGIRHINALNGN"
     gene            1355..2194
                     /locus_tag="VGP27_24090"
     CDS             1355..2194
                     /locus_tag="VGP27_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854413.1"
                     /translation="MGFPPQHQEPPGLQSAMDPVPDCGENSYRGSGRLRGKRAIITGG
                     DSGIGRAAAIAYAREGADVLIAYLNEDADAKEVAQLVEDAGQRCVLVAGDLSDPAHCR
                     AVVERAAAEFGGIDILVSNAAYQMTHESLDEISDEEWDYTFRLNVGAYFHLVKAALPH
                     MSAGASVIGSSSVNSDMPSPTLAPYAATKAAIANLSASLAQLLGEKGIRVNSVAPGPI
                     WTPLIPSTMPEEKVASFGDDTPLGRAGQPAELAPVYVLLASDEGSYVSGARVAVTGGR
                     PVL"
     gene            complement(2191..>2676)
                     /gene="rsgA"
                     /locus_tag="VGP27_24095"
     CDS             complement(2191..>2676)
                     /gene="rsgA"
                     /locus_tag="VGP27_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HEV7854414.1"
                     /translation="GPFAAQFADLDLTIAIAGRDDPLDAVAPLLTDKVTVLLGHSGVG
                     KSTLVNRLVPEAERAIGDVTAVGKGRHTSTQSVALPLDGCGWVIDTPGIRSFGLAHIQ
                     PDDVMLAFSDLAEAIKDCPRGCGHLGPPADPECALDTLTGPAAGRVAAARRLLSALRE
                     A"
BASE COUNT          455 a          889 c          887 g          445 t
ORIGIN      
        1 gcctcggcgt cgtccggact cagctcgaag tcgaagatct cgaagttctc cttgatccgc
       61 tccggtgtca ccgacttcgg aaacacgatg tcaccacgct ggatgtgcca gcgcaacacg
      121 acctgggccg gcgacttgcc cgtcgccttg gcgatgccct ccacgacctg gtcgtcgagc
      181 accgcgccct gtgcgatggg ggaccaggcc tcggtggcga tgccgtgctg ccggccgtag
      241 gcgcgtacct cgtcgttggt gaaataggga tgtacctcta tctgattcac cgcgggcacg
      301 gtgtcggttt ccttggcaag ccgctcgagg tggccgacct ggaagttcga caccccgatg
      361 ctgcgggcgc ggccgtcgcg cttgaactcc tcgagcgtct gccacgtcga gacgaagtca
      421 ccgtcgtaga gcgtcggcag cggccagtgg atcagaaaca ggtcgacata gtcgaagccg
      481 agcgccgaca gcgtgccgtc gaaggcgcga cgggcgtcgt cgggcttgtg gaagccgttg
      541 ttgagcttgc tggtgacgta cacctcgccg cggtcgatcc ccgcgtcgcg gatgccatgc
      601 ccgacgcccg cctcgttttg atacatctcg gcggtgtcga tgtggcggta gccgatgtcc
      661 agcgccgtcc tgacggcctt ggcggtctcg ccgggatcga tctggtaaac gccgaagccc
      721 agctgcggga tcttcgtgcc gtcgttcaac tcgatattcg gtacttggct cacggttgtt
      781 cctcctcttc ggtcagcctg cccggtactg gaaaacgtca aacgcccgca tctgtgattt
      841 ttgtcctccg atcgggggac atgaagttcc accgaccgac cgcccgatcg cccgacatac
      901 agcgccgttg ttctccggca gtcttaagtc atcaccggaa cacaccggca gacaaaccaa
      961 aatccaactc acacaggaga attcgaaatg acgaagaaca cgattgcccg ctacatcgcc
     1021 ctcccaatcg tctcggcagg catcgtcggc ggagccgccc tcggcctggc gggtatggcc
     1081 aacgccgcga ccccgaccgt cgccagcggc cccggctact cctacgcccc gagcgtcaag
     1141 gccaagccgg cccccgaggc caagccgggc tggcgcaacc acaagggcat tcggcacatc
     1201 aacgccctga acggcaactg ataagccccc accgcaagac cctccccgaa tcgggggagg
     1261 gtcttgcggt ggggttgggc ccgtcatatc cgggtatgtg gcgacggaaa cggcgcacaa
     1321 cacgtcgacg gagcataggt aggaggtagt ggccatggga tttccaccgc agcaccagga
     1381 gccgccagga cttcagtcgg cgatggatcc ggtcccggat tgcggtgaga acagctatcg
     1441 gggttcgggc cggctgcgcg gtaagcgcgc catcatcacc gggggcgaca gcggtatcgg
     1501 acgtgcggcc gcgatcgcct acgcgcgtga gggcgccgat gtgctgatcg cctacctcaa
     1561 cgaggacgcc gacgccaagg aggtcgctca actcgttgag gatgcgggtc agcggtgtgt
     1621 gctggtggcc ggtgacctat ccgatcctgc gcactgccgg gcggtggtgg agcgagctgc
     1681 ggcggaattc ggcggcatcg acatcctggt cagcaacgcc gcctatcaga tgacccacga
     1741 gagtctcgac gagatcagcg acgaggaatg ggattacacc ttccggctga acgtcggcgc
     1801 ctatttccac ctcgtgaagg ccgcgctgcc gcacatgagc gccggggcgt cggtgatcgg
     1861 cagctcctcg gtcaactccg atatgccctc gcccacattg gcgccgtacg ccgccaccaa
     1921 ggcggccatt gcgaatctgt cggccagcct ggcgcagctg ttgggcgaga agggcattcg
     1981 ggtcaacagc gtcgcgcccg ggcccatctg gacgccgctg attccgtcga cgatgccgga
     2041 ggagaaggtc gcatcattcg gggacgacac gccgctgggt cgcgcaggcc agcccgccga
     2101 actcgcgccg gtctatgtac ttctggcctc cgacgaaggc agctacgtct cgggtgcgcg
     2161 ggtggctgtc accggcgggc ggccggtcct ctaggcctct ctcagcgccg acagcaggcg
     2221 cctcgccgcc gcgacgcggc ccgccgcggg gccggtcaac gtgtccagcg cgcattcggg
     2281 atcggcgggc gggccgaggt ggccacatcc acggggacag tccttgatcg cctcggccaa
     2341 atccgagaag gccagcatca cgtcgtcggg ctggatgtgg gccagtccga acgagcggat
     2401 gccgggcgtg tcgatgaccc agccgcagcc gtcgagcggc agcgccaccg actgcgtcga
     2461 cgtgtgccgc cccttgccga ccgcggtcac atcaccgatg gcccgttccg cctcgggcac
     2521 aagccgattc accagcgtgg actttccgac gccggagtgg ccgagcagca cggtcacctt
     2581 gtcggtgagc agtggggcga cggcgtcgag cggatcgtcg cggccggcga tcgcgatggt
     2641 cagatcgagg tcggcgaact gcgcggcgaa cggccc
//
LOCUS       DASYLX010001023         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2042559,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001023 DASYLX010000000
VERSION     DASYLX010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042559"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1160
                     /locus_tag="VGP27_24100"
     CDS             69..1160
                     /locus_tag="VGP27_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854415.1"
                     /translation="MARHASPEMTEMTKTGIRNMVLAMMALTLFVVAMIASYSGAFAR
                     PTLHHLTVAVSAPAQVVYAVRGQDALTVNVVGDDAAARQQVLDRAADSALVVAPTGQM
                     KIYVAGGGGKSVATAAEAVGRAIALKGRFTPVVEDVAPTSAADPSGTVEFYAVIFLSI
                     GASLGATAFGYIMGKVRRPGTFLLRTLTLSGYSALLAGVVTVYVDAMLGALPGHPWAV
                     FGALWVYSMAVGGAVTGAAAAFGSTASILLTLFLVVVGNAAAAGPVGRPLLSPFYATF
                     TMIVPQGSGVALLRSISYFGGHSAATPLLTLGVWGAAGCLLAALATASRVNYRALRER
                     IAEPARSVLRPRHLSPAGRHAVGAGADVG"
     gene            1063..>2690
                     /locus_tag="VGP27_24105"
     CDS             1063..>2690
                     /locus_tag="VGP27_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7854416.1"
                     /translation="MSVSLSQPDRYYDLGTYHRQVDTPSAQAQTWVDRGLVWAYAFNH
                     DEAIRCFERALELDPDLAIARWGIAYSVGPNYNKAWEAFDPVDLAASLARARMELGLA
                     STGRASAVERALIEALKGRFPTEDPDDIDALHAGHVSYADAMATLAEVYPDDVDVQAL
                     AADALVNVTAWALWDTRTGEPAPGSRVLEAKRILDDALSVAAGREHPGVLHLYLHTME
                     MSATPQDALPAADLLRGLVPDAGHLQHMASHIDVLCGDYRNSVVSNLTAVQADRLFVE
                     REGPLNFYSLYRAHNLHFVVYSAMFEGNSAIAMRAADELAEQLTPELLAIESPPMADW
                     LEAFVPLRIHVLVRFGRWDELISQPVPDDVELYCTTAATIHYGRGVAHAAKGQISQAH
                     AEREAFTAAYGRIPESRYLFNNTARDILAVAAEMLDGEIAYRKGEVDDAFAHLRRAID
                     LDDALPYDEPWGWMQPTRHAYGALLLEQGLVEQAAAVYAADLGLDPTLSRPCQHPGNV
                     WSLHGYHECLQRLGRTAEAGIIGQQLALAMARADV"
BASE COUNT          417 a          900 c          898 g          475 t
ORIGIN      
        1 gctgcttggc ctgtctgctt ccgccgcagt gcgctgaacc gattcgagct tccaagaagg
       61 gttttcacat ggcacgacat gcttctcccg agatgaccga gatgacgaag acgggcatcc
      121 gcaacatggt gctcgcgatg atggcgttga cgctgttcgt cgtcgcgatg atcgccagct
      181 actccggcgc cttcgccagg cccaccctgc accacctgac cgtcgcggtg tccgcgccgg
      241 cacaagtggt ctacgcggtc cgcggccagg atgctctgac ggtcaacgtg gtgggtgatg
      301 acgcggcggc gcggcaacag gtcctcgacc gagcggcgga ctccgccctc gtcgtcgccc
      361 cgacaggcca gatgaagatc tatgttgccg gcggcggtgg caagagcgtt gcgaccgcag
      421 ccgaagccgt tggccgggca atcgcgttga aggggcgttt caccccggtc gtggaggatg
      481 tggcgccgac gtctgcggcg gacccctcgg gcaccgtcga gttctacgcc gtcatcttcc
      541 tgtcgatcgg agcatcgttg ggcgcgacgg cattcggcta catcatgggc aaggtgcgca
      601 ggcccggcac cttccttttg cgcacgctga cgctgagcgg atattcggcg ctgctggccg
      661 gcgtggtgac ggtctacgtc gacgccatgc tcggcgcgtt gcccggacac ccgtgggccg
      721 tgttcggtgc gctgtgggtc tactcgatgg ccgtcggtgg cgcggtcacc ggcgccgcgg
      781 cggcattcgg atccacagcc tcgatattgc tgacgctgtt cctggtggtc gtcggcaacg
      841 cggccgccgc agggcccgta ggtaggccgc tgctgtcgcc gttctacgcc acgttcacga
      901 tgatcgtgcc gcagggttcc ggtgtcgcac ttctgcgcag catttcctac ttcggcggtc
      961 acagcgcggc gactccgctg ctgacgctcg gcgtgtgggg tgcggccggc tgcctgctcg
     1021 cggcgctcgc cacggcatca cgagtgaatt atcgtgccct tcgtgagcgt atcgctgagc
     1081 cagcccgatc ggtactacga cctcggcacc tatcaccggc aggtcgacac gccgtcggcg
     1141 caggcgcaga cgtgggttga ccgtgggctc gtatgggcgt acgcgttcaa tcacgacgaa
     1201 gcgatccgct gcttcgaacg cgccctcgaa ctggaccccg atctcgccat cgcccgctgg
     1261 ggcatcgcct actccgtcgg cccgaactac aacaaggcat gggaggcgtt cgatccggtc
     1321 gatctcgcag cgtcattggc gcgcgcgcga atggaactcg ggctggcgag caccggacgg
     1381 gcttccgctg tcgaacgcgc gctgatcgag gcgctgaagg gtcggtttcc gaccgaggac
     1441 cctgacgaca tcgacgcgtt gcatgccgga cacgtgagct acgccgacgc gatggcgaca
     1501 ctggccgagg tgtatcccga cgacgtcgac gtgcaggcgc ttgccgccga cgcgctggtc
     1561 aacgtcaccg catgggcgtt gtgggacacc agaacaggtg agcccgcacc gggctcacga
     1621 gttctggaag ccaagcgcat cctcgatgac gcgctttcgg ttgcggcggg ccgcgagcat
     1681 cccggtgtgc tgcacctcta tctgcacacc atggagatgt cggccacccc gcaggacgcc
     1741 ctgcctgccg ccgatctgct acgtggcctg gtcccggacg cgggtcatct gcagcacatg
     1801 gccagccaca ttgacgtgct gtgcggcgac taccgcaact cggtggtgtc caatctgact
     1861 gctgtacagg ccgatcgact tttcgtcgag cgcgagggac cgctgaattt ctattcgctc
     1921 taccgcgcgc acaatttgca cttcgtggtc tactcggcga tgttcgaggg caactcggcc
     1981 atcgcaatgc gtgccgccga cgagctggcc gaacagctca ctccggaact gctggccatc
     2041 gagtcgcctc cgatggcgga ctggctggaa gcgttcgtac cgttgcgaat ccacgtgctg
     2101 gtccggttcg gccggtggga cgagctgatc tcccagcctg tcccagacga cgtcgagctg
     2161 tactgcacga cggccgccac catccactac ggccgcgggg tcgcgcacgc tgcgaagggg
     2221 cagatctcgc aggcgcatgc ggagcgtgag gcgttcacgg ccgcttacgg ccgcattccc
     2281 gagtcccggt acctgttcaa caacaccgcg cgcgacatcc tggcggtcgc cgcggaaatg
     2341 ctggacggcg aaatcgctta ccggaaaggc gaagtcgacg acgcgttcgc gcacctgcga
     2401 cgcgcgatcg acctcgacga cgcgcttccc tacgacgaac cctggggctg gatgcaaccg
     2461 acccgccacg cgtacggggc actgctgttg gagcagggcc tggtcgagca ggccgccgcg
     2521 gtgtacgccg ccgatctcgg gctcgatccc accctcagcc ggccgtgcca gcaccccggc
     2581 aacgtgtgga gcctgcacgg ctatcacgaa tgcctgcaac ggctgggccg cacggcggag
     2641 gcgggcatca tcggccagca gctggcgctg gcgatggcgc gcgcggacgt
//
LOCUS       DASYLX010001024         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1822372,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001024 DASYLX010000000
VERSION     DASYLX010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822372"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1083
                     /locus_tag="VGP27_24110"
     CDS             58..1083
                     /locus_tag="VGP27_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV7854417.1"
                     /translation="MSDHIDTARLADWMDGADLPGGGEPLEADFLSGGTQNVIYRIRR
                     GEHDCVLRMPPPDAPPDRDKGILREWRIIDALDGTEVPHTAAVGVCPDASVLGRPFYL
                     MGFVDGWSPMDLDQRRWPEPFDSDLAARAGLAYQLAEGIALLSKVDWKAKGLQDLGRP
                     DGFHERQVDRWTGFFARIKGREIDGLDVATEWLRAHRPLDFIPGLMHGDYQFANVMYR
                     DGAPAQLAAIVDWEMGTVGDPKLDLGWMVQSWPEQTDAPSAMSYVDMRGMPSRKEVID
                     HYATVSGRQVDDLDYYLVLAKWKLAIVLEQGFQRAGDNEKLLAYGPIVVDLMRSAADL
                     AESSDYA"
     gene            1083..>1571
                     /locus_tag="VGP27_24115"
     CDS             1083..>1571
                     /locus_tag="VGP27_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV7854418.1"
                     /translation="MRAAVCPEYGPPEVIRIDERPSEALEAGQVRVQVGAAAVNYPDV
                     LLVANQYQISVPPPFVVGSEFAGVVVEVAEGTVDFEVGDRVTGTGMFGAFAEEVVVTG
                     QGLTRIPDAVDVRTAAAFGVAHRTAYHALRSVARVHAGDAVIVLGAGGGVGLAAVQLA
                     TAL"
BASE COUNT          262 a          505 c          553 g          251 t
ORIGIN      
        1 acgcgaagcg gcacaacaga agttcgccga gtacctggag catgaggtgg gaaacctgtg
       61 agcgatcaca ttgacaccgc gcgactcgcc gactggatgg acggcgcgga tctgcctggg
      121 ggcggcgagc ccctggaagc cgacttcctg tccggcggca cgcagaacgt gatctaccgc
      181 atccgccgcg gcgagcacga ctgcgtgctg cgcatgcctc cgccagacgc cccgcccgac
      241 cgggacaagg gcatcctgcg ggagtggcgg atcatcgatg cgctcgacgg caccgaggtg
      301 ccacacaccg ccgcggtcgg agtctgcccc gacgcctcgg ttctcggtcg ccccttttac
      361 ctgatgggct tcgtcgacgg ctggtcgccg atggacctcg accaacgacg gtggcccgaa
      421 ccgttcgaca gcgatctcgc cgcgcgcgca ggcctggcat accaactcgc cgagggcatc
      481 gcgctgttgt cgaaggtgga ctggaaggcc aagggactgc aggacctcgg cagaccggac
      541 ggcttccacg aacgccaggt ggaccggtgg accggattct tcgcgcggat caagggccgc
      601 gagatcgatg gcctggatgt ggcgaccgaa tggttgcgcg cgcatcggcc actcgacttc
      661 atacccggct tgatgcacgg cgactatcag ttcgccaacg tgatgtaccg cgatggtgcg
      721 cccgcccagt tggcagcgat cgtcgactgg gagatgggca ccgtcggcga cccgaagctg
      781 gatctcgggt ggatggtgca gagctggcct gagcagaccg atgcaccgtc ggcgatgagc
      841 tacgtcgaca tgcgcggaat gccctcccgc aaagaggtga tcgaccacta cgccacggtg
      901 tccggtcggc aggtcgatga cctcgactac tacctggtgt tggcgaagtg gaagctcgcc
      961 atcgtcctcg agcagggctt ccagcgcgcg ggcgacaacg agaaactgct ggcctatgga
     1021 ccaatcgtcg tcgacctgat gcgttcggcc gcagacctcg ccgagtccag cgactacgcg
     1081 tgatccgcgc cgcggtctgc cccgagtacg ggccgcccga agtcatccgg atcgatgagc
     1141 gcccttcgga agcgctcgag gccgggcagg tgagggtgca ggtcggcgct gcggcggtca
     1201 actatcccga cgtgctgctg gtcgcgaacc agtatcagat cagtgtgccg ccgccgttcg
     1261 tggtgggcag tgagttcgcg ggcgtcgtcg tcgaagtcgc cgaagggaca gtcgacttcg
     1321 aagttggtga ccgggtcacg ggaacgggga tgttcggcgc cttcgccgaa gaggtcgtgg
     1381 tgaccggcca gggattgacc cgaattccgg acgccgtcga cgttcgcacg gcggccgcct
     1441 tcggagttgc gcaccggacg gcgtatcacg cgctgcgctc ggtcgcgcgg gtgcacgcgg
     1501 gcgacgccgt gatcgtgctc ggcgcgggcg gcggggtcgg cctcgccgcc gttcaactag
     1561 ccaccgcgct g
//
LOCUS       DASYLX010001025         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3028917,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001025 DASYLX010000000
VERSION     DASYLX010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3028917"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /gene="murJ"
                     /locus_tag="VGP27_24120"
     CDS             complement(<1..1368)
                     /gene="murJ"
                     /locus_tag="VGP27_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV7854419.1"
                     /translation="MRGYPPPPPPRRMPPPPPRQRIPRGPAPRRRAGRPELSDAAVVS
                     RSWGMAVATLVSRITGFIRIVLLAAILGAALSSAFSVANQLPNLVAALVLEATFTAIF
                     VPVLARAERDDPDGGTAFVRRLVTLAVALLLVATVVSVVAAPLLVRLMLGSDPQVNRS
                     LTTAFAYLLLPQVIFYGLSSVFMAILNTRNVFGPPAWAPVVNNVVAIATLVLYLIVPG
                     ELSIDPVEMGNAKLLVLGVGTTLGVFAQTVVLLVAIRKERISLRPLWGVDDRLRRFGG
                     MAAAMVLYVLISQVGLIVGNQIASAAAASGAAIYNYTWLVLMLPFGMIGVTVLTVVMP
                     RLSRNAAADDTSAVLADLSLATRLTMVTLIPIVALMTVAGPAIGSALFAYGNFGDVDA
                     GYLGMAITLSAFTLIPYALVLLQLRVFYAREQPWTPIVVIIVITTVKIAASLAAPHLT
                     EDRELV"
     gene            complement(1479..>3251)
                     /locus_tag="VGP27_24125"
     CDS             complement(1479..>3251)
                     /locus_tag="VGP27_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854420.1"
                     /translation="KPVRTTVMWPLGDRPRLAPGAPGGSTPVRLTDDELATELAVGGR
                     LDVLLSAVDFATSPSVDPTGQVTSALCLAVDPDLLVTVNAMTAGYVVDDGPDAGPATP
                     THPGTGQEVALNWLTRLKAVAQRMCVAPANYAQADLDALQRVGDSMLSAVATNGAGDI
                     VDQILGITSVRGATLVGDGPLTEPAVQLLSAQGPTVAVAAANLNASDSQDGARATADV
                     SPVRYTPEVVVAPFDPTVSAALAGAGTDPWSPTYLDAALDIPVQHDSAVARRQDALGS
                     LLWRTIAPEAEPRTQLLMPPMEWGLQAEDAAAILSAVATSIRAGLAAPRPLAAVIAES
                     QAVPQQDSRPMPGNVAANPRARFDDDVISGIADTAGRLWGLTSALTTDERTGLTGTQY
                     TAPLREDMLRALSQSVPPEVRNGQAQQRLSVTSATVNDLFKAVTIVNPGGSYTLATEH
                     SPLPLALRNDLPVPIRVRLQVDAPPGMTVSDMGEIVLPPGYLPLQVPIEVHFTQRVAV
                     DVALRTIDGLPLGDPVRLSVHSNAYGKVLFFITLAVGAVLVALAGRRLWHRFRGQPDR
                     ADLDRPGRPDPLDTALAHSDGTEQ"
BASE COUNT          535 a         1097 c         1175 g          444 t
ORIGIN      
        1 caccaactcg cggtcttcgg tcagatgagg tgctgccagt gaggcggcga tcttcaccgt
       61 cgtgatgacg atgatcacca cgatcggcgt ccacggttgt tcgcgcgcgt agaacacccg
      121 aagctgtagc agcacaagcg cgtacgggat cagcgtgaac gccgacagcg tgatcgccat
      181 gcccaggtag cccgcgtcta cgtcgccgaa gttgccgtag gcgaacagcg cactgccgat
      241 cgccgggccc gcgacggtca tcaacgcgac gatgggaatc agcgtcacca tggtcagccg
      301 cgtcgccaac gacaggtcgg ccaacaccgc cgatgtgtcg tcggccgcgg cgttgcggct
      361 cagtcgcggc atcaccacgg tgagcaccgt cacgccgatc atcccgaacg ggagcatcag
      421 caccagccac gtgtagttgt agatcgcggc ccccgaagcg gctgcggcgc tggcaatctg
      481 gttcccgacg atgaggccga cctggctgat caacacgtag agcaccatcg ccgccgccat
      541 cccgccgaac cgccggaggc ggtcgtcgac accccacagc ggacgcaggc tgatccgttc
      601 cttgcggatc gccacgagca gcaccaccgt ctgcgcgaac acaccgagcg tggtgccgac
      661 gccgagcacc aatagcttgg cgttgcccat ctcgacggga tcgatcgaaa gctcacccgg
      721 cacaatcaga tacaggacga gggtggcgat tgcgacgacg ttgttgacaa ccggtgccca
      781 tgcgggcggg ccgaagacgt tgcgggtgtt caggattgcc atgaaaaccg acgacaggcc
      841 gtagaagatc acctggggaa gcagcagata cgcgaaggct gtggtcagcg accggttgac
      901 ctgcgggtcg ctgcccaaca tcagccggac cagcagtgga gctgccacca ccgacaccac
      961 cgtcgcgacc aacagcagcg cgacggccag cgtgacgaga cggcggacga aggcggtgcc
     1021 gccgtcggga tcgtcgcgtt cggcgcgggc cagcaccggg acgaagatcg cggtgaacgt
     1081 cgcttccagc accagcgccg cgacgaggtt gggcagctgg ttggccaccg agaaggcact
     1141 ggacaacgcc gcgccgagga tggcggcgag caggacgatg cggatgaacc cggtgatccg
     1201 gctgaccagc gtcgcgaccg ccatgcccca cgaccgcgac accaccgcgg cgtcgctgag
     1261 ctcgggccta ccggcccgcc gccgcggtgc cggtccccgc gggatccgct gtctgggcgg
     1321 tggtggcggc atccgccggg gcggtggcgg cggaggatag ccgcgcatgc cgggcggcgg
     1381 tggcggcacg gttcggggag gcagcggcgg tcggcccatg ggaccaccgc gccggtcgaa
     1441 gggtggctgc gccggtccga agtggcggcc ggtggtgctc attgctcggt tccgtcgctg
     1501 tgcgcgagcg cggtgtcgag cggatccgga cggcccggcc ggtccaggtc cgcacgatcg
     1561 ggctgaccgc ggaaccggtg ccacagccgg cgaccggcca gcgcgacgag aaccgcgccg
     1621 accgcgagcg tgatgaagaa cagcaccttt ccgtaggcgt tggagtgcac cgacagtcgc
     1681 accggatcgc cgagcggcag cccgtcgatg gtgcgcaagg cgacgtcgac ggcgacgcgc
     1741 tgggtgaagt gcacttcgat gggcacctgc aacggcaggt atccgggcgg cagcacgatc
     1801 tcgcccatgt cgctgaccgt catgccgggc ggagcgtcga cctgcaaccg cacccgaatc
     1861 ggcaccggca ggtcattgcg aagggccaag ggcagcgggc tgtgttcggt ggccagggtg
     1921 taggaaccac cggggttgac gatcgtcacc gccttgaaca ggtcgttgac cgtcgcgctg
     1981 gtgacagaca accgctgctg ggcctgcccg ttgcgaacct cgggcggcac cgactggctc
     2041 agcgcacgca gcatgtcctc ccgcagcggc gcggtgtact gcgtgccggt gagcccggtg
     2101 cgctcgtcgg tcgtcagcgc cgacgtcaat ccccacagcc ggcctgcggt gtccgcgatg
     2161 ccggagatga cgtcgtcgtc gaagcgggca cgcggattgg cggccacgtt gccgggcatc
     2221 ggacgggagt cctgttgcgg caccgcctgg ctctcggcga tcaccgccgc cagcggacgg
     2281 ggcgcggcaa ggcctgcccg gatggaggtg gccaccgcgg acaggatcgc cgcggcgtcc
     2341 tcggcctgca gaccccactc catcggcggc atcaggagct gggtgcgagg ctcggcctcc
     2401 ggggcgatgg tccgccacag cagagagccc agcgcatcct gcctgcgcgc caccgccgaa
     2461 tcatgctgca ccggaatatc aagggcggca tcgagatacg tgggtgacca cggatccgtg
     2521 cccgcccccg ccaacgcggc ggacacggtg gggtcgaacg gcgcgaccac cacttccggg
     2581 gtgtagcgaa ctgggctcac gtcggcggtg gcccgggcgc cgtcctgcga atcggatgcg
     2641 ttgaggttgg ccgccgcgac ggcgaccgtc ggtccctgtg cggagagcag ctgcaccgca
     2701 ggctcggtca gcggaccgtc gccgaccagg gtggcgccgc ggaccgaggt gatgccgagg
     2761 atctggtcga cgatatcgcc cgcgccgttg gtggcgacgg cgctgagcat ggagtcaccc
     2821 acccgctgca gggcgtcgag gtcggcctgc gcgtagttcg cgggcgccac gcacatccgc
     2881 tgggccaccg ccttgagccg ggtgagccaa ttcagcgcga cttcctggcc ggtgcccgga
     2941 tgcgtcggcg tggcaggtcc cgcatcgggt ccgtcgtcga cgacatatcc ggcggtcatc
     3001 gcgttgacgg tgaccagcag gtcggggtcg acggccagac acagcgcgct ggtcacctga
     3061 ccggtgggat cgacggacgg gctggtcgcg aagtcgaccg ccgacaacag cacgtcgagc
     3121 cgcccgccga ccgccagttc ggtggcgagt tcgtcgtcgg tcagccgcac cggtgtcgag
     3181 ccgccgggcg ccccgggcgc cagccgtggc cggtcgccga gcggccacat cacggtcgtg
     3241 cggacgggtt t
//
LOCUS       DASYLX010001026         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2809152,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001026 DASYLX010000000
VERSION     DASYLX010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2809152"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGP27_24130"
     CDS             <1..229
                     /locus_tag="VGP27_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7854421.1"
                     /translation="VSTAPMTVSVSFHEPVRWDGWILYTHESTQVGSGMSYVRGAVHT
                     EEGELIASFTQDALIRPLRTTDTSIKEQSRL"
     gene            323..901
                     /locus_tag="VGP27_24135"
     CDS             323..901
                     /locus_tag="VGP27_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854422.1"
                     /translation="MSPQTYRTLKRTDPRSQSAPRSPARAGTVLRHEMTVVASSVADV
                     VTSAGGWLYDRRMAGWQVNVLVADRTGARALQILGAQVLDLGGDLEAITDDPDRAATV
                     AVAADVYSTDERVRRFVTTSDRRRAEVALWGDAGSLGENVSAVSYRLTAAARVFKAQA
                     LLAAGIGDEPVSATEPLFRCGVTALAPVAARS"
     gene            complement(891..1715)
                     /locus_tag="VGP27_24140"
     CDS             complement(891..1715)
                     /locus_tag="VGP27_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854423.1"
                     /translation="MAEHKPLAGKVAYVTGAARGQGRSHCVRLARAGADIIAIDACGP
                     VAEHNGYPPAEPADLAETVNLVEGEGRKVRAEEVDIRDLAGQQRVVSGAIEQFGRLDI
                     VVANAGVLNWGRLWEISAQQWQDVIDVNLTGLWNTVKAVVPPMIEAGNGGSIITISSA
                     AGVKAVPGCGHYCASKFGVVGLTNSLAVELGEFGIRVNSVHPYGTDTPMGNDASMWQM
                     FADHPNFIHSFSPGALPTDSLAAPDLVSEIVVWLASDASSLVTAAQIPAEKGYLKI"
     gene            complement(1708..2226)
                     /locus_tag="VGP27_24145"
     CDS             complement(1708..2226)
                     /locus_tag="VGP27_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7854424.1"
                     /translation="MIEPDTKDWTWVITQPCPECGFDGATVSHTDVAGRIRADAAQWP
                     HRLSAANATVRPLPTVWSALEYGCHIRDVHRIFNDRVRLMLSEDDPRFANWDQDATAI
                     EDDYGSQDPVVVTSQLVHAAGTVADTYEHMPPDAWSRRGLRSNGSQFTVASIAVYHLH
                     DIVHHAHDVDHG"
BASE COUNT          386 a          794 c          801 g          399 t
ORIGIN      
        1 ggtgtcgacc gcaccgatga cggtgtcggt ctcctttcac gaaccggtcc gctgggacgg
       61 ctggatcctt tacacgcacg agagcacgca ggtcggctct ggcatgtcct atgtccgcgg
      121 cgccgtgcac accgaggagg gtgagctcat cgcgtcgttc acccaggacg cgctgatccg
      181 gccactgcgt accacggaca cctccatcaa ggagcagtcg cggctgtaac acgggcaaag
      241 cggaccttta catattctcg taggaatgtc acgctgtggt gagtcgcagc ccggacaccc
      301 gctcgtcgag aatggacccg ctatgtcgcc gcagacttac cgcacgctca agcgcaccga
      361 tccgagatcg cagtcggcgc cacggtcgcc ggcgcgcgcc ggcacggtgt tgcggcacga
      421 gatgaccgtc gttgcatcca gcgtcgccga cgtcgtgacg tcggcaggcg gttggctgta
      481 cgaccgaagg atggccggct ggcaggtgaa cgttctggtc gccgaccgca ccggtgcacg
      541 cgccctgcag attctcggag cgcaggtgct ggacctgggc ggagacctcg aggcgatcac
      601 cgacgatccc gaccgggccg ccaccgtcgc ggtcgccgcc gacgtgtact ccaccgacga
      661 acgggtccgg cgcttcgtta ccacctcgga ccgccgtcgc gccgaagtgg cgctgtgggg
      721 cgacgccggg tcgctcggcg agaacgtcag cgcggtctcc taccggctga ccgccgccgc
      781 aagggtattc aaggcccagg cgttgttggc cgcaggcatc ggggacgaac cggtctcggc
      841 gacggaaccg ctgttccggt gcggtgtcac cgccctggca cccgtggccg ctagatcttg
      901 agatagccct tctccgcggg gatttgcgcc gcggtcacca gtgacgaggc gtcgctcgca
      961 agccatacca cgatttcgga aacgagatcc ggtgctgcca gcgagtcggt gggcagcgcg
     1021 ccgggcgaga agctgtggat gaagttcgga tggtcggcga acatctgcca catcgacgca
     1081 tcgttgccca tcggggtgtc ggtgccgtac gggtgcaccg agttgacccg aatcccgaac
     1141 tcgcccaact cgaccgcgag cgagttggtc agcccgacga caccgaactt gctggcgcag
     1201 tagtgcccgc agccgggcac ggccttgaca cccgcagcgg agctgatcgt gatgatggac
     1261 ccgccgttgc ccgcctcgat catcggcgga accaccgctt tgacggtgtt ccacaacccg
     1321 gtcaggttga cgtcgatgac gtcttgccac tgctgcgctg aaatctccca cagccgaccc
     1381 caattgagca caccggcgtt ggcgaccacg atgtcgagac ggccgaactg ttcgatggcg
     1441 ccggaaacca cccgctgctg gccggcgaga tcgcggatgt cgacctcctc tgcgcgaacc
     1501 ttgcggcctt cgccctcgac caggttcacc gtctcggcga ggtcggccgg ttcggcgggc
     1561 gggtagccgt tgtgctcggc gaccgggccg caggcgtcga tcgcgatgat gtcggcgcct
     1621 gcacgggcga gccgtacgca atgagaacgt ccctgcccgc gggcggcacc ggtcacatag
     1681 gcgaccttcc ccgccagcgg cttgtgttca gccatggtcg acatcgtgcg cgtggtgaac
     1741 gatgtcgtgc aagtggtaga ccgcgatcga cgcgaccgtg aactgactgc cgttgcttcg
     1801 taacccgcgc cgcgaccatg cgtctggcgg catgtgctca taggtgtcgg ccaccgtgcc
     1861 ggccgcatgg acgagttggg acgtgacgac gacgggatcc tgagagccgt agtcgtcctc
     1921 gatggccgtc gcatcctggt cccagttggc gaagcgtgga tcgtcctcgc tgagcatgag
     1981 ccggacgcgg tcgttgaaga tccggtgcac gtcgcgaatg tggcagccgt actccagcgc
     2041 cgaccacacc gtcggcagtg gccggacggt ggcattggct gcgctgagcc ggtgcggcca
     2101 ttgggcggcg tcggcacgaa tgcggcctgc gacatcggtg tggctcaccg tggccccgtc
     2161 gaatccgcac tcgggacacg gctgcgtgat gacccaggtc cagtccttgg tatcgggctc
     2221 gatcatggcg tcagtgtggc agccgcctca cggatccgat cacccgcgcc ggtgtcgatg
     2281 gggtcgccgt gcccgaagca cgccgtcgca acgtccagcg ccgccaagcg cttgaacgat
     2341 gcgaccgcgc gcgcccgatc ctggttgaaa accccgagca
//
LOCUS       DASYLX010001027         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_945680,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001027 DASYLX010000000
VERSION     DASYLX010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945680"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGP27_24150"
     CDS             <1..252
                     /locus_tag="VGP27_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV7854425.1"
                     /translation="DLGLVYGFNVEEGDAGKVALIDMTLTSAACPLTDVIEDQSRTAL
                     VGAGLVNEIKINWVWNPPWGPDKITEDGREQLRALGFTV"
     gene            complement(249..1802)
                     /locus_tag="VGP27_24155"
     CDS             complement(249..1802)
                     /locus_tag="VGP27_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854426.1"
                     /translation="MSSGTDVNIVTKQGRSGRTMPFDMSGVVRTDDGVLRYTTLAPSL
                     LDMLRVSVDRHSDRECVVVLGGERVTYRQLWDRSARVAGGLRAAGVQPGDRVANRHGN
                     GLEWCLGFWGTLMAGAVVVPVNTRFSESEASYVVDDAGAAVVLQANSPLPDDEPYVRD
                     GAHHQTLAALMYTSGTTGFPKGAMVTHENFLSNVETCVRAMETPATTHIRNLISVPLF
                     HATGCNSQFLPTIHLGGTAVIMPAFDVQAFLRATADEKINVLTSVPAIFWLAMNQPDF
                     ADFDMSNVGWVTYGGAPTAPDVIARLRSSFPNARLANGFGLTETSSVSTLLPDEYAEL
                     RPETVGFAAPPVEVDLIDPDPVTGVGELLIRGQNIVAGYWNNPAKTAETFIDGWLHTG
                     DLARIDDDGFVQIVDRLKDMICRGGENVYCVEVENVLTQHPGIFEAAVLAVPDEMMGE
                     KVGAVVVPLPGTAPDPEDIRAFVSDRIADYNAPQFVCFRSEPLPRNPAGKVMKPTLRN
                     DTQWGDELR"
BASE COUNT          327 a          599 c          617 g          338 t
ORIGIN      
        1 gacctcggcc tggtatacgg cttcaacgtg gaagagggcg acgcaggaaa ggtggcgctc
       61 atcgacatga cgttgacgtc ggccgcgtgc ccgctcaccg acgtcatcga ggatcagtcg
      121 cgcactgcgc tggtgggcgc cgggttggtc aacgagatca agatcaactg ggtgtggaac
      181 ccgccgtggg gtcccgacaa gatcaccgag gacggccgcg aacagctgcg cgcccttggc
      241 ttcaccgtct aacggagctc gtcgccccac tgagtgtcat tgcggagagt gggtttcatt
      301 accttgcccg ccgggttacg cggtagtggc tcgctgcgga agcacacgaa ctgcggcgcg
      361 ttgtagtcgg cgatgcggtc ggagacgaac gcgcgaatgt cctcggggtc cggcgccgtt
      421 cccggtaggg gcaccaccac cgcaccgact ttctcgccca tcatctcgtc gggcactgcg
      481 aggacggccg cttcgaatat gccggggtgc tgggtgagta cgttctcgac ctcgacgcag
      541 tacacgttct cgccgcctcg gcagatcatg tccttcaacc ggtcgacgat ctgcacgaag
      601 ccgtcgtcgt cgatgcgcgc gaggtcgccc gtgtgcaacc agccgtcgat gaacgtctcc
      661 gccgtcttgg ccgggttgtt ccagtagccc gcgacgatgt tctggccgcg gatgagcagt
      721 tcgccgacac ctgtcaccgg gtcgggatcg atgaggtcga cttcgacggg cggcgcggcg
      781 aagccgactg tctccggccg caactcggca tactcatcgg gcagcaaggt cgacaccgac
      841 gacgtctcgg tgaggccgaa accgttggcc agccgggcat tcgggaacga cgaccgaagg
      901 cgtgcgatga cgtccggtgc tgtcggcgct ccgccgtagg tgacccaccc gacgttgctc
      961 atgtcgaaat cggcgaagtc aggctggttc atcgccagcc agaagatcgc cggcaccgac
     1021 gtcaacacat tgatcttctc gtcagccgtc gcccgcaaga aggcctgcac gtcgaacgca
     1081 ggcatgatca ccgcggtgcc gccgaggtgg atcgtgggaa ggaactgact gttgcagccc
     1141 gtggcatgga aaagcggcac cgagatcagg ttgcgaatgt gggttgtcgc gggagtttcc
     1201 atggcccgaa cgcacgtctc cacgttcgac aggaagttct cgtgggtgac cattgcaccc
     1261 ttcggaaatc cggtcgtgcc gctggtgtac atcagcgcgg caagggtttg gtggtgggcg
     1321 ccgtctctca catacggctc gtcgtcgggt agtggcgagt tggcttggag gacgaccgcg
     1381 gcgcccgcgt cgtcgaccac gtaggacgcc tcgctctccg agaatcgggt gttgacgggc
     1441 accaccaccg ccccggccat cagcgtgccc cagaatccga ggcaccattc cagtccgttg
     1501 ccgtgccggt tcgcgacccg atcccctggt tgcacgcctg cggcgcgcag gccacccgcg
     1561 acgcgggccg agcggtccca caactgccgg taggtcaccc gttcgccacc gagaacaacg
     1621 acgcactcgc ggtcggaatg acgatccacc gacacgcgga gcatgtccag cagcgatgga
     1681 gccaacgtcg tgtagcgcaa gacaccgtcg tcggtacgga ccacgccgga catgtcgaac
     1741 ggcatcgttc gcccgctgcg gccctgcttg gtgacgatgt tgacgtcagt gcccgatgac
     1801 atgtccgtca gtgtggcgtg ttggccgttt taatctgccc caacccgaca aaattggttc
     1861 atggtgacgg tgcctgcgtt c
//
LOCUS       DASYLX010001028         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1054315,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001028 DASYLX010000000
VERSION     DASYLX010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054315"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VGP27_24160"
     CDS             complement(<1..355)
                     /locus_tag="VGP27_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7854427.1"
                     /translation="MWNPDVYLTFADHRGRPFFDLTSRVAAENPRRVVDLGCGPGNLT
                     VTLAERWPNAVIEAWDSSPEMVESAREWGVDAQTGDVRSWEPKPDTDVVVSNATLQWV
                     PEHVELLVRWAGALDA"
     gene            446..2281
                     /locus_tag="VGP27_24165"
     CDS             446..2281
                     /locus_tag="VGP27_24165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7854428.1"
                     /translation="MTANVDRRMQLVLAALLVATAALYLLNLSATGYGNLFYAAAAEA
                     GSHSWSAWFFGSLDANNFITVDKPPASLWVTALSVRLFGMNSWAVLAPQALMGAASVA
                     VLYATVRRGFDDARTGTAAGLLAGAGLACTPAAALMFRFNNPDALLVLVLTLAGYCLI
                     RAVESASWRWLALLGVAMGLAFLTKMGEGFLPLPAFALSYLLFAPTSLRNRLVHLLGA
                     AAALVAAAGWWVLTVQLVPATARPYIGGSTDNTVMQLAVGYNGMTRILGRDRNRAANL
                     AVPANGHGWSRLGGGTGLRRLFTAEMANEISWLLPAALLAIVFGCYLLARGRLDRGEK
                     ALLTMFAGWLLGSALVFSYMSGMVHPYYTVAMAPAVAGLVGIGAAWGWQDRSGWDGRV
                     ALAAMIVLTAVWSGILLHRNHFGPAWLPWTVAAVAIAAAAVLALGAHRIVTAATAVGV
                     LAAVGGPAAFSVATAATPHQGAIPTALQKGVFQLGNWTGDEASNPDLAGVLANTHTEW
                     SAATNGSQSAAALEVSSGTPVMAIGGWSGDPVPTLQTFIDDVHNGKISYYVEAGRGGA
                     AGQADRGEVIYGRSRGAAHTREIAEWVINHFQGTVIGESTVYRLT"
     gene            2292..>2690
                     /locus_tag="VGP27_24170"
     CDS             2292..>2690
                     /locus_tag="VGP27_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854429.1"
                     /translation="MSSTQPPDAAPADPPIPVPDVPGADAGARGLPRRVDLTLRQRLI
                     VDTSAIADLGLRTALASVVGAAMVPQIALAALNGSNAKEQRDALRFYAELGAVKDAGL
                     SFPTPTEVPRISSRPANPIAEWTANGRVHNI"
BASE COUNT          385 a          948 c          906 g          451 t
ORIGIN      
        1 ccgcatccag cgcccccgcc catcgcacga gcaactcgac gtgctcgggc acccactgca
       61 gcgtcgcgtt gctcaccacg acgtcggtgt cgggcttcgg ttcccaggac cggacatcgc
      121 cggtctgcgc gtccacaccc cactccctgg ccgactcgac catctccggc gagctgtccc
      181 acgcctcgat caccgcgttc ggccagcgtt cggcgagggt gacggtgaga ttgcccggac
      241 cgcaacccag gtcgaccacc cgtcgcggat tctcggcggc cacccgcgac gtcagatcga
      301 agaagggcct gccgcggtgg tcggcgaagg tcaggtagac atccggattc cacatgcgac
      361 acagtgtcgc attcggccgg ttcacaggtg cttcgcagcg gcggcccagc cccgcggcca
      421 gcaacgcact gcacggtgtt cttccatgac cgccaacgtg gacaggcgca tgcagcttgt
      481 gctcgccgcg ctgctggtag ccaccgcggc gctatatctg ttgaatctgt cggcaacggg
      541 ctacggcaac ctgttctacg ccgcggcggc ggaggccggt tcgcatagtt ggtcggcgtg
      601 gttcttcgga tcgctcgacg cgaacaactt catcaccgtc gacaagccgc ccgcctcgtt
      661 gtgggtgacc gcgctgtcgg tccggttgtt cggcatgaac agctgggcgg tgctggcacc
      721 ccaggcgctg atgggcgcgg cgtcggtcgc cgtcctgtac gccacggtgc gccgggggtt
      781 cgacgacgcg aggacgggta cggcggccgg actcctggcc ggtgcaggcc tggcctgcac
      841 acccgccgcg gcgttgatgt tccgattcaa caatcccgac gcactgctgg ttctggtgtt
      901 gaccctggcg ggttactgcc tgatccgcgc cgtcgaatcg gcctcctggc gctggctcgc
      961 gctgctcggt gtcgcgatgg gtttggcgtt tctcaccaag atgggtgaag gcttccttcc
     1021 gctacccgcg ttcgctctgt catatctgtt gttcgcaccg acgagcctgc gtaaccggct
     1081 ggtgcatctg ctcggcgccg cggcggcgct ggtcgcggcg gccggctggt gggtactgac
     1141 ggttcagctg gttcccgcga cagcgcggcc ctacatcggc ggatcgacgg acaacaccgt
     1201 gatgcagctt gccgtcgggt acaacggcat gacccgcatc ctcggccgcg accgaaaccg
     1261 cgcggccaac cttgcggtgc ctgcgaacgg gcacggatgg tcgcgactgg gcggcggcac
     1321 cggactgcgt cggctcttca ccgccgagat ggccaatgag atctcctggc tgttgcctgc
     1381 cgcgctgctg gccatcgtct tcggttgcta cctgctcgcc cgcggccggc tggaccgcgg
     1441 cgagaaggct ttgctgacga tgtttgccgg ctggctgctg ggcagcgcgc tggtgttcag
     1501 ctatatgagt ggcatggtgc acccctacta caccgtggcg atggcacccg cggtcgcggg
     1561 cctcgtcggc atcggcgcgg catgggggtg gcaagaccgg tcgggctggg acggccgcgt
     1621 cgcgctcgcc gcgatgatcg tgctcaccgc agtctggtcg gggatactgt tgcaccgcaa
     1681 ccacttcggt ccggcctggc tgccctggac cgtcgctgcg gttgcgatag ccgccgccgc
     1741 ggtgctcgcc ctcggcgcac accggatcgt cacagcggcc accgcggtgg gcgtgctggc
     1801 cgcggtcgga ggtccggcgg cgttctcggt cgccaccgcc gccaccccgc accagggcgc
     1861 gattccgaca gcactgcaga agggcgtctt ccagctcggc aattggaccg gtgacgaagc
     1921 ctccaatccc gacctcgccg gagtgctggc caatacgcac accgaatggt cggccgccac
     1981 caacggctcg caatcagctg ccgcgctgga ggtgtcgtcg ggtacgcccg tgatggcgat
     2041 cggcgggtgg agtggcgatc cggtccccac gctgcagacg ttcatcgacg acgtacacaa
     2101 cggcaagatc tcctactacg tggaggccgg ccgcggcggc gcggccggac aggcggaccg
     2161 cggcgaggtg atctacgggc gcagccgcgg cgctgcgcac acccgcgaga tcgccgaatg
     2221 ggtgatcaac cactttcagg gcaccgtcat cggcgaatcg acggtctacc gcctgacgta
     2281 gcatcgggcc cgtgtccagt acccagccgc ccgatgccgc gccggctgat ccgcccatcc
     2341 ccgtccccga cgtccccggc gccgacgcag gagcgcgcgg gctgccgcgg cgcgtggatc
     2401 tcacgctgcg ccaacgactg atcgtcgata cctcggcaat cgccgacctc ggcctgcgca
     2461 ccgcacttgc gtcggtggtc ggtgcggcga tggtgccgca gatcgcgttg gcagcactca
     2521 acggatccaa cgccaaggag cagcgcgacg cgctgcggtt ctacgccgag ctgggtgcgg
     2581 tcaaggacgc cggactgtcg ttccccaccc cgaccgaggt gccgcgaatc tcctcacgcc
     2641 cggcgaaccc catcgcggaa tggacggcca acggccgcgt gcacaacatc
//
LOCUS       DASYLX010001029         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2589998,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001029 DASYLX010000000
VERSION     DASYLX010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589998"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..737
                     /locus_tag="VGP27_24175"
     CDS             66..737
                     /locus_tag="VGP27_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEV7854430.1"
                     /translation="MWITLLAMAVAVSLEPFRIGMTILMMNRPRPLLQLLAFLAGGFA
                     MGIAVGVIVLFILRPALGSAHFTLPRVQIVVGVLLLINAAVVATGVGGKRSDGSAGPA
                     SRRFEPLAMRVRQLLNGCSLWTAGVAGLGIALPSVDYLAVLALIVASGAAASVQMGAL
                     LMFNVVAFGFVEIPLISYLVAPDRTRATLSALQDWLRTRRRSAVAILLTAVGCVLLGA
                     GLTGL"
     gene            complement(742..2490)
                     /locus_tag="VGP27_24180"
     CDS             complement(742..2490)
                     /locus_tag="VGP27_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854431.1"
                     /translation="MTPSPQSSILSMLHGRASLRPNDIAFTFTDYEKDWAGVPESVTW
                     SQLSRRTMNVAHELGLHASVGDRAVILAPQSLDYVAAFLGSMQAGLIAVPLPLPHRGS
                     SDERVGAVLADTSPSVVLTTSTAAEDVGDYIDQSRMDTAPKIVEIDSMNLDADGGPGI
                     PIADFPSTAYLQYSSGSTRLPTGVMISHRNLQVNFEQLMRSLFADANANSSTDVTIVS
                     WLPFYHDMGLVLGVCAPILGGFPAKLTSPVAFLEKPSRWMRALAENPRAFSGAPNFAF
                     DLAARKTSDSDLAGLDLGGVRGIINGAERVDSATLERFADRFAHFNFRDHMLRPSYGL
                     AEATVFVTSGTWSESSEAVRFDVDELSAGRAQRSLAGAGTDLVKYRVPQSPTLRIVDS
                     DTDRECPDGVVGEIWLHGDNVAAGYWSRPPEEQQCFGATLVDPSPGAADGPWLRTGDL
                     GFISDGELFIVGRIKDLLIIRGRNHYPEDIEATVQEITGGRVAAIAVPVNSTEKLVTV
                     IELKKRADSTGEAMHWLTGVKSDVTSAISNAHGVNVEDLVLVPPGSIPTTTSGKIRRA
                     ACVEQYRQDQFTRLDA"
BASE COUNT          439 a          827 c          820 g          492 t
ORIGIN      
        1 cggtccattt tcacacgttg ccgcgcccgg cggccacgtg cgccgggcgg taacctgacg
       61 ctgtcatgtg gatcacgctg ctggcaatgg ccgttgccgt cagcctggag ccgtttcgga
      121 tcggcatgac gatattgatg atgaaccgcc ctcggccgtt gctccagttg ctcgcgttcc
      181 tcgcaggcgg attcgcgatg ggcattgcgg tgggcgtgat cgtgctgttc attctgcggc
      241 ccgccctggg gtcggcccat ttcaccctgc cacgggtgca gatcgtcgtc ggcgtgctgc
      301 tgctgatcaa cgccgcggtg gtggccacgg gcgtgggggg caagcgcagc gacggctctg
      361 cgggtccggc gtcacggcga ttcgaaccgc tcgcgatgcg agtccgacaa ctcctcaacg
      421 ggtgttcact gtggacggcg ggtgtcgcgg gtcttggtat cgcgctgccg tccgtcgact
      481 accttgccgt gctggcactc atcgtcgcct cgggtgccgc ggcatccgtc cagatgggcg
      541 ccctgctcat gttcaatgtg gtggccttcg ggttcgtgga gattccgctg atctcctacc
      601 tggtggcccc ggaccgcacc cgcgcaacgc tgtcggcgtt gcaggactgg ctgcgaacgc
      661 gccgccgcag tgcggtcgcc attctgttga ccgcggtcgg ctgcgtgctg ctcggcgcgg
      721 gactcaccgg tctgtgacgg ctcaggcgtc caaccgggtg aactggtcct ggcggtactg
      781 ctcgacgcag gcggcccgcc ggatcttgcc gctcgtcgtg gtgggaatcg atcccggcgg
      841 taccagaacg agatcctcga cattcacacc gtgcgcattg gaaatcgcgg aggtgacgtc
      901 gctcttgacc ccggtgagcc agtgcatcgc ctctcccgtg gaatctgccc gcttcttcag
      961 ctcgatgacg gtgaccagct tctcggtgct gttcaccggg accgctatcg cggcaacccg
     1021 accgcctgtg atctcctgga cggtggcctc gatgtcctct ggatagtgat tacgtcctcg
     1081 gatgatcagc agatccttga tgcggccgac aatgaacagc tcgccgtcgg agatgaagcc
     1141 caggtcgccg gtcctcagcc agggtccgtc cgccgcgccc ggtgacgggt cgacgagcgt
     1201 cgcgccgaag cactgctgct cttccggtgg cctgctccag tagccggccg cgacattgtc
     1261 gccatgcaac cagatctcgc cgactacgcc gtccgggcac tctcggtctg tgtcggaatc
     1321 gacaatccgc aacgtgggcg attgcggcac cctgtacttg accaggtcgg tgcctgcccc
     1381 tgccaaagac cgctgagcgc ggcccgcgga caattcgtcg acatcgaaac ggacagcctc
     1441 cgacgattca ctccaggtgc cactcgtcac gaagaccgtt gcctccgcca ggccgtatga
     1501 cggacgaagc atgtggtccc gaaaattgaa gtgcgcaaac cgatctgcga agcgttccaa
     1561 cgtcgccgaa tcgacacgtt cggcgccgtt gatgatgccc cgcaccccac cgagatcgag
     1621 cccggcgagg tcgctgtcgc tggtcttgcg ggcggccagg tcgaaggcga agttgggcgc
     1681 ccccgaaaag gcacgcgggt tctcggccag cgcgcgcatc cacctggatg gcttctcaag
     1741 gaacgcaacc gggctcgtca gctttgcggg aaagccaccg aggatgggcg cgcagacccc
     1801 cagcaccaat cccatgtcgt ggtagaaggg cagccacgac acgatcgtga catcggttga
     1861 ggaattggcg ttcgcatccg cgaacaagct acgcatcagc tgctcgaagt tcacctggag
     1921 gttgcggtgc gagatcatca ccccggttgg cagtcgggtc gaacccgagc tgtactgcaa
     1981 atacgccgtg ctagggaaat cagctatcgg aatacccggt ccaccatcgg cgtccagatt
     2041 catcgaatcg atctcgacga tcttcggagc ggtgtccatc cgcgattgat caatgtagtc
     2101 gccgacgtct tccgcggccg tagacgttgt gagaacaacc gacggcgacg tatcggccag
     2161 gactgcacct acccgctcat cactcgagcc gcggtgcggc agcgggagcg gaaccgcgat
     2221 cagcccagcc tgcatggatc ccaggaatgc cgcaacgtag tcaaggcttt gcggagccag
     2281 gatcaccgct ctgtctccga ccgacgcatg aaggccgagc tcgtgtgcca cattcattgt
     2341 tcggcgggat aactgtgacc acgtgacact ctcgggaacg cccgcccagt ccttttcgta
     2401 gtcggtgaac gtgaacgcga tgtcattggg gcgcaggctg gcacgtccgt gcagcatcga
     2461 cagaatcgaa gactgaggcg aaggcgtcac atcacgtccg tagtaactag tcgaattggt
     2521 cgggctggaa gtcgatgtca gccgccgctg tggttcagca cgtactggaa gccggcca
//
LOCUS       DASYLX010001030         6190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3469615,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001030 DASYLX010000000
VERSION     DASYLX010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469615"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGP27_24185"
     CDS             complement(<1..330)
                     /locus_tag="VGP27_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV7854432.1"
                     /translation="MTTSSGTRQSRSVLQVGPLKPSLAQTLRDDYAAYVLPDEPAQRD
                     TFLSSHGTDITAVVTSGRSGVDAALMSALPNLGAVVNFGVGYDTTDVDAAAERGVAVS
                     NTPDVLTD"
     gene            638..2221
                     /locus_tag="VGP27_24190"
     CDS             638..2221
                     /locus_tag="VGP27_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7854433.1"
                     /translation="MSPAKRTPRRRITAAAAVALAGITTLIASGCTVANSRHGGYDPN
                     TLRVVLQQEPPTLEACESSLTSTGIVVRSNITEPLIERDPNSGDLQPLLATKWHQTSP
                     TKWEFDIRNGVTFSNGAPFTAQDAAFSIDRAVNSDLNCNVDGYVFGDDPLELSTPDDN
                     TLVVGTKTPDPILPLRISFVEIVPRGTSTKEKVREPIGTGPYAIEDWEYGQKLLLKRN
                     DAYWGKKPAFVHAEYQWRSEGSVRAAMITNDETDIATGLGPEDGAGDLGVPFQNNETT
                     ALRMQATEAPLNDMRVRQAINYAVNRDGIVEALFRGLGQPASQLIPSGVVGYNDELKL
                     WPHDQEKAKKLIAEAKADGVPVDRQIRLIGRTAQFPNISETIEVLQSEFTDIGLNVKI
                     EMMDTSNQLLYQLRPFPEGAGPYLLMIMHGNQAGDAAFTMDQYMLSDGPQAAYGTSEF
                     DKKIRAAEALTGQQRQDAFANLFAEEPREIMQMAYIAHMRGILGKSARVDYKPNSATG
                     DEMRLAEMTPAGADRTDQS"
     gene            2267..3196
                     /locus_tag="VGP27_24195"
     CDS             2267..3196
                     /locus_tag="VGP27_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854434.1"
                     /translation="MFPFVRRRMYTSALPLIIVLLGVFLIARLTGDPTNLYLPESATP
                     EQRAQFASQNGFDQPVWAQLVDYFKGVAHLDFGTSLRTGESASTMALRAFPATLQLAI
                     TTMLLAAVMAVVIGCWAAYRPNSLADRFSSLLSMTAASIPDFWFAITGVWLFAVLLGV
                     LPTSGVDSGILSWILPIATLMIRPLGVLTQVVRGAMVSALSAPYVRLARSKGASDFRV
                     VTHHALRNAAAPALTVAGDLAVGLINGAVVVEAIFGWPGIGKLMIDAILQRDFAVLQA
                     AVLLTAVSIFVLNIVIDACYALLDARVREPVMA"
     gene            3210..4217
                     /locus_tag="VGP27_24200"
     CDS             3210..4217
                     /locus_tag="VGP27_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854435.1"
                     /translation="MSTQLDLVPVKPTTAIVGEAGTDGGTTSVGSSRRSSQWLRLLVN
                     DRVATVAAVILGLVFLTAVFGPMLVGNLATHIDLDNSNQAPFTLAHGWANVLGTDPLG
                     RSMLARLIVACRTTLSVAIPAVVISAVVGSMIGMWAGYYRGWRETIAMRVADVIMSFP
                     SLLLAVVVLYVFSPSAANIVAVLAITRIPVYLRTARAESAELQSRLFVDAARTFGASA
                     RSIIWRHVLPVVLPTLLTVATLDFCYVMLAESSLSFLGIGIQPPDISWGLMVAQGRTY
                     LHTAWWLSFFPGVAIVITTVSATILAAWARIATDPAQRWRLTVPQKRLSKFTKTTKAL
                     S"
     gene            4214..5263
                     /locus_tag="VGP27_24205"
     CDS             4214..5263
                     /locus_tag="VGP27_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7854436.1"
                     /translation="MTAVIDHTAPVDRVDADPVLDVDGMTVDIRTITGTVRAVNEVSF
                     QARRGETLALLGESGCGKSMTATALVGLLEPVADIVGGTATLGDVEILGAGRKKRREI
                     AGTELAIVFQDALTALNPLYTVGTQLAEPFRIHHGMSAKVAKAKAVELMVRVGIPQPE
                     TRVNSYPHQFSGGMRQRLLIAMAVALNPSVLIADEPTTALDVTVQAQIMALLRDLRSE
                     YRMAVVLITHDLALVAEEADRVAVMYAGNIVETGPVADVFANPKHPYTRGLLDSVPVH
                     ARRGDELKSIGGSPPDLHAIPEGCVYQARCPLAREICAAKRPVLETVATGREAACHFP
                     EEVSPRTRGGEPTDV"
     gene            5256..>6190
                     /locus_tag="VGP27_24210"
     CDS             5256..>6190
                     /locus_tag="VGP27_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7854437.1"
                     /translation="MSESEKLLEVRDLRKSFSVPNAGKKRLCALDGITLDLARGETLG
                     LVGESGCGKSTLARTLMMLERPDSGTVRFDGIDPFSLRGKDLLKFRRRVQMVFQDPYA
                     SLNARMSAADIIAEPWHSHKGMYRDKRERDMRVRGLLDLVGLGAKAADKYPQEFSGGQ
                     RQRIGIARALALDPDVIICDEPVSALDLSVQAQVLNLLNDLQQQLQISYVFISHDLSV
                     VRHVADRVAVMYLGRIIESGPTEAVFERPDHPYTAALMSAAPTLDGVQRRERILLEGE
                     VPSPLNPPSGCRFRTRCWKATEVCATTAPPSALDL"
BASE COUNT         1053 a         2228 c         1896 g         1013 t
ORIGIN      
        1 gtcggtcagt acgtcgggcg tgttgctgac ggcgacccct cgttccgccg cagcgtcgac
       61 atccgtcgtg tcgtagccga ccccgaagtt caccaccgcg ccgaggttcg gcagcgccga
      121 catgagggcg gcgtcgacgc cggagcgccc ggacgtcacc actgcggtga tgtccgtgcc
      181 gtgggaggac aggaacgtgt cgcgctgggc cggttcgtcg ggcagcacat acgccgcgta
      241 gtcgtctcga agggtctgcg cgagcgacgg cttcagcggc ccgacctgta ggacggacct
      301 gctctgccgg gtgccggacg aagtagtcat gatgcgacaa cggtacggcg acctgcgatt
      361 acccgtccaa catccaatta gcatcgactt atgcccaacc agcatagcta gctaattcag
      421 acgacctgca aggtatcgcc ggtcgatcga tggcaataac gtgcctggtc agtggccatt
      481 tagcagatcc tgactggttc gaacgagagg cccattaccc gctgatatgg ccatttcgcc
      541 ggggttgacg gtccggaacg gacgtcctta ccttgtgtct gcgatcacag ctcttatcca
      601 catacgtgga cgtcacaacc gcagcccggg aggatccatg agccccgcaa aacgcacacc
      661 gcgccggcgc atcaccgcgg ccgccgccgt cgcccttgcg ggaatcacga ccctcatcgc
      721 gagcggctgc accgtcgcca actcgcgcca cggcggctac gacccgaaca ccctgcgcgt
      781 cgtgctgcag caagagcctc ccacgctgga ggcctgtgag agctccctga cctccaccgg
      841 catcgtcgta cgctccaaca tcaccgagcc cctcatcgag cgcgacccga actcgggcga
      901 cctgcagccg ctgctggcca ccaagtggca ccaaacctcg cccaccaaat gggagttcga
      961 catccgcaac ggcgtgacgt tctccaacgg cgccccgttc accgcgcagg atgccgcctt
     1021 ctccatcgac cgcgccgtga actccgacct caactgcaac gtcgacggct acgtgttcgg
     1081 cgacgacccg ctcgagctct ccacgcccga cgacaacacc ctcgtcgtgg ggaccaagac
     1141 tcccgatccg attctgccgc tgcgcatctc gttcgtcgag atcgtgcctc gcggcaccag
     1201 tacgaaggag aaggtgcgcg agccgatcgg caccgggccc tacgccatcg aggactggga
     1261 gtacgggcag aagctgctcc tgaagcgcaa cgacgcctac tggggcaaga agccggcctt
     1321 cgtgcacgcc gagtaccagt ggcgcagtga aggcagcgtg cgcgccgcga tgatcaccaa
     1381 cgacgagacc gacatcgcga ccggactcgg ccccgaggac ggcgcgggcg acctcggtgt
     1441 tccgttccag aacaacgaga ccaccgcgct gcggatgcag gccaccgaag ccccgttgaa
     1501 cgacatgcgc gttcgacagg ccatcaacta cgccgtcaac cgtgacggta tcgtcgaggc
     1561 gcttttccgc ggcctgggac agcctgcctc gcaattgatt ccgtccggcg tcgtcggtta
     1621 caacgacgag ctgaagctct ggccgcacga ccaggagaag gcgaaaaagc tcatcgccga
     1681 ggcgaaggcc gacggtgtac ccgtcgacag gcagatccgg ctgatcggcc gcaccgcgca
     1741 gttcccgaac atcagcgaga ccatcgaagt gctgcagagc gagttcaccg acatcggcct
     1801 gaacgtcaag atcgagatga tggacacgtc caatcagctg ctgtaccagc tgaggccgtt
     1861 cccggagggt gcgggcccct acctgctgat gatcatgcac ggcaaccagg ccggtgacgc
     1921 agcattcacc atggaccagt acatgcttag cgacggccct caagccgctt atggcacatc
     1981 ggaattcgac aagaagatcc gggccgccga agcactcacc ggccagcagc gtcaggatgc
     2041 cttcgccaac ctcttcgccg aggagcccag ggagatcatg caaatggcct acatcgcgca
     2101 catgcgcggc atcctcggca agtccgcgcg tgtcgactac aagcccaact ccgccaccgg
     2161 cgacgagatg cgcctcgccg agatgacgcc ggccggcgcc gaccgaaccg accagtccta
     2221 gaagctcagg cactttcccg ccaagcccac ccggaaggcc agccctatgt ttcccttcgt
     2281 ccggcgccgg atgtacacga gcgcactgcc gttgatcatc gtcctgctcg gcgtctttct
     2341 gatcgcccgg ctcaccggcg atcccaccaa tctgtacctg cccgagtcgg cgacgcctga
     2401 gcagcgcgcg cagttcgcgt cgcagaacgg tttcgaccag ccggtgtggg cgcagctcgt
     2461 cgactacttc aagggcgtcg cacacctgga cttcggcacc tcgctgcgca ccggcgagtc
     2521 ggcctcgacg atggcgctga gggcgttccc cgccaccctg caattggcca tcaccacaat
     2581 gcttctggcg gccgtcatgg ccgtcgtcat cggctgctgg gccgcctatc ggcccaactc
     2641 gctggccgac cgattctcca gtcttctttc catgaccgcc gcgagcatcc ccgacttctg
     2701 gttcgccatc accggcgtct ggctgttcgc ggtcctgctg ggcgtgctgc ccacctccgg
     2761 tgtggacagc ggcatcctct cctggatcct gccgatcgcc accctgatga tccgtccgct
     2821 cggcgtgctg acccaagtgg tccgcggcgc aatggtttcc gcgctgtcgg cgccctacgt
     2881 gcggctggcc cgcagcaagg gcgccagcga cttccgcgtc gtcacccatc acgccttgcg
     2941 caacgccgcc gcacccgcgc tgaccgtggc aggcgacctg gccgtgggac tcatcaacgg
     3001 cgcggtcgtc gtcgaggcca tcttcggctg gcccggcatc ggcaagctga tgatcgacgc
     3061 gattttgcaa cgcgacttcg cggtgctgca ggcggcggtg ctgctgaccg cggtgagcat
     3121 cttcgtcctc aacatcgtca tcgacgcctg ctacgccctg ctcgacgccc gcgtccgcga
     3181 accggttatg gcctgaggag acacgacaca tgagcactca actcgatctg gttcccgtga
     3241 agccgacgac ggcgatcgtc ggcgaggcgg gaacggacgg cggcaccacg tccgtcggct
     3301 cctcgcgccg ctcgtcgcag tggttgcggc tgctggtcaa tgaccgggtc gccaccgtcg
     3361 cggcggtcat cctcggcctg gtcttcctca ccgccgtctt cggcccgatg ctcgtaggca
     3421 acctcgccac ccacatcgac ctggacaact ccaaccaggc gccgttcacg ctcgcacatg
     3481 gctgggccaa cgtgctgggc accgaccccc tcggccgcag catgctcgcc cgcctgatcg
     3541 tcgcctgccg cacgacgctg tccgtcgcga tccccgcagt ggtcatctcc gccgtcgtcg
     3601 gctcgatgat cggtatgtgg gccggctact accgcggttg gcgcgaaacg atcgcgatgc
     3661 gggtcgccga cgtcatcatg agcttcccgt cgctgctgct ggccgtcgtc gtgctctacg
     3721 tgttctcccc cagcgccgcg aacatcgttg cggtgctggc catcacccgc atcccggtat
     3781 acctgcgcac cgcccgcgcc gagtccgccg agctgcagag ccggctgttc gtcgacgccg
     3841 cgcgcacgtt cggcgccagc gcgcggtcga tcatctggcg ccacgtgctg cccgtcgtgc
     3901 tgcccaccct gctgaccgtc gcgacactgg acttctgcta cgtcatgctc gccgagtcat
     3961 cgctgagctt cctgggcatc ggcatccagc cgcccgacat cagctggggc ctgatggtcg
     4021 cgcagggccg cacctacctg cacaccgcct ggtggctgtc gttcttcccc ggtgtggcga
     4081 tcgtgatcac cacggtgtcg gccaccatcc tcgccgcgtg ggcccgcatc gccaccgatc
     4141 ccgcacagcg gtggcggctg accgtcccac agaaacgcct gtccaaattc accaagacca
     4201 cgaaggcact ctcatgaccg ctgtcatcga ccacacggcg cccgtcgatc gggtcgacgc
     4261 cgaccccgtg ctcgacgtcg acggcatgac agtcgacatc cgcaccatca ccggcacggt
     4321 ccgcgctgtc aacgaggtga gcttccaggc tcgacggggc gaaacactgg cgctgctcgg
     4381 cgaatccggc tgtggcaagt cgatgacggc caccgcgctc gtcgggctgc tcgagcccgt
     4441 cgccgacatc gtcggcggca ccgccaccct cggcgacgtc gagatcctgg gcgccggccg
     4501 caagaagcgg cgcgagatcg cgggcaccga actggcgatc gtcttccagg atgcgctgac
     4561 cgcgctcaac ccgctctaca ccgtgggaac tcaactggcc gaaccgttcc ggatccacca
     4621 cggcatgagc gccaaggtgg ccaaggccaa ggccgtcgag ctgatggtcc gcgtcggcat
     4681 cccgcaaccc gagacccggg tgaactccta cccgcaccag ttctccggcg gcatgcggca
     4741 gcgtctgctc atcgcgatgg ctgtcgcctt gaatcccagc gtgctgatcg ccgacgagcc
     4801 gaccaccgcg ctcgacgtca ccgtgcaggc gcagatcatg gccctgctgc gggatctgcg
     4861 cagcgagtac cggatggccg tcgtgctgat cacccacgac ttggcgctgg tcgccgagga
     4921 ggccgaccgc gtcgccgtga tgtacgccgg caatatcgtc gagaccgggc cggtggcaga
     4981 tgttttcgcc aatccgaagc acccgtacac caggggtttg ctggactcgg ttccggtcca
     5041 cgcgcgccgc ggcgacgagc tcaagtcgat cggcggctcg ccgcccgacc tgcatgcgat
     5101 ccccgagggc tgcgtatatc aggcgcgttg cccgttggcc cgcgagatct gcgccgccaa
     5161 acgccccgtc ctggaaaccg tggcgaccgg ccgcgaggcc gcgtgccact tccccgaaga
     5221 ggtttcgccc cgcacccgag gaggagaacc aacagatgtc tgagtctgag aagctgctcg
     5281 aggtccgcga ccttcgcaaa tcgttcagcg tgccgaacgc gggcaagaaa aggctgtgcg
     5341 cactcgacgg catcaccctc gacctggccc gcggcgaaac cctcggcctc gtcggtgagt
     5401 ccggctgcgg aaagtccacg ctggcaagga cactgatgat gctggagcgg cccgattcgg
     5461 gcaccgtgcg cttcgacggc atcgacccgt tcagcctgcg cggcaaggat ctgctgaagt
     5521 tccgccgccg cgtgcagatg gtattccagg acccgtacgc ctcgctgaac gcgcggatgt
     5581 cggcggccga catcatcgcc gagccgtggc acagccataa gggcatgtac agggacaagc
     5641 gcgaacgcga catgcgggtg cgcgggctgc tcgacctcgt cggcctcggc gcgaaggcgg
     5701 ccgacaagta cccgcaggaa ttctcaggcg gtcagcggca acgcatcggc atcgcccgcg
     5761 ccctggcgct cgaccccgac gtgatcatct gcgacgagcc ggtctccgcg ctcgacctct
     5821 ccgtgcaggc acaggtgctc aacctgctca acgaccttca gcagcagctg cagatctcct
     5881 atgtgttcat ctcgcacgac ctgtcggtgg tccgacatgt cgccgaccgg gtcgcggtga
     5941 tgtatctcgg ccggatcatc gaatcggggc cgactgaagc ggttttcgag cgacccgacc
     6001 atccctacac cgcggcgctg atgtccgctg cgccgacgct ggacggcgta cagcgccgcg
     6061 agcgcatcct gctcgagggt gaggtgcctt cgccgctaaa cccgccgtcg ggttgccggt
     6121 tccggacccg ctgctggaag gccaccgagg tctgcgcgac caccgcgccg ccgagcgcgc
     6181 tcgatctgga
//
LOCUS       DASYLX010001031         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1493433,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001031 DASYLX010000000
VERSION     DASYLX010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493433"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VGP27_24215"
     CDS             complement(<1..998)
                     /locus_tag="VGP27_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV7854438.1"
                     /translation="MSSKDSFGAHDTLKVGDKSYEIYRLDAVPGTEKLPYSLKVLAEN
                     LLRSEDGSNITKEHIEAIANWDPDADPSVEIQFTPARVIMQDFTGVPCIVDLATMREA
                     VGDLGGDRDKVNPLAPADLVIDHSVIADLFGRADAFERNVEIEYQRNGERYQFLRWGQ
                     GAFNDFKVVPPGTGIVHQVNIEYLARVVWERDGVAYPDTCVGTDAHTTMENGLGVLGW
                     GVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIQAGVTATDVVLTVTEKLRKHGVVGKF
                     VEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETISYLKFTGRKPEQLRSHG"
     gene            1159..1695
                     /locus_tag="VGP27_24220"
     CDS             1159..1695
                     /locus_tag="VGP27_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6676 family protein"
                     /protein_id="HEV7854439.1"
                     /translation="MTGPHVIPFLPSYIPPDVDMNQVLADVGDDGVSVPPADAKAVPE
                     LRQVIADAHQHGIDLKIVVIASNPPIDTPLRDIATEVGQAYPASTVLAMSPTWAGTYS
                     KEFDRATLEAGQDIAKNGDPVQSSKNFVSELTTPHFPWTTFSVVLIVLVLAAVVAARS
                     LQVRSKADAAQIEPKASE"
     gene            1882..3270
                     /gene="ripA"
                     /locus_tag="VGP27_24225"
     CDS             1882..3270
                     /gene="ripA"
                     /locus_tag="VGP27_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan endopeptidase
                     RipA"
                     /protein_id="HEV7854440.1"
                     /translation="MRRTPRALTSLGILVVTALYIPGGLAAAEPSSPDSVGSLVAAVA
                     NVNQKLQELGAAVQAQQEGVNKAIVDVQTARDNAAAAQQDVDANKRAVDDSTAAITSA
                     QKRFDTFAASTYVSGPSSSYLTAADPADIIDTASAGQTLATSAQQIVADLQRARTEQV
                     NKESAARLAKEKADQATVNAQSSQDAAVSALKSAQQTFADQQGRLTELTAERNAAQAK
                     LAEARAWSAPASGRSKPVAPLANPSANWDRAPGTPAPAAGNWDQSLWDPTLPAIPSAF
                     VSGDPIAIINSVLGMSSTSAQYTADMGRNFLRKMGILPTPTGYTNGAIPRVYGRQASE
                     YVIKRAGSQLGVPYSWGGGNAAGASTGIDSGSNTVGFDCSGLILYAFAGVGIKLPHYS
                     GSQYDLGRKIPSSQMRRGDVIFYGPGGSQHVTLYLGNNQMLEAPYTGSHVKVSPVRTS
                     GMTPYVIRYIEY"
     gene            3384..>3712
                     /locus_tag="VGP27_24230"
     CDS             3384..>3712
                     /locus_tag="VGP27_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C40"
                     /protein_id="HEV7854441.1"
                     /translation="MPAAAAPEDGQWDPTLPKIVSSGAPGDPVAIANASFQVSQLALQ
                     TTSNLGQQFLSSIGLGGSTSSQGAVMPGGRVRGPQAIEYAIRRGGSQMGVPYSWGGGT
                     LTGPGPGV"
BASE COUNT          652 a         1222 c         1179 g          659 t
ORIGIN      
        1 ccgtggctgc gcagctgctc gggcttgcgg ccggtgaact tcaggtagga gatggtctct
       61 tcgtcgatcg ggaaaattgc tgcggtggaa ccgaattcgg ggctcatgtt gcccagggtg
      121 gcgcggttgg ccagcggcac ctcggccacg ccttcgccgt agaactcgac gaacttgccg
      181 acgacgccgt gcttgcgcag cttctcggtc accgtcaaca cgacgtcggt ggcggtgacg
      241 ccggcctgga tctcgccggt cagcttgaag ccgacgaccc gggggatgag catcgacacc
      301 ggctggccca gcatcgcggc ctcggcctcg atgccgccga cgccccagcc gagcactccg
      361 aggccgttct ccatcgtggt gtgtgcatcg gtgccgacgc aggtgtccgg ataggcgaca
      421 ccgtcgcgct cccacaccac gcgtgccagg tattcgatgt tgacctggtg cacgatcccg
      481 gtgccgggcg gtaccacctt gaagtcgttg aatgcgccct ggccccagcg caggaactga
      541 tagcgctcgc cgttgcgctg gtattcgatc tccacgttgc gctcgaacgc gtccgcgcgg
      601 ccgaagagat cggcaatcac cgagtggtcg atcaccagat cggcgggtgc cagcggattg
      661 accttgtcgc ggtcgcctcc gaggtcgccg acggcctccc gcatggtggc caggtcgacg
      721 atgcagggca cgccggtgaa gtcctgcatg atgacgcggg cgggggtgaa ctggatctcc
      781 acgctcggat cggcgtccgg atcccagttc gcgatcgcct cgatgtgctc cttggtgatg
      841 ttggagccgt cctcgctgcg aagcagattc tcggcgagca ctttgaggct gtagggaagt
      901 ttctcggttc cgggtaccgc gtccaagcgg tagatctcat agctcttgtc cccgaccttc
      961 agggtgtcgt gggctccaaa cgaatccttg ctgctcacat caactcccgg ctaagtaatc
     1021 gccgcgacgg gctgtgtcgg cggctcttcc aatctaacag tacgcttgtc ctgtatggca
     1081 agcccggacg gctccagtct tgtcttgacg cgtgcgtgct gccactttcc gcggggcgta
     1141 tcgcgtacgg tttctcctgt gaccggcccg cacgtgatcc cgttcctccc gtcgtatatc
     1201 ccgccggacg tcgatatgaa ccaagtgctc gcagatgtcg gcgatgacgg cgtgagcgtg
     1261 ccccccgccg atgctaaagc cgtgcccgaa ttgcgccagg tgatcgccga cgcacaccag
     1321 cacggcatcg atctcaaaat cgtggtcatc gcctccaatc cgccgatcga caccccgctg
     1381 cgcgatatcg cgaccgaggt ggggcaggcg tacccggcct cgaccgtgct ggcgatgagc
     1441 ccgacgtggg caggcaccta cagcaaggag ttcgatcggg cgacgctcga ggccggccaa
     1501 gatatcgcga aaaacggcga ccccgttcaa tcgtcgaaga atttcgtgag cgagttgacg
     1561 acgcctcatt ttccgtggac aacattctcc gtagtgctca ttgtcctggt tttggccgcc
     1621 gtcgtcgcgg cccggtcgct gcaggtccgg agcaaggccg acgcggccca aatcgagccg
     1681 aaagcctccg aataagcccg gcataacgct gggcataagc agacggcgaa ttcgaatatc
     1741 accattcggt cacactccgt ttaattacag atatgtaatt tgtgactaga gtttcgttag
     1801 cgtcagttgt gacgtacggt gcagaagtac ctgctggtgt agttgttgtc gttgtgactt
     1861 ctgcgaggag acctgagtcg gatgagacgc acccctcgcg cgctcacatc cctcggcatc
     1921 ctcgtggtaa ccgccctcta catacccggc ggattagcgg ccgccgaacc cagtagtccg
     1981 gattccgtcg gatcgctggt cgcagctgtg gcgaacgtca accagaaact gcaggaactc
     2041 ggcgcagccg tgcaggcgca gcaggaaggc gtcaacaagg ccatcgtcga tgtccagacc
     2101 gcacgcgaca acgcggcggc ggctcaacag gatgtggatg ccaacaagcg cgcggtcgac
     2161 gactcaaccg ccgcgatcac gtccgcgcag aagcgattcg acaccttcgc cgcgtcaacc
     2221 tatgtcagcg ggccgtcgag ttcgtacctg acggcggccg accccgctga catcatcgac
     2281 accgcttcgg cgggccagac gctggccacc agcgcacagc agatcgtggc ggacttacag
     2341 cgggcgcgca ccgagcaggt caacaaggag tccgcggcac ggctggccaa ggagaaggcc
     2401 gaccaggcca cggtgaacgc gcagtccagc caggacgcgg cggtgtctgc gttgaagtcc
     2461 gcccagcaga ccttcgccga ccagcagggc cggctgacgg agttgaccgc cgagcgtaac
     2521 gccgcccagg ccaagctggc cgaagcccgc gcttggtcgg cgccggcgtc aggccggtcg
     2581 aaacccgtgg cgccgctcgc gaacccgtcg gcgaattggg accgcgcgcc gggtactcct
     2641 gcgcccgctg cggggaactg ggaccagagc ctgtgggatc cgacgctgcc cgcgatcccg
     2701 agcgcgttcg tcagcggcga cccgatcgca atcatcaact cggtgctcgg catgtcgtcc
     2761 acctcggcgc agtacaccgc cgacatgggc cgcaactttc tgcgcaagat gggcattctg
     2821 ccgacaccga ccggctacac caacggcgcg attccccgcg tgtacggaag gcaggcctcc
     2881 gagtacgtca tcaagcgtgc ggggtcccag ctgggcgtgc cgtactcatg gggcggcggc
     2941 aatgccgccg gtgcgagcac cggcatcgat tccggctcca acacagtcgg attcgactgc
     3001 tcgggcctga tcctgtacgc cttcgcaggc gtcggtatca agttgccgca ctactcgggt
     3061 tcgcaatacg acctgggccg caagatcccg tcatcgcaga tgcggcgcgg cgacgtcatc
     3121 ttctacggac ccggcggcag ccagcacgtc acgctctacc tcggcaacaa ccagatgctg
     3181 gaggccccct acaccggctc tcacgtgaag gtttcaccgg tgcgaacaag cggcatgacc
     3241 ccgtacgtga tccggtacat cgaatactga tgctgctact gatgcgagtg attcgtttga
     3301 acttcatgcg ccacaacggc tcccgttgcg cgaggatcgt cgtaccggtt ttggcggcgc
     3361 tggctctggc actcgggttg gccatgccgg ccgcggccgc acccgaggac gggcaatggg
     3421 atcccacgct tccgaagatc gtcagttccg gcgcccccgg cgatccggtg gcgatcgcga
     3481 acgcctcgtt tcaggtgagc cagctcgccc tccagacaac ttcgaacctc ggacagcaat
     3541 tcctgtccag catcgggctc ggcggcagca ccagcagcca gggtgccgtc atgcccggag
     3601 gccgggttcg cgggccgcaa gcgatcgagt atgcgattcg acgcggcggt tcgcagatgg
     3661 gcgtgccgta ttcatgggga ggcggcacgc tcaccggccc cgggcccggc gt
//
LOCUS       DASYLX010001032         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2260131,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001032 DASYLX010000000
VERSION     DASYLX010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260131"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VGP27_24235"
     CDS             complement(<1..760)
                     /locus_tag="VGP27_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854442.1"
                     /translation="MRSLSYDELYIGGRWRPPATNRRITVISPHTEEPIGETPEASPQ
                     DVDRAVLAARKAFDEGPWPRLPVSERMEKIEKLIGIYTAHAEEMADLITAEMGSPRSF
                     SRLGQANGALSQMYLTLATARDFGWIERRQGLFGQVQVRRAPVGVVGAIVPWNVPQVL
                     IMPKLIPALIAGCTVLVKPAPETPLDAMWLAEMLEELDLPEGVVSILPGGRETGDALV
                     RHPGVDKIAFTGSSATGRHIAAACGEQLKRVSLEL"
     gene            832..2037
                     /locus_tag="VGP27_24240"
     CDS             832..2037
                     /locus_tag="VGP27_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEV7854443.1"
                     /translation="MGDDWRRYPFDLVPGHPHFVFPAAEANHPTCESDTWFLAGELTG
                     ESNRKFAFLTIFNKNRPGLAVVADFYTLALFDLDNGTYGTYTDYDMPSTNKKADVAPK
                     LTVADDHLDMTYDSCAGTALWRTCRDGAGDLLPYTYDVEFRGLDQHGSEMRLDLHVTP
                     SRAPVPLGAATYDGVIECFGQANTYSYFQTGMAMTGTLSWGSVQEKVSGTTGHVDRQW
                     FPLVANDGGGTGDIRSRAHEWRTINLDNSVDMSIWRQFDRRNHNALQPFSGATTSSPD
                     ADPEYADDIEVTVTSYVRWPETIRTLVPPPAAARYMPDRHRLTSRKLDLDLTGEPLVP
                     APAHGLPLEYMEGPYRYQGTFRGEPVSGFAFFERSLALYRDWELADVLEAAEPGERDE
                     VARLIEEQR"
     gene            complement(2018..>2585)
                     /locus_tag="VGP27_24245"
     CDS             complement(2018..>2585)
                     /locus_tag="VGP27_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7854444.1"
                     /translation="EKVLDTAINWKLAIDTFAENYHFATVHKTTFATIARSNCTVFDS
                     FGPHHRLVFPLNGILDLEEIPEEQWDPLQAMVVIYALFPNIVLSATIANGELFRVYPG
                     ALPGRSITVHQNSTPLDISDESTAAGAQAVFDYAHGTVRDEDYALVEKLQANLASGVR
                     EHLVFGRNEPGLQHRHEIWADAVSAALR"
BASE COUNT          463 a          850 c          824 g          448 t
ORIGIN      
        1 cgagttcgag gctgacccgt ttgagttgct cgccgcacgc ggcggcgatg tgtctgccgg
       61 tcgccgagga gccggtgaac gcgatcttgt ccacaccggg gtggcggaca agcgcatcgc
      121 cggtttcccg gccgccgggc aggatcgaca ccacaccctc cggcaggtcg agttcctcca
      181 gcatctcggc cagccacatc gcatccagcg gagtctccgg cgccggcttg acgagaacgg
      241 tgcaacccgc gatcagcgcg ggaatcagct tcggcatgat cagcacctgg ggcacattcc
      301 acggcacgat cgcgccgacg acgccgaccg gggcgcgccg cacctgaacc tggccgaaca
      361 ggccctgcct gcgctcgatc catccgaaat ccctggcggt ggccagcgtc aaatacatct
      421 gtgacagcgc gccgttagcc tggcccagcc tgctgaagga gcgcggcgaa cccatctcgg
      481 cggtgatgag atcggccatc tcctcggcgt gcgcggtata gatgcctatg agcttctcga
      541 tcttctccat ccgctcggag accggaagcc gcggccatgg tccttcgtca aaagccttgc
      601 gcgcggccag caccgcccta tcgacgtcct gcggcgaagc ctcaggggtt tcgccgatgg
      661 gttcctcggt gtgtggggag atcaccgtga tccgccgatt ggtggcgggc ggtcgccagc
      721 gcccgccgat gtagagctcg tcgtaggaaa gggatctcac gcgcgtcacc cgcccgtcgc
      781 tctaatgttg ctattactgc taacatagca aaaaaggagt ctggagtgcc cgtgggtgat
      841 gactggcgtc gatatccgtt cgatctggtt cccggtcatc cgcatttcgt ctttcccgcc
      901 gcagaggcga accaccccac ctgtgaatct gacacctggt ttctcgcagg cgaactgacc
      961 ggcgaatcca atcggaagtt cgctttcctg acgatcttca acaagaaccg gcccgggctc
     1021 gccgtggtcg ccgacttcta cacgctcgcg ctgttcgacc tcgacaacgg cacctatggc
     1081 acctacaccg actacgacat gccgtcgacg aacaagaagg ccgatgtcgc acccaagctg
     1141 acggtcgccg acgaccacct ggacatgaca tacgacagtt gtgcagggac ggcgctctgg
     1201 cgtacctgcc gggacggggc cggcgacctg ttgccctaca catacgacgt cgaattccgc
     1261 gggctcgacc agcacggcag cgagatgcgg ttggacctgc acgtgacgcc gtcgcgcgca
     1321 cccgtgccgc tgggcgccgc aacctacgac ggggtcatcg aatgcttcgg ccaagccaac
     1381 acgtactcct acttccagac cggtatggcg atgaccggaa cgctgtcgtg gggttcggtg
     1441 caggagaagg tcagcggaac caccgggcac gtcgaccggc agtggtttcc gttggtggcc
     1501 aacgacggtg gggggaccgg cgacatccgc tcccgcgccc acgaatggcg gacgatcaac
     1561 ctggacaaca gcgtcgacat gagcatctgg cgccagttcg accgaaggaa tcacaatgcg
     1621 ctgcagccct tttcgggcgc cacgacgagc tcgccggacg cggaccccga gtatgcggac
     1681 gacatcgagg tcaccgtgac cagctacgta cgctggcccg agacgatccg cacgctggtt
     1741 ccgccgcctg ccgcggcacg gtacatgcct gatcgccatc gcctgacgtc gcgaaagctc
     1801 gacctggacc tgacggggga accgctggtg cctgcccccg cacacggcct gccgctggag
     1861 tacatggagg gcccgtatcg gtatcagggc acatttcgcg gcgagccggt cagcggcttc
     1921 gcattcttcg aacggtcgct ggcgctgtac cgggactggg aactggcgga cgtgctcgag
     1981 gccgccgagc cgggggagcg agacgaggtc gctcggctca tcgaagagca gcgctgacag
     2041 cgtccgccca tatctcgtgc cgatgctgca gccccggctc gttgcggccg aacaccagat
     2101 gttccctcac tccggacgcg agattggcct gtagcttctc gaccaacgcg taatcctcgt
     2161 cgcggacggt gccgtgcgcg tagtcgaaga ccgcctgcgc gcccgcggcg gtcgactcgt
     2221 ccgaaatgtc cagcggcgtc gagttctggt gcacggtgat cgagcggccc ggcagggctc
     2281 ccggataaac ccggaacagt tcgccgttgg cgatggtcgc cgacagcacg atgttgggga
     2341 acagtgcgta gatgacgacc attgcctgca gcggatccca ctgctcttcg ggaatctctt
     2401 ccaggtcgag gatgccgttc agcgggaaca ccagtcggtg atggggaccg aacgaatcga
     2461 aaacagtgca gttgctgcgc gcgatggtcg cgaacgtcgt cttgtgcacg gtcgcgaagt
     2521 ggtagttctc cgcgaaggtg tcgatcgcga gcttccagtt gatcgccgtg tccagcacct
     2581 tctcg
//
LOCUS       DASYLX010001033         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_616156,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001033 DASYLX010000000
VERSION     DASYLX010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616156"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGP27_24250"
     CDS             <1..454
                     /locus_tag="VGP27_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV7854445.1"
                     /translation="TVSGSVPPTVNLAERTEDGFGQGKVLVSPFGMAMAAATVAAGKT
                     PVPQLIEGRQTTVTGDHSEITPKMLDGLRPMMQLVVTNGTAKDINGMGDVRGKTGEAE
                     FEGGSHSWFAGYRGDMAFAALIVGGGSSEYAVRMLKWMLESLPPDYLA"
     gene            complement(736..1917)
                     /locus_tag="VGP27_24255"
     CDS             complement(736..1917)
                     /locus_tag="VGP27_24255"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV7854446.1"
                     /translation="MSTADRFQPAALIDQACQWAGCDDFGAEFDENQTWRDGLGRLCE
                     GFVEEARLNDLGVEIAASDIVRALKNRLQIVRWRKAHPAIAGERIERPIFIVGQPRTG
                     TTILFDLLAQDPELRPPLTWEVDAPLPVPQPDTYDTDPRIAETQTSLEMSEQIIPGFM
                     SWHPMGAQVGQECVRITASQFCSMIFSVQYRLPGYYKWLLYEADHRQAYRFHRIFLQH
                     LQSGVKGQWLLKSPAHLWTLDTLVETYPGAVIVQTHRDPLKVISSISALTNHLRRMAS
                     DETTVADCAVQSCEEIVVGLQRGMTLRESLPPGQVIDVQFTDFMRDPFATIRSLYGRL
                     DRQLTPIAEQRMREHLAANPGDGGGGRYSWADTGLDAEQLREQVAGYQQCYGVATEPL
                     A"
     gene            complement(1914..2948)
                     /locus_tag="VGP27_24260"
     CDS             complement(1914..2948)
                     /locus_tag="VGP27_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEV7854447.1"
                     /translation="MIGDITNIVTADAENERELLEGLGVISRVTALCSELTVEADPEL
                     PWFFDMCSPTRLVGGPNPDGRYLLAMIRGDRGYRVTGTRGTTAYLGVQVLAGTGMTPR
                     RMAAYVSDTDMKLDGGRLALVFSVTEPSPAELDGAQWVPIPEDATSIVVREYIADAHS
                     EHPATLHIDPLTTPQPPQKVSDDEAAEQFSAMAWTIMKLTTLHRTIKPELMRQPNTLV
                     TSEAADLGEADTTPDNLYMLGSFALGDDESLVLEFEPPDTRYWNITLENIWHECLEPR
                     RRHSSVTNKGVTPDADGVVRVAVGAKDSGHGHWLDTGGRHRGFVVFRWLDNPHPPKVS
                     VDVRIGEATR"
     gene            complement(3051..>6067)
                     /locus_tag="VGP27_24265"
     CDS             complement(3051..>6067)
                     /locus_tag="VGP27_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV7854448.1"
                     /translation="LAPIRLRIGVHTGQVQLRDEGNYIGPTINRTARLRDLAHGGQTV
                     LSGATEQLVADWLPDGAWLTDLGSHELRDLPRPERVTQLCHPDLSNDFPPLRVTRNAE
                     KHSLPGQLTSFVGRAPQIAEIRALLSDNRIVTLTGAGGAGKTRLAIQVAADIAPQYRD
                     GVSYVDLSPVTYEAVVPVAVARALGLPDQPGRSMTESLTKFVGDRQTLMVLDNCEHLL
                     DAAASLAAELLSACPALQILATSRESTGVPGEVTFQVPSLSLVDEALELFVDRARRVR
                     PEFTIAADNAPAVTEICKRLDGMPLAIELAAARVRALSLEEIVGGLHDRFRLLTGGAR
                     TAVRRQQTLRASVDWSHALLTETERVLFRRLAVFLGGFDLDAAQTVAGDAEVQRYQVL
                     DQLALLVDKSLVTAENTKGHMRYRLLETVRQYAQEKLGESGEAEAVRSRHRDHYNSMA
                     ALLDSPARSDYQLRVEQADDDMDNLRAAFGWSLESGDIASALELACSLQPLWLSRGRI
                     QEGLAWFDAALGDEKASRSDLAHPMRARVLADKVVLEAWVNTYSLGDAEQALAIAREH
                     DDPALMARALTACCSAAVYDAEAAPRYFGEALGLTRELGDQWRLSQLLGQQAHAALVA
                     GDVRALHAAAAEGRDVADAIGDRFGSRQCRWRLAGAQVFESDIADAIIEFRAVIAESE
                     TDHDTISRVAALLMMPHALIAAGDTEAARVAAEAGVEAGAELGDVFRGGSYIALMVTH
                     LAAGDVALAVDAADAAWPLIGQLQGTASINSVFIAALELARGDLDGARRWADTTIAAV
                     KGWTLGAALATRARIAIAEQKPEEAERDAHDALATWVDVGAYLGVPDILDVLAGVAGD
                     GARHREAARLFGAAAGIRQRTGEVRWRIYQAEYDASVAALRNAMEDADFDAAWAEGAA
                     LSRDEAIAYAQRGRGERRRPSSGWASLTPAELDVVRLVSEGLGNKDVATRLFISPRTV
                     QSHLTRVYTKLGLSSRVQLAQEAARRSTSSNS"
BASE COUNT         1011 a         1991 c         1996 g         1069 t
ORIGIN      
        1 aacggtttcc ggctcggtgc cgccgacggt gaacctggcc gaacgcaccg aggacggctt
       61 cggtcagggc aaggtgctgg tcagcccgtt cggcatggcc atggccgcgg cgacggtggc
      121 tgcaggcaaa acgcctgtgc cgcaattgat cgagggcagg cagacgaccg ttaccggcga
      181 ccacagcgag atcacgccca agatgctcga cgggctgcgg ccgatgatgc agctggtggt
      241 caccaacggc accgccaagg acatcaacgg tatgggcgat gtgcgcggaa agaccggcga
      301 ggccgaattc gaaggcggat cacattcctg gttcgccggc taccgcggcg acatggcgtt
      361 tgcagcgctg atcgtcgggg gcggcagctc ggagtatgcg gtgcggatgc tgaagtggat
      421 gctcgaaagc ctgccgcccg actacttggc ctgacgcccc ttgcggaatg tcccaccggc
      481 cgtcttcatg ggcctgcagc gaccacaatt accttgatca tgtattcacc tcgatgagtg
      541 cttttctgcc tcggcccccc ggggcggcaa aatattaacg ccaccttcaa gatcgtgcaa
      601 acatgcatgt gggatgcggt tcaggggagt atccgcgcaa cccggcagat caagatcgca
      661 aacactgacc gatcgcgtga gcaaggatgt cgcagccgcg ggtcggtggg tgacaatgcg
      721 cccagcaaat actgcctacg ccagcggttc ggtcgctacg ccgtagcact gctgatagcc
      781 ggcgacctgc tcgcgtagct gctccgcgtc cagtccggtg tcggcccagc tgtaacggcc
      841 gcccccgccg tcgccgggat tggccgccag atgctctcgc atccgctgct cggcgatggg
      901 ggtcaactgt cggtccagcc ggccgtacag cgacctgatc gtcgcgaacg ggtcgcgcat
      961 gaagtcggtg aactgcacgt cgatcacctg gccgggaggc agcgattcgc gcagtgtcat
     1021 cccgcgctgc aatccgacca cgatttcctc gcacgactgc accgcgcagt cggcgaccgt
     1081 tgtctcgtcg gaggccatgc ggcgcaggtg atttgtcagc gcactgatcg acgagatcac
     1141 cttcaacggg tctcggtgcg tctgcacgat taccgcgccg gggtacgtct ccaccaacgt
     1201 atcgagtgtc cacagatgtg ccggtgactt cagcagccac tggcctttga cgccggattg
     1261 caagtgttgc aggaagatcc ggtggaatcg ataagcctga cgatggtccg cctcatacag
     1321 tagccacttg tagtagccgg gcagtcggta ctggaccgag aaaatcatgc tgcagaactg
     1381 cgatgcggtg atccgcacac actcctgtcc gacctgcgca cccatcgggt gccagctcat
     1441 gaacccggga atgatctgct cgctcatctc caaacttgtc tgggtttcgg cgatgcgcgg
     1501 gtcggtgtcg taggtgtcgg gctgtggcac tggaagcgga gcgtcgacct cccacgtcag
     1561 cggcggtcgc agctcggggt cctgagccag caggtcgaac aggatcgtgg tgccggtgcg
     1621 cggctgcccg acgatgaaga tgggccgctc gatcctctcg ccggcgatcg cgggatgggc
     1681 cttgcgccac ctgacgatct gaagtcgatt cttcaaggcg cgcacgatgt cggaggccgc
     1741 gatctccacg ccgagatcgt tgagccgggc ctcctcgacg aacccctcgc acagccggcc
     1801 aagtccgtcg cgccaggtct ggttctcgtc gaactccgcg ccgaagtcgt cacagcccgc
     1861 ccactgacat gcctggtcga tcaacgcagc cggctggaac cggtcggctg tgctcaccgt
     1921 gtcgcctcac cgattcgcac gtcgacggac accttcggcg ggtgcgggtt gtccagccac
     1981 cggaacacca cgaagccacg gtgccggccg ccggtgtcca gccagtggcc atggccggag
     2041 tccttggcgc cgacggcgac acgcaccacg ccgtcggcat cgggggtgac acccttgttg
     2101 gtgaccgagc tgtgcctgcg acgcggttcg aggcattcgt gccagatgtt ctccagcgtg
     2161 atgttccagt agcgggtgtc cgggggttcg aactcgagta ccagtgactc gtcgtcgccg
     2221 agcgcgaagc tgccgagcat atacaggttg tccggtgtgg tgtcggcctc tccgaggtcg
     2281 gccgcctccg acgtgacgag ggtgttcggc tgccgcatca gctcgggctt gatggtgcgg
     2341 tgcagggtgg tcagcttcat gatggtccac gccatcgcgg aaaactgttc tgcggcttcg
     2401 tcatcggaca ccttctgcgg cggctgtgga gtggtgagcg gatcgatgtg cagcgtggcg
     2461 gggtgctcgc tgtgcgcgtc ggcgatgtac tcgcggacga cgatcgacgt cgcatcctcc
     2521 ggtatgggca cccactgcgc gccgtcgagt tcggcaggcg acggctccgt caccgagaag
     2581 accagcgcca acctgccgcc gtcgagcttc atgtcggtgt cgctgacgta cgccgccatg
     2641 cggcgcggtg tcatgccggt gcccgccagc acctgcacgc cgagataggc ggtggtgcct
     2701 cgtgttccgg tgacgcgata gccgcggtcg ccgcgaatca tcgcgagcag gtacctgccg
     2761 tccggattcg ggccgccgac aaggcgggtg ggggagcaca tgtcgaagaa ccacggcagc
     2821 tccgggtcgg cttccaccgt cagctccgag cacagcgcgg tgacacggga gatgacgccc
     2881 agaccctcga gaagttcgcg ttcgttctcg gcgtccgcgg tcacgatgtt cgtgatgtcg
     2941 ccgatcatct tctgcacgaa ggcccacgcc tcggctgtct tgggagccaa cgcctcgtca
     3001 ttcgtcacgt tgagacaact atcagtacag agcctgtctg ttggggcgaa tcagctattt
     3061 gacgacgtcg accggcgcgc cgcctcctgg gcgagctgga cccgggagga cagcccgagc
     3121 ttcgtgtaga cgcgggtgag gtgagactgc actgtccgcg gagagatgaa cagccgggtg
     3181 gcgacgtcct tgttgccgag cccctcgctg acgagccgca cgacatccag ctcggccggg
     3241 gtcaatgagg cccagccgct cgacggtcgt ctgcgttcgc cacgcccgcg ctgcgcgtag
     3301 gcgatcgcct cgtctcgcga cagggcggcg ccctctgccc atgcagcgtc aaagtcggcg
     3361 tcctccatcg catttcgaag cgctgccacc gatgcgtcgt attcggcttg gtagatgcgc
     3421 cagcgaacct cgccagtacg ctgccggatg cccgcagccg caccgaagag ccgagccgct
     3481 tcgcggtgtc tggcgccatc gcccgcgacg cccgccagga cgtccagaat gtcgggaacg
     3541 ccgagataag ctccgacatc cacccaagtt gcaagggcgt catgagcgtc tcgctcggcc
     3601 tcttccggct tttgttcggc gatcgcgatt cgtgcgcgtg tcgcgagtgc cgctccaagt
     3661 gtccacccct ttactgcggc gatagtcgtg tcggcccagc gtcgggcgcc gtccagatcg
     3721 ccgcgagcca gttccagcgc tgcgatgaag acactgttga tcgacgccgt cccttgaagc
     3781 tggccaatca gaggccacgc ggcatcagcc gcatcgactg cgagtgcgac gtcgcccgcc
     3841 gcaaggtggg tcaccatcag cgctatgtag ctgccaccac ggaagacgtc accgagttcg
     3901 gcaccggcct cgacgcctgc ctcggcggcg actcgcgcgg cctccgtatc acctgcggca
     3961 atgagagcgt ggggcatcat gagaagcgcc gcgacccgag agatggtgtc gtgatcggtc
     4021 tcgctttcgg ctatgacggc gcggaactcg atgatcgcgt cggcaatgtc actctcaaac
     4081 acctgcgcgc ctgcgagccg ccaccggcac tgccgagagc cgaatctgtc accgattgca
     4141 tcggcgacgt cgcggccctc cgcagcggcc gcgtgcaacg ccctcacatc gcccgccacc
     4201 aatgcggcat gtgcctgctg gcccaggagc tgggacagcc gccactggtc acccagctcc
     4261 cgggtcaggc cgagggcttc cccgaagtag cgcggtgctg cttcggcgtc gtagacggct
     4321 gcactgcaac atgcggtaag ggctcgggcc atcagggcgg gatcgtcgtg ttcacgcgcg
     4381 attgcgaggg cttgttcggc gtcaccaagg ctgtaggtgt tgacccaggc ctcgagcacg
     4441 accttgtctg caagtacccg cgcgcgcatg ggatgtgcca gatcgcttcg gctcgccttc
     4501 tcgtcgccca gcgcagcatc gaaccaggcc agcccctcct ggattcggcc ccgtgacaac
     4561 cacagcggtt ggagggagca ggccagctcc aacgcgctcg caatgtcgcc cgattcgagg
     4621 ctccagccga atgcggcccg aagattgtcc atgtcgtcat cggcctgctc gacgcgaagc
     4681 tggtaatcgc ttctggccgg cgagtcgagc aacgccgcca tcgagttgta gtggtcgcgg
     4741 tggcgcgacc ggactgcttc ggcctcgccc gactcgccga gcttctcctg cgcgtattgg
     4801 cgcaccgtct cgagtagccg gtaccgcata tggcccttgg tattttcggc agtcaccaat
     4861 gacttgtcga ccagcagggc tagttgatcg aggacttgat agcgctgtac ttcagcgtca
     4921 ccggcaacgg tctgggccgc gtcgaggtcg aacccgccga gaaataccgc gagccgtcgg
     4981 aacaggacgc gctcggtttc ggtcagcaga gcatgagacc agtccaccga tgcgcgcagc
     5041 gtctgctggc ggcgtaccgc tgtccgtgcg ccaccggtca agagacggaa gcggtcgtgc
     5101 agtccgccga caatctcctc gagcgagagt gcacgcacgc gcgcggcggc cagctcgatc
     5161 gccagcggca taccgtcgag cctcttgcag atctcggtca cggcaggggc gttgtccgcg
     5221 gcgatggtga actcgggtcg gacccggcgg gcgcggtcga cgaacaactc gagcgcctcg
     5281 tcgaccagcg acaacgacgg cacctggaat gtcacctcac ccggcacacc cgtcgactcc
     5341 cggctggtgg caagtatctg cagcgcagga catgcggaca gcagctcggc cgccagcgat
     5401 gcggccgcgt cgagcaggtg ctcgcagttg tccaacacca tcaatgtctg ccggtcgccg
     5461 acgaacttcg tgagcgattc cgtcatcgaa cggcccggct gatcgggcag gccgagcgca
     5521 cgggcaacgg ccaccgggac cacggcctcg tatgtgaccg gtgacaggtc gacataggag
     5581 acaccatcgc ggtactgggg cgcgatgtcg gccgcgacct ggatcgccag ccgcgtcttg
     5641 cctgcaccgc cggcgcccgt cagcgtcaca attcggttgt cggacaacag cgcgcgtatc
     5701 tccgcgatct gcggtgcgcg gccgacaaag ctcgttaatt gtcccggaag gctgtgcttt
     5761 tcggcgtttc tggtcacgcg caggggcggg aagtcgttgc tgaggtcggg gtggcacagc
     5821 tgcgtcaccc gttcgggcct tggtagatcg cgcagctcat gggatcccag atcggtcagc
     5881 caggcgccgt ccggcagcca gtcggcgacc agctgctcgg tggcacccga cagcacggtc
     5941 tggccgccgt gcgcgagatc gcgcaaccgg gcggttctgt tgatcgtcgg accgatgtag
     6001 ttgccctcgt cgcgcagctg cacctgcccg gtgtgcacac cgatgcgcag ccggatcggc
     6061 gccagcg
//
LOCUS       DASYLX010001034         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2590706,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001034 DASYLX010000000
VERSION     DASYLX010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590706"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGP27_24270"
     CDS             complement(<1..205)
                     /locus_tag="VGP27_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HEV7854449.1"
                     /translation="MDANTVTLDRALDETRTGDIWLFRGDSRPDRAIQTMTNAPVNHV
                     GMTVAIDDLPPLMWHAELGDKLQD"
     gene            233..400
                     /locus_tag="VGP27_24275"
     CDS             233..400
                     /locus_tag="VGP27_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854450.1"
                     /translation="MHSIWKWVGLAGAAGVVAGGVLVARDQRRRNSFTPDDIRAKLHQ
                     RLAETEGRKIN"
     gene            complement(393..1385)
                     /locus_tag="VGP27_24280"
     CDS             complement(393..1385)
                     /locus_tag="VGP27_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854451.1"
                     /translation="MAATSGGAMLRLSEIQAWDTEHLTAAARWWETTATRWEDGLSEV
                     VRHSYTPGGSIWEGAAATAAQEGALSDRRKVSGAAQHLRTTSTAARAGADALASERQR
                     VLAAVNAARAAGFLVGDDLSVSYDDDGTPASAAKRAQAASMAREIWQSARQLAMTDRR
                     VADQIERSSNGIQSLNFGLGGDPPLTPADGRGPHNAEDVFRIVDPLPPGKQPNVRELP
                     TSDQIRALYDWLAQNGLPAPPSSYKGTESVLPDGTRIGIREDSLSGGTTVDINFGDGR
                     EGMKVHLPRDGDEPQPATASGNPGFWETVGGIGVAILGGIGWVGSHLAHPLSPS"
     gene            complement(1388..1675)
                     /locus_tag="VGP27_24285"
     CDS             complement(1388..1675)
                     /locus_tag="VGP27_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854452.1"
                     /translation="MSSVHVDPAALHAAAGKIDGVAATFTGTGAPAVPMKSAQSTTAA
                     VNAALVATATAEKTIATRLRSTASALASGGTAFATTEHASTAAIAAVPDAR"
     gene            1761..2480
                     /locus_tag="VGP27_24290"
     CDS             1761..2480
                     /locus_tag="VGP27_24290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854453.1"
                     /translation="MSWARDEVLFRAQSGLLGLRAVQNLVCRESRSANGQATPEAIQL
                     ALVDLVRSLVEEGFAVVGDRTQAGFVPWTIPVVDGLDGREGWLRLTEAGRVAARDVPV
                     GAVGDLPDGAAKQWDWPFAQAAARVLVYGTIDWVELGQIHWRVKEVSPDVPIDVVQQR
                     TLELIAELVRGGLVVVGSIDTGAYGFVAWECSVEEALSRIRAVYVDRFDDESAWEWFC
                     LLELTRRGTVLAREIEAKAQN"
     gene            complement(2490..3278)
                     /locus_tag="VGP27_24295"
     CDS             complement(2490..3278)
                     /locus_tag="VGP27_24295"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7854454.1"
                     /translation="MTGVQIAAYVLAAVAVAEAVALVVLWVQLTRARREADELRGRVD
                     TRNMLLSGGREAVKTMWETANIIRKEGFGAAVRSSIEDLADWAEVERPDLARLAPDGN
                     VAILFSDIEESTALNERIGDRAWVRLIGRHDKLIRRHVQSHRGHVVKSQGDGFMVAFA
                     DPEQAVRCSIDVQRALSKRPNGIRVRIGIHTGKSVRRGDDLFGRNVAMAARVAAQADG
                     GQTLVSEPVRESVGGKDGIGFDDGQDVELKGFSGRHRLYAVEPA"
     gene            3304..4257
                     /locus_tag="VGP27_24300"
     CDS             3304..4257
                     /locus_tag="VGP27_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854455.1"
                     /translation="MKPHPPYYESRFIRGNDPDRARALWIRSTLLLPTSGAPVADSWM
                     MVFDPDGSGNRALKQPYPIDQSDYHYDTWSARIGATSIDDRSVCGSISGSGRSARWDV
                     QITPGDEAPVKLLTDRGYKAKFPTAKTMVRHPLARFDGSVELDDVSLQLDGWTGSVNH
                     NWGRRHTPAYAFGQVCGFDGAPRSSLEVVTAHAAVGPVSLPAATLLVLRHDGREVAVR
                     SVLATRHTRGEYRPFSWSFGARVDDVTVDGEITAQPREVIGLTYTDTDGESKYCYNSA
                     LATCRIQLSGSGFATTELTATRRAMFEVLTDTRNASVPLLA"
     gene            complement(4350..>4533)
                     /locus_tag="VGP27_24305"
     CDS             complement(4350..>4533)
                     /locus_tag="VGP27_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007726173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HEV7854456.1"
                     /translation="QVHRQVSDGDLVMNERTDVMRRKGGEPIPLPVTGVFEIENGRIK
                     AWRDYFDMATITSAFG"
BASE COUNT          690 a         1475 c         1551 g          817 t
ORIGIN      
        1 tgtcttgcag cttgtcgccg agttcggcgt gccacatcaa cggcggcagg tcgtcgatcg
       61 ccacggtcat cccgacatgg ttgaccgggg cgttggtcat cgtctggata gcgcggtccg
      121 ggcgggagtc gccgcggaac agccagatgt caccggtgcg ggtctcgtcg agggcgcggt
      181 cgagggtgac cgtgttcgca tccacgaggg cagcttaggc acactgacag gcatgcacag
      241 catctggaag tgggttggac tggccggtgc cgccggagtc gtggccggtg gcgtgctggt
      301 cgcacgcgat cagcggcgtc gcaattcgtt cacgcccgac gacatccggg ccaagctgca
      361 tcagcggttg gccgagaccg aaggccgaaa gatcaactag ggctgagcgg gtgtgcaagg
      421 tggctgccga cccagccgat tccaccgagt atcgcgacgc cgatcccacc tacggtttcc
      481 cagaagccgg ggtttccgga tgcggtggcg ggctgcggct cgtcgccgtc cctgggcagg
      541 tgaaccttca tgccctcccg cccgtcgccg aagttgatgt cgacggtcgt tccgccggac
      601 aagctgtctt cacggatgcc gatccttgta ccgtcgggca gcacgctctc ggttcccttg
      661 taactgctcg gcggagccgg cagaccgttc tgagccagcc agtcataaag ggctctgatc
      721 tgatcagatg tcggtagttc acggacgttg ggttgcttac cgggtggcag cgggtcgacg
      781 atcctgaaaa cgtcctcagc gttgtgcgga cctcttccat ctgcgggcgt caacggaggg
      841 tcgccgccga gtccgaagtt caggctctga atgccgttgg acgacctctc gatctggtcc
      901 gccacccgac ggtccgtcat ggccagttgt cttgcgctct gccagatctc gcgcgccatg
      961 gatgcagcct gcgctcgctt ggctgccgaa gcgggcgtcc cgtcgtcgtc gtaggacacg
     1021 ctgaggtcgt cgcccacgag gaaacccgcc gcccgggctg cgttcaccgc ggccagcacc
     1081 cgctggcgct ctgaggcgag tgcgtcggcg cctgcgcgcg cagccgtcga cgtcgtacgg
     1141 aggtgctggg cggcgccgct caccttcctc cggtcggaca gtgcgccttc ctgggcggcg
     1201 gtggccgcgg cgccttccca gatgctcccg cccggcgtgt acgagtggcg aacgacttcc
     1261 gacagcccgt cttcccagcg ggtggcggtc gtctcccacc accgcgcggc cgcggtgagg
     1321 tgttcggtat cccacgcctg gatctccgat aatcgcagca tcgcgccgcc ggaagtggcg
     1381 gccattccta tcgggcgtcc ggtacggctg cgatggcggc tgtggacgcg tgttcggttg
     1441 tggcaaaagc ggttccgccc gaggccagcg cagacgcggt tgagcgcagg cgggttgcga
     1501 tggtcttctc cgctgtcgct gtggcgacga gggcggcgtt gacggcggcc gtcgtggact
     1561 gcgcactctt catcgggacc gcaggcgcgc ccgtccccgt gaacgttgcc gcgacgccgt
     1621 cgattttgcc ggccgcggca tggagcgccg cgggatcgac gtgaactgac gacatctgac
     1681 aaccgtagcg ttgtccggga gagggtcgct gcacgaattc gatgcaggaa atacgccgaa
     1741 agttcggtag cgtcggcgcc atgtcatggg cgcgcgatga ggtgcttttt cgggcgcaat
     1801 ccggtctgct cggcctgcgc gcagtgcaga acctggtatg tcgggagtct cgcagcgcca
     1861 acgggcaggc gaccccggag gccattcagc tggcactggt ggatctggtg cgttcgctcg
     1921 tcgaagaagg gtttgccgtc gtcggtgacc gcactcaggc cggattcgtg ccgtggacaa
     1981 tccctgtcgt cgacggactc gacggccggg agggctggct gcggctgacc gaggcgggcc
     2041 gcgtagcggc tcgggatgtg cccgtcgggg ccgtcggaga cctaccggac ggcgctgcca
     2101 aacaatggga ttggccgttt gcgcaggcgg cggcgcgcgt gttggtctac ggcaccatcg
     2161 attgggtcga actgggtcag atccactggc gtgtcaaaga ggtcagccca gatgtgccca
     2221 tcgacgtagt gcagcagcga acccttgagt tgatcgccga gctggtgcgc ggcgggctgg
     2281 tggtggtggg ctccatcgac accggggcat acgggttcgt ggcgtgggaa tgctcggtgg
     2341 aggaggcgct gagccgcatc cgcgccgtgt acgtcgatcg gttcgacgac gagtccgcgt
     2401 gggaatggtt ctgcctgctg gagttgaccc gcaggggcac agtgctcgcc cgcgagatcg
     2461 aagccaaggc gcagaactga gcggcaagct caggcgggtt cgaccgcgta cagccggtgg
     2521 cggcccgaga agcccttgag ctcgacgtct tggccatcgt cgaagccgat gccatccttg
     2581 ccgccgaccg actctcgtac cggttcgctg accagtgtct ggcccccgtc ggcttgtgcg
     2641 gcgaccctcg ccgccatcgc gacattgcgg ccgaacaggt cgtcgccgcg ccgcaccgac
     2701 ttgcccgtgt ggatgccgat gcgtacccgg attccgttgg gccgcttact gagtgcgcgt
     2761 tgtacgtcga tgctgcaccg caccgcttgc tccggatcgg cgaaggcgac catgaacccg
     2821 tcgccctgac tcttgacgac gtgaccccga tgactctgca catgccgtcg gatcagcttg
     2881 tcgtgtctgc cgatcagacg gacccatgcc cggtcgccga tgcgttcgtt gagcgccgtc
     2941 gactcctcga tgtcggagaa caggatcgcc acattcccat cgggggccag acgagccagg
     3001 tcgggccgct ccacctcggc ccagtcggcc aggtcctcga tggacgagcg gacggctgct
     3061 ccgaagccct ccttgcggat gatgttcgcc gtctcccaca tcgtcttcac ggcctcgcga
     3121 ccgccggaca acagcatgtt gcgggtgtcg acgcggccgc gtaactcgtc ggcctcgcga
     3181 cgcgcgcggg tcagctggac ccaaaggacg acgagcgcga cggcttctgc gacggcgacg
     3241 gccgccagga cgtaggcggc gatctgcact ccggtcaccg cctaagtatt ggatgctggt
     3301 cgggtgaagc ctcacccgcc ctattacgag agcaggttca tccgcgggaa cgaccccgat
     3361 cgcgcgcggg cgctgtggat ccgctcgacc ctgctgctgc cgacgtcagg cgcacccgtc
     3421 gccgactcct ggatgatggt tttcgacccc gacggctccg gtaaccgggc gctcaaacag
     3481 ccgtatccga tcgaccagtc cgactaccac tacgacacgt ggtccgcccg gatcggcgcc
     3541 acctccatcg acgaccggtc ggtatgcggg tcgatcagcg gtagcggccg gtcggcgcgg
     3601 tgggatgtac agatcacgcc gggcgacgag gcgccggtca aactgctgac cgaccggggc
     3661 tacaaggcga agttcccgac cgccaagacc atggttcgcc acccgctcgc gcggttcgac
     3721 ggcagcgtcg aactcgacga cgtgagcctg cagttagacg gctggaccgg cagcgtcaac
     3781 cacaactggg gtcgtcgcca tacccctgcc tacgcgttcg gtcaggtgtg tgggttcgac
     3841 ggggcaccgc ggtcgagcct ggaggtcgtg accgcacatg cggccgtcgg tccggtctcg
     3901 cttcccgcgg ccacgcttct ggtgctgcgc catgacgggc gggaggtcgc cgtgcgctcg
     3961 gtgctggcga cccgccacac gcgcggcgag taccggccgt tcagctggtc gttcggcgcc
     4021 cgcgtcgatg atgtgacggt ggacggagag atcaccgcgc agccgcgtga ggtcatcggg
     4081 ttgacctata ccgataccga cggagagtcg aagtactgct acaactccgc gctcgcaaca
     4141 tgtcggatcc agctgtccgg cagcggattt gcgacgacag aactcaccgc cacccgccgc
     4201 gcgatgttcg aggtactgac cgacacccgc aacgcctcgg tgccgctcct ggcctagttt
     4261 tctcggcgag cagacgctaa atcgcgtgct tcggtgcaat tttgcatctg ctcgccagga
     4321 gaggtgtcgt gccagggagg ccagcccaac tagccgaacg cgctggtgat cgtcgccatg
     4381 tcgaagtagt ctcgccacgc tttgatccgg ccgttctcga tttcgaagac cccggtgacg
     4441 ggaagtggga tcggctcgcc gcctttgcgc cgcatgacat cggtgcgctc attcatcacc
     4501 aggtcgccgt cgctgacctg cctgtgcacc tga
//
LOCUS       DASYLX010001035         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_178828,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001035 DASYLX010000000
VERSION     DASYLX010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178828"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGP27_24310"
     CDS             <1..930
                     /locus_tag="VGP27_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7854457.1"
                     /translation="LLNEILDLSKVEAGQMMLDPSTFSVPSALEYTLAMVRERAAAHA
                     IKISVDIADDVADIEADELRFKQVVLNLLSNAVKFTPDGGSVSVTAYRDDAELVVAVT
                     DTGIGVPPEDQERIFDSFQQGGRGPAREEGTGLGLTLSRRIVGLFGGRMWLKSTVGVG
                     STFAFAIPAASRATEAATMQHDGEFPVVVLVDDDRASLDLMSAYLDDAPVQVIRTSDG
                     VSGLELIRKVLPAAVVLDIKLPRLDGWQVLTELKASEDTAAIPVVIASVVDDRPRGIA
                     LGADAQLLKPVSRDDLIGALRSVGVDGKRATGG"
     gene            923..2038
                     /locus_tag="VGP27_24315"
     CDS             923..2038
                     /locus_tag="VGP27_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7854458.1"
                     /translation="MDDPVTVLAVDDQSVNLRLLDAVLTPRGHRVLTASSGADGLGLL
                     ETEDVDLVLLDVVMPEMDGYEVCRRIRSNESTEFLPVVMITASGSEQRLNALLAGADD
                     FITKPFDQSELLARVASLARIKRYHDTIRRQAAELAKWNTELETRVAAQVFELERTNR
                     LRRFFSPQLADVVVGDEHLLESHRREIVVLFADLRNFTPFAETSEPEEVMGVLAEYHK
                     AMGTLVHEYQGTLERFTGDGIMVFFNDPIPHDDAAERAVRMALRIRDAVKDLAVGWRR
                     RGYDLALGQGIAQGFATLGRIGFEGRFDYAAIGSVTNLAARLCSDAGPWQVLVTDRIL
                     AAIENVADAEVVGDVAPKGFSRTVRVHNICAMKEQST"
     gene            2035..3609
                     /locus_tag="VGP27_24320"
     CDS             2035..3609
                     /locus_tag="VGP27_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEV7854459.1"
                     /translation="MTQLQAPARTLGELDEDDRYRAFDDLQLSMGHAWDSIQKNLEDE
                     SVVVVPSISIERTTASTGTVIQAMEERALFLLLLLRQPRLQMIYVTSQPISEAIIEYY
                     LGLLPGVIPSHARARLTLVPVGDASATSLSAKLLARPRLLREIRSLIPDPERCHLIPY
                     NTTPLERDVAISLGIPMYGADPRLVDLGSKTGCRRMFEECGVVYPVGAEDLHSVDDIV
                     AAVQGMRRRRPSITLAIVKINEGVSGSGNASLDLNGLPEPGAPDEATVIAERLRSLVP
                     ESEKLSVDAFLTAFEKDGGIVEERITGVSLESPSVQMRALPDGTVELLSTHDQLLGGA
                     SGQKYLGCVFPADQGYAAAIAEPAMVVGRHLAQRGVLGRFAVDFVTVKDETGAWTPYA
                     IELNLRKGGTTHPFLTLQFLCGGSYDGQRGVFVTPEGNAKYLVATDHLEDDRLKVLTV
                     EDLFDVVVQNGVHFDQSRRTGVVFHMISCLTECGRIGMTAVGDSPEEAWRLYQEAEAI
                     LLREADLALQEEAVVH"
BASE COUNT          648 a         1193 c         1243 g          615 t
ORIGIN      
        1 ctgctgaacg agatcctcga cctgtccaag gtcgaagcgg gccagatgat gctcgacccg
       61 agcaccttct cggtgccgag cgctctcgag tacacgctgg cgatggtccg cgagcgcgcc
      121 gccgcgcacg ccatcaagat ctcggtcgac atcgccgacg acgtcgcaga catcgaagca
      181 gacgagctgc ggttcaagca ggtcgtcctc aacctgctgt ccaacgcggt caagttcaca
      241 ccggacggcg gcagcgtgtc cgtcactgcc taccgggatg acgccgagct cgtggtcgcg
      301 gtgaccgaca ccggcatcgg tgtaccgccg gaggaccagg agaggatctt cgactcgttc
      361 cagcagggtg gccgcgggcc tgccagagaa gaggggaccg ggctcggatt gacgctgtca
      421 cggcggatcg tcgggctctt cggcggccgg atgtggctga aaagcactgt cggcgtcgga
      481 agtacgttcg ccttcgcgat tcccgcggcg tcacgcgcca cggaggcggc cacgatgcag
      541 cacgatggcg agttcccggt cgtcgttctc gtcgacgacg accgggcctc gctcgatctc
      601 atgtcggcct acctcgatga cgcgcctgtg caggtgatcc gtacctccga cggcgtcagc
      661 ggtctggagc tgatccgcaa ggtactgccc gccgcggtcg tcctcgacat caagctgccg
      721 cggctcgacg gctggcaagt gctgaccgaa ctcaaggcgt ctgaagacac cgccgccatc
      781 cccgtcgtga tcgcctcggt cgtcgacgac cggccccgag gcatcgccct cggcgcggat
      841 gcccagttac tcaagccggt cagtcgcgac gacctgatcg gcgcactgcg aagcgttggc
      901 gtcgacggga agagggccac cggtggatga tcctgtgacg gtactggcgg tggacgacca
      961 atcggtcaac ctgcgcctgc tcgacgcggt cctgacaccg cgcggacatc gcgtcctcac
     1021 cgcatcgtcg ggggccgacg ggctcgggtt actcgagacc gaggacgtcg atctcgttct
     1081 gctcgatgtc gtgatgccgg agatggacgg ctacgaggtg tgccgccgga tccggtccaa
     1141 cgagtcgacg gagttcctac cggtcgtgat gatcacggcc agcggtagcg agcagcgtct
     1201 gaacgccttg ttggccggcg ccgacgactt catcaccaag cccttcgacc agagcgaact
     1261 actggcccgg gtggcgtcgc tggcacgcat caagcgatac cacgatacga tccggcgcca
     1321 ggccgccgaa ttggccaagt ggaacaccga actcgagaca cgcgtcgccg cacaggtatt
     1381 cgagctggag cggacgaacc ggttgcgccg cttcttctca ccgcaactgg ccgatgtggt
     1441 cgtcggcgac gagcacctcc tggaaagcca tcgccgcgaa atcgtggtgc tcttcgccga
     1501 tctgaggaac ttcaccccgt tcgccgagac cagcgagccc gaggaggtga tgggtgtgct
     1561 cgcggagtac cacaaggcga tgggcacgct ggtgcacgaa taccaaggca cactggagcg
     1621 gttcaccggc gacgggatca tggtgttctt caacgacccg atcccgcacg acgacgccgc
     1681 cgagcgcgcc gtccggatgg cattgaggat ccgcgacgcc gtgaaagacc tcgccgtcgg
     1741 atggcgaagg cggggctacg atctcgccct cggacagggg atcgcccagg gcttcgcgac
     1801 gctcggccgg atcggcttcg aaggccgctt cgactacgcc gcgatcggca gtgtcaccaa
     1861 cctcgccgcg cgcctgtgct ccgacgcggg gccctggcag gtgctggtca ccgaccgcat
     1921 cctcgccgcc atcgagaacg tggccgacgc cgaagtcgtc ggcgacgtgg cacccaaggg
     1981 attcagccgg accgtgcgag tccacaacat ctgcgcaatg aaggagcaga gcacatgacg
     2041 caactacaag cccctgcccg aacactgggt gagctcgacg aagacgaccg ctaccgcgcg
     2101 ttcgacgacc tgcaactgtc gatgggtcac gcgtgggatt cgatccagaa gaacctggag
     2161 gacgagtccg tcgtcgtcgt cccgtcgatc agcatcgagc gcaccacggc ctcaaccggc
     2221 acggtcatcc aggcgatgga ggagcgcgcc ctgttcctcc tgctgctgct tcgtcagcca
     2281 cggctgcaga tgatctatgt gacgtcgcag ccgatttcgg aggcgatcat cgagtactac
     2341 ctcgggctgc tgcccggcgt catcccgagc cacgcccgcg cgcggctcac cctggtgccg
     2401 gtgggcgatg cgtcggcgac gtcgctcagc gcgaaactgc tggcgcggcc acggctgctg
     2461 cgggagatcc ggtcactgat tccggaccca gagcggtgtc acctgattcc gtacaacacc
     2521 acgccgctcg agcgtgatgt cgctatctcg ctgggcattc cgatgtacgg agcggatccc
     2581 cgtctcgtcg atctcggcag caagaccggc tgtcgtcgga tgttcgagga gtgcggtgtg
     2641 gtgtatccgg tgggcgcgga ggaccttcac tccgtcgacg acatcgtggc agccgtgcag
     2701 ggcatgcggc ggcgacggcc gtcgatcacg ctggcgatcg tcaagatcaa cgaaggggtg
     2761 tcgggctcgg gcaacgcgtc gttggatttg aacgggctac cggaaccggg tgcaccggat
     2821 gaggcgacgg tgatcgccga gcggttgcgg agcctggtgc cggagtcgga gaagctgtcc
     2881 gtcgacgcgt ttctcaccgc gttcgagaag gacggcggca tcgtcgagga gcggatcacc
     2941 ggcgtctcgc tggaaagccc gagcgtgcag atgcgggcgt tgccggacgg cacggtcgaa
     3001 ctgctgtcca cgcatgacca attgctcgga ggagccagcg gccagaaata tctgggctgc
     3061 gtgttcccgg ccgaccaggg gtacgcggcc gccatcgccg aacccgcgat ggtcgtcggg
     3121 cgccatctcg cgcagcgggg tgtattgggg cgcttcgcag tcgatttcgt gaccgtgaag
     3181 gacgaaaccg gtgcgtggac gccgtacgcg atcgagctga acctccgtaa gggtggcacc
     3241 acccatccgt tcctgaccct gcagttcctg tgcggcggca gttatgacgg ccaacgcggc
     3301 gtcttcgtga ccccggaggg caacgccaag tacctcgtcg caacggacca cctcgaagac
     3361 gaccgcctca aagtgctgac cgtggaggac ctcttcgatg tggtggtgca gaacggcgtg
     3421 cacttcgatc agtcgcgacg taccggagtg gtattccaca tgatcagttg cctgaccgag
     3481 tgcggccgca tcggcatgac cgccgtcggc gacagtcccg aggaagcatg gcggctgtat
     3541 caggaggcgg aggccatcct cctacgcgag gcggacctgg cgctgcagga ggaggcggtc
     3601 gttcactaga ttggacgcga cggttggtag ggccgccatt gtccttccgg ttgagccaga
     3661 cggtcagcga tcacccgcag cgccttcacg ttgtggccg
//
LOCUS       DASYLX010001036         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_617268,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001036 DASYLX010000000
VERSION     DASYLX010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617268"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="mftF"
                     /locus_tag="VGP27_24325"
     CDS             <1..695
                     /gene="mftF"
                     /locus_tag="VGP27_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209156.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HEV7854460.1"
                     /translation="VLKEVGGFDETLKSGEDVDLCWRFVEAGARLRYEPIATVAHEHR
                     TQLREWFVRRAFYGESAAPLSVRHPGKTAPLVISGWTLMVWVLLTMGSTFGYLASMVV
                     ATITGRRIADSLGSVDTEPKEVAVVAAHGLWSGALQLAAAICRHYWPIALILAMLSRR
                     CRNVVLVAAVIDGVVDWVKRHGNVDDDTKPVGLVSYVLLKRLDDIAYGTGLWTGVVRE
                     RHIGALKPQIRT"
     gene            740..2161
                     /gene="mftG"
                     /locus_tag="VGP27_24330"
     CDS             740..2161
                     /gene="mftG"
                     /locus_tag="VGP27_24330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="HEV7854461.1"
                     /translation="MLIIGAGSAGSVVAERLSRDESCRVAVVEAGPAPSDPAVSAQIT
                     DAVRMPIGSASSVVRHYASTLTADPKRIGHIMRGAVVGGSGAVNGGYFCRALPEDFAS
                     WSLPGWAWTDVLPHFRAVERDLDFVGPLHGSDGPILVRRVSEFDGCTASFVEAAAEIG
                     YPWVADLNGAAEGRPLPAGVGAVPLNINGGTRIGPGGAFLQPALDRDNLTLIADTRAL
                     RIRFDRDRAVGVDCAGPDGTVQLSADRIVLCAGAIGSAHLLMLSGVGPEAALTGAGIP
                     IVLDSPVGLHTSDHPEWVLPVDWTPTHDLPPLEAILTTPNGLEIRPYTRGFGAMVEGA
                     GGDPADRPHIGVALMHPRSRGRLRVLSDDPAVAPLIEHRYDSDPHDVAMLSAGAELAR
                     ELASASTRVGHASWSTSQHLCATAPMGVEGDTSAVVDERCRVRGVDGLWVVDGSVLPT
                     VTGRGPHATIVMLGHRAADFVVG"
     gene            complement(2057..3736)
                     /locus_tag="VGP27_24335"
     CDS             complement(2057..3736)
                     /locus_tag="VGP27_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854462.1"
                     /translation="MVDTVVRSDQDIDADLASLSKFGRFWLLTVAAVDVLLVISSMVA
                     LNAALPDIAVQTAATQTQLTWVVDGYTLVLACLLLPAGALGDRYGRRGALLVGLAIFA
                     LASLVPTVFDSPVQIIVARAVAGIGAAFIMPATLSLLTAAYPKSERNKAIGIWAGVAS
                     SGAIVGFMGSGLLLHFFEWQSVFYAFAVASVALFVCTCTIRTSRDETATPVDWVGAVL
                     IGGAVAIFVFGVVEAPARGWSHPLVWGCMSVGVLLAISFTVVELRRRHPLLDVRLFAR
                     PDFATGSVGITFLFFANFGFFFVVMQYMQLILGYSPLQTAWALAPVAVPIVALGATMH
                     LILPRLGLRLVVTFGFLLIAAGLFSMRYLEADSAYIELVWPFLITSAGIGLCVAPTTS
                     AIMNAVPTEKQGVASAVNDATREVGAAVGIAVAGSVLAAQYTKALSPGLGVFPEQIRG
                     AATDSLANAIEMAKQLGPQGPMLAKLAESAFLDAMDLSVIVISVIVTVVAVFIAVWAP
                     GRDGRQSRFVRGLTARRSADDEVGGPVPEHHDGGVGSAAGDGGKHRAVDHP"
     gene            3829..4491
                     /locus_tag="VGP27_24340"
     CDS             3829..4491
                     /locus_tag="VGP27_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854463.1"
                     /translation="MTGDRDDPRPARSRARLLGAATALLRTGGPNAVTVDAVTRAANV
                     ARATLYRHFPSGNDLLAAAFHALIPPAPTPPAEGLLRDRLIALVQAQAEMIAEAPITL
                     TAMSWLTLGGDLERLPGAPHRGASDSPEVQTLRERVAEQYAAPFEAILSSPQALDELG
                     EVDRTKAAALLLGPIALGKLSTLPDFDYREIAVCAVDGFLATHRKRGKGLSAASNEQA
                     GA"
     gene            complement(4457..>4742)
                     /locus_tag="VGP27_24345"
     CDS             complement(4457..>4742)
                     /locus_tag="VGP27_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854464.1"
                     /translation="GESVYLAYVSGNRDEDIFEEPFRFDVGRDPNKHLAFGYGVHFCL
                     GAALARMEMNSLYTELVPRLESIELAGRPQLSATTFVGGLKHLPVRYSLR"
BASE COUNT          833 a         1515 c         1603 g          791 t
ORIGIN      
        1 ccgtactgaa ggaggtcggc ggattcgacg agacgctgaa gtcgggggag gacgtcgacc
       61 tgtgctggcg gttcgtcgaa gccggtgctc ggctgcgcta cgagccgatc gcgacggtcg
      121 cccacgagca ccgtacgcaa ctgcgagagt ggttcgtgcg cagggctttc tacggtgagt
      181 cggccgcgcc gctttcggtg cgtcatcccg gcaagaccgc gccgctggtg atttccgggt
      241 ggacgctgat ggtgtgggtc ctgctcacga tgggctccac gttcggatac ctcgcctcga
      301 tggtggtggc cacgatcacc gggcgccgga tcgccgactc acttggttcg gtcgacaccg
      361 agccgaagga agtcgccgtg gtcgccgcgc acggcctctg gtcgggggcg ctgcagcttg
      421 ccgcggcgat ctgcaggcat tactggccga tcgccctgat cctcgcgatg ctgtcgcgtc
      481 gctgtaggaa tgtggtgctg gttgcggcgg tcatcgacgg cgtcgtcgat tgggtgaagc
      541 gacacggcaa cgtcgacgac gacacgaagc ccgtcggcct ggtcagctac gtactactga
      601 agcgcctcga cgacatcgcg tacggcaccg gtctgtggac cggcgtggta cgggagcgac
      661 atatcggcgc cctcaagccg cagatccgga cctgacgtcg ttgcatattg gctcgcctgc
      721 tcgtcgcgcg cgtaatgacg tgctgatcat cggtgctggt agtgctggat ctgttgtagc
      781 ggagcgactt tcgcgtgatg agtcctgccg cgtcgcagtc gttgaggcgg gtcctgcgcc
      841 gtctgaccct gcggtcagcg cccagatcac cgatgcggtg aggatgccga taggctccgc
      901 cagctccgtg gtgcggcact atgcttcgac gctgaccgcc gatccgaaac gaatcgggca
      961 catcatgcgc ggggcggtcg tcggcggatc cggcgccgtc aacggcggct acttctgccg
     1021 tgcgctgcca gaggacttcg cgagctggtc actgccgggc tgggcctgga ccgatgtgtt
     1081 gccgcacttc cgggctgtcg agagggatct ggacttcgtc ggcccgttgc acggatcgga
     1141 tggcccgata ctcgttcgcc gagtctccga attcgacggc tgtacagcgt ctttcgtgga
     1201 agccgctgcc gaaatcggat acccgtgggt cgcggatctc aatggtgcgg ccgaagggcg
     1261 accgctgcct gccggtgtcg gcgccgtgcc gttgaacatc aacggcggca cgcgaatcgg
     1321 gccgggaggc gctttcctgc agccagcact cgatcgtgac aacttgacgc tgatagctga
     1381 cacgcgagcg ttgcgcatca gattcgatcg cgatcgcgcg gtgggcgtcg actgtgccgg
     1441 accggacggc accgtgcaac tgtcagcaga tcgaatagtt ctgtgcgccg gagcgatcgg
     1501 gtccgcccat ttgttgatgc tgtcgggtgt cgggcccgag gcagcgctca cgggtgcggg
     1561 tatcccgatt gtccttgatt caccggtggg gctgcacacg agcgatcatc ccgaatgggt
     1621 gctgccggtg gactggacac cgacccacga ccttcctccg ctcgaggcga ttctgacgac
     1681 acccaacggc ctggagatca ggccctatac gcggggtttc ggtgccatgg tggagggcgc
     1741 aggcggcgac ccggccgacc gaccgcacat cggtgtcgcg ttgatgcatc cgcggtcacg
     1801 gggaaggctg cgtgtgctgt ctgatgatcc tgcggtggcc ccgttgatcg aacaccgcta
     1861 cgacagcgac cctcatgatg tcgccatgtt gagtgccggc gccgaactgg cgcgtgaatt
     1921 agcttcagcg tcaacacgag tcggccacgc tagctggtcc acgtcgcagc acctgtgcgc
     1981 gaccgctccc atgggcgtgg agggcgacac ctcggccgtc gtcgacgagc gctgcagggt
     2041 gcgcggcgtc gacgggctat gggtggtcga cggctcggtg cttcccaccg tcaccggccg
     2101 cggaccccac gccaccatcg tgatgctcgg gcacagggcc gccgacttcg tcgtcggctg
     2161 atcgacgcgc cgtcaaaccg cgaacgaacc tcgactgcct gccgtcgcgg cccggcgccc
     2221 acactgcgat gaagacggct acgacggtga cgatgacgga tatgactatg acagataggt
     2281 ccatcgcgtc gaggaaggcc gattcggcca gcttcgccaa catcggcccc tgcgggccaa
     2341 gctgtttggc catctcgatg gcgttcgcga gcgaatcggt cgccgcgccg cggatctgct
     2401 cggggaacac gccgaggccg gggctgagcg ccttggtgta ctgcgcggcg agcactgacc
     2461 cggcgacggc gatgccgacc gccgcgccga cttcgcgggt ggcgtcgttg accgccgatg
     2521 cgacaccctg cttctcggtt ggcacggcgt tcatgatcgc cgatgtggtg ggcgccacgc
     2581 ataatccgat gccggcgctg gtgatcagga acggccagac gagttcgatg taggccgagt
     2641 cggcttcgag gtaccgcatg ctgaacaacc ccgcggcaat cagaaggaag ccgaaggtca
     2701 cgaccagccg gagtccgagg cggggaagga tcagatgcat cgtcgcgccg agggcgacga
     2761 tcggcacggc caccggcgcc agcgcccaag cagtttgcag tgggctgtat cccaggatca
     2821 gctgcatgta ctgcatgaca acgaagaaga agccgaagtt ggcgaagaaa aggaaggtga
     2881 tgccgaccga gccggtggcg aagtcgggac gggcgaacaa cctcacgtcg aggagcggat
     2941 gtctgcgccg cagttcgacg accgtgaacg agatcgccag taacacgccg accgacatgc
     3001 aaccccagac gagggggtgg ctccatccgc gggcgggagc ctcgaccaca ccgaatacga
     3061 agatcgcaac ggcgccgccg atgaggacgg cgcccaccca gtccaccgga gtggcggttt
     3121 cgtcgcgtga ggtgcgaatt gtgcacgtgc acacgaacag tgcgacgctt gcgaccgcga
     3181 aagcgtagaa caccgactgc cattcgaaga agtggagcag cagcccggaa cccatgaacc
     3241 cgacgatcgc tccggaactc gcaacccccg cccagatgcc gatggccttg ttccgctcgc
     3301 tctttggata ggcagcggtc agcagggaaa gggtcgcagg catgatgaat gccgcgccga
     3361 tgcctgcgac agcgcgtgcg acgatgatct gaaccgggct gtcgaacacc gtcggcacca
     3421 gcgatgcgag cgcgaagatc gccaacccga caagcagggc accgcggcgt ccgtaccgat
     3481 cgcccagcgc gcccgcgggc aacagcaggc acgccagcac gagggtgtag ccgtcgacca
     3541 cccacgtgag ctgcgtctgg gtcgccgcgg tttgcactgc gatgtccggc agcgccgcgt
     3601 tgagcgcaac catcgaagag atcaccagca ggacatcgac ggctgcgacc gtcaacaacc
     3661 agaaccggcc gaatttcgat aaggaggcca ggtctgcatc gatatcctga tcggaccgca
     3721 cgacggtgtc gaccatgaac gtcactcgct ttcgatcggc tatttttgag actaacagtc
     3781 tcgctaggag accgacagtc ttgttcccgc agggaagggg gccaaaccat gaccggcgat
     3841 cgtgacgatc cgcgaccggc ccgatcccgg gcccgtctgc tgggcgcggc gaccgcgctc
     3901 ctgcgcacgg gaggtccgaa tgcggtgacc gtcgatgcgg tcacgcgtgc cgccaacgtg
     3961 gctcgcgcca cgctctaccg ccactttcca agtggaaatg atttgctggc agcggctttt
     4021 cacgcactga taccgcctgc cccgacgccg ccagcggaag gcttgctgcg agaccggctg
     4081 atagcgctgg tgcaggcgca ggccgagatg atcgccgagg ctcccatcac cctgaccgca
     4141 atgtcctggt tgaccctcgg cggcgacctc gagcggctgc ccggcgcgcc gcatcgcggc
     4201 gcatccgaca gtcccgaggt gcaaaccctg cgggagcggg tggccgaaca gtacgcggcg
     4261 ccgttcgagg cgattctcag tagtccgcag gcgctcgacg agctcggcga agtcgaccgc
     4321 accaaggcgg cggcattgct gctcggcccc atcgcgttgg gcaagctctc gacgctgccg
     4381 gacttcgact accgcgagat cgcagtttgc gccgtcgacg gctttctggc cacccatcgc
     4441 aagaggggca agggcctcag cgcagcgagt aacgaacagg caggtgcttg agtccgccga
     4501 cgaacgtggt cgcggacagt tgcggccgcc cggccaattc gatcgactcc aatcgcggta
     4561 ccagttcggt gtacaagctg ttcatctcca tccgcgccag ggcggcgccg aggcagaagt
     4621 gcacgccgta gccgaacgcc aggtgcttgt tcggatcgcg gccgacgtcg aaccggaacg
     4681 gctcttcgaa gatgtcctcg tcgcggttac ccgaaacgta cgccaggtag accgattccc
     4741 cc
//
LOCUS       DASYLX010001037         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2154623,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001037 DASYLX010000000
VERSION     DASYLX010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154623"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..693)
                     /locus_tag="VGP27_24350"
     CDS             complement(361..693)
                     /locus_tag="VGP27_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7854465.1"
                     /translation="MPPVLRNAVAFIQENADADIGLEDIAAAVNVSPRSVQYAFRRNL
                     GTTPLEYLRRVRLDRAHRDLKAADPTNDTVTAIAGRWGFSHAGRFSLAYKAAFGTAPS
                     DTLRAYPA"
     gene            834..1313
                     /locus_tag="VGP27_24355"
     CDS             834..1313
                     /locus_tag="VGP27_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HEV7854466.1"
                     /translation="MTERMEVQRTIAAPAADIFAVLCDPHGHVAIDASGMLQDADGEA
                     AKAAGDSFVIHMDRESLNDFPMGKYDVTVEIRDFEMDRLIAWTILGQIRPQIGHVYGY
                     CLEPAEAGGTVVTSFYDWSDIDPEWRKANIFPVIPETALRATLGILDRTVRRGYPRG"
     gene            1364..2092
                     /locus_tag="VGP27_24360"
     CDS             1364..2092
                     /locus_tag="VGP27_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV7854467.1"
                     /translation="MTEPAKIAHPVRLDDLIDVIKTVHAEPLEQLTDAVLAAESLGEI
                     ADHLIGHFVDQARRSGASWTDIGKSMGVSKQAAQKRFVPKVPDFESATLDPNAGFGRF
                     TPRARNVVVVAQNKAQKAGNHEITPNHLLLALFDDPDGLAAKLLAGQGIDGDTVANAV
                     TLPPRADGEMPTLIPFNGPAKKALELTFRQALRLGHNYVGTEHIALALYEDEDGDGPL
                     HRLGVDRNRFESELVTALEQFTKN"
     gene            2128..>2225
                     /locus_tag="VGP27_24365"
     CDS             2128..>2225
                     /locus_tag="VGP27_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HEV7854468.1"
                     /translation="MKVRPFEDVVAEHGATVLRVCRAVVGPLDAEDA"
BASE COUNT          436 a          767 c          656 g          366 t
ORIGIN      
        1 cagaagtgac agctggcgat cgatggcgct gacgtcgcgg tgtttcacta ctccccaatc
       61 accggtaacc cggatgcggc aatcgatcgt aggcgccgaa acgtcgcttc gccggtattg
      121 gatccggcgt gccgacgaca tttccgcgtc ccatgccggc cctgcgacat tccaagtcct
      181 gcagggctct tacgggcgcg acaacactag cgtctactac ttcgaccggc cgatcgccaa
      241 cgcggacccg gatgggctat tgaaagacgg caatcaccgg tgccgacccc gcgggcattt
      301 ctacccggca aagccttcac aagttgttcg gattcgtcaa tatcactcgc gcagaagcgc
      361 ttacgccgga taggcccgca acgtgtcgct cggcgcggtg ccgaacgccg ccttataggc
      421 caggctgaag cggccggcgt ggctgaaccc ccatcgaccc gcgatcgccg tgacggtgtc
      481 attggtcggg tcggcggctt tcagatcgcg atgcgcccga tcgagacgca cgcgccgcag
      541 gtactccaac ggcgtcgtgc ccagattccg gcggaacgcg tactgaaccg accgcggtga
      601 cacgttcacg gccgctgcga tgtcctccag accaatatcg gcatcggcgt tctcctgaat
      661 gaaggcgacc gcattccgca agacgggcgg catcccccgc ccgtttatgt gaccagccac
      721 taccccgcac tcccctgttc ggttggtcaa acgaaacgtt gtggtgcgta tacccgatgc
      781 caacccgccg aaaacaagcg cggcaaagtc ctgccgcctg acaggatcaa cccatgaccg
      841 aacgcatgga ggtccagcgc accatcgctg cgccggccgc cgatatcttc gccgtactct
      901 gcgacccgca cggccacgtc gccatcgacg catccggcat gctgcaggac gccgacggcg
      961 aggccgccaa ggctgccggg gacagcttcg tcatccacat ggatcgcgag tccctcaacg
     1021 actttccgat gggcaaatac gacgtgacag tcgagatccg cgatttcgag atggaccggc
     1081 tcatcgcgtg gacgatcctc ggccagatcc gcccgcagat cggccatgtc tacgggtact
     1141 gcctcgagcc ggccgaagcc ggcgggaccg tcgtcacctc gttttacgac tggtcggaca
     1201 tcgatcccga gtggcgcaag gccaacatct tccccgtcat tcccgaaacc gcgctgcggg
     1261 cgacgctggg catcctcgac cgcaccgtgc ggcgcggcta tccgcgcgga tagcgtcaac
     1321 ggtgggttga cgataaacat aacgtcaacc tagagttgac ggaatgaccg agccggccaa
     1381 gatcgcccac cccgtccgcc tcgatgacct catcgacgtc atcaagacag tgcacgccga
     1441 acctctcgaa caactcaccg acgcggtatt ggcggccgag tccctcgggg agatcgccga
     1501 ccatttgatc ggccacttcg tcgaccaggc ccggcgctcg ggagcgtcat ggaccgatat
     1561 cggcaaatcc atgggcgtca gcaaacaggc cgcgcagaag cgattcgtgc ccaaggtgcc
     1621 cgacttcgaa tccgcgaccc tcgatcccaa cgcggggttc ggtcggttca ctccccgcgc
     1681 gcgcaatgtg gtcgtcgtcg ctcagaacaa ggcgcagaag gccggcaatc acgagatcac
     1741 cccgaaccat ctgctgctcg cgctcttcga cgatccggac gggctcgcgg ccaaacttct
     1801 cgccgggcag ggcatcgatg gcgacaccgt cgccaatgcc gtcacattgc cgccgcgcgc
     1861 ggacggtgag atgccgaccc tgattccgtt caacggacct gcgaaaaaag cgctcgaact
     1921 gaccttccgg caggcacttc gattgggcca caactacgtc gggaccgagc acatcgcgct
     1981 cgcactctac gaagacgaag acggcgacgg cccgctgcac cggcttggcg tcgaccgaaa
     2041 ccgcttcgaa agcgagctcg tcaccgcgct ggagcagttc accaagaact gatctcacac
     2101 acatactcgc cgcggcgtct tcatgatgtg aaggtgcgac cgttcgaaga cgtggtagcc
     2161 gagcacggtg cgaccgtgct gcgggtgtgc cgcgcggtcg tcggccccct cgacgccgag
     2221 gacgc
//
LOCUS       DASYLX010001038         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3030954,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001038 DASYLX010000000
VERSION     DASYLX010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030954"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VGP27_24370"
     CDS             complement(<1..1023)
                     /locus_tag="VGP27_24370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854469.1"
                     /translation="MDFNLTKEQELLRDGLTKFLSTRYALEKSRAAAKTGAGWQPEIW
                     RAFADELGILGATSPEDVGGSGGGPVELMVITEALGHALVIEPYVDTVVVAGGLLHRA
                     GGEQATSLLEKIVAGTAIVALAAGESESGDRWLDVTTTAQREGDDWILNGAKTVATTA
                     PLATHLLITAKSPEGVSLFAVDVESAGSGLSLTGYRTVDDRRAADLVLDGLRVPAGAL
                     LGEAGQAWPSIEQARDEGAAAICSEAVGCMRKVLADTVEYCKQRRQFGVPIGNFQVLA
                     HRMVDMHIELEQAVAAVLLAVLHLEDEPVVRARSVSAAKATVGRAARFIGQQAVQLHG
                     GMGMTEE"
     gene            complement(1024..2256)
                     /locus_tag="VGP27_24375"
     CDS             complement(1024..2256)
                     /locus_tag="VGP27_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854470.1"
                     /translation="MDLQWSEADIAFRDEVRSFLDEKLTPELRTAGRLMTSVYADHDA
                     SMEWQRILHERGWAAPAWPVEHGGCDWSLTQHYIFSRESILAGAPSLSPMGIRMVAHA
                     IIAFGTDAQKDYFLPAILTGEVFFCQGYSEPEAGSDLASLSMSATDDGDDLVCTGSKI
                     WTTHAREANWMFGLVRTSRVEKKQQGITFVLIDMTSPGIQIRPLVMTSGEEVQNQVFF
                     DEVRVPKANVVGKIDDGWTVAKYLLEFERGGGASSPALQVMAGEIATVAAEMPGPEGG
                     RLIDDHAFARKLADARIRTEVLEVLEYRVLAAVSEGENPGTASSMLKVVATELSQTLT
                     EIAMEAAGPRGRAYQPHATRPGGPVTEFEPPPDGYVSGEPWQAVAPLRYFNDRAGSIY
                     AGSNEIQRNILAKAELGL"
     gene            2370..3440
                     /locus_tag="VGP27_24380"
     CDS             2370..3440
                     /locus_tag="VGP27_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854471.1"
                     /translation="MDYQISPDDRAQFKRCRRQWDFASPHRRGLEPVQASESFALATA
                     FRDALAVYYYPGTWDWQHELKQSLVHKAMRRSLEDAGCADELDRATSLIDLYDAWAAT
                     VDDFAPVKIQHDVTALLRDPSDPDNGLLTSDGVAVTYACRVDLLAVDATDEYWVVRHQ
                     IVDEWRAVEEMVLDEEATAACWAWEQDYIGMEIAGTVHNEVRLGEPQVPRVDASAAMG
                     KAEPVAQHEASGGGRSIPQHVRRAVRESRAAADRVEQLTAGMVRHTRIRRSRDEIAAA
                     GSLIATEAIEMTAAPVIYPSPGPHCLACQFMPPCLRMFEGADPRPQLALDFRRRSGAG
                     EHKPRLGQATWGFGRGAAPPDW"
BASE COUNT          611 a         1201 c         1164 g          615 t
ORIGIN      
        1 ttcctcggtc atgcccatcc cgccgtgcag ttggacagcc tgctggccga tgaaccgggc
       61 cgctctgccg accgtcgcct tcgctgccga caccgaccgc gcacgaacga caggctcgtc
      121 ttcgaggtgc agtacggcga gcagaacggc ggcgacggcc tgctccagct cgatgtgcat
      181 atcgaccatg cgatgcgcca gcacctggaa attaccgatc ggcacaccga actgccggcg
      241 ctgtttgcaa tattcgaccg tgtccgccag cacctttcgc atgcatccca cggcttccga
      301 acagatggcg gctgcacctt cgtcgcgcgc ctgctctatc gacggccacg cctgtccggc
      361 ttcgccgagc aacgcgcctg cgggcacgcg cagaccgtcg agcaccagat ccgcggcgcg
      421 ccggtcgtcc acggtccggt atccggtgag cgatagaccg gaacccgcgg attccacgtc
      481 gacggcgaac agcgagacgc cttcagggct cttcgcggtg atcagcagat gggtggccag
      541 cggcgcggtc gtcgctacgg tcttggcgcc gttgaggatc caatcatcac cttcgcgttg
      601 cgccgtggtc gtgacgtcca gccagcgatc gccggattcg gactcaccgg cggccagcgc
      661 gacgatcgcg gtgcccgcca caatcttctc cagcagcgac gtggcctgct cgccaccggc
      721 gcggtgcaga agtccgcccg ccacgaccac cgtgtcgacg tagggctcga ttaccagagc
      781 atgacctagc gcctccgtga tcaccatgag ctctactggc ccgccgccgc ttccgccgac
      841 atcctcgggc gaagtggcac ccaggattcc gagttcgtcg gcgaacgccc gccatatctc
      901 aggctgccat ccggcgccgg tcttggccgc cgcacggctc ttctcgaggg cgtaacgggt
      961 ggaaagaaat ttcgtcaaac cgtcgcggag cagttcctgc tccttggtca ggttgaagtc
     1021 catctacagc cccaattccg ccttggcaag gatgttccgc tgaatctcat tgctgccggc
     1081 atagatcgaa ccggcccgat cgttgaagta gcgcagcggt gcgaccgcct gccacggctc
     1141 gccgctgacg tagccgtccg gtggcggctc gaattcggtg acagggccgc cgggccgggt
     1201 ggcatggggt tggtaggcac gcccgcgtgg tccggcggcc tccatggcta tttcggtcag
     1261 cgtttggctg agctcggtgg ccaccacctt gagcatcgac gacgccgtgc ccggattctc
     1321 gccctcggac accgccgcga gcacccggta ctcgaggacc tcgagcacct ccgtgcgtat
     1381 ccgcgcgtcg gcgagcttgc gcgcgaacgc gtggtcgtcg atgaggcggc ccccctccgg
     1441 gccgggcatc tcggcggcca ccgtcgcgat ctccccggcc atcacctgca gtgcaggcga
     1501 ggacgcgccg ccgccgcgct cgaattcgag taggtacttc gccaccgtcc agccgtcgtc
     1561 gatcttgccg acgacgttgg ccttggggac ccgaacctcg tcgaagaaga cctggttctg
     1621 cacctcctca cccgaggtca tcaccagcgg gcgaatctga atgcccggtg aggtcatatc
     1681 gatcaacacg aaggtgatgc cctgctgctt cttctcgact cgcgaggtgc gcaccaagcc
     1741 gaacatccag ttggcctcgc gggcgtgcgt ggtccagatc ttgcttccgg tgcagaccag
     1801 atcatccccg tcgtccgttg cggacatcga cagcgatgcc aggtccgacc cggcttcggg
     1861 ttcggagtaa ccctgacaga agaacacctc gccggtcagg attgcaggca ggaagtagtc
     1921 cttctgcgcg tcggtgccga aggcgatgat cgcgtgcgcc accatgcgaa ttcccatggg
     1981 ggataacgaa ggagcgcccg ccaggatgga ttccctgctg aagatgtaat gctgggtcag
     2041 gctccagtcg cagccgccgt gttcgacagg ccacgcgggc gcggcccaac ctcgttcgtg
     2101 cagaatccgt tgccactcca tgctcgcatc gtggtcggcg tacacgctgg tcatcagccg
     2161 gcccgcggtg cgcagctccg gtgtcagctt ctcgtcgaga aagctgcgta cctcgtcgcg
     2221 gaaggcaatg tcggcctccg accactgcag gtccatggaa tcccctctgc tcgtaggcac
     2281 attgcaaagc cacttattca gctgaagata atcggagggt ttcgagaccc tcccgaccgg
     2341 gactactctc gggtgcgcaa gggatagctg tggattatca gatcagccca gatgaccgcg
     2401 cacagttcaa acggtgcagg cggcagtggg acttcgcgtc accgcatcgc cgcggtctcg
     2461 aacccgtgca agcgtctgaa tcattcgctc tggcaacggc gttcagggac gcactggctg
     2521 tttactacta ccccggcacc tgggactggc aacacgagtt gaagcagtcg ctggtccaca
     2581 aggcgatgcg gcggtccttg gaggacgcgg gctgcgcgga cgaactcgat cgagcaacgt
     2641 cgttgatcga cctctatgac gcctgggcgg cgacggttga cgacttcgct cctgtcaaga
     2701 tccagcacga cgtgacggcc ctgctacggg acccgtccga tccggacaac gggctgctga
     2761 cctcggacgg tgtggcagtc acctacgcct gccgcgtcga cctgctggcg gtggacgcca
     2821 ccgacgagta ctgggtggtg cggcaccaga tcgtcgacga gtggcgggcc gtcgaggaga
     2881 tggtgctcga cgaggaggcc accgccgcct gctgggcgtg ggagcaggac tacatcggca
     2941 tggagatcgc aggcaccgtg cacaacgagg ttcgtctggg cgaaccgcaa gtccctcgcg
     3001 tcgacgcgtc cgcggcgatg ggcaaagccg agccggtggc acagcacgaa gccagcggcg
     3061 gcggtcggtc gataccgcag cacgttcgga gggcggtccg cgaatcgcga gcggcagccg
     3121 atcgcgtcga gcagctgacg gcgggaatgg tgcgccacac acggatccgg cgcagccgcg
     3181 acgagatcgc cgctgcaggc agcctcatcg cgaccgaagc catcgagatg accgccgcgc
     3241 cggtcatcta tccgagcccc ggtccgcatt gtctcgcatg ccagttcatg ccaccgtgtc
     3301 tgcgcatgtt cgaaggcgcc gacccgcgac cccagctggc actcgacttc cgccgtcgct
     3361 cgggtgcagg cgagcacaag ccacgacttg gccaggccac ctggggtttc ggtaggggag
     3421 cggcgccgcc ggattggtga gctgggcacg cgatgcgcag gcataatcgg cacggtggag
     3481 cggcgtcggg ataacagatc gaagcagcag ccgatggacg tgctgcggga gctgcccgca
     3541 ttggttgtgc tcgagcggtt tccggtgcca gtgctggcca ccggggagga c
//
LOCUS       DASYLX010001039         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3907714,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001039 DASYLX010000000
VERSION     DASYLX010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907714"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VGP27_24385"
     CDS             <1..688
                     /locus_tag="VGP27_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7854472.1"
                     /translation="AMPEQLGRVYLGLTDEDAPGAIPDVPEPTSEEIGFLLDIVNTAL
                     DVKLGRDDVIGAYAGLRPLIDTKEGKTADVSRDHAVVESPSGVLSVIGGKLTEYRYMA
                     EDVLDKAVALQGLSAGRCRTRNLPLVGAPSNPVATLRVPTGLPSSLVARFGAEAPNVI
                     AKATCDRPTDPVADGIDVIRAEFEYAVTHEGALTVDDIVDRRTRIGLVDSDRDSVLTV
                     AEELLARGAR"
     gene            complement(730..1812)
                     /locus_tag="VGP27_24390"
     CDS             complement(730..1812)
                     /locus_tag="VGP27_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7854473.1"
                     /translation="MPLDHYLSLGRSGLRVSPFALGAMTFGEDAGGAGSSVEESEKIL
                     ATYLDRGGNFVDTANFYTNGHSEKILGDWFAAHPGQRGRVVLASKFFANMYPGNPNGG
                     GAGRSSIRTQLHETLRRLQTDYLDMYWLHNWDRHTPIEETLRALDDLVREGKIRYIGF
                     SNTPGWVTAQAQTTAVLRGWTPLIALQVEYSLLARTVEGELAPLALDQGMALVPWSPL
                     KNGFLSGKYTRGAEVSDSVRTAYVGGPSDDDFTVIDAVAAIAAELEISSAAVALAWLR
                     AREGTVVPIVGARKLSHLEDNLAGIDVVLSSEHLSVLDDVSAPTLNYPAEVNGPTRAM
                     LAFAGTTVDGESSTVYPPLLQSDIRY"
     gene            1930..2838
                     /locus_tag="VGP27_24395"
     CDS             1930..2838
                     /locus_tag="VGP27_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7854474.1"
                     /translation="MQSAADSLRDIRARIAAHARPDLLTPIDGLLMSKVMSASAGQEY
                     SLTEPLVVVMAQGGKRLLLGDDVHEYRAGECLVVTASLPVTGYYIDTTAGSPSLAVAL
                     VLRPAVVAELVLQTPAARWTRADNSAMSTGKASPELLDAVVRLLRLIDAPADASVLAP
                     MIEREIIWRLLTGPQGGTIRQIGLPDSDVSLVSRAIDWIRENYAEPMTIDDLARLCGM
                     STSAFHRHFRGVTAVSPLQFQKRIRLQEARSLLVARPGDVAGVGHRVGYDSPSQFNRE
                     YRRLFGVPPGQDAAQLRADVVLERGL"
     gene            complement(2833..3222)
                     /locus_tag="VGP27_24400"
     CDS             complement(2833..3222)
                     /locus_tag="VGP27_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
                     /protein_id="HEV7854475.1"
                     /translation="MRISGTTVRRGIAGVIATGALAGLSGAIAMPTASADADCTVSGL
                     SNAIGAVGAQSGQWFARHPDANKAVTEAGGQGGGAEAGIRNYFVAHPTEWAELQGIAS
                     PLRTLRQQCGSDVAPADIARLYDAMAS"
     gene            complement(3352..>4099)
                     /locus_tag="VGP27_24405"
     CDS             complement(3352..>4099)
                     /locus_tag="VGP27_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV7854476.1"
                     /translation="GDTDLHALLPDSPRRAYDVHPLVEALLDSDAPFEEFQAKWAPSM
                     VIGLGRLSGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLVVVVDVPGYL
                     PGVDQEWGGVVRRGAKLLHAFGEATVPRVTLVTRKIYGGAYIAMNSRSLNATKVFAWP
                     DAEVAVMGAKAAVGILHKRALAAAPDDEREAMHEELAAEHERIAGGVDSAIDIGVVDE
                     KIDPAHTRSRLTQALAEAPARRGRHKNIPL"
BASE COUNT          674 a         1385 c         1356 g          684 t
ORIGIN      
        1 cgcgatgccg gagcagctgg gccgggtgta tctgggactg acggatgagg acgcgccggg
       61 cgcgataccc gatgtccctg agccgacttc ggaggagatc ggcttcctgc tggacatcgt
      121 caacaccgca ttggacgtca agctcggccg agatgacgtc atcggtgcct acgcggggct
      181 gcggccgctg atcgacacga aagaaggaaa gacggccgac gtgtcgcgcg accacgccgt
      241 ggtcgagtcg ccatcggggg tgctcagcgt gatcggcggc aaactgaccg aataccggta
      301 catggccgag gatgttctcg acaaggccgt cgcattgcag ggtctgtcgg caggccgctg
      361 ccggacgcga aacctgccgc tcgtgggtgc gccgtcgaat ccggttgcga cgcttcgcgt
      421 tcccactggc ctaccgtcgt cgctggtcgc gcgattcggc gccgaggcgc ccaatgtcat
      481 cgccaaggca acctgcgacc ggccgaccga tccggtggct gacggcatcg acgtgatccg
      541 cgcggagttc gagtacgcgg tcacgcacga gggcgcgctg accgtcgacg acatcgtcga
      601 ccggcgcacc cgcatcgggc tggtcgactc cgaccgggac tcggtgttga ctgtggcgga
      661 ggaactgctc gcacgaggtg cgcgatgagt ctggcccacg gacgcccaac ccagggttcc
      721 aggcctgact cagtaccgga tgtcactctg caacaacggc gggtacaccg tcgaggactc
      781 cccgtcgacc gtggtgcccg cgaacgcgag catcgcccgc gtcgggccgt tgacctcggc
      841 agggtaattc agtgtcggcg ccgacacgtc gtcaaggacg ctcagatgtt ccgagctcag
      901 caccacgtcg atcccggcga gattgtcctc gaggtgtgac agcttgcggg cgccgacgat
      961 gggcaccacg gtaccctcac gcgcccgcag ccaggcgagt gctactgcgg cggagctgat
     1021 ttcgagttca gcggcgatgg ctgcgaccgc atcgatcacc gtgaagtcgt cgtcgctcgg
     1081 tccgcccacg taggccgtgc gcacggaatc gctgacctcg gcgccgcggg tgtatttgcc
     1141 cgaaaggaag ccgttcttca aggggctcca cggcacgagc gccatcccct gatcgagcgc
     1201 aaggggtgcg agttcgccct cgaccgtgcg ggcgagcagc gagtactcaa cctgcagcgc
     1261 gatcagcggg gtccagccgc gcagcacggc tgtcgtctgc gcctgcgcgg tgacccaacc
     1321 gggggtgttc gagaaaccga tgtagcggat cttgccttcc cggacgaggt cgtccagtgc
     1381 gcgcagagtc tcctcgatcg gcgtgtggcg gtcccagttg tgcagccagt acatgtccag
     1441 atagtcggtc tgcagcctgc gcaacgtttc atgcagttgc gtcctgatcg acgagcggcc
     1501 cgcgccaccg ccgttcggat tacccgggta catgttcgcg aagaacttcg acgccagcac
     1561 cacgcggccg cgttgaccgg gatgggcggc gaaccaatcg ccgaggatct tctccgaatg
     1621 accgttggtg tagaagttcg cggtgtcgac gaagttgccg ccacgatcca agtaggtcgc
     1681 gaggatcttc tcggactcct cgacgctgga cccggcccca cccgcgtctt cgccgaacgt
     1741 catcgcgccc agagcgaacg ggctgacgcg cagcccggac cggccaagtg agagatagtg
     1801 atcgagtggc atgaaactgc tccttgctgt gtcggatgta tctactatcc aaccgaacat
     1861 gcgaggtcaa cggtagtttg atccgagcaa cctcttgcac aatccactca ttcttgctgt
     1921 gatcggagta tgcagagtgc agcggactcg ctccgcgaca tcagggcccg catcgcggcg
     1981 cacgcccggc ccgatctgct gacgccgatc gacggtctgc tgatgtcgaa ggtgatgagc
     2041 gcgtccgcag ggcaggaata ctcgctgacc gagccgctcg tggtcgtgat ggcccaaggg
     2101 ggcaagcgcc tcctcctcgg cgacgacgtg catgagtacc gcgcgggcga gtgtctcgtc
     2161 gtgaccgcca gcctgccggt caccggctac tacatcgaca ccaccgccgg ctcaccgtcg
     2221 ctggccgttg cgctcgtgct ccgcccagcc gttgtggccg aactcgtgct gcagacgcct
     2281 gcggcccggt ggacccgcgc ggacaactcg gcgatgtcga cgggcaaagc cagtcccgaa
     2341 ctgctcgatg cggtggtccg cttgctgcgc ctgatcgatg cccctgccga cgcatcggtc
     2401 ctcgcgccga tgatcgagcg ggagatcatc tggcgcctgc tcaccggccc gcaaggcggc
     2461 accatccgcc agatcggtct ccccgacagc gacgtgtcgc ttgtcagccg cgcgatcgac
     2521 tggatccgag agaactacgc cgagccgatg acgatcgacg acctcgcgcg gctgtgcggc
     2581 atgagcacat cggcgttcca ccgccacttt cgcggagtaa cggccgtgag cccgttacag
     2641 tttcagaagc gcatccggct gcaggaagcc cgctcgttgt tggtcgcgcg ccccggtgac
     2701 gttgcgggcg tcggtcaccg cgtcggctat gacagcccct cacagttcaa tcgcgaatac
     2761 cggcggttgt tcggcgtccc gccggggcag gacgccgccc aactacgtgc cgatgttgtg
     2821 ctcgaacgag ggttatgagg ccatcgcgtc gtagagccgc gcgatgtccg ccggcgccac
     2881 gtccgatccg cactgctggc gcagcgtgcg caacggcgat gcgattccct gcagttccgc
     2941 ccactcggtc gggtgggcga cgaagtagtt ccggatcccg gcctcggctc cgccgccctg
     3001 gccgccggct tcggtgactg ccttgttcgc atccggatgg cgggcgaacc actggccgct
     3061 ttgcgcgccg accgcgccga tcgcgttgct cagcccggac acggtgcagt cggcgtcggc
     3121 ggatgccgtc ggcatcgcga tggcgccgct cagtcccgca agcgctccgg tcgcgatgac
     3181 tccggcaatg ccgcggcgca cggtggttcc agaaatacgc atacctcaac tcctctgtcc
     3241 gggccctgca tggttccccc aactcaatgg gccgccaccc acattacgga agcgcagggt
     3301 ttgccggggt cacctcgaac agttcggcaa aaccacgaat gtgaaatcga cctacagcgg
     3361 aatgttcttg tgccgcccgc ggcgtgccgg cgcctcggcg agtgcctgcg tcagcctgct
     3421 gcgggtgtga gccggatcga tcttctcgtc gaccacgccg atgtcgatgg cggaatccac
     3481 accgcccgcg atccgctcgt gctcggcggc cagctcttca tgcattgcct cacgctcgtc
     3541 gtcgggcgca gcggccagcg cgcgcttgtg caggatgccg acggcggcct tggcgcccat
     3601 cacggcgacc tcggcgtccg gccaagcgaa caccttcgtc gcgttgagcg agcgtgagtt
     3661 catcgcgatg taggcgccgc cgtagatctt ccgggtgacc agcgtgacgc gggggacggt
     3721 ggcctcaccg aacgcatgca acagcttcgc gccgcggcga accacgccgc cccactcctg
     3781 gtcgacaccg ggcaggtaac cgggcacgtc gaccacaacg accagaggga tgccgaacgc
     3841 atcgcacagc cgcacgaaac gtgccgcctt ctcggcgctt tcggagttca ggcagccgcc
     3901 cagccgcagc gggttgttgg cgagcacgcc gacggttcgc ccggacagcc ggccgagccc
     3961 gatcaccatc gacggggccc acttggcctg gaactcctcg aagggggcat cggagtcgag
     4021 cagcgcttcg acgagcgggt gcacgtcgta ggcacggcga ggcgaatccg gcagcagggc
     4081 gtgcaggtcg gtgtcgccc
//
LOCUS       DASYLX010001040         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2482727,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001040 DASYLX010000000
VERSION     DASYLX010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482727"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2291
                     /locus_tag="VGP27_24410"
     CDS             <1..2291
                     /locus_tag="VGP27_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV7854477.1"
                     /translation="LSATVSNAEEFGGWIQTVRGDTTVVVEEHRPVPLWQHLMVGKRL
                     FDLFDYRSGGTKKNGRELLVNPELLRHIAHRREADRLTDWHSGGRGGHRSRPRIYRGP
                     SRPDVIELLEDEGLLPAITFIFSRAGCDAAVKQCLRSSLRLTTEAERVRIAEVVDRRC
                     AELSESDLVVLGYHEWRDGLLRGLAAHHAGMLPVFRHTVEELFTEGLVRAVFATETLA
                     LGINMPARTVVLERLVKFDGEQHVQLTPGEYTQLTGRAGRRGIDVEGHAVVLWTPDVD
                     PAEVAGLASTRTFPLRSSFAPTYNMTINLVNKMGPAEARKLLERSFAQYQADRSVVGL
                     VRGIERGERMLDEIAGELRGRADERSREEQKTEPPILEYARMRATISQREKAQSRASR
                     LHRRRATNDALSSLRRGDIVNIVNGRRAGLAVVLEAARDDDDPRPLVLTENRWAGRIS
                     AADYSGASAKVGSMTLPKRVEHRQPRVRRDLASALRSATDGMAIPKGRRSGPPRDHDV
                     DPELAGLREQLQRHPGHRLSDREEKARQAERYLRIERDNEQIRQKVAAATNSLARTFD
                     RIVELLTERGFIRETGGDLRVTDDGKLLARIYNESDLLVAECLRGGVWEGLTPAELAG
                     VLSAVLFESRGDSPATPQAADIPTGKLRRALADTRRFAAELRADEQRHRISQSREPDE
                     GFVAAIYRWATTGDLAAALDASDVGCTGSPLSAGDFVRWCRQVVDLADQVRNAAPTPQ
                     LRAAAKRAIAEVRRGVVAVDAG"
     gene            2332..3063
                     /locus_tag="VGP27_24415"
     CDS             2332..3063
                     /locus_tag="VGP27_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7854478.1"
                     /translation="MSGPQGPDQTQPWPGQDQPSSDPSHQPAWQQPAPGQDQAAWQQQ
                     PPAYSPQQYPPYQQPGQQPYQPPQQYPPTEQFGQQPTHDYSQQPYPQYGQQPGQYGQQ
                     PGQYGQQPGQPGQYGQYPQYGSTETQEGSKRSLAVVGGVIGLLAALVVAVVAVLGFWK
                     PGFFVTTKLDVSAAQSGVQQILTDESNGYGAKNVKDVKCNNGQSPTVKKGETFDCEVS
                     IDGTKRQVTVTFQDNSGTYEVGRPK"
     gene            complement(3071..>3634)
                     /locus_tag="VGP27_24420"
     CDS             complement(3071..>3634)
                     /locus_tag="VGP27_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEV7854479.1"
                     /translation="GRERKDSVVVVSGDRDLLQLVRDEPVSVRVLYLGGGLAKATKWG
                     PEEVAEKYGVPVNRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLEDIL
                     AAAHNPRSKMSKAYRKKLLAATEYIEAAGPVVKVATDAKVNFSTSSDTLPLSAEHPRK
                     VVKLAEQYGVTSSIGRLQKALDALPGD"
BASE COUNT          626 a         1227 c         1247 g          534 t
ORIGIN      
        1 gcctgtcggc gacggtcagc aacgccgagg agttcggtgg ctggatccag acggtgcgcg
       61 gcgacacgac ggtcgtcgtc gaagaacacc gtcccgttcc gctttggcag cacctcatgg
      121 tcggcaagcg gctgttcgac ctcttcgact accgctccgg cggcacgaag aagaacggtc
      181 gcgagctgtt ggtgaacccg gaattgttgc ggcacatcgc gcatcgacgc gaagccgacc
      241 ggctgaccga ttggcattcg ggcggccgag gtggccaccg aagccgaccg cgaatttacc
      301 gggggccgtc acggcccgac gtcatcgaac tgcttgagga cgaaggcctg ctgcccgcca
      361 tcacgttcat cttctcccgg gccggctgtg atgcggcggt caagcagtgc ctgcgttcgt
      421 cgctgaggct caccaccgag gctgaacggg tccgcatcgc cgaggtcgtc gaccgccggt
      481 gcgccgagct gtccgaatcc gatctcgtcg tgctgggcta ccacgaatgg cgcgacggcc
      541 tgttgcgcgg gctggccgca caccacgccg ggatgctgcc tgtattccgc cataccgtgg
      601 aagagctgtt cacggagggt ttggtcaggg ccgtattcgc caccgagaca ttggctttgg
      661 gtatcaacat gcccgctcgc acggtcgtcc tggagcggtt ggtcaagttc gacggagagc
      721 aacacgtcca gctgacgccg ggggagtaca cgcagttgac cggccgtgcg ggccgtcgcg
      781 gcatcgatgt cgaaggtcac gccgttgtgc tgtggacccc ggacgtggac cccgcggaag
      841 tggccggcct cgcatcgacc cgcaccttcc cgctgcgcag ttcgttcgcg ccgacctaca
      901 acatgaccat caacctggtg aacaagatgg gaccggcgga agcgcgcaag ctgctggaaa
      961 ggtccttcgc gcagtaccag gccgaccgtt cggtggtcgg gctggtgcgc ggcatcgagc
     1021 gcggcgagcg gatgctcgac gagatcgccg gggagctgag ggggcgcgcc gatgagcgct
     1081 cgcgcgaaga gcagaagacc gagcccccga ttctggagta tgcgcggatg cgcgcaacga
     1141 tcagccaacg cgagaaggct cagtcacggg cgtcgcggct gcatcgcaga agggccacca
     1201 acgatgcgct gtcgtcgttg cggcgcggcg acatcgtcaa catcgtcaac gggcggcggg
     1261 ccgggctggc ggtcgtgctc gaggcggccc gcgacgacga cgatccacgc ccgctggtgc
     1321 tgaccgaaaa ccgttgggcg ggacgcattt ccgcggcgga ctactcgggc gcatcggcaa
     1381 aggtgggttc gatgacgctg cccaaacggg tcgagcaccg ccagccgcgg gtccgtcgcg
     1441 atctggcgtc ggcgctgcgg tcggccaccg acgggatggc gatacccaag ggcaggcgaa
     1501 gcggcccacc gcgcgaccac gacgtcgacc cggagctggc gggtctacgc gagcagctgc
     1561 agcgccaccc cggccatcgg ctgtcggacc gcgaggagaa ggccagacag gccgagcgtt
     1621 atctgcgcat cgaacgcgac aacgagcaga tcaggcagaa ggtggccgcg gcgaccaact
     1681 cgctggcccg gacgttcgac cgcatcgtcg agctgctcac cgagcgcggc ttcatccgcg
     1741 aaaccggcgg tgacctcagg gtgaccgacg acggcaagct gctggcccgc atctacaacg
     1801 agagcgatct gctggtagcc gagtgcctcc ggggcggcgt ctgggagggt ttgacgcccg
     1861 ccgagctggc cggtgtgctg tcagcggtgc tgttcgagtc gcggggtgac tcgccggcga
     1921 cgccgcaggc cgccgacatc ccgaccggaa agctgcgtcg tgcactcgcc gacacccgcc
     1981 ggttcgccgc cgaactgcgc gccgacgagc agcggcaccg gatctcgcag agccgcgaac
     2041 ccgacgaggg cttcgtcgcc gccatctacc gatgggccac caccggcgac ctggccgccg
     2101 cgctcgacgc atccgacgtc ggctgcaccg gctcgccgct ctcggcgggc gacttcgtgc
     2161 gctggtgccg gcaggtcgtt gatcttgccg atcaagtgcg caacgccgcg ccgacaccgc
     2221 aactccgagc cgccgcgaaa cgcgccatcg ccgaggttcg acgcggcgtc gtcgctgttg
     2281 atgcagggta aggtgtgggc aattggcgac caccaaggac gagggagaga gatgagcgga
     2341 ccgcagggac ctgaccagac gcagccgtgg ccgggtcagg accagccgtc gagcgacccg
     2401 tcccaccaac ccgcgtggca gcagccggcg cccggtcagg accaggcggc atggcagcag
     2461 caaccgcccg cctactcgcc gcagcagtac ccgccctatc agcagccggg ccagcagccg
     2521 tatcaaccac cgcagcagta cccgccgacc gagcagttcg gccaacagcc gactcacgat
     2581 tacagccagc agccctatcc tcagtacggc cagcagcccg gtcagtacgg acagcagccg
     2641 ggccagtacg gtcagcagcc gggccagccg ggccagtacg gtcaataccc ccagtacggc
     2701 tcgaccgaga cgcaggaggg ttccaagcgt tcactggccg tcgtcggcgg tgtgatcggc
     2761 ctgctcgccg cgctcgtcgt cgccgtcgtg gcggtgctgg gcttctggaa gcccggcttc
     2821 ttcgtcacca ccaagctgga cgtcagcgcc gcgcagtcgg gtgtccagca gatcctgacc
     2881 gacgagtcca acggctacgg cgccaagaac gtcaaggacg tcaagtgcaa caacggtcag
     2941 agcccgacgg tgaagaaggg tgagacgttc gactgcgagg tcagcatcga cggcaccaag
     3001 cgtcaggtca ccgtcacctt tcaggacaac agcggcacct atgaggtcgg tcggccgaag
     3061 tagctgaatg ctagtcgccg ggcaacgcgt caagtgcctt ctgcagtcgg ccaattgacg
     3121 atgtgacgcc atactgctca gcgagcttga ccaccttgcg cgggtgctcg gcggacagcg
     3181 gcagcgtgtc ggacgacgtc gagaaattca cctttgcgtc ggtggcgacc ttgaccaccg
     3241 gcccggcagc ctcgatgtat tcggtggcgg ccagcagctt cttccggtag gccttcgaca
     3301 tcttcgatcg cggattgtgc gcggcggcga ggatgtcctc cagcgagccg tgctgggcga
     3361 gcagggtggc cgccgtcttc tcgcccacgc cgggaacacc gggcagtccg tcggacggat
     3421 cgccccgcag cagcgccagt tcggcgtagg ccggtccggc gcggttgacc gggacgccgt
     3481 acttctcggc gacctcttcg ggtccccact tcgtcgcctt ggccaggccg ccgccgaggt
     3541 agagcacgcg gaccgacacc ggctcgtcgc gaaccagctg aagcaggtcg cggtcaccgc
     3601 tgaccacgac gacggagtcc ttgcgctccc ggcc
//
LOCUS       DASYLX010001041         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1825103,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001041 DASYLX010000000
VERSION     DASYLX010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825103"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /gene="cobI"
                     /locus_tag="VGP27_24425"
     CDS             complement(<1..522)
                     /gene="cobI"
                     /locus_tag="VGP27_24425"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEV7854480.1"
                     /translation="MTDRGTLWGVGLGPGDPELVTVKAARVIAQSDVVAYHSARHGRS
                     IARGIAQPYLRQGQVEEHLVYPVTTETTDHPGGYAGAMEDFYREAAGRIAAHLDAGRD
                     VALLAEGDPLFYSSYMHMHTRLTERFDAVIVPGVTSVSAASAATGTPLVQGDEVLTII
                     PGTLPVDELKRRLA"
     gene            complement(519..1145)
                     /locus_tag="VGP27_24430"
     CDS             complement(519..1145)
                     /locus_tag="VGP27_24430"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEV7854481.1"
                     /translation="MLDYIRDAAEIYRESFATIRAEADLSRFPDDVARVVVRLIHTCG
                     QLDVVEHVAHSEDVVANTHAALAAGAPVLCDSSMVAAGITRSRLPADNEVVSLVADGA
                     AADLAARLGTTRSAAAVELWADRFGGAVLAIGNAPTALFRLLELVDDGAPTPAAVLGG
                     PVGFVGSAQSKQELIDRPRGMSYLVVTGRRGGSAMAAAAVNAIASSTE"
     gene            complement(1155..>1908)
                     /locus_tag="VGP27_24435"
     CDS             complement(1155..>1908)
                     /locus_tag="VGP27_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HEV7854482.1"
                     /translation="AEPALAALPGRFWFGIDDGRGDVSGLAADAGAHVCGDSVAVLLA
                     GRDTGIRVAASEVVPTLVSVARRFVEFREDAWRVAELADPAALIEGLPRSAEPGATWP
                     PVSRPPVGWISQDDGRTALGAAAPLGVLQARVAEYLAAIDAPMVITPWRSVLVCDLDE
                     GVADAALRVLAPMGLVFDDNSPWLDVSACTGSPGCEHSAADVRADAARAVGDSSTAHR
                     HFVGCERACGSPPSGDVLIATGDGYVLRTASP"
BASE COUNT          279 a          679 c          690 g          260 t
ORIGIN      
        1 ggcgagcctg cgcttgagct cgtcgacggg cagggtgccg ggaatgatcg tcaacacctc
       61 gtcgccctgc accaggggtg tgcccgttgc ggccgatgcc gcgctgaccg acgtcactcc
      121 cggcacgatc accgcgtcga agcgctcagt gagccgggtg tgcatgtgca tgtaggagct
      181 gtagaacagc ggatcgccct cggcgagtag tgcgacgtcg cggcctgcgt ccagatgcgc
      241 ggcgatccgc ccggcggcct cgcggtagaa gtcctccatc gcgccggcgt aaccgccggg
      301 atggtcggtc gtctcggtgg tcaccggata caccaggtgt tcctcgacct ggccctgccg
      361 cagatacggt tgcgcgatgc cgcgggcgat gctgcggcca tgcctcgcgc tgtgatacgc
      421 gacgacgtcg gactgcgcga tgacccgggc ggccttgacc gtcaccagct cggggtcgcc
      481 agggccaagt ccgacgcccc acagcgttcc tcggtcggtc attcggtgct actcgcgatg
      541 gcgttgacgg cggcggcggc catcgcgctt ccgccgcggc gtccggtgac caccagatag
      601 gacatgccgc ggggccggtc gatgagctcc tgcttggact gcgccgagcc gacgaacccg
      661 acgggacctc cgagcacggc cgccggggtc ggcgcgccgt cgtccacgag ttccagcagc
      721 cggaacagcg cggtcggcgc attgccgatc gcaagcaccg caccgccgaa ccggtcggcc
      781 cacagctcca ccgcggcggc cgagcgggtg gtgccgagcc gggccgcaag gtcggcggcc
      841 gcgccatcgg ccaccagcga caccacctcg ttgtcggcag gcagccggga ccgtgtgatt
      901 cccgccgcca ccatcgacga gtcgcatagc acgggtgcgc ctgcggcgag cgcggcgtgc
      961 gtgttggcga cgacgtcctc gctgtgggcg acgtgctcga cgacgtcgag ctggccgcac
     1021 gtgtggatca agcgcacgac cacccgggcg acgtcgtccg ggaaccgcga gagatcggct
     1081 tcggcgcgga tcgtcgcgaa cgactcccgg tagatctccg ccgcgtcgcg gatgtagtcg
     1141 agcacccgct caccctacgg ggaggctgtg cgcagcacgt atccgtctcc agtggcgatc
     1201 agcacgtcgc cggatggtgg gctgccgcag gcccgttcgc acccgacgaa gtgccggtgt
     1261 gccgtgctgg agtcgccgac cgcgcgggcc gcgtccgccc gcacgtcggc cgcggagtgt
     1321 tcgcagccgg ggctgccggt gcaggcgctc acgtcgagcc agggtgagtt gtcgtcgaac
     1381 accagaccca tcggcgcgag cacccgcagg gcggcgtccg cgaccccttc gtcgaggtcg
     1441 cagacgagca ccgagcgcca cggtgtgatg accatcgggg cgtcgatggc tgccaggtac
     1501 tcggcgaccc tcgcctgcag cacgccgagt ggcgccgccg cgcccaacgc ggttcggccg
     1561 tcgtcctggc tgatccagcc gaccggcgga cggctgacgg gcggccaggt cgcccctggc
     1621 tcagcggaac ggggtaagcc ctcgatcagc gctgcaggat ccgcgagttc cgcaacccgc
     1681 caggcgtctt cgcggaattc gacgaagcgc cgcgcgacgg atacaagagt cggcaccacc
     1741 tcggaggctg ccacgcgaat tccggtgtcg cggccggcca gcaggacggc gaccgaatcg
     1801 ccgcaaacat gggcgccggc gtcggcggcc agcccggaaa cgtcgccgcg gccgtcgtcg
     1861 atgccgaacc agaaccggcc gggcagggcg gcgagggccg gctcggca
//
LOCUS       DASYLX010001042         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_729441,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001042 DASYLX010000000
VERSION     DASYLX010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729441"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..630
                     /gene="rbfA"
                     /locus_tag="VGP27_24440"
     CDS             136..630
                     /gene="rbfA"
                     /locus_tag="VGP27_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV7854483.1"
                     /translation="MVDVGRARRLSKRIATVVATAIEFEIKDPPLAFVTVTDTKVTGD
                     LHDATVYYTVRGATLQDEPDYAAAAAALERAKGTLRTKVGAATGVRFTPTLAFVLDKV
                     PETSAHMEELLARARAADEDLARVRQGAKHAGDEDPYRQDRVEGIDGEPENELDAEDT
                     VDRD"
     gene            617..>1509
                     /locus_tag="VGP27_24445"
     CDS             617..>1509
                     /locus_tag="VGP27_24445"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV7854484.1"
                     /translation="MTAIDPKTDADLVGQRVDANGAAELLDAAASVSVVCHVFPDADT
                     VGAGLALALVLDHAGKDVEVSFAAPDRLPESLQSLPGGHLLIPPNDMRRDADLVVTVD
                     IPSVNRLGALRELAEPGREVLVIDHHASNQLFGSANFVEPSADSTTMLVADLLDAWNK
                     PIDAGVAHCLYAGLTTDTGSFRWATARAHRLAARLVELGVDNAGISRTLLDTHPFAWL
                     PMLSRVLSSAKLLPDAAAGAGLVYAIVGHQEWVDARPEEVESIVDIVRTTEQAEVAAV
                     FKEIEPGHWSVSMRAKSIDLAA"
BASE COUNT          261 a          497 c          516 g          235 t
ORIGIN      
        1 tcactgcgcc gggagaagga cgacgtcacc gaggtgcgcg acggctacga gtgcggtttg
       61 acgctgacct attccgacat caaggaaggc gacgtcatcg aaacgtacga gctcgtcgaa
      121 aaggagcgca catagatggt cgatgtgggt cgggcccgtc ggctctccaa gcgcatcgcc
      181 accgtcgtcg ccaccgcgat cgagttcgag atcaaggatc ccccgttggc ctttgtgacc
      241 gtcacggaca cgaaggtgac gggagacctg cacgacgcca ccgtgtacta cacggtccgc
      301 ggcgcgacgt tgcaggacga accggactac gcggcggccg cggcggcgct ggagcgggcc
      361 aaaggcacgt tgcgcaccaa ggtcggcgcg gccaccggtg tccggttcac cccgacgctg
      421 gccttcgtgc tggacaaggt gcccgagacc tccgcgcaca tggaggagct gctggcccgc
      481 gcccgcgccg ccgatgagga tttggcgaga gttcggcagg gcgccaagca tgcgggcgac
      541 gaggacccgt atcgtcagga tcgggtggag ggcatcgacg gggagccgga gaacgagcta
      601 gacgctgagg acaccgttga ccgcgattga tccgaagacc gacgcagact tggtgggtca
      661 gcgcgtcgac gccaatggcg cggccgaact cctcgatgcg gcggcctcgg tcagcgttgt
      721 ctgccacgtc tttcccgacg ccgacacggt cggcgcagga ttggcgttgg cgctggtgct
      781 cgatcatgcg ggcaaggacg tcgaagtcag cttcgcggca ccagacaggc tgcccgaatc
      841 ccttcagtcg ctgcccggcg gtcatctgct catccctccc aacgacatgc gccgcgacgc
      901 cgacctggtg gtcaccgtcg acattccgag cgtgaaccgg ctcggcgcgc tacgcgagct
      961 cgcggagccc ggccgcgagg tgctggtgat cgaccaccac gcgtccaatc agctgttcgg
     1021 cagcgccaat ttcgtcgagc cgtccgcgga ctccaccacg atgctggtcg ccgacctgct
     1081 cgacgcgtgg aacaagccga tcgacgccgg ggttgcgcac tgtctgtacg ccgggctgac
     1141 caccgatacc gggtcgttcc gctgggccac tgcacgagca caccggctgg ccgcccggct
     1201 ggtggagttg ggcgtcgaca acgcaggtat cagccgcacg cttctggaca cccatccgtt
     1261 cgcgtggctg ccgatgctgt cgcgcgtgct gtcgtccgca aaactgctgc ccgacgccgc
     1321 cgcaggcgca ggcctcgtgt acgccattgt cggacaccag gagtgggtcg atgcgcggcc
     1381 cgaggaagtc gaaagcatcg tcgacatcgt gcgcaccact gagcaggcgg aggtcgccgc
     1441 ggtcttcaag gagatcgaac cggggcactg gtcggtgtcg atgcgcgcca agtcgatcga
     1501 tctcgccgc
//
LOCUS       DASYLX010001043         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_729705,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001043 DASYLX010000000
VERSION     DASYLX010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729705"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VGP27_24450"
     CDS             <1..652
                     /locus_tag="VGP27_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7854485.1"
                     /translation="PFVTFFSKIGVEGAGTIMNIVVLTAAFSSLNAGLYSTGRILRSM
                     AMNGSAPKFTSLMNKRGVPYGGICLTALIGLFGVLLNGIVPSEAFEIVLNMAALGIIA
                     SWGTIVICQLQLYRWSKKGILERPSFRLWGTPWTGYLTLLFLLAVLVLMALDPPIGTW
                     TVASLIVIIPALVIGWFAVRSRVLAVAQERQGFTGSFPVVANRPPPHEKDRRKKDD"
     gene            complement(872..1867)
                     /locus_tag="VGP27_24455"
     CDS             complement(872..1867)
                     /locus_tag="VGP27_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854486.1"
                     /translation="MAGTVSTPNGRVREALSAAQHMTRDDDSAYVAGSPAGVRRLLPE
                     GRRMRQQVAEQLRSMGMDTDALERELGAARARGKDRLIKDKQAAVSQSGARAESLRHL
                     LDGTVAAFDGLVATTGDTPTQYKTLDSPVQIWATDGINVESTTIQPYNSQAKVRMEAS
                     YYTSFFDAYLAGDEQLLHFQYLWGNPRQDASAVVNINAIVALNGFCSAHSDGGITGGG
                     AARLDLEPTLDLIQTWTQPISSVPPQPGQSYRVARVSADSSGLFSDDEDTSAVVFRGT
                     SVGYDQAIVPPGQYLIIDVALRLYSETVDGKVDADFATGLFNVLSPFVQLAIAFV"
     gene            2014..>2531
                     /locus_tag="VGP27_24460"
     CDS             2014..>2531
                     /locus_tag="VGP27_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEV7854487.1"
                     /translation="MAADIVPIRLGLTKGDLYTLWAPRWRDAGDEWEALLGKDDDLYG
                     FESVADLAAFVRTNTDNDLSDHPAWEATTEANAHAFDPPEERQFDLIGVQELLAEKPT
                     EDSVETLHRTLAIVSSVGSVCELSAVTKFFNGNPVLGSLGLGVDTFSGRSGRKRWSEI
                     GAVIGRSWDNVV"
BASE COUNT          431 a          837 c          790 g          473 t
ORIGIN      
        1 cccgttcgtc acgttcttct cgaagatcgg cgtcgagggc gcgggcacca tcatgaacat
       61 cgtcgtgctg acggcggcgt tctccagcct caacgccggg ctgtactcga cgggccggat
      121 cctgcggtcg atggcgatga atggcagcgc accgaagttc acctcgctca tgaacaagcg
      181 cggcgtgccc tacggcggta tctgcctgac cgcgctcatc ggattgttcg gcgtgctgct
      241 caacggcatc gtgccgtcgg aggcgttcga gatcgtgctg aacatggcag cgctcggcat
      301 catcgcgtcg tggggcacga tcgtcatctg ccagcttcag ctctaccggt ggtcgaagaa
      361 gggcatcctc gagcggccgt cgttccgatt gtggggcacg ccgtggaccg gatacctcac
      421 actcttgttc ttgcttgcgg tgttggtgct gatggccctc gatccaccga tcggcacctg
      481 gacggtggca tccctgatcg tcatcattcc ggcgttggtg atcggctggt tcgccgtccg
      541 gtccagggta ctggccgtcg cgcaggagcg ccaaggattc acgggttcgt tccctgtggt
      601 ggcgaatcga cctccacccc acgagaagga ccggcgcaag aaggacgact gagccgtcag
      661 gacaactcct cggcccactt cttatggcct tcgaagccaa gggagttcgc catcgcgtgg
      721 acgacgtcgg cgaaaagcac tgtcacttgc ttgtactcgg catgcgtgtc gcgcccgccg
      781 agaggcgaac cgcaaccgtg acatcaccga gcattcgcca acggctcggt cccacacgtc
      841 atgcacgctg ccacggccgt cgtccggccg gtcagacgaa cgcgattgcc agctggacaa
      901 acggactgag cacgttgaac agtccagtcg cgaagtccgc atccaccttg ccgtcgacgg
      961 tctctgaata gagtctgagc gcgacatcga tgatcagata ctgtccgggc ggcactatcg
     1021 cctggtcata gcccaccgag gtgccccgga atacgacggc cgacgtgtcc tcgtcatcgc
     1081 tgaagagccc gctcgaatcc gcgctgacgc gagcgacgcg atacgactgg ccaggctgcg
     1141 gcggaaccga agatattggc tgcgtccatg tctggatgag gtccagggtc ggctcgagat
     1201 ccagtcgcgc cgcgccaccc ccggtgatgc cgccgtccga atgtgccgaa cagaatccgt
     1261 tgagcgccac gatcgcgttg atgttcacca cagcggacgc atcctgtcgg ggattccccc
     1321 acaggtattg gaaatgaagg agctgctcgt caccggccaa gtacgcatcg aagaacgacg
     1381 tgtaatagga cgcttccatc cgcactttcg cctggctgtt atacggctga atcgtcgtcg
     1441 actccacgtt gatgccgtcc gtcgcccaga tctggacggg gctgtccaag gttttgtatt
     1501 gcgtgggcgt atctccagtc gtggcaacca ggccgtcgaa cgccgcgaca gtgccgtcga
     1561 gcagatgccg cagagactcc gcccgcgcac cggactggga gaccgcggcc tgtttgtcct
     1621 ttatcaggcg gtccttgccg cgcgcccgcg ccgcgcccag ttcccgctcc agcgcatcgg
     1681 tgtccatccc catgctgcgg agttgttcgg cgacctgctg tcgcattcgc ctcccctcag
     1741 gcagcagccg ccgaacccct gccgggctcc cggcgacata ggccgaatcg tcatctcgcg
     1801 tcatatgctg cgcggcgctc agcgcctccc gcacgcgccc gttaggcgta ctcacggtgc
     1861 cagccatctc agcctctcct ttcgtcgctg tgaaagaagg ctcctcatat cgtggccctg
     1921 tgatggtcga ccgcaccgcg tattggacta ctcgatttcc tcgggacagt cctcaatgac
     1981 cgatggcgga gcgtcgtcgt taggctgtgc cgcatggctg ctgacatcgt gccgatccgg
     2041 ctcgggttga ccaagggcga cctctacaca ctctgggctc cccgctggcg ggatgccggc
     2101 gacgagtggg aggcgctcct cggcaaggac gacgacctct acggcttcga gtcggttgcc
     2161 gacctggccg ccttcgtccg gaccaacacc gacaacgacc tctccgacca cccggcgtgg
     2221 gaggccacca ccgaggcgaa cgcacatgcg ttcgatccgc cggaggagcg ccagttcgac
     2281 ctgatcggcg tgcaggagct gctcgccgag aagccgaccg aggactccgt ggagacgctg
     2341 caccggacgc tggccatcgt gtcgtccgtc ggatcggtct gcgaactgtc cgcggtcacg
     2401 aagttcttca acggcaatcc cgtgctcggg tcgctgggac tgggcgtcga caccttcagc
     2461 ggcagaagcg gccgcaagcg ctggagcgag atcggcgccg tgatcgggcg gagctgggac
     2521 aacgtcgtcg a
//
LOCUS       DASYLX010001044         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3908680,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001044 DASYLX010000000
VERSION     DASYLX010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908680"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGP27_24465"
     CDS             <1..519
                     /locus_tag="VGP27_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7854488.1"
                     /translation="RAGQLSGGQRQRVSLARATVVPPSVLLCDEPTSALDVSLAASVL
                     NLIRDLRRSLDMSVVFVTHDLSVARVVADRIAVMYLGRIVEIGPAEQVIGAPTHPYTR
                     ALVDSIPDLGRESRVLAGEPASPLSPPTGCPFHPRCPDAVDTCSDVKLDVRLEGTPGN
                     PHQVACIEQKVG"
     gene            519..1385
                     /locus_tag="VGP27_24470"
     CDS             519..1385
                     /locus_tag="VGP27_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854489.1"
                     /translation="MVVALPAPPLARERARLLSMPRFGSDTVNWIGFSLVIVVTVIAV
                     AVPVLAPHDPLLPVGMPLQAPGKGGFLLGTDSVGRDILSRVLFGVRSSWFAALVVVAV
                     GLFIGGLVGVIAGTAGGWLDSLLMRITDGFLSLPAPVLAIAVVAALGPSFAHTLIAVS
                     IVWWPFYARLVRGEVARLAARPHVEAAKLAGVSHIRLAGRHLLPGAVPNALVAASLDL
                     GTLILTLAALSFLGLGQSQPAPELGADSARNLSYFLQQWWVPVMPGLGVLVLALVGNI
                     AGDCLRNLMKTS"
     gene            1399..>2127
                     /locus_tag="VGP27_24475"
     CDS             1399..>2127
                     /locus_tag="VGP27_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854490.1"
                     /translation="MKAFVATRLGAMVAILIALTAVMFLLQHISPLDPVKAQLGAQAS
                     AEAVAQRRAALGLNEPIAVQFWNYLTGAVTGDLGTSYRTRHPVASDLSDFFPATLELA
                     LYGIGIALLLAVVLAFSTTLKWRGAGALRAILFTGASAPMFLLGILGLIVFYQKLGWV
                     PANGRIGIANPPTGPTGLLTIDGLLHGRFDVVADALHHLILPAFVIAIGPAVAIGRVL
                     RSSLLGDSDSDYARTARAKGLSEGQ"
BASE COUNT          277 a          726 c          727 g          397 t
ORIGIN      
        1 cgggccggcc agctgtccgg cgggcagcgg caacgggtgt cgttggcccg cgcgaccgtc
       61 gtgccgccgt cggtgctgct gtgcgacgaa cccaccagcg cgctcgatgt ttcgctggcg
      121 gcgtcggtac tcaacctcat ccgggacctg aggcgcagcc tggacatgtc cgtggtattc
      181 gtcacgcacg acctttcggt cgcccgtgtg gtggccgacc ggatcgccgt gatgtacctc
      241 ggccgcatcg tcgagatcgg tcctgccgaa caggtcatcg gcgcgccgac gcacccctac
      301 acacgggcgc tcgtcgactc gatccccgac ctcggccgcg aatcccgggt gctggcaggc
      361 gaacccgcca gcccgctgtc gccgccgacg ggctgcccgt ttcacccgcg ctgccccgac
      421 gcggtcgaca cctgtagtga cgtcaagctc gacgtccggt tggaaggcac tcccggcaac
      481 ccgcaccagg tggcctgcat cgaacagaag gtgggctgat ggtcgtcgca ctacccgccc
      541 caccgctggc acgtgagcgt gcgcgcctgc tgtcgatgcc gcgcttcggg tcggacacgg
      601 tcaactggat cggcttctcg ctcgtcatcg tcgtcacggt gatcgcggtc gcggtgccgg
      661 tgctggcgcc acacgatccg ctgctgcccg tcgggatgcc cctgcaggcg cccggcaagg
      721 gcggtttcct cctcggcacc gacagtgtcg gtcgcgacat cctctcccgg gtgctgttcg
      781 gtgtccggtc cagttggttc gccgctctcg tggtggtggc tgtcggactg ttcatcggcg
      841 gactggtcgg agtgatcgcg ggcacggcgg gcgggtggtt ggactcgttg ctgatgcgga
      901 tcaccgacgg gtttctgtcg ctgcccgcac cggtgctcgc gatcgcggtg gtcgccgcac
      961 tcggacccag cttcgcgcac acgctgatcg ccgtgtcgat cgtgtggtgg ccgttctacg
     1021 cccggctggt acgcggtgag gtggcccgac tggccgcacg cccgcacgtc gaggcggcga
     1081 aactcgctgg agtcagccac attcggctag ccggccgcca tctgctgccg ggggcggtcc
     1141 cgaacgcctt ggtggccgca agcctcgatc tcggcacgtt gatcctgacc ttggcggcgc
     1201 tgtcgttcct cggtctcggg cagtcccaac ccgcgcctga actgggcgcc gactcggcgc
     1261 gcaatctcag ctacttcctg cagcagtggt gggttcccgt gatgcccggt ctcggtgtgc
     1321 tggtgctcgc gctggtcggc aacatcgcag gcgactgcct gcgcaacctg atgaagacga
     1381 gctaggcaaa gggctgcaat gaaggcgttc gtggcgaccc gcttgggtgc gatggtcgcg
     1441 atcttgatcg cgctgaccgc ggtgatgttc ctgcttcagc acatctcgcc gctcgatccc
     1501 gtcaaggcgc agctaggcgc tcaggcctct gcggaggcgg tggcgcagcg gcgcgcggca
     1561 ctcgggctca atgaaccgat agcggtgcag ttctggaact atctcacggg cgcggtgacg
     1621 ggcgacctcg gcacctccta ccggacccgc catccggttg catcggatct gagcgacttc
     1681 ttccccgcca cactggaact cgccctctac ggaatcggta ttgcactgct gctggctgtg
     1741 gtgctggcct tcagcacgac gctgaagtgg cgcggtgcgg gcgcgctgcg ggcgattctg
     1801 ttcactgggg cgtcggcacc gatgttcctg ttggggatcc tcgggctgat cgtcttctat
     1861 cagaagctgg gttgggttcc ggccaacgga cgcatcggca tcgccaatcc gccgacggga
     1921 ccgaccggtc tgctgacaat cgacggcctg ctgcacggtc gcttcgacgt cgtggcggac
     1981 gccctgcacc acctgatcct gcccgcgttc gtcatcgcca tcgggcctgc ggtcgcgatc
     2041 ggcagggtgc tgcgcagcag cctgctcggc gacagcgaca gcgactacgc gcgtaccgcc
     2101 cgggcgaagg ggctgtccga gggtcag
//
LOCUS       DASYLX010001045         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2156276,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001045 DASYLX010000000
VERSION     DASYLX010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156276"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGP27_24480"
     CDS             <1..795
                     /locus_tag="VGP27_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7854491.1"
                     /translation="DALVADADIVVHLAFIIMGSRDESARVNLAGTRNVFEATVASAR
                     ARRLVYTSSVAAYGYHSDNPVPITEEVPPRGSPEHYYSEQKAACEAALADITADTPLE
                     VYVLRPCIVAGPNAPALAEAMPWNRLPAVVRRVSEALPLLKPPFPDPGTPVQLVHHDD
                     VASAIALAATTSAPTGAYNLAGDGVLSMSDVGEALGARPVPVPLVVAKAASEVIARLP
                     FVPSALEWLHAGRTSVVLDTSKAKSQLGWTPKYTAAETLSGLASAL"
     gene            complement(796..996)
                     /locus_tag="VGP27_24485"
     CDS             complement(796..996)
                     /locus_tag="VGP27_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854492.1"
                     /translation="MKPAATGYPAVVNKEKPETTESDAGAKGADDDVVSDPARSAGAS
                     GDWSDEGGATPSGPADTGDHTQ"
     gene            complement(997..1350)
                     /locus_tag="VGP27_24490"
     CDS             complement(997..1350)
                     /locus_tag="VGP27_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV7854493.1"
                     /translation="MSADVDPAVPPSGTGCAECDAAGGWWVHLRRCAACGHIGCCDDS
                     LARHASAHWRTTRHPIIRSFEPGEDWFWNYQTNEYYDGPELAAPACRPKDETVPGPRG
                     RVPSDWAEQLRRRGD"
     gene            1690..>2024
                     /locus_tag="VGP27_24495"
     CDS             1690..>2024
                     /locus_tag="VGP27_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854494.1"
                     /translation="MNGIAWSRPAFAMGDFVVLAGETFAAAFRRPFALRELLDQIWFV
                     ARVSIVPTLVLSIPYTVLIVFTLNIVLIEVGAGDLSGAGAALASVTQVGPVVTAIVVS
                     GAGATAMCA"
BASE COUNT          299 a          701 c          662 g          362 t
ORIGIN      
        1 gacgcgctgg tcgccgacgc cgacatcgtc gtccacctcg ccttcatcat catgggctcg
       61 cgggacgaga gcgcccgggt caacttggca ggcacccgca acgtgttcga ggcgacggtg
      121 gcgtctgcgc gcgccaggcg gttggtctac acgtcgtcgg tcgccgccta cggctatcac
      181 tccgacaacc cggtgccgat cacagaagaa gtgccgccgc gcggttcgcc cgagcactac
      241 tactccgagc agaaggcggc gtgtgaagcc gcactggccg acatcaccgc cgacacgccg
      301 ttggaggtct acgtactgcg gccatgcatc gtcgcggggc cgaacgcccc ggccctcgcc
      361 gaggccatgc cgtggaaccg gctcccggcg gtcgtccgca gggtgtccga ggccttgccg
      421 ctgctgaagc cgccgtttcc cgacccgggc acaccggttc agctggtgca ccacgacgat
      481 gtggcctccg caatcgcctt ggctgcaacg acttccgcgc caaccggggc ttacaacctg
      541 gcgggcgacg gggtgctgtc catgtccgat gtcggcgaag cgctcggcgc ccgaccggtg
      601 ccggtgcctc ttgtagtcgc gaaggctgcc tctgaggtca tcgcgcgcct gccgttcgtc
      661 ccgtcggcgc tggagtggct gcatgccggg cgcacatcag tggtgctgga cacaagcaag
      721 gcgaagtccc aactcggctg gacgcccaaa tacacggcgg ccgagacgtt gtcgggcttg
      781 gcgtcggcgc tgtagttact gcgtgtgatc cccggtgtcg gccgggccgc tgggcgtggc
      841 cccgccctcg tcggaccagt cgccgctcgc gcccgcgctc ctggcggggt cgctcaccac
      901 atcgtcgtcg gcgcccttgg cacccgcgtc ggactccgtg gtttcgggtt tttccttgtt
      961 cacgaccgcg gggtaccccg tggccgccgg tttcattcag tcgccgcggc gacgcaactg
     1021 ctccgcccag tcgctgggca cccggccgcg cggccctggc acggtctcgt ccttgggtcg
     1081 gcaggccggg gcagcaagct ccggaccgtc gtagtactcg ttggtctggt agttccagaa
     1141 ccagtcctcg ccgggttcga acgaacggat gatcggatga cgggtcgtcc gccagtgcgc
     1201 cgacgcgtgg cgcgccaaag agtcgtcgca gcaaccgatg tgaccacacg ccgcacaccg
     1261 ccgcaggtga acccaccaac cgccggccgc atcgcattcg gcgcacccgg tgccgctcgg
     1321 cggaacggcg gggtcgacgt cggcgctcat agatagtcag cctacagaca cggtgtcggc
     1381 ccgcgtacgg cattaaagat cagcacgatt cgatcagttt gagcgaaacc aaatgcattg
     1441 gggcaacctg cgaattccgc tatagacggg cgggtttggg caatagtcct cgttgacagc
     1501 taataccgtc ccacctactg tccgcgccag cccggcgatt agggaaaggc aatcgcgatt
     1561 tcactccggg aacccgccag ccaaggcagt gctgccgcag ctcctgcccg cccgaagcct
     1621 tgcccgtcga cggcttctgt ctgaagtagg tacttcgatg cagcatgtga gttggcggag
     1681 ccgtcgacca tgaacgggat tgcctggtcc aggcccgcct tcgccatggg cgacttcgtc
     1741 gtactggccg gcgagacatt cgccgccgcg ttccgtcggc cgttcgcctt gcgggagttg
     1801 ctcgatcaga tctggttcgt ggccagggtg tcgatcgtcc ccacgctcgt gctctccatt
     1861 ccctacacgg tcctcatcgt cttcacgctc aacatcgtgc tgatcgaagt aggcgcgggt
     1921 gacctgtccg gtgccggtgc ggcgctggcc tcggtgactc aggtgggtcc cgtggtcacc
     1981 gcgatcgtcg tctccggcgc gggcgccacc gcgatgtgcg ccga
//
LOCUS       DASYLX010001046         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2484459,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001046 DASYLX010000000
VERSION     DASYLX010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484459"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGP27_24500"
     CDS             complement(<1..426)
                     /locus_tag="VGP27_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854495.1"
                     /translation="MGVRMMELLTGSLNALRYEPTAKRVRVFLAGEPVADTMDARLVW
                     EPRRVVPTYAVPATALTTKLLPAGAESGDDETGFRLPDVSELPLLDPSVPFSVHSCAG
                     TAFDVIAGDETAGRAAFQPDDADLADYVVLDFDAFEWREE"
     gene            complement(436..1818)
                     /locus_tag="VGP27_24505"
     CDS             complement(436..1818)
                     /locus_tag="VGP27_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854496.1"
                     /translation="MSFDLTPTGAQHDLARRAHEFAAEVVRPVAADYDARQEFPWPVL
                     EEAAERGFYSPLFYRDLIGDPTGLSLPMFMEELFWGCAGIGLAIVMPALALSAIGQAA
                     SPEQMLQWAPECFGSPGDLKLAALAISEPEGGSDVRNLRTRAKRDGDDWIVDGHKMWI
                     GNGGIANVHVVNAVVDEELGHRGQALFIVPGATPGLEMVRKLDKLGCRASHTAELKFN
                     AVRVPGANLLGGQEKLEHKLARAREAIEGGRHSGSATMGTFEQTRPMVAAQALGIARA
                     AMEYATEYANRREAFGAPIIDNQGIAFPLADLSTGIDAARLLTWRASWMAASGVPMER
                     GEGSMSKLAASEVAVRATERAIQTMGGWGYIKDHPVEKWYRDAKLYTIFEGTSEIQRV
                     VISNALGAADGKPPLHFDLEPSGGPLNRMFGRGTPVRTQVANAALNAKDHLPAPVMQL
                     AMKVLKPPKK"
     gene            1954..>2162
                     /locus_tag="VGP27_24510"
     CDS             1954..>2162
                     /locus_tag="VGP27_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854497.1"
                     /translation="MLSYDAGATSPPLLDETIGDNLAATSATFPDREALIECATGRRW
                     TYSELHEATRRIATALIERGTKPGDR"
BASE COUNT          371 a          752 c          690 g          349 t
ORIGIN      
        1 ctcctcacgc cactcgaatg cgtcgaagtc gaggacgacg tagtcggcga ggtcggcgtc
       61 gtctggctga aacgccgccc gacccgctgt ctcgtcaccg gcgatgacat cgaaagccgt
      121 tccggcacaa ctgtgtaccg aaaagggcac cgacgggtcg agcagcggga gctcgctgac
      181 atctggaagc cggaagccgg tttcgtcgtc gcccgactcg gcgccggcag ggagcagttt
      241 cgtcgtcagc gcggtggcgg gaacggcata ggtgggtacc acccggcggg gctcccagac
      301 caatcgcgcg tccatggtgt cggccaccgg ttcaccggcg aggaagaccc gaactcgctt
      361 ggcggtgggc tcgtaccgca gcgcattgag actgccggtg agcagttcca tcatcctgac
      421 acccatgtcg acacgctact tcttcggcgg cttcagcacc ttcatcgcca gctgcatcac
      481 gggcgcaggc aggtggtcct tggcgttgag tgcggcgttc gcgacctggg tgcgaaccgg
      541 tgtgccgcgc ccgaacatcc gattcagcgg gcctccggac ggttcgaggt cgaagtgcag
      601 cggcggcttc ccgtcggcgg cacccaacgc gttggagatg acgacccgct ggatttcgct
      661 ggtgccctcg aagatggtgt acagcttggc atcccgatac cacttctcca ccggatggtc
      721 tttgatgtaa ccccagccgc ccatcgtctg aatcgcccgt tcggtggccc gaaccgcgac
      781 ctcgctggcg gccagcttcg acatcgagcc ctcgccacgc tccatgggca caccgctggc
      841 ggccatccag gacgcccgcc aggtcagcag acgcgctgcg tcgatcccgg tggacaggtc
      901 ggcgagcggg aaggcgatgc cctgattgtc gatgatcggc gcaccgaagg cctcgcgccg
      961 gttggcgtac tccgtcgcat attccattgc ggccctcgcg atgccgagtg cctgggcggc
     1021 caccatcggc cgcgtctgct cgaatgttcc catcgtcgcg gacccggaat gcctaccgcc
     1081 ttcgatggcc tcgcgcgcac gggcgagctt gtgctcgagc ttctcctgcc ccccgagcag
     1141 gttggcgccc ggcacccgga cggcgttgaa cttcagctcc gcggtgtgcg acgcgcggca
     1201 gccgagcttg tccagcttgc gcaccatctc caaccccgga gtagcgccgg gcacaatgaa
     1261 cagagcctgg ccacgatggc cgagttcctc gtccaccacg gcgttcacca cgtgcacgtt
     1321 ggcgatgccg ccgttgccga tccacatctt gtgaccgtcg acgatccagt cgtcgccgtc
     1381 gcgcttggcc cgggtgcgca agttgcggac gtcgctgccg ccctccggct cggagatggc
     1441 gagggcggcg agcttgagat cgccgggcga accgaagcac tccggcgccc actggagcat
     1501 ctgctcgggt gatgccgcct ggccgatcgc cgacaacgcc aatgcgggca tcacgatggc
     1561 cagcccaatg ccggcgcacc cccagaacaa ctcctccatg aacatcggca gcgacagccc
     1621 ggtgggatca ccgatgaggt cgcgatagaa cagcggactg taaaagcctc gctcggcggc
     1681 ctcctcgagc accggccagg gaaactcctg tcgggcgtca tagtcggcgg ccaccggacg
     1741 gaccacctcg gcggcgaact catgcgcacg tcgtgccaaa tcgtgctgcg cccctgtggg
     1801 cgtcaaatcg aagctcatga cggcgtcacc attattgaca tttccccacc cccgcctttc
     1861 ggatccgaat tacggtgtcg aataccccgc gaccgccgaa aattcacggt gggagccgcg
     1921 gacgcgagga gcagagattg aggagcgcgt cgcatgttgt cctatgacgc cggagccacc
     1981 agcccgccac tgctcgacga gacgatcggc gacaacctgg ccgcaacgtc ggcgaccttc
     2041 cccgaccggg aggcgctcat cgagtgcgcg accggccgcc gctggaccta cagcgagctt
     2101 cacgaagcga ccagaaggat cgccaccgca ctgatcgagc gcggcaccaa gccgggcgac
     2161 cg
//
LOCUS       DASYLX010001047         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_292137,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001047 DASYLX010000000
VERSION     DASYLX010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292137"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGP27_24515"
     CDS             <1..181
                     /locus_tag="VGP27_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7854498.1"
                     /translation="DAAIRQAMTTALHQVQESYEHPAGAYWVPRRMGGSAPTPEEAKV
                     LDEIELAQRGRKQPR"
     gene            213..1028
                     /locus_tag="VGP27_24520"
     CDS             213..1028
                     /locus_tag="VGP27_24520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7854499.1"
                     /translation="MTLPALIASDVDGTLLDDDENISPRTRSVVRAAVDAGVRFVLAT
                     GRPPRWVQPVVDALGFAPMSVCANGAVIYDPATDRIVSSRTLSSDQLAELAEIATRVI
                     PGAGLAVERVGRSAHDAATPQFVSSPGYEHAWLNPDNTEVSVEDLLAAPAIKLLIRKA
                     GARSSDMAAELAKHVGLQGDITYSTNNGLIEVVPLGISKATGVEEVARPYEITAEDVV
                     AFGDMPNDIPLLRWAGLGVAMGNAHPETVDAADEVTAPNTDDGLARVLERWWM"
     gene            complement(1029..>1559)
                     /locus_tag="VGP27_24525"
     CDS             complement(1029..>1559)
                     /locus_tag="VGP27_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEV7854500.1"
                     /translation="RDVGITECPGNAAYAAMDQIRDIAARFNEPLPPPSLTDQLRGGA
                     ILAKWESMGGQNSPLGAPKSLEAAGEGSARYATFDRGAVYWSPQSGAEPLTGAIYDAW
                     ASLGYERGVLGLPTSGEIAEPQWIVQNFQHGTLNFDRENATVTRVIDGVPEELPPLPD
                     DGPPVQLERIKPIDPS"
BASE COUNT          241 a          517 c          541 g          260 t
ORIGIN      
        1 cgacgccgcg attcggcagg cgatgacgac ggcgttgcat caggttcagg agtcctacga
       61 acatccggcg ggcgcgtact gggtaccccg gcggatgggt ggctcggctc cgacgcccga
      121 ggaggccaag gtgctcgacg agatcgagct ggcccagcgc ggccgcaagc agccgcgatg
      181 accgaggcga gaaacgaagg tggatcgccg cgatgacttt gcctgcgctg atcgcgagtg
      241 acgtggacgg cacgctgctc gacgacgacg agaacatcag tccccgcacc cgcagtgtcg
      301 tgcgcgccgc ggtggatgcc ggtgtccggt tcgtgctggc gaccggccgc cctccgaggt
      361 gggtgcagcc cgtcgtggac gcgctcgggt tcgcgccgat gtcggtgtgc gcgaacggtg
      421 cggtgatcta cgacccggcc accgaccgca tcgtgtcttc gcggacgctg tcgtcagatc
      481 agttggcgga gctcgccgag atcgcaaccc gcgtcatccc cggtgcgggc cttgcggtcg
      541 agcgggtggg ccgcagcgcg catgacgcgg cgaccccgca gttcgtcagc tcgccgggct
      601 atgaacacgc ctggctgaac ccggacaaca ccgaggtgtc cgtcgaagat ctactcgccg
      661 cgcccgcgat caagctgttg atccgcaagg cgggcgcgcg cagttcggac atggccgctg
      721 aactcgccaa acatgtcggc ctgcagggcg atatcactta ctcgaccaac aacggcctta
      781 tcgaggtcgt gccgcttggc atcagcaagg ccaccggcgt cgaagaggtc gcacggccct
      841 acgagatcac cgccgaggat gtggtggcct tcggtgacat gcccaacgac atcccgctgc
      901 tgcggtgggc gggtctgggg gttgcgatgg gcaatgcaca ccccgagacc gtcgacgcgg
      961 cggacgaagt caccgcgccc aacaccgacg acggtttggc gcgcgtgctc gagcgctggt
     1021 ggatgtgatc agctcgggtc gatgggtttg atccgctcca gctgtacggg gggaccgtcg
     1081 tccggcagcg gcggcagctc ctcgggtacg ccgtcgataa cccttgtcac cgtggcattt
     1141 tcacggtcga agttcagcgt gccgtgctgg aagttctgca cgatccactg cggctcggca
     1201 atctccccgc tcgtcggcag gcccagcacg ccgcgctcgt atccgagcga cgcccatgcg
     1261 tcgtagatcg ccccggtgag cggttcggcc ccgctctgcg gtgaccagta gaccgcgccc
     1321 ctgtcgaagg tggcgtagcg ggcgctgcct tcgccggccg cttccaacga tttcggtgca
     1381 ccgagagggc tgttctgccc gcccatcgac tcccacttgg ccagaatcgc gccgccgcga
     1441 agctggtcgg tcaggctcgg cggtgggagc ggttcgttga atcgagccgc gatgtcccgg
     1501 atctggtcca tcgccgcata cgccgcgttg cccggacatt cggtgatgcc gacgtcgcg
//
LOCUS       DASYLX010001048         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_619877,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001048 DASYLX010000000
VERSION     DASYLX010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619877"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VGP27_24530"
     CDS             <1..179
                     /locus_tag="VGP27_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV7854501.1"
                     /translation="LPKAGGQEAQFVEGAVNASSVPERDLSVQLSGWMLMEAAHAVTA
                     SAGSAQDAEEDAPA"
     gene            176..2143
                     /locus_tag="VGP27_24535"
     CDS             176..2143
                     /locus_tag="VGP27_24535"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEV7854502.1"
                     /translation="MTEPGGWTPDEDTVERANLTRFMAWLADTGRGEFEDYSQLLAKS
                     LEDIAWFWDAVWHFFDIRATTPPDTVLAGREMPGAQWFPGATLNYAVEAFRHETDEYP
                     ALVAAGEDGSTEWSWARLRSETAAFANYLRRLGVKPGDRVVGYLPNIGEAVAAFLGAA
                     SVGATWAVCNQDLAVEGVVARLGQLEPTVLVASDGSVYAGKRHDRSKELAEIRSKLPS
                     LRATVLVPRLGAEPEEHDESSDVTPWSKVLDLDAPLEITPVPFGHPLWVLFSSGTTGT
                     PKGIVHGHGGVVLEHLKYLSLHADLKPGERFCWYSTTSWMMWNLLVSGLLVGATVVLY
                     DGSPTYPRTDSMWKVVGDHDVALFGAGAGYFLSCAKEGAEPRAKIGEEYRLDSLRGVG
                     STGSPLPAAGFRWIQEAVGRAVPVMSMSGGTDVVTAFIGGCPLVPIVAGELNVICLAA
                     DVQAWTDANTPVVGEAGELVITQPMPSMPVFFWNDADGSRYRKAYFDKYPGIWCHGDW
                     ITITDRDSVVVHGRSDATLNRMGVRMGSAEIYNAVESLAEVQDCLVVGVEKDDGGYWM
                     PLFVHLAGDAELNDELRDKITVAIRKGASPRHVPDDIVDVPGIPRTMTGKRLEIPIKR
                     ILLGARPADVVSASAVDKPDLLDVFAEHARA"
     gene            2194..>3228
                     /gene="nhaA"
                     /locus_tag="VGP27_24540"
     CDS             2194..>3228
                     /gene="nhaA"
                     /locus_tag="VGP27_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEV7854503.1"
                     /translation="MLPTRFSRDPKAKKSGENTAAGLLLLATVIAIAWANSPWSESYW
                     TLLDTHVGFSFGDTHFELTVKHLINDGLMTFFFFVVGLEVMAEFTVGELTDRARAVVP
                     VLAAVAGLAVPALIFLLINAPGENARAWGVVISTDTAFLIGALAIINPKFPARLRLFL
                     LTLAVVDDIGALTVIALFYSDDIRIGPLLVAAVLIAAIALVRYLPAGRGFSYAVIGFA
                     LWVALHSGGVHPTLAGVAIALLIPVFTPERRQVEQTVELIRAFRQSPNSQYARAASRG
                     LRESISINERLQTEFGPYVSFLILPLFALANAGVRLTGETMSAAMRSPLTWGIVGGLV
                     LGKFIGITSAT"
BASE COUNT          504 a         1058 c         1120 g          546 t
ORIGIN      
        1 acctgccgaa ggcgggcggt caggaggccc agttcgtcga aggcgccgtc aacgcgtcct
       61 cggtccccga gcgcgacctg tcggtgcagt tgtcgggctg gatgctgatg gaggcggcgc
      121 acgctgtcac cgcgagcgca gggagcgcac aggacgcaga ggaggacgca ccggcatgac
      181 cgaacccggg ggctggacac cggacgaaga caccgtcgag cgcgccaacc tcacccgctt
      241 catggcgtgg ctggccgaca ccggccgcgg cgagttcgag gactactccc agctgttggc
      301 gaagtcgctg gaggacatcg catggttctg ggacgcggtg tggcacttct tcgacatccg
      361 tgcgaccaca ccccctgaca ccgtgctggc gggtcgggaa atgcctgggg cacagtggtt
      421 tccgggtgca acgctgaact atgccgtcga ggccttccgg cacgagaccg acgagtaccc
      481 ggcgctggtg gcggcgggcg aggacggttc gacggaatgg tcatgggcgc ggctgcgatc
      541 ggagacggcg gcgttcgcga actacctgcg ccggctcggc gtcaagccgg gcgaccgggt
      601 ggtcggatac ctgcccaaca tcggcgaagc ggtcgccgcc ttcctcggtg cggccagcgt
      661 cggcgcgacc tgggcggtgt gcaatcagga cctcgccgtc gagggcgtcg tcgcccggct
      721 cgggcagctc gaaccgacgg tgctggtggc cagcgacggt tcggtctacg cgggcaagcg
      781 tcatgaccgc agtaaggaac tcgccgagat ccgctcgaag ttaccgagtt tgagggcgac
      841 cgtgctggtg ccccgcctcg gcgccgagcc tgaggagcac gacgaaagca gcgacgtcac
      901 gccgtggtcg aaggtcctcg acctcgacgc gccgctggag atcaccccgg tgccgttcgg
      961 ccatccgctc tgggtgctgt tctcctcggg caccaccggc acgcccaagg gcatcgtgca
     1021 cggccacggt ggcgtggtgc tcgaacacct caagtacctg agcttgcacg ccgacctgaa
     1081 acccggcgaa cgcttctgct ggtattcgac caccagctgg atgatgtgga acctgctggt
     1141 gtccggacta ctggtcggcg cgaccgtggt gctctacgac ggcagcccga cctatccgcg
     1201 aaccgacagc atgtggaagg tggtcggcga ccacgacgtt gccctgttcg gcgcgggcgc
     1261 aggctatttc ctgagctgcg ccaaggaggg ggcggaaccc cgcgcgaaaa tcggtgaaga
     1321 ataccggctc gactcgctgc gcggcgtcgg ctcgacgggt tcgccgctgc ccgccgccgg
     1381 cttccgctgg atccaggagg ccgtcgggcg ggccgtgccg gtcatgtcga tgagcggtgg
     1441 caccgacgtg gtgaccgcgt tcatcggcgg ctgccccctc gtcccgatcg tcgcgggcga
     1501 gctcaacgtc atctgcctcg ctgccgacgt ccaggcgtgg acggatgcga acaccccggt
     1561 ggtgggtgaa gcaggcgagc tggtgatcac ccagccgatg ccgtcgatgc cggtgttctt
     1621 ctggaacgac gccgacggca gccgctaccg caaggcgtat ttcgacaagt atcccggcat
     1681 ctggtgtcac ggtgactgga tcaccatcac cgaccgcgat tcggttgtgg tgcacggccg
     1741 ttcggacgcg accttgaacc ggatgggtgt ccggatgggc agcgccgaga tctacaacgc
     1801 ggtcgaatcg ctggccgagg tgcaggactg cctggtggtg ggtgtcgaaa aggatgacgg
     1861 cggctactgg atgccactgt tcgtgcacct cgccggtgac gccgaactga acgacgagtt
     1921 gcgcgacaag atcaccgtcg ccatccgaaa gggcgcatct cctcgtcacg tgcccgacga
     1981 catcgtcgac gtgccgggaa tcccccgcac gatgaccggc aagcggctgg agatcccgat
     2041 caagcgaatc cttttgggtg ccagacccgc cgatgtggtg tcggcgagcg ccgtggacaa
     2101 gcccgacctg ctcgacgtct tcgcggagca cgcccgggcg tgagcgtcga ggcggcagac
     2161 ccgagcggcg gcggcttccc gcgcatcgtg cggatgctgc cgacccgctt cagccgcgac
     2221 ccgaaagcca agaagtcggg cgagaacacc gccgcgggcc tactgctgct ggccacggtc
     2281 atcgccatcg cgtgggcgaa ttcgccgtgg tccgagagct actggacgct gttggacacg
     2341 cacgtcgggt tcagcttcgg tgacacgcac ttcgagctga cggtcaagca tctgatcaac
     2401 gacgggttga tgacgttctt cttcttcgtc gtcggcctgg aagtgatggc ggagttcacg
     2461 gtcggcgagc tgaccgaccg cgccagggcc gtggtcccgg tgctggccgc cgtcgcgggc
     2521 ctcgccgtgc ccgcactgat cttcctgctg atcaacgcgc cgggcgagaa cgcgcgcgcc
     2581 tggggggtgg tgatctccac cgacaccgca ttcctgatcg gtgcgctcgc catcatcaac
     2641 ccgaaattcc ctgcgcgact gcgactgttc ctgctcacgc tggcagtggt cgacgacatc
     2701 ggggcgctga ccgtgatcgc gctgttctac tccgacgaca tccggatcgg cccactgctg
     2761 gttgccgcgg tgctgatcgc ggcgatcgcg ctggtgcgct acttgcccgc gggccgtggc
     2821 ttctcctacg ccgtgatcgg gttcgcgctg tgggtggcgc tccactccgg cggcgtccac
     2881 ccgacgctgg ccggtgtggc gatcgcgctg ctgatccccg tgttcacgcc ggagcgcagg
     2941 caggtcgagc aaaccgtgga gctgatccgg gccttccgcc aatcgcccaa ctcccagtac
     3001 gcccgcgcag cgagtcgcgg cctgcgagag tcgatttcga tcaatgaacg actccagacc
     3061 gagttcgggc cttatgtgtc gttcctcatc ctgccgctgt tcgccttggc caatgccggg
     3121 gtgcggctga ccggagagac gatgtcggcc gcgatgcggt cgccgctgac gtgggggatc
     3181 gtcgggggcc ttgtcctcgg caagttcatc ggcatcacca gtgcgacc
//
LOCUS       DASYLX010001049         5331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_292324,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001049 DASYLX010000000
VERSION     DASYLX010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292324"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VGP27_24545"
     CDS             <1..861
                     /locus_tag="VGP27_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7854504.1"
                     /translation="SGGFLLAFLACFDVGDRVAIASPGYPCYRNILSALGCEVVEIPC
                     GPETRFQPTARMLADLDLPVQGVIVASPANPTGTVIPPDELAAIASWCEASGVRLISD
                     EVYHGLVYEGAPETSCAWETSREAVVVNSFSKYFAMTGWRLGWLLVPHELLRAVDRLT
                     GNFTICPPVLSQLAAVAAFTPESVAEADALLDHYAANRGLLLDGMRKIGIDRLAPTDG
                     AFYVYADVSHLTDDSFSFCSKLLADTGVAIAPGIDFDTQRGSAFVRLSFAGPTGDIEE
                     ALHRIGAWLG"
     gene            complement(865..1611)
                     /locus_tag="VGP27_24550"
     CDS             complement(865..1611)
                     /locus_tag="VGP27_24550"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEV7854505.1"
                     /translation="MTDLGGRTALVTGAASGIGAACARELARRGAVVTVADIDEAAGE
                     ALADDIGGKAWIVDFSDVGPLETLKLETDILVNNAGVQHVSAIAEFPPERFRFIQTLM
                     VETPFLLIRAALPHMYENGFGRIINISSVHGLRASEYKVAYVTAKHALEGLSKVTALE
                     GGPHGVTSNCVNPGYVRTPLVTKQIADQARTHGIPEQEVVEEVLLKESAIKRLLEPEE
                     VASLVVWLASSDAGMVTGASYSMDGGWSAR"
     gene            complement(1708..1881)
                     /locus_tag="VGP27_24555"
     CDS             complement(1708..1881)
                     /locus_tag="VGP27_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854506.1"
                     /translation="MKMKKLGFATVVASGLAAAMLGLAGPAQADIGHHDWVNDIHPTA
                     TVPQVSTSVQQSR"
     gene            2118..2885
                     /locus_tag="VGP27_24560"
     CDS             2118..2885
                     /locus_tag="VGP27_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854507.1"
                     /translation="MPFIDHPKGRAYYRHWAAEDPRAAVVFLHGFGEHTGVYHRYGFA
                     LNAAGIDLWAVDQFGHGLSPGPRGDFGSIADSSGLADTLTELTESERPDVPLIAQGHS
                     FGAVVTLTRLLDQPDRYRAGVISGAPLVPIPELLDADSSLGLDPRALSHDPFYLDTLE
                     NDPLAFVDADGAPLARALDTAWDRFGNDLPGLTVPTLAVHGVADPVAEIGAVRAYADQ
                     LEPLQLKEFPGGRHDILNDTMHRDVARAIIAFVGAKC"
     gene            complement(2882..3433)
                     /locus_tag="VGP27_24565"
     CDS             complement(2882..3433)
                     /locus_tag="VGP27_24565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7854508.1"
                     /translation="MDPRAFRNVLGQFCTGITVITTMHDDAPVGFACQSFAALSLEPP
                     LVLFCPTKVSRSWKAIEASGKFCVNVLHEKQTDVSARFGSKEPDKFAGIDWGSSTLGS
                     PVIKGALAHIDCTVYSVHDGGDHLVVFGAVHSLTDVPHKKPRPLLFYRGQYTGIEPDK
                     NTPAHWRDDLEAFLTATTDDTWL"
     gene            complement(3478..4377)
                     /locus_tag="VGP27_24570"
     CDS             complement(3478..4377)
                     /locus_tag="VGP27_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854509.1"
                     /translation="MSIKSLGYLRIEATDVAAWREYGLKVLGMVEGSGATEGALYLRM
                     DEFPARLVIVPGENDRLLQSGWETANAAALQDIRNRLDVEGTPYKEATAAELADRRVD
                     EMITFDDPSGNTLEVFHGVALEHRRVVSPYGHKFITEEQGLGHVVLTTKDDAESLHFY
                     RDVLGFYLRDSMRLPPQLVGRPADGAPAWLRFFGVNPRHHSLAFMPGETPSGIVHLMV
                     EVGDSDDVGLCLDRALRRKVPMSATLGRHVNDKMLSFYMKTPGGFDVEFGCEGLQVDD
                     HGWVARESTAVSLWGHDFSIGFS"
     gene            complement(4374..5273)
                     /locus_tag="VGP27_24575"
     CDS             complement(4374..5273)
                     /locus_tag="VGP27_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854510.1"
                     /translation="MTSFAAEAAQQQEITFESTSRYAQVRSGSLDMRLHYHEAGVGHE
                     DTVVLLHGGGPGASSWSNFGRNIGVLAKHFHVIAVDQPGYGHSDKHTEHEQYNRYSAT
                     AVLNLFDHLQIDRAALVGNSLGGGTAVRFALDNPERAGRLVLMGPGGLSVNLFAPDPT
                     EGVKLLGRFTAEPTRENMEKFLRIMVFDQKLITPELIDERFAIASQPESLAAARAMGK
                     SFAGPDFELGMMWREVYKLRQRVLLIWGREDRVNPLDGALVALKQIPRVQLHVFGQCG
                     HWAQLEKFDEFNKLTIDFLGGGS"
BASE COUNT          888 a         1750 c         1694 g          999 t
ORIGIN      
        1 tcgggtggat tcctgctggc gttcctcgcc tgcttcgacg tcggcgaccg ggtcgcgatc
       61 gccagccccg gctatccctg ctaccgcaac attctgtcgg cgctcggatg tgaggtcgtc
      121 gagatcccgt gcggacccga gaccaggttc cagccgacag cgcggatgct cgccgacctc
      181 gatctgcccg tacagggcgt catcgtggcg agccctgcca atccaaccgg caccgtgatt
      241 ccgcctgacg agctggcggc gatcgcgtcg tggtgcgagg cctccggtgt tcggctgatc
      301 agcgatgagg tttaccacgg gcttgtctac gagggtgcac ccgaaaccag ctgtgcgtgg
      361 gagacctcgc gggaagccgt tgtggtcaac agcttctcga agtacttcgc gatgaccggg
      421 tggaggctcg gatggcttct ggtgccgcac gaactgctgc gcgcggtcga ccgcctgacc
      481 ggaaacttca cgatctgtcc gcccgtgctg tctcagctgg cagcggttgc ggcgttcacg
      541 ccggagtcgg tcgccgaggc ggacgccctg cttgaccatt acgccgccaa ccgcgggttg
      601 ctgctcgacg ggatgcgcaa gatcgggatc gatcggctcg cgccgaccga cggcgcgttc
      661 tacgtgtatg ccgacgtgtc gcatctgaca gacgactcgt tctcgttctg ttcgaagctg
      721 ctggccgaca ccggggttgc catcgcgccg ggcatcgact tcgacactca gcgcggcagt
      781 gcgttcgtcc ggctgtcgtt cgcgggcccg accggcgaca tcgaggaggc gctgcaccgc
      841 atcggcgcct ggctcggctg atcgtcagcg cgcgctccat ccgccgtcca tgctgtagga
      901 agcaccggtc accatgccgg cgtcggatga tgcgagccaa accacaagcg aggcaacctc
      961 ttcgggttcg aggagacgtt tgatcgcgct ctccttcagt agcacctcct cgacgacctc
     1021 ctgctcgggg atgccgtggg tgcgggcctg atcggcgatc tgcttggtga ccaaaggcgt
     1081 gcggacgtag ccggggttga cgcagttgct cgtcacaccg tgagggccgc cctcgagtgc
     1141 cgtcaccttg gacagtccct cgagcgcgtg cttggcggtg acgtaggcga ccttgtactc
     1201 ggaggcgcgg agaccgtgca ccgaagagat gttgatgatg cgtccgaagc cgttttcgta
     1261 catgtgcggc agcgcagcgc ggatcagcag aaatggtgtc tccaccatca gcgtctggat
     1321 gaatcggaag cgttcgggcg gaaactccgc gatcgcgctc acgtgctgta cgccggcgtt
     1381 gttgaccagg atgtcggttt ccagcttcag cgtctccagc ggcccgacat cggagaagtc
     1441 gacgatccag gccttcccac cgatgtcgtc agccagtgct tctcctgctg cttcatcgat
     1501 atcggcgacg gtcaccacgg ctccgcggcg cgccaattca cgcgcgcagg ctgccccaat
     1561 accgctggct gcgccggtga ccagcgcggt gcggccaccc aagtcagtca cgtcgacgaa
     1621 ggtacccccg tctgcaatgg tcgaaaaccg cgttgaatac gaagaagggg cacccgctat
     1681 ggggtgcccc ttcgtgtggc tagttggtca gcggctctgc tgcacgctgg tgctcacctg
     1741 cggaacggtc gccgtggggt ggatgtcgtt aacccagtcg tggtggccga tatcggcctg
     1801 cgccggacca gccaagccga gcatggcggc ggccagacca cttgcgacca ctgtggcaaa
     1861 cccaagcttc ttcattttca cgccttcttc ttttttcctg tggtatccgg tcctcggcgg
     1921 tccgaactcc gtatcgccgg tctttgcgga tctttctaac cggtctgatt gcataaacag
     1981 gcaggtcaca tgtttaattc cgttggcagc aattgatcgg cagttggcag gaaccgactg
     2041 cccggcacgc gcgcaacgcc ttcgcaggcg tcatttgcca tggctttgtg cagtgggtca
     2101 cagctaaact cgggcttatg ccgttcatcg atcatccgaa gggtcgggcc tactaccgcc
     2161 actgggcggc cgaagaccca cgcgcagccg tcgtcttcct gcacgggttc ggcgaacaca
     2221 ccggcgtgta ccaccggtac ggcttcgcgc tcaacgccgc ggggatcgac ctgtgggcgg
     2281 tcgaccagtt cggccacggc ctgagcccgg gcccgcgcgg tgacttcggc tccatcgcag
     2341 acagctccgg cctcgccgac accctgaccg agctgaccga gagtgaacgg ccggatgtgc
     2401 cgctgatcgc ccagggccat tcgtttgggg cggtggtgac gctgacgcgg ttactcgacc
     2461 aacccgaccg ctaccgcgcg ggcgtcatct ccggcgcgcc gttggtgccg atcccggagc
     2521 tgctcgacgc ggacagctcg ttgggtctgg acccacgcgc gctttctcac gacccgttct
     2581 atctggacac gctggagaac gacccgctgg cgttcgtgga cgccgacggt gcgccgttgg
     2641 cacgcgcgct cgacacggcg tgggaccgct tcgggaacga tctgccgggg ctgacggtgc
     2701 ccacattggc ggtgcacggc gtcgccgatc ccgtcgccga gatcggcgcg gtgcgcgctt
     2761 atgccgatca gttggaaccg ctgcagctca aggagtttcc cggtgggcgc cacgacatcc
     2821 tcaacgacac catgcaccgc gacgtggcac gcgcgatcat cgccttcgtc ggcgcgaagt
     2881 gttagagcca ggtgtcgtcg gtggtggcgg tgagaaacgc ctcgaggtcg tcgcgccagt
     2941 gcgcgggcgt gttcttgtcc ggttcgattc ccgtgtactg gccgcgatag aacagcagcg
     3001 gccgcggctt cttgtgcggc acatcggtca atgagtgcac cgcaccgaaa acgacgaggt
     3061 ggtccccccc gtcatggacc gaatacaccg tgcagtcgat gtgcgccagc gcgcccttga
     3121 tcaccggaga gcccaatgtt gatgagcccc aatcgattcc ggcgaacttg tccggttcct
     3181 tggaaccgaa tcgtgccgag acatccgtct gcttctcgtg caacacgttc acacagaact
     3241 tgccggacgc ttcgatggcc ttccaggacc gcgacacctt ggtcgggcag aagagaacca
     3301 gcggcggctc cagcgacagt gccgcaaacg actggcaggc gaaaccaacc ggtgcatcgt
     3361 cgtgcatcgt ggtgatgacc gtgatgccgg tgcagaactg cccgagcaca tttcggaatg
     3421 cgcgagggtc catcgcttct tcgctcctcg cgtgcgcggg cggctcgccg gtcacgtcta
     3481 ggagaagccg atgctgaagt cgtgacccca caggctgacc gcagtgctct cccgcgcgac
     3541 ccagccgtga tcatcgacct gcaaaccctc acaaccgaat tcgacgtcga acccgccggg
     3601 ggtcttcatg tagaacgaca gcatcttgtc gttcacgtgc ctgcccagcg tcgccgacat
     3661 cggcaccttg cggcgcagcg cgcggtcgag gcacaggccg acgtcgtcgg agtcacccac
     3721 ctcgaccatc aggtgcacga tgccgctcgg cgtctccccc ggcatgaagg ccaggctgtg
     3781 gtggcgcgga ttgacgccga agaaccgtag ccacgcgggc gcgccgtcgg cgggcctgcc
     3841 gacgagctgc ggtggcagtc gcatcgagtc gcgcaggtag aaaccgagca catcgcggta
     3901 gaagtgcagc gactcagcat cgtccttcgt ggtcagcacc acgtgaccga ggccttgttc
     3961 ttcggtgatg aacttatggc cgtaggggct gacgacccgg cggtgttcga gcgcgacgcc
     4021 gtggaacacc tccagggtgt tgcctgatgg gtcgtcgaac gtgatcatct cgtcgacccg
     4081 gcgatcggcc agttcggcgg ccgtcgcctc cttgtatggc gtgccctcta cgtcgagccg
     4141 gttacggatg tcctgcagcg ccgcggcgtt cgccgtctcc cagccggatt gaagcagccg
     4201 gtcgttctcg cctggaacga tgaccagccg agcgggaaac tcgtccatcc ggagatagag
     4261 cgcaccttcg gtggcccctg agccttccac catgccgagg accttcagcc cgtactcgcg
     4321 ccatgccgcg acgtcggtgg cctcgatccg caggtagccc agagatttga tgctcatgac
     4381 ccacctccga ggaagtcaat agtgagcttg ttgaactcgt cgaacttctc cagctgcgcc
     4441 cagtgtccgc actgtccgaa gacgtgcagc tgcacacgcg gaatctgttt gagcgcgacc
     4501 agcgcaccgt cgagcgggtt gacgcgatcc tcccgacccc agatcagaag cacccgctgc
     4561 cgcaacttgt agacctcgcg ccacatcatg ccgagttcga aatcgggacc cgcgaacgac
     4621 tttcccattg cccgcgcagc cgcgagcgac tccggctggc tggcgatcgc gaaccgctcg
     4681 tcgatcagct ctggcgtgat gagcttctgg tcgaagacca tgatgcgcag gaacttctcc
     4741 atgttctcgc gcgtcggctc ggcggtgaac ctgcccagta gcttgacgcc ctcggtcggg
     4801 tcgggcgcga acaggttgac cgacagcccg cccggcccca tcaacaccaa tctgcctgcc
     4861 cgctcggggt tgtccagcgc gaatcggaca gcggtaccgc cgcccaacga attgcctacc
     4921 agcgcagcgc gatcgatctg aaggtgatcg aacagattga gcaccgcggt cgcgctgtag
     4981 cggttgtact gctcatgttc ggtgtgcttg tcggaatgtc cgtagcccgg ctggtcgacc
     5041 gcgatcacgt ggaaatgctt ggccagcacg ccgatgttgc gtccgaagtt cgaccagctc
     5101 gacgccccgg gcccgccgcc gtgcagcagc accactgtgt cctcgtggcc gacaccggcc
     5161 tcgtgatagt gcagccgcat atccaaagag cccgagcgga cctgcgcata ccgggaggtc
     5221 gactcgaacg tgatctcttg ctgttgagcg gcttcggcgg cgaatgacgt catcagacca
     5281 tcgtgtcctg gggcggcagc ccgaactcgt tgttaccgaa tatcaggtag g
//
LOCUS       DASYLX010001050         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_731322,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001050 DASYLX010000000
VERSION     DASYLX010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731322"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /gene="cysS"
                     /locus_tag="VGP27_24580"
     CDS             <1..1329
                     /gene="cysS"
                     /locus_tag="VGP27_24580"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7854511.1"
                     /translation="VSIYLCGATVQGLPHIGHVRSGVAFDVLRRWLMAKGFDVAFIRN
                     VTDIDDKILNKAADAGRPWWEWAATFERAFAAAYDALGVLPPSAEPRATGHITQMIEL
                     IARLIDGKHAYAADGDVYFDVLSLPDYGKLSGHRIDDVHQGEGTTSGKRDQRDFTLWK
                     GEKPGEPSWPTPWGRGRPGWHTECVAMCEAYLGAEFDIHAGGMDLIFPHHENEIAQAE
                     AVGDQFARFWLHNGWVTMGGEKMSKSLGNVLAIPAVLQRVRPAELRYYLGSAHYRSML
                     EFSETALQDAAKAYTGIEDFLHRVRNRVGAVEPGEWTPAFAAALDDDLSVPIALAEVH
                     AARAEGNRALEAGDHDTAMARARSIRAMMGILGCDPLDERWESRDEASAALAAIDVLV
                     HAETQRRQDARERKDWAEADAIRDRLKRAGVEVTDTADGPQWTLLDGYTT"
     gene            1330..2277
                     /gene="rlmB"
                     /locus_tag="VGP27_24585"
     CDS             1330..2277
                     /gene="rlmB"
                     /locus_tag="VGP27_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV7854512.1"
                     /translation="MAGNSQRRGAIRKAGTKKGPKVGSGGVRRRGLEGRGATPPAHMR
                     PNHPAAKRAKTQQKTQQQRRPQRRTDDTEVVLGRNPVLECLRAGVPATALWVALGAEA
                     DERLTESVRRAADAGISILEVPRGDLDRISANGLHQGIALQVPPYSYAHPDDLLAAAS
                     ADGAPALLVALDNISDPRNLGAIVRSVAGFGGHGVLIPQRRSASVTAVAWRTSAGAAA
                     RLRVARATNLTRTLKSWRDAGLQVIGLDADSDITLDELDGTDPMVVVVGSEGKGLSRL
                     VRENCDTVVSIPMAGPTESLNASVAAGVVLAEIARQRRG"
     gene            complement(2274..3080)
                     /locus_tag="VGP27_24590"
     CDS             complement(2274..3080)
                     /locus_tag="VGP27_24590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV7854513.1"
                     /translation="MRLANRRVIRKIGAVAAFTRYVALGDSFTEGVGDPHPSSPNGLR
                     GWADHVAVALAQRNPDLRYANLAVRGRRMDEILDEQVQTAVMFEPDLVTIYAGMNNLL
                     LLRNDIDAMMARYAEGLKKLQQTGATVLAFTAADLGTVPLFRRLRGRAAVYNELLRGI
                     ADDLDLALVDFWRFAEFRDSRLWDGDRIHLSPLGHERFAAKVLDALAVSHELTSRSAA
                     SVSSTAPARDFRSNLRWTAAFAAPWIAKRMRLAKPGAGIEPKRTTLSKVV"
     gene            complement(3138..4379)
                     /locus_tag="VGP27_24595"
     CDS             complement(3138..4379)
                     /locus_tag="VGP27_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV7854514.1"
                     /translation="MELTLALVALAVVLVFAMVKPRGWPEAVVAIPAAALLIAAGVVP
                     LRDAADEVGRLLPVVAFLATVLVLARLCDDEGLFVAAGAAMARSAGGNTRRLLLRVFV
                     IAAVTTAVLSLDATVVLLTPVVLATTRALWVPAEPHAYATAHLANTASLLLPVSNLTN
                     LLAFTAAGLSFVHFTAVMAPSWLAAVVAEYFLLRWLFARDLAVKPHPEPTTDPVKVPV
                     FVLVVLALTLAGFAVTSMLGFSPAWAALAGAVILGVRALARRETSVARIAQAADVPFL
                     VFVLCLGVVVDGLMLHGLESAMRGVLPGGTTLLALLGTAAVAAVLSNMVNNLPAVLVL
                     LPLVSASGPAAVLAMLIGVNIGPNLTYVGSLANLLWRSVVRRDIPVGFREFSRVGLIT
                     TPVTLIVAVLGLWAGIRLFGV"
     gene            complement(4370..>4872)
                     /locus_tag="VGP27_24600"
     CDS             complement(4370..>4872)
                     /locus_tag="VGP27_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7854515.1"
                     /translation="IALGDYDVTLVEAQHCPPDRFPGVITTPVRPPVRTSAYKCGEAW
                     STLVHHRPSDRRLLIIGSAGYVPGALAGYRAEVLYLGIGQLGVQPERYLVDYWAETVR
                     TVGARRVVLIHWDDFFRPLTKPLRALPYAGDDLDVSMRVLTALADDDGIPLHLPSLWQ
                     RADPWS"
BASE COUNT          868 a         1635 c         1695 g          674 t
ORIGIN      
        1 gtgtcgatct acctgtgcgg tgccaccgtg cagggtctgc cgcacatcgg tcacgttcgc
       61 agcggagtgg ccttcgacgt gctgcgccgc tggctgatgg cgaagggttt cgacgtcgcg
      121 ttcattcgca acgtcaccga catcgacgac aagatcctca acaaggccgc cgacgcgggc
      181 agaccgtggt gggagtgggc ggccaccttc gagcgggcgt tcgccgcggc ctacgacgcg
      241 ctgggtgtgc tgccgccgtc ggccgaaccg cgcgcgaccg ggcacatcac ccagatgatc
      301 gagttgatcg cccggttgat cgacggcaag cacgcatatg ccgccgacgg cgacgtctac
      361 ttcgatgtgt tgagcctgcc cgattacggc aagctgtcgg gccaccggat cgacgacgtc
      421 catcagggcg aaggcaccac atccggcaag cgcgaccaac gcgacttcac attgtggaag
      481 ggcgagaagc ccggcgagcc gtcctggccg acgccgtggg gtcgcggaag gccgggctgg
      541 cacacggaat gcgtcgcgat gtgcgaggcg tacctcggcg ccgagttcga catccacgcg
      601 ggcgggatgg atttgatctt cccgcaccac gagaacgaga tcgcgcaggc cgaggccgtc
      661 ggcgaccaat tcgcccggtt ctggctgcac aacggctggg tcaccatggg cggcgagaag
      721 atgagcaagt cgctgggcaa cgtgttggct atcccggctg tgctgcaacg ggtccggccc
      781 gcggagctgc ggtactacct gggcagtgcg cactaccggt cgatgctgga gttctccgag
      841 accgcactgc aggatgcggc gaaggcctac accggtatcg aggacttcct gcatcgggtg
      901 cgcaaccggg tcggtgccgt ggagcccggc gagtggacgc ccgcgttcgc cgccgccctc
      961 gacgatgatc tgtcggtgcc catcgcgctc gcggaggtgc acgccgcacg tgccgaaggc
     1021 aacagggcgc tcgaggcagg cgaccacgat acggcgatgg cccgcgcacg ctcgatccgc
     1081 gcgatgatgg gcatcctcgg ctgtgacccg ctcgacgaac ggtgggagtc tcgcgacgag
     1141 gcatccgcgg cgttggctgc catcgatgtg ctggtgcacg cggagacgca gcgtcgccaa
     1201 gatgcacgcg agcgcaagga ctgggcggag gccgacgcga tccgtgaccg gctcaagagg
     1261 gcgggcgtcg aggtcaccga caccgcggac ggaccgcagt ggacgctgct ggatgggtac
     1321 acgacgtaga tggccggaaa ctcgcaacgc cgcggcgcga tccgcaaggc gggcaccaag
     1381 aagggcccca aggttggctc gggcggcgtc cgccgccgcg gcctggaggg ccggggcgcc
     1441 accccgcccg cccatatgcg accgaaccac ccggccgcca agcgcgcgaa gacgcaacag
     1501 aagacgcagc aacagcgccg ccctcagagg cgcaccgacg acaccgaggt cgtgctgggc
     1561 cgcaacccag tgttggaatg cctgcgcgcc ggtgtgcccg ccaccgcgct gtgggtggcg
     1621 ctgggcgccg aggccgacga gcgactgacc gaatcggtga gacgcgcggc cgatgccggg
     1681 atctcgatcc tcgaggtacc ccgcggcgat ctggaccgca tcagtgccaa cgggctgcac
     1741 caaggcatcg cgctgcaggt gccgccctac tcctacgcac accccgacga tctgctcgcg
     1801 gccgcatccg cggacggcgc gcccgcgttg ttggtcgccc ttgacaacat ctccgacccg
     1861 cgcaacctgg gagcgatcgt gcgttcggtg gcaggattcg gcggccacgg ggtgctgatt
     1921 ccgcagcgcc ggtcggcgtc ggtcaccgcg gtcgcatggc gcaccagcgc gggcgccgcc
     1981 gccaggctgc gggtggccag ggcgacgaat ctcactcgga cactgaagag ttggcgtgac
     2041 gctgggctac aggtgatcgg cttggacgcg gacagcgaca tcacgctcga tgagctggac
     2101 ggcacagacc cgatggtcgt ggtcgtcggc tccgaaggca aaggcctgtc gcggctggtg
     2161 cgggagaact gcgacacggt ggtgtccatc ccgatggcgg ggccgaccga gtcgctcaac
     2221 gcgtcggtgg ccgcgggcgt ggtgctggcc gaaattgccc gccagcgacg gggttaaacg
     2281 accttgctca gggtcgtgcg cttcggctcg atgcccgcgc ccggcttggc cagccgcatc
     2341 cgcttggcga tccacggcgc ggcgaatgcc gcggtccagc gaaggttcga gcgaaagtcg
     2401 cgcgccggcg ccgtcgacga caccgacgca gccgaacgcg atgtgagttc gtgggagaca
     2461 gcaagcgcgt caagaacttt ggcggcgaag cgctcgtggc cgagcggtga caggtggatg
     2521 cggtcgccgt cccacagccg cgagtcgcga aattcggcga accgccagaa atcgaccagc
     2581 gcgagatcga gatcgtcggc aatgccccgc agcagttcgt tgtagaccgc ggcgcggccc
     2641 ctcagccgcc ggaacagcgg taccgtgccg aggtcggccg cggtgaatgc gagcaccgtc
     2701 gcacccgtct gctgcagttt cttgaggccc tcggcgtatc tggccatcat ggcgtcgatg
     2761 tcgttgcgca gcagcagcag gttgttcatc cccgcgtaga tggtgacgag atctggctcg
     2821 aacatgacgg cggtctgaac ctgctcgtcg aggatttcgt ccatcctcct gccgcggacg
     2881 gccaggttgg cgtagcgaag gtcgggattt ctctgtgcca gcgcgactgc gacgtgatcc
     2941 gcccatccac gcaagccgtt ggggctcgag gggtgtgggt cgccgacacc ctcggtgaac
     3001 gagtctccga gcgccacgta gcgagtgaag gcggccacgg ctccaatttt gcggattacc
     3061 cgccgattcg ctaatcgcat cgcggtcgat caacgactgg acgtcaaccg tcccacggtt
     3121 gttcgcggcg ccgtaaccta aacgccgaac aaccggatgc ctgcccacag gcccagcaca
     3181 gcgacgatga gggtgaccgg tgtcgtgatc agcccgaccc ggctgaattc gcggaacccg
     3241 acgggaatgt cgcggcggac aacgctgcgc cacagcaggt tcgccagcga gccgacgtag
     3301 gtcaggttgg gcccgatgtt gacgccgatc aacatcgcca gcactgccgc cggtcccgat
     3361 gcgctcacca gcggcagcag cacgagcacc gcgggcagat tgttgaccat gttcgagagc
     3421 accgccgcga ccgccgctgt cccgagcagc gcgagcaacg tggtgccgcc gggcagcacg
     3481 ccgcgcatcg cggattcgag accgtgcaac atcagtccgt cgacaacaac gccgaggcag
     3541 agcacgaaca ccaggaacgg cacgtcggca gcttgggcga tacgggcgac ggacgtctcg
     3601 cgccgagcga gcgcgcgcac gccgagaatc accgcacccg ccagcgcggc ccaggcgggc
     3661 gagaacccca gcatcgatgt gacggcgaag ccggccaacg tcagtgccag caccaccagt
     3721 acgaacaccg ggaccttcac cggatcggtg gtcggctcgg ggtgcggctt cacggcgagg
     3781 tccctggcga aaagccaacg cagcaggaag tattcggcga caacggcagc caaccacgat
     3841 ggcgccatca ccgcggtgaa gtggacgaac gacagtcccg cggcggtgaa cgccagcagg
     3901 ttggtcagat tcgacaccgg cagcagcagc gacgcggtgt tggccagatg cgcggtggcg
     3961 tatgcgtgtg gttcggcagg cacccacagc gcgcgcgtcg tcgcaagcac cacgggcgtc
     4021 aacagcacga cggtcgcgtc aaggctgagc accgccgtcg tgacggccgc gatgacgaac
     4081 accctcagca gcagtcgccg ggtgttccca ccggcggagc gggccatcgc cgcgcctgcc
     4141 gccacgaaca gtccctcgtc gtcgcacaac cgcgccagca cgagcacggt ggcgaggaac
     4201 gcaacaacgg gcagcaaccg cccgacctcg tcggccgcat cgcgcagcgg caccacaccg
     4261 gccgcgatca gcaacgccgc ggcgggaatg gccactacgg cttccggcca gcctcgcggc
     4321 ttgaccatcg cgaacaccag cacaaccgcc agcgccacca gcgcgagcgt cagctccacg
     4381 ggtcagcgcg ctgccacagc gagggcagat gcagcggtat gccgtcgtcg tcggcgagtg
     4441 cggtgagcac ccgcatcgag acgtcgaggt cgtcgcctgc gtagggaagg gcgcgcagcg
     4501 gcttggtcag tggccgaaag aagtcgtccc agtggatcag aaccacgcgg cgtgcgccga
     4561 cggtccgcac cgtctcggcc cagtagtcga cgaggtagcg ctccggttgc acacccagct
     4621 gcccgatgcc gagatagagg acctcggcgc ggtatccggc caacgcgccg ggaacgtatc
     4681 ctgcgctgcc gatgatcagc agccgtcgat ccgacggtcg gtggtgcacc aacgtcgacc
     4741 acgcctcacc acacttgtag gccgacgtcc tgaccggagg cctgacgggc gtggtgatca
     4801 cacccggaaa ccggtccggg gggcagtgtt gcgcttccac cagcgtcacg tcgtagtcgc
     4861 cgagcgcgat gg
//
LOCUS       DASYLX010001051         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_840798,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001051 DASYLX010000000
VERSION     DASYLX010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840798"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGP27_24605"
     CDS             <1..488
                     /locus_tag="VGP27_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854516.1"
                     /translation="RGWMAGICAGLLALTIAAVVGGVLLIRDDRDIQEVNRNDAAALQ
                     AAKDCVTALQAPDTAAMNAAQTKIVECSTGAFGAQASTFGGILVEAYQAAKVKVAVSD
                     MRAAVERHDPSGSVDVLVAVRIKVTNTAAADQESGYRLRLTMTPDQGQYRIDKMEQVT
                     S"
     gene            485..1462
                     /locus_tag="VGP27_24610"
     CDS             485..1462
                     /locus_tag="VGP27_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7854517.1"
                     /translation="MTAVLDIRADAAVGGSSPTQALASWPARAGALCLDVLLGIGVLA
                     VLVPLVWTAPREGWLWWVYMAVAAVTVLLLLANRWLLPAVTGWSLGRALFGIRVVRRG
                     NGADVGFGRLLARDFVHLLDTAALFIGWLWPLWDPRNRTFADLLLRTEVRRVQPPERD
                     MRRFTAKVLAAVVVVCAALVASNYVFVYRHERAVDQARQQITDEGPRIVEQMLTYSPD
                     TLKDDFARAQSLATEGYRSQLVAQQEAAQKAGPTANEYWSVSSAVTNVAADRATMLLA
                     MQGQRGADPNTLRFITATVLVDFDKSRDGKWRVASLTVLKKPFMGQAPQ"
     gene            1459..2010
                     /locus_tag="VGP27_24615"
     CDS             1459..2010
                     /locus_tag="VGP27_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEV7854518.1"
                     /translation="MSPRRKIDAEEPELYTAPPPKPPRRWGLPLVATVASVLVAAAIA
                     VSTLMLVSHQTDRTTAAKDADVTGYVKSFMTTYTTLDPFQANYYGDRIGSQATGDFAK
                     MFKEKINEILIQVARAEPTQGNVVAAGVQRWNDDGSADVLVATNVSSKMPDGKTLESG
                     NRWVVTAKKEGQLWKISQLIQVI"
     gene            2058..2969
                     /locus_tag="VGP27_24620"
     CDS             2058..2969
                     /locus_tag="VGP27_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEV7854519.1"
                     /translation="MPSGKKLRTGNTEPADSQAAPEVTETTAEEPAAVEEPTSTVETA
                     VTTVDEPVEDPVQEPVQEPPAKTAKKGRRNLLRRRKNEPEVVGEATESAGEATESTEE
                     AATEPTEASQAERPADGDGPEREPVLVPHQPAGKRLKIAAAAAAVLFVAGGAFAGATA
                     QPVIANRALVETKLNIARTAANAITTLWSYTPENMDSLADRSARYLTGDFAFQYRKFI
                     DSIVATNKQAQVTNTTSVLGAAVESVNQSEAAAVVYTNSVATSPVSKNIPSLRYLSYR
                     LEMKRDRSDWRITRMTTITTLDLTPQL"
BASE COUNT          545 a          976 c         1039 g          445 t
ORIGIN      
        1 gccgtggatg gatggccgga atctgcgccg gcttgttggc gctgacaatt gccgcggtgg
       61 tgggcggtgt tctcttgatc cgcgacgacc gcgatatcca agaggtcaac cgcaatgatg
      121 cggcggcact gcaggccgcc aaggactgcg tgaccgcgct gcaggccccc gacaccgctg
      181 cgatgaacgc ggcgcagacg aagatcgtgg agtgctccac cggcgcgttc ggagcgcagg
      241 cctcgacgtt cggaggcatc ctggtagagg cctatcaggc cgcgaaggtc aaggttgcgg
      301 tgtccgacat gcgggcagct gtcgaacgcc acgaccccag cgggtcggtg gacgtgctgg
      361 tggcggtgcg gatcaaggtg accaacaccg ccgccgccga tcaggaatcc ggctaccggc
      421 tgcgcttgac gatgacgccc gaccagggtc agtaccggat cgacaagatg gaacaggtca
      481 cgtcgtgacg gctgttctcg acatcagggc ggacgcggcg gtcggcggtt cgtcaccgac
      541 gcaggcgctg gcgtcgtggc cggcccgtgc gggcgccctc tgtctggacg tactgctggg
      601 catcggtgtg cttgcggtgc tggtgcccct cgtgtggacc gcgccgcgcg agggctggct
      661 ctggtgggtc tacatggccg tcgcggccgt gacggtcctg ctgctcctcg ccaaccgctg
      721 gctgctgccc gccgtcaccg gatggagcct cggccgggcg ctgttcggga tcagggtcgt
      781 gcggcggggc aacggcgccg acgtcggttt cgggcggttg ctggcgcgcg acttcgtcca
      841 cctgttggac accgcggcgc tcttcatcgg atggctgtgg ccgctgtggg acccccgcaa
      901 ccgcaccttt gcggacctgt tgttgcgcac cgaggtccgg cgggtgcaac cgccggagcg
      961 cgatatgcgc cggttcaccg cgaaggtgct ggcggccgtc gtggtggtgt gcgccgccct
     1021 ggtggcatcg aactacgtct tcgtctaccg ccacgaacgg gcggtagatc aggcccgtca
     1081 gcagatcacc gacgaaggcc cgcggatcgt cgaacagatg ttgacctaca gccccgacac
     1141 cctgaaggac gatttcgcgc gggcgcagtc gctggcgacg gagggttacc ggtcccaact
     1201 ggtcgcccag caggaggcgg cacagaaggc cgggccgacg gcgaacgagt actggtcggt
     1261 cagcagtgcg gtcaccaatg tggcggcgga tagagcgacg atgctgctgg ccatgcaggg
     1321 ccagcggggc gccgacccga acaccttgcg tttcatcacc gcgacggtgc tggtcgactt
     1381 cgacaagtcc cgtgacggca agtggcgggt ggccagtctc accgtgctca agaagccgtt
     1441 catgggtcag gctccgcaat gagcccgcgc cgcaagatcg acgccgagga gccggagctc
     1501 tacaccgctc cgccgccgaa acccccacgg cgatgggggc ttccgctggt ggcgacggtc
     1561 gcaagcgtgc tcgtcgcggc ggcgatcgcg gtgagcacct tgatgctggt ttcgcatcag
     1621 accgaccgca cgacggcggc caaggacgcc gacgtcaccg gttacgtgaa gtcgttcatg
     1681 accacgtaca cgacgctgga cccgttccag gccaactact acggcgaccg aatcggatcg
     1741 caggccaccg gcgacttcgc gaagatgttc aaagagaaga tcaacgagat cctcatccag
     1801 gtggcccgcg ccgaacccac gcagggcaat gtcgtcgccg ccggcgttca gcgctggaac
     1861 gacgacggca gcgccgacgt tctggtcgcc accaacgtct cgagcaagat gccagacggc
     1921 aagacgcttg aaagcggaaa ccgttgggtc gtaacggcga agaaggaagg gcagctgtgg
     1981 aagatcagcc aactgattca ggtgatctga ccacggatgc ggctgcggca ccgtcgacgc
     2041 cccgcggtcg acaccggatg ccgagcggca agaagctgcg caccggcaac accgagcccg
     2101 ccgattcgca ggccgcgccc gaagtcaccg aaaccaccgc cgaagagccg gccgcggtcg
     2161 aagagcccac atcgacggtg gagacggcgg tgacgacggt cgacgagccg gtcgaggacc
     2221 cggttcagga accggttcag gaaccgcccg ctaagaccgc caagaagggc cggcgaaatc
     2281 tgctgcgacg gcgcaaaaac gagcccgaag tggttggcga ggcaaccgaa tcggcgggcg
     2341 aggcaaccga atcgacggaa gaagccgcca cggagcccac cgaggcctcg caggccgagc
     2401 ggcccgcgga cggcgacggg ccggagaggg agccagttct cgtcccccac cagccggcag
     2461 gcaagcggct gaagatcgcc gccgcagcgg cggctgtgct gttcgtggcg ggcggcgcgt
     2521 tcgcaggcgc gacggcgcaa ccggtcatcg ccaaccgcgc gctcgtcgag acgaagctga
     2581 acatcgcgcg tacggcggcg aatgcgatca cgacgctgtg gtcctacaca ccggagaaca
     2641 tggactcgct tgccgaccgg tcggcgcgct atctcaccgg cgacttcgct ttccaatacc
     2701 gcaagttcat cgattcgatc gtcgccacca acaaacaggc tcaggtgacg aacaccacct
     2761 cggtgctcgg tgcggcggtg gaatcggtga accagtcgga ggcagccgcc gtcgtataca
     2821 cgaattccgt tgcgacgagc ccggtgtcga agaacattcc gtccctgcgc tacttgtcgt
     2881 accgattgga gatgaagcgg gaccggtcgg actggcgcat cacccggatg acgacgatca
     2941 ccacgctgga cctcaccccg cagctgtagg cggacgccgt gcccgtcacc tctccggtct
     3001 cacat
//
LOCUS       DASYLX010001052         9776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_621219,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001052 DASYLX010000000
VERSION     DASYLX010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621219"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..186
                     /locus_tag="VGP27_24625"
     CDS             1..186
                     /locus_tag="VGP27_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854520.1"
                     /translation="MAAIAVAPVAAAAPQCTNTGPNTTQCETNGSAQIVTSPPANNNY
                     GYWGYPIIGFGGWGFGI"
     gene            246..1487
                     /locus_tag="VGP27_24630"
     CDS             246..1487
                     /locus_tag="VGP27_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV7854521.1"
                     /translation="MEGGVLLRDDKTRMRPSWLLLGPAFVAAIAYVDPGNVAANISAG
                     AQYGFLLVWVIFLANAMAGLVQYLSAKLGLVSGRTLPEAVADHSRASTRIAFWLQAET
                     VAMATDLAEVVGGAIALNLLFDLPLLIGGVITGAVSLLLLAIQNRRGQRVFERVISGL
                     LLIIAIGFTTSLFVEPPPLADAAEGLVPRFAGAESVLLATAMLGATVMPHAVYLHSGL
                     ARDRHGHPEPGARRTWLLRVTRIDVGLAMLVAGTVNLAMVLVAATNLQGRDNTDSIEG
                     AHAAVRDTLGPTVALFFAIGLLASGLASTSVGAYAGAMIMQGLLRRSYPLLVRRLVTL
                     IPALVILAAGVDPSRALVISQVVLSFGIPFALVPLIRLTSNRAVMGSDVNHRVTTALG
                     WMVAAMIIVLNVGLIYLTVAR"
     gene            1510..2583
                     /locus_tag="VGP27_24635"
     CDS             1510..2583
                     /locus_tag="VGP27_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEV7854522.1"
                     /translation="MLDGRHSYFAYGSNLCVKQMARRCPSAINPQPATLADHDWLINE
                     RGVATVEPFDGSQVHGVVWQLDDHDLATLDSAEGVPVRYRRDRLTVHTDAGPAEAWVY
                     IDYRVDPGPPRPGYLERIIDGALQHGLPHRWIEFLRRWDPSQWPVPKDNSGDGAPQSL
                     SELIADPAVLEESTLRSRFGFMAIHGGGLEQMTDVIAERAADAAGASVYLLRHPENYP
                     HHLPSALYRPEESERLAEFLDHVDVAISLHGYGRLGRSTQLLAGGGNRVLAAHVAAHF
                     DVPGYHVITDLDAIPRELRGLHPDNPVNRVRRGGTQLELSPRLRGISPRSAPADEDGL
                     SPPTSAFIQGLVAAAHSWELAPA"
     gene            2653..3411
                     /locus_tag="VGP27_24640"
     CDS             2653..3411
                     /locus_tag="VGP27_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEV7854523.1"
                     /translation="MKQSDWRADVPALYRTRITHLRRAPVHHYFEHRGYSWYVDVDAL
                     PELPGWLRSFARFEARDHFDGEPTDTLRQRIDALLVSRGIEPPGGTVTALLQARVLGY
                     VYNPLSLYWCHDADGVVRHVLAEVHNPRGDRHVYLLPPDSGEPTIVKKRLYASPFNGQ
                     GGHYLVRAPRPDGDLDVTISLHRDNEPAFVVTMRGNRRQASVAQILRLQLVAPVAPLM
                     VKFGMWIQGVTLRLRRVPVLPESIVTERQKVSQL"
     gene            3408..3992
                     /locus_tag="VGP27_24645"
     CDS             3408..3992
                     /locus_tag="VGP27_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7854524.1"
                     /translation="MTAAATPVSRLLLVTTDLDALLRQVAQRDADAFATFYDLTRARV
                     FGLVVRVLRDPGYSEETTQDVYLQVWRSAANYDPAAGSPLAWLMTVAHRRAVDRVRAE
                     QAATQRESRYGAANVDPPADHVAESVIHLDERRQVTDCLGSLTDVQRECIQLAYYDGL
                     TYVQVSERLSANLATIKSRMRDAIRGLRRCLGLA"
     gene            3989..4705
                     /locus_tag="VGP27_24650"
     CDS             3989..4705
                     /locus_tag="VGP27_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV7854525.1"
                     /translation="MTHPLTPDLVALATPYALHATTDVERAEIERQIAAAPPEVADEF
                     FEQVRAVHETMAVISAATAVEPPPMMRDRILATIADDAVRQLPSKRANRWRTTILAAA
                     AVLAIGLGALGVGIALRPAPTTSTAAQVFAAPDVRTVSGPIPSGGTATLVFSHEKDAG
                     VLVMNNVPPPQPGTVYQMWLVGDNGPQSAGTMDAKSVAPSTTAVLPDLGQSKALAFTV
                     EPGGGSVMPTSPPFAELPLV"
     gene            complement(4737..5831)
                     /locus_tag="VGP27_24655"
     CDS             complement(4737..5831)
                     /locus_tag="VGP27_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV7854526.1"
                     /translation="MASHPTIGVEEEFLLVDPDSGEPVARNETVARNAEARGVKLQLE
                     LTSCQVETTTDVVTTSDQLRTELVRLRRIAAEAADADGARLLAVGLPPTVPHEFPITD
                     SPRYRRIAERFGMIAHEQGICGCHVHVAVPDRDAAIRVSNRLRPWLPLLLAVTANSAV
                     YRNTDTGYASWRSILWQRWPSAGPPPLFESADEYDAVVRMLLDSGAMLDDGMVYWDVR
                     PSANFPTIEVRVADVPATVAETVLLAMLIRGCVMTALEAERRGEGLAPLSPYALRAAY
                     WKSARYGLDGHAIDLTESHASRPAADVLDGLVDHIRPALEAVGDYDLVRAELARISEH
                     GNGAMRQRRAWQRRHDIADVLAEAAAATLE"
     gene            6015..6836
                     /locus_tag="VGP27_24660"
     CDS             6015..6836
                     /locus_tag="VGP27_24660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854527.1"
                     /translation="MSKDFRFGVGLRVAKSLSSVQDEARRAEDLGFDVLHLPDHIGAP
                     APFPTLMAAATATKTLRVGTFVLNACFYKPALLARDVQALRDLSGGRFELGLGAGYAK
                     HEFEAAELRYPSAGERIEYLAHITEYMAEHLPDVPVLIAGNGDRLLTVAAQQADIIGL
                     TGGQPITETTNPLAERIEFVRRAAGDRFDDLELNIAVTAMPRDGSGQPDLSITRRALP
                     HMSDDELLATPAVLSGSTQQIADTIRGYRDTYGVSYVIVQDKHAEAFSKVIAELR"
     gene            complement(7118..7309)
                     /locus_tag="VGP27_24665"
     CDS             complement(7118..7309)
                     /locus_tag="VGP27_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV7854528.1"
                     /translation="MSAIRKAKIEFDRIAGRAKERIGEATGNARLREEGAVDQVRAGA
                     RKTGERVKDVVRGRRRRRY"
     gene            7375..7450
                     /locus_tag="VGP27_24670"
     tRNA            7375..7450
                     /locus_tag="VGP27_24670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7408..7410,aa:Arg,seq:cct)
     gene            complement(7626..8051)
                     /locus_tag="VGP27_24675"
     CDS             complement(7626..8051)
                     /locus_tag="VGP27_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854529.1"
                     /translation="MTTFALHRDSVEFFVDCMHGGADKDALSGILAEDVVMYGPLGDE
                     PLTGREAVLGAMRGVGALAADLTYKEVLSGETHHAAYFRLQIEDAVVNGMDYILLDAD
                     GKIAEVTIWWRPLPSGVQMQRHLAGLLGMQPWELLTDME"
     gene            complement(8346..9080)
                     /locus_tag="VGP27_24680"
     CDS             complement(8346..9080)
                     /locus_tag="VGP27_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854530.1"
                     /translation="MGKLDGKVAVITGATSGMALAGAKLFVEQGAHVFISGRRKDALD
                     QAVELIGRNVTGVQGDSADLDDLDRLFDTVKQEKGAIDVLWASAGTGKQSRLGEITEE
                     DFDAAFWLNARGTLFTVQKALPLFNDGGSIFMTGSNASLRGYPDWSVYAGSKAVLPAY
                     ARVWVSELRDRKIRVNVLTPGQVASPMLAEVMDEEMKAQFESVIPRREMGHPEEIASA
                     ALFLASDDSSYVNGMELVVDGGTTVI"
     gene            9207..>9776
                     /locus_tag="VGP27_24685"
     CDS             9207..>9776
                     /locus_tag="VGP27_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854531.1"
                     /translation="MTELEKGPRGLRRGRGARERILSASRQLFRDQGINCTGMDQLCA
                     VAQVSKRTFYQHFTGKDELIAEHLRRFDPDLLPEVFDRTDLTPRERLLAAFDIHAPLC
                     PFIAAAVEIHDAGHPARVHARDYKKAFAARLIETAREAGAAQPEQLGEQLALLLDGAS
                     ARNRVLNSDTFATAAAIAAVLIDNASPTAA"
BASE COUNT         1581 a         3344 c         3130 g         1721 t
ORIGIN      
        1 atggcggcga ttgccgtcgc acccgtcgcg gccgcagcac cccaatgcac gaacaccggc
       61 ccgaacacga cgcagtgtga gaccaacggc agcgctcaga tcgtgacgtc gccgccggcg
      121 aacaacaact acggctattg gggctacccg atcatcggct tcggcggttg gggtttcggc
      181 atctgagccg ttctggttgg cgtagatctt ttcgggtaac ctaacttttc tattcgacag
      241 cccggataga aggtggggtc ttgctgcgcg acgacaagac gcggatgcgt ccgagctggc
      301 tgctgctcgg cccggcgttc gtcgccgcta tcgcctacgt cgatcccggc aacgtcgcgg
      361 ccaacatcag tgccggtgcg cagtacggct tcctgctggt gtgggtcatc ttcctggcca
      421 acgccatggc cgggctcgtg cagtacctgt cggccaagct cgggctcgtc agcggacgca
      481 ccctgccgga ggccgtcgcc gaccacagcc gcgcctcgac caggatcgcc ttctggctgc
      541 aggccgaaac ggtcgcgatg gcgaccgatc tcgccgaggt cgttggcggc gccatcgcac
      601 tgaacctgct gttcgatctg ccgctgttga taggcggcgt catcaccggc gcggtgtcgc
      661 tgttgctact cgcgatccag aaccgccgcg gccaacgggt gttcgagcgg gtgatcagtg
      721 gcctgctgct gatcatcgca atcggattca cgaccagcct gttcgtcgag cctccgccgc
      781 tcgcggatgc cgccgaaggg ctggtgccgc gcttcgccgg tgccgagagc gtgctgctgg
      841 ccaccgcgat gctgggcgca acggtcatgc cgcacgccgt ctatctgcat tccggtctgg
      901 cccgcgaccg gcacggccat cccgaacccg gcgctcggcg cacctggctg ctccgggtga
      961 cccgtatcga cgtcggcctg gcgatgctgg tggcgggcac cgtcaacctg gccatggtgc
     1021 tggtcgccgc gaccaatctg cagggccgcg acaacaccga ctccatcgag ggcgcccacg
     1081 cggcggtgcg cgacacgctc gggccgaccg tcgcgctgtt cttcgccatc ggattgctcg
     1141 cctccggtct cgcgtcgaca tccgtcgggg cctatgccgg ggccatgatc atgcagggcc
     1201 tgctgcgccg ctcctaccca ctgctggtgc gtcgcctcgt gacgctgatc ccggcgctgg
     1261 tcatcctggc ggccggcgtc gaccccagcc gggccctggt gatctcgcag gtggtgctgt
     1321 ccttcgggat accgttcgcg ctcgttccgc tgatacgtct gaccagcaat cgagccgtga
     1381 tgggcagcga cgtcaaccac cgggtcacca ccgccctcgg gtggatggtg gccgcgatga
     1441 tcatcgtgct caacgtgggg ctgatctacc tgaccgtggc gcggtagccg ctgcgataaa
     1501 ctggaacgaa tgcttgacgg acgccacagc tacttcgcgt acgggtcgaa cctgtgcgtg
     1561 aagcaaatgg cgcgacgttg ccccagcgcc atcaaccccc aaccggcgac actggccgac
     1621 cacgactggc tgatcaacga gcgaggcgtc gccaccgtcg aacccttcga cggctcgcag
     1681 gtgcacggcg tggtgtggca actcgacgac cacgacctcg cgacactgga cagcgccgaa
     1741 ggagtcccgg tccgctaccg ccgcgaccgg ctcacggtgc acaccgacgc ggggccggca
     1801 gaagcctggg tgtacatcga ctatcgcgtc gatcccggac cgccgcggcc cggatatctg
     1861 gaacgcatca tcgacggcgc actgcagcac ggattgccgc accgctggat cgagttcctc
     1921 cgccgatggg atccgtcgca atggccggtg ccgaaggaca actcgggtga tggtgcgccg
     1981 caatcgcttt cagagctgat cgccgatccc gctgtgctcg aggagagcac gctgcggtcg
     2041 cggttcggtt tcatggcgat ccacggcggc gggctggagc agatgaccga cgtcatcgcc
     2101 gaacgggccg cggacgcggc gggcgcgtcg gtgtacctgc tgcgccatcc ggagaactat
     2161 ccgcatcatc tgccgtcggc gctgtaccgg cccgaggagt ccgagcggct cgcggagttc
     2221 ctcgaccacg tcgacgtcgc gatctcgctg cacggctacg gccggttggg gcgcagcacc
     2281 cagctgctcg ccggcggtgg caatcgggtg ctcgccgcac atgtggccgc gcacttcgac
     2341 gtgcccggct accacgtcat caccgacctc gatgcgatcc cgcgcgagct tcggggcctg
     2401 catccggaca atccggtcaa tcgcgtccgt cgcggcggca cccaactcga actctcgccg
     2461 cggctgcgcg gcatcagccc gcgaagcgca ccagcggacg aagacgggct gtcgccgccc
     2521 acgtcggcgt ttatccaggg cctcgtcgcc gccgctcaca gctgggaact ggccccggct
     2581 tagtgccgcg gcacgtagcg cgcatcacag gtcacaccca atccggcatt gcggccgctc
     2641 cgaatacccg gtgtgaaaca aagcgactgg cgcgccgatg tcccggcgct ctatcgaacc
     2701 cgaatcaccc acttgcgtcg ggccccggtt caccactact tcgaacatcg cgggtacagc
     2761 tggtacgtcg acgttgacgc gctgcccgag ctgccaggct ggctgcggtc gttcgcccgt
     2821 ttcgaggccc gcgaccactt cgacggcgaa ccgaccgaca cgttgcgcca acgaatcgac
     2881 gccctgctgg tatcccgcgg gatcgagccg cccggcggca ccgtcaccgc cctgctccag
     2941 gcccgtgtgc tcggctacgt ctacaacccg ctgagcctgt actggtgcca cgacgccgac
     3001 ggtgtagtgc gccacgtgct cgccgaagtg cacaaccccc gcggcgaccg acacgtctat
     3061 ctgctgccgc ccgattccgg cgagccgacg atagtgaaga agcggttgta cgcatcaccg
     3121 ttcaacgggc agggcggcca ctacctcgtc cgcgcaccga gacccgacgg ggatctcgac
     3181 gtgacgatct cgcttcatcg cgacaacgag cccgctttcg tcgtcacgat gcgcggtaat
     3241 cggcggcagg cgagcgtcgc ccagatacta cggctgcagt tggtggctcc agtggctccg
     3301 ctgatggtga agttcggaat gtggatccaa ggagtcacgc tgcgcctgcg tagggtgccg
     3361 gtgctgccgg agtcgatcgt caccgaaaga caaaaggtta gccagctgtg accgcggcgg
     3421 ccacccccgt gtctaggcta ctgctcgtga ccaccgacct tgatgcgtta ctgcggcagg
     3481 tggcacagcg tgacgccgac gccttcgcca ccttctacga cctcacccgc gccagggtgt
     3541 tcggcctggt ggtgcgcgtg ctgcgcgatc ccgggtacag cgaggaaacc acgcaggacg
     3601 tctatttgca ggtatggcgt tcggcggcga actacgaccc agcggcgggt tcgccgctcg
     3661 cgtggctgat gacggtggct caccggcgcg ccgtggaccg cgtgcgtgcc gagcaggcgg
     3721 ccacccagcg tgagtcccgc tacggagccg ccaacgtcga tccgcctgcc gaccacgtcg
     3781 ccgaatcggt cattcacctc gacgagcgcc ggcaggtcac cgactgcctc ggctcactga
     3841 ccgacgtgca gcgggaatgc attcagctcg cctactacga cgggctcacc tacgtgcagg
     3901 tgtccgagcg gctgtcggca aacctggcca ccatcaagtc gcggatgcgc gacgccatcc
     3961 gcggtctacg cagatgtctg gggctcgcat gactcatcca ctcacacccg atctggtcgc
     4021 gctggccacg ccctacgcgt tgcatgccac gactgacgtc gaacgcgccg agatcgaaag
     4081 gcagatcgcc gctgccccgc ccgaggtggc cgacgagttc ttcgaacagg tgcgtgccgt
     4141 gcacgagacg atggcggtca tctccgccgc gacggcggtc gaaccacctc cgatgatgcg
     4201 cgaccggata ctggcgacga ttgccgacga cgccgtgcga caactgccat cgaagcgtgc
     4261 gaaccgttgg cggacaacca ttctggcggc tgcggcggtg ctcgcgatcg ggcttggcgc
     4321 gctgggcgtc gggatcgcgc tgcggcccgc gcccacgacg tcgacggccg cgcaggtgtt
     4381 cgcggcaccg gacgtgcgga ccgtatccgg tcccattccg agcggtggca ccgccacgct
     4441 ggtgttctcc cacgagaagg acgcgggcgt gctggtgatg aacaacgtcc cgccgccgca
     4501 gcctggcacc gtgtatcaga tgtggttggt cggcgataac ggcccgcagt cggcgggcac
     4561 catggacgcc aagtccgtcg cgccgtcgac gaccgctgtg ctgccggatc tcggccagtc
     4621 gaaggcgctg gccttcaccg ttgaacccgg cggcggatct gtcatgccga ccagccctcc
     4681 gttcgccgaa ctgccgctgg tctgacgggc ttcttctcct ggtcctcgcg tgcgcgctac
     4741 tccaaggttg ccgcggcggc ctcggcaagg acgtcggcga tgtcgtgcct gcgctgccat
     4801 gcgcgccgct gccgcatcgc gccgttgccg tgctcggaga tgcgcgcgag ttcggcgcgc
     4861 accaggtcgt agtcgccgac cgcctccagc gccggccgga tgtggtcgac gagtccgtcg
     4921 aggacatccg cagcaggtcg cgaggcgtgg ctttcggtca ggtcgatggc gtgaccgtcg
     4981 aggccgtagc gcgccgactt ccaataggcg gcccggagcg catacgggga aagcggcgcg
     5041 agaccttcgc cgcgccgttc ggcttcgagc gcggtcatca cacagcccct gatcaacatc
     5101 gcaagcagaa ccgtctcggc gaccgtggcg ggcacgtcgg cgacgcgaac ctcgatcgtc
     5161 gggaagttcg cggacggccg gacgtcccaa taaaccatgc cgtcgtcgag cattgcgccg
     5221 ctgtccaaaa gcatccgaac gacagcgtcg tactcgtcgg cggactcgaa aagcggcggc
     5281 ggtccggcgc tcggccatcg ttgccacagg atgctgcgcc agctggcata tccggtgtcg
     5341 gtattgcggt acaccgcaga gttggcggtc accgccaaca gcagcggcag ccatggccgc
     5401 agccgattgc tcacccggat ggcggcgtca cggtcgggaa cggcgacgtg cacatggcat
     5461 ccgcagatgc cctgctcgtg ggcgatcatt ccgaaccgct cagcgatccg ccggtagcgc
     5521 ggactgtcgg tgatcgggaa ttcgtgcggc acggtcggcg gcaacccgac ggcgagcagc
     5581 cgcgcaccgt cggcgtcggc ggcctcggcg gcgatgcgcc gtaaccgaac cagctcggtg
     5641 cgcaactggt cgctggtggt caccacgtcg gtcgtcgtct cgacctgaca gctggtcaac
     5701 tcgagctgca gcttcacacc gcgggcctcg gcgttacggg cgacggtctc gttgcgggcg
     5761 accggttcgc cggaatcggg gtcgacgagc aggaactcct cttcgacacc gatggtggga
     5821 tgactggcca tggctgctcg ctcaaaagat agatccggcc cgtcggtgat tcacgacggg
     5881 ccggatcgcg cggtggctcc tcttgcggtg gtactccgcg ggccgcgaag tctggcggtt
     5941 cagaattgcc gaaccgctcg tggcccaaac atcagaattc tctgaaggtg attccgcggg
     6001 cttacgatcg cggagtgagc aaggatttcc ggttcggcgt cggcctgcgc gtcgccaagt
     6061 ccctgtcgtc ggtacaggac gaggcgcgcc gggccgagga tctcggcttc gacgttttgc
     6121 acctgcccga tcacatcggg gcgcccgcgc cgttcccgac gttgatggcg gcggcaacgg
     6181 cgacgaagac gttgcgggtc ggcaccttcg tactgaacgc ctgcttttac aaaccggcct
     6241 tgctggcgcg cgacgtgcag gcgctgcgcg atctttccgg cggccgtttc gagctcggct
     6301 tgggcgcggg gtatgcaaag catgagttcg aggccgccga gctgcgctac cccagcgccg
     6361 gcgagcgcat cgagtacctc gcgcacatca ccgagtacat ggccgagcac cttcccgatg
     6421 ttccggtgct gatcgccggg aacggcgacc gactgctgac ggtggcagca cagcaggccg
     6481 acatcatcgg gctgaccggc ggtcagccga tcaccgagac gacgaacccg ctcgccgagc
     6541 ggatcgaatt cgtccgccga gccgcgggtg accgattcga cgacctcgaa ctgaacatcg
     6601 cagtgacggc aatgccgcgc gacgggtccg ggcaacccga cctgtcgatc acccgccgag
     6661 cactgccgca tatgtcggac gatgaactgc tggcgacgcc ggctgtgctg tcggggtcga
     6721 cccagcagat cgccgacacc attcgcgggt atcgcgacac ctatggcgtg agctatgtga
     6781 tcgttcagga caagcacgcc gaagcgtttt ccaaggtgat cgccgaactg cgctagttgg
     6841 acaacagttg cgcgtttgaa ctgcctccct gcgggaactg ttagtcccta tgcagatcga
     6901 gcgggcggcg cggcggtgat cgagtgatgc gctcgacgct ggtacacatc cgagtggtgt
     6961 tcacaatgtg ctggcactcc ccgaacacct tcgcctgttg gctgcgcgcc gcttatccgc
     7021 aatggtcccc gcggccgggc catgtgccgc tcattgcgct gtgcgggcgg gaggggtcgc
     7081 tgatcgagca gcgtccccag tcggcgcggc cggcgagcta gtacctgcgg cgtctgcggc
     7141 cgcgcaccac atccttcacc cgttcacccg ttttgcgcgc cccggcccga acttggtcca
     7201 cagcaccttc ttcgcgcagt cgtgcattgc ccgtcgcttc accgatccgt tccttcgccc
     7261 ggcctgcgat gcggtcgaac tcgattttgg ccttccgtat tgcactcatg ggtgcagtgt
     7321 ttccgctgcg cagcgagtag aaacctggcg ccacggtaag ctcccagcgt ccccgccctc
     7381 gtagctcagg ggatagagca cggctctcct aaagccggtg tcgcaggttc gaatcctgcc
     7441 gggggcacca agtgcgttgt attacctcgg ttggacattt cgtcttcggt tgatgggcga
     7501 cagtgtttcg gttgatgatt gacagtggct tcggctgatc cttgacactc ccttgatgag
     7561 ggagttaagc gacagcggcc cgtccggtgc ggtgaagact ttgccctcgg gatcgggctt
     7621 caccgttact ccatatcggt gagaagttcc cagggctgca tgccgaggag accagcaagg
     7681 tgccgctgca tctggacgcc ggacggcagc gggcgccacc atatggtcac ctcggcgatc
     7741 ttgccgtccg cgtcgagcag gatgtagtcc atcccgttga ccacggcgtc ttcgatctgc
     7801 agccgaaagt acgcggcgtg gtgcgtctct ccgctgagaa cctccttgta ggtgaggtcg
     7861 gccgccaacg cgccgacccc tcgcatggct cccaggactg cctcgcggcc ggtgagtggc
     7921 tcatcgccga gcgggccgta catcaccacg tcctcggcga gaatgccgga aagagcgtct
     7981 ttgtctgcgc cgccgtgcat gcagtcgacg aagaattcca cggagtcgcg gtgcagggcg
     8041 aacgtcgtca tggttactcc ttatgtcctt tgttgcttgt cgtgtgtgag acccggtcgg
     8101 tgcgcccacc cgttcgagtc ggtgtgccag gtcatttaag gtgagatgaa ctcgccgact
     8161 gagaatcaat ttcatcgatt cctgctagcg ccatattgag tggttgtggt ggacgactgc
     8221 gctgagtggc cgcagggtcg cggcaacctc gctgcggggc cggatccgcg agttcatcag
     8281 gccgggatgg tcgccccggt ccggggcgct acgtttttgt cggcatacat gccgtcgtcg
     8341 ctcattcaga tcactgtggt gccgccgtcg accaccaact ccatgccatt gacatagctc
     8401 gagtcgtcgg aggcgaggaa caacgcggcc gacgcgatct cctcggggtg gcccatctct
     8461 cgccgcggga tcacggactc gaactgcgcc ttcatctcct cgtccatcac ctccgccaac
     8521 atcggcgacg cgacctggcc gggggtcagc acgttcaccc ggatcttcct gtccctcaac
     8581 tcggacaccc acactcgtgc gtaggcgggc agcacggcct tgcttcccgc gtacacactc
     8641 caatcggggt agccccggag cgaagcgttc gacccggtca tgaagatcga gccgccatcg
     8701 ttgaacagcg gcagcgcctt ctggaccgtg aacagcgtgc cgcgcgcgtt caaccagaag
     8761 gcggcatcga agtcctcctc ggtgatctcg ccgagcctgc tctgcttgcc cgtcccggca
     8821 cttgcccaca acacgtcgat cgcgcccttt tcctgcttga ccgtgtcgaa caaacggtcc
     8881 agatcgtcga ggtcggccga atcgccctgc acgccagtca cgttccgccc gatcagttcg
     8941 acggcctggt ccagcgcgtc cttccgtcgg cccgagatga agacgtgagc tccttgttca
     9001 acgaacaact tggcaccggc cagcgccata ccacttgtcg cgccggtgat caccgcgacc
     9061 ttgccatcga gctttcccac gatcactcca atcattcgat agtgccgatg ctatgtacac
     9121 cgatcagcgt gcttaacgta tcggatagcg atgcggagtg caagctatgc acaccggtcg
     9181 gtacccaacc gggctacgct gggtgtatga cggagttaga gaaggggccg cgaggcctgc
     9241 gccgcggcag gggcgcacga gagcgcatcc tcagtgcttc acgacagctg ttccgcgatc
     9301 aaggcatcaa ctgcaccggc atggaccagc tctgcgcggt ggcccaggtg tccaagcgca
     9361 cgttttacca gcacttcacc ggcaaggacg agctgatcgc cgaacaccta cgccgtttcg
     9421 atcccgacct tctgcccgaa gtgttcgacc gcaccgacct gacaccccgc gaacggctcc
     9481 tcgccgcctt cgacattcac gcgcctctgt gcccgttcat tgcggcggcc gtcgaaatcc
     9541 acgacgcggg ccacccggca cgtgtgcacg cccgcgatta caagaaggct ttcgccgctc
     9601 ggctcatcga aaccgcgcgt gaggccggcg ccgcccaacc cgaacagctc ggcgaacaac
     9661 tggcgctgct cttggatggc gcttcggccc gcaaccgagt actcaactcc gacaccttcg
     9721 ccaccgccgc cgccatcgcc gccgtcctca tcgacaacgc ctcccccacg gcggcg
//
LOCUS       DASYLX010001053         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2595889,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001053 DASYLX010000000
VERSION     DASYLX010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595889"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGP27_24690"
     CDS             <1..286
                     /locus_tag="VGP27_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HEV7854532.1"
                     /translation="DAAITAWSKFDGAVAEARLFPLAQFMGDVYAGALLIEQAAWERA
                     ENGTDRKALVARLYAQRQLADRGPLRGLDADGDEAIERFDELVAGALAVT"
     gene            complement(356..1954)
                     /locus_tag="VGP27_24695"
     CDS             complement(356..1954)
                     /locus_tag="VGP27_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854533.1"
                     /translation="MADREGLSLGRLLLTLDRTMVTLVEAPRGLDMPVGSVALVDADD
                     IRLGLAVGAGAADLFFLLGVSDAEAVGWIAQQFQGTGRVPAAIFVKEPSHAAVKQAVA
                     LGTAVVAVEPRARWELLYKLVNHAFEHHGDRGDPLHDSGTDLFGLAQSMAERTHGMIS
                     IEDDQSHVLAYSASNEEADDLRRLTILGRAGPPEHLEWIGQWGIFDALQASSDVVRVA
                     ERPELGLRPRLAVAIHLPATDVRRPPRFAGTIWLQQGSAPLADDVEEVLRGGAVLAAR
                     IMSRLASAPSTHTVLVQDLLGLGLGEESADVAAIARELGIDADGRAALVGFRGEGTAA
                     PSNVIALSASAFRTDAQLASTGERVYVLLPKIGATSSLTSWVRGMVAALRRELGLTMR
                     AVIAAPLAGLAGAAQARTEIDRVFDSADRHPGAIGQVTSLGEAHTTVLLDEIVAHVAG
                     RQRLVDPRVRELRERDPMLADTLAAYLDGFGDIAEVARRLHVHPNTVRYRIRRIEKLL
                     STSLDDPDDRLVLALGLRATERSP"
     gene            2049..>2549
                     /locus_tag="VGP27_24700"
     CDS             2049..>2549
                     /locus_tag="VGP27_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854534.1"
                     /translation="MDAVTEVPTPANEPVHDYAPSSPERARLVTALAELDDDPIDLPH
                     VIGGVHRMGGGERIEVVQPHRHSAKLGTLTNAEHSDATAAIDAATAAKEGWANTPFDE
                     RAAVFLRAADLLAGPWRAKLCAATMLGQSKSAYQAEIDAACELIDFWRFNVGFASEIM
                     AEQPINT"
BASE COUNT          437 a          889 c          848 g          375 t
ORIGIN      
        1 cgatgcggcg atcacggcgt ggagcaagtt cgacggcgcc gtcgcagaag ccaggctgtt
       61 tcctctcgcg caattcatgg gcgatgtcta cgccggtgcg cttttgatcg agcaggcagc
      121 ctgggaacga gccgaaaacg gaactgaccg aaaggctctt gtggcccggc tgtatgcgca
      181 gcggcagttg gccgaccgcg gcccgctgcg cgggctcgac gccgacggtg acgaggccat
      241 cgaacgattc gatgagctgg tggccggggc gctcgcggtg acgtaatacc cgcgagcaga
      301 cgcaaaatcg cacaatttcg cggcgtttta tgcgattttg cgtctgctcg ccgaactagg
      361 gtgagcgctc ggtcgcccgc agaccgaggg cgagcacgag ccggtcgtcg gggtcgtcca
      421 acgacgtcga cagcagcttc tcgatacgac ggatcctgta gcgcaccgta ttcggatgta
      481 catgcagccg ccgcgccacc tcggcgatgt cgccgaaccc gtccagatag gcggccaacg
      541 tgtcggcgag catcgggtcc cgctcacgca attcgcggac gcgcggatcg acgagccgct
      601 gcctgccggc cacatgcgcg acgatctcgt cgagcagcac cgtggtgtgc gcctcgccca
      661 atgacgtcac ctgaccgatc gcgccggggt gtcggtcggc gctgtcgaac acccgatcaa
      721 tctcagtgcg ggcctgggcc gcaccggcca aaccggccag cggcgcggcg atcaccgccc
      781 gcatcgtgag gcccagctcg cggcgcagtg cggcgaccat cccccgcacc cacgacgtca
      841 gcgacgacgt cgcaccgatc ttcggcagta gcacatatac gcgctcaccc gtcgacgcca
      901 actgggcgtc ggtgcgaaaa gcgctcgcgc tcaacgcgat cacgttggac ggagccgcgg
      961 ttccttcgcc gcggaagccg acgagggcgg cgcggccgtc ggcgtcgatg ccgagttcac
     1021 gggcgatcgc tgcgacgtcg gcggactcct cgccgagccc gagcccgagg aggtcctgca
     1081 ccagcacggt gtgcgtcgac ggcgccgacg ccagccgcga catgatgcgg gccgccagca
     1141 cggctccgcc gcgcaacact tcttccacgt cgtcggccag cggcgctgaa ccctgctgca
     1201 accagatcgt tcccgcgaag cggggcggac ggcggacgtc cgtggccgga aggtgaatgg
     1261 caacggcgag ccgcgggcgg aggccgagtt cggggcgttc ggcgacgcga accacgtcgc
     1321 tgctggcctg taaggcgtcg aaaatgcccc actggccgat ccattccagg tgttcgggcg
     1381 gaccggcgcg ccccagaatg gtcagccggc gcagatcgtc ggcctcctcg ttggacgccg
     1441 agtaggccag cacatgcgac tggtcgtcct cgatgctgat catcccgtgg gtgcgctcgg
     1501 ccatggactg cgccagcccg aacaggtcgg tgccggagtc gtgcagcggg tcgccgcggt
     1561 cgccgtggtg ctcgaacgcg tggttcacca atttgtagag cagttcccac cgggcgcgcg
     1621 gttcgacggc gaccaccgcg gtgccgagcg ctacggcctg tttcaccgcg gcgtgcgacg
     1681 gctccttgac gaagatggcc gcgggaaccc tgccggtgcc ctggaactgc tgcgcgatcc
     1741 agccgaccgc ctccgcgtcg gaaactccga gcaggaagaa cagatccgcg gcacctgctc
     1801 cgacggccaa gcccagccgg atgtcgtccg cgtcgacgag tgccaccgac cccaccggca
     1861 tatcaagccc tcgaggcgcc tcgaccagcg tcaccatcgt gcggtccaac gtgagcagca
     1921 gccggccgag cgacagtccc tcccgatccg ccatcgaacg ctcctttgtc cgatccgact
     1981 ataagccgcc gacggtattg tcggatcgac catcggttgg aaagggggcg aaagggcatc
     2041 cttactgcat ggatgccgtc accgaggtac cgacaccggc caacgagccg gttcacgact
     2101 acgcaccgag cagtcccgaa cgcgcgcgac tcgtcactgc gctcgccgaa ctggacgacg
     2161 acccgatcga tctgccccac gtcatcggcg gcgtgcaccg gatgggcggc ggtgaacgca
     2221 tcgaggtggt gcaaccgcat cgccattccg ccaagctggg cacgctcacc aacgccgaac
     2281 actccgacgc gaccgccgcc atcgacgcgg ccacggcagc caaggagggt tgggcgaaca
     2341 ccccgttcga cgagcgcgcc gccgtcttcc tgcgcgccgc cgacctgctg gccgggccgt
     2401 ggcgtgcgaa gctctgtgcg gcaacgatgc tcggccagtc gaagtcggcc tatcaggctg
     2461 agatcgacgc ggcctgcgag ctgatcgact tctggcggtt caacgtcggc ttcgccagcg
     2521 agatcatggc cgaacagccg atcaacacc
//
LOCUS       DASYLX010001054         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1499858,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001054 DASYLX010000000
VERSION     DASYLX010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499858"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGP27_24705"
     CDS             <1..394
                     /locus_tag="VGP27_24705"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEV7854535.1"
                     /translation="LGVDPKTLDRTIHVHVPAGAVPKDGPSAGVTMVTALVSMATGRQ
                     VRSDVGMTGEVTLNGRVLPIGGVKQKLLAAQRAGLSTVFIPQRNEPDLDDVPAEVLEA
                     LEVRPMTDVADIVARALEPVGAEATVAA"
     gene            471..803
                     /locus_tag="VGP27_24710"
     CDS             471..803
                     /locus_tag="VGP27_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV7854536.1"
                     /translation="MAYDEDLADRIRELLGAHKGVEEKRMFGGLAFLINGNMSVAASG
                     RGGLMVRVPPTETDSLLSREHVEPMVMAGRETRGWLRVSADGVKTKRQLRSWVTRGVD
                     YAKSLPPK"
     gene            846..1487
                     /locus_tag="VGP27_24715"
     CDS             846..1487
                     /locus_tag="VGP27_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEV7854537.1"
                     /translation="MARSTHKQLVERLMKVAGTTYAEEAGITLRDKPMPLFQLLVLCM
                     LASKPIDATIAVQAARELFRAGLKTPKAVLAADRDTMIAAFGEAHYVRYDESSATRLA
                     DIAAAVRDDYAGDLRRLAAAGKCDVTAVKRLLKQFKGIGDTGADIFLREVQDVWTWAR
                     PYFDQRAITAARAIGLPADPAALSALAPRNTAKLAAALVRVSLDDDKRAELVA"
     gene            1484..>2012
                     /locus_tag="VGP27_24720"
     CDS             1484..>2012
                     /locus_tag="VGP27_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV7854538.1"
                     /translation="MIRIGTSGWSYDHWTGVLYPPGLPPAKRLARYVEEFDTVELNAS
                     FYRWPRDAAFAGWRQRLPDGFTMAVKAHRGLTHFRRLTSPEPWIERFERCWTALGDRA
                     EALLVQLHPELRRDDARLDHFLGLMPRSIPVAMELRHPSWNDPAVFSLLERHGAAYVV
                     MSGAKLDCVPVATSDL"
BASE COUNT          339 a          689 c          667 g          317 t
ORIGIN      
        1 gttgggcgtc gacccgaaga cactggaccg gacgatccac gtgcacgtgc ctgcgggcgc
       61 ggtgcccaag gacggcccgt ccgcaggcgt caccatggtc accgccctgg tgtcgatggc
      121 caccggacgt caggtccgct ccgacgtggg tatgaccggt gaggtcacgc tcaacggtcg
      181 ggtgctgccg atcggtgggg tcaagcagaa gctgcttgcc gcccaacggg ccggtctgtc
      241 aacggtcttc atcccgcagc gcaacgagcc cgacctggac gacgtcccgg ccgaggtgct
      301 ggaggcgctc gaggttcggc cgatgaccga tgtcgccgac atcgtcgcgc gggcactcga
      361 acccgtcggc gcggaagcca ccgtcgccgc ctgatcgcaa gattgcactc agggtcgtga
      421 tcacagaaca ggtcacgacc ctgagtgcat tttcggtgat acttcatcgc atggcgtatg
      481 acgaggacct ggccgaccgt atccgcgaac tcctcggcgc ccacaaaggc gtcgaggaga
      541 agcggatgtt cggcggtctg gcgtttctga tcaacggcaa catgtcggtg gccgcaagcg
      601 gccgcggcgg cctgatggtt cgcgtgccgc ccaccgaaac cgactcgctg ctttcgcgcg
      661 agcacgtcga accgatggtg atggcgggcc gggagacccg cggctggctt cgggtctccg
      721 ccgacggcgt caagaccaaa cgccaactgc ggtcctgggt cacccgcggc gtcgactacg
      781 ccaaatccct gcccccgaaa tgagcccgac gtttccgcac cattgcgacg ggtaagtcag
      841 gagcaatggc acggtcaacg cacaagcagc tcgtggagcg gctgatgaag gtcgccggca
      901 ccacctacgc cgaagaggcc ggcatcacgc tgcgggacaa accgatgccg ctgttccagt
      961 tgctggtgtt gtgcatgttg gccagcaagc ccatcgacgc gacgatcgcc gtgcaggctg
     1021 cgcgggaact gttccgggcc ggcctgaaaa ccccgaaggc ggtcctggcc gccgaccggg
     1081 acaccatgat cgccgcattc ggagaggcgc actacgtccg ctacgacgag agctcggcga
     1141 cccggttggc cgacatcgcc gccgctgtcc gtgacgacta cgcaggcgac ctgcggcggc
     1201 tggcggccgc aggcaagtgc gatgtcactg cggtgaagcg actcttgaag cagttcaagg
     1261 gaatcggcga caccggcgcc gacatcttcc tccgcgaagt gcaggacgtg tggacctggg
     1321 cgcggccgta cttcgaccag cgcgccatca ccgcggcacg tgccatcggg ctgcccgccg
     1381 atcccgccgc gttgagcgca cttgcaccac ggaacaccgc gaagctcgcc gcggcactcg
     1441 tccgagtctc cctcgacgac gacaaacgcg ccgaactcgt cgcgtgatcc ggatcggcac
     1501 ctccggctgg tcgtatgacc actggaccgg cgtgctgtat ccccccggcc tgccgccggc
     1561 caaacggttg gcccgctacg tcgaggagtt cgacaccgtc gagctcaacg ccagcttcta
     1621 ccggtggccg cgggacgccg cctttgccgg atggcggcag cgcctgcccg acggcttcac
     1681 catggcggtg aaggcccatc gcgggctgac ccacttccgc aggctgacat cacccgagcc
     1741 gtggatcgag cgcttcgagc ggtgctggac ggccctcggc gaccgcgcgg aggcgttgct
     1801 cgttcagctg caccccgaac tgcggcgcga cgacgcccgg ctagaccact ttctcggcct
     1861 gatgccgcga tcgatcccgg ttgcgatgga gctgcgacat ccgtcgtgga acgacccggc
     1921 cgtgttctcc ctgctggaac gacacggtgc ggcgtatgtg gtgatgagtg gggccaaact
     1981 cgactgcgtt ccggttgcca ccagtgacct cg
//
LOCUS       DASYLX010001055         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1390011,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001055 DASYLX010000000
VERSION     DASYLX010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390011"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGP27_24725"
     CDS             <1..314
                     /locus_tag="VGP27_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron reductase"
                     /protein_id="HEV7854539.1"
                     /translation="RDGIVRLPSEAALTTWVAHRSHRALEPLFASIDDVSGGAISQAS
                     MWHIVGAAVVGAATHVPLLARSSEVTSMRRAQAVLDSLVGFGLPVRGGSRLRGGKGLL
                     N"
     gene            complement(432..809)
                     /locus_tag="VGP27_24730"
     CDS             complement(432..809)
                     /locus_tag="VGP27_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7854540.1"
                     /translation="MGKSVKDLVAAANAVVPRITPDEARQKLEAGAVVVDVRDAAEVA
                     KSGKVAGAVHIPRGMVEFRADPETPYYDANFDKDKAVILYCASGGRAALSGQALKEMG
                     YAEVYNLGGFNDWAESGGAVEKA"
     gene            870..1274
                     /locus_tag="VGP27_24735"
     CDS             870..1274
                     /locus_tag="VGP27_24735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7854541.1"
                     /translation="MSSVPIDDLGKGFDTELGLKYIEVTPDGGSAALVITDKLLQPWG
                     IVHGGVYCSIIESLASVSGAVWLSENGGGTVVGVNNNTDFLRAITSGAVTAVSTPIHR
                     GRRQQLWLITITDENDRVVARGQVRLQNIPQE"
     gene            1329..1631
                     /locus_tag="VGP27_24740"
     CDS             1329..1631
                     /locus_tag="VGP27_24740"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEV7854542.1"
                     /translation="MRLTPHEQDRLLISYAADLARRRRERGLRLNHPEAVALLTDHLL
                     EGARDGRTVAELMVTGREVLGREDVMEGVPEMLHDVQVEATFPDGTKLVTVHHPIP"
     gene            1628..1942
                     /locus_tag="VGP27_24745"
     CDS             1628..1942
                     /locus_tag="VGP27_24745"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEV7854543.1"
                     /translation="MIPGEIVYGDGDITINPGAPRLPMDVVNTGDRPVQVGSHVHLPQ
                     ANAALHFDRSAAHGHRLDIPAGTAVRFEPGVIQHVSLVPLSGTREVHGLSLDPPGRLD
                     AS"
     gene            1939..3660
                     /locus_tag="VGP27_24750"
     CDS             1939..3660
                     /locus_tag="VGP27_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEV7854544.1"
                     /translation="MTDLSRARYAALFGPTTGDRIRLADTDLFVEITEDRSGGPGQAG
                     DEAVFGGGKVLRESMGQGRATRADGAPDTVITGVVIIDYWAIVKADIGIRDGRIVAIG
                     KAGNPDTMTGVHPDLVVGPSTEIIAGNGRIVTAGAIDCHVHLICPQIMEEALGGGITT
                     IVAGGTGPAEGSKATTVTPGAWHLARILEALDGWPLNVALLGKGNTVSTEAMWEQLRG
                     GAAGFKLHEDWGTTPAAIDACLKVADASGVQVNIHTDTLNEMGFVENTLSAIGGRSIH
                     AYHTEGAGGGHAPDIITVAAESHVLPSSTNPTRPHTVNTLDEHLDMLMVCHHLNPSVP
                     EDLAFAESRIRPSTIAAEDLLHDIGAISMIGSDAQAMGRIGEVVLRTWQTAHVMKRRR
                     GALAGDGAADNNRARRYVAKYTICPAITHGLDAEIGSVEAGKLADLVLWEPAFFGVRP
                     HAVIKGGMIAWAAMGDANASIPTPQPVLPRPMFGAAPAAAAATSVHFVSPQAIEDGLA
                     DRLAVNRRLVPVGDVRKTGKADMPLNDAMPRIEVEPDTFTVRIDGEVWQEQPAAELPM
                     AQRYFLF"
     gene            3660..4295
                     /locus_tag="VGP27_24755"
     CDS             3660..4295
                     /locus_tag="VGP27_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEV7854545.1"
                     /translation="MTSLSTLLALADSRLPTGGHVHSGGVEEAVTSGLVVDLATLRAF
                     LRRRIATQGLVTASMAAAVHTGSLCFAAADLETDARTPAPAARQASRAQGRGLARLAR
                     RVWPGGEWDALGATPHFAVAAGAVGTASGMTPQHTALSIVYTTMTGSATAAQRLLALD
                     PGDVAALTFELSDMCDRTADLACKELADLSDPLLDVLAQRHIERERPLFVS"
     gene            4314..>4520
                     /locus_tag="VGP27_24760"
     CDS             4314..>4520
                     /locus_tag="VGP27_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7854546.1"
                     /translation="MPPHFIDGEPHRPHPHRPKRARTAGEPLRIGVGGPVGSGKTALV
                     AALCRELRDELSLAVLTNDIYTTED"
BASE COUNT          738 a         1589 c         1497 g          696 t
ORIGIN      
        1 cccgcgacgg catcgtccgg ctgcccagcg aggcggcgct gacgacctgg gtggcccacc
       61 gaagccaccg ggcactcgag ccgttgttcg cctcgatcga cgacgtcagc ggcggagcga
      121 tctcgcaggc gtcgatgtgg cacatcgtcg gcgcggcagt ggtcggcgcc gcgacccacg
      181 ttccgctgct tgccaggtcg agcgaggtga cgagcatgcg ccgcgcacaa gccgtgctcg
      241 actcactcgt cggcttcgga ctcccggtac gcggcgggtc tcggcttcga ggagggaagg
      301 gcttgcttaa ttagggcagc cttgcctatt gtgtcttcag ttggaccgag caggtgtcct
      361 gagggctgca gagacccccg gtccactcca ggcgggtccc gtgccatcgg cgcggggccc
      421 gcccactgct atcaggcctt ctccaccgcg ccgccgctct ctgcccagtc gttgaacccg
      481 cccaggttgt agacctcggc gtagcccatc tctttgagcg cctgtccaga cagcgccgcg
      541 cggccaccgg aggcgcagta caggatgacc gccttgtcct tgtcgaagtt cgcgtcgtag
      601 tagggcgtct cggggtcagc ccggaactcc accatcccgc gcgggatgtg taccgcaccc
      661 gcgaccttgc cgctcttcgc gacttccgcg gcgtcgcgca cgtcgacgac gacggcgcct
      721 gcctcgagtt tctgccgtgc ttcgtcaggc gtgatgcgcg gcacgacggc gttggccgcc
      781 gccaccagat ccttgacact ttttcccatg cccgacggta acgcgaggcc cgctgcgggc
      841 gttgagactt caccgtgtag acactccttg tgtcaagtgt gccaatagac gatctcggta
      901 aggggttcga caccgaactc ggcctcaaat acatcgaggt caccccggac ggcggaagcg
      961 ctgcgctggt catcacggac aaactgctgc agccgtgggg catcgtgcac ggcggcgtct
     1021 actgctcgat catcgagagc ctggcgagcg tctcgggggc tgtctggctg tccgagaacg
     1081 gcggcggcac cgtcgtcggc gtcaacaaca acaccgactt cctgcgcgcc atcacatctg
     1141 gcgcggtgac agcggtgtcg acaccgatac accggggcag gcgccagcag ctctggctga
     1201 tcaccatcac cgacgagaac gaccgcgtcg ttgcacgcgg tcaggtgcgg ttgcagaaca
     1261 tcccgcagga gtagcgcgcg gccggcgtgc gctgcggact tgcgcgaccc gtggcagcat
     1321 cgcccactat gcgtctgacc ccgcacgaac aggaccggct gctgatctct tacgcggccg
     1381 atctcgcgcg gcgccgtcga gaacgcggcc tgcgcctcaa tcacccagaa gctgtcgccc
     1441 tactcaccga ccatctgctc gaaggtgccc gcgacggacg cacggtcgcc gagctgatgg
     1501 tcaccggccg tgaggtgctc ggccgcgaag acgtcatgga gggcgtcccc gagatgctgc
     1561 acgacgtcca ggtggaggcc accttccccg acggcaccaa gctggtcacc gtccaccacc
     1621 cgatcccatg atcccgggcg aaatcgtgta cggcgacggc gatatcacca taaatccggg
     1681 cgcgccgcgc ctcccgatgg atgtggtcaa caccggcgac cgtccggtgc aggtcggtag
     1741 ccacgtccac ctcccgcagg ccaacgcggc gctccacttc gaccgctccg ccgcccacgg
     1801 ccaccggctc gacatcccgg caggcacagc cgtccgtttc gaacccggtg tcatccagca
     1861 tgtttcgctg gtaccgctga gcggcacacg cgaggtgcac gggctcagcc tcgatccacc
     1921 gggccgactg gacgcctcat gaccgacctg tcgagagccc gttacgcggc cctgttcggt
     1981 cccaccacgg gggaccggat ccggttggcc gacaccgacc tgttcgtcga gatcaccgaa
     2041 gaccgcagcg gcggtccggg gcaggcgggc gacgaggcgg tattcggcgg cggcaaggtg
     2101 ctgcgcgaat cgatgggcca gggacgcgcg accagggccg acggtgcccc cgacaccgtc
     2161 atcaccggtg tggtgatcat cgactactgg gcaatcgtca aggccgacat cggcatccgt
     2221 gacggccgta tcgtcgcgat cggcaaggca ggcaacccgg acaccatgac cggcgtacac
     2281 cccgatctgg tcgtcggccc gtcgacggag atcatcgctg gcaacggccg gatcgtgacc
     2341 gcgggtgcca tcgactgcca cgtccacctg atctgtccgc agatcatgga ggaggcgctc
     2401 ggaggcggca tcaccaccat cgtcgccggc gggacgggtc ctgcggaggg cagcaaggcg
     2461 accaccgtca cgcccggcgc ctggcacctg gcccggatcc tcgaggcgct cgacggctgg
     2521 cccctgaacg tcgcactcct cggcaagggc aacacagtca gcaccgaagc gatgtgggag
     2581 caattgcgcg gcggcgcagc gggtttcaag cttcacgaag attggggcac gacgccggcg
     2641 gcgatcgacg cctgcctgaa ggtcgccgat gcctccggcg tgcaggtgaa catccacacc
     2701 gacaccctca acgagatggg cttcgtcgag aacacgctga gtgcgatcgg cggtaggtcc
     2761 atccacgcct accacacgga gggtgcgggc ggcgggcatg cgcccgacat catcacggtc
     2821 gccgccgaat cacacgtcct gccgagttcg accaacccca cccggccaca caccgtcaac
     2881 accctcgacg agcacctcga catgttgatg gtgtgtcacc acttgaaccc gagtgtcccc
     2941 gaggatctcg ccttcgccga gagccggatc aggccgtcga ccatcgccgc ggaggacctg
     3001 ctgcacgaca tcggcgccat ctcgatgatc ggtagtgacg cccaggccat gggccgcatc
     3061 ggcgaggtgg tgttgcgcac ctggcagacc gcgcacgtga tgaagcgtcg tcgcggcgcc
     3121 ctggcgggtg acggcgcggc cgacaacaac agggcccgcc gctacgtcgc gaaatacacc
     3181 atctgcccgg cgatcacgca tgggctcgat gcagaaatcg gttcggtcga ggcgggcaag
     3241 ctcgccgacc tggtgctgtg ggagcccgcc ttcttcggcg tccggccgca cgcggtgatc
     3301 aagggcggca tgatcgcgtg ggcggcgatg ggcgatgcga acgcgtcgat cccgactccg
     3361 cagccggtgt tgccccggcc gatgttcggg gcggcacccg ccgcggctgc ggcgacctcg
     3421 gtgcacttcg tgtcgccgca ggcgatcgag gacgggctgg ccgatcggct ggccgtcaac
     3481 cgccgcctgg tgccggtcgg tgacgtccgc aagaccggca aggcggatat gccgctcaac
     3541 gacgccatgc cacgcataga agtggaaccg gacacgttca cggtgcgcat cgacggtgag
     3601 gtctggcagg agcagcccgc ggccgaactg ccgatggcac aacggtattt cctgttctga
     3661 tgacgagctt gtcgacgctt cttgccctgg ccgattcccg gttgccgacc ggcggccatg
     3721 tgcactccgg cggtgtcgag gaggcggtga ccagcggact ggtcgtcgac ctcgccacgt
     3781 tgcgggcctt cctgcgtcgc cgcatcgcca ctcaggggct ggtgaccgcg tcgatggccg
     3841 cggcggtaca caccggatcg ctgtgtttcg cggccgccga cctcgaaacc gacgcccgca
     3901 caccggctcc cgctgcccgg caggcctccc gcgcacaggg ccgcgggctg gccaggctgg
     3961 cgcggcgagt gtggcccggc ggcgaatggg atgcgctggg cgccacgccg cacttcgcgg
     4021 tcgccgcggg cgcggtcggt acggcgagcg gcatgacacc gcagcacacc gcgctgtcca
     4081 tcgtgtacac caccatgacc ggctcggcga ccgccgcaca acgattgctg gccctcgacc
     4141 cgggcgacgt cgcggcgctg acgttcgagc tatccgacat gtgtgatcgg accgccgatc
     4201 tggcatgcaa ggaactcgcc gacctgtccg acccgctgct ggacgtgctg gcccagcgcc
     4261 acatcgaacg cgagcgcccg ctgttcgtct cctgagagga ccaaagattc accatgccac
     4321 cgcatttcat cgacggcgag ccgcatcgcc ctcaccctca ccggccgaaa cgagcgcgga
     4381 cagcgggcga gccgttgcgg atcggcgtcg gcggtcccgt cgggtcgggt aagacggcac
     4441 tggtggcggc gctgtgtcgc gagctgcgtg acgaactgtc cctggccgtg ctgaccaacg
     4501 acatctacac caccgaggac
//
LOCUS       DASYLX010001056         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2487312,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001056 DASYLX010000000
VERSION     DASYLX010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2487312"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="mshA"
                     /locus_tag="VGP27_24765"
     CDS             complement(<1..1098)
                     /gene="mshA"
                     /locus_tag="VGP27_24765"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEV7854547.1"
                     /translation="MRLATDLSNAAAGEAPRRVAVLSVHTSPLAQPGTGDAGGMNVYV
                     LQTALGMARRGVEVEIFTRATSSADEPTVRVAPGVLVRNVVAGPFEGLDKYDLPTQLC
                     AFTAGVLRAEATHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAAL
                     ADGDAPEPPLRAVGEQQVVDEADRLIVNTEHEAQQLISLHHADPARIDVVHPGVDLHT
                     FTPGDQRGAREALGLAPDELILAFVGRIQPLKAPDILLRAAARLTDTIANLRVLIAGG
                     PSGTGLAEPDTLVRLAEELGISDRVTFLPPQSREQLVNVYRAADLVAVPSYSESFGLV
                     AVEAQACGTPVVAAAVGGLPVAVRDGVTGTLV"
     gene            complement(1173..2438)
                     /locus_tag="VGP27_24770"
     CDS             complement(1173..2438)
                     /locus_tag="VGP27_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV7854548.1"
                     /translation="MRTTTLRPAPTTAHTKRALLSRHHIVAPSLCVADAAAASVFNAA
                     RSRGPIARDVIAKVTQLSIATVNRQVTALLDAGVLRERADLAVSGAIGRPRVPVEVNH
                     EPFLTLGVHIGARTTSIVATDLFGRTLDVVDTPTPRGAQAAALASLAGSARRYLSRWH
                     RRRPLWAGVTAGGVVDSATGYLDHPRLGWSDAPVGPVIAETLGLPVSVASHVDAMAGA
                     ELLLGTRRASHQATSLYVYARETVGYALSIGGRVHSPASGPGTIAGLPAQSDLLGGTG
                     QLESTVSDEAVLIAARKQRVIPVEGPGSTMTAVLRAARQGNEPARALLAERARVLGEA
                     VALLRDMLNPDDLVVGGQAFTEYPEAMGIVESAYAQRSVLPTRDIRVTSFGNRVQEAG
                     AGVVSLGGIYADPIGAMRRALQRRPEATA"
     gene            2727..>2995
                     /locus_tag="VGP27_24775"
     CDS             2727..>2995
                     /locus_tag="VGP27_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854549.1"
                     /translation="MTTPDADRRVAVVTGASAGIGEATARTLAAQGFHVVCVARREGP
                     ITALADEIGGTAFVADVTDANEVAALTDSLDRVDVLVNNAGGARGL"
BASE COUNT          442 a         1030 c         1110 g          413 t
ORIGIN      
        1 gaccagcgtg ccggtcacgc cgtcgcgcac cgcgacgggc aggccgccga cggcggcggc
       61 gaccaccggg gtgccgcagg cctgcgcctc gacggcgacg aggccgaacg attcggagta
      121 gctcggcacc gcgaccaggt ccgcggcgcg atacacgttg acgagttgct ctcgagactg
      181 cggcggcagg aacgtcaccc gatcggagat acccagttcc tcggccagcc gaacgagtgt
      241 gtcgggctcc gcgaggcccg ttcccgacgg cccgcccgcg atcagcaccc gcaggttcgc
      301 gatggtgtct gtcagccggg cggcggcgcg cagcagtatg tcgggcgcct tcagcggctg
      361 gatgcggccg acgaaggcga gtatgagttc gtcgggggcc agcccgagcg cctcgcgcgc
      421 ccctcgctga tcgccgggtg tgaaggtgtg cagatcgacg ccgggatgaa cgacgtcgat
      481 acgcgccggg tcggcgtggt gaagcgaaat cagttgctgc gcttcgtgtt cggtgttgac
      541 gatcagccga tcggcctcgt cgaccacctg ctgttcgccg accgcgcgca gcggcggttc
      601 gggtgcgtcg ccgtcggcga gcgcagcgtt cttgaccgcg gcgagggtgt gcgcggtgtg
      661 caccagcggc accgcccacc ggtcgcgcgc cagccagccc acctgacccg aaagccagta
      721 gtgcgagtga acgatgtcgt agtagcccgg ctcgtgggtc gcttcggccc gcagcacgcc
      781 cgcggtgaac gcgcacagct gcgtgggcag gtcgtatttg tccagcccct cgaaggggcc
      841 cgcgacgacg ttgcggacca gtacgccggg cgccacccgc accgtcggct catcggccga
      901 cgaggtggcc ctggtgaaga tctccacctc gacgccgcgc cgggccatcc caagcgcggt
      961 ctgcagcaca tagacgttca tgccgcccgc atcgcccgtg ccgggctgcg ccagtgggga
     1021 ggtgtgcacc gacagcacgg ccactcgccg cggagcctcg ccggcggcgg cgttcgacag
     1081 atccgtcgct aggcgcacac agacatgtct acaccgtgtt cagcggcggc cgtcgtcgag
     1141 ttcgggtccg ggcactgggt tccggcgtga cctcaagccg tcgcttccgg cctgcgctgg
     1201 agcgcccgtc gcatcgcgcc gatcggatcg gcgtagatgc cgccgagcga caccacaccg
     1261 gcacccgcct cctggacccg gttgccgaac gaggtgaccc ggatgtcgcg cgtcggcagc
     1321 accgaccgct gcgcgtacgc ggactcgacg atgcccatgg cctcggggta ctcggtgaac
     1381 gcctggccgc cgaccaccag atcgtcgggg ttgagcatgt cgcgcagcag cgcgacggcc
     1441 tccccgagca cccgggcccg ctcggccagc agcgcgcggg ccggttcgtt gccctgccgg
     1501 gccgcgcgca gcaccgcggt catggtggag ccgggcccct cgacggggat cacccgctgc
     1561 ttgcgggcgg cgatgagcac cgcctcgtcg ctgaccgtcg actccagctg gccggtgccg
     1621 ccgagcagat ccgactgggc gggcaggccg gcgatggtgc cagggccgct ggcaggcgaa
     1681 tggaccctgc caccgatcga cagcgcgtaa ccgacggtct cgcgggcgta cacgtagagg
     1741 ctcgtcgcct gatgcgacgc acggcgggta cccagcaaca gctcggcgcc cgccatggcg
     1801 tcgacgtgcg aggccaccga caccggcaag ccgagcgttt cggcgatcac ggggccaacg
     1861 ggagcatccg accagccaag gcgtgggtgg tcgaggtagc cggtggcgct gtcgacgacg
     1921 ccgcccgcgg tgacgccggc ccacagcggg cggcggcggt gccagcggct cagatagcgg
     1981 cgcgcgctgc cggccagcga cgcgagggcg gccgcctggg cgccgcgcgg cgtcggggtg
     2041 tcgaccacgt cgagggtgcg gccgaacagg tcggtggcca cgatgctggt ggtacgcgcg
     2101 ccgatgtgga cgccgagggt gaggaacggt tcgtggttca cctcgaccgg cacccgaggc
     2161 ctgccgatgg caccggacac cgcgaggtcg gcccgttcgc gcagcacgcc cgcgtcgaga
     2221 agcgcggtca cctgacgatt caccgtcgcg atggacagct gggtgacctt ggcgatgacg
     2281 tcacgcgcga tcgggcctcg cgatcgggcg gcgttgaaga ccgacgccgc cgcggcgtcg
     2341 gcgacgcaca gcgacggggc cacgatgtgg tggcgcgaca gcagggcgcg cttggtgtgt
     2401 gcggtggtcg gggctgggcg gagagtggtg gtgcgcacag gatgtcctcg tcagtagtcg
     2461 gctggtgggt cggtgccaca cctggcgact cagcgggact ggcgaatgtc cggactccgg
     2521 tcaggcgcgg cggtatgcgc ggcgacaaca acaacacgcg cgccgtgcgc cgatggcctg
     2581 actactgcgg atcacgtcgt gaacttagca cggccaacta gctctctgcc cggtgacgtc
     2641 ggtcgcccta ggggaaacgg cacgccgact cggggatctg acccgcgtag ccggctagga
     2701 gattcgcgcc aactacagtt ggtcgcatga cgacacccga tgccgaccgc cgggtcgcgg
     2761 tggtcaccgg cgccagtgca ggtataggtg aagcgaccgc gagaaccctt gccgctcagg
     2821 gctttcacgt ggtctgcgtg gcccgccgcg agggccccat cacggcgctt gccgacgaga
     2881 tcggcggcac cgcgttcgtt gcggacgtga ccgacgcaaa tgaagttgcg gcgctgaccg
     2941 acagcctgga ccgggtggac gtgctggtca acaacgccgg cggcgcccgc ggcct
//
LOCUS       DASYLX010001057         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_622375,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001057 DASYLX010000000
VERSION     DASYLX010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622375"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..169
                     /locus_tag="VGP27_24780"
     CDS             14..169
                     /locus_tag="VGP27_24780"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854550.1"
                     /translation="MHRSTLRYRLGRIRDISGRDLQDVEDRLNLHLATRIVRVAGVPL
                     PPEGNGP"
     gene            complement(475..759)
                     /locus_tag="VGP27_24785"
     CDS             complement(475..759)
                     /locus_tag="VGP27_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854551.1"
                     /translation="MNLKKTAVEAAFVTALGVTVFGLGGGVGHATPLSHDPPATTWAE
                     RGHGGWYWGPGGDDGGGGWGAPGYWAPPPPPPPWGYPGYYGGWGNGGWGC"
     gene            complement(1061..1333)
                     /locus_tag="VGP27_24790"
     CDS             complement(1061..1333)
                     /locus_tag="VGP27_24790"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854552.1"
                     /translation="MTTEMLPMGLAPEMGAAVAVALAVATSRFGRKPLLLATLACYSL
                     GNALMAAAPLFGVVALGRTLGGITYAWASEMPSHRIAQCEPCVLLG"
     gene            complement(1451..3331)
                     /locus_tag="VGP27_24795"
     CDS             complement(1451..3331)
                     /locus_tag="VGP27_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854553.1"
                     /translation="MDTVLGVSVTTTTVRMVLIEGEKADGVIIECATFDTAGGVVKAG
                     PHEQISAAIRATQQSALSMGHHLVVSGVAWDDDPTPGTFRDGISARDLQNVMLVAERH
                     ATAALAQNIGRALGFDTTAVMVIGPQTATLSVVDSNDGSLVEARMCTVDGDHVSDILP
                     AMAATLESTNPKPGSVFVVGPRPVVTSVKSSLELLISQPVIVPEEPEFALARGAALAA
                     ASVPGSEASTCGLAYSQDPDELAVLGRAPVKLADAETQVALFDSIRNDDVGPSPQRDP
                     NSKARIPIGSVAAAILVVGAVTLAMSVAGNVRTAIDQDSVPAGAAEVSGPAPELPVAQ
                     AAAVPAPAVPQSVPVATPAALPPPAPPLPAPAFSVPAVPAPAVPAPAPVVQVRRPAPQ
                     RVIAEPAPVVVEKALPAPAPVVVEPEAPEALPEPVGVVAPPPTVSPPPLPPASLPSPV
                     INAPAINPAAPEPPSSSSPWIPPWLRIGTPPSQQQVTQPLPGRQWLPNLSGPQQEVGQ
                     WPQQPSPDLQIVLWPRQQSAPQWTPQVPQWTPQIPQPAAQVPQWTPSLAPRPAAQVPQ
                     WTPSLTPQWTPAPLAPQWTPVPSGQAPQSPATTRSPYTYPNPILPGLLNPAGADNAVP
                     QY"
     gene            3621..4439
                     /locus_tag="VGP27_24800"
     CDS             3621..4439
                     /locus_tag="VGP27_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEV7854554.1"
                     /translation="MLNRATAAFALLAAPVAVIAGCSNQTTNEQTSSTSAASGDQTIK
                     AELKSADGTPVADATIDFTSGYATVTVETVKAGVLSPGSHGMHIHSVGKCEANSVAPT
                     GGASGNFLSAGGHLQVGGRTEHPASGDLTPLNVRSDGSGKVVATSDAFTVDDLKGLEG
                     SALIIHEKPDNFANIPPRYTHDGVPGPDAETLATGDAGGRVACAVLAPATAAATTSVS
                     TSTSTVTETSGVPVPPASSPTTSSTSSSTTATTTTPSTSVTTSTSPPTSPAPAG"
     gene            4779..4973
                     /locus_tag="VGP27_24805"
     CDS             4779..4973
                     /locus_tag="VGP27_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEV7854555.1"
                     /translation="MIRTAFVAAAIAAAAIGIAPVAAASGQYANCTQAHKDGRYDIPQ
                     GDPDYWSGGDRDGDGIACES"
     gene            4979..5458
                     /locus_tag="VGP27_24810"
     CDS             4979..5458
                     /locus_tag="VGP27_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854556.1"
                     /translation="MRLGVGGGAFGVRGGISTRGLGVGVGPFSAGTSWRRGGSGGGGF
                     FAWLVVAMFLILIVAWPYLLGTYIAVQLGASDPSTARAAIGWFFEVLYIAGLIAWFVL
                     SREKRAQQVRDEAQRFAHLAASGAVFQTQVGRTLAYRHRACTVNHRSHATAERCPNG"
     gene            5463..>5680
                     /locus_tag="VGP27_24815"
     CDS             5463..>5680
                     /locus_tag="VGP27_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HEV7854557.1"
                     /translation="MTANVRTCATAIAIALMVATILTSPLTATADPVTVTATVLKIVD
                     GDTIDIRDDVRGRLRVRLLGIDTPETKKP"
BASE COUNT         1018 a         1850 c         1837 g          975 t
ORIGIN      
        1 gcagtcgctc actatccaca gaagcacttt gcgctatcgg cttggacgta ttcgcgacat
       61 ctcggggcgt gacctgcagg acgtcgagga ccgactgaac ttgcatttag cgacccggat
      121 tgtccgcgtt gccggggtgc ccctgccgcc ggaaggcaac gggccctagg cgtctctgcg
      181 actatcggcc ccacacgaac gaccattacg ccccatggtg acgcgggccg acgggtcaaa
      241 gatcatcaag tactgcagca cgccttgctg gtcctccttc cgacgcgatc aggagaaact
      301 gcctgagggc cggcgcgaga catcgcgctg cgttacgagg gcatgctcaa tgcaatggcc
      361 atcccgctga acaccgaacc tggtcgctcg ggccatgcga atgacgcgcg cgttactgga
      421 cgacggttga tcacggtctg cgaagcgcgt ttcgactaca gggagtgacg gccgtcaaca
      481 cccccagccg ccgttgcccc agcccccgta atagcccggg tacccccatg gcgggggagg
      541 cggtggaggt gcccaatacc ctggggcgcc ccagccgcca cctccgtcgt cccctcctgg
      601 accccagtac caaccgccat gtccgcgttc ggcccaggta gtcgccggcg ggtcgtgcga
      661 cagcggagtc gcatgtccca caccgccgcc taggccgaac accgtgacgc cgagcgccgt
      721 gacaaaggcc gcctcgacag ctgttttctt cagattcatg ggtggcacct cgttcatagc
      781 cagaccccca ggcggccaag cgattgacat ccatgcccct cggccgctgg ggccgagaat
      841 actccttcgc gtgtcgaccc gacacggctt gaaagcccgg aatgtcccaa ttccttgcta
      901 tcgcaacgac atctggcccg tcacgcggag gcgagtgcgc caacgtgaag atcgtcgggc
      961 ggtgagcagg gggcatcccg cgtatcggcg gaaggggcgt caggcagttt cgccaggccc
     1021 ggaccgactc agccgagcaa ggcgggtagt ggcatatgca tcaacccaaa agcacgcacg
     1081 gctcacactg tgcgattcga tgactgggca tttcgctcgc ccaggcgtac gtgattccgc
     1141 cgagggtccg tccgagcgca acaacgccga acagcggagc ggcggccatc agcgcattgc
     1201 ccaacgagta gcaagccaag gtcgccagca gaagtggctt gcggccgaac cgggacgtcg
     1261 caacggcaag ggcaaccgcg acggccgcgc ccatctccgg cgccagcccc atgggcagca
     1321 tctccgtggt caccgcgccg aattaggccg cggcccaccg tggatcgcca acgcgacaag
     1381 ctcaccgacc aaagcgggcc gaaccccgat ttcaccagcc cgcagcttgg ccctgatcaa
     1441 acatccgcga tcagtattgg ggcacagcgt tatcggcgcc ggctgggttc aatagtccag
     1501 gcagtatcgg gttcggatac gtgtagggcg aacgcgttgt cgccggactt tgcggagcct
     1561 gccccgaggg gacaggtgtc cactgtggtg ccagtggcgc gggagtccat tgcggggtca
     1621 gcgatggcgt ccactgcggc acctgcgctg ccggccgcgg ggccagcgat ggcgtccact
     1681 gcgggacttg cgctgccggc tgcgggattt ggggtgtcca ctgcgggact tggggcgtcc
     1741 actgcggcgc tgactgctgt cgaggccaaa gaacaatctg cagatcgggc gacggctgtt
     1801 gcggccattg cccgacctct tgctggggcc ctgagagatt cggcagccat tgacgacccg
     1861 gaagcggttg agtgacttgc tgttgggatg gtggtgtccc aatccgcagc cacggtggga
     1921 tccacggcga tgacgaggaa ggcggctccg gtgcggcggg attgatagcc ggtgcattga
     1981 tgaccggcga cggcagcgag gctggtggta atggtggcgg ggacaccgtc gggggcggcg
     2041 ctacgacccc aaccggctca ggaagcgctt cgggtgcttc gggctcgact acaacgggcg
     2101 cgggtgctgg cagtgctttt tcaaccacga ccggggctgg ttcagcgatg acacgctgcg
     2161 gagcaggtcg acgaacctgg acgacgggtg caggcgccgg gactgcaggc gccgggactg
     2221 cgggcaccga gaatgccggt gccggcaatg gcggggcagg tggaggcagc gccgctggcg
     2281 tcgcaaccgg cacgctttgc ggaacggcag gggcaggaac tgcggcggct tgcgcgacgg
     2341 gtagctcggg cgcaggaccg ctcacctcag ccgctccggc tggaacggag tcctggtcaa
     2401 tggcggtgcg gacgttgccg gcaaccgaca tcgccagcgt caccgcaccg accaccagga
     2461 tcgcggccgc gacgctcccg atggggattc gcgccttgga attggggtcg cgctgcggtg
     2521 aggggccgac atcgtcattt cggatggagt cgaacagggc cacctgtgtt tccgcgtcgg
     2581 ccagcttgac cggggctcga cccagtacgg caagttcatc tggatcctgc gaataggcca
     2641 gaccgcacgt ggacgcctcc gagccgggga cactggccgc ggccaatgcc gcgccacggg
     2701 cgagggcgaa ttccggctcc tccgggacga tgaccggctg cgaaatcagc agttcgaggc
     2761 tcgacttcac cgacgtgacc accggtcgtg gacccaccac aaacacactg ccgggcttgg
     2821 ggtttgtgga ctccaaggtg gccgccatcg ccggcaggat gtcgctcaca tggtcaccgt
     2881 ccacggtgca cattcgcgct tccacaagtg agccgtcgtt gctgtcgacg accgacaacg
     2941 tcgcggtctg cggtcctatc accatcactg ctgtggtgtc gaaaccgagt gcacgcccga
     3001 tgttctgggc caacgcggct gtggcgtgcc gctccgccac cagcatcacg ttctgaagat
     3061 cgcgcgcgga gatgccgtcg cggaaggtcc ccggcgtcgg gtcgtcgtcc cacgcaacgc
     3121 cgctcaccac caagtggtgg cccattgaca aggcgctctg ctgggttgct ctgattgcgg
     3181 cgctgatctg ttcgtgcgga ccggctttga cgacgccgcc ggcggtatcg aacgtggcgc
     3241 attcgatgat gacgccatca gctttttcac cctcgatgag caccattcgc acggtggtgg
     3301 tcgtcaccga cacaccaagc acagtgtcca tggcccctcc gaaagttccg gcgtgcagtt
     3361 cccgtacagg aggcgttaat ttgacgctac gccggcgggc ccattcgtat ggcgaatcgg
     3421 cggactcttt ctttaatagg cctttcgtag gtattttcgg cgcgtcagac ttttcgcttg
     3481 gggtcgcgcc gttgtggtag caatccttgg attattgcca ctggcgtctg ggcaatccgc
     3541 ccagtgtcgg ttcgcgtccc tctgagggca cggcaccgac gtgccgatat ggatccgctc
     3601 aatctgggta ttcgaccgtg atgttgaacc gcgcaaccgc agccttcgcc ctgctagcag
     3661 cacccgtagc cgtaatagcc ggctgctcca accaaacgac caatgagcag acgtcctcga
     3721 cgtccgccgc atcaggcgac cagaccatca aggccgagtt gaaatctgcc gatggcacac
     3781 cggtcgctga tgcgacgatc gacttcacga gcggctatgc aacggtgacg gtggaaaccg
     3841 tcaaggccgg ggtgctgtcc ccgggcagcc acggcatgca cattcactcc gtgggcaagt
     3901 gcgaagccaa ctccgtcgct ccgaccggcg gtgcgtcggg caacttcctt tccgccggag
     3961 gtcatttgca ggtcggaggt cgcaccgagc atccggcaag cggcgacctg acaccactga
     4021 atgtccgctc ggacggttcc ggaaaagtcg tcgcaaccag cgacgcgttc accgtggacg
     4081 acctgaaagg tctcgaagga agcgccctga tcattcacga gaaacctgac aacttcgcca
     4141 acatcccgcc gcgctacacc cacgacggcg tgccgggccc agacgcggag acactggcga
     4201 ccggcgacgc cggtgggcgg gtggcgtgtg ccgtcctcgc gcccgccacc gctgccgcca
     4261 ccacgtcggt ctccacgagc acctcgaccg tcacggagac gagcggcgtt ccggtgccgc
     4321 ccgcttcgag tcccaccacg tcgtcaacgt cgtcgtcgac caccgccacc accacgacgc
     4381 cttcgacctc ggtgacgacc tcgacatcgc cgccgaccag tccggcgcca gccggctgag
     4441 acggcccgca cgcctgcctc ggcaggtgtt cggcccggat tgacctcgga tgaacccaat
     4501 tagggataca tcgggccggt tcgtactgtc taatgcatcg gggaccgcac gaaggtcacc
     4561 gaatccgctg aggtctcagc aacctaatag ccgcgtcgag aagctccccc acacgctgca
     4621 gacggcaagc gcactttatt tgcgatcttg atttttctcg tcggcttccg gaaccgagaa
     4681 atcccccttc aacagctaat ttgttattcc gtcaattaac aactaatctt ttgctgcccg
     4741 gggagatcaa gtgaccggca ccacaccaag gggagcaaat gattcgaacg gcttttgtcg
     4801 ccgccgccat agccgcagcg gccatcggta tcgctccggt tgccgccgca agcggacagt
     4861 atgcgaactg cacacaggcg cataaggatg ggcgatacga catcccgcag ggcgacccgg
     4921 actactggtc gggcggggat cgtgacggcg acggtatcgc ctgcgagtcc tgaccacgat
     4981 gcgactgggg gttggcggcg gcgctttcgg cgtccgtgga ggcatctcga ctcgagggct
     5041 gggggtcggt gtcggcccgt tttctgcggg cacgtcgtgg cggcgggggg gttcgggagg
     5101 cggcgggttc ttcgcctggc tggtggtcgc catgttcctg atcttgattg tcgcgtggcc
     5161 atacctattg ggtacctaca tcgccgtcca actcggcgcg agcgatccgt cgacagcccg
     5221 cgcggcgatc ggctggttct tcgaagtgct ctacatcgcc ggcctgatcg cctggttcgt
     5281 attgagcaga gagaagcgag cacagcaggt tcgggatgaa gcgcagcggt tcgcccacct
     5341 cgccgcgtca ggggccgtct tccagacgca ggtgggtcgc acgctggcgt atcggcaccg
     5401 agcatgcacc gtgaaccatc ggtcgcatgc cacagcggaa aggtgcccca acggctagca
     5461 ccatgacggc gaatgtccgc acctgcgcga ccgcgatcgc tatcgcacta atggtggcga
     5521 caatcctgac ctcgccgctg accgccactg ccgatcccgt caccgtcaca gcgacggtgc
     5581 tgaaaatcgt cgacggagac accatcgaca ttcgcgacga tgtgcggggc cggcttcgcg
     5641 tgcggctctt ggggattgac acaccggaga ctaagaaacc
//
LOCUS       DASYLX010001058         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3036081,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001058 DASYLX010000000
VERSION     DASYLX010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036081"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGP27_24820"
     CDS             <1..322
                     /locus_tag="VGP27_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854558.1"
                     /translation="QRRLDRRLNMPLFDTDISAVATGLDDPPPLLVVHDPDDPDSPHA
                     MSENLVGVWPGASLVTTRGLGRLAHYRILRHRPAIQAALDFIGRAPSRADAQETAAAH
                     VMPQ"
     gene            complement(747..>1823)
                     /locus_tag="VGP27_24825"
     CDS             complement(747..>1823)
                     /locus_tag="VGP27_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV7854559.1"
                     /translation="SVLQMKSGQTITIDTVSHEGILEDQGRNPVEYFGSHGVAESNVL
                     QDAIDIASGYSRTPRNFDVDGPHVVTGPVFVDGAQPGDVLKIETLEATPRVPYGVVSS
                     RHGKGALALSAKGEAPAGIGRDEVMPPVATDGRQTKDPMKYGNVSTFTAVENGHGVMP
                     FGDAAVRFPLRPFMGMMGVAFASAPGLTSPNANSVPPTLGGGNIDISLLGAGSTFYLP
                     VFADGALFYVGDPHMAMGDGEVALTAMEGALRGTFRLTVCKKGSGDAPSVAYLYPFAE
                     NQDSWIPIGLSDPDGAANGQSNDLNVAMRRAIVNALNFLEDDRGMDRATAYAYLSAAA
                     NFSVSQVVDKTVGVHAQIAKDHFA"
BASE COUNT          332 a          607 c          564 g          320 t
ORIGIN      
        1 gcagcgccgc ctcgaccgac ggctgaacat gccgctgttc gacaccgaca tttccgcggt
       61 ggcgaccggt ctcgacgatc cgccgccgct cctggtggtg cacgaccccg acgatcctga
      121 cagcccccat gcgatgagcg agaaccttgt cggcgtgtgg cccggagcaa gtctggtgac
      181 cactcgcggg ctcggccgtc tggctcacta ccgaatcttg cggcaccgcc ccgcgattca
      241 ggccgccctg gatttcatcg gccgtgcgcc cagccgcgcc gacgcgcagg aaacggcggc
      301 cgcacacgtg atgccgcagt agcgaaaaaa ttcagcgccc gtcgccttct gcgcaggacc
      361 ggacccgcaa cgtagccgca gacgaaacca agtaggaaga cccggcgcgc ttttcgcctg
      421 ttccgccgta agacggctga cccgatgaat ccacctgtcg ctgctacgac ggccacgacg
      481 agtagcacag cgagtagatg tgccggtccg agcggcccca tatcccaacc tccgggccgt
      541 cgtccgggtc ggccgcttgc tcgatccaac cagcgaaatc gccggaccag aatcatcgga
      601 cactgtttgt gaccaaagcg attgccaagc gttaggccga acgacagggg cgatccgcac
      661 cgaccggcct ctggacgacg tcgacgtctg cgcggtccag gccgaactgc tccgccagga
      721 cgtccggatc gcctagttag tcccgtttag gcgaagtggt ccttcgcgat ttgcgcgtgc
      781 acgccgacgg tcttgtccac cacttggctg acggagaagt ttgcggcagc agacaggtat
      841 gcatacgccg tggcgcgatc catcccacgg tcgtcttcca ggaagttcaa cgcgttgacg
      901 atagcgcgtc gcatcgcgac gttcagatcg ttactctgac cgttcgccgc accgtcagga
      961 tcagacagtc cgatcggaat ccatgaatcc tggttctcgg caaagggata caggtaggcg
     1021 accgatggtg catcgccact gcccttcttg catacggtca accggaacgt accccgcaac
     1081 gcgccttcca tggccgtgag ggcgacttcg ccatctccca tcgccatgtg aggatcaccg
     1141 acgtagaaca gcgcgccgtc cgcgaacacg gggaggtaga acgtcgagcc tgcgcccagc
     1201 aggctgatgt cgatgttccc tccgcccaag gttgggggga cggagttggc gttcggcgac
     1261 gtcaagccgg gagcgctcgc gaaggcgacg cccatcatcc ccatgaacgg ccgcaacgga
     1321 aagcgaactg cggcatcgcc gaacggcatc acgccgtggc cgttctcgac ggcggtgaat
     1381 gtggaaacgt tgccgtactt catcgggtcc ttggtctgtc tgccatcggt ggccacgggc
     1441 ggcatgactt catctcggcc gatacctgca ggcgcctcgc ccttggcgct caaggccagc
     1501 gcccccttgc cgtggcggct ggagaccaca ccgtatggaa ccctgggtgt ggcctcgagg
     1561 gtctcgatct tcaagacgtc gccgggttgg gcgccgtcga cgaagacggg accggtgacg
     1621 acatgcggtc catccacgtc gaaattgcgg ggcgtgcggc tgtaccccga ggcgatgtct
     1681 atcgcgtcct gcagcacgtt tgactcggct acgccgtggg agccgaagta ctcgacggga
     1741 ttgcgaccct gatcttccag gattccctca tgcgagaccg tgtcgatggt gatcgtctgc
     1801 cccgacttca tctgcaggac cga
//
LOCUS       DASYLX010001059         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3036207,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001059 DASYLX010000000
VERSION     DASYLX010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036207"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VGP27_24830"
     CDS             <1..162
                     /locus_tag="VGP27_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV7854560.1"
                     /translation="QVLHGRVRVTDTKAHWDGSPGDHIVIPTSRHGLHAIEDSAVLLT
                     VVKVVGPHV"
     gene            complement(159..1808)
                     /locus_tag="VGP27_24835"
     CDS             complement(159..1808)
                     /locus_tag="VGP27_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEV7854561.1"
                     /translation="MTDGPLIVQSDKTVLLEVDHEQAGAARAAIAPFAELERAPEHIH
                     TYRITPLALWNARAAGHDAEQVVDVLVSFSRYAVPQPLLVDIVDTMARYGRLQLVKSP
                     VHGLTLVSLDRAVLEEVLRHKKIAPMLGARIDDDTVAVHNSERGRVKQMLLKIGWPAE
                     DLAGYVDGEKHPISLDQQNWHLRDYQEMAADSFWQGGSGVVVLPCGAGKTLVGAAAMA
                     KAGATTLILVTNTVAGRQWKRELINRTSLTEEEIGEYSGEKKEIRPVTIATYQVITRR
                     TKGEYRHLELFDSRDWGLIIYDEVHLLPAPVFRMTADLQSRRRLGLTATLIREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGWIAPAECIEVRVTMTDNERMLYAVAEPEERYKLCST
                     AHSKIAVVKSILERHPGDQTLVIGAYLDQLDELGRELNAPVIQGSTKTAEREALFDEF
                     RRGEIPTLVVSKVANFSIDLPEANVAIQVSGTFGSRQEEAQRLGRLLRPKADGGGAIF
                     YSVVSRDSLDAEYAAHRQRFLAEQGYGYVIKDADDLLGPAI"
     gene            complement(1943..>2897)
                     /locus_tag="VGP27_24840"
     CDS             complement(1943..>2897)
                     /locus_tag="VGP27_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7854562.1"
                     /translation="AMRGKGAPAHGALVPVDIPDDASGFKHRTELVYLPPAWFAANAP
                     RLPVVMMIAGEFNTPSDWPRTGDAIAPIDKFAAAHNGNAPVFVFVDVGGSFNNDTECV
                     NGPRGNVADHLSKDVPPYLSSTFGVQNADWGVVGWSMGGTCAVDLTVMHPDLFSTFVD
                     IAGDMGPNAGTKEQTIARLYGGNTAEWATYDPTTVITEHGPYRGVSGWFAISSDAPQQ
                     RNGGSGHAGGTGLGGQDAAMNPGDQTDAANSLCNLGRANGIACAIVAQPGRHDWPLAA
                     DVFTSALPWLAGQIATPGVPQIPLPGQGTGPAGTPLPMATH"
BASE COUNT          433 a          966 c          982 g          516 t
ORIGIN      
        1 caggtgctgc atggcagagt ccgcgtcacc gacacgaagg cgcattggga tggctcacca
       61 ggggaccaca tcgtgatacc gacgtcgcgg cacggtctgc acgcgatcga ggactcggcc
      121 gtgttactga ctgtggtgaa agtcgttggg ccgcacgtct aaatcgcagg gcccagcagg
      181 tcgtcggcgt ccttgatgac gtagccgtag ccctgctcgg cgaggaaacg ctgacggtgc
      241 gcggcgtatt cggcgtccag gctgtcgcgt gacaccaccg agtagaagat cgcgccgccg
      301 ccgtcggcct tgggccgcag cagcctgccc aaccgctgcg cctcttcttg gcgcgacccg
      361 aatgttcccg aaacctgtat cgccacattg gcttccggta gatcgatgga gaagttcgcg
      421 accttcgaca ccaccaacgt gggaatctcg ccgcgacgga actcatcgaa cagcgcctcg
      481 cgttcggcgg tcttcgtcga cccctgaatc accggagcgt tcagctcccg gcccagctcg
      541 tcgagctgat cgagataggc accgatcacc agcgtctgat cgccggggtg gcgttccagg
      601 attgatttca ccaccgcgat cttggaatgc gccgtcgagc agagcttgta ccgctcctcc
      661 ggctcggcaa ccgcgtagag catccgctcg ttgtcggtca tcgtcacccg cacctcgatg
      721 cactccgccg gcgcgatcca gccctgtgcc tcgatgtcct tccacggcgc gtcgtagcgc
      781 ttgggcccga tcaggctgaa gacgtcgccc tcgcgtccgt cctcgcggat cagggtggcc
      841 gtcaagccga gcctgcgccg ggactgcagg tctgccgtca tccggaagac cggcgcaggc
      901 agcaggtgca cctcgtcgta gatgatcagc ccccagtcgc ggctgtcgaa cagctcgagg
      961 tgccgatact cgcccttcgt gcgccgggtg atcacctgat acgtggcgat ggtgacgggg
     1021 cggatctcct tcttctcgcc ggagtactcg ccgatctctt cctcggtcag cgacgtgcgg
     1081 ttgatcagct cgcgcttcca ttgtctgccc gcgacggtgt tggtgaccag gatcagcgtc
     1141 gtcgcgcctg ccttggccat cgcggcggcg ccgaccagcg tcttgcccgc accgcacggc
     1201 agcacgacga cacctgagcc gccctgccag aacgagtcgg cggccatctc ctggtagtcg
     1261 cgcagatgcc agttctgctg gtcgaggctg atggggtgct tctcgccgtc gacgtagccg
     1321 gcgagatcct cggcgggcca accgatcttg agcagcatct gcttgacgcg gccgcgctcg
     1381 ctgttgtgca ccgcgacggt gtcgtcgtcg atgcgggcgc ccagcatcgg tgcgatcttc
     1441 ttgtgccgaa gcacctcctc gaggaccgcc cggtcgaggc tgacgagggt gagtccgtgc
     1501 acggggctct tcaccaactg aaggcggccg taccgggcca tggtatcgac gatgtcgacc
     1561 agaagcggct gcggcaccgc gtagcgcgag aacgacacca gcacgtcgac gacttgttcg
     1621 gcgtcgtggc cggcggcacg ggcgttccac agcgccagcg gcgtgatccg gtaggtgtgg
     1681 atgtgctcgg gcgcgcgttc cagctccgcg aacggggcga tcgcggcgcg tgctgcgcct
     1741 gcttgctcgt ggtcgacttc gagcagcacc gtcttgtcgg actgcacgat cagggggccg
     1801 tcggtcattt tgtccattat ccctactcga ccgacgccgg ccgcgacgag tgtgcgtgaa
     1861 ctgcaatttt cggcggcgtg tcggtctcag acacgctccc cctcgcaacg cggggggtgc
     1921 ccccagctcg cggtggggag tgtcagtggg tggccatcgg cagcggcgtg cccgccgggc
     1981 ctgtgccttg gcctggcagc gggatctgcg gcaccccggg tgtagcgatc tggcccgcca
     2041 gccatggcag cgccgaggtg aacacgtcgg cggccagcgg ccagtcgtgg cggcccggct
     2101 gcgcgacgat ggcacacgcg atgccgttgg cgcggccaag gttgcacagc gaattggcgg
     2161 catcggtctg atcgccgggg ttcatcgcgg cgtcctgccc gccgaggccg gtgccgccgg
     2221 cgtgtccgga gccaccattg cgctgctgcg gcgcatcgga ggagatcgcg aaccaacccg
     2281 acacgccgcg gtagggcccg tgctcggtga tcaccgtggt ggggtcgtag gtggcccact
     2341 ccgcggtgtt gccgccgtac agccgggcga tcgtctgctc cttggtgccc gcattgggcc
     2401 ccatgtctcc cgcgatgtcg acgaaggtgc tgaacaggtc gggatgcatc acggtcaaat
     2461 ccaccgcgca ggtgccgccc atggaccagc cgacgacccc ccagtcggcg ttctggacgc
     2521 cgaaagtgct tgacaaatag ggcggtacgt ccttgctgag gtgatcggcc acattgccgc
     2581 ggggtccgtt gacgcactcg gtgtcgttgt tgaacgagcc accgacgtcg acgaagacga
     2641 acaccggcgc attgccgttg tgggcggccg cgaatttgtc gatcggcgcg atcgcgtcgc
     2701 cggtgcgcgg ccagtccgac ggggtgttga actcgccggc gatcatcatc accacgggca
     2761 gcctcggggc gttcgcggcg aaccacgcag gcggcaggta caccaactcg gtgcggtgtt
     2821 tgaagcctga cgcgtcgtct ggaatgtcga ccggcacgag cgccccgtgc gcgggcgcgc
     2881 ccttgccgcg catggcc
//
LOCUS       DASYLX010001060         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2816752,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001060 DASYLX010000000
VERSION     DASYLX010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816752"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGP27_24845"
     CDS             <1..138
                     /locus_tag="VGP27_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEV7854563.1"
                     /translation="LAIGVTSYNGMLYFGINADRDAMSDVDVMPSLLRESLDELVEAA
                     Q"
     gene            250..1935
                     /locus_tag="VGP27_24850"
     CDS             250..1935
                     /locus_tag="VGP27_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEV7854564.1"
                     /translation="MPASPNGQAEPHPIFAVPIDDEVHTRNRGLDKVVFGVTAVIAVA
                     FLVWGFVSTSSLARASNSALGWIMDNTGWLFALTASGFVVFVLWLALGRFGGIPLGRD
                     DEEPEFRTISWVAMMFSAGMGIGLMFFGVSEPLTHFATPPPGTGGPGNPNAVQTAMAT
                     TLFHWTLHPWAIYAVVGLAIAYGVYRRGRMQLISSAFEPLLGKRANGMGGKVIDMLAI
                     FATLFGSAASLGLGALQIRSGLHIVAGVSETGNTVLIVIITVLTVAFVLSAVSGVARG
                     IQWLSNINMVLAVLLAGFVFVVGPTVFILNLLPTSLGSYFADLAMMSARTGAEGPDVN
                     TWLQSWTIFYWAWWISWTPFVGMFIARISRGRTIRQFVTGVLLVPSLVSLVWFCVFGG
                     AAINVQQGGVDLAGEGSIERQLFSLLDQYPIAIIASVLVMVLVAIFFVSGADAASIVM
                     GSLSERGTIKPSRGTVIFWGVAMGAVAAVMLLVGGQDALTGLQTITIIAALPFVLVMV
                     GLAVALVKDLRTDPLMVRRRYAAKAVDTAVVHGVTEHGDDFMISVEKDPNRDR"
     gene            1942..2451
                     /locus_tag="VGP27_24855"
     CDS             1942..2451
                     /locus_tag="VGP27_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854565.1"
                     /translation="MVRVYVPATLAMLQQLVANGSVSAVNGTAFAVTPALRESYAEGD
                     EDELADVALREAALASVRLLAGAAGDAAAEQRPRRAVLVIEAEGATLRPDLDDAVVRL
                     SGPVAIGDVVAAYVDNEAAESAVLAAIDAVDAADLGDEDAELTVGDAQDHDLAWYAPQ
                     ELQFLLDLL"
     gene            2503..>2847
                     /locus_tag="VGP27_24860"
     CDS             2503..>2847
                     /locus_tag="VGP27_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEV7854566.1"
                     /translation="MIKVSANVADTVRPRIAGAKEHPGWHALRTIAGRITTPLLPDDY
                     LKLANPLWSARELRGRVLEVRRETVDSATLVIKPGWGFGFNYQPGQYVGIGLLVDGRW
                     RWRSYSLTSSPIK"
BASE COUNT          438 a          911 c          951 g          547 t
ORIGIN      
        1 ttggcgatcg gcgtcacgtc ctacaacggg atgctctact tcggcatcaa cgccgaccgc
       61 gacgcaatga gcgacgtcga cgtaatgccg tccttgctgc gggaatcgct cgacgagttg
      121 gtggaagccg ctcagtaaca ctttgtttgc gtcggtcggc ttgacggccg acgtcaacag
      181 gtctcgaatg aattcaccat gacattcagc tctgaatctc ctgagacttc cggagagtcg
      241 gcggccggga tgccggcctc cccgaacggc caggccgaac cgcatccgat cttcgctgtg
      301 ccgatcgacg acgaggtgca cacccgcaac cgcggactcg acaaggtggt cttcggtgtc
      361 accgccgtca tcgccgtcgc gtttttggtc tgggggttcg tcagtacgag ttcgctggcc
      421 agagcgtcca attcggctct cggctggatc atggacaaca cgggctggct gttcgcgctg
      481 accgcctccg gtttcgtggt cttcgtgctc tggctggcgc tcggcaggtt cggcggcatt
      541 ccgctcggcc gcgacgacga agaacccgag tttcgcacca tctcgtgggt ggcgatgatg
      601 ttcagcgcgg gcatgggcat cggcctgatg ttcttcggcg tctccgagcc gctcacccac
      661 ttcgccacgc cgccgccagg cacaggcggg ccgggcaacc cgaatgcggt gcagaccgcg
      721 atggcgacga cgttgttcca ctggacgctg cacccttggg cgatctacgc ggtggtgggg
      781 ctggccatcg cctacggcgt gtaccgcagg ggccggatgc agctcatcag ctccgcgttc
      841 gagccgctgc tgggtaagcg tgccaacggc atgggcggca aggtgatcga catgttggcg
      901 atcttcgcca ccctcttcgg ttcggcggcc tcgctcggac tgggtgcgct gcagatccgc
      961 agcggcctgc atatcgtcgc gggagtcagc gaaaccggca acacggtcct gatcgtcatc
     1021 atcacggtcc tcaccgtggc attcgtgttg tcggccgtgt ccggtgtggc acggggcatt
     1081 cagtggctgt cgaacatcaa catggtgctc gccgtgttgc ttgccgggtt cgtattcgtc
     1141 gtcggcccaa ccgttttcat cctcaacctg ctgccgacct cactcggcag ttacttcgcc
     1201 gacctcgcga tgatgtcggc gcgcaccggc gctgaaggac ccgacgtcaa cacctggctg
     1261 cagtcctgga ccatcttcta ctgggcctgg tggatctcct ggacaccgtt cgtcggcatg
     1321 ttcatcgcgc ggatctcgcg cgggcggacg atcaggcaat tcgtcactgg ggtgctgctg
     1381 gtgcccagct tggtgtcgct ggtgtggttc tgcgtgttcg gtggcgcggc gatcaacgtc
     1441 cagcagggcg gcgtcgacct cgcgggcgag ggcagcatcg agcgccagct gttcagcctg
     1501 ctcgaccagt acccgatcgc catcatcgcc agtgtgctgg tcatggtgct ggtggccatc
     1561 ttcttcgtct ccggagccga cgccgcctcg atcgtgatgg gttcgctgtc cgaacgcggc
     1621 accatcaaac cgagtcgcgg cacggtcatc ttctggggcg tcgccatggg tgccgtcgcc
     1681 gcagtcatgc ttctggtcgg cggacaagac gcgctcaccg gccttcagac catcacgatc
     1741 atcgccgccc tgccgttcgt tctggtgatg gtgggtctgg ccgtcgcgct ggtcaaggat
     1801 ctgcgcactg acccgctcat ggtgcggcgc aggtacgccg cgaaggcggt cgacaccgcg
     1861 gtggtgcacg gagtgaccga acacggcgac gacttcatga tctcggtcga gaaggatccc
     1921 aaccgggacc gctaatctcg gctggtgcgt gtctatgtgc ctgcgaccct ggcaatgctg
     1981 cagcaactcg ttgccaacgg gtcggtcagc gccgtgaacg gcaccgcgtt cgcggtgaca
     2041 cctgcgctac gggagtcgta cgccgagggc gacgaggacg aactcgccga cgtcgcgctg
     2101 cgcgaggcgg cgctggcctc ggtgcggctg ctggccggcg ctgctgggga cgctgccgcc
     2161 gagcagcggc cgcggcgggc ggtgctcgtc atcgaggccg agggcgcgac tctgcgaccc
     2221 gacctcgacg acgccgtggt gcggttgtcc gggccggtcg ccatcggaga tgtggtcgcc
     2281 gcctacgtcg acaacgaagc cgccgagagc gccgtgctgg ctgcgatcga cgccgtggat
     2341 gccgccgatc tgggcgacga ggacgccgaa ctgaccgtcg gcgacgctca agatcatgat
     2401 cttgcctggt atgccccgca ggagctgcag ttccttctcg acctgctcta ggggtggata
     2461 cgacagcgta agttacggta ccgtaggttc gtggcgaaga acatgatcaa ggtctcggcc
     2521 aacgtcgccg ataccgtgcg accgaggatc gcgggcgcca aggaacatcc aggctggcac
     2581 gcgttgcgca ccatcgccgg acgcatcacg acgccactgc tgcccgacga ctacctcaaa
     2641 cttgccaacc cgctgtggtc cgcccgtgag ctgcgtggac gagtgctgga agtgcgcagg
     2701 gagacggtcg actccgcgac gctggtgatc aagccgggct ggggcttcgg cttcaactac
     2761 cagcccggcc agtacgtcgg aatcgggttg ttggtggacg gccgctggcg gtggaggtcg
     2821 tactccctga catcgagccc gatcaag
//
LOCUS       DASYLX010001061         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1280109,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001061 DASYLX010000000
VERSION     DASYLX010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280109"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VGP27_24865"
     CDS             <1..594
                     /locus_tag="VGP27_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854567.1"
                     /translation="LWWVALASVLCLLIVEGAIVNAVLARRDGVTVGTDDDGPGLRLA
                     VIGLAAVALVAAVAIGYTQWTVPDRNLRDDSAEVVGIASSVAEASATFTPASPTAAID
                     RATELMTPDRADKYKNEFAAVAKQLTSKNVTASASTISAGVEAIGPQDASVAVMMRGT
                     QNVPGQPPDMAVLALRITLAKDGDRWLVDDVTPINSR"
     gene            complement(581..988)
                     /locus_tag="VGP27_24870"
     CDS             complement(581..988)
                     /locus_tag="VGP27_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV7854568.1"
                     /translation="MAADIDRILRETRTVAIVGASANRVRAVYGVWKYLESASDYECY
                     LVNPTVSDIDGTPTYPSLAALPVVPDLVDVFRRHEHLPAVLADAIGVGAKTLWLQSGL
                     WDEQVARDGEAAGLQVVMDRCLKVEYARLLSVS"
     gene            complement(1026..1511)
                     /locus_tag="VGP27_24875"
     CDS             complement(1026..1511)
                     /locus_tag="VGP27_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7854569.1"
                     /translation="MPTGAPKRVRPPIDDDKPVEFWPTAAIREALETDDLTVWQRIVT
                     AIKRDPYGRTARQVEEVLESTRPYGVSKALSEVLTRTRENLEANERAEVARQIRVLLS
                     RSGLKPTEFASRIGVPGEELAGYLDGKTSPPASLMVRMSRLSDRFAKMKAHRPGSPAP
                     H"
     gene            complement(1562..>1756)
                     /locus_tag="VGP27_24880"
     CDS             complement(1562..>1756)
                     /locus_tag="VGP27_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854570.1"
                     /translation="MDRYYCGEPSRKQWVATQLVRRLADPHPSDEFDIPMSGAEAEAD
                     WAKVRERCLSVAVAMLEEAR"
BASE COUNT          280 a          588 c          600 g          288 t
ORIGIN      
        1 ctgtggtggg tggcgctggc gtcggtgctg tgcctgttga tcgtcgaggg cgcgatcgtc
       61 aacgccgtgc tggcgcgccg cgacggggtg acggtcggca ccgacgacga cgggccggga
      121 ctgcggctcg cggtcatcgg cctggccgcc gtggcgttgg tcgccgcggt ggcgatcggc
      181 tacacgcagt ggacggtgcc ggaccgaaac ctgcgcgacg acagcgcgga agtggtgggc
      241 atcgcgagca gtgtggccga ggccagcgcg acgttcacgc cggcgagtcc gacggcggcg
      301 atcgaccggg ccaccgagct catgacgccg gaccgcgccg acaagtacaa gaacgaattc
      361 gcagctgtcg caaagcaatt gacgagcaaa aacgtgacag cctctgccag cacgatttca
      421 gcgggtgtgg aggccatcgg gccgcaggac gccagcgtcg cggtgatgat gcgcgggacg
      481 cagaacgtgc cgggtcagcc gccggacatg gccgtgctgg cgttgcggat caccttggcc
      541 aaggacggcg accgctggct cgtcgacgac gtgacgccga tcaactcacg ctgagcaggc
      601 gggcgtactc gaccttcagg cagcggtcca tcaccacctg caggcccgcg gcttcgccgt
      661 cgcgcgccac ctgttcatcc cacaggcccg actgcaacca cagggttttc gcgccgactc
      721 cgatggcgtc ggcgagcacc gcgggcagat gctcgtggcg ccggaacacg tcgacgagat
      781 ccggtacgac gggcagtgcc gccaaactcg gatacgtcgg cgtgccgtcg atgtcgctga
      841 ccgtcgggtt gacgaggtag cactcgtaat cgctggccga ctcgaggtat ttccacacgc
      901 cgtagacggc gcggacccga ttggcggacg cgccgacgat cgcgacggtt cgggtttcgc
      961 gcaggatgcg atcgatatcg gctgccatga ttaccggtat acccgcgaga tgagaccggc
     1021 ggttcttagt gcggggcggg gctgcccggg cggtgcgcct tcatcttcgc gaagcgatcc
     1081 gacagccggc tcatccggac catcagggat gcgggcgggc tggtcttacc atcgagatat
     1141 cccgcgagtt cctcaccagg cacgccgatg cgcgatgcga actcggtcgg tttgagaccc
     1201 gaccggctga gcaacacccg gatctgccgg gccacctcag cgcgttcgtt ggcttccagg
     1261 ttttccctgg tgcgggtcag cacttcggac agtgccttcg agaccccata cggtcgtgtc
     1321 gactccaaca cctcctcgac ttggcgcgcg gtcctgccgt acggatcgcg cttgatggcg
     1381 gtgacgatgc gctgccaaac ggtcaggtcg tcggtttcca gcgcctcgcg gatcgcggcc
     1441 gtcggccaga actccacggg cttgtcgtca tcgatcggcg ggcgcacccg tttcggggcc
     1501 ccggtcggta tgcgtgcggt ggcggcgtcg cggcgcgcgg cgccgatctc gggagccacc
     1561 gtcacctcgc ctcctccagc attgcgaccg ccaccgacag gcaccgctcc ctgaccttgg
     1621 cccaatcagc ctccgcctcg gcgcccgaca tcggaatgtc gaactcatcc gacggatgcg
     1681 gatcggccaa ccggcgcacc agctgcgtgg ccacccattg cttcctggat ggctcaccac
     1741 agtagtaccg atccat
//
LOCUS       DASYLX010001062         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3694637,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001062 DASYLX010000000
VERSION     DASYLX010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694637"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..>1923)
                     /locus_tag="VGP27_24885"
     CDS             complement(165..>1923)
                     /locus_tag="VGP27_24885"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEV7854571.1"
                     /translation="LPWPDRGRAVAGVSAFGFGGTNAHVVLAEPPQVRETASPDDTVG
                     DRVEILPLSARSPEALAELASRYEVALADGAPLADLCYTAAARRAHHDHRLAAVGRTR
                     DQMRAALIAFREGTFEPALSSGRRRPGRRPGAVFVVTGDGSHWCGMGQQLHAEEPAFR
                     DALAICDRALRPHLEHSVLTEILADPAESHLDDDVDIVAAAVFAVQVSMAALWRSWGI
                     QPAAVIGHGMGEVAAAHIAGVISLDDAARLLCTRTRFIRRRSDRAGRTELKAVLDRLQ
                     PAEATTPLYSTVTGAALNGQLLDDAHWAANVAAPVQISAAIRRLAELGHDTFVEISPN
                     PSMHDEAVSEYTLVSSIHRGESEREAMLAALGGLYSSGQSIAWELLHPWRRRPAAAPM
                     YPWQRERFWLDDLDTDATPSQPRIDVGVPAVDDAPSEEALKVRLDRADQHERLRLLRT
                     YLQTQTAGRLGMRPSLLDIQSPLTNFGVDSLMAAELRTQIERDLGIVVPAVELLDGPS
                     VAGLADWLGSTVSAEGPRKPKKTVSADLLVSTPTGAHQASALAGERWIDLLTQVPDVS
                     DDDVDALLREVLQGGEGGQ"
BASE COUNT          322 a          636 c          653 g          312 t
ORIGIN      
        1 gccgggagcg aggttgtaaa ggaaccacag cgaccgctgt ccgaacgaca gcggatgcgt
       61 cgaggccgac gactccgcgc gttcacggag gagccgcgac aacagcgcac gcttctcatc
      121 ggccgagagg ccggatatgt ctagggcccg atcgatcgtt tcagtcattg tccgccctct
      181 ccaccctgca agacttcgcg aagcagggcg tcgacgtcgt cgtcggacac gtcgggcacc
      241 tgcgtcaata ggtcgatcca ccgttcccct gcgagcgcgc tcgcttggtg agctccagtg
      301 ggagtcgata cgaggaggtc ggcggaaacg gtcttcttcg gtttccgcgg accttctgct
      361 gacactgtcg aaccgagcca gtcggccagg ccggcaacgc ttgggccgtc gagcaattcg
      421 acggccggta ccacgatgcc caaatcacgc tcgatctgcg tccgcagttc ggcggccatc
      481 aatgagtcga cgccgaagtt ggtcagcggc gactggatgt ccagtagcga cggcctcata
      541 ccaagcctgc ccgcggtctg ggtctgtagg taggtccgca ggagccgtag ccgctcgtgt
      601 tgatccgcgc ggtccagtct gaccttgagc gcttcctcgc tgggtgcatc atcgaccgcg
      661 ggcacaccaa cgtcgatccg cggttgactc ggggtcgcat cggtgtcgag gtcgtccagc
      721 cagaaccgtt cacgctgcca cggatacatc ggcgcggccg caggccgacg tctccacggg
      781 tgcagtaact cccatgcgat cgactgaccg ctgctgtaca ggccgccgag cgcggccagc
      841 atggcttcgc gctcggattc accgcggtga atggaagaca ccaatgtgta ctccgaaacc
      901 gcctcgtcgt gcatcgaggg attcgggctg atctccacga aggtgtcgtg tcccagctcg
      961 gccagccggc ggatcgcagc cgagatctgg acgggggcag ctacattcgc cgcccagtgc
     1021 gcatcgtcga gcagttgacc gttcagtgcg gcgccggtca ccgtcgagta caagggtgtc
     1081 gtcgcttcgg ctggttgcag gcgatcgagc acggctttga gctctgtgcg ccccgcgcgg
     1141 tcacttcttc gtcggatgaa gcgtgtccgt gtgcagagca gccgtgcggc atcgtccagg
     1201 ctgatgacac ccgcgatgtg cgcggcggcc acctccccca tgccgtgtcc tatcacggcg
     1261 gcgggctgga tgccccacga ccgccagagc gcggccatgg acacctggac agcgaatacg
     1321 gcggcggcga cgatgtcgac gtcgtcgtcg agatgcgatt cggccgggtc cgccaggatt
     1381 tcggtgagta ccgagtgctc caggtggggc cgcagggcgc ggtcgcagat ggcaagggcg
     1441 tcacggaaag cgggctcttc cgcgtggagt tgctgtccca tgccgcacca gtgcgaaccg
     1501 tcgccggtca cgacgaacac tgcccccggc cgccggccgg gacggcgccg accggacgac
     1561 agggccggct caaaggtacc ttcacggaaa gcgattaggg ccgcgcgcat ttggtcgcgc
     1621 gtccggccga cagccgccaa ccgatggtcg tggtgtgcgc gccgggcagc cgccgtgtag
     1681 cacaggtcgg caagcggagc gccgtcggcg agagccacct cataacgact cgcgagctcg
     1741 gcgagggcct ccggtgagcg cgcggacagc ggcaggattt cgacgcgatc gccaacggtg
     1801 tcgtcggggg aagccgtctc ccgaacctgc ggcggctccg ccagcacgac gtgcgcattg
     1861 gtgccaccga agccgaacgc gctgactccc gcaacggccc ggccgcgatc cggccaaggc
     1921 agc
//
LOCUS       DASYLX010001063         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1938814,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001063 DASYLX010000000
VERSION     DASYLX010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938814"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VGP27_24890"
     CDS             <1..526
                     /locus_tag="VGP27_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV7854572.1"
                     /translation="GEAANAELGLLLGLTGRTLAGELGDRLPALRRDRLVVLGPRDDA
                     WRQRFNVGTLADNGVWLAPLAEVADDPAGAGRRAIEQLAERVDRWWLHIDLDVLDPTV
                     FPAQGLPDFPDEPGGLTWDQLTDMVMALFTTPASCIGGSIAIYDPDQDPNRTGAAQIV
                     AFIRNVVARPTISR"
     gene            539..2716
                     /locus_tag="VGP27_24895"
     CDS             539..2716
                     /locus_tag="VGP27_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7854573.1"
                     /translation="MAEPIDEYFTATVAAMDSSAERGCSDDEPVRQGSSLTARVCLAL
                     FDVQLGSRHLDLAARWLRSQGRGFYTIGSSGHEGNAAVAAALRPTDPALLHYRSGAFF
                     LARGALVDGREPLRDVLLGLVASTDEPISGGRHKVFGRHDLRIIPQTSTIASHLPRAV
                     GVAFSIARAKKLGVSCAWPEDAVTVCSFGDASVNHSTAVGAINAALHSAYQGVPMPLL
                     FVCEDNGIGISVKTPGGWIEQTYGNRDGLAYFGADGCDLPATFDAAVAAAAWVRTRRR
                     PAFLHLRTVRLMAHAGSDYEPAYRKPDEIAADYDRDPLLRTAKLLVIEGVLTPEEVLD
                     RYEGKRAEVMELADEVAEAKQLDSAQAVMAPLTLGMADATAAAPASLAASVRSSDPDA
                     PMTVALGINRALHDILDRYPEAIVFGEDVARKGGVYGVTRGLLNRTSSARVFDTLLDE
                     QSILGLALGAGVSGLLPIPEIQYLAYLHNAADQIRGEGATLGFFSNRQYRNPMVVRVA
                     GYGYQKGFGGHFHNDNSIAAIRDIPGVVVASPARPDDAAAMLHTCAAAAKTSGAVCIF
                     LEPIALYHTRDLHEDGDEQWLATYPTAGVPVGRARTYGEGTDLTIITFANGLRMSLRV
                     ARRLEAKGIDARVVDLRWLAPLPVEDMLREAAVSGKALIVDETRRTGGVGEGVLAALL
                     EHGYTGVVERVASADSFIPLGDAALEVLLSEETIEAAAVKLAS"
     gene            2740..3939
                     /locus_tag="VGP27_24900"
     CDS             2740..3939
                     /locus_tag="VGP27_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854574.1"
                     /translation="MTSHAIRPLAGVRIVEISSFVAVPLAGMTLGQLGAEVVRVDPID
                     GAADYHRWPVTDDGTSIYWAGLNKGKRSVAADIRSPQGQDLVQRLIADSGVLITNVAG
                     RDWHSYDTLTRLRPDLIHVEVSGRADGGTGVDYTVNAGLGFPMVTGPSDLAAPVNHVL
                     PAWDVTCGIYAALSVVTALRHRDASGRGQRISIPLENVALATAGNLSFLTEVMVNGTS
                     RDRIGNSIYGQYGQQFTSIDGVSFMIVALTRRHFRDLTELTGTTEAVAALARTLDADF
                     TDEGQRYRHRDALSGLFAEWFAAHTAADVTEALSASSVLWERYRTFAEVAGDERVTAN
                     PLFTALDQPRIGEYLAPGLPVSIDGDYPQAVSAPALGDDTAGVLAEWLGLSAGDIDQL
                     TESGTVR"
     gene            3936..4751
                     /locus_tag="VGP27_24905"
     CDS             3936..4751
                     /locus_tag="VGP27_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEV7854575.1"
                     /translation="MSRLRKLLQLEPGADDGAWIGPASGPDGKRAFGGQFVAQSLAAA
                     CRTVDSDRQPTNMHLQFLRGGAAGDPVEYTVTPVFDGRTSAARRVDAHQAGRLLTTSS
                     VSFAVELPGPEHGHRERLPGDPDTLPESGPAGPAPSMPLDELDIRIADVITAGEFVRR
                     FWWRATVPIPDEPVIHTLVAAYVTDVYMIDPALQVHGHSMKARTHRSGTTDSSIWFHR
                     PVRANEWNLLETRSPAAARGRGVVTGSLLRADGVIAATLVQEGLIAEREPSPQ"
     gene            complement(4720..>5084)
                     /locus_tag="VGP27_24910"
     CDS             complement(4720..>5084)
                     /locus_tag="VGP27_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEV7854576.1"
                     /translation="STVWQRLAVTLVLAVSLHGYFDSTHREVLVLAGFLLLIWLPAIR
                     CPVWLTVAAGTVAEASLYTYLTHYQVYPLFEAHPLLGVVAAIVVGILLTRVLNAARTW
                     LRERSEHTATADSVPALR"
BASE COUNT          798 a         1744 c         1754 g          788 t
ORIGIN      
        1 cggcgaagcg gccaacgccg aactcgggct gctgctcggg ctgaccggac gcaccctggc
       61 gggtgaactc ggcgaccggc tcccggcgct gcgacgcgac cggctcgtgg tgctgggtcc
      121 gcgcgacgac gcgtggcggc agcggttcaa cgtcggcacg ctcgccgaca acggagtgtg
      181 gttggcgccg ctggccgaag tggccgacga tccggcaggc gccgggcgcc gtgcaatcga
      241 gcagttggcc gaacgggtcg accgatggtg gctgcacatc gatctcgacg tgctggatcc
      301 gacggtgttc ccggcgcagg gtctgcccga cttccccgat gagcccggcg gtctgacctg
      361 ggaccagctc accgacatgg tgatggcctt gttcaccaca ccggcgtcgt gcatcggggg
      421 cagcatcgcc atctacgatc cggaccagga tccgaatcgg accggcgctg cgcagatcgt
      481 ggcgttcatc cgtaacgtgg tggcgcgccc cacgatcagt cgctagcgca ggagacccgt
      541 ggccgaaccc atcgacgagt acttcaccgc caccgtcgcg gcgatggact catccgccga
      601 gcgcggctgt tccgacgacg aaccggtacg gcaggggtct tcgctgacgg cgcgagtgtg
      661 cttggcactg ttcgatgttc agctcggcag ccgtcatctc gacttggctg cacgctggct
      721 gcgctcgcag ggcaggggct tttacacgat cggatcatcc gggcacgagg gcaatgccgc
      781 tgttgcggcc gcactgcgtc ccaccgatcc ggcgctcctg cactaccggt cgggtgcctt
      841 cttccttgcc cgcggcgccc ttgtcgacgg cagggagccg ctgcgcgatg tgctcctcgg
      901 tctggtggcg tcgacggacg agccgatctc cggcggtcgg cacaaggtgt tcggccgtca
      961 cgaccttcgc atcattccgc agacctcgac catcgcatcg catctgccgc gcgcggtcgg
     1021 cgtcgcgttc tccatcgcgc gcgcaaagaa gctcggggtc tcatgcgcat ggccggagga
     1081 tgccgtcacg gtgtgcagct tcggtgacgc gtcggtcaac cattcgacgg cggtcggcgc
     1141 tatcaacgcc gcgctgcact cggcgtacca gggcgtgccg atgccgttgc tcttcgtctg
     1201 cgaggacaac ggaatcggca tcagcgtcaa gacacccggc ggttggatcg agcagaccta
     1261 cggaaaccgt gacggcctgg cgtatttcgg agccgacgga tgcgacctgc ccgcgacgtt
     1321 cgacgccgcg gtggcggccg cggcctgggt gcgcacgcgt cggcggcctg cgttcctgca
     1381 cctgcgaacg gttcgcctca tggcgcacgc cgggtccgac tacgaaccgg cctaccgcaa
     1441 gcccgacgaa atagccgccg attatgaccg ggatcccttg ttgcgcaccg cgaaactgtt
     1501 ggtcatcgag ggcgtgctga cgccggagga ggtgctggac cgctacgagg gcaagcgcgc
     1561 cgaggtgatg gagctggcgg acgaagtcgc cgaggccaag cagctcgaca gtgcgcaggc
     1621 ggtgatggcg cccctgacgc tcggcatggc ggacgcgacc gctgccgccc ccgcctcgct
     1681 ggccgcgtcg gtcaggtcca gcgacccgga cgcgccgatg accgttgctc tcggaatcaa
     1741 ccgcgcgctg cacgacatcc tcgaccgcta tccggaagcc atcgtcttcg gcgaggacgt
     1801 cgcgcgcaaa ggcggcgtct acggggtgac ccgcggactg ctcaaccgga ccagttcggc
     1861 gcgggtgttc gacacgctgc tcgacgaaca gtccatcctc gggctggcgc tcggcgccgg
     1921 tgtgtcgggg ctgcttccga taccggagat tcagtacctc gcctatctgc acaacgctgc
     1981 ggaccagatt cgcggtgagg gcgcgacgct ggggttcttc tccaatcggc agtaccgcaa
     2041 tccgatggtc gtccgggtcg cgggctacgg ctatcagaag ggcttcggcg ggcacttcca
     2101 caacgacaac tcgatcgccg cgatccggga catccccggc gtcgtcgtcg cctcacccgc
     2161 tcgtcccgac gacgccgcgg cgatgctgca cacctgcgcc gcggcggcga agacgtcggg
     2221 tgcggtgtgc atcttcctcg agcccatcgc cctgtatcac acccgcgacc tccacgagga
     2281 cggcgacgag cagtggctgg cgacctatcc caccgcgggc gttcctgtcg gcagggcccg
     2341 aacctacggc gagggaacgg atttgaccat catcaccttc gccaacgggc tgcggatgag
     2401 cctgcgcgtc gcccgcaggc tcgaggccaa gggtatcgac gcccgcgtcg tcgacctgcg
     2461 gtggctggcg ccgcttcccg tcgaggacat gcttcgcgag gcggccgtca gcggcaaggc
     2521 gctcatcgtc gacgagaccc ggcgaaccgg gggagtgggc gagggcgtgc tggccgcact
     2581 gctcgaacac ggctacaccg gcgtcgtcga gcgggtagcg agcgccgaca gcttcatccc
     2641 cctcggcgac gcggcgctcg aggtgctgct gagtgaggag acgatcgagg ccgcggccgt
     2701 caaactggcc agttagcgcg actgcttaca cttggccgcg tgaccagcca cgcgatccgt
     2761 ccactggccg gggtccggat cgtcgagatc tccagtttcg tcgcggtgcc gttggcgggt
     2821 atgacgctgg ggcagctcgg cgccgaggtg gttcgggtcg acccgatcga cggcgccgcc
     2881 gactaccacc gctggccggt caccgacgac gggacgagca tctactgggc gggtctgaac
     2941 aagggcaagc ggtcggtggc ggcggacatc cgctcgccgc aaggccagga cctcgtgcag
     3001 cgcctgatcg ccgatagcgg cgtgctgatc acgaatgtgg ccgggcgcga ctggcattcc
     3061 tacgacaccc tgaccaggct gcggcccgac ctcatccacg tcgaggtgtc cgggcgcgcc
     3121 gacggcggta cgggcgtgga ctacaccgtc aacgccggcc tcggattccc gatggtgacc
     3181 gggccttctg acctcgcggc tccggtgaac cacgtgttgc cggcgtggga tgtgacgtgc
     3241 ggcatctacg ctgcgctgtc ggtcgtcacc gcgctgcgcc accgcgacgc ctccgggcgg
     3301 ggccagcgga tatccatccc gctggagaac gtcgcgctgg cgaccgcggg caacctcagc
     3361 ttccttaccg aggtgatggt gaacggcacc tcacgcgatc ggatcggcaa ctcgatctac
     3421 ggccagtacg gccagcagtt caccagcatc gacggcgtgt cattcatgat cgtggcgctc
     3481 acccgccgcc acttccgcga cctgaccgaa ctcaccggga cgacggaagc ggttgccgcg
     3541 cttgcccgca cgctcgatgc cgatttcacc gacgagggcc agcggtaccg gcaccgcgac
     3601 gcgctgagcg ggctgttcgc cgagtggttc gccgcccaca ccgcagccga cgtcaccgaa
     3661 gcgctgtcgg cgtcatcggt tctgtgggag cggtaccgga cattcgcgga ggtcgccggt
     3721 gacgaacggg tgacggccaa cccgttgttc accgccctcg accaaccgcg aatcggcgaa
     3781 taccttgccc ctgggctgcc ggtgtcgatc gacggcgact acccgcaggc ggtgtcagcg
     3841 ccggcgctcg gcgacgacac cgcaggagtg ctcgcggaat ggttggggct cagcgcgggc
     3901 gacatcgacc aactcaccga gtcgggcacc gtcaggtgag ccggctgcgc aaactgctgc
     3961 agctcgaacc gggcgccgac gacggcgcgt ggatcgggcc ggccagcggc ccggacggca
     4021 agcgcgcgtt cggtggtcag ttcgtcgcgc agagcctggc cgccgcgtgc cgaaccgtcg
     4081 actccgaccg gcagcccacc aacatgcatt tgcagttcct gcgcggcggc gcggcgggcg
     4141 atcccgtcga gtacacggtc acgccggtgt tcgacggacg gacctccgcg gcccgccgag
     4201 tcgacgccca ccaggccgga cggctgctta cgacgtcgtc ggtgtcgttt gccgtggagt
     4261 tgcccgggcc cgaacacggt catcgggagc gcttgccggg cgatccggac acgctgccgg
     4321 aaagcgggcc cgcgggaccc gcaccgtcga tgccgttgga cgaactggac atcaggatcg
     4381 ccgacgtgat caccgctggc gagttcgtgc ggcgattctg gtggcgggca acggttccga
     4441 tacccgacga gccggtcatc cacaccctcg tcgcggcata cgtcaccgac gtgtacatga
     4501 tcgatccggc gctgcaggtg cacgggcatt cgatgaaggc ccgcacacac cgcagcggga
     4561 cgaccgattc gtcgatctgg ttccaccggc ccgtccgcgc gaacgagtgg aacctgttgg
     4621 aaacccgctc gcccgccgcc gcgcgcgggc gcggtgtggt gaccggaagc ctgctgcggg
     4681 ccgatggcgt catcgcggcg accctggtgc aggaagggct tatcgcagag cgggaaccga
     4741 gtccgcagta gccgtatgct cgctgcgttc gcgcagccac gtgcgcgccg cgttcagcac
     4801 cctggtgagc agtatgccga cgacgatcgc ggcgacgaca ccgagcagcg gatgcgcttc
     4861 gaacaacgga tagacctggt agtgcgtcag gtaggtgtac agcgaggctt ccgccaccgt
     4921 gcccgcggcg acggtcaacc agaccgggca gcggatcgca ggcagccaga tcagcagcag
     4981 gaatccggcc agcaccaaca cctctcgatg cgtgctgtcg aagtagccgt gcaggctcac
     5041 cgccaacacc agcgtcacgg cgaggcgctg ccatacggtc gacg
//
LOCUS       DASYLX010001064         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2160545,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001064 DASYLX010000000
VERSION     DASYLX010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160545"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..2206
                     /locus_tag="VGP27_24915"
     CDS             83..2206
                     /locus_tag="VGP27_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV7854577.1"
                     /translation="MSDGDNATPGRPRASDSNGDNQWLTRSPRPPRAAAPWERHSAAE
                     PEDTATPAPTGSHTDGVTVADLIAKVTGSAPADLPSRHAAPEPEPEPKPEPEPPVMRP
                     PAPPPQPSEPRSRQPRQLRPKPRPAGPSDPDTEVIPAVSHADEIPDLAALRRSRLERS
                     APEWVGEPSAEPIGPPKKRKSRRRVMLAGRSLAALFAVLALAMTGGAWQWQSSKNHML
                     NRISALDPGSRDIVDANAQYGDENFLIVGVDSRYGQNGDMGAGGTADAGGTRSDTIML
                     VNIPANRKRVVAVSFPRDLNIEPMKCEPWNPETREYGPLYDDQTGSYGPAEVYTETKL
                     NSAYAFGGPKCLVKVIQKLSGLSINRFMAVDFSGFSKMVDALGGVEVCSTTPLEDYEL
                     GTVLASAGRQTVDGHTALQYVRARQVTTETNGDYGRIKRQQLFLSSLLRSMISKEVFF
                     SLNKLNDVVNQFIDDSFVDNINTKDLVQLGQSVQGIAAGRITFITVPTTGYMDEWGNE
                     HLREDDTHAIFDAIINDDPLPEERNADNTPVPGTPESLTNQTPESTPSETPAPKVSAD
                     AGEMVDAVTTDPTSVTVQVSNSTGEDGLGATAATELQQHGFNVTTPDDYPGPLTSTTV
                     FFSPGNEEAAATVASAFTAPTIERVNGKGKTVQVVLGSDFATVSPPKPSGSSVQVHVL
                     HGTGSTPTQLPEDLSVTNAADTTCE"
     gene            2311..>2493
                     /locus_tag="VGP27_24920"
     CDS             2311..>2493
                     /locus_tag="VGP27_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulatory protein
                     PhoU"
                     /protein_id="HEV7854578.1"
                     /translation="MRTAYHEQLDALTQQLGEMCGLAGVAMERATQALLQADLVLAEQ
                     VITDHEQIAAMSAKAEE"
BASE COUNT          476 a          870 c          786 g          361 t
ORIGIN      
        1 gcttcaactc aggtggcggc agcgcagtgg ctgatgcgca ccgacgcgca cgctgctcat
       61 agggtcggag gccagtaggc acatgagtga cggcgataac gccactcctg gtcgccctcg
      121 cgcgtccgat tcgaacggcg ataaccagtg gcttacccga tctccccggc caccgcgagc
      181 cgccgcgccg tgggagcgcc actcggctgc tgagccggag gacaccgcca cccctgcgcc
      241 gaccggcagc cacaccgacg gtgtgacggt ggccgatctg atcgccaagg tcacgggctc
      301 cgcccctgca gatctgccga gccggcacgc tgcacccgag cccgaacccg aacccaaacc
      361 cgaacccgaa cctccggtga tgcgtccgcc cgcgccgccg cctcaaccct ccgagccgcg
      421 ctccagacag ccaaggcaac tgcggccgaa gccgcgaccc gcgggaccgt ccgacccgga
      481 caccgaagtc atccccgcgg tgtctcacgc cgacgagatt cccgatctgg cggcgttgcg
      541 ccgctcccgg ctcgaacgca gcgcgccgga gtgggtcggc gagccgtccg ccgagcccat
      601 cggcccgccg aagaagcgca aatcgcgccg gcgcgtcatg ctggcgggcc gctcccttgc
      661 cgccctgttc gccgtgctcg cgcttgcgat gaccggtggt gcgtggcagt ggcagtcgtc
      721 gaagaaccac atgctcaacc gcatttcggc actggatccg ggttcgcgcg acatcgtcga
      781 cgcgaacgcg cagtacggcg acgagaactt cctcattgtc ggtgtggaca gccgctacgg
      841 ccagaacggc gacatgggcg caggcggcac cgcggatgcc ggcggaacgc ggtcggacac
      901 catcatgctg gtcaacatcc cggccaatcg caaacgcgtt gtcgcggtgt cgtttccgcg
      961 cgacctcaac atcgagccga tgaagtgtga gccgtggaat cccgaaaccc gcgagtacgg
     1021 gccgttgtat gacgaccaga ccggcagcta cggacccgca gaggtgtaca ccgagacgaa
     1081 gctcaactcc gcctacgcgt tcggcggacc caagtgtctg gtcaaggtca tccagaagct
     1141 gtccggcctg tccatcaacc ggttcatggc cgtggacttc tccgggttct ccaagatggt
     1201 cgacgccctc ggcggggtcg aggtgtgcag cacgactccg ctggaggatt acgagctcgg
     1261 caccgtgctg gccagcgcgg gccggcagac cgtcgacggg cacaccgcgc tgcaatacgt
     1321 acgcgcccgc caggtgacca cggaaaccaa cggtgactac ggacgcatca agcgccagca
     1381 actgttcctc tcatcgttgt tgcgttcgat gatctccaaa gaggtcttct tctctctcaa
     1441 caagctcaac gacgtcgtca accagttcat cgacgacagc ttcgtcgaca acatcaacac
     1501 caaggacctg gtgcagctgg gccagtcggt acagggcatc gccgccggac ggatcacctt
     1561 catcaccgtc cccacgacgg gatacatgga cgagtggggt aacgagcatc tgcgcgaaga
     1621 cgacacccac gcgatcttcg acgcgatcat caacgacgat ccgctgcccg aggagcgcaa
     1681 cgcggacaac acaccggtgc cgggcacgcc ggagtcgctg accaaccaga cccctgagtc
     1741 gacgccgagc gaaacgcctg cacccaaggt gtccgccgac gccggcgaaa tggtcgacgc
     1801 cgtcaccacc gacccgacgt cggtgaccgt ccaggtgtcg aattcgacgg gcgaagacgg
     1861 gctgggagcg acggccgcaa cggaattgca gcagcacggc ttcaacgtga cgaccccgga
     1921 cgactatccg ggtccgctga cttcgacgac ggtgttcttc tcgccgggta acgaagaggc
     1981 cgcagccacg gtggcatccg ccttcaccgc gccgacgatc gagcgcgtca acggtaaggg
     2041 caagacggtg caggtggtgc tcggctccga tttcgccacg gtgagcccgc cgaagccgag
     2101 tggctcttcg gtgcaggtcc acgttcttca cggcaccggt agcaccccga cacagctgcc
     2161 ggaggacctt tcggtcacca acgccgccga caccacctgc gagtagcgcg acggcgatcg
     2221 ccgaggcggc ggcgtccatg cggggggatt cacccttcgt tcaccatgca catcgtgacg
     2281 attcactgga ccagccctta ggctgggcgt atgcgaactg cgtaccacga gcagctggac
     2341 gccctgaccc agcagctcgg cgagatgtgc gggctggcag gtgtcgcgat ggagcgagcg
     2401 acccaggccc tgctgcaggc cgacctcgtg ctggccgaac aggtgatcac cgaccacgag
     2461 cagattgccg cgatgagcgc caaggcggag gaa
//
LOCUS       DASYLX010001065         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1171560,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001065 DASYLX010000000
VERSION     DASYLX010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171560"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGP27_24925"
     CDS             <1..831
                     /locus_tag="VGP27_24925"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV7854579.1"
                     /translation="DAILLGAIGDPSVPSGVLERGLLLTLRFQLDHHVNLRPSRLYPG
                     VDSPLAGNPPIDFVVVREGTEGPYTGTGGVIRVGTPHEVATEVSLNTAFGVERVARYA
                     FARAAERRKHLTLVHKTNVLSYAGKLWSRVVDEIGREYPDVEVAYQHIDAATIHMVTD
                     PARFDVIVTDNLFGDIITDLAAAVSGGIGLAASGNIDATRTNPSMFEPVHGSAPDIAG
                     KGTADPTAAIMSVALLLSHVGEADAAARVDKAVEQHLATRGDATLSTSEVGERIIASL
                     "
     gene            840..1445
                     /locus_tag="VGP27_24930"
     CDS             840..1445
                     /locus_tag="VGP27_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854580.1"
                     /translation="MATLRRQCWLFAIGSTLFALATVPGMSAAAGAGLSNLLCFVGSW
                     FFTTAGWMQLVLARPRLGVGWQSAATQFVGTVLFNISTGSALWAHAVNPERRLVWAPD
                     MLGSLAFLVSGVLGIVAVTAIVGYIEFASRDWQAEWINMIGCLAFGVSALGAFVTETG
                     VTEDALLANAGTFVGALCFLAAALLVLPHWRKSPDPPGDRR"
     gene            complement(1535..1858)
                     /locus_tag="VGP27_24935"
     CDS             complement(1535..1858)
                     /locus_tag="VGP27_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV7854581.1"
                     /translation="MTTIRTDSTHATLINVFTVEPEKASDLAALLTSATENVMQHQLG
                     FRSANIHLSTDRTRVVNYAQWDSPQAYEKMLADATAREHMATAAAMAVSFDPHLYTVE
                     SVHER"
     gene            1920..2354
                     /locus_tag="VGP27_24940"
     CDS             1920..2354
                     /locus_tag="VGP27_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV7854582.1"
                     /translation="MGVSETEWAVGYLVKRVQQSLRRQCDVALRPSGLSMAQYAAMRA
                     LADHPEASASELARLCFVTRQSLQDLLAGLRSAGFVRDAKVPPRGRARSLEVTAAGAK
                     RLAQAHTAVMSVDSTMLQGVPESAQRQLAALLSRCANNLENS"
     gene            complement(2358..2789)
                     /locus_tag="VGP27_24945"
     CDS             complement(2358..2789)
                     /locus_tag="VGP27_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854583.1"
                     /translation="MWDEAAKWLNKFPEAVLTAIDRNGYPLSYRVSTGAYNKASGELP
                     VSPPPGPVDVADGPASLLCHSHDENLWNLQMITVKGALERRDGTWIFRTESFKPPSKL
                     AVLDFVRNMRRSAQKYLDKRGLARPEVNWSAIKDVQRRAKD"
     gene            complement(2779..3291)
                     /locus_tag="VGP27_24950"
     CDS             complement(2779..3291)
                     /locus_tag="VGP27_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7854584.1"
                     /translation="MTPSDAVDIIDRYFTCEFTTLSRNGSPITWPVSPRLLADGRFLL
                     TTSIGMPQKAFNIRRNPKVSMLFSEPTGSGVAQPGAVLIQGDATAQDRIVTDVSSDPD
                     LAALTETLTARQPAGAFWSTRVGRHLGWPYYMRLLIYVTPRRVLYWPTRDDFSTAPEE
                     LDANEARRVG"
     gene            complement(3351..3668)
                     /locus_tag="VGP27_24955"
     CDS             complement(3351..3668)
                     /locus_tag="VGP27_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEV7854585.1"
                     /translation="MRRIVGSAVWAAATLTTAAACSFSASTTPTVDKGELAKEISAQL
                     QKQVGRAPESVDCPDDLKGEVGATTRCSLTDAGETYGVNVDVTKVDGTDVKFDLKVDD
                     KPQ"
BASE COUNT          606 a         1206 c         1303 g          735 t
ORIGIN      
        1 gacgcgatcc tgttgggcgc catcggtgat ccctctgtgc ccagcggtgt gctcgagcgc
       61 ggcctgctgc tgacgttgcg gttccaactc gaccaccacg tgaaccttcg tccgtcgcgg
      121 ctgtatccgg gtgtcgacag cccgctggcc ggtaatccgc cgatcgactt cgtcgtcgtg
      181 cgagagggca ccgaaggccc gtacaccggc accggtggcg tcatccgcgt cggcacaccg
      241 cacgaggtgg ccaccgaggt cagcctgaac accgcgttcg gcgtcgagcg cgttgcccgt
      301 tacgcgttcg cacgtgcggc cgagcggcga aagcatctga cgctggtgca caagaccaac
      361 gtgctgtcgt atgccggcaa gctgtggtcg cgggtggtcg acgagatcgg ccgggagtat
      421 cccgatgtcg aggtcgctta tcaacacatc gacgccgcca ccatccatat ggtgaccgat
      481 cccgcccggt tcgacgtcat cgtcaccgac aacctgttcg gcgacatcat caccgatctg
      541 gccgccgcgg tgtccggcgg tatcgggctg gcggccagcg gcaatatcga tgccacacgg
      601 accaacccgt cgatgttcga accagtgcac ggcagtgcgc ccgacatcgc gggcaagggc
      661 acagccgatc ccaccgcggc gatcatgtcg gtggcgttgt tgttgtcgca tgtcggtgag
      721 gccgacgcag cggcgcgcgt ggacaaggcc gtcgagcagc atctggccac ccgcggcgac
      781 gcgacgttgt cgacctcgga ggtcggcgag cggatcatcg cgagcttgta accccgtcgg
      841 tggcgactct gcggcggcag tgctggttgt tcgcgatcgg ctcgacgttg ttcgcgctcg
      901 ccaccgtgcc ggggatgtcc gcggccgcgg gcgccgggct gagcaacctg ctgtgtttcg
      961 tcggttcctg gttcttcacc accgccggat ggatgcagct ggtgctggcc cgaccgagac
     1021 tgggcgtcgg gtggcagtcc gcggcaacgc agttcgtcgg cacggtgctg ttcaacatca
     1081 gcaccgggtc cgctttgtgg gcgcatgccg tcaacccgga gcggcgcctg gtgtgggcac
     1141 ccgacatgct cggctcgctg gcctttctgg tcagcggggt gctcggcatc gtcgcggtga
     1201 cagcgattgt cggctacatc gagttcgcat cccgtgactg gcaggcggag tggatcaaca
     1261 tgatcggctg cctcgccttc ggtgtctcgg cactcggcgc attcgtcacc gagacgggag
     1321 tgacggagga cgcgctgctg gccaacgcgg gcaccttcgt cggtgcgctg tgcttcctgg
     1381 ccgccgcgct cctcgtgctg ccgcattggc ggaaatcgcc tgatccaccg ggggataggc
     1441 gataggccgc ggctatcctc ggactattcg gagcgcgcgg cgtggcggcg tatcgccgac
     1501 gccttcagcg gcgcatcggc gatgtcccgt tggactaacg ctcgtgcacc gattccacgg
     1561 tgtacaggtg gggatcgaag ctgacggcca tcgcggccgc ggtggccatg tgttcgcggg
     1621 cggtcgcgtc ggcgagcatc ttctcgtagg cctgcggact gtcccattgc gcgtagttga
     1681 cgacccgggt gcggtcggtg ctcagatgaa tgttcgccga tcggaagccg agctgatgtt
     1741 gcatcacgtt ctcggtggcc gacgtcaaca gcgcggccag atctgaggcc ttctccggtt
     1801 cgacggtgaa cacgttgatc agggtggcgt gggtgctgtc ggtgcggatg gtcgtcatga
     1861 tgcctccgtt gtcaggttcc ttacataggc aaccatagca ctgatgtaag gttcctgtca
     1921 tgggcgtttc cgaaaccgag tgggccgtcg gctacctggt gaaacgtgtg caacagtcac
     1981 tgcgcaggca atgcgatgtt gccttgcggc cgagcgggct ctcgatggct cagtacgcgg
     2041 cgatgcgggc gctggccgat catccggagg cctcggcctc ggagttggcg cgactgtgtt
     2101 tcgtgaccag gcagtcgctt caagacctgc ttgccggcct gagatccgcc ggcttcgtca
     2161 gggacgccaa agtaccgcca cgcggtcgcg cgcgttcgct ggaggtcacc gccgcaggcg
     2221 cgaaacgcct tgcgcaggcc cacaccgccg tgatgagcgt ggattcgacg atgctccaag
     2281 gagttccgga gagtgctcaa cgccagctgg ctgcccttct gagtcgctgc gcgaacaacc
     2341 tcgaaaacag ctgacggtca gtccttcgcg cggcgttgaa cgtctttgat cgcagaccaa
     2401 ttcacctcag ggcgcgcgag tccgcgcttg tcgagatatt tctgcgcaga ccgccgcatg
     2461 ttcctgacga agtccagcac agccaacttc gatggcggct tgaagctttc tgtccggaag
     2521 atccatgtcc cgtcgcgcct ctcgagggcc cctttgaccg tgatcatctg cagattccac
     2581 aggttctcgt catgcgagtg gcacaacaga cttgctgggc cgtcggcgac atcgaccgga
     2641 ccagggggcg gcgacacggg caattcaccg gacgccttgt tgtacgcgcc agtcgacacc
     2701 cgataactga gggggtagcc gttgcggtcg atcgcagtga ggaccgcctc gggaaacttg
     2761 ttgagccact tggccgcttc atcccacacg gcgcgcctcg ttggcgtcga gttcctcagg
     2821 agcggtggag aagtcgtcgc gggtgggcca atacagaaca cgtcgcggag tcacgtagat
     2881 gaggaggcgc atgtagtagg gccaccccag gtgtctgccg acccgggtgc tccagaaggc
     2941 gccggccggt tggcgtgcgg tcagcgtttc ggtgagcgca gcgaggtcgg gatccgacga
     3001 gacgtcggtc acgatgcggt cctgggccgt tgcgtctccc tggatgagta cggcacccgg
     3061 ttgggcgacg ccgctgccgg ttggctccga gaagagcatg ctgactttcg gattacgccg
     3121 gatgttgaac gctttctgcg gcatcccgat gctcgtcgtc agcaggaaac ggccgtcggc
     3181 gagcagtcgc ggcgacaccg gccaggtgat cggtgaaccg ttgcgcgaca gcgtggtgaa
     3241 ctcgcatgtg aagtagcggt cgatgatgtc gaccgcatcg gaaggcgtca cgagcggagc
     3301 atatgcccgg cgcggacgtg cattcgccga tccgcagttt tccgtgcccg ctattgcggt
     3361 ttgtcgtcga ccttgaggtc gaacttgacg tcggtgccgt cgaccttggt cacgtcgacg
     3421 ttgacgccgt aggtctcgcc ggcgtcggtg agcgagcagc gggtcgtcgc accgacttcg
     3481 cccttgaggt cgtcgggaca gtcgacggac tccggcgcac gcccgacctg cttctggagt
     3541 tgcgccgaga tctctttcgc gagttcaccc ttgtccaccg tcggcgttgt cgaagcggag
     3601 aacgaacaag ccgccgcggt ggtcagcgtc gcagctgccc acaccgcgga tccgactatt
     3661 cgccgcatcg ggaacctcct gtgtgtgcgg ttgcgcagca actctggcac acaccacggg
     3721 gccgtgcgcc gctttccatc atgatcacgt gcgaccgctg ttcagcgggt ccgtttcgac
     3781 ggcgaccagc ggtatcgcga tcagcgggaa gaaggcacag acggcaaacg ccgctgcata
     3841 gcccgcaacg
//
LOCUS       DASYLX010001066         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1938925,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001066 DASYLX010000000
VERSION     DASYLX010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938925"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGP27_24960"
     CDS             <1..222
                     /locus_tag="VGP27_24960"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV7854586.1"
                     /translation="ALRRSIKFPAFLDGIDAVRRVGEHAEQKDHHPDIDIRWRTVTFA
                     LVTHSEGGITDKDVEMAREIDGILEGSAQ"
     gene            complement(158..1423)
                     /locus_tag="VGP27_24965"
     CDS             complement(158..1423)
                     /locus_tag="VGP27_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HEV7854587.1"
                     /translation="MTRVGRIEATPAKTVARLPARLAAAAPWLLLISIAVRLAWTYLV
                     PNGANFVDLHVYVGGAATLDHAGTLYDYVYADQTPDFPLPFTYPPFAAVVFYPLHLLP
                     FGLVAFAWQLGIVAALYGVVRISQRLLPVTDEFATSRRVAMLWTAVGIWLEPLRSTFD
                     YGQINVLLVLAVLYAVYNTRWWISGLLVGLAAGVKLTPAVSGLYFLGARRWGTVVFSA
                     VVFLATIGVSALVVGDQARYYFTELLGDASRVGPIGTSFNQSWRGGISRILGHDAGYG
                     PIVVAALVITAVLAFLAWRATTGDDRLGRIVVVQLFGLLLSPISWTHHWVWVLPLMIW
                     LLHGPLRRLRGARILGWGWLALTIVGVPWLLSFAQPTIWEISRPWYLAWAGLIYIVAT
                     LVTLAWVASTGPTLLRSRQSPEPSPHPCP"
     gene            1545..2180
                     /locus_tag="VGP27_24970"
     CDS             1545..2180
                     /locus_tag="VGP27_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854588.1"
                     /translation="MNRTRKLFVSSIIAAAGAVSALSLSATAAADPVPPVPVDPGSQL
                     LAQLGTVGSAAPMLMQTLAKAMSGTAAPAAAPATPATPPPGSATASLTLPEVPTLPAS
                     AAQAAPAAAPAAAPAAAAPANALSSLTSALPAPLSNTGGLAQLLPSGLSLPGLTAPAA
                     PATAATAPLATAPALAAPLAAPVAAAPAAAAPAAAAALPAFPSASVLSALP"
     gene            2222..3166
                     /locus_tag="VGP27_24975"
     CDS             2222..3166
                     /locus_tag="VGP27_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854589.1"
                     /translation="MSATRKLLANAAVAVGASTALLLGMTGTANAEPVAPLPIDATQA
                     PGMSAMQDLSPAIQKAAADPYNAVSTLMAAAAAFTGNSAAPTDSKNMASAVNSFVAQP
                     AAAHVPAPGAAPGAEAHLPAGVDPSHAVGPAPDALAAPAPDAAMVTDAAPAPEAAPAP
                     GPDGAPAPEAAANPQFGPDAPTTQDFLYPSISNSCLKDGGNVLATAISVAGPAKIPAP
                     GPAAGQTAYVFTAIGTPGPAAEQKLPLNVTWVNLTTGKSGSATLKPRSDINPQGPTTL
                     SVIADTGSGSIMSTIFGQVTTTEKQCQFMPTIGSTVVP"
     gene            complement(3173..3319)
                     /locus_tag="VGP27_24980"
     CDS             complement(3173..3319)
                     /locus_tag="VGP27_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEV7854590.1"
                     /translation="MKTHLNCPCGESITGSDEDDLVEKAQKHLSEQHPGREYDRDAIL
                     FMAY"
     gene            3352..>3614
                     /locus_tag="VGP27_24985"
     CDS             3352..>3614
                     /locus_tag="VGP27_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV7854591.1"
                     /translation="MDIYDVMRTTGAVREYTGDPLSDQVLERILDNARFAPSGGNRQG
                     NHVIVVRDPKTREDLIDCTIPGAKRYIAQLRNGEGPWNPLRPCG"
BASE COUNT          657 a         1305 c         1115 g          537 t
ORIGIN      
        1 gcgttacgcc ggtcgatcaa gttccccgcg ttcctcgacg gcattgacgc cgtgcgccgc
       61 gtgggcgaac acgccgagca gaaggatcat catcccgata tcgatatccg ctggcggaca
      121 gtgactttcg cgctcgtcac gcattccgag ggcggcatca cggacaagga tgtggagatg
      181 gctcgggaga ttgacgggat cttagaaggg tcggcccagt agacgcgacc cacgccagcg
      241 tcaccaacgt cgccacaatg tagatcaatc ctgcccaggc cagataccag ggcctgctga
      301 tctcccagat cgtgggttgg gcgaagctga gcaaccacgg cacgccgacg atcgtcagcg
      361 ccagccagcc ccagccgagg atgcgcgcac cacgcaggcg tcgcagcggg ccgtgcagca
      421 accagatcat caggggcagc acccagaccc agtggtgtgt ccacgaaatc ggagacagca
      481 gcagtccgaa cagctgtacc accacgatcc ggcccagccg gtcatcgccg gtcgtcgccc
      541 gccacgccaa aaacgccaac actgcggtga tgacgagcgc cgccaccacg atcggcccgt
      601 agccggcatc gtgaccgagg atccgcgaga tgccgccgcg ccacgactgg ttgaacgacg
      661 ttccgatcgg ccccaccctg ctggcatcac cgagcagttc ggtgaagtag taacgggctt
      721 ggtccccgac caccaaggct gacactccga tcgtcgccaa aaacacgact gcagagaaca
      781 ccacggttcc ccagcgccgc gcccctagaa agtagagccc gctgaccgcc ggcgtcagct
      841 tcactcccgc cgcgagcccg accagcaacc cggagatcca ccacctcgtg ttgtagacgg
      901 cgtacagcac agccaatacc aacagcacat tgatctggcc gtagtcgaac gtgctacgca
      961 gcggttcgag ccagataccg acggcggtcc acagcatcgc gacgcgccgg ctcgtcgcga
     1021 actcgtcggt gacgggcagc aaccgctggc tgatgcgcac cacgccgtaa agcgccgcga
     1081 cgatgcccag ctgccacgcg aaggcgacga gcccgaacgg caacagatgc agcggataga
     1141 acacgacggc ggcgaacggc ggataggtga acggcagcgg gaagtccggc gtctgatcgg
     1201 cgtacacgta gtcgtacagc gttcccgcgt ggtccaaggt ggctgcaccg ccgacgtaca
     1261 cgtgcaggtc gacgaagttc gcgccgttcg gcaccaaata cgtccacgcc agccgcacgg
     1321 cgatgctgat caacagcagc catggcgcgg cagccgccaa tcgcgctggc agccgcgcga
     1381 ctgtcttcgc gggtgtggct tctattcgac cgactctagt gatcggtaac cgattgccct
     1441 cgtctcaata acggttgcat aaatgccaca cgtgtcactt gagtcacagc agtaaccaac
     1501 tagcttcggg ttcaggcctt cgccgtccaa cgattgggag aaccatgaac cgcactcgga
     1561 aactgtttgt ctcgtccata atcgccgccg cgggcgcggt ctctgcgctg tcgctcagcg
     1621 cgaccgcggc cgcagatccg gttccgccgg tcccggtgga ccccggctcc caactactcg
     1681 cccagctcgg gaccgtcggt tcagccgcgc cgatgttgat gcagaccctc gcgaaggcca
     1741 tgtccggcac cgcggcaccg gccgctgctc ctgcgacgcc tgcaacgcct cctcctggca
     1801 gcgccaccgc atcgctgacg ctgcccgagg tgcccacgct tccggcctcc gctgcgcagg
     1861 ccgctcccgc tgccgctccc gctgccgcac ctgcggccgc cgctccggcc aacgcgcttt
     1921 cgtcgctgac ctcggcgctt ccggcaccgc tatccaacac cggaggactg gcccagttgc
     1981 ttccgagcgg actttcgctg ccgggtctga ccgcgcctgc ggcacctgct acggccgcca
     2041 ccgcacccct cgcgacggcg cccgcgttgg ccgcacccct ggcagcaccg gtcgcggcgg
     2101 cgcccgcggc tgcggcacct gcggcggcgg cggcccttcc ggccttcccg tccgcgtcgg
     2161 tgctcagcgc cttgccgtga tctgaaaacc cagcccaccc cttcgaaagt ttgggagaac
     2221 catgtcagcc accaggaagc tgctcgccaa cgccgccgtc gcagtcggcg cgtcaacggc
     2281 actgctgctg ggaatgacgg gtaccgcgaa cgccgagccg gtcgcaccgc tgccgatcga
     2341 tgccacgcag gcaccgggca tgtcagcgat gcaggacctg agcccggcga ttcagaaggc
     2401 ggccgccgat ccgtacaacg ccgtgtcgac gctgatggcg gccgcggcgg cgttcaccgg
     2461 taactcggcg gctcccacgg actcaaaaaa catggcgtcg gctgtcaaca gtttcgtcgc
     2521 ccagcccgct gcggcgcatg tgcccgcgcc gggcgcggcg ccgggcgcgg aggcgcatct
     2581 gcctgccggc gtggatccct cccatgccgt cgggccggcg ccagacgccc ttgctgcccc
     2641 cgctccggac gcggcaatgg ttaccgatgc ggctccggca ccggaggcgg cgcccgcgcc
     2701 gggtccggac ggcgcgccgg cccccgaggc cgccgcgaat ccgcagttcg gtcccgacgc
     2761 gccgacgacg caggacttcc tgtacccgtc gatcagcaac agttgcctga aggacggtgg
     2821 aaatgttctg gccaccgcga tctcggtggc cgggcccgcc aagattccgg cgccaggacc
     2881 cgcagcgggt caaacggcgt acgtattcac cgcgatcggc accccgggcc cggcggccga
     2941 gcagaaattg ccactgaacg tcacgtgggt caacctcacc accggcaagt caggtagcgc
     3001 caccctcaag ccgcgctccg acatcaatcc ccagggaccg acgacgctct cggtcatcgc
     3061 cgacacgggc tcgggcagca tcatgtcgac gatcttcggc caggtgacca cgaccgagaa
     3121 gcagtgccag ttcatgccca ccatcggctc gacggtggtg ccctgagtta cctcagtagg
     3181 ccatgaagag gatggcgtcc cggtcgtatt cgcggccggg atgctgctcg gaaagatgct
     3241 tttgcgcctt ctcgacgaga tcgtcctcgt ccgaaccggt gatggactcc ccgcagggac
     3301 agttcaagtg cgtcttcatc tctctacgat cccacagtca agatggacgg catggacata
     3361 tacgacgtca tgcgcaccac gggtgcggtt cgcgagtaca ccggcgaccc tttgtccgac
     3421 caggtcctcg aacgcatcct cgacaacgcg cggttcgcgc cgagcggtgg caaccggcag
     3481 ggcaaccacg tcatcgtcgt ccgggacccg aagacacgcg aggatctcat cgactgcacg
     3541 atccccggag ccaagcgcta tatcgctcag ctgcgcaacg gggaggggcc gtggaacccg
     3601 ttgcggccgt gcgg
//
LOCUS       DASYLX010001067         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1939083,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001067 DASYLX010000000
VERSION     DASYLX010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939083"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..861)
                     /gene="lmeA"
                     /locus_tag="VGP27_24990"
     CDS             complement(46..861)
                     /gene="lmeA"
                     /locus_tag="VGP27_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan chain length control protein LmeA"
                     /protein_id="HEV7854592.1"
                     /translation="MGQNVRVRKLVIGAISAVIAVVVAAVGVDFGAAIYAEYRLARAV
                     RDEAKLTFDPWVGIIGFPFITQARDRHYDQIEIRANGVDHPVAGKVSYEATLYSIDVP
                     TDSWLLDASTELPVGKVESRTIIDSTHLGRFMGIPDLLVETPLKEANTATGGTTESGI
                     SGNKGLVFTGTPTAAGFDKRVSVSVDLSVAGPDQTTLVFTATGVLTGAGTADQQVPED
                     KTPAVLAAFTTTLPGLKLPFGIAPTTEGARGSDVIVEGIAEGVTITLDEFTQS"
     gene            1012..1788
                     /locus_tag="VGP27_24995"
     CDS             1012..1788
                     /locus_tag="VGP27_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7854593.1"
                     /translation="MDLLLLTADPHPESVLPSLSLLAHNVRTAPTEVASLLEAGSADV
                     AIVDARTDLAAARGLCRLLGTTGTSAPVVAVVNEGGLVAVNMEWGLDEILLPSTGPAE
                     IDARLRLLVGRRGGAASQENAGKVSLGELVIDEGTYTARLRGRPLDLTYKEFELLKYL
                     AQHAGRVFTRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGTEYESLIGTVRNVGYKA
                     VRPARGRPTAAGSDAPDDLEDAYDAPVGLPDELADPLRTQ"
     gene            1785..2705
                     /gene="mshD"
                     /locus_tag="VGP27_25000"
     CDS             1785..2705
                     /gene="mshD"
                     /locus_tag="VGP27_25000"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEV7854594.1"
                     /translation="MTPLSWRTDLTDEDQRAIRALIAEAKKEDGIAPVGDQVLRELSQ
                     HRTRHLLALDGDTVVGYLDLAPATDDAPAMAELVVHPQWRRRGIGSAMARTALADGGS
                     GTRIWAHGNLPPARATAKALGLTAVRELLQMRRPLPGAGWQATSNSALPPLKIPAGVR
                     IDTYRGPDDDAELLRVNNAAFAWHPEQGGWTAADVAERRGEPWFDAEGLFMAFDEETG
                     ALLGFHWTKVHSEDLGEVYVVGVDPAAQGRGIGATLTLIGLHHLAKRLSTNSQPTVIL
                     YVEADNSAAVNTYRRLGFEVSGIDVAYAAD"
     gene            2838..>3354
                     /locus_tag="VGP27_25005"
     CDS             2838..>3354
                     /locus_tag="VGP27_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7854595.1"
                     /translation="MKLNSIGKTFGTTLSVAALTALTLSGCGSDNNAGTSTSSGASGT
                     AASSADCGGKNSLTAEGSTAQQNAIAEFNKVWGQVCSGKTLSYNPTGSGAGVDQFLAK
                     QVDFAGSDSALSAEQGPKAAERCAGNPAWNLPLVFGPVAMAFNVEGADKLVVNGDVLA
                     KIFQGQITKWND"
BASE COUNT          571 a         1117 c         1118 g          548 t
ORIGIN      
        1 cgaacgccgc tatgagcacg gcgatgaccg cgacaactga agtgctcatg actgtgtgaa
       61 ctcatccagc gtgatggtta ctccctcggc gatgccctcg acgataacgt cggatccgcg
      121 ggcgccttcg gtggtcggag cgatgccgaa gggcaacttc agcccgggca gggtggtggt
      181 gaacgcggcg agcacggccg gcgtcttgtc ctcgggcacc tgctggtccg cggtgcccgc
      241 cccggtgagc acgcccgtcg cggtgaatac gagagtcgtc tgatccgggc ccgcgacgga
      301 gaggtccacc gaaacgctga ccctcttgtc gaagccggcc gcggtgggcg tgccggtgaa
      361 caccagccct ttgttgcccg agatgcctga ttccgtcgtg ccgccggtgg cggtgttggc
      421 ttccttgagc ggcgtctcca ccagcaggtc ggggatgccc atgaagcggc ccaggtgggt
      481 ggagtcgatg atcgtgcggc tctcgacctt gcctaccggc agttccgtac tcgcatcgag
      541 cagccaggaa tccgtcggga cgtcgatcga atacagggtg gcctcatagg acaccttgcc
      601 cgcaaccggg tgatccacgc cattcgcacg gatctcgatc tggtcgtaat gccggtctct
      661 ggcctgcgtg atgaacggaa agccgatgat gccgacccat gggtcgaatg tcagcttcgc
      721 ttcgtcccga accgctctgg ccagccgata ttcggcgtag atggccgccc cgaagtcgac
      781 gccgaccgcc gcgaccacta ccgcgatcac agcggatatc gccccgatca ccagcttgcg
      841 cacccggaca ttctggccca ccggagcacg gcaccctgct cggcgcggct aatcggcagg
      901 tatcgggtta tcgttagtcc accggtaacg cccagatgtc cactacgaat ctgcgaggta
      961 tccacagaca gcgatgtccg tcaatatctg cccggcacgc tggagggccc gttggatcta
     1021 ctgctgttaa ccgcagaccc gcatcctgag tcggtgctgc cctcgttgtc gctgctcgct
     1081 cataacgtgc gcacagcgcc caccgaggtg gcctcgttgc tggaggcagg cagcgcagac
     1141 gtcgcgatcg tcgacgcgcg cacggacctg gcggccgccc gtggactgtg ccggctgctc
     1201 ggcacgaccg gcacttccgc tcccgtcgtc gctgtcgtca acgagggcgg cctggtagcg
     1261 gtcaacatgg aatgggggct cgacgagatt ctgctgccga gcaccgggcc cgccgagatc
     1321 gacgcacggc tgcgcctcct cgtgggccgc cgcggcggcg ccgcgagcca ggagaacgcg
     1381 ggcaaggtaa gcctgggcga gctcgtcatt gacgaaggca cgtacacggc tcggctgcgc
     1441 ggccggccgc ttgacctcac ctacaaggaa ttcgaactgc tgaagtatct ggcccagcac
     1501 gccggccggg tgttcacccg cgcccaactt ctgcaagagg tgtggggcta cgacttcttc
     1561 ggcggcaccc gcacagtcga cgtccacgtc cgtcggctgc gcgccaagct cggcaccgaa
     1621 tacgagtcgc tcatcggcac tgtgcgcaac gtcggataca aggcggtgcg tccggccagg
     1681 ggacgcccaa ccgctgccgg ttccgatgca cccgatgacc tcgaggacgc gtacgacgcg
     1741 cccgtcggac tgccggacga gctggctgat ccactgcgca ctcagtgacg ccgttgtcct
     1801 ggcgcaccga cctcaccgac gaggaccagc gcgccatccg ggcgctgatc gccgaggcga
     1861 agaaggaaga cggcatcgcc ccggtcggtg accaagtgct gcgcgagctt tcccagcacc
     1921 gcacccggca cctgctcgcg ctcgacggtg acaccgtcgt ggggtacctg gatctggcac
     1981 cggcaaccga tgacgccccg gcgatggccg agctcgtcgt ccatccgcag tggcgcaggc
     2041 gcggcatcgg atcagcgatg gcccgaacgg ccttggccga cggcggcagc ggtacccgga
     2101 tctgggcgca cggcaacctg ccgcccgcac gggccaccgc gaaggcgctg gggctgaccg
     2161 ccgttcgtga actgctgcag atgcgcaggc cgttgccggg agccggttgg caggcgacat
     2221 cgaactcagc tctgccgccc ttgaaaatcc cggcaggggt gcggatcgac acctaccgcg
     2281 gcccggatga cgacgccgaa ctgctccgtg tcaacaacgc cgcattcgcc tggcacccag
     2341 agcagggcgg ctggacggcc gccgacgtcg ccgagcgccg tggcgagccc tggttcgatg
     2401 ccgagggctt gttcatggcg ttcgacgagg agaccggtgc gctgctcggg ttccactgga
     2461 cgaaggtgca cagcgaggat ctcggcgagg tgtatgtggt gggcgtcgac ccggccgcgc
     2521 agggccgcgg tatcggcgcg accctgacgc tgatcgggct gcaccatctg gcgaagcggc
     2581 tgtcaacaaa ctcccagcca acggtgatcc tttatgtcga ggcagataac tcggcggcgg
     2641 tgaacaccta tcgacggctg ggtttcgagg tgtccggcat cgacgtggcc tacgccgccg
     2701 actagccgtg cgagctgcaa attagctgaa cctgttcacc gttggttcac cttccatccg
     2761 tcgggtgtcc actggggccg catacgttgc cggagagtct gaagccgtca agagtcctga
     2821 agcgaaagtg ggtttatgtg aagctcaaca gcattggcaa gacgttcggt acgaccctct
     2881 cggtcgcggc gctcaccgcg ctgacgctgt ccgggtgcgg cagcgacaac aacgcaggaa
     2941 cgagcacctc ctccggtgcg tcagggaccg ccgcgtcatc cgccgactgc ggcggcaaga
     3001 actcactcac cgctgagggc tcgacggctc agcagaacgc gatcgcggag ttcaacaagg
     3061 tctgggggca ggtctgctcg ggcaagacgc tgtcgtacaa cccgaccggt tcgggcgcgg
     3121 gcgtggacca gttcctggcc aagcaggtcg acttcgcagg ctccgactcg gcgctgagcg
     3181 ccgagcaggg cccgaaggcc gccgagcgct gcgccggcaa cccggcgtgg aaccttcctt
     3241 tggtgttcgg cccggtggcg atggccttca acgtcgaggg tgccgacaag ctcgtcgtca
     3301 acggcgacgt gctggccaag atcttccagg gccagatcac taagtggaac gacc
//
LOCUS       DASYLX010001068         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2380366,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001068 DASYLX010000000
VERSION     DASYLX010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380366"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGP27_25010"
     CDS             complement(<1..293)
                     /locus_tag="VGP27_25010"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV7854596.1"
                     /translation="MTARVGVITFPGTLDDIDAARAVKLAGAEAVSLWHADADLKDVD
                     AVIVPGGFSYGDYLRCGAIAKFAPVMKSVIDAATSGMPVLGICNGFQVLCEAGL"
     gene            complement(290..529)
                     /gene="purS"
                     /locus_tag="VGP27_25015"
     CDS             complement(290..529)
                     /gene="purS"
                     /locus_tag="VGP27_25015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV7854597.1"
                     /translation="MAKVVVHVMPKAEILDPQGQAIVGALGRLGYNGISDVRQGKRFE
                     LEVDDSIADETVAEIAESLLANIVIEDWSVSREPS"
     gene            complement(565..1242)
                     /locus_tag="VGP27_25020"
     CDS             complement(565..1242)
                     /locus_tag="VGP27_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEV7854598.1"
                     /translation="MRLMLAALLAAGGLALGPGVLGPAHAVPMNCPPNCDRIPNSAWI
                     APTALPLFSTYRWPGLPGVAVTATDPRFRFEQACASPPVPDDARSYAVAARAAVPQPE
                     GQWQLQVQVIHWRGETWRGGQLASAVFDKAVAALRGCQLTSPLTSPSLTTDSTNRMAA
                     VVSGVLPAQGVLHEYLLVNPYNSTIVETAMWTTTPPRVDWPSVPDAQMLDAMNAPLCT
                     AYIDSCR"
     gene            1274..1924
                     /locus_tag="VGP27_25025"
     CDS             1274..1924
                     /locus_tag="VGP27_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7854599.1"
                     /translation="MQLTHFGHSCLLAEFKDDSATTTVLFDPGNFSHGFEGITGLDAI
                     LITHQHPDHADTQRLPALLDANPQAALYADPQTAEQLGGPWRAVNVGDEFTVGGLTVR
                     GVGGKHAVIHPEIPVIDNISYLIGDGDHPARLMHPGDALFTPGEPVDVLATPAAAPWM
                     KLSEAVDYLRAVAPARAVPIHQAIINPNARGIYYGRLSEMTDTDFRVLDPENATTF"
     gene            complement(1937..>3313)
                     /locus_tag="VGP27_25030"
     CDS             complement(1937..>3313)
                     /locus_tag="VGP27_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEV7854600.1"
                     /translation="RQWAHAYVDFAAGEKRSFLRERGLTTFPLVGWAERGGYDARGHG
                     NSVPRFHITWGTGPAIVDVFARRLAGNDRVTYAHRHRVDELIVEDGSVVGVRGAVLEP
                     SDAARGVASSRNTFGDFEFRAQAVIVASGGIGGNHDLVRKNWPKRMGRVPEQLLSGVP
                     AHVDGRMIGITESAGAHVINSDRMWHYTEGITNYDPIWPLHGIRILPAPSSLWLDASG
                     KRLPAPLYPGFDTLGTLEHIARTGWDYTWFVLNARIIAKEFALSGQEQNPDLTGRSVR
                     DVLARVRPGAPAPVQAFVDKGVDFVSAKTLGELVSAMNTVPGVIPLDYATVEAEVTAR
                     DREVANRFTKDTQVTAIRAARGYLGDRFTRVVAPHRLTDPKAGPMIAVKLHILTRKSL
                     GGLETDLDSRVLRHDGGVFTGLYAAGEAAGFGGGGVHGYRSLEGTFLGGCIFSGRAAG
                     RAAARDTG"
BASE COUNT          543 a         1167 c         1081 g          522 t
ORIGIN      
        1 agcccggctt cacacagcac ctgaaatccg ttgcagatac ccaacaccgg catcccgctg
       61 gtcgcggcgt cgatgacgga cttcatcacc ggggcgaact tggcgatcgc accacagcgc
      121 aggtagtcgc cgtaggagaa gccgccgggg acgatgacgg cgtcgacatc cttgaggtcg
      181 gcgtcggcgt gccagaggct caccgcctcc gcgcccgcga gtttgaccgc ccgtgcggcg
      241 tcgatgtcgt cgagtgtgcc gggaaatgtg atgaccccca cacgcgctgt catgatggct
      301 ccctgctgac cgaccagtct tcgatcacga tgttcgccag gagcgattcg gcgatttcgg
      361 cgacggtctc gtcggcgatg ctgtcgtcga cctcgagctc aaatcgcttg ccctgcctga
      421 catctgatat tccgttgtag ccgaggcggc caagcgcgcc gacaatcgcc tgtccctgag
      481 ggtcgaggat ctccgctttc ggcattacgt gtacgaccac ctttgccacg agctcactct
      541 accggcggcg tcggctcgac cgcctcaccg gcacgaatcg atatacgccg tgcacagcgg
      601 ggcgttcatg gcgtcgagca tttgcgcatc gggcaccgac ggccagtcga cgcgcggcgg
      661 cgttgtcgtc cacatcgccg tctcgacgat ggtgctgttg tacgggttga ccagtaagta
      721 ctcgtgcagc accccctgtg cgggcaggac cccgctgacc actgcggcca tccggttggt
      781 gctgtctgtg gtgagcgacg gagaggtcag cggcgaggtc agctggcatc cgcgcaatgc
      841 ggcgaccgcc ttgtcgaaca cggccgacgc cagctggcca ccgcgccagg tctcaccccg
      901 ccaatggatc acttggacct ggagctgcca ctgcccttcc ggctgcggaa cggcggcccg
      961 cgccgcgacc gcgtagctgc gggcatcgtc ggggacggga ggtgaggcgc acgcctgctc
     1021 gaaccggaag cgggggtcgg tcgcggtgac cgcgaccccg ggcagacccg gccaccggta
     1081 cgtcgagaac agcggcagcg ccgtcggggc gatccacgca gaattcggga tccggtcaca
     1141 gttcggcggg caattcatcg gaaccgcatg ggcagggccg aggacgcccg gcccgagcgc
     1201 cagccccccg gccgccagta gtgccgccag catcagtcgc attgcccacc acccctgagc
     1261 gcacaatata ggcatgcagt tgacgcattt cggccattcg tgcctactcg ccgagttcaa
     1321 ggatgattcc gctacgacga cggtgctgtt cgaccccgga aatttctcgc acggcttcga
     1381 gggcatcacc gggctggacg cgatcctgat cacgcatcag caccctgatc acgccgacac
     1441 ccagcggctg cccgcgctgc tggacgccaa cccgcaggcg gcgctgtacg ccgacccgca
     1501 gaccgccgag cagctgggcg ggccttggcg ggccgtcaac gtcggtgacg agttcaccgt
     1561 cggcggcttg accgtccgcg gcgtcggcgg caagcacgca gtgatccacc cggagattcc
     1621 ggtgatcgac aacatctcgt atctgatcgg cgacggcgac cacccggccc ggctgatgca
     1681 tcccggtgac gcgctgttca cgcccggtga gccggtggac gtgctcgcga cgcctgccgc
     1741 ggcgccgtgg atgaaactct ccgaggccgt tgactatcta cgagccgtgg caccggcccg
     1801 cgccgtccct atccaccagg cgatcatcaa tcccaacgcg cgcggcatct actacggcag
     1861 gctctccgag atgaccgaca ccgacttccg ggtgctcgac cccgaaaacg ccaccacttt
     1921 ctgacggcga gccgaactaa ccggtgtccc tcgccgcggc gcggcccgcg gcccggcccg
     1981 agaagatgca gccgccgagg aacgtgccct ccaacgagcg gtagccgtgc acgcccccgc
     2041 caccgaaacc cgcggcctca cccgcggcat atagtcccgt gaagacgccg ccgtcgtggc
     2101 gcagcacccg cgagtcgaga tcggtttcca gcccgcccaa cgactttcgc gtcaggatgt
     2161 gcagcttcac cgcgatcatc ggcccggcct tcgggtcggt cagccggtgc ggggcgacga
     2221 cacgggtgaa gcggtcaccg aggtagccgc gggccgccct gatcgccgtc acctgggtgt
     2281 ccttggtgaa ccggttggcg acctcccggt cgcgcgcggt cacctccgct tcgacggtgg
     2341 cgtagtccag cgggatgacg ccgggaacgg tgttcatcgc agagaccaac tcacccaacg
     2401 tcttcgcgct gacgaagtcg acacccttgt cgacgaacgc ctgcaccggc gcaggggcgc
     2461 ccggacgcac cctggcgagc acatcgcgga cgctgcgccc ggtgaggtcg ggattctgct
     2521 cctgcccgga cagcgcgaac tccttggcga tgatgcgggc gttcagcacg aaccaggtgt
     2581 agtcccatcc ggtgcgggcg atgtgttcca gcgtgccaag ggtgtcgaaa cccggataca
     2641 gcggtgcagg caggcgtttc ccggaggcat cgagccacag cgacgacggc gcgggcagga
     2701 tccggatgcc gtgcagtggc cagatcgggt cgtaattcgt gatgccctcg gtgtagtgcc
     2761 acatccggtc gctgttgatg acgtgcgcgc ccgccgactc ggtgatgccg atcatccgac
     2821 cgtcgacgtg tgcgggcacg ccgctgagca gttgctcggg cacccggccc atccgcttcg
     2881 gccagttctt gcgaaccagg tcatggttgc cgccgatgcc gccactggcg acgatcaccg
     2941 cctgcgcacg aaactcgaag tcgccgaagg tgtttcgtga cgacgccacc ccgcgcgcgg
     3001 catccgacgg ctccagcacc gcgccgcgca cgccgacgac cgaaccgtct tcgacgatca
     3061 gctcgtcgac gcggtgccgg tgcgcgtagg tcaccctgtc attgccggcg agtcgacggg
     3121 cgaacacgtc gacgatcgcg ggcccggtgc cccacgtgat gtggaagcgg ggcaccgagt
     3181 tgccgtgccc gcgtgcgtca tagccgcccc gctcggccca gccgaccagc ggaaaggtcg
     3241 tgaggccgcg ctcccgtagg aagctgcgct tctcgcctgc cgcgaaatcg acgtaagcgt
     3301 gcgcccactg ccg
//
LOCUS       DASYLX010001069         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1613571,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001069 DASYLX010000000
VERSION     DASYLX010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613571"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGP27_25035"
     CDS             <1..298
                     /locus_tag="VGP27_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854601.1"
                     /translation="HEVFGPVLVVIRYEDDDDAVRIANNSIYGLSGAVFGSEDRALAV
                     ARRIRTGTLSINGGMFFAPDSPFGGYKQSGIGREMGAAGLEEFLERKTIATVVS"
     gene            360..1571
                     /locus_tag="VGP27_25040"
     CDS             360..1571
                     /locus_tag="VGP27_25040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854602.1"
                     /translation="MSSRPLEGIRVLEVAMYGFVPSAGAVLTEWGADVIKVEHAITGD
                     PQRGLRQTGLLRVEGDPNPNIEHANRNKRSIGLDMSVPEGREVLYELARRSDVFLTSF
                     LPGHRTKFGIDVDHIRAVNPKIIYARGSALGPRGEESVKGGYDMTAFWCRAGTAATIT
                     PPGMPGMIGPPGPAYGDTISGTNLAGGIAAALLKRERTGEPSIVDVSLLGSGFWSMGH
                     TVALTKHLGQRMEAFPPGVHGSPINPLVGLYATSDDRYISFVMMQPTKFWADVCKHME
                     LDDLADDPRFATVESIAENTAAATEILTEAMSKRPLTEWSERFATLLGPWAPVQDTLQ
                     AVEDSQIRANEYLVQAGELELVANPVQFDVSPPESGPAPAFAEQTDEILLELGLDWDR
                     IIELKTAGAVT"
     gene            1710..2048
                     /locus_tag="VGP27_25045"
                     /pseudo
     CDS             1710..2048
                     /locus_tag="VGP27_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
     gene            2035..2844
                     /locus_tag="VGP27_25050"
     CDS             2035..2844
                     /locus_tag="VGP27_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854603.1"
                     /translation="MARGSGPERWSTGISLGRGSKPMEAVGGLFAMSKDAVRNVFRRP
                     FQWREFLEQCWFVVKVSLAPTVLVAIPFTVLVSFTLNILLRELGAADLSGAGAAFGAV
                     TQVGPLVTVLIVAGAGATAMCADLGSRTIREEIDAMEVLGIDPVQRLVTPRMLASGVV
                     ALLLNSLVVIIGILGGYTFSIFIQDVNPGAFAAGITLLTGVPEVIISCVKALLFGLIA
                     GLVACYRGLNITGGGAKAVGNAVNETVVYAFMALFAINVVVTAIGIQMSQD"
     gene            2848..3705
                     /locus_tag="VGP27_25055"
     CDS             2848..3705
                     /locus_tag="VGP27_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854604.1"
                     /translation="MGTQTVLRSRFPRLARGLQWPTDQLGRLGDHMLFYFRALGGVPH
                     ATVHFRREIIRLIAEISMGAGTLAMIGGTVVIVGFLTLAAGGTLAVQGYSSLGNIGIE
                     ALTGFLAAFINVRISAPVVAGIGLAATFGAGVTAQLGAMRINEEIDALAAMAIRPIEY
                     LVSTRIVAGMIAITPLYSIAVVLSFLASKFTTVVLFGQSGGLYDHYLNTFLNPVDLLW
                     SFLQAILMAVTILLVHTYFGYFASGGPSGVGVAVGNAVRTSLIVVVSVTLLVSLSVYG
                     SNGNFNLSG"
     gene            3717..4916
                     /locus_tag="VGP27_25060"
     CDS             3717..4916
                     /locus_tag="VGP27_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854605.1"
                     /translation="MKSSAVRPLTGLAMIVAVCLIIALAIGLFRGSFTKTEPVTVISD
                     RAGLVMNSDAKVKMRGVVVGKVNSIESRPDGKAALQLAMDPSQLKLIPSNVKVDIASS
                     TVFGAKSVDMVSPDNPSPQPLRPGQVIQSEHVVVEINTVFQQLVQVLDKIDPAKLNQT
                     LGAIATAFNGRGEKFGKTLTDFNAFLAKIEPSLPNLSHDLETAAPTLGAYADAAPDLI
                     RTIGSTTQISNTIVDQQQELDQFLVSSIGLADIGNDVIGGNEPALADVLHLLVPTTDL
                     LNRYNESLYCSIAGLAVMANSPPLPGNNSAVVVSAGLTLGTERYRYPSDLPKVAGKGR
                     PYCKELGLPNVPPEFRVPAIVTDVGANPYQYGNQGILLNSAGLKDWLFGPIAGPPRNS
                     AQIGMPG"
     gene            4913..>5773
                     /locus_tag="VGP27_25065"
     CDS             4913..>5773
                     /locus_tag="VGP27_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854606.1"
                     /translation="MTRATGTLIKFTAFALVMTILTAFLFLIFSQTRTGSTNGYSAVF
                     TDASRLKTGDTVRVAGIRVGTVKGVSLQSDRKVLVEFDTDRSLVLTTGTKAQIRYLNL
                     TGDRFLELADSPGSTKILSAGSRIPVDRTAPALDLDLLLGGLKPVIQGLNPQDVNALT
                     SSLIQILQGQGGTLESLFSKTSSFSNSLADNSQVIEQMIDDLRTLLDTLSKDGDQFSG
                     SIDRLERLVSGLSTDRDPIGEAITSLDNGTASLADLLGRARPPLAGTVDQLNRLAPLV
                     DGAHKDYLDAT"
BASE COUNT         1031 a         1813 c         1855 g         1074 t
ORIGIN      
        1 gcacgaggtg ttcgggccgg tgctcgtcgt catccgctac gaagacgacg acgacgccgt
       61 ccggatcgcc aacaactcca tctacggcct gtccggcgcc gtgttcggca gcgaggaccg
      121 cgccctcgcg gtggcgcgcc gcatccgcac cggcaccctc tcgatcaacg gcggcatgtt
      181 cttcgctccc gacagtccgt tcggcggcta taaacagtcc ggcatcggca gggagatggg
      241 cgccgcgggg ctcgaagagt tcctggagcg caagacgatc gcgacggtcg tctcatgagc
      301 ccccgaagac gagcgcgagc gaggaacggg cgaggaccgg agcgagtggg agtcgaggca
      361 tgagttctcg gccgctggag ggcatccgcg ttctcgaggt cgccatgtac ggcttcgtcc
      421 cgtcggcggg tgcggtgctc acggaatggg gcgccgacgt catcaaggtc gagcacgcaa
      481 tcaccggtga cccgcagcgc gggctgcgcc agaccggact gctgcgcgtc gagggcgatc
      541 ccaacccgaa catcgagcac gccaaccgca acaagcgcag catcgggctc gacatgtcgg
      601 tgcccgaagg cagggaagtg ctctacgagc tggcccggcg cagtgacgtc ttcctgacca
      661 gcttcctgcc cggacaccgc acgaagttcg gcatcgacgt cgaccacatc cgagccgtta
      721 atccaaagat catctatgca cggggcagcg cactgggtcc tcgcggcgag gagtcggtca
      781 agggcggcta cgacatgacc gccttctggt gccgtgcggg taccgcggcc accatcaccc
      841 cgccgggcat gccggggatg atcgggccgc ccggccccgc ctacggcgac accatcagcg
      901 gcacgaacct cgcaggaggc atcgcagccg cgctgctgaa gcgggagcgc accggagagc
      961 cgtccatcgt cgacgtttcg ctgctcggca gcgggttctg gtcgatgggg cacaccgtgg
     1021 cgctgaccaa gcacctcggt cagcggatgg aggcctttcc gccaggtgta cacgggtcgc
     1081 cgatcaaccc gctggtgggc ctgtacgcga cgtccgacga ccgctatatt tccttcgtca
     1141 tgatgcagcc caccaagttc tgggccgacg tgtgcaagca catggaactc gacgacctcg
     1201 ccgacgatcc gcggttcgcg accgtcgagt cgatcgccga gaacaccgca gcggcaacgg
     1261 agatcctcac cgaggcgatg tccaaacgcc cgctgactga atggagcgag cggttcgcga
     1321 cgctgctcgg accgtgggca ccggtgcagg acaccctcca ggccgtcgag gacagccaga
     1381 tccgggccaa cgagtacctc gtacaagcgg gcgaactcga gttggtggcg aatccggtgc
     1441 agtttgacgt cagtcctccc gaatccggac ccgcgccggc ttttgcagag cagactgacg
     1501 aaatcttgct tgaactgggg ttggattggg accgcataat cgagctcaag acggccggcg
     1561 ccgtcaccta gatcgcagag gatcacatgc cctaccttgc tctatcggca ctgacctgcg
     1621 gtgttgcgga ccatctcatc accaggtggc cggcgacgtc gaggacgcca gcattcgtgc
     1681 tgtcgaagca caccgcgtcg cacgccaccg ccgacgtcga catcgctgaa attgacgttt
     1741 ccttcacggg aattgatgaa gtcggttatg aaggtgttgg ctttgccgag cggttggggg
     1801 gctacgagct tgtcgaggct gaggtgacaa gtgtcggggg tgcgctgccg gtgaacccca
     1861 gcggaggcaa gggccatcct ccgggggcga cgagcgtcga acagggcgtg gagctattcg
     1921 cgcaactccg aggggaggcg gtcaaccagg tggacggcgc gcgggtcgca ttgacccgag
     1981 acatcggccg tcggacggca gtttcgacgg ccatcatcct ggaaggaccg gccaatggcg
     2041 cgcggtagtg gacccgaacg gtggtcgaca ggcatctcgc tcggccgcgg ttccaagccg
     2101 atggaggccg tcggcggatt gtttgcgatg tcgaaggacg cggtgcggaa cgtcttccgg
     2161 cggccgtttc aatggcgtga gtttctggag cagtgctggt tcgtcgtgaa ggtatcgctg
     2221 gcgccaacgg tgttggtggc gatcccgttc accgtgctgg tgtcgttcac gctcaacatc
     2281 ctgcttcgcg aattgggcgc cgcggacttg agtggtgccg gtgcggcgtt cggcgcggtg
     2341 acccaggtcg gtccgctggt gacggtcttg atcgtggcgg gcgcgggcgc cacggctatg
     2401 tgtgcggatc ttggttcgcg cacgattcgc gaagagatcg acgccatgga agtgctgggc
     2461 atagatccgg tgcaacgcct ggtgacccct cggatgctgg cctcgggtgt ggtggcccta
     2521 ctgctcaaca gcctggtggt gatcatcggg atattgggcg gctacacgtt ttcgatcttc
     2581 atccaggatg tcaatcctgg tgcgttcgcg gcgggcatca ccctgttgac cggtgtccca
     2641 gaggtgatca tctcgtgtgt gaaggcgttg ctgttcgggt tgatcgccgg gttggtggcc
     2701 tgctaccgcg ggctcaacat cactggtggt ggggcgaagg cggtgggcaa cgccgtcaac
     2761 gagaccgtgg tgtacgcatt catggcgctg tttgcgatca acgtggtcgt gacggccatc
     2821 ggcatccaga tgagccagga ctaaccgatg ggaacacaaa cggtactgcg cagtcgcttt
     2881 ccgcggctgg cccggggact ccagtggccc accgaccagt tgggccgcct cggcgaccac
     2941 atgttgttct actttcgcgc cctgggcggg gtgccgcatg cgaccgttca tttccgtcgc
     3001 gagatcatcc ggctgatcgc cgaaatctcc atgggggcag ggaccttggc gatgatcggc
     3061 ggcaccgtgg tcatcgtcgg cttcctcact ctggccgccg gtggaacact cgcggttcag
     3121 gggtacagct ccctcggcaa catcggcatc gaagcgctga ccgggttctt ggcggccttc
     3181 ataaacgtgc gcatctctgc cccggtggtg gcgggcatcg gcttggcggc caccttcggc
     3241 gcgggtgtga cggcccagct gggcgccatg cgcatcaacg aggagatcga cgcgctcgcg
     3301 gccatggcga tccgccccat cgaatacctg gtctccactc ggatcgtcgc ggggatgatc
     3361 gcgatcaccc cgctgtactc gatcgcggtg gtcctgtcgt ttctggcttc caagttcacg
     3421 acggtggtgt tgttcgggca gtcgggcgga ctgtatgacc actatctcaa cactttcctc
     3481 aacccggtcg acttactgtg gtcgttcctg caggcgatac tgatggcggt cacgatcttg
     3541 ttggtacaca cgtatttcgg gtatttcgcc tccggcgggc cctccggtgt cggcgtcgcc
     3601 gtgggtaacg cggtgcgcac ctccctgatc gtggtcgtct cggtcaccct tctggtgtcg
     3661 ctgtccgtct acggttccaa cggcaacttc aacctgtcgg gctaggtggc acgccgatga
     3721 aaagtagtgc ggtacgcccg ctgacgggct tggcgatgat cgtcgccgtc tgcttgatca
     3781 tcgccttggc aatcggcttg tttcggggca gcttcaccaa aaccgaacca gtgacggtca
     3841 tttcggatcg cgccggtctg gtgatgaaca gcgacgctaa ggtcaagatg cgtggcgtcg
     3901 tggtcggcaa agtcaattcg atcgagagtc ggcccgacgg caaggcggcg ttgcagctgg
     3961 cgatggatcc gtcgcagctg aaactcatcc cgtccaacgt gaaggtcgac atcgcatcgt
     4021 caacagtgtt cggcgccaag tctgtcgaca tggtctcccc cgataaccca tcgccgcagc
     4081 cactccgccc gggtcaggtg attcaaagcg agcacgtcgt ggtcgagatc aacaccgtct
     4141 tccagcagtt ggtgcaggtg ctggacaaga tcgatcccgc caagctcaat cagacactcg
     4201 gagccatcgc aacggcgttc aacggccggg gcgagaagtt cggcaagacg ctgaccgact
     4261 tcaacgcgtt cctggccaag atcgaaccga gcctgcccaa tctcagccac gacctcgaaa
     4321 ccgctgctcc gacactgggt gcgtacgcgg acgcggcgcc cgacctcatc aggaccatcg
     4381 gcagcaccac acagatcagc aataccatcg tcgaccagca acaggagctt gaccagttct
     4441 tggtcagctc cattggtctg gccgacatcg gaaacgacgt gatcggcggc aacgaaccgg
     4501 cgctggccga tgtgctgcac ctgctcgtac ccaccacgga tctgctcaac cggtacaacg
     4561 aatcgctgta ttgctcaatt gccggacttg ccgtgatggc gaattcgccg ccgcttccag
     4621 gtaacaacag cgcggtggtg gtgtctgccg gcctgacgct cggtaccgag cgctatcgct
     4681 atccgagtga cctgccgaag gtagccggca agggccggcc atactgcaag gaactgggtc
     4741 tgcccaacgt tccccccgag tttcgagtgc ctgccatcgt caccgacgtg ggcgcgaacc
     4801 cgtatcagta cgggaatcag ggcatcctgc tgaactccgc cggtctcaag gattggttgt
     4861 tcgggccaat tgccggtccg ccgcgcaact ccgcacagat tggaatgcct gggtgacacg
     4921 cgcgacgggc acgctcatca agttcacggc tttcgcgctc gtgatgacga tattgacagc
     4981 cttcctgttc ttgatcttca gccagacgag aaccggttcg acgaatggct actctgcggt
     5041 cttcaccgac gcgtcgcgac tcaaaacggg cgacaccgtg cgtgtcgcgg gaatccgagt
     5101 cggcactgtg aagggggtgt cgctgcaatc ggaccgcaag gtgctcgtcg agttcgatac
     5161 tgatcgcagc ctcgtgctga ccaccggaac caaagcgcag atccgctacc tcaacctgac
     5221 cggcgatcgc ttcctcgaac tcgccgacag ccccggatcg acgaagatcc tgtcggccgg
     5281 ctcgcggata cctgtggacc ggacggcacc cgccctggac ctcgacctgc tgctcggcgg
     5341 actgaaaccc gttatccagg gcctcaatcc gcaagacgtg aacgcgctca cctcgtcgct
     5401 gattcaaatt ctgcagggcc aagggggaac cctcgaatcg ctgttctcaa agacatcctc
     5461 gttctcgaac tcattggcgg acaacagcca ggtcattgag cagatgatcg acgacctgcg
     5521 aacactgctg gacacgttgt ccaaggacgg cgaccaattc tctgggtcga tcgaccgact
     5581 cgagcgactg gtcagtggct tgtcgaccga ccgcgacccc atcggtgagg cgatcacgtc
     5641 tctagacaac ggcaccgcct cgctggccga cttgctcggc cgggcacggc cgccgctggc
     5701 cggcacagtc gaccagttga accggctggc tcctctggtg gacggcgcgc acaaggatta
     5761 tctcgacgcc acc
//
LOCUS       DASYLX010001070         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1940083,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001070 DASYLX010000000
VERSION     DASYLX010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940083"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VGP27_25070"
     CDS             complement(<1..1163)
                     /locus_tag="VGP27_25070"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEV7854607.1"
                     /translation="MPEPTILLLSTSDTDLIAARSSGARYRWANPSRLVDGELTEQID
                     GADLVVVRILGGYRSWQDGIDTVIGSGVPTVVVSGEQAPDAELMSHSTVPAGAAVQAH
                     IYLAQGGMENLRQLHAFLCDTALMTGFGFAPPAIIPNWGVLERTSSNGGGPTIAVLFY
                     RAQQLAGNTGYVQALCGAIEDAGGRALPVYCASLRTAESDMLDLLKSADAMVTTVLAA
                     GGAVPATVSAGGNDDTWNVAHLAALDIPILQGLCLTSSRSQWHDNDDGLSPLDVATQV
                     AVPEFDGRIITVPFSFKEIDDEGLISYVADPERCARVAGLAVRHARLRAVAPKDKRVA
                     VVFSAYPTKHARIGNAVGLDTPASAVALLRAMRVAGYDIGDIPGVDAQDGDAL"
     gene            1516..>1661
                     /locus_tag="VGP27_25075"
     CDS             1516..>1661
                     /locus_tag="VGP27_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase"
                     /protein_id="HEV7854608.1"
                     /translation="MPSSVRVVKLGAHIGARVDGIRLADGVDATAAAHINAALLEHKV
                     IFFR"
BASE COUNT          279 a          558 c          544 g          280 t
ORIGIN      
        1 atcagcgcgt cgccgtcctg tgcgtcgaca cccgggatgt cgccgatgtc gtagccggcc
       61 acccgcatgg cccgcaacag ggcaaccgcg ctggccgggg tgtcgagccc gaccgcgtta
      121 ccgatgcggg catgtttggt cggatacgca gagaacacga ccgccacgcg cttgtccttc
      181 ggcgcgacgg cccgcagtcg tgcgtgccgc acagcgaggc ccgccacgcg ggcgcagcgt
      241 tcgggatcgg cgacgtagga gatcaggccc tcatcgtcga tttccttgaa cgagaacggg
      301 accgtgatga tccgtccgtc gaactccggc accgccacct gcgtcgcgac gtcgagcggg
      361 ctgagaccgt cgtcgttgtc gtgccattgt gaccgcgagc tggtcaagca caggccctgc
      421 aggatcggga tatccagtgc ggcaaggtgt gccacgttcc aggtgtcgtc gttgccgcct
      481 gccgacacgg tggccggaac cgctccgcct gcggccagca cggtggtcac catggcgtcg
      541 gccgatttca gcaagtccag catgtcgctt tcggcggtac gcaacgaggc gcagtagacg
      601 gggagcgcgc gcccgccggc gtcttcgatc gcaccgcaaa gcgcttggac gtagccggtg
      661 ttgcccgcca gctgctgagc ccggtagaag agcacggcta tcgtcgggcc accaccgttg
      721 ctcgacgtgc gttcgagcac accccagttc gggatgatcg cgggcggcgc gaacccgaag
      781 ccggtcatca gcgcggtgtc gcacagaaat gcgtgcagct ggcggagatt ctccatgccg
      841 ccctgagcga gatagatgtg cgcctgcacc gccgccccgg ccggaaccgt ggagtggctc
      901 atcagctcgg cgtccggagc ctgctcaccg ctgaccacca ccgtcggcac gccgctgccg
      961 atcacggtgt cgatgccgtc ctgccaggat cggtagccac cgagtatccg caccacgacc
     1021 aggtccgcgc cgtcgatctg ctcggtcagc tcaccgtcca cgagccgcga cgggttggcc
     1081 caccgatacc gcgccccact cgaacgggcg gcgatcaggt ccgtgtcaga cgtcgacagc
     1141 aacaggatgg tgggctcagg cacccaccat tcctatcgca gtgggcggtc agtcgccgaa
     1201 gcggccgtgc caggtctgtg ggtagggcag gccggtcacg gcggcaagga cgaggagtac
     1261 agccccgaag acgatcaagg agaacatcgt tggtccttcc gtacgttgtt gcacacacgt
     1321 gcaactgcga gcaccgtacg acgacaccgg cgattaatga tcgacatgtg cctgacggaa
     1381 ctcgcagacc agcggtgtga cgacggtcac atgctcgtct cgcccacttg cgttctcgct
     1441 gagttgaaat cttggcggcg agccgacggg ccgccatcct gctacggggt gttctgcgtc
     1501 tgtggaggtg aactcgtgcc ctcatccgta cgtgtcgtca agctcggggc tcatatcggc
     1561 gcccgcgtcg acggcattcg ccttgccgat ggtgtcgacg ccaccgccgc cgctcatatc
     1621 aatgcggcgc tgctcgagca caaggtgatc ttcttccgcg a
//
LOCUS       DASYLX010001071         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1940112,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001071 DASYLX010000000
VERSION     DASYLX010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940112"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGP27_25080"
     CDS             <1..247
                     /locus_tag="VGP27_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854609.1"
                     /translation="SIADGRFDPVVAARAKLQIGRSGRHIAQEAIQMHGGIGMTAEYP
                     VGHYAARLTAIDNTFGTADDQLRALIKDIGTYGTVSL"
     gene            complement(251..1168)
                     /locus_tag="VGP27_25085"
     CDS             complement(251..1168)
                     /locus_tag="VGP27_25085"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV7854610.1"
                     /translation="MSVRELIVLGTSSQVPTRYRNHNGYLLRWDGEGLLFDPGEGTQR
                     QMLFAGVPASRVTRLCITHFHGDHCLGVPGVVQRLSLDGVAHPVHAHFPASGQDFFAR
                     LRHASIYYDRVDVREEPVAEDGPVATGAFGVLEARRLDHPVDAVGYRLVEPDGRRFVP
                     ELLSRFGISGPAVGELQQAGSVDIGGRVTATADVSAPRPGQRFAFVMDTRLCDAVYAL
                     ADGVDLLVIEATFLNEDAELATRYGHLTARQAARVAQECGVRRLVLTHFSQRYTDAGQ
                     RYRDEAGEVYTGDLVVAEDLMRIPVPKRV"
     gene            complement(1185..2072)
                     /locus_tag="VGP27_25090"
     CDS             complement(1185..2072)
                     /locus_tag="VGP27_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEV7854611.1"
                     /translation="MTAAKRVPCSDQSLARDDPMYGTASAGYSWMLLELPGAWGHSAF
                     LQSPAVIEPALGRAVVRRIESAKMRVAAIRKPGRRSSTPRWRWVVAHSRVGGEALYTG
                     EVSDPREYLDLALDGSDGTQSADPVVAICAHGKHDQCCAVRGRSACNAIAAEYPEFTW
                     ECSHLGGDRFAATMLVLPEGLCYGRVDTADPPHLIRLYLEGRLDNRFLRGRTSLPHAV
                     QAAQYFAREAYGDDRIDALHPQSVDHLDGEIRVVLDGESGAITVVLKELLTEPLLSQC
                     DARVPGRVRTFTLVSITSA"
     gene            complement(2069..>2543)
                     /locus_tag="VGP27_25095"
     CDS             complement(2069..>2543)
                     /locus_tag="VGP27_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEV7854612.1"
                     /translation="TVDSFRTSLPMGGDPADHDESIATVTKVMAEMVDSLRDDASVLS
                     GGAAATLIERHADRTRPVAVRPLASLRAADRAGTTEVQWRHGLLATVEHTAGRVVLRL
                     PDRVMTFPVSCAEAVAALHRGLVADAGGLPGLDRADGTVLIRRLLREAVVVPFKG"
BASE COUNT          450 a          893 c          821 g          379 t
ORIGIN      
        1 gtcgatcgcc gacggcaggt tcgacccggt cgtcgcggca cgcgccaagc tgcagatcgg
       61 gcggtccgga cggcacatcg cccaggaggc gatccaaatg cacggcggca tcggaatgac
      121 tgccgagtat ccggtcggcc actacgccgc gcgactcacg gccatcgaca acacgtttgg
      181 aacggccgac gaccaactgc gggcactcat caaagacatc ggcacgtacg gaaccgtgtc
      241 gctctagcgg ctatacccgc ttgggcaccg gtatgcgcat caggtcttcg gcgacgacca
      301 gatcaccggt gtacacctcg cccgcctcgt cgcggtagcg ctggccggcg tcggtgtagc
      361 gctgcgagaa gtgcgtgagc acaagtcggc gcaccccgca ttcctgcgcc acccgcgctg
      421 cctggcgtgc ggtcagatgg ccgtagcggg ttgccaattc ggcgtcctcg ttcagaaagg
      481 tcgcctcgat caccaacagg tcgactccgt cggccaaggc gtagacggcg tcgcacaacc
      541 gggtgtccat gacaaaggcg aatcgctggc cggggcgcgg cgcgctgacg tccgccgtgg
      601 cggtgacccg gccaccgatg tcgaccgacc ccgcctgctg caactcgccc acagcggggc
      661 cgctgatacc gaaccggctc aacaactccg gcacgaagcg ccggccgtcc ggttcgacga
      721 ggcggtaccc gaccgcgtcg acagggtgat cgagccgccg cgcctccagg acgccgaagg
      781 caccggtggc gaccggtccg tcttcagcta ccggttcctc gcgcacatcg accctgtcgt
      841 agtagatgct ggcgtgccgc aggcgggcga agaagtcctg tcccgacgcg gggaagtgcg
      901 catgaaccgg atgcgccacg ccgtccagtg acaaacgttg caccactccg ggcacaccta
      961 ggcagtgatc accgtggaag tgggtgatgc acaaccgcgt caccctgctc gcaggcacac
     1021 cggcaaacag catctgccgc tgcgtgccct cgcccgggtc gaacagcaag ccctcgccgt
     1081 cccagcgcag cagatagccg ttgtgattgc ggtatcgggt cggcacctga ctggaggtgc
     1141 cgagcacaat cagctcacgc actgacaccc gtcgacacta cgactcaggc cgacgtgatc
     1201 gagacgagcg tgaaggtccg aacccggccg ggcacccgtg catcacactg cgacagcagc
     1261 ggctccgtca ggagttcttt gaggaccacg gtgatcgcac ccgattcgcc gtcgagcacc
     1321 acgcggatct cgccgtcgag gtggtcgaca ctttgcggat gcaacgcgtc gatgcggtcg
     1381 tcaccgtagg cctcgcgggc gaagtactgg gcggcttgaa ccgcatgcgg tagcgacgtg
     1441 cgcccgcgaa ggaacctgtt gtccagccgc ccctcgagat acaggcgaat cagatgaggt
     1501 ggatcggcgg tgtcgacgcg cccgtagcac agcccttccg gcagcaccag cattgtcgcc
     1561 gcgaaccggt caccgcccag atgcgagcac tcccaggtga attcggggta ctccgcggcg
     1621 attgcgttac aggcgctgcg ccctcgaacc gcgcagcact gatcatgctt gccgtgggca
     1681 cagatcgcca cgacgggatc ggccgactgc gtcccgtcgg atccgtccag agcgagatcg
     1741 agatactcgc gggggtcgct cacttctccg gtgtacagcg cttcgccgcc caccctggaa
     1801 tgcgcgacga cccagcgcca ccgcggtgtg gacgacctgc gaccgggctt acgtatcgcc
     1861 gcgaccctca tcttcgccga ttcgatccgc cggacgacgg cccgtccgag agccggctcg
     1921 atgacagcag gcgactggag gaacgccgag tgtccccatg cgccgggcaa ctcgagcaac
     1981 atccaggagt acccggcgga ggcggtgccg tacatggggt cgtcgcgggc cagcgactgg
     2041 tcgctgcacg gcactcgttt ggcagcggtc atcccttgaa cggtacgacg acggcctctc
     2101 gcagcagcct gcggatcaac accgtgccgt cggcacggtc cagaccgggt aagccgcccg
     2161 catcggcgac gaggccgcgg tgcagcgccg cgaccgcctc tgcacacgac accgggaacg
     2221 tcatcaccct gtccgggagt cgcagcacga cccggcccgc cgtgtgttcg acggtcgcga
     2281 gcagcccatg acgccattga acttctgtcg tgcccgctcg atctgccgcc cgaagcgacg
     2341 caagcggacg caccgcgacg ggccgtgtcc gatccgcgtg acgctcgatc aatgttgcgg
     2401 cggcgccacc gctgagcaca gaggcgtcgt cgcgcagcga gtccaccatc tcggccatca
     2461 ccttcgtcac cgtcgcgatg ctctcatcgt gatcggccgg gtcgccgccc atcggcaggg
     2521 acgtcctgaa ggagtcgacg gtc
//
LOCUS       DASYLX010001072         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1172888,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001072 DASYLX010000000
VERSION     DASYLX010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172888"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VGP27_25100"
     CDS             <1..1018
                     /locus_tag="VGP27_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854613.1"
                     /translation="KLGSAAERLDMAGRAVVLADGTRVAYDVLVVATGAAARPSPWSV
                     DSGVHVVRTLDDTHELARALAATGPVVVVGGGFIGAEVAATAHAAGRDVTIVDPLAAP
                     IGRIVGDDLGAMLAGVHARHGVRTRFGIGVESVEGRAGTLRVTLTDGEMLSAATVVVG
                     IGAIPNDGWLASSGLLIDDGVVCDEFCRTVDHPDVYAVGDVARWRHPGHQEDVRVEHW
                     TNAAEQSACVAHNICHPGTPRAYAPTEYVWSDQYDWKIQIAGRPNRSALQRIIGDIDG
                     DPAQVAAVYGDRDGLLRGAVTVNWPKALMLCRRMIGAGTAVADCLAELEGLPRLNAMK
                     TAGR"
     gene            1026..1811
                     /locus_tag="VGP27_25105"
     CDS             1026..1811
                     /locus_tag="VGP27_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7854614.1"
                     /translation="MTTAAVPITTRAYGPTPQYPLESVDNALQILMLLVTRTELRLTD
                     VSVHLGVASSTAHRLLAMLQYRGLIRQDSRTKAYQTGPSLDLLAFTVLKQLDVRDRAR
                     PVLAKLNADLQETVHLGRLEGAEVDFVASIESPQALRVANRQGVAMPAHCTSTGKVLL
                     AALTPEELDILYPDPDLIQMTRHSIASTAALKSELAAVRRRGHATSNEEGEEGVVSIA
                     VPIRNGTFALNVSVPVSRMTRDLRRTALGQLAVAAAEIEALLP"
     gene            1808..>2697
                     /locus_tag="VGP27_25110"
     CDS             1808..>2697
                     /locus_tag="VGP27_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEV7854615.1"
                     /translation="MSGLERLELTYGGELYDRTRRLYSGEVVPEGIRLRYLHTAIEDL
                     FWRQGRYGEFDLAEYSMGAYLSTVDDPDRPFTALPIFPSRVFRHSSVYVNADTDVSDV
                     AALNGAVIGTPEWSMTASLWMRGILGEHHGVDLASIRWRTGGLEEPGRQEKAPVTPPA
                     HFDVSHIGDADTLSAQLIRGDLDGLITARAPRAFLQGDRRIRRWWPDFRSAEQAYFEK
                     TQIVPIMHVVVIRRALLAAHPWVANNLVDAFERARTPVQRELLDTAVCTSSLIWESSY
                     AEAEQAMLGDPFRCGVAENAA"
BASE COUNT          450 a          888 c          913 g          446 t
ORIGIN      
        1 gaagctcggc agcgccgccg agcgcctgga catggccggc cgcgcggtcg tgctcgccga
       61 cggcacccga gtggcgtatg acgtccttgt cgtcgctacc ggtgcggccg ctcggccgtc
      121 cccatggtcg gtggactcgg gtgttcacgt ggtgcggacc ctcgacgaca cgcatgaact
      181 ggcccgcgcc cttgcggcga ccggaccggt agtcgtcgta ggcggtggat tcatcggtgc
      241 cgaggtggct gccacggcac acgccgccgg gcgggacgtc acgatcgtcg acccgctcgc
      301 ggcgccgata ggccggatcg tcggagacga tctcggggcg atgctcgccg gcgtgcacgc
      361 ccggcatgga gtgcggacga ggttcggcat cggtgtggag tcggtcgagg gacgcgcggg
      421 tactctgcgc gtcaccctca ccgacggtga gatgctttct gccgcaacgg tggtcgtcgg
      481 catcggcgcg attcccaacg acggatggct ggcatcgtcg ggccttctca tcgacgacgg
      541 cgtggtctgc gacgaattct gtcgcaccgt cgaccacccg gacgtctacg ccgttgggga
      601 cgtggcccgc tggcgccatc ccggacatca ggaagacgtc cgcgtcgagc actggacgaa
      661 tgccgccgag cagtccgcct gtgtggcaca caacatctgc catcccggca cgccgcgcgc
      721 atacgcgccg actgagtacg tatggagcga ccagtacgac tggaagatcc agatcgccgg
      781 gcggcccaac cggtctgcgc tgcaacggat catcggtgac atcgacggcg acccggcaca
      841 ggttgcggcg gtatacggcg accgggacgg cctgctgcgc ggggcagtca cggtcaactg
      901 gccgaaggcg ctgatgttgt gtcgccggat gatcggcgcc ggcaccgccg tcgccgactg
      961 cctcgcggaa ctcgagggtc taccgcggct caatgccatg aaaacggccg gaaggtaggc
     1021 acgtcgtgac gacggcagca gtaccgataa cgacaagggc ctacgggccc actccccaat
     1081 atccgctgga atcggtcgac aacgccctgc agatcctgat gctcctggtg acgcgaacgg
     1141 agcttcggct caccgacgtg agcgtccatc tcggcgttgc cagttcgacg gcacaccggc
     1201 tgctggccat gctgcagtac cgcgggctga tccggcagga ttcgagaacc aaggcctacc
     1261 agacaggtcc aagtctggat ctactggcat tcacggtgct gaagcagctc gatgtccgcg
     1321 accgtgcgcg cccggtgctg gcgaagctga acgccgatct gcaggagacc gtacacctgg
     1381 ggcggctcga aggagccgag gtcgacttcg tggcgtccat cgagagtcca caggcgctgc
     1441 gggtcgcgaa ccgccaaggc gtcgccatgc ccgcgcactg cacgtcgacc ggaaaggtgc
     1501 tgctcgcagc cctaaccccg gaggagctgg acattctgta ccccgatccc gatttgattc
     1561 agatgacgcg gcattcgatc gcgtcgacgg ccgctctcaa gtccgaactc gccgcggtgc
     1621 gcaggcgcgg acatgccacc agcaatgaag aaggggagga aggcgtggtg tccatcgccg
     1681 ttcctatacg caacggcacg ttcgccctga acgtttcggt gccggtcagt cggatgacca
     1741 gggacctgcg gagaacggcg ctcggccagc tggccgtcgc cgccgccgag atcgaggcgc
     1801 tgcttccgtg agcggtcttg aacgcctcga attgacttac ggcggtgagc tttacgaccg
     1861 gacccggcgt ctgtacagcg gcgaggtggt tccggagggt atccgtctgc gctacctgca
     1921 cacggccatc gaagatctct tctggcggca gggcaggtac ggcgagttcg acctggccga
     1981 atactcgatg ggcgcttacc tctccacggt ggacgacccg gatcggccct tcaccgcgct
     2041 gccgatcttc ccgtcgcggg tctttcggca ctcgagcgtc tacgtcaacg cggacacaga
     2101 tgtttccgac gtggccgccc tcaacggtgc cgtgatcggc acccccgagt ggagcatgac
     2161 cgcgtcgctg tggatgcgcg gcatcctggg cgaacaccac ggcgtggacc tggcgtcgat
     2221 ccgctggcgc acgggaggtc tcgaggaacc cggccgacag gagaaggcgc ccgtgacgcc
     2281 gccggcgcac ttcgacgtgt cgcacatcgg cgacgcggac acgctgagtg cccagctgat
     2341 ccgtggcgat ctggatggtc tgatcaccgc cagggcccca cgcgccttcc tgcagggcga
     2401 tcggcggatc cgtcggtggt ggcccgactt ccgttcggct gagcaggcat atttcgagaa
     2461 gacgcagatc gttccgatca tgcatgtggt ggtcatcagg cgcgcactgc tcgccgctca
     2521 tccctgggtc gccaacaacc tcgtcgacgc attcgaacgg gcacgcacgc ccgtacagag
     2581 ggagcttctc gataccgccg tctgtaccag cagcctgatc tgggagtcct cgtacgcgga
     2641 ggcggaacag gcgatgctcg gtgatccgtt ccgctgcggt gtcgccgaga acgccgc
//
LOCUS       DASYLX010001073         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3808525,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001073 DASYLX010000000
VERSION     DASYLX010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808525"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VGP27_25115"
     CDS             complement(<1..1245)
                     /locus_tag="VGP27_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV7854616.1"
                     /translation="MSRDKSSVAAMLGARSMALVGASPRPDSFGARMIIEAQRGSARF
                     HLVNPRYDRIGERACVPSIAEVDEPVDVALLGVSDAVLPEQLAAAAAAGARSAVLFGS
                     ANGMRDEITAIAADAGMALCGAGCMGFVNNAIGVRALGYLEPDRMPEGVVSLVTHSGS
                     AFSTLLRARRAFGFRLAVSSGQELVTDTADYVDYALDDPETRIVGLLLETPRAAPRLR
                     NRLLRAAEQDVPVVILTVGGSPRGRAMVAAHSGALAGERAAWDAFCTATGAVHVSDLA
                     EFSDTLELFSAGRRARPGGIATVHDSGAERALAADLAHDLGVEFADLAPDTLATIGSM
                     LDDGLAATNPLDVWGTGADTRSLFGACLRAMVDDPAVAVTALAVDLVTEFDGDTAYAD
                     AVTDVAKHTGAPIAVMASVASAI"
     gene            complement(1242..>1575)
                     /locus_tag="VGP27_25120"
     CDS             complement(1242..>1575)
                     /locus_tag="VGP27_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854617.1"
                     /translation="YADRLIDLYDQVAPGFKSSITHRDIVGPHEMEQEYGLLGGNIFH
                     GELSLEQLFHMRPAPGYADYRTPIAGLYNGSSGTHAGGGVCGIPGWQAARAALADKKR
                     GLKRFRRV"
BASE COUNT          249 a          557 c          547 g          222 t
ORIGIN      
        1 gatcgccgac gccaccgatg ccatcaccgc gatcggcgcg ccggtgtgct tggcgacgtc
       61 ggtgaccgca tcggcgtagg cggtgtcacc gtcgaactcg gtgaccaggt cgacggcgag
      121 cgcggtcacc gcgacggccg gatcgtcgac catcgcgcgc aggcaggcac cgaagaggct
      181 tctggtgtcg gcgccggtgc cccacacgtc cagtgggttg gtggcggcca gcccgtcgtc
      241 gagcatgctg ccgatggtgg ccagcgtgtc cggggcgagg tcggcgaact cgacgccgag
      301 gtcgtgcgca aggtcggcgg ccaatgcccg ctcggcaccc gaatcatgca ccgtggcaat
      361 gcctcccggc ctggcccggc ggccggcgga gaacagttcg agcgtgtcgc tgaactcagc
      421 caggtcgctg acgtgtaccg cgccggtcgc cgtgcagaac gcgtcccatg ccgcccgctc
      481 cccggccagc gcgcccgagt gggcagccac catggccctc ccccgcggtg atccaccgac
      541 ggtgaggatc acgacgggca cgtcctgctc cgcggcgcgc agcagcctat tgcgcagtcg
      601 cggtgcggcg cgcggtgttt cgagaagcag tccgacgatg cgcgtttcgg ggtcgtcgag
      661 cgcgtagtcg acgtaatcgg cggtgtcggt gacgagttcc tggcccgacg acaccgccag
      721 ccggaagccg aaagcgcggc gggcccgcaa caatgtggag aacgccgagc ccgaatgggt
      781 gacaaggctg acgacaccct cgggcatccg gtccggctca aggtagccca gcgcccgaac
      841 gccgatcgcg ttgttcacga agcccatgca gccggcaccg cacagcgcca ttccggcgtc
      901 ggccgcaatg gccgtgatct cgtcacgcat cccgttggcg gacccgaata gcacggcgga
      961 ccgggcaccc gccgccgcgg cggccgccaa ctgctcgggc aacacggcgt cggaaacacc
     1021 gagcagtgcc acgtcgacgg gttcgtccac ctcggcgatc gacggcacgc acgcccgctc
     1081 gccgatgcga tcgtagcgag ggttcaccag atgaaagcgc gccgaacccc gttgtgcctc
     1141 aatgatcatg cgggcgccga agctgtccgg ccgcgggctg gcgccgacca gcgccatcga
     1201 ccgcgccccg agcatcgcgg cgacggagga cttgtctcga ctcacactcg ccggaacctc
     1261 ttcagccctc gcttcttgtc ggcgagcgcg gcgcgggccg cctgccaacc ggggatgccg
     1321 cagacgccac cgcccgcatg cgtgccggag ctgccgttgt agagcccggc gatcggcgtg
     1381 cggtagtcgg cgtaccccgg tgcgggccgc atgtggaaga gctgctccaa cgacagctcg
     1441 ccgtggaaga tgttgccgcc caacaacccg tactcctgct ccatctcatg cggcccgacg
     1501 atgtcgcgat gggtgatcga cgatttgaag ccgggcgcca cctggtcgta gaggtcgatg
     1561 agccggtcgg cgtag
//
LOCUS       DASYLX010001074         7917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1282230,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001074 DASYLX010000000
VERSION     DASYLX010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282230"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGP27_25125"
     CDS             complement(<1..365)
                     /locus_tag="VGP27_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854618.1"
                     /translation="MAPRTRGAQPISKTDIDVYYDPYDVALNADPYPMFRRIREEAPL
                     YFNDRHGFYALSRYDDVDAAYHDHETFSSARGAVLEIIQSGMDIPSGTLIFEDPPIHN
                     IHRKLLSRIFTPRKVAALEP"
     gene            438..1889
                     /locus_tag="VGP27_25130"
     CDS             438..1889
                     /locus_tag="VGP27_25130"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7854619.1"
                     /translation="MSDIALQPAHALAAALRRRELSSRELLEHYLSRVEAFNAPLTAV
                     VTLDPDGALAAADAADASLARGDEVGPLHGVPMTVKDTYLTAGVRTTCGLPAWDHVPE
                     HDAEAVQRLRGAGAVIFGKTNTPALAGDWQTYNAIFGTSNNPWDTTRSTGGSSGGAAA
                     AVATGMTGLELGSDIGGSIRFPANWCGVCGHKSTWGIVSQAGHLPPAPGTLSSTDLSV
                     VGPLARDVADLELALGVLAGPAGQAAVGWRLELPAPRATTLGDVRFALWLDDPAYPVE
                     AEVEAVLQAAADALRNSGAHMVDARPEVVLPDVVRLYQQFLYPILLSTMGKRSFDNMV
                     GLAESLADGDDRPLARTARFATQRYRDWVFAGERREQLRALMADFFREVDVLLMPVSM
                     VPAIPHDHSEAFPERVIQVDGVVRPYTDLMAWVALATLTHLPATVVPVGRTPSGLPVG
                     VQIVGPYLEDRTTLAVGRLIEDLLGGFTPPPGY"
     gene            complement(1921..2358)
                     /locus_tag="VGP27_25135"
     CDS             complement(1921..2358)
                     /locus_tag="VGP27_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854620.1"
                     /translation="MKSVMVMSFVAALSAVASLLAPPAQAMMQPGNYDVLTNRYTQAS
                     WVWFVSNCQPTAPDCVDVSAIPRLKFYAYYDGDAHLVDGRYTLTVDVNDGLRCLPGYS
                     MPTRDTYTWDPVTLAGEINSTYDVGCFGGPPGTQFWTFALQRL"
     gene            complement(2426..3790)
                     /locus_tag="VGP27_25140"
     CDS             complement(2426..3790)
                     /locus_tag="VGP27_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854621.1"
                     /translation="MTVDDISAADSARKKNRYHFDRHTPEYRLRFDEITEEMQAKCPM
                     AWTDVYNGHWVAADSKHVFELARCPAVSNHHDLSGETPYQGITIPKAQRATVVRGGIL
                     EMDEPEHSLYRGTLNPYLSPAAIKRWQPFVDEIVRAALDEKIETGRIDFVDDMANIVP
                     AVLTLAMMGIELRKWPVYSEPAHLSVSTPEHSPDAARVAEMNRQMGIDMITTMTEIRE
                     NPRPGLVNALLQLRIDGEPAPDLEILGNLGLIIGGGFDTTTALTAHSLEWLSEHPDQR
                     ELLSRERDTLLNPATEEFLRFFTPAPGDGRTFSEDVEVEGTRFKEGERLWISWAMANR
                     DPAVFEKPNELVMDRKGNRHFSFGIGVHRCVGSNVARTVFKSMLTAVLDRMPDYVCDP
                     DGTEHYETIGVIQGMKHLPATFTPGPKLGPGLEETLEKLQRICDEQELARPITERKEA
                     AVID"
     gene            complement(3799..3993)
                     /locus_tag="VGP27_25145"
     CDS             complement(3799..3993)
                     /locus_tag="VGP27_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7854622.1"
                     /translation="MKVSVDSERCQGHTLCGMIAPDSFQLSDIDGTASPVNDVVPADQ
                     EDAVREAVHSCPEQAIAIEE"
     gene            complement(4006..4866)
                     /locus_tag="VGP27_25150"
     CDS             complement(4006..4866)
                     /locus_tag="VGP27_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854623.1"
                     /translation="MERVQGKVAFVTGAGRGQGRSHAVRLAEEGADIIAVDRCEDFAT
                     IGYPMATPEDLEETAQFVEKTGQRCVAAQVDVSDAGALKSALEEGIAELGRLDIVVTA
                     AGIAGMKGSADMQAWIDVINTNLIGTINAIQVALPHLGEGASIVATGSTAALMDVAKN
                     DNPGTDPGGSAYLHSKRLLSQYVHNLAAELAPRGIRANVVHPTNTNTPMLQSAPMYKS
                     FRPDLENPTRADAEPVFHVQQAMKVPWVEPEDISNMVLFLASDEARYITGTQQRVDAG
                     GYLKWYDYHV"
     gene            complement(4888..5592)
                     /locus_tag="VGP27_25155"
     CDS             complement(4888..5592)
                     /locus_tag="VGP27_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854624.1"
                     /translation="MKTAVVTGGGSGIGQAVANRLRSDGYHVATLDLVASDEKFAHTA
                     DVTDRAAVDAALTAVREQFGPVTILVNAAGMERFKRFADLTFDDFHRVVDVNLNGVFH
                     CVQAVLPDMIDAAWGRIVNISSSSAQSGQPYMSAYVAAKSAVNGLTKSLALEYGPMGI
                     TVNAVPPGFIDTPMLRKSEERGRLGGTVEEHIARTPVRRVGKPEDIAAACAFLISEEA
                     GYITGQILGVNGGRNT"
     gene            5835..7334
                     /locus_tag="VGP27_25160"
     CDS             5835..7334
                     /locus_tag="VGP27_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854625.1"
                     /translation="MTEASTISGGPGTTADPSVRAADRRMLIDGQLVDTPRTFPSINP
                     ATEEVVGYAPDATVADAEAAVAAARRAFDTTDWSTNTELRIRCLDQLHQALVDHRDEL
                     VALTIADVGATAAMCAGAQLDAPISIVRYYADLLKTYPLTEDLGNSESHGMQHHRWVE
                     KEAAGVVAAIIAYNYPTQLALAKLAPALAAGCTVVLKGAPDTPLITLALGELIAEHTD
                     IPAGVVNVLSGTAPEVGIALTTSPDVDMITFTGSTPTGRAIMAAASETCKRVFLELGG
                     KSAAVVLDDGDFDMAALFTAFATASHSGQGCAITTRILVPSKHHDEIVEKLKNNFGVV
                     SYGNPAEEGVYTGPLISERQRDKVDGMVRRAVEAGATLVAGGEKVSPGFFYTPTLLTD
                     VDADSEIAQHEVFGPVLVVIRYEDDDDAVRIANNSIYGLSGAVFGSEDRALAVARRIR
                     TGTLSINGGMFFAPDSPFGGYKQSGIGREMGAAGLEEFLERKTIATVVS"
     gene            7396..>7917
                     /locus_tag="VGP27_25165"
     CDS             7396..>7917
                     /locus_tag="VGP27_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854626.1"
                     /translation="MSTAPLDGIRVLVVAMYGFVPSAGAVLGEWGADVIKVEHAVTGD
                     PQRGLRQTGPLRVEGDPNPNIEHANRGKRSIGLDMSVPEGREVVYELARRADVFLTSF
                     LPGARQKFGIDVDDIRAVNPKIIYARGSALGPRGQEATKGGYDMTAFWCRASTAASIT
                     PPDHDGMIGPPAPA"
BASE COUNT         1282 a         2558 c         2659 g         1418 t
ORIGIN      
        1 ggttccagcg cggcaacctt gcgcggcgtg aagatccgcg acagcagctt gcggtggatg
       61 ttgtggatcg gcggatcctc gaagatgagg gtgcccgaag ggatgtccat gccggactgg
      121 atgatctcca gcaccgcacc acgagctgag ctgaacgtct cgtggtcgtg gtatgcggcg
      181 tcgacatcgt cgtagcggct cagcgcgtag aagccgtggc ggtcgttgaa gtagagcggc
      241 gcctcctcgc ggatccggcg aaacatcgga tacgggtcgg cgttgagcgc gacgtcatat
      301 gggtcgtagt agacgtcgat gtcggtcttc gagattggtt gtgctcctcg cgtgcgcggt
      361 gccacgaagc aagtctttca gcgcccgaac gtattcgggc agtttgtact ctttgcttaa
      421 aagaaaggtc gttctgcatg tccgacatcg ctttacagcc tgcacatgcg ctggcagcgg
      481 cccttcgtcg ccgcgaactg tccagtcgcg agttgctgga gcactatctg agccgcgtcg
      541 aagcattcaa cgcaccgctg acggccgtcg tcacgctcga cccggacggc gcgctggcgg
      601 cggccgacgc agcggatgcg tcgctggctc gcggtgacga ggtcgggccg ctgcacggcg
      661 tgccgatgac cgtgaaggac acctacctga ccgcgggggt gcggaccaca tgcggcctgc
      721 ccgcatggga ccacgtgccg gagcacgacg cggaggccgt ccagcggctg cgcggtgcgg
      781 gcgcggtgat cttcggcaag accaacaccc cggcgctggc gggcgactgg cagacctaca
      841 acgcgatctt cggcaccagc aacaacccct gggacacgac gcgatccacc ggcgggtcgt
      901 cggggggtgc cgcggccgcg gtcgcgacgg gtatgacggg cctcgagctg ggcagcgaca
      961 tcggcggctc gatccgcttc ccggccaact ggtgcggggt atgcggccac aagtcgacat
     1021 gggggatcgt ctcgcaggcg ggtcacctgc cgcccgctcc cggaaccttg tcgagcaccg
     1081 atctctccgt cgtcgggccg ctggcgcgtg acgtcgccga tctcgagctg gcgctcggcg
     1141 tgctcgcggg gccggccggg caggccgcgg tggggtggcg gctcgagttg cctgctccgc
     1201 gggcgacgac gctgggcgac gtgcggttcg cgctttggct cgacgacccg gcctatcccg
     1261 tcgaagccga ggtggaggcg gtgctgcagg cggccgccga tgcgctgcgg aactccggcg
     1321 cgcacatggt cgacgccaga cccgaggttg tgctgcccga tgtggtccgg ctgtatcagc
     1381 agttcctgta cccgatcctg ctgtccacca tgggcaagcg gtcattcgac aacatggtcg
     1441 gcctcgccga gtctctcgct gacggcgatg accgcccgct ggcccgcacc gcccgcttcg
     1501 ccacgcagcg ctaccgagac tgggtgttcg ccggcgagcg acgcgagcag ttgcgtgcat
     1561 tgatggcgga cttcttccgc gaggtagatg tgctgctgat gccggtgtcg atggtgcccg
     1621 ccatcccgca cgaccacagc gaggcgttcc ccgaacgggt gatccaggtc gacggcgtcg
     1681 tgcggcccta cacggatctg atggcgtggg tggcgttggc aacgttgact catctgcccg
     1741 cgaccgtcgt ccccgtcggc cgcaccccgt cgggcctgcc ggtcggcgtc cagatcgtcg
     1801 ggccctatct cgaggaccgc actacgctgg ccgtcggccg tctgatcgaa gacctgctcg
     1861 gcgggttcac cccgccaccg ggttactagc gagcgtgcgc aaaccccctt gcacgcggag
     1921 ttacaggcgc tgcagtgcga aggtccagaa ctgggttccc ggcggcccgc cgaagcagcc
     1981 gacgtcgtag gtggagttga tctcaccggc cagcgtgaca gggtcccagg tgtaggtgtc
     2041 gcgggtgggc atgctgtaac caggcagaca gcgcaagcca tcgttgacgt ccacggtcaa
     2101 cgtgtagcgg ccgtcgacaa ggtgcgcgtc gccgtcgtag taggcataga acttcaatct
     2161 cggtatcgcg ctgacgtcca cgcaatccgg ggccgtgggc tgacagttcg acacgaacca
     2221 tacccacgaa gcttgcgtgt acctgttcgt cagcacatcg taattcccag gctgcatcat
     2281 ggcctgcgct ggcggggcaa gcagcgacgc gaccgcggat agggccgcga cgaaactcat
     2341 gaccatgaca gacttcatgg cccctccatc catctcatcc cgacggctgc ctatcaaatc
     2401 acgtcgccac acattttatt tggtatcaat cgattaccgc ggcttccttg cgctcggtga
     2461 tcggccgggc gagctcctgt tcgtcgcaga tccgctgcag tttctcgagt gtctcctcca
     2521 gtccggggcc gagtttgggc cccggggtga aggttgcggg taaatgcttc atgccctgga
     2581 tgacgccgat ggtctcgtag tgctcggtgc cgtcggggtc gcagacgtag tcgggcatcc
     2641 ggtcgagcac ggcggtcaac atcgacttga acaccgtcct ggccacgttg gaaccgacac
     2701 aacggtgcac gccgatgccg aagctgaagt gccggttgcc ctttcggtcc attaccagct
     2761 cgttgggctt ttcgaacacc gcggggtcac gattggccat cgcccacgag atccagagcc
     2821 gctcgccttc cttgaacctc gtcccctcga cctcgacgtc ctcggagaac gtgcggccgt
     2881 cgccgggcgc gggcgtgaag aagcgcagga actcctcggt ggcagggttg agcagcgtgt
     2941 cccgctcgcg gctgagcaac tcgcgctgat ccggatgctc cgagagccat tccaacgaat
     3001 gtgcggtcag cgccgttgtg gtgtcgaacc cgccgccgat gatgagcccg aggttgccca
     3061 ggatctccag atccggggcc ggttcgccgt cgatgcgcag ttggagaagc gcgttcacca
     3121 gaccgggtcg cgggttctcc cgtatctcgg tcatcgtggt gatcatgtct atgcccatct
     3181 gccggttcat ttcggcgacg cgcgcggcgt cgggcgagtg ctccggggtc gacaccgaga
     3241 ggtgcgcggg ctcgctgtag accggccact tccgcaattc gatgcccatc atcgccagcg
     3301 tcagcacggc gggcacgatg ttggccatgt cgtcgacgaa gtcgattcgg cccgtctcga
     3361 tcttctcgtc gagggctgcg cgcacgatct cgtcgacgaa cggctgccac cgtttgatgg
     3421 cggcggggga gagatacggg ttcagcgtcc cccggtagag gctgtgttcg ggctcgtcca
     3481 tctccaggat gccgccgcgt acgacagtgg cccgctgcgc cttggggatc gtgatgccct
     3541 ggtagggagt ctcgccgctg aggtcgtgat gattggagac cgccggacag cgcgcgagtt
     3601 cgaagacgtg cttgctgtcc gctgcgaccc agtgtccgtt gtagacgtcg gtccaggcca
     3661 tcgggcactt ggcctgcatt tcctcggtga tctcatcgaa ccgcaggcgg tactccggtg
     3721 tgtgccggtc gaagtggtac cgattcttct tgcgggcgct gtcggcggcg cttatgtcgt
     3781 cgacggtcac tgtctacctc actcctcgat cgcgatggcc tgctcagggc acgagtgcac
     3841 ggcttcgcgc accgcgtcct cctggtcggc gggaacgacg tcgttcaccg gcgacgcggt
     3901 gccgtcaatg tcgctgagct ggaacgaatc cggcgcgatc atcccgcaca gcgtgtgacc
     3961 ctggcagcgc tccgaatcga cagaaacctt cacttctgac ctccgttaga cgtggtagtc
     4021 gtaccacttc aaatagccgc ctgcgtcgac gcgctgctgc gtgcccgtga tgtagcgggc
     4081 ctcgtcggag gccaggaaca gcaccatgtt gctgatgtcc tcgggctcga cccacggcac
     4141 cttcatcgcc tgctggacgt ggaacaccgg ctcggcgtcg gcccgcgtcg ggttctccag
     4201 gtcgggccgg aacgacttat acatcggcgc gctctgcagc atcggggtgt tcgtgttcgt
     4261 cgggtgcacg acgttggcgc ggatgccccg cggtgcgagc tcggccgcga ggttgtgcac
     4321 gtactgcgac agcagcctct tcgaatgcag gtaagcggag ccaccggggt cggtgccggg
     4381 attgtcgttc ttggcgacgt ccatcagcgc ggcggtggag ccggtggcca cgatcgatgc
     4441 gccctcgccg aggtgtggca gcgcgacctg gatggcgttg atggtgccga tcaggttggt
     4501 gttgatcacg tcgatccagg cctgcatgtc cgccgacccc ttcatgccgg cgatgccggc
     4561 cgcggtgacg acgatgtcga gcctgcccag ctcggcgatg ccctcctcga gcgccgactt
     4621 cagcgcgcct gcgtcgctga cgtcgacctg cgccgccacg cagcgctgcc ccgtcttctc
     4681 gacgaactgt gcggtctcct cgaggtcctc cggggtcgcc atcggatagc cgatcgtcgc
     4741 gaagtcctca catcgatcga cggcgatgat gtcggcgccc tcctcggcca gccggaccgc
     4801 gtggctgcgg ccctggccgc gcccggcgcc ggtgacgaag gcgacctttc cctgtacgcg
     4861 ttccactaga agatgtcctt tcgctcgtta ggtgtttcgg ccgccgttga ccccgaggat
     4921 ttggccggtg atgtagccgg cctcctcaga gatcaggaag gcgcatgcgg cggcgatgtc
     4981 ttccggtttg ccgacccttc gcaccggcgt gcgggcgatg tgttcctcga cggttccccc
     5041 gagccgcccg cgttcttccg acttgcgcag catcggagtg tcgatgaaac ccggcggcac
     5101 cgcgttgacc gtgatgccca tcggcccgta ttcgagggcc agcgatttgg taaggccgtt
     5161 taccgcagat ttagccgcaa catacgcgga catgtacggc tgccccgatt gcgcgctgga
     5221 cgaggagatg ttgacgatgc gtccccaggc ggcgtcgatc atgtccggta ggaccgcctg
     5281 cacgcagtgg aaaacgccgt tgaggttcac gtcgacgacg cgatggaagt cgtcgaacgt
     5341 gaggtcggcg aaccgcttga aacgttccat cccggcggcg ttgaccagga tggtgaccgg
     5401 accgaactgc tcgcggactg cggtcagcgc ggcgtcgacc gctgcgcggt cggtgacgtc
     5461 ggcggtatgc gcgaacttct catcggacgc gacgaggtcg agcgtggcga cgtgataacc
     5521 gtcggatcgc agccgattgg cgacagcctg accgatgccg gagccgcctc cggtgaccac
     5581 ggcggtcttc acggcagcgc gccggtctca gacatcaaga atcttccttc cgattatgag
     5641 aaccgtactc tcgcattgtg cggcatcgca agacaaatcg gccgtaattg tcgcatccga
     5701 ttttgtgtga acgtcgctat caggctagtt gactaggcca tcttcgtttt cttgagttct
     5761 catcgcgcga atgtatgatt cgccgaggat gagaatgaag tttccatcac actgaacttt
     5821 gcggaggagg gtggatgacg gaagcgtcga cgatctcggg tgggccgggc accactgcag
     5881 acccatcggt acgtgctgcc gaccggcgga tgctgatcga cggccaactg gtcgacacac
     5941 cccggacgtt cccttctatc aaccccgcca cagaagaggt ggtcggttac gccccggacg
     6001 cgacggtcgc cgacgccgag gccgcggtcg cagcggcacg gcgggcgttc gacaccaccg
     6061 actggtccac caacaccgag ctgcggatcc gctgcctcga tcagttgcac caggcattgg
     6121 tcgaccatcg cgatgagctc gtcgcgctga ccatcgccga cgtcggggcg acggcggcga
     6181 tgtgcgcggg cgcgcagctc gacgcgccga tctcgatcgt gcgctattac gcagacctgc
     6241 tgaagacgta cccgttgacc gaggatctcg gcaacagcga aagccacggt atgcagcacc
     6301 accgctgggt ggagaaagag gccgccgggg tggtggcggc gatcatcgcc tacaactacc
     6361 cgacccagct cgcgctggcg aaacttgcgc cggccctggc ggccgggtgc acggtcgtat
     6421 tgaagggcgc accggatacg ccgttgatca cgctcgcgct cggcgagctg atcgccgaac
     6481 acaccgacat cccggccggc gtggtcaatg tgctgagcgg caccgctccc gaagtgggta
     6541 tcgccctgac gacgagcccc gacgtcgaca tgatcacctt caccggttcc acgccaacgg
     6601 gtcgggcgat catggctgcc gccagcgaga cgtgcaaacg cgtcttcctc gaactcggcg
     6661 gaaagtcggc ggccgtcgtg ctcgacgacg gcgattttga catggccgcg ctgttcaccg
     6721 cgttcgccac cgccagccac tccggccagg gctgtgccat cacgacccga attttggtac
     6781 cgagcaagca ccacgacgag atcgtggaga agttgaagaa caacttcggc gtcgtgtcgt
     6841 acggcaaccc cgccgaggag ggcgtctaca cgggtccgtt gatcagcgag aggcagcgcg
     6901 acaaggtcga cggcatggtc aggcgtgccg tcgaggccgg cgcgacgctg gttgccggcg
     6961 gcgagaaagt gagtccgggc ttcttctaca caccgacact gctcaccgac gtcgacgccg
     7021 acagcgagat cgcccagcac gaggtgttcg ggccggtgct cgtcgtcatc cgctacgaag
     7081 acgacgacga cgccgtccgg atcgccaaca actccatcta cggattgtcg ggcgccgtgt
     7141 tcggcagcga ggaccgcgcc ctcgcggtgg ctcgccgcat ccgcaccggc acactgtcga
     7201 tcaacggcgg catgttcttc gcacccgaca gtccgttcgg cggctataaa cagtccggca
     7261 tcggcaggga gatgggcgcc gcggggctcg aagagttcct ggagcgcaag acgattgcga
     7321 cggtggtctc atgagccccc gaagacgagc gcgagcgagg aacgagcgag gaccggagcg
     7381 agtgggagtc gaggcatgag cacagcacct ctggacggga tccgcgtgct cgtggtcgcc
     7441 atgtacggct tcgttccttc ggcgggtgcg gtgcttggcg agtggggcgc cgacgtcatc
     7501 aaggtcgagc acgccgtcac cggtgacccg cagcgtgggc tgcgccagac cggaccgctg
     7561 cgcgtcgagg gcgatcccaa cccgaacatc gagcacgcca accgcggcaa gcgcagcatc
     7621 gggctggaca tgtcggtgcc cgagggtagg gaagtggtct acgagctggc ccgccgcgcc
     7681 gacgtcttcc tgacgagttt tctgccgggc gcccggcaga agttcggcat cgacgtcgac
     7741 gacatccggg cggtcaaccc gaagatcatc tacgcccgcg ggagcgcgct cggcccgcgc
     7801 ggtcaggagg cgaccaaagg cggctacgac atgaccgcgt tctggtgccg cgcctcgacc
     7861 gcggccagca tcacgccgcc ggaccacgac ggcatgatcg ggccgccggc gcccgcg
//
LOCUS       DASYLX010001075         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1940741,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001075 DASYLX010000000
VERSION     DASYLX010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940741"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGP27_25170"
     CDS             complement(<1..368)
                     /locus_tag="VGP27_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854627.1"
                     /translation="MSHYKSNVRDQVFNLFEVFGLDKALGQGEYADLDADTAREMLGE
                     IARLAEGPVAESFADSDRNPPTFDPKTFSVTLPEPFKKSVRAMLDGGWDRVGLDEELG
                     GVPAPKALMWALHEHILGANP"
     gene            615..1703
                     /locus_tag="VGP27_25175"
     CDS             615..1703
                     /locus_tag="VGP27_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEV7854628.1"
                     /translation="MQQRVVVWGTGNMGSTAIRSALAFPGLGLCGIITSSDSKEGMDA
                     AEFVGLDSPTGVAATTDVDAALADCDAVAYMASGDIRPDEAVNDIERCLRAGRHVVTP
                     SLYSLYDPRSAPPEWVERLTAAAEEGASTLLVSGVDPGWGNDALAVIAAGLCTRIRSI
                     HCQEIFDYSTYNQPHAVKVLCGFGGSMDETPMMLLPSIPTMVWGGNLRLIGRGLGLEI
                     EEITEDVQRRPLETPVDTVMGRFDEGTQGAFWLKVIGKAGGRERIVIDHITRIHPSCA
                     PDWPQPDQGAGDHRVIVHGDPELTLVSRADVPGGTRADGGNTTAANRLLGAIGWLATQ
                     KPGIYDGLDVPIHPPLPPEVEAQRWTSD"
     gene            complement(1700..>2138)
                     /locus_tag="VGP27_25180"
     CDS             complement(1700..>2138)
                     /locus_tag="VGP27_25180"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV7854629.1"
                     /translation="IKANWQRAAKVLGSSRPPILDLVLPSAVGEVTGDSELLRQLQEV
                     PVAQRAGFVTEFLQREVQNFLRLAQPPAATSRFLDLGTDSLMAIELRNRLHSQFGGAF
                     TINATAVFDYPTIGGLAEYLVGQLPDAESPPQAGEADSVASAD"
BASE COUNT          377 a          696 c          718 g          347 t
ORIGIN      
        1 gggttggcgc cgaggatgtg ctcgtgcagc gcccacatga gcgccttggg cgcgggcacg
       61 ccgccgagtt cctcgtccag gccgacgcgg tcccagccgc cgtcgagcat ggcgcgcacc
      121 gacttcttga acggctcggg cagcgtgacc gaaaacgtct tgggatcgaa ggtcggggga
      181 ttgcggtcac tgtcggcgaa cgattcggcg acgggaccct cggccagccg ggcgatctcg
      241 ccaagcatct cgcgcgcggt gtcggcgtcg agatctgcgt actcgccctg gccgagcgcc
      301 ttgtcgaggc cgaacacctc gaataggttg aatacctggt cacggacgtt gctcttgtag
      361 tggctcacgt tgtcctcctc gatgagaatg ccacatcggt tgggtacttt tggctaagtt
      421 acccaccagt aactacggct caatataccc gctggtaact tcaacgcaaa gccgccggga
      481 gcaatttcca tgagctaagt aaccaggcgg ttggtcgggc cccgatctac cggccaaaac
      541 gctgaatgag ctggggatac gccagcttgt gatacttacc acagagtcgt cacacccacc
      601 actaaagtga acgaatgcaa cagcgcgtgg tggtgtgggg gacgggcaat atggggtcga
      661 cggccatccg gtcggcgctg gcatttcccg gtctggggct gtgcggcatc atcacctcgt
      721 ccgattccaa agagggcatg gacgcggccg agttcgtcgg cctcgacagc ccgacgggag
      781 tcgcggccac gaccgacgtc gacgctgccc tcgccgactg cgacgccgtc gcctacatgg
      841 cctcgggcga catccggccc gatgaggccg tcaatgacat cgagcgctgc ctgcgcgcgg
      901 gcaggcacgt cgtcacgccg tcgctgtact cgctctacga cccgcggtcc gctccgcccg
      961 aatgggtcga gcggctgacc gcggccgcgg aagagggtgc gtcgacgctg ctcgtcagtg
     1021 gggtggaccc gggatgggga aacgacgcac tcgcggtgat cgccgcgggc ctgtgcacca
     1081 ggatccggtc gattcactgc caggagatct tcgactactc gacctacaac cagccgcacg
     1141 cggtgaaggt gttgtgcggg ttcggcggct cgatggacga gacgccgatg atgctgctgc
     1201 cgtcgatacc aaccatggtg tggggcggca atctccggct gatcggccgt ggtctcggcc
     1261 tcgagatcga ggagatcacc gaagacgtgc agcgccggcc gctggaaacg ccggtcgaca
     1321 ccgtgatggg ccggttcgac gagggcaccc agggtgcctt ctggctgaag gtgatcggaa
     1381 aggcgggcgg gcgcgagcgc atcgtcatcg accacatcac ccgcatccac ccctcgtgcg
     1441 caccggactg gccgcagccc gaccaggggg cgggcgacca ccgcgtcatc gtgcacggcg
     1501 atcccgagct caccctggtc agcagggccg acgtgccggg tggcacgcgg gcggacggcg
     1561 gcaacaccac ggccgcgaac cggctactcg gcgccatcgg atggctggcg acgcagaagc
     1621 cgggcatcta cgacggactc gacgtgccca tccacccgcc gctgcctccc gaagtggagg
     1681 cacagcggtg gaccagcgat tagtcggcgc tcgcgacgga atccgcctca cccgcctgcg
     1741 gcggcgattc cgcgtcgggc agctgtccca cgagatactc ggcgaggccc ccgatggtcg
     1801 gatagtcgaa taccgcggtc gcgttgatcg tgaacgcgcc gccgaactga ctgtgcaacc
     1861 ggttgcggag ttcgatcgcc atcaatgaat ccgtgcccag gtcgaggaac cggctggttg
     1921 cggcgggcgg ttgcgcgagc cgcaggaagt tctgcacttc gcgctgaagg aattcggtca
     1981 cgaaaccggc gcgctgtgcc accggcacct cctgcagctg ccgaagcaac tcgctgtcac
     2041 cggtcacctc cccgacggca ctgggcaaca cgaggtcgag aatcggtgga cgagaactgc
     2101 caagcacctt tgccgcacgc tgccaattgg ccttgatg
//
LOCUS       DASYLX010001076         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2269447,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001076 DASYLX010000000
VERSION     DASYLX010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269447"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..462
                     /locus_tag="VGP27_25185"
     CDS             10..462
                     /locus_tag="VGP27_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7854630.1"
                     /translation="MILDDVQLPRQRVIRGAGVVMAAGALAACSKTEEKPAATAESAT
                     TSADSAPAGTAAPGGEAIAKTADVPVGSGVIVDQVVITQPVAGEFKGFSSKCTHKGCA
                     VNKVADGTIDCPCHGSKFNLDGTVAKGPATEPLETKAIAVQGDSIVLA"
     gene            complement(470..>1506)
                     /locus_tag="VGP27_25190"
     CDS             complement(470..>1506)
                     /locus_tag="VGP27_25190"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7854631.1"
                     /translation="GADDAAMDAALLALAVPGRHVQVVWSRADELSWAPLGTAGVVRI
                     AAGTASDGTVSSWRHEIWSGTFISRPGMTPPPAFLSTSLRDRAAIHSNAEPPLERGGG
                     TRRNAVPGYDFPSYEVINHLIPAMPLRTSALRSLGAHLNIFAAESFMDELATDAGVDA
                     IEYRLAHLADPRGRAVLEAVARSSNWGQHTRSDSIGHGVGYARYKNSSAYCAVIAEVE
                     AVEQVRVRRLSIAVDAGLVISPDGATNQIEGGAIQATSWTVKERVRFDRYNVTSDNWE
                     TYPILRFSEVPAVDVELMPGDGHQPLGVGECAQGPTTAAIANALCDAIGVRVRSLPLT
                     PDQIVAAIPD"
BASE COUNT          246 a          492 c          535 g          233 t
ORIGIN      
        1 ggttccagca tgattcttga cgatgttcag ttgccgcggc agcgggtcat tcgcggcgca
       61 ggcgtcgtga tggccgcagg cgcactcgcg gcctgctcga aaacggagga gaagcccgcg
      121 gcgaccgccg aatcggcgac caccagcgcg gactccgccc cggcagggac agccgcgccg
      181 ggcggcgaag cgatcgccaa gaccgccgac gtaccggtcg gctccggcgt gatcgtcgac
      241 caggtcgtca tcacccagcc ggtcgcaggc gagttcaaag gcttctcgag caaatgcacg
      301 cacaagggat gcgcggtgaa caaggtggcc gacgggacga tcgactgccc ctgtcacggg
      361 agcaaattca acctggacgg cacggtcgcc aagggcccgg cgacggaacc gttggagacc
      421 aaggccattg cggtgcaggg cgattcgatc gtcctggcgt gaccggcggt cagtccggta
      481 tcgccgcaac gatctgatcc ggggtgagtg gtagcgatcg gacgcgaaca ccgatcgcgt
      541 cgcagagcgc gttggcgatg gccgcagtcg tcggcccctg cgcgcattca ccgacgccga
      601 gcggttggtg accgtcgccg ggcatgagtt cgacgtcgac ggcgggcacc tcggagaacc
      661 gcaggatcgg ataggtctcc cagttgtcgc tcgtgacgtt gtaccggtcg aaccggacgc
      721 gctctttgac cgtccagctg gtggcctgga tcgcgccgcc ctcgatctgg ttggttgcac
      781 cgtcggggct gatgacgagg ccggcgtcga cggcgatcga cagccggcgc acgcgtacct
      841 gctcgacggc ctccacctct gcgatgacgg cgcaataggc actcgaattc ttgtagcgcg
      901 cgtagccgac gccgtgaccg atcgagtctg accgcgtgtg ctggccccaa ttcgagctcc
      961 gggccacggc ttccagcacg gcgcggccgc gcgggtcggc cagatgcgca agccggtatt
     1021 cgatggcgtc gacgccggcg tcggtagcga gttcgtccat gaacgattcc gcagcgaaga
     1081 tgttgaggtg cgcgccgagg gatctcagcg ccgacgtccg caatggcatc gcaggtatca
     1141 gatggttgat gacttcgtag gacggaaagt cgtacccggg aacggcgttg cggcgagtgc
     1201 caccgccgcg ctcgagcggt ggttcggcat tggagtggat tgccgcgcgg tcgcgcaggc
     1261 tggtggaaag gaatgccggc ggcggcgtca tgccggggcg gctgatgaag gtgccgctcc
     1321 agatctcgtg ccgccacgac gacaccgtcc cgtcgcttgc ggtgccggcc gcgatccgga
     1381 cgacgcccgc cgtccccagc ggcgcccacg acagttcgtc ggcgcgcgac cacaccacct
     1441 ggacgtgcct gcccggcacg gcaagtgcca gcagtgccgc gtccatcgcg gcgtcgtcgg
     1501 ccccgt
//
LOCUS       DASYLX010001077         7230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1614533,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001077 DASYLX010000000
VERSION     DASYLX010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614533"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1486
                     /locus_tag="VGP27_25195"
     CDS             65..1486
                     /locus_tag="VGP27_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEV7854632.1"
                     /translation="MDGTAPASVHTVLADADGNAYRSLRQRPVTRAERYALGKSLRKR
                     VPRKSLADWTVLSDRPDPVELINISHRGRVDRLIPIRVGRMVDSPYGFLRGTAVVMAE
                     DVAHLPATGITPVVCGDSHFGNFGFYASPERDLVIDLNDFDEAHPGGWEWDLRRLAAS
                     IWVAGRHNGTSEDHCGNAVRSCVASYRQELRRLADLPLFTRSFVRLDVDRLAAETAGP
                     LSEQVVRSAKRARQRTGDRALPRFTHEVDGARRIVEEPPLITRLPPREADLLAQALDE
                     YLETLSSYWRRVLGGYTLVDVAHKVVGVGSVGLRAYVALLEGSSESDVVFLQLKQARR
                     SVLGRYVHGESAWHAHQGQRVVEYQQSLQTVSDPLLGWTTMDGRQYYVRQFRNMKGRI
                     PLDAIDAAALTDYAGVVGQLLAKGHARTSGASMIAGYMGSSDRVDKALCKFARRYADQ
                     TEADHAALVRAVDRGLLPVERGV"
     gene            1498..1950
                     /locus_tag="VGP27_25200"
     CDS             1498..1950
                     /locus_tag="VGP27_25200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7854633.1"
                     /translation="MQVRRAGPSDVAVIEHIVHAAFGIYVARIGRQPAPMTADYRAAV
                     ASSRVWVIDADGEVAGVVVNQVHDDHLLLDTVAVAPGAQGHGYGALLLARAEDDAREL
                     GLAEVRLVTNQAMTENQTFYPRHGYIETGCGRQDGYHRVFYAKNVAAQ"
     gene            complement(1988..2338)
                     /locus_tag="VGP27_25205"
     CDS             complement(1988..2338)
                     /locus_tag="VGP27_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV7854634.1"
                     /translation="MHETDDPYTVLGLTPTATRAEITHAYRSRLRALHPDTRRRATPG
                     LTEASDADLQRVVAAYTRLRTAAHRDGRDHAEPVSCTPSRARPVRIPIIYRHSQMPGA
                     SRPPLWAGPVREHD"
     gene            complement(3042..3461)
                     /locus_tag="VGP27_25210"
     CDS             complement(3042..3461)
                     /locus_tag="VGP27_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEV7854635.1"
                     /translation="MLLRTDPFRDLDRFAQQVWGTAARPAVMPMDAWREGDQFIVEFD
                     LPGVNGDSLGLDVERNVLTVHAERSAPDPNREMVSAERPRGVFSRQVFLGDNLDADRI
                     EASYHDGVLQLRIPVAEKAKPRRIEISHNSEKKPINA"
     gene            3585..4040
                     /locus_tag="VGP27_25215"
     CDS             3585..4040
                     /locus_tag="VGP27_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7854636.1"
                     /translation="MADDATLRSARGVYGISVTAELSGVDPQTLRLYERRGLITPART
                     DGGTRRYSDDDVARVARITELVDAGVNLTGIGHILDLQDRNTRLEADNARLSPNADQS
                     AGRQGSGDRNEIRGLRSGAQLPGHSPVHRWNSRAPWRSMTVLTTEKIAP"
     gene            complement(3947..4240)
                     /locus_tag="VGP27_25220"
     CDS             complement(3947..4240)
                     /locus_tag="VGP27_25220"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEV7854637.1"
                     /translation="MAHQKSSTSAKILYRPVGLISSVLGGLLAGVIFRQVWQRAAPGD
                     KPDPPKALETEYPLKEILIAAAIQGAIFSVVKTVIDRQGARLFQRWTGEWPGS"
     gene            complement(4313..4849)
                     /locus_tag="VGP27_25225"
     CDS             complement(4313..4849)
                     /locus_tag="VGP27_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV7854638.1"
                     /translation="MSQMTQDGTGAAFGDRAQLESVISADLRALNAQADRIGRYFADM
                     HGLSATEFHALLHIMVGETSRSPLTSGELRQRMGLTNAGVTYLVRRMIAAGHIRRDTD
                     DADRRKVILRYQPHGMELARSFFTHLGAANRAAIAGFTDRELETAHQVMSTLTTAMDH
                     FHNQLNEGGPGSSDETTA"
     gene            4948..5703
                     /locus_tag="VGP27_25230"
     CDS             4948..5703
                     /locus_tag="VGP27_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEV7854639.1"
                     /translation="MSGERPDVLDRLDAAAIAVASLNALLVADGSLDDVLHGVALNAV
                     RAVPGADAVSITLVDLPLRTVAHTDDDILVLDQEQYARRQGPCLQAAETRRPVRAAMG
                     PGERRWPEFVSAARAAGVRATLSIPLIIAPAVPGGDDELVGSLNAYSRSTAAFDVVDE
                     KLMRLYTDAAGQAVTDARRWQRLRETVGQLEEALVSRADIDQAKGVLRTQHGGTADEA
                     FARLVERSQRENVKIRDIARRILEGLPRRNCNG"
     gene            complement(5916..6695)
                     /locus_tag="VGP27_25235"
     CDS             complement(5916..6695)
                     /locus_tag="VGP27_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854640.1"
                     /translation="MEPLLLLHGVMMSAAAWADVVPLMTDYHEIIAPNAIGHRGGPRL
                     TGSASIAALTDQTERLLDARGLGRVHIAGNSMGGWMAIELARRGRALTVCAFSPAGFW
                     TPGASDETHATNMVRRVRRRARLVRPLAPALLRIGQLRRLAMRDSAEHGDRLTAAQAL
                     GAVLDVASCPAAEDILKTSESVEPLDPVPCPITLAWPAKDKILPPDVNGVTARERVPQ
                     AKYVVLPEVGHVPMVDDPWLCADTILVTTGAVRARWSSHDI"
     gene            complement(6703..6924)
                     /locus_tag="VGP27_25240"
     CDS             complement(6703..6924)
                     /locus_tag="VGP27_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854641.1"
                     /translation="MNADENTHPRRRRAPMVGVVLMAAGAGLADLPGATAPANADDLK
                     IVPAVPQFRDRNVTITCDNGTTTEITTKV"
     gene            complement(7045..>7230)
                     /locus_tag="VGP27_25245"
     CDS             complement(7045..>7230)
                     /locus_tag="VGP27_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7854642.1"
                     /translation="MSVSAMVVVAQMGTTIGMGLLFDTLVIRAFMTPSIAALLGRWFW
                     WPQLVRPRPVPARWSVR"
BASE COUNT         1272 a         2219 c         2423 g         1316 t
ORIGIN      
        1 agcgagggaa ttacccgcca gccggcccct gcgcgaatcc caacgaacgg ggtatcccac
       61 cgatatggat ggcactgcac ccgccagcgt gcacaccgtg ctcgccgatg ccgacggcaa
      121 cgcgtatcgg tcattgcggc agcgaccggt aacacgggcc gagcgctacg cccttggcaa
      181 aagtctgcgt aagcgggtgc cgcgcaaatc gctggccgac tggacggtgc tttctgaccg
      241 ccccgatcct gtggaactca tcaacatcag tcaccgcggg agggtcgacc ggctgatccc
      301 gatccgggtc ggccgcatgg tcgactcccc gtacggattt ctgcgcggaa ccgcggtggt
      361 gatggccgag gacgtcgcgc atctgcccgc aacgggaatc acacccgtgg tgtgcggcga
      421 ctcgcacttc ggcaacttcg gtttctacgc atctccggag cgggatctgg tgatcgacct
      481 caacgacttc gacgaggccc acccaggggg ctgggagtgg gacctgcggc ggttggccgc
      541 gagtatctgg gtcgccgggc gccacaacgg cacgtcggag gaccactgcg gcaacgcggt
      601 gcggtcctgt gtggcgtcgt atcgtcagga attgcgtcgg ctcgccgact tgccgctgtt
      661 cacaaggtct ttcgttcggc ttgatgtgga caggctggca gcagagaccg caggcccgct
      721 cagcgaacag gtcgtgcggt cggcgaagcg ggctcgtcag cgcactggcg accgggcgct
      781 gccgcgcttc acccatgagg tggatggtgc gcgcaggatt gtcgaggaac cacccctgat
      841 cacgcgactg cctccgcgtg aagcggactt actcgcacag gcactcgacg agtacctcga
      901 aactctgtcc tcgtactggc gacgtgttct cggcggctac acgttggtcg acgttgcgca
      961 caaggtcgtc ggggtgggca gtgtcggcct gcgcgcctac gtggcgctgc tcgagggttc
     1021 gtcggaatcc gacgttgttt tcctacagct caagcaggca cgccgatctg tgcttggacg
     1081 ctacgtgcac ggtgaatcgg catggcacgc gcaccagggc cagcgggtcg tggaatatca
     1141 gcaatcgctg cagacggtca gcgacccact attgggatgg accacgatgg acggccggca
     1201 gtactacgtg cgtcagttcc gaaacatgaa gggacgcata cctttagacg ccatcgatgc
     1261 ggccgcgttg actgactacg caggagtggt gggtcaacta ctcgccaagg ggcacgccag
     1321 gaccagcggc gcctcgatga tcgccgggta catgggcagc tccgaccggg ttgacaaggc
     1381 attgtgcaag ttcgcacggc gttacgccga tcagaccgaa gccgaccacg ccgcactggt
     1441 ccgcgccgta gaccgaggac tcctgcccgt cgagcgcggg gtctaggagg tatcgacgtg
     1501 caggttcgcc gggcgggtcc atccgatgtg gcggtcatcg agcacatcgt gcacgccgct
     1561 ttcggcatct atgttgcccg gatcggccga cagccggcgc cgatgactgc cgactaccgt
     1621 gccgctgtcg cgtcgtcgcg agtctgggtg atcgacgctg acggcgaggt cgccggggtc
     1681 gtcgtcaacc aggtgcacga cgaccatctg ttgctcgaca cggtcgcagt cgcccccggc
     1741 gcgcaggggc acggctatgg cgcgctgttg ctcgcccgtg ccgaggacga tgcccgcgaa
     1801 ctcgggctgg cggaggtcag gcttgttacc aaccaggcga tgaccgagaa ccaaacgttt
     1861 tacccgcgtc atggatatat cgagacgggg tgcggccgcc aggatgggta ccaccgcgtt
     1921 ttctacgcca aaaacgttgc cgcgcaatag ctggcgctac gttgccctgg ccgcgggtca
     1981 gcgagtgtta gtcgtgttcg cggaccgggc ccgcccacag cggtgggcgc gaagctccgg
     2041 gcatctggct gtggcggtaa atgatcggta tccgaacggg tcgagcacgg gaaggtgtgc
     2101 acgacaccgg ttctgcgtgg tcgcggccgt cgcggtgcgc ggcggtgcgg agccgagtgt
     2161 aagcggcgac cacgcgttgc agatcggcgt cggaggcctc ggtgagtcct ggggtcgcgc
     2221 gccgtcgggt gtcgggatgc agcgcgcgca gccggctgcg gtaggcatgg gtgatctcgg
     2281 cccgagtggc tgtaggtgtc agacccaaaa cggtgtaggg atcgtcggtt tcatgcatct
     2341 ctttcacctt ccggcttcgc cggcatagcg actctgcaac atggggccaa cccgagggtt
     2401 gccgtggtgg gtccaaaagg cggttccgtc agatattttc gcgggcgccg aaaagatgag
     2461 caggccgggg cggcatgtga agtcggcgtc cgacacaggc cgtcccggcc ggcaacaact
     2521 cggcgatgcc cagaaggact gccactagcg gtggcgctgc gcgcgcagat ctctacgaca
     2581 aatactgcgt caggcgcgga cacacaattc ctcaattttt gggcggtgcg gagtggctgc
     2641 ttgctcgtgg taatcgatca gggcatcacc tccattgttg cgcaacgatc ttccacgaaa
     2701 aggggctgag aggcgcatgt ctcagcgggg gggaacgctg ggtagctgcg actcgcaggc
     2761 aggggtacgc tcgtgccggc cactgcgtgg tgtgacggga gtgggctact ttgggccgcc
     2821 gttgcaatgc ggttgtagtg actgcaatgg gcgggcgagg ccggggcttg ggcatctcgg
     2881 cgaacggatc ggcgagcgca tcctccggca ggtcgccgcc gctgaggcgc atccattcgg
     2941 cctccacgat tgcctccgcc tcaccgatga cggcgacggc ctcggtgacg gtgctcatcc
     3001 actcaccccc ttcgcgctgc agacccggcc tcgccctgct gtcaggcgtt gatcggcttc
     3061 ttctcgctgt tgtggctgat ttcgatgcgc cgcggcttgg ccttctcggc gaccgggatt
     3121 ctcagctgga gcaccccgtc gtgatagctg gcctcgatcc gatcggcgtc gaggttgtct
     3181 cccaggaaca cttggcggct gaacactccg cgcgggcgtt ccgcggacac catctcgcga
     3241 ttgggatcgg gggctgaccg ttcggcgtgc acggtcagga cattgcgctc gacatccaga
     3301 ccaagcgaat caccattcac gccgggcagg tcgaactcga cgatgaactg atcaccttcc
     3361 ctccaggcgt ccatcggcat caccgccgga cgcgccgcag tgccccacac ctgctgagcg
     3421 aagcggtcga ggtcgcggaa cgggtcggta cgtaacaaca tccttccacc tcctaccaga
     3481 tgagagtcgg tcccacagat ctgtgtgatc tgtcataggt tttatatagc accggtgata
     3541 gcttgattca agaagaaatc ggccgggatc ggaaaaaaaa tttgatggca gatgatgcaa
     3601 cactgcgatc ggcacggggc gtgtacggca tctcggtgac cgccgagttg tcgggggtcg
     3661 atccgcagac gctgcgcctg tatgagcggc gcggactgat cacgccggcg cgaaccgacg
     3721 gcggcacccg acgctacagc gatgacgacg tggcccgcgt ggctcgcatc accgaactgg
     3781 tcgatgcggg ggtcaacctg accggcatcg gccacatact ggacctgcaa gaccgcaaca
     3841 cccgacttga agccgacaac gcccgactca gcccgaacgc cgaccaaagc gcaggccgcc
     3901 agggcagcgg tgatcgaaac gagatacgag gactccgcag cggggctcag ctgccgggcc
     3961 attctccggt ccatcgttgg aacagtcgtg caccttggcg gtcgatgacc gttttgacca
     4021 cggagaagat cgcgccctga atggcggcgg cgatcaggat ctccttcaac ggatactcgg
     4081 tttcgagcgc ttttggtggg tcgggcttgt cgccgggcgc cgcgcgttgc catacctgtc
     4141 tgaatatgac gccggccaat aagccgccca ggaccgaact gatcagacct accggtcgat
     4201 acaggatctt cgccgatgtg ctcgatttct ggtgggccat gagaactctc ctcgtttcag
     4261 gggtagccga gatgggagac ctgatacccg tttctgaggt acgtcaccgg agctaggcgg
     4321 tcgtctcgtc ggacgaaccg ggtccgcctt cgttcagctg gttgtggaaa tggtccatgg
     4381 cggtggtcag ggtgctcatc acctggtggg ctgtttcaag ctccctgtcg gtgaatcccg
     4441 cgatggcggc gcggttggcg gcgcccaggt gtgtgaagaa ggaccgggcg agctccatgc
     4501 cgtgtggttg gtagcgcaag atcaccttgc ggcgatcggc atcgtcggtg tcgcggcgaa
     4561 tgtgtccggc ggcgatcatg cgccttacca gataggtaac ccccgcattg gtcagtccca
     4621 tccgctgccg gagctctcct gaggtcaatg gagacctcga cgtttcgccg accatgatgt
     4681 gcagcagcgc gtggaactcc gtcgcgctga gtccgtgcat gtcggcgaag tatcgcccga
     4741 tccggtcggc ctgcgcgttg agggctcgca gatccgcaga gatcactgac tccagctgcg
     4801 ctcggtcacc gaacgcggcg ccagtgccgt cctgggtcat ctgcgacatt cctggattcg
     4861 tgtgattttc gcgccgcgta cttttcgaag cttaactcag tgaaataaaa tgggtatcca
     4921 ttgctcactg agagaggaga aacgtcgatg agcggtgagc gcccggacgt tctcgatcgg
     4981 ctggatgcgg cagcgatagc tgtcgcgtct ttgaacgcgt tgctggtggc ggatggatct
     5041 ctggacgacg tgctacacgg agtggcactc aatgcggtga gagccgtccc cggcgccgac
     5101 gcggtgtcga tcaccttggt tgatctacca ctgcgcactg tcgcacacac cgatgacgac
     5161 atcttggtct tggaccagga gcagtatgcc cgcaggcaag ggccttgttt gcaggccgca
     5221 gagacgcgtc ggccagtgcg ggctgcgatg ggacccggcg agcggcggtg gccggaattc
     5281 gtctcggctg cccgcgcggc cggtgtgcgc gccactctgt cgattccctt gatcatcgcc
     5341 ccggcagtgc cgggcggtga cgatgagttg gtcggttcgt tgaatgcgta cagtcgttcc
     5401 accgcggcgt tcgatgtcgt cgacgagaaa ctcatgcgtc tctacaccga tgccgcgggc
     5461 caagccgtga cggacgcgcg acgctggcaa cggctgcgcg aaacggtggg ccaactcgag
     5521 gaggcgctgg tgtcgcgtgc ggacatcgac caggccaaag gggtattgcg cacgcagcac
     5581 ggcggcaccg cagacgaggc tttcgcccgg ctggtcgagc gatctcagcg ggagaacgtc
     5641 aagatccgag acatcgcacg ccgcatcctc gagggacttc cgcggcgcaa ctgcaacgga
     5701 taaccctaaa aagaactctt ctcgagcgtg aatcaccctg cgaccgaacc ggttcgagcc
     5761 ggtgctgttt ttttcgtcgg accatggggt atcggcatcg atcagaacag atgcaatgac
     5821 cggtcgctgc atctgttcag gccgcgccgg ttcagaacgg agccggcggg gccgccccga
     5881 ccgacacacc gcgatttcga gcagttggtc cttgctcaga tgtcgtgaga cgaccaccgc
     5941 gcacgaactg ctccggtggt gacgaggatg gtgtcagcgc ataaccacgg gtcgtcgacc
     6001 atcggtacgt gaccgacctc ggggagcacc acgtacttgg cctgcgggac ccgctcgcgc
     6061 gcggtgacgc cgttgacgtc cggcggaagg atcttgtcct tcgccggcca tgccagggtg
     6121 atcgggcacg gcaccggatc cagaggctca acgctttcgg aggttttcag aatgtcctca
     6181 gccgcagggc agcttgcaac gtcgaggacg gcccccaggg cctgagcagc ggtgagccga
     6241 tcaccgtgct cagcgctatc ccgcatcgcc agccgacgca gttgaccgat gcggagtaac
     6301 gccggcgcta gcggccgaac cagccgcgcg cgacgtctga ctcggcggac catgttcgtg
     6361 gcatgagtct catcgctcgc gccgggtgtc cagaaaccgg ccggtgagaa ggcgcagacc
     6421 gtgagcgcgc ggccacggcg ggccagttcg atggccatcc acccacccat cgaattgccg
     6481 gcgatgtgga cgcgaccgag tcctcgggca tcgagaagac gttccgtctg atcggtgagg
     6541 gctgcaatgc tggccgaacc ggtgagtcgc ggcccacctc gatggccaat tgcattgggc
     6601 gcgatgatct cgtggtaatc ggtcatcagc gggacgacgt cggcccatgc ggcagcggac
     6661 atcatcacgc catgcaggag aagaagcggc tccacgacga gctcagacct tcgtagtgat
     6721 ctcagtggtg gtgccgttgt cgcatgtgat cgtcacgttg cggtcccgga attgcgggac
     6781 ggccggcact attttgaggt cgtcggcatt ggccggcgcc gttgcgccgg gcaggtcagc
     6841 gaggcccgct ccggcagcca tgagcacgac gcccaccatg ggggcccgac gtcgtcgcgg
     6901 gtgggtgttc tcatcggcgt tcatggtgtc cggcctcgaa tctcacccgg gttggtgatg
     6961 accctgagac accgttcgtt cgctcgatgt gacaggcgcg ctgtttgtgt gggctaagtc
     7021 actgtcactg atcagtgtga gaagtcagcg caccgaccac cgcgccggta caggtcgagg
     7081 acgcacgagc tgcggccacc agaaccatcg gccgaggagt gccgcaatgg agggcgtcat
     7141 gaacgcccgg atcaccaaag tgtcgaacaa cagacccatc ccgatggtcg tgcccatctg
     7201 cgcgaccacg accatggcgc tgaccgacat
//
LOCUS       DASYLX010001078         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3368998,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001078 DASYLX010000000
VERSION     DASYLX010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368998"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..792)
                     /locus_tag="VGP27_25250"
     CDS             complement(13..792)
                     /locus_tag="VGP27_25250"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV7854643.1"
                     /translation="MMLGVIGGSGFYSFFGSDARSVNLDTPYGEPSAPITVGTVGNHE
                     VAFLPRHGVKHEFSPHTVPYRANMWALRALGVRRIFGPCAVGSLTPELGPGAMVVPDQ
                     LVDRTRSRADTYFDSGGIHVEFADPYCPLLRAAVTDLPGVVDGGTMVVVQGPRFSTRA
                     ESQWFARQGFTLVNMTGYPEAVLARELEMCYAAIALVTDVDAGIAVGRGVRAVDVFAE
                     FERNLVPFKKLVHEAIDQVTKERLCTHCLPHEGVELPIDLP"
     gene            complement(789..1001)
                     /locus_tag="VGP27_25255"
     CDS             complement(789..1001)
                     /locus_tag="VGP27_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854644.1"
                     /translation="MEDRLSKSEIRKDALQETVQSVASTVGEVASIVTTAVKDVAGAI
                     GGLATDVFEIRDGVRRADKRTDDTME"
     gene            1092..>1606
                     /locus_tag="VGP27_25260"
     CDS             1092..>1606
                     /locus_tag="VGP27_25260"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEV7854645.1"
                     /translation="MASVAQWVEGARPRTLPNAIAPVIAGTGAAAWLDSAVWWRALLA
                     LVVAVAMIIGVNYANDYSDGIRGTDDVRAGPLRLVGSRLATPRSVLTAAMASFALAAA
                     AGLTLAIVSAPWLIAVGVVCIAGAWLYTGGKKPYGYLGLGEVAVFVFFGLVAVLGTQY
                     TQALRVDWVGLA"
BASE COUNT          247 a          553 c          534 g          272 t
ORIGIN      
        1 tcagcagcac cctcacggca gatcgatcgg cagctcgacg ccttcatgcg gcaggcagtg
       61 cgtgcacagc cgctccttgg tgacctggtc gatcgcctcg tgcacgagct tcttgaacgg
      121 cacgaggttg cgttcgaact cggcgaacac gtccaccgcc cgcactccac gccccacggc
      181 gatcccggcg tcgacgtcgg ttaccaaagc gattgccgca taacacattt ccaactcgcg
      241 cgcgagcacc gcctcggggt aacccgtcat gttcacgagc gtgaagccct gtcgcgcgaa
      301 ccactgactc tcggcgcgcg tcgagaaccg cggcccctgc accacgacca tggttccgcc
      361 gtcgacgaca ccgggcaggt cggtgacggc cgcccgcaac agcgggcagt aggggtcggc
      421 gaactcgaca tgaatgccgc cagagtcgaa gtaggtgtcc gcgcggctgc gggtgcggtc
      481 gaccagttga tcgggcacca ccatcgcgcc gggtcccagc tcgggcgtca ggctcccgac
      541 cgcgcacgga ccgaagatcc gccgcacgcc cagcgcacgc aacgcccaca tgttggcgcg
      601 gtacggaacc gtgtgcggcg agaactcgtg cttgacgccg tggcgcggca gaaacgccac
      661 ttcgtggttg ccgacggtgc ccaccgtgat cggcgcgctc ggctcgccgt agggggtgtc
      721 cagattgacg cttcgcgcgt ccgagccgaa gaacgagtag aagccgctgc cgccgatgac
      781 tccgagcatc actccattgt gtcgtcggtg cgtttgtctg cgcgccggac cccgtcccgg
      841 atctcgaaga cgtcggtggc cagcccgccg atcgcacccg ccacgtcctt cacggcggtg
      901 gtgacgatag aggccacctc gccaaccgtc gaagcgaccg attggacggt ctcctggagg
      961 gcgtccttgc gaatctcact cttgctgagt cggtcttcca tgcccatcag tcttcggctg
     1021 caagtgggac caaacaatgt gtccgtcgtc actccgtccg ctattcgggc ggccatgaaa
     1081 cgatggcgtc cgtggccagt gtcgcgcagt gggtcgaggg cgcccgcccg cgtaccttgc
     1141 cgaacgccat cgcacccgtg atcgcaggga ccggcgccgc ggcctggctg gacagcgcgg
     1201 tgtggtggcg ggccctgctg gcgctggtcg tcgcggtcgc aatgatcatc ggcgtgaact
     1261 acgccaacga ctactccgac ggcatccgcg gcaccgatga cgtccgcgcc ggacctctgc
     1321 ggttggtcgg atcacgcttg gccacaccgc gttcggtgct caccgccgcg atggcgagct
     1381 tcgcgctggc cgctgcggcc gggctgacgc tggccatcgt gagcgcgcca tggctgatcg
     1441 cggtcggcgt cgtgtgcatc gcgggcgcat ggctctacac cggcggcaag aaaccgtacg
     1501 gatatctggg gctgggcgag gtcgcggtgt tcgtgttctt cggtctggtc gcggttctgg
     1561 gcacgcagta cacccaggcg ctgcgggtcg actgggtcgg ccttgc
//
LOCUS       DASYLX010001079         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2052880,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001079 DASYLX010000000
VERSION     DASYLX010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052880"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..912)
                     /locus_tag="VGP27_25265"
     CDS             complement(211..912)
                     /locus_tag="VGP27_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEV7854646.1"
                     /translation="MDRTRGETTPAKEPHAAFASVGTVYYPVLAAVFTALVIISNITA
                     TKGVAFGPIITDGGFIVFPLTYVIGDVLSEVYGFKAARRVIFLGFAMNILAAVALWIT
                     IYLPAADFYANQPHFENVVHAYTQLIVAGLAGFIVGQTINAWVVVLIKERTKEKHLWA
                     RLVGSTFAGQLGDTLVFCSIAANAIGINSVGVFAVYTAQGWLYKTAVEVVMLPVTYRV
                     IAFIKRREPTYGTAV"
     gene            complement(928..1848)
                     /locus_tag="VGP27_25270"
     CDS             complement(928..1848)
                     /locus_tag="VGP27_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7854647.1"
                     /translation="MTAAVATVAACGSRKPGDVAKDGSVTVKHAFGETKVPATPKRVV
                     SAGLTEQDDLLAVGVVPIAVTNWWGDEPFGVWPWALPKLGGAQPEVLDLSNGIQIDRI
                     SALKPDLIVATNAGVDADSYNELSKIAPTIPQSGQDAFFEPWKDQAASISQAVFKFDE
                     MAALVKGVDDKFTEVGKSNPQFTGKKVLLLEGTFVDDTIHVLTPGWRADFLTGMGFVV
                     PGTGGSVITRDKAAAVLDEADILIWATESDDQKAALLADPTVAGLTATTANRNVFTGK
                     DLAGAIAFASPLSYALVADQLPPALARVLG"
BASE COUNT          350 a          627 c          631 g          342 t
ORIGIN      
        1 cgccgagcag gtcggcgtcc cgaacgacgt cggcccacgc caatacctcc gctgtgctgt
       61 cgaactggag cagcaccgcc gcatcgccgt agtcggtgac tctgccgatc accatcgcgt
      121 ccgttgtcac ggtcatctcc ctaagctacc cgccggtagg gtacgaagcc gcagctctat
      181 gagggttgcc agagctgcct taacgaagcc tcagacggca gtcccatatg tgggctcgcg
      241 gcgcttgatg aaggcgatca cccggtaggt gacgggcagc atgaccactt cgacggcggt
      301 cttgtacagc cagccctgcg ccgtgtagac ggcgaagacg ccgacactgt tgatcccgat
      361 cgcgttggcg gcgatgctgc agaagaccag ggtgtcgccg agttggcccg cgaacgtcga
      421 cccgaccagc cgcgcccaca ggtgcttctc cttggtgcgt tccttgatca gcaccaccac
      481 ccacgcgttg atggtctgac cgacgatgaa gccggcgagt cccgccacga tcagctgggt
      541 gtaggcgtga acgacgttct cgaaatgcgg ctggttggcg tagaagtcgg cggcgggtag
      601 gtagatggtg atccagagag caactgcggc aaggatattc atcgcaaagc cgaggaagat
      661 gacccggcgc gcggccttga acccgtacac ctccgacagg acgtcgccga tcacataggt
      721 cagcgggaag acgatgaagc cgccgtcggt gatgatcggg ccgaacgcga cgcctttggt
      781 cgcggtgatg ttcgagatga tcaccagcgc ggtgaacacc gcggcgagca ccggatagta
      841 gaccgttccg accgaagcaa atgcagcgtg cggctctttc gccggggtcg tttcacctcg
      901 cgtgcggtcc acccggctat caaaccgtca gccgagcacc ctggccaagg cgggtggcaa
      961 ttgatcggcg accagggcgt aggagagcgg cgaggcgaac gcgatggcgc ctgccaggtc
     1021 tttgccggtg aacacattgc ggttggcggt ggtcgcagtc agcccggcga ctgtcgggtc
     1081 ggccaacagc gcggcctttt ggtcgtcgct ttcggtcgcc cagatcagga tgtcggcctc
     1141 gtcgagcacc gcggccgctt tgtcgcgggt gatcacgctg ccgccggtgc ccggaaccac
     1201 gaatcccatt ccggtgagga agtcggcgcg ccagcccggg gtcaggacgt ggatggtgtc
     1261 gtcgacgaac gtgccctcga gcaggagcac cttcttgccc gtgaactgcg ggttgctctt
     1321 gcccacctcg gtgaacttgt cgtcgacgcc tttgacgagc gccgccattt catcgaactt
     1381 gaacaccgcc tggctgatcg aggcggcctg gtccttccac ggctcgaaga aggcgtcctg
     1441 gccggactgc gggatcgtcg gagcgatctt cgacaattcg ttgtacgagt ccgcatcgac
     1501 gcccgcattg gtggcgacga tcaggtcggg tttcagtgcc gagatcctgt cgatctggat
     1561 gccgttggag agatcgagga cctcgggttg cgccccgccg agtttgggca gcgcccacgg
     1621 ccacacaccg aacggctcgt cgccccacca attggtcacc gcgatcggta cgacgccaac
     1681 ggccagcaag tcgtcctgct ccgtgaggcc cgcgctgacc acccgcttcg gggtggcggg
     1741 gaccttcgtc tcgccgaacg catgcttgac cgtgacggaa ccgtccttgg ctacgtctcc
     1801 cggcttcctc gaaccgcatg cggcgacggt ggcgacggct gcggtcatcg ccagaaaacg
     1861 tcgcctcgac caatctttcg acaccacggg agcgtaaccc gcacgcagct aactagtgcg
     1921 aagcgcaatc agcgagcccg cgcaacacca
//
LOCUS       DASYLX010001080         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_405653,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001080 DASYLX010000000
VERSION     DASYLX010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405653"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGP27_25275"
     CDS             complement(<1..500)
                     /locus_tag="VGP27_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854648.1"
                     /translation="MIKPDNQNSEFQLGGINHVALVCSDMQKTVDFYSGVLGMPLIKS
                     LDLPGGMGQHFFFDAGSGDCVAFFWFADAADRVPGISSPAAIPGIGEITSSVSSMNHL
                     AFHVPAEKFDDYRQRLKDKGVRVGPVLNHDESEVQVSPTVHPGVYVRSFYFHDPDGIT
                     LEFACWV"
     gene            611..835
                     /locus_tag="VGP27_25280"
     CDS             611..835
                     /locus_tag="VGP27_25280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7854649.1"
                     /translation="MRVSPCEQMPAARRRRAQPATDTAARAVVARNRILATTIVDIAD
                     EAGMSTASFHSYHGSMESTVRECHEETGPT"
     gene            832..1971
                     /locus_tag="VGP27_25285"
     CDS             832..1971
                     /locus_tag="VGP27_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854650.1"
                     /translation="MTRTPSSSTPGVMREFIGLDSPTARRAGSGGHPCQGLYHRGVGR
                     KPKVAMIAVHYQIDFSEHYLADYMATRGVGFLGWNTRFRGFESSFMLDHALVDIGVGV
                     RWLREAQGVEAIVLLGNSGGGSLMAAYQAQAVDPHVTPLEGMRPAAGLTELPPADGYV
                     ASAAHPGRPDVLTAWMDASVLDENDPVSTDSDLDLFDPANGPPYSDDFVTRYRSAQVA
                     RNNAITDWAERELKRVRAAGFSDRPFTVLRTWADPRMVDPTLEPTKRPANMCYVGEPV
                     KANRSAHGIAAASTLRSWLGMWSLRHAQTRAEPHLARIDCPALVINAEQDTGVYPSDA
                     QRIHDALRSRDKTKYSVDTDHYFTTPGARSDKADTIAKWIARRWR"
     gene            1947..>2816
                     /locus_tag="VGP27_25290"
     CDS             1947..>2816
                     /locus_tag="VGP27_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854651.1"
                     /translation="MDREAVALAADSSRERSSVSVLAHFVPGAKVLEFVAPEADWLDV
                     RYCAEDDDATFYRELPEAEVIWHVLRPLSAADLEQASRCRLVHKLGAGVNTIDVEAAS
                     RLGIAVANMPGANAASVAEGAVLLMLAALRRLPELDRATRAGTGWPSDPSLGETVRDI
                     GGCTVGLVGYGNIAKRVETIVAAMGADVLHTSTADDAHPGWRPLPDLLAAADIVSLHL
                     PLTASTSNLLDASALALMKPSAVVVNTSRGPIIDEDALVDALRGGRLAAAGLDVFAVE
                     PVPADNPLLALDNV"
BASE COUNT          496 a          956 c          906 g          458 t
ORIGIN      
        1 acccagcagg cgaattcgag cgttatgccg tcggggtcgt ggaaatagaa cgaccgcacg
       61 tagacgcccg gatgcacggt cggcgagacc tgcacctcgc tctcgtcgtg gttgagcacc
      121 gggccgacgc gcacgccctt gtccttcagc cgctgccggt agtcgtcgaa cttctcggcg
      181 ggtacgtgaa acgccaggtg gttcatcgag ctgaccgagc tggttatctc gccgataccc
      241 gggatggcgg caggcgagga gatgccgggc acccgatccg cggcgtcggc gaaccagaag
      301 aacgccacac agtcaccgct gcccgcgtcg aagaagaagt gctggcccat gccgcccggc
      361 aggtcgagcg atttgatcag cggcatgccc agaacacccg agtagaagtc gacggtcttc
      421 tgcatgtccg aacacaccag cgcgacgtgg ttgatcccgc cgagttggaa ctcggaattc
      481 tgattgtcgg gcttgatcat gtcgcctccc ctcgagccga ctatgcgaat cctctgtcag
      541 gaggcagatc tgaatctaac atcaggttgg ggtgcgatca acgtccgttc agccaggagc
      601 gaggggggtc gtgagggtca gtccgtgcga gcagatgcct gccgcccggc gtcgtcgcgc
      661 gcagccggcg acggataccg cggcgcgagc tgtcgtcgcg cgcaaccgaa ttctggcaac
      721 cacgatcgtg gatatcgccg acgaagcagg tatgtcgacc gcttcgtttc acagctacca
      781 cggctcaatg gagtcgaccg tccgcgagtg ccacgaggag accggaccga catgaccagg
      841 acaccgtcct ccagcacacc cggagtgatg cgcgagttca tcggtttgga ctcccccacc
      901 gcgcggcggg ccggctcggg cggccatccc tgccagggcc tgtatcaccg cggggtcggg
      961 cgcaagccca aggtggcgat gatcgccgtc cactatcaga tcgacttctc cgagcactat
     1021 ctcgccgact acatggccac ccggggcgtc ggcttcctgg gctggaacac ccggttccgg
     1081 ggcttcgaga gcagcttcat gctcgaccac gcgcttgtcg acatcggtgt aggcgtccgc
     1141 tggttgcgcg aggcccaggg ggtggaagca attgtgctgc tgggaaactc cggcggtggc
     1201 tcgctgatgg ccgcctatca ggcccaggcc gtggatcctc acgtcacgcc tctggagggc
     1261 atgcgtccgg cagcaggtct caccgagcta cctccggccg acgggtacgt cgccagcgcg
     1321 gcacatcccg gtcgtcccga cgttctgacc gcatggatgg acgcgtcggt tctggacgag
     1381 aacgatccag tctcaaccga ttccgatctt gacctgttcg acccggcgaa cgggccgccc
     1441 tacagcgacg acttcgtcac ccgctatcgg tcagcacagg tggcccgcaa caacgcgatc
     1501 accgactggg ccgaacgcga gctcaaacgc gtgcgcgccg cgggcttctc cgaccgcccg
     1561 ttcaccgtcc tgcgtacctg ggcggacccc cggatggtcg atccgacact ggaacccacg
     1621 aagcggccgg ccaacatgtg ttacgtcggc gaaccggtca aggccaaccg atcggcgcac
     1681 ggaatcgccg ccgcgtcgac gctgcgcagc tggctcggca tgtggagcct gcgacacgcg
     1741 cagacgcgcg ccgaaccgca cctggcacgc atcgactgcc ccgccttggt gatcaacgcc
     1801 gaacaggaca ccggcgtcta tccatccgac gcccagcgca tccatgacgc actcaggagc
     1861 cgagacaaga cgaagtactc cgtcgacacc gaccattact tcaccacgcc gggagcgcgt
     1921 agcgataaag ccgatacgat cgccaagtgg atcgcgaggc ggtggcgcta gctgccgact
     1981 cgtcgcgcga gcgctcgtcg gtgagtgtcc tggcccattt cgttcccggt gccaaggttc
     2041 tcgagttcgt tgcgccagaa gcagattggc tcgacgtacg ctattgcgcc gaggacgacg
     2101 acgccacctt ctatcgcgag ctgccggaag ccgaggtgat ctggcatgtg ctgcggccac
     2161 tgtcggcggc cgatctcgag caggcgtcga ggtgcaggct ggtgcacaag ctgggcgcgg
     2221 gcgtcaacac catcgacgtc gaagcggcat cgcggctagg catagcggtg gccaacatgc
     2281 ccggcgccaa cgccgcgtcg gtcgccgaag gcgccgtcct gctgatgctt gcggcacttc
     2341 gccggctacc cgaactcgac cgggccaccc gcgcggggac cggctggccg tcggatccga
     2401 gcctcggtga aaccgtgcgc gacatcggcg gctgcaccgt cgggttggtc ggctacggca
     2461 acatcgccaa acgtgtcgag accatcgtgg ccgcaatggg cgccgacgtc ctgcacacca
     2521 gcaccgccga cgacgcccat cccggctggc ggccgctgcc tgacctgctg gctgcagccg
     2581 acatcgtgtc gctgcacctg ccgctgaccg catcgacgtc gaacctgttg gatgcttcgg
     2641 cacttgccct gatgaagccg agcgcggtcg tcgtgaacac ctctcgcgga cccatcatcg
     2701 acgaagacgc tctcgtcgac gcgttgcgag gaggtcggct ggcggcggcg ggactcgacg
     2761 tcttcgccgt cgaaccggta cccgccgaca acccgttgtt ggccctcgac aacgtc
//
LOCUS       DASYLX010001081         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_187622,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001081 DASYLX010000000
VERSION     DASYLX010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187622"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGP27_25295"
     CDS             <1..691
                     /locus_tag="VGP27_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEV7854652.1"
                     /translation="GVTVDVSDRLTLDLRRQIDRAGRWLLNYRPQPLAVGAEINRFAK
                     KVAELTPRMREWLRGDDRAIVEKEVGQFSAHGVPEELAYIVASGLYQYSLLDIIDIAD
                     IVDREPAEVADTYFALMDYLGTDGLLTAVSSLPRDDRWHSLARLAIRDDIYGSLRSLC
                     FDVLAVGEPEERGEEKIEEWQLTNGSRVQRARRTLNEIYQSDERDLATLSVAARQIRS
                     MTRTSGTGTSG"
     gene            733..1131
                     /locus_tag="VGP27_25300"
     CDS             733..1131
                     /locus_tag="VGP27_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7854653.1"
                     /translation="MASVPVRWSDIDMYQHINHATMVTLLEEARIPFLREPFEDVIDE
                     IGLLIHEVKILYKGQLRLRDSPLQVTMWSKRVRAVDFTIGYEVRPVNKPVEGRPAVIA
                     ETQLATVHIKEQRLERLSPAQLDYLRRYLR"
     gene            1128..1817
                     /locus_tag="VGP27_25305"
     CDS             1128..1817
                     /locus_tag="VGP27_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854654.1"
                     /translation="MSSPERGIWLEDATHREDLATFVERAVRLDDAAVIRLRERAGGS
                     VVAWVATGFDVLASRVVAGRVRPADLSAGGDALAKGLSAMDRGGHVDPGFPMDSAWRS
                     ALPPDTGFAHIDDVPARVVLDLAQRGVALAKEHGSEHGPPVSLLDQEVLAVSSDCGDV
                     DIPMRCVFALTAMGFLPQATAAGRAESLDPETVAADEIVRVRALPAWLRIDARFGSVY
                     RRRGDPAVVLR"
     gene            complement(1814..>3028)
                     /locus_tag="VGP27_25310"
     CDS             complement(1814..>3028)
                     /locus_tag="VGP27_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV7854655.1"
                     /translation="PGTDARQRYIFRDGTGPDGIHPPNNWVSIFGGPAWTRVTEADGN
                     LGQWYLHLFDAEQPDLNWENREVYDDLEKTLRFWLERGVDGFRIDVAHGMAKPPGLPD
                     MEIVEAEVMLADMDDDPRFNHDGVHEIHRHIRTVLNDYPHAVAIGEVWVYDNEQFARY
                     LRADELHLGFNFRLLRAEFDAADIRGAVENSLAAAAIEKATPTWTLSNHDVDRDVTRY
                     GGGDVGLARAWAMALLMLALPGAVFIYNGEELGLANVDLPDEALRDPVWERSGHTRRG
                     RDASRVPIPWEGAAPPFGFSTNSDTWLPLPKKWSSLTVEKQLKDPGSTLSFYREALRL
                     RFSRGEFDGTAIEWLDSPADTLVYRVVDGGLVCALNAGSQPLALPAGEVLLASAEVVD
                     GELPPNAAAWLV"
BASE COUNT          498 a          971 c         1054 g          505 t
ORIGIN      
        1 cggtgtgaca gtggacgtgt cggataggtt gacgctggac ctgcgtcgtc agatcgaccg
       61 cgccggccgc tggttgttga actaccggcc gcagccgctg gcggtcggcg ccgagatcaa
      121 ccggttcgcc aagaaggtgg cagagctgac accgcgcatg cgggagtggt tgcgcggcga
      181 cgaccgtgcg atcgtcgaga aggaggtcgg gcagttctcc gcgcacggcg tgccggagga
      241 gctggcctac atcgtcgcgt cgggtctgta ccagtacagc ctgctcgaca tcatcgacat
      301 cgccgacatc gtcgaccgtg agcccgcgga agtggccgac acgtacttcg cgctgatgga
      361 ctacctcggc accgacgggc tgctgaccgc ggtgtcaagc cttccgcgcg atgatcgctg
      421 gcactcgttg gcgcggttgg cgattcgcga tgatatctac gggtcgttgc ggtcgctgtg
      481 cttcgatgtc cttgccgtcg gcgagcccga ggagcgtggc gaggagaaga tcgaggagtg
      541 gcagctgacc aacggctctc gggtgcagcg ggcgcggcgc acgttgaacg agatctacca
      601 gtcggacgag cgtgatctcg cgacgctgtc cgtggcggcg cgtcagatcc gcagcatgac
      661 acgaacgagt ggaacgggga catctgggtg agcgatgagc gtcccgcgcg aggagcggac
      721 agcagggcgt ttgtggcctc agttcccgtg cgctggtcgg acatcgacat gtaccagcac
      781 atcaaccacg ccaccatggt cacccttctc gaagaggcgc gaattccgtt cctgcgtgaa
      841 ccgttcgagg atgtcatcga cgagatcggc ttgctcattc acgaggtgaa gatcctgtac
      901 aagggccaac tgcggctgag ggattcgccg ctgcaggtga cgatgtggtc gaagcgggtg
      961 cgcgccgtcg acttcacgat cggctacgag gtgcggccgg tgaacaagcc tgtggagggc
     1021 aggccggcgg tcatcgccga aactcaactg gccacagtgc acatcaaaga gcagcggctg
     1081 gaacgccttt cgcctgcgca actagattac ctgcggcgct atctgcgatg agcagtcccg
     1141 agcgcggcat ctggctagaa gacgccaccc accgcgagga cctcgcaaca ttcgtcgaac
     1201 gggcggtgcg actcgacgac gccgcggtga tccgcttgcg ggagcgggcg ggcggttcgg
     1261 tggtggcatg ggtcgcgacc ggtttcgacg tgctggcgag tcgggtggtg gcgggccggg
     1321 tgcgaccggc cgatctgagc gcaggtggcg acgcgctcgc caagggcttg agtgcgatgg
     1381 accgcggcgg tcacgtcgac cccggattcc caatggactc ggcatggcgg agcgccctgc
     1441 cacccgacac cggtttcgct catatcgacg acgtcccggc acgggtggtc ctcgacctgg
     1501 cgcagcgggg agtcgcgctc gcgaaggagc acggcagcga acacgggccg ccggtgtcgc
     1561 tgctcgacca ggaagtgctc gccgtcagtt ccgactgcgg cgacgtcgat attccgatgc
     1621 gatgtgtgtt cgccttgacc gcaatgggtt tcctaccgca ggcaacggct gccggtcggg
     1681 ccgagagttt ggacccggag acggtggccg ccgatgagat cgtgcgagtg cgtgcgctgc
     1741 ccgcgtggct gcggatcgac gcccgcttcg gctcggtgta ccgccgccgc ggcgaccccg
     1801 ccgtagtcct gcgctagacc agccaggcgg cggcgttcgg cggcagctca ccgtcgacca
     1861 cctcagcgct cgcgagcagc acctcacccg caggcagtgc aaggggttgc gacccggcgt
     1921 tcagcgcgca caccagacca ccgtcgacga ctcgatacac cagcgtgtcg gccggtgagt
     1981 ccagccattc gatcgcggtg ccgtcgaatt cgcctcggct gaaacgcaat cgcagcgctt
     2041 cgcggtagaa cgacagcgtc gaccccgggt ccttcagctg cttttcgacc gtcagcgacg
     2101 accacttctt gggcaacggc agccaggtgt cggagttggt cgagaatccg aacggcggag
     2161 cggcgccctc ccacgggatc ggcacccgcg acgcgtcgcg gccgcgtcgg gtgtgcccgg
     2221 agcgctccca caccggatcg cgcagcgcct cgtcgggaag gtccacattg gccaggccga
     2281 gttcctcgcc gttgtagatg aacaccgcac ccggtagcgc cagcatcaac agcgccatcg
     2341 cccaggcgcg agccaagccg acgtcgccgc cgccgtacct ggtgacatcg cggtcgacgt
     2401 cgtggttgga cagcgtccac gtcggcgtcg ccttctcgat cgccgcggcc gccaacgagt
     2461 tctcgacggc gccgcggatg tccgcggcgt cgaactccgc ccgcaacagc cgaaagttga
     2521 agcccaggtg caattcgtcg gcgcgcaggt accgggcgaa ctgctcgttg tcgtacaccc
     2581 acacctcacc gatggcgacg gcgtgcgggt aatcgttgag caccgtgcga atgtggcggt
     2641 ggatctcgtg cacaccgtca tggttgaacc gcgggtcgtc gtccatatcg gcgagcatca
     2701 cctcggcctc gacgatctcc atgtcgggca ggcccggcgg cttggccatg ccgtgcgcga
     2761 cgtcgatacg gaagccgtcg acaccgcgtt cgagccagaa ccgcagcgtc ttctcgaggt
     2821 cgtcgtagac ctcgcggttc tcccagttga ggtcgggctg ctcggcgtcg aacagatgca
     2881 gataccactg cccgagattg ccgtctgctt cggtgacacg ggtccacgcg ggtccgccga
     2941 agatcgacac ccagttgttc ggcgggtgga tgccgtcagg cccggtgccg tcgcggaaga
     3001 tgtagcgctg gcgggcgtcg gtgccggg
//
LOCUS       DASYLX010001082        10557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3697143,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001082 DASYLX010000000
VERSION     DASYLX010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3697143"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(448..1611)
                     /locus_tag="VGP27_25315"
     CDS             complement(448..1611)
                     /locus_tag="VGP27_25315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7854656.1"
                     /translation="MTPTVLINAGPWLTVPPVGYGGIENIIATLIPHLRRRGVRVILA
                     TVGQSTIDVDEQIHVFDEPQFAHLTEPYNDVVGVAAAHLQRIVTELRRRSDIDLVHDH
                     MEVLGPSMLAAMGTDRPTVLHTLHWDLKKHPAFYRVFGNHDCFHVNGVSAAQLQRAPE
                     KLRHHSLGHIYLATPLAHHATRRPAPAKTGHLVLVARINRRKGQHIAARLAQRYGWEL
                     VLAGPIGPFTAPEQLDAEPNADRYADVRYWRGEVAPHVDGDRVRWIGNVTGIERDDLV
                     STARATLFPVCWDEPGGTAVIESLALGTPVVGYRRGCLPELVEPGTGLLAEFGDEAAL
                     AQLITDVDQLDPANCRAAAEHRFAPGVMAAAYLKLYDQCIMGSIARPLPKAYV"
     gene            complement(1608..2660)
                     /locus_tag="VGP27_25320"
     CDS             complement(1608..2660)
                     /locus_tag="VGP27_25320"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEV7854657.1"
                     /translation="MRAGDERIPAGIDPEVWNWLSRRSTRGTSWTLQRVVKAKGARRI
                     SVVLPARNEEQTVGGIVAQVRRRLVEDVRLVDEVIVIDSRSCDGTASVAASAGATVYH
                     QDQILPDQPLMDGKGDALWKGLAASTGDIVLFVDSDLRDFTTHFIVGLLGPLLCEAGV
                     VFVKAHYLRPLVTDQVTVPGGGGRVSELVARPLINMFWPRLAGFVQPLAGEYGGYRHV
                     LEKIPFATGYGVEFAMLIDLLGLVGLDALAQVDLGTRTHRHHDIEALGRMAAQILHTA
                     SARLQREHRGNTEAPASRWLTQFRHDTASPVHHQLVVSDVAVEERPPLAEVRAVADLL
                     AAAPGTSTWPLAHQQP"
     gene            2790..3740
                     /locus_tag="VGP27_25325"
     CDS             2790..3740
                     /locus_tag="VGP27_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7854658.1"
                     /translation="MGAGGVAVRHARHLAQLKDVRIVAVTDPDMTAAQSFAEMTCAAV
                     VSDLEALLNTSPDAVYVCVPPHAHGPIEEQVLGAGIALFVEKPVALDLPTAERIADTA
                     RKAGVVTAVGHHWRYSPAVDLVRELLDGRPVRLAVGSWIDRVPPVPWWSTRALSGGQI
                     VEQAVHVLDLIRLLCGDVVEVNAYANAAPPGTPDADVDGATVAILKLQSGAVGTVAAA
                     CCVDWKHLAGLELHADGLSVTIHEDGIVARTAQGPVQRSLQPDDAKRAADSAFIDAVV
                     GNGAARSGILVDYDEALRTHQLACAIAASAVHNRPVRLYG"
     gene            3733..4827
                     /locus_tag="VGP27_25330"
     CDS             3733..4827
                     /locus_tag="VGP27_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEV7854659.1"
                     /translation="MADRRFTVVVERCGVVTLCDESLPQPVGDGQFDVATVFSGLSTG
                     TDLSWVKGTNPALHRRWDPELGLFMPGAPDVGYPIDKFGYMQVGRVTESATPAVRPGD
                     LVAMAYGHRSAYRADVLTDRFVKLPKSIEPVLGIYAAHMGPICANALLHAAADIHGTD
                     VRSLADGVRGRRIAITGAGVVGLLTALFTRLHGAESIVLAEPTPSRRHVAADLGLEVI
                     DPEDVDPAVVLKSRWRHGPGDRGADVVFQCRGQASALALALRMVRPQGTVIDLAFYPG
                     GCEEVRLGEEFHHNGLSLHCAQIGRVPRGTSHLWDRDRLSTETLALLATYGQDITRHV
                     ITDYAPLSAAPQTLIRLASSRHKGLQLVFT"
     gene            complement(5684..6115)
                     /locus_tag="VGP27_25335"
     CDS             complement(5684..6115)
                     /locus_tag="VGP27_25335"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEV7854660.1"
                     /translation="MGETGFNTFKTTEDKTNKILHDIEKAYGWPKERRNQSYAALRAV
                     LHALRDRLTVDEAAQLGAQLPMLVRGIYYTGWDPSKVPMKMHRDEFLDRIRTEFPYEI
                     EGDIEELELTVLQALRHYITDGEWEDIKSVVPKDLASALPN"
     gene            6277..6828
                     /locus_tag="VGP27_25340"
     CDS             6277..6828
                     /locus_tag="VGP27_25340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEV7854661.1"
                     /translation="MRRHEGLTPLGVVARGALAGAVGTLAMDLLWYIRYRRDGGASGF
                     ADWEFSAGLDSWDNAPAPAQFGKRVFETVFDRELAAERAPLVNNLTHWATGVGWGAAF
                     GLVAASVPTSRAPRGLVLGAVAWLQSYVVLVPAKLYKPPWEYDPVTLWKDLSAHLVYG
                     LVTDTAFRALAKPRVRAAAGSRT"
     gene            6862..6984
                     /locus_tag="VGP27_25345"
     CDS             6862..6984
                     /locus_tag="VGP27_25345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854662.1"
                     /translation="MRLRRSARYTDLVHADDRETCAYDDFRGAIADFNALISDW"
     gene            7407..8831
                     /locus_tag="VGP27_25350"
     CDS             7407..8831
                     /locus_tag="VGP27_25350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854663.1"
                     /translation="MLQRVLNRRLPVPVTPLSDGPPASPCRALRSSSMRLWVIANTVS
                     NAGAWMQMVAQNLLILQLTGSVALAGLSLSIQALPGLLLGVSGGALVDRWPRRLTAGL
                     GQVAMAAIAFTTAVFAATGMLNVPLLMVLGLMTGLVATVDGPACTLLGNDLVPRRDVP
                     SAIAVGSVAGNVGRLAGTAAGALVASVGVSVAYAANGLSFLLVAVIVPFLKLVHEPDG
                     DNDEAPALGGTRDGLSYIAKNRTLLLLVGIGALTSLLGRNYSLSMADLVTGPLGATGT
                     MYGIVTVTLAVGGIAGSIMAGRMQAPRLCTVVLLFAAASVLQVLTGISPLIVILIAVV
                     IPMAAAEAAAATSAATMLQTVPPRHLRGRVLGAWRTASTGWGLAGPPLLGLILETAGT
                     RTGLVVTGITVAAVLTLGSAAYRRRQRAAEESMASAHAGPTLVLVPTDIEASAADRVP
                     PVREHHADAPEEKWVARERELSAA"
     gene            9045..9215
                     /locus_tag="VGP27_25355"
                     /pseudo
     CDS             9045..9215
                     /locus_tag="VGP27_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(9439..9579)
                     /locus_tag="VGP27_25360"
     CDS             complement(9439..9579)
                     /locus_tag="VGP27_25360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854664.1"
                     /translation="MSASSWLGAYRRAVVDASACPIHFAITASGTPKEKRSRPDFATS
                     HD"
     gene            complement(10366..>10557)
                     /locus_tag="VGP27_25365"
     CDS             complement(10366..>10557)
                     /locus_tag="VGP27_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854665.1"
                     /translation="IAWGKLLGTLTASVSQYLYDPGNVLWLVIYIEILILDLVYVILL
                     SKAPLYQRDEVEASALTQR"
BASE COUNT         1726 a         3333 c         3463 g         2035 t
ORIGIN      
        1 ggtgcgcacc acaactcgcc gtggcggcgc acccttgaca ggcggagcgg tcatgtctgc
       61 acagccttcg ctgtttcggg ccctatcgct taggcctggc ggcgtgaatg ccgtggatcg
      121 gcttcgtcat cccgggacga cgaaatgacc gacgacggct ggcgccgcct atcgtggccc
      181 gagtgtccac cgacgaggtg cgccagctgc gcgcacgcct ggacggtctg acgatccgcg
      241 acgccgcccg gctcggccgc cggttaaggg cctgcgctcc gctgacccgg acaaggtcgg
      301 ccggatcgcc gagccgttcg ccgccgcgga ggctctggtc gccacccgtg aagcgctact
      361 tgcgccgcgt tacacgatgt ctcgctcgtg ttcggttcgc cgagcgagtc cgtcgtcgtc
      421 gagatgctca ggtggtgccg ccgatcctca tacgtaagcc ttcgggagtg ggcgcgctat
      481 cgagcccata atgcattggt cgtagagttt gagataggcc gcggccatga ctcctggagc
      541 gaaccgatgt tctgccgctg cgcggcaatt ggcgggatca agctgatcga catcggttat
      601 cagctgtgcc aatgcggctt catcgccgaa ctcggcgagt aatccggtgc cgggttcgac
      661 gagttcgggc agacagccac ggcggtaacc gaccacaggt gtaccaaggg ccagtgattc
      721 gatgactgcg gtaccgcccg gttcatccca gcacaccgga aacagcgtcg cccgcgcggt
      781 cgagacaagg tcgtcacgct caatgccggt cacattcccg atccaccgca cccggtcacc
      841 gtcgacgtgc ggggcgacct ctccgcgcca ataacgcaca tcggcatatc gatcagcatt
      901 cggctcggcg tctagctgct ccggcgcggt gaagggtccg atgggtccgg ccagcaccag
      961 ctcccatcca taccgctgtg cgaggcgcgc ggcgatgtgt tgcccctttc tacggttgat
     1021 tcgcgcgacg aggaccagat ggccggtctt ggccggggca ggccgacgcg tggcgtggtg
     1081 cgccagcggg gtggccaggt agatgtgtcc gagactgtgg tgacgaagct tttcgggcgc
     1141 gcgctgtagt tgcgcggcgg agacaccgtt gacgtgaaag caatcgtggt taccgaatac
     1201 gcggtagaag gcggggtgct tcttcagatc ccagtgcaag gtgtgcagca ctgtcggccg
     1261 atcggtgccc attgccgcga gcatgctggg gcccaagact tccatatggt cgtgaaccag
     1321 gtcgatgtcc gaacggcgtc gcaattcggt gacgatgcgc tgcaagtgcg ccgcagcgac
     1381 accgacgacg tcgttgtagg gctcggtgag gtgggcgaac tgcggctcgt cgaacacgtg
     1441 tatctgctcg tcgacgtcga tggtgctctg gccgacggtg gcaaggatga cacggacgcc
     1501 gcgtcgacgc aggtgcggaa tcagcgttgc gatgatgttc tcgattccgc cgtagccgac
     1561 cggcggaacc gtcagccacg gcccggcgtt gatcaatacc gtcggagtca tggctgctgg
     1621 tgcgctagcg gccaggtgga ggtgccgggc gcggcggcga gcaagtcggc aacggcgcgg
     1681 acctcagcca gcggcggtct ctcctccaca gcgacgtccg aaaccaccaa ctggtggtgt
     1741 acgggtgacg ccgtgtcgtg ccgaaattgg gtcagccacc tcgatgccgg cgcttcggtg
     1801 ttaccgcggt gctcgcgctg gagtcgggcg cttgcggtgt gcaagatctg ggccgccatg
     1861 cgccccaacg cttcaatgtc gtggtggcga tgggtgcgcg tacccagatc aacctgagcg
     1921 agcgcgtcta gtccgacaag accgagcaga tcaatcagca tggcgaattc cacgccatag
     1981 ccggtggcga acggaatctt ttccagtacg tggcgatagc ccccgtactc gcctgccagg
     2041 ggctgtacga agccggccaa tcgcggccag aacatgttta tcaggggccg ggccaccagt
     2101 tcgctgaccc gaccgccgcc gccggggacg gttacctgat cggtcaccag cgggcgcagg
     2161 tagtgcgcct tgacgaacac cacgcccgct tcacacagca gtgggccgag cagtcccacg
     2221 atgaaatgcg tggtgaagtc gcgtaggtcc gaatcgacga agagcactat gtcgccggtg
     2281 ctggctgcca gtcccttcca caacgcatcg cccttgccgt ccattagtgg ttggtcaggc
     2341 agtatctggt cctggtgata aacggtcgcc ccggctgacg ccgccaccga cgctgtcccg
     2401 tcacacgatc gggagtcgat gacgatgacc tcgtcgacca gcctgacgtc ctcgacgagc
     2461 cggcgccgca cctgcgcgac gatgcccccg acggtctgct cctcgttacg ggcgggcagc
     2521 acgacgctga tccgcctggc gcccttggcc ttcacgaccc gctgcagagt ccacgacgtc
     2581 ccgcgagtgc tgcgccgtga cagccagttc cacacctcgg ggtcgattcc ggccggaata
     2641 cgttcgtcgc cagcgcgcat gacatccgaa atacccctta cttcggtgtc aaatccaccc
     2701 caaagggtgg cgtattggcg gcccccgggg gtacccgtag agcgccgccg accgggagat
     2761 gaactttgga ttgctgcaag gttgcgtttg tgggagccgg tggtgttgcc gtgcgccacg
     2821 cgcgtcatct cgcccagctg aaggatgtcc ggatcgtcgc cgtgacagac ccggatatga
     2881 cggctgcaca atcatttgcc gagatgacct gcgctgccgt ggtttcggac ctggaggcac
     2941 tcctgaacac atcgccggat gcggtgtatg tctgtgttcc gccacatgcc catggcccca
     3001 tcgaggaaca ggtcctcggt gcaggcatcg cgttgtttgt cgagaagccg gtggcgctgg
     3061 acctgccgac cgccgagcgc atcgcggaca cggcgcgcaa ggccggcgtc gttactgcgg
     3121 tcgggcatca ctggcgctat tcgccggccg tcgatctcgt ccgggagcta ctcgacggcc
     3181 gacccgttcg gctcgcggtg gggtcatgga tcgaccgcgt gcccccggtg ccgtggtgga
     3241 gtacgcgggc gctgtcgggc ggtcagatcg tcgagcaggc ggtgcatgta ctggatctga
     3301 ttcgcctgct gtgcggcgac gtcgtcgaag tcaacgcgta cgccaacgcc gcgccgccgg
     3361 gcactccgga cgccgacgtc gacggagcca ccgtcgcgat cctgaagttg caatccggag
     3421 cggtgggaac tgtcgccgcc gcatgctgcg tggattggaa gcatctggcg ggtcttgaac
     3481 tccatgccga cggcctgtcg gtgacgattc atgaagacgg aatcgtcgcg cggacggcac
     3541 aaggacccgt gcagcgcagc ctgcaacccg atgatgccaa gcgagccgcg gatagcgcgt
     3601 tcatcgacgc tgtggtgggc aacggcgcgg cccgcagcgg cattctggtg gactacgacg
     3661 aggcattgcg gacccaccaa ttagcttgcg ccatagcggc ttcggctgtg cataaccgcc
     3721 ccgtgaggct gtatggctga ccgcagattc accgtcgtgg tcgagcgctg cggcgtcgtc
     3781 acgctgtgcg atgaatcgtt accgcagcct gtgggcgatg gccaattcga cgtagcgaca
     3841 gtgttcagtg ggttgtcgac aggcacagac ctcagctggg tgaagggcac caaccccgcc
     3901 ctgcaccgca ggtgggatcc agagttgggt ctgttcatgc caggcgcccc agacgtgggt
     3961 tatccgatcg acaagttcgg ctacatgcag gtcggccggg tcacggagag tgcgacgcct
     4021 gccgtgcggc caggtgacct cgtcgctatg gcctacggcc accgcagcgc ttaccgcgcc
     4081 gacgtgctta ccgaccgttt cgtcaagcta ccgaaatcaa tcgagccggt gctgggcatc
     4141 tacgccgcac acatgggacc gatctgcgca aacgcactgt tgcacgctgc cgctgacatc
     4201 catggcaccg atgtacgcag tctcgccgat ggcgtgcgcg gtcggcggat cgcgatcacc
     4261 ggtgccggtg tcgtgggtct gttgaccgcg ttgttcaccc gcctgcacgg tgcggaatca
     4321 atagtgcttg ccgagcccac gccgtcacgc cgccacgtcg cggcagacct cggcctggag
     4381 gttatcgatc ctgaggatgt cgacccggcc gtcgtgctga aaagtcgatg gcgtcacggg
     4441 cccggggacc gtggagccga tgtcgtgttc caatgtcgcg gtcaggccag cgcattggct
     4501 ctcgcccttc gtatggtgcg cccccaaggc acggtcatcg acttggcgtt ctacccgggg
     4561 ggatgcgagg aagtccgact gggggaggag tttcaccaca acggactgag cctgcactgc
     4621 gctcagatcg gacgggtgcc gcgcggaacc agccatctgt gggaccgcga tcgcctcagc
     4681 accgagacat tggcgcttct agccacatat ggtcaggaca tcaccaggca tgtcatcacc
     4741 gactacgcgc ccctatccgc cgcaccgcag accctgattc gactggccag tagccggcac
     4801 aaagggcttc aacttgtgtt cacctgaggc ggatagcccc ttgatgcgtc gatgccgcca
     4861 cgacggcggg cggtgatcat cgtggcccgc ccgcccgtca gctgcacccg caccaccatc
     4921 aagccgttgt tcttggccgc ggctattggc agcggtcggt gcggctggaa tgcgcggtgc
     4981 agcgttgcgc cgggggtcgt gcgaccgtgg ccaacaattc atctcaccat cgggccggag
     5041 ccatcgccat cgcgccgtcg gctggggtgc ccccggcaag attcgaaact gccgggttcg
     5101 ggcgtatcaa cgcaggtcag cacgctatga ccgcactagc agcggattgc tgtcggatgc
     5161 ctcagggcgt ggtgtttgaa tgcgttgttg cgcaacaggg ttcagtttcg accaggtggc
     5221 aatgctcgct gcggtcggtg cagtcttgca tgcccagcgg tgcccatatt gttgatgcgc
     5281 gccgcacgaa tcacggcctc atcgagcgtc atcaggccgt caacttccgg tgagagaccg
     5341 aacgggcttg gcgtgtcggc catcggatcg aatagcgtcg tgcgcccgtc tgcgggccaa
     5401 tcgtcatcgt ctggcgtgat cggatgggcg aagctctgtt gatcgtctgt gtcgcttgca
     5461 gggccgtctt cgagccgagg catgttgtcc aagagtgttc gtacggcttg gccgtgattt
     5521 caaccgccgt cttgggattc aaacattcga gtcggtcgcc attttgagta gttcgcgcgc
     5581 catgcggtcg gcagcttctt ctagccgttg tttggctttg cgtctcacct gcggtgcgcg
     5641 cccgccgtgc tgcgggcaca ccgtcgtggt catccatccc gacttaattc ggcagcgcgc
     5701 tggccagatc cttcggcacc acggacttga tgtcctccca ttcgccgtcg gtgatgtagt
     5761 gcctcagcgc ctgcaatacc gtcagttcca gttcctctat gtcgccttcg atctcgtacg
     5821 gaaactccgt gcgtatgcga tccaggaact cgtctctgtg catcttcatg ggcaccttgc
     5881 tggggtccca gccggtgtag tagatgccac ggacgagcat cggcagctgc gctcccaatt
     5941 gcgcggcctc atccacagtc agccgatcgc gcaatgcgtg caagactgcc cgcagggcgg
     6001 catacgactg gttgcgccgc tccttgggcc agccgtatgc cttttctata tcgtgaagga
     6061 ttttgttggt cttgtcttcg gtcgtcttga atgtgttgaa tcctgtttca cccatgggtg
     6121 aggccctccg tttcaatcgc cgactctgtc tgtcggtacc ggtcacccat gctgcgccca
     6181 acaagacgtg agcgattggg caccgcagcg ccaatcgtgg cggcggcctc cgtcctgaga
     6241 ggctcatctg cgcagaccgg gcctacgctg gtgagagtga gacggcacga gggcttgaca
     6301 cctctgggag tggttgctcg tggtgcgctc gcgggtgcgg taggcacgct cgcgatggac
     6361 cttctctggt acatccggta ccgacgcgac ggtggggcca gcgggttcgc cgactgggag
     6421 ttctccgccg gcctggacag ctgggacaac gctccggccc cggcgcagtt cggtaagcgc
     6481 gtgttcgaaa cggtgtttga ccgagagctg gctgccgaga gggcccccct ggtcaacaac
     6541 ctcacgcatt gggcgaccgg tgtgggctgg ggcgcggcgt tcggcctggt cgctgcctcg
     6601 gtgccaacta gccgcgcccc gcgcggtctg gttcttgggg ccgttgcgtg gctgcagtcc
     6661 tacgtcgtgc tggtgcctgc gaagctgtac aagccgccgt gggagtacga cccggtgact
     6721 ctctggaagg acctgagcgc ccatcttgtc tacggcctgg ttaccgacac ggcgttccgt
     6781 gcactggcca aaccgcgtgt gagggcggcg gcgggcagcc gcacctaggg tcgcgacggg
     6841 ccgtgcttac tccatcccag catgcggtta aggcgctcgg cgcggtacac cgatctggtg
     6901 cacgccgacg acagagagac atgcgcctac gatgacttcc gaggggccat cgccgacttc
     6961 aacgcgctga tctcggactg gtagcgcaag ggcggcgcgc ccaacgcccg cgatggcgcc
     7021 tcggtgcagg cccgcctcga cgggtacacc aacgagtggt gccgatcgtc ggcagatccc
     7081 gcggctggcc gcctactcca gcaagctgga ggttgcgctc agcttggtga aagccgctga
     7141 cacgatttgg ctgacccagc cgatcgccgt atcgcacggt gcctccgtca gcagcccagc
     7201 gtggatggcg taccacttcg tcgagtgaaa tcacggccct gcagcgctcg gctccggcga
     7261 ggcggggaat cctgcggaga agtgttctgt tgcacaagct atcggtgtga ctgggtaggc
     7321 tcctgagtaa gcgctttgcg aggcccgtga gtcaacaatc caggcccggc ttgccttgta
     7381 atcgactttt cacgagtgag ttcttgatgc ttcagcgcgt ccttaatcga cgccttcctg
     7441 tccccgtaac cccgctttcc gatggcccac ccgccagccc gtgccgcgcc ttgcgcagtt
     7501 ccagcatgcg gctgtgggtg atcgccaaca ccgtgagcaa cgccggtgcc tggatgcaga
     7561 tggtcgcgca gaacctgctg atcctgcagc tgaccgggtc tgtcgcgcta gccggcctct
     7621 ccttgtcgat ccaggctctg ccgggcctgc tgctcggcgt ttccgggggc gcgctggtgg
     7681 atcgctggcc gcgccgtctg actgctgggc tgggccaggt cgcgatggcg gcgattgcct
     7741 tcaccaccgc ggttttcgcc gccaccggca tgttgaatgt tccactgctg atggtgctcg
     7801 gcctgatgac cggcctcgtc gcgaccgtcg atggtcccgc ctgcaccctg ctcggcaacg
     7861 acctcgtgcc acgtcgcgac gtaccatcgg cgatcgccgt cggttccgtc gccggcaacg
     7921 tcggccggct ggccggcacc gccgcgggtg cgctcgtcgc ctcagtcggc gtatcggtcg
     7981 cctacgcggc caacgggctg tcgtttctcc tcgtcgctgt gatcgttccg ttcctcaagc
     8041 tggtccacga acctgacggc gacaacgacg aggctcccgc gctgggtggt acgcgcgacg
     8101 ggttgtcata catagcgaag aaccgcacgc tgctccttct ggtcggtatc ggagcgctga
     8161 ccagtttgct cggccgcaac tactcgttga gcatggctga cctggtgacg ggaccgctcg
     8221 gtgccacagg caccatgtac ggcatcgtga ccgtcacgct tgccgtcggc gggatcgccg
     8281 gctcgatcat ggccggccgg atgcaggcac cgcggctatg caccgtcgta cttctgttcg
     8341 ctgccgcctc agtcctgcag gtgctgactg ggatctcgcc tctaatcgtg attctgatcg
     8401 ccgtggtgat ccccatggcg gccgccgaag cggcggcggc gacaagcgcc gcgaccatgc
     8461 tccagaccgt gcccccgcgg caccttcgcg gtcgcgtgtt gggtgcctgg cgaacggcca
     8521 gcacgggctg gggtctagcg gggccgccgc tactcgggct gatcctcgag accgccggca
     8581 ctcgtaccgg actggtcgtc accggtatca ccgttgcggc cgtgctcacc ctcggcagtg
     8641 cggcgtacag gcgtcgtcag cgcgcggcgg aagagtcgat ggcgtcggct cacgccgggc
     8701 cgaccctggt gctggttccg actgatatcg aggcatccgc tgccgaccgt gtgccgccgg
     8761 tccgggagca tcacgccgat gcgccagaag agaagtgggt cgcccgcgag cgggaattga
     8821 gtgcggcctg aacccgctcc tacggtcatg agctgtccaa tttgattgcg cacactcgca
     8881 gtcagcggca ggaatcgcga agtggcgcgg gcgctgggca actgcacggt cgaacgcgcc
     8941 agtgttgctg agagtccagt cgcgtcggtg ccatggacgc ggagcagggc aatgcgagcg
     9001 tcgctctccg agtcgtcgag tgccccgact tcagcaccgc tgccctgttt cgtaaaccgc
     9061 gctccccgtt gggattaggc gccttcgtgc tggttgagct ggtctacctg ctcgcctcga
     9121 tgtcgttcgt ggtgctgttt gtcggcgacg gtcctttgcc tgcctggatg ctcgcattgg
     9181 cggtgggcgc acccacggtg atcgcgggtc gcgcgcaaga gtcggaaagt ctgttcggcc
     9241 acggctttgg cgtgatggaa atcgcctgcg ctgacgcaac ccaaccggtg cgcagtgcat
     9301 ccgaaccgat tgactcaaaa gccactgatg ccgacccttg ccactttcga tgttggcggg
     9361 atcggcttgc cgccgtcttg ggatagagcg gcgtagcctt tggcgggttg gcaagacgcg
     9421 cccgaatttc ttccggcgtc agtcgtggct tgtcgcgaag tcgggccgcg aacgcttctc
     9481 tttgggtgtg ccgctcgcgg tgatcgcgaa gtggatcggg cacgccgacg cgtcgaccac
     9541 cgcacgcctg tacgcaccca gccaggatga cgcgctcaag gatgctgcgg cgaccttagg
     9601 aacagttgtg acatcggctg tgacaacggg acgccctacg ctgacagtcg ttggcgaata
     9661 acagagcatc gacgcacgtc aagcgatgtt ttcaagggtg cccccggcag gattcgaacc
     9721 tgtcggcgtg atttcagcaa atatgctgct agaccagcat taaccttgtc caccaacagg
     9781 tttcgatctt tcgcgcttcg ctctgttcgc acaggctgca acggtctaga ctgatttgga
     9841 agggtagctc aggggtagaa gccagcaaca ctactgctca gcgccctagg cccgcaggag
     9901 tggtaacact caaaatggac agaaaacagt ttgctatttc gcggtgcatc gacggcatgg
     9961 acgggaaagt gattgaagaa tgaccgaacg aaaccgcgca cactacggat acagctcgtt
    10021 ccagaaagat gacgggcgtt cgctgccgat ttcgatggtg gcacgaagac tgccgatgtc
    10081 gcggatgacg caggtttggc caccggcctc ctgatcgcag gcgcggttct cgcactggca
    10141 gcggtcattc ggggtggggg ctacgcggtg cgggccgcgg gtgtggtgcg ttcactcagg
    10201 atcacgtctg agcaactgag gtattcgcaa accccgttca acgaagtggg ccggacggct
    10261 gccgtgcttc agcacaccgg gccatcgaag ccactcaaag tgattactgg cggcgtatgt
    10321 gccttgggtt gggtcgcagc catggctgct cccacggcga tatgctcagc gctgcgtcag
    10381 ggctgaggct tccacctcat cgcgttgata caggggagct ttcgacagca ggatcacgta
    10441 aaccagatcc aggatcagta tctcgatgta gatcaccaac cacaggacat ttcccgggtc
    10501 gtaaaggtac tgcgagaccg aggcggtcaa ggtgccaagg agcttgcccc acgcgat
//
LOCUS       DASYLX010001083         5491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2490711,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001083 DASYLX010000000
VERSION     DASYLX010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490711"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGP27_25370"
     CDS             <1..256
                     /locus_tag="VGP27_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854666.1"
                     /translation="LAIHFGDEETGAEYHPPSKWEQVTADPDHLAGLNTPYVDEDDED
                     YDDGEDYEDEDYEDEDYEDEDYEDEDYEDEVDEEPEPEDE"
     gene            359..817
                     /locus_tag="VGP27_25375"
     CDS             359..817
                     /locus_tag="VGP27_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854667.1"
                     /translation="MRTTFEFDFVTTASPDEVVELMTDFSPERARRWPASSEAAFEVY
                     RVGDTDAEIREGQDFPKLWARWRYDWSAPDSVTLTVVEAEALNPGGFMTLTATPRTEG
                     GSAVHAVWDQSSKNLNGLVAMVTMRVIGSRFLSSYYRRVYDNYSPPTRNL"
     gene            complement(801..1682)
                     /locus_tag="VGP27_25380"
     CDS             complement(801..1682)
                     /locus_tag="VGP27_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7854668.1"
                     /translation="MTMPVMEPALDAPTLQAWAKAIDDGPYSSLCWGERIAFDNPEAI
                     TLLGALAAWTDRVRLVTTVVVPQLHDPVMLAKALATGDMLSGGRLTVGLGIGGRREDY
                     EAVGADPATQTMRGMAQLVATMRRVWAGEKVTDSVLPVGPAPVQEAGPRLLVGTTGPK
                     TVQSAAAWADGLAGTTLDLDTAKQNQLYDVARTAWAWAGKPKPHLATSFWFAIGDADS
                     ARAQVHRHLRRYMNWIPIEYVDAMAPSTGWAGTEDQLASVLQQFDDIGTDEVHLIPTS
                     SDVEQVRRVADVVKDFG"
     gene            complement(1738..2907)
                     /locus_tag="VGP27_25385"
     CDS             complement(1738..2907)
                     /locus_tag="VGP27_25385"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7854669.1"
                     /translation="MGAVTRVLAVVASSVMAVCVAVCPVHADPDHDLVSRLDAAIEAR
                     IAQMGIPGAIVALSIPGEIEYAKAFGVGDTATGEPMLVNDFTRIGSVTKTFTGTAILQ
                     LVDQGKISLSDPISRYVDGVPFGDVITLDLLGRMRSGLPDYTQTDEFLNRVYTELPTG
                     PDAFPTTPQELIEAAFRQPMEFPPGTEYRYCNTNTVLLGMVVEKVAGLPLGEYFQQNI
                     FGPLDLQRTSYPPNGLLPVPYPHGYSKAPDGDIVDATLWNPSWGDAAGKIVSDLADMT
                     VWAVALGKGTLLRPDTQAQRISNGTAATPGVDYDFALFNAHGWLGHNGDIPGYTTVVV
                     YLPERNASLVVFTNSDVPEQNSAGQIAYDVTSIGTPDHLYEVGPKPPELMREPGD"
     gene            2977..3390
                     /locus_tag="VGP27_25390"
     CDS             2977..3390
                     /locus_tag="VGP27_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854670.1"
                     /translation="MTINTSAVQHVRLTVTDIARSRTFYDNVFGWAVALQVAADADAE
                     TREKLWFLYDGVIYNTGDMLVGLRPVAQDRFDENRVGLDHLAFRVSTKADLDAAAAHL
                     DDLGIAHEPVKDIGTAHILEFRDPDNIALELIALE"
     gene            complement(3423..4445)
                     /locus_tag="VGP27_25395"
     CDS             complement(3423..4445)
                     /locus_tag="VGP27_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV7854671.1"
                     /translation="MPNGFDVRDLAVPVIVAPMAGGPSTPALAAAGTNAGGLGFVAAG
                     YLSADALAERIAAARNLTTGPLGVNLFVPQPSAATPEAVETYAAALAPDVERYGAELG
                     DPSYNDDAWAAKLDVLLELRPEVASFTFGLPSVEECRRLRDAGIATVGTVTSVYEADA
                     AVDCGVDAIAVQGPSAGGHRGTFDPVADPNPQPLSEMLVAVLARVNVPVIAAGGLVTA
                     DDVKCVIEEGAVAAQLGTAFLLADESGSSPVHRAALQDPRFTETVVTKSFSGRYARGL
                     RNRFVDDHEAQAPFGYPEVHYLTGPLRAASVRAGDPDGTNVWAGTGFQSIRTGSVADI
                     MGDLAP"
     gene            complement(4572..5105)
                     /locus_tag="VGP27_25400"
     CDS             complement(4572..5105)
                     /locus_tag="VGP27_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7854672.1"
                     /translation="MANVKILTGARVGLRAPRLQDADELFASVASDPEVTRYMSWTPH
                     LDVDETRRVITELFNVGAERTWLIELRATGEIIGTCGFRTVQAHSRDFGYVLGRRWWG
                     QRLMPEVVQLLLAEMESDPSVYRVSAICHVDNTRSARVLERAGLSFEGKLVRHTVFPN
                     ISTTAPQDVLQFAKSVR"
     gene            5157..>5491
                     /locus_tag="VGP27_25405"
     CDS             5157..>5491
                     /locus_tag="VGP27_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854673.1"
                     /translation="MAITFNHTIVFSHDKKQSATFLTDLFDLPCPAPVGRFMTVTVSN
                     GVTLDYMDLPEGENVRPQHYAFLVSEDEFDAIYGKIRARGLEHWADAMSSRQGTINTD
                     DGGKGVYFR"
BASE COUNT          942 a         1789 c         1806 g          954 t
ORIGIN      
        1 gctcgccatc cacttcggtg acgaagagac gggggccgag taccacccgc cctcgaaatg
       61 ggagcaggtg accgcggacc ccgatcacct ggcggggctg aacaccccgt acgtcgacga
      121 agacgacgag gactacgacg atggcgagga ttacgaagac gaggattacg aggacgagga
      181 ttacgaggac gaggattacg aggacgagga ttacgaggac gaggtcgacg aggaacccga
      241 acccgaggac gagtagtcgg ctcaggctca agcgcggatt cactccgcgg tgagggcgac
      301 cgctactcgc acttctgtgc tgtattcgca gagtcaaagc cgctgatacg ttcgggcagt
      361 gcgtaccacg ttcgagttcg acttcgtgac gaccgcgagc cccgacgagg tggtcgagtt
      421 gatgaccgac ttctcacccg agcgagcgcg gcgctggccc gcatcctcgg aggcggcatt
      481 cgaggtctac cgcgtcggcg acaccgacgc ggagatccgt gagggccagg atttcccgaa
      541 gctgtgggcg cgatggcgtt acgactggtc cgctcccgac tcggtcacgc tgacggtggt
      601 ggaggccgag gcgctgaacc cgggtggctt catgacgttg acggcgacgc ctcggaccga
      661 gggcggtagc gcagtgcacg cggtgtggga tcagtcgtcg aagaacctga acgggttggt
      721 cgcgatggtg acgatgcgcg tgatcggctc ccggtttctg tcgtcgtact accggagggt
      781 ctatgacaac tacagcccgc ctacccgaaa tctttgacga cgtcggctac ccgccgcacc
      841 tgttccacgt cggagctggt cgggatcagg tgaacctcgt cggtgccgat gtcgtcgaat
      901 tgttgtagca cacttgccaa ttggtcctcg gtgcctgccc atccggtgct gggcgccatt
      961 gcatcgacgt actcgatcgg gatccagttc atgtagcggc gcagatgccg gtgcacctgt
     1021 gcacgcgctg agtcagcgtc gccgatggcg aaccagaacg aggtggccag atgcggtttg
     1081 ggcttcccag cccatgccca cgctgttctt gcgacgtcgt agagctggtt ctgcttggcg
     1141 gtgtcgagat caagtgtggt gcccgccaga ccgtcggccc atgctgcggc ggactgcacc
     1201 gtcttgggac ccgtggttcc gaccagcagt cgcggaccgg cctcctgcac cggcgcggga
     1261 ccgaccggaa gcacggaatc ggtcaccttc tcgccggccc agactcgtct catcgtcgcg
     1321 accagttgag ccatccccct catcgtctgc gtcgccgggt cggcgccgac ggcttcgtaa
     1381 tcctcgcgcc ggccgccgat cccgaggccg acggtgagcc tgccgccact gagcatgtcc
     1441 ccggtggcga gcgccttggc caacatcacg gggtcgtgca actgcggcac caccaccgtc
     1501 gtcaccagcc gcacccgatc ggtccacgcc gccagcgcgc ccagcagcgt gatggcctcc
     1561 gggttgtcga atgcgatccg ctcaccccag cacagcgacg agtacggtcc gtcgtcgatc
     1621 gccttggccc aagcctgaag ggtcggcgcg tcgagggccg gctccatcac cggcatcgtc
     1681 atacctacct gcacgacggc gattctggca cgccgtcgta gcggcgcccg ctggcggtca
     1741 atcgccgggc tctcgcatca attccggcgg tttggggccc acctcgtaga gatggtcggg
     1801 cgtcccgatg gatgtcacgt cataggcgat ctgccccgcc gaattctgtt cggggacatc
     1861 cgagttggtg aaaaccacca gcgaggcgtt gcgttcgggc aggtacacca ccaccgtcgt
     1921 atagccagga atatcgccgt tgtgtccgag ccaaccgtgc gcgttgaaca gcgcgaagtc
     1981 gtagtcgacc cccggtgtgg cggcggtgcc gttgctgatc cgttgtgcct gggtgtcggg
     2041 gcgcaacagc gtgcccttgc cgagggccac cgcccaaact gtcatgtccg caaggtcgga
     2101 cacgattttg cccgcggcgt cgccccacga cggattccac agggtggcgt cgacgatgtc
     2161 gccgtcgggc gccttgctgt agccgtgcgg gtacgggaca ggcaggagtc cattcggcgg
     2221 atagctggtg cgctgcaagt cgagtggacc aaagatgttc tgctggaagt actctccgag
     2281 cggaagtccg gcgacctttt ccaccaccat gccgagcagg acggtattgg tgttgcagta
     2341 ccggtattcg gttcccggcg ggaactccat cggctgccgg aaggccgctt cgatcagttc
     2401 ttgtggtgtg gtggggaatg cgtcgggacc agtgggcagc tcggtgtaaa cccggttgag
     2461 gaactcgtcc gtctgggtgt aatcgggtag cccgctacgc atccggccga gcaggtccag
     2521 cgtgatgacg tcgccgaacg ggacaccgtc gacataacgg gagatcggat cgctcaggct
     2581 gatcttcccc tgatcgacga gctggaggat cgcggttccg gtgaacgtct tggtgacgct
     2641 gccgatgcgg gtgaaatcgt tgacgagcat cggctccccg gtggcggtgt cgccgacgcc
     2701 gaacgccttg gcgtattcga tctcgcccgg gatcgagagg gcgacgatcg cgccggggat
     2761 gcccatctgg gcgatgcggg cctcgatcgc agcatcgagc cgcgagacga ggtcatggtc
     2821 ggggtccgcg tggacaggac agacggcgac gcagacagcc atcacagatg acgcgacgac
     2881 ggccaacacc ctcgtgactg cgcccataag ccggcctctc ggtctcgggt ccccgcccgg
     2941 accgacccta tcgcgtgtgc tggcagcata agggccatga cgatcaacac atccgccgtc
     3001 cagcacgtca ggctgacggt caccgacatc gcacggtcgc ggacgtttta cgacaacgtc
     3061 ttcggctggg cggtcgcgct gcaggtggcc gccgacgcgg atgccgagac ccgggagaag
     3121 ctgtggttcc tctacgacgg cgtcatctac aacaccggtg acatgctggt cggcctgcgg
     3181 ccggtggcgc aggatcggtt cgacgaaaac cgcgtcggcc tcgaccattt ggcctttcgg
     3241 gtgagcacga aggctgatct ggatgcggcc gccgcccatc ttgacgatct cggcatcgca
     3301 cacgagcccg tgaaggacat tggtacagcg catatcctcg agttccgcga tccggacaac
     3361 atcgcactgg aactcatcgc cctcgaatag agcgggtacg cgccgagcac ggccgaacgt
     3421 gcctagggcg ccaggtcgcc catgatgtcg gcgactgagc cggtcctgat cgactggaag
     3481 cctgtccccg cccacacatt tgtgccatcc gggtcacctg cgcgcaccga ggcggcccgc
     3541 agcggccctg tcagatagtg cacctcggga tatccgaacg gcgcctgtgc ctcgtggtcg
     3601 tcgacgaagc ggttgcgcag cccgcgcgcg tatcggccgg aaaacgattt cgtcacaacg
     3661 gtttcggtga accggggatc ctgcagagcg gcgcggtgca ccgggctgct gcccgattcg
     3721 tcggcgagca gaaacgccgt accgagctgg gccgcgaccg cgccctcctc gatcacgcat
     3781 ttgacgtcat cggcggtcac caggccaccg gccgcgatga ccgggacgtt cacccgggca
     3841 agtactgcga cgagcatctc ggacaacggt tgcgggttgg ggtctgcgac ggggtcgaag
     3901 gtgccgcggt gcccacccgc cgacgggcct tgcacggcga tcgcatcgac gccgcagtcc
     3961 actgccgcgt ccgcctcgta gaccgacgtc acggtgccga ccgtggcgat gcccgcatcg
     4021 cgcagccgcc tgcattcctc gacgctcggc agtccgaacg tgaacgacgc cacctcggga
     4081 cgcagctcga gcaggacgtc gagcttggcc gcccaggcgt cgtcgttgta ggaggggtcg
     4141 cccagctcgg ccccgtaccg ctcgacgtcg ggtgccaatg cggcggcgta cgtctcgacc
     4201 gcctcgggtg tggccgcgct gggctgcggg acgaacaggt tgacgccgag cgggccggtc
     4261 gtcagattgc gtgcggccgc tatgcgctcc gccaaagcgt cggcgctcag atagcccgcc
     4321 gcgacgaaac ccagtccgcc cgcattcgta ccggcggcgg cgagcgcggg cgtcgacgga
     4381 ccgcccgcca tcggcgcgac gatcaccgga accgcgaggt ctctgacatc gaaaccgttc
     4441 ggcatcatcg cctcccatcg cttcaacggc tcattcgtac cacgcgaacc tggatagcct
     4501 gccgccgtgg ccaggcagcc gatccagcgc gcggcccaac ggcgttgtcg cggccgcggt
     4561 gcgcggcatt gctacctgac ggacttcgcg aactgaagga cgtcctgcgg tgctgtcgtg
     4621 ctgatatttg gaaataccgt gtgccggacc agcttgccct cgaacgacag gcctgcgcgc
     4681 tccagaacgc gggccgaccg agtgttgtcg acgtgacaga tcgctgagac gcggtacacg
     4741 ctcgggtcgc tttccatctc ggcgagcagc aactggacaa cctccggcat cagccgctga
     4801 ccccaccatc ttcgaccgag tacatagccg aagtcacggc tgtgggcctg cactgtgcgg
     4861 aagccgcagg tgccgatgat ctccccggtg gcccgtaact cgatcagcca ggtccgttcg
     4921 gcgcccacgt tgaacaactc ggtgatcacc cggcgcgtct cgtctacgtc cagatgcggc
     4981 gtccatgaca tgtagcgggt gacttcggga tcggaggcga cggaggcgaa cagttcgtcg
     5041 gcgtcctgga gccgcggcgc tcgcaggccg acccgcgctc cagtcaggat tttcacgttg
     5101 gccacgccca gttcctgcac cttgtcgtgg atcggctgaa actggttgac tggtacatcg
     5161 ccatcacgtt caaccatacg atcgtgttct cacacgacaa gaaacagtcg gccaccttcc
     5221 tgaccgatct gttcgatctg ccgtgccctg cccctgtcgg ccggttcatg acggtgacgg
     5281 tctccaacgg cgtcacgctg gattacatgg atcttcccga aggtgagaac gtacgcccgc
     5341 agcactacgc gttcctggtc tccgaagacg aattcgatgc catctacggc aagatccgcg
     5401 cgcgtggcct cgagcactgg gcggatgcga tgagctctcg ccagggcact atcaacaccg
     5461 acgacggcgg gaagggcgtc tacttccgcg a
//
LOCUS       DASYLX010001084         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3150401,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001084 DASYLX010000000
VERSION     DASYLX010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150401"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGP27_25410"
     CDS             complement(<1..266)
                     /locus_tag="VGP27_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854674.1"
                     /translation="MAGDELDELYGVRPEEFTARRSELAAAAKKRGDAAAAKRMSAAR
                     KPTTAAWVVNQLVHTNEDVKRSLTDLGERLRAAHAAMDGAAIREL"
     gene            336..1253
                     /locus_tag="VGP27_25415"
     CDS             336..1253
                     /locus_tag="VGP27_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854675.1"
                     /translation="MVVAIDRPKLEGNVAVGEDRQIGFAEFGAPQGRAIFWLHGTPGA
                     RRQIPIEARAYAEEADIRLIGIDRPGIGSSTPHQYENVLEFAEDLRTIADVLGIGKMA
                     VVGLSGGGPYTLACAAAMPERVVAAGVVGGVAPTIGADGIGGGLMGNLGTRVAPILAV
                     AKVPIGMVATTLIRFIRPFGGPAADLYGLVSPPADRALLARPEIKAMFLDDLLNGSRR
                     QMAAPFYDVVAFARDWGFRLDEVKVHVRWWHGDADHIVPYAHGQHVVSRLPDAELFPM
                     PGESHLGGLGRAEEILRSMLEVWDRAGAD"
     gene            1257..>1567
                     /locus_tag="VGP27_25420"
     CDS             1257..>1567
                     /locus_tag="VGP27_25420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854676.1"
                     /translation="MRAIVLLAQSAALDQAGTLHALGLGWTASSTPVPQHALVIFVEV
                     EWAELNKSFPIRAELVDSDGVRVAHTEHGVIKAKHKPVHPEGTPVTIPFTATIPPLKL
                     TA"
BASE COUNT          255 a          543 c          517 g          252 t
ORIGIN      
        1 agctcgcgga tggctgcacc atccatcgcg gcgtgcgccg cgcgaagccg ctcccccagg
       61 tcggtgagcg aacgcttgac gtcttcattg gtatgcacga gctggttgac cacccatgcc
      121 gcggtggtgg gcttgcgggc cgcggacatc cgcttcgccg cagcagcgtc accccgcttc
      181 ttggccgcgg cggcgagctc gctgcgacgg gccgtgaact cctcgggcct gacgccataa
      241 agttcgtcga gctcgtcacc ggccacaacg caatgatgcg ccacgatgtc gggaagccgg
      301 gcagggactc gagagcggca tgcgaaactt tcggcatggt tgtcgcaatc gatcgcccga
      361 agctcgaagg aaacgtcgcc gtcggcgaag atcgccagat cggcttcgcc gagttcggcg
      421 caccgcaggg ccgggccatc ttctggttgc acggcacccc cggcgcccgc aggcagattc
      481 ccatcgaggc cagggcatat gcggaggaag cagacatccg gcttatcggc atcgaccggc
      541 cggggatcgg gtcgtcgacg ccgcatcagt acgagaacgt gctcgagttc gccgaggacc
      601 tgcgcaccat cgccgacgtc ctcggcatcg gcaagatggc tgtcgtcggt ctctccggcg
      661 gcggccccta cacactcgcg tgcgcagcgg cgatgcctga gcgcgtcgtg gcggcaggcg
      721 tggtcggcgg ggttgcaccc acgatcggag ctgacggtat aggaggcggc ttgatgggca
      781 atctcggaac ccgcgtcgca ccgatccttg cggtggcgaa ggttccgatc gggatggtgg
      841 ccaccacgct gatccgattc atccgtccgt tcggaggccc tgcggcggac ctgtacgggc
      901 tggtgtcacc gcctgccgac cgtgcactgt tggcgcggcc ggaaatcaag gccatgttcc
      961 tcgacgatct gctcaacgga agccgcaggc aaatggcggc cccgttctac gacgtcgtcg
     1021 ccttcgcgcg cgactggggc ttccggctgg acgaggtcaa ggtgcacgtg cgctggtggc
     1081 acggcgacgc cgaccacatc gttccctacg cacacggaca gcatgtggtg tcgaggctgc
     1141 ccgacgccga gctgttcccc atgcccggcg agagccatct cggcggcctc gggcgcgccg
     1201 aggagatcct gcgctcgatg ctcgaggtgt gggaccgcgc cggcgccgac tgacccatga
     1261 gagccatcgt gctgctcgcc cagagcgccg ccctggacca ggccggcacc cttcacgcgc
     1321 tcggacttgg ctggacggcc tcctcgacac ccgttccgca acatgctctg gtgatcttcg
     1381 tcgaagtcga atgggccgag ctcaacaagt cgttcccgat ccgcgccgag ctggtcgaca
     1441 gcgacggtgt ccgggtggcg cacaccgaac acggggtcat caaggccaag cacaaacccg
     1501 tacaccccga gggcaccccg gtcacgattc ccttcaccgc caccatcccc ccgctcaagc
     1561 tcaccgc
//
LOCUS       DASYLX010001085         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_515691,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001085 DASYLX010000000
VERSION     DASYLX010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515691"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VGP27_25425"
     CDS             <1..889
                     /locus_tag="VGP27_25425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEV7854677.1"
                     /translation="GFNRFYTRVLGLLRPKLAGSAFGLTEARVLFELAHAEQMAVGDL
                     RRALDLDAGYLSRILSRFIADGLVERRSASVDARRRLVRLTDAGQAAFAELDTLQADA
                     IDRMVEPLDENNRAELVTAMSRIRRVLDDEHTTTGLVLRAPGPGDLGWVVERHGTRYA
                     AEHGWDAGFEALVARIVADFGERRDATSEAAWIAELDGERVGCVFCTASGMPGTAKLR
                     LLLVEPQARGAGVGSRLVDECLRFARRSGYTRITLWTNDVLAAARRIYQRAGFQLDRS
                     EPHTSFGRDLVGEYWSRDL"
     gene            complement(891..1841)
                     /locus_tag="VGP27_25430"
     CDS             complement(891..1841)
                     /locus_tag="VGP27_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV7854678.1"
                     /translation="MKVFRRFFSAAAAATVLAGLIGVLGGTPTAAAAPEVLSVPSPSM
                     GRNIQVQFQGGGPHAVYLLDGLRARDDRNGWDIETGAFSWFDGSGLSVVMPVGGMSSF
                     YTDWYRPAVGNGTTQTYKWETFLTSELPGWLAANKGVSPSGNAVVGLSMSGGAALVLA
                     AYHPGQFVYAGSLSGFLNTSNSLWPVLIGIAMADAGGFNAVDMWGPYGGPAWQRNDPM
                     LQIGRLVSNGTRIWVYSGNGNPTDLDGGLGNAAIPAQFLEGLTINTNKEFQARYQRAG
                     GANGVFDFPPNGTHSWGYWGGQLQAMKPDIQRVLGATPAV"
     gene            complement(1973..2221)
                     /locus_tag="VGP27_25435"
     CDS             complement(1973..2221)
                     /locus_tag="VGP27_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854679.1"
                     /translation="MTVSTAHRVIDAAESGQPATVSALLRQLGVAHTCAEKQKAALAP
                     WMMLHEAQQPLRISLLRNGYGLLLKEIDAKRAGPAALV"
     gene            complement(2367..2654)
                     /locus_tag="VGP27_25440"
     CDS             complement(2367..2654)
                     /locus_tag="VGP27_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEV7854680.1"
                     /translation="MVIVAGHLIVAPEVRDGYLAGCADVVRQARRAPGCLDFAITADL
                     LDPARINIFERWNSQDAVDAFRGSGPSADQADRVVTASVSEYGVTGERGLT"
     gene            2739..3077
                     /locus_tag="VGP27_25445"
     CDS             2739..3077
                     /locus_tag="VGP27_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854681.1"
                     /translation="MKLRDALDSIVPDPAAAHVYGQPYQLEDGVTVVPVAKVRGRTKG
                     ATDTDLRLAANPVGVFVVKNGEAAWVPAVDSTRIALMGELIGLIAATFATLAMVRRPP
                     WPDLHGTVAK"
     gene            complement(3025..3927)
                     /locus_tag="VGP27_25450"
     CDS             complement(3025..3927)
                     /locus_tag="VGP27_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEV7854682.1"
                     /translation="MQQDLVGLAFAAGLVAALNPCGFAMLPAYLALVVRNSDEGDAAS
                     AVGRAVGATAAMTLGFLAVFGTFGFLTVSVASTVQRYMPYVTVLVGIMLVALGIWLTS
                     GRQLIILLPNRLADNASRAPTRRLGSMFGYGVGYAIASLSCTVGPFLAVTGASVGATS
                     TIGRLWVYVAYAAGFALVVGALAVAVALAGSALVERMRRILPYVNRIGGAVLILVGIY
                     VSYYGYYEVRLFTANGNPADPVIAAAGRLQAAIAGWVHQHGAWPWIAVLGVLVLTGAL
                     WGPARSSATSRRSRADRATAADEP"
BASE COUNT          692 a         1315 c         1299 g          642 t
ORIGIN      
        1 cggtttcaac cgcttctaca cccgggttct cgggttgttg cgaccgaagc tggcgggctc
       61 tgcgttcggc ctgaccgagg cccgtgtcct gttcgaattg gcccacgccg agcagatggc
      121 tgtcggcgac ttgcgccgcg cactcgacct cgatgccgga tacctgagcc gcatcctgtc
      181 gagattcatc gccgacggcc ttgtcgaacg ccggtcagcg tcggtcgacg cgcgtcgcag
      241 actggtgcgc ctgacggatg ccggccaggc ggcgtttgcc gaactcgaca cactccaggc
      301 tgacgcgatc gaccgtatgg tcgagccgct tgacgagaac aaccgtgccg aactcgtgac
      361 cgcgatgagc cggatccggc gggtgctgga cgacgaacac accaccacgg ggctcgtcct
      421 gcgcgcgccg ggaccgggcg acctcgggtg ggttgtcgag cgtcacggca cccgctacgc
      481 cgctgaacat ggctgggacg ccggcttcga ggcgctggtc gcccgtatcg tcgccgactt
      541 cggtgagcga cgcgacgcca catcggaagc cgcgtggatt gccgagctcg acggcgaacg
      601 ggtcggttgc gtcttctgca cggcctccgg gatgcccggc accgcaaagt tgcgtctact
      661 gctcgtcgaa ccccaggcgc gtggcgcagg cgtcggcagc cggttggtgg acgagtgcct
      721 gcggttcgcc cggcggtccg ggtacacgcg aatcacgttg tggaccaacg atgttctggc
      781 tgcggcccgg cggatctacc aacgggccgg cttccagctc gatcgaagtg agcctcacac
      841 cagtttcggc cgtgacctcg tcggtgagta ctggtcgcgc gacttgtagg tcatactgcc
      901 ggcgtggcgc cgagcacgcg ttggatgtca ggcttcatgg cctgcaactg accgccccag
      961 tagccccagc tgtgggtgcc gttgggtggg aagtcgaaaa ccccgttggc gccgcctgcg
     1021 cgttggtagc gggcctggaa ctccttgttg gtgttgatcg tcaatccctc gaggaactga
     1081 gccggaatcg cagcgttgcc cagaccgccg tcgagatcgg tcggattgcc gttgccgctg
     1141 tagacccaga tccgggtccc gttggagacg agccgtccta tctgcagcat gggatcgttg
     1201 cgttgccaag ccgggccacc gtaggggccc cacatgtcga cggcgttgaa gccgcccgca
     1261 tcggccatag cgatgccgat cagcaccggc cacaaactgt tcgacgtgtt gaggaaaccg
     1321 gaaagcgacc cggcgtagac gaactgacca gggtggtagg cagccagaac cagggccgca
     1381 ccaccggaca tcgagagccc gaccaccgcg ttgccggacg ggctgacacc cttgttggcc
     1441 gcgagccaac ccggaagctc cgatgtcagg aacgtctccc acttgtaggt ctgggtggtg
     1501 ccattgccga ccgcgggccg gtaccagtcg gtgtagaagc tcgacatgcc gccgaccggc
     1561 atcaccaccg agagcccgga cccgtcgaac cagctgaacg cgccggtctc gatgtcccac
     1621 ccgttgcggt cgtcccgcgc gcgcagtccg tcgaggagat acaccgcatg gggtccgccg
     1681 ccctgaaact gaacctgaat gttgcggccc atcgacggtg acggcacgct cagtacctcg
     1741 ggtgccgcgg ccgccgtcgg cgtgccaccg agcacaccga tcaggcctgc gagaacggtt
     1801 gccgcggctg cggcggagaa gaatcggcgg aaaactttca tggcattact tcctgacatg
     1861 cgtagcgccc cgacaacctg aacatcgtcg atcaggaggc agcagttaca ggattcgatc
     1921 gagcagacaa cagccatccc agtcggggat gacgacaacg tcgacgctga ggtcagacca
     1981 gggcggccgg cccggcgcgt ttcgcgtcga tctccttcaa cagcaggccg tagccgttcc
     2041 gcaacaggct gatccgcagc ggctgctggg cttcgtggag catcatccac ggcgccaggg
     2101 cggccttctg cttctcggcg catgtgtggg ccactccgag ctgtcggagc aacgcgctca
     2161 cagtggcggg ttgccccgac tcggcggcgt cgatcacgcg atgagctgtt gagacggtca
     2221 tggctgtccc cgttgtctcg atcgggcgcc tttcggtggc agttcttatt ggcgcctctg
     2281 ccgataaagt gtaggacgat gttgctgctc gggtggggca atttcgcatg ctggatgtta
     2341 agccgccgac ccaagttgcc agcggcctag gtgagtcctc gctcgccggt cacgccgtac
     2401 tccgagaccg aggctgtcac gaccctgtcg gcctggtctg cgctgggacc gctcccacga
     2461 aacgcgtcca cggcgtcttg ggagttccat cgttcgaaga tgttgatgcg agccggatcc
     2521 agcaaatcgg ctgtgatcgc gaagtcgaga cagccgggcg cacggcgggc ctgccggacg
     2581 acgtccgcac agcccgcgag atagccatcg cgcacctccg gggccacgat cagatgccct
     2641 gcgacgatca ccatggcgtg ctcctctctc gttgggtcgg tagaactgag accagccaag
     2701 acccatcgag tcatcgttgg aaaccgaagg gaacgcgaat gaaactgcga gatgctctcg
     2761 actcgatcgt gcccgacccc gccgccgccc acgtctacgg gcagccgtac caactcgaag
     2821 acggtgtgac ggtcgtgccg gttgccaaag tgcgcgggcg gaccaagggc gcgaccgaca
     2881 cggacctgcg cctggcggcg aatcccgtcg gggtgttcgt cgtcaagaac ggtgaagcgg
     2941 cctgggttcc cgccgtcgat tcgacccgca tagcgttgat gggcgagttg atcgggctca
     3001 tcgctgcgac tttcgcgacg ctggctatgg ttcgtcggcc gccgtggccc gatctgcacg
     3061 ggaccgtcgc gaagtagcgc tgctcctcgc gggcccccag agcgccccgg tcagtacgag
     3121 caccccgagc accgcgatcc acggccatgc gccatgctga tgaacccagc ctgcgatggc
     3181 cgcctgaagg cgaccggccg ccgcgatgac cgggtccgcc gggttgccgt tcgcggtgaa
     3241 cagcctcacc tcgtagtagc cgtaatagct cacgtagatg ccgaccagga tcagcaccgc
     3301 gccgccgatt cggttgacgt agggcagtat ccgtcgcatc cgctccacga gtgccgagcc
     3361 ggccagtgcg accgcaacgg ccagcgcacc gacgaccaac gcgaacccgg ccgcgtatgc
     3421 gacatacacc cacagcctgc cgatcgtgga tgtggcgccc acactcgcac ctgtcaccgc
     3481 gagaaagggg ccgacggtgc aggacagcga ggcgatcgca tagccgacgc catagccgaa
     3541 catcgatccg agcctgcggg tgggcgcccg ggaagcgttg tcggccaacc ggttcggcag
     3601 caatattatc agctgacggc ccgacgtcag ccagatgcca agcgccacaa gcatgatgcc
     3661 gaccagcacg gtgacgtagg gcatgtatct ctgaaccgtc gaggccacag agaccgtgag
     3721 gaacccgaat gtgccgaaca cggcgaggaa gccaagcgtc atggcggcgg tggccccgac
     3781 ggcgcggccc accgcgctcg cagcgtcccc ctcgtcgctg ttccgaacca ccagcgcgag
     3841 gtaggcggga agcatcgcaa aaccgcacgg gttcagcgcg gccaccaacc cggcggcgaa
     3901 cgccagcccg accagatcct gctgcacgcg gcgcgccggt tacgcggt
//
LOCUS       DASYLX010001086         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2600301,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001086 DASYLX010000000
VERSION     DASYLX010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600301"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1612)
                     /locus_tag="VGP27_25455"
     CDS             complement(113..1612)
                     /locus_tag="VGP27_25455"
                     /inference="COORDINATES: protein motif:HMM:NF009120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7854683.1"
                     /translation="MNPKRKQTRLRPAHLAIAGLAALIAAGDHVGATDRRSERSVEFI
                     QSRTAGQPIMAVVSLHNQRITVYDANGWILRAPVSSGQKGRETPAGIFSVIQKEAEHY
                     SNLYDDAYMPHMQRITWSGIALHGGPLPGYPASHGCVRMPFDFAARLFDATKLGLRVI
                     VAPTDAAPIEIAHPVLFLSKPGAGALAAARTAEADEAARKADQARLAAGTAFRESMRA
                     MVPVRALENLKLRAEAQLAAAETALGSAISADAKEQAERATAQAVARIAELQVQWAAA
                     KAELQPKLDAVTSAREAAVVAETARVAAAEAARQVARELEPVSVLISRKTQRLYVRQA
                     FESILESPVTILDGDSPIGTHVFTAMERTNSDTNMRWSVVSLDGGRPHGGTVELHGRG
                     RAGVGGDGEPMRTDPNSAKAALDRIVIPQDALDRIAGMAPRSSLIVTDEALSSETGKG
                     TDFVVLLSGEPQGGIKSRRRSPGAEFGYARPRDRLPFWRSPFAGPFSTW"
     gene            complement(1962..>2080)
                     /locus_tag="VGP27_25460"
     CDS             complement(1962..>2080)
                     /locus_tag="VGP27_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854684.1"
                     /translation="EHIQRIYEMCNRNVSETARRLNMHRRTLQRILAKRAPR"
BASE COUNT          378 a          683 c          638 g          381 t
ORIGIN      
        1 ccgaaaataa cgtgttcgat cgaggccgct gacacgacac cgattggcgc tccctttggc
       61 cttgtgtcat cgggatttat gtacgccgtt actggaagtg aaaagaacag gctcaccagg
      121 tggaaaatgg acccgcgaaa ggcgagcgcc aaaaaggcag gcgatcgcgt ggacgggcat
      181 agccgaattc ggctcccggg ctgcgccgtc ggctcttgat gcctccttgc ggctcgccgc
      241 tcaacagcac cacgaaatcc gtgcccttac cggtctccga gctcaacgcc tcgtccgtga
      301 cgatcaggga agatcgcggt gccatcccgg cgatgcggtc caatgcgtcc tgcggaatga
      361 cgatgcgatc gagcgcggcc ttcgcgctgt tcgggtccgt ccgcatcggc tcaccgtcac
      421 caccaacgcc cgcgcggccc cggccatgta gctcaaccgt gccgccatgc ggacgtccgc
      481 cgtccaacga gacgacgctc catcgcatgt tggtgtcgct gttggtccgc tccatggcgg
      541 tgaagacgtg cgtgccgatc ggactgtcgc catccaggat cgtgaccgga ctctccagga
      601 tggactcaaa ggcttgccgg acataaagcc gttgggtctt gcggctaatc aacaccgata
      661 ccggctcgag ttcgcgcgcc acctggcgcg ccgcctcggc tgctgcgacc cgcgcagtct
      721 ctgcgacgac ggcggcttca cgcgcagacg tgacggcatc gagcttcggt tgcaactcgg
      781 ccttggcggc cgcccattgc acctgcaact cggcgatcct ggcgactgcc tgcgccgtgg
      841 cgcgctcagc ctgctccttt gcgtcggccg agatggccga gccaagcgca gtctcggcgg
      901 ccgccaattg cgcctcggct ctgagcttca gattttccaa cgcgcgcacc ggcaccatcg
      961 cccgcatgga ttcccggaac gccgtcccgg cggcgagtct cgcctgatcc gccttcctgg
     1021 ccgcctcatc tgcctcggcg gtacgggccg cggccaaggc accggcaccc ggcttcgaca
     1081 gaaacagaac cggatgggct atctcaatcg gcgccgcatc ggtcggtgcc acgatcaccc
     1141 gcaagcccag cttggtcgcg tcgaacaagc gggctgcgaa gtcgaagggc atccgaacgc
     1201 agccatggga cgccggatat cctggcaggg ggccgccgtg gagcgcgatg cccgaccagg
     1261 tgatgcgttg catgtggggc atgtaggcat cgtcatacag attcgagtag tgctccgctt
     1321 ctttttggat gacgctgaag atcccggcgg gcgtctcgcg tcccttttgg ccgctcgaca
     1381 ccggcgcccg gaggatccag ccattggcgt catagacggt gatccgctgg ttgtggagcg
     1441 aaacgaccgc catgatcggc tgaccggctg tgcgcgactg gataaactcg accgagcgtt
     1501 cgctccgacg gtctgtggcg cccacatgat ctccggccgc gatcagcgcg gccagacccg
     1561 caatggcaag atgcgcgggc cgcagccttg tctgcttgcg cttcgggttc acaaaacgag
     1621 acctcatgcg cgtcccatct gatcggggct acgccagctt gcactcaggc gattcataac
     1681 gccattaggt cgaacctgcc aactcggaac ttcccgaatg tgctcataga tcggtcggag
     1741 gcagcatcct taggaagctt aacccttgta gcaacatccc tgagctcctg tccggcttgg
     1801 tcgatgtcgg ttccggggtc aaaagccgaa atcctgcctc aaaatgacgt cagccttggc
     1861 tcgaacagcg gacattgttc gacgcgatcg acaagccgga aagttgcctt aaccgtcgac
     1921 gagcgaaggc ggaagcgctc aagcaatcca gaacgcaaaa actaccgcgg cgcgcgcttg
     1981 gccaggatgc gctgcagggt gcggcgatgc atgttcagcc gccgcgccgt ttcagagacg
     2041 ttacggttgc acatctcgta gatgcgctgg atgtgctccc
//
LOCUS       DASYLX010001087         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1615693,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001087 DASYLX010000000
VERSION     DASYLX010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615693"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VGP27_25465"
     CDS             <1..836
                     /locus_tag="VGP27_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV7854685.1"
                     /translation="RRMTTRVACAQVPLCIGDTAGNRATCRTAVESAARDGAQIVVLP
                     ELASSGYVFADRAELSSLAETRDGPAISEWADLAEAFGLTIVAGFPEAAGDAVYNSAA
                     VVDPTGLRGVYRKAHLWDNENAVFDRGDDLPMVVDTDHGRIGVMVCYDVEFPEWVRAV
                     ALSGADLLCAPVNWPLLPRPEGERPTEMVRALAGAGMNRLPMAVCDRVGTERGQAWIG
                     GSVVIDADGYPLATAGFGTADVVTADVDLAESRVKRINAHNDVLGDRRTDLYRRTALL
                     D"
     gene            complement(825..1490)
                     /locus_tag="VGP27_25470"
     CDS             complement(825..1490)
                     /locus_tag="VGP27_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7854686.1"
                     /translation="MHRTTPSTKVSRLGEKQTTSRSRLDELLDSTPLATVALVRDGHP
                     VLFPTGFARIDDQFVIHGSTGSPWMRALATGAPAAVSVTGLDGIMVARSGFESSFRYR
                     SAVVFGVFEQVPDADKIRYLERLTEAFIPGRTEELRASTRKELAATLPLRLPIRADNW
                     SLKISAGWPEDPDEDVAGDAWAGVIPMSVAYGAPLPAPDLRPGIPVPGSVRAMTGGRS
                     SVQ"
     gene            complement(1502..>1803)
                     /locus_tag="VGP27_25475"
     CDS             complement(1502..>1803)
                     /locus_tag="VGP27_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7854687.1"
                     /translation="VKSYPNSFVQTDLDQRLKAANASNLVLAGFMTHMCVNSTARGAF
                     NLGYAPTVVAAATATRALPGVDGDPVPASTLHSASLAAIADLFAVVVVRTSEIPD"
BASE COUNT          279 a          602 c          633 g          289 t
ORIGIN      
        1 atcgacggat gaccactcgc gtcgcctgcg cccaagtccc gctgtgcatc ggagatacgg
       61 ctggaaatcg cgccacctgc agaaccgctg tcgaatctgc cgcccgcgac ggcgcgcaga
      121 tcgtcgtgct gccagaactc gcgtcgtcgg gttacgtctt cgccgatcgt gccgagctgt
      181 cgtcgttggc ggaaacacgg gatggcccgg cgatcagcga gtgggccgat cttgcggagg
      241 cgttcgggct caccattgtc gcgggcttcc cggaggcggc gggtgacgcc gtctacaaca
      301 gcgcggccgt cgtcgacccg accggtctgc gtggcgtcta ccgcaaggcg cacctgtggg
      361 ataacgagaa cgccgtgttc gaccgcggcg acgacctgcc gatggtcgtc gacacggacc
      421 acggtcgcat cggcgtgatg gtctgttacg acgtcgagtt cccggaatgg gttcgcgccg
      481 ttgcgctttc cggtgccgac ctgctgtgcg caccggtgaa ctggccactg ctgccgcggc
      541 cggagggcga acgtccgact gagatggtcc gggcgctggc gggcgcgggg atgaaccggc
      601 tgccgatggc ggtatgtgac cgcgtcggca ccgaacgcgg ccaggcctgg atcggcggca
      661 gcgtcgtcat cgacgccgac ggctacccgc tcgccaccgc cgggttcggc acggcggacg
      721 tcgtcaccgc cgatgtcgac ctcgccgaat cgcgggtcaa gcgcatcaac gcgcacaacg
      781 acgtgctcgg cgaccggcga acggatcttt accgccgcac cgcgttattg gactgacgac
      841 cgcccgccgg tcatcgcgcg caccgatccg ggcaccggga tgccaggcct caggtcgggt
      901 gcgggcagag gggcgccgta ggcgacagac atcgggatga cacccgccca tgcgtcgcct
      961 gcgacgtcct cgtccgggtc ctccggccaa ccggcgctga tcttcagcga ccagttgtcc
     1021 gcgcggatgg gcaggcgcag cggcagtgtc gcggcgagct ccttacgcgt gctggcccgc
     1081 agttcttcgg tccgcccagg gatgaacgct tcggtcagcc gctccaggta ccggatcttg
     1141 tcggcatcgg gcacctgctc gaagacgccg aagacgacgg cgctgcgata gcggaatgag
     1201 gattcgaacc cgctgcgcgc caccatgatt ccgtccaagc ccgtgaccga gacggcggca
     1261 ggcgcgcctg tggccaacgc gcgcatccac ggtgagccgg ttgacccgtg gatgacgaat
     1321 tggtcgtcaa tcctggcgaa gccggtcgga aagagcaccg gatgaccgtc gcggacgagc
     1381 gccacggtgg ccaggggtgt gctgtcgaga agctcgtcga gtcggctgcg cgatgtggtc
     1441 tgcttctcgc cgagccggct gaccttggtc gagggtgtgg tcctgtgcac cgccgggccc
     1501 gtcagtccgg gatttccgac gtgcgcacaa cgacgacggc gaagaggtcg gcgatcgcgg
     1561 ccaggctcgc cgagtgcagc gtcgatgcgg gcaccggatc gccatcgacg ccgggcagcg
     1621 ccctggtggc ggtcgcggcg gccaccaccg tcggcgcgta ccccaggttg aacgccccgc
     1681 gcgccgtgga gttcacgcac atgtgtgtca tgaatccggc gagcacgaga ttcgacgcgt
     1741 tcgccgcctt caatcgctgg tcaagatccg tctgcacgaa cgagttcggg tagctcttca
     1801 cca
//
LOCUS       DASYLX010001088         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1615906,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001088 DASYLX010000000
VERSION     DASYLX010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615906"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGP27_25480"
     CDS             complement(<1..467)
                     /locus_tag="VGP27_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV7854688.1"
                     /translation="MSIRRTDRMREVLSLLRERGEVDSQVLRKELRVSAATLRRDLGE
                     LEEQGLLVRTHGGARALDPSGSEIPVRLRDHRMVAIKRRIAQHAAALVPAGPQAVALT
                     GGVTTGEVARSLKGRPQMTIVTNSLTIAADCAVDAHMKVIMTGGLVRANSMEAV"
     gene            597..>1604
                     /locus_tag="VGP27_25485"
     CDS             597..>1604
                     /locus_tag="VGP27_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEV7854689.1"
                     /translation="MTSVATAQAPRRVNWLADTISRHKAGEAVGIYSICSAHPTVVEA
                     AMAQAAADGSYVLIEATSNQVDQFGGYTGMRPPEFRDMVLAIADERGFPSDRVVLGGD
                     HLGPNRWQRENASAAMQKAETLIAAYVEAGYAKIHLDCSMSCADDPTVLDDEVVAQRT
                     ARLMRVAEDTARRTGVGAPMYVMGTEVPVPGGAHETLGVLTPTPADRARRTIATHRKA
                     FADAGLDHVWPRVVALVVQPGVEFDHLHVIDYQRDATVELRRVLDDEDHLVFEAHSTD
                     YQRPAQLRELVEDHWAVLKVGPGLTFAMREALFALALIENELVPRPSRSNLIEVVERR
                     ML"
BASE COUNT          263 a          618 c          462 g          261 t
ORIGIN      
        1 accgcctcca tcgaattcgc cctcaccagg ccgcccgtca tgatcacctt catgtgagcg
       61 tccacagcgc agtccgccgc gatcgtcagc gagttcgtca cgatcgtcat ctgcggccgc
      121 cccttcagcg atcgcgccac ctcccccgtc gtcaccccac ccgtcagcgc caccgcctgc
      181 gggcccgccg gcaccagcgc cgccgcgtgc tgcgcgatcc gccgtttgat cgcgaccatc
      241 cgatgatccc gcagccgcac cgggatctca cttccgctgg ggtccagcgc cctcgctccg
      301 ccatgcgttc gcaccaacag cccctgctcc tcgagttcgc ccagatcgcg gcggagcgtc
      361 gccgccgaca cccgaagctc ttttcgcaac acttgcgagt cgacctcgcc gcgctcgcgc
      421 agaagtgaca gcacctcacg catccggtcg gttcgcctga tcgacatgac ggcgactcct
      481 cccaatcagt gcaaacgatc aattttagtg atcacttggc ggctatccat tgcgcactat
      541 gagcgagctt ctcatgatcg acgaatgttg catacgttcg gccgccgggt caccgggtga
      601 cgtcggtcgc caccgcgcag gccccgcgac gggtcaactg gctggccgac acgatcagtc
      661 ggcacaaagc cggtgaggcc gtcgggatct actccatctg ctccgcccac cccaccgtcg
      721 tggaggccgc catggcgcaa gccgccgccg atggtagcta cgtcctcatc gaggccacgt
      781 cgaatcaggt tgaccagttc ggcggctaca ccggtatgcg tccgcccgag ttccgcgaca
      841 tggtgctcgc catcgccgac gaacgcgggt tcccatctga ccgtgttgtc ctcggcggcg
      901 accatctcgg ccccaaccga tggcaacgcg aaaatgccag cgccgcaatg cagaaggccg
      961 agaccctcat cgccgcctat gtcgaggccg gttacgccaa gatccacctc gactgctcga
     1021 tgtcctgtgc cgatgacccg acagtcctcg acgacgaggt cgtcgcgcag cgcaccgcgc
     1081 gcctgatgcg cgttgccgag gacaccgccc gccgcaccgg cgttggcgcg cccatgtacg
     1141 tcatgggcac cgaggtgccc gtccccggcg gcgcccacga gaccctgggc gtcctcaccc
     1201 cgacccccgc cgaccgcgcc cgtcgcacaa tcgccacgca ccgcaaagcc ttcgccgatg
     1261 ccgggctcga ccacgtctgg ccgcgcgtcg tcgcgttggt ggtgcagccc ggcgtggagt
     1321 tcgaccatct ccacgtcatc gactaccagc gcgacgccac ggtcgaactg cggcgcgtcc
     1381 tcgacgacga ggaccacctg gtcttcgaag cgcactccac cgactatcag cggcccgcgc
     1441 agctgcgcga actggtcgaa gatcactggg ccgtcctgaa ggtcggaccc ggcctgacct
     1501 tcgcgatgcg cgaagcactg ttcgcgttgg cgctcatcga aaacgagctc gtaccgcggc
     1561 cgtcgcggtc caacctgatc gaggtcgtcg agcgcaggat gctc
//
LOCUS       DASYLX010001089         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2271066,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001089 DASYLX010000000
VERSION     DASYLX010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271066"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VGP27_25490"
     CDS             <1..643
                     /locus_tag="VGP27_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7854690.1"
                     /translation="TADSGSSTNWFARCVKLRSGMRASVSGTLATMGCAVPYAIGAKF
                     AHPSRPVIALVGDGAMQMNGLAELLTIRRYRDRWADARMVICVFHNNDLNQVTWELRA
                     MGGAPKFEESQSLPEVSYADVARSMGLRSISVDTDDAVAGAWDEALSYDEPVLLDVTC
                     DPEVPPIPPHATYEQIKEMTSAVLKGDPGGWHLMYQGAKTKLQELIPGGRSTS"
     gene            640..1212
                     /locus_tag="VGP27_25495"
     CDS             640..1212
                     /locus_tag="VGP27_25495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854691.1"
                     /translation="MSEAGLVVVGSGPAGVSAAEAFRRHRVDSPVRILTADPAPPYAR
                     PPLSKEFLRGERDDVALHPAQWDAENRLELVRARVTRIDTAARTVVVGEERVAYSALI
                     LACGSAPTRLPVPGGEHAYQLRSLADAQRLRDAGVGSQSAVVIGAGFIGCEAAASLAL
                     RGVSAPWWRRTRHPRTSGSAATPMSGCVGW"
     gene            1140..1787
                     /locus_tag="VGP27_25500"
     CDS             1140..1787
                     /locus_tag="VGP27_25500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854692.1"
                     /translation="MAPNAAPQDKRLGGDADERLRGLVTATGARYVGGVSVEEIRHGA
                     VRLDNGVTIDCDLVLAATGVRPQGALADEAGLRMTDSRIVAGADMRTSAPDVFASGDV
                     ASAFNATARRHISVEHWQDAVDRGEIAGAAAAGVDGQWDGVPGFWTTIGDATVKYHAW
                     GDGYDRSRLVDHGDGFTAWYERDGATVGVLTLNADDDYERGETLIKNGQPVCAPG"
     gene            1824..2081
                     /locus_tag="VGP27_25505"
     CDS             1824..2081
                     /locus_tag="VGP27_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor"
                     /protein_id="HEV7854693.1"
                     /translation="MPNSSIKNEKMYEDLRKEGNSKQKSARIANAAAASGKSAVGKKG
                     GKSGSYADWTVADLKKRAKEIGLSGYSSLSKDKLISKIRNS"
     gene            2172..2414
                     /locus_tag="VGP27_25510"
     CDS             2172..2414
                     /locus_tag="VGP27_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV7854694.1"
                     /translation="MATGETGFDDVTYDLISVQYHSLKAGHDYGQYVRDAKNAGLDDV
                     ASFFETVMAQDSERARRCHELLGRMQGSTVSGPAIS"
     gene            2634..3308
                     /locus_tag="VGP27_25515"
     CDS             2634..3308
                     /locus_tag="VGP27_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEV7854695.1"
                     /translation="MYRVLELVEALQRKAATDFQAVLREINQSTVASVPGAQYAGVTL
                     VDAERGVSTLAPTHLFPAYLDDVQREVDEGPCLSAAWEQHTIRITDLAADDRWPKYRA
                     AALKRTPVRSIVSFQLFNDAKSIAALNLYAEPACAFDDESIELGLIYAAHTTVAWNAM
                     RGHEQFSSALASRDLIGQAKGMLMERFDIDALAAFELLRKLSQESNIKLIDIAAKLVQ
                     GERPAE"
     gene            complement(3315..>4196)
                     /locus_tag="VGP27_25520"
     CDS             complement(3315..>4196)
                     /locus_tag="VGP27_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854696.1"
                     /translation="MAGRLEGKVAFITGGARGQGRAHALAMAKEGADIIVVDICRNID
                     SNPYPLATPDDLSDVERSVKELGRRVYARIADVRERHELRDALEAGVADLGRLDIVVA
                     NAGILPMAFGKEFDPMHFVDATDVDLLGVMNTVAVALPHLPNGASVIITGSTAGMIKG
                     TSDNPMMGPGGAGYGWSKRVLIDYVDHLCLQVAPKMIRVNAMHPTNVNTALLQNDGIY
                     SVFRPDMMAEGKKPTREDAEPAFTYFNAMPIPYVEPEDIANLAVFLASEESRYITGQH
                     IRVDAGSMLKWPNGPGA"
BASE COUNT          755 a         1316 c         1414 g          711 t
ORIGIN      
        1 gaccgcggac tccggttcgt cgaccaattg gttcgcccgc tgcgtcaagc tgaggtccgg
       61 catgcgggcg tcggtatcgg gcaccctggc caccatggga tgtgcggtgc cgtacgcgat
      121 cggcgccaag ttcgcccatc cgtcgcggcc ggtgatcgcc ctcgtgggag atggggccat
      181 gcagatgaac ggcctcgccg agttgctgac gattcgccgc tatcgcgacc ggtgggccga
      241 tgcgcggatg gtgatctgcg tgttccacaa caacgatctc aaccaggtga cctgggagtt
      301 gcgcgcgatg ggcggcgcac cgaaattcga ggagtcgcag tcacttcccg aagtgtccta
      361 cgccgacgtg gcgcgttcca tggggctgcg gtcgatctcc gtcgacaccg acgacgcggt
      421 cgccggggcg tgggacgagg cactgtccta cgacgagccg gtgcttctgg atgtgacctg
      481 cgatcccgag gtgccgccca ttccgccgca tgccacctac gagcagatca aggagatgac
      541 gtcggcagtg ctcaagggtg accccggcgg ttggcatctg atgtaccagg gcgcgaaaac
      601 caaactgcag gaacttattc cgggtggacg gtcgacgtca tgagcgaagc cggactcgtt
      661 gtcgtcggaa gcgggcccgc gggtgtgagc gccgctgaag catttcgccg gcaccgggtc
      721 gactcgcccg tgcgaatcct cacggccgat cccgctccgc cgtacgccag gccgcccctg
      781 agcaaggagt tcctacgcgg cgaaagggac gacgtcgccc tgcaccccgc gcagtgggat
      841 gccgagaacc gcctcgagtt ggtgcgcgca cgcgtcaccc gcatcgatac tgctgcgcgc
      901 accgttgtcg tcggcgaaga gcgcgttgcg tattccgccc tgatactggc gtgcggatcg
      961 gcacccaccc gccttccggt gcccggcggt gagcacgcct atcagctgcg ctcgctggca
     1021 gacgcgcagc gcctacggga cgccggcgtc ggctcgcaat ccgctgtcgt catcggtgcg
     1081 ggtttcatcg gctgtgaggc tgcggcatct ctcgccctcc gcggtgtttc ggcaccttgg
     1141 tggcgccgaa cgcggcaccc caggacaagc ggctcggcgg cgacgccgat gagcggttgc
     1201 gtgggctggt gacggcgacc ggcgcacggt acgtcggtgg cgtctcggtc gaggagatac
     1261 ggcatggcgc ggtgcgactg gacaacggtg tcaccatcga ctgcgacctc gtactcgccg
     1321 cgacgggcgt gcggccacag ggcgcgctgg ccgatgaagc gggcctgagg atgaccgatt
     1381 cacgcatcgt ggcgggtgcc gatatgcgca cctcggcgcc ggatgtgttc gcgtcgggcg
     1441 acgtcgcgtc ggccttcaac gccaccgcca gacgacatat ctctgtcgag cattggcagg
     1501 atgcggtcga ccggggcgag atcgccgggg ccgcggcggc aggcgtcgac ggacaatggg
     1561 atggcgtgcc gggattctgg acgacgatcg gcgacgccac cgtcaagtac cacgcctggg
     1621 gtgacggcta cgaccgtagc cggctggtgg accacgggga cggtttcacc gcgtggtacg
     1681 agcgggacgg cgcgaccgtc ggggtgctga cattgaacgc ggacgacgac tacgaacgcg
     1741 gcgagaccct gatcaagaac ggccaacccg tttgcgcgcc tggctaaacg ggcacttcat
     1801 tcccatcgga aggaggcgac accatgccca actcatcgat caaaaacgag aagatgtatg
     1861 aggacctgcg caaggaaggc aattccaagc agaagtccgc tcgtatcgcc aacgccgcgg
     1921 cggccagcgg caagtcggcc gtcggcaaga agggcggcaa gtccggttcc tacgccgact
     1981 ggaccgtggc cgacctgaag aagagggcca aggaaatcgg cttgtctgga tactcgagcc
     2041 tcagcaagga caagctgatc tccaagatcc gcaattcctg acggggaatc actcaccgcg
     2101 catcgtctgc cgtttatctc gtctgccgcc gggtagtcgg cacccatgca cgcccgatga
     2161 gaggagacga catggctacg ggtgaaacgg gattcgacga tgtgacgtac gatctcatct
     2221 ccgttcaata ccactcactg aaggccggtc acgactacgg ccagtatgtg cgcgatgcga
     2281 agaacgcggg cttggatgac gtcgcatcgt tcttcgagac ggtgatggcc caagactcgg
     2341 agcgggcccg ccgctgccac gaattgctag gcaggatgca aggatccacg gtctccggac
     2401 ccgcgatcag ctgatccgga aaacgaagaa ctgcggtctc gccgcggccg gaatgcgaag
     2461 agagcatttg ctgtcgccgg tgaagccggt tgccgagcgg ccgaggcggt gggatagtcg
     2521 catcagaagg ggtcgccccc cgggagggtg gattattgcc tcgccacgaa gagagcgcct
     2581 ggcgcacaac cacaggcgga ttgggtcacg gtatttcgag cgaacgtcgg cacatgtacc
     2641 gcgtgttgga actggtcgag gcgttacaac gtaaagccgc gaccgacttc caggctgtgc
     2701 tgcgcgagat aaatcagagc acggtggcgt cggtacccgg ggctcagtac gccggcgtca
     2761 ctcttgtcga cgccgaaaga ggggtttcga cgctggcgcc gacacacctt tttcccgcgt
     2821 acctcgacga cgtgcagcgt gaggtcgatg agggaccatg cctctcggcg gcctgggagc
     2881 agcacacaat tcgcatcacc gacctcgccg ccgatgaccg gtggccgaag tatcgcgccg
     2941 ccgcgctgaa acgcacgccg gtgcgctcga tcgtctcctt ccagcttttc aacgacgcca
     3001 agagcatcgc cgcgctcaac ctgtatgcgg aaccggcatg cgcattcgac gacgaatcga
     3061 tcgagctggg cctcatctac gctgcgcaca cgaccgtcgc gtggaacgcg atgcgcggtc
     3121 atgagcagtt cagcagtgcc ctggccagcc gcgatctcat cggacaggca aagggcatgc
     3181 tgatggaacg gttcgatatc gacgcgctgg ccgcgttcga gctgctgcgc aagctctcgc
     3241 aagagtcgaa catcaaactc atcgatatcg ccgctaagct cgtgcagggc gagcgaccgg
     3301 ccgagtagcc agggctaggc gccagggccg ttgggccact tgagcatcga acccgcgtcg
     3361 acacgaatgt gttgcccggt gatgtagcgg ctctcctcgc tggcgaggaa cacagccagg
     3421 ttcgcgatgt cctccggctc gacatacgga atgggcatgg cgttgaagta ggtgaaggcg
     3481 ggttcggcat cctcacgggt cggtttcttg ccctcggcca tcatgtcggg ccggaagacg
     3541 ctgtagatgc catcgttctg cagcagcgca gtgttgacgt tggtggggtg catggcgttg
     3601 acccgaatca tcttcggcgc cacctgtaga cagagatggt cgacatagtc gatcagaacc
     3661 cgcttgctcc agccgtagcc cgcgccgccc ggtcccatca tcgggttgtc gctggtgccc
     3721 ttgatcatgc ccgctgtcga acccgtgatg atgaccgagg cgccgttggg caggtggggc
     3781 agggcgaccg cgacggtgtt catcacgccc aacaggtcga cgtcggtggc gtcgacgaaa
     3841 tgcatcgggt cgaactcctt gccgaatgcc atcggcagga tgccggcgtt ggccacgacg
     3901 atgtcgagcc tgcccaggtc ggcgaccccc gcctccaacg cgtcgcgcag ttcgtggcgc
     3961 tcgcggacgt cggcgatcct ggcgtacacg cggcggccga gttccttgac ggaccgctcg
     4021 acgtcggaga ggtcgtccgg agtcgccagg ggatacgggt tcgagtcgat gttccggcag
     4081 atgtcgacga cgatgatgtc ggcaccctcc ttggccatcg caagcgcgtg cgcacgtccc
     4141 tgaccgcggg ctccacccgt gatgaaagcg accttgccct cgagccgtcc agccat
//
LOCUS       DASYLX010001090         7185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1942488,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001090 DASYLX010000000
VERSION     DASYLX010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942488"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGP27_25525"
     CDS             complement(<1..432)
                     /locus_tag="VGP27_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854697.1"
                     /translation="MDFELDDEQRTWVAEVRQFLAMNVTPALKAEMAEHGLEYQGGEL
                     TAFRRKIGEKGWFGLNWPTEYGGLGLTAMHQHLLMSEFEYAGVPGPDLTVTSVAPMIM
                     RHGTEQNKTEFLPGIARGEIVFALGYSEPNAGTDLAGLRTRA"
     gene            complement(488..1447)
                     /locus_tag="VGP27_25530"
     CDS             complement(488..1447)
                     /locus_tag="VGP27_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3533 domain-containing protein"
                     /protein_id="HEV7854698.1"
                     /translation="MRTVPLEIRKVATILAIALFMASSFVWAYTVALGRPIPRHLPIG
                     VVGAPSAQILSELEARAHEFDERRYPSREAAIAAVDRQEVPAVIDAGVTPPQLLVSSA
                     SDPSSARALMQLDQSTPGRYLLPIVDLHPLPASDPAGLATFYLVIAATLLGFVTMFQL
                     RANVKTLTLGRWLAVVGILAVVGGAALAAVAGPILGALRAPFPELWLLVSMQIAIAAL
                     FNSAMIVLIHRWAIIPTWGMFILLGNTSSGGAVSASLLPQPFAFLNHALPSGATVSAI
                     HSAAYFPHNQRLLPYIVLAVWLVLTLTALIIFSRKLGRSPAQA"
     gene            complement(1548..2165)
                     /locus_tag="VGP27_25535"
     CDS             complement(1548..2165)
                     /locus_tag="VGP27_25535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV7854699.1"
                     /translation="MILTPGITGLLVIDPYNDFISEGGKVWDRVKGVAEANGCIPHMK
                     QVLEAAREAQIRVFYALHHRYRLGDYETWKYIAPIQKTAWDRRTFEFGTWGAELRDEF
                     LPQPGDVVAEEHWCSSGFANTDLDLLLKKHGIHELVVVGLIAHTCVEATVRYGAELGY
                     QMTIVKDATTSYSDDHMHAALEVNLPNYAAVVSTDEVVDAVRNSR"
     gene            complement(2162..2815)
                     /locus_tag="VGP27_25540"
     CDS             complement(2162..2815)
                     /locus_tag="VGP27_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HEV7854700.1"
                     /translation="MKNAQLSRLVRGVASAPLSFAWLAVLFVTTRIQRSAGRRKAKRI
                     QRTHSTNLRRLRAEPSRVLAASLFWLDDRKWSPYVPMFVGVVAPAERRLRWWRWFLVG
                     LGAHVIGTYVGQSYLRLQIRRGRAPKRLLDARDVGVSYFLLGVAGTVTGYVAPPWRSR
                     SQVTAVAALSVNAAARPTFTEIGHLTALLVGLAAIPLAPDRDRLPYPPAASRSKMGG"
     gene            3097..4383
                     /locus_tag="VGP27_25545"
     CDS             3097..4383
                     /locus_tag="VGP27_25545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7854701.1"
                     /translation="MNTDTTFADLGVSQPLINVLTERGITQPFPIQTETLPDTLAGRD
                     VLGRGKTGSGKTLAFAIPLVTRLAGRRGRASRPSGLVLAPTRELATQISAALQPLAAA
                     CGLTVTTIFGGVTQSRQVTALKAGADIVVACPGRLEDLMRQRLVSLDSVEITVIDEAD
                     HMADLGFLPGVTRILAATPAGGQRLLFSATLDNGVDKLVKRFLRNEVMHSVDSADSHV
                     PAMTHHVFHVSDAQAKKELVHRLASGHGRRILFMRTKHQARKLAKQLTESGVPSVDLH
                     GNLSQPARDRNLAAFAAGEARVLVATDIAARGVHVDGIELVVHIDPPAEHKAYLHRSG
                     RTARAGNDGDVVTVVLPEQRRDTQALMRRAGIKVTPQHVTADSAAVHAVAGEVAPYRA
                     PKPTSAPPAAPPSSSQRPKNGGRRGGRPSRARAGAR"
     gene            4460..5314
                     /gene="ppk2"
                     /locus_tag="VGP27_25550"
     CDS             4460..5314
                     /gene="ppk2"
                     /locus_tag="VGP27_25550"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEV7854702.1"
                     /translation="MSTDEHGYTIETEDDDDPVLLDRDGRPVETWRERYPYDERMSRN
                     EYEWLKRRLQIELLKLQNWSKRTGARHVILFEGRDAAGKGGTIKRLMEHLNPRGARVV
                     ALEKPTDTEKSQWYFQRYAAHLPSSGEMVLFDRSWYNRAGVERVMGFCTPEQYLEFLR
                     QAPMFEDMLVSEGIHLTKFWFSVSPAEQRTRFAIRLVDPVRQWKFSPMDLASIDKWSE
                     YTAAKEDMFRVTDTDIAPWIVVKSNDKKRARINTMRFLLAQFDYDDKDESLVGEPDPL
                     IVGRALED"
     gene            complement(5331..7151)
                     /locus_tag="VGP27_25555"
     CDS             complement(5331..7151)
                     /locus_tag="VGP27_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7854703.1"
                     /translation="MDTAPFGHYQLQELIGRGGMGEVYRAFDTKTDRIVALKLLPSHL
                     AKDAVFQQRFRRESQAAAGINEPHVVPIHGFGEIDDKLYLDMRLIDGRNLGAMLGEDD
                     DKTPLKPALAVSITDQVAAALDAAHELGLIHRDVKPSNILVTKNDFAYLIDFGLARTA
                     GQQGLTTAGSTLGTMAYMSPERFEGGEIDSRSDVYALSCVLYECLTGSRPYPVDSLEQ
                     QIAMHINAPAPKPSAVDPKLAAFDEVIARGMAKKPSRRYQTAGEMAAAARRALAVPVR
                     TTGRSGRHSARRTKASTPGRGLSGRVLAILGAAVLLGAALVFGAWHFWGGSEGAPSAG
                     PTATSAPPGPSDDAGGLVPAIAATLPAGIKESGRLTVGVNVPYAPNEFKNSAGDIVGF
                     DVDLINAVAKTLGLIPEYRESLFEDIIPAVQSSEINVGMSSFTDTLERQEQVDFVTYL
                     EAGTLWAQRSGSGVDPNAACGLRIGMTPGSTHETVEIPAKRDQCVAAGLPPVQAVVYD
                     RQDALTAALINGQIDAMSADSPVTGFAIKGSGGALEPAGEQFDTAPYGWPVQKDSGLA
                     ESLRQALEHLMSTGQYEAIATKWGVDKGMIAKPVINGAIS"
BASE COUNT         1233 a         2470 c         2303 g         1179 t
ORIGIN      
        1 cgcgcgggtc cgcaggccgg ccaggtcggt gcctgcgttg ggctcggagt agccgagcgc
       61 gaagacgatc tcccccctgg cgatgccggg caggaattcc gtcttgttct gctcggtgcc
      121 gtgccgcatg atcatcggcg ccaccgacgt caccgtcagg tcgggcccgg gcacaccggc
      181 gtactcgaac tcgctcatca gcagatgctg atgcatcgcc gtgaggccga ggccgccgta
      241 ctccgtcggc cagttcaggc cgaaccagcc cttctcgccg attttgcggc ggaacgccgt
      301 cagctccccg ccctggtact ccagcccgtg ctcggccatc tcggccttca gcgcgggcgt
      361 gacgttcatg gccaggaact gccgtacctc ggcgacccat gtgcgctgct cgtcgtcgag
      421 ctcgaagtcc acgcaggcga cactatcgag aaggtgtcta ccggtgcggg cgaacgggac
      481 aacgccgtca tgcttgcgcc ggcgaacggc ccagcttgcg cgagaagatg atcaacgcgg
      541 tcagcgtgag gacgagccac accgccaaga cgatgtacgg aagcagccgc tgattgtgcg
      601 ggaagtacgc cgccgaatgg atcgcgctca ccgtcgcccc gctcggcagg gcgtggttca
      661 ggaacgcaaa cggctgcggc agaagcgatg cggacaccgc gccgcccgac gaggtgttgc
      721 caagcagaat gaacatcccc cacgtcggga tgatcgccca ccgatgaatc agcacgatca
      781 tcgccgagtt gaacaacgcc gcgatcgcga tctgcatcga caccagcagc cacagctccg
      841 ggaacggcgc acgcaacgcc ccgaggatcg gcccggccac cgcggcgagc gccgcgccac
      901 cgacgaccgc caggatgccg actaccgcca gccatcgccc tagcgtcagc gtcttgacgt
      961 tggcgcgcag ctggaacatc gtcacgaacc cgagcagcgt ggccgcgatg acgagataga
     1021 acgtcgccag ccccgcggga tccgacgccg gcagcggatg caggtcgacg atcggcaaca
     1081 gataccgccc cggcgtggac tggtcgagtt gcatcaaggc gcgcgccgac gacgggtcgc
     1141 tcgcgctcga caccagcagt tggggcggcg taacgccggc gtcgatgacc gccggcacct
     1201 cctggcgatc gaccgccgcg atcgccgcct cacgcgacgg gtatctgcgc tcgtcgaact
     1261 cgtgggccct ggcctcgagc tcactgagga tctgcgcgga cggcgcgccg acgacaccga
     1321 tcggcaggtg ccggggaatc ggtctgccca gcgcaacggt gtaggcccag acgaacgacg
     1381 acgccatgaa cagtgcgata gcgagaatcg ttgcgacctt gcggatctcg agcggtaccg
     1441 tcctcactcg ctccatcatt gacatggaca tggctgtgat ccggactacc ccggcagccc
     1501 tgcaggaaac atcgacgttt cggtcaacgg tcagcggcgc gcccgactca tcggctgttg
     1561 cgaaccgcgt cgacgacctc gtcggtggac accacggcgg cgtagttggg gaggttcacc
     1621 tccagcgctg cgtgcatgtg gtcgtcggag tagctggtcg tggcgtcctt cacgatggtc
     1681 atctggtagc cgagttcggc gccgtagcgc acggtcgcct cgacgcaggt gtgcgcgatc
     1741 aaaccgacga cgacgagctc gtgtatgccg tgcttcttga gcagcaggtc caggtcggtg
     1801 ttggcgaaac cgctcgagca ccaatgctcc tcggccacga cgtcgcccgg ctgcggcaga
     1861 aactcatcgc gcagctcggc gccccacgtg ccgaactcga acgtcctgcg gtcccacgcg
     1921 gtcttctgga tgggcgcgat gtacttccac gtctcgtagt ctccgaggcg gtaccggtgg
     1981 tgcagcgcgt agaacacccg gatctgcgct tcgcgcgcgg cctcgagcac ctgcttcatg
     2041 tgcgggatgc agccgttggc ctcggccacg cccttgacgc ggtcccagac cttgccgcct
     2101 tcggagatga agtcgttgta cgggtcgatt acgagcagac cggtgatgcc cggcgtcaag
     2161 atcatccgcc catcttgctt cgggatgcgg ccggtgggta cggcaatcgg tcacgatcgg
     2221 gcgccaacgg gatggcggcg agaccgacca gcaacgccgt gagatgcccg atctcggtga
     2281 aggtcggcct ggcggctgcg ttgacgctca gcgcggcgac ggctgtgact tggctgcggg
     2341 atcgccacgg cggtgcgacg tagccggtca cggttcctgc cacgcccaag aggaagtagc
     2401 tgactccgac atcgcgagca tccaggagtc gcttcggcgc tctgcccctg cgaatctgca
     2461 gacgcaggta gctctggccg acgtacgttc caatgacgtg agcgcccaaa ccgacgagaa
     2521 accaacgcca ccaccgcaac cgccgttcgg cgggcgcgac gacgccgacg aacatcggaa
     2581 cgtaaggcga ccatttgcgg tcgtcgagcc agaacaggct tgcggccagc acgcgcgacg
     2641 gctccgctcg caggcggcgc agattggtgg agtgggtccg ctggattcgt ttggccttgc
     2701 gtcggccggc cgaccgctga atgcgtgttg tgacgaacag cacggccagc caggcgaagc
     2761 tgagcggagc actggccacg ccacgaacca gccggctcag ctgcgcgttc ttcatcacat
     2821 tgaacgtagt cgtgcgggat tccgcggcat cgtgggctat catgaataca acgttggctg
     2881 ttcaatgcaa tcagctgaca cgtcgtgaaa cgcttcttca tccctccggc gccgcccaga
     2941 aattgggccg tgcgaccgcc tcagatgatt cgtgcaccca gcacagctgt atcgcggcga
     3001 tcgattttgc ccaccggcaa tctcgccacc atgtgctgag ctaccgcgcg accatgcgcc
     3061 gccaattcat cacatcgatg cctgaaaggc accgccctga ataccgacac gactttcgcc
     3121 gacctcggcg tcagccagcc cttgatcaac gtgctcaccg agcgcgggat cactcaaccc
     3181 tttcccatcc agaccgaaac cctgccagac acccttgccg gccgcgacgt gctcggccga
     3241 ggcaagaccg gcagcggcaa gacgctggcc ttcgccattc cgctggtcac caggctcgcc
     3301 gggcgacgcg gccgcgcgtc gcggccgtcc ggacttgtgc tcgctccgac ccgcgaactg
     3361 gccacccaga tcagcgccgc actgcagcca ctggccgccg cctgcggcct gacggtgacc
     3421 acgatcttcg gcggcgtgac ccagtcccga caggtcaccg cgctcaaggc tggggccgac
     3481 atcgtcgtcg cctgcccggg ccggctcgaa gatctgatga ggcaacgact cgtcagcctt
     3541 gattcggtcg agatcaccgt catcgacgag gccgatcaca tggccgatct cggcttcctg
     3601 cccggtgtga cgcgcatcct cgccgccacc cccgcgggcg gacagcggct gctgttctcc
     3661 gcgacgctcg acaacggcgt cgacaaactg gtcaagcgct tcctgcgcaa cgaggtcatg
     3721 cactccgtcg acagcgccga ctcgcatgtt cccgcgatga cccaccacgt ctttcatgtc
     3781 tccgatgcgc aggccaaaaa ggaactggtg caccgcctcg cctccgggca cggccgccgg
     3841 atcctgttca tgcgcaccaa gcaccaggcc cgcaagctcg cgaaacagct gactgaatcg
     3901 ggtgttccgt cggtcgatct gcacggaaac ctttctcagc cggcgcggga ccgcaacctt
     3961 gcggcgttcg ccgcaggcga ggcccgcgtg ctggtggcga ccgatatcgc cgcgcgtggt
     4021 gtgcacgtcg acggcatcga actcgtggtt cacatcgatc cgcccgccga gcacaaggcc
     4081 tatcttcacc gctccggacg caccgctcgg gccggcaatg acggcgacgt cgtcaccgtc
     4141 gtgctacccg aacagcgcag ggacacacag gctttgatgc gccgtgcggg aatcaaggtg
     4201 acaccgcagc acgtgaccgc cgactcggcc gccgtgcacg ccgtcgcagg cgaggtcgcg
     4261 ccctaccggg ctccgaagcc gacgtcggct ccgccggcgg cgccgccgtc gtcctcgcaa
     4321 cgaccgaaga atggtgggcg acggggtggt cgtccctcgc gcgcgagggc gggtgctcgc
     4381 tagcgccgct cgctgattgg gtatcccacc tttcgtgggt gccaccaatg acgacgacct
     4441 cgacatcaat ctcgaagact tgtcgaccga cgagcacggc tacacgatcg agaccgagga
     4501 cgacgacgat ccggtgctgc tcgaccgcga cggcaggccc gtcgagacct ggcgcgagcg
     4561 atacccctac gacgagcgga tgtcgcgcaa cgaatacgag tggctgaagc gccggctgca
     4621 gatcgagctt ctgaagctgc agaactggag taagcggacc ggcgcgcggc acgtcatcct
     4681 cttcgagggt cgcgacgccg ccggtaaggg cggcaccatc aagcggttga tggagcacct
     4741 caacccgcgc ggcgcgcgcg tcgtggcgtt ggagaagccg accgacaccg agaagtcgca
     4801 gtggtatttc cagcgctacg cggcgcacct gccgtcgtcg ggcgagatgg tcctgttcga
     4861 tcgatcctgg tacaaccgcg ccggagtgga acgggtgatg ggcttctgca cgcccgagca
     4921 gtatctggag ttcttgcggc aggcgccgat gttcgaggac atgctcgtca gcgagggcat
     4981 tcacctgacc aagttctggt tctcggtgtc gccggctgag cagcgcaccc ggttcgcgat
     5041 ccggctggtg gacccggttc ggcagtggaa gttctcgccg atggacctgg cctcgatcga
     5101 caagtggtcg gagtacacgg ctgccaagga ggacatgttc cgcgtgacgg acaccgacat
     5161 cgcgccgtgg atcgtggtga agagcaacga caagaagcgc gcccgcatca acacgatgcg
     5221 cttcctgctc gcccagttcg actatgacga caaggacgag tcattggtcg gcgagcccga
     5281 tccgctgatc gtcggccggg cgctcgagga ctaggtcggc ggcggggcga tcagctgatc
     5341 gcgccgttga tcaccggctt ggcgatcatg cctttgtcca ctccccattt ggtggcgatg
     5401 gcctcgtact ggccggtcga catgaggtgc tcgagcgcct gccgcaggga ttcggccaac
     5461 cccgaatcct tctgcaccgg ccatccgtac ggcgcggtgt cgaactgctc gcccgcgggt
     5521 tccagtgcgc cgccgctgcc cttgatcgcg aaccccgtca ccggtgagtc cgccgacatc
     5581 gcgtcgatct gaccgttgat caacgcggcg gtgagcgcgt cctggcggtc gtagacgacg
     5641 gcctgcaccg gcggcagtcc ggccgcgacg cattgatcgc gtttggccgg aatctcgacg
     5701 gtttcatgag ttgagccagg cgtcatccca atccgcaacc cgcacgccgc atttgggtcg
     5761 acgcccgatc cgctgcgctg cgcccacaac gtccctgctt ccaaataggt gacgaagtcg
     5821 acctgctcct ggcgctccag cgtgtcggtg aacgacgaca tcccgacgtt gatctctgaa
     5881 ctctgaaccg ccggaatgat gtcttcgaag agggactccc gatactccgg gatgaggccg
     5941 agcgtcttcg ccaccgcatt gatcaggtcg acgtcgaagc cgacgatgtc acccgcggag
     6001 ttcttgaatt cgtttggggc gtagggcacg ttgacgccga ccgtcagccg gcccgactcc
     6061 ttgatgcctg cgggcagcgt cgccgcgatc gccggcacca acccgcccgc atcgtccgag
     6121 ggccccgggg gcgccgacgt cgccgtcggg cctgccgaag gggcaccctc gcttccgccc
     6181 cagaagtgcc acgcgccgaa aaccagtgcc gcaccgagca gcaccgcggc accaaggatc
     6241 gccaggacgc gcccggacaa tccccgcccc ggcgtcgatg ccttggtgcg ccgagccgaa
     6301 tgccgcccgg atctaccagt cgtccgcacg ggcacggcga gtgcgcgtcg cgcggcggcg
     6361 gccatttcac ccgctgtctg atagcgccgg ctcggcttct tggccatccc cctggcgatc
     6421 acctcgtcga acgcggccag cttcggatcg accgccgacg gcttcggcgc aggcgcgttg
     6481 atgtgcatgg cgatctgctg ctcgaggctg tcaacgggat acggccgcga acccgtcagg
     6541 cattcgtaga gcacgcagga cagcgcatag acgtccgagc gcgaatcgat ctcgccgccc
     6601 tcgaaccgct cgggcgacat gtaggccatg gtgcccagcg tgctgcccgc cgtcgtcagc
     6661 ccttgctgac cagccgtgcg ggccaacccg aagtcgatga ggtaggcgaa atcgttcttg
     6721 gtgaccagga tgttcgacgg cttcacgtcc cggtggatca aacccaattc gtgcgcggcg
     6781 tccagcgctg ccgcgacctg gtcggtgatc gacaccgcca gcgccggctt cagcggcgtc
     6841 ttgtcgtcgt cctcgcccag catcgccccg aggttgcggc cgtcgatcag ccgcatgtcg
     6901 aggtagagct tgtcgtcgat ctcgccgaag ccgtggatcg gcacgacgtg cggttcgttg
     6961 atgcccgctg ccgcctgcga ctcccgccgg aagcgctgct ggaagacggc atcctttgcc
     7021 aggtgcgacg gcagcagctt cagcgcgacg atgcgatcgg tcttggtgtc gaaggccctg
     7081 tagacctcgc ccattccgcc ccgaccgatc agctcctgca gctgatagtg cccgaatggc
     7141 gccgtatcca ccgcgtttac tcctcggccg gcgaacccgc tttga
//
LOCUS       DASYLX010001091         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2821056,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001091 DASYLX010000000
VERSION     DASYLX010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821056"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VGP27_25560"
     CDS             complement(<1..1184)
                     /locus_tag="VGP27_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7854704.1"
                     /translation="MTNDPRYSPPPPHHGRRPGDDRTAVYPSGQPGVPPARGGGHTGY
                     QQQPYDWRYATQQQQHAYRQPYDPYRGAPQQPPASGRYPQVGQPPRPPQKRSRAGALT
                     VGALTIAVVSAGIGGGVALMAKPVGYSGGGTQITAAPSVPAASLPAGSVEQVAAKVVP
                     SVVKLETDMGRASEEGSGIILSSDGLILTNNHVVEGAKGGDTTITFADGRTTKFTVVG
                     TDPSSDIAVVKADGVSDLTPITIGSSANLKVGQDVVAVGSPLGLEGTVTTGIISALNR
                     PVAAGGDIKNQNTVLDAIQTDAAINPGNSGGALVNMNGELVGVNSAIATLGGDSADAQ
                     SGSIGLGFAIPIDQAKRIADELIKGGSASHASLGVQVGNDASSHGAKIVEVTGGGAAA
                     AAG"
     gene            complement(1332..>1557)
                     /locus_tag="VGP27_25565"
     CDS             complement(1332..>1557)
                     /locus_tag="VGP27_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7854705.1"
                     /translation="FFRSTSARAMPGSGLGLAIVKQVVHKHGGALRVDDTVRGGQPPG
                     TSIYVLLPGRVIPATRPAEVAQPMASGDNG"
BASE COUNT          216 a          508 c          542 g          291 t
ORIGIN      
        1 ccggccgccg cggctgcacc accaccggtg acctcgacga tcttggcgcc gtgtgacgag
       61 gcgtcgttac cgacctggac gcccagcgat gcgtgcgagg cgctgccgcc cttgatcagt
      121 tcgtcggcga tccgcttggc ctgatcgatc ggaattgcga agcccaaacc gatcgagccg
      181 ctctgcgcgt cggcggagtc accgccgagg gtcgcgatgg ccgaattcac gcctaccagt
      241 tcgccgttca tgttgaccag cgccccgccc gagttaccgg ggttgatcgc ggcgtcggtc
      301 tggatcgcgt cgagcacggt gttctggttc ttgatatctc cgcccgcggc gacgggccgg
      361 ttcagcgcgc tgatgatgcc ggtcgtcacg gttccttcga ggccgagtgg cgaaccgacc
      421 gccaccacgt cctggccgac cttgaggttg gccgacgagc cgatggtgat cggcgtgagg
      481 tcggagacac cgtcggcctt gacgaccgcg atgtcgctgc tggggtcggt gccgaccacg
      541 gtgaacttgg tggtccggcc gtcggcgaag gtgatcgtgg tgtcgccgcc cttggcgcct
      601 tcgacgacgt ggttgttggt gaggatcagg ccgtcggagg acaggatgat gccggagccc
      661 tcttcggatg cccgccccat gtcggtctcc aacttcacga cgctgggcac caccttggcc
      721 gcgacctgct cgacggatcc ggcgggcagg ctggctgcgg gcacgctggg cgccgcggtg
      781 atctgggttc cgccgccgct gtaaccgacg ggtttcgcca tcagggcgac gccgccgccg
      841 atgcctgccg acaccacggc gatggtcagc gcaccgacgg tcaaggctcc tgcgcgcgat
      901 cgcttctgcg gcggccgggg aggttgtccg acctgcgggt acctcccgct tgcgggcggc
      961 tgctgcgggg cccctcgata ggggtcatag ggctgccggt aggcgtgctg ttgctgctgg
     1021 gtcgcatatc gccagtcgta gggctgctgc tgatagccgg tatgccctcc cccgcgagcg
     1081 ggtggtaccc ccggctgacc agagggatat accgccgtgc gatcgtcgcc gggcctgcgg
     1141 ccgtgatgcg ggggcggcgg tgaatacctc gggtcgttcg tcatgtcgct acgggctctt
     1201 tctacgagtt cggcacattc cgacacttct acaacgaagt tccgcgcctc tacggtgccg
     1261 gggtgggctg agaatccact tagagaatgt tcggcacatt ccgagacttc cggctggtgg
     1321 ttcccgaccg tttacccatt gtcgccgctg gccatcggtt gtgcgacctc cgcaggacgt
     1381 gtcgcgggaa tcacccgtcc gggcagcaac acatagatag aggtgcccgg cggctggcct
     1441 ccgcgcacgg tgtcgtccac ccgaagcgcg ccaccgtgtt tgtgtacgac ttgtttgacg
     1501 atcgccagac caaggcccga gccgggcatg gcccgcgcgg aggtggaccg gaagaac
//
LOCUS       DASYLX010001092         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3589303,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001092 DASYLX010000000
VERSION     DASYLX010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3589303"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGP27_25570"
     CDS             complement(<1..120)
                     /locus_tag="VGP27_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854706.1"
                     /translation="METFEGRAAVITGGASGIGFACAQEFTRRGARVVLADIDQ"
     gene            complement(195..1210)
                     /locus_tag="VGP27_25575"
                     /pseudo
     CDS             complement(195..1210)
                     /locus_tag="VGP27_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263897.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(1264..2103)
                     /locus_tag="VGP27_25580"
     CDS             complement(1264..2103)
                     /locus_tag="VGP27_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854707.1"
                     /translation="MTGRVEGKVAFITGAARGQGRSHAVRLAEEGADIIAIDICKPVV
                     AEGGIPPATPEDLAETADLVKGHNRQIYTAEVDVRDYDALKAAVDAGVDQLGRLDIIV
                     ANAGIGNGGETLDKTSEEDWTDMIDINLSGVWKTVKAGVPHLIEGGRGGSIILTSSVG
                     GMKAYPHCGHYVAAKHGVVGLMRTFAVELGHHMIRANSVHPTHVSTPMIMNEGTWKMF
                     RPDLENPGADDMAPICQMFHTLPIPWVDPIDVSNAVLFFASEEARYLTGVTLPIDAGS
                     CLK"
     gene            complement(2139..>2967)
                     /locus_tag="VGP27_25585"
     CDS             complement(2139..>2967)
                     /locus_tag="VGP27_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854708.1"
                     /translation="IGADRFDFVADLGAQMPMKVISALLGIPEGDQEMIRDHANAQLR
                     TEAGKPMKHEDGGAMVLGELFETYIDWRAEHPSDDIMTELLNAEFVDETGSTRNLTRQ
                     ELLTYLNVVAGAGNETTTRLIGWAGKVLAEHPGQRRELAENPTLIPQAIEELLRFEPP
                     APHVARYVTRDVEIHDQTVPEGSVMMMLIGAAVRDHRQFPPDGDVFDIHREARQHLSF
                     SVGTHYCLGSALARLEGRVALEEILKRFPDWEVDMANATLSPTSTVRGWDSMPAVLK"
BASE COUNT          479 a          984 c          969 g          535 t
ORIGIN      
        1 ctgatcgatg tcggccagca cgacgcgcgc gccgcggcgg gtgaactcct gcgcgcaggc
       61 aaaaccgatg ccgctcgcac cgccggtgat taccgctgcg cgcccctcga atgtctccac
      121 gccgcgaaca ctatccggcg aggcgaattg ccgggctcct caacgcggcg ccacgccgct
      181 tgatcgtcgc ccggttaggc gatggcgtca atcgccgcac ggctgtcgag gtcgacgaag
      241 gccgccgcat accgctgcac caactccagg cagacggccg ggctctgcca ggcatcaccg
      301 ccggacagcg tcgccatcca gatcgccagc ccgtgggcga ccgaagcgcg ctagcgcagc
      361 agcacctcgt cggggctcgg catttcgtcg gcgggcaacg tcagcgcagc gcggtactga
      421 tcgagcagct cgcgctcgct gcggcgcctt tcgtcgatgg tcagcgcgcc ctgcaggaag
      481 tacccgaggt ccagggacca gttgccgcgg cgtgccatct gccagtcgag gaacccgaca
      541 tcgtcatcgg gcagcacatg ggtgttgccg atgtgcgggt caccgtgcag cagcgtctgc
      601 ggtgatgtcg tcagcgtgcc gatgtagcgg gcccagatat cgatgaacaa ctgctcgccg
      661 gtcagcgcca ggatctcggc gggcacggta tcgcccgacc gctcacgcgc gatgtccagc
      721 ggcgcgtact gcattccgtc gaaagcaacg aagggctcga cccagccgag cgccgggttg
      781 tccgtcagcc gggcacccca atacgcgctg tgcagtcggg caagcccacg caccccgttg
      841 gccacttgct cgaccgtcat cggccgggtg gagtcacgcg tgggtccgca ccgcgcgcga
      901 cgacgtcctc catgatcatc aggaagtcgg agtgctcttc gtcgatgagc gccgcgtaga
      961 cggtcggatg atcaagcggc agaggcactc cctacgagaa gagccggggc tcatggtaga
     1021 ggccgctggt cagcgccacg agttcggcat gcgcggggtc gaccgccttg gcgaacacgg
     1081 tggcgggtcc ggagtcggcc gagtacgtca atgccagtcg ggcccgcttg ttcgtgccgt
     1141 cgtcgcgcaa cgagacccga acgtcatcca ccacggcgcc gggatggtgt tcggcgagcg
     1201 ccgccgtcat ccactccggg gtgatcgcat cccagccctg tgggatcgac agcctcgcaa
     1261 tcgtcacttc aggcagctac ctgcgtcaat cgggagcgtc acgccggtca ggtagcgagc
     1321 ctcctcggat gcgaagaaca gcaccgcgtt gctgacgtcg atcgggtcca cccacgggat
     1381 gggcagcgta tggaacatct ggcagatcgg ggccatgtcg tcggcgccgg ggttctccag
     1441 atcgggccgg aacatcttcc acgtgccctc gttcatgatc atcggggtgc tcacatgcgt
     1501 ggggtgcacc gaattggcac gaatcatgtg gtgacccaac tccaccgcga acgtccgcat
     1561 gagaccgacg acgccgtgtt tggcggccac gtagtgaccg cagtgcgggt aggccttcat
     1621 accgccgacc gagctggtca gaatgatcga gccgccgcgt ccaccctcga tgaggtgcgg
     1681 cacaccggct ttcacggtct tccacacacc gctcaggttg atgtcgatca tgtccgtcca
     1741 gtcttcttcg ctggtcttgt ccagcgtctc gccgccgttg ccgatgcctg cgttggccac
     1801 gatgatgtcg aggcggccca gctgatcgac gcctgcgtcg accgctgcct tgagcgcgtc
     1861 gtaatcgcgc acgtccacct cggccgtgta tatctgtcgg ttgtggccct tgaccagatc
     1921 tgcggtctcg gccaggtcct ccggtgtcgc cggcgggatc ccgccttctg caaccactgg
     1981 tttgcagatg tcgatcgcga tgatgtcggc gccctcctcg gctagccgaa cagcgtggct
     2041 gcggccttga ccacgcgcgg ccccggtgat gaaagcgacc ttgccttcaa cacgtcctgt
     2101 catattccaa cctcaattcg tggttatggg aaaggatttc acttgagaac agccggcatg
     2161 gagtcccagc cgcggacagt cgatgtaggg gagagcgttg cgttggccat gtcgacttcc
     2221 cagtcaggga accgcttgag gatctcctcg agtgcgacgc gtccctcaag gcgggccagc
     2281 gccgagccga ggcagtagtg cgttccgacg ctgaaggaaa ggtgctgacg tgcttcgcga
     2341 tggatgtcga aaacatcccc gtcgggcggg aactgacgat gatcgcggac cgccgcgccg
     2401 atcagcatca tcatcacgct gccttcgggc accgtctggt cgtggatctc gacgtcgcgg
     2461 gttacgtagc gcgcgacgtg cggcgcgggc ggttcgaacc gcagcagctc ctcgatcgcc
     2521 tgtgggatca gcgtcgggtt ctcggcgagt tcgcggcgct ggccggggtg ctcggccaaa
     2581 acctttcccg cccagccgat cagccgcgtg gtggtctcgt tgcccgcgcc cgccaccacg
     2641 ttgaggtacg tcagtagttc ctggcgcgtc aggttacgag tggagccggt ctcgtcgacg
     2701 aactccgcgt tgaggagttc ggtcatgatg tcgtccgacg gatgctcggc gcgccagtcg
     2761 atgtaggtct cgaacagctc gccgagcacc atggcgccgc cgtcctcgtg tttcatcggc
     2821 ttgccggcct cggtgcgcaa ctgcgcgttg gcgtggtcgc ggatcatctc ctggtcaccc
     2881 tcggggattc ccagcaatgc gctgatcacc ttcatcggca tctgggcgcc caggtcggcg
     2941 acgaagtcga agcggtccgc cccgatc
//
LOCUS       DASYLX010001093         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1395585,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001093 DASYLX010000000
VERSION     DASYLX010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395585"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VGP27_25590"
     CDS             complement(<1..627)
                     /locus_tag="VGP27_25590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7854709.1"
                     /translation="MLGDCLTLIDSLPFFTECKRESYDLLAARPGSIILEVGCGLGDD
                     AAALARRVAPGGSVVAVDGSQAVVDAARRRHSDIAGLSFDVADATQLPFVDETFDGCR
                     IDRVLQHVPDPAAAIREMARVLRPGGVLVAFDNDWETLTVDSADRAMTRTVLNAWCDR
                     FPSGWIGRRMVALFLEAGLEDVETHPKTLVCSDLDVADRVFSFFATADG"
     gene            complement(757..1314)
                     /locus_tag="VGP27_25595"
     CDS             complement(757..1314)
                     /locus_tag="VGP27_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7854710.1"
                     /translation="MASRTRNPAATKAAILAAARLQFGQSGFEKTTIRSVATAAGVDP
                     ALVMHYFGNKDGLFGEVSRLDISPPDLAGIAPGDVADVMLPLFVEVWGPDGPFLPLLR
                     AAASSQAAADALLDVFARQVAPALADVAIDRPAERAALIGAHLLGIAVARYIIGIPPL
                     VDMDDKTLACWLKPVLTHYLTDPAP"
     gene            1401..1814
                     /locus_tag="VGP27_25600"
     CDS             1401..1814
                     /locus_tag="VGP27_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854711.1"
                     /translation="MTVTAIQIGLHPDAVDYESADFSQFKGLSREGLSRANTDNVAGL
                     RSAGYEVDNCLIGTGEAGAEKARAWLSAKRYDAVLIGAGVRLVAANTLMFEAIVNAAH
                     AANPGCRFVFNYSATSTPDDIRRWYPNPANVTPAV"
     gene            1817..>2407
                     /locus_tag="VGP27_25605"
     CDS             1817..>2407
                     /locus_tag="VGP27_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854712.1"
                     /translation="MDASGVDVLVVGAGPAGLTAAGDLARRGRSVAVLERRPKMYPAS
                     RAFATMARTLEVLDARGLADGVLAQAHRAPGVAIFAGARIDLTHLRSAYPFVAITPQT
                     NVDRALAEYAAMQGAEIRRGFEVVALEQDADGVTVTARPKDDDDPQHRQTWRAKYVIG
                     ADGAHSTVRDLVGAEFPGKTILSSVVLADVKLAEGPT"
BASE COUNT          425 a          809 c          805 g          368 t
ORIGIN      
        1 gccgtcggcg gtcgcgaaga agctgaacac ccggtcggcg acgtcgaggt cgctgcatac
       61 cagtgtcttc ggatgggtct cgacgtcctc gaggcctgct tcgaggaaca gcgcgaccat
      121 ccggcggccg atccatcccg acgggaatcg gtcgcaccag gcattcagga ctgtgcgcgt
      181 catcgccctg tccgccgagt cgaccgtcag cgtctcccag tcgttgtcga acgcgaccag
      241 gacaccgccg gggcgcaaca cccgagccat ttcgcggatc gccgctgcgg gatcgggtac
      301 atgctgcagc acccggtcga tgcggcagcc gtcgaacgtc tcatcgacga agggcaactg
      361 cgtggcgtcg gcgacgtcga acgacaggcc cgcgatgtcg ctgtggcgcc tgcgggccgc
      421 gtcgaccacc gcctggctgc cgtcgacagc gaccaccgaa ccgccgggcg cgacgcgcct
      481 cgccagcgcc gccgcgtcgt ccccgagacc gcacccgacc tccaggatga tgctgcccgg
      541 ccgggcggcg agcaggtcat agctttcgcg cttgcactcc gtgaagaacg gcagcgagtc
      601 gatcagcgtc aggcaatccc ctagcaagct gtgggccagg cgaacacctc gaaatccgaa
      661 gcgcgatcca cagtctggaa tcccgcggcg agatcatcgg ccatcgttgt cccctaacct
      721 gtcccgagca gaagctccac cacgcaacga taaggcttag ggtgccggat cggtgagata
      781 gtgcgtgaga acgggtttga gccagcaggc gagcgtcttg tcgtccatgt cgacaagcgg
      841 tggaattccg atgatgtagc gggcgaccgc gattccgagc aggtgggcac cgatgagagc
      901 agcccgttcg gcaggccggt cgatcgccac gtcggccagc gcgggcgcga cctgccgggc
      961 gaacacgtcg agcagcgcgt cggccgcagc ttggctcgac gccgccgccc gcagcagcgg
     1021 aagaaacgga ccgtcgggtc cccacacctc gacaaacagc ggcagcatga cgtcggcgac
     1081 gtcccccggt gcgattccgg ccaggtccgg aggcgagatg tcgaggcgcg acacctcacc
     1141 gaagaggccg tccttgttgc cgaagtagtg catcaccaag gccggatcga cacccgccgc
     1201 ggtcgcgacc gaccggatcg tcgtcttctc gaatccgctc tggccgaact gcaacctcgc
     1261 cgcggccagg atggcggcct tggtggcggc tgggttccgt gtcctcgaag ccatgccgcc
     1321 agtgtattca acagttgttg acaagtctgc atccggagcc taacgtcagt tcaacaagcg
     1381 ttgaactgaa ggagtgcgac atgactgtca ccgctatcca gattgggctg caccccgacg
     1441 ccgtcgacta cgagtcggcc gacttctccc agttcaaggg gctgtcccgg gagggactga
     1501 gcagggccaa caccgataac gtcgccgggt tgcgctcggc aggctacgag gtggacaact
     1561 gcctgatcgg caccggcgag gcgggtgccg aaaaggcccg cgcatggctg tccgcgaagc
     1621 gctacgacgc cgtcctcatc ggcgcaggcg tccgactcgt cgccgccaac accctgatgt
     1681 tcgaggcgat cgtgaacgcg gcgcacgccg ccaacccggg atgccggttc gtgttcaact
     1741 actcggcgac ctcgacaccc gacgacatcc gccggtggta ccccaacccg gccaacgtga
     1801 ctccggcggt gtgatcgtgg atgcctccgg cgtggacgtc ctcgtcgtcg gtgccggccc
     1861 ggccgggttg accgcggccg gtgacctggc ccggcgcggt cggtcggtgg cagtgctcga
     1921 acgccggccg aagatgtacc cggctagccg cgcattcgcc acgatggcca ggaccctgga
     1981 ggtgctcgac gcccgcgggt tggccgatgg cgttctcgca caagcacacc gggcgcccgg
     2041 tgttgccatc ttcgccgggg cgcgaataga tctgacgcat ctgcgttcgg catacccgtt
     2101 cgttgcgatc accccgcaga ccaacgtcga cagagcgctg gcggaatacg ccgcgatgca
     2161 gggagcggaa atccgtcgcg gcttcgaagt cgttgcgctc gaacaggatg ccgatggcgt
     2221 cacggtaacg gcgcgaccga aggacgacga cgatccgcag caccgccaga catggcgtgc
     2281 gaaatacgtg atcggcgccg atggcgcaca cagcactgtg cgggatctcg tcggcgcgga
     2341 gtttccgggt aagacgatcc tgtcgtcggt ggtgctggcc gacgtgaagc tggccgaggg
     2401 gccgacc
//
LOCUS       DASYLX010001094         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3918900,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001094 DASYLX010000000
VERSION     DASYLX010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3918900"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGP27_25610"
     CDS             <1..675
                     /locus_tag="VGP27_25610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7854713.1"
                     /translation="GVSFAVREGEILGVTGLLGSGRTEIAEALFGVRPASSGTVKLDG
                     KPVGLRSIQDAIDAGIGYVPADRLTQGLFMDRAIGDNIIAGSLDRYRTRWGTLDNSKT
                     RKTIRELFDRLRIQAPRVTAPVRSLSGGNAQRVVLAKWLAIRPRVLILNGPTVGVDVG
                     SKEDILEILRGEAADGRSIIIISDDVPELVAACHRVLVVRQGRISHTLESDDINVDLI
                     LERMAA"
     gene            672..1730
                     /locus_tag="VGP27_25615"
     CDS             672..1730
                     /locus_tag="VGP27_25615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854714.1"
                     /translation="MNNPQPQRFSLPNLRGPNNEGILALILLGLIILMSLLNPVFFSV
                     STLFAIVRSSIVPLIFALGVLLVIISGGIDVSFAAIAIFAGYTTVTAFGSGAFGAGLV
                     GAILLALAIGAALGLINGVVIARFRLPTLIVTLGTQGIFKGALLTFVGSRFIADLPPA
                     IAGLSTTNLISVATASGRAYLHVMVVPAVLLCFATAWMLRRTMFGRSIYAIGGDVEAA
                     RRAGFRVVRAQILLYVLVGMTAALGGLIYIIMGRSASPQNLVGTELDIIAAVVLGGAS
                     IFGGRGSVTGTVLGVLLVQVIGNSLILAGVPSAWQRAAVGTLLVIGVGVQAVSARRRR
                     RRQPTADGSALTQEAMPV"
     gene            1727..>2049
                     /locus_tag="VGP27_25620"
     CDS             1727..>2049
                     /locus_tag="VGP27_25620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854715.1"
                     /translation="MTGLGQLNNDVGTRTSEFIERLHVSRGTGRMLALLLVAFVFFAA
                     LRPNVFLSPINLQNLALAAPEVGVLAIAMMLAMLTGGIDLSLVNIANLSAITMSTLYT
                     TIHGS"
BASE COUNT          297 a          700 c          655 g          397 t
ORIGIN      
        1 ggcgtcagct tcgccgtccg ggaaggcgag atcctcggcg tgacaggcct gctcggctcg
       61 gggcgcaccg agatcgctga ggctctgttc ggcgtccgac ccgcctccag cggcaccgtc
      121 aaactcgacg gcaagccggt aggcctgcgg tcgatccagg acgcgatcga tgcgggtatt
      181 ggctacgtcc ccgcggaccg cctcacgcag ggtctgttca tggaccgcgc catcggggac
      241 aacatcatcg ccggatcgct cgaccgatac cgaacgcgct ggggcacttt ggataacagt
      301 aagacccgca aaacgattcg cgagttgttc gaccgcctgc gcatccaggc gccccgcgtc
      361 acggccccgg tccgctcgct ctcgggcggc aacgcccagc gtgtcgtcct ggccaaatgg
      421 ctcgccatca gaccacgtgt actcatcctc aacgggccca ccgtcggggt tgacgtcggc
      481 tcgaaggagg acatcctcga gattctgcgc ggggaagccg ccgacggccg ctccatcatc
      541 atcatctccg acgacgtacc cgagctggtc gcagcgtgcc accgggtgct cgtggtccga
      601 cagggtcgga tcagccacac cctggagagc gacgacatca acgtcgacct catcctggag
      661 aggatggcgg cgtgaacaat ccgcagccgc agcgcttctc gctaccgaac cttcgggggc
      721 ccaacaacga gggcattctg gcgctgatcc tgctgggcct gatcatcctg atgtcgttgc
      781 tcaacccggt cttcttctcg gtgtcgacgt tgttcgccat cgtgcgcagc tcgatcgtgc
      841 cgttgatctt cgcgctcggc gtgctgctgg tcatcatctc cggcgggatc gacgtctcct
      901 tcgccgcgat cgcgattttc gccgggtaca cgaccgtgac ggcgttcggg tcgggcgcct
      961 tcggtgccgg gcttgtcggg gcgatcctgc tcgccttggc gatcggcgca gcgcttggcc
     1021 tcatcaacgg cgtcgttatc gcgcggttcc ggctgcctac gctgatcgtg acgcttggaa
     1081 cgcagggcat cttcaagggc gccctgctca ccttcgtcgg ttcgcgcttc atcgccgacc
     1141 tcccgcccgc catcgccggg ttatcgacca cgaacttgat ttcggtcgcg actgccagcg
     1201 gccgcgccta tctacatgtg atggtcgttc ccgccgtcct gctgtgcttc gcgacggcat
     1261 ggatgttgcg ccgcaccatg ttcggccgtt ccatctacgc gatcggcggc gatgtggagg
     1321 cggcgcgccg cgccggcttc cgggtggtcc gggcccagat cctgctctac gttctcgtgg
     1381 gcatgaccgc agcactcggt ggactgatct acatcatcat gggccgcagc gccagcccgc
     1441 agaacttggt cggcaccgag ctcgacatca tcgcggcggt ggtcttgggg ggtgcgtcga
     1501 tcttcggcgg tcgcggttcg gtgaccggca ccgttttggg tgtgttgctc gtgcaggtca
     1561 tcggcaacag cctgatcctg gccggtgtcc ccagcgcatg gcaacgcgcc gcggtcggga
     1621 ccctgctcgt catcggtgtc ggcgtgcagg ccgtgtcggc caggcggcgc cgcaggcgtc
     1681 agccgaccgc cgacgggtcc gccctgacgc aggaggcgat gcctgtatga cgggtctcgg
     1741 gcagctcaac aacgacgtgg gcactcgcac ttccgaattc atcgaacggc tccatgtcag
     1801 ccgaggcacc ggccgcatgc tggcgcttct tctggtggcc ttcgtgttct tcgcggcgct
     1861 gcggcccaac gtcttcctca gcccgatcaa tttgcagaac ctcgcactcg ccgcgccgga
     1921 ggtcggcgtg ctcgcgatcg cgatgatgct ggccatgctg accggcggga tcgacctgtc
     1981 gctggtcaac atcgccaacc tgtcggcgat cacgatgtcg accctgtaca cgacgatcca
     2041 cggctcgaa
//
LOCUS       DASYLX010001095         7465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2057012,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001095 DASYLX010000000
VERSION     DASYLX010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057012"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGP27_25625"
     CDS             complement(<1..426)
                     /locus_tag="VGP27_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854716.1"
                     /translation="MTSSVLINPATEQELRTVERVDEAGVDEAVARAVVAQRSWAALP
                     PADRAAALRAFASVVDAHVDELAALEVANSGHPIGNAEWEAGHVRDVLQFYSASPERL
                     SGKQIPVAGGLNVTFNEPLGVIGVITPWNFPMPIASWGFA"
     gene            complement(423..1232)
                     /locus_tag="VGP27_25630"
     CDS             complement(423..1232)
                     /locus_tag="VGP27_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV7854717.1"
                     /translation="MSGFDARSEGASRRYDPAPVIGLTTYLQQAQTGIWDVHASFLPA
                     IYMEGVNLAGGTAVLLPPQPVEAAERVLSGLDGVVITGGRDVDPAAYGQQRHPATDEP
                     VQANRERDAWEIALVRGAIRRGIPLLGICRGAQVVNVALGGTLHQHLPDVVGHTRHQQ
                     GNAVFSTSAVHTVPGSRLASLVGDTSDVQCYHHQAIDRLGQGLVVSAQDDDGVIEAVE
                     IPSGTVEAAGQGSDNFVLAVQWHPEERLDDIRLFVAVVHAAAEYATGKVNA"
     gene            complement(1219..2568)
                     /locus_tag="VGP27_25635"
     CDS             complement(1219..2568)
                     /locus_tag="VGP27_25635"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV7854718.1"
                     /translation="MLSQTELESLVAAGDIDTVIVAFCDMQGRLTGKRVAARLFVEDV
                     AAHGAECCNYLLAVDVDMNTVDGYAISSWETGYGDMVMTPDFSTLRLLPWLPGSALVM
                     ADLSWTDGKPVTQAPRSILNKQIARLADKGLVPFIGTELEFMVFDDSYREAWAAGYRG
                     LKPATDYNVDYAMLASTRMEPLLRDIRLGMDGAGMYCEGVKGECNFGQQEIAFRYDHA
                     RITCDNHTIYKNGAKEIADQHDKSLTFMAKFDEREGNSCHIHISLRGDDGSAVFADSG
                     ASDGMSEMFRSFIAGQLATLRELTLFYAPNINSYKRFVEGSFAPTAIAWGMDNRTCAL
                     RVVGHGHGMRMECRAPGGDVNQYLAVSALIAGGLHGIEQELELPDALVGNAYTSGAET
                     LPTTLTEAAELFGESKVAREAFGDDVVEHYLNYAHVELKAFNSAVTDWERVRGFERL"
     gene            complement(2611..3351)
                     /locus_tag="VGP27_25640"
     CDS             complement(2611..3351)
                     /locus_tag="VGP27_25640"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7854719.1"
                     /translation="MPGQTLLADRLRDELLEEITSAQLEPGTKLPTEGELSKRFGVSR
                     ATVREAVRGLVEAGYVIRRRGSGSYVAERRRMPHGLDSTLSYLAMIESAGAHAGMRVL
                     DAVFEQSSKIDGPLQVGRGEKVLAVERVRTADEQPVIYSRDRIPARLLRDDLDLENLD
                     PSLFALLSSSGHAADHATATLRAVASTGFTAKVLGIRRGKPLLYIEEIDYDRDGTPVM
                     LSQEWHVSEAFDVRINRQARTAPISTQR"
     gene            complement(3361..4056)
                     /locus_tag="VGP27_25645"
     CDS             complement(3361..4056)
                     /locus_tag="VGP27_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV7854720.1"
                     /translation="MAHFVFMLTHGDRTVDNAAEIIPTLADSGLRYIGFKDVGADLAR
                     QRELTELAHDAGFEVMLEVVSTSKEAEMASLRSAAEAGFDWVLGGTHGAETAGMLPDG
                     VKYCPFPGRVVDHPSVLLGTVDDIAADAEELTALDHVSGLDLLAYRHPSADPLELIRR
                     VSASSRGPVIVAGSIASVERAVAVSEAGAWGYTIGSAIFDGLLPGAPSIADQVRRVLE
                     ETASAAGAAQGTT"
     gene            complement(4067..5419)
                     /locus_tag="VGP27_25650"
     CDS             complement(4067..5419)
                     /locus_tag="VGP27_25650"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV7854721.1"
                     /translation="MNTTVPEPNAILADLRGRGHDDLPGVDIRVGTSVLDRAIADACA
                     RPGTPAPVIISDGGPYRTPAGPVVDRLAKKLGCPVIQLGTGSVRADEETVGRAVAEVG
                     SVRLLISVGAGTLTDIAKVTAQRTGSRHVAVQTACSINGFIADRSVLVIAGAKRTVVS
                     RWPDMLVADSDILTAAPAGLNLAGVGDLSTVPNAVAEWQLAAALGHGPAYDAAVVEQV
                     LAAMPALPRLAQATRDAEPGGLADLARLLAGSGLSMGIVGSTAPASGSEHAVSHLLEM
                     ALSQRQGPAAPHGMQVTVATRLALRVWQLVDRTISSGQARLQVPDETASRDAVALAFA
                     ELGAKTVDECWTAYSAKRNWLQEHLSDVEALVADWNGFKRTLTLPTPEQFDEVSHASG
                     LPTRARDLGAGYDDRLLFWALRNSCLLRERISVVDLADLLGVWSDDMARSIVADAERE
                     "
     gene            complement(5416..6912)
                     /locus_tag="VGP27_25655"
     CDS             complement(5416..6912)
                     /locus_tag="VGP27_25655"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV7854722.1"
                     /translation="MSLVLGVDVGSQSIKAVIVDEAGDVVSSGSAPLTMVHEHDGWAD
                     QDPYAYCCALRGAVRAATDGVQADRVVAMGLGSQVDGVVACDAVGAPLRAAIIWLDKR
                     ATKQCDQLVSAVGADLLAERTGLVADASHSAPKMMWLRDNEPQVWETAAMLAPVGSYV
                     LHYLSGEHAQDAANASSTMVYDVTKGDFDSELCEAAGLDPAKLPHVRPSTDVVGTLRA
                     DVAADLGLPATCAVVVGTGDEHAASVGAGAIESGVVVDVTGTAEPVTTVGAEPVRDPL
                     GLVETHGHAVPGSWLIENPGFVSGGSTLWLAGVLGISQGEIFTLARKAPPTSDGVLFL
                     PALSGSTAPRWNDAMRGALLGLAMNHTSAHAARSVLEGCAYALRDIVDRLNALGLGHG
                     EVRIVGGGARDDLWASIKADVLGRPVRRVLTEEATAVGAAMVAGVGAGVFADFSAASV
                     AVQLDPDTIDPDPTAQRQYADAYGRYLAAFDAVESSLAPGPESSARPT"
     gene            complement(7004..>7465)
                     /locus_tag="VGP27_25660"
     CDS             complement(7004..>7465)
                     /locus_tag="VGP27_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854723.1"
                     /translation="SENALAGKIVGALLYMAVFGAVISYFMQCLAFIVLRRKLPDIER
                     PYRSPVGVWGAAIAGAIALIALVSLFLNEDYRPGVYGTLVYFVIGIIYFAVRGRHKLV
                     LSPEEEFAMSHGKRGANLQEEGYGTVSVTEISADVDSASPTRADDSPTRSP"
BASE COUNT         1187 a         2600 c         2484 g         1194 t
ORIGIN      
        1 ggcgaaaccc cacgatgcga tcggcatcgg aaaattccac ggggtgatga cgccgatgac
       61 cccgagcggc tcgttgaagg tgacgttgag tccacccgcc accggaatct gcttgccgga
      121 caggcgttcc gggctggcgg agtagaactg cagcacgtcg cggacgtgtc ccgcctccca
      181 ctcggcattg ccgatcggat gtccggagtt ggcgacctcg agcgccgcga gctcgtcgac
      241 gtgcgcgtcg acgacggacg cgaacgcccg aagcgcggcg gcccgatcgg cggggggcaa
      301 cgcggcccac gaacgttgcg cgacgacggc gcgcgccact gcctcgtcga cgccggcctc
      361 gtcaacccgc tcgacggtgc gcaactcctg ctccgtggcg gggttgatca gaacagacga
      421 agtcacgcat tcacctttcc tgtcgcgtat tccgcagcgg cgtggaccac cgccacaaac
      481 aaccggatgt cgtcgaggcg ttcctcggga tgccattgca cggcgagaac gaagttgtcc
      541 gacccctgtc ccgccgcttc gaccgtcccg gacgggattt cgaccgcctc gatcaccccg
      601 tcgtcatcct gcgcgctgac gacaaggccc tgccccagcc ggtcgatggc ctgatggtgg
      661 tagcactgaa cgtccgacgt gtcgccgacc agcgaggcca gcctgctgcc cggcaccgtg
      721 tgcacggccg acgtggagaa caccgcgttg ccctgctgat ggcgggtgtg gcccacgacg
      781 tcgggcaggt gctggtgcag cgtgccgccg agcgccacgt tgaccacctg cgcgccgcgg
      841 cagatgccga gcagcgggat gccgcgccgg atcgcaccgc gtaccaacgc gatctcccag
      901 gcgtctcgct cacgattggc ctgcaccggc tcgtcggtgg ccggatgacg ctgctggccg
      961 taggcggccg gatccacgtc ccgcccaccg gtgatgacca caccgtccag cccgctcagc
     1021 acccgttcgg cagcctcgac gggttgcgga ggcaggagca ccgcggtgcc gcccgcgagg
     1081 ttgaccccct ccatgtagat ggcgggcaga aaactggcgt gcacatccca gatgcccgtc
     1141 tgcgcctgct gcagataggt cgtcaggccg atgacgggcg cggggtcata tctgcgcgat
     1201 gcgccttcgc ttctcgcgtc aaagccgctc aaagccgcgc accctctccc agtcggtgac
     1261 cgcggaattg aatgccttca gctcgacgtg ggcgtagttg aggtagtgct cgacgacatc
     1321 gtcaccgaag gcctcgcgcg cgaccttgga ctctccgaac agctcggcgg cctcggtcaa
     1381 cgtggtcggc agggtctccg cgccgctcgt gtaagcgttg cccaccaacg catccggcaa
     1441 ctcgagctcc tgctcgatgc cgtgcagacc gcccgcgatc aaggccgaaa ccgccaggta
     1501 ctggttgacg tcgccaccgg gcgcccgaca ctccatccgc ataccgtgac cgtggccgac
     1561 gacccgcagc gcgcatgtcc ggttgtccat tccccatgcg atcgccgtcg gtgcgaaact
     1621 gccctcgacg aatcgcttgt aggagttgat gtttggcgca tagaacagcg tcagctcacg
     1681 aagcgtcgca agctgaccgg cgatgaagct ccggaacatc tccgacatgc cgtcgctcgc
     1741 gccggagtcg gcgaacaccg cgctgccgtc gtcgccgcgc agcgagatat ggatgtgaca
     1801 gctgttgcct tcgcgctcat cgaatttggc catgaacgtc aggctcttgt cgtgctggtc
     1861 ggcgatctcc ttggcgccgt tcttgtagat cgtgtggttg tcgcacgtga tgcgggcgtg
     1921 gtcgtagcgg aatgcgatct cctgctggcc gaagttgcat tcgcccttga cgccctcgca
     1981 gtacatgccc gcgccgtcca tgccgagccg gatgtcgcgc aacagcggct ccatccgggt
     2041 ggacgccagc atcgcgtagt cgacgttgta gtcggtggcc ggcttcaggc cgcgataccc
     2101 ggcagcccac gcctcgcgat aggagtcgtc gaagaccatg aactccagtt cggtgccgat
     2161 gaacgggacc agtcccttgt cggccagtcg ggcgatctgc ttgttgagga tgctgcgcgg
     2221 cgcctgcgtc accggcttgc cgtcggtcca cgacaggtcg gccatcacaa gcgcggatcc
     2281 cggcagccac ggcagcaggc gcagcgtgga gaagtccggc gtcatcacca tgtcgccgta
     2341 gccggtctcc cagctcgaga tcgcgtagcc gtcgacggtg ttcatgtcga cgtcgacggc
     2401 gagcaggtag ttgcagcatt ccgcgccgtg ggccgcgacg tcttcgacga acagccgtgc
     2461 ggcaacccgt ttgccggtca gccggccctg catatcgcag aacgctacga tcacggtgtc
     2521 gatgtctccg gccgcgacca acgactccaa ctcggtttgc gacagcattc caggcactcc
     2581 ctaaaggtag gtcatccgac cattagaccg ctagcgctgc gtggatatcg gcgccgttcg
     2641 cgcctgccgg ttgatccgga cgtcgaacgc ctcggacacg tgccattcct gcgacagcat
     2701 caccggcgtg ccgtcgcggt cgtaatcgat ctcctcgatg tagagcaggg gcttgccgcg
     2761 tcggatcccg agcaccttgg cagtgaagcc ggtgctggcg acggcgcgca gggtcgccgt
     2821 cgcgtggtcg gcggcatggc ccgaggagct cagcagcgcg aacaacgagg ggtcgaggtt
     2881 ctcgaggtca aggtcgtctc tcagcagccg ggcggggatg cgatcgcgcg agtagatcac
     2941 cggctgctcg tcggcggtcc ggacgcgttc caccgccagc accttctcgc ccctgcccac
     3001 ctgcagcggg ccgtcgatct tcgaggactg ctcgaacacg gcatcgagca cccgcatccc
     3061 ggcgtgcgcg cccgcgctct cgatcatcgc gagatagctc aatgtcgagt ccagcccgtg
     3121 cggcatgcgc cgacgttcgg cgacatagct tcccgacccg cggcgccgga tgacgtagcc
     3181 ggcttcgacg aggccgcgta ccgcttcgcg caccgtagcc cgcgacaccc cgaaccgctt
     3241 ggacagttcg ccctcggtgg gcagtttggt tccgggctcc agttgcgcgc tggtgatctc
     3301 ctccagcagc tcgtcgcgca accggtcggc gagcaacgtc tgccctggca tttcatgagc
     3361 ctacgtggtg ccctgcgcgg caccggcggc cgacgcggtc tcctccagca cccggcggac
     3421 ctggtctgcg atggacggcg cacccggcag caggccgtcg aagatcgcgc tgccgatcgt
     3481 gtagccccac gcgcccgcct cgctgaccgc aacggcgcgt tccacgctgg cgatggagcc
     3541 cgcgacgatc accgggccgc gcgaactcgc gctgaccctg cggatcaact cgagcgggtc
     3601 ggcgctcggg tggcgatatg ccagcaggtc cagcccgctg acgtgatcga gtgccgtcaa
     3661 ctcttcggcg tccgcggcga tgtcgtcgac cgtgccgagc agcacgctcg ggtggtcgac
     3721 cacccggccg ggaaacgggc agtacttgac gccgtcggga agcatgcccg ccgtctccgc
     3781 gccgtgggtg ccgccgagca cccaatcgaa gccagcctca gcggccgacc ggagcgacgc
     3841 catctcggcc tccttgctgg tggacaccac ctcgagcatc acctcgaacc cggcgtcgtg
     3901 ggcgagctcg gtcagttcac gttgccgcgc gaggtccgct ccgacgtcct tgaagccgat
     3961 gtagcgcagc ccgctgtcgg cgagggtggg gatgatctcg gcggcgttgt cgacggttcg
     4021 gtcgccgtgg gtcaacatga aaacaaaatg ggccattggt tctccttcat tctctttcgg
     4081 cgtcggcgac gatcgaccgc gccatgtcgt ctgaccagac tccgagcagg tcggcgagat
     4141 cgacgaccga aatccgttcg cgtagcagac aagagttgcg cagcgcccag aacagcagcc
     4201 ggtcgtcgta gccggcgccc agatctcggg cccgggtggg aaggccgctg gcgtggctca
     4261 cttcgtcgaa ctgctccggg gtgggcaagg tcagcgtccg cttgaatccg ttccagtcgg
     4321 cgaccagcgc ctcgacatcc gacagatgct cctgcagcca gttccgcttc gcggagtagg
     4381 ccgtccagca ctcgtcgacg gtcttcgcac ccagttcggc gaaggcgagg gccaccgcgt
     4441 cgcggctggc cgtctcatcg ggcacctgca gccgggcctg tcccgacgag atcgtccggt
     4501 cgaccagttg ccagacccgc agcgcgagcc gggtcgccac ggtcacctgc atgccgtgtg
     4561 gcgcggcggg cccctgccgt tgggacagcg ccatctccag caggtgcgac accgcgtgtt
     4621 cgctgcccga tgccggagcg gtcgagccga cgatccccat cgacagcccg ctgccggcga
     4681 gcagccgggc caggtcggca agtccgcctg gctcggcgtc gcgggtcgcc tgcgcgagcc
     4741 tcggcagtgc gggcatcgcg gcaagtacct gttccacgac cgcggcgtca tacgccggtc
     4801 cgtgaccgag ggccgccgcg agttgccact cggcgaccgc gttgggaacc gtggacaggt
     4861 cgcccacccc ggcgaggttc agccccgcag gggcagcggt gaggatgtcg gagtcggcga
     4921 cgagcatgtc gggccatcgc gagacgaccg tgcgtttcgc accagcgatg accaaaacgc
     4981 tgcggtcggc gatgaaaccg ttgatgctgc acgctgtttg gaccgcgacg tggcggctac
     5041 cggtgcgctg cgcggtgacc ttcgcgatgt cggtcagcgt gccggcgccg accgagatca
     5101 gcagtcgcac ggagcccacc tcggccaccg cgcgcccgac cgtttcctcg tcggcgcgca
     5161 cggagcccgt gcccagctgg atcaccgggc agccgagctt cttggcgagc cggtcgacga
     5221 ccggtcctgc cggcgtgcgg taggggccgc cgtcactgat gatcaccggc gcaggcgtac
     5281 ccggccttgc gcaggcatcg gcgatggcgc ggtccagaac cgacgtgccg acgcggatat
     5341 cgacgccggg cagatcgtcg tggcccctgc cgcggaggtc ggcgaggatg gcgttgggct
     5401 caggcacggt ggtgttcacg tcggccgagc cgatgattcg gggcccggcg ccagcgacga
     5461 ttcgacggca tcgaacgccg cgaggtagcg cccgtaggcg tcggcatact gcctctgcgc
     5521 ggtggggtcc gggtcgatgg tgtcgggatc cagttgcacg gctaccgatg ccgccgagaa
     5581 gtcggcgaac actccggctc cgactcccgc caccatcgcc gcgccgacgg cggtggcctc
     5641 ttcggtcagc acacggcgca ccggcctgcc gagcacatcg gccttgatcg acgcccacag
     5701 gtcgtcgcgc gcaccgccgc cgacgatccg gacttccccg tggccgaggc cgagcgcgtt
     5761 gagccggtcc acgatgtcgc gaagtgcgta cgcgcaccct tcgagtaccg aacgtgccgc
     5821 gtgtgccgag gtgtggttca tcgccagccc gagcaatgcg ccgcgcatcg cgtcgttcca
     5881 gcgcggggcg gtcgagccgg acagcgcggg caggaacagc acaccgtcgc tggtcggtgg
     5941 cgccttccgc gcgagcgtga agatctcgcc ctgagatatg ccaagcactc cggccagcca
     6001 cagcgtgctg ccgccgctga cgaatccggg gttttcgatc agccacgaac cgggcaccgc
     6061 gtgcccgtgc gtctccacca gaccgagtgg gtcgcggacg ggttcggcgc cgaccgtcgt
     6121 caccggttcc gcggtgcctg tgacgtccac gacgaccccc gactcgatag cgcccgcccc
     6181 aaccgatgcg gcgtgctcgt ctccggtgcc gacgaccacc gcacaggtgg caggcagccc
     6241 gaggtcggcg gcgacgtcgg cgcgcagggt gccgaccacg tccgtcgacg gccgcacgtg
     6301 gggcagcttg gccgggtcga ggcctgccgc ctcacatagc tcgctgtcga aatcgccctt
     6361 ggtcacgtcg tacaccatcg tcgacgacgc attggcggca tcttgggcat gctcacccga
     6421 aaggtaatgc aggacataag atccgacagg ggcgagcatg gcggcggtct cccacacctg
     6481 cggttcgttg tcccgcagcc acatcatctt cggggcgctg tgggacgcgt cggccaccaa
     6541 gccggtgcgc tcggccagca gatcggcccc caccgccgag accagctggt cgcattgttt
     6601 ggtggcacgc ttgtcgagcc agatgatcgc cgcgcgcagc ggtgccccga ccgcgtcgca
     6661 tgcgacgact ccgtcgacct ggctgcccag ccccatcgcc acgacccggt cggcctgcac
     6721 gccgtcggtt gccgcgcgca cggcgccgcg aagtgcgcag cagtaggcgt acgggtcctg
     6781 gtcggcccag ccgtcatgct cgtgcaccat ggtcaacggc gccgacccgg aggagacgac
     6841 gtcgcctgcc tcgtcgacga taacggcctt gatgctctgc gagccgacat cgactcccag
     6901 gaccagagac acgactcccc ctcagattag gcggtagagc ttgagcactg aaagttacgg
     6961 tatgcgggga ggattgggcg ggaatcctcc ccgccggtca tgactatggt gaccgagtcg
     7021 gcgaatcgtc ggcccgcgtc ggagacgccg aatcgacatc cgccgagatc tcggtgacgg
     7081 agaccgtacc gtaaccctcc tcctgcaaat tcgctccgcg cttgccgtgg gacatcgcga
     7141 actcttcctc aggtgagagc accagcttgt ggcggccacg gaccgcgaag tagatgatgc
     7201 cgatcacgaa gtagaccagc gtgccgtaga caccgggccg gtagtcctcg ttgaggaaca
     7261 gcgacaccag ggcgatcaga gcgatcgcac ccgcaatcgc cgcgccccac acgccgacgg
     7321 ggctgcggta cggacgctcg atgtcgggca gcttgcggcg cagcacgatg aaggccaggc
     7381 actgcatgaa gtacgagatc accgcaccga acacggccat gtacagcagc gcgccgacga
     7441 tcttgccggc aagggcgttc tcgct
//
LOCUS       DASYLX010001096         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_741133,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001096 DASYLX010000000
VERSION     DASYLX010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741133"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VGP27_25665"
     CDS             <1..1498
                     /locus_tag="VGP27_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV7854724.1"
                     /translation="LACLTLGDAGAAVILERGTGDRVGFHDIDMATLGAYSKLCVAKT
                     TDGPHGGAVMFTDSIAGTAVAVRAAVPYVAAVMRRHGWRPQSADHLVIHQTSESSLND
                     AMLAINRVFGKSAAHLGNTIFNLTERGNTASTSHWVALKDHILSNRIQSGDRTVFGIS
                     GSGQTVGAALYTFDDLPDRLRRGSNGQRAVGLSSGRRPTELPATPRVRVVGVGTVPAH
                     VSTTQGSVANGVHAAKACLDASGVAPSEVGLLIHAGVYRDEFISEPAIAAFIAGDIGA
                     NEDIQGADGAKTLAFDVLNGGVGFLNACQVATQMIGGGKVDHAMVVVAETENNTAESG
                     DPLLGVEQTGSAVLLSGGDDSVGFGRFVFHHDPEHADALTTYTRQAQGHTWLHIDREP
                     KLTTHYLDCIPAAVDELLKVEGLDRSDISVVIPPFLSAGDRTELATRIGIDSTRFVEF
                     QGEADPFTSSVPLGLQQAWRHELVKPGDICLIITVGSGLQVGCTTYRF"
     gene            1507..2835
                     /locus_tag="VGP27_25670"
     CDS             1507..2835
                     /locus_tag="VGP27_25670"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854725.1"
                     /translation="MTQTAALTETRTAMKPVGKFTADLLKVGVDSLAGVVGDTVIERV
                     RRRNGAADPVTRFNPFKRETILDPYPEYEQLLAGPPVRYNPRLGIWIVPRFTDVRAGL
                     RDHERLSSSQSQSRFRVALPMMIAKDPPDHTRLRQLVSRAFTPRALAAWQTLIEQVCD
                     ELLDRMLSMPNPDGVQDLAKPLPTRIIATMFGIPEADQMKFLSWSEALIDGSFVEVGR
                     RTPNVVQKMARATFAMRKYVRPLVDERRRNPGTDLISMMAQAEDGDVLDDDEIFWGVV
                     MLIVAGNETTTNLLGWLLHTFATRHDIFDELRGKPELIPTAIEEQLRLESPVQGFYRT
                     ATVDYPVEHLVIPKGSRAMMLFGAANRDPRHFPDPNTFRLDRGAVDHLALGGGVHFCL
                     GAGLSRMEAGQIIAHLSKRVAAVELVGQPQRLHNATMRGLTHLPLRMVGA"
     gene            2875..3633
                     /locus_tag="VGP27_25675"
     CDS             2875..3633
                     /locus_tag="VGP27_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7854726.1"
                     /translation="MTKNNGEVLGAPVIEARDLSKSFGRGDTETPILENIDLQIFSGD
                     FVVLIGPSGSGKSTLLSILGLLEPPSTGDVLVAGQSVKKLSRRQLARLRGRKLGYVFQ
                     SFNLLAGLSVVENVMLSGLLVGESGRTQHDRAIELLTEFGLADKAKRVPSELSGGEQQ
                     RVAIARALFMKPDVILADEPTGNLDTKNGRKVIEALENLNREGQTIVLVTHDLSIAEN
                     APRLIAFRDGQIESDNRGTGRHRLEADDLQRVAP"
     gene            3636..>4447
                     /locus_tag="VGP27_25680"
     CDS             3636..>4447
                     /locus_tag="VGP27_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854727.1"
                     /translation="MAGRRIAATVSVLRIINMRALRRHAFRALLAAISLGGGVAVVVA
                     VMIEATSVTKAVEDVGYRIAGPAPMRILGAANRAGVTPEAMRTAATTPGVTAVVPVVR
                     AITMVRTGDRETYVLALGIDCTARWIIDPAVCPPGQQEPPLPATSTILSKSLEPSSRL
                     VTDVGQMSLTGLQQVSQLDTINHGMVVVLPLRAAQAQFARGDRVDMAYLTLVDDATAP
                     QIGTQLQQSLGPGFDVLTRSEPDRGFNVNIVLLPLLAIFALIAVGVGVILIA"
BASE COUNT          809 a         1489 c         1380 g          769 t
ORIGIN      
        1 tcttgcgtgc ctgaccctcg gcgacgccgg ggcggcagtg attctcgagc gtggcacagg
       61 tgaccgtgtc ggcttccatg acatcgacat ggccaccctc ggcgcctaca gcaagctctg
      121 cgtggccaag acgaccgatg ggccgcacgg cggagccgtc atgttcaccg actccatcgc
      181 gggaacagcg gtcgccgtca gggcggcggt gccctatgtc gcggccgtga tgcggcgaca
      241 cggctggcgt ccgcagagtg ccgaccacct cgtcatccac cagacgtcgg aatcgtcgct
      301 caacgatgcg atgctcgcga tcaaccgggt gttcggaaag tccgccgccc atctcggcaa
      361 caccatcttc aacttgaccg aacgcggcaa caccgcgagc acttcgcact gggtggccct
      421 caaggatcac atcctgagca accggatcca gtccggtgac cgcaccgtct tcggcatcag
      481 cgggtcgggc cagacggtcg gcgccgcgct ctacacattc gacgacctgc ccgaccggtt
      541 gcgccgcgga tccaacggcc aacgcgcagt cggtctttcg agcggccggc ggcccacaga
      601 gcttcccgcg actccgcgtg tgcgcgtcgt gggagtcggt accgttcccg ctcatgtgtc
      661 gacgactcag ggttcggtgg ccaacggtgt gcatgccgcc aaggcgtgcc tcgacgccag
      721 cggtgtcgcc ccgtcggaag tcggcctgct catccacgcg ggcgtttacc gtgacgagtt
      781 catcagcgaa cccgccatcg ccgcgttcat cgcgggcgac atcggcgcca atgaagacat
      841 tcaaggggcc gacggcgcaa agactctcgc cttcgatgtg ctcaacggcg gagtcggctt
      901 cctgaacgcc tgccaggtcg ccacccagat gatcggcggc ggcaaggtcg accacgcgat
      961 ggtggtggtc gcggagacgg agaacaacac cgccgaaagc ggcgatccac tgctcggcgt
     1021 cgagcagacg ggatccgccg tgcttttgag tggcggcgac gactcggtcg gcttcggtcg
     1081 gttcgtgttc catcacgacc ccgagcacgc cgacgcgctg accacataca cgcgccaggc
     1141 acaggggcac acctggttgc acatcgaccg cgaaccgaag ctgacgaccc actacctgga
     1201 ctgcatcccc gccgcagtcg acgaactgct gaaggtcgag ggacttgacc gctcggacat
     1261 ctcggtggtg attccaccgt tcctgtctgc gggagaccgc accgagttgg ccacccgtat
     1321 cggcatcgac agcacgcgct tcgtcgaatt tcaaggcgag gcagatcctt tcacctcgtc
     1381 ggtacccctc ggccttcaac aggcctggcg gcacgagctg gtcaagccgg gcgatatctg
     1441 cttgatcatc actgtcggct cgggtcttca ggtcggctgc accacctacc gattctgagg
     1501 ttggacatga ctcaaaccgc tgctctgacg gagacccgga cggcgatgaa gccggtcgga
     1561 aagttcaccg ctgatctgct caaggtcggt gtcgacagcc tcgcaggcgt ggtaggagac
     1621 acggtcatcg aacgtgtccg gcgtcgcaac ggcgccgccg acccggtgac acgcttcaat
     1681 ccgttcaagc gtgaaaccat cctcgacccc taccccgaat acgagcagct tctcgccggg
     1741 ccgccggtgc gctacaaccc gcggctcggc atttggatcg tgccacgatt caccgacgtg
     1801 cgggcagggt tgcgtgacca cgagcgcctg tcctcgtcac agagccagag ccggttccgc
     1861 gtggcattgc cgatgatgat cgccaaggat ccacccgacc atacgcggct gcgccagttg
     1921 gtatctcgtg ctttcacgcc ccgtgcgctc gcggcatggc agaccttgat cgagcaggtc
     1981 tgcgacgaac ttctcgaccg gatgctcagc atgccgaacc ccgacggcgt gcaagacctg
     2041 gccaagccgc tgcccacccg gatcatcgcc accatgttcg gcattcccga ggccgatcag
     2101 atgaagttcc tctcctggtc ggaggcattg atcgacggct ccttcgtcga ggtcggcagg
     2161 cggactccca acgtcgttca aaagatggcg cgggctacgt tcgccatgcg aaagtacgtg
     2221 cggccgctcg tcgacgagcg gcgccgcaat cccggcacgg acctgatctc gatgatggcc
     2281 caggcggaag acggagacgt actcgacgac gacgaaatct tctggggcgt cgtcatgctc
     2341 atcgtcgcgg gcaacgagac cacgaccaac ctgcttggct ggctgctgca caccttcgca
     2401 acgcgacacg acatattcga tgagctacgc ggtaagcccg agttgattcc gacggccatc
     2461 gaggagcaat tgcggctcga gtcgccggtg cagggtttct accgcacggc gaccgtcgac
     2521 taccccgtcg agcatctcgt catcccgaag gggtcgcggg cgatgatgct gttcggcgcc
     2581 gccaaccgcg acccgcgaca cttccccgac ccgaacacct tccgtctcga ccgcggagcc
     2641 gtcgaccatc tggcgctcgg cggtggtgtg cacttctgcc ttggcgccgg tctttcccgg
     2701 atggaagcgg gccagatcat cgcgcatctc tcgaagcggg tcgccgcagt ggaattggtt
     2761 ggacagccac agcggctcca caacgcgacg atgcgcggat tgacgcatct tcctcttcga
     2821 atggtgggcg cctgatatgc cgttcgatgc atcaacacaa acaaggattg ggacgtgaca
     2881 aaaaacaacg gcgaggtcct cggcgcaccg gtcatcgaag cgcgagacct ttcgaagtca
     2941 ttcggtcgcg gcgacaccga gacaccgatt ctcgaaaaca tcgacctgca gatcttctcg
     3001 ggtgacttcg tggtgctgat cgggccgtcc gggtcgggga agtcgacatt gctgagcatc
     3061 ctcgggctgc tcgaaccgcc atcgactggc gacgtgctgg tggccggaca gagcgtgaag
     3121 aaactttccc gtcgtcagct ggctcgcctg cgaggccgca agctcggata cgtcttccag
     3181 tccttcaacc tcctcgccgg gctatcggtg gtcgagaacg tcatgctcag tggcctgctc
     3241 gtcggcgaat ccggccgcac ccagcatgac cgggcgatcg agttgctgac cgagttcggc
     3301 ctcgcggaca aggccaagcg tgtgccgtcg gagttgtccg gcggtgagca gcagcgggtg
     3361 gcgatcgcgc gagcgctgtt catgaagccc gacgtgatac tcgccgacga gcccacggga
     3421 aacctcgaca cgaagaacgg tcgcaaagtc atcgaagccc tcgagaatct gaaccgcgaa
     3481 ggtcagacga tcgtcctggt cacccatgat ctgtcgatcg ccgagaatgc gccccggctc
     3541 atcgccttcc gcgacggcca gatcgaaagc gacaaccgcg ggaccggcag gcaccgtctt
     3601 gaagccgatg acctccagcg agtggcgccg taaccatggc gggacgccga atagccgcga
     3661 ccgtcagcgt tctgcggatc atcaacatgc gggctcttcg acgacatgcc ttccgggcac
     3721 tgctggccgc catttcgctg ggtggcggcg tggccgtcgt cgtcgccgtg atgatcgagg
     3781 caaccagtgt caccaaggcc gtcgaagacg tcggttaccg gatcgccggc ccggcgccga
     3841 tgcgcatact cggagccgcg aaccgagcgg gcgtgacacc ggaggcgatg cgaaccgccg
     3901 ccaccacacc gggcgtaacg gcggtggtcc ccgtcgttcg ggccatcacc atggtccgca
     3961 cgggtgaccg cgaaacgtac gtgctggctt tgggcatcga ctgcacggca cgatggatca
     4021 tcgaccccgc cgtatgtccg cccggccagc aggagccccc gctgccggcc acatcaacca
     4081 ttctgagcaa gtcactggag ccgtcgtcga gattggtcac cgacgtcggg cagatgtctt
     4141 tgactgggct gcagcaagtc tcgcaattgg acacgatcaa tcacggcatg gtcgtggtgt
     4201 tgccgctgcg cgcggcccag gcgcagttcg ctcgaggcga ccgggtcgac atggcttacc
     4261 tcacgcttgt cgacgacgcc acggcacctc agataggaac gcagctccag caatcactcg
     4321 gccccggctt cgacgtgctg actcgcagcg aacccgaccg gggtttcaac gtcaacatcg
     4381 tgcttctccc gctgctcgcg atcttcgccc tcatcgcggt aggtgtcggc gtcatcctga
     4441 tcgccca
//
LOCUS       DASYLX010001097         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1287006,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001097 DASYLX010000000
VERSION     DASYLX010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287006"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGP27_25685"
     CDS             complement(<1..821)
                     /locus_tag="VGP27_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854728.1"
                     /translation="MTSTTPIDSDTVEDQPRLSALRLLAGNPVTVASAIVLALVAVIA
                     VTAQWIVPFGINDVDVPNALRPPSGDHWFGTDELGRDVLSRVLVAIQASMRVAVVSVA
                     FAVVVGVTIGLVAGYRGGWLDTVVMRVVDVMFAFPVLLLALAVVAVLGPGVTTTILAI
                     GIVYTPIFARVARASTLSVRVEPFVQVSRTMGTGHLYMLGRHILPNIAGPLIVQTSLS
                     LAFAILSEAALSFLGLGIQPPQPSLGRMIFDSQGFVTLAWWMAVFPGAAIVVTVLA"
     gene            complement(818..1774)
                     /locus_tag="VGP27_25690"
     CDS             complement(818..1774)
                     /locus_tag="VGP27_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854729.1"
                     /translation="MTNPVVRFVARRLLYSIVVMVGVLVVVFMLVHLVPGDPVRIALG
                     TRYTPQAYDALRSASGLDRPVIEQFFGYIGNALTGNLGVSFRNGDPVTVTLLERLPAT
                     VSLAVVGIVVALLIALPAGVWSALHEGRISDAIVRVTSQFGVSIPDFWMGILLIALFA
                     STLHWLPTSGYRPLFDDPGGWLRHIILPGLTVGLVAAAIMTRYIRSAVLEVAAMGYVR
                     TARSKGLPPRVVTVRHTVRNAAIPVLTITGIQLATILGGVIVVEVVFAWPGLGRLVYN
                     AVAARDYPVIQGAVLLIAALFLLINLVVDLLYALVDPRIRLS"
     gene            complement(1771..3261)
                     /locus_tag="VGP27_25695"
     CDS             complement(1771..3261)
                     /locus_tag="VGP27_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7854730.1"
                     /translation="MIFALVACSTGERVDLGGDSSGRLVAAIAGEPDQLDPNKTSAYF
                     SFEVLENVYDTLVEPDAHLEMRPALAESWDVSPDQLTWTFHLRAGVTFHDGSPLTADD
                     IVYTYRRIIDEELANVDKLSAVSDIRATNPETVVLRLKQPTPNLLTNLGGFKGMAIVQ
                     RKNVESGQIATQPIGTGPFAFESQKSGDSISLKANPAYWGGAPKVSGVIFRFIPEKST
                     ALSALQAGEVDWTDSIPTQRVNQLKDDDSVNLAVTPSNDYWYLALNEARKPWNDVRVR
                     QAIAYGIDRDAIIAATSYGTAAKNELAIPKGNYASTDFGKYHYDIEQAKRLLREANAS
                     PHNLDMLVTNEYPETVTAAQIIADNLAPLGITVNIHTVDMATWLDEQNSGHFDMLMMG
                     WLGNIDPDDFYYAQHHTDGTSNAQKYSNPEVDRLLDAGRVETNRDARAEIYRKAATII
                     GDECSYIYLYNPSVIQAWNKGMSGYEARRDKAIRFRTASISESGSQ"
BASE COUNT          584 a         1002 c         1122 g          560 t
ORIGIN      
        1 gccagcacag tgacgacgat ggcggcgcct ggaaacaccg ccatccacca cgcgagtgtc
       61 acgaacccct gagagtcgaa gatcatccgg cccaacgacg gctgcggagg ctggatgcca
      121 agaccgagga atgacagcgc cgcctcggac aggattgcga acgcgagtga cagcgaggtc
      181 tgcacgatca gcggtccggc gatgttcggc aggatgtgcc gcccgagcat gtagaggtgg
      241 ccggtgccca tcgtgcgtga cacctgaacg aagggctcca cccgcacgct gagcgtgctg
      301 gcacgcgcca cgcgcgcgaa gatcggggtg tagacgatgc cgatcgccag gatcgtcgtc
      361 gtcacacccg gacccaggac cgccaccacc gccaaagcga gtagcagcac cgggaaggcg
      421 aacatcacgt cgaccacccg catcaccacg gtgtccagcc agccgccgcg gtagcccgcg
      481 accaggccga tcgtcacacc gaccacgacg gcgaacgcca cactcaccac ggcaacccgc
      541 atcgacgcct ggatggccac caatacccgg gacaggacgt cgcggccgag ttcgtcggtg
      601 ccgaaccagt ggtcgccgct gggcgggcgc agcgcgttcg gcacgtcgac gtcattgatg
      661 ccgaacggca cgatccactg cgcagtcacc gcgatgaccg cgacgagcgc gagcacgatt
      721 gcgctcgcca cggtgaccgg gttgcccgcc agcaatcgca gggccgagag ccgcggttgg
      781 tcttcgacgg tatcgctgtc gatgggggtg gtcgacgtca tgacaaccgg atcctcgggt
      841 ccaccaacgc atacaggagg tccacgacga ggttgatgag cagaaacagc gctgcgatga
      901 ggagcaccgc accctggatg accggatagt cgcgcgccgc gacggcgttg tagaccaacc
      961 ggccgaggcc gggccaggcg aacacgacct cgacgacgat gacgccacca agaatcgtcg
     1021 ccaactggat gccggtgatg gtgagcaccg ggatggccgc gtttcggacg gtgtgccgca
     1081 cggtgaccac ccgcggcggc aatcccttcg accgcgcggt gcgcacgtaa cccattgcgg
     1141 cgacctcgag tacggccgac ctgatgtatc gggtcatgat cgccgcggcg acgagtccca
     1201 cggtcagtcc gggcaggatg atgtgccgca accagccgcc cggatcgtcg aacagcggcc
     1261 ggtatccgga cgtcggaagc cagtgcaggg tcgacgcgaa gagggcgatc agcaggatac
     1321 ccatccagaa gtcggggatc gacactccga attggcttgt gacgcggacg atcgcgtcac
     1381 tgatgcggcc ctcgtgcaga gcggaccaga ctccggcggg cagcgcgatg agcagggcga
     1441 cgacgatccc cacgacggcg agcgacacgg tggccggcaa tcgctcgagc agggtgaccg
     1501 tgacggggtc cccgttgcgg aagctgacac cgaggttgcc ggtcagcgcg ttgccgatgt
     1561 agccgaagaa ctgctcgatg acgggacggt ccaatccgct tgcgctgcgc agtgcatcat
     1621 aggcctgggg tgtgtaccgg gtgccgagcg cgatgcggac cggatcgccg ggaaccaggt
     1681 gcacgagcat gaagacgacg acgagcaccc cgaccatcac gacgatcgaa tacagcagtc
     1741 gtcgtgccac gaaccgcacg accgggttcg tcattgcgac ccgctttcgc tgatgctcgc
     1801 ggtccggaag cgaatggcct tgtcgcggcg cgcctcatat cccgacatgc ccttgttcca
     1861 cgcctggatc actgacgggt tgtagagata gatgtagctg cactcgtcgc cgatgatcgt
     1921 ggcggctttc cggtagatct cggcgcgggc gtctcggttc gtctcgacgc ggccggcgtc
     1981 caacagccgg tcgacttcgg ggttcgaata cttctgcgcg ttgctggtgc cgtcggtgtg
     2041 gtgctgggcg tagtagaagt cgtccgggtc gatgttgccg agccagccca tcatcagcat
     2101 gtcgaagtgg ccgctgttct gttcgtcgag ccaggtcgcc atgtcgacgg tgtggatgtt
     2161 caccgtgatc ccgagcggcg caaggttgtc ggcgatgatc tgggcggcgg tgaccgtctc
     2221 cggatactcg ttggtgacca gcatgtcgag gttgtgcggc gacgcgttcg cttcgcgcag
     2281 taggcgtttg gcctgctcga tgtcgtagtg gtacttgccg aaatcggtag aggcgtaatt
     2341 ccctttcggg atggcgagtt cgttcttcgc ggcggtgccg taactggtgg cggcgatgat
     2401 ggcgtcgcgg tcgatcccgt aggcgatcgc ctgccgaacc ctgacgtcgt tccacggctt
     2461 gcgtgcctcg ttcagcgcga ggtaccagta gtcgttgctc ggcgtgacgg cgaggttgac
     2521 ggagtcgtcg tctttcagct ggttcacccg ctgcgtggga atcgaatcgg tccagtcgac
     2581 ttcaccggcc tgcagggccg acagcgcagt cgatttctcg gggatgaatc ggaagatgac
     2641 gccggacacc ttcggggcac caccccagta ggccgggttg gccttcagcg atatcgagtc
     2701 accgctcttc tgagattcga atgcgaacgg accggtgccg atcggctggg tggcgatttg
     2761 gccgctttcc acgttcttgc gttgcacgat cgccatgccc ttgaagccgc cgaggttcgt
     2821 gaggaggttc ggtgtgggct gcttgaggcg gagcacgacg gtttccgggt tggtcgcccg
     2881 aatatcactg acggcgctga gcttgtcgac gttcgccagc tcctcgtcga tgatccggcg
     2941 ataggtgtag acgatgtcgt cggcggtcag tgggctgccg tcatgaaacg tgacgcccgc
     3001 gcgcagatga aaggtccagg tgagctggtc ggggctgacg tcccacgatt ccgcgagcgc
     3061 aggccgcatc tcgaggtggg cgtcgggttc gacgagggtg tcgtagacgt tttcgaggac
     3121 ttcgaacgag aagtaggcgc tggtcttgtt ggggtcgagt tggtcgggct ccccggcgat
     3181 cgctgccacc aggcgtcccg acgagtcacc gccgagatcg accctttcgc ccgtcgaaca
     3241 ggcaacgagg gcgaagatca gtgcggcg
//
LOCUS       DASYLX010001098         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_410349,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001098 DASYLX010000000
VERSION     DASYLX010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410349"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VGP27_25700"
     CDS             <1..134
                     /locus_tag="VGP27_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-(1-2)-phosphatidylinositol
                     mannosyltransferase"
                     /protein_id="HEV7854731.1"
                     /translation="TAVSEILADPQWAAQMGAAGRRWAVDNWQWRSQAARLQCLLNP"
     gene            complement(136..>1807)
                     /locus_tag="VGP27_25705"
     CDS             complement(136..>1807)
                     /locus_tag="VGP27_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7854732.1"
                     /translation="QIRSVALGLIAEGVAPGDRVAVFSATRYEWPIIDFAILSVGALT
                     VPIYETSSVEQVRHVLSDSGAVLVFAETDAHAEKIDHLKDELPDLRKVLRIDSSGASA
                     LDELTAAGKSVDTGELDARLASIKASDPATLIYTSGTTGRPKGCQLTHSNLVYEIRGA
                     KASFPTLLDKGERLLVFLPLAHVLARAITIAAFANKVTLGFTSDIKNLVPMFGLFKPT
                     LVVSVPRVFEKVYNTAEQNARNDGKGRIFEAAVNTAIEWSQAQDSGGPADARSRKGPS
                     LLLRVKHAVFDRLVYGKLRAALGGDCHASISGGAPLGARLGHFYRGVGLTIYEGYGLT
                     ETSAAITVNQINAIKIGSVGKLLPGNSMRLNDDDELLVRGGVVFAGYWHNEEETKAVF
                     TDGWFHTGDLGAIDDEGFLTIVGRKKEIIVTAGGKNVAPAILEDRLRAHPLISQAMCV
                     GDAKPFIAALITIDPEAWPGWKERNGKNADATVADLADDPDLTAEIDLAVKDANQAVS
                     KAEAIRKFRILPVDFTEDTGELTPTLKVKRKVVAEKFASDIEGLYDRA"
BASE COUNT          287 a          608 c          587 g          325 t
ORIGIN      
        1 ccactgccgt aagcgaaata ctcgccgatc cccagtgggc ggcccagatg ggcgccgcag
       61 gccggcgctg ggcagtggac aactggcagt ggcgcagcca ggccgccaga ctgcagtgcc
      121 tgttgaatcc gtagatcacg ctctgtcgta gaggccctcg atgtcggagg cgaacttctc
      181 cgccaccacc ttgcgcttga ccttcagcgt cggcgtcaac tctccggtgt cctcggtgaa
      241 gtcgacgggc aggatgcgga acttgcggat cgcctcggcc ttcgacaccg cctggttggc
      301 gtccttgacg gcaaggtcga tctcggccgt gaggtcgggg tcgtcggcga ggtccgcgac
      361 cgtcgcgtcg gcgttcttgc cgttgcgctc cttccagccg ggccacgcct cggggtcgat
      421 cgtgatgagc gcggcgatga acggtttggc gtcgccgaca cacatcgcct ggctgatcag
      481 cgggtgcgcg cgcagccggt cttcgagaat ggcgggggcg acgttcttgc cgcccgcggt
      541 gacgatgatc tccttcttgc ggccgacgat ggtcaggaag ccctcgtcgt cgatcgcgcc
      601 gaggtcaccg gtgtggaacc acccgtcggt gaagaccgct ttcgtctcct cctcgttgtg
      661 ccagtagcct gcgaacacca cgccgccgcg caccaacagc tcgtcgtcgt cattgaggcg
      721 catgctgttg cccggcaaca gtttcccaac cgagccgatc ttgatcgcgt tgatctggtt
      781 cacggtgatc gccgcgctgg tctcggtcag cccatagccc tcgtagatgg tcagcccgac
      841 gccgcggtag aagtggccga gccgcgcacc cagcggtgca ccgcccgaga tcgaggcatg
      901 gcagtcgccg ccgagcgcgg cacgcagctt gccgtagacc aaccggtcga acaccgcgtg
      961 cttgacgcgc agcagcagac ttggcccttt tctgctcctc gcgtccgcgg ggccgccgct
     1021 gtcctgcgcc tggctccact cgatcgcggt gttgacggcg gcctcgaaga tccggccctt
     1081 gccgtcgttg cgggcattct gctcggcggt gttgtagacc ttctcgaaca cccggggcac
     1141 cgacaccacc agcgtcggct tgaacaaccc gaacatcggc accaggttct tgatatcgct
     1201 ggtgaacccg agcgtcacct tgttggcgaa cgcggcgatg gtgatcgcac gggccagcac
     1261 gtgcgccagg gggaggaaga ccagcaggcg ctcgcccttg tcgagcaacg tcgggaagga
     1321 cgccttggcc ccacgaatct cgtagacgag gttggagtgg gtcagctggc agcccttggg
     1381 cctgccggtc gtgcccgagg tgtagatcag cgtcgcgggg tcggacgcct tgatgcttgc
     1441 cagccgtgcg tcgagttcgc ccgtgtcgac cgacttgccc gccgcggtca actcgtccag
     1501 cgccgacgcg ccggaactgt cgatacgcaa cacctttcgc agatccggca gttcgtcctt
     1561 caggtggtca atcttttcgg cgtgcgcgtc cgtttcggcg aacaccagaa ccgcaccgga
     1621 atcactgagc acgtgacgca cctgctcgac cgagctggtc tcatagatcg gcacggtcaa
     1681 ggcgccgacc gacaggatcg cgaagtcgat gatcggccac tcgtagcggg tggccgagaa
     1741 cacggccacc cggtcaccgg gcgcaacacc ctcggcgatc aacccgagtg ccaccgagcg
     1801 aatctgc
//
LOCUS       DASYLX010001099        14974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1837174,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001099 DASYLX010000000
VERSION     DASYLX010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14974
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837174"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGP27_25710"
     CDS             complement(<1..375)
                     /locus_tag="VGP27_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7854733.1"
                     /translation="MDSISLSRKQPPAGHLRGSTEYRRLLAALFCAGIATFAQLYSPQ
                     GVLPQISADLGVGAASAALMVSACTIGLAIGVIPWSMIADRIGRVKAMSVSVIASSVL
                     GLLTPFAPSFELLLAGRLLEGLV"
     gene            557..831
                     /locus_tag="VGP27_25715"
                     /pseudo
     CDS             557..831
                     /locus_tag="VGP27_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305138.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1686..2447)
                     /locus_tag="VGP27_25720"
     CDS             complement(1686..2447)
                     /locus_tag="VGP27_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854734.1"
                     /translation="MKDILIGVAVFAIIFGGAMFGMFLGKILPDQHVGTESRDAIRIV
                     MAMLATLSAVVLGLLTGSSIGSLGEREGELRSAGVQFIMLDRTLAEYGPETAQPRALL
                     KQLLEERISQIWPEEDGAVSLTALSSGAGIVLVQRDLFALSPQTEQQRWLRSNALQIT
                     NTIAESRWTTIEQIGSRFPWAFFIVVVAWLTIIFASFGLYAPRNASVIAALLVAALTL
                     AGPIFMMLEMDQPYGGLVKIPSTSLRVALDQLGRS"
     gene            2407..4449
                     /locus_tag="VGP27_25725"
     CDS             2407..4449
                     /locus_tag="VGP27_25725"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV7854735.1"
                     /translation="MIAKTATPIKMSFIRFSACRQPFRSAHLLSQSGRMLHRATRCHS
                     LNRTPCRFPFAATYERLRSPHGAPACCAQRQQYLRPRVSMMSPRSFRGFIADFRWALL
                     GFAAVIAFTLGWLGYTEYLNEQYTEGVIKHPAGATDIAYNTFKLFLMGSPGTTGLPVK
                     IEIARILAPVVSGWAALSGLALLFHDRFQQLRIPLMRGHVIICGLGYVGSLFGARLRQ
                     AGYQVVVVELDPANSSLQACRSWRSPVILGDAQLEATLREAGLQRAARLLAVCPDDAV
                     NTEIVAVARSSRTGRRRGQLRSLARIENPELCRLLRVQEANSKDGAPSSLDFFNIDEI
                     GARLCLDDFPLAAAGENGRPHILVSRLDAMGTWLVKHAAWGWFTDRTDETPLWVTVVD
                     DQARDRLRGLLDQYPAIESVCRFIESSMSVRDLHRLDAMQAEAGAPPITRAYVSAYRD
                     EDAIEAMLRLRHDLDPKVPIVVALSRTDGVARLITDATASGELTNVSVFPALERTCTA
                     EFVAGGSFERIAVAIHDRWRARQLAVGKDAPPWSDLDESRKESNRAQARHMPVKLRSI
                     GCTIAPLHDISAPSFEFTNEEFEMLAIAEHKRWVTERLESGWRLGPKDIDQKTTPYLI
                     PFDELPDDIADLDRDAVRQIPDALALVDLKAIRSATTSGRVGEPTLIGDGGVGLPR"
     gene            complement(4446..8717)
                     /locus_tag="VGP27_25730"
     CDS             complement(4446..8717)
                     /locus_tag="VGP27_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEV7854736.1"
                     /translation="MSRIFLSHSNRDAREALALKRWLVGQEPPLATDIFLDIDPEVGL
                     RPGERWKDRLQQASAKCEAVVCLLSGNWEASHECRTEYRVAENLNKQILCARLEPSAG
                     DDLTSEWQRCDLFGESDMTSIDVSGGPPVMFATAGLYRLRDAIRGTGISAHSFVWPPP
                     DDVGRAPYRGWEPFEQADAAVFFGRDAPIVHGLDELRGMRLAETKSLLVVLGPSGSGK
                     SSFLRAGLLPRLAREDRRFTLLGVVRPGRNALTGDSGLAAGIWHARDKLGLSTPSLGE
                     LKAACTDDIERLRELLLEIQQAAANRLPDPVRGDEKPTPPTLILPLDQAEELFSADAG
                     VQGAELMSLIRRLTVRTDLQPLGLIVVATIRTDRYEVMQTHPELAGAESRVFDNLKPM
                     PPTQFKEVITGPAARASEGGRRLAISPDLVEKLLGDAADGADTLPMLSLTLARLYADY
                     GSTGELTVEQYEAMGGMRRVVETEINEVLAADPDRRRHQLDCLRTAFIPWLATVNPDN
                     DQPLRRMARYDDLPEESRPLIDALVAKRLLLKDRRDGQVVVEVALESLLRQWDDLADW
                     LREQRQELQVADDVERADTAWRSNGKDPAWLLAGSRLADAEKVIGSDTFTQRMNPVRE
                     YLDASRQIENERVAGEEERQHAELRNAQERQAEAEAHATDLRKHATDLRKRSRILRAV
                     VAMTAVVAVVAVIGLVYAMTARGQAQDRFRQATGVRLAAETDGILDGSRPGSDARALL
                     ELLAANALSPNTFEQQLYSAAVKTYDTLRLIDTAQAIPGAAFSPAAAFSPRGDRFVTA
                     GDHGTLRMWDTHTGQQVGKDFVGHSKWVNDAVFSPDGHRLATTSDDRTVRLWNADTGE
                     PIGRPMTGHEDEVWGVAYSPDGLMIATGSVDTNVRLWNANTGQPIGKPLDHPAKVVCV
                     EFSRDGRRIVTGGYDGAIRIWDVGTGRQIGPPHKVREEDKTEIIMDVAFSPDGRRLAS
                     AGAHGDVQIWDGQTGLPIGGPLAGHGTQVTNVTFAPDGHRLATGGSDGAVRLWDVDTG
                     QLLGAPMLGHKSAVFTLSFNPEGTQLLSGSQDGTIRLWDVGIRRPLFGHIGKVSSVAV
                     SPDGHRIASAGDDGTVRFWNADNGRPMTKPLVASGQALTSVVFSPDGRRLATAGADGT
                     FRMWDADTGTPVGAPTNPGQGPLTSVAFSPDGRRLAIGGGDPTVGVTGDHDGTVGMWD
                     LETNSPIGMPHAHGELVHSVAFSPDGNRLVSGGVEAGYNGTVRLWDTNTGQLIRAVPI
                     GGADVVVFAVAYRPDGRRLAAAGGDGVIQRLDAGSLDKVGEPMQAHEDTVVALAYSPD
                     GRTLASASLDETIRVWDVESGKQAADALTGHTDDVDSVVFTPDGQRLVSGSTDGTVRV
                     WPAVAGPKDLCDKLTANMSYNQWKEWVSPDIDYIKVCPDLPIRPD"
     gene            8824..9561
                     /locus_tag="VGP27_25735"
     CDS             8824..9561
                     /locus_tag="VGP27_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV7854737.1"
                     /translation="MTSKPRPGEALVPNKAQPHHGSVANKLNWLRAAVLGANDGIVST
                     AGIVIGVAAATAERGPVLTAGIAGLAAGALSMAVGEYVSVSTQRDTERALLYTEGQEL
                     KLQPDAEMDELAGLYEARGLSPQTARQAATELTAFDPLAAHAELELRIDPENLTNPWL
                     AAISSALSFTIGALLPLIAILLPPPHARIPVTVAAVLLALAITGWVAARLGGAQPLRP
                     ILRVTVGGAMAMAITFAIGHLVGATIG"
     gene            9881..11452
                     /locus_tag="VGP27_25740"
     CDS             9881..11452
                     /locus_tag="VGP27_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854738.1"
                     /translation="MALVIVLGAGSLGTSSPSIADTGAVDAYSDQLAEAADTPALGWA
                     DCDDGFVCATAKVPLNYNRPDAEQIELAVIKLPATDPQNRIGTLFINFGGPGQSGVDR
                     LRGRARWPWLFSEELRSRFDLVSWDQRGVARSAAVRCFPDAPQQWQFLVPSPGLPMDA
                     QEEQALFTWSEDFAGRCEQQAGPILNHVSSANSARDLELLRRAVGDSSLTYHGISYGT
                     QLGAVYANLFPGRVRAMALDGSIDFEGNANGHGAEGKTLPLNTRQDIATGTAAAFDQF
                     LLDCSAAGQRCAFSEGDPKAKWAALAQRSRMSPVLVYGHAWTYPEIVVATLAKPSAYP
                     QLADLLQQFSETGTAAPDLIDAITGSDPPPADVDRSYLGNREEAYDAIQCSDSTVPTD
                     PAAYRRAALAADEASPEFGRISVFDVMPCAFWQGHDADRYMGPWNRRTSAPILVLNTR
                     NDPATPLAGAYAGAAQLHEAQVVVTEGSGHTSMYVASTCAERVKRDYLFSGLLPPAGT
                     GCSRDQSPFDQAPPS"
     gene            complement(11468..12526)
                     /locus_tag="VGP27_25745"
     CDS             complement(11468..12526)
                     /locus_tag="VGP27_25745"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7854739.1"
                     /translation="MNDVKSEPLRVALVAPPYYEVPPKDYGGTEIVIADLANALVRRG
                     HKILVLGAGRSTTDAQFVAISPEAVPERLGQVRPEIVHAIEVRRTIARIAHRGGLDIV
                     HDHTMSGGLNASSYRSLGVKAVSTVHATIDADMHRYYLAVGRDLGLVAISHRQRMMAP
                     DLNWVSTVRHGLSVSDWPFRSTKDDFALFLGRFCPEKAPHLALDAAHAAGVPTVLAGA
                     FVELTDRAYFEQQVRPRLTDNDTVIGPLGGEAKRQLMAAARCLLFPIRWEEPFGLVMI
                     EAMVTGTPVLALRAGSVSEVVVDGETGYVCDDPSDLGAALEAIGDIDPYACRSHVEKY
                     FGLERMVAEYEDVYASLQ"
     gene            complement(12519..13874)
                     /locus_tag="VGP27_25750"
     CDS             complement(12519..13874)
                     /locus_tag="VGP27_25750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854740.1"
                     /translation="MGLFDDGVVADLGYSWRSDKAKFFPNGPDSYRYPQWLEPTINQT
                     VADSHWPILSVELEDIRRCVAAFEELEEPKLKSIVDGIGNLEKLYFHHFSSWLEHHEP
                     DWVFAINLTLPHAVSATSALYMAADLYYKNHPGGLLIWDHDLCGSNGRWDAEVNRRFY
                     PTVPNNLTPLPPEAPHIKWIVVSQALAREAETYETTVRPQVVANVLPTVPSGIEPHHR
                     DFARQFDLAPGRPILLNPVRIYRVKGVSQALRFHAEVVDKCSRRLLPPPYLLIFGSLD
                     EDPDYADELIALRDELSIEEYVRFLGGVPLETMREDNGDWRLDEVDLLRLARATNGGV
                     VFTPCVNDFETVGLGPGLAGAAEIPAVSTRYNAYDEVYGAQAFACTTFAPDGTGMTEA
                     AAKFVDVLSNFARRDGTLIAELKRNRMVVESLFPAAPWRTLLGSMNGNIDSPAASRRC
                     E"
     gene            complement(14498..>14974)
                     /locus_tag="VGP27_25755"
     CDS             complement(14498..>14974)
                     /locus_tag="VGP27_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7854741.1"
                     /translation="NRLNPIHEGTHGIQSLDLLGRKVTMRGGAGLAALGDAIGRSVAE
                     AAALNGEPAEQAAQLDAAWQRLVEVTTAMFGSGDFEAALANSVIYLEAFGHIVVAWMW
                     LQQVVAAHGRDGDFYDGKRHAARYFFRYELPRTTPQLDLLQSLDRTTLEMQDSWFG"
BASE COUNT         2749 a         4772 c         4570 g         2883 t
ORIGIN      
        1 cacgagtcct tccagcagcc gtccggcaag gagtagctcg aagctcggcg caaacggcgt
       61 aagcaggccc aggacgcttg acgcgatgac cgagacggac atcgccttca cccggccgat
      121 gcggtcggcg atcatcgacc aggggatgac accgatggcc agaccgatcg tgcaggccga
      181 caccatgagc gccgcactgg ccgcgcccac gcccaggtcg gctgagatct gcggcagaac
      241 gccctgcggc gaatagagct gggcgaacgt ggctatcccc gcgcagaaca atgctgccag
      301 cagacggcgg tattcagtcg acccccggag gtggccagcc gggggttgtt tgcggctgag
      361 ggagatggaa tccatggcac accttctgtg aggtctcggg tcaaggcgat cgattgctcc
      421 aagctatgtg gtcgccgacg tcgtggctcg cttgtgcgcg caagccgcgc aatgcgcgat
      481 cttcatcttt tgcgcattgt gctcaccaac gccgggcgtg cgcggctggt ccccgcccct
      541 gagcgccggc aatcacgcat tgctgcgcct tctcgtgggt ccgcttgccg atgcacaacg
      601 aatgcggtat ccgaagttcc ttgctgccaa cgcttttggg ggtctctcct ggcgggcggc
      661 acaaccgcgg tcgtctattt cgctgtgctc gtcggaatcg cgctcacgtt gctgctgcgg
      721 ggaacgggcc gcaccgctga tccgcgatct ggacgaggcg catacctggg actgcctgcg
      781 cacagactag cgctcaaatt tagacgctca gcaccacggc gcttggagta atttctgaat
      841 gtctctgggc cgcaatcacg ctcaaagtcg atcgtccccg tccggcgcaa tggaaagggc
      901 cgctattcgt cgctactacc gacacgatta tccggttagt cgactttcga caatgtgaac
      961 tcgctagtcg gaccacggga gccgtcaggt tcggtgctgc cacacgtacc ggggcccgct
     1021 tggctgaacg cggcgccggc gagtgtttcc gcaccccatg aatatatggt cgttcccgca
     1081 gcacggctgt tgtcgccgca agtgaaggcg tcgggccgcg cagccgtcat gttccagcga
     1141 tcagccgcca actgcgcttg cccgctgtag ggcgcgtttc cagccgacga gtttgtcgcc
     1201 gcgacatcgg cgcaacccgg gccacacggt gtgatcgtcc atctggttgt gtttccggat
     1261 cccaccgtga aggtgtaagt gccgctcaac tcgctggcgg gatgggccgc gtcggaagca
     1321 ctgggcgcgg tgggtgtagt cggaccgcca gcccagccgg gactcgctgt cccgatagct
     1381 accgcaataa aggcggccac tgcaccaagt acgcgagcga taatcatgtt ttcctctcaa
     1441 tggctccgag acgttaccgc tcagctgtta taggcagtat cgcagtagta tcgcagtcgt
     1501 ttcaccgcag tatcagccat tcaggtaccc tctctccgac cgccgtaaga cttgtacggt
     1561 cacgtctatc gctatggcgt gcttcgcctc tcgccgggta acgccgacgg cgtttcatcg
     1621 cggcgagatt gcgcgttgta aggccaatcg atcacgccgc cagcaggttt gctttgagag
     1681 tctgtttacg atcgacccag ctgatccaag gcgacgcgaa gtgacgtgct ggggattttg
     1741 accaacccac cataaggctg atccatttca agcatcataa aaatcgggcc ggcaagggta
     1801 agcgcagcga ccagcaatgc tgcgatcacg ctggcattgc gaggcgcgta gagcccaaaa
     1861 ctggcgaaga tgatcgtcag ccaggccacg acgacaatga agaatgccca cgggaacctg
     1921 ctgccgatct gttcgatggt cgtccatcgc gactccgcta tcgtattggt gatctgcagc
     1981 gcattggacc ggagccaccg ctgttgttcc gtctgcggcg acagggcgaa gagatcccgc
     2041 tggaccagga cgataccggc cccgctgctg agtgccgtga gcgataccgc accgtcttct
     2101 tccggccaga tctgacttat tctttcttca agcaattgct tgagtagagc ccgtggttga
     2161 gctgtctccg gcccgtattc ggccaaggtc cggtcgagca tgatgaattg cacgcctgca
     2221 ctgcgcagtt caccttctct ctcgccgagc gagccaatcg aggagccggt gaggaggccg
     2281 agcaccactg ctgacaatgt tgccagcatg gccattacga tcctgatagc gtcgcgcgat
     2341 tcggtgccca catgctgatc aggaaggatt ttcccgagga acatcccaaa cattgcgccg
     2401 ccgaatatga tcgcgaagac agcgacgccg atcaaaatgt ctttcatccg cttctccgct
     2461 tgtcggcagc cgttccgatc ggcccacctc ttgtctcaat ccggtcgaat gctacatcga
     2521 gccacacgtt gtcattcgct gaataggacg ccatgtcgct tccccttcgc agcaacatat
     2581 gaacggttac gctcccccca tggcgctccg gcatgctgcg cgcagagaca gcagtactta
     2641 cgtcctcggg tatcgatgat gtccccaagg tcctttcgag gcttcatcgc tgactttcga
     2701 tgggccttgc tcggtttcgc cgccgtgatc gcgttcaccc ttggctggct cggatatacg
     2761 gaatacctga acgagcagta caccgagggg gtaattaagc accctgcggg ggcgaccgac
     2821 attgcctaca acaccttcaa gctgttcttg atgggctcac ctggcacgac cgggctaccg
     2881 gtcaagatcg agatcgctcg gatcctcgcc ccggtcgtgt ccggttgggc cgcgctgagc
     2941 gggctggccc tgttgtttca cgatcggttc caacagctcc gcatcccgct gatgcggggc
     3001 cacgtcataa tctgcggact cggctatgtc ggcagcttgt tcggggcacg tcttcgccag
     3061 gccggctatc aggtcgtcgt tgtcgaattg gaccccgcga actcgtccct gcaggcatgt
     3121 cgcagttggc gcagcccagt cattttgggt gacgcgcagc tggaagccac gctgcgtgag
     3181 gcggggctgc aacgtgcggc ccgacttctc gcggtgtgcc cggacgatgc ggtgaacacc
     3241 gagatcgtcg ccgtcgcgcg gagttctagg accggccgcc gacgcggtca gctgcgttct
     3301 ctggctcgaa tcgaaaaccc ggagctgtgc cggttgttgc gcgtccagga ggcaaactcc
     3361 aaggacggcg ccccctcgtc actggacttc ttcaacatcg acgagatcgg cgcgcggctg
     3421 tgcctcgacg atttccccct tgcggccgct ggggagaacg gacggccgca cattctcgtc
     3481 agccgccttg atgcgatggg gacctggttg gtgaaacacg cggcctgggg ctggtttaca
     3541 gaccgaactg acgaaacgcc actgtgggta acggttgtcg acgaccaggc ccgggaccgc
     3601 cttcgtgggc tcctcgacca atacccggcc attgaatcgg tgtgccgatt catcgaatcg
     3661 tcgatgtcgg tgcgtgatct gcatcgactc gacgcgatgc aagccgaggc gggagcgccc
     3721 cccataacgc gcgcctacgt gagcgcatat cgcgacgaag acgcgattga ggccatgctc
     3781 agattgcgtc acgaccttga tccgaaggtg ccgatcgtcg tcgcgttgtc acgcacggat
     3841 ggggtggccc ggctcatcac cgacgcgact gccagcggtg aactgacgaa cgtcagcgtg
     3901 ttcccggcgc tggagcgcac gtgcacggcc gaatttgtag ccggcgggtc attcgagcgg
     3961 atcgcggtgg ccattcacga ccgttggcgc gcaaggcaac tcgccgttgg caaggacgcg
     4021 ccgccgtggt cagatctgga cgagtcgcgc aaggaatcca atcgagccca agcccgccac
     4081 atgccagtca agctgcgcag catcgggtgc acgatcgctc cgctgcatga cataagtgca
     4141 ccatcgttcg aattcaccaa cgaggagttc gagatgcttg cgatcgccga gcacaaacga
     4201 tgggtaaccg aacgactgga gtccggctgg cggctggggc ccaaagatat tgatcagaag
     4261 accacgcctt atcttattcc gttcgacgag ctgccggacg acatcgccga cctcgatcgc
     4321 gacgcggtgc gtcagatccc tgacgcacta gccttggtcg atctcaaagc catccggtcc
     4381 gccacgacct cgggccgtgt tggagagccg acgctcatag gcgatggtgg ggtaggcctt
     4441 cctcgctagt cgggacggat gggcagatcc gggcagacct tgatgtagtc gatgtccggt
     4501 gatacccatt ccttccactg gttgtagctc atattcgcgg tgagtttgtc gcacagatct
     4561 ttcggacctg caacggccgg ccagacccgg acagtaccgt ccgtgctgcc cgaaactagg
     4621 cgctggccgt cgggggtgaa cacaacactg tccacgtcgt cggtgtgtcc cgtcaacgca
     4681 tcagctgcct gcttgcccga ctcgacgtcc cacacccgaa tggtttcgtc gagactcgcg
     4741 gacgcgaggg tgcggccgtc cgggctgtag gccaacgcaa cgaccgtgtc ctcatgggcc
     4801 tgcatcggct cgccgacctt gtcgaggctg cccgcatcca gccgctgaat gacgccgtcg
     4861 ccgccggcgg ccgcgaggcg gcgcccgtcg gggcggtagg ccactgcgaa cacaacgacg
     4921 tccgcgcccc cgatcgggac tgcccgtatc aattgacctg tgttggtgtc ccacaacctc
     4981 actgtgccgt tatagcccgc ctccacgccg ccggagacta agcggtttcc atctggactg
     5041 aacgcgacac tgtgtaccag ttcgccgtgg gcgtgcggca taccgatggg actgttcgtt
     5101 tcgagatccc acatcccgac ggtgccgtcg tggtcgccag tcactccaac cgtggggtct
     5161 ccgccgccga tggccagacg gcgtccatcg ggactgaacg ccacgctggt cagtggtccc
     5221 tgcccgggat tggtcggggc gccaacgggt gttccggtgt ccgcgtccca catccggaag
     5281 gtgccgtcgg cgccggcggt ggcgaggcgc cgtccgtccg ggctgaaaac tacgctggtc
     5341 agtgcctgcc cgctggccac gagcggcttg gtcatcggac ggccgttgtc ggcgttccag
     5401 aatcgcaccg tgccgtcatc accagccgaa gcgatgcgat gtccgtcagg gctgaccgcc
     5461 acgcttgaca cttttccgat gtggccgaag agcggacgac gtatgccgac gtcccacaat
     5521 cggatcgttc cgtcctgcga tcccgacagt agttgggttc cttcaggatt gaacgacaga
     5581 gtgaataccg cgcttttgtg gcccagcatg ggggcaccga gcaactgccc ggtgtcgacg
     5641 tcccatagcc gcaccgcacc gtcactacct ccggtagcaa gccgatgccc gtccggggcg
     5701 aacgtcacgt tcgtcacctg ggtcccgtgc ccggccaggg gaccgccgat cggcaggccg
     5761 gtttgcccgt cccagatctg cacgtccccg tgggcacccg ctgaagcgag tctgcggccg
     5821 tcggggctga acgccacgtc catgatgatt tccgtcttgt cttcctcgcg gaccttgtgt
     5881 ggaggaccta tctgccgccc ggtgccgacg tcccagattc ggatagcccc gtcgtaaccc
     5941 ccggtgacga tccgccgtcc gtcgcggctg aattccacgc agacgacctt ggcgggatgg
     6001 tcaagcggtt tcccgatggg ttggccggtg ttcgcgttcc agagccgcac gttggtgtcc
     6061 acactgcccg tggcgatcat gagtccgtcg gggctgtatg ccacccccca cacctcgtct
     6121 tcatggcctg tcatcggccg gccgatcggt tctccggtgt ccgcgttcca cagccgtacg
     6181 gtgcgatcgt cactcgtggt cgcgagtcgg tgaccgtcgg gactgaacac cgcatcgttg
     6241 acccacttac tgtgcccgac gaagtctttg cccacctgtt gaccggtgtg ggtgtcccac
     6301 atgcgcagag ttccatgatc gcctgcagtg acgaagcggt caccgcgagg gctgaacgcg
     6361 gcagcggggc tgaaggcggc cccggggatc gcctgtgcgg tgtcgatgag tctgagggtg
     6421 tcgtaggtct tgaccgccgc ggagtacagc tgttgctcaa acgtgttcgg agagagcgcg
     6481 ttcgcggcaa ggagctcaag cagggccctg gcgtcactgc ccggcctgct gccgtccaag
     6541 atgccgtccg tctcggcggc gagccgcacg cccgtcgcct ggcggaaccg gtcttgggcc
     6601 tggccacgag cggtcatcgc gtagacgagc ccgataacgg cgaccacggc gaccacggcc
     6661 gtcattgcaa cgacggcgcg caagatccgc gatcgcttgc gcaggtcggt cgcgtgcttg
     6721 cgcaagtcgg ttgcgtgcgc ttcggcctct gcttgtcgtt cttgggcatt gcgtaattcg
     6781 gcgtgctggc gttcttcttc cccggcgacc cgctcgttct cgatttgtcg agaagcgtcg
     6841 aggtattcgc gaacaggatt catccgttgc gtgaaagtgt ctgagccaat gactttttcg
     6901 gcgtcagcaa gacgagaacc ggcgagaagc caggctggat ctttgccgtt gcttcgccat
     6961 gctgtgtcgg ctcgctcgac gtcgtcggcg acctggagtt cctggcgttg ctcgcgaagc
     7021 cagtcggcca aatcatccca ctggcgcagc aggctttcca gggcgacctc gaccacgact
     7081 tggccgtcgc gccggtcctt cagtagcagc cgcttggcga ccagtgcgtc gatcagtggt
     7141 ctgctttcct caggtaggtc gtcatagcgg gccatccgac gcaacggctg atcattatcg
     7201 gggttcactg tggccagcca cggaatgaaa gccgtccgca gacaatcgag ttgatggcgc
     7261 cgtcggtccg ggtccgcggc gagaacttca ttgatctcgg tctcgacgac acggcgcatc
     7321 ccacccatcg cctcgtactg ctcgacggtc aactcacccg tggagccgta gtcggcatac
     7381 agtcgcgcca gcgtcaacga caacattggc agtgtgtcgg cgccgtcggc ggcgtcaccg
     7441 aggagcttct ccaccagatc gggtgaaatc gcgaggcggc ggcccccttc gcttgcccgc
     7501 gcggccgggc cggtgatcac ctccttgaac tgggtcggcg gcattggctt gagattgtcg
     7561 aaaacccttg actccgcgcc ggccagttcc ggatgggtct gcatgacctc atatcggtcg
     7621 gtccggatgg tggcgaccac gatcaggccg agcggttgca gatcggttcg caccgtcagc
     7681 cgccgaatca gcgacatcag ctcggcccct tggactccgg cgtcagcgga gaacaactcc
     7741 tcggcttggt ccaacggcag tatcagcgtg gggggagtcg gtttctcatc gccgcgaacc
     7801 ggatccggta accgattcgc cgcagcttgc tgtatttcga gcagcagctc gcgaagtcgc
     7861 tcgatgtcgt ctgtgcacgc ggccttgagt tcgccgaggc ttggcgtcga caaccccagc
     7921 ttgtctcgtg catgccaaat ccccgctgcc aggccggagt ccccggtgag cgcgttgcga
     7981 ccgggtcgca cgacgcctaa cagtgtgaaa cgacggtctt cgcgggccag gcgtggcagt
     8041 aaacccgcgc gaagaaacga cgacttacct gagccagagg ggcccaatac gactaacagc
     8101 gacttcgtct cggccagcct catgccgcgg agctcgtcaa ggccatgcac gatcggcgca
     8161 tcccggccga agaacacggc cgcatcggcc tgctcgaatg gttcccaacc gcgatatgga
     8221 gcccgaccga cgtcgtctgg cggcggccat acgaacgaat gcgcgctgat acctgtcccc
     8281 cggatggcat ctcgtaaccg gtacaagcca gcggtggcga acatcaccgg tggtccgccg
     8341 ctcacatcga tcgaggtcat gtcgctctcg ccgaacaggt cgcaccgctg ccactccgac
     8401 gtcaagtcgt caccggcgga aggctcgagg cgggcacaca gaatctgctt gttcaggttc
     8461 tccgcgacgc gatattcagt tctgcactca tgggaggcct cccagttgcc agacagcagg
     8521 cagaccacgg cttcgcattt ggcactcgcc tgctggagtc tgtccttcca tcgctctccc
     8581 ggtcgcagcc ccacttcggg atcgatgtcg aggaatatgt cagtcgcgag tggcggctcc
     8641 tggcctacca gccagcgctt gagagccagc gcctcgcgcg catcacgatt tgaatggctg
     8701 aggaaaattc gcgacatatc atccttgggg ctggttagtt cgcaggttaa accccgtttc
     8761 cgaatcataa agtcgctttg ccgtaaatca tcgtcagcgc cagcgggcga ttaacctcac
     8821 tagatgacat caaagccccg acccggtgag gcactggtgc cgaacaaggc gcagccacac
     8881 cacgggtcgg tcgccaacaa actcaactgg ctacgtgccg ccgtgttagg tgccaacgac
     8941 gggatcgtct cgacggccgg cattgtcatc ggcgtcgccg cggccaccgc cgaacggggg
     9001 ccggtgctga ccgccggtat cgctgggctg gcagccggag cgctgtcgat ggcggttggc
     9061 gagtatgtgt ctgtcagcac gcaacgcgac accgaacggg cactgctcta cacggaaggc
     9121 caagagctaa aactgcagcc cgatgccgaa atggacgaat tggcaggcct atacgaggca
     9181 agaggtctct ctccgcagac cgcgcgccaa gccgctaccg agttgaccgc ttttgacccg
     9241 ctcgccgccc acgcggagct cgagctgcgg atcgatcccg agaacctgac aaatccctgg
     9301 ttggcggcga tctcgtccgc gctgtcgttc accatcggcg cgctcttgcc gctcatcgca
     9361 attcttctcc cgcccccgca cgcccggatc ccggtcacgg tcgccgcggt cctgctcgcg
     9421 ctggcgatca ccggatgggt ggccgcccgg ctcggcggcg cacaacctct acgaccgata
     9481 cttcgggtaa ccgtaggcgg cgcaatggcc atggccatca cattcgccat cggccatctt
     9541 gtcggcgcga cgatcggata gcaaccttcg cgtggcaaac acacatattt gtcgcggtgg
     9601 tcagttacgc tgcgcttgaa agagggcaca aacgccgaca gcaagactct tcgaccggat
     9661 gctcggagtg atcagtcggg ggaggcgcag atgctcagcg gcgccgtttt cacggcgccg
     9721 gctacatcga cgtgctggcc gtaatcagtc ccacctcaat tgtgctgctg cggcagcagg
     9781 ccaatgccgg agccaaggaa ctgacggagc aactgtgaag cggccaaggc gatttgccca
     9841 cggatcggcg gtgcgctgct cgtggcgtcg ccttgcattc atggcgttgg tgatcgttct
     9901 cggcgctggt tcgttgggca cgtcttctcc gtcgatcgct gacaccggcg ccgtcgacgc
     9961 ctacagtgat cagctggccg aagccgccga cacgcccgct ttgggctggg ccgactgcga
    10021 tgacgggttt gtctgcgcga cagcaaaagt ccccctgaac tacaaccggc cagacgccga
    10081 gcagatcgag ctagcggtga tcaaactgcc cgcgaccgac ccgcagaacc gaatcggaac
    10141 gctgttcatc aatttcgggg gtcccggtca gtccggcgtt gaccggcttc gcggacgtgc
    10201 gaggtggccg tggttgttct ccgaggaact gcgatcgcga ttcgatctcg tttcttggga
    10261 tcagcgggga gtggcgcgca gcgccgcagt gcgctgcttt cccgatgcgc cccagcaatg
    10321 gcagtttctc gtaccgagtc cggggctgcc catggacgcc caggaagagc aagcactctt
    10381 cacatggtca gaggacttcg ccggtcggtg cgagcagcag gccggcccga tcctgaacca
    10441 cgtctcctcc gccaactcag cacgggatct cgagttgttg cgccgcgccg tcggtgattc
    10501 gtcattgacc tatcacggca tctcgtacgg cacccaactc ggtgcggtct acgccaatct
    10561 ctttccggga cgcgtgcgtg caatggcgtt ggacggatcg atcgacttcg aaggcaacgc
    10621 caacggtcat ggtgcagaag gaaaaacgct cccgctcaac acccggcagg atatcgctac
    10681 cggcacggcc gcggcgttcg accagttcct gctggactgt tcggcggccg gccagcgctg
    10741 cgcgttctcc gaaggtgatc ccaaagccaa gtgggcggca cttgcccaac gttcgcgcat
    10801 gtcacctgtt ctggtctacg gccacgcgtg gacctaccca gagatcgttg tcgcgacact
    10861 ggcgaagccc tccgcctacc ctcaactagc cgacctgctg caacagtttt ccgaaacagg
    10921 taccgcagca ccagatctca tcgacgcgat cacgggcagt gacccgcccc cggctgatgt
    10981 cgacagatcg tatttgggca atcgtgaaga agcgtacgac gcgatccagt gcagtgacag
    11041 cactgtgccg accgaccctg ccgcgtaccg ccgagccgcg ctagcggcgg acgaggcgtc
    11101 accggagttc ggtcgcatct cggtgttcga cgtgatgccg tgcgcgttct ggcagggcca
    11161 cgacgccgac cgctacatgg gtccctggaa ccggcgcaca tccgcgccga ttctggtgct
    11221 gaacacgagg aacgaccccg ccactccact ggcgggcgca tatgccggtg cagcccagtt
    11281 acatgaggct caagtggtgg taaccgaggg gtcaggacat acgagtatgt atgttgcgag
    11341 cacctgcgca gagcgcgtca aacgcgatta tctgttctcc ggcctgctgc cgcccgcagg
    11401 taccggctgc agccgcgacc agtcaccgtt cgatcaggcg ccgccgtcgt agagtcagac
    11461 atgccgtcta ctgaagggac gcatagacgt cctcgtactc ggcaaccatg cgctccaggc
    11521 cgaagtattt ttcgacatga gatcgacatg cgtacggatc gatatcgccg atcgcttcca
    11581 aggcggctcc cagatctgac gggtcgtcgc agacgtagcc tgtttcgccg tcgacgacga
    11641 cctcactgac agagccagcc cgcagggcca ggaccggtgt gccggtcacc atcgcctcga
    11701 tcatcaccag gccgaatggc tcttcccagc ggatcggaaa caaaaggcac cgcgcagcag
    11761 ccatcagctg gcgctttgct tcgccgccga ggggtccgat taccgtgtcg ttgtcggtta
    11821 aacgtggccg gacttgctgc tcaaaatagg cgcggtcggt caactcgacg aaagcgcccg
    11881 ccagaaccgt ggggacgcct gcggcgtgcg ccgcatccaa cgccaagtgt ggcgctttct
    11941 ctggacagaa tcgacccaga aacagtgcga agtcatcctt cgtcgaccga aacggccaat
    12001 cgctgacaga aagcccgtgg cgtactgtgc ttacccagtt caggtccggc gccatcatcc
    12061 gttgacgatg actaattgca acgaggccga gatcgcgacc gacagccagg tagtaccggt
    12121 gcatgtccgc atcaatcgtc gcatgcacgg tcgacacggc cttcacgcca aggctgcggt
    12181 agcttgatgc gttaagtcct ccggacatgg tgtggtcgtg aacgatatcc aggccgcccc
    12241 ggtgggcgat tcgcgcgatc gtccgacgca cttctatggc gtgcacgatc tccgggcgaa
    12301 cctggccaag gcgttcggga acagcctcag gcgaaattgc aacaaactgc gcgtctgtag
    12361 tagaccgtcc cgcgcccaga acgaggattt tgtggccgcg ccgtacaaga gcattggcca
    12421 gatccgctat gacgatctcg gttccgccgt aatccttggg aggaacctca tagtatggcg
    12481 gcgcgacaag ggcgactctc agcggttccg acttcacatc attcacaccg gcgggacgca
    12541 gccggactgt caatattgcc gttcatcgag ccaagcagag tcctccacgg ggcagccgga
    12601 aatagcgact caacgaccat tcggtttcgc ttcagctctg cgatcagcgt tccatctcgc
    12661 cgagcaaaat tggacagcac atcgacgaat tttgccgccg cttccgtcat gccagttccg
    12721 tcgggcgcga acgtagtgca ggcgaaggct tgcgcaccgt agacctcatc ataggcgttg
    12781 tatcgggtag agacggcggg aatttcggcg gcacctgcca agccagggcc aaggccaacc
    12841 gtctcgaagt cgttcacgca cggcgtgaag acaactccac cgttcgtcgc ccgcgccaga
    12901 cgcaaaaggt cgacttcgtc taaccgccag tccccgttgt cttctcgcat cgtctcaaga
    12961 gggacaccgc cgagaaatcg aacgtattcc tctatagaga gctcatcgcg gagtgcgatt
    13021 agttcatccg cgtaatccgg atcctcatcg aggcttccga agatgagaag atacgggggc
    13081 ggaagcaaac ggcgggaaca tttatcgacg acttctgcgt gaaatcgcaa tgcctgactt
    13141 acgcccttta cccggtatat tcgcaccgga ttcaaaagta ttggccgtcc gggggccaag
    13201 tcgaactgtc gcgcaaaatc acggtggtgt ggttcgatcc ccgatgggac cgtgggcagc
    13261 acattggcga cgacctgtgg gcgcactgtg gtctcatagg tttcagcctc ccgggcaagc
    13321 gcctgcgaaa cgactatcca cttgatatga ggcgcttcag gggggagggg cgtcaagttg
    13381 ttggggacag tcggataaaa ccgacgatta acctcagcgt cccagcgccc attcgatccg
    13441 cacaggtcgt gatcccaaat cagcagtcca cccgggtggt ttttgtaata aaggtctgca
    13501 gccatgtata gcgctgacgt cgcactcacc gcatgtggaa gggtgagatt gatagcgaag
    13561 acccaatctg gctcatggtg ctccagccac gacgaaaaat ggtgaaaata cagcttctcc
    13621 agatttccga ttccgtcgac gattgacttc agcttcggtt cctcgagctc ttcgaaggcg
    13681 gcgacacatc gccgaatatc ctccagctcg accgacaaaa taggccaatg cgagtccgcg
    13741 accgtttgat tgatcgttgg ttcgagccac tgtggatacc gatatgaatc cgggccgttt
    13801 ggaaagaatt tggctttgtc ggagcgccaa gagtatccaa gatcggccac gactccgtcg
    13861 tcgaaaagcc ccatcgacct accgatatga aaaacattgc ggaagaccgt tagcaagcca
    13921 gagaccggta aaccgtcccc agagatgagt ccgatcctca acccgactcg cttccgatac
    13981 gcgctgggat tacttccgac cttgcgagac ttctttaacg acccgccgta ctcgaagaca
    14041 tttcggaatg gaatgctaca cacctcgagg cgagcttgag cgtcggcgcg cgtggccgcc
    14101 cggcgccttc catccgtggt agctcgaggg cagacaccag tggcctgtgc ctttatggat
    14161 tgatgcaacc gcgacctggc gagtctggcc ggccgacacc gtccccgggc tgccctatct
    14221 ggccgggctg caagggaccg tcgtcctcgt cgttgcggcg gtgctcgtgg gcatcgtgct
    14281 tatcggcacc ggtgtagatc gtggggctgc tgtccggcgg accgcccctt cggcgcgcgt
    14341 tgcgccaact cctaatcgga tacggcgccg ccgcagtcac atatctgttg agtctaatgc
    14401 tgggcacagg attcggttaa gcgataccgc cgtgctcgca tccgcgcttt tgtgtgtggc
    14461 ttgagattgc ggccagggca aacttcaagc gacgaggtca tccgaaccag gagtcctgca
    14521 tctccaacgt cgtgcggtcg agcgactgca gaaggtccaa ttgcggtgtc gttctcggca
    14581 attcgtagcg gaagaaatag cgcgctgcgt gccgcttgcc gtcgtagaaa tcgccatcgc
    14641 ggccgtgcgc cgccaccacc tgctgcagcc acatccacgc gaccacgatg tgtccgaacg
    14701 cctcgagata gatgacgctg ttggccagag cggcctcaaa gtcacccgag ccgaacatcg
    14761 cggtggtgac ctcgaccagg cgctgccacg ccgcgtcgag ctgggccgcc tgttcggccg
    14821 gctcaccgtt gagcgcggcg gcctcggcca cgctccggcc aatcgcgtcg cctagagccg
    14881 cgagccccgc accgccgcgc atggttacct ttcggcccaa caggtcgagg ctctggatgc
    14941 cgtgcgtgcc ctcgtggatg gggttgagcc ggtt
//
LOCUS       DASYLX010001100         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1507793,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001100 DASYLX010000000
VERSION     DASYLX010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507793"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGP27_25760"
     CDS             <1..744
                     /locus_tag="VGP27_25760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854742.1"
                     /translation="ERRVAQDHARTMILSVVTSGHSTFHQDNVVSDVGRGDVVAYCST
                     KPYSATFDNVAKHTFMIDYAALDLPDGVVESHVGQRINRGHVLGGIVARYLVDLGTHA
                     IYLPDADRQALEQPTLDLLRALFTTTAGDDTRAREPLHATLGVRMIEYLKMHLRDPDL
                     NIGRLAREHGISERYAYLILSRSGITLADWLRTQRLAGAARDLANATHPHDTIAAIAY
                     SWGFPDQANFTRAFRRVYGISPREYRRTQ"
     gene            1544..3577
                     /locus_tag="VGP27_25765"
     CDS             1544..3577
                     /locus_tag="VGP27_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV7854743.1"
                     /translation="MSYTAADITELDDVQHTRLRPAVNLGLDVLNTALRELVDNAIEE
                     VADPSHGGSTVTITLHADGSVSVADDGRGLPIDSDPVNGKNGIVKTLGTARAGGKFST
                     HADAASTGAGLNGIGAAAAVFISARTDVTVRRAGKTYLQSFGRGYPGVFDGKDFDPHA
                     PFTRADTQKLRGSGNRKPDAHGTTVRILFDAAVVPDSSVDINEVLLRAHAASRMSPGV
                     HLIVVDEGWPGVAVRAELIEPFSGPWGTDTLLDLMCTAAGTPVPGVRAVVEGRGEYTT
                     GRGPTPFRWSLTAGPAEPAAVAAFCNTVRTPGGGSHLTAAVKGLSEALADRASRIRDL
                     GLAKGEDGPEAQDFAAVTALAVDTRAPDVAWDSQAKTAVSSRSLNVAMAPDVARGVTI
                     WAANPGNGDTVTLWTKLALEAARARRSAEGAKARSRAASKAKGLGTNLSLPPKLLPSR
                     ETGRGSGAELFLCEGDSALGTIKAARDATFQAAFPLKGKPPNVYGFTLSKARVKDEFD
                     SIERILGCGVRDNCDPESCRYDRILFASDADPDGGNINSSLISMFLDFYRPLVEAGLV
                     YVTLPPLFVVKDGQQRIYCQDESERDAAVAQLRANSKRKVEVQRNKGLGEMDADDFWN
                     TVLDPQRRTVIRVHLDEGEAKLHHTLFGGPPEGRRTWMADIASRVDTSALDLD"
     gene            3587..5716
                     /locus_tag="VGP27_25770"
     CDS             3587..5716
                     /locus_tag="VGP27_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV7854744.1"
                     /translation="MTAIIEQNPDLVLDQSADDYWNRYQLTFALYSVSDRAIPSAFDG
                     LKPGQRRLLYQMHESRLLPGNKPQKSSKVCSAVTGNLHPHGGASMYGAAALMAAEFQR
                     VKVIDGQGAFPRIQGDIPAADRYTEMRLSAPGAALTAELNDHAVPMVQTFDGEWIEPT
                     VLPAQWPVLLCNGAVGIAEGWATKVPAHNPREVMAACRALLKTPSMTDDRLVKLIPGP
                     DWGCGATVVGATGLREYITTGRGAFTVRGTVSVDGKNVIITELPPGVASNTVQERIRA
                     LVESGEMSGVADMSDLTDRRNGLRIVVTAKRGHSAETIREQLLALTPLESTFAASLVA
                     LDEDRVPRWWSVRELISAFLHLRDSVVLRRSEYRLEKVTARRHLVAGLMTIHLDIDAA
                     VAVIRGSDTVDEARQGLQERFAIDTGQADYVLALQLRRLTKLDVIELQAEAEKLDAEF
                     LELTELVSNPDARRKVIDNELVETAKLFKGTEFDRRTVLDFDATPTASSADEDGARER
                     KSNAAWRLDDRGVFSDRHGDLLTSGLGWAVWADGRIKFTTGGGLPFKIRDIPVAPDIT
                     GLLRSGVLADGYHLALVTRRGKILRIDPAAVNPQGAAGNGVAGVKLAADGDEVIAALP
                     ISCENGEAILSISEKGWKVTEVADIPVKGRGGAGVGFHPFVNGEAALLAAAVSATGFV
                     RGKRAVRAEKRAKASVKGSGVDVAPAT"
     gene            5823..>6330
                     /locus_tag="VGP27_25775"
     CDS             5823..>6330
                     /locus_tag="VGP27_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HEV7854745.1"
                     /translation="MSHRNARTTFHGRLLIVRRYQAGWAKAHIAKAMGVSRKCVHTWI
                     GRFELEGEIGLMDRSSRPHTTPSRVSNRLENQIVAWRRRHRCGPDEISAKLGVCARTV
                     SRVLRRRGVPYLRDCDPMTGQVIRASKATAVRYERPRPGELVHMDVKKLGRIPDGGGW
                     RAHGRGNTG"
BASE COUNT         1147 a         2091 c         2085 g         1007 t
ORIGIN      
        1 gagcggcgcg tggcgcaaga tcacgcgcgc accatgatcc tcagcgtcgt cacctcgggg
       61 cactccacgt ttcaccaaga caatgtggtc tccgacgtcg ggcgcggcga cgtcgtggcc
      121 tactgctcga ccaagcccta cagcgcgacg ttcgacaacg tcgccaaaca caccttcatg
      181 atcgactacg ccgccctcga tctgcccgac ggcgtcgtgg aatctcatgt gggccaacgc
      241 atcaacaggg gtcacgtcct gggtggcatc gtcgcccgct acctcgtcga cctcggtacc
      301 cacgccatct acctacccga cgccgacagg caagccctcg agcagccgac cctcgatttg
      361 ctgcgcgccc tgttcaccac caccgccggc gacgacacca gggcccgcga accactgcac
      421 gccaccctgg gcgtgcgaat gatcgaatac ctcaagatgc atctacgtga ccccgacctc
      481 aacatcggtc gcctcgcccg cgaacacggc atctccgagc gctacgccta cctgatcctg
      541 tcccgctctg gcatcacgct ggccgactgg ctgcgcaccc agcgcctcgc cggggccgcc
      601 cgcgacctcg ccaacgccac acatccccac gacaccatcg ccgccatcgc ctacagctgg
      661 ggcttccccg accaagccaa cttcacccgc gccttccgcc gcgtctacgg catcagccca
      721 cgcgaatacc gccgaacaca atgacgcacg ctgctgtaag cgtgcttatc gcaatattgc
      781 ggcgtattcg gcatcgcgtt cgcccactac cgccgggaag cagggctggc gacagcgggc
      841 cgaaggctcg gtgattgtgt tgccaacgga ggcgagcgtc gattgcgcgg atgatgcttt
      901 gtcgagcgcg aaatacgggc gagcgtacaa ctttcatggg taatagagac tatccaacaa
      961 atgacttcgt aaatctattg cggctcagca gtattgaccg tactggcctg cgataggtta
     1021 gaccggtcac catgacgaat tgcgctccgg cggcgagccg cgattgctgt cgaggtggtt
     1081 ggactctcga cgggcagtcc ggagcgggtg taggccttat cgccggcggg acagtgcggg
     1141 tgaggcgtac gagagcatcg gcgttgacgg caaccacggt gtgctggcga tcggggtgcg
     1201 ccgcccgaca gcggggcgtg cgtacctcac agtgacgaaa aggttctgct aactacgcgc
     1261 cgacacgaac agtcggcgta gccctcgcac gagcagaatc gggtagttct gaagaaatga
     1321 atctgagact gaagccagat gacatcaagt gagacagtcc acgttacggc aggtcagcgc
     1381 cacgagatat gacacggggt ttgagaagcg acagtcctgg aatccaccga cacggcgggc
     1441 gctgaagaga gcccggtctc acaccgccga ggcagcgcaa agatgtctga ccggccgtct
     1501 accattcctg cggcgcaccc atgactgatg gaaaggacga gcagtgagtt acaccgccgc
     1561 tgacatcacc gagctcgacg atgtccagca cacacgcctg cggccggcgg tgaacctcgg
     1621 tctggacgtg ctcaacaccg cgcttcgcga actggtggat aacgcgatcg aagaggtcgc
     1681 cgatcccagc cacggcgggt ccaccgtcac catcaccttg cacgccgacg gctcggtcag
     1741 cgtcgccgac gacggccgtg gcctgcccat cgactctgac ccggtgaacg ggaagaacgg
     1801 aatcgtcaag acgctgggta ctgcgcgggc gggcggcaag ttctccacac acgccgacgc
     1861 tgccagcacg ggcgccggcc tgaacggaat cggtgccgcc gcggcagttt tcatctcggc
     1921 gcgaactgac gtgacggtac ggcgggctgg gaagacctac ctgcagagtt tcggccgcgg
     1981 ctaccccggg gtgttcgacg ggaaggactt cgacccccac gccccgttca cccgcgccga
     2041 cacacagaag ctacgcggct cgggcaatcg caaaccggat gcacacggca ccacggtgcg
     2101 catcctgttc gacgcggccg tggtgccgga ttccagcgtg gacatcaacg aagtactgct
     2161 gcgggcgcac gccgcctccc ggatgtcccc cggtgtgcac ctgatcgtcg tcgacgaagg
     2221 ctggcctggc gtagccgtcc gggccgaatt gatcgaaccg ttcagcggcc cgtggggcac
     2281 cgataccctc ctggatctca tgtgcaccgc cgcgggcact ccggtgccag gcgtgcgggc
     2341 agtggtggag ggccgcggcg agtacaccac cggccgcggc ccgaccccgt ttcgctggtc
     2401 cttgacggcc ggccccgcgg aaccggccgc cgtcgccgcg ttctgcaata ccgtgcgcac
     2461 ccccggtggc ggatctcatt tgaccgcagc ggtgaaggga ttgtcggaag cactggccga
     2521 tcgggcttcc cgcatccgcg acctgggcct ggccaaaggc gaagatggcc cggaggcaca
     2581 ggatttcgcc gccgtcaccg cgctggccgt cgacacccgc gcacccgatg tggcatggga
     2641 ctcccaagcc aagaccgcgg tgtcgtcgcg ctccctgaac gtggcgatgg cccccgacgt
     2701 ggcgcggggc gtcaccatct gggccgccaa ccccggcaac ggcgacaccg tgacgctgtg
     2761 gaccaagctg gcgctcgagg ccgccagggc gcgacgcagc gccgagggtg ccaaggcccg
     2821 gtcccgggcg gcgtcgaaag ccaagggcct ggggacgaac ctgtcgctgc cgcctaagct
     2881 gctgcccagc cgggagaccg ggcggggctc gggcgccgag ttgttcctgt gcgagggcga
     2941 ctcagcgctg ggcacgatca aagccgcacg cgacgccacc ttccaggcgg cctttccgct
     3001 gaaaggtaaa ccgcccaatg tctatggatt caccctgagc aaggcgcggg tcaaggacga
     3061 attcgactcg atcgaacgca tcctgggctg cggggtgcgt gacaactgcg acccggagtc
     3121 gtgccggtat gaccggatct tgttcgcctc tgacgccgac cccgacggcg gcaacatcaa
     3181 ctcgagcctc atctcgatgt tcctggactt ctaccggcca ctggtcgagg ccgggctggt
     3241 ctacgtgacg ctgccaccgc tgttcgtggt caaagacggc cagcagcgaa tctactgcca
     3301 ggacgagtcc gagcgcgatg cggctgtggc ccaactgcgc gccaactcga aacgcaaagt
     3361 ggaggttcag cggaacaagg gtctcggcga gatggacgcc gacgacttct ggaacaccgt
     3421 gctggatccg cagcgacgca ccgtcattcg ggtacacctc gatgagggtg aagcgaagct
     3481 gcaccacact ctgttcggtg ggccgccaga gggccggcgc acgtggatgg ccgatatcgc
     3541 ctcccgcgtc gacacctctg ctctcgacct cgattaggag aacgacgtga ccgccatcat
     3601 cgagcagaac cccgacctgg tgctcgacca gagtgccgat gactactgga accgctacca
     3661 gctgaccttc gcgctctaca gcgtcagcga ccgtgccatc ccgtccgcgt tcgacgggct
     3721 caagcccggc cagcggcgcc tgctgtacca gatgcacgag tcgagactgc tgcccggaaa
     3781 caagccgcag aagtcctcga aggtctgctc ggcggtcacc ggcaatctgc acccgcacgg
     3841 cggcgcgtcg atgtacgggg ctgcggcgct gatggccgcc gagttccagc gcgtgaaagt
     3901 cattgacgga cagggtgctt ttccgcgtat ccagggagac attcccgccg cggaccgcta
     3961 taccgagatg cggctgtcgg cacccggtgc cgcactgact gccgagctca acgaccacgc
     4021 cgtgccgatg gtgcagacgt tcgacggtga atggatcgag ccgacggtgc tgccggccca
     4081 gtggccggtg ctgctctgca acggcgccgt cggcatcgcc gaaggctggg ccaccaaggt
     4141 gcccgcacac aatccgcgtg aggtcatggc cgcctgccgg gcgctgctga agaccccgag
     4201 catgaccgac gacaggttgg tgaaactcat ccccggcccc gactggggat gcggggccac
     4261 cgtggtcggt gcgaccggac tgcgggagta catcacgacc ggccgcggtg cgttcaccgt
     4321 gcgcggcacg gtgtcggtgg acggaaagaa cgtcatcatc accgagctgc cgcccggcgt
     4381 cgcgagtaac actgtgcaag aacggatccg ggctctggtc gagtcgggcg aaatgtccgg
     4441 cgtggcggac atgtcggacc tgaccgaccg ccgcaacggg ctgcgcatcg tcgtcaccgc
     4501 caaacgcggc cacagtgccg aaacgatccg cgagcagctg ctcgcgctga cgccgctgga
     4561 gtcgacgttc gccgccagtt tggtcgcgct cgatgaggac cgcgttccgc gctggtggtc
     4621 ggtgcgggaa ctcatctccg cattcctgca cctgcgcgat tcggtggtgc tgcgccgcag
     4681 cgagtatcgg ctggaaaagg tcaccgcacg gcgccacctg gtggccggcc tgatgaccat
     4741 ccacctggac atcgacgccg cggtggccgt gatccgcgga tccgacaccg tcgacgaggc
     4801 ccgccagggg ctacaggagc ggttcgcgat cgataccgga caagccgatt acgtgctggc
     4861 gctgcagctt cgccggttga cgaagctcga cgtgatcgag ctgcaggctg aagcggagaa
     4921 gctggacgcc gagttcctcg agctgaccga gttggtgtcg aatcctgatg cgcgccggaa
     4981 ggtgatcgac aacgagcttg tggagaccgc gaaactgttc aaaggcaccg aattcgaccg
     5041 tcgcaccgtg ctggactttg atgccactcc gaccgcatcg agtgccgacg aggatggcgc
     5101 gcgcgagcgt aagtccaacg ccgcgtggcg acttgatgac cgcggcgtgt tctccgaccg
     5161 ccacggcgat ctgctgacct ctggtctggg gtgggcggtg tgggccgacg ggcgaatcaa
     5221 attcaccacc ggtggcggac tgcctttcaa gatccgcgat atcccggtag cgccggacat
     5281 caccgggctg ctgcggtcag gcgtgctggc cgacggctac cacctggcgt tggtgacgcg
     5341 gcgcgggaag atcttgcgca tcgaccccgc tgcggtgaac ccgcagggcg cggcaggcaa
     5401 cggcgtggcc ggggtgaagc tggcggccga cggcgacgag gtcatcgcag cgctgcccat
     5461 cagctgcgag aacggtgagg ccatcctgtc gatctccgaa aagggttgga aggtcaccga
     5521 agtcgccgac atcccggtga agggtcgtgg cggcgccggc gtcggcttcc atccgttcgt
     5581 caacggcgag gctgcgctgc tagcggcggc ggtctcggcg accgggttcg tgcgcggcaa
     5641 gagggcggtg cgggccgaga aacgcgccaa ggcttcggtc aagggttccg gcgtcgacgt
     5701 agcacctgcc acctagatgt accgagtcat gacgttagta gcaggcgtgt cggcttgatg
     5761 tgaagagaac ccccgggtgg ggtgtggctt gtcgaaggcc catcacctga ccggaggttc
     5821 tcgtgtccca ccgtaatgcc cgtacgacat ttcatggccg gctgttgata gtgcgccggt
     5881 atcaggccgg ttgggcgaag gctcatatcg cgaaggcgat gggagtgtcg cgcaagtgtg
     5941 tgcacacctg gatcgggcgg tttgaattag agggcgagat cggcctgatg gaccggtctt
     6001 cgcgcccgca cacgacgccc tcccgggtgt cgaaccgcct ggaaaatcag atcgtggcct
     6061 ggcggcgccg tcatcgctgc ggtccagacg agatcagcgc caagctagga gtgtgtgcgc
     6121 gcacggtctc gcgcgtgctg cgccgacgcg gtgtgcccta cctgcgtgac tgcgacccga
     6181 tgacaggtca ggtgatccgc gcctctaagg ccaccgccgt tcgttatgaa cgaccccggc
     6241 caggcgaact ggtgcacatg gacgtcaaga agctgggccg catacccgac ggcggcggtt
     6301 ggcgtgcaca cggccgcggg aacaccggct
//
LOCUS       DASYLX010001101         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2934968,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001101 DASYLX010000000
VERSION     DASYLX010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2934968"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /gene="miaA"
                     /locus_tag="VGP27_25780"
     CDS             complement(<1..515)
                     /gene="miaA"
                     /locus_tag="VGP27_25780"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV7854746.1"
                     /translation="MRPLAIIGPTGTGKSALALAVAERLGGEIVNADAMQLYRGMDIG
                     TAKLPFAERRGIPHHQLDVLDVTETATVARYQPDAAADVERISAAGTPPIIVGGSMMY
                     IQSLLDDWSFPATQPDVRARWEQRLADIGAAALHRELGRVDSAAASSILATDGRRIVR
                     ALEVIELTGKP"
     gene            complement(512..1198)
                     /locus_tag="VGP27_25785"
     CDS             complement(512..1198)
                     /locus_tag="VGP27_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854747.1"
                     /translation="MLSAIAIVPSAPVMVPELAASAAGELTDLREAVFTAASSLPARW
                     IAVGVGPADALIGPERVGTFAGYGVDLRVSLSPDAGDAPGELPLCALITGWVRAQVDA
                     GARAEVRVFCDDHGFEAARDRGRRLRAEVDDAAEPVGVLVVADGAHTLTPPAPGGYDP
                     EMIPVQASLDDALAGGDTEALARLPRAVVGRVGYQVLAGLAGEGPRSARQLYRGAPYG
                     VGYFAGAWHP"
     gene            complement(1222..2094)
                     /locus_tag="VGP27_25790"
     CDS             complement(1222..2094)
                     /locus_tag="VGP27_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7854748.1"
                     /translation="MAKADIAALLALGAAFFIAIGDVIHQRSAHEVTDEPVSHLELFT
                     RLLRNRQWWLGSLVAAAGFALQAAALGLGSVLLVQALLVTSLLFALPINAWASRRRVT
                     RWEWLWAALLAAAVAVIVTVGNPSAGHSRASFEAWTAVVVVLGPALVLCVLGASIVKG
                     PASAILLGVVSGALWGLFAVLTKGVVDRLDGGLWALVRTPELYVWVLVAIAGTAYQQA
                     SFRAGSLTASLPTMTVTEPLVGSVLGVVVLGEALRPGEAGWFILIVAVAVMVVATAAL
                     ARGEAATASARQPA"
     gene            2333..3664
                     /locus_tag="VGP27_25795"
     CDS             2333..3664
                     /locus_tag="VGP27_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEV7854749.1"
                     /translation="MGTTPESMTTTPKPAPRPGPPRPGPRPGPSPSALHGAPVAPAVD
                     PHRYGRVDPDGTVWLISSAGERVIGSWQAGDTEAAFAHFGRRFDDLATEIGLMESRLA
                     SGTGDARKIKSAAASLAETLPTASVLGDVDALAARLTAIVEHADSAAAAEKAKREEHR
                     AEQTARKEALAAEAEDLATTSTQWKAAGDRLREILDEWRTISGVDRKTDDALWKRYSA
                     AREGFNRRRGAHFAELDRERAGARQAKEALCERAEELAGSTDWGPTSAAFRDLLTEWK
                     AAGRASKDVDDALWQRFKAAQDTFFAARNAISAERDAEFRANADAKEALLAEAGRLDT
                     SDLDAARSALRTIGDKWDKIGKVPRERGADLERRLRAIEKKVRDAPSHRIDPEAQARA
                     DQFRERAEQYERQAEKAAAAGRSKDAEDARASAEQWRQWAEAAAEALGRKG"
     gene            complement(3661..4260)
                     /locus_tag="VGP27_25800"
     CDS             complement(3661..4260)
                     /locus_tag="VGP27_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854750.1"
                     /translation="MTAGDHKGGDFEDFKSDIEAAERRIAREIDPGVRALVIAVAVFV
                     LLATFILPHTGGAKGWDVLAGNDAAIGVGISLPSRVFIWLALVFGVGFSMLALLTRRW
                     ALAWIALAGSMLASFTGLLAVWSRQTAVAPHPGPGIGLIVAWLTVIVLTYNWARVVWA
                     RTALQLAAEEERRRAAAKWQTRGLLESLDDDTDGDGPKR"
     gene            complement(4257..5786)
                     /gene="miaB"
                     /locus_tag="VGP27_25805"
     CDS             complement(4257..5786)
                     /gene="miaB"
                     /locus_tag="VGP27_25805"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV7854751.1"
                     /translation="MTSTATTQAGRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRAAD
                     GADADVVVFNTCAVRENADNKLYGNLSHLVPRKQADPDMQIAVGGCLAQKDRDAVLRR
                     APWVDVVFGTHNIGSLPTLLERARHNNAAQVEIVEALQEFPSNLPVARESAYAGWVSI
                     SVGCNNSCTFCIVPALRGKELDRRPGDVLAEVQALVEQGVLEVTLLGQNVNAYGVSFA
                     DPEQPRDRGAFAQLLRACGRIEGLERVRFTSPHPAEFTDDVIEAMAETPNVCPALHMP
                     MQSGSDRVLRAMRRSYRSDRFLSILDKVRTAIPHAAITTDLIVGFPGETEQDFQETLD
                     VVAAARFTAAFTFQYSKRPGTPAAELDGQLPKAVVQERYQRLIDTQERISLEENTAQI
                     GRTVELLVAVGEGRKDARTARMSGRARDGRLVHFTPTGADIRPGDVVTTDVTGAAPHH
                     LIADGALLAHRRTRAGDAHEDSRRPAGAARPAASGIGIGLGMPGIGVPVSPPTTSGCA
                     L"
     gene            5891..>6306
                     /locus_tag="VGP27_25810"
     CDS             5891..>6306
                     /locus_tag="VGP27_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7854752.1"
                     /translation="MVSIKGVNKHFGNLHVLKDIDLDVDRAEVIVVLGPSGSGKSTLC
                     RTINRLETIESGTIEIDGEVLPEEGRKLAQLRSDVGMVFQSFNLFAHKTILENVALAP
                     RKVRKASKEDARKKAMELLDRVGVANQADKYPAQLSG"
BASE COUNT         1063 a         2214 c         2190 g          839 t
ORIGIN      
        1 aacggcttgc cggtcagctc gatcacctcg agcgcccgca cgatgcgcct gccgtcggtg
       61 gccaggatcg acgaggccgc cgccgagtcg acccggccca gttcccggtg cagcgcggcg
      121 gcgccgatgt cggccagcct ctgctcccac ctggcccgga cgtcgggttg ggtggccgga
      181 aacgaccagt cgtcgagcaa cgactggatg tacatcatgg atccgccgac gatgatcggc
      241 ggcgttccgg cggccgagat ccgctcgacg tcggcggcgg catccggttg atagcgcgcg
      301 acggtcgcgg tctcggtgac gtccagtacg tcgagctgat ggtgcgggat gccgcggcgc
      361 tcggcgaagg gcagcttcgc ggtgccgatg tccatcccgc ggtagagctg catggcgtcg
      421 gcattcacga tctctccgcc aagccgctcc gccaccgcca gtgccagcgc cgacttgccg
      481 gtgccggtgg ggccgatgat cgcgagcggc ctcatgggtg ccaggcgccg gcgaaatagc
      541 cgacgccgta cggcgctcca cgatagagct gcctcgccga acgcgggcct tcgccggcga
      601 gtccggccag cacctggtag ccgacccggc cgacgacggc ccgcggcagc cgggcgagcg
      661 cctcggtgtc gccgcctgcc agcgcgtcgt ccagcgaggc ctgcaccggg atcatctccg
      721 ggtcgtagcc gcccggcgcg ggcggtgtga gggtgtgggc tccgtcggcc acgacgagca
      781 cgccgacggg ttctgcggcg tcgtcgacct cggcgcgcag ccgcctgccg cggtcgcgcg
      841 ccgcctcgaa accgtggtcg tcgcagaaca cccgcacctc ggcgcgcgct ccggcatcga
      901 cctgtgcgcg cacccagccg gtgatcagcg cgcagagcgg cagttcgccg ggggcgtcgc
      961 cggcatccgg cgacagcgag acgcgcaggt cgacgccgta gcccgcgaat gtgccgaccc
     1021 gttcgggacc gatgagcgcg tcagcgggac cgacaccgac ggcgatccag cgcgcgggca
     1081 gcgacgaggc cgccgtgaac accgcctcgc gcaggtcggt cagttcaccg gcggccgacg
     1141 cggccagctc gggaaccatg accggtgccg acgggacgat cgcgatggcg ctcagcacga
     1201 aaccaaacta acgggccgac ttcatgcggg ctgcctcgcc gacgcggtcg cggcctcacc
     1261 acgcgccagc gcggccgtcg cgaccaccat cacggccacc gcgacgatga ggatgaacca
     1321 gcccgcctcg cccggacgca gcgcttcgcc cagcacgacg acgccgagca ccgacccgac
     1381 gagcggttcg gtgaccgtca tcgtgggcag cgacgccgtc aacgaaccgg cccggaagga
     1441 cgcctgctga tacgccgtgc ccgcgatggc gaccagcacc cacacataca gctcaggtgt
     1501 gcggaccaac gcccacaacc cgccgtcgag ccggtccacc acgcccttgg tcagcaccgc
     1561 gaacaagccc cacagcgcac ccgacaccac cccgagcagg atcgcgctgg cgggcccctt
     1621 gacgatgctg gcgcccagca cgcagagcac cagcgcgggg ccgaggacga ccacaaccgc
     1681 cgtccaggcc tcgaacgagg cgcgcgaatg gcccgcagac gggttgccga cggtcacgat
     1741 caccgccacc gccgccgcga gcagcgccgc ccacagccac tcccaccggg tcaccctgcg
     1801 gcggctcgcc catgcgttga tcggcagcgc gaacagcagt gaggtgacca gcagcgcctg
     1861 cacgagcagc accgaaccca gaccgagcgc cgccgcctgc agcgcgaacc cggccgccgc
     1921 gaccaggctg ccgagccacc actgccggtt gcggagcagc ctggtgaaca gctcgaggtg
     1981 gctgacgggt tcgtcggtga cctcgtgtgc ggagcgctga tggatgacgt cgccgatcgc
     2041 gatgaagaag gcggcgccga gggcgagcag ggcggcgatg tccgccttgg ccatctcctg
     2101 gacctcccgt cgtcgtcggt gacgagccta ggcaaatggc agcatcggcg tacggacgca
     2161 cggcgacctg tttgaatagc gtgcgaagta gacgggcgcc gcgtcgcgcg gcaggtgcgc
     2221 ggaaatgtcc gcgcggaggg cacgaggaac gaagatgacg accagcgaac ccggcgggag
     2281 gaccgacgcc cgtagggcag aagacggtgg cggcaccgcc ccgccggagg tcatggggac
     2341 gaccccggaa tcgatgacga ccacccccaa acccgctccc cgtcccggac caccgcgtcc
     2401 gggcccacgg cccggtccgt caccgtccgc ccttcacggc gcgcccgtcg cccctgcagt
     2461 cgacccgcat aggtacggtc gcgtcgaccc cgacggcacg gtgtggctga tcagcagcgc
     2521 aggcgaacgg gtcatcgggt cctggcaggc aggcgacacc gaagcggcct tcgcccattt
     2581 cggacggcgc ttcgatgacc tcgccaccga gatcgggttg atggaaagcc ggctggcgtc
     2641 gggcaccggc gatgcccgca agatcaagtc ggccgcggca tccctcgccg aaaccctgcc
     2701 caccgcatcg gtgctcgggg acgtcgacgc gttggcggcg cggctgacgg cgatcgtcga
     2761 gcacgccgac tccgcggccg cggccgaaaa ggccaagcgc gaggagcacc gcgccgagca
     2821 gaccgcccgc aaggaggcgc tggccgccga ggccgaggac ctggcgacca cttcgacgca
     2881 gtggaaggcc gccggggacc ggctgcggga gatcctcgat gagtggcgca cgatcagcgg
     2941 ggtcgaccgc aagaccgatg acgcgctgtg gaagcggtat tcggcggcgc gggaaggttt
     3001 caaccgccgc cgcggggcgc atttcgccga actcgaccgc gaacgggcgg gcgcccgcca
     3061 ggcgaaggag gcgttgtgcg aacgcgccga ggaactggcc ggctcgactg actggggacc
     3121 cacctcggcg gcctttcgtg acctgctgac cgagtggaag gcggccggcc gcgccagcaa
     3181 ggacgtcgat gacgcgctgt ggcagcggtt caaggccgcc caggacacct tcttcgcggc
     3241 ccgtaacgcc atatccgccg aacgcgacgc cgagttccgc gccaacgcgg atgcgaaaga
     3301 ggcgctgctc gcggaggccg ggagactgga cacctcggat ctggatgccg cgcgttccgc
     3361 cctgcgcacc atcggcgaca aatgggacaa gatcggcaag gtgccgcgtg agcggggcgc
     3421 cgacctggag cggcggctgc gggcgatcga gaagaaggtg cgcgacgcgc cgagccaccg
     3481 catcgatccc gaggcgcagg cgcgcgccga tcaattccgg gaacgcgccg aacagtacga
     3541 acggcaggca gagaaggcgg ccgcggcagg ccgttccaag gacgccgagg acgcgcgggc
     3601 gagcgccgag caatggcggc agtgggccga agccgcggcc gaagctctcg gcaggaaagg
     3661 ctaacgcttc ggtccgtcgc cgtcggtgtc gtcgtcgagg ctctccagca ggcccctggt
     3721 ctgccacttg gcggccgccc tgcgacgctc ctcctcggct gcgagctgca gcgcggtgcg
     3781 tgcccacacc acccgggccc agttgtaggt cagcacgatc acggtgagcc aggcgacgat
     3841 caggccgatg ccgggcccag ggtgcggtgc gacagcggtc tgccgcgacc acaccgccag
     3901 cagcccggtg aaggacgcca gcatggatcc ggccagcgcg atccacgcca acgcccatcg
     3961 tctggtcagt agggccagca tcgagaagcc gaccccgaac accagcgcca gccagatgaa
     4021 cacgcgcgac ggcagggaga tcccgacccc gatcgcggca tcgttgcctg ccagcacatc
     4081 ccagcccttg gcgccgccgg tgtgcggcag gatgaacgtc gccagcagta cgaaaaccgc
     4141 aacggcgatg accaacgccc tcacgccggg atcgatttcg cgggcgatgc ggcgttcggc
     4201 ggcctcgata tcgctcttga agtcctcgaa gtctccgccc ttgtgatcgc ccgcggtcac
     4261 agagcacacc ccgaggtggt cggcgggctg accggcaccc cgatgccagg catgccgagg
     4321 ccgataccga tgcccgatgc cgccggccga gcggctcccg cgggccgacg ggagtcctcg
     4381 tgcgcgtcac ctgcgcgggt acgccgatgc gccagcagcg ccccgtcggc gatcagatgg
     4441 tgcggcgcgg cgccggtgac gtcggtggtg acgacgtccc cgggccggat gtcggcgccc
     4501 gtcggggtga agtgcaccaa cctgccgtcc ctcgcgcgac cggacatcct cgcggtgcgg
     4561 gcgtccttgc gcccttcgcc gacggctacc aacagctcga cggtgcggcc gatctgggcg
     4621 gtgttctcct ccagcgagat tcgttcctgg gtatcgatga gtcgttgata acgttcttgc
     4681 acaacggctt tcggtagctg tccgtcgagc tcggccgccg gcgtgccggg tcgcttcgaa
     4741 tactggaacg tgaacgctgc ggtgaaccgg gccgcggcca ccacgtcgag cgtttcctga
     4801 aagtcctgct cggtctctcc agggaatccg acgatcaggt cggtggtgat cgcggcgtgc
     4861 gggatggcgg tgcgcacctt gtcgaggatg gacaggaagc gatcggagcg gtaggaccgg
     4921 cgcatggccc gcagcacccg gtccgagccg gactgcatcg gcatgtgaag ggccgggcac
     4981 acattcggcg tctccgccat cgcctcgatg acgtcgtcgg tgaactcggc ggggtgaggc
     5041 gaggtgaacc ggacccgttc cagcccctcg atccggccgc aggcgcgcag aagttgggcg
     5101 aacgccccgc gatcgcgtgg ctgctcgggg tcggcgaacg agacgccgta ggcgttgacg
     5161 ttctggccga gcagcgtcac ctcgaggaca ccctgctcca ccagtgcctg cacctcggcc
     5221 agcacatcgc ccggcctgcg gtcgagctcc ttgccgcgca acgcgggcac gatgcagaac
     5281 gtgcagctgt tgttgcagcc aaccgagatc gaaacccaac cggcgtaagc ggattcccgc
     5341 gccaccggca aattcgacgg gaactcctgc agtgcctcga cgatttcgac ctgcgcggcg
     5401 ttgttgtgcc gagcccgctc gagcagcgtc ggcagcgacc cgatgttgtg tgtgccgaag
     5461 acgacatcca cccacggggc ccggcgcagc acggcgtcgc ggtccttttg cgccagacac
     5521 ccgccgacgg cgatctgcat atccgggtcg gcctgtttgc gcggcaccag atggctgagg
     5581 ttgccgtaga gcttgttgtc ggcattctcc cgcacggcac acgtgttgaa caccacaacg
     5641 tcggcgtccg cgccgtcggc cgcacgccgg tagcccgccg cctcgagcag gcccgccagc
     5701 cgctcggagt cgtgcacgtt catctggcag ccgtaggtgc gcacctgata ggtgcgccca
     5761 gcctgcgtgg ttgccgtcga tgtcaccagg cccatgttac gggcggcaat acggcgttaa
     5821 ttgagcggat tcacgagttg aagacaacct gcgcgttacc tgggtaaggt ctgcatccat
     5881 gggcacgccg atggtctcga tcaagggcgt caacaagcat ttcggcaatc tccatgtgct
     5941 caaggacatc gacctcgacg tcgaccgtgc cgaggtgatc gtcgtactgg ggccgtccgg
     6001 ctccggcaaa tcaacgttgt gccgcaccat caatcggctc gagaccatcg aatcgggcac
     6061 gatcgagatc gacggtgagg tgttgccgga ggaaggacgc aagctcgcgc agctgcggtc
     6121 cgacgtcggc atggtgtttc agtccttcaa tctgttcgcg cacaagacga ttctggaaaa
     6181 cgtcgcactg gcaccccgca aggtgcgcaa agcgtccaag gaggacgcgc gcaagaaggc
     6241 gatggagctg ctggaccgcg tcggcgtggc caaccaggcc gacaagtacc ccgcacagct
     6301 atcggg
//
LOCUS       DASYLX010001102         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_520093,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001102 DASYLX010000000
VERSION     DASYLX010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520093"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VGP27_25815"
     CDS             complement(<1..908)
                     /locus_tag="VGP27_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEV7854753.1"
                     /translation="MLDAPERVETTADEAEHPTRKVTWWRSLHATSTRRALLLTALGG
                     LLIAGVLTALPRGEGPSTPGAITLGLRGAETFSKAKSGDCLNWPERNPDGAQVVDCKS
                     EHRFEVAESVDMQTFPGSEYGPDAEPPSPSRIQQISQEQCQVAVRRYLGGRFDPNSRF
                     TVSMMWSGDKAWRQSGERRMLCGLQLPGANNSQLAFQGKIAEVDQSKVWPAGTCLGID
                     PSTNQPTDIPVDCAGPHAMEVTGAVNVAEKFPARLPPEPDQDAFIKDACTRMTDAYLA
                     PIQLRSTTLTLIYSTLSLPSWSAGSH"
     gene            999..>1913
                     /locus_tag="VGP27_25820"
     CDS             999..>1913
                     /locus_tag="VGP27_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEV7854754.1"
                     /translation="MIDLRLLRENPDAVRASQRARGEDPGLVDVLLEADTARRAAVSA
                     ADNLRAEQKAASKRVGAASKDERPALLQEAKALADKVKAAESAQADADRAFTAAHMAI
                     SNVVMDGVPSGGEDDFVVLDTVGEPPAIPDPKDHVALGESLGLFDLERGAKVSGARFY
                     FLTGYGALLQLGLMQLAVRLATDNGFTLVIPPVLVRPEIMEGTGFLGAHSEEVYRLEA
                     DDLYLVGTSEVPLAGYHSDEILDLSAGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQF
                     DKVEGFVYCKPEEAEAEHERLLGWERQML"
BASE COUNT          288 a          624 c          679 g          322 t
ORIGIN      
        1 tggtggctgc ccgccgacca gctgggcagc gaaagggtgc tgtagatcag cgtgagtgtg
       61 gtgctgcgca actggatggg cgccagatag gcatcggtca tccgggtgca ggcgtccttg
      121 atgaacgcat cctggtcggg ctcgggtggc agccgggcgg ggaacttctc ggcgacgttg
      181 acggcgccgg tcacctccat ggcatgcggg cctgcgcagt ccaccgggat gtcggtcggc
      241 tggttggtgg acgggtcgat gcccaggcac gtgcccgcgg gccagacctt ggactggtcc
      301 acctcggcga tcttgccctg aaacgcgagc tgagagttgt tggcgccggg cagttgcagg
      361 ccgcacagca tgcgccgctc gccggactgc cgccacgcct tgtcgcccga ccacatcatg
      421 ctgacggtga agcggctgtt cgggtcgaat cgcccgccaa gatagcggcg caccgccacc
      481 tggcactgct cctggctgat ctgctggatg cgcgacggtg acggcggctc ggcgtcggga
      541 ccgtactcgc tgccagggaa ggtctgcatg tcgaccgact ccgcgacctc gaaccggtgc
      601 tcgctcttgc agtcgacgac ctgggcgcca tccgggttgc gttctggcca gttcaggcag
      661 tcgccgctct tggccttgct gaacgtctcc gccccgcgca ggcccagcgt tatcgcgccg
      721 ggggtactcg ggccctcgcc gcgcggcagc gcggtgagca cgccggcgat caggagtccg
      781 ccgagagcgg tcagcagcag cgcccgccgg gtggacgtgg cgtgcaaact gcgccaccac
      841 gtcaccttgc gggtggggtg ttctgcctcg tcggcggtcg tctcgacccg ctcgggtgca
      901 tccaacatct gctctattgt gacaggcgcg tcgggggctg tgacaagtga ggcagatgta
      961 acgttatgag gttgtgcccc cgacggtagg gttgcgccgt gatcgacctc aggctcctgc
     1021 gcgaaaaccc ggatgcggtg cgggcgtcac agcgcgcccg cggtgaggat ccgggcctcg
     1081 tcgacgtgct gttggaggcc gacacggccc gtcgtgccgc cgtgtcggcg gccgacaacc
     1141 tgcgcgccga acagaaagcc gcgagcaaaa gggtcggcgc ggcgtccaag gacgagcgtc
     1201 ctgcgctgct gcaggaggcc aaggcgctcg cggacaaggt gaaggcggcc gagtccgcgc
     1261 aggcggatgc ggacagggcg ttcaccgccg cgcatatggc gatatccaat gtcgtcatgg
     1321 acggggttcc ctccggcggc gaagacgact tcgtggttct cgataccgtc ggcgaacctc
     1381 cggccatccc cgatccgaaa gaccatgtgg cccttggtga atcgctcggg ctgttcgacc
     1441 tggaacgcgg cgcgaaggtt tccggcgcac ggttctactt cctcaccggc tacggcgcgc
     1501 tgctgcaact cggcctgatg cagctcgcgg tccggctcgc tacagacaac ggcttcacgc
     1561 tcgtcatccc gccggtcctg gttcggcccg agatcatgga gggcaccggg ttcctcggcg
     1621 cgcacagtga agaggtgtac cggctcgagg ccgacgacct ctacctcgtc ggcacgtcgg
     1681 aggtgccgct ggccggctac cactccgacg agatcctcga tctgtccgca ggcccgctcc
     1741 gctacgcggg ctggtcgtcg tgcttcaggc gcgaggcggg cagccacggc aaggacaccc
     1801 gcggaatcat tcgcgtgcac cagttcgaca aggtcgaggg cttcgtctac tgcaagccgg
     1861 aagaggcgga ggccgaacac gagcgcctgc tcggctggga gcgtcagatg ctg
//
LOCUS       DASYLX010001103         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3262921,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001103 DASYLX010000000
VERSION     DASYLX010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3262921"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1775)
                     /locus_tag="VGP27_25825"
     CDS             complement(309..1775)
                     /locus_tag="VGP27_25825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7854755.1"
                     /translation="MAIPAAPDTLDPALTQSRYAAAIMPTYCEKLFDITPDQNIVPML
                     ATALPTVSPDGKTYTIKLRSGVQFNDGTPFDAQAVKTSLDRSRTDKASAQVANLSAIQ
                     DVSVTDPTTVKLTLKSPSAPLTSVLADRAGMILSPTALTKEGAQFGTAPVCVGPFSFD
                     SRPSSDRIVFKKSDYYYDKSQVKLNQVTFQVVTQPNIRATNLKSGDVGLAMDIAPADM
                     AGIEGNPSTALLTQNSLGYRGFTINVANTHGVGVPPYTHPSTPLAQSQELRQAFVLSL
                     DRDAINKAVYNGEEYPSCSPISSANPLYSDPGCPGQDLTKAKQLVAQSGIPTPIPVTV
                     IIGASDDLQTKLATVIQSMAKEAGFAVTIKPEENVTAGNDAQKGNFDIYMNSWSGRLD
                     PDQNIEVFWSPSSTINYSNANYPDLNALLTQATVTSDTNERKALYRQIVQLQNTYLDD
                     VVLFHDRLVLGASKSVQGVVFLPNDVLELKTASITSGS"
BASE COUNT          352 a          650 c          730 g          422 t
ORIGIN      
        1 gccgtagcgc cgacgccccg tcgaaacagc tgaccgcgag accttgtgcc gcccggaaag
       61 tcatcgccgc agctcggcag tacgcaggag gagcgcgtga tcatcggtgg gtgatcgtgt
      121 cgctagtgga ccgcgtgggc atatcggggt gtcctccgga ctaccgggga tcagtgatca
      181 tccagtgacc cgagaccgcc gagccggggt acatcatgtc ggtgctccgg gatcaagctc
      241 agtcagcagc gcctggccaa tcttgcggag tacgtaccgt ctcacgacgc gacacccact
      301 gatccggctc agctgccgct ggtgatgctg gcggtcttga gctcgaggac gtcgttgggc
      361 aggaacacca caccctgcac gctcttgctc gccccgagca ccagccggtc gtggaagagc
      421 accacgtcgt cgaggtaggt gttctgcagc tggacgatct ggcggtacaa cgccttacgc
      481 tcgttggtgt cggaggtcac cgtggcctgg gtgagcagcg cgttgaggtc cgggtagttc
      541 gcgttgctgt agttgatcgt gctgctgggt gaccagaaca cctcgatgtt ctggtccgga
      601 tccagccggc cggaccagga gttcatgtag atgtcgaagt tgcccttctg ggcgtcgttg
      661 ccggcggtga cgttctcctc cggcttgatg gtcacggcga aacccgcctc cttcgccatg
      721 gactggatca cggtggccag cttggtctgc aggtcgtcac tggcaccgat gatgaccgtg
      781 acgggaatcg gcgtcggaat gccgctctgc gccaccagtt gcttggcctt ggtcaggtcc
      841 tgtccggggc agccggggtc ggagtacagc gggttggcgc tggagatcgg gctgcacgac
      901 gggtactcct cgccgttgta gacggccttg ttgatcgcgt cccgatcgag cgagagcacg
      961 aacgcctgcc gcagttcctg gctctgcgcg aggggcgtgc tcgggtgcgt gtagggcggc
     1021 acgcccaccc cgtgggtgtt ggccacgttg atggtgaacc cccggtagcc caacgagttc
     1081 tgcgtcagca gggcggtgct cggatttcct tcgataccgg ccatgtccgc gggagcgatg
     1141 tccatcgcga ggccgacgtc gcccgacttg aggttcgtcg cccggatgtt gggctgggtg
     1201 accacctgga aggtcacctg gttcagcttc acctggctct tgtcgtagta gtagtccgac
     1261 ttcttgaaca cgatccgatc gctggagggc cgcgagtcga acgagaacgg gcccacacac
     1321 accggtgcgg tgccgaactg cgcgccttcc ttggtcagcg ccgtcggcga gaggatcatt
     1381 ccggcgcgat cggccagcac cgacgtcagt ggcgcggacg gtgatttcag tgtcagcttg
     1441 acggtggtgg gatccgtcac cgagacgtcc tggatcgccg agaggttcgc gacctgagcc
     1501 gaagccttgt cggtccgcga gcgatcgagg ctggtcttga ccgcctgcgc gtcgaacggt
     1561 gtcccgtcgt tgaactggac gccgcttctc agcttgatgg tgtaggtctt gccgtccggg
     1621 ctgaccgtcg gcagcgccgt ggcgagcatc ggcacgatgt tctgatccgg tgtgatatcg
     1681 aacagctttt cgcagtacgt cggcatgatg gcggcggcat accgcgactg ggtcagcgcc
     1741 ggatcgagcg tgtcgggagc cgcgggaatc gccaccgtaa gattccccgc ggtgccggac
     1801 gccccggtac tgccaccacc acctgccgaa catccggcgg ccagtagtgc cgcggcaaac
     1861 acgaggggag cgagccaacg gcgaacgagc atcgcatatt tcctgtccgt aatggtctgc
     1921 gacgaccttg aaattcgctg gacagaggtc agttcacggc gagatggaaa agtggtgacg
     1981 cgcatgaaga acctcgacgg gcgtcgccta cgactcactc gagagtgagc gaaccactgc
     2041 tgcccctttc ccgttgcctt ttccagggat ggcaaccgac gaaacctgct tcgcggcacg
     2101 ggtggcgtcg cgagcgggcg tgatcactct tgttctcggc ggcatgttag tcaa
//
LOCUS       DASYLX010001104         7534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2935397,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001104 DASYLX010000000
VERSION     DASYLX010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2935397"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGP27_25830"
     CDS             complement(<1..120)
                     /locus_tag="VGP27_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854756.1"
                     /translation="MDSTMQDVPLTITAIMRHACDLHGDRTVTTATGDGYRHTT"
     gene            complement(190..1350)
                     /locus_tag="VGP27_25835"
     CDS             complement(190..1350)
                     /locus_tag="VGP27_25835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854757.1"
                     /translation="MGNHDGVVQTSVASQPATATRVRLSHHVVTLDDGHRVGVTVGGQ
                     GVPLVFLHGIALSGRAYVRLLSRLAGMGFLVVALDAAGHGFTPNLPRKEADLTHRVDL
                     TLRALDALGVRKAVFLGHSMGGRMIVQLAAAAPERVLAAVLLNAAAGTPFDESISTPV
                     RSPRRAARALMNAAYDAPGDPRRLPAPERKRYMRQMAGALARNVRTPLGLPGAVRAII
                     ASGDSTPLLEAMREHGVPTIIVHGEKDLVVPFASAQDMAERADATLYRVPGAYHSWML
                     ANPRHGADLIRQLLAAELGDVLRDTARAHGIVGVAGGWQDALLTPDSRLHSLEDRPLE
                     AVGVEPLEHIELDRLRSGRRSPPQPADRRFRWFDGWRRRRGQSPSDGTADIA"
     gene            1516..1839
                     /locus_tag="VGP27_25840"
     CDS             1516..1839
                     /locus_tag="VGP27_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854758.1"
                     /translation="MRKNLTLAAGIVGVCAAMTVPAGIASAEEDAQATIKRLQDEGYT
                     VNIDRTGSAPMSECVVTNVRNPKQVTQWVPYIGPGRGGFGGDRALVLDVVSQSISVTL
                     DCNRR"
     gene            complement(1907..3316)
                     /locus_tag="VGP27_25845"
     CDS             complement(1907..3316)
                     /locus_tag="VGP27_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEV7854759.1"
                     /translation="MTVIDRQPPVAEPPDPPRRKDRTHWLYIAVIAAVLVGIAVGILA
                     PDVGKSVGVLGTMFVALIKMMIAPVIFCTIVLGIGSVRKAATVGKVGGLAFVYFLVMS
                     TFALAIGLVVGNLIHPGSGMHLSESASGKGAELAEKAHESGGLTDFVAHIIPDTLLSA
                     LTGSNVLQALFVALLVGFALQAMGAPGEPILRGIEHVQKLVFKILVMILWLAPIGAFG
                     AIANVVGQTGWTAVTQLLTLMLGFYITCVLFVFGVLGTILRVVSGVSIFKLVRYLARE
                     YLLIFSTSSSESALPRLIAKMEHLGVDRSTVGVVVPTGYSFNLDGTAIYLTMASLFIA
                     DALGSPMSVGEQIGLLVFMIVASKGAAGVTGAGLATLAGGLQSHRPDLLDGVGLIVGI
                     DRFMSEARAVTNFSGNAVATVLVGSWTKTVDKDKVNAVLRGDDPFDELTMVDDDHAPR
                     GHEQDRPALTGGAKVPAPA"
     gene            3559..3804
                     /locus_tag="VGP27_25850"
     CDS             3559..3804
                     /locus_tag="VGP27_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854760.1"
                     /translation="MKKNLVRLVAGLLAAAGLAAGAVGFAAAANADDPVKAAPDLFSA
                     HTGPALANAHLVSEPDRATNPAAGIAKIHTSPTHQAG"
     gene            3863..4765
                     /locus_tag="VGP27_25855"
     CDS             3863..4765
                     /locus_tag="VGP27_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854761.1"
                     /translation="MDSGVEDVARAVTTPDGRRLAHLEVGCPNGPLVLHNHGGPSSRL
                     EARLLADVAANNGLRLVCVDRPGMGQSTSQKHRSYSSWAVDLVAVADALGYREFGVTG
                     WSEGGPWALAAAAYIDPARLRHVSSIAPGSYGAFGDNSAAQYLTRADALGGSLALHFE
                     PGFRLMYAALGLTAKRFRSTFVNQIRNGVSDYDRQLLARDDIARGFGDACAECFAHGS
                     DGLVRDAELLYRRWAFDIRTIERPVHLWQGLDDTLVPDPINMAVADAMPGTVWHPVEG
                     AGHFLAIGAGEEIFGIASGEMSTP"
     gene            4762..6318
                     /locus_tag="VGP27_25860"
     CDS             4762..6318
                     /locus_tag="VGP27_25860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7854762.1"
                     /translation="MSRWGPKSLATQAIALQILVIAVVVLAGTGLAVLDARQDADAAA
                     RQQVLAIATALADSPSTAVAIESGRATEVLQPVTEAVRTNTDIAFITIMSPDGTRFTH
                     TDPNQIGGHYLGTIEPAIRGEAFTEVYTGTLGPSVRAIVPVHDGSGRLVGLVAAGITQ
                     QTLAERWRSQWPTIAAVSVGALAISLVGVWAIRRRLLRQTHGLRPGELRVMYEHHDAI
                     LHSVSEGLVVLDRNGVALVNDEARRLLGLPPGPVATSDLPEFLRTYNPGARDEVHVTD
                     ERVLVVNRSQVDNRPQDSEVVTIRDRTELQGALGELSSLQVLTDSLRSQAHEAANKLH
                     TVITMVEMGRADEAVRFATHELQLSQQLVDRLSADVAEPALVALLLGKAAQADERGIE
                     LSVTEDTHLPSNIDDVPLSGQEMVTVLGNLIDNAMDACDRDNPWVEVTVSQDDHTLLL
                     RVADSGSGMDSAAFEKAMQRGYSTKSGVDTGQHGLGLALVAQVVKRHNGTLTADVTYG
                     SVVTVTVSDS"
     gene            6315..7004
                     /locus_tag="VGP27_25865"
     CDS             6315..7004
                     /locus_tag="VGP27_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7854763.1"
                     /translation="MISVLIVEDEPLIAEAHQAYLQRLEGFSVTAVTHTARDAMRAAA
                     TAAANESPIDLVLLDIGLPDASGISLASGLSGLRPAPDIIAITSERDLEMVRAAVGHG
                     ALAYLLKPFTFAAFRDRLERYQRYREALPAGTDAASQAEVDRALAELRVSTDRSAAPK
                     GAAAGTNDEIARAVRDSPDGITADAVAKQVGVSRVTAWRYLERLADEGTVTRHTDYGR
                     AGRPKTRYQWR"
     gene            complement(7040..>7534)
                     /locus_tag="VGP27_25870"
     CDS             complement(7040..>7534)
                     /locus_tag="VGP27_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV7854764.1"
                     /translation="ASVIGLDVPDTYLIGVSALQPHSRGTVRLKSWNPGVPPVVDPNY
                     LGDDRDMHTMLEGIRIARHIGSAHALDDWCAQELAPGPAVDDEESLREYVRATTSSYY
                     HPVGTCAIGQSGRSVVDSELRVHGIDGLRVVDASVMPSLPSNNTLATVYAIAERGAEM
                     IRHG"
BASE COUNT         1294 a         2526 c         2517 g         1197 t
ORIGIN      
        1 ggtggtgtgc cgatacccgt cgcccgttgc ggtcgtgacc gtgcggtcgc cgtgcagatc
       61 gcaggcgtgc cgcatgatgg cggtgatcgt cagcgggacg tcctgcatcg tgctgtccat
      121 cgggtttccc cctccgactt cgggcgccac gcgcggcgcg gccagatgga cgcgatggta
      181 atgcgccgat caggcgatgt cggcggttcc gtccgatggc gattgcccgc gcctgcggcg
      241 ccacccgtcg aaccagcgga aacggcggtc ggccggttgc ggcggcgacc ggcgtccgct
      301 gcgcagccgg tcgagttcga tgtgctcgag cggctcgacg ccgacggcct caagcggcct
      361 gtcctccagc gaatgcaggc gtgagtccgg ggtaagcagg gcgtcctgcc agccaccggc
      421 aacaccaacg atcccgtgcg ccctggcggt gtcgcgcagc acgtcgccga gttcggcggc
      481 cagcaactgg cgaatcaggt cggcgccgtg ccgcgggttg gccagcatcc acgagtggta
      541 ggcgccgggt acccggtaca gcgtggcgtc ggcgcgctct gccatgtcct gcgcactggc
      601 gaacggcact accagatcct tctcgccgtg cacgatgatg gtcggcacgc cgtgctcgcg
      661 catcgcctcc aacagcggcg tggagtcgcc ggaggcgatg atggcccgca cagcgccggg
      721 tagcccgagc ggcgtccgca cattgcgggc cagcgcgccc gccatctgcc tcatgtagcg
      781 cttgcgttcg ggcgcgggca gcctgcgggg atcacctggg gcgtcgtacg cggcgttcat
      841 caaggcccgc gccgccctgc ggggtgaccg gaccggcgtg ctgatggact cgtcgaacgg
      901 cgtgcccgcc gcggcgttca acagcaccgc ggccagcacc cgctcgggcg cggcggccgc
      961 caactggacg atcatccggc cgcccatcga gtggcccaag aagacggcct tgcgcacgcc
     1021 gagtgcatcg agcgcgcgca aggtcaggtc gacacgatgg gtcaggtcgg cctccttgcg
     1081 gggcagattt ggggtgaagc cgtggcccgc cgcgtcgagg gcgacgacga ggaaccccat
     1141 ccccgccaac cggctcagca atcgcacgta ggcccgcccg ctgagggcga tgccgtgcag
     1201 gaagaccagc ggcacgccct ggccgcccac tgtcacgccc accctgtgcc cgtcgtcgag
     1261 cgtcacgacg tggtgggata gccggacccg ggtcgccgtg gccggctggg aagccaccga
     1321 tgtctgaacc acaccatcat ggttgcccat ccgggcgagc gaaggccaac cccgcggatg
     1381 tggggttgat tacggttgcg ccgcccggcg atgaacctca cgacgagcgt cttggcgatc
     1441 cttgggaacg aaatgagtgt cgcaggcgat ctatttgagg gatcattgcc tgagcaaacg
     1501 acgagaggtg cggtaatgcg caagaacttg acgctcgcag ctggcattgt tggagtgtgc
     1561 gcagcgatga ccgtgcccgc aggcatcgcg tcagccgagg aggacgcaca ggcgaccatc
     1621 aagcggctgc aggacgaggg ctacaccgtc aacatcgacc gcaccggcag cgcgccgatg
     1681 agcgaatgcg tggtgaccaa tgtgcgcaat ccgaagcagg taacccagtg ggtgccctac
     1741 atcggccccg gccgcggtgg cttcggcggc gaccgcgcgc ttgtcctgga tgtggtcagt
     1801 cagtccatct cggtgacgct ggactgcaac cgccgctgat taaggcttga tcgcagcgaa
     1861 ggcccggccg gtggcagtac cggccgggcc ttctgcactg cgcgccctac gccggtgcgg
     1921 gcactttcgc gccgcccgtg agcgcgggcc ggtcctgctc gtggccgcgc ggcgcatggt
     1981 cgtcgtccac catcgtcagc tcgtcgaacg gatcgtcgcc gcgcagcacc gcgttgacct
     2041 tgtccttgtc gacggtcttc gtccacgacc cgaccaacac cgtggccacg gcgttgccgg
     2101 agaagttcgt gaccgcccgg gcctccgaca tgaaccggtc gattccgacg atcagtccga
     2161 cgccgtccag caggtcgggt cggtggctct gcaggccgcc cgccaatgtg gccagccccg
     2221 cgccggtgac acccgccgcg cccttggagg cgacgatcat gaacaccagc aggccgatct
     2281 gctcgccaac cgacatcggg ctgcccagcg cgtcagcgat gaacagcgac gccatcgtca
     2341 gatagatcgc ggtgccgtcc aggttgaacg agtaaccggt cggaacgacg actccgacgg
     2401 tgctgcggtc gacacccaga tgctccattt tggcgatcag ccgcggcagc gcggactccg
     2461 acgaggacgt cgagaagatc agcagatatt cacgggcgag gtagcgcacc agcttgaaga
     2521 tcgacacacc cgagaccacg cgcaggatcg tgcccaacac gccgaacacg aacagcacgc
     2581 aggtgatgta gaagccgagc atcaacgtca gcagttgcgt gaccgcggtc cagccggtct
     2641 gtccgacgac gttggcgatc gcgccgaacg cgccgatcgg cgccagccac aggatcatca
     2701 ccagaatttt gaacaccagc ttctgaacat gctcgatgcc gcgcagaatc ggctcccccg
     2761 gggcgcccat cgcctgcaat gcgaagccga ccaacagtgc gacgaaaagt gcttgcagca
     2821 cattgcttcc ggtcaatgcc gacagcagcg tgtccgggat gatgtgcgca acgaagtccg
     2881 tcagaccgcc ggactcgtgc gccttctcgg ccagttcggc ccccttgccc gaggcgctct
     2941 cggacaggtg catgccgctg ccgggatgga tgaggttgcc gacgaccaag ccgatcgcca
     3001 gagcgaacgt cgacatcacg aggaaataca cgaacgccaa gccgccgacc tttccgacgg
     3061 tggcggcctt gcggacagac ccgatgccca acacgatcgt gcaaaagatc accggggcga
     3121 tcatcatttt gatcagcgcg acgaacatcg tgcccaggac gccgacgctt ttgcccacgt
     3181 ccggcgcgag gatgccgacc gcgataccga ccagcacagc ggcgatgacg gcgatgtaga
     3241 gccaatgcgt acggtccttg cggcgcggcg gatccggtgg ctcggctacc gggggctggc
     3301 ggtcgatgac tgtcatgtgt ggacttacct tccaggtcga catcgaatgt gatgtgcaag
     3361 taggttccgc cagcgcgtga gccgggtcac gttttagttc attacgttca gggcgccttg
     3421 cgatgagctc aatgcacaca agccgtggaa tcgcgcactc tgcgggttgt gtgcacaagc
     3481 gagccatgct gacagccggt ccataccttc agcggtggca cttcagccga atgtcgccaa
     3541 caccgaagga gaatcgacat gaagaagaac ctcgtccgcc tcgtcgcggg tctcctggcg
     3601 gccgcaggac tggccgccgg cgctgtgggg ttcgccgctg ccgccaacgc cgacgacccc
     3661 gtgaaagccg ctcccgacct gttttcggcg cacaccggac cggcgctcgc taatgcgcac
     3721 ttggtcagcg agcctgatcg ggccacgaat ccggcagccg ggatcgccaa gatccacacg
     3781 tcgccgacgc atcaagccgg atgactgatc gggaccgggc aatctgaccg ctcctcgggc
     3841 ggcggactgc tttcattgcg tggtggacag cggcgtggaa gacgtggccc gtgcagtgac
     3901 gacccccgac ggtcgcaggc tcgcccatct cgaagtcggc tgtccgaacg gtccgctcgt
     3961 gctgcacaat cacggcgggc cgagcagccg gctcgaggcg cgccttctgg ccgacgtagc
     4021 ggcgaataac gggctgcgcc tcgtgtgcgt ggaccggccg ggcatgggcc agtccacgtc
     4081 tcagaagcac cgttcctact cgagctgggc cgtcgacctc gtcgccgtcg ccgacgcatt
     4141 gggataccgg gaattcggag tgacgggttg gtcggagggc ggcccgtggg cactcgcagc
     4201 ggccgcctat atcgatccgg cgcgcctgcg gcacgtcagc agcatcgcgc cggggagcta
     4261 cggcgcgttc ggagacaact cggcggcgca atacctgacg agggccgacg cgctgggggg
     4321 atcgctggcg ctgcatttcg agccaggttt ccggctcatg tatgccgccc taggtctgac
     4381 cgccaagcgc tttcgcagca ccttcgtcaa ccagatccgc aacggcgtca gcgactatga
     4441 ccggcaattg ctggcgcggg acgacatcgc acggggcttc ggcgatgcct gtgccgagtg
     4501 cttcgcccac ggctccgacg gcctggtccg cgatgctgaa cttctgtatc ggcgttgggc
     4561 tttcgacatc aggacgatcg agcggcccgt tcacctctgg cagggactcg acgacacact
     4621 cgtgcccgat ccgatcaaca tggcggtggc cgacgcgatg cccggcaccg tgtggcatcc
     4681 cgtcgagggc gcgggtcatt tcctggcgat cggtgccgga gaagagatct tcggcatcgc
     4741 gtccggggag atgagcacgc cttgagcagg tggggaccga aatcactggc gacgcaggcg
     4801 atcgcgctgc agatcctggt gattgcagtc gtggtgttgg cgggcactgg tttggcagtg
     4861 ctcgacgccc ggcaggatgc cgacgccgcc gcacgccagc aggtgctcgc catcgccacc
     4921 gcgctggccg actcaccgtc gacggccgtg gccatcgaat ccggacgagc cacagaagtt
     4981 ctacagcccg ttaccgaggc cgtccggacc aacaccgaca tcgccttcat cacgatcatg
     5041 tcgcccgacg gaacgcgctt cacccacacc gatcccaacc agatcggcgg tcactacctc
     5101 ggcaccatcg agccggccat ccgcggcgag gcgttcaccg aggtctacac cggcacactc
     5161 ggcccgtcgg tgcgcgcgat cgtgccggtg cacgacggct ccggcaggct ggtcggtctg
     5221 gtggcggcag gcatcaccca gcagacgctt gccgagcgct ggcgatcgca atggccgacg
     5281 atcgcggcgg tcagcgttgg ggcgctggcg atctcgttgg tcggcgtgtg ggcgatccgg
     5341 cgacggttgc ttcggcagac ccacgggttg cgcccaggcg agcttcgggt gatgtacgag
     5401 caccacgatg cgatcctgca ttcggtgtcc gagggactgg tcgtgctcga ccgcaacggg
     5461 gtggcgctcg tcaacgacga ggcgcggcga ttgctcggcc ttccgcccgg tcccgtcgcc
     5521 acctcggact tgcccgagtt cctgcgcacc tacaaccctg gtgcgcgcga cgaggtgcat
     5581 gtcaccgacg aacgcgtgct cgtggtcaac cgctctcagg tggacaaccg gccgcaagac
     5641 tctgaagtgg tgaccatccg cgaccgcacc gaattacagg gcgcgctggg cgaactgagc
     5701 tcgctgcagg ttctcaccga ctcgctgcgc tcgcaggcgc acgaggccgc caacaagctg
     5761 catacggtga tcaccatggt cgagatgggt cgcgccgacg aagcggtcag gttcgccacc
     5821 catgaactcc agctgtccca gcaattggtc gatcgtctct cggccgatgt cgccgaaccg
     5881 gcgcttgtcg cactgcttct cggcaaggcc gcgcaggccg acgaacgcgg catcgagttg
     5941 tccgtcaccg aggacaccca tctgccgtcg aacatcgatg acgtgccact gtccggccag
     6001 gagatggtga ccgttctcgg caacctgatc gacaacgcga tggatgcgtg cgatcgcgac
     6061 aacccgtggg tcgaggtcac ggtcagtcag gacgaccaca ccctgctgct ccgggtggcc
     6121 gacagcggct ctggcatgga ctccgccgcc ttcgagaagg ccatgcagcg cgggtactcc
     6181 accaaatcgg gcgtcgacac cggccagcac gggctcggat tggccctcgt cgcgcaggtc
     6241 gtcaagcgtc acaacggaac cctgaccgcc gacgtgacat acggttcagt ggtgacggtc
     6301 acggtgagtg actcgtgatc agcgttctga tcgtcgagga cgagccgttg atcgccgagg
     6361 cgcatcaagc gtacttgcaa cggctggaag gattttcggt cacggcggtg acccacacgg
     6421 ctcgcgacgc gatgagagcc gctgccactg cagcggcgaa cgagtcgccg atcgacctgg
     6481 tgctgctgga catcgggctt cccgacgcga gcggcatctc actggcgtcc ggtttgtcgg
     6541 gtctgcgacc ggcaccggac atcatcgcga tcacgtccga acgcgatctg gagatggtgc
     6601 gcgccgcggt cggccatggc gcactggcgt acctgctcaa gccgttcacg ttcgcggcgt
     6661 ttcgcgaccg gttggagcgc taccagcgct accgcgaggc gttgcccgcg gggaccgacg
     6721 cggccagcca ggccgaagtc gaccgtgcgc ttgccgaatt gcgggtgagt accgaccgat
     6781 ccgcggctcc gaagggggcc gccgcgggca ccaacgacga gatcgcccgt gcggtaaggg
     6841 attcgcccga cgggatcacc gccgacgcgg tggccaagca ggtcggagtc tcccgtgtca
     6901 cggcgtggcg ctacctcgag cgcctcgccg acgagggcac cgtcacccgg cacaccgact
     6961 acggcagggc cggcaggccg aaaacccgct accagtggcg ctaagacccc agccggtgga
     7021 catcggtgtc gcgcggcggt cagccgtggc gaatcatctc cgcgccacgt tcggcgatcg
     7081 cgtacacggt ggccagcgtg ttgttcgacg gcagcgacgg catgaccgag gcgtcgacga
     7141 cccgtaaacc gtcgatgccg tgcacacgta gttcactgtc gacgaccgac cgtccgctct
     7201 gcccgatcgc gcatgtgccc accgggtggt agtacgacga cgtcgttgcg cgcacatact
     7261 cacgcagcga ctcttcatcg tcgacggccg gccccggcgc caattcctgt gcgcaccagt
     7321 cgtccaacgc atgtgcggac ccgatatggc gggcgatccg gattccctcc agcatcgtat
     7381 gcatgtcgcg gtcatcgccg aggtagttcg ggtcgacgac cggtggaaca cccggattcc
     7441 aggacttcag ccgaacggtg cccctgctgt gaggttgcaa ggcagaaacg ccgatcagat
     7501 aggtgtcggg gacgtcgagc ccgataaccg aagc
//
LOCUS       DASYLX010001105         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3702399,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001105 DASYLX010000000
VERSION     DASYLX010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3702399"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGP27_25875"
     CDS             <1..651
                     /locus_tag="VGP27_25875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7854765.1"
                     /translation="QDHWAVRSQNRAEEAINSGFFAREISPVTLPDGSQVSTDDGPRA
                     GTTYEKISQLKPVFRPNGTVTAGNACPLNDGAAAVIVTSDEKAKELGLTPLARIVATG
                     VSGLSPEIMGLGPIEATRKALANAGMTIDDIDLYEINEAFAVQVLGSARELGMDHDKL
                     NISGGAIALGHPFGMTGARITATLLNNLTTQDKTFGLETMCVGGGQGMAMIIERLS"
     gene            complement(689..1552)
                     /locus_tag="VGP27_25880"
     CDS             complement(689..1552)
                     /locus_tag="VGP27_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEV7854766.1"
                     /translation="MLVGPFLGSEAIERRLVRKHELRSRYRNVFPDVYVSKDAALTLD
                     QRAKAGWLWSHRQGIIAGLTASALHGAKWVDDDAPVELIWPNARPARGLRTYDYRLRT
                     DESGVRDGMRVTTPARTGFDVGRRRPLDTAIANVDALGNATGLTAAEIFRVARRHRGS
                     RGLRQLADVLDLYDPGAASPKETWLRLLVIRADFPRPRTQIPVLSADGARRYYLDMGW
                     EHLMLAIEYDGEQHRIDSVQYAYDIRRSEDLSELGWTRLRVVKTNTAGDVVRRLDRAW
                     RSKLRGDREFQ"
     gene            complement(1700..2554)
                     /locus_tag="VGP27_25885"
     CDS             complement(1700..2554)
                     /locus_tag="VGP27_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV7854767.1"
                     /translation="MRESSNPVFRSLPKTQGGYAQFGTGATGAGAAAVHADPYVTQYP
                     DRQQTGVSRPMTIDDVVTKTGVTLAVLSAVAVVSYFLVAGNTALAMPFVLVGGLGGLA
                     LVLIATFGRKQDKPAIVLGYAALEGLFLGAISFVLANFYVSGASAGALIGQAILATIG
                     VFVGMLVVYKTGAIRVTPKFTRMIVAGIFGVLVLALGNFVLAMFGVGGGEGLGLRGNG
                     PIAIIFSLVCIGLAAFSFLLDFDAADQMIRAGAPEKAAWGVALGLTVTLVWLYIEILR
                     LLSILQSD"
     gene            2711..>3150
                     /locus_tag="VGP27_25890"
     CDS             2711..>3150
                     /locus_tag="VGP27_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7854768.1"
                     /translation="MGENEDVLVTVDRGTGLISLNRPKAINSLTHSMVSAVSEALTEW
                     RDDGAVRVVVLSGQGERGLCAGGDIVDVVYKSARGDHGIASSFWWDEYLMNAQIARYP
                     KPFVSLMDGIVMGGGVGVSAHGSVRVVTDTTKMAMPEVGIGFIP"
BASE COUNT          611 a         1000 c         1020 g          519 t
ORIGIN      
        1 caggaccact gggccgtgcg cagccagaac cgtgccgagg aagcgatcaa cagcggcttc
       61 ttcgcccgcg agatatcgcc ggtgacgctg ccggacgggt cgcaggtcag caccgacgac
      121 ggaccacggg cgggcaccac ctacgagaag atcagccagc tgaagccggt gttccggccg
      181 aacggcacgg tcaccgcggg caatgcctgt ccgctgaatg acggtgcggc agcggtgatc
      241 gtgacgagcg acgagaaggc caaggagctc gggctgacgc cgctggcgcg gatcgtcgcg
      301 acgggcgtga gtggcctgtc gccggagatc atgggcctcg gaccgatcga ggcgacgcgc
      361 aaggcactcg ccaacgcggg catgacgatc gacgacatcg acctctacga gatcaacgag
      421 gcgttcgcgg tgcaggtgct gggttcagca cgtgaattgg gcatggacca cgacaagctc
      481 aacatctccg gcggcgcgat cgcgctgggg cacccgttcg gcatgacggg cgcgcgcatc
      541 acggccacgc tgttgaacaa cctgaccacg caggataaga cgttcggcct ggagacgatg
      601 tgcgtcggcg gaggccaggg aatggcgatg atcatcgagc gcctttcctg atctttccaa
      661 gcgccgaaac tgcggtgaga tcgcaatctc actggaattc gcgatccccc cgcagtttcg
      721 agcgccatgc gcggtcgagt cggcgtacga catccccggc ggtattcgtt ttgacgaccc
      781 tcagccgcgt ccatcccagt tcgctgaggt cctccgagcg ccgtatgtcg taggcgtatt
      841 gaacggagtc gatacggtgt tgctcgccgt cgtattcgat ggccagcatc aagtgctccc
      901 aacccatgtc gaggtagtac cgtcgagcgc cgtccgcgct caacaccggt atctgcgtcc
      961 gcggccgcgg gaagtctgcc cgaatgacca agagccgcaa ccacgtctcc ttcggtgatg
     1021 ccgcccccgg gtcatacaga tccagtacgt ctgcgagctg acgaagcccc cgcgatccgc
     1081 gatgacgtct tgcgacgcgg aaaatttcgg cggccgtcaa ccctgtggcg ttgccgagtg
     1141 catcgacgtt ggcgatcgcc gtatcgagcg gtctacgccg gccgacgtcg aaacctgtac
     1201 gcgcaggagt cgtcacacgc attccgtcgc gcacaccgga ttcatctgtc cggagccgat
     1261 agtcgtaggt gcgcaatccg cgggcgggtc gggcattggg ccatatcaac tcgacgggcg
     1321 cgtcatcgtc gacccatttc gcgccatgta gggcggacgc ggtcagcccg gcgatgatgc
     1381 cctgcctgtg agaccacagc caacctgcct tggctcgctg atccagcgtc agcgcggcat
     1441 cctttgacac gtagacatcg gggaagacat tgcgatagcg ggaccgcagc tcgtgctttc
     1501 gaacgagcct gcgctctatt gcttcactgc cgagaaacgg cccgacgagc atggacatga
     1561 ctcgactctc gcgggtcgac gtcgccgagc ctgcgggcag atcgcgatct gtggacgaaa
     1621 tgcgatctgt gagcagtttc ggcgggagag cgcaaaaaga aagcccccgg catgccgggg
     1681 gctttcgtgt agtggatcgt caatcgcttt gcaagatgct gagcagacgc aagatctcga
     1741 tgtagagcca gaccagtgtg acggtcaggc caagcgcgac accccatgcg gccttctccg
     1801 gcgcgcctgc gcggatcatc tgatcggcgg cgtcgaagtc gagcaggaag ctgaacgccg
     1861 ccaggccgat gcacaccagc gagaagatga tcgcgatggg gccgttgccg cgaaggccga
     1921 ggccttcacc gcctccgaca ccgaacatcg ccagtacgaa gttgcccagc gccagaacga
     1981 gcacgccgaa gatgccggca acgatcatgc gggtgaattt cggcgtcacg cggatcgcgc
     2041 cggtcttgta gacgacgagc atgccgacga acacgccgat ggtggcgagg atcgcctgcc
     2101 cgatgagggc accggcgctg gcgccggaca catagaagtt ggcgagcacg aacgagatcg
     2161 caccgaggaa caatccctcg agtgcggcgt agccgagcac gatcgccggt ttgtcctgct
     2221 tacggccgaa cgtggcgatg agcacaagcg ccaggccgcc gagcccgccg accaagacga
     2281 acggcatcgc cagcgcggtg ttgcccgcga ccaggaagta cgagacgacc gcgacggccg
     2341 acaacaccgc aagcgtgaca cctgtcttgg tgacgacgtc gtcgatggtc atcggacgcg
     2401 aaaccccggt ttgctgccgg tcggggtact gcgtcacata cggatcggca tggactgcgg
     2461 ccgcaccggc gcccgtggct ccggtaccga actgcgcata accaccctgc gtcttaggca
     2521 gggatcgaaa taccgggttg ctgctctcgc gcaccgtcgg ttcctctcct gtgctgtcgt
     2581 tcgcctgctg acgaacacct attcaacgat cggccactgg taccggttcc cgagaaacgc
     2641 tcccagctcc ttcacaggaa agactacccg gggcgggcca tctgagggca acgttctaca
     2701 ttgcataacc gtgggcgaaa acgaggatgt cctagtaacc gtcgaccgcg gaaccggcct
     2761 gatcagcctg aaccgaccca aggcgatcaa ctcattgacc cattcgatgg tctcagcggt
     2821 ctctgaggcg ctgaccgaat ggcgggacga cggcgccgtt cgcgtcgtgg tgctctccgg
     2881 tcagggcgag cgcggcctgt gcgcgggcgg cgatatcgtg gacgtggtct acaaaagtgc
     2941 ccgcggcgac cacggcatcg ccagctcgtt ctggtgggac gagtacctga tgaacgctca
     3001 gatcgcgcgc taccccaagc ccttcgtctc gttgatggac ggcatcgtga tgggcggcgg
     3061 cgtcggcgtc agcgcacacg gcagcgtccg cgtcgtcacc gacaccacga agatggcgat
     3121 gcccgaagtg ggtatcgggt tcatccccga
//
LOCUS       DASYLX010001106         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1179478,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001106 DASYLX010000000
VERSION     DASYLX010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179478"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGP27_25895"
     CDS             complement(<1..210)
                     /locus_tag="VGP27_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854769.1"
                     /translation="MARRPDVERRRQLLDALIDAFADGGIGDRSLREIAEVVGTSHRM
                     LLHHFRSRDELLLSIVDEVERRQMSV"
     gene            complement(194..628)
                     /locus_tag="VGP27_25900"
     CDS             complement(194..628)
                     /locus_tag="VGP27_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7854770.1"
                     /translation="MITDSAIEIGAPAALVWDVFADVERWPEWTASVTTLTALDGSGL
                     AVGRRFAIKQPRLPKLVWEVTALDEGKSWTWEQRSPGGRTVAVHEVEAQGGRTLVRQR
                     LDQQGPVGSLVARLMGGTTKRYLELEAQGLKARSEQRSGSAS"
     gene            complement(958..>1934)
                     /locus_tag="VGP27_25905"
     CDS             complement(958..>1934)
                     /locus_tag="VGP27_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV7854771.1"
                     /translation="LDNARRGRLGLSVADYRAAMAELFAPLSKTAAKNPFSSSPVERS
                     VDEIITVTDDNRMICDPYPRLLVARDQVNQGAAAVMMSVDAARRLGVPEEKWVYLHGH
                     SDMVEQGLLERVDLAASPATVHAAREALRVAEIGVDDVATFDLYSCFPFPVFVMCEAL
                     GIDADDPRGLTLTGGLPYFGGPGNSYTLHAIAETVAEMRDKPGQFGLVGANGGTMSKY
                     SVGIYSALPVEWRSDRSAALNEEVAAFPSIAVTEKADGPATVETYSVRYDWPVRTGVI
                     IGRLDADDSRFMALADDEDLVGLMSDGDPLGAHIVVTSTEQGNRAHLA"
BASE COUNT          332 a          646 c          638 g          318 t
ORIGIN      
        1 cacggacatc tgcctgcgct cgacctcatc gacgatcgac agaagcagtt cgtcacggga
       61 tcggaagtgg tgcaacagca ttcggtgact tgtgccgacc acttcggcga tctcacgaag
      121 cgagcggtcg ccgatgccgc cgtcggcgaa ggcgtcgatg agggcgtcaa gcagttgcct
      181 tcgccgctcg acgtcaggac gccgagccac tgcgttgctc actacgcgcc ttgaggccct
      241 gcgcttcgag ttcgagatag cgcttggtag tgccgcccat cagccgggcg acaagagatc
      301 cgaccgggcc ctgctggtcg agccgttgcc ggaccagcgt gcggccgccc tgtgcttcga
      361 cctcgtgtac ggcgacggtc cggccgccgg gcgaccgctg ctcccacgtc catgatttgc
      421 cctcgtccag cgcggtgact tcccacacca gcttcggcag acggggctgc ttgatcgcga
      481 accgcctccc caccgcgagg ccggacccgt cgagggccgt caaggtcgtc accgacgccg
      541 tccactccgg ccagcgctcg acgtcggcga agacatccca caccagggcg gcgggggcgc
      601 cgatctcgat ggcactgtcg gtaatcatgt accagatggt acaagaatgc gcaggtgatg
      661 tgaagtagtg cacgcagcgg cattcgcgta gtcgactacg cgtgccgtgc ccggcgcacg
      721 ccgcgagagt cgtaggcacg agagcgtccg catgacacaa cgagagggag tctcatgcct
      781 tccatcagcc cactgattgc ccgcgtattc gacgatccgc agccaaaccg tcgtcacagg
      841 cgtgaccgcg aaggcttccg cagccatgcc gagaccaagg gccgggtggc gcgccgtcga
      901 tcacgctgac gcgggccccg cgatagtcga tctcgacctt cgactgtttg tgcgggacta
      961 ggccaggtgc gcgcggttgc cctgctcggt agaggtcaca acgatgtgtg caccgagcgg
     1021 atcgccgtcg gacatcaagc cgacgagatc ctcgtcatcc gccagcgcca tgaaccggga
     1081 gtcgtcggca tcgagcctgc cgatgatgac cccggtgcgg accggccagt cgtagcgcac
     1141 agagtaggtc tcgacggtcg cggggccgtc agccttttcc gtcacggcga tcgaggggaa
     1201 cgccgcgacc tcttcgttga gggccgcact gcggtcggaa cgccattcga ccggcagggc
     1261 cgaatagatg ccgacggagt acttgctcat ggtgccgcca ttggcgccga cgaggccgaa
     1321 ttggcccggc ttgtctcgca tttcggctac cgtctcggcg atggcgtgaa gtgtatagct
     1381 gttgccgggg ccgccgaaat agggcaagcc tcccgtcagt gtcaacccgc gcggatcgtc
     1441 cgcgtcgatt ccgagggcct cgcacatgac gaacaccggg aacgggaagc agctgtacag
     1501 atcgaaggtg gcgacgtcgt cgacgccgat ttccgcgacg cgcaacgctt cccgtgcggc
     1561 atgcaccgtc gcgggactgg cagcgaggtc gacgcgctcc agcagcccct gttcgaccat
     1621 gtcggagtgg ccgtgcagat acacccactt ctcctcgggc acaccgaggc gacgcgcagc
     1681 gtccaccgac atcatgaccg ccgcggcgcc ctggttgacc tggtcgcggg cgacgagcag
     1741 ccgcgggtag gggtcgcaga tcatccggtt gtcgtcggtg accgtgatga tctcatccac
     1801 cgagcgttcg acgggcgacg acgagaatgg gttcttcgca gcggttttgg agagcggcgc
     1861 gaacagctcc gccatcgccg cccggtagtc ggcgacgctc agcccgagcc ggccccgacg
     1921 cgcgttgtcc agca
//
LOCUS       DASYLX010001107         4843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_742940,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001107 DASYLX010000000
VERSION     DASYLX010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742940"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VGP27_25910"
     CDS             complement(<1..1205)
                     /locus_tag="VGP27_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7854772.1"
                     /translation="MDSAQAFELAEIDAVGQGALVTAGELSAVELLDAAITRLEAARD
                     LNAVVHDLFDRGREQAAELDASGRARAAESGPLAGVPFLLKDLGASLAGAPEAMGSRA
                     LRTHVATETAWIVDRYLSAGLVVFGKTNTPEWGNHCTTEPSLFGPTVNPWSPSITPGG
                     SSGGSASAVAAGVVPAASGGDGTGSIRVPASCCGLVGLKPRRGRSSFAPDSGHSLEGL
                     VNAHALTRTVRDSAALLDVIAGSAPGDPYTAAPPATTYLDAITKDPPPQRILLVTASP
                     FPGPPTDPAVITAVENTAQLLGEIGHFVEPGFPAIDSDAVADAIAILHTVSNAALHAL
                     ATNHLGREPAEDEFEPSTWVMVREGFNTTGAAYAGAIDAVHAQTRRFAAGMAGHDVLL
                     VPTLLTPPPP"
     gene            1311..2087
                     /gene="fabG"
                     /locus_tag="VGP27_25915"
     CDS             1311..2087
                     /gene="fabG"
                     /locus_tag="VGP27_25915"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEV7854773.1"
                     /translation="MFTLAGKSVVVTGGSKGIGRGIASVFAGAGANVAVAARSAADLD
                     AAVAALDGLGDGKVVGVTTDVSEPASCTAMAAAVVDAFGGIDVLCANAGIFPEAPLAT
                     MTPEELAEVLDVNVKGTVFAVQACLPALIDSGCGRVILTSSITGPITGFPRWSHYGAS
                     KAAQLGFMRTAAIELAPHAITVNAVLPGNILTEGLADMGEDYLAGMARAIPAGALGRP
                     ADIGYLAAFLATDEAGYITGQAIAVDGGQVLPESPDAVTP"
     gene            complement(2171..3637)
                     /locus_tag="VGP27_25920"
     CDS             complement(2171..3637)
                     /locus_tag="VGP27_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEV7854774.1"
                     /translation="MTTVGQTDEKPAGRTLFGHPIGLANLFGVELWERFSFYGMLTIL
                     GYYLYYSVTEGGLELPKTTATGIVGAYGGLVYLSTVLGGWVADRVLGMERTVFYGGVV
                     VMCGHIALAIIPGLAGVAVGLVLVALGSGALKANASSLLGTLYEKDDPRCDGGFTLFY
                     LGINLGAFVGPLVTGLLQTRIGFHWGFGAAAVGMALGLAQYVAFRKNLGEHGRNVPNP
                     LPRSGIKWAVIAAVVVVVVVVVAFVTGLVKLANLSQVTTGVIIVASIAYFVIMLTSSK
                     VELVERTRVRAFIPLFIANAVFWSLFQQIFTVLAVYSDERMNWSIFGWTAPSSWIGSI
                     EPVWIILLSPLFAIMWTKLGNRAPTTPRKFAYGVIGMGAAFLLFLPMAGTEGEAVPAL
                     YVAAVLAVFAVSELLLSPIGLSVTTKLAPEAFRAQMMALYFFSVGLGTSMSGVLAKYY
                     DAAREFAYFGIIGVVAIVAGVVVFALAPWISRKMEGVH"
     gene            3743..4039
                     /locus_tag="VGP27_25925"
     CDS             3743..4039
                     /locus_tag="VGP27_25925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854775.1"
                     /translation="MAFKVRFAGQPQPQDYGDDDGFEFLDGGVLAIHFGDEETWAEYY
                     PPSKWEQVTADPDHLAGLNTPYVDEEDEDYDEDEDYEDEVDEDEVDEEPEPEDE"
     gene            complement(4334..>4843)
                     /locus_tag="VGP27_25930"
     CDS             complement(4334..>4843)
                     /locus_tag="VGP27_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7854776.1"
                     /translation="RRVWAGEKVTDSVLPVGPAPVQEAGPRLLVGTTGPKTVQSAAAW
                     ADGLAGTTLDLDTAKQNELYDVARTAWARAGKPKPHLATSFWFAIGDGESARAQVHRH
                     LRRYMNWIPIEYVDAMAPSTGWAGTEDQLASVLQQFDDIGTDEVHLIPTSSDVEQVRR
                     VADVVKDFG"
BASE COUNT          872 a         1600 c         1608 g          763 t
ORIGIN      
        1 ggcggcgggg gcgtcagcaa tgtcggcacg agcagcacgt cgtggcccgc catgccggcc
       61 gcgaagcgtc gcgtctgggc atgcaccgcg tcgatggcac cggcatatgc ggcgcccgta
      121 gtgttgaacc cctcacggac catcacccac gtgctcggct cgaattcgtc ttccgcaggt
      181 tcacggccga gatgattcgt cgccaacgcg tgaagcgcgg cgttgctgac ggtgtgcaga
      241 attgcgatcg cgtcggcgac ggcgtcggaa tcgatcgcgg gaaagcccgg ttcgacgaag
      301 tgaccgattt cgccgagtag ctgtgcggtg ttctcgacgg cggtgatgac agcgggatcg
      361 gttggcggac ccggaaaagg cgacgcagtc accagcagaa tccgttgtgg cggtgggtct
      421 tttgtgatgg catccaggta ggtggtggcc ggcggagcgg cggtataggg gtcgccgggg
      481 gcgctgcccg cgatgacgtc gagcagggcg gcgctgtccc gcacggtacg ggtgagcgcg
      541 tgtgcgttga ccaaaccctc cagtgagtgg ccggagtccg gcgcgaacga gctccggccg
      601 cggcgcggtt tgaggccgac gagtccgcag cacgacgcgg gcacccggat cgagccggtg
      661 ccgtcgccac ccgacgccgc ggggacaacg cctgcggcta cagccgacgc cgatcccccg
      721 ctcgacccac ccggcgtgat cgacggcgac cacggattga ccgtcgggcc gaagagcgac
      781 ggctcggtgg tgcagtggtt gccccactca ggggtattcg tcttaccgaa gaccaccagg
      841 cccgccgaca ggtaacggtc cacgatccaa gccgtttcgg tcgcgacatg agtgcgcaga
      901 gcgcgcgaac ccatcgcctc cggcgcccct gcgagcgacg cgccgagatc cttcagcagg
      961 aacggcaccc cggccagcgg acccgattcg gccgcgcgcg cccgacctga ggcgtccaac
     1021 tcggcggcct gctcacgccc acggtcgaac aggtcgtgga cgacggcgtt gaggtctcgc
     1081 gcggcctcga gccgcgtgat cgccgcgtcg agcagctcaa ccgcggacaa ttcaccggca
     1141 gtgaccagtg cgccctgccc gacggcgtca atttcggcca gttcgaaggc ttgtgcggag
     1201 tccattgatt gggaatcctc gcgcctcacc accggaccgg caacccggcc gcgcataaca
     1261 gtctctgact ccgactgcgc ggcggccggt gctgctccac actgagtgct gtgttcacac
     1321 ttgctggaaa gtccgtcgtc gtcaccggcg gcagtaaagg tatcggtcga ggtatcgcct
     1381 ccgtgttcgc cggagccggc gcgaacgtgg cggtggccgc acggtcggcg gctgacctcg
     1441 atgcggcagt agcggctctg gacggtctcg gcgacggcaa ggtggtcggc gtgacgaccg
     1501 acgtcagcga acccgcctca tgcacggcga tggccgccgc ggtcgtcgat gccttcggcg
     1561 gcatcgatgt gctgtgcgcc aatgccggca tttttcccga ggcgccgctg gccacgatga
     1621 ctcccgagga gctggccgag gtgctcgacg tcaatgtcaa gggcacggtg ttcgcggtac
     1681 aggcgtgcct gcccgcgttg atcgactcgg gctgcggccg cgtcattctg acgtcgtcaa
     1741 tcacgggtcc catcacagga tttccccgat ggtcccacta cggcgcgtcg aaagctgcgc
     1801 aactcggttt catgcgcacc gccgcgatcg aactcgcgcc ccatgcgatc accgtcaacg
     1861 ccgtcttgcc gggcaacatc ctcaccgaag gcctcgcgga catgggcgag gattatctcg
     1921 caggcatggc acgggccatc cctgccggcg cgctgggcag gccggctgac atcggctacc
     1981 tcgcggcgtt ccttgcgacg gacgaggccg ggtacatcac cggccaggcg atcgcggtgg
     2041 acggcggaca ggtgctgccg gagtcaccgg acgccgtcac gccgtagacc ggggcggccc
     2101 ggccgaagca cgagcactgc cgcatggcag gaaatcgcct gtgggtgcga tcctgccatg
     2161 gacccgtccc tcagtgcacg ccctccatct tgcggctgat ccacggcgcg agagcgaaca
     2221 ccaccacacc cgcgacgatc gcaaccacgc cgatgatccc gaagtaggcg aattcacgcg
     2281 cggcgtcgta atacttggcg agcaccccgg acatcgatgt gccgagaccg accgagaaaa
     2341 agtacagcgc catcatttgc gcccggaatg cttccggcgc aagctttgtc gttaccgata
     2401 gcccgatcgg cgacagcaac agctcggaaa cggcgaacac cgccaacacc gcggcgacat
     2461 acagcgcggg caccgcctcg ccctcggtgc ccgccatcgg aaggaacagc aggaatgcgg
     2521 cgcccatgcc gatcacgccg taagcgaact tgcgcggcgt cgtcggcgcc cggtttccga
     2581 gcttcgtcca catgatcgcg aacagcggcg agagcaggat gatccacacc ggctcgatgg
     2641 agccgatcca gctcgacggc gccgtccacc cgaagatcga ccagttcatc cgctcgtcgg
     2701 agtacaccgc gaggaccgtg aagatctgct ggaacagcga ccagaacacc gcgttggcga
     2761 tgaacagcgg aatgaacgcc cgcaccctgg tgcgttcgac cagttcgact ttcgagctgg
     2821 tgagcatgat tacgaagtac gcaatcgacg ccacgatgat gacgcccgta gtgacctgtg
     2881 acaggttcgc aagtttgaca aggcccgtga cgaaggcgac cacgaccacg acgaccacca
     2941 cgacggcggc aatgacggcc catttgattc cactgcgcgg caatgggttc ggcacgttac
     3001 gaccgtgctc gccgaggttc ttgcgaaagg cgacatactg cgccaggccg agcgccatcc
     3061 cgacggctgc ggcgccgaag ccccagtgga agccgattcg cgtctgcagc aggccggtga
     3121 ccagcggacc gacgaacgcg ccgaggttga tgccgagata gaacaatgtg aagccgccgt
     3181 cacaacgcgg gtcatctttc tcgtacagcg tgccgagaag agatgatgcg ttggccttca
     3241 acgcgcccga gccgagcgcg acgagcacca ggcctacggc gacacctgcc aggcccggaa
     3301 tgatcgcgag cgcgatgtgg ccgcacatca cgaccacgcc tccgtagaac acggtccgtt
     3361 ccatgccgag gacgcggtcg gcgacccagc cgccgagcac ggtggagagg tacaccaggc
     3421 cgccgtaggc cccgacgatg cccgtcgcgg tggtcttcgg cagttccaga ccgccttcgg
     3481 tcaccgagta atagaggtag tagccgagga tcgtgagcat cccgtagaac gagaaccgct
     3541 cccacaactc gacgccgaag aggttggcga ggccgatcgg gtgaccgaac agggtgcgtc
     3601 cggccggctt ctcgtccgtc tgtcccactg ttgtcatgag ggccagaatt accacgtgcg
     3661 cctgcgctcg gggaaggggc gccggaggca tcgcaggtag gccgtccagt cgcgtcaccc
     3721 ccgtcatgga aaactggccg ccatggcgtt caaggtgcga ttcgccgggc agccccagcc
     3781 ccaggactac ggcgacgacg acggcttcga gttcctggac ggcggcgtgc tcgccatcca
     3841 cttcggtgac gaggagacgt gggccgagta ctacccgccc tcgaaatggg agcaggtgac
     3901 ggcggacccc gatcacctgg cggggctgaa caccccgtac gtcgacgaag aggacgagga
     3961 ctacgacgaa gacgaggatt acgaggacga ggtcgacgag gacgaggtcg acgaggaacc
     4021 cgaacccgag gacgagtagt cggctcaggc tcgagcgcgg attcactctg cggtgagggc
     4081 gaccgctact cgcacttctg tgccgtattc gcagagtcaa agccgctgac acgttcgggc
     4141 agtgcgcacc acgttcgagt tcgacttctt gagaactcga gccccgacga gcggcgacac
     4201 cgacgcggag atccgtgtgg gccaggattt cccgtagccc tgggcgcgat ggcgttacga
     4261 ctggtccgct cccgcctcgg tgacgctgac cttggtgcag tgcacgccgt gtgggatcag
     4321 aactacacgc cccctacccg aaatctttga cgacgtcggc tacccgccgc acctgttcca
     4381 cgtcggagct ggtcgggatg aggtgaacct cgtcggtgcc gatgtcgtcg aattgttgca
     4441 gcacacttgc caattggtcc tcggtgcctg cccatccggt gctgggcgcc attgcgtcga
     4501 cgtactcgat cgggatccag ttcatgtagc ggcgcagatg ccggtgcacc tgtgcccgcg
     4561 ctgactcacc gtcgccgatg gcgaaccaga acgaggtggc cagatgcggt ttgggcttcc
     4621 cagcccgtgc ccacgctgtt cttgcgacgt cgtagagctc gttctgcttg gcggtgtcga
     4681 gatcaagtgt ggtgcccgcc agaccgtcgg cccatgcggc ggcggactgc accgtcttgg
     4741 gacccgtggt tccgaccagc agtcgcggac cggcctcctg cactggcgcg ggaccgaccg
     4801 gaagcacgga atcggtcacc ttctcgccgg cccagactcg tct
//
LOCUS       DASYLX010001108         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2168973,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001108 DASYLX010000000
VERSION     DASYLX010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168973"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1476)
                     /locus_tag="VGP27_25935"
     CDS             complement(205..1476)
                     /locus_tag="VGP27_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV7854777.1"
                     /translation="MQDDLPAPAISRSVRVLGPVVLLVALALYLAAYLRWPSLASQVD
                     LLVYRFGAMRVWDGQDLYSVGMTGNSHTMLFDYTPFAALCFLPSIGLSQPAILTLGFL
                     VNMACVGYVVRRMLSAAGITTATGLWGLTALLVAPIIWLEPVRLSLQLGQINLLMMAI
                     VLADVLSQPSRRWTGIGIGIAAGIKLTPALFILFLVATGRRREAVIAGLTFISTIAVG
                     FVLLPADSNEFWLGGRFQDVRRISSDPLANTSLAGLLARLNWATRWETAAAVVFAIFA
                     IAIAAVAWRRGHLLLGVAIAGMTSAAASPFSWSHHWVWFAPLLVHLGLLGYVHRSRAA
                     ALAMWTLAAIFGGWFIASRSSPPQAGLMSLRHPGTWNQLLPAAYLFALTVVLIFSVYT
                     FWRARQHPAATTIGFDVIVEGSRPRASIGPI"
     gene            complement(1738..1811)
                     /locus_tag="VGP27_25940"
     tRNA            complement(1738..1811)
                     /locus_tag="VGP27_25940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1777..1779),aa:Gly,seq:ccc)
     gene            1870..2442
                     /gene="dcd"
                     /locus_tag="VGP27_25945"
     CDS             1870..2442
                     /gene="dcd"
                     /locus_tag="VGP27_25945"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV7854778.1"
                     /translation="MLLSDRDLRSEIAAGRLGIDPYDESLVQPSSVDVRLDNLFRVFN
                     NTRYTHIDPSVRQDELTTLVEPKEGEPFVLHPGEFVLGSTLERCTLPDDLAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCLLRLTSPAEN
                     PYGSAKAGSKYQGQRGPTPSRSYQNFIKSN"
BASE COUNT          604 a          865 c          846 g          442 t
ORIGIN      
        1 tggcctactc aacttttccg accttgcgtg aaagttgcgc ccgcaccgcg gccaccgcct
       61 ccgctaacgc gccaccggag gggtagcgca tcgccgcagg aaacgtggag ccgatgacgg
      121 gaatcgaacc cgcgtattca gcttgggaat cggttggggc agggccgccg tccaggaaaa
      181 cccgacaacg gctaatgatc acgcttagat cgggccgatg ctcgctcttg gacgactgcc
      241 ctccactatt acgtcaaagc cgatcgttgt ggccgctggg tgctgtcgtg cccgccagaa
      301 tgtgtacacg ctgaagatga gcacgacagt aagcgcgaaa aggtaagccg cgggaagaag
      361 ctgattccac gtccccgggt ggcgcaagga catcagacct gcttgtggag gcgagcttcg
      421 cgatgcgatg aaccaccccc cgaagatcgc cgcaagcgtc cacatggcca gggccgcggc
      481 gcgcgagcgg tgtacgtacc cgagaagccc aaggtggaca agaagcggtg cgaaccacac
      541 ccaatggtgg ctccagctga acggtgaagc ggcggccgag gtcatccccg cgatggcgac
      601 gcctaagagc agatgaccgc gacgccacgc caccgcggcg attgcgatgg cgaagatcgc
      661 gaatacgact gcggccgcgg tctcccaccg cgtggcccag ttgagccgcg cgaggagtcc
      721 agccagactg gtgttcgcca gtgggtcgct ggagatccgt cgtacatcct ggaaccgccc
      781 ccccagccag aactcgttcg aatcggccgg cagcagaacg aaaccgacgg cgatggtcga
      841 gatgaacgtc aatccagcga tgactgcctc gcgacgtcgc ccggttgcga cgagaaagag
      901 gatgaagagc gcgggagtca gtttgatccc ggcggcgatc ccgataccta tcccagtcca
      961 cctgcgggac ggttgcgaca gcacgtcagc gaggacgatc gccatcatga gcaaattgat
     1021 ctggccgagc tgcagcgaaa gccggacagg ctccagccag atgatcggag ccaccagaag
     1081 cgccgtcaac ccccacagac cggttgccgt cgtgatccct gcagcactga gcatccggcg
     1141 gacgacgtaa cccacgcagg ccatgttcac caaaaaacca agggtgagaa tggcaggctg
     1201 gctcaacccg atcgacggga gaaagcacag cgcagcgaat ggcgtgtagt cgaacagcat
     1261 cgtgtgtgaa tttccggtca tccccactga gtacaggtct tgaccatccc aaacgcgcat
     1321 tgctccgaac cggtacacca ataggtcgac ctggctggcc aggctgggcc accgcagata
     1381 tgcggctagg tacaacgcca acgcaaccaa taagacgacc ggccccaaca cacggaccga
     1441 tcgagagatg gcgggcgcgg gaagatcgtc ctgcacgata agccgactcc ttcatcggtg
     1501 tccgtactcg ttgccgtggc agttacggtg cgcgaggata gcggtcaacc ctgaaagcgc
     1561 cccacctggc cgacaactaa gcagcgaagt cgggagacgc acgacatctt ctacccggct
     1621 cagcgcccgg caaacgttcg cagtggctat ctgccctcgc cacggtcaaa cgacggtaaa
     1681 agataaattt tgacgggcgt cggcagtcgc ggaatatgcc gccgaactgc ggaactgtgg
     1741 agccgatgac gggaatcgaa cccgcgtatt cagcttggga agctgatgtt ctgccattga
     1801 actacatcgg cctgcccggc tggtgccggt gatcacgaag gctaacactc cagcggttac
     1861 gctcgtcggg tgctgctctc cgatcgcgac ctccgcagcg agatagccgc gggacgtctt
     1921 ggcatcgatc catacgacga gagcctggtg cagccgtcga gcgtcgatgt ccggctggac
     1981 aacctgtttc gggtattcaa caacacccgc tacacccata tcgacccgtc ggtgcgccag
     2041 gacgagctca ccaccctggt ggagccgaag gagggcgagc ccttcgtact gcatcccggc
     2101 gagttcgtgc tcggctcgac cctggaacgg tgcacccttc ccgacgacct cgccggccgc
     2161 ctcgagggca agtcatcgct tggccggctc ggcctgctga cacactcgac cgcgggcttc
     2221 atcgacccgg gcttcagcgg gcacatcacc ctcgagctgt ctaacgtcgc gaacctgccg
     2281 atcaccttgt ggccgggcat gaagatcggc caactctgcc tactgcggct caccagtccg
     2341 gcggagaatc cctacggcag cgcgaaagcc ggctccaagt atcaaggtca gcgcggaccg
     2401 acgccgtcac ggtcgtatca gaacttcatc aagtcgaact agcgggaagt tgaaccgagg
     2461 cactcgcgtt tacaacaacg cgggtagatt tgaaaaccgg cgccggcgag catccgcgcc
     2521 gtcgcgatcc ggccagcgtt tcaccgccac tagcaaatgc ggttggctgt aacagccgcc
     2581 ctggtagagg cgatgaagta actagttgtc ggctcgcgcg gaacgaatgc gcaacggcag
     2641 cttagacagc gtagcaaaca ctttggaggg gtcagtggac atcgtattgg gtgtgtcgat
     2701 gacacctaca acggtccgca tggtgctggt cgagggcgaa aaagccgacg gtgtcac
//
LOCUS       DASYLX010001109         7404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2496913,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001109 DASYLX010000000
VERSION     DASYLX010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496913"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGP27_25950"
     CDS             <1..469
                     /locus_tag="VGP27_25950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7854779.1"
                     /translation="EFGVATGTTLKIIADAVGNDRTVVGFDTFTGLPEAWRTGFPAGE
                     FAQQHLPDVAGAELIVGLFEDELPGFLARNEDQIAFMHVDCDLYSATKAVLDLTTERL
                     APGAVLLFDEFFNFPGWQQHEFRAWTEFLERTGRTFEYLAYTGNSEQVVVRLH"
     gene            complement(466..2340)
                     /locus_tag="VGP27_25955"
     CDS             complement(466..2340)
                     /locus_tag="VGP27_25955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7854780.1"
                     /translation="MTERGETRPALTEHAAPERLVAARGLFTGPGAQVPDDLYARIVD
                     GRAQRERYALHLEKGATVDTNTYFGRFPASYYQRWTTIGEVQLKLAFKAAGPARVLLR
                     ASDAHDNVRTITSTEVDGTGTAVLSAALNEFVDGGALWMECTAVRGPLTITDLQWTVT
                     APPAIRPAAIAICTYNRPDDCAATLAAITNDKGLLAGIDAIYVVDQGNDCVCEKALFK
                     DISAQLGDKLVYLQQPNLGGSGGFTRGMYEVSSITDHANVILMDDDILCEPDAVLRLN
                     AFANLTPTPTIVGAQMLYLKNPRYLLAGAEDPDLVRLRPGRWAANSLHNDDVVKKRQN
                     KRTDATYTGWWTCLIPAEVIAAVGLPLPLFIKWDDIEYALRAGTHGFPTVTLPNAGVW
                     HAEFHWKDYDDWVHYFQIRNALVTAALRGNINAREASRAFAREIAECLGSMRYGLAHL
                     IIRAIEDFLEGPSILEDGGAGAVARVRAERAQYPETKAHPAVSTAELTNAVPPIRPSS
                     KHHPRKDRLDLVRAKRVAYQWLGRTVGGPVMIPAADAHWWHIGIFDYAVVTDASQGGV
                     RILRRDKDKLKTLTKRATKTLRRFRKQAPRLQDRYRETFPDLISRENWARLFESSQNG
                     "
     gene            complement(2471..2914)
                     /locus_tag="VGP27_25960"
     CDS             complement(2471..2914)
                     /locus_tag="VGP27_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7854781.1"
                     /translation="MATVDVEVHTTIERSRPEVAAYSCDPDNVTTWYANIRTVHWESA
                     RPLALGSRFRFTSGFLGRTLEYTYEVVELMPAERFVMRSDSGPFPMETSYAWEDTEDG
                     GTWMTLRNRGEPTAFAGLAAPILATAIRRATARDLVKLKAILEGR"
     gene            complement(2932..4317)
                     /locus_tag="VGP27_25965"
     CDS             complement(2932..4317)
                     /locus_tag="VGP27_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV7854782.1"
                     /translation="MAVSGTSSGPELLIELNRAASEPLHRQLADGLRTAIRTGRLAPH
                     TRMPSTRVLATDLGVSRRLVVDAYSQLAAEGFLLSRRGSGTKVATVETASAPQHNEQA
                     RRYDVDFLPGSPDLGSFPRAAWLRALRLGLADIGSESFGYVEPQGLAAARAAVADYLR
                     RTRGVQADAQHIVLCSGATQAIALLGRALQESDLPLALEDPGFWLHRMILRHNGIEPV
                     AVPIDDDGLDVAALADSGANTVLTTPAHQSPTGVVLSATRRTALVEWARAGHLIIEDD
                     YDAEYRYDRAPVGALQGIAPDRVVYIGSTSKTLAPGLRIGWMVLPPQLIGAIRLSKTL
                     ADTGSSVMDQIAFASLMSTGGYDRHLRQMRRRYLLRRNALLEALARYLPQATVLGAAA
                     GVHLTVRFPDGYPIADLVRRAAAMRVNVEPLAPCYADPATAPPGLLLGYANLTESAID
                     AGVQTLSRAAG"
     gene            4433..4807
                     /locus_tag="VGP27_25970"
     CDS             4433..4807
                     /locus_tag="VGP27_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854783.1"
                     /translation="MTLIVFGVYALGLGLFMLFFPGAFFDTLGTFGVRNDHYIFDNAT
                     FEIPQGLLLLAAVRRPSWRVPALAFATLHWALHSLSHIIDPHHGAGDLFGWLEAGGLV
                     ATTVILAIALRASVNADEARDR"
     gene            4807..5715
                     /locus_tag="VGP27_25975"
     CDS             4807..5715
                     /locus_tag="VGP27_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854784.1"
                     /translation="MRVLVAGATSVPGLPLLRELDARGHEVIGLTRSSDKAPRIEAAG
                     AKPVVADVFDADDIDKAVADIGPEVVVSLLTTLPKLGPKSVKDFEPARKLWGTGASNL
                     VAAAQRAGVRRIVAESVIFAYGYPTSGPVVMDETDPYPGPPPKGGDQMLAALRGMERN
                     VLSSGEHSDTQGIVLRYGCFYGPGVPHDELFVRLAKWCMLPAMTGNGIVSWIHVDDVA
                     TATADALDRGRGGQIYNIVDDRPQSFGDYVRELSSKLHRPRPISISPRLVSLVASYPA
                     TAFGDTWLPLSNAKAKAELGWTPIRR"
     gene            complement(5702..6526)
                     /locus_tag="VGP27_25980"
     CDS             complement(5702..6526)
                     /locus_tag="VGP27_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7854785.1"
                     /translation="MGDTYESLTVDFSDHVAQVTLTGPGKGNAMGPAFWAEMPEVFAT
                     LDADRDVRAIVLTGSGKNFSYGLDLIAMGGSLSGVLADGATARPRADFHATILRMQGA
                     ISAVADCRTPTIASVHGWCIGGGVDLISAVDIRYASADAKFSVREVKLAMVADVGSLA
                     RLPLILNDGHLRELALTGKNIDAARAEKIGLVNDVYDDAEASLAAAHETAAEIAANPP
                     LTISGIKDVLDQQRIARVSESLRYVAVWNSAFLPSKDLGEGMKATFEKRPPNFTGE"
     gene            complement(6545..7003)
                     /locus_tag="VGP27_25985"
     CDS             complement(6545..7003)
                     /locus_tag="VGP27_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854786.1"
                     /translation="MRSHLRVTAAALIFVGLAAGCSRTTEGAVAQTTEPGPPIESPTR
                     TRSAEPGFPSIPGLPNIPGLPQIPGLPMPGNTNVPEVPAPANWKTMTCSEYTKLDEAG
                     QRAVIKAILEDSGQGQTPESQMVAALMATTMCGFLPSAVVKEVVLGGSPP"
     gene            7045..>7404
                     /locus_tag="VGP27_25990"
     CDS             7045..>7404
                     /locus_tag="VGP27_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family protein"
                     /protein_id="HEV7854787.1"
                     /translation="MSADLPPGPNSPPTDELDSAEASLHVLQNVVHPIGDDDRRRQTP
                     CSEFDIEKLTDHLMNSITVIGAAAGAELPERDAGESVERQVVAAARPALDAWHRRGLD
                     GTVKFGDNEAPAQMMAGI"
BASE COUNT         1220 a         2367 c         2528 g         1289 t
ORIGIN      
        1 ggaattcggc gtggcgacgg gaacgacgct gaagatcatc gccgacgccg taggcaacga
       61 ccgcaccgtc gtgggtttcg acacgttcac cggtctgccc gaggcatggc ggaccggctt
      121 tcctgcaggg gaattcgccc agcaacacct accggacgta gcaggtgcag agctaatcgt
      181 cgggttgttc gaggatgagc tgccaggttt cctggcacgc aacgaagatc agatcgcttt
      241 catgcacgtt gactgcgact tgtactcggc aacgaaagca gtgcttgatc tgactaccga
      301 acggcttgcc ccgggtgcgg tcctgctatt cgatgaattc ttcaacttcc ccggctggca
      361 gcagcacgaa tttcgagcct ggacggagtt tctggagcgg accggacgca ccttcgagta
      421 cctggcctac accggcaaca gcgagcaggt cgtcgtccgg ctgcactaac cgttctggct
      481 gctctcgaaa agacgggccc agttctcgcg gctgatgagg tcggggaacg tttcgcggta
      541 gcgatcctga aggcggggcg cctgtttacg gaaccgccgt agcgtcttgg tcgccctttt
      601 ggtgagcgtc ttcagtttgt ctttatcgcg ccgcagaatg cgcacgccgc cctgcgaggc
      661 atcggtgacg accgcgtagt cgaagatccc gatgtgccac cagtgggcat ccgcggccgg
      721 gatcatcacc ggtccgccga cggtgcggcc gagccattga tacgcaacgc gtttcgcacg
      781 gaccagatcc agccgatcct tgcggggatg atgcttcgac gacggccgga tcggcggtac
      841 ggcattcgtc agttcggctg tgctgaccgc ggggtgtgcc ttcgtctcgg ggtactgggc
      901 gcgctcggcg cgaacccgcg ccacggcgcc tgccccgccg tcctcgagaa tcgatgggcc
      961 ctcgagaaag tcctcgatcg cgcgaatgat gagatgtgcc aggccgtatc gcatcgagcc
     1021 gaggcactcc gcgatctccc gcgcgaatgc tctactagct tcgcgcgcgt tgatgttgcc
     1081 gcgcagcgcg gcggtgacca aagcgttgcg gatctggaaa tagtggaccc agtcgtcgta
     1141 gtccttccaa tggaactccg catgccagac gccggcattg ggcagggtga cggtggggaa
     1201 gccgtgcgtg ccggcgcgaa gggcgtactc gatgtcgtcc cacttgatga ataaaggcag
     1261 tggaagacca acggcggcaa tgacttccgc cggaatgagg caggtccacc agcccgtgta
     1321 ggtggcatcc gtccgcttgt tctgacgctt cttgacaacg tcgtcgttgt gcaacgagtt
     1381 ggctgcccac cgtcccggtc gcagccttac gagatcggga tcttccgcac cggccaggag
     1441 gtaacgcgga ttcttcaggt acagcatctg cgcgcccacg atggtcggag tcggcgtgag
     1501 attggcgaac gcgttcagcc gcaacaccgc atcgggctcg cagaggatgt cgtcatccat
     1561 caggatgacg ttggcatgat cggtgatgct cgacacctcg tacatgccgc gggtaaagcc
     1621 accgcttcca ccaaggttcg gttgctgcag gtagaccaac ttatcgccga gttgcgcgga
     1681 gatgtctttg aacagcgcct tctcgcaaac acagtcgttg ccttgatcga cgacgtagat
     1741 ggcatcgatg ccggcaagca gccctttgtc gttggtgatg gcggcgagtg tggcagcgca
     1801 gtcgtcgggc cggttgtatg tgcagatggc gatcgccgcg ggccggatgg caggcggagc
     1861 ggtcacggtc cactggagat cggtgatcgt caacgggccc cgaacggcgg tgcattccat
     1921 ccatagcgcg ccgccgtcga cgaattcgtt gagtgcagcg gagaggactg cggtgccggt
     1981 gccgtcgacc tcggtgctcg tgatcgttcg gacgttgtcg tgggcgtcgg acgctcgcag
     2041 gagcacccgc gcgggccccg ccgccttgaa ggccagcttg agctgtacct caccgatcgt
     2101 ggtccaccgc tggtagtagc tcgcggggaa gcgaccgaag taggtattgg tgtccacggt
     2161 ggcacccttt tccaggtgca gggcgtagcg ctcgcgctga gcccggccgt cgacgatgcg
     2221 cgcatacaga tcgtcgggca cctgcgcgcc gggcccggtg aacagaccgc gtgccgctac
     2281 cagccgttct ggcgcggcgt gctcggtgag cgccggacgg gtttccccgc gctcggtcat
     2341 gctgctccta gtgtcataaa cgtggggcgc ttaccagctt tccccaaggt ccccgaggga
     2401 gcgtaacaat ctgacgtgca gagacaagtt gacggcgcgc agaccagcgg ttaaatcttg
     2461 aacaggcagg tcagcggccc tcgaggatcg ccttgagttt aaccagatct ctggcggtgg
     2521 cgcgcctgat ggccgtcgcc aggatcggcg ccgccagccc ggcgaacgcg gtcggctccc
     2581 cacggttgcg aagcgtcatc caggtcccgc cgtcctcggt gtcctcccac gcatagctgg
     2641 tctccattgg aaatgggccg ctgtcggacc gcatcacgaa tcgttccgcc ggcatcagtt
     2701 cgaccacctc gtaggtgtac tccagcgttc ggccgagaaa gccgctggtg aaccggaaac
     2761 gagagcccag cgctaacggc ctcgccgatt cccaatgcac cgttctgata ttggcgtacc
     2821 aggtcgtgac gttgtccggg tcgcagctgt acgccgcgac ctcggggcgc gatcgctcga
     2881 tggtggtgtg gacctcgaca tcgacggttg ccatccccac agtgtcgcgg ctcagccggc
     2941 cgcccgcgac agcgtctgaa ccccggcatc gatcgctgac tcggtgaggt tggcgtagcc
     3001 gagcagtagc ccgggcggtg ccgtcgcggg gtccgcatag cagggcgcca gtggctccac
     3061 gttcacccgc atggccgccg cgcgacggac caggtcggcg atcgggtacc cgtcgggaaa
     3121 gcggacggtg aggtggaccc cggcggcggc gccgagcacg gtcgcctgcg ggaggtaacg
     3181 ggccagggcc tccaggagcg cattgcgacg aagcagatat cgacggcgca tctgccgcag
     3241 gtggcggtcg tagccgcccg tgctcatcag cgaggcgaac gcgatctggt ccatcaccga
     3301 gcttccggtg tccgcgaggg tcttcgacag cctgatcgcg ccgatgagct gcggaggcaa
     3361 caccatccat ccgatgcgca ggccgggcgc gagtgtcttg ctggtcgagc cgatgtagac
     3421 cacccgatcc ggtgcgatcc cctgcagggc gccgacgggt gcccggtcat agcggtactc
     3481 ggcgtcgtaa tcgtcttcga tgatcagatg accggcgcgc gcccattcca cgagggcggt
     3541 gcgacgagtc gccgagagca cgacaccggt cggcgactgg tgcgcggggg tggtcagcac
     3601 ggtattcgcg ccgctatcgg ccagtgctgc gacgtccaga ccgtcatcgt cgatcggcac
     3661 cgcgacgggc tcgatgccgt tatgccgcag gatcattcgg tgcagccaga atccaggatc
     3721 ctccaaggcc agcggcaaat cgctctcctg aagcgcgcgc ccgagcagcg cgatcgcctg
     3781 ggtcgccccg gaacacagca cgatgtgctg tgcgtcggcc tgcacacccc tggtgcgccg
     3841 gaggtagtcg gcgacggcgg cgcgggccgc ggccaaacct tgcggttcga cgtacccgaa
     3901 cgactccgag ccgatgtcgg cgagaccgag ccgcagcgcg cgcagccacg cggcgcgggg
     3961 aaagctgccg agatccggag agccgggcag gaagtcgacg tcgtagcgcc gcgcctgttc
     4021 gttgtgttgc ggcgcgctgg cggtctcgac ggtcgcgacc ttggtgcctg aaccgcgcct
     4081 gctcaaaagg aagccttcgg cggccagttg gctgtaggcg tcgacgacga gtcgtcgaga
     4141 cacgccgagg tccgtcgcga gcacccgggt ggaaggcatc cgggtgtgcg gtgcgagccg
     4201 gccggtgcga atcgcggtgc gtagcccgtc ggccagctgg cggtgcagcg gttcgctggc
     4261 ggcacggttg agctcgatca gtagctctgg accagaactg gtacccgaaa ccgccatgaa
     4321 agtggtgcct atcttgaggc cattggcgaa ctaccgtgaa cgtatgtcaa cgagagctac
     4381 ccgcacaaga gccgggttcg taccgcgcac gcgaggagcg aagatcatca cagtgacact
     4441 gatcgtcttc ggcgtatacg cgctgggcct cggcctgttc atgctgttct tccccggcgc
     4501 gttcttcgac accctgggga cgttcggggt tcgcaacgat cactacatct tcgacaacgc
     4561 gacgttcgag attccgcagg gtctgcttct gctcgccgcc gtgcggcgac cgtcgtggcg
     4621 ggtgccggcc cttgcgttcg ccacgctgca ctgggcgctg cactcactca gccacatcat
     4681 cgatccgcac cacggcgcgg gcgatctgtt cggttggctg gaggccgggg gtctggtcgc
     4741 caccacggtg attctcgcga tcgctctgcg cgccagcgtg aatgccgacg aggcgcgtga
     4801 tcgctgatgc gcgtcctggt tgccggggcc accagcgtcc ctgggcttcc gctgttgcgc
     4861 gagctcgatg cccgcggtca tgaggtcata gggctgacgc gatcgtcgga caaggcgccc
     4921 cggatcgagg cggcgggtgc caaaccggtg gtggccgacg tattcgacgc cgacgacatc
     4981 gacaaggcgg tcgccgacat cgggcccgag gtggtggtct cattgctcac cacgctgccg
     5041 aagctgggac cgaagtcggt caaggacttc gaaccggctc gaaagctctg gggcacaggg
     5101 gcttccaacc tcgttgccgc cgcacaacgg gcgggtgtgc ggcgtatcgt cgccgagtcg
     5161 gtgatcttcg cctacggcta tccgacgtca ggacctgtgg tgatggacga gacggacccc
     5221 tatcccggtc caccaccgaa gggtggcgac cagatgctgg cggcgctgcg cggcatggaa
     5281 cgcaacgtcc tgtcatcggg tgagcacagc gacacccagg gaatcgtgtt gcggtacggg
     5341 tgtttctacg ggccgggggt gccgcatgac gaattgttcg tgcggctggc gaaatggtgc
     5401 atgctgcccg cgatgaccgg caacggaatc gtgtcatgga tccacgtcga cgacgtcgcc
     5461 acggcgaccg cggacgccct cgaccgcggc cgcggcggcc agatctacaa catcgtcgac
     5521 gaccggccgc agtcgttcgg cgactacgtt cgcgagctct cgtcgaaact ccatcgaccg
     5581 cggcccatat cgatctcgcc ccggctggta agcctggtcg cctcgtatcc tgcgacggcc
     5641 ttcggcgaca cctggctgcc gctgtcgaac gcgaaggcca aggccgaact cggctggacg
     5701 cctattcgcc ggtgaagttc ggcggccgct tctcgaaggt cgccttcatg ccctcgccga
     5761 ggtccttcga cggcaggaac gccgagttcc acaccgccac gtagcgcagg ctctccgaca
     5821 cccgcgcgat gcgctgctga tccagcacgt ccttgatgcc gctgatggtc agcggtgggt
     5881 tggcggcgat ctcggcggcg gtttcgtgtg cggcggccag cgaggcctcc gcatcgtcgt
     5941 agacgtcgtt gaccaggccg atcttctcgg cccgcgctgc gtcgatgttc ttgcccgtca
     6001 acgcaagctc gcgcaggtga ccgtcgttga ggatgagcgg gagccgggcc aggctgccga
     6061 cgtcggcgac catggccagc ttcacctcgc gcaccgagaa cttcgcgtcg gcgctcgcgt
     6121 agcggatgtc gacggcggag atcaggtcga cgccgccgcc gatgcaccag ccgtgcactg
     6181 acgcgatggt cggcgtgcgg cagtcggcga cggcgctgat cgcgccctgc atgcgcagga
     6241 tcgtcgcgtg gaagtcggcg cgcgggcggg ccgtcgcacc gtcggcgagc acgccggaca
     6301 gcgaacctcc catcgcgatc aggtccaggc cgtagctgaa gttcttgccg gagccggtga
     6361 gcacgattgc gcggacgtcg cggtcggcgt cgagcgtggc gaacacctcg ggcatctcgg
     6421 cccagaacgc cgggcccatg gcgttgccct tgccggggcc ggtcagtgtc acctgcgcga
     6481 cgtggtcgga gaagtcgacg gtgagagatt cgtacgtgtc gcccatgacg gagaggctag
     6541 ccactcacgg cggcgagccg ccgaggacca cctctttgac gaccgcgctg ggcagaaagc
     6601 cacacatggt cgtcgccatc agtgccgcga ccatttgaga ctcgggggtc tgcccttgac
     6661 cgctgtcctc caggatggcc ttgatgacgg cgcgctgacc ggcctcgtcg agcttggtgt
     6721 actcgctgca cgtcatggtc ttccagttcg ccggtgcggg aacctcgggc acattggtgt
     6781 tccccggcat cggcaggccc ggtatctgcg gaaggcccgg aatgttgggc aggccgggga
     6841 tgctcggaaa gccgggttcg gccgaccgag tgcgggtggg cgactcgatc ggaggccccg
     6901 gctcggtggt ctgcgcgacc gcgccctctg tggtgcgtga gcacccggcg gccaggccga
     6961 cgaagatcag cgccgcggcc gtcacccgca gatgggatct catctgcgca acgataggcg
     7021 cacgcgcgac gtagcgtggg cttcatgtcc gccgacttgc ctcccggtcc caattcgcca
     7081 ccgacagatg aactcgacag cgcagaagcc agccttcacg tgctgcagaa cgtggtgcat
     7141 ccgatcggcg acgacgaccg gcgcaggcag acaccgtgca gcgaattcga catcgagaag
     7201 ctcacggatc acctgatgaa ctcgatcacc gtcatcggtg ccgccgcagg cgcggagtta
     7261 ccggagcgcg acgcgggcga atcggtggag cgtcaggtcg tcgcggccgc caggcctgcg
     7321 ctggatgcct ggcaccgccg cgggctggac ggcaccgtga agttcggcga caacgaggca
     7381 cccgcgcaga tgatggccgg catc
//
LOCUS       DASYLX010001110         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_851774,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001110 DASYLX010000000
VERSION     DASYLX010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851774"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VGP27_25995"
     CDS             complement(<1..939)
                     /locus_tag="VGP27_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV7854788.1"
                     /translation="MTAPAAGPDQPGESMRGSVHICVTAIVAGVLIGLVGGAFRWCLQ
                     EADELRIDLVEWAHQLPGPGWLIPMAAAAAGATLAALIVRWEPLAAGSGIQHVEAVFL
                     NEARPPLIRLVPAKFIGGVLSMGSGLVLGREGPTVHMGAAIGAEAARRARLPDSEIRM
                     MQTALGGAGLAVAFNAPIGGTLFTLEEVTKSFRVKTVLATLFSAAAAVACSRLILGNH
                     ADFRVEPVGAPALAWLPVFVVFGLLTGCLGAAYNKLVLWFLDHVAAIRRVPALAKAAI
                     IGAIVGFAMFVYPLAVGGGEDLTQRILGGQHIAVSVV"
     gene            993..2972
                     /locus_tag="VGP27_26000"
     CDS             993..2972
                     /locus_tag="VGP27_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV7854789.1"
                     /translation="MVGHGAHHDHVAQFRRLFWIMLVLAVPTVALSGMFSAILGYTVP
                     ELPAARWVSPVLGTVMYFWGGRPFLTGAASEIRSRAPGMMLLIGLAITVAFVSSWGAS
                     LGVLDHQLDFWWELALLIVIMLLGHWIEMRSLAQTSSALESLAALLPDEAERVEEDEI
                     VTVAPSQLRRNDVVVVRPGSSVPADGEIVDGAADMNESMVTGESRPVRRGVGERVVAG
                     TVATDSGLRVRVTAVGDDTALAGIQRLVTEAQNSSSRAQRLADTAAGWLFWFALGAAV
                     ITAVVWGFIGRPEEAVIRTITVLVIACPHALGLAIPLVVSIATERAARAGVLVKDRLA
                     LETMRTVGAVLFDKTGTLTKGEPSVTAVATADGHSELDVLAWAAAAEAGSEHPLATAI
                     VAAARKRNVKIRPASGFSSSPAVGVTATVDGRKVRVGGPRLLREAGQPELPDAWTWHD
                     EGAIVLHVLVDGSVVGALKLADEVRPESRQAVDALHRLGIEVVMVTGDAEPVAMAVAR
                     ELGIDRVFAGVRPEEKAAKVAELQGEGRRVAMVGDGVNDAPALAQADVGIAIGAGTDV
                     AIASAGVILASSDPRSVLSVIRLSKATYRKMQQNLWWAAGYNLVAVPLAAGVLAPIGF
                     VMPMSVGALLMSASTVVVALNAQLLRRLDLRPDVQ"
     gene            complement(2966..3451)
                     /locus_tag="VGP27_26005"
     CDS             complement(2966..3451)
                     /locus_tag="VGP27_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854790.1"
                     /translation="MPLSDLVRNVGDVLSRADTLFGTADPSTTAAAADRLTDATDTIR
                     AGQEQAASMTGDAITDYTDFAQSTRDALDHLSGVESDLGHHLQEAAEAVTAARSASQS
                     ALHVVSSASDAVGHMTDSPAADLAVIRALRAQVADELELIRTHQAVAEQLGAQLRGLG
                     Y"
BASE COUNT          549 a         1178 c         1275 g          588 t
ORIGIN      
        1 gacgaccgag acagcgatgt gctgtccgcc gaggatgcgt tgcgtcaggt cctcgccgcc
       61 gcccaccgcg agcggataca cgaacatggc gaacccgacg atggcaccga tgatggctgc
      121 cttcgccagc gccgggactc ggcggatcgc ggcgacgtgg tcaagaaacc acagcaccaa
      181 tttgttgtaa gcggcaccaa gacatccggt cagcagtccg aaaacgacga aaacaggcag
      241 ccacgcgaga gcgggtgcgc cgacgggctc gacccggaag tcggcgtggt ttcccaggat
      301 cagccgtgag caggccacag ctgcggcagc cgaaaacagc gttgccagga ctgttttcac
      361 gcggaacgac ttcgtcacct cttcgagggt gaagagggtg ccgccgatcg gcgcgttgaa
      421 cgcgaccgcc aggccggcgc cgccgagcgc cgtttgcatc atcctgatct cggaatcggg
      481 cagccgggcc cgccgggccg cttcggctcc gatcgcggca cccatatgca ccgtcggccc
      541 ttcgcggccc agcacgagtc ctgaccccat gctcagcaca ccaccgatga acttcgcagg
      601 aaccaatcgg atgagcggcg gccgggcctc gttgaggaac accgcttcga cgtgttggat
      661 accgcttccg gcggccagcg gttcccatcg cacgatcagc gcggccagcg tcgcgcctgc
      721 cgcggcggcg gccatgggga tcagccaccc gggcccgggc agctggtgcg cccattcgac
      781 aaggtcgatg cgcagttcat cggcttcttg aagacaccag cgaaaagctc cgccgactaa
      841 accgatgagc acgcccgcga cgatcgccgt gacacagatg tgtaccgaac cccgcatcga
      901 ttcgccgggc tgatcgggac ctgccgccgg tgccgtcatc ggcgacgaac tttacctgcc
      961 tggatacccc catggggtat agaatgctgc gcatggtcgg ccacggcgca catcacgacc
     1021 acgtcgcgca gttccgcagg ttgttctgga tcatgctcgt gctggccgtg ccgacggtgg
     1081 cgctgtcggg catgttttcg gcgattctcg gctacaccgt gcccgagttg cctgctgcgc
     1141 gttgggtctc gccggtgctc ggtaccgtca tgtacttctg gggcggccgg ccgtttctga
     1201 ccggcgcggc cagcgagatc cgttctcgcg caccgggaat gatgctgctg atagggctgg
     1261 ccatcacggt ggcgttcgtc tcgtcgtggg gcgccagtct cggcgtgctc gaccaccagc
     1321 tcgatttctg gtgggagctg gcactgctga tcgtcatcat gcttctcggg cactggatcg
     1381 agatgcggtc gctggcccag accagttcgg cgctggaatc ccttgccgca ctgctgccgg
     1441 acgaggccga gcgggtcgag gaggacgaga tcgtgacggt cgctccgtcg cagctacggc
     1501 gcaatgatgt cgtggtcgtg cgccccggca gcagcgtgcc cgccgacggc gagatcgtcg
     1561 acggcgcagc cgacatgaac gagtcgatgg tcaccggtga gtcccgaccg gtgcgccgcg
     1621 gcgtgggcga acgcgtcgtc gccggcaccg tcgcgaccga ttccggcctg cgcgtgcggg
     1681 tcaccgccgt cggcgacgat accgcgctcg cgggcatcca gcgactggtg accgaggcgc
     1741 agaactcctc gtcgcgggcg cagcggctgg ccgacaccgc ggccggctgg ctgttctggt
     1801 tcgcgctggg cgcggccgtc atcaccgcgg tggtgtgggg cttcatcggc aggccggagg
     1861 aagcggtcat caggaccatc accgtgctgg tgatcgcctg cccgcacgcg ctgggcctgg
     1921 cgataccgct ggtggtgtcg atcgccaccg agcgggcggc ccgcgccggt gtgctggtga
     1981 aggaccggct ggcgctggag accatgcgga ctgtcggcgc ggtgctgttc gacaagaccg
     2041 gcaccctgac caagggcgag cccagtgtca ccgccgtcgc gacggcggac ggccactcag
     2101 aactcgacgt gctcgcatgg gccgcggccg ccgaagcggg ctccgaacat ccgctcgcca
     2161 ccgccatcgt cgcggcggcg cggaagcgca acgtcaagat ccgtccagcg tcgggctttt
     2221 cgtcgtcgcc cgccgtgggg gtgacggcga cggtcgacgg ccggaaggtc cgcgtcggtg
     2281 ggccgcggct attgcgggag gcgggtcagc cggagttgcc cgatgcatgg acgtggcacg
     2341 acgaaggcgc gatcgtgttg catgttctgg tcgacggatc cgtggtgggc gcgctgaagc
     2401 tggccgacga agtgcgaccg gagtcgcgtc aggccgtcga cgccctgcac cgccttggta
     2461 tcgaggtcgt gatggtcacc ggcgacgccg agccggtagc catggcggtc gcccgcgagc
     2521 tcggcatcga tcgggtgttc gcgggtgtgc ggcccgagga aaaggcggcc aaggtggccg
     2581 agctgcaggg tgaagggcgc agagtcgcga tggtcggcga cggcgtcaac gacgcgcctg
     2641 cgctggccca ggccgacgtt ggcatcgcca tcggcgcggg gaccgacgtc gcgatcgcat
     2701 cggccggtgt gatcctcgcg agctcggatc cgcgttccgt gctttcggtg atcaggctgt
     2761 ccaaggcgac ctatcgcaag atgcagcaaa acctttggtg ggcagcggga tacaacctcg
     2821 tcgccgttcc gctggccgcc ggtgtgctgg cgccgatcgg cttcgtgatg ccgatgtcgg
     2881 ttggtgcact gttgatgtcc gcgtcgacgg tggtggtcgc gctcaatgcg cagctgctcc
     2941 gacgtttgga cttgcgcccc gacgttcagt agccgagccc gcgcaactgc gcgccgagct
     3001 gctcggcgac cgcctggtgg gtgcggatca actcgagctc gtcagcgacc tgtgcgcgca
     3061 gggcacggat aacggccaaa tcggcggcgg gtgaatccgt catgtgcccg accgcatctg
     3121 atgccgacga caccacgtga agcgccgact gcgaggccga gcgcgccgcc gtcaccgcct
     3181 ccgcggcctc ctgcaggtgg tggccgaggt ccgactcgac gcccgacagg tggtcgagcg
     3241 cgtcgcgggt actctgcgcg aaatcggtgt agtcggtgat cgcgtcaccc gtcatcgacg
     3301 cggcctgttc ctgccctgct cggatggtgt ccgtcgcatc ggtcagacgg tcggcagccg
     3361 cggcggtcgt gctggggtcg gcggtgccga acagcgtgtc ggcgcgcgac aacacatcgc
     3421 cgacgttacg gaccagatcg gacagcggca cggaacccag tatcagctat cgagtgacgc
     3481 gatgaggtca ttcaggatgg cggtgtctgc gtcggtcgca ggctcgccgg cctcgaccgc
     3541 gtgcacgagc tcgaccggta ggcctgctgc gttagcagtc tcggcggccg
//
LOCUS       DASYLX010001111         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1947593,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001111 DASYLX010000000
VERSION     DASYLX010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947593"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1591
                     /locus_tag="VGP27_26010"
     CDS             101..1591
                     /locus_tag="VGP27_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEV7854791.1"
                     /translation="MTDRDDFLREHLSPPSDSGDEPVGRPPEPPRPPAQDDPGSRNGL
                     EDGPRPAGSDSVRRPPEEPGQPTGPDARPRWTTEASRTHAGPPRIAPGGFRQAPPPMP
                     PRPTEPGAPANPDGRPRESTEAESTRVMPPPGARPAPPGWPQEPPRERTARPASQDWS
                     SQPSSSPNWAAAPAAAPPAAGYSYVDSIRTSELVPSRKTPPRKGWRRFVYRGTFGLVN
                     LGPSPDEQRMADLDNKIRSLLRGHYKIGVFGKGGVGKTTVSASVGSIFAELRQDDRVV
                     AIDADTAFGKLGSRIDPNAAGSYWELAADEHLDTFADVRSRVGNNASGLFVLAGESST
                     ARRRVLDPAIYREATARLDRHFTISIVDCGSTMDSAIAQEVLRDLDALIVVSSPWVDG
                     ASAAGQTMEWLANRGYTGLLHRTVVVLNDSDGHADKRTRSILVQQFASRGQVVVEVPF
                     DSHLRPGGVIDVGAEMSSLTRRRFIEITAALAEHFASTTDGPRDRR"
     gene            1588..>2643
                     /gene="eccD"
                     /locus_tag="VGP27_26015"
     CDS             1588..>2643
                     /gene="eccD"
                     /locus_tag="VGP27_26015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEV7854792.1"
                     /translation="MTTVREPAPTSVGRTATPVEVPPSCRVSILAGDSHQIDLVLPSA
                     APLNTLVDPTVIAINKVLRGRGAAELPPAAYEFARAAGLSALASDVSLAGHNIVDGDV
                     LALVPAGRAERYGPVVENVSTALAQYASDHFVRVTAQTALAVAVLIIGGALGVAGMLL
                     WRLRWAHESALLVPILYAAVTIGLLGAVAIGARVGAGRTVTDGAAWAALVTAVLAAAT
                     IPPGASPGAPHAFLAAATALAGVWLLVRFTGRFWTAAAAIVTAGFGAAAVALVRMYWD
                     VPGPRIAVAVLVAILVAVSAAPTIALRLANVPRQTFGSITGRDIFARAPGQPEDTVSP
                     VEDGPPSDVTLTGEQVAA"
BASE COUNT          373 a          956 c          914 g          400 t
ORIGIN      
        1 gaagctgctc cgctcaccca ggcgcagaag ccgcaatgac cgcccgcagt caacgacatt
       61 caccggagga gaccatggac aggcagtcag gattggcgcc gtgaccgacc gcgacgactt
      121 tctccgggaa cacctttcgc ccccatcgga ttccggcgac gagccggtgg gccgaccgcc
      181 cgagccgccg cggcccccgg cgcaggacga cccgggttcg cgcaatggcc tggaggacgg
      241 tccgcgcccg gccggatccg actccgttcg caggccaccg gaggagcctg gccagccgac
      301 gggtcccgat gccagacccc gttggacgac cgaagcaagc cggacccacg ccggaccccc
      361 acgcatcgcg ccaggcggct tccgccaggc tcccccgccg atgccgccgc gaccgaccga
      421 accgggcgcg cccgcaaacc cggacggcag gcccagggag tcgacggagg ccgagtcaac
      481 ccgggtgatg ccgccgcccg gcgcccgacc ggcgccgccg ggctggccgc aggagccgcc
      541 gcgagagcgg accgcgcgtc ctgcgagcca ggactggtcg tcgcagccgt cgtcgtcgcc
      601 caactgggcc gccgctccgg cagccgcgcc accggcggcg ggctactcct atgtcgacag
      661 catccgcacc agcgagctgg tgccgtcgag gaagaccccg ccgcgaaaag gctggcggcg
      721 tttcgtctat cgcggcacgt tcggactcgt caacctcggt ccgtcgcccg acgagcagcg
      781 gatggccgat ctggataaca agatccgttc gctgctgcgc ggccactaca agatcggggt
      841 gttcggcaag ggcggtgtcg gtaagacgac cgtctcggcc agcgtcggct caatcttcgc
      901 ggagctgcgc caggacgacc gggtggtggc catcgacgcc gacaccgcgt tcggcaagct
      961 cggcagccgc atcgacccga acgccgcggg ttcgtactgg gagctggcgg ccgacgaaca
     1021 cctcgacacc ttcgccgacg ttcgcagcag ggtgggcaac aacgcctcgg ggctgttcgt
     1081 gctcgcaggt gaatccagca cggcccggcg ccgggtgctc gaccccgcga tctaccggga
     1141 ggcgacggct cggctggatc ggcacttcac gatctcgatt gtggactgcg gctcgacgat
     1201 ggactccgcg atcgcgcagg aggtgctgcg cgatctcgac gcgttgatcg tggtgtcctc
     1261 accgtgggtc gacggcgctt cggcggccgg ccagacaatg gagtggctgg ccaatcgcgg
     1321 ctacaccggt ctgctgcatc gcaccgtggt ggtcttgaac gactctgacg gccatgccga
     1381 caagcgcacc cgttcgatcc tcgtgcagca gttcgccagt cgcggccagg tcgtcgtcga
     1441 ggttccgttc gactcacatc tgcgccccgg tggcgtcatc gacgtcggcg ccgagatgtc
     1501 gtcgttgacc cggcggcggt tcatcgagat cacggccgca ctcgccgagc acttcgcgtc
     1561 caccaccgac ggaccgcggg accgccggtg acgaccgtcc gcgaaccggc gcccacatcg
     1621 gtcggccgca ccgcgacccc ggtcgaggtg ccaccgtcgt gtcgggtgtc gatccttgca
     1681 ggcgactctc atcagatcga tctcgtcttg ccgtcggctg cgccgctcaa caccctggtc
     1741 gatccgacgg tcatcgcgat caacaaggtg ctgcgcggtc gtggcgctgc cgaattaccg
     1801 cccgctgcct acgaattcgc gcgtgccgcg ggcttgtcgg ccttggcgtc ggacgtctct
     1861 cttgccggcc acaacatcgt cgacggcgac gtcctagcgc tggtgcccgc aggcagggcc
     1921 gaacgctacg ggccggtggt ggagaacgtt tcgacggcgc tggcgcaata cgcctccgat
     1981 cacttcgtgc gggtcaccgc gcagaccgcg ctggcggtcg ccgtgctgat catcggcggt
     2041 gcgctcggcg tggcaggcat gctgctgtgg cggttgcgct gggcgcacga aagcgcattg
     2101 ctggtgccca tcctgtacgc cgccgtgacg atcgggctgc tcggcgcggt ggcgatcggt
     2161 gcgcgggtgg gcgcgggccg gaccgtcacc gacggcgcgg cttgggctgc gctggtcacg
     2221 gcggtgctgg ccgcggcgac cattccgcct ggagcttccc ccggcgcgcc gcacgccttt
     2281 ctggccgcgg ccaccgcgct ggcgggcgtg tggctgttgg tccgcttcac cggccgattc
     2341 tggacggccg ccgctgcgat cgtcacggcg ggtttcggcg cggccgcggt cgcgctcgtg
     2401 cggatgtact gggacgtgcc gggaccccgg atcgccgttg ccgtactggt ggcaattctg
     2461 gtagcggtgt ctgcggcgcc caccatcgcg ctacggctgg cgaacgttcc gcgacagacc
     2521 ttcggctcga tcacgggccg cgacatcttc gcccgcgctc ccggccaacc cgaagacacg
     2581 gtgtcacccg tcgaggacgg tccgccctcc gatgtcaccc tcaccggcga acaggtggcc
     2641 gcc
//
LOCUS       DASYLX010001112         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_743350,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001112 DASYLX010000000
VERSION     DASYLX010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743350"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGP27_26020"
     CDS             <1..344
                     /locus_tag="VGP27_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854793.1"
                     /translation="FQLPRCTTPKHLQDAAIQNGLAVEEADTSDAGSLLAGRDVPKDA
                     SLRRRGGLHSYERWSGSGRTNGVARCIAFCSTTRVAAAASPVFETIASRNDIESIAPE
                     PDMIAESTESR"
     gene            complement(649..1041)
                     /locus_tag="VGP27_26025"
     CDS             complement(649..1041)
                     /locus_tag="VGP27_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854794.1"
                     /translation="MPSSGDPTKDACDLFNKASNYAAINYEDFADYSAGSGNYVNYQD
                     QTVENANTAGRTALRQAAAAALSASGVPGAAPEVTAPMRNWSLSAAKLVLIMGVRGGG
                     DTLNSTANNLNRQAKEAQMACATAETQS"
     gene            1665..2333
                     /locus_tag="VGP27_26030"
     CDS             1665..2333
                     /locus_tag="VGP27_26030"
                     /inference="COORDINATES: protein motif:HMM:NF020765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEV7854795.1"
                     /translation="MLVPIGTAPLALADEPAAPPPAGDAAPPGAPGGPGPAGPVPSAP
                     PGVLKTPDGYTLTVQGATESMEPVAALTGAPTSREYLVDGTFTGSVTGSGGPKLAGGS
                     LEAGYQIGCGIAQDDIESVISAGITPGVGIPLVTGSIFPITLQPSLSYQMKIDLKPGT
                     VNIVPVDKKTFKGTSPKINITGLRVKFDQCAGSSFIRSYATLTSSTDDTDDVVTYVGV
                     TKMV"
     gene            2751..3428
                     /locus_tag="VGP27_26035"
     CDS             2751..3428
                     /locus_tag="VGP27_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEV7854796.1"
                     /translation="MLNRFATVAAVCMLVPVWTAAVALADDDPPPAVPAGDVGPPPDT
                     GVVPAGPPGIAHASEGRTLTVVAKDETQLPIAPLTTSLASRDWLVGATFTGSGMGSAK
                     GGSLEVGYQIGCAIEMDKVKLNGSIGIGSNAGSFTGNALNFPTALTFPIQGQIEVEPR
                     PGTVTNVVVDKKSYKGNSTRVTVKDIHIKVDNCVGASSLRSYAILTSSNGDTDDVVAY
                     YGVTKVF"
     gene            3435..>3714
                     /locus_tag="VGP27_26040"
     CDS             3435..>3714
                     /locus_tag="VGP27_26040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854797.1"
                     /translation="MIRPHVISSHRVTVQKHLTAGLAAGMLAVGAAVASAATAAAEEP
                     PAPAPAPADPAAPAAAAPSGFLPPGGGVQNFLPEGGLAPGSGYDFLLGQ"
BASE COUNT          677 a         1174 c         1159 g          704 t
ORIGIN      
        1 cgtttcagct tccgagatgc acgacgccta aacacctgca ggacgccgcg atccagaacg
       61 gattagcagt tgaagaagca gacaccagcg acgccggttc ccttctcgcc gggcgtgacg
      121 taccgaagga tgcttctttg aggcgacggg gtgggcttca cagttatgag cgatggtccg
      181 gttctggtcg cactaacggt gtcgcccgat gcattgcctt ctgttcgacc acacgcgtcg
      241 cggccgcggc ctcgccggtt ttcgagacca tcgcgtctcg taatgatatc gaatcgattg
      301 cgccagagcc cgatatgatc gcggagtcga ctgaatcccg ctgaaattta tggccgctcg
      361 agcggagtac acgccggagg tggggccaat cgattcatga acgtagggac cgtgcggtgt
      421 gaaactttca acgactgcga cttgcgccac gtcaaacccc gatgcggccg cgtctgggtc
      481 ccgatgttgg cccgtgacaa tccaatgtca gcggcaacgc gagcgcgttc tccaccgcac
      541 cggtattcga cggcctcagc tgccgcagtt cgctgtcgac ttcgctcgca gccaggatga
      601 cgccggtggc cgtggccggc gtccccggca gtttgagtct gcctggagtc agctctgcgt
      661 ttccgctgtt gcgcaagcca tctgcgcttc cttggcctgc ctattgagat tgttggccgt
      721 cgaattgaga gtgtcaccgc cgccgcggac acccataatc aagacgagct tcgcggcact
      781 caaggaccag ttccgcatgg gtgccgtgac ctcaggcgcc gcacccggca cacctgacgc
      841 cgacagcgcg gccgcagccg cctgcctcag ggccgttctc cctgcggtat tcgcgttctc
      901 aacagtctga tcttgataat tcacatagtt tccgctgccg gcgctgtagt ccgcaaaatc
      961 ctcgtagttg atcgcggcgt agttcgacgc tttgttgaac agatcgcaag cgtctttcgt
     1021 cgggtcaccg gaagagggca tttgggcagt ctgggggtcc acaccctgag gagtaccaga
     1081 cggtccgatg ggtgctccgg gaacagcggc cgattccggg attgcaccgg gcgcatccgg
     1141 tggcgcctcc cccgggacgg gagcgggcgg ttgaagctga tcagcgcccg cggggtcggc
     1201 ggacgcggca gggatgagag ccaatccgat gccgaatgcc atcagggtgg tagtcaccat
     1261 ctgccgacag aagtgaaatg acctcatcgt ttccttccaa actgtcaatc tgtcgctgcc
     1321 cgtcccggcc gccgggtcta tgcaaagcca tgcgaataac aaggccgcct ccgcgatcga
     1381 tggtgggtcg ggcacctttc tcatctgtga acaaatgcca accagccggt tccggcgagt
     1441 aatccattgc gtgtctccga gtttcgtcgc cgcagccgcg cggtacttcg aaggcgtgag
     1501 acgtccggta gatgggaatc gggccctagg cgttcgcgct gtgttcacgg ttcggacact
     1561 cgctccctca aagattcctc aggacagata ccgtggggaa ttcggacgat gagaggcgcc
     1621 aaaacgacat gattaatcgg tttgccacgc tggctgcagc ttgcatgctg gtcccgatcg
     1681 ggacagctcc actggcacta gcggatgagc cggccgcacc gccgcctgcc ggcgacgccg
     1741 ctcctcccgg ggctccgggc ggccctggac ccgccgggcc tgtgccatcc gctcctcccg
     1801 gcgtcctgaa gacgccagac ggttacaccc tcacagtgca gggcgccaca gaatcgatgg
     1861 agccggtggc agcgctgacg ggtgcgccga cctcacgcga gtacctggtg gacggcacgt
     1921 tcaccgggtc ggtgaccggt tcaggtggtc cgaagctcgc aggcggatcg ctcgaggccg
     1981 gctaccagat cggctgcggc atcgcccagg acgacattga aagcgtcatc tcggcgggca
     2041 tcaccccagg cgtcggcata ccactagtca ccggcagtat cttccccatt acgctgcagc
     2101 cctcgctgtc gtatcagatg aagatcgacc tcaagcctgg cacggtcaac atcgttccgg
     2161 tcgacaaaaa gacgttcaaa ggcaccagcc cgaagatcaa tatcactggt ctacgtgtca
     2221 agttcgacca gtgcgcgggg tcgtcgttca tccggtcgta cgcgacactg accagttcga
     2281 ccgacgacac cgacgatgtg gtgacctacg ttggcgtcac gaaaatggtg tgacgcgacc
     2341 agatttcgtc cgtagacgga tctcggcgta gctgacccga aacttctccc atcgcatgcc
     2401 cctcacagaa gtggggggca tgcgtcgttg gagccgagcg ggtgacgtag cgccgattcc
     2461 tcagtcaccc ggccctgagt tttggcgtcg gtatagcagc tcgattcatt gagtcgacga
     2521 gcattgtgaa aacacttgga gtacagggcg ttgcttggcg cgcgcggagt ttgcctgtgt
     2581 gttaggcgaa cgccggttgt cgcacagccg cggactattg gatcgcccag tggtcggaaa
     2641 tcaggcgtga atagttgggg ctctgtttac cgtttggaca tccgctccct taaagattcc
     2701 tcagtacaga tcccttgggg aatcgaacga tggagaggcg ccaacacgac atgttgaatc
     2761 gttttgcaac agtggctgct gtttgcatgc tggtcccggt ctggaccgct gcggtcgctc
     2821 tggcggacga cgatccgcct ccggccgtcc cggctggcga cgttggaccc ccgccagaca
     2881 ccggcgttgt cccggccgga ccgcccggaa tcgcacacgc ctccgagggg cggacgctga
     2941 ccgtcgtcgc gaaggacgag acgcagctgc ccatagcgcc gttgacgacc tcgctggcat
     3001 cgcgcgactg gctggtgggt gccaccttca ccggatcggg tatgggttcg gcgaagggcg
     3061 gatcgctcga ggtcggatac cagatcggct gcgcgatcga gatggacaag gtcaagctga
     3121 acggcagcat cgggatcggc tccaacgccg ggtcgttcac cggcaatgcc ctgaacttcc
     3181 cgaccgcgct taccttccca atccagggtc agatcgaggt cgagcccagg cccggcacgg
     3241 tcacaaacgt cgtggtcgac aagaagtcgt acaaaggaaa ctcgacccgg gtcaccgtca
     3301 aggacatcca catcaaggtc gacaactgcg tcggcgcctc gtccctgcgg tcctacgcga
     3361 ttctgaccag ctcgaatggc gataccgacg acgtcgttgc ctactacggc gtcacaaagg
     3421 tgttctgagt gaggatgatt cgtccccatg tgatcagttc tcaccgtgtg acggtccaga
     3481 agcacctcac cgccggcttg gcggcgggca tgctggccgt cggcgcggcg gtcgcctccg
     3541 cggcgaccgc cgccgcggag gagccgccgg cgccggcgcc ggcacccgct gaccccgcgg
     3601 cacccgccgc cgcagcaccc tctggattcc tgcctccggg cgggggagta cagaacttcc
     3661 tccccgaagg aggactggcg ccgggctcgg gatatgactt cctcttaggc cagg
//
LOCUS       DASYLX010001113         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3594032,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001113 DASYLX010000000
VERSION     DASYLX010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594032"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1839)
                     /locus_tag="VGP27_26045"
     CDS             complement(148..1839)
                     /locus_tag="VGP27_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEV7854798.1"
                     /translation="MYVVATAGHVDHGKSTLVQRLTGMWPDRLAEERRRGLTIDLGFA
                     WTEIDGRQFAFVDVPGHERFVANMLAGVGAAAPSENPVMFVVAATEGWMPQSDEHLAA
                     IEALDVRDVLVVISKADLADPGPAIEQVRLRLPDAPIVLGTDLDAVRAELVKLVDRLP
                     APDVGGDVRLWVDRSFTVRGAGTVVTGTLAAGTVRAGDELEHNGRRVTVRGLQSLERD
                     HSEVAAVARVAVNLRGVDRRHIGRGDTLRTPGAWLDTAEIDVALRRAGDLHRELVLHI
                     GSAAVPVHVRPLGDAAARLRLSRPLPLRIGDIGLLRDPGEHRIAAGIEVLDVRPLPLR
                     RRGAARDRADELATGRARPPVCARANELRAMGFPLTGLRIGDWVVDPQWWSERRQHLM
                     TAVTEWSSRHDIAAGMPLEAVRQQVGLPAAELVAELLDGTGLEVADGRVRRPGATLPP
                     RVDKAIKAVEEWLAAEPFLAPGADELSDLDLGPKELAAAVRTGRLARIADGVVLGPDV
                     YDRAADTLRTLPQPFTVADAKRAMNTTRRVAVPLLEELDRRRVTRRADDGTRRVV"
     gene            complement(1827..>2576)
                     /locus_tag="VGP27_26050"
     CDS             complement(1827..>2576)
                     /locus_tag="VGP27_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEV7854799.1"
                     /translation="MASTGSRFREVGTTNRTHLRDYLDAIGPDTGFVLKVHPSNYSVS
                     GFTSTVSVSELARIEVPVVADIGSGLLTPHPLLPDEPDATTMLRDGADLVTASGDKLL
                     GGPQAGLLFGDAELIERLRRHPAARALRVDKLTLAALEATLVGPPSPVAQALDADVAD
                     LRVRAQRLAERLPDAQAVDSVAAVGGGGAPDVSLPSAAVSLPESCAVSLRTGTPAVVG
                     RLENGRCLLDLRTVPPQDDEALLAAVLACMS"
BASE COUNT          352 a          932 c          948 g          344 t
ORIGIN      
        1 tgcgcggcgt cagggtgagc gagcgcttgg actgggcggc cttgccgcga ccgagccggt
       61 cgtcgcctgt gagctgcctg tacacaggcc acgactcgac cagtttcctg aagtccatcg
      121 gcgtctccta ccctgcggct atcccgatca aaccactctt cgagtgccgt cgtcggcacg
      181 cctcgtgacg cgtcgtctgt cgagttcctc cagcagcgga acggccacgc gccgggtggt
      241 gttcatggcc cgcttggcgt cggcgacggt gaacggctgc ggcagcgtcc tgagggtgtc
      301 ggcggcgcgg tcgtacacgt cggggcccag cacgacgccg tcggcgatgc gggcgaggcg
      361 gccggtgcgg acggccgcgg caagctcttt cggacccaag tcgaggtcgg aaagttcgtc
      421 ggccccgggc gccaggaacg gctcggcggc caaccactcc tcgaccgcct tgatcgcctt
      481 gtcgacgcgc ggcggcagcg tcgcgcccgg tcggcggacc cggccgtcgg cgacctcgag
      541 gccggtgccg tcgagcagtt cggccaccaa ctcggcggca ggcaggccga cttgctgccg
      601 gacggcttcg agcggcatac ccgcggcgat gtcgtggcgg ctcgaccact ccgtcacggc
      661 cgtcatcagg tgttgacggc gttccgacca ccactgcggg tcgacgaccc agtcgccgat
      721 gcgcaggccg gtcaacggaa agcccatggc gcgcaactcg tttgcccgcg cgcacaccgg
      781 tgggcgggca cgaccggtgg ccagctcgtc ggcccggtcc cgtgccgctc ctcggcgccg
      841 cagcggcagc ggccgcacgt cgagcacctc gatgcccgcc gcgatgcggt gctcacctgg
      901 atcgcgcagc aagccgatgt cgccgatgcg cagcggcagc ggccgcgaca accgcagtcg
      961 ggcggcggcg tcgccgagcg gacgcacgtg caccggcacc gccgccgacc cgatgtgcag
     1021 caccagttcg cggtgcaagt cgccggccct gcgcagcgcg acgtcgatct cggcggtgtc
     1081 cagccacgcg cccggcgtcc ggagggtgtc gccgcggccg atgtgccgac ggtcgacgcc
     1141 gcgcaggttg accgcgacgc gcgccaccgc ggcaacctcg ctgtggtcgc gttcgagcga
     1201 ctgcagcccc cggacggtga cgcgacggcc attgtgttcg agttcgtcgc cggcgcggac
     1261 ggtgcccgcc gcgagcgttc cggtgacgac cgtgccggcg ccgcggacgg tgaacgaccg
     1321 gtccacccac agccgcacgt cgccgccgac gtccggcgcg ggtaaccggt cgacgagctt
     1381 caccagttcc gcccgcacgg cgtcgaggtc cgtgccgagc acgatcggtg cgtcgggcaa
     1441 ccgcaggcgg acctgctcga tggcgggccc cgggtcggcg aggtccgcct tgctgatgac
     1501 cacgagcacg tcgcggacgt cgagtgcctc gatcgcggcg aggtgctcgt cggactgcgg
     1561 catccagccc tcggtggccg cgacgacgaa catcaccggg ttttccgagg gggcggccgc
     1621 ccccacgccg gcgagcatgt tcgcgacgaa gcgctcgtgg cccggcacgt cgacgaacgc
     1681 gaactgccgc ccgtcgatct cggtccacgc gaagccgagg tcgatcgtca ggccgcggcg
     1741 ccgctcctcg gcgagccggt caggccacat gccggtgagc cgctgcacca gcgtcgactt
     1801 gccgtgatcg acatggcccg cggtcgctac gacatacatg ccagcaccgc cgcgagcagt
     1861 gcctcgtcgt cctgcggcgg gaccgtgcgc agatcgagca ggcagcggcc gttctccagc
     1921 cggccgacga ccgcaggcgt gccggtgcgc agcgacacgg cgcacgactc gggcaggctc
     1981 accgcggcgc tgggcaacga cacatccggc gcgccgccgc caccgacggc cgcgacggaa
     2041 tcgaccgcct gagcgtccgg cagtcgttcg gcgagccgct gcgcgcggac ccgaaggtcg
     2101 gccacgtcgg cgtcgagggc ctgcgcgacg ggcgagggcg gcccgacgag cgtggcctcc
     2161 agcgcggcga gggtgagctt gtcgacgcgc agggcgcggg cggccgggtg gcgccgcaac
     2221 cgctcgatca gttcggcgtc gccgaacaac agcccggcct gcggcccgcc gagcagcttg
     2281 tcgccgctgg cggtgacgag atcggcgccg tcgcgcagca tggtcgtcgc gtcgggttcg
     2341 tcgggcagca gcggatgggg ggtgagcagg cccgagccga tgtcggcgac gaccggcacc
     2401 tctattcggg ccagctccga cactgacacc gtcgacgtga agccgctcac ggagtagttc
     2461 gacgggtgca ccttgagcac gaagccggtg tcggggccga tggcgtcgag gtagtcgcgc
     2521 agatgggtgc ggttcgtcgt gccgacctcg cggaaccgcg agcccgtcga tgccat
//
LOCUS       DASYLX010001114         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3816054,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001114 DASYLX010000000
VERSION     DASYLX010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816054"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGP27_26055"
     CDS             <1..306
                     /locus_tag="VGP27_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7854800.1"
                     /translation="EIVGQSLDMIIPEKLRDRHWRGYRETMLTGYTRYGDKLLSVPAT
                     HRDGRRLSVEFSVALLRDDADQIVGISAIMREVSERREAEKALRTKLTELESRVGSA"
     gene            complement(354..1775)
                     /gene="pyk"
                     /locus_tag="VGP27_26060"
     CDS             complement(354..1775)
                     /gene="pyk"
                     /locus_tag="VGP27_26060"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV7854801.1"
                     /translation="MVNRRAKIVCTLGPATSTAPRLAELIDAGMDVARLNFSHSTQAE
                     HSVVYDAVRQISAERGRPVGVLADLQGPKIRLGRFAAGPVVWATGERIVITTAECRGD
                     HDRVSTTYGGLAADVRPGDRLLVDDGKIDLRVVDVNGDDITCEVLGGGPVSDHKGISL
                     PGVAVSVPPLSEKDIEDLKFALELGVDMVAMSFVRAPEEVKLARAVMDEVGKWAPIMA
                     KLEKPEAVSDLKAIVDAFDGLMVARGDLGVEMPLEQVPLVQKRAIGLCRQAAKPVIVA
                     TQMLDSMISDRRPTRAEVSDVANAIFDGADAVMLSAETSVGAYPAHTVATMARIVAAA
                     EDAPYEEITPEWAAEEGDAGVGDAVAAAACELGRRLGAKVLCCFTRTGDTALRLARQR
                     SPLPLLAFAHDESVRARLAFSWGIESAVLAPATTAETLPTEVSRGLAAMGMRHPGSLV
                     VVVSGSRSGVSGCTDSVRVLRIE"
     gene            complement(1784..>2242)
                     /locus_tag="VGP27_26065"
     CDS             complement(1784..>2242)
                     /locus_tag="VGP27_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7854802.1"
                     /translation="KAPVEADLRNDIWIKLMGNVAFNPLSALTRATMVELCQHPGTRR
                     VVIQLMEETLDIAARVGSTPDISIEKRLRGAENVGHHKTSMLQDLESGKQLELDAIVT
                     AVVEMADLTGAAAPTLRAIHAATDLLARTCAPASSQAIGTPEKETSPVMS"
BASE COUNT          373 a          769 c          726 g          374 t
ORIGIN      
        1 gagatcgtgg gccagagcct cgacatgatc atccccgaga agctgcggga ccggcactgg
       61 aggggctacc gagagacgat gctcaccgga tacacccgct acggggacaa gctgttgagc
      121 gtgccggcca ctcaccgcga cgggcgccgg ctgtcggtcg agttcagtgt ggcgttactg
      181 cgcgacgacg ccgaccagat cgtcgggata tcggcgatca tgcgcgaggt cagcgagcgc
      241 cgggaagccg agaaggcgtt gcgcaccaag ctgaccgaac tcgagtctcg ggtcggctcg
      301 gcctgaacat gcgccgagcg ggtgcaccga ccggaatgac cgagccggca gcgtcattcg
      361 atgcgcagca cgcgcaccga gtcggtgcac cccgaaacgc cgctgcggct gcccgacacc
      421 accacgacca gacttcccgg atgccgcatg cccatggccg ccaagccccg gctcacctcg
      481 gtcggcaggg tttcggccgt ggtcgctggt gccagcactg cagactcgat gccccaggag
      541 aatgccaacc gagcccgtac cgattcgtcg tgcgcgaagg ccagtagcgg cagcggcgat
      601 cgctgccgcg ccaggcgcag ggccgtgtcg ccggtacgag tgaagcagca cagcaccttc
      661 gcgccgagcc gtcggcccaa ttcgcaggcg gccgcggcaa ccgcgtcgcc aacgccggca
      721 tcgccttcct cggcagccca ctccggtgtg atctcctcgt atggcgcgtc ttcggccgca
      781 gcgacgatac gcgccatcgt ggccaccgtg tgggccggat acgccccaac gctcgtctcg
      841 gcagacaaca tgactgcgtc cgccccgtcg aagatggcgt tggctacgtc ggagacctcg
      901 gcccgggtgg ggcgtcgatc agagatcatc gaatccagca tctgggtcgc cacgatcacc
      961 ggtttggcgg cctggcgaca caggccgatg gcgcgcttct gcaccagcgg gacctgctcc
     1021 agcggcatct ccacaccgag atccccgcgg gcgaccatca gtccgtcgaa cgcatccacg
     1081 atcgccttca ggtccgaaac cgcctccggc ttctcgagtt tcgccatgat gggtgcccac
     1141 ttgccgacct cgtccatgac ggcgcgcgcg agcttcacct cctcgggagc ccggacgaac
     1201 gacatggcta ccatgtcgac cccgagctcg agggcgaact tcagatcctc aatgtccttc
     1261 tcggacaggg gcgggacgct caccgcgacg cccggcagcg agatgccttt gtggtcactt
     1321 accggaccac cccctaaaac ctcgcaggtg atgtcgtcgc cattgacgtc gaccacccgc
     1381 aggtcgatct ttccgtcgtc gacgagcagc cgatcacccg gccgcacatc ggccgccagc
     1441 ccgccgtatg tcgtggagac ccggtcgtga tcgccccggc attcggcggt ggtgatcacg
     1501 atgcgctcac cggtggccca aaccactggg cctgcggcga accggcccag ccgtatcttg
     1561 ggtccctgca gatcggccag caccccgacc ggccgaccgc gttcggcgga gatctgacgg
     1621 accgcgtcgt agacgaccga gtgctcggct tgggtgctgt ggctgaaatt cagccgcgcg
     1681 acatccattc cggcgtcgat gagttcggcc agtcgcggtg cggtgctggt ggcgggccct
     1741 aatgtgcaga cgatcttggc gcgacggttc acgattggtc ctctcaggac ataacgggcg
     1801 aagtttcctt ctccggcgtg ccgatggcct gcgacgatgc aggcgcgcac gtgcgtgcga
     1861 gcagatcggt ggcggcatgg atggcgcgca gagtgggagc ggcggcgccg gtcagatctg
     1921 ccatctccac caccgcggtc acgatggcat cgagttccaa ttgcttgccc gattcgaggt
     1981 cttgcagcat ggaggtcttg tggtgtccca cgttctcggc tccacgcagc cgcttctcga
     2041 tggaaatgtc cggcgtgctg cccaccctgg cagcgatgtc gagcgtctct tccatcagct
     2101 ggatgaccac gcgccgggtg ccggggtgct gacacaactc gaccatcgtc gctctggtca
     2161 gcgcgctgag cggattgaac gcgacgttgc ccatgagctt gatccagatg tcgttgcgca
     2221 ggtcggcctc gaccggcgcc tt
//
LOCUS       DASYLX010001115         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2716581,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001115 DASYLX010000000
VERSION     DASYLX010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716581"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGP27_26070"
     CDS             <1..537
                     /locus_tag="VGP27_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7854803.1"
                     /translation="VTPEQAARLGAWGVIASVQPNFDTLWGGDDGMYAQRLGVDRALR
                     LNPLALLASQGVPLAFGSDSPVTGMNPWVTVRAAVRHKTQASAISVRAAFSAATRGAW
                     RAAGVRDGITGTLVPGAAASYAVWQVDQLEVSAPDNAVQRWSTDPRSRVPALPRLGVD
                     DPLPRCRQTVHRGAVIHG"
     gene            530..>1905
                     /gene="lnt"
                     /locus_tag="VGP27_26075"
     CDS             530..>1905
                     /gene="lnt"
                     /locus_tag="VGP27_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV7854804.1"
                     /translation="MVKRTPHRRRGRAEDDTDSFPVFDYERADEGDGDGEATSADTTA
                     DESAAKRQVPAWVQRFGTAVVARLSHISAALVGGLLLCLSFPPFGWWYTAIVAFALLA
                     WVLTRDTTKLVGGFGYGFLFGAAFYIPLLPWTGAMVGLLPWLVLSLVQAVFPALFGLG
                     AVAVRRLTAWPIWFAGLWALAEWAKSTVPFGGFPWGVVGFGQTNSPLLSLAQIGGAPL
                     VSFAVTLLGFALAALAFEIYDWWRGGEEGKPADSAPPSVVLPGACICLVLIATAAISP
                     AVRHSGAGAADEPSVNVAAVQGNVPQLGLDFNAQRRAVLDNHVRETMRLADDVRAGRA
                     PQPLFVIWPENSSDIDPLANQDAADQIGVAVEAIKAPILVGGVLKGVGYTPDNPVSTN
                     SIIVWNPGTGPGDRHDKQIVQPFGEYLPWRSFFKHFSPYAERAGYFAPGSGNGVVEAA
                     GVPIGVST"
BASE COUNT          259 a          649 c          675 g          322 t
ORIGIN      
        1 gtcacacccg agcaggcggc gcggctcggc gcgtgggggg tcatcgcaag cgtgcaaccc
       61 aacttcgaca cgctgtgggg cggcgacgac ggaatgtatg cgcagcgtct cggcgtggac
      121 cgggctttgc ggctcaaccc gctcgcgctg ttagcatccc aaggcgtgcc actcgccttc
      181 ggctccgatt cgccggtaac cggcatgaat ccctgggtca ccgtccgtgc ggcggtcagg
      241 cacaagaccc aagccagcgc gatttcggtg cgtgcggcgt tctcggccgc gaccaggggc
      301 gcgtggcgcg cggccggggt gcgcgacggc atcaccggca cgctcgttcc cggcgcagcc
      361 gcctcgtacg cggtgtggca agtcgaccag ttagaggtca gcgcccccga caacgcagtg
      421 caacgctggt ccaccgaccc gcgttcgcga gtgcccgcgc tgccccgtct cggcgtcgac
      481 gacccgctgc cgcgatgccg tcagaccgtg caccgaggtg cggtgatcca tggttaagcg
      541 gacgccacac cggcgacgtg gccgcgcaga ggacgacacc gactcgttcc cggttttcga
      601 ctacgaacgc gccgacgagg gcgacggcga cggcgaggca acgtccgccg acacgacagc
      661 cgacgagtcg gccgcgaagc gccaggtgcc cgcatgggtc cagcggttcg gcacggccgt
      721 ggtggcccgg ctctcgcaca tcagcgccgc gctcgtcggc gggctgctgc tctgcttgag
      781 cttcccgccg ttcggctggt ggtacaccgc gatcgtcgcg ttcgcactgc tggcctgggt
      841 gctgaccagg gacaccacca agctcgtcgg cggattcggc tacggcttcc ttttcggggc
      901 ggccttctac ataccgctgt tgccgtggac cggcgcgatg gtcgggctcc tgccatggct
      961 ggtgctctcg ctcgtgcagg ccgtgttccc tgctctgttc ggcctcggcg ctgtcgcggt
     1021 acgcaggctg accgcgtggc ccatctggtt cgccggcctt tgggcactcg ccgagtgggc
     1081 gaagtcgacg gtgccgttcg gcggattccc ttggggcgta gtcggtttcg gccagacaaa
     1141 cagtccgctg ttgtcgctgg cgcaaatcgg tggggcgccg ctggtgtcgt ttgcggtgac
     1201 gttgctcggc ttcgcgctcg ctgctctggc cttcgagatc tacgactggt ggcgcggcgg
     1261 cgaagagggc aagcccgcgg actcggcgcc gccctcggtg gtcctgcccg gcgcttgcat
     1321 ctgtctcgtg ctgatcgcca ccgcagcgat ctcgcccgcc gtcaggcatt ccggcgcggg
     1381 ggccgccgac gaaccgtcgg tgaacgtggc ggccgtgcag ggcaatgtgc cgcagctggg
     1441 gctggacttc aacgcgcagc gccgtgcggt gctcgacaac cacgtgcggg agacgatgcg
     1501 gctcgccgat gacgtgcggg cgggccgtgc gccgcagccg ttgttcgtca tctggccgga
     1561 gaactcgtcg gacatcgacc cgttggccaa tcaggacgcc gccgaccaga tcggggttgc
     1621 ggtggaggcg atcaaggcgc cgatcctggt cggtggagtg ctcaaaggcg tgggctacac
     1681 cccggacaac ccggtctcga ccaactcgat catcgtgtgg aatcccggca ccggccccgg
     1741 cgaccggcac gacaagcaga tcgtccagcc gttcggcgaa tacctgccat ggcgcagctt
     1801 cttcaagcac ttctcgccgt atgccgagcg tgcggggtac ttcgcacccg gcagcgggaa
     1861 cggcgtcgtg gaggccgccg gcgtgccgat cggggtatcg acctg
//
LOCUS       DASYLX010001116        51360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3816102,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001116 DASYLX010000000
VERSION     DASYLX010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 51360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51360
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816102"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGP27_26080"
     CDS             <1..283
                     /locus_tag="VGP27_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854805.1"
                     /translation="FPNDASHSAVTIGHNNAAMKDQSGTCGDAVFTSAALGAASFGVD
                     PTWSGEPSKANVSLYTRGVPDGSDSVFEPDSVHQAMIGVNDGGPDQTGC"
     gene            complement(754..2379)
                     /locus_tag="VGP27_26085"
     CDS             complement(754..2379)
                     /locus_tag="VGP27_26085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7854806.1"
                     /translation="MPLAEGAVFAGYAVVRLLGSGGMGEVYLVEHPRMPRREALKILP
                     ADVSANPEFRARFNREADLAATLWHPHIVAVHDRGEDDDQLWIAMDFVDGPDTSQLLR
                     DRYPKGMPTGDACEMVRAVAEALDYAHGGGLLHRDVKPANVLMAQPHSGRRRILLSDF
                     GIARRLDDISGLTATNMTVGTLNYAAPEQLMGETIDGRADQYALAATAFHWLTGSAPF
                     ENSNPAVVISRHLNAPPPLLEPSRPDLAGVDDVLAKALAKQPDERFSSCTDFADALTE
                     AVRGRSNVRSAPTESALPTQPGRLKGVGTVAAEFDRRPAGPTWMRANIRVPVSLAALL
                     VCVLAVAVFAIVRTTDGSSSARSTTSSTTASSASLEPRVTDAPAPPPAVLPPPPAAPT
                     AAAISYAIPACYSSYDPPTERPANAVIEYCGDGGTQLIYMTWTTWGPDGAEGKGVFAV
                     KSCQPDCAHGPTIQYPVVIHAANPFPIPSNVGCGPSTAVYSDLTLAFPTASVPQNING
                     MSVNSSYEGLPAIHFTTNQNDSSATNLGSTGCW"
     gene            complement(2678..4588)
                     /locus_tag="VGP27_26090"
     CDS             complement(2678..4588)
                     /locus_tag="VGP27_26090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV7854807.1"
                     /translation="MTTTDGTDLGADTRRLPDIKSPDAYPAAADLTQSCSLVMKGGIT
                     SGVIYPRAVCQIATTRRLARIGGTSAGAIAAAAAAAAEYARSTGTDASTAVGYPRLAR
                     LPDELVKATDGHTMLYQLFQPQRRTRAVYRVVSVWIGRGGKSSKLIRSVVPLLGALLP
                     APGLIAAIAAIALLGGALSMSNWLAVVLAAALVVVILVAMTAISLWKRINQDLPGNHF
                     GLCTGMGSPTALTEWLTAEINQIAGKSPDGDPLTFGDLHHAGVELAMLTTDLSSGTQN
                     ELPFRSRVWAFDPVEFAEFFPPHIVRWLRKHPPEPEGDVDSTTFSRFEEHTPPLCPLP
                     PPDQMPVIVGVRMSLSFPPLLSTVPLHAIDYTRTATGAPTISDPDTAPPTEHPIIRHR
                     FSDGGITSNFPLTFFDESLPKRPTFGINLVEISDADLSPTQSDNVWMPTGNGQGILTR
                     PTIIDSTTAFVGAILDTMQNWTDSMQAHVPGYRGRIVAVEHTKHEGGLNLDMDEPTIM
                     RLAKRGQCAGQRTETFDFTNHRWVRFRSLLQTMQEFLAPAQSQLAADPDDLQPTYTQM
                     INDIPSNPSSYRNPWNDDTRAAFTDVKAALSALADKYQSVDQESPADHPLIIEGAPKP
                     HPRLQVRPRPLS"
     gene            4632..5150
                     /locus_tag="VGP27_26095"
     CDS             4632..5150
                     /locus_tag="VGP27_26095"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7854808.1"
                     /translation="MDKVDHVQPTKADPSVVLLNSTSNIYKAYGSELDTTLGRWVRET
                     DNIKIKLNQFVSYIHLKFILKDPLGSDPMMVTGSANFSNASTRENDENMLIIRGDRRV
                     AYIYFTEFNRLWGHYYYRSVVEQTKKTEPPPGQGKPHNYQDLWENTDWQLDYAPGKLR
                     TKWVQQYIDMTV"
     gene            5230..6096
                     /locus_tag="VGP27_26100"
     CDS             5230..6096
                     /locus_tag="VGP27_26100"
                     /inference="COORDINATES: protein motif:HMM:NF039441.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6424 family protein"
                     /protein_id="HEV7854809.1"
                     /translation="MTGTHPRSENFPWTIVVVDHPPRKESTEYIASRKLMRKIVAQVD
                     DWVLGPPPYEDHHGGSVWVKDEEGWLCTQLPLGIEWSAQFAADPAKVDKLRVMAARII
                     TAFPGTVEAYEEYGYDSERSLLKTEITSARHVARWTDSIFNASMPVPRAVHTGVLPKG
                     AGYHHYPKPIVDIDHFRRDDLPLFVDDDDGHPWSSSLLRRGRTRVGDCWQPARIQPWL
                     KGAASRTHPDGLEGQIHYLDDLAFGYGGRSDFDAAPPAGQPTFRRREPRDPTILSADN
                     PLVRKALSRGPK"
     gene            6506..6631
                     /locus_tag="VGP27_26105"
     CDS             6506..6631
                     /locus_tag="VGP27_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854810.1"
                     /translation="MPLNRTGPHALLLHRTTTVEAVVIDTDDATASLDSPRLRLH"
     gene            6846..7181
                     /locus_tag="VGP27_26110"
     CDS             6846..7181
                     /locus_tag="VGP27_26110"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7854811.1"
                     /translation="MKNVYGLPLVLACLMGASSLSPAAASADTLGPFIDYLDSNGVDV
                     STIDAQNADVELGKAICGMYPPALRVGANPNDGVMAALRPRHTEAEAAMWLVGSVNYV
                     CPQYVYLLP"
     gene            7263..7643
                     /locus_tag="VGP27_26115"
     CDS             7263..7643
                     /locus_tag="VGP27_26115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854812.1"
                     /translation="MRMLAKGVAALLFTVGAMVGAPLASADPGYGFPANCDTVPIGFF
                     KAKLRTVCDTPIRPDGSWSRKRVFWIPAHHVSAQSDCSSSTYSSHCTFTDAYDVDDTI
                     VDGPDDYVVFPDNVLPDEPGHLPA"
     gene            8191..8808
                     /locus_tag="VGP27_26120"
     CDS             8191..8808
                     /locus_tag="VGP27_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854813.1"
                     /translation="MAEVDRRADKTVEKILVGTMNALSRRGAHKLSVSDICESSGVAR
                     GTFYRYFTSKEDVLAALARHFEDGLAAAFAAAIEVNPDPAVRVQVVLDTVIAYCAAGG
                     DLVRMLDVAPSFTLEFIRDTFPHLLDVVTDALGPAAAESPLVTGGALTTRQLGDLFLR
                     SVISMLFLPGNSSYEVPDVVTSLFRTDAEGGTTRRRKRRTAAKAS"
     gene            complement(9001..9391)
                     /locus_tag="VGP27_26125"
                     /pseudo
     CDS             complement(9001..9391)
                     /locus_tag="VGP27_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
     gene            9397..9645
                     /locus_tag="VGP27_26130"
     CDS             9397..9645
                     /locus_tag="VGP27_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854814.1"
                     /translation="MGSNRVEFRDQPPAVDLSRETRTQDAVLVGALCPTMRVWFTNAR
                     TTAGLSVADASAGGGTVATAETCFYCAPGTTVPRVNDL"
     gene            9918..10319
                     /locus_tag="VGP27_26135"
     CDS             9918..10319
                     /locus_tag="VGP27_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV7854815.1"
                     /translation="MELTRTTINLERVGIVGPDGQPTAEARYDWELPVETLCRLYELM
                     VVTRDLDGEFVNLQRQGELALFPSGRGQEAARIGATACLHKTDWLFPQYRRYPEAAIL
                     GMGSPKTRAVVVDDAPNSPIPLRDRSIPRIA"
     gene            10352..11494
                     /locus_tag="VGP27_26140"
     CDS             10352..11494
                     /locus_tag="VGP27_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854816.1"
                     /translation="MKRYLYDYDHEAFRATVRAFYLDQVAPDCTDWGVGAPPRHFWRA
                     AGKLGLLGIQVPKEYGGGGRGGFLFNVILTEECRRAGIQLDSLRMHTDIAMPFFLRVA
                     NAEQQSRWLPQLVSGDAVAALATADVGAGCDDKAMTVRAIRDGRNYIVYGSRTLVCDG
                     ADADLIVTPVTTNPAAGRDGLSLLVVNGSSPRLTQGPKLDKVGARTRGMAALIFDDVV
                     VPAENLLGEEGRGFSYMIENVAQERLSIAVNSHSAAVKELYDTIEYTKERKAFGATVS
                     SFQHTKFVLAACATDIEAGQSLLDRALVLHESGELSTADAAMVKLFCTEMCGRVGDRC
                     AQLHGDCSEMLERHPISAAYVDAGVSPSYGASSEVMKLIIAQSLGV"
     gene            11639..11814
                     /locus_tag="VGP27_26145"
                     /pseudo
     CDS             11639..11814
                     /locus_tag="VGP27_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(11836..12780)
                     /locus_tag="VGP27_26150"
     CDS             complement(11836..12780)
                     /locus_tag="VGP27_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7854817.1"
                     /translation="MGIVPTPATSDADSWRATKTVNTHETEKMVEAVIGAGIEILMAT
                     GTFGECASLTHDEWLTFTTCVADVVADRVPFFAGVTTLNTRDTIARGREALAAGADGL
                     FIGRPMWLPLDDAATVRFYRDICEALPSFPVVIYDNPAAFKGKISVEAYHQLAALPAI
                     VATKHAGGPTLVRDIQAVGDKVAVLPLDSDWYDAARRFPDLATACWSGNVACAPSPLA
                     ALSRAILSRDWATAETISKKVNWALAPQFGGDMSRFMDYSIPMGRARFRGAGLIDPGP
                     SRPPYTEAPTEMIEGGEETGRRWAELEKEFRDRLSETA"
     gene            complement(12823..13950)
                     /locus_tag="VGP27_26155"
     CDS             complement(12823..13950)
                     /locus_tag="VGP27_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7854818.1"
                     /translation="MTTTRESGEKLGDLDKQLDEWHLRGQWQYDAQLVQLTDGPIPAG
                     VPFLWERDLVDRALTQMCQVMSSDTGRRNFTFINPAADVNGTTRTLAMGMQITLPGEV
                     AWAHRHSISALRFVVDGSAHLYTAVDGEKMPMLDGDLIITPAYSWHDHHNDSDKRGVW
                     LDILDVPFMGYLNQFFFEPFGGSTQPLHRTSADFISNRSTNIRPAWERQQDSRIPFRY
                     AWSEVRDTLQRYADCDGSPFDGVILHYAHPVTGGATLPTIDCFAQLLRPGLRTERHRH
                     TASAIYYVVEGQGTTEVGEQTLDWSVGDSFVVPNWMWHAHINRSSTTDAVLFCSTDAP
                     ILEALGMYREEPYPSFATKPYPPIPGDLSRWSEPTERPAKK"
     gene            14739..15386
                     /locus_tag="VGP27_26160"
     CDS             14739..15386
                     /locus_tag="VGP27_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854819.1"
                     /translation="MAANNETVGRILDAAVTTLARRGVCKLSMSEVSAEAGISRGTLY
                     HYFDNKEDLLRAIAQHVEGVFEKAVVTAVNKCPELDVRLQVVIEAMTEVGGTHPEALQ
                     VLAIEPAFGIDFLQQAFPQFVAILEQLLTPALELTPAVRTMGLTSRQLSEMILRIVMS
                     AYLIPTNDLADVSRAIVALPYLHQTPLHTQLAHLRSSHKMRPIGGRRPRSGSSTF"
     gene            complement(15647..15892)
                     /locus_tag="VGP27_26165"
     CDS             complement(15647..15892)
                     /locus_tag="VGP27_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7854820.1"
                     /translation="MAEHENTLLVTVDRSKCCGYTLCAVEAPGVYSIDDEGFAVAPDS
                     VPAELEDQARTGARACPATAIALLRRRPGGQVSPEGG"
     gene            16639..17820
                     /locus_tag="VGP27_26170"
     CDS             16639..17820
                     /locus_tag="VGP27_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854821.1"
                     /translation="MTTIDAETTTVLDAIRKLTPEIAERAGEIETAKVVPADLVERIA
                     AAGAFRMFVPHQFGGEEMSLPAAMAVIEEVSRADASTGWTVMIGADFAPVFGRFPQHI
                     LDGEIYAEGPDAMARGAFAPKGIAVRADGGYLVKGQWPLASGSYDHEWVMGNCVVLEN
                     GQPRISELGVPDTRLVMVPTEEAQFLDTWDSLGLRGTSSNDMVIDDVFVPEQHTAQFF
                     GPASVDIPLFRPPIRLAIGPTHVAVVMGIASGALEDLRVLAKTKRPAFNPGMRLAEDP
                     VFQFRLGQLDIRLAAVRALAEKDVGAIWDAAVAGEPATDLSAVRQRATVARAHTECVE
                     IVNEAFGLAGSNAMYNTSTLQRRLRDVRTAAQHAAASAEIYTIVGALLVGEDVPRAAL
                     A"
     gene            17838..18284
                     /locus_tag="VGP27_26175"
     CDS             17838..18284
                     /locus_tag="VGP27_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854822.1"
                     /translation="MTTNATTAPAMTAFCELSRLNNAFAYFMDNGEFESMIELFTPDG
                     VFDRAGIVHRGHEELRQGMRERPKVTTRHLLTNFHLTSVGDDSAEAVVCAMVYHGPPS
                     QAGEPVVYATDNGRVIEFHDKYTKTSGRWRISSRIARAIFTPKVWP"
     gene            18312..18707
                     /locus_tag="VGP27_26180"
     CDS             18312..18707
                     /locus_tag="VGP27_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854823.1"
                     /translation="MAENHHDVLATVDKFVNALATEDAGTLDALFTDDAVVWHNYDQV
                     GQPAREALAALAGLAALQPRFEVVGRDVVDTVCIQRHVVHITLPGAEAASIPVIQRIS
                     VADRRIRRIDEYTDSAQMVAAVQALQAGV"
     gene            18740..19315
                     /locus_tag="VGP27_26185"
     CDS             18740..19315
                     /locus_tag="VGP27_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7854824.1"
                     /translation="MPGITDKFTGEPILKFQRIGHGTLEVVDLARSRRFYEEVLGLQV
                     IQSSSRSIMIRLGTDQVYAVVETGRESSMPMMAHNGFDVGSEDEVYAAHKKLLEIKDQ
                     WGIRQITQPRHMHGDTSFHFADPDGNWWEVTEVRKNGYVADFEDPDWDITGMHEFDEE
                     RGNVHFNHTHDPEFRARVRDVRASIDAKASQ"
     gene            19312..20142
                     /locus_tag="VGP27_26190"
     CDS             19312..20142
                     /locus_tag="VGP27_26190"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7854825.1"
                     /translation="MSRLDGKVALVTGAGKGIGRGIARRFAREGAKVVVAELDPDAGK
                     QVAEDLDELGGEGLFISTDVSQKADIERAVGTAQLAFGGLDILVNNAIALTPDVLLEQ
                     KTDEMFEQTMRVGLWSVWWGMQCAFPIMKARGGGRIINFYSMDADDGNWVHADYNTAK
                     GGVQALTRTAGFQWARHNILVNAIAPIAASAAFEQMAAENPAWAAAIPAMIPIGRIGD
                     PEEDIAPVVGFLVSDEARYITGATIPVDGGLHIPRVNTRPPDLSVFDSLSNNSELPTS
                     "
     gene            20170..21561
                     /locus_tag="VGP27_26195"
     CDS             20170..21561
                     /locus_tag="VGP27_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7854826.1"
                     /translation="MRHTSAGLVLSDGTPLSELIDKDYREVSLRVLNDPELYQLELKH
                     LFAKAWTVVAHEDEIPDVGDYVLRYVGEDQVIVSRAAGGSFTAALNVCTHRAGKVCRF
                     EAGNAKQFQCAYHGWAYKSDGTFLAAPVAREEMHGLLRTKDELSLTKARVDTYCGMIF
                     VTFDENAPSLRDYLGPMAWYWDLMFDRCPRGMTVVGHPQRFIIPSNWKSAAEQFAGDI
                     FHTLSLHRSMQELGILSGGEESAEPAMAGAHASWEGHYVRCFDIREDHFVNALKGKDL
                     AALSPMERLRLAPPAGMPAEMVDALPDRFDDKQLKVLAEFPPQVGQLFPNIAAIAMPF
                     QMPDGTPSAFFSWRVWVPKGPDNFEMFSWTLVERDASVQLRDSMNLMTSAVFGISGFV
                     EVDDTNTWPMQTQAARGVLGRQQKLRYQAITGENKPADWPGPAHIYAGFPKDDGQWNF
                     WLRYSEFMEGRAW"
     gene            21558..22151
                     /locus_tag="VGP27_26200"
     CDS             21558..22151
                     /locus_tag="VGP27_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEV7854827.1"
                     /translation="MTTEATEEVMVQSSSMVMPETGYPAPPGARVPVRDPRHWEIVGF
                     LEDESALLDDDDFTGWLGLLAPEVVYRAPVRTTKDHRARSIFDAESYHYNENIMTLSL
                     KLMRVVQTDSAWSENPLSRTHRIVHKVRVYETDNPDEYQVYSSIVLLRSRNDEPGLEI
                     LPARRLDTIRTGDWKLTSRTVYFDQTTLSVRNLAVYL"
     gene            22197..22781
                     /locus_tag="VGP27_26205"
     CDS             22197..22781
                     /locus_tag="VGP27_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV7854828.1"
                     /translation="MSSKTAELPGYLPGDWRIDPQRSTLKFSIGHLGLHTVDGTLAIS
                     GEFVMADEPEGSSVSAVIDLASVHTGSHGRDKSARSSSLLDVGNYPTGHYRSTAVVPA
                     APGGNPRTFVLEGELTLLDVTRPVALDISLQEPASTDGRPCPVIAGRGRFARRDFGLV
                     YRIRPQFLDRAMGSTIDVELTMQGLPAPPRGPQP"
     gene            22784..23632
                     /locus_tag="VGP27_26210"
     CDS             22784..23632
                     /locus_tag="VGP27_26210"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7854829.1"
                     /translation="MARTRTHQGDDCWSLVDVAAGTVQPIRGEVSQWGPAAAEGCTDF
                     ETAGAPMSLEDVRLLPPITPTSTIVGVGMNYWSHLEKLGVTERPSSTLGFLKPQVAII
                     GHNDEIANPFITDQLDFEVELVVVLAQPIREVSSNLTDYVLGYTVGNDVSARDAAPAP
                     MGGLDLFSVKTLTGTTPVGPWITTKDEFGGSGEVDVTISLRVNSEERQRDRTGKMIFG
                     IEECLEYIVARMALNPGDIVFTGTTDGVGFEDGRYLQAGDVVEAEVEGVGVLRNVVGA
                     KQSPTR"
     gene            complement(23922..24659)
                     /locus_tag="VGP27_26215"
     CDS             complement(23922..24659)
                     /locus_tag="VGP27_26215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7854830.1"
                     /translation="MSPALLEALERQLNAANADESVAVTVITSGLKVFSAGADATWMA
                     GEADRLGVEGLTAEFTKSMDRFRALCVAMRRSPILFLAALNGHTLAGGLELAAACDLR
                     YVADTGTLKIGVPEMDLFGALPSGGGGVQFLNRLMQPSRALQFILDAKPISPREAYSV
                     GLVDRVVPADKLLSESTSFASAVSAKAGRIGVAAAKRAVLGGAELPLYEALEFDRSLH
                     WDAVRRGNFPRGVRSFIDQFASSRRTS"
     gene            complement(24736..26487)
                     /locus_tag="VGP27_26220"
     CDS             complement(24736..26487)
                     /locus_tag="VGP27_26220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7854831.1"
                     /translation="MKDRYPPTSVPAHCLVAPQDQPDYTEPNHDLVGAWANVGYALSD
                     AHVCAGHGDDLAMIHADTARSYTFSAIAEVSTLLAQGLMALGVVVGDRIALRGPNVPE
                     LIIGMLAVWKAGGVVVPTPLQAQPEELRFFLDDTSARFLVVVAATGDDLAVVAEAVAG
                     SPIEEVFTYGDTPSGAGPYRDLRILTDATEIHAAPLPTTPSDAVAVLWHTGGTTGQPK
                     GCYHTHRRFLLGGYALGAVTAAARGQRWAAAAPAGHALGFMYHTTGTLLHGSTTVLIE
                     NFRDPQRVVAAIADFQVNVFTAITATWARMIDVLDENGRDLPSLRRAYAMWQSASSAD
                     VRMRWQKRGITLINNYGSTAFATWPMTPRHDEDFPAASLGRPLPGYIVAAVQRTGSGL
                     QVIETGRGQMAVRGLTGLTYWNRPELQRRDVVDGWSICDDLIEFDGTGNAAYLGRTDY
                     LISTSGYKVAPVEVENVLSTHTAVREVAVVGAPDPIRQEIVVAFIAVRPGVPTNDELA
                     TELQAHVRNRLSPYKAPRRFHFVTELPRDPVGKIRGKVLQTWAADGAAPAPSTPDQKP
                     RSLQGGDVIDPDPTARH"
     gene            complement(26487..28064)
                     /locus_tag="VGP27_26225"
     CDS             complement(26487..28064)
                     /locus_tag="VGP27_26225"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEV7854832.1"
                     /translation="MARLFGGGLRDGDARTAIHDVAIIGMGPVGTALAGLLGRRGLDV
                     IVLEQDADVFALPRAAHIDHTGLRAVQELGCLEQVLPSMTLNPGIEFVSADRSRLMWM
                     PAGQTTWSGVPTSVYFHQPGFDRALRAAVAALPTVTVHLEAEVVDVTQDTNEVSLQTA
                     WRGGNQESEPVGTVRARYAVACDGAASPVREMAAMRLEDLGFHERWLVVDLLLGDADR
                     ALPGHALYVCDPARPHVFIPMPGRRHRVEFMVHSGEDTAAITEPTAVASVLRELVGED
                     FGTVERAAVYTFHGLVAQRWRSSRLLLAGDAAHQMPPFLGQGMCSGLRDATNLAWKID
                     RVLRGAPIDLLDTYEIERSPHVREVIKAAIDYGAIVGITDPQAARERDEAIRRDHRSL
                     FFGLPSLQPGPMVLEGGGGLFGQPRVGGKPLDDAVGQRFLVLGRHDEALGSTSRWWAD
                     VVGAWVTTIADLPQPDPALAEWLDSQGADVAVVRPDRYVLAAVAGTLDQITADVSALL
                     QPAENTAARLDTSTRTR"
     gene            complement(28081..29268)
                     /locus_tag="VGP27_26230"
     CDS             complement(28081..29268)
                     /locus_tag="VGP27_26230"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7854833.1"
                     /translation="MTTAAAIIGVGMTPFGRFPQRSLKGLGGEAVHAALADASMSPAD
                     IDMAFVANAMASIVTGQVSIVGQTVLRDNGFSGIPVYNIDNACAGSSSALNLAVHAVR
                     AGAATRVLVLGVEKLFARSRETTYAALNGAADIDFVAESGVDATRESVFVAAVYPPRL
                     REYADRHGLSADTLAAVAVKNRSHAGLNPMAQYRQPLTAKEVLAARSVVEPITALMCA
                     PISDGATAVVVTAGVSVRPEHRPVWIRGSAVGMAAAGRQGSTIGGVARRAYADAGITP
                     HQVSVAEVHDSIAFNELVAYEELGFCDPGAGARLVADGLTSLGGQLPVNTSGGLESRG
                     HPVAATGLAQVIELAHQLRGEAGGRQVPDAQIGVAENAGGYAGGDTAAVAVTVLGRTA
                     ADD"
     gene            complement(29265..30062)
                     /locus_tag="VGP27_26235"
     CDS             complement(29265..30062)
                     /locus_tag="VGP27_26235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854834.1"
                     /translation="MSAANGGSARKRTASGMSFVKRGVGKPVLLLHGWCLDGSLWAYE
                     EDELFGDHQVFVPDLPGFGRSDTMPEPYDMNAHTDAVIELLVEQGLRDVLVIGFAFGA
                     AVALTVAARDDSRVSGVIAVGVPSCKVFPAERMARAMRRDWPEFARRSAAVLCKQPQS
                     DATLSWLERVFGATPLRSALQGVALLGEFDPLPLCESVSKPVLFVHGQDDDVIPIREA
                     QACAESAPRGSLRVLPECGHLVVLDQRHVLHDTIVGFEDTLTRSDRP"
     gene            complement(30238..30702)
                     /locus_tag="VGP27_26240"
     CDS             complement(30238..30702)
                     /locus_tag="VGP27_26240"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV7854835.1"
                     /translation="MPFFYDDLAVGRVFKLPARTVTESDLVGFAMLSGDWNPIHTDRE
                     FATDTIYGQPVVYGLLSLIMMTGLMDRSGIFSGSAIAMLDITEWRFEKAVFVGDTLSA
                     TVEIVSKRLTSSGDRGVVDRLFRITNQRGEVVQIGHIGLMIRLSPQQTEVAR"
     gene            complement(31474..32913)
                     /locus_tag="VGP27_26245"
     CDS             complement(31474..32913)
                     /locus_tag="VGP27_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854836.1"
                     /translation="MYLSRQVKLQLTFFTIVTLIAGGTMAFKYLDLPSVFFGIGHYEV
                     TVNLIASGGLYRNGNVTYRGVAVGRVQDVRLGPNGVEAVLSLNSDTKIPADLVAQVRS
                     QTAVGELSLELNPRSGDGPSLKNGDVIPVDRTALPPDINAVLADTNSGLQAIPHDDLN
                     TAVDEAYTAVGGLGPELKRIVDGTTTLAADARANLSAITTVIDQSKPILDTQTDTSDS
                     IQAWAANLAQITSQLRDQNPALNGLLNNGPAAGDEVRNLLDRLQPTLPVVLANLVSLE
                     QVAITYQPSLEQLLVLLPKNVEEMQAPLVADRNTDHKGIYLSLNLNANLPPPCTTGFL
                     PAQQMRPPSDVDYPERPAGMLYCRVPQDSSLNVRGARNIPCMTRPGKRAPTVQMCESD
                     EQYVPLNDGNNWKGDPNATLSGQPIPQPLPETPPAASPAPPPIAIVEYDPNTGTYTGP
                     DGKQYTQTDLTHGANHQKWQDLLVPPNGS"
     gene            complement(32915..34033)
                     /locus_tag="VGP27_26250"
     CDS             complement(32915..34033)
                     /locus_tag="VGP27_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854837.1"
                     /translation="MTAVVLVGAVAVTLLSGCGWRGLNSLPLPGSAGGGPGAFTIQAQ
                     MPEVSNLQPNQRVRVGDVDVGTITRIERQDWHALVTIRLNGDISLPANSTATLGQTSL
                     LGSLHIELAPPSDGMSRGKLHQGSVLPLSSGKAYPSTEQTLAAASLLLNNGGLGQIQD
                     ITEALSTAFAGREGDLRSLLSQIDNFVSQLNDQTSEIIGATDSFNRLVGQFAEQKPVI
                     DEGLRTIPDALAILSREQDNLIEAIDQLGKLSALAEDSVQQTKDALVQELKDLGPVLQ
                     SLADAGRSLTRSLNFLPTFPFTKDTLTKWFRGDYANISLIVDLTLSRLDSSFLTGTRF
                     EGTLTKLEMQWGRTIGQLPSPYTLGNPLIAPYRYDQGP"
     gene            complement(34054..35385)
                     /locus_tag="VGP27_26255"
     CDS             complement(34054..35385)
                     /locus_tag="VGP27_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854838.1"
                     /translation="MISVRNARIAMAIVLVFIAAGAGYFLVWSGERTDRIEVTAYFDN
                     SIGLFPGDDVRIRGVPVGKIGKIEAQPTQVKITFWFDSKYKVPADAKAVILSPTLVTA
                     RAIQLTPPYRDGPTLQSNAVISKQRTAVPVEWDDVRLQLQRLANTLQPTKAGGVSTLG
                     SFIHTTADNLRGQGAAIRDTIIKLSQAISTLGDHSDDIFSTVKNLSVLVSALQDSTDL
                     LRQLNGNLASVTGLLADDPGEVATAVKDLNDVVSEVTSFVTDNREALGTTSDKLASVS
                     SALTQSLDDVKQLLHVLPTLAANATNIYQPAQGTLSGAPVLNNFANPITFLCGAIQAA
                     SRLGAEQSAKLCVQYLAPIVKNRQYNFPPLGFNPFVGANARPNELTYSEDWMRPDYVP
                     QQPTPPAAPPAPAGLSALPPEAPLAAESAQPHQTDPAAGLPGIMVVPGAGS"
     gene            complement(35382..36704)
                     /locus_tag="VGP27_26260"
     CDS             complement(35382..36704)
                     /locus_tag="VGP27_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854839.1"
                     /translation="MKSFRERNPIIVGAISLAVVGATTVGALNYSNLPFISSGSTYSA
                     YFEEVGDLSTGAPVQVSGLRSGQVKDISLTPRGVLITFTVADNIRLGERTEAAIKSKS
                     LLGTKVFEVTPRGSGHLSAAIPVERTTSPFQLPDALGDLTATISGLNTTQLSDALQTL
                     SDTLQDTPPQLRVAVDGVARFSQTIDRRDAELRNLLSNASKATTVLAERTDEIVQLLH
                     DTNAVLAALQGQSAALDNISGNVSALAQQLQGFISDNRATLKPALDKLNGVLATVDNR
                     KDQIQQSIKGLNSYALSLGESLSGGPFFKVYVANLLPGQFLQPFVDAAFSDLGLDPNV
                     LLPSQRVDPPTGQPGTPALPVPYPRTGQGGEPNLSLPEAITGNPGDPGYPYREPLPAP
                     APGGPPPGPPAQQAPDPNASTPPNPSAVLVPTPGELAPTTPPAEGAGQ"
     gene            complement(36701..37729)
                     /locus_tag="VGP27_26265"
     CDS             complement(36701..37729)
                     /locus_tag="VGP27_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854840.1"
                     /translation="MRRNLTGLLWRLAAFLSVCVLGAAALITIYAQVRFNQEKSYNAI
                     FSTVSGLENGNFVRIAGVEVGKVKGITISDDASALVKFSVDDSVVLTGGSRAVIRYND
                     LIGGRYLALEQGPGVTMPLPPGATIPLERTAPALDLDALIGGFRPLLRGLDPDQVNKL
                     TGQLIAGFQGQGPTIGSILEQAAALTNTLADRDALIGQVVINANTVLGSLGLGSDKLG
                     VAVDSLSHLVHGLQGRSQDISGAVASANAGAATIADLLAQARPALQKTVQEADRTAAV
                     VLADHDYLDDFLNTLPDAYRVLGRQALTGDFFNFYLCDISLKVNGKGGQPVYVKLVGQ
                     DSGRCTPK"
     gene            complement(37783..39180)
                     /locus_tag="VGP27_26270"
     CDS             complement(37783..39180)
                     /locus_tag="VGP27_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEV7854841.1"
                     /translation="MQKRPRLPRIDPSWWALLLVLGVFGATALSLGAFNRSFSPGVAV
                     TLTADRSGLVMEPYAKVKMRGVQIGRVETVASTADSATLELDIDPGQIENIPANVKAR
                     INATSLFGAKYVDLIPPTDPSPQRLSAGAVLKSENVAIEVNTVFQNLVELIKQVDPYK
                     LNAVLSALAGGVRGKGEQIGQSITESNDVLLALNSRTDTLRADFQALANVSDTYSDAA
                     QNIIDTVAAASTTAATVTAQAAQLDKLLVSVAGLSQRGIELVRPNKENFVKTINLLEP
                     TTSLLMKYNPELTCLLVGADRTGAELSPVYGGSNGKSVVMDATVLLADDPYRYPDNLP
                     IVAAKGGPGGKPGCGSLPDVAQNWPVRYLVTDTGFGTGLDMRPNPGIGFPGYANYLPV
                     TRAVPEPPSIRYPGGPAPGPIPYPGAPPYGAPQYGPDGAPLYPGVPAPPPSPETPASN
                     QSAVASTDPGAPAPP"
     gene            complement(39185..39994)
                     /locus_tag="VGP27_26275"
     CDS             complement(39185..39994)
                     /locus_tag="VGP27_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854842.1"
                     /translation="MLGTARAEWRRLGEQTRFYGKVLGSIADVVIHYRAELLRQIAGM
                     SAGTGSLAVIGGTVAVMAFLTMSTSGVLSSQGYNQLSEVGVDAVTGFVSAFASTRLVT
                     PGTAAFAFAATIGAGTTAQLGAMRINEEIDALEVMGIRSIAYLASTRVAAGVIVAVPL
                     FCVALLLGYTSGQFVTTALYGQSSGVYGHYFSTFFIPADAVRSFIVTLAQVTSIMLVH
                     TYYGFTASGGPAGVGEAVGRATRASLIVSAFVIMLVTLALYGQTGNFNFAG"
     gene            complement(40042..40812)
                     /locus_tag="VGP27_26280"
     CDS             complement(40042..40812)
                     /locus_tag="VGP27_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7854843.1"
                     /translation="MVGYERPALAPVRALGDFSATALDIFVAMVKWPFAWREFLEQTW
                     FVARVSFLPSIVLAIPFVVLSVFTFNVLLIEFGAGDLSGTGASISAVNQIGPFVTVLV
                     VAGSGATAVCADLGARTIREELDALRVMGIDPVRTVLAPRVLAVTLVSLMISSLVTMT
                     GLVGGFLFGVYFQHVTPGAFVAGMTLLTDLSDVLVSMVKATVFGLAAGLIAVYQGTTV
                     SGGPAGVGNAVNQTVVYSFVLLFMVNLVITAIGFEVTK"
     gene            41418..42290
                     /locus_tag="VGP27_26285"
     CDS             41418..42290
                     /locus_tag="VGP27_26285"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854844.1"
                     /translation="MMTASTDGALSVPKPPAAGSPHDWPVLEDWPLSKEATVGRLMGK
                     TAVISGVASGIGALAWRRFCEEGASVVGIDINESAGKQLADELRANGHDGEFFTADVT
                     SKEAIDSLAALIRDRFGGLDILYNNAGINLARPLLETTEAEWDNVHNVDLKSTFLMMK
                     AFAPLMQDRPASIINTASMGGLVAYDNMAAYCAAKGGLIMLTKAAALELAPAVRVNVI
                     CPTLIDTPMSRRFVGDLPLEFDQVWKEFEGGHPLGRIGKPEDIVPLAVYLASDESLMM
                     TGVALPVDGGWSIR"
     gene            complement(42736..43725)
                     /locus_tag="VGP27_26290"
     CDS             complement(42736..43725)
                     /locus_tag="VGP27_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7854845.1"
                     /translation="MPLALDLACWDYDRTRALADGRVRVEGVDLNYIPMEMPESFHRM
                     FRNAEFDASEMSFAWYVGSLFRERRNMVAIPVFPSRMFRHSGIYVNTKSGIVTPDDLI
                     GKRVGVPEYQQTAGVWIRGILADYYGVPVDSVRYFQGGLNQPGRLESALRRPEAIQLD
                     TIPSDETLSAMLARGELDAVYTAHAPDGFADGAPYVRRLFPDYRREEQMYYERTGIFP
                     IMHTVVVRAELLTSHPWLAQSLTKAFEAAKKIAVDDLFEPATAKTALPWLTAEAEATE
                     RLFGSRDFWPYGAEANQHVIDAFLRYMHEQGLVPRRLTVEELFPQSTLRVART"
     gene            44088..45362
                     /locus_tag="VGP27_26295"
     CDS             44088..45362
                     /locus_tag="VGP27_26295"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854846.1"
                     /translation="METPTGCPYHRRDEPTPDSIATVSRYRDVEEIFRSPAMAPFLHD
                     GTEQFRGGTVRLIDGSTHRLRRRTMGQLLRGQGEVRFREEVLMPTIRRNLARVLSSAT
                     HGRPSTDMVQFARVAFFQLAAALIGLDGVNTTEDAEQLRQFAEPIQVAMRSWYMTDDR
                     TAVMAQGIEVRDQFRERYFKPALERRVALVATAKTAEQEAELPNDFLTLVARGVDPDW
                     SVNRDLALRETITDFINAGTFSSSFTLVHALDECLTWAERHPDETDRLLDEQFLAAGV
                     AEALRLHPIVPLSYRTASEAVTLKSGRTFHAGEHVCLEVGLANRDPDVFGADVDSFIP
                     GRPVPPGVYPYGVSFGTGRHMCYGQPVILGTGGTTGSHVQILKALFGAGVQRHPSRPP
                     RLSGDFGRMNALWETYPIELEPVSAVYAESSM"
     gene            45524..46111
                     /locus_tag="VGP27_26300"
     CDS             45524..46111
                     /locus_tag="VGP27_26300"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEV7854847.1"
                     /translation="MSEAQASKIAIFVGHIQQPIVGMAPEGYLDRLAETLAAARAAGV
                     SVIYAKLSFRAGYLELPDHDPMRAMVDADNLFIGGLSSTIDEAVKPLEGDIVIDAVRP
                     SAFAYTELEPILRSRGINSIVLTGISTGGVVMGTLVDARSRDFAVTVLSDLCVESNPS
                     GQVALLEAFTYPWAATITDASTWVTELAEFTGAVG"
     gene            46428..47324
                     /locus_tag="VGP27_26305"
     CDS             46428..47324
                     /locus_tag="VGP27_26305"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV7854848.1"
                     /translation="MSSDKPSATVAKISPTTWEPTPFTSSAVAFRPQELVISLVGEYV
                     EPGGRVWSGGLVQLLDCLGFTTPGARVALNRLVSRGLFDVVKDGRKVFYTATARLVEL
                     LNEGHQQTFWFRYSQKPWRGDWTLVWYTIPEEQLLKRRRLSRRLVFLGFGALQDGTWL
                     APHDRSEELWTAVEDLQLRDYVVVVVGAAPAWASRQTMIRRAWNVDEASARYTAIVEG
                     FRQFSNRRVLATVTPERAFIVRTHLIEAIRQVAPLDPKLPDADTGFRWHRRLALEVFK
                     DVDAALQPMASEFFNEKTRPHS"
     gene            complement(47942..48544)
                     /locus_tag="VGP27_26310"
     CDS             complement(47942..48544)
                     /locus_tag="VGP27_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854849.1"
                     /translation="MATRATTEGRIFNGALHAIARQGPKKLTMSDVAAESGVSAGTLY
                     RYFKNKEEILAALGDHFVMKLQEVLQQAIAEDPEPANRLRVVVDVMLRYWQDNPATLH
                     IGQLEAGFTVNYIKSVWPQLSAVLHNALEPVLADSAAIRNGSVTVDEVVELIVRMAFS
                     HYFIPTRDYLDLRDLLVTLGRSAGLEPKKSGARPHRKAAS"
     gene            48956..49135
                     /locus_tag="VGP27_26315"
     CDS             48956..49135
                     /locus_tag="VGP27_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854850.1"
                     /translation="MAASQSATQASRIDAETDSQPAELDSDTDIAAEADEVTDDESAP
                     AAPASRPVSGVRVAR"
     gene            complement(49209..49610)
                     /locus_tag="VGP27_26320"
     CDS             complement(49209..49610)
                     /locus_tag="VGP27_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854851.1"
                     /translation="MAFDLTHSWQFLSFINGLPKFSPARQLQTVLCPPEGINGECGTT
                     LLNRRMGGASDRFEARDQPARSDLVKSHLDAGGGVAGRISADDDGLVNAPVDESRMRR
                     TSVSTSWKSNVVRLIAAWRPTSTKSSRCALA"
     gene            49590..>51360
                     /locus_tag="VGP27_26325"
     CDS             49590..>51360
                     /locus_tag="VGP27_26325"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7854852.1"
                     /translation="MRQIESQVLIVGGGGAGLTTSVFLADLGVEAMLIERHDSTSSLP
                     KAHYLNQRTMEIFALHGMADAVYAKSAPRANNAKIKWLSSVGGDGPLDRNVLAEADVM
                     GGGDTASMYNAKGFRPPTHIPQFRLEPIIRQIAHQRRPAGVLFRHELRSFTQDDNGVI
                     AIVRDLLVEEDIEVRCQYLLGADGGRTVGPQLGVSMQGPTGMADFVSVWFSADLSSYL
                     TDSDSVMRVIFPPGQVAQGKYTCILVAYGPTRWDGHSEEWGLGLMRSPDDPDDVDEKS
                     IVPIIREFLKIDVPLQVHKVTHWLLETTVANRFSFDRVHLLGDAAHKQTPGAGLGLNS
                     AIQDAHNISWKLALVLKGSAPPSLLSSYEDERRPVITQNVGWSMFSLTNHLLVVSAMG
                     VNLAAPPEANEAAIAELISDTRTGASKRARLAEVFRTQRVEYQAHDMDMGFNYPVGAV
                     VDDGSPSPWRDPMGAEYRPTTRPGCRLPHTWLEYQGTRRSTHELIPMGGMLALTSDEP
                     LAQQWRDAVAKVSADTGVPIRTVRIGPRGDAFDPTNNWIALREVGPKGIVLVRPDGHV
                     GFRAINVPPNPADTIAAAVHHILS"
BASE COUNT         9682 a        15501 c        16373 g         9804 t
ORIGIN      
        1 cttcccgaac gacgcgtcgc attccgccgt cacgattggg cacaacaacg cggcgatgaa
       61 ggatcagagc ggcacgtgcg gcgacgcggt gttcacgtca gcggcgctgg gtgccgcctc
      121 cttcggcgtg gaccccactt ggagcggtga accctccaag gccaacgtca gcctgtacac
      181 ccgtggtgtg cccgacgggt cggacagcgt cttcgagcca gactcggtcc atcaggcgat
      241 gatcggggtg aacgacggag gccccgacca gaccggctgc tgaaccgcca gcctgggacg
      301 ggagtggtgg tactaacgct atggaatcgg cggctttgga gcaggtctca gcagccgagt
      361 gccatcatcg aggtcggtcc tcggcagcaa gttcggtcat catgatcttc acgatcttgt
      421 tagtgacggg ctggccgtcg atccttgctg gcggatcacg gccgctccgc gactattacg
      481 caggcagcct tcgccggcgt catgcacacg ccaccggcgg ttacggtgtc agatcctctg
      541 caccagcgga ctccgaagct gatccgacgg aatagccatt cccggaccgc aacagccacg
      601 aggaagcgag gctcggcgca agtcgggcgc accgaagttg ctgctgacgc tatcggtggc
      661 ggcaagctcc cgtcgagatt tatggcaccg agactccggg ctcactccgg gattcaccgg
      721 cgctgtgcgc catccgggtg cctcatggcc taactaccag cagccggtgg atccgagatt
      781 ggttgcgctg gaatcgttct ggttggttgt gaagtggatg gcgggtagtc cttcataact
      841 gctgttgacg ctcattccgt taatgttctg gggcactgag gcagtgggga acgccaacgt
      901 caggtcgctg tacaccgcag tagagggtcc gcagccgacg ttggacggta tagggaaggg
      961 attggcggcg tggatcacca ccgggtactg aatggtgggg ccgtgggcgc agtcgggttg
     1021 gcatgacttg acggcgaaga ctcccttccc ctcggcgccg tcggggcccc atgtcgtcca
     1081 agtcatgtaa atgagttgtg tgccgccgtc tccgcagtac tcgatgacgg cgttcgcggg
     1141 acgttcggtc ggtgggtcat agctcgagta gcaggcggga atggcgtagc tgatggcagc
     1201 ggctgtgggt gccgccggcg ggggaggaag gacggcgggc ggcggtgccg gcgcatctgt
     1261 tacgcgtggc tccagggatg ccgaagaggc cgtcgtggaa gaagtcgtgc tccgtgccga
     1321 cgatgaccca tcggtggtcc gcacgatggc aaacactgcg accgccaaaa cgcagaccag
     1381 cagcgcggcg agcgaaactg ggacgcgaat gttggcacgc atccacgtag gaccggctgg
     1441 tcgccgatca aattcggccg ccactgtccc gacacccttg agtcgtccag gctgcgtggg
     1501 gagggctgac tcggtcggtg cggagcgcac gttggaccgt ccgcgaaccg cctcggtcag
     1561 ggcgtcggcg aagtcggtgc agctggagaa gcgttcgtcg ggctgtttgg cgagggcttt
     1621 ggccaggacg tcgtcgacgc cggccaggtc gggacgtgac ggctcgagga gtggaggtgg
     1681 cgcattcagg tggcgactga tgacgaccgc gggattggag ttctcgaatg gcgccgaccc
     1741 ggtgagccag tgaaacgccg tcgccgccaa tgcgtattga tcggctcgtc cgtcgatcgt
     1801 ctcgcccatg agttgttcgg gcgcagcgta gttcagtgtg ccaaccgtca tgttcgtcgc
     1861 cgtcagcccg ctgatgtcgt cgaggcggcg ggcgatcccg aagtcgctca gcagaattcg
     1921 tcgcctgccc gagtgcggct gggccatcaa cacattggca ggtttgacgt cgcggtgtaa
     1981 aagtccaccg ccatgggcat agtcgagcgc ctcggcgacc gcacggacca tctcgcaagc
     2041 gtccccggtg ggcatgccct tcggataccg gtcgcgcagt agctgtgagg tgtctggtcc
     2101 gtcgacgaag tccatggcga tccacaattg gtcgtcgtcc tcgccgcggt cgtggacggc
     2161 gacgatgtgc ggatgccaca gcgtcgcggc aagatccgcc tcgcggttga accgcgcacg
     2221 gaattcgggg ttggccgaca cgtccgcggg caggatcttg agtgcctcgc ggcggggcat
     2281 acgaggatgc tcgacgagat agacctcgcc catcccgccg gagccgagta gtcggaccac
     2341 ggcgtatccg gcgaagacgg caccctccgc taacggcatg tgcggatcct acggggacgg
     2401 tcggaccgtt gcccccgtgt cggtgccgtc gaccacggcc tggtacgagg tcaagttcgg
     2461 gttgctgcgc ttggtcgtaa ccattgtcga cgtcggcgtc gtcacgtcta gatgagccca
     2521 aggcctggtg caggaatgtc aggttcggtg caacggcttg tgaggcgcag ctctgccagt
     2581 acgcatgcgg tgatctcgcc aagcagaggc gggagaggtc ctgaaatgct cactgacagc
     2641 tgggtcgcct tagcggccct gtggtgcgtc actggagcta ggagagtggc cgggggcgga
     2701 cctggaggcg tggatgcggc tttggtgctc cctcgatgat cagtggatgg tcggccggtg
     2761 attcctggtc gacgctttgg tacttgtcgg cgagtgcgga caaggctgct ttgacatcgg
     2821 tgaatgcggc gcgtgtgtcg tcgttccacg ggttgcggta cgagctcggg tttgacggta
     2881 tgtcgttgat catctgggtg taggtaggtt gcagatcgtc cgggtcggcg gccagctggc
     2941 tttgtgccgg ggcgaggaat tcttgcatcg tctggagcag tgatcggaat cgaacccatc
     3001 ggtggttggt gaaatcgaat gtctccgtgc gttgtccggc gcattgccca cgtttggcaa
     3061 gtcgcatgat cgtgggttcg tccatgtcga gattgagacc gccttcgtgc ttggtgtgct
     3121 cgacggcgac gatgcggccg cggtagccgg ggacgtgagc ctgcatggaa tcggtccagt
     3181 tctgcatcgt gtcgaggatc gcgccgacga aagcggttgt gctgtcgatg atcgtcggtc
     3241 gcgtcaggat cccctgcccg ttcccggtgg gcatccagac attgtccgac tgggtcgggc
     3301 tgaggtcggc gtctgagatt tcgaccaggt tgatgccgaa ggtcggccgc ttcggaaggg
     3361 attcgtcgaa gaaggtcagt ggaaagttgc tggtgatccc gccgtcggag aagcggtggc
     3421 gaatgatcgg atgctcggtg ggtggggcgg tatctgggtc gctgatggtg ggggcgccgg
     3481 ttgcggttcg ggtgtagtcg atcgcgtgca gcggcacggt gctcagcagc ggcgggaagc
     3541 tcaagctcat gcgcacgcca acgatgacgg gcatttgatc gggtgggggc agcgggcaca
     3601 gcggcggcgt gtgctcctcg aacctcgaga acgtcgttga atcgacgtct ccctcgggct
     3661 ctggcgggtg ctttcggagc caacggacaa tgtgtggcgg gaagaactcg gcgaactcga
     3721 ccggatcgaa ggcccacaca cggctccgaa acggtagctc attctgtgtg cctgagctca
     3781 agtctgtggt gagcatggcc aattcgacgc cagcgtgatg aaggtcgccg aaggtcaatg
     3841 gatcaccgtc gggactcttg ccggcgatct gattgatctc ggccgtgagc cattcggtca
     3901 aggccgtggg gctacccatc ccggtacaga gcccgaagtg gttgcccggc aaatcctggt
     3961 tgatccgttt ccacaggctg atcgccgtca tcgcgaccag gatcacgacg acgagcgccg
     4021 ctgcaagcac gacggcaagc cagttactca tggagagcgc gccgccaagg agcgcgatgg
     4081 cagcgattgc ggcgatcagc cccggcgccg gcaacagggc tcccaacagt gggactaccg
     4141 accgaattag cttgctgctc ttgccgcccc gtccaatcca gaccgagacg actcggtaca
     4201 ccgcgcgcgt cctgcgttgc ggctggaaca gttggtaaag catggtgtgg ccgtcggtcg
     4261 ccttgacaag ctcgtcgggc agcctggcga gtcgcgggta tccgactgcg gtgctggcat
     4321 cagtaccggt actgcgtgcg tactcggcgg cagcggcggc tgcggcggcg atggcgccgg
     4381 ccgaggttcc tccgattcgc gccagtcgtc gggtcgtcgc gatttggcac accgcccggg
     4441 ggtagatgac accgctagtg atgccgccct tcatcaccag tgagcaactc tgagtcagat
     4501 cggcggcggc cggatacgca tcgggactct tgatgtccgg aagtcgtctg gtgtcggcgc
     4561 cgaggtccgt gccgtccgtt gttgtcatgg ttctccccag tgccgccagc ggccgactgt
     4621 gttttctcct gttggacaag gtagatcatg tccagccaac caaggccgat ccgtcggtag
     4681 tgctgctcaa ctcgacgtcc aacatctata aggcctacgg gtcagaactc gacacgaccc
     4741 tgggcaggtg ggtccgcgag accgacaaca tcaaaatcaa gctcaaccag ttcgtcagct
     4801 acatccacct gaagttcatt ctgaaggacc cgcttggctc cgatccaatg atggtaactg
     4861 gctcggccaa cttcagtaat gcctcaacgc gagagaacga cgagaacatg ctcatcattc
     4921 gtggcgatcg gcgggtggcc tacatctact tcaccgagtt caaccggctg tggggacact
     4981 attattaccg atccgtggtc gaacagacca agaaaacgga acccccacca ggccagggca
     5041 aaccgcacaa ctatcaggat ttgtgggaga acaccgactg gcagttggac tatgccccgg
     5101 gcaagctccg gaccaagtgg gtgcagcagt acatcgacat gaccgtatga catcgacacc
     5161 tgcctcggtt ccgcaattgc gttggacggt caggctctgc gattgattcg ctgacaaagg
     5221 gggaagagct tgaccgggac gcatccgcga agcgagaact ttccgtggac catcgtcgtc
     5281 gtggaccatc caccacgcaa ggaatccacg gagtacatcg ctagtcgaaa actcatgcga
     5341 aaaatcgtcg cgcaggtcga cgactgggtg ctcggacctc ctccgtacga ggatcaccac
     5401 ggcggctcgg tgtgggtgaa agacgaggag ggctggctct gcactcagtt gccgctcggc
     5461 atcgaatggt cggcccagtt tgcggccgat ccggccaaag tggacaagct gcgggtcatg
     5521 gccgctcgaa tcatcacggc attccccgga accgtcgaag cctacgagga atacggatat
     5581 gactcggaga ggtcgctgct gaagaccgag atcacctcag cgcgacacgt cgcacgatgg
     5641 accgattcga tcttcaacgc ctccatgccc gttccgcgcg ccgtgcacac gggagtcctc
     5701 cccaaagggg ccggctacca tcactacccg aagcccatcg tggacatcga ccacttccgc
     5761 cgcgatgacc ttcctctctt cgtcgatgac gacgacggtc acccctggtc atcgtcccta
     5821 cttcggcgag gccgaactcg agtgggcgat tgctggcagc cggcccggat tcagccttgg
     5881 ctcaaaggtg ccgcttcgcg cacgcacccg gatgggctcg aaggccagat tcactacctc
     5941 gacgacctag cgttcggcta cggcggacgt agcgactttg acgccgcccc gcccgcgggt
     6001 caacccacct ttcggcgtcg tgagccacgc gaccccacca tcctttctgc cgacaatcca
     6061 ctggttcgca aagccctaag ccgtggtccg aagtagtcgt ttgacctatc ctgaccattc
     6121 aagtgctgac atgcgggggc gaattaaata tccggcgtcg ccggagcgca ggaagcaaag
     6181 cgcggcagcg tactaaggga tgtcctggtg gcattttgat tggtgtgtgc gaccagcaga
     6241 ttagcgtccc ctcgggatat tcctagacag tacttcccag gatcgggacg ccagtgctaa
     6301 cttccctcct ccggccagct gctccccgcg gtcaaggctc agccggcgac gtggcagccc
     6361 ccggcaaaag ctttcgcacg accgggcgag gatcgagact tcgtcgcagc cggccggtca
     6421 gattaacgtt ggctgcccag accgcggcat cagtcagctg gatctcagtc gccgacccgc
     6481 ccgccactgg ggagcgggcc gccgaatgcc gttgaataga acgggccccc atgcgctgct
     6541 gctgcaccgc actacgaccg tcgaagcggt cgtcatcgac accgacgacg ccacggccag
     6601 cctcgacagc cctcgactga ggctccatta atgtcccgac tggttagagg catccaaaaa
     6661 ctacttggaa gcggaacgcc gcgtggatct tcctggcgac catctacggg cggtcatccg
     6721 ctgaggttcg tgtcgccatc cgcaacatcg tcggcggaat gacggaggcg gataacccaa
     6781 acgcgtcgtc gaggtaccgg gcggggcgta cattgcaaac ttgcccctca ggagtgcgcc
     6841 cccacatgaa aaatgtttac ggcctgccct tagttctggc gtgcctcatg ggcgccagtt
     6901 cgctaagccc agcagccgca tccgccgata ctttggggcc cttcatcgac tatctggaca
     6961 gcaacggagt agatgtgtcc accatcgatg cgcagaacgc cgacgtcgag ctgggtaaag
     7021 ccatctgtgg aatgtatccg cccgctctga gggtaggtgc aaaccccaac gatggcgtca
     7081 tggcggcgct acgaccacgg cacactgaag ccgaggcggc gatgtggttg gtcggctcgg
     7141 tgaattacgt gtgcccgcag tatgtgtacc tgctcccatg acgcggcgtt ctctcgagcg
     7201 acgcaagcgg acgaatcggc catgacctaa atgacacgct aaaactctgg gggagagaac
     7261 gaatgagaat gctcgccaaa ggcgtcgccg ccctgctgtt cacggttggg gcgatggtcg
     7321 gcgccccgct ggcctcggcc gacccgggct acggcttccc cgcgaattgc gacacggtgc
     7381 cgatcggctt cttcaaggcc aagctgcgca ccgtgtgcga caccccgatc cgccccgacg
     7441 ggagctggtc gcgcaagcgg gtcttctgga taccggcgca ccacgtgtcg gcccagtcgg
     7501 actgctccag cagcacgtac tcgtcgcact gcaccttcac cgacgcctac gacgtggacg
     7561 acacgatcgt cgatggcccc gacgactacg tggtgttccc cgacaacgtg ctgcccgacg
     7621 agccgggaca tctcccggcc tagcgccacg ccgccagttg atcaagctca ccgactgacg
     7681 ccggaagtcc tgggtgttgc tactgcttgc acaacaagat tcgaaagaac tagttggtgc
     7741 agcggctgcg ccccggcggt gtcctcgaaa tccaccgcgg catggcacca gctacccact
     7801 atcgtttcct ggaaatcgct gccgctctcg cctcttactt cggggacaac acccaccgcc
     7861 accgccatcc accaccgcgg tgacacatcc gcctccaccg gtaccccgca gatgcggcga
     7921 tcttcgagca actccatacc gctcgagctt tgctgcgtgt acctggattc cgacgctatt
     7981 ggccctcgag gacttccttg gtgggtttcg tttctacgtg tgttcgctca cagtgccgtg
     8041 tgcgggtccc tgaattgcgc tttccgtgcg acataatcga acgagtagaa cactaattga
     8101 gcaggctgtt cttgtggtga cagagaacgc gggagagcga aggtgttgac acctagattc
     8161 gcttgacttc atcggtaagg agggacgggt atggccgagg ttgatcgacg agcggacaaa
     8221 accgtcgaaa agatcctggt tgggacgatg aacgccttga gccggcgagg cgcgcacaag
     8281 ctctcggtaa gcgatatctg cgagtcgtct ggggttgccc gcggaacctt ctatcggtac
     8341 ttcacgagca aggaagatgt cctggccgcg ctggcccgcc acttcgaaga cggacttgcc
     8401 gcggctttcg ccgccgcgat cgaagtcaat ccagacccgg cggtgcgggt gcaggtcgtc
     8461 ctcgacaccg tcatcgccta ttgcgcggcc ggaggcgatc tagtccggat gctcgacgtc
     8521 gcgccctcgt tcacgttgga gttcatccgc gacacgttcc cccatctcct cgacgtcgtc
     8581 accgacgcac tgggcccggc ggcggcggag tcgccgctgg tgacgggcgg tgcgttgacc
     8641 acacgtcagc tcggagacct gtttttgcgc agcgtcattt cgatgttgtt cctccccggc
     8701 aactcctcct atgaggtgcc ggacgtggtc acgtcgctgt tccgcactga cgccgagggt
     8761 ggaacgacac ggcggcgcaa gcgacgcacg gcggcgaagg cgagctaacg tcggctggga
     8821 gctggcttgg ggaggcatcc gtatgcgaca tcgcgcccga tttattcgcc gttcagcatc
     8881 ggagtctcga cgatctgctc tcagcttgga caaaatggaa ggtaaccagt caactcaacg
     8941 ccgctggtgg caggactggt tgatcgctaa tgtttctgtc atctgactat ctccttcttg
     9001 tcagccctcg gcgtgcggcg ggcggtagtg cgctgttgag ttccgcaact gcccgatgag
     9061 cgcggggcag agttcgttca ccgcccgcga gatcagatag aagcctggta ctcgtcggag
     9121 gtggcgacgt cggccttcac atcggctatg acctgtgcgt aggtccgggc cagacacctt
     9181 gtcgcagatg ccgtttccgt agctcagcgc tgcgtcggcg ttggggaagt ggtagcgggc
     9241 cgcaccatac gttgaccaga taggccaccg cgtcagcgcg aacggtgggg gtggccgcca
     9301 ccgtcgcgac gccgaataac cctgcacacg cgaaaaacag accagtcagc gacttcccta
     9361 ccgtccgccg ggatacggat ccgtgctgca tggtaggtgg gttcaaaccg agtcgaattc
     9421 cgagatcagc caccggccgt cgacctttcg agggagaccc gaacgcagga tgcggtgttg
     9481 gtcggtgcgc tgtgtccaac gatgagggtc tggttcacga acgctaggac gacggcgggg
     9541 ttgtcggtcg ccgacgcctc cgcgggcggc gggacggtgg cgacggcgga gacctgcttc
     9601 tactgcgctc ccgggaccac cgtgccgcgg gtcaacgact tgtaggcgtt caggaacgta
     9661 cggtcactca cccctgcgcg gcttcgacgt tttcccgcgc cttgtcgggc cggtaggacg
     9721 gcggcgccgc ggaccgaggc cgccgccact tcagaaagcc cacagcagag cgctaggtgt
     9781 acgcgctgca cgacgatttt ccgctgacca gacaagggcg ccgtcgtgca ctgaacataa
     9841 agtattaata gtgtctcatc ggtatagtct acggggtgag cgcttccagt gcttcgcgtt
     9901 gtgacaagga gaccgatttg gagctgacgc gcacgaccat caacctcgag cgggttggca
     9961 tcgtcggccc ggacggacag cccaccgcag aagcccggta cgactgggag ctgccggtgg
    10021 agaccttgtg ccggctctac gagttgatgg tcgtcacacg cgatctcgac ggtgaattcg
    10081 tcaacctgca gcggcagggc gaactggcgc tgtttccctc aggccgtggt caggaggccg
    10141 cccggatcgg cgccacggcg tgcctgcaca agaccgattg gctgtttccg cagtaccgcc
    10201 gctatcccga ggcggcgatc ctcgggatgg ggtcaccgaa aacgcgcgcg gtggtggtcg
    10261 atgacgcccc aaatagccca attcctctgc gcgacaggag cattcctcga atagcgtgaa
    10321 atggccctga tggacatgtg aaaggttcgt ggtgaaacgc tacctatacg attacgacca
    10381 cgaggcgttc cgggctacgg tccgcgcctt ctacctcgac caggtggcgc ccgactgcac
    10441 cgactgggga gtcggagcgc caccccggca cttctggagg gctgcaggca aactggggct
    10501 gctcggcatc caggtcccca aagaatatgg cggtggcggg cggggcggct ttctgttcaa
    10561 tgtcatcctc accgaggagt gccggcgcgc cggaatccag ctcgacagcc tccgaatgca
    10621 cacggacatc gccatgcctt ttttcctgcg ggtcgccaac gctgagcagc agagccgctg
    10681 gctgccgcaa ctggtgagcg gtgacgccgt cgctgcgctc gcgacggcag acgttggtgc
    10741 gggttgtgac gataaagcca tgactgtcag ggccattcgc gacggtcgca actacatcgt
    10801 ttacgggtcg aggaccttgg tctgcgacgg ggccgacgcc gacctcatcg ttaccccggt
    10861 gacgaccaac ccggcagccg gccgcgacgg cctgagcttg ctggtcgtca acggcagtag
    10921 tccccgcctc actcagggcc ccaagctgga caaggttggc gccaggaccc gcggcatggc
    10981 cgcgttgatc ttcgacgacg tcgtcgtgcc ggcggagaac ctgctcggcg aggaaggccg
    11041 gggattcagc tacatgatcg agaacgtcgc gcaggagcgg ctgtcgatcg cggtgaacag
    11101 tcactctgcg gccgtgaagg agctgtacga caccatcgaa tacaccaagg aacgcaaggc
    11161 attcggcgct acggtgtcgt cgtttcagca caccaaattc gtgctcgccg cgtgtgccac
    11221 cgacatcgaa gctggccagt cgctactgga ccgcgctctt gtcctacacg aaagcggcga
    11281 actttccaca gccgacgcgg caatggtcaa gctgttctgc acggagatgt gtgggcgggt
    11341 aggggaccgc tgcgcgcagc tgcacggtga ctgcagcgag atgcttgagc gccacccgat
    11401 ctccgctgcc tacgtcgacg ccggggtgtc tccgagctac ggggcttctt ccgaggtgat
    11461 gaagctgatc atcgcccagt ccctcggcgt ctgatggaat cgtcgacggt atctcactga
    11521 tgtcctggac agcagaagtg tgaagttcca cgatccacca cggctgaaga tgcactgctt
    11581 ttcgagttcg aagatgcccg gatcgtggta catatgggcc ggggtcatcc cgagacgcaa
    11641 tcccgcgcat cgggtagcag ccgcgctcaa cgccggcacc atttgggtaa atggcctctt
    11701 tatccgcgat ttgcgcgccc tttttggtgg tgttggtgac tcgggcattg gccgcgaggc
    11761 cggtggcttg gccccgagtt tttcccgccg acggctgtcg tcatggccat ttgacgccgc
    11821 gaatcgccaa taacgtcagg ctgtttccga caacctgtcg cgaaactctt tctccagctc
    11881 tgcccatcga cggccggttt cctcgccgcc ttcgatcatc tcggtcggag cttcggtata
    11941 cggtggtcgg ctgggtccgg gatcgatgag gccggcacca cggaaacggg cacgacccat
    12001 ggggatgctg tagtccatga aacgcgacat gtcaccaccg aactggggtg cgagcgccca
    12061 attgaccttc ttggagattg tttcggcggt cgcccagtcc ctggacagaa tagcccgcga
    12121 taacgccgcc aacggtgatg gtgcacatgc gacgttgccc gaccagcacg cggtcgccag
    12181 atcggggaag cgcctggcgg cgtcatacca atcgctgtca aggggaagga ccgcgacctt
    12241 atctccgacc gcttggatgt cgcgtaccaa cgtcggtccg ccagcgtgtt tggtcgccac
    12301 aatcgcaggt agggcggcca actggtggta tgcctctacg ctgattttgc ccttgaacgc
    12361 cgccgggttg tcatagatga ccaccgggaa acttgggagc gcttcgcaga tatcgcggta
    12421 aaagcgcaca gttgccgcgt cgtcaagtgg cagccacatc gggcggccga taaagagccc
    12481 atcagcgccg gcggcaagtg cttcacgtcc acgcgcgatg gtgtcgcgcg tgttcagggt
    12541 ggtaactccg gcgaagaacg gcaccctgtc tgctacgacg tccgcaacac atgtggtgaa
    12601 ggtgagccat tcgtcgtggg tcagtgacgc gcattcgccg aaggtgcctg tagccatcag
    12661 gatttctatg ccggcaccta ttaccgcttc gaccatcttt tcggtctcat gcgtgttgac
    12721 ggttttggtg gcccgccaac tgtccgcgtc actggtggcc ggtgtgggca cgatgcccac
    12781 aacgcccctg acgtctgatc gggtaatgtt catttcatcc gctcatttct ttgcagggcg
    12841 ctccgtcggt tcactccacc gtgacaaatc gcctggtata gggggataag gcttggtggc
    12901 gaacgaggga tacggctctt cccggtacat tccgagtgcc tcgagaatcg gggcgtcggt
    12961 cgaacagaac agcactgcgt cggtcgtcga ggagcggttg atgtgcgcgt gccacatcca
    13021 gttcggcacg acgaaagagt cgcccaccga ccagtcgagt gtctgctcac ccacctcggt
    13081 cgtgccctgg ccttcaacga cgtaatagat cgcactagcg gtgtggcggt ggcgctccgt
    13141 tcgcagtccc gggcgcagca actgcgcgaa acaatcaatg gtcggcagcg tggcaccacc
    13201 cgtgactggg tgcgcgtaat gcaggattac cccatcgaat gggcttccat cgcaatccgc
    13261 gtacctctgc agggtgtctc tgacttcact ccatgcatat cggaacggga tgcgggagtc
    13321 ctgttgccgt tcccaagccg gacgaatatt ggttgatcgg ttgctgatga agtccgccga
    13381 agtccgatgc agcggctggg tgctgccccc gaacggttcg aagaaaaatt gattcagata
    13441 acccatgaac ggcacatcga ggatgtcgag ccaaacgccg cgcttgtcgc tatcgttgtg
    13501 atggtcgtgc cacgaatatg ccggggtgat gatgaggtcg ccgtccagca tgggcatttt
    13561 ctcgccatcg accgccgtgt aaaggtgggc acttccgtcg accacaaagc gaagtgcact
    13621 aattgagtgg cgatgtgccc acgcaacttc gcccggcagg gtgatctgca tacccatcgc
    13681 cagggttcgg gtggttccgt tgacgtcggc ggccgggttg atgaaagtaa agttccttcg
    13741 gccggtgtcg ctggacatca cctgacacat ctgcgtcaag gctcggtcga cgagatcgcg
    13801 ttcccacagg aacggcacac cggcgggaat cggaccgtca gtcagctgaa ccaactgtgc
    13861 gtcgtattgc cattgaccgc gaaggtgcca ttcgtcaagt tgcttgtcga ggtcaccgag
    13921 tttctcgccc gattcacgcg tcgtggtcat gtccttttgc tccaagttct gtgagaggcg
    13981 cagtgatttt tccgccctgt ctcagtcgcc tcaagtaagg ccgccggcat caagccgtca
    14041 atccgtggcc ggcttttcgc gggggcgccg cgcacagttt cccagtgccc gcgacgaggt
    14101 tgccgcggac accggtaatg cgatcgcagc cgactgcagt aagagccggt gacccgcgtg
    14161 agcgaacccg ccctcaaccg aagggatgga cgtccagcgc ctcgtggtga gtgccacgca
    14221 acctccaggt cgctaggtca gtcatgccgc tagccaacaa ttgatccgcg gaccaagtac
    14281 acacgcgttc cgtgggaacc cagtggcgat gatcgcgcct ggccgaggct agcaccggag
    14341 aggaccgctc acaagacagt tatgacggaa tatgtacaaa ttaacgcaat ccataacatt
    14401 tgatcgagca ttgaagaggt agcgtgctca ggcctgggcg agatcagtgc tgacattcag
    14461 gacggccttc tggggccgcg tccgatctgt tgcgcccagt tggaacgcct ccggtatgcc
    14521 agtgatgttg aggcgcttgg ggttcaaaga ggagcatctg cagcgccccc gatgtcggca
    14581 acatggcccg gcgggcgcca gcgtcggcac tgagcgcccg cagggctcgt tctgttgccg
    14641 tcctgctgca ccctccgcag tcgatgtgac aaaacaaatg ggtggtatgt tgtcgaatac
    14701 gtgccaattt ccgtctgcac tggatatcgg gaggcccgat ggcggcgaac aacgagacag
    14761 tcggacgcat ccttgacgcc gcggtcacaa cgctggctcg ccgcggcgtt tgcaagctat
    14821 cgatgagcga agtcagtgcc gaagcgggta tttcgcgcgg caccctctat cactacttcg
    14881 acaataagga agacttgctg cgcgccatcg cccaacatgt ggaaggcgtc ttcgagaagg
    14941 cagtcgtcac ggcggtcaat aagtgccccg agcttgacgt tcgtctgcag gtcgtcatcg
    15001 aagcaatgac tgaagtcggt ggaacccatc ccgaggctct gcaggtactc gccatagagc
    15061 ccgccttcgg cattgacttc ctacagcagg ctttcccgca atttgttgcc atcctggaac
    15121 aactactcac ccccgccctg gagttgacac cggctgtgcg gacgatgggc ctgaccagtc
    15181 gtcaactctc cgagatgatt ttgcgaatcg tgatgtcggc ctatctcatt ccgaccaacg
    15241 acctagctga cgtttcgcgg gcgatcgtgg ccctgcccta tctgcaccag accccgctcc
    15301 atacgcagtt ggcccacctg cgcagcagcc ataaaatgcg gcctatcggc ggccgacgtc
    15361 cgcggtctgg cagcagcaca ttttgatcgt gcggccgtga cggttggcgt ctggttcggc
    15421 caccaatatg ggtggcgcca gcggattgcg cctcgacgac gggtggttcg ctcgggcggt
    15481 caggttgcgt aacggggagc tcggctccgc caaatagtgg ctagcgagcg gctcatcgcg
    15541 atgacgcggt cgctgaactg tcggttgttt gggtcctttt acccgcgcgg gtatcagcgg
    15601 tcgcggtatc ggcggcagtg gcggagtggc gcccggccag gcgcggctac ccaccttcgg
    15661 gtgagacctg tccgccgggc cgtctgcgga gaagggcgat ggcagtcgca gggcacgcac
    15721 gcgcgcccgt gcgagcctga tcctccaact ccgccggcac gctgtcaggc gcaacggcga
    15781 aaccctcgtc gtcgatcgag tacacgcccg gtgcctcgac ggcacatagc gtgtatccgc
    15841 agcatttgga ccgatcgacg gtgaccagca gcgtgttctc gtgctctgcc atggtgacga
    15901 ccccctcacg ttgacgactc taaaaacgaa acagagctta agcgctcatc gtaagacagt
    15961 atagtcattt agtgtctatt atgactaaca agtgcgatag tgcgcagccg tataacgtgc
    16021 gcttgcgcgg cccctaggcc ggaaccaggc accgcctcgc gtcggtttca gcagcagacg
    16081 ccgccggcct ggcagcccag ctgactcccc ggtcgaggta attcctcacc ggagcgccgc
    16141 tctaccgcgc taacggagtc gccgggcgcg ccgaacgcag aacccggccg cgtggccctt
    16201 tcacaccact aatcgtgctg ctccgaatcc ccttggcccg caccgattcc gtgtcgcacc
    16261 gctggccctt caagcaagcg ggtttcgccg actcgcggtc ggctgacctc gctgcatcgt
    16321 gcaattgtcg gatgggctca gcagctgatc agcgctgatt gcgtcacgga tgcgtccccc
    16381 agcgaaggcg gcaatgctca agctgcattc ctgacggccc aatcggcgga cggtgcgtaa
    16441 ccggagcctt gcagcgtcgg ctgagcaggt agacgcgggt cggcgagggc tcgctttgga
    16501 gatcttcgcc taatgccttg actaggccgt gagcctgaac atatattaca gttagtgttc
    16561 aactagctta gtcttttcaa ccaccgagat cgcactcgtg ctctccgagg cggcgctttc
    16621 tcgatctgga ggcatcgcat gacaaccatt gacgccgaga ccaccaccgt gctcgacgcc
    16681 atccggaaac tcactccaga gatcgccgaa agggccggcg aaatcgagac cgccaaagtc
    16741 gtacctgccg atctggtcga gcggatcgcc gcggcgggcg cctttcggat gttcgtccca
    16801 caccaattcg gcggcgaaga gatgagcctg cccgcagcca tggccgtcat cgaagaagtg
    16861 tcccgtgcgg acgcctcaac cggatggacc gtgatgatcg gtgccgattt cgcgccggta
    16921 ttcggccggt ttcctcagca cattctcgac ggcgagatct acgccgaagg gcccgacgcc
    16981 atggcccggg gagcgttcgc gcccaaaggc atcgccgttc gcgctgacgg tggctacctc
    17041 gtcaagggcc agtggccgct tgccagcggc agctatgacc acgaatgggt aatgggcaat
    17101 tgcgtcgtgc tggagaacgg tcagccgcgg attagcgagc tgggtgtccc ggacacacga
    17161 ctggtcatgg taccgaccga agaggcacag tttcttgaca cctgggattc gttgggcctt
    17221 cggggaacca gcagcaacga catggtgatc gacgatgtgt ttgtgcccga acagcacacc
    17281 gcgcaattct ttggtcccgc gagcgtggat atcccactgt tccggccgcc gatccggttg
    17341 gcgatcggac cgacccacgt tgctgtcgtg atgggaatcg cttccggggc actcgaggac
    17401 cttcgcgtgt tggccaagac caaacgtcca gcattcaacc cggggatgcg cctggcagag
    17461 gatccggtgt ttcagttccg gttggggcaa ctcgacatcc gcctggcggc cgtgcgcgct
    17521 ctggcggaga aggacgttgg cgcgatctgg gacgccgccg tcgcgggcga gcccgccacc
    17581 gatctgagcg ctgtgcgcca gcgcgcgacg gtggcacggg cacacaccga gtgcgtcgag
    17641 atcgtcaatg aggctttcgg attggcggga agcaacgcca tgtacaacac ctcgacgttg
    17701 caacgccgcc tgcgcgacgt ccgcaccgcc gcacagcacg ccgccgccag tgccgagatc
    17761 tacacgatcg tcggtgcgct gctggtgggg gaggacgtcc cgcgggctgc actggcctag
    17821 cgatcgggac cggcacgatg acgacaaacg ccacgacggc accagcgatg acggccttct
    17881 gcgagctcag ccggctgaac aacgccttcg cctacttcat ggacaacggc gagttcgagt
    17941 ccatgatcga actcttcacc cccgacggag ttttcgaccg cgccggcatc gtccaccgcg
    18001 ggcacgagga actccggcaa gggatgcgcg agcgccccaa ggtgacaacc cgtcacctgt
    18061 tgaccaactt tcatctcacg agcgtcggcg acgattcggc cgaggcggtg gtgtgcgcca
    18121 tggtctatca cggtccgccg tcgcaggccg gcgagccggt ggtctatgcc actgacaacg
    18181 gacgggtcat cgagttccac gacaaataca ccaagacctc gggccgttgg cgtatttcat
    18241 ctcggatcgc acgggcgatc ttcaccccga aggtttggcc gtgaccggcc cggacaagga
    18301 gagcgagtct catggcggaa aaccatcacg acgtgttggc cacggtggac aagttcgtca
    18361 acgcgttggc gacggaagac gcgggcaccc tcgacgcgct cttcaccgac gacgcggtcg
    18421 tttggcacaa ttacgaccag gtggggcagc cggcgcggga ggcgctcgcc gcactcgctg
    18481 ggctcgccgc tctgcagccg cgattcgagg tagtcggtcg tgacgtggtg gacaccgtct
    18541 gcattcaacg tcacgtcgtc cacatcaccc tgcccggcgc agaggcggcg tccattccgg
    18601 tcattcagcg gatctcggtg gcggacagaa ggatccggcg catcgacgag tacacggact
    18661 cggcccagat ggtagccgcg gtgcaagccc tgcaagccgg tgtgtgaccg gttcccccaa
    18721 gacgagaaag gtcactaaaa tgccaggaat cacggacaag ttcaccggtg aaccgattct
    18781 gaagtttcag cgcatcggtc acggcactct tgaggtggtc gacctcgcta ggtcgcgccg
    18841 gttctacgaa gaagtccttg gtctgcaggt gatccagagc agtagccggt cgatcatgat
    18901 ccggctcggc accgaccagg tttacgcggt ggtggagacc ggcagggaaa gctcgatgcc
    18961 gatgatggcg cacaacggat tcgacgtcgg cagcgaggac gaagtctatg cggcccacaa
    19021 gaagctcctc gagatcaaag accagtgggg catcaggcag atcactcagc cccgacacat
    19081 gcatggtgac acttctttcc attttgccga tcccgacggc aactggtggg aggtcacgga
    19141 ggtacgcaaa aacggttacg ttgccgactt cgaggatcct gattgggaca taaccggtat
    19201 gcacgaattc gacgaggagc gcggcaacgt ccacttcaat cacacccatg accccgagtt
    19261 ccgtgcccgc gtccgcgacg ttcgcgcttc catcgatgcc aaggcgagcc aatgagccgg
    19321 ctggacggca aggtggcgct ggtaaccggc gccggcaagg gaatcggacg gggcatcgct
    19381 cgcaggttcg cccgcgaggg ggccaaagtg gttgtcgccg aactcgatcc cgacgcgggc
    19441 aagcaagtgg ccgaggacct tgatgaactt ggtggcgagg gactgttcat ctcgaccgac
    19501 gtatcccaga aagccgatat cgagcgcgcg gtcggcaccg cccagctggc attcggtggt
    19561 ttggacattc tcgtgaacaa cgccatcgcc ctaactcccg atgttctgct cgagcagaag
    19621 accgacgaga tgttcgagca gacaatgaga gtcgggctgt ggagcgtgtg gtgggggatg
    19681 cagtgcgcct tcccgataat gaaggcacgc ggcggtggcc ggatcatcaa cttctactct
    19741 atggacgccg acgacggcaa ctgggttcac gctgactaca acacagccaa gggtggggta
    19801 caggcgctga cccggacagc tggatttcag tgggcccggc acaacatctt ggtcaatgcg
    19861 atcgcgccga tcgcggcgtc ggctgcgttt gagcagatgg cggcggagaa cccagcttgg
    19921 gccgcagcga tcccggcgat gatcccgatc ggccgaatcg gtgatcccga agaagacatc
    19981 gcaccggttg tgggcttcct cgtcagtgac gaagcccgct acatcacggg cgccaccata
    20041 ccggtcgatg gcgggctgca cataccacga gtcaacacca gaccgcccga cctctcagtg
    20101 ttcgactcgt tgtccaacaa ttctgaatta ccaaccagct aaaggagaat ggacgtgacc
    20161 attgatgaga tgagacatac ttccgccgga ctggtcctct cggacgggac gccgttgtcg
    20221 gaacttattg ataaggacta ccgggaggtc tcactgcgag tcctgaatga cccggagctc
    20281 taccagctcg aattaaaaca tctgttcgcc aaggcgtgga cggtcgtcgc acacgaggat
    20341 gagatccccg acgtcggtga ctacgttttg cgctacgtcg gcgaagacca ggtcattgtc
    20401 agccgcgccg ctggcggcag tttcaccgcg gcgctgaacg tttgcacaca tcgggctggg
    20461 aaggtatgtc ggttcgaggc cggcaacgcc aagcaattcc agtgcgccta ccacggctgg
    20521 gcgtacaagt ccgacggcac gttccttgcg gcgcccgtcg cgcgggaaga gatgcacggg
    20581 ctgctgcgga ccaaggacga actgagcctg acgaaagcgc gggtggacac ctattgcgga
    20641 atgatctttg tcaccttcga tgagaacgcc ccatccctgc gcgactacct cggcccgatg
    20701 gcctggtact gggacctcat gttcgaccgg tgtccgcgcg gaatgaccgt tgtcggtcat
    20761 ccccagcggt tcatcatccc gtcgaactgg aaaagcgcgg ctgagcagtt cgccggggac
    20821 atcttccaca cgctgtcgct gcatcgatcg atgcaggaac tcgggatcct gtctggcgga
    20881 gaagaatccg cggagccggc gatggcgggc gcgcacgcca gctgggaagg tcattacgtg
    20941 cgttgtttcg acatcaggga agaccacttc gtcaacgcgt tgaaaggcaa ggacctcgct
    21001 gcgttgtcac ccatggagag actgcgcctt gctcctcctg ccggcatgcc cgctgagatg
    21061 gtggacgctc tgccggaccg cttcgacgac aagcagctca aagtccttgc ggaattcccg
    21121 ccgcaggtcg gccagttgtt ccccaacatc gccgcgatcg ctatgccgtt ccagatgccc
    21181 gacggcacac cctcggcgtt cttcagttgg cgggtgtggg ttcccaaggg accggacaac
    21241 ttcgagatgt tctcgtggac cctggtcgaa cgcgacgcgt cggtgcaact gcgagactcg
    21301 atgaacctga tgacgtctgc tgtattcggg atcagcggat tcgtcgaggt ggacgacacc
    21361 aacacctggc cgatgcagac ccaggccgct cgaggggtcc tgggcaggca acagaagctg
    21421 cgctatcagg cgatcaccgg cgagaacaag cccgccgact ggcccggtcc tgcccacatc
    21481 tatgccgggt tccccaagga cgacggccaa tggaactttt ggctccgcta cagcgaattc
    21541 atggaaggac gagcctggtg accaccgaag cgactgagga agtcatggtt caatccagca
    21601 gcatggtaat gccggagacc ggctacccgg cgccgccggg cgcgcgcgtg ccggtgaggg
    21661 acccacgcca ttgggagatc gtcggtttcc tcgaagacga gtccgcactg ctcgacgacg
    21721 acgatttcac cggctggcta ggactgctcg ccccggaagt cgtctaccgc gcacccgtgc
    21781 gcacgaccaa agatcaccgg gcacgcagta ttttcgacgc ggagtcgtat cactacaacg
    21841 agaacatcat gacgctcagt ttgaaactga tgcgagtagt tcagaccgac agcgcgtggt
    21901 cggagaatcc gttgtcgaga acacaccgca tcgtccacaa ggtgcgggta tacgaaacgg
    21961 ataaccccga cgagtaccag gtttacagct cgatcgttct actgcgctca cgcaacgacg
    22021 aacctggcct cgagatcctg ccggctcgcc gactggacac gatccggacc ggcgactgga
    22081 aactgacctc gcgcacagtc tatttcgatc aaaccacgct gagcgtgcga aacctggcgg
    22141 tctacctgta gcggtcgcgc gtccgaaaac gagaacgatg aaggtgagga aacttcatgt
    22201 catcgaagac cgccgagctc ccgggttatc tgcccggcga ttggcggatc gacccacagc
    22261 gttccacgtt gaagttctcc atcggtcatt tgggattgca taccgtggac ggcacgttgg
    22321 ccatctccgg tgagtttgtc atggcagacg agccggaggg ttccagcgtc tcagcggtca
    22381 ttgacctcgc gtcggtgcat accgggagcc acggccgcga taagtcggcc cgctcgtcgt
    22441 cactgctcga tgtgggcaac tatcccaccg ggcactaccg ctcgactgcg gtcgtgccag
    22501 ccgctcctgg cggcaatccg cggaccttcg tgctggaagg ggaactgacg ttgctcgatg
    22561 taacacggcc ggtggcgctg gatatcagcc tccaggaacc cgcctccacc gacggtcggc
    22621 cgtgtcccgt catcgccgga cgggggcggt tcgctcgccg tgacttcggc cttgtgtatc
    22681 gcatacgtcc ccagttcctc gatcgcgcga tgggctcgac gatcgacgtc gagttgacca
    22741 tgcagggact cccagcgcct ccgagaggac ctcagccctg aagatcgcac gaacacgcac
    22801 tcatcaaggc gacgactgct ggtccctggt tgatgtcgct gccggcaccg tgcagccaat
    22861 ccgaggcgaa gtgtcgcagt ggggtccagc ggccgcagaa ggatgcacgg acttcgaaac
    22921 tgctggcgcg cccatgtcct tggaggatgt ccggctgcta ccgcccatca cccccacatc
    22981 cacgatcgtc ggggtgggga tgaactactg gtctcacctg gagaagctgg gcgtcactga
    23041 acggccttcc agcaccctcg gattcctcaa accacaggtg gcgatcatcg gccacaacga
    23101 cgagatcgcc aatcctttca tcaccgacca actcgacttc gaagttgagc tggtggtggt
    23161 gctcgcacag ccgatcaggg aggtcagcag caacctgact gactatgtgc tcggctacac
    23221 ggtcggcaac gatgtcagcg ctcgcgacgc cgcgccggcc cccatgggcg gactcgatct
    23281 gttctcggtc aagacgctca ccggtaccac tccggtgggg ccgtggatca ccacgaagga
    23341 cgaatttggc gggtcaggag aggtggatgt caccatctcg ttgcgggtga atagcgaaga
    23401 acgccaacgc gaccgcaccg gcaagatgat cttcggcatc gaggagtgcc tggagtacat
    23461 cgtcgccaga atggcgctca acccgggcga catcgtgttc accggcacca ccgacggcgt
    23521 cggcttcgag gatggacgct atctgcaggc aggcgatgtg gttgaggccg aagtagaagg
    23581 agtgggtgtg cttcgcaatg tcgtcggcgc aaaacaatca ccgacacgct gagcccggtc
    23641 agcccgctga cgccacggtg ctaagcccgc tacgcggtga tggtgccgct gtcgggctcc
    23701 acagccagat gagccttgag acatcccggt ggcgcgcacc gtttgtgcac atgtcggtgc
    23761 cggggtcggt ggagatcatc cgatccggcg cgaccaccgg cctgttcggc tattacccgg
    23821 ttaccagtcc atagcggtat cggccgaggt cgggcttgag cgaaactgtg tcgacggctg
    23881 ttagtcccct ccggttgtca ctgctcccca gctagaccgg tctaggaagt gcgccgtgac
    23941 gaggcgaact ggtcgatgaa ggagcgcacg ccgcggggga agttgccgcg ccgtaccgca
    24001 tcccagtgca acgagcggtc gaactcaagc gcctcatata acggcaattc agcgccgccc
    24061 agtactgcgc gcttcgccgc ggccaccccg atcctgcccg ctttcgccga taccgccgag
    24121 gcgaaagacg ttgactcgga caacaattta tctgccggca cgactctgtc gactagtcca
    24181 actgaatatg cctcgcgcgg tgaaatcggt tttgcatcaa gtatgaactg cagcgcgcga
    24241 ctgggctgca tcagtcggtt caggaattgg acaccaccgc cgccactggg taatgcaccg
    24301 aacagatcca tctccgggac gccgatcttg agcgtgccgg tgtctgccac gtagcgcagg
    24361 tcgcacgcgg ctgcaagctc cagcccccca gcgagcgtgt gaccgttgag ggcggccagg
    24421 aaaaggatcg gactgcggcg catcgccacg cacaacgcgc ggaaccgatc catgctcttc
    24481 gtgaattctg cggtgagtcc ctcgaccccc agcctgtccg cttcgccagc catccatgtg
    24541 gcgtcggctc cggcggagaa caccttgaga ccgctggtga tcacggttac cgcaacggat
    24601 tcgtcagcat tggcagcgtt gagttgacgc tccaacgctt cgagcagcgc cgggctcaac
    24661 gcgttcacgg gggcattgca gagcctccac agctggtagc cgtcggcctg cgtgacctcg
    24721 aggagggcgc tgtccttagt ggcgggctgt cgggtcgggg tcaattacgt cgcctccttg
    24781 aagtgagcgt ggtttctgat cgggggtcga tggcgcgggc gcggccccat cagcggccca
    24841 tgtttggagg accttgcccc gaatctttcc gacaggatcg cggggcagct ctgtgacgaa
    24901 gtggaatcgg cgcggcgcct tgtaggggga gaggcggttt cggacatgcg cctgtaattc
    24961 cgttgcaagt tcatcgttgg tgggcacccc tgggcgtacc gcgatgaagg ccacgacgat
    25021 ctcctgccgt atcgggtcgg gcgcgccaac gacggcgact tctcgaacgg cggtatgggt
    25081 gcttagtacg ttttcgactt ctaccggtgc caccttgtat cccgaggtgc tgatcaagta
    25141 atcggtgcgc ccgagatagg ccgcattgcc cgtgccgtcg aactcgatga ggtcatcgca
    25201 tattgaccag ccgtcaacca cgtcacgtcg ctgcagttcc ggacgattcc aataggtcaa
    25261 gccagtcagt ccccgcacag ccatctgccc gcgacccgtc tcgatgacct gcagccctga
    25321 gcctgtgcgc tggaccgcgg cgacgatgta gccgggcagc ggacgtccca gggaggcggc
    25381 cgggaaatct tcgtcatgcc ggggagtcat cggccatgtc gcgaatgcgg tgctgccgta
    25441 gttgttgatc aatgtgatac cccgcttttg ccagcgcata cgcacatctg ctgacgacgc
    25501 ggactgccac atcgcgtatg cacgtcgcag cgagggtagg tcgcgaccat tttcgtcgag
    25561 gacgtcgatc atgcgggccc acgttgcggt gatcgcagta aacacgttga cctggaagtc
    25621 agcgatcgcg gccacgaccc gctgaggatc ccgaaaattt tcgatcagaa cagttgtcga
    25681 accgtgcagg agggtgccgg tggtgtggta catgaagccg agtgcgtggc cagccggcgc
    25741 cgcggcggcc caccgttgac cccgagccgc cgcggtgacg gcgcctaagg cgtagccgcc
    25801 gaggaggaac ctgcgatgcg tgtggtagca gcccttgggt tggccggtag tgccaccggt
    25861 gtgccagagg accgcgacgg cgtcgctcgg cgtcgtcggt aggggcgcgg catggatctc
    25921 cgtcgcgtcg gtcaggatgc gcagatcacg gtagggtcca gcaccgctag gcgtgtcgcc
    25981 gtaggtgaaa acctcctcaa tcggtgagcc ggcgaccgcc tcggcaacga ctgcgaggtc
    26041 atcgccggtc gcagcaacga caaccagaaa acgggccgag gtgtcgtcga ggaagaaccg
    26101 caactcttcg ggttgcgctt gcaatggcgt cggaacgaca acccctccgg ccttccagac
    26161 ggctagcatt ccgatgatca gctcgggtac gttgggcccc cgcagggcga tcctgtcacc
    26221 cacgaccacc cccagcgcca ttagcccttg tgcgagcaaa gtgctaactt ctgcgatggc
    26281 actgaaggtg tatgagcggg ccgtatcggc gtgaatcatt gcgaggtcat cgccgtggcc
    26341 ggcgcataca tgagcatcgc tgagcgcgta accgacgttc gcccaagcgc ccactaagtc
    26401 gtggttgggt tcggtgtagt cgggttgatc ctgcggagcc acgaggcagt gagccggaac
    26461 gcttgtcggc ggatagcgat ctttcattaa cgtgtcctgg tgctggtgtc caggcgggca
    26521 gcggtgtttt ctgccggttg cagaagcgca ctgacgtcgg cagtgatttg atcaagagtg
    26581 ccggccaccg cggccaacac gtaacggtcc ggccgcacca cggcgacatc ggcaccttgg
    26641 ctgtcaagcc actccgccag tgccgggtcg ggctgcggca ggtcggcgat cgtggtcacc
    26701 cacgcgccga caacgtcggc ccaccatcga gacgtcgatc ccaacgcctc atcgtgtcgg
    26761 cccagaacga gaaaccgctg gccgaccgcg tcgtcgagcg gtttaccgcc gacgcgaggc
    26821 tgcccgaaca ggccgccccc tccttcgagg accatgggac cgggttgaag cgacggcagc
    26881 ccaaagaaca gcgagcgatg gtcgcgccgg atcgcctcgt cccgctcgcg cgctgcttgc
    26941 ggatccgtga tcccgacgat ggcgccgtag tcgatcgccg ccttgatgac ttcgcgcaca
    27001 tgcggtgacc gttcgatttc ataggtgtcg aggagatcta ttggcgctcc gcgcaatacc
    27061 cggtcgatct tccacgccag attggtcgca tcgcgcaaac ctgaacacat gccctgacca
    27121 aggaagggcg gcatctggtg cgcggcgtca cccgctagca gaagacgcga tgagcgccag
    27181 cgttgtgcca ccagtccgtg gaaggtgtag acagccgcgc gttccaccgt cccgaagtct
    27241 tcgccgacga gttcgcgaag caccgaggcc actgcggtgg gttcggtgat ggcagcggtg
    27301 tcctcgccgg aatgcaccat gaattccacg cggtgacgcc gacctggcat cgggatgaac
    27361 acatgcgggc gcgcgggatc acagacatag agggcgtgtc cgggcagcgc cctgtccgcg
    27421 tcgcccagta gcaggtcgac caccagccag cgttcgtgga accccagatc ctccagccgc
    27481 attgccgcca tttcccgaac ggggcttgcc gcgccatcgc aggccaccgc gtagcgggcc
    27541 cgcaccgtac ctaccggttc ggactcttga ttcccacctc gccatgcggt ttgcaggctg
    27601 acctcgtttg tgtcttgggt gacgtcgacg acttcggcct caagatgaac tgtgacggtc
    27661 ggcaacgctg cgaccgcggc gcgtaacgcg cgatcgaagc ccggttgatg gaaatacacg
    27721 ctggtaggaa cgcccgacca ggtggtctga ccggctggca tccacatcag gcggctgcgg
    27781 tcagcgctga cgaattcgat gcctgggttg agtgtcatcg aaggcagcac ctgttccagg
    27841 cagcccaatt cttggacggc tcgcaaaccg gtgtggtcga tgtgcgccgc ccgaggcagc
    27901 gcaaacacgt cggcatcctg ctcgagcacg atgacgtcca ggccgcggcg gcctaacagg
    27961 ccggcgagcg ccgttcccac cgggcccatc ccgatgattg ccacgtcatg gatagctgtg
    28021 cgggcatccc cgtcacgaag tcctccgccg aagaggcgtg ccatgccgtt ccttccgtcg
    28081 tcagtcatct gccgccgtgc gacccagcac ggtgacagcg acggccgcag tgtcacctcc
    28141 ggcatatccg ccggcgttct ccgccacgcc tatttgcgca tcgggcacct ggcgcccacc
    28201 ggcctccccc cgaagttggt gggcgagttc gatgacctgc gcaagaccgg tggccgccac
    28261 cggatgtccg cgcgactcca gaccgccgct ggtgttgacg ggcagctgtc cacccagaga
    28321 agtcagtccg tcggcgacta ggcgcgcgcc ggcgcccgga tcgcaaaatc caagttcctc
    28381 gtaggcaacc agttcgttga aggcgatgga atcgtgaacc tcggcgacgc tcacctggtg
    28441 gggtgtgatg cctgcgtcgg catatgcacg tcgggccacc ccgccgatcg ttgacccttg
    28501 gcgacccgcg gccgccatgc cgactgcgga gccccggatc cacaccggtc tgtgctcggg
    28561 tcgcacggaa accccggcag tgaccacaac cgccgttgcg ccatcgctga tcggtgcgca
    28621 catcagcgcg gtgatcggtt cgacgacgga ccgcgccgcc aagacttcct tggccgtcag
    28681 cggctgcctg tactgcgcca tcgggttgag ccccgcatgg ctgcggttct tgacggcgac
    28741 cgcggcaagg gtatcggcac tgaggccgtg ccggtcggcg tactcccgca gccgcggagg
    28801 gtagacggca gccacaaata cgctctcgcg ggtagcgtcg actcccgatt cggcgacaaa
    28861 gtcgatgtcg gcggcgccgt tgagcgccgc gtaggtggtt tcgcggctgc gcgcgaaaag
    28921 tttctccaca cccaacacca gcacgcgtgt ggccgcgccc gcacgaacgg cgtgtacggc
    28981 cagattgagg gcactcgatg accccgcaca cgcgttgtcg atgttgtaca cagggatacc
    29041 ggagaacccg ttgtcgcgca gcacagtttg gcccacgatg ctcacttgcc ccgtgacgat
    29101 ggaggccatg gcgttcgcga cgaaggccat gtcgatatcg gcgggggaca tggacgcatc
    29161 ggccagcgcc gcgtgcacag cctccccgcc aagacccttt agcgaacgtt gggggaaccg
    29221 gccaaacggg gtcatcccga ccccgatgat cgccgctgcg gtggtcatgg tcgatcagac
    29281 cgggtcagcg tgtcttcgaa accgacgatg gtgtcgtgca gcacgtgtcg ttgatcgagc
    29341 acgactaggt gtccgcattc gggaagtact cgcaggcttc cgcgtggtgc ggactcggcg
    29401 catgcctgcg cctcccggat agggatgacg tcgtcgtctt ggccgtgcac gaacagcacc
    29461 ggcttgctga ccgattcgca cagcggcagg ggatcgaact cgcccagcag cgccacccct
    29521 tgcagcgccg aacgtagggg cgtcgcgccg aatacccgtt ccagccaact cagcgttgca
    29581 tcgctttgtg gttgtttgca gagcacggcc gccgagcgcc gcgcgaactc gggccagtcg
    29641 cggcgcatgg cgcgtgccat ccgttcggca ggaaacactt tgcaactggg cacaccaaca
    29701 gcgatgactc cggaaacccg ggagtcatcg cgtgcagcaa cggtaagcgc aaccgcggcg
    29761 ccgaaggcaa agccgatcac aaggacgtcg cgtaagccct gctcgaccag cagttctatc
    29821 accgcatccg tgtgcgcgtt catatcgtag ggctcaggca tcgtgtccga acgcccgaag
    29881 cccggcaagt ccggtacgaa tacctggtgg tcgccgaaaa gctcgtcttc ctcgtaggcc
    29941 cacagcgatc catctagaca ccaaccgtgg agcaacagca caggtttacc gacaccccgc
    30001 ttgacgaacg acatacccga tgctgtcctc ttacgcgcgg acccgccgtt agcggcactc
    30061 atcgatacct cagtgccggc accgagtcga cccagctgcg atgttcgacg accggcaata
    30121 tccgcggcac accgcagggt ggtgattcct tgaccaggac gcatttattc gtctctagtc
    30181 gtcgagacga tggtctctgc gggtacgacg ctgagcgttg aggaccggcg tgggaggtca
    30241 tcgagcgacc tcggtctgct gtggggaaag gcggatcatg agcccgatgt gtccaatctg
    30301 taccacctcg ccgcgttgat tggtgatgcg aaacaggcga tcgacgacgc ctcggtcacc
    30361 actgctcgtc aggcgcttgg acacaatctc caccgtggcg gacagggtgt ctccgacgaa
    30421 aacggccttc tcgaaccgcc actcggtgat atcgagcatc gctatcgccg agcctgagaa
    30481 gatcccgctg cggtccatca gaccggtcat catgatcaga ctgaggagtc catacaccac
    30541 gggttggccg tagatcgtgt ctgtcgcgaa ctcacggtca gtgtgaatgg gattccagtc
    30601 cccggagagc atcgcgaagc caacgaggtc actttcagtg accgtcctgg ccggcagctt
    30661 gaagacccgg ccgacggcaa gatcgtcgta gaagaacggc acggacagtt acattacatc
    30721 cgacgctgtt caacagccaa ctatacgacc gtcttgcaca ctcggtgaaa tcctgcaata
    30781 tgacggcacg accaacggtg aagtcgcgca gccggtgggc tggcgcggat gaggcgaccc
    30841 caattgggcg ctggggacga gatgccgcca gcggtcgtga gttggacacg gagtggggcg
    30901 gggcggggat accagttgtc aacgccgtga cagaggatct ggacccggca ttgacgcatg
    30961 agtgctgatg gcagctggga tgtgatgccg gcaaggttgc tggccactgc cgaggcggct
    31021 ctggtcatgg agcccttcgc ccacgtacag cacggtgtgt caaacgatgg attagtcgga
    31081 acgttggcga aacgtggaag tgtcgactgt tttaagcgca gccgccagcc tgggcgccgc
    31141 gagacaactc gagtcggcgc ccggtgggcg actcaacctc gctgcctgcc cgccggcctg
    31201 attgcgcttg cgttatgagg tgttctgtcg gtcttgtcta ctgggcggca tggctttccc
    31261 gggcggtgcg acggcgctac atatagacaa cggctcaccg gtgtcaaatg taatattgct
    31321 gtgctgacca gcgggcgcac tcggtctaaa agcattaatc cgttcggtga catgacgtta
    31381 atcggtcacc atcaccacac ggcccgacca accacctggt cccaggtcat gacaaaacgc
    31441 gtgtcgttgc ggagttggca cctatcgcac tggtcagctc ccattcggcg ggaccagaag
    31501 gtcttgccac ttttggtggt tggcaccatg agtgaggtca gtctgcgtgt actgcttgcc
    31561 atctggtccg gtgtaggtgc cggtgttggg gtcatattcg acaatggcga ttggcggtgg
    31621 cgccggggac gcggccggag gtgtttctgg cagaggctgg gggatgggct ggccggacag
    31681 tgtcgcgttg ggatcgcctt tccagttatt gccgtcgttc aacggtacgt actgttcgtc
    31741 gctctcgcac atctggactg tgggggccct cttacccggt cgcgtcatgc acggaatgtt
    31801 gcgggcaccg cgaacattaa gcgatgaatc ctggggtact cggcagtaca gcatgccggc
    31861 cgggcgttcg ggatagtcca cgtcgctcgg agggcgcatc tgctgagccg gcagaaaccc
    31921 ggtggtgcat ggcggaggca ggttggcgtt cagattgagc gacaggtaaa tccctttgtg
    31981 atctgtgttg cggtcggcga ctagaggcgc ttgcatttct tctacatttt ttgggagtag
    32041 cacgaggagt tgctcaagac tcggttggta tgtgatggcc acctgctcga ggctgaccaa
    32101 gttggccagc acaaccggta atgttggctg cagcctgtcc agcaggttgc gcacttcgtc
    32161 cccggcggcg gggccattgt tcagtagtcc gttcaacgcc gggttctggt cccgcagctg
    32221 gctggttatc tgagcaaggt tggcggccca ggcttgaatc gagtcggaag tgtcagtctg
    32281 agtgtccagg atgggtttcg attgatcaat caccgtggtg atcgcactca gattggcgcg
    32341 tgcgtcagcg gccagtgtcg ttgtcccgtc aacgattctc ttgagctcag gtccgagacc
    32401 ccccactgcg gtgtaggcct cgtcgaccgc cgtattcaag tcatcgtgtg gaatggcctg
    32461 aaggccgctg ttggtgtcgg ccagcactgc gttgatgtcg ggaggcaacg ccgtgcggtc
    32521 gaccggaatg acgtcgccgt tcttcagcga aggtccgtca ccgctgcgtg ggttgagttc
    32581 tagagatagt tcgccgaccg cggtttggct gcgcacctga gcgacgaggt cggccggaat
    32641 tttggtgtcg gaatttaggg agaggacagc ctcaactccg ttggggccca accggacgtc
    32701 ctgaacgcgg cctaccgcga ctccgcggta ggtgacgttg ccgtttctat ataagccacc
    32761 agatgcgatc aggtttacgg tgacctcgta atgcccgatg ccgaagaaca ccgaaggcag
    32821 atctaggtat ttgaaggcca tggtgccacc agcgattagc gtgacgatcg taaagaatgt
    32881 caactgcagt ttgacttgcc ggctcaggta cacgtcacgg tccctggtcg taacggtagg
    32941 gggcgatcaa cgggttgcca agcgtgtagg ggctgggtag ttggccgata gtgcgtcccc
    33001 actgcatttc caacttcgta agtgtgcctt cgaacctggt accggttaag aaggacgagt
    33061 cgagtcggct gagtgtcagg tcgacgatga ggctgatgtt ggcgtagtca ccgcggaacc
    33121 attttgtcag tgtgtccttg gtgaatggga atgttgggag aaagttcagc gaacgagtca
    33181 acgatctgcc tgcgtcggcc agcgattgca gcacgggccc aaggtctttg agttcctgaa
    33241 cgagcgcatc cttggtctgt tggaccgaat cttcagcaag agcgctcaac ttgccaagct
    33301 gatcaatcgc ttcgataagg ttgtcttgtt cgcgactgag gatggccagc gcgtcaggaa
    33361 tggtgcgcag cccttcatcg atcacgggtt tttgctcggc gaattgaccg accaatctat
    33421 tgaaactatc ggtggccccg atgatttcgc tggtttggtc attgagctgg ctgacgaaat
    33481 tgtctatttg agacagcagg ctgcgcaggt caccctcacg gccggcgaac gccgtgctga
    33541 gcgcttcagt gatgtcctgg atctggccga gcccaccatt attgagcaga agtgaagccg
    33601 cggccagcgt ctgctcagtg gacgggtaag ccttgcccga cgacagaggc agcaccgacc
    33661 cctgatgaag cttgcctcgg gacatgccat cggacggcgg cgccagctcg atgtgaagcg
    33721 agcccaggag gctcgtctgg ccgagggtcg cggtcgagtt ggcgggcagg ctgatgtcac
    33781 cattgagtct gatcgtcacc agtgcatgcc agtcttggcg ttcgatcctg gtgatggtgc
    33841 cgacgtcgac atcgccgacc cggacgcgct ggttgggctg taggttgctc acctcgggca
    33901 tctgcgcctg gattgtgaac gctccggggc caccgcccgc acttcccggc aacggcaagc
    33961 tattgaggcc ccgccacccg cagcccgaaa gtagcgtgac cgccaccgcg ccgaccagca
    34021 cgacggcggt cattcgtcgg gatgctggtc gcatcacgat cctgcgcccg gcacaaccat
    34081 gatgcctggc aggcctgcgg ccggatctgt ttggtgcggc tgtgccgact cggctgccag
    34141 cggcgcctcg ggcggaagcg ctgagaggcc agccggcgcg gggggcgcgg ctggcggcgt
    34201 tggttgctgc ggaacgtagt cgggtcgcat ccaatcctcg ctgtaggtga gttcattggg
    34261 ccgcgcattt gccccgacga agggattgaa ccccaatgga gggaagttgt actgccggtt
    34321 cttgacgatc ggagcgagat actgcacgca caatttcgcg gattgttctg cgcccaaacg
    34381 ggaggcggct tgtatcgcgc cgcacaggaa ggtgatgggg ttagcgaagt tgttgagaac
    34441 gggcgcccca ctgagcgtgc cctgcgcggg ctggtaaatg ttggtggcgt tagctgcgag
    34501 tgtcggcagg acatgcagca gttgtttgac gtcatcgagg ctctgggtca gcgcggatga
    34561 gaccgatgcc agcttgtctg aggttgtgcc gagagcttcg cggttgtcgg tgacaaagct
    34621 tgttacttcg ctcaccacat cgttaaggtc tttgactgcg gtcgccacct cgccggggtc
    34681 atctgcaagc aaacctgtca ccgatgccag gttcccgttc agctgtctga gcaggtcggt
    34741 gctgtcctgc agtgctgaga ccagaaccga caagtttttg acagtgctga agatgtcgtc
    34801 gctatggtcg ccgagtgtcg aaatggcttg tgaaagcttg attatggtgt cgcggatggc
    34861 ggcaccttgc ccgcgtaggt tatctgcggt ggtgtggatg aacgagccta gagtgctcac
    34921 cccaccggct ttggtgggtt gcagcgtatt ggcaagccgc tgcagctgga ggcgcacgtc
    34981 gtcccattcc actggcacag cggtgcgctg ctttgagatg accgcgttgc tttgaagggt
    35041 aggtccatca cggtagggcg gggtcagctg gatcgctcgc gccgtcacta gcgtcgggga
    35101 caggatcacc gccttcgcgt cggcggggac cttgtatttg ctgtcaaacc agaacgtgat
    35161 cttaacctgt gtgggttgag cttcgatttt tccgatcttg ccgacgggaa cgccgcgaat
    35221 ccgcacgtca tcgccgggaa ataatccgat gctgttgtcg aagtacgcgg ttacctcgat
    35281 ccggtcggtt cgctcgcctg accaaacgag gaaatatccg gcgccggcag cgatgaagac
    35341 gaggacaatg gccatggcaa tcctggcgtt gcgcacgctg atcattgccc ggcgccttct
    35401 gcgggtgggg ttgtgggggc tagttcgccg ggcgttggaa cgagcaccgc gctggggttc
    35461 ggcggagtcg acgcgttcgg atcgggggct tgctgcgcgg gcggccccgg tggcggcccg
    35521 cccggcgcgg gtgccggcag cggctctcga tacggatagc ccgggtcccc aggatttccc
    35581 gtgatcgcct cgggcagcga aagattcggc tcgcccccct ggccggtacg agggtaggga
    35641 acgggcagtg ccggtgtgcc cggctggccg gtcggaggat cgacgcgctg cgacggcaac
    35701 agtacattgg ggtctaaccc caagtcggaa aatgccgcgt cgacgaaggg ctgaaggaac
    35761 tggcccggga ggagattcgc cacgtagacc ttgaagaagg ggcctccaga tagcgattca
    35821 ccaaggctca acgcatacga gttcagaccc ttgatggact gttgaatctg gtctttacgg
    35881 ttgtcgacgg tcgccaatac gccgttcagc ttgtccagcg ccggtttaag cgtcgcccgg
    35941 ttgtcagaaa tgaatccttg aagctgctga gccagcgctg agacattgcc cgaaatgtta
    36001 tccagcgcgg cgctttgacc ttgcagggcg gccagcacgg cattggtgtc gtgaagcagc
    36061 tggacgattt catcggtgcg ctccgccaga actgtggttg ctttactggc gttcgacagt
    36121 aggttgcgca actcggcgtc gcgccgatcg atcgtctggg agaagcgggc gacgccatcg
    36181 actgcaaccc tgagttgcgg cggcgtgtcc tgcagggtgt cagacaaggt ttgcagtgcg
    36241 tcggacagct gagtcgtgtt gagtccgctg atggtcgcag tgaggtcacc gagcgcatca
    36301 gggagctgaa atggcgaggt ggtccgctcg accgggatgg cggcactcaa gtgtcccgaa
    36361 ccgcgaggag tcacttcgaa aaccttggtt ccgagtaggc ttttcgactt gatcgccgcc
    36421 tcggtgcgct cgcccaaccg gatgttgtcg gccactgtga aagtgatcag caccccgcgg
    36481 ggagtcagcg agatgtcttt tacctggccg ctccgcaaac ccgacacctg caccggtgca
    36541 cctgtagaca gatcacccac ctcttcgaag tacgccgagt aggtgctgcc actcgaaatg
    36601 aacggcaaat tgctgtaatt cagcgcgccc accgtggtgg caccgaccac ggcaagcgaa
    36661 atagcaccga cgattatcgg gttgcgttcc cgaaatgatt tcatttggga gtacacctgc
    36721 ccgagtcttg gccgactaac ttgacgtaga ccggttggcc gcccttgccg ttgactttca
    36781 gcgagatgtc gcacaagtag aaattaaaga agtcgccggt cagtgcctgg cggcccagca
    36841 cgcggtacgc gtcgggcagg gtattgagaa aatcgtcgag gtaatcatga tcggccagca
    36901 cgacggcggc agtgcggtca gcctcttgga cggttttctg tagcgctggc cgagcttggg
    36961 ccagcaagtc agcgatggtg gcagcccccg cgttcgcgga cgccacggcg ccgctgatgt
    37021 cctggctgcg gccttggagc ccgtggacca ggtgcgagag cgagtccacc gccacgccga
    37081 gcttgtcgct gccaagacca agggagccca gcacagtgtt cgcgttgatc acgacctgtc
    37141 cgatgagcgc gtcgcggtcg gctagtgtgt tcgtcagggc cgcagcctgt tccaggatcg
    37201 atccgatggt ggggccttgg ccctggaaac ccgcaatgag ttggccggtc agcttgttga
    37261 cctgatctgg atccaagccc cgaagcagcg gtcggaatcc gccgatcaaa gcgtccaggt
    37321 caagtgcggg cgcggtgcgc tcgagcggaa tcgttgcccc gggcggcagg ggcatggtaa
    37381 cccctgggcc ttgttccaac gccaagtagc ggccgccgat gaggtcgtta tagcggatta
    37441 cagctcggct gcccccggtt agtaccaccg aatcatctac gctgaatttc accagcgccg
    37501 aagcatcatc actgatggtg atgcccttga ccttgccaac ctcgacgccg gcgatgcgaa
    37561 cgaagtttcc attttcgagg ccgctcaccg tactgaagat ggcgttgtaa cttttttcct
    37621 gattaaaccg cacctgcgca taaatggtga tcagtgcggc agcgccgagc acacatacgg
    37681 acaggaatgc cgcgagccgc cataggagcc ctgttaggtt acgccgcaag gcaatcacct
    37741 ctcgcgaatg tcgtttgtgg tcggttctga gtggccgagg tgtcagggtg gcgctggcgc
    37801 gcccggatct gtcgacgcaa cagctgactg gtttgatgcg ggcgtctcgg gggatggagg
    37861 aggcgctggc acccccgggt agagcggggc tccgtcgggc ccatattgcg gtgcgccgta
    37921 tggcggggcg ccggggtagg ggatcgggcc gggtgcgggt ccgccgggat agcggatgct
    37981 cggcggctcg ggcactgcgc gggtcactgg caaatagttc gcgtatccgg ggaagccgat
    38041 gcccgggttc ggccgcatat ccaggccggt gccgaaacct gtgtccgtta cgagatagcg
    38101 tacgggccag ttttgggcaa cgtcgggcag cgatccgcac ccaggtttgc ccccgggtcc
    38161 gcccttggcg gcgacgattg gcagattgtc tggatagcga tacgggtcat cagccaatag
    38221 caccgtggcg tccatgacga ccgacttgcc gttggaaccg ccatacaccg gggacagctc
    38281 ggcgccagtc ctatcggcgc caacgagaag acaggtgagc tcggggttgt acttcatcaa
    38341 caggctcgtt gtgggctcga gcaggttgat tgttttgacg aaattctcct tgtttgggcg
    38401 tacgagctcg atcccgcgtt gggaaaggcc ggcaacgctc accagtagct tgtccaactg
    38461 tgcagcttgc gccgttaccg tggcggccgt ggtactggcc gccgctacgg tgtcgatgat
    38521 gttttgtgcc gcgtcgctgt aggtgtcact gacgttcgca agagcctgaa agtccgcgcg
    38581 caaggtgtca gtgcgcgagt tgagtgccag gagaacgtca ttggactcgg taatggactg
    38641 accaatctgc tctcctttac ctcgtacccc accggctagc gcagagagca cagcgttgag
    38701 cttgtacggg tcgacttgct ttatcagctc caccaagttc tggaacactg tgttgacttc
    38761 gatagcgacg ttttctgatt tcaggacggc ccctgccgat agccgctggg gactcggatc
    38821 cgttggtgga atcagatcga cgtatttcgc gccgaaaagc gatgtggcgt tgatgcgggc
    38881 cttgacgttc gcggggatgt tctcgatctg gccgggatcg atgtcgagct caagagtcgc
    38941 cgagtcggcg gtgcttgcga ctgtctcgac ccggccgatc tgcaccccgc gcatcttgac
    39001 cttggcgtag ggctccatta ccaatccgga acgatccgca gttaacgtca ccgccacacc
    39061 tgggctgaag gaccgattga acgcgcccag actcaacgcg gtagcgccga acacgccgag
    39121 gaccaacagg agggcccacc aactagggtc gatccgcggg aggcgtggtc gcttttgcat
    39181 cggcctatcc cgcgaagttg aagttgccgg tttgaccgta gagcgccagc gtgacgagca
    39241 tgatgacgaa cgccgaaacg atcagtgagg cacgcgtggc tcgccccacc gcttcgccga
    39301 cgcctgcagg accgccgctg gcggtgaatc cgtaataggt gtggaccagc attatggacg
    39361 tcacctgggc cagcgtgacg atgaacgacc gcaccgcatc ggcgggaatg aaaaacgtgc
    39421 tgaagtagtg cccgtagacg ccactggact gcccgtagag ggcggtggtc acgaactgac
    39481 cggacgtata tcccagcagc agggctacgc agaatagtgg gacagcaaca atcacaccgg
    39541 cggctacgcg ggtcgatgcc aggtaggcaa tggaccggat gcccatgact tcaagcgcgt
    39601 cgatttcctc gttgatccgc atcgcgccga gctgcgcggt ggtgcccgca ccgatcgtcg
    39661 cggcgaatgc gaaggctgcg gtacccggcg tcacgaggcg cgtacttgcg aacgccgaaa
    39721 cgaatccggt caccgcgtcc actccgactt cagaaagctg gttgtagcct tggctggaca
    39781 acacgccgct ggtggacatg gtcagaaatg ccatcacagc gacggtgcct ccgatcacgg
    39841 cgagtgatcc ggtcccagca ctcattcctg cgatctggcg aagtagctcg gctcggtagt
    39901 ggataacgac gtcggcgatt gagccgagca ccttgccgta gaaccgcgtc tgctcaccga
    39961 ggcgtcgcca ctccgcccta gcagtgccca gcatccgtag tgccgccacg gggacacgtt
    40021 ggtgggcaag ggtgctggcg ctcacttagt cacctcgaac ccgatggcgg tgatgacaag
    40081 gttgaccatg aacagcaaga cgaaggagta cacgacggtc tggttgaccg cattgcccac
    40141 gccggcgggt cccccgctga ctgtcgtccc ctggtaaacg gcgatcaacc ccgccgctag
    40201 gccgaagacg gttgccttga ccattgacac caacacgtcc gacaggtcag tgagcagcgt
    40261 catgcccgcg acaaatgcgc cgggggtgac gtgttgaaag tacacaccga acaggaagcc
    40321 gccgacgagg cccgtcatcg tcaccaggga ggagatcatt agcgaaacga gcgtgactgc
    40381 cagcaccctc ggcgctagaa cggtgcgtac ggggtcgata cccatgacgc gcagggcgtc
    40441 aagctcctca cgaatcgtgc gcgctcctag gtcggcgcat acggcggtgg cacccgatcc
    40501 ggcgacaacc aggacggtta cgaagggccc aatctggttg accgcactga ttgaggcgcc
    40561 cgtgccggat aggtcgccgg cgccgaattc aatcagcagc acgttgaagg tgaagaccga
    40621 caggaccaca aacgggattg ctaaaacgat cgacggtaaa aatgacacgc gcgccacgaa
    40681 ccacgtctgc tcgaggaatt cacgccacgc gaaaggccat ttgaccattg cgacgaaaat
    40741 gtcgagtgcc gtcgcggaga agtcacctaa ggcgcgcacc ggcgctagtg ccggcctttc
    40801 atacccgacc atcagccacc tcggcgtcgc ctgatcccgt gtcgacgaaa atgttgtccc
    40861 cgtcgatgac gaccggaaat gttgctaccg gctcagttgg tgggggtgcc accggcgcac
    40921 cgcttcgcac atcgaaccgg cctaagtgcc acggacactc gacctcgcac cccactagca
    40981 cgccctcgct aagagtctcg gcttcatgcg agcatcgatc ctcgatggcg aaaatggtgc
    41041 cgtcgtcaag ccgcgcgagg cacaaagcgc gaccagctac ctcgatccgg cgcatctcgc
    41101 caatgcttag ctcatcaagt acggcaactc gccgtaacgt cgtccgatcg ggcgtctccg
    41161 gcgatgatgc gcccgaaccc gatgaggtta ggtccgccac aagtcatggc tcccggaact
    41221 tgccggacgg tctacaggta tgtacccacc ggtgccgacc gctaccacaa aagggttaag
    41281 gctcaggcgg gcttttctcg cggcggggtg agaacgtacc ggcgctgtcc gtgggcctcc
    41341 tgttaggccc cgctgcccaa ccgtttcccg cgccatgcgg catattcaat acttcaccgg
    41401 agtcgtatgt caaccttatg atgacagcat cgacggacgg agcgctctcc gtcccaaagc
    41461 ccccggccgc ggggagcccg catgactggc ctgtgctcga ggactggcca ctttctaagg
    41521 aggcaacagt gggtaggttg atgggcaaga ccgcagtcat ctccggggtg gcaagcggca
    41581 tcggggcgct cgcttggcgg aggttttgcg aggagggcgc gtcggtcgtc ggcatcgaca
    41641 tcaacgagtc agcgggcaag cagctggccg acgagcttcg tgcgaatgga cacgatggcg
    41701 aattcttcac cgccgatgtg acgtccaagg aagcgataga cagtcttgcg gcgttgatcc
    41761 gagatcggtt tggtggcttg gacatccttt acaacaacgc tggaatcaac ctcgcgagac
    41821 cactattgga aacgaccgag gcagagtggg acaacgtaca caacgtggat ctcaagagca
    41881 cgtttctcat gatgaaggct ttcgcgccac ttatgcagga ccgcccggcc tcgatcatca
    41941 ataccgcgtc gatgggcggc cttgttgcgt acgacaacat ggcggcctac tgcgcggcga
    42001 agggtgggct catcatgctc accaaggcag cggcgctcga gttggcaccg gcggtcaggg
    42061 ttaacgtcat ctgtcccacc ctcattgaca caccaatgtc ccgacggttc gtcggagacc
    42121 tacccttgga gtttgaccag gtgtggaagg agttcgaggg cggtcatccg ctgggtcgaa
    42181 taggcaagcc agaggacatc gttccgctcg cggtgtacct cgcaagtgac gagtcgctca
    42241 tgatgacagg ggtcgccctt cccgtggacg gaggctggtc aatccggtga ggaagtaatc
    42301 ctcatggcgt tcagcgatga ggacgacagg gccgagccga aaccgcgctc ccgcggcgtg
    42361 attcgttcac ggtggcatcg accctcagtg gaaccgacgc cccgccccac ggcacattgt
    42421 tcgacgtccg accaggcacc cctggtgcgg tgcagggcct cgatttcgtc tacaacccgg
    42481 agcacaaccc tcgacaccaa tagggtgtgc ggtcacccga gcgaataacc tgaacgacgg
    42541 aatcgaaccg catcgcctca tggaaatccg atctcgcaag cgacggatcg ccgaaagatg
    42601 gcccactgcg ccggattcgg cgcgagcgcc tggagtacgt tgcccatgtc atagggggtt
    42661 gtcgcaaact aatacgctga tcttttgtga ttgtcgcctc gggatcgcgc gagccgtgtg
    42721 gtcgccggcg cttgactagg tgcgcgcgac gcgcaatgtg gactgcggga acagctcttc
    42781 gacggtcaat cgccgcggta ccaagccctg ttcgtgcata tacctgagga acgcgtcgat
    42841 gacatgctgg ttcgcctctg cgccgtacgg ccaaaagtcg cgactgccga acaatcgctc
    42901 ggtggcctcc gcctcggcgg tcagccacgg cagggccgtc tttgctgtgg cgggctcgaa
    42961 caggtcatcc actgcgatct tcttggcggc ttcgaaggct ttggtcagcg actgcgcgag
    43021 ccatgggtgt gaggtcagga gctccgcacg caccaccacc gtgtgcatga tcgggaagat
    43081 cccggtgcgt tcgtagtaca tctgctcctc gcggcgatag tcgggaaaga gccgccgtac
    43141 gtatggggcg ccgtcggcga agccgtcggg ggcgtgagca gtgtaaacgg catcgagttc
    43201 gccccgcgcg agcatagcgg agagtgtctc gtcggatggg atcgtatcga gctggatggc
    43261 ctcgggtcgg cgcagcgcgc tttccaggcg tccaggttgg ttcagaccgc cctggaagta
    43321 gcgcacggaa tcgaccggta cgccgtagta gtcggcgagt atcccgcgga tccagactcc
    43381 agcggtttgc tggtactccg gcacgcccac cctcttgccg atgaggtcgt ccggggtgac
    43441 gatgccgctc tttgtgttca cgtagatacc ggagtgacga aacatccggg acggaaagac
    43501 aggaatggcg accatgtttc ggcgctcacg aaacagcgat ccgacgtacc aggcgaagct
    43561 catctcggac gcgtcgaact ctgcgttccg gaacatgcga tgaaagctct cgggcatctc
    43621 catgggaatg tagttgaggt ccacaccctc tacgcgaacc cgcccgtcgg caagtgcacg
    43681 cgttcggtcg taatcccagc aggccaaatc cagtgctagc ggcataaatc tcctttttgg
    43741 tttaggcaac ttattcacgc actgtcgtgc tcgatagtca ccaactttcc accacaagtt
    43801 tcgttcggta gagcggaagc accatgttga tccgaatatc cttgacatac tatccgggtg
    43861 aagcgtgaac tattggggtg agcgacgtcg ccaatcagcc gagtgagcaa cgggatgtcg
    43921 gccgacaggt tgacagcacg ggcgcagcgg cgccaagtga cgatagtgag cgagcgaact
    43981 tcgcgatggt aagcggattg ccgcaggctg atggcgagtc acctctgtca cgcccgtcgc
    44041 gggatgccgc tggagggcgg atcgacctac tggaggagga acctgacgtg gagacaccca
    44101 cgggctgccc gtatcaccgc cgtgatgaac caacccccga ctcgatcgcg acggtgtcgc
    44161 ggtatcgaga tgtcgaagag attttccggt cacccgcgat ggccccgttt ctgcacgacg
    44221 gtaccgagca gtttcggggt gggaccgtgc gcctcatcga cggatccacc catcggttac
    44281 ggcgccgaac gatgggccag ctgctgcgcg gccagggtga ggtccggttc cgcgaggaag
    44341 tgctcatgcc gacaatcagg cgcaaccttg cgcgggtgtt atctagcgcc acccacggac
    44401 gcccttccac tgacatggtg cagttcgccc gggtggcgtt cttccagctg gccgccgcgc
    44461 taattggttt ggacggggtc aacaccactg aggacgccga acagctgcgc cagttcgccg
    44521 agccgatcca agtagccatg cggtcctggt atatgacgga cgatcgcacc gccgtgatgg
    44581 ctcaggggat cgaagttcgt gaccagttcc gtgagcggta cttcaaaccg gcacttgaac
    44641 gccgcgtcgc gctcgtcgcg acggcaaaga ctgccgagca ggaggcggaa ttgccgaatg
    44701 acttcctcac cctggtggcc cgcggggtag atcccgactg gagcgtcaac cgtgacttgg
    44761 cgttgcgcga gacgataacc gacttcatca acgccggaac gttctccagc tcgttcacgc
    44821 tcgtacacgc acttgacgag tgcttaactt gggcggaacg ccaccccgac gaaacggatc
    44881 ggctgctcga cgagcaattc ctagccgcgg gcgtggccga ggcgctgagg ctgcacccga
    44941 ttgttccgct gtcctaccgc accgcatcgg aagcggttac gttgaagtca ggccgcacgt
    45001 tccatgccgg cgagcacgtc tgtctcgagg tcggactggc gaatcgcgac ccggacgtgt
    45061 tcggcgccga cgtcgactcc ttcattcccg gtcgaccggt gccgccgggg gtgtacccct
    45121 atggcgtgtc gttcggcacc ggtcgccaca tgtgttatgg gcagccggtg attctcggaa
    45181 ccggcggaac cacgggtagc catgtgcaga tcttgaaggc gctgttcggc gccggcgtcc
    45241 agcgtcaccc ctcgcggcca ccccggttga gtggtgactt tggtcgaatg aatgctttgt
    45301 gggagaccta cccaattgag cttgagccag tgtcggcggt ctacgccgag tcgtcaatgt
    45361 gacgcgctcg acgtcccgag gggtgcggtc ggtgttgacg gtcactgccg caaccggtgc
    45421 aagcagagat agcggtcgcg gctaaccgtg gccgttgacg atcggctcga ttgacggtgc
    45481 cggggaaatg ccaaccgaca cgacatatgt tgaggagttt ttggtgagtg aagcgcaggc
    45541 cagcaaaatc gcgatcttcg tcggtcatat ccagcagccc atcgtgggca tggccccgga
    45601 ggggtactta gaccggctgg ctgagacatt ggcggcggcg cgcgcggcgg gggtaagtgt
    45661 catctatgcc aagctgtcgt ttcgggcggg atatcttgag ctccccgacc atgacccgat
    45721 gcgggcgatg gtcgacgcgg acaacctctt cattggtggg ctgtcgagca ccatcgatga
    45781 agcggtcaag ccgctggagg gcgacattgt gatagatgca gtgcggccta gtgcgtttgc
    45841 ctacaccgag ctggagccca tcctgcgcag ccgcggcatc aattcgattg tgttgactgg
    45901 tatttcgacc ggcggagtcg tgatgggtac cttggtagat gcgcgtagcc gggacttcgc
    45961 agtcacggtg cttagcgacc tgtgcgtgga atctaacccc tcggggcaag tggcacttct
    46021 ggaggcattc acttacccat gggcggccac catcaccgac gcgtccacct gggtgacgga
    46081 gctcgccgaa tttaccggtg cggttgggta atgatcctca actctcgggc cggtcaagat
    46141 cgcgggatca atctcttaac agtcatgtcc agtcatatgt tgtccgttgg ttcggcatag
    46201 cttcgtcctc cttcccttgt gcgtctttga ccagcggtta tgtggatggc ccctcaaata
    46261 ctcatcggtt taaataggcc acccaatacg ggcattgcca ccaacatgtg tagagtacga
    46321 gccgagtcga tgtgaggatc gggggcgtaa gttgtcggtt gtagacggtg gccatggacc
    46381 ggctgaggcg ccattatcgc gcgtagcgcg ctaatgagag ccccggaatg agttccgaca
    46441 agccttccgc aacggtggcg aagatcagcc cgacgacatg ggaaccgacg ccattcacgt
    46501 cgtcagctgt tgcctttcga ccgcaggaat tggtcataag cctggtgggc gagtacgtcg
    46561 aacccggggg acgggtgtgg tcaggcggcc tcgtacagct attagattgc ctcggcttta
    46621 cgaccccggg ggcacgggtc gccctaaacc gtctagtgtc acgcgggctg tttgacgtcg
    46681 tcaaagatgg ccggaaggtc ttctacaccg cgaccgctcg gctggtcgag ctgctgaacg
    46741 aagggcatca acagacattt tggttccgct actcgcagaa gccttggcgc ggtgactgga
    46801 ctctcgtgtg gtacaccatc cccgaggaac agttgctgaa gcgccgtcgg ctgagtcgtc
    46861 ggttggtttt tctcgggttc ggcgcattgc aggacgggac ctggctggct ccgcacgacc
    46921 gatcggagga attgtggacc gccgttgagg atctacagct tcgcgattac gtcgtcgtag
    46981 ttgtgggcgc ggcgcctgca tgggcatcgc gccagacaat gattcggcgg gcttggaacg
    47041 ttgatgaggc ttccgcccgc tataccgcga tcgtcgaggg ctttcggcaa ttctccaacc
    47101 gccgcgtatt ggcgaccgta acacctgagc gcgcgttcat cgtgcgcacg catctcatcg
    47161 aggcgatccg tcaagttgcg ccacttgacc caaagctccc ggacgccgat acgggcttcc
    47221 gctggcatcg ccgcctggcg ttggaggtat tcaaagacgt cgatgccgcc ttacagccga
    47281 tggcctcaga gtttttcaac gagaagactc gtcctcattc ctagaggtgg acggattgat
    47341 cgaggaggcg gtcgaactca ggcgtcagcg cgatcgtcgg gccgccctgc aggcgttcgg
    47401 ctacggcacc gagtcggtcc agccgagtgt cggccaaatg ccgtaacagc gagatctccc
    47461 atagggaggc tcgatgctca actagtagaa cgtcgagaga tgtcttgagt ttcaggctgc
    47521 gactcgcaag agtcgggctt ggtcgatggt ttcgctgatg tgaatgtcaa tggtgtgcag
    47581 cgcctttcgc acggatggcc cggcgggcgg gcggtgagca gcaggcgtcg cggcggctgg
    47641 tcatgcagtt tggtgtagaa gaccgcaaac cgcaggccac ctcaggagcc ggtaacggtt
    47701 ccagttgggg atgcgggtga tgagcccgtt gaccccgcag ccggcccagg tcttagctgg
    47761 ccttgttcat ggtgtacggc gcacggctga gcagtccggt cgtcaaggtg cgcagggtct
    47821 tgttggtgat acgggtgacg gcgagaacgc tcgtgcatgg cgccggccga aacctgggtc
    47881 aacggtcgtc gcccgagggc ttagcttagg tttcctcagt cttgagccgg gggccgtctc
    47941 ctcagctagc ggcctttcga tgcggtctgg ctccggactt cttgggctca agtccggctg
    48001 atcgtccgag agtgaccagc aggtcgcgca ggtcaaggta gtcgcgggtc ggtatgaagt
    48061 aatgcgaaaa cgccatccgc acaataagtt ccactacctc gtctacggtt actgatccat
    48121 tccggatcgc ggcgctgtca gccagaacag gctcaagagc gttgtgaagt accgcagaca
    48181 actgcggcca cacgcttttg atgtagttca ccgtgaagcc ggcttctagc tgaccgatat
    48241 gcagcgtggc gggattgtcc tgccagtagc ggagcatgac atcaacgacg acccgcaggc
    48301 gattggccgg ttcgggatcc tccgcgatgg cttgctgcag cacttcttgc agcttcatga
    48361 cgaaatgatc ccccaacgcc gccaggatct cttccttgtt cttgaagtac cggtacagcg
    48421 tcccggcgga tacccctgac tcagcggcga cgtcgctcat ggtgagcttt ttcgggccct
    48481 gacgggcgat ggcgtgcagc gcgccgttga agatgcggcc ttcggtggtt gccctggtcg
    48541 ccaatagacc ggcctttcga ctgcgagctt cgcgttggtt aagaagactt cgggttgctg
    48601 ccgataataa cgcaaccgaa tgtgatgcac acattgaata actattgttc tatcgatatc
    48661 attacatcca ctagcgtgcc cgacgatcac aacactgagc ggggagcacc ttgggccgcg
    48721 cagtggcacg gggacttgta gagcgactta ggacgatcac cggagctatg ggcgctgaag
    48781 gagcctgatc cgtctacacg aaagcccgac ctggcccgca acgcccacaa ggagcaaaca
    48841 tggcagccga tgctgatgcc gccaacagga aactgaccga ctcgcccaca acgacctcgc
    48901 ccacgacacc aacagcggtg catcgccgta acacgccgct acagaagaaa ccagcatggc
    48961 cgcaagccaa tcggcgacgc aggctagccg gattgatgca gaaaccgaca gccaaccggc
    49021 cgaactcgac tccgacaccg atatcgcggc tgaggcagac gaggtcaccg acgacgagtc
    49081 agcacctgct gcaccggcca gtcgacccgt ctccggtgtc agggtggcgc gctagttttc
    49141 gggctggtcg cggtggtggc tctggcaggg ctgatccgct ggctgggcta tcggactcat
    49201 gagtcacatc acgccaaagc acagcgggag cttttcgtcg aagtggggcg ccaggctgca
    49261 atcaacctga ccacgttcga cttccaggag gtggacaccg acgtgcggcg catcctggac
    49321 tcgtcgaccg gcgcgttaac caaaccatca tcatcggcac tgatccgccc agcaacaccg
    49381 cctccagcgt caaggtgact cttgacaaga tcggaacgcg ctggctgatc tcgggcttcg
    49441 aaccggtctg atgcgccgcc catacggcgg ttcagcagag ttgtcccgca ctcaccatta
    49501 atgccttccg gcgggcacag aactgtttgt agttggcgcg ccggcgagaa cttaggaaga
    49561 ccgttaatga atgaaaggaa ctgccaggaa tgcgtcagat cgaaagccaa gttctcatcg
    49621 ttggcggtgg gggtgccgga ctcacaacct cggtgttcct tgccgatctg ggtgtcgagg
    49681 ccatgcttat agagcgccac gacagtacgt cgagtctgcc gaaggcgcat tatctcaacc
    49741 agcggacgat ggaaattttc gcgctgcatg gaatggccga tgctgtgtat gcgaagtcgg
    49801 cgccacgcgc caacaacgcc aagatcaagt ggctgtcctc tgtgggcggg gatgggcctc
    49861 tggaccgaaa tgtgctagcc gaggccgacg ttatgggcgg cggcgataca gcatctatgt
    49921 ataacgcaaa aggattcagg ccaccaacac acatcccgca gttccggctt gagccaataa
    49981 tccgacaaat tgctcaccag cgccgccccg ctggggttct gttccggcac gagctgcggt
    50041 cgttcacaca ggacgataac ggggtcattg ctatagtccg cgatctcctc gtcgaggaag
    50101 atatcgaggt tcgctgccaa tatctgctgg gcgcggacgg gggacgaaca gtcggcccgc
    50161 agctcggagt atcaatgcag ggaccgaccg gaatggcgga cttcgtgagc gtctggtttt
    50221 ctgcggatct ctcgagctat ctcaccgaca gcgactccgt aatgcgtgtg atcttcccgc
    50281 cgggacaggt cgcccaaggc aagtacactt gcattcttgt cgcatatggc cccacccgct
    50341 gggacgggca cagcgaggaa tggggtttgg ggcttatgcg tagccccgac gatcccgacg
    50401 acgtcgacga gaaatccatc gtgccgatca tccgcgagtt cctcaagatc gacgtgccat
    50461 tgcaggtaca caaagtcacc cattggctcc ttgagacgac ggtcgccaac cgcttctcgt
    50521 tcgaccgcgt gcatctgctc ggcgatgctg cacacaagca gaccccaggc gccgggctgg
    50581 gactcaattc ggcaatccaa gacgctcaca acatcagttg gaaacttgca ctcgttctga
    50641 agggatcggc gcccccgagc ctcctcagct cctatgagga tgagcgccgc cccgtcatca
    50701 cgcaaaatgt tggttggtcg atgttctccc tgaccaacca ccttttggtc gtgtcggcca
    50761 tgggcgttaa cctcgctgct ccacccgagg caaatgaggc tgcgatcgcc gagctcatct
    50821 cggacacgcg aaccggtgcg tcaaagcggg cccgcctagc cgaggtattc cgtacgcagc
    50881 gagtcgaata tcaggcgcat gacatggata tgggcttcaa ctatccggtg ggcgcggtcg
    50941 tcgacgatgg ctctcccagc ccgtggcgcg accccatggg tgccgaatac cggccaacga
    51001 ctcgcccagg ctgtcgtctg ccgcacactt ggctcgaata ccagggcacc agacgctcaa
    51061 ctcacgagct gataccgatg ggcggaatgt tggcgctaac gtccgacgag cccctagcgc
    51121 aacaatggcg ggatgcggta gcgaaggttt ccgccgacac cggcgtgcct atccgaaccg
    51181 tgcggatcgg cccgcgggga gatgctttcg atccgaccaa taattggatt gcacttcgag
    51241 aagttggtcc gaaaggaatc gttctcgttc ggcccgacgg ccacgtcggg tttcgggcca
    51301 tcaacgtgcc gcccaatccg gccgacacga tcgctgcagc cgttcaccac atcctctcag
//
LOCUS       DASYLX010001117         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3157104,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001117 DASYLX010000000
VERSION     DASYLX010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157104"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGP27_26330"
     CDS             <1..606
                     /locus_tag="VGP27_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-dimethylarginine dimethylaminohydrolase"
                     /protein_id="HEV7854853.1"
                     /translation="DMVYAANGGLIVDGKAVVARFAYPERAGESVAYAEWMGRHGFHP
                     IETRHVNEGQGDLLVAGSIVLAGYGFRTDRRAHHEIAAAVGMPLVSLELVDPRFYHLD
                     TALAVLDETTIAYYPPAFTEESRARLLEFFPDAIEVATPDAYVLGLNAVSDGLNVVHP
                     AAATGFAGQLCDAGFVPIGVDLSELLKGGGSIKCCTLEVYS"
     gene            603..>1597
                     /gene="rocD"
                     /locus_tag="VGP27_26335"
     CDS             603..>1597
                     /gene="rocD"
                     /locus_tag="VGP27_26335"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEV7854854.1"
                     /translation="MTILDSVTPTTGAIAVDDRYVAHNYSPLPVVAAGAEGAWITDIE
                     GRRYLDCLAAYSAVNFGHRNPEIIATAHAQLDTVTLVSRAFHSERLGPFCRSLAQLCG
                     KDMVLPMNSGAEAVESGIKVARKWGTDVKGVPAGQGNIIVAHNNFHGRTTTIISFSDD
                     DSARRGFGPYTPGFRSVPFGDAEALAAAIDDDTVAVLIEPIQGEAGIVVPPDDYLGRV
                     RTLCSERDVLMIADEIQSGLARTGRTFACDHWGIEPDIYLLGKALGGGVVPLSAVVAN
                     EEVLGVLHPGEHGSTFGGNPLAAAIGTTVVDILRRGEFQVRSTELGERLHTRLRAL"
BASE COUNT          260 a          548 c          517 g          272 t
ORIGIN      
        1 gacatggtgt acgccgccaa cggcggcctc atcgtcgacg gcaaagccgt cgtcgcacga
       61 ttcgcctacc cggagcgggc tggtgaatcc gtcgcctacg ccgaatggat gggccgccac
      121 ggcttccatc ccatcgaaac gcgacacgtc aacgagggcc agggcgacct gctcgtcgcc
      181 ggttcaatcg tgctggcggg ctacgggttt cgcaccgacc gccgcgcaca ccacgagatc
      241 gcggctgcgg tcgggatgcc gctggtcagc ttggaactgg tcgatccacg cttctaccat
      301 ctcgacaccg cgctggcggt actcgacgag accacgatcg cctactaccc gccggcgttc
      361 accgaagagt cgcgcgcgcg gttgctcgag ttcttccccg acgccatcga ggtagcaaca
      421 cccgacgcct acgtgctcgg gctcaacgcc gtctccgacg gtttgaacgt cgtccatccg
      481 gcggccgcga ctggcttcgc cgggcaactc tgcgatgccg gcttcgtgcc gatcggtgtc
      541 gacctctctg agctgctgaa gggtggcgga tccatcaaat gctgcactct ggaggtgtat
      601 tcatgaccat tctcgattcg gtgacaccaa ccaccggtgc gatcgccgtc gacgatcgct
      661 acgtcgcgca caactactcg ccgctgccgg tcgtcgcggc cggtgccgag ggcgcctgga
      721 tcaccgacat cgagggcagg cgctatctgg actgcctggc tgcctactcg gcggtgaact
      781 tcgggcaccg caatccggag atcatcgcga ccgcccacgc gcagctcgac accgtcacgc
      841 tggtcagccg ggcgttccac tccgagcggc tcgggccgtt ctgccggtcg ctggcccagc
      901 tgtgcggcaa ggacatggtg ttgccgatga acagcggcgc cgaggcagtc gaaagcggta
      961 tcaaggtcgc acgcaagtgg ggcaccgacg tcaagggcgt gccagcaggc cagggtaata
     1021 tcatcgtggc gcacaacaac ttccacggcc gtacaacgac gatcatcagc ttctccgacg
     1081 acgactcggc gcgccgcggc ttcggcccgt acacccccgg cttccgctcg gttcccttcg
     1141 gcgacgccga ggcgctggcg gccgcgatcg acgacgacac cgtcgctgtg ctgatcgagc
     1201 cgatccaggg tgaggcaggc atcgtcgttc cgccggacga ctatctggga cgcgtgcgca
     1261 cgctgtgctc cgagcgggac gtgctgatga tcgctgacga gatccagtcg ggccttgctc
     1321 gcaccggacg cacgttcgcg tgcgaccact ggggcattga gcccgacatc tatctgctgg
     1381 gcaaagccct tggcggcggc gtggtgccgc tgtcggccgt ggtcgcgaac gaggaggtcc
     1441 tcggggtgct tcaccccggc gaacatggat cgacgttcgg cggcaacccg ctggccgcgg
     1501 cgatcggaac caccgtcgtg gacattctgc ggcggggcga attccaggtt cgctcaaccg
     1561 aactcggcga gcgactgcac acgcgtctgc gtgccct
//
LOCUS       DASYLX010001118         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1729473,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001118 DASYLX010000000
VERSION     DASYLX010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729473"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1098
                     /locus_tag="VGP27_26340"
     CDS             175..1098
                     /locus_tag="VGP27_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7854855.1"
                     /translation="MRGRPSRLVAVAAAVVALTGCEARVYGTPPPVDPDAPLLTVVAP
                     QGALAPLPEAAPDEPASSFDGLDVRTKQATADAAAEGADITTVVLDRSTGRRVTNGNG
                     RGIAIASVVKLFIADDLLLRAPPLSPDDLKSFQSMLRSSDDSAAEVFWNRGGGSAIVS
                     RVAARYGLEGTRPPNDGMWFNTISTAADLVRYYDMMLTGSGGLPMEKANMIVDNLAQS
                     SPTGIDGMMPGGVYPQRFGIPEGLYAGRVAVKQGWMCCIGSDWMHLSTGIVGAEHRYV
                     MAIASNQTANADTARAAITQAVRTMFPGGRI"
     gene            1198..2127
                     /locus_tag="VGP27_26345"
     CDS             1198..2127
                     /locus_tag="VGP27_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7854856.1"
                     /translation="MHRRPSSLTTATAVFAIAALLAAGCEARGYGAPPRDSDTPQVTV
                     IAPQSYTAPPPDAPPDQPAATFAGLDARLREATDEAADSGADIKAVVLDRKTGQIVSN
                     GDNGAFPIASVVKLFIADDLLLQESKGQTTLSPADRKSLDTMLRSSDDSAAENFWNRS
                     GGNAVITRVVARYGLTGTTAPYNGHWDVTESTASDLVRYYTKLLDGTGGLPPEQANVI
                     MGNLAQSTPTGADGYPQRFGIPEGLYAEPVAVKQGWFCCWDGDDQLHVTTGVIGPDRR
                     YVMAIGSLEPDDDAAARATMTQVVKTMFPGGKI"
     gene            complement(2124..2801)
                     /gene="trmD"
                     /locus_tag="VGP27_26350"
     CDS             complement(2124..2801)
                     /gene="trmD"
                     /locus_tag="VGP27_26350"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV7854857.1"
                     /translation="MRIDVVTIFPDYLDPLRQSLPGKAIDAGIVDLRVHDLRRWTHDV
                     HRSVDDSPYGGGPGMVMKAPVWGAALDEICSPKTLLVVPSPAGRLFTQSTAHRWSAEQ
                     HLVFACGRYEGIDQRVMDDAAQRMRVEEVSIGDYVLPGGESAALVMIEAVVRLLPDVL
                     GNPASHQEDSHSEGLLEGPSYTRPPSWRGLDVPDVLRSGDHAKVVAWRREQAVARTKD
                     RRPDLID"
     gene            complement(2804..3325)
                     /gene="rimM"
                     /locus_tag="VGP27_26355"
     CDS             complement(2804..3325)
                     /gene="rimM"
                     /locus_tag="VGP27_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559366.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV7854858.1"
                     /translation="MDLVVGRVVKAHGVSGEVVVEVRTDDPDTRFAPGAQLRAQPRGG
                     GPQRTYVVESAREHSGRMLVRLADVADRTAAEALRGSVFLVDTEDLPPIDDPDEFYDH
                     QLEGLRVVTTDGQLVGTVKEVLHTAAGELLSVTAETGAEVLVPFVGAIVTEVSLQSHT
                     IEIDPPQGLLELD"
     gene            complement(3333..3575)
                     /locus_tag="VGP27_26360"
     CDS             complement(3333..3575)
                     /locus_tag="VGP27_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV7854859.1"
                     /translation="MSSVVVDAVEHLVRGIVDNPDDVRVDMVTSRRGRTVEVHVNPED
                     LGKVIGRGGRTATALRTLVAGIGGRGIRVDVVDTDQ"
     gene            complement(3572..>3887)
                     /locus_tag="VGP27_26365"
     CDS             complement(3572..>3887)
                     /locus_tag="VGP27_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV7854860.1"
                     /translation="GDWQKFKGLPGAEGTLKVKEPKPSKLDLFNAALAEADGGPSAEA
                     TTAKKKKAPAKKAAKGETATAEPDAAAIEAHAAPVRDESEPAAEGPDATPDAAAEQAD
                     ES"
BASE COUNT          613 a         1370 c         1287 g          617 t
ORIGIN      
        1 tccccgcgtc tcgcacagac aactggtcaa ttgtgccaga cggggccgca tgcagtgaaa
       61 tcgcgattcc gggcgggcac gcagcgtccc cgccgaccgg ataggctcaa tcctacgggc
      121 ggccaccggt cagctgcgcc cggtcccggt gaagcggatt tctggaggat tcctatgcgt
      181 gggcggccat cgaggctggt cgcagtggcc gcggcggtag ttgcgctgac cggttgcgaa
      241 gctcgcgtgt acggcacgcc gccgccggtg gatcccgatg cgccgctgct gacggtcgtc
      301 gcgccgcagg gcgcgctggc tccgctgccc gaggccgcgc ccgacgagcc cgcctcgtcc
      361 ttcgacggcc tggacgttcg caccaagcag gccacggccg acgccgcggc cgagggcgcc
      421 gacatcacca ccgtcgtgct ggaccgctcg accgggcggc gggtgaccaa cggcaacggc
      481 agaggcatcg ccatcgcatc ggtggtcaag ctgttcatcg ccgacgacct gctgctgcgg
      541 gctccgccac tgtccccgga cgacctcaaa tcgttccagt cgatgctgcg ctcgtccgac
      601 gacagcgcgg ccgaggtctt ctggaaccgc ggcggcggca gcgcgatagt cagccgcgtc
      661 gccgcccgct acggcctcga aggcacccgg ccaccgaacg atggcatgtg gttcaacacc
      721 attagcacgg ccgccgacct ggtgcgctac tacgacatga tgctcaccgg ctcaggaggc
      781 ctgccgatgg aaaaggccaa catgatcgtc gacaacctgg cgcagtccag ccccacaggg
      841 atcgacggca tgatgcccgg tggcgtctac ccgcagcggt tcggcatccc cgagggcctc
      901 tacgccgggc gggtcgccgt caaacagggc tggatgtgct gcatcggcag tgactggatg
      961 cacctgtcca ctggaatcgt cggggccgag caccgctacg tcatggcgat cgcctcgaac
     1021 cagacggcaa acgcggacac ggcaagggcc gccatcactc aggccgtcag gacgatgttc
     1081 ccgggcgggc ggatctagcc agctacgtat taggctggca tcgccggcgg gcgcagtatg
     1141 gcggcagtgt ggcaggctcc cgctccgcag gctcaggttc tgctttgaag gagagttatg
     1201 catcggcgcc cgtcgagtct gactacggcg accgcagtct ttgcgattgc ggcgctgctc
     1261 gctgccggct gtgaggcacg gggttatggc gcaccgccca gggattcgga tacgccgcag
     1321 gtgacggtca tcgcgccgca gagctacacc gccccaccgc cggacgcccc gccggaccag
     1381 cccgccgcga cgttcgccgg tctcgacgcg cggcttcgcg aggccaccga cgaggccgcg
     1441 gattccgggg ccgatatcaa ggccgtcgtg ctggaccgca aaaccggcca gatcgtgtcg
     1501 aacggcgaca acggcgcatt cccgatcgcc tcggtggtga agctgttcat cgccgacgac
     1561 ctgctgctgc aggagtcgaa gggccagacg acgctctctc ctgccgaccg caaatcgctc
     1621 gataccatgc tgcggtcgtc cgacgacagc gcggcggaga atttctggaa ccgcagcggc
     1681 ggcaacgcgg tcatcacccg cgtcgtggcg cggtacgggt tgacgggcac gacggcgccc
     1741 tacaacgggc actgggacgt caccgagagc accgcaagcg atctggtccg ctactacact
     1801 aagctgctcg acggtaccgg cgggctaccg cccgagcagg ccaacgtcat catgggcaac
     1861 ctcgcgcagt cgacgccgac cggtgccgac ggctatccgc aacgcttcgg cattcccgag
     1921 gggctctatg ccgagcccgt cgcggtcaag cagggctggt tctgctgttg ggacggcgac
     1981 gaccagctgc atgtgaccac cggcgtgatc ggaccagacc gccgctacgt gatggcgatc
     2041 ggctcgctgg aacccgatga cgacgccgcc gcccgcgcga cgatgacgca ggtcgtcaag
     2101 acgatgttcc ccggcggaaa gatctagtcg atcaggtcgg ggcggcggtc tttggtgcga
     2161 gccaccgcct gttcccttcg ccacgccacc accttcgcgt ggtcgcctga gcgcagcacg
     2221 tccggcacgt ccagcccgcg ccagctcggc ggccgcgtgt agctggggcc ttcgagtaat
     2281 ccctcggagt gcgaatcttc ctgatgcgac gcgggattgc ccagcacgtc gggaagcaga
     2341 cgcacgaccg cctcgatcat caccaacgcc gccgactcgc cgcccggcaa cacgtagtca
     2401 ccgatcgaca cctcttcgac gcgcatccgc tgcgccgcgt cgtccatcac gcgctggtcg
     2461 atgccctcgt agcgcccgca ggcgaacacc aggtgctgtt ctgcgctcca gcggtgcgcc
     2521 gtcgactgcg tgaacaaccg gcccgccggc gacggaacga cgagaagtgt tttcggcgag
     2581 cagatttcgt cgagcgccgc tccccacacg ggggccttca tcaccatccc ggggccgccg
     2641 ccgtacggcg agtcgtcgac ggagcgatgc acgtcgtgtg tccaccggcg cagatcgtgc
     2701 acccggaggt ccacgatgcc cgcgtcgatc gccttgccgg gcaacgactg ccgcaacggg
     2761 tccaggtaat ccgggaagat cgtcaccacg tcgatacgca tggtcagtcc agctccagaa
     2821 gaccctgagg cgggtcgatc tcgatggtgt ggctctgcag cgacacctcg gtgacgatgg
     2881 cgccgacgaa gggcaccagc acttccgcac cggtctccgc ggtcaccgac aacagctcgc
     2941 ccgccgcggt gtgcagcact tctttgacgg tgccgaccag ctgcccgtcg gtcgtcacga
     3001 cccgcagtcc ctcgagctga tggtcgtaga actcgtcggg atcgtcgatc ggcggcaggt
     3061 cttcggtgtc gacgagaaat acactgccgc gcaacgcttc tgccgcagtc cggtcggcga
     3121 catccgcaag ccgtaccagc atgcggccac tgtgctcgcg cgcggactcg acgacatacg
     3181 tgcgctgcgg gccgccgcca cggggttgtg cccgcaattg tgcaccgggc gcgaaacgcg
     3241 tgtccgggtc gtcggtgcga acctcgacga cgacttcgcc ggatacaccg tgcgccttga
     3301 cgacccgccc gactaccagg tccatacgac cgctactggt cggtgtccac cacgtcgacg
     3361 cggatgcccc gaccgccgat gcctgcgaca agagtccgca gcgcggtcgc ggtacggccg
     3421 cccctgccga tgaccttgcc gaggtcctcg gggttaacgt gcacctcgac ggtgcggccg
     3481 cggcgcgacg tcaccatgtc gacgcgcacg tcgtccgggt tgtccacgat gccgcggacc
     3541 aggtgctcga ccgcgtcgac gacgaccgag ctcacgattc gtcggcctgc tcggcggcag
     3601 cgtcgggagt ggcgtcaggg ccttctgcag ccggctccga ctcgtccctg acgggcgcgg
     3661 catgggcctc gattgcggcg gcatccggtt cggcggtcgc cgtttcgccc ttggcggcct
     3721 ttttggcagg cgccttcttc ttcttggccg tggtggcctc ggcgctgggg ccgccgtcgg
     3781 cctcggcgag cgctgcgttg aacagatcga gcttgctggg cttgggctcc ttgaccttca
     3841 gcgtgccctc cgcgccgggc agacccttga acttctgcca gtcgccg
//
LOCUS       DASYLX010001119         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_83976, whole
            genome shotgun sequence.
ACCESSION   DASYLX010001119 DASYLX010000000
VERSION     DASYLX010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83976"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1260
                     /locus_tag="VGP27_26370"
     CDS             181..1260
                     /locus_tag="VGP27_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV7854861.1"
                     /translation="MRTGISLHVTASDRERLLAVVGDRNTAQKHVWRARIVLLTADGH
                     GTTEIMRQAGVAKTAVWRWQERFMQTGVDGLLRDKTRPPRIPPLGAAIVERVVRLTLS
                     DPPGETTHWTAPAMAKAAGISVSSVQRIWRSHGLQPHRMRQFKLSNDPHFAEKVRDIV
                     GLYLDPPAHTVVLSVDEKSQIQALDRTQPGLPIKRGRCGTMTHDYIRHGTTTLFAALN
                     VMDGRVIGRCMQRHRHQEFIRFLNTVEAEVPAAKLVHAIVDNYAVHKHPKVIQWLGRH
                     PRWTFHFTPTSASWINAVEGFFAKLTKRRLKRGVFHSLVSLHEAINRFVREANAKPKP
                     FSWTKDPDTIIAAVRRGHQALDSIH"
BASE COUNT          303 a          572 c          519 g          245 t
ORIGIN      
        1 ccgggcgggg gagcaggcca tccgtaaata catggaatgg caggcgaacg acgaaaaggt
       61 ggcgcacgcc gagcgggtag caactgaagt cgttatgggg aggtcgctag tggacaaacc
      121 ctgaccgaag agtccgaaaa agaagttctg ccggcgcggg gcggtatgcg aatcgagagc
      181 atgcgcaccg ggatcagcct gcacgtcact gccagcgatc gcgagcggct gctcgccgtc
      241 gtaggcgacc gcaacaccgc gcagaagcac gtctggcgcg cccggatcgt gctgcttacc
      301 gccgacggcc acggcaccac cgagatcatg cgccaggcgg gcgtagccaa gaccgcggtc
      361 tggcgctggc aggagcggtt catgcagacc ggcgtggatg gtctgctgcg cgacaagaca
      421 cgcccaccgc gcatcccgcc gctcggtgcc gctatcgtcg agcgggtggt gcggctgacg
      481 ctgtccgatc cgcccggcga aaccacgcat tggacggcgc cggcaatggc gaaggccgca
      541 ggcatcagcg tcagttcggt gcagcgcatc tggcgcagcc acggcctgca gccgcaccgg
      601 atgcgccagt tcaagttgtc gaatgatccg cactttgccg agaaggtccg cgacatcgtc
      661 ggcctctacc tcgacccgcc cgcccacacg gtggtgctgt cggtcgacga gaagagccag
      721 atccaggcgc tcgaccgcac ccagcccggc ctgccgatca agcgcggtcg atgcggcacg
      781 atgacgcacg actacatcag gcacggcaca acaaccctgt tcgccgccct caacgtgatg
      841 gacggccgcg tcatcggccg ttgcatgcag cgtcaccgtc accaggaatt catccgcttc
      901 ctaaacaccg tcgaggccga ggtcccggcc gccaagctgg tgcacgccat cgtcgacaac
      961 tacgctgtcc acaagcaccc caaggtcatc cagtggctcg gtcgccatcc gcgctggacc
     1021 ttccacttca cgccgacctc ggcatcgtgg atcaacgccg tcgaggggtt cttcgccaag
     1081 ctgacgaagc ggcgcctgaa gcgcggcgtt ttccactccc tcgtctcgct ccacgaagcc
     1141 atcaaccgct tcgtcaggga ggccaatgcc aaaccaaagc ccttcagctg gaccaaggac
     1201 ccggacacca tcatcgccgc cgtccggcgc gggcaccaag cgttggattc catccactag
     1261 ggcgcgcgca agttgcgcga tcgccatgcc accgagggcg cggccggcct gcgcgaccgc
     1321 tcctcgcggc cgcatcgcag cccgacccag ctttcggccg atcggcacgc agccgtcgag
     1381 gccctgcgtc ggcaaaggct cagcggtccg tccatcgccc gccagctcgg cctgccgggc
     1441 tccaccgtcg gcactgccct gcgccggctc ggcctaaggt ctttggggat ttaggattcc
     1501 cttcgcgcgc gagatgtgat tcacgctgtg gatggatcgc tcagtgctga cggacgccca
     1561 atgggcgaag atggaacctc attgccttgg caagccgacc gatccgggac ggagcgggag
     1621 cgacaaccgc ctgttcgtg
//
LOCUS       DASYLX010001120        57626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_744633,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001120 DASYLX010000000
VERSION     DASYLX010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 57626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57626
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744633"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VGP27_26375"
     CDS             <1..441
                     /locus_tag="VGP27_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854862.1"
                     /translation="HLMHNLPSERLSIAISAIAGARETWRQTLQYAKDRKAFGQPIGS
                     FQHNRFLLAEMDTELEIGEQYIDRCLQAVVDGELTAVEASKAKWWCTETAKKVIDGCV
                     QLHGGYGYMTEYRVTRDYMDNRIMTIFGGTTEIMKDIIGRDLGL"
     gene            524..1279
                     /locus_tag="VGP27_26380"
     CDS             524..1279
                     /locus_tag="VGP27_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7854863.1"
                     /translation="MNGISTSRDGGVLRVLLDRPDKLNAVNTPMLIELVARLADAGAD
                     ESVRAVLLTGAGRAFCSGGDLSGADTRGAAQAANEVIRVITALPKPVVAGVHGPAVGF
                     GCPLALACDLVVVARSAFFQLAFSQVGLMPDGGTSALLPAAIGRARAARMALTAEKIS
                     AATAFEWGMVSHVVADAAFETEVTEVTRDVAVGPTQSYRWIKRALAAATLSELESVQA
                     IEADGQQKLAKTSDFRRGTVAFRERRRPEFKGD"
     gene            1293..2522
                     /locus_tag="VGP27_26385"
     CDS             1293..2522
                     /locus_tag="VGP27_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854864.1"
                     /translation="MQKPMTGVRVLEVAQFTFVPSAGAVLADWGADVIKIENPVTGDA
                     QRGLVAVDGRPAMHERTSFAPVVEAPNRGKRSIGLSLALNQSRRAFCELVKRSDVFLT
                     SHLPQIRAKLRIDVADVRRINPDIIYVAGSGYGSAGPDRDTGAYDATAFWARGGSSDG
                     VTPADARAPANMPAGAFGDNIGGITIAGAVAAALYGRRTTGEPSVIDVSLLAIGAWTT
                     QFSVNRAMQYGCSLPKIDRRTQAPGNPLTGAYQCADGRFVQLSMLQPARYWPEFCHLF
                     GFDEAAADDRFTSLESLAANADEATALVADAIGSRNFDECKALLNQSGGQWAPVQDAW
                     DVANDQSLIANGRIANVVDAQGNAQKLVANPVKFDDASPNLTRAPQFAEHTDDVLREL
                     GIGEDELLDLKFDGAIT"
     gene            2533..3963
                     /locus_tag="VGP27_26390"
     CDS             2533..3963
                     /locus_tag="VGP27_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEV7854865.1"
                     /translation="MTWLEHIDAVHRGNDWPAVFDATGPVSGRALIGKAVYAAELLTT
                     LESRPGRAVPALLTVNADALALLLGGAAANRPLAPLGPRLTQAELAAVLFATDARVLL
                     TEPAFAETARAAAQLANVRVVSLESLPSSVGRLDGNCEVAFHLHTAGTTGAPKPVAFT
                     PSVLIARGEVLTELVGFGAGDRYATGSPIHHIGGLGNVLTALGAGAAILPTTYFSAAW
                     WRSLREFGATHCLLVPSMIEMLLRDGLLDAVALRTLIYGASPITEYTLSRVLHTLPGV
                     RVVSLFGQTEGSPITSLGPDDHRRATEGAPHLLGTVGRAVPGLRLTIDSPDETGTGEV
                     LAAASHLSVCGADGWLHTGDLGVIDADGYLRLVGRQHDMVVRGGENVYPVEVENVLCS
                     HPRVAAAAVIGVADSRLGETLAAYIVPLDRHDPPDENDLRSFTRARLSGFKVPATWSL
                     LDQLPRNSAGKLNRLALQQMHDRVGG"
     gene            complement(3960..4706)
                     /locus_tag="VGP27_26395"
     CDS             complement(3960..4706)
                     /locus_tag="VGP27_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854866.1"
                     /translation="MALPDLVLVHGGEHAGDCWEPTLVELRRQAPELRTLAVDLPGHG
                     AKPGNLATVTIGEWVDSVVADIEDAGLGDIVIVGHSMAGVTVPGVVTKLGSARVREMI
                     LATAFVPPQGGAIVDTLGGPLAWFARRAARIGNPMKVPELAARYAFCNGMTREQRRFT
                     LSRLHPESARIPAEGVDRSGLPDEVPRTWILTTRDRALSVSSQRDSIAALGGVQTVIP
                     VDACHEVMISHPELLARILIERCRRWEHPG"
     gene            complement(4725..5636)
                     /locus_tag="VGP27_26400"
     CDS             complement(4725..5636)
                     /locus_tag="VGP27_26400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV7854867.1"
                     /translation="MHYNLMFPMRAVKHWNDWCGGASIGDVAKLVEEAGFDGFSMSEH
                     PYPDKGWLANGGHHAFDPFVSLSFAAAATTRIQVMTYILVSGYRNPYLTAKAAASLDL
                     LSGGRFTLGTGAGYLKSEFEALGADFGRRGALLDEAIEAWRATWACRDHDGPDFGVQG
                     HLALPPPRTEGGPPIWIGGNSNAAQRRIVEVADGWMPMAASGEMAAITRARPLEDIAT
                     LADWIAGINKRRAEFGRCAADVSFVPFEAELLTSGDSTAFCSEVTPQLDAYAEAGVTW
                     ITIEPASRSLADFRADIDMLASQLVHR"
     gene            complement(5649..6827)
                     /locus_tag="VGP27_26405"
     CDS             complement(5649..6827)
                     /locus_tag="VGP27_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854868.1"
                     /translation="MPSPAGQTGAWELPDELVMLRDTVRRFMDAHVHPIEEKLDHDAI
                     GLPRERLVELQDKARALGLWALQTPEEHGGAGLSVLGQVVVAEEAAKCRMGAFFPALG
                     AFGGNPPNIMFKASPQQFARYARPIIDGTMTKAYTAITEASGGSDPVRAIQLRAIRDG
                     DTYVLNGSKMWISHAPDADWGVVYARTGEGRTGISAFILEKNTPGVIFSRIGVMASFA
                     PYELHFDNVRIPEEQLIGEEGHGLSLAGDFLVHGRIIYAAGPIGIAQSALEMACAWAQ
                     DRDVFGGKLADKQGIQWMLVDSEVELRAARLLMYQAAWNADLNRDVRVDASVAKMFGT
                     EAAYKVLDRCIQIHGALGLSDELPLERWFRDLRVKRLGEGATEFQREVIARSLLKQAR
                     "
     gene            complement(6829..7419)
                     /locus_tag="VGP27_26410"
     CDS             complement(6829..7419)
                     /locus_tag="VGP27_26410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7854869.1"
                     /translation="MTIDHEAANAVLAGLRPFPVAVTTIDDGFANGLISLSAGAAGIV
                     PELPRATVSLTKYNRTHDMLINSGIFVMHMLSADPGEVEKSLEILMTLGGSSGRDGDK
                     MSRLRTKAGVTGAPVLLDAHSYVECRVTGSLDNDENTIFVGDVVAAEVFSSGQRLRIG
                     EAWGKLPHEWIERYEANHEPQLQSARDLRAAATSRV"
     gene            complement(7462..8952)
                     /locus_tag="VGP27_26415"
     CDS             complement(7462..8952)
                     /locus_tag="VGP27_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEV7854870.1"
                     /translation="MTDTATQRLVEFELLIGGEPTAAASGATYDSVDPYTGRPWARVP
                     DGAAADVDRAVAAARSALNGPWGQLTATDRGKLLWRLGEIIAREAENLAGLEVRDGGK
                     LVREMVGQMRSLPDYYFYYAGLADKLQGEVLPTDKPNYLVYTRHEPVGVVGAITPWNS
                     PLLLLTWKLAAGLAAGCTFVVKPSDHTPTSTLAFAKLFAEAGFPPGVINVVTGWGPET
                     GAALASHPDVDKVAFTGSTATGISVGKAAIENMTRFSLELGGKSAQVVFADADLDAAA
                     NGVIAGVFAATGQTCLAGSRLLVHESVADALVERIVTRAATIKLGDPKDPSTEMGPMS
                     NQPQYEKVLSHFASAREQGATVAYGGEPAGELGGFFVKPTVLTDVDPSMRAVAEEIFG
                     PVLAVMTFSDEDEAIAAANATEFGLAGSVWTKDVHRAHRVAAKLNTGTVWVNAYRVVA
                     PHVPFGGMGHSGIGRENGINAVKDFTETKAVWVELSGATRDPFTLG"
     gene            complement(8962..9747)
                     /locus_tag="VGP27_26420"
     CDS             complement(8962..9747)
                     /locus_tag="VGP27_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7854871.1"
                     /translation="MTDLENLRTIDFAVSEHVATVALNRPEKLNSFTEEMADELSAVW
                     AKVRDDDDIRVAVLRANGDRAFCTGIDVSEGTWWTHKPIFNQEDPGELLGPKAHRVWK
                     PVIAALHGIVAGGAMYFVNECDFAICSETATFFDPHANAGIVSALEPMGMLALGVPYG
                     EVMRWALLGSEERISAQTALRIGIVTEVVPDDQLRSRATELACEIAARRPLSVQGTVR
                     AMWEARDLAPSTAARHGKFYTDLGNPGAGRTDSRANKKKPRTR"
     gene            complement(9740..11275)
                     /locus_tag="VGP27_26425"
     CDS             complement(9740..11275)
                     /locus_tag="VGP27_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854872.1"
                     /translation="MLISDISTNNARRYPGKRALVEAGRTRTWSQVDERSNRLANHLA
                     GTALVPGDRVMVIARNCIEWPEISFGLAKAGLIAVPVNIRLAPEEVAHVRDDSGARAA
                     IIHADQYQTFAAQVDGLAVKLTIGPEYESVLSTAEPVAPSVAATPDDVAVVLYTSGTT
                     GRAKGVMHTHRGLLYQAADTNLVTEADRSDVMLATTPFFTAGGMVRTVSWLYLGQTMV
                     IHQRFDPHAVIDEIERSSITFTTFIPTMLHRVLAILENGPHRDMSSLRRISYGSAPVP
                     PGLARKAMDLLGCDLQQRYGLTECGGQATILTPQDHRDIVAGKTSIATSCGKETPMCR
                     ITVVDGNGNDVPGGEVGEIVIVSPANAVGYWNRPTQTAETFRSDGLLTGDLGYLDDEN
                     YLHITGRKTDMIISGGFNVYPAEIERVIAQNTGVDLVAVVGVPDPDWGETPVAVVIPK
                     SHVNDRAALTAELTALCRAELAGYKQPRRFEYRDEFPLGPAGKILKREIANQVTHVGS
                     ARD"
     gene            complement(11278..11793)
                     /locus_tag="VGP27_26430"
     CDS             complement(11278..11793)
                     /locus_tag="VGP27_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEV7854873.1"
                     /translation="MTVKTQPESTPVLRPLPDADVLSFLYLESRLADEGRYSEWESLW
                     AGDDENVLYRVPMHPDDDARTTLAYINDNRRRIKSRVAQLNTGNRHSQTPPSVMRRLL
                     SNSEVVEQIDDTVTVESNFALFEYRVRQRFWAGRVVHTLRRSPNGLRLVRKIVHLIDA
                     AGPIDTLAFLI"
     gene            complement(11798..13090)
                     /locus_tag="VGP27_26435"
     CDS             complement(11798..13090)
                     /locus_tag="VGP27_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7854874.1"
                     /translation="MTTLETTTSAEDFDAATVVRSDRVHGSVYTSPEIFRREMDTIFK
                     TGWVYVAHESEVSEPGDYLTRMIGSDPVVVVRGKDGVVRVLLNRCTHRANKLCNADKG
                     NANSFRCPYHGWTFNNTGALQGVPMREGYGEAFAEVRADLGLAQAPRIDSYGGFVFAS
                     LAPDGISLREHLGGATHAIDRLLNLSPDRSIDLRANWMKHLHHANWKMVVENNVDGYH
                     ALFTHASVYDAIKPAKVSHVPTKVDVLVRDIGGGHSEIDYSDEYRKLDEEFVWYGRIP
                     RAKLAGYVDALERAYGPDQAHDALVVGPPHTLIWPNLFLAEMNVMFVEPQAPDRTIAY
                     TTAVLIPGHDDLNERTLRRSEGAMGPAGFLIADDGEIGMRNQAGLAAELPEWLMLARG
                     MDDDITDETGIINRDKSAETPQRGFYSQWAAVVGGGKT"
     gene            complement(13087..13533)
                     /locus_tag="VGP27_26440"
     CDS             complement(13087..13533)
                     /locus_tag="VGP27_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7854875.1"
                     /translation="MTALTTSAREDWLLERALAPAINGDALRPLYDAAARGDLALPFC
                     AGCELALELEQQTCDACGRTEPVWRQVHPRGIVHAATMMHRREAGLVRATDPYPIVDV
                     EMDSGHRIIMTTLTPTDTAPRIGTTVHIAFRRLGDVAIPAVNLEAE"
     gene            complement(13530..14687)
                     /locus_tag="VGP27_26445"
     CDS             complement(13530..14687)
                     /locus_tag="VGP27_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7854876.1"
                     /translation="MSDRDSVAIVGMGMTPMSLVPGGTPLQLACEATASALADAGIEL
                     ADVDGLLVGSSQGVRPDRLGVGFAAQGGFGNLRLLEHVEIKGATTIAMVERARKAIAD
                     AEAATVVCVFADAPLVADKGSGSTYAHSGGNNGVRGMERASGLLGSVPTYALLAQRWL
                     HVTGTSADALAAVATTARRWATGNPDAVSREPLDSDGYAASPMIAEPLRLLDCARPVN
                     GAVAVVLSGRDSIGAARLRILGTGRDHPVRRRRAGAESWFGGGGRAVDDALDEAGITR
                     AELDIAELYDPFSIVTLALLDEYRLTGDTPAGAFMQDGRAGPGGVLPVNTGGGQLSGF
                     YLQGMTPLAEAVIQLRGSGGDRQVPDATVALVGGIGGRMDHHAALILERAA"
     gene            complement(14684..14932)
                     /locus_tag="VGP27_26450"
     CDS             complement(14684..14932)
                     /locus_tag="VGP27_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854877.1"
                     /translation="MDASKGNPKRRELAILINEFAAVRLWLDRNGNGPRLLVEDLETG
                     DEAFLDPLELSSFTVATAEDREDWLRVGNYRGERKPRE"
     gene            15127..16035
                     /locus_tag="VGP27_26455"
     CDS             15127..16035
                     /locus_tag="VGP27_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854878.1"
                     /translation="MTDTAVPGAVIDTDDYRSIWMYLKELEFRQGFTEVRVNGAAVRT
                     RYAEAGSPDKPHIVLLHGTGGHWETFAPNLAALSEHFHCVAIDMIGNGFSDKPDYDYE
                     IAVYVEHVLGVMDHFGMARAHLIGMSLGAWVASTIAVSHPDRVEKVILMSPAGKEATA
                     SNMARIRAERTKAVNEPTWESLHAVFAHLIAEEANRLPDLIGLRQAVYQRDDTRKTID
                     RLLILQDAAARDRNLIPEEKWRGITAPVMIVASGKDHGVYQETAASIAGLIPDSEVFE
                     MPDVRHWPHFEDPESFNPAAVSFLRR"
     gene            16032..16856
                     /gene="hpaH"
                     /locus_tag="VGP27_26460"
     CDS             16032..16856
                     /gene="hpaH"
                     /locus_tag="VGP27_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HEV7854879.1"
                     /translation="MTRPGPGEIADIARRLYTAEQERKPIRQLSLDYPEMTIDDAYAV
                     QRELVDLKVADGRVVKGRKIGLTSKVMQRAVSIDEPDYGALFDDMFVDDGGRVPPGRF
                     IRPRVEVELAFVLGETLRGPDVTLSEVLRATESVRPAIEILDARVQMSDPETGHLRTI
                     VDTIADNAADAGLVLGGREFRPLDVDLPWVAAVLRRNGTIEESGVAAAVLDHPGNGVA
                     WLANRLAPHGVSLCAGEVILSGSFTKPVFAEPGDIFVADYGPQGTVSVAFDREPQR"
     gene            16853..17626
                     /locus_tag="VGP27_26465"
     CDS             16853..17626
                     /locus_tag="VGP27_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEV7854880.1"
                     /translation="MTNRWAERIGDGRPRFGLWVASGSSYVTEICAGSGIDWVLIDQE
                     HAPNDLRTTLDQLQVLAGYPDVDVVVRPPAADPVYIKRLLDIGAQNIIVPMIDGPEEA
                     AAAVAATRYPPAGIRGVGSALARASRWNRISDYLAGADQGVSLTVQVESTAGLAALGD
                     IADVDGLDAVFIGPADLAASMGRLGQPEHPEVVSTIETALNDIVEHGKSAGVNAFNPT
                     IAQRYLAAGASFVLVGADVALLARGADQLAAQYRDGREP"
     gene            17674..17784
                     /locus_tag="VGP27_26470"
                     /pseudo
     CDS             17674..17784
                     /locus_tag="VGP27_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            18184..19356
                     /locus_tag="VGP27_26475"
     CDS             18184..19356
                     /locus_tag="VGP27_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854881.1"
                     /translation="MDLEFTPDQEQFRDRVRTWLDENKPTEPRPRDDEGIREYDLAWQ
                     RTQWEGGWAGIAWPTAYGGKGLTLLQQLIWYEEYAARGFPGIDACFVGLSHAGPTLIT
                     RATEDQKSFHLPKILRGDVIWCQGFSEPEAGSDLAALRTKAVIDGDELVVSGQKLWTS
                     FATVADYQELLVRTDNSGSKHTGITWVICDMSTPGIEVRPIETIEGGSEFCEVFYDNV
                     RIPMSNVVGEVGEGWSVAMATLSFERGTAFTANQVRLAKIIEDLIDYARDHVGPDGRR
                     PAIADDEIARRLARARASVASLRALTYTNICEAMKTETPGPRGSIVKLMYAELAKEIG
                     KLAMDVVGPAAIRRSSRWDADGWAGYYYYSFSQAIGGGTSEIQRNIIGERVLGMPR"
     gene            19369..20391
                     /locus_tag="VGP27_26480"
     CDS             19369..20391
                     /locus_tag="VGP27_26480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7854882.1"
                     /translation="MDLLPGPEQLELVSAAGEFLSDRMPIERIRADRDAQWSVSQRIW
                     REGAELGLLTLGLPEEFGGSGRPLGDEALLHIELGRNLAPGPFLATTLAARIAAKCGD
                     SALADQIGSGKTLVSLAVLRGDGDVRPIKGTFDLFDHVDARYALVVAREGAALADIAS
                     FGEPVVIESADPGTRMATATVQSAEPVHWLSADDEWIWGRAVALSAAFLTGLASAATE
                     QATEYAKTRVQFGKPIGVHQAIKHTCVNMEIAAQAAHAQTLFAAIALESGRSDALLQL
                     LSAVTVAGSAAVDNAAAGIQVFGGMGYTFENDMHLYLKRAHVFRHLFSEPTDVLAELL
                     AQDRAQ"
     gene            20388..21539
                     /locus_tag="VGP27_26485"
     CDS             20388..21539
                     /locus_tag="VGP27_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005573268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV7854883.1"
                     /translation="MNAKVAIAGVALSDVGRVDDKGPYELMAQASRLALADAGLTPAD
                     VDGLASTGQGTLPPVDVGEYLGLRPRWVDSTAVGGASWEVMASHAADAITAGHADVVL
                     LTYGSTARADLKKGLRGANINWGARGPLQWEAPYGHTLISKYAMAARRHMHQYGTTIE
                     QLAEVAVSARFNAADNPDAYYREPISIDDVLDGPMIADPFTKLHCCIRSDGGAAVVLV
                     NADRARDLRSRPVWVLGAAETTSHMLTSQWDDMTVGPAAVSGPLAFARAGVRPADVDV
                     AELYDAFTYMLLLTLEDLGFCPKGEGGRFVEKGSLRLGGELPTNTDGGGLSACHPGQR
                     GLFLLVEAARQLRGECGPRQVPDAKIACVSGTGGWFCSSGTMVLGAEEP"
     gene            21540..23009
                     /locus_tag="VGP27_26490"
     CDS             21540..23009
                     /locus_tag="VGP27_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7854884.1"
                     /translation="MDQPPLCADLGNTRSMLEAAAQVHGDREAYVEPGARISFAEWIE
                     RARAVAAQFLELGVDKGDVVALWLPSGIDYATCHAAAAMIGAITTGLNPRLGPREIES
                     ILSQADPSLVVADERLGAIPAGGRRVLPRASMSTAPVHGDLPAVELNPHDPVSLIFSS
                     GTTGLPKGAVYDAAGLAAGAMSAGVMSAPFDRRLTSTPFAHAGYMFKLWDQLTWGTTL
                     VVPPTPWSAAGMFDVLRTERITVAGAVPTQWAKLLEIEDVGRESLPHLRIGVVATAPA
                     SPELVRRVRQRFGVPLVVRYAMTECPTICGTAPDDPPEIQSATVGRPAAGMEVRVDDG
                     GVVEVRGPCVMRGYWRNPVLTAEVLGEGWLRTGDIGTLDGNGYLSIVGRTGDMYIRGG
                     YNVHPVEVERVLAAHPDVKEAVVVGRSAPVIGEIGVAFVVPIDPRNPPTLQELRAWVS
                     RQLADYKAPDDVVVVDALPLTPMLKPDRAALRAATPRSS"
     gene            23130..23948
                     /locus_tag="VGP27_26495"
     CDS             23130..23948
                     /locus_tag="VGP27_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV7854885.1"
                     /translation="MGNLDGRVALITGAARGQGRAHAVRLAIEGADIIAVDICRDIDT
                     MDYPNATADDLEETVKLVENEGRRIIAHQADVRDADALDQIVGDGVKDLGRLDIVIAN
                     AGIIRLGGGGDRRQIFRDIVDVNLFGVWNTVEAAIPKLIEGGRGGSIVLTSSSAGLKG
                     PNTDRAGAQAYTASKRGLVGLMQVWANHLAQHSIRVNTIHPTGVATGMVMNETMAALV
                     AAEDVAITAMQNALPIPVLMPEDIAGAVAFLVSDEAKFITGTAWPLDAGFAVRS"
     gene            complement(23957..24493)
                     /locus_tag="VGP27_26500"
     CDS             complement(23957..24493)
                     /locus_tag="VGP27_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEV7854886.1"
                     /translation="MITALRDFLDGVAAAAPTTETTVALTEDLRAWTDRLAGAAVPER
                     RQIFARRLDLPGRGQTMSPNFVPITGDHTKVEGRVTFGRYFLGGGGAVHGGAIPLLFD
                     EVLGRLASSGDRAPARTAYLHTDFRSITPVDEELQVSAWFVSEEGRKRILRARLTHGD
                     VLCAEAEGLFIELRPDQP"
     gene            24720..27173
                     /locus_tag="VGP27_26505"
     CDS             24720..27173
                     /locus_tag="VGP27_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7854887.1"
                     /translation="MPEVDLAPHGPLAGLVVVDLSTTLPGAQATQFLADCGADVIMVE
                     PPDGSPLRHLAGWPALLRGKRSITLDLHDDSDLYRLRALLRRADAMVNTLRPAAAERL
                     GLTPELLAERYPRLVVGAITGWGSTGPWRNYKGWEALVMAKTGVMHEKRGLTARPGPA
                     YIAAAYASWGAAHAAVQGILAALLERESSGRGQIVETNLVTGMGSMDPYNWFYEMVLE
                     RYPGAFEPMDAAYDDQGRPQAYLIYALLVAPTKDGRWLQFAQVSPRLIGAWLTELDLM
                     GEIADPKWQGFPMLPTPELRTEWWDLMLERVGARTLAEWQQAMEVNKDLSGELLRSPD
                     DSLNHPQTAHEGRAVTVVDPDLGPVRQPSTLIHADGTPLTTLRPAPRLGEHNDSVSFD
                     EATGAAPPPQDPASDPPLAGVTVLEFGSMFAGPYGATLLADLGARVIKVEPLEGDNIR
                     TLVAFPEAGGGKVLQGKESVAIDFTKPEGLELVYELAKLSDVVLQCFRGKAAERAKID
                     EASLRAVNPDLAFVTTSGYGVDGPFSHRAAYAPSIGAASGLSLIDSQDDGHAPVDLDD
                     THRRAVKLHAGGAVPAVQSDGIAAHGVGSALMVALYAKRRGIQLHNLVTTMLGTVQQA
                     LVVHNTGYAGRPTMQAADDQHFGVTALYRMYRAADGFVFLAAPLPHEWPALAKAMAPY
                     VELHVDERFASAESRAEHDEALIEALTPVFATKSKVEWEDELTAQDVGCVEVAESNSE
                     LVLQTDPYFEAGYAVQAYSPIFEEHRRLAPLCRFSRSRSRAEAGCTIGQHTDAVLREI
                     GLGDARIADLRSREIVGGS"
     gene            complement(27177..27863)
                     /locus_tag="VGP27_26510"
     CDS             complement(27177..27863)
                     /locus_tag="VGP27_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854888.1"
                     /translation="MANVGAELTAVTRRRNSSGESTRVMLMEVAERLFATKGIAAVTL
                     REIQQAAGQSNTSVIRYHFGSRDGLIRALISYRQASLGADRQEMLASMREQGKEADPR
                     AVVWLLARPLANSIAAGEMFAPFLARLVEDPRARSDYWPDHVEDEWTQERLEELVDAA
                     LQDLPERIRRGRTFQLYVSLINVLAAAARSGHGLSEAQLHNYVDTWVGMLTAPVSYET
                     RALMSGSADA"
     gene            27959..29095
                     /locus_tag="VGP27_26515"
     CDS             27959..29095
                     /locus_tag="VGP27_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7854889.1"
                     /translation="MSLIWTNSGDSHFLEPDDLWQSRLPKALADLTPRAEKDPDGEYE
                     TVSVDGQIFRRKLPSSAMTAFIEESMKPQGIRDAEARIADLDKEGVWGEVIFPSLGMW
                     GSTFRTPELLKACMRASNEWALEEICAVSPRYVVTAQVSTLVVEDAVEELHWAAEQGF
                     KAVFLPTTPHPSAPDWHRKEWEPFWAAAEEAGMVIAFHIGTDPVDMTVGGASGGAGQV
                     YRGPGGAIMNYTETTFSGQRAAMKMVAGGALDRHPNLKVLISEGGATWVPFLGDRLLE
                     GYRQHHMAVRPKLSRNPKEILFSQVYASFQHDETAVAAYDHMGYKNVMFGSDYPHMEG
                     TFGHTQDTLKGLFDGVSDETRLRITQGTFYELFPDVPPIPAEKF"
     gene            29100..30299
                     /locus_tag="VGP27_26520"
     CDS             29100..30299
                     /locus_tag="VGP27_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEV7854890.1"
                     /translation="MANTSGLRDAAIVGVGATPYYKRGTSLPKTTTELGCEAILAACE
                     DAGVAVTDIDGFAYYSGASAGYTEKMDTADFMETLGIPEVRFTAALTSGGGGSAGAIG
                     LARAAIVAGDANVVVTLMALQQSNQRLGTVFSAVPPDPMTSFLQPAGLSGPGHLMSVL
                     ARRHMHLYGTRREAFAEIAMSTRVNAMNRPKAMHREPLTLEDYFNARMIAEPLCLYDF
                     CQETDGAVAVITTSAERAKDLRQPPVPVVAAAHGGVRDWGRAFAWMGMPDEYFASSGN
                     KPIATRLYQQAGITADDIDVALLYDHFTPMVLMQLEDYGFCAKGEGGPFVESGAIRFD
                     GGAIPVNTHGGQLSEAYIIGMTHIMEGVEQMRGTAINQVPDAKLALVTGGPASLPVSG
                     LILGKAA"
     gene            30296..30742
                     /locus_tag="VGP27_26525"
     CDS             30296..30742
                     /locus_tag="VGP27_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7854891.1"
                     /translation="MSIVEATLATTIPGEHVCIAVNKDTEPFWAAAKDRRLVAPQCAD
                     CGTFRLPPTPFCPNCQSKQVDWVELSGHAIVYSFAVVHGFPGMPDLLLVPAVLDLPDA
                     PGARLVSNIVDVAPTDVSIGMSLRVDFSPISDGWMLPIFRADTAIA"
     gene            30755..30961
                     /locus_tag="VGP27_26530"
     CDS             30755..30961
                     /locus_tag="VGP27_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854892.1"
                     /translation="MSDDSALHEKPLEFLTGIANTGGSCDGQAILPENGHYRCTCSCD
                     NWDIVVDDPQEGLRQARIHTGSVS"
     gene            30977..31558
                     /locus_tag="VGP27_26535"
     CDS             30977..31558
                     /locus_tag="VGP27_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854893.1"
                     /translation="MTDRAEVEREFRRYFMTGPVLEDWHAWASLFTDEATYFDHFYGT
                     FTGPEEITGFLEGTMGAAPQVYSPLLWNVIDGERVAYKVFNRADNPEPGGSPIEFSSY
                     QFIEYAGGGKWRSEEDVWVLAEMKRFASQYAEAEAANPQSLQQKLRREDWGPWVEWAR
                     PEPEHQANPSWLGKEGFTPFSSIRDIDFGVRSH"
     gene            31624..32538
                     /locus_tag="VGP27_26540"
     CDS             31624..32538
                     /locus_tag="VGP27_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7854894.1"
                     /translation="MTQAAWAFLPDRPADASAVLLCLAGGTYDKHYWHIEIEGHPGYS
                     FGQHLADAGFIVIAVDHLGIGASTDPVASGSLGLHLLAAGDAEVARQVREGLRHGTLL
                     DGVPPTATPLVGVGHSMGACLTTMVQATTHPYDAVVLLGYGVQITNVYEDTADAAELE
                     QRIQQTIEAACQLTGANPGDSHTIAPRDYLAELFYAGEVPQAVVDADTSAQSRVPVRA
                     AAEVTTPGFVEHYTASVDVPVLLAFGAATDVSPNPHAEPANYTSSADVTLHLVPKSGH
                     CHNFSSHRAQLWDRIGVWVRTVTSGSIE"
     gene            32535..33341
                     /locus_tag="VGP27_26545"
     CDS             32535..33341
                     /locus_tag="VGP27_26545"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854895.1"
                     /translation="MTKVIGFIGAGQLGEPMVSRLLAAGHDVLVYARRAEVRDRVASQ
                     GAALADSVADLARRSDILISCLFSDAQFREIGLGPDGVVANARPGAVLVSHTTGTVST
                     LTTLRDSHPSPPLILDAPVSGTVESINAGTLTVLLGGPADAVQMVTPVLAAYADPIVP
                     TGELGSALAIKLVNNLLFSCNAQLLAAAMQLGQALGVGDDALLSALQVCSSASHAAAQ
                     AHRVGGIQQFTERATPFLRKDIAAAAEAAGEAGVDLGLLGVVADSGPLEL"
     gene            complement(33358..34443)
                     /locus_tag="VGP27_26550"
     CDS             complement(33358..34443)
                     /locus_tag="VGP27_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV7854896.1"
                     /translation="MAYTPARGELGGGVREVLRSWLPARLAPAGAESFDISSFSSPDA
                     GYSGKTVFVTAEWTELTGRHRTEDLVLRMQTDSHQLFTTPDAPRQAEVMQRLGAHGVP
                     VPRIVGIERDASVLGSPFYLMRRVYGRTPSDVPSWHKRGWTAELSVSDRELLCDNALR
                     ELVAVHRVTDEADFEFLRTGSPEGPTALQRYLQTLSYWYEWCGDDVKVGAQTLARAWT
                     VLLDSAPRTDAETVVWGDARVGNMCFADDLSVVALFDWETATTGPPDIDLGWWLMFET
                     FLCESLGFTRPPGVPDDDEFVRRYREFGGRLSGDLGYYQLLAAFVLSLINNRLAVLLV
                     RDGLDQATARSYPQASVGLVEHYLQQA"
     gene            34491..35525
                     /locus_tag="VGP27_26555"
     CDS             34491..35525
                     /locus_tag="VGP27_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV7854897.1"
                     /translation="MAGVESSRVALGAYTLPGRVSNPRAAISQAAAAEQLGLRTLWLS
                     ERWGTKDLGVLAGALSQVTTTIRIASGITHFQSRHPAVLASLAMTAQAMSDGRMMIGV
                     GRSVDAMWTAVGLPPSTNASLIDHADIFRRLCRGEKVRYDGPAGTFPSLRLNDVPDQP
                     VPPLVLAAIGPRGLAMAGRHFDGVLLHPFLTPDAVRRSVRVVRDAECAAGRPGGSVGV
                     YATVVVACDLPPGEQSSVVGARAVTYYQLPGLGERLAEVNNWDPDALTRLRSHPILAG
                     VKGSADSVLTRDQLVDVVSSLPDSWIADAAAVGSARECFDVFERYRDAGADELVLHGM
                     TPDRMASLLS"
     gene            complement(35539..36462)
                     /locus_tag="VGP27_26560"
     CDS             complement(35539..36462)
                     /locus_tag="VGP27_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854898.1"
                     /translation="MTGLLDGKIALVTGAGHGIGRGHALELAKHGATVIVNDLGTTLA
                     GEGTGKVADEVVAIIEDRGGKAVSDFSDVGDEEQVDLAVERAYSQLGRLDIVVNNAGI
                     VRDKAIWNMSVDDFDLVMRVHVRGSWLTSRAVARKWRVESKETGSKVYGRIINTTSGA
                     GLHGHFGQTNYSAAKAAIVGLTQTLSLELASIGATANVISPGGRTRMSATMPGAAAPI
                     EPDERAEDEFDPKDPSLGSPVVAWLASPEAGHVSGQVIRALGENLQLLKGWQPVTTVS
                     NNQKRWDADKLGTIMATDIFGTRNTGLRLGS"
     gene            complement(36459..36884)
                     /locus_tag="VGP27_26565"
     CDS             complement(36459..36884)
                     /locus_tag="VGP27_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEV7854899.1"
                     /translation="MARADIPIIDAASAPYWDGARQGRLLIAECGACGTVHHYPRPFC
                     PHCWSDDVHPLRASGTGTLYTYSTVYANDLPPFRERLPYVAAIVELAEGPRVMTTIEG
                     AQPEALRVGMAVTASFRPVDADDPESPYLTVFTPSEEPT"
     gene            complement(36887..37690)
                     /locus_tag="VGP27_26570"
     CDS             complement(36887..37690)
                     /locus_tag="VGP27_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7854900.1"
                     /translation="MSNEQTILSDMAGGVMTITLNRPQAANAVRPEDRDALIALLGAA
                     DGDEKVRVVVLRANGRHFCAGADVGSLADRRATVEKRVMDPMRRIMNGAQKLVASVLD
                     CNKPVIAAVQGAATGVGAHIVYASDLVVATENAYFAESFVKRGLVVDGGGCYLLPRRI
                     GMQKAKEMAFFGERLTASEALTLGLVNRVVPDDELDTAVADFADRLAAAPTTAVAFTK
                     RLLNDSPDTDRAGAFVAEAMAQEVQSYSHDSKEGVQAFIDKRPTEFTGG"
     gene            complement(37687..38946)
                     /locus_tag="VGP27_26575"
     CDS             complement(37687..38946)
                     /locus_tag="VGP27_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854901.1"
                     /translation="MLVHTDEREFAPLIDLGWWQECPAERPDLYRRLREAGRPVFVRT
                     NRQDAPKARGFWAVGAHVDVVDISRRPTEFSSGQGTQIFDQTPEMREYRGSIIDMDDP
                     EHMRLRKIVSRGFTPRMLSELRGLVEETTAEILDEMPASGRCDFVADFATLLPLRIID
                     NMLGVPREHEQFILRATNVVLGASDPEYVPDQTAAGIEAAITETSEELIELLKGIAED
                     RIARPTNDVISKLVTSDEENLTPQELAKFFILLIGAGNETTRNALTHGLHILSGHPEQ
                     RNRLVADYETLASTAVEEILRYSSPVIHMRRTITRDGVALESGTHTFNAGDKVVVWYP
                     AANRDPALFADAEVFNIARTPNNHVAFGGPGPHFCLGAHLARLELNVAFKMLFDRYPD
                     IASVGEPVMLRSNFVNGIKHLTASYTP"
     gene            39081..39872
                     /locus_tag="VGP27_26580"
     CDS             39081..39872
                     /locus_tag="VGP27_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854902.1"
                     /translation="MDLGLRDKAYVIIGGSAGMGLAAGRVLAADGASVVVVGRDQARA
                     TDAAAALADSGAATAFGLAHDASRPGEAVAAVGEAVGLLGRLDGIAITMGTAGMMPID
                     SDDEAWEAAFRDVLLATTRSVQAALPHLADTKGSIVTTAAYSARAPHEPRLPYASLKA
                     AVSTFTRGIAREHGKNGIRANCIAPGAVETDALHAIRGYIAENKGYPYEEALERGLVE
                     DFGFDAALGRPGQPDEVGALIAFLLSQRCGFLTGQTIGIDGGAPY"
     gene            complement(39921..41105)
                     /locus_tag="VGP27_26585"
     CDS             complement(39921..41105)
                     /locus_tag="VGP27_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV7854903.1"
                     /translation="MARYRLLPTTEQQVELAEHCRHARYVWTLAVEQQLHWRPGRHAP
                     GYHEQCAQLTAARAEYDWLAAGSQTMQQQALRDFAQAMRNFFCGTHRRPRWRKAAVHE
                     GFRQVGVKSYQVERLNRRLGRVWVPKVGWVRFWLSRPVPEGAKSYRVTRDRAGRWHIA
                     FAHVPGPITGPSDGSVVGIDRGVVVSAALSTGELLHAPSLTPGESKRLRVLQQRLGRA
                     ERGSHRRARTKLAIAKLKARETDRRKDWVEKTTTDIARRFDTVRIEALDVRAMTRSAR
                     RTVEQPGVRVAQKRSLNRGISRSGWGLLAARLLHKASGRVEQIPAAYTSQRCSACGHV
                     ALENRKSQAVFECEACNAGLCNADVNAARNIAAGRAVTARRDLGASRSANREPQLCSP
                     AA"
     gene            41204..42292
                     /locus_tag="VGP27_26590"
     CDS             41204..42292
                     /locus_tag="VGP27_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7854904.1"
                     /translation="MNLRVGIIGVGWGSHVQVPGFRATDGFEPVALCARTPERLERVA
                     AKLGIADTSTDWESFVTRDDLDVISVATPTVLHRDMTLAALDAGKAVLCEKPLAGDLD
                     AARDMVRAADKSSRPTACCFENRWNPDWLAVADLVGKGFLGKQYLARVSRSASYWHPS
                     HQPQARWMYDRDQGGGYLAGMMVHDLDFLCSLLGRPESVCAEVRTSEPVRELPDGETL
                     TVTADDTAALLMRMESGITAILSVSVMGVHADHYRLELFGADGTIIGDGDLRSAEYLS
                     GLATDDGLSPLAVNEREPAHPEHLPKGLAGHAARAMALMLQDWLPAFDGKDSAAATFD
                     DGLLSLAVIDAAQRSAQGGGWERVHATV"
     gene            complement(42377..43510)
                     /locus_tag="VGP27_26595"
     CDS             complement(42377..43510)
                     /locus_tag="VGP27_26595"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEV7854905.1"
                     /translation="MQTQAAVLWERNSPWSVETIDLDPPKAQEVLVELHASGMCHSDD
                     HLVTGDMPIRLPCVGGHEGAGVVKAVGDHVSWLAPGDHVVFSFIPSCGRCRSCSTGHQ
                     SLCDLGAKIYSGMQIYDETARHHAAGQDLALACGVGSFAHHTVVHEASCVKIPEHYRL
                     DLACLLGCGFITGWGSAVYAADVRPGDTVVVAGVGGIGAAAVQGARLAGARTITVIDP
                     SEYKREEARKMGATHTAADWTEAKGVVAEATWDRGADRFICAMGVGDGQLIGQALAMT
                     AKRGRVVVTNIHPMLERDIRANLMDLTLTEKQIVGTLYGSGNPRADIPKILELASAGQ
                     VDLESMVTRTYPLEKVNDGYADMHSGANIRGVLIYPAAEESGS"
     gene            complement(43510..44994)
                     /locus_tag="VGP27_26600"
     CDS             complement(43510..44994)
                     /locus_tag="VGP27_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7854906.1"
                     /translation="MTFLPESAVMLEFTASYVAGKWTQETGAEGRLQVWSPASGELLG
                     TVGNAGQAEVDAAVAAARAALASPPWRDASVTDRAAAIGRLADALFARKGLLADLTTA
                     EIGSPRSWSTFGQVLTAVGVLRAYAAITPDYVFESTRPAFGGGTVRVRQLPVGVVGAI
                     IPWNTPLFIAALKLGPALAAGCTVVLKPAPDAPLSIAVLTEAIEEAGVPDGVVNVVNG
                     GAATGEALTVHPDVAKITFTGSTAVGEQIAASCGSRIRRCSTELGGKSAAIVLPDAPL
                     ESTVAGLVTGVMGNNGQLCAALTRILLPSSRYDEFVTALTAAVADLVVGDPADRSTQI
                     GPVINETALDRIDGFLQRARDQGATVLTGGDRVDGPGWFVSPAVVSATNDMEIAREEV
                     FGPVAVVISYDDSVDDAAAVAIANDSRYGLVGAVWSADDDRAADVASRLQVGSVAINS
                     TAVLDFGSPFGGFKMSGIGREGGPEGIAGFVEYQAIVRPPRKDS"
     gene            45073..45510
                     /locus_tag="VGP27_26605"
     CDS             45073..45510
                     /locus_tag="VGP27_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005573072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7854907.1"
                     /translation="MSSSPDAGDGPVAGSRWEVMVERGKIAEFADAMGSDNPAYRGPD
                     AMIPPTFLTTAARWAPPGSRVDVGFDRKRLLHGEQEYVFHTPPPRSGDVLTAHERIVD
                     RFSKPGKRGGTMRFATVLTEYRSSDGTLVAEAKATFIETAARR"
     gene            45507..45989
                     /locus_tag="VGP27_26610"
     CDS             45507..45989
                     /locus_tag="VGP27_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV7854908.1"
                     /translation="MSPGEEHGTQTAPALVRPGVEAPPREFGPLTRQMFVRYAGASGD
                     LNPMHYDDELARSAGYPSVFGQGMLTASLLATFASDWLGADTVRRFAVRFREQVWPDD
                     VLTCAGTVASVVSDGDADRVTVELAVRRQTGGVAVTGTAEFLISRNAAPRGADRAFRP
                     "
     gene            46002..47342
                     /locus_tag="VGP27_26615"
     CDS             46002..47342
                     /locus_tag="VGP27_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854909.1"
                     /translation="MSEASIVRRSSYGPSSPAVGARGASTRTRITEVSLELFGRLGYF
                     DTSVDAIAKAAGVSRATLYQYFHGKDEIFLELLNECGSALFRVARRIGPLGPDEVGFD
                     NLNWWLGEWSWVFEKYSTMFVQWTAIATSDTKVRPQITSFVRSYNHRVAERLAASGVS
                     GLDPEAAAMMMTALVHRINLFVHTDRAYGRSAKSAVDTLSVFLQLALFPDTPPSVLTS
                     LRLHASEDPAADVDAIEVPPAPDIEGLSISDRTANLSKRAVNTVTALADAGASQFRAR
                     GYRSTSVDDIVEAADVARGTFYKYFSDKQDLLAAVGAEIYEAGKAFAERIARVDPLDA
                     EPTLRNWVATYVEFYDRYSGCIDAWAEGTADNPVIVAIGENGQVLMDIGAARMLISRP
                     GPYPFDPIVAALIVRALVTRVPQAALDLPEPLDDDSVVDLLISCIKRGFFGRAS"
     gene            complement(47311..48522)
                     /locus_tag="VGP27_26620"
     CDS             complement(47311..48522)
                     /locus_tag="VGP27_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV7854910.1"
                     /translation="MSAEAPLAGVTVVSLEQAVAAPYATRQLADLGARVVKVERPDGG
                     DFARGYDRAVLGDSSYFVWLNRGKESLTLDLKHPDGRRVIHRLLERADVLVQNLGPGA
                     AARLGLDAAAMAERNPRLIVATITGYGDDGPWQDRKAYDLLVQAEAGLLSVTGSAADV
                     ARAGVSIADIAAGMFTFSGILGALYVRATTGEVRSVSVSLLDALVEWMAQPIMYGHYG
                     GSAPRRTGARHPTIAPYGPFTAGDGGSVLLAVQNQPEWRRLCEEVLDRPELVRDPRFV
                     DNADRVAHRDELESIITAAIAELTTADFAQRLEDAGVANARMNDVEQVWNHPVLVERN
                     RLREFRTPGGVAMAHLPPTAIAGIDPSLGAVPALGAHSANILCDLGYSPQQIDELTSS
                     GVTTLGRRTHA"
     gene            complement(48564..49430)
                     /locus_tag="VGP27_26625"
     CDS             complement(48564..49430)
                     /locus_tag="VGP27_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HEV7854911.1"
                     /translation="MASDRLVVCASHSPGKERDVEHAFGQKFRAALAQAADEVHGFDP
                     DVVIVFGGDHRRAFRHVVPTFGVALSASIMAEGGHPAGDMAVPSDLARGLCEHLLAED
                     FDVAVCRDIELDHAFAQPIRDLLGALDAKPVIPVPVNCATAPLPTGRRVAEFGAAVAG
                     FLATLDLRVLLIGTGGLSHSPPSLEVDAYNLSDDERARLISEGMAEARNKIRPDWDAE
                     VLQAMSAWDVATLIRLVDSAHTRAGAGANEIRTWVAAGAAGGGRPVTPVAYEPVPEWV
                     TGMAVATSDPVR"
     gene            49520..50818
                     /locus_tag="VGP27_26630"
     CDS             49520..50818
                     /locus_tag="VGP27_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854912.1"
                     /translation="MKLPSMGRRPVKRRWAAAIGYSFGLVATVNGYRPLARRGFPSVF
                     AFLAGVVATELPLQMIAAQAAGLACVSRWLPRRARRVAVLTAAMSWMGLLGLRRIGHR
                     ADTPLTEALDIALGTDRRTESAGLWRHPDRLDGTAKQPGLVRMLRIYRDYAHDADIPY
                     GDHGAANRLDIWRRRDLDKAGRAPVLIQVPGGAWATGNKRGQAHPLMSHLTELGWICV
                     SINYRLSPRNTWPDHIVDVKRAIAWVKEHVGEYGGDPNFVAITGGSAGGHLSALSAVT
                     ANDATLQPGFEDADTAVQAAIPYYGVYDFTRTDRALHPMMVPFLERLVVKQPRRDVPA
                     VFATASPITHADSDAPPFFVLHGRNDSFIPVEQARGFVARLREVSTQPVAYAELPFAQ
                     HAFDTFGSARAAHAAVAVEQFLAEIYARRTTDVAPRALAL"
     gene            complement(50815..51009)
                     /locus_tag="VGP27_26635"
     CDS             complement(50815..51009)
                     /locus_tag="VGP27_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV7854913.1"
                     /translation="MKAAIDAEKCMGHGMCYSLAPNVFEDDDQGYGHVIGSGEVPQGQ
                     AEQAHNGAANCPEGAITIED"
     gene            complement(51006..52199)
                     /locus_tag="VGP27_26640"
     CDS             complement(51006..52199)
                     /locus_tag="VGP27_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854914.1"
                     /translation="MTTDALPKVSTDALPMPEARDDAWHQMGEHPFVEVEDGYAATHR
                     DVVELILKNPSAFSSKRAFDVLASPVPLVPIAFDPPEQTRYRRILQPFFSPRVIKPLE
                     PDLRQQVRDLIEPIAQRGECDFIAEVAAVFPIQVFLTLFGLPLDMRDQFIEWKDAVLN
                     LSAAAGQTSVDEAAQEGMLKALELFTYLSELIQKRRGAPGDDVLSQVLSIEPPDALSD
                     EEAIGLCFLFVLAGLDTVMDSLGFGMQRLAENPDKRREIVNDPTLVPAAMEELLRLDP
                     PAPFIPRVSTEEVTVAGHTLPEGSRITSYLAVANRDETMYPNPYEIDFHRSENRHMSF
                     GMGVHRCLGSHLARLEMQVLYEEWHKRIPNYHITPGTTPRVHWPRGTVGLDSLHLTLE
                     TGGRE"
     gene            52693..53523
                     /locus_tag="VGP27_26645"
     CDS             52693..53523
                     /locus_tag="VGP27_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEV7854915.1"
                     /translation="MTVFQIACPILALVAIVYVIRKSVRERQLCVEAAVVIGSAIAWW
                     HDPLINWFQPVLFYNAGLVNFSNWMENVPGSLSPGSRFMAEPVLMIGMIYIWMPLAMG
                     RVAKWAMGRARRQWPTLGPVRTFCCGWLAVYVIEFPLEIFAVHHGLVAYPASIPGVTL
                     WAGQTVQIPLYGPILWSLVLSSSGALMFFRNQKGQIRVEAGVETLRRAGPRVRGLLRV
                     LAVTGFLHVVAIGMYDLPVNLAGLYAGPTDTYPTYLRTQYCGPDTPRACPDGALFDDP
                     "
     gene            complement(53529..54119)
                     /locus_tag="VGP27_26650"
     CDS             complement(53529..54119)
                     /locus_tag="VGP27_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7854916.1"
                     /translation="MARGDRSPRSEHADKMRTALLEAALNLFSANGYEQTTTDEIADA
                     AGVSPRTFFRYFPTKESVLFSGEYGFINAFSGVFLAQDETLSDYEAMADSFALLAPGL
                     KRIRKRIAQYHEAVASSFVLQGRERQNHQANADTVANVIAERRRLASPDDECRLLAAI
                     GMLLVERAMIRWLSVANRALDDLIRQEFAALPSVLK"
     gene            54285..55463
                     /locus_tag="VGP27_26655"
     CDS             54285..55463
                     /locus_tag="VGP27_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854917.1"
                     /translation="MADTALNGTANINDLPFATDRSRAWRELREAGEAVSSGEEVVLT
                     SASAVEFAAKKPEIFSSARAFDRLGSPVPLVPIAIDPPDHTRFRRMLDPFFSPKKMAE
                     REPEFRRQAGELIDAIVARGECDVVADLATPFPSQVFLTLFGLPMSDRDRLVKWKDSI
                     LQFTDPSSAEATPEVLTHAMELFTYLTEHIAERRNNTTGSDMLTQLMQNTEDGGMSDN
                     EILGLCFMFVLAGLDTVTSAVGFALAKLAGDSDLRHRISNDYSLIPAFIEDLLRVDGP
                     VPFAPRVTTEDVEVAGRLVPKDTTVMLSYGSADRDPQRYPDADNVHLDSKAVHFAFGR
                     GPHRCLGSHLARLELRLILEEWHTRIPEYTLADGDEPQMPWPTGTMGLKSVPLKFSAS
                     "
     gene            complement(55473..55673)
                     /locus_tag="VGP27_26660"
                     /pseudo
     CDS             complement(55473..55673)
                     /locus_tag="VGP27_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(55916..56341)
                     /locus_tag="VGP27_26665"
     CDS             complement(55916..56341)
                     /locus_tag="VGP27_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854918.1"
                     /translation="MDDYEEIRRVVALHAQLLDERRWTELAALFCTDGVLPWGGKTYR
                     GRKEIIEGLPGTQPEIPHRIKHFVYAPVIEIDGDEARAWSDVIVSLVPEAGPAEMSFV
                     GRYHDHLCRQNGRWLLFKHITVKTGDPLPDDERLPNATD"
     gene            complement(56547..56957)
                     /locus_tag="VGP27_26670"
     CDS             complement(56547..56957)
                     /locus_tag="VGP27_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854919.1"
                     /translation="MNRIRLVMIALFTGIFAIALAPSAYAQDTVTYEVVSNDVAEATV
                     EYFDSSERKVLSAVPLPWRTSVTVINARDPSTDGAEVRADWRPRVCDGRARCPEARPN
                     NWVTVRIYLGGKVICESTLDVGNAACYGSTSFKS"
     gene            complement(57001..57429)
                     /locus_tag="VGP27_26675"
     CDS             complement(57001..57429)
                     /locus_tag="VGP27_26675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854920.1"
                     /translation="MCALLVSGGMVLVPSALADSVTRVQDAMATARAGTSCQPLNYNP
                     ILEKAAGIINQSTDDYIDHLSTYQPITDPMPGLKDLGYGGNKAALLQGALSNEADAIK
                     GALIQGHAAIPDCSYTDLGIDVRRNEETDNLLVVYLLAGA"
BASE COUNT         9763 a        18787 c        18804 g        10272 t
ORIGIN      
        1 cacctgatgc acaacctgcc ctcggagcgg ctgtccatcg caatctctgc gattgcaggc
       61 gcgcgcgaga cctggcgcca gacattgcag tatgccaagg accgcaaggc ctttgggcaa
      121 ccgatcggaa gcttccagca caaccgcttc ctgctggccg agatggatac cgaactcgag
      181 atcggtgagc agtacatcga ccgctgccta caagctgtgg tcgacggcga gctgacggcg
      241 gtcgaggcgt cgaaggccaa gtggtggtgc acagagaccg ccaagaaggt gatcgacggg
      301 tgcgtgcagt tgcacggcgg ctacggctac atgacggagt atcgggtgac ccgcgattac
      361 atggacaacc gcatcatgac gatcttcggt ggcacaaccg agatcatgaa ggacatcatc
      421 ggccgcgacc tcggcctatg accggctcgc cggtgtggcc tatgaccggc tcgccggtgt
      481 ggcctatgac cggcccgccg gtgcggagta tgacccggcc gtggtgaacg gcatctcgac
      541 cagtcgcgac ggcggggtcc tgcgggtcct gctggatcgc cccgacaagc tcaacgctgt
      601 caacaccccg atgctcatcg agcttgtggc ccggctggct gacgcggggg ccgacgaatc
      661 ggttcgtgcc gtgctgctga caggagccgg gcgcgcgttc tgttcgggcg gcgatctgtc
      721 cggcgccgac accagggggg ccgcgcaggc ggccaacgag gtgatccggg tgatcaccgc
      781 attgccgaag ccggttgtcg ccggcgtgca cggccctgcc gtggggttcg gctgtccgct
      841 cgcgctggcc tgcgaccttg tggtggtggc ccgttcggcg ttcttccaac tcgcattcag
      901 ccaggttggc ctgatgcccg atggcggtac gtcggcactg ctgcctgccg cgatcgggcg
      961 tgcccgggcc gcgcggatgg ccctgaccgc cgaaaagatc tcggcggcaa cggcattcga
     1021 gtgggggatg gtatcgcacg tggtcgctga cgcagccttc gagacggagg tgaccgaggt
     1081 gacgcgcgac gtggccgtcg ggccgacgca gtcgtaccgg tggataaagc gcgcgttggc
     1141 ggccgcgacg ttgtccgaac tcgagtcggt gcaggcgatc gaggccgacg gccaacagaa
     1201 actcgcgaag accagcgact ttcgacgggg caccgtcgcg ttccgcgagc gtcgtcggcc
     1261 ggaattcaag ggcgattgac gaaggagttc gcatgcagaa gccgatgacc ggtgtgcgcg
     1321 tcctcgaggt cgcgcagttc accttcgttc cttcggccgg cgcggtgctc gccgattggg
     1381 gcgccgacgt catcaagatc gagaatccgg tcaccggaga cgcgcaacgt ggattggtcg
     1441 ccgtcgatgg gcgacccgcc atgcacgagc gcacctcgtt tgcccccgtc gtcgaggcgc
     1501 ccaaccgggg caagcgcagc atcggccttt cgcttgcgct gaaccagtcg cggcgggcct
     1561 tctgcgaact cgtcaaacgc agcgatgtgt ttctcaccag ccaccttcca cagattcggg
     1621 cgaagctccg aatcgatgtc gccgacgtcc gtcgcatcaa tcccgacatc atctacgtcg
     1681 caggcagcgg ctacgggagc gcgggacccg accgggacac gggtgcctac gacgcgactg
     1741 cgttctgggc ccgtggcggt agctccgacg gcgtcacacc agccgacgcc cgagcgccgg
     1801 cgaacatgcc tgcgggggcc ttcggggaca acatcggcgg catcaccatc gccggtgcgg
     1861 tagcggcggc gctgtacgga cggcgcacga cgggcgaacc ctcggtcatc gacgtgtcac
     1921 tgcttgcgat cggcgcgtgg acgacgcagt tcagcgtgaa ccgcgcaatg cagtacggct
     1981 gctcactgcc gaagatcgac cgccggacgc aggcgccggg caacccgttg acgggggcct
     2041 accagtgcgc cgacggccgg ttcgtccagt tgtcgatgtt gcagccggcc cgttactggc
     2101 cggagttctg ccatctcttc gggttcgacg aggcagcggc ggacgaccgg ttcacatcgc
     2161 tggagtcgct ggcggccaac gccgacgagg ccacggcctt ggtcgccgac gccatcggtt
     2221 ctcgtaactt cgatgagtgc aaggcacttc tcaaccagtc gggcggccag tgggcgcccg
     2281 tgcaggacgc ctgggacgtc gccaacgacc agtcgctgat cgccaacggt cgcatcgcca
     2341 acgtcgttga cgcgcagggc aacgcgcaga aactggtggc gaacccggtg aagttcgacg
     2401 acgcgtcgcc gaacctgacc cgcgccccgc agttcgccga acacaccgac gatgttcttc
     2461 gtgaactggg catcggcgag gacgaactac tcgacctcaa gttcgacggc gcgatcacct
     2521 gacaccgccc cgatgacgtg gttggagcat atcgacgccg ttcatcgcgg caatgattgg
     2581 cctgcggtat tcgatgcgac aggaccggta agcggacgcg ccctgatcgg caaggcggtc
     2641 tatgccgcgg agttactcac cactctcgaa tctcgaccgg gccgggcagt gcccgccctg
     2701 ctcactgtca acgccgatgc cctggcgctc cttcttggcg gggcggccgc aaatcgaccg
     2761 ctggcgccgc tcggcccgag gctcacccag gccgaactgg ccgccgtcct gttcgcaacc
     2821 gacgccaggg tgttgttgac ggagcccgcc ttcgccgaaa ccgcgcgtgc cgctgcccaa
     2881 ctggccaacg tccgcgtcgt gtcgctcgaa tcgctgccct cctccgtcgg ccgactggat
     2941 ggcaactgcg aggtggcgtt ccatctgcac acggcgggca ccaccggtgc gccgaagccg
     3001 gtggctttca ccccttccgt tctcatcgcg cgcggtgagg tgctcaccga actggtcggg
     3061 ttcggggccg gggaccgcta cgcgaccggt tcgccgatac atcacatcgg cggtctgggg
     3121 aacgtcctca cggcactggg cgccggggcg gccatcctgc ccaccaccta tttctccgct
     3181 gcctggtggc gcagccttcg tgagttcggg gcgactcact gccttttggt gccgagcatg
     3241 atcgaaatgc tgctgcggga cggacttctc gatgccgttg cgctgcgcac cctgatctac
     3301 ggcgcttcgc cgatcaccga gtacaccctg agccgggtac ttcacaccct ccctggcgtg
     3361 cgagtcgtca gcctgttcgg tcagaccgaa ggcagtccga tcaccagcct cggcccagat
     3421 gaccatcggc gcgcgactga aggcgcgccc cacctgctcg gtacggtcgg gcgcgcggtt
     3481 ccgggattgc gcctcaccat cgacagtccg gacgaaaccg gaaccggaga ggtgctcgcc
     3541 gcggcatcgc atttgtcggt ctgcggtgcg gatggctggc tccacaccgg cgacctcggc
     3601 gtcatcgatg ccgacggcta tctgcggctg gtcgggcgac aacacgacat ggtggtccgc
     3661 gggggtgaaa acgtctaccc ggtagaagtc gagaacgtgc tgtgttcaca tccccgcgtc
     3721 gcggcggcgg cggtgatcgg cgtggccgat tcgcggctgg gcgagaccct ggcggcgtac
     3781 atcgtccccc tcgaccgaca tgaccctccc gatgagaatg acctccggtc tttcacacgc
     3841 gcgcggcttt cggggttcaa ggtgcctgcc acctggtcgc tgctcgatca attgccgcgc
     3901 aacagtgcag gcaagctgaa ccggctggcg ttgcagcaga tgcacgatcg agtcggaggc
     3961 taacccgggt gctcccatcg acggcaccgc tcgatcagaa tgcgcgccaa cagttctgga
     4021 tggctgatca tcacctcgtg gcacgcgtcg accggaatca cggtctgcac cccaccgagt
     4081 gcggcgatgc tgtcgcgctg tgaggagacc gacagcgccc gatcgcgggt tgtcagaatc
     4141 caggtcctcg ggacttcgtc gggcagtccg ctgcgatcga cgccttcggc gggaatccgc
     4201 gccgactccg ggtgaagacg cgacagcgtg aatcggcgct gctcacgcgt cataccgtta
     4261 cagaacgcat accgtgcggc cagctcggga accttcatcg gattgccaat tctcgccgcg
     4321 cgccgggcga accaggccaa cgggccaccg agcgtgtcca cgatcgcacc gccctgcggc
     4381 ggtacgaacg cggtcgccag aatcatctct cgcacccggg ccgagccgag tttggtgacg
     4441 acacccggca ccgtcacacc ggccatcgaa tggccgacga tcacgatgtc gccgaggccg
     4501 gcgtcctcga tgtccgcgac gaccgaatcc acccactccc caatggtgac ggtggcgagg
     4561 ttccccggct tggcgccgtg gccgggaaga tccaccgcga gcgtccgcaa ttccggagcc
     4621 tgtctgcgca attcgacaag ggtcggttcc cagcagtcac ccgcgtgctc gccgccgtgg
     4681 acgaggacga ggtcgggcag tgccatgtcg gcgaatcctt ccggttagcg gtgcacgagt
     4741 tgggacgcca gcatgtcgat gtcggcgcgg aaatcggcga ggctcctgct ggccggctcg
     4801 atcgtgatcc aggtgacgcc tgcttcggcg tacgcgtcga gctgcggcgt cacctcagag
     4861 cagaacgccg tgctgtcacc gctcgtcagc agttcggcct cgaacgggac aaacgacacg
     4921 tcggcggcgc aacgaccgaa ctctgcgcgc cgcttgttga tgccggcgat ccagtcggcc
     4981 agtgtggcaa tgtcttccag cggtcgcgcg cgcgtgatcg ccgccatctc accggacgcg
     5041 gccatcggca tccagccgtc ggccacttcg acgatccggc gctgcgcggc gttactgttc
     5101 ccgccgatcc agatcggcgg cccgccctcg gtccgtggag ggggcaaggc gaggtggcct
     5161 tgcactccga aatccggtcc gtcgtggtcg cgacaggccc atgtcgcccg ccacgcctcg
     5221 atcgcctcgt cgagtaacgc gccgcggcgg ccgaagtcgg cgcccagcgc ctcgaactcg
     5281 gacttgagat agccggcgcc ggtgcccaac gtgaagcgtc cgcccgacaa cagatcaagg
     5341 ctggcggccg ccttggcggt cagatacgga ttgcggtaac ccgagaccag aatgtaggtc
     5401 atcacctgaa tgcgcgtcgt cgcggccgcg gcgaaactca gggacacgaa cgggtcgaac
     5461 gcgtggtggc caccgttggc cagccagccc ttgtcgggat aagggtgttc cgacatcgag
     5521 aatccgtcaa aaccggcctc ttcgaccagt ttcgcgacgt caccgatcga ggcgcccccg
     5581 caccagtcat tccagtgctt gactgcgcgc atcgggaaca tcaagttgta gtgcatcgca
     5641 ctcctatctc accgtgcctg tttcaacaac gacctggcga tcacttcgcg ctggaattcg
     5701 gtggcccctt cgccgagccg cttcaccctc aggtcgcgga accagcgctc cagcggcaat
     5761 tcgtcggaaa gcccgagcgc gccgtggatc tggatgcacc ggtcaagcac cttatacgcg
     5821 gcttcggtac cgaacatctt ggccaccgac gcgtcgacgc ggacgtcccg attcagatcg
     5881 gcattccagg ccgcctgata catcagtagc cgggccgctc gcagctccac ttcgctgtcg
     5941 accagcatcc attggatgcc ctgtttgtcg gcaagcttgc cgccgaacac gtcgcggtcc
     6001 tgcgcccacg cgcacgccat ctccagtgcg gactgcgcaa tgccgatcgg ccccgctgcg
     6061 tagatgattc gcccatgcac caggaaatcg ccggccaggc tgaggccgtg gccctcctcg
     6121 ccgatcagct gttcctcggg aatcctgaca ttgtcgaagt gcagctcgta aggagcgaat
     6181 gacgccatca cgccgatgcg gctgaatatc acacccggcg tgttcttttc gaggatgaat
     6241 gccgaaatgc cggtccggcc ttcaccggta cgcgcgtaca cgacgcccca gtcggcatcg
     6301 ggcgcatgtg agatccacat cttggagccg ttgagcacgt aggtgtcacc gtcgcggatt
     6361 gccctgagct ggatcgcccg cacggggtcc gatccgccgg aggcctcggt gatcgcagtg
     6421 tatgccttcg tcatggtgcc atcgatgatg ggccgggcgt acctcgcgaa ctgctggggt
     6481 gacgccttga acatgatgtt cggcgggttg ccgccgaatg cgccgagcgc cggaaagaag
     6541 gctcccatgc ggcatttcgc cgcctcctcg gcgacgacca cctgcccgag tacgctgagc
     6601 ccggcacccc cgtgctcctc gggtgtctgg agtgcccaca gtccgagcgc acgggccttg
     6661 tcctgcagtt cgaccaggcg ctcgcgcggc agcccgatcg cgtcgtggtc gagtttctcc
     6721 tcgatggggt gcacgtgggc atccatgaag cgccgcacgg tgtcccgcag catcaccaac
     6781 tcgtctggca gctcccatgc accggtttgg ccggcaggcg agggcatgtc agacgcggga
     6841 cgtcgcggcc gcccgcagat cccgcgcgct ctgcaactgc ggctcgtggt tggcctcgta
     6901 ccgctcgatc cattcgtgcg gcagcttgcc ccaggcctcg ccgatccgca gccgctgtcc
     6961 ggagctgaac acctcggcgg ccaccacatc gccgacgaag atggtgtttt cgtcattgtc
     7021 cagcgagccg gtgacgcggc attcgacgta gctgtgcgcg tcgaggagta ccggcgcccc
     7081 tgtgacgccc gccttggtgc gcagccgcga catcttgtcg ccgtcacgac cgctgctgcc
     7141 gccgagggtc atcaggatct ccaacgactt ttctacctcg ccaggatccg cggacagcat
     7201 gtgcatcacg aaaatgcccg agttgatcag catgtcatgc gtgcggttgt atttcgtcag
     7261 gctgacggtg gcgcgcggca gctccggaac gatgccggcc gcgcctgccg acaacgagat
     7321 cagtccgttg gcgaagccgt cgtcgatggt ggtgacggcg acggggaagg gtctcagtcc
     7381 ggcgaggact gcattggctg cctcgtggtc gatggtcata ttgttcctcg cgtccgcggt
     7441 ggtcatcggt tcctcctgtt gttatccgag cgtgaagggg tcccgggttg cccctgagag
     7501 ctccacccac accgctttgg tctcggtgaa gtccttgacg gcgttgatgc cgttctcccg
     7561 gccgatgccg ctgtgcccca tgccgccgaa cggaacgtgc ggcgccacaa cgcgataagc
     7621 gttgacccac acagttccgg tgttcaattt cgccgcgacg cggtgcgcgc ggtggacgtc
     7681 ctttgtccac accgatcccg caaggccgaa ttcggtggcg ttcgccgcgg cgatggcctc
     7741 gtcctcgtcg ctgaacgtca tgaccgccag caccgggccg aagatctcct cggccaccgc
     7801 ccgcatcgac ggatccacgt cagtcagcac agttggcttg acgaagaacc cgccgagctc
     7861 accggcgggt tcgcccccgt aggccaccgt cgcgccctgc tcgcgcgcgg atgcgaagtg
     7921 tgacaacacc ttttcgtatt gaggctggtt ggacatcggc cccatctcgg ttgacgggtc
     7981 cttgggatcg cctagcttga tcgtcgccgc cctggtaacg atgcgctcca ccaatgcgtc
     8041 ggcgacgctc tcgtgcacca gaagtcgtga gcccgcaagg cacgtctggc cggtagccgc
     8101 gaacacaccc gcgatcacgc cgttggccgc cgcgtcgaga tcggcgtcag cgaataccac
     8161 ctgcgccgac ttaccgccga gttccagcga gaagcgggtc atattttcga tcgcggcctt
     8221 gcccaccgag atcccggtcg ctgtggaacc cgtgaacgcg accttgtcca catcgggatg
     8281 cgaggccagt gctgctcccg tttccggtcc ccatccggtg acgacgttga tcacgcctgg
     8341 cgggaaaccc gcctcggcga acaactttgc gaaggcgagt gtcgacgtcg gtgtgtggtc
     8401 gctcggcttg accacaaagg tgcaaccggc agccaggccc gcggccagct tccacgtcaa
     8461 cagcagaagc ggcgagttcc acggcgtgat cgcccccacc acgccgaccg gctcatgccg
     8521 ggtgtagacg aggtagttcg gcttgtctgt gggcagcacc tcgccctgca gtttgtcggc
     8581 cagccccgcg tagtagaagt agtagtccgg cagggaccgc atctggccga ccatctcgcg
     8641 gacgagcttg ccgccgtcac ggacttcgag gccggccaga ttctccgctt cccgcgcgat
     8701 gatctcgccg agccgccaca gcagcttgcc gcgatcggtc gctgtgagct gaccccacgg
     8761 cccgttcagc gcggaccgcg cggcagcaac cgctcgatcg acgtcggccg ccgcgccgtc
     8821 ggggacgcgg gcccaaggcc gtccggtgta cgggtcgacg ctgtcgtagg tggcgcccga
     8881 ggccgcggcc gtcggttcac cgccgatgag cagttcgaac tcgaccagtc gctgggtagc
     8941 ggtgtcagtc atgcgatctc ctcatcgagt ccgcggcttc ttcttgttgg cgcgggaatc
     9001 ggtccggcct gcgcccgggt tgcccagatc tgtgtagaac ttgccgtgcc gcgcagcggt
     9061 gctcggtgcc aggtcgcggg cttcccacat ggcgcggacg gtgccctgaa cgctcagggg
     9121 gcgccgtgct gcgatctcgc acgccaattc ggttgctcgt gaacgcaatt gatcgtcggg
     9181 aacgacctcg gtcacgatgc ctatccgcag agctgtctgc gcggaaatcc gttcctcact
     9241 gccgagcagc gcccaccgca tcacctcgcc gtagggcaca ccgagcgcga gcatgcccat
     9301 cggttccagg gcggagacga tcccggcatt ggcgtgcgga tcgaaaaagg ttgccgtctc
     9361 gctacagatg gcgaaatcgc attcgttgac gaagtacatc gccccgccgg ccacgatgcc
     9421 gtgcagcgcc gcgatcaccg gcttccatac ccggtgcgct ttcggcccca gcagctcgcc
     9481 tgggtcttct tggttgaaga tcggcttgtg cgtccaccag gttccttcgg acacgtcgat
     9541 cccggtgcag aaggctcggt cgccgttcgc gcgcagcacg gccacccgga tgtcgtcatc
     9601 gtcgcggacc tttgcccaca ccgcggacag ctcgtcggcc atctcctctg tgaagctgtt
     9661 cagcttctcc ggcctgttca gtgccaccgt cgcgacgtgc tcgctcacgg cgaaatcgat
     9721 tgtgcgcagg ttctccaggt cagtcacggg ccgatcctac atgtgtaacc tggttagcta
     9781 tctcgcgttt gaggatcttc cccgcaggtc ccagcgggaa ctcgtcgcga tactcgaatc
     9841 ggcgcggctg cttgtaaccg gccaactcgg ccctgcacaa cgccgtgagt tcggcggtca
     9901 gcgcggcacg gtcgttgacg tgggacttcg ggatgacaac ggctacgggg gtctcgcccc
     9961 agtcgggatc cggcaccccg acgaccgcga ccaggtccac gcccgtgttc tgcgcgatga
    10021 cccgctcgat ctcggcgggg tagacgttga aaccgccgga gatgatcatg tcggtcttgc
    10081 ggcccgtgat gtgcagatag ttctcgtcgt cgaggtagcc gaggtcgccg gtgagcagcc
    10141 cgtccgaccg gaacgtctcc gcggtctggg tcggacggtt ccagtagccg accgcgttgg
    10201 cggggctgac aatgacgatc tcgccgacct cgccgccggg tacgtcgttt ccgttgccgt
    10261 ccaccacggt gatgcggcac atcggcgtct ccttgccgca cgacgtggcg atcgacgttt
    10321 tgcctgcaac gatgtcgcgg tgatcctgtg gggtcaggat ggtggcctgc ccgccgcatt
    10381 cggtgagacc gtatcgctgc tgcaaatcac atccgagcag atccatcgcc ttgcgggcca
    10441 ggccgggggg caccggcgcc gatccgtagg agatgcggcg caggctggac atgtcgcggt
    10501 gcgggccgtt ctccaggatg gccagcaccc gatgcagcat tgtgggaatg aacgtggtga
    10561 aagtgattga gctgcgctcg atctcgtcta tcaccgcgtg cggatcgaaa cgctggtgaa
    10621 tcaccattgt ctggccgagg tatagccagg acaccgtgcg gaccatgccg cccgcggtga
    10681 agaacggcgt ggtggccagc atcacgtcgg accggtcggc ctcggtcacg aggttggtgt
    10741 ccgctgcctg gtagagcagc ccgcgatggg tgtgcatgac gcccttggcc cggccggtgg
    10801 tgccgctggt gtagaggacg accgcgacgt catcgggggt ggccgcaacc gacggggcga
    10861 cgggttcggc cgttgagagg acggattcgt actctggacc gatcgtcagc ttgaccgcca
    10921 gtccgtcgac ttgcgcggcg aaagtctggt actggtccgc gtggatgatc gccgcgcggg
    10981 ctcccgagtc gtcgcgcacg tgcgcaacct cttcgggcgc gaggcggata ttgaccggta
    11041 ccgcaatgag gcccgccttc gccagcccga acgagatctc cggccattca atgcaattgc
    11101 gcgcgatcac catcacccgg tccccgggta ccagcgcggt gcctgcgaga tggtttgcga
    11161 gccgattcga ccgctcgtcg acttgcgacc aggtacgggt ccggcctgcc tcgacgagcg
    11221 cccgcttgcc gggatagcgg cgagcgttgt tggtcgagat atctgaaatg agcatcatca
    11281 gatgagaaac gccaaggtat cgatcgggcc ggctgcgtcg atcagatgaa cgatcttgcg
    11341 gaccagccga aggccgttcg gtgaccgccg aagggtgtgc acgacccggc ccgcccagaa
    11401 ccgctggcgt acccggtatt cgaacagggc gaagttcgac tccaccgtca cggtgtcgtc
    11461 gatctgctcg acgacctcgc tgttggacag cagtctgcgc attaccgacg gcggtgtctg
    11521 ggaatgtcgg ttgccggtgt tcagctgtgc cactcggctt ttgattcgcc tgcggttgtc
    11581 gttgatgtag gccagcgtcg ttcgtgcatc atcgtcgggg tgcatgggaa cgcggtacag
    11641 aacgttctcg tcatcgccgg cccacaacga ttcccattcc gagtagcggc cctcgtcggc
    11701 cagccgggat tccagataga gaaacgacaa gacgtcggca tccggtaacg gacgtaacac
    11761 cggtgtggac tcaggttgag tcttgacggt catcgcctca ggtctttccg ccgccgacga
    11821 ctgcggccca ttgcgagtag aagccccgtt gtggcgtctc ggcgctcttg tcgcggttga
    11881 tgattccggt ctcgtcggtg atgtcgtcgt ccatcccgcg cgccagcatc agccactccg
    11941 gcagttcggc cgcgagcccg gcctggttgc gcatcccgat ctccccgtcg tcggcgatca
    12001 ggaagccggc gggccccatc gcgccctcgg agcgccgcag cgtgcgttcg ttgagatcgt
    12061 catggccggg gatcaagacc gcggtggtat aggcgatcgt gcggtcggga gcttgtggtt
    12121 ctacgaacat cacgttcatc tccgccagaa acaggttcgg ccagatcaaa gtgtgcgggg
    12181 ggccgaccac cagtgcgtcg tgcgcctgat cggggccgta ggccctctcg agggcgtcga
    12241 cataacccgc cagcttggcc cgcgggatac ggccgtacca cacgaattcc tcgtccagct
    12301 ttcgatattc gtcgctgtag tcgatctccg agtggccgcc gccgatgtcg cggaccagaa
    12361 cgtcgacctt ggtcggcaca tgggacacct tcgccggctt gatggcgtca tacaccgaag
    12421 cgtgggtgaa cagcgcgtgg tagccgtcga cgttgttctc caccaccatc ttccagttgg
    12481 cgtggtggag gtgcttcatc cagttggccc gcaggtcgat gctgcggtcg ggggacaggt
    12541 tcaacagccg gtcgatcgcg tgcgtcgccc ctccgaggtg ctcgcgcagc gagatgccgt
    12601 cgggcgccag cgaggcgaac acgaatccgc catagctgtc gatcctgggc gcctgcgcca
    12661 ggcccaggtc ggcgcgcacc tcggcgaacg cctctccgta gccttcgcgc atcggaacac
    12721 cttgcagcgc gccggtgttg ttgaacgtcc agccgtgata agggcagcga aacgagttgg
    12781 cgttgccctt gtcggcattg cacagcttgt tcgcgcggtg tgtgcaacgg ttcagcagca
    12841 cacgcacaac gccgtccttg ccgcgcacca cgacaacggg gtccgacccg atcatccggg
    12901 tgaggtaatc gcccggctcg ctgacttcgc tttcgtgcgc gacgtatacc cagccggtct
    12961 tgaagatggt gtccatctca cggcggaaga tctccggcga ggtgtagacc gagccgtgca
    13021 cgcggtcgct gcgaacgacg gtggccgcat cgaaatcctc ggcggacgtt gttgtctcga
    13081 gagtcgtcac tcggcctcca ggttgactgc ggggatcgcc acgtcgccga ggcggcgaaa
    13141 tgcgatgtgc acggtggtac cgatgcgcgg tgcggtatcg gtcggtgtga gggtggtcat
    13201 gatgatgcgg tgaccgctgt ccatctcgac gtcgacgatg gggtacgggt cggtggcgcg
    13261 caccagtccc gcttcccggc ggtgcatcat cgttgccgcg tgcacgatcc cgcgcgggtg
    13321 cacttgtctc caaacgggtt cggtgcgacc gcaggcatcg caggtctgct gttcgagttc
    13381 cagcgctaat tcgcagcctg cgcagaaggg cagtgcgagg tcgccgcgcg ccgcggcgtc
    13441 gtagagcggg cgaagggcgt cgccgttgat cgcgggcgcc agcgcgcgtt cgagcagcca
    13501 gtcctcgcgg gccgacgtcg tgagggccgt catgccgctc gctccagtat cagcgcggca
    13561 tggtgatcca tccgaccgcc tatcccgccg accagcgcca cggtggcgtc ggggacctga
    13621 cgatctccgc cggacccacg aagctggatc actgcctcag ccaacggtgt catgccctgc
    13681 aggtagaagc cggagagttg gccgccgccg gtgttcaccg gcagcactcc gcctggcccc
    13741 gcccgcccgt cctgcatgaa ggcgcccgcg ggggtatcgc cggtgagccg gtactcgtcg
    13801 agcagggcca gcgtcacaat cgagaacggg tcgtacagct cggcgatgtc gagctcggcg
    13861 cgcgtgatac cggcctcatc gagcgcgtcg tctacggcgc ggccgcctcc gccgaaccac
    13921 gactccgcac cggcgcggcg gcgccgcacg ggatgatcgc gacctgttcc gagaatccgc
    13981 agccgggccg caccaatcga atctcggccc gaaagaacca cggccacagc gccattgacc
    14041 ggtcgtgcgc agtcaagcag acgcaatggt tcggcgatca tcgggctggc ggcataaccg
    14101 tcggagtcga gcggctcgcg actgaccgcg tctgggttgc ccgtcgccca gcgccgagcc
    14161 gtggtcgcca ccgcagccag cgcatcggcg gaggtaccgg tgacgtggag ccaacgctgg
    14221 gccagtaacg cgtaggtcgg gaccgagccc agtaggcccg acgctcgctc cataccgcgg
    14281 acgccgttgt tgccgccgct gtgggcgtac gtcgaacccg accctttatc cgcgacgagt
    14341 ggcgcgtcgg cgaatacgca caccaccgtg gccgcttctg cgtccgcgat ggctttgcgg
    14401 gcgcgctcga ccatcgcgat cgtggtggcg ccctttattt cgacgtgctc caacagccgc
    14461 agattgccga atccaccctg ggcagcgaag cccacgccca accggtcggg tcgcaccccc
    14521 tgcgaggacc cgaccagcag cccgtccaca tcggccagtt cgatgcccgc gtcggcgagc
    14581 gccgaggcgg tcgcctcgca cgccaactgc aggggagtcc cgccgggcac cagtgacatc
    14641 ggtgtcatcc ccatgccgac gatggcgacg ctgtctctgt cgctcattcc ctgggcttcc
    14701 gttcaccgcg gtagttgccg accctcagcc agtcttcccg atcctccgcc gtcgccaccg
    14761 tgaagctgga cagctcgagt gggtcgagga aggcctcgtc gccggtctcg agatcctcga
    14821 ccaacagccg tggaccattg ccgttcctgt cgagccacag tcgcacggcc gcgaactcgt
    14881 tgatcaggat cgccagttcg cggcgcttcg gattgccttt cgatgcgtcc atggcgatgg
    14941 gctttctgcg caggttggct gaatcggtga atacccacac agtattaccg catactagtg
    15001 tcagaatcaa tgtccgaacg aggatcgagt attgcgcgag atagcggtca acatgcagac
    15061 tggctgcatg aacgctggaa gcacagaggc gacacctctg tcagaaatga gtcccaaagg
    15121 agcagcatga ccgacaccgc cgtgcccggc gctgtgatcg acacggatga ctaccgcagc
    15181 atctggatgt acctcaagga gctcgaattc cggcagggct tcactgaggt acgggtcaac
    15241 ggggcggccg tacgcacgcg ctatgccgag gccggctcac cggacaagcc acatattgtg
    15301 ctgctgcacg ggaccggcgg gcactgggag acgtttgcac ccaatctcgc ggcactttcc
    15361 gagcactttc actgcgtggc gatcgacatg atcggcaacg ggttctcgga caagcccgac
    15421 tacgactacg agatcgccgt ctacgtcgaa catgtgctcg gcgtgatgga tcacttcggc
    15481 atggcgcggg cacatttgat cggcatgtcg ctgggcgcgt gggtcgcctc gacgatcgcc
    15541 gtgagccatc ccgatcgcgt tgagaaggtc atcctgatgt cgccggcggg caaggaggcc
    15601 acggcgtcga acatggcaag gatccgtgcc gaacgcacga aggcggtcaa cgaaccgacg
    15661 tgggagtctc ttcacgcggt gttcgcgcat ctgatcgccg aagaggccaa ccggctgccc
    15721 gacctgatcg gcctgcgcca ggccgtctat cagcgcgacg acacccgcaa gacaattgat
    15781 cgtctgttga tcctgcagga cgccgccgcg cgcgaccgca acctcatccc cgaggagaag
    15841 tggcgaggca tcaccgcacc ggtgatgatc gtcgcctcag gcaaagacca cggcgtctat
    15901 caagagacgg ccgcctcgat cgccgggctg atcccggact cggaggtgtt cgagatgccc
    15961 gacgtgcggc attggccgca cttcgaggac cccgaatcgt tcaatcctgc cgctgtctcg
    16021 ttcctgcgcc gatgacgcgg cctgggccgg gggagatcgc cgacatcgct cgtcggctct
    16081 acacagccga gcaggagcgc aagccgatca ggcaactgtc actggactat ccggagatga
    16141 cgatcgacga cgcctacgcg gtccagcgtg agctggtcga cctgaaggtc gccgacggac
    16201 gggtggtgaa gggccgcaag atcggactga cgtcgaaggt catgcagcgc gcggtgtcga
    16261 tcgacgagcc cgactacggg gcgctcttcg atgacatgtt cgtcgacgac ggcggccggg
    16321 tgccgcccgg tcgcttcatt cgtccgcgag tcgaggtgga gttggcgttc gtcctgggcg
    16381 agacgttgcg cgggccggac gtcacgcttt ccgaggtgct gcgcgcgacc gagtccgtca
    16441 ggccggcaat cgagattctc gacgcccggg tacagatgtc cgaccccgag accggtcatc
    16501 tgcgcacgat cgtcgacacc atcgcagaca atgcggccga cgccggactg gtccttggcg
    16561 gacgggagtt tcggccgctc gacgtcgacc tgccgtgggt ggcggcggtg ttgagacgca
    16621 acggcacgat cgaagagtcc ggggtggccg ccgcggtgct cgaccatcct ggcaacggcg
    16681 tcgcctggct ggcgaaccgt cttgccccgc atggtgtttc cttgtgtgcc ggcgaggtga
    16741 tcctgtccgg gtcgttcacc aagccggtgt tcgccgagcc cggcgacatt ttcgttgccg
    16801 actacggccc gcagggcacg gtgtcggtgg ctttcgaccg ggagccacag cggtgacgaa
    16861 ccggtgggcc gagcgtatcg gcgacggccg tccccgattc ggcctgtggg tggcgtcagg
    16921 tagtagctat gtcaccgaga tctgcgcggg ttcgggcatc gactgggtac tgatcgacca
    16981 ggagcatgcc cccaacgatc tgcgcaccac gttggaccag ctgcaggtgc tggccggcta
    17041 tccggacgtc gacgtcgtgg tgcgaccgcc cgcggccgac ccggtctaca tcaagcggct
    17101 gctcgacatc ggcgcacaga acatcatcgt gccgatgatc gacggccccg aggaggcggc
    17161 cgccgccgtc gctgccaccc gctatccgcc cgccggtatc aggggtgtgg gtagcgcgct
    17221 ggcgcgggcg tcgcggtgga atcgcatcag cgactatctc gccggcgctg accaaggcgt
    17281 gtcgttgacg gttcaggtcg agtcgacggc cggcctggcg gcactcggcg atatcgcgga
    17341 cgtcgatggt ctcgacgcgg tgtttatcgg acccgccgac ctggcagcct caatgggtcg
    17401 cctcggtcag cccgagcacc cggaggtggt gagcaccatc gaaaccgcac tgaatgacat
    17461 cgtcgagcat ggcaagagcg cgggggtgaa cgcgttcaac ccgacgatcg cgcagcgcta
    17521 cctggccgcc ggcgccagtt tcgttctcgt cggcgccgac gtggctctgc tggcccgcgg
    17581 cgccgatcag ttggcggccc agtatcgcga tggccgcgaa ccgtaaggac aaagctataa
    17641 ctgtctagct gtttggccgt tattgtttgt gggatggatc ccaatcctga ctacgacgcg
    17701 agcgacgagc tggaatatgg cgtcaactgg ttcgcgtgga tattgcgcgg cgtctatccg
    17761 ccgcccgcat acccgcccgt ttaagacgat cggcccgcat caggcctggc gcactcgtgg
    17821 gccacgcatc ggtggtgcgg tggatcggta gtggtgtccg gtgggtgtgg tcaattcagt
    17881 tgtatggacg cccttctcgt cgtcaccagc aacggcctgc cagcccgatg attctttggt
    17941 gtagttgcag cgttcgcatt tgccagaccg catcttgaca tgaggcaact ggcaaggcct
    18001 gtggatgaat ccagactgcg gatacgtccg tttgaccgtt gactggcagc gtcgcgccga
    18061 ctgtgctcac cggcgtatct gacttgcgcg tcaccatcga caggggaacc actacacgtt
    18121 cctaggttaa attcgatact ggagctttat ttctgactgt tgtgtcactt atactcgggc
    18181 gttatggacc tggagttcac accggatcaa gagcagttcc gcgaccgggt ccgaacttgg
    18241 ctggatgaga acaagccgac cgagccgagg ccccgcgacg acgaaggcat ccgcgagtac
    18301 gacctggcct ggcagcgcac ccagtgggag ggcggctggg cgggcatcgc ctggccgacc
    18361 gcgtacggcg gcaaggggct gaccctgctg cagcagctga tctggtacga ggaatacgcc
    18421 gcacgcggct ttcccgggat cgacgcatgt ttcgtcgggc tttcacacgc cggacccacg
    18481 ttgatcaccc gggccaccga ggatcagaaa tcctttcacc ttccgaagat cctgcgcggc
    18541 gacgtcatct ggtgtcaggg cttctctgaa cccgaagcag gctcggatct ggctgcgctg
    18601 cggaccaagg cggtgatcga cggcgacgaa ttggtcgtgt cggggcagaa gctgtggaca
    18661 agcttcgcca ccgttgccga ctaccaggaa ttgctggtgc gcacggacaa cagcggtagc
    18721 aagcacaccg gaatcacgtg ggtcatttgc gacatgtcga cgccgggaat cgaggtacgc
    18781 cccatcgaga ccattgaagg cggttcagag ttttgcgagg tgttctacga caacgtccgg
    18841 atccccatgt ccaatgtggt gggcgaggtc ggcgaaggct ggtcggtcgc gatggcgacg
    18901 ttgtcgttcg agcggggtac cgcgttcacc gcgaaccagg tgcggctcgc caagattatc
    18961 gaggacctga tcgactacgc ccgtgatcac gtcggccccg acggtcgtcg acctgctatt
    19021 gccgacgatg agatcgccag acgactggca cgggctcgcg cctctgtcgc gtcgctgcgg
    19081 gcgttgacct acaccaacat ctgcgaggcg atgaagaccg agacgcccgg cccgcgtggc
    19141 tcgatcgtga agctgatgta tgccgaactg gccaaggaga tcggcaagct ggcgatggac
    19201 gtcgtcggcc ccgccgcgat ccggcggtcg tcgcggtggg acgcggacgg ctgggcgggc
    19261 tattactact actcgttctc gcaggccatc ggaggcggga catcggaaat ccagcgcaac
    19321 atcatcggtg aacgcgtgct gggtatgccg cgatgacagg agaaggtcat ggatctgctg
    19381 cccgggcctg aacagctcga gctcgtctcg gccgcgggtg agttcctgtc cgaccggatg
    19441 ccgatcgaac gaatccgcgc cgatcgggac gcgcaatggt ctgtgtcgca gcgaatctgg
    19501 cgggagggcg cggaactggg cctgctgacg ctgggcttgc cagaagagtt cggcggctcg
    19561 ggtcggcctc tcggcgatga ggcgctgttg cacatcgaac tgggcaggaa cctggcaccc
    19621 ggaccgttcc tcgccaccac cctcgcggcg cggatcgcag cgaaatgtgg cgatagtgca
    19681 ctcgcggacc agatcggttc ggggaagacc ctcgtttcac tagcggtgtt gcgcggtgac
    19741 ggtgacgtga gaccgatcaa gggcaccttc gacctgttcg accatgtcga tgctcggtat
    19801 gcgctcgtgg tcgcgcgcga gggggcggcg cttgccgaca tcgcctcgtt cggcgaaccg
    19861 gtcgtcatcg aatcggctga tcccggaact cggatggcga cggccactgt gcaatccgcc
    19921 gagccagtgc actggctgtc ggcagacgac gagtggatct ggggtcgcgc agtagcgttg
    19981 tcggccgcct tcctcaccgg ccttgcgagt gccgcaacag agcaggcgac cgaatacgcc
    20041 aagacgcgag tccagttcgg caagcccatc ggggtgcacc aggccatcaa acacacctgc
    20101 gtgaacatgg agatcgccgc gcaggccgct catgctcaga cgttgttcgc ggcgatcgca
    20161 ctggagtccg gccgatccga cgctctgctg caactgcttt cagcggtgac ggttgccggc
    20221 tctgccgccg tggacaacgc cgccgcgggc atccaagtgt tcggcggaat gggatatacc
    20281 ttcgaaaacg acatgcacct ctatctcaag cgtgcccacg tctttcgcca tctcttcagc
    20341 gagccgaccg acgttctcgc cgaactgctg gcgcaggacc gcgcacagtg aacgcaaagg
    20401 tcgccatcgc aggcgtcgcg ttatcggatg tgggccgcgt cgatgacaaa ggtccctatg
    20461 agttgatggc acaggccagt cgcttggcac tggcggacgc cggtttaacg ccagccgatg
    20521 tcgacggatt ggcgtcgacc ggccagggca ccttgcctcc tgtcgatgtg ggtgagtatc
    20581 tggggctacg gccgcggtgg gtggactcga ccgctgtcgg aggtgcctca tgggaggtaa
    20641 tggcctcaca cgcggccgac gcgatcaccg ccggccacgc cgacgtcgtc ctgctgacct
    20701 atggttcgac cgcccgagcc gacctcaaga agggcttgcg tggcgccaac atcaactggg
    20761 gtgcgcgcgg gccgctgcag tgggaggcgc cgtacggcca caccctgatc tcgaagtacg
    20821 ccatggccgc gcgtcggcac atgcaccagt acggcaccac catcgagcag cttgccgaag
    20881 tcgcggtgtc ggcacggttc aacgccgctg acaacccgga cgcttactac cgtgagccga
    20941 tctctatcga cgatgtcctt gacggaccga tgatcgccga cccattcacc aagctgcact
    21001 gctgtattcg cagtgacggc ggtgcggcgg tggtactggt caacgccgac cgggccaggg
    21061 atctgcgatc gcgaccggta tgggtgctcg gcgcggcaga aaccacctca cacatgctga
    21121 cctcgcagtg ggacgacatg acggtcggac ccgccgccgt gagcgggccg ctggcgttcg
    21181 cgcgcgccgg ggtgcggccg gcggacgtcg atgtggccga gttgtacgac gcgttcacct
    21241 acatgctgtt gctcacgctc gaagacctgg gcttctgtcc gaagggggag ggcggccgct
    21301 tcgtcgagaa gggttcgttg cggctcggcg gagagctgcc caccaacacc gacggtggcg
    21361 gcctttcggc atgtcacccg ggtcagcgcg gattgttcct gctcgtcgag gctgctcgtc
    21421 agttgcgggg cgagtgcgga ccgcgacagg tgcccgatgc aaagatcgcc tgcgtcagtg
    21481 gcaccggcgg gtggttctgt tcaagcggca caatggttct cggcgccgag gagccctaga
    21541 tggatcagcc gccgttgtgc gcggatctcg ggaatacccg gtccatgttg gaagcggcag
    21601 cgcaggtgca tggtgaccgg gaagcctatg tcgaaccggg cgcacggatc agtttcgccg
    21661 agtggatcga gcgggctcgc gccgtcgcag cgcaattcct ggagttgggc gttgacaagg
    21721 gtgacgtcgt ggcgctgtgg ctgccttcgg gtatcgacta tgcgacgtgt catgcggcgg
    21781 cggcgatgat cggcgcgatc accaccgggc tgaacccgag acttggtccg cgggagatcg
    21841 aatcgattct gtctcaggct gatccgtcat tggtcgtggc cgacgagcgg ctcggcgcga
    21901 tcccggccgg cggacgtcgc gtgttgccgc gtgcatcgat gtccaccgcc cccgtccacg
    21961 gggatctgcc tgcggtggaa ttgaacccgc acgatcccgt gagtctgatc ttcagcagcg
    22021 gaaccaccgg gctgcccaag ggcgcggtgt acgacgccgc aggcctggca gccggcgcga
    22081 tgtcggcagg tgtgatgagc gcaccatttg accgcagact cacgtctaca ccgttcgccc
    22141 acgccggcta catgttcaag ctgtgggatc aactgacgtg gggcaccacg ttggtggtcc
    22201 cgccgacacc gtggtcggcc gcgggcatgt tcgacgtctt gcgcacggag agaatcaccg
    22261 ttgccggtgc ggtacccacc caatgggcca agctgctcga aatcgaggat gtaggtcggg
    22321 agtccctgcc gcatctgcga attggagtcg tcgcgaccgc gccggcttcg cccgaactgg
    22381 ttcggcgcgt ccggcaacgg ttcggcgtcc cactggtcgt gcgttatgcg atgaccgaat
    22441 gcccgacgat ctgcggaacg gcgcccgatg acccgccaga gatccaatcc gcgacggtag
    22501 gccgtcccgc ggcaggcatg gaggtacgcg ttgacgacgg cggcgtcgtc gaggtgcggg
    22561 gaccgtgtgt gatgcgcggc tactggcgta atcccgtgct gaccgcggag gtgctggggg
    22621 agggctggct gcggaccggt gacatcggaa cgctcgacgg gaacggctac ctgtcgatcg
    22681 tcggccggac tggcgatatg tatatccgcg gtggctacaa cgtgcacccg gtcgaggtcg
    22741 aacgagtgct tgccgcccat cccgacgtaa aagaggcagt cgtggtaggt cggtccgctc
    22801 cggtgatcgg cgagatcggt gtcgcgttcg tcgtgcccat tgatccgagg aatccgccca
    22861 cgctgcaaga actccgggct tgggtgtccc gccagttggc ggactacaag gcaccggacg
    22921 acgtggtagt cgtcgatgcg ctgcccttga cgccgatgct caagccggac cgggcggcac
    22981 tgcgcgcggc gaccccacgg tcctcatgaa agaagatttg cgctggcaat tcaacgcgcg
    23041 acgctcgatg ggctcgatga atgtcttctc gaagacgaca aagcacgcta gtgtaaatag
    23101 atatatgtat gtcggataag gagcgtttga tgggaaatct ggatggacgc gtcgcattga
    23161 tcacgggcgc cgcgcgcggg cagggccggg cccacgcggt ccgcctggcc atagagggcg
    23221 cggacatcat cgcggtggac atctgccgcg acatcgacac catggactac ccgaacgcca
    23281 ccgcggacga tctcgaggaa acggtgaaac tcgtcgagaa cgaggggcgc cgaatcatcg
    23341 cgcatcaggc cgacgttcgc gacgccgacg ctctcgatca gatcgtggga gacggggtca
    23401 aggacctcgg ccgcctcgac atcgtcatcg ccaatgccgg catcatccgg ctgggcggcg
    23461 gtggcgaccg ccgtcagatc ttccgagata tcgtcgatgt caacctgttc ggcgtgtgga
    23521 acaccgtgga ggcggccatc ccgaaactca tcgagggcgg ccgcggcggc tccatcgtgc
    23581 tcaccagttc gagcgcaggc ctgaagggcc cgaataccga ccgcgcaggc gcgcaggcct
    23641 acaccgcctc caaacgcggc cttgtgggtc tgatgcaggt gtgggcaaac cacctggcgc
    23701 agcattcgat ccgcgtcaac acgattcacc cgacgggggt ggccaccggc atggtgatga
    23761 acgagacgat ggcggccctg gtcgcggccg aggacgtcgc gataacggcg atgcagaacg
    23821 cacttccgat cccggtgctg atgcccgaag acatcgcggg cgccgtcgcc ttcctggtat
    23881 ccgacgaggc gaagttcatc accggcaccg catggccgct cgacgccggc tttgccgtcc
    23941 gttcgtagcc aggatgtcag ggctgatccg ggcgcagctc gatgaacagc ccctcggctt
    24001 cggcgcacag gacgtcgccg tgagtgagcc tcgcccggag aatccgtttg cggccctcct
    24061 cgctgacgaa ccaggcgctc acctgaagct cttcgtcgac cggcgtgatg gaacggaagt
    24121 cggtgtgcag ataggccgtt cgtgcggggg cccgatcacc gctactggcc agcctgccaa
    24181 gcacctcgtc gaaaagcagt gggatggcgc cgccgtgaac ggcgccaccc ccgccgagga
    24241 agtagcgccc gaacgtgacc ctcccctcaa ccttcgtgtg gtcgcccgtg atcgggacga
    24301 agttaggaga catggtctgc ccgcggccgg gcaggtcgag ccgccgagcg aagatctgcc
    24361 tgcgctccgg gactgcggct cctgcgagcc gatcggtcca cgcgcgcagg tcctccgtca
    24421 gcgccaccgt cgtctcggtg gtcggggcag cggcggctac gccgtcgagg aagtcccgca
    24481 gtgcggtgat catgtcgccg tactcagcgc cgccgccggg actgcgaacg ctgggttcct
    24541 gccacgtccg catcggtggg aatccgcccc gggggtcctg gtcgtcggtc acgttgcgca
    24601 ccactcggtc atatccggag actaatgcat tgacattaac ttcggtcgct gagatgctgg
    24661 cgtggcgtgc ctcacagcac gtctgttgag atccgcgagc acctgtctag ggagcgaaaa
    24721 tgccggaagt cgatttggcg ccgcacggtc cgctggcagg gctcgttgtg gtcgacctgt
    24781 cgacgacgtt gccgggtgcg caggcaacgc agttcctcgc cgattgcggc gccgacgtca
    24841 tcatggtcga gccgcccgat ggcagtccgc ttcggcacct cgcgggatgg cccgcgctac
    24901 tgcgcggtaa gcgcagcatc accctcgacc tgcacgacga ttccgacctc taccggttgc
    24961 gtgcgctgct gcgccgagcc gatgccatgg tcaacacgct gcggccggct gcggccgagc
    25021 gcctcggctt gacccccgag ctattggccg agcggtatcc gcgactcgtg gtcggcgcca
    25081 tcaccggctg gggctcgacc ggtccgtggc gcaactacaa agggtgggaa gcgctggtca
    25141 tggcgaagac cggcgtcatg cacgagaagc gggggctgac agcacgtccc gggccggcct
    25201 acatcgccgc agcgtatgcg tcctggggcg cggctcacgc tgcggtgcag ggcattctgg
    25261 ccgcactgct cgaacgggaa tccagcggcc gcggtcagat cgtggagacg aatctcgtca
    25321 ccggcatggg ctcgatggat ccgtacaact ggttctacga gatggtgctg gagcgttatc
    25381 ccggtgcctt cgagccgatg gacgcggcct acgacgacca ggggcgcccc caggcgtacc
    25441 tcatctatgc actgctggtg gcgccgacaa aggacggccg gtggctgcag ttcgcccagg
    25501 tgtctccccg gctgatcggc gcgtggctga ctgaactcga cctgatgggt gagatcgccg
    25561 acccgaaatg gcaaggcttt ccgatgctgc cgacgcccga gctgcgcacc gaatggtggg
    25621 atctgatgct cgagcgggtg ggggcccgca ctctcgcgga atggcagcag gcgatggagg
    25681 tcaacaagga tctcagcggt gagctgttgc gcagtcccga cgactcgttg aaccatccgc
    25741 agaccgctca cgagggccgc gcggtcaccg tggtggaccc cgacctcggt ccggtgcggc
    25801 agccgtcgac cctgatccac gccgacggca cgccgctgac cacattgcgt cccgctccgc
    25861 gcctcggcga acacaacgac tcggtgagct ttgacgaggc gacgggtgca gcgccgccgc
    25921 cgcaggaccc ggcttccgac ccgccgctgg cgggcgtgac agtcctggaa ttcggcagca
    25981 tgttcgccgg gccctacggt gccactctgc tggccgacct tggtgcgcgc gttatcaagg
    26041 tcgagccgct cgagggtgac aacatccgca cgcttgtcgc ctttcccgaa gccggcggcg
    26101 gaaaggtgtt gcagggcaag gagagtgttg ccatcgactt caccaagccc gagggactcg
    26161 agctggtcta cgagctcgcc aagctctccg atgtggtact gcagtgcttc cgaggcaagg
    26221 cggccgagcg ggccaagatc gacgaggcat cgctgcgggc cgtcaatccc gacctggcat
    26281 ttgtgaccac gtccggctac ggcgtcgacg gtccgttttc gcaccgtgcc gcctacgcgc
    26341 cctcgatcgg cgcggcctcc ggcctgtcgt tgatcgacag ccaagacgac ggccacgcgc
    26401 ccgtcgacct ggacgacacg caccgcaggg cggtgaagtt gcacgcgggt ggtgcggtgc
    26461 cggccgtgca atccgacggg atcgccgcac acggcgtcgg ctcggcgctg atggtcgcgc
    26521 tgtacgcgaa gcgtcgcggc atccagctcc acaatctggt caccacgatg cttggcaccg
    26581 tgcagcaggc actggtcgta cacaacacgg gatacgccgg acggccgacg atgcaagccg
    26641 ccgacgacca gcacttcggg gtcactgcgc tctaccggat gtaccgcgcg gccgacggct
    26701 tcgtcttcct cgccgctccg ctgccccacg agtggccggc actggcaaag gccatggcgc
    26761 cctacgtcga gctgcatgtc gacgaacgat tcgcgagtgc cgaatcgcgc gcggagcacg
    26821 acgaagccct catcgaggcg ttgacgccgg tattcgcgac gaagagcaaa gtggagtggg
    26881 aagacgagct gacggctcag gatgtcggct gtgtcgaggt ggcggaaagc aactccgagc
    26941 tggtgctgca gaccgacccc tacttcgagg cgggctatgc ggtgcaggct tacagcccta
    27001 ttttcgagga gcatcgccga ctggctccgt tgtgccggtt ctcccgatcg cgttccaggg
    27061 ccgaagcggg ctgcacgatc ggccagcaca ccgatgcggt gctgcgcgag atcggactcg
    27121 gcgacgcccg catcgccgac ctgcgctcac gcgaaatcgt cggcggcagc tagcagtcag
    27181 gcgtcggccg atccactcat cagagcgcgg gtctcatacg acaccggcgc ggtgagcatg
    27241 ccgacccagg tgtcgacgta gttgtgcaat tgcgcctcgc tgagcccgtg gcccgagcgg
    27301 gcggccgcag ccagcacgtt gatcaggctg acgtaaagct ggaacgttcg gccgcgccgg
    27361 atccgctcgg gcaggtcctg aagcgctgcg tcgacgagtt cctccaaccg ctcctgggtc
    27421 cactcgtcct cgacgtgatc cggccagtag tcgctgcggg cccgcggatc ctccacgagc
    27481 cgcgccagaa acggtgcgaa catctcgccc gccgcgatgc tgttggcgag cgggcgggcc
    27541 agcagccata caaccgcgcg gggatcggct tccttaccct gctcacgcat gctcgcgagc
    27601 atctcctgtc ggtctgctcc gagcgatgcc tggcgatagg agatcagcgc tcggatcagg
    27661 ccgtcgcggg agccgaagtg gtaccggatc accgacgtgt tcgactgccc ggccgcctgc
    27721 tggatctccc gcagcgtcac cgccgcgatg cccttggtgg cgaacagccg ctcggccacc
    27781 tccatcagca tcacgcgggt cgattcgccg gacgagttgc gacggcgcgt cacggccgtc
    27841 agctctgctc cgacgttcgc catgcacaca ataataatgc agatctatta gaaaatctgg
    27901 acttcggccc gtcggcctgc catcatcgaa tgaagcacag cagccgttgg aggacctaat
    27961 gagcctgatc tggacgaact cgggcgattc gcatttcctg gagcccgacg acctgtggca
    28021 gtcccggttg ccgaaggcgc tcgccgacct gacgccgcgc gcggagaagg atcccgacgg
    28081 cgagtacgag accgtgtcgg tggacggaca gatcttccgg cgcaagctgc cgtcgtcggc
    28141 gatgacagcc ttcatcgagg agagcatgaa gccgcagggc atccgcgacg ccgaggcccg
    28201 gatcgccgac cttgacaagg aaggtgtctg gggcgaggtg atcttcccgt cgctgggcat
    28261 gtggggatcg accttccgca ctccggaact gctcaaggcg tgcatgcggg ccagcaacga
    28321 gtgggcgctc gaggagatct gcgcggtgtc gccgcggtac gtggtaaccg cgcaggtgtc
    28381 gaccctggta gtcgaggatg cagtcgagga gttgcactgg gcggcagaac aaggcttcaa
    28441 ggcagtcttc ctgccaacca caccgcatcc cagcgctccg gactggcacc gcaaggaatg
    28501 ggagcccttc tgggctgccg ccgaggaagc cggcatggtg atcgccttcc atatcggcac
    28561 cgacccggtc gacatgaccg tgggcggtgc atctggcggg gcggggcagg tctaccgcgg
    28621 accgggtggc gcaatcatga actacacaga gacgacgttc tcgggtcagc gcgcggcgat
    28681 gaagatggtc gccggcggcg cgctggaccg gcaccccaac ctcaaggtct tgatctccga
    28741 ggggggcgcg acgtgggtgc ccttccttgg tgaccgactg ctggagggct accggcagca
    28801 ccacatggcg gtgcgcccca agctttctcg caaccccaag gagatcctgt tcagccaggt
    28861 ttatgcctcg ttccagcacg acgagacggc ggtcgcggcc tacgaccaca tgggctacaa
    28921 gaacgtgatg ttcggcagcg actacccaca catggagggc acgttcggcc acacacagga
    28981 cacgttgaag gggctgttcg acggtgtgag cgacgagacc cggctgcgga tcacccaggg
    29041 cacgttctac gaactcttcc cagacgtgcc acctatcccc gccgaaaagt tctgagcgcg
    29101 tggcaaatac atcggggttg cgcgacgccg ccatcgtcgg tgtcggcgcg acgccctact
    29161 acaagcgcgg tacgtcgctg ccgaaaacca ccaccgagct cggctgtgag gcgatcttgg
    29221 cggcttgcga ggacgccggg gtggccgtga ccgacatcga cgggttcgcc tactactcgg
    29281 gcgccagcgc cgggtacacc gaaaaaatgg acacggcaga cttcatggaa accctcggga
    29341 ttcccgaagt ccggttcacc gccgcgctga cctcaggcgg cggtggttcg gcgggcgcga
    29401 tcggactggc gcgcgcggcg atcgtcgcgg gtgacgctaa cgtcgtcgtc acgttgatgg
    29461 cgcttcaaca gtccaatcag cgtcttggga ccgtgttttc ggcagtgccg cccgacccca
    29521 tgacgtcctt cctgcagccc gcagggctgt cggggccggg ccatctgatg tcggtgttgg
    29581 ctcggcggca catgcatctc tacggcactc gacgcgaagc gttcgccgag atcgccatgt
    29641 ccacccgggt caatgcgatg aaccgcccga aggcgatgca ccgggagccg ctgaccctgg
    29701 aggactattt caacgcgcgg atgatcgcag aaccgttgtg cctctacgat ttctgtcagg
    29761 agactgacgg cgcggtcgcg gtcatcacca ccagtgccga gcgagccaag gatctacgcc
    29821 agccgcccgt gccggtggtc gccgccgcgc acggcggtgt acgtgactgg ggccgcgcgt
    29881 tcgcctggat ggggatgccg gacgagtact tcgcgtcgtc gggcaacaag ccgatcgcga
    29941 ctcggctcta ccaacaggcc ggcatcactg ccgacgacat cgacgtggct ctgctctatg
    30001 accacttcac ccccatggtg ctcatgcagc tcgaggacta cgggttctgc gccaaagggg
    30061 agggcggccc gttcgtcgag agcggggcga ttcgctttga cggaggcgcc attccggtca
    30121 acacccacgg cggccaactc tcggaggctt acatcatcgg gatgacgcac atcatggaag
    30181 gggtcgaaca gatgcgtggc accgcgatca atcaggttcc cgacgccaaa ctggcgttgg
    30241 tcaccggtgg acctgcgagt ctgcccgtca gcggactgat cctggggaaa gccgcgtgag
    30301 catcgtcgag gcgaccctgg cgaccacgat ccccggcgag cacgtctgca tcgcggtcaa
    30361 caaggacaca gagccgttct gggcggctgc caaggaccgt cggctggtcg caccgcagtg
    30421 cgccgactgc ggtaccttcc ggctgccccc gactccgttc tgcccgaatt gccaatcgaa
    30481 acaggtggat tgggtcgagc tcagtggtca cgccatcgtc tacagcttcg ccgtcgtcca
    30541 cggctttccc ggtatgcccg acctgcttct ggtgcctgcg gtgctggacc tgcccgacgc
    30601 acccggggcc agattggtgt ccaacatcgt cgatgtcgcg ccgaccgacg tgtcgatcgg
    30661 aatgtctttg cgcgtggact tctcgcccat atcggacggt tggatgctcc cgatctttcg
    30721 cgccgataca gcgatcgcat aaggagtggg tgacgtgtcc gatgacagtg cactgcacga
    30781 aaagccgctg gagttcctca ccggcatcgc caacaccggc gggtcgtgcg acggccaggc
    30841 gatcctgccc gagaacggtc actaccggtg cacctgctcg tgtgacaact gggacatcgt
    30901 cgtggatgac ccgcaggaag gcttgcgcca ggccagaatt cacaccggta gtgtcagctg
    30961 agcgagcgga gaggtcatga ctgatcgagc ggaggtcgag cgcgagttcc ggcgttattt
    31021 catgaccggc ccggtgctgg aggactggca cgcctgggcg agcctgttca ccgatgaggc
    31081 gacatatttc gaccacttct acggcacctt caccggaccc gaagagatca ccgggttcct
    31141 ggaggggacg atgggcgccg caccgcaggt gtacagcccg ctactctgga acgtcatcga
    31201 cggcgagcgc gtcgcctaca aggtcttcaa cagggccgac aacccagaac ccggaggcag
    31261 tcccatcgaa ttttcgtcgt atcagttcat cgaatacgcc ggcggtggga aatggcgctc
    31321 ggaagaagat gtgtgggtat tggccgagat gaagcgcttc gcttcgcaat acgccgaggc
    31381 cgaggccgcc aacccgcaat cgttgcagca gaagctgcgc agggaagact ggggcccgtg
    31441 ggtggagtgg gcaaggccgg aaccggagca ccaggccaac ccgtcctggt tgggcaagga
    31501 gggattcacc cccttctcca gcatccgtga catcgacttc ggcgttcggt cacactgacc
    31561 gtgcaaggcg cgccggtctc tttaacgttc gacgtcacgg caggtatggg aacgggtgag
    31621 cggttgactc aggcagcatg ggcgtttcta ccggaccggc ccgccgatgc gtctgccgtg
    31681 cttctgtgtc tcgccggcgg cacctacgac aagcactact ggcacatcga gattgagggc
    31741 catcccggct acagcttcgg tcagcacctg gccgatgccg gcttcatcgt catcgccgtc
    31801 gaccacctcg gcatcggtgc gagcaccgac cctgttgcat ccggttcgct cggcttgcac
    31861 ttgcttgccg caggcgacgc cgaagtggcg cgacaggtcc gagaaggcct gaggcatggc
    31921 acattgctcg acggggtccc gccgaccgcc acgccgttgg tcggagtcgg acattcgatg
    31981 ggtgcctgcc tgaccacgat ggtgcaggcc actacccacc cttacgacgc agtcgtgctt
    32041 ctcggctacg gagttcagat caccaacgtg tacgaagaca ccgccgacgc agccgaactc
    32101 gaacagcgga tccagcagac gatcgaggcg gcgtgccaac tcaccggcgc caaccccggc
    32161 gacagccaca ccatcgcgcc acgcgactac ctcgccgaac tgttctacgc cggggaggta
    32221 ccgcaggcgg tcgtcgatgc cgacacctcg gcacaaagcc gtgtgcccgt gcgggcggcc
    32281 gcagaggtca ccacgcccgg tttcgtcgag cactacacag caagcgtcga cgtacccgta
    32341 ttgctggcat tcggcgccgc cacggacgtc tcgccgaatc cccatgcgga gccggccaac
    32401 tacacatcct ctgccgacgt caccctgcac ctggtgccca aatccggcca ctgccataac
    32461 ttctcgagtc accgcgcaca gctgtgggac cgcattgggg tgtgggtgcg aaccgtcacg
    32521 tccgggagta ttgaatgacg aaggtcatcg gattcatcgg agcggggcaa ctcggtgagc
    32581 cgatggtgtc gcggctgctt gccgccggtc atgacgtgct ggtgtacgcc cgccgggccg
    32641 aggtgcgcga ccgggtggcc tcgcagggcg ccgccctggc cgattcggtg gcggatctgg
    32701 ccaggcgcag cgacatcctg atctcgtgcc tgttctccga cgcccagttt cgcgagatcg
    32761 gactgggtcc cgacggtgtg gtcgcaaacg cccgtccggg tgcggttctc gtctcacaca
    32821 ccaccggcac cgtgtccacc ctgaccacgc tgcgggacag tcacccgtcc ccaccgctca
    32881 tcctcgatgc tccggtcagc ggcaccgtcg agagcatcaa cgcgggcacc ctgaccgtgc
    32941 tgctcggcgg acccgctgat gcggtgcaga tggtgacgcc ggtgctggcc gcgtatgccg
    33001 atcctatcgt gccgaccggc gaactgggca gcgcactggc catcaagctc gtcaacaact
    33061 tgctgttttc ctgcaacgct caactgctgg ctgcggcaat gcaattgggc caagcgctcg
    33121 gcgtcggcga cgacgcatta ctctccgcgc tgcaggtttg cagtagcgcc agccacgccg
    33181 cggcccaggc ccatcgcgtc ggcggaatcc agcagttcac cgagagggcc acgccttttc
    33241 tgcgtaagga catcgccgcc gctgcggagg cagccggcga ggcgggtgta gacctcggat
    33301 tgctgggcgt cgtcgcggac tcaggtccgc tggagttgtg aggccgcagt tcgggtccta
    33361 ggcctgctgc aagtagtgct cgaccagccc aacggaggct tgcggatagc ttcgcgcggt
    33421 tgcctgatcc aggccgtcgc gcaccagcag gaccgcgagc ctgttgttga tcagtgacag
    33481 cacgaacgct gcaagcagtt ggtagtagcc gaggtcgccg gaaagtcgtc cgccgaattc
    33541 ccggtatcgg cgcacgaatt cgtcgtcgtc tggaacgccc ggtggccggg tgaagcccag
    33601 ggactcgcac aggaacgtct cgaacatcag ccaccaaccc agatcgatat cgggtggccc
    33661 ggtggtggcg gtctcccagt cgaacagtgc gaccaccgac aaatcgtcgg cgaagcacat
    33721 gttgccgacg cgtgcatcac cccacaccac ggtctctgca tcggttctcg gggccgagtc
    33781 cagcagcacc gtccacgctc gggcgagtgt ttgcgcaccg accttcacgt cgtcgccgca
    33841 ccattcatac cagtaggaca acgtctgcag gtagcgctgc agcgcggtgg gaccctcggg
    33901 ggagcccgtg cggaggaact cgaagtcagc ttcgtcggtc accctatgca ccgcgacgag
    33961 ctcgcgtaac gcgttgtcgc agagaagctc gcgatcggac acagacaatt cggcggtcca
    34021 tccgcgcttg tgccaactgg gcacatccga cggtgttcga ccatagaccc tgcgcatcag
    34081 ataaaacggc gatcccaaaa ccgaagcgtc ccgctcgatt ccgacgattc tcgggacagg
    34141 cacaccatgt gcgcccagcc gctgcatcac ctcggcctgc ctcggcgcgt cgggggtggt
    34201 gaacaactga tggctgtcgg tctgcatccg caagacgagg tcctccgttc gatgacgccc
    34261 tgtcagctcc gtccattcgg cggtgacgaa gaccgtctta ccggagtagc ccgcgtcggg
    34321 gctgctgaac gacgagatgt cgaagctttc ggcacctgca ggcgcaagcc ttgcgggaag
    34381 ccaggaacga aggacctcgc ggacccctcc gcccagctca cccctggccg gtgtgtacgc
    34441 caccgaaggc cctcctagcc atgagtgaac actactgtca gaataggtga gtggcgggcg
    34501 tcgaatccag cagggtggca ctcggtgcct acaccctgcc ggggcgtgtc tccaatcccc
    34561 gggcagcgat atcgcaggcg gcggcagcag agcaactcgg gctgcggacg ctgtggctca
    34621 gcgaacggtg gggtacgaag gacctcggcg tcctcgcggg cgcgctcagc caggtgacga
    34681 cgaccatccg gatcgcttcc ggcatcacac actttcagtc ccggcatccc gctgtccttg
    34741 cgtcgctggc catgaccgca caggccatgt cggatggccg catgatgatc ggcgtgggcc
    34801 gttcggtcga tgcgatgtgg accgcggtgg gcctgccgcc atcgaccaac gcatcgctga
    34861 tcgaccacgc cgacatcttc cggcggctgt gccggggtga gaaggtgcgc tacgacgggc
    34921 ctgcgggcac gtttccgtcg ttgcggctca acgacgttcc cgatcaacca gtgccgccat
    34981 tggtgttggc cgcgatcggg cccaggggcc tggcgatggc gggccggcac tttgacggcg
    35041 tgctgctgca cccattcctc accccggacg ctgtccgacg ttcggttcgt gtcgtccgcg
    35101 acgcggagtg tgccgcgggg cgccccggcg gaagtgtcgg ggtgtacgcc accgtcgtcg
    35161 tcgcgtgcga tctacctccc ggcgagcagt cctcggtggt cggcgcccgt gcggtgactt
    35221 actaccagct gccggggttg ggggagcggc tggcggaggt caacaactgg gatcccgatg
    35281 cgttgacgcg gttgcgttcc catccgatcc tggccggcgt gaagggttcc gcggactcgg
    35341 tcctcacccg ggaccaactc gtcgacgtcg tttcgagcct gcccgacagc tggatcgccg
    35401 acgcggccgc ggtcggatcg gctcgtgaat gtttcgacgt gttcgaacgc tatcgggacg
    35461 ccggggcgga cgaactcgtg ctgcacggta tgacacccga tcggatggcc agtctgcttt
    35521 cgtgacgggg gcgacgggct aactgcccag tcgcaacccg gtattgcgag tgccgaagat
    35581 gtcggtggcc atgatcgtcc cgagcttgtc cgcgtcccag cgtttctggt tgttcgacac
    35641 cgtcgtaacc ggttgccagc ccttgagcag ttgtaggttc tcgccgagcg ctcggatcac
    35701 ctgcccgctg acgtgaccgg cctcggggct ggcaagccag gccaccaccg gtgaacccag
    35761 cgatgggtct ttcgggtcga actcgtcctc agcacgctcg tcgggttcga tcggcgcggc
    35821 ggcgccgggc atcgtggcgg acatcctggt ccgtccgccc gggctgatga cgttcgcggt
    35881 ggcgccgatc gatgccagtt ccaggctcag cgtttgggtc agcccgacga tcgccgcttt
    35941 cgccgcgctg tagttggtct gcccgaaatg cccgtgcagg cccgcgccgg atgtggtgtt
    36001 gatgatccgg ccgtacacct tcgagcccgt ctccttggac tcgacccgcc acttgcgcgc
    36061 gacagcgcgg ctggtaagcc agctgccccg tacgtgcacc cgcatcacaa ggtcgaagtc
    36121 gtcgacgctc atgttccaga tcgccttgtc acggacgatg cctgcgttgt tgacgacgat
    36181 gtcgagcctg cctagttggg agtacgcccg ttcgacggca agatccacct gctcctcgtc
    36241 gccgacatcg ctgaagtccg agaccgcctt gccgccacgg tcttcgatga tcgcgaccac
    36301 ctcgtcggca accttgcctg tgccctcacc ggcgagtgtc gtgcccaggt cgttgacgat
    36361 cacggtggcc ccgtgtttgg ccagttctag tgcgtgaccg cgaccgatgc cgtgcccggc
    36421 gccggtgaca agcgcgatct tgccgtcgag tagacctgtc acgttggctc ctcgctaggg
    36481 gtgaagacgg tcagatacgg gctctcgggg tcgtcggcgt cgacgggccg gaaggaagcg
    36541 gtgactgcca ttcccacccg caacgcctca ggttgggcgc cttcgatggt tgtcatcacc
    36601 cgtgggcctt cggccagttc aacgatcgcg gccacgtagg gcagccgctc gcgaaacggt
    36661 ggtaggtcgt tggcgtacac cgtcgagtag gtgtacagcg tgccggttcc actggcccgc
    36721 aaaggatgta cgtcatcgct ccagcagtgc gggcagaagg gccgaggata atggtgcacc
    36781 gtgccgcagg ccccgcactc ggcgatcagc aaccggccct gcctggcgcc gtcccagtag
    36841 ggtgcgctgg ccgcatcgat gatcggaatg tctgctcgcg ccacgactac ccgccggtga
    36901 actcggtagg gcgcttgtcg atgaacgcct gcacgccttc tttggagtcg tgggagtacg
    36961 actgaacctc ctgtgccatc gcctcagcca cgaacgcgcc tgcgcggtcg gtgtcgggcg
    37021 agtcgttgag caggcgtttg gtgaaggcca ccgcagtggt cggcgcggca gcgagacgat
    37081 cggcgaagtc cgccacggcg gtgtcgagtt cgtcatccgg aacaacccgg ttcaccaacc
    37141 ccagtgtcaa agcttctgag gcggtaagtc gttcaccgaa aaatgccatc tccttggcct
    37201 tctgcatgcc gatgcggcgc ggtaacagat agcagccacc gccatcgact accaggccac
    37261 gtttgacgaa agattctgca aagtaagcat tctcggtggc caccaccagg tccgacgcat
    37321 agacgatgtg tgcgccgacg cctgttgcgg cgccctgcac tgcggcgatc accggcttgt
    37381 tgcaatccag cacgctggcc acgagcttct gtgcaccgtt catgattcgg cgcatcggat
    37441 ccatgacccg cttctcgacc gtggcgcgcc ggtcggccaa cgacccgacg tccgcgcctg
    37501 cacagaaatg cctgccgttg gcgcgcagca caacgactcg caccttctca tcgccgtcgg
    37561 cggcgcccag cagcgcgatc agcgcgtcgc ggtcctccgg ccgtaccgcg ttggcggcct
    37621 ggggccggtt cagcgtgatg gtcatgacgc cgcccgccat gtcggacagt atcgtctgct
    37681 cgttgctcac ggtgtgtagc ttgccgtcaa gtgtttgatg ccgttgacga aattcgaacg
    37741 cagcatcacc ggctcgccaa ccgaggcgat gtcggggtag cggtcgaaga gcatcttgaa
    37801 cgctacgttg agctcgagcc gggccagatg tgcgccgagg cagaagtgcg ggccgggacc
    37861 gccgaacgca acatggttgt tgggggtgcg cgcgatgttg aacacctctg catcggcgaa
    37921 cagcgccggg tcgcgattgg cggccggata ccagacgacg accttgtctc ccgcgttgaa
    37981 ggtgtgggtt ccggactcga gcgccacccc gtcgcgagtg atggtgcgcc gcatgtggat
    38041 taccgggctc gaatagcgca ggatctcttc gacggcggtc gacgccaggg tctcgtagtc
    38101 ggcgacgagc cggttacgct gctcgggatg accgctgagg atgtgcaggc cgtgtgtcag
    38161 cgcattgcgg gtggtctcat tgcccgcacc gatcagcagg atgaagaact tcgccagttc
    38221 ctgcggcgtc aggttctcct cgtcggacgt gacgagcttg ctgatcacgt cgttggtcgg
    38281 gcgggcgatg cggtcctcgg cgattccctt caggagctcg atcagctctt cgctcgtctc
    38341 ggtgatggcc gcctcgatac ccgcagcggt ctggtcggga acatattcgg gatcggatgc
    38401 acccagcacc acgttcgtgg ccctcaagat gaactgttcg tgctcgcggg gcacgcccag
    38461 catgttgtcg atgatgcgca gcggcagcag cgtcgcgaaa tcggcgacga aatcgcaccg
    38521 gcccgatgcc ggcatctcgt cgaggatctc ggcggtggtc tcctcaacca gcccacgtag
    38581 ttccgacagc atccgcgggg tgaagccacg cgagacgatc ttgcgcagcc gcatgtgttc
    38641 cggatcgtcc atgtcgatga tggagccgcg gtactcgcgc atctccgggg tctgatcgaa
    38701 aatctgggtg ccctgcccgg agctgaactc ggtgggacgc ctgctgatgt cgaccacgtc
    38761 gacgtgagcg ccgaccgccc agaaaccacg tgccttcggc gcgtcctggc gattggtacg
    38821 gacgaacacc ggacgccccg cctcacggag ccgtcgatac aggtccggcc gttcggcagg
    38881 gcactcctgc caccacccca ggtcgatgag tggggcgaac tcacgttcgt ctgtatgcac
    38941 gagcacccgt caattctggc attagtgtca ctaaagtaca acagcccgta gccagctgaa
    39001 agagccgaat atgaaacgac ggcgctacgc tctgttgtac agttctgact atcctgtcat
    39061 taccgcgaag ggatgtatga gtggatctcg ggctgcgcga caaggcgtac gtcatcatcg
    39121 gcggatcggc ggggatgggt ttggccgccg ggcgggtact cgccgcagac ggagcctcgg
    39181 tggtcgtcgt cggacgtgac caagcccgag ccactgacgc cgccgccgca ctggcggatt
    39241 ccggcgccgc aaccgccttc ggtctggcgc acgacgcgag ccgacccggt gaggcggtgg
    39301 ccgccgtcgg agaagcggtc gggctgctcg gccggctgga cgggatcgcg atcactatgg
    39361 gcaccgccgg catgatgccg atcgactccg atgacgaggc gtgggaggcc gcattccgcg
    39421 atgtgctgct tgcgaccacc cgaagtgtgc aggctgcgct gccgcatctg gccgacacca
    39481 agggctcgat cgtcaccact gcggcgtatt cggcgcgggc tccgcatgag ccgcggctgc
    39541 cgtatgccag cctcaaggcg gccgtgtcga cgttcacgcg cggcatcgca cgagaacacg
    39601 gcaagaacgg catccgggcg aactgcatcg cgccgggagc ggtggaaacc gatgcgctgc
    39661 acgcgattcg cggctacatc gccgagaaca aagggtatcc ctatgaggag gcgctcgagc
    39721 gtgggctggt cgaggacttc gggttcgacg ccgccctcgg acgccccgga caacccgacg
    39781 aagtcggtgc gctgatcgcg tttctgttgt cgcagcggtg cggcttcctc actggacaga
    39841 ccattggcat cgacggagga gcgccctatt gacgtcctcc cctggctgag gccgggggat
    39901 ttcaacccca tctacctaac ctatgcggca ggagagcaga gttgaggttc acggttcgca
    39961 gaccggctag cgccgaggtc tctccgtgct gtcaccgccc gtccggcggc gatgttgcgt
    40021 gcagcattca cgtcggcgtt gcacagccct gcgttgcagg cttcgcactc gaagaccgct
    40081 tggctcttac ggttttcgag cgcgacatgc ccgcacgcgg agcatcgttg cgacgtgtac
    40141 gcggccggga tctgctcgac ccgaccagaa gccttgtgca gcaaacgcgc agccaacaga
    40201 ccccatccgc ttctgctgat gccacggttc agactccgct tttgtgcgac ccgcactccg
    40261 ggctgctcga ccgttctccg cgcgctgcgg gtcatcgcgc gcacgtcaag agcttcgatg
    40321 cggaccgtgt cgaagcgccg cgcgatatcg gtggtcgtct tttcgaccca atccttccga
    40381 cgatcggtct cccgcgcctt gagttttgcg atcgccaact tcgtgcgagc gcgtcggtgc
    40441 gagccacgct cggcgcggcc gaggcgctgc tgcagtaccc tcagccgctt cgactcaccc
    40501 ggggtgagac taggtgcgtg gagcagttca ccggtcgaca acgcggccga caccacaaca
    40561 ccgcggtcga tacccaccac gctgccatcg ctgggaccgg tgatcggacc agggacgtgg
    40621 gcgaaggcga tatgccagcg gccggcccga tcacgggtga cccggtaaga cttcgcccct
    40681 tcagggaccg gccgcgacag ccagaagcgc acccagccga ccttgggcac ccatacccga
    40741 cccaaccggc gattcagacg ctcgacttga tacgacttga ccccgacctg ccgaaaccct
    40801 tcatgcacag cggctttgcg ccacctcggg cggcggtgcg tcccacagaa gaagttccgc
    40861 atcgcctgag cgaaatcgcg caacgcctgc tgctgcatcg tttgcgagcc ggccgccagc
    40921 cagtcatact cggcgcgggc tgccgtcaac tgagcgcact gctcgtgata gccgggcgcg
    40981 tgacgtcccg gccgccagtg cagttgctgt tcgacggcca gggtccacac ataccgagcg
    41041 tggcgacaat gctccgccaa ctccacttgc tgctcagtag tagggagcaa ccgatatcgc
    41101 gccaccctgg caacgtaatc gccaccaccg acaagtcacc gaaaggagag gccgtgtttc
    41161 ctccccctgt taacgccagc gatttccacg ccgcaagtca ccgatgaact tgcgggtcgg
    41221 catcatcgga gtgggctggg gaagccacgt acaggtcccc ggtttccgtg cgacggacgg
    41281 cttcgagccg gtggcgctgt gtgcgcgcac accggagcgg ctggaacgtg tcgcggccaa
    41341 actgggcatc gccgacacct cgaccgactg ggagtccttc gtcacgcgcg acgatctcga
    41401 cgtcatatct gtcgcgacac cgacggtgct gcaccgcgac atgacattgg cggcgctcga
    41461 tgcgggcaag gcggtgctct gtgagaagcc gttggcgggc gatctggatg ccgcacgcga
    41521 catggtgcgc gccgctgaca agtcgagccg gcccaccgcc tgctgcttcg agaaccgttg
    41581 gaacccggac tggttggcgg ttgcggacct ggtcggcaag ggctttctcg gcaagcagta
    41641 cctcgccagg gtaagtcgca gcgcgtcgta ctggcatccg agccatcagc cgcaggcgcg
    41701 ctggatgtac gaccgcgatc aggggggcgg atacctggcc gggatgatgg tgcatgacct
    41761 ggactttctg tgcagcctgc tggggcgacc ggagtcggtg tgcgccgaag tccggaccag
    41821 cgaaccggta cgcgaactgc ccgacggcga aactctgacc gtcaccgccg acgacaccgc
    41881 agcgctgctg atgcggatgg agtccggaat caccgccatc ctcagcgtgt cggtgatggg
    41941 tgtgcacgcc gaccattacc gcctcgaatt gttcggcgcc gacgggacca tcatcggcga
    42001 cggcgatctc cgctctgccg aatacctttc ggggctggcc accgacgacg gcctgagtcc
    42061 gctggcggtc aatgaacgcg aacctgccca ccccgagcat ctgccgaagg gcttggccgg
    42121 acatgcggcg cgggcgatgg cactgatgct gcaggactgg ctgcccgcgt tcgacggtaa
    42181 agacagcgca gctgcgacgt tcgacgacgg actgctcagc cttgcggtga tcgacgccgc
    42241 gcagcgctca gcccaaggcg gcggctggga acgcgtacac gcgaccgtct aactgcgccc
    42301 aaaccgacat ttgggcgcga aagtgcgaga tggttgcgcc atttcgacga tctcggcgtc
    42361 gggctcgaaa gctccgttag ctgcccgact cctcggcggc gggatatatc aatacgccac
    42421 ggatattcgc accggaatgc atgtcggcgt agccgtcgtt gaccttctcc aacggatagg
    42481 tcctggtcac catcgactcc aaatcgactt ggcctgcact ggccagctcg aggatcttgg
    42541 ggatgtccgc gcgcggattt cccgacccgt acaacgtccc cacgatctgc ttctcggtca
    42601 aggtgaggtc catcaggttc gctctgatgt cgcgttccag catcgggtga atgttggtga
    42661 ccacgactcg tccgcgcttg gcggtcatgg ccagcgcctg cccgatcagc tggccgtcgc
    42721 caacacccat cgcgcagatg aaccggtcgg caccccgatc ccaggtcgcc tccgcgacaa
    42781 cgcctttggc ctctgtccag tccgcagcgg tgtgggtggc gcccatcttg cgggcctctt
    42841 cccgcttgta ctccgacgga tcgatcacgg tgatggtgcg cgcaccggcc agtcgcgcac
    42901 cctgcacggc tgccgcgcct ataccgccga ccccggcgac caccaccgtg tcgcccggtc
    42961 gcacgtcggc ggcgtaaacg gccgaccccc atccggtgat gaacccacac cccagcaggc
    43021 aggccaggtc gaggcgatag tgctcgggga tcttgacgca gctcgcttcg tgcacgacgg
    43081 tgtggtgcgc gaaagaccct acgccgcagg ctaatgcgag atcctgaccg gcagcgtggt
    43141 ggcgggccgt ctcgtcgtag atctgcatgc ctgagtagat cttggcgccg aggtcgcaca
    43201 ggctttgatg acccgtggaa cacgatcggc aacggccgca tgacgggatg aagctgaaca
    43261 ccacgtggtc acctggggca agccaggaaa cgtggtcgcc caccgctttc acgacgcctg
    43321 cgccttcgtg gccgccgacg cacggaagcc tgatgggcat gtcgccggtg accaggtggt
    43381 cgtcggagtg acacataccg gaggcatgca actcgaccag gacctcttgt gccttgggcg
    43441 gatccagatc gatggtctcg accgaccacg ggctgttgcg ctcccagagc accgccgcct
    43501 gcgtttgcat caactgtcct ttctgggtgg gcgcacgatg gcctgatact cgacgaaccc
    43561 ggcgattcct tccgggccgc cttcgcggcc gatgcccgac atcttgaaac cgccgaacgg
    43621 gctgccgaag tcgagaactg cggtggaatt gatcgccacc gaaccgactt gcagtcgcga
    43681 cgccacgtcg gcggcccggt cgtcgtcggc cgaccacacc gcgccgacca gtccgtagcg
    43741 cgagtcattg gcgatggcga cggccgccgc gtcgtccacc gaatcgtcgt atgagatcac
    43801 caccgcgacg gggccgaaga cctcttcgcg ggcgatctcc atgtcgttcg tcgccgagac
    43861 gactgcggga gaaacgaacc atcccggccc atcgacgcgg tccccacccg tgagcacggt
    43921 ggctccctgg tcccgcgccc gctgcaggaa cccgtcgatc ctgtcaaggg ccgtttcgtt
    43981 gatgacgggt ccgatctggg tggacctgtc ggccggatcg ccgacgacca ggtcggcgac
    44041 cgccgcggtc agtgcggtca cgaactcatc atatcgactg ctaggcaaca ggattcgggt
    44101 tagtgccgca cagagctggc cgttgttccc catcaccccg gtgacgagtc cggccaccgt
    44161 cgattccagt ggcgcgtcgg gcagcacgat cgctgcggac ttcccgccga gttcggtgct
    44221 gcatcggcgg attcgcgatc cgcaggacgc ggcgatctgt tcgcccaccg cggtggaccc
    44281 ggtgaaggtt atcttcgcga cgtcggggtg cacagtaagc gcctcgccgg tggctgcccc
    44341 gccgttgacg acgttcacca cgccgtcggg cacccccgct tcctcgatcg cctcggtcag
    44401 caccgcgatg ctcagcggcg catccggtgc gggcttgagc acgacggtgc acccggcggc
    44461 aagcgcaggc ccgagcttca acgcggcgat gaaaagtggc gtgttccacg ggatgatcgc
    44521 gccgaccaca ccgaccggca gctgccggac gcgcacggtg cctcccccaa acgcggggcg
    44581 ggtcgattcg aatacataat cgggcgtgat cgccgcatag gcccgaagga ctccgacggc
    44641 cgtcagcacc tgcccgaacg tgctccagga tcgcggcgag ccgatctcgg cagtggtcag
    44701 gtccgcgagc aagcccttgc gcgcgaacag cgcgtcggcg agccgtccga tggctgccgc
    44761 ccggtcggtg acagacgcat cgcgccacgg tggcgaggcc agcgccgcgc gagcggccgc
    44821 gacagcggca tccacctcgg cctgaccggc gttgcccacc gtgcccagca actcaccgct
    44881 ggccggcgac cacacctgca gccggccttc tgcgccggtt tcctgcgtcc atttcccggc
    44941 gacgtagctc gccgtgaact ccaacataac ggctgattct ggcaggaatg tcatctttgg
    45001 acaagcggct atacgtgcaa gaaacgcaaa tatggttccg cacgagccgt ttttctgaca
    45061 gtattggtca ctatgagttc atcgcctgac gcaggtgacg gcccggtagc ggggtcgcgg
    45121 tgggaggtca tggtcgagcg cgggaaaatc gccgagttcg ccgacgccat gggatcggac
    45181 aatcccgcct atcgcgggcc cgacgcgatg atcccgccga cctttctcac caccgctgcc
    45241 cgctgggcgc ccccgggttc tcgggtggac gtcgggttcg accgcaagcg gttgttgcac
    45301 ggagagcagg agtacgtctt tcacacgcca ccgccgagat cgggtgacgt gctgaccgcc
    45361 cacgagcgga tcgtcgaccg cttctccaag ccgggcaagc gtggcggaac gatgcggttc
    45421 gcgacggtgc tcaccgagta ccgctcttcc gacgggacgt tggtcgccga ggccaaggcc
    45481 accttcatcg agaccgcggc ccggcgatga gccccggcga agagcatggg acccagaccg
    45541 cgcccgccct ggtgcgaccc ggcgtcgagg cgccgccccg tgagttcggc ccgttgaccc
    45601 gccaaatgtt cgtccgctat gcgggcgcat cgggagatct caaccccatg cactacgacg
    45661 acgagctggc gcgctccgcc ggctacccgt cggtgttcgg ccaggggatg ctgaccgcct
    45721 cgctgctggc cacgttcgcc agcgattggc tcggcgccga cacagttcgc cgcttcgccg
    45781 tccgtttccg cgagcaggtc tggcccgacg acgtgctgac ctgcgccggt accgtcgcct
    45841 cggtcgtgtc cgacggcgat gccgaccggg tgacggtcga attggcggtt cggcggcaga
    45901 ccggtggcgt tgcggtcacc gggacggccg aattcctaat ctcgcgaaat gcggcgccgc
    45961 gcggcgccga ccgcgccttt cggccatagg ctggttgcac catgtccgaa gcctctatcg
    46021 tccgccgatc cagctacggc ccctccagcc cggcggtcgg cgcccgcgga gcaagcaccc
    46081 gcacccggat caccgaggtg tcgctcgaac tgttcggccg gctcggctat ttcgacacct
    46141 ccgtcgatgc catcgcgaag gcggccggcg tctcgcgcgc cacgctctat cagtacttcc
    46201 acggcaagga cgagatcttc ctcgaactgc tgaacgaatg cggtagcgcc ctgttccggg
    46261 tggcccgccg gatcggcccc ttgggtcccg acgaggtcgg gttcgacaac ctgaattggt
    46321 ggctgggcga gtggagttgg gtattcgaga agtactccac catgttcgtg cagtggaccg
    46381 cgatcgcgac ttcggacacc aaagtgcgcc cgcagatcac gtcgttcgtg cggagttaca
    46441 accaccgcgt cgccgagcgg ctcgccgctt cgggggtgag cggcctcgac cccgaggcgg
    46501 cggcgatgat gatgacggcg ctggtgcacc gcatcaacct cttcgtgcac accgatcgcg
    46561 cctacgggcg tagcgcgaaa tcggcggtgg acacgttgtc ggtgttcctg caactcgcac
    46621 tgttccccga cacgccgccg tcggtgttga cctcgctgcg cttgcatgcc agcgaagacc
    46681 cggctgcaga tgttgatgcg atcgaggtgc cgcctgcgcc cgatatcgaa ggcctatcca
    46741 tcagtgaccg gaccgcgaac ctgagcaagc gggcggtcaa cacggtcacc gcattggccg
    46801 acgcgggtgc aagccaattt cgggcccgcg gctatcgcag caccagcgtt gatgacatcg
    46861 tcgaggcggc cgacgtcgcc cgcggaacct tctacaagta cttcagcgac aaacaggacc
    46921 tgctcgccgc ggtcggcgcc gaaatctatg aagccggaaa ggcgttcgct gaacgcatcg
    46981 ctcgggttga tccgctggac gccgagccca ccctgcgcaa ctgggtggcc acctacgtcg
    47041 agttctacga ccgctattcg ggctgcatcg atgcgtgggc cgaaggcacc gccgacaatc
    47101 ccgtcatcgt cgcgataggg gagaacggcc aggtcctgat ggacatcggc gcggcaagga
    47161 tgttgatcag ccggccgggg ccttacccct tcgatccgat tgtcgccgcg ctgatcgtgc
    47221 gggcgctcgt gacgcgggtg ccgcaggccg cgctcgatct gcccgaaccc ctcgacgacg
    47281 acagcgtcgt cgatctcttg atcagctgta tcaagcgtgg gttcttcggc cgagcgtcgt
    47341 aacgccgcta ctcgtcagct cgtcgatctg ctgcggcgaa taacccaagt cgcacagtat
    47401 gtttgcgctg tgcgcgccaa gtgcgggcac agcgccgagc gacgggtcga ttccggcgat
    47461 cgcggtgggc ggcagatgcg ccatggcgac gccgccgggt gtgcggaact cgcgcagccg
    47521 gttgcgctcg accaacacgg gatggttcca cacctgctcc acgtcattca tgcgcgcgtt
    47581 cgccaccccg gcgtcctcga ggcgctgcgc gaaatccgcg gtagtcagct ccgcgatcgc
    47641 cgcggtgatg atcgactcca gctcgtcacg gtgagccacc cggtcggcat tatcgacaaa
    47701 ccgcggatcg cggaccaact ccgggcggtc cagcacttcc tcacacagtc ggcgccattc
    47761 gggttggttc tgcacggcca gcaatacact tccgccgtcg ccggcagtga acggtccgta
    47821 tggcgcgatg gtggggtgcc gcgctccggt ccgccgcgga gcactgccgc cgtaatggcc
    47881 gtacatgatg ggctgtgcca tccactcgac cagtgcgtcg agcagtgaca cggacaccga
    47941 gcgcacctcg cccgtcgttg ccctgacgta cagcgcaccg agaattcccg aaaaagtgaa
    48001 catgcctgcg gcgatgtcgg cgatggagac cccggctcgc gcgacgtcgg cggctgaccc
    48061 ggtgaccgag agcaggccgg cctcggcctg tacaagcagg tcgtaggcct tgcgatcctg
    48121 ccagggcccg tcgtctccat agccggtgat cgtggcgacg atcagccgtg ggttgcgctc
    48181 cgccatcgcg gcagcgtcga gtcccagtcg cgcggcggca cccggcccca agttctgcac
    48241 gagtacgtcg gctctctcga gtaggcggtg gatgacccgc cggccatcag ggtgcttgag
    48301 gtcaagggtg agtgactctt ttccgcggtt gagccacacg aagtaactgg agtcgccgag
    48361 cacggcgcgg tcgtagccgc gcgcgaagtc gccgccgtcc ggtcgttcga ccttcaccac
    48421 gcgcgcgcca aggtccgcga gttggcgggt ggcgtacggc gccgccacgg cctgctcaag
    48481 gctgacgacc gtgaccccgg ccaacggtgc ttcagcggac aacggatgct cctgacgatg
    48541 atgacggcag tgtcagatgc aacttagcgg acggggtcgg atgtggcgac cgccatcccc
    48601 gtgacccatt cgggaaccgg ctcgtaagct accggcgtga ccggcctgcc accgcctgcg
    48661 gcaccggcgg cgacccaggt cctgatctcg ttcgcacctg cgcccgcccg cgtgtgcgcc
    48721 gaatccacca ggcgtatcag cgtcgcgacg tcccacgccg acatggcttg caatacttcg
    48781 gcatcccagt ccggtcgtat tttgttgcgc gcctctgcca ttccttcgga gatgagccgt
    48841 gcgcgctcgt cgtcgctgag gttgtaggcg tcgacttcca ggctgggtgg tgagtgcgaa
    48901 aggccacccg ttccgatcag cagcacgcgc aagtccagcg tggcgagaaa cccggcgacc
    48961 gctgcgccga attcggcgac tcgccgaccg gtcggtaggg gagcggtcgc gcagttgact
    49021 gggacgggaa tcaccggttt ggcgtccagc gcgccaagca ggtcccgaat aggctgtgcg
    49081 aaagcatgat cgagttcgat gtcacggcaa accgcaacat cgaagtcctc agccaacagg
    49141 tgctcgcaca gcccgcgggc cagatctgac ggaacagcca tgtcgccggc gggatggcca
    49201 ccctccgcca tgatcgacgc cgagagcgcc acgccgaagg taggcaccac atgtcggaag
    49261 gcccgccggt ggtcaccacc aaagacgatc acgacgtcgg gatcgaagcc gtgaacctcg
    49321 tctgcggcct gcgccagtgc agcccggaac ttctgcccga aagcgtgctc gacgtcgcgc
    49381 tccttgcccg gactgtggct cgcgcagacg accagccgat cggatgccat gcgactaccg
    49441 cctctcgtca agtgacgcta atgttagatg ggcggccgaa gcaggtccag ttcgtgtcca
    49501 aaacggcgag aagagtccta tgaaattgcc atcgatggga cggcggcccg tcaagcgtcg
    49561 atgggccgcg gccattgggt attcattcgg cctcgtcgcc accgtcaacg gctatcggcc
    49621 gctggcgcgt aggggtttcc cctcggtctt tgccttcctc gcgggggtcg tcgcgacgga
    49681 gttgcctctg cagatgatcg ccgcgcaggc cgccggactg gcgtgcgttt cacgctggct
    49741 tccgaggcgc gccaggcgcg tcgcggtgct caccgcggct atgtcatgga tgggactgct
    49801 gggcctgcgc cgcatcggac atcgcgccga cacaccactg accgaggcac tggatatcgc
    49861 ccttggaacg gatcgtcgaa ccgagtcggc gggcctgtgg cggcatcctg acagactcga
    49921 tggaacagcg aaacagccag gtctggtgcg gatgctgcgc atctaccgcg actacgcgca
    49981 cgacgcagac attccgtatg gggatcacgg cgcggccaat cgcctcgaca tctggcgacg
    50041 tcgcgatctg gacaaagcag ggcgggcgcc ggtgctcatc caggtcccgg gtggtgcatg
    50101 ggcaacgggc aacaaacgag ggcaggccca ccctctgatg agccacctca ccgagctggg
    50161 ttggatctgt gtgtcgatca actaccggtt gagcccgcga aatacgtggc ccgatcacat
    50221 cgtcgacgtc aaacgcgcga tcgcctgggt gaaggagcat gtgggcgagt acggcggtga
    50281 cccgaacttc gtagcgatca ccggcggatc cgcgggaggc catctgagtg ccctgagtgc
    50341 cgtgaccgcc aacgacgcca ccctgcagcc agggttcgag gatgccgaca ccgccgtgca
    50401 ggcggccatc ccgtactacg gcgtctacga cttcactcgt accgatagag cgctacatcc
    50461 gatgatggtg ccgttcctgg agaggctggt cgtcaaacag ccgcggagag atgtccctgc
    50521 ggtgttcgca accgcgtcgc cgatcacgca tgccgattcc gacgcgccgc cgttcttcgt
    50581 gctgcacggc cgcaacgatt cgttcattcc cgtggagcag gcgcgtggct tcgtcgctcg
    50641 gctgcgtgag gtgagcaccc aaccggtcgc gtacgccgaa ctaccgttcg cccaacacgc
    50701 tttcgacacc ttcggctcgg cccgcgcggc gcatgccgca gtggccgtcg agcagttcct
    50761 ggccgagata tatgcgagac gcacgaccga cgtcgccccc cgcgccctgg cgctctaatc
    50821 ctctatggtg atcgcgccct cggggcagtt cgcggcgccg ttgtgcgctt gctcggcctg
    50881 cccctgcggc acctcaccgc tcccgatcac gtggccgtag ccttggtcgt catcctcgaa
    50941 tacgttgggc gccaatgagt aacacatgcc gtggcccatg cacttttcgg cgtcgattgc
    51001 agccttcact cgcgaccgcc tgtttccagg gtcaagtgca aggagtccaa gcccactgtt
    51061 ccccgtggcc agtgcacccg tggcgtcgtg cccggcgtga tgtggtagtt ggggatccgc
    51121 ttgtgccatt cctcgtatag cacctgcatc tcgaggcgtg cgagatgcga gccgaggcaa
    51181 cgatggaccc ccatgccaaa gctcatatgc cgattctcgg aacggtggaa gtcgatctcg
    51241 taagggttgg ggtacatcgt ctcgtcacga ttggccacgg ccaggtagct ggtgatccgg
    51301 ctgccctccg gcagcgtgtg accggccacc gtcacctcct cggtgctcac ccgtgggatg
    51361 aacggcgcag gcggatccag ccgcagcagt tcctccatgg cagccggaac gagcgtcggg
    51421 tcgttcacga tttcccgtcg cttgtccggg ttttcggcca gtcgttgcat tccgaacccc
    51481 agtgagtcca tcaccgtgtc cagtccggcg agcacgaaca agaagcacag gccgatggcc
    51541 tcttcatcgg aaagggcatc gggtggctcg atgctcagca cctgactgag cacgtcgtca
    51601 cccggagcac cgcgacgctt ctgaatcagc tcggagaggt atgtgaacag ttccagtgcc
    51661 ttcagcatgc cttcttgagc cgcttcgtcg accgatgtct gacccgccgc ggcgctgagg
    51721 ttgaggacgg cgtccttcca ttcgatgaac tgatcgcgca tgtccaacgg cagcccgaac
    51781 aacgtcagaa acacctgtat cggaaacacg gcggcgacct cggcgatgaa gtcacattct
    51841 ccgcgttgcg cgatcggctc gatgagatcg cggacctgct ggcgcagatc gggctccaag
    51901 ggcttgatca cgcgggggct gaagaacggc tgaaggatgc ggcggtagcg cgtctgctcg
    51961 ggtgggtcga acgcgatggg caccagcggg accgggctgg cgagcacatc gaacgcccgc
    52021 ttcgaggaga acgccgaggg gttcttgagg atcagttcca ccacatcgcg atgcgtggcc
    52081 gcatagccgt cctccacttc gacgaaaggg tgttctccca tctgatgcca agcgtcgtcg
    52141 cgggcctcag gcatcgggag ggcgtcggtg gacaccttgg gaagtgcgtc ggtggtcaat
    52201 ccgtcctcga ttcctactcg tgcgccgtgc gaacctgtga ccgcggttac tgcccgaagt
    52261 tttccattac cgtcagaaat tgacaacacc cccacggtgg tttctgcgcg acaagcaggt
    52321 tcgagcaatc gttacctgca catatacgtt catggtaggc atcattcgct gcaatattca
    52381 ggtgacgcgc ccgtcagact gtggtgatcc attgacactg tgacagtgaa ctgtcatctt
    52441 tggggtcttg atacgccgcc ggaaggcttc actgtgacag acctcaagag ctcagttcgc
    52501 gctgcgggtt cagggctacc ctcggctgcg cccgcagaga gcaaaacgct tcggcctgtt
    52561 ctcttttggt cggctgtcgg ggcggtctgc gtactgttcg ccgcctccgt ctacacctct
    52621 tggatcgtct cgggcaacgc ggttccggcg gatccgggac ccgatccgat ccctggcggc
    52681 actcagctcg cgatgaccgt cttccagatc gcctgtccga tattggctct ggtcgcgatc
    52741 gtgtacgtga tccgcaagag cgtgcgggaa cgtcagctct gcgtcgaggc cgccgtcgtc
    52801 atcgggtcgg cgatcgcgtg gtggcacgac cccctgatca actggtttca accggtgctg
    52861 ttctacaacg ccgggctggt caacttcagc aactggatgg agaacgtgcc cggctcgctg
    52921 agcccgggca gccggttcat ggccgagccc gtgttgatga tcggaatgat ctacatctgg
    52981 atgccgctcg ccatgggcag ggtcgcgaag tgggcgatgg gtcgggcgcg acgccagtgg
    53041 ccgacgctgg gacccgttcg cacgttctgc tgcggctggc ttgcggtgta cgtcatcgag
    53101 tttccgctgg agatcttcgc agtgcaccac ggtctggtcg cctatccggc gtcgatcccc
    53161 ggtgtcacgc tgtgggccgg gcagaccgta caaattccgc tgtacggccc cattctctgg
    53221 tcgttggtgc tcagctccag tggggcgttg atgttcttcc gcaaccagaa gggccagatc
    53281 cgcgtggagg cgggcgtcga gacgctgcgc cgggccggac cgcgagtcag gggcctgctg
    53341 cgcgtcctgg cggtcacggg cttcctgcac gtggtggcga tcggcatgta tgacctgccg
    53401 gtcaacctcg ccggtctcta tgcgggaccg accgacacct atccgacata tctgcgcacc
    53461 cagtactgcg gccctgacac gccacgggcc tgcccggacg gcgccctctt cgacgatccg
    53521 tagggcagct atttcaggac cgacggcaga gcggcgaact cctggcgtat cagatcatcg
    53581 agcgcgcggt tcgcgaccga cagccaccgg atcatcgcgc gctcgaccaa cagcattccg
    53641 atcgccgcca acaggcggca ctcatcgtcc ggcgacgcca gcctgcggcg ctcggcaatc
    53701 acgttcgcga cggtatctgc gttggcctgg tgattttggc gctcacgtcc ctgcagcaca
    53761 aaggatgacg cgacggcctc gtgatactgc gcgatccgct tgcggatgcg cttgaggccg
    53821 ggtgccagta acgcgaagga gtcggccatc gcctcgtaat ccgacagtgt ttcgtcctgg
    53881 gcgaggaaga cgccgctgaa cgcgttgatg aagccgtact cgccggagaa cagaaccgat
    53941 tccttggtag ggaagtagcg gaagaaagtg cgcggcgaca ctccagcggc gtcggcgatc
    54001 tcgtcggtcg tcgtctgttc atagccgttg gcgctgaaca gattcaaggc ggcctccagc
    54061 agtgccgtcc gcatcttgtc ggcatgttcg ctccgcggcg aacgatcgcc ccgcgccatc
    54121 gaacaacctc ctcggtcaca tcgtcgcagg atgcaattca accatccgaa tgtctactac
    54181 gtcgttgaca cccgttgccc cgcggacgat actcgccagt tatgacagtc tactgtcata
    54241 gtttgaatgc gcaatcgcct cgaagccgga aggaattccc gggtatggct gacacagccc
    54301 tgaacggaac cgccaacatc aacgacctgc cgtttgcgac ggatcggtcc cgcgcatggc
    54361 gggagctgcg tgaggccggg gaggcggtga gctccggcga ggaggtcgtc ttgaccagcg
    54421 cctcggcagt cgagttcgcg gcgaaaaagc ccgagatctt ttcttcggcg cgggccttcg
    54481 accgtctggg cagcccggtt ccacttgttc cgatcgccat tgatcctccg gatcacacgc
    54541 ggtttcgtcg catgctggat ccgttcttca gccccaagaa gatggccgaa cgggagccgg
    54601 agtttcgcag gcaggccggc gaactgatcg acgcgatcgt cgcaagggga gagtgtgacg
    54661 tggttgccga cctggcaaca ccgtttccgt cccaggtttt tctgacactc ttcggcctgc
    54721 cgatgtcgga ccgcgacagg ttggtgaaat ggaaggactc gatcctgcag ttcaccgacc
    54781 cgagcagcgc cgaagcaacc cccgaggtgc tcacccatgc gatggaactc ttcacctacc
    54841 tgaccgagca catcgccgag cgccgcaaca acaccacagg tagcgacatg ctcacccagc
    54901 tgatgcagaa caccgaggac ggcgggatgt ccgacaacga gatcctcggg ttgtgtttca
    54961 tgttcgtgct ggccggtctc gacaccgtga cctcggcggt gggattcgcg ctggccaaac
    55021 tggcgggtga ttctgatctt cgccatcgaa tcagtaacga ctacagcctg attccggctt
    55081 tcatcgaaga cttgctgcgc gtcgacggac ccgttccgtt cgctccccgg gtcacgaccg
    55141 aagacgtcga ggttgccggc aggctggttc cgaaggacac gacggtgatg ctcagctacg
    55201 gaagtgccga tcgcgacccg caacgctacc ctgacgctga caacgttcat ctggacagca
    55261 aggcagtgca tttcgcgttc ggacgtggtc cgcaccggtg cctggggtcg catctcgcac
    55321 ggctggaact gcggctcatt ctcgaagagt ggcacacgcg cattcccgaa tacacccttg
    55381 ccgacggcga cgaaccgcag atgccttggc ccacgggaac gatgggcctc aaatcggtgc
    55441 cgctgaaatt cagtgcctca tgatgcggcg gctcagtcga cttcggggat gacttcttct
    55501 gcgacccact gcatgtggtc gaggtgggag cggaggtagt ccattttcgc tggcaactcg
    55561 tcgggcttcg tcagccaggg tgcccagccg tcaccgaagc gcgcggcacg tcgcagcacc
    55621 gcgtcggcgt cgccgcccat ccagatgagg ggatgcggtt tgtgaatcgg cttggaccgc
    55681 ggtcaggccg tgctcccacg gttgagtgat cgcggcgagg tgcatgcagt tgggcaggtg
    55741 tacagcgaat ctcattatgt gccttccggg ttcggccgga cctgctgcga caagctatgg
    55801 cagtggactg ccacacgtca acgcctcagg gtcgggcact gtcggaacga cgtggcggcc
    55861 cggatcgctg ctgccgcgcg caggcgtaat actggcgctc ggcgttaact gggagttagt
    55921 cggtggcgtt gggcagtcgt tcgtcgtcag gcagcgggtc gccggttttc accgtgatgt
    55981 gcttgaagag aagccagcga ccgttctgtc ggcaaaggtg atcgtgatag cgcccgacaa
    56041 aggacatctc ggcagggcct gcctccggca ccagcgacac gatgacgtcg ctccatgctc
    56101 ttgcctcgtc tccgtcaatc tcgattaccg gtgcgtatac gaaatgtttg atccggtgcg
    56161 ggatctcggg ctgcgttcca ggcaacccct cgatgatttc ttttcgcccg cgatacgtct
    56221 ttccgcccca tggcagtaca ccgtcggtgc agaataacgc cgcaagttcg gtccatcgac
    56281 gttcatccag gagctgagcg tgcaatgcaa caacacgcct gatctcttcg taatcgtcca
    56341 ccgtcgcctc cttgaattcg tttgagttag tcaacttggg tcccgctcgt cgttcttcct
    56401 actgcccggg cagttgatca actatgtggg atttgtgccc agcgacgctg agacgcggga
    56461 atcctggtca gccggtctgc cagaagtgat cgccaatagc cgcaaatccc gtttccgata
    56521 catcggttta cggactctat cggcaatcag gatttaaacg acgtgctgcc gtagcaggcg
    56581 gcattgccca catcaagggt gctttcgcag atgaccttcc cgccaagata aatgcggacc
    56641 gttacccagt tgttgggcct ggcctccggg caccgggctc gcccatcaca cacgcgaggc
    56701 cgccagtcgg cgcgaacttc cgcgccatcg gttgacggat cgcgagcgtt gatcaccgtc
    56761 acactggttc gccatggcaa cggcaccgca gacaacactt ttcgctcaga ggagtcgaag
    56821 tactcgacag tcgcttcggc gacatcgttc gagacgacct cgtatgtaac ggtatcctgg
    56881 gcgtaagcgg acggggccaa cgcgatcgcg aagataccgg taaacaacgc aatcatcacg
    56941 agccggatgc gattcatatg agcccctcgt aggcgtcggg tcttgcggaa tgcggaattg
    57001 ctaggccccc gctaacaggt acacgaccag gagattatcg gtctcctcgt ttcgtcgtac
    57061 gtctattccc aggtccgtat aggagcaatc agggattgcg gcatggcctt gtatcaatgc
    57121 gccctttatc gcgtcagctt cgttgctcag ggcgccttga agcaaggcgg ccttattgcc
    57181 gccgtagccg agatccttca gcccgggcat tgggtctgtg atcggttggt atgtcgagag
    57241 atggtcgatg tagtcatcgg tcgactgatt gatgattccg gctgcttttt cgagtatcgg
    57301 gttgtagttc aacggctggc atgacgttcc ggcgcgggcg gtcgccatcg catcctgtac
    57361 gcgggttacc gaatcggcga gggctgacgg taccaggacc atgccaccgg aaaccagcag
    57421 cgcgcacacg gctaccggac ggatcgacct ccgccaggaa agtatcgacc tcgatctgcg
    57481 gcctgcgggt gaagcaggtg attgccttga cactgacaac tccttctcta agacatctcg
    57541 tcgtgcggtt tgatgccggt cagactcatc ggtagcaaga gagtttccgt caacaagacc
    57601 cgtagctcag gcacacacgc gtggag
//
LOCUS       DASYLX010001121        12912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3595396,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001121 DASYLX010000000
VERSION     DASYLX010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595396"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..587)
                     /locus_tag="VGP27_26680"
     CDS             complement(6..587)
                     /locus_tag="VGP27_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7854921.1"
                     /translation="MTATYTFDVFSSLDGFGGVSGGDWGGYWGKQGPELLDHRLALYD
                     EKQRMVFGANTYRAFAQMLAESTEDSGVRDPWVTRMRALPATVVSTTLDGSLDWPDAT
                     VVRGDAVDVVGRLKEESEVPLRSHGSLSMNRALMAAGLVDRVQVTLFPVITGQTGDDP
                     IFQGAADFDLELIESRTLDGNIQELIYRPALHV"
     gene            complement(741..1202)
                     /locus_tag="VGP27_26685"
     CDS             complement(741..1202)
                     /locus_tag="VGP27_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854922.1"
                     /translation="MSTIHFHRSTAASPEQFIAGLTDFGPGRSGLFPNSADGDLEVHH
                     QGACDADVTEGSGGIWERLHYDWSDPHRVTLTTTDSNTWAGASGYVYTLSPRPDDTTE
                     VDVVIVREGKTFKGRVLAAVLATIGKGVLRKAFVHSVKAIETRSDAPTSAS"
     gene            complement(1516..2118)
                     /locus_tag="VGP27_26690"
     CDS             complement(1516..2118)
                     /locus_tag="VGP27_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV7854923.1"
                     /translation="MTTIETPSVVRNLLNSGLVLGALNLVLGVAVLIWPGDSIVVASV
                     VFGAYLLVSGIAQVIAAFAVDMSLGSRVLFLLSGALSVVLGYLAFRDFNNGTAVWMLA
                     IWIGVGFIMQGVAATVLAIDFPALPQRGWYIFVGILTVIAGVVTLVWPISSIVVLAIT
                     AGTWLVFIGITQVVWAVSARRTVKEGERRIQESWAPTTAS"
     gene            complement(2124..2552)
                     /locus_tag="VGP27_26695"
     CDS             complement(2124..2552)
                     /locus_tag="VGP27_26695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854924.1"
                     /translation="MQKLSAALLDLAGRAKRVEDSAAAVEAKNRVKLQERRGELEAAI
                     DESHIELAAAATQAKEAANGKWSAVKNSIDGRVDEMRSEFANWQDEIKEDRAERAAED
                     AEYDAVVAVDLAVYCLDAAEWAVVQAELARGEADQLAARR"
     gene            2761..3600
                     /locus_tag="VGP27_26700"
     CDS             2761..3600
                     /locus_tag="VGP27_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7854925.1"
                     /translation="MATNANALGDYLRAKREQLRPDDVGLVPGSRRRVPGLRREEVAT
                     LAGISAAYYLRLEQGHDTCPSAQVVEALALALRLDRKGAEYVHRLASSTGSPPQSTVQ
                     SVTQRLRELVDQMLFPAMVLNRYLDVLAANPCARAMTPGAAPGRNRMRDVFLDPATRE
                     RWVNWDELTDIAVCEFREAAGDLDDPRFRALFDELSAGSERFRDLWARADVGYSTGIV
                     HMRHPDVGELRLHRTRLDVPHSGGQLLLMYHAEPGSESAKGLEALRSQGSRFSSAEPS
                     GTN"
     gene            3779..4096
                     /locus_tag="VGP27_26705"
                     /pseudo
     CDS             3779..4096
                     /locus_tag="VGP27_26705"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
     gene            4520..6055
                     /locus_tag="VGP27_26710"
     CDS             4520..6055
                     /locus_tag="VGP27_26710"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7854926.1"
                     /translation="MTRASKPETAASGCLPEHVQIAIVGSGFSGVGAAIKLDEAGHRD
                     FVVLERGEDVGGTWRDNTYPGAACDVPSHLYSYSFALNPDWTRSFSTQPEIQEYIRGV
                     ARRSGVLDRHRFGCEVKRAAWQQHRNRWELSTSHGTMTADVLIGAFGALAEPSAPSIP
                     GIDDFDGELFHSAQWNHGADLTGKRVAVIGTGASAIQLVPAIADTVAHIDVYQRTAPW
                     LLPRFDRAFTRVERWAFRNIPAALRLARTGIYAARETQVVGLAKNPRLMKPFELISRA
                     KIRTEIKDPELRRKVTPDFRIGCKRMLIANDWYPTLDRAHVELVTDGISEVDGRRIVS
                     KDGTAREVDALIVATGFHVTDSPMFDVVCGSDGRSLSQTFADKGMRAFKGTTIAGFPN
                     MFVLVGPNVGLGHTSMVYMIESQIHYVVDALTTMKRRGIARVDVRRDVQDAYNDELQR
                     KLGGSVWMTGGCASWYLDAHGNNTTLWPDFTFRFRRQTKRFDIDAYETAPVADELVRK
                     GNG"
     gene            6055..6906
                     /locus_tag="VGP27_26715"
     CDS             6055..6906
                     /locus_tag="VGP27_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854927.1"
                     /translation="MSSFDGKVAVVTGAGSGIGRQLAVELARRGAHLAISDVDEEGLK
                     GTAERLAALNAKAHAAVLDVSDCSAVQTYASSVVAHYGVVHQVYNNAGVAGGAAPVVD
                     CDYDAYERIIAINLWGVIHGTKEFLPYLIESGDGHVVNISSLNGIMAQASMSAYCTTK
                     FAVRGFTESLRAEMLSAGHPVRVTLVHPGGIKTNIATAAITEAERTGAQITEQQRQRA
                     DAYNDKLLKMSPQQAARIILAGVQANRSRVLVGKDAKAVDALVRLAPRYYSRIVMWWD
                     KRTFGGP"
     gene            complement(6926..8746)
                     /locus_tag="VGP27_26720"
     CDS             complement(6926..8746)
                     /locus_tag="VGP27_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7854928.1"
                     /translation="MSIDSRSQSTATSSTESKNLATMFYDRVEASGDSEAFRRLVDGK
                     WTSLTWQQAAEQVEALAAGLLALGLEAEQRIGIASSTRYEWVLADLAIMCAGGATTTV
                     YPTTNANDTAYILHDSGSRFVFAEDEAQLQKLRERRDDLPDLEKVVLFDGAGDGDWVL
                     TLDDVVAMGNKHLQEHPRSVRDRADGIPGEQLATLIYTSGTTGKPKGVRLSHRAWVYE
                     GATIAGFGILDDDDLQLLWLPLAHAFGKVLISAQLACGFASAIDGRVDKIVENMGTVK
                     PTFMGAAPRIFEKAHAKVVTSQKGAKEKLFNAAFGVGRQVDRRRLAGKSVPLPLKAAH
                     GLFDRLVFSKVREVFGGRIRFMISGSAPLNREIGEWFHAAGLLILEGYGLTETSAGAF
                     LNRMEHYKLGTVGQVFDGTEVRISDDGEVLVKGPCVMDGYHNLPDKTAETLTEDGWLR
                     TGDKGQLDDDGFLTITGRLKELFKTSGGKYVAPPAIESKFMALCPYAGHMLVFGESRN
                     FCVALITLDPDAISGWAKENGLGDQDYADLAKSDRVRDMIGEHVQRLNSELNRWETIK
                     KWEILDHDLSIERGELTPSLKVKRAVIADEQKELLDSFYS"
     gene            9037..9354
                     /locus_tag="VGP27_26725"
     CDS             9037..9354
                     /locus_tag="VGP27_26725"
                     /inference="COORDINATES: protein motif:HMM:NF014479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEV7854929.1"
                     /translation="MQYFVQFDVHQPVDMSREELFTIWLEEARAAQGAVDAGAVTNLW
                     KVVGQRSVFAVLDVPDHTSLDQALESLPIIQKLGSSVDTRAYPIRPYSEWAEELRVAV
                     EGD"
     gene            9374..9814
                     /locus_tag="VGP27_26730"
     CDS             9374..9814
                     /locus_tag="VGP27_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV7854930.1"
                     /translation="MSWNSLRDNGAKLMNLAHRLVLTASGNRLLAKPFGMPLVELHTT
                     GRKSGLPRSCYLTTPVHDSERVVLIASKGGDDRHPDWYRNLQARPDTFLVINGQRHAV
                     HARTASAEEKAELWPKIVASYKGYADYQQRTDRDIPVVICELQN"
     gene            10039..10560
                     /locus_tag="VGP27_26735"
                     /pseudo
     CDS             10039..10560
                     /locus_tag="VGP27_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            10570..11025
                     /locus_tag="VGP27_26740"
     CDS             10570..11025
                     /locus_tag="VGP27_26740"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEV7854931.1"
                     /translation="MSHSAPHVREKHTDTRQQWEGKGEPIGDWFTEPETADQPSLVRP
                     YTLTAGHTDPGFELPLDAPVEALNTTMKPPRWPRNDVRGQILTCCVHSLSVAEIAAHL
                     SLPLGATRFLVGDLVTQGYLRVHAISGDSMTIDERRELIRRTLVGLRAL"
     gene            complement(11155..11493)
                     /locus_tag="VGP27_26745"
     CDS             complement(11155..11493)
                     /locus_tag="VGP27_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEV7854932.1"
                     /translation="MTVSAETSIRYHPEARNAWVASARCRTTDPDELFVSGAAQRKAA
                     VICRHCPVMTECAADALDNRIEFGVWGGLTERQRRVLLKKHPDVDSWAEFFGARREHR
                     DAGLLTTRVS"
     gene            12327..12740
                     /locus_tag="VGP27_26750"
     CDS             12327..12740
                     /locus_tag="VGP27_26750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854933.1"
                     /translation="MASDNVVQVRFGAPGNVLEVRVGTVEVDVPRSVGYFGGLAVAVA
                     VGLIEPPLALFIAAVPVFKSLTNTALPKAVRLVGEVLEGAAKPVGSDAEGVIELKDQH
                     GSDTKVVNVAPKATRRRAPKGAPRRAAPRRASVNT"
BASE COUNT         2433 a         4115 c         3976 g         2388 t
ORIGIN      
        1 tggaatcaga catgcagggc gggccggtag atgagctctt ggatattgcc gtcgagcgtt
       61 cggctctcga tcagctcgag gtcgaagtcg gccgcaccct gaaagatcgg gtcatcaccg
      121 gtctgaccgg tgatcacggg gaagagcgtc acctgaacgc ggtcgaccag gccggcggcc
      181 atcagcgccc ggttcatcga caggctgccg tgcgagcgca acggcacctc ggactcttcc
      241 ttgagccggc cgacgacgtc gacggcgtcg cccctcacga cggtggcgtc aggccagtcg
      301 agagacccgt ccagggtcgt cgacaccacc gtcgccggca gggccctcat ccgggtgacc
      361 cacgggtcgc gcaccccgga gtcctcggtg ctctcggcca gcatttgcgc gaatgcccgg
      421 tatgtgttgg cgccgaagac cattcgctgc ttctcgtcgt agagggcgag gcggtggtcg
      481 agcagttcgg ggccctgctt gccccagtag ccgccccagt cgccgccgct gacgccgccg
      541 aagccgtcga ggctggaaaa gacgtcgaag gtgtaggtag cggtcataat gctctcctcg
      601 ggtgcggttg ggggtgagtt ggggatacag actggaagcc atggcgaaac taatcgcgtc
      661 tgccgatcca caggtcctga ctattcctcc agcgcaccga gaccaccccg ccgcgggcaa
      721 tcggtgatcg gatgtttccg tcaacttgcg ctggtcgggg cgtcgctgcg tgtttcgatg
      781 gctttgacgc tgtggacgaa tgccttccgt agtacgccct tgccgatcgt tgcgagtacg
      841 gccgccagga cgcgtccctt gaaggtcttg ccctcgcgga cgatgacaac gtccacctcc
      901 gtggtgtcat cgggacgcgg gctgagggtg tagacgtagc ccgatgcccc ggcccaggtg
      961 tttgagtcgg tggttgtcag cgtgacgcgg tggggatcgg accagtcgta gtgcaggcgt
     1021 tcccagatgc cgccggagcc ttcggtgaca tcggcgtcac acgcgccctg gtggtgcact
     1081 tcgaggtcgc cgtcggcgct attgggaaac agcccggagc ggcccggtcc gaagtcggtg
     1141 agcccggcga tgaactgctc agggctggcg gctgtcgagc ggtggaagtg aatcgtggac
     1201 atggtgtcct ccttgtgtga cggagttggt cttcgtccac gggaagcgcc tcgccgccag
     1261 cgcgaattcc cgcttggacc tcggcgggga tgccttgttg atcctggtat tggcagggac
     1321 agccttagga tcgggcaatc tctatcttcg ccgcaccagg ctcaccgtcc ccgactccga
     1381 cactcgggtg ttcatatacc accccgaacc gggcggtgat tcggcaaagg ccactgaagg
     1441 cactcgcagc ctcagtcgca acaaagctcg gtgtgctgag acgcccaatt gcgcctcagc
     1501 acaccaagag gtgagtcagc tggcagtagt gggcgcccac gactcctgga tacggcgctc
     1561 cccttccttg actgtccgac gtgcgctcac ggcccacaca acttgtgtga tcccgatgaa
     1621 gacgagccag gttccagcgg tgatcgccag caccacaatg gagctgatcg gccagacaag
     1681 cgtcacgacg cccgcgatga cggtcaggat cccgacaaag atgtaccagc cgcgctgggg
     1741 caatgctggg aaatcgattg ccagcacggt cgccgcgaca ccttgcatga tgaagccaac
     1801 gccgatccag atggccagca tccacacggc agtgccgttg ttgaagtcgc ggaaggccaa
     1861 gtagcccaac acgaccgaca gcgcgccgct gagcaagaac agaacgcgac taccgaggga
     1921 catgtcgact gcgaacgccg cgataacttg cgcgatgcct gacaccagca ggtacgcacc
     1981 gaacaccact gacgcgacga cgatggagtc accgggccag atcagcacgg cgacacccaa
     2041 gacgaggttc agcgcaccta gcaccaaccc ggaattcagt aggttgcgca cgacggatgg
     2101 ggtttcgatg gtggtcattt cccctacctc cgtgccgcga gttgatcggc ctcgccacgg
     2161 gcgagctcgg cttgaaccac cgcccactcc gcggcgtcga ggcagtagac cgcgaggtcc
     2221 accgcgacga ccgcgtcgta ttcggcgtcc tcggccgccc gctctgcgcg atcttccttg
     2281 atctcgtcct gccagttagc gaattcggaa cgcatctcat cgacccgtcc gtcaatcgag
     2341 ttcttgaccg ccgaccactt tccgttcgct gcttccttag cctgcgtcgc ggctgcagcc
     2401 agttcgatgt gcgactcgtc gatcgctgcc tcgagctctc cgcggcgttc ttgcaatttg
     2461 acacggttct tcgcctcgac cgccgcggcc gaatcttcga cccgcttggc ccggccggca
     2521 agatccagca gggccgctga caacttctgc atttgcttct cctttaggtg tgttgccgaa
     2581 cgtttccgtg cagcaagggt tttcattggt tgtgtccgca gaatggttag ctgcgttagc
     2641 tatccgcccg gcatctacga tcggcaggag cgcacttgat gccttgggaa ttcctcggga
     2701 tagacggcag cctgaccgaa gtcatcgtga tattggcacg gacagcctta cgatcgggtg
     2761 gtggcgacta acgccaacgc gttgggtgat taccttcgag cgaagcgcga gcagcttcgt
     2821 ccggacgacg tcgggctagt accaggctcg cgccgccgcg tgccgggttt gcgccgcgag
     2881 gaggtggcga cgctggccgg tatcagcgcg gcctactacc tgcgcctcga gcagggccat
     2941 gacacgtgtc catcggcgca ggtcgtggag gccctcgcgc tggccctgcg gctcgatcgc
     3001 aagggggccg aatacgtgca tcggctggcg agctcgaccg gcagcccccc tcagtccacc
     3061 gtgcagtcag tcacgcaacg gttgcgcgaa ctggtcgatc agatgctttt tccggccatg
     3121 gtgttaaaca gatacttgga tgtgctggcc gccaatccat gcgcccgcgc catgacgccg
     3181 ggagccgcac ccggccgcaa ccgcatgcgc gacgtgttcc tcgacccggc cacgcgggag
     3241 cggtgggtca actgggacga gttgaccgac atcgcggtgt gcgagtttcg cgaagccgca
     3301 ggcgatctgg acgatccgcg gttccgcgca ctgttcgacg agctgtcagc cggcagcgaa
     3361 cgctttcgtg acctatgggc gcgcgcggac gtcggctact ccacaggcat cgtccacatg
     3421 cgtcacccag acgtgggtga actccgtctc caccggaccc ggctcgacgt ccctcactcg
     3481 ggtggccagc tactgctgat gtatcacgcc gaacctggca gcgagtcagc caaagggcta
     3541 gaggcacttc gatcccaagg cagtcgtttc tcgagtgcgg agccctcggg aacgaattga
     3601 gattccgcca aaccaggctg acaccatcaa accgccccgg cgccgcgacg gatgccgacc
     3661 tgacggcggc gtacgcctac gatgcgatcg atttcgcgct tggacacggt cgaagaggca
     3721 tacgctttgc ttgacgccat gtatgcaaaa aaggcgggcg gcggcactcg gaatttgatt
     3781 gaagccaacg gtccaagagc gacgcgacct gggcagaggc cggcgatcgg cgctgcccag
     3841 gtcggcgctc gcagatttcg tcgtagtacc ggatagacct gatccgatcg agcttcttga
     3901 gtcacaatcg gggtcgcgta ttcccgacct ggcgccaata cgatacgtgc ggatgttgca
     3961 atcgcagttc gcattcttcc gcggcgcggc gctgatcatg gcgtccgact tggcctctac
     4021 cccgcgtacc ggttttaccg tgcaattgtc cggcgacgcg cacgtgtcca acttcggatt
     4081 gttcgcctcg cccgagaaaa acctagcatc acaatcgcgg gctatctcgg tagcggtgac
     4141 gccgccgacg tcgcgttcac ccagttcgcc gagacgtacg ccctacagaa cgagcgtgat
     4201 tacgaggccc tgcgcgaggc cgtcgccgac ggtcgcgttg ccgccgcgcc tagcttctga
     4261 caacagtgat tccgcgcctt cgagattcgt cagctggtcg tcgaaccacg cactgacgtt
     4321 ccgcgacttg tcgttgacat ttcccggcgc ggttgcatct gcgcaaatcc gcgaaccccg
     4381 acctctgccg ggttcggagg ccagcccgaa tcgtctgttc gcattttccg gttcggccca
     4441 actactagtg ggtaacctac tgcaccgtag gttctggagc gcccaccctg agggcgcaca
     4501 cggccaccgg gagaaactca tgactcgcgc ttcgaagcct gaaactgctg catcggggtg
     4561 cctgcctgag cacgttcaaa tcgcgatcgt cgggagtggg ttctccggtg tgggtgctgc
     4621 catcaagctc gacgaggcag gacaccgcga cttcgtcgta ttggaacgcg gtgaggacgt
     4681 cggcggcacg tggcgcgaca acacctaccc cggcgcggcc tgcgatgtgc catcgcatct
     4741 ctactcgtac tccttcgcac tcaaccccga ctggacccga tcgttttcaa cccaacccga
     4801 gatccaggaa tacatccgcg gcgtcgcgcg ccgctcaggt gtcctcgatc gacacaggtt
     4861 cggatgcgaa gtgaaaaggg cggcttggca acagcatcgc aaccggtggg agctgtcgac
     4921 ctcgcacgga acgatgaccg ccgacgtttt gatcggcgcc ttcggcgcgt tggccgaacc
     4981 gtcggcgccc agcatccccg gcatcgacga cttcgacggc gaactgttcc actcggcgca
     5041 gtggaaccac ggcgcagacc tgaccggcaa gcgcgtcgcc gtcatcggca ctggtgcgtc
     5101 ggcgatccaa ctcgtgccgg ccatcgccga caccgtggca cacattgacg tgtaccaacg
     5161 caccgcccct tggctgctgc cgcgcttcga tcgcgcattc actcgcgtcg agcggtgggc
     5221 gtttcgcaac attcctgcgg cgctgcgtct ggcccgcacc ggcatttacg ctgcgcggga
     5281 gactcaggtg gtcggactgg ccaaaaaccc gcgcttgatg aagccattcg agttgatttc
     5341 acgcgccaag atccgcaccg agatcaagga cccagaacta cggcgcaagg tgacaccgga
     5401 cttccgcatc ggttgcaagc gcatgctcat cgcgaacgac tggtatccca cactggatcg
     5461 cgcgcatgtc gaattggtca ccgacggtat cagcgaggtc gacgggcgtc ggatcgtctc
     5521 caaagatggc accgcacgtg aggtcgacgc cctcatcgtc gcaacgggtt tccacgtcac
     5581 agactccccg atgttcgatg tcgtgtgcgg cagcgacggc cgaagcctca gccaaacgtt
     5641 cgccgacaag ggcatgcgcg ctttcaaagg cacgaccatt gcgggatttc cgaatatgtt
     5701 tgtgctcgtc ggccctaacg tcggcctggg tcacacctcg atggtgtaca tgatcgaatc
     5761 ccaaatccac tacgtcgtag acgccctcac caccatgaag cgtcgcggca tcgctcgcgt
     5821 cgacgtccgc cgggatgtcc aagacgcgta caacgatgaa cttcaacgca aactcggtgg
     5881 atcggtgtgg atgaccggcg gctgcgccag ctggtatctg gacgctcacg gtaacaacac
     5941 caccctgtgg cccgacttca cgttccgctt ccgccggcag accaagcgct tcgacatcga
     6001 cgcatacgag actgctccag tcgcagacga gttggttcgt aagggcaacg gctgatgagt
     6061 tcgtttgacg gcaaggtagc ggttgtcacc ggtgccggtt cgggcatcgg acgacaattg
     6121 gctgtcgaat tggcacggcg tggggcccat ttggcgatct cagatgtcga tgaagagggc
     6181 ctgaagggga ccgctgaacg ccttgccgcg ctcaacgcaa aggcacatgc ggcggttctc
     6241 gacgtcagcg actgctccgc ggtacaaacc tacgcctcgt cggtggtcgc gcattacggt
     6301 gtcgtgcacc aggtctacaa caacgccggc gtcgccggag gcgcggcgcc cgtcgtcgac
     6361 tgcgactacg acgcttacga gcggatcatc gcgatcaact tgtggggtgt tattcacggc
     6421 accaaagagt tcctgccgta tctcatcgag tccggcgacg gacatgtcgt caacatctcg
     6481 agtctgaacg ggatcatggc acaagcgtcg atgagtgcgt actgcacaac caagttcgct
     6541 gtccgaggat tcactgaatc gcttcgcgcg gagatgcttt cggctggcca cccggtgcgc
     6601 gtcacactgg tgcacccggg tggcatcaag accaacattg ccacagccgc gatcaccgag
     6661 gccgagagga ccggagcgca gatcaccgaa caacaacggc aacgcgcaga cgcctacaac
     6721 gacaagctgc tcaaaatgtc gccgcagcag gcggcccgca tcatcctcgc cggagtacaa
     6781 gccaaccgca gccgcgtgtt agtgggcaag gatgccaaag ccgtcgacgc cttggtgcgg
     6841 ctagcgcctc gctactacag caggatcgtc atgtggtggg acaaacgtac attcggcggg
     6901 ccatgaggcg ggcaaccagt gcgggtcagg agtaaaacga gtcgagcagt tccttttgct
     6961 cgtcggcgat caccgcccgc ttcaccttca acgagggagt cagctcgccg cgttcgatgg
     7021 acaggtcgtg atcgaggatc tcccacttct tgatggtctc ccaccggttc agctcggagt
     7081 tgagtcgctg cacgtgctcg ccgatcatgt ctcgtacccg gtcggacttc gccagatcgg
     7141 cgtagtcctg atcgcccagc ccattctcct tggcccagcc gctgatcgcg tcgggatcca
     7201 gcgtgatcag cgcgacgcag aaattgcgtg actcgccgaa taccagcatg tggcccgcgt
     7261 agggacacaa cgccatgaac ttgctctcga tcgccggcgg tgcgacgtac ttgccgccgg
     7321 aggtcttgaa cagttccttg agccggcccg tgattgtgag gaagccgtcg tcgtcgagct
     7381 gacccttgtc gccagtgcgc agccagccgt cttcggtcag cgtctccgct gtcttgtcgg
     7441 ggaggttgtg gtagccgtcc atcacacagg gacccttgac cagcacctcg ccgtcgtcgc
     7501 tgatacgcac ctcggtgccg tcgaagacct gcccgacggt gccgagcttg tagtgctcca
     7561 tgcggttgag gaaggcccca gccgacgttt cggtgaggcc gtaaccctcc aagatcagta
     7621 gacccgcggc gtggaaccat tcgccgatct cgcggttcag cggcgctgag ccggagatca
     7681 tgaacctgat ccggccaccg aacacctcgc gcaccttgct gaacaccaac cggtcgaaca
     7741 acccatgcgc ggccttcagc ggcagcggca ccgacttgcc cgccagccgc cggcgatcca
     7801 cctgccgccc caccccgaac gcggcgttga acaacttctc tttggcaccc ttctgagagg
     7861 tgacgacctt ggcgtgcgcc ttctcgaaga tgcgcggggc ggcgcccatg aacgtcggct
     7921 tgaccgtgcc catgttctcc acgatcttgt ccacccggcc gtcgatggcg ctggcgaacc
     7981 cgcatgccag ctgggccgag atcagcacct tgccgaacgc gtgggccagc gggagccaca
     8041 gcagctggag atcgtcgtcg tcgaggatgc cgaagccggc gatagtggcg ccctcgtaca
     8101 cccaagcgcg gtgcgacaat ctgacaccct tgggcttgcc ggtggtgccc gacgtgtaga
     8161 tcagtgtcgc gagctgctcg ccgggaatac cgtcggcgcg gtcgcgcacc gacctaggat
     8221 gctcctgcag gtgtttgttt cccatcgcca cgacgtcgtc gagtgtcaac acccagtcgc
     8281 cgtcaccagc accgtcgaaa agcaccacct tctcgaggtc cggcagatcg tcgcggcgct
     8341 cccgcagttt ctgcagctgt gcctcgtcct cggcgaacac gaaccgcgac cccgagtcgt
     8401 gcaggatgta ggcggtgtca ttggcgttcg tcgtcggata aacggtggta gttgcgccgc
     8461 cggcgcacat gatcgcgagg tcggccagga cccactcgta acgggtactc gacgcgatgc
     8521 cgatccgctg ttcggcttcg aggcccagcg ccagcagccc ggccgccagt gcttccacct
     8581 gctcggcggc ctgctgccag gtcagcgagg tccacttccc atcgacgagt cttcggaacg
     8641 cctcgctgtc tccggatgcc tcgacccggt cgtagaacat cgtggccagg ttcttggatt
     8701 ccgtactgga agttgcggtg gactgggagc ggctgtcgat ggacatcgtc atctttcgcg
     8761 ttcgagggcg gaaggtgtgt tcccggccac aaacgtttct acactccgtc gaccgcgagg
     8821 ctggagaagg ctttcgctgc cctccgccaa gacgaccttc atccgtttcg cactggcatc
     8881 cgaggccaag acgccgtagt cagcaccgct caacgaatcg ccgcgcatgc aaccccgact
     8941 tcgcacagaa ggcttcaacg tcgttcactc attccgagcg acgtgagtgg tcgtgggaag
     9001 cttttcccgc ctaacagcga agttaaggag gagatggtgc agtacttcgt ccagtttgac
     9061 gtccaccagc cggtcgacat gagccgcgaa gagttattca cgatctggct cgaggaagcg
     9121 cgcgcggcac agggtgcggt cgatgccgga gcggtaacca atctgtggaa agtcgtcggc
     9181 cagcgcagcg tcttcgccgt gctcgacgta ccggaccaca catcgctcga ccaggcgctc
     9241 gagtcgctcc cgatcattca gaagctcggc agctcggtgg acacgcgcgc ttatccgatc
     9301 cggccttaca gcgaatgggc cgaagagctg cgcgtcgcgg ttgagggcga ctaactatcc
     9361 gaaaggactt gtgatgagtt ggaattcgct aagagataac ggcgcgaagc tgatgaacct
     9421 tgcgcaccgg ctggtcctca cagctagcgg gaacagacta ttggccaaac cgttcgggat
     9481 gccgttggtc gagctccaca ccacggggcg caagtcgggg ttgccacgat cctgctatct
     9541 gaccacgcct gttcacgact ccgaacgggt ggtgctcatc gcatcgaagg gcggggatga
     9601 ccgccaccct gactggtacc gcaatctcca ggcacgccct gacacatttc tggtgatcaa
     9661 cggccaacgg cacgccgtcc atgcgcgtac cgcaagcgca gaagaaaagg ccgagttatg
     9721 gcccaagatc gttgcgtcat ataagggtta cgccgactat cagcaacgaa cggatcgtga
     9781 cattccagtg gtgatatgcg agttgcagaa ctgaggaccc tcgacgatcg ccgtgtcaac
     9841 ggcatagtgt gccgatctga cctagtccaa ggcatccgtg ctcagcacat cgacagggac
     9901 cgtaacccac agtggtcgcc aattccccgc atcctcggtg tggccgagcc gatcaggttc
     9961 ccacctggca gctgttgggc gatgaccgag ttacccgagt cgtgcgtcgc gcagagcttt
    10021 ccgcgttagt ctttgccgat gatcgcgggc gacaatgcgc ctgacttcac tctgtacgac
    10081 cacaccggtc ggcctcggac actgtctgcg ctcctttccg atgggccggt cgtgctgttc
    10141 ttcttcccaa tagcgtcctc accgatctgt accgcccagg cttgccattt tcgggacctg
    10201 accagcgaat tcgccgcggt gggcgcccag cgcgtgggca tcagcaccga caccgtcgac
    10261 aagcaggccc acttcgccca acagcgctcg ttcgactatc cgctgctctc cgacgcggac
    10321 ggcgtggtgt ccgagctgtt cggggtgcgc cgaggcaggc tcgccaagct gcgtaaatcc
    10381 gtcgtggcgc gtgaggcggc ccggcgccgc cggcacactc gacgccgtgg cctgctggcc
    10441 cggctgatgc cggtcagacg gaccacattc gtcatcgaca ccgaccgcac tgtactcaag
    10501 gtcgtctcca gcgagctgcg cgcatcggtg cacgctgacc agaccttgcg gttcttgcag
    10561 aacctcaacg tgtcgcacag tgcgccacat gtccgggaga agcacaccga cacgcgtcag
    10621 cagtgggagg gcaagggcga accgatcggc gactggttca cagaaccgga aaccgcggat
    10681 cagccaagcc tggtccggcc atacacgttg acggcaggcc ataccgaccc tggtttcgaa
    10741 ctgccactag acgctccggt cgaagcgctc aacaccacaa tgaagccgcc gcgctggccg
    10801 agaaatgatg tgcggggcca gatcctgacg tgttgcgtgc acagcctctc ggtcgcagag
    10861 atcgccgctc atctgtcctt gccactcggt gcgacgcgtt tcctggtcgg cgacctggtg
    10921 acacagggtt atttgcgggt gcacgccatc tccggcgact cgatgacaat cgacgaacga
    10981 cgtgaactga taagaaggac cctggtcggc ctacgagcac tctgaactgc ggatttaagt
    11041 ggagctaagg ggactcgagc ccactggata acgccccatc caatcgtctg gtcagactcg
    11101 tcgcatcaac acgacgcagg ggcgtgccgg cgctttggtg tcagccgatc gttgtcagct
    11161 gacacgcgta gtcagcaagc cggcatctcg gtgttctcgt cgggcgccga aaaactcggc
    11221 ccaggaatcg acatcagggt gcttcttcag cagaacgcgt cgttggcgtt cggtcaaccc
    11281 accccagaca ccgaattcga tgcggttatc gagtgcgtcg gcggcgcact cggtcatgac
    11341 cggacagtgc cggcagatca cggccgcctt tctctgcgcc gctccgctaa cgaacaattc
    11401 gtcggggtca gtcgtgcgac agcgcgcact ggcaacccag gcgttgcggg cttcggggtg
    11461 gtatcgaata gaagtttctg cactaactgt catggccgcc ccctgcgcgt tgtcgttgtg
    11521 tgccccagca acccgcagca accgaacggc gggaatgcgt tgaccaaaag tctaggtgaa
    11581 cctacgggta tagcaaatca tgcggcggtc agctcttcaa gatgattgcg aatgcaatta
    11641 gctcactgtg gcaaggatgg actgaaccga taaaggcgga gctcacaacg ctgtgctgcg
    11701 ccaagacacg ttgactggca aacaaaattt gctggccgac gacctggcat ttgccgcccg
    11761 ggtgtggccg aaagcgatga gacagctacc ctagagctgc cggaaaaaaa cctgctgaat
    11821 gacaaggtaa ggccgagtcg gcgtcatgtc cgcaactact agcgcagcca acacaaacgc
    11881 cccgaccgat agccggggcg acctctaccg atgacgcgac tcacgtgaat ctcataactc
    11941 gactcatcaa atgagtggag ctagtgggaa atgagtggag ttaagggtgt gtgaatgcac
    12001 gacatgggtg acaggcccgc ggagtcgacg tcgcgaagcg tttcctcggc cacgtcccgc
    12061 tgatcgctcg tcgtacagaa gatttccgag cggtagttaa agccgacgag atcctcgtcg
    12121 aggtcgggtc ggtggaccga gaagaaccac tcgagcagct cacgatgccg ccactttcgc
    12181 ccaatcagtg caccaacatc ggctacctga aggtgctgag ttgaccgtgc ccgatgcggg
    12241 tatacaagcg gatccggccg agcggcgacg ccggcgccgg gcagcgcgaa ctttagtagc
    12301 gcgaacggta gcaaatggag gacgcgatgg cttccgacaa tgtggtgcag gttcggttcg
    12361 gtgcgcctgg caatgttctg gaggttcggg tcggcacggt agaggtggac gtgcctaggt
    12421 cggtggggta tttcggtggc ctcgcggtcg cggtggcggt tggcctgatc gagccgccgc
    12481 tggcgctgtt catcgcagcg gtaccggtct ttaagtcgtt gaccaacacc gccttaccca
    12541 aggcggtgcg cctagtcggt gaggtgcttg aaggcgccgc caaaccggtg gggagcgacg
    12601 ccgagggagt catcgaactc aaagaccaac acgggtccga tacaaaagtc gtcaatgtgg
    12661 caccgaaagc caccaggcgg cgcgcaccga aaggcgctcc gcggcgagca gcacctcgtc
    12721 gcgcaagcgt aaacacctaa gctcgctgcg cagcggtggg tcccggttcc tagcgcaacc
    12781 gtgctgggcc cctcgatccg tgtcggggat gttctccgag tgctagtgac acgttcaact
    12841 aacgctcggc ttgacccgac ccgcacggcg ggcgcaacct gcttacaacg ccgtccgcac
    12901 gtctcgactc ct
//
LOCUS       DASYLX010001122         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2170798,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001122 DASYLX010000000
VERSION     DASYLX010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170798"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VGP27_26755"
     CDS             complement(<1..570)
                     /locus_tag="VGP27_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7854934.1"
                     /translation="MTESLVATGCRALLRSRLLIPPGPAALPRFVREVVRGRANLVTL
                     LGMAAARWPHRTAMIDDEGAISYRELLWCTDSLADQLQRRGSGPGHAVGVLCRNGRAF
                     AAAVFAAATVGADVVLLNTDFRTESLAAALRSHRIGLVVCESEFASRVRDADDGVRVV
                     DAADVDMREGSRPAVARAGRIVLLTSGTTG"
     gene            complement(567..1448)
                     /locus_tag="VGP27_26760"
     CDS             complement(567..1448)
                     /locus_tag="VGP27_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7854935.1"
                     /translation="MSLATKALDKVVNPSHGSAPDRLRDAVSGKTVLVTGASFGIGEA
                     TARTLAAAGATVLLAARSEDKLDELVRAIDAGGGRAVSYPADLSDEAAATALAQRITD
                     EHGPPDIVVSNAGKSIRRSLHHQYERPHDFHRTIGINYLGPIWLLLGLLPAMRERGTG
                     QIVNVSSVGVRVPPGPRWGAYQASKGAFDMWLRSVAAELHADGIDVTSIYMALIHTRM
                     TAPTESLRNAPGLTPDEAADIIAKAIIERPRTIAPWWLWPAEVTTVLLRAPVERATQV
                     WWRRRARHSEAVAAEDR"
     gene            complement(1522..2922)
                     /locus_tag="VGP27_26765"
     CDS             complement(1522..2922)
                     /locus_tag="VGP27_26765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7854936.1"
                     /translation="MERLSGLDASFLYIESSTQPLHVCSILELDTSTVPGGYSFDRLR
                     DELASRIAAIPELRAKLADSQLNLDYPVWVDDDGFDLDRHLLRIKLPSPGGRRELVEI
                     CGHIASAPLDRDRPLWEMWVIEGVAETDPRAGGLLAVMTKVHHAAVDGVNGANLLSQL
                     CSAAPDMPPPEPVEGAGSAGPLQIAAGGLMRFAARPWQLVNLMPTTAATIVKTLRRAG
                     DGLTMAAPFTAPATRFNTSVTADRNIALVQLSLDDVKKVKDRFNVTVNDVVMALCASV
                     LRWFLSDYGELPAKPLVAMVPVSVHDKSDRPGHNQLSGMFCSLATHISDPAQRLRAIA
                     RSDAAAKNHSSAISPTLLQDWAQLAARAVFGAVFRLLADSPLIGNPVHNLIVSNVPGP
                     QATLYFLGCEVQAMYPLGPLFHGCGLNITVMSLDGKLNVGVVSCPDLLPDLWRLADDF
                     DVALQELLECDVPARR"
     gene            3061..>4087
                     /locus_tag="VGP27_26770"
     CDS             3061..>4087
                     /locus_tag="VGP27_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEV7854937.1"
                     /translation="MSLVDVVLLPIRVGRHIADAVMHAVVPQSPAPPSELFVVDGMPE
                     GVPPAARRPEPALPAPAGWPFGEEFPRTCGTSRFADGALFWTDFLYDDHGATGVPVSL
                     GKGGAPPRGTYVYPDGPAARNGADIFRVAIGLTDTDTWWRVDWNTLLDPSVPIALFTF
                     DTDRATTTTDEWPAKAGVRSAGIDLALLISASGARLIDLTTHASTPVEHTVDMAARSF
                     FARLPRALVEPAGTWTVRLAAGLANERGDGFADVPLRHGAKPGQPNVYNVAFRTHEQE
                     KAHLNFWSDQAQAAALSHGDVSQFSLAVPWEALADHTVTDEPVVRGVSTRWYVSSVEL
                     GQGVADTA"
BASE COUNT          706 a         1484 c         1272 g          625 t
ORIGIN      
        1 accggtcgtg ccggaggtca gcagaacgat tcggccggcc cgcgccacag caggccgcga
       61 accttcacgc atatcgacat ccgccgcatc gaccaccctg accccgtcgt ccgcgtcacg
      121 cacgcgcgaa gcgaactcgc tttcacagac gaccagtccg atccggtggc tgcgaagcgc
      181 ggcggccagc gattcggtcc gaaagtccgt gttgagcagc acgacgtcgg ctccgaccgt
      241 ggccgccgcg aaaacggccg cggcaaaagc ccgtccattg cggcacaaga ctccgaccgc
      301 gtgcccaggg ccgctgccgc ggcgttgcag ctgatcggcg agactgtcag tgcaccaaag
      361 caattcgcga tagctgatgg caccctcgtc gtcgatcatc gctgtccggt gcggccaccg
      421 agccgccgcc atccccagca aggtgaccag attcgcgcga cctcgcacca cttcgcgaac
      481 gaaccgcggc aatgccgccg gccccggcgg aatcagcagc ctggagcgca gcaatgcccg
      541 acaacccgtc gccaccaagc tctcagtcat cggtcctccg cggcgacggc ctccgaatgc
      601 cgagcgcgcc gccgccacca cacttgcgtc gcgcgctcca ccggcgcccg caggagcacc
      661 gtggtcactt cggccggcca caaccaccac ggcgcgattg ttcgaggccg ctcgatgatc
      721 gccttggcga tgatgtccgc cgcttcgtcg ggagtcaggc ccggcgcgtt gcgcaggctc
      781 tccgtcggcg cggtcatgcg ggtgtggatc agcgccatat agatcgacgt gacgtcgatg
      841 ccgtcggcgt gcaactccgc tgcgacgctg cgcagccaca tgtcgaacgc ccccttcgac
      901 gcctgataag cgccccaccg cggaccgggc gggacacgga caccgacgct cgagacattc
      961 acgatctgcc ccgtcccacg ctcccgcatc gccggcagca aacccagcag cagccagatc
     1021 ggaccgaggt agttgatgcc gatggtgcgg tggaaatcgt gcggccgctc gtattggtga
     1081 tgcagcgagc gccggatgga cttaccggcg ttgctcacca cgatatccgg cggcccgtgc
     1141 tcgtcggtga ttctttgcgc caacgcggtg gcggcggcct cgtcgctcag gtcggccggg
     1201 taactgaccg cgcgcccgcc gcccgcgtcg atcgcccgca ccagctcgtc gagcttgtcc
     1261 tccgaccggg ccgccagcag cacggtcgct ccggcggcgg cgagtgtgcg cgcggtggcc
     1321 tcaccgatac cgaacgacgc gccggtcacc agtacggtct tccccgacac cgcgtcgcgg
     1381 aggcggtctg gcgcagatcc atgcgatgga ttcacgactt tgtcgagggc tttcgtcgcc
     1441 aggctcacca gaacggactt ccccggagcc ggcgtgctca accccccgag cggctgagcc
     1501 gcgtgcgccg aagccaaggc ctcacctgcg cgccggaacg tcgcattcga gcaactcctg
     1561 aagcgcaaca tcgaagtcgt cggccaaccg ccacagatcg ggcaacaggt ccggacagga
     1621 gaccacgccc acgttcaact tgccgtccag cgacatcacg gtgatgttca ggccgcagcc
     1681 gtggaacagc ggtccgagcg gatacatcgc ctggacttca caacccaaga agtacagcgt
     1741 tgcctgcggc cccggcacat tcgaaacgat gaggttgtgc acggggttcc cgatcagcgg
     1801 cgagtcagcc agaagacgga agacggcgcc gaacaccgcc cgggccgcca gttgcgccca
     1861 atcctgcaag agcgtggggc tgatagccga gctgtggttc ttggcagcgg cgtccgaacg
     1921 ggcgattgcc cgtaatcgtt gcgcgggatc actgatgtgg gtggcgaggc tgcagaacat
     1981 ccccgacaac tggttgtgac ccggtcgatc ggatttgtcg tgcaccgaca ccggcaccat
     2041 cgccaccagt ggtttggccg gcagctcgcc gtagtcgctc aagaaccagc gcagcaccga
     2101 cgcgcacaaa gccatcacca cgtcgttgac cgtgacgttg aagcggtcct tgaccttctt
     2161 gacgtcgtcg aggctcaact gcaccaacgc gatgttgcgg tcggcggtga ccgatgtgtt
     2221 gaaccgggtt gccggtgctg tgaagggcgc cgccatggtc agtccgtcgc ccgcacgccg
     2281 cagggtcttc acgatcgtcg cggccgtcgt cggcatcaag ttcaccaact gccagggccg
     2341 cgcggcgaac ctcatcagcc cacccgccgc tatctgcagc ggaccggcac tgcccgcacc
     2401 ctccaccggt tccggcggcg gcatgtccgg cgcggcgctg cacagctgcg acaagaggtt
     2461 ggcgccgttc accccgtcca ccgcggcgtg atgaaccttc gtcatcaccg ccaacagtcc
     2521 gcccgcgcgc gggtcggttt cggcaacacc ctcgatcacc cacatctccc acagcggcct
     2581 gtcgcgatcc agcggtgcgg atgcgatgtg cccgcagatc tcgaccagct ctcggcgtcc
     2641 tcccggcgag ggcagcttga tgcggagcag atgacggtcg aggtcgaacc cgtcgtcgtc
     2701 gacccacacc ggatagtcca aattgagctg gctgtcggcc agctttgccc gcaactccgg
     2761 gatggccgcg attctcgagg ccagctcgtc gcgaaggcgg tcgaaactgt aaccccccgg
     2821 caccgtcgac gtgtccaact cgaggatcga acacacatgc aggggctggg tagaactttc
     2881 gatgtaaagg aaactcgcgt cgagcccact gagacgctcc atatgccgag gctaggcgca
     2941 atgaccacgc aacgaaggcg attgcgcaga acgccgacaa ttctcgaaaa ctccaccgtc
     3001 ggagcgcaat ccgtggccga agggttacat gcctggtccg ataccccata ggttgaaacc
     3061 atgtcattgg tagacgttgt cctcctgccc atccgcgtcg ggcgccacat cgccgacgca
     3121 gtgatgcatg cggtcgtacc gcagtcgccc gcgccgccgt cggagctgtt cgtcgtcgat
     3181 gggatgcccg aaggcgttcc gcctgcggcg cgccggcccg agcccgccct tccggcaccg
     3241 gccggatggc cgttcggcga ggagttcccg cgcacctgcg gcaccagccg cttcgccgac
     3301 ggtgccctgt tctggaccga cttcctctac gacgaccacg gcgcgaccgg cgtcccggtg
     3361 agcctgggta aaggcggggc gccgccacgc ggcacgtacg tctaccccga cggacccgcc
     3421 gcccgcaacg gcgccgacat cttccgagtc gccatcggcc tcaccgatac cgacacctgg
     3481 tggcgggtcg actggaacac gctgctcgac ccgtccgtcc ccatcgcact gttcacgttc
     3541 gacaccgacc gcgcaacgac aaccaccgac gaatggccgg cgaaagcggg cgtccgctcc
     3601 gccggcatcg acctcgcact gctcatctcg gcctccggtg cacggctgat cgacctcacg
     3661 actcatgcgt cgacaccggt ggaacacacc gtcgacatgg cggctcgctc cttcttcgcc
     3721 cggcttccgc gcgcattggt ggagccggca ggcacctgga cagttcggct ggccgcaggg
     3781 ctggcgaacg agcggggaga cgggttcgcc gacgttcccc tgcgccacgg cgcgaaaccc
     3841 gggcagccga acgtctacaa cgtcgcgttc cgcacccacg agcaggagaa ggcgcacctc
     3901 aacttctggt ccgaccaggc ccaggccgcc gccctctccc acggcgacgt gtcccagttc
     3961 tcgctggccg tcccatggga ggcgctcgcc gatcacaccg tcaccgacga gcccgtcgtc
     4021 aggggcgtct ccacccggtg gtacgtctcg tcggtcgagc tgggacaggg cgtcgccgac
     4081 accgcgc
//
LOCUS       DASYLX010001123         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_854207,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001123 DASYLX010000000
VERSION     DASYLX010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854207"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP27_26775"
     CDS             complement(<1..306)
                     /locus_tag="VGP27_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7854938.1"
                     /translation="MATRIVIIGGGPAGYEAALVAAARGRDVVEVTVVDSDGIGGACV
                     LWDCVPSKTLIASTGVRTELRRADGLGFDIAIEDAKISLAQIHSRVKTLARSQSADIG
                     "
     gene            373..855
                     /locus_tag="VGP27_26780"
     CDS             373..855
                     /locus_tag="VGP27_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEV7854939.1"
                     /translation="MPIYAAYGSNMHPDQMQERAPHSPMAGTGWLHGWRLTFGGEDLG
                     WEGALATLVEEPLSKVFVVLYDMTESDEQNLDRWEGSELGVHKKIRCRVDRLSSDTTT
                     DPVLAWLYVVDAWEGGLPSARYIGVMADAAEIAGAPAEYVRDLRNRPASNIGPGTVEA
                     "
     gene            complement(925..1812)
                     /locus_tag="VGP27_26785"
     CDS             complement(925..1812)
                     /locus_tag="VGP27_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854940.1"
                     /translation="MHADLERLLDDQQNVATSGQILAVISRRSFEAELKIGSLERIWQ
                     GIYCRGEPDDLLRLRGLDLSCDTRVAACLGTAAAIHGFDTEEPRELHVLSPQGRRLRS
                     ADGLVVHRRDGAPLVMVDERRVTSPAWTAVEVARSLRRPRALATLDAALRSATCSRAD
                     IWRAAIEQAGRRGIAAVRDLIPLADRRAESPMESEARLAMIDGGLPTPELQYEVVDGN
                     GELRRLDFAWPEHRVAVEYDGMDWHSGPDAMKRDRRRQAALWDIGWVVTAIVFDDVRY
                     RQWEFVGRIDAQLRRAQAA"
     gene            complement(2026..>2670)
                     /locus_tag="VGP27_26790"
     CDS             complement(2026..>2670)
                     /locus_tag="VGP27_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV7854941.1"
                     /translation="VRQGPITSAADHIEVTLHSQGGHTSRPHLTGDLVYGLGTLITGL
                     PGVLSRRIDPRTSTVMVWGAVNAGVAANAIPQTGILAGTVRTANRDTWLTLEAIVDET
                     ITALLAPLGIEHTLHYKRGVPPVVNEEISTRILTHAIETIGPDVLADTRQSGGGEDFS
                     WYLEDVPGAMARLGVWSGRGPQLDLHQPTFDIDERALAVGVRALVNIVEQAADF"
BASE COUNT          444 a          904 c          881 g          441 t
ORIGIN      
        1 gccgatgtcc gcggactgcg acctggccag cgtcttcacc cggctgtgga tctgcgcaag
       61 tgagattttc gcgtcctcga tcgcgatgtc gaagccgagg ccgtcggcgc ggcgcaactc
      121 ggtgcgcact cccgtcgacg cgatcagggt tttcgacggc acgcagtccc acagcacgca
      181 ggccccgccg atgccgtcgc tgtcgacgac ggtgacctcg acgacgtcgc gaccgcgggc
      241 cgcggcgacc agcgcggcct catagccggc gggaccaccg ccgatgatca cgatgcgggt
      301 agccaccgcc ttaacctagc taagcgtgcc gttgatcgcc cagccacgga acgaagcctt
      361 aggcttctcc ccgtgccgat ctacgccgct tacggttcga acatgcatcc ggaccagatg
      421 caggagcggg caccgcactc gccgatggcg gggacgggat ggctgcacgg ctggcggctc
      481 accttcggtg gtgaggacct cggctgggag ggcgcgctgg ccacgctcgt cgaggagccg
      541 ctgtcgaagg tgttcgtggt tctctacgac atgacggagt ccgacgagca gaacctcgac
      601 cgctgggaag gcagcgagtt gggggtgcac aagaagatcc ggtgtcgggt ggaccggctg
      661 tcgtcggaca ccaccacgga tccggtgctg gcctggctgt atgtggtgga cgcgtgggag
      721 ggcggcctac cgtcggcgcg gtatatcggt gtgatggccg acgcggccga aattgccggt
      781 gcgccagcag aatacgtccg cgatctgcgc aaccgcccgg caagcaacat cggccccggc
      841 acggtagagg catagccccc gtcggcccgc gagcgaccgt gtttgtacgc cgacacgccg
      901 tgtctggcgt catttcacgg tcgctcacgc cgcttgagcg cgacgcaatt gcgcgtcgat
      961 gcggccgacg aattcccact gccggtatct gacgtcgtcg aacacgattg cggtgaccac
     1021 ccagccgatg tcccataacg cggcctgacg cctgcggtcc cgcttcatcg catcgggccc
     1081 gctgtgccag tccataccgt cgtactcgac agcgacccga tgttcgggcc acgcgaaatc
     1141 caagcgcctg agttcgccgt tgccgtcgac tacctcgtac tgcagctccg gtgtcggcag
     1201 gccgccgtcg atcatcgcaa gccgagcctc gctctccatc ggcgactctg cgcgccgatc
     1261 ggccaacggg atgaggtccc tgaccgcggc gatgccgcga cggcccgcct gctcgatggc
     1321 ggcgcgccaa atatcggctc tgctacacgt cgcgctgcgc aacgccgcgt ccagcgtcgc
     1381 aagcgcccgc ggacgccgca gactgcgtgc gacctcgacg gctgtccatg ccggtgacgt
     1441 gacacggcgt tcgtcgacca ttaccagcgg cgcaccatcg cgacgatgaa ccaccagtcc
     1501 atcggcggat cgtaggcggc gaccctgcgg gctgagcaca tgcagttcgc ggggctcttc
     1561 ggtgtcgaag ccgtgaatcg cggcggcggt accgagacaa gcggccaccc tggtatcaca
     1621 tgacagatcg agtcctcgca gccgcagaag gtcgtccggc tcgccgcggc aataaatccc
     1681 ctgccaaatc ctctccaggg agccaatttt cagctctgct tcgaaactac gcctgctaat
     1741 gaccgcaagg atctggccgc tcgttgcgac attctgttga tcgtcaagaa gtcgttcgag
     1801 gtctgcatgc acagcgagat cgtcggcgcg ctacgggtgc gccggtagag gtcgacatcg
     1861 cttctgtgca tcaacggcgc tctgtggaca ctcatcgacg gcgagcgacc actaaatgac
     1921 caccaaacag ggcgcgtcga cgtacagaca cggtcgctcg cggtgcaacg ggtccctagc
     1981 gctcggggtg caacgggtcc ctagcgctgg cggtgcatcg ccttcctaga agtcggcggc
     2041 ctgctcaacg atgttcacca gcgcgcgaac gcccaccgcg agcgcccgct cgtcgatgtc
     2101 gaacgtcggc tggtgcaggt cgagctgcgg gccgcggccc gaccagacgc ccagccgcgc
     2161 catcgcaccg ggcacgtcct cgagatacca cgagaagtcc tcgccgccgc cggactgacg
     2221 agtgtcggcc agcacgtcgg gcccgatggt ctcgatcgca tgcgtcagaa tccgcgtcga
     2281 gatctcctcg ttgaccaccg gcggcacacc gcgcttgtag tgcagcgtgt gctcgatgcc
     2341 cagcggcgcc aacagcgcgg tgatggtctc gtcgacaatc gcttccaacg tcagccaggt
     2401 gtcgcgattg gcggtacgga ccgtgccggc gaggatgccg gtctgcggaa tggcgttggc
     2461 cgcaaccccg gcgttgacgg ctccccacac catcaccgtg ctggtgcgcg ggtcgatgcg
     2521 acgcgacagc acgccgggca gtccggtgat cagcgtgccg agcccgtaga ccaggtcgcc
     2581 ggtcagatgt gggcgcgagg tgtgtccgcc ctggctgtgc agggtgacct cgatgtggtc
     2641 ggccgcggac gtgatggggc cttgacgcac
//
LOCUS       DASYLX010001124         5656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1291326,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001124 DASYLX010000000
VERSION     DASYLX010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291326"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VGP27_26795"
     CDS             <1..1499
                     /locus_tag="VGP27_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7854942.1"
                     /translation="VMVLGYMQVPTLLGIGHLTVKLELPASGGLYRFSNVTYRGVQVG
                     KVTEVKPVGANHVEATLSLNNSPMIPSALTAQVRSVSAVGEQYVDLQPRGESGPYLQD
                     GSVIPMQNTAVPQQVGPMLDQLNALVSSIPQEKFGDLLDESFKAFDGAGYDLGSLLDS
                     SAKVTGDLNGVAARTRALIDDSAPLLDTQAQNTDAIRIWTRSLAGVTYQVGRNDPEIR
                     TLLRKGPGAADEVSKLLADVKPTLPLLLANLTTIGQIGVTYNPSIEQLLVLFPAYVSA
                     IQSVAPVNNPTGLALGDFSATLADPPACTVGFLPPSAWRSPDETSETDTPDGLYCKLP
                     QDSPVAVRGARNYPCMGHPGKRAPTVQLCDDPNGFVPLAQRQHALGPSPLDPNLVAQG
                     IPPDDRVDADAHLHAPIEGTPLPPGIPPAPEATPPGPPPPPEASTAPAAAPSSFAGNE
                     TGASPSVAFAQYNPRTGAYMSSDGHLYHQSNLVADKPDTWTDLVLASQ"
     gene            complement(1506..1973)
                     /locus_tag="VGP27_26800"
     CDS             complement(1506..1973)
                     /locus_tag="VGP27_26800"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7854943.1"
                     /translation="MSPVTYSREELAAAHDHLMARSGAAQGSGDWSDYCELFTDDAVY
                     VEHFLGTYHGRAEIKQWLVPAMTAWPQMTFPVLWRTFDEEGGVVVFAVGNVMPDVDGN
                     GPYSVPSWTLLEYAGENQWSREEDIYNPDAMLKMLSRWCEAAGVDFPAPDIHS"
     gene            2155..3198
                     /locus_tag="VGP27_26805"
     CDS             2155..3198
                     /locus_tag="VGP27_26805"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV7854944.1"
                     /translation="MTYKVVQWGTGNLGTLAVQSILKHPDLELIGTWVSSAEKEGRDV
                     GELSSVSPIGLLATQDADALIDGEADCICYAANSMGREDEVVADCVRMLGAGKNVVNV
                     SDPALVYPKAKGGDVYDRLQDACLEGNASFYTSGGDPGWAGFGLALAPLTVAQQVRSL
                     RLFEIYNYGQWNNPQLGLYGFGAPDTSASMILMPGVTASIWGPTVAMLADAMGVTLDS
                     IDEAHRVIHAEEDFDIPFGHIAKGTVSGMSFEIKGMVDGEERIVIEHITKLRRQDFPE
                     HGLEDVYRVVIEGEPNIKLDMWLTSDFGDTTHAGYIVAVTPVTNAIPTVCAAPAGVLT
                     YLDLPPHGARGLL"
     gene            complement(3221..4069)
                     /locus_tag="VGP27_26810"
     CDS             complement(3221..4069)
                     /locus_tag="VGP27_26810"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854945.1"
                     /translation="MRIVRAVTDFFGIDVGTQLAPLIATDKPPRLVTRRLEVQRTEFD
                     GMAVWTLLPPSPSGKVVIALHGGAYVVQPTVMHWSDYALMSRQTEATIVVPIYPLAPY
                     STAHEVVPRIADLITAQIERHAARTVSVYGDSAGGGLALAASQVLVRRRSATPARMVL
                     ISPWLDVSMTNPAIQGVDDPALDCESMQRSGRLWAGDLEPTDPLVSPVYGTLTGVPPT
                     AVYSGTLDLLNPDVLRLQEIASRTPGAEFTFVIRAGEMHDWAMGGLPEARAVRGRIYQ
                     QLLDTD"
     gene            4412..5206
                     /locus_tag="VGP27_26815"
     CDS             4412..5206
                     /locus_tag="VGP27_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854946.1"
                     /translation="MSEQPGAPRRFTYECDGLTLAYAVVGDGPQPIVFIHGATATGEF
                     EFGSLAAGLGPEYRCVLPDIRGHGRSEVADSIHKGEAVCTDLLRLIEHLELGRPHVVG
                     FSYGAEIALMLELSAPGTARSLVLISPGTGRPSDYRAPRLEHLYRTWPFALRRLHEAN
                     HGPEHWHHLVTALHEDSVGRAELSPQTLAGVECPTLLLAGEQDEATRRRQGQRFAELN
                     ERARYVEIPGAPHAAHHKSPDTVKRVIGDFLAEVDLEKAGNYGAVN"
     gene            5190..>5656
                     /locus_tag="VGP27_26820"
     CDS             5190..>5656
                     /locus_tag="VGP27_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854947.1"
                     /translation="MARSTEVKPTKSQPPAEVHVPSWQRDSVDRSLRNARARAQARSD
                     RFVAAAIELLGERDESDFTIQDVVDKSTMSQRTFYTFFDGKDSLLLAVYETILRTTAM
                     PIFRERCEGIKDPVKRLRALLEALSEITAMPARLSRGLSVLHLRLSESRPNDLA"
BASE COUNT         1019 a         1795 c         1842 g         1000 t
ORIGIN      
        1 tggtgatggt gctgggctat atgcaggtac cgaccctgct tggcatcggg cacctcaccg
       61 tcaaactcga gctgccggcc tccggtggtc tctaccggtt cagcaacgtc acctatcgcg
      121 gcgtgcaggt cggaaaggtg accgaggtca agcccgtggg tgcgaaccac gtggaagcga
      181 ctctgtcgct gaacaattcg ccgatgatcc cgtcggctct caccgcgcag gtccgcagcg
      241 tgtcggcggt cggcgaacag tacgtcgacc tccagccgcg cggtgagtct ggcccgtatc
      301 tgcaggacgg gtcggttatt ccaatgcaga acaccgcggt tccccaacag gtggggccca
      361 tgctcgatca gctcaacgcg ctggtgagca gcattccgca ggagaagttc ggtgacctgc
      421 tggacgagtc gttcaaggct ttcgatggtg caggctatga cctgggttcg ttgctcgact
      481 cgtcggcgaa ggtcaccggc gacctcaacg gggtcgccgc ccggacacgt gccctcattg
      541 acgacagcgc acctctgctg gatacgcagg cgcagaacac cgatgcgatc cgaatctgga
      601 cacgcagcct ggcaggcgtc acctatcagg tggggcgcaa cgatccggag atacgcacac
      661 tgctgcgaaa ggggccgggc gccgccgacg aagtgtccaa gctgctcgcc gacgtcaagc
      721 ccacgttgcc gctgttgctg gccaacctca ccacaatcgg ccagatcggt gtgacgtaca
      781 acccgtcgat cgagcagttg ctggtgttgt tcccggccta cgtctcggcg attcagtcgg
      841 tggcgcccgt caacaatccc accggtctgg cattgggcga cttctcggca accctcgccg
      901 acccgcccgc gtgcacggtc ggattcttgc cgccgtcggc gtggcggtcg ccggacgaga
      961 cctccgaaac cgacacgccc gacggtctgt attgcaaact gccgcaagac tctccggtcg
     1021 cggtgcgggg tgcgcggaac tatccgtgca tgggccatcc gggaaaacgg gcgccgacgg
     1081 tgcaactctg tgacgatccg aacggattcg taccgctggc gcagcgccag cacgcgctgg
     1141 gcccgtctcc gttagacccc aacctggtcg ctcagggcat cccgccagac gatcgggtcg
     1201 atgccgacgc gcatctccac gctccgatcg aaggcacgcc gttgcccccg ggtattccac
     1261 ctgcaccaga ggcgactccg ccggggcccc cgccgccacc ggaagcctcc accgctcccg
     1321 cagcggctcc aagttcgttc gccggcaacg aaactggcgc gtctccgtcg gttgcgttcg
     1381 ctcagtacaa tccgcgaacg ggtgcgtaca tgtcgtcaga cggccatctg tatcaccagt
     1441 cgaatctcgt tgccgacaag cccgatacgt ggacagacct ggttttggcc tcgcaatgag
     1501 ggcagtcagc tgtgaatgtc gggggcaggg aagtcgacgc ccgccgcctc gcaccaacga
     1561 ctcagcattt tgagcatggc gtcggggttg tagatgtctt cctcacgcga ccactggttc
     1621 tcgcccgcat actcgagcag agtccacgac ggcaccgaat agggtccgtt cccgtcgaca
     1681 tcgggcatga cgtttccgac ggcgaacaca acgaccccgc cttcctcgtc gaacgtgcgc
     1741 cacaacacgg ggaaggtcat ttgcggccac gcggtcatgg ccgggacgag ccactgcttg
     1801 atctcggcgc ggccgtgata ggtgcccaag aagtgctcga cgtagacggc gtcgtcggtg
     1861 aacagctcgc agtagtcgga ccagtcgccg ctgccctggg cggcgccgct gcgggccatg
     1921 agatggtcgt gggccgccgc caattcttcg cgcgaatacg tcactggact cacttctctc
     1981 tcgttatgtg ctcgaccaga gtggctgtag tggaaatcga catctcatcg acgaacagca
     2041 ggggttaccg atctgatacg gctcactgtc ttaaaacgaa cactacattc tactttttgt
     2101 agaaggatac attcagttag accgccactg cccaacagga tcggagtaac aaccatgacc
     2161 tacaaggtcg tccaatgggg gacggggaac ctgggcaccc tggccgtgca gagcatcttg
     2221 aagcatccgg atctcgagct catcggaacg tgggtgtctt cggcggagaa ggaggggcgc
     2281 gacgtcggag agctgtcgag tgtgtccccg atcggcctgc tggcaaccca ggatgccgac
     2341 gcgctgatcg acggcgaggc cgactgcatc tgttatgccg ccaacagcat gggccgcgag
     2401 gatgaggtgg tcgccgactg cgtacgcatg ctcggggccg gcaagaacgt cgtcaacgtg
     2461 tccgatccgg cgctcgtcta ccccaaggcg aagggcggtg acgtctacga ccgtctgcaa
     2521 gacgcctgcc ttgagggcaa cgcgtcgttc tacaccagcg gcggggaccc gggctgggca
     2581 ggattcgggt tggcgttggc gccgctcacc gtcgcccagc aggtccgttc gctacggctg
     2641 ttcgagatct acaactacgg ccagtggaac aatccgcagc tgggcctcta cggtttcggc
     2701 gcacccgaca cctcggcctc gatgatcctg atgcccggcg tgacggcgag tatctggggg
     2761 cccacggtcg ccatgctggc cgacgcgatg ggcgtcacgc tcgacagcat cgacgaggcg
     2821 caccgcgtca tccacgccga agaagacttc gacatcccgt tcggccacat tgcgaagggg
     2881 accgtctcgg gcatgtcgtt cgagatcaag ggcatggtgg acggcgagga gcgcatcgtg
     2941 atcgagcaca tcaccaaact gcgtcgacag gacttccccg agcacggact cgaggacgtc
     3001 taccgcgtgg ttatcgaggg cgagcccaac atcaagctgg acatgtggct cacctcggat
     3061 ttcggcgaca ccacccacgc gggctacatc gtcgcggtca cgccggtgac caatgccatt
     3121 ccgacggtct gcgccgcgcc tgcgggggta ctcacctacc tcgacctgcc gccacatggc
     3181 gcccgcggcc tgctctgagc ccggagtcag cggcttcgtg ctaatcggtg tcaagcagct
     3241 gctggtagat ccggccgcga acggcccgcg cttcgggcag gccgcccatc gcccagtcat
     3301 gcatttcgcc tgcgcggatc acgaaggtga attccgcgcc gggggtgcgg gacgcgatct
     3361 cctgcagtct cagcacatcc gggttgagta ggtcgagggt tccgctgtaa acggcggtcg
     3421 gcggaacgcc ggtcagcgtg ccgtagacgg ggctgacgag gggatcggtg ggttcgaggt
     3481 caccggccca tagtcgccct gaacgctgca tggattcgca gtcgagagcg gggtcgtcga
     3541 caccttgaat ggcagggttg gtcatggaga cgtccagcca cggtgagatg agcaccatgc
     3601 gcgccggcgt ggccgatcgg cggcgcacca ggacttggga tgcggcgagg gccagaccgc
     3661 cgccggcgga gtccccgtac acgctgacgg tgcgagcggc gtgacgctcg atctgtgcgg
     3721 tgatcaggtc ggcgatgcgt ggcacgacct cgtgggctgt tgaatatggg gccagcggat
     3781 agatcggcac gacaatggtc gcttcggttt ggcgcgacat caatgcgtag tcagaccagt
     3841 gcatgacggt gggttgcacg acgtacgcgc ctccgtgcaa ggcgatcacg accttgccgg
     3901 agggcgaggg cggcaacagt gtccacaccg ccatgccatc gaactcggtg cgctgcacct
     3961 ctagtcgccg ggtcaccagc ctgggcggtt tgtcggtggc tatcagcggc gccaactgcg
     4021 tgcccacgtc gatgccgaag aagtcggtga cggcgcggac aatccgcaac ccgcgaatcg
     4081 ttgcccgcgc gatggaagac ggcccctggg cccccgtggt gggcttcgta ttggcgccgt
     4141 cggcgtgaag gggatcgcta ctaatgggag agcaactcct ttccggtgcg ccgatggtat
     4201 tgcagatggg gcccgcgaga gcagtggctg ttgtctgaga agcacctccg cttatcttct
     4261 tggtagaatg ccatcagcac caggcgacag tcgcagggat tccaactgga aggcttcagg
     4321 gtgaacttgg ccgatctggc gtcgtcgtca gtcgtcagac gacgcgaatc gctgccgaat
     4381 cccgagaatc atgatgtcgt tgaaggaagt catgtctgag cagccaggtg caccgcgacg
     4441 tttcacttac gaatgcgacg ggttgacgct ggcgtatgcc gttgtcggtg acgggccaca
     4501 gccgatcgtg ttcatccacg gcgccaccgc caccggtgag ttcgagttcg gctcgctggc
     4561 cgccgggctg ggacctgagt atcgctgtgt gctacccgac atccgcggcc acggtcgatc
     4621 tgaggtcgcg gattcaattc ataagggcga ggcggtgtgt accgatctgc ttcgcctcat
     4681 cgagcatctg gaactcggtc gacctcacgt cgtgggtttc tcgtacggcg cggagatcgc
     4741 gctgatgctt gagctttcgg ctccgggaac agcgcggtcg ctggtgctga tcagcccagg
     4801 gacgggccgg ccgagcgatt accgggcgcc gcgtctggag cacttgtacc gcacctggcc
     4861 gttcgcgctg cgacgcctcc acgaggcgaa ccatggcccg gaacactggc accatctggt
     4921 cactgccttg cacgaagaca gcgtcggacg ggccgaactg tcgccgcaga cgctcgccgg
     4981 cgtcgagtgc ccgactctgc tactggcggg cgaacaggac gaagcgacgc gccgccggca
     5041 gggacagcgg ttcgccgaat tgaacgagcg ggcacggtat gtcgagatcc cgggcgcgcc
     5101 ccacgccgcc caccacaaga gcccggatac cgtgaagcgg gtcatcggtg acttcttggc
     5161 tgaagtcgac cttgagaaag cggggaacta tggcgcggtc aactgaggtg aagccgacga
     5221 agagccagcc gccagccgag gtgcacgtac cgagttggca gcgcgacagc gtcgaccgct
     5281 cgctacgcaa cgcccgcgcg cgtgctcagg cccgaagtga tcgtttcgtc gcagcggcga
     5341 tcgagctgct cggggagcgc gacgaaagcg atttcaccat ccaggacgtg gtcgacaaat
     5401 ccacgatgtc ccagcgcacc ttctatacct tcttcgacgg gaaagacagc ctgctgttgg
     5461 ccgtctacga gacgattctg cgcaccaccg cgatgccgat attccgggag cggtgcgagg
     5521 gcatcaagga tccggtcaag cgattacggg cccttctgga ggcgctgtcc gagatcaccg
     5581 ccatgcctgc ccggctttcg cgcgggctga gcgtccttca tctgcggctc agcgagtccc
     5641 gccccaatga cctcgc
//
LOCUS       DASYLX010001125         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1624105,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001125 DASYLX010000000
VERSION     DASYLX010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624105"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..853
                     /locus_tag="VGP27_26825"
                     /pseudo
     CDS             1..853
                     /locus_tag="VGP27_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242540.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
     gene            912..1391
                     /locus_tag="VGP27_26830"
     CDS             912..1391
                     /locus_tag="VGP27_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV7854948.1"
                     /translation="MAQRVWLTATQQRTWLNYMGVYHRLEYEMNRQLQADVGISLADY
                     TVMNALSNEPGRRLQSSRLATRIGWERSRLSHQLRRMETRRLVERIASEVDGRATDAK
                     LTPQGWRLLQSAAPKHVAWVRTLFFSDLDDAAAGELADLLGEVYETILRNGTLPPPD"
     gene            complement(1406..2152)
                     /locus_tag="VGP27_26835"
     CDS             complement(1406..2152)
                     /locus_tag="VGP27_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7854949.1"
                     /translation="MGVVQGKVALITGGTSGIGLAAASRLAAEGAHVFITGRSRQRLD
                     DAVAQIGHGATGVVSDVSDFADIDAVAEAIQAHGHGLDVVFTNAGGGEFATIADVTVE
                     NYLDTFNRNVGGTLFTVQKVLPLLNRGASVILAGSTAASKGTPAFGVYAASKAAVRSL
                     GRTMAVELADRGIRVNTLVPGPVETPGLVGLAPAGGESALLAQMADGVVMKRVADPSE
                     IAATVAFLASDASSFMTGSELFVDGGEAQV"
     gene            complement(2262..>2414)
                     /locus_tag="VGP27_26840"
     CDS             complement(2262..>2414)
                     /locus_tag="VGP27_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEV7854950.1"
                     /translation="INFFGWNWNLPLGVAILLSAVGGGLLTVAVGTARIVQLRRAAKK
                     NLKRRL"
BASE COUNT          419 a          809 c          787 g          399 t
ORIGIN      
        1 cggcctgacg ccgtcggcac acgatatggc gcgcgaatac cgggtcgtcg ccgcgttgca
       61 ggacaccctc gtcccggtcg cgcgggctgt caccatgcgc aacgacgact ccgtcggcgg
      121 cgcgccgttc cagatggtcg aatacgtgcc cggcttggtc gtccggcaca ccggtgaact
      181 cgaggcgctg ggtgacaagg cggcgatcag cagctgcatc gacgcgttga tcaaggtgct
      241 cgccgacctg cacgccgtcg atccggcggc cgtcggcctc ggcgacttcg gcaaacccac
      301 cggctatctg gagcgccaag tgcggcggtg gggctcgcag tgggatctgg tcaagcgtga
      361 gaacgacccc ttcgacgccg atgtccgacg gctgcattcc gctctcgcgg aggctcttcc
      421 gtcgcagagc cgcagctcga tcgtgcacgg tgactatcgc atcgacaaca cgatgctgga
      481 catcgacgac gccaccaagg tgcttgcggt gctggactgg gagatgtcca cgctgggcga
      541 cccgatcagc gacgccgcgc tgatgtgcgt gtaccgccac cctctgttca acgtgatcca
      601 cgcgagctcg gcgtgggctt cgccgctcat gcccgaggcc gacgacctcg cgaaccgcta
      661 gtccgttgcg gccgaacagt cactggacaa ctgggacttc tacatggcgc tcggatattt
      721 caaggtggcg atcatcgccg cgggcatcgc gttccgggcg aggatgggcg ggggcgtcgc
      781 ggccgacacc gatcaagtgg accaggccgt cgggccgctg atggccgaag gcctcaacgc
      841 actcggccga tgacggccga gcagccgctg aagattgcgt gatacgtcaa ccacctgtga
      901 tgatgaacgc catggcgcag cgcgtatggc tcaccgcgac gcagcagcga acgtggctca
      961 actacatggg ggtctatcac cggctcgaat acgagatgaa ccggcaactt caagccgacg
     1021 tcggcatttc gctcgccgac tacaccgtca tgaacgcact gtcgaacgaa ccggggcgca
     1081 ggttgcagtc gagcaggctg gccacccgaa tcggttggga gcgaagcagg ctgtcgcacc
     1141 agcttcgtcg catggagacg cggcggctcg tcgagcggat cgcctcggag gtcgacggcc
     1201 gcgccaccga cgccaagctg acgccgcaag gctggcgcct gctgcagtcc gccgcgccca
     1261 agcacgtcgc ctgggtgcgc acgttgttct tctccgactt ggacgacgcc gcagccggcg
     1321 agctcgccga cctgctcggt gaggtttacg agacgatcct gcggaacggc acgttgccac
     1381 cgcccgactg acccgagtac gggggtcaga cttgcgcttc gccgccgtcg acgaacagtt
     1441 cactgcccgt catgaagctg ctggcgtcgg aggccaggaa ggcgacggtc gcggcgatct
     1501 cgctcggatc ggccaccctc ttcatgacga ccccgtcggc catctgcgcc aacaacgcgg
     1561 attcgccgcc tgcgggtgcc agcccgacca acccgggcgt ctcgacggga cctgggacca
     1621 gtgtgttgac ccgaatgccg cgatcggcca gttcgacggc catggtgcgg ccgagggatc
     1681 gaaccgccgc cttcgacgct gcgtagacac cgaacgcggg cgtgcccttc gacgccgccg
     1741 tcgagccggc caggatgacc gacgcgccgc ggttgagcaa cgggagaacc ttctgcactg
     1801 tgaagagggt gccgccgacg ttgcggttga aggtgtcgag gtagttctcg acggtgacgt
     1861 ccgcaatcgt cgcgaattcc ccaccgcctg cgttcgtgaa aaccacatct agtccgtggc
     1921 cgtgggcttg gatcgcttcg gcgaccgcgt cgatgtcggc gaagtcgctc acgtcgctca
     1981 ccacacccgt tgcgccatgg ccgatctggg cgaccgcgtc gtcgagccgt tgtcggctgc
     2041 gcccggtgat gaagacgtgc gcaccctcgg cggcgagccg gctcgcggcc gccaagccga
     2101 tgcccgacgt gccgccggtg atcaatgcaa ccttgccctg taccacaccc acgatgcccg
     2161 cctccgatgc attacgtgat caatcacgtt aatctgtgta cgtgacctgt caacaaaacc
     2221 acgtcggatc tattccagct gtgtgacgag gtaagccgcc gctagaggcg gcgcttcaga
     2281 ttcttcttcg ccgctcggcg gagctgaacg atgcgggcgg tgcccaccgc aacggtcagg
     2341 aggccaccgc ccacggctga cagcaggatc gcgacgccta gtggcaggtt ccagttccat
     2401 ccgaagaaat tgat
//
LOCUS       DASYLX010001126         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_746457,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001126 DASYLX010000000
VERSION     DASYLX010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746457"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1921)
                     /locus_tag="VGP27_26845"
     CDS             complement(<1..>1921)
                     /locus_tag="VGP27_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV7854951.1"
                     /translation="DISGLSADEKRALLSRLLRERAESSASTHPLSFGQRSLWFLYNL
                     APGSPAYTITYAGLISGDLDVPALERAAQALIDRNAVLRTTYGVRDGQPVQLVHPHWR
                     VRMARHDLGPDEHELDDWLRRESNHPFDLEIGPVLRLTLLRRGPHEHVLVLVMHHIAV
                     DFWSIDVMLDELRLLYAAQFGGQTPPVCPQRYVDHVDRQNQLLEGEHGERLWEYWREQ
                     LSGELPIMQLPTDRPRRVAQTYRGAVHRFTLDAGLTEDLKKTGRSASSTPYMTLLAAY
                     ATLLHRYNGQEDLLIGSPFANREYSGLEELIGYLANPLVLRADLHGDPTFSELLGRIK
                     ETVLGALRHQEYPFASLVERLRPVRDASHAPLFQVSFAWEQFRRFHDGADQGADGTAL
                     DLTSVHIGQGGAPDDLMMLVGELDGKLICALQYNTDLFDDSTIERMAGHFTTLLRGIV
                     TDRDKRLSELPFLTADETRRQAEWNETRVHFDAPDCLHEIIAETARRNPAAVAVTYED
                     RDMTYAELDRRADALAHRLQSMGVRTDTIVPVVLDRSEDLVVAMLAALKAGGAFMPID
                     PTQPTNRIATTVANAPGVPVCITHQRHIDRIEGFTGELLCLDALSVPLQQDAVARPTA
                     TTSAHTAYVIHTSGST"
BASE COUNT          328 a          593 c          674 g          326 t
ORIGIN      
        1 cggtggaccc ggatgtgtga atgacgtacg cggtgtgtgc cgacgtcgtg gcggtcggcc
       61 gcgccaccgc gtcttgctgc agagggaccg acagcgcgtc gaggcacaag agttcgccgg
      121 tgaatccttc gatgcgatcg atatggcgct ggtgcgtgat gcacaccggc acgcccggtg
      181 cattggccac agtggtggca atgcgattgg tgggttgtgt cgggtcgatc ggcatgaagg
      241 cgccaccggc cttgagagcg gccagcatcg cgacgacgag gtcctccgag cggtcgagga
      301 ccacgggcac gatcgtgtcg gtacgcacgc ccatgctctg caaccggtgg gccagcgcgt
      361 ctgcgcgccg gtcgagttcc gcgtaggtca tgtcgcggtc ctcgtaggtc acggcgaccg
      421 cagcggggtt gcgtcgggcc gtctccgcga tgatctcgtg cagacaatcg ggagcgtcga
      481 aatggacccg ggtctcattc cactccgcct gtcggcgagt ctcgtcggcg gtcaagaacg
      541 gcaactccga caaccgcttg tcgcgatcgg tgacgattcc gcggagcagc gtggtgaagt
      601 ggcccgccat gcgttcgatc gtcgagtcgt cgaagagatc ggtgttgtac tgcagagcgc
      661 agatcaactt tccgtccaac tcgccgacca gcatcatcag gtcatcgggt gcgccgccct
      721 ggccgatgtg aacggacgtc aggtcaagtg ccgtgccgtc agccccctgg tccgcgccgt
      781 cgtggaagcg ccggaactgc tcccaggcga aggagacctg gaacagcggc gcgtggctcg
      841 cgtcccggac tggccgtaac cgctcgacca acgaagcaaa tgggtactcc tggtgccgca
      901 gggcgccgag aaccgtctcc ttgatgcggc cgaggagttc tgagaacgtc gggtcgccgt
      961 gcagatcggc gcgcagaacc agcggattgg caaggtagcc aatgagttcc tcgagccctg
     1021 aatattcgcg gttggcgaac ggcgatccga tgagcaggtc ctcctgcccg ttgtagcggt
     1081 gcaggagggt ggcgtatgcc gccaacaggg tcatgtacgg cgtcgagctc gcgctgcggc
     1141 cggttttctt caagtcctcg gtgagcccgg cgtcgagggt gaagcggtgc acggcgccgc
     1201 gatatgtctg agccacccgc cggggccggt ccgtcggcaa ctgcataatc ggcaactcgc
     1261 cggatagctg ctcgcgccag tattcccaga gacgttctcc gtgctcgccc tcgagcagct
     1321 ggttctgccg gtcgacatgg tcgacgtagc gttgggggca caccggtggc gtctgcccgc
     1381 cgaattgagc ggcgtacagc aggcgcagct cgtcgagcat gacgtcgatg gaccagaagt
     1441 cgacggcgat gtgatgcatc acgaggacca gcacgtgctc gtgcggtcca cgccgtagca
     1501 gcgtcagccg cagaaccggg ccgatctcga ggtcgaacgg atggttcgac tcccggcgca
     1561 gccagtcgtc gagctcatgt tcgtcgggac cgaggtcgtg ccgggccatc cgcacccgcc
     1621 agtggggatg taccaactga accggctggc cgtcacgcac accgtaagtg gtgcgcagca
     1681 cggcattccg gtcgatgagc gcctgggcgg cccgctccag cgcggggacg tccaggtcac
     1741 cgctgatcaa gcccgcgtag gtgatggtgt aggcggggct gccgggagcg aggttgtaaa
     1801 ggaaccacag cgaccgctgt ccgaacgaca gcggatgcgt cgaggccgac gactccgcgc
     1861 gttcacggag gagccgcgac aacagcgcac gcttctcatc ggccgagagg ccggatatgt
     1921 c
//
LOCUS       DASYLX010001127         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1732134,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001127 DASYLX010000000
VERSION     DASYLX010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732134"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGP27_26850"
     CDS             complement(<1..304)
                     /locus_tag="VGP27_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7854952.1"
                     /translation="MQIGFIGLGNMGAAMAANLVKAGHDVTVYNRSRAKVDPLVAEGA
                     RPADSVADACRGDVVITMLANDDAVEAVTFGDGGILASLGRGATHVSSSTISVPLSE"
     gene            417..896
                     /locus_tag="VGP27_26855"
     CDS             417..896
                     /locus_tag="VGP27_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV7854953.1"
                     /translation="MKFSTKFSTKFSTKFSTKFSTTMLQDGNNTGIEVPADVVDALGS
                     GRRPAVVVNVNGFEYRSTVASMGGRYLLPFSAARRQESGIGGGDQIDVALTLDTAPRT
                     VEVPADLRAALDASPSAAAAWETLSTSNRRAQVESVLGAKAAQTRARRIAAIVAKLG"
     gene            953..1189
                     /locus_tag="VGP27_26860"
     CDS             953..1189
                     /locus_tag="VGP27_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854954.1"
                     /translation="MSSSPDGGDRVPIENVRCGDPIYDADGGCQHYKVLESRAIAGGG
                     VVLELECLHNNQCQVIEKSFPAGYLVARSTRHFL"
     gene            complement(1199..1585)
                     /locus_tag="VGP27_26865"
     CDS             complement(1199..1585)
                     /locus_tag="VGP27_26865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854955.1"
                     /translation="MTEHGPQTRNVLEYQELMKQLVPTVEAPEDWAPLESLVAVDDFE
                     RVGTRREVQDWQQYVHLLTGWASAIDRFETTVHRVSELPDLVYFAAEERHFRDQEITV
                     VNSLSVFEFDAAAKIRRLSVYLQQAR"
     gene            1621..2205
                     /locus_tag="VGP27_26870"
     CDS             1621..2205
                     /locus_tag="VGP27_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854956.1"
                     /translation="MGSAPPQVTDDLGTTEYSAARTRVLDAALELFADHGVSGTSLQM
                     IADAIGITKAAVYHQFRTKGDIVIAVTERELGRLVPALEEAEAHSQGSQARDALLIGV
                     INMAVRDRRLVRTLQFDPIVVRLLAEHEPFQRFMSRLYRVLLDEAGEDNLIQVAMLSG
                     AISSAVMHPLVGDIDDETLRSKLTDFSRRLMDLR"
     gene            2286..3089
                     /locus_tag="VGP27_26875"
     CDS             2286..3089
                     /locus_tag="VGP27_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854957.1"
                     /translation="MLTWLAPIIVVVVVALAVTAKLLVRRRAPEGGWFTDFPRSAGSL
                     SVISTMFAVMLAFVILFSLQSFQRAREGASIEAVSVTELSAVASLFPAPTGDRLHGGL
                     VCYGRAVVNDEWPAMEHGRSSALVESWVDTLQADFTAAAPQESQHEAAYAQWFDEVAQ
                     RREGRRERLAEASPFLPMPLWFALGVGATLTLAYMVVQADKREKMVIQAIPIGFVAAL
                     ITAGMLVVVFLDNPYTGGNGSIKPAEMNRTLMRIDNGGQVPCDQRGNPT"
     gene            complement(3082..3882)
                     /locus_tag="VGP27_26880"
     CDS             complement(3082..3882)
                     /locus_tag="VGP27_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEV7854958.1"
                     /translation="MKPRLRSRTNKGGGGFKLIVVSRLFAATGVVAATVFAPFLTAPA
                     ASAAPCPDVELVFARGTFEPPGVGGTGQAFVDSLRPKLGDKSLDVYPVEYPASLDFQT
                     AADGVIDASNKVQAVAAECPKTKIVLSGYSQGAAVTGYLTADSVPAGFQMPAGLTGPM
                     PPAIADHVAAVALFGKPSSGFLQMIANTAPPITVGHLYTAKTVDLCIPEDPICSPGGG
                     DSNAHNMYPANGLTDQAAEFVAQKVSAAPSTPSTSSPSTSAPSVQTAR"
     gene            4165..>4526
                     /locus_tag="VGP27_26885"
     CDS             4165..>4526
                     /locus_tag="VGP27_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HEV7854959.1"
                     /translation="MVNFSAPARRLLPAAVLALAVVGAPAVAVMAMPGAVIATPLAAC
                     TSGEEEDVYTTSCVPFMVPNSPDDSGFTTNAANPDIPEIDGVPCTGHDSGACIGLSEE
                     QQDMGPQPVPRSTISSSP"
BASE COUNT          769 a         1459 c         1502 g          796 t
ORIGIN      
        1 gttcggagag cggcacgctg atggtcgacg acgacacgtg agtcgcaccg cggccaagcg
       61 aggcgaggat tccgccgtcc ccgaaggtga ccgcctcgac ggcgtcgtcg ttcgcgagca
      121 tggtgatcac gacgtcaccg cggcacgcgt cggcgaccga gtccgccggc cgtgcgccct
      181 cggcgacgag ggggtccacc ttggcccgcg accggttgta gacagtgacg tcatggcccg
      241 ccttgacgag gttcgcggcc atggccgctc ccatgttgcc cagtccgatg aagccgatct
      301 gcagtttgtc cctggccatg cgccgagtat gcgcccgtca cccggctctc gaatcgactc
      361 gagccaaatc cgcagggctc agcgaatttt cgtcagttct gtgctatgcc ggatgcgtga
      421 agttctcgac caagttctcg accaagttct cgaccaagtt ctcgaccaag ttctcgacaa
      481 cgatgctcca ggacggcaac aacaccggca tcgaagtccc ggccgacgtg gtggacgctc
      541 tcgggtcggg taggcggccg gccgtggtgg tgaatgtgaa cggcttcgag taccgctcga
      601 cggttgcctc catgggcggc aggtatctgc ttccgttcag cgcggccagg cggcaggagt
      661 cggggatcgg gggaggtgac cagatcgacg tcgcgttgac gctcgacacc gcgccgcgga
      721 ccgtcgaggt gcccgcggac ctacgtgcgg cgctggacgc ttcgccttcc gcggcggccg
      781 cctgggagac gctctcgacc agcaaccgca gagcgcaggt cgaatcggtg ctgggcgcca
      841 aggccgccca aacccgcgcc cggcgcatcg cagccatcgt cgccaagctc gggtaggccc
      901 attgcgtgcg aaatgggtca tggcgccctg gccgcggctt accctcgtcg atatgtcttc
      961 atctcccgac ggcggggacc gagttccgat cgagaacgtc cgttgtggtg accccatcta
     1021 cgacgcggat ggcggttgcc agcactacaa ggtcttggag agcagagcca tcgcaggcgg
     1081 cggcgtcgtt ctcgagctgg agtgcctgca caacaaccag tgccaggtga tcgagaagtc
     1141 attcccggcg ggttacctcg tcgccagatc cacccgccac ttcctctagg gctcggatct
     1201 accgcgcctg ttgcaggtag acggacaggc ggcggatctt cgcggccgcg tcgaactcga
     1261 acacgctcag cgagttcacg acggtgattt cttgatcacg gaagtgccgc tcctcggccg
     1321 cgaagtacac caggtcaggc aattcggaga cgcgatgcac ggtggtctcg aacctgtcga
     1381 tcgcgctggc ccagcccgtc aacaggtgga cgtattgctg ccagtcctga acttcgcggc
     1441 gcgtgccgac tcgctcgaag tcgtcgacgg ccaccagtga ctccagcggg gcccagtcct
     1501 ccggcgcttc caccgtaggc accagttgct tcatgagctc ttggtactcc agaacattcc
     1561 gcgtctgcgg tccgtgttca gtcatgcgcc cgaccgtagc cggtcggcta gtctcaacgg
     1621 gtgggatcag cgccaccgca ggtaaccgat gacttgggga ccaccgagta cagcgcggca
     1681 cgcacccgcg tgcttgacgc cgcgttggag ctgttcgccg accacggggt tagcgggacg
     1741 tcgctgcaga tgatcgccga cgcaatcggc atcaccaaag cggcggtgta tcaccagttc
     1801 cggaccaagg gcgacatcgt catcgccgtg acggagcgcg aactgggcag gctggtgcct
     1861 gcgctcgaag aggccgaggc gcacagtcaa gggtcacagg cgcgcgacgc tctactgatc
     1921 ggcgtgatca acatggcggt gcgcgaccgc aggctggtgc ggaccctgca gttcgatccc
     1981 atcgtggtgc ggttgctcgc cgaacatgaa ccgttccagc gcttcatgtc gcggctgtac
     2041 cgggtgttgc tcgacgaggc cggcgaggac aacctcatcc aggtggcgat gttgtcgggc
     2101 gcgatcagca gcgccgtcat gcatccgctc gtcggcgaca tcgacgatga aaccctcaga
     2161 tcgaagctca ccgatttcag ccgccgcctg atggaccttc gctgagcgac cttcgcttgc
     2221 gccgaggccg gacgttcggt gaattgccgg ccgagtcgga cgcgcggcca atagagtaac
     2281 tgtccgtgct gacctggctc gccccgatca tcgttgtggt cgtagttgcg ttggctgtca
     2341 ccgcgaagct cctcgtccgc cgacgcgcgc cggaaggtgg ctggttcacc gacttcccgc
     2401 gcagcgcggg gtcgttgtcg gtgatcagca cgatgttcgc ggtgatgctc gcgttcgtga
     2461 tcctcttctc gctgcagagt ttccagcgcg cgcgagaggg cgccagcata gaagccgttt
     2521 ccgtgaccga actcagtgct gtcgcaagcc ttttcccggc gccgaccggt gatcgcctgc
     2581 atgggggctt ggtctgctac ggacgcgctg tcgtcaacga cgagtggccg gccatggagc
     2641 acggccggtc cagcgcactg gtggagtcgt gggtcgacac gctacaagcc gacttcactg
     2701 cggccgcccc gcaggagtcg caacacgagg ccgcctatgc acagtggttc gacgaggttg
     2761 cccagcgtcg cgaaggtcgg cgcgaacgac tcgccgaggc atctccgttt ctacccatgc
     2821 cgctgtggtt cgcgctgggg gtcggggcga cgctcaccct ggcatacatg gtcgtccagg
     2881 ccgacaagcg cgagaagatg gtgattcagg ccattccaat cggattcgtc gccgcgctca
     2941 ttactgcggg aatgctcgtg gtcgtcttcc tcgacaaccc gtacaccggc ggaaacggca
     3001 gcattaagcc ggcggagatg aatcgcacgc tcatgcgcat cgacaacggc ggccaagttc
     3061 cgtgcgacca gcggggtaac cctacctagc cgtctgaacg ctcggggcgg acgtgctcgg
     3121 gctggacgtg ctcggcgtgc ttggggccgc ggagaccttc tgcgcgacga attccgccgc
     3181 ctgatcggtc agaccgttcg cggggtacat gttgtgcgca ttgctgtctc cgccgcccgg
     3241 cgagcagatc ggatcctccg ggatgcacag gtcgacggtc ttcgccgtgt agagatgtcc
     3301 gacggtgatc ggcggtgcgg tgttggcgat catctgcagg aatccgctcg agggcttgcc
     3361 gaacagcgca accgcggcga cgtggtcggc gatggcgggc ggcatcggcc cggtcagacc
     3421 ggcgggcatc tggaaacctg cgggtaccga gtcagcggtg agatatccgg tgacggccgc
     3481 accctgcgag taaccgctga gaacgatctt ggtcttgggg cactcggcgg cgaccgcctg
     3541 aaccttgttg ctcgcgtcga tgacgccgtc agcggccgtc tggaaatcga gcgacgccgg
     3601 gtattccacc ggatacacgt ccaaggactt gtcgccgagt ttggggcgca gcgagtcgac
     3661 gaatgcctgc ccggtcccgc cgacaccggg cggctcgaag gtgccgcgtg cgaagacgag
     3721 ctcgacgtcg gggcaaggcg cagccgaagc ggcgggagca gtgaggaaag gagcgaagac
     3781 cgtggccgcg acaactccgg tagctgcgaa caaacgactg acgacgatca acttgaaccc
     3841 accgcccccc ttattggttc tcgaccttag gcggggcttc atcgcactcc gccttgcatg
     3901 cctacccaac ggctgtgtct acaaacttta gtccgtcgac gtaagccgcg tcatcccgga
     3961 agtggtgaaa attctcgctg cgtgggcgaa gcctggttcc gtcgcgcggc accctgtcgc
     4021 ctgctgaccg agcagcggaa gtcggtgacc gcatcctggc gcccggaacg gatgtgctcg
     4081 gggccgattc atcccgaggc cggacatccc ctcctagaat gctcacagca ggtagttagg
     4141 caccgaagga agaggaccgc acgcatggtg aacttttcgg ccccggctcg acggcttctc
     4201 cccgcggctg tgctggcttt ggcagtcgtg ggcgcaccgg cggtggcggt gatggcgatg
     4261 ccgggtgcag tgattgccac gccgctcgcg gcgtgcacga gtggtgagga agaggacgtc
     4321 tacaccacat cgtgcgtgcc cttcatggtg cccaactcgc ccgacgacag cggattcacc
     4381 acgaacgcgg ccaaccccga catcccggag atcgacggcg tcccgtgcac cggccatgac
     4441 agcggggcct gcatcggcct gtcggaggag cagcaggaca tgggcccgca gccggtgccg
     4501 cgctcgacca tcagttccag cccgta
//
LOCUS       DASYLX010001128        11571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2719986,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001128 DASYLX010000000
VERSION     DASYLX010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719986"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VGP27_26890"
     CDS             complement(<1..487)
                     /locus_tag="VGP27_26890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV7854960.1"
                     /translation="MKFGISTFVNDDTIDTVSLARAIEERGLESLVVAEHTHIPASRE
                     SPYPEGGELPSFYYGTLDPFVTLAAAAAVTSKIELMTGIALLIQRDPIITAKEAASID
                     VISGGRFALGVGAGWNIEELRDHGTDPKSRGALLDERIEAIKKLWTEEPAEYHGKYVH
                     FD"
     gene            1111..1476
                     /locus_tag="VGP27_26895"
     CDS             1111..1476
                     /locus_tag="VGP27_26895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854961.1"
                     /translation="MSPHVKEVRHMRKERQEVIHINTDWRGRAKPGPWYGRYYEDWDA
                     AHPKAWVRGQAVPGRFYAGALWGSSTLAWDTAWFRAQRVKLPKGSHHVVPGRDKIPLS
                     DKLDPTDTGVMRLQFRQIR"
     gene            complement(1646..1867)
                     /locus_tag="VGP27_26900"
     CDS             complement(1646..1867)
                     /locus_tag="VGP27_26900"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854962.1"
                     /translation="MSEAKRAEEGVTRADGTKFGRPRKVADTEHISTAKRMKADGHTG
                     KDIAKYLGVSRATLYRYLAEGTSHDQIVA"
     gene            complement(2205..3002)
                     /locus_tag="VGP27_26905"
     CDS             complement(2205..3002)
                     /locus_tag="VGP27_26905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7854963.1"
                     /translation="MGRLEPGYVFDTAWEHERERLAANERYWDPFSIRILEQLGIAEG
                     MRVLDVGAGGGTIARWFARHVAPSGSVLAVDLDPRFIMALDEPNLEARQMDLFADDFP
                     RDSFDVVHARCLLEHLPERIRGLERLTCAARPGGLVVICDFGGELGGPLCPTPDDLLA
                     QEVFLAVVNEAGWDHTWAPTIAEHMRRFGLEEVDAESFRRYTVGGDVGSVRINQFSLG
                     VVRDRMVATGRITDGQIDAHLARLRDPTVGHLSFETWYAWGRRPVAT"
     gene            complement(3122..3349)
                     /locus_tag="VGP27_26910"
     CDS             complement(3122..3349)
                     /locus_tag="VGP27_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7854964.1"
                     /translation="MAALERDTMIVRTRAGLAAAAANGRKGGRPRKVDDAAAAKARQL
                     RDKGITANDIAKMLGVSRATVYRYLATDAAA"
     gene            complement(3355..4365)
                     /locus_tag="VGP27_26915"
     CDS             complement(3355..4365)
                     /locus_tag="VGP27_26915"
                     /inference="COORDINATES: protein motif:HMM:NF025454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HEV7854965.1"
                     /translation="MTTLTGDGDFAPDDDDLSTAITAPPTTGRLVRVPLRQVWAHEAG
                     NFTPWLLANAEVLGEALEMDLEITRAEHPVGKYWLDLIGTDRRTGHRVIIENQFGVSD
                     HGHLGQLITYAAGTEAVNIVWVTEGLREEHRAALMWLNDRTSTETRFFGVEVTAVRIG
                     DSVPAADFRPVVMPNDWEKVVRTTVQAQSVREEMYAEFWQLFRDGLACEGLSDWTTAK
                     KSLGNWFELRAGTSNAVYGIAFGAARMRSELTFNSNDVIVNEHRYQQLFKARSAMIAA
                     YGSELTFEAKDGIKQRRIADYRSGSIENRDEWADYVAWSIDSQRRLRRAVDAAGGMPA
                     SG"
     gene            complement(4427..4885)
                     /locus_tag="VGP27_26920"
     CDS             complement(4427..4885)
                     /locus_tag="VGP27_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV7854966.1"
                     /translation="MSGSRDDRPGLAQLLAYVREHDTVVVWKLDRLGRNMLHILQTVK
                     ELTDRSVTLVSTSDGIDSSTPAGRMMIGVLGSLAEYERELVKERTALKRAASRAGGTR
                     FGRPRKVDNADNIATAKRMKADGHTGKDIAKFLGVSRATLYRYLAEGNAA"
     gene            complement(5188..5535)
                     /locus_tag="VGP27_26925"
     CDS             complement(5188..5535)
                     /locus_tag="VGP27_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HEV7854967.1"
                     /translation="MAVDGLPEIDVARVQRWCAGQVPEHVRDEVRVECDVAPRHLTIC
                     ECRPPWREDFGPDWTRFPIARLHYTKATGLWTLYWRDRNEKYHRYNPLEPSPQVQDLL
                     DYLDGRSDPIFWG"
     gene            complement(5569..6747)
                     /locus_tag="VGP27_26930"
     CDS             complement(5569..6747)
                     /locus_tag="VGP27_26930"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV7854968.1"
                     /translation="MEVGVGSLGFDVEQQALIWAMWRRGDAIREMERTLGETLPRIRR
                     YLRESGGMPPIPRRRKVGHLTLEEREEISRGIAAGLSARAIAGRIDRPSSTVSREIAR
                     YGGRDVYRALVADATAFQRARRPKPSKLATNPELRDVVADKLDDDWSPQQIAEWLRRE
                     FRGDASMRISHESIYRDVYMPSRKVFDATKFHRLRSKRSIRRPRGKKRSHGRGQIRNM
                     ISIRERPIEADTRRVAGHWEGDLVLGARPSAVATLVDRASRYAMVVALPDGNKADAVA
                     AALIDHMGRLPAHLRRSLTWDRGSEMAQHSAITAALGLPVFFCDPHHPWQRGTNENTN
                     RLLRQYLHRNADLATFTQDELNAVAAKLNHRPRRVLGWSTQAEAFGATEPIPMLVAPA
                     "
     gene            complement(6796..7233)
                     /locus_tag="VGP27_26935"
     CDS             complement(6796..7233)
                     /locus_tag="VGP27_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854969.1"
                     /translation="MGLVVAQGSGNRLNETYLAQALARLEAEHGDPLAALDYTALAIR
                     DHHDSGNTIQVRAELGTLAALFNRLGNYEVAAIMSGFADMPYTRSVVAEINATIAHLR
                     EVLGDQTYESLAREGESMTTAEVASYAYDQIDQAREKLNGVSK"
     gene            7466..7951
                     /locus_tag="VGP27_26940"
     CDS             7466..7951
                     /locus_tag="VGP27_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854970.1"
                     /translation="MTRAADTVHRAIAASGGLGEPWCCGGDQWPDAVRVGNRLMVVET
                     DSDTYGTPAGLGWSITLYVVPDDGGAAAPVAVLAEDNGDAVRTLTSLIRQTGRPLTWT
                     RERPGRYRSGEYLVGQLDTSEWFAEGPGIDRCFDHKAAAQAACSSRNHCQREQIIGGD
                     N"
     gene            8058..8480
                     /locus_tag="VGP27_26945"
     CDS             8058..8480
                     /locus_tag="VGP27_26945"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEV7854971.1"
                     /translation="MKVLKRAVALLMAVLGLLTGASALASAAPGAQGDVTYPQLLDQN
                     GVSSTNPEAMVSYAHDLVDQLSASPQNDASVGTIVSTGEERISQHDMGVIIAAAVVAY
                     RPSLIPVVRHWADSAAGYPCGAAAEAVLGRGGQDIDHG"
     gene            8584..9192
                     /locus_tag="VGP27_26950"
     CDS             8584..9192
                     /locus_tag="VGP27_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854972.1"
                     /translation="MSWPNCEARIPASATFGIIGVTEGTPYSATNPCLAAQAAQFPNT
                     LSLYANSGWNDHSTHLDPSSPKGCLPEDADCLAYNYGYNAGLAAYDAATAAGVSSGMW
                     WLDVESDNTWNDDANQNRNSLQGEDDALTARGATTVGVYSTPSGWQTITGDWQNSWPS
                     WIGADDPASAQGRQACTGHKFTGGPTLVIQSDPHRIDRDIAC"
     gene            complement(9382..9804)
                     /locus_tag="VGP27_26955"
                     /pseudo
     CDS             complement(9382..9804)
                     /locus_tag="VGP27_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725823.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(9801..>11571)
                     /locus_tag="VGP27_26960"
     CDS             complement(9801..>11571)
                     /locus_tag="VGP27_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEV7854973.1"
                     /translation="AGNPFFAGEMVRDLAERGVLRGERGAYISAAEAAEVNVPATLHA
                     TIAARIDRLDPNAKRTLSAAAVVGSRFGLDLLTVLGVEPVVADLVAAQFIDQVRFTRQ
                     PEYVFHHPLIRAVAYESQLKSDRADLHRRLAAVIEARSPASADENAALIAEHLQAAGD
                     RHAAFNWHMRAATWATNRGIAAARLSWERAEKIADALPADDPDRAAMRIAPRTMLCGT
                     AWRVNVNVAGARFEELRQLCTAAGDKASLAIGMAGLVIDNGFHDRVREASRLASEAMA
                     LIESLGDPTLTVGLSFAPIYTKAESAEWGDVLLWSQSVIDLADGDPSKGNFIIGSPLA
                     LALTTRAVARYWLGHAGWRDDLQHGLAMARSADPTSYAAVVTYVYMPAIPYGVVRSDV
                     SVVREIEEALRLAERSSDDFAVDLARVTLGFALAHHQTSADRDRGQTLLAEFSEVFQR
                     QGHNLAEVPLIEVYAARERARRGDRDDAIPLIRAAVDHLFREGQLLAWGIPATGVLVE
                     TLLEHGADDGVAEAEAAIERLAAAPTDEGLVLRDIWLLRLRALLAKAHGEEASYCDYR
                     DRYRDMARTLGFEGHIAWAEAMP"
BASE COUNT         2075 a         3764 c         3626 g         2106 t
ORIGIN      
        1 cgtcgaaatg gacatacttg ccgtggtact cggccggctc ttcggtccac agcttcttga
       61 tcgcttcgat tcgctcgtcg aggagcgcgc cgcgggactt cggatccgtg ccgtggtcgc
      121 gcagctcctc gatgttccag ccggcaccaa ccccgagggc gaatcgacct ccggagatga
      181 cgtcgatgct cgcggcttcc ttggcagtga tgatcgggtc gcgctggatc aacagcgcaa
      241 tcccggtcat cagctcgatc ttcgacgtga ccgccgccgc tgccgccagc gtgacgaacg
      301 ggtcgagtgt cccgtaatag aatgacggca gctcgcctcc ctcgggatac ggagattcgc
      361 ggctcgccgg aatgtgcgtg tgctccgcta cgacgaggga ctcgaggccc cgttcctcga
      421 tagctcgcgc caaggacacc gtgtcgatgg tgtcgtcgtt gacgaatgtc gaaattccga
      481 acttcatcgc cacctccgag tgcgtttact gcggcgttcc gacaccgcag acgcgaaggg
      541 tattccccgc cgccagagaa acatccctca ttggactcaa tccgcgagtt gatccgacgg
      601 cgggaatagt gcggtgtgtg tcgtggtact acctccgcgt gaatatagat ctgagcggaa
      661 agacggccct tgtcacaggc ttgacccagg gcatcggctt cgcgatgatc tttgactcag
      721 acctgtattc accgtgggct gtcagtgggg ctggggcatc cggtgggatg ggaaggcccg
      781 gacaggcttc gatgtagtcg atgtccggcg acacccaatc cgccactgct ggcggctcat
      841 gtcggtcctg agcttggcgc acatcgccga cgtggggtcg gcgtacgtgg ccacagccgc
      901 agtgccttgt cggcacctcg ggaggcgatc cgcgtaccgt cggggctgaa cgccacgctg
      961 tacacatcgg gctacgtgga gtgcgcctac ggcggcctcg gggaagggtg ccgcagcggt
     1021 atgacttggt cccttcatgg acctgcccga cgactggttc accatggacg ggaacagcta
     1081 ctgctccgag cactggccga tggcgcggcg atgagtcccc acgtcaagga ggtgcgccac
     1141 atgcgcaaag aaaggcagga agtgatccac atcaacaccg actggcgggg aagagccaaa
     1201 cccggcccct ggtacggccg ctactacgaa gactgggacg ctgcccatcc gaaggcgtgg
     1261 gtacgcggac aggcggtgcc cggccggttc tatgcgggcg cgctatgggg atcctccacg
     1321 ctggcctggg atacggcctg gtttcgtgcg caacgggtga agctgcccaa gggctcgcac
     1381 catgtggtgc ccggccgtga caagataccg ctgagcgaca agctggatcc caccgacacc
     1441 ggggtgatgc ggctgcaatt taggcagatc cgatgagcta cagggcctat cacggctacg
     1501 acgaccgcga cgtcatcacc tccgagggaa gaattagagc gatttgctca cccactgcct
     1561 tgagaagtcc acatgctgtg gaagatgcag cacgaccgcg gttgagggtt ccggcgagtt
     1621 cagagctatg gggaccgacc cgaacctagg caactatctg gtcatgcgac gttccctcag
     1681 ccaagtatcg gtacaaagtt gcccggctca ccccgagata cttcgcgatg tccttccccg
     1741 tgtgtccgtc ggccttcatc cgcttcgctg tagagatgtg ttcggtatcc gccaccttgc
     1801 ggggccgtcc gaacttggtg ccgtcggcac gggtgacgcc ctcttcagcg cgcttcgctt
     1861 ctgacactgc tcgcgctcgc caagcaggtg tcatttctaa acattgcgga cctgtgcggg
     1921 gccgcgcggc agtcatcagg acagcccagg tcgaacaatc cgatttccca gtgtcacagt
     1981 cagtccgagt tctcgtcgat ctgacaccgg atgaattgtc ggaccggtat gcgagtcaaa
     2041 ccacaggacg agctaactta ctagcgcgac gcgccgtcgc atattcgccc cgccgctact
     2101 agccccgttg tggcacaaca tgttttcgct aacggcgatc aaatctgatg cgcgagctgc
     2161 aacacttttg cccaatactt ttgtgctcag acttccgcgt ggatctatgt agcaaccggt
     2221 cgcctacccc aggcatacca tgtctcgaag ctgaggtgtc ccaccgtggg gtcgcgtagg
     2281 cgtgccaggt gggcgtcgat ctggccgtca gtgatcctcc cggtcgccac catgcggtcg
     2341 cgcaccacgc ctaacgagaa ctggttgatc cgaacgcttc cgacgtcgcc accgaccgtg
     2401 taacggcgaa acgattcggc atcgacttcc tccaggccga atcggcgcat gtgctcggcg
     2461 atcgttggtg cccaggtgtg atcccaaccc gcctcattga cgaccgccag gaacacctct
     2521 tgggcgagca ggtcgtcagg cgttgggcag agcgggccac cgagttcgcc gccgaaatcg
     2581 cagatgacca ccaaaccgcc cggacgagcg gcacaggtca aacgctccaa gccacggatt
     2641 cgctcgggca gatgctcaag gaggcaccgc gcgtgaacca catcgaacga atcacgcggg
     2701 aaatcgtcgg cgaacagatc catctgccgt gcttcgaggt tcggctcgtc gagcgccatg
     2761 atgaaccgtg ggtccagatc gaccgccagc accgagccgg acggcgcgac gtgacgggca
     2821 aaccatcgcg cgatcgtgcc gccgccagcg ccgacatcga gaacccgcat tccctcggca
     2881 atcccaagtt gttcgaggat ccggatcgaa aacgggtccc aatatcgctc gttcgccgcc
     2941 aggcgctccc gttcgtgctc ccaggccgta tcgaatacgt aacccggctc caggcggccc
     3001 atgccgcgag attactgcgg atctattggg aatgcgcgca aatacctgct cccgagagcg
     3061 agttcgctga gctagcgacg cccgaaaccg gaatccgact aaaggctcca gaccggcggt
     3121 ctcacgcggc ggcatccgtg gcgagatacc ggtacacggt ggcgcgggag acccccaaca
     3181 tcttcgcgat gtcgttggcc gtgatgccct tgtcccgaag ctgacgggct ttggcggcag
     3241 cggcatcatc gaccttgcgt ggccgcccgc ccttgcgacc gttggctgcg gcagcggcca
     3301 gcccggcgcg ggtccgcacg atcatggtgt cgcgttcgag ggcggccacg gggttcagcc
     3361 agacgctggc atgccaccgg cggcgtcaac agcgcgtcgt agacgtcgtt gggagtcgat
     3421 cgaccaagca acgtaatcgg cccattcgtc acggttctcg atcgagccag atcggtaatc
     3481 cgcgatacgc cgctgcttaa tcccgtcctt agcttcaaag gtgagttccg atccgtaggc
     3541 ggcgatcatg gctgagcgcg ccttgaataa ctgttggtag cgatgttcgt tgacgatcac
     3601 gtcattggag ttgaaggtaa gctccgagcg catcctcgct gcaccgaaag cgatgccata
     3661 caccgcgttc gacgtcccag cccgaagttc gaaccagtta ccgagtgact tcttggcggt
     3721 tgtccaatcg ctaagcccct cacatgctag accgtcgcgg aatagctgcc agaactcggc
     3781 atacatctct tcgcgaacgc tctgcgcttg caccgttgtg cggacgacct tctcccaatc
     3841 gttcggcatt acaactgggc ggaagtcggc ggctggcaca gagtcgccga tgcgcacagc
     3901 ggtgacttca acaccgaaga agcgggtttc ggtgcttgtt ctgtcgttca gccacatcaa
     3961 cgccgcgcga tgttcttcgc gcaaaccctc cgtaacccag acgatattca ccgcttcagt
     4021 acctgccgcg taggtgatca actgtccgag atgaccatgg tctgagacgc cgaattggtt
     4081 ctcgatgatg acgcgatgac cggttcgacg gtccgtgccg atcaggtcta accagtactt
     4141 gccaacgggg tgctctgccc gagtgatttc caagtccatc tctagtgcct ctccgagcac
     4201 ctccgcgttc gcgagtaacc aaggtgtgaa attacccgct tcatgcgccc acacctgtcg
     4261 cagtggtacc cgaacaagcc gcccagtcgt cggtggggca gtgatcgcgg tcgagaggtc
     4321 gtcgtcatcg ggggcaaaat cgccgtcgcc agtgagcgtc gtcatgtcgg cagcgtaggc
     4381 caccgctgct tcagcggatt cgcccatctc acgcgaggct cgtccgtcag gcagcattgc
     4441 cctcggccag gtaccgatag agagtcgccc ggctgacgcc taagaactta gcgatgtcct
     4501 tccctgtatg gccatcggct ttcatccgct tggcggtcgc gatgttgtcc gcgttatcga
     4561 ccttcctcgg gcgtccgaac ctggtgccac cagcgcggga agcggcccgc ttcagcgcgg
     4621 tgcgttcctt caccagctca cgctcgtact ccgcgagcga ccccagcaca ccgatcatca
     4681 tgcgacctgc cggagtcgac gagtcgattc catcggaggt cgaaaccagc gtgacgctgc
     4741 ggtcggtcaa ctccttgacg gtctgcagga tgtgaagcat gttgcggccc aatcggtcca
     4801 gcttccatac cacgacggtg tcgtgctcgc ggacgtaggc caacagctgc gccaacccgg
     4861 gccggtcgtc gcgagagccg gacataacgt cgaaaagacc ttggctaccc ctgcggcggc
     4921 gagcgcttcg ttctgttgat ccaaggtttg ggcaacggtg gagacacggg tgtatccaat
     4981 gcggttgccg gtggcggccg cgcgttcctt gttttcctgt ctcacaatgc ttttgaacgc
     5041 caatacgcga cattccattc cgagactaac ttctgagaca ccgcgacccg gaccaacgag
     5101 cgtgcaagaa acgactcgac cgatgtctca tatgaggtct tgcgctcaac cggtcagtgc
     5161 atcgggttgg gtcaggttag ccggtcgtta gccccagaag atgggatcag atcggccatc
     5221 gaggtagtca agcaagtcct ggacttgtgg gctgggttcc agagggttgt agcggtggta
     5281 tttctcgtta cggtcgcgcc agtacagcgt ccatagtccg gtggccttgg tgtagtgcag
     5341 ccgggcgatt gggaatcggg tccagtcggg accaaagtcc tcgcgccagg gcggtcggca
     5401 ctcacagatg gtcagatgtc ttggcgcgac atcgcattcg acgcggacct cgtcgcgaac
     5461 gtgctcggga acctggcccg cgcaccaacg ttgcacgcgg gcgacgtcga tctcgggaag
     5521 tccgtcaacg gccactgagc catgatcgca gccgagaaac cgcaccggct aggcaggcgc
     5581 aaccagcatc ggaatcggct cggtagcgcc gaacgcctcg gcctgagtgg accagcccag
     5641 tactcgacgc gggcggtggt tgagtttggc ggcgacagcg ttgagctcgt cttgggtgaa
     5701 cgtggccaga tctgcgttgc ggtgcaggta ctggcgcagc agtcggttcg tgttctcgtt
     5761 ggtgccgcgc tgccatgggt gatgcggatc gcagaagaac accggcagtc ccagcgccgc
     5821 ggtgatagcc gaatgctggg ccatctcgct gccgcggtcc caggtcaacg accgccgcag
     5881 gtgtgctggc agccggccca tgtggtcgat cagcgcggca gccaccgcgt cggccttgtt
     5941 gccgtcgggc agcgccacga ccatcgcgta acggctggcg cggtcaacga gggtggccac
     6001 cgccgatggg cgcgctccca acacgagatc gccctcccag tggccggcca ctcgccgggt
     6061 atccgcctcg atcggtcgct cacggatgga gatcatgttc cggatttggc cgcgaccgtg
     6121 cgaacgcttc tttccgcgcg gtcgtcggat cgaacgcttg cttcgcagac ggtggaactt
     6181 ggtggcgtca aatactttcc gcgacggcat atacacgtcg cgatagatcg attcgtgaga
     6241 gatccgcatc gacgcgtcac cgcgaaactc ccgccgcagc cactcggcga tctgctgcgg
     6301 cgaccagtcg tcgtcgagct tgtcggcgac cacatcccgt aattcggggt tggtggcgag
     6361 cttgctcggt ttgggtcgcc gcgctcgctg gaacgccgtt gcgtcggcga ccaatgcccg
     6421 gtacacatcg cggccaccat agcgggcaat ctcccgcgac accgtcgacg acggccggtc
     6481 gatacgtccg gcgatcgccc gtgccgacaa gccggccgcg atcccgcgtg agatctcttc
     6541 acgttcttcc agggtcagat gaccgacttt gcgccgacgc gggatcggcg gcatcccgcc
     6601 cgattcacgc aagtagcgtc ggatccttgg cagcgtctca cccagcgtgc gctccatctc
     6661 gcggatcgca tcaccacgcc gccacatcgc ccaaatcagc gcttgctgtt cgacatcaaa
     6721 tccaagggac cccacaccaa cctccatcgg cacatcatcg gctgcagtga tgcactgact
     6781 ggttgaactc aaggtctatt tcgagacgcc gtttagtttt tctcgggctt ggtcgatttg
     6841 gtcgtaggcg tagctggcta cctcggcggt ggtcatcgat tcaccctcgc gggcgagcga
     6901 ctcgtaggtc tggtcgccga ggacctcgcg caggtgggcg atcgtggcgt tgatctccgc
     6961 cacgactgac cgcgtgtagg gcatgtcagc gaatccgctc atgatcgcgg cgacttcgta
     7021 gttcccgagc cggttgaaaa gggcggcgag ggtacccagt tcggcacgga cttggatggt
     7081 gttgccggaa tcgtggtggt cgcggatcgc cagggccgtg taatcgagcg cggccagcgg
     7141 gtcgccgtgt tcggcctcca gtcgcgcgag agcctgcgcc aggtaggttt cgttaaggcg
     7201 gttgccgctg ccttgggcga ccaccagacc caccgcagcg cgtcacgcgc gcggtcagga
     7261 tcggcgtccg cggaagcgaa gccatacgct aggtgcgcgc cacgaactga gaatccgcca
     7321 cgccaatcgt ctcccatggc atgctggagc gctgtgcctc agcggggcgt tcatcgcgcg
     7381 ccacgtccac gttctgggat ttcggctgac ctgttcgtcc ctcgacttcg cggagctgtt
     7441 ctaatttcgc ctcctcgact cagcaatgac ccgcgcggct gataccgtgc atcgtgccat
     7501 cgcggccagc ggtggcctcg gcgagccgtg gtgctgcggc ggcgaccagt ggccggacgc
     7561 tgttcgcgtc ggcaaccggc tgatggtggt cgaaacggac agcgacacgt acggaacacc
     7621 ggcgggtctc ggctggtcga tcaccctcta cgtggtgccc gacgatggtg gggctgccgc
     7681 acctgtggcg gtcctcgccg aggacaacgg cgacgcggtt cggacgctca cgtcgctaat
     7741 ccgtcagacc gggcgaccgc tgacgtggac acgggagagg ccgggccggt accgatccgg
     7801 cgagtacctc gtcggacagc tcgacaccag tgagtggttc gccgaaggtc cgggcatcga
     7861 tcggtgcttc gatcacaagg cggcggccca ggccgcgtgc agcagcagga accactgtca
     7921 gcgtgagcag atcatcggcg gtgacaattg aacttaacct gcgctggcat gatttcacgc
     7981 gctgtctctg ggttgatcac ctcgatagag gcggggtcat cctccagctc gtgccgcgca
     8041 cgtgaaagga gaccatgatg aaggttctga agcgagcggt agcgctgttg atggcggtac
     8101 tgggcctgct gaccggcgct tcggcgctgg cgtcagcggc tcccggcgcc caaggcgacg
     8161 tgacttaccc gcaattgctc gaccagaacg gggtgtcctc caccaatccc gaggcgatgg
     8221 tctcttacgc tcatgacctc gtggaccaac tgagcgcctc cccgcaaaac gacgccagtg
     8281 tggggacgat agtcagcacc ggcgaggagc ggatttctca gcacgatatg ggcgtgatca
     8341 tcgccgccgc agttgtcgcc tatagaccgt ccctgatccc cgttgttcgg cactgggcgg
     8401 acagcgcagc tggctacccc tgtggcgccg cagctgaggc ggtgctggga cgcggtgggc
     8461 aagacataga ccatggttaa gcgactcgcc tgggtggcag ccgccgcctg ttgcctgctg
     8521 caatccgctg tggggattgc cagcgcagcc acgctgtacc cgcccgggag caccggagtc
     8581 gacgtgagct ggccaaactg cgaagcgcgg attccggcca gtgcgacgtt cgggatcatc
     8641 ggcgtgaccg aaggtacgcc ctattccgcc accaatccgt gccttgccgc ccaggcggct
     8701 caattcccga acacgttgag cctgtatgcc aacagcggct ggaacgacca ctccacccat
     8761 ctggacccat ccagccccaa agggtgcctg cctgaggatg ccgactgcct ggcctacaac
     8821 tacggataca acgcaggcct ggccgcctac gacgcggcca ccgcggccgg agtgtccagc
     8881 ggcatgtggt ggctcgacgt cgagtcggat aacacctgga acgacgacgc gaaccaaaac
     8941 cgcaacagcc tgcaaggcga agacgacgcc ctgaccgccc gcggtgccac caccgtcggc
     9001 gtctacagca cgccatcggg atggcagaca atcaccggcg actggcagaa cagctggccc
     9061 agctggatcg gcgctgatga tcctgccagc gcccagggca ggcaagcgtg cacgggtcat
     9121 aaattcaccg gcggaccgac gctggtgatc cagtcagatc cgcaccgaat cgaccgagac
     9181 atagcctgtt gacgaccacc cggtcggtac tacgcgaggg ctcgtcactg cgcgtgttgt
     9241 cgcacggcga ggccgatggt gaagctcggc aatttcgacg gccactgccg gatgggctcc
     9301 tcggcccagg gctgtgctgc cgacgaggct tccgcggcgg cttcggaggc ggaaccagcc
     9361 agccgacacg taatcgccgt gccccaatgc aatcaggtcg atgctgctgc acagcccggc
     9421 agtggtgccg tcgagcagga tctgaccacc gtgtccggca gccatcactc tcgcggcccg
     9481 gttcagaacg gctccgaagt agtcgccgtc ccgcagctcg gcctcgccgg tggccagccc
     9541 catccgcacc ggcaacttca gctctcgctg tgcggccacg gcagcatcga ctgcggactt
     9601 cggcgatgag aatgccgcgc acacaccgtc accggtgtgc ttgaacaaga agccgtcgtg
     9661 tttctcgatc gcggtgcgca gcacctcgtc gtgcgcggct agagccgtac gcatcgcatc
     9721 cgcgtcggct tcccaccgac gggtcgaccc ctccacgtca gtgaacagaa aggtcaccac
     9781 cccagaaggc ggactcgccg ttatggcatc gcctcggccc acgcgatatg tccttcgaag
     9841 ccaagcgttc tcgccatgtc gcggtagcgg tcccgatagt cgcagtagct tgcctcctcg
     9901 ccgtgagcct tggccagcag ggcgcgcagc cgcaatagcc agatgtcgcg caaaaccaga
     9961 ccctcgtcgg tcggtgccgc cgctaatcgc tcgatcgcgg cctcggcttc ggccacgcca
    10021 tcgtcggcgc cgtgttcgag cagtgtctcc actagaacac ccgtcgctgg aatgccccac
    10081 gccagcagtt gcccctcgcg gaacagatgg tcgacggcgg cgcggatgag cggtatggcg
    10141 tcatcgcggt ctccacgccg ggccctctca cgtgccgcgt agacctcgat gagcggtacc
    10201 tcggccaggt tgtgtccctg gcgttggaac acttcgctga actcggcaag gagcgtctgt
    10261 ccgcggtcac gatccgcact cgtttggtgg tgcgccagcg caaagcccag cgtcacccgg
    10321 gcgagatcca ccgcgaagtc gtcactggat cgttcggcaa gccgcagggc ctcctcgatc
    10381 tcgcgcacca cagaaacgtc ggacctcacc acgccgtacg gtattgctgg catgtagacg
    10441 taggtgacga ccgcggcgta ggacgttggg tcggcgctgc gggccatggc caggccgtgc
    10501 tgcaggtcgt cgcgccaccc ggcatgaccc agccaatacc gggcaaccgc ccgcgtcgtc
    10561 aaggcgagtg ctagcggaga cccgataatg aagttgcctt tcgacgggtc accgtcagcc
    10621 aggtcgataa cgctttgtga ccagagcagc acgtcacccc actcggcgct ctcagccttg
    10681 gtgtagatgg gcgcgaagga cagccccacc gtcaaggtcg gatcgccgag cgactcgatg
    10741 agagccatgg cttccgatgc cagccgcgac gcctcgcgca cccggtcgtg gaacccgtta
    10801 tcgatcacca gccccgccat cccaatggcc agtgacgcct tgtccccggc ggcggtgcac
    10861 aactgccgca gttcctcgaa ccgggccccg gcaacgttca cattgactcg ccaggcggtc
    10921 ccgcacaaca tggtgcgcgg ggcgatgcgc atggctgctc ggtccgggtc atcggcgggt
    10981 agtgcgtcgg cgatcttttc cgcacgctcc caactcagtc gcgccgcggc gatgccgcgg
    11041 ttggtcgccc acgttgcggc ccgcatgtgc cagttgaacg cggcgtgccg atcaccggcg
    11101 gcctgcagat gttcggcgat cagcgcagca ttttcatcag ccgaggccgg gctacgggct
    11161 tcgattacgg cggcaagccg ccgatgcaag tcggcgcgat ccgacttgag ctgcgactcg
    11221 taggccaccg cacgaattag cgggtggtgg aacacatact ccggctgtcg ggtgaacctc
    11281 acctggtcga tgaactgggc cgccaccaga tcagcaacca ccggttccac tcccagcaca
    11341 gtcagcaggt cgaggccgaa ccgggagccg accaccgcgg cggcgctcag cgtgcgtttc
    11401 gcgttcgggt cgagccgatc gatgcgagcg gcgatcgtcg cgtgcagcgt cgcaggcaca
    11461 ttcacctccg cagcctctgc ggccgagatg tatgcgccac gctccccgcg caggacacca
    11521 cgctcggcga ggtcgcgcac catctccccg gcgaagaacg gattaccggc g
//
LOCUS       DASYLX010001129         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1292884,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001129 DASYLX010000000
VERSION     DASYLX010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292884"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGP27_26965"
     CDS             <1..336
                     /locus_tag="VGP27_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7854974.1"
                     /translation="LWSQDTRPEGYSWIDANDSANNVLSFLRFGDDGSVLACVFNFSG
                     SEHSRYRLGLPHAGTWREVLNTDADIYNGSGIGNFGAVEATNEPWHGRPASALMVLPP
                     LAALWFEPA"
     gene            complement(404..1318)
                     /locus_tag="VGP27_26970"
     CDS             complement(404..1318)
                     /locus_tag="VGP27_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7854975.1"
                     /translation="MTRPRPPAGPGPSIAGAVDLSVLKQRATPAGEAGVPAQGSGAPG
                     GVEVTEANFEAEVIGRSNEVPVVVLLWSPRSDASVQLGEALASLAAADGGKWSLATVN
                     VDTTPRVAQVFGVEAVPTVVALAAGQPLSSFQGMQPPDQLRGWIDSLLNATAGKLEGS
                     ADADEPEQVDPELAQARAHLDAGEFDAAQAAYQKILDANPNHEEAKGAVGQIAFLTRA
                     SARQPDAVTAADAAPDDIEAAFAAADVELLQQNVAAAFDRLIALVRRTAGDDRTSVRT
                     RLVELFDLFDPADPEVIAGRRKLANALY"
     gene            complement(1332..1715)
                     /locus_tag="VGP27_26975"
     CDS             complement(1332..1715)
                     /locus_tag="VGP27_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEV7854976.1"
                     /translation="MAAMTSALDLRTRVGWFRLIALAEAVSWVGLLIGMYFKYLGTPR
                     TEIGVKVFGMTHGLVFIAFVVAGVLVGIAVNWTLATWLLALLASVVPLCSVIFLIWAD
                     RTGRIGAARVAAGVLQPGRSLPETT"
     gene            complement(1760..>2372)
                     /locus_tag="VGP27_26980"
     CDS             complement(1760..>2372)
                     /locus_tag="VGP27_26980"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7854977.1"
                     /translation="KDGVYADEVVPVKIPQRKGDPLEFTEDEGIRANTTAESLSGLKP
                     AFRKDGTITAGSASQISDGAAAVVVMNRDKAEQLGLKWLVEIGAHGVVAGPDSTLQSQ
                     PANAIKKAVAREGIAVEQLDVIEINEAFAAVALASMNELGVNSDKVNVDGGAIAIGHP
                     IGMSGARITLHVALELARRGSGYGVAALCGAGGQGDALILRAG"
BASE COUNT          392 a          821 c          767 g          392 t
ORIGIN      
        1 ctgtggtcgc aagacacccg cccggaaggc tattcgtgga tcgacgcgaa cgattccgcc
       61 aacaacgtgc tgagcttcct gcgcttcggc gacgacggct cggttctcgc gtgcgtgttc
      121 aacttctcag gctccgagca cagccgctat cgactcggcc taccgcacgc gggcacgtgg
      181 cgcgaggtgc tcaacaccga cgctgacatc tacaacggtt ctggcatcgg caacttcgga
      241 gcggtcgagg cgaccaatga gccgtggcat gggcgtcccg cgtcggcgtt gatggtgctg
      301 ccgccgctgg ccgctctgtg gttcgagccc gcctagctac cgcccaaact cacgtttggg
      361 cgtaaaagtg cgagtagacc gcaccatttc gtcgatctcg gcgtcagtac agcgcgttgg
      421 cgagcttgcg cctgccggcg atgacctcgg ggtcggccgg gtcgaagagg tcgaacagct
      481 cgaccagccg cgtgcgcacc gacgtgcggt cgtcgcctgc ggtgcgccgc accagcgcga
      541 tcaaccggtc gaacgccgca gccacattct gctgcaggag ctcgacgtcg gctgcggcga
      601 acgcggcctc gatgtcgtcg ggcgcggcat cggccgccgt gaccgcatcc ggttgccgcg
      661 cggatgcgcg ggtcaggaag gcgatctggc ccaccgcgcc tttggcctcc tcgtggttgg
      721 ggttggcatc gaggattttc tggtaggcgg cctgcgcggc gtcgaactcg ccggcgtcga
      781 gatgcgcacg cgcctgcgcc aattcggggt cgacctgctc gggttcgtct gcgtcggcgg
      841 acccttcgag ttttccggcg gtggcattca acagcgagtc gatccagcct cgtagctggt
      901 cggggggctg catgccttgg aagctcgaca gcggttgccc cgccgccaac gccacgacgg
      961 tcggcactgc ctctacaccg aagacctgag cgacgcgcgg cgtcgtgtcg acgttcacgg
     1021 tggccagcga ccacttgccg ccgtcagcgg cggccaggct cgccagcgct tcacccaact
     1081 gcacgctggc gtcgctgcgt ggcgaccaca gcaggacgac gaccggtacc tcgttggacc
     1141 gaccgatcac ctcggcttcg aaattggcct cggtgacctc cacacctccg ggtgcgccgg
     1201 aaccctgcgc cggcacgcca gcctctccgg ctggggtcgc gcgctgcttg aggacggaga
     1261 ggtcaacagc gccggcaatg gagggcccag gcccggcggg aggacgggga cgagtcacgc
     1321 catcaagtct gtcacgttgt ttccggcaac gatcggcctg gttgcagcac ccctgcggcg
     1381 accctcgcgg cgccgatgcg gccggtccga tccgcccata tgaggaagat cacactgcac
     1441 agcggcacga cgctcgccag caacgccagc aaccatgtgg cgagcgtcca gttgacggcg
     1501 attccgacca acacaccggc caccacaaag gcgatgaaga ccagcccgtg ggtcatgccg
     1561 aagaccttga cgccgatctc ggtacgaggc gtgccgaggt acttgaagta catcccgatc
     1621 agcagaccga cccagctcac ggcctcggcc agagcgataa gccgaaacca acccactcgc
     1681 gtacggagat cgagggcact cgtcatggcc gccattgtgc ccgaatcggc agcgaattac
     1741 tacaaagcgt cgtagggcgc tagcccgccc gcaaaatcaa tgcgtcgccc tggccacccg
     1801 cgccgcaaag cgccgccacg ccatagccgg atccgcgtcg tgccagctca agggccacat
     1861 gcagggtgat gcgtgcaccc gacatcccga tcggatgccc gatggcgatc gctccaccgt
     1921 cgacgttcac cttgtcggag ttcacaccga gctcgttcat cgaggccagc gcgacggcag
     1981 cgaacgcctc gttgatctcg atgacgtcga gctgctcgac ggcgatgcct tcgcgcgcaa
     2041 ccgccttctt gatggcgttg gcgggctgcg actgcagcgt ggagtcgggg ccggcaacca
     2101 cgccgtgcgc gccgatctcc accagccact tcaggcctag ctgttcggcc ttgtccctgt
     2161 tcatcacgac cacggcggcc gcaccgtcgg agatctggga cgccgacccc gcggtgatgg
     2221 tgccgtcctt gcggaaggcg ggcttcaggc cggacagcga ctccgcggtg gtgttggcgc
     2281 gaatgccctc gtcctcggtg aactccagcg ggtcgccctt gcgctgcgga atcttcaccg
     2341 gcacgacctc atcggcgtag acaccgtctt tc
//
LOCUS       DASYLX010001130         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2940842,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001130 DASYLX010000000
VERSION     DASYLX010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940842"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1135..1899
                     /locus_tag="VGP27_26985"
     CDS             1135..1899
                     /locus_tag="VGP27_26985"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV7854978.1"
                     /translation="MERLTGKVAVVTGASRGIGRAVAIRLAAEGAAVAVNYRADRGSA
                     ESLVTELSGRGARAMAFQADVSEPDQIGQLMDSAAQALGGLDILVSNAGIEHFGPLED
                     ITPADFDRVFATNTRGQLFAVQHAARHLGPGGRIVLTSSISAHKSVFHHTIYAASKAA
                     VEAMALNLAAELGQRQITINAIAPGGTNTDMSAKATPHYIHPDLPLDRDTAVKVMSAL
                     GRFAEPDEIAATVAFLVSDDADYITGRTIPIDGGWF"
     gene            2236..2631
                     /locus_tag="VGP27_26990"
     CDS             2236..2631
                     /locus_tag="VGP27_26990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7854979.1"
                     /translation="MPSPIFRRLPIYPPAAWRCREAGIPQTKYHVSGEWQMAEYAEDG
                     MVVRTGSLEIDVPRSLGFFGGIAAGVALGLLEPPLALFIAAVPFVKMLSDQRAPTPMR
                     FIGLLGEGAAKPVGSSGEGTVRLSHEPDK"
BASE COUNT          481 a          905 c          878 g          435 t
ORIGIN      
        1 cggcctccgc ccccgcctcc gctgatggag cgggataccg accgctggca gacctgcggt
       61 ggctgcgacg ctactcgctg caagttgctg ggcatgccct gtaatcgcaa cgacgccaga
      121 tgacgttacg gaacgtggcc gtcaccagcg cgtcgtcctc aagctgactg gccaccgcga
      181 tgctggccga agagtcgggt gcgttcaccc atggcgcgct ccggcaggtg agcgcaggtt
      241 aggcgagtct ccaggaggtt aggcggtctc cagcgagcgc gcgcacgtcc cagcgggcgc
      301 aatcgggggc ctcacccagt cgccgagatg ccggttgccc aaggcggcgg cgaacctgcg
      361 gtttctcaac ctgctctgcg tggcggaccc accgcacggc acgaggtcgg cgacaaacgt
      421 ggacagcgcc tgacgcggtt cgcgctgtac cgccgctgcg ccacaattcg agcagctgcc
      481 ggtggccgtc ggcaaacatc gcgtcgtaca ggtcgagctt cgatgcgaag tacacataca
      541 acgatggctg ccgaagacca acagctacgc cgagatcccg caacgcaaca ccgtccctgc
      601 gaccgaggtc gcctggcgct cggtcacggg tggcgctgct caggttccgg actgctcggg
      661 ctcagcgatc gaatcgaagc gctcggcggc acgcccagga ttgcagccag ccggtaggga
      721 aaatggccgc cgcctgtgaa gacatccgac cgccgaagcg ccagtcttgc tcatggcagc
      781 ccgccacgcc gcagtatgcg gcgccggcga tcgctgagga gggagcgagc gcagagcatc
      841 tgtcgcctcc gggagttgcc agccgggcgt cgctgcccgc gggcaactgc cgctcaagct
      901 cacggacagc aggtcagcat gacgcgtgca tcggagagtc cggttatagc ccggcccgtg
      961 taatcctccc cgacgcacgg gccgggcgcc tcccgactcc tgcgtaggtc aaatgcggtc
     1021 ccgagccctc gatcagccct tgctcatccc cgcggtaggc cgttcagcac acccagtcag
     1081 ggaagtctca tcgcttccgc cagtcgagca cgagaacacg ccgcaggagc agacatggaa
     1141 cgcttgacag gcaaggtcgc ggtcgtgacc ggagccagcc gtggcatcgg gcgcgcggtg
     1201 gccatccggc tggccgccga gggcgcggcc gtggcggtca actaccgcgc ggaccgcgga
     1261 tccgcggaat ctctggtgac cgaactgagc ggtcgcgggg cacgggcgat ggcattccag
     1321 gccgatgtca gcgagccgga ccagataggg cagctcatgg actctgcagc ccaggccctg
     1381 ggcggcctgg acatcctggt cagcaacgcg ggtatcgaac acttcggacc gctcgaagac
     1441 atcaccccgg ccgacttcga ccgggtgttc gccacgaaca cccgagggca attgttcgcg
     1501 gtgcagcatg ctgcccgtca tttaggtcct ggcgggcgca tcgtactgac ctcgtcgatc
     1561 agcgcccaca agtcggtgtt ccaccacacc atctacgccg ccagcaaggc ggccgtcgag
     1621 gccatggcgc tcaacctcgc cgccgaattg ggtcagcgcc aaatcaccat caatgccatc
     1681 gcccccggcg gtaccaacac cgacatgtcg gccaaggcca ccccccatta catccacccc
     1741 gatctgcccc tcgacagaga cacggcagtc aaggtgatga gtgcgctggg ccggttcgcc
     1801 gaacccgacg aaattgctgc caccgtcgcg ttcctggtgt ccgacgacgc cgactacatc
     1861 acaggccgca cgatccccat cgacggtggc tggttctgac cacgctgcgc cgatccagca
     1921 gtgcggcgta cacgacctcg cggtcgagcg gtcgtcagca taatgcgctg gcactggtga
     1981 gactgctggc aagcgcgatc gcgcagacga cttggctggc ttccgccttg tggaacgtta
     2041 agggatgctg tgcagtgatg gggccgggcc ggcactgacg gtccgatgag cacgtcgtcg
     2101 ggaatcagga gggctgccac gatgtcgtcg cggagcagcc cgctcaagca cgacgctgcc
     2161 tattcctggt cggcgagtca gatcatcgct tcctcattgt tagcgacgat catgtgcgca
     2221 tattcatagg tcaagttgcc gagcccgatc ttccgccggc tgccgatcta cccgcccgct
     2281 gcgtggcgat gccgcgaagc gggtattcct cagaccaaat accacgtgtc aggagagtgg
     2341 cagatggcag agtatgccga ggatgggatg gttgtgcgca ccggctcgct cgagatcgac
     2401 gtgccccggt cgttgggctt tttcggcggc atcgccgccg gggtcgccct ggggctgctt
     2461 gagccgccgc tggccctatt catcgcggcc gttccgttcg tgaagatgct cagcgatcag
     2521 cgcgcgccca ccccgatgcg cttcatcggg ttactcggcg aaggcgccgc gaaaccggtc
     2581 ggaagcagcg gcgagggaac ggtccgcctg tcccatgaac ccgacaaata gcactaacgc
     2641 ggataacgtg gtctgtggcc acgagcggtg agtctcggtg aacacgtcga tgttggcag
//
LOCUS       DASYLX010001131         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2064199,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001131 DASYLX010000000
VERSION     DASYLX010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064199"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1497)
                     /locus_tag="VGP27_26995"
     CDS             complement(85..1497)
                     /locus_tag="VGP27_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020170498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEV7854980.1"
                     /translation="MITSTTGVSSGRFLTKLTVIATLGGLLFGYDTGVISGALLYMKD
                     DLQLSSFWEATVVSSLLFPGAAFGALFGGRVADRIGRKRSLLVCAGLFLVGALGCALA
                     PNVAIMVTARIVLGLGVGAAAVTCPLYLAEMAPAERRGRMVTINELMIVTGQMLAFAM
                     NALLDHLIQDPHVWRIMLAVALIPAIALFLGMLALPDSPRWYGFKGRMAEARTVLGLS
                     RSPGEADAEFALIVDHTEHMLKAKSTPFTVVRDVPWIRRVVLIGCGLAIVQQATGINT
                     VNYYAPTILEQSGLGVSAALVATIAVGVTSVVTTIIGIVLLGYIGRRTMLSIGFAGVA
                     LSQLALSLAFLLPESTTRSYVILACMVLFVAFVQMFIGTCVWLLLSEIFPLSIRGFAM
                     GIAVFVLWCTNAIISFLFPLLNSALGSTGTFLLFVAINVGSWLFVRRYVPETKGTTLE
                     ELEERFEAQDGKGLAVSAAA"
     gene            complement(1527..>1823)
                     /locus_tag="VGP27_27000"
     CDS             complement(1527..>1823)
                     /locus_tag="VGP27_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7854981.1"
                     /translation="YGVLDDRFTRTAAKDNPTSLGGRHTVNRWPKESSWDFVAVGRGH
                     DVQFWSEFLAALNRVDTGMAVNIEHEDTELGQLEGLEVAAETLRAASQLLAQPV"
BASE COUNT          369 a          606 c          558 g          290 t
ORIGIN      
        1 cggtcggcat ggaggcctgg gcgtccgccg acagtgaagc cgccctgttg gcgttcaggg
       61 cggctttcac ctaggtacag aacctcaggc ggcagcgctc accgccagcc ctttgccgtc
      121 ctgcgcctcg aatcgctctt ccagttcctc gagtgtggtg cctttcgtct ccggcacgta
      181 tcgacgcacg aacagccacg acccgacgtt gatcgcgacg aacaggagga aggtgccagt
      241 cgagccgagc gccgaattca gcagcgggaa aagaaacgaa atgatcgcgt tggtgcacca
      301 cagcacgaac accgcaatac ccatcgcgaa gccgcggatg ctcagcggga agatctcgga
      361 caacagcagc cacacgcacg tcccgatgaa catctgcacg aacgcgacga agagcaccat
      421 acaggcgaga atcacatagc tgcgcgttgt cgactcaggc agcaggaagg ccagagacaa
      481 cgctaactgg gacaacgcaa ccccggcgaa accaatcgac agcatggtcc gcctaccgat
      541 gtagccgagc agaacgatgc cgatgatcgt cgtcacgacc gatgtcacgc cgacggcgat
      601 cgtggcgacc agcgccgcac tcaccccgag accgctctgc tccaggatcg ttggcgcgta
      661 atagttgacg gtgttgattc cggtggcctg ctgaacgatc gccaggccgc aaccgatcag
      721 caccacccgc ctgatccaag gcacgtcgcg aaccacggtg aacggggtgc tcttcgcctt
      781 cagcatgtgc tcggtgtgat cgacgatcaa cgcgaattcg gcgtccgctt cccccggcga
      841 acggctcagg ccgagtacgg tgcgggcctc cgccatccgc cctttgaagc cataccagcg
      901 cggcgaatcg ggcagtgcga gcatgccgag gaaaagggca atcgccggaa tgagcgcaac
      961 cgccaacatg attcgccaca cgtgcggatc ctggatcaga tggtcgagca gtgcgttcat
     1021 tgcgaaggcc agcatttggc cggtgacgat catcagctcg ttgatcgtga ccatccgacc
     1081 acgacgctcg gccggtgcca tctcggccag gtataacggg cacgtcactg ccgccgctcc
     1141 cacgcctaac cccaggacga tgcgcgccgt caccatgatc gccacgttcg gcgcaagcgc
     1201 acagccgagc gcaccgacca ggaacaggcc tgcacacacg agcaggctgc gcttacggcc
     1261 gatccggtcg gccacccggc cgccgaacag cgccccgaag gccgccccgg ggaacagcag
     1321 cgagctgacc acggtcgcct cccagaacga actcagctgg aggtcgtcct tcatgtacag
     1381 cagggcgccg gagatgaccc cggtgtcgta gccgaacagc aggccgccca gggtggcgat
     1441 cacggtgagc ttggtgagga accgcccgga cgatacgccc gtcgtcgaag taatcatgcc
     1501 tttgctttct ggttttcgga ttccggtcag actggctggg ccaacagttg gcttgcagcg
     1561 cgcagagttt cggcggccac ctcgaggcct tcgagttgcc cgagttcggt gtcctcgtgc
     1621 tcgatgttga ccgccatgcc ggtatcgacg cggttgagcg ccgcgagaaa ttccgaccag
     1681 aactggacgt cgtggccgcg gccgaccgcg acgaagtccc agctcgattc cttcggccac
     1741 cggttgacgg tgtgcctgcc gcccaaggac gtcgggttgt ccttcgcggc cgtcctggtg
     1801 aaccggtcat cgagcacgcc gta
//
LOCUS       DASYLX010001132         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_199442,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001132 DASYLX010000000
VERSION     DASYLX010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199442"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGP27_27005"
     CDS             <1..831
                     /locus_tag="VGP27_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7854982.1"
                     /translation="GAHIGHGGKVVGDCVECPFHGWRWGPDGTNRYIPYQPDRPNKAL
                     RLRVYPVREQYGCVFAWHQPDGKEPQWEMPDLFGKFPQFPTDPDAYYRPYPEFSSRTE
                     REPVHPQIVAENGPDSAHFHYVHGATVTPVCLDWEAVGQEWRFLTGWPDARSDDPDKM
                     ALYIHSHFSGLGFPISAFEGSSNHRLIFACTPAEDGYSDMFYSIWWPRLPGDTSDVPP
                     DSVRAKVERQFLGTVWEDLDIWRYQEYVEHPALSTVDAKPYMAMRKWATQFYEVPASV
                     "
     gene            828..1442
                     /locus_tag="VGP27_27010"
     CDS             828..1442
                     /locus_tag="VGP27_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV7854983.1"
                     /translation="MTVDLAEIASPGHTAIVTQELQGAVVGPNAGLAALAKAAQRQAL
                     PNIARLLPAARDAGVHIVHCLVQRRPDGLGANHNAPIFMMGTKRVDISPGSEGATLLP
                     EFGPAADDVVLRRWHGVGPMGGTDLDAVLRNLGVSTIVAVGVSVNIAITNLVMDAVNA
                     GYHVVVPRDAVAGIPADYADAIIDRTISLLATVTTISDLMAAWA"
     gene            1524..3182
                     /locus_tag="VGP27_27015"
     CDS             1524..3182
                     /locus_tag="VGP27_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7854984.1"
                     /translation="MTAPSPQFTVPAAAEAVVAAIGDREFIIQGDRRYTYAQIVERSN
                     RLAGYLHSRGLGCHTERSELAGHEVGQDLLGLYAYNGPEYVEGMLGAWRARVAPFNVN
                     YRYVKNELHYLLADSGATALLYHAAFAHRVADVLPDLPKLRVLIQIADDSGNDLVHGA
                     VDYESIVASRKLGLPPVEPSPDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRNMYT
                     GEVVASYDEIAKRVAENPGTKIFTLPPLMHGAAQWAVMTALTTGQTVVFPATQGKFDA
                     DEVVATVEREKILTVQVVGDAMVRPIADAIERGTADVSSLAVVANGGALLTPTAKQRL
                     IDIKPGLIVMDGVGSSETGIQMSHMSTPGAVSTGRFNAGPDTSVAAEDLASILKPGHD
                     GMGWLAQRGFVPLGYKGDAAKTAKTFPVIDGMRYSIPGDRARHLAEGGIELLGRDSVT
                     INSGGEKIFAEEVESAVLSHPAVADVVVSGRPSERWGQEVVAIVALLEGANADAEELI
                     EHAAGVIARYKLPKAVVFRPTIERSPAGKADYRWAREQALSEGA"
BASE COUNT          616 a         1196 c         1097 g          543 t
ORIGIN      
        1 ggcgctcaca tcggacacgg cggcaaggtc gtcggcgact gcgtcgaatg ccccttccac
       61 ggctggcggt ggggacccga cggaacgaac cgctacatcc cgtatcagcc cgaccgcccc
      121 aacaaggcgc tccgactgcg cgtctacccg gtgcgcgagc agtacggttg cgtgttcgcc
      181 tggcaccagc ccgacggcaa ggaaccgcag tgggaaatgc cggacctttt cggtaagttc
      241 ccgcagttcc cgaccgatcc cgacgcgtat taccgtccgt atcccgagtt ttcgagccgc
      301 accgagcgcg agcccgtgca tccgcagatc gtcgcggaga acggtccgga cagcgcgcac
      361 ttccattacg tgcatggcgc gaccgtgaca ccggtgtgcc tcgattggga ggccgtcggc
      421 caggaatggc gcttcctcac cggctggccc gatgcccgca gcgacgaccc ggacaagatg
      481 gcgctctaca tccacagcca cttctccggg ctcggcttcc cgatcagcgc attcgaggga
      541 tcgtcgaacc accggctgat cttcgcctgc actcccgccg aggacggata ctcggacatg
      601 ttctattcca tctggtggcc gcgtctgccc ggcgacacct ccgacgtgcc cccggactcg
      661 gtgcgcgcga aggtcgagcg ccagttcctc ggcacggtct gggaggacct cgacatctgg
      721 cgctatcagg agtacgtcga acacccggcg ctgtccacag tggacgcgaa accctatatg
      781 gccatgcgta aatgggccac gcagttttac gaagttcccg catcggtatg accgtcgatc
      841 tcgccgagat cgcgtcaccc gggcacaccg cgatcgtcac gcaggaacta cagggcgccg
      901 tcgtcgggcc caacgccggc ctcgccgcac tcgccaaggc cgcacagagg caggcgctgc
      961 ccaacatcgc acgactgctt ccggccgcgc gcgacgcagg agtgcacatt gtgcattgcc
     1021 ttgtgcagcg gcggccggac ggcctcggcg ccaaccacaa tgcaccgatc ttcatgatgg
     1081 gaacaaagcg agtcgatatc agtcccggca gtgagggcgc gacgctgctg cccgaattcg
     1141 gccctgccgc agacgatgtc gtactacgca gatggcacgg cgttggcccg atgggcggca
     1201 cggacctcga cgcggtgctg cgcaacctcg gcgtatccac gatcgtggcc gtcggcgtgt
     1261 cggtgaacat cgcgatcacc aacctcgtca tggacgcggt gaacgccggc taccacgtcg
     1321 tcgtcccgcg cgacgccgtg gcgggcatcc cggccgacta cgccgacgcg atcatcgaca
     1381 gaaccatctc gctgttggcg acggtcacca ccatttccga tctgatggcg gcctgggcat
     1441 gaccgacacc ggccacggca ccgtactatc cgaagcaagc ggcctgatgg tcaaattgtt
     1501 gccgcaccaa ccctgaggac ctgatgactg ccccctcgcc gcaattcacc gtgcccgccg
     1561 ccgcggaggc cgtcgtggcc gcgatcggcg accgggagtt catcatccag ggcgaccgcc
     1621 gctacaccta tgcccagatc gtcgagcgat ccaaccgcct cgcgggctac ctccactcgc
     1681 gcgggcttgg ctgccacacc gagcgttccg agctggcagg ccacgaggta ggccaggacc
     1741 tgctgggcct ctacgcgtac aacggccccg agtacgtcga agggatgctc ggcgcgtggc
     1801 gtgcgcgagt ggccccgttc aacgtcaact accgctatgt gaagaacgag ctgcactacc
     1861 tgctcgcgga ctcgggcgcc accgcgctgc tgtaccacgc ggcgttcgcg cacagagtcg
     1921 ccgacgtgct ccccgacctg ccgaagctgc gggtgctcat ccagatcgcc gacgactcgg
     1981 gcaatgacct cgtccacggc gccgtcgact acgagtcgat cgtcgcgtcg cgcaaactgg
     2041 gtctgccacc cgtcgagccg tcgcccgacg acctgtacgt cctctacacc ggcggcacga
     2101 ccggcatgcc gaagggcgtg ctgtggcgtc agcacgacat cttcatgacc tcgttcggcg
     2161 gccggaacat gtacaccggc gaggttgtcg cctcctacga cgagatcgcc aagcgcgttg
     2221 cggagaatcc cggcaccaag atcttcaccc tgccgccact gatgcacggc gccgcgcagt
     2281 gggccgtcat gaccgcgctc acgaccgggc agacggtggt attcccggcc acccaaggca
     2341 aattcgacgc cgacgaggtg gtggcgacgg tggaacggga gaagatcctg acggtgcagg
     2401 tcgtcggtga tgcgatggtc cggcccattg ccgacgccat cgaacggggt accgcggatg
     2461 tgtcatcgct ggcggtcgtc gccaacggcg gggccctgct gactccgacc gccaaacagc
     2521 gcctcatcga catcaagccg ggattgatcg tgatggacgg cgtcggatcg tcggaaaccg
     2581 gcatccagat gagccacatg tccacgccgg gtgcggtgtc gaccggcagg ttcaatgccg
     2641 gtcccgacac cagcgtcgca gccgaggacc tcgcctcgat tctgaaaccc ggccatgacg
     2701 gcatgggctg gctcgcgcag cggggcttcg tcccgctcgg ctacaagggc gatgcggcca
     2761 agacggccaa gacgttcccg gtcatcgacg gcatgcggta ctcgataccc ggcgaccgcg
     2821 cacgtcatct agccgagggc gggatcgaac tgctcggccg cgattcggtg accatcaact
     2881 ccggcggcga gaagatcttc gccgaggagg tcgaaagcgc ggtgctgtcg cacccggcgg
     2941 tggccgacgt cgtggtgtcg gggcggccca gcgagcgatg gggccaggag gtcgtcgcga
     3001 tcgtcgccct gctcgagggc gccaacgcag acgcggagga actcatcgag cacgccgccg
     3061 gtgtcatcgc ccgctacaag ctgcccaagg ccgtggtgtt ccggccgacc atcgaacgca
     3121 gcccggcggg caaggccgac taccggtggg cgcgcgagca ggcgctcagc gaaggcgcct
     3181 gagagccgat tcaccggcga caacaccggc aacgtgtcgt tcggtactcc tatactgtaa
     3241 tctttacagt acctattgca gtccgtcgaa gatgctggag gccgccacgt ggatacgccg
     3301 gtgctcgacg tcacggccga cgaagcggcc gaccttcttc gcaaccccta tcccatcttc
     3361 gagcgcaggc gacgcgaaac cagcggtgtg ttctgcggca gcgtcatgga ctggacgaag
     3421 gcttccgaag acttccggcc cgagaacctc ta
//
LOCUS       DASYLX010001133         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3600087,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001133 DASYLX010000000
VERSION     DASYLX010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600087"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VGP27_27020"
     CDS             complement(<1..958)
                     /locus_tag="VGP27_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEV7854985.1"
                     /translation="MSWPRMTASEPRGGARVNRRQNWRSAGVLFACVALSYLAGAVFS
                     WQSFGAGIDPAFFPPAGVTVAAMLLTPRSRWAVIVAAIVVVELAVDLHYGVGVGAASG
                     FALANSIEPVIGASLVWAWCNGAPDLRVRADLAKFVAAACLAGPLVGGVIGGAVSAAS
                     NDTSWPIAVAQWWAGDGIGALVVGSPILLWPKQSHILRGRLADTFLVVAATVAMSLTT
                     SWWQAPPSLLLLPVMAWAAFRLDVIGAAVAGAVLAFIVNYMTGSERGLSAEMNLAAPV
                     RLAMTQAFIAVIVLVAMLIAQEAAGRVAAIRQREAERRERDRL"
     gene            complement(1304..1957)
                     /locus_tag="VGP27_27025"
     CDS             complement(1304..1957)
                     /locus_tag="VGP27_27025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7854986.1"
                     /translation="MTPRRLRVPGQVSRPATWGDRPPVDDDEARRRLLDAAEVCYERR
                     GVSRTTVDDIAKEASVHRTTVYRYFGTRDDVLAFVLLRETAGVIDSAEQAMLRDGPFG
                     DRLLDALDGAVGAVQESRWLSVLFSPESLTMTVSVAASGAFRDRIRDVLRPHVEAAKA
                     GGELREQLDPDAVADWLVRVAQMLLMEHLTTRPDETRPDRRALLRDFVIPGILNSTQ"
     gene            complement(2057..>2431)
                     /locus_tag="VGP27_27030"
     CDS             complement(2057..>2431)
                     /locus_tag="VGP27_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7854987.1"
                     /translation="RACPEAATWQGIDIPALTPMIFSINAANRDPAVYPRPDDFDITR
                     NVMPTVSFGQGRHSCIGNWLANTELVAALRALIERLPDLALDPRYAETSSITSQVGTT
                     LRGPNALRVMFTPSRSRPAAAV"
BASE COUNT          424 a          819 c          821 g          367 t
ORIGIN      
        1 gcagccggtc gcgttcacga cgttcggctt cccgctgtct gattgccgcg acgcgtccgg
       61 cggcttcttg cgcgatcagc atggcgacca gcacgatgac cgcgatgaat gcctgtgtca
      121 tcgccagccg taccggcgcg gccaggttca tttccgcgga caagccgcgc tccgagcctg
      181 tcatgtagtt gacgatgaag gccaataccg ccccggcgac ggccgcaccg atcacgtcga
      241 gccgaaacgc cgcccacgcc atcaccggca gcagcagcaa gctcggtggc gcctgccacc
      301 aggatgtcgt cagcgacatc gcaacggtgg cggcgaccac cagaaaagta tccgcaaggc
      361 ggccccgcag tatgtgagac tgtttgggcc acaataaaat tggtgagccc actacgagag
      421 cgccgatgcc gtcgcccgcc caccactgcg caacggcaat gggccatgaa gtgtcgttgg
      481 acgcggcact gaccgcaccg ccgatcaccc ccccgaccag tggaccagcc aggcaagccg
      541 ccgcgacgaa tttggccagg tccgcccgaa cccgtaggtc aggcgcgccg ttgcaccagg
      601 cccagaccag cgatgctccg atcacaggct cgatagagtt ggccaacgcg aatcccgacg
      661 cagcgcctac gccgacgccg taatggagat cgacggccaa ctccaccacg actatcgcgg
      721 cgacgatgac cgcccatcgc gatcgcggtg tcagcagcat cgcggccacg gtgacgccgg
      781 cgggcgggaa gaacgccgga tcgatcccgg cgccgaagga ttgccaagaa aacaccgccc
      841 cggccagata ggacagcgcc acgcaggcga acagcacacc cgccgaccgc cagttctgac
      901 gccgattcac gcgcgcgccg ccgcgcggct cactcgccgt catccggggc caagacaccg
      961 tgatgagcct atcgactgcg ttgtcactag ggcgcgttac tttcattcaa ggacaagcga
     1021 atgaaccggt atgtcgactg cgggcagctc accgcggtcg acagggtggc aaccatccgc
     1081 cctggcgctt cagtgctatt cgaggccaca ctgtccccgc caccccggtg gcggggggca
     1141 cgaggtggcg atgaaggccc gtctacccat cgagggcgtc tctacgcgcg tagtttctcg
     1201 cgccctctcc gggaatcgag acggcaaggg ggttttggcg ctgccggacg tctcggtggt
     1261 ggtgccgggc tgaaacagcg cggctttcgt gaacgcgctg acatcattgg gtcgagttga
     1321 gaatgccggg gatgacgaag tcgcgaagca acgcgcggcg gtcgggccgg gtctcgtcgg
     1381 gccgcgtggt gagatgttcc atcagcagca tctgcgccac ccgcaccagc cagtcggcga
     1441 cggcgtcggg gtcgagttgt tcccgcagtt cgccgccggc cttcgccgcc tcgacgtgcg
     1501 gtcgcaacac atcgcggatc cggtcgcgga acgcaccgga cgccgcgacg gagaccgtca
     1561 tggtcaggct ttcgggggag aacagcacgc tgagccaacg ggactcttgg acggctccga
     1621 ccgcgccgtc cagcgcgtcg agcaggcggt caccaaaggg cccgtctcgc agcatggcct
     1681 gttcggcgct gtcgatgact ccggctgtct cgcgcagaag tacgaaagcc aagacgtcgt
     1741 cgcgcgttcc gaaatagcga tacaccgtgg tgcggtgcac cgaggcctcc ttggcgatgt
     1801 catcgacagt ggtgcgactg acgccgcgcc gctcgtagca gacctcggcc gcgtccagca
     1861 ggcgccggcg tgcttcgtcg tcgtcgaccg gtggccggtc accccaggtt gccgggcgcg
     1921 agacttgccc agggacgcga agtcggcgcg gtgtcacggt cttctccgtc ccggtggggc
     1981 caccctgagg acaacctagc gaacgctttg cggcgtatgg cgtcattcgg cggctgaacc
     2041 cgggccggcg gcgcgcctag accgcggcgg ccggccggct ccgcgacggc gtgaacatca
     2101 cgcgcagcgc attgggccca cgcagcgtgg tgccgacctg gcttgtgatg gaggaagttt
     2161 cggcgtaccg gggatcgagc gcgaggtcgg gtaaccgctc gatcagggcc cgcagcgccg
     2221 cgacaagttc ggtgttggcc agccagttcc cgatgcagct gtggcggccc tgcccgaacg
     2281 agacggtcgg catcacattg cgggtgatgt cgaagtcgtc gggccgcggg tacaccgccg
     2341 gatcgcggtt ggcggcgttg atcgagaaga tcatcggggt cagagcgggg atgtcgatcc
     2401 cctgccacgt cgcggcctcg gggcaggcgc g
//
LOCUS       DASYLX010001134         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3600141,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001134 DASYLX010000000
VERSION     DASYLX010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600141"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1580)
                     /locus_tag="VGP27_27035"
     CDS             complement(<1..>1580)
                     /locus_tag="VGP27_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7854988.1"
                     /translation="QRQRAAQLLDVARKYPDFPIVLETIRECLQDVYDVPTLTEVMNK
                     IAQRRLRIVEVETQTPSPFAASLLFGYVGAFMYEGDSPLAERRAAALSLDSMLLAQLL
                     GRVELRELLDPQVIASTAAQLQHLSEDRAARDAEGVADLLRLLGPLTEAEIAERCTAE
                     DVGGWLEGLRETKRALTVSYAGQTWWVAIEDIGLLRDGVGVAVPVGVPAIFTEGVADP
                     LGELLGRYARTRAPFTTHDAATRFGLGQRVAADVLGRMANDGKLVRGEFTDSAEAGDQ
                     WCDASVLKILRRRSLAALRAQVEPVSTTAYAKFLPAWQQVGAERVSGIDGLASVIDQL
                     AGVPIPASAIEPLVFSTRVRDYQPAMLDELLASGEVTWSGAGTIGNGDGWIAFHSGET
                     APLTLAQPTEIDFTDTHRAIMEVLGGGGAYFFRQLCGPDTEQFKQALWELIWAGWITG
                     DTFAPVRALLGGTGKRSGRPAHRQAKRPPRLSRYSVAHAQTRASDPTVAGRWSALPAA
                     ELDSTVRAHFQAELLLNR"
BASE COUNT          254 a          566 c          514 g          246 t
ORIGIN      
        1 tgccggttga gcagcaactc ggcctggaag tgggctcgca cagtcgaatc cagctcggcg
       61 gccggaagcg cggaccagcg tcccgcaacg gtgggatcgg atgcgcgtgt ctgtgcgtgg
      121 gcgacgctgt agcggctcag ccgcggcggc cgcttcgcct ggcggtgcgc gggccgaccc
      181 gatcgcttgc cggtgccgcc gagaagcgcc cgcacgggcg cgaaggtgtc accggtgatc
      241 catccggccc aaatcagttc ccacagagcc tgtttgaact gctcggtgtc gggcccgcag
      301 agctgacgga agaaatacgc accgccgccc ccgagaacct ccatgattgc gcggtgggtg
      361 tcggtgaaat cgatctcggt cggctgggcc agcgttagtg gcgcggtttc gccggagtgg
      421 aacgctatcc agccgtcacc gttaccgatc gtgcccgccc ctgaccacgt cacctcgccc
      481 gaggccagca attcgtcgag catcgccggc tggtagtcgc gaacgcgcgt agagaacacc
      541 aatggctcga tcgccgatgc cggtatcggc acccctgcca gttgatcgat cacagacgcg
      601 agaccgtcga tcccggagac gcgctccgca cccacctgct gccaggcggg aaggaacttg
      661 gcgtatgcgg tcgtactgac cggttcgacc tgcgcccgca gcgccgccaa cgaccgccgc
      721 cgcaggatct tcagcacgct cgcgtcgcac cactggtcgc ctgcctctgc cgagtcggtg
      781 aactctccgc gcaccagctt gccgtcattg gccatccggc ccaacacatc tgccgcgaca
      841 cgctgcccga gtccgaaccg agtcgctgca tcatgcgtgg tgaacggggc gcgggtgcgg
      901 gcgtaccggc cgagcagttc gccgagcgga tcggcaacgc cttcggtgaa gatcgcgggc
      961 acgccgaccg gcaccgccac accgacgccg tcgcgcagca ggccgatgtc ctcgatcgcc
     1021 acccaccagg tctgcccggc atatgacacc gtcagcgcgc gctttgtctc gcgcagccct
     1081 tccagccagc ccccgacatc ctcggcggtg caacgctccg cgatctcggc ctccgtcagc
     1141 ggccccagca ggcgcagcag gtcggcgaca ccttcggcgt cgcgggcggc ccggtcctcg
     1201 ctcaagtgct gcagctgcgc ggcggtgctg gcgatcacct gcgggtcgag cagttcgcgc
     1261 agctcgacgc ggcccaacag ctgggccaac agcatgctgt ccagcgacag cgccgcggca
     1321 cggcgctcgg ccagtgggct gtcgccttcg tacatgaacg caccgacgta accgaacagc
     1381 agcgacgccg cgaacggtga cggtgtctgg gtttccacct cgacgatccg cagccggcgc
     1441 tgcgcgatct tgttcatcac ctcggtcaac gtcggcacgt cgtagacgtc ctgcaggcac
     1501 tcgcggatcg tctccagcac gatcgggaag tcgggatact tacgggccac gtcgagcagc
     1561 tgtgccgcac gctgtcgttg
//
LOCUS       DASYLX010001135         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3928540,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001135 DASYLX010000000
VERSION     DASYLX010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928540"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="VGP27_27040"
     CDS             <1..1358
                     /locus_tag="VGP27_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV7854989.1"
                     /translation="VSQFSLAVPWEALADHTVTDEPVVRGVSTRWYVSSVELGQGVAD
                     TAPLSTDPTFLGRVQPYSVCLPSTYAPGRKLPLTLLLHSLALGQNQFAAIGPRLLHQI
                     CEDRDSVVVTPLARGPSCWYLDEGELDVWEVWARVAEQLGTDPNRTVISGYSMGGYGT
                     YKLALAYPEVFAQAVVLAGPPSCGVRLLPDVDIPANLDPHSHCAREGDTLRLLTNARW
                     LPFVIAHGLLDELVPFTSVLFQIHELDRLAYRYRFSVYPTEDHIGWVFQDEFDDPVSH
                     MGTGLRQGDPGHITFTWFPQLVRPDLGIGPHQVWWLSDLTADPDTTATRGARASVDAR
                     SHARPDPTRTTQRRRGFEPDLDLSPGLYTELIWSIGPAVAPLPSLALNLSRVAGLTID
                     VARAGLAALSASTIAVVTDTPAHITLAALPPDTTVLLDGRPTGPTVAVPVGQHTITLT
                     H"
     gene            1452..>1678
                     /locus_tag="VGP27_27045"
     CDS             1452..>1678
                     /locus_tag="VGP27_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7854990.1"
                     /translation="MPMTNPMRHPFRVPRVEKPRAEGRFYLPTGRRLGFAEFGDPSGE
                     PVLWFHGTPGGRRQFPLIGRRAAEKLGLRVVS"
BASE COUNT          235 a          617 c          535 g          291 t
ORIGIN      
        1 acgtgtccca gttctcgctg gccgtcccat gggaggcgct cgccgatcac accgtcaccg
       61 acgagcccgt cgtcaggggc gtctccaccc ggtggtacgt ctcgtcggtc gagctgggac
      121 agggcgtcgc cgacaccgcg ccgttgagca ccgaccccac cttcctcggc cgggtgcagc
      181 cctattcggt gtgccttccc tcgacgtacg cgccgggccg taagttgccg ctgacgctgc
      241 tgctgcattc acttgcgctg ggccagaacc agttcgccgc catcggtccg cggctgctgc
      301 accagatctg cgaggaccgg gactccgtgg tggtgacacc gctggcccgc ggtccctcgt
      361 gctggtatct cgacgagggc gagctcgacg tgtgggaggt ctgggcgcgg gtggccgagc
      421 agttgggcac cgaccccaac cgcaccgtga tctccggcta ctcgatgggc ggctacggta
      481 cctacaaact ggcgctggcc tatccggagg tgttcgccca ggcggtcgtg ctggccggcc
      541 cgccttcgtg tggcgttcga ctgctgcccg acgtcgacat cccggcaaac ctggacccgc
      601 actcgcactg cgcgcgcgag ggcgacacct tgcggctgct caccaacgcg cggtggcttc
      661 cgttcgtcat cgcccacggg ctgctcgacg agctggtgcc gttcacctcg gtactgttcc
      721 agatccacga gctcgaccgg ctcgcctacc gctaccggtt cagcgtctat ccgaccgagg
      781 accacatcgg gtgggtcttc caggacgagt tcgacgatcc ggtttcgcac atgggcaccg
      841 gcctccggca gggcgacccc ggccacatca cgttcacgtg gtttccgcag ttggtgcggc
      901 ccgacctcgg catcgggccg caccaggtct ggtggctgtc ggacctgacg gccgaccccg
      961 acaccacagc cacgcggggc gcgagagcct cggtcgacgc gaggtctcat gcgcggccgg
     1021 accctacgcg caccacgcag cgtcgtcgtg gcttcgaacc ggacctcgac ctctcacccg
     1081 gcctgtacac cgaactcatc tggagcatcg ggcctgctgt cgctccgttg ccttccctgg
     1141 ccttgaatct cagccgcgtc gcagggctca ccatcgacgt cgccagggcc gggcttgccg
     1201 cgttgtcggc atcgaccatc gccgtggtga ccgacacgcc ggcgcacatc acgctggccg
     1261 cgctgccacc cgacacgacg gtcctgctcg acggtcggcc gaccggtccg acggtggcgg
     1321 tgcccgtcgg tcaacacacc atcacgttga cccattgagg cgtcgagtca ccgtctttcg
     1381 cgtaaagcgt ccccggaatg gccgacacgc tgtgagcatt gcacgatgac ggatcatcac
     1441 ccccgatgaa gatgccgatg accaacccga tgcggcaccc cttccgcgtt ccacgcgtcg
     1501 agaagccgcg cgccgaaggt cgcttctacc tgcccaccgg ccgtcgtctg ggcttcgccg
     1561 agttcggcga cccgtcgggg gagccggtgc tgtggttcca cggcacaccc ggcggcaggc
     1621 gccaatttcc gctgatcggc cgtcgcgccg ccgagaagct cggcctgcgt gtggtctc
//
LOCUS       DASYLX010001136         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2943580,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001136 DASYLX010000000
VERSION     DASYLX010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943580"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VGP27_27050"
     CDS             complement(<1..106)
                     /locus_tag="VGP27_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEV7854991.1"
                     /translation="MKILMVSWEYPPVVVGGLGRHVYQLATALAAAGHE"
     gene            complement(240..>1583)
                     /locus_tag="VGP27_27055"
     CDS             complement(240..>1583)
                     /locus_tag="VGP27_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEV7854992.1"
                     /translation="GMTPVVTAQLDDPYCLTGMHHWLANWRLRAQEATTLPEPLRQFG
                     VREVAEADRELDEFTTLWQHGGSPLLRELIDTNTIELLGGPLSHPFQPLLNSRLREFA
                     LREGLADAHQRLAHTPTGIWAPECAYAPGMEFDYAAAGVGHFMVDGPSLHGDTALGRP
                     VGDSDVIAFGRDLQVSYRVWSPKSGYPGHAAYRDFHTYDHVTGLKPARVTGRNVPSEA
                     KAPYDPERADRAVDGHVADFVEVVRQRLVTESERIGRPAHVIAAFDTELFGHWWYEGP
                     LWLERVLRALPAAGIRVGTLSDAVADGFTGSAVELPPSSWGSGKDWSVWSGEKVADIV
                     QLNTEVVDTALSTVDKAFEQNASVGAPVARDVVADQILRETLLTVSSDWPFMVSKDSA
                     ADYARYRAHLHAHATREIAGALATGRREQAERLAAGWNRADGLFGALDARRLPRS"
BASE COUNT          241 a          552 c          553 g          237 t
ORIGIN      
        1 tctcgtgtcc ggccgcggcc agcgccgtcg ccagctgata gacatgccgt ccgagcccgc
       61 cgaccaccac cggcgggtac tcccacgaca ccatcaagat cttcacggtc gctcgatccc
      121 gcgcgagcgt gcgcgaagtg ctgctctgca ccggcgtgtc ggggagcaga cacgcacggt
      181 cgcggggcaa gagctccccg ttgcggggca agaggtcccc cttgcggggc gaggcggggt
      241 catgagcggg ggagccttct ggcgtcgagg gcgccgaaca gcccgtccgc gcggttccag
      301 ccggccgcca accgctcggc ctgctcgcgg cggcccgtcg cgagcgctcc ggctatctcg
      361 cgggtggcat gcgcgtgcag atgggcgcgg taacgggcgt agtcggccgc ggaatccttg
      421 ctgaccatga acggccagtc gctcgacacg gtcagcagcg tctcacgcag gatctggtcg
      481 gcgacgacgt cacgggcgac cggggcgccc accgacgcgt tctgctcgaa cgccttgtcc
      541 actgtgctga gtgcggtgtc cacgacctcg gtgttgagct gcacgatgtc ggcgaccttc
      601 tcgcccgacc acaccgacca gtccttgccg gaaccccaag agctgggcgg caattcgacg
      661 gcgctcccgg tgaagccgtc ggcgaccgca tcggacagcg tacccacccg gatgcccgcg
      721 gcgggcaacg cccgcaacac ccgttccagc cacagcggcc cttcgtacca ccagtggccg
      781 aacagttcgg tgtcgaacgc ggcgatcacg tgtgcgggcc gaccgatccg ctcgctctcc
      841 gtgacgagcc gttggcgcac cacctcgacg aagtcggcga catgtccgtc gaccgcccga
      901 tcggctcgct cggggtcgta gggcgccttg gcctccgaag gaacgttgcg gccggtgacg
      961 cgggccggct tgaggccggt gacgtgatcg taggtgtgga agtcgcggta ggcggcgtgg
     1021 ccgggatagc cggacttcgg cgaccacacc cggtagctga cctgcagatc gcgaccgaac
     1081 gcgatgacgt cggagtctcc gaccggacgc ccaagcgccg tgtcgccgtg cagcgacggg
     1141 ccgtccacca tgaaatgccc gacaccggct gcggcgtagt cgaactccat gccgggggcg
     1201 tacgcgcatt ccggcgccca gattccggtc ggcgtgtgcg ccaaccgctg gtgggcatcg
     1261 gcaaggcctt cgcgcagcgc gaactcccgc agccgcgaat tcagcagcgg ctggaacgga
     1321 tgcgacagcg ggccgcccag cagttcgatg gtgttggtgt cgatcagctc acgcagcagc
     1381 gggctgccgc cgtgctgcca cagcgtggtg aactcgtcga gctcgcggtc ggcttcggca
     1441 acctcgcgaa cgccgaactg ccgcaacggt tccggcagcg tggtggcctc ctgggcgcgt
     1501 agccgccagt tggccagcca gtgatgcatg cccgtcaggc agtacgggtc gtccagttgc
     1561 gcggtgacaa cgggtgtcat gcc
//
LOCUS       DASYLX010001137         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3271731,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001137 DASYLX010000000
VERSION     DASYLX010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3271731"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VGP27_27060"
     CDS             <1..1426
                     /locus_tag="VGP27_27060"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV7854993.1"
                     /translation="AGIPVESDEHSLTVGPDGPILLQDHYLIEQMAQWNRERTPERQP
                     HAKGSGAFGRFEVTHDVSAYTKAAVFQPGTKTDMLARFSTVAGERGSPDTWRDPRGFA
                     LKFYTTEGNYDMVGNNTPIFFIRDPMKFQHFIRSQKRRADSNLRDHDMQWDFWTLSPE
                     SAHQVTYLMGDRGIPRSWRLMNGYSSHTYMWINTAGEKFWLKYHFISDQGVDFLTQDE
                     ADHLAGADSDYHMRDLYQAIDRGEYPSWTLKVQIMPFEEAKTYRFNPFDMTKVLPHGD
                     YPLIEVGKMTLDRNPTDNHTEIEQAAFEPSNLVPGIGPSPDKMLLGRMFSYPDAHRAR
                     IGANYNLLPVNAPKSPVHSYSKDGAMRFRNVSDPVYAPNSKGGPKADTVHYGEPAGWH
                     TDGEMVHVAQTLHADDDDWGQAGTMVRDVFDDAARARLVSNIVGHLLDGVTEPVLLRA
                     FEYWRNVDKDLGDRVEQGVRAGQS"
     gene            1513..2151
                     /locus_tag="VGP27_27065"
     CDS             1513..2151
                     /locus_tag="VGP27_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV7854994.1"
                     /translation="MSERIAGIDIPDSRLASEATELVRDVASPLLFNHSRRVFLFGSL
                     QGNRLGLTADPELLYVGAMFHDLGLTEKYRSTDQRFELDGADEARRFLTAHGLPEDRI
                     RLAWLGIALHTTPETPYHLEPEVALVTAGVETDVLGIGYADLDSTLIDAVVSAHPRPD
                     FKRQILQAFTNGFKDRPDTTFGTVNADVLAHFVPGFVRTDFVEVIQSNAWPE"
     gene            2290..2682
                     /locus_tag="VGP27_27070"
     CDS             2290..2682
                     /locus_tag="VGP27_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEV7854995.1"
                     /translation="MPLLYFDVIEGRKSGEIKALLDTAHAAMVDALGVPERDRYQVVN
                     SHPAGEIVALDTGLGINRSDQLVIIHVVSRRRTRAQKEKLYALLAERLKRDCGLDPAD
                     LVVSVSENDDEDWSFGFGRAQFLTGELH"
     gene            2957..>3092
                     /locus_tag="VGP27_27075"
     CDS             2957..>3092
                     /locus_tag="VGP27_27075"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7854996.1"
                     /translation="MPYRRRLKGRKIAVLAADGFEKVELVIPLRALKLAGAKVDVVSL
                     R"
BASE COUNT          583 a         1051 c          949 g          509 t
ORIGIN      
        1 tgccggtatc ccggtcgaga gcgacgagca ttcgctcacg gtcggtccgg acggtccgat
       61 ccttttgcag gatcattatc tcatcgagca gatggctcag tggaaccgcg agcgcactcc
      121 agagcgtcag ccgcatgcca agggtagcgg cgcgttcggt cgcttcgaag tgacccacga
      181 tgtcagtgcc tataccaagg ccgcggtgtt ccagccgggc accaagaccg acatgctggc
      241 ccggttctcc acggtggccg gcgagcgggg cagccccgat acctggcgtg atccccgcgg
      301 ctttgcgctg aagttctaca ccaccgaggg caactacgac atggtcggca acaatacgcc
      361 gatcttcttc atccgcgacc cgatgaagtt ccagcacttc atccgctccc agaagcgccg
      421 tgcggacagc aaccttcgcg accatgacat gcagtgggat ttctggacgc tgtcgccgga
      481 atcggcccac caggtcacgt acctgatggg tgaccggggc atcccgcgca gctggcggct
      541 aatgaacggt tattccagtc acacctacat gtggatcaac acggccggcg agaagttctg
      601 gctgaaatat cacttcattt cagaccaggg tgtcgatttc ctcacccagg atgaggccga
      661 ccacctcgcg ggcgcggaca gcgattacca catgcgcgac ctgtaccagg cgatcgaccg
      721 cggtgagtac ccgagctgga cgctcaaggt ccagatcatg ccgttcgagg aggccaagac
      781 ctaccggttc aacccgttcg acatgaccaa ggtgttgccg cacggcgact acccgctcat
      841 tgaggtcggg aagatgacgc tggaccgcaa cccgaccgac aatcacaccg agatcgagca
      901 ggcggccttc gagcccagca atctcgtgcc gggtatcggc ccgagcccgg acaagatgct
      961 gctgggccgg atgttctcct acccagatgc ccaccgtgcc cgcatcgggg cgaactacaa
     1021 cctgctgccg gtgaacgccc ctaagtcgcc cgtgcacagc tacagcaagg acggcgcgat
     1081 gcgcttccgc aacgtctccg acccggtgta cgcaccgaac tccaagggcg gcccgaaagc
     1141 cgacaccgtg cactacggcg agccggcggg ctggcacacc gacggagaga tggtgcacgt
     1201 cgcccaaacc ctgcacgccg atgacgacga ctggggccag gccggcacca tggtccgcga
     1261 cgtgtttgac gacgccgccc gcgcgcggct ggtcagcaac atcgtcgggc acctgctcga
     1321 tggcgtgacc gagcccgtac tcctgcgtgc cttcgagtac tggcgcaacg tggacaagga
     1381 cctgggcgac cgggtcgagc agggcgtccg cgccggacag tcctgaccgc gcccgtctga
     1441 ccggggcccg gcgccggagg cgccgggccc ttaccacctc agcctgcgca actaccagac
     1501 caggagacca ccatgtctga acgcatcgcc ggcatcgaca tcccggacag ccggctggcc
     1561 agcgaggcga ccgaactggt ccgcgacgtc gccagcccgc tgttgttcaa tcactcccgc
     1621 cgggtgttcc tcttcggttc gctccagggc aaccgactcg gcctgaccgc cgacccggag
     1681 ctgctctacg tcggcgcgat gttccacgac cttggcctga cggagaagta ccggagcacc
     1741 gaccagcgtt tcgagctcga cggtgcggac gaggcccgcc gctttctcac cgcgcacggc
     1801 ctgcctgagg accgtattcg tcttgcgtgg ctgggcatcg cgctgcacac cactccggaa
     1861 accccctacc acctagaacc cgaggttgct ctggtcaccg ccggcgtgga aaccgacgtg
     1921 ctcggcatcg gctacgccga cctcgacagc accctcatcg acgccgtcgt cagcgcgcac
     1981 ccgcggccgg acttcaaacg gcagatcctg caggcgttca caaacggctt caaggatcgc
     2041 ccggacacca ccttcggcac ggtcaacgcc gacgtcctcg ctcatttcgt gcccggtttc
     2101 gtccgtaccg atttcgtcga ggtcatccag agcaacgcct ggcccgaata gtcaggcctc
     2161 cacggtggtc gagatgaccc gcaccaccgc atcggccaca aaacggcgcc gcttcccgac
     2221 gcaccgctgg ccccccgtgg ggtaagcgcc gaatcgggtg ccggtgttag ggccccggaa
     2281 ggaaaagtca tgcccctgct gtacttcgat gtcatcgaag gccgaaaatc tggtgaaatc
     2341 aaagcgttgc tcgacacagc gcacgcggcc atggtggacg cgctcggagt gcccgagcgg
     2401 gaccggtacc aagtcgtgaa cagccatccc gctggcgaga tcgtcgcact cgacaccggt
     2461 ctgggcatta atcggtccga ccagctggtc atcatccacg tggtcagccg caggcgcacc
     2521 cgcgcgcaga aggagaagct ttacgcactg ctggccgagc ggttgaaacg cgactgcggt
     2581 cttgacccgg ctgatctggt cgtctcggtg tccgagaacg atgacgaaga ttggtccttc
     2641 ggatttggcc gcgctcaatt cctgaccggg gagttgcact gagcattcgc tgcactcgca
     2701 gagcagggca tccgcgggac agcccttaac tgggtcttgc cgttgcacgg tcgggcaaac
     2761 cttgccaaag aggagtaggc ccggggcgcg gcctccgact cgtccaccaa ggtgtcgggt
     2821 aggtcgggta cgggtatcca cctatcggtt cagcagccag ccaccgtgac gaacaacttc
     2881 tggcgcctcg gctgaagctt ccgccgggcg ggagtgacgc cacctctgcg gcgccatcga
     2941 gcgacgggag gacaacatgc cctacagacg ccggctcaag ggccgcaaga ttgccgtcct
     3001 ggcggccgac ggcttcgaaa aggttgagct cgtcattccg ctgcgagccc tgaagttggc
     3061 cggtgcgaag gttgacgtcg tgtctctacg gc
//
LOCUS       DASYLX010001138         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2066859,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001138 DASYLX010000000
VERSION     DASYLX010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066859"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGP27_27080"
     CDS             <1..259
                     /locus_tag="VGP27_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoin dehydrogenase"
                     /protein_id="HEV7854997.1"
                     /translation="DPDGRGRSHEQMVAEFAAITMTQPAKAAEIIHRGVEQGKARILV
                     GPDAYVIDILTRLAPTRYYDVLSVIEARLRDRAKRPAAATA"
     gene            266..1270
                     /locus_tag="VGP27_27085"
     CDS             266..1270
                     /locus_tag="VGP27_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7854998.1"
                     /translation="MRAVVITKHGDFSVLKVAERPDPPPPSAGQVRITVRAAGVNFAD
                     HLARVGLYPDAPKPPCVVGYEVSGIIAAVGDGVDPARIGERVFGGTRFGGYSELVNIA
                     AADAIPLAESLSFEQGAAIPVNYATVWAALHGYGSLRAGERVLIHAAAGGVGIAAIQL
                     AKAAGAEIHGTASPGKHARLAEFGVDRAIDYRKDGWWNGLPPYDIVLDAMGGKSLKRS
                     YDLIRPGGRIVAYGLSAMQQGEKRSLRTALPQLLPMLRGFNLVKQLSDSKAVIGLNML
                     KLWDDRGTLEPWIGPLTTALDDGVAVPVVHEAVPFANAGEAHRILAARENVGKVVLVP
                     "
     gene            complement(1267..2064)
                     /locus_tag="VGP27_27090"
     CDS             complement(1267..2064)
                     /locus_tag="VGP27_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7854999.1"
                     /translation="MKVHHLNCGTMHPVRGPECVCHVLLLETANGLILVDAGFGIDDC
                     ADPKGRVGPVRFVTRPVFESTEAAAHQLDRLGFRREDVRHIVLTHLDTDHVGGAADFP
                     HARIHVTAAEAVAAFASPTRGEKVRYGRQQWVRERDVVEHSPNGEAWRGFPAAKELDD
                     IAPSIVMVSLPGHTRGHTCIAVDAGHRWVLHCGDAFYHYGSLDGVHVPRTLWAMETAV
                     AFDRKKVWDNHARLSELYHKAEPDLCIAPAHDMKLFERAKATAEPTA"
     gene            complement(2061..>2241)
                     /locus_tag="VGP27_27095"
     CDS             complement(2061..>2241)
                     /locus_tag="VGP27_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855000.1"
                     /translation="PPTGKAFRVPIIAVFFFDGDRIVNERVYFDSASLVTQIGRGEVL
                     ALVGDAGAREEFPRR"
BASE COUNT          368 a          740 c          769 g          364 t
ORIGIN      
        1 ggacccggac gggcgcggtc gcagccacga gcagatggtg gctgagttcg ccgcgatcac
       61 catgacccag cccgccaagg ccgccgagat catccaccgc ggtgtcgagc agggcaaggc
      121 acgcattctg gtgggacccg atgcctacgt catcgacatc ctgacccggt tggcacccac
      181 gcgttactac gacgtgctca gcgtgatcga ggcgcggctg agagaccgcg ccaagcggcc
      241 tgccgccgcg acggcctgat ccggcatgcg cgccgtcgtc atcaccaagc acggtgactt
      301 ctccgtcctg aaggttgcgg agcggccgga cccgccgccg ccatcggcgg gacaggtgcg
      361 gatcacggtc cgggcggcag gcgtgaactt cgccgatcac ctggcgcgcg tcggcctcta
      421 cccggatgcg cccaagccgc cctgcgtcgt cggatacgag gtctcgggga tcatcgcggc
      481 cgtcggcgac ggcgtcgacc ccgcacgcat cggcgaacgc gtcttcggcg gaacacgatt
      541 cggcggctac agcgaactcg tgaacattgc ggcggccgac gcgatcccgc tcgctgaatc
      601 gctgagcttc gaacagggtg cggcgatacc ggtcaactac gccaccgtgt gggcggcgct
      661 gcacggctac gggtcgttgc gcgccggtga acgcgtgctc atccatgccg cagcaggcgg
      721 cgtcgggatt gcggcgatcc agttggccaa ggccgccggg gccgagatcc acggcacggc
      781 atcgccgggt aagcacgcgc gtctcgccga attcggtgtc gaccgcgcca tcgactatcg
      841 caaggacggc tggtggaatg ggttgccgcc gtacgacatc gtgctcgacg cgatgggcgg
      901 aaagtcgctg aagcgttcct acgacctgat ccggcccgga ggccgcatcg tcgcgtacgg
      961 cttgtccgcg atgcaacagg gcgagaagcg ctcgctgcgc accgcgctgc cgcagcttct
     1021 gccgatgctg cgcggtttca atctggtcaa gcagctttcc gactcgaaag ccgtcatcgg
     1081 actgaacatg ctcaagctgt gggatgaccg gggcacgctg gagccgtgga tcggcccgct
     1141 caccacggcg cttgacgacg gcgtcgccgt cccggtcgtt cacgaagccg tcccgttcgc
     1201 caacgccggt gaggcacacc ggatcctggc cgcgcgggag aacgtcggga aagtggtcct
     1261 ggtgccctaa gcagtgggct cagcggtggc tttggcgcgt tcgaacagct tcatatcgtg
     1321 tgcgggcgcg atgcagaggt ccggctccgc cttgtggtag agctctgaga gccgagcgtg
     1381 gttgtcccac accttcttcc gatcgaaggc gaccgcggtc tccatggccc agagggtacg
     1441 cggcacgtgg acgccgtcca gggagccgta gtggtagaac gcgtccccgc agtgcaggac
     1501 ccaacgatgt ccggcgtcga ccgcgatgca ggtgtgccct ctggtgtggc cgggcagcga
     1561 aaccatgacg atgctgggcg cgatgtcatc gagctccttg gccgccggaa aacctcgcca
     1621 tgcttcgccg ttcgggctgt gctcgacgac gtcgcgctct ctcacccatt gctggcggcc
     1681 gtatctgact ttctcgccgc gggtgggtga ggcgaatgcc gcgactgctt ccgcggcggt
     1741 gacatggatg cgtgcatgcg ggaagtctgc cgctcctccg acgtggtcgg tgtccaggtg
     1801 ggtcagcacg atgtgacgga cgtcctcgcg gcggaacccg agccggtcga gctggtgtgc
     1861 cgccgcctcg gtggactcga acaccgggcg ggtgacgaaa cggaccgggc ccacccgccc
     1921 tttcggatcg gcgcagtcgt cgatcccgaa accggcgtcg acgaggatca gaccgttggc
     1981 ggtctccagc aacaggacgt gacacacgca ctcgggaccg cgaacaggat gcatggtgcc
     2041 gcagttgagg tggtggacct tcatcgccta gggaactcct cgcgtgcgcc ggcatcgccg
     2101 accagcgcca atacttcacc gcggccgatc tgcgtcacca ggcttgccga atcgaagtag
     2161 acacgctcgt tgacgatgcg gtcgccgtcg aagaagaaca cggcgatgat cggcacccgg
     2221 aacgccttac ccgtcggcgg a
//
LOCUS       DASYLX010001139         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1297278,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001139 DASYLX010000000
VERSION     DASYLX010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297278"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VGP27_27100"
     CDS             <1..145
                     /locus_tag="VGP27_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV7855001.1"
                     /translation="RPVVQIPLGHDLIHAPTGAVLLQLRWLGLEGRDDPVNQLEQPVF
                     AWR"
     gene            232..420
                     /locus_tag="VGP27_27105"
     CDS             232..420
                     /locus_tag="VGP27_27105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855002.1"
                     /translation="MMTTHAAAANVVSLESHRTWRSSRRRSEEFMAAMRRHPSYQGAL
                     LESRAHRRDAVVLSLRRR"
     gene            complement(436..>2330)
                     /gene="helR"
                     /locus_tag="VGP27_27110"
     CDS             complement(436..>2330)
                     /gene="helR"
                     /locus_tag="VGP27_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase recycling motor ATPase HelR"
                     /protein_id="HEV7855003.1"
                     /translation="EYEPLLIDWRAPAARPFYTATGANPESMRRRRQFHTRGRRVIDF
                     TDEELGRPSGTGSGGGSDAALLAAVNAPRGEGMRDIVATIQAEQDEIIRLDHPGVLVI
                     EGGPGTGKTVVALHRVAYLLYTQRKRIENHGVLVVGPNPAFLHHIGRVLPSLGETDVV
                     FMTTGDLMPGLRVTDEDNPEAARLKGSLTILDVLAAAVADRQRLPEHPISIEMGDVTL
                     RIDAETVEWAIQEARASGLPHNEARAVFTEIITYVLSERAIARVGRGWLTREDRKAWE
                     LLRAKLLDELSDDATFTAAIDGLWPILTPESLLASLYSSPERLRAAGADAALFRADGD
                     AWTVSDVALLDELVDLLGPDKPADDSAERERQAEARYAANVLDSMLKREDMMDDEDHL
                     LATDMLYAEDLADRFVQHDTRELAERAAADRDWTYRHVVVDEAQELSAMDWRVLMRRC
                     PSRSFTVVGDLAQRRSVAGATSWDAMLEPYVPGRWVYRSLSVNYRTPAEIMSVAADLL
                     AEFAPGVQAPESVRATGVHPWSRRVTEIELASAIEEFVEDESGREGTSVVIGPPGVHG
                     AVPPSETKGLEFDAVLVVEPERILAEGPRGAAELYVALTRATQRLGVLHSGALPTALS
                     GLGGRS"
BASE COUNT          360 a          810 c          778 g          382 t
ORIGIN      
        1 gcgcccggtc gtgcagattc cgctcgggca cgacctcatc cacgcgccga cgggtgcggt
       61 cctgctgcaa ctgcgatggc tggggttgga gggccgtgat gaccccgtga accagctgga
      121 gcagccggtc ttcgcctgga ggtagccgga ataagtttcc ccgaccgcag gtttgcacat
      181 ctggcagccc gggaacttct cagtgaacgt attgttcagc gaggtgggta gatgatgacc
      241 acacatgccg cagccgcgaa cgtggtgtcg ctcgaatcgc atcgcacgtg gcgctcatcc
      301 cggcgacggt cagaggaatt catggccgcg atgcgccggc acccgtccta ccaaggtgcg
      361 ctgctcgaat cccgcgcgca ccgccgcgac gccgtcgtgc tgagtcttcg ccgacgctga
      421 acccgtcgcg caacgtcagc ttcgaccccc gagcccgctc agcgccgtcg gcagcgcgcc
      481 cgaatgcagc acgccgaggc gttgagtagc gcgggtcagg gcgacgtaca actcggccgc
      541 gccgcgcggg ccctcggcga ggatccgctc cggctccacc accaggaccg cgtcgaactc
      601 caggcctttg gtctccgagg gcggcaccgc cccatgcacc cccggtggac cgatcacgac
      661 gctggttcct tcacgaccgg actcatcctc aacgaactct tcgatggcag aagctaattc
      721 gatctcggtg acccgccgtg accacgggtg cacgcccgtt gcgcgcacgg attccggtgc
      781 ctgcacgccc ggagcgaact cggcgagcag atcggcggcg accgacatga tctccgcggg
      841 cgttcggtag ttcaccgaca gcgaccggta aacccagcgg ccgggtacat agggctccag
      901 catcgcgtcc cacgacgtgg ccccggcgac cgaacgccgc tgcgcgagat cgccgaccac
      961 cgtgaaggac cggctcgggc agcgccgcat cagcacccgc caatccatcg cggacagttc
     1021 ctgcgcctcg tcgaccacga cgtgccggta ggtccagtcc cggtcggctg cggcacgctc
     1081 cgcgagttcc cgggtgtcgt gctggacgaa gcgatccgcg aggtcctcgg cgtagagcat
     1141 gtccgtggcc agcaggtgat cctcgtcgtc catcatgtcc tcgcgcttga gcatggagtc
     1201 cagcacattg gcggcgtagc gcgcctcggc ctgccgttcc cgctctgcgg agtcgtctgc
     1261 aggcttgtcg gggcccagca ggtcgaccag ttcgtcgagc agagccacgt ccgacaccgt
     1321 ccaggcgtcg ccgtcggcgc ggaacaatgc agcgtcggca cccgccgccc gcaagcgttc
     1381 gggtgacgaa tacagcgatg ccaacagtga ttccggcgtc aggatcggcc agaggccgtc
     1441 gatcgcagcc gtgaaggtgg cgtcatcgga aagttcgtcg agaagcttgg ctcggaggag
     1501 ctcccatgcc ttgcggtcct cgcgggtgag ccagccgcgg ccgacgcggg ctatcgctcg
     1561 ctcgctgagc acgtaggtga tgatttccgt gaacacggcg cgggcctcgt tgtgcgggag
     1621 tccgcttgcg cgcgcctcct ggatcgccca ttcgacggtc tcggcgtcga tgcgcagggt
     1681 gacgtctccc atttcgatcg atatcggatg ctcaggaagc cgttgccggt cggccactgc
     1741 cgcggcgagc acatccagga tcgtcaacga gcccttgagc cgtgcagcct ccgggttgtc
     1801 ctcgtcggtc acccgcagac caggcatcag atccccggtg gtcatgaaca ccacatccgt
     1861 ttcgccgagc gacggcagca cgcgcccgat gtggtgaagg aacgccgggt tcggcccgac
     1921 cacgagcacg ccgtggttct cgatgcgctt gcgctgggtg tagagcaggt aggcgacgcg
     1981 gtgcagggcc accacggtct tcccggtgcc cgggccgccc tcgatcacca agacgccggg
     2041 atggtcgagc cggatgatct cgtcctgctc ggcctgaatg gttgcgacga tgtcgcgcat
     2101 cccctcgccg cggggcgcgt tgaccgccgc gagcagggca gcgtcgctgc cgcctcctga
     2161 cccggtcccg ctgggcctgc ccagctcttc gtcggtgaag tcgatgaccc gccgcccgcg
     2221 ggtgtggaac tggcggcggc gacgcatgct ctccgggttc gcgccggtgg cggtgtagaa
     2281 cggccgcgcc gcgggcgcgc gccagtcgat cagcaacggc tcgtattcgt
//
LOCUS       DASYLX010001140         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1847057,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001140 DASYLX010000000
VERSION     DASYLX010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847057"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VGP27_27115"
     CDS             <1..604
                     /locus_tag="VGP27_27115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEV7855004.1"
                     /translation="SAIAARHSVRLAVVTAMQELPARQRAVLILRDVVQFSAAEVAEL
                     LETTPAAVNSALARARTHLAEAAPTEDDVADPDDAALRSLLDRYCAAFENGDVTALTE
                     LLRADVKLEMPPHPVWFDGRDAVMRFLAARAFSGRGDVAMVPTAANAQPAVADYRRRA
                     DGILAAHALHVLTVGDGGIAAITVFLEPELFASFGLPPTR"
     gene            complement(728..1003)
                     /locus_tag="VGP27_27120"
     CDS             complement(728..1003)
                     /locus_tag="VGP27_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855005.1"
                     /translation="MPIGDAAADAAADGLQAQYMRRCLSNERADLAGRLGKHARLLNE
                     SISSGQTRNAGHLRGHVRRLENEILEINRMLDALARRFPDRQAGSKA"
     gene            complement(1054..1788)
                     /locus_tag="VGP27_27125"
     CDS             complement(1054..1788)
                     /locus_tag="VGP27_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV7855006.1"
                     /translation="MVSAQREFTAYGPSHLVVLVIFVIGAVLLVWIGRRQTEAQNRVL
                     GRVMAAVIVAAFAVALVYKLIRPDIQTSIPLQLCDIAELAAAYALWSQRHWAFVLAYY
                     WGLVLSSQALITPDIGTPEEGAPDFPSHLFLTFFTLHVLVVWAAIYLTWGQRRRPRWR
                     DYRFAIVVTLAWAALTFVFNSIAGTNYGYLNRKPPTASVLDVLGPWPLYLLAEVAIVL
                     VVWALMTWPWERTRRPVDEKGEHLYG"
     gene            complement(1909..3102)
                     /locus_tag="VGP27_27130"
     CDS             complement(1909..3102)
                     /locus_tag="VGP27_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV7855007.1"
                     /translation="MNTKSPGWRDDGDDLFENAPCGYLVAGADRRIRRANATLASWVG
                     QSADDLAGRRFTDLLSVGSRIHFETHFAPLLQMRQALSGVAVDLITADGDRLPVFIAI
                     NVRSGTDGKSAEFRMTIDDARDRRSYERELLDMQQRAEEQRQRVEVLARTLQRSLLPP
                     SLSPPAGLEACALFRAASVDDVGGDFYDLFPLSADRWGFFLGDVCGKGAGAAAVTSLT
                     RYTLRAAAVFDDDPVAVLHNLNAVLAQDFLKQSSIGDRSLFSTVIFGIMTRADDGFDV
                     ELAAGGHPCPLLLHSHGSAQEVELHGGQAVGMVADAHFVATRLYLGPGDTLVLYTDGL
                     TEARVGEGAERFDDSGALMQFAQAHAPATPQGICQAVDSLLDDFGAGLEDDVALLALG
                     VPPRP"
BASE COUNT          573 a         1053 c         1022 g          524 t
ORIGIN      
        1 gtcggcgatc gcggcgcgac acagcgtccg gctggcagtg gtcaccgcga tgcaggagct
       61 tcccgcccgt cagcgcgccg tactgatcct gcgcgacgtt gtgcagttca gcgcggcaga
      121 ggtcgccgag ttgctggaga cgactccggc ggctgtcaac agcgcacttg cgcgggctcg
      181 cacgcacctc gccgaggccg cgcccaccga agacgacgtg gcagacccgg acgacgcggc
      241 actccggtca ctgctcgatc gttactgcgc ggccttcgag aacggagacg tcacggccct
      301 caccgaactg ctgcgcgccg acgtcaaact cgaaatgccg ccccaccctg tgtggttcga
      361 cggacgcgac gcggtgatgc gtttccttgc cgcgcgtgca ttctccgggc ggggcgacgt
      421 ggcgatggtc ccgaccgccg ccaacgccca gccggcggtc gcggactacc gacgccgcgc
      481 cgacggcatt ttggcggcgc acgcactaca tgtgctgacc gtcggtgatg gcgggatcgc
      541 tgcgatcacc gtcttcctcg agcccgaatt gttcgcgtcg ttcggtctac cgccgacgag
      601 gtagcgcgag tgtgcggatc tgtacgcatc tgcccggcgt ttcgcggacg aaaccgcaca
      661 ctggcgaccc acccctgatc gccgggtgcg ctgaagccca actccgaagc tcggcgacca
      721 gcgacggtca ggccttgctt ccggcctggc ggtcagggaa ccggcgagca agcgcatcaa
      781 gcatccgatt gatctcaagg atctcgtttt cgagccgccg gacatgaccg cggaggtggc
      841 cggcgttgcg cgtctgtccc gacgatatcg actcattgag caggcgagcg tgcttgccga
      901 gcctgccggc caagtcggcc cgttcgttgc tcagacaccg gcgcatgtat tgcgcctgca
      961 gtccgtctgc ggccgcgtcc gccgccgcgt ctccgatagg catcgaggct gaagctagcc
     1021 gatgaggccg cggcgtgcag cggctgggtg tcttcacccg tacaggtgct cacccttttc
     1081 gtcgaccggt cgccgtgtcc gctcccacgg ccaggtcatc agcgcccata ccacgaggac
     1141 gatcgcgacc tctgccagca gatagagcgg ccacggtccc agcacgtcca gcaccgaggc
     1201 agtgggtggc tttctgttga gatagccgta gttggttccc gctatcgaat tgaagacgaa
     1261 cgtcaacgcc gcccatgcaa gggtcacgac gatcgcgaag cggtagtcgc gccaacgcgg
     1321 ccgcctgcgt tgcccccacg tcagatagat cgcggcccac acaacgagaa catgcagcgt
     1381 gaagaacgtg agaaagagat ggctcgggaa gtccggcgcc ccttcctccg gcgtgccgat
     1441 gtccggtgtg atcagcgcct gtgagctcag caccagaccc cagtagtagg cgagcacgaa
     1501 ggcccaatgt cgctgggacc acaatgcgta agcggccgcg agttccgcga tgtcgcagag
     1561 ctgcagcggt atcgaggtct ggatgtctgg ccggatcagc ttgtacacca gcgcgacggc
     1621 gaacgccgcg acgatcacgg ccgccatcac gcggccgaga acccggttct gcgcttcggt
     1681 ctgccttcgc ccgatccaca ccaggagaac agcaccgatc acgaagatca cgagcaccac
     1741 caagtgcgac ggcccgtacg ccgtgaactc ccgttgtgcc gagaccactc gtcaactccc
     1801 gtttcgatga gagatttatg accaatcatt tcagagggat cggccggcgc gcggattacg
     1861 aacggaaatg tcgattcgcc gagcgagagc gctcgtcatc ccgcgatgtc aggggcgcgg
     1921 cggcacccct aatgccagta gtgcgacgtc gtcctccaga ccggctccga agtcgtcgag
     1981 cagcgagtcg acggcttgac agatgccttg gggcgtcgcc ggtgcatgag cctgggcaaa
     2041 ttgcatcagg gcgccggagt cgtcgaaccg ttcggcaccc tcgccgacgc gggcctcggt
     2101 gagcccgtcg gtgtagagca ccaacgtgtc accgggcccc aggtacagtc gcgtggcgac
     2161 gaaatgtgcg tcggccacca tccccaccgc ctgaccacca tgaagttcca cctcctgggc
     2221 ggaaccatgg ctatgcagaa gcaacgggca cgggtgaccg cccgccgcca actcgacgtc
     2281 gaacccgtca tcggcgcgag tcatgatgcc gaaaatcacc gtcgagaaca gggaccggtc
     2341 accgatagaa gattgtttca ggaaatcctg tgcgagaacg gcattgagat tgtgtagcac
     2401 tgctaccggg tcgtcgtcga agactgcggc ggcgcgcagg gtgtagcggg tcaacgacgt
     2461 gacggcggcg gcgccggcgc ccttgccgca tacatcaccg aggaagaaac cccaacggtc
     2521 cgcgctcagg ggaaacaggt catagaagtc accgccgacg tcgtcgaccg acgccgcgcg
     2581 gaacagcgca cacgcctcga gaccggccgg tggtgagagc gaaggcggca gcagcgaccg
     2641 ttgcagtgtg cgcgccagga cctcgaccct ctgcctttgt tcttcggcac gctgttgcat
     2701 gtcgaggagt tcgcgttcgt aggagcgtcg atctcgtgcg tcgtcgatcg tcatacggaa
     2761 ctccgccgat ttcccgtcag tgccggatcg cacgttgatg gcgatgaaaa ccggcaggcg
     2821 gtcgccgtca gcggtgatca gatcgaccgc cacgccgctc aatgcctggc gcatctgcag
     2881 caggggcgcg aagtgcgtct cgaagtggat ccggctgccg acactgagca gatcggtgaa
     2941 tcgacgacca gccagatcgt ctgcactctg ccctacccac gatgccaacg tggcgttcgc
     3001 ccggcggata cggcggtcgg ctccggcgac cagataaccg cacggcgcgt tttcgaagag
     3061 gtcgtctccg tcgtcgcgcc accccggtga cttcgtgttc accgttgcag gaatgccact
     3121 attgcctccg ccgtggcatc cggggcgctc agctgcgggc agtggccgtc gg
//
LOCUS       DASYLX010001141         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2505627,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001141 DASYLX010000000
VERSION     DASYLX010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505627"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGP27_27135"
     CDS             complement(<1..215)
                     /locus_tag="VGP27_27135"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEV7855008.1"
                     /translation="MKKLSLAGGIGGLAAACVAALIAASLSSGSSHREAPNIMLDPSA
                     DNTDTYAFTANDAPDKLTVVANWVPGG"
     gene            386..1408
                     /locus_tag="VGP27_27140"
     CDS             386..1408
                     /locus_tag="VGP27_27140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855009.1"
                     /translation="MTVRVRILSALTAIALAAIVLVVVRTSGGSPDEPSHARLAATNV
                     GSPIKVGIDPVAVAVGAGAIWVIDAARQTLIKVNPHTRAVVSDPRRVAGGPFAVAVGA
                     GVVWVAAGDGSVRAYDPRSLRPTGPTATVPGANGLAVGGGGVWVSSRRAGTVTRIDPR
                     TRHTDQPIRVGRGPADIAAGLGAVWVANADGGSVSRIDPHRGRADTPIAIGARQVLAV
                     TVGDGAVWVARAAGPDADRTQLVHIDPQAGKVVGDPIRIPGAVPLDLVAGDGVWATDS
                     GGVRTLQSGSRGGVTRVDPATRKVAGPLLRTGTRPAAIALGDGAVWVTNEDAGTLTPI
                     TAARRR"
     gene            1518..>3228
                     /locus_tag="VGP27_27145"
     CDS             1518..>3228
                     /locus_tag="VGP27_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855010.1"
                     /translation="MRRPMVRWFDPHQLVDTAVRVLVSGMFSSYADNREVQAREPAEV
                     LDRSEHPDLWLDYVADLGDGWNSTYTVARLLATEELELDWDGETHATERGRILVMGGD
                     QVYPVPKAAEYENRMLGPYRAALPCARGEAPELFAIPGTHDWYDGLFNFTSIFCRNRW
                     IGGWRTCQRRSYFALKLPNGWWLWGIDIQFGASIDEAQLQYFADIAADQMHPGDRVVL
                     CMSKEVQSGRKQDEIHSDRDVAYLEREIIQPSGAQLLLYLKSGKHYYSRYEEQDGPRH
                     HIESGGGGAFLHPTHNLPERIDLPGPDGPTGYQRADTYPSAAVSRRLRKRIWLIPAYN
                     LPLAAVFGTVQVLLAFMLGLHLHERHESLGVGDLLQAMWQSPTAFLLSLLVLASLVGM
                     VRFAHDASGIGRVLLGLTHSALQVASVAGVMIAASYLSSAGGLDGAWSLLAFLGLVWL
                     LGGISGMVGMAGYLWATGCLGLHGTEAYAPLHHQDLKHFLRLHIQADGTLTLYPIGID
                     RVGRTWTLRPDAPAHAPWFAPDGPEPEPHLIEQPITIGQTDAHAPGTTTSSSRAATAS
                     RRSR"
BASE COUNT          512 a         1167 c         1048 g          501 t
ORIGIN      
        1 ttgccgcccg gcacccagtt ggcgacgacg gtcagcttgt ccggagcatc gttggcggtg
       61 aacgcgtacg tatcggtgtt gtccgcagac gggtcgagca tgatgttcgg cgcctcgcga
      121 tgactcgaac ccgaactcaa cgacgcagcg atcagcgccg cgacacacgc ggctgcgaga
      181 cctccaatac ctccggccag ggacagcttc ttcacgggat ctcctccgcc gacgtggatg
      241 cgcggccgtg cgcccggttc cggcatgacg gccggttcaa gtcagtctgc gcgccagcat
      301 gtcgcgcgga tgcctaccgg tgaaagctgg cgcgcgcaca cccacagtga tctgtttgac
      361 ccgtacacgg cgaatggcgg tggagatgac cgttcgcgtc aggatcttga gtgcgctcac
      421 ggcgatcgcc ctcgcggcga tcgtcctcgt cgtcgtccgc acaagcgggg gctctcccga
      481 cgagccgtcg cacgcacgac tcgcggccac gaacgtcggc tcaccgatca aggtcggcat
      541 cgacccggtc gcggtggcgg tcggagccgg cgcgatctgg gtcatcgacg ccgcacgcca
      601 gacactcatc aaggtcaacc cgcacacgcg ggccgtcgtc agcgacccgc gacgcgtcgc
      661 cggcggcccg ttcgccgttg ccgtcggtgc gggcgtcgtg tgggtcgcgg cgggagacgg
      721 atccgtgcga gcctacgacc cgcgcagcct ccggccgacc gggccgaccg ccaccgtgcc
      781 cggcgccaac gggctggcgg tcggcggcgg cggcgtctgg gtctccagcc ggcgcgccgg
      841 aaccgtcaca cgcatcgacc cgcgcacacg ccacaccgac caaccgatcc gcgtcggccg
      901 cggccccgcc gacatcgccg ccggcctcgg cgccgtctgg gtcgcaaacg ccgacggtgg
      961 cagcgtcagc cgcatcgacc cacaccgcgg acgcgccgac acacccatcg ccatcggcgc
     1021 ccgccaagtc ctggccgtaa ccgtgggaga cggcgctgtg tgggtcgccc gcgccgcagg
     1081 ccccgacgcc gaccgcacgc aactcgtgca catcgacccc caggcgggca aggtcgtggg
     1141 cgaccccatc aggatcccag gcgccgttcc actggacctc gtcgccgggg acggcgtctg
     1201 ggcaaccgac agcggcggtg tgcgcactct ccagtctggc agccgtggcg gcgtgacccg
     1261 cgtagacccc gcaacacgaa aggtggctgg accacttcta cgcactggga cacggccggc
     1321 ggcgatcgcc ctcggcgacg gcgccgtgtg ggtcaccaac gaggacgctg gcacgctcac
     1381 cccaatcacg gccgccagac gacgctgagc gcgcgatcaa ggctccacag tccagcatga
     1441 ccgaaacacc tgctgatcgc gcggacacgc cctcgccgtg tgttcctcgc ccgtccgtcc
     1501 cttctgagct tggcttcgtg cgcaggccga tggtgcgctg gtttgatcct catcagctcg
     1561 tcgacacggc cgttcgcgtt ctggtctcgg ggatgttcag ctcctacgcc gacaaccggg
     1621 aggtgcaggc gcgggagccg gccgaggtgc tggatcgctc cgagcaccca gatctgtggc
     1681 tcgactatgt ggccgatctc ggcgacgggt ggaactcgac gtacacggtg gccaggctgc
     1741 tggcgaccga agagctcgag cttgactggg acggcgagac ccacgcgacc gagcgcgggc
     1801 ggatcctcgt catgggcggc gatcaggtgt acccggtgcc caaggcggcg gagtacgaga
     1861 accgtatgct gggcccgtac cgggcagcgc tgccgtgtgc ccgcggagaa gctcctgagc
     1921 tgttcgcgat tccgggaacg cacgactggt acgacgggct gttcaacttc acgagcatct
     1981 tctgtcgcaa ccgctggatc ggcggatgga ggacgtgcca gcgccgcagt tacttcgcgc
     2041 tcaagcttcc caacgggtgg tggctgtggg gcatagacat ccagttcggg gcttcgatcg
     2101 acgaagcgca actgcagtac ttcgccgata tcgccgctga tcaaatgcac cccggggacc
     2161 gggtcgtcct atgcatgtcc aaggaggttc agagcggccg caaacaggac gagatccatt
     2221 ccgaccgcga tgtggcgtac ctcgagcgcg agatcatcca gcccagcggt gcccagttgt
     2281 tgctgtacct caagagcggc aagcactact actcgcgcta cgaggagcag gacggcccgc
     2341 ggcaccacat cgagtcgggc ggcggcggcg cgttcctgca cccgacccac aacctccccg
     2401 agcgcatcga cctccccgga ccagacggac ccaccggcta ccagcgagcg gacacctatc
     2461 cgtcggcggc ggtatccaga cggctgagga agcggatctg gctgatccct gcctacaacc
     2521 ttccgcttgc cgcggtgttc ggcaccgtcc aagtcctgct ggcgttcatg ctgggactac
     2581 atctccacga gcgccatgag tcgctcggcg tcggtgacct gcttcaggct atgtggcaga
     2641 gcccgaccgc cttcctgctc agcctgctcg tgttggcttc cctggtcggg atggtgcgct
     2701 tcgcccacga cgccagcggg atcgggcgcg tcctgctcgg gctgactcac tcggcgctac
     2761 aggtagcaag cgtcgccggc gtcatgattg ccgcctcgta tctgtcctcc gccggcgggc
     2821 ttgacggcgc gtggtccctg ctcgccttcc tcggcctggt ctggctgctg ggcggcatct
     2881 cgggcatggt cgggatggcg ggctatctgt gggccaccgg ctgccttggc ctccacggca
     2941 ccgaggccta cgcgcccctg caccaccaag acctcaagca tttcctgcgc ctgcacatcc
     3001 aggccgacgg aaccctgacc ctgtacccga tcggcatcga ccgcgtcggc cgcacatgga
     3061 cactgcggcc cgacgcgcct gcgcacgccc cttggttcgc ccctgacggg cctgagcccg
     3121 agccgcatct catcgaacag ccaatcacaa tcggacaaac cgacgctcac gccccgggca
     3181 cgaccacatc atcctcacgg gccgctaccg catcccgccg tagccggc
//
LOCUS       DASYLX010001142         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3272713,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001142 DASYLX010000000
VERSION     DASYLX010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272713"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1647
                     /locus_tag="VGP27_27150"
     CDS             <1..>1647
                     /locus_tag="VGP27_27150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7855011.1"
                     /translation="LGRPFRDRRRLTIPGVATAADGLVALGCGTAQQWFDLCAMVGHD
                     EWIDEQSEFSITEQANIHAEQIYDWVRDNNVDDILELSSAFRIPNAPVGNGANVTSFD
                     QFAERDTFVANPRDAFTQPGPPYRATPPLVRAPQPAPRLGEHTDHYRRNRHMGVKKGE
                     ISAQTDIWAGFSLEGVRVLDMTSFWAGPSCTHVLAQLGAEVIHVESTARPDGTRLIAG
                     VPITEDQWWERSPIFSGLNTNKKSVTLDIRNERGVELLHQLVTTCDVIVENYTPRVLD
                     QIGLDFDTVHRLRPDAIMMRMPGFGLDGPWRDKPAFAYVIEDASGITWLTGHPDQNPV
                     EPYAVGDPNAGVHGLNALLLAMAHRRRTGQGVRIEAAMVDAALNVAAEQVIEYSAYGS
                     LLQRQGNRGPTAAPQNLYRTGELDEFGRPDDWVAIAVATDEQWVGLVTALGNPEWVTD
                     DMACAVGRRRHHDEIDEHLSRWCGTRTGEEIVESLWTAGVPVAKVMQPHRVGDLPQVV
                     HRGFYERVDHPVNPTARHSTLPMRISTGPQRFHRTPAPLLG"
BASE COUNT          296 a          584 c          528 g          239 t
ORIGIN      
        1 ctgggccgac cgttccgcga tcggcgtcgg ctcacgatcc cgggtgtcgc gacggccgcc
       61 gacggtctgg tcgcgctggg ctgcggcacc gcacagcagt ggttcgacct gtgcgccatg
      121 gtcggccacg acgagtggat cgacgagcag tcggaattct ccatcaccga gcaggccaac
      181 atccacgccg agcagatcta cgactgggtt cgcgacaaca atgtcgacga catcctcgag
      241 ttgtcctccg cgttccggat ccccaacgca ccggtcggaa acggcgccaa cgtcacctcg
      301 ttcgaccagt tcgccgaacg ggacacgttc gtcgccaacc cgcgcgacgc gttcacccag
      361 cccggaccgc cctatcgcgc gaccccgccg ctcgtgcgtg cgccgcaacc cgccccacga
      421 ctcggcgagc acaccgacca ctaccgccga aaccgacaca tgggcgtgaa aaagggcgaa
      481 atcagcgccc aaaccgacat ttgggcgggg ttttcgctcg agggtgtgcg cgtactcgac
      541 atgacgagct tctgggccgg gccgtcgtgc actcatgtgc tggcacagct cggcgccgag
      601 gtcatccacg tcgagtcgac ggcgcgtccc gacggcacgc ggttgatcgc gggcgtgccg
      661 atcaccgagg accagtggtg ggagcgctcg ccgatcttct ccggcctgaa caccaacaag
      721 aagagcgtca cgctggacat ccgcaacgaa cgcggcgtcg agctgctcca ccagctcgtc
      781 acgacgtgcg acgtcatcgt cgagaactac acgccgcggg tgctcgacca gatcgggctc
      841 gacttcgaca ccgtgcaccg gctgcgcccc gacgccatca tgatgcgcat gcccggcttc
      901 gggctcgatg gcccgtggcg ggacaaaccg gccttcgcct atgtcatcga ggacgcatcc
      961 ggaatcacct ggctcaccgg ccatcccgac cagaatcccg tcgaaccgta tgccgtcggc
     1021 gaccccaacg caggtgtgca cgggctcaac gcgttgctgc ttgcgatggc gcatcggcgg
     1081 cgcacggggc agggcgtccg gatcgaggcc gcgatggtcg acgccgcgct caacgtcgcc
     1141 gccgaacagg tcatcgagta ctccgcctac ggcagtctgc tgcagcgaca gggcaaccgc
     1201 ggtccgaccg ccgcaccgca gaacctctac cgcaccggcg aactcgacga gttcgggcgg
     1261 cccgacgact gggtggcgat cgcggtcgcc accgacgagc agtgggtcgg tctggtcact
     1321 gcgctcggca acccggaatg ggtgaccgac gacatggcct gcgccgtcgg acgaagacgg
     1381 caccacgatg agatcgacga gcacctgagc cggtggtgtg gaacccggac aggggaggaa
     1441 atcgtcgaat ccctctggac ggcaggcgta cccgtggcga aggtcatgca gccgcaccgg
     1501 gtcggcgact tacctcaagt ggtgcatcgc gggttctacg aacgggtcga ccaccccgtg
     1561 aatcccacgg cgcggcacag cacgctgccg atgcgcatct cgactgggcc gcagcgcttt
     1621 caccggacac ccgcgccgct tctcggg
//
LOCUS       DASYLX010001143         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_970878,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001143 DASYLX010000000
VERSION     DASYLX010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970878"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VGP27_27155"
     CDS             <1..784
                     /locus_tag="VGP27_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEV7855012.1"
                     /translation="VFVDAGFSREQVIAVARVLGQGMAQTAEVMRQVVLEAVIEPGAT
                     EVQIAKAYGELVQQVSPLLGPLAEDVLRLQLRHGLETEAVSVAERAAGKLPGAREVTV
                     CFADLVGFTRLGEAVPPEELESLANRLSALARDVAAPPVHFIKTIGDAVMFVSTDAAA
                     LLRAGLELLAAAEKEDLPQLRVGLAAGSAVRRAGDWFGSPVNLASRVTAAARPGSVLV
                     AEPVHEQIGDTDEFSWSFAGARHLKGIKGEVKLFRARAAGTE"
     gene            830..>1663
                     /locus_tag="VGP27_27160"
     CDS             830..>1663
                     /locus_tag="VGP27_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEV7855013.1"
                     /translation="MLDGLEGDARQERIDLISWLLTRGFRLDHIRGSVGAPLSLPANR
                     VIGDDGEYVSARQICQSTGIDLGLLRRIHRAIGLPRIADPDAPNLLRVDGEAAAQARI
                     ILDLGISPEDVVAVLRVMRESLGHAAAVMREAGLKAILKPGATEIEVAGAIEALALRA
                     APSFGPMMEALLRLELRRSFETEAVNAAERSAGILPGARRMTVCFADLTGFTRLGEAL
                     PPEDLERVANRLAELAHDLSRAPVRFVKSIGDAVMLVCSDALPLVVAVLDLVAAAAAN
                     DL"
BASE COUNT          246 a          503 c          622 g          292 t
ORIGIN      
        1 ggtcttcgtc gacgcaggct tcagccgcga gcaggtgatc gctgtggccc gcgtgctcgg
       61 acagggcatg gcgcagacgg ccgaggtgat gcggcaggtg gtgctcgagg cggtgatcga
      121 gccgggcgcg acggaagtcc agattgccaa ggcctacggc gaactggtgc aacaggtttc
      181 gccgctgctc ggacctttgg ccgaggacgt gctgcgcttg cagcttcgcc atgggttgga
      241 aaccgaggcg gtcagcgtcg ccgagcgagc ggcgggaaag ctgcctggcg cgcgtgaggt
      301 gactgtctgc ttcgccgacc tcgtcggctt cacccggttg ggcgaggcgg tgccgccgga
      361 ggagttggag agcctcgcga atcggttgtc cgcgctcgcc cgcgacgtcg cggcgccacc
      421 cgtgcacttc atcaagacga tcggcgacgc ggtgatgttc gtcagcacgg acgcggccgc
      481 gttgttgcgt gccgggctcg agctgttggc cgccgcggag aaagaggacc tgccgcagtt
      541 gcgtgtcggc ctggccgcgg gcagtgcggt ccggcgggcg ggggactggt tcggcagccc
      601 ggtcaacctg gcgagtcggg tgaccgcggc ggcccggcca ggatcggtgc tcgtcgccga
      661 accggtgcac gagcagattg gcgataccga cgaattctcc tggtcgttcg ccggcgcgcg
      721 gcacctcaaa gggatcaagg gcgaggtgaa gctgtttcgg gcacgcgcgg cgggtacgga
      781 atagtacgtt caccccatgg cttcggacgc cgagttcgag gcgttgggca tgctcgacgg
      841 cctggagggc gatgcgcggc aggagcgcat cgacctgatc agttggctgc tcacccgagg
      901 tttccggctc gaccacattc gcggttcggt gggagcgccg ctgagcctgc ccgccaaccg
      961 ggtgatcggc gacgacggtg agtatgtgtc cgcgcgccaa atatgccagt cgaccggaat
     1021 cgatctgggt ttactgcgac ggatccatcg tgcaatcggc ctgcctcgca tcgcagatcc
     1081 tgacgcaccg aatctgctgc gcgtcgacgg cgaggccgcc gcgcaagcac ggatcattct
     1141 cgacctgggt atcagtcctg aggacgtggt cgcggtcctg cgcgtgatga gggagagcct
     1201 cggccatgcg gctgcggtga tgcgtgaagc ggggctgaag gcgattttga aaccgggcgc
     1261 cacggagatc gaagtggccg gggcgattga ggcactggcg ctgcgggcgg cgccgtcttt
     1321 cgggccgatg atggaggccc tgctgcggct ggagttacgc cgttcgttcg agaccgaagc
     1381 ggtaaatgcg gccgagcgct ccgccggaat actccccggc gcccggcgga tgacggtgtg
     1441 ctttgccgac ctaacgggct tcactcgact tggtgaggcg cttccgccgg aagaccttga
     1501 gcgcgttgcc aaccggctcg cggaactggc ccacgactta tcacgtgcac cggtacggtt
     1561 cgtcaagtcc atcggcgatg cggtgatgct ggtgtgctcg gatgccctcc cgctggtcgt
     1621 tgcggtgctg gacctggtgg ccgccgccgc cgcgaacgac ttg
//
LOCUS       DASYLX010001144         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2397922,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001144 DASYLX010000000
VERSION     DASYLX010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397922"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..866
                     /locus_tag="VGP27_27165"
     CDS             81..866
                     /locus_tag="VGP27_27165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7855014.1"
                     /translation="MSDGEQAKPRRRRGRRRGRRAAGPPDTANASHNNNSVVPENNTA
                     PVPDGRPKPHKPRGARRGPDRLRTVHETSAGGLVIDGLDGPKDTQVAALIGRVDRRGR
                     MLWSLPKGHIELGETAEQTAIREVAEETGIQGTVLAALGSIDYWFVTEGRRVHKTVHH
                     YLLRFLGGELSDEDVEVSEVAWVPLKELPARLAYADERKLAEVADELIDKLHTDGPSA
                     LPPLPRSSPRRRAQTHSHTRNRRPDEAGPPKPGRRTNGCGPGP"
     gene            863..>1612
                     /locus_tag="VGP27_27170"
     CDS             863..>1612
                     /locus_tag="VGP27_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855015.1"
                     /translation="MTACRGSGTVLPRLLAVLALLILIATPAVSPHAAAGEPGSVPFL
                     QVRIDRVTPEVMTTTSDPVVTVSGTVLNVGDRPVRDVMVRLEHAAAVSSSAGLRTNLG
                     GDNDQFEAVADFITVSAELQRGQNVPFNLTVPLRSAQTPSLRIDTPGVYPLLVNANGT
                     PDYGAPARLDDARFLLPVIGVPPEPAANSSPDALADVVAPDTSKPVRTTVMWPLGDRP
                     RLAPGAPGGSTPVRLTDDELATELAVGGRLDV"
BASE COUNT          262 a          604 c          537 g          209 t
ORIGIN      
        1 aatgagcgta gaggtaacgg tcctgcaccg agcggcgagt gtggcgggag cgaattctgg
       61 tggcagctac tatcgcttgg gtgtcggacg gcgaacaggc caaaccacga cggcgccgcg
      121 ggcgtcgtcg cggtcgtcgc gcggccggtc cacccgacac cgccaatgca tcgcacaaca
      181 acaattcggt ggtgccggag aacaacaccg ccccggtccc cgacggcagg ccgaagccgc
      241 ataaaccgcg cggtgcccgc cgcggccccg accggctccg cacagtgcac gagacatcgg
      301 cgggcggcct ggtcatcgac ggtctcgacg gcccgaagga cacccaggtc gcagccctga
      361 tcggacgggt ggaccgccgc ggccgaatgc tgtggtcgct gcccaagggc cacatcgaac
      421 tcggtgagac cgcagaacag accgcgatcc gcgaggtcgc cgaagagacc ggcatccagg
      481 gcaccgtgct ggcggccctc ggcagcatcg actactggtt cgtcaccgag ggcaggcggg
      541 tgcacaagac cgtgcaccac tacttgttgc gattcctcgg cggcgagctg tccgacgaag
      601 atgtcgaagt cagcgaggtg gcgtgggtgc cgctcaagga gctgcccgcc cggctcgcct
      661 atgccgacga acgcaagctc gccgaggtcg ccgacgagtt gatcgacaag ctgcacaccg
      721 acgggccctc cgcattgccg ccgctgcccc gcagttcgcc ccgcaggcgc gcccagacgc
      781 actcgcacac ccgcaaccga cgtcccgacg aggcaggccc acccaaaccg ggccggcgga
      841 cgaacggctg cggaccaggg ccgtgaccgc ctgccggggg tccggcacgg tgctgccccg
      901 tctgctcgcc gtgctggcac tgctgatcct gatagccacc cccgcggtgt cgccgcacgc
      961 ggccgcgggc gaaccgggct ctgtgccgtt cctgcaggtg cgcatcgacc gcgtcacgcc
     1021 cgaggtgatg acgaccacca gcgatcccgt cgtcaccgtc tccggaacgg tgctcaacgt
     1081 cggcgaccgc ccagtccggg acgtgatggt ccgactcgag cacgccgcgg ccgtcagctc
     1141 ctctgccggg ctccgcacga acctcggcgg cgacaacgac caattcgagg cggtcgccga
     1201 cttcatcacc gtttccgcgg aattgcaacg cggacagaac gttccgttca acctgacggt
     1261 tccgttgcgc tcggcgcaga cgccgtcgct gcgcatcgac acgcccgggg tgtacccgct
     1321 gctggtcaac gccaacggca cccccgacta cggcgccccc gcccgcctgg acgacgcccg
     1381 cttcctgcta ccggtgatcg gggttccgcc cgagcccgcc gcgaactcgt cgcccgacgc
     1441 gctggccgac gtcgttgcgc ccgacacgtc gaaacccgtc cgcacgaccg tgatgtggcc
     1501 gctcggcgac cggccacggc tggcgcccgg ggcgcccggc ggctcgacac cggtgcggct
     1561 gaccgacgac gaactcgcca ccgaactggc ggtcggcggg cggctcgacg tg
//
LOCUS       DASYLX010001145         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2506300,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001145 DASYLX010000000
VERSION     DASYLX010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506300"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGP27_27175"
     CDS             complement(<1..390)
                     /locus_tag="VGP27_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7855016.1"
                     /translation="MDAVDNRTEIRDFLATRRAKLTPEQVGLPTSGRRRVPGLRREEV
                     AVLAGVSPDWYIRLEKGHISGVSDAVLDAVARALQLHDAERDHLFNLARAARPVRTPR
                     RRGRTAIPPSVQRVLDSMTGTAAFVRNG"
     gene            483..1946
                     /locus_tag="VGP27_27180"
     CDS             483..1946
                     /locus_tag="VGP27_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7855017.1"
                     /translation="MTATPTTSSPTGATAATPSSVPATAGPASTSPAAGSARAGGDRH
                     ASFALLAALLGFFVVTLDAVVVNVTLPTIRGELGGGVTGLQWVVDSYTLMFAALLLTA
                     GSLSDRLGARRAFGAGMVVFVLASVGCGLAPSLGVLIVARFVQGAAAAAMMPASMALI
                     RQAFPDAKARGRAVGVWAMGGAVASSSGPVLGGVLNLVDWRLIFFINVPVGAIALLLL
                     ARVRPSQPRVVPFDLIGQVTPVLAMAGLTFGAIEAGANGFRDPAVIAAFTVAAAALAG
                     FVRAEQVVAHPMMPLQLFRSRTVVITVVSGFAFMVGYYGLPFVISLFLQQHRGLDALQ
                     TGVVFLPMMLIGLVLTPFSARMSERLGRKTLIVAGLLLMAAGLAVIGLLPASTPLWLL
                     AGLMVLVGLGGPTVSPPATTVLLDSVPEAQSGVASGVFNTSRQVGGALAIAVFGGLLA
                     DPVTFVHGVHTSLLIAAAVIALTTVLALFLPTTKEHS"
BASE COUNT          250 a          748 c          750 g          370 t
ORIGIN      
        1 gccgttgcgg acgaacgcgg cggtgccggt catcgagtcg agaacgcgct gcacgctggg
       61 cgggatcgcc gtcctcccgc gccgtcgggg ggtacggacg ggtctggcgg cgcgggcgag
      121 gttgaacagg tggtcgcgtt ccgcgtcgtg gagttgcagt gcccgggcca cggcgtcgag
      181 gacggcgtcg gacacgccgc tgatgtggcc cttctccagg cggatatacc agtcggggct
      241 gaccccggcg agcacggcga cctcttcgcg gcgcagcccg ggcactcggc gccgcccgga
      301 ggtcggtagc ccgacctgct cgggggtgag cttggcgcgc cgggtggcga ggaagtcgcg
      361 gatctcggtg cggttgtcca cggcgtccac gctacgagca tctgcggcgt aagggaggtc
      421 gagcttgtac ccccgataac tcgccctgcc acgcgcgcga gaaccgcggt tgcctagatg
      481 acatgaccgc gacacccacc acgtcgagcc ccacgggggc gacggctgcg acaccctcga
      541 gcgttcccgc gacggcaggt ccggcatcga ccagcccggc tgccggctcg gcgcgcgcgg
      601 gcggggacag gcacgccagc ttcgccctgc tcgcggcgct gctcgggttc ttcgtggtca
      661 ccctggatgc ggtggtggtc aacgtgacgc tgccgacgat ccgcggggag ctgggcggcg
      721 gagtcacggg cctgcagtgg gtggtcgaca gttacacgct gatgttcgcc gcgttgctgc
      781 tcacagccgg gtcgctgtcc gaccggctcg gcgcgcggcg cgcgttcggc gcgggaatgg
      841 tcgttttcgt cctcgcctcg gtggggtgcg gtctcgcccc gagcctgggc gtgctgatcg
      901 tggcccggtt cgtgcagggc gcggccgcgg cggcgatgat gcccgcctcg atggcgctga
      961 tccgtcaagc gttccccgac gcgaaggccc gcgggcgtgc tgtcggggtg tgggccatgg
     1021 gcggtgcggt ggcgtcctcc tcgggtccgg tgcttggcgg cgtgctgaac ctcgtcgact
     1081 ggcggctgat cttcttcatc aacgtcccgg tcggcgcgat cgccttgctg ttgttggccc
     1141 gcgtccgccc ctcgcagccg cgtgtcgtgc cgttcgacct gatcgggcag gtcacccccg
     1201 tgctggcgat ggccgggctg acgttcggcg ccatcgaggc cggagcgaac gggttccgcg
     1261 accctgccgt gatcgccgcg ttcactgtcg ccgcggctgc gctggccggg ttcgtccgcg
     1321 cggagcaggt tgtcgctcac ccgatgatgc cgctgcagct gtttcgctcc cgcaccgtgg
     1381 tcatcacggt ggtcagcggg ttcgcgttca tggtcggcta ctacggcctg ccgttcgtga
     1441 tcagcctgtt cctgcagcag catcgcggtt tggacgcgct gcagaccgga gttgtgttcc
     1501 tgccgatgat gttgatcggc ttggtgctca ccccgttctc ggcgcgcatg agcgaacgtc
     1561 tcggccgcaa gaccctcatc gtcgccggcc tgctgctcat ggccgccggg ctggcagtga
     1621 tcggcctgct gcccgcgtcg acaccgctgt ggctgctggc cgggctgatg gtgctcgtcg
     1681 ggctcggtgg accgaccgtc tcaccgccgg cgaccaccgt tctgctggac tccgtgcccg
     1741 aagcccagtc cggtgtcgcc tccggagtgt tcaacaccag ccgccaggtc ggcggcgcac
     1801 tcgccatcgc cgtgttcggt ggcctcctgg ccgaccccgt cacgttcgtg cacggcgtgc
     1861 acaccagcct gctcatcgcc gccgccgtga ttgccctgac caccgtgctc gccctgttcc
     1921 tgcccaccac caaggagcac tcatgaccag ccacacaacc agcaccagca ccggcggcgg
     1981 ctcgaatccc tccgcctggc cagcccacac actggaccgg ttcggcgccg ccgacgagat
     2041 cgacatcagc acccgccgcg acgacgacaa cctgcgcggg ttcgtgccga tctggatcgt
     2101 caccgtcgac aacgccct
//
LOCUS       DASYLX010001146         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_92303, whole
            genome shotgun sequence.
ACCESSION   DASYLX010001146 DASYLX010000000
VERSION     DASYLX010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92303"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGP27_27185"
     CDS             <1..873
                     /locus_tag="VGP27_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7855018.1"
                     /translation="AAKTAGGNKIAVFSDDMSLKGEFRNDIELHLQSAIENGALVLHY
                     LPEVDMRTGEILAFEALVRWQHPTRGLLSPNAFIGVAESINFAGELGRWVMRAACAEF
                     RRWRSKGIGEDVVLRINVSPVQLVTEGFVETVASIIDEFGLDGGSVCLEITESVVVQD
                     IETTRITLAGLKEVGVQIAIDDFGTGYSVLSHLKSLPVDTLKIDKSFVRELGTNPGDL
                     AIVRAVIALAESFGLELVAEGVETEEAALTLLRHGCHRAQGFLLSRPVAGDAMESLLA
                     QRRIPVQFSVAPVR"
     gene            complement(820..1692)
                     /locus_tag="VGP27_27190"
     CDS             complement(820..1692)
                     /locus_tag="VGP27_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855019.1"
                     /translation="MNWDDEVDVVCTGSGIGGLATAIAAVDAGLDVFVADAAGIQVDD
                     DKTKRYFRELSQNLLPGSHPEPTAATPVRVIDDFAPAPSRSQRVEPFTGSGLQAWAAG
                     CLASPYGFLYSRVSERSEVTMRSSRGEPFEVTPIGSIENGADLRKLILSDWLCVQACN
                     RGIDISMDSPLHRIVFEEGQARGAVVGTPWGPRAVRARRGVIVSTGGHDARAVMPCDV
                     SGYTSLLVSVVRRAPSRFGRVELLTIRPPAPPPHRACTPAHRHLIDAARETRQRQSAN
                     RCYGELHRYPPLGK"
     gene            1947..>2563
                     /locus_tag="VGP27_27195"
     CDS             1947..>2563
                     /locus_tag="VGP27_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7855020.1"
                     /translation="MTEQVDRRADATRLQILLAASQEFARKSYSQVSLDDILAQAQVT
                     KGAMYFHFRSKHELALAIIDRHNAMGYESLNEVLARKLSGLETLVDMSCVIATRDIGD
                     AVGRAGLNLLESIGRADGVQARVVDEWTAAFAEVVRRAVAEGDVLADREPEDVGRLVV
                     SAFLGLRQTTDIDDPQRYFGDLAKIWSFTLPGFANPERIGYFNQF"
BASE COUNT          469 a          799 c          845 g          450 t
ORIGIN      
        1 gcggctaaga ccgcaggcgg taacaagatc gcggtgttct ccgacgacat gtcgctcaag
       61 ggcgagttcc gcaacgacat cgagctgcat ctgcagagtg cgatcgagaa cggcgccctg
      121 gtcctgcact atctgcccga agtggacatg cgtacgggcg agatccttgc gttcgaggcg
      181 ctggtgcgct ggcagcaccc gacgcggggc ctgctctccc ccaacgcgtt catcggcgtg
      241 gcggagtcga tcaatttcgc gggcgagctg ggccgctggg tgatgcgcgc cgcctgcgcc
      301 gagttccgcc gatggcggtc gaaggggatc ggcgaagacg tcgtgctgcg gatcaacgtc
      361 tcaccggtgc agctggtgac cgagggcttc gtcgagacgg tcgcgagcat catcgacgag
      421 ttcggccttg acggcggctc ggtgtgcctg gagatcaccg aaagcgtagt cgtgcaggac
      481 atcgagacca cccggatcac cctcgccggg ttgaaggaag taggggttca gatagcgatc
      541 gacgacttcg gcaccggcta cagcgtgctg tcccacctga agtcgctgcc ggtcgacacg
      601 ctgaagatcg acaagagctt cgtgcgtgag ctcgggacca accccggcga tctggctatc
      661 gtgcgcgccg ttatcgcctt ggcagagtcg ttcggtctgg agctggtcgc cgagggtgtg
      721 gagaccgagg aggccgcgct gacgctgctg cggcacgggt gccaccgcgc tcagggcttc
      781 ctgctgtcgc gcccggtggc cggcgacgcg atggagtcgc tacttgccca acggcggata
      841 ccggtgcagt tctccgtagc accggttcgc tgactggcgc tgccgggtct ctcgtgcggc
      901 atcgatcaga tgccgatgcg ccggagtaca tgccctgtgc ggaggaggag cgggtggccg
      961 gatcgtcaga agctcgacgc ggccgaaccg gctcggcgcc cgccgcacaa cgctgaccag
     1021 caacgacgtg tagccggaga catcgcaggg catgacagct ctggcgtcgt gtccgccggt
     1081 ggagacgatc acgccgcgcc gggcccgcac ggcccgcggt ccccacggtg tgccgacgac
     1141 ggcacccctg gcctggccct cctcgaagac gatgcgatgc agcgggctgt ccatgctgat
     1201 atcgatgccc cggttacagg cctgcacaca caaccagtcg ctcagaatca gtttccgcag
     1261 atccgccccg ttctcgatgg aaccgatcgg agtcacttcg aacggctctc cgcggctgga
     1321 gcgcatggtg acttcgctgc gctcggagac ccggctgtag aggaatccgt atggagaggc
     1381 gaggcagccc gcggcccagg cctgcaatcc cgagcccgtg aacggctcga ccctctgcga
     1441 cctcgacgga gcgggcgcga agtcgtcgat cacgcggacg ggcgtcgccg ccgtgggttc
     1501 cggatgggag ccgggtagca ggttctgcga cagctcccgg aagtagcgct tcgtcttgtc
     1561 gtcgtcgacc tggatgccgg ccgcgtcggc cacgaagaca tcaaggccgg catccactgc
     1621 ggcaatggct gtcgcaaggc ctccgatccc cgaaccggtg caaacgacgt ctacctcgtc
     1681 atcccaattc atagcaatta accttccaat tgccagcagc agaaggattc gacaaaaggg
     1741 cggttccatt gggtaattgc ttatgcaaat gattgctatt gcagattaag gcaacaaact
     1801 ctgtgtctga ttcagcaatg gagtcaccgc gcgcgaacaa tccctccggg ttcgaaccag
     1861 aagtcatcac actggagaga ttacatacct tttgaatcat ctgcggcagg cttggtgact
     1921 tagagtatta aacggtggga ctcacgatga cggagcaggt cgacaggcga gcggacgcga
     1981 cgcgtctgca aatcctgctt gcggcgtcgc aggagttcgc gcggaagtcc tacagtcagg
     2041 tgagcctgga cgacatcctg gcgcaggccc aggtcaccaa gggtgcgatg tacttccact
     2101 tccgctccaa gcacgagctg gcgctggcga tcatcgatcg acacaacgca atgggctacg
     2161 aatcactgaa cgaagtgctc gcccgcaagc tgtccgggct cgagacactc gtcgacatgt
     2221 cgtgcgtgat cgccacccgc gacatcggcg atgccgtcgg cagggccggc ctgaacctgc
     2281 tcgagtcgat cgggcgggcc gatggagtgc aggctcgggt ggtcgacgag tggacggcgg
     2341 cgttcgccga agtggtacgt cgcgccgtcg ccgagggcga cgtcctcgcc gaccgcgaac
     2401 cagaagacgt cggccggctg gtggtctcgg cgttccttgg attgcgccag accaccgaca
     2461 tcgacgatcc gcagcgttac ttcggcgacc tcgcgaagat ctggtcgttc accctgcccg
     2521 gcttcgcgaa cccggaacgg atcggttatt tcaaccagtt cat
//
LOCUS       DASYLX010001147         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_752514,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001147 DASYLX010000000
VERSION     DASYLX010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752514"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="sufB"
                     /locus_tag="VGP27_27200"
     CDS             <1..1238
                     /gene="sufB"
                     /locus_tag="VGP27_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV7855021.1"
                     /translation="RTFDKKPMPNWGSNLEGIHFDNIKYFVRSSEKQAATWDDLPADI
                     KNTYDKLGIPEAEKQRLVSGVAAQYESEVVYHSIREDLEAQGVIFLDTDTALKEHPEI
                     FKQYFGTVIPAGDNKFSALNTAVWSGGSFIYVPTGVHVDIPLQAYFRINTENMGQFER
                     TLIIVDEDAYVHYVEGCTAPIYKSDSLHSAVVEIIVKPGGRCRYTTIQNWSNNVYNLV
                     TKRARAEAGATMEWVDGNIGSKVTMKYPAVWMTGEHAKGEVLSVAFAGEGQHQDTGAK
                     MLHLAPNTSSNIVSKSVARGGGRASYRGLVQVNKGAHGSRSSVKCDALLVDTISRSDT
                     YPYVDIREDDVTMGHEATVSKVSEDQLFYLMSRGMTEDEAMAMVVRGFVEPIAKELPM
                     EYALELNRLIELQMEGAVG"
     gene            1271..2449
                     /gene="sufD"
                     /locus_tag="VGP27_27205"
     CDS             1271..2449
                     /gene="sufD"
                     /locus_tag="VGP27_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV7855022.1"
                     /translation="MAQNLTTAVESQNKGELFTSFDVDAFEVPGGRDEIWRFTPLKRL
                     RGLHDGSAPATGSATIEVADCPGVTVETVRRGDDRLGQAGVPADRVAAQAFSSFNSAT
                     IVTIPKDVVCEQPIEIDVKGPGEGATAYGHLQVRVGELGEAVVVIDQNGSGTYADNIE
                     FVVADAARLTVVSIADWADDAVHVSSHHAVLGKDAVLRHVAVTLGGDVVRLTARTRFD
                     APGGDAELLGLYFADDGQHFESRLLVDHAQPNCRSHVTYKGALQGDPDSSKPDAHTVW
                     IGDVLIRAAATGTDTFETNRNLVLTDGARADSVPNLEIETGEIAGAGHASATGRFDDE
                     QLFYLQARGIPEDQARRLVVRGFFGELIQKIAVPAVRERLTEAIENELAITESRKTTS
                     "
     gene            2446..3237
                     /gene="sufC"
                     /locus_tag="VGP27_27210"
     CDS             2446..3237
                     /gene="sufC"
                     /locus_tag="VGP27_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV7855023.1"
                     /translation="MTTLEVKDLHVSVVSKEDGTDIPILKGVDLTVKSGETHALMGPN
                     GSGKSTLSYAIAGHPKYTVTSGSITLDGEDVLAMSIDERARAGLFLAMQYPIEVPGVS
                     MSNFLRTAATAVRGEAPKLRHWVKEVKGAMEGLDIDPSFSERSVNEGFSGGEKKRHEI
                     LQLELLKPKIAILDETDSGLDVDALRVVSDGVNRYAEDAHGGLLLITHYTRILRYIQP
                     QFVHVFIGGRIVESGGRELADELDANGYERFSAASGRDPHQAVGA"
     gene            3239..>3360
                     /locus_tag="VGP27_27215"
     CDS             3239..>3360
                     /locus_tag="VGP27_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855024.1"
                     /translation="MTTSAQTLDLTKIRADFPILSRVMRGGNQLAYLDSGATSQR"
BASE COUNT          670 a         1056 c         1084 g          550 t
ORIGIN      
        1 tgcgcacatt cgacaagaag ccgatgccga actggggctc caacctcgaa ggcatccact
       61 tcgacaacat caagtacttc gtgcgctcca gtgagaagca ggcagcaacg tgggacgacc
      121 tgcccgcgga catcaagaac acgtacgaca agctcggcat ccccgaggcc gagaagcaac
      181 gtctggtgtc cggtgtagcg gctcagtacg aatccgaggt tgtttaccac tcgatccggg
      241 aagaccttga ggcacaaggg gttatcttcc tggacaccga caccgcactg aaggaacacc
      301 cggagatctt caagcagtac ttcggcaccg tgatcccggc cggggacaac aagttctcag
      361 cgctgaatac cgctgtgtgg tcgggcggtt cgttcatcta cgtgccaacg ggcgttcacg
      421 tcgacattcc gctgcaggcc tacttcagga tcaacaccga gaacatgggt cagttcgagc
      481 ggacgctgat catcgtcgac gaggacgcct acgtgcatta cgtcgagggc tgcaccgcgc
      541 cgatctacaa gagcgactcg ctgcactccg cggtcgtcga gatcatcgtg aagcccggtg
      601 gccgttgccg ttacacgacc atccagaact ggtcgaacaa cgtctacaac ctggtcacca
      661 agcgggcccg cgccgaggcg ggcgccacca tggagtgggt tgacggcaac atcggttcca
      721 aggtcacgat gaagtacccg gccgtgtgga tgaccggcga gcacgccaag ggcgaggtgc
      781 tgtcggtcgc gttcgcaggc gagggccagc accaggacac cggcgccaag atgctgcacc
      841 tggcgccgaa cacgtcgagc aacatcgtgt caaagtcggt ggcgcgcggc ggcggtcgcg
      901 cgtcctaccg cggtctcgtc caggtgaaca agggcgcgca cggctcgcgg tccagcgtga
      961 aatgcgatgc gctgcttgtc gacacgatca gccgtagcga cacctatccc tacgtcgaca
     1021 tccgcgagga cgacgtcacg atgggtcacg aggccactgt ctcgaaggtc agcgaggacc
     1081 agctgttcta tctgatgagc cgcggcatga ctgaggacga ggcgatggcg atggtggtgc
     1141 gcggcttcgt cgagccgatc gccaaggaac tgccgatgga gtacgcgctg gagctcaacc
     1201 gcctcatcga gctgcagatg gaaggcgcgg tcggctaggt gggacgagcg aagcgacggg
     1261 gtttgatcta gtggcccaga acctgacaac agccgtcgag agccagaaca agggcgagct
     1321 gttcacttcc tttgacgtcg acgccttcga ggtgccaggc ggtcgcgacg agatctggcg
     1381 cttcacgccg ctcaagcgct tacgtggcct gcacgatggg tccgcgcccg cgaccggtag
     1441 cgcgacaatc gaggtcgccg actgccccgg cgtcaccgtc gaaacggtgc gcaggggaga
     1501 cgatcggctg ggccaggctg gtgtgcccgc cgaccgcgtt gccgcgcagg cattttcgtc
     1561 gttcaacagc gcaaccatcg tcaccatccc gaaggacgtg gtgtgcgagc agccgatcga
     1621 gatcgacgta aagggcccgg gcgagggcgc cacggcctac ggccacctgc aggtccgagt
     1681 cggcgaactc ggtgaggcgg tcgtcgtcat cgatcagaac ggcagtggaa cctacgcgga
     1741 caacatcgag ttcgttgttg ccgacgccgc ccggctgacc gtggtctcga ttgccgactg
     1801 ggccgacgat gcggtgcacg tcagcagcca ccacgccgtg ctgggtaagg acgcggtgct
     1861 caggcatgtc gcagtcactc tcggcggcga cgtggtgcga ttgaccgccc gtacccggtt
     1921 cgacgctccc ggtggggacg cggagctgct cggtctctac ttcgccgacg acggccagca
     1981 cttcgaatca cgcctgctgg tcgaccatgc ccaaccgaat tgccgctcgc acgtcaccta
     2041 caagggtgcg ctgcaaggcg atccggactc gagcaagccc gacgcgcaca ccgtgtggat
     2101 cggtgacgta ctgatccggg cggcggccac cggaacagac accttcgaga cgaaccgcaa
     2161 cctggttctc accgatggag cccgtgccga ctcggtgccg aacctcgaga tcgagacagg
     2221 cgagatcgcc ggtgccggac acgcgagcgc cacaggacgt ttcgacgacg agcagctgtt
     2281 ctatttgcag gcgcgcggga tcccggaaga tcaggcccgc cgcctcgtcg tccgcggctt
     2341 cttcggcgag ctgattcaga agatcgccgt gcccgccgtg cgtgaacgcc tcaccgaagc
     2401 catcgaaaac gaactagcga ttaccgaatc gaggaaaacc acctcatgac cacactggaa
     2461 gtcaaggatc tgcacgtcag cgtcgtgtcc aaagaggacg gcaccgacat cccgatcctc
     2521 aagggcgttg acctcaccgt gaaatcgggg gagacgcacg cactgatggg gcccaacggg
     2581 tccggcaaat cgacactgtc gtatgcgatt gcaggccatc cgaagtacac ggtgacgtcg
     2641 ggttcgatca cgctcgacgg tgaggacgtc cttgcgatga gcatcgacga gcgcgcccgc
     2701 gccggcctgt tcctcgccat gcagtacccg atcgaggtgc cgggtgtgtc gatgtcgaac
     2761 ttcctgcgca ccgcggctac cgccgtgcgg ggcgaggcgc cgaagcttcg gcactgggtc
     2821 aaagaggtca agggcgcgat ggaggggctc gacatcgatc cgtcgttctc cgaacgcagt
     2881 gtcaacgaag gcttctcggg cggtgagaag aagcggcacg agatcctgca actcgaactg
     2941 ctcaagccga agatcgcgat cttggacgag accgattcgg gcctggacgt cgacgcgctg
     3001 cgagtcgtca gcgacggcgt caaccgctac gccgaagatg cgcacggtgg tttgctgctt
     3061 atcacgcact acacccgcat ccttaggtac atccagccgc agttcgttca cgtcttcatc
     3121 ggcggccgca tcgtggagtc gggcggccgc gagttggccg atgagctgga cgccaatggc
     3181 tacgaacgat tctctgccgc aagcgggagg gacccccacc aggcggttgg agcctgacat
     3241 gacgacgtcg gctcagactt tggatctcac caagatcagg gcggactttc ccatcctcag
     3301 ccgggtgatg cgcggcggga accagctggc ctatctcgac tccggcgcca cgtcgcagcg
//
LOCUS       DASYLX010001148         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_752530,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001148 DASYLX010000000
VERSION     DASYLX010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752530"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VGP27_27220"
     CDS             <1..540
                     /locus_tag="VGP27_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV7855025.1"
                     /translation="SAAAALTADGPLEYLEQPCATVPELAELRRLVDVPIAADESIRK
                     AEDPLHVVRSGAADIAVLKVAPLGGVARMLAIARQIDIPVVVSSALDSAVGIGRGLLA
                     AGAMPELRHACGLGTGGLFVDDVAEPMTPVDGCLPVATVTPDPARLQALAAPPDRRQW
                     WIDRIMACYESGRADLMAE"
     gene            complement(511..759)
                     /locus_tag="VGP27_27225"
     CDS             complement(511..759)
                     /locus_tag="VGP27_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855026.1"
                     /translation="MAALRRLLSRKVSIEAMLEFAMWIAIPYLVVGIVWTFLNADDVA
                     RIEDRLHTRMPAGAELAAFGATTALWPFLLFGHEVCAP"
     gene            complement(762..1500)
                     /locus_tag="VGP27_27230"
                     /pseudo
     CDS             complement(762..1500)
                     /locus_tag="VGP27_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
     gene            complement(1497..1721)
                     /locus_tag="VGP27_27235"
     CDS             complement(1497..1721)
                     /locus_tag="VGP27_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855027.1"
                     /translation="MLATLQKLLRLEMTIAEWIGTAVLAATAYLVIGVMWSATHTADL
                     GGLTGTDRTMSILGSVVSWPELLFSDVCLA"
BASE COUNT          314 a          579 c          576 g          283 t
ORIGIN      
        1 tccgcggccg cggcgttgac cgctgacgga ccgctggagt acctcgagca gccctgcgca
       61 acggtgccgg aactggccga actgcgtcgg ctcgtcgacg tgccgatcgc cgccgacgag
      121 agcatccgca aggccgagga ccccttacac gtcgtgcgct cgggcgccgc cgacatcgcc
      181 gtgctgaagg tcgcgccatt gggtggcgtc gccaggatgc tggccatcgc ccggcagatc
      241 gacatcccgg tggtggtgtc gagcgcgctc gactcggcgg tcggcatcgg ccgtggcctg
      301 ctggccgcgg gtgcaatgcc ggagcttcgg cacgcctgcg ggctgggcac cggcgggttg
      361 tttgtcgacg acgtcgccga accgatgacg ccagtcgacg gctgtctgcc cgtcgcgacc
      421 gtcactcccg acccggctcg gctgcaggcg ttggcggcac cgcccgatcg acgtcagtgg
      481 tggatcgacc gaatcatggc ctgctatgaa tcagggcgcg cagacctcat ggccgaatag
      541 caggaatggc cacagcgcgg tcgtcgctcc gaacgccgcg agttcggcgc ccgcgggcat
      601 gcgtgtgtgt agccggtctt cgatccgcgc gacgtcgtcg gcgttgagga acgtccagac
      661 gatgccgacg acgaggtagg ggatcgcgat ccacatggcg aactcgagca tcgcctcgat
      721 gctcaccttc cgactcaaca gccgacgcag ggcggccacc gtcacaccag gctgctgtgt
      781 gcgcggaccc gcctgcgcac gtcggccaga acgaggaagc tgccgccctg gcggatgaac
      841 accggcagaa accgattcgc gaaagcgacg agaaggaaag gccattcgaa gcggcgctgc
      901 ttgcccttac tccagcgcag acccaacgcg tccctgaaga ctggagcgag gaagccgatc
      961 gtgaggaact tcatcagtgg ccgaaagggc agccccacaa cagggttgat catccgcagg
     1021 ttgatcagat cgagcaggta ctcgcggacg ggagcgtcga tgacgacctg cttgcacgcg
     1081 ttgtcccagt aggcgtcgaa ctcggtacgc gtcgccggcc attgatcctc ggtgacctgt
     1141 agggtcgtgc ccaacgtcca cgatgagcgg tagaactgct cggcctggtc ggccgtcatc
     1201 tcgccgcgca gcaacgtgta ggtgtcctcg agaccgacga aaaggcatgc ggccacccac
     1261 atttgcagct cacggtcgaa cccgttgtac gtcaccgggc tctcggcggt ggagatcacc
     1321 tggcgagtcg accttgctct ccacgacgcc gtatcccaca ccgggcgatg ccaattgcat
     1381 caccacgttg gccgccgcgc cggcgaacga ccagaagtcc atggcctcct tcacagagac
     1441 ctcggcgtcg ggatcccagc gcgcggtccg cctggtgacc tcgatacggg tgccggtcat
     1501 gccaagcaga catccgagaa cagcaactcc ggccagctga cgaccgatcc gaggatcgac
     1561 attgtgcgat cggttccggt cagtccgccg aggtcggcgg tatgggtggc ggaccacatc
     1621 acgccgatca ccaggtaggc agtcgcggcg aggacggccg tcccgatcca ctcggcaatc
     1681 gtcatttcga ggcgtagaag cttctgcagc gtggcaagca cgcgtctatg ttaatgatca
     1741 ttccgcgctg ct
//
LOCUS       DASYLX010001149         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1957018,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001149 DASYLX010000000
VERSION     DASYLX010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957018"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VGP27_27240"
     CDS             complement(<1..1343)
                     /locus_tag="VGP27_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7855028.1"
                     /translation="MAEPVLTVTDLRVRIGRREIVRGVSFVVERESTLGIVGESGSGK
                     SMTVLAATGLLDAPGAVVSGSSTLAAAGDATATQLVGASARVLRDVHGGRIGFVFQDP
                     GTSLNPLLTVERQITESLQTHRNMTRRQATDRAIELIEAVGLPEPRTRLRSYPHQLSG
                     GQRQRVMVAIALACDPELLIADEPTTSLDVTTQSQIIDLIRDLQKDFGTAVVWISHDL
                     GVIGQVADDVTVLQDGQAVEQAPILDVFDNPQQPYTRELLKARPLIGVSGPPPADAEA
                     PVLLAVDGLDVRFPVSTPTGRSTVHAVKDVSFQIRRATTLGLVGESGSGKSTVAATLT
                     GLVEPDAGSASLDGIDVFGVRGRAEKGLRRRISLVFQDPFSSLNPRTRAGTAIEEPLR
                     VHGLAKGKQARQKRVAELLELVGLPASFAARYPHELSGGQRQRVSIARALATEPDL"
     gene            complement(1336..>1561)
                     /locus_tag="VGP27_27245"
     CDS             complement(1336..>1561)
                     /locus_tag="VGP27_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7855029.1"
                     /translation="ALSFLGLGIQPPQPSLGRMIFDSQGFVTLAWWMAVFPGAAIVVT
                     VLAFNLLGDGLRDVLDPKQRTMIEARRHSG"
BASE COUNT          263 a          520 c          514 g          264 t
ORIGIN      
        1 aggtcgggtt cggtggcaag ggcgcgggcg atgctgaccc gttgtcgttg gccgccggac
       61 agctcgtgcg ggtaacgcgc cgcgaacgac gcaggcaggc cgacgagttc gagcagttcg
      121 gccactcgct tctggcgcgc ctgctttccc ttcgcgaggc cgtgtactcg caacggctct
      181 tcgatcgccg ttcccgcgcg ggtgcggggg ttcagcgacg agaacggatc ctggaacacg
      241 aggctgatgc gcctgcgcag acccttctcc gcgcggccgc gtacgccgaa cacgtcgatt
      301 ccgtccagtg aggcactgcc ggcatccggt tcgacgagtc cggtgagcgt cgccgccacc
      361 gtcgacttgc ccgaaccgga ttcgccgacg agtcccagcg tggttgcgcg ccgaatctgg
      421 aaactcacgt ccttcaccgc atgaacggtc gaccttccgg tcggcgtgct caccgggaac
      481 cggacgtcca atccgtccac ggcgagcaac accggcgctt cggcgtcggc aggcggcggg
      541 cccgacaccc cgatgagggg tcgggctttg agcagttccc tggtgtaggg ctgttgtggg
      601 ttgtcgaaca catcgagaat cggcgcctgc tcgacggctt ggccgtcctg cagaacggtg
      661 acgtcgtcgg ccacctggcc gatgaccccg aggtcgtggc tgatccacac caccgcggtg
      721 ccgaagtctt tctgcagatc gcggatcaag tcgatgatct gcgactgtgt cgtgacgtca
      781 agcgatgtgg tcggctcatc ggcgatcagc agttcgggat cacacgccaa cgcgatcgcg
      841 accatcacgc gctgtcgctg cccacccgac agctgatgcg gataggacct cagccgcgtc
      901 cgaggctccg gcaggcctac cgcctcgatc agctcgattg cgcggtccgt ggcctgccgc
      961 ctggtcatgt ttcggtgtgt ctgaagggat tcggtgatct ggcgctcgac tgtgagcaga
     1021 gggttgagcg aggttccggg atcctggaat acgaatccga tgcgaccgcc gtgaacgtcg
     1081 cgcagcacac gggcagaggc accgacgagc tgtgtcgctg tcgcgtcgcc cgccgctgcc
     1141 agcgtgctgg aaccgctcac gacggcgccg ggtgcgtcga gcagtcccgt cgctgcgagc
     1201 acggtcatcg acttacccga accggactcg ccgacgatgc ccagcgtgga ctcgcgctcg
     1261 acaacgaagg acactccccg gacgatctcg cgcctgccga ttcgcacgcg taggtcggtc
     1321 acggtcaata caggttcagc cactgtgcct ccgcgcctcg atcatggtgc gctgcttggg
     1381 atccagcaca tcgcgcagcc cgtcgccaag caggttgaac gccagcacag tgacgacgat
     1441 ggcggcgcct ggaaacaccg ccatccacca cgcgagtgtc acgaacccct gagagtcgaa
     1501 gatcatccgg cccaacgacg gctgcggagg ctggatgcca agaccgagga atgacagcgc
     1561 c
//
LOCUS       DASYLX010001150         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1298686,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001150 DASYLX010000000
VERSION     DASYLX010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298686"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGP27_27250"
     CDS             <1..367
                     /locus_tag="VGP27_27250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7855030.1"
                     /translation="AIEFVEADVTALQFADNSFDLCVTYNGLHCLPDPRAALGELTRV
                     LKPGGTLRGTSCVAGRGPRKDALIAMLRRAGVFGNAPRAGDIEAWLREFGLDVLTLER
                     SGAVEFFEARLPSGEQTDR"
     gene            364..534
                     /locus_tag="VGP27_27255"
                     /pseudo
     CDS             364..534
                     /locus_tag="VGP27_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxybiphenyl 1,2-dioxygenase"
     gene            671..1609
                     /locus_tag="VGP27_27260"
     CDS             671..1609
                     /locus_tag="VGP27_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7855031.1"
                     /translation="MPVSILRTVDAWWVQTPTGAARITTAAATTRQLLDDRAAIDAAD
                     RRTDTVPLDSLQLLSPITAPCRVVAQLTNYASHVKDAGRDPANTPLAFFRKASGSISG
                     PLDDIVKPQHVRLLDYEVEIGLVIGRDMPVGTTITEANLSDYIAGLVVANDVSARDVQ
                     LTKTQFYEAKSYPTFTPVGPALVLLSADELKRFGDLRLRLQVNGDIRQDMLVDGDIIY
                     PPVQALQALSRFQRLDAGDLVLTGTPVGTAISAPPRPIEIIGSLLPDQLKWKAFFSRQ
                     AKNPEYLRPGDLIEAAVATDDGAIDLGVQRTAVKYS"
     gene            1625..>2268
                     /locus_tag="VGP27_27265"
     CDS             1625..>2268
                     /locus_tag="VGP27_27265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7855032.1"
                     /translation="MATRARDLVLDTLTVEQDDRVLTVRFCDPPYNYMTARMQKDLDT
                     LTAVVDFDVSVGAVVLTGGVPNRYITHFDIAEILAAAQHVRPPLSGQALLNLLRNIDF
                     DAEPGGERALERTPLSQFLSVPRFNQVVLRIMRSPAVYIAAIGGPCGGGGLEMSVCFD
                     VRLAADDESVGFILPELLIGLTTTFGGQRLAQLIGPARALEMMLEGRMYSAQEA"
BASE COUNT          431 a          739 c          687 g          411 t
ORIGIN      
        1 cgcgatcgaa ttcgtcgaag ccgacgtgac cgcacttcaa ttcgccgaca acagcttcga
       61 tctctgcgtg acctataacg ggctccactg tctgccggac ccgcgtgcgg cgctgggaga
      121 gctcactcgc gttctcaagc ccggagggac gctgcggggg acgtcatgtg ttgcgggtcg
      181 aggcccgcgc aaggatgctc tgatcgcgat gttgcggcgc gccggggtgt ttggaaacgc
      241 tccacgcgca ggcgatatcg aggcgtggtt gagggaattc ggcctcgatg tcctgacact
      301 ggagcgcagc ggagcggtcg agtttttcga ggcgcgattg ccatcgggag agcagacaga
      361 ccgatgagcg gccatggtgt cggcgcgcac aacgatctgc acagtgatca gggcgctcgc
      421 aaggacgagc atccggggcg ctcacgtggg ccgatcatca aggtgcacga catcgcgtgg
      481 ttggagttcg agaagccgga tctggcacgg gctgaggcgt tttcccaagc gttcgcgccc
      541 ggccgcgaat ctctgcgcga acttcgcaag gtgatcagcg cgttgcgcac cgacaacgaa
      601 ttcgatctca cgagcctccg cggcttgctc gccgtggcca actcctaata ccacgattta
      661 aggacccaag atgcccgtct cgatcctgcg caccgtcgac gcctggtggg tgcagactcc
      721 aaccggtgcc gctcgaatca ccaccgcggc cgccaccacc cgccagctgc tggacgatcg
      781 cgccgccatc gacgccgccg accgtcgcac agacaccgtg ccgctggaca gcctgcaact
      841 gctgtccccg atcaccgccc cctgccgggt ggtggcacaa ctgaccaact acgcctccca
      901 tgtcaaagac gccgggcggg acccagccaa cactccactc gccttcttcc gcaaggcatc
      961 cggctccatc agtggcccac tggacgacat cgtcaaaccc caacacgtgc ggctgctcga
     1021 ctacgaggtg gagatcggtc tggtcatcgg ccgcgatatg cctgtgggca ccacgatcac
     1081 cgaagccaac ctctcggact acatcgccgg cctggtagtg gccaacgacg tatccgcgcg
     1141 cgatgtgcag ttgacgaaaa cgcagttcta cgaagcgaag tcgtatccga cattcactcc
     1201 ggtcggcccc gcactggtgc tgctgagcgc cgacgagctc aagcgcttcg gtgacttgcg
     1261 gctgcggctc caggtgaacg gtgatatccg acaagacatg ctcgtcgacg gcgacatcat
     1321 ctacccaccc gtgcaggcac tgcaggccct gtcgcgattt cagcgtctcg acgccggcga
     1381 tctggtatta accggtaccc cggtcggcac ggcaatcagc gcaccgccca gaccaatcga
     1441 gatcattgga tcgctgcttc cggaccagct gaagtggaag gccttcttct ccaggcaagc
     1501 caagaatccg gaatatctac gccccggcga cctcatcgag gccgccgtgg cgaccgacga
     1561 cggagcaatc gacctcggtg tgcaacgcac cgccgtgaag tacagctgag cggggacggc
     1621 tgatatggcg acccgcgcac gcgaccttgt tctggacacc ctgacggtcg agcaggatga
     1681 tcgagtactc actgtccggt tctgcgatcc accgtataac tacatgacgg cgcggatgca
     1741 gaaggacctt gacactctca ccgcagtcgt cgacttcgac gtcagtgtcg gtgcggtcgt
     1801 tctcaccgga ggcgtaccga accgctacat cacccatttc gacatcgccg agattctcgc
     1861 cgccgcccag catgtcagac caccgttatc cggtcaggca ttgttgaacc tcctgcggaa
     1921 cattgacttc gatgccgaac cgggcggtga acgggccctg gaacgaactc ccctcagcca
     1981 gtttttgagt gtcccgcggt tcaatcaggt cgtgctgcgc atcatgcgat cgcctgcggt
     2041 ttacatcgct gcgatcggag gaccgtgcgg cggtggagga ttggagatgt cggtctgctt
     2101 cgacgtccgc ttggcggcag atgacgaatc cgttggcttc atacttcccg agctcctcat
     2161 cggattgacc accacattcg ggggtcagcg actggctcag ctgatcgggc ctgccagggc
     2221 gctggagatg atgctggagg gccggatgta ctccgcccag gaggcaca
//
LOCUS       DASYLX010001151         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3825578,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001151 DASYLX010000000
VERSION     DASYLX010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825578"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGP27_27270"
     CDS             complement(<1..204)
                     /locus_tag="VGP27_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7855033.1"
                     /translation="MAEFIYTMRKVRKAHGDKVILDDVTLNFLPGAKIGVVGPNGAGK
                     SSVLRIMAGLDQANNGDAYLQPGA"
     gene            complement(300..776)
                     /locus_tag="VGP27_27275"
     CDS             complement(300..776)
                     /locus_tag="VGP27_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV7855034.1"
                     /translation="MFETPISVVGTIITDPILRRVGDQEVYKFRVASNSRRRTAEGNW
                     EPGNSLYVSVNCWGKRLVKGVAGTLGKGDPVIVVGFIHTSEYEDREGVRRSSLEVRAT
                     AVGPDLARSTTSIDRPKVNSVTSDDAADTTDGQLGDEAETTESDEAVGEKALPLSA"
     gene            932..1321
                     /locus_tag="VGP27_27280"
                     /pseudo
     CDS             932..1321
                     /locus_tag="VGP27_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099159.1"
                     /note="catalyzes the hydrolysis of allophanate;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
     gene            complement(1391..>1765)
                     /locus_tag="VGP27_27285"
     CDS             complement(1391..>1765)
                     /locus_tag="VGP27_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7855035.1"
                     /translation="EQVACFVNAAAHLVPGGSFVIEVGVPALRLLPPGQRAVPFDVGD
                     RYWAYDLYDCATQSMSSNYVDDADGNVRLWSIPFRYVWPAELDLMARIAGLVLVERWQ
                     DWDGTPFSHESTRHVSVWRKPT"
BASE COUNT          274 a          602 c          560 g          329 t
ORIGIN      
        1 cgcgcccggc tgcaggtagg catcgccgtt gttggcctgg tccaggccgg ccatgatccg
       61 caggacgctc gactttcccg ccccgttcgg accgacgacg ccgatcttcg cgcccggcag
      121 gaagttcaag gtgacgtcgt cgaggatgac cttgtcgccg tgcgccttgc ggaccttccg
      181 catcgtgtag atgaattcgg ccatgccgtt gtaatgctcc tcttgtctcg gtattgctcg
      241 cggaccatcg taggcgttga ccatggccgt cccgacctcg cgtcagaggc cctgcctgct
      301 cacgcagaca acgggagagc cttctcaccg acggcctcgt ccgactcggt cgtctcggct
      361 tcgtcgccga gctggccgtc cgtggtgtcc gcagcgtcgt cgctggtcac gctattgacc
      421 ttcggacgat cgatgctcgt ggtgcttcgc gccagatccg gcccgaccgc ggtggcccgc
      481 acttccagcg acgaacgtcg aacgccttcg cggtcttcgt actcgctggt gtggataaat
      541 ccaacaacga tgaccgggtc acccttcccg agcgtgcctg cgacgccctt cacgaggcgc
      601 ttgccccagc agttgaccga cacgtacagg gagttgcccg gctcccagtt gccctcggcg
      661 gtgcgccggc gcgaattgct ggccacccgg aacttgtaga cctcttgatc gccgaccctc
      721 cgcaatatcg gatccgtgat gatcgtgccg accacgctga ttggtgtctc gaacatttct
      781 cgtcctttcg attcatgtgc cttgcgccgt acctgaccca ttgatccggt cgacgccgac
      841 gcgtcgcggt ggcgcggcgt gttctcggcg gccggctgtg gacgaatcgc ggactgtgca
      901 cagccgccgt aggttgacaa ccatggcgga caacctggag ttggtggtgt tcggagtgca
      961 cctgcgcggc ggaccgctgg cccatcagct caccgatctc ggcgcgcggt gggcaggcga
     1021 gctgacgacg gcgccgcgct accggatgac agttctgccg acgacgcccg ccaaacccgc
     1081 aatcacccga gtcgcggacg gcgcggcagg cgcctcgctg atgggccacc ggtggctgct
     1141 ctcccccgcc gcgctcggca tgtttctcga tgcgttgccc acgcccatgc agctcggcaa
     1201 ggtcgaattc gacgacggca catggcgaac cgccttcagc tgtgacggct cggcggcgac
     1261 cgaccctgac atcagctcgt tcgggagttg gccggcagcg atcgccgcag gcgcggtcta
     1321 gagctcgcga aacgtaattc cagcaggccc ttactcgcgc acttcttctg ccggaattcc
     1381 gtttcgcggt ctaggtcggc ttccgccaca ccgagacgtg gcgggtgctc tcgtggctga
     1441 acggcgtgcc gtcccagtct tgccatcgct cgacgagcac cagaccggcg atgcgtgcca
     1501 tcaggtcgag ttcggccggc cacacgtagc ggaacggaat ggaccagagc cggacgttgc
     1561 cgtcggcgtc gtcgacgtaa ttggagctca tcgactgcgt cgcgcagtcg tagaggtcgt
     1621 aagcccaata cctgtcgccg acgtcgaacg gaacggcgcg ctggcccggc ggcagaagcc
     1681 gcagggcggg aacaccgacc tcgatcacga aactgccacc cggcaccaga tgtgcggcgg
     1741 cgttgacgaa gcaggcgacc tgttc
//
LOCUS       DASYLX010001152         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2615852,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001152 DASYLX010000000
VERSION     DASYLX010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615852"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGP27_27290"
     CDS             complement(<1..601)
                     /locus_tag="VGP27_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7855036.1"
                     /translation="MSTFTETMYINALRSPKGMVTGEPHAPVRHTWAQVHQRARRVAG
                     GLARSGIGHGDAVAVLAGAPVEIAPAAQGIWMRGASLTMLHQPTPRTDLTRWAAETTG
                     IIDMIRAKAVVVSDPFMPAAPVLAGLGLRVLTFDELISSTPIDPVHTDDDDLALMQLT
                     SGSTGSPKAVQISHRNFVANAEAMFTGAEVDPDTDVIVSW"
     gene            complement(923..1516)
                     /locus_tag="VGP27_27295"
     CDS             complement(923..1516)
                     /locus_tag="VGP27_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7855037.1"
                     /translation="MPRAKQRTPELRDRVLDVAITTLGEEGIARFTTRRVAERAGTSV
                     PAVYELFHDKAGLVRAMFFEGFRLLGAELARIPETDDTLGDLERLVPVFRLFCRAYPK
                     LAQVMFSRPFADFEPGTDELAAGAAVREIFVGRIQRCVDKGLLGGDPTDIAHVLLALA
                     QGLAVQEAGRWLGTSTEIERRWKVGVTSVLDGFGWAN"
     gene            complement(1526..2227)
                     /locus_tag="VGP27_27300"
     CDS             complement(1526..2227)
                     /locus_tag="VGP27_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7855038.1"
                     /translation="MTNPVNYELNESVATIALDDGKVNVLGPTMQAAINEALDRAEKD
                     DAKAVVLAGNQRVFSGGFDLSVFQSGDAQAALAMLAGGFELSIRCLTFPKPVIMAATG
                     PAIAMGSFLLLSGDHRIGAPKSRCQAIEVAIGMTIPISAIEIMRMRLTPAAFARGTSM
                     ASTFVGDEAIAAGWLDEIVDADKVLARAKEVAVEAAATLHAGAHLATKLKARESALKA
                     IRAGIDGLPAEFTAS"
BASE COUNT          392 a          796 c          753 g          427 t
ORIGIN      
        1 gccagctgac gatcacatcg gtatccgggt cgacttccgc cccggtgaac atcgcctccg
       61 cattggccac gaagttgcga tggctgatct ggaccgcctt gggagagccc gttgacccgg
      121 acgtcaactg catcagcgcc agatcgtcgt cgtcggtgtg aacggggtcg atcggcgtac
      181 tcgagatcag ttcgtcgaag gtgagcactc tcaatccgag gccggccagc acaggcgccg
      241 caggcatgaa ggggtcggag acgacgacgg ccttcgcgcg gatcatgtcg atgatgcccg
      301 tggtctctgc tgcccaccga gtgaggtccg ttcgcggagt cggttggtgc agcattgtca
      361 agctcgcccc acgcatccaa ataccctgcg cggcaggggc tatttcaacc ggagcaccgg
      421 ctaataccgc caccgcgtct ccatgaccaa tgccggaccg ggcgagtcca ccggcaacgc
      481 gcctggcccg ctggtgtacc tgcgcccacg tgtgccggac cggcgcgtgg ggctcacccg
      541 tgaccatgcc cttggggctg cgcagggcgt tgatgtacat ggtttcggtg aatgtgctca
      601 cgacaattcc tttggctccg gcggttcgac gcgcggtggt gcgcgtagtg aaccggcgtg
      661 cagacgttga tgagccgctg aggtgcggcc atcgtccatt cttttccagg tggatgacta
      721 ctgcgggagg actcggcccc ttcttgttga cctcagatga atccctgtgc tccggcagga
      781 gcgcggtggg gcagagttaa ggacttcgga cgttctgact gtacagacct acgcgtcaat
      841 ggaccgcgca ccgcgccgag acgagtttca gcgcgcaaag tcgttttgtc aacagcaatc
      901 tcgttcgggt accaaattga cgtcaattcg cccagccgaa cccgtcgagg actgacgtca
      961 ccccgacctt ccatctgcgc tcgatctcgg tcgacgtgcc cagccaccgg cccgcctcct
     1021 gcaccgccaa accctgtgcg agagcgagta atacgtgcgc gatatccgtc gggtcgccgc
     1081 ccagcaggcc cttgtcgacg caccgctgaa tgcggccgac gaagatctcg cggacggccg
     1141 cacctgcagc cagttcgtcg gtgcccggct cgaagtccgc gaacggccgc gagaacatca
     1201 cctgcgccag tttcggatat gcgcggcaga acagccggaa caccggcacc agccgctcga
     1261 gatccccgag cgtgtcgtct gtctcgggaa ttctggccag ctcggcgccc agcagccgga
     1321 aaccttcgaa gaacatcgcc cgcaccaggc cggccttgtc gtggaacagc tcgtataccg
     1381 cgggcaccga cgtgccggcc cgctcggcca cccgtcgtgt ggtgaaccgc gcgatgccct
     1441 cctcgccgag cgtggtgatc gccacgtcga gcacccgatc cctgagctcg ggcgttcgtt
     1501 gcttcgccct gggcatcgca gtgacctagc tggcggtgaa ttctgcaggc aggccgtcga
     1561 ttccggcgcg aatcgctttc agcgcggatt cgcgtgcctt cagcttggtc gccagatgcg
     1621 cgcctgcatg cagcgtcgcg gcggcctcga ccgccacctc cttcgcgcgc gccagcacct
     1681 tgtccgcgtc gacgatctcg tcgagccagc cggcggcgat cgcctcgtcg ccgacgaatg
     1741 tcgacgccat cgacgtgccc cgcgcaaacg cggcaggcgt cagccgcatc cgcatgattt
     1801 cgatcgcgga gatcggaatg gtcattccga tggccacctc gatggcctga cagcgcgact
     1861 tcggggcgcc gatgcggtga tcgccgctca gcagcagaaa cgagcccatc gcgatggccg
     1921 ggcccgtcgc ggccatgatg accggcttcg ggaaggtcag gcatcggatg gacaattcga
     1981 agccgcccgc cagcatcgcc agggccgcct gcgcgtcgcc ggactgaaag accgaaaggt
     2041 cgaatccgcc gctgaacacg cgctgattgc ctgccagcac gactgccttg gcatcgtcct
     2101 tctcggcgcg gtccagcgcc tcgttgatgg cggcctgcat cgtcggcccc agcacgttga
     2161 ccttgccgtc gtcgagcgcg atcgtcgcga cggactcgtt gagctcgtag ttcaccgggt
     2221 tggtcatcgg ggctcccttg acgtggggga tcgtttcaga tcgatgttac gaaagccaag
     2281 tgccgaatgt cggcgcgtca gccgcccagg ccgcggcgat gcctgccgtt gatcacccgg
     2341 gtctgggcat cgtcgtcgtc atagggcg
//
LOCUS       DASYLX010001153         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1191427,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001153 DASYLX010000000
VERSION     DASYLX010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191427"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGP27_27305"
     CDS             <1..200
                     /locus_tag="VGP27_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855039.1"
                     /translation="LIAIAGPPHNLTVTDNAGQVLSSASLARPPTKPPPTVPPCPGSS
                     GERADWWWYEPFQPQPPPTNN"
     gene            complement(362..1255)
                     /locus_tag="VGP27_27310"
     CDS             complement(362..1255)
                     /locus_tag="VGP27_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7855040.1"
                     /translation="MTTIDPRNEVREFLSSRRARIRPEQAGLPAYGGNRRVKGLRREE
                     VAMLAGVSVDYYVRMERGNLASASDSVLDALASALQLDEAEREHLYALARESGAGGSR
                     RKRRTPGTVRPALQQVLDAITDAPAWIRNGRHDVLAMNQLARALYAPVLADPRRPANT
                     ARFVYLNREVAEQFFVDYDKIARDAAAMLRLEAGQNPHDEDLIALVGELSTRSELFRK
                     QWASQDVRFHRSGRKRLRHPAVGQLDLDFETMQIPSEPGLRLNIYTAPANTPTADGLK
                     LLASWAASQDNLATERAAAKS"
     gene            1357..2244
                     /locus_tag="VGP27_27315"
     CDS             1357..2244
                     /locus_tag="VGP27_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7855041.1"
                     /translation="MSIPTFTLKDGVEIPVLGFGVFQTAPEETIAAVEAALETGYRHI
                     DTAAVYGNEREVGEAIRRSGLARDDIFIETKVWITDFGYDETLHAYDKAAGKLGVEQI
                     DLLILHQALPGEFELTIAAYKALEKLYADGKVRAIGVSNFMPRHLDRLLAETEGVPAV
                     NQIEVHPYFRQSELLAFDEAHGILNQAWAPIGGITFYREGPHTSTLENPVIGEIAAAH
                     SKTPAQVMLRWHIQQGRQVIPKSVTPSRIAENFDVFDFDLSAEQLAAIDALDTGVRGG
                     PEPEAITRENYGMEIPEAG"
     gene            2285..3274
                     /locus_tag="VGP27_27320"
     CDS             2285..3274
                     /locus_tag="VGP27_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7855042.1"
                     /translation="MHTRTLGQGLRVSAVGLGAMGMSQSYGPNPGDRDDMIGVLRYAV
                     EEAGVTFFDTAEVYGPYVNEELVGEALAPFRDEVVIATKFGWNIVDGKMVGTDSRPEQ
                     IRRVADASLQRLQTDVIDLFYQHRVDPNVPIEDVAGTVAELVQAGKVRHFGLSEAGAA
                     TIRRAHAVHPVTAVQSEYSLWTRDPEPEVLPTCAQLGIGFVPFSPLGKGFLTGTVTGT
                     TELAAGDVRTTIPRFAAGNIGANEALVSEVRDLAQAKDCTPGQIALAWLLAQQPWIVP
                     IPGTRRRERVAENAGATQVALSADEVAFLDALAARVGVHGDRYNTAGMGMVGL"
     gene            3472..3885
                     /locus_tag="VGP27_27325"
     CDS             3472..3885
                     /locus_tag="VGP27_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7855043.1"
                     /translation="MQKLQKQPSTKGPAEMFTGEVYFDVVYRGEEPSRMRVNVVRFAP
                     CARTAWHTHAMGQTLHVTDGVGLVQSRGGDVVVMHPGDTVYTPPGEWHWHGAAPEHFM
                     THLAMWEGPDPDSGKTETEWGEHVTDDEYQDRREE"
     gene            4149..5126
                     /locus_tag="VGP27_27330"
     CDS             4149..5126
                     /locus_tag="VGP27_27330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7855044.1"
                     /translation="MKHAKLRDLDVSRIGLGAMGMSFGYTGAGTDDAESIRTIHRALD
                     LGVTFIDTAEIYGPYTNEELVGQALKGRRDQVVVATKFGMVSHTGGARGNLDSSPANI
                     RTAVEGSLRRLGTDYIDLYYQHRVDPNTPIEETVAAMAELVTEGKVRHLGLSEAGVTT
                     IRRAHAVHPITALQSEYSLWTRDPEPAVLPVLRELGIGFVPYSPLGHGFLTGAVRSMD
                     DLDDHDWRKTSPRFMGENFDRNLDIADEVQAIAAEAGATPAQVAIAWLLTKGDDIAPI
                     PGTKRVSRLEENVAADGIELSAEQLEKLNNLTPPAGDHHNEAQMAMIDR"
     gene            5158..5774
                     /locus_tag="VGP27_27335"
                     /pseudo
     CDS             5158..5774
                     /locus_tag="VGP27_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007620627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(5784..>6629)
                     /locus_tag="VGP27_27340"
     CDS             complement(5784..>6629)
                     /locus_tag="VGP27_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7855045.1"
                     /translation="LFKTHAVRDGDEWVINGEKWFTSAGRVADILFVMCTNGMFVVPR
                     TTPGVEIAPEPRNHNHIIYRDVRIPLDHLLGPEDGAKTLAQRRLGGGRIHHAMRTIAQ
                     CKLAFDMMCERALSRESHGKLIGEHQMVQEKIADSYAAIRMLRLLVLETAWKIDNSST
                     KDARTDIATVKFTMAKVLREVSFNALHILGSLGTTDLTPLQAMYASAPTMGLADGADE
                     VHKATVARRVLRDYSPQQHPYWPTEYLPAKREAAWAKFEPKFEADPGLRESAEAYKKY
                     FARRR"
BASE COUNT         1220 a         2212 c         2145 g         1052 t
ORIGIN      
        1 gcctcatcgc catcgccgga cccccacaca acctgaccgt caccgacaac gccggccaag
       61 ttttgagctc tgcatcactg gcgcgaccac cgaccaaacc cccgcccacc gtcccaccgt
      121 gccccggctc cagcggcgaa cgcgccgact ggtggtggta cgaaccattc caaccccaac
      181 cacccccgac gaacaactag agcaaaacgc cgagttctgc cgcagagagt ggcgaccgga
      241 gcgtgcatgc gttccaccct gagctaagac ccgtcctagt cgatcgacag ccccgttttc
      301 ctgggcgggc gtcgtcgtgc ttagtcgcgg tccgtcagct gcgcctcggg gatcaagcgt
      361 cttagctttt ggccgcggcc cgttcggtgg cgaggttgtc ctggctggcc gcccacgaag
      421 ccagcagctt gaggccatcg gcggtcggcg tgttcgcggg cgcggtgtag atgttgagtc
      481 gaaggccagg ctcggaggga atctgcatcg tctcgaagtc gaggtcgagc tgaccaacgg
      541 cggggtgccg cagccgcttt cggccggacc ggtggaaccg gacgtcctgg gacgcccact
      601 gcttccggaa aagctcactg cgcgtagata attcacccac caaggcgatc aagtcctcat
      661 cgtgcggatt ctgaccggcc tcgaggcgaa gcatcgcggc cgcgtcgcgg gcgatcttgt
      721 cgtagtcgac gaagaactgc tcggcaacct cgcggttcag gtacacgaac cgcgcggtgt
      781 tcgcgggccg acgcggatcg gcaagcaccg gcgcgtagag cgcacgggcc aactggttca
      841 tcgccagcac gtcgtggcgc ccgttgcgga tccaggcggg cgcgtcggtg atggcatcga
      901 ggacctgttg tagggccggt cgaacagtcc ccggcgtcct gcgcttacga cggctgcccc
      961 ctgcgccgga ttccctggcg agggcataga ggtgctcgcg ctctgcctcg tcgagttgca
     1021 gtgcggaggc gagggcgtcc agcacgctgt ccgacgcgct cgccaggttg ccgcgctcca
     1081 tgcgtacgta gtagtcgacc gacaccccgg ccagcatcgc gacctcttcg cgccgcagcc
     1141 ccttgactcg gcggttgccg ccgtaggccg ggagccccgc ctgctcaggc ctgatgcgcg
     1201 ctcgtcgcga actcaagaac tcgcggacct cgttgcgcgg gtcgatcgtg gtcatacctt
     1261 cacggtaggc acgcttgccg acagttggga ggtactgcgg gtaccccgtt gatcaggcac
     1321 tcccaggcgc cgtcggcgag gcctagcgtc gatgacatga gcatccccac cttcaccctc
     1381 aaggacggcg tcgagatacc cgtgctgggg ttcggcgtct tccagaccgc accggaagag
     1441 accatcgccg ccgtcgaggc ggcgctggag accggttacc gccacatcga caccgcggcc
     1501 gtgtacggca acgagcgcga ggtcggcgag gcgatccgcc ggtccgggtt ggcccgcgac
     1561 gacatcttca tcgagacgaa ggtctggatt accgacttcg gctacgacga gacgctgcac
     1621 gcttacgaca aggcggcggg caagctcggc gtcgagcaga tcgacctgct gatcctgcac
     1681 caggcgttgc ccggtgagtt cgagctgacc atcgccgcct ataaggcgtt ggagaagctc
     1741 tacgccgacg gcaaggtccg cgcgatcggc gtctcaaact tcatgccgcg ccatctcgac
     1801 cggctgctgg ccgaaactga gggggtgccg gcggtcaacc agatcgaagt ccatccatac
     1861 ttccggcaat ccgaattgct cgcattcgac gaagcacacg gcatcctcaa ccaggcctgg
     1921 gcacctatcg ggggcatcac gttctaccgc gagggcccgc acacgtcgac gctcgagaac
     1981 ccggtaatcg gcgagatcgc cgccgcgcac tccaagacgc cggcgcaggt gatgctgcgg
     2041 tggcacatac agcagggccg ccaggtgatc ccgaagtcgg tgacgccgtc gcggatcgcc
     2101 gagaacttcg acgtcttcga cttcgacctg agcgccgagc aactggccgc catcgacgcg
     2161 ctggacaccg gcgtccgcgg cggtccggag cccgaagcca tcactcgcga gaactacggg
     2221 atggaaatcc cagaagccgg ctgatgagcg cttgcgcgaa gaagaaacca agcgaggagg
     2281 attgatgcac acacgtacgc tcggccaagg acttcgcgtt tcggcggtcg gtctcggcgc
     2341 tatggggatg tcgcagagct acggccccaa tcccggagac cgcgacgaca tgatcggcgt
     2401 tctgcgctac gcagtggaag aggccggggt gacgttcttc gacaccgccg aggtctacgg
     2461 gccctatgtc aacgaggaac tcgtcgggga ggcgttggcg ccgttccgcg acgaggtcgt
     2521 gatcgccacc aagttcggtt ggaacatcgt cgacggcaag atggtcggca ccgactcccg
     2581 cccagagcag atcaggcgag tcgccgacgc gtcgctgcag cggctgcaga ccgacgtcat
     2641 cgatctgttc taccagcatc gggttgaccc gaatgtgccg atcgaagacg tagcaggcac
     2701 ggtcgccgaa ctcgtccaag ccggtaaggt ccggcacttc gggctctcgg aggccggtgc
     2761 cgccaccatc cgccgcgccc acgccgtgca ccccgtcacg gcggtgcaaa gcgagtactc
     2821 gctgtggacc cgggaccctg agccggaagt gctgcccacc tgcgcccaac tcggcatcgg
     2881 gttcgtgccg ttcagtccac tgggcaaggg cttcctcacc ggaactgtca cgggcacaac
     2941 cgagctcgcc gccggtgatg tccggacgac catcccgcgg ttcgcggccg gcaacatcgg
     3001 cgccaacgag gcgctggtga gcgaggtccg cgacctcgcc caggcgaagg actgcacgcc
     3061 tggacagatt gcgctggcgt ggctgctcgc gcagcagccg tggatcgtgc ccatccccgg
     3121 cactcgtcgc cgcgaacgcg tcgccgagaa cgccggcgct acccaggttg cgctctcggc
     3181 tgacgaagtc gccttcctcg acgcgctggc cgcacgcgtc ggcgtccacg gtgacaggta
     3241 caacacggcc ggcatgggga tggtcggcct gtgacgacga gaggctggac cactgaccaa
     3301 ctcgaccgca tcagcgaggc gcaggagatg cacatcgcgg gccgccgccc agacggctca
     3361 gtacgggcag tatccgggcc cggtggcggc catcaccagc ccgtcggcgc gcgccaccac
     3421 gctcaagctc gtgccgcacg ccggcacacc cgaaccaccg aaagagtgac tatgcagaaa
     3481 ctccagaaac agccctccac caaaggaccg gccgagatgt tcaccggcga ggtgtatttc
     3541 gacgtcgtct acagaggcga ggaaccgtcg cggatgcgcg tcaacgtagt tcggttcgca
     3601 ccgtgtgcgc gcaccgcgtg gcacacacac gcgatgggtc agaccctgca cgtcaccgac
     3661 ggcgtcggcc tcgtgcagtc gcgcggcggt gacgtcgtgg tgatgcaccc cggcgacacc
     3721 gtctacacac cgcccggcga atggcactgg cacggcgctg cgccggagca cttcatgaca
     3781 caccttgcga tgtgggaggg accggaccca gactccggca agaccgaaac ggagtggggc
     3841 gaacacgtga ccgacgacga gtatcaggac cgacgcgagg aatgaacatg gcgaactctt
     3901 cccgggtcac gtctcgactc tggcggcgac cgatcccgaa ctgatcgagg tgttcgacaa
     3961 tttcgcgttc gacgaggttt tacgtgacac caacctcggc tccgcatccg gctgatggtg
     4021 cagccggcat cgatcatcgc cagccaagcc ctcaccgcgc tcaacgaact caccgcggcg
     4081 aaatagacac cgacaaacca attcatacgg cgaaagagtc gccaccgatc actaaggaga
     4141 acttgatcgt gaagcacgcg aagttacgag acctcgacgt ctcgcgaatc ggcttgggcg
     4201 cgatgggcat gtccttcggg tacacgggcg cagggaccga cgacgcggaa tcgatccgca
     4261 cgatccatcg cgccctcgac ctcggcgtca cgttcatcga cacggccgag atctatggcc
     4321 cgtacaccaa cgaggaactg gtcggtcagg cgctcaaggg ccggcgcgac caggtcgtgg
     4381 tggccacgaa gttcggcatg gtgtcgcaca ccggtggcgc gcgaggcaac ttggacagca
     4441 gcccggccaa catccgcacc gccgtagagg ggtccttgcg ccgcctgggc accgactaca
     4501 tcgacctgta ctaccagcac cgcgtcgatc ccaatacgcc gattgaggaa accgtggccg
     4561 cgatggcgga gttggtcacg gagggaaagg tgcgacacct tggcctgtcc gaagccggtg
     4621 tgactaccat tcgccgtgcc cacgcggtgc atccgatcac tgctctccaa tcggagtact
     4681 cgctgtggac gcgtgacccc gaacccgcag tacttcccgt gctgcgcgaa ctgggcatcg
     4741 gcttcgtgcc gtattcgcct cttggacacg gcttcctcac gggcgccgtc cgttcgatgg
     4801 acgatctcga cgaccacgac tggcgcaaga ccagtccccg gttcatgggt gagaacttcg
     4861 accggaacct cgacatcgcc gatgaggtgc aggcaatcgc cgcagaagcg ggcgccacac
     4921 cggctcaggt cgcgatcgcg tggctgctca ccaagggcga cgacatcgca ccgatcccgg
     4981 gcaccaagcg ggtaagccga ctggaggaga acgtcgccgc cgacggcatc gaactcagcg
     5041 ccgagcagct cgagaaactg aacaacctca ccccgcccgc cggcgaccac cacaacgaag
     5101 cgcaaatggc gatgatcgac cgctaactca ccgacagtcc actttcgaag gagaatcatg
     5161 aaagctgcca tcttcaaggg cccaggtgag atcgcgattg gcgaacgacc cgacccggtc
     5221 atcaaggact cgacagatgc agccgtccgc gtcgtgctgg catgcgtctg cggctcggac
     5281 ctgtggtact accgcggcga gacggacttc ccttgagccg tcatcccgac cgccagaaac
     5341 tagcgcagga gttcggtgcc accgacatcg tcgacacccg gggtgaccag gcggtcgaag
     5401 acgtgaagaa cttgaccgaa ggcgtcgggg tggatgccgc cctggaaatg cgtgggcact
     5461 aacgacgcga tgcataccgc gctggcgatc tgccgagccg gtgccatcgt cggcaacgtg
     5521 ggtctgccgc atgacgttga gattccggcg ccggcatctt cgtgcggaat gtcggcgtgc
     5581 acggcggccc cgcttccgtg cgggcctacc tccccgggct gatcgaggac gtgctcgccg
     5641 aacgcatcaa ccccggcaag gtcttcgacc tcaccaccga cctcgatgac atcgccgagg
     5701 cgtacgccgc gatggacgag cggcgcgcaa tcaagtcact ggtgacggtc ggcgatgtcg
     5761 gttcacatga ctaacgcgag ggatcaccgg cggcgggcga agtacttctt gtaggcctcg
     5821 gccgactcgc gcaagccggg atcggcttcg aacttcggct cgaacttcgc ccacgccgcc
     5881 tcgcgcttgg cgggcagata ctccgtcggc caatacgggt gctgctgcgg cgaatagtcg
     5941 cgcagcacgc ggcgggcgac agtggccttg tgcacctcgt cggcgccgtc ggcaagcccc
     6001 atggtcggtg cactcgcgta catcgcctgc agcggcgtga ggtcggtggt gccgagcgag
     6061 ccgaggatgt gcagcgcgtt gaacgacacc tcccgcaaca ccttggccat cgtgaacttg
     6121 acggtggcga tgtccgtgcg ggcgtccttg gtgctggaat tgtcgatttt ccaagcggtt
     6181 tcgagcacca gcaatcgcag catccggatg gcggcgtagg agtcggcgat cttctcctgc
     6241 accatctggt gttcaccgat gagcttgccg tgcgactcac gcgacagcgc ccgctcgcac
     6301 atcatgtcga acgcgagctt gcactgggcg atggtgcgca tcgcgtggtg gatgcggccg
     6361 ccgccgagcc tgcgctgggc gagcgtcttg gcgccgtcct cggggccgag cagatggtcg
     6421 agcggaatac ggacgtcgcg gtagatgatg tggttgtggt tgcggggttc cggtgcgatc
     6481 tccacgcccg gcgtcgtgcg cggcacgacg aacatcccgt tggtgcacat gacgaacagg
     6541 atgtcggcga cgcggccggc ggaggtgaac cacttctcgc cgttgatgac ccactcgtcg
     6601 ccgtcgcgca cggcgtgggt cttgaacag
//
LOCUS       DASYLX010001154         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2616731,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001154 DASYLX010000000
VERSION     DASYLX010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616731"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..901
                     /locus_tag="VGP27_27345"
     CDS             179..901
                     /locus_tag="VGP27_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HEV7855046.1"
                     /translation="MAHSPPLEQTGIGVVTPFDFALDRELWRWMPDDVSLHITRMAYA
                     PMPATLEMAVHISDPALVAKGFDDVRAVSPPVMAYACTSGSFIGGVEGQAALVAAMTE
                     AGAPAAVTTSGALLEALHHLDVAHIATVTPYIAELTVGLTAYLAEAGIEVVAAAGLGL
                     TSDIWTVPYDVTAGLVRDTDNADAQAVFISCTNLPTYDVIATLERELGKPVLTANQVT
                     VWSALRVLGRKAVGPGQQLLES"
     gene            904..>1938
                     /locus_tag="VGP27_27350"
     CDS             904..>1938
                     /locus_tag="VGP27_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV7855047.1"
                     /translation="MPDQAMNDEYNDRLQRVREAMAQKDFAALIVCDPANLFYLTGYN
                     AWSFYTPQCLLVGADGPPHLFTRAMDAAGAGFTANLDHDHIHGYPEELVHRLDVHPFD
                     WIAEQVRGLVPAGETVGVETDSHFFSPRGYQGLERGLPSNRLADSEELVNWVRVVKSA
                     FEIQQLRIAGTIAERAMRAACEGVRPGRRQCDVVAEIMAAQACGTAEHGGDYPAVVPM
                     LPTGKAAGTPHLTWSERTFQVGEATTIELAGVFARYHAPLARTVMLGEPPSRLTDTAK
                     VVAAAMEATLTAIRPGADGSAVHRAFDRVIRPHGLQKDSRIGYSIGIGYPPDWGERTV
                     SLRPGETTELV"
BASE COUNT          336 a          655 c          641 g          306 t
ORIGIN      
        1 gcgcagtagc ggaactccac ctcgaggccg tcgagaccgg gtggacggtc ggtcgccttc
       61 tcgcacagga ccgcgacaac cggtcgtgtt cgaggcacga gtccgaggct aggaacgaaa
      121 ccgtacgatt gtcaacaatc ggcttgcggt cgccacaccg tccccccaga ctggggccat
      181 ggcccattcc ccgccgctgg agcagacagg gatcggagtc gtcacaccgt tcgatttcgc
      241 actcgaccgg gagctctggc ggtggatgcc cgacgacgtc agcctgcaca tcacccgcat
      301 ggcctatgcc ccgatgcctg cgacgctcga aatggctgtg cacatctccg acccggccct
      361 ggtcgccaag gggttcgacg acgttcgcgc ggtctcgccg ccggtgatgg cgtatgcgtg
      421 cacatcgggc agcttcatcg gcggcgtcga gggtcaggca gcattggtgg ccgccatgac
      481 ggaggccggt gcgcccgcag ccgtgaccac cagcggcgcg ctgctggagg cgctgcacca
      541 cctcgacgtc gcccacatcg ccaccgtcac gccgtacatc gccgagctga ccgtcggcct
      601 caccgcctac ctggccgaag ccgggatcga ggtggtcgcc gccgcaggac tgggcttgac
      661 ctcagacatc tggacggtgc cctacgacgt caccgccggg ctcgtgcgcg acaccgacaa
      721 cgctgatgcg caggccgtct tcatcagctg caccaacctg ccgacctatg acgtcatcgc
      781 gacgctggaa cgcgagctgg gcaaaccggt gttgaccgcc aaccaggtga ccgtgtggtc
      841 ggcgctacga gtcctcgggc gcaaggcggt cggccccggc cagcagctgc tggagagctg
      901 acgatgccgg accaggcaat gaacgacgag tacaacgacc ggctgcaacg ggttcgggaa
      961 gccatggcgc agaaggactt tgccgctctg atcgtgtgtg atccggcgaa tctgttctat
     1021 ctcaccggct acaacgcatg gtcgttctac acgccgcagt gcctgctggt cggcgccgac
     1081 ggtccgccgc atctgttcac cagggcgatg gacgccgcag gcgcgggctt caccgccaac
     1141 ctcgaccatg accacatcca cggatacccc gaagagttgg tgcaccggtt ggatgtgcat
     1201 ccgttcgact ggatcgccga acaggtacgc ggtctcgtgc ccgcgggcga aacggtcggc
     1261 gtcgagaccg actcgcactt cttctctcca cgcgggtacc agggtttgga gcgtgggttg
     1321 ccgtcgaatc ggctggccga ctccgaagag ttggtcaact gggttcgagt cgtcaagtct
     1381 gccttcgaga tacagcagtt gcggatcgca ggcacgattg ccgaaagggc gatgcgcgcc
     1441 gcgtgcgagg gggtacgccc aggcagacga caatgcgatg tcgtcgccga gatcatggcg
     1501 gcgcaggcct gcggcacagc cgagcacggc ggtgactacc cggcggtggt tccgatgctg
     1561 cccaccggaa aggccgcggg cacgccccac ctgacctggt ccgagcgcac ctttcaagtc
     1621 ggcgaagcca ccacgatcga gctggccgga gtgttcgccc gctaccacgc gccgctggcg
     1681 cgaacggtga tgctcggtga accgccgtcc cggctcaccg acaccgccaa ggtggtcgca
     1741 gcggcaatgg aagccacgct gacggccatc cggcccggcg cggacggcag cgccgttcat
     1801 cgggcgttcg atcgggtgat cagaccccat gggctgcaga aggattcccg gatcggctac
     1861 tcgatcggca tcgggtatcc gcccgactgg ggtgagcgga ccgtgagcct gcgccctggc
     1921 gagaccaccg aactcgtc
//
LOCUS       DASYLX010001155         9271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1410187,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001155 DASYLX010000000
VERSION     DASYLX010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410187"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGP27_27355"
     CDS             complement(<1..303)
                     /locus_tag="VGP27_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7855048.1"
                     /translation="MTLPPSTAARSVSVGAVFRPQSAPERLAGAARAADAGGLDELWL
                     WEDCFLAGGISAAAIALANSQRLKVGIGVLPVPMRNVALTAMEIATLDRAYPGRIRV"
     gene            828..2141
                     /locus_tag="VGP27_27360"
     CDS             828..2141
                     /locus_tag="VGP27_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV7855049.1"
                     /translation="MTGAMHEELRRGERPLTPDEDPFYEAPGGYEQAPPGAVLRSRVV
                     ELGFLGLIRQHVTATQLLYRTTNLHSEPEVAVTTVLIPGHRDPEKVCPVLSYQCAIDA
                     VSSRCFPSHALRRGAGPIGAFVQAEFLLVAAALAEGWAVSVPDHEGCHGMWGSPYEPG
                     YRILDGVRAALSCERLQLSPSAPVGLWGYSGGGLASAWAAELTDSYAPELNVVGAVLG
                     SPVGDPGSVARRLNRSVFAGLAALMIAALTDVFPGARQVVDQHASVEGRALLEELRTM
                     STAAAVWRLRHVDIGSYADMSADELWDLPEVRHIFEETKLGKSTPRPAVLIVQAVHDP
                     IISVDDIDALVETYSRNGASIAYHRDRFCDHLLLHPLSAPMTLRWLRDRFNGRPLSEH
                     GTRTKWPTLFNPSTYLGLLKLGVISTKVITGRSIDSTPLSKADAS"
     gene            complement(2214..3791)
                     /locus_tag="VGP27_27365"
     CDS             complement(2214..3791)
                     /locus_tag="VGP27_27365"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7855050.1"
                     /translation="MPLASAGTTPPRLAEAAARVSAQTPVDPLRHATFRRWVWGAVLV
                     SVSGGAMVFVLLSFAAPILLSPAATNRLLVRGGTALAVFAIVVVPVLMRVRRNRYATS
                     TAWLRQGRKPTDAERRMTLGAPGEAVRVSALTWGVGAVFFALLGATESISAAAYIFSA
                     VILGGITTTAVWYLIAERIMRPVCARALHGEPAGHRYGPSIQLRLAMAWTLATGVPLL
                     GVATLAVSYLADVGFEPYRTLAVILGLVAVALVVGLFTVVVAARSVAERVTELRRALA
                     RVQAGDFTARVVVDDASEIGRLQVGFNAMAAGLTERERIRDVFGTYVDRDVAEHILQG
                     AALQADEVEVTMMFVDVRSFTTFAERLRPIEVVAALNRLFERIVPLVHRHGGHVDKYA
                     GDGLFAVFGAPRRYVDHADRALKAALEISDAVRDEFGTALSVGVGLNSGPVVAGNVGG
                     AGRLEFSVIGDAVNIAARVESATRETGDSVLLTGQTMALLDADHGEFVARPGLILKGK
                     TAPVHIYAPLVTMCSSS"
     gene            complement(3932..4255)
                     /locus_tag="VGP27_27370"
     CDS             complement(3932..4255)
                     /locus_tag="VGP27_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7855051.1"
                     /translation="MSTTLTLDTARSKDGDFALTAVGEIDMSNIDAFNQALTTATTVT
                     ADSGGTLTVDLSAVEYLDSGAINALVARADHIRIIAHPLLMSALTVSGLNELVTIESA
                     PPEGR"
     gene            complement(4255..6765)
                     /locus_tag="VGP27_27375"
     CDS             complement(4255..6765)
                     /locus_tag="VGP27_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV7855052.1"
                     /translation="MNEQFGELVAARLQMEQLLRVIVEIGSDLDLNVTLHRIVDAAME
                     LTDARYGALGIRASDGSLDSFVHAGIDEDTARGLGNLPVGSEGLRVDDLSSHSQPPAL
                     SAHDPPMRALLAIRITVRGADFGNLYLADDRPGRVFSDSQEGAVRALATAAAAAIDNA
                     RLFERERESARWTKASREITTALLSSDPHTGPLQLIVNRALDLADAEQAILLVPREAD
                     LPADEVDTLVVAATAGRYTSQVIGSQVPMEGSTTGGVARRGLPLITESFQYPIEGFTD
                     VGERSAVVMPLIGDETVLGVIAVARRPGQRPFGNDYLELVSDFARHAALALTLAAGRE
                     HALNQQLAQADTVDDALRAAAEELRRLWRARRVLAVTFPTHRSSARTAEGAPEVVSAG
                     EQAQWLDLPPETRQMLSALRDGDLLTPTTTMPGTVGIAMQHPEGVLVVWIDLAEQRPF
                     TLEDQTLLTVLAGRLGQGLQRVHRVDQQRETALALQHAILGPAHLPGGFAVRYQAASR
                     PLQVGGDWYDVVDLEDGRIGLVVGDCVGHGLAAATVMGQVRSACRALLFENPSPGAAL
                     AGMDRFAARLPGAECTTAVCAVLNPDTGELVYSSAGHPPPVLVHADGSIHLLEGGHTI
                     ALGIRPNWSRPEAHITMPAHATLLLYTDGLVERRRSALDSGISRAAALVRDGRASTLD
                     GLADEVMSRLAPSGGYQDDVVLLLYRHPAPLELKFPAHVSQLAPTRTALRRWLTRARV
                     KPGQAMDVLIAAGEAVANAIEHGHRHSSEGTISVDATAVLDRLQLTITDTGSWKPPQP
                     APDVNRGRGITLMRGLMHDVSIDTGAAGTTVQLSMRII"
     gene            complement(7205..7522)
                     /locus_tag="VGP27_27380"
     CDS             complement(7205..7522)
                     /locus_tag="VGP27_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855053.1"
                     /translation="MTTDNPTAAQRPAAPTAYGAVDAQPTRRPARRGDETKAAFKTTE
                     FIAYVVAVAGVLIASFLVKTTDAHEDYFRADRAWFFVVLLSIGYMVSRGLAKSGSRHH
                     DDA"
     gene            complement(8152..8877)
                     /locus_tag="VGP27_27385"
     CDS             complement(8152..8877)
                     /locus_tag="VGP27_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEV7855054.1"
                     /translation="MTSTYQSRTGRKARIIAGTVLASAASVVGMIGVAAPAQAADQYL
                     ALALGYISENPPVTMAGGSSIAGTQDAAAQGALTNCVNNGGSQCVAVVIATNDCAAAA
                     SNNYGEEVGASAATRGAAETAALAKLQNQTGAKVIVSGCANGSTQPPPPPNDPPPPPA
                     PKLGPTVSFNKVLGGLEAHITDRSGVTSQCTFATDDYNRSFGLPANSTYDLRIVPAVP
                     RFKNWTVTITCDNGTSTTATTYF"
     gene            9050..>9271
                     /locus_tag="VGP27_27390"
     CDS             9050..>9271
                     /locus_tag="VGP27_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV7855055.1"
                     /translation="MPLNGGDVFAGYVIERLLGTGGMGQVYLAQHPRLPRHDALKILA
                     VGSTADREFRARFIREAELAATLSHPHIVR"
BASE COUNT         1654 a         2957 c         3005 g         1655 t
ORIGIN      
        1 gactcggatg cgcccggggt atgcgcggtc aagagtggcg atctccattg cggtcagggc
       61 gacgttgcgc atcggtacgg gcagcacacc gataccgacc ttgagcctct gactgttcgc
      121 cagcgcgatg gcggcagccg aaattccgcc tgccagaaag cagtcctccc acagccacag
      181 ctcgtcgaga cccccggcgt ccgcggcccg cgccgctccg gcgaggcgtt cgggggcgct
      241 ttgtgggcgg aacactgcgc ccacactgac gcttctggcg gccgtcgagg gtggaagggt
      301 catggctggg attctgccgg tatgaggacg tcggcaccaa ggccgaaaga cctggtcacg
      361 gggcggtgtc ggttgcctgt cgattgcggt gccagccgcg gtgcgatctt tggccatgtc
      421 cgcggcaggc gcacactgcg atgacctgca gactgcacac catggggagt aaccgccatg
      481 cggacataca cgcacaacct tagccccgaa gagcgccgtg tcctgctcga gcagcgccgt
      541 gcgatcgtgg cgcgtcaatt acgtcgcctg gcaagggagt tgacagatct cgacagacag
      601 ctccacgaaa ttgtgcagtc agagcgttga ttgggttggc ggccagccgg taggacggat
      661 caaccgacgt cagatggtgt ggcgcgtcag ggttaacaat gcggtaccgc gggtaccgtt
      721 tgcccagtaa agcggtgttt ccgcgcgcaa ataacgcggc cgcttgaaac ctcatcgcta
      781 ggaaagttgg tctgaagatg cagggcgatt ccacagcatg tggattcatg actggagcga
      841 tgcatgagga attgcgtcgg ggcgaacgcc ctctgacgcc cgacgaggac ccgttctacg
      901 aggcgccggg cggctacgag caggcgccgc caggtgctgt gcttcgctcc cgcgtcgtcg
      961 aactgggctt cctcggcctg atccgtcagc acgtcaccgc gacccagttg ctctaccgca
     1021 ccaccaacct gcacagcgag cccgaggtcg ccgtcaccac ggttctcatt cccggacacc
     1081 gtgatcccga aaaggtgtgt cctgtgctct cgtatcaatg cgctatcgac gccgtgtcct
     1141 cgcgatgctt cccctcgcat gcactgcggc gcggcgcggg acctatcggc gctttcgttc
     1201 aagcggagtt tctgctggtc gccgcggcgc tggcagaagg atgggcggtg tcggtgcccg
     1261 accatgaggg ctgtcacggc atgtgggggt cgccgtatga gccgggatac cggatcctcg
     1321 atggcgtacg cgccgcgttg tcatgcgaac gactgcagct gtcgccgtcg gctccggtgg
     1381 gattgtgggg gtactcgggc ggcggactgg cctcggcgtg ggccgccgag ctgaccgata
     1441 gctatgcccc agagctcaac gtcgtcggcg ccgtattggg ctccccggtc ggcgatcccg
     1501 gcagcgtcgc acgccgcctc aatcgcagcg tcttcgccgg ccttgcagcc ctgatgatcg
     1561 ccgcactgac cgacgtcttc cccggcgcga gacaggtggt cgaccagcac gcttctgtag
     1621 agggacgagc cttgctcgaa gagttgcgaa cgatgtccac agccgcggcc gtatggcggc
     1681 tgcgccacgt cgatatcggg tcctacgcgg acatgtccgc cgacgagctg tgggacttgc
     1741 cggaggtacg tcacatcttc gaggaaacca agctcggaaa gtccaccccg cgccccgcag
     1801 tactgatcgt gcaggcggtg cacgacccga tcatcagcgt cgacgacatc gacgcgctgg
     1861 tggaaacgta ctcgcgcaac ggggcctcga ttgcctacca tcgcgaccgg ttctgcgatc
     1921 atttgcttct gcatccgctg tccgcgccga tgacattgag gtggctgcgc gatcgcttca
     1981 atgggcgccc gttgagtgag cacggcacac gtaccaagtg gccaacactg ttcaacccct
     2041 ccacgtacct tggcctactg aagttgggcg tgataagcac gaaagtgatc accggccgtt
     2101 ctatcgacag cactccgctg tcgaaggcgg acgcatcgtg acgcctcact gccgttgcgc
     2161 atccactgcc aaatgcggtc catgcgggga gattaagctt gcgcgacctg cgatcaactg
     2221 gaactgcaca tcgttaccag cggtgcatag atgtgtacgg gtgcggtctt gcccttgagg
     2281 ataagccctg ggcgcgcgac gaattcgccg tgatctgcgt cgagcaaggc catcgtctga
     2341 ccggtcagca gaacggaatc gcccgtctca cgggtagccg actcgacacg cgcggcgatg
     2401 ttgacggcgt cgccgatgac ggaaaactcc agccgtccgg caccgccgac atttccggcc
     2461 accactggtc cggagttcaa cccgacgccc actgacagcg ccgtcccgaa ctcgtcgcgc
     2521 accgcgtcgg agatttccag tgcggctttc aacgcccggt cagcgtgatc cacgtagcgc
     2581 cgtggcgctc cgaagaccgc gaaaagcccg tcgcccgcgt atttgtcgac gtgtccgccg
     2641 tgtcggtgca ccagcggaac gatccgttcg aagagacggt tgagtgccgc gacgacctcg
     2701 atgggccgaa gccgttctgc gaaggtggtg aagctgcgga cgtcgacgaa catcatcgtg
     2761 acttccacct cgtcggcctg cagagcggcg ccctgcagga tgtgctcggc aacatcgcgg
     2821 tcgacgtacg tcccgaacac atcccgaatg cgttcccgct cagtcagacc cgccgccatg
     2881 gcgttgaagc cgacctgtag gcgcccgatc tcgctggcgt cgtcgacgac gacgcgtgcc
     2941 gtgaagtcgc cggcctggac gcgggccagt gcgcggcgaa gttctgttac ccgctcggcg
     3001 acagaccgcg cagcgacaac gacagtgaac aacccgacca cgagtgctac ggcgaccagg
     3061 cccaggatca cggccaacgt tcgatacggc tcgaatccga cgtccgcgag gtaactcacc
     3121 gccagcgttg cgacgccgag cagcggcact cccgtcgcca gcgtccacgc catcgccagt
     3181 ctgagctgga tactcggccc gtaccggtga ccggccggct caccgtgtag cgcgcgcgca
     3241 cacaccggcc gcatgatccg ctcggcgatc agataccaga cggcagtcgt ggtgatgccg
     3301 cccaagatca ctgcgctgaa gatgtatgcc gcagccgaaa tcgattccgt cgcacccaac
     3361 agcgcgaaga acacggcacc gacgccccat gtcagggcag aaacgcgaac tgcttcgccc
     3421 ggcgccccga gcgtcatccg tcgttccgcg tccgtcggtt tgcggccctg ccgcagccag
     3481 gccgtgctcg tcgcatatcg attcctgcgg acacgcatca ggaccggcac cacaacgatc
     3541 gcgaacacgg ccaacgccgt tccgcccctg accaggagcc gattcgtcgc ggcaggactc
     3601 aacagaatcg gcgctgcgaa ggacaggaga acgaaaacca tcgcgccacc ggacacactg
     3661 accagcacgg caccccacac ccagcgcctg aacgtcgcat gccgcagtgg gtcaaccggt
     3721 gtctgggcag aaacgcgcgc ggccgcctcg gccaatcggg gcggcgtcgt accggcggag
     3781 gctaagggca tgtacctgag cgtggtcgcc gactgtggga ccggctagcg tagtccgcta
     3841 ctcgactgaa cagacacagt gtggggatat gtgcttagct gccccctcag caaaggtaaa
     3901 cggcgccatt aagaaagctg ttggagtccg gtcatcgtcc ttcgggcggc gcggattcga
     3961 tggtgaccag ttcattcagg ccgctaacgg tgagcgcgga catcagcagg gggtgcgcga
     4021 tgattctgat gtggtcggcg cgggcgacca aggcgttgat cgcgccgctg tccaggtact
     4081 cgacggcgct gaggtcgacc gtcagcgttc cgccgctgtc agccgtcacg gtggtcgcgg
     4141 tggtgagggc ttggttgaag gcgtcgatgt tgctcatatc gatctccccc accgctgtca
     4201 gcgcgaaatc gccgtccttg ctacgcgctg tgtcgagggt gagcgtcgtg gacatcagat
     4261 gatcctcata gatagctgaa ctgtggttcc ggcggcgccg gtgtcgatgc tgacgtcgtg
     4321 catgagccct cgcatcagag tgataccccg gccacgattg acgtcgggag ccggttgcgg
     4381 aggtttccac gagccggtgt cggtgattgt caactgtaac cggtcgagca ccgcggtcgc
     4441 gtccacgctg attgttccct ccgaactgtg gcggtggccg tgctcgatcg cgttggcgac
     4501 ggcttctccg gcggcgatga gcacgtccat ggcctgaccc ggcttcaccc gagctcgggt
     4561 cagccagcgg cgtagggccg tgcgagtggg ggcgagttgg ctgacgtgag cggggaactt
     4621 cagctccagc ggggcaggat ggcgataaag cagaaggacg acgtcgtcct gatagccgcc
     4681 gcttggtgca agacgggaca tgacctcgtc cgccaaccca tccagtgtgg aagcccggcc
     4741 gtctcgaacc agtgcggcgg cgcgggagat gccgctgtcc agtgcactgc ggcgacgttc
     4801 gaccagcccg tcggtgtaga gcagcagggt cgcgtgcgcc ggcatcgtta tgtgggcttc
     4861 ggggcgtgac caattggggc gtattcccaa cgcaattgtg tggccacctt cgagcaaatg
     4921 aatgctgccg tcggcgtgga cgaggacagg cggcgggtgc ccggcactgg agtacaccaa
     4981 ttcgcctgtg tcggggttga gcaccgcaca caccgcggtg gtgcattcgg cgccggggag
     5041 tcgggcagca aaacgatcca taccagcgag agcggcgccc ggacttgggt tctcgaagag
     5101 cagcgcgcgg caggcgctgc gaacctgtcc catcacagtg gcggcggcca agccatggcc
     5161 gacacagtca ccgacgacca acccgatgcg cccgtcctcc aggtcgacga cgtcgtacca
     5221 gtcaccaccc acctgaaggg gccggctggc agcctgataa cgcacagcga acccgcccgg
     5281 tagatgagca gggccgagga tcgcatgctg cagtgccagg gcggtttcgc gctgctgatc
     5341 aacccggtgc acccgctgca ggccttggcc aaggcgccct gccagcaccg tcagcagagt
     5401 ttggtcctcc agggtgaatg gccgttgctc tgccagatcg atccagacga cgagcacgcc
     5461 ctcgggatgt tgcatcgcga tgcccaccgt cccaggcatt gttgtggtgg gcgtgagtag
     5521 gtcgccgtcg cgcaaggcgc tgagcatctg tcgtgtttcg ggtggcaggt cgagccactg
     5581 cgcctgctca ccggcagaca ctacttccgg tgcgccttcg gccgttctcg ccgaggatct
     5641 gtgggtgggg aaggtgacgg ccagaacgcg ccgtgcccgc cataaccggc gcaattcctc
     5701 agcggcggcg cgtagtgcgt cgtcgacggt atccgcttgt gcgagttgct ggttgagcgc
     5761 atgttcgcgg cctgccgcca acgtcaacgc aagagcagcg tgccgggcga agtcactgac
     5821 gagctcgagg taatcgttcc cgaatggcct ctgccccggg cggcgtgcga cagcaatcac
     5881 accaagtacc gtctcgtccc caatcagcgg catgacgacc gccgagcgct caccgacatc
     5941 cgtgaagccc tcgatcggat attggaaaga ttccgtgatc agaggtagac cgcgacgcgc
     6001 aacaccgccc gtggtggagc cttccatcgg aacctgacta ccgatcactt gcgaggtgta
     6061 ccggcccgcc gtagcggcca ccacaagggt gtcgacctca tcggcgggca gatcggcctc
     6121 cctcggaaca agcaatatcg cctgctctgc atcggccaag tccaacgccc ggttcacgat
     6181 gagctgcaac ggcccagtgt gggggtcgct ggacagcaga gcggtcgtta tctcgcgact
     6241 ggccttcgtc catctcgccg attcgcgctc ccgctcgaac agtcgcgcgt tgtcgatggc
     6301 ggccgctgcc gcggtcgcca atgcccgcac agcgccttcc tgggagtcgg agaacactcg
     6361 accaggccgg tcgtcggcca ggtaaaggtt tccgaagtcg gctccccgca cggtgatccg
     6421 tattgcgagg agcgcccgca tcggcggatc gtgtgcgctc agcgcgggag gctgcgaatg
     6481 agaactcagg tcgtcgacgc gaagaccttc actacctacg ggcagattgc caagcccccg
     6541 cgccgtgtcc tcgtcgatcc ccgcatggac gaatgagtcc aggctgccgt cggacgcgcg
     6601 tatacccagc gctccgtacc gggcgtcggt cagctccatc gccgcatcga cgatccgatg
     6661 cagcgttaca ttcaagtcca gatcggaacc gatctcgacg atcacccgca gcagttgctc
     6721 catctggagg cgagcggcca ccaactcgcc gaactgctca ttcatacgcc acaccggctc
     6781 ccgccgacgc cgccagacga acgccgaccc cgcccgtgag aatcgacgat gcagtagcgc
     6841 catctagcgt agtaagccac cacgccacgc gaacccggat gatcgtgctt caaccctgca
     6901 acatttctat ccttcacgct ggggtctacc ccgtgcacct cgggggtaaa actagttgtt
     6961 gggtccaaca tggtcgaccg ttcgcgcatc tctcgccggt tttacccggc gagccggcgg
     7021 acccacccag taccgggcag cgagtttgag cggcttggcg gctttggagt cgcgagaagc
     7081 gtgcatgaga gacgcccccg ccattgcaag acagcagtgg cgggggcaat caatcggtga
     7141 gggctaacgt cctcgactct ccagtcgccg cgttgttctg agcaactgcg ggtcaataga
     7201 ggttttaggc gtcgtcgtgg tggcggctac cggacttcgc cagaccgcgg ctcaccatgt
     7261 agccgatgct tagaagaacc acgaagaacc atgcccggtc ggcccggaag tagtcctcgt
     7321 gcgcgtcagt ggttttgacg aggaacgaag cgatcagcac gccggcgacc gccacgacat
     7381 acgcgatgaa ttccgtagtc ttgaaagccg ctttggtttc gtcaccccga cgggctgggc
     7441 gacgagtcgg ttgagcgtcg acagcgccgt aggcggtggg agcggctggc cgctgtgcgg
     7501 cggtgggatt gtcagtggtc attaagatat ctccttctga ggccggccga cgtgttatcg
     7561 aaccgacagt gatggtgtgc aaccttcgac gggggtaaac gcgaattgct ctgacaacag
     7621 accgaattcg ggcgtcgagc aaccagcgcg tttcgtcaag cgactcggat gcgccgcttg
     7681 acggtacgac gagtcgccca ccgctaacga ctggttgatt gaggtccgtc ttgggctgag
     7741 ggtcgcggca tacgatgaac gagaccgctg cgaactgagt tgataccgat gtctaaagca
     7801 tcaactgtgt gaagagtacc cgcgagtaac actttctaat cgtctgcgct acgcatctca
     7861 agcgccctca gcccaagtcc tggataagcg cgaatgtggt cgaccggtcg cgcccatcaa
     7921 cacgggccca cggcgcgcat tgtcagcgcc ttcatgctca ttccgcaccg tatggcactg
     7981 accgcaacca acgtcggctc acgattgtca aagcccttgt gacggtggcg aagggaaccc
     8041 tgttgattca acgcaacatt tgttaactaa ccgctgatgg cattgataac gcgaaaccgc
     8101 ccgggccgtg gtttcacggt ccgggcggtt cggtgtggag gaacgcgcgg ctcagaagta
     8161 cgtcgtcgcc gtcgtgctcg ttccgttgtc gcacgtgatc gtgacggtcc agttcttgaa
     8221 gcgcggaacg gccgggacga tcctcaggtc gtacgtggag ttggccggca acccgaagct
     8281 gcggttgtag tcgtcggtcg caaacgtgca ctgcgacgtc actccgctgc ggtcggtgat
     8341 gtgtgcctcc agtccgccca agaccttgtt gaacgagacc gtgggtccca gcttcggcgc
     8401 ggggggaggc ggcgggtcat tcggcggcgg cgggggctgg gttgacccgt tggcgcagcc
     8461 ggacacgatg accttggcgc ccgtctggtt ctgcagcttg gccagggcag cggtctccgc
     8521 tgcgccgcgc gtggcggcgg aggctccgac ctcttcgccg tagttgttcg aggcggccgc
     8581 cgcgcagtca ttcgtggcga tgaccacggc gacgcactgg ctgccgccgt tgttcacaca
     8641 gttggtcagc gcgccctggg cggccgcgtc ctgcgtgccg gcgatcgacg agccgccggc
     8701 catggtgacc ggggggttct cgctgatata tccgagagcc agtgcgaggt actggtctgc
     8761 tgcctgcgca ggcgcggcga cgccgatcat gccgacaacg gacgctgccg aggccagcac
     8821 ggtgccggcg atgatccggg ccttccggcc ggtacggctc tggtaggtgc tcgtcatgat
     8881 gtctcctgtt tggtggtcgc tgccccgcgg gctgcgttca agagaaatac ttgcgttgct
     8941 cgaggggcgg gagaatctcc gcgaagcgag aatccaccga cggaaaggga gactttccta
     9001 tccgcgaggt gagcatcgac ggcacgcgct cccccgtagg ctttcggtca tgccgttgaa
     9061 cggcggtgac gtcttcgccg ggtatgtgat cgagaggttg ctcggcaccg gtggcatggg
     9121 ccaggtttac ctcgcccagc atcctcgtct acctcgtcat gatgcgctca agatcttggc
     9181 cgtgggctca accgccgaca gggagtttcg ggcgagattc attcgagaag ccgaattggc
     9241 cgcgacgctc tcacacccac atatcgtccg c
//
LOCUS       DASYLX010001156         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3497300,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001156 DASYLX010000000
VERSION     DASYLX010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3497300"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGP27_27395"
     CDS             complement(<1..120)
                     /locus_tag="VGP27_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7855056.1"
                     /translation="MSETPNGMSRREELLAVATRLFAARGYHGTRMDDVADAVG"
     gene            268..378
                     /locus_tag="VGP27_27400"
                     /pseudo
     CDS             268..378
                     /locus_tag="VGP27_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            complement(405..554)
                     /locus_tag="VGP27_27405"
     CDS             complement(405..554)
                     /locus_tag="VGP27_27405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855057.1"
                     /translation="MQSTTDEVLRGQPPNLNEPTAKGLPTDRELELLRQQRIHFDGFF
                     TGPTW"
     gene            complement(684..1163)
                     /locus_tag="VGP27_27410"
     CDS             complement(684..1163)
                     /locus_tag="VGP27_27410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855058.1"
                     /translation="MDSSAVVWIVVAVVAVIVIAALVFAARNRRNQRRHVEAERIRDD
                     LDTHVAKVDKREALADETAARARAAQAEAEAKAAEAARLNERAESHRGVVAESREDIE
                     KRRQHADRLDPRAGVQTDGAQHDAVRRDDVDYDAAAAQRAEQDATAQRAAGREPERR"
     gene            1432..>1787
                     /locus_tag="VGP27_27415"
     CDS             1432..>1787
                     /locus_tag="VGP27_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEV7855059.1"
                     /translation="MIIGIPRESLPGETRVAATPQTVGQLTKLGYEVVVETGAGAASS
                     FSDAAFSEAGAGVGSGEQVWAADVVLKVNAPDTAEIAALRDGATLVGLISPALRPELV
                     EQLATRPITVLAMDAVP"
BASE COUNT          273 a          610 c          584 g          320 t
ORIGIN      
        1 cccgaccgcg tcggccacgt cgtccatccg ggtgccgtgg tagccccgcg cggcgaacaa
       61 cctggtcgcg accgccaaca gctcctcgcg gcgggacatc ccgttcggcg tctcggacat
      121 ggcgactcac tataggcagg ccggggccca cataccccgg ggcgatcaat caactggttg
      181 gccagtctag atgcaccggg tgccggcacc gtcgccgcgg gtctagactt ccgcctagac
      241 acagccgata cgcgagaagg tgagcacatg gatccgaatc cggattatga cgccagcgac
      301 gagctggagt acggcgtcaa ctggttcgcc tggatcctgc gaggcgcgta tccaccgccc
      361 gcctatcccc cggtttaagc aactcctcga taagagcgcg ccggctacca cgtggggccg
      421 gtgaagaacc cgtcaaagtg gatgcgctgc tgacgcagca attccagttc tctgtctgtc
      481 ggcagcccct tggccgtcgg ctcgttcaag ttcggcggct ggccccgcag cacctcatcc
      541 gttgtcgatt gcatgaattc atcgtttccg gccagcgccg tgatggcgcg agtagtgcac
      601 tactgcagtt tgtttccaca tgaatgcatg gcagtgccgc gctctaggaa tacgccgatc
      661 ccgaacgcgg cactgggctt gggttacctg cgctcgggtt cccgaccggc ggcccgctgg
      721 gctgtggcat cctgctcggc gcgctgcgct gcagcggcgt cgtaatcgac atcgtcccgc
      781 ctgacggcgt catgctgggc gccatcggtc tgcaccccgg cccggggatc cagtcgatcg
      841 gcgtgctggc ggcgcttctc gatgtcctcc cgcgactcgg cgacaacgcc tcggtggctt
      901 tccgcccgct cgttgagtcg ggcggcctcc gccgccttgg cctcggcctc cgcttgcgcc
      961 gcgcgggcgc gcgccgcggt ttcgtcggcc agggcctcgc gcttgtccac cttggcgacg
     1021 tgcgtgtcga ggtcgtcgcg gatgcgctcg gcttcgacgt gcctacgctg gttgcgcctg
     1081 ttgcgcgccg cgaacaccag cgcagcgatc acgatgacgg cgactacggc gacaacgatc
     1141 cagacgacgg cgctgctatc catggcgaat cctttcgtcg gaaaaatcct gcggcgctga
     1201 cgcgcccgct aattaaggat tcccctcgga acagccgccg aaacttacgg gcggaagcgc
     1261 tcgatgaccg tggacctgct tactcgggta caccggcacg tgcctacagg gcgcgccgtc
     1321 gatttgagcc gactcttaca ctgacgtatt cactcgtgca acgacgagct gtgtcggggc
     1381 gctttcgcac tccgaccgga ccgtgacaga acctagatcg gatcgaaatc gatgatcatc
     1441 gggatcccga gagagtcgct gcctggagag acgcgcgtcg ccgcgacgcc gcagacggtc
     1501 gggcaactca ccaagctggg ctatgaggta gtggtcgaga ccggcgcggg cgccgcctcg
     1561 agcttctccg atgcggcgtt ttcggaggcc ggtgccggtg tcgggtccgg cgagcaggtc
     1621 tgggcggccg atgtggtgct gaaggtcaac gcgccggaca ccgcggagat cgccgccctg
     1681 cgcgacggcg ccacgctggt cggcctgatc tcccccgcgc tgcggccgga gctcgttgag
     1741 cagctggcca cccggccgat caccgtgctg gccatggacg ccgttcc
//
LOCUS       DASYLX010001157         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1850003,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001157 DASYLX010000000
VERSION     DASYLX010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850003"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGP27_27420"
     CDS             <1..182
                     /locus_tag="VGP27_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7855060.1"
                     /translation="FGGTTPYLVAWLTGSTGNNLAPAFYVIVAAVVSLIAVLTVRESA
                     GRPLPAVATAEVAAL"
     gene            179..871
                     /locus_tag="VGP27_27425"
     CDS             179..871
                     /locus_tag="VGP27_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV7855061.1"
                     /translation="MTHREFTAYGPSHWAVLAVFVIVAALLVWIGRKQTDAQARLLGR
                     FLGALTATIYAAVLIYSLIPPSIDSSVPLQLTDLATTAGAYALWSQRHWAVALTYYWG
                     LVLSVQALISPVLRGPDFPNYQFIAFWAIHLLVVWAAIYLTWGRGFRPTWRDYRLVIG
                     GTLAWAAITLTFNRIAGTNYGFLNRKPSTPSLLDLFGPWPVYVLVAAILISIVWALMT
                     WPWVRKARRLPS"
     gene            990..1649
                     /locus_tag="VGP27_27430"
     CDS             990..1649
                     /locus_tag="VGP27_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7855062.1"
                     /translation="MPVALSFQDTIALLHLGDDENRFSPEFLDQVEAQIDDAISLGAH
                     GLVTVATGKFYSNGLDLDWLAAHGDQTQWYVGRVQALLARMLTLPIPTAAAIGGHSFG
                     AAAMLAMAHDFRVMRADRGYFCFPEVDIRIPFTPGMAALIQAKLTPQTAVAAMTTGRR
                     FGGAEAAAAGIVDATAEEDAVVGAALNMLAPLGAKDPGTLGAIKNTMFATVVAALRAV
                     S"
     gene            complement(1671..>2868)
                     /locus_tag="VGP27_27435"
     CDS             complement(1671..>2868)
                     /locus_tag="VGP27_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEV7855063.1"
                     /translation="VVKTTSDPYVGRVSLVRVFSGTIRPDATVHVSGHFASFFGSSGS
                     NGSTGAHADHDEDERIGTLSFPLGKQQRPAGTVVAGDICAIGRLSKAETGDTLSDKAD
                     PLVLKPWTMPEPLLPVAVQPRAKTDDDKLSVGLQRLAAEDPTLRIDQNPETHQIVLWC
                     MGEAHSGVLLDGLANRFGVSVDKVDLRVPLRETFGGPAKGHGRHVKQSGGHGQFAVCD
                     IEVEPLPEGSGFEFVDKVVGGSVPRQFIPSVEKGVRAQMEKGVGPGYPVVDIRVTLLD
                     GKAHSVDSSDYAFQMAGGLALREAANAARVNLLEPVDEVSVMVPDDLVGAVMGDLSSR
                     RARVLGTENVGEDRTVVKADVPHVELTRYAIDLRSLSHGAGSFTRSFARYEPMPESAA
                     ARVRSG"
BASE COUNT          456 a         1006 c          910 g          496 t
ORIGIN      
        1 cgttcggcgg cacgacgccc tacctggtcg cctggctgac cggaagcaca ggtaacaacc
       61 tggcgcccgc gttctacgtc attgtcgcgg cggtggtttc gctcatcgcc gtgctgacgg
      121 tgcgggagtc cgcgggccgc ccgctgcccg cggttgccac cgcggaggtc gcggccctct
      181 gacacaccgg gagttcacgg cgtacggccc gtcgcactgg gccgtactcg ccgtgttcgt
      241 gatcgttgcg gcgctactcg tctggatcgg ccgcaagcag accgacgcgc aggcccgctt
      301 gctcggcaga ttcctcggcg cgctgaccgc aaccatctac gcggcggtcc tgatctactc
      361 gctcatcccg ccgtcgatcg actcgtcggt gccgctgcag ttgaccgacc tcgccactac
      421 cgcaggcgca tacgcactgt ggtcgcaacg acattgggca gtcgcactga cctactactg
      481 gggactggta ctcagcgttc aggcgctcat ctctcctgtg ctgaggggcc ccgacttccc
      541 caactatcag ttcatcgcct tctgggcgat acacctgctc gtcgtctggg cggcgatcta
      601 cctgacctgg ggccgtgggt tccgcccgac gtggcgcgac taccggctcg tcatcggcgg
      661 cacactggcg tgggccgcga tcacgctgac gttcaaccgc attgccggca cgaactacgg
      721 tttcctgaac aggaagccga gcacgccgtc tctgctcgac ctgttcggtc cgtggccggt
      781 atacgtgttg gtcgctgcca tcctcatctc gatcgtctgg gcgttgatga catggccgtg
      841 ggtgcgcaaa gccaggcggc taccctcgtg aggtggcttg cgcacagcgt cgtaccccca
      901 tcgtcaccga catcgaagac gaccgacgtc acgcgcagcc gttgcttctg acccgtcacc
      961 tgctcgtcct accggagtga atcgagccaa tgcccgtcgc cctcagcttt caggacacga
     1021 tcgcgctgct gcacctcggc gacgatgaaa accgcttctc cccagagttt ctcgatcagg
     1081 tcgaggccca aatcgacgat gcgatatcgc ttggagcgca cgggttggtc accgtggcga
     1141 ccgggaagtt ctactcgaac ggcctcgacc tcgactggct cgcggcacac ggcgatcaga
     1201 cgcagtggta tgtcggccgc gtgcaggcgc tgctggccag gatgctgacg ctaccgatac
     1261 ccaccgccgc cgcgatcggc ggacacagct tcggcgccgc cgcaatgctg gccatggcgc
     1321 acgactttcg cgtgatgcgc gccgaccgtg gatatttctg cttccccgaa gtagacatcc
     1381 ggattccgtt cacaccgggc atggccgcgt tgattcaggc caagctgacc ccgcagaccg
     1441 ccgttgccgc catgaccacc gggcgccggt tcggcggggc cgaggccgcc gcggccggaa
     1501 tcgtcgatgc gaccgcggaa gaggacgcgg tggtcggcgc ggccctcaac atgcttgcgc
     1561 cattgggcgc caaggatccc ggcacgctgg gtgcgatcaa gaacaccatg tttgcgaccg
     1621 tggtcgcggc actgagggcg gtttcctagc gtcgcgcacg cgaggagcaa ttagcccgag
     1681 cgcacgcgcg ctgcggcaga ctccggcatg ggttcatagc gggcgaagga gcgggtgaac
     1741 gacccagcgc cgtgggacag cgagcgcaga tcgatcgcgt agcgcgtcaa ctccacgtgc
     1801 ggcacgtcgg ctttcacgac cgtacggtcc tcgccgacgt tctcggtgcc cagcacgcgg
     1861 gcccggcggc tggacaggtc gcccatcacc gcaccgacga gatcgtcggg caccatcacc
     1921 gagacctcgt cgaccggctc cagcaggttc accctcgcgg cgttcgccgc ctcgcgcagc
     1981 gccagcccgc cggccatctg gaacgcgtag tccgacgagt ccacgctgtg cgccttgccg
     2041 tcgagaagcg tgacgcggat atcgaccacc gggtagccgg gcccgacgcc cttctccatc
     2101 tgcgcgcgta cacccttttc cacgctcggg atgaactgcc gcggcacgga gccaccgacc
     2161 accttgtcga cgaactcgaa cccactgccc tctggcagcg gctccacctc gatgtcgcag
     2221 acggcgaact ggccgtgtcc gccggactgt ttgacgtgcc tgccgtgtcc cttggccggt
     2281 ccgccgaacg tttcccgcag cggcacacgc agatccacct tgtcgacact gacgccgaac
     2341 cggttcgcca acccgtcaag caggacgccg gagtgcgcct cgcccatgca ccacagaacg
     2401 atttggtggg tctccgggtt ctggtcgatg cgcagcgtcg ggtcttcggc cgccaaccgc
     2461 tgcaagccga ccgacagctt gtcgtcgtcg gtcttggcgc gcggctgcac ggcgacgggc
     2521 aggagcggct cgggcatcgt ccacggtttc agcacgagcg gatcggcctt gtcggacaac
     2581 gtgtctccgg tctcggcttt cgacagcctg ccgatcgcgc agatgtcacc cgccaccaca
     2641 gtgccggcag gccgctgctg cttgccgagc gggaaggaca gtgtgccgat ccgctcgtcc
     2701 tcgtcgtgat ccgcgtgagc accggtggag ccgttggatc cggaggagcc gaaaaaggaa
     2761 gcaaaatggc ccgacacgtg aaccgtcgcg tcgggcctga tggtgccgga gaagacccgg
     2821 accaggctca ccctgccgac gtagggatcc gacgtcgtct tgaccacc
//
LOCUS       DASYLX010001158         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1740590,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001158 DASYLX010000000
VERSION     DASYLX010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740590"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGP27_27440"
     CDS             <1..591
                     /locus_tag="VGP27_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV7855064.1"
                     /translation="GILTYELLTGTTPFTGDSALAVAYQRMDHDVPRPSAVISGVPRQ
                     FDELVLRATARDPAGRYTDAGDMAAELEAIVDELRLPDFRVPAPRNSAQHLSAELHRS
                     RATHNATTDTVRPAPPRQHTRELTRGPDDWQSDEPETEHQGLPGRFAGIDMDEFHWAR
                     QRARRVLFFWVIAVLVLTGSLAAAAWTIGSNLSGLI"
     gene            complement(616..2004)
                     /locus_tag="VGP27_27445"
     CDS             complement(616..2004)
                     /locus_tag="VGP27_27445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEV7855065.1"
                     /translation="MNWTVDVPIEQLPDLPPLPDDLRHRLDAALAKPAAQQPSWDAGQ
                     AAAMRTVLESVPPVTVPSEIERLKGLLADVALGKAFLLQGGDCAETFVDNTEPHIRAN
                     IRTLLQMAVVLTYGASMPVVKVARIAGQYAKPRSADIDSLGLKSYRGDMINGFAADAA
                     VREHDPSRLVRAYANASAAMNLVRALTSSGLASLHQVHDWNREFVRTSPAGARYEALA
                     GEIDRGLTFMSACGVDDRNLQTAEIYASHEALVLDYERAMLRLSRDEEGTPKLYDLSA
                     HYVWIGERTRQLDGAHVAFAEVIANPIGVKIGPTMTPELAVEYVERLDPHNQPGRLTL
                     VSRLGNNKVRDLLPPIIEKVQATGHQVIWQCDPMHGNTHESSTGYKTRHFDRIVDEVQ
                     GFFEVHRALGTHPGGMHVEITGENVTECLGGAQDISDSDLAGRYETACDPRLNTQQSL
                     ELAFLVAEMLRD"
     gene            complement(2111..2590)
                     /locus_tag="VGP27_27450"
     CDS             complement(2111..2590)
                     /locus_tag="VGP27_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyadenylate-specific 3'-exoribonuclease AS"
                     /protein_id="HEV7855066.1"
                     /translation="MRYFYDTEFIDDGRTIELISIGVAAEDGREYYAISSEFDPERAG
                     RWVRTHVLPKLPPPSSQLWRSRRQIRSELEDFFGTDGDEPIELWAWVGAYDHVVLCQL
                     WGPMTALPPAIPRFTRELRQFWEERGCPRMPPRPRDAHDALVDARHNLRRFQLMTGL"
     gene            complement(2599..3483)
                     /locus_tag="VGP27_27455"
     CDS             complement(2599..3483)
                     /locus_tag="VGP27_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855067.1"
                     /translation="MHLSFDLGIGIPHGRLPLVFCLVAFILTFFVTRTIVRYIRSHAD
                     SDKPRRWWQPRNISAGGGVHIHHVVIGVILVMISGIAMVTLAVNGGVPEFTVAATFFG
                     IGAALVLDEFALILHLQDVYWAEDGRTSVDAVFAAIAVAGLLILGFNPLSFFDVGIWR
                     SDHSVAARAVVIALAVATLALAVIVLLKGKVWTGLVGMFITPLLFIGAIRLSRPHAPW
                     ARWRYTNKPRKMHRALLRERTLRRPVVQAKLWLQHVVAGEPHFPDDREVNEQLDREIH
                     PAPAPPTDTPPVSEKATV"
BASE COUNT          587 a         1184 c         1161 g          562 t
ORIGIN      
        1 ggcattctca cctacgagct gctgaccggc accaccccgt tcaccggcga ctccgcgctg
       61 gcggtggcat accagcggat ggaccacgac gttccccgac ccagcgccgt gatcagcggt
      121 gtgccaagac aattcgacga attggtgcta cgtgcgaccg cgcgcgaccc ggccggccgt
      181 tataccgatg caggagacat ggcggccgaa ctcgaggcga tcgtcgacga attgcgcctg
      241 cccgacttcc gggtgcccgc gccgcgcaac tccgcgcagc atctgtcggc ggagttgcac
      301 cgcagccggg cgacccataa cgccaccacc gacaccgtcc gcccggcgcc gccgcgtcag
      361 cacacccggg agctcacccg cggtcccgac gactggcagt ccgacgaacc ggaaaccgaa
      421 catcaaggcc tcccaggacg attcgccggg atcgatatgg acgagttcca ctgggcgcgc
      481 cagcgcgcca ggcgcgtgct gttcttctgg gtgatcgccg tgctggtcct caccggttcg
      541 ctggccgccg ccgcgtggac gatcggcagc aacctgtccg ggctgatctg aggcgcgcaa
      601 catctccgcc cctgcctaat cgcggagcat ctccgcgacc aggaacgcca actccagcga
      661 ctgctgggta ttgagccgcg gatcgcatgc ggtctcgtag cggcccgcca gatcggagtc
      721 cgagatgtcc tgtgcgccgc cgaggcattc ggtgacgttc tcgccggtga tctcgacgtg
      781 catgccgccg ggatgcgtgc cgagcgcccg gtgcacctcg aagaagccct gcacctcgtc
      841 gacgatgcgg tcgaagtggc gggtcttgta gccggtggac gactcgtggg tgttgccgtg
      901 catcgggtcg cactgccaga tgacctggtg acctgtcgcc tgcacctttt cgatgatcgg
      961 cggcagcagg tcgcgcacct tgttgttgcc gagccggctg accagcgtca ggcgaccggg
     1021 ctggttgtgc gggtcgaggc gctcgacgta ctcgacggcc agctcgggcg tcatcgtcgg
     1081 gccgatcttc acgccgatgg ggttggcgat cacttcggcg aacgcgacgt gcgccccgtc
     1141 gagctggcgg gtgcgctctc cgatccacac gtagtgcgcc gacaggtcgt aaagcttggg
     1201 cgtcccctct tcgtcgcgtg acagccgcag catggcccgc tcgtagtcga gcaccaaagc
     1261 ctcgtggctg gcgtagatct cggccgtctg caggttgcgg tcgtcgaccc cgcacgcact
     1321 catgaacgtc agcccgcggt cgatttcacc ggcgagcgcc tcgtagcggg cgcccgcggg
     1381 cgaagtccgg acgaactccc ggttccagtc atgcacctgg tgcagcgagg cgaggcccga
     1441 cgacgtcagg gcccgcacca ggttcatcgc ggcgctggcg ttggcgtagg cacggacaag
     1501 ccgggacgga tcgtgctcgc gcaccgccgc gtccgccgcg aaaccgttga tcatgtcccc
     1561 gcggtaggac ttgaggccga gggagtcgat gtcggccgag cgcggtttgg cgtactgacc
     1621 ggcgatgcgg gcgaccttga ccacgggcat gctggcgccg taggtgagta ccaccgccat
     1681 ctgcagcagc gtgcggatgt tggcgcggat gtgcggttcg gtgttgtcga cgaacgtttc
     1741 cgcgcagtcc ccgccctgca acaggaaggc cttgccgagg gcgacatcgg ccagcaggcc
     1801 cttgagcctc tcgatctcgg agggcaccgt caccgggggc acgctctcca gcacggtgcg
     1861 catcgccgcc gcctgcccgg cgtcccagct cggctgctgc gcggccggct tggccagtgc
     1921 ggcgtccagc cgatgtcgca ggtcatccgg cagcggcggc aggtccggca gctgctcgat
     1981 gggtacgtcg acagtccagt tcacccatac atggtaacca gcgcaaaccg gtattccaca
     2041 ccgcgggccg gagcaggcga cgggcgtttt atcgtttgca ttcaaccaat ggggtggcag
     2101 ttccccgcca tcagagaccg gtcatcagct gaaaccggcg caagttgtgc ctggcgtcga
     2161 cgagggcgtc gtgcgcgtcg cgcggccgcg gcggcatccg cgggcatccg cgttcctccc
     2221 agaactggcg aagctcacgg gtgaaccgcg ggatggcagg cggcagtgcg gtcatcggcc
     2281 cccacagctg acacagcacc acgtggtcgt aggcgccgac ccaggcccac agctcgatcg
     2341 gctcgtcgcc gtcggtgccg aagaagtcct ccagctcaga tcgaatctgt ctgcgcgacc
     2401 gccacagttg cgacgacggc ggcggcagct tgggcaggac atgcgtccgg acccagcgtc
     2461 cggcccgctc tggatcgaat tcgcttgata tcgcgtagta ttcgcggccg tcttcggcgg
     2521 cgacgccgat cgagatgagc tcgatggtgc gcccgtcgtc gatgaactcc gtgtcgtaga
     2581 agtacctcac gtcgcgccct acacagtcgc tttctcggag accggcggag tatcagtcgg
     2641 cggcgcggga gcggggtgga tctcgcgatc cagctgctcg ttgacctcgc gatcgtcggg
     2701 gaagtgcggt tcgccggcaa cgacgtgctg cagccacagc ttggcctgga ccaccggcct
     2761 gcgcagcgtg cgttcccgca acagcgcgcg gtgcatcttg cgcggcttgt tcgtgtaccg
     2821 ccagcgcgcc cacggcgcgt gcgggcgaga aagccggatc gccccgatga acagcagcgg
     2881 ggtgatgaac atgccgacaa ggccggtcca caccttgccc ttcagcagca cgatcaccgc
     2941 gagcgccagc gtcgccaccg ccagcgcgat caccaccgcc cgcgccgcca cgctgtggtc
     3001 ggaccgccag attccgacgt cgaagaacga cagtgggttg aagccgagga tcagcagtcc
     3061 ggccaccgca atcgccgcga aaaccgcgtc aaccgacgtt cgcccgtcct cggcccagta
     3121 cacgtcctga agatgcagga tcaacgcgaa ctcatcgagg accagtgccg cgccgatccc
     3181 gaaaaatgtt gccgccaccg tgaattcggg tactccaccg ttgacggcaa gcgtcaccat
     3241 ggcgatgccg gaaatcatga ccaggatcac accgatcacc acgtgatgga tgtggacgcc
     3301 tccgccggcc gagatgttgc gcggttgcca ccatctgcgg ggcttgtcgc tgtcggcgtg
     3361 gctccggatg tagcgaacga tcgtgcgggt gacgaagaaa gtcaggatga acgcaacgag
     3421 gcagaagacg agcggcagcc gcccgtgcgg gatcccgatg ccgaggtcaa acgacagatg
     3481 cacgcaaaaa actt
//
LOCUS       DASYLX010001159         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1851440,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001159 DASYLX010000000
VERSION     DASYLX010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851440"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VGP27_27460"
     CDS             complement(<1..1097)
                     /locus_tag="VGP27_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEV7855068.1"
                     /translation="MTDREAQIVDCLQQSFADLMKADPDAFRTKFRKMAADPFAFYRG
                     SACVFYADVANREDRWADERTGRVWIQGDLHTENFGTYMDGEGRLIFDVNDFDEAFVG
                     HFTWDLQRFAASVALMCWQKALSDDVIRDLITTFVRSYLDQVRWFRQTDDDSTFSLNL
                     GTARGAVLVALQDARLSIRSAMLDRITHLDGPDRRFRETSGVRRLDKAEYRKVNAAFE
                     AYQETIPKSKRFREVAYDVKDIVGRTGFGIGSAGLPSYNVLIEGFNQALDNDIVLSMK
                     QGNVAAPSRVVDDREVHDYFKHHGHRTAVSQRALQAHADPLLGYTEIDGAGFVVAELS
                     PYESDLDWSELTEPDELAEVIEQLGRAVAKV"
     gene            1295..1933
                     /locus_tag="VGP27_27465"
     CDS             1295..1933
                     /locus_tag="VGP27_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV7855069.1"
                     /translation="MLDLVLPLECGGCGAPSTRWCDACARQLRVRPDEPHLVTPRVHP
                     GAPVFSLGRYAGSRRNAIVAVKEHGRVDLIHPLGCVLRDGLRQLMTWGVLDAPLTVVP
                     APTLRRAARRRGGDPVTRIALAATCERRDVTVVRALRMTALVRDSVGLSSAARQKNVA
                     GRVRLMGPSDGMPGDVLVVDDIVTTGATASESVRVLQTSGARVVAVLALANA"
     gene            2264..>2458
                     /gene="raiA"
                     /locus_tag="VGP27_27470"
     CDS             2264..>2458
                     /gene="raiA"
                     /locus_tag="VGP27_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV7855070.1"
                     /translation="MIVEDESPAPAPSAEVVVKGRNVEIPDHFRIYVSEKLARLERLD
                     RSIYLFDVELDHERNRRQRKN"
BASE COUNT          425 a          819 c          803 g          411 t
ORIGIN      
        1 tgcaccttgg cgactgcacg gccgagttgc tcgatcacct cggccagctc gtcgggctcg
       61 gtcagctcgc tccagtccag atccgactcg tacggcgaca gctccgctac gacgaaaccg
      121 gctccgtcga tctcggtgta gccgagcagc gggtcggcgt gtgcctgcag tgcccgctgc
      181 gatacagcgg ttcggtgccc gtgatgcttg aagtaatcgt gcacttccct gtcgtcgacg
      241 acacggctcg gcgcggcgac gttgccctgt ttcatcgaga gcacgatgtc gttgtcgagg
      301 gcttggttga aaccctcgat gagcacgttg tagctcggca ggcccgcgct gccgatcccg
      361 aaaccggtcc tgccgacgat gtccttgacg tcgtaggcga cttcgcggaa cctcttcgac
      421 ttcgggatcg tctcctggta ggcctcgaat gcggcgttga ccttccggta ctcggccttg
      481 tcgagccgcc gcacgcccga ggtctcccgg aaccgtcggt ccggcccgtc gagatgggtg
      541 atccggtcga gcatcgcgga gcggatggac agccgtgcgt cctgcagcgc gacgagcacc
      601 gcaccgcggg cggtgccgag gttcaatgag aaggtcgagt cgtcgtcggt ctgacggaac
      661 caccgcacct gatcgaggta ggaccggacg aacgtcgtga tcaaatcgcg aatcacgtcg
      721 tccgacagcg ccttctgcca gcacatcagg gcgacactcg cggcgaatcg ctgcaggtcc
      781 caggtgaagt gccccacgaa ggcctcgtcg aagtcgttga cgtcgaagat cagccttccc
      841 tcgccgtcca tgtaggtgcc gaaattctcc gtatgcagat caccctggat ccagacgcgc
      901 ccggtccgtt cgtccgccca ccggtcttcg cggttggcga catcggcgta gaacacgcag
      961 gcgctgccgc gatagaacgc aaacgggtcg gcggccatct tgcggaactt ggtgcggaac
     1021 gcatcgggat cggccttcat caggtcggcg aaggactgct gcagacagtc gacgatctgg
     1081 gcctctctat cggtcattca cagggagtac ccagacgagg agccggcgaa atctgcgtgg
     1141 tggctgcgat gcctcgggct cgggcagcca tggcgcagaa gtcggcgaac accggccgac
     1201 cgagcatcct gttgaggcgc ccaggacctc gtcggcgcag aggtgacgct ggcgaaactg
     1261 tagggatgtc accgggcgcc gcgacactgg cggcgtgctc gacctggttc tgccgctgga
     1321 atgcggcggc tgcggtgcgc catcgacccg gtggtgcgac gcctgcgcgc gccaactgcg
     1381 ggtccgcccc gacgagccgc acctcgtcac cccccgggtg caccccggcg cgcccgtgtt
     1441 ctcgctgggc cggtacgcgg gcagtcgccg caacgccatc gtcgccgtca aggaacacgg
     1501 tcgtgtcgac ctgatccacc cgctgggctg cgtgctgcgc gacgggctgc gtcaactgat
     1561 gacctggggt gtgctggacg cgccgctgac cgtggtgccc gcacccaccc tccgccgcgc
     1621 cgcccgcaga cgcggcggag accccgtcac ccggatcgct ctcgctgcca cctgcgaacg
     1681 tcgcgatgtc acggtcgtcc gcgcgcttcg gatgacggcg ctggtccgcg actcggtggg
     1741 gctgtcaagc gccgcacggc agaagaacgt cgcgggccgg gtccggttga tggggccctc
     1801 ggacgggatg ccgggcgacg tgttggtcgt cgacgacatc gtcaccacgg gcgcgaccgc
     1861 ctccgagtcg gtacgtgtcc tgcaaacatc cggggcccgc gttgtcgccg tgctcgcgtt
     1921 ggcgaacgcc tgatcacagc ccctgacgac gaggccaatt tctggcagat caaaccggcg
     1981 tgacgaactg aaaacaggtg ggcgaattac tggcactagt aggtgaacac ggactacgtt
     2041 ggggccaaca tccgtgaacg cctcacggcg gcgcagaacc cgaagcgcca atcatcgccc
     2101 ggcggtagga ggtgagtatt cgacaccttg taccggtgag cggcgacata acgcgtgccc
     2161 cgtcggtgca acccctgcca ggtaggcacg cagtgcgcgt gtgacgcgaa agagaaacga
     2221 gtcgccaagc atgtcaatcc gttccatgga atcagaccgc tcgatgatcg ttgaagacga
     2281 gagccccgcg ccagcgccga gcgccgaggt cgtcgtcaag ggccgcaacg tcgagatccc
     2341 cgaccacttc cgcatctacg tctcagagaa actcgcccgc ctcgagcggt tggaccgcag
     2401 catctacctc ttcgatgtcg aactcgacca cgaacgcaat cggcgccaac gcaagaac
//
LOCUS       DASYLX010001160         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_317503,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001160 DASYLX010000000
VERSION     DASYLX010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317503"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VGP27_27475"
     CDS             complement(<1..1361)
                     /locus_tag="VGP27_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7855071.1"
                     /translation="MPEWTIGDVVDAIADVIGDRTMTVCGDRVTTYGDMAVRTRKLAN
                     FLADRGFGAHRERDGLNNWECGQDRVALIMYNDRYPEMVIGCAKARTVPVNVNHHYTP
                     AEVAELLDYVKPRAVICHRALGAKFADVLGSLNLDLLIAVDDGSDGPDIPGAVGLDDA
                     VAQGDSERRIIGSPDDLIMYCTGGTTGRPKGVLWRQSDTYLSSMVGADHDSPTEIHDK
                     VRRNEGAPWFAVSPLMHAAGLWTVFSGALAGLPVVLYDDRNNFNPALVWQTAEREKVG
                     MMTMVGDAYAAPLIEELKRTGYHLSTLYAIGTGGAATNPKHVSALLEKLPQLTIINGY
                     GSSETGNMAFGHNQRNSHRETFDLREGGTVASDDLTRFVAPGEPEVGWVVRRGRIPLG
                     YFDDADATRKTFPVIDGERVVVSGDRASLEADGTLRLYGRDSLVVNTGGEKVFVEEVE
                     AVLR"
     gene            complement(1415..>1679)
                     /locus_tag="VGP27_27480"
     CDS             complement(1415..>1679)
                     /locus_tag="VGP27_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7855072.1"
                     /translation="HFKEDPVEQLRNNVWIAPYYEDDVKLLAETIGVDKILFGSDWPH
                     GEGLADPMSFTADIPQFPEFSEEDTRKVMRDNALGLLGVNVPA"
BASE COUNT          268 a          582 c          520 g          309 t
ORIGIN      
        1 cgcagcacgg cctcgacctc ctcgacgaag accttctcac ccccggtgtt gacgacgagt
       61 gagtcgcggc cgtacaggcg cagcgtgccg tccgcctcga gcgacgcccg gtcacccgac
      121 accacaacgc gctcaccgtc gatgacgggg aaggtcttgc gggtcgcgtc ggcatcgtcg
      181 aagtagccca gcggtatccg gcctctgcgc acgacccagc cgacctccgg ctcgccgggt
      241 gcgacgaacc tggtcaggtc gtcggaggcc acagtcccgc cctcgcgcag atcgaacgtc
      301 tcgcggtgcg aattccgctg gttgtgaccg aacgccatgt tcccggtctc cgacgacccg
      361 tagccgttga tgatcgtcag ctggggcagc ttctccagca gggcgctcac atgcttggga
      421 ttggttgctg cgccgccggt tccgatggcg tacagcgtcg agaggtggta gccggtccgt
      481 ttcagctcct cgatcagcgg ggcggcatac gcgtcaccca ccatcgtcat catgccgacc
      541 ttctcgcgtt cggcggtctg ccataccagc gcggggttga agttgttgcg gtcgtcatac
      601 agcacgaccg gcagtccggc gagcgcgccg gagaataccg tccacagacc cgccgcgtgc
      661 atcagcggcg acaccgcgaa ccacggcgcg ccctcgttgc gccgcacctt gtcgtggatc
      721 tcggtcgggg aatcatggtc ggcgcccacc atcgacgaca ggtaggtgtc actctgccgc
      781 cacagcactc ctttcggccg gcccgtcgta ccacccgtgc aatacatgat gagatcatcc
      841 ggggatccga tgatcctccg ttccgaatca ccctgcgcga cagcatcgtc gagtccgacg
      901 gcgcccggga tgtcggggcc gtcgctgccg tcgtcgacgg cgatcagcag atcgagattc
      961 agcgatccca gtacgtcggc gaacttcgcc ccgagggcgc ggtgacagat gaccgcccgg
     1021 ggtttgacat agtcgaggag ttcggcgacc tctgccgggg tgtagtggtg attgacgttc
     1081 accgggaccg tgcgggcctt ggcgcagccg atcaccatct cgggatagcg atcgttgtac
     1141 atgatcaacg cgacgcggtc ctggccgcac tcccagttgt tcagcccgtc gcgctcgcga
     1201 tgtgcgccga agcccctgtc cgccaagaag ttcgccaact tacgcgtgcg caccgccatg
     1261 tcgccgtagg tggtcacgcg gtcaccgcag accgtcatcg tccggtctcc gatgacgtcg
     1321 gcgatggcgt cgacgacatc gcctatggtc cactcaggca ccctgggtgc tcctcacgtc
     1381 cgccagacac ccctcgctcc tcacgtccgc ggtctcatgc cgggacgttc acgccaagta
     1441 ggccgagggc gttgtcccgc atcaccttcc gggtgtcttc ctcgctgaac tcggggaact
     1501 gtggaatgtc ggcggtgaac gacatcggat ccgcgagccc ctcgccgtgc ggccagtccg
     1561 agccgaacag gatcttgtcc acgccgatcg tctcggcaag cagcttcacg tcatcctcgt
     1621 agtacggcgc gatccacacg ttgtttcgca gctgctcgac cgggtcttcc ttgaagtgg
//
LOCUS       DASYLX010001161         7111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1851888,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001161 DASYLX010000000
VERSION     DASYLX010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851888"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGP27_27485"
     CDS             <1..238
                     /locus_tag="VGP27_27485"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855073.1"
                     /translation="RTLSEPRGFMKMLIAADGAEILGFTVFGAEASELMAAVQTAMIG
                     RVPYTALRSAIFAHPTTAEGLTFLLRSTPTTPAR"
     gene            525..5954
                     /locus_tag="VGP27_27490"
     CDS             525..5954
                     /locus_tag="VGP27_27490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7855074.1"
                     /translation="MGAGGVLPDRDPPVRAEILHESERTRVTRLFFPGRSVIHKEPLG
                     PDAQRRLQHEVAMLERVRGVVGVAQLLDAPRYPGSIVVEDVGGMSLAGQVKPLAVDDL
                     TAFAVELARAVAGVHGRGVLHRDITPANIVICGGGGVPCLVDFALATSFAEVRPEFTH
                     HTQIVGTLAYLAPEQTGRTGRSVDQRADLYALGATLYEWATGEPPFGSGDPLRLTYDH
                     LARVPVPPAEVNPAVPGLLSEIIMHLLEKEPDNRYQTADGVIYDLERLRDAQARPAAA
                     PVGVGEHDFPLRLLPPSRLVGRDDEVAALQAAFEDALVGRCCGVVVSGAPGVGKTALV
                     DELRPVVTGRDGWFVAGKFDQYRRVVEFDAGYQGFRALGRLLLAEPEDELAEVRERIL
                     RAIGPNAGLFTAVLPEFAALLAVPPNPGDPLTAQVRAQRAGVQVLRAVASRKRPVLFF
                     VDDLQWAGRTPLGFVDLVLSEEPIDGLLLVGAYREDDIDAAHPLAAPLSRWRDQTGVQ
                     HLRLENLPVASLVTLVAEMLHADRATAAALVRVIEPHTCGNPYETVELLNLLRHDGVL
                     TATADGWRWDNAVVRGHLGQYEVAGLLAARVDAMAAQARHMVEAMAALGGRVEVSVLQ
                     AATGEPASGVEQRLAPALGDGLLVMEPGLHQAVRFRHDRIREVILRGLDPQRRHTLQW
                     AMARRLAAVPELFAIAAEQYLPVVDAVDDAAERSQVVGLLRRAADQAALIGDYALVNA
                     LLVAALQLIDPGDTATLVEVHTGRHATLYSIGRLDEADEEYRTITRLCRTALQRADAT
                     VVQVHSLTHRKRSAEAIELGLDSLRECGITVPAADRLPAELDGQFDHLYRWLDHTEAA
                     DDLARPDITHPTLLATTRLLNAVLPAAYLVPAPALHAWLSLEALRIWHEHGPGPTLLG
                     PAGHTATAAMALRGDSAAAYRAIRRILALGEARGYEPDTSQARFLFAILSCWFEPIEN
                     GVQAAQRAREGLIAGGDLAHAGYAYIPSVQSLLDCTPSLDSYATEVEAGLAFVRRTGS
                     EQIGQWLDSYRWLAGVLRGESSAATDEAVPVDRYADNPLALLFTYINRAIAAAIFGDL
                     GGLARHTAAAMPLLPAALGQYLTAVAHLLRGLALAGQARTADGDERGGLLSELDDVTQ
                     WLAARAANAPTNFLHLLRLVEAERAWTVGDFRAAILAFDAARREVAQRQRPWHRALIA
                     ERAARFYLAHGVEHAGYDLLAQGRQDYLAWGATAKADQLDWAYPTLRPQAGAIALNSG
                     DQPADLPHRRTTVTTGTIDLLGIVSASQALSSQTSIEGLHSRVVEVLSAMTGATGVHL
                     VPWSDDRQDWLLTAPGAGTVPLTVLRYAQRMREPLVVGDATRDDRFARDPYFIDLTCC
                     SLMALPILSRGTLRAVLLLENRLIRGAFTTQRLDTVNLIAGQLAVSLDNAQLYTEFRR
                     IADEQAALRGVATLVARGVGPDLVFAAVAQEVSALFGADAAAIMRFEPDGEATVMGGC
                     GFEHSQPGSRGRPNPHLAMASVQATGRAARRDMDDPTASLPETTPEAVRSAVASPIVV
                     EGRVWGAIGVGLRRERLPQDTEQRLAEFTELVATAIANAESRAELTTSRARIVATADQ
                     TRRRIERDLHDGAQQRLVSLALHLRATQAAVSPELGEFGAELNRAVAEATGVLDELRE
                     IARGIHPAILAQGGLGAALRSLARRSPIPVDLDMRLQGRLPEHVEVSAYYVIAEALTN
                     TAKHARASAVTVTVEAADAVLRIAVRDDGDGGADFAHGTGLVGLKDRVEALGGRTFLD
                     SPRGAGTSLRVELPLSTENGGVSPR"
     gene            5992..6255
                     /locus_tag="VGP27_27495"
     CDS             5992..6255
                     /locus_tag="VGP27_27495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855075.1"
                     /translation="MDARRDDTGDQMLEYRRGYHGAVSAAQRRRISLTVYGISPQSLS
                     RMETAFATTSTLSKGTDSLTAVFTPDEADRLRTFHVTTGIADG"
     gene            6615..7010
                     /locus_tag="VGP27_27500"
     CDS             6615..7010
                     /locus_tag="VGP27_27500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855076.1"
                     /translation="MASRYPFIFVDQAMQNRSPLDPFIAELCHRVGRLRGAKVTGTVR
                     PSTVVVANVLPEHHPQMPLPEDQHADGEFGSDSSDETFGETVRPRATGRNPDHLDAHI
                     GQDSIERRGELAARSQTRNRNWVDFRFNV"
BASE COUNT         1093 a         2345 c         2524 g         1149 t
ORIGIN      
        1 caggactctg tccgagccgc gcggttttat gaagatgctg atcgctgccg acggtgccga
       61 gattctcggg ttcaccgtgt tcggggcgga ggccagcgaa ctgatggccg cggtccagac
      121 ggcgatgatc ggccgcgtcc cgtacacggc actgcggagt gcgattttcg cccatccgac
      181 aacagctgaa gggctcacct tcttgctgag gagcacgccc accaccccgg cccgttagcg
      241 tctcttggtg ctgtatcggt atacggcacc gtgccacgag cgctcgtcac ctgcgctatt
      301 agcggcggga ccggaattgg aagcgctgag ccaggggcgc cccgatctcg gggcttccaa
      361 cggcgttgtc atccctgaaa gggacgacga tgcccggctc gccagcctct ctgcgggccg
      421 gtggcttcgc ggcgggtcac gttctgatga atagtcgcct tggccgtcga ataggggtgt
      481 aggcacggta tgaggtgggg tccgaccgtg cgtggaggtg tcggatgggg gcaggcggtg
      541 tgctgccgga ccgagatccg cctgttcgtg ccgaaatact gcacgagagc gagcgcacgc
      601 gggtgactcg gctgttcttc cctgggcgct ccgtcatcca caaggagccg ctggggccgg
      661 acgcgcagcg gcggctgcag catgaggtgg cgatgctgga acgggtgcgc ggtgtggtgg
      721 gggtcgcgca gttgctggat gcgccgcggt acccggggtc gatcgtggtg gaggacgttg
      781 gtggcatgag ccttgcgggg caggtgaagc cgctcgctgt ggatgatctg accgcgtttg
      841 cggtggagtt ggctcgggcg gtggcggggg tgcacggccg gggggtgctg caccgggaca
      901 tcactccggc gaacatcgtg atctgcggcg gcggcggtgt cccgtgcctg gtggacttcg
      961 cgttggcgac gtcgttcgcc gaggttcgtc ctgagttcac gcaccacact cagatcgtgg
     1021 ggacgttggc gtatctggcg ccggagcaga ccgggcgtac cggtcggtcg gtggatcagc
     1081 gtgccgatct gtatgcgttg ggtgcgacgt tgtatgagtg ggcgacgggt gagccgccgt
     1141 tcggttccgg ggacccgttg cggctcactt atgatcattt ggctcgcgtg ccggtgccgc
     1201 cggctgaggt gaatccggct gtaccggggc tgctgtccga gatcatcatg catctgctgg
     1261 agaaggaacc ggacaaccgg tatcagacgg ccgacggggt gatctatgac ctggagcgat
     1321 tgcgggatgc ccaggcacgt ccggcagcgg ctccggtcgg cgttggcgaa catgattttc
     1381 cgttgcggct gctacctccg tcgcggcttg tgggccgcga tgacgaggtg gcggcactgc
     1441 aggcggcgtt tgaggatgcg ctggtgggcc ggtgttgtgg ggtggtggtc agcggggcgc
     1501 cgggggtggg taagacggcg ttggtcgatg agctgcggcc ggtggtgacg ggccgggatg
     1561 gctggttcgt ggcgggcaag ttcgatcagt atcggcgggt tgtggagttc gacgcgggat
     1621 atcaggggtt tcgcgcgttg ggtcggctgt tgctggccga gccggaggat gagctggccg
     1681 aggtccgcga gcggatcttg cgggcgatcg gcccgaacgc gggtctgttc accgcggtgc
     1741 tgccggagtt cgcggcgctg ctggcggtgc cgccgaatcc gggggatccg ctcacggcgc
     1801 aggtccgggc gcaacgagcg ggtgtgcagg tgttgcgggc ggtcgcttcg cggaaacggc
     1861 cggtgctgtt cttcgtcgat gatctgcagt gggcggggcg cacgccactc ggcttcgtcg
     1921 acctcgtgct gagcgaagag ccgatcgacg gcctgctgct ggtgggtgcc tatcgcgaag
     1981 acgacataga cgcggcgcat ccgttggcgg cgccactgtc ccgatggcgt gaccagaccg
     2041 gagtgcagca cctgcggctg gagaacctgc ccgtggcgag tctcgtcacc ctggttgccg
     2101 agatgctcca cgcggaccgg gccactgcgg cggccctggt ccgggtgatc gaaccgcata
     2161 cctgcggcaa tccgtacgag actgtggaac tgctcaacct gttgcggcac gacggcgtgc
     2221 tgacggcgac ggccgacggg tggcggtggg acaacgcggt ggtgcgtggt catctgggcc
     2281 agtacgaggt tgccggcctg ttggcggctc gggttgacgc catggcggca caggctcggc
     2341 acatggtgga ggcaatggcg gctcttggcg gccgagtcga ggtgagcgtg ctgcaggccg
     2401 cgaccggtga accggccagc ggggtggaac agcggctggc gccggccctg ggggatggcc
     2461 tcctggtgat ggagcccggg ctgcatcagg cggtgcggtt tcgccacgac cgcatccgcg
     2521 aggtgatcct gcgcgggctg gaccctcagc gacgacacac cctgcagtgg gcgatggcac
     2581 ggcggcttgc ggcggtgccg gagttattcg cgatcgcagc ggagcagtat ctgccggtgg
     2641 ttgatgcggt cgacgatgcc gcagagcgaa gccaggtggt ggggctgctg cggcgcgccg
     2701 ctgaccaggc ggctttgatc ggggactacg cgctggtgaa cgctctgctg gtcgccgcgt
     2761 tgcaactgat cgaccccggt gacaccgcca cgcttgtcga ggtgcatacc ggccgacacg
     2821 ccacgctgta cagcatcggg cgcctcgacg aggcggacga ggaataccgc acgatcacgc
     2881 gactgtgccg caccgccctg cagcgcgccg acgcgacggt ggtgcaggtg cacagcctca
     2941 cccaccggaa acgatctgcg gaggcgatcg agctcggcct ggactcgctg cgcgagtgcg
     3001 gcatcaccgt accggccgcc gaccggctcc ccgccgagct cgacggccag ttcgaccatc
     3061 tgtaccggtg gctcgaccac accgaagccg ccgacgatct ggctcggccc gacatcaccc
     3121 acccgacact gctggccact acccgtctgc tcaacgcggt cctgccagcg gcctacttag
     3181 tccccgcccc cgccctgcac gcgtggctga gcctggaagc gctgcggatc tggcatgagc
     3241 acggcccagg ccccaccctg ctcggcccgg ccggtcacac cgctacggcc gctatggcgc
     3301 tgcgcggcga ctccgctgcc gcataccggg caatccggcg gatcctcgcg ctgggcgaag
     3361 cccgcggcta cgagcccgac acctcgcagg cgcgcttcct gttcgcgatc ctcagttgtt
     3421 ggttcgagcc gatcgaaaac ggtgtccaag cagcgcagcg ggcccgagaa gggctgatcg
     3481 cgggtggcga cctggcccac gccggctacg cgtacatccc gagcgtgcag tccttgctgg
     3541 actgcacgcc ctcgctggac agctacgcca ccgaggtcga ggcggggctg gccttcgtgc
     3601 gccgtaccgg cagcgaacag atcggccagt ggctcgacag ctaccggtgg ctggctggtg
     3661 tgctgcgcgg cgaaagctcc gcggcaaccg atgaagcggt ccccgtcgac aggtacgccg
     3721 acaacccgct ggcgcttctg ttcacgtata tcaaccgtgc gatcgccgcc gccatcttcg
     3781 gggatctggg cggcctggcg cggcacaccg ccgcggcgat gccgctgcta ccggctgcct
     3841 tgggccaata cctgaccgcc gtcgcccacc tgttgcgcgg gctcgccctc gccgggcagg
     3901 cccgcaccgc agacggtgac gagcgcggcg gtctgctgtc cgaactagac gatgtgacgc
     3961 agtggctggc cgcccgcgca gcgaacgcgc cgacaaactt cctgcacctg ctgcggttgg
     4021 tcgaggccga gcgggcgtgg acggtcggcg acttccgcgc cgccatcctc gccttcgacg
     4081 ccgcacggcg cgaggtcgcc cagcgccaac gcccgtggca tcgggccctg atcgccgaac
     4141 gcgccgcccg cttctatctc gcccacggcg tcgaacacgc cggctacgac ctcctcgccc
     4201 agggccgcca ggactacctc gcgtggggcg cgaccgcgaa agccgaccag ctggactggg
     4261 cctacccgac cctgcggcca caagccggcg cgatcgccct aaacagcggc gaccagcccg
     4321 ccgatcttcc ccaccgtcgc accacggtca cgaccggaac gatcgacctg ctcggcattg
     4381 tgtccgcgtc gcaggcgctg agctcccaga ccagcatcga agggctgcac tcgcgcgtgg
     4441 tcgaggtgct cagcgcgatg accggagcca ccggcgtaca cctggtgccg tggagcgacg
     4501 accggcaaga ctggctgctg accgcacccg gcgccggcac cgtgccgctg accgtgctgc
     4561 gatacgccca acggatgcgt gagccgctgg tcgtgggcga cgccacccgc gacgaccgct
     4621 tcgctcgcga cccatatttc atcgacctca cctgctgctc actgatggcc ctacccatcc
     4681 tcagccgcgg cacactacgg gcggtgctgc tgctggaaaa ccgcctcatc cgcggcgcgt
     4741 tcaccaccca acggctcgac actgtcaacc tcatcgccgg ccaactcgcc gtctcgctcg
     4801 acaacgccca gctgtatacc gagtttcgcc ggatcgccga cgagcaggcg gccttacggg
     4861 gggtggccac gttggtcgcc cgcggggtag ggccggatct cgtgttcgcc gcggtggccc
     4921 aggaggtcag cgcgctcttc ggcgccgacg ccgcggcgat catgcgattc gagccggacg
     4981 gggaggcgac ggtgatggga ggctgcgggt tcgagcactc ccaaccgggt tcacgcggca
     5041 ggccgaaccc ccacttggcc atggcatcgg tccaggcgac gggccgcgcg gcgcgccgcg
     5101 acatggacga cccaacggcg agcctgcccg agaccactcc cgaggcagtc cgctccgcgg
     5161 tggctagccc aatcgtggtc gagggtcggg tctggggcgc aatcggcgtc gggttgcggc
     5221 gcgaacggct gccccaagac accgagcagc ggctggccga gttcactgag ctcgtcgcga
     5281 ccgccatcgc caacgctgag agtcgggccg aactcaccac gtctcgcgcc cggatcgtcg
     5341 ccaccgccga ccagacgcgc cgacgaatcg agcgggacct gcacgacggc gcccagcagc
     5401 ggctggtctc gctggcgcta cacctgcgcg ccacgcaggc agcggtatcg cccgagctcg
     5461 gcgagttcgg cgcggagctc aaccgtgccg tcgccgaggc gaccggcgtg ctggacgaac
     5521 tgcgcgagat cgcgcgcggc atccaccccg cgatcctggc ccagggcggc ctcggcgccg
     5581 cgctgcgctc gctcgcccgc cgctcaccga tcccggtcga cctcgacatg cgcctgcagg
     5641 ggcggctgcc cgagcatgtc gaggtcagcg cgtactacgt cattgccgag gcgctaacca
     5701 acacggccaa gcacgcgcgc gcctccgccg tcacggttac cgtcgaagcc gccgacgccg
     5761 tcctgcgcat cgcggtccgc gacgacggcg acggcggcgc cgacttcgcc cacggcaccg
     5821 gcctggtcgg cctcaaggac cgcgtggagg cgctcggcgg ccgaaccttt ctcgacagcc
     5881 cacgcggggc gggaaccagc ctgcgcgtgg agctcccgct cagcacagag aacggcggcg
     5941 tcagcccccg ctagcccgag acccgagact cccaacggtt cagagccgca ggtggacgcc
     6001 cgccgtgacg acacgggcga ccagatgttg gagtaccggc gcggatacca tggcgcggtc
     6061 agcgcggccc agaggcggcg gatttcgttg accgtgtacg ggatcagccc gcagtcgttg
     6121 tccagaatgg aaactgcctt tgccaccacc tcgacgctga gcaagggcac ggactcactg
     6181 accgcggtgt tcacccccga cgaagccgac aggcttcgga ccttccacgt caccaccggt
     6241 atcgcagacg gttaacacgc tgttcggatt cggtcccgaa gctcgtgtcc gcgcaacagg
     6301 tgtctcacaa ccaggtttct gctggacacg gcttttgacg acgagctgga cgaggtcggt
     6361 gaacgttcga agggatcgaa tctcggctgt ggaacaccca tatagggttc ccacaagatg
     6421 gtgcagccgt gtatctactg cgagcttccg aaggcgaaaa accaggagca tgtcctggca
     6481 tcgtggatca tcgaggttct ctcctaggac acgcgcagtc tcccgctccg gctgcggatg
     6541 gagctgtcgg atcagagcgg tggcaagcgg gtgactcttg tggggttcca gaagtcggtc
     6601 gcccatggta agcagtggct tcacgctacc cgttcatatt cgttgatcaa gccatgcaga
     6661 accggtcgcc gcttgatccg ttcatagctg agctctgcca cagggtgggt cggttgcggg
     6721 gggcgaaggt tacgggcacg gtgaggccgt cgaccgttgt agtggcgaac gtactccccg
     6781 agcaccaccc gcagatgccg ctccccgaag atcaacatgc ggacggtgag ttcggctctg
     6841 acagttccga cgaaacgttc ggcgaaacag ttcgcccgcg ggcaacgggg aggaatcctg
     6901 accacctgga tgcccacatc ggccaggaca gcatcgaacg acgcggtgaa ctggccgccc
     6961 ggtcgcagac gaggaaccgg aactgggtcg acttccgctt caacgtgtag tggaaagcga
     7021 aacttcaact atcgatgcgc ggggagacca tggcccgggc gccgcgccgg gagggtctcc
     7081 cgcatgcgcg gcaggcgttt gctgggtcaa g
//
LOCUS       DASYLX010001162         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3716339,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001162 DASYLX010000000
VERSION     DASYLX010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3716339"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VGP27_27505"
     CDS             complement(<1..634)
                     /locus_tag="VGP27_27505"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7855077.1"
                     /translation="MTREPRIVIIGAGVAGIATAVTLQRAGFHDFTILEKGNDVGGVW
                     HWNRYPGLTCDVPSQLYQFSFAPKPDWTGIFAPGEEIQRYLGEVVEQFGLNDRLRLNS
                     EVVSTVFTGTAWHVVTADDTEAEADFVLAAATGVLHHPFTPNIPGLDSFGGDVVHTAR
                     WNRAFETGGRRVAVIGNGSTGVQIVSALQRDAAHIAHFVRSPQWVIWAPMS"
     gene            complement(631..1488)
                     /locus_tag="VGP27_27510"
     CDS             complement(631..1488)
                     /locus_tag="VGP27_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7855078.1"
                     /translation="MNLDGVRMTYPVIDLDGAIVAVTGGARGIGKSTADLFSARGATV
                     CVGDLDGAQCSPAPRVRGYTVDVTSRNSFAEFVTAVIERHGRIDVLVNNAGVMPLGDF
                     LSEDDAISRTTFEVNVWGLIHGMRLVLPHMIARGHGHIVNVASMAGKLPIPGMAVYNA
                     SKFAAVGLSAAVREEYRDSGVSVTTVLPSAVRTRLASGVPLGRGLPTVEPEAVARAIV
                     GSVASRRAQITVPRYLAGWDLLSAVTPPAVIGLARRAIRDRRALTSVDHELRDGYQKT
                     IAAQAGGRR"
     gene            1542..>1703
                     /locus_tag="VGP27_27515"
     CDS             1542..>1703
                     /locus_tag="VGP27_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7855079.1"
                     /translation="MARMTRAESQAQTRAQLVATARRMFFEDGYHPTSLEKVADAAGY
                     SKGAVYSNFR"
BASE COUNT          315 a          559 c          545 g          284 t
ORIGIN      
        1 ggctcatcgg cgcccatatc acccactgcg gggaccgcac gaagtgagcg atgtgcgcgg
       61 cgtcgcgctg cagtgcggac acgatctgta cgccggtgga cccgtttccg atgacggcga
      121 ctctgcggcc gccggtctcg aatgcgcggt tccaccgcgc ggtgtgcacg acgtcgccgc
      181 cgaacgagtc gagtcccgga atgttcggcg tgaacgggtg atgcaaaaca ccggtcgctg
      241 ctgccaacac gaaatccgct tctgcttccg tgtcgtccgc ggtgacgaca tgccaagcgg
      301 taccggtgaa gaccgtggag acgacttcgg aattcaaccg cagccgatcg tttaggccga
      361 actgttcgac gacctcgccg agatagcgct ggatctcttc accgggcgcg aatatgccgg
      421 tccagtccgg tttgggagcg aatgagaact ggtagagctg cgacggcacg tcacacgtca
      481 gacccggata acggttccaa tgccagacgc cgccgacgtc gttgcccttc tccagaatgg
      541 tgaagtcgtg aaagcccgcc cgctgcagcg tgactgccgt ggcgatgcct gcgacacccg
      601 cgccgatgat gacgatccgc ggttcccggg tcatcgtcgg cctccggcct gcgccgcgat
      661 cgtcttctgg tagccatcgc gcaactcgtg gtcgaccgat gtcaacgccc ggcggtcgcg
      721 aattgccctg cgggccaagc cgatcacggc tggcggagtc acggcgctga gcaggtccca
      781 tcccgcaaga taccggggca ccgtgatctg ggcgcgcctg ctggcgacgc tgccgacgat
      841 ggcccgcgcg acggcctccg gttcgacggt cggtagcccc cgcccgagtg ggacaccaga
      901 tgcgagccgg gtgcgcaccg cgctgggcag cacggtcgtc acactgacac ccgaatcgcg
      961 gtactcctcg cggaccgcgg cggacaaccc cacagcggcg aacttactgg cgttgtacac
     1021 cgccataccg ggaatcggca gctttccggc catcgaagcg acattcacga tgtggccgtg
     1081 cccgcgtgcg atcatgtgcg gcaacacgag tcgcatgccg tggatcaacc cccagacgtt
     1141 gacctcgaat gtcgtcctgc ttatcgcgtc gtcctcggac agaaagtcgc cgagcggcat
     1201 gacgccggcg ttgttgacga gaacatcgat acggccgtga cgttcgatca cggcggtgac
     1261 gaactcggcg aacgagttcc gcgatgtcac gtcgacggtg tagccgcgca cgcgtggagc
     1321 aggcgagcat tgagcaccgt cgagatcgcc gacacagact gtcgcccctc gggcgctgaa
     1381 gaggtccgcc gtcgacttcc cgatcccgcg ggctccgccc gtgacggcga cgattgcacc
     1441 gtcaagatcg ataacggggt aggtcatcct gactccgtcc agattcaaaa actgacggta
     1501 tctggaatct gtaggtatgt caaaggggct agaatgcagc catggcccgc atgacccgcg
     1561 ctgagagcca ggcccagacc agagcgcaac ttgtcgcgac cgcgaggcgg atgttcttcg
     1621 aggacggcta tcacccgacg tcactggaga aggtcgccga cgccgccggc tactcgaaag
     1681 gcgcggtgta ctccaacttc cgc
//
LOCUS       DASYLX010001163         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3500519,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001163 DASYLX010000000
VERSION     DASYLX010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500519"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VGP27_27520"
     CDS             complement(<1..1420)
                     /locus_tag="VGP27_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HEV7855080.1"
                     /translation="MRVDGRDITVSGSLLQPLTRRTSDILRLLLAAVFLAVVITSSLI
                     TRYDWVSLERSISDIVGVLTPTQSNLVYLAYGIAILVLPFAILIGLIGGRHWKLLGAY
                     AAAGVIALVALSVRPGGFAAPRWHWDLADQPKTFLSQFLDDPRWIAMLAAMLTVSGPW
                     LPARWRHWWWALLLAFVPIHLVLSAVVPARTLLGLAVGWFVGALVILVVGTPALEVPL
                     EGAVRAMGRRGCSVTGLTVLRRAGRGSLLLSAACADGATKELELYSPNQRSGGALRQL
                     WRKFTLRGGETAPFHASIRRVVEHRALMAIAIGDLGVANTTPLAVTPLDRGWTLYAHT
                     PARGTPLDEATETTPVPRTWGSLRTLHDQDISHGDLRANKITVDGDTVMFGGFDHAEY
                     GATDTQLQSDIAQLLVTTSALYDTPTAVRAAMGVFGKEAVLTASRRLTKAAVPARIRE
                     PIHNAKAVLGAVREEVRLQTGAD"
     gene            complement(1444..2406)
                     /locus_tag="VGP27_27525"
     CDS             complement(1444..2406)
                     /locus_tag="VGP27_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV7855081.1"
                     /translation="MKILVADANLVPHRERFEAALPDVRVCWQANGVSSDDLRDADVY
                     VGSRFTSAMANTAEELKLIHVAGAGTDKVDFDALPPDMLVANTFHHEQSIAEYVAAAA
                     VMLRRGFLTQDRELRRNIWATSVYDAAIPQPRALGGAHIGFVGFGHIGRRAWNLLRAF
                     GCTGAAVTGSGRVADVGLSWAADTGELDRLLGECDVAVVSAPLNEHTEGMIGAAQLTA
                     LGADGVLINVGRGPLVQEPALYDALAGGVIRAAAIDVWYRYPAGDDVSPPADLPFAEL
                     PNLLMTPHSSGVTRDTFTGRVDDVVANIGRLKRGEPLRNVVHGG"
     gene            complement(2420..3766)
                     /locus_tag="VGP27_27530"
     CDS             complement(2420..3766)
                     /locus_tag="VGP27_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV7855082.1"
                     /translation="MTVPVVREMTVIPIAGHDSILLNLSGAHGPFFTRNLVIITDSDG
                     NTGVGEVPGGEPIRRTLEDARSIVTGRSIGDHHAVLNDMRRAFGGRDAGGRGAQTFDL
                     RVAVHAVTAVESALLDLLGRHLGVPVAALLGDGKQRNRVQALGYLFFVGDRNKTDLAY
                     RSPADEDASDDPADEWLRVRHEEALSPEAIVRLAEAAKARYGFADFKLKGGVLPAPDE
                     AKAVIALADRFPEARITLDPNGGWLLDDAVKTCRELADVLAYAEDPVGPEAGFSGREV
                     MAEFKRATGLPTATNMIGTDWRELGHAIRANAVDIPLADPHFWTMAGSVRVAQLCDAW
                     GLTWGSHSNNHFDVSLAMFTHVAAAAPGAITAIDTHWIWQDGQRITKEPYGITDGYLT
                     VPDEPGLGVELDDEAVAAAHELYKREGLGSRDDAVAMQYLIPGWEFDSKRPALQRD"
     gene            complement(3783..4616)
                     /locus_tag="VGP27_27535"
     CDS             complement(3783..4616)
                     /locus_tag="VGP27_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7855083.1"
                     /translation="MTDEPLPDVAGSTAPDLAVRKVKSAVRTVELLEYLAARHDNPAR
                     LREISDALDMPRSSAHALLRTLVAQGWVRSDASGTLYGIGIRALLVGTSYLDSDPYLP
                     LITPFLDDLRQDLDETFHLGRLDGTDIVYLATRESTQYNRIQSRVGRRLPAYATALGK
                     SLLAERFGVERDAHLPAALTPLTTETIVDRDVFNEALEEVRVRGYATDDQENTTGLRC
                     FAVALRYCSPAQDAISASVPVERLNLQREREIADALRTMGDKVSRVVRPLANGDKWFA
                     S"
     gene            complement(4618..5346)
                     /locus_tag="VGP27_27540"
     CDS             complement(4618..5346)
                     /locus_tag="VGP27_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7855084.1"
                     /translation="MSPMGYCIVAGTVMLASALQASIGFGIGLLAAPIVALVDPRLIP
                     GTLIMVATLLTLMVVIREREDIDLRGTGWALVGRIPGTIAGAVLLAMLPHRALAILLA
                     AVVLSGVVLTSFGWIPAPRRRNVVLAGAASGVLGTATAIGGPPMALVWQRNTGARLRG
                     TMSGFFLVGSIMSIAALAVTGSIDERTMRGFAVLIPAAIAGYLLSRPMNRFLDPQRLR
                     WLAVGVSTVGAVVLIGRELLAIGG"
     gene            complement(5343..>5553)
                     /locus_tag="VGP27_27545"
     CDS             complement(5343..>5553)
                     /locus_tag="VGP27_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7855085.1"
                     /translation="QRRERILLEGEVPSPLNPPSGCRFRTRCWKATEVCATTAPPSAL
                     DLEVVGHTAECHHPLLEEGSVLASA"
BASE COUNT          899 a         1890 c         1908 g          856 t
ORIGIN      
        1 catccgcacc ggtctgcagt cgcacctctt cgcgcacagc accgagcacc gccttggcat
       61 tgtgaatcgg ctctcgaatg cgcgcgggca cagcagcttt cgtcagtcgc cgcgacgccg
      121 tcaggacggc ttccttgccg aagacgccca tcgccgcgcg caccgccgtc ggcgtgtcgt
      181 acagcgcgct cgtcgtcacc agcagctgcg cgatgtccga ctgcagttgt gtgtcggtgg
      241 ctccgtactc ggcgtggtcg aaaccgccga acatcacggt gtcaccgtcg acggtgatct
      301 tgttggcgcg caggtcgccg tgggagatgt cctgatcgtg aagggtccgc agcgatcccc
      361 aggtccgcgg caccggcgtc gtctcggtgg cctcgtccag cggtgtgccc cgggcaggcg
      421 tgtgcgcgta aagcgtccac cctcgatcga gcggcgtgac ggcgagcggc gtggtgttgg
      481 ccactccgag atcaccgatg gcgatcgcca tcaacgcgcg gtgctcgacg acgcggcgta
      541 tcgacgcatg gaacggcgcg gtttcgccgc cgcgcagggt gaacttccgc cagagctggc
      601 gcagcgcccc tccgctgcgc tggttcgggc tgtacaactc gagttccttg gtggcaccgt
      661 cggcgcacgc cgcggagagc agcagcgacc cgcgtccggc gcgccgaagc acagtcagcc
      721 cggttaccga gcagcctcgc cggcccatcg cccgcacggc gccctcgagc ggaacctcca
      781 gggcgggcgt gccgacgacg agaatcacca gcgcgccgac gaaccagccc accgcaagac
      841 cgagcagtgt gcgggcgggc accacggcgc tgaggacgag gtggatgggc acgaaggcca
      901 acagcagcgc ccaccaccaa tgccgccacc gcgcgggcag ccagggaccc gaaacggtga
      961 gcatcgctgc gagcatcgcg atccagcgcg gatcgtcgag gaactgcgag aggaaggtct
     1021 tgggctggtc ggccaggtcc cagtgccatc gcggcgccgc gaagcccccc ggccggaccg
     1081 acaatgcgac cagcgcgatc acaccggccg ccgcgtaggc gcccagcagt ttccagtgcc
     1141 tgccgccgat cagcccgatc aggatcgcga atggcagcac caggatcgct atgccgtagg
     1201 cgaggtaaac cagattggac tgggtgggag taaggacgcc gacgatgtcg gagatcgacc
     1261 tctccagcga cacccagtca tatcgggtga tcagcgagct ggtgatgacg accgcgagga
     1321 agacggccgc cagcagcagc cggaggatgt cgctggttcg gcgggtcagc ggctgcagca
     1381 agctgcccga aacggtgatg tcccggccgt cgactcgcac gccgactagt taacccgaca
     1441 ggctcagccg ccatgcacga cattacgcag cggttcgccg cgcttcagcc tgccgatatt
     1501 ggcgacgacg tcatcgacgc ggccggtgaa ggtgtcgcgg gtgactcccg acgaatgcgg
     1561 tgtcatcagg agattcggca gttcggcgaa tggcagatct gcgggcgggc tgacgtcgtc
     1621 accggctgga tagcggtacc acacgtcgat cgcggcggcc cggatgacgc cgcccgcaag
     1681 cgcgtcgtac agcgcgggct cttgcaccag cggaccgcgg ccgacgttga tcagcacacc
     1741 gtcggcgccg agtgctgtca gttgcgcggc accgatcatg ccttcggtgt gttcgttcag
     1801 cggtgcggac acgacagcga catcgcattc acccagcaac cggtccaact cgccggtgtc
     1861 ggccgcccaa gacaggccga cgtcggccac tcggcctgag ccggtaaccg cggcgccggt
     1921 gcagccgaag gcacgcaaca gattccacgc gcgccggccg atgtggccga agccgacgaa
     1981 gccgatgtgg gcgccaccga gcgccctggg ttgcggtatc gccgcgtcgt acaccgacgt
     2041 cgcccagatg ttgcggcgca gttcccggtc ctgcgtgagg aagccgcggc gcagcatcac
     2101 cgcggcggcg gcgacgtatt ctgcgatgga ctgctcgtgg tgaaaggtgt tggccaccag
     2161 catgtcgggc ggtagcgcgt cgaagtcgac cttgtcggtg cccgcacccg ccacgtggat
     2221 cagcttgagc tcctccgcgg tgttcgccat cgccgaggtg aagcgactcc cgacatagac
     2281 gtcggcgtcc cgcagatcgt cggatgacac gccgttcgcc tgccagcaca cccgcacgtc
     2341 gggcagcgcg gcttcgaacc gctcgcggtg cggaaccagg ttggcgtcgg caactagaat
     2401 cttcagctat cttctcctgc taatcccgtt gcagcgctgg gcgcttgctg tcgaattccc
     2461 atccgggaat caggtactgc atggcaacgg cgtcgtcgcg gctgccgagt ccttcgcgtt
     2521 tgtagagctc gtgtgcggcc gcgaccgcct cgtcgtcgag ctccacgccg agaccaggct
     2581 catcgggcac ggtgaggtag ccgtcggtga ttccgtacgg ctccttggtg attcgctgtc
     2641 cgtcctgcca gatccagtga gtgtcgatcg cggtgatggc gccgggcgcg gcggcggcca
     2701 cgtgggtgaa catcgccagc gacacgtcga agtggttgtt ggagtgtgaa ccccacgtca
     2761 atccccacgc gtcgcacagc tgcgcgacgc gcaccgagcc ggccatcgtc cagaagtgcg
     2821 gatcggccag cgggatgtcc acggcgttgg cgcggatcgc gtggccgagc tcccgccagt
     2881 cggtgccgat catgttcgtc gccgtcggga gcccggtggc ccgtttgaac tccgccatca
     2941 cctcgcggcc ggagaagccg gcctcggggc cgacgggatc ctcggcgtag gccagcacat
     3001 cggccagctc ccggcacgtc ttcactgcat cgtcgagcag ccatccgccg ttggggtcca
     3061 acgtgatccg ggcttccggg aagcggtcgg cgagcgcgat gaccgccttg gcctcatcgg
     3121 gtgcgggcag cacgccgccc ttgagcttga agtcggcgaa cccgtatcgc gccttcgccg
     3181 cctccgcgag gcggacgatg gcctcgggcg acagggcctc ctcatggcgg acgcgcagcc
     3241 actcgtcggc cggatcgtcc gaggcgtctt cgtcagcggg cgagcggtac gccagatcgg
     3301 tcttgttgcg gtcgccgacg aagaagaggt agccgagcgc ctgtacccgg ttgcgctgct
     3361 tgccgtcgcc gagcagcgca gcgaccggca caccgagatg ccggccgagc aggtcgagca
     3421 gcgccgattc gacggcggtc acggcatgca cggcgacgcg caggtcgaac gtctgcgcgc
     3481 cgcgtccccc ggcgtcgcgg ccgccgaacg cgcggcgcat gtcgttgagc acggcgtggt
     3541 ggtcgccgat gctgcggcct gtgacgatcg agcgcgcatc ctccagcgtg cgccggatcg
     3601 gctcgccgcc cggcacctca ccgacgccgg tgttgccgtc ggagtcggtg atgatcacca
     3661 ggttccgggt gaagaacggg ccatgcgcgc cgctgaggtt cagcaggatg ctgtcgtggc
     3721 cggcgatcgg gatcaccgtc atctcgcgga cgactggaac ggtcattgcc ttctcctgtt
     3781 actcaagatg cgaaccattt gtcgccgttg gcgagtggcc gtactacgcg cgacaccttg
     3841 tcccccatgg tgcgcagcgc gtcggcgatc tcgcgctcgc gctgcaggtt cagccgctcg
     3901 acaggcaccg acgcactgat cgcgtcctgg gcggggctgc agtaacgcag ggcgacggcg
     3961 aaacacctta gaccagtggt gttctcttgg tcgtcggtgg cgtacccgcg cacccgcact
     4021 tcctcgagcg cctcgttgaa gacgtcccgg tcgacgatcg tctcggtggt caacggcgtc
     4081 aacgcggcgg ggaggtgcgc atcgcgctcg actccgaacc gctccgcgag cagcgacttg
     4141 cccagcgcgg tcgcgtaggc gggcagccgg cgaccgaccc ggctttgaat cctgttgtac
     4201 tgcgtggatt cccgcgtggc gaggtagacg atgtcggtgc cgtcgaggcg accgaggtga
     4261 aaagtctcgt cgagatcctg acggagatcg tcgaggaacg gcgtgatgag cggtaggtac
     4321 gggtcgctgt cgagatagct cgtgccgacg agcagcgccc gaatgccgat gccgtacaag
     4381 gttcccgacg catccgatcg cacccagccc tgcgcgacga gtgtgcgtag cagggcgtgg
     4441 gcgctgctgc gcggcatgtc gagcgcgtcg ctgatctcgc gcagtcgcgc cgggttgtcg
     4501 tgacgggcgg cgagatactc gagcagctcg accgtccgca cggcggactt caccttccgg
     4561 accgcgagat ccggagccgt tgagccggcg acatcgggca ggggttcgtc ggtcacgtca
     4621 ccctcctatc gccagtagtt cgcggccgat cagcacgacc gcgccgaccg ttgagacgcc
     4681 gaccgcaagc caccgcagtc gttgcggatc gagaaatctg ttcatcgggc gcgacagcag
     4741 gtatcccgcg atggcggccg ggatgagcac ggcgaagcct ctcatggtgc gctcgtcgat
     4801 ggacccggtc acggccagcg ccgcgatcga catgatggag ccgacgagga agaatccgct
     4861 catcgtgccg cgcagccgcg cgccggtatt gcgttgccac acaagcgcca tcggcggtcc
     4921 tccgatggcc gtcgccgtgc cgagcacgcc cgacgccgcg cctgccagca ccacgttgcg
     4981 gcgccgcgga gcggggatcc agccgaagct ggtgagcacg acgccgctga gcaccaccgc
     5041 ggccagcagg atggccagcg cccgatgcgg cagcatcgcc agcagcacgg cgcccgcgat
     5101 cgtgccgggt atgcggccga ccaacgccca cccggtgcct cgcagatcga tgtcctcccg
     5161 ttcgcggatc accaccatca gcgtgagcag cgtggcgacc atgatcagcg tcccggggat
     5221 cagcctgggg tcgacgagcg cgacgatcgg cgcggcgagc aaaccgatac cgaagccgat
     5281 cgacgcctgc agcgccgacg cgagcatgac cgtccccgca acaatgcaat accccatggg
     5341 actcacgctg acgccagcac actcccctcc tccaggaggg gatggtggca ttcggcggtg
     5401 tgtccgacta cctccagatc gagcgcgctc ggcggcgcgg tggtcgcgca gacctcggtg
     5461 gccttccagc agcgggtccg gaaccggcaa cccgacggcg ggtttagcgg cgaaggcacc
     5521 tcaccctcga gcaggatgcg ctcgcggcgc tgt
//
LOCUS       DASYLX010001164         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_319363,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001164 DASYLX010000000
VERSION     DASYLX010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319363"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGP27_27550"
     CDS             complement(<1..201)
                     /locus_tag="VGP27_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855086.1"
                     /translation="MSETTSTPEPETAPVTTAAPASQSTYVEREPRRSRLSAVAVWVG
                     IVAGVVFIVAVIFFSGFILGAHA"
     gene            complement(278..1396)
                     /locus_tag="VGP27_27555"
     CDS             complement(278..1396)
                     /locus_tag="VGP27_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEV7855087.1"
                     /translation="MLAITSISALAAPAAHADPPSPIPEPILPSLAPGQVLRIGPTAG
                     TGTPTGDYGVGATDLCEFMEFPSGILQVCGDSFAGQGVGFGPWHAPIALHVETDSIDD
                     PTGVRYDGVTGTDKPLLADRAKPGESQLPAGVVQINRDNYMMVSTAEDLRPKSSRLVK
                     ADPAQGNWPTLPGSQRDAGFEGGRQSQISGYYDPIPAADSPTGWVYIVANNFDRTGPA
                     VLYRAKRETFADRSSWQGWSSDGGWGRRPTPLWPDRVGEMSIRQIDGKTVLSYFNASN
                     GNMEVRVAENPTALGTAPVTTVVFAGEWPDPAEDLPPPEVNRLAQPYGGYISPGSTLD
                     ELRVFISQWNTLPRAQAPYRVIQFAVNPLKPGEPLPLG"
BASE COUNT          239 a          478 c          509 g          274 t
ORIGIN      
        1 cgcgtgtgca ccgaggatga agccgctgaa gaagatgacc gcgacgatga agacgacgcc
       61 ggcgacgatg ccgacccaga cggctaccgc gctgagcctc gagcggcgcg gctcgcgctc
      121 gacgtaggtc gattgcgacg ccggcgcagc tgttgtcacg ggcgccgttt ccggttcagg
      181 agtggacgtt gtttcgctca tgccttcgag catgcaaacg cttgtgatgc gcctgtcttg
      241 atttacttat gaatcagctg tgaatgagga gttcgcgtca gccgagcggg agtggctctc
      301 ccggcttgag cggattcact gcgaactgaa tgacgcggta gggcgcctgc gcacgcggca
      361 gggtgttcca ctgactgatg aatacccgca gctcgtcgag cgtcgaaccc ggcgagatgt
      421 agccgccgta gggctgcgcc aatcggttca cctcgggtgg gggcagatcc tcggcaggat
      481 cgggccactc gccggcgaac acgacggtgg tgaccggcgc ggtgcccagc gcggtggggt
      541 tctcggcgac ccgcacctcc atgttgccgt tactggcgtt gaagtaggac agcaccgtct
      601 tgccgtcgat ctgccgaatg ctcatctcgc cgaccctgtc cggccacagt ggtgtgggcc
      661 gcctgcccca gccgccgtcc gaggaccaac cctgccagct ggaccggtcg gcgaaggtct
      721 cccgcttcgc gcggtacaga accgctggtc cagtgcggtc gaagttgttg gcgacgatgt
      781 acacccagcc ggtaggcgag tcggccgccg gaatggggtc gtagtagccg ctgatctggc
      841 tctgcctgcc gccctcgaaa cctgcgtcgc gttgcgagcc gggtagcgtg ggccaattcc
      901 cttgcgccgg atccgctttc accaaccggg atgacttggg ccgcagatct tcggcggtgc
      961 tcaccatcat gtagttgtcg cggttgatct gcacgacgcc cgcgggcaac tgcgactccc
     1021 ccggcttcgc gcggtctgcg agcagcggct tgtcggtgcc ggtgacgccg tcgtatcgca
     1081 cgccggtggg gtcgtcgatc gagtcggtct cgacgtgcag cgcgatgggt gcatgccacg
     1141 gtccgaagcc gacgccctgt cctgcgaagc tgtcaccgca cacttgcaag atgccgctgg
     1201 ggaactccat gaactcgcac aagtcggtgg cgccgacgcc gtaatcgcct gtcggcgtcc
     1261 cggttccggc cgtcggcccg atgcgcagca cctgacccgg ggccagcgac ggcaggatcg
     1321 gctccggaat cggcgacggc ggatcggcat gtgctgcggg cgccgcaagc gccgaaatcg
     1381 acgttatggc gagaattact cgaacatttc cgcccaaacg tgagtttcgg cgagagcggc
     1441 tcacaactcg gcggccagca gctcggctat ctgaatggtg ttcagcgcgg cgccttttcg
//
LOCUS       DASYLX010001165         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1743254,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001165 DASYLX010000000
VERSION     DASYLX010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743254"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..786)
                     /locus_tag="VGP27_27560"
     CDS             complement(46..786)
                     /locus_tag="VGP27_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7855088.1"
                     /translation="MEPVFRALELTCSAGVAVSGTRITYRGLENIPARGGAVIAVNHT
                     SYLDWMPAALAAYKRHRRLRFMIKQEMTEVKAVNFLIRHTGTIPVDRRAGAGAYEVAV
                     QRLRAGELVAVYPEATISRSFELKEFKTGAARMAIDAQVPIVPLIVWGAHRRWTKDHP
                     RKLGRAKIPVTVMAGAPVQAADTIELTDAAMREAMTTALHQVQESYEHPAGAYWVPRR
                     MGGSAPTPEEAKVLDEIELAERARKQPR"
     gene            complement(792..1568)
                     /locus_tag="VGP27_27565"
     CDS             complement(792..1568)
                     /locus_tag="VGP27_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7855089.1"
                     /translation="MEPVYGTVIQLARLMWKAQGLKFTVTGVENLPVTGGAVVAINHT
                     GYFDFTFAGLPAYRQKRGRKVRFMAKKEVFDHKITGPIMRSLRHIEVDRTSGGESFEQ
                     ACQKLKEGELVGVYPEATISRSFEIKECKSGAARMAIACDVPIVPHIVWGAQRVWTKG
                     HPKNMWRPKVPITVAVGEPIMPTLPATELTALLHSRMQHLLEKVQDEYGPHPPGEYWV
                     PHRLGGGAPSLAEANLMDADEAAEKAARRAQRGDPAGASG"
     gene            1714..>2913
                     /locus_tag="VGP27_27570"
     CDS             1714..>2913
                     /locus_tag="VGP27_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7855090.1"
                     /translation="MQVTSVGHAGFRIDTQAGSILCDPWVNPAYFGSWFPFPDNSALD
                     WDALGNCDYLYVSHLHRDHFDPDHLRAHVNKDAVVLLPDYPVPDLQRELEKLGFHRFF
                     ETTDSVKHTVSGPKGDLDVMIIALRAPADGPIGDSGLVVDDGETVAFNMNDARPVDLD
                     VVHADFGQVDVHMLQYSGAIWYPMVYDMPARAKEAFGTQKRQRQMDRCRQYISQVGAT
                     WVVPSAGPPCFLDPELRDLNDDRGDPANIFPDQMVFLDQMRTHGHHGGLLLIPGSTAD
                     FTGTQLNSVTHPVPDDEVEAIFTTGKADYIADYADRMAPVLAAEKARWAPAEGESLLE
                     PLRARFEPIMSQSDQICDGIGYPVELRLGAETVVLDFPKRAVREPIPDEKFRYGFAIA
                     PELVRTVL"
BASE COUNT          450 a          997 c          939 g          527 t
ORIGIN      
        1 gcaggcaagg tcatcgcggc gatccacctt cgtttctcgc ctcggtcatc gcggctgctt
       61 acgcgcgcgc tcagccagct cgatctcgtc gagcaccttg gcctcctcgg gcgtcggagc
      121 cgagccaccc atccgccggg gcacccagta cgcgcccgcc ggatgctcgt aggactcctg
      181 aacctgatgc aacgccgtgg tcatcgcctc ccgcatcgcg gcgtcggtca gctcgatggt
      241 gtccgcagcc tgcaccggcg ccccggccat cacggtgacc gggatcttcg cgcgaccaag
      301 cttgcgcgga tggtccttgg tccaccggcg atgggcaccc caaacgatca gcggaacgat
      361 cggcacctgc gcgtctatcg ccatccgggc tgcgcccgtc ttgaactcct tgagctcgaa
      421 gctgcggctg atggtggcct ccgggtacac ggcgacgagt tcgcccgcgc gcagccgttg
      481 caccgccacc tcgtacgcgc ctgcgcccgc tctgcggtcg accgggatcg tgccggtgtg
      541 tcggatcagg aaattgacgg ccttcacctc cgtcatctcc tgcttgatca tgaagcgcaa
      601 ccggcggtgc cgtttgtagg ccgccagggc ggcgggcatc cagtccaggt agctggtgtg
      661 gttgaccgcg atcaccgcac ctccgcgtgc agggatgttc tccaggcccc ggtaggtgat
      721 tcgggtgccc gatacggcga cgcctgccga gcaggtgagc tccagcgcgc ggaaaaccgg
      781 ttccatcgtc tctagcccga cgctcctgcc gggtcacccc gctgcgcacg ccgggcggcc
      841 ttctcggcgg cttcgtcggc gtccatcaga ttcgcctcgg ccagcgacgg cgccccgccg
      901 ccgagccggt gcggcaccca gtattcgccc ggcggatgcg ggccgtactc gtcctggacc
      961 ttctccagca gatgctgcat ccgcgagtgc agtagcgcgg tcagctcggt cgcgggcagg
     1021 gtcggcatga tcggttcgcc gacggccacg gtgatgggca ccttgggccg ccacatgttc
     1081 ttcggatgcc ccttcgtcca cactcgctgc gcaccccaga cgatgtgggg cacgatcggc
     1141 acatcgcagg cgatggccat ccgcgccgcg cccgacttgc actccttgat ctcgaagctg
     1201 cggctgatgg tggcttcggg gtagacgccg accagttcgc cttccttgag tttctggcag
     1261 gcctgctcga aggactcccc gccgctggtg cggtccacct cgatgtggcg caggctacgc
     1321 atgatcggcc cggtgatctt gtggtcgaag acttctttct tggccatgaa ccgcaccttg
     1381 cggccgcgct tctgccggta ggcgggcagc ccggcgaagg tgaagtcgaa gtagccggtg
     1441 tggttgatcg cgacgacggc accgccggtg accggcaggt tctccactcc ggtgaccgtg
     1501 aacttcagcc cctgcgcttt ccacatcagg cgggcgagct ggatgacagt cccgtatacc
     1561 ggttccacat caggcagcct agtcgcgtgt ctgcgccccg cgaacccggt cgtcgccggc
     1621 cgggttgcct tccaccgacg gcggtggaac gccggcgacg tctcgtcgca ttagaatcgg
     1681 gcggacggac atttccccag cgaaagggca ttggtgcagg tcacaagcgt cgggcacgcc
     1741 ggtttccgga tcgacacgca ggccgggagc atcctgtgcg acccctgggt gaacccggcc
     1801 tacttcggtt catggttccc gttccccgac aacagcgcat tggactggga cgcgctcggc
     1861 aactgtgact atctctatgt ctcgcacctg cacagggacc acttcgatcc cgaccacctg
     1921 cgtgcgcacg tcaacaagga cgcggtcgtg ctgctgcccg actatccggt gcccgatctg
     1981 cagcgcgagc tggagaagct gggcttccat cgcttcttcg agacgaccga ctccgtcaag
     2041 cacaccgtca gtgggccgaa gggcgatctc gatgtgatga tcatcgcgtt gcgcgctccc
     2101 gcggacggtc ccatcggtga ctccggtctg gtggtcgacg acggagaaac ggtggcgttc
     2161 aacatgaatg acgccaggcc ggtcgatctc gacgtggtgc atgcggactt cggtcaggtc
     2221 gacgtgcaca tgctgcagta ctcgggcgcg atctggtatc cgatggtcta cgacatgccc
     2281 gcgcgggcca aggaggcgtt cggcacccag aagcggcagc gccagatgga ccgttgtcgt
     2341 cagtacatca gtcaggtcgg cgcgacctgg gtggtcccgt ccgccggtcc gccgtgcttt
     2401 ctcgatccgg aactgcgcga ccttaacgac gaccgcggcg acccggccaa catcttcccc
     2461 gaccagatgg tgttcctcga ccagatgcgc acccacggcc accacggcgg tctgctgctg
     2521 atccccggtt cgacggccga tttcaccggc acccagttga attcggtgac acatccggtg
     2581 cccgatgacg aggtggaggc gatcttcacc accggcaagg cggactacat cgccgactac
     2641 gccgatcgga tggcgccggt gctcgcggcc gagaaggcgc gctgggcgcc cgcagaaggc
     2701 gagtcgctgc tggagccgct gcgcgcacga ttcgagccca tcatgagcca gagcgaccag
     2761 atctgcgacg gcatcggcta tcccgtcgaa ttgcgcctgg gtgccgagac ggtggtgctg
     2821 gacttcccga aacgtgctgt gcgcgagccc attccggacg agaaattccg ttacgggttc
     2881 gccatcgcac cggaactcgt gcggacggtg ctg
//
LOCUS       DASYLX010001166         9479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_536947,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001166 DASYLX010000000
VERSION     DASYLX010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536947"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="ygiD"
                     /locus_tag="VGP27_27575"
     CDS             complement(<1..544)
                     /gene="ygiD"
                     /locus_tag="VGP27_27575"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEV7855091.1"
                     /translation="MPAAFVGHGSPMNAIEVNRYTSAWKAFGQQVKRPRAILVISAHW
                     YINATAVTAMSRPRTIHDFYGFPPELFDVQYPAAGLPELADEVSDVVHPTWVGADLDS
                     WGIDHGTWSVLVHAFPDASIPVVQLSINADKPLGYHLDLGAKLAPLRERGVLVIASGN
                     VVHNLRAMDWSLGDSGYDWAK"
     gene            826..2091
                     /locus_tag="VGP27_27580"
     CDS             826..2091
                     /locus_tag="VGP27_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7855092.1"
                     /translation="MSSTSNPPAAVLGGLSACRSWQEDLYRDIHQHPELSHQEHRTAK
                     LVAEMLRGSGYEVREGIGGTGVVGVMRNGSGPTVLMRADMDALPVEEATGLSYASSVR
                     ATDTAGSEVPVMHACGHDVHVTCLLGAAALLAEGTGNWTGTAIALFQPAEEVGDGARG
                     MVQDGLAEAIPPVDVALAQHVLPAPAGHVGTHRGAVLSAADSMRITVYGRGAHGSMPQ
                     AAVDPVVLAAMIVVRLQTIVSREVAPIDTVVLTIGSIHAGSKSNVIGDRAVLELNLRT
                     YDESVRTAVFDGIRRIVTAECEASNSPKQPDFELYDRFPLTVNDDDVTDRVSDAFAGY
                     FGERYTTMAAQSASEDFSDIPNALGVPYTYWGIGGIDEQQYRKAAAAGRVAQEIPVNH
                     SPTFAPVIQPTLDTGTEALVVAALAWLAG"
     gene            complement(2093..2776)
                     /locus_tag="VGP27_27585"
     CDS             complement(2093..2776)
                     /locus_tag="VGP27_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEV7855093.1"
                     /translation="MITEPPLLLVLDLTGTFAFGLNGALTAVRTARLDVVGVVTLGMI
                     TALGGGVIRDVCIGSVPPATFRDWRYFALAIGGALIAFALSKWLARLETPITVFDAVG
                     LSVFAVIGTSKAVEFNLGIGPAVLLGVVTAVGGGTIRDTLLGQVPTVLRSELYAIPAL
                     IAAAITVITIHLNVYGLPAASAAAAACFIIRMLGVRFGLNAPRPPGAGQDSGSPNCDP
                     PASGPADRQ"
     gene            complement(2808..3569)
                     /locus_tag="VGP27_27590"
     CDS             complement(2808..3569)
                     /locus_tag="VGP27_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEV7855094.1"
                     /translation="MTSDRPWIGWAAVAVGVCAVVASFVASSTRVGEGFAFGFGAFIA
                     FFGVLAVLARNRTPDHWGLVVVGLAMFIVPFLGNGYKADPGASWTCWVAGGLAMILGG
                     IGWVGDKTPTEYGINEIGSGQAQRSALSFWIGRAALVVGLASVLLAIAARTTAAGVAV
                     TIGLGGLTAVIAVWSLLAVDPTHDFLTLAVTGFALFLSPWVAGFSGDTAAWTAWIAGA
                     LLTALGVAGYLRGEQLDFATTVRDDAATRYRRRFR"
     gene            3616..4011
                     /locus_tag="VGP27_27595"
     CDS             3616..4011
                     /locus_tag="VGP27_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV7855095.1"
                     /translation="MARASRDQRSLAADPARRLRRLGGRHFRDPEFDELGDAEKVKYC
                     VTEVAATLGRLDDMIARLAALRTEVDDPDGLVRFTLGDDGRLLTLYIDDAVALPLTEL
                     ALEHKLNDMTSAGNEAMRLSRKEFWDSTG"
     gene            complement(4673..5002)
                     /locus_tag="VGP27_27600"
     CDS             complement(4673..5002)
                     /locus_tag="VGP27_27600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855096.1"
                     /translation="MSQWDPGAEEPEAPSAAVLVPVRLDPLSLPIEGLMVETGVIDAS
                     LVPRPLVPTMLIWSPLIAVALIGPPLIPLPLIGSSPIATVSPLVALAVLHPALTRVRR
                     RRTQSDS"
     gene            5588..5752
                     /locus_tag="VGP27_27605"
     CDS             5588..5752
                     /locus_tag="VGP27_27605"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEV7855097.1"
                     /translation="MTADNAVAQALRQNDIDVISVQIGLLYEQPRLFYLHYWATGDTL
                     SLRAALDRLR"
     gene            5838..6329
                     /locus_tag="VGP27_27610"
     CDS             5838..6329
                     /locus_tag="VGP27_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEV7855098.1"
                     /translation="MTVFSGLPAHVLLVHFMVVLVPLTALLEIVCAVWPAVRRGQIVW
                     LTLILAIVTTVLTPITTNAGEWLYDLRRNPDPVLRVHAERGDWMIYFSVALLVVAIAL
                     AVLQVAERRSGKHRLAASVIVAVLAVAVGISSVVQLYRIGDSGAQSVWGNEIAHLKQA
                     TGK"
     gene            complement(6504..6647)
                     /locus_tag="VGP27_27615"
     CDS             complement(6504..6647)
                     /locus_tag="VGP27_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855099.1"
                     /translation="MDVRSGFEAMGESPAKVAVTVTEFAVIQSEELVADLLLEWFVAH
                     PSI"
     gene            7014..8258
                     /locus_tag="VGP27_27620"
     CDS             7014..8258
                     /locus_tag="VGP27_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV7855100.1"
                     /translation="MVLIAAVGLYVAEFVRWPAYVTQIDLMVYRFGGHRVLQGLDLYS
                     TGIFGSHRVLLFTYTPFAALCFTPLNMLSLPSLRALSLAAGVALLTYSVQRMLVALDT
                     PRRPRWWTLTAMLVGLCLWLEPVRYSIQLGQINLVILAVVVAELLGSPERKWAGVGIG
                     LVAGIKLTPAIFLIYLVLIGQRRAGLVGTVAFLVTVALGFAILPGDSATYWLGGKFDN
                     PQRVSPDPTVSSSVRGLFLRLHYPTGVATAVALALGLLCLAVAVIAWCRGHRVLGLSL
                     VGQASAAASPFSWSHHWVWLVPLTVHLGYRGIVLGSRRAVWAMWLLGALTAGWLVFPQ
                     GKAPISGLVAWRPGGLWNTLLPSGYLLVMLVAVIASAVWLRSTASRSRAKPVAVSSTG
                     PPADAGNSVPAVIGGDRWPLDP"
     gene            8493..8834
                     /locus_tag="VGP27_27625"
     CDS             8493..8834
                     /locus_tag="VGP27_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855101.1"
                     /translation="MKSDNNTTTVWRRITATALGAGAIAVGATVLGAPAAQASPANIP
                     AAQFKQNCLSTSVPSTQRVEYKVFRVDSDLHQVCTIYSGNYALQSDANVVVGYFTQTI
                     KPGSDRVSSVA"
     gene            9367..>9479
                     /locus_tag="VGP27_27630"
     CDS             9367..>9479
                     /locus_tag="VGP27_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HEV7855102.1"
                     /translation="MLRVTLRDLQWRRRRFVITIVGTSLVFAMTLLLTGLS"
BASE COUNT         1736 a         2953 c         3025 g         1765 t
ORIGIN      
        1 gtttggccca gtcataaccg ctgtcaccga ggctccagtc catcgcgcgc agattgtgca
       61 cgacattgcc gctggcgatc accagcacac cgcgctctcg caatggagcc agcttggcgc
      121 ccaagtcgag gtggtagccc aacggcttat cggcattgat cgacagctga acaaccggaa
      181 tcgacgcgtc ggggaatgca tgcaccaaca ccgaccacgt cccgtgatcg ataccccagc
      241 tatcaagatc tgcgcccacc cacgtcggat gaaccacgtc agagacttca tcggcgagct
      301 cgggtaaccc agcggctggg tactgcacgt cgaacaactc cggcggaaac ccatagaagt
      361 cgtggatcgt gcggggacgc gacattgcag tgacagccgt ggcgttgata taccagtgcg
      421 cgcttatcac caagatcgcg cgagggcgct tgacttgttg cccgaaggct ttccacgccg
      481 atgtgtatcg gtttacttca atcgcattca tcgggctgcc atgtccgacg aaggccgccg
      541 gcattgtctg aactcgaccg gcggcggcag tcacccgcaa ctcctgcagc gaatcatctg
      601 ggtccacctc tatctacttt ctactcttgc ctgcaaaggc cgacgatcac gtcgttgcca
      661 tgtttagagg caagtccacg acctcccaag tacatcgcga cttgtgggta tgcatcgccc
      721 aacgggcagc ccggaaagtg atcgccacct atcttggggg tatatgcggt cgcgccaacg
      781 gatcccgtgc cggcattgtc ggtcatcgaa gcgatcggag gaaagatgag ttccacatcg
      841 aacccgccgg ccgcggtcct cggcggcctt tcggcctgcc gatcgtggca agaagatctc
      901 taccgtgaca tccatcagca tcctgaactg tcgcatcaag aacatcgaac cgcgaaactg
      961 gtagccgaaa tgctgcgtgg ctccggctac gaggtgcgcg aaggaatcgg gggcaccggc
     1021 gtggtcgggg tcatgcgaaa tggcagcgga ccgacggtgt tgatgcgcgc cgacatggac
     1081 gcgctgccgg tcgaggaggc caccggcctt tcctatgcca gctcggtgcg cgcaaccgac
     1141 accgcaggta gcgaggtgcc tgtgatgcat gcctgcggcc atgacgtaca cgtcacctgt
     1201 ctgctcggcg ccgctgcgct actcgccgaa ggaaccggca actggacggg aaccgcgatc
     1261 gcgctgtttc agccggccga agaagtcggt gacggtgccc ggggaatggt ccaggacggg
     1321 ctggccgagg cgattccgcc ggtcgacgtt gcccttgccc agcatgtgct tccggcgccg
     1381 gcaggccacg tcggcaccca tcgtggcgct gtcctctcgg cggccgacag tatgcgaatc
     1441 acggtctacg gccgcggcgc gcatggttcg atgccgcagg ccgctgtcga cccggtggta
     1501 ctggcggcga tgatcgtggt ccggctgcaa acgatcgtct cgcgcgaggt tgctcccatc
     1561 gacaccgtcg tgctgacgat aggaagcatc catgccggca gcaagagcaa tgtgatcggt
     1621 gaccgcgctg tgctggagct gaacctgcgc acctatgacg aatccgtacg tacggccgta
     1681 tttgatggga tccggcgcat cgtcaccgcc gagtgtgagg cgtcaaactc accaaagcaa
     1741 cccgatttcg agctttacga ccggttcccc ctgaccgtga acgacgacga tgtcacggac
     1801 cgggtgagcg acgcgttcgc gggctacttc ggcgagcgct acacaacgat ggcggcgcag
     1861 tcagccagtg aggacttcag cgacatcccg aatgcgctcg gcgtgcccta cacctattgg
     1921 ggcatcggcg gaatcgatga acagcaatac cgcaaggcgg cggctgcggg gcgggtcgcg
     1981 caggaaatcc cggtcaatca ctcgccgacg ttcgctccgg tcattcaacc caccctcgat
     2041 accggcaccg aggcactcgt cgttgccgcg ctggcctggc tcgccggctg attcactgcc
     2101 gatcggcagg ccccgacgcg ggaggatcgc aattcggaga cccggaatct tggcccgcac
     2161 cgggcgggcg gggagcgttc aggccgaacc ggactccgag catgcggatg atgaagcagg
     2221 ccgccgcggc cgctgaggcg gccggtaatc cgtacacgtt caagtggata gtgatgacgg
     2281 taatcgcggc agctatcagg gcagggatcg catacagttc gctgcgcagc accgtgggga
     2341 cctgccccaa tagggtgtcg cggatggtgc ctccgcccac cgcggtgacg acaccaagta
     2401 ggaccgcggg cccgattccg agattgaact ccactgcctt gctcgtgccg atgaccgcga
     2461 acacgctcag cccgaccgcg tcgaataccg tgatcggagt ctccagccgg gccagccact
     2521 tgctgagcgc aaaagcgatc agtgcgccac cgatggcgag tgcgaagtag cgccagtcac
     2581 gaaaggtggc agggggtacg gaaccgatgc aaacgtcgcg gatgacccca ccgcccaacg
     2641 ccgtgatcat gccgagggtc acgacgccca ccacgtcgag ccgcgcagtg cgcaccgctg
     2701 tcaacgcccc gttgagtccg aaggcgaatg tgccggtcag atccagcaca agcaacagcg
     2761 gcggttcggt gatcacactc tccttccgcg tgcacgccgg gaagtggtca gcggaatcgt
     2821 ctgcggtacc gggttgcggc atcgtcacgg acggtggttg cgaagtccag ctgctcaccg
     2881 cgcaggtatc cggccacgcc gagcgcagtg aggagggcac ccgcaatcca ggccgtccac
     2941 gcggcggtgt ccccggagaa cccggcaacc catggcgaca ggaatagcgc gaatccggtg
     3001 acagccagcg tcaggaagtc gtgggtggga tcgaccgcga gcagcgacca gacggcgatc
     3061 accgccgtca gccctcccag cccaatcgtg acggcgaccc cggctgctgt cgtgcgtgcc
     3121 gcgatagcca gcaggacgct ggcgagaccg acgacgagcg ccgcgcgccc gatccagaaa
     3181 ctcagggcgc ttctctgcgc ttgcccactg ccgatttcat tgatgccgta ctcggttggc
     3241 gtcttgtcac ccacccagcc gatgccgccg aggatcatcg cgagcccccc cgccacccag
     3301 cacgtccatg aggccccagg gtcggcctta tagccgttcc cgaggaacgg cacgatgaac
     3361 atggccagcc ccacgacgac gagcccccag tgatcgggtg tgcggttgcg tgccaagacg
     3421 gccagcaccc cgaagaaggc gatgaacgcg ccgaagccga acgcgaagcc ttccccgact
     3481 cgcgtcgagc tggccacgaa cgacgccacg acggcgcaga cgccgaccgc tactgcagcc
     3541 caaccgatcc atggtctatc agaagtcaca gaaccaccta acacccatgc acccatacct
     3601 gtgtggcgta tctgcttggc tcgggcaagt cgggatcagc gctcgctggc tgctgatccg
     3661 gcccgacggc tacgtcgctt gggcggcaga cactttcgag atccggaatt cgacgaactc
     3721 ggggacgccg agaaggtgaa gtactgcgtg acggaagtcg ccgcaacgct cggccggctc
     3781 gacgacatga tcgctcggct ggcagccctg cgcaccgagg tggacgaccc tgacggtctg
     3841 gtccgtttca cactgggtga cgacggtcga ctactcacct tgtacatcga tgatgccgtg
     3901 gcattgccac tgacggaact ggccttggag cacaagctca acgacatgac gtctgctggc
     3961 aacgaggcaa tgcgcctgtc gcgcaaagag ttctgggaca gcacgggctg acccgggtcg
     4021 ccgcgcctgc gaggagagga tgtgagctga aggcgtcctc ttccgttaaa ggctgactgc
     4081 gaatcaaatt gtggcgacga ggtgtccctt aggcagcgga atggaggtag gtcatccacg
     4141 tgccatgctg gcgcggacga gctgaccctc gtcgatgctc agcgaacctt tcatcgcccc
     4201 gaggctcaac gggactcgac gctaaaactg accttagtgg gctagtcgca attcgctgat
     4261 cgttgggatg ctggggcggt tcgcgccgtc tttccccgcc accggtgcca gtgccaggcc
     4321 taacgctgtt gccggcgccg cccagctcct acagcatgcg cacccacagc tcggcgctat
     4381 tcgaagctgt gtgttcacct agctgggcta gcagtttgct gtgaaggtcc cctggggacg
     4441 ggtcatcatg tttcgcgtgc ccacgctgcg gttacccagt cggcgtcagg gggtcgcgac
     4501 ttgccttcgc cgtgtatacg acggcgatgt gccgggtctt atatcgcagg tcagcgtgcg
     4561 tgtatgccac gttgctggcg gacagacgag aaacgagagg ttcatgatgg atctgaaaaa
     4621 aatggcggcg ggagccgtga tcgctggggc tctcgggctg cccacgcttt cgctaggagt
     4681 cggattgggt acggcgtctg cggaccctgg tcagtgctgg atgcaaaact gccagggcga
     4741 ccagcgggga gacggtggcg atcggagagg acccgatcag tggcaagggg atcagtggcg
     4801 gcccgatcag cgcgacggcg atcagtgggg accagatcag catggttggg accaacggcc
     4861 gtgggaccaa cgaggcgtcg atgacgcccg tttcgaccat cagcccttca attggcaagg
     4921 acagcgggtc gagccgtact gggaccaaga ccgcggcgct tggggcttct ggttcctcgg
     4981 cgcctggatc ccactgtgac atcagcaggg tgacggttcc cgccgtgcac gcggcgtgtt
     5041 tcagctggtg gggcgtcggc ggcaaatgtc cgccagcctc accagcgtgg cagacagaca
     5101 caggcggcgg agcgaggggt cgggcgcggg ccttcatggt gaccgggtac tgaggatgtc
     5161 acgccacgag caccatgacg ggtggggctg ggtaggtcat accgtgatga cgcctcaacg
     5221 ctcagtttcg ccctagcgca tccgaaacgc ccgggtagaa ggcatgggcc ggaccgcggt
     5281 tcgtgaccat tttatgggca accgcatgga ttgttcgtgc gcggttcgag cgatttcatt
     5341 cgcctgtcaa accacggcta acaagctgtg tatggacggt gacttgcctg ggcgcggggc
     5401 aatcgccctg gtcgccactg gttgtagctt cggggatccg ggcagccgca gacgcagacg
     5461 ccgcggtcat cagcgccggt gccgtcggtt gactgggccg ctgcggaggg cgcatttggt
     5521 gctcccctgc aggccgaaga tgaggtacac cacgcagata ttccgcgcga cgacttggat
     5581 gtgatggatg accgccgaca acgcggtggc acaagccctg cggcagaacg atattgacgt
     5641 catcagcgtg caaatcggcc tgctctatga acaaccgcgg ctgttctacc tgcactattg
     5701 ggcgacgggg gatacgctca gcctgcgtgc tgcactggac cgcctgcgct agacgacagc
     5761 gacgccgtca cccagccgcg gtagatttct cgcggctgat cctccccgca gctgcggggg
     5821 caaaagagag ggaaggtgtg acggttttca gtggcctacc cgcccacgtc ctgctggtcc
     5881 atttcatggt tgttctggtg ccgctgaccg cattgctgga aattgtttgc gctgtgtggc
     5941 ccgccgtgcg gcggggacag atcgtctggc tgacgctgat tctcgcaatc gtgacgacgg
     6001 tactgacccc catcacgacc aatgccggcg aatggctcta cgacctgcgt cgcaaccccg
     6061 accccgtctt gcgagtgcat gccgagcgcg gcgactggat gatctacttc tccgtcgcgc
     6121 tcctcgttgt cgcaattgcg ctcgcggtgt tgcaggttgc cgaacgccgg tccggcaaac
     6181 accggttagc cgcaagcgtt atcgtcgccg ttctggcggt cgcggtcggg atatcgtcgg
     6241 tcgtgcagct gtaccgtatc ggtgacagcg gcgcccaatc ggtgtggggc aacgagatag
     6301 cacacctgaa acaggccacc ggaaagtaga gtcgccggtt caccttgaga atgtgccacc
     6361 atctgaccgg ggtcggccac tcgcatgaca gcaattcgac gacctaatct ggcgcaccga
     6421 tgccgctggc gtgctggtgc tatccgcgag ggcgaatgtc ggttcgactt caccggcgat
     6481 aaatggccga tgtcccggct gactcagatg gaagggtggg caacgaacca ctcgaggagc
     6541 agatcggcga ccaattcctc agactggatg acggcgaatt ccgtaaccgt caccgcaacc
     6601 ttcgccggcg attcgcccat cgcttcaaaa cccgaccgca catccatgcg agcagacaga
     6661 aggcggcatc cgatccgccg ccagcgccga tgtagcgcca gacggctgca cccgcttcga
     6721 tgtcgagatg agaagtgctc ccgtcccgat gaccgggatg aaatcgaata ggcgccttcc
     6781 cgcgcgtgca tcaaagctgc tggtcgaagt gggacgtcgc catcatcgat gaccgtcgcc
     6841 tcgttgcgga ccattcgcgc cgttgtagta ggtggcccga gggtatcgag agggtataga
     6901 gccgtggtga cttgcgccaa ccatggcaac gggacgccgt gacagcggtt tgccgccagc
     6961 aggcgcggcc cgtgtcgtct ggcccgacgc ggtgcctgcg atatggcggg ccggtggtct
     7021 tgatcgccgc cgttgggctg tacgtcgccg aattcgtgcg ctggccggcc tacgtcacgc
     7081 agattgactt gatggtctac cggtttggtg ggcatcgtgt gctgcagggg ctcgacctgt
     7141 actcgaccgg tatcttcggt tctcacaggg tgctgttgtt tacctacacc ccctttgccg
     7201 cgttgtgctt cactccgctg aacatgttga gcctgccgtc gttgcgggcc ttgtcgctgg
     7261 ccgctggcgt cgcattgctg acgtactcgg tgcaacggat gcttgtcgca ttagacacgc
     7321 cgcgccgtcc gcgatggtgg acgctgaccg caatgctggt cggtttgtgc ctctggttgg
     7381 agccggtgcg ctactcgatc cagctcggcc agatcaacct cgtcatcttg gctgtcgtcg
     7441 tcgctgagct gctgggctcg cctgagcgga aatgggccgg cgttggcatc ggtttggtgg
     7501 ccgggatcaa gttgactccg gcgatatttc tgatctatct cgttctgatc ggccagcgcc
     7561 gggccggatt ggttggcact gtcgcctttc tcgtcaccgt ggcgctcggc ttcgcgatcc
     7621 ttccaggtga ctccgcgacg tattggctcg gcgggaagtt cgataacccg cagcgggtct
     7681 ccccggaccc gactgtgagc agcagtgtgc gcgggctgtt tctgcggctg cactacccga
     7741 ctggggtcgc caccgccgtc gcgctggcgc tggggctact gtgccttgcc gtcgcagtaa
     7801 tcgcctggtg ccggggacat cgtgtactgg gactgagcct ggttgggcag gcttctgctg
     7861 cggcgtcgcc gtttagttgg agccatcact gggtgtggct ggttccgctg actgtgcacc
     7921 tgggttatcg cgggattgtc ctcgggtcac ggcgcgcggt gtgggcgatg tggctgctgg
     7981 gcgcgctgac cgcgggctgg ctagtcttcc cgcagggtaa ggcgcccata tccgggctgg
     8041 tcgcctggcg ccctggcgga ctctggaata cgttgctgcc cagcggctat ctgcttgtga
     8101 tgctcgtagc agtaatcgcc tcggcggtct ggctgcggag caccgcatcc cggtctcgtg
     8161 ccaaacctgt cgccgtgtcc tcaacgggtc cgccggccga cgctggtaac tcggtgcctg
     8221 cagtcatcgg cggcgatcgt tggccgctgg atccatgaga cgatcaaaaa tcatacttta
     8281 gctttcgtgc ccgggtgcgc cgtaaacagc agtctcacag caacattcca ggtgcggagc
     8341 ccgtgctgac ccggcggata gaaagccctt tcctgctcca agtggactct ggcttgcagg
     8401 ctattacagc gaagcacggg tgaacgcgac catttctcct agcagttgaa cgcagcccac
     8461 aaggggctgt gacaagccaa ggagagaaca ccatgaaaag cgacaacaac accaccacag
     8521 tctggcggcg gatcacagcg acggccttgg gtgcaggagc cattgccgtt ggggccacgg
     8581 tgctgggcgc acccgctgcc caagcaagcc ccgccaacat cccagcggcc cagttcaaac
     8641 agaattgcct cagcacctca gtgccctcga cccaacgtgt cgagtacaaa gtgtttcgcg
     8701 tggacagcga tcttcatcag gtctgcacta tttacagcgg caattacgcc cttcagtccg
     8761 acgccaatgt cgttgttgga tacttcacgc aaacgatcaa gcccggctcc gatcgggttt
     8821 catcggtggc ctagctagga caagaccacg aatctcggcc cgccacttta gatccccacg
     8881 ggtggcgggc ccccgattgt cacgtctcat tgaaggtctt caatcgggca ggtcatcttc
     8941 acgttgtcgc ccctgactac cacaactgcg tccggcccgg ttgtcgagtg cccggtggac
     9001 agaataaaga acttctcggc aaccggatgt ctgtgtggga tattgaagcg gctcgagcgg
     9061 cggacttgat gcgagccggt tactgtcaac ggagtccacg cgatgcggtc atttggtcca
     9121 agcgccctaa cgggcttgca gcgaggttgc cgttatggcg atccccagcc tccggtacgg
     9181 cccaaggcac aacgaactgc atgtgatgtt ggggccgaag cggctatggt ccaggcgtga
     9241 tccacggtaa tgacagccgg taatccgcag gcgagtcgtc acatacttgt gtttctcgca
     9301 aacaacccgc gaatctatct cgcaaacgac cggcgcaagc tcggcccatg tcggtagcgt
     9361 caccacatgc tgcgtgtgac gctgcgcgac ctccaatggc gacggcggcg cttcgtcatc
     9421 acaattgtgg gcaccagcct ggtgttcgcg atgacgcttc ttctcacagg gttgtctaa
//
LOCUS       DASYLX010001167         4356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2951403,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001167 DASYLX010000000
VERSION     DASYLX010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951403"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="VGP27_27635"
     CDS             complement(<1..1805)
                     /locus_tag="VGP27_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7855103.1"
                     /translation="MSRGTGRRGRKTEQAGPSQERSARARRTRQAAPDNGLRSASFVF
                     RHRAGNAVIFLVLVVAAAQLFNLQVPRAAGLRAEAAGQLKVTDVDKAVRGSIVDRNDD
                     KLAFTIEARALTFQPVKVRKQLSDARQKTPEAPDPEARLRDIAAEVALRLNNKPDAKT
                     VLKKLKSNESFVYLARAVDPAISEAISKKFPEIGSERQDLRQYPGGSLAANIVGGIDW
                     DGHGLLGLEDSLDATLAGADGSVTYDRGSDGVVIPGSYRNRHTAVNGSTVQLTLDDDI
                     QFYVQQQVAQAKNASGAENLSAVVLDAKTGEVLAMSNDNTFDPSQDLGRQENRELGNL
                     AVSSPFEPGSVNKIVTAASVIEYGLSNPDEVLQVPGSIDMGGVTVGDAWQHGVTPYTT
                     TGVFGKSSNVGTLMLAQRIGPERYAEMLDKFGLGQRTNVGLPGESEGLVPPIDQWSGS
                     TFSNLPIGQGLSMTLLQMTGMYQAIANDGVRIPPRIVKATIAPDGSRTEEPRPDGVRV
                     VSPQTAQTVRNMLRAVLQRDPMGTQQGTGSTGAVEGYQLSGKTGTAQKINPACGCYYS
                     DVYWITFAGMVTTDNPRYVIGIMADNPHRTAEGQPG"
     gene            complement(1802..2779)
                     /locus_tag="VGP27_27640"
     CDS             complement(1802..2779)
                     /locus_tag="VGP27_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855104.1"
                     /translation="MKAKRPTAARGTDERRRSQRQPTRAAQRRSPEAPPQRGRPRTAE
                     TAPITRPQIKPVRPKNASQAKARAKARKAKAPKIVRPPLKERLLARISEIDLNPRTLA
                     ARVPFVVLVIGSLGLGLGVTLWLSTDAAERSYQLGSARETNQALLQQKEALERDVLEA
                     QAAPALAESARELGMIPSRDTAHLVQDPAGNWIVVGTPKPAEGVPPPPLNAPLPDDSP
                     APPLQAAAGRPAPPLVADPREMVVRVPERTVPTAPAPAPAPAPDPAAVPLAGPLPDSP
                     HGASGQPPALGPAVQPEGPLPAPQPVAPAAAAPAEQFGPVTATVPGAPA"
     gene            complement(2776..3789)
                     /gene="rsmH"
                     /locus_tag="VGP27_27645"
     CDS             complement(2776..3789)
                     /gene="rsmH"
                     /locus_tag="VGP27_27645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV7855105.1"
                     /translation="MAATSDHGHIPVLLDRCVELLKPALTRRGPDGSGAVLVDATLGA
                     GGHAERFLAELPGLRLIGLDRDPNALWIAGERLAPFADRVLLTRTRFDGIAEVLTESG
                     YWTGASGRPEIDGILFDLGVSSMQLDRTERGFSYATDAPLDMRMDPDAELTAAEVVNT
                     YDEKSLARILREFGEERFAGRIAARIVRRRARRPFARTGEIVELLYEAIPAPARRTGG
                     HPAKRTFQALRVAVNGELDVLRDALPAALAALNAGGRIAVMSYQSLEDRIVKNVFAAA
                     TASRTPPGLPVELPGHEPEFASLTRGAERAGATEIELNPRSASVRLRALEKVAERPEG
                     DES"
     gene            complement(3911..4342)
                     /gene="mraZ"
                     /locus_tag="VGP27_27650"
     CDS             complement(3911..4342)
                     /gene="mraZ"
                     /locus_tag="VGP27_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEV7855106.1"
                     /translation="MFLGTYTPKLDDKGRLTLPAKFRDALAGGLMVTKSQDHSLAVYP
                     RSEFEALARRASGASRSNPEARAFLRNLAAATDEQHPDAQGRITLSADHRRYANLSKD
                     CVVIGSVDYLEIWDSQAWQNYQEIHEENFSAASDETLRDII"
BASE COUNT          602 a         1445 c         1558 g          751 t
ORIGIN      
        1 cccggctgcc cctccgcggt gcggtgcggg ttgtcggcca tgatgccgat cacgtaccgc
       61 gggttgtcgg tggtgaccat gccggcgaag gtgatccagt agacgtcgct gtagtagcag
      121 ccgcacgccg ggttgatctt ctgtgcggta ccggtcttgc cggagagttg atacccctcg
      181 acggctccgg tcgaccccgt gccctgttgc gtgcccatcg ggtcgcgttg cagaaccgcg
      241 cgcagcatgt tgcgcaccgt ctgcgccgtc tgcggcgaaa cgacccgcac gccgtcgggg
      301 cggggttcct cggtgcggct gccgtcgggg gcgatggtgg ccttcacgat gcgcggcggg
      361 atgcggacgc cgtcgttggc gatcgcctgg tacatacccg tcatctgcag cagcgtcatc
      421 gaaaggccct gtccgatggg caggttggag aaggtgctgc cggaccactg gtcgatcggc
      481 ggtaccaggc cctcgctctc gccgggcaga ccgacgttgg ttcgttgacc gaggccgaac
      541 ttgtccagca tctcggcgta gcgctccggg ccgatgcgct gcgccagcat cagcgtgccg
      601 acgttcgagg acttcccgaa cacgccggtg gtggtgtacg gcgtcacgcc gtgctgccac
      661 gcgtcgccga cggtgacgcc gcccatgtcg atggagccgg gcacctgcaa cacctcgtcg
      721 gggttggaca gcccgtactc gatgaccgac gcggcggtga cgatcttgtt caccgagccc
      781 ggctcgaacg gtgacgagac ggccaggttg ccaagctcgc ggttctcctg gcggccgagg
      841 tcctggctgg ggtcgaacgt gttgtcgttc gacatcgcaa gcacttcacc ggttttcgcg
      901 tcgagcacca ccgcggagag gttctccgca ccggaggcgt tcttggcctg cgcaacctgc
      961 tgctggacgt agaactggat gtcgtcgtcg agggtcagct ggacggtgga tccgttgacc
     1021 gcggtgtgcc ggttgcgata gctgcccgga atcaccacgc cgtcggagcc gcggtcgtag
     1081 gtgaccgacc cgtcggcccc ggccagcgtg gcatccagcg agtcctccag cccgagcagc
     1141 ccgtggccgt cccagtcgat gcctccgacg atgttggccg ccagcgaacc gccgggatac
     1201 tggcgcaggt cctggcgttc ggaaccgatc tcggggaact tcttcgagat ggcctcggag
     1261 atcgcgggat cgactgcgcg ggcgaggtat acgaacgact cgttgctctt cagcttcttc
     1321 agcaccgtct tggcatcggg cttgttgttc agccgcagcg ccacctccgc ggcgatgtcg
     1381 cgcagccggg cctcggggtc gggtgcctct ggcgtcttct gcctggcgtc ggacagctgc
     1441 ttgcggacct tgaccggctg aaacgtcagc gcacgcgcct cgatggtgaa cgcgagcttg
     1501 tcgtcgttgc ggtcgacgat gctgccgcgc accgccttgt cgacgtcggt gaccttgagc
     1561 tgacccgccg cttctgcgcg cagccccgcg gcgcgcggca cctgcaggtt gaacagctgc
     1621 gcagcggcga cgacgagcac caggaagatg acggcgttac cggcccggtg ccggaacacg
     1681 aaagacgcac tgcgcagacc gttgtcgggc gccgcctgcc gggtacgacg cgcacgggcc
     1741 gaccgctcct ggctcggacc cgcctgctcg gtcttgcggc cgcgccgacc ggtgccgcgg
     1801 ctcacgccgg ggcgccgggg actgtcgcgg tgaccgggcc gaactgctcg gcgggcgcag
     1861 ccgcggcggg tgcaacgggc tgcggcgcgg gcaggggccc ttcgggctgt acggcaggtc
     1921 caagcgcggg cggctggccg ctcgcgccgt gcgggctgtc gggcagcggc ccggccagcg
     1981 ggacggcggc cggatcaggc gcgggggcgg gcgccggggc gggggctgtg ggcacggtcc
     2041 gttccggcac ccggacgacc atctcgcggg ggtcggcgac cagcggcggt gcgggccggc
     2101 cggcggcggc ctgcaaaggc ggcgcggggc tgtcgtccgg tagcggcgcg ttcaacggtg
     2161 gcggcggaac gccttctgcc ggtttgggtg tgcccaccac gatccagttt ccggccggat
     2221 cctgtacgag gtgtgcggtg tctcgggacg ggatcatgcc cagctcacga gcggactcgg
     2281 cgagggccgg tgccgcctgg gcctccagca cgtcgcgttc gagggcctct ttctgctgca
     2341 gcaacgcctg attggtttcc ctggcactgc ccagctggta tgaccgctca gcggcgtcgg
     2401 tcgacaacca cagcgtgaca cccagcccca ggccgagcga cccgatgacc aggacgacga
     2461 acggaaccct ggccgccagc gtgcgcgggt tgaggtcgat ctccgagatt cgtgcgagca
     2521 accgttcctt caggggcggc cgaacgatct tcggcgcctt ggcttttcgg gccttcgcgc
     2581 gagccttggc ctggctggca ttcttcggcc ggacgggctt tatctgcggg cgggtgatcg
     2641 gtgcggtttc ggctgtgcgc gggcgccccc gttgcggtgg tgcctccggt gagcgccgct
     2701 gtgctgcgcg ggtgggctgg cgctgcgaac gcctgcgctc atcggtgccg cgcgcggcgg
     2761 tgggtcgctt ggccttcatg actcatcccc ttctggcctt tcggcgacct tctccaatgc
     2821 ccgaagccgc acggacgcgc ttcgtgggtt gagttcgatc tctgtcgcgc ccgctcgctc
     2881 ggcgccacgg gtcaggctcg cgaattccgg ttcgtgtccg ggtaattcga cgggcaggcc
     2941 gggtggggtc cgcgacgccg tcgccgcggc gaatacgttc ttgacgatgc gatcctccag
     3001 tgactggtaa gacatcaccg cgatgcgacc accggcattc aatgcggcca gtgcggccgg
     3061 aagcgcgtcg cggagcacgt ccagctcacc gttgacggcg acgcgcagcg cctggaacgt
     3121 gcgcttggcg ggatgcccac cggtgcggcg ggccggtgcc gggatcgcct cgtagagcag
     3181 ctccacgatt tcgccggtgc gggcgaacgg ccgacgggcg cgcctgcgga cgatccgcgc
     3241 cgcgatccgc ccggcgaacc gctcctcacc gaactcacgc aggatgcgcg caagcgactt
     3301 ctcgtcgtaa gtgttgacaa cctcggctgc ggtcagctcc gcgtcggggt ccattcgcat
     3361 gtccagcggc gcgtcggtcg cgtaggagaa cccgcgctct gtgcggtcca gctgcatcga
     3421 cgaaacgccg aggtcgaaca ggatcccgtc gatctcgggt cggccggatg cgcccgtcca
     3481 gtaaccggat tcggtgagca cctccgcgat cccgtcgaat ctcgtccggg tcagcaggac
     3541 gcggtctgcg aacggggcga ggcgctcacc ggcgatccac aaggcgttcg gatcgcgatc
     3601 gagaccgatc aatcgcaggc ccgggagttc ggcgaggaac cgctcggcat gtccacccgc
     3661 acccaacgtg gcgtccacca gcacggcacc ggagccgtcc ggaccacgcc gcgtgagggc
     3721 gggcttgagc agttcgacgc atcggtcgag caggaccggg atgtgtccgt ggtcagaggt
     3781 ggcagccatt gctagccctc cgaggaatgg cgcccctgca ccgtggtctc tgtccgagcg
     3841 agcggacctg gcgtcgggga agtacgccag ggcctgctcg gacagaggcc gcgatacacg
     3901 gtccgccgaa tcaaataatg tcgcgcagag tttcatcgct ggccgcggag aagttctctt
     3961 cgtgaatctc ttggtagttc tgccatgcct gggaatccca gatctcgagg tagtcgaccg
     4021 agccgatcac cacgcagtcc ttggaaaggt tggcgtagcg acgatggtcg gccgacaacg
     4081 tgatccggcc ttgggcgtcg ggatgttgtt catcggttgc ggcggcgagg ttgcgcagga
     4141 acgcccgggc ttccggattg cttcgcgatg cgcccgacgc ccgtctggcc agtgcctcga
     4201 actcggagcg cggataaacg gccaggctgt gatcttggct cttggtgacc atcaaccccc
     4261 ctgccagcgc atcgcggaac ttggcgggca gcgtcagccg ccccttgtcg tcgagcttgg
     4321 gcgtgtaggt gccgagaaac atccccgcca cctccc
//
LOCUS       DASYLX010001168         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2841079,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001168 DASYLX010000000
VERSION     DASYLX010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2841079"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..>1578
                     /locus_tag="VGP27_27655"
     CDS             5..>1578
                     /locus_tag="VGP27_27655"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7855107.1"
                     /translation="MRNPHAGQPFTTPDEQIAEALLDVSIPTLMLSLVHMSGDADLIR
                     GELKPAGLFLNEVQGYMSEEDKAAVRKIALEVIADYRDRGCPEPEPIGADLLHEMMQW
                     LVCEPVPAEYVPMVLEEMELDGRDARAGPTLAKTSPEEFPVVVIGCGESGLLAGIRLK
                     EAGIPFTIIEKNAGVGGTWYQNTYPGARVDVPNHFYCYSFEPTDQWTHFFAEQPELQA
                     YFERVMDKHNIRGHVLWETEVTEACWDDATATWTVRARDRSGRTTELPARAVITAVGQ
                     LDRAHTPTIEGQRDFAGPTFHSSAWDHSVDLHGKRVAMIGAGASGFQIAPTIAPDVEH
                     LNVFQRTAQWMFPNPSYHEKVGQGVQWALRHLPFYGRWYRFLLFWPWCDKGLDVARVD
                     PNYPDQQTAINELSEITRVMFTEWITSQIGDDSDLRAKVVPDYPATGKRTLQDNGSWL
                     KMLTRDNVELVRTEIDHIESGAVVTEDGTRHSADVIVYATGFEATKLLWPMTIRGRGG
                     EVLAERWGERPSAYLG"
BASE COUNT          301 a          500 c          516 g          261 t
ORIGIN      
        1 agtcgtgcgc aacccacatg ccggacaacc ttttacgacc ccggatgagc agattgccga
       61 ggcgctcctc gacgtcagca ttccgacgtt gatgctgtct ctggtgcaca tgtccggcga
      121 cgccgacttg atccgcggag aactcaagcc cgccgggctt ttcctgaacg aggtgcaggg
      181 ctacatgtcc gaggaggaca aggccgctgt tcgcaagatc gccttggagg tgatcgccga
      241 ctaccgcgac cgcggctgtc ccgagcccga accgatcggc gccgacctgc tgcacgagat
      301 gatgcagtgg ctggtgtgcg agccggtgcc agcggagtac gtgccgatgg ttctcgagga
      361 gatggagctc gacggccgcg acgcccgcgc cggtccgacc ttggccaaga cctcgcccga
      421 ggagtttccc gtggtcgtca tcggctgcgg cgaatcgggg ctgctggccg gaattcgctt
      481 gaaggaagcc gggattccgt tcaccatcat cgagaagaac gccggcgtag gaggtacctg
      541 gtaccagaac acctaccccg gggcgcgggt cgacgtgccc aaccacttct actgctacag
      601 cttcgagccg accgatcagt ggacacactt cttcgccgaa cagcctgagc tgcaggcgta
      661 tttcgagcgc gtgatggaca agcacaacat ccgcggccat gtgctgtggg aaaccgaggt
      721 cactgaggcg tgctgggacg acgccaccgc cacgtggacg gttcgtgccc gcgaccgcag
      781 cgggaggacg acagaactgc cggcgcgtgc ggtgatcacg gcggtgggtc agcttgaccg
      841 agcgcacaca ccgacgatcg aaggccagcg ggatttcgcg ggaccgacgt tccactccag
      901 cgcgtgggat cactccgtgg acctgcacgg caagcgcgtc gcgatgatcg gcgcgggggc
      961 gagcggcttc cagatcgctc ccaccatcgc acccgatgtc gaacacctca acgtgtttca
     1021 acgcaccgca cagtggatgt tcccgaaccc cagctaccac gaaaaggtgg gccagggtgt
     1081 gcaatgggcg ctgcgacatc tgccgttcta cggccgctgg tatcgcttcc tgttgttctg
     1141 gccgtggtgc gacaaggggc tggacgtggc acgcgtcgac ccgaattatc ccgatcagca
     1201 gacggcgatc aacgagctca gcgagatcac ccgggtcatg ttcaccgagt ggatcaccag
     1261 tcagatcggc gacgactccg acctgcgagc caaagtggtc cccgactatc cggcgaccgg
     1321 taagcgcacg ttgcaggaca acggcagctg gctgaagatg ctgacgcgcg acaacgtcga
     1381 gctggtccgg accgagatcg accacatcga atccggcgcg gtcgtcaccg aggacggcac
     1441 acgtcattcc gccgatgtga tcgtgtacgc caccggtttc gaggcaacca agctgttgtg
     1501 gcccatgacc atccgcgggc gcggcggaga agtactcgcc gagcgctggg gtgagcggcc
     1561 gtcggcgtat ctgggcat
//
LOCUS       DASYLX010001169         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3279762,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001169 DASYLX010000000
VERSION     DASYLX010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3279762"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VGP27_27660"
     CDS             complement(<1..609)
                     /locus_tag="VGP27_27660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7855108.1"
                     /translation="MTHPVLLVHGLGGTKSSWSFVARALAAKGLAVDAITYTPFGTSV
                     EQLADRLVVEVERTLSQTGADKVHLIGHSLGGVVIAQAITGGRLAGKVDTIVTLGAPF
                     GGSPWAHLVPFGAIVRALRQGSPLLRRLASAPVPEGVRWLAITATLDMIVPGVRSVPA
                     HAEVKTVAIGGVGHLGMLLSPEVVGSIVAAFPQIADEDNASDS"
     gene            1358..1600
                     /locus_tag="VGP27_27665"
     CDS             1358..1600
                     /locus_tag="VGP27_27665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855109.1"
                     /translation="MPTQAEVNGLWGDIRLKNLDTLVGRNVVGAVLVHCIYTCTAISE
                     AHPMMHVDGNPVRDIEERDDVALVDADTNRDLERPR"
     gene            1778..1924
                     /locus_tag="VGP27_27670"
     CDS             1778..1924
                     /locus_tag="VGP27_27670"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7855110.1"
                     /translation="MAEAARLAEVLATKNPTAVISSPLQRAVATAEAIGAAAGVPASI
                     DHPP"
BASE COUNT          467 a          753 c          766 g          444 t
ORIGIN      
        1 cgaatcagat gcgttgtctt cgtcggcgat ttgtgggaat gcggcgacga tgcttccgac
       61 aacctccggg ctgaggagca ttccgaggtg gccgacgccg ccgatagcca cggttttcac
      121 ttcggcatgg gcgggaaccg accggacgcc gggcacgatc atatcgagtg tggccgtgat
      181 tgccagccag cgcacgcctt cgggtaccgg cgcggaggcg agtcggcgca agagtggcga
      241 accttgccgc agcgcccgga caattgcccc gaacggcaca agatgcgccc agggcgaacc
      301 gccgaacggc gcgccgaggg tgacaatcgt gtcgaccttc ccggcaagtc gaccgcctgt
      361 gatggcctgc gcgatgacga ccccacccag gctgtgtccg atcagatgca ccttatcggc
      421 gccggtttgc gacaacgtcc gctccacctc gacgacgagc cggtcggcca gctgctcgac
      481 cgaggttccg aacggtgtgt aggtgattgc gtccactgcc aagcctttgg cagccagggc
      541 tcgcgcgacg aaggaccagc ttgacttcgt gccccctaag ccgtgcacga gcagcaccgg
      601 gtgcgtcaag ggtgcggggt gggcccggac ctcttgaagc gcaccgaaca cccggtcgtt
      661 gccgccgccg accaggtgca gcgacaccat cgtaactacc gatgccatca gccccagttc
      721 ggatgcctgc accatcgcgg catgtccgat tctccggacc agcttgcggg cgcccccgaa
      781 cggtcccggc ggcaatattg catctcgctg tctcagcaca tcgttgggtg ccgactgttg
      841 cgtttcttcc atctgcacgt gcgacatgtc gaaccccttt gttgcgactc gtagtgcatt
      901 gagaatcgcg gtcggcggcc atggtgtcat cgacggcagc ggcaatccgt ggttctcgcc
      961 aaccagattc gcgtctgcct gctagccgga gccgccagtt ccggtcgagg tgccaggctt
     1021 tttgggcgag ctaaagtctt gcaggtagcc ggtatctcac acgggcgcca gcgggaacga
     1081 ctcttgctga atcgtatgca atcgttgcga cgagaatgcg gttactcgac gcaactcaag
     1141 agatgacaac gatcaccagc tcgacccgag tacaagaaac tcaaagtcca gatttagcaa
     1201 caactccgaa gggaagcgtg ttctacgcag tagaaccggc ggtgacgcag ttgttcaaca
     1261 tccgcaatga ggaccaggag gccgcagcat gagcaaccaa ccacggggac tcaggctgct
     1321 ctgcccacaa ggctgtggaa cgactctcac cgtatcgatg ccaacccagg ccgaggtgaa
     1381 cggactatgg ggtgacatca ggctcaagaa tctggatacc ctcgtcgggc gcaacgtcgt
     1441 cggcgccgta ttggtgcatt gcatctacac gtgcaccgcc atttcggagg cccaccccat
     1501 gatgcacgtg gacgggaatc ccgtgcgtga catcgaggaa cgcgacgacg tcgcactcgt
     1561 cgacgccgac accaaccgag atctggagcg accccggtaa ctgatccacc gcgagtgagt
     1621 gtcggctatt ggtgggcgcc ggcgccggcg tagctagtcg aggtcaacgg gctgaggtcg
     1681 tccgctggcc ggccggaagg gaactgccgt gggaccgatc gcgaggctat gacatcggcc
     1741 gtcttggttg tctcacagcg ggcaccgaga actgttggtg gccgaggcgg ctcggcttgc
     1801 cgaagtgctt gccaccaaga acccgacagc agtgatctcc agcccgcttc agcgggcggt
     1861 tgccaccgca gaggcgatcg gtgcggcggc cggtgtgccg gcgtcgatcg accatccgcc
     1921 ttaacgaccg cgattacgga acgctgacag gagagcgtcg cgatgaggtg cgacgccagt
     1981 tcggcagcat cgacgccgcg cccggagttg aacccacttg acgttctggc ctcgcgtgct
     2041 tgcgcagcgt ttatcgagtt ggtggtcgaa cacgacactg gccctctcgt cctggtgtct
     2101 cacgatgcat tcaactgcgc attgctccct gaccgatccc tcattgaccg acatcgagca
     2161 aaggaccggc tgctggaacc agctgagctt cgtcggcggt gtatggcgcg tcgacgccta
     2221 caacctgaaa cccagtacag gagtggcgtt ttgaactcgg atacggaccg aactggcgcc
     2281 tgggtcgctt agagccttgg agcgccatgt ggccggaagt ggtagtggtg ttagcccttc
     2341 gctgggcgct aacaggtgat cacagattcc ggctggcatg ctcgaccgaa tgaccttgca
     2401 gtttcacaat cggtaggtga acgctctcag
//
LOCUS       DASYLX010001170         4452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3391866,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001170 DASYLX010000000
VERSION     DASYLX010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391866"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1060)
                     /locus_tag="VGP27_27675"
     CDS             complement(47..1060)
                     /locus_tag="VGP27_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7855111.1"
                     /translation="MLAESLEPTGPEDAGAAADAFLADAQRFRRELLAHCYRMTGSLH
                     DAEDLVQDTYLRAWKSYKGFEGKSSVRTWLYRIATNTCLTALEGRGRRPLPSGLGQPA
                     SDPTGDIAEHREITWLEPLPDSLKEDPSDPSVIVESRESVRLAFVAALQHLSARQRAV
                     LVLREVLQWKAAEVGEAIGASTAAVNSLLQRARAQLDAAQPTQDGEIADPESPEAKDQ
                     LAKYIEAFETYDMDKLVALFTEDAVFEMPPFDGWYQGPANIVALSKTHCPAEGPGDMR
                     FIAITANGQPAAALYMLNRETGLHEEFQLHVLEVRPGGIAHVVAFHAGDGLFGKLGLP
                     ASL"
     gene            1137..1979
                     /locus_tag="VGP27_27680"
     CDS             1137..1979
                     /locus_tag="VGP27_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7855112.1"
                     /translation="MASSRHERSFEGAGGVRIVYDVWEPEDEDCRGVVVLCHGYAEHA
                     RRYDHVARRFGESGLAVYALDLRGHGRSGGKRVYLRDISGYTDDFHTLVGIAAAEYPD
                     LKRIVLGHSMGGGVVFAYGVEHPDDYAAMVLSGPAVDAQDGQMPVKLLLAKVLGKVLP
                     GLPVEQLPTDAVSRDPEVVAAYNADPLVFHGKFPAGIARALIGVGETVPQRAPGLTAP
                     LLVVHGEQDKLVPVKGSRRLMEYVGSEDAHLTVYPGLYHEVFNEPERAVVLDDVISWI
                     EAKI"
     gene            1976..2968
                     /locus_tag="VGP27_27685"
     CDS             1976..2968
                     /locus_tag="VGP27_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855113.1"
                     /translation="MKVVAALVLSLLLLLSAGCSSDQKPQWVEDEVSFDADGLTIHGT
                     YRHQQKDAAPGPAALLISESGNTDRNGDNIVVGKIGNMRQLAELLSDRGVASLRYDKV
                     GTGKTGLGPYQQRPTDVGSSVYTTGAQAAARFLAGQAGTDKGRLSVYAVGEGAVHAIT
                     LASDTSPGAPKIHSLGLLQPLSGRYLDIITNRVRSDASPETLNTWLVAVDEVRTKGTV
                     PKDLPEGLGAILNPGNAKAIEEADKIDPVALAAKIPAGTPVLLTCSDTDRQALCNTMK
                     PLIDALKHTALTVVELKGVNHVLRDDPTDSIANLAKDAPLSPQVVTALDGFVAK"
     gene            2986..3735
                     /locus_tag="VGP27_27690"
     CDS             2986..3735
                     /locus_tag="VGP27_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEV7855114.1"
                     /translation="MTDDRMLARIAALLRQAEGTDNAHEAEAFMAAAQRLATATSIDL
                     AVARSHADQRTKAQTPVQRTITIGNAGTRGLRTYVQLFTVIAQANDVKCDIASNSTFV
                     YAYGFAEDIDASHALYAGLVTQMVKASEAYIASGAHRPTPTITARINFQLAFGARIGK
                     RLAEAREQAFREATKSKTRQPGTAIALRDKDIELKDFYRETSQARGSWRASSATAGYS
                     SSARRAGDRAGRRARLGNSQEINGSRTALNR"
     gene            3732..4226
                     /locus_tag="VGP27_27695"
     CDS             3732..4226
                     /locus_tag="VGP27_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04338 family metallohydrolase"
                     /protein_id="HEV7855115.1"
                     /translation="MTARDTQRSKVYAAEEFVRTLFDRAGERGNRTIEFFGTNLTLPP
                     EARFSSAETVQRYVDDVLALQSVRARWPDAGPLSTRRRRGTTAAHYERDEGGAAIAVP
                     ERRATWALRELVVLHELAHHLCDADPPHGPQFVATYCELAEAVMGPEVAHVLRVVYAK
                     EGVR"
     gene            4304..>4452
                     /locus_tag="VGP27_27700"
     CDS             4304..>4452
                     /locus_tag="VGP27_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV7855116.1"
                     /translation="MTEPDPKALDELFNRVLHTEDDALRAARESAAAADMPAIEVSAQ
                     HGRLLS"
BASE COUNT          754 a         1522 c         1509 g          667 t
ORIGIN      
        1 cggcggatgt cgacggatgt cgtcatgcac ccagcgtacg gacggctcag agggaggccg
       61 gcaggcccaa tttcccgaac aggccgtcgc cggcatggaa ggccaccacg tgcgcgatgc
      121 cgcccggacg cacctcgagc acgtgcaact ggaactcctc gtgcaagccg gtctcgcggt
      181 tgagcatgta cagcgccgct gcgggttgac cgttcgcggt gatggcgatg aaccgcatgt
      241 cgcccgggcc ttcggcgggg cagtgcgtct tcgacagcgc gacgatgttc gccgggccct
      301 ggtaccagcc gtcgaagggc ggcatctcga aaaccgcgtc ctcggtgaac agggcaacca
      361 gcttgtccat gtcgtaggtc tcgaacgcct cgatgtactt ggcgagctga tccttggctt
      421 ccggcgattc cggatcggcg atctcaccgt cttgtgtcgg ctgggcggcg tcgagttgcg
      481 cgcgcgcccg ctgcagcagg ctgttcacgg cggccgtcga tgcgccgatg gcttcaccca
      541 cctcggcggc cttccactgc agcacctcgc gaaggacgag taccgcgcgc tgccgtgccg
      601 agaggtgctg gagagccgcc acgaatgcca gccgcaccga ttcgcgggac tcgacgatca
      661 ccgatggatc ggacgggtcc tccttcagcg agtcggggag cggctccagc catgtgatct
      721 cgcggtgctc ggcgatgtcg ccggtcgggt ccgacgccgg ctggccgagc ccggacggca
      781 gtggtcgtcg ccctctgcct tccagcgcgg tcaggcaggt gttggtcgcg atccggtaca
      841 gccaggtccg cacggaggat ttgccctcga accccttgta ggacttccag gcgcgcaggt
      901 acgtgtcctg caccaggtct tcggcgtcgt gaagggaacc ggtcatgcgg tagcaatgcg
      961 cgagcagttc ccgccgaaac cgctgggcat cggcaagaaa tgcatccgcg gcggctcccg
     1021 catcctccgg gccggtgggt tccaggcttt ctgccagcac gctcacgccg tcgagcttac
     1081 gcagcgcccc gacaacttcc acggaccgat ttcgacgggt tggctagtct gcgctgatgg
     1141 ccagtagccg acacgagcgg tccttcgagg gcgccggagg cgtccgcatc gtctacgacg
     1201 tatgggaacc ggaggacgag gactgtcgcg gcgtggtggt gctgtgccac ggctacgccg
     1261 aacacgcccg ccgctacgac cacgtcgctc ggcggttcgg cgagtccggc ctggctgtgt
     1321 atgccctcga cctgcgcggc cacggccgct ccggcggcaa gcgggtctac ctccgcgaca
     1381 tctccggata caccgacgac ttccacaccc tcgtcggcat cgcggccgcg gagtacccgg
     1441 acctcaaacg gatcgtgctc ggccacagca tggggggcgg cgttgtgttc gcctacggcg
     1501 tcgagcaccc cgacgactac gccgcgatgg tgctgtcggg acctgcggtg gacgcccagg
     1561 acgggcagat gccggtgaag ctgctgcttg cgaaggtcct gggcaaggtg ctgccgggcc
     1621 tgcccgtcga gcagctgccc accgacgccg tgtcccgcga tcccgaggtg gtggccgcct
     1681 acaacgccga ccccctggtc tttcacggca agttcccggc aggcatcgcg cgagcgctga
     1741 tcggcgtcgg cgagacagtg ccgcagcgcg cgcccgggtt gacggcgccg ctgcttgtcg
     1801 tgcacggcga gcaggacaag ctggttcctg tgaagggcag caggcgtctg atggagtacg
     1861 tcggatccga ggacgcgcac ctgacggtct acccggggct ctaccacgag gtgttcaacg
     1921 agcccgagcg tgccgtggtg ctcgacgacg tcatatcgtg gatcgaggcg aagatttgaa
     1981 agtcgttgcc gcactggtac tttctctgct tctcctgctg tcggccggct gctcgtcgga
     2041 ccagaagccg cagtgggtcg aggacgaggt gagcttcgac gcggacggcc tcaccatcca
     2101 cggcacctac cgccaccaac agaaggacgc ggcccccggc cccgccgcgc tactgatctc
     2161 cgaaagcggc aacaccgatc gcaacggcga caacatcgtc gtcgggaaga tcggcaacat
     2221 gcgtcagctc gcagaactgc tgtcggaccg cggtgtagcc agcctgaggt acgacaaggt
     2281 cggcaccggc aagacggggt tgggccccta tcagcagcgg cccaccgatg tcggcagcag
     2341 cgtctacacc accggagccc aggctgccgc acgcttcctc gccgggcagg cgggcacgga
     2401 caagggccgg ctgtcggttt acgcggtggg ggagggcgcc gtgcacgcga tcacgctggc
     2461 gtcggacacc tcgcccggcg cgccgaagat ccattcgctg ggcctgctgc aaccgttgtc
     2521 gggccgctac ctcgacatca tcaccaaccg ggtgcgctcg gacgccagcc ccgagacgtt
     2581 gaacacctgg ctggtcgccg tcgacgaggt acgcaccaag ggcaccgtgc ccaaggacct
     2641 gccggaaggg ctcggcgcca tcctcaaccc gggcaacgcg aaggccatcg aggaagcgga
     2701 caagatcgac ccggtggcgc tggccgccaa gattcccgcg ggcacgccgg ttttgctgac
     2761 gtgctcggac accgaccgcc aggcattgtg caacaccatg aagccgctca tcgacgccct
     2821 caagcacacg gcgctgaccg tcgtcgagct caagggcgtc aaccacgtgc tgcgcgacga
     2881 cccgaccgac agcatcgcca acctcgcgaa ggacgcgccg ctttctccac aggtggtgac
     2941 cgcactcgac ggatttgtcg ccaagtagtt ttagtctgtt gggcgatgac tgacgacagg
     3001 atgctggccc gcatcgccgc gctgctgcgc caggccgagg gcaccgacaa cgcgcacgag
     3061 gcggaggcct tcatggccgc cgcgcaacgg ctggccaccg cgacgtcgat cgatctcgcc
     3121 gttgcgcgtt cgcatgccga ccagcggacc aaggcacaga cgcccgtgca gcgcacgatc
     3181 acgatcggca acgcgggcac cagggggctg cggacctacg tgcagctgtt caccgtgatc
     3241 gcgcaggcca acgacgtgaa gtgcgacatc gcgtccaact cgacattcgt ctacgcctac
     3301 gggttcgccg aggacatcga cgccagccat gcgctctacg cgggcctggt gacgcagatg
     3361 gtgaaggcgt cggaggccta catcgcctcg ggtgcgcacc ggcccacccc gacgatcacc
     3421 gcgcgcatca acttccagct ggcgttcggc gcgcgcatcg ggaagcggtt ggcggaggca
     3481 cgcgagcagg cgtttcggga agcgaccaaa agcaagacgc gccagccagg caccgcgatc
     3541 gcattgcggg acaaagacat cgagctcaaa gacttctatc gcgagacatc gcaggcccgc
     3601 ggcagctggc gagccagcag cgcgaccgcc ggttactcgt cgtctgcgcg gcgggcgggt
     3661 gaccgcgcgg gccgacgcgc ccggctcggt aacagccagg agatcaacgg ctcgcgcacc
     3721 gcgctgaacc ggtgaccgcc cgcgataccc aacgctcgaa ggtctacgcc gccgaagagt
     3781 tcgtccggac gttgttcgac cgtgccggcg aacgcggcaa ccgcacgatc gagttcttcg
     3841 gcaccaacct caccctgccg ccggaagcga ggttctcctc cgctgagacc gtgcagcgct
     3901 acgtcgacga cgtactcgcg ctccagtcgg tgcgggcacg gtggcccgac gccggtccac
     3961 tgagcacgcg acgacggcgc gggaccaccg ccgcgcacta cgaacgtgac gaaggcggcg
     4021 ccgcgatcgc cgtgcccgag cgacgggcca cctgggcgct ccgcgagctc gtcgtgttgc
     4081 acgagctggc ccaccacctg tgcgacgccg acccgccgca cgggccgcag ttcgtcgcga
     4141 cctattgcga actcgccgag gcggtgatgg gcccggaggt ggcccacgta ctgcgggtgg
     4201 tctacgccaa ggaaggtgtg cggtagtcgg gcgacgatca agcggcgatg tacgagccgc
     4261 gttgagaagg ccgacaatcc agccgagcgg taacgtcgga agcgtgaccg agccggaccc
     4321 gaaagccctt gatgagctgt tcaatcgggt gttgcacacc gaggacgacg cgctgcgcgc
     4381 cgcgcgggaa tcggctgcgg cggcggacat gcccgccatc gaggtgtcgg cccagcacgg
     4441 caggctgctg tc
//
LOCUS       DASYLX010001171         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2405512,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001171 DASYLX010000000
VERSION     DASYLX010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405512"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGP27_27705"
     CDS             <1..197
                     /locus_tag="VGP27_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855117.1"
                     /translation="VERLAAAQAADGSALRDIWLLRLRALLARTRGDDAAYRDLVGRY
                     RAMAESFGFEGHVDWAKAMM"
     gene            194..>1840
                     /locus_tag="VGP27_27710"
     CDS             194..>1840
                     /locus_tag="VGP27_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7855118.1"
                     /translation="MTAVVTCASCGNGLRENARFCDACGVPAGVPVDTAQYKQVTVLF
                     ADVVRSMHIAAAVDGERFRQIMTETMERSAKVVHRYGGTVEQTGDGVMALFGAPVALE
                     DHAFRACLAALAIQDEAGRLAAEVARHDGVALQLRVGLNSGRVIAGDTGSGSLGYAAT
                     GEAIGYARRMESAARPGEVLLSESTARLVEHAATLAEPEWVRIKGTDEPVQARRLLAT
                     GQRRDGAGRAEARLIGRGWEMAVLDAIVERAINARGGVVNVVGPAGIGKSRSARETAA
                     LAASRGVEVNWAFCESHASDIPFQAVARLLRESTGVTALDGKAARARLRAMLPGTDAQ
                     DLLLFDDLLGVSDGDVPLPLIDPDARRRRLTALINTATLARTEPALFVIEDAHWIDAV
                     SESMLADFLAIIPRTPIMVLITSRPEYDGALSRVPGAQTIALAPLGDSDTRVLLAELL
                     GADPSLNELKAVIAERAAGSPFFAEEMVREFVQGGVLIGEHGDYVCRTNVAEVSVPAT
                     VQAAIEARIDRLTAPAKRTLNAASVVGAHFGADLLAALGLD"
BASE COUNT          299 a          545 c          668 g          328 t
ORIGIN      
        1 cggtcgaacg gttggcggcg gcgcaagccg ccgacggttc ggcgctgcga gacatctggt
       61 tgttgcggct gcgtgcgctg ctggcgcgga cccgcggcga cgatgccgcc taccgggatc
      121 tggtcggtcg ctatcgcgcg atggcggagt cgttcggctt cgaaggccac gtcgactggg
      181 cgaaggcgat gatgtgacag ctgttgttac gtgcgcatca tgcggcaacg gcctgcggga
      241 gaacgccaga ttctgcgacg cctgcggtgt accggccggg gttccggtcg atacagcgca
      301 gtacaagcag gtgacggtgc tgttcgcgga tgtcgtccgc tcgatgcata tagcggccgc
      361 cgtggatgga gagcggttcc gtcaaatcat gaccgaaacg atggagcggt cggcgaaagt
      421 agtgcaccgc tacggcggga cagtggaaca aaccggtgac ggggtgatgg cgcttttcgg
      481 cgccccagtt gctctggaag atcacgcttt tcgggcgtgc ctcgccgcgc tcgccattca
      541 ggacgaggcg ggccggttgg cggccgaggt ggcgcgccac gatggtgtgg ctctgcagtt
      601 gcgggtgggc ctgaattcgg gtcgggtcat cgccggtgac acgggttcag gttcattggg
      661 ttacgcggcg accggcgagg caatcggtta cgcccggcgg atggaatcgg cggcgcgacc
      721 cggcgaggtc ttgctgtcag agtcgaccgc tcgacttgtc gagcacgccg ccacgctcgc
      781 cgaaccagaa tgggtgcgca tcaagggaac cgacgaaccg gtgcaagcac gccgattatt
      841 ggcgacgggc caacggcgtg acggggccgg ccgcgccgag gcgagactga tcggtcgggg
      901 ctgggaaatg gcggtactcg atgcgatcgt ggaacgcgcg atcaatgccc gcggtggtgt
      961 cgtgaatgtg gtgggaccgg ccggaatcgg caagagccgt tcggcgcgcg agactgcagc
     1021 gctggcggcc agccgtgggg tcgaggtgaa ttgggccttt tgcgagtcac acgccagcga
     1081 cattcccttc caggcggtgg cgcggctgct ccgcgagagc accggcgtga ccgccctcga
     1141 tgggaaggcc gcgcgggctc ggctgcgagc gatgcttcca ggcaccgacg ctcaggatct
     1201 actgcttttc gatgatctat tgggtgtctc cgacggagat gtcccgctgc ccctgatcga
     1261 ccccgatgca cggcggcgcc ggttgaccgc cctgatcaac actgcgacgc tggctcgcac
     1321 cgaaccggcg ctgttcgtca tagaggacgc gcattggatc gatgcggtca gcgaatcgat
     1381 gttggccgac ttcctcgcca tcattccgcg cacaccgatc atggtgctga tcacgtctcg
     1441 ccccgaatac gatggagcgc tgtcacgagt acccggcgct cagacgatag cgcttgcccc
     1501 actcggtgat tcggatacca gagtcctgct cgccgagctg ctgggagcgg atccctcgct
     1561 caacgagttg aaggcggtca tcgcagagcg ggccgcggga agcccgttct tcgccgagga
     1621 aatggtgcgt gagttcgtcc agggaggggt gctgattggc gaacacggtg actacgtctg
     1681 tcgcacgaat gtcgccgagg tgagcgttcc ggccacggtt caggcagcca tcgaggcacg
     1741 catcgaccgg ctgaccgctc ctgccaagcg gacgttgaac gcggcgtcgg tggttggagc
     1801 ccacttcggg gcggacctcc ttgcggcact ggggctcgat
//
LOCUS       DASYLX010001172         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1087830,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001172 DASYLX010000000
VERSION     DASYLX010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087830"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..495
                     /locus_tag="VGP27_27715"
     CDS             91..495
                     /locus_tag="VGP27_27715"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7855119.1"
                     /translation="MFVEAASTPILVTSASATRTHSLNSDPRWRLLVAGEDTVDVDVA
                     IKQLGGLGMQRILCEGGPTLLDQLIDADVVDEICVTIAPLLAGSQPVGHRTPSRQSVP
                     ASMELRHALLCDSYVFLRYRRANKQSDVRGSQ"
     gene            complement(730..1635)
                     /locus_tag="VGP27_27720"
     CDS             complement(730..1635)
                     /locus_tag="VGP27_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855120.1"
                     /translation="MARILLQTTIAAHPDDWDVSRFSLLAAELRGAGHEVTARNRCGG
                     AGDDPVLAHLDDLGFDQLWLLAVDVGDGLTAAEADSLTRFREAGCGVLTARDHQQLGS
                     CLVRLGSLGRVNRFHDESVDSEAMCDDRGTPEISWPNFHSGANGDYQPVLCNGSWHEL
                     LHTERTASGRIEWFPAHPHEGLVTPEGPHATVVAQGCSRATGRPFNLAVALDGESAPD
                     GRPMGRAVAESTFHHFADYNWDLGCGAPSFVSEPPGGEIRADGTRLTVFKDYVRNLAA
                     WLQPAACRGCRLGVDGFAETPGSLS"
     gene            complement(1645..2079)
                     /locus_tag="VGP27_27725"
     CDS             complement(1645..2079)
                     /locus_tag="VGP27_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7855121.1"
                     /translation="MSSAYDPQWENALTVVQEVEPPLIPADAHVMTVTITYPPGSGGA
                     PPHRHPSGPAFGYVLEGELLFEVEGEPPRVVRAGEAFWEAGGDVIHYSDANNRDDVRL
                     RFLVTMMCVPGQPMLTLVSEEELAARRHLRVMAPPVSDEVDG"
     gene            complement(2106..2861)
                     /locus_tag="VGP27_27730"
     CDS             complement(2106..2861)
                     /locus_tag="VGP27_27730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7855122.1"
                     /translation="MPSSAARDGADRFPELAGLTALITGGTAGIGRACAQLLAQAGAS
                     VVISGRDERRGRSCADELGVHYLKADLADLDSVQALVRLAGEVDVLVNNAAAFPSAMT
                     VAQDVTAFAWTFDTNVRGTYFLTAELAKGMLARGRGAIVNVTSMVASKGVPGASAYSA
                     SKAAAESLTRTWAVEFASRGVRVNSVAPGPTRTEGVVAEWGEVNDELGRALPMGRVAR
                     PEEIAEAVLFLASPRASFITGATLHVDGGGTAI"
     gene            complement(2839..3447)
                     /locus_tag="VGP27_27735"
     CDS             complement(2839..3447)
                     /locus_tag="VGP27_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7855123.1"
                     /translation="MRAADTLEVVGIGPASDPELAARFVTEAVPLFDALHRRARRLTY
                     CEADAEDLLQDTVLHAYQGFGSFREGTNLNAWLFRILHNRWVSRHRHRERRPAEVPLE
                     ITDGARRVPPTGCSAEVEVLDVMPNDDVKVAFAALPDGVRVAMYYVEVEGYTYAETAA
                     LMDVPMGTVMSRVSRGRQRLRVALSHMDHGREEFGDAEQRSA"
     gene            complement(3581..>5073)
                     /locus_tag="VGP27_27740"
     CDS             complement(3581..>5073)
                     /locus_tag="VGP27_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7855124.1"
                     /translation="AIAELAPLSELQRGQVVRMRAQLQFMRSRTGAPGAPRTSESAVL
                     LLSAARGFDGIDDDAARETYLEALTAAMYAGRLGEPGLLAVVAQAGRAACARIETPRR
                     PMDLLLSGMTSRILDGPGAGSAHLRDALESWNTDAQNAIGPVQYWPFPIAQESAAHEL
                     WDDAVLQQIATGMVRRARDGGAVAVLPSALVYRAGVHVYTGEFAEASALLAEADAITA
                     ATGYTPMKYHSLTLAAWRGVPAAAIELIEGAAADGLTKGEGRLIGVSRFTAAVLFNGL
                     SRYEQAYQSAQSCCEYEDLGFHGWCLFELIEAAVHLGERDSAEAALSRLEERAGSSGT
                     EWGLGAVAAARALLAPNEGAERLFAEALERLETAHIGPYVARTRLCYGEFLRRVNRRT
                     DARRQLSEAYEKFTAMGAEAFAERARRELTVTGKKVRKQPVASGAELTAQEAQIARLA
                     ADGLTNQEIGAQLFISAHTVEWHLRKVFLKLGITSRRQLRNTALSR"
BASE COUNT          851 a         1746 c         1607 g          869 t
ORIGIN      
        1 gcggctcagg aagcgctgcg tggagcagat cgcgggttcg cgccaccacc gatcgcagtc
       61 gtcagccaga ctggacatct cccgccgacg ttgttcgtcg aagccgcatc gacccccatc
      121 ctcgtgacca gcgccagcgc aacgcgaacc cattctctga acagtgaccc ccgctggaga
      181 cttctggtgg cgggcgagga caccgtcgat gtcgacgtgg cgataaagca gcttggtggc
      241 ctcggcatgc agcggatcct gtgcgagggc ggccctactc ttctcgatca actcatcgac
      301 gcggatgtcg ttgatgaaat atgcgtcacc atcgcgccgc tgctcgcagg cagtcaaccc
      361 gtcggccatc gcacacctag ccgacaatct gtccctgctt ccatggaact ccgccacgcg
      421 ctgctatgtg actcgtacgt cttcctcagg tacaggcgcg cgaataagca atctgatgtg
      481 cgtggatctc agtagtcccg actgggatcg aaccagtcac ggctaatgcg agcaccggca
      541 gatgaccagc tcaagtctgg tttggttgtt gggctcacac tgttctggct cacagcatct
      601 gaagctggga ccacttgaat ccctgtcaga gttcggccga aagcggttgg tatgcagagt
      661 atccgaacgg ctgccgacaa gaaggaaccg cggctcggcc cgtcgccggc catcgagcag
      721 aacggttggt caggaaagcg atccaggagt ctcagcgaac ccgtcgacgc ctagtcggca
      781 gccgcggcag gccgccggtt gcaaccacgc ggcgagattc cgcacatagt ccttgaacac
      841 ggtcagcctc gtcccgtcgg ccctgatttc gcctcccggc ggttccgaca cgaaagacgg
      901 agcaccgcag ccgaggtccc agttgtaatc ggcgaagtga tgaaacgtgg actcggcaac
      961 ggcgcggccc atcggacggc cgtccggcgc ggactcgccg tccaaagcca ccgcgaggtt
     1021 gaacggccga cccgtggctc ggctgcatcc ttgggcgacg acggtcgcat ggggaccttc
     1081 gggtgtcacc aagccctcgt gaggatgagc cggaaaccac tcgatgcgcc cactcgcggt
     1141 ccgctcagtg tgcagcagtt cgtgccatga cccgttgcac agcaccggtt ggtagtcccc
     1201 gttggcaccg gaatgaaagt tcggccagga gatctcaggt gtgccgcggt cgtcgcacat
     1261 ggcctccgag tcgacggatt cgtcgtggaa ccggttgact cggccgagcg agccgagccg
     1321 caccagacag ctgcccaact gctggtggtc gcgagcggtc agcacaccgc aaccggcttc
     1381 gcggaaccga gtcagtgagt cggcctcggc ggcggtgagg ccgtcaccta cgtcgacggc
     1441 aagcagccag agttggtcga agccgagatc gtcgaggtgg gccaagaccg gatcgtcacc
     1501 ggccccgccg caccggttgc gggcggtcac ctcgtgcccg gcgccccgca actcggcagc
     1561 cagcaggctg aagcgggata cgtcccaatc gtcgggatgt gctgcgatgg tcgtctgcaa
     1621 caggattcgc gccatcctcc gtccctaccc gtcgacttcg tccgacacag gcggtgccat
     1681 cacccgaagg tgccggcgcg ccgccaactc ctcctcgctg acgagcgtca gcatcggctg
     1741 gccaggaacg cacatcatgg tcaccaggaa ccgcagcctg acgtcatcgc ggttgttcgc
     1801 gtccgaatag tggatgacgt ctccaccggc ctcccagaac gcctcccccg cccggaccac
     1861 ccgcggtggc tcgccttcga cttcgaacag cagctcgcct tcgaggacgt agccgaaggc
     1921 cggcccgctc ggatgccggt ggggcggcgc gccgccgctc cctggagggt aggtgatggt
     1981 cacggtcatc acgtgcgcat cggctgggat cagcggtggt tcgacctctt gcaccaccgt
     2041 cagggcgttc tcccactgag gatcgtatgc actggacatc gatagtctcc tttcagctag
     2101 atcattcaga ttgcagttcc gccaccgtcg acgtgcagcg tggctccggt gatgaaactc
     2161 gcccggggag acgccaggaa cagcacggcc tctgcgatct cctcgggacg cgcgacccgc
     2221 cccatgggca aggcgcgccc caattcgtcg ttcacctcgc cccactccgc gacgacgccc
     2281 tcggtgcggg taggtcctgg agcgacgcta ttgacgcgca ctcctctcga agcgaattcc
     2341 accgcccacg ttcgggtcaa cgactcggcg gcggccttgg acgcgctgta cgccgaggcg
     2401 ccgggaacgc ccttcgacgc gaccatcgat gtcacgttga cgatggcacc gcgcccgcgg
     2461 gccaacatgc ccttggccag ttccgcggtc aagaagtacg tcccgcgcac attcgtgtcg
     2521 aaggtccacg cgaacgccgt cacgtcctga gcgacggtca tcgccgaggg aaatgccgcc
     2581 gcattgttga cgagcacgtc tacctcaccg gcgagccgta ccagcgcctg caccgagtcc
     2641 aggtcggcca agtcggcctt gaggtaatgg actcccaatt cgtcggcgca ggagcggccg
     2701 cgccgctcgt cccttccgct gatgacgacc gatgcgcccg cctgggccag cagctgggcg
     2761 caagcgcggc caatgcctgc ggtgccgccg gtgattagcg cggtgaggcc ggccagttct
     2821 ggaaaccgat cggcgccatc acgcgctgcg ctgctcggca tcaccgaatt cctcacggcc
     2881 gtgatccatg tgcgagagcg cgacgcgcaa ccgctgccgg ccgcgcgaca cccgcgacat
     2941 gacggtgccc atcggcacgt ccatgagcgc ggcggtctcg gcgtaggtgt acccctccac
     3001 ttcgacgtag tacatggcca cccgtactcc gtccggcagt gccgcgaacg cgaccttgac
     3061 gtcgtcgttg ggcatcacgt cgagcacttc cacctcggcg gagcagccgg tgggcggtac
     3121 tcgacgcgct ccgtcggtga tctcgagtgg cacctccgct gggcggcgct cccggtggcg
     3181 atgcctgctc acccatcggt tgtgcaggat ccggaacagc cacgcgttga ggttggtgcc
     3241 ttcccgaaag gatccgaagc cttgataggc gtgtaacacc gtgtcctgca ggagatcctc
     3301 cgcgtcagcc tcgcagtagg tcagtcgacg tgcgcggcgg tggagcgcgt cgaacagagg
     3361 cacggcctcg gtgacgaagc gcgcggcgag ttcggggtcc gacgcgggcc cgatgcccac
     3421 cacctcgagt gtgtctgctg cccgcatgat cgaacctccc gctgcgccta cgtgccgtct
     3481 gtcggcgcgg attgcgccgt cgctactcag actcgtcgcg acgaggtcgt ggcggaccct
     3541 tggaagttcg tggtctcccc ctgaatccgt agtcggggcg ttacctagac agtgcggtgt
     3601 tgcgcagctg cctgcgtgac gtgatgccga gcttgaggaa gaccttgcgc aggtgccatt
     3661 cgaccgtatg tgcgctgatg aacagctgcg caccgatctc ctggttggtg agtccgtcgg
     3721 cggccagccg tgcgatctgc gcctcctgtg cggtgagttc ggctcccgag gccaccggct
     3781 gtttgcgcac cttcttcccg gtgaccgtca gttcccggcg ggcccgctcg gcgaacgctt
     3841 cggcgcccat ggctgtgaac ttttcgtagg cctcgctcaa ctgcctgcgg gcatcggttc
     3901 gccggttcac gcgacgcagg aactcgccgt agcacagccg ggtacgcgcc acgtacggcc
     3961 cgatgtgcgc ggtctcgagg cgttcgaggg cttccgcgaa aagcctctcg gcgccctcgt
     4021 tcggcgcgag cagagcgcgg gcggccgcta ctgcgcccaa cccccactcg gtaccgctgc
     4081 taccggcacg ctcttcgagc cttgacagcg ccgcctcagc cgagtcccgc tcgccgaggt
     4141 gtacagccgc ctcgatgagc tcgaagaggc accacccgtg aaagccgaga tcctcgtatt
     4201 cgcaacagct ctgcgcagat tggtaggcct gctcgtaacg gctgagaccg ttgaagagca
     4261 cggctgcggt gaacctgctg acaccgatca gccgtccttc gcccttggtg aggccgtccg
     4321 cagcagcccc ctcgatcaac tcgatcgcgg ctgcaggcac accgcgccag gcggccaggg
     4381 tcagcgagtg gtacttcatc ggggtgtagc cggtggcggc cgtgatcgcg tcggcctccg
     4441 cgagaagcgc actggcctcg gcgaactcgc cggtgtacac atggaccccc gcgcggtaca
     4501 ccagagctga cggcaacacg gcgaccgcgc cgccgtcacg cgcccgccgc accatgcccg
     4561 tggcgatctg ctgcagtacc gcgtcatccc acagttcgtg tgcagccgac tcctgagcaa
     4621 tcgggaacgg ccagtactgg accggaccaa tagcgttctg cgcatccgta ttccacgact
     4681 ccaacgcgtc gcgtaggtga gcagaccctg cgccagggcc gtcgagtatc cggctcgtca
     4741 ttccgctgag caatagatcc atcgggcgcc gcggcgtctc gattcgagca caggcggcac
     4801 ggccggcctg cgcgaccacg gccaacagtc caggctcccc cagtcggcct gcgtacatgg
     4861 cggcagtcag cgcttcgaga taggtctcgc gggccgcatc gtcgtcgatt ccgtcgaatc
     4921 ctcttgcggc actgagcaac aacaccgcgg actcgctggt tcgtggtgca cccggcgcgc
     4981 ccgtccggct gcgcatgaac tgtagttgcg cccgcatcct gaccacctga ccgcgttgca
     5041 gttccgacaa cggtgcaagt tcggcgatgg cga
//
LOCUS       DASYLX010001173         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_648357,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001173 DASYLX010000000
VERSION     DASYLX010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648357"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGP27_27745"
     CDS             <1..367
                     /locus_tag="VGP27_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEV7855125.1"
                     /translation="FWNGCRENKLLIQRCARCEQLRQPARPMCPTCHSLEWDTVESTG
                     RGTVYSYVMPQHPPMPLMEYPYIVALVELDEGVRLVSNLREIAPADVEVGMPVEVFYE
                     TFDTIDGKDIVLHQFRPVR"
     gene            367..1500
                     /locus_tag="VGP27_27750"
     CDS             367..1500
                     /locus_tag="VGP27_27750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7855126.1"
                     /translation="MDFTFTEDQETIGKVARQLFEHRATPEHLTELEGGEERHDADLW
                     RELARADLLGISLPESVGGSGRGFVELALLLTEVGWSVAPVPAYATLLLGADTIARYG
                     DDAQLQRYLPAVIDGSWLLTAGLAEPGRSDPTRPATTARRDGGCWRLDGTKELVPAAQ
                     LARTILIPASLGDGDIGVFLVDADSDGVEIRNVVTTNGEPHADVVLDGAATTDRLAGD
                     DREILESLYARGLVGLCAIQLGVTERALRMAASYTTEREQFGRPIGSFQAVQQRMADA
                     FIDVEAIRWTMWQAAWSLEQGGPPGRQAAIAKFWAAEAGSRVAATAQQVHGGIGIDTT
                     YPLFRYFLWAKHNELALGSSSAQLARLGATYVGVGCAREEQKG"
     gene            1503..1985
                     /locus_tag="VGP27_27755"
     CDS             1503..1985
                     /locus_tag="VGP27_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7855127.1"
                     /translation="MTTTKFRTTTLDWHDINVGDEVAPLEIPVTATVIVAGAIASRDF
                     MPVHHDPEFAKKQGSPNMFMNILTTNGLCVRFLTDWAGPEAMVKRLAIRLGVPAFPND
                     PLRFTGTVTGKTDGADGEGLIDVEFAGSNSLGDHVSGTATVSLLRTRGAMKDTDRADA
                     "
     gene            1982..>2230
                     /locus_tag="VGP27_27760"
     CDS             1982..>2230
                     /locus_tag="VGP27_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEV7855128.1"
                     /translation="MSPLSGVTAIAGIGQTEFSKESGRSELQLACEAVKAAIDDAGLQ
                     PSDIDGMVTFTMDSSDEIEIARNVGIGDLTFFSRVHHGG"
BASE COUNT          415 a          748 c          721 g          346 t
ORIGIN      
        1 cttctggaat gggtgccgcg agaataagct gctgatccag cgatgcgcca ggtgtgagca
       61 gctgcgccag ccggcgcggc cgatgtgccc gacatgtcat tcactcgaat gggacaccgt
      121 cgagtccacc ggtcgaggca ccgtctacag ctacgtgatg ccgcaacatc ctccgatgcc
      181 gttgatggag tatccctaca tcgtcgcgct ggtcgaactc gacgaaggag tgcggttggt
      241 gtccaatctg cgcgaaatcg cccctgccga cgtcgaagtc ggcatgcccg tcgaggtctt
      301 ctacgagacg ttcgacacca tcgacgggaa agacatcgtg ctgcaccagt tccgaccggt
      361 ccgataatgg acttcacctt caccgaagac caggagacga tcggcaaggt cgcgcgtcag
      421 ctcttcgagc accgcgccac ccccgaacac ctcaccgagc tcgaaggcgg cgaagagcgc
      481 cacgacgccg acctgtggcg cgaacttgcg agggccgacc tgctcggaat ctcgttgccg
      541 gagagcgtgg gtggcagcgg tcgaggcttt gtggagttgg cactgcttct gacagaggtg
      601 gggtggagcg tcgcgccggt gccggcctac gccaccctgc ttctcggcgc cgacaccatt
      661 gcccgctacg gcgatgacgc acagctgcag cggtaccttc ccgccgtcat cgacggctca
      721 tggctactca cagcgggatt ggcggagccc ggccgctccg acccgacccg accggcgacg
      781 acggcgcgac gcgacggcgg ctgctggcgc ctcgacggca ccaaggagct ggtacccgcg
      841 gcacagctgg cgcgcaccat cctcatccca gcctcgctcg gcgacggcga catcggcgtc
      901 ttcctcgtcg acgcggactc cgacggtgtc gagattcgca acgtcgtgac gactaacggc
      961 gaaccgcacg ccgacgtcgt cctcgatggt gcggccacca ccgaccgact tgccggcgac
     1021 gaccgtgaga tcctcgaatc actctatgcg cgtggacttg tcgggctgtg cgcgatccaa
     1081 ctcggcgtga cggagcgcgc gctccggatg gccgcgtcct acaccaccga acgcgaacag
     1141 ttcggaaggc ccatcggcag cttccaggcc gtgcaacaac ggatggccga cgcgttcatc
     1201 gacgtcgagg ccatccgctg gaccatgtgg caggccgcct ggtcactcga acagggtgga
     1261 ccgccggggc gccaggctgc tatcgcgaaa ttctgggcgg ccgaagcggg ctcgcgcgtc
     1321 gcggcgaccg cccagcaggt gcacggggga atcggcatcg ataccaccta ccccctgttc
     1381 cgctacttcc tgtgggccaa acacaacgag ctggcgctcg gctcgtcatc ggcgcaactt
     1441 gcacgcctcg gcgcgacata cgtaggagtc ggctgcgcac gcgaggagca gaaaggataa
     1501 ccatgacgac cacgaagttc cgcaccacca cactggactg gcacgacatc aacgtcggcg
     1561 acgaggtggc tccgctcgag atacccgtga ccgcaacggt catcgtcgcg ggcgccatcg
     1621 cgtcgcgcga cttcatgccg gtgcaccatg acccggagtt cgcgaagaag cagggctcgc
     1681 ccaacatgtt catgaacatc ctcaccacca acgggctgtg cgtgcggttc ctgaccgact
     1741 gggcgggtcc ggaggcgatg gtgaagcggc tggcgatccg gttgggtgta cctgcgttcc
     1801 ccaacgatcc gctgcggttt accggcaccg tcaccggcaa gaccgatggc gctgacggcg
     1861 aggggctcat cgacgtcgag ttcgcaggca gcaacagtct cggggatcac gtgtcgggaa
     1921 cggcgaccgt cagccttttg cgcacgcgag gagcaatgaa ggatacggac cgggccgacg
     1981 cgtgagcccc ctctcgggag tgaccgccat cgcgggcatc ggccagaccg aattctcgaa
     2041 ggagtccggc cgaagcgaac tccaactcgc ctgcgaggcg gtcaaagccg cgatcgacga
     2101 cgccgggctg cagccgagcg acatcgacgg catggtcacc ttcacgatgg actcgagcga
     2161 cgagatcgag atcgcgcgaa acgtcggcat cggcgacctg acgttcttca gccgcgtgca
     2221 ccacggcggt
//
LOCUS       DASYLX010001174         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2843122,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001174 DASYLX010000000
VERSION     DASYLX010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843122"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VGP27_27765"
     CDS             <1..1084
                     /locus_tag="VGP27_27765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7855129.1"
                     /translation="SVYTRRDDPDLPERVEAPQGYTVVHVPAGPAEQLPKDELLTHMG
                     PFAQYLHACWEADRPDVTHAHFWMSGIATQLAARHLGLPAVQTFHALGVVKRRHQGAN
                     DTSPKDRLRLEAMVARTATWVAATCTDEVFELMRMGRSRARISVVPCGVDNDLFRPEG
                     PVAEKSAKHRIISVGRFVPRKGFDDMVRAMPSIPNAELVIVGGPDRTELDSDPEARRL
                     RELAKQLGVGRRVHLLGSVAREDMPAMLRSADIVACTPWYEPFGIVPLEAMACGVPVV
                     ASAVGGMLDTVVHDVTGRLIPPRKPAEIAAAINPLLRDSFLRQALGLAGRDRVCARYS
                     WDRVAADTMRIYQTVAQVNYPRVSTG"
     gene            complement(1060..1971)
                     /locus_tag="VGP27_27770"
     CDS             complement(1060..1971)
                     /locus_tag="VGP27_27770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7855130.1"
                     /translation="MAAARTTFVIASRNRAAELSTTITRLLNTTACPIIAVDNGSEDN
                     SVAVIRRFANLSAGRVLAIELKSNRGVVARNIGVAACQTPNVAFCDDDSWWAPNAVEI
                     AEDIFDRHPTVGLLAARTVVLPQRRDDPMTAELANSALGRRADLPGPSILGFMTCAAI
                     VRKSAFLAAGGFSELLHFYGEEQLLAIDLAARGWDLCYCPALTAYHQPSAVRGSSSAR
                     QAREMRNAVLTTWMRRPMLPCLKATGRLVVAAMRDGEHALGAVQALMRLPSVVSQRRR
                     LPEEVERALTLLESDKTHGGHPVDTRG"
BASE COUNT          320 a          656 c          685 g          316 t
ORIGIN      
        1 gtcggtctac accaggcggg acgacccgga cctgcccgag cgggtcgagg cgccgcaggg
       61 ctacacggtc gtgcatgtgc ctgccgggcc ggccgagcaa ctgcccaagg acgagcttct
      121 cacccacatg ggcccgttcg ctcagtatct gcatgcctgc tgggaggccg accggccaga
      181 tgtgacgcat gcacacttct ggatgtcggg tatcgccacc caactggcgg cgcggcacct
      241 ggggttgccc gcggtgcaga ccttccacgc gctcggtgtg gtgaagcgtc gtcatcaggg
      301 tgccaacgac accagcccca aggaccggct gcggctggag gcgatggtgg cgaggaccgc
      361 gacctgggtg gcggccacgt gtaccgacga ggtcttcgaa ttgatgcgca tgggcaggtc
      421 gcgcgcccgg atctccgtgg ttccgtgcgg ggtcgacaac gacctgttca ggcccgaggg
      481 cccggtggcc gagaagagcg ccaagcaccg catcatcagc gtgggaagat tcgtgccgcg
      541 caagggcttc gacgacatgg tgcgggcgat gccgtcgatc cccaatgccg agttggtgat
      601 cgtcggcggc ccggaccgga cggagttgga ctccgatccg gaggctcgcc gactgcgcga
      661 attggcgaag caactcggtg tcggccgccg cgtccatctc ctcggttcgg ttgcgcgtga
      721 ggacatgcct gcgatgctgc gttctgcgga catcgtcgcg tgcacaccgt ggtacgagcc
      781 gttcggcatc gtgccgctgg aggccatggc gtgcggtgtc cctgtggtgg cctccgccgt
      841 cggcggcatg ttggacaccg tcgtccacga tgtgaccggg cggttgatcc ccccgaggaa
      901 gcccgcggag atcgccgccg cgatcaaccc gctgctgcgt gattcgtttc tccgtcaagc
      961 cctcggattg gcagggcggg accgggtgtg cgcccgctac tcgtgggacc gggtcgcggc
     1021 cgacaccatg cgcatctacc agacggtggc gcaggtgaac tatccgcggg tgtcgaccgg
     1081 atgaccacca tgggttttgt cggactcgag cagggtcaac gcccgctcga cctcctcggg
     1141 aagccgcctg cgttggctga cgaccgaagg caggcgcatc aacgcctgca ctgcgccaag
     1201 cgcatgctcg ccgtcgcgca tcgctgccac gaccaaccgg ccggttgcct tgaggcacgg
     1261 caacatcggc cgtcgcatcc acgtggtgag caccgcgttg cgcatctcac gggcctggcg
     1321 ggccgacgaa ctgccgcgca ccgcagacgg ctgatggtat gcggtcaacg cggggcagta
     1381 gcacaagtcc cagccgcgcg cggcgaggtc aatggcgagc agctgctcct caccgtagaa
     1441 gtgcagcagc tcactgaacc cgcccgccgc aaggaaagcc gacttgcgga cgatcgccgc
     1501 gcaggtcatg aagcccagga tcgacgggcc gggcagatcc gcccgtcggc ccagtgcgct
     1561 gttggccagc tcggcggtca tcggatcgtc gcggcgctgc ggcaggacga cggtgcgcgc
     1621 cgccagcaga ccgacggtcg gatgccggtc gaagatgtct tcggcgatct cgacagcatt
     1681 cggcgcccac cacgaatcat cgtcgcagaa agcaacattg ggtgtctggc acgccgccac
     1741 cccgatgttg cgagccacca cgccccgatt ggacttcagc tcgattgcca gcacacggcc
     1801 ggccgacagg ttcgcgaagc gccggatgac cgcgaccgaa ttgtcctcgg atccgttgtc
     1861 cacggcgatg atggggcacg ccgtcgtgtt gagcagccgc gtgatcgtcg tggagagctc
     1921 tgccgcccgg ttgcggctgg cgatcacgaa cgtcgttcgg gcggcagcca taaccga
//
LOCUS       DASYLX010001175         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1088781,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001175 DASYLX010000000
VERSION     DASYLX010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088781"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGP27_27775"
     CDS             <1..222
                     /locus_tag="VGP27_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510380.1"
                     /note="catalyzes the formation of precorrin 6x from
                     precorrin 5; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HEV7855131.1"
                     /translation="GFAGTAVIMLDGDCAFRACSPDTRIWWGAYLGTPDELLVSGTVG
                     EVGKQIVEMRAEARARHGWIMDTYLLRSP"
     gene            266..667
                     /locus_tag="VGP27_27780"
     CDS             266..667
                     /locus_tag="VGP27_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV7855132.1"
                     /translation="MVAFLLRAALTGFALWIVSLVVSGVEFVGGDTTLQRVGIILVVA
                     VIFGLVNAIIKPIVQFVSIPLYILTLGLFHIVVNALMLWITSRITENTTHWGLYIDDF
                     WWTAIWAAVVLSIVSWALSLVVRDTAGVPRG"
     gene            684..926
                     /locus_tag="VGP27_27785"
                     /pseudo
     CDS             684..926
                     /locus_tag="VGP27_27785"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            1089..1547
                     /locus_tag="VGP27_27790"
     CDS             1089..1547
                     /locus_tag="VGP27_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEV7855133.1"
                     /translation="MAALGPDALGLTADGLAEVLAGQSGRIKTVITDQKVIAGIGNAY
                     SDEILHVAKLSPFATAGKLTDAQLASLHDAMTSVLTDAVTRSVGQQAATLKGEKRSGL
                     RVHARTGMPCPVCGDTVREVSFADKSFQYCPTCQTGGKVLADRRLSRLLK"
     gene            complement(1540..>1873)
                     /locus_tag="VGP27_27795"
     CDS             complement(1540..>1873)
                     /locus_tag="VGP27_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV7855134.1"
                     /translation="HGIDPQYVRTIFTDQINATEGIEYARFGQWKFDSATAPTTAPDL
                     ADSRAAIDGFNKTMVDQIALQWNSLHSPTCAADVTAATGAVATDRRLDPLYRQALSSA
                     TRSYCPAT"
BASE COUNT          282 a          608 c          651 g          332 t
ORIGIN      
        1 ggcttcgcgg gcaccgcagt gatcatgctc gatggcgact gcgccttccg cgcctgttcg
       61 ccggacaccc gcatctggtg gggcgcctac ctgggtacac cggacgaact gctggtgtcg
      121 ggcaccgtcg gcgaggtcgg caagcagatc gtcgagatgc gggcggaggc ccgcgcccgg
      181 cacggctgga tcatggacac gtatctgctg cgaagcccgt agcggcgcta cccgagccgt
      241 tcgcgggcat ggcaggatcg gtcccatggt ggcattcctg ctgcgcgcgg cgctgaccgg
      301 attcgctttg tggatagttt ccctcgtcgt ttccggggtg gaattcgtcg gcggcgacac
      361 gaccctgcaa agggtcggga tcatcttggt cgtcgcggtg atcttcgggt tggtcaacgc
      421 gatcatcaag ccgatcgtgc agttcgtctc gattccgctg tacatcctca cgctcggcct
      481 gttccacatc gtggtcaacg cgctgatgct ctggatcacc tcgcggatca ccgagaacac
      541 cacgcactgg ggtctctaca tcgacgactt ctggtggacg gccatctggg cggccgtcgt
      601 gctgtcgatc gtcagctggg cgctgtcgct cgtcgtcagg gacactgcgg gcgtcccccg
      661 cggctgacgg gcacactgga ttcatgccag aactgcccga ggtcgaggcc ctggccgacc
      721 atctccgtcg gcacgccgtc ggcctcaccg tcgcacgcgt cgacgtgtcg gcgctgtcgg
      781 tactcaaaac gttcgacccg ccgccggccg ccctgcacgg ccaagaagtc gtcggcgcca
      841 accggtgggg caagtatctg ggcctgcagg cgggcgccct gcacctgatc acgcatctgt
      901 ccagggcggg ctggctgcgc tggtccgacc aactggccgc cgcaccgctc aagcccggca
      961 agggaccgat cgcactgcgc gttcacctcg gaacaccggg tgccgcagcg ggttttgacc
     1021 tcaccgaagc cggcacgcag aagcggcttg cggtctggtt ggtcgacgac ccgcagaagg
     1081 tacccggcat cgcggccctg ggcccggatg cgctggggct gaccgccgac ggtctcgccg
     1141 aggtactcgc cgggcagagc ggccgcatca agaccgtcat caccgaccag aaggtgatcg
     1201 cgggcatcgg caacgcctac agcgacgaga tcctgcacgt ggcgaagctg tcgccgttcg
     1261 cgacggcggg caagctcacc gatgcgcagt tggcgtcgct gcacgacgcg atgacctcgg
     1321 tgctcaccga cgcggtgacg cggtcggtgg gtcagcaggc ggcgaccctg aagggggaga
     1381 agcgttctgg cctgcgggtg cacgcccgca ccggcatgcc gtgcccggtg tgcggcgata
     1441 ccgtgcgcga agtgtccttc gccgacaagt cctttcagta ctgcccgacc tgtcagaccg
     1501 gcgggaaggt gctcgccgac cgtcggctgt cgcggctgct caagtagccg ggcagtagga
     1561 gcgggtcgcc gacgacaacg cctggcggta cagcgggtcg agtcgacggt cggtggcgac
     1621 ggcgcctgtc gccgcagtga cgtctgcggc gcatgtcgga ctgtgcagtg aattccattg
     1681 cagcgcaatt tgatctacca ttgtcttgtt gaacccgtcg atcgccgcgc gggagtcggc
     1741 caagtcgggg gcggtggtgg gcgcggtcgc cgaatcgaac ttccactgac cgaagcgcgc
     1801 gtattcgatg ccctcggtgg cgttgatctg gtcggtgaag atcgttcgga cgtactgcgg
     1861 gtcgatgccg tgg
//
LOCUS       DASYLX010001176         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_869581,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001176 DASYLX010000000
VERSION     DASYLX010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869581"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGP27_27800"
     CDS             complement(<1..360)
                     /locus_tag="VGP27_27800"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV7855135.1"
                     /translation="MPNMRRLHGRRIAVLAADGFEKVELTIPMRALQMTGAHVDVISL
                     RHGRIRGVNLHMPATRVGVDKTISEADPADYDGLLLPGGFINPDLLRQSAEARQFVRE
                     FANSDKPIATLCHGPWVL"
     gene            687..1592
                     /locus_tag="VGP27_27805"
     CDS             687..1592
                     /locus_tag="VGP27_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7855136.1"
                     /translation="MIASIKKAARLAGMRPIPRWPVRTAVNLRGKRILLTGASSGIGA
                     VAAQRLAAEGATVVAVARRKTLLTEVVERIVACGGSATAIPVDLSDLDQIDELVDLVG
                     PIDILINNAARSIRRPLAESLERWHDIERVMALNYYAPLRLIRGLAPGMIERGDGHII
                     NIATWRVLPESSPMFAAYNASKAALSAVGRVIETEWGHLGVHSTTIYYPLVATPMIAP
                     TRAYDGLPALTAEEAADWMVTAAKTRPIRIAPRKALALRALDVVAPRMLNNVLERETN
                     RMNAKFHQPEVANPSVEATIPVALP"
     gene            1687..2661
                     /locus_tag="VGP27_27810"
     CDS             1687..2661
                     /locus_tag="VGP27_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7855137.1"
                     /translation="MMIKSAIGAMLAASLILTGCADAGRTSDGHGYPSGPVTMTAGAN
                     PGSGFDLTIRSVVEALQKEKLVDVPLPVQNRPGASGAHFLATMVERYKGVDDQVSVTS
                     LSMMMNQLRGKSKYGYDDVTMIARLMTEYFVVVTGPGSPFKNLGDVMTAIKSDPAHVA
                     VGAAHDDEAPFDLLVSAAGGDPSSVRYITHEGGGDQSAALLKGDIGVAIGGVSEFITL
                     VKSGGLAALGVLAEKRLPGVDAPTAREQGLDVTLSNWRGLYGPPDMPKFAVAYWQKAL
                     GEMVKSPTWNQIAERSQFTTTFMIGDEFQTFLAKTQADVKAALEEAGQ"
     gene            2658..3593
                     /locus_tag="VGP27_27815"
     CDS             2658..3593
                     /locus_tag="VGP27_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7855138.1"
                     /translation="MTDGETSGIGVGRRPENLLLGAALTTAAFFCVALVGTLAKVSGQ
                     YTSTGVLLLFQNLICLVFVMPVALRGGWSSQRTTKLGLHLLRAATGTACWYALFFAIT
                     QIPLANATLLTYSAPLWMPLVAWIATRQRVSRWTWLGAGLGFAGVLLVLQPQSHSFNI
                     GEVSALAGALFLAVAMMSVRWLGATEPITRVLFYYFLLSTVMSVPIAVLDWKPFPAQA
                     LGWLIGLGLAQMLSQILIVVAYRYASAEKVGPFIYSVIVFTALIDWIIWDHPPTLFMY
                     LGMALVIGGGIVAVRARTGAPMAEVASAPREAPAR"
BASE COUNT          631 a         1192 c         1129 g          674 t
ORIGIN      
        1 cagcacccac gggccgtggc acagcgtggc aatcggcttg tcactgttgg cgaattcgcg
       61 cacgaactgc cgtgcctccg cggactgccg cagcaggtcg gggttgatga atccgcccgg
      121 cagcagcaga ccgtcgtagt cagccgggtc ggcctcgctg atcgtcttgt ccacaccgac
      181 ccgcgtggca ggcatgtgca ggttcactcc gcgtatccgg ccgtgccgca gggaaatgac
      241 atccacatgc gcgcctgtca tttgcaacgc gcgcatcggt atcgtcaact cgaccttctc
      301 gaagccgtcg gccgccagga cggcgattct tcggccgtgt agccgtcgca tgttgggcat
      361 gggttcctcc cgttgttagg tagcgcctca agcgggctgg ctggctgctc acccactacg
      421 gccataccct cgaccgaccc gtccacacct gcttccgaac cggttgttcg ggagctccca
      481 gttggttttt cgcggggagt gacgccacga gggagggccg tcgggtgtca acgcgccgat
      541 gccttgccga cgaactcgac accaacctca tgagctgttc atcacaatct ttctgacgaa
      601 tacgcaaatt tgctccgggc gtgagcgaag caccatagat tattagcgga cgcctacgtg
      661 tgccgaccgg agcaggggtg cagtttgtga tcgcgagcat caaaaaggct gcacgactgg
      721 ctggaatgcg gccaataccg cgatggccgg tccgcacggc ggtgaatctg cggggtaagc
      781 ggatcttgct gaccggtgcg tcctcgggaa tcggtgcggt tgccgcgcag agactcgccg
      841 ccgaaggcgc gacggtcgtc gcggtggcac gtcgcaagac ccttctgacg gaggttgtcg
      901 agcggatcgt cgcctgcggc ggcagtgcaa ccgccatacc ggtggacctg tccgacctcg
      961 atcagatcga cgagttggtc gatcttgttg gcccgataga cattctgatc aacaacgccg
     1021 ccagatccat ccgcaggccg ctggccgagt cgctggaacg ctggcacgac atcgagcggg
     1081 tcatggcgct gaactactac gcaccactgc gactcatcag gggcctggcc cccggaatga
     1141 tcgaacgcgg cgacggacac atcatcaaca tcgccacctg gcgcgtgctg ccggagtcgt
     1201 cgccaatgtt cgccgcttac aacgcctcca aggccgcgct gagcgccgtg ggccgagtca
     1261 tcgagaccga gtggggccat ctcggggtgc attccaccac catctactac ccgttagtgg
     1321 cgacgccgat gatcgccccc acccgcgctt acgatggctt gcctgcgctc accgccgagg
     1381 aggccgccga ctggatggtg accgccgcca agaccagacc gatccgcatc gcaccccgca
     1441 aggcacttgc gctacgagcg ctggatgtcg tcgcgccccg aatgctcaac aatgtcttgg
     1501 agcgcgagac caaccgaatg aatgccaagt tccaccagcc cgaagtcgcc aacccgtcgg
     1561 tggaagcgac cattccggtt gctcttccct gacgcatgtc catagtgacg tgcagacgcg
     1621 cccgatcgcc gtggatcgca ggcccgggaa cgtcgccgac ctatacgaac caggagcgca
     1681 cagcccatga tgatcaaatc cgcgatcggc gccatgctgg ctgcttcgct catcctgacg
     1741 ggttgcgcgg acgccgggcg aacctccgac ggacacggct atccgtcggg ccctgtaacg
     1801 atgacggcgg gagccaaccc cgggagtgga ttcgacctca cgatccgttc ggtcgtcgaa
     1861 gcattgcaga aggagaagct cgtcgacgtt ccgctgccgg tgcagaatcg gccgggtgcc
     1921 agcggcgccc actttctggc caccatggtt gagcggtaca agggcgtaga cgatcaggtt
     1981 tcggtcacgt ccctgtcgat gatgatgaac cagcttcgcg gtaagtcgaa gtacgggtac
     2041 gacgacgtca ccatgatcgc ccgcctgatg accgagtatt tcgtcgtcgt caccggcccc
     2101 ggttccccgt tcaagaacct cggtgacgtc atgacggcaa tcaagtcgga tcccgcgcac
     2161 gtcgcggtcg gtgccgccca cgacgacgag gcccccttcg acctgctggt gtccgcggca
     2221 ggcggggacc cgtcatctgt caggtacatc acgcatgaag gaggcggtga ccagagcgct
     2281 gcgcttctca agggcgacat cggcgtggcc attggcgggg tcagcgagtt catcaccctg
     2341 gtgaaatcgg gaggccttgc ggcgcttggt gtgctggccg agaaacggtt gcccggtgtc
     2401 gacgctccga ccgcccggga gcagggcctc gacgtcaccc tctccaactg gcgaggcctc
     2461 tacggccccc cggacatgcc gaagttcgca gttgcgtact ggcagaaggc gctcggcgaa
     2521 atggtgaagt cacccacctg gaaccagatc gcggagcgga gtcagttcac caccaccttc
     2581 atgatcggtg acgagtttca gaccttcttg gcgaagaccc aggcggacgt caaagccgcc
     2641 ctcgaggaag ccggccagtg actgacggcg agacgtccgg tatcggcgtg ggccgcagac
     2701 ccgagaacct ccttttggga gcagccctga ccaccgcggc gttcttctgc gtcgcgctgg
     2761 tgggcaccct cgccaaggtg tccggtcagt acacctcgac cggagtgctt ctgctcttcc
     2821 agaacctgat ctgcctggtc ttcgtgatgc cggtggcgtt gcggggcggc tggtcgtcac
     2881 aacggaccac caagctgggg cttcacctcc tgcgggcggc aacgggtacc gcgtgctggt
     2941 atgcgttgtt cttcgcgatc acacagatac cactcgccaa cgcgacactt ctgacctaca
     3001 gcgccccctt gtggatgcca ctcgtcgcct ggatcgcaac ccgtcaacgg gtatcgcggt
     3061 ggacctggct cggcgccggt ctcgggttcg ccggagtact actcgtgtta cagcctcaga
     3121 gccatagctt caacatcggc gaggtgtccg cgctcgcagg cgcgctgttc ctcgccgtcg
     3181 cgatgatgtc cgtacggtgg ctgggcgcga ccgagcccat cacccgcgtt ctcttctatt
     3241 acttcctgct ctcgaccgtg atgtccgttc cgatcgcagt actggactgg aagcccttcc
     3301 ctgcgcaggc cctggggtgg ttgatcggac ttggtctggc gcagatgctt tcgcagatcc
     3361 tcatcgtcgt ggcctaccgc tacgcatcgg cggagaaggt tggcccgttc atttactccg
     3421 tcatcgtgtt cacggcgctc atcgactgga tcatctggga ccatccgccg acactgttca
     3481 tgtatctcgg catggcgctg gtcatcggtg gcggcatcgt ggccgttcgc gccagaaccg
     3541 gcgcgccgat ggcggaagtc gctagcgcgc ctcgcgaggc acccgcacgg tgaccatcat
     3601 cgccgccagg cccgctgcga gcacca
//
LOCUS       DASYLX010001177         6451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2735015,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001177 DASYLX010000000
VERSION     DASYLX010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735015"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGP27_27820"
     CDS             <1..183
                     /locus_tag="VGP27_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV7855139.1"
                     /translation="AAASYAVWQVDELEVSAPDNAVQRWSTDPRSRVPALPRLGVDEP
                     LPRCRQTVHRGAVIHG"
     gene            176..2008
                     /gene="lnt"
                     /locus_tag="VGP27_27825"
     CDS             176..2008
                     /gene="lnt"
                     /locus_tag="VGP27_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV7855140.1"
                     /translation="MVKRTPHRRRGRAEDDTDSFPVFDYERADEVDGDGEATSADTTA
                     DEPAAKREVPAWVQRFGAAVVARLSHISAALVGGLLLCLSFPPFGWWYTAIVAFALLA
                     WVLTRDTTKLVGGFGYGFLFGAAFYIPLLPWTGAMVGLLPWLVLSLVQAVFPALFGLG
                     AVAVRRLTAWPIWFAGLWALAEWAKSTVPFGGFPWGVVGFGQTDSPLLSLAQIGGVPL
                     VSFAVTLLGFALAALAFEIFDWWRGGGEGKPADSAPPSVVLPGACICLVLIATAAISP
                     AVRHSGAGAADEPSVNVAAVQGNVPQLGLDFNAQRRAVLDNHVRETMRLADDVRAGRA
                     PQPLFVIWPENSSDIDPLANQDAADQMGVAVEAIKAPILVGGVLKGAGYTPDNPVSTN
                     SIIVWNPGTGPGDRHDKQIVQPFGEYLPWRSFFKHFSPYAERAGYFAAGSGNGVVEAA
                     GVPIGVSTCWEVIFDRSPRESVLSGAQVLTVPSNNATFNESMSVQQLAFARLRAVEHN
                     RFVIVAGTTGISAVVAPDGRVLARTGWFEPAYLDMAIRLKTQLTPATRFGPLVQGLLV
                     AVGIGALIAAILHNERFVRSRRRAKNAGGQDTPPDDGGTERGTT"
     gene            2005..2772
                     /locus_tag="VGP27_27830"
     CDS             2005..2772
                     /locus_tag="VGP27_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEV7855141.1"
                     /translation="MSGPGPAERPSQRTLIIIPTYNERENLPLIAGRVHTALPDVHIL
                     VVDDGSPDGTGELADELALADPDRVHVMHRKSKNGLGAAYLAGFAWGLSREYSVLVEM
                     DADGSHAPEELHRLLDAIDGGADLAIGSRYVPGGAIRNWPRRRFALSKTANTYARVLL
                     GVGVRDITAGYRAYRREVLEKIDLAAVDSKGYCFQIDLTWRAINNGFVVVEVPITFSE
                     RELGVSKMSGSNIREAMLKVAEWGVRGRLDRARGVMR"
     gene            complement(2780..3121)
                     /locus_tag="VGP27_27835"
     CDS             complement(2780..3121)
                     /locus_tag="VGP27_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEV7855142.1"
                     /translation="MADRVLRGSRLGAVSYETDRNHDLAPRQVARYRTDNGEEFDVPF
                     ADDAEIPGAWLCRNGMEGTLIEGDVPEPKKVKPPRTHWDMLLERRSVDELEELLKERL
                     DIIKTRRRGSS"
     gene            3252..3584
                     /locus_tag="VGP27_27840"
     CDS             3252..3584
                     /locus_tag="VGP27_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855143.1"
                     /translation="MTRTRRRPASCRWCGREVADAGLGRRRQYCRQSCRQRAYEQRAQ
                     VRGTSLAPDSVVLTAEEASELSDRVYQVRCAAEDVSTAIEEGAHPTELRELCDALMRA
                     ARAADGWR"
     gene            3597..4790
                     /locus_tag="VGP27_27845"
     CDS             3597..4790
                     /locus_tag="VGP27_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7855144.1"
                     /translation="MWRFDRFVLDTQRYELRHGDEVVRVEPQVFDVLTQLVINRERCV
                     TKEELFDAVWGGRFVGEAALTSRIKAARRALGDDGESQRFIRTVRGRGYQFVATVLDD
                     TPPTPQEPEAQDEPPPPRQHIAFCRGADGVRLAYAVAGEGPPLVRAANWMTHLGYDIE
                     SPVWRHWVRDLSRRHTFIRYDERGCGLSDWSATDFSFGDWVSDLESVVEALGLERFPL
                     LGVSQGGAVAVAYAARHPDRVSKLVLCSSYARGRGTRAVNEEEKRAAALDLDLARVGW
                     GRDDPAFRQVFAAQFLPDGTRADWAAFDQLQRRTTSPENAVRFLEEFGRIDVRDEAGQ
                     VSCPTLILHSRDDHRQPLRVAEELAALIPDSHLVALNSNNHLLTANEPGWQVFLDEVE
                     RFLAE"
     gene            4884..5609
                     /locus_tag="VGP27_27850"
     CDS             4884..5609
                     /locus_tag="VGP27_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855145.1"
                     /translation="MKKHLRILLLGVIALLALGSLSACSSGTKEATESTEASKTSATS
                     ATPAPATAAPFPQSAKYIADVKAADGKPMVIGIAVDGTQIAAYACNGTDDEAWFFGDQ
                     KDGKIDITSRLKDTLTAEFDGKDVVGDLTLNGVAFKFSAPAVPAPAGMYTADFDGVRA
                     SWVVRPDGTATGVQFNGGISGRDFEQAELQQLNEQQFRNSVRNKRQLQQAQQITLLAN
                     KSARSTINGHEVTPVIVDGDFRL"
     gene            5744..>6451
                     /locus_tag="VGP27_27855"
     CDS             5744..>6451
                     /locus_tag="VGP27_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7855146.1"
                     /translation="MTTPTELAPARTAPARRSDRRWTVIRWASPVALLALWQLGSAIG
                     LIAEDVLPAPSLILEAGVEVVENGQLGDALRVSGIRVVEGLLLGGLLGVALGIAVGLS
                     RWFEAAVDPPMQMVRALPHLGLIPLFILWFGIGELPKVLLVALGVSVPLYLNTFSAIR
                     QVDPKLFETAAVLGFSFWQRLRIIIVPSAAPQVLVGLRQSLAIAWLTLIVAEQINADK
                     GIGFMINNARDFLRIDII"
BASE COUNT         1036 a         2142 c         2164 g         1109 t
ORIGIN      
        1 gcagccgcct cttacgcggt gtggcaggtc gacgagttag aggtcagcgc ccccgacaac
       61 gcagtgcaac gctggtccac cgacccgcgt tcgcgagtgc ccgcgctacc ccgtctcggc
      121 gtcgacgagc cgctgccacg atgccgtcag accgtgcacc gaggtgcggt gatccatggt
      181 taagcggacg ccacaccggc gacggggccg cgccgaggac gacaccgact cgttcccggt
      241 tttcgactac gaacgcgccg acgaggtcga cggcgacggc gaggcaacgt ccgcggacac
      301 gacagccgac gagccggccg cgaaacgcga ggtgcccgca tgggtccagc ggttcggcgc
      361 ggccgtggtg gcccggctct cgcacatcag cgccgcgctc gtcggcgggc tgctgctctg
      421 cttgagcttc ccgccgttcg gctggtggta caccgcgatt gtcgcgttcg cactgctggc
      481 ctgggtgctg accagggaca ccaccaagct cgtcggcggg ttcggctacg gcttcctttt
      541 cggggcggcc ttctacatac cgctgttgcc gtggaccggc gcgatggtcg ggctgctgcc
      601 atggctggtg ctatcgctcg tgcaggccgt gttccctgct ttgttcggcc tcggcgctgt
      661 cgcggtacga aggctgaccg cgtggccgat ctggttcgcc ggcctttggg cactcgccga
      721 gtgggcgaag tcgacggtgc cgttcggcgg attcccctgg ggcgtggtcg gtttcggcca
      781 gacagacagt ccgctgttgt cgctggcgca aatcggtggg gtgccgctgg tgtcgtttgc
      841 ggtgacgttg ctcggcttcg cgctcgccgc tctggccttc gagattttcg actggtggcg
      901 cggcggcggt gaaggcaagc ccgcggactc ggcgccgccc tcggtggtcc tgcccggcgc
      961 ctgcatctgt ctcgtgctga tcgccaccgc agcgatctcg ccagccgtca ggcattccgg
     1021 cgcgggtgcc gccgacgaac cgtcggtcaa cgtggcggcc gtgcagggca atgtgccgca
     1081 gctcgggctg gacttcaacg cgcagcgccg tgcggtgctc gacaaccacg tgcgggaaac
     1141 gatgaggctc gccgatgacg tgcgggcggg ccgtgcgccg cagccgttgt tcgtcatctg
     1201 gccggagaac tcgtcggaca tcgacccgtt ggccaaccag gacgccgccg accagatggg
     1261 ggttgccgtc gaggcgatca aggcgccgat ccttgtcggt ggagtgctca aaggtgcggg
     1321 ctacaccccg gacaacccgg tctcgaccaa ctcgatcatc gtgtggaatc ccggcaccgg
     1381 tcccggcgac cgacacgaca agcagatcgt ccagccgttc ggcgaatacc tgccatggcg
     1441 cagcttcttc aagcacttct cgccgtatgc cgagcgtgcg ggttacttcg cagccggcag
     1501 cgggaacggc gtcgtggagg ccgccggcgt gccgatcggg gtatcgacct gctgggaggt
     1561 catcttcgac cggtcgccac gggagtcggt gctcagcggc gcccaggtgt tgacggttcc
     1621 gtccaacaac gccacgttca acgaatcgat gagtgttcag cagcttgcgt tcgccaggct
     1681 gcgggcagtt gagcacaacc gcttcgtcat cgtggccggc actacgggca tcagcgccgt
     1741 cgtcgcgcca gacggccgtg tgctggcccg caccgggtgg ttcgaacccg cctatctcga
     1801 catggcgatc cggctgaaga cccagctcac accggcaacc cgattcggcc cgttggtcca
     1861 ggggctattg gtcgccgtcg ggatcggagc cctcatcgcc gcgatactgc acaatgagag
     1921 attcgtgcgc agccgccgcc gggcgaagaa cgccggcggt caagacacgc cgcccgatga
     1981 cgggggcacc gaaagaggaa ccacatgagc ggcccagggc cagcagaacg cccgagtcag
     2041 cgcactctaa tcatcattcc gacctacaac gagcgggaga acctgccgtt gatcgccggc
     2101 cgggtgcaca ccgccctgcc cgacgtgcac atcctcgtcg tcgacgacgg cagcccggac
     2161 ggcaccggcg agctggccga cgaattggcg ctcgccgacc ccgatcgggt ccacgtgatg
     2221 caccgcaaga gcaagaacgg gctcggcgcg gcctacctgg ccggcttcgc gtgggggctg
     2281 agccgcgagt attcagtcct ggtcgagatg gatgccgacg gcagccacgc gcccgaggaa
     2341 ctgcaccggc tgctcgacgc catcgacggc ggcgcggacc tggcgatcgg gtcgcgctac
     2401 gtgcctggcg gcgccatccg gaactggccg cgtcgccggt tcgcactgtc caagaccgcc
     2461 aacacgtacg ccagggtctt actcggcgtc ggcgtgcgcg acatcaccgc gggctaccgg
     2521 gcctatcgac gtgaggtgct cgaaaagatc gacctcgccg ccgtcgactc gaaggggtac
     2581 tgcttccaga tcgacctgac ttggcgcgcc atcaacaacg gattcgtcgt tgtcgaagtg
     2641 ccgatcacct tctccgagcg cgagctgggg gtatcgaaga tgagcggatc caacatccgc
     2701 gaagcgatgc tcaaggtcgc ggagtggggt gtccgcgggc gcctggatcg cgcccgcggc
     2761 gtgatgcgct agtcgctggt cagctgctgc cgcgacgccg ggtcttgatg atgtcgaggc
     2821 gctccttgag cagctcttcg agctcatcga cggaacggcg ctccagcagc atgtcccagt
     2881 gggtacgcgg cggcttgacc ttcttgggct ccggcacgtc accctcgatc aaggtgcctt
     2941 ccatgccgtt gcggcacagc caggcgccgg ggatttcggc gtcgtctgcg aacgggacgt
     3001 cgaactcctc gccgttatcg gtgcggtagc gggcgacctg acgcggcgcc aggtcgtggt
     3061 tgcggtcggt ctcgtagctc acggctccga ggcgactgcc tctcaacacc cgatcagcca
     3121 ttcgtcaaca ctcctcaaat caatcagtcc ggataaacaa cgcagcacac ttaccacgag
     3181 ttcccgactc agccgcaaat gataccggga tatgcgtctt tccccgtcta cagtcgggat
     3241 ccgtggagac tttgacccga acccgccgac gtcccgcatc atgccggtgg tgtggtcgcg
     3301 aagtggccga cgcgggtctc ggcaggcgcc gtcagtactg caggcagtcg tgtcggcagc
     3361 gcgcctacga gcagcgggcg caggtgaggg gcacgtcgct ggccccggat tcggtggtgc
     3421 tgaccgccga agaggcctcc gagctgtccg accgtgtcta tcaggtgaga tgcgctgcag
     3481 aggacgtttc gaccgcgatc gaagaaggcg cgcaccctac ggaactgcgt gaactgtgtg
     3541 acgctctgat gcgcgcggcc agggcggccg acggctggcg ataggcggga gccgccgtgt
     3601 ggcgtttcga ccgtttcgtg ctggacacgc agcgctacga gctgcgtcac ggtgacgaag
     3661 tcgtccgcgt cgagccgcag gtgttcgacg tcctgacgca actggtcatc aaccgcgaac
     3721 gctgcgtcac caaagaggag ttgttcgacg cggtgtgggg cggccgattc gtcggcgagg
     3781 cggcgttgac gagccggatc aaggcggctc gccgggcgct gggcgacgac ggcgaatcgc
     3841 agcgcttcat caggacggtg cgcggccgcg ggtaccaatt cgtcgccacg gtgctcgacg
     3901 acacaccgcc gactccgcag gagcctgagg cgcaggacga gccgccgcct ccgcgacagc
     3961 acatcgcgtt ctgtcgcggc gccgacgggg tgcggctggc ctatgcggtg gcgggggagg
     4021 gcccgccgct ggtgcgcgcc gcgaactgga tgacacatct cggctacgac atcgagagcc
     4081 cggtatggcg gcactgggtt cgcgatctct cgcgacggca cacctttatt cggtacgacg
     4141 aacgaggttg tggtctatca gactggagcg ccacggattt cagcttcggc gactgggtca
     4201 gcgatctgga gtccgttgtc gaggcactgg gactggagcg ctttccgctg ctgggtgtgt
     4261 cccagggcgg cgcggtcgcc gtcgcctacg ccgccaggca tcccgaccgg gtcagcaagc
     4321 tggtgctgtg cagctcctac gcccgtggcc gcggcacccg agcggtcaac gaggaggaga
     4381 agcgggcagc ggcactggac ctcgatctcg ctcgcgtggg ctggggccgc gacgacccgg
     4441 ccttccggca ggtgttcgcc gcacagttcc tgcccgacgg caccagggcc gactgggcgg
     4501 ccttcgacca gttgcagcgg cgcaccacct cgccggagaa cgcggtgcgg ttcctcgagg
     4561 agttcgggcg catcgatgtg cgcgacgagg ccggtcaggt ttcgtgcccg acattgatcc
     4621 tgcactcgcg cgacgatcac cggcagccgc tgcgcgtcgc cgaggaactc gccgcgctga
     4681 tccccgattc tcatctggtc gccctcaaca gcaacaacca tctgttgact gcaaacgaac
     4741 cgggatggca ggtgtttctc gacgaagtgg agcgcttcct ggcggagtga gccgggcacg
     4801 gttgcctgcg ggcgcctcgg atctccacgc aatctccatg cattcttcat gtgcggtcgc
     4861 tgtgagcgaa tcatcctggt cacatgaaaa aacatctgcg catcctgcta ctgggtgtca
     4921 tcgcgcttct cgcgctcggc tccctgtccg cctgctcgag cggcacgaaa gaagccaccg
     4981 agtccactga agcctccaag acctccgcaa cttccgccac ccccgctccc gccaccgcag
     5041 ccccgttccc gcagtcggcg aagtacattg ccgacgtcaa ggcggccgac ggcaagccaa
     5101 tggtcatcgg catagccgtc gatggcaccc agatcgccgc atacgcgtgc aacggcaccg
     5161 acgacgaggc ctggttcttc ggcgatcaga aggacggcaa gatcgacatc acgtcccgcc
     5221 tcaaggacac cctgacggcc gagttcgacg gcaaggacgt ggtcggcgac ctgaccttga
     5281 acggcgtggc cttcaagttc agcgcacccg ccgttccggc gcctgcaggc atgtacaccg
     5341 ccgacttcga cggtgtgcgc gcgtcgtggg tggtccgtcc cgacggcact gcgaccggcg
     5401 tgcagttcaa cggcggaatc agcggccgcg acttcgagca ggccgagttg cagcagctga
     5461 acgagcagca gttccgcaac agcgtgcgta acaagcgtca gctgcagcag gcccagcaga
     5521 tcacgctgct ggcgaacaag tcggccaggt cgaccatcaa cggccacgaa gtgacgccgg
     5581 tgatcgtcga cggtgacttc cggctgtgac cacaaccaca tagcgcgcag gcaacggccg
     5641 tgccggagta agtgtttcga cacctactcc ggcagggtcg tttatttcac gcagaattca
     5701 aaccttcacc ctcgcctcgg gtgcgccgta gcgtcaaagc accgtgacga caccaaccga
     5761 gctggcgcct gcccgcaccg ccccggcgcg ccgaagcgac cgcagatgga ccgtcatccg
     5821 atgggcctcg ccggtcgcct tgcttgcgct gtggcagttg ggaagcgcga tcggactcat
     5881 cgcagaggat gtgctgcccg cgccgtcgct gatcctcgag gccggcgtcg aagtcgtcga
     5941 gaacggacaa ctcggcgacg ccctgcgggt ctcgggcatt cgggtggtcg aagggctgct
     6001 gctcggcggg ctcctcgggg tcgccctcgg tatcgcggtc ggcctttcgc ggtggttcga
     6061 ggcggccgtg gacccaccga tgcagatggt gcgtgcgctg ccgcacctcg gcctgattcc
     6121 gctctttatc ctgtggttcg gcatcggcga gcttccgaag gtgctcctgg tagcactggg
     6181 tgtgagcgtc cctctctatc tgaacacctt ctcggcgatt cgtcaggtcg acccgaagct
     6241 ctttgaaacc gctgcggtgc ttggcttctc gttctggcag cggctccgga tcatcatcgt
     6301 ccccagcgcg gcgccccagg tgctcgtcgg tttgcggcag tcgctcgcga ttgcatggct
     6361 gacgttgatc gtcgccgagc agatcaacgc ggacaagggc atcggcttca tgatcaacaa
     6421 cgcgcgggac ttcctgcgca tcgacatcat c
//
LOCUS       DASYLX010001178         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3394163,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001178 DASYLX010000000
VERSION     DASYLX010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3394163"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGP27_27860"
     CDS             <1..232
                     /locus_tag="VGP27_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7855147.1"
                     /translation="IDRGIASGEFYECDSHVLALGYIGMTLGSYRWLRPQGRRTAQEI
                     AAEFSTALLRGLIRDEAVRVESPLGLDVGEHA"
     gene            262..702
                     /locus_tag="VGP27_27865"
     CDS             262..702
                     /locus_tag="VGP27_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7855148.1"
                     /translation="MPLVSKTVEVDASAEAIMSIVADFEAYPQWNEEIKGCWVLARYD
                     DGRPSQLRLDVVVQGQSGTFITAVYYPGPNEIYTVLQQGDHFDKQEQKFSVVPIGPTS
                     LLTVDLEVETKLPIPKTMVKKAIGDTLDYLAGNLKARAEQLAAS"
     gene            complement(695..1039)
                     /locus_tag="VGP27_27870"
     CDS             complement(695..1039)
                     /locus_tag="VGP27_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855149.1"
                     /translation="MRRLRGYELRCVLTLHLFQHGPATLADLLDMLDFHGFEVAGRAS
                     RTVSDALRCEMAHERVRRLRRGRYGPASMPRATEYRIHQRALALRAEAAALNAESNEA
                     FWDALFGKREAS"
     gene            complement(1224..>1755)
                     /locus_tag="VGP27_27875"
     CDS             complement(1224..>1755)
                     /locus_tag="VGP27_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7855150.1"
                     /translation="SITPVREALRTLRGDGMVLLEPHRGHVVAPLSRRDIEDIFWLQA
                     TIAKELAAAATERIADDEIADLERLNDELATTVATGEPQAIAAAEFAFHRSLNHTTGR
                     IKLAWFLLHVARYMPPLIYAADPQWGVEAVENHRGLIAALRRRDVAEVVRLTGWQFDD
                     GARRLTGRLEKTGIWS"
BASE COUNT          299 a          567 c          591 g          298 t
ORIGIN      
        1 catcgaccgc ggcatcgcca gcggcgagtt ctacgaatgc gactcgcatg tgctggcgct
       61 cggctacatc ggcatgaccc tcggctcgta tcggtggctg cgcccgcagg gcaggcgcac
      121 cgcccaggag atcgcggccg aattcagcac cgcgctgctg cgaggcctga tccgtgacga
      181 ggccgtccga gtggagtcgc cgctgggcct cgacgtcggc gagcacgcct agtgcagatc
      241 cggtgccgat aggctgcctc aatgccgttg gtgagtaaga cagtggaggt cgacgcgtcc
      301 gcggaggcga tcatgtcgat cgtcgcggac ttcgaggcgt acccgcagtg gaacgaagag
      361 atcaagggct gctgggtgtt ggcacgctac gacgatggac ggcccagcca gctccggctc
      421 gatgtggtcg tccaggggca gtcgggcacc ttcatcacgg cggtctacta cccgggcccg
      481 aacgagatct acacggtgct gcagcagggt gaccatttcg acaagcagga gcagaagttc
      541 tcggtcgtcc cgatcggccc gacgtcgctg ctgaccgtcg acctcgaggt ggagaccaag
      601 ctcccgatcc cgaagacgat ggtgaagaag gcgatcgggg acaccctcga ctatctcgcg
      661 ggcaatttga aagcgcgggc cgagcaactc gctgctagct agcctcgcgc ttgccgaaca
      721 gcgcatccca gaaggcctcg ttcgattcgg cgttcagcgc cgccgcttcc gcccgcagcg
      781 ccagtgcccg ctgatggatt ctgtattcgg ttgctcgtgg catggaggcc ggcccatatc
      841 ggccgcgccg caaccgtcgc accctttcgt gtgccatctc gcaacgcagc gcgtccgaaa
      901 ccgtccttga agcgcggccg gcgacctcga agccatggaa gtcgagcatg tcgagcagat
      961 cggccaacgt cgcagggccg tgctggaaca gatgaagggt caaaacgcac cgcagttcat
     1021 agccgcggag ccggcgcatc tttctcatga ccgaacactg tcagcgatgt ctgacatcgg
     1081 atcggcggcg ggtgagcgga aagtcggcgt cgcggcactg cccgcctccg gtcgacattg
     1141 tcggcgacag ttagcaggtc tttcttcggc cgacagtgtc gaccaaagga gggcacgtcg
     1201 gaaagtggtt ctccgacgga acgtcagctc cagatgcccg ttttctcgag tcgcccggtc
     1261 aggcgtctcg cgccgtcgtc gaattgccag ccggtcaggc gaaccacctc ggcgacgtcg
     1321 cggcggcgca gcgccgcgat cagcccgcga tggttctcca ccgcttcgac gccccactgt
     1381 gggtcggcgg catagatcag cggcggcatg tagcgcgcca cgtgcagcag aaaccacgcc
     1441 agtttgatgc ggccggtcgt gtggttcagc gacctgtgga acgcgaactc agcggcggcg
     1501 atggcctggg gctcaccggt cgcgaccgtc gtggcgagtt cgtcgttgag ccgctccaga
     1561 tcggcgatct cgtcgtcggc gatccgctcg gtggcggccg ccgcgagctc cttggcgatc
     1621 gtggcctgca gccagaagat gtcctcgata tcgcgcctgc tcaagggggc cacgacgtgg
     1681 ccgcggtggg gctccagcag caccatgccg tcaccacgca gggtgcgtag cgcttcgcgc
     1741 accggggtga tgctg
//
LOCUS       DASYLX010001179         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2844452,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001179 DASYLX010000000
VERSION     DASYLX010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844452"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGP27_27880"
     CDS             complement(<1..310)
                     /locus_tag="VGP27_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEV7855151.1"
                     /translation="MTAPRYVLTRRPGMQLDVFATRCGLHPDMVRRLVALGLVASRPD
                     AHGDLWFEPSAVVTVARIQRLRTGLGLNYAAIGLVLDLLDRIEELESASRRRRTPRWT
                     S"
     gene            complement(307..1227)
                     /locus_tag="VGP27_27885"
     CDS             complement(307..1227)
                     /locus_tag="VGP27_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV7855152.1"
                     /translation="MARDYYEVLGVSRDATADQIQQAFRTLARKYHPDVNKDPSAEDR
                     FKEINDAYHVLSDPETRKRYDRFGEDFRQVPEDWEERVGAGAGGFGGGRPAGGRVRYG
                     QGFGGGGGGGINIEDLFGDMFGGSGGFGPIPGADQEAVLELTVEEAYRGGKKQVSLDG
                     RTYDVNIPPGVIDGQRIRLAGEGGRGSGNGPAGDLYLVVRIKPHPRFRLSGRDVTVDL
                     PVSPWEAVLGTTVAIRTPGGEAKVKVPKGSSTGRRLRLRGEGMPNPRGAPGDLYAEIK
                     VMVPPKPTARERELFEQLAAVSTFDPRRGR"
     gene            complement(1234..1758)
                     /locus_tag="VGP27_27890"
     CDS             complement(1234..1758)
                     /locus_tag="VGP27_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005180075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV7855153.1"
                     /translation="MVSPAEHSKTDEGDSREGVDPPETAVSQSDSDKELAKLEDRWRR
                     AVADLDNLRKRHARELDRERATERFGVASAWLPIVDNLERAISHAGDQSDAVVDGVRT
                     ILGQAQQLLEQLGYRRDAEAGVPFDPVRHEVVGVVDQPDSEPGTVVEVLRPGYGEGAT
                     QLRPAAVVVSRHGE"
     gene            complement(1764..>2297)
                     /locus_tag="VGP27_27895"
     CDS             complement(1764..>2297)
                     /locus_tag="VGP27_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7855154.1"
                     /translation="FKLENIRPAPRGEPQIEVIFDIDANGILHVTARDKDTGAEQGIT
                     ISETSNLDKSEVERMLAEAEAHRREDEELRQLVEARNALDSAAYQVERRLAELGDSAA
                     PHERARAEMLIADARRAIEEEAPMDRVQALTSELQQVLYGLQPAPSGDGNGQPTGDGQ
                     GSTVGDDDVVDAEFDRG"
BASE COUNT          320 a          815 c          731 g          431 t
ORIGIN      
        1 ttgatgtcca ccgcggtgtc ctccttcggc gagaagcgga ttccagctct tcgatgcggt
       61 ccagcaggtc gagcaccagc ccgatcgccg cgtagttcag tcccagaccg gtcctcaacc
      121 gctggatgcg ggcgactgtc accaccgcgg acggttcgaa ccagagatcg ccgtgggcat
      181 ccggccggga agcgacgaga ccgagtgcca ccagccggcg caccatgtcc ggatgcaacc
      241 cacaacgtgt cgcgaagacg tcgagctgca ttccgggccg ccgcgtcagg acatagcgtg
      301 gtgcggtcat cgtcccctcc tggggtcgaa tgtggacacc gccgccagtt gctcgaacag
      361 ctcacgttct cgcgcagtgg gtttcggtgg caccatcacc ttgatttctg cgtagaggtc
      421 gcctggggca ccgcgcgggt tgggcatgcc ttcgccgcgc agccgcaacc gacggccggt
      481 tgaggaaccc ttcggcacct tgactttcgc ctcgccgccg ggggttcgga tagcgaccgt
      541 cgtgcccagc accgcctccc acggcgacac cggcaggtcg actgtgacgt cgcgtccgga
      601 aagccggaat cgggggtgcg gcttgatccg caccactagg tagagatctc cggcaggtcc
      661 attgccgctc ccgcggccgc cctctccggc cagccgtatc cgctgaccgt cgatgacgcc
      721 ggggggaatg ttgacgtcgt aggtgcggcc gtccagtgag acctgctttt tgccgccccg
      781 gtaggcctcc tcgacggtca gctcgagcac cgcttcctgg tcggcgcccg gaatcggccc
      841 gaatccgcca ctgccgccga acatgtcgcc gaaaaggtct tcgatgttga tgccgccgcc
      901 gcccccgccg ccgaagccct ggccgtagcg cacccggccg ccggcaggcc gtccgccacc
      961 gaagccaccc gcaccggcgc ccacccgctc ttcccagtcc tcgggaacct gtcggaagtc
     1021 ttcgccgaaa cggtcgtagc gcttgcgggt ttcggggtcc gagagcacgt ggtaggcgtc
     1081 gttgatttcc ttgaaccggt cttcggccga cggatccttg ttgacgtcgg ggtgatactt
     1141 gcgggcaagt gtgcggaacg cctgctggat ctgatcggcg gtcgcgtccc gcgacacccc
     1201 gagcacttcg tagtaatcgc gggccacgga gggtcactct ccgtgtcggc tgaccaccac
     1261 ggccgccggc cgcagttgtg tggcaccttc gccgtagccc ggtcgaagca cttcgacaac
     1321 cgtcccgggc tcgctgtccg gctgatccac cacaccgacc acctcgtggc gcaccgggtc
     1381 gaagggcacc cctgcctcgg catcgcggcg atagcccaat tgctccagaa gctgttgcgc
     1441 ttgtccgaga atggtccgca caccatcgac gaccgcgtcg gactggtcgc cggcatgact
     1501 gatggcccgc tccaggttgt ccacgatcgg cagccacgcg ctggccacgc cgaatcgttc
     1561 cgttgctcgt tcgcggtcca gttcgcgggc atgccgcttg cgcagattgt ccagatcggc
     1621 gacggcgcgc cgccagcggt cctcgagctt ggccagctcc ttgtccgaat cggattgcga
     1681 gacagccgtt tctggcgggt ctacgccttc gcggctgtcg ccctcgtcgg tcttggaatg
     1741 ctctgcgggg ctgaccatgc ggctcagccc cgatcgaact ctgcgtcgac cacgtcgtcg
     1801 tcaccgaccg ttgagccctg tccgtcgccg gtcggttgcc cgttgccgtc acccgacggc
     1861 gcgggttgca gcccgtaaag cacttgctgc agttcggacg tcagggcctg cacccggtcc
     1921 atcggcgctt cttcctcgat cgctcggcgc gcgtcggcga tcagcatctc ggcacgtgcc
     1981 cgctcgtgtg gcgccgccga gtcgcccagt tcggcaagcc gccgttccac ctggtatgcc
     2041 gccgagtcga gcgcgttgcg cgcctcgacg agctggcgca gctcttcgtc ctcgcggcgg
     2101 tgcgcctcgg cctcggccag catccgctcc acctcgctct tatcgaggtt ggacgtctcg
     2161 ctgatggtga tgccctgctc ggcgccggtg tccttgtcgc gcgctgtgac atgcaggatg
     2221 ccgttggcat cgatgtcgaa gatgacctcg atctgcggtt caccccttgg tgccgggcgg
     2281 atgttctcca gcttgaa
//
LOCUS       DASYLX010001180         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3721692,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001180 DASYLX010000000
VERSION     DASYLX010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721692"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..957
                     /locus_tag="VGP27_27900"
     CDS             40..957
                     /locus_tag="VGP27_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016692068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7855155.1"
                     /translation="MTQVAQALEVRPQSGWTGAEIHGIDLTKPLDDQQVAEVRAALLQ
                     WKVVFFRDQFIDHDDHLRFASAFGKPTPAHPLFDSIPDPNYPTIYPIFRDRFKARYGS
                     SRGFDKPHWHADVTAAVNPPAASILRAEVIPPYGGDTQWSNTVAAYAGLSAPLQRLAD
                     KLRAVHRFSPPDGANATEDYQRRVNRRPLVTEHPVVRVHPETGEKALYVNPGFTTHIV
                     GVSPHESRRLLGLFYDELVRPEYTVRFKWEPGSIAFWDNRSTVHLAPSDLTIDHDRRL
                     YRVTLVGDVPTGPDGRQSVALDGEPFEAI"
     gene            complement(1143..1889)
                     /locus_tag="VGP27_27905"
     CDS             complement(1143..1889)
                     /locus_tag="VGP27_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7855156.1"
                     /translation="MSASLEGKVAFVAGASRGIGATVAAALAREGAAVAVAARSEHEG
                     KLPGTIHTVAETISSSGGRALAVTCDVTKEDSVQAAVAATVAEFGGIDILVANAGVLW
                     LGPIEATPLKRWQLCLDVNLTGVFLATKAVIPNVRARGGGSLVAVTTTGVTMTDQGAN
                     AYWVSKAGVERLYLGLAADLKPDNIAVNCLSPSRVVLTEGWHAGGAGMEIPPELVEPP
                     EAMANAAVLLAQQDAGGITGTIQRSESLTF"
     gene            complement(1886..2650)
                     /locus_tag="VGP27_27910"
     CDS             complement(1886..2650)
                     /locus_tag="VGP27_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7855157.1"
                     /translation="MQIAGSSAIVVGGAGGLGEATVRRLHDAGAKVVVADLADDKGKE
                     LESELGVRYVRTDATSEESVNAAIAEAASLAPLRISVDTHGGPASGGRLIGKDGSPLD
                     LDGFKKTIEFYLTAVFNVMRLSAAAIAKSDPLEEGARGAIVNTASIAGYEGQIGQLPY
                     SAAKGGVLGMTLVAARDLSPLGIRVNTIAPGTINTPAYGKAADQLEQYWGQQVPFPKR
                     MGRSTEYAQLAQSIIENDYLNGEIIRLDGALRFPPK"
BASE COUNT          494 a          957 c          922 g          490 t
ORIGIN      
        1 aaaccgatca atgcatgacg aaagaggggg attgacatca tgactcaggt agcgcaggcg
       61 ctcgaggtga ggccgcagtc cggttggaca ggtgccgaga tccacggcat cgacctgacc
      121 aagccgctcg atgatcagca ggtcgccgag gttcgtgcgg cattgctgca gtggaaggtc
      181 gtgttcttcc gagaccagtt catcgaccac gacgaccacc tgcggttcgc ctcggcgttc
      241 ggcaaaccca cgcccgcaca cccgctgttc gactccatcc ccgacccgaa ttacccgacg
      301 atctatccga tctttcgcga ccgtttcaag gcgcggtacg gcagtagccg tgggttcgac
      361 aagccgcact ggcatgcgga tgtgaccgca gcggtcaatc cacccgccgc gtccatcctg
      421 agggcggagg tcatcccgcc gtacggcgga gacacacagt ggtcgaacac cgtcgccgcc
      481 tacgccggac tatccgcccc gttgcagcgc ctggccgaca aactccgtgc ggtgcaccgg
      541 ttttcaccgc cggacggcgc taatgcaacc gaggattacc agcggcgggt gaaccggcgt
      601 cctttggtga ccgagcatcc cgtcgtcagg gttcatcccg aaacggggga gaaggcgttg
      661 tatgtgaacc ccggtttcac gacacacatc gtcggtgtct cgccgcacga gagccgacga
      721 cttctcggcc tgttctacga cgagttggtc cggcccgagt acacggtgcg gttcaagtgg
      781 gagccgggca gcatcgcctt ctgggacaac cggtcgaccg ttcatctcgc gccgtcggat
      841 ctcacgatcg accacgaccg caggctttac cgggtgacat tggtgggtga tgttccgacg
      901 gggccggacg gccggcagtc ggtggctctc gacggggaac ctttcgaggc gatctgaccg
      961 caaccgcttc gctcgcacaa gcgggaggta ttcccgcttg cgagcgtgcg tgtctgctcc
     1021 gcgacacgcc ctcgcgcaga ggcaattcgc gcacgctcgc gatgccttga cggtgcgctg
     1081 actgccacga aaagcggcgt gttggcgtgc agacacgcac gctcgcggtg cggggcgggg
     1141 ggctagaagg tgagtgattc ggagcgctgg atggtgccgg tgatgccgcc cgcatcctgt
     1201 tgtgcgagaa gcacagccgc gttggccatc gcttccggcg gttcgaccag ctccggcgga
     1261 atctccatac cggcaccgcc ggcgtgccag ccctcggtca gcaccacgcg cgacgggctc
     1321 aggcaattga ccgcgatgtt atcgggcttc aggtccgcgg ccaagccgag atacaaccgc
     1381 tcgacgccgg ccttggacac ccaataggcg ttggcgccct ggtcggtcat cgtgacaccg
     1441 gtcgtcgtga cggccaccaa cgagccgccg ccgcgtgccc gcacattcgg tatgaccgcc
     1501 ttggtcgcca ggaagacacc ggtgaggttg acgtcgaggc acaactgcca gcgcttgagc
     1561 ggggtggcct cgatcggccc cagccacaac acccccgcgt tggcgaccag gatgtcgatc
     1621 ccgccgaact cggcgaccgt tgcggcaacg gctgcctgca ccgagtcctc tttggtgacg
     1681 tcgcacgtga cggccaatgc gcggccaccc gatgacgaaa tggtctcggc gacagtgtga
     1741 atggtgccgg gcaacttgcc ctcgtgttcg gagcgtgcgg caaccgccac cgccgcaccc
     1801 tcgcgcgcaa gcgccgcggc gaccgtcgcg ccgatgccgc gactggcgcc ggcgacgaag
     1861 gccaccttgc cctcgagcga agcgctcact tcggcgggaa gcgcagcgct ccgtcgaggc
     1921 ggatgatctc gccgttgagg tagtcgttct cgatgatgct ctgggccagc tgcgcgtact
     1981 cggtggaacg tcccatccgc ttgggaaacg gcacctgctg gccccagtac tgctcgagct
     2041 gatcggcggc cttgccgtag gcgggggtgt tgatggtgcc gggggcgatg gtgttgacgc
     2101 ggatgcccag cggcgacagg tcgcgtgcgg caaccagcgt catgcccagc acgccgccct
     2161 tggccgccga gtacggaagc tgaccgatct ggccctcata gccggcgatc gatgcggtgt
     2221 tgacgatcgc gccgcgggcg ccctcctcca gcgggtcgga cttcgcgatc gcggccgctg
     2281 atagccgcat cacgttgaac acggccgtca gatagaactc gatcgtcttc ttgaagccgt
     2341 cgaggtcgag cggtgagccg tccttgccga tgagccggcc gccacttgca gggccgccat
     2401 gggtgtcgac agagattcgc agcggtgcca gcgacgcagc ctcggcgatc gccgcgttga
     2461 cggactcctc ggaggttgcg tcggtgcgca catagcgcac acccaactcg ctctccagct
     2521 ccttgccctt gtcgtcggcg aggtcggcga caaccacttt ggcccccgcg tcgtgcagcc
     2581 tgcggacggt cgcctcacca aggccgcccg cgccgccgac cacaatcgcg gagctaccgg
     2641 cgatttgcat agaatttccc ttcttgcaac tgacactctc gatcagagag aataacattc
     2701 tcacgctgac cgaaacggag tcacccgatg ccagaagccg tcatcgtctc cgccaaagcg
     2761 tcgtagtcca tcatttgacg atgaccatcg acgagctcat caccgcggca cgcaacgggt
     2821 ccacccgagc ggccggacgg ttgttgagcc tggtcgaaag ctc
//
LOCUS       DASYLX010001181         9187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_650084,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001181 DASYLX010000000
VERSION     DASYLX010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650084"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGP27_27915"
     CDS             <1..595
                     /locus_tag="VGP27_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7855158.1"
                     /translation="VVGLAFVLLTVAFRAALVPITSIVGFLLSVFAALGVQVAIFQWG
                     WGADLLGVTPGETISFLPIIVLAIIFGLSSDYQVFVVSRMKEELTKTDDALDAVRSGV
                     GLSARVVTAAALIMFGVFVAFLAGGDPIIKSVGLTLAVGVFLDAFVVRLTLIPAIMSV
                     LGRRVWAHSKWFARYVPDVDIEGTKLESRDVQLSALR"
     gene            complement(678..1787)
                     /locus_tag="VGP27_27920"
     CDS             complement(678..1787)
                     /locus_tag="VGP27_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7855159.1"
                     /translation="MSIHPTNVVLASTRLCSSLPVIGKHLEPFAGLTAMALYGGHYAP
                     AAVRAAVMRRTGWGLASDDAEPVVEAAPVATAGSRIPPFVRASAQRRRCLHSSSVRYG
                     GHPAQLLDVWRRPDLAPGAPVLLFVPGGAWVQGTRVLQGHTLLSHLVEHGWVCLTMDY
                     RVSPVHRWPRHIADVNAAIAWARANIGQYGGDPDFVAVAGCSAGGHLASLAGLTPGDE
                     RFRGELAADADTSVDAVVSIYGRYDWEDRSTATRRNFQGFLERVVVGRRQSRHPEVYA
                     AASPMARIHEGAPPFFLIHGDQDTIIPVREARHFRQALEATSRNPVVYSEIPRAGHAF
                     DLVDTSHARRCAVEVTRFLDEVWTRHEESRPAAAV"
     gene            2201..2671
                     /locus_tag="VGP27_27925"
     CDS             2201..2671
                     /locus_tag="VGP27_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7855160.1"
                     /translation="MAHAPVDEQRCDRIGETLDQASDLAVRYLSDRSGLSLAASFVLN
                     RLHREGPSRLTALAAKEGVSQPSMTQLIQRMERHGLVTRLTDPEDGRVALVAITEAGT
                     TTLEDRRQVRRERLAELLTTLTPDEARALALAAEVALPIMRRLTWNADLDLQLD"
     gene            2683..3123
                     /locus_tag="VGP27_27930"
     CDS             2683..3123
                     /locus_tag="VGP27_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7855161.1"
                     /translation="MRGIPVGRWLKRLWIPVVLIVVLAVSGLVVSRLHRMFASEDLNA
                     NSGKGIEIVQFNPKVLVYDVYGAAGTTAQISYFDPAANVHLVTAPLPWSISLSTTLPT
                     VSANLMARSDSDEIGCRVTVNGVVREEKSANGVNAQTFCLVKSA"
     gene            3120..5996
                     /locus_tag="VGP27_27935"
     CDS             3120..5996
                     /locus_tag="VGP27_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7855162.1"
                     /translation="MTDTATRQHRRPVFAHSLRIFAIPIVLIWIAIAVVLNAIAPQLE
                     VVGELHAAPMAPEDAPSMIAMKRMGANFKEFNSNSTVMVVVEGQQPLGPDAHKYYDEI
                     IRKLRQDPEHIQHIQDFWGDTLTAAGAQSPDGKASYVMLNLAGEQGQTLANEGVQAVR
                     KVIEDTKAPPGVQAYVAGPAALTDDLHVIGNASLAQITLITLIAISVMLLVVYRSIIT
                     TLVQLFLTGIGLLTSRGMVSTLAMHDAFGLTTFAGNILTMLAIAAATDYGIFIFGRYR
                     EARGSGLDRDDAYYTTFRSVAPVIIGSGLTIAGATYCLSFARLPYFSTMGAPVAIGMI
                     VVVASAVTLGPAVLFLGSRVGLFQSKRPPTSRFWRRVGVAVVRWPAPIFVASLFVVLI
                     GIVAIPGYKPAYNDRYYLPDDAPVNIGFAAADRHFTQARMNPDILMIEADHDMRNPAD
                     MLVLNKVSGNVMHTEGIAMVQNITRPLGIPIQHSSIPFQTSVQGQTTNLNLPFQRDQL
                     ANQLKTVDAMNASINIMEKQYALSLLQSRLTQDSAAKSRDLLAKTEELRDDIANFDDQ
                     FRPLRNYFYWEPHCFDIPLCAAARSVFDALDGIDEVTDKTGAVQANTDKLADLAPQLT
                     ALMPQTIATMKTSRDLALASYNSQKALIDQMEASNDTALAMGQSFDTAKNDDLFYLPP
                     EAFDNPDFKRGLVMFLSPDGKSARMFITHESDPATPEGIARVDSERKAAQEALKMSSL
                     SNAKVYLGGVAATYKDMSDGAKYDLLIAVISSLTLIFMIMLILTRSAVAALTIVATAG
                     SSIAASFGISVLIWQNLLGIHVQWLVMLMSVIILLAVGSDYNLLLVSRFKDEIHAGLK
                     TGIIRSMAGTGGVVTAAGLVFAATMAAMCFSELTVLAQMGSTIAIGLLVDTLIVRSLL
                     MPSIATMLGRWFWWPQVVRPRGDNHLRRRPHSDDLDTEALPANA"
     gene            complement(6000..7364)
                     /locus_tag="VGP27_27940"
     CDS             complement(6000..7364)
                     /locus_tag="VGP27_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7855163.1"
                     /translation="MSKTTELTILDPRTGETVAHVPVADAQACERAVARARVAWDDWM
                     RTAAGERAAHIRDAAASVEAAAKELAELIERETGKPVDDALGGVQAGVDTLRQYAELG
                     PLHGGRSLQGDWAATDFMIAEPRGVVAVLTPWNDPVAVAAGLIGAALVVGNTIVHKPS
                     ERCPSAGRRFAELIAAHLPDGVLEIVDGDGTVGAMLAAADDVDVVAHVGSTATGRDIA
                     AACAARGAKAILENGGNDALIVDAGVDPPWAAGQAATGAFANAGQICVSVERIYVVEA
                     IAGQFIDALVREAGEWGGRIGPLVDQRHCEQVHRHVDDAISKGAAVMAGGQPDSGPGS
                     FYPPTVLTDCHPAMLVFSEETFGPVAAVRVVADFETALTEAAQDRYGLAATVLTPDMG
                     HAQQAWRTLPVGTVKINAVFGGAPGGASEPRKASGSGFGFGPELLDEMTAVKVVHWSA
                     PAAG"
     gene            complement(7410..7862)
                     /locus_tag="VGP27_27945"
     CDS             complement(7410..7862)
                     /locus_tag="VGP27_27945"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7855164.1"
                     /translation="MTIDNVLEQQKSVQNIDCRGASVSVESQGPTTVIAVSGDIDASN
                     SDFMTTVLSGFARRNDRVVVDLSSVDFIGTQGVRVLIDFDRQCRRDGAAWALVPCRIL
                     RRLLGVIDVGRHLPVADSVDDAVQLLQWGVISPDLQNLSRVAPEKLRC"
     gene            complement(8076..8474)
                     /locus_tag="VGP27_27950"
     CDS             complement(8076..8474)
                     /locus_tag="VGP27_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV7855165.1"
                     /translation="MLPALDEHNRAFWTGGGDGRLLVTWCAHCELWVQPPAGECPVCR
                     GALVARPVSGKGTVFTYTVNHQPFNPAVPVPYVIAIVELEEHTDLRIATNIVDCEADS
                     VYVGLPVEVRFERQAADGDNVFMPVFAPRS"
     gene            8570..>9187
                     /locus_tag="VGP27_27955"
     CDS             8570..>9187
                     /locus_tag="VGP27_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7855166.1"
                     /translation="MSYFEKDAIISGLGISRIGRRTGIPSRELTLESVRAAIEDAGLT
                     ASDIDGIATFGDTPPAEVLEALDIQAQDRSTGFDTGGVLIPVMSAFRAVSERRSRHVL
                     VYRTVAMLGGSMNQEASPLLNPLASPPVEPHAPGTRRKLKPFEDIDELVAAHSYSAAN
                     WLAMHCRRHMELYGTTKEQLGWLAVNSRRNAGLNPRAAYREPMTMD"
BASE COUNT         1624 a         3045 c         2899 g         1619 t
ORIGIN      
        1 ggtcgtcggg ctggcgttcg tgctgctgac cgtcgcgttc cgcgccgcgc tggtaccgat
       61 cacctccatc gtcggtttcc tgctatcggt gttcgccgcg ttgggggttc aggtcgccat
      121 cttccaatgg ggttggggcg ccgaccttct gggtgtcacc cctggtgaga cgatcagctt
      181 tctgcccatc atcgtgctgg cgatcatctt cggcctgtcc agtgactacc aggtcttcgt
      241 cgtctcgcga atgaaggagg agctcaccaa gaccgacgac gcgctcgacg cggtgcgcag
      301 cggtgtcggg ctgtccgcgc gagtcgtcac cgccgcggcg ctgatcatgt tcggtgtctt
      361 cgtcgcattc cttgccggcg gcgatcccat catcaaatcg gtcgggctca ccctggccgt
      421 cggcgtgttc ctcgacgcct tcgtcgtccg gctcaccttg atcccggcga tcatgagcgt
      481 cctgggccgc agggtgtggg cgcactcgaa atggttcgcg cgctatgttc ccgacgtcga
      541 catcgagggc acgaaactgg aatcccggga cgttcagctg tcggctttga ggtagcgggc
      601 acagagccgg ggcagcgcgt accgcatcag tctcgtcgac ccgctggcgg cggcgcgttg
      661 cacgacgacg acgcggatca gaccgcagcg gcgggacggc tctcttcatg acgagtccat
      721 acctcgtcga ggaagcgggt cacctcgacc gcgcatcgcc gcgcgtgcga ggtgtcgacg
      781 aggtcgaagg cgtgacccgc ccgcgggatc tcggagtaca caacgggatt gcgcgacgtc
      841 gcttcgagtg cctggcggaa gtgacgcgcc tcgcgcaccg gaatgatggt gtcctgatcg
      901 ccgtgaatga ggaaaaacgg cggcgcgcct tcgtgaatgc gcgccatcgg cgatgccgcg
      961 gcgtacacct caggatggcg agactgcctg cgccccacca cgacacgttc gaggaagccc
     1021 tggaagttcc tgcgtgttgc cgtcgaccgg tcctcccagt cgtagcgccc gtagatactg
     1081 acgacggcgt ccaccgaggt gtcggcgtcg gccgcgagtt caccgcgaaa cctttcgtca
     1141 ccgggtgtca ggcctgcgag cgacgccagg tggccgcccg ccgagcagcc ggcgaccgcg
     1201 acgaagtcgg gatccccgcc gtactgaccg atgttggcgc gcgcccatgc gatcgcggca
     1261 ttgacgtctg cgatatggcg tggccaccgg tgcaccggcg acacccgata gtccatggtc
     1321 agacacaccc acccgtgctc cacaagatgg gacaacaagg tgtggccctg caggacgcgg
     1381 gtgccctgca cccaggctcc gcctggcacg aacaacagca ccggcgcacc gggcgccagg
     1441 tcgggccgac gccagacgtc gagaagctgg gccgggtgac cgccgtagcg gaccgaactg
     1501 ctgtgcaggc agcgtcgccg ctgcgcgctt gcccgtacga acggaggaat tcggctgccc
     1561 gccgtcgcca ccggtgcggc ctcaaccaca ggctctgcgt catcggacgc gaggccccag
     1621 cccgtgcggc gcatgacagc ggcgcgcacg gctgcgggcg cgtagtgacc gccgtagagg
     1681 gccatcgccg tcagccctgc aaacggttcg aggtgcttac cgatcacggg aagcgacgaa
     1741 cagagcctgg tagaggccag tacgacattg gtcgggtgga tcgacatcgg ctttccttcg
     1801 caaaggggat cgtgtccgcc gactgtctgc agcgctgtct gcatttaccc gacgcttcgc
     1861 cgaccgaaac cacccaccgg ccgtcccgtt taaagggtgg ccagggtacg agtcgtcggt
     1921 gagcacgcga acacatcaac tgacgcctga cttcgccgcc gcgcctgtgc cgatgtcggc
     1981 acggtagcca atagcctgct tacatcatca caactatgta tgcagttcta cgccccgtgg
     2041 cagtgggata tcacggtgtc gccgacgacc tcgaatgtcg gctgtctaat tgctgtgagc
     2101 tgtagagacc ggtgactccc gcccctgccg ataatgaccg ccgaggcgaa tgaggttacc
     2161 cccgattgca agtacatacg tcacatgata ttaatccgat gtggctcacg ctccggtcga
     2221 cgaacagcgg tgtgaccgca tcggggagac gctcgatcaa gcttcagatc tcgcagtccg
     2281 gtatctcagc gaccggtcgg gcctgagcct ggcggccagt ttcgttctca acagactgca
     2341 ccgggaaggt ccttcacgcc tcaccgcgct cgcggcgaag gaaggcgtca gccagccttc
     2401 gatgacccaa ctgatccagc ggatggagcg ccacgggttg gtcacccggc tgaccgatcc
     2461 cgaggacggc cgcgtggcgc ttgtcgccat caccgaggca ggcacgacga ctctcgagga
     2521 ccgcaggcag gttcggcgcg agcgcctggc cgaactgctg acgacactca cccctgacga
     2581 agcgcgcgcg ctggcgctcg ccgccgaggt cgccttgccg atcatgcgcc ggctgacgtg
     2641 gaacgcagat ctcgatctgc agctggattg agaggcgacg aggtgcgagg cattccggtt
     2701 ggccgctggc tgaagcgcct gtggattcct gtagtgctca tcgtcgtgct ggccgtatca
     2761 ggtctggtcg tctcgcggct gcacaggatg ttcgcttccg aggacctcaa tgccaattcc
     2821 ggaaagggta tcgagatcgt gcagttcaac ccgaaggtct tggtgtacga cgtgtacggg
     2881 gccgccggga cgaccgccca gatcagctat ttcgacccgg cggcgaacgt acacctggtc
     2941 accgcgcctt tgccctggtc gatctcgctc tcgacgacct tgccgacggt cagcgcgaat
     3001 ctcatggcgc gcagcgacag tgacgagatc ggttgtcgcg taaccgtcaa cggagtcgtc
     3061 cgcgaggaga agtcagccaa tggggtcaac gcccagacgt tctgcctggt gaagtctgca
     3121 tgaccgatac cgctacccgg cagcacagac ggcccgtctt cgcccacagc ctccggatct
     3181 tcgcgatccc gatcgtcttg atctggattg ccatcgccgt cgtcctgaac gcgatcgccc
     3241 cgcaactcga ggtggtcggt gaactgcatg cggcgccgat ggcccccgaa gacgcgccct
     3301 cgatgatcgc catgaagagg atgggcgcca acttcaagga gttcaactcc aacagcacgg
     3361 tgatggtcgt cgtggaaggc cagcagccgc tgggccccga cgcacacaag tactacgacg
     3421 agatcatccg taagctgcgc caggaccccg aacacataca gcacatccag gacttctggg
     3481 gcgacacgct gactgccgcc ggagctcaga gcccggacgg caaagcctcg tacgtgatgc
     3541 tgaatctcgc gggcgaacaa ggccagaccc tggccaacga aggggtccag gccgtccgca
     3601 aggtgatcga agacaccaaa gcgccgccgg gagtgcaagc ctacgtcgcg gggcctgccg
     3661 cgctcaccga cgatctgcat gtcatcggca acgccagcct ggcgcagatc accttgatca
     3721 ccctgatcgc gatctccgtc atgttgctgg tcgtctatcg ctcgatcatc accacgctgg
     3781 tccagctgtt cctgaccggg atcggattgc tgacgtcgcg cggcatggtc tcgacgcttg
     3841 ccatgcacga cgcgttcggg ctgacgacgt tcgccggaaa catcctgacg atgctggcga
     3901 tcgccgccgc caccgactac ggcatcttca tcttcggccg ctaccgcgaa gcgcgtgggt
     3961 ccggcctgga tcgggatgac gcctactaca ccacttttcg gtccgtagcg cccgtcatca
     4021 tcgggtccgg cctgacgatc gcgggcgcca cttactgcct gagcttcgcg cggctgccct
     4081 acttttccac catgggcgca ccggttgcga tcggcatgat cgtcgttgtg gcgtcggccg
     4141 tcacgctggg acccgccgtt ctgttcctcg gtagccgggt cgggttgttc caatcgaaga
     4201 ggcctccgac aagcaggttc tggcgacgcg ttggtgtcgc cgtggtgcgg tggcccgcac
     4261 cgattttcgt ggcgagcttg ttcgtcgtac tgatcggcat cgtggcgatt cccggttaca
     4321 agccggccta caacgaccgc tactacctgc cggacgacgc cccggtcaac atcggtttcg
     4381 ccgccgcaga ccggcatttc acgcaggcca ggatgaatcc cgacatcctg atgatcgagg
     4441 ccgatcacga tatgcggaat ccggcggaca tgcttgtgct gaacaaggtt tcaggcaacg
     4501 tcatgcacac cgagggcatc gcgatggtcc agaacatcac gaggccgctc ggaatcccga
     4561 tccagcacag ctcgattccc ttccaaacga gtgttcaggg tcagaccacc aacttgaacc
     4621 tgccgttcca gcgggatcag ttggccaacc agctcaagac ggtcgatgcg atgaacgcct
     4681 cgatcaacat catggagaag caatacgcac tctcgctgtt gcagagccgg ctcacgcagg
     4741 actcggcggc caagtcgcgg gacctactgg ccaaaaccga agaactgcgc gatgacatcg
     4801 cgaacttcga tgaccagttc cggcccctgc gcaactactt ctactgggaa ccgcactgct
     4861 tcgacatccc cctgtgcgct gcggcgaggt ccgtcttcga tgccctcgac gggatcgacg
     4921 aagtgaccga caagacggga gcggtgcaag ccaataccga caagctggcg gacctcgcgc
     4981 cgcagctgac ggcactcatg ccacagacga tcgcgacgat gaagaccagc cgggacctgg
     5041 cgctggcgtc gtacaactcg cagaaggccc tgatcgacca gatggaggcc tccaacgaca
     5101 ccgcgctcgc gatgggtcag agtttcgaca cggcgaagaa cgatgacctc ttctatcttc
     5161 ctccggaagc gttcgacaac cccgacttca aacgcggcct ggtgatgttc ctatcgccgg
     5221 acggcaagtc cgcgcgcatg ttcatcactc acgaaagtga cccggcgacc cccgagggca
     5281 tcgctcgggt cgactcggag aggaaggccg cgcaggaagc actgaaaatg tcgtcgctgt
     5341 cgaacgcgaa ggtgtacctt ggcggcgtcg cggcgaccta caaggatatg tccgacggcg
     5401 ccaaatatga cctgctgatc gccgtgatct cgtcgctgac cctgatcttc atgatcatgc
     5461 tgatcctcac tcgcagtgcg gtggcggcgc tcaccatcgt cgcgaccgca ggcagttcga
     5521 tcgccgcatc attcggcatc tccgtgctga tctggcagaa cctgctgggc atccacgtgc
     5581 aatggctggt catgttgatg tcggtgatca tcctgttggc ggtgggatcg gactacaatc
     5641 tgctgctggt ctcccggttc aaagacgaga tccacgcggg tttgaagacc ggcatcatcc
     5701 ggtcgatggc cgggacgggt ggcgtggtga cggcagcggg gctggtgttc gccgcgacca
     5761 tggccgcgat gtgcttttcc gagctgactg tgctggctca gatgggctcc acgatcgcca
     5821 tcggcctgct ggtggacacg ctcatcgtgc gctcgctgct gatgccgtcg atcgcgacga
     5881 tgctgggccg gtggttctgg tggccacagg tggtacgccc ccgcggcgac aaccatctgc
     5941 ggcgacggcc gcacagcgat gacctagaca ccgaagcgct gcccgccaac gcgtaggtgt
     6001 cagccggcgg cgggcgcgct ccagtgcacc accttcacgg ccgtcatctc gtcgagcagt
     6061 tcgggcccga agccgaatcc gctgccgctg gccttccgcg gctccgaggc gccgcctggc
     6121 gccccgccga acaccgcgtt gatcttgacc gttcccaccg gaagcgtccg ccacgcctgc
     6181 tgtgcgtggc ccatatccgg cgtgagcacc gtagccgcaa gaccgtagcg gtcctgtgcg
     6241 gcttcggtaa gcgcggtctc gaagtccgcg acgacgcgca cggccgcaac aggtccgaaa
     6301 gtctcctcgc tgaacaccaa catggccgga tggcagtccg tcagcactgt cggcggatag
     6361 aaagagcccg gccccgaatc gggttggccg cctgccatta ccgcggctcc cttcgagatc
     6421 gcgtcgtcga cgtgccggtg tacctgctcg cagtgacgct gatcgaccag cgggccgatt
     6481 cggccgcccc attcaccggc ctcacgcacc agtgcgtcga tgaactgacc ggcgatcgcc
     6541 tccaccacgt agatgcgctc gacggagacg cagatctgtc cggcgttggc gaaggcgccg
     6601 gtggcggcct gcccggcggc ccacggggga tcgacgcccg cgtcgacgat gagggcgtcg
     6661 ttgccgccgt tctccagaat cgctttggcg ccacgggccg cacatgctgc ggcgatgtcg
     6721 cgaccggtag cggtgctgcc gacgtgggcg acgacatcga cgtcatcggc tgccgccagc
     6781 attgcaccga cggtcccgtc gccgtcgacg atctcgagga cgccgtcggg cagatgcgca
     6841 gcgatcaact cggcgaaccg cctgcccgcg gagggacacc gttcgctcgg tttgtgcacg
     6901 atggtgttac caacgaccaa ggcggcaccg atgaggcctg cggcgaccgc caccgggtca
     6961 ttccacggtg tcagcaccgc caccacgccc cgaggttcag cgatcatgaa atccgttgcg
     7021 gcccagtctc cttgtaggct gcggcccccg tgaagcgggc cgagttcggc gtactgccgc
     7081 aacgtgtcga cacccgcctg cacgccgccg agggcgtcgt cgaccggctt gccggtctcg
     7141 cgttcgatga gttcggcgag ctccttcgct gcggcctcga cgcttgcggc ggcatcccgg
     7201 atatgagccg cccgctcccc cgccgcggtg cgcatccagt cgtcccacgc cacccgggcg
     7261 cgtgcgacag cgcgctcaca tgcctgtgcg tcggcgaccg gcacatgggc gacggtctcg
     7321 ccggtacgcg gatccaggat cgtcagttcg gtggttttcg acactcctgt gtcttaccca
     7381 ggctgcagcg ggtgaatcat cgacgccgct cagcagcgca gcttctccgg agccacccgc
     7441 gaaaggttct gcaggtccgg cgaaatcaca ccccactgaa gtagctgtac cgcgtcgtcg
     7501 acggagtccg cgaccggcag atgccgtccg acgtcgatca ctcccaagag cctgcgaagt
     7561 atccggcagg gcacaagagc ccacgccgca ccgtctcgcc ggcattggcg atcgaaatcg
     7621 atcagcactc gaacaccctg cgtgccaatg aaatcgacac tgctcaagtc gacgacaact
     7681 cgatcgttcc tcctggcgaa accgctgagg acggtggtca tgaagtcgct gttcgatgcg
     7741 tcgatatcgc cgctcacggc gatgacggtc gtcggcccct gcgattcgac ggaaacagaa
     7801 gcgccgcggc aatcgatgtt ctggaccgac ttctgctgtt ccaagacgtt gtcgatggtc
     7861 atgttctcct cgcctgcttt gtggtgcagg ctgtgaactc aaccgcttgg ccaaggttac
     7921 cgtacttctg ccgtcaatga cgccgctgcg attctaaccg tccgccgagg gcggtcggag
     7981 ttttcgcttc ccgtaccgga atgccctgtg catcttcgcc aattggctcg ggcacatgcc
     8041 gtttggcgca tgaggcccgc ggtaccaagc gcgggtcatg agcgcggagc gaacaccggc
     8101 atgaagacgt tgtcgccgtc ggcggcttgg cgttcgaagc gcacctcgac cggaagcccg
     8161 acgtagaccg aatccgcttc acagtcgacg atgttggtcg cgatccgcag gtcggtgtgc
     8221 tcctccagct cgacgatcgc gatcacgtac ggcaccggca cggccgggtt gaacggttgg
     8281 tggttgaccg tgtacgtgaa gacggtcccc ttgcctgaaa ccggccgggc gacgagcgcg
     8341 ccgcggcaga ccggacactc cccggccggc ggttgaaccc acagctcaca gtgggcgcac
     8401 caggtgacca acagtcgacc gtcaccacct cccgtccaga acgcccggtt gtgctcgtcg
     8461 agcgcgggca acatccgggc gggaatctcg ggtggcgcct cgtctgccat aacgagcaaa
     8521 gtacagtcca accgactgtc cttacaaaaa tagagtttcg aggagcccga tgagctactt
     8581 cgagaaggac gcgatcatca gcggcctcgg catctcgcga atcgggcgcc gcaccggcat
     8641 ccccagccgc gagttgacgc tcgagtccgt acgcgcggcg atcgaggatg ccggtctgac
     8701 cgcgtcggac atcgacggta tcgccacatt cggggatacg ccgcctgccg aagtgcttga
     8761 ggctcttgat attcaggcgc aggaccggag cacgggcttc gacaccggcg gagtactcat
     8821 acccgtgatg tccgccttcc gggccgtatc ggaacggcgg tcgcggcatg tgctggtgta
     8881 tcggaccgtc gcgatgctgg gcgggtcgat gaaccaggag gcatcgccgc tgctcaaccc
     8941 gctggcctcc ccgcccgtcg aaccgcacgc tcccggaacc cggagaaagc tcaaaccgtt
     9001 cgaggacatc gacgaattgg ttgccgcgca ctcgtattcg gccgcgaact ggctggccat
     9061 gcactgcagg cggcacatgg agctctacgg aacgaccaag gagcagctgg gttggctggc
     9121 cgtcaacagc aggcgcaatg ccgggctcaa cccgcgcgcg gcctatcgcg agccgatgac
     9181 gatggac
//
LOCUS       DASYLX010001182         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2187535,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001182 DASYLX010000000
VERSION     DASYLX010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187535"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGP27_27960"
     CDS             complement(<1..186)
                     /locus_tag="VGP27_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7855167.1"
                     /translation="MTGAVRDFVPVRAGHVSIYLCGATVQGLPHIGHVRSGVAFDVLR
                     RWLMAKGFDVAFIRNVTD"
     gene            complement(252..746)
                     /gene="ispF"
                     /locus_tag="VGP27_27965"
     CDS             complement(252..746)
                     /gene="ispF"
                     /locus_tag="VGP27_27965"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV7855168.1"
                     /translation="MTPRVGVGTDVHPIEAGRPCWLLGLLFDGADGCAGHSDGDVAAH
                     ALCDSLLSAAGLGDIGEVFGTDDPRWAGVSGAEMLRHVHGLVRGEGYRVGNAAVQVIG
                     NRPKVGPRRAEAQRLLSGLLDAPVSVSATTTDGLGLTGRGEGLAAIATALVVSDDENS
                     RIGR"
     gene            complement(743..1405)
                     /gene="ispD"
                     /locus_tag="VGP27_27970"
     CDS             complement(743..1405)
                     /gene="ispD"
                     /locus_tag="VGP27_27970"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV7855169.1"
                     /translation="MSTVAVVPAAGSGERLAAGAPKAFVNLGGRPMLEYVLRGLRDSG
                     VVDHVVVAVPPNRTDEARLVFGGDAVIVAGGSDRAESVRLALAAVGDAEFVLVHDAAR
                     PLTPPSVIVRVVHALQSGHLAVIPALPVSDTIKAVDANGVVLGTPERSGLRAVQTPQG
                     FRTELLRRAYERAAGLFTDDASVVEATGTPVQVVDGDPLAFKITTPTDLLLAQTLLGR
                     PA"
     gene            complement(1409..1897)
                     /gene="carD"
                     /locus_tag="VGP27_27975"
     CDS             complement(1409..1897)
                     /gene="carD"
                     /locus_tag="VGP27_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     CarD"
                     /protein_id="HEV7855170.1"
                     /translation="MIFKVGDTVVYPHHGAALIEAIETRTIKGEQKEYLVLKVAQGDL
                     TVRVPAENAEYVGVRDVVGQEGLDKVFQVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRDQERGLSAGEKRMLAKARQILVGELALAENTDDAKAEIILDEVLAA
                     AS"
     gene            2217..2810
                     /locus_tag="VGP27_27980"
     CDS             2217..2810
                     /locus_tag="VGP27_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855171.1"
                     /translation="MNRFKSRRSAVTVALAACGLAVAALSGCSAGQVAQTASQQAAVN
                     GAAATVGSISLRNVHIRAPQSGDYVQPGSEVELLFVASNESPDRPDKLTSITSDAGTV
                     TLSGDTSLPPGGVLVVGEPDGQIAALEQAETAAAAKATVTINKPITNGLTYNFTFTFE
                     KAGQITVAVPISAGEAGRRDQAGNTPNEEGGDTGGHH"
BASE COUNT          504 a         1031 c          942 g          447 t
ORIGIN      
        1 gtcggtgacg ttgcgaatga acgcgacgtc gaaacccttc gccatcagcc agcggcgcag
       61 cacgtcgaag gccactccgc tgcgaacgtg accgatgtgc ggcagaccct gcacggtggc
      121 accgcacagg tagatcgaca cgtggccggc acgcaccggc acgaagtcac ggacggcgcc
      181 cgtcatcgtg tcgtatagcc gcaacggctc atgcgctcct cgcgcgcgtg gtccggtcac
      241 gacgggccag cttaccggcc tattcggctg ttctcgtcat cgctgaccac cagcgcggtc
      301 gcgatggcgg ccaatccttc gccgcgaccc gtcaacccca ggccgtcggt ggtcgtcgcc
      361 gacaccgata ccggggcgtc caacagcccg gaaagcagcc gttgcgcctc tgcacgcctc
      421 ggcccgacct tgggtcgatt gccgatcacc tgcacagccg cgtttcccac ccggtagccc
      481 tcgccgcgta ccaggccgtg cacatgccgc agcatctctg caccgctgac accggcccag
      541 cgagggtcgt cggtgccgaa cacctcgccg atatcgccga gcccggcagc gctcagcagc
      601 gagtcgcaca gcgcatgcgc cgcgacgtcg ccgtcggagt gtccggcgca gccgtcggcg
      661 ccgtcgaaaa gcaggcccag cagccagcag ggccttcctg cctcgatcgg gtgtacgtcg
      721 gtgccgacgc cgactctcgg cgtcatgcgg gccgtcccag cagcgtttgc gcgagcagaa
      781 gatcggtcgg cgtggtgatc ttgaacgcca aggggtcgcc gtcgaccacc tgaaccggtg
      841 tgccggtcgc ctcgaccacc gaggcgtcgt cggtgaagag gcccgcggcc cgctcgtagg
      901 cccggcgaag cagctcggtc cgaaaaccct ggggggtctg cacggcacgc agcccagacc
      961 gttcaggggt acccagcacg acgccgttcg cgtcgaccgc cttgatggtg tcgctgaccg
     1021 gcagcgcagg gatcaccgcg agatgaccgg actgaagggc gtgtaccacc cgaacgatca
     1081 ccgacggcgg tgtcagcggc ctcgccgcgt cgtgcacgag gacgaactcc gcgtcaccca
     1141 ccgcggcgag agcgagccga acggactccg cgcgatccga cccgcccgcg acgatgacgg
     1201 cgtcgccgcc gaacaccagc ctggcctcgt cggtgcgatt gggcggaacg gcgacgacca
     1261 catggtccac gacgcccgag tcacgcaggc cgcgcaggac gtactccaac atcgggcgac
     1321 cgcccaggtt gacgaaggcc ttcggcgcac ccgcggccaa ccgctccccg gatcctgcgg
     1381 ccgggacgac ggcgaccgtg ctcatccgtc aggacgcggc ggccagaacc tcgtcgagaa
     1441 tgatctcggc cttggcgtcg tcggtgttct cggcgagcgc cagttcgccg accaggatct
     1501 gccgggcctt ggccagcatc cgcttctcgc ctgcggacag gccgcgctcc tgatcacggc
     1561 gccacaggtc gcgaacgacc tcggcaacct tgttcacatc gccggaagcc agcttctcga
     1621 ggtttgcctt gtagcggcgc gaccagtttg tcggctcctc ggtatggggg gcgcgaagca
     1681 cctggaacac cttgtcgagg ccctcctgcc cgaccacgtc gcggacgccg acatattcgg
     1741 cattctcggc ggggactcgg actgtgaggt ctccctgggc aaccttcaag acgagatatt
     1801 ctttctgttc gcctttgatg gtccgggttt cgatcgcctc gatcaacgca gcgccgtggt
     1861 gtggatacac aacggtgtct ccgaccttga aaatcatctg tttcgagccc ctttcgatac
     1921 tccatactaa cacggggccc ggacacgcgt cgcacaacgg tgcaggtcag gggccccgca
     1981 ggtgaagagc gggggttgac acggtgacga atacgtgcaa ggcaccggct ggtcagcgac
     2041 cctaacgcga cccctcccac gtgcctgtgg atggttcgcg gcaaacccgg ataggggacc
     2101 tcggcacctc cgctaccgcc gccaaccacc acctactact gtgcatagtc gaacaggtcc
     2161 tcgtcactgg cgacccatgt caccggcgcg tcgccggtcg agcaggaggc ttctgggtga
     2221 accgcttcaa gtcccgccgt tccgcagtca ccgtcgccct ggcggcgtgt gggctggctg
     2281 tcgccgctct ttccggctgc agtgccgggc aggtcgccca gaccgcgagc cagcaggcgg
     2341 cggtcaacgg cgccgcagca acggtcggca gtatttcgct ccgaaacgtg cacatacggg
     2401 ccccgcagag cggtgactac gtgcagccgg gcagcgaagt ggagttgctg ttcgtggcgt
     2461 ccaacgaatc gccggaccgc ccggacaagc tgacatcgat cacgtccgac gcaggcacgg
     2521 tgacgctgtc cggcgacact tcgctgccac cggggggcgt actggtcgtc ggtgaacccg
     2581 acggtcagat cgcagccctg gagcaggcgg agaccgccgc tgccgcgaag gccacggtca
     2641 ccatcaacaa gccgatcacg aacggcctga cctataactt caccttcacc ttcgaaaagg
     2701 cgggccagat caccgtcgcg gtgccgatct cggcgggcga ggcggggcgc cgcgaccagg
     2761 ccggcaacac gcccaacgaa gagggcggcg acacgggcgg tcaccactaa ggatctgcct
     2821 tgatctgtca cagctggcgg ataccgtcac atcgtggcca aggcgcgttc gcagtaccgg
     2881 tgttccgagt gccaccacat caccgcaaag tgggtcgggc gctg
//
LOCUS       DASYLX010001183         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1090704,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001183 DASYLX010000000
VERSION     DASYLX010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090704"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VGP27_27985"
     CDS             complement(<1..522)
                     /locus_tag="VGP27_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEV7855172.1"
                     /translation="MGTELTAERLQRRLGGEAGADTGSDGDADDSDAALSRWLPDAPT
                     GDSAGWLAAVRADPGRAGAIALGLVGVVAVLVTVFTLLRDKTPAVVSAKLPPVEMVST
                     AQPTPAAPAAQGPVIVSVVGLVNKPGLVTLSTGARIADAVSAAGGALDGADLIGLNMA
                     RRVADGEQIIVGIA"
     gene            complement(724..969)
                     /locus_tag="VGP27_27990"
     CDS             complement(724..969)
                     /locus_tag="VGP27_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855173.1"
                     /translation="MNTTIARSFTRYIALPVVSAGILCGAALGLAGMANAATTTTEHS
                     TGSAIVASPDTHAKPAPNALPGWRHHHGIGRFDMFNQ"
     gene            1155..1781
                     /gene="nthB"
                     /locus_tag="VGP27_27995"
     CDS             1155..1781
                     /gene="nthB"
                     /locus_tag="VGP27_27995"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEV7855174.1"
                     /translation="MDGVHDLGGLDGFGPVEHTGSEPMFAADWERRTFRMMIGWIASL
                     GGPGGKFRHSIERMVPAHYLSSPYYEHWLTGLTTLAVEAGLTTPEDLDRRAGGRFPLS
                     QSDRGVLPDDLTSRTTPRFAIGDTVRVREQHPPGHTRAPRYVQGRRGTVVRVDEAAAI
                     DDIEAHGGGSVTDPLYSVRFASRALWGEAGTDGDSVHVDLFERYLQEA"
     gene            1781..>2290
                     /gene="nthA"
                     /locus_tag="VGP27_28000"
     CDS             1781..>2290
                     /gene="nthA"
                     /locus_tag="VGP27_28000"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEV7855175.1"
                     /translation="MADHHHPEALAPVEARIAAIEAVLVEKGILDVDAVDQIVAHFED
                     NLGPMNGARVVARAWVDPEYRTRLLADGTAAVAELGFGGPEGEYVVVLENTPTVHNLV
                     VCTLCSCYPWPILGLPPKWYKAPAYRSRAVREPRKLLAEMGTVIPDDVEVRVWDSSAE
                     ARYLVLPMRP"
BASE COUNT          334 a          728 c          839 g          389 t
ORIGIN      
        1 ggcgatgccg acgatgatct gctcgccgtc ggcgacgcgg cgagccatgt tcagcccgat
       61 caggtcggcg ccgtcgaggg cgccgcctgc cgcgctgacc gcgtcggcga tgcgcgcacc
      121 cgtggacaag gtcaccaggc ccggcttgtt cacgaggccg acaacgctga cgatgaccgg
      181 gccctgggcg gcgggcgcgg ccggagtggg ctgcgccgtc gaaaccatct ccaccggcgg
      241 tagtttggcc gagaccaccg ccggggtctt gtcccgaagc agtgtgaaca cagtgaccag
      301 cacggccacc acaccgacga gtcccagcgc gatggcaccc gcgcgaccgg ggtcggcgcg
      361 cacggccgcc agccaccccg cggagtcgcc ggtgggggcg tcgggcagcc agcgcgacag
      421 cgcggcatcg gagtcgtcgg cgtcgccatc cgagccggtg tcggcgccgg cctcgccacc
      481 gagcctgcgc tgaagccgct cggcggtcag ttcggtcccc atgtgcccga cggtaggtgc
      541 gaccgtggcg acaacggccg gtcggcagcg cattcggcgc gaactgtgaa tgaagtcggt
      601 tctgtggaca acttccccgt tgcttcgttc ctcgcgtgcg cggccacacg aggggggccc
      661 tccccggatg ttgaccgcag ccggggaggg cctctatgtg ggtggatcgt gttcggtggt
      721 ggcttactgg ttgaacatgt cgaatcggcc gatgccgtgg tggtgacgcc acccgggcag
      781 cgcgttgggt gcgggcttgg cgtgagtgtc gggcgaggcc acgatggccg atccggtgct
      841 gtgctcggtg gtcgtcgtgg cggcgttggc catgccggcc aggccgaggg cggcaccgca
      901 gaggatgccg gctgagacga ccgggagagc gatgtagcgg gtgaagctgc gggcgatggt
      961 cgtgttcatt ttgtatctcc ttgtgctggt tggcttttca gttcttctgc cggggtcttc
     1021 cggtgatgag ataactgtgc ctcagcggcg tgcagatgtg tgtcggccga atggccgtca
     1081 gaccggttga accgggtgtc ccccgatcgg gggaatacgg tcggatgccg ccgtcaagtt
     1141 cgtatgatcc ggacgtggac ggtgtgcacg acctcggtgg gctggacggc ttcggtccgg
     1201 tcgagcacac cggcagcgag ccgatgttcg cggcggactg ggagcggcgt actttccgca
     1261 tgatgatcgg gtggatcgca tctctcggcg gccccggtgg caagttccgg cactcgatcg
     1321 agcggatggt tcccgcgcac tacctgtcgt ccccgtacta cgagcactgg ctgaccggcc
     1381 tcaccacgct tgcggtcgaa gcagggctga cgacgccaga agacctggat cgtcgcgcag
     1441 gcggccgttt tccgctgtca caaagcgacc gcggcgtcct gcccgacgac ctcacgtccc
     1501 ggaccacgcc caggttcgcg atcggcgaca cggtgcgggt gcgcgagcag catccccctg
     1561 gtcacactcg cgcgccccgc tacgtccagg gcaggcgcgg caccgtcgtg cgggtcgatg
     1621 aggcagcggc catcgacgac atcgaggcgc acggcggcgg ttcggtcacc gatccgctgt
     1681 attcggtgcg cttcgcgtct cgcgcgttgt ggggcgaggc gggcacggac ggcgactcgg
     1741 tgcatgttga cctgttcgag cgatatctgc aggaggcatg atggcggacc atcaccatcc
     1801 cgaagcgctc gcacctgtcg aggcgcgcat cgcagccatc gaagcggtgc tggtcgaaaa
     1861 agggatcctc gacgtggatg cggtggacca gatcgtcgcg catttcgagg acaacctggg
     1921 accgatgaac ggtgccaggg tcgtcgcccg ggcgtgggtt gaccccgagt acaggacgcg
     1981 gctgctcgca gacggcaccg cggctgtggc ggaactcggc ttcggcgggc cggagggcga
     2041 atacgtggtg gtgctggaga acacgccgac agtgcacaac ctcgtcgtct gcacgctgtg
     2101 ctcgtgttac ccctggccga tactcgggtt gccgccgaag tggtacaaag cgccggccta
     2161 ccgttcgcgc gcggtgcgtg agccccgcaa gctgctcgcc gagatgggca ccgtcatccc
     2221 cgacgacgtc gaggtccggg tctgggattc cagtgctgag gctcgttacc tggtgctgcc
     2281 gatgcgcccg
//
LOCUS       DASYLX010001184         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_324278,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001184 DASYLX010000000
VERSION     DASYLX010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324278"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VGP27_28005"
     CDS             complement(<1..161)
                     /locus_tag="VGP27_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEV7855176.1"
                     /translation="MSNAVCKDYTEITDVRASDPQAVERAWQARTIRPAVRGNGRLMI
                     VAADHPARGA"
     gene            complement(154..1125)
                     /gene="iolC"
                     /locus_tag="VGP27_28010"
     CDS             complement(154..1125)
                     /gene="iolC"
                     /locus_tag="VGP27_28010"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEV7855177.1"
                     /translation="MTSTQPFDVLAIGRSGVDIYPLQIGVGLEHVESFGKFLGGSAAN
                     VAVAAARLGNRAALISGVGDDPFGTFVRNELADLGVDNRYMATHDEYPTPVTFCEIFP
                     PDNFPLYFYRRPSAPDLQITPDELDLDAVRSARLYWSTVTGLSEEPSRSAHFAAWEAR
                     QRASLTVLDLDYRPMFWESPAAATKQVQQALRHVTVAVGNREECEIAVGESNPHRAAD
                     ALLDLGVELAIVKQGPRGVLGKTKRSHVSVPPNDVDVVNGLGAGDAFGGSLCHGLLHG
                     WPLEKTLRYANAAGAIVASRLECSTAMPTAAEVAELADQTAVEAVNV"
     gene            1278..>1763
                     /locus_tag="VGP27_28015"
     CDS             1278..>1763
                     /locus_tag="VGP27_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7855178.1"
                     /translation="MPLAVELDRSSPVPLYYQLAQAIEAAIRDGELAPGDRFENELAL
                     AKRLTLSRPTTRRAIQELVDKGLLVRKRGVGTQVVQSSVHRRVELTSLFDDLTRAGQE
                     PTTKLLDYRVGPPDEEVARELSLKDDREVVAIHRLRSANGEPLAVMTNHLPVGIAPDA
                     EE"
BASE COUNT          294 a          559 c          614 g          296 t
ORIGIN      
        1 gcgccgcgtg caggatggtc ggcggcgacg atcatcagcc tgccattgcc gcggacggcg
       61 ggtcggatgg tgcgcgcctg ccacgcgcgc tcgacggcct gcggatccga cgcgcgcacg
      121 tcggtgatct cggtgtagtc cttgcatacg gcgttagaca ttgacggcct ccaccgcggt
      181 ctggtcggcg agctcggcga cctcggcggc cgtcggcatc gccgtcgagc attcgagccg
      241 cgatgcgacg atcgcgcccg cggcattggc ataccgcaac gtcttctcca ggggccagcc
      301 gtgcagcagg ccgtgacaca ggctgccgcc gaaggcatct ccggcgccga gcccattgac
      361 gacgtcgacg tcgttgggcg gcacgctgac gtgtgagcgc ttcgtcttgc ccagcacgcc
      421 gcgcgggccc tgcttgacga tggccagctc gacacccagg tcgagcagag cgtcggcggc
      481 ccggtgcgga ttgctctcgc caaccgcgat ctcacactcc tcacggttac cgacggcgac
      541 cgtcacatgc cgtagcgcct gctgcacctg cttggtcgcg gcggcgggcg actcccagaa
      601 catcggccgg tagtcgagat cgaggactgt caacgaggcg cgttgacggg cctcccacgc
      661 ggcgaagtgc gcgctgcggc tcggctcttc ggaaaggccg gtgaccgtcg accagtagag
      721 cctggcagag cgcacggcgt cgaggtcaag ctcgtcaggg gtgatttgga ggtcgggcgc
      781 acttggacga cggtagaagt agagcgggaa gttgtccggc gggaagatct cgcagaacgt
      841 caccggcgtc gggtactcgt cgtgggttgc catataccgg ttgtcgacgc cgaggtcggc
      901 cagctcgttg cggacgaacg tgccgaacgg gtcgtcgccg acgccggaga tcagcgcggc
      961 gcggttgccg agccgggcgg ccgccacggc gacgttggcg gcgctgcccc cgaggaactt
     1021 gccgaacgat tcgacgtgct cgagtccgac accgatctga agggggtaga tgtcgacgcc
     1081 gctgcgcccg atggcgagaa cgtcgaacgg ttgagtgcta gtcaagatcg ctctccggcg
     1141 gttggtggtg gcttgcgtat gactgtgcac tgcgacactc aagcatgtca atagtttgtc
     1201 ctgacattct gacttttgat catacggtgt tagtattcag ctaccgtgat ctggtcggac
     1261 ccgagatcgg agacaacgtg cccctggcgg tggagctcga caggtcaagt cctgtccctc
     1321 tctactatca gctcgcccaa gccatcgagg ccgcgatccg cgacggtgaa ctcgcaccgg
     1381 gcgatcggtt cgaaaacgaa ctggcgctgg cgaaaaggct gacgctgtcg cggccgacaa
     1441 cgcgccgggc catccaggaa ctcgtcgaca aagggctgct ggtccgcaag cgcggcgtcg
     1501 gcactcaggt cgtgcagagc tcggtgcatc gccgcgtcga gttaacgagc ctgttcgacg
     1561 acctgacgcg ggccggccag gaaccgacca cgaagctgct cgactatcgc gtcggcccgc
     1621 ccgacgagga ggtcgcgcgc gagttgagct tgaaggacga ccgcgaagtc gtggcgattc
     1681 accgcttgcg ctcggccaac ggcgagccgt tggcggtgat gacgaaccat ttgccggtcg
     1741 gcatcgcccc ggatgccgag gag
//
LOCUS       DASYLX010001185         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3176505,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001185 DASYLX010000000
VERSION     DASYLX010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176505"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VGP27_28020"
     CDS             complement(<1..735)
                     /locus_tag="VGP27_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7855179.1"
                     /translation="MAGPVGLPVIDTMIGFPHEGSAQYDFIRKQTKDRESKETFEFPV
                     EYMFKDVPQGLPTDEPVSLVLQQMDRFGIEKAMIGVSEGSAELALKTFPDRFVPSGAI
                     ADPNDVMGSVNAIRREYEQFGIAATSVFPAGTFPQVPIDDPKMYPIYATCVELGVPIF
                     VCAGVPGPRVPFTPQEVSRIDIVMFDFPDLVFVTRHGCEPWEDLAVKLMLKWPNLYYS
                     TSAFAPKYYPKAIIDYANSRGADKVLY"
     gene            959..1552
                     /locus_tag="VGP27_28025"
     CDS             959..1552
                     /locus_tag="VGP27_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7855180.1"
                     /translation="MSLADMTSGQLARRARIIEAVIDLIGAVGADAVQMRDVASRSGV
                     ALATVYRYFSSKEHLLAAALQDWQKRLTRRILAAGGPADEGPLPGVLDYLRRAQRAFY
                     GNPEMTALMLQAMTSTAPEAKAAIDHMGRTNVEMFDRLLEGLAPEEIADVSFGLNAAL
                     TSALTAVLTGALTLEESLSRVEWVARTLIGATTHRTV"
     gene            complement(1537..4445)
                     /locus_tag="VGP27_28030"
                     /pseudo
     CDS             complement(1537..4445)
                     /locus_tag="VGP27_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354059.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
     gene            complement(4442..>4597)
                     /locus_tag="VGP27_28035"
     CDS             complement(4442..>4597)
                     /locus_tag="VGP27_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEV7855181.1"
                     /translation="LQTTAPSAAPNIVAQGDGGSITCRITVDDEVKDERTSDGLNAQT
                     FCYVKSA"
BASE COUNT          858 a         1371 c         1503 g          865 t
ORIGIN      
        1 gtagagcact ttgtccgcgc cgcgtgagtt cgcgtagtcg atgatggcct tcgggtagta
       61 cttgggcgcg aacgccgatg tcgagtagta caggttcggc cacttcaaca tcagcttgac
      121 ggcgaggtcc tcccacggct cacagccatg gcgggtgacg aacaccaggt cggggaagtc
      181 gaacatgacg atgtcgattc gtgaaacctc ttgcggcgta aacggaacgc gggggccggg
      241 aacgccggcg cagacgaaga tggggacgcc cagttcgacg caggtggcat agatcgggta
      301 catcttcgga tcgtcgatcg gcacctgagg gaacgtgccg gccgggaaca ccgacgtcgc
      361 ggcgatgccg aattgctcat actcgcggcg gatggcgttg accgaaccca tcacgtcgtt
      421 cgggtcggcg atggcgccgg aggggacgaa gcggtcgggg aacgtcttca gcgccagctc
      481 ggcagagccc tcgctgacgc cgatcatggc cttctcgatg ccgaaccgat ccatctgctg
      541 cagcaccagc gacaccggct catccgtcgg caagccctgg ggcacatcct tgaacatgta
      601 ctcgacgggg aattcgaagg tctctttgga ctcccggtct ttggtctgct tgcggatgaa
      661 gtcgtactgc gccgagccct cgtgagggaa gccgatcatg gtgtcgataa ccggcagtcc
      721 gacaggtccg gccatggcat cctccgatgg gtgcgagcgc gacagcaccg tcgctgtccg
      781 atcaatattg aaacaaggtt ctacctatgt caagattgcg tgtagcgttc tagctgctgt
      841 aacagcggtc agagtcaact gttgtgctat atatcgaacg gactcgagaa acgctgttct
      901 cgatgttgga ggtcggcccg tcaacaccaa aagccctgac cacactcagg ccgacatctt
      961 gtcgctggcg gatatgacca gcgggcagtt ggcgcggcga gccaggatca tcgaggccgt
     1021 gatcgacctg atcggcgccg tgggtgccga tgcggtacag atgcgcgatg tggcttcgcg
     1081 ttcaggggtc gcactggcca ccgtctaccg atacttcagc tcgaaagagc atctgttggc
     1141 ggcggcgctg caggattggc agaagcgatt gaccaggcga attctggcgg ccggtggtcc
     1201 ggctgacgaa ggaccgttgc ctggcgtact ggactatctg cgacgcgctc agcgggcctt
     1261 ttatggcaac cccgagatga cagcgctgat gcttcaggcg atgacgtcga ccgccccgga
     1321 ggcgaaagca gcgatcgacc acatggggcg tacgaacgtc gagatgttcg accgcctgct
     1381 ggaaggcctt gcgccggagg agatcgccga tgtgagcttc ggcctcaatg ccgcgttgac
     1441 cagtgccctg actgccgtgc tcaccggagc gctgactctg gaggagtcgc tgagccgggt
     1501 cgagtgggtg gcgcgcacct tgatcggtgc gacgactcac cgaacggtct gaccgatccg
     1561 gctttccagc tgcgcggtcg gctgcgtcgg ccatggcgag ggcaccggcc ggtcgcgcac
     1621 ccgcatcggc caccagaacc acttgcccat cagcgccgct atggacggcg tcatgaacga
     1681 ccggatcacc agggtgtcga agagcaggcc gaggccgatc gtggtgccca cctgcccgat
     1741 caccgtcaac tcgctgaccg ccatcgacat catggtgaac gcgaagacca gcccggcaga
     1801 cgtcaccacc gaaccggtgc cacccatggc ccggatgatt ccggtgcgta gtccggcgtg
     1861 gagctcctcc ttgaagcgtg agaccagaag caggttgtag tccgcaccca ccgccaacag
     1921 gatgatgacc gacatggcca gcaccatcca gtgcagctcg ataccgagaa ggtgctgcca
     1981 gatcagcacc gataggccga acgaagcgcc cagcgacagc accacggtgc cgacgatcac
     2041 cgccgacgcc acgatgctgc gtgtgatgat caacatgatg atgaagatca aggccagcgc
     2101 ggcgaccgcg gcgatgatga ggtcgtatgt gttgccgtcg gacaggtcct tgaacgtcgc
     2161 ggcgctcccg ccgaggtaga tcgtcgaccc ctccagcggc gtgcccttga tggcttcctt
     2221 ggccgccttc ttgatcgcat cgattctcgc gatgccctcg gcggaaagcg ggtcgccgtc
     2281 gtggctgatg atgaaccgca cggcgtgacc gtcgggcgaa aggaactgct ccataccgcg
     2341 tttgaagtcg gcgttgccga acacctccgg cggcaggtag aacgagtcgt cgttcatcga
     2401 attgtcgaac gcctcgccca tggccgacga gttgccctgc atagcagcca tctgatcctg
     2461 catgcccttc tgtgaggcat acatcgtcag catcatggtc ttcatcgtct tcatggtgtc
     2521 gatcatctcg ggcatcatcg agaccatctg cggcataaga gaatccaggc gttccatctc
     2581 cggaatcagc tgctggatgt cgtcggtcat ggtgtcgacg ccgtcgaggg cgtcgaagat
     2641 cgcccgaaac gaatgacaga ccgggatgtc gtagcagtgc ggttcccagt agaaataggc
     2701 ccggatcggt cggaagaaat cgtcgaaatc ggcgatgttg tcccgcaatt cggccacgtc
     2761 gagggtcatg tccttcatct tggccaccat gttgtgcgtg gtgtctgcca tctccgcggt
     2821 gaggttggac atccgagtca tcgtgtcgat cgtgtgctgc atctcgtcag cctgcaccag
     2881 catgtcggcc atccggtcct gcagatactt ctggttcaac tgctgggtgg tgccttgcat
     2941 gctgatctga aatgggatcg acgtgtgttc gatcggcttg ccatcgggac gggtgatggc
     3001 ctgcacacgg gatatgccct cgacgcggaa gatcgccttg gcgatcttgt caatcaccag
     3061 gaagtccgac gaattgcgca ggtcgtggtc gctttcgacc atcagcagtt ccgggttcat
     3121 ccgcgcttgg gagaagtgcc ggtcggcggc ggcgtagccc tcgttggcgg gcaggtcggc
     3181 aggcagatag gagcggtcgt tgtagttggt cttgtagccg ggcagggtca acagcccgat
     3241 cagcgaaagc gcgatggtgg ccaccagaat tgcgccgggc cagcgcacga cgacggcgcc
     3301 gaccttgcgc caaccttggg tgcgcagcgc gcgcttgggt tcgagcgtct tgccgaaccg
     3361 gctcgcgacc gagatcaggg cgggaccgag tgtcaacgcg gcgaacacgg tgatgaccat
     3421 tccgatcgcc aggggtatgc ccaacgtctg gaagtacggc agccgggtga agtgcaggca
     3481 gaaggttgcg cccgcgatgg tcaggccgga accgagcacc acgtgcgcgg tgccgccaaa
     3541 catcgtgtaa tacgcctgtt ctcggtcctc gcccagcgag cgagcctcct gatagcggcc
     3601 gatcaggaag atggcgtagt cggtggcggc ggcgatcgcc agcgtcacaa ggaggttggt
     3661 cgcgaatgtc gaaagaccga tgatctcgtg aaaaccgagg aatgcaacca ggccgcgggc
     3721 ggcggccagt tcgaggacca ccatcaccag gacgatcaac accgtgatga tggaccggta
     3781 gaccagcagc agcatgatga tgatgacggt gaaggtcccg cctcgatcag ctgcatgctg
     3841 cggtcgcctg cgatgtgctg atcggcggcc aatgctgcgg gaccggtcac atacaccgtg
     3901 acgccctgcg gtggttgcat gctgtcgatg aggtgctgca cggactcgac ggactcgttg
     3961 gcaagcgcct cgccctggtt accggccagc ttcgcctgaa cgtaggccgc cttgccgtca
     4021 ttgctctggg cgccggtggc ggtgagcgga tcgccccaga agtcctgcac cgactgcaca
     4081 tgtcgggtgt cggacctgag ccgtttgatc atctcgtcgt agaaggcgtg tgcctcgttg
     4141 ccgagcgtgt tctggccctc gatcacgatc atcgctgagc tgtccgagtc gccttcggcg
     4201 aagagctcgc cgaccttctt catcgagatc atcgacggtg cgtcgttcgg gctcatggag
     4261 accgcgcgca tctggccgac ggtttccagc tggggaacga tgacgttgaa gatcgcgatg
     4321 acggcgatcc agccgatgac gatcggcacc gccatactcc ggatcacccg cggaatgaac
     4381 gggcgcttcg cgtgcctggc gggcgggaag gcgtcggtcg gcgcgtcggt gctgggagcg
     4441 ctcacgcgga cttcacatag cagaaggtct gggcgttcaa gccgtcggac gtcctctcat
     4501 ctttgacttc gtcatcgact gttatccggc aggtgatcga gccgccgtcg ccctgagcga
     4561 cgatgttcgg cgcggccgac ggcgccgtcg tctgcaa
//
LOCUS       DASYLX010001186         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1528971,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001186 DASYLX010000000
VERSION     DASYLX010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528971"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGP27_28040"
     CDS             <1..801
                     /locus_tag="VGP27_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEV7855182.1"
                     /translation="GETPNPCVRCNERIKFSALSARALALGFDAVATGHYARLTDGRL
                     RRAVDGDKDQSYVLGVLTAEQLRHALFPIGDTPKPQIRQEAARRGLAVADKPDSHDIC
                     FIPSGDTRAFLGSRIGVRRGSVVDGGGTVLAEHDGVHGFTIGQRKGLGIAGPGPDGRP
                     RYVTAIDADTATVRVGALTDLEVWTLVGEKAVFTSGTVPHGPIECAVQVRAHGGIADA
                     VAEVRNGALVADLRAPLLGVAPGQTMVLYRPDADGDEVIGSATIAHRS"
     gene            complement(798..1442)
                     /locus_tag="VGP27_28045"
     CDS             complement(798..1442)
                     /locus_tag="VGP27_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7855183.1"
                     /translation="MSSAAGRLAVLCAATLLAAGCAQTIHGTATRAAPGIDDESHSPV
                     DVESVMLDQGQMRAITGAGDHLTIVPSMDGKIPVDVSDLMNGTPQQCQWYFAETQTFG
                     PEVEEFHKTTFQNPPDGGLISEGAAAYRDPETARHAFADLVGQVYDCGSTELGSTFIG
                     EWTADADTLRMRSAGTCGRDYRIKNVVVAEVSFCSFPDSVPDIVMTNIMDKVPN"
     gene            1536..2552
                     /locus_tag="VGP27_28050"
     CDS             1536..2552
                     /locus_tag="VGP27_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV7855184.1"
                     /translation="MGGFAAGTGIGSWPGTSAREAAEIVVGELHRLPHLVELPARGVG
                     ADLIGRAGALLADINIDTVPRGYRIAAGRSAVTRRAVSLLAEDVDALEEAWETAGLRG
                     SQRTVKVQAVGPITLAAQLELSNGHRAITDPGAVRDLARSLAEGVAAHRAEVARRLDC
                     PVVVQFDEPLLPAALAGRLTGVTSMTPVHPVDESAAGALLDECVTVVGGEVALHSCAA
                     ELPWPLLQRSALSAVSIDLSTLTAGDVDGVGEFVESGRTVLFGVVPAAAPDSRPSARE
                     VAGSVADFTDRLGFPRTVLPERVGITPVCGLAGATPQWARIAIELVQEAADAIAADPA
                     AIMK"
BASE COUNT          398 a          865 c          900 g          419 t
ORIGIN      
        1 ggcgagacgc cgaacccctg tgtgcggtgc aacgagcgga tcaagttctc tgcactgtcg
       61 gcccgtgcgc tcgcgctcgg attcgacgcc gtcgcgaccg gtcactacgc gcggctcacc
      121 gatggtaggt tgcggcgcgc cgtcgacggc gacaaggacc agtcgtatgt gcttggagtg
      181 ctgaccgccg agcagctgcg ccatgcgctg ttccccatcg gcgacacacc caagccgcag
      241 atccggcagg aggccgcgcg ccgcggcctg gccgttgccg acaaacccga cagccacgac
      301 atctgcttca tcccgtccgg ggacaccagg gcgttcctcg gcagccgcat cggggtccgg
      361 cgggggagtg tggtcgacgg cggcggcacg gtgctcgccg aacacgacgg cgtgcacggc
      421 ttcaccatcg gccagcggaa gggcctcggc atcgcaggac ccggccccga cgggcgtccc
      481 cgctatgtga cggccatcga cgccgacacc gcgacggtgc gcgtcggtgc cctcacggac
      541 ctcgaggtgt ggacgctggt gggcgagaaa gccgtcttca cctcaggcac ggtgccgcac
      601 ggacccatcg aatgtgcggt gcaggtgcgc gcacacggcg gcatcgccga cgccgtggcc
      661 gaggtgcgca acggggcttt ggtagccgac ctgcgtgccc cactgctcgg cgtggcgccg
      721 ggccagacca tggtgctcta ccgtccagac gccgacggcg acgaagtcat cggcagcgcc
      781 acgatcgcgc acaggtctta gttgggaacc ttgtccatga tgttcgtcat gacgatgtcg
      841 ggcaccgaat cggggaacga acagaacgac acctcggcca ccacgacgtt cttgatccgg
      901 tagtcgcggc cgcacgtacc cgccgagcgc attctcagcg tgtcggcgtc ggcggtccac
      961 tcgccgatga aggtcgaacc gagttccgtt gaaccgcagt cgtatacctg tccaaccaga
     1021 tcggcgaacg cgtgtcgtgc ggtctcgggg tcgcgatatg cggcggcgcc ttcggagatc
     1081 agcccgccgt cgggcggatt ctggaaagtc gtcttgtgga actcttcgac ctcagggccg
     1141 aacgtctgcg tctcggcgaa gtaccactga cattgctgcg gcgtgccgtt catcagatcg
     1201 ctgacgtcga cggggatctt gccgtccatg ctgggcacga tggtcaggtg gtcgcctgcg
     1261 ccggtgatgg cccgcatctg accctgatcc agcatcaccg attccacgtc gaccggtgaa
     1321 tgcgattcgt cgtcgatacc gggagcggcc cgcgtcgccg tgccgtggat ggtctgtgcg
     1381 cagcccgcgg cgagcagcgt tgcggcgcac aacaccgcca gtcggccggc cgctgaggac
     1441 atggtgtcgg gtccttcctg tttcgcagac taacgcctgt ggcgcgtgag gcgaatagtg
     1501 ttggtgaagt gaccagtaac gatttcgctt cccacgtggg cggcttcgcc gccggcaccg
     1561 gcatcggatc ctggccgggc acgtcggctc gggaggccgc cgaaatcgtg gtcggagagt
     1621 tgcatcgact gccgcatctg gtggagttgc ctgcccgtgg agtcggggcc gacctcatcg
     1681 gacgggccgg cgcactgctc gccgacatca acatcgacac cgtgccgagg ggctaccgca
     1741 tcgccgcagg acgcagtgcg gtgacccgcc gtgcggtcag cctgctcgcc gaggacgtcg
     1801 atgcgcttga agaggcatgg gagacggccg gtcttcgggg gtcgcaacga accgtcaagg
     1861 tacaggcggt gggcccgatc acgctcgcgg cacaactgga actgtccaac ggacaccggg
     1921 cgatcacgga tccgggtgcc gtccgcgacc tggcgcgatc gctggccgaa ggcgtcgcgg
     1981 cgcaccgcgc cgaggtcgcg cgccgactcg attgccccgt cgtggtccag ttcgacgaac
     2041 cgctgctgcc tgcggcgctg gcagggcgac tcaccggtgt gacgagcatg acgccggtgc
     2101 acccggtcga cgaatcggcg gctggggcgc tgctggacga gtgcgtgaca gtcgtcggcg
     2161 gcgaagtggc cctgcacagc tgtgcggccg aactgccctg gccgctattg cagcgcagtg
     2221 ctctcagcgc ggtctcgatc gacctgtcga cgctgacggc aggcgacgtc gacggcgtgg
     2281 gcgagttcgt cgaatcgggc cgcaccgtgt tgttcggcgt cgtacccgcg gccgcacccg
     2341 actcccgccc gtcggcgcgg gaggtggccg gttcggtggc cgatttcacc gaccgactcg
     2401 ggttcccccg cacggtgctg cccgagcggg tcggcatcac cccggtatgc ggcctggccg
     2461 gcgcgactcc gcaatgggcc cgcatcgcga tcgaactcgt ccaggaagcc gccgacgcca
     2521 tcgccgccga cccggccgcg atcatgaaat agcgtttgcc gggcaaaggt ttgtgtaaca
     2581 at
//
LOCUS       DASYLX010001187         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3283767,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001187 DASYLX010000000
VERSION     DASYLX010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283767"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1444)
                     /locus_tag="VGP27_28055"
     CDS             complement(230..1444)
                     /locus_tag="VGP27_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_054608956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV7855185.1"
                     /translation="MRDTELFQAALGLGTPWSVSGSRFDAAARRLDIEITFAKGARFA
                     CPTCGAAGCPAYDTEPLTWRHLSFFQHEAYLTARVPRVSCQACGIRQVSVPWARAGSG
                     FTLLFEAFVMALVASMPVAVVARTVGEHDTKLWRVVHHYVEQARGRADHGEVRRVAFD
                     ETAARRGHNYVSLFVDLDRTRVLFVADGKDAATVGAFAADLQEHGGDAAKVAEVCIDM
                     SPAFIKGTAEHLPQAAITFDKFHAVKIVNDAVDQVRREEQPGRPELKRTRYAWLKNTT
                     NLSSGQRDTVESLSRRNLKTARAWQIRLTFQEVYQQPSRQAAEALLKRWYFWATHSRL
                     PPMIDAARTIKRHWDGILRWHDTHIANGVLEGINSLVQAAKAKARGYRSTRNLKAIIY
                     LIAGKLELSLPT"
BASE COUNT          336 a          626 c          645 g          329 t
ORIGIN      
        1 cttcatgctg aggcggatga cgggcaggat gtcgccatcc tggtccgagc ggatgcataa
       61 gccagcacgc cggacggcgg tgacccagcg gtgtgggtcg tcacatgatg acgccgacca
      121 cagccgcgcg acccgctgga acagcgcggc tcgctcttct cttactccag catgaagatt
      181 ttcccgggca agccaccatc acagtcgaag tccgaggctc ctcgctgttt caagtgggta
      241 gactgagttc gagcttgccg gcgatgaggt agatgatcgc cttgaggttg cgggtggatc
      301 ggtagcctcg agccttggcc ttggcggcct ggacgaggct gttgatgccc tcgaggacgc
      361 cattggcgat gtgggtgtcg tgccagcgca ggatgccatc ccagtggcgc ttgatggtcc
      421 tggcggcgtc gatcatcggc ggcagacggc tgtgggtggc ccagaagtac caccgcttga
      481 gaagcgcctc cgccgcctgg cgtgacggct gctggtagac ctcctggaag gtcaggcgga
      541 tctgccaggc acgggcggtc ttgagattgc gccggctcag gctctcgacg gtgtcgcgct
      601 ggcctgacga gaggttggtg gtgttcttga gccaggcata gcgggttcgc ttcagctccg
      661 gccgccccgg ctgttcctcg cggcggacct gatcgacggc atcgttgacg atcttgacgg
      721 cgtggaactt gtcgaaggtg atcgcggcct gcggcaggtg ctcggcggtg cccttgatga
      781 aggccgggct catgtcgatg cagacctcgg cgaccttggc cgcatcgccg ccatgctcct
      841 gcaggtcagc ggcgaaggcg ccgaccgtgg cggcatcctt gccgtcagcc acgaacaaca
      901 cccgcgtgcg atcgaggtca acgaagagcg agacgtagtt gtggccgcgg cgtgcggcag
      961 tctcatcgaa ggcaacccga cgaacctcgc catgatcggc gcgaccccgg gcctgctcga
     1021 catagtggtg gacgacccgc cacagcttgg tgtcgtgctc gccgaccgtg cgggccacca
     1081 cagcgaccgg catgctggca accagggcca tgacgaaggc ttcgaacagc agcgtgaagc
     1141 cgctgccggc ccgggcccat ggcacgctca cctgccggat cccgcaggcc tggcagctga
     1201 cccgcggcac ccgcgccgtc aaataggcct cgtgctggaa gaagctcagg tgccgccagg
     1261 tcagcggctc agtgtcatag gccgggcatc ccgccgcgcc gcaggtgggg caggcgaacc
     1321 gcgcgccctt ggcaaaggtg atctcaatgt ccagtcgccg cgccgccgca tcgaagcggc
     1381 ttccggacac cgaccacggc gtccccaacc ccaaggccgc ctgaaacaac tcggtgtcgc
     1441 gcatctgcgt ctcgctgcct gatccgctct cagacatgcc agatgaggcg ccccaaacaa
     1501 acccgcacgc gccaccccgg gcacgctggg tgaacgttca atcggccgcc caggcaaaag
     1561 ccgagcgcaa cttgtgcgtg cggggctacc cattccagac agcgaggagc ccaaaggtgc
     1621 ttatgtggac ggcccttggc aggtcaatat gaccgtacgg cgagtgggcc gccagacgaa
     1681 cgtgaacgtt cttcagtctg tcacaccaga acgcagctcc ttgttcgacg ccgccctgcg
     1741 cgccgtggca catgtcctgt cagcagttgg catcaacggt ctccgcacga tgcggcttca
     1801 tcgcgtgctc gaccagctgc tgtcgatcac gatgcgcagg ccgtgctggt ctccgatggg
     1861 gtgcggcacg gttcgatgag acaggcggct gcggtagaaa tcaccgcaac cggaggtcag
     1921 agccatggat cagatc
//
LOCUS       DASYLX010001188         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1858782,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001188 DASYLX010000000
VERSION     DASYLX010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858782"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGP27_28060"
     CDS             complement(<1..191)
                     /locus_tag="VGP27_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEV7855186.1"
                     /translation="MLEQIRGPADLQHLTQSQLTDLAYEIRQFLIHKVAATGGHLGPN
                     LGVVEVTLALHRVFDSPHDP"
     gene            complement(220..1518)
                     /gene="nhaA"
                     /locus_tag="VGP27_28065"
     CDS             complement(220..1518)
                     /gene="nhaA"
                     /locus_tag="VGP27_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEV7855187.1"
                     /translation="MSDQSGPLPRRLLTRGSWPEAKRLAEILRMETVGGVLLLVAAGA
                     AIAWANSPWSDAYHALSDFAIGPQSLHLRLSVAEWAADGLLAIFFFVVGLELKREFVA
                     GDLRDPARAALPIAAAVGGMVVPAAVFVAINVGTGHRENVDGWAVPVATDIAFALAVL
                     AVLSSHLPSALRTFLLTLAVMDDLLAITIIAAFYTEELAPRPLVLAVVPLALFGLAVQ
                     RGYSRWWILLPLAAATWALVHASGVHATVAGVLLGFLVPVSSPRDQLTAESLEHRLRP
                     VSAGFVVPLFAFFAAGVTVGGWSGLGASLLKPITVGVLAGLVLGKPIGVFGTTFVLAK
                     FTRASLDDDLAWRDVFGVSLLAGIGFTVSLLIGELAFGHGTAPGVDAKMGVLSGSVVA
                     ALLAAAVLLSRNAAYRRIHATETLDADHDGVPDVYDTRRD"
BASE COUNT          354 a          566 c          575 g          239 t
ORIGIN      
        1 gggtcgtgcg gcgagtcgaa cacccggtgc agggcgagcg tgacctcgac gacgcccagg
       61 ttggggccga gatgtccacc ggttgcagcg accttgtgga tcaggaattg acggatctcg
      121 tatgccagat cggttagctg agactgggtc aggtgctgca gatcggcggg gccgcggatc
      181 tgttcaagca ttccgtcagt ctacgcatcg atataggcgt cagtcgcgtc gagtgtcata
      241 gacatcgggc acaccgtcgt ggtcggcgtc gagcgtctcg gtggcgtgaa tccgccggta
      301 ggcggcgttg cggctgagca aaacggcagc ggccagcaga gctgcgacaa cggacccgct
      361 cagcacaccc attttggcgt cgacaccggg agctgtgccg tgaccgaagg ccagctcacc
      421 gatcaataac gaaactgtga accctatccc cgccagcagt gagaccccga acacgtcgcg
      481 ccacgcgaga tcatcgtcga ggctggcacg ggtgaacttg gccaatacaa acgtggtgcc
      541 gaacactccg atcggtttgc caaggacgag gccggctagc acaccgacgg tgatcggttt
      601 cagcagcgac gcaccgagac cggaccaccc gccgacggtc actcctgccg cgaagaatgc
      661 gaacaatggc accacgaagc ctgcggatac cggccgcaac cggtgctcca gcgactccgc
      721 ggtgagttga tcccgcgggc tggaaacggg gacgaggaaa ccgagtagga cgcccgcgac
      781 ggtggcgtgc accccgctcg cgtgcaccag cgcccacgtt gccgcggcca atggcaacaa
      841 gatccaccaa cggctgtatc cgcgctgcac cgccaacccg aacaacgcaa gcggaaccac
      901 cgcgagcacc agcggccgcg gcgcgagctc ctcggtgtag aacgccgcga tgatggtgat
      961 cgccagcaaa tcatccatta cggccagagt cagcaggaag gtgcgcagcg cgctgggcag
     1021 gtgtgaggac agcaccgcca gcaccgccaa tgcgaacgcg atatcggtgg cgaccggcac
     1081 ggcccagccg tcgacgttct cccggtggcc ggtgccgaca ttgattgcca cgaagaccgc
     1141 tgcggggacg accattccgc cgacggcggc cgcgatcggc agggcggcgc gcgcagggtc
     1201 gcgaaggtca ccggcgacga attcacgttt gagctccaac cccaccacga agaagaagat
     1261 cgccagcagt ccgtcggctg cccactccgc gacgctgaga cgcagatgca atgactgcgg
     1321 gccgatcgcg aaatccgaga gcgcgtgata ggcgtcggac cacggcgagt tcgcccaggc
     1381 gatcgctgct cctgcggcga cgagcagcag gacaccgccg acggtctcca tgcgaaggat
     1441 ctccgcgagg cgcttggcct ccggccacga cccacgcgtc agcagcctgc gcggaagcgg
     1501 accggattga tcactcatcg cgtgctcctc gggcggccga catcgacgcc gaccagactt
     1561 cccggcacac ctggaggtca acgtagcacc cctaatccgg tcgtgcccgg atagcgtcga
     1621 ggcgatgatc gtcgagcggc agagcatcgt aggcgcacca acggagcgag tgtgggcgcg
     1681 cgtggtgacg ccggagggca tcaacgacga actgcgcccg tggatgacga tgtc
//
LOCUS       DASYLX010001189        12075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3506204,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001189 DASYLX010000000
VERSION     DASYLX010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3506204"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGP27_28070"
     CDS             complement(<1..1049)
                     /locus_tag="VGP27_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7855188.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGDPVVVANSEGSRTTPSVVAFAR
                     NGEVLVGQPAKNQAVTNVDRTIRSVKREIGTDWSVEIDDKKYTSQEISARILQKLKRD
                     AEGYLGEDITDAVITVPAYFNDAQRQATKEAGQIAGLNVLRIVNEPTAAALAYGLDKG
                     QKEQTILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDDRIVEWLVDKFKG
                     TSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRA
                     EFQRITQDLLDRTRQPFQSVIKDAGISVSEIDHVVLVGGSTRMPAVSELVKEMTGGKE
                     PNKGVNPDEVVAVGAA"
     gene            1237..1947
                     /locus_tag="VGP27_28075"
     CDS             1237..1947
                     /locus_tag="VGP27_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7855189.1"
                     /translation="MNTTVDEIGLGIFRLSTWIPDITEHGFTMNQFLLTGEQPFLFHC
                     GHRQLFPLVSAAVATVIPLGNLRWISFGHVEADECGSVNMWLAVAPHAEVIHGPLACA
                     ISLTDICDRPPVVATDEPLDTGGHRLRFIPTPHVPHNWEAGLWHDEATATLLAGDLFT
                     HVGKCPALTGSDCVAPAMAAEEVFHATGLTTNLTPTLEHLAQLNPTTLAIMHGSSFSG
                     DGANQLRGLADGYSAMVR"
     gene            1992..2510
                     /locus_tag="VGP27_28080"
     CDS             1992..2510
                     /locus_tag="VGP27_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855190.1"
                     /translation="MLVLSGSLASCHSATEDNPVTSPGSGTEPSFPTSKPSTSRPTAA
                     PPTKTTPSRPVSPPAAEVLPPDDSGYVFIETKSGQTRCQIDEQSVGCEALFTNSPLQN
                     GVRANGVNLSAGGDMQWIVGNLGDIPAVTIDYRTYSAVGWTIDATHDGTRFTNQRTKH
                     GMFVSIDKVESF"
     gene            3014..3388
                     /locus_tag="VGP27_28085"
     CDS             3014..3388
                     /locus_tag="VGP27_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855191.1"
                     /translation="MSQSLDAVTEWLKAQVHRLLEGARDGGEVLKNKIGEWMDTLADE
                     VADGGPGVNAALGGIRGVLTGQNPMWAAVKGLVSGLSGKAKVALVLLVTLGLLLGPVL
                     LLVLLLALLVAALVAMVRSGSQ"
     gene            complement(3400..3681)
                     /locus_tag="VGP27_28090"
     CDS             complement(3400..3681)
                     /locus_tag="VGP27_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855192.1"
                     /translation="MRLLRGLLGALLWILASVLGLVGLVLCVTIVLLPVGIPLLGVAR
                     RLLTSAIQLMLPRSLAHPVNEVTKTVKKTGRKTGKTLHKVARKSDKLLA"
     gene            complement(3708..3917)
                     /locus_tag="VGP27_28095"
     CDS             complement(3708..3917)
                     /locus_tag="VGP27_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855193.1"
                     /translation="MRSLHGLLSVVLGIIGAAAGAVLSVVASVVWLLGAILCVTVLLI
                     PLGMPVLKLGRRLFTLAGDLMHLGD"
     gene            complement(4140..5099)
                     /locus_tag="VGP27_28100"
     CDS             complement(4140..5099)
                     /locus_tag="VGP27_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7855194.1"
                     /translation="MPLVTASMVCVLVSGPLLLAVTALAGLMMKSTRPVRSAALAMAY
                     ACIELRTLFKLATGDLDGDGLAQEFLTAAYTAARRILDVEVLLDPASATPDIVPRHEP
                     VIVLSRHCGPGDSVLVAWLLTTQYRLQVRAVLKAILRFEPVLDYAGQLGCLCFIARGG
                     RARKQIQDLAASLSGGQALLLFPEGANFSWPRWRAAIAELRSTGRIRAARRALRQSHT
                     LPPHTGGVTAAVTGAPGAHVLVLTHNGFCSDGRARPWWQLPVHRKLLVRTALVPAEHL
                     LQTDQLGTWLEQTWTQVDAWVAGHTDQSDPRMDSHMGLDGAVN"
     gene            complement(5123..5950)
                     /locus_tag="VGP27_28105"
     CDS             complement(5123..5950)
                     /locus_tag="VGP27_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV7855195.1"
                     /translation="MTCNAFVLGGGGVLGAAEAGMARALLEGGVRPDLVCGTSIGAIN
                     GAAIAADPTPGGVERLLSMWDALAEDSLLDGSVFRRVADVLRHRTSLHGNRALRQMLV
                     DRLPAQTFEDLPVPFECVAASIERAREHWFSTGDLIDPVLASCALPGVFPPVRIGDEH
                     FVDGGLVNSIPLDRAVARGADTVWVLHVGRMEEELTAPRFLWEAAFVAFEISRRHRFH
                     ADLERVGSDITVHVLPTGLPARPAATWSNLRYRDTGRIARRADLAYDATRAYLEALP"
     gene            complement(5961..6251)
                     /locus_tag="VGP27_28110"
     CDS             complement(5961..6251)
                     /locus_tag="VGP27_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HEV7855196.1"
                     /translation="MTASGGRIEMDPEEVGRDLGRLVLTIVELLRQLMERQALRRVDA
                     GDLSEEAVERLGVGLMRLEDAMAELRDYFGLRPEDLNLDLGPLGQLLPSQPG"
     gene            complement(6248..6421)
                     /locus_tag="VGP27_28115"
     CDS             complement(6248..6421)
                     /locus_tag="VGP27_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEV7855197.1"
                     /translation="MTLATNATEDVALVDLLDRLLGGGVVIVGDLTISLADVDLVHIS
                     LRALISSVDKLMQ"
     gene            complement(6418..7185)
                     /locus_tag="VGP27_28120"
     CDS             complement(6418..7185)
                     /locus_tag="VGP27_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEV7855198.1"
                     /translation="MSDHNVYLYAIGDSALADPQALAEVRGVDDVPVRTIVQGALAAV
                     VASVDRKRFSENVIREHMEDLEWLEKLARMHHEVVDRIAQNHPIAPVRMATVYLGDAG
                     VRELLDDRHAELAATLDRVRSRSEWGVKGYATPADDASEEEPDSSSDAPGTAYLLRRR
                     AQRDRTARRRQDLLEAAEVIHSQLAAVAGESRRYPLQDARLSGRPEPMILNAAYLVDD
                     AEAVQFEERVHAMSRVDLRLELTGPWAPYSFAAKVDS"
     gene            complement(7178..7570)
                     /locus_tag="VGP27_28125"
     CDS             complement(7178..7570)
                     /locus_tag="VGP27_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEV7855199.1"
                     /translation="MNGDLYPSGRSHQLDHRQESTNLADILERVLDKGIVIAGDIQVN
                     LLDIELLTIKIRLLVASVDKAKEMGIDWWEHDPSLSSGGGDLAVENRRLRERIGELEA
                     SNGDGRGSSQTDGNRRKSRRTEIVDADE"
     gene            complement(7560..7811)
                     /locus_tag="VGP27_28130"
     CDS             complement(7560..7811)
                     /locus_tag="VGP27_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016693990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEV7855200.1"
                     /translation="MARAAMLQIADLTGHDVEGVISVEREDNGWAVGVEVVESHRIPD
                     SADILATYEVHLDKNGELSSYRRTKRYLRGQTFGGRGER"
     gene            complement(7942..8172)
                     /locus_tag="VGP27_28135"
     CDS             complement(7942..8172)
                     /locus_tag="VGP27_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HEV7855201.1"
                     /translation="MGFFTGLLTLPLAPVRGVVWLAEQIQDYAEEQYYDPANIRAELE
                     RVEEARQSGGMTEQECAAWEDELLRRLIHRQE"
     gene            complement(8176..8904)
                     /locus_tag="VGP27_28140"
     CDS             complement(8176..8904)
                     /locus_tag="VGP27_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEV7855202.1"
                     /translation="MTATYIYGVVKADAELPSGLDGLGPGRVGTIAHQRVAAIVSDVP
                     TDRPLGTRDDLIAHERVLDAVAERTVVLPMRFPAVVEEHGVVSELLAPHQERFVEELA
                     RLDGSVQFTVKGRFEQEPVLREIAEADDEIMALRERVRGLPEDATYYDRIRLGELVVT
                     AMQQRRDEVGTRVLNRLEPFVVDISPRRLAQPDEVVNAAFLVHRDRIEHFDDAVEAVG
                     RELAGRVRFRLVGPLAPYDFVQEG"
     gene            complement(8904..9362)
                     /gene="gvpJ"
                     /locus_tag="VGP27_28145"
     CDS             complement(8904..9362)
                     /gene="gvpJ"
                     /locus_tag="VGP27_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEV7855203.1"
                     /translation="MTTQRSPAGQVERYTGGGPSGLAEVIELILDKGLVIDVFVRVSL
                     VGIEILTIDARIVVASVDTFLRFAEATNRLDLYAHGGKDLGDLAKSVTEGGAKGKTSG
                     AIEGAVEKVGQVFTKGRRGDDDEADLEDEEESEPEPAPRRRPRPRARERR"
     gene            complement(9365..10615)
                     /locus_tag="VGP27_28150"
     CDS             complement(9365..10615)
                     /locus_tag="VGP27_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7855204.1"
                     /translation="MASTSTNGESKGRGGTATDTLRGAGQDLLGVVLQRAASSASDRI
                     NGFADRLTDVSETGEGLTSLVRRPRSHDDDDDGDDGDGEDGGGGGGLLGGLKDKVKDV
                     FGGGGKGGGGGGKKLKLTNIVETLDVGLPLRTTYDLWTQYTDFPTFMKKLEAVDQSSD
                     EKTHWKAQVWWSHREWEATTVEQVPDSHIVWRSKGAKGHVDGAVSFTALGPNMTRVAL
                     VIEYFPQGLFERTGNLWRAQGRRIRLEFKHFRRHAMTQALLHPEEIEGWRGEIRDSEV
                     VKTHEEALEEEQRDSGEPEDEQDYPEASAEDEPDEDVDDDVEDEDLGDEDYDTEAVDD
                     DDLDDAEDYADEPEEEDAYDEEPDEEDAYDDEGDDDAGGEEDYDDDYEDEDFEDDGET
                     PSRRPRRRGRQPVGAGRGRGGGRG"
BASE COUNT         2106 a         4103 c         3660 g         2206 t
ORIGIN      
        1 gcagcgccga cggccacgac ctcgtccggg ttgacgccct tgttgggctc cttgccaccg
       61 gtcatctcct tgaccagttc ggagaccgca ggcatacggg tggagccacc gacgagcacc
      121 acgtggtcga tctcggagac cgagatgccg gcgtccttga tgacggactg gaacggctgc
      181 cgggtgcgat ccagcagatc ttgcgtgatg cgctggaact cggcacgggt cagctgctcg
      241 tcgaggaaga gcgggttctt gtccgcgtcg accgtgatgt agggcaggtt gatcgaggtg
      301 ctctgcgagc tggacagctc gatcttcgcc ttctcggcgg cctcacgaag ccgctgcatg
      361 gccatcttgt ccttggtcag gtcgatgcca ctggtgccct tgaacttgtc gacgagccac
      421 tcgacgatcc ggtcgtccca gtcgtcgcca ccgaggtggt tgtcaccgga ggtggcacgg
      481 acttccacga cgccttcgcc gatttccagc agcgagacgt cgaaggtgcc gccaccgagg
      541 tcgaacacca ggatggtctg ttccttctgg cccttgtcca gaccgtaggc cagtgccgcc
      601 gcggtcggtt cgttgacgat gcgcagcacg ttgagcccgg cgatctggcc ggcctccttg
      661 gtcgcctggc gctgggcgtc gttgaagtag gcggggacgg tgatcaccgc atcggtgatg
      721 tcctcgccga gatagccttc agcgtccctc ttcagcttct gaagaattcg cgcgctgatt
      781 tcctgcgagg tgtacttctt gtcgtcgatc tccaccgacc agtcggtgcc gatctcacgc
      841 ttgaccgaac ggattgtccg gtcgacgttc gtcaccgcct ggttcttggc gggctgaccg
      901 accagcacct cgccgttgcg ggcgaaggcc acgaccgacg gggtggtgcg ggagccctcg
      961 gagttggcga cgacgacagg gtcgccaccc tccagtaccg cgacgacgga gttggtggtc
     1021 ccgaggtcga ttccgaccgc acgagccatg gttttcctcc tgaattgctt tgttagggtc
     1081 tgagtggacc gcgctcaagc ctgctactgc cgacgcttcc gtgtcaagcc agacttgagc
     1141 ctggtccact caggttatca tttgcgtcaa cggcgggtcc gcgcggattg ttccggattc
     1201 cgcaaagttc cccggaggtg ggcggacgat agtcggatga acaccacagt cgatgaaata
     1261 gggctgggca tcttcaggct gtctacctgg ataccggaca tcaccgagca cggcttcacc
     1321 atgaaccagt ttctgctcac tggagagcag ccgttcctgt ttcactgcgg ccatcggcag
     1381 ctgttcccgc tggtgtctgc ggccgtcgcc acggtcattc cactgggaaa cttgcgctgg
     1441 atctcgttcg gccacgtcga agctgacgaa tgcggatcgg tgaatatgtg gctggccgta
     1501 gcgccgcacg ctgaagtgat tcacggtccg ctggcgtgtg ccatctcgct gaccgatata
     1561 tgcgaccgac cgcccgtggt ggcaaccgac gagccgctcg acaccggcgg acaccgtttg
     1621 cgcttcatcc ccaccccgca cgttccgcac aactgggagg cggggctctg gcatgacgag
     1681 gcaaccgcca cactgctcgc cggagacctg ttcacacatg tgggcaagtg ccctgctttg
     1741 accggatccg attgtgtggc gccggcgatg gcggccgagg aggtcttcca cgcgaccggg
     1801 ttgacgacga acctgacccc gacgctcgag catctcgcgc agctgaaccc cacaacgctg
     1861 gcgatcatgc acggctcgtc gttcagcggc gacggtgcca accaactgcg cgggttggcg
     1921 gacgggtact ccgcgatggt tcgataacac ccgtgagcac acgtgtagcg cggctcgcga
     1981 ccgccggcgt cgtgctcgtg ttgagcgggt ccctggccag ctgccattcc gctactgagg
     2041 acaacccggt aaccagcccc ggctccggca ccgagccgag ctttcccacc tcgaagccca
     2101 gcaccagcag gccgacggcg gcgccgccca cgaaaaccac accctcgcgg ccggtatcgc
     2161 cgcccgccgc ggaggtgctt ccgcccgacg actccggcta tgtcttcatc gagacgaagt
     2221 cgggccagac ccgctgccag atcgacgagc agtcggtcgg ctgcgaggcc ctcttcacca
     2281 attcgccgct gcaaaacggt gtgcgggcca acggcgtgaa cctgtccgcg ggcggtgaca
     2341 tgcagtggat cgtcggcaac ctcggcgaca tcccggccgt caccatcgat taccgcacct
     2401 acagcgccgt cggctggacg atcgacgcca cccacgacgg cacccggttc accaaccagc
     2461 gcacgaagca cggcatgttc gtcagcatcg acaaggtcga atcgttctag gcgctcctca
     2521 gctggctccg ttggggtttc gagaattgac cgtggggtaa ttatctctca tgagtgggca
     2581 aaacacgccg aacagccggc ctgaaccgac cagcttgcgc accagacgtg cgtccggcct
     2641 cagccggaat cctgggaaga cgacgaggac tgcgacaggt agaaaatcgt cgaaagggcc
     2701 gaagtctgtg tccgaagccg tggtggtaca gcttccgcgc agacgtgcgg cggccacgag
     2761 gtcgcggggc acgacgcatc gcagcgcagc gaagcgccgc aatgcatcag gcacgtccgg
     2821 cttgagtgca caccggcagc ggccggtcga cgacatcacg cccccggcac tcgcgcaggc
     2881 ccaggccagc actgccgcac cgacggctcg caagcgttcg cgtgcggaaa cgccccgcgc
     2941 ctctgcaccc tcgcgagctg cctcgtcgaa ttcgggacac cgcccgggga cttcgtccgc
     3001 cggcggcggc ctggtgtcgc agtcattgga tgccgtcacc gaatggctga aagcgcaggt
     3061 tcaccgactc ctcgagggag cgcgggatgg cggggaggtg ctgaagaaca agatcgggga
     3121 gtggatggac actctcgcgg atgaggttgc cgacggcggt cccggcgtca acgcggcgtt
     3181 aggcggcatt cggggtgttc tgacaggtca gaatccgatg tgggccgctg tcaagggcct
     3241 tgtgtccgga ctcagtggca aggccaaggt ggccctcgtt ctcctcgtga ccctcggact
     3301 actgctagga ccggttctgt tgctggtcct actgttggcg ctcctggtcg cagcgctagt
     3361 cgcgatggtg cggtccggat cgcaatgact tgttgagcgt caggccagca gcttgtcgct
     3421 cttgcgcgcc accttgtgaa gcgtcttgcc cgtctttctg cccgtcttct tgaccgtctt
     3481 cgtcacctcg ttgaccggat gggccaacga gcggggaagc atgagctgaa tggcagaagt
     3541 caaaaggcgg cgggcgacac ccaggagggg tatacccaca ggcaagagca cgatggtgac
     3601 gcacagcacc agaccaacga gcccgagcac cgaagcaaga atccagagca gggcgcctag
     3661 cagccctcgt aacagtcgca tcgtaaacct cctcggctcg tcgcgagtca gtcgcctaaa
     3721 tgcatcaggt caccggcgag cgtgaatagc cgacggccga gcttcaagac aggcataccc
     3781 agcggaatca ggagcaccgt cacgcacaga atcgcgccga gcagccacac cacgctggct
     3841 accactgaca acaccgcgcc cgcggcggcg ccgataatcc cgagcaccac gctgagcagt
     3901 ccatgtagcg atctcatgat catccctctc actccaacgg actagagctt gcgaagcgcc
     3961 tccgaggggt tccatcgctc ctgcacagtc tcgccgaatt ggtcgaaaga gatcacttcc
     4021 acggctcggc aagaggcaca caaacccgaa ccccagaccg ggtaaccgca tccacagcaa
     4081 acattgatca cggtcgcccc gttcgcgcct cgcatgccat ttggctaccc acttcagagt
     4141 taattaaccg caccgtcaag gcccatatga ctgtccattc gtgggtccga ttggtcggtg
     4201 tggccggcga cccaggcgtc tacctgcgtc caggtctgtt cgagccatgt gcccaactga
     4261 tcggtttgga gtaggtgctc ggccggcacc aacgcggtgc ggacaagcaa ttttcgatgg
     4321 accggtagct gccaccatgg ccgtgcccgc ccgtccgagc aaaaaccatt gtgggtcagc
     4381 accagcacat gggcgcccgg cgcgcccgtc acggcggcgg tgacacctcc tgtgtgcggg
     4441 ggaagcgtat gagactgacg taaagcgcgt ctcgcggcgc ggatccggcc ggtcgagcgc
     4501 agttcggcga tggcggcgcg ccagcggggc caactgaaat tcgccccctc gggaaagagc
     4561 agaagcgcct ggccaccgga cagcgacgct gcaaggtcct ggatctgctt gcgggcccgg
     4621 ccgcctcgcg cgataaaaca caggcacccg agctgccccg cgtagtcaag caccggctcg
     4681 aagcgcagaa tcgccttgag cactgcccgg acctgtaacc ggtactgggt tgttaggagc
     4741 caggcaacca gcacgctatc gccgggacca caatgccgcg acagcacgat caccggctcg
     4801 tggcggggaa caatgtccgg ggttgccgag gccgggtcaa gcaagacctc aacatcaagg
     4861 atccgccgcg ctgcggtgta cgccgctgta aggaactcct gcgcgagccc atcaccgtcc
     4921 agatcgccgg tcgcgagctt gaaaagcgtg cgaagttcga tgcaggcgta cgccatcgcc
     4981 aaggccgcgc tacgcaccgg ccgggtcgat ttcatcatca gaccggccag cgcggtcacc
     5041 gccaacagca acggccccga aaccagcacg cacaccatgg aggcagtgac cagcggcact
     5101 gtcacaactc gtcgcgacgc aatcatggca acgcctcgag atatgcgcgt gttgcgtcgt
     5161 aggccagatc ggcgcgccgc gcaatgcggc cggtgtcgcg ataccgcaga ttcgaccacg
     5221 ttgcggcggg acgcgccggc aagccggtgg gaagcacgtg cacagtgata tcggaaccca
     5281 cgcgttccag atcggcgtgg aaccggtgcc tgcgggaaat ctcgaatgcc acgaacgcgg
     5341 cctcccacag gaaccgcggg gcggtcagct cttcctccat acgtcctacg tgcaggaccc
     5401 acacggtatc ggcgccacgg gctaccgcgc gatccagcgg aatgctgttc accaggccgc
     5461 cgtcgacgaa atgttcgtca ccgatgcgca ccggtgggaa gacgccaggc agggcgcacg
     5521 acgcgagcac cgggtcgatg aggtcccccg tgctgaacca gtgctccctc gcgcgttcga
     5581 tgctggccgc gacgcactcg aacggcacag ggagatcctc gaaggtctgc gccggcaacc
     5641 ggtcgaccaa catttgacga agcgcgcggt tgccgtgcag tgacgtccgg tggcgcagta
     5701 cgtcggcgac gcgccggaaa accgaaccgt cgaggagact gtcctcagca agcgcgtccc
     5761 acatcgacag caggcgctcg acacccccgg gagtgggatc tgcggcgatc gccgcgccgt
     5821 tgatcgcgcc tattgacgtg ccgcacacca ggtctggacg cacgccccct tccagcagag
     5881 ctcgcgccat gccggcctcg gcggccccga gcacgccgcc gccgcccagc acgaacgcgt
     5941 tgcaggtcat gtgcgcagga tcagccaggc tggcttggca ggagctgacc cagtggcccg
     6001 agatcgaggt tgaggtcctc cggcctaagc ccgaagtagt cccgcagttc cgccatcgcg
     6061 tcctccaacc gcatcagccc gacgccaagc cgctcgactg cttcctcaga caggtcgccg
     6121 gcatccaccc ggcgcagcgc ctgtcgctcc atcaactggc gcagcagttc gacgatcgta
     6181 agcaccagtc ggccgaggtc tcggccgact tcctcgggat ccatctcgat ccggccacct
     6241 gaggcggtca ttgcatcaac ttgtccacgg aactgatcag cgcgcgcagg gagatgtgaa
     6301 ccaagtcgac atcggccaga ctgatcgtca gatcacccac aatcaccacg ccgccgccga
     6361 gcagacggtc gagcagatcg acaagtgcca catcttcggt ggcgttcgtc gcaagagtca
     6421 tgaatcaacc ttcgctgcaa aggaatacgg cgcccacgga cccgtcaact ccagccggag
     6481 atcgacgcgc gacatggcgt gcacccgctc ttcgaattgc accgcttcgg catcgtcgac
     6541 caggtaagcg gcattgagaa tcatcggctc ggggcgtccg ctgagccggg cgtcctgcaa
     6601 tgggtaccgg cggctctcgc cagcgacggc cgcgagctga ctgtgtatga cctccgctgc
     6661 ttcgagaagg tcctggcgtc gacgtgccgt ccggtcacgc tgggcacggc ggcgcaacaa
     6721 ataagcggtt ccgggtgcgt cagaggagga gtcgggctcc tcctctgagg catcgtcggc
     6781 aggcgtcgca taccctttga ccccccattc cgaacggctg cgcacccggt cgagtgtcgc
     6841 ggccaactca gcgtggcggt catcgagcag ttcgcgaaca cccgcgtcgc ccaggtaaac
     6901 ggtggccatc cgaacagggg caatcggatg gttctgcgct atccggtcca ccacctcatg
     6961 gtgcatcctt gcgagcttct ccagccactc caaatcctcc atgtgctcgc gaatcacgtt
     7021 ctcgctgaag cgtttgcggt ccaccgatgc gaccacggcg gccagcgcgc cctgaacgat
     7081 ggtgcggacc ggaacatcgt cgacgcccct tacctcggcc agtgcctgag gatcggccag
     7141 cgcgctgtca ccgatcgcgt agaggtagac gttgtggtca ctcatccgcg tcgacaattt
     7201 cagtgcggcg actcttacga cggttaccgt cggtctgcga cgatcctcgc ccgtccccgt
     7261 tcgacgcctc tagttcgccg atgcgttccc gcagccggcg gttctcgacc gctaagtcgc
     7321 ctccgcctga gctgagcgac gggtcatgct cccaccagtc gatgcccatc tccttggcct
     7381 tgtcgacgga cgccaccagc agccggatct tgatcgtcag cagctcgatg tccaacaggt
     7441 tcacctggat atcgccggcg atgacgattc ccttgtcgag aacccgctcc agaatgtcag
     7501 cgaggttcgt gctttcctgg cgatggtcga gctggtggga ccgcccagac ggatacaggt
     7561 caccgttcac cgcgaccccc gaacgtctgt ccacgaagat agcgcttggt gcgacggtag
     7621 gacgatagct caccgttttt gtccagatgc acctcgtagg tggccaggat gtcggccgag
     7681 tccggaatgc gatggctctc aaccacttcg acgcctactg cccagccgtt gtcttcacgc
     7741 tccacggaga tcacaccctc cacgtcatgg ccggtgaggt cggcaatctg taacatcgcg
     7801 gcgcgagcca cgttcgccgg ggagagccgg cgcgggtgct ggccgttagt gctgtcagac
     7861 ctcgcgcgtg atgcagtcgg cgccctccgg cgtctcggtc gatcggtctg ctcggccatg
     7921 ttggtccacg agtccttgcg ttcactcctg ccggtggatc aaccggcgaa gtaactcgtc
     7981 ttcccacgcg gcacattcct gctcggtcat cccgcccgat tgccgggctt cctcgacccg
     8041 ttcaagttcg gcgcggatgt tcgccgggtc gtagtactgc tcttctgcgt agtcctgaat
     8101 ctgttccgcc agccacacga cgccgcggac gggtgcgagc ggaagggtca gcagtccggt
     8161 gaaaaacccc acggttcagc cctcctgcac aaagtcgtag ggggccagcg ggccgacgag
     8221 ccggaagcgc acgcggccgg caagttcccg gccgaccgct tccacagcgt catcgaagtg
     8281 ttcgatgcga tcgcggtgca ccaggaacgc agcattcacc acctcgtcag gctgagccag
     8341 ccgacgcggg gagatgtcca cgacgaatgg ctccagccga ttcagcacac gcgtgcccac
     8401 ttcgtcccgc cgctgctgca tcgcggtcac cacgagttca cccaagcgaa tccggtcgta
     8461 gtacgtcgcg tcctccggca ggccacgcac ccgctcgcgc agcgccatga tttcatcgtc
     8521 tgcctctgcg atctcgcgaa gcacaggctc ctgctcgaac cggcctttca cagtgaattg
     8581 aaccgaaccg tccagtcgcg caagttcctc cacaaaccgt tcctggtgcg gggcaaggag
     8641 ttccgacacc accccgtgct cttccacgac ggccgggaaa cgcatcggca gcaccaccgt
     8701 ccgctcggcg actgcatcca gcactcgctc atgtgcgatg aggtcgtcgc gggtgccgag
     8761 cggacggtcg gtgggcacat cgctcacaat cgccgcgacg cgctgatgcg cgattgtgcc
     8821 gactcgaccg ggccccagtc catcgagacc cgacggcagt tcggcgtccg ccttgaccac
     8881 cccgtagatg tacgtagcgg tcattaccgt cgctccctgg cccgcggccg cggacgtcgc
     8941 cgcggcgccg gctccggttc agactcctcc tcgtcttcta ggtcggcctc atcgtcgtcg
     9001 ccgcgccggc ctttggtgaa gacctgtccc accttctcga cggcgccctc gatcgctccg
     9061 gaggtctttc ccttcgcgcc gccctcggtc actgacttcg caaggtctcc caagtccttg
     9121 ccgccgtggg catagaggtc cagccggttg gtcgcctcgg cgaaccgcag gaaggtgtcc
     9181 acgctggcga cgacgattcg cgcatcgatg gtgaggatct caatcccaac cagggaaacc
     9241 cgaacgaaga cgtcgataac gagacccttg tcgaggatga gttcgatgac ctcggccaag
     9301 ccgctgggcc caccgccggt atagcgctcg acttggccgg cgggactccg ttgcgtcgtc
     9361 atagtcatcc gcgcccaccc ccgcgacccc ggccggcgcc gacgggctgc cgtccacgtc
     9421 tacgcggccg acgagaaggt gtttcaccgt cgtcttcgaa atcctcgtcc tcgtagtcgt
     9481 cgtcgtaatc ttcctcgccg cccgcgtcgt cgtcgccctc gtcgtcgtag gcgtcctcct
     9541 cgtcgggctc ttcgtcgtag gcgtcctcct cctcaggctc gtcggcgtag tcctcagcgt
     9601 cgtcgagatc gtcgtcatcg accgcctcgg tgtcgtagtc ctcgtccccg aggtcctcgt
     9661 cttcgacgtc gtcgtctacg tcctcgtcgg gctcgtcttc agcggacgct tccgggtaat
     9721 cctgctcgtc ctcaggctcg ccggaatccc gctgctcttc ctccagtgct tcttcgtgcg
     9781 ttttgactac ctcgctgtca cgaatctcgc cacgccagcc ctcgatctcc tccgggtgca
     9841 acaacgcctg cgtcatcgcg tgcctgcgga agtgcttgaa ctcgaggcgt atgcgccggc
     9901 cctgggcccg ccacagattg cccgtccgct cgaacaaccc ttgcgggaag tactcgataa
     9961 ccagcgccac ccgggtcata ttcggtccaa gcgcagtgaa cgaaacggct ccgtcgacgt
    10021 ggcctttggc acccttggat cgccagacga tatgggaatc cggcacctgc tcgacggtcg
    10081 tggcctccca ctcgcgatgt gaccaccaga cctgggcctt ccaatgggtc ttttcgtccg
    10141 acgactggtc gacggcctcc aacttcttca tgaacgtggg gaagtcggtg tactgcgtcc
    10201 acaggtcata ggtggtgcgc aatggcaggc cgacatcgag tgtttcaacg atgttggtca
    10261 gcttcagctt tttgccgccc ccgccgccgc cttttccacc gccgccgaac acgtccttga
    10321 ccttgtcctt caggcctccc agaagacccc cgcctccgcc gccgtcctcg ccatcgccgt
    10381 cgtcgccgtc atcgtcgtca tcgtgactgc gcggtcgacg aacgagcgat gtcagccctt
    10441 cgccggtctc gctcacatcg gtcagccggt cggcgaagcc gttgatgcga tcgctggccg
    10501 aggaggcggc gcgctggagc acgacgccga gcagatcctg gcctgcgcct cgcaacgtat
    10561 cggtcgccgt tccgccgcgg cccttactct caccattggt actggtgcta gccatcgttc
    10621 acctcccggc gcgtgatgct gcgtgacgcc tttcggctcg ctgcagcctt cttcggtgtc
    10681 cgtgcggacg atgtggcgcg cttcttcgcg ggagctgagc gtgcgctggt cttcttggcg
    10741 gcggacctgc cactcgccgt tttgcgcccg ccagatgtgg aggacccacc ggaccggctg
    10801 ccacttgctg cccggccgcc acttgacgag cggcccccac ttgacgagcg gctgctgccc
    10861 gacgtacggc tcgcggccgc cgatcggccg gtgcccgatc ggcttccagc cccggagcgg
    10921 gatttgcccg cgctacggct cgcgccattg ccagtcgagc gggctttacg cgcgcggcgc
    10981 gccggccgct gctcttcgtc cgcctcatcg tctccgtcgt cgacttcgtc gctgtccaag
    11041 cccaagtcat cgaggtccgc tacccgccgc atattgcggt cggcagttcg ttggatatcg
    11101 gtcaagtcct ccacgctccg acccacccgg tccgtcagcg cctcgacctg acgagtcgcg
    11161 acggccatgg cggcgccttt accggcttcc agtaggcggc ctcgcacttg ttcggacaac
    11221 gccgaaagct ccggcgactc gcccagcagt ttgcctgctt gaccgagaat ctggcctgga
    11281 ccgcctacct ggcgtccggc cgccatgccg ccaagcgcca aggcgagctt catcttcttg
    11341 gtccgtccga ggaagtatcc tccagcgacg ccgagcgcta tacgggctcc acatttcacg
    11401 acatactcct aggctcgatt tgtaatcggg atctcaggtc cggcgatccc attccgacga
    11461 cccgcgtccc atatcgggcg aatacccgga gctgaaaaca ccaaacgctt caatacaaat
    11521 tggttaacct ctttcgtacc ccgcgaactc agtgcgggaa attacccggc aagccgcgtt
    11581 tccgggccgc accgacgaag acatgaatac gtagcgaaga tgccgcaagg gtgccgcggt
    11641 cccgtccgga gatcggtgaa agtgcccgcc agcaggcccg cctctggctg aatcgctcgt
    11701 caggcagcgt tttcatacca cagcggaggt cagccttctg gttgcgtgat ggagagcgct
    11761 gaccagtcgt ggcagtgttt agcaaccaca cgcgcggttc aggaaattcg cctccctttt
    11821 tgcttcatcg gatattcggc cggtatattt tttgcagaat atcgagggct accacattta
    11881 cttaaacgcg tggggcacta cccgtgtgcc gatgatcggc cgttcatccc acccgaaata
    11941 aaagcgcctc aataaattgc cgaagccccg agagtttcgt gctcctccta tgaccgaaat
    12001 ggtgtttcgg ccgtgcaatc atgcggtacg cgaggtggat cgtttgctgt ggtaatagat
    12061 ttacaacagg catcg
//
LOCUS       DASYLX010001190         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_325064,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001190 DASYLX010000000
VERSION     DASYLX010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325064"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VGP27_28155"
     CDS             <1..579
                     /locus_tag="VGP27_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855205.1"
                     /translation="GENPEVKTPVVPPAVSAPAPTPTPTAAVTPPPVLKEGDDCPDST
                     LAVKGITSQPEYVVGDQPKFTMVVTNIGLVACKRDVGAAVLAAYVYGLDNTRLWSNLD
                     CAPSNETLVKTFNPGEQVTTEVTWTGMGSGPGCPLPRQPIGPGTYNLLVQLGNLRSAA
                     MPFILAQPAPQAEATAPADGAPAPQQAPAPAG"
     gene            complement(576..1658)
                     /gene="disA"
                     /locus_tag="VGP27_28160"
     CDS             complement(576..1658)
                     /gene="disA"
                     /locus_tag="VGP27_28160"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEV7855206.1"
                     /translation="MVQLARPTLRETIGRLAPGTALRDGLERILRGRTGALILLGYDD
                     SVESICDGGFALDVGYAPTRLRELSKMDGAVVLSSDGTRILRANVQLVPDPSIPTDES
                     GTRHRSAERAAIQTGYPVISVSHSMSIVTVYVAGERHVVPDSATILSRANQTIDTLER
                     YKSRLDEVSRQLSTAEIEDFVTLRDVMTVVQRLEMVRRISLEIDSDVVELGTDGRQLK
                     LQLEELVGDNDNARELIVRDYHANPDPPTASQVTATLEELDGLSDTELLDFTTLARVF
                     GYPSTAEAQDSAMSSRGYRAMAGIPRLQFAHVDLLVRSFGSLQGLLAASANDLQSVDG
                     IGSMWARHIREGLSQLAESTIADRLA"
     gene            complement(1676..>2550)
                     /locus_tag="VGP27_28165"
     CDS             complement(1676..>2550)
                     /locus_tag="VGP27_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEV7855207.1"
                     /translation="MSTTEADGVTGGVTQVRAVTTALTMTAKTTGIAIILVGHVTKDG
                     AIAGPRSLEHLVDVVLHFEGDKTSSLRMVRGVKNRFGAADEVGCFMLHDNGIECVADP
                     SGLFLDQRPAPVSGTAVTVSLDGKRPLIGEVQALIAAPTTSSPRRAVSGIDSARAAMI
                     AAVLEKRARLRVAQCDIYLSTVGGMRLTEPSADLAVALAIASAYADLPMPATAIAIGE
                     VGLAGDLRRVSGMERRLTEAARLGFTCAVVPPGVKTVPIGLKAIAADNITAAMKVLTE
                     IAVKGGRPEEGSWP"
BASE COUNT          406 a          906 c          834 g          404 t
ORIGIN      
        1 ggggagaacc ccgaggtcaa gacgcccgtc gtgccgccgg ccgttagtgc accggcgccg
       61 acgccgaccc cgaccgccgc cgtcactccg cccccggtgc tgaaggaggg tgacgactgc
      121 ccggacagca cgctggccgt caagggcatc accagccagc cggaatacgt cgtgggcgat
      181 cagccgaagt tcacgatggt cgtcaccaac atcgggttgg tggcctgcaa gcgagacgtc
      241 ggcgccgcgg tgctcgcggc ctacgtgtac ggcctggaca acacccggct gtggtcgaac
      301 ctcgattgcg ccccgtccaa cgagacgctg gtgaagacct tcaacccggg tgaacaggtg
      361 acgaccgagg tgacctggac gggcatgggc tcggggcccg gctgccccct gccgcgtcag
      421 ccgatcggac ccggcacgta caacctgctc gtccagctcg gcaatctgcg gtctgcggcc
      481 atgccgttca tcctggcgca gcccgcgccg caggcagagg ccacggcacc ggcggatggc
      541 gctcctgcgc cccagcaggc gcccgcaccg gccggctagg ccagccggtc ggcgatcgtc
      601 gactcggcaa gctgcgacaa accctcgcgg atgtggcgcg cccacatcga gccgatgccg
      661 tcaacggact gcagatcgtt cgcgctggcg gcgagcaatc cctgcagcga cccgaaggac
      721 cgcaccagca gatcgacgtg agcgaactgc agacgtggga tccccgccat cgcccggtag
      781 ccgcgcgagc tcatcgccga gtcctgtgct tccgccgttg acgggtagcc gaaaaccctt
      841 gccagggttg tgaagtcgag cagctcggta tccgaaagcc cgtctagttc ctcgagcgtc
      901 gcggtcactt gcgatgcggt cggcgggtcc gggttggcgt ggtagtcacg cacgatcagc
      961 tcgcgggcgt tgtcgttgtc gccgaccagc tcctccagct gaagtttcag ttggcggccg
     1021 tcggtgccga gttcgacgac atcggaatcg atttcgaggc tgatcctgcg gaccatctcc
     1081 agtcgctgca ccaccgtcat cacgtcgcgc agcgtgacga agtcctcgat ctcggccgtc
     1141 gacagctgac ggctgacctc gtccagccgc gacttgtacc gctccagcgt gtcgatggtc
     1201 tggttggccc gtgacaagat cgtcgccgag tcgggcacca cgtgccgctc gccggccacg
     1261 tacaccgtca cgatgctcat cgaatggctc accgagataa cgggataccc ggtctggatt
     1321 gcagcgcgct cggcggatcg atgccgggtg ccggactcgt cggtcggaat cgacgggtcg
     1381 ggcaccagct ggacgttcgc ccgcaggatc ctggtgccgt cgctggagag caccacggcg
     1441 ccgtccatct tcgacagctc gcgcagccgg gtcggcgcgt acccgacgtc cagcgcgaag
     1501 ccgccgtcgc agatcgattc gacgctgtcg tcgtaaccga gcaggatcag cgcgccggtg
     1561 cggccgcgca ggatgcgttc caggccgtcg cgcagcgcag ttcccggcgc gagcctgccg
     1621 attgtttccc gcagggtcgg gcgcgccagc tgcaccacgt ttcggcgtct ttccttcacg
     1681 gccatgaccc ctcctcgggg cggccgccct tcaccgcgat ctccgtcaac accttcattg
     1741 cagcggtgat gttgtcggcc gcaatcgcct ttaagcctat cggcaccgtt ttcacaccgg
     1801 gcggaaccac cgcgcaggtg aagccgagcc tggcggcttc ggtgaggcgc cgctccatgc
     1861 cgctgacccg gcgcaggtcg cccgccagac cgacttcgcc gatcgcgatg gccgtcgccg
     1921 gcatgggcag atccgcatag gccgaagcga tcgccaatgc caccgcgagg tccgccgacg
     1981 gctccgtcag ccgcatcccg ccgaccgtcg acaggtagat gtcgcattgc gccaccctca
     2041 gccgggcccg cttctccaac accgccgcga tcatcgccgc gcgcgccgag tcgatgccgc
     2101 tgaccgcgcg gcgcggagac gacgtggtcg gtgccgcgat cagggcctgc acctcgccga
     2161 tgagcgggcg cttgccgtcg agcgacaccg tcaccgcggt gcccgaaacg ggcgccggac
     2221 gctggtcgag gaagaggccc gacggatcgg cgacacactc gattccgttg tcgtgcagca
     2281 tgaaacagcc gacctcatcg gccgcgccga aacgattctt taccccgcgc accatacgaa
     2341 gcgaggacgt tttgtcgccc tcgaagtgca gcactacatc gacgaggtgc tccagcgacc
     2401 gcggaccggc gatcgcgccg tccttggtga cgtgaccgac aaggatgatc gcgattccgg
     2461 tggtcttcgc ggtcatcgtc agcgccgtcg tgaccgctcg cacctgggtg acgccgccgg
     2521 tcacgccgtc ggcctcggtg gtcgacatgg
//
LOCUS       DASYLX010001191         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3177198,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001191 DASYLX010000000
VERSION     DASYLX010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177198"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGP27_28170"
     CDS             complement(<1..618)
                     /locus_tag="VGP27_28170"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139375.1"
                     /note="catalyzes the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEV7855208.1"
                     /translation="MTVLYDNIGELVTNDPAQGDGSALGIHHDAAMVVDGDRIAWLGP
                     KDRAPAADNRVDCRNRCVTPGFVDSHAHLVFAGDRSAEFAARMSGEPYDGGGIATTVS
                     ATRAAGDGELSDNLARLSGELLRNGVTTFETKSGYGLTVADELRSLQIAGRATTETTF
                     LGAHVVPAEYAGRRDDYVSLVAGEMLSACAPHARWIDVFCDRGAFD"
     gene            complement(615..>1734)
                     /locus_tag="VGP27_28175"
     CDS             complement(615..>1734)
                     /locus_tag="VGP27_28175"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEV7855209.1"
                     /translation="WTWRKLMYQAAGQLTPDTYRTLAAAVYAEMALAGITSVGEFHYL
                     HHDIGGRRYADPNEMGKALIAAAADAGVRLTLLDTCYLRSGLDGAPLAEGPQQRFGDG
                     TGEGWADRVQELRSDVGSKRAPQVIIGAAIHSVRAVPPSEMAAVVGWADVNDAPLHVH
                     SSEQTAEVDECMAIHNRTPTELLGDNGVLGPRTTAVHATHLTEQDIAEMDRTATGVCF
                     CPTTERDLGDGIGPAAALLAGRGLFSLGSDSHAVIDLFEEARAVELDERLARRERGIT
                     SAATLLDAATAAGQHVLGWTDAGVLRVGARADLVAVDIRSMRTAGGGPTAENVIFAAS
                     AADVTDVIVDGRTVVTERRHVAYSDIGDALAQSMERIR"
BASE COUNT          259 a          607 c          576 g          292 t
ORIGIN      
        1 atcgaacgcg ccgcgatcgc agaacacgtc gatccaccgc gcatggggcg cacaggcgga
       61 cagcatctcg cccgccacca ggctgacgta gtcgtcgcga cggcccgcgt actcggcggg
      121 aacgacgtgc gcgccgagga aagtggtttc ggtggtcgcg cggcccgcga tctgcagcga
      181 ccgcagttca tcggcgaccg tcaggccgta cccgctcttg gtctcgaacg tagtgacgcc
      241 gtttcgcagg agttcgccgg acaggcgggc gagattgtcc gacagttcgc catcgcccgc
      301 ggcgcgggtc gccgacacag ttgtcgcgat gccgccgccg tcgtagggtt cgccgctcat
      361 ccgtgcggcg aactcggcgg acctgtcacc ggcgaacacc agatgcgcat ggctgtcgac
      421 gaatcccggg gtgacacagc ggtttcggca gtccactctg ttgtcggcgg ccggcgcccg
      481 gtccttcggt ccgagccatg cgatgcggtc accgtcgacg accatcgcgg catcgtggtg
      541 gataccgagg gcagatccgt cgccttgtgc cggatcgttg gtgaccagct cgccgatgtt
      601 gtcgtagaga acggtcatcg gattcgctcc atcgactgcg cgagagcgtc accgatatcg
      661 gagtacgcga cgtggcgcct ttccgtcaca accgttcggc cgtcgacgat cacgtcggtg
      721 acgtcggcgg ccgacgccgc gaagatcacg ttctccgccg tcgggccgcc gcccgcggta
      781 cgcatggacc gaatgtcgac ggcgacgagg tcggcccgcg cgccgaccct cagcacaccg
      841 gcgtccgtcc agcccagcac gtgttgcccc gcggccgtgg cggcgtccag cagtgttgcc
      901 gcggaggtga ttccgcgttc gcgtcgggca aggcgctcgt cgagttcgac ggcgcgcgcc
      961 tcctcgaaaa ggtcgatcac cgcgtggctg tcggagccga ggctgaacag tccccgccct
     1021 gccagcagcg cggccgcagg cccgattcca tcaccgagat cacgttcagt ggtcgggcag
     1081 aagcacacac ccgtcgcggt ccggtccatc tccgcgatgt cctgctcggt caggtgtgtg
     1141 gcgtgcaccg ccgtcgtgcg gggcccgagg actccgttgt cgcccaacag ttcggtaggg
     1201 gtgcggttgt ggatggccat gcattcgtcg acctcggccg tctgctccga ggagtgcaca
     1261 tgcaagggcg cgtcgttgac gtccgcccac ccgacgacgg cggccatctc cgacgggggc
     1321 acagctcgca cggaatgtat tgccgcgccg atgatcacct gcggcgcacg cttcgatccg
     1381 acgtcgcttc tcaactcctg cactcggtcg gcccagccct cccctgtgcc gtcgccgaac
     1441 cgttgttgag gcccctctgc cagcggcgcg ccgtcgagtc cggaacgcag atagcaggtg
     1501 tcgagcagcg tcaggcgcac accggcgtcc gccgccgccg cgatcagcgc cttgcccatc
     1561 tcattcggat ccgcgtagcg cctgcccccg atatcgtggt gcagatagtg gaactcgccc
     1621 acggaagtga ttcccgccaa ggccatctcg gcgtagacgg ccgccgccaa ggtgcggtag
     1681 gtgtccgggg tcaactgtcc cgcggcctgg tacatcagct ttcgccacgt ccag
//
LOCUS       DASYLX010001192         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2846107,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001192 DASYLX010000000
VERSION     DASYLX010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846107"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGP27_28180"
     CDS             <1..287
                     /locus_tag="VGP27_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7855210.1"
                     /translation="RVIAICATPVDDATSDIFSTYWIDEASGNHNERLNAAQAALPDD
                     IAIWEHQIYLDPPGLTTSEAAGFKQLRQWARSFYPDAEAVAAEPTPSGVR"
     gene            301..1044
                     /locus_tag="VGP27_28185"
     CDS             301..1044
                     /locus_tag="VGP27_28185"
                     /inference="COORDINATES: protein motif:HMM:NF028507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEV7855211.1"
                     /translation="MSNAAKYFELVSVVAFVALMVWVVLDTRRRGELGVVGLLAVAGF
                     SIFWQEFYADWGSYLLWTPDFHMLPWGATPWTTPEKPAMNLTSYPVFMTAAFVSMLAL
                     QKWARRRWPGVHPMVLSLITAGPVLIGFNLVMEYVSVKTLGLWTYVDTIGPVLHTDAG
                     TMPLLYPNIPFGLFGAVTAFLIGWTNAEGRPRFEALVAKPGLPQGIKRDVLRTIAWVL
                     TFNVTYWLFLITPAIVARLTLGEPSALVP"
     gene            complement(1095..1553)
                     /locus_tag="VGP27_28190"
     CDS             complement(1095..1553)
                     /locus_tag="VGP27_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855212.1"
                     /translation="MKRAALMGAVGGVLVSVATFIAPPADAANPIGNFDLIIPDRFDF
                     HTWVWSVTPCVAGAAPPDCIHVFGIARPVAKAFNYAGDAALVDGRYTLTVDDPYGLRC
                     RNVYYGPTSTTHDVYSWDATTMAGTLTSSFDTGCDGAPGTLVYPISLSRS"
     gene            complement(1593..2042)
                     /locus_tag="VGP27_28195"
     CDS             complement(1593..2042)
                     /locus_tag="VGP27_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855213.1"
                     /translation="MSPTAAAGADDGSLNGRYVATSNGEWAMTNDQYRDETSVRSTWT
                     ITSSCADPTDCVGTMSSDRGWSADIYKKAGMWYVQHVVPAWQPCPDGTAADGVQLFTF
                     YAGDPLTAQQLPGSDTYLGDDVTTSPSGACGINKQLMVKMPFKMVAA"
     gene            2360..>2575
                     /locus_tag="VGP27_28200"
     CDS             2360..>2575
                     /locus_tag="VGP27_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE-family protein"
                     /protein_id="HEV7855214.1"
                     /translation="MNHRAVRPLAGVTTVLGIVTIVAIAIYLFQGGYAETVPVTVVSQ
                     RAGLVMNPDAKVKLLGAQVGRVASIEPV"
BASE COUNT          463 a          815 c          799 g          498 t
ORIGIN      
        1 tccgggtcat cgcgatctgc gccactccgg tcgacgatgc gacgtcagac atcttctcca
       61 cctactggat tgacgaggcc agcggaaacc acaacgaacg gttgaacgct gcacaggcgg
      121 cgttgccgga cgacatcgcg atctgggaac accagatata cctggatccg cccggcttga
      181 cgacctccga agccgccggc ttcaagcagt tacgccaatg ggcgcgcagt ttctaccccg
      241 atgcggaggc cgtcgcagcc gagccgacgc catccggtgt tcgctgaccg accacagcgc
      301 gtgagtaatg cagcgaagta cttcgagctc gtctccgtcg tcgcattcgt ggcgctgatg
      361 gtgtgggtgg tgttggacac ccgcaggcgc ggagagttgg gtgtggtcgg gttgctggcg
      421 gtcgccgggt tcagcatctt ctggcaggag ttctacgccg actggggctc ctatctgctg
      481 tggactcccg acttccacat gctgccgtgg ggtgcgacgc cgtggacaac tccagaaaag
      541 cccgcgatga atctcacctc ctatccggtc ttcatgaccg cggcgttcgt ctcgatgctg
      601 gccctacaga aatgggcgcg caggcggtgg cccggggtac atcccatggt gctcagcctc
      661 atcacggccg gcccggtcct catcggcttc aacctggtga tggaatacgt atccgtgaag
      721 acgctcggtc tgtggaccta cgtcgacacc atcggaccag tgctacacac cgatgcaggc
      781 acgatgccgc tgctgtatcc caacattccc ttcggcctct tcggagcggt caccgcgttt
      841 ctcatcggtt ggaccaatgc ggagggaagg ccgcgcttcg aagcgctcgt tgccaaacct
      901 ggcctaccgc aagggatcaa gcgcgacgtc ctgcggacaa tcgcatgggt tctaacgttc
      961 aacgtgacct actggctgtt cctgatcaca ccggccatcg tggcgcgtct gacgctgggc
     1021 gagccgtcgg ccctcgtgcc ctgacgcgaa cagttgagtg cttccacgag cggtcgttct
     1081 tgtgcttcaa cccctcacga ccgtgagagc gagatcggat acaccaatgt cccgggggcg
     1141 ccgtcgcagc ccgtgtcgaa cgacgacgtg agcgtgccgg ccatcgtggt cgcatcccat
     1201 gagtagacgt cgtgagtggt cgatgtggga ccgtaataca cgttccggca ccgcaaaccg
     1261 tatggatcgt cgacggttag ggtgtagcgg ccgtcgacga gcgcagcatc gcctgcgtaa
     1321 ttgaacgctt tcgcaacagg tctggcgatc ccgaacacat ggatgcaatc gggcggtgcc
     1381 gcaccggcga cgcatggcgt gaccgaccac acccaggtgt ggaagtcgaa cctgtcggga
     1441 atgatcagat cgaagttccc gatcgggttc gccgcgtcgg cagggggagc gatgaacgtc
     1501 gcgaccgaca ccagcacgcc gcctacggcg cccatgaggg cggccctctt catggcgcgc
     1561 tcctttcaga cgtgacccga ccgaccgagt actcaggcgg caaccatctt gaacggcatc
     1621 ttgaccatca gttgcttgtt gatcccgcag gccccgctgg gagatgtcgt gacgtcgtcg
     1681 ccgagatagg tgtcggatcc cggcaactgc tgagcggtca acgggtcgcc ggcgtagaac
     1741 gtgaacaact gcactccgtc ggcagcggtg ccgtcggggc agggctgcca ggccggcacc
     1801 acgtgctgga cgtaccacat ccctgccttc ttgtagatgt cggcagacca accgcgatcg
     1861 ctgctcatcg ttccgacgca atcggtggga tcggcacacg aggacgtgat cgtccacgta
     1921 ctgcggacgc tggtctcgtc ccggtattgg tcgttggtca tcgcccattc gccgttcgac
     1981 gtggcgacat agcggccgtt gaggctgcca tcgtcggcgc cggccgcagc ggttggcgac
     2041 acggcaacgc accctagcgt cgcggccact ccaaccgtcg tcacgccgcg cctgagcgct
     2101 cggggaggcg gaaccatcat ttccgcatgt taaagcgttc ttggttacgt cgtgcggttt
     2161 tcacgccagg tctccacgca aattcgtaag ccaatctgtc aattagtagc accttacatc
     2221 tgccaaaaaa ctaccctctc gtatccctta cgatgtagcc tgccgtgcag gagcggttcg
     2281 acgacggact acccgtccga aggttcatga ccggtgggag ggctcgtttg acggactcaa
     2341 tcggcacgac tgaggtgcga tgaaccaccg cgccgtgcgg ccccttgccg gggtcacaac
     2401 cgtcctcggg atcgtcacga tcgtcgccat cgccatctat ttattccaag gcggctatgc
     2461 ggagacggtc ccggtcacgg tggtgtcgca gcgcgcgggc ttggtgatga acccggacgc
     2521 caaggtgaaa ctgcttggtg cccaggtcgg tagggtcgcc tccatcgagc ccgta
//
LOCUS       DASYLX010001193         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_325268,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001193 DASYLX010000000
VERSION     DASYLX010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325268"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VGP27_28205"
     CDS             complement(<1..1619)
                     /locus_tag="VGP27_28205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV7855215.1"
                     /translation="MLIARLPLAALLFALAAPPSFAQGGAAVPATPQQDPRTAEPSER
                     PPAFEEQVVVSASRTEQQLVNAPASVSVVTAETIQNTPASNMGDLLRAVPGVNVSQVS
                     ARDVNITTRGATSTLSTSQLALVDGRTIYLDFFGMVMWDLVPTNQDEIKQIEVIRGPA
                     SAVWGANAMSGVVNVITKSPRELAASNGSSVTIGAGAFGRSAQGIDRDSGSLFYVNGT
                     HAQALDDRWSYKVSAGYFSQDALPRPVGTINNSFHTPYPTYANSGTSQPKFDARVDYD
                     LQNNARLTFAGGVAGTEGIIHSGIGPFDISRGSRLSYFTSRYQKGGRRVAFFSNILNG
                     DASNLLSRGANGQPLPLVFDTKTFDIEATDVHTIGTSSVISYGGNFRHNMFDISIAPG
                     GKPRNEGGVYLQDEIFLGPHFRWVVGGRVDKFSSIADAVFSPRTTLLMKPAPSQTFRV
                     SVNRAFRAPSYINNNINTTILNAVDLSAISPLLANFVFPIRAIANPDLVQETMTAYEI
                     GYTGVLRNRATVTAAVYWNNTDNGIYFTQVA"
     gene            1733..1809
                     /locus_tag="VGP27_28210"
     tRNA            1733..1809
                     /locus_tag="VGP27_28210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1767..1769,aa:Arg,seq:tcg)
     gene            1851..1927
                     /locus_tag="VGP27_28215"
     tRNA            1851..1927
                     /locus_tag="VGP27_28215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1885..1887,aa:Arg,seq:ccg)
BASE COUNT          329 a          604 c          723 g          356 t
ORIGIN      
        1 ttggcgacct gcgtgaagta gatgccgtta tcggtgttgt tccagtagac cgccgcagtg
       61 accgtggcgc ggttgcgcag cacgccggta tagccgatct cgtacgcggt catcgtttcc
      121 tggaccaggt cggggttggc gatggcgcgg atcgggaaca cgaagttggc gagcagcggc
      181 gaaatcgcgc tgaggtcgac ggcgttgagg atggtcgtgt tgatgttgtt gttgatgtag
      241 gacggtgcac ggaaggcgcg gttgaccgag acgcggaacg tctggctcgg cgccggtttc
      301 atcagcagcg tcgtccgcgg cgagaacacc gcgtcggcaa tcgaggagaa cttgtcgacg
      361 cggccgccga cgacccagcg gaaatgcggg ccgaggaaga tctcgtcctg caggtacacg
      421 ccgccttcat tgcgtggctt gccgcccggg gcgatcgaga tgtcgaacat gttgtggcgg
      481 aagttgccgc catagctgat cacgctgctg gtgccgatgg tgtggacgtc ggtggcctcg
      541 atgtcgaacg tcttggtgtc gaacaccagc ggcagcggct ggccgttggc gccgcgcgac
      601 agcagattcg aggcgtcgcc gttgaggatg ttgctgaaga aggcgacgcg cctgccgccc
      661 ttctggtagc ggctggtgaa gtaggacagt cgcgagccac gcgagatgtc gaacggaccg
      721 atgccgctgt ggatgatgcc ctcggtaccg gcgacgccgc cggcaaacgt gaggcgcgcg
      781 ttgttctgca ggtcgtaatc gacgcgggcg tcgaacttcg gctgcgacgt gccgctgttg
      841 gcgtaggtcg ggtacggcgt gtggaagctg ttgttgatcg tgccgaccgg ccgcggcagc
      901 gcgtcctggc tgaaatatcc ggccgagacc ttgtacgacc agcggtcgtc cagcgcctgc
      961 gcgtgcgtgc cgttgacgta gaagagcgag cccgaatcgc gatcgatgcc ttgcgcgctg
     1021 cggccgaagg cgccggcgcc gatcgtcacc gacgacccgt tgctcgccgc cagctcgcgc
     1081 gggctcttgg tgatgacgtt gacgacgccg ctcatggcat tggcgcccca cacggccgac
     1141 gccgggccgc ggatcacttc gatctgcttg atctcgtcct ggttggtcgg gaccaggtcc
     1201 cacatcacca tgccgaagaa atcgaggtag atcgtccggc cgtcgaccag ggcgagttgc
     1261 gaggtggaga gggtcgacgt ggcgccgcgc gtggtgatgt tgacgtcgcg ggcgctcacc
     1321 tgcgacacgt tgacgccggg gacggcgcgc aggaggtcgc ccatgttcga ggccggcgtg
     1381 ttctggatcg tctcggccgt cacgacgctc accgaggccg gggcgttgac gagctgctgc
     1441 tcggtgcggg aggcggagac gacgacctgc tcttcgaacg caggcggccg ctccgacggc
     1501 tcggccgtgc gcggatcctg ttgcggcgtt gccggtacgg cggcgccgcc ctgggcaaac
     1561 gagggtggtg cggcgagcgc gaacagcagc gctgcgaggg gcaagcgggc aatgagcaca
     1621 ggtgcactcc ttatggacca ccgggtctcc agcctgagtc cggcgctgac gcgcagcata
     1681 gtaactgcat ccgccgtgat tcgaacggcg aggtgatatg attcgtgctc gtgcgcccgt
     1741 agctcagttg gatagagcgc cggacttcga atccgtaggt cgcaggttcg agtcctgccg
     1801 ggcgcgccag acagacgaaa agaaatcccg aagccccaac tgcagtacac gcgcccgtag
     1861 ctcagctgga tagagcgttg gcctccggag ccaaaggccg caggttcgaa tcctgccggg
     1921 cgcaccacct tccgacaact ccactgctca cccgaatcgg acccagcggt gccgcacgcc
     1981 ggttgtgctc tctcgagcac ttcacgcggc gt
//
LOCUS       DASYLX010001194         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2409376,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001194 DASYLX010000000
VERSION     DASYLX010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409376"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VGP27_28220"
     CDS             <1..1590
                     /locus_tag="VGP27_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7855216.1"
                     /translation="GPSRRIIGITIISFLAMMFTPTKWTHHFGVFAGLAGSLGALAAV
                     AVTAAAMKSRRNRAMFAAAVLFVMALSFASVNGWWYVSNFGVPWSNQFPEWHFGFTTM
                     LFGLALVALLVAAWFHFYGHDISPPDGNGRRLARILQSPLAIAAWLLVVFEVLSLTLG
                     MTDLYPAWSNGRSNLGALTGKRCGLADYVMVEQDTNAGILTPASGTPADALAGSTAEG
                     FGPNGIPTNLSADEVIGGGPGTSSNFADSAQSNQDDTNQSGTEGGTTAGAGVNGSRAR
                     LPFNLDPARTPVMGSWRSGTQQPAALRSAWYRLPADWSERDRSSSILVVSAAGRFDPG
                     DIVLQWAGDDGESKGAVGFADVGAPPAWRNLRAPLGAIPADATQVRLVVKDDDLSPQH
                     WIAVTPPRIPQLKTLQNVVGSRDPVLLDWLVGMAFPCQRPFGHQNGVIEVPKWRIMPD
                     RFGAEANSPVMDNVGGGPLGISELLLRATTVPSYLKDDWFRDWGELQQFTPYYRDAQP
                     ARLELGSATRSGLWSPAPLRY"
     gene            1625..>3487
                     /locus_tag="VGP27_28225"
     CDS             1625..>3487
                     /locus_tag="VGP27_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEV7855217.1"
                     /translation="MPRDEPERTRRSATARLVAVVAGIAGVVLCALVPLLPVKQTTAS
                     ISWPQAPGADGMVADVTAPLVAGAPQALDISVPCPAIATLPAGGGLVLSTVPSVATDA
                     TRSGLFVRATSDVVFVAFRDSVAAVAQRTAIDAGNCSVLHIWANVSGVGADFVGIPGA
                     TGALAAEKKPQIAGLFTDMKVAPQPGLSARIDIDTRFITTPTPLKATVMVLGVIAVAA
                     SIIALAVLDRTSGRRVPRAWRRFVRAGPITWITDIGVVATLLLWHVIGATSSDDGYNL
                     TIARVADDAGYTANYYRFFGATEAPFDWYQSVLAHMASISTAGVWMRLPATAAAIATW
                     MIISRCVLPRLGRRVAVNSVAVLTAGAVFLAAWLPFNNGLRPEPIIAFGTIATWVLVE
                     NAIGTRRLWPAAAAIVIAMFSVTTAPQGLIALAPILVAARAIARNIRARRAVDGLLAQ
                     IAPLAASLSLIFVIVFRDQTLATVAESARIKSVVGPTIAWYQEFLRYYFLTVEDSADS
                     SLTRRFAVLVMMLCLFGMITVLLRRGSLPGVASGPVWRLIGSTAIGLLLLTFTPTKWA
                     VQFGAFAGLAGALGAVTAFVFARVGLHTRRNLALYVTALLFILAWATSGINAWFY"
BASE COUNT          504 a         1245 c         1136 g          602 t
ORIGIN      
        1 gggccgagcc gacgcattat cggcatcacc atcatctcgt tcctcgccat gatgttcacg
       61 ccgaccaagt ggacgcacca cttcggggtg ttcgccgggc tcgcgggatc gctgggcgca
      121 ctggctgcgg tcgcggtcac cgcagcggcg atgaagtcgc ggcgcaaccg ggcgatgttc
      181 gccgccgcgg tgctgttcgt catggcgttg tcgttcgcca gcgtcaacgg ctggtggtat
      241 gtctccaact tcggcgtgcc ctggtcgaac cagttcccgg agtggcactt cggcttcacc
      301 acgatgctgt tcggccttgc tctcgtcgcc ctgctggtcg ccgcctggtt ccatttctac
      361 ggccacgaca tctcgccacc tgacggcaac ggacgccgat tggcccgaat tctgcagtca
      421 ccgttggcga ttgcggcatg gctgctcgtc gtgttcgagg tactgtcgct gacactcggc
      481 atgaccgacc tctatcccgc ctggtccaac ggccgctcca atctgggggc gctgaccggc
      541 aagcgctgcg ggctcgctga ttacgtcatg gtcgagcagg acaccaacgc cggcattctg
      601 acacccgcga gcggcacgcc cgccgatgcc ctcgccggct cgaccgccga ggggttcggc
      661 ccgaacggaa tacccaccaa cctgtcagcc gacgaggtga tcgggggcgg ccccggcacc
      721 tcgagcaact tcgccgactc tgcgcagagc aaccaggacg acaccaatca gagcggcacc
      781 gagggcggca ccacggccgg cgccggagtc aacggctcgc gtgcgcggct gcccttcaac
      841 ctcgacccgg ctcgcacccc ggtgatggga agctggcgca gcggcaccca gcagcctgcg
      901 gcgctgcgct cggcctggta ccggctgccg gccgactgga gcgagcgtga tcgctcctcg
      961 tccattctcg tggtgtcggc cgcaggacgc ttcgaccccg gcgacatcgt cctgcagtgg
     1021 gcaggcgacg acggcgagtc caagggcgcg gtcggattcg ccgacgtcgg cgcgccaccg
     1081 gcctggcgga acctgagggc gccgctcggc gcgatccccg ccgacgccac ccaggtgcgg
     1141 ctcgtcgtca aggacgacga cctctcgccg cagcactgga tcgcggtcac cccgccgcgc
     1201 atcccgcagc tgaagacgct gcagaacgtc gtcggatcgc gggatccggt gctgttggac
     1261 tggctggtcg ggatggcttt cccgtgccaa cgtcccttcg gccaccagaa cggcgtcatc
     1321 gaggtgccca agtggcgcat catgcccgac cggttcggcg ccgaggcgaa ttcgccggtg
     1381 atggacaatg tcggcggcgg tccactcggc atcagcgagc tgctgctgcg ggcgaccacc
     1441 gtgccctctt atctgaagga cgactggttc cgcgactggg gtgagctgca gcagttcacg
     1501 ccctactacc gcgacgccca gccggcacga ctcgagctcg gttcggcgac gcgcagcggt
     1561 ctgtggagcc cggcaccgct gcgctattaa gcgaatgtag gtccttcgcc taccatcgag
     1621 cctcgtgcca cgcgacgaac ccgagcggac ccggcgctcc gccaccgccc gcctcgtcgc
     1681 cgtcgtcgca ggcatcgccg gagtggtgct gtgtgcgctg gttccgctgc tgccggtcaa
     1741 acagaccacc gcctccatct cctggcccca ggctcccgga gcggacggca tggtggccga
     1801 cgtcaccgcc ccactagtcg ccggcgcacc acaggccctc gacatctctg tcccctgccc
     1861 ggcgatcgcc accctgcccg ccggcggcgg tctcgtcctg tcgaccgtgc cgagcgttgc
     1921 gaccgacgcc acaaggagcg gtctgttcgt cagggccacc tccgacgtgg tgttcgtggc
     1981 gttccgcgac tcggtggccg ccgtggctca gcggacagca atcgacgccg gaaactgcag
     2041 cgtcctgcac atctgggcca acgtttccgg agtgggcgcc gacttcgtcg gcatccccgg
     2101 tgccaccggc gctctcgcgg cggagaagaa gccgcaaatc gcaggtctgt tcaccgacat
     2161 gaaggtggcg ccgcagccgg ggctgtccgc ccggatagac atcgacaccc ggttcatcac
     2221 cacgccgacg cccttgaaag cgacggtgat ggtgctgggc gtcattgcgg tggcggcgtc
     2281 gatcatcgcg ctggcggtcc ttgacagaac gagcggccga cgggtgccgc gcgcctggcg
     2341 tcggttcgtt cgcgcagggc ccatcacctg gatcaccgac atcggcgtgg ttgcaacgct
     2401 gctgctgtgg cacgtcatcg gtgccacttc ctccgacgac ggctacaacc tgaccatcgc
     2461 gagagtcgcc gacgacgctg gatacaccgc caactactac cgcttctttg gcgccaccga
     2521 ggcgccgttc gactggtacc agagcgtgct cgcacacatg gcatcgatca gcactgccgg
     2581 agtgtggatg cggttgcctg ccaccgcggc ggcaatcgcc acctggatga tcatcagccg
     2641 ctgcgtgctg ccccgcctcg gcaggcgggt agccgtcaac tctgtggccg tcctgacggc
     2701 cggagcggtg ttcctcgccg catggctgcc gttcaacaac gggctgcgcc ccgagccgat
     2761 catcgccttc ggcaccatcg ccacgtgggt gctggtggag aacgccatcg gaacccgaag
     2821 gctgtggccc gcagcggcgg cgatcgtcat cgccatgttc agcgtgacga ccgcaccgca
     2881 gggcctcatc gcgctggcac cgatcctggt cgcggcccga gcgattgcgc gcaacatccg
     2941 ggcgcgccga gccgtggacg gcctgctggc gcagatcgcc ccgctcgccg cgtcgctgtc
     3001 gttgatcttc gtgatcgtgt tccgggacca gacgctcgcc acggtcgccg agtccgcccg
     3061 catcaaaagc gtggtcggcc cgaccatcgc ctggtatcag gagttcctgc gctactactt
     3121 cctgaccgtc gaggacagcg ccgactcgtc gctgactcga cgattcgccg tgctggtgat
     3181 gatgctgtgc ctgttcggga tgatcacggt gctccttcgc cgcggcagcc tgccaggcgt
     3241 cgcgtcgggc cccgtgtggc ggctgatcgg ttccaccgcg atcgggctgc tgttgctgac
     3301 cttcacaccg acgaagtggg ccgtgcagtt cggcgccttc gccggcctgg caggtgcgct
     3361 cggagcggtc accgcgttcg tgttcgcgcg cgtcggcctg cacacccgcc gcaacctcgc
     3421 gctctatgtg accgcgttgt tgttcatact ggcctgggcc acgtcgggta tcaacgcatg
     3481 gttctac
//
LOCUS       DASYLX010001195         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2518287,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001195 DASYLX010000000
VERSION     DASYLX010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2518287"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGP27_28230"
     CDS             <1..546
                     /locus_tag="VGP27_28230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7855218.1"
                     /translation="DILVNNLGIFGAVPAQEITDEQWRTYFDVNVLAAVRLIRMYLPG
                     MTERGWGRAIQIASDSALVTPEEMIHYGVSKTALLAVSRGFAKDAAGTGVTVNSVIAG
                     PTHTAGVEDFVYQLVDRSLPWDEAQREFMRMHRPQSLIQRLIEPEEIANMVTYLALPL
                     ASATTGGALRVDGGYVDCIVP"
     gene            complement(588..1160)
                     /locus_tag="VGP27_28235"
     CDS             complement(588..1160)
                     /locus_tag="VGP27_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV7855219.1"
                     /translation="MRIIRNWLVATETILMLTLVGVGAAAPAVADPGMGSQDTASASA
                     DDTAEGGLPNGQRLSPFDLSSPAVNRLDPALLDAIQRATQSAADDGITIGLTSGWRSP
                     EFQQRLYDEAVVQYGSPDIASQYVASPQVSKHVIGKAVDVGPAAADDWLIRNGLAFGL
                     CQIYANEIWHFELASDYGSVCPPLRPNAAG"
     gene            complement(1242..1314)
                     /locus_tag="VGP27_28240"
     tRNA            complement(1242..1314)
                     /locus_tag="VGP27_28240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1279..1281),aa:Arg,seq:tct)
     gene            1629..>2042
                     /locus_tag="VGP27_28245"
     CDS             1629..>2042
                     /locus_tag="VGP27_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEV7855220.1"
                     /translation="MSNVPEPDAAGLPEELSPVDQLLHRGEANPRTRSGIMTLEVLDT
                     TPDWQRLRRRFEHASRKVLRLRQKVVMPTLPTVAPRWVIDPDFNLDFHVRRVRVAEPG
                     TLREVLDLAEVALQSPLDISRPLWTATLVEGLAGGR"
BASE COUNT          360 a          647 c          686 g          349 t
ORIGIN      
        1 gacatcctcg tcaacaacct cggcattttc ggcgcggtac cggcacaaga gatcaccgac
       61 gagcaatggc gaacctattt cgacgtcaat gtgcttgcgg cggtgcggct tatccggatg
      121 taccttccgg gcatgaccga gcgtggctgg ggcagagcca ttcaaatcgc cagtgactca
      181 gcgttggtga cgcccgagga gatgattcac tacggcgtgt ccaagacggc gctgctggca
      241 gtgtcgcgtg gattcgccaa ggatgcggcg ggaaccgggg tgacggtgaa ttcggtaatc
      301 gccggaccga cgcacacggc gggtgtcgag gacttcgtct accaacttgt cgacaggtcg
      361 ctgccgtggg acgaggcgca gcgcgaattc atgcgcatgc atcgtccgca gtcgttgatc
      421 cagcggctga tcgagccgga ggagatcgcg aacatggtga cgtacctggc cttgcccttg
      481 gcttccgcga cgactggggg agcgctgcgc gtcgacggcg gatacgtgga ttgcatcgtg
      541 ccctagcctt cggacgtcgg gattgccgag tgcgggtacc cgcggcttca gcccgcggca
      601 ttgggtcgca acggtgggca tacgcttccg tagtcggacg ccaactcgaa atgccagatc
      661 tcgttggcat agatctgaca cagcccgaac gccaggccgt tgcggatgag ccagtcatcg
      721 gccgctgcgg ggccgacgtc gacggccttg ccgataacgt gcttggacac ctgaggagat
      781 gcgacgtact gactggcgat gtccgggctg ccgtactgga cgacggcctc gtcgtatagg
      841 cgttgctgga actccggtga gcgccaaccg gaggtgaggc cgatggtgat gccgtcgtcg
      901 gccgcgctct gggtggcccg ctggatggcg tcgagcaatg ccggatcgag ccggttcacc
      961 gcggggctgg agaggtcgaa ggggctgagc cgctgaccgt tcggcagccc tccttcggcg
     1021 gtgtcgtccg ccgacgccga tgcggtgtcc tgagacccca tgcccggatc ggccaccgcc
     1081 ggagcggcag caccgacgcc gacgagcgtc agcatcaaga tcgtctcagt cgctacgagc
     1141 cagttccgaa tgatccgcac ccggtcatta tcggcggtgc cttgtgcgcg cgggaaagta
     1201 ggttggatcg gcccaccgga gccgacccaa catgcctcgg gcgcccccgg caggactcga
     1261 acctgcggcc aaccgcttag aaggcggttg ctctatccac tgagctacgg gggcagcgca
     1321 ctcaccctag ccgagctcac agatcacatc aggcccgcag aacagggcgc gtcggtcatc
     1381 ttccgggttg tcagccgcca gcggtgttgg acgctgccgg atcccgcgag ttgaagcctg
     1441 cgattcttcg ctgaccgtta cgaaggtgta tttggtgaca ccaagcgagt tggcccgctc
     1501 gaccctccga ctacccgctc tacatagcca ccaaaggcac gtcgccgcga gttacggcgg
     1561 actagcgtgg gtaacaatat gtgcgggccg gaaggaggag cagcaacgat ggcagggtgg
     1621 tcgcagccgt gagtaatgtg cctgaaccgg acgcggccgg actgccggaa gaactcagcc
     1681 ccgtcgatca gcttctgcat cgcggcgaag ccaacccccg cacgcgctcg gggatcatga
     1741 ccctcgaggt actcgacacc acgccggact ggcaacggct gcggaggcgc ttcgaacacg
     1801 cctcgcgcaa agttctgcgg ctgcggcaga aagtagtgat gcccaccctg cccacggtgg
     1861 cgccgcgatg ggtcatcgac cccgacttca acctcgattt tcacgtccgg cgcgtacgcg
     1921 tggccgaacc cggcaccctg cgcgaggtgc tcgacctcgc cgaggtggcc ctgcagtcgc
     1981 cgctcgacat ctcccggccg ctgtggaccg cgaccctggt cgagggcctg gcaggcggtc
     2041 gg
//
LOCUS       DASYLX010001196         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3614669,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001196 DASYLX010000000
VERSION     DASYLX010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614669"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGP27_28250"
     CDS             <1..613
                     /locus_tag="VGP27_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03854 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV7855221.1"
                     /translation="RVLPVFGLRSALPAERDLFVVPDGKRAAVFDESLRLLRIALRED
                     DVTFSGDYFTVRNAQVLPKPTPPLDIWIGGATAAAFRRIGTLGDGWLGSFLTPSEAAQ
                     ARQAIMAAADAAGRQIEPDHYGINLAVGNGDLPAPVLAAIRKRRPDLDPAELVAADWP
                     TLHRRLDDYISAGLTKFVIRPAGDVDTSDFLDAFVTQLLPLQN"
     gene            760..>2126
                     /gene="hrpA"
                     /locus_tag="VGP27_28255"
     CDS             760..>2126
                     /gene="hrpA"
                     /locus_tag="VGP27_28255"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEV7855222.1"
                     /translation="MAETSVGELRRRLDGLTIRDAARLGRRLKNLRGANPEKLQPLAE
                     QIAAGEALVATRQAALPIISYPDLPVSQRRQEIAEAIRTHQVVVVAGETGSGKTTQLP
                     KICLDIGRGIRGTIGHTQPRRLAARTVAQRIADELDTPLGETVGYSVRFADQVSDRTL
                     VKLMTDGVLLAEMQRDRRLLRYDTLILDEAHERSLNIDFLLGYLRELLPQRPDLKVIV
                     TSATIEPQRFAAHFDGAPIVEVSGRTYPVEIRYRPLEVPVASPDDDDPDDPDHEVVRT
                     ESRDQTEAIIDAIGELEKEPPGDVLVFLSGEREIRDTADALRHLKNTEVLPLYARLPT
                     AEQQRVFAPHTGRRVVLATNVAETSLTVPGIRYVVDPGTARISRYSRRTKVQRLPIEP
                     ISQASAAQRAGRSGRVAPGVCIRLYSEGDFESRPRYTDPEILRTNLAAVILRMAALQL
                     GDMAS"
BASE COUNT          353 a          749 c          681 g          343 t
ORIGIN      
        1 gcgggtactg cccgtcttcg gcctgcggtc ggcgttgccc gccgaacgtg acctgttcgt
       61 cgtgccggac ggaaagcgcg ctgccgtctt cgacgagtca ctgcgcttgt tgcgcatcgc
      121 gttgcgggag gacgatgtga ccttctccgg cgactacttc accgtccgga acgcgcaggt
      181 gctcccgaaa ccgactccgc cgcttgatat ctggatcggc ggagcgacag ctgcggcgtt
      241 tcggcgcatc gggaccctcg gcgacgggtg gctgggcagt ttcctgactc cgtcggaggc
      301 ggcccaggcc cgtcaagcga tcatggctgc cgccgatgca gcgggccgcc agatcgaacc
      361 tgaccactac ggaatcaacc tcgcggtcgg caacggtgat ctgcctgcgc cggtgctcgc
      421 cgcgattcgc aaacgtcggc ccgatctcga ccccgcagag ctcgttgcgg cagattggcc
      481 gacgctgcac cgaaggctgg acgactacat ctccgccggg ttgacgaagt tcgtcatcag
      541 gccggcaggc gacgtcgaca cctcggattt ccttgacgcc ttcgtcaccc agctgctccc
      601 actgcagaac tgagttcccg acgactactc cgagtcggca ccgcgttggt cgtcgagtac
      661 gccggattcg ccggcggtac ccgtgtttcg gcgccttcgg cgggctgctg ctgatcgggt
      721 ctcgatttgc ggcaaccgcc gaggactacc ctgacgggag tggccgaaac gtccgtcggg
      781 gagctgcgcc ggcgcctcga cggtctgacc atccgcgacg ccgcgcggct cggcaggcgc
      841 ctgaagaacc tccgcggcgc taatcccgag aagctgcagc cactcgccga acagatcgcc
      901 gcgggagaag cgctcgtcgc cacccgccaa gccgccctcc cgatcatctc gtacccggac
      961 ctgccggtca gccagcggcg acaagagatc gccgaggcga tccggacaca tcaggtcgtg
     1021 gttgtcgcgg gggagaccgg ttccggtaag acgactcagc tgcccaagat ctgcctcgac
     1081 atcggccgag gtatccgcgg cacgatcggc cacacccagc ccagacggct ggccgcgcgc
     1141 accgtggccc agcgcatcgc cgacgagctg gacacaccgc tgggggagac ggtcggctac
     1201 agcgtccggt tcgccgatca ggtcagcgac cgcacgctgg tcaagctcat gacggacggt
     1261 gttctgctcg ccgagatgca gcgtgatcgc cgcctgttgc ggtacgacac cctcatcctc
     1321 gacgaggccc acgagcgcag cctcaacatc gacttcctgc tcggctatct gcgcgagctg
     1381 ctgccacaac ggcccgacct gaaggtcatc gtcacctcgg cgaccatcga gccgcagcgc
     1441 ttcgcggcac acttcgacgg cgcccctatc gtcgaagtgt cggggcgcac ctatccggtc
     1501 gagatccgtt accgcccact ggaagttccg gtcgcgtcac ctgatgacga cgaccccgat
     1561 gatcccgacc atgaagtcgt ccgcaccgag tcgcgcgacc agaccgaggc gatcatcgac
     1621 gccatcggcg aactcgagaa ggagccgccc ggcgacgttc tggtgttctt gtcgggtgag
     1681 cgcgagatcc gcgacacggc agacgctttg cggcatctga agaacaccga ggtactgccg
     1741 ctctacgcgc gtctgccgac cgccgaacaa cagcgagtct tcgctccgca cacgggtcgg
     1801 cgagtcgtgc tcgcgaccaa tgtcgccgag acatcgctga cggtgccggg gatccggtac
     1861 gtcgtcgacc cgggcaccgc acggatttca cgctacagcc gcagaacgaa ggtgcagcgg
     1921 ctgccgatcg aacccatctc gcaggcgtcc gccgcccagc gcgcggggcg ctcgggacgc
     1981 gtggcgccgg gcgtgtgcat ccgcctgtac tccgagggcg atttcgaatc acggccgcgc
     2041 tataccgacc ccgagatcct gcgcacgaat ctcgccgcgg tgatcctgcg gatggcggcg
     2101 ctccaactcg gcgacatggc gagctt
//
LOCUS       DASYLX010001197         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_103909,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001197 DASYLX010000000
VERSION     DASYLX010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103909"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VGP27_28260"
     CDS             <1..735
                     /locus_tag="VGP27_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7855223.1"
                     /translation="VLIFVPGGAWVHGSRTFQGSALMSHLVESGWICLAIDYRVSPRN
                     RWPRHIMDVKAAISWARANADRFGGDRDFVAIAGCSAGGHLASLAALTPGDRRFEGEL
                     DPNSETSVDAAVSIYGRYDWHDRSTPERDGFVDFLESIVVQRRYDRHEEIYRDASPIA
                     RVTDSAPPFFVIHGGSDFVIPVAQAQTFVDELRATSRNAVGYLELPGAHHGFDLVDGK
                     CTGSACTAIGLFLNEIHRRHTLAAVS"
     gene            776..2197
                     /locus_tag="VGP27_28265"
     CDS             776..2197
                     /locus_tag="VGP27_28265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEV7855224.1"
                     /translation="MKRLSGYDAMLLYSETPAVHTHTMKIAVVDGGPTGLTFETFREL
                     VRQRLPVMEPLHYRLIDIPWKLHHPMWQENCDVDLDYHLRRSQAKGDGSRRSLDEVIG
                     EIASTPLARDRPLWEFHFVEGLAGNRVAVVGKVHHSLADGVASANLMAKAMAYTTGEP
                     ETIPAADPMPTSRRLISAAARDLVGQMAQLPALFGYTAAGLTKVVRRSLQRTRHPSIA
                     SGLNAPDTFMNHIVSARRMYASATLALADVKEVSKALGITINTLVLAVAAGALRELLL
                     RYDGHADEPIVASVPSSLDLSPDRIAGNELGGMVVTLPVQVDDPLERTRLTDLAAGVA
                     KENYRLLGPEFSARWMEFLPPALAPALFAWSARRDAKLRVFNVPVSNVPGPRERGRFG
                     DITLTELYSVGPLTAGCGLNVTVWSYVDQFNISVLADDETVRDPHEVTDAMVRSFGEI
                     RRAAGFSSASTAVPTAMELARPA"
     gene            complement(2194..2523)
                     /locus_tag="VGP27_28270"
     CDS             complement(2194..2523)
                     /locus_tag="VGP27_28270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7855225.1"
                     /translation="MIFIVVKFKPKAEYVDSFPDFVAEFTEATRNEPGNMFFDWSRSL
                     DDPSEYVLVEGFRDGDAGKKHVTSEHFKKFVAEAPKVLAATPLIISHTIDADGWSEMG
                     EMKVGQR"
     gene            complement(2543..>2723)
                     /locus_tag="VGP27_28275"
     CDS             complement(2543..>2723)
                     /locus_tag="VGP27_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV7855226.1"
                     /translation="EINFIGNLVGSYNDLCDLMALAASGAVNLHTQKYALDDFQSAIT
                     DLAKGNVRGRAILTP"
BASE COUNT          468 a          903 c          850 g          502 t
ORIGIN      
        1 gtgctgatct tcgttcccgg cggcgcctgg gtgcacggca gccgcacctt ccaggggtcg
       61 gcgctgatgt cgcatctcgt cgagtccggt tggatttgtc tcgcgatcga ctaccgggtg
      121 tccccgcgga accgctggcc ccgccacatc atggacgtga aggccgcgat ctcgtgggcc
      181 cgcgccaacg ccgaccggtt cggcggcgat cgggacttcg tcgccatcgc aggatgctca
      241 gcaggcggcc atctcgcgtc gttggccgcg ctcaccccgg gcgaccgccg attcgaaggt
      301 gaactggatc cgaactcgga gacctccgtg gacgccgccg tctccatcta cggacgctac
      361 gactggcatg accggtccac gccggagcgg gacggattcg tcgacttcct ggaaagcatt
      421 gtggttcagc gtcggtatga ccgccatgag gaaatctacc gcgacgcctc cccgatcgcc
      481 cgcgtcaccg acagtgcacc accgtttttc gtcatccacg gtggctcgga tttcgtcatc
      541 cccgtcgcgc aggcacagac gttcgtcgat gaacttcgcg ccacctcccg aaacgccgtc
      601 ggctacctcg agctgccagg cgctcaccac ggcttcgacc tcgtcgacgg aaagtgcacc
      661 ggctcggcat gtaccgcgat cgggttgttc ctcaacgaga ttcaccgacg ccacacactc
      721 gccgccgtct cctgagctga cgtcagcgcg cgtgtgatgg gagaagggac agccggtgaa
      781 gcgcctcagt ggttacgacg cgatgttgct ctacagcgaa accccggccg tgcacacgca
      841 caccatgaag atcgcggtcg tcgacggcgg tccgacgggt ctgaccttcg aaacctttcg
      901 tgaacttgtg cgccaacgtc ttccggtgat ggagccgctg cactaccgac tcatcgacat
      961 cccatggaaa cttcaccacc ccatgtggca ggagaactgc gatgtcgatc tcgactacca
     1021 cctccggcgg tcgcaggcaa aaggagacgg cagcaggcgc agcctcgacg aggtgatcgg
     1081 cgagatcgcg agcactccgc ttgcccgcga ccggccattg tgggagtttc acttcgtcga
     1141 ggggctggcg gggaaccgcg tcgccgtggt gggaaaggtg caccactcgc tcgccgacgg
     1201 tgtcgcctcg gcgaatctca tggccaaggc catggcctac acgaccggtg aaccggagac
     1261 tatccccgcg gccgatccga tgccgacttc gcgccggctg atcagcgcag cggcgcgcga
     1321 tcttgtcggc cagatggctc agcttcccgc gttgttcggc tataccgcag cgggtctgac
     1381 caaggtggtt cgaagatcgt tgcagcgcac ccgacatccg tcgatcgcca gcggactcaa
     1441 cgcacccgac accttcatga accacatcgt ctcggctcgg cggatgtatg ccagcgcgac
     1501 attggcgctg gccgatgtca aggaggtcag caaggcgctc ggaatcacca tcaacaccct
     1561 tgtcctcgcg gtggcagccg gtgcgcttcg agaactgctg ctccgctacg acggccacgc
     1621 cgacgagccg atcgtcgcct cggtgccatc gagcctcgac ctgtccccgg accggatcgc
     1681 gggcaacgaa ctgggcggca tggtggtgac gctgccggtg caagtggacg atccgctgga
     1741 gcggaccagg ttgacggatc tggccgccgg cgtggccaag gagaactatc ggctgctcgg
     1801 accggagttc tcggcgcgat ggatggagtt cctgccgccg gcgttggcac ccgcgctgtt
     1861 cgcgtggtca gcgcggcgtg acgccaagct gagggtgttc aacgtgccgg tctccaacgt
     1921 gccaggaccg cgcgaacgcg gccggttcgg agatatcact ctcaccgagt tgtactcggt
     1981 cggaccgctc accgccggct gcgggctgaa cgtcaccgtg tggagttacg tcgaccagtt
     2041 caacatctcc gtgctcgccg acgacgaaac cgtgcgcgac cctcacgagg tgaccgacgc
     2101 tatggtgcgg tcgttcggcg aaatccgccg tgccgcaggt ttttcgtccg cgagcaccgc
     2161 cgtgcccacg gccatggagc tcgcgaggcc tgcctaacgc tggcccacct tcatctcgcc
     2221 catctccgac cacccgtccg cgtcgatcgt gtggctgatg atcagcggag tggcggccag
     2281 gaccttcggt gcctcggcga cgaacttctt gaagtgctcg ctggtgacgt gcttcttgcc
     2341 ggcatcgccg tcgcggaatc cctccaccag aacatattcc gacgggtcgt cgagactgcg
     2401 cgaccagtcg aagaacatgt tgcccggctc gttgcgggtc gcctcggtga actcggcgac
     2461 gaagtcgggg aagctatcga cgtactccgc tttcggcttg aacttgacca ctatgaaaat
     2521 cactgcagat cattccttct ctctaaggcg tgagtatggc ccggccacgg acattaccct
     2581 tggcgagatc ggttatcgcc gactgaaagt cgtcgagagc gtacttctgg gtatgcaggt
     2641 tgaccgcgcc gctcgctgcg agcgccatca ggtcgcacag gtcgttgtag gacccgacca
     2701 ggttaccgat gaagttgatc tcg
//
LOCUS       DASYLX010001198         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_873327,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001198 DASYLX010000000
VERSION     DASYLX010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873327"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGP27_28280"
     CDS             <1..544
                     /locus_tag="VGP27_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7855227.1"
                     /translation="PDQLEPLEALGRHLGAAFQITDDLLNLRADPEDYGKEIGGDLWE
                     GKRTLMLLHATRNAPSDDQERAVQILAKRRPSADDELKLDDLLDRLFDRGDLSQAGRN
                     EIAAQVQGQRNLDSKSLDDIRWLYGLMQRVGSFKHAKQVAAGHTRDAAAILAGLDWLP
                     ASKHRDALASLVEYVHGRTR"
     gene            541..999
                     /locus_tag="VGP27_28285"
     CDS             541..999
                     /locus_tag="VGP27_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855228.1"
                     /translation="MKPEQLMGMLAELERIRELTLDLVERNAPRLLPPPPDDSAGPER
                     SMFDLLVGARRAVLGNPTGARHLHDMLVAEGRRYADTPEGAELRDALINSEAVENLRR
                     VWETISLNVLDGPAAPNTAPTAWADLVADAVIGHGLDDSILGRLRPEGFA"
     gene            996..1277
                     /locus_tag="VGP27_28290"
     CDS             996..1277
                     /locus_tag="VGP27_28290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855229.1"
                     /translation="MTLIDPRETLAEQSTFIGYLMGLSSLGQVVRALVAGDDSKTDRP
                     GDVDDFVHLLLGFASLGTSIEQLAETSRDENPCEADDSAIDAAYTRWLR"
     gene            1274..2293
                     /locus_tag="VGP27_28295"
     CDS             1274..2293
                     /locus_tag="VGP27_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855230.1"
                     /translation="MTAVLAGSDFVRTPILAAAQPEGFKEWHHFVIHGADRRLLINFN
                     LTSEPSRTGHRLAPRVIVIDHDKQWTGAIERFDDSELDISADLGTITIGGNRMVVQPD
                     GYQVVIDLPKQEIHGELQFTSVSRPFVVNNQPLGEGRMCWLFVPKLRADGWLRIGGQE
                     HRIDSQLAYHDHNWGRFWWGDDFGWTWGTMLSQTPGNPWSLVFLQMTDRRRLRCLSQA
                     LYVWHHDEPAAIFRHAALRTRHHGLLGRVADCTLPAPMRLLLDGEVSDVPQRVEIDAT
                     RAGDSVYAEFRPHSYARLAQPSELSLDRSTVLCETSGTAKVSGSINGEDFGFAGSGVF
                     EFLHG"
     gene            2286..2687
                     /locus_tag="VGP27_28300"
     CDS             2286..2687
                     /locus_tag="VGP27_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP-2 sterol transfer family protein"
                     /protein_id="HEV7855231.1"
                     /translation="MAERVSSLLRQSVEHLADEVPDSYRLVLDRLGPMVVELEIDGEL
                     LSLRGGQRLHVSDGAADTAGTRIATSRAAILDVLDARIGLAEAVEAGAVAVLGSLDDV
                     QRAHDTMLAYVHAAIRAPSQPGLLAALRAEQ"
     gene            2687..>3268
                     /locus_tag="VGP27_28305"
     CDS             2687..>3268
                     /locus_tag="VGP27_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7855232.1"
                     /translation="MTEAIATPGSCNRRRTVAVLGGGIAGLTAAQELAERGFDVTVYE
                     FRSDERHGLGNTPAGTYPPVKLGGLAASQYSTVGPYGGSLAQLRPFPGRRGHPRNPAT
                     AVAGEHGFRFFPAYYIHIWDLFQRIPVFQRTETGYGQVQWQATSRTIMDNLRRVVTQG
                     TTLEGKPSLVFPREAPRSPAEFLTTASQLTQLGF"
BASE COUNT          604 a         1089 c         1009 g          566 t
ORIGIN      
        1 acctgatcag ctcgaaccgc tcgaggcgct cggcaggcat ctcggcgccg ccttccagat
       61 caccgacgac cttctgaacc tgcgtgcgga cccagaggat tacggcaagg agatcggcgg
      121 tgacctctgg gagggcaagc gcaccctgat gttgttgcac gccacgagaa atgctccctc
      181 cgatgaccaa gaacgtgccg tgcagatcct ggccaagcgc cgaccgagcg ccgacgacga
      241 gctaaaactc gacgaccttc tcgatcggct cttcgaccgc ggagatctgt cccaggctgg
      301 acggaacgaa attgccgctc aggtccaggg acaacgcaac ttggactcga agagcctcga
      361 cgacatccgg tggctatatg ggctgatgca gcgggtcggc tcgttcaagc acgcaaagca
      421 ggtggctgcc ggacacactc gggatgcagc cgccatactg gccggcctcg actggttgcc
      481 ggcgagcaag catcgcgacg cgctggcctc tctcgtcgaa tacgtacatg ggcgcacacg
      541 atgaagcccg aacagttgat gggcatgctc gcggagctgg aacgcatccg cgagctcacg
      601 ctcgatctcg tcgagcgcaa cgcccctcgg ctgcttccac caccgcctga tgattccgcg
      661 ggaccggaac gatcgatgtt cgacctactc gtcggtgctc ggcgcgcggt gctcggaaat
      721 ccaaccgggg cacggcacct gcacgacatg ctcgtcgccg aggggcgccg ctacgcggac
      781 acacccgaag gcgcggagct acgcgatgcg ctgatcaact cggaggccgt cgagaatctt
      841 cgtcgcgtat gggagacaat cagcctcaac gtccttgacg gccctgccgc gcccaacacg
      901 gcaccgaccg catgggccga tctggtggcc gacgcagtca tcgggcacgg gcttgacgat
      961 tccatcctgg gccgcctgcg tccggagggc ttcgcatgac gctcatcgat ccgcgcgaga
     1021 cactcgccga acagagcacc ttcatcgggt atctgatggg gctgtccagt cttggtcagg
     1081 tggtacgggc gcttgtcgcc ggtgatgaca gcaaaaccga taggccgggc gacgtcgacg
     1141 acttcgtcca cctgctactc ggattcgcaa gcctcggtac ctcgatcgag cagctggccg
     1201 aaaccagccg ggatgaaaac ccttgcgagg cagatgattc ggccattgac gcggcgtaca
     1261 cgcggtggtt gcgatgaccg ccgtgctcgc aggcagcgac ttcgtccgca ccccgatcct
     1321 cgccgccgcc cagccggagg gattcaagga gtggcaccac ttcgtgatcc acggcgcgga
     1381 tcgacggcta ttgatcaact tcaacctgac gagtgaaccg tcacgcaccg gccaccggct
     1441 cgcaccacgt gtcatcgtca tcgaccacga caagcagtgg acgggagcga tcgagcggtt
     1501 cgacgattcc gagctggaca tctccgcaga tctcggcaca atcaccatcg ggggaaaccg
     1561 aatggtcgtc cagcccgatg gataccaggt ggtcatcgat cttccgaagc aggaaatcca
     1621 cggcgagctt caattcacct ccgtcagtcg acctttcgtc gtcaacaatc agccgctcgg
     1681 tgaaggccgg atgtgttggt tgttcgtgcc taagctgcgc gccgacgggt ggcttcgcat
     1741 cggtggccaa gagcatcgca tcgacagcca gttggcctat cacgatcaca actggggtcg
     1801 cttctggtgg ggcgacgact tcgggtggac ctggggcaca atgctttcgc aaacaccggg
     1861 aaacccttgg tcgctcgtct tcttgcagat gaccgacaga cgcaggttgc gctgcctgtc
     1921 gcaggcgctc tacgtgtggc atcacgatga gcccgccgcg atcttccggc acgccgcgct
     1981 gcgaacgcgc catcacggtc tactcggtcg agtcgcggat tgcacgctgc ccgcccctat
     2041 gcgactcctg ctcgacggcg aggtctcgga tgtccctcaa cgagtcgaga tcgacgcgac
     2101 acgagcaggc gactcggtgt acgccgagtt ccgcccgcac tcctacgccc gactggctca
     2161 accgagcgag ctcagcttgg atcggtcgac cgtgctctgc gagaccagcg gtacggccaa
     2221 ggtgagtggt tcgatcaacg gcgaggattt cggcttcgcc ggctcaggtg tattcgagtt
     2281 tctccatggc tgaacgagtt tcgtcattgc ttcggcagtc ggttgagcac ctcgccgatg
     2341 aggtgcccga cagctaccgc ctggtgctcg atcgactcgg gccgatggtg gtcgagctgg
     2401 aaatagacgg tgagctctta tcgttgcgcg gcggccagcg actgcacgtg tccgacggcg
     2461 cggcggacac tgcaggtact cggatagcca cctcgcgggc cgcgatcctc gacgtgctcg
     2521 acgccagaat cgggctcgcc gaagccgtgg aagccggcgc cgtcgccgtc ctcggttcac
     2581 tcgacgacgt tcagcgcgca cacgacacca tgctcgccta cgtgcacgct gcgatccggg
     2641 caccgtcgca gcccggccta ctggcggcac tacgggcgga acagtgatga ccgaagcgat
     2701 cgccaccccc ggctcgtgca atcggcgccg cacggtcgca gtactcggcg gcggcatcgc
     2761 cgggctcacc gctgcccagg aactggccga acgcggcttc gacgtcaccg tgtatgaatt
     2821 ccgatcagac gaacgccacg gcttgggcaa cacgcccgcg ggcacatacc cgccggtgaa
     2881 gctcggtggt ctggccgctt cgcagtattc aacggtgggc ccttacggcg gaagcctggc
     2941 ccagttgcgc cccttcccgg ggcgccgcgg ccatccgcgc aatcctgcga ccgcggtcgc
     3001 aggcgagcac ggcttccgct tctttcccgc ctactacatc cacatttggg acttgtttca
     3061 acggattccg gtcttccagc gcacagaaac tggttacggc caggtccaat ggcaggcgac
     3121 ctcgcgaacg atcatggaca acctcaggcg ggtggtcacc caaggcacca cgttggaagg
     3181 caagccgtcg ctggtttttc cccgcgaggc gccacgcagc ccggccgaat ttctcaccac
     3241 cgcaagccag ctcacccagc tgggcttc
//
LOCUS       DASYLX010001199         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2737871,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001199 DASYLX010000000
VERSION     DASYLX010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2737871"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2203)
                     /locus_tag="VGP27_28310"
     CDS             complement(<1..>2203)
                     /locus_tag="VGP27_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEV7855233.1"
                     /translation="RHRRAYPENPTERVHRRLDVGGEYAFRREGELHLFTPEVVFLLQ
                     HSTRTKRHDIFAQYSDEVDRLSREGGALRGLFELKTGMRPPVPIDEVESVDSIVTRFN
                     TGAMSYGSISAEAHETMAIAMNNLGGRSNSGEGGEDVDRLYDPRRRSAVKQVASGRFG
                     VTSDYLVNASDIQIKMAQGAKPGEGGQLPGYKVYPNIAKTRHSTPGVGLISPPPHHDI
                     YSIEDLAQLIHDLKNANSDARIHVKLVSSVGVGTVAAGVSKAHADVVLISGYDGGTGA
                     APLTSLKHAGAPWEIGLADAQQTLILNGLRDRITVQCDGGMRTARDVVVAMLLGAEEF
                     GFATAPLVVSGCIMMRVCHLDTCPVGVATQNPELRSRFNGKPEFVENYFRFIAEDIRR
                     YLAELGFRSIDEAVGHAEMLDTAEGIAHWKAKGLDLTPIFATVSNAHDPDQTVRHRVR
                     DQYHALDQALDQTLIQLAEGALEDAHPVRLELPVRNVNRTVGTMLGAEVTRRYGAQGL
                     PHDTIHITLSGSAGQSIGAFLPSGITLELIGDANDYVGKGLSGGRVVVKPPEDVLFLP
                     EDHVIAGNTLLYGATSGEVFLRGRVGERFCARNSGALAVTEGVGDHACEYMTGGRVVV
                     LGATGRNLAAGMSGGIAFVLGLDPAMVNTEMVELQRPDPEDLAWLHDVVTRHARLTGS
                     TVAASLLSDWPRRSAQFTKVMPTDYQRVLTATRMAKAEGRDVDTAIMEASRG"
BASE COUNT          352 a          714 c          744 g          393 t
ORIGIN      
        1 agccacggct ggcctccatg atcgcggtgt cgacgtcgcg gccctcggcc ttggccatcc
       61 gggttgcggt cagcacacgc tggtagtcgg tcggcatgac cttggtgaac tgcgcactgc
      121 gccttggcca gtcggacagc agggatgcgg cgaccgtgct gccggtgagc cgggcatggc
      181 gggtgaccac gtcgtgcagc caggcgaggt cctcggggtc gggacgttgc aactcgacca
      241 tctcggtgtt gaccatcgcc gggtccagtc cgaggacgaa tgcgatgccg cccgacatgc
      301 ccgcggccaa gtttcgtccg gttgccccga gtaccacgac acggccaccg gtcatgtact
      361 cgcaggcatg gtcaccgacg ccctcggtga ccgccagcgc accggagttg cgggcgcaga
      421 agcgctcacc cacccggccg cgcaggaaca cctcgccgga tgtggctccg taaagcagtg
      481 tgttccccgc gatcacatga tcctcgggca ggaacagcac gtcttcgggt ggtttgacca
      541 cgacacgtcc gcccgaaagg cccttgccga catagtcgtt cgcgtcaccg atcagttcaa
      601 gggtgattcc cgagggtaga aacgccccga tcgactgacc ggccgagccg ctgagcgtga
      661 tgtggatggt gtcgtgcggc aagccttgtg cgccgtatcg gcgggtgacc tcggcgccga
      721 gcatggtgcc cacggtgcgg ttgacgttac gcaccggcag ctccagccgc acggggtggg
      781 cgtcctcgag tgcgccctcg gcgagctgga tcagcgtctg gtccaaggcc tggtccagcg
      841 cgtggtactg gtcgcggaca cggtgacgca cggtttggtc gggatcatgg gcgttgctta
      901 ccgtggcgaa gatcggcgtc agatcgagac cctttgcctt ccaatgggcg atgccttctg
      961 cggtgtcgag catttcggcg tggccgacgg cctcgtcaat gctgcggaag ccgagctcgg
     1021 ccaggtagcg ccggatgtct tcggcgatga accggaagta gttctccacg aactcaggct
     1081 tgccgttgaa ccgcgaccgt agttcggggt tctgagtcgc gacgccgacg gggcaggtgt
     1141 cgagatggca gaccctcatc atgatgcagc cggacaccac caacggcgcg gtggcgaagc
     1201 cgaactcctc ggcgccgagc agcatcgcga cgaccacatc gcgggcggtg cgcataccgc
     1261 cgtcgcattg cacggtgatg cggtcgcgta acccgttgag tatcagcgtc tgctgagcgt
     1321 cggccaggcc gatctcccac ggcgcgcccg cgtgcttgag cgacgtcagc ggcgccgcgc
     1381 cggtgccacc gtcatacccg gagatcagca cgacgtcggc gtgcgccttg gacaccccgg
     1441 ccgcaacggt tccgacgccg accgagctga ccagcttgac gtggatgcgc gcatcggagt
     1501 tggcgttctt caagtcgtga atgagctgcg ccaggtcctc gatcgaataa atgtcgtggt
     1561 gcggcggagg cgagatcaac ccgacgcccg gcgtcgaatg gcgggtcttc gcgatgttcg
     1621 ggtagacctt gtagcccggc agctgaccgc cctcgcccgg cttggcaccc tgcgccatct
     1681 tgatctggat gtcggatgcg ttcaccagat agtcgctggt gacgccgaag cggcccgatg
     1741 ccacctgttt gacggcactg cgcctgcgcg ggtcgtatag ccggtcgacg tcttcgccgc
     1801 cctcgccgga gttggaccgc ccgcccaagt tgttcatcgc gatcgccatg gtttcgtgcg
     1861 cctcggccga gatggagccg taactcatcg cgccggtgtt gaaccgggtg acgatcgagt
     1921 cgacggattc gacctcgtct atcggcaccg gaggccgcat tcccgtcttc agctcgaaca
     1981 ggccacgcag ggctccgccc tcacgagaca gccggtcgac ctcatccgag tactgcgcaa
     2041 agatgtcgtg acgcttggtg cgggtcgagt gttgcagcag gaagacgact tcgggggtga
     2101 acaggtgcag ctcgccttcc cgccggaagg cgtactcgcc gcccacgtcg agacggcggt
     2161 gtacccgctc cgtcgggttc tccggatacg cgcgacgatg tcg
//
LOCUS       DASYLX010001200         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1310173,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001200 DASYLX010000000
VERSION     DASYLX010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310173"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGP27_28315"
     CDS             complement(<1..597)
                     /locus_tag="VGP27_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7855234.1"
                     /translation="MTTSILRTADAWWVQTSTGAAKIATTATTTRELLADRAAIDAAA
                     HSRDIVPVDGLTLVSPVTAPCRVVAQMTNYASHVTDAGMDPKSIPLTFFRKASGSISG
                     PFDDIVRPEHVRFMDYEVEIGLVIARDIPVGTTIIAENLADYIAGLVVTNDVSARDVQ
                     LPQTQFYEAKSYPTFTPVGPAVVLLDADELKRFGDLRLQ"
     gene            complement(594..1724)
                     /locus_tag="VGP27_28320"
     CDS             complement(594..1724)
                     /locus_tag="VGP27_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016895408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7855235.1"
                     /translation="MSGTVGAHNELHSERGALPGEHPGRARNPVIKVADIAWLEFEKP
                     DLDRAEAFARAFGFNTVLRTPDELRLRGTDAKAPCVLLRRGARSRFVGTAFTAVDELD
                     VRKLADATGAPTKPLPDSVGGVAVDLVDPSGLPVRVVAGMHQVPELTPQQPHVFNFGR
                     EVNRANGTQRPPRKPAAVQRLGHVVLQTTKYTEALNFYLDKLGMIVSDFLFYPDQRAR
                     GPVMSFIRCDRGSIPADHHTVALALGPSSRYIHSAYQVCDLDALAAGGEYLSGQGYFR
                     SWGIGRHIQGSQLFDYWRDPDGFLVEHFTDGDMFDNTLEPGWAPFTATGLAQWGPPVT
                     KDFLGINPSALPHEALSVVNALRTDNEFTLTRLRGLLKVANS"
     gene            1832..>2151
                     /locus_tag="VGP27_28325"
     CDS             1832..>2151
                     /locus_tag="VGP27_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV7855236.1"
                     /translation="MTDPSEAPVNRLTRRKQRTRAALIKAAQAFIAAGKMNVPVLEIT
                     QAADVGMGSFYNHFQSKEQLFDAAVAEVLDAHGALLDHLTASLDDPAETFACSFRLTG
                     RLFR"
BASE COUNT          357 a          654 c          756 g          384 t
ORIGIN      
        1 ttgcagccga agatccccga accgcttgag ctcgtcggcg tccagcagca ccacggcggg
       61 gccgacgggg gtgaacgtgg ggtaggactt ggcttcgtag aactgcgtct ggggcagctg
      121 cacgtctcgg gcggacacgt cgttggtgac gaccaggccc gcgatgtagt cggcgaggtt
      181 ctccgcgatg atggtggtgc cgaccggaat gtcgcgtgcg atgacgaggc cgatctccac
      241 ctcgtaatcc atgaagcgga catgctcggg cctgacgatg tcgtcgaacg gcccgctgat
      301 cgacccggac gccttgcgga agaaggtcaa tgggatcgac ttcgggtcca ttccggcgtc
      361 ggtgacgtgc gaggcgtagt tcgtcatctg tgcgacgacc cggcacggcg cggtgaccgg
      421 cgataccagc gtcaggccgt cgacgggcac gatgtcgcgg ctgtgcgctg cggcgtcgat
      481 ggccgcgcgg tcggcgagca gttcccgtgt ggttgtcgct gtcgtcgcta tcttggctgc
      541 gccagttgac gtttggaccc accatgcgtc ggcggtacgg aggatggaag tggtcatgag
      601 ttggctacct tcaggagtcc gcgcaggcgg gtaagggtga attcgttgtc ggtacggagt
      661 gcgttgacga ccgagagcgc ttcgtggggg agcgcgctcg ggttgatgcc gaggaagtcc
      721 ttggtgaccg gcggccccca ctgcgcgaga cccgtggcgg tgaacggtgc ccagccgggc
      781 tccagtgtgt tgtcgaacat gtcgccatcg gtgaagtgct cgacgaggaa gccgtccgga
      841 tcgcgccagt agtcgaagag ctggctgccc tggatatgcc tgccgatgcc ccaggatctg
      901 aaataccctt gcccgctgag gtattcgccg cctgcggcaa gggcgtcgag gtcacacacc
      961 tgatacgcgg agtggatgta gcggctgctc gggcccaagg ccagtgcgac tgtgtggtgg
     1021 tcggccggga tggagccgcg gtcgcagcgg atgaagctca tcaccggccc cctggcgcgc
     1081 tggtccgggt agaagaggaa gtcgctgacg atcatgccga gtttgtcgag gtagaagttc
     1141 agcgcctcgg tgtacttggt ggtctgcaat acgacatgac cgagtcgctg cacggcggcg
     1201 ggcttgcggg gcggccgctg cgtgccattg gcgcgattga cttcacggcc gaagttgaag
     1261 acgtgaggct gctgaggtgt cagctccggc acctgatgca ttcctgcaac cacccggacg
     1321 ggaagaccgc tcgggtcgac gaggtccacc gcgacgccgc cgacggagtc cggcagtggc
     1381 ttcgtgggcg ctccggtggc atcggcgagt ttgcgcacgt ccagttcgtc gacggcggtg
     1441 aatgcggtcc cgacgaatcg ggaccgagcc ccccggcgga gcagcacgca cggcgctttc
     1501 gcatcggtgc cgcgcagccg cagctcgtcg ggcgtgcgca gaacggtgtt gaagccgaat
     1561 gcgcgggcga acgcctcggc gcggtccaga tctggcttct cgaactccag ccaggcgatg
     1621 tcggcgactt tgatgacggg attgcgcgcc cggccgggat gctcgcccgg caacgcaccc
     1681 cgctcgctgt gcagctcgtt gtgggcgccc acggtgccgc tcatcagaaa cctcctcatg
     1741 aagttgacga aatcgtcaca tactcggtag ggatccgtca acagcttctg acgaaatcct
     1801 caaaactgac atattctgct accgtgagga cgtgaccgat ccatcggagg cgcctgtcaa
     1861 ccgcctgaca cggcgcaaac agcgcacccg cgccgccctg atcaaggctg cgcaggcgtt
     1921 catcgccgcg ggcaagatga acgtgccggt gctggagatc acccaggcgg ccgacgtggg
     1981 aatggggtcg ttctacaacc acttccagag caaggagcag ttgttcgatg ccgccgtcgc
     2041 cgaggtgctc gacgcgcacg gcgcgctgct ggaccatctg accgcatcgc tcgacgaccc
     2101 ggccgagacc tttgcctgca gcttccgcct caccggccgg ttgttccgca g
//
LOCUS       DASYLX010001201         7034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1420586,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001201 DASYLX010000000
VERSION     DASYLX010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420586"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VGP27_28330"
     CDS             <1..1419
                     /locus_tag="VGP27_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7855237.1"
                     /translation="APAVADDNISLNNGEFMEAVRRASAALHRHGVADGDVVAAMLPN
                     TTALVVTLFAAWRLGAAVTPINPALQPVEVGYQLADADAKVLIVDTVPNFDIGDRAVV
                     TASELTSTPATGPLRPAHLADDALALLIYTSGTTGRPKGVMLDHCNLIAMCRGVIDAV
                     ALTSADHSLLILPLFHVNGIVISTLAPLLAGGRTTIAGRFSPKTFFDRIEQCGATYFS
                     AVPTIYTILSGLPAAVKPDTSSVRFAICGAAPASIELLEQFESRYGIPIVEGYGLSEG
                     TCASTLNPLVGQRKAGTVGLPLPGQTIRMVDSNGAPVSSGEAGEVVIKGPNVMRGYLN
                     RPEETEKTIVDGWLHTGDVGRFDEDGYLVLVDRAKDMIIRGGENIYPREIETVVHQLP
                     EIAEAAVVARSNPVYGEEPVLFVSLHPDRQLGVDVIRAHLAESLSKYKRPVDITILDD
                     LPKNAVGKIAKPILRQRLTPTH"
     gene            1454..3277
                     /locus_tag="VGP27_28335"
     CDS             1454..3277
                     /locus_tag="VGP27_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEV7855238.1"
                     /translation="MGFTKPGLPNVDPDTFLQKPLMERMRILALNWGENGFGSPKMIH
                     TVYIAKLVLLYAFGGVVVATLTSGLPAFWHVSEWWNQPIVYQKVILWTVLLEVIGIAG
                     SWGPLAGKVKPMTGGILFWARPGTIRLRPWKWMPFTTGDRRTWFDVALYVALLISVVV
                     PLLSPGVHSDSLSRVMPENTSGLVSPALLITPIALLVAMGLRDKTVFLGARGEQYLPA
                     LVLFTVLPFVDMIIALKLLIVVVWVGAGVSKFGKHFANVIPPMVSNSPSMPFKWIKRA
                     HYRDFPHDLRPSRVADIMAHVAGTTVEIITPMVLLFSTNKWLTVSAVALMVVFHLFIV
                     STFPLAVPLEWNVLFAYASIFLFLGFPAWDGYAVTDMSSPWLTALIVAALVFFPILGN
                     FRPDKVSFLPSMRQYAGNWASAVWAFSPGAEAKLNKVTRTAKNQVDQFVEFGYEPQWA
                     EITMQSTIAWRTMHSQGRGLFSVLLKTLPDIDTRTVREAEFTCNSLIGFNFGDGHLHN
                     EDLIAAVQREAAFEPGELVVVWVESQGWGSKVQHYKVIDAALGVIERGTWKVADAVAE
                     QPWLPNGPIPTEVAWSLVTERDRVEQTLNRGTHNNGHGALA"
     gene            3274..4692
                     /locus_tag="VGP27_28340"
     CDS             3274..4692
                     /locus_tag="VGP27_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7855239.1"
                     /translation="MNTTATVVGAGPNGLAAAVTLAKAGMQVTVLEAADEIGGGTRSS
                     EAIIPGLLHDHCSAIHPMAVGSAFLNDFGLDRYGLTWRLPEIDCAHPLDDGTAGVLYR
                     SVGSTADGLGADGWKWRALFDRPSKSFDALSEDIMGPLLRVPHHPLTLARFGAPTVLP
                     GSALARWFRTGEAQALFGGVAAHAFRPLHYPMTSAIGLGIITAGHRHGWAVASGGSQS
                     ITNAMAALLTDLGGKVETGTRIEAASQLPPADVTMFDLAPEAVAGILGDRLPRQVARA
                     YRRFRRGPGAFKVDFAVEDGVPWTNSDARRAGTVHVVGPFAELAATERDIHAGRMPER
                     PFVLVGQQYLADPKRSVGNTHPVWAYAHVPNGYAGDATAAIIDQIERFAPGFRDRIAG
                     HTVRSTTQMASYNANFVGGDIMTGSKDIRQLSFGPRITLSPYRVGIPGMYICSAATPP
                     GPGAHGMCGYNAAQSALAHLTH"
     gene            4720..5889
                     /locus_tag="VGP27_28345"
     CDS             4720..5889
                     /locus_tag="VGP27_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7855240.1"
                     /translation="MSMTAIDTSFDLFQLTDEHHELRAAIRALTERDIVPHAADCDER
                     ERFPQEALDALNQSGFNAVHVPEAYGGQGADGLATCIVVEEVARGCCSSSLIPAVNKL
                     GTMGLILAGCEDLKQKVLPDIANGAMASYALSEREAGSDPASMRTRARADGDGWILNG
                     TKCWITNGGKSAWYTVMAVTDSDEGANGISAFMVHKDDEGFSVGPKERKMGIKGSPTA
                     ELYFENCYVPGDRIIGEPGSGLKTALMTLDHTRPTVGAQAVGVAQGALDVAVAYTKDR
                     KQFGKSISSFQAIQFELADMAMKIEAARLMVYTAAARAHRGETNLAFISAAAKCFASD
                     VAMDVTESAVQLLGGAGYTRDFPAERMMRDAKITQIYEGTNQIQHIVMSRALLAG"
     gene            complement(6118..>7034)
                     /locus_tag="VGP27_28350"
     CDS             complement(6118..>7034)
                     /locus_tag="VGP27_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV7855241.1"
                     /translation="VDIEEPPPPGPHEVLVSVEVSPLNKHDLLVVSGVLARPPLPHIP
                     GAEGVARVLATGAEVDGLQVGDLVVLPLYAGAWRERLVVPADSLFALPAGGDIEQYSM
                     LGSNPPTAGLMLSEYTSLQPGDWVVQNAANSGVGRSLIALAKLRGLKTINLARDDATF
                     AELTAAGADVVHADDPDAVGDVRAVIGDARVALAVDSVGGPGVARLLELLSDGGRLVG
                     YSWASGEPMSVDTLALVGKHLTVHGFFVGDFHYRDKVVPVIREAAPLVADGALVVPVA
                     GVYPLENIQDAVSHQLRGGKILLKVAGR"
BASE COUNT         1296 a         2303 c         2176 g         1259 t
ORIGIN      
        1 gcgcccgccg tcgccgacga caacatcagc ctgaacaacg gcgagttcat ggaggccgtg
       61 cggcgcgcca gcgcggcgct ccaccgccat ggggtggcgg acggtgacgt ggtggcggcg
      121 atgctgccca acaccaccgc cctcgtcgtt actctgttcg ccgcctggcg gctgggtgct
      181 gcggtcacgc cgatcaatcc ggcgctgcaa ccggtggaag tcggctatca actcgccgat
      241 gctgacgcca aggtgttgat tgtcgacacc gtgccgaact tcgatatcgg cgatcgggcg
      301 gtggtcaccg cgagcgagtt gacgtcgaca ccggccaccg ggcctctgcg gcccgcacac
      361 cttgcggacg acgcgctggc actgctgatc tacacaagcg ggaccacggg tcggcccaag
      421 ggcgtcatgc tggatcactg caatctgatc gccatgtgcc gcggcgtcat cgacgcggtc
      481 gcactgacga gcgccgacca cagcctgctg atcctgccgt tgttccacgt caacgggatc
      541 gtgatcagca ccctcgcgcc acttctggcc ggtggcagga caacgatcgc cggccgattc
      601 agcccgaaga cgtttttcga caggatcgaa caatgcggtg cgacgtattt ctcagccgta
      661 ccaacgattt atacgatcct gagtggtctg cccgcggcag ttaagcccga cacgtcttcg
      721 gtgcggttcg ccatctgcgg tgccgctccg gccagcatcg aactgctcga gcagttcgaa
      781 agccgctacg gcatcccgat cgtcgaggga tacgggttgt ccgaagggac atgcgcaagc
      841 acactgaatc cccttgtcgg ccagcgcaag gccggcacgg tgggactccc tttgcccggc
      901 cagaccattc ggatggtcga ctcgaacggc gcgccggtga gctctggtga agcgggagag
      961 gtcgtcatca aggggccgaa cgtgatgcgt ggctacctca accgccccga agaaaccgag
     1021 aaaaccattg tcgacggctg gttacacacc ggcgacgtcg ggcgcttcga tgaggacggc
     1081 tacctggtgc tcgtcgaccg tgccaaggac atgatcatcc gcggcggtga gaatatctac
     1141 ccccgcgaga tcgaaaccgt ggttcaccag ctgccggaga tcgccgaggc tgccgtcgtc
     1201 gcccggtcga atcccgtcta cggcgaggag ccggtgttgt tcgtctcact gcaccccgac
     1261 aggcagctcg gagtggacgt gatccgcgcc cacctcgccg agtcgctatc gaaatacaaa
     1321 cggcccgttg acatcacgat cctcgatgac cttcccaaga acgccgtcgg caagatcgcc
     1381 aaacccatat tgcgacaacg gctaacccca acccactgaa ccgacaacta ctcaagacag
     1441 aggagacaat gaaatgggct ttaccaaacc gggtctgccc aacgtcgatc cggatacctt
     1501 cctgcagaag ccgctcatgg agcggatgcg aatcctggcg ctgaactggg gggagaacgg
     1561 gttcggatcg cccaagatga tccacaccgt ctacatcgcg aagttggtgc ttttgtacgc
     1621 cttcggtggc gtcgtcgtcg cgacgctcac ttctgggctt cccgcgtttt ggcatgtctc
     1681 cgagtggtgg aaccagccga tcgtgtacca gaaagtcatc ctgtggacgg tgctgctcga
     1741 agtcatcggt atcgccgggt cgtggggacc gctggccggc aaagtgaagc cgatgaccgg
     1801 cggaatccta ttctgggcgc gtccgggaac catccggctg aggccgtgga agtggatgcc
     1861 gttcaccacc ggcgacaggc gaacctggtt cgacgtcgca ctctatgtcg cactgctgat
     1921 cagcgtcgta gttccgctgc tctcgcctgg tgtgcacagt gattcgctgt cgcgggtgat
     1981 gccagagaac acttccggtt tagtcagccc tgcactgctg atcacgccga tcgcgctcct
     2041 ggtggcgatg ggattgcgcg acaagaccgt cttcctcggc gcacgcggtg agcagtatct
     2101 accagcactg gtcttgttca ccgtgctgcc attcgtcgac atgatcatcg cgctcaagct
     2161 gctgatcgtg gtggtctggg ttggtgccgg ggtgtcgaag ttcggcaagc acttcgccaa
     2221 cgtcattccg ccgatggtga gcaacagccc gtcgatgccg ttcaagtgga tcaaacgtgc
     2281 ccactatcgc gacttcccgc acgacctacg gccatcacgc gtcgccgaca tcatggccca
     2341 cgtcgcggga accacggtcg agatcatcac cccgatggtt ctcctgttct cgacgaacaa
     2401 atggctgacg gtttcggcgg tggcgctcat ggtcgtgttc cacctgttca tcgtctcgac
     2461 cttcccgctg gcggttccgc tcgagtggaa cgtactgttc gcctacgcca gcattttcct
     2521 gttcctgggt ttcccggctt gggacggcta cgcggtgaca gacatgtcct cgccgtggct
     2581 cacagcgctg atcgtggccg cgctggtctt cttcccgatc ctgggcaact tccggcccga
     2641 caaggtgtcg ttccttccgt caatgcggca atacgccggc aactgggcct cggcggtatg
     2701 ggccttttct ccgggcgcgg aggccaagct gaacaaggtc acccggaccg caaagaacca
     2761 ggtcgaccag ttcgtcgagt tcggctatga gccacagtgg gccgaaatca cgatgcagtc
     2821 gacgatcgct tggcgcacca tgcacagtca gggccgaggc ctgttctcgg tgctcttgaa
     2881 gacactgccc gacatcgaca cccgcaccgt ccgtgaggcg gagttcacct gtaactcact
     2941 gatcgggttc aacttcggtg acgggcacct acataacgaa gacctcattg ccgccgtgca
     3001 gcgcgaagcg gccttcgaac cgggcgagct cgtcgtcgtc tgggtggagt cacagggctg
     3061 gggcagcaag gtccagcact acaaggtgat cgacgcggct cttggagtga tcgagcgtgg
     3121 gacgtggaag gtggccgacg cggtcgccga gcagccgtgg ctacccaacg gcccgatccc
     3181 caccgaggtc gcctggtcac tggtcaccga acgggaccgc gtggaacaga ccctgaaccg
     3241 gggtacgcac aacaacggtc acggagcgct cgcatgaaca ccaccgcaac cgtcgtgggg
     3301 gccgggccca atggcctggc tgccgcggtg acgctcgcca aggcaggcat gcaggtcact
     3361 gtgctggaag cagccgatga gatcggcggc gggacccgca gcagcgaggc gatcatcccc
     3421 ggcctgctgc atgatcattg ttccgcgatc catccgatgg ctgtcggttc ggctttcctc
     3481 aacgatttcg gtctggatcg ttacgggctg acgtggcggc tacccgagat tgattgtgcc
     3541 cacccgctcg acgacggcac cgccggtgtg ctctaccgct cggtcgggtc caccgctgac
     3601 ggcctcggcg ccgatgggtg gaagtggcga gcgttgttcg accgaccgtc gaagagcttt
     3661 gacgcgctgt ccgaagacat catggggccg ttgctacgag tgccgcacca cccactgacg
     3721 cttgcccggt ttggtgcgcc gactgtgctg ccgggttctg ccctggcgcg atggtttcgt
     3781 accggcgagg cgcaggcgct gttcggaggc gttgcagcgc acgccttccg gccgctgcat
     3841 tatccgatga cgtcggccat cgggctcggg atcatcaccg ccggacatcg ccacgggtgg
     3901 gcagttgcgt ccggggggtc gcagtcgatc accaatgcga tggcggcgct gttgaccgac
     3961 cttggaggca aggtcgaaac cggcacgcgc atcgaggcgg cgtcacaact gccgccggcc
     4021 gacgtgacga tgttcgactt ggcccccgaa gccgtcgcgg gcatcctcgg cgatcgtcta
     4081 ccgcgacaag ttgcacgcgc ctaccgcaga ttccgccgtg gccctggggc gttcaaggtc
     4141 gacttcgccg tcgaggacgg tgtcccctgg accaactctg acgcccgccg cgcaggcacg
     4201 gtgcacgttg tcggcccctt tgccgaactc gccgcgaccg aacgcgacat ccacgcaggg
     4261 cgcatgccgg agcggccttt tgtcctagtc ggacagcaat atctggccga tcccaagcga
     4321 tccgtcggca acactcatcc ggtgtgggcc tacgcgcacg ttcccaacgg ctacgccggt
     4381 gacgccaccg cggcgatcat cgaccagatc gagcggttcg cgccggggtt ccgtgaccgc
     4441 atcgccggcc acaccgtccg ctcgacaacg cagatggcct cttacaacgc caatttcgta
     4501 ggcggcgaca tcatgacggg cagcaaggac atccgccaac tcagcttcgg accacgaatc
     4561 acgctgtcgc cctaccgcgt cggcataccg ggcatgtata tctgctcggc agccacgcca
     4621 cccggccccg gcgcgcacgg catgtgcggc tacaacgccg cacagagcgc gctcgcacat
     4681 ctaacacact gaccacaacc accaccgctc attaaggaga tttccatgac cgccattgac
     4741 acctcgttcg atttgttcca gctgaccgac gagcaccacg aactgcgcgc cgcgattcgc
     4801 gcgctcaccg agcgcgacat cgtgccgcac gcggctgatt gcgacgaacg agaacgattc
     4861 ccgcaggaag ccctcgatgc gctcaaccag tccggcttca atgccgtcca cgtcccggag
     4921 gcctacggcg gtcaaggagc cgacggtttg gccacctgca tcgttgtcga ggaggttgcc
     4981 cgcggatgct gctcctcttc gctgattccg gccgtgaaca agctcggcac gatggggctc
     5041 atcctcgccg gttgcgaaga cctcaaacag aaggtcttgc ctgacatcgc gaacggcgca
     5101 atggcctcgt acgcactgtc cgaacgagag gccggatcgg atccggcaag catgcgcacc
     5161 cgcgcccgcg ccgacggcga tggatggatc ctcaacggca cgaagtgttg gatcaccaac
     5221 ggcggcaagt ccgcctggta caccgtgatg gccgtaaccg actccgatga aggcgccaac
     5281 ggtatatccg cgttcatggt gcacaaagac gacgaagggt tcagcgtcgg ccccaaggaa
     5341 cgaaaaatgg gtatcaaagg ctcccccacc gccgagctgt actttgaaaa ctgctacgta
     5401 cccggcgacc ggataatcgg cgaacccgga tccggcctga agacggcgct gatgacactt
     5461 gaccacaccc gccccaccgt cggcgcccaa gctgtcggcg tcgcccaggg ggcgctcgac
     5521 gtggcggtcg cctataccaa ggatcgcaag caattcggca agtcgatatc cagtttccag
     5581 gccatccaat tcgaactcgc ggacatggcg atgaagatcg aggcggcaag gctgatggtg
     5641 tacaccgcgg cggcccgggc acatcggggc gagaccaacc tcgcctttat ctcggcggcc
     5701 gccaagtgct tcgcgtcgga cgttgccatg gacgtcaccg agtcggcagt acaactgctc
     5761 ggcggtgcgg ggtacacccg tgacttcccg gccgagcgga tgatgcgcga cgccaaaatc
     5821 acccaaattt acgaaggcac caatcaaatt cagcacatcg tgatgtcgcg cgccctattg
     5881 gccggctgag ccagaatcag aaccggttcc tctgactcac cggggcggtt cacgttggtc
     5941 atagaacgtg cgggcgccgg ggctggccga cagagcgcat aggcacattt atcgatggcg
     6001 tcatacaagc ggtgatttcg cacgtggcgg gtcagtgctc agctcgtggc gaacaatttt
     6061 tttctcacgt ttgtctcacg aaagtggcga atcttcagcc gcccgctgtg ctcgcgttca
     6121 tcgccccgcc actttcagca ggatcttgcc gccgcggagc tgatgcgaca ccgcgtcctg
     6181 gatattctcg agggggtaca cgcccgctac cgggaccacg agggcgccgt cggctactag
     6241 cggtgccgct tcccgaatga ctggtaccac tttgtcgcgg tagtggaagt cgccgacaaa
     6301 gaatccgtgg accgtgaggt gcttgccgac cagtgccagg gtgtcgaccg acatgggttc
     6361 gccactggcc caggaatagc cgaccaaccg tccaccgtcg gatagcaatt cgagcagccg
     6421 cgccacccct ggcccaccca cggagtccac tgccagcgcc actcgcgcgt cgccgatcac
     6481 tgctcgaaca tcgccgaccg catcggggtc atcggcgtgg accacgtcgg ccccggccgc
     6541 tgtgagctcg gcgaacgttg cgtcgtcgcg ggccaggttg atcgtcttca ggccacgaag
     6601 tttggctagc gcgatcaatg accgccccac accggagttc gcggcgttct gaaccaccca
     6661 gtcaccgggc tgcagggagg tgtattcact gagcatcaac cctgccgtcg gtgggttgct
     6721 gcccagcatc gagtactgct cgatgtcgcc gccggcgggg agtgcgaaca ggctgtcggc
     6781 gggcacgacg agccgctcac gccatgcacc ggcatacagg ggtagcacga ccagatcgcc
     6841 gacctgaagt ccatcgacct cggcgccagt ggcaagcacg cgggcgactc cctctgcgcc
     6901 gggtatgtgg ggcaggggtg gtcgcgccag cacgccgctg acaaccagta ggtcgtgttt
     6961 gttcaacggc gagacctcaa cgctcaccag aacctcatgc gggcccggtg gcggcggctc
     7021 ctcgatgtcg acga
//
LOCUS       DASYLX010001202         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_983397,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001202 DASYLX010000000
VERSION     DASYLX010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983397"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGP27_28355"
     CDS             <1..567
                     /locus_tag="VGP27_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEV7855242.1"
                     /translation="SDPGVVNYAVLGKGSVGNIVGATLRFESTFTDPFQSFYVDDPAC
                     NNWADIGLPEVYLDPDLASFNGASAQESATDMTHFVKQAVGVFATNDAAARAFHRVVD
                     RTVGCSGLTTAMHLDNMTTEVWTFTGGPASATDADWVKQEAGTDRRCFNTTRLRENVL
                     LQAKVCQPGNGGPAVNVLAGAMQNTLGQ"
     gene            712..1995
                     /gene="egtA"
                     /locus_tag="VGP27_28360"
     CDS             712..1995
                     /gene="egtA"
                     /locus_tag="VGP27_28360"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="HEV7855243.1"
                     /translation="MPTDLDEGHPADAAGTELTSANSAALMIGESCLTDGPVGRVGLE
                     IEAHCFDLADPSRRPAWDEITGVIAGIGALPGGSPITVEPGGAVELSGPPLDGVLAAI
                     TAMAADRAVLQSAFAEAGLGLVLLGADPLRPAKRINPGDRYLAMEQFFVASQTGAAGA
                     AMMTSTASVQVNLEAGPQAEWAARVRLAHALGPVMVAIAANSPLLAGKFTGWRSSRQW
                     VWSQLDSARCGPILGTDGEHPASDWARYAMKAPVMLVHPTPEMDAVPVTDWVPFADWA
                     DGRVLLGDRRPTVADLDYHLTTLFPPVRPRRFLEIRYLDSVPDAEWPALVFTLTTLLD
                     DPVAAGIAEEATEPVATAWDRAAQIGLGDRRLHEAATLCVQAAAERAPAEVEESMQRL
                     VRSVEQRRSPADDFSDRVVRKGIATAVTELAQGEL"
     gene            1992..>2614
                     /locus_tag="VGP27_28365"
     CDS             1992..>2614
                     /locus_tag="VGP27_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV7855244.1"
                     /translation="MSSRETLARRLTTARDRTLRLVEFDDAELHRQYHPLMSPLLWDL
                     AHIGWQEELWLLRANDPRQPGMLAPDVERLYDAFANPRAGRVDLPLLPPADARAYCAT
                     VRNKALDSLDALTDDDGEAAFNFGLVVSHENQHDETMLQALNLRSGPPLLGRGAPLPP
                     GRPGVAGTSVLVPGGPFVLGVDAVTEPQSLDNERSAHVVDVPGFRIGR"
BASE COUNT          413 a          894 c          916 g          391 t
ORIGIN      
        1 tccgatccgg gagtggtgaa ctacgccgtt ctgggcaagg ggtcggtcgg caacatcgtc
       61 ggcgccactt tgcggttcga gtccaccttc accgacccgt tccagtcctt ttacgtcgac
      121 gacccggcgt gcaacaactg ggccgacatc ggtctgcccg aggtgtatct cgatcccgac
      181 cttgcgtcgt tcaacggcgc ctctgcgcag gagtcggcga ccgacatgac gcacttcgtc
      241 aagcaggccg tcggtgtgtt cgccaccaac gacgcggccg cccgggcctt ccaccgggtg
      301 gtggaccgga ccgtcggctg ctcgggcctg accacggcca tgcatctgga caacatgacc
      361 accgaggtgt ggacattcac cggtgggcct gcgtcggcca ccgacgcgga ctgggtcaag
      421 caggaggcgg gaacagaccg gcggtgcttc aatacgactc gcttgcggga gaatgttctg
      481 ctgcaggcga aagtctgtca gccaggcaac ggcgggcccg cggtgaacgt gctggcaggc
      541 gcgatgcaga acacactggg gcagtagcgc ccggcctgtc cgggtagccg tcatcggtct
      601 gtcaggccaa aaaacgcgct gcacacagat ttttgtcagt ccgctctgca agatggagag
      661 aaggccgcga ccgctgcaga cccgagaggg atggcaggta tgaccaacac catgccgacc
      721 gacctggacg aaggacaccc agccgacgcc gccgggaccg agttgaccag tgccaacagt
      781 gctgcgctga tgatcggcga gtcgtgtctc accgacggcc ccgtcggccg ggtcgggctg
      841 gaaatagagg cgcactgctt cgatctcgct gatccgtcgc gtcggcccgc atgggacgag
      901 ataaccgggg tcatcgcggg aatcggagcc ctgcccggcg gaagcccgat cactgtcgag
      961 cccggcgggg cggtggaact gtccgggccg ccgctcgacg gcgtgctggc cgcgatcacc
     1021 gccatggccg ccgaccgggc agtactgcag tcggcgttcg ccgaggccgg tctgggtctg
     1081 gtgctgctcg gcgccgatcc gctgcggccg gccaaacgca tcaaccccgg cgaccgctac
     1141 ctcgccatgg agcagttctt cgtggccagc cagaccggcg cagccggggc ggcgatgatg
     1201 acgtcgacgg cgtcggtgca ggtcaatctc gaggcagggc cacaggccga atgggcagca
     1261 cgggtccggc tggcgcacgc gctgggcccg gtgatggtcg cgatcgcggc caactcgccg
     1321 ctgctcgccg gcaagttcac cggctggcga agttcgcggc agtgggtgtg gagtcagctg
     1381 gactcggctc ggtgcggccc gatcctcggc accgacggcg agcacccggc aagcgactgg
     1441 gcgcgctacg cgatgaaggc ccccgtcatg ctggtccacc ccacgccgga gatggacgcg
     1501 gtgccggtga ccgactgggt gccgttcgcg gactgggccg acggccgcgt gctgctcggc
     1561 gaccgccgtc ccaccgtcgc cgacctggac taccacctga cgacgctgtt cccgccggtg
     1621 cgaccgcggc ggttcctgga gatccgctac ctcgacagcg tgcccgacgc ggagtggccc
     1681 gctctggtct tcaccctcac cacgctgctc gacgatcccg tcgcggcagg catcgccgaa
     1741 gaagccaccg aacccgttgc gacggcatgg gaccgtgccg cacagatcgg gttgggcgat
     1801 cggcggctgc acgaggccgc gaccctctgc gtgcaggcgg cggccgaacg ggcccctgcc
     1861 gaagtcgagg aatccatgca gcggctggtg cgttcggtgg agcagcgacg cagtccggcc
     1921 gacgacttct ccgaccgggt ggtgcgcaag gggatcgcga ccgcggtcac cgaactggca
     1981 caaggggagc tgtgagctca cgcgagactc tggcgcgccg gctgacaacg gcgcgtgacc
     2041 ggacgcttcg gctggtcgaa ttcgacgacg ccgaactcca ccggcagtac cacccgctga
     2101 tgagcccgct gctgtgggac ctcgcgcata tcggctggca agaggagctc tggctgttac
     2161 gcgctaacga cccgcgtcag ccgggcatgc tggcgccgga cgtcgagcgg ctctacgacg
     2221 cgttcgccaa cccgcgggca ggccgcgtcg acctgccgct ccttccgcct gccgatgcgc
     2281 gcgcctactg cgcgacggtg cgaaacaagg cgctcgacag cctcgatgcg ctgaccgacg
     2341 acgacggcga ggcggcgttc aatttcgggc tggtggtcag ccacgagaac cagcacgacg
     2401 agacgatgct gcaggccctc aatctgcgat cggggccgcc gctgctgggc cgtggggctc
     2461 cgcttccgcc gggcaggccc ggtgtggcag gcacgtcggt gctggtgccc ggcggcccgt
     2521 tcgtcctcgg cgtcgacgcc gtgaccgaac cgcagtcgct cgacaacgag cgctcggcgc
     2581 acgtcgtcga tgtgcccggc ttccgcatcg gccg
//
LOCUS       DASYLX010001203         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_983431,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001203 DASYLX010000000
VERSION     DASYLX010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983431"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VGP27_28370"
     CDS             <1..185
                     /locus_tag="VGP27_28370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855245.1"
                     /translation="VYKQTLDGLRLLGRIDWVTEKLQEHRREKPVTIVVDGFTSRRQR
                     WSGHGSKNNRHTGRQV"
     gene            493..969
                     /locus_tag="VGP27_28375"
     CDS             493..969
                     /locus_tag="VGP27_28375"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV7855246.1"
                     /translation="MGSTGDDMSDQLRQVAQDDGGGPAWDRLEDQLGWYDRKSVAAQQ
                     AYKRVKLSQLIVGAAVPVVAALQVSAAVTATLAAFVVVAEGAQQLYQWQTNWVLYRAT
                     AEALKHEKYLYLAAAGPYSAGDRHRVLAERLEGLVSQEHAKWTEAWQKDSHTSSAS"
     gene            complement(1347..2924)
                     /locus_tag="VGP27_28380"
     CDS             complement(1347..2924)
                     /locus_tag="VGP27_28380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855247.1"
                     /translation="MAQVIEYPLDKLASTEVYSLEQDPQGKVENKFTIAAVPYARGLV
                     VNYGGVDTVLGVFVRGSRVFLIEVVTPRAAKASEADIEFTRRLVMIQAAKAPPGPTKL
                     RNLGAPWLAAGTALGTLGAAVAYIGGLSLLAWLRDPLRRRLTSASPQPIALPNITTVD
                     IGARARRRRRRTAAGLVLELSGTGALVAGLVLLPSIWPLGLLLVALGVGMARIPRLVT
                     AAARSATFESKLWTGRRRVRVNCYAAISTVCVLTGLLLMVLYGAGAVLTSPVYHYNIR
                     FFFVCAVLLTVGALIRRHARRLAALDADEVLRRDSRPMVLYLRTFADDSLTIRTAPYA
                     RQSFADRLSPRRFERFEEVLVRNLTEAGPVVALNPPGTDLAPIGAARETLTADHWQST
                     ITGWMTDAQLIVVGAAPEAISPGFGWELRAVDSHRLWFKTLLVLPPVPNSTMHARWER
                     FTKVFADTAMARHPIPLDPASMLVLLGSVATGWSAVTADRRTEWTYAEALAVAITKMT
                     SVGKDQAVSRPSDVGDQ"
BASE COUNT          876 a         1259 c         1380 g          815 t
ORIGIN      
        1 gcgtctataa gcaaacgctg gacggccttc gtcttctggg acggattgac tgggtcacgg
       61 agaaactgca agagcatcgg cgggagaaac ccgtgacaat tgtggttgac ggcttcactt
      121 ctcgccggca gcggtggtcc ggacacgggt cgaagaacaa tcgccacaca gggcgacagg
      181 tgtgaaccgg gcaacccgac cgctgcctag aacatcatga ctgcacacca cgagctctca
      241 ccggcatcca acaagttggt gcgaacgctg atcgggccgt gcgtccgctt gtgcaggagt
      301 ctcgcgacgt gctgttgatc cgcagctacg ttccagtgag aatcgactcg ccgggagcct
      361 caccgttgcc cgtgttgcaa ccaccgcaag gagcccaagc aacccggtcc cacccagatc
      421 actgagtgca tccgccccgt gcgcgcgagc accccatata caagtggttt gtcggggtga
      481 gactctcacc gcgtgggatc gacgggagat gatatgagcg accagcttcg gcaagtagct
      541 caggacgacg ggggcggccc ggcgtgggat cggctggagg accagctcgg ttggtatgac
      601 cgcaagagcg ttgccgccca gcaggcctac aagcgggtca agctcagcca gctcatcgtc
      661 ggcgccgcgg taccggtcgt cgccgccctc caagtgtccg ccgcggtcac cgcgacactt
      721 gccgccttcg ttgtcgtcgc cgagggcgcc caacagttgt atcaatggca gaccaattgg
      781 gtgctgtacc gcgccaccgc agaagccctt aaacacgaga agtacctcta tcttgccgcc
      841 gccggtcctt acagcgcagg tgaccggcac cgtgttctgg cagaacgcct cgagggtttg
      901 gtttcacaag agcatgcaaa atggacggaa gcgtggcaaa aagacagtca cacttcttca
      961 gcgagctagt gtcccgtaag cgtgtcccct gcagtactaa atgaaaaagg ccgagtcttc
     1021 gcggaggccg tgacgtgata ccgggggtat ggttcgaact tgtgacgtct gggtcatgag
     1081 gtgagtccga ccgtcgcaca cctgtcatct gcgcagggaa atcagcgcaa agcatgccga
     1141 gccccaaggg cagcagggaa tgctggtctc ctagcagggt cagacacgga ctgacggatg
     1201 accgcttgga ctgcagacca gcatcggtcc ggcgaaccaa ggaatagtca accaatgccc
     1261 caactgtctc ggcgaagacc agcgggtcgt tggccactcg gtccaaggaa acgggcaggt
     1321 gattgtcatg gccgtctcag tagttctcac tgatccccga catctgacgg cctggaaaca
     1381 gcctgatctt tcccgactga ggtcatcttg gtgatagcta ctgccaaggc ctctgcgtag
     1441 gtccactcgg tccgccgatc cgcggttacg gcggaccacc ctgtagctac agagccgaga
     1501 agcacgagca tgcttgccgg atctaaggga atggggtggc gtgccatggc agtgtctgcg
     1561 aacacctttg tgaaccgttc ccatcgcgcg tgcatcgtgc tattaggtac cggcgggaga
     1621 acaagcagtg ttttgaacca cagccggtgg gagtcgaccg cgcgtagctc ccatccgaat
     1681 cctggcgaga tggcctcggg ggcggcccca acaacaatga gctgtgcatc ggtcatccat
     1741 ccggtgatgg tggattgcca atgatcggca gtcagcgtct cgcgtgctgc gccaattggc
     1801 gcaaggtcgg tgccgggtgg gttgagggcg acaaccggtc cggcctcggt gaggttccta
     1861 accaggacct cctcgaaccg ttcgaaccgg cgcgggctga gccggtcggc gaaagattgg
     1921 cgggcataag gggctgtgcg gatagtaagc gagtcgtccg caaatgttcg tagatacaac
     1981 accatcggcc gagaatcgcg tcgtaacacc tcgtcggcgt cgagagcggc caggcggcgt
     2041 gcgtgacggc ggataagggc cccgactgtc aggagcacag cacagacaaa gaaaaagcgg
     2101 atattgtaat ggtatacagg agaggtcagg actgctccag cgccgtacag gaccatgagg
     2161 agcaggccgg tgagcacaca gacggttgag attgccgcat agcagttgac ccgaacccga
     2221 cgccgccccg tccatagttt cgattcgaag gtcgcactgc gagccgctgc tgtgacgagt
     2281 cggggtatcc gggccatgcc aacgccgagc gcgacgagga ggagtcccag tggccagata
     2341 gacggtaaga gcacgagccc agccaccaac gctccggtgc cgctgagttc gagcaccagc
     2401 ccagccgcag ttcgccgacg ccgacgtcgg gcgcgtgccc caatgtcaac ggtcgtgatg
     2461 ttcggcagcg cgatcggttg aggtgacgca ctggtcaggc gccgtcgtag cggatcgcgg
     2521 agccatgcga ggagtgacag gcccccgata tacgccacag ccgcgccgag cgtcccgagt
     2581 gcggtccccg ccgcgagcca tggtgcacca aggttgcgaa gcttcgttgg gcccgggggt
     2641 gccttggccg cctggatcat tacgagccgg cgggtaaact cgatgtcggc ttccgatgct
     2701 ttggccgctc ggggggtgac gacctcgatg aggaagacac gcgaaccacg gacgaagacg
     2761 ccgaggacag tgtctactcc gccatagttg acgactaaac cacgtgcata gggcacagcc
     2821 gcgatggtga atttgttctc tactttacct tgcgggtctt gctctaaaga atatacttcg
     2881 gtgctggcca gcttatcaag aggatattcg atgacctgtg ccatcagtac tctgcgttcc
     2941 gatcgattaa atccagaaac gttgataatt acggatgtgt gtggcgtgct gcgtaatcgg
     3001 cgaaagccag ctaggtccag gtagcggttc gtcgtggaat ccggattgct tcggatcaag
     3061 ttcgaaacct ggtgggacgt caccgctgtt gagtaagaac gcaccaaggt ccgactctgg
     3121 ggattggcta cccagtgcgg ccgccgcgac ggacggcgtt gctatgagcc acgtagctgc
     3181 gaaaatacac gcgccaacgg ctaacacaat acgccgccag aagctcatga tggctcccat
     3241 aaggattgtc gaactagtca tcaatgacgc atcggagtcc ggagggtgca gtgagtggcg
     3301 aagcggccgt gcaacacgcg actgcggtgt ttctaaacat cgagcatcaa ctcatcgcct
     3361 cgtgggtacc gggctggcca ccctgagcga ttaacaggac gtaggcgctc gctggtcagg
     3421 gtcgaggttt gcgtggagca tgcgatgcac tgcttcaccg gtgtagatct cggtggcatc
     3481 caagtaccgg ccgcaggcat ccagcacggc ggccgggcat gcacctttgt gtgcgtcaga
     3541 tcaagcttga acgcgtcgag gctcctcaca gcgcacctcc cgggctgatc ggctgaacgg
     3601 cttcgtgtcg agaccaagat cacagacgtg gtcatcccga gcgtcacagg gatagagcga
     3661 cgggtccacg gcatgcgtac aataaaggtc ccgaagactc gcagttcgtc cctatgaccc
     3721 gcggttagcg agcggacggc gatgcgctga gatcgtgcag atactgtgag tccggcttat
     3781 aggccacctc gtccgtccgc actccgccac tcgccccaat gaggagacgc tccgggttga
     3841 gccggggatg ctctcgctgc ttctccgggc ttctcaacgc tccctgacgt ccgacggccc
     3901 agtggcggcc caggcaacga tcttgtccat tgttccctcg cgtggtgaga gctcgggtgg
     3961 tgatctcctg atgacgacgt gcaccccctt gtgatggcgc ggcgatggtg gcggcgccga
     4021 tgggtgatcg cagcagtggt ctccggacat ttggatctcc tccgcaagag ggtgaccctc
     4081 accagttggc aggaccggct gtggctagga gatgtcgcgc catgagcgct ttcgttaggc
     4141 gtcgcgcggg ccctgcgggc tgatccgaga agatcgactg gcccaacgtc acgatggtcg
     4201 tcaggcctag acccaagttt cgtgatcttg ggcggtttac cctctgttga gctggggatt
     4261 cgttgttgag gccggttttg ttctgtctac ttcatcccag ggtcagtggt actggggcgt
     4321 tgatgagttc
//
LOCUS       DASYLX010001204         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2411260,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001204 DASYLX010000000
VERSION     DASYLX010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411260"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGP27_28385"
     CDS             complement(<1..267)
                     /locus_tag="VGP27_28385"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7855248.1"
                     /translation="MRSMRVVVVGGGIGGLSAALALRAAGADVRVYEQAAVLGEVGAG
                     VGLYPNSMRILQRLGVADAVIRRAAPINEWWILAPGGPVLSHQVA"
     gene            complement(276..1052)
                     /locus_tag="VGP27_28390"
     CDS             complement(276..1052)
                     /locus_tag="VGP27_28390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7855249.1"
                     /translation="MQPRADLYADRARADSFGAAARMYDARRPRYPELLIDDLLSGRA
                     QKVLDVGAGTGIVSEQLLRRGVDVLAVEPDPRMAAIAREKGIPIEIGTFESWACAQRR
                     FDLVVFGSSFHWVNPDIAVPKAHRLLRRGGRLALMWNRLFPTHPTHNDFAEIYRDYMD
                     AGSPLVDGSWDGLVETDRHTDGLNAAIAASGFTVEQRNYPRHDHFSTEHWLDLAFTYS
                     NHLILAPEKAAELRARLAECIGSKGVSVGGDTLLVLATRS"
     gene            1075..>2431
                     /locus_tag="VGP27_28395"
     CDS             1075..>2431
                     /locus_tag="VGP27_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7855250.1"
                     /translation="MVGSAVDLALYSVFRLAGIEREVLAAVRTCDQRQRQPWRDQRPW
                     IYGLYLILHVRTESPAHQAALMMRAVVIGGSLGGLTAALILREQGWDVDVLERSPNPL
                     EGRGTGIVVHPTTVRYLVERAGKRIGDIGLPARRIQYLDADGSIADEQPCAYRFASYV
                     ELYRGLLDLFGTDRYHLSKELADLDNRTDGATVSLTDGQTLAADLVVCADGIRSTGRR
                     LLVPDARPRYAGYVAWRGTVDGGEISDRSAGILLDAFTYRILPHGHLLTYAIPGPRGS
                     ALFNWLWYQNIAPGDRLTDLLTDRDGVRTELSVPPGSVQTRHVDQLYAVASDLPSPLT
                     EVIQQTAKPFIQVIVDLEVPQMAFGRSCLIGDAAFALRPHIGVGTAKAADDAWQLGSA
                     LLGVTAQRVADRLEGWEIQQLSVARRALRRARTAGRSLQDGSWRIGEQPPFGLLAPGD
                     SV"
BASE COUNT          441 a          789 c          770 g          431 t
ORIGIN      
        1 ggcgacctga tgcgacagga cagggccgcc aggggcgagt atccaccatt cgttgatggg
       61 ggcggcacgg cggatgacgg catccgcgac gcccagccgc tgcagtattc gcatgctgtt
      121 ggggtagagc ccaactcccg cgccaacctc gccgagcacg gcggcctgct cgtacaccct
      181 gacgtcggct cccgcggccc gaagcgcaag cgccgccgac aggcccccga taccgccacc
      241 gacgaccacc acacgcatcg acctcatggc acctcctatg agcgggtggc gaggaccaac
      301 aaggtgtctc cgcccaccga cacgcctttc gaaccgatgc actcagccag cctggctcgt
      361 agctcagcgg ccttctcggg ggcgaggatg agatggtttg aatatgtaaa agcgaggtca
      421 agccagtgct ccgtggaaaa gtggtcatgc cgcggatagt tgcgctgctc aactgtgaac
      481 ccggaagccg ctatcgccgc gttcaagccg tcggtgtgac ggtcggtctc gacaagaccg
      541 tcccatgagc cgtcgaccag aggtgatccc gcgtccatat agtcccggta tatctcggca
      601 aagtcgttgt gggtggggtg cgttgggaac aggcggttcc acatcaatgc cagccggcca
      661 cctctgcgca gcagtcgatg ggccttcggt accgcaatgt cgggattgac ccagtgaaag
      721 gacgatccga acacgacgag atcgaagcgg cgctgcgcac aggcccagct ctcgaaggtg
      781 ccgatctcga tgggtatgcc cttctccctc gcaatcgcag ccattcgcgg atccggctca
      841 acggcaagaa catcaacccc tctccgaagc agttgttcgg agacgatccc ggtacccgca
      901 ccaacatcga gcaccttttg ggcgcgccct gacaagagat cgtcgatcag cagctcgggg
      961 tagcgaggcc tacgcgcatc gtacattcgc gctgcggcac cgaatgagtc cgcacgcgca
     1021 cggtcagcgt agaggtctgc gcgtggctgc atactgaaac tctacgctga cgtaatggtc
     1081 ggcagtgcag ttgatttggc tctctattcg gttttccggc tagcgggtat agaacgggaa
     1141 gtgctggcgg cagtgcgcac ctgcgaccag cgccaacgac aaccgtggcg agaccagcga
     1201 ccttggatat atggcctcta cctcatcctc cacgttcgca ccgagagccc tgcccaccag
     1261 gcggccctga tgatgcgggc cgtcgttatc ggtgggtcgc tgggcggact caccgccgcc
     1321 ctgattctgc gggagcaggg ctgggatgtg gacgttctgg aacggagtcc gaatccgttg
     1381 gagggacgcg gaaccggcat cgtcgtgcac ccgaccacgg tccgctatct cgtcgagcgt
     1441 gcgggcaagc gaattggcga catcggcctg cctgcccgca ggattcaata tctcgatgcc
     1501 gacggctcaa tcgccgacga gcagccatgc gcataccggt tcgcatcgta tgtggagttg
     1561 tatcgagggt tgctggatct attcggcacc gatcgctacc acctctcgaa agagcttgcg
     1621 gacctggata accggacaga tggcgccaca gtgtcattga ccgacggtca gacacttgca
     1681 gccgatctcg tcgtatgtgc cgatgggatc cgttccaccg gacgccgact actggttcca
     1741 gacgcccgac cccgctacgc ggggtatgtg gcatggcgcg gcactgtcga cggcggcgaa
     1801 atcagcgacc gctcagcggg cattctgctg gacgcattca cctaccggat cctcccccac
     1861 ggccacctgc tgacctacgc gattccgggt ccgcgcggct ctgcgctttt caactggctc
     1921 tggtatcaga acatcgcgcc gggcgatcgg ctcacggacc tgctcaccga ccgcgatggt
     1981 gtcaggacag agctcagcgt gccccccgga tcggtgcaga cccgccacgt cgatcagctg
     2041 tatgccgtgg cgtctgatct gccttcgccg ctcaccgagg ttatccaaca aaccgccaag
     2101 ccgttcatcc aggtgatcgt cgaccttgaa gtgccccaga tggccttcgg gcggagctgc
     2161 ctcatcggcg atgccgcgtt cgcgttgcgg ccacacatcg gtgtcggtac cgcgaaggct
     2221 gccgacgatg cgtggcagct ggggagcgcg ctgctcggcg tcacggcaca gcgggtcgca
     2281 gatcggctgg agggctggga gattcaacag ttgtcggtgg cgcggcgcgc cctgcgcagg
     2341 gcccgcacgg ccggtcgcag cttgcaggac ggcagctggc ggatcggcga gcagcccccg
     2401 ttcgggctgc tcgcgcccgg tgacagcgtt c
//
LOCUS       DASYLX010001205         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2958947,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001205 DASYLX010000000
VERSION     DASYLX010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958947"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGP27_28400"
     CDS             <1..217
                     /locus_tag="VGP27_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7855251.1"
                     /translation="IAGVSNTVAALVFAFTAPVQWLAAIALGAGCLVGSRLGPIVVRH
                     APAGPLRALIAAAGVALAVKLGFDAYR"
     gene            complement(214..1281)
                     /locus_tag="VGP27_28405"
     CDS             complement(214..1281)
                     /locus_tag="VGP27_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV7855252.1"
                     /translation="MPDDAQPLPTLSEADQGALQRWLRQQGLGSTVTDVEPLTGGTQN
                     IVVRVHVDGRPMVLRRPPLHPRPTSDKTMLREIAALRTLAGTDVPHPGFIAGCDDLAV
                     LGVVFYLMEEIDGFNPGSDMSEAYVRDEGMRHDVGLSYAAGLAKLGNVAWDGKPLAEL
                     KRPGSFVERQVPQFLRLLESYRHDRYDPATLGVADLAEWLQSNLPREGEPGIMHGDPH
                     LSNVLLRRDKPELAAFVDWEMCTIGDPLLDLGWVLITWPLNTNAITAGAALAALGGLA
                     SRKELLDAYWAAGGRQTSRLDWYTAMACFKLGIVIEGTWSRYLIGKASRDAGEALHFN
                     AQNLIEMGTRVAKGDSPFDLG"
     gene            complement(1294..2118)
                     /locus_tag="VGP27_28410"
     CDS             complement(1294..2118)
                     /locus_tag="VGP27_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7855253.1"
                     /translation="MNSQAETVDEAVIAKHRAIWATGDYPKLALELVAPLGPVLVEAT
                     GCGPGDRVLDVAAGTGNAAIPAAKTGATVIASDLCPELLQAGAALASDQGVELEWREA
                     DAHALPFGDNEFDVVMSCIGVMFAPFHEQSAGELIRVCKPGGRLGLLSWTPDGHIGQL
                     FATMKPYMPPPPPGAQPPPLWGREDHVRELLGDRVTDVVTQRRTLTVDHFVDGAEFRD
                     YFKNMYGPTIAAYRNIEGDADRTAALDADIARVGDGFLENSDTMEWEYLLLTARKA"
     gene            2264..>2537
                     /locus_tag="VGP27_28415"
     CDS             2264..>2537
                     /locus_tag="VGP27_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7855254.1"
                     /translation="MSGYGQFCPVAKAMELLDERWTMLVIRELILGSAHFNDLRRGVP
                     KMSPTLLSKRLKRLTRAGVIERGEIDGRTTYSLTPCGKELADVVESL"
BASE COUNT          403 a          825 c          844 g          465 t
ORIGIN      
        1 catcgccggt gtctccaaca ccgttgcggc gcttgtcttc gcgttcaccg ccccggtgca
       61 gtggctcgcc gccatcgcgc tgggcgccgg ttgcctggtc ggatcgaggc ttggtcccat
      121 cgtggtgcgc catgctcctg ccgggccgct gcgggcgttg atcgccgcgg cgggcgtagc
      181 gcttgctgtc aagctgggct tcgacgcgta ccgctagccc aggtcaaagg gactgtcgcc
      241 cttcgcgacc cgggttccca tttcgatcag gttctgggcg ttgaaatgaa gcgcctcgcc
      301 cgcgtctctg cttgccttac ctatcagata gcgcgaccag gtgccttcga tgacgatgcc
      361 gagcttgaaa cacgccatcg ccgtgtacca atccagccgc gatgtctgcc ggccgcctgc
      421 ggcccagtac gcgtcgagca gttctttccg gcttgccaac ccgccgagtg cggctagcgc
      481 agcgcccgcg gtgatcgcat tggtgttcag tggccatgtg atcagcaccc aaccgaggtc
      541 gagtagcggg tcgccgatgg tgcacatctc ccagtcgacg aatgcggcca actccggctt
      601 gtcccgccgg agcagcacat tgctcagatg cggatcgccg tgcatgattc ccggttctcc
      661 ttcccgcgga aggttggatt gcagccactc cgccaggtcg gcaacgccaa gcgtcgcggg
      721 gtcgtaccga tcgtgccgat agctttcgag gagacgaagg aactgcggaa cctggcgctc
      781 gacaaaggat cccggccgtt tcagctccgc gagcggcttt ccatcccacg ccacgttgcc
      841 cagcttcgca aggcccgcgg cgtaggacag acccacgtcg tgccgcatcc cctcatcgcg
      901 gacgtacgcc tccgacatgt cgctgcccgg gttgaagccg tcgatctcct ccatcaggta
      961 gaagaccacc ccgagcacgg cgaggtcgtc gcagccggcg atgaagccgg gatgcggaac
     1021 atcggtgccc gcaagcgtgc gcagcgcagc gatttcacgc agcatcgtct tgtcgctggt
     1081 cggccgtggg tgaaggggcg gtcgccgcag caccattggg cgaccgtcga cgtgcacccg
     1141 cacgacgatg ttctgggtgc cgccggtcag cggctcgaca tcggtgacgg tcgagccgag
     1201 tccctgttgt ctgagccagc gctgcagggc gccctggtcg gcctcgctca gtgtcggcag
     1261 tggctgcgcg tcatcgggca tgaccacatc gtgtcaggcc ttgcgcgcgg tcaacagcag
     1321 gtactcccac tccatggtgt ccgagttctc gagaaagccg tcgccgaccc gggcgatgtc
     1381 ggcgtcaagg gcggcggtcc gatcggcgtc gccctcgatg ttgcggtacg ccgcgatggt
     1441 cggcccgtac atgttcttga agtagtcccg aaactccgcg ccgtcgacga agtgatcgac
     1501 ggtcagggtg cgacgctggg tgacaacatc ggtgactcgg tcgccgagta gctcgcggac
     1561 gtggtcctcg cggccccaca acggcggcgg ctgcgcaccc ggcggaggtg gcggcatata
     1621 cggcttcatg gtggcgaaga gctggccgat gtggccatcc ggcgtccagc tgagcagccc
     1681 aagcctgccg cctggtttgc acacccggat cagctcgcct gcggactgct cgtggaaggg
     1741 cgcgaacatc acaccgatac acgacatgac gacgtcgaac tcgttgtcgc cgaaaggaag
     1801 tgcgtgggcg tcggcttccc gccactccag ttcgacgccc tggtccgagg caagagcggc
     1861 gccggcctgc agcagctcgg ggcacaggtc gctggcgatc acggtggcgc cggtcttggc
     1921 ggcgggaatg gccgcgtttc cggtgccggc cgccacatcc agcacccggt cgccggggcc
     1981 gcatccggtg gcttcgacca agacagggcc gagcggggcc accagctcga gagcgagttt
     2041 ggggtaatcg cccgtggccc atatggcgcg atgcttcgcg atcaccgcct cgtcgacggt
     2101 ctctgcttga gaattcatct tggtttcctc cgggttgagc ttacctgtga agctgctgtg
     2161 agcatcgtcc gccgatctgg cgccctgatc cagtagcaaa tctgtagcgg cgctggttcg
     2221 atatctgtac tggggattgc gcacggagaa cggaggtgcg acggtgtccg gttacggcca
     2281 attctgtccg gtggccaagg ccatggagct gctcgacgag cgctggacca tgctggttat
     2341 ccgtgaactg atactaggta gcgcccactt caacgatctg cgccgcggtg tgccgaagat
     2401 gtcgcccacg ctgctgtcga agcggctgaa gcggctgaca agggcgggag tgatcgaacg
     2461 cggcgagatt gacggccgca ccacctactc gctgacgccg tgcggaaagg aactcgcaga
     2521 cgtggtcgag tcgctcg
//
LOCUS       DASYLX010001206         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2628654,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001206 DASYLX010000000
VERSION     DASYLX010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628654"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGP27_28420"
     CDS             <1..255
                     /locus_tag="VGP27_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEV7855255.1"
                     /translation="KAAAFVSYGSVGGARAVEHLRLICSELQIAHVRQQLSFSMFTDF
                     ENFTSFRPVAAIHDDAATVMFDQLEAWAGALKPLRKEVAQ"
     gene            252..1022
                     /locus_tag="VGP27_28425"
     CDS             252..1022
                     /locus_tag="VGP27_28425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7855256.1"
                     /translation="MRGLQGTVALVAGAAPGNIGAAAAIRLAEEGTSVVAADLNESAA
                     RAVADEIQALGGKAIARSFDITDEASYQELVAVTVNEFGRLDGLFNVAADLSPATLAQ
                     DTDVLSVSLEVWRHTIDVTLSGYMYGIRHALPIMIEQGGGAIVNTMSTSVWMGEDTRV
                     AYQSAKAGLFGLTRHTATLGGKHGVRCNSVAPGVILTGAALANTTEKYRDEILASVRS
                     NRLGAPEDVAAMVAFLFSDDGAYVTGQTILVDGGANFT"
     gene            complement(1061..>2036)
                     /locus_tag="VGP27_28430"
     CDS             complement(1061..>2036)
                     /locus_tag="VGP27_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEV7855257.1"
                     /translation="ARCDTIIKAPSNDPLTAMAIARTVADVAPDHPITKHLAVGYWKG
                     GDLSVEEQLCQPQHIEKIVAWGGLASVKHVTRYIQPGLELIALDPKRSATIIGAEAFE
                     DEATMRDVAGRAAVDIGVANQEGCANARVIYVMSGTDAAGIANANRLGEMIYEALIGL
                     PEFISTPPLHPSRELFEHLEASRMTEDWYHVIGGEHREGAIVVSQFDSPVDYSAMLSG
                     RVANIVPVDGLDKVTAAVNAYTQTIGIYPESLKRQLRDQLPLFGAQRLTTLGYACNVA
                     IAMPQDAIEPIRRMCKWIVDEECDPEVVIPLWRLGVARAGPDADLAQV"
BASE COUNT          336 a          664 c          671 g          365 t
ORIGIN      
        1 aaggccgcgg cctttgtcag ctacggatct gtgggcggag cccgcgcggt cgagcacctc
       61 cgactgatat gcagcgagct acagatcgca cacgtgcgcc agcagctgtc gttctcgatg
      121 ttcaccgact tcgagaactt cacctcgttc cgtcccgtcg cggcgatcca cgacgacgcg
      181 gccaccgtga tgttcgacca actggaagcc tgggccggcg cgctcaagcc gcttcgcaag
      241 gaggtcgcgc aatgagagga ctgcagggca cggtcgccct tgtcgccggc gcggcgccgg
      301 gaaacatcgg cgcggcggcc gccatccgcc tcgccgagga gggcacgtcc gtggtggccg
      361 cagacctcaa cgagtcggcc gctcgggcag tcgccgacga gatccaggcg ttgggcggca
      421 aggcgattgc tcgttccttc gatatcaccg acgaggcgtc ctaccaggag cttgtcgccg
      481 tcacggtcaa cgagttcgga aggctcgacg gcctgttcaa cgtggccgcc gacctctcgc
      541 ccgccacgtt ggctcaagac acggacgtgc tctcggtgtc gctcgaggtg tggcggcaca
      601 ccatagatgt caccctcagc ggttacatgt acggcatccg ccacgcgctg cccatcatga
      661 tcgagcaggg cggcggtgcc atcgtcaaca ccatgtcgac cagtgtgtgg atgggtgaag
      721 acacgcgggt cgcctaccag tcggccaagg cgggactttt tgggctcacc cgccacaccg
      781 ccaccctcgg cggcaagcac ggcgtgcgat gcaactccgt cgcgcctgga gtgatcctca
      841 ccggagcagc gctggccaat accaccgaga agtatcgcga cgagatcctc gcatcggtgc
      901 gatcaaaccg gctcggagcc ccggaggacg ttgcagcgat ggtcgcgttc ctgttctccg
      961 acgacggcgc ctacgtcacc ggccaaacca tcctcgtcga cggcggcgcc aacttcacgt
     1021 gagcccttac actgcgcgtg atcccagctg ctcgctccgg ctagacctgc gccagatcgg
     1081 cgtcggggcc cgcgcgagcc acccctagcc gccagagcgg aatcaccact tcgggatcac
     1141 attcttcgtc cacgatccac ttgcacatgc ggcgaatggg ttcgatggcg tcctgtggca
     1201 tcgcgatggc gacgttgcat gcgtagccga gggtggtgag tcgttgggcg ccgaacaacg
     1261 gaagctggtc gcggagttgg cgtttcaggc tctcggggta gatgccgatg gtctgggtgt
     1321 aggcattgac cgcggcagtg accttgtcga ggccgtcgac aggcacgatg ttggcgacgc
     1381 gaccggacaa catcgctgag tagtccaccg gggaatcgaa ctgggacacg acgatcgcgc
     1441 cctcgcggtg ttcgccgccg atcacgtggt accagtcttc tgtcatgcgt gaggcttcga
     1501 ggtgctcgaa cagctcgcga ctgggatgca gcggaggggt actgatgaac tcgggcagtc
     1561 cgatcagcgc ctcatagatc atttcgccga ggcggttggc gttggcgatg cctgcggcgt
     1621 cggtgccgct catcacgtag atcacccggg cgttggcgca gccctcctga ttggcgacac
     1681 cgatgtccac tgccgcgcga cccgcgacgt cgcgcatggt ggcctcgtcc tcgaatgcct
     1741 ccgccccgat gatggtcgcg ctgcgcttcg ggtcaagggc gatcaactcc agccccggct
     1801 ggatgtagcg ggtgacgtgc ttgaccgacg ccagccctcc ccacgcgacg atcttctcga
     1861 tgtgctgcgg ctgacacagc tgctcctcga cgctcagatc accgcccttc cagtagccga
     1921 ccgcgaggtg tttggtgatg gggtgatcgg gcgcgacgtc ggccacggtt cgtgcgatgg
     1981 ccattgcggt cagcgggtcg ttcgagggcg ccttgatgat ggtgtcgcaa cgggcg
//
LOCUS       DASYLX010001207         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1644303,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001207 DASYLX010000000
VERSION     DASYLX010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644303"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VGP27_28435"
     CDS             complement(<1..1296)
                     /locus_tag="VGP27_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7855258.1"
                     /translation="MSVVVTMVALACGCADAGPAGPAGAAQSIHIDTNTPQGLRAKQI
                     MDMLNSDWPIGPNGIRTLAAPKIMDDVGVTMDKIWWDRPFTLTGLEVGAGHATLHVLT
                     SYQVSQTIELRTNDAGLVDRFDVSLVPPVIKSWKDIDAEISKTGAKYSYQVSKVDDGK
                     CSLVAGTNTDLSLPLASIFKLYVLLAVSEAVKAGGVSWDDQLTVTREGKLVGAAGLDK
                     IPNGTTVSVRTAAQQMISASDNMATDLLINRVGPGAVERALVTAGHHDPASMTPFPTT
                     HELFSVGWGRPNLRDRWQQVSSSSAGRAQVFTETNSRPYDPDPERTHTPASDIGAEWY
                     ASAADICRVHAALQASAVGPAAPVKNILSAIPGIDLDRAKWPYIGAKGGNLPGDLTFS
                     WYAVDRAGQPYVVSFQLNWPKFRSQTAAAWLLSIVHQTFD"
     gene            complement(1328..1645)
                     /gene="mhuD"
                     /locus_tag="VGP27_28440"
     CDS             complement(1328..1645)
                     /gene="mhuD"
                     /locus_tag="VGP27_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobilin-forming heme oxygenase MhuD"
                     /protein_id="HEV7855259.1"
                     /translation="MPVVKINAIEVPPDAGPELEKRFAHRAHAVDNQPGFLGFQLLRP
                     VKGEDRYFVVTQWESEEAFQAWATGPAVEAHAGQRANPVATGASLLEFEVVLDVAGTD
                     AEA"
     gene            1701..3119
                     /locus_tag="VGP27_28445"
     CDS             1701..3119
                     /locus_tag="VGP27_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV7855260.1"
                     /translation="MTRGRLDAFFELTARGSTPTAEIRGGVVTFIAMAYIVVLNPIIL
                     SDAHDVDGHTLNFAQVSAATSLAAGVMTVLFGLIARLPFAFAAGLGINSFLATTVVGS
                     LTWPEAMGLVVINGIIIVVLAATGLRTLIFDAVPMQLKLAITAGIGAFILFIGLVDAG
                     FIGSTGLPSPPVGLGANGAGSISTVPTLVFVCTLLLTCILVVRHVRGAIFIGLVAGTV
                     LAVAIEAIWHLGSSVDHPGGWSLSVPTLSGSPFAMPDLSLVGAFSLDSFGRIGVLAAV
                     MLVFTLVFANFFDAMGTFTGLSREVNVADDRGMFPRLRSALIVEGAGAVVGGVTSSSS
                     NTVFLESAAGIQEGARTGLANLVTGLLFFAAMFVTPLASIVPTEVAAAALVVVGVMMF
                     SHLRDVDLSEFSVAVPVVLTVAVMPLSFSIANGIGIGFIAWVVMQSAAGKFRDVSPLL
                     WIAATGFLVYFARAWIESLIGM"
     gene            3122..>3288
                     /locus_tag="VGP27_28450"
     CDS             3122..>3288
                     /locus_tag="VGP27_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7855261.1"
                     /translation="MDGDLLTRRGGHGDPLVLVHGLMGRGSTWTRQLPWLAEHGEVYT
                     YDAPWHRGRDVA"
BASE COUNT          470 a         1087 c         1090 g          641 t
ORIGIN      
        1 gtcgaacgtc tggtgcacga tcgagagcag ccacgcggct gcggtctgac tgcggaactt
       61 cggccagttc aattggaagc tgaccacgta cggttgcccg gcgcggtcga cggcgtacca
      121 gctgaatgtc aggtcgccgg gtaggttccc gcccttggcg ccgatatacg gccacttcgc
      181 gcgatccagg tcgattcccg gaatcgccga caggatgttc ttcaccggag cggcgggccc
      241 gacggctgaa gcctgcagcg ccgcgtgcac acggcagata tcggccgcag aggcgtacca
      301 ctcggcgccg atgtccgatg ccggcgtgtg ggtccgttcc gggtcggggt cataaggccg
      361 cgaattggtc tctgtgaaca cttgtgcccg ccccgccgaa gacgacgaga cctgctgcca
      421 ccggtctcgg agattcggcc tgccccaacc gaccgagaac agttcgtgcg tcgtcgggaa
      481 cggcgtcatg ctcgccggat cgtgatgtcc cgcggtgacc agcgcacgtt cgacggcacc
      541 ggggcccacg cgattgatca gcaggtcggt tgccatgttg tcgctggccg agatcatctg
      601 ctgagcggcg gtccgcaccg acaccgtcgt accgttcggt atcttgtcga gacccgccgc
      661 gccgacgagt ttgccttccc tggtcaccgt cagctggtcg tcccagctca cgcccccggc
      721 cttgacggcc tccgaaaccg cgagcagcac atacagtttg aagatcgacg ccagcggcag
      781 cgagaggtca gtgttggtcc cggcgaccag ggagcacttg ccgtcgtcga ccttcgacac
      841 ctgataggag tacttcgccc cggtcttgga gatctcggcg tcgatgtctt tccacgactt
      901 gatgaccggc ggcaccagcg acacatcgaa ccggtcgacc agcccggcgt cgttggtacg
      961 cagttcgatg gtctgcgata cctgatagga cgtcagcacg tgtagcgtcg cgtgtccggc
     1021 gccgacctcc agaccggtca gcgtgaacgg gcggtcccac cagatcttgt ccatcgtgac
     1081 gccgacatcg tccatgatct tcggggcggc cagggtccga attccgttgg gtccgatcgg
     1141 ccagtccgaa ttgagcatgt ccatgatctg cttggcacgc agaccctgcg gcgtattggt
     1201 gtcgatgtgg atgctctgcg cggcaccggc aggcccggcc ggtccggcgt ccgcgcaccc
     1261 gcaagccagg gcgaccattg tgacgacgac cgacatcgag gtcgcccagc ggcgcgccct
     1321 gctcgcgtca ggcctcggcg tcggtccccg caacgtccag tacaacctcg aactccagaa
     1381 gcgaagcgcc ggtggcgacg ggattggcgc gttggccggc gtgcgcctcg acggcaggac
     1441 ccgtcgccca cgcctgaaac gcctcttcgg actcccactg cgtcacgacg aagtagcgat
     1501 cttcaccctt gaccgggcgc agcagctgga agccgaggaa accgggctgg ttgtcgaccg
     1561 cgtgtgcgcg gtgcgcgaac cgcttctcca gttccggacc ggcgtcgggc gggacctcga
     1621 ttgcgttgat cttcaccacg ggcatgcggc caaggctacc gcctgcgtat tggccgcccg
     1681 acgcacctag ggttccagtt atgacgcggg ggcgcctcga tgcattcttc gaactgaccg
     1741 cgcgtggctc gacaccgacg gcagagatac ggggcggcgt cgtcacgttc atcgcgatgg
     1801 cctacatcgt cgtgctgaac ccgatcatcc tgtccgacgc ccacgacgtc gacggccata
     1861 ctctgaactt cgcgcaggtc tcggccgcga catcccttgc cgcgggcgtc atgacggtcc
     1921 tgttcggtct gatcgcccgg cttccgttcg cgttcgcggc ggggttgggt atcaactcat
     1981 tcctcgcgac gaccgttgtc ggctcgctca cctggcccga ggcgatgggc ctggtggtca
     2041 tcaacggcat catcatcgtg gtgctggcgg cgacgggcct gcgcacgttg atcttcgatg
     2101 cggtacccat gcagctcaag ctcgcgatca cggcgggtat cggcgccttc atcctgttca
     2161 tcgggttggt cgacgcgggc ttcatcggat cgaccgggtt gccgtcgccg ccggtgggcc
     2221 tcggcgccaa tggcgccggt tcgatcagca cggttccgac gctggtgttc gtttgcacgt
     2281 tgctgctcac ttgcatcctg gtcgtgcggc acgtgcgcgg cgccatcttc atcgggctgg
     2341 tggcaggcac cgtgctcgcg gtcgccatcg aggccatctg gcatctcggg tcgtcggtgg
     2401 accatccggg tggctggagt ctctcggtgc ccaccctgtc gggctcgccg ttcgccatgc
     2461 cggacctctc gctggtcggc gctttcagcc tggacagttt cggtcgaatc ggcgtgctgg
     2521 ccgcggtcat gctcgtgttc acgttggtct tcgcgaactt cttcgacgcg atgggcacct
     2581 tcaccgggct ttcgcgcgag gtgaacgtcg ccgacgaccg cggcatgttc ccgcggctgc
     2641 gctccgcgct gatcgtcgag ggcgccggtg cggtcgtcgg cggggtgaca tcgtcgtcgt
     2701 ccaacacggt cttcctcgag tcggcggcgg gcattcagga aggtgcgcgc acgggattgg
     2761 cgaacctcgt cacgggactg ctgttcttcg cggccatgtt cgtgacgccg ctggcgtcga
     2821 tcgtgccgac ggaggtcgca gcggcggcgc tggtcgtcgt cggcgtgatg atgttctcgc
     2881 accttcgtga cgtcgacctc tcggagttct ccgttgcggt gccggtcgtg ttgaccgtcg
     2941 cggtcatgcc gctgtccttc tccatcgcca acggcattgg aatcggcttc atcgcatggg
     3001 tggtcatgca atcggcggca ggcaagttcc gtgacgtcag cccgctgctg tggatcgccg
     3061 ccacagggtt tttggtctac ttcgcgcgcg cctggatcga atcgctgatc ggcatgtgac
     3121 cgtggacggc gaccttctca ctcgtcgagg cggtcacggc gacccgctgg tgctcgtgca
     3181 cggcctgatg gggcgcggca gcacatggac gcgccaactg ccgtggctgg ccgagcacgg
     3241 tgaggtctac acctacgacg cgccgtggca ccgcggccgc gacgtcgc
//
LOCUS       DASYLX010001208         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3616730,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001208 DASYLX010000000
VERSION     DASYLX010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616730"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGP27_28455"
     CDS             complement(<1..260)
                     /locus_tag="VGP27_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HEV7855262.1"
                     /translation="MSSTATAASLTAAASPAPPAPLARRWLWLVAAVSLALTFVQSPG
                     KISPDTKLDLTANPLRFLARALNLWNSELPFGQAQNQAYGYL"
     gene            complement(235..408)
                     /locus_tag="VGP27_28460"
     CDS             complement(235..408)
                     /locus_tag="VGP27_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2613 domain-containing protein"
                     /protein_id="HEV7855263.1"
                     /translation="MDRFLVPAAASIVVGLLLGAAAIFGITLMVQQDSKPPVQAGDPA
                     SAVLNRVEYGDRS"
     gene            469..>1532
                     /locus_tag="VGP27_28465"
     CDS             469..>1532
                     /locus_tag="VGP27_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV7855264.1"
                     /translation="MALPRVLGALAALTGLLLACSPATEKPAASPSSGPSNTSLGRPL
                     PGAPVPPACGDVLASMSTRDKLAQLLMVGVTGADDARAVVDNQHVGGIMIGSWTDLSM
                     MGAPLADIAASAGPLPLAVSTDEEGGRVSRLADMIGEQPSPRVLSQTKTPAEVHDIAL
                     QRGQAMRGLGVTIDFAPVVDVTTESDDEVIGDRSFGSDPAQVVEFAGAYAQGLRDAGV
                     LPVLKHFPGHGHASGDSHEGGVVTPPLADLQTNDLVPYRTLTTQAPVAVMVGHMQVPG
                     LTGTDPASLSPAAYGLLRSGDYGGPGFGGLVFSDDLSSMGAINQRYGVADAALRALQA
                     GADVALWVTTDEVPAVLDRL"
BASE COUNT          226 a          543 c          539 g          224 t
ORIGIN      
        1 aagaggtagc cgtaggcctg gttctgtgcc tggccgaacg gcagttcgct gttccacagg
       61 ttgagcgcgc gggccaggaa ccgcagcggg ttggcggtca ggtcgagctt ggtgtccggc
      121 gagatctttc ccggcgactg cacgaacgtc agggccagcg acaccgccgc aaccaaccac
      181 agccagcgac gagcgagcgg cgctgggggc gccggtgaag cggcggcggt caggctagct
      241 gcggtcgccg tactcgacac ggttcagcac ggccgacgcc ggatcgcctg cctgcaccgg
      301 cggcttggag tcctgctgca ccatcagcgt gatgccgaag atggcggccg cgcccagcag
      361 taggccgaca acgatgcttg ccgcagcggg caccagaaac cggtccatgc ggccaaggta
      421 acacgcggcc cacgtaggca gctgagcgcg ccatgacagc atgaccccat ggccctgccc
      481 cgtgtactcg gtgcgctcgc ggcgctgacc ggcttgttgc tggcctgctc gcccgccacc
      541 gagaagcccg ccgcttcgcc ctctagcggc ccgtcgaaca cgtcgttggg ccggccgctg
      601 cccggtgcgc ccgtaccgcc ggcctgcggt gatgtgctgg cgtcgatgtc gacccgcgac
      661 aagctggctc agctgctgat ggtcggtgtc accggcgccg acgacgcgcg ggccgtcgtg
      721 gacaaccaac acgtcggcgg gatcatgatc ggcagctgga ccgacctgtc gatgatgggg
      781 gcaccgctcg ccgacatcgc cgcgtccgcg ggcccgctgc ctttggcggt gagcaccgac
      841 gaggaaggcg gccgggtgtc ccggcttgcc gacatgatcg gcgaacagcc gtcgccgcgg
      901 gtgctctcgc agaccaaaac acctgccgag gtgcacgaca tcgcgttgca gcgcggccag
      961 gcgatgcggg gcctcggcgt caccatcgat ttcgcaccgg tcgtcgacgt caccacggaa
     1021 tccgatgacg aggtgatcgg cgatcgctcg ttcggttcgg atcccgcgca ggtcgtcgag
     1081 ttcgcgggcg cctatgccca aggcctgcgc gacgccggtg tgctgccggt gctgaagcac
     1141 ttccccggcc acggccacgc gtcgggcgac tcccacgagg gcggggtggt gaccccgccg
     1201 ctggccgacc ttcaaaccaa cgacctggtg ccctaccgca ccctgaccac gcaggcgccg
     1261 gtcgccgtca tggtcggcca catgcaggtg cccggcctga caggcaccga ccccgccagc
     1321 ctgagccccg ctgcgtacgg acttctgcgc tcgggcgact acggcgggcc cgggttcggc
     1381 gggctggtct tcagcgacga cctgtccagc atgggcgcca tcaatcagcg ctacggcgtc
     1441 gccgatgcgg cgctgcgcgc gctgcaggcc ggtgccgacg tcgcgctgtg ggtcaccacc
     1501 gacgaggtgc ccgcggtgct ggaccggctg ga
//
LOCUS       DASYLX010001209         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1312005,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001209 DASYLX010000000
VERSION     DASYLX010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312005"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGP27_28470"
     CDS             <1..202
                     /locus_tag="VGP27_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV7855265.1"
                     /translation="VASDVRTHFGEKVLGTVIPRSVKVSEAPGYGMTILHYDPGSRGA
                     LSYMDASREIAERGAAQRGQVR"
     gene            199..1176
                     /locus_tag="VGP27_28475"
     CDS             199..1176
                     /locus_tag="VGP27_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV7855266.1"
                     /translation="MTQPSRKRSGLGRGLASLIPTGPADGDTATIGPRMGDAAADVVL
                     GGGNGDTDAVGAVYREIDPAVIERNPRQPRQVFDEEALTELVHSIREFGLMQPIVVRS
                     IPGQPGGPRYQLVMGERRWRAAQQAGLATIPAIVRETADDSMLRDALLENIHRAQLNP
                     LEEAAAYQQLLDEFEVTHDELAARIGRSRPLITNMIRLLRLPIAVQRRVAAGVLSAGH
                     ARALLALEGGADVQEELAARIVAEGLSVRATEEAVTLANREDHSTPSAPRRKPIHMPG
                     LQDVAERLSTAFDTRVTVSLGKRKGKIVVEFGSVDDLQRIVDLMNAAKA"
     gene            1394..>1878
                     /locus_tag="VGP27_28480"
     CDS             1394..>1878
                     /locus_tag="VGP27_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV7855267.1"
                     /translation="MSTRITPLRLDAFEQLPKHARRCVFWEVDPSTLGGDDHLSDPEF
                     EKEAWLSMVMLEWGSCGQTAVAARLGPGGGEILPGTDEPCLGYAFYAPPGVVPRARLF
                     PSGPVSADAVLLTSLGVEAHARTGDALSADGVPQSLIVAVVADLVRRGVRALEAFGRT
                     EA"
BASE COUNT          308 a          608 c          670 g          292 t
ORIGIN      
        1 ggtcgcgtcc gacgtcagaa cgcacttcgg cgagaaggtt ctcggcaccg tcatcccgcg
       61 cagcgtcaaa gtgtccgagg cgccgggata cgggatgacg atcctgcact acgacccggg
      121 ctcacgtggc gcgctgagct atatggacgc cagccgcgaa atcgccgagc ggggcgcagc
      181 ccagagagga caggttcgat gacccagccg tcgcgcaaga ggagcggcct cggccgcggc
      241 ctggcttcgt tgatcccgac gggtccggct gatggcgata cggccacgat cgggcctcga
      301 atgggtgacg ccgcggccga cgtggtgctc ggcggaggta atggggacac ggacgcggtc
      361 ggcgccgttt accgcgagat cgacccggcc gtcatcgaac ggaacccgcg gcagccgcgg
      421 caggtgttcg acgaggaggc gctcaccgaa cttgtgcact ccatccgcga gttcgggctg
      481 atgcagccga tcgtggtgcg ctcgattccg ggccagcccg gcggtccgcg ataccagctc
      541 gtcatggggg agcgccggtg gcgcgccgcc cagcaggccg gcctagccac catccctgcc
      601 atcgtgcggg agaccgccga tgacagcatg ctgcgcgacg cgctgttgga gaacatccac
      661 cgcgcacagc tgaatccact ggaggaggcc gccgcctacc aacagctgct cgacgagttc
      721 gaggtcacgc acgacgaact cgcggcgcgc atcggccggt cgcggccgtt gatcaccaac
      781 atgatccggc tgctgcggct gccgatcgcc gtccagcgcc gcgtcgccgc aggcgtgttg
      841 tcggcgggcc acgcacgggc gcttctcgcg ctggagggtg gcgcggacgt acaagaggaa
      901 ctcgccgcgc gcatcgtcgc cgagggcctg tcggtgcgtg cgacggaaga ggcggtgacg
      961 ctggcgaacc gggaagacca ctcgaccccc tccgccccgc gccgcaagcc gatccacatg
     1021 ccgggcctgc aggacgttgc tgaacggctg tcgactgcct tcgatacccg ggtgacggtg
     1081 agcctcggca aacgcaaggg caagatcgtc gtcgagttcg ggtcggtcga cgacctgcaa
     1141 cggatagtcg acctcatgaa cgctgcgaag gcctgaccta ggacacgggg aaattacgtc
     1201 actgtgacac atcgatcacg gcgcgagccg ctgtcggcat gcctcgcagc aatcctcatg
     1261 tggtgcaaca tctttggcag atcgggcaac tcgggatgag cgaaccggat gaaaaccggc
     1321 cttcgggcac gcatcctcct attctggagt ggcggcgcca tgtgtcggcg ccgcccggaa
     1381 ctcggggagc ttagtgtcga cgcgaatcac gcccctgcgg ctcgatgcgt tcgagcagct
     1441 gcctaagcac gcgcgccggt gcgtgttctg ggaggtggat ccgtccaccc ttggcggcga
     1501 tgaccacctg tccgacccgg agttcgagaa ggaagcgtgg ctgtcgatgg tcatgctgga
     1561 gtggggttcg tgcgggcaga cggcagtcgc cgcccgactg gggccggggg gcggcgagat
     1621 cctcccgggc accgacgaac cgtgtctggg gtatgcgttc tacgcgcctc ccggtgtggt
     1681 gcctcgtgca cggctctttc cgtccggacc ggtgagcgcc gacgctgtac tgctcacctc
     1741 tctcggtgtg gaggcgcacg cgaggaccgg tgatgccctg agcgcggacg gagtgccgca
     1801 gagcttgatc gtggcggtcg tcgccgatct ggtgcgacgc ggagtgcggg cactggaagc
     1861 gttcggacgc accgaggc
//
LOCUS       DASYLX010001210         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3839541,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001210 DASYLX010000000
VERSION     DASYLX010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3839541"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VGP27_28485"
     CDS             complement(<1..167)
                     /locus_tag="VGP27_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV7855268.1"
                     /translation="MTRNFLRDDDLSPDEQAEVLALAAQLKQDPFARRPLEGPRGVAV
                     IFDKNSTRTRFS"
     gene            complement(167..1369)
                     /locus_tag="VGP27_28490"
     CDS             complement(167..1369)
                     /locus_tag="VGP27_28490"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEV7855269.1"
                     /translation="MTSVGGERSDQGKSLQERWEHVMMNNYGTPAVSLASGDGAVVTD
                     VDGKSYVDLLGGIAVNVLGHRHPAVIDAVTSQMNTLGHTSNFYATEPGVALAEALVGL
                     LGAAGRVFLCNSGTEANEVAFKITRLTGRTKIVAAQGAFHGRTMGSLALTGQPAKQEP
                     FAPLPGDVVHVPYGDVAALAEAIDDATAAVFLEPIMGEAGVIVPPEGYLVAARELSTT
                     RGALLVMDEVQTGMGRTGTFFAHQHDGITPDIVTLAKGLGGGLPIGACIAVGPAGELL
                     TAGLHGSTFGGNPICAAAALAVLRVLADEDLVSRADVLGKTLHHGIESLRHPLVDHVR
                     GRGLLRGIALSAPEGKAVEAAARDAGFLVNAAAPDLIRLAPPLIISEAQIAEFLTALP
                     GVLDKAGS"
     gene            complement(1366..2268)
                     /gene="argB"
                     /locus_tag="VGP27_28495"
     CDS             complement(1366..2268)
                     /gene="argB"
                     /locus_tag="VGP27_28495"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEV7855270.1"
                     /translation="MTSFDVAGGAAPASTQTKADVLAAALPWLKQLHDKIVVVKYGGN
                     AMTDDALRRAFAADMVFLRNCGIHPVVVHGGGPQISAMLKRLAIAGDFKGGFRVTTPE
                     VLDVARMVLFGQVGRELVNLINMHGPYAVGITGEDAHLFTAVRRSVIVDGVATDIGLV
                     GDVERVNTEAVLDLIGAGRIPVVSTIAPDADGVVHNINADTAAAALAEALSAEKLLML
                     TDVEGLYTSWPDRDSLVSEIDVAALTQLLPTLETGMVPKIEACMRAVAGGVPSAHVID
                     GRVEHCVLVELFTDEGTGTKVVKA"
     gene            complement(2265..3482)
                     /gene="argJ"
                     /locus_tag="VGP27_28500"
     CDS             complement(2265..3482)
                     /gene="argJ"
                     /locus_tag="VGP27_28500"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEV7855271.1"
                     /translation="MTDTSAGTTRLVRTQGVTAPEGFRATGIAAGIKASGALDMALVF
                     NEGPDYSAAGVFTRNQVKAAPVLWTQQVLTTGRLRAVILNSGGANACTGPGGFQDSHA
                     TAEAVASALSDWGTDTGAIEIGVCSTGLIGDRLPMDKVRSGVTEIVQEMAGGLTGGEE
                     AARAIMTTDKLPKQVALHHCDNWTVGGMAKGAGMLAPSLATMLCVITTDAVADAAALD
                     HALRRASALTFDRLDVDGSCSTNDTILVLASGASAITPSQADLDEAMLRVCDDLCAQL
                     QADAEGVTKRVVVTVTGAASESDALTAARVVARDSLVKTALFGSDPNWGRVLAAVGMA
                     PVRLDPERIGVSFNGSPVCVDGVGAPGAREVDLSGVDIEVTIDLGVGNDIASVRTTDL
                     SHAYVEENSAYSS"
     gene            complement(3479..4528)
                     /gene="argC"
                     /locus_tag="VGP27_28505"
     CDS             complement(3479..4528)
                     /gene="argC"
                     /locus_tag="VGP27_28505"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV7855272.1"
                     /translation="MQNHCMTSVAVAGASGYAGGEILRLLLSHPAYFDRRLTIGALTA
                     AASAGTTLGEHHPHLLPLSGRVLETTDAEVLARHDVVFLALPHGHSATLAEQLSTDTV
                     IVDCGADFRLTDPAAWERFYGSAHAGSWPYGLPELPGARDRLRGATRIAVPGCYPTAA
                     LLALMPAVAEDLVEPAVTVVAVSGTSGAGRAAKVDLLGSEVIGSARAYNIGGTHRHTP
                     EIAQGLRAVTDKDVTVSFTPVLIPTSRGILATCTARTAAPLSQIRAAYEKAYETEPFV
                     YLLPEGQLPKTGAVIGSNAAQLAVAVDDDAETFIAIAAIDNLVKGTGGAAVQSMNLAL
                     GWPETEGLSIVGVAP"
BASE COUNT          745 a         1551 c         1542 g          751 t
ORIGIN      
        1 gagaagcggg tgcgtgtgga gttcttgtcg aagatcactg cgacaccgcg gggtccctcg
       61 agcggcctgc gggcgaacgg atcctgcttc agctgcgcgg ccagtgccag cacctcggct
      121 tgctcgtcgg gcgacaggtc gtcgtcacgc aggaaattgc gggtcatcag gacccagcct
      181 tgtccagcac accgggcagg gcagtgagga attcggcgat ctgcgcctcg ctgatgatca
      241 gcggcggagc cagccggatc aggtcgggtg cggcggcatt caccaggaac ccggcgtcgc
      301 gggcggcggc ctcgacggcc ttgccttctg gtgcgctcag tgcgatgccc cgcagcagcc
      361 cacgaccccg aacgtggtcg acgagcggat gacgcagcga ctcgatgccg tggtgcaaag
      421 tcttgccgag cacatcggcg cgggacacca ggtcctcgtc ggcaagcacc cgcaacaccg
      481 ccagcgcagc ggcggcgcag atcggattgc cgccgaacgt gctgccgtgc aacccggcgg
      541 tcaacagctc gccggccggt ccgaccgcga tgcaggcacc aatcggcagt ccaccgccga
      601 gccccttggc gagggtgacg atgtctggtg tgatgccgtc atgctggtgg gcgaaaaacg
      661 ttccggtccg gcccattccg gtctgcacct cgtccatgac gagcagcgcg ccgcgcgttg
      721 tcgacagctc acgggcggcg accaggtaac cctcgggagg gacgatgacg cccgcctctc
      781 ccatgatcgg ttcgaggaag accgcggcgg tcgcatcgtc gatggcctcc gccagcgcag
      841 cgacgtcgcc gtaggggacg tgcaccacat cgccgggcag tggggcgaac ggctcctgct
      901 tggcaggctg tccggtcaag gccagcgacc ccatcgtgcg gccatggaag gcgccttgtg
      961 cggcaacgat tttcgtgcgg ccggtaagcc gggtgatctt gaaggcgact tcattggcct
     1021 cggtcccgga attgcagagg aacactcgcc ccgcggcgcc cagcaggccc accagcgcct
     1081 cggccagcgc gacacccggt tcagtggcat agaagttcga ggtgtggccg agggtgttca
     1141 tctgcgaggt gaccgcatcg atgacggcgg gatgacggtg gcccagcacg ttgaccgcga
     1201 tgccgccgag caggtcgaca tacgacttgc cgtcgacgtc ggtgacaacg gcgccgtccc
     1261 cactggccag cgacacagcc ggggtgccgt agttgttcat catcacgtgt tcccagcgtt
     1321 cttgcagcga cttcccctgg tcgcttcgct ctccccctac cgacgtcacg ctttcaccac
     1381 cttcgtgcca gtcccctcat cggtgaacaa ctcgaccagg acgcaatgct cgacgcgccc
     1441 gtcgatgacg tgtgcgcttg gcacaccgcc cgcaacggct cgcatgcagg cctcgatctt
     1501 cggaaccatt ccggtctcca gcgtcggcag cagctgggtc agtgcggcga catcgatttc
     1561 gctcaccagt gagtcacggt cgggccagct ggtgtagagg ccctcgacat cggtgagcat
     1621 cagcagcttc tcggcgctga gcgcctcggc cagcgcggcc gcggcggtgt ccgcattgat
     1681 gttgtgcacg acgccgtcgg catcgggcgc gatggtggac accaccggaa tgcggccggc
     1741 gccaatgaga tcgagcaccg cctcggtgtt cacccgctcc acgtcaccga ccaggccgat
     1801 gtctgtggcc accccgtcga cgatgacgct gcgccgcacc gccgtgaaga ggtgcgcgtc
     1861 ctcgcctgtg atgccgacgg cgtagggccc gtgcatgttg atcaggttca ccagttcccg
     1921 gccgacctgg ccgaacagca ccatccgcgc cacatcgaga acctcgggtg tggtgacgcg
     1981 gaaaccaccc ttgaaatcgc ctgcgatggc gagtcgtttc agcatcgcgc tgatctgcgg
     2041 tccgccgccg tgcacgacga cgggatggat gccgcagttg cgcaggaaga ccatgtccgc
     2101 agcgaaggcg cgccgcagcg cgtcgtcggt catcgcgttc ccgccgtatt tgaccacgac
     2161 gatcttgtcg tgcagttgct tcagccacgg cagcgccgct gcgagcacat cggccttggt
     2221 ctgtgtcgac gccggagccg ccccgccggc gacatcgaat gaagtcatga gctgtatgcc
     2281 gagttctctt cgacgtatgc atgtgacaga tcggtggtcc ggaccgacgc gatgtcgtta
     2341 ccgacaccga ggtcgatcgt gacctcgatg tcgacgccgg acaggtcgac ttcgcgcgcg
     2401 ccgggcgctc cgactccgtc gacgcagacg ggagagccgt tgaacgacac gccgatccgc
     2461 tcggggtcga gccgtaccgg agccatgccg accgcggcca gcacgcggcc ccagttgggg
     2521 tcggaaccga acaacgcggt cttgaccagg ctgtcgcgcg cgaccacgcg ggcggcggtg
     2581 agcgcgtcgg attcgctggc ggcgccggtg acggtgacga cgacgcgctt ggtgacgcct
     2641 tccgcatcgg cctgcagctg tgcacacagg tcgtcgcaca ccctcagcat ggcctcgtcg
     2701 aggtcggcct ggctcggcgt gatcgcgctc gcccccgacg ccagtaccag tatcgtgtca
     2761 tttgtcgagc agctgccgtc gacgtcgagc cggtcgaaag tcaacgcgct ggccctgcgc
     2821 aacgcgtgat cgagtgcggc ggcgtcggcg accgcgtcag tggtgatgac gcacagcatg
     2881 gtcgccagcg acggggccaa catgcccgcg cccttcgcca tcccgccgac ggtccaattg
     2941 tcgcagtggt gcagggcaac ctgtttgggc agtttgtcgg tggtcatgat ggctctggcg
     3001 gcctcttcac cgccggtcag cccacccgcc atctcctgca cgatctcggt gacgcccgag
     3061 cgcaccttgt ccatgggcag ccgatctccg atcaaacccg ttgagcacac gccgatctcg
     3121 atcgcaccgg tgtcggtgcc ccagtcggac agggccgaag cgacggcctc ggccgtggcg
     3181 tggctgtcct ggaacccgcc cggacccgtg cacgcgttgg ctccaccgga gttgaggatc
     3241 accgctcgca gtcgcccggt ggtcagcacc tgttgggtcc acagcaccgg cgccgccttg
     3301 acctggttgc gggtgaacac gcccgcggcg ctgtagtcgg gcccctcgtt gaacaccagc
     3361 gccatgtcca atgcgccgga agccttgatg cccgcggcga tcccggtggc acggaagccc
     3421 tcgggcgccg tgacgccctg ggtgcgaacc agccttgtcg tacccgcaga tgtatcggtc
     3481 acggcgccac tcccacgatc gaaagtcctt cggtctcagg ccagccgaga gcgaggttca
     3541 tcgactgcac ggccgcgcca ccggtgccct tgaccaggtt gtcgatcgcg gcgatcgcga
     3601 tgaacgtctc cgcatcgtcg tcgacggcga ccgcgagctg tgcggcgttg ctgccgatca
     3661 ccgcaccggt cttgggtaac tgcccctcgg gcagcaggta cacgaaaggc tcggtttcat
     3721 acgccttctc gtatgccgcg cgaatctgcg acaacggcgc agccgtacgg gcggtgcatg
     3781 tcgcgaggat gccgcgcgag gtcggtatca gcaccggcgt gaacgagacc gtcacgtcct
     3841 tgtcggtgac ggcgcgcagc ccctgcgcga tttccggagt gtgccgatgc gtgccgccga
     3901 tgttgtaggc ccgagcggag ccgatgacct cggagccgag caggtcgact tttgcggcgc
     3961 ggccggcacc ggatgtgccg ctgacggcga cgacggtcac ggccggctcg acgaggtcct
     4021 cggcgacagc cggcatcagc gccagcagcg ctgccgtcgg ataacagccg ggaacagcga
     4081 tgcgggtcgc gccgcggagc cggtcgcggg cgccgggcag ttccggcagc ccgtacggcc
     4141 agctacccgc gtgagccgaa ccgtagaacc gctcccacgc cgccgggtcg gtgagccgaa
     4201 aatcggcgcc gcagtcgacg atcacggtat cggtgctcag ctgctcggcg agggtcgcgg
     4261 agtgaccgtg cggcaacgca aggaaaacga cgtcgtggcg ggccaacacc tcggcgtcgg
     4321 tggtctccag cactcgacca ctgagcggca gcagatgcgg gtggtgttcg ccgagcgtgg
     4381 tgcctgcgct ggcggccgca gtcagcgcac cgatcgtcag gcgccggtcg aagtaggctg
     4441 ggtggctgag cagcagccgg aggatctcac cgccggcata tccgctggca ccggcgacgg
     4501 ccacagaagt catgcagtga ttctgcattt ttatgcagaa tgttgcaaat caattaagcg
     4561 ctgatcagcc caatcgcaag tccgggacc
//
LOCUS       DASYLX010001211         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_655812,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001211 DASYLX010000000
VERSION     DASYLX010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655812"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..301)
                     /locus_tag="VGP27_28510"
                     /pseudo
     CDS             complement(1..301)
                     /locus_tag="VGP27_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(310..>1902)
                     /locus_tag="VGP27_28515"
     CDS             complement(310..>1902)
                     /locus_tag="VGP27_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7855273.1"
                     /translation="PSADDNGKPGDDHRRMDGYDYVIVGAGSAGCVLAARLSEDPDAR
                     VLLLEAGGEASGIGAIADPRLWATNLKTDVDWGYDTEVQPGTGRSHAFARGRVIGGSS
                     AINGMIFLRGDRTVYDAWANAGNAGWDFESVLPYFKRSETVRGRDRHLRGSEGPLRPA
                     PVARPNPIARSFIDACLQAGHPSTDDVNGASRDGVALHDLNIVDGMRQSAADAYLTAA
                     VRARPNLVIESRTRVLRLLLKSDRCVGVGVVADGVQRDIRAAQEVILSAGAIDTPRLL
                     MLSGIGPADELTQVGIDVAVDLPAVGRNLQDHTVTGLIYQARRTIPPATANHGEASLL
                     WRSSAAEPACDLHLVICDVPITSMPTPANCCTLLLANLRPASRGLTRLRSADPTQPPL
                     IAPNYLAEQSDLEKVLLGLATAREIAAAPAFGDWGLREVLPGPDTTARDALTAFVRVA
                     TETYNHASGTCRMGPGDGSVVDSTLAVHGVQALRIADASIAPTIPDANTHATVIMIAE
                     KAADLIKVAQSPALARTHERVA"
BASE COUNT          261 a          623 c          705 g          313 t
ORIGIN      
        1 agtcgcggat gacggccttg gtccgcatca ggaaatcggc caggtagccg tcgtgcggga
       61 tgcccggctc catccagtag gtggggtggt cgccgacata gacgttgctg atcgtcggtt
      121 cggcgacgag cgcatccagc agccgcgtgt cgtgcgtgat cgcggtgagc accagcgtct
      181 ctttgagcgg atcgacgccg gccgcgcgcg tccacggggc gacccagacg caggggaagg
      241 acagttcgac gccggcctgt ggcgcgtctg gccggtcgag ctggtgcacg gcgggtcgca
      301 gagttcatct caagcaaccc tctcgtgcgt ccgcgccagt gctgggctct gcgcgacctt
      361 gatgaggtcg gctgccttct cggcgatcat gatcacggtg gcgtgtgtgt tggcgtcggg
      421 gatggtcggc gcgatcgatg cgtcggcgat gcgcagcgcc tgtacgccgt gcaccgcgag
      481 ggtcgagtcg acgacgctgc cgtcgcctgg gcccatgcgg caggtgccgc tggcgtggtt
      541 gtatgtctcg gtcgcgactc gaacgaacgc ggtgagggcg tcgcgagctg tcgtatcggg
      601 gccgggcagc acctcgcgca ggccccagtc gccgaaggcg ggcgccgcgg cgatctctcg
      661 agctgttgcc aggccgagca gtaccttctc gagatcggat tgctctgcga ggtagttcgg
      721 ggcgatgagc ggtggttgtg tcgggtcggc gctgcgcagc ctcgtcaggc cgcggcttgc
      781 gggtcgcagg ttggcgagca gcagggtgca gcagttcgcc ggcgtgggca tcgacgtgat
      841 cgggacgtcg cagatcacga ggtgcagatc gcaggccggc tcggcggccg agctgcgcca
      901 cagcagcgag gcttcgccgt ggttggctgt cgcgggcggg atcgtccgtc gcgcctggta
      961 gatcaggccg gtgacggtgt ggtcctgcag gttccgaccg acggccggga ggtctacggc
     1021 tacgtcgatc ccgacctgtg tcagctcgtc tgcgggaccg atccccgaga gcatgagcag
     1081 ccgaggcgta tcgatggcgc ctgcgctgag gatgacctcc tgcgccgcgc ggatgtctcg
     1141 ctgcacgccg tctgccacca cgcccacgcc gacgcaccga tcggatttca gcagcagccg
     1201 aaggacgcgg gtccgcgact cgatcacgag gttcgggcgc gcgcggaccg cggcggtcag
     1261 gtacgcgtcg gctgcgctct ggcgcatccc atcgacgatg ttcaggtcgt gcagggcgac
     1321 gccgtcgcgg ctggcgccgt tgacgtcatc ggtgctgggg tgcccggctt ggaggcacgc
     1381 gtcgatgaac gagcgggcga tcggattggg ccgcgccacg ggagcgggtc gcagcggccc
     1441 ctcgctgccg cgcaaatggc gatcgcggcc ccggacggtc tcggagcgct tgaagtaggg
     1501 caggacggac tcgaagtccc agccggcatt cccggcgttc gcccacgcgt cgtacacagt
     1561 ccggtcgccg cgcaggaaga tcatcccgtt gatggcgctg gaaccaccga tgaccctccc
     1621 gcgcgcgaac gcgtggctgc gacccgttcc gggctgcacc tccgtgtcat agccccagtc
     1681 gacgtccgtc ttgaggttcg tcgcccacag gcggggatcg gcgatcgcgc cgatgcccga
     1741 cgcctccccg ccggcctcca gcagcagcac gcgagcgtca gggtcctccg acagacgcgc
     1801 cgcgagcaca cacccggccg acccggcacc gacgatcacg tagtcgtagc cgtccatgcg
     1861 ccgatggtca tcgccgggct tgccgttgtc gtcggcgctc gg
//
LOCUS       DASYLX010001212         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_546396,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001212 DASYLX010000000
VERSION     DASYLX010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546396"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VGP27_28520"
     CDS             <1..1107
                     /locus_tag="VGP27_28520"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7855274.1"
                     /translation="SAVRAEVGFYKSTRAVTDDDLLDSCTENLRFALKSLEDGVAFDT
                     SPAVATGCRRAAAGIPLPAVMDAYRVASYRLWDAVVDVATEKRGFSRDMVIQATRRIW
                     RFQNLYTDAMASAYRQQNMHQVLDDEAERAALTEALLDGRTITDYSVWEVAQLLRLPL
                     SGPYVVIAAECPALGKQAFPGISAKLRAVDIFSAWRLLPDIQVGLAHIPSSSTRDTMF
                     KLIERQTVKRVGVSPQFDELTDTAQALRYARVAMNARSSNNGGVTVFDNSVLAVAAVS
                     APEVTKKVAAVILEQFDNLTSEEKDVLFDTFRAWVANKGSVNNAAAHLHVHPNTVRHR
                     LHRIERYAGRSLAVPDELAEICLAFEVRENMPAP"
     gene            complement(1210..1605)
                     /locus_tag="VGP27_28525"
     CDS             complement(1210..1605)
                     /locus_tag="VGP27_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEV7855275.1"
                     /translation="MPIYTCTIAQSTLSADTKRELAGEIARIHSSVNHVPSTYVNVAF
                     HELSADGLYTDGEPARPVLVSGWVRAGHPDALTTRLTTEIATAITQITAIPAERVLVV
                     IQSSPARFAMEGGRVLPEPGHEQTWLAGS"
     gene            complement(1955..3568)
                     /gene="mdlC"
                     /locus_tag="VGP27_28530"
     CDS             complement(1955..3568)
                     /gene="mdlC"
                     /locus_tag="VGP27_28530"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HEV7855276.1"
                     /translation="MADQKTVRDVTYDLLRSLGLTTVFGNPGSTEETFLKSFPDDFTY
                     ILGLQEASVLAMADGFAQSTGKPALVNVHTAAGTGNAMGSLIAAYRGNTPLIVTAGQQ
                     TREMVVCEPYLTNSQETLLPQPWVKWAYQPARAQDVPAAFMRAYAIALQPPAGPVYLS
                     IPLDDWERPALGPAVVRTVSHRVSPDTERLRSFANRINRAQRPVLVYGPEVDRSGAWD
                     AGVALAEKLGAPVYGSPLPDRASFPEDHRLYQGPLPMTIAGAEKVLRGHDLVIVIGAQ
                     VFRYYPYVAGEYLPEGTDLLQITNDPALAGAAPVGDSVLGDSLLAMEKLVGMVDDHSD
                     RKVPAAAVHNPEVDWSASPPLAPDAVWSTLASVKPAQSPLVTESTSTMAQQLRWMPTT
                     RPASFFATASGGIGWGLPAAVGLALGDRARGVKRTVVATIGDGSCQYSVQAIYTAAQH
                     RLPVVFVVMRNQEYSILKSFAMLEETPGVPGLDLPGLDIASLASGFGCRTVNVDNCEK
                     LAREFTNALDVDGPTVIVVPTKPQTAYLG"
     gene            complement(3822..4103)
                     /locus_tag="VGP27_28535"
     CDS             complement(3822..4103)
                     /locus_tag="VGP27_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7855277.1"
                     /translation="MGPSTGLFDSGGQRLATPPIRGRAGALKVIGALVTALVAQGRGG
                     VLVIEGPPGIGKSRLLTEVTALADKGAVRTLFGEAFECLFRSHLRRHLW"
BASE COUNT          754 a         1314 c         1310 g          770 t
ORIGIN      
        1 tccgcggtga gagccgaagt cggcttctac aaaagcaccc gtgcggtgac cgacgacgac
       61 ttgctggaca gttgcaccga gaacctccgg ttcgcgttga aaagtctcga agacggcgtg
      121 gctttcgaca cgtcccccgc agtggccaca ggttgcagac gggccgcggc cggtatccca
      181 ctgccggcag tgatggatgc ctaccgcgtg gcctcatacc gtctctggga cgccgtcgtg
      241 gacgtcgcca cagagaaacg cggtttcagc agggacatgg tgatccaggc gacgaggcgg
      301 atctggcgct ttcagaacct ctacaccgac gcgatggcga gtgcctatcg acagcagaac
      361 atgcaccagg tcttggacga cgaggccgaa cgggccgcgc tgaccgaagc actcctggac
      421 gggcgcacca tcaccgacta cagcgtgtgg gaagtcgccc agctgctgcg cttgccgctt
      481 tcggggccgt atgttgtcat tgcggccgag tgtccggctt tgggtaaaca ggcgttccct
      541 ggaatcagcg ccaagctgcg cgccgtcgac atcttctccg cgtggcgcct gctgcccgac
      601 attcaggtcg gcctcgccca cattccatcc agcagcacac gtgacacgat gttcaaactc
      661 atcgaaagac aaaccgtcaa acgggtaggg gtcagcccgc aattcgatga gttgaccgac
      721 accgcgcaag cactccgtta cgcccgcgtc gcaatgaacg cccgcagcag caacaacggc
      781 ggtgtgaccg tgttcgacaa ctctgtgctc gctgttgccg ccgtaagcgc acccgaggtt
      841 acgaagaagg tggcagccgt catcctggag caattcgaca acctgacaag cgaagaaaaa
      901 gatgtactct tcgacacctt cagggcgtgg gttgccaata agggctcggt caacaatgcc
      961 gccgcccatc tgcatgttca cccgaacact gtccggcacc gtctgcaccg catcgagcgg
     1021 tacgccggca ggtcgctcgc ggtgcccgat gaactcgccg aaatatgcct ggcgttcgag
     1081 gtccgcgaaa acatgcctgc accatagggt ctcggtaagc cctagcgccg atatccgggc
     1141 ctacgccgct gggcgttggc gacatatcac cgtgtcggca acggcgtgaa tagggacggt
     1201 cagcttggtt caacttccgg ccaaccaggt ttgttcatga cctggttcag gcagaacacg
     1261 gccgccttcc atggcgaaac gcgctgggct gctctgaatg acgacaagca ctcgttccgc
     1321 cggtatggcg gtgatctggg tgatcgccgt cgcgatctcg gtggttagac gtgtggtcaa
     1381 tgcatccgga tggcccgcac gcacccagcc gctgaccagc accggcctcg ccggttcccc
     1441 gtcggtgtag aggccatccg ccgacagctc gtggaatgcg acgttcacgt acgtgctcgg
     1501 cacatggttg accgatgagt gaatgcgcgc gatctcgcca gccagctcgc gcttggtgtc
     1561 ggcactaagg gtcgattgag cgatggtgca ggtgtagatc ggcatggttg cactcctttg
     1621 ggtccagtcg agagtcgccg ccatccacca tcgcgatctc ttcggacggc gtcgacgtct
     1681 tcggcgacca cgatccatgt ctgtttgtgt gctaaaccac agccgctact gcgtattcca
     1741 cggctcccgc ctccacacga ttacgtcgtt gacgttgtca gcgggcacat cgacgtcggt
     1801 tgattgccag gccacattcg gacgggcagc ctccgaccca cagccctgtc gagatcgcgc
     1861 tagcatctgc ggcgacccgt ggtcggtcct gtggtcatag acggatcgac aacaacatca
     1921 gtcctcgcat ttccctgccg gtgcgaggtc tcactcagcc gaggtaggcg gtttgcggtt
     1981 tggtgggcac tacgatcacg gtgggcccgt cgacgtcgag cgcgttggtg aactcgcgcg
     2041 cgagtttctc gcagttgtct acattgaccg tccggcatcc gaaacccgat gccagggagg
     2101 cgatgtcgag gccgggtagg tctagacccg gaacgcctgg tgtttcctcc agcatggcga
     2161 acgatttgag gatggagtac tcctggttgc gcatgacgac gaacaccacg ggtagccggt
     2221 gttgggccgc ggtgtagatg gcctgtaccg agtactggca tgatccgtcg ccgatggtcg
     2281 ccaccaccgt gcgcttgacg ccgcgggcgc ggtctccgag cgcgaggccg accgctgcag
     2341 gaagacccca gccgattccg ccgctggcgg tggcgaagaa cgacgctggg cgagtggtgg
     2401 gcatccagcg caattgttgg gccatcgtcg aagtggattc ggtgacaagc ggtgactggg
     2461 cgggtttcac cgaagccagg gtcgaccaca ccgcatcggg ggcaagtggg ggggacgccg
     2521 accagtcgac ttcgggatta tgcaccgctg cggcgggcac tttccgatcg ctgtggtcgt
     2581 cgaccatgcc cacgagtttt tccatagcca gcagtgagtc gccgagcaca ctgtcgccga
     2641 ccggtgccgc cccggccagg gccgggtcgt tggtgatttg cagcagatcg gtgccctcgg
     2701 gcagatactc gccggccaca taggggtagt agcggaaaac ctgtgctccg atgacgatca
     2761 caagatcgtg accacgcagg actttttcgg cgcccgcgat ggtcatcggc agcggcccct
     2821 ggtagaggcg gtggtcctcg gggaaggaag cccggtctgg gagtgggctg ccgtacaccg
     2881 gtgcgccgag cttctctgcg agtgccacgc cggcgtccca cgcgccgctg cgatcgactt
     2941 cggggccgta gaccagtacg ggccgctgtg cgcggttgat gcggttggcg aagctgcgta
     3001 ggcgctcggt atcggggctg acccgatggc tgaccgttcg caccaccgcc ggacccaacg
     3061 ccggcctttc ccagtcgtca agcgggatgg acaagtagac cggccctgct ggcggctgaa
     3121 gggcgatcgc gtaggcccgc atgaacgccg cgggaacatc ctgggcgcga gcgggctggt
     3181 aggcccactt cacccacggc tgaggcaaca gtgtctcctg gctgttggtc aaatatggtt
     3241 cacacacgac catttcgcgt gtctgctggc ccgcggtgac gatgaggggg gtgttgccgc
     3301 ggtaggccgc tatcaggctg cccatggcat taccggtgcc cgctgcggtg tgcacattga
     3361 ccagtgccgg cttgccggtc gactgtgcga acccatcggc catcgccagc accgaggcct
     3421 cctgcagccc cagaatgtag gtgaagtcgt cggggaaact cttcagaaac gtctcctcag
     3481 tggagccggg attgccgaaa acggtcgtca gtcccagcga acgaagcaag tcataggtca
     3541 cgtcgcggac ggttttctgg tcggccatcg ctgccaatcc ctttcgtgta attaaacgtt
     3601 gccaccgcgt ggtcttcggc gcgctgctgc tcactcttgt ggacctgtcc ggcaccgtcg
     3661 atgttctcct cgacaacgtg acacgtcggc cgttgtccgc gcgttaaatc cacaacgtgc
     3721 tacgtcaggt tcgaatcgtg acatgcgcgc gatcgagctc ggcgctcacg ccgaatactg
     3781 cattgcagca gccgatatca ctgcgccccg gacagatgga gttaccacag atgtcgccgc
     3841 agatgcgatc tgaaaaggca ctcgaatgcc tcgccgaaca aggtgcgcac cgcgcccttg
     3901 tcggcaagtg ccgtcacctc ggtcagcaac cgactcttcc cgatgcccgg cgggccctcg
     3961 atgaccagca cgcccccacg cccctgcgcc accaaggccg tcaccagcgc gccgatcacc
     4021 ttcagcgcgc ccgcccggcc ccgaatcggc ggagttgcga gccgctggcc gccactatca
     4081 aaaagaccgg tcgaaggccc catggggtgt cctgtctacc gttactggac tgccaatgca
     4141 gtgcgggt
//
LOCUS       DASYLX010001213         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2083680,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001213 DASYLX010000000
VERSION     DASYLX010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083680"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGP27_28540"
     CDS             <1..330
                     /locus_tag="VGP27_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7855278.1"
                     /translation="TTRDVELSGVKMAAGDKVTLWYGSANRDESKFADPWAFDLRRHP
                     NPHLGFGGGGAHFCLGANLARREITVAFEELHRQLPDLTATEEPDRLHSAFIHGIKRL
                     PVAWTPR"
     gene            399..1496
                     /locus_tag="VGP27_28545"
     CDS             399..1496
                     /locus_tag="VGP27_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855279.1"
                     /translation="MQADRFRQLMNAKGPYASVYFDDSHDTEDAETQLDIKLRDLRAL
                     LEEQGAEPAVGDRLEQAVRGSDPPVGKRGRAVVTAANGVVFDQHLIRPTPLPVVRVST
                     LPYLVPVIEHGFDQPPFAVVAVDHAGGDIEIHHDGGVHKESVDGTEYPVHKASGAETS
                     GYGDAQRTADSARIKNIQEVADRVTRLVDEYSPDVLFVVGEVRSRKDFISQLPERVAQ
                     RSVELTVGARTDIAEDQLHQGISEEFERRHCAAIEDVAQRFTAELGRGSGLATEGLAG
                     VTAALRCAAVETLIIGGMGDATVVTGDDLATVSPSADVLSQWGESPSQVFRADEALPM
                     VAISIGASLMPAAEGIAPADGVGAVLRYTVT"
     gene            complement(1497..>2063)
                     /locus_tag="VGP27_28550"
     CDS             complement(1497..>2063)
                     /locus_tag="VGP27_28550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7855280.1"
                     /translation="VESWRIHRSIGMDGARLLETLSGDADDDTQKRAKDLHLRYLREA
                     APLMRRLPGARELLERIRSLDMQIVLATSSSQEELALTRPVLDCEDLLTAATSSKDVD
                     VAKPEPTIIEVALQRAGADAGDAVYVGDAVWDIIACDRAGVRAIGLLSGGVSRGELEN
                     AGAEAVFENPRELADHLDGTCIAALANR"
BASE COUNT          353 a          687 c          687 g          336 t
ORIGIN      
        1 accactcgtg acgtcgagtt gagcggtgtg aagatggctg ccggtgacaa ggtcacgctg
       61 tggtacggct cggccaaccg cgacgagtcg aagttcgccg atccgtgggc cttcgacctg
      121 cgccgccatc cgaacccgca cctggggttc ggcggcggag gcgcgcactt ctgcctcggc
      181 gccaacctcg ctcgccgcga gatcaccgtc gcgttcgagg aactgcaccg acagctgccc
      241 gatctcaccg ccactgaaga gccggaccgg ctgcactccg ccttcatcca cggcatcaag
      301 cggttgcccg tcgcctggac cccgcgctag acggttgacg ccgcgtgttt ggggcatccc
      361 agaccctgca ggcgacgaac accggatacg ggagaaccat gcaagccgat cgtttcaggc
      421 agctgatgaa tgcgaagggg ccgtacgcgt cggtgtactt cgacgattca cacgacaccg
      481 aggacgcgga aactcagctg gacatcaagc tgcgggacct gcgggctctg ctggaggaac
      541 agggtgccga gcccgccgtg ggcgaccggc tcgagcaggc cgtcagaggg tcggatccgc
      601 cggtcggcaa gcgcggccgc gccgtggtga ccgctgcgaa cggcgtggtg ttcgatcaac
      661 acctcatacg cccgacaccg ttgcccgtcg ttcgcgtgtc gacgctgccg tatctcgtac
      721 ccgtcatcga gcacggcttc gaccagcccc cgttcgcggt ggtcgcggtc gaccacgccg
      781 gcggcgacat cgaaatccac cacgacggtg gtgtgcacaa ggagagtgtg gacggcacgg
      841 agtatcccgt gcacaaggcg tccggcgccg agacgtcggg ctacggcgac gcccagcgga
      901 cggcggacag tgcgcgaatc aagaacattc aggaagtggc cgaccgggtc acccggctcg
      961 tcgacgagta ttcacccgac gtcctctttg tcgtcggcga ggtgcggtct cgcaaggact
     1021 tcatcagcca gctgcccgaa cgggtcgcgc aacgatccgt cgagctcacc gtcggcgcgc
     1081 ggaccgacat cgccgaagat caactgcatc aagggatctc ggaagagttc gagcgtcggc
     1141 actgcgcggc gatcgaagac gtcgcgcagc gtttcaccgc cgaactcgga cgggggtccg
     1201 gtctggccac cgagggattg gccggagtga ccgcggccct tcgctgcgcc gcggtcgaga
     1261 ccttgatcat cggtggcatg ggtgatgcca cggtggtcac cggcgacgac ctcgcgacgg
     1321 tgtcaccgtc cgccgacgtg ttgtcgcaat ggggcgagtc accgagtcag gtgttcagag
     1381 ccgacgaagc tctgccaatg gtggccattt cgatcggcgc gtcgctgatg cccgccgcgg
     1441 agggaatcgc gcctgctgac ggtgtgggcg ccgtactgcg ctacaccgtg acgtgatcac
     1501 cgattcgcca acgccgcgat gcaggtgccg tcgaggtggt cggccagttc ccgggggttc
     1561 tcgaaaaccg cttcagcacc ggcgttctcg agttcgccac gagaaactcc tccgctgagg
     1621 agcccgatgg cgcggacgcc cgcgcgatcg caggcgatga tgtcccacac ggcgtcaccg
     1681 acgtagacgg cgtcacccgc atccgccccg gcccgctgca gtgccacctc gatgatggtc
     1741 ggttcgggct tggcgacgtc gacgtccttc gatgaggtcg cagccgtcaa caggtcctcg
     1801 cagtcgagga cagggcgggt cagcgccaat tcctcttgac tggacgaggt ggccagcacg
     1861 atctgcatgt cgagcgagcg gatccgctcc agcagttcac gggcgccggg tagccgtcgc
     1921 atcagcggtg ccgcttccct gagatagcgc agatgcagat ccttggcccg cttctgcgta
     1981 tcgtcgtcgg cgtcacccga cagcgtctcc agcagtcgag ccccgtccat gccgatcgag
     2041 cgatggattc gccacgactc gac
//
LOCUS       DASYLX010001214         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2193451,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001214 DASYLX010000000
VERSION     DASYLX010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193451"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VGP27_28555"
     CDS             complement(<1..837)
                     /locus_tag="VGP27_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7855281.1"
                     /translation="MGNVVKYERTLFEPEHDLFRESYRSFLDRHVAPHHDQWEKDKIV
                     DRSVWLEAGKQGFLGMAVPEEYGGGGNSDFRYNVVLTEETTAGRFSGLGFGLHNDIIA
                     PYLLNLTTEEQKQRWLPKFCSGEYITAIAMTEPGTGSDLQGIKTRAVKVDDHYVLNGS
                     KTFITNGINADLVIVVACTDPDKGAQGFSLLVVERGMEGFERGRHLEKIGLDAQDTAE
                     LSFTDVKVPAENLLGQEGMGFIYLMQNLPQERLNIAVMAAAAMDAVLTETLQYAKERK
                     AFG"
     gene            939..1448
                     /locus_tag="VGP27_28560"
     CDS             939..1448
                     /locus_tag="VGP27_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855282.1"
                     /translation="MSEAQSLQVEDGGTTIAFTFDDMMRYHGPHSPAGVAMAFKVLQR
                     AFGELSPGVDPPRRSVVVRTAFRGPGARDGFEAVTRGVSEGRYTVDRSLLRADRGRLL
                     EDFVFEVAIGHRAATLVLRDGFVTDEFIDLARTENRTAEQERRLAELKAGLAGLVMSA
                     GAADVFDLA"
     gene            1551..1790
                     /locus_tag="VGP27_28565"
     CDS             1551..1790
                     /locus_tag="VGP27_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV7855283.1"
                     /translation="MAENNSGPEEAVKGVVEGVKGKVKEVAGAVTGRDDLYREGQAQQ
                     DKADAQRNAAQKEAEAEGARAAAKTAEKRQEAEQN"
     gene            complement(1842..3257)
                     /gene="hflX"
                     /locus_tag="VGP27_28570"
     CDS             complement(1842..3257)
                     /gene="hflX"
                     /locus_tag="VGP27_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV7855284.1"
                     /translation="MTYPESHINHTPSAGELALDDRTALRRVAGLSTELTDVSEVEYR
                     QLRLERVVLVGVWTEGSAADADASLAELAALAETAGSEVLDGLIQRRDKPDASTYIGS
                     GKAQELRDVVVATGADTVICDGELSPAQLTALEKAVKVKVIDRTALILDIFAQHATSR
                     EGKAQVALAQMEYMLPRLRGWGESMSRQAGGRAGGSGGGVGLRGPGETKIETDRRRIR
                     ERMSKLRHDIKAMKKVRDTQRSRRLGSDMASIAIVGYTNAGKSSLLNALTGAGVLVEN
                     ALFATLEPTTRRGEFGDGRPFVLTDTVGFVRHLPTQLVEAFRSTLEEVVDADLLVHVV
                     DGADAHPLAQINAVRQVINEVLAERDSQPPPELVVINKIDAADGLTLAQLRGAVPGAV
                     FVSAHTGDGLDQLQRRMAQLETPTDTAVDVTIPYDRGDLVARVHADGRVDATEHNPEG
                     TRIKARVPIPLAASLSEFATF"
     gene            complement(3303..4175)
                     /gene="dapF"
                     /locus_tag="VGP27_28575"
     CDS             complement(3303..4175)
                     /gene="dapF"
                     /locus_tag="VGP27_28575"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEV7855285.1"
                     /translation="MMFAKGHGTQNDFVLLPDLDGRLPLHPAAVAALCDRRRGIGADG
                     VLRVTTAGAAADAGVFDRLPEGVAAGDWYMDYRNADGSIAQMCGNGVRVFAHYLRAYG
                     LENRDEFVVGSLAGPRPVVLHGWDATNADVTVEMGKANQLGTGEAIVGGRPFAGLAID
                     VGNPHLACVDPDLAADELAALDVAAPVRFDAEQFPDGVNIEVLTAPSNGSVSMRVHER
                     GVGETRSCGTGTVAAAVAALAYQGATTGTLAVCIPGGEVSVTVTDASSYLRGPSVLVA
                     GGEISEEWWRAQQR"
BASE COUNT          699 a         1410 c         1436 g          726 t
ORIGIN      
        1 gccaaaggcc ttgcgctcct tggcgtattg cagcgtttcg gtcagcacgg cgtccatcgc
       61 cgcggcggcc atcaccgcga tgttcagccg ctcctggggc aggttctgca tcaggtagat
      121 gaagcccatg ccctcctggc ccagcaggtt ctcggcaggg accttgacgt cggtgaacga
      181 cagttcggcg gtgtcctgcg cgtccaggcc gatcttctca agatgcctgc cgcgctcgaa
      241 gccctccatg ccgcgctcga cgaccagaag cgagaagccc tgggcaccct tgtctggatc
      301 ggtgcacgcg acgacgatca ccaggtcggc gttgatgccg ttggtgatga acgtcttgga
      361 cccgttgagg acgtagtggt cgtccacctt gaccgccctg gtcttgatgc cctgcaggtc
      421 gctgccggtg ccaggctccg tcatcgcgat cgcggtgatg tactcgccgc tgcagaactt
      481 gggcagccag cgctgtttct gctcctcggt agtcaggttg agcaggtacg gcgcgatgat
      541 gtcgttgtgc aggccgaagc cgagcccact gaaccgcccc gcggtggtct cctcggtgag
      601 caccacgttg tagcggaagt cggagttgcc gccgccgccg tactcctctg gtacggccat
      661 gccgaggaag ccctgcttgc cggcttccag ccagacgctt cgatcgacga tcttgtcctt
      721 ctcccactgg tcgtggtggg gcgccacatg ccgatcgagg aaggagcggt aggactcgcg
      781 gaacaggtcg tgctcgggct cgaagagagt gcgctcgtac ttgacgacgt tgcccatgta
      841 gacctccgga aatcagtggg ggcttttgcc acgacgatat ctcacccaac cggatggttg
      901 gttgccgggc ggtgcggtgg tggaaataga atcctggagt gagcgaggcg cagtcgttgc
      961 aggtcgaaga cggtggaacg accatcgcat tcacgttcga cgacatgatg cgctatcacg
     1021 gcccgcactc tcccgccggc gtggcgatgg ccttcaaggt cctgcaacgc gcgttcggtg
     1081 agctctcccc cggtgtagac ccgccaaggc gatctgtggt ggtgcgcacc gccttccgcg
     1141 gacccggtgc gcgcgacggg ttcgaggcgg tgacccgggg cgtcagcgag gggcgataca
     1201 cggtcgatcg atcccttcta cgcgctgacc gcggacggtt actcgaggat ttcgtcttcg
     1261 aggtggccat cggccaccgc gcggcgacgc tggtcctgcg cgacgggttc gtcactgacg
     1321 agttcatcga ccttgctcgc accgagaaca ggaccgccga gcaggagcgt cggctcgccg
     1381 aactgaaagc cgggctggca gggctggtga tgagcgccgg cgccgccgac gtattcgacc
     1441 tcgcctgaca ccgccgaccg ttgtgaggtg caccaccaaa cagggatgcg cgtttaacga
     1501 accggccatc cggggaacga aacgggcaac caatgaaaag gagatgcgcg atggctgaga
     1561 acaacagcgg tcccgaagag gccgtcaagg gtgtcgtcga gggcgtgaag ggcaaggtca
     1621 aggaagtcgc gggggccgtc accggtcgcg acgacctcta tcgcgagggt caggcccagc
     1681 aggacaaggc cgacgcgcag cgcaatgccg cccagaaaga ggcagaggcc gagggcgccc
     1741 gcgccgccgc gaagacggcc gagaagcgtc aagaggccga acagaactaa gaaacgcaca
     1801 gcacggaaat ggggcaccga ggggtgcccc atttttgtgg actagaacgt cgcgaactcg
     1861 ctcaggctgg cggccaacgg gatcggcacg cgtgccttga tccgagtgcc ttccgggttg
     1921 tgctcggtgg cgtcgacccg gccgtcggcg tgcacgcgtg ccaccaggtc gccgcggtcg
     1981 tacgggatgg tcacatcgac agcagtgtcg gtcggcgtct ccagttgcgc cattcgccgc
     2041 tgcaactggt cgaggccgtc gcccgtatgt gcggagacga ataccgcgcc gggtaccgcg
     2101 ccccgcaact gggccagtgt gaggccgtcg gcggcgtcga tcttgttgat caccaccaac
     2161 tccggcggcg gttggctgtc acgttcggcg agcacctcgt tgatcacctg acgcaccgcg
     2221 ttgatctgcg ccagcgggtg ggcgtcagca ccatcgacca cgtggaccag cagatcggcg
     2281 tcgacgactt cctccagtgt ggagcggaag gcctcgacca attgggtcgg caggtggcgg
     2341 acgaaaccca ccgtgtcggt cagtacgaac ggtcggccgt cgccgaattc gcctcggcgc
     2401 gttgtgggtt ccaatgttgc gaacaacgcg ttctccacca gtacacccgc tccggtgagc
     2461 gcattgagca ggctcgactt gcccgcgttg gtgtagccga cgattgcgat ggaggccatg
     2521 tcgctgccca gcctgcggct gcgttgtgtg tcgcggacct tcttcatggc cttgatgtcg
     2581 tggcgcagct tggacattcg ttcacggatg cgacggcggt cggtctcgat cttcgtctcg
     2641 ccggggccgc gcagaccgac accgccgccg ctgccgcctg cgcgcccgcc cgcctggcgg
     2701 gacatcgact cgccccaccc acgcagccga ggaagcatgt actccatctg cgcgagtgcc
     2761 acctgcgcct tgccctcccg gctggtggcg tgctgggcga agatgtcgag gatcagcgcg
     2821 gtgcggtcga tcaccttcac cttgaccgcc ttctccagcg cggtcagctg agcggggctc
     2881 agctcgccgt cgcagatcac ggtgtcggcg ccggtcgcga ccaccacgtc gcgcaactcc
     2941 tgcgccttgc cggacccgat gtaggtggag gcgtccggct tgtcacggcg ctggatgagg
     3001 ccgtcgagca cctcggagcc cgccgtctcg gcgagcgcgg ccagttcggc caggctggcg
     3061 tcggcgtcgg cggcactgcc ttcggtccag acgccgacca gcacgacgcg ctccagtcgc
     3121 agctggcggt actcgacctc ggagacgtcg gtgagttcgg tcgacagacc ggccacccgg
     3181 cgcagcgccg tgcggtcgtc gagggccagc tcaccggcgc tcggagtgtg gttgatgtgg
     3241 gattcgggat atgtcatagg cgaataaaga ttcgcacgga gatcactttt catgcacccc
     3301 tattaacgct gctgagcacg ccaccattcc tcggagatct cgccgccggc caccaagacc
     3361 gaaggaccac gcaggtagct gctggcgtcg gtgacggtga cggacacctc gccgccgggg
     3421 atgcagaccg caagcgtgcc ggtcgttgcg ccctggtacg ccagcgcggc gacggcggcg
     3481 gccaccgttc ctgttccgca cgaacgagtt tcgccgacac ctcgctcgtg tacccgcatc
     3541 gacaccgaac cattcgacgg agccgtcagt acttcgatgt tgaccccgtc ggggaactgt
     3601 tcggcgtcga accgcaccgg cgcggccaca tcgagtgcgg ccaactcgtc ggcggccagg
     3661 tcggggtcca cgcacgccag gtgtgggtta ccgacgtcta tcgcgagccc ggcgaagggt
     3721 ctgccgccga cgatcgcctc accggtgccg agctggttgg ccttgcccat ctccacggtg
     3781 acgtcggcat tggtggcgtc ccagccgtgc agcaccaccg gccggggccc cgccaacgag
     3841 ccgacgacga actcgtcgcg gttctccagc ccgtatgcgc gcaggtagtg ggcgaacacc
     3901 cggacgccgt tgccgcacat ctgggcgatc gaaccgtccg cgttgcggta gtccatgtac
     3961 caatcaccag ctgcgacgcc ttctggcagc cgatcgaaca cacccgcgtc cgccgccgcg
     4021 cccgcggtgg tcacccgcag cacaccgtcg gcgccgatgc cgcgacggcg gtcgcacagg
     4081 gcggcgaccg cggcaggatg cagaggcagc ctgccgtcca ggtcgggcag cagcacgaaa
     4141 tcgttctgcg tgccgtgacc ctttgcgaac atcacctgat caggatacgt gtcgccatgc
     4201 ccgcagcgtg ccggtgacgt tgtcgccgcc cgcgccgtcg agccaggtga tccggtgatc
     4261 gcggcggaac c
//
LOCUS       DASYLX010001215         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2741317,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001215 DASYLX010000000
VERSION     DASYLX010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741317"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGP27_28580"
     CDS             <1..235
                     /locus_tag="VGP27_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7855286.1"
                     /translation="NAAKHARASQVEVCARSGDDDLYLSIRDDGVGGADLGKGSGLIG
                     LKDRAEALGGSLTVTSRAGNGTSLDVTIPLDSE"
     gene            complement(878..1144)
                     /locus_tag="VGP27_28585"
                     /pseudo
     CDS             complement(878..1144)
                     /locus_tag="VGP27_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(1318..1632)
                     /locus_tag="VGP27_28590"
                     /pseudo
     CDS             complement(1318..1632)
                     /locus_tag="VGP27_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
     gene            complement(1625..2332)
                     /locus_tag="VGP27_28595"
     CDS             complement(1625..2332)
                     /locus_tag="VGP27_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV7855287.1"
                     /translation="MVTPHATAGASAAGAGNATTVRGPRAGARYAFEAIGTFLLVIAV
                     GAAAESGSPLALLGIGAVLIAMVYAGGHYNPAVTLAVLIRRRVGLSEAIVHWALQIGA
                     GVLAATVVWAIVDPTRAAATAAITLTGHTLVAAFAVELLVIFVLCYMATSGDHRDDSY
                     YSLAIGFTTVASAFAVAAIAGGAFNATLSLGAVVTLSVYLVAQVLGGIAAGVSSLANE
                     SRHEMKRQPHNERNDNA"
     gene            complement(2666..3295)
                     /locus_tag="VGP27_28600"
     CDS             complement(2666..3295)
                     /locus_tag="VGP27_28600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7855288.1"
                     /translation="MSNESDAEFMQADIPVIAPISSHDPIVARSVVGLPGLPTIVAIG
                     PFDDSTHADQLAAAFTVVRRRCQAQLVLLGDGTHRTTVMRKTLALGVRTSVHMEKDLA
                     GDRWADLIAAADVVVPSVAAGPITLLDVLAAGRPVVAPIDPAAVQLIVPASVGLVYRP
                     GDVSGMARALLRLLTSPTLRTGMGCRAQEVAHRYHCKRMALQPAGQAEW"
BASE COUNT          660 a         1091 c         1079 g          602 t
ORIGIN      
        1 caacgccgcc aagcatgccc gcgcgtcgca agtggaagtg tgcgcacgtt ccggggatga
       61 cgacctctat ctctcgattc gcgacgacgg agtcgggggg gccgacttgg gcaaggggtc
      121 aggactgatc gggctcaaag atcgagccga ggccctcggc ggcagtttga cggttacgag
      181 ccgagccggc aatggaacgt cactggacgt cacgatcccg ctcgacagcg agtgaggcat
      241 ccagtcattt ccggcgacca tgccacggcg caaggctgac cgctgcggac tcctagtgac
      301 ccaaatcttc cgtgggatgg cgatccgcat tgcaactagc cgcaatgcgg gattgcggct
      361 ctcctcaagc agttttggcg gctaacgccg acgacaacga tgcccgaatc gagcagtcta
      421 gaggtacatg ccgagccaaa aagcatgtgg aggagatcga tgactatggt cggcgcagac
      481 cgctcgacag gcagccaacg gtgggtgcat ccgacggcag gatccccggc cagagttaca
      541 gcaaacacat cggcggcgcc gcctgctccg tgctcctacc ggttggcgag taagccgccg
      601 gaacccggtt ttacccgaca tcgcgtatag gcgatgtcac ctagatgagg agaaaaatga
      661 agatgaaact gcgatacgtt gcgcccctgg ccctcgccgc gtccgcggca gcgattggac
      721 tggcaccgat cgccgcggcc gacgctaccg tgcaccaaag cccaggaaat gctcaagtcg
      781 ttgctacacc gggtgcagcc tcgcaagagg ccgcccaaaa ccaacagcca tttggcgggg
      841 atatgggcgc cctgatgttc caccaccgct gaccatgtcg tgcgatgtcg gcaccgtcga
      901 cctcgaaccg ctgattgtcg gtgcggtatt tcggcgtcaa gccgagatcg tggaacatgg
      961 cgccgacgta gctcatttcg gggtcgggcc gacgcccgcg ccggcgggat ttgagtgtgc
     1021 cccagaggta tacgcgccga gagtgatgga acagcagttc gtcttccgta tcaagggcca
     1081 gctcggtcgc ggccttcact acaacggtgt cgggaatggt gactccggca attgtttccg
     1141 tcatcgtcgt ttccttgtcg cggcaggggt aagggtgatc tcgatggaga atccagccac
     1201 gaagatcacc aattgcgctg gaagagcccg ggtcattcgt ccacgccttg catgagtgag
     1261 cccgcgcgca cacctttttc gactcgctcg ccgaggtcga tgtcgacgtt gcggaaggtc
     1321 tgggctgccg cgctcaccgg ccacggtgga gaaccggatc accgtgtcgg tggtggtgcc
     1381 gggctgaaac accgccgcct tggtgaacgc gctgacatca ttggtcacct cgaagcggcc
     1441 gaacgcaccc gaacctttgg cgtgcggctg tcgttcgggc acgcgttcgc ggttgaactg
     1501 cgccagctgc tcgatcagat agtgatcatg cagcagaatc ggcccgtccg ggccgatggt
     1561 cagcgaatgc tcatcgcttt ccaccggaat cccggcagta ctcgtcgtcg gaatgggttg
     1621 agggtcaggc attgtcgttt cgctcattgt ggggctggcg cttcatttcg tgtcgcgatt
     1681 catttgcaag ggagctgacg ccggcggcta ttccgccgag cacctgggcg accaggtaca
     1741 ccgacagcgt caccaccgca ccgagcgaca acgtcgcgtt gaacgcgccg cccgcgatag
     1801 cggcgaccgc gaacgcgctt gcgacggtgg tgaatccgat cgccaggctg tagtacgagt
     1861 cgtctcggtg gtccccgctg gtcgccatgt agcacagcac gaagatgacc agcagctcca
     1921 cggcgaatgc ggccaccaga gtgtgaccag tcaacgtgat cgccgccgtc gcggccgcac
     1981 gtgtagggtc gacgatcgcc caaaccaccg ttgccgcgag taccccggca ccgatctgca
     2041 gagcccaatg cacgatggct tcagacaacc cgacccggcg tcgaatcagc acggcaagcg
     2101 tcacagccgg gttgtagtgt ccaccggcat acaccatcgc gatcagcacc gcgccgatgc
     2161 caagaagcgc caacgggctg ccactttccg ccgccgcccc aacggcgatc accagtagga
     2221 atgttccgat cgcctcgaac gcgtaccgcg caccggctcg cgggccgcgc actgtcgtgg
     2281 catttcctgc gccggcggcg cttgcgcccg cggtggcgtg tggcgtgacc atggcttcta
     2341 ccttcgccac aaccgtgggc catgtcgtca gtgctgacga ccaaccacct gtcatgccag
     2401 caccaccacc gggacgcctg ccaaccggta ttggcaaggt ccgcagagtg gtcgacaatc
     2461 ccgccgccaa cggccgctca acttcgagtg gagccccaga aacgggcatt gactcagcca
     2521 tggttacatc aagcagagtc agcgagtggt ggtcgctcga gacgaagaca gcctccgtcg
     2581 aacaggtcga catccggcac ggtgaccaga ttatgatcga ggatttcagc gacccatagg
     2641 atcagtcgtg tagcgatggt acgcattacc attccgcctg tccggccggc tgcagcgcca
     2701 tccgtttgca gtggtatcgg tgcgcgactt cttgtgcacg acagcccatt ccggttcgta
     2761 atgtcggact ggtcaggaga cgtaaaagtg ctcttgccat cccggacaca tcacccggcc
     2821 ggtagacgag tccaacgctg gcgggaacga tcagctgcac agccgcggga tcaatgggcg
     2881 ccactacggg cctgccggcg gccaacacgt ccagcagcgt gatcggtccc gcggcgacgc
     2941 tgggcacgac gacatcggct gcggcaatca ggtctgccca tcggtccccg gcgagatctt
     3001 tttccatgtg cacgcttgtc cgaacaccca gcgccagcgt ctttctcatg acggtggtgc
     3061 gatgcgtgcc gtccccgagg aggacgagtt gcgcctggca gcggcgtcgc accacggtaa
     3121 aggcggcggc cagctgatcg gcgtgggtgc tgtcgtcgaa cgggccgatg gcaacgattg
     3181 tcggcaggcc cggcaggccc actaccgagc gcgcgacgat cgggtcgtgc gaggagatcg
     3241 gtgcgatcac cggaatgtcg gcttgcatga attcggcatc ggactcgttg ctcatgttcc
     3301 gctcacttgt cgtcggggtt cagtgcaagg aagctgagac ctgcagctat cccggccacc
     3361 gtctgggcca gcaggtacac ccataacgtc tgccaggcgg acaaccccat cacagcggcg
     3421 ccgaacgaca cc
//
LOCUS       DASYLX010001216         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3400703,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001216 DASYLX010000000
VERSION     DASYLX010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400703"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGP27_28605"
     CDS             complement(<1..315)
                     /locus_tag="VGP27_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7855289.1"
                     /translation="MSETTQTAPERAEGGVTGAWLAQAAVTEAPDAIVVTDPDGIVRV
                     WNNGAARVFGFSAAEIVGQSLDMIIPEKLRDRHWRGYRETMLTGYTRYGDKLLSVPAT
                     HRD"
     gene            425..1333
                     /locus_tag="VGP27_28610"
     CDS             425..1333
                     /locus_tag="VGP27_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7855290.1"
                     /translation="MLLRQLEYFVAVARERHFARAAEACYVSQPALSTAIAKLERELN
                     VTLINRGQNFEGLTLEGERLVVWAKRLLADHHGLKAEAAALRSGISGTLRLGTGPTVS
                     TTMAAPVAAFCALHPLAKVKVCSRLSSTELLRQLRNFELDAAIAHFGPDDQSGLTVVP
                     LYEEQYVLLVSGEKLVPGARTITWAQAAELPLALLTPDMRFRQFIDKAFASSGALATP
                     QVETDSVASLYAHVATGTWASVVPHTWLRAMPVTSAARAIRLVEPDETAHISMAIHAA
                     APGSVAARAFLNVAAGPHLVGDLSGQ"
     gene            1511..>2571
                     /gene="oxc"
                     /locus_tag="VGP27_28615"
     CDS             1511..>2571
                     /gene="oxc"
                     /locus_tag="VGP27_28615"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEV7855291.1"
                     /translation="MTVAPTRDTEAQGADDTGTLTDGIHLVVDALKLNDVRTIYGVVG
                     IPITDLARLAQASGIRYIGFRHESDAGHAAAAAGFLTKKAGICLTVSAPGFLNGLVAL
                     ANATTNCFPMVQISGSSERHLVDLQRGDYEEMDQLAAARMFAKAAYRVSRAEDIGRGV
                     ARAIRTAVSGRPGGVYLDIPAAVLGEVIDAEQAAGTLWRIVNPAPRQLPEVDMVDSAI
                     DLLADAKRPLIVLGKGAAYAQADEQILEFVETTGVPYLPMSMAKGLLPDDHPQSAATA
                     RSLALKRADVVMLVGARLNWLLGHGDAPQWNPDAKFIQVDIAAAEMDSNQPIAAPLVG
                     DIGSVMEALLERAKPGQITV"
BASE COUNT          438 a          860 c          828 g          445 t
ORIGIN      
        1 gtcgcggtga gtggccggca cgctcaacag cttgtccccg tagcgggtgt atccggtgag
       61 catcgtctct cggtagcccc tccagtgccg gtcccgcagc ttctcgggga tgatcatgtc
      121 gaggctctgg cccacgatct cggctgctga aaatccgaag acgcgcgccg ctccgttgtt
      181 ccacacccga acgattccgt ctggatccgt caccacgatg gcatcgggcg cttccgtcac
      241 ggccgcttgc gcgagccagg ccccggtgac accgccttcg gccctttccg gagctgtttg
      301 cgttgtctcg ctcattgttg ccctcccata tcgccgttcc aaggtggaca tttccgtcaa
      361 cccgcgagta ccataccgaa taagccatac tgtatacaat ctacgatgac gaggaggaca
      421 gcccatgctg ctacgccagc tggagtactt cgtcgccgtg gccagggaac gacacttcgc
      481 ccgtgccgcg gaagcctgtt acgtgtcgca gcctgccctt tctacggcaa tcgccaaact
      541 ggaacgcgag ctgaacgtca cgctgatcaa ccggggccag aacttcgagg gtctgaccct
      601 ggagggcgaa cggctcgtgg tctgggccaa gcgactgctc gctgatcacc acggtctgaa
      661 ggccgaggcg gcggctctcc gttcgggcat ctccggcacg ctccgcctgg gcaccgggcc
      721 cactgtgtcc acgacgatgg cagcgcccgt ggccgcattc tgtgcgttgc acccgctggc
      781 caaggtgaag gtctgctccc gattgtcatc caccgagctg ctgcggcaac ttcgcaactt
      841 cgagctggac gccgccatcg cgcacttcgg gcccgatgac cagtcaggcc tgacggtggt
      901 gccgctgtac gaggagcagt atgtgctgct cgtgtccgga gagaagctgg tgccgggcgc
      961 ccgaaccatc acgtgggctc aggcggcgga gctgccgctg gcgttgctga caccggacat
     1021 gcgatttcgt cagttcatcg acaaggcttt cgccagcagt ggggcgcttg ccaccccgca
     1081 ggtcgagacc gactccgtcg cttcccttta cgcacacgtg gcgaccggaa cgtgggcgag
     1141 cgtggtgccg cacacctggc tgcgcgcgat gccggtgacg agcgccgcga gagccatccg
     1201 gctcgtcgag ccggacgaga cggcccatat ctcaatggcg attcacgcgg cggcccccgg
     1261 ttcggtcgcc gccagagcgt ttctgaacgt ggcggccggg ccgcacctag tcggcgacct
     1321 gagcggccag tgaatgcttc gccgacaacc ggggcggcgc ggaaccccgt gatagccaac
     1381 gtttatcgac tcgttgacaa caagtcttgg acgcgctggc cgaagacgtg aaaagtgtta
     1441 gtaggtaagg gaaatttcga agggcttcgg cttcggcttc ggcttcgaac agttaggaag
     1501 ggtccacatc atgaccgtcg ctcccacgcg agacacggag gcgcagggtg ccgacgacac
     1561 gggcactctc actgatggga tccacctggt cgtcgacgca ctgaaactca acgacgtgcg
     1621 caccatctac ggagtcgtcg ggatcccgat caccgacctc gctcggctcg cacaggcgtc
     1681 gggcatccgc tacatcggct tccgccatga gagcgacgcc ggccatgcgg ccgcggcggc
     1741 agggttcctg acgaagaagg cgggcatctg cctgaccgtg tccgccccgg gcttcctcaa
     1801 cgggcttgtg gcgcttgcca atgccaccac caactgcttc cccatggtgc agatctccgg
     1861 atccagcgag cgccatctcg tggatcttca gcgaggcgac tacgaagaga tggatcagct
     1921 ggcggcggcc aggatgttcg ccaaggctgc ttaccgggta tctcgggccg aggacatcgg
     1981 ccgcggcgtg gcgcgcgcga tccgcaccgc ggtgtccggc cgcccaggag gggtctacct
     2041 cgacatcccg gccgcggtgc tcggcgaggt gatcgatgca gagcaggccg cggggacgct
     2101 gtggcggatc gtcaaccccg ccccgcggca actgccggaa gtagacatgg tcgactccgc
     2161 gatcgacctg ctcgccgacg ccaaacggcc cctgatcgtg cttggcaagg gcgccgcata
     2221 cgcgcaggcg gacgagcaga tcctcgagtt cgtcgagacc accggcgtgc cgtacctgcc
     2281 gatgtcgatg gcgaagggcc tgctgcccga cgatcacccg cagtcggcgg ccaccgcccg
     2341 atcgttggcc ctcaagaggg ctgacgtggt gatgctcgtc ggtgcccggc tgaactggct
     2401 gctcggacac ggcgatgcgc cgcagtggaa cccggacgcc aagttcatcc aggtcgacat
     2461 cgccgccgcg gagatggaca gcaaccagcc catcgcggct ccgctcgtcg gcgacatcgg
     2521 ctctgtgatg gaggcgctcc tcgaacgggc caaacccggt cagatcactg t
//
LOCUS       DASYLX010001217         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_877366,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001217 DASYLX010000000
VERSION     DASYLX010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877366"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGP27_28620"
     CDS             complement(<1..267)
                     /locus_tag="VGP27_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HEV7855292.1"
                     /translation="MVGWLDQLQQRNRVVGFVVAVIYKYVDDQGGYLAALITYYAFVS
                     LFPLLLLLTTALAVVLVGHPDLQGEVLQSALSQLPVIGDQLTQPH"
     gene            complement(468..1019)
                     /locus_tag="VGP27_28625"
     CDS             complement(468..1019)
                     /locus_tag="VGP27_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7855293.1"
                     /translation="MAAPTRTPRTTWIQEGLRALGVGGLDAVRIEALAQALGVSKGGF
                     YWQFGDRNTLLEEMLDTWERVVTDEVINYVENGGGDARTKLRRLFAIAVSTYDMINIE
                     VAIRDWARRDTVVAHRLKRVDDRRMQYMRSLFSVLSDDPDDVEARCLLAFSLFIGNHF
                     IAADHGARSREEVMRSAVEKLLA"
     gene            1343..1606
                     /locus_tag="VGP27_28630"
     CDS             1343..1606
                     /locus_tag="VGP27_28630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855294.1"
                     /translation="MRRDRRAAAAAALVGLVAAALVAASHIVPHWSVISASYLDDVHP
                     DVLSWAVMLLEIASGLFLAVVGIRRIHMPEQVAATTESAEGTP"
     gene            1905..2105
                     /locus_tag="VGP27_28635"
     CDS             1905..2105
                     /locus_tag="VGP27_28635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7855295.1"
                     /translation="MSPFAHGHIVGRNTGETASFTTYLFYTRPAVARTIWTLVSPLHR
                     AVAPYLLQRGNDESALDGIGSP"
     gene            2426..2680
                     /locus_tag="VGP27_28640"
     CDS             2426..2680
                     /locus_tag="VGP27_28640"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7855296.1"
                     /translation="MLASVRKTNRLVTAHEAVISGGFGAEIAARVADAAIWHLGAPQT
                     GGTAVSQRRSPSALVCGQPTWRERPPTHIRRTPRRRVTMK"
     gene            complement(2756..3526)
                     /locus_tag="VGP27_28645"
     CDS             complement(2756..3526)
                     /locus_tag="VGP27_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7855297.1"
                     /translation="MSTARTAVITGATSDRGIGMTVAQRYAREGWAVVILDLDGEKSA
                     KVAADIGNQFAVPAFGHALDVTSEPSVTAARDAVAVEVSTGNLPPVGALANIAGITSP
                     VPFLETTLDLWNTVMAVNATGTYLVTKAFLPDMIEQGWGRIVNMSSVSAQRGGGVFGK
                     VPYSSAKAAILGFTKALAREIADTGVTVNAVTPGAVDTNIRVGTTPEKEAKLAADIPI
                     GRVASTEEIAAVITFLSSQDAGYLTGTTVDVNGGSHIH"
     gene            complement(3535..4362)
                     /locus_tag="VGP27_28650"
     CDS             complement(3535..4362)
                     /locus_tag="VGP27_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV7855298.1"
                     /translation="MPHFDSRLGCSTISFRHQPLGEALATIAASGFAEIDLGALPGTC
                     DHVPYVLDMAAVENVARTVIASGLKVRSINCDIGDLNVPLADGPRAGREAHLDMLVAL
                     AVAIDARALVLPCGAIDQSPVETLETDVARIADELASAADVAASRGVHLWTESLHLHR
                     LCWNLERSQLLADHLPANVGIVMDFSHVVASGGDPVEFVARFGPRIEHVHIRDAAPGN
                     INLSVGRGTVDFERGLKALADAGFAGHFSLELETRDVTHDERPAAAAAAGHLISELI"
     gene            4558..>4703
                     /locus_tag="VGP27_28655"
     CDS             4558..>4703
                     /locus_tag="VGP27_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7855299.1"
                     /translation="MSDEALRMRGPVHGTKDAKRVAIGSGVGAVIETYDFIGFGTAAA
                     LYFGT"
BASE COUNT          845 a         1433 c         1509 g          916 t
ORIGIN      
        1 atgcggttgc gtcaactgat caccgatgac cggaagttgg ctgagcgcag attgcaagac
       61 ttcgccttgc agatcggggt ggccaacaag cacgacggcc agcgcggtgg tcagcaacag
      121 cagcagcgga aacagcgaca caaacgcgta gtaggtgatc agcgcagcca gatagccgcc
      181 ttggtcatcg acgtacttgt agatcaccgc aacgacaaag cccaccacac gattacgttg
      241 ctgcaactga tcgagccagc ccaccatctc ggtcactcct cggagagcgc gtgagctggg
      301 aataccctac gttgaccctg gtcaatcgcg tcggacggcc ccagcgactg cgaccgaagc
      361 ccactgggca atgtgggccg gctcgcacta ccgctttagc gcgccggaga aggccgacgg
      421 ttcagatatc gcctccggcg gccggcacgg ccataactcg gggtttccta ggccaagagc
      481 ttttccactg ccgacctcat cacttcttcg cggcttctcg caccgtgatc ggcagcgatg
      541 aagtggttgc ctatgaagag cgaaaaggcc agcaggcagc gggcttccac gtcgtctggg
      601 tcatccgata ggacactgaa gagcgagcgc atgtattgca tgcgacggtc gtcaacgcgc
      661 ttgagtcggt gggcgacgac cgtgtcgcgt cgcgcccagt cgcggatcgc gacttcaatg
      721 ttgatcatgt cgtaggtgga gaccgcgatc gcgaacagtc gccgcagttt cgttcgcgcg
      781 tcaccaccgc cgttttccac gtagttgatc acttcgtccg tgacgacgcg ctcccaggtg
      841 tcgagcattt cctccagcaa tgtgttgcgg tcgccgaatt gccagtagaa gccgccctta
      901 ctgacaccaa gcgcctgagc cagcgcttct atgcgcaccg cgtccaggcc gccgacgcca
      961 agtgcgcgaa gtccctcttg gatccaagtc gtgcgcggtg tgcgagtcgg ggcggccatc
     1021 gcgcccacct ccctttcagg aataacgcag ccgctcttgt tgaccgaccg ggcaaccata
     1081 cgctaccgta caatacggca ccgtatggat cgtccgtaac ggttgtcacg gatggttgcc
     1141 atggatgagt caccacggat tcggtttgac gggctgggca tagcggccgc catatttttg
     1201 gcggcgaata ttcttcacac ggtcgatcac gtcagacagc acatggcagg actcaatgtc
     1261 gtgatcatga ttggcggtgg cacgttgacc gcggccgcgg tgttagtaac cgtcgttgcc
     1321 ttgcggcgcg accgtcgttg ccttgcggcg cgaccgacgt gcagccgctg ccgccgcgct
     1381 tgtgggactc gtcgctgctg cgctcgtggc cgccagtcat atcgtgcccc actggagcgt
     1441 gatcagcgcg tcatatctcg acgatgtaca tcccgatgtt ttgtcctggg cggtcatgtt
     1501 gctggaaatc gcctccggct tgttcctggc agtcgtcgga atccgtcgca tccacatgcc
     1561 cgagcaggtt gcggcgacaa cggaatccgc cgaagggaca ccatgacatg acaattgacc
     1621 gtccgcgtgc gcgccgatgc gcggtgcctg ccgcggagcc gttaattggg gggtctgact
     1681 ttgactacgc ggactccttt gaaattcgtc tacgacccac agattcacgc tcgcctgaac
     1741 aggcgttccg gaacgctctc gaccgagcgc cagtcatacg gatgctcgta cccccggtgc
     1801 accgctacgt gctcggaatg cgactcggtc cgctttcctc gtctgaacac ctattggggt
     1861 ggaaaatagt cgtgtctacg gccgaggtcg tccaccttga ggccgtgtca ccctttgccc
     1921 acgggcatat cgtgggccga aacaccggtg agacagcgtc tttcacgacc tatctgttct
     1981 acacgcggcc cgccgtcgca cgcacgattt ggaccctggt ctcacctctg caccgcgcgg
     2041 tcgcgcccta cctgctgcag cgcggcaatg acgagagcgc ccttgacggc attgggtcac
     2101 cgtgaagccg gctaacggat aggtctgccc gcaatctcgg tcgtcacctc agcgtggtgc
     2161 ccttggtcga aaagtgatgg gtcctttcca tgtgtcactt tcgttctcgg gcgaattagt
     2221 ttgtcggcct ataggttcgc taccgaacga ggtcatgtat ttgcacttcg tgggcgtgtg
     2281 tttcgaccaa acgatgaacc aggctggcaa gctccatttc atgaacggcg ggcgtgcgcc
     2341 gatgtcgctt gttcatcgag caccgactca cttacggtaa gaagggcccg acgccgccgc
     2401 ccggacttcg tttcgggagg ctgtcatcct cgcttcggtt cgcaagacca atcggcttgt
     2461 cacagcgcac gaggcggtca tctccggtgg gttcggtgcc gaaatcgctg ccagagtggc
     2521 cgatgcggcg atctggcact tgggtgcccc tcaaacgggt ggcactgccg tgagccaacg
     2581 acgtagtcca tctgcgctcg tgtgcggtca acccacctgg cgggaaaggc cgccgacgca
     2641 tatccgcagg acgccgaggc gtcgcgtaac gatgaaatga acagtcgccg ctggtgtcac
     2701 ccatgacccg actcatctgt caccgatgtc gtgaaaccag acccttggct ccgcgtcagt
     2761 gaatgtggct gccaccgttg acgtcaacgg tggtcccggt gaggtagccg gcgtcttggc
     2821 tggacaggaa cgtgatgacg gcggcgattt cttcggtaga ggcgacgcgg ccgatcggga
     2881 tgtcggcggc cagcttggct tccttctccg gcgtagtgcc gacccggatg ttggtgtcga
     2941 ccgcgcccgg tgtcacggcg ttgaccgtca ctcccgtgtc ggcgatctcg cgggcaaggg
     3001 ccttggtgaa gccgaggatg gcggccttgg cggacgagta ggggaccttg ccgaagaccc
     3061 cgccaccgcg ctgcgcggac accgatgaca tgttgacgat gcgaccccaa ccctgctcga
     3121 tcatgtcagg aaggaacgcc ttggtgacta ggtacgtgcc cgtcgcgttg actgccatca
     3181 ccgtgttcca gagatcaaga gtggtttcga ggaacggcac cggtgaggtg atgcccgcga
     3241 tgttggccag ggcgcccacc ggaggcaggt ttccagtgga gacctctacg gcgacggcgt
     3301 ctcgcgccgc tgtcaccgag ggctccgagg tgacatccag ggcgtgtccg aacgccggca
     3361 cggcgaactg gttgccgatg tcggcggcga ccttcgccga cttctcgccg tcgaggtcga
     3421 ggatgacgac cgcccagcct tcgcgggcat agcgctgggc aacggtcatg ccgatgccgc
     3481 gatcggaggt ggcgccggtg ataacggcag tgcgtgcggt gctcatgttt ctccctagat
     3541 gagttcggaa atcaggtgac cggcagcggc cgcggccgcc ggccgttcgt cgtgggtgac
     3601 gtcgcgcgtt tcgagttcga gcgagaaatg gccggcgaaa ccggcgtccg cgagggcttt
     3661 cagtcctctc tcgaagtcga ccgtgccgcg gccgaccgag aggttgatgt tgcccggcgc
     3721 tgcgtcgcgg atgtggacgt gctcaatacg cggtccgaag cgagcgacga actcgaccgg
     3781 atctccgccg gatgcgacga catggctgaa gtccatgacg attccgacgt tggcaggcag
     3841 gtggtcggcg agcagctgcg atcgctcgag attccagcag aggcgatgca ggtgtagcga
     3901 ctcggtccac agatgcacgc cgcgcgaggc ggcgacgtcg gccgccgatg cgagttcatc
     3961 ggcgattcgc gcgacatcag tttcgagcgt ctcgaccgga gactggtcga tcgccccgca
     4021 gggcagcacg agcgcgcggg cgtcaattgc gacggcgagc gcgacgagca tgtcgaggtg
     4081 ggcctcgcgg ccggcccggg gtccgtcggc gagcggaacg ttgaggtcac cgatgtcgca
     4141 gttgatcgac cggaccttca ggccggacgc gatgacggtg cgtgcgacgt tctcgacggc
     4201 ggccatgtcg aggacatacg ggacgtggtc gcacgtacct ggcagtgcgc cgaggtcgat
     4261 ttcggcgaag cccgaagcag cgatggtcgc caacgcctca cccagcggct ggtgcctgaa
     4321 gctgattgtt gagcagccca gtcggctgtc gaaatgcggc atctgctgct cctcattggg
     4381 tcgtgtgata gcgaccacat tagcctattc aactgttaac agtcaacggg caactgtgat
     4441 tcgtgtcgac tgttgacatt gactgtgcgc ggagtcacac tgggtgacgc aacgcactgt
     4501 tgaccgtcga cagtgacgtg attgcccagc cagatcagga ttgaaaggat gaccatcatg
     4561 agcgatgagg ctctgaggat gaggggcccg gtgcacggca ccaaggacgc caagcgggtt
     4621 gcaatcggtt ctggcgtcgg agctgtgatc gaaacctacg acttcatcgg gttcggcacc
     4681 gccgccgcgc tctacttcgg tac
//
LOCUS       DASYLX010001218         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_547299,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001218 DASYLX010000000
VERSION     DASYLX010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547299"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGP27_28660"
     CDS             complement(<1..199)
                     /locus_tag="VGP27_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEV7855300.1"
                     /translation="MALEILPDPKALRSGRRERVLAQMAADDIDILVLGRQANVRYVT
                     GAPQLWVAGTRPFGPICEVIRS"
     gene            complement(199..1440)
                     /locus_tag="VGP27_28665"
     CDS             complement(199..1440)
                     /locus_tag="VGP27_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV7855301.1"
                     /translation="MTTFATAASTAIEIPDLPDMARMYRECGARLRDSMKNKGVDALV
                     LLGNGNVVYATGASWRLLDAGLSHVERPVAIVLADDEHPHLFMPLREGSVSSCEVPAD
                     HIHGPLYLEFDEGVQRFGTVLADLVPAGATVAVDELTGAMRSAQSRLFPSGPPSDAAQ
                     VVGPAKLVKTVDQISCVRNACRITEAAMVDVQKALAPGVRQVDLSAAFVRRAFELGAT
                     ANMLEAIWQVMPTTKTSGAVWTTTGDLALPLLTTERELASGDVLWTDVSITYNGYCSD
                     FGRTWLVGDEVSDRQQAQFEKWREIIDSVLAVTKAGASSGDLARAAIAANGGKKPWLP
                     HFYLGHGIGTNAAEMPMIGTDLGEEFDDNFVFPAGMLLVLEPVIWEDGTGGYRSEEIV
                     VITEDGTTRITDYPYAPYGRA"
     gene            complement(1437..>2485)
                     /locus_tag="VGP27_28670"
     CDS             complement(1437..>2485)
                     /locus_tag="VGP27_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7855302.1"
                     /translation="NHISVADDIFYERFPEELKGAAPRIWYEDGAYMVGMKGKAWTGG
                     DFGRVLMQYDDLAGAASNNIEARIRELKEDGIDKELAFPNAVLALFHYPDKSLRERVF
                     RIYNEHIADLQERSNGHFYGVGLINWWDPKGTRSTLEELKTLGLKTFLLPLNPGKDDD
                     GNIYDYGSTNMDAVWDEIEAAGLPVSHHIGETPPKTPCQNNSVVVGMMVNVDSFREQF
                     AKYVFSGILDRHTTLKIGWFEGGIAWVPTALQDAEHMLASYRHMFNHQLSHDVRHYWN
                     DHMSASFMVDPLGLELVDKIGVENVMWSSDYPHNESTFGYSEKSLKTVVDAVGPEAAT
                     KIVSTNIKNFLGVE"
BASE COUNT          401 a          807 c          813 g          464 t
ORIGIN      
        1 tcgagcggat cacctcgcag atcgggccga acggcctggt gcccgcgacc cagagctgcg
       61 gcgcaccggt cacgtaccgg acgttggcct ggcggccgag caccaggatg tcgatgtcgt
      121 cggcggccat ctgggccagc acccgctcgc gacggccgct gcgcaaagcc tttgggtcag
      181 ggaggatctc cagtgccatc atgcccgccc gtacggggcg taggggtagt cggtgatgcg
      241 ggtggtgccg tcttcggtga tgaccacgat ctcctcgctt cgatagccgc cggtgccgtc
      301 ctcccagatc accggttcga gcaccagcag cattccggcg gggaagacga agttgtcgtc
      361 gaactcctca ccgagatcag ttccgatcat cggcatctcg gccgcattgg tgccgatgcc
      421 gtggccaagg tagaagtgcg gcagccacgg cttcttgccg ccgttggcgg cgatcgcggc
      481 gcgggcgagg tcaccggatg aagcgccggc cttggtgact gccaggacgg aatcgatgat
      541 ctcgcgccac ttctcgaatt gcgcttgctg gcggtccgac acctcgtcgc cgaccagcca
      601 ggtgcggccg aagtcggaac agtagccgtt gtaggtgatg ctgacgtcgg tccacagcac
      661 gtcaccgctc gccagctcac gctccgtcgt cagcaggggc agagccaggt cgccggtggt
      721 ggtccagacc gcaccgctgg ttttcgtggt gggcatcacc tgccagatcg cctcgagcat
      781 gttggcggtc gcccccagtt cgaacgcgcg ccggacgaat gccgccgaca ggtccacctg
      841 tcgcacgccg ggagccagcg ccttctgcac gtccaccatg gcggcttcgg tgatgcggca
      901 ggcattgcgg acacacgaga tctggtcgac cgtcttgacc agcttcgccg ggcccacaac
      961 ctgcgcggca tccgacggcg gccccgacgg gaacagccgc gactgggcgc tgcgcatcgc
     1021 gcccgtcagt tcgtcgaccg cgaccgtggc accggcgggc accaggtcgg ccagcacggt
     1081 gccgaaacgc tggacgccct cgtcgaattc gaggtacagc ggcccgtgga tgtggtcggc
     1141 gggcacctcg cagctcgaca ccgagccctc acgcaacggc atgaacagat gcgggtgttc
     1201 gtcgtcggcc agcacgatcg ccaccggacg ctcgacatgc gaaagcccgg cgtccagcag
     1261 tcgccaactg gcaccggtgg cgtacaccac gttgccgttg cccaacagga cgagtgcgtc
     1321 gacgcccttg ttcttcatgg agtcacgcag cctggcgccg cattcgcggt acatgcgcgc
     1381 catgtccggc aagtctggaa tctcgatggc ggtgctggcc gccgtcgcga acgtggtcat
     1441 tcgacgccca ggaagttctt gatgttggtg ctgacgatct tcgtcgcggc ctcgggcccg
     1501 acggcgtcga cgaccgtctt cagcgacttc tccgaatacc cgaacgtcga ttcgttgtgc
     1561 ggatagtcgg acgaccacat cacgttctcc acaccgatct tgtcgacgag ctccaggccg
     1621 agcggatcga ccatgaacga ggcgctcatg tggtcgttcc aatagtgccg gacgtcgtgg
     1681 ctcagttgat ggttgaacat gtgccggtag gaggccagca tgtgctcggc atcctgcagg
     1741 gccgttggca cccaagcgat tccaccctcg aaccagccga tcttcagcgt ggtgtgccgg
     1801 tcgaggatgc cggagaagac gtacttggcg aactgctcgc ggaacgagtc gacgttgacc
     1861 atcatgccga ccacgacgct gttgttctgg catggcgtct tgggcggggt ctcgccgatg
     1921 tggtggctga ccggcagccc ggccgcctcg atctcgtccc agaccgcgtc catgttggtg
     1981 ctgccgtagt cgtagatgtt gccgtcgtcg tccttgcccg ggttcagcgg gagcaggaac
     2041 gtcttgagtc ccaatgtctt cagttcctcc agcgtgctgc gggtgccctt ggggtcccac
     2101 cagttgatga gcccaacgcc gtagaagtgt ccgttggagc gctcttgcaa gtcggcgatg
     2161 tgttcgttgt agatccggaa cacgcgctcg cgcagcgact tgtccggata gtggaacagc
     2221 gccagcaccg cgttcgggaa ggccaactcc ttgtcgatac cgtcctcttt gagctcgcgg
     2281 attcgcgcct cgatgttgtt cgaggcggca ccggcgaggt cgtcgtactg catgagcaca
     2341 cggccgaagt cgccaccggt ccaggccttg cccttcatgc cgaccatgta cgcgccgtcc
     2401 tcgtaccaga tgcgcggcgc cgcgcccttg agttcctcgg gaaagcgttc gtagaagatg
     2461 tcgtcggcga ccgagatgtg gttgt
//
LOCUS       DASYLX010001219         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_1535332,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001219 DASYLX010000000
VERSION     DASYLX010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535332"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..>1790
                     /locus_tag="VGP27_28675"
     CDS             215..>1790
                     /locus_tag="VGP27_28675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV7855303.1"
                     /translation="MRFHVFSHRGAFVALLICTAVLYLWNLSASGWANSFYSAAAQAG
                     ASNWTAMLFGSSDAANAITVDKTPASLWIMDISVRIFGLHPWSVLAPQALEGVAAVAV
                     LYAAVRRVSGPGAGLLAGAIMALTPVAALMFRFNNPDALLVLMLVVAAYCTQRGCEKD
                     SSRWWLVAAGAAIGFGFLAKMLQALLVLPGFTAAYALAGSRPLGRRLLDVLAMLGAMA
                     VSGGWYLLLVEVWPPDTRPYIGGSQHNSIVELALGYNGLGRLTGDETGGLGNMNFDVG
                     WDRLLGHSMGTEIAWLLPAAVICLGAILVLTRRTARTDQTRAAAIIWGGWLLVTAVVF
                     SFMSGIVHPYYTVALAPAIAACIGVGATLLWQNRFALPAATALAGVAVVTSVLACVLM
                     ARTDGWLPWLRAAVAVGGLASAVLLLVSGRLRTGMARSVAGLAIVVALAAPAAYCVAT
                     AATSHGGAMPSAGPSGGGGMPPGAGGLLSAPTPGPALSGVLAANADDFTWAAAAMGSN
                     NAAGYQLGSGAPVMAIG"
BASE COUNT          237 a          602 c          632 g          319 t
ORIGIN      
        1 gcgatcgtcg ccgcggtggt cagggcgcac aacggcgcca tcaaggtccg cagtgtgccg
       61 ggccggaccg agttcgtggt gaaactgccg gtcaattcac agccaagcca cagcgccggc
      121 aaaaccgacg cttagctgag ccgacgatgc ttggccggtg accttgcttc ttgaggccga
      181 gcagacgcaa gctcgcacaa atcctggctt gggcgtgcga tttcacgtct tctcgcaccg
      241 cggtgcgttc gtcgcgctgc tcatctgcac tgccgtgctg tacctgtgga acttgtcggc
      301 gagcggatgg gcgaacagtt tctactcggc cgccgcgcag gcaggcgcaa gcaattggac
      361 ggcgatgctg ttcggctcga gcgatgccgc caacgcgatc accgtcgaca aaacgccggc
      421 atcgctgtgg atcatggaca tctcggtgcg catcttcggg ctgcacccgt ggagtgtgct
      481 cgcgccacag gcgctcgagg gagtcgccgc ggtcgccgtt ctctacgccg ccgtgcggcg
      541 ggtcagcggg cccggcgccg gtctgctcgc cggtgcgatc atggcgctga ccccggtcgc
      601 cgcattgatg ttccgcttca acaatcccga cgcgcttctg gtcctgatgc tcgtcgtggc
      661 cgcctattgc acgcagcgtg gatgcgaaaa agacagcagc cgctggtggt tggtggcggc
      721 gggggccgcg atcggcttcg gcttccttgc caagatgctt caggcactgc tggtgctgcc
      781 gggattcacc gcggcgtatg cgctcgcggg aagccgcccg ctgggtcgtc ggctcctcga
      841 tgtgctcgca atgctcggcg cgatggccgt ctctgggggc tggtacctgc ttctcgtcga
      901 ggtgtggccg ccggacacca ggccgtacat cggcggatcg cagcacaaca gcatcgtcga
      961 actggcgctc ggctacaacg gcttgggccg gctgacgggt gacgagaccg gcgggctcgg
     1021 caacatgaac ttcgacgtcg gttgggacag gctcctcggg cacagcatgg gcaccgagat
     1081 cgcctggctg ctgcccgcgg cggtcatctg cctgggcgcc atcctggttc tcacccgccg
     1141 cacagcgcgg acggaccaga cccgcgccgc ggcgatcatc tggggcggct ggcttctggt
     1201 gaccgcggtg gtgttcagct tcatgagcgg catcgtgcac ccgtactaca cggtggcact
     1261 cgcgccggcg atcgccgcct gcatcggcgt gggtgcaaca ctgttgtggc agaaccgttt
     1321 cgcgctgccc gccgcgacgg cccttgcggg cgtcgccgtc gtcacctcgg tcctcgcgtg
     1381 cgtactgatg gctcgaaccg acggatggct gccgtggctg cgggccgctg tcgccgtcgg
     1441 cggcttggca tcggcggtgc tgttgctggt gtcgggtcgg cttcgcaccg gcatggcgcg
     1501 atctgtcgcc ggcctggcga tcgtggtggc cttggcggcc ccggcggcct actgcgtcgc
     1561 gacggcggcc acctcgcacg gcggggccat gccctcggca ggcccttcag gcggtggcgg
     1621 aatgccgccg ggagccggtg gcctgctctc cgcaccgacc ccaggccccg ccctctccgg
     1681 cgtattggcc gccaacgccg acgacttcac ctgggcggcc gcggcgatgg gatcgaacaa
     1741 cgcggccggc taccagttgg gcagcggcgc accggtgatg gcgatcggcg
//
LOCUS       DASYLX010001220         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_2631971,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001220 DASYLX010000000
VERSION     DASYLX010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631971"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..524
                     /locus_tag="VGP27_28680"
     CDS             237..524
                     /locus_tag="VGP27_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV7855304.1"
                     /translation="MIGTILSALVVGLIVGALARLIMPGKQSIGLIMTIALGALGAFI
                     GSWLTYQLGYHNANGGWQVIPFLVGIIVAIVLIAIYVGVTGRRGTRTPTVR"
     gene            complement(528..1835)
                     /locus_tag="VGP27_28685"
     CDS             complement(528..1835)
                     /locus_tag="VGP27_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEV7855305.1"
                     /translation="MRIWLVRNGFAAGLVAIMLLAACSSESPPQQSSGSSQTTPAAPA
                     PASAALVSAPVAAAGELAQSPFDEPRQALIPAGWSMSVWARIPKARLEAWTPDGALLV
                     SVPASGEIVKLTPKRGAAPQQSILLDGLDQPHGMAFAGSTLHVAESDQIDAYDYVGGA
                     ATNRRTVAADLPDARSPELQGAYAHALKSVAVGPDGAVYFSIGSTGNISQADRDANPP
                     RATVMRIPPGGDVAQPFATGVRNGTGLAVAPDGSVWTAVNNRDNVPDPEGNVDGQYVN
                     DHPPEALAKLAPGRELGWPYCNPDGGPANLRFIRDMSSNKDGSRMDCASLPPVEQSFG
                     AHAAPLGLAFTTGVLPAPYAAGALAGVHGSWNRQPPRAPEVSFFPWRDGTLADQQTLV
                     GGFQADDGSRWGRPVAAVVGPDGAVYVTDDSAGAVYRLAPPGR"
     gene            complement(1813..2538)
                     /locus_tag="VGP27_28690"
     CDS             complement(1813..2538)
                     /locus_tag="VGP27_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7855306.1"
                     /translation="MTTSVDIRRASDRGTSTTDWLDSKHSFSFAGYYDPSNTHHGLLL
                     VNNDDVVAPGAGFDTHPHRDMEIVTWVLQGSLEHRDSMGNAGVIYPGLAQRMSAGTGV
                     LHSEKNGSADQRVHFVQMWVVPDQSGIDPEYQQTEVDDASLAGGLMTIASGMRDDAAI
                     GLHNKNAALHGARLLPGESVTLPDAPLLHLFVPRGSVTLEGAEQLHEGDAVRFTGAGG
                     QHVTATDSTEILVWEMHADLAGT"
BASE COUNT          452 a          874 c          896 g          431 t
ORIGIN      
        1 cgcaagaagc tttccgctcc tcgcgtgcac ggcggaacgc cgacagactg cggttggtga
       61 cggcacgctt gcccctctag caaaagacca ggaaagaaga caactctaat cacaccaacc
      121 tcacagtcaa cttgcgtaac agcatcgtca cgattgaata tcgaccggat gaggagtgaa
      181 ttcgcgcgcc gattgggtaa tcccgtctgg gtcggaactc accaatagga gcagacatga
      241 tcggaacgat tctcagcgcg ttggtcgtcg gcttgatcgt cggcgcactc gcgcggctca
      301 tcatgcccgg caagcaaagc atcggcctga ttatgacgat cgcgctcggt gcgctcggcg
      361 cgttcattgg gtcgtggctg acctaccagc tcggttacca caacgccaac ggcggctggc
      421 aggtcatccc gttcctcgtc gggatcatcg tcgcgatcgt gctgatcgcg atctacgtgg
      481 gcgtcaccgg gcgccgcggc accaggacgc cgacggtgcg ttaagcgtca acggcccggt
      541 ggggccagtc ggtaaacggc gccggcggaa tcgtcggtga cgtagaccgc gccgtccgga
      601 ccgactaccg ccgcgaccgg acggccccac cgggacccgt cgtcggcctg gaaaccgccg
      661 acgagcgtct gttgatcggc gagagtgccg tcgcgccaag ggaagaacga cacctcgggt
      721 gcacgcggcg gctgccggtt ccacgagccg tgtacgccgg ccaatgctcc ggccgcgtac
      781 ggcgccggta gcaccccggt ggtgaatgcc agccccaacg gcgccgcgtg ggcgccgaaa
      841 ctctgctcca ccggtggcag cgatgcgcaa tccatccgag acccgtcctt gtttgacgac
      901 atgtcgcgaa tgaagcgcag gttcgcggga ccaccgtcgg gattgcagta gggccaaccc
      961 aattcgcgcc ctggcgcgag tttcgccaag gcttccgggg gatggtcgtt gacgtattgg
     1021 ccgtcgacgt tgccctccgg gtcgggtacg ttgtctcggt tgttgaccgc cgtccacacc
     1081 gaaccgtccg gcgccacggc tagcccggtc ccgttgcgga cgcccgtcgc gaacggctgc
     1141 gcgacatcgc cgcccggcgg gatgcgcatg accgtggcgc gcggcgggtt ggcgtcgcgg
     1201 tcggcctgcg agatgttgcc cgtcgagccg atcgagaagt acaccgcgcc gtcgggcccg
     1261 acggcgacgc tcttcagcgc gtgcgcgtac gcaccctgaa gttcggggct gcgcgcgtcg
     1321 ggaagatcgg cggcaacggt gcgacggttg gtggccgccc cgccgacgta gtcgtaggca
     1381 tcgatctggt cgctctcggc gacatggagc gtggaacccg cgaacgccat cccgtggggc
     1441 tggtccagac cgtcgaggag tatcgactgt tgcggcgctg caccgcgttt cggtgtcagc
     1501 ttgacgatct ctccgctggc ggggaccgac acgagcagtg caccatccgg tgtccacgcc
     1561 tccagccgcg ccttggggat ccgcgcccac accgacatcg accagccggc gggtatcagc
     1621 gcctgccgtg gttcatcgaa cggtgactgc gcaagttcac cggccgccgc gacgggtgcg
     1681 gacaccagag ccgcagacgc cggagccggt gcggccggcg ttgtctgcga ggatccggaa
     1741 gactgttgcg gaggcgactc cgacgaacac gcggcaagaa gcatgatggc gacgagtccg
     1801 gcggcgaagc cgttacgtac cagccagatc cgcatgcatc tcccagacga gtatttcggt
     1861 cgaatcggtc gcggtgacat gctgaccgcc cgcgccggtg aatcggacgg cgtctccctc
     1921 gtgcagttgc tcggcgccct cgagcgtcac cgaaccgcga ggcacgaaca ggtgtagcag
     1981 cggtgcatca ggcagcgtga cggattctcc cggcaagagc cgtgcaccgt gcaacgcggc
     2041 gttcttgttg tgaagcccga tggctgcatc gtcacgcatg cccgacgcga tggtcatcag
     2101 gccgccggcg agcgaagcgt cgtcgacctc ggtctgctgg tactcggggt cgatgcccga
     2161 ctggtcgggg accacccaca tctgcacgaa atgcacgcgc tggtcggcag atccgttctt
     2221 ctcggaatgc aggacgcccg tccccgccga catccgctgc gccaggccgg gatagatcac
     2281 gccggcattc cccatcgagt cgcggtgttc cagcgagccc tgcagcaccc aggtgacgat
     2341 ctccatgtcg cggtgcggat gggtgtcgaa gccggcgccc ggcgcgacca cgtcgtcgtt
     2401 gttgacgagc agaagcccgt ggtgggtgtt cgacgggtcg taatagcccg cgaacgagaa
     2461 cgagtgcttg gaatcgagcc agtccgtcgt gctcgtgccg cggtcagatg cgcggcggat
     2521 gtcgacggat gtcgtcatgc acccagcgta cggacggctc agagggaggc cggcaggccc
     2581 aatttcccga acaggccgtc gccggcatgg aaggccacca cgtgcgcgat gccgcccgga
     2641 cgcacctcga gca
//
LOCUS       DASYLX010001221         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U4360 k141_3729722,
            whole genome shotgun sequence.
ACCESSION   DASYLX010001221 DASYLX010000000
VERSION     DASYLX010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958452
            Sequence Read Archive: SRR11678610
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,692
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 51
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 43 of 113
            Pseudo Genes (incomplete)         :: 89 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 29 of 113
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3729722"
                     /isolate="SMAG_U4360"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.783 N 120.2781 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGP27_28695"
     CDS             complement(<1..231)
                     /locus_tag="VGP27_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEV7855307.1"
                     /translation="MTARGTLGGHRGTRIYGRLDCPSALRAIEKGGYVTYRVFFADEA
                     DAVAAGYRPCARCMPEKYRAWKDFRRLRTTPVD"
     gene            complement(228..950)
                     /locus_tag="VGP27_28700"
     CDS             complement(228..950)
                     /locus_tag="VGP27_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV7855308.1"
                     /translation="MAATTTVWQRRVDTADWDAVTTELNDLGGALLPQLVTKAEAAQL
                     RRLYTDDHRFRATIEMSRYRFGEGEYRYFERPYPSPINELKHALYPRLLPIARDWWTK
                     LGRDTPWPDDLDDWLDICHSAGQARSTAILLKYGQGDWNALHRDLYGELVFPLQVVIN
                     LSEPGVDHTGGEFLLLEQRPRAQSRGTATLVPHGHGFVFTTRERPMKSARGWSAAPVR
                     HGVSAVRSGERFTLGLVFHDAA"
     gene            complement(950..1444)
                     /locus_tag="VGP27_28705"
     CDS             complement(950..1444)
                     /locus_tag="VGP27_28705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV7855309.1"
                     /translation="MIDSPLSELTLVADGDALIGLYFRHHWYRPSADTFGPRVAAGSD
                     ALLAESRAQLEDYLTGARTEFDLPTALPGDDRQRRVWNRLATIPYGGTATYGELAAEL
                     ADGTTAQEVGVTVGRNPLSVVVPCHRVVGRNGKLTGYAGGLARKKFLLDLEEPAEVRA
                     ARLF"
     gene            1590..3053
                     /locus_tag="VGP27_28710"
     CDS             1590..3053
                     /locus_tag="VGP27_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEV7855310.1"
                     /translation="MFDSEFAAASDEELVAAIEDGVRLEAVAGARRLSAIAELTRRRT
                     EADDERVLWAFDPWDSTAAEVAAAMAIGARRASGQMRIAEALREHLPQVAALHCKGAL
                     STRLVATITWGTRLVQGDEAWANIDAAIAASARTWERLSEDKLRAAVEMQVARYDPDA
                     KRRTDTNLRGRDFVIGACDDDTETVSVFGRLMASDAAVMKQRVTAMVHGLCDSDPRSA
                     GERRSDAAGAIANHNDTLACRCGSPACPAAGATPKSQVVIRVIAEQAAIDAATTQPAE
                     PAEPAAEQPAAQPARRPAAPALLLGHGVLPNALLAEAIRAGATIKPIRMPSDQPEPRY
                     RPSDELAEFVRMRDLFCRFPGCIVTADRCDLDHARPWPWGPTHPSNLNCKCRKHHLMK
                     TFWTGIGGWADQQLPDATVIWTAPSGKTYTTHPGSRLFYPTWNVTTAELPPTSKPPPN
                     AEYRGLMMPRRKRPRAADIAAAIQAERDLNQRDTAPF"
     gene            complement(3050..3547)
                     /locus_tag="VGP27_28715"
     CDS             complement(3050..3547)
                     /locus_tag="VGP27_28715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV7855311.1"
                     /translation="MRTSTAAVGSAAFFVVAPGTFAGLGPWLITQWEIAGSSPPLRVV
                     LGVVAIVVGLIPPVHAFVQFVGAGGTPMPIAPTECLVVTGFNRFVRNPMYVGLLTVIL
                     GQALLFNSIWLVVYAVIGWIVTASFVRWYEEPTLLRTYGEQYEEYRRNVPAWTPRLTP
                     WKPPL"
BASE COUNT          639 a         1229 c         1186 g          570 t
ORIGIN      
        1 gtcgactggc gtagtcctca gacgtcgaaa gtccttccag gcgcggtact tctcgggcat
       61 gcaacgggcg cagggccggt agccggccgc gacggcgtcg gcctcgtcgg cgaagaacac
      121 gcggtacgtg acatagccgc ccttctcgat tgcacgcaat gccgacggac agtccagtcg
      181 accgtagatc cgtgtcccgc ggtgaccgcc aagagtgcca cgagcagtca cgcggcatcg
      241 tggaagacga gtccgagcgt gaagcgctcg ccggagcgaa ccgccgaaac gccgtgccgg
      301 accggcgccg ccgaccagcc tcgggccgac ttcatcggtc gctcgcgggt ggtgaaaacg
      361 aagccgtgac cgtggggtac aagcgtggca gtgccgcgcg actgtgcgcg tggccgctgc
      421 tcgagcaaca ggaattcacc gcccgtgtgg tcgacgccgg gctcggacag gttgatcacc
      481 acctgcagcg gaaacaccaa ctccccgtac aggtctcggt gcagcgcatt ccaatcgcct
      541 tgcccgtact tgagcaggat tgctgtcgat ctggcctgcc cggcactgtg gcatatgtcg
      601 agccagtcgt cgaggtcgtc cggccacggt gtgtcacggc ccagtttggt ccaccagtct
      661 ctcgcgatcg gcagcagccg cgggtacagc gcgtgcttga gctcgttgat cgggctcggg
      721 tagggccgct cgaagtaacg gtattcgcct tctccgaacc ggtatcgact catctcgatg
      781 gtggcgcgaa accggtggtc gtcggtgtag agacgacgca attgcgccgc ctcggccttg
      841 gtgaccagct gggggagcag tgcaccgccg agatcgttga gctcggtggt gaccgcgtcc
      901 cagtcggcgg tgtcgacccg ccgctgccat accgtcgtcg tcgcagccat cagaacaacc
      961 gcgcggctcg gacctcggcc ggttcctcga ggtcgagtag gaacttcttg cgcgcaagcc
     1021 cgcccgcgta gccggtcagc ttgccgttcc tgccgacgac ccggtgacac ggaacgacga
     1081 ccgagagcgg gttgcggccg acggtcactc cgacttcctg ggccgtcgtc ccgtcggcca
     1141 gctcggcggc gagctcgcca taagttgcgg ttccgccgta ggggatcgtc gccagccggt
     1201 tccagactcg gcgctgacga tcgtcgcctg gaagcgctgt cggtagatcg aattccgtcc
     1261 tggcgccagt cagatagtcc tcgagctgcg cccgcgattc ggccagcagg gcgtctgacc
     1321 ccgcggccac ccgtggtccg aatgtatccg cggatggccg gtaccagtgg tggcggaagt
     1381 acaggccgat cagtgcatcg ccgtcggcga caagcgtgag ttcgctcagt gggctgtcga
     1441 tcaccgcatg tcttgtggtc acatcctgta gacgatcggc gtggcggagg cgtgagatcg
     1501 gagttcaacc gtcagtcatt tccgctttat cctcagccag cgatcctcga cagaaactcg
     1561 tgtcagtgga cccgagtata ttcgaacgta tgttcgattc ggagttcgcg gcggcgtctg
     1621 acgaggagct tgtcgccgcg atcgaggacg gcgtgcgcct agaagcggtc gccggtgcgc
     1681 ggcggttgtc cgcgatcgcc gaactgaccc gccggcgcac cgaggccgat gacgaacggg
     1741 tgttgtgggc gttcgacccg tgggattcga cggccgctga ggtggccgcg gcgatggcga
     1801 ttggggcgcg gcgcgcctcg ggacagatgc gtatcgccga agcactgcgc gaacacctgc
     1861 ctcaggttgc cgccctgcac tgtaagggcg cgttgagcac ccggctggtt gcgacaatca
     1921 cctggggcac ccggctggtg caaggcgacg aggcctgggc caacatcgac gccgcgatcg
     1981 cggcaagcgc ccgcacatgg gagcgcctgt ccgaggacaa gctgcgcgcc gccgtggaga
     2041 tgcaggtggc ccggtatgac cccgacgcca aacgtcgcac cgacaccaat ctgcgcgggc
     2101 gggatttcgt catcggcgcc tgtgatgacg acaccgagac tgtgtcggtg ttcgggcggc
     2161 tgatggcttc cgacgccgcg gtgatgaaac aacgcgtcac cgcgatggtc cacgggctgt
     2221 gcgacagcga tccgcgcagc gccggtgaac gccgctcgga tgccgccgga gcgatcgcca
     2281 accacaacga caccttggcc tgccggtgcg gatcaccggc ctgccccgcc gccggtgcga
     2341 cccccaaatc ccaggtggtg atccgggtga ttgccgaaca agccgccatc gacgccgcca
     2401 ccacccaacc cgccgagccg gccgagcccg ccgccgagca gcccgccgcc cagcccgccc
     2461 ggcgcccggc ggcgccggcg ctgctgttag gtcacggggt gctgcccaat gcgctgctgg
     2521 ccgaggcgat ccgcgccggg gccaccatca aaccgatccg gatgcccagc gatcaacccg
     2581 agccccgcta ccggcccagc gacgagctgg ccgagttcgt gcggatgcgg gatctgttct
     2641 gccgtttccc cggctgcatc gtcaccgccg accgctgcga cctcgaccat gcccgaccgt
     2701 ggccctgggg gccgacacac ccgtcgaacc tgaactgcaa atgccgaaaa caccacctga
     2761 tgaaaacgtt ctggaccggc atcggcggct gggctgatca acagctcccc gacgccaccg
     2821 tgatctggac agcacccagc ggcaagacct acaccaccca ccccggcagc cgactgttct
     2881 accccacctg gaacgtcacc accgcagaac taccccccac ctcaaaacca ccacccaacg
     2941 cggaatatcg cgggctgatg atgccgcgcc gaaaacgccc ccgagccgcc gacatcgccg
     3001 cggccatcca agccgaacgc gacctcaacc aacgcgacac cgcgcccttc tagagaggcg
     3061 gtttccatgg cgtgagccgc ggggtccacg ccggtacgtt gcggcggtat tcctcgtatt
     3121 gctcgccgta ggtgcggagc agtgtgggtt cctcgtacca ccgcacgaat gatgcggtga
     3181 cgatccatcc gatcaccgca tagacgacga gccaaatgct gttgaaaagc agcgcctggc
     3241 cgaggattac ggtgagcaga ccgacgtaca tcgggttgcg gacgaaccgg ttgaagcctg
     3301 tgacgaccag acattcagtc ggagcaatgg gcatcggcgt gccgcctgcc ccgacgaact
     3361 ggacgaacgc gtgcaccggt gggatgagtc cgaccacgat cgccacgacg cccaggacga
     3421 ctcgaagcgg cgggctggac ccggcaatct cccattgggt gatcaaccac ggccccagcc
     3481 cggcgaatgt tccgggtgcg acgacgaaga aggccgctga cccgaccgcg gcggtggacg
     3541 ttcgcaccgc ctaagtatta ggccaacttt gcgcgagttg ggtgagccgg acgctgttac
     3601 ccgacgggtc gcggaaacct gaat
//
